LOCUS       DAOSUT010000001        61769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_326_length_61769_cov_9.532991, whole genome shotgun sequence.
ACCESSION   DAOSUT010000001 DAOSUT010000000
VERSION     DAOSUT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 61769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61769
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_61769_cov_9.532991"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLD05_00005"
     CDS             <1..977
                     /locus_tag="PLD05_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75837.1"
                     /translation="FSSIHLSACGEERSILARIAGTWALCQASSREELLKQQPVLEQA
                     LATPHLRGFCLRVPWKAIDQDFLLLGLGRDLARKHNLAFSVRFMAGRHTPSRVFRDGS
                     PHYTHPGGELVPLPFFSDGRPNMIFERHYEVLIAKLADWCRHNDVPLLHAAWYGCDWA
                     ELDHGQMVRKARGYSFQVWLDAHRRLMDIALKYSGDDLAIELPFSGHGPLTEAALALA
                     DHVISKLGPGNPRFFCQANGWSPRGDWGAPNDDVEAAFDQVWKRPIYRGQQAIQPESF
                     DWSRMYEILRKNRATYCEVYVRSFVLPGKDELARQIEQFAKSPGVKVE"
     gene            1120..2406
                     /locus_tag="PLD05_00010"
     CDS             1120..2406
                     /locus_tag="PLD05_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP75838.1"
                     /translation="MAVTSRERVAMALAHQEPDYVPLDLGGSAVTGMHVSSVYLLRQA
                     LALDPPGTPVKVVEPYQMLGEIDERLQAALGVDVVPLGRRKNMFGFENKDWKPWQLWD
                     GTPVLVPGAFNTTPEPNGDLLMYPEGDKTAPPSGRMPKNGFYFDTIIRQEPIDDEHLN
                     PEDNLEEFTPIDDDELQWLACEADRLYRETDKAILANFGGTAFGDIALVPAPFLKHPK
                     GIRDVAEWYMSTVTRFDYVYRVFERQCEIGIENLKRIYEAVGDRVTAVFITGTDFGQQ
                     HGPFISLKAYRSLYKPFHKRVNDWVHEHTPWKTFIHSCGSVVAFLDDFIEAGFDILNP
                     VQCSAAGMDPATLKQKYGDRLVFWGGGIDTQKTLPFGTPEDVRREVRERMAIFGKGGG
                     FVFNSVHNIQARVPVENLLALFETVNECRGLPFPVR"
     gene            2581..3000
                     /locus_tag="PLD05_00015"
     CDS             2581..3000
                     /locus_tag="PLD05_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOP75839.1"
                     /translation="MTARTRFRLGDLQLKILRILWEKGTASVAEVHASLGKEKFAYTT
                     IATMLRKMENRGLVSHQLENRRFVYFPLVSESQVVRSAASDFVERLFSGSLAAAVCHL
                     LDSCDVTPEELEELEKLIGQRKQTLQEDTKREKRKAN"
     gene            3213..3587
                     /locus_tag="PLD05_00020"
     CDS             3213..3587
                     /locus_tag="PLD05_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75840.1"
                     /translation="MNSEFWYPLLALCLAVGVLGAIAKVIESAFTAARNRRLVWVACL
                     AGMVTATFLLLLGVPFLVRDFLANSSPVISDDVTTGEFWEFTTFNGESYFRSGCYCRS
                     LGLYSVAPATSPRCNGQFRGEK"
     gene            3607..5430
                     /locus_tag="PLD05_00025"
     CDS             3607..5430
                     /locus_tag="PLD05_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOP75841.1"
                     /translation="MSNSSTFARLTPIFLCLWLSGIGLGLGWIGLQRWSIRKLLRTAE
                     PISNTSLQSWLTVQAKRLGFTRRLRVIRCGGLQTPVAFGIVRPTIATPKTFRVEPLDV
                     RSQAILLHELSHLAGSDPFWNLLAALVKALLWWHPMAWIAAKRLKELCEEAADEVTVL
                     LPNGPRELAAGLLECARGRFQTRMPVLAALRTSHARSFIARRIHRLLALEKKDDKAAR
                     VGRIKRIQRFVVITTALAVFLASLGFPVPEVLLTKGEVAMRHRHSLWRHSLMAAAIAA
                     FATPWGMQTLAAEGPGPDQPREVVRQERPDAPRERAAVEGERGPREGDEVTRAEGEGR
                     ERAEGRGPREEGRGPRGPEAIAARLEELRRALREAEEAGQMDRVEQIRREIRSLEAQL
                     ERTREVRRPEGPPPEVRASMMERIERAMNETREKLREAREAGREEAVRDLERRLEQLE
                     RTMRDLREGRRPELPAGLGLETPPAVARERAMERLEQAMNEVRERLKKAREEVREGEV
                     RELEQQLARMEGQMAALREGRPLLPPGAPVPERIERARRAAELLRREGFGDMAELLMR
                     SLEREMRPLPPREPGRVGPPDMPPPPPVRRPDRPEGPPSER"
     gene            complement(5634..6155)
                     /locus_tag="PLD05_00030"
     CDS             complement(5634..6155)
                     /locus_tag="PLD05_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOP75842.1"
                     /translation="MVKSKRNALNRSGVLTLEAVLVLSILILVTLAVFQFAITMLIEQ
                     TITHAVTVAAREAGKGADIDATVESVNAVLQLHGLAIGPGATLILEDPLAIPPVSARG
                     SLDCQPPTQPIVSPGYIRTTLCVSLGQRPFLKLLKTFCLDFSGKIFTVSAVARREFTG
                     PPEVTSRPHCNCQ"
     gene            complement(6251..7507)
                     /locus_tag="PLD05_00035"
     CDS             complement(6251..7507)
                     /locus_tag="PLD05_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOP75843.1"
                     /translation="MNRRGISALWFILTLPLCVILFGFVVELGHLWIARVELENALEA
                     AARAAVDTWAQSGSGTTYPARQVGVAYAAANYVRGIPLQIGLNYEPSPSPDNPNENMT
                     CCVQPGDPLGGILSEGNLVFGTIIRDPNAQCPITFDTRKAPGCSPGTVLVDATAQGPG
                     NLAQDNAWGIVFRYAPNLPSGLTIMRVIIDLQASGGSGRFDFTSSGPVLSDSSPAPII
                     PCHYDVEGFTNPTTQISFTPTSGYSRTLTIDFFPDPDTGDLGFEPGDRIRFGARTTGV
                     SKGSGQDDGDGIGRDGVTVTVVFAINGVPLPAVTRTFIDNEETSNDCQPTCPVHPSGI
                     DDLPCPPASSPENNGQSYVILGGGSGGDYAVRAQAVVAVPSPICKLFGLRMPRYRVSA
                     KTIAIHSCEEQRTRLQRVDQYICVAP"
     gene            complement(7653..8192)
                     /locus_tag="PLD05_00040"
     CDS             complement(7653..8192)
                     /locus_tag="PLD05_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOP75844.1"
                     /translation="MIDTTHRRNSPGIRPIRNGPARREGALTLELVLVLPMLLVMILA
                     VVQFGQYLANVQELALAARVGGEEAAISANLPTTEGAPVPSAVVTAIDQQLLAAGIVH
                     RMIVLEHNVGRHQVSLVTPPGADPPPKLASVPPGQYVRVLIIVPKSEVMPNLLKTLGL
                     NLVMPDSTTLVSYVMKHEG"
     gene            complement(8447..9982)
                     /locus_tag="PLD05_00045"
     CDS             complement(8447..9982)
                     /locus_tag="PLD05_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP75845.1"
                     /translation="MVRLKWLRFVLAVVGLASINMGDAAAQEDSPPNIVFIYADDLGY
                     GDIGCYGATRIPTPNLDRLAKEGLLFRDAHSPAATCTPSRFALMTGQYAFRKPGTGVL
                     PGNADLIIEPGQLTLPLMLKRAGYITAVVGKWHLGLGRNGCDWNGTIAPGPLEIGFDY
                     AFIIPATGDRVPCVYVENHHVVGLYPNDPIQVSYGEKIGDEPTGREHPELLKYRFSHG
                     HDGTIINGISRIGFMKGGKSARWVDEDMADVITQKAVSFIESHKDQRFFLYFATHDIH
                     VPRLPHPRFVGKSQCGLRGDAVVELDWCVGQILDTLDRLSLTNNTLVIFSSDNGPVVD
                     DGYHDGAVEHLGDHRPSGPFRGGKYSAFEGGTRVPMIVRWPNKIAAGTKSDALVCHID
                     FLASFAALVGQSLPEDVAIDSVNVLPALLGKTQVGREELVEQAGGLALRQGNWKYIPP
                     RKGPAINKNTNIELGVDPNGFLFNLENDPGEQNNLISSLPDKAKAMAERLQAIRDAKQ
                     GGP"
     gene            10361..11194
                     /locus_tag="PLD05_00050"
     CDS             10361..11194
                     /locus_tag="PLD05_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOP75846.1"
                     /translation="MSLDTTAMNHPKEVHKRDPLFRFQMFTSLSLLVLFLIVTLSGAI
                     LYSSPRGRVANWGQWTVLGWDKESWAAFHINATICFVVFSILHLLFNWKMLLGYLKRG
                     TLGLVRGRLELLAALVFAGLLYAGTRYHVPPFSQFVAWREAFKDYWERRSPPAPIPHL
                     EEFTLERISQLAGASEEEILQFVRSLGVEDVKSSSTLAQIAAQIGQSPRQVFDAMAQR
                     FPSLQAIQPPSPEQRRGLRRPDGSALEGPGAFGPGSGRGGGRGMGQGLRRGRMSDPNF
                     E"
     gene            complement(11260..11883)
                     /locus_tag="PLD05_00055"
     CDS             complement(11260..11883)
                     /locus_tag="PLD05_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOP75847.1"
                     /translation="MSGKSSEIRNYFSRLIDELNRLNLRELEDFSQVLFDAWLHRRLV
                     FVCGNGGSAATASHLAQDLAKGTIRDADLLSYAVPRFKAISLVANISWLTALGNDLGY
                     EQVFVQQLANYAQPGDVLIAISGSGNSPNILAAVEWANDHELMTLGMTGFDGGRLRAM
                     QKKGIHVPLNDMEMVESIHSCICHWLVNDLRQRIYEAIVSSATRESK"
     gene            complement(11998..12384)
                     /locus_tag="PLD05_00060"
     CDS             complement(11998..12384)
                     /locus_tag="PLD05_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOP75848.1"
                     /translation="MKIVIAIVRPFVAEKILEALKFAPVEACTVREVKGFGRQKNYLE
                     EYRGGDYALAFIPKVQITIWVDDLRTEEIIRLIVSNARTGRMGDGKVFVLSADFTGTE
                     SFDLARAGEHEENEELPEDGFTGFFG"
     gene            12742..13038
                     /locus_tag="PLD05_00065"
     CDS             12742..13038
                     /locus_tag="PLD05_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75849.1"
                     /translation="MKDNKPSRSAVNRTPYQEGIIRRYYENRDNLMIQKLGDLVGELY
                     LSEGKARARIWKRITAALRNLSVPEARIEHLVKSDNPEFVAKILEELQTRGQVD"
     gene            13113..14006
                     /gene="thyX"
                     /locus_tag="PLD05_00070"
     CDS             13113..14006
                     /gene="thyX"
                     /locus_tag="PLD05_00070"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOP75850.1"
                     /translation="MSTNISVVEQLRWRKLPVLDDGFVCLVDVMGDDSAIVQAARVSY
                     GAGTKKVSDDRTLIRYLMRHRHTTPFEMAEVKLLVRVPMDIWRQWIRHRTASVNEYST
                     RYSIAIDTAHRTPPDKWRKQSTTNRQGSEGFLDLQTGEELSREERELQEKARQVYQHR
                     LDLGVAREQARKDLPLSTYTEAYWKIDLHNLLHFLELRMDRHAQWEIAQYARVIGYEI
                     VAPLFPMTWEAFVDYRLQGLVLSRLDCEVITRLVARGSLPATEKDFLEAQDPAWRGLT
                     RCRERDECWEKLKRLGIVKDP"
     gene            14482..>16931
                     /locus_tag="PLD05_00075"
     CDS             14482..>16931
                     /locus_tag="PLD05_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOP75851.1"
                     /translation="MSLGSMGVAVARNVEEDSSGEGLPTIVKRLEFPDAVSLVELSNG
                     LTIIVQENHSVPVATVRCYVKYTGSIYEAEYLGAGLSHVLEHVVSGGTTTKRTEREIA
                     AMIDRFGGKSNAYTSTNFTCYYIDCPSKNVNEAIELIADAMQRVAFEPSEFERELKVV
                     KQELADGEVNRSRVLWKLLNQTLYLQHPARHPVIGYLDVLNRTSNEAIINFYKSRYVP
                     NNQIFVVVGDVTVDEIVKSIAKQYQGSKRAPETYLPLQEEPPQLTPRRVVREMEGDVV
                     DFVIAWPTVKLSDPDLYPLDVAADLLSRGDSSRLVRRLQREQPLALQVDAVSATPEYV
                     RGWFGVMARCLPDRFEEVRNIIFQEITRLRDEPVSEAELERAKKKKISDWVFERESVQ
                     ASAESLARGFISTGDPLFDKQYTERIQAVTAEQIREVARKYFRPEWLNEVIIAPLGRS
                     PATVKADQTSDETAARLVRLNNGLRVVVKRQPRLPIVTMQIAILGGNLVDTPETAGRS
                     SLLAMMLDKGTERLTAEQVAEMFDDLDARVSFTPGRNTLLVGLSILKDSFTPAAELLA
                     ECLLKPRFSEDEFAKVKVLALDAIAQRTANPQAEAQELFADLLPEESPYHVVMGGKKE
                     TVERITINDLKNLHAQILNPEQMVVAVYGDCDPERAVELVDRLFGGLRPPANAPRISF
                     DRSNKIARIISGHKQIQKPTSVIVMGYPCPGFHDEKDYLAMMVLDTIMSGYDYPGGWL
                     HEELRGEGLVYGVHALQLTGPVPGYFVIIAQTRPEAQNEVITRILNNVDKAKRGQISE
                     EEFLRAKELLVAYHAQKNT"
     assembly_gap    16932..17031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17032..17297
                     /locus_tag="PLD05_00080"
     CDS             <17032..17297
                     /locus_tag="PLD05_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75852.1"
                     /translation="EFLRAKELLVAYHAQKNTTIEEQAQQAILDELYGLGFDYDRGFE
                     TRIQAITLDDVKRVAREYFGNYVLATVSPLKSLPEEASTGGDR"
     gene            complement(17376..18737)
                     /locus_tag="PLD05_00085"
     CDS             complement(17376..18737)
                     /locus_tag="PLD05_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75853.1"
                     /translation="MKTSVWIMLVFAFGLLAPPCFSQGLTEERLRQLELETQRLRAEL
                     ESLQESRPVAFPPIQVDQTGFVGAPQDAAQTPSMDEIRAEMKKIAWTKGDIQIIPYGY
                     LWATTAYESSRSQVGDYVLYVLSNTDQGESAWHVDAKSTRLGIDFLGPVLPNFGEAQT
                     GGKVEIDFQGQYQTENKGTVLLRHAYLEVKNEYFRLLAGQTWDVISPLLPGTIMYSVG
                     WGAGNLGYRRAQFRAERYLHFSDTLLVTTQASINSDVVSEFAGNVIGDHADWPVVEGR
                     LAFTLGPRGKGCRPVEIGISSHIGEQVYDFLAAPVQDDVEFRTWSFNVDAKWPITSRF
                     GVQGEFFTGENLGAYLGGIVQGINLTQRDTIRSTGGWFDIWYDWTSRLHTHAGYSIDD
                     PLNGDLSPGQRSYNQFYYVNLTYDVTKKFVLGIEVQQWKTNWVNKEPGDSYRLELMAK
                     YGF"
     gene            complement(19257..20096)
                     /locus_tag="PLD05_00090"
     CDS             complement(19257..20096)
                     /locus_tag="PLD05_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP75854.1"
                     /translation="MPRPELSVVIPCKNEDHHLEDCVLSVVDWADEVVIADSGSTDRT
                     LEIAARLCERFPTRCRLVQREFINFGSFKSWASQQCRHDWVFVLDADERMSKSLSHEI
                     DTVLSSSSAADAYLVRFRHYFLGHRIRFGKWNGRGVIRLFRQSRCHYDSREVHEKVIV
                     PSGRVRKLHSRLDHFTCTSLDRWFQKKYAYARYGANELAKRGRRARAYDLFLRPLIQF
                     IAGYIFRLGFLDGIPGLIVAFDGAFSTYLKYVKLWEIQQNQKSSPIQQNPQILPDSKK
                     QAA"
     gene            complement(20122..21177)
                     /gene="waaF"
                     /locus_tag="PLD05_00095"
     CDS             complement(20122..21177)
                     /gene="waaF"
                     /locus_tag="PLD05_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOP75855.1"
                     /translation="MNICIFLPNWLGDLVMAVPALRAIREATGSSAYVVGITRPNLAG
                     LLAGSQWLDEEWYFDPRGDDPRFETATLIEKLRSVHWDVGILFTNSFRSALLAVAGGI
                     PKRVGYSRHGRRLLLTHPVSVHEVHGTGRYIPMVDYYLALARVAGFPSQDRRLELPLS
                     PWGTVCADRIWRDLSLDSSDQVVAMHVAGAYGAAKSWPFSHFVELARMIATRTSASVL
                     FVCGQRERATVQRIAREVNHRRVVSMADHPLDLQTLKACLARCHVMVSTDSGPRHVAA
                     AFGKPVITLFGPTSPIVTANPTVQSIDLQIPIDCAPCSCRTCPKGHHRCMKGLSPELV
                     FEHLQPFLIQQNMRGAA"
     gene            21794..22312
                     /locus_tag="PLD05_00100"
     CDS             21794..22312
                     /locus_tag="PLD05_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOP75856.1"
                     /translation="MRAEFINPFIHSLYKTFSTMLNCSVQRGQLSLKTSSKTSYEISG
                     VIGLTGRAVGAVVLTLSKPVALKAASTMLLCDFSEINEDVIDAVGELANMVAGAAKAE
                     LEEYALVVSLPNVVTGKDHEIHFPSNVTPICIHFSSDWGPLALEVGLATIQNPSPEAA
                     TLTHHSEAVAAS"
     gene            complement(22338..23729)
                     /locus_tag="PLD05_00105"
     CDS             complement(22338..23729)
                     /locus_tag="PLD05_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOP75857.1"
                     /translation="MGRASDIRIVGVNSWTEEYSYRTPMKFGGRVVRDVVLRNVVVEV
                     ETRTGRRGRGFGSMPVGNVWAWPSQVLSAEMTLGAMLLFGEKMVKVVGDYQGRGHPLE
                     ITEDWATEYGKLAASVEAEQSLSEPMPKLAQLVAASPFEAALHDAYGRALNLNAYNTL
                     SSEFVNADLAHFLSPEFKGEYLDTYTYRQPKAKMPLYHLVGALDPLTDQDLSERLNDG
                     LPETLLEWIYANGLTHLKIKLNGDDLRWDVDRVLAIHRVTSEAQRARGCDTWHYSADF
                     NERCESVDYVLEFMTRIRENSPEAFDRLQYLEQPTHRDLKRFPENRMHKAAALKPVVI
                     DESLVDFESLLLSRELGYSGVALKTCKGHSEALLMAAAAQKFGLFLCVQDLTCPGYSF
                     LHSASLAARIPGVAAIEGNARQYCPSANRGWDEKYPTMFRITDGTVGTHVLDGIGLGF
                     EPLFWSKEQIGSR"
     gene            24075..24623
                     /gene="nuoE"
                     /locus_tag="PLD05_00110"
     CDS             24075..24623
                     /gene="nuoE"
                     /locus_tag="PLD05_00110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOP75858.1"
                     /translation="MPDDAKPAGVFSAPCACQEVSEEDRYAQLDDILRTYRNIPGALI
                     PVLQITQTIFGYLPEKALRKIAAALNKPLSEVTGVVTFYSFFNTIPRGRHMIRVCLGT
                     ACYVRGGKQVLAALKEELGIDVGETTADRNFSLDVGRCFGACGLAPVIMIDDDVHQRV
                     KVSQLPRLLEQYRDKQDTTSKT"
     gene            24719..27886
                     /locus_tag="PLD05_00115"
     CDS             24719..27886
                     /locus_tag="PLD05_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP75859.1"
                     /translation="MAEESPKRNRVSSVNDLNAWRNQLLAEQKRQSDLGIKELRICTG
                     AGCIASGSLHTREEIVRQLQDRDLANRWKVISTGCMGPCFGGPVVMIGDVVYERVRAE
                     DAATIVVEHLGQGKIVDRLTHRRADGRHVAHLKDIDFFKRQVKIALRNCGVIDPGCIE
                     DYIARDGYQALAEVLEKRDPDAVIETLRESGLRGRGGAGFPTWRKWAFARQQEAPTKF
                     VVCNADEGDPGAFMDRSILEGDPHSVIEGMAIAAATIGAQKGFIYVRAEYPLAVQRVQ
                     VALSQAREVGLLGENILGLGFDFDIEIRMGSGAFVCGEETALMTSIEGNRGEPRPRPP
                     YPVEKGLWGHPTLINNVETLANIPIIILKGGAWYAQWGTPKSRGTKVFALAGGVKHAG
                     LVEIPIGMPLGDLIYDIGGGTASGKPFKAAQIGGPSGGCIPRQHLNVPLDYESLAELG
                     AIMGSGGLIVMDDDSCMVDVARFFLEFVQEESCGKCVPCRVGTKRMLEIMERICAGQG
                     EEDDVERLIELGEMIKETSLCGLGQTASNPVLSTIRHFGQEYVEHIRDRHCRAGVCSA
                     MVAAPCESACPAKVDIPGFVALVAEKRYAEAIRLHRERNPFAAVCSRVCFHTCEDKCR
                     RATIDAPVAIRAIKRFMVDQEITLQVPEIREDPKNAKRKIAIIGAGPAGLTCAYFLAR
                     LGYRPEVFEAEQRPGGMLVQAIPAYRLPREVVAREVRMIEQMGVEIITGIALGRDFTL
                     TELRAQGYEAVFLGVGAPEGTRLGIPGEDVQGVEDALTFLRTYNIRGSVRVGKRVVVV
                     GGGNSAIDAARTAIRLGAEMVTVVYRRNREAMPAYEEEITEAEAEGVRLQLLTAPVEV
                     LSEGGQVVGLKCVPVQLGEFDRSGRRRPKEVGEAFCIPADQILVAVGQTLDLERLVPD
                     VELRRNSRGFIEVDPVTGQTSHRWIFAGGDAVNGPSSVVEAVAAGERAAVGIDMLLTG
                     QSHAFWRETKPVETSFDPDAEPEATPREPLRLLPVERRRHNFDEVELPWPEPVALRQA
                     RRCLRCDYGKRNGVQGPVLHQVGEL"
     gene            27966..29726
                     /locus_tag="PLD05_00120"
     CDS             27966..29726
                     /locus_tag="PLD05_00120"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOP75860.1"
                     /translation="MVRIYINDQEYDVPAGTTILCAAKKAGIRIPTLCFVEGLRPIGA
                     CRVCVVEVEGVRNLVASCSTPVAEDMRIHTHTARVRRARRAVVELLLSEHNGECQTCE
                     RNGQCELQSLAAELGIREVSYRGEKARKRIDISTPALQRDSGKCIKCRRCVSVCNEMQ
                     HVGALFPQFRGYQTIIGPALGRDLASVPCVQCGQCAAICPVGAISERDHVEAVWAALD
                     TPENHVVVQTAPAIRAALGECFGYPPGTLVTGKMVAALRRLGFDAVFDTNFAADLTIM
                     EEGTEFLMRLRKAYVEGQQDVLPMFTSCSPGWVQYLEFFHHNLLPNLSTCRSPQQMFG
                     AIAKTYYAKKLDKRPEDIFVVSVMPCTAKKFEASRAEMNASGVRDVDAVLTTRELARM
                     IRIAGIDFQSLPDEEMDAPLGLCTGAGVIFGHTGGVMEAALRTVYEVLTGEDLPTPTL
                     LATLDGIKEAEITLKNVRPEWSFLEGVTLRVAVAHGLGNAEKLIRAIEAGERQYHFVE
                     VMACPGGCIGGGGQPRFTDNRVRLARIAAIRKEDAGKPLRKSHQNPQVKQLYEEFLGE
                     PLSERSHKLLHTHYIAREPV"
     gene            complement(29813..30187)
                     /locus_tag="PLD05_00125"
     CDS             complement(29813..30187)
                     /locus_tag="PLD05_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75861.1"
                     /translation="MRQRFLISSVCVVILTVSVPLVASAGENTFFGRRAAQRARSYSW
                     SGEYYGATWGAPIAVVVPPTAEFQTHWGWGVGNFRVTPIDHQFSRNWPGPAQGGYLPL
                     KPTPQWPTDTDQFGMYYIRGPW"
     gene            complement(30244..30594)
                     /locus_tag="PLD05_00130"
     CDS             complement(30244..30594)
                     /locus_tag="PLD05_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75862.1"
                     /translation="MQIRKLLGWKIGLFFFAAVVSWASTSPSMAGDGSFVMGRYQGSH
                     AGDLFPSYWAPAAPGGISAPLYVSPIPTPPHVGHVWVTYQPLYPHEFLYKHSKVYWRK
                     NPQGGWTRVVVGWY"
     gene            30866..32980
                     /locus_tag="PLD05_00135"
     CDS             30866..32980
                     /locus_tag="PLD05_00135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOP75863.1"
                     /translation="MVKSTEEMSLETSPEKRSGPQNGNGQTRSPRIRVTAESMAAKQR
                     EISVSEFFAKNRHLLGFDNPRKALLTMVKEAVDNALDACEEAGVLPEIWVRIDPTGEN
                     RYRVIVQDNGPGIVKKQIPLIFGKLLYGSKFHRLRQSRGQQGIGISAAGMYGLLTTGK
                     PVSIVSKTGPKKPAHFYQIQIDTKNNRPEIVNGRGEGIDIPPQEDIGQFLVPYGLPWS
                     DVPHGTLVAIEIEAKFQRGRGSVDEYLYQTAIANPHVTIHYVDPEGKSRDFSRVADVL
                     PPEPKEIKPHPYGVELGWLLTMLKDTKASTLSGFLVDSFSRISPAVAQRIAETAQLST
                     RANPRKISRHDAEALFRALQETKIQSPATDCLSPIGEELILKGLRELVPGEFYTAITR
                     PPAVYRGNPFQVEVGLAYGGAPATYRVSLEVLEELLAETDARTLRQFLTTTFDGIGPE
                     GADRIIHQARLKSRMSPAKLKAREREQLCQALQTVNLSEGQTMTILRYANRVPLLFQA
                     GACAITQTVMSTNWRPYGLTQSRNALPSGPVTLMVHFASVWVPFTSESKEAIAAYPEI
                     QRELRLAYQAVGRRLGLYLRRRLRVKQQGERRNIFLRYLKEVANAVAVIRNVDRESVY
                     RDLMEVAKRVTAEADLKLDEHGNPIEETPSSEEFDGAVIIVEQQQGNPPLRSGNSKPI
                     QLQLFEDNGENGEPSPQDEEAT"
     gene            complement(33151..33678)
                     /locus_tag="PLD05_00140"
     CDS             complement(33151..33678)
                     /locus_tag="PLD05_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOP75864.1"
                     /translation="MPQLQEMYVVLKYGFIMHVTKILDCLYVGSCPTSQTDVQELKEL
                     GITAVLNLQTDEDIAWRNIDRDGVSAAYRAYRLREIRYPIRDFDYEDLRRRLPEAVRL
                     LHNLVKEGHRVYVHCTAGMNRSPTVIIAYLHWAMNWDFPEAVQHVTRNHPCDPFLEAI
                     SEQSPPEWDEDLTGS"
     gene            complement(33772..35160)
                     /locus_tag="PLD05_00145"
     CDS             complement(33772..35160)
                     /locus_tag="PLD05_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP75865.1"
                     /translation="MFQSESRNHGNGRATDRRSFLKSAGTLGVGAATVLTGVQVFGES
                     SPSQQIVLGIVGCGGRGTSLATGFLERGDCRIAWVCDPFLPRAEALAKTVEQLQGARP
                     QVSDDMRRALDDKAVDAIISATPDHWHALSTVWACQAGKDVYVEKPTSHNAWEGRKMV
                     EAARKYQRVVQIGTQNRSAPYNWQAKEYISSGKLGRIHLCRIFNQKLWENFPVRPDSD
                     PPAGLNWDIWNGPAPEAKYNPNYQYHWHHFWRYSGGDIANDASHQIDLARWLLGVDYP
                     KTVYSVGGRFDSEGAAETPDTQIAVYEFDGLVVTFELTLYTPYMLKIDPEVRNSDMFP
                     LWQHCATRIEIYGSEGVMYVGRHGGGWQVFVRPKSREPVVAAQCYGRFPDPEHKANFV
                     DCIRTRKLPNADVEEGHRSALWIHFANISYRLGGVKLVIDPKAEQITNVPEAAALYRR
                     EYRPPYVIPDNV"
     gene            complement(35242..35715)
                     /gene="tadA"
                     /locus_tag="PLD05_00150"
     CDS             complement(35242..35715)
                     /gene="tadA"
                     /locus_tag="PLD05_00150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOP75866.1"
                     /translation="MGDEDIHVHFMRQALREAEQAKHENEVPIGAVIVKNGRIIAAAH
                     NQREQLKDPTAHAEMIAITQAAEAIQSWRLEDCTLYVTLEPCPMCAGAIIMARIPVVV
                     YGADDPKGGAVRSLYRLLEDPRLNHRCVVISGVLAMECGTILTQFFQSQRAMGKK"
     assembly_gap    35721..35820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36016..36843
                     /locus_tag="PLD05_00155"
     CDS             36016..36843
                     /locus_tag="PLD05_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP75867.1"
                     /translation="MRFAICNETFNDRSVEAGFELAAECGYEGIEIAPFTLAAYVTEL
                     TPQRRTEIRRSIEKCGLTTVGLHWLLAKTEGFHLTSPDPAVRARTLEYLKALAECCAD
                     LGGRILVFGSPQQRNVLPETNRLQAEAFAIEVLEGLLPTLERTGTIVALEPLGPQETN
                     FVNTAEEAVAIIKKLSSPHIQLHLDCKAMASEAIPIPDLIRTNKQYLVHFHANDPNRL
                     GPGFGELDFVPIFKALLDAGYQGWVSVEVFDYQPGCERIARESIAYMQRCLTEAQKR"
     gene            complement(36948..37511)
                     /locus_tag="PLD05_00160"
     CDS             complement(36948..37511)
                     /locus_tag="PLD05_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOP75868.1"
                     /translation="MDRSGLATGYVLFATLVGFLVALWVVFAITAPRSQFVWAGLSVA
                     EEKGTVQEKAPPDNSPCFVCHANFEDEPLSTRHFKAKIGCVDCHGESLAHRSDENNTT
                     PPDKMYAPDEIDPACKSCHKNHNASAREVVEVYLERCREITDPKKLTCTNCHGRHRMY
                     HRTIVWDKRTGKLITPTESTEDPHQSP"
     gene            complement(37570..39168)
                     /locus_tag="PLD05_00165"
     CDS             complement(37570..39168)
                     /locus_tag="PLD05_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP75869.1"
                     /translation="MFGKRVPGYPHLVLLSVFFGSITASFVTTLAEERPNILFCLADD
                     WSYPHASAYGCPWIQTPGFDRVAKEGILFTRAYTPNAKCAPSRSIILTGRNSWQLKEA
                     CNHNCEFPLEFKVVTEVLEEHGYFVGKTGKGWGPGRARDPNGQPRPLLGKSFDKHTTT
                     PPTPTISRNDYAANFAEFLKEKPQDRPWFFWYGSTEPHRAYQFQSGLNLTGKKLSDVN
                     WIPPYWPDNDIVRTDLLDYAFEVEHFDRHLQKMLELLEQAGELENTVVIVTSDNGMPF
                     PRVKGQCYELSNHMPLAIMWKKGISHPGRVVDDFISFADFATTFLELAGINAAQSGMA
                     AFAGRSFLDILISDRVGQVDPSRSFVLVGKERHDVGRPHDWGYPIRGIREGQWLYLRN
                     YEPSRWPAGNPETGYLNCDGSPTKTEVLHARRENRNTAYWKACFGKRPPEELYNVVED
                     PECSRNLIKEYPEVAQRLSQKMTEILRQEGDPRMFGQGDIFEKYPYIDPRTQNFYERF
                     MSGEEVRAGWVNPDDFEPVPISDE"
     gene            complement(39627..40052)
                     /locus_tag="PLD05_00170"
     CDS             complement(39627..40052)
                     /locus_tag="PLD05_00170"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOP75870.1"
                     /translation="MADVELLMESDVTKENLAALFRRAFFKTTFDSDGDLVVETDGPR
                     VLVTIDEERRLLKYMAIYGVLEWSPLEEKLELSNALNDKFIFARFSIPEKRDDVLIAD
                     YFLPYEGGIPAFQIIAAVRLFSRVVPAAIRVCDENNIVE"
     gene            40341..41681
                     /locus_tag="PLD05_00175"
     CDS             40341..41681
                     /locus_tag="PLD05_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOP75871.1"
                     /translation="MMSQPLEKPVSSSPSGKRRWYQAIGPALITACVVFGPGSLIISS
                     NVGAVYRFQFLWLLILTGLLMGTYVAMAARIGVCGGASPCTLLARRLGRPVAFFVGMV
                     LCLVCATFQFSNNLAVALAAGAFLPERINLTIGGTVQQLPVLPTLLIILNGMIILFLF
                     VGRQIYKTLERGMKVMVAIILLCFVFNLVVARPDVIGVLRGFIPSWPEGLQLGIPKKI
                     GQTIDDPMILLASLLGTTFSVGGAFFQGNLVREKNWSIHDYQHGIIDSIAGVAVLTGV
                     SMIIMITAATVIPGKPAHDVGELARSLEPLLGPTSYTLFCLGLFAVAMNPIVINAVIG
                     GTIFADGLGLPARLNDRAPRILTVLVMLIGMGVALWALTSGQRPVPLIIFGQALTVIG
                     NPLMAAALLWLANQPDIMGEKRNRLVSNLLGGLGFLVVLLVAARVLWRVILHFA"
     gene            complement(41790..43334)
                     /locus_tag="PLD05_00180"
     CDS             complement(41790..43334)
                     /locus_tag="PLD05_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75872.1"
                     /translation="MNWLRTAYRLFSFFDALYLAGNFLYRRSLRYTFTTAFVSFVGYL
                     GNLVPGVKTYDARVAILMPLLVGSFMLTTGLLLKLIPLAFRSRLLNVAQAADLDLMEN
                     YRKWNQEMHLESLWERVYQYEWKLGTHLTGVRPHEAECPSELCSDEGLPSDVVERGRI
                     KFLRRARFGLARPQPESRQRHHLGIDLRFMEDWYNGGYFDPNDVKLDEQQAAAVPIMI
                     VRSLVHYGFWDSVRDLPLRLFGRIWFRLITRSIALRVGEAVIRLNRQFNTDYFNAQAL
                     LWPEESEEPWVAALGEDARQALLRERARILRCVFNNPTEGVLMLDRFLVPLFLFATDL
                     RARFDPEYLDGSLGYDLWSDLKWVGFPAFKRMHFARLIQRAARESRLIKEVLASQEMG
                     DLTPHPLGEGNQEILRAIRIATFINWEQLGTLMRRFGKKRKYTPELRRVIREKLQKVI
                     ENRHRFSAYLVSIRVHHELTRLHRATYHRLLLDLFRTCEEIPRDLNAQCSRLITQGQS
                     LLDLDS"
     gene            complement(43470..44501)
                     /locus_tag="PLD05_00185"
     CDS             complement(43470..44501)
                     /locus_tag="PLD05_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP75873.1"
                     /translation="MKLRLGVIGLNDQWEKRYLPALRVLEDRFVLTAIYEPVAQRAAL
                     AARDVGITTHDSFRTLCRRPDIDAVLVLNTHWLGPLPILAACEYGKAVYLGSRVVLSL
                     EETTQIRDAVRQSGVAFMVEFARRYSPATVRLKELMATVLGSPRLLFCHRRLSHYLKC
                     NGLSHITPSQALHLDLIELVDWCRFVAGRSPIKVMGVVHRSSHPDDVDYMMMSLNFGS
                     ASEGREPNVLAQISCGKYLPPHWREAVNYRPLAELQVVCERGVAFVDLPTTLVWFDEA
                     GRHQESFDNERPLGERLLFCFHRMVTSLVQRMSDIEDLHQTQRIIESLEQSARTGQCV
                     FLSSERHKG"
     gene            complement(44562..45686)
                     /locus_tag="PLD05_00190"
     CDS             complement(44562..45686)
                     /locus_tag="PLD05_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOP75874.1"
                     /translation="MSRQSIFEASTRYFLSPILPYLEDDSVSEIMINGPFEVYIERRG
                     KLEKTDARFPSEDALISAINNVAQWVGREIGPEHPVLDARLPDGSRVHAILPPTSRIG
                     TCVTIRKFTKEAMTLEDLVRLNSLSEVVKEFLEICVRIRKNILISGGTGTGKTVLLGA
                     LSRAIPFEERIVVIEDTSELRFVQPHTIYLEARHGGREGQGMTIRQLFSNSLRMRPDR
                     IIVGEVRGGEALDMIQSMLSGHSGSLSTIHASSPRDALIRLETLSLMSDVDIPVYVAR
                     AQVASAIHLVVQIARFTEDGSRKITHVTEVAGLDAENRYHLRDLFVSQLVGRNPDGSL
                     VADLKWTGIQPMFANEPAQQGLLGHVRLSKSLWAPEATDR"
     gene            46105..46177
                     /locus_tag="PLD05_00195"
     tRNA            46105..46177
                     /locus_tag="PLD05_00195"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46138..46140,aa:Thr,seq:cgt)
     gene            46374..47702
                     /locus_tag="PLD05_00200"
     CDS             46374..47702
                     /locus_tag="PLD05_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOP75875.1"
                     /translation="MAEATLGADVRQIVLSNAAFGPRDVKALADAMAEDSSQYRALRD
                     AVAELELREETPASNVRLGCCLYLLGHFERAIDVLKRGDNGALAHYYLGRAYIATQKF
                     DLALESFRAARIAGYNSDECALGEVEALRLKGNAAEALQILDRLSGPVVESAEYLFQR
                     GATLACLGASRDEVIELYERAVQSDPYHVGALFGLGLENDRQGNDEVALELYQRCVSR
                     FPPHLGALINLGILYEDAEQYEKAVRCYQRVLDAFPDHPRARLFLKDAQAAGRIVLDE
                     DVARRKDRLAQILATPVTDFELSVRSRNCLQKMGIMTLGDLCRCTEQDLLASKNFGET
                     SLAEIKEMLASKGLRLGQLLPERAAVEPLGEPEEPLAPEEEALLAKPVSELNLSVRAR
                     KCMARLGINTVGELIRKSGDELLECKNFGVTSLNEVREKLRALGLKLRGD"
     gene            47815..48951
                     /gene="tgt"
                     /locus_tag="PLD05_00205"
     CDS             47815..48951
                     /gene="tgt"
                     /locus_tag="PLD05_00205"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOP75876.1"
                     /translation="MHSAQESFQVICEDPHCAARCGILRTAHGEVQTPAFMPVGTRGS
                     IKGVLPELVCRTGTQIILSNTYHLLLRPGPEIVAELGGLHRFMGWDGPILTDSGGFQV
                     FSLAQLAKIDETGATFRSHIDGQRVVLTPEGSISVQEMLGSDVAMVFDHVVGLPNERT
                     VIKEAMERSLRWAERCKVAHSRKEQLLFAIVQGGLEPDLRIRCAEALINLGFDGYAVG
                     GLSVGEPPQEMYRILDVTVPVLPRTRPRYLMGVGRPVDLIEAIYRGIDMFDCVIPTRN
                     GRNAMAFTDDGPMRMRNARYERDPRPIDPTCPCPACSFSRGYIRHLFMVKEMLGPILL
                     TLHNLTYYQRLIQRARSAICEGRFCEFRQEMLSKLAAGNDANDT"
     gene            complement(49007..50095)
                     /locus_tag="PLD05_00210"
     CDS             complement(49007..50095)
                     /locus_tag="PLD05_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOP75877.1"
                     /translation="MSENSPLVRQWILLRMLASRRFGVTVREMAQTLEVSEKTIRRDL
                     DMFIQAGFPLDETVGEHGKKRWRLTGDLIEKGLPITYDEAIALHLARCLMEPLAGTMF
                     WTAAQSAFNKIRSVFNETAWAYVEKMAHSFYRTRTGMCDYAKKAELIDQIMLAIEDRK
                     AIELRYHSLKAPEATTYTVHPYGLTYHRGALYLVGLLVECNEIRHWKVNRVEAVKITD
                     QPFDFPEGFSLEKHLAGSFGIFQGDGDYHVRVLFSPVVARYVSEATWHASQRLYPQRD
                     GSVIAEFDLDSLEEIQRWILSFGKYAKVLEPEELQQRMQEEIREMLATYEDLRERPSR
                     KRQLSPHAKLAFAAPDFGPAFAPIERTP"
     gene            complement(50460..52427)
                     /gene="dnaX"
                     /locus_tag="PLD05_00215"
     CDS             complement(50460..52427)
                     /gene="dnaX"
                     /locus_tag="PLD05_00215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOP75878.1"
                     /translation="MAARSKKVKPNEETVTSQAKSGSTSTGYFVVARRYRPQTFEELV
                     GQDHVATALRNAIVSGRVGHAYLFTGARGVGKTSTARILAKALNCVKGPTPEPCNMCD
                     ICRSIGSGDDIDVIEIDGASNRGIEEIRQLRQNAVIRPSRGRYKIYIIDEVHMLTKEA
                     FNALLKTLEEPPEHVKFIFCTTEPNKVPVTILSRCQRFDFAGIETQTIVARLQQIVEA
                     EGATVEPGVLELLARRAAGSMRDAQSLLEQLLSFSGGKNIAVSDVLELLGMARAEVFK
                     AILEAATKEDASQALAVFHQALKDGADAGLLIEQLLTYLRDCMVATLGCSGDMLLSVG
                     PGELSVASELGQRLGLPRLLASMQLLDHTLARMRFATHDRILAELAIVRLSRLGELEY
                     VGKLIEAIRSGKSLPTVATPDQARSVEGIGISQPLHQAPKTLASELAGVLGRPSPGSP
                     QPPAGDQQGAAQARQAVEQAKAELERQKEAVKASVPREGTLPAYPSGEALTSENVLNV
                     WVKAAESVGGLAADQAKQFSQVAVAGGNRVVVTFRKDKAFCRDYCSRPEIKTRFERAL
                     QTITGNKIELELVLANEGAPEKSSPGTGERLLFDILQEPFVRKTAELFGARPIAVLPP
                     EERKTTVTQAPIEEDRSLDEEHHVEEDEDDV"
     gene            complement(52567..52665)
                     /gene="ffs"
                     /locus_tag="PLD05_00220"
     ncRNA           complement(52567..52665)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD05_00220"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            52877..53698
                     /locus_tag="PLD05_00225"
     CDS             52877..53698
                     /locus_tag="PLD05_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOP75879.1"
                     /translation="MPVEKAVVLARGLGTRMRKEDASVILSEEQEKIARKGIKALIPV
                     AAGRPFLDYVLSALADAGITRVCLVVAPDHDELVARYGGEVPLERLSVSFAIQEKPLG
                     TANAVAAAEEFVNGDPFLMVNSDNYYPHDALAALRELSGPAVALFEQEAMLTQSNIAA
                     ERILSFAVGLVDANGYLVRILEKPSRETLAHLPRPIWLSMNCWRFGPSIFEACRRIGP
                     SPRGEYEITDAVQYAIDHLQERFQTVCVKKAVYDMTSRADIPAIVARLEGIKVWL"
     gene            complement(53731..54747)
                     /locus_tag="PLD05_00230"
     CDS             complement(53731..54747)
                     /locus_tag="PLD05_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOP75880.1"
                     /translation="MISFRVGSDRYEGQLLYWTSDEIFVLGRDGALVRAERRLARDFH
                     VVNDDFVALDPGAIRAQLLREFGRSYTVDTTSHYIVVRPASMTEDCREVFEATYRNVQ
                     RFAAQRKLPLQESSFPLIAIVLASRAEYLDYLARLKKDSDALSNTLGCYDPVSNRVVT
                     FVLDAPQQRSGNSPTSGSKLPNVTRHEAFHQVATNMGLVNRFADVPLWFHEGMALMFE
                     GDFVNPISKVSGRAISQPQDLKLQARHVLAKQSPGWLRRLVLSDSLFSSSPETAYGLS
                     WILVTYLHKKHHEGFVRYLTTINRLAPFETLSPQRRVADFVEAFGDRWEALESAVRKF
                     AGEM"
     gene            55122..56291
                     /locus_tag="PLD05_00235"
     CDS             55122..56291
                     /locus_tag="PLD05_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOP75881.1"
                     /translation="MAIYLDHNATSPIRSEVAEAVREAWFTYHGNPASQHASGRAARA
                     RLASAREKILALLDADTSHNGDRIIFTSGGTEANNLAILGMAMARTGGAPGRIIVSAI
                     EHPSVLRAAEHLLDLGWRLDTLDVSRDGLINLDSLRAWIGPDVALVSVTLANHETGVI
                     QPVAEVAAICHFHGVPVHTDAVQAIGKIPVSFRLLGVDAMTVTAHKCGGPLGIGALIV
                     RRDQPLLPILFGGEQQDGLRPGTESVALAVGMEVTFELALHELDDTMPRMRSLQAQFE
                     DYLKREIADIVVHGQGAPRLPQTTSLAVRGIDNQLLLAALDAEGIECSIGSACSSGSA
                     EPSATLLAMGVPRDLVRSSLRFSFGPRTTQEELITAASTLADLVRRLRGKRQTPR"
     gene            complement(56329..57783)
                     /locus_tag="PLD05_00240"
     CDS             complement(56329..57783)
                     /locus_tag="PLD05_00240"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOP75882.1"
                     /translation="MRLHVTIVFGVCLALAFHGVRTVSAEEWTTVKPKEIDAILPNPH
                     MGWETFHRTADRDQNLPEWIPSTVQYARWGWGRLEPRPGEIDYEFLDQQLAESRRAGQ
                     RLAFRVMCCSTTPKRPYHPDWLKAIGGRVITTRYGDTEGLEVPDLDDPVVLRAHLDFI
                     RRLGARYDGHPDLDHVDLGSVGWWGEWHMSRSDPAKMPTPENQKAIIDAYLASFKKTP
                     LLMLIDGPEMLRYATERGTGWRADCLGDMGGFSANWCHMRNAYPQTLTKANALDVWKR
                     APVAWETCWDIRKWVDEGWSLRYIFNYALALHASYINNKSAPLPPGDEVKAEVERFLR
                     RLGYRLVLRSLSHPREIGRGQQLPITMEWQNVGSAPCYRAYPVAYRLRHKTGKSFVIV
                     SDNVTVNQFLPGSVDVFTPAFLAQPPELPPGEVMNVKDTLTVPATVPLGEYELAIGVV
                     ESASRPVPVLRLAIEGTTADGWYPLSRISIRAAN"
     gene            complement(57841..59253)
                     /locus_tag="PLD05_00245"
     CDS             complement(57841..59253)
                     /locus_tag="PLD05_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75883.1"
                     /translation="MKQLTRRRFVQLAGASLAAGILNSNRALPASLQVPGERPVRDSA
                     IRVLNPRNRVPVSLIIDDSTCLVNLAHFAMPQFAEVWPGRAEYQKPWKEFPREIPDSF
                     VRRFMDWCEEHGVKGKYSVVPYPACVGWLDRDLPGWSHQELVESLRLVRERIMPRWDI
                     HPEMVTHTWVINTKTGRPYEERSARFMENWGWSEGRSVDELTDYLAYALRILKNVDLH
                     CEGITTPGGFGSRARPQLAQATLEACRDVFRTEIPHYFRDLFTDERSVAPRVEYASGL
                     DGDDPKCVVSIIGCTGDWFGGWDGTTEGSADLFITPDLAKGRMVDVIGREEPAIMVCH
                     WPGIYFNGEERGFEILKTVVQRLHARYENLIWMNLAEIARYWAARELTRIEKNDTKVV
                     FSAPFSAPLFTIQLTPLAEGVPAVGRAGEEQQRLRRVGRLLDLTSGTYCEGSGTNGTE
                     RLMTLCFDLPKGTSELVWLG"
     gene            59529..59888
                     /locus_tag="PLD05_00250"
     CDS             59529..59888
                     /locus_tag="PLD05_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75884.1"
                     /translation="MNYEESPPEFDDEPVDDLDSDQEDGELPPPPPVSPEDDFVEFDE
                     DDFDDDFDDDFEFEPEYEAEIEREFGDIDQGEEIPEETEAEEEPEEPEKVLQEPGEFP
                     EGDLTDDEFTESDEEEF"
     gene            59973..60824
                     /locus_tag="PLD05_00255"
     CDS             59973..60824
                     /locus_tag="PLD05_00255"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOP75885.1"
                     /translation="MSETLVEGIQAAVQFIRQKWDKEAYAGIILGTGLGTLAQEIKAD
                     AVIRYEDIPHFPRSTTISHTGQFVCGTLEGLPIVAMEGRFHVYEGYTFQQVTFPVRVM
                     KALGANLLIVSNACGGMNPHYRLGDIVVIDDHINLMNGNPLIGPNDDSLGPRFPDMSQ
                     PYDQTLIDVALAIARREDFVAHRGVYVAVTGPNLETRAEYRFLRGIGADVVGMSTVPE
                     VIVAVHAGMRVLGLSVVTDMCLPDALKPAVVEEILKVAAEAEPKLRKIVLGVLRFEAE
                     KQQRRPG"
     gene            60971..>61769
                     /locus_tag="PLD05_00260"
     CDS             60971..>61769
                     /locus_tag="PLD05_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOP75886.1"
                     /translation="MFRPVDRNPSFPKLEEGIIRFWKENRIYERSLDWRRGAPSFVFY
                     EGPPTANGLPHPGHCLTRAIKDLFPRYKTMRGYYCERRAGWDTHGLPVEVEVCKELGI
                     HSKKDIEEFGVEPFIRRCIDSVFRYTREWEQLTERLGFWIQLDNAYVTYHQSYVESVW
                     WALKQLFEKGLLYQGYKIVWWWAQGGTALSAAEVGQGYRSVADPSVYVRFPLRDEPDA
                     DLLVWTTTPWTLPSNQFAAVRPDLDYCHVLFEGEKRAVVIAQALVETV"
BASE COUNT        14089 a        16591 c        16996 g        13893 t
ORIGIN      
        1 gcttttcgtc aatccatcta tcggcctgcg gggaggaaag gtcgatcctt gctcgaattg
       61 ccgggacctg ggcactttgt caggcctcca gccgcgaaga actcctgaag caacaaccag
      121 tgcttgagca ggcgttggca acgccacatc ttcgaggctt ctgtttgcga gttccctgga
      181 aggccatcga tcaagatttt ctactcctgg gtttagggcg cgatctcgct cgaaaacata
      241 acctcgcgtt ttccgtgcgg tttatggcgg gacgtcatac gccaagccgc gtgttccgag
      301 atggaagtcc tcactatacg caccccggtg gtgaattagt ccccttgccg tttttttcag
      361 acggtcgtcc caacatgatt tttgaacgtc attatgaggt cctcatcgca aagctcgcgg
      421 actggtgtcg tcacaatgat gtgccactcc ttcatgctgc gtggtatggt tgtgactggg
      481 cggagttgga tcacggccag atggttcgca aggcccgggg gtattccttt caggtgtggc
      541 tcgatgccca tcggcgactg atggatattg ccttgaaata ctccggcgac gacctcgcta
      601 tcgagctacc gttctcaggg catggtccgt tgacagaggc ggcgctcgcc ttggccgacc
      661 acgttatcag taaacttggt cccggcaatc ccaggttttt ctgtcaggct aacggatgga
      721 gccctcgcgg cgattggggg gctccgaatg atgacgtgga agctgctttc gaccaggttt
      781 ggaaaaggcc gatttatcgc ggccagcagg ctattcaacc agaaagtttt gactggtccc
      841 gtatgtacga gatcttgcga aagaaccggg ccacttactg cgaggtctat gtgagaagct
      901 tcgttcttcc gggtaaggat gagctggcac ggcaaattga acaatttgcc aagtcacccg
      961 gcgtgaaggt tgagtgacca ttattactcg gcggctttcc gttctggttt gtggcgaaat
     1021 cacgcgaggc cgtgtggagt cgcaacgtgg gccattcgcg ttataatgct ccttgttgga
     1081 aaatgagata ccgttgggtc ctcatcaagg aggcattgca tggctgtgac ctctcgtgaa
     1141 cgtgtggcga tggccctagc gcatcaagaa ccggactatg ttccgctgga tctaggcgga
     1201 agtgccgtga caggcatgca cgtcagcagt gtctatctcc tccgtcaggc cttggcactt
     1261 gatccgcccg gcacgccggt caaggtcgtg gaaccatatc aaatgctcgg ggaaatcgat
     1321 gagcggttac aagcagcctt aggggtcgat gtggttcctt tgggacgccg aaagaacatg
     1381 ttcggcttcg aaaataagga ctggaagcct tggcagcttt gggacggcac cccggttctt
     1441 gtaccgggcg ctttcaacac gacgccggag cccaatggtg acctgcttat gtatcccgag
     1501 ggcgacaaga cggcaccgcc cagtggacga atgcctaaga acgggtttta cttcgatacg
     1561 atcattcgcc aggaaccgat cgacgacgaa catttgaacc cggaagacaa ccttgaggag
     1621 tttaccccga ttgacgatga cgagctccaa tggttggcgt gtgaggcgga ccgcctttat
     1681 cgtgagacgg acaaggctat tttggcgaac tttggcggta cggcctttgg cgatattgct
     1741 cttgtgccag caccattcct gaaacatccc aagggaatcc gtgatgtggc ggaatggtac
     1801 atgagtacgg ttacgcgatt tgattatgtc tatcgcgttt ttgaaaggca gtgtgaaatc
     1861 gggatcgaga acctcaaacg catttacgaa gcggtggggg atcgagtaac tgccgtgttc
     1921 attacgggca cagactttgg ccagcaacat gggccgttta tcagcctcaa agcctaccga
     1981 tccctttata aaccgttcca taagcgggtt aacgactggg tacacgaaca tacgccatgg
     2041 aaaaccttca tccactcatg tgggtcggtg gtggcatttt tggacgattt tatcgaagct
     2101 gggttcgata tcttgaatcc cgtccagtgc tccgcagccg gaatggaccc agcgaccttg
     2161 aagcaaaaat atggtgatcg tttggttttc tggggcggtg gcatcgatac gcagaagacc
     2221 ctcccgtttg ggacccccga agacgtccgg cgagaagtgc gggaacggat ggccatcttt
     2281 ggtaaaggcg gcggtttcgt attcaatagc gttcacaata ttcaagcccg cgtccctgtt
     2341 gagaatctcc ttgccctttt tgagactgtc aacgagtgtc gcgggctgcc ttttcccgtg
     2401 cgttagtgga cttgttggtg aatatcggag agtgtccaat cgctgcctgc acatggtcgt
     2461 ttggacaggg tcctggtcgt gcaaacgcag attcgcgacc ttcgaggggc ttgacaagtt
     2521 ttcgcgaccg tggtagtatt accatggtag ttttcaacgc cttcaaccga ggaatggagt
     2581 atgacagcac gaaccagatt ccggctgggt gaccttcagc ttaagattct gcgaatcctt
     2641 tgggaaaagg ggaccgcttc ggtggcggaa gtgcacgcca gcttggggaa ggaaaagttt
     2701 gcctacacca ccatcgccac gatgctgcga aagatggaga atcgggggct ggtgtctcac
     2761 caactggaga atcggagatt cgtttatttc cccttggtca gtgaatccca ggtcgtccgc
     2821 tcggcggcat ccgactttgt tgagcggctg ttttctggca gtttggccgc ggctgtttgc
     2881 catctcctcg atagctgtga tgttacaccg gaggagttag aggagctgga aaaactgatt
     2941 ggacagcgca aacagacgct ccaagaagac acaaagcgag aaaaacgcaa agcgaattag
     3001 attctatccc aaaagttctc cctctccctt tgggagaggg ttggggtgag ggaaaaggca
     3061 cctcggaatc aattgagaaa accagcagcg atccgaggcc ccccatcccc cacctctctc
     3121 cggccgagca ggtgagggga gattatgaga tagaccctta atgtggtgcc attggttatg
     3181 cgtcccagga gccatcaacg agattatcag ccatgaacag cgagttttgg tatccattgt
     3241 tggcgctgtg tctcgcagtg ggggtactgg gggccattgc caaagtcata gaatccgcct
     3301 ttacggcggc gagaaacaga cgacttgttt gggtcgcctg tcttgccggc atggtcacgg
     3361 caactttcct gttgctattg ggcgtgccgt ttcttgtgcg cgattttttg gcaaacagta
     3421 gcccggtgat ttccgacgat gtcaccaccg gcgaattctg ggagttcaca acttttaacg
     3481 gagaaagtta ctttcgaagc gggtgttact gtcgatcatt gggcctctac tcggtggcgc
     3541 cagcaacttc accaaggtgt aacggtcaat tccggggaga aaagtgaaaa agacatcact
     3601 agtcctgtgt cgaattcttc taccttcgcc cggctcacgc ctatattctt gtgcctttgg
     3661 ctatctggga ttgggctggg gcttggctgg attggcctcc aaagatggtc gatccggaag
     3721 ctgctgcgaa ctgccgaacc gatctcgaat acctcgctcc agagttggtt gacggtgcaa
     3781 gcaaagcgat taggttttac ccggcgtctg cgggtgattc gttgcggcgg tttgcagact
     3841 ccggtggcgt ttggcatcgt ccgtccgacc attgctaccc ctaagacttt tcgtgtcgaa
     3901 ccattggacg tgcgcagcca ggcgattttg ctgcatgagt taagtcatct tgcgggtagt
     3961 gatccctttt ggaacttgtt ggcggctttg gtcaaagctc tcctctggtg gcacccgatg
     4021 gcctggatcg cggcgaaacg tttgaaggaa ctgtgcgaag aagcggcgga cgaagtgaca
     4081 gtccttttgc ccaatggccc acgggagttg gccgccgggc tgcttgaatg tgctcgtggt
     4141 cgatttcaaa cgcgtatgcc ggtacttgca gcacttcgaa cgtcacatgc gcgttcattc
     4201 attgcccgac gtattcaccg gcttcttgcc ctggagaaaa aagacgataa ggcggcccgg
     4261 gtagggagga tcaagcgaat tcagcgattt gtcgtgataa caacagcctt agctgtgttc
     4321 ttggcgtcgt taggatttcc tgttccagag gttttgttga cgaaaggaga ggtagctatg
     4381 cgacatcgac actctttatg gcggcactca ctgatggccg cggcaattgc ggcgttcgcc
     4441 acgccgtggg gcatgcaaac cctggccgcg gaaggcccag ggcccgatca gccgcgggaa
     4501 gttgtccgcc aagagcggcc ggatgccccg cgcgaaagag cggctgtgga gggagaaagg
     4561 ggacctcgcg aaggtgacga ggtgacacgt gccgagggtg aagggcgaga aagggccgag
     4621 gggcggggtc cacgtgaaga aggccgagga ccacgaggac cagaggcaat cgcggcaagg
     4681 cttgaagaac tacgacgagc attgcgcgag gcggaagaag ccggtcaaat ggatcgcgta
     4741 gaacagattc gccgcgagat tcggtcgctt gaggctcagc ttgagcgaac gagagaggtg
     4801 cgtcgtccgg aaggcccacc gcctgaagtc cgagcatcga tgatggaacg aatcgaaagg
     4861 gccatgaacg aaaccaggga aaaattgcga gaggccagag aagcgggacg cgaagaagct
     4921 gttcgcgatc tggagcgtcg tctcgaacag ttggagcgca ccatgcgcga tctgcgtgaa
     4981 ggacgccgcc ccgagttgcc agcgggtctg gggctcgaaa ctccgcctgc ggttgcgcgg
     5041 gaacgggcga tggagcgact ggagcaggcc atgaacgaag tccgcgagcg actcaagaag
     5101 gctcgggaag aagtccgcga aggtgaagtt cgtgaactcg agcaacagtt ggctagaatg
     5161 gaagggcaaa tggccgcact gcgagagggt cggccactcc tgcctcctgg agcacctgtt
     5221 cccgaacgta tcgagcgagc acgccgcgca gcagaattgt tacgacggga aggttttggg
     5281 gatatggctg aattgttgat gcgctcctta gaacgcgaaa tgcggccttt gcctcctcgt
     5341 gaacccgggc gggtaggccc tcccgatatg ccaccgcctc cgcccgttcg gcgccctgat
     5401 cgaccagaag gcccgccatc agagcggtga ttgacaaccg ctgaaccctt agcagagtga
     5461 ggctacgcgt ctcgcactcg gtgcgagaaa acgcgaaagt gggacctggg gtgcagtttg
     5521 agtactgcac cccatttgtt ttcgctaggc ttgtctggaa aaggttggga acatgtatct
     5581 cttgcggaac tcgcactgtg ttccgttgct gccgggccgc aacctttcgc ggcttactgg
     5641 caattgcagt ggggacgaga ggtgacttct ggtggaccgg tgaactcccg gcgtgcaacc
     5701 gccgacaccg tgaatatctt accggaaaag tccaagcaaa atgtcttcag caacttcaag
     5761 aaagggcgct gtccgagact tacacagagc gtagtgcgaa tataaccggg agagactatc
     5821 ggttgtgtcg gcggctggca gtccaagcta ccccgagcac tgacgggagg tatcgcaaga
     5881 ggatcttcaa ggatcagagt agcccctgga ccaatagcaa gcccatgaag ttgcagcacc
     5941 gcgttgaccg attccacggt agcgtcaatg tcggcccctt ttcctgcttc gcgagctgcc
     6001 accgttacgg cgtgcgtgat agtctgctca ataagcatgg tgatcgcgaa ctgaaaaacg
     6061 gcgagcgtca ccaggatgag gatagaaagc accagaacgg cttctagtgt cagaacgccg
     6121 ctacggttta acgcatttcg ttttgactta accatggttt ggctctctcg ggcactattt
     6181 caaagctttg cgaagcctct cgaccgcact cccacagaga actgggtgag ctatccttgg
     6241 tgagctcgct ttatggcgca acgcagatat actgatcgac cctctgcaaa cgtgtccgct
     6301 gctcctcgca ggagtgaatg gcaatggtct tagcggatac ccggtatcgt ggcatccgca
     6361 aaccaaaaag tttgcaaatg gggctgggaa ctgccaccac ggcttgagcc cgcacagcgt
     6421 agtccccccc tgatccacca ccaagaataa cgtacgattg accgttattt tcgggagaac
     6481 tagcgggtgg acaaggtaag tcgtcaatac ccgatggatg gacaggacat gtgggctgac
     6541 aatcgttcga tgtctcttcg ttgtcgataa atgtccgcgt tacggctggc aaaggaacgc
     6601 cgttaatcgc aaagacgaca gtgaccgtta caccatcgcg gccaatgccg tctccatcgt
     6661 cctgcccact tcctttagac acaccggtgg ttcgtgcacc aaaacgtatg cgatcgcctg
     6721 gttcaaatcc taggtcacct gtgtcaggat cggggaaaaa atcaattgtc agagtacgtg
     6781 agtagccgga cgtcggtgtg aaactaattt gggttgtcgg attggtaaaa ccttccacgt
     6841 catagtggca ggggatgatc ggagcagggc tactgtcaga aaggacgggg ccactcgatg
     6901 taaaatcaaa ccgtcccgat ccaccactgg cttggagatc aatgataacc cgcatgatgg
     6961 tcaaaccact tggcaggttt ggagcgtaac gaaagacgat tccccaagcg ttatcctgag
     7021 ccaagttccc aggcccctgg gccgtggcat caacgagcac tgtacccggg ctacatcctg
     7081 gggcctttct cgtatcgaat gtgatggggc attgtgcgtt gggatctcgg ataatggttc
     7141 caaacacaag attcccttca gaaagaatcc caccgagggg atcgcccggc tgaacacaac
     7201 acgtcatatt ctcgttgggg ttatccggac ttggtgaggg ttcgtagttc aagccaatct
     7261 ggagaggaat accacgaaca taatttgccg cagcgtaagc aacacccact tgccgagccg
     7321 ggtaggtcgt tccactgccg gattgtgccc acgtatcaac ggcagccctg gcagcggcct
     7381 cgagcgcgtt ttccaattca acgcgtgcga tccataggtg tcccaactct acaacaaatc
     7441 caaacaagat cacgcaaaga ggaagcgtca ggatgaacca taacgctgag atacctcggc
     7501 ggttcatagg agtgtgatgt ttgctcattg tgaacctatt gaacagtaaa taaccacagt
     7561 agtatgcgtc ctctgggagg aaccagacga cgcgactatg gttcctttga gtactcggca
     7621 gacaaggaca aggactggac ctggcgttcg tgttacccct cgtgcttcat aacgtacgag
     7681 acaagtgttg tactatccgg cataaccaga ttaagcccga gagtcttaag gagatttggc
     7741 atgacttcac tcttgggcac aataatgagc acacggacat attggcccgg cggaaccgag
     7801 gccaatttcg ggggaggatc agccccggga ggcgtgacca aactgacctg gtggcgtccc
     7861 acgttgtgtt cgaggacaat catccggtgc acaatcccgg cagctaggag ctgttgatcg
     7921 atcgcagtga ccacggcaga tgggactggt gcaccctcgg ttgtgggtag attcgcggaa
     7981 atagctgcct cttctccgcc aacgcgggca gcaagggcca actcctgaac gttcgccagg
     8041 tactggccaa actgcacaac cgcaaggatc atgacgagca gcatcggcag caccagcaca
     8101 agctcgagcg taagagctcc ctcacgacgg gcagggccgt ttcgaatggg cctaatacct
     8161 gggctgttcc ggcgatgcgt cgtgtcaatc atgggggtta ggtctcaccg cttcggaggt
     8221 acgcttgcgt atgtgtagcg gctgcacgtg taccgaaagc tgtttatcga tactgctggc
     8281 gagcaaaatt gattccaata ccaaaggaat cgtggacgta ccgacaggtt aagaaaagca
     8341 aacgtcaaac gttccaaatg ggcaacgtta tccttacaac acgttgccct ttggcaacat
     8401 atcatggacc tggttgagta cccgccgcaa tggtgctcgc tgattgttac ggtccgcctt
     8461 gcttggcgtc acgaatcgct tggagacgct ctgccatggc cttggcctta tccgggagtg
     8521 acgagatcag attgttttgc tctcccgggt cgttttcgag attgaacaaa aagccgttcg
     8581 gatcgacacc taactcgata ttggtgttct tattaattgc aggtcccttt cgcggtggga
     8641 tgtacttcca gtttccctgt cgcagcgcca atccgccggc ttgttcgaca agttcttccc
     8701 tacccacttg ggttttaccc aaaagggctg gaagcacatt cacgctgtcg atggcaacat
     8761 cctctggtaa ggattggccg acaagggccg caaacgatgc gagaaaatcg atgtggcata
     8821 ccaaggcatc acttttcgtg ccggcagcaa ttttgttggg ccaacggaca atcatgggaa
     8881 cccgcgttcc accctcaaac gcgctgtatt ttcccccgcg aaagggacca ctaggtcgat
     8941 gatctcccag atgttccacg gcaccatcat gatacccgtc atccactact ggcccattat
     9001 cactggaaaa aatgaccaag gtgttgttag tcaaagagag acgatcaagc gtgtcgagaa
     9061 tctggccaac acaccaatct aactccacga cggcgtcccc ccgcagacca cattggctct
     9121 ttcccacaaa cctgggatga ggtaaacggg ggacatgaat atcgtgcgtg gcgaaataca
     9181 agaagaatcg ctgatctttg tgcgattcaa taaatgacac ggccttttgt gtgataacgt
     9241 cggccatgtc ttcatcaacc caacgagcac tcttgccacc tttcataaaa ccgattcgac
     9301 tgatgccatt gataatggta ccatcgtgtc cgtggctgaa acgatacttg agaagctccg
     9361 gatgttcgcg ccctgtgggt tcgtcaccga tcttttcacc gtaactgacc tgaatcggat
     9421 cgttgggata gaggccaact acgtgatggt tttcaacata cacgcaggga acacgatcac
     9481 cagtagccgg aataatgaat gcataatcga agccaatctc caatggcccg ggtgcgatcg
     9541 tcccattcca atcacaacca ttccgaccga gtcccaggtg ccatttgccc acgacagcag
     9601 ttatgtaacc tgctcgcttg agcatcagag gaagagtcaa ctggccaggt tcgatgatca
     9661 ggtcggcgtt ccccggaaga acaccggtgc ccggtttgcg aaaggcatat tgtccggtca
     9721 tcagggcaaa acgagatggc gtgcaggttg cggcaggcga atgggcatcc cgaaagagaa
     9781 gcccctcttt ggcaagacga tccaaattgg gtgtaggaat ccgcgtggcc ccataacagc
     9841 cgatgtcgcc gtatcccaaa tcatcggcat agatgaagac gatgtttggg ggactatctt
     9901 cctgtgccgc tgcgtctccc atgttgatag atgccaatcc aaccaccgcc aggacaaatc
     9961 gaagccattt caatcgaacc atagcgcctt cctttcaaga agtcgtgaat tcgaatcgat
    10021 tgcaggaatc cacatgtgat agctaacagt acaccgaatg taatgagaaa gagacgactg
    10081 ttcaacctgc ctcgtacttg ggtcgtctgt cgtgcggagc cccgcgagca agatgtggct
    10141 gtctattttc acccgatgaa tctatgacaa gagtagccac tggtaggttg agcggtgcgc
    10201 aattgttgca taggcagaag accgtactta atcgggtact cataacataa tattaaatca
    10261 taacttacga ttcgacttga tgtggtatct cgtttgctac aatgatgcgg ggaaggggct
    10321 ctgttgatca gaaaacacca aactggaaaa ggactctgct atgtctttgg acacgacggc
    10381 catgaatcac cctaaagaag ttcacaagcg cgacccgctc tttcgattcc aaatgtttac
    10441 ctctctgtcg cttcttgtat tgttcctaat cgttaccctg tccggtgcca ttctttatag
    10501 ttccccccgt ggacgggttg ccaattgggg acagtggacg gtattgggtt gggacaagga
    10561 gtcttgggcg gccttccata tcaatgcaac catctgcttc gtggtgttca gtattcttca
    10621 cctccttttc aattggaaga tgttactcgg ctatctcaag cgtggcactc tcgggctcgt
    10681 tcgtggtcgt cttgaactgt tggccgctct cgttttcgcc ggactccttt atgcgggtac
    10741 gcgttaccat gtgccgcctt tttctcagtt tgtggcctgg cgagaggcgt tcaaggacta
    10801 ctgggaaagg cggtcaccgc ccgcaccaat cccacatttg gaagagttca cacttgagcg
    10861 aatctcccaa ttagccgggg cgtctgagga ggaaatcctc cagttcgtac gatcgcttgg
    10921 tgtcgaggat gtgaaaagtt caagcacgct tgcccagatc gctgcacaaa tcggacagtc
    10981 tcctcgtcag gtattcgatg ctatggcgca gcgatttccc tcactccagg ccattcaacc
    11041 tccctcgccg gaacagagac gaggactccg taggcctgat ggcagtgctc tggaggggcc
    11101 cggagccttt ggcccagggt ctggccgtgg gggtggacgc ggaatggggc agggcctccg
    11161 ccgtggtcga atgagtgatc ccaactttga gtgacttccg ggaacagccg tccttcttgt
    11221 gaccattgcc cggcgcagtg gtaccacttt gtgaaaggat tacttggatt cccgcgtggc
    11281 tgaagagact atggcctcgt aaatgcgctg acgcaggtcg ttgaccaacc aatggcaaat
    11341 gcacgagtga atgctctcga ccatttccat atcgtttagc gggacgtgaa tccccttttt
    11401 ctgcatggca cgcaatcgtc ctccatcgaa ccccgtcatg ccaagcgtca taagctcatg
    11461 gtcgtttgcc cactcgactg ccgccaagat gttggggcta ttgcctgagc cactgatcgc
    11521 aataagaaca tcaccgggct gagcgtagtt cgccagttgc tgaacaaaaa cttgctcata
    11581 gcctagatcg ttgcccaacg cagtgagcca actgatgttc gcaacgagac ttatggcctt
    11641 aaaccgtggc acagcatacg agagtagatc ggcatcgcgg atcgtgcctt tggcaaggtc
    11701 ttgtgccagg tgacttgccg tggccgcgga acctccattc ccgcagacga acaccaaccg
    11761 ccggtgcagc caggcatcga acagcacctg agaaaagtct tccagttctc gcagattgag
    11821 gcggttcagt tcgtcaataa gtcgcgaaaa atagttgcga atttctgagc ttttgccgga
    11881 cacgtatcgc actccttcta agcatcatct accggccctt tcgcggaatc tccggtctca
    11941 ttgatggtat atacccgccg gcgagatgcg tattctacaa atgatcgttg aaaaatctta
    12001 cccgaaaaag ccagtaaacc catcttcggg cagttcttcg ttttcttcat gttccccagc
    12061 acgcgctaag tcaaacgatt cggtacccgt aaaatctgcc gaaaggacaa agacctttcc
    12121 atcacccatt cgtcctgttc gggcattgct gacaatgagg cgaatgatct cttcggtccg
    12181 caggtcgtcg acccaaattg tgatttgcac cttgggaatg aaggccaggg cataatcgcc
    12241 gccgcgatat tcctccaaat agtttttctg acgaccaaaa cccttgactt cccggaccgt
    12301 gcacgcctcg accggcgcga acttcagggc ttcgagaatc ttctcagcaa cgaacggtcg
    12361 aacaattgcg atgacgattt tcatgcgctt ctttcgctat ggttcctaac cagagcgacg
    12421 cggtcctacc cggccagacg actcacctca tgtatgtcct tgacggacca aaagccgctt
    12481 ggccgcggcc aaagcattaa gtttatcatt tcatcgccag acacaatagc gatcctaggg
    12541 ggcctactct aagagttgac gaccaaaaga cataatgaac acagaggaga atcggcaggt
    12601 ggttggggtg acggtgactt ccccaagaat tgctcacaaa ggatgccgcg gcggtttgac
    12661 gataatggct agtatggatc gtggcagtaa cgaactgcag gtttgagacc agcattgttt
    12721 aagatgggaa gcaatatgag gatgaaggac aacaagccgt cgcgctctgc tgtaaatcgt
    12781 acgccttacc aagaagggat catccggcgg tattacgaga accgggacaa tctgatgatc
    12841 cagaagctag gagatctggt tggcgaactc tatctgagtg agggaaaggc aagagcgcgt
    12901 atctggaagc ggattaccgc ggcacttcga aatctaagcg tcccagaggc gagaatcgag
    12961 cacctggtga agtccgataa cccggagttt gtcgccaaga tcctggagga acttcaaact
    13021 cgtggccaag tggattaaaa ggttatatcc ccacttgtgt cgcggttgcc cattcaggac
    13081 tgctaggtcc agaaaagtga agatttggga gtatgtccac aaatatttcc gttgttgaac
    13141 aattgcgatg gcgaaagctg cccgtgctgg acgacggctt tgtgtgcctg gtggatgtca
    13201 tgggcgatga ctcagcgatt gtccaggcgg cccgtgttag ctacggcgcg gggaccaaga
    13261 aagtttccga cgatcggacg ttgatccgct atttgatgcg tcaccgccac acgaccccgt
    13321 tcgaaatggc cgaggtcaag ctccttgtgc gggtacctat ggacatttgg cgtcagtgga
    13381 tccgccatcg cacggcctct gtgaatgaat acagcacacg gtattcgatc gccatcgata
    13441 ccgcccacag aacgcctccg gataagtggc gaaagcagtc cacaaccaac cgtcagggca
    13501 gcgaagggtt tttggaccta caaacgggag aagagcttag ccgcgaggaa agagagttgc
    13561 aggaaaaggc ccggcaggtc taccaacatc gcctcgattt aggagtcgca cgggaacagg
    13621 caaggaaaga cttgccgctt tcgacctata ccgaggctta ctggaagatc gatctgcaca
    13681 atctgctcca ctttcttgaa ttgcggatgg atcgtcacgc gcaatgggaa atcgcccaat
    13741 atgcgcgtgt gatcggctac gaaatcgtcg ctccgctatt cccgatgaca tgggaagcat
    13801 ttgtggacta tagattgcaa gggctggtgc tgtcgcgtct ggactgcgag gtcatcactc
    13861 ggcttgtggc tcgtgggtct ctgccagcga ccgagaagga ctttctcgaa gctcaggacc
    13921 cagcctggcg gggactaaca cgttgccgcg agcgagacga gtgttgggaa aaactcaaac
    13981 gcctagggat cgtgaaggat ccatagccta taaactcgac gaagaagtta caaaagaggg
    14041 tctctgatgg ccctgctgtg gcattgcccc atccctggca ctgcccgatc actagtccgc
    14101 agtagcgtga tatattaaat tctcatcttt ctcccgccat tggcagataa tgtaactttc
    14161 cgccggagtt actcggccaa cgtattgccc cctggaccat ctgcaaagaa ttgccataat
    14221 agtgaagcta catgaatgtg gtccagtgat agaggccggg cgcggtgttg ccctgacgtt
    14281 tgctgcctag ttggccgttc ctctgtgtgt atccgaggaa aacgctcccg accggtgtga
    14341 attcgtctct gatgatccga ttgtcacagc aaccactggc cggaacggaa taatgatcag
    14401 aaggagtgtt ccgcaatgcg taggagagcg acaaatcatc acttacatgg agcgtttgtt
    14461 ttttgtttgt tggggtggct tatgtcgtta ggctcgatgg gggtggcagt ggcaaggaac
    14521 gtggaggagg attcctcggg agaagggttg ccgactattg tcaagcggct ggagtttccg
    14581 gacgcggtct ccctcgttga actctcgaac ggcttgacga tcattgttca ggaaaatcat
    14641 tcggtcccgg tggcgaccgt tcgctgttat gtgaaataca ctggcagcat ttacgaagcg
    14701 gaatatctcg gtgctggtct tagtcacgtc ttggagcatg ttgtctctgg tgggacaacc
    14761 accaagcgca ccgagcgcga aattgctgcc atgatcgacc gttttggcgg caagagcaac
    14821 gcctacacct caacaaattt tacgtgttat tacatcgact gcccatctaa aaacgtcaac
    14881 gaggcgatcg agctgatagc tgacgccatg cagcgcgtgg cgtttgagcc ttccgaattc
    14941 gaacgtgagc ttaaggtggt taagcaggaa cttgccgacg gcgaggtcaa tcggtctcgt
    15001 gtgttgtgga aactcttgaa tcaaaccttg tacctccagc atcccgcccg acatcccgtt
    15061 atcggttatc tcgatgtgtt aaaccgaacg agcaacgagg cgatcatcaa tttttataag
    15121 tctcgttacg ttcctaacaa tcaaattttc gtggttgtcg gcgacgttac ggttgacgaa
    15181 attgtgaaaa gtatcgccaa gcagtaccag gggagtaagc gagcccccga gacgtacctg
    15241 ccacttcaag aggaaccacc acaattgacg ccgcggcggg tagtgcgcga gatggaaggt
    15301 gatgtcgttg acttcgtgat tgcctggccg acggtgaaac tctccgatcc cgatctgtat
    15361 ccgctcgatg ttgcggcgga tttgctatct cggggtgatt cgtcgcgatt ggttcggcgg
    15421 cttcagcgag agcaaccgct cgctttgcag gtcgatgcag tgagtgcaac cccggaatat
    15481 gttcgggggt ggtttggtgt tatggctcgc tgcctgcccg atcgttttga ggaagtccgc
    15541 aacatcattt tccaggagat tacccggcta cgcgatgaac ctgtttcgga agccgagctg
    15601 gaacgggcca aaaagaagaa gatttccgat tgggtttttg aacgcgaaag tgtccaggcc
    15661 agtgccgaaa gtttggcccg tggcttcatc agtaccggcg atcccctttt tgacaagcaa
    15721 tatacagagc gaatccaagc ggttacggcg gagcagatta gggaagttgc gcgaaagtat
    15781 tttcgcccag aatggctaaa cgaggtcatc attgccccgt tgggtcgatc tcccgcaacg
    15841 gtgaaggcgg atcaaacaag cgacgaaacg gccgccaggt tggtccgtct caacaacggc
    15901 ctgcgggtag ttgttaagcg gcagccacgg cttccgattg tgaccatgca aattgccatc
    15961 cttgggggaa atctcgtgga cacgccggaa actgccggtc gatcttcgct gctagctatg
    16021 atgctcgaca aaggcacaga gcggttgacg gcagagcagg tggcggagat gtttgatgat
    16081 cttgatgcac gggtcagttt tacacccggt cgcaatacgt tgctagtagg gttgtcgatc
    16141 ctcaaggatt ctttcacacc ggcagcagag cttttggcgg aatgtctact aaagccccgc
    16201 ttttccgagg atgaattcgc gaaagtgaaa gtcctagccc ttgacgcgat cgctcagcgg
    16261 acggccaatc cgcaggcaga ggctcaggag ttgtttgccg acctcttgcc ggaggaatcg
    16321 ccttaccacg ttgtcatggg cggcaaaaag gaaactgtag aacgaataac cataaacgac
    16381 ctcaagaacc ttcacgcaca gatcctcaat ccggaacaga tggtggttgc cgtctacggc
    16441 gattgtgatc cagagagggc tgtggaactg gtggaccggc tctttggtgg ccttcgtccg
    16501 cccgccaatg caccgaggat tagtttcgat cgaagcaata agattgcccg catcatttct
    16561 gggcacaagc aaatccaaaa gcccacgagt gttattgtaa tgggctatcc ctgtccaggt
    16621 ttccacgacg aaaaggacta cctggcgatg atggtccttg acacaattat gtcgggctat
    16681 gactatccgg gtggctggct acatgaggaa ctacgagggg aaggattagt gtatggggtc
    16741 catgccctac agctgaccgg gcccgtccca ggttattttg tgatcattgc tcaaacgagg
    16801 ccagaggccc aaaacgaggt catcacgcgg attcttaaca atgtcgataa ggcaaaacgc
    16861 ggacagatca gcgaggaaga gttcttacgg gccaaagagc ttttagtggc ctatcatgcc
    16921 cagaaaaaca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagagttct
    17041 tacgggccaa agagctttta gtggcctatc atgcccagaa aaacacgacc atcgaagaac
    17101 aggcccaaca ggccatcctc gacgagttgt acgggcttgg tttcgattac gaccgggggt
    17161 tcgagactcg catccaggcg atcacactcg acgatgtcaa aagggttgca cgggagtatt
    17221 tcggcaatta cgtccttgcc acggtctcgc ctctgaagtc tctcccggaa gaagcctcaa
    17281 cgggtggcga tcgttgatcg caaaaaaagg agcaaccgtg ggatgaggcg ggatgccttc
    17341 ccacggttgc cgaaaacggc cattcgcccg ggttgtcaga atccgtattt ggccatcagc
    17401 tccaagcgat aactgtcacc gggttccttg ttaacccagt tcgtcttcca ttgctgaact
    17461 tctataccca ggacaaattt cttagtgaca tcgtaggtta ggttgacata gtaaaattgg
    17521 ttgtagctcc gttgcccggg actaaggtcc ccattgagcg ggtcgtcgat ggaataccca
    17581 gcgtgggtat ggagtcggct ggtccagtcg taccagatgt cgaaccatcc accggtggaa
    17641 cggatggtgt ctcgttgcgt caggttaatt ccctgaacga tcccacccaa gtaggcgcca
    17701 aggttttcgc cggtaaagaa ttcgccctgg acaccaaagc gggaggtgat tggccatttg
    17761 gcgtccacgt tgaacgacca agtgcggaac tcaacgtcat cctgtacagg tgctgcgaga
    17821 aaatcataaa cctgttcgcc gatatggctg gaaataccga tttccacggg acggcaccct
    17881 ttgcctcgcg ggccgagcgt gaaggccagt cgtccctcga ccacaggcca gtctgcgtgg
    17941 tccccgataa cgttaccggc gaactcggaa acgacatcgc tattgatcga ggcctgggtg
    18001 gttactaaca gggtatcaga aaagtggaga tagcgttctg cccgaaattg agcacgccgg
    18061 tacccaaggt tacccgctcc ccaaccgacc gagtacatga tcgtccccgg cagtagaggc
    18121 gagatcacgt cccaagtttg cccggcgagc aaacgaaagt actcgttctt gacttcaaga
    18181 taggcgtgcc gtaaaaggac cgtgcccttg ttttccgtct gatactgacc ctgaaaatcg
    18241 atttccacct ttcctcctgt ctgagcctct ccaaagttgg gcaacacggg accaagaaag
    18301 tcgattccca accgtgtgga tttggcatcc acatgccatg cggactcacc ttggtcggtg
    18361 ttcgaaagta cgtaaagcac atagtcccca acctgtgatc gagaagattc gtaagccgtc
    18421 gtggcccaaa gatacccata aggaatgatc tgaatatcgc ctttggtcca cgcgattttt
    18481 ttcatctccg cacgaatctc gtccatactc ggggtctgcg ccgcatcttg cggagccccg
    18541 acaaagcccg tttggtcaac ttgaataggc gggaaggcaa caggccgcga ttcttgaagg
    18601 gattcgagct ccgcccgcaa ccgttgcgtt tctaactcca attggcgcag tcgctcctcg
    18661 gtcagtccct gtgaaaaaca cggcggcgca agtagcccaa aggcaaaaac caacatgatc
    18721 cagacacttg ttttcatcac ttcactccaa ttcctaggag atgcagaggt ttaatggcca
    18781 agaatactac tttatagttc cacaacagcg cccggccggg gtaacctttt ttctggtcaa
    18841 tcagcgctct ccactctcta gcgtccggac agggcactat ctcacgttgt ccggccaagg
    18901 gaaacgtcat gcgctgttga cattttcagc actagtccga aaggtcttat ccgcaatagg
    18961 tctgacagaa ccggtataac gctatttgct ctcatcgtca gcatgttagg aatctaatta
    19021 agagccatcg gcaaggggtt gcaaaaaagc acatcgagcc tcgccaggag gttccagatg
    19081 tatcgccttt actgagattg cggagaggag accaccagtc gcttgggttg acgcggccga
    19141 ttgccaggac atggcaaggg tttttgggtt gcagttacgg ggcagcgatg cgcggtaatg
    19201 ctataacgaa cgtgaaagct accgccgact ttgccccggg tttctcggga ggaaggttac
    19261 gcagcttgtt tcttactgtc cggcaggatt tgtggattct gctgaatagg cgaacttttc
    19321 tgattttgct gaatttccca gagcttcacg tatttgaggt atgtcgaaaa ggcaccatcg
    19381 aatgcgacaa taagacctgg tattccgtca agaaagccca gtcggaaaat ataacccgcg
    19441 ataaattgaa tcaatggtcg taagaacagg tcgtacgctc ttgcccttcg tccgcgcttt
    19501 gccagttcat tcgctccata cctagcgtaa gcgtactttt tctggaacca gcgatcgagc
    19561 gaagtgcacg taaagtgatc taaccgagaa tggagtttcc gcacgcggcc actgggcacg
    19621 atgaccttct cgtgaacttc ccggctatcg taatggcacc gggattggcg aaaaagcctt
    19681 atgacacccc taccgttcca tttgccaaag cgaatccgat gtcccagaaa atagtgccta
    19741 aaccgcacga ggtaggcatc cgctgccgac gaagaggata agacggtgtc tatttcatgc
    19801 gagagcgact tgctcatccg ctcatctgcg tcgaggacaa agacccaatc gtgacggcat
    19861 tgttgacttg cccagctttt gaaactacca aaattgatga actcacgctg aacgagtcgg
    19921 cacctcgtcg gaaacctttc gcataacctc gctgcaattt ccaatgtgcg gtccgtggaa
    19981 ccactgtcgg caataacgac ctcgtcggcc caatccacaa cagacaagac acaatcttcc
    20041 aggtgatggt cttcgttttt gcaggggata acgacactga gttcgggtcg aggcatgact
    20101 tacagatcct ctttctgagt atcaggctgc cccccgcata ttctgctgaa taagaaaagg
    20161 ttggaggtgc tcaaacacca gctcgggcga aaggcccttc atgcagcggt gatgcccctt
    20221 cggacacgtt cgacagctac agggagcgca atcaataggg atttgaagat cgatgctttg
    20281 cacggtgggg ttggcagtga caatgggtga ggttggtcca aagagtgtga tgaccggttt
    20341 tccaaatgcc gcagccacgt gcctgggacc gctgtcggtt gaaaccatca catggcaccg
    20401 ggccagacat gccttaagtg tctgaaggtc cagcggatga tccgccatcg aaactactcg
    20461 tctgtgattc acctcgcgtg caattctttg cacggttgcg cgttcccgct gaccacagac
    20521 gaagagtaca gaggccgagg tgcgggtcgc aatcatccgg gccaattcga caaagtgcga
    20581 gaatggccag gatttggctg caccgtaggc cccagcgaca tgcatcgcca caacttggtc
    20641 agaggaatcg agtgaaagat cccgccatat ccggtctgca cataccgttc cccagggcga
    20701 caatggaagt tcaagccgcc tgtcctgaga agggaacccg gccactcgcg ccagggccag
    20761 gtagtagtcc accatcggga tgtagcgccc cgtaccatgg acttcgtgga cactcaccgg
    20821 atgggtcagc aggaggcgac gcccatgacg ggagtaccca acccgcttgg gaattccgcc
    20881 agcgacggcc aggagggccg agcggaagct attggtaaaa aggatcccca cgtcccagtg
    20941 gacagaccgg agtttttcga tgagagtggc ggtttcaaac cgtggatcgt cgccacgcgg
    21001 atcgaagtac cattcttcgt cgagccactg acttccggcc agtaaacctg ccaggttcgg
    21061 tcgtgtaata ccgacaacgt atgccgacga tccggttgcc tcacgaatgg ctcgcagcgc
    21121 cggaacggcc atcaccagat cccccaacca gttggggagg aagatgcaga tattcatcga
    21181 atcggcctca cggtcaatta ggggcaggct gctgccctca gtctcgtcat caatcgccct
    21241 ttaggtggca ttttggccaa gattccgatt ttggaaaaga ccaatttgga agagcggtct
    21301 acgccatcca ggaaaacgag tcgaggtacg ccgtccttct atagtgctct tagttttcgg
    21361 tcccggctgc gagaagactt ttggctgctt aaaaggcaaa agaatcgttg caaaatgaag
    21421 aagcctgccg cgcggtcctg caggtctcgg ccgcggggag gcggacggtc gaaaccggcg
    21481 acacacgcaa caggcctcaa aaaataatat acaaaacgga taacgtcgct tccagacctc
    21541 ttcggaaacg ctcatttgcg tcatttgtaa ttggcacaaa tcgcccccgc tctagtggta
    21601 gtccgctgtc tccttctttt tgggctagaa ttgagtaacc catattgctc cgaacttccc
    21661 tctcgtggct tgagggagtg atgtgaggga tgttggggaa cccgatcgag aatttatcac
    21721 gaggccggaa agccaggcgt taacagccaa ccttttggcg aagggtcgtt agagtggcaa
    21781 caaaggatgt gctatgcggg ccgagttcat caatcctttt atccattcgc tttacaaaac
    21841 attttccacg atgctcaatt gctcggtgca acggggacag ttaagcctca aaaccagttc
    21901 aaaaacctct tacgagatca gtggagttat cggccttacg gggcgcgcag tgggggctgt
    21961 ggtattaact ctttccaagc cggtcgcctt aaaagccgcg tcaacgatgc tcctctgcga
    22021 cttttccgaa attaatgagg atgtcatcga cgccgtcggg gaactggcca atatggttgc
    22081 cggtgcggca aaggcagagc ttgaagaata tgccctcgtt gtaagtttgc ccaacgttgt
    22141 tacgggcaaa gatcatgaga ttcattttcc ttcgaatgtg acgccgattt gcatccactt
    22201 ttcttcggat tgggggcccc tcgcactaga ggtaggactg gcaacgatcc aaaacccctc
    22261 gccagaagcc gcgactttga cccatcattc cgaagcagtt gccgcttctt aatgagcact
    22321 tcacaaagat aattccttca tcgcgaaccg atctgttctt tactccagaa aagtggctca
    22381 aagcccaagc caattccatc gaggacgtgc gtacccaccg ttccatcggt gatccggaac
    22441 atagtcggat atttttcgtc ccaacctcga ttggcggagg gacaatactg acgggcgttt
    22501 ccctcgatgg ccgcaacccc gggaatgcgg gcagccagcg acgcggaatg caagaaggaa
    22561 taaccagggc aggtcaaatc ttgcacacac agaaacagcc caaacttctg tgccgctgcc
    22621 gccatgagga gggcctcgct gtggcctttg cacgttttta gggccacacc cgagtatcca
    22681 agttcgcgac ttaataacag gctttcgaaa tcgaccaaag attcatcaat gaccaccggc
    22741 ttcaaagccg ccgccttatg catccggttt tccggaaatc tctttagatc acggtgcgta
    22801 ggctgttcta ggtattggag tcggtcgaac gcctcgggcg aattctcgcg gattcttgtc
    22861 atgaactcca agacgtagtc cacgctttcg catcgctcat tgaaatcggc ggagtaatgc
    22921 caagtgtcgc agccgcgagc tctttgggcc tcgctcgtta ctcggtgaat ggccagaaca
    22981 cgatcaacgt cccaccgcaa atcgtcgcca ttgagcttga tctttaggtg tgtgagacca
    23041 ttcgcataga tccactctaa aagcgtttcc ggcaacccat cgttgagacg ttccgacagg
    23101 tcttgatcgg tcagaggatc aagtgccccc acaaggtggt agaggggcat tttcgctttt
    23161 ggctgccgat acgtgtaagt atcgagatac tcacccttga actccggcga gagaaagtgg
    23221 gccaaatcgg cattaacgaa ttcggaactc agcgtgttgt aggcgttgag gttcaaagcc
    23281 cggccatatg catcatggag tgcagcttca aaggggctgg ctgccacaag ttgggccagc
    23341 tttggcattg gttctgaaag agattgttcc gcctccacgc tggcggccaa cttgccgtat
    23401 tccgtagccc aatcctcagt gatttcgaga ggatgccccc ggccttggta gtcaccgaca
    23461 accttgacca ttttttcgcc gaagaggagc atggctccca gggtcatctc tgcgctaaga
    23521 acttggctgg gccacgccca tacatttccc acgggcatcg agccgaaacc tcgcccacgc
    23581 cgtccggtgc gcgtttccac ctctacaacc acattcctta ggacaacgtc cctgacaacc
    23641 cgcccaccaa acttcatggg ggtccggtag ctgtattctt ccgtccagga attgacgcca
    23701 acaattcgaa tgtcggatgc gcggcccatc tcacgtgtct cctctgtgat tgtcgagtcc
    23761 tctcgcagcg gctaaacggt taataccaca aaaacggccc cttcgtatct ccttgaatga
    23821 ttctgcaaaa cgaggctgtc gtccacaagg gtcaagaaca cgctgcccaa ggacaagggc
    23881 cgcattttgg tggagtttga cgaatttttg acagtgacta ttgttgctcg tgaccgaagc
    23941 ttaagtgatt tgacgggctt cggcaactcc tcccccgcac aagtaacgaa cgtgatctga
    24001 tatgatgagg tatgttgcca ataggaacgg atatgtcgct tgttcatgca acatgcaggg
    24061 ggagagtcac tttcatgccc gacgacgcca aaccagctgg tgttttctcg gcgccatgtg
    24121 cgtgccagga agtctcggaa gaggacaggt acgcgcagct tgacgatatc ttgcgaacct
    24181 accgcaacat tcccggggca ctcattccgg ttcttcagat cacacagacc atctttggct
    24241 acctgcctga gaaggccctt cgcaaaatcg cggccgcgct aaacaagccg ctcagtgagg
    24301 tcacgggcgt tgtgaccttt tactccttct tcaatacgat ccctcgagga cgtcacatga
    24361 tccgtgtctg ccttggtacg gcgtgttacg tccgtggggg taagcaggtt ctggcggccc
    24421 tcaaagaaga gctgggaatt gatgtgggtg agacgacagc ggaccggaac ttctcgctcg
    24481 atgttggacg ctgtttcggc gcttgtggcc tcgcgcccgt cattatgatt gatgacgatg
    24541 tccaccagcg ggtcaaagtc tcgcagcttc cccgccttct tgagcaatat cgcgataaac
    24601 aggatacgac atcgaagact tgaatgtgct tcggtgttgc gaaggttggc aggtcttgct
    24661 tccaccagtt gccttctttt ggagtgatga catttgtttt tggagtcaag cagtgaccat
    24721 ggctgaagaa tctccaaagc gaaaccgggt gtccagcgtc aacgatttaa atgcgtggcg
    24781 caaccagttg ctcgctgagc agaaaaggca gtcagacctt gggattaagg agttgcgaat
    24841 ctgcaccgga gcaggctgca tagcctccgg ctctctgcac acgcgcgagg aaatcgtacg
    24901 acagttgcag gaccgagact tggcaaatcg ctggaaggtt ataagcacgg gttgtatggg
    24961 cccctgtttt ggcggtccag ttgtgatgat cggcgatgtg gtttacgagc gcgttcgcgc
    25021 cgaggatgcc gccacgattg tggtggaaca tcttgggcag gggaagattg tggaccgatt
    25081 gacccaccgt cgggcggacg gtcgccacgt agcgcatctt aaagacatcg attttttcaa
    25141 acggcaggtc aaaatcgccc tccgcaattg tggcgtgatc gaccctggat gcatcgagga
    25201 ctacattgcc cgcgatggat atcaggcctt ggctgaggta cttgaaaaaa gagacccgga
    25261 tgctgtcatt gagacccttc gtgaatcggg gttgaggggg cgtggcgggg ccggatttcc
    25321 aacctggcga aaatgggcat ttgctcggca gcaagaggcg cccaccaagt tcgtggtctg
    25381 taacgccgat gagggagacc ccggggcgtt tatggatcgg agtatccttg agggagaccc
    25441 tcatagtgtg atcgaaggaa tggccattgc ggcggccaca atcggtgcgc aaaaggggtt
    25501 tatctatgtc agggcggaat atcctctggc ggttcagcgg gtgcaggtgg ccttgtccca
    25561 ggctcgagag gttggtcttt tgggggagaa tatcctgggt ctgggttttg attttgatat
    25621 cgaaattcgc atgggttcgg gggcatttgt ctgtggcgaa gagactgccc tgatgacttc
    25681 gatcgaaggt aaccgtggcg agccaaggcc gaggccccca tacccggtcg aaaaaggact
    25741 ctggggccac cccacgctaa ttaacaatgt tgaaaccctg gccaatattc ccatcattat
    25801 cttgaaaggg ggagcatggt acgcccagtg gggtacgcct aaaagccgcg gcacgaaggt
    25861 ctttgccctg gctggagggg tcaagcacgc cggtttggtc gagatcccta tcggtatgcc
    25921 gctgggcgac ttgatctacg acattggggg cgggacggca tccggaaaac cgttcaaggc
    25981 ggctcaaatt ggtggtcctt caggcggatg tatccctcgc caacatctca acgttcctct
    26041 cgactatgaa tcgcttgccg agctcggtgc cattatgggc tcgggaggtt tgatcgtgat
    26101 ggatgacgat agctgcatgg tggatgtcgc ccgctttttc ttggagtttg tccaggaaga
    26161 atcttgcggc aagtgtgtgc cgtgtcgcgt tggcacaaag cggatgctcg agatcatgga
    26221 aaggatatgt gccggccagg gtgaagagga tgacgtcgaa cgccttatcg agttagggga
    26281 gatgatcaag gagacttccc tttgcggctt gggacagacg gcttcgaacc ccgtcctttc
    26341 cacaattcgc cattttgggc aggaatacgt cgaacatatc cgcgaccgcc attgtcgggc
    26401 gggtgtgtgt tccgccatgg tggcggcacc gtgcgaaagc gcttgccctg ccaaggtgga
    26461 tattccaggc ttcgtggccc tggtggctga aaaacgctac gctgaagcga ttcggttaca
    26521 ccgcgagcga aatccctttg cggccgtctg ttctcgagtt tgcttccaca cctgcgagga
    26581 caaatgccgt cgtgccacga tcgacgctcc cgttgccatc cgtgccatca aacggttcat
    26641 ggtggatcaa gagatcaccc ttcaggtccc agaaatccga gaagatccca agaatgccaa
    26701 acgaaaaatc gctatcatcg gtgctggtcc ggcgggtttg acgtgcgctt attttctggc
    26761 ccgtctggga tatcgtccgg aagtcttcga ggctgagcaa cgcccgggtg gtatgcttgt
    26821 tcaagccatt cccgcttatc gacttcctcg tgaggttgtc gctcgtgagg tgcggatgat
    26881 cgagcagatg ggcgtcgaga ttatcacggg catcgccctt ggccgtgact tcacgttgac
    26941 ggaactccgt gcccagggat acgaagcggt ctttcttgga gtaggggcgc ccgaaggcac
    27001 gcggttaggt attcccggtg aagatgtcca aggtgtcgaa gacgcattga cgttcctccg
    27061 cacatacaac attcgagggt cggtacgcgt tggaaagcga gtggtcgttg tgggtggcgg
    27121 caactccgca attgatgctg cccgcacggc cattcggctt ggggctgaga tggtgaccgt
    27181 ggtttaccgc cgtaatcgtg aagcgatgcc cgcctatgaa gaagaaatca ccgaggcaga
    27241 ggcggaaggc gtgcgtcttc agctccttac cgctcctgtg gaggtcctta gcgagggagg
    27301 gcaggtcgtt ggactaaaat gcgttcccgt tcaattagga gagtttgacc gatccggaag
    27361 acgtcggccc aaagaggtcg gcgaggcgtt ttgtatcccc gctgatcaga tcttggtcgc
    27421 agttggtcaa acgctcgatt tggagcggct tgtccccgac gtggaactgc gccgtaattc
    27481 gcgtgggttt attgaggtcg atcccgtcac gggtcaaaca tcccatcgct ggatttttgc
    27541 cggaggtgat gccgtcaacg gtccgtcctc ggtggttgaa gccgtggcgg ctggggagcg
    27601 agccgccgtt ggcattgaca tgttgctaac agggcagtcg cacgcctttt ggcgagagac
    27661 aaaacctgtg gaaactagct ttgaccctga tgcagagcct gaggcgactc cccgcgagcc
    27721 gctgcgcctc cttcccgtcg agcgacggcg ccacaacttt gacgaggttg agctaccgtg
    27781 gccagagccg gttgccctcc gtcaggcacg acgctgctta aggtgtgatt atggaaagcg
    27841 gaatggcgtg caaggacccg ttctccatca ggttggcgag ctgtgaatca ggtaccgtgc
    27901 cttgcaataa gcacgcaaag aagacactcg ccaagccaag gatccacgat gagtaaggtg
    27961 ttgatatggt aaggatttac attaacgatc aagaatatga cgttcctgcc ggaacgacca
    28021 tcttgtgcgc tgcaaagaag gccggcattc gtattcccac cttgtgcttc gtagaaggtc
    28081 tgcgacccat cggggcctgc cgtgtgtgcg ttgttgaggt ggagggggtt cgcaacctag
    28141 tcgcttcgtg ttccacacct gttgccgaag atatgcgtat tcatacgcac acggcgaggg
    28201 ttcgtcgagc acgaagggcc gtcgtggagt tgttactttc ggagcacaac ggcgagtgcc
    28261 agacctgtga acgaaacgga caatgtgaac tccagtctct cgcggcggaa ttggggatca
    28321 gagaagttag ctatcgaggc gagaaggccc gcaagagaat tgacatctca acgccggctt
    28381 tgcaacggga tagcggcaag tgtatcaagt gccgccgatg cgtaagcgta tgcaatgaga
    28441 tgcagcacgt gggtgcccta tttccccagt ttcgcggtta tcagacaatt attggaccag
    28501 cgctgggacg tgaccttgct agcgttccct gcgtgcagtg tgggcaatgc gcggccattt
    28561 gtcctgtggg agcgatttcc gagcgcgacc acgtggaggc cgtttgggcg gcactcgaca
    28621 cgcctgaaaa tcacgtggtg gtacaaacgg ccccggccat ccgggcagcg ttgggcgaat
    28681 gctttggcta tccgccgggg acgttggtca caggaaagat ggtggcggcc ctgcggcggt
    28741 taggttttga tgccgtgttc gatacaaact ttgcggctga cctgaccatc atggaggaag
    28801 gtacggagtt cttgatgcgt cttcggaaag cctatgtgga ggggcaacag gacgtccttc
    28861 ccatgttcac aagctgttct ccgggatggg ttcagtactt agagtttttc catcataatt
    28921 tgctccccaa tctttccacc tgtcggtctc ctcagcagat gttcggggca attgccaaga
    28981 cttactacgc gaaaaagctc gataaacgcc cggaagatat cttcgtcgtc tccgttatgc
    29041 cgtgcacggc caagaaattc gaagcgtcac gtgcggagat gaatgccagc ggcgttcgtg
    29101 atgtggatgc cgttctcacg acacgcgagt tggcccgcat gatccgaatc gccgggatcg
    29161 attttcagag cttaccggat gaggaaatgg acgccccttt gggactgtgt accggtgccg
    29221 gcgtgatctt cgggcacaca gggggagtga tggaagcggc cttacgcacg gtttacgaag
    29281 ttctcaccgg cgaagatcta ccgacaccaa cgctgctggc tacgttggat ggcatcaaag
    29341 aggcggagat cactcttaag aatgtccgcc ccgagtggtc attcctcgag ggtgttacct
    29401 tacgggttgc ggtcgcccat gggctgggga acgcggaaaa gcttatcagg gccattgagg
    29461 ctggtgaacg tcaatatcac ttcgtggagg tgatggcctg cccgggcggc tgtattggcg
    29521 gtggtggaca acccagattc acggataacc gtgtcaggtt ggcccgaatt gcagccatcc
    29581 gcaaagagga cgcggggaaa ccgcttcgaa aatcccatca gaacccacag gtcaaacagt
    29641 tatacgagga gtttctgggc gagccgctca gcgaacggtc ccacaaactc ctgcatacgc
    29701 actacatcgc tcgtgagcct gtctaaccca gcgcggtgat ccccgtggcg cagcactgtc
    29761 aagacgctca ttttctgccg aagtggcgga aaacaggcga ttccgcccgg gattaccacg
    29821 gcccacgaat gtaatacatg ccgaactggt cggtgtcggt tggccactgc ggcgtgggct
    29881 ttaaggggag ataaccccct tgggcgggtc ccggccagtt tcggctaaac tggtggtcga
    29941 tgggggtgac acgaaaattt ccaacacccc agccccagtg ggtttgaaac tcggccgttg
    30001 gcgggacaac cacagcaatg ggggctcccc atgtggcgcc gtaatactcg ccactccatg
    30061 agtaacttcg agcccgctgg gctgcccgcc tgccgaaaaa ggtgttttcc ccagccgagg
    30121 caaccagagg cacggagacg gtcaggataa ctacacacac actgctaatc agaaatcgtt
    30181 gacgcatagg tacgtcctca cgtgaatcag tggcgttttc tttttcaacg atcaatctgg
    30241 gatttagtac cacccaacga caacccttgt ccagccacct tgggggttct tacgccagta
    30301 aaccttgctg tgtttgtaca agaattcatg gggatacagt ggctgatacg ttacccaaac
    30361 atgtccgacg tgcggtgggg tggggatcgg agatacgtag aggggggccg aaatcccccc
    30421 aggggctgct ggcgcccaat agctcggaaa gagatccccg gcatgagagc cctggtatct
    30481 ccccatgaca aagctaccat cgcccgccat gcttggagag gtacttgccc agctaacaac
    30541 cgcagcaaag aaaaacaagc cgattttcca gcctagcaat ttccggatct gcatatccgt
    30601 catccttttg caaataaatc ctcttccgtc caacccgcga ctatgcgcgc agataggtga
    30661 tgcaacaatc tcctttatca actttcggct acccttgacg acgagggtga gtctttataa
    30721 aggcgaagta agaagcaagc tttgcatagg cgatctacgt cgtttgatct gtaggaatct
    30781 tcggggataa tcgctacaat cgttacattc gtttcgttct tcccctaaaa aagtcgcaac
    30841 agataggagc tgttttgcct tggatatggt gaagtccacg gaagagatgt ctttggaaac
    30901 gagcccagaa aaacgatctg gtccccaaaa tggtaacgga caaactcgct ctccccggat
    30961 tcgcgtgacg gccgaatcga tggccgccaa gcaacgcgaa atttcggtga gtgagttctt
    31021 tgccaagaat cgccacttgc tgggcttcga taatccccgc aaggcccttc tcacgatggt
    31081 taaggaggcg gtcgataatg cccttgacgc ctgcgaagag gccggcgtct tgccagagat
    31141 ttgggtgcga attgacccca cgggcgagaa ccgttatcgc gtcattgtgc aggacaatgg
    31201 accgggcatc gttaagaaac aaattcccct gatcttcggc aaattacttt acgggtcgaa
    31261 gttccatcgt ctccggcaaa gtcgcggaca gcaaggcatc gggatcagtg ccgccggaat
    31321 gtatggacta ttgaccacag gtaagcccgt cagcattgtc tcgaagacag gcccgaaaaa
    31381 gcctgcccat ttttaccaaa ttcaaattga cacaaagaac aacaggccgg agattgtcaa
    31441 cggccggggt gagggtatcg acatcccacc tcaagaggat attggtcaat tccttgttcc
    31501 gtacggcctt ccttggtcgg atgtacctca tggaacactg gttgccattg agattgaggc
    31561 aaagtttcaa cgcggtcggg gcagcgtgga tgagtacctt taccagacgg ccattgccaa
    31621 tcctcatgtc accattcact atgtcgatcc cgagggcaaa tctcgcgact tttcacgggt
    31681 ggctgacgtc ttacctccgg agccgaagga aatcaaaccg catccttatg gtgtggagct
    31741 tggttggctg ttaaccatgc tcaaggacac gaaggccagc acgttgagtg ggtttctcgt
    31801 ggattccttt tcccggatca gtccggctgt agcccagcgg atcgccgaaa cggcccaatt
    31861 gagtacgcgg gccaatcccc gcaaaatcag tcggcacgac gccgaggccc tctttcgcgc
    31921 ccttcaggag acaaagattc agtcgcccgc taccgactgc ctttccccga ttggcgaaga
    31981 gttgatcctc aaaggtctga gggaattggt ccctggcgaa ttctacacgg cgatcacacg
    32041 accgccggcg gtatatcgcg gcaatccctt tcaggtggaa gtgggcctgg cctacggcgg
    32101 ggcaccggcg acctatcggg tttcactgga agttcttgaa gaactcttgg ccgaaaccga
    32161 cgcgcgaaca cttcgccagt tcctgacaac cacatttgac ggcatcggac cagagggcgc
    32221 tgataggatc atccatcaag cacgtctcaa atcgaggatg tcgccggcaa agctcaaggc
    32281 tagggaaagg gaacagcttt gccaggcttt gcaaacagtg aacttgagtg aaggccagac
    32341 gatgactatc ctccggtacg cgaacagggt gcctttgcta ttccaggcgg gggcgtgcgc
    32401 gatcacacag acagtcatga gcaccaactg gcggccgtat ggtttaacac aatcgcggaa
    32461 tgccttgcct tctgggcctg tcacgctcat ggtccacttc gcgagtgtgt gggtgccctt
    32521 cactagcgaa tcgaaagaag ccatcgcggc ttatcccgag attcagaggg agttgcgact
    32581 tgcctatcaa gctgtgggac gtcgcctcgg cctatacttg agacgtcgtc ttcgcgtaaa
    32641 gcagcaggga gagcgtcgaa atatctttct ccgctatttg aaggaggtgg caaatgctgt
    32701 ggccgtgatc cgaaacgtgg accgcgagtc cgtttatcgg gatctgatgg aagtggccaa
    32761 acgcgtcacc gctgaagcag atcttaagtt ggatgaacac ggcaatccga ttgaagaaac
    32821 tccatcctcg gaggagtttg atggggccgt gataattgtc gaacagcaac agggtaaccc
    32881 gccgttacga tccgggaatt cgaagcccat tcaactccag ctttttgagg acaacgggga
    32941 aaatggcgaa ccgtcgcccc aggacgagga agcgacgtag agactatctc aaaaagtcgc
    33001 cctctccctc cgggagaggg ttggggtgag ggtaaacgaa catcggaatc gattgacaaa
    33061 agcggcagca atttgagcca ccctcacccc cgacccgtct cccgccgaac gggcgagggg
    33121 agattatcgg ataggctcgt agtcaaaacg tcaagaacct gtcagatcct catcccattc
    33181 aggcggcgac tgttcgctga tggcctccaa aaacgggtcg caagggtggt ttcgggttac
    33241 gtgctgaacg gcctcgggga agtcccaatt cattgcccaa tggagatagg caatgatgac
    33301 ggtgggcgat cggttcattc cggccgtaca gtgaacgtat acccgatgtc cttctttgac
    33361 aaggttgtgc aacagtctga cggcttctgg taatcgtcgc cgtaggtctt cataatcaaa
    33421 atcgcggata ggatacctga tctcgcgcaa gcggtaagct cggtatgctg cggagacacc
    33481 gtctcggtca atgttgcgcc aggcgatatc ttcatccgtt tgaaggttca atacggccgt
    33541 gatgccgagt tccttcaact cctgaacgtc cgtctgcgag gtggggcaag aacctacgta
    33601 aaggcaatcc agaatctttg tcacgtgcat gatgaatccg tatttgagga cgacatacat
    33661 ctcctgtagc tggggcacag tcttgttaag ctacacccaa gctacttcaa tatatcactg
    33721 acttaacaca acctattcca aaagcagccc ggatactgtc aaagggccct tctacacgtt
    33781 atcggggatt acataggggg gacggtattc tcgtcggtaa agcgccgctg cttctggaac
    33841 attggtgatt tgttccgctt tggggtcaat tacgagctta actcctccca gccgataact
    33901 gatgttcgcg aagtgaatcc acagggcact acgatgtcct tcctccacat cggcgttggg
    33961 cagttttcga gtgcgtatgc aatcaacgaa gttagcctta tgttccgggt cggggaagcg
    34021 accatagcac tgggcggcca caacgggttc ccggctcttc ggtctgacga acacctgcca
    34081 gccacccccg tgacgtccta catacatcac cccctctgaa ccgtagatct cgatccgggt
    34141 ggcacagtgc tgccaaaggg ggaacatgtc gctgttgcgc acttctggat caatctttaa
    34201 catgtacggt gtgtacaacg tcagttcgaa cgtcacgact aatccatcaa actcgtaaac
    34261 ggcaatctgg gtatcggggg tctcggccgc gccctccgag tcaaagcgac cgccgaccga
    34321 gtacacggtc ttaggataat ccacgccaag tagccagcga gccaggtcaa tttgatggct
    34381 cgcgtcgttg gcgatatcgc ctccggaata cctccaaaaa tggtgccaat ggtactgata
    34441 gttcgggttg tacttggcct cgggggctgg gccattccag atgtcccaat tcaacccggc
    34501 cggaggatca ctatcgggac gcacggggaa attctcccag agcttttgat tgaaaatgcg
    34561 acacagatgg atgcgtccga gcttacctga ggagatgtat tctttcgctt gccagttgta
    34621 gggcgcactg cggttctgcg taccgatttg gacaacccgt tggtacttcc tggcagcctc
    34681 taccatctta cgaccttccc aagcattatg ggatgttggc ttctcgacat agacatcctt
    34741 gcccgcctga caagcccaca ctgtactaag ggcgtgccag tgatcggggg tcgccgagat
    34801 aatggcatcg accgccttgt cgtcgagggc ccgccgcata tcgtccgaca cctgtggccg
    34861 cgccccctgg agttgctcga cggtcttggc cagggcttcg gcacgcggca ggaagggatc
    34921 acaaacccag gcgatgcggc aatccccgcg ctccagaaaa ccggtggcta aggacgtccc
    34981 tcgaccacca cacccgacaa ttcccaagac gatctgttgg ctgggggacg actcgccaaa
    35041 gacctggaca ccggtcagca ctgtggcagc acccacacct aaagtgccgg ctgatttcag
    35101 aaaagaccgg cgatcggttg cgcggccgtt accgtggtta cgagattcgc tttggaacat
    35161 agctcgacct cctctagaaa acgcgtttat ttgtggcctc cccaacgact ggattatctc
    35221 aagaaccccg gctgagggaa actacttctt acccatggcc cgctgcgatt ggaaaaattg
    35281 cgttaagatg gtcccgcatt ccattgccag cacaccactg ataacaacac accgatggtt
    35341 caggcgaggg tcttcgagaa gccgatacaa agatcgcacg gcaccgccct tgggatcgtc
    35401 ggccccataa acaaccacgg gaatccgggc catgataatt gcccccgcac acatcgggca
    35461 aggttccaat gtgacgtaca gtgtacaatc ttccaatcgc caggactgga ttgcctccgc
    35521 cgcctgcgtg atcgcaatca tttcagcatg agccgtcggg tccttcagtt gttctctttg
    35581 gttatgggcg gcggcaataa ttcggccgtt tttaacaatt accgcaccga tagggacctc
    35641 attttcatgc ttggcttgtt ctgcctcccg gagcgcctgc cgcatgaaat ggacgtgtat
    35701 gtcttcatct cccattgagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35821 cacacgtttt cggacacaga attttaccca atagagttgt cacgtgacag agcttggcca
    35881 cccgaccgac tcccgatggg ccattgtcgg aatgaccacg gctctgaaaa aagtggaaca
    35941 gatagacttg gttgtgcttg ccacaaggac attgaggctt gaccggcata gatgtcattc
    36001 gaaagggaca cgggtatgag atttgcaatt tgcaatgaaa cctttaatga tcgctctgta
    36061 gaagcgggat tcgaattggc tgcagaatgc ggatacgagg gaatagagat tgcccctttt
    36121 acgttagccg cctatgtcac ggagttgaca ccccagcgcc gaacggaaat ccggcgttcg
    36181 atcgagaaat gcggcctgac gaccgtggga ctgcattggc tgctggcaaa aacagaaggg
    36241 tttcatttga ctagccctga tcccgctgtc cgggcgagaa ccctggagta tttgaaggcc
    36301 ttggctgaat gctgtgccga tttgggcggt cgcatcctgg ttttcgggtc gcctcaacag
    36361 cggaatgtcc tgccagagac aaaccgcctt caggcagagg cctttgcgat tgaggtcttg
    36421 gaaggactcc tccccacatt ggagcgaacc ggtactattg tggcactgga gccccttggc
    36481 ccgcaggaga ccaattttgt caatacggcc gaggaagccg tagcgattat taagaaactg
    36541 tcctcgccac acattcaact tcatctcgat tgcaaggcga tggccagcga ggccatccca
    36601 atacctgatc tgatccgtac caacaaacag tatttggtgc atttccacgc caacgacccc
    36661 aatcggcttg gcccgggatt tggcgaactg gattttgtac cgatctttaa ggccttgctc
    36721 gacgcgggct atcagggctg ggtctccgtt gaggtcttcg actaccaacc tggctgtgaa
    36781 cggatcgccc gggaaagtat tgcctatatg caacgatgtt tgaccgaggc tcaaaagcgc
    36841 taggtggcac gaggccaccc tggcgctgcg tcaaagacgt actgagcgca ttctgggacg
    36901 ggtcttcggc gaatgacatt tcgctatcgg ccgatcgtgt gtaggggtta aggcgattga
    36961 tgaggatctt cggtagactc cgtcggcgta atcagtttgc cggtgcgctt gtcccacacg
    37021 atggttcggt ggtacatgcg gtggcgccca tgacagttgg tacaggtgag ctttttggga
    37081 tcggtgatct ctcggcaacg ttctaggtaa acctcgacca cttcccgggc ggatgcgttg
    37141 tgatttttgt ggcagctttt acacgccgga tcgatctcgt ccggggcgta catcttgtcc
    37201 ggcggggtcg tattgttttc atcacttcgg tgcgcaagtg attccccgtg gcaatcgacg
    37261 caaccgatct tggctttgaa atgtcgggta ctcaacggtt catcctcaaa attggcgtga
    37321 cacacaaagc aaggtgaatt gtccggaggg gccttctctt gaacggtacc tttttcttcg
    37381 gcgaccgaca gacccgccca aacaaactga cttctcggcg ccgtgatggc gaaaacaacc
    37441 cataacgcaa cgagaaaacc cactaacgtc gcaaagagaa cataaccagt tgccaaacca
    37501 gatctgtcca ttttcctcat catctccgat cttcgatcac tcctagaact atttcgggcc
    37561 gttgatactt cactcatcag atataggaac tggttcgaag tcgtcaggat ttacccatcc
    37621 cgcccgcact tcctcgccac tcatgaaccg ttcataaaag ttttgggttc tcggatcgat
    37681 ataaggatat ttctcgaaga tgtcaccctg gccgaacatg cggggatcac cttcttgccg
    37741 aaggatctct gtcatctttt gtgacaggcg ttgggcaacc tccgggtact ctttgatgag
    37801 attgcggctg cattcgggat cttccacgac gttatatagt tcctcaggtg gacgctttcc
    37861 aaagcaagcc ttccaatagg cagtattgcg attttcccgg cgggcgtgca ggacctccgt
    37921 ttttgttggg cttccgtcac aatttaaata accggtttca ggattgccag caggccagcg
    37981 gctcggctcg taattccgca gataaagcca ttgaccttcc cgaatacctc ggatgggata
    38041 gccccagtca tgcggtcggc caacatcgtg gcgttctttt cccacgagga caaaacttcg
    38101 ggaaggatcc acctgcccaa cccggtcgct tattaagata tctaaaaagc tgcggcccgc
    38161 gaaagctgcc atgcccgatt gagccgcgtt aatcccagcc aactccaaaa aggtcgtggc
    38221 aaaatcggca aaactgataa agtcatcaac gacccgcccc ggatgtgaaa tccctttttt
    38281 ccacatgatc gccaacggca tatgattgga aagctcgtaa cactgtcctt tgacgcgtgg
    38341 aaaaggcatc ccgttgtctg acgtgacaat gaccaccgtg ttttccagtt cgcctgcttg
    38401 ttccaaaagt tccagcatct tttggaggtg ccgatcgaag tgttccacct caaaggcgta
    38461 atcgagaaga tcggtccgca ctatgtcgtt gtccggccag tacggcggga tccagttgac
    38521 atccgagagc ttcttacctg ttaagttcag cccactttgg aattggtagg cccgatgagg
    38581 ctcggttgac ccataccaga agaaccacgg ccgatcttgc ggtttctcct tcaggaactc
    38641 tgcaaagttc gccgcgtagt cattccgcga aatggtcggc gtcggaggtg tggtcgtatg
    38701 cttgtcaaaa gacttaccca aaagtggacg cggctgtcca tttgggtccc tagctcgccc
    38761 cggcccccac cccttaccgg tctttcctac aaagtaaccg tgttcctcta agacctcggt
    38821 aacgaccttg aactccagcg gaaactcgca attgtggtta caggcctcct tgagttgcca
    38881 ggaattgcga ccggtaagga tgatagaccg actcggcgca catttggcat tgggggtata
    38941 agcccgggtg aagaggattc cctcctttgc gacgcgatcg aatccagggg tttgaatcca
    39001 tgggcagcca tatgcactgg cgtgcggata tgaccagtca tcggccaagc agaaaaggat
    39061 attagggcgc tcttccgcca acgtcgtgac aaaggacgca gtgatcgacc cgaaaaacac
    39121 cgacaacaat actagatggg gatatcctgg aactcttttt ccgaacatgg gcgtgcctcc
    39181 ccgtcgaaaa ccgtggtttc accaaaagca gccgtcacag cgatattgta aaaagtgccg
    39241 gcgaatcctc aaggtatgct ttcatcgatc cgtgccctta tcaacagtgc tctttaggac
    39301 gtagcttccg ctcgtgtgaa acgcctttgg ccgcggacga aaatcgaaac cagatggggt
    39361 agctccaaag tgtacccgac tggatgagag gcccaatggc acagtagagt tggctatgga
    39421 ctcgccctgg tgtccgatgt gataccaacc ggccctctaa tcacttgaca caaccacgac
    39481 tgcgaatggc ccaaatcagg tcacatctaa cacagaaaac ctggagcagg gtgccccgtt
    39541 acatcccgtg tactcccgaa ctgcatatca accatttgac gcggctttga tggcgcgtga
    39601 cccttcgcag gcaacaattc cgtcgactat tccacgatat tgttctcatc gcagacacga
    39661 atggcagcgg gtacgacacg tgagaacaaa cggacggctg caataatctg aaacgccggt
    39721 atcccgccct cgtagggaag aaaatagtcc gcaatcagaa catcatcacg tttttcgggg
    39781 atcgaaaagc gggcaaaaat gaacttgtcg ttaagggcat tgctcagttc tagcttctcc
    39841 tcaagaggtg accattccag gaccccgtag attgccatgt acttgagaag gcgtctttct
    39901 tcatcgatcg tcaccaggac acgagggcca tccgtctcta cgacaaggtc gccatcgcta
    39961 tcgaatgtgg tcttgaagaa cgcccgtctg aagagcgccg ccaggttctc ctttgtcaca
    40021 tcactttcca tcaacagttc aacgtccgcc atactcctct cccttcgcaa gtttcacgta
    40081 tttttgcctg ccagaccctc agatcgtgag gaaccccgcc gtgaatcgag ctcccgaaat
    40141 cgtgccgata tccccgaagc tgcgcctgat ttagttatac tgacatattc tcgtaatgca
    40201 aatgattcgt gctgctctgg ggctagtgaa atgtggcgat tgactcccac caggatttcg
    40261 tttatccatg gatgccaaca gaagccggtt gcttgttcga aacaatcgca gtgagaggcc
    40321 atttgtgttg cagggagccg atgatgtccc agccgctgga aaagccggtg tcgtccagtc
    40381 catccggaaa acggcgttgg tatcaggcca ttggtccggc actgatcacc gcttgcgtgg
    40441 tatttgggcc gggcagtttg atcattagct ccaacgtggg ggcagtctat cgctttcagt
    40501 ttctctggtt gctcatcctg accgggctat taatggggac gtatgttgcc atggccgcac
    40561 ggatcggtgt ctgcggcggg gccagcccct gcacactctt ggcccgtcgc ttggggcgtc
    40621 ctgtcgcgtt ttttgtggga atggttcttt gcctggtctg cgccacattt cagttttcca
    40681 ataatcttgc cgtggccttg gccgctggcg cctttcttcc tgaaaggatc aatctcacga
    40741 ttgggggaac ggtccagcaa ttgccggtac ttcctacgct tttgatcatt ctcaatggta
    40801 tgatcatcct ctttctgttt gtcggtcgcc aaatctacaa aaccttggaa cgagggatga
    40861 aggtcatggt ggccatcatt cttttgtgtt ttgttttcaa tcttgtggtc gcccgccccg
    40921 atgtgattgg cgttttgcgt gggtttatcc ccagttggcc ggagggcctt cagcttggca
    40981 ttccgaaaaa gattggccaa acaattgacg atccgatgat tctcttagct tcactcttag
    41041 gaacgacgtt ttccgtggga ggtgcgtttt tccagggaaa tctcgtccgc gaaaagaact
    41101 ggtcgattca cgattaccag cacggaatca ttgactcgat tgcaggggta gcagtactga
    41161 cgggcgtcag catgatcatt atgatcaccg ccgccacagt catcccggga aaaccggctc
    41221 acgatgtggg tgagctggcc agatcgctcg agcccctatt gggacccacc tcttacactc
    41281 tgttttgcct ggggctgttc gccgtggcta tgaacccgat cgtgatcaac gccgtcatcg
    41341 ggggaaccat ttttgccgat ggtttaggac ttcctgcccg actgaatgac cgcgcccccc
    41401 gcatcctaac cgtccttgtt atgctcatcg ggatgggcgt cgctttgtgg gcgttgacaa
    41461 gtggtcagcg cccggtgccc ttaatcatct tcggtcaggc cctcaccgtc atagggaatc
    41521 ccttaatggc ggccgcttta ctctggctgg ctaatcagcc cgatatcatg ggggaaaagc
    41581 gcaaccgtct ggtatccaat ctccttggtg gattgggatt tctcgttgtg ttattggtcg
    41641 cggcacgtgt tctctggcgg gtgatcttac acttcgcgta acgggcaact tcatacgacc
    41701 aaacgtgtgg atcggctgcg taaaacgtac gttaccgagg atggtcagga gcaacgcaaa
    41761 cgtgaaactg cacgaccgca acattttctt taggaatcga gatcgagaag cgattggccc
    41821 tgagtgataa gtctactgca ctgagcattt aaatctcgag gaatctcttc acacgttcgg
    41881 aacagatcga ggagcagccg atggtacgtc gcccgatgta gtcgcgttag ttcatgatga
    41941 acgcggatgg acaccaaata cgcactaaaa cggtgacggt tttcaatgac tttctgcaac
    42001 ttttctcgta tcacccggcg gagctccgga gtgtatttac gcttcttgcc aaaccgtcgc
    42061 atcaaggtcc ccaactgctc ccagttaatg aatgtggcaa tccttatcgc ccgaaggatc
    42121 tcttgattgc cttccccgag cggatgagga gtaaggtcac ccatctcctg agacgctaaa
    42181 acttctttga tcaggcgact ctcgcgggcg gcacgctgga ttagcctagc aaaatgcatc
    42241 cgtttgaatg cgggaaaccc aacccatttg aggtccgacc aaaggtcata acctaaactt
    42301 ccatcgaggt actcaggatc aaatcgcgcg cggaggtctg tcgcaaatag gaacaacggc
    42361 accaggaaac gatcaagcat caagacgcct tccgtgggat tgttgaacac acagcgcaaa
    42421 attctagccc gttcgcgaag cagggcctga cgagcatcct caccaagggc cgctacccag
    42481 ggttcttcgg attcttccgg ccacaaaagg gcttgagcgt tgaaatagtc ggtgttgaac
    42541 tgccgattga gcctaatcac ggcttcgccc actcgcaagg caatggaccg cgtgatcaag
    42601 cgaaaccaaa tccttccgaa aaggcgtaag ggtaaatcac gcacggagtc ccaaaacccg
    42661 taatgaacga gggaccgcac aatcataatt gggaccgccg cggcttgttg ctcgtcgagc
    42721 ttcacgtcat tcggatcaaa gtacccgcca ttgtaccaat cctccatgaa gcggaggtca
    42781 atgcccaggt ggtggcgctg tcgtgattcc ggctgagggc gtgctaaccc aaatcgggcc
    42841 cgtcgcaaaa acttgattct tcctctctcc acaacgtcgg acggaaggcc ctcgtcggaa
    42901 cacagctcag agggacactc tgcctcatga ggtcgcaccc cagtgagatg tgtcccgagt
    42961 ttccattcat actggtaaac ccgttcccaa agcgattcca ggtgcatttc ctggttccac
    43021 ttgcgataat tctccataag gtccaaatcg gcagcctgcg caacattcaa aagccgcgat
    43081 ctgaacgcga gaggaattaa tttgaggaga agcccggtcg tcagcataaa actgcctact
    43141 aaaagaggca tcaaaattgc gacacgtgcg tcgtaggttt tcacgccggg gacgagatta
    43201 cctaggtatc caacaaaaga gacgaacgcg gtggtaaacg tgtatcgaag gcttcgtcgg
    43261 taaaggaagt tccctgcaag gtaaagggca tcgaaaaaac tgaagagccg atacgccgta
    43321 cgcaaccagt tcatcacgcc cctcaattgc tgtagttaag gtggcccgac aattcagtgt
    43381 tggtgggaac tttgttcgat tgcgcccctt gattttaatc aaaaaaggtt aggtctgcga
    43441 acctgagcct tcgttgaagc gagcgtttgt caacccttat gtcgctcgct gcttagaaaa
    43501 acgcattgtc cggtcctcgc gctctgttca agtgactcaa tgattctttg cgtttgatga
    43561 aggtcttcaa tatcactcat gcgctgcacg agactggtga ccatccgatg aaaacaaaat
    43621 agcagtcgtt ccccaagcgg acgttcatta tcgaatgact cctggtgacg tccggcctcg
    43681 tcaaaccaaa cgagagtggt tgggagatcc acaaaggcaa ccccccgttc gcaaaccacc
    43741 tgaagctcgg ccaagggacg ataattgacg gcctcacgcc aatggggcgg caaatactta
    43801 ccacagctaa tttgggcaag aacatttggc tcgcgcccct ctgaggccga accgaaattc
    43861 agactcatca tcatgtaatc aacgtcgtca ggatgcgacg aacgatgaac cacgcccatc
    43921 accttgatag gagaccgtcc ggcaacaaac cggcaccaat ccacaagttc gatgaggtcc
    43981 aagtgcaatg cttgacttgg cgtgatatgg gacaagccat tgcacttaag atagtgtgac
    44041 agtcggcgat gacagaacaa cagccgcggt gaacccaaga cagtggccat caattcttta
    44101 agacggacag tggcggggga ataccgtcgg gcaaactcca ccataaaagc cacaccggat
    44161 tggcggaccg catctcgaat ctgggtcgtt tcctccagcg acaaaacgac ccgcgaacct
    44221 aggtacaccg cctttccata ttcgcaggcc gccaaaatgg gcaagggacc tagccaatgg
    44281 gtgttcaaaa ccaacacggc gtcaatgtct ggtcgacgac ataaggtccg aaaactgtcg
    44341 tgcgtggtaa tgccgacgtc gcgcgcagcc agggctgctc tttgggccac cggctcatag
    44401 attgcggtca aaacaaaacg gtcctctagg accctcaagg ccgggagata ccgtttttcc
    44461 cactgatcat tcagtccaat gacgcctaaa cggagcttca tgactttccc tgtcttgcaa
    44521 cgccaacaca accatcggcc actgtgacct gcgtaagaga ctcaacgatc ggtcgcttcc
    44581 ggtgcccaca aagattttga caatcggaca tgccccaaca ggccctgctg ggcaggctcg
    44641 ttggcgaaca tcggttgaat gccggtccat ttcaaatcgg ccacaaggga cccgtcggga
    44701 tttcggccca caagttgcga aacaaatagg tctcgcaaat gatatcggtt ttcagcgtcg
    44761 agaccggcca cctccgtcac atgagtgatt ttgcgagaac catcctcggt aaaacgggca
    44821 atctggacca cgagatggat ggccgaggcg acctgtgccc gggcaacata aaccgggata
    44881 tccacgtcgg acatgagcga taacgtctcc aggcgaatta gggcatcccg aggggaactc
    44941 gcgtgaattg tagaaaggct tccggaatgc ccactgagca tcgactggat catatcgagg
    45001 gcttccccgc cgcggacttc tcccacgatg atccgatcag gacgcatccg cagagaattg
    45061 ctaaacaact gacggatcgt cattccttgg ccttctcggc ctccgtgacg ggcttccaaa
    45121 tagatcgtgt ggggctgaac gaagcgaagc tcggatgtat cctcgatgac gacaattcgt
    45181 tcctcaaaag ggatggcgcg ggagagggca cccaggagga ccgtctttcc ggtccctgtt
    45241 ccaccggaaa tcagaatatt cttgcgaatc cggacacaaa tctccaagaa ttccttgact
    45301 acttccgaga gtgagttcag gcggacgagg tcttcgagcg tcatcgcctc cttggtaaac
    45361 tttcgaatcg tgacgcaggt gccgatccgc gatgtcggcg gaaggatggc gtgcacccgt
    45421 gacccgtcag gcaagcgggc atcgaggaca gggtgctcgg ggccaatctc ccggccaacc
    45481 cattgggcaa cattgttaat ggcagaaatg agggcatcct cgcttgggaa tcgggcgtct
    45541 gtcttttcga gcttaccccg acgctcgatg taaacttcga aggggccatt gatcatgatc
    45601 tcgctgacag agtcatcctc caaatagggg aggatgggtg acaagaaata ccgggtgcta
    45661 gcctcgaaga ttgattggcg ggacatgtct tctaccctgt agccaccgaa aactgcctaa
    45721 tctagtagcg aaagaataca atctttggcg atgtgttcgt tcagtttaga caaatttaag
    45781 ccctttttgc cacgtatttc ccaaagatgg ggaagtcacc acgacgccag gtttacacga
    45841 cgccaggttt atgggtgaca tcctatcgcc accatctacc atcggtcgca caaagtccgt
    45901 gcggaactaa caattaacct cgacgacgtt gccctgtaac gaatggcttc tggaagggcc
    45961 cattggtggc gagccagacg cgaaccaccc ttccgagcgg taaccggacc gcttggccaa
    46021 gagtccactt tctgttatgc taatcgttaa ggaaacgaat ggaaatggta gagcgatccg
    46081 gcaaaccgga ggtttcttgt tgccgccagc gtagctcaat cggcagagca ccgcattcgt
    46141 aatgcgggtg ttgggggttc gagtcccccc gttggctctg gcgacggctg attcgtgacg
    46201 agtagggcag cgtttttcgg gtggatcacg cgcgggaaca acactcacac gccggtcggc
    46261 gggcgttaat gttcccagta atagtttgtc ggggtcaaga gaccggcggg tgaggcgttt
    46321 gcaattggcc cgtgtgcatt tctttacatc gtttcgatga gggagtcgat tgaatggctg
    46381 aggcaacact cggcgcagat gtccggcaga tcgtgttgag taatgcggcg ttcggtccgc
    46441 gcgatgtgaa ggcgctcgcc gatgcgatgg ccgaagattc cagtcagtac cgcgctctgc
    46501 gggacgccgt ggcggaactc gagttgcggg aagaaacgcc ggccagcaat gtccgtttgg
    46561 ggtgctgttt gtatctgctc gggcattttg agcgagcaat cgatgtcctc aagcgaggag
    46621 ataacggtgc attggctcac tactatcttg gtcgggcata tatcgcgacc cagaagttcg
    46681 acctggcttt agaaagtttt cgagcggccc gcatagcggg ttacaattcc gacgagtgcg
    46741 ccctcggtga agtcgaggcc ctccgcctaa aaggcaacgc tgcggaggcc ttacaaatct
    46801 tggatcgcct ttcgggaccg gtcgtagaaa gtgctgagta ccttttccaa cggggtgcca
    46861 ccctggcatg cctgggagcg agtcgagacg aagtaatcga gctctacgaa cgggccgtgc
    46921 agagtgatcc gtatcatgtg ggtgccctgt tcggtttggg tcttgaaaat gatcgacagg
    46981 gcaacgacga ggtagcgctc gaattgtacc agcggtgcgt gtcgcgtttt ccgcctcacc
    47041 taggagcgct cataaatctt ggcatccttt atgaggatgc tgaacaatac gaaaaggctg
    47101 tgcgatgcta tcagcgggtt ttggatgctt ttccggatca tccacgggct cggttatttt
    47161 tgaaggatgc ccaggccgcg ggacgcatcg tgttagacga ggatgtggct cgccgaaagg
    47221 accggctcgc ccaaattttg gcgacacccg tgacagattt cgagttgtct gttcgaagtc
    47281 gaaattgcct gcaaaagatg ggcattatga cattgggcga cctgtgtcgc tgcacagaac
    47341 aggatttgct cgccagtaag aatttcggtg agaccagcct tgctgagatc aaggagatgc
    47401 tggcctcaaa gggactgcgt ctcggtcaac tccttcccga acgtgcggct gtagaacccc
    47461 tcggagaacc tgaagagccg ctcgctcccg aagaagaggc cttgctggcc aagcccgtct
    47521 cggagttaaa tctttcggtg cgggcgagaa aatgcatggc gcgccttggg atcaataccg
    47581 ttggtgagct gatccgcaaa agtggcgacg aacttttgga atgcaaaaac tttggcgtca
    47641 caagcctcaa tgaagttcgc gaaaagttgc gggctttagg gttgaaattg cgtggtgatt
    47701 gagacccgaa cggtggtgtg gggtaggcga caattccaca aactccggcg aaggccagcg
    47761 gagcctttat tgctggtgat ttgtctggac ctgggctgtt gatgtactcc tttgatgcac
    47821 tcggcgcaag agtcgtttca ggttatctgc gaagacccac actgtgctgc ccggtgcggc
    47881 atcctaagaa cggcccatgg tgaggtgcag actccggcct tcatgcccgt gggaacgcgg
    47941 ggttcgatca agggtgtgtt acccgagttg gtctgtcgta ccgggacgca gatcatcctt
    48001 tcgaacacct accatttact gttgaggccc ggtcctgaga tcgttgccga gttaggcggt
    48061 ttgcatcgtt ttatggggtg ggacgggcct attcttacag acagcggcgg atttcaggtc
    48121 ttcagtctgg cacagcttgc aaagatcgac gaaactgggg ccacctttcg ctcccatatt
    48181 gatggacagc gtgtggtctt aacgccggaa ggctccatct cggttcagga gatgctaggc
    48241 tctgacgtgg ccatggtttt tgaccacgtg gtggggctgc ctaacgagcg gaccgttatc
    48301 aaagaagcca tggaacggag tcttcgctgg gctgagcgct gcaaggttgc ccactccaga
    48361 aaggaacagt tactttttgc cattgttcaa ggtggtctcg agccggattt gaggatccga
    48421 tgcgctgaag ccctaattaa cctggggttt gacggttatg ccgttggcgg tttgagtgta
    48481 ggggagccac cacaggagat gtatcgcatt cttgacgtga cggtccccgt gctgccaaga
    48541 acacgccccc ggtatcttat gggcgttggg cgtcccgttg atttgattga ggccatttat
    48601 cgcgggatcg acatgttcga ttgcgtgatc ccaacgcgga atggacgcaa cgctatggca
    48661 ttcaccgatg atgggccaat gcgtatgagg aatgctcgct atgagcgaga ccctcgcccc
    48721 atcgacccca cgtgtccttg tcctgcctgt tctttcagcc gcggctacat tcgacatttg
    48781 tttatggtca aagaaatgct cggccctatc cttcttactc tgcataacct gacgtactac
    48841 cagcgtctga tccagcgggc ccgaagtgcc atctgcgagg gccgtttttg cgaattcagg
    48901 caggaaatgc tatccaaact cgctgcggga aacgacgcca atgatacata aatggctccg
    48961 cacggcggga aaaaattggt taccagacag gaaagaaagc tatccactac ggcgttcgtt
    49021 cgatcggggc gaacgcggga ccgaagtcag gcgcggcaaa agcaagcttc gcgtgaggtg
    49081 acaactgtcg cttccttgag ggccgctccc gcaagtcttc gtaggtggct aacatctccc
    49141 taatctcttc ctgcatccgc tgttgtaact cctcaggttc aaggaccttg gcatatttcc
    49201 caaaactaag aatccaccgt tggatttcct ccaaactgtc cagatcaaac tcggcaatga
    49261 cggaaccatc acgctgggga tatagccgct ggctggcatg ccatgtcgct tcgcttacat
    49321 agcgcgcaac gacgggagaa aacaaaaccc gcacatgata atcgccatcg ccctggaaga
    49381 taccgaaact gccggcaaga tgtttttcaa gggaaaatcc ttctgggaag tcaaacggct
    49441 gatcggttat tttgacagcc tcaacgcgat tgaccttcca atggcgaatt tcgttgcatt
    49501 caaccaagag tcccacaaga taaagtgccc ctcggtgata ggtcaaccca tagggatgca
    49561 ccgtgtacgt ggtggcttcc ggcgccttca acgaatggta tcgcaattca atagccttcc
    49621 ggtcttcaat ggcaagcata atctggtcga tgagctcagc cttcttggcg taatcgcaca
    49681 tccccgtccg ggttctataa aaagaatggg ccatcttttc cacgtaggcc caagccgtct
    49741 cattgaaaac actgcggatt ttgttgaagg cactttgcgc agccgtccag aacatcgtcc
    49801 ccgcgagagg ttccatcaga caccgtgcaa gatgcaaggc aatcgcctca tcgtaagtga
    49861 taggtaatcc tttttctatg agatcaccgg ttagcctcca acgtttcttg ccatgttcac
    49921 caacagtctc atctaaggga aaacccgctt gtatgaacat gtcgagatca cgacgaatcg
    49981 ttttctcgct cacttccagg gtctgggcca tttcgcgaac cgtgacaccg aaccgccgtg
    50041 acgccagcat gcggagaaga atccattgac gcaccaaggg tgagttttcg gacatggcac
    50101 atccttcgtc aattgggtta acccggtaga cgcgatgagt tggcatctgc tcaaccgacc
    50161 accaggatcg atccgaattt ccctttacgt tcgatcatat actcactgaa gcttgaagtg
    50221 aattccgccg cttgatgaag aagcgattca ggatatgcac acgagaagct actgacagcg
    50281 tgtagtcatc ctatggaaaa gtgggatcgc caaatgccag aaatgggcct ctttgccccg
    50341 gttgaccgtc gaaagccccc gacgacgcaa tcctactctc gtcaatccgc tcagcaaacc
    50401 cgcattcaga acattcaggc tactcttgct ctgcgatatg aaactgccag agtcaggaac
    50461 taaacgtcat cttcatcctc ttcgacatgg tgttcttcgt cgagcgagcg gtcttcttcg
    50521 attggggcct gtgtcactgt ggtttttcgt tcctcgggcg gcaggacggc gatgggtcgc
    50581 gcaccaaaca gctcggcagt tttcctcaca aatggctcct gaaggatatc aaaaagtaac
    50641 cgttcgcctg tccccgggct tgatttctcc ggcgcacctt cgtttgccag aactagttct
    50701 agctcaattt tattgccggt gatcgtctgg agagcccttt caaatcgggt cttgatttct
    50761 ggacgcgaac aataatccct acagaacgcc ttatctttgc ggaacgtcac gacgacccgg
    50821 ttaccgccgg cgactgccac ctgggagaat tgcttcgcct gatcggctgc cagtcccccc
    50881 acagactccg ctgctttaac ccagacattg agaacatttt cactcgtgag tgcctcgccc
    50941 gatgggtaag ccggtaatgt cccttcgcgt ggcactgaag ccttgactgc ctctttttgc
    51001 ctttccaatt cagccttggc ttgctcaacg gcctgcctcg cttgtgcggc accctgttga
    51061 tcgccagccg gaggttgtgg cgaaccagga cttggtctcc ccaagactcc tgccaactca
    51121 ctggcgagcg tttttggtgc ttgatgcaac ggttgtgaga taccaatccc ctcgacagaa
    51181 cgggcctgat ccggcgttgc gacagtcggc aagctcttgc cggaccgtat ggcttcaatc
    51241 agtttgccta cgtactccaa ttctcccagc cgggacaatc ggacgatggc caactcggcg
    51301 agaatccggt cgtgggtagc aaaccgcatc cgtgccaagg tgtgatcaag aagctgcatt
    51361 gacgccagca gtcgtggtag ccccaatcgc tgtcccaact ccgaggctac tgaaagttcc
    51421 ccaggcccca cagaaagaag catgtctcct gagcaaccca atgtcgcaac catgcagtca
    51481 cgcaggtaag tgaggagctg ctcaattaaa agccccgcat ccgcgccgtc cttaagggct
    51541 tgatgaaata ccgccagagc ctggctggca tcttcctttg tggctgcctc caaaatggcc
    51601 ttgaagactt cagcccgggc catccctagg agctcaagca cgtccgatac ggcaatattc
    51661 ttcccaccgc taaaagaaag caactgctcg aggagcgact gggcatcgcg catcgaaccg
    51721 gcggcacgtc gcgcgaggag ctccaagacg cccggctcta cggtggcccc ctcggcttcg
    51781 acaatctgct gcaatcgcgc tacaatggtc tgtgtttcga ttcccgcgaa atcgaatcgc
    51841 tggcaccgcg aaaggattgt gacgggaact ttgttcggct ccgtcgtgca gaagataaac
    51901 ttcacatgtt caggaggttc ttcgagcgtc ttgaggaggg cgttaaaagc ctccttcgtc
    51961 agcatgtgga cttcgtcgat gatatagatt ttgtatcgcc cgcgactcgg tcgaatcacg
    52021 gcgttttgcc gcaattgtcg gatctcttcg atgccgcggt tgcttgcccc atcgatttca
    52081 ataacgtcaa tgtcatcccc cgagccgatg ctccgacaga tatcgcacat attacacggc
    52141 tcaggtgttg ggcccttaac gcaattgagg gccttggcca gaatgcgggc ggtcgaagtc
    52201 tttccaacac cacgggcccc cgtaaaaaga taagcgtgtc cgacccggcc actaacaata
    52261 gcgtttctca acgccgtggc gacgtggtcc tgtccgacaa gttcctcaaa agtctggggc
    52321 cgataacgcc gagcaacaac gaaatatcct gtcgaagtgc tgcctgactt cgcctgactc
    52381 gttaccgtct cttcgttagg cttgaccttc ttggaacgag cggccatcca aacacatccc
    52441 tctgtatctt cccatctcgg acatgggccg ccccgcctgc ttcaccgcat acttcaagga
    52501 aagccggagc acaatgatcc gatgcccatc ttgataagtg cgtactcccg agttatcgcc
    52561 ctgacccgga gcgagaggcc cactacacat ggggatgact gcttatggct gctgactttc
    52621 gtccctgacc aggttcgcag ctccccattg tagggacccc tcgctccggg ccatagccct
    52681 gctatcattc ttatgaatgg tgatgggcga agtcaaaggg gggtctcatc tcacagtgac
    52741 agaaagatcc gcggcttccg gccgcagaac ccgccaagaa tcggcggtaa cctatattgt
    52801 taatgggaac tgacaacggc tgcgcccttt taccgtgaga ttgatgccaa ggaactagat
    52861 ttgaggagca tgtggcatgc ctgttgaaaa agccgtcgtc cttgcccggg gattgggaac
    52921 ccggatgagg aaagaggatg cgagcgtcat cctcagcgaa gagcaggaga agattgcccg
    52981 caagggtatc aaagcactaa tccctgtagc tgcgggccga ccatttttgg actatgtcct
    53041 ctctgccctg gccgatgcgg gaatcaccag ggtgtgcctc gtcgttgccc cggaccacga
    53101 tgaactggtc gcgcgatacg gaggcgaagt ccccttggag cgactgtcgg tgagctttgc
    53161 gatccaggag aaaccattag ggacggccaa cgccgtggcc gccgctgagg agtttgtaaa
    53221 tggcgatccg ttcctcatgg tcaattccga caattactac cctcacgatg ccctagccgc
    53281 actgcgcgaa ctgtcgggcc cggcggttgc cctctttgaa caggaggcta tgttgaccca
    53341 gagcaatatc gcggcggaaa ggattttgag tttcgccgtg ggactcgtcg atgcaaacgg
    53401 ctatcttgtt cgaatcctcg aaaaaccatc acgggagacg cttgctcact tgccgcgtcc
    53461 aatttggctt tccatgaatt gctggcgatt cggaccaagc atctttgagg cctgccgccg
    53521 catcggccct tcgccccgtg gggaatatga aattaccgac gccgtgcaat atgcaatcga
    53581 ccacctccaa gagcgtttcc aaaccgtctg cgtgaaaaag gcggtgtatg acatgaccag
    53641 ccgtgccgac attccggcca tcgtggcaag acttgaaggg atcaaggtct ggctctgacc
    53701 tgcgggtagt caatgatgac gcgaccccgc ttacatttcg cccgcgaact ttcgcaccgc
    53761 ggactcgagg gcttcccaac gatccccaaa ggcctcaacg aagtcggcca ctcgtcgttg
    53821 cggcgatagg gtctcgaacg gtgccaggcg attgatcgtt gtcaggtagc gcacgaagcc
    53881 ttcatggtgt tttttgtgaa gataggtcac caggatccac gatagcccgt aggcggtttc
    53941 ggggctactc gagaaaaggc tgtccgaaag cacgagccgt cgtaaccaac cgggggattg
    54001 ttttgccaaa acatgcctgg cctgaagctt taggtcttgg ggttgagaga tggctcgacc
    54061 ggacacctta ctgatggggt tgacgaagtc gccttcaaac atcagcgcca tcccctcatg
    54121 gaaccaaagg ggtacatcgg caaatcggtt aaccagtccc atgtttgtcg ctacttgatg
    54181 aaatgcttcg tggcgagtaa cattgggaag tttcgatcca ctagtgggcg aattgcccga
    54241 ccgttgctga ggggcatcca ggacgaaggt tacaacgcga ttggaaacgg ggtcatagca
    54301 tccaagcgtg ttggatagcg cgtccgagtc tttcttgaga cgggctaagt aatccaggta
    54361 ctctgcccgg gaggctaaca caatggctat gagggggaag gagctctctt gtagtgggag
    54421 cttcctttgg gcggcaaacc gttgtacgtt gcggtaggtc gcttcaaaaa cctcgcggca
    54481 atcctccgtc atgctggcag gtcgcacgac aatgtagtgg cttgtcgtgt ccaccgtata
    54541 acttcggcca aattcgcgga ggagttgggc ccgaatcgct ccgggatcga gcgccacgaa
    54601 gtcatcatta accacgtgaa aatccctcgc cagccgacgt tccgcccgaa cgagggcacc
    54661 gtcacgtcct aggacaaaaa tttcatccga cgtccaataa aggagttgtc cctcatagcg
    54721 atcactgccc acgcggaagg aaatcattcc gctcacgaga ctcggcggaa ccgattggga
    54781 atagcatcga aaggacgtga gcccaaaaaa gacagtcaaa atgagagata taattggttc
    54841 gtggcggacc attgggtgag tgacattccg agatgaattc ctgccgagtg ccgcgactaa
    54901 cggaattcct ggcgagtcga atgttttcca aaacgatttt ttccacattg tttgacccca
    54961 aatcgcgtgg ggcggcatac aagtggcctt tgtagtggga tttgaacgga tttagttcac
    55021 gcggctataa aaaagtctag ctcaattcat cacgggtgcg cggagcacgc tcctcagcct
    55081 tggaaaaggc cgcagagccg gtataagaag aaaacccgac catggcgata tacctggatc
    55141 acaacgccac aagcccgatt cgttccgaag ttgccgaggc cgtccgcgag gcctggttta
    55201 cataccacgg gaacccggca agtcagcacg cctccggaag ggccgcccgg gcacggttgg
    55261 ccagtgcccg ggaaaaaatt cttgcccttc ttgatgccga tacatctcac aacggagatc
    55321 ggatcatctt taccagtggc gggaccgagg ccaataacct cgccatcctt gggatggcta
    55381 tggcgcgaac aggcggcgcc ccgggacgga ttatcgtctc cgcaatcgag catcctagcg
    55441 tcctgcgggc tgccgaacac ctcctcgacc tcgggtggcg gctcgacaca ctcgatgtct
    55501 cccgagatgg cctgatcaac ctcgattctt tgcgagcctg gattggcccc gatgtggccc
    55561 tggttagtgt aacgttggca aatcacgaga caggtgtaat ccagccagtt gctgaggttg
    55621 cagcaatttg ccatttccac ggtgtcccag ttcacacgga cgctgtccag gcgattggca
    55681 agatacccgt ctctttccga ctcctcggcg ttgacgcgat gacggtgacc gcccacaagt
    55741 gcggtggccc gctcggcatc ggtgcgctca ttgtccgccg tgaccaaccg ttgctgccca
    55801 tcctttttgg tggggaacag caagacggac ttcggcccgg cactgaatct gtggccctgg
    55861 ccgtggggat ggaggtaacc ttcgaactgg cactccatga gctcgatgac acgatgcctc
    55921 gaatgaggtc ccttcaggcc cagtttgagg actatttgaa gcgagagatc gctgacatag
    55981 tggtccacgg ccaaggagcg ccacggttac cgcagactac ctccctcgca gtccgcggta
    56041 tcgataacca actcctcctg gcagctctag atgccgaggg gatcgaatgt tccatcggct
    56101 cagcttgttc cagcggctct gccgaaccct ccgccacgct ccttgccatg ggtgttcctc
    56161 gcgacctggt gcgatcgtcc ctacgattca gcttcgggcc gcgaactacc caagaggaat
    56221 tgataaccgc ggcaagcacc ttggccgact tggtgaggcg tcttcgcggc aagcgccaaa
    56281 ccccacgatg atactttgat actttagcta ccacctcgtg ccccgtgctc aattcgctgc
    56341 tcgaatacta atgcggctaa gcggatacca gccgtcggcc gttgtcccct caatagccag
    56401 tcgaagaaca ggtacgggcc ggctcgcgct ttcgaccacc ccgattgcca actcatactc
    56461 gcctagcggg accgtagcag gtaccgtcaa agtgtccttt acgttcatta cttccccggg
    56521 aggcaactct ggcggttgtg caaggaaagc tggggtgaac acatccacag aaccaggtag
    56581 gaactggttt accgtcacat tgtcgctaac gatgacgaag ctcttgcctg tcttgtggcg
    56641 aagccggtaa gccaccggat aagctcgata acacggagcc gatcccacat tttgccactc
    56701 catggtgatg ggaagctgtt gcccacggcc gatctcacgc ggatgagaca gcgaccgcaa
    56761 cacgaggcga taacccaatc gacggagaaa tcgttcgacc tcggccttaa cttcatcgcc
    56821 cggcgggagg ggggctgatt tgttattgat gtacgaggca tgaagggcca gggcgtaatt
    56881 gaaaatgtac cgcaacgacc acccttcgtc gacccatttt cgaatatccc aacatgtttc
    56941 ccaagccacc ggcgctcgct tccatacatc gagcgcattc gccttggtca gtgtctgtgg
    57001 gtatgcgttt cgcatgtgac accaattggc agaaaaacca cccatgtcgc caaggcaatc
    57061 cgcccgccat cccgtccctc gctcggtcgc gtatcgcagc atttcaggcc catcgatgag
    57121 catgagaagg ggagttttct taaacgaggc cagataggcg tcaataatgg ctttttgatt
    57181 ttccggggtg ggcatttttg ctgggtccga gcgactcatg tgccattctc cccaccatcc
    57241 cacagagccc aaatccacgt gatccaagtc ggggtgaccg tcataccgtg cccccaaccg
    57301 gcggatgaag tcaagatggg cccgcaggac cacagggtcg tcgagatcgg gaacctccag
    57361 cccctcggtg tcgccatatc ttgtggtgat gacacgtcca ccgatggcct ttagccaatc
    57421 gggatggtat ggtcgcttag gcgttgtcga gcaacacatg acacgaaacg ccagtcgctg
    57481 gcccgcacga cgtgactcgg ccagttgttg atcgagaaac tcgtaatcaa tttcgcccgg
    57541 acgcggttcg agtcgccccc acccccaacg agcgtactga acggttgagg gaatccattc
    57601 cggcaagttt tgatctcgat cggcggtacg atggaacgtc tcccaaccca tatgtggatt
    57661 gggaaggata gcatcgatct ccttcggttt gaccgtcgtc cattcttcgg cagaaacagt
    57721 ccgcactcca tgaaatgcca gtgctaggca aacaccgaaa acaatagtga cgtgcagtcg
    57781 cattgaagac cctttcacca attttgattg cctaccatct ttctgaaaaa cccagaccat
    57841 tcaccctagc caaacaagct ccgacgttcc tttgggcaaa tcgaaacaaa gtgtcattaa
    57901 ccgctcggta ccgttcgttc cgcttccttc gcaatatgtg ccagacgtca agtcaagcag
    57961 acggccaact cgtcgcaacc gttgctgttc ctcgcccgcc cgaccaactg ccgggacacc
    58021 ctccgccaga ggtgtcaact ggatcgtaaa caaaggtgcc gaaaacgggg cagaaaagac
    58081 gaccttcgta tcgttcttct cgattcttgt cagctcccgg gccgcccaat agcgggcgat
    58141 ttccgctaga ttcatccaga tcaggttttc gtaccgagca tgtagtcgtt ggacgaccgt
    58201 cttcagaatc tcaaaaccac gttcctctcc gttaaagtaa atccctggcc agtggcaaac
    58261 catgatggcc ggttcttcac gaccgatcac atccaccatc cgtcccttcg ctaaatctgg
    58321 tgtgatgaaa agatcggcgg acccctccgt ggtcccatcc cagccaccaa accagtcacc
    58381 cgtgcagcct ataatcgaca caacacactt aggatcatct ccatcaagcc cggaggcata
    58441 ttcgacccgg ggtgccacac ttctctcgtc ggtgaaaagg tcacgaaagt aatgcggaat
    58501 ctccgtgcga aacacgtccc gacacgcctc cagtgtggcc tgggcaagtt gcggacgggc
    58561 acggctacca aacccaccgg gtgtcgttat cccttcgcaa tgcagatcga cattctttaa
    58621 gattcgcaac gcatatgcca ggtagtccgt tagctcatct acggaacgcc cctcgctcca
    58681 tccccaattc tccataaatc gggctgaccg ctcctcatat ggccgtcctg tcttcgtatt
    58741 gataacccag gtatgggtaa ccatctcggg atgaatatcc caccggggca taatccgttc
    58801 ccggactagc cgaaggcttt caacaagctc ctggtgcgac cagcccggca ggtcacgatc
    58861 tagccaaccg acacaagccg gataaggaac aacgctgtac tttcctttga ccccgtgctc
    58921 ctcgcaccag tccataaatc gccgcacgaa actatcgggg atttcacgag gaaactcctt
    58981 ccagggtttc tgatattccg cccggcctgg ccacacctcg gcaaactgtg gcatcgcaaa
    59041 gtgggccaga ttcaccaagc aagtggagtc atcaatgatt aacgaaaccg gtacccgatt
    59101 tcggggattg agaacccgaa tggcactgtc cctgaccggt ctttcgcccg ggacctggag
    59161 ggaggcggga agcgctcgat tgctgtttag gattcccgcc gccagcgagg caccggcaag
    59221 ctgaacgaaa cggcgtcgcg tgagttgttt catggcgtgg acctcctcaa cttttcatcc
    59281 aagaacacta gccgatggtc cccggtaccg caacgaccct tgccaggggc gccgtagtcc
    59341 tgaaatcgcc atagtcgtcg ttcgtccgcg cactctacca atagcccatc ttaggcagta
    59401 aactaagtat ttttgctgca tcgggctacg ctcaatctcg ggcgtctttt ggattcattg
    59461 attgtcagag agccaaccta gcggcaccag agggcataca cggcaaacct caaagtggag
    59521 gataaccgat gaattacgaa gaatcacctc ccgaatttga cgatgaaccg gtcgacgacc
    59581 tggactctga ccaggaggac ggggagttgc caccgccccc gccggtttcg ccggaggatg
    59641 actttgtcga attcgatgag gacgacttcg acgatgactt tgacgatgat ttcgagtttg
    59701 agccagagta cgaggccgaa atcgagcgcg agttcggcga tatcgaccaa ggcgaggaga
    59761 tacctgaaga aactgaggct gaagaagaac cggaagaacc agaaaaagtc ctccaagagc
    59821 cgggcgagtt tccggagggc gacttaacgg atgacgagtt cacagaatca gacgaggagg
    59881 agttctaggg agaatctacc ggactaaaga ctggaaagca gtttgatttt ggcttgacag
    59941 atctcaagat gaggtgaacc aacgtatcgg ctatgagtga aacacttgtt gaaggaattc
    60001 aggccgccgt gcaatttatc cggcagaagt gggataagga ggcatacgcc gggatcattc
    60061 tggggacggg tctcgggacg ttggctcaag agatcaaagc cgacgccgtc atccgctatg
    60121 aggacattcc gcatttccct cgctcgacga ccataagtca tacgggccaa tttgtatgcg
    60181 gtaccctgga gggcttaccc atcgtggcca tggaagggcg attccacgtc tatgagggtt
    60241 acacgttcca acaggtgaca ttccccgttc gcgtcatgaa ggcattgggg gccaatcttt
    60301 tgatcgtttc caatgcctgc ggcgggatga acccccatta ccgccttggc gacatcgtcg
    60361 tcatcgacga ccatatcaat ttgatgaacg ggaacccgct catcggtcca aatgacgact
    60421 cattggggcc acgatttccc gatatgtcgc aaccttacga tcaaacatta atcgacgttg
    60481 ccctggcgat cgctcggcgg gaggattttg tcgcgcaccg cggggtgtat gtcgccgtga
    60541 cgggccccaa cctcgagacc cgtgctgagt accgcttcct gcgggggatc ggtgccgatg
    60601 tcgtggggat gtccaccgtc cccgaagtca ttgtcgccgt ccatgccgga atgagggtct
    60661 tggggttgtc cgtggtcacc gatatgtgcc tccccgatgc ccttaagcca gccgttgtgg
    60721 aggagatcct taaggttgca gccgaggcgg aaccaaagtt gcgcaagatt gtgctggggg
    60781 tccttcgctt tgaggcagaa aaacagcagc gccgaccggg ctaaccgtag gcagtgtcca
    60841 tgactcgaaa taagcctggt agcgagtcca ccagacctta aagctgctta acggcgaagt
    60901 acacctccgg accccttaaa ggtgggtgcg aaagaactaa ctgaagcggt tcattcgaaa
    60961 gagaagtctt atgtttcggc ctgttgatcg gaatccatcc tttcccaaac tcgaagaagg
    61021 catcatccgt ttttggaagg aaaaccggat ttacgagcgt tcgctcgact ggcgacgggg
    61081 cgcgccctcg tttgtgttct acgaaggacc acccacggcg aatggcctcc cccatcccgg
    61141 gcattgtttg acgcgggcga tcaaagacct ctttccgcgt tacaagacga tgcgggggta
    61201 ttattgcgaa cgccgcgccg gctgggacac ccacggccta ccagtggaag tcgaggtctg
    61261 taaagagttg ggcattcact ctaaaaaaga catcgaggag ttcggtgtcg aaccgttcat
    61321 tcgccgctgt attgacagtg tgttccgcta cacccgtgag tgggaacaac tcaccgagcg
    61381 ccttggtttc tggattcagc ttgacaacgc ttacgtgacg tatcatcaaa gttatgttga
    61441 aagcgtctgg tgggcactta agcaactctt cgaaaagggg ctgctctatc agggctacaa
    61501 gattgtctgg tggtgggccc aagggggaac ggcactttca gcggctgaag tcggccaagg
    61561 ctaccgctcg gttgcggacc ctagtgtcta tgtcaggttc ccacttcggg atgagccgga
    61621 cgccgacctt ctggtttgga caaccactcc ttggacgttg cccagcaatc aatttgccgc
    61681 cgtccgaccg gatttggact actgtcatgt cctttttgaa ggcgaaaaac gagccgtcgt
    61741 tattgcccag gccctcgttg agacggtgg
//
LOCUS       DAOSUT010000002        60522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_341_length_60522_cov_9.823871, whole genome shotgun sequence.
ACCESSION   DAOSUT010000002 DAOSUT010000000
VERSION     DAOSUT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 60522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_60522_cov_9.823871"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1447..1520)
                     /locus_tag="PLD05_00265"
     tRNA            complement(1447..1520)
                     /locus_tag="PLD05_00265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Val,seq:gac)
     gene            1974..2900
                     /gene="speB"
                     /locus_tag="PLD05_00270"
     CDS             1974..2900
                     /gene="speB"
                     /locus_tag="PLD05_00270"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOP75887.1"
                     /translation="MIPPRSHFQAGYNFLGLPDELADPQRAQVWILPVPYEATTTYGA
                     GTRNGPTAIIMASRQVELYDYRRGCEPASELGFATLPPLEIHSGSPTAMISRIEEVVL
                     HIFGGDPQPRFLVALGGEHSISAGIASAAARIFDPHEIVAVQIDAHADLRDEFEGSPY
                     NHACAARRILNCLPLMQIGIRNISEEEDRFRSTAHRLITIFADEWTEAAHERLAEFVR
                     NKLVYLTIDLDGLDPSIMPAVGTPEPGGLSWQQIQEVIRIIAQNARGVPIMDVVELAP
                     IPGLIAPDFLAAKLIYWAASDFLLREERKAAL"
     gene            complement(3608..5722)
                     /locus_tag="PLD05_00275"
     CDS             complement(3608..5722)
                     /locus_tag="PLD05_00275"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOP75888.1"
                     /translation="MSSMTRLIASTTVLLFISLISGRQTGVWANDGPAGRPPAVPLVV
                     HDPYFSIWSFTDQLAADWPRHWTGRIHALCSLIRIDGRPYRLMGMAPADIPPLEQTSL
                     EVTPTRTIYLWRTQEVEVRLTFLTPVLPHDMALLSRPVTYLIWDVRSLDGKPRIVDLY
                     FDATGELAVNEPNQLVTWHRGQTEHLDWMRIGTTEQPILGKKGDNLRIDWGYAYVAAV
                     RHPQTNTVVADHETARNHFCNTGRIPRRDDGNKPRAVQDRWPVLVCMHSAKVSRDESL
                     KWHILLAYDDIVSIEYLGQRLRPYWRQRYADISEALAAAEEELPDLMARCQAFDEELL
                     ADARSLGGVGYANLIAISYRHTLGAHKLTLGPHGEPMYFSKECFSNGCIGTVDVIYPA
                     APIFMLFNNELLKATVVPVLDYAASPRWKFPFAPHDLGTYPLANGQVYGGGETSEEHQ
                     MPVEESANLLILAYAISRLDGNTAFADRYTKLWEQWAHYLEEKGFDPENQLCTDDFTG
                     PLAHNCNLSIKATIALACYAEMCHMSGKIEEANKYRKLAEEFAQKWIAAADDGDHYRL
                     AFDKPGTWSQKYNLVWDKLFGLNIFPSVVRQKEIAFYKKQLQPYGLPLDNRALFTKTD
                     WTVWTATLADSREDFDALMNPLYRFIDHTPDRVPLTDWYWTHDAKLRGFRARPVIGGV
                     FIKFLESPDLWKKWATRCARMK"
     gene            6003..6194
                     /locus_tag="PLD05_00280"
     CDS             6003..6194
                     /locus_tag="PLD05_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75889.1"
                     /translation="MHRTIPPVSGYWRLTSCQGIDSRSQGAARLPWAAITLRAINLEQ
                     FSTKTPVGLRLSRKGDSYE"
     gene            6187..7848
                     /locus_tag="PLD05_00285"
     CDS             6187..7848
                     /locus_tag="PLD05_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOP75890.1"
                     /translation="MNKTVTSVLCAVILWGGLGFAADPISLYESPTPAQPNGELDRIV
                     FAKLQTLGVQPILCSDAVFIRRAFLDMTGTIPTAQEVREFLDDQTPDKRARLVDRLME
                     RKEFLDYWSMKWGDALRIKAEIPVNLWPNGARAFHRYIWASLASNKPYDQFVREMLTA
                     SGSNFRDGAVNFYRAVQDRSPTGIASTVALTLMGVRTDHWPKERLEGMAVFFSQIAYK
                     PTSEWKEQVVFWDPWGLRLVPTNFVPGREAPGTAPRKSEDPIVTPTPADISQEPLVGV
                     FPDGTKVTIPADRDPREVFADWLIRPENPWFTRCIVNRIWVWIMGRGIIHEPDDIRDD
                     NPPSNPELLSYLEKELIQSGYNLRHLYKVILTSSTYQLTSIPRKPSPEVEANFGYYVP
                     RRLDAEILIDALNKITGATDLYTSAIPEPYTYIPRDLTAVSLADGSVISPFLTLFGRS
                     ARSTGAFNERSNKPTPSQWLYMLNSSQVYNKIATSQKLGELFRSGRPVPELIEEVYLT
                     VLSRRPSEEEMTIAREYIEKSRSRGEAAMDVVWALINTEEFLYRH"
     gene            7872..9239
                     /locus_tag="PLD05_00290"
     CDS             7872..9239
                     /locus_tag="PLD05_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOP75891.1"
                     /translation="MNKNNHYNEPKVSRREILQQGIYGAGGVLLGKSLLSQVRAQEAA
                     PAPSTPPKIEPKAKSVIQIFLWGGMSHNDTWDPKPKSGRDYLGEFTEVIDTNVPGIQL
                     SSLFPKLARQADKFSLIRSMTHGNNGHETAAYIMQTGHLPGERLAYPSIGAVFAYFKG
                     KEYKGIIPPYVVLTRSPGRFSEEGFLGPNFKSFATGGDPNATRFEVEGVVARGITDDR
                     QKARRDLLEKLNTFGRLVVDHPEIALAEENKKAAYELILGPGRDVFDLSKEDPKLRDA
                     YGRHTFGQSCLVARRLVEAGVPYIVINYPGGWDTHSNHFTTMRRQCPQLDEGLAMLLQ
                     DLHDRGLLETTLVWCTGEFGRTPKVDWQPPWNGGRNHYGKVFTVLVAGGGFKGGRVVG
                     ASNELAEEVAERPVYPVDLLGSIYALAGIDYTAKLPHPEGLEARVLPEPGDRVKSNGL
                     LTEII"
     gene            9381..11501
                     /locus_tag="PLD05_00295"
     CDS             9381..11501
                     /locus_tag="PLD05_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOP75892.1"
                     /translation="MKRYSVLVIIVAMASLAFGTAAFGQAYIGFVYPAGAQQGTTTQI
                     RLGGQRIDIPVGAIVTGTGVEAKLVEYRWYLDNQQVAVMREQLQQLQRVAKEKEKNNE
                     PLDDATRQLMERIQDRINDWQNFPANRAIVNLAIVEVTVAPDAEPGPREIRLVTTQGV
                     TNPLPFYVGQFPEYVRKPIKACQFPVLGNEQAAERRRPPEEKEVRVTIPCVVNGQVAP
                     GFVNAYRFEARKGQRLLIKAYTRDLIPYMADAVPGWFQSMLTLYDAQGQEVAFSDGFR
                     FHPDPVIYYEVPQDGEYVLTISDALFRGREDFVYRISIAESPFITSVFPLGRKLGESI
                     TPEVKGWNMEGAELRLPPADAGSGVYFVTAVKNGLVSNRWPFAVDTLPEIMEKEPNNK
                     PAEAEKVSLPVIINGRIDQPGDLDVFCVEGKAGQTLVAEVTARRLDSPLDSVLRITDA
                     SGKPIALNDDYEDPGMGQMTYHADSYIMIPLPADGAYYVYLGDTTRKGGPEYAYRLRL
                     SEPRPDFALRVVPSAVAVRSASANTVRVYAIRKDGFAGDIQVSLKDPPEGFANAPVVL
                     RANQEMVGLTIKTSLKDSDGAIPLRVEGRAKVGDQEIVHEAVAAEDRMQAFLWRHLLP
                     VADLRVRVYDPNYQPQPARTPPEVPPEMVEAARKAAQEAGRAPFSKAQVAGRVRMLNL
                     LYQNGFLTDDFYLVKMAECAAAQD"
     gene            complement(11740..12630)
                     /locus_tag="PLD05_00300"
     CDS             complement(11740..12630)
                     /locus_tag="PLD05_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOP75893.1"
                     /translation="MRNHLSHVLVPVLLGLGLLALSGVPVVAEAPPVRVMSFNIRYGT
                     APDGENHWDRRKEFLVETILAFQPDLLGTQETLAFQRDYLAEKLPGYGVLGVGREDGK
                     DQGEIMAIYWRKDRFDVLESGHFWLSTTPDVPGSKSWDSALPRMVTWVKLRDRKAPNL
                     RPILWFNTHFDHRGEQARQESARLLRKKLLTMGEDCSLIVTGDFNAAEGSRPYQLLFG
                     DTEGQPSPIVDSFRAAHPERGEEEGTFSGFRAENTKGARIDWIGCSRDWKVLEAGIDR
                     TARQGRTPSDHFPVFAILTR"
     gene            13021..13857
                     /locus_tag="PLD05_00305"
     CDS             13021..13857
                     /locus_tag="PLD05_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75894.1"
                     /translation="MTRCLLLINEPGSGPWNMAMDEFCATHAEREDVPILRLYRWREP
                     TLSLGYFQRYQDYQRHRLNGLCPVVRRPSGGGAILHDLEWTYSLAFPTSRVTDRDRLS
                     FYRAVHQTILTWLDSLGLKGEIIQEQPPPCKSNNTDCAFLCFQRRAVGDVVVPLSPEM
                     HTLVSAGATGEPVPTDVKIVGSAQRRYKNTVLQHGSILLGRSVLTPELPGVMEILASK
                     GIQLTHDEISYHFVSHWPQTVARALGWRLQPWTLSLTDSPKFAELEAKYLSRAWTCKS
                     QA"
     gene            complement(13898..16474)
                     /locus_tag="PLD05_00310"
     CDS             complement(13898..16474)
                     /locus_tag="PLD05_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75895.1"
                     /translation="MRLVVHALAFLSLLGCARDVLAEASGIFPKAVTRKQSLTLGFGE
                     GTQGFEPVSQCRIAVENGRLVVESTGHDPYIVKAMNLPGEAYEVSLRLRTTVRGGGAI
                     YWTSQKSLTWGEDKSRHFFLIADGEWHSYTVRIDTPGGLRGLRIDPGTAPGIYEIESI
                     QLTELQTCPINIEFIERTDEGVQFHIQNASNAPVTVRMEQHSLVIPAGQAIDIIRKFD
                     GQRPVELVTLDLQLPDFTTDLRHSVVVYSDSAPAEWMAIATGGDTAVLVELAAKDNVL
                     RVRRGETVLAALAPIVAPADPNGAGLIATIPPLQATPIPNGVQVQGKGVQGTIVVDKD
                     ELILNLEATDENSEIEGPVVRAFGLLEQGVFAGLEYLGKGEKSSSTLDIETEEHIRYA
                     PDRLNVTMPLMAFVTDRASVGLTWNDMGLQPIYATPNVFDGTQEHRMSLRGHHIEARL
                     RIAQGMVEDMILWATKRWGLPPLPEPPRSESEQFQLCLWALTDGPIRNEQGWGHCVEP
                     NWARRFHVDMVSTIWRLSGTLPDVPHLVPGGGHLSDDSAWFLTGRAAEWLAHMRNQAR
                     GLMAQQQPDGSFRYSGKYLKGHYEDTASGYCARPAAVLLEYAKLTGDKQALEAGLKTL
                     DYMKRFRVPRGAQTWELSLHTPDILASAHLVSAYVRGYELTKRQDYLDEARRWALSGI
                     PFVYLWAEHPIMLYATIPVYGATNWRAPNWIGLPVQWCGLVYAYALTELAPYDNTLDW
                     RHLAEGILIAGEQMQVPREEGDYAGLLPDSFHLRYQRRQGPFINPCALALLRSAIKGR
                     PHRLAVAIGDNHRVVAPFPVKIEGNEAVIQARAGVSYQIVIDGEQIKTIASQGEDRIS
                     LE"
     gene            16854..17210
                     /locus_tag="PLD05_00315"
     CDS             16854..17210
                     /locus_tag="PLD05_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75896.1"
                     /translation="MKRVLSRVWREDEGVLTFEWILLITVLVIGVVGGLSAVRDAVIT
                     ELGDVVEAMISLDQSYYIAHPWDVQVPDCITDGASDSSYTDSAGMAQGRLKPWDIAQD
                     QGAFEGCSDAVENPRR"
     gene            17489..17812
                     /locus_tag="PLD05_00320"
     CDS             17489..17812
                     /locus_tag="PLD05_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75897.1"
                     /translation="MKAVFQTIFRSLHDDHGVLTFEWILLLTLVVIGIVGGLSAARDA
                     LISELGDVVGAVVHIDQSYTVSSDATGWGNEFSYSDELPECAGEQNRPTTGRPDQGPV
                     GGCSQ"
     gene            17997..18230
                     /locus_tag="PLD05_00325"
     CDS             17997..18230
                     /locus_tag="PLD05_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75898.1"
                     /translation="MTFEWLILITLLVIGVIGAYSAVRDGMVDELGDTATAILSVDQS
                     FHTEPPERLPCAGSWGSWEDDDAAEQLERGRNL"
     gene            18365..18913
                     /locus_tag="PLD05_00330"
     CDS             18365..18913
                     /locus_tag="PLD05_00330"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOP75899.1"
                     /translation="MFVAIKAFPILAIAVGVIAGWAAFTDLRQRRIPNFVTVPAALLG
                     LLFHSLLPSGEGWWFSLAGFAVGAVLLVLPYLLGGGGMGDIKLLAALGSWLGPFWILV
                     AFATGTVCGAVLTLICLVTGRSLEELLGISQGAHDDVKKPTQPRAIKALPFRSKRALP
                     FAVPLAMGTWIMLAVVLIRAGL"
     gene            19322..20539
                     /gene="cpaB"
                     /locus_tag="PLD05_00335"
     CDS             19322..20539
                     /gene="cpaB"
                     /locus_tag="PLD05_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOP75900.1"
                     /translation="MKRISPAGVSFFVGAILVGLVAAYAVRHLSQTSPETVTVNPRKT
                     KTIVVAKYNLPQYARITEDYIDEVQVPADKVPEGALTLKSRALYRLAKTTILAGQPIR
                     DDDLYAVGEVPLLSERLPPGYRAVTLAVDAKAAVNGMIQPESLVDITLTVKNDRPEIG
                     GLATLTLLRRVRVLATSRNRFPASEDRPGDLRNITVAVTPEQANKLILAEKYGTLSVV
                     LCSKLEEETDEATLEDIMASKGEVPVIPTSNPVTRADSTLSEPSNDLVNIYSLLGISP
                     LTPEPMPEQPVTKTAQIWRGGNVEEVTFVEWQIREAENATLVAEGKTPQPFTPGTLTS
                     SPKTTKTHDAEAEDCPECAAKRAKEAAARSVGEMPVPTPASQLVPRNDQANHLPGGYG
                     RVVTVPVRGQLAN"
     gene            20658..22037
                     /locus_tag="PLD05_00340"
     CDS             20658..22037
                     /locus_tag="PLD05_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOP75901.1"
                     /translation="MMSFLYRQSDKDGNGRKNVLTEAASPETWESEFQKLKTGLHKEI
                     IESLDLSRLAKMDDDQLRRHVRNLTEAVLRRRSDLLGNIDEERLINELMAESFGLGPL
                     EPYMQAPDVTDILVNGPFEVYVERCGRLQATNTVFADEEHLLQIIQRVVSRVGRRIDE
                     QNPMVDARLPDGSRVNAVIPPLALNGPVLSIRRFGHERLKIADLIAFGSICQEIVELL
                     RAAVEGRINILISGGAGSGKTTLLNNLSAFIPEDERLVTIEDSAELKLQRKHVVSLET
                     RISNAEQRGEVTPRDLVRNALRMRPDRIILGEVRGAEALDMLQAMNTGHEGSLTTIHA
                     NDARDALSRLEVMVSMAGFELPVNVVRQYIGSAITLVVHLARLKGGVRRVMRVAEIIG
                     CSNGDYQMQELFGFQQEGVDGAGRAKGHFYATGNVPRFLKRLEEQGIKLPESLFQKRV
                     LTPAITSLE"
     gene            22051..22983
                     /locus_tag="PLD05_00345"
     CDS             22051..22983
                     /locus_tag="PLD05_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP75902.1"
                     /translation="MAWAVWLYPILTFTAVSGLLILFALMILGRRSKDSRNAEELRPE
                     DLDLEEILYIPPAKAARTALDRWFYELLEAAGSRLDRSTASLLPAAGAVIGAAIPVVF
                     FDSVFWAIVGTVIGAFAPIGFWKFQAFRRVRRMRKQLPVVLQLFADAVRSGLSLERAT
                     ALAAQESKGPLQAEFAWCATQLELGHSPVRVMERMAQRIPIPEFRIFATAALVHRRTG
                     GDLANLADRLARSAQDRTEFYGHLKAVTAGSRLSVFGLTAGVFIAVVLLGLTRPEYIQ
                     QFLNHPSGLSILGIAAGLQVVGLLWVWRILKARY"
     gene            23049..24128
                     /locus_tag="PLD05_00350"
     CDS             23049..24128
                     /locus_tag="PLD05_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP75903.1"
                     /translation="MPDILLLALGYWQDLAHLWSEWGLSILTFANVVLLAMIIRWILV
                     RRAQPKASAGLPPVVDPETDDPGAFGALTPALAAQIPESAKETRDFQLLLRQAGLHRP
                     SARYTIYAFRFLLLVTPLIIAGVCAVIAPQAWTLPILVAGGLSSAVLSIIPRMYVFLR
                     RRKRYEEIRSGLADTIDMLGMCLTGGLSLLDSLEHVAGQMGAYPALAEELRTVRRQAE
                     VGSLKLALEDLTRRVDLPEVRQLCAVLSRGTQLGSQLASSLSEHADHLRVALKQAALA
                     RANKAPVKLVLPILFCLAPAALIVLTAPAFLELKQFFDPSLMKSASGKGVSFGTNSVV
                     DAIRDLDQRVIFEPPPTEIVPPISQ"
     gene            24434..25774
                     /locus_tag="PLD05_00355"
     CDS             24434..25774
                     /locus_tag="PLD05_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOP75904.1"
                     /translation="MSQTPMMRPLASTPRTGIESRQLSFDELKQRIHSKLVDKLDLSK
                     IGQLEGEVLRREIRLVVEHLCDSENTMLNRSERERLIDEVLDETFGLGPLEALLKDPT
                     ISDILINGPKNVYVERRGKLEKTNVCFRDNEHLLQIIDRIISKVGRRVDETCPMVDAR
                     MPDGSRFNAIIPPLALDGAAVSIRRFGRSPLKLEDLLNLKAFTPEMVMLLEGAIKARL
                     NIIIAGGTGSGKTTLLNTLSSFIPNNERIITIEDAAELQLQQEHVVRLETRPPNIEGK
                     GAITATDLVRNALRMRPERIIIGECRGPEALDMLQAMNTGHEGSMTTLHANSPRDALA
                     RLETMIMMAGFELPLKAMRTQIASAVNLLIQANRLQGGVRRITHITEVVGLEQDTIIT
                     QDIFRFEQDGVDENGRAYGRFVATGIRPTFMPRLEARGIKLPPSIFRQRTMMEA"
     gene            25845..26816
                     /locus_tag="PLD05_00360"
     CDS             25845..26816
                     /locus_tag="PLD05_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP75905.1"
                     /translation="MTQMTILIIVAAFVGAAALVVALSQLLNQRHGNRLESRLEALTH
                     QVKRDSESKERTPTVLAEPIEDKTSLTTQLLKYFPNLERLLEEADVGLAPGRLVAIAT
                     VLVGVGLAIGVVLKLHPALIAILPPALGSIPFLWILWRRRCRLKAFAAQLPEALDMLA
                     RALRSGQSLASGFNLVAKEIPAPVGKEFGRVFEEQNLGIPLEESLRDMAERIPNLDLK
                     FFATAVILQRQTGGDLAEILDRISSLIRERFTIWGQVQALTGEGRLSGIILMALPVVL
                     FLAIYRLNPDYVMTLFTDPLGQKMLAVAIVMQILGAIVIRKIVTIKV"
     gene            26853..27851
                     /locus_tag="PLD05_00365"
     CDS             26853..27851
                     /locus_tag="PLD05_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP75906.1"
                     /translation="MNQDVIFELVVAQGLDAAVFLPYAVFGMFAAIAFLVLERFSGTT
                     QRTVQRLEEFTRPQRRRLTVEELAERRPNRFAEVLEKTTPTFAKPLEPKNEVELGRLK
                     QRLAEAGFRGDKAVQIFLGLKFLSLLAGFVLGGSITLGLYGLSRNTTTSAIIAAGLGF
                     YLPDLVVTFLGRRRKEAIFLSLPDALDLLVVCVEAGLGLDQAMRKVTEELASSHPVIA
                     KEFALANLQLQMGRPRAEVLHEMGVRTGVQDVRALAAVIIQAEKFGSSIAQALRAQSH
                     AMRTRRRQLAEERAQKTAVKLIFPLVLFIFPGIFVVLVGPAAITMIRELFPKMAGG"
     gene            28027..29919
                     /locus_tag="PLD05_00370"
     CDS             28027..29919
                     /locus_tag="PLD05_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOP75907.1"
                     /translation="MMEKAEEIFEGKWYAHPRMRNALIAGLLTGAAFSLAHLGIIPDF
                     AEIAIYAVAILLGGYHWSREGIEEFVTEGEIGIEVLMIAATVGSAVLGMWDEAAFLVF
                     LYGTAEGLEEYTYARTRFSIRKLLDLAPKEARVLRDGEEITIPAEELKIGDILVVKPG
                     ESIATDGIIIEGRSSINEAPVTGESIPVEKKEGMKVFAATVNQEGALKLKVTATFKDN
                     TLSKMIHLVEESQEQKGKAQLFIEKFGKRYSPLVFLSALLLIVVPSLLGAPVSYWATR
                     AVVFLVAAAPCALVMSTPVAIAAGITRAGRSGVLIKGGICLENLGKIKAVAVDKTGTL
                     TKGEPVVTDVVPLNGEESEVLSLAYNVERYSEHPLAKAIIRKAEESRIRSQEVADFSA
                     IAGYGAKARVGNRVVYVGKEGLFRKLGLESQTIPQIETLRSHGKTVMLVGTETGIDAI
                     VAIRDRIRSEAKEAIDELHRMGIKVIMLTGDNEATARAIAEELGIDEVRADLKPEGKV
                     AAVKMLEEKYGTVAMVGDGINDAPALAQATVGIAMGTAGTDAAIEAADVALMADDLTK
                     VSFAINLGKRARRISLQNIVFSLLILAILIPAALSGVMSVAFAVFVHEASELLAVANG
                     LRMARG"
     gene            30142..31809
                     /locus_tag="PLD05_00375"
     CDS             30142..31809
                     /locus_tag="PLD05_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP75908.1"
                     /translation="MVSRRVVIVGGVAGGASCAARLRRLDENAEIFVFERGGFVSFAN
                     CGLPYYIGGVIAPRERLIVASPERFRDYFNIEIRVHHEVTKIDRENRIVHVRNCQTGT
                     SFTRPYDNLVLSPGARPIVPNVPGVDLPGVFTLRLLEDAERIYRWLEDRQVTRAVVVG
                     AGYIGLEMVENLVHRNIAVTLVEAANQILPGFDGEMVVPFYDKLAAHEVEILLGSPLC
                     AIEPHNSQLAVVAGGKRIPCGAVILCVGVRPEVTLAENAGLEIGELGGIRVDEQMRTT
                     DPSIFAVGDAVEVKDLVTGKWTWVPLAGVASRQARVAADAICGRLSQFRGALGTAIVT
                     AFGVTIAKTGLTEKAAQRDGVRYQKSYSHSPHHATYYPGAKSIAIKLLFDPQDGRILG
                     AQAVGETGVDKRIDIIAMAIQKGGTIFDLEEAELCYAPQFGSARDPINIAGFVAGNVC
                     RGDADLAYWEDLDHKPQADAPLVVDVRTPGEHAAGAPPRAINIPLGELRSRLHELPTD
                     RELWVHCGIGQRAYYAARILKQKGFRVKNLSGGYVTYRMYRRSKPAD"
     gene            32248..33462
                     /gene="ribB"
                     /locus_tag="PLD05_00380"
     CDS             32248..33462
                     /gene="ribB"
                     /locus_tag="PLD05_00380"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOP75909.1"
                     /translation="MSGVFSTIEAAIEAILEGKVIIVVDDENRENEGDFICAAEKITP
                     EIVNFMITYGRGILCAAILPDVAERLDLPLIVKENTSPLGTAFTVPVDHRSTRTGITA
                     MERATTIRALIDPTSKPGDFCRPGHVYPLIAKEGGVLRRAGHTEAAVDLARLAGLAPA
                     GVLCEILNDKGDRATRSELYELAKRFNLEMITVEELIRYRRRREKLVYRIAEAQLPTR
                     YGAGRIIAYGVKFENQEPIAVVIGDPTRVPAPLVRLHSSCFTGDLIASLRCDCGDQLH
                     LAMEMIGREGAGVLVYLPQEGRGIGLVEKIKAYHLQDQGMDTVEANLALGHQADCRDY
                     GIGIQILKDLGLSKVRLLTNNPKKTDAFIYGGFDLEVVDQIPIIGPINEYNARYLATK
                     RDKMGHLLPDTF"
     gene            33842..34696
                     /locus_tag="PLD05_00385"
     CDS             33842..34696
                     /locus_tag="PLD05_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOP75910.1"
                     /translation="MSAKVFRYAVWGLVTLVVAFGGLSVAGSAEEGKKIYILTDLEGA
                     SGVYKFAQTRESGPLNDQAKEYLMGDIAAVVRGLREGGATEIIVLDGHGSQAFVPHLM
                     VPGAKYITGIPRPSVMPYLDESFAGIVQLGAHAMMGTQDGVLCHTQSSRNENRYWYNG
                     VESGEIAQVALYAGAFGVPTIMVTGDEAACREARQFFGPEVVTVVVKVGLARESAMLF
                     PFEETRQALYEGAKRAMAQIGKCKPYVIDMPIKAKKQWLVFETPDGPGKLQTKEGVIE
                     DVRDIFRF"
     gene            complement(34728..34961)
                     /locus_tag="PLD05_00390"
     CDS             complement(34728..34961)
                     /locus_tag="PLD05_00390"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOP75911.1"
                     /translation="MSEANSSYVARIGATAGLVWHVLNENGPLNLSQIIKKVDRPRDE
                     VMQAIGWLAREDKLIFSEQGRARYVALKEKAVA"
     gene            35464..36534
                     /locus_tag="PLD05_00395"
     CDS             35464..36534
                     /locus_tag="PLD05_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOP75912.1"
                     /translation="MKSFWQIVLVGSLLTFGDLLPGKHDVFAQEVATTPEVPWTQVWT
                     QPSVVAEIETIVKAVRPEEIRRHLFYLAKDPLPFRKLNYTRPGQEKNTLYEADDYLTR
                     QLMSWGYEVRREEVRVQAFRRDPNKPKAHQYSPPASEDPWYLAYNLYAEKKGRRVPEE
                     IILLLAHKDSQSWVDSPGANDNAIGTSGLLEIARVLAQYPSDRTIRLLWCNEEHTPWT
                     SVTAAQKAKERGDKIIAVINLDGIGAKSAEDTAAGKKTNVILFTLPEGEPIAQLAFAA
                     NERFGIGLEQRIDKRPRPGDDDGSFVRAGYPAAVVMIGSYPYADPNYHTEDDIPERTD
                     VVNAAMTVQVALATVITLDRMP"
     gene            complement(36668..37036)
                     /locus_tag="PLD05_00400"
     CDS             complement(36668..37036)
                     /locus_tag="PLD05_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75913.1"
                     /translation="MINRSASLLVWSPRVLGIFVCLFLSVFALDAFGEGKTFLEGFRD
                     FLLHVSPMLVLPGIVAISWRWEWVGGAVFTGLAVLYAYWAYFVKGHPGWIPIVGGPLL
                     VVGVLFFLSWAHRCRSPHIQ"
     gene            complement(37511..38569)
                     /locus_tag="PLD05_00405"
     CDS             complement(37511..38569)
                     /locus_tag="PLD05_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75914.1"
                     /translation="MTAKHLHMASLWKFIVLLCLAAVALAIGCRTPQIVTQLERENYA
                     LESKIWELVSQLEQKQAELDACRSELERLKSRAGATSQKANPSSHPRELYAPTWPEGG
                     DMLAPAPSVGSSTEPFALPEVSVTPPSRETSPERALPGSTSHRPPEPSPEEKPSPEEN
                     SVTRPLLLAPSNSDAEPTPDILPQETTRRASPLDSSRVSSPASAIPISSPSSRGEKAD
                     QVGPMTERIILPPKFVTGRDYDGEDGDDGIAVLVQPRDSRGEVILEPAAISVVVVDPA
                     MTGPAARVARWDLQPEEVRSYAVRRGDVLGFLLELPWPNRLPEHEDLKVFVRYYAPDG
                     RYLEAQSDVRVSLARSGS"
     gene            39211..39284
                     /locus_tag="PLD05_00410"
     tRNA            39211..39284
                     /locus_tag="PLD05_00410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39245..39247,aa:Arg,seq:acg)
     gene            39864..41006
                     /locus_tag="PLD05_00415"
     CDS             39864..41006
                     /locus_tag="PLD05_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75915.1"
                     /translation="MVRCTALALSLPQQLSLRLLAIWSILLCASMGMVSPHILAGTFP
                     NLFTLNRVEADPNKDYWLSEENGPWMIMACSFTGDQAEQQARELVLELRKRYKLPAYM
                     YEKTFDLKAEGGSNPRRYRYQQGERIREVAVLVGDFPSVDDPRAQETLQKIKCYRPAA
                     LESRPEKSTSLTLAALRSIQQSLLTDDNEKKQKGPMRHAFLTTNPLLPDEYFAPKGVD
                     KFILALNEGYEYNLLNCPGRYSVQVAHFTGEVIIDQREIQAIERGEKTFRSSLVDAAR
                     KAHELTLALRQKGYEAYEFHDRGASIVTVGSFDSLGTRRPDGKIELDPRIVQIINTFA
                     AEPAPPVPGVPTLGYKPKTLIGIPFDIQPTPVEVPKRSFATDYVRR"
     gene            complement(41038..44301)
                     /locus_tag="PLD05_00420"
     CDS             complement(41038..44301)
                     /locus_tag="PLD05_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOP75916.1"
                     /translation="MRPSTQRTATRWGFNGRLIQALCAKVLPDMWYEVVLGAVVLTAL
                     CLGGCHRTAYRRQADMEVSTLLQQGQRDPRWRLRDTTVYPKKESRYYDPFCPDAEPMP
                     PDDPVSHQLMHYVDGKKGWPHWHDNGDTYYVESPFWRAYLPRDERGVVVLDRARAVDL
                     ALVHSREYQRQIENLYLSALNVTAERFRFDVQFFGGHSTFFTSNGSKRAGGPRSLLEL
                     DTDAQARKLGATGTELLVGFANSLVWQFSGSDNYASASLLNFSLVQPLLRAGGRAVIL
                     ESLTQAERNLLANIRQMAHYQRGFYLQVVTGTNPGAGPSAGGLSIGSLTPSVTPGRGG
                     VLGLLQTQVQIRNQEQNVQGLEDSYEQLLAAHQADRIDRYQVDLALQSLYNAQSQLLS
                     ARSSYQSQLDNFKVSLGLPPQLPVEISDDFLTQFDFVDPRLLNLQRRTTLLVRALRDP
                     ATPNPPDWLDQMRSIYEQTARQVEIVENDIARLTAVLPKRKTLLREFYSKLVTEDGQL
                     EGLTIADKAIADLDARYQAIIADFGLLLEGKLPAARGEERVPRQPLKQLLENLQQFIQ
                     DPDSVTNKRVSELEAQLAELQNQAKVADEELNKLSQTMATAPQEEVLALQAKYSEVRA
                     QSEILRSRIDSITRTLTELKASPKEQVAMQLLEPLLLQLSDLSLIQARARLDSVTLTP
                     VDITPEEALEIARENRLDWMNARAALVDQWRQIQIAANRLRSDLDVTFSGDLGTTTDN
                     PFSFDGNTGQLRIGFRFDAPFTRLVERNQYRSALIAFQRARRDYIAFEDQIAQSLRET
                     LRNIRLGQNTFELRRSAVLVSIAQVDLARERLRAPPRPGETSQFGVNTARDLVTALSG
                     LLSAQNAFLGAWVEYEVQRMVLDFQMGTMQLDDRGHWIDPGPIRSQNRGPGAEAVPAP
                     APQTEVRPLPTITANPTDSLNQGRFASGEEFKNRDSDENGTAISPVLPSGLTGASQGV
                     PVGESRNSGSQPHLSPALQRLKDRLASYQKSPDGFEERVFPPAASGGSVTVGQVGVGN
                     KASPAVADFRKGEPDFGTIQTTFAPTENGLPESLLPSIQSLLDLENEGVAVISDRPE"
     gene            44741..45883
                     /locus_tag="PLD05_00425"
     CDS             44741..45883
                     /locus_tag="PLD05_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75917.1"
                     /translation="MSIWNKILICLILLASLAMWYLGMRALATHRAWRTAAKRLQSEL
                     AQLEAERERLIHGDGTTKGIRQLQVELAKYTSQRGRVWNGCRPVRVDVRQDPQAGLVV
                     QVSVIVDRLGLAGLQEVQELPVDSLVYLFDERDIQQGGVYLGAFRVAAVTPRQQDQPA
                     MVQLESVLHLTDRELRRIQSAMQAAQQGGTGWTICELLPKDDHEIFAGLNPEALAQIL
                     PKKVVDEYIRDGKPSDPNNPNSPPFFRKLRDYESAIRYLHLKRSELMDQVAARTADKG
                     RLEAAVADSQREAKARDQEVAQLQEVLKRAEFERDLAVNHLKAVEDTLARVTVARDAL
                     IQQNRVTAAEIAQAQEKARQLIEQRVRSVAQSEQPASSVTAEGTRR"
     gene            complement(45953..>46600)
                     /locus_tag="PLD05_00430"
     CDS             complement(45953..>46600)
                     /locus_tag="PLD05_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOP75918.1"
                     /translation="LKKPHPKAEREHLEEELKTLQWVKQRLEENNPIRESEMTPEQQR
                     VTRAFRLLCEKPQMVVVNTADDERDFSRFRGMFPEYVPFFAAPLALELELSKMSPEER
                     EQFQAELGVGGADRGALIRTIMDVAGLHVFLTAGPKEVRAWLLRKGGTAVEAAEAIHT
                     DLAKGFIRAEVMSCQDLIRLGSEREMKAQNLVRHEHRDYVVQDDDILLIRFSVDR"
     assembly_gap    46601..46700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46986..48896)
                     /locus_tag="PLD05_00435"
     CDS             complement(46986..48896)
                     /locus_tag="PLD05_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75919.1"
                     /translation="MSDSAFQKRSDISIDAIREDLVAYLDGELSSEEAARIERLVEQD
                     TRIRSELQRLAETWDWLDVLDRPTVDEDFTRTTIEMVAQSAASEVVAQTQTKWKRRAI
                     VLGVAVLGTLLAVWGGWQLASFLWENPNKWLIEHYAVIQNQDYYRHIENVAFLRMLRD
                     SGLYTDEEEYLNPDDAQEVDEWRMFRPPGPPGPGQGQRGPAGAGEIPGEGGPTSNLRP
                     EPRDNRPSQVPRQPLSESSGEKLPSGSTAVVTSKPVSYPASSSVITLSDPAEIRKHIA
                     GLTDAEKVELRRKIERFQQLSPSEQARMKAIHEELMQAPDRDELIAVMQRYYEWFKLL
                     TPSQRGELETLEPAQRISWIQKTRNEMLTAAFSRALFNFPPERLRTLLQDSMRVPPEE
                     RLPPGDVAVLVRFVESLAAERGEELLAHLNSGEKQDLLRRLSRVTDPQQRRDFLAITW
                     FQWQLDHPEQASALTPQELEELRKQFSPGTRARLESLSTEEQLKRVAQWIRSYVFFKY
                     VMPRVWADMRRAVSEQELGRFVEENLTREEREKLLALPPDEMQRELTRRYLRWRYPGF
                     DTLGPWRGHGGERRGPRPGPGEREPPPGPGGSSDRDRGDGPPPQGPGANDVSTREPLR
                     PMPLLPPPGEVP"
     gene            complement(49004..49732)
                     /locus_tag="PLD05_00440"
     CDS             complement(49004..49732)
                     /locus_tag="PLD05_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP75920.1"
                     /translation="MTESKSSTQRAALRDPDVRLMLEVRNGNAAAFEELLLRYQSRVL
                     AVLENLLGNRHQAEDLTQEVFLRVYRSRHSYEPNAKFATWLFRIVNNVAMNALRSRCR
                     RREQTVPFQPGDSQHFDALDDAIQVSSGQIPARRLDKLEAREIVRMAMEGLNERQRLA
                     VLLCKFEGMSYADIAAAMKMSPKAVKSLLSRARTRLREILEPYFQSGQMPIGLGGTDD
                     VDSKRSTMIEDSQSDSQISLGGAG"
     gene            50352..54332
                     /locus_tag="PLD05_00445"
     CDS             50352..54332
                     /locus_tag="PLD05_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP75921.1"
                     /translation="MPSDPVELSLWCRGTLRVSVEGLGQRDRVTTTQPFARCGRLDTC
                     DVRLTAPGIPRRLLYLHATPQGLFALPLVELGRFAPLPSGWLVPGVPARVGPYRVNWE
                     WECQPPSGAALVSDPFKLGSLVEKGSAKQRWELDIYVDGHRVGRYELSRVLTLVGRHP
                     PSHLMMTSRSVSGCHCVIVAEENQLWVVDLCSANGIQLDGRAALAGWWPAGVPLQLGR
                     VTLVAKPMPSPVPIGTALAKAGTSAKSFQQPSSQVTSEPTGPVRPADVGTRSEPGRSA
                     SHPLPPDAGSLQEIPRADNSTGETLPTVFTPAGGVKKGAFDLEGPDSMDHPIEKGPGE
                     PSASAEPVILTGSGETTGLPEVIPDSQRPSGATPFDNLVVHQEFVDAEYPPVFVDTEI
                     TVEECMPENRLTAQAKDYVLCLFADESVDDHCLSVSSEKSELPHESTDRLEPHVAGDG
                     FPRKDAPHGQLTDAAAIEQKTQELLLKEQLLIAWAKTLDQMSEAILHLQEDLVAEEDR
                     FAEQQQLLAEQEKQLIVRQREIDARAEQLAILEQTLLQRERELEAREKALKEDREALV
                     RDTHNLAEERQTIERLRKEQQGRAEELVALHTSLAQREQEVREKQQLLAEHEASLKQK
                     AEQLSEEARRLAALEHELKCRTDALNAKEQDLFRRESEQREAAIRFADLELRLSQQQQ
                     MLLHLEQELESRKDELTRLEQGLLRQQKELSIRQQALSEQEQLLSSRCQELANLASHL
                     AEKEREIATQASNLARWEQNLQEWERDVKDRASRLPVVAPTEGAAESEIATQTTGNEQ
                     ELATPQRVESEVVEAQPAEGISGAEGFSSVTAESSVAELPGAVLATSIAPRVEIDRLR
                     EEDWPVIPLAKIHRRWRAPAPIAIGLVIAGLVACSVWWFCPREFITACRIEFPEDSPL
                     LYVRDGTRPRGVVEEFFPSMPAVAANDPRVVTRLGQIPEIPTLLTNGEKTWDQVLASA
                     KVSRSEGLPFTTMVITTRDPTKTQQAIERWGNVYLAMLAESFQEKVSPALAKAKQRWQ
                     ITSQQLKEHQASINDLKQRYQVEHPRQLELQAHESLHMAEVTQKEIDQLQAEITAQKQ
                     RLEQVKAVADRPEQAISEEEVMAAVAERLAQDGLTVETSDPGADAVNPDPGIADEAEA
                     NSQQPPDGSNAPRLNAPDPGSRYEIVRQLVRKELSERKRQDAVLTVQVLTAELDRLQA
                     RLQKAMEQKAQYEATAGALLEAAKQWNNLEVETAKLENEHQQLTTQIDILEKALAQSR
                     IAPHLEIWSSQQRPWMPFAAAALAGGGAMIPFGLWGLLRSLRTVRVFPPAAIGYQSKY
                     PHPTPD"
     gene            54491..55951
                     /locus_tag="PLD05_00450"
     CDS             54491..55951
                     /locus_tag="PLD05_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP75922.1"
                     /translation="MFGKVSRRRVLAGLTSVVAVPTLVRSSALGLAGLAPASERVTLG
                     LIGCGGHGIGWNLAMVFRHNDAQVIAVCDVDQRHLAAGKQRVDEHYRNVVGEQYRECA
                     AYRDFRDLINRRDIVAIMNVTPDHWHVLPALMASECGKDVLCEKPLTLFVEEGKVLCQ
                     TVAKNKTVFQTASENRSIDVYIQLVSLVRAGVIGKLKHIEVRLPMGNTNTRVVGEAKD
                     MFYQSSPQEVPAYLDYDMWLGQAPWMPYIPARLHGNFRWNLAFSGGVLTDWGAHMIDL
                     AQWGHNTERTGPVEVEGRGDFPPRDAVFNTAPTFDLLYRYSDGVTMRVSAGQGDLDPQ
                     QVKPDTPLVGRTPSPGIRFEGTDGWIESHNWRGVLRASRSEMLKVEVDPKALGIYIPG
                     EVVARDSGRGGEHRNFLDCIKSRQPCYAPAEVGHRTITIAHIGNIAMLLGRKLRWNPD
                     KEEFLGDDEANAMLTRKQREPWTMANVTSWIKSGNS"
     gene            56161..58170
                     /locus_tag="PLD05_00455"
     CDS             56161..58170
                     /locus_tag="PLD05_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75923.1"
                     /translation="MRAFSWAFSLLVLLEFGCLVTDGHSGLTAQEVPPTTPSLSTTAS
                     QPASPEASRLQVSIGQAENSPAGAVSPKDTALRIESPNNPVSESAALPSESPPTSVAP
                     RPDPKTNTAPLTVEAAAFNGVTPGVSHVNDLTRQWGPPKEAVTKGDSVYYLYEIPPFD
                     RIEVHIQNDKVEAIIIRLDKTYPANLVAEHLELSQFRPVLVSNPQGEVLGQTFPERGV
                     LFSFAPAPEPGKSSNLVTEIILSEVTAEPFILRAETFWQTEPDSAIRDLEQALKFDNR
                     AHRAWWLLARIQKELGSLKEALASAQNAVNLDPASLHYRLTLAEILDEVGQDNLAIQQ
                     LEQVVAASDQRPHIKARALCNLGNIYQSLPQPDYKKALDYHYEAVKIAQPLRSDVHPA
                     IRVAAKEVLVDAHLGAALDIAWGPWEQKEAVVPRWLERAANLAQDLVNTEQQSADKLF
                     RVSTTALAASVAMPRVIGPLPWIEALDQAYQRLEAEASEVRRARLRWNYGVALYDAVQ
                     CFQVLGEQELAQQYGKKAAEALETGLQGRSPKGSDLYLLGRLYFRMGAVDAIQNKDHK
                     QAVTYFDKAIPLLRQAAGDIPETSIGRLGETFVSMGVSYWETGDRDQAIALTEEGLQW
                     MEKAVQLGLLANSALDVPRANLQTMRQARQRLAERTSSDTAATQR"
     gene            58568..59017
                     /locus_tag="PLD05_00460"
     CDS             58568..59017
                     /locus_tag="PLD05_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOP75924.1"
                     /translation="MTRTLVPWTTSPSRLFESLRREMNELMDQFFGSDGGTDLTLWFA
                     PRINLAETDQAFEVTVDLPGMKPEDFSVELKEGQLWITGERRQETEEKGKTYHRVERQ
                     YGRFQRVIPLPAAVNPEKIEAQYKDGVLHITLPKDETAQPKRIPVKT"
     gene            60044..>60522
                     /locus_tag="PLD05_00465"
     CDS             60044..>60522
                     /locus_tag="PLD05_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP75925.1"
                     /translation="MTFWLAYLSNALVSAGNVLLCRYADYVNFLGADEYHLGWIIGFG
                     MVGSLFMRFALGSGIDRFGARRVWLLTILATALGCLIHLGITDYRGIAIYLARVFYII
                     SLAGVFSASMTLIAARAPRERLAEVVGIYGSAGFAGVLLGAPLADLLFPAGITSMGEA
                     "
BASE COUNT        13493 a        16437 c        17169 g        13323 t
ORIGIN      
        1 cagaaaggcc tatttcacgt cgagagggta gcttccagag acaaaacaag ggtttttttg
       61 ggtaggttta aggccctaca aacgcccgga tgaggacgac cacgcccaag gtcatgcgaa
      121 cacataggcg gcttgtgata agcgataatc tcaagggagc taataaactg aagatgggat
      181 ccaagtaaaa cgggagcgtg tagacggtgg ctagtttaag caaagattca tccggatggc
      241 gtgtacgttt ttaccaagac ggtgtcctaa agacactacg gcttggcaac attcccaaga
      301 aagatgccgt cacagtggcc aacatggttg agcggctact aacggccaag cggctaggga
      361 ttcccctgga ttcacaaacc gcgggatggt tgacgcgtat cagcgatgac ctccacgatc
      421 ggctagcccg cgccggccta gtcgaacccc ggcaacgggc gactttgggc gatttgctgg
      481 agcgattctt cgcggcccga ctggacgtaa aaccggcaac ccgcgtcacg tgggaaaacg
      541 tccgtcgtga cctcctggcc tactacggcc ctacgtgtaa gcttgccgac atcgaccgag
      601 accgcgcgga ggcgtttcgg gtgtaccttg tcaaccgaaa gctagcgtcg gcgaccatta
      661 gccggagact ccaaaccgcg cgccagtttt tcacctgggc cgttgataat ggactgatcg
      721 acaccaaccc cttcgccaag gtcacacaca cggcagggaa tccccgggaa cggcggcgat
      781 atgtcacgct gcaagagacg caacgactga tcgacgcggc ccccaattgg atttggcgga
      841 cgattattgc gctctgtcgg ttcggaggtc tacgctgccc tagcgaagtg ctgtcgttgc
      901 gttgggagga tgtggatttg ccgaatggcc ggatgatcgt gacgtccccc aagaccgaac
      961 accatccggg aggtggccga agggtagtac cgatcttcgt ccgactgcgg ccctacctgg
     1021 aagaagcttg ggaccatgcg gagggacata ctcacgtcat ccccgaaaac ctctacctta
     1081 aggcaagccg cggcccacgt ggttgggtga attgcaacct ccggacacaa tttgaacgca
     1141 tcatccggcg agcgggcctg gaaccgtggc cccggttgtt ccatagcttg cgggcgtcat
     1201 gcgaaaccga tttggcggca gagtatccca tcgccaccgt ttgccgatgg attggaaaca
     1261 cggttgccat tgcggccaag cactacgtgg aaccgcttga tgagcacttt caacgggcag
     1321 caggcttggc catactagcg gccatgcaca cgccggaaat gacgcgcact gacgagaagc
     1381 cgaatcgtga aaaccccgtt ttttccgggt tttgcgctat cgcgccgact agtgcaaata
     1441 ccccaatggg cgatgcggga ctcgaacctg cgacccccag cttgtcgagc tggtgctcta
     1501 accaactgag ctaatcgccc aacacactat attaatatcg gtcgccgccg atttggcaag
     1561 ccagggtcct tttggcccag cgctcctcgc aacgaccggt cgcatggcga caaccctttt
     1621 gggtggtttc aaagttgacc cgattcggga ttcaccttgg gatcacggtt aatccattga
     1681 caggcatgcc acaacgtgtg acatggctca ccgttacgaa ttcgcgattt cattccggca
     1741 gccgtctcgc aataaaagcg ctttaacccg ccacgagggg cgcaccgtga ctgccgtgtg
     1801 gtcaaagaca tgggctgagc cgtagcctcg gctcggcggt ggctgccaga tcggttgtgg
     1861 gcactttaca atgggatttc ccaccggagc tcgagagaaa agcaagcggt tcggggaagt
     1921 acgcagtggt ttaaacattt tttgccagga ctgtttgata tagagtctta tcgatgattc
     1981 caccacgttc acacttccag gcgggctaca attttttggg cttgcccgac gagttggccg
     2041 acccccaacg cgctcaggtg tggatcctgc cggtccctta cgaagcgacg accacatacg
     2101 gagcgggcac ccgaaatggg ccgacggcca tcatcatggc ctccaggcag gtggagctgt
     2161 acgattatcg acgaggctgt gaaccggcga gcgaactagg tttcgcgacg ttgcctccat
     2221 tggagattca cagtggatcg cccaccgcga tgatttcccg gatcgaggaa gtcgtcttac
     2281 atatttttgg gggagatccc caaccgcgat ttctcgtagc tctcggtgga gaacacagca
     2341 tttccgcggg gattgcgtcg gcggctgcaa ggatctttga cccccacgag attgtggccg
     2401 ttcaaatcga cgcccatgcc gaccttcgcg acgagttcga gggctctccc tacaatcatg
     2461 cgtgtgcggc gaggagaatc ctcaactgtc ttcccctgat gcagatcggc attcgcaaca
     2521 tctccgagga ggaagaccgt tttcgaagta ctgcccatcg gcttatcacg atcttcgccg
     2581 acgagtggac ggaggccgct catgagcggt tggccgagtt cgtccgcaac aaactggtct
     2641 atttgacgat tgacctcgat ggtcttgatc cgtcgatcat gccggccgtg ggaacgccag
     2701 aacccggtgg tctttcgtgg caacaaatcc aggaggttat tcgcattatt gcgcaaaacg
     2761 cccgcggtgt tcctatcatg gacgtcgttg agttagctcc catccccggc ttgattgctc
     2821 cggacttttt ggccgccaag ctcatctact gggcagcgtc agacttcttg cttcgggaag
     2881 agagaaaggc tgccctttga acattagaag agccaaaatg atgagcgatt cgtgcccgat
     2941 tcccgcaaaa cgaacccatt ttgggccttt gcgcagattc gccgagtccc ctagtctttt
     3001 aaaagagggt ccggcgatga ccacatgctt gagatgctct gaaatgatga gtaagcgttc
     3061 ccagggcgga taggcaactt cttgggactc agggggttgc acggtaagag tctacccata
     3121 atctcccctc gcccgtttgg cgggagaggg gtcggcggtg agggtagctc gaatcgctgc
     3181 cggttttctt aattgattcc gacgttcgct tgccctcacc ccaaccctct cccggaggga
     3241 gagggggacc ttttgggata ggctctgacg actttgtggg ctaggcgaag aggagggacg
     3301 gcgtccccgc cgtccggtga tttggtcatt gaggccggtg gccctgccat tacgggtgtt
     3361 ttttgggaag atcctccgtt ccctctgaag ggtcacaatg acgtcttgcc caagtgacac
     3421 ccatatggag tgtttgggca aggcgcgacg tccttttcac agaagaacgt ctgccgccga
     3481 ttcttttcct gggtgacgtg atagagcagc ccccccaatg accaaactta cgaacctcgc
     3541 catggcgaga cactgccaag tccaagccgt tgggtaaaaa tcgggttatg cctgcggaat
     3601 cggggcatca cttcatgcgc gcacagcgag tggcccactt tttccaaagg tccggcgatt
     3661 cgaggaattt gataaacacc ccaccgatga caggccgggc tcggaatccc cgtagcttcg
     3721 catcgtgggt ccagtaccaa tcggtcagcg ggacgcggtc gggcgtgtga tcaatgaaac
     3781 ggtagagcgg gttcatgagg gcgtcaaagt cttctcgaga atccgccaat gtggccgtcc
     3841 aaaccgtcca gtccgtcttc gtaaagaggg cacggttatc aaggggcagc ccataaggct
     3901 ggagttgctt cttgtagaac gcgatttcct tttggcggac cacagatggg aagatgttga
     3961 ggccgaatag tttgtcccaa acgaggttgt atttctggct ccatgtcccc ggcttgtcga
     4021 aggccagtcg atagtggtca ccatcgtcag cggccgcgat ccatttttgg gcgaactcct
     4081 cggcaagctt gcgatacttg tttgcctcct cgatttttcc actcatgtga cacatttccg
     4141 cgtaacatgc cagagcaatg gtggctttta tagaaagatt gcaattgtgg gcaagggggc
     4201 cggtgaaatc atccgtgcag agctgatttt caggatcgaa acctttctcc tccagataat
     4261 gtgcccattg ttcccacaat tttgtatatc ggtcggcgaa ggccgtgttg ccgtcgaggc
     4321 gggaaatggc gtaggcgaga atgagcaagt tcgccgattc ttcgaccggc atttggtgtt
     4381 cctcgctggt ctctccgcca ccgtaaacct gaccattggc caggggatag gttcccaaat
     4441 cgtgtggcgc aaaagggaac ttccatcgcg ggctagcggc gtaatcgagg acggggacga
     4501 ccgtggcttt cagcaactcg ttgttaaaaa gcatgaagat gggtgctgcg ggataaatca
     4561 catccacggt cccgatgcag ccattgctga agcattcctt ggagaagtac atcggttcgc
     4621 cgtgtggccc cagggtcagt ttgtgcgcac ccagggtatg ccggtaggaa atcgcaatca
     4681 gattggcata gcccacgcca ccgaggcttc gagcgtccgc gagaagttcc tcatcaaagg
     4741 cctggcatcg agccatgagg tctggtagct cttcctcggc cgcggccagg gcttccgaaa
     4801 tgtctgcgta tcgctgacgc caatagggtc gcaagcgttg ccccaggtac tcgatggaaa
     4861 caatatcgtc gtaagccagc aagatgtgcc atttgagtga ttcgtcgcga cttaccttgg
     4921 ccgagtgcat acataccaac acaggccatc ggtcctggac tgcccgaggt ttgtttccgt
     4981 cgtcacggcg cgggattcgc ccggtgttac aaaaatgatt acgggcggtc tcgtgatcgg
     5041 ctacaaccgt gttcgtttgg ggatggcgca cggcggcgac ataggcgtag ccccaatcga
     5101 tccttagatt gtcgcccttt ttcccgagga tcggttgctc cgttgtaccg atgcgcatcc
     5161 aatccaggtg ctcggtctgg ccgcggtgcc aggtcacaag ctgattcggc tcgttgacgg
     5221 ccaactcgcc tgtggcgtca aaatagagat ccacgatccg tggtttgcca tcgagtgagc
     5281 gaacatccca aataagatag gtgacaggcc gggaaaggag tgccatatcg tgcggcagaa
     5341 cgggtgtcaa aaaggttaga cggacttcga cctcttgggt tcgccacaaa taaatcgtgc
     5401 gggttggcgt gacctctagc gaagtttgtt ccaaaggagg aatatccgcc ggtgccatac
     5461 ccataaggcg atacggcctc ccgtcaatgc ggatgaggct gcacaaggcg tggattcttc
     5521 ccgtccagtg gcgtggccag tccgccgcga gctggtcggt aaatgaccaa atgctaaagt
     5581 atgggtcgtg tacaaccagc ggcactgccg gtggccgacc tgccggacca tcgttagccc
     5641 acacccccgt ctgccgtccg ctaatgaggc taatgaacag cagaacagtg gtagaagcaa
     5701 ttagccttgt catactggac atgttgcacc tcactgcttc gtccaaaagt acccgagagc
     5761 cgcgaaccac cattatggac tttgaaatga ttcctgacca cccgcgcgga gtttggtgcc
     5821 cccacttggt atggcaagat acgtaaagct aggctcgctt tggcaagaaa aacaattcca
     5881 ggccacaagt tgacaagccc ccgcgggctt gctatgctga caatcgaaaa tgattgtgcg
     5941 aatgtccaca atcgcacaga tatccccgcc ttggcttacc ctccgcgctc ggggagttca
     6001 aaatgcatcg cacgatccca ccggtatccg gatattggcg gttaacgtca tgccaaggga
     6061 tcgattctcg gtcgcaaggg gctgctcgtt tgccatgggc ggccatcacg ttgcgggcta
     6121 ttaacctcga gcaattcagc acgaagactc ccgtcggctt acggctttct cggaaaggtg
     6181 actcttatga ataaaactgt aacgagtgtc ttgtgcgcgg ttattttgtg gggcggcttg
     6241 ggatttgcgg cggacccaat cagcctatat gaaagtccca caccagccca gcccaacggg
     6301 gagctggacc gaatcgtctt cgccaagttg caaacgctgg gcgtccaacc gatattatgc
     6361 tcggatgccg tcttcattcg gcgcgcgttc ctggatatga cggggaccat tcctacggcc
     6421 caggaggtcc gcgagttttt ggatgatcag actcccgaca agcgtgcccg gctagttgac
     6481 cggctgatgg agcggaagga gtttctcgac tactggtcta tgaagtgggg cgacgcgctg
     6541 cgaataaaag cggagattcc ggtgaacttg tggccaaacg gggcaagggc ctttcatcgc
     6601 tatatatggg cgtcgctggc atcgaacaaa ccttacgacc aatttgtgcg ggagatgctc
     6661 acggccagcg gcagcaattt ccgagatgga gcggtgaact tttatcgtgc ggtgcaggac
     6721 cgcagcccca cgggaattgc ctcaacggtg gcactaacgc ttatgggggt acggacggac
     6781 cattggccca aggaacggtt agaaggtatg gccgtcttct tctcccagat cgcctataag
     6841 cccaccagcg agtggaagga gcaggtcgtc ttctgggatc cctggggcct tcgcttggtg
     6901 ccgacaaatt ttgttcccgg acgagaggcg ccggggacag ctccgcggaa atcggaagac
     6961 cctatcgtga ccccgacccc ggcggatatc agtcaggagc cgctcgtggg tgtctttccc
     7021 gatggtacta aggtcacaat cccggcagac cgggacccgc gggaggtgtt tgccgattgg
     7081 ctcattcggc cagagaatcc ctggtttact cgctgcattg tcaaccgcat ttgggtgtgg
     7141 atcatgggcc gcgggattat tcacgagccg gatgacatcc gtgacgataa cccgcccagc
     7201 aatccggagc ttttgtccta tctggaaaag gagttgatcc aaagtgggta caacctgagg
     7261 cacctttaca aggtgattct cacgtccagc acgtatcaat tgacgtcgat ccctcgcaaa
     7321 ccgtcgccgg aggtcgaggc gaattttggg tactatgtac cgcgaagact cgatgcggaa
     7381 atcctgatcg acgccctgaa caagatcacg ggagcaaccg atctttacac gagcgccatc
     7441 cctgagcctt atacatacat tccgcgagat ctcacggcgg tgtcgttggc cgacggaagt
     7501 gtcatcagtc cttttctgac cctattcgga cggtcagctc gttccacggg ggccttcaat
     7561 gagcgctcca ataagccaac gccgtcccaa tggttgtata tgctgaattc gagccaggtt
     7621 tataacaaga ttgctaccag ccagaagtta ggagagttgt tccgttctgg ccgcccggtg
     7681 ccggaactga tcgaggaggt ttacctgact gtgttgtcgc gacgtccgtc agaagaggaa
     7741 atgaccattg cacgagaata tattgaaaaa tcgcgatcga gaggcgaagc tgcgatggat
     7801 gttgtctggg cattaattaa caccgaagaa ttcctatatc gacattgacg atacagaggg
     7861 aggaccaagc catgaacaag aacaatcatt acaatgagcc gaaggtctct cggcgggaga
     7921 tactgcagca agggatctat ggtgccgggg gggtactcct cggaaagtca ttactctcgc
     7981 aagtgcgggc acaagaggcg gcaccggccc cgtcgactcc tccgaaaatc gaaccaaagg
     8041 ccaagtcggt cattcagatc ttcctttggg gaggtatgtc ccataatgac acgtgggacc
     8101 ccaaaccgaa gtccggcagg gattacctgg gcgagtttac agaggtcatt gacaccaacg
     8161 tcccgggcat tcaattgagt tctctctttc ccaagttggc acgtcaggcg gataagtttt
     8221 cacttatccg gagcatgacg catggcaaca atgggcacga aacggctgca tacatcatgc
     8281 agacgggcca tttgccaggc gagcggttgg cctatcccag catcggggcg gtttttgctt
     8341 acttcaaggg aaaggaatac aagggaatca ttccgcccta cgtggtcctg acacggtcac
     8401 cgggacgctt ttcggaggag ggtttcctgg ggccgaactt caagtctttt gccaccgggg
     8461 gcgatcccaa tgcaacccgg tttgaagttg aaggcgtcgt ggcacgcggt atcacagatg
     8521 atcgccaaaa ggcgcgccgc gatctactcg aaaaattgaa cacgttcggt cgattagtcg
     8581 ttgaccatcc ggagatcgcg ttggccgaag agaataagaa agcggcctat gagctgattt
     8641 taggaccagg tcgagatgtg tttgatctga gcaaagagga tcccaagctg cgcgacgcct
     8701 acggacgtca taccttcggc caaagctgtc ttgtggcccg gcgattggtc gaggcaggcg
     8761 ttccgtacat tgtcatcaac tatcccggtg ggtgggacac gcacagcaat cacttcacaa
     8821 ccatgcggcg gcagtgccca cagctcgatg aaggcttggc catgctgctc caagacctgc
     8881 acgaccgagg cctactggag acaaccttgg tctggtgtac gggcgagttt gggcggacgc
     8941 ccaaggtcga ttggcaacca ccatggaacg gcggacgaaa tcattacgga aaggtattta
     9001 ctgtgttggt tgccggcggc ggcttcaagg gcggtcgagt cgttggagcc tccaacgaat
     9061 tagcggaaga ggtcgccgaa cggcccgtct atccggtcga tcttttgggg agcatttacg
     9121 ccttggcggg aatcgattac acggccaagc tcccgcaccc cgaaggttta gaggcccgtg
     9181 tcctgcccga gccgggtgac cgtgtcaaat cgaatgggct gcttaccgag atcatctaag
     9241 cccatcgccc accgagcttc agtggtgacg aagccggacg gggtctaccc cgcgagggtc
     9301 gatggcttgg agcctcgaaa tacacaagcg gcggcgtttc gtaactccag tgacatgcga
     9361 tggagaaggg aacccacttt atgaaaagat atagtgttct tgttatcatt gtggcgatgg
     9421 ccagcctggc gttcggcacc gcagcttttg gtcaggcata tattggattc gtttaccccg
     9481 ctggggccca gcaggggacg accacgcaaa ttcgcttggg cggacagcgg atcgacatcc
     9541 cagtgggggc catcgttacg ggtaccggtg tcgaagccaa gctcgtcgag taccgctggt
     9601 acctcgataa tcagcaagtg gccgtgatgc gggaacagct tcaacaactc cagcgcgtgg
     9661 ccaaggaaaa agagaaaaat aatgaacctc tcgacgacgc aacccgccag ttgatggaac
     9721 gcatccagga tcgcatcaat gactggcaga atttcccagc caatcgagcg atcgtcaact
     9781 tagcaatcgt ggaagtgact gttgccccgg atgccgaacc tgggccacga gaaattcggc
     9841 tggtgacaac gcaaggggta acgaatcccc ttccttttta tgtgggtcaa tttccggagt
     9901 acgtccgtaa acccatcaag gcctgtcagt ttccagtact gggcaatgaa caggccgccg
     9961 agcggcgtcg tccgcccgag gaaaaagaag tcagggttac cattccctgc gtcgtcaatg
    10021 gacaggtggc accgggtttt gtgaatgctt accggtttga agcccgtaaa ggtcagcggc
    10081 tcctcatcaa agcttacaca cgggatctaa ttccttacat ggcggatgcc gttcccggct
    10141 ggttccagtc gatgctcacg ctttatgacg cacagggcca ggaagttgcg ttcagcgatg
    10201 gctttcggtt tcatcccgat cctgtcatct attacgaagt accgcaggac ggcgagtacg
    10261 tcttgaccat ttcggatgcc cttttccgcg gacgagaaga ttttgtttat cgcatctcaa
    10321 ttgcggagtc cccatttatc acgagcgttt tccccttagg tcggaagttg ggcgaatcca
    10381 tcacccccga ggtaaaaggt tggaatatgg aaggggcgga attaaggcta cctcccgcag
    10441 atgcgggatc tggcgtttac tttgtgacgg cggtcaagaa cggccttgtc tccaatcgct
    10501 ggccgtttgc cgtggatacc ctccccgaga ttatggaaaa agagcccaac aacaagcccg
    10561 cggaggccga gaaagtttcg ctccctgtga ttatcaatgg gcgaatcgat cagccgggag
    10621 atttggatgt cttctgtgtt gaggggaaag cggggcaaac attagtggcc gaggtcacgg
    10681 ctcggcggct cgattccccg ttagattccg ttctccgaat cacggatgcc agcggtaagc
    10741 cgatcgcgct gaatgacgat tatgaggatc ctggaatggg gcaaatgacg tatcacgcgg
    10801 attcctatat tatgatcccg ctacctgctg atggcgccta ctatgtctat cttggtgaca
    10861 ccacgcggaa aggtggccca gaatatgcgt atcgcctccg actgagcgag ccgcgtcccg
    10921 atttcgcgtt gcgcgttgtt ccctctgccg tggccgtgcg tagtgcaagt gccaacaccg
    10981 tcagggttta tgccattcgc aaagacggct ttgccgggga cattcaagtg tctctcaaag
    11041 atccaccgga aggatttgcc aatgcgcccg tcgttttaag ggccaatcag gagatggtcg
    11101 gacttaccat aaagacgtct ttgaaagatt cagatggggc gattccacta cgggttgagg
    11161 ggcgggccaa agtcggcgat caggaaatcg tccatgaggc cgtcgccgca gaggatcgca
    11221 tgcaggcctt cttgtggcgg cacttgttgc ctgtggcgga tttgagggtc cgggtgtatg
    11281 accccaatta ccagccgcaa cccgcacgaa ctccgccaga agtgcctccg gaaatggtgg
    11341 aggctgcccg caaggcggct caagaagcag gccgggcacc cttcagtaag gcccaggtcg
    11401 ccggtcgcgt gaggatgctc aatctccttt accaaaatgg gttcctcacc gatgatttct
    11461 acctcgtgaa aatggcagaa tgtgccgccg cgcaggattg atatcgagcg gagctgacaa
    11521 agcaattcct cgctgaggcc gaattcgcac aaaagaagcg gccttcggat gaagaaacca
    11581 ccctcgaggg cgcataccct cacgggtgga gttttgtcag gaatcgaacc cggacgggtt
    11641 ttcttccggc caaagggagt cttgcgagtc gaaaaaccgc agctcggcga tcctttcggt
    11701 agtggtgaga caccggcccc gagccgctgg attaccaggt caacgggtca ggatggcgaa
    11761 gaccggaaag tgatccgagg gggttcgtcc ctggcgggcc gtccgatcga tgccagcctc
    11821 aaggactttc caatcccgcg aacacccaat ccaatcgatg cgtgccccct tagtattttc
    11881 ggcccgaaag cccgaaaatg taccttcttc ttcgccccgc tcaggatgag ccgcccgaaa
    11941 gctatcgaca atcggagagg gttgaccctc ggtgtctccg aatagcaatt gatagggccg
    12001 acttccttcc gcggcattga aatcaccggt cacaatgagg gagcaatctt cgcccatcgt
    12061 gagcagcttt ttgcggagga gccgggccga ctcttgccga gcctgttcac cccggtgatc
    12121 aaagtgtgtg ttgaaccaaa gaatgggccg gaggttaggc gctttacggt cccgcagttt
    12181 gacccaggtg accatccggg gcaaggcgct atcccaactc ttgctccctg gaacatccgg
    12241 tgttgtgctg agccagaagt gaccagattc gagcacatca aaacgatctt ttcgccaata
    12301 gatcgccatg atttctccct gatctttgcc atcttcccga ccaacaccca ggacgccata
    12361 accgggaagc ttttccgcga gatagtcacg ttgaaaggcc agggtctctt gggttcccag
    12421 caaatcgggc tggaaggcca ggatggtttc taccaggaat tcttttcggc ggtcccagtg
    12481 gttttcccca tcaggagccg tgccataacg gatgttaaaa ctcatgaccc ggacgggggg
    12541 agcctcggca acaacgggta ctccacttaa ggccaagaga cccaggccca acaaaaccgg
    12601 taccaagacg tgactcagat ggtttctcat tcgctggact cccacacaag gacatttttc
    12661 caaagagatg tcgttttcca ccgagctgcg ctgaattata ctgccgtaaa ggcgtcgcaa
    12721 cgagtagcac aaagtcgata gggccgaagg cacgcatagt cgagttcggc gacgtgaacc
    12781 gagtgtgatt gattccgtca ggccgtaaga tagccgcacg ggggtggcat tccgattgtt
    12841 tgttacgagt cgtcccattg gcgggaatac attcctgagt caatcggttt cagtggaccc
    12901 gagtttcaag acgaaactat tgggtgcgtg gtttcttttt gtacctgcct cgcgaagatg
    12961 tcccttcttg cgggttagct tttggtggtg agaacgcaac tttcatgata cggaaaaggc
    13021 atgacacgtt gtttgctctt aatcaatgag ccgggtagtg ggccatggaa catggcgatg
    13081 gacgagttct gcgcgaccca tgcggaaaga gaagacgtcc ccattttgcg gctctaccgg
    13141 tggagagaac caaccctctc gttgggatat tttcaacgct atcaagacta tcaacggcat
    13201 cgcctaaacg ggctttgccc tgtcgttcgc cgtcccagcg gtggaggggc catcctccac
    13261 gatttggaat ggacctacag tttggctttt cccaccagcc gtgtgacaga ccgcgatcgg
    13321 ctttcgttct atcgtgcggt tcaccagacg attttgacct ggctcgactc gctgggacta
    13381 aagggcgaaa tcattcagga gcaaccaccc ccgtgtaagt cgaataacac cgattgtgcg
    13441 ttcctgtgtt ttcagcgtcg ggcagttggt gatgttgtgg ttcccctttc cccggagatg
    13501 cacacactag tatcggctgg ggccacaggg gaaccagtgc ccacggacgt caaaatagtg
    13561 ggtagtgccc agcggcgcta caagaacacc gtccttcagc acggcagcat cttattgggc
    13621 cgatcagtgc ttaccccgga attgcccggc gtgatggaaa ttctggccag caaggggatc
    13681 caactgaccc atgacgaaat aagttaccat tttgtttcac actggcccca aacagtggct
    13741 cgtgcgctgg gatggcgatt gcaaccttgg acgctttcat tgaccgattc gcccaaattt
    13801 gctgaactcg aagcaaaata cctatcccgc gcttggacgt gcaaatcaca agcgtaagca
    13861 catcgggaat caacggggaa atctcttgac cgaccgctca ctccaaggaa attcggtctt
    13921 ctccctggga agcgatcgtc ttgatctgtt cgccatcaat gacaatttga tacgacacgc
    13981 ctgcccgagc ctgaatgacg gcctcatttc cctcgatctt gacgggaaaa ggcgcaacga
    14041 cccgatggtt gtcaccaata gcgacggcca atcggtgcgg tcgccccttg atggccgaac
    14101 gaagaagggc aagggcacag gggttgataa aaggcccctg tcttcgctga tagcgaaggt
    14161 ggaatgaatc cggcaataga ccggcatagt ctccctcctc cctggggact tgcatttgtt
    14221 cgccagcgat caagatgccc tcggcaagat gacgccaatc cagggtattg tcgtaagggg
    14281 cgagctccgt aagcgcgtaa gcatagacca gaccgcacca ttgtacgggg agaccgatcc
    14341 aattcggcgc ccgccaattt gtagccccat aaacgggaat cgtggcataa agcatgatag
    14401 gatgttctgc ccaaagataa acaaacggaa ttccactcaa ggcccagcgt cgggcttcat
    14461 caaggtaatc ttgacgcttc gtcagctcat atccccgaac gtaggctgaa acaaggtgcg
    14521 ccgaagccaa aatgtcgggc gtatgcaaag agagttccca cgtctgtgcc ccacgaggga
    14581 cacgaaaacg cttcatatag tccagcgtct tgagaccggc ctcaagggcc tgcttgtctc
    14641 cggtaagttt ggcatactcc agcaacacgg cggcgggacg tgcacagtag ccgctcgcgg
    14701 tgtcctcata atgtcctttt aagtattttc cagaataccg aaaggaacca tcaggctgtt
    14761 gttgcgccat gagccccctg gcctggttcc gcatgtgcgc gagccactcg gcggcacggc
    14821 ccgtcaagaa ccatgcactg tcgtccgaga ggtgaccacc gccgggaaca agatggggta
    14881 catcaggcaa tgtccccgat agccgccata tcgtagaaac catatcgaca tgaaagcgac
    14941 gagcccagtt cggttctacg cagtgtcccc agccctgctc gttacgaata ggaccatcag
    15001 tcaacgccca caggcacaat tgaaattgct ccgattcgct ccggggcggt tcaggcaaag
    15061 gcggtaatcc ccaccgcttg gttgcccaaa gaatcatgtc ctcaaccatc ccttgcgcga
    15121 tgcgcagacg ggcctcaatg tgatgccctc gaagcgacat gcgatgctct tgggtgccat
    15181 caaagacatt aggagtggca taaatgggct gcaatcccat gtcattccat gtgagtccca
    15241 ccgacgcccg atcggtgaca aaagccatta ggggcatggt cacatttagc cgatcagggg
    15301 catatcggat gtgctcttct gtttcgatat ccaacgtgga agaactcttt tcaccctttc
    15361 ccagatactc caggccggca aagacgcctt gctccaagag accaaatgcc cgcaccaccg
    15421 ggccttcgat ctccgagttt tcatctgtgg cctccagatt taggatgagt tcgtccttgt
    15481 ccacaacaat cgttccttga acccctttcc cttgaacttg gacaccattg gggatggggg
    15541 tggcctgtag cgggggaatt gtcgcaatca ggccggcgcc gttgggatca gccggggcca
    15601 caatgggtgc cagggctgct agtaccgtct ccccgcgacg gactcgcaaa acattgtctt
    15661 tagccgccag ctccacaagc acggcggtgt ccccacctgt ggcgatggcc atccattcgg
    15721 ccggggcaga atcggaatac accacgactg aatgccgcag gtcggtggtg aaatcgggga
    15781 gctgcaaatc cagggttacc aattccactg gccgttgtcc gtcaaactta cggatgatgt
    15841 cgatggcctg accggccggg ataacaagcg agtgctgctc catccgtaca gtgacaggag
    15901 cattgcttgc attctgtata tggaactgca caccctcgtc tgtcctttcg atgaattcga
    15961 tattgatggg acatgtttgt aactctgtca actgaatgct ttcgatctca tagatccccg
    16021 gtgccgtgcc cggatcaatt cgcaatccgc ggaggccacc gggtgtgtcg attcgaaccg
    16081 tgtagctgtg ccactctcca tcggcaatga ggaaaaaatg ccgggattta tcctctcccc
    16141 aagttagtga cttttgacta gtccagtaga tggccccacc gccacgcacc gtggttcgta
    16201 aacgaaggga gacctcatag gcctcgcccg gaagattcat cgccttaacg atgtacgggt
    16261 catgcccggt gctctcaacc accagtcgcc cattttccac cgcaattcga cattggctga
    16321 cgggctcaaa gccttgcgtt ccttctccaa agcccagcgt tagggactgc tttcgtgtca
    16381 cggccttcgg aaagatcccc gacgcttccg ccaatacatc gcgggcgcat cccaataggg
    16441 aaagaaatgc gagcgcatga accaccaacc gcatggcaat ctcccagtag gtttgaacga
    16501 ggtaaacctt tatgtgcacg aagcatttat tttggcccac acgttaacat tatgaaacat
    16561 ggggggacat aggggcttag acagtttgaa aatactaaca cggtccctgg gctcgccgga
    16621 cgcgaattct cattgccagc ctcgtcgcaa gttattcatc agcaataaga tatgactaga
    16681 acgcttattt tgggttgtct tttttggttt tggcataaaa catgcgtcag gggataacac
    16741 cagacgtctt cccatcgatg ggaatgggca agcttgtcag gtcggcctgc ggtgggtcgg
    16801 ccaaggagtg tcttgtgtcg gttcctcgag tctctagcat aaggagcgac agagtgaaac
    16861 gtgtattgtc tcgtgtttgg cgggaggatg aaggtgtttt gaccttcgaa tggatcctcc
    16921 tcatcaccgt cctggtgatc ggtgttgtcg gcggtctcag cgcggttcgc gatgcggtca
    16981 tcaccgagct gggcgacgtg gtcgaagcga tgatctcgct cgatcagtcc tactacatcg
    17041 cccatccgtg ggatgtccaa gttcctgact gcatcacgga tggcgccagc gactcgtctt
    17101 acaccgactc tgctggaatg gctcagggtc gtctaaagcc ttgggacatt gctcaggacc
    17161 agggagcctt tgagggctgc agcgacgctg tggaaaatcc acgccggtga tctgcccacg
    17221 taacatcgtc tgacagcatt tccagacatc ggtctcggaa gtgcgcggct ccgcggaatc
    17281 atcttccgcg gagccttttt tatatgggat atggtgatgg atgaccaagc gtaaccacgc
    17341 aatcgcactt cgaaaaaatc aaagttcccc agtaccagct cgttcaaaat ggcatgaaat
    17401 ctgcacatca agcaaagaca aacatcgcgg ggcaaaagtt actcgtcgat ttaaacacaa
    17461 ctcttgtgga aaaaggtgag gaccgtctat gaaagccgtc ttccaaacaa tcttccgatc
    17521 cctgcatgat gatcatggtg ttttgacctt cgaatggatt ctcttgctga ccctcgttgt
    17581 cattggaatt gtcggtggtc tctctgccgc acgtgacgcc ctgatcagcg agctggggga
    17641 tgtcgttggg gccgtcgttc acatcgatca atcgtacact gtctcatccg atgcgacggg
    17701 ttggggaaat gagttctcct attcggatga actgcccgag tgcgctggtg aacagaatcg
    17761 accaacaact ggtcgcccgg accaagggcc cgttggtggg tgttcccagt gactcgctgg
    17821 gcaagggcgt ttccattctg aaataacccg atgttgcgtg ttgcatttgg gaagcaagct
    17881 cgaaggaatg tgtggtaacg aaacgcgtgg tttgcgattc ctcatcctgg agacataaca
    17941 tgggccatcg catcccccga aaaaccggtg caacccgtcg tggaagacgc ggtttaatga
    18001 cgttcgagtg gctgattctg atcacgctgc tagtaattgg cgttatcggt gcctacagcg
    18061 ctgtccgcga cggcatggtc gatgagcttg gagacacagc cacggccatt ctttccgtgg
    18121 accaatcttt tcacacagaa ccgccagagc gtctcccttg tgcggggagc tggggaagtt
    18181 gggaggacga tgacgccgcg gaacagttgg agcgaggccg gaacctgtaa agaatggcat
    18241 gacgcatgcc ttgttgctgg atgagcggag ggtaaggcag gggccaaaac aaacggggta
    18301 acacaaacca caccaaccac caggggcatc agcttttctc aacaacagta tgggactttt
    18361 cgaaatgttt gtggcaatca aggcttttcc gattttggct attgctgttg gtgtcattgc
    18421 tggatgggcc gcgttcaccg acttgcggca acgacggatt cccaatttcg tgaccgtgcc
    18481 agcggccctt ctcgggttac tgtttcacag cttattaccg tctggtgagg gttggtggtt
    18541 ctccctggca ggtttcgccg tgggggcagt gctccttgta cttccttatt tgctcggcgg
    18601 tggaggtatg ggcgacatca aactacttgc cgccctagga agctggctag gacccttttg
    18661 gatcctcgtc gcctttgcca cggggacagt ctgcggtgcc gtcttgaccc tcatttgcct
    18721 ggtgaccggt cgctcactgg aggaattgct cggcattagc cagggcgctc atgatgacgt
    18781 gaaaaagccc actcagccgc gagccatcaa ggccttgcct tttcgcagca agagggcctt
    18841 gccgtttgcg gttccccttg ccatgggaac gtggatcatg cttgcggtgg tgctcatccg
    18901 cgcggggctg tgagtttcgg tatcggttcg ttgttctctg taccataagg aagttgtgga
    18961 tcactgaggc aagacaaacg aatcgccagg agaatcgagt tgtcgcaaaa cgcaaacgga
    19021 gattgaaagg ttatcgatcc taggaacaga tgaagagaca ggtgctttta caccaacgta
    19081 acgcaaaact agttagacca tcgcccatgt ggcattcaat ttgcagaagc taacatcatc
    19141 caggactttt cggcttggaa tgaggagaaa caaacggccc ggacgactga tgaggaagga
    19201 tatgacgagt ttaccaggat gtgatgaggt cctcgtcatc gtactctaac aggtcaaggt
    19261 tgcgttagga aaacacatct ctattcgtgt tgctaattcg atacagagga gacgttcgtc
    19321 aatgaaacgc atcagcccag caggtgtaag tttctttgtg ggggccattc tggtcggcct
    19381 tgtagcggct tacgctgtcc gacatctgtc acaaaccagc cccgagaccg tgactgtgaa
    19441 ccccagaaag acaaagacta tcgtggtggc caagtacaat ttgccccagt atgcaaggat
    19501 caccgaagac tatattgacg aggtccaggt ccctgcagac aaggtcccag aaggtgccct
    19561 gacgctcaag tctcgcgctt tgtatcggtt ggcaaaaacc acaatccttg ccggccagcc
    19621 cattcgcgat gacgacctct atgcggtagg cgaggtgccg cttctctccg agcgacttcc
    19681 tcctggatat cgggcggtaa ccctggcagt ggacgccaaa gcggccgtca atggcatgat
    19741 tcagccggag agtttggtgg atatcacgtt aactgtgaaa aacgacaggc cggagattgg
    19801 cggtctagcc acactgacgt tgcttcgtcg cgtccgtgtg ttggcgacca gccgcaatcg
    19861 attcccagcc agtgaagacc gacctggcga tttgcgcaac atcacagtcg ccgtaacgcc
    19921 tgaacaggcc aataagctca ttttggcgga aaaatatggc acgctcagcg ttgtcctctg
    19981 cagcaaactg gaggaagaga cggatgaagc cacccttgag gacattatgg ctagcaaggg
    20041 agaggtcccg gtcatcccca catcgaaccc tgtaacccgg gccgatagta cgttgtccga
    20101 gccttccaat gacctcgtca acatctattc cctccttgga atttcacccc tgacgcctga
    20161 gccgatgccc gagcaaccgg tcaccaaaac agctcagatc tggcgaggtg ggaacgttga
    20221 ggaggtcacg tttgtggaat ggcaaatccg cgaagcggag aacgcaacct tagtggctga
    20281 aggaaaaacc ccacaaccat ttactccggg aaccttaacg agctcaccca aaaccacaaa
    20341 gacccatgat gcggaggcgg aggactgccc agagtgtgcg gcaaaacggg ccaaggaggc
    20401 agcagctcgt tcagtgggcg agatgccggt cccgacgccc gcatcgcaac tggtaccacg
    20461 aaacgaccag gccaaccatc tcccaggtgg ttacggccga gtggtcactg tccctgtgcg
    20521 tgggcaactc gctaattgac agtccgcgca acaggttact catcgtcacc gtgtttattg
    20581 ttcagataac cggatgaaag agtgatcgga aacaattgtc ccatacaatc tctattcgag
    20641 tcatgggaaa tcaggccatg atgagttttc tctatcgtca aagtgataaa gatggaaatg
    20701 ggcgcaagaa cgtcttgacc gaggctgcat ccccggaaac gtgggaatcc gagtttcaaa
    20761 agctaaaaac gggattgcac aaagagatca tcgagtcgct cgatctgtct cggctggcca
    20821 aaatggatga cgaccagttg cgtcgccacg tccgaaattt gacggaagcc gtccttcgcc
    20881 gtcgttccga tttattaggg aacatcgacg aagagcggct aatcaacgag ctgatggccg
    20941 agtccttcgg cctggggcca ctcgaaccct atatgcaggc ccctgacgtg accgacatcc
    21001 tcgtcaatgg cccgtttgag gtttatgtcg agcgctgtgg gagacttcag gcgacgaaca
    21061 cggtgtttgc tgatgaagag catctcctac agatcattca acgagtcgtc tcccgtgtgg
    21121 ggcgccgcat cgacgaacaa aacccaatgg ttgacgctcg gctgcctgac ggttcccgtg
    21181 tcaacgccgt aattccgccc ctggccctca atgggccggt cctttccatt cgccgttttg
    21241 gccacgagcg cctaaagatc gccgacctta ttgccttcgg ctctatctgc caagagattg
    21301 tggagctact ccgcgccgcc gtggaaggac gtatcaacat cctcattagc ggcggtgctg
    21361 gttcgggtaa gacgacactg ctcaataatc tttcggcgtt tattcctgaa gacgaacgat
    21421 tagtaacgat cgaggattcc gcagagctca agttgcaacg caagcacgtc gtcagcctgg
    21481 agacgcgtat ctctaacgcc gaacagcggg gagaagtaac accccgagat ctcgtacgaa
    21541 acgcccttcg catgcgtccc gatcggatca tcctgggcga ggtccgtggt gccgaagccc
    21601 tcgatatgct tcaggcgatg aatacgggtc acgaagggtc gctgaccaca atccatgcca
    21661 acgacgcacg ggatgccctc tcccgtttgg aagtcatggt cagtatggcg ggtttcgaac
    21721 ttccggtaaa tgttgttcgc cagtacatcg gatcggcaat taccctcgtc gtgcatttgg
    21781 cccggcttaa aggcggcgtg cggcgggtca tgcgcgtcgc ggaaatcatt ggctgtagca
    21841 acggggatta ccaaatgcag gaattgttcg gctttcagca agagggtgtg gatggtgctg
    21901 gccgagccaa aggacacttc tatgccaccg gcaatgttcc gcgatttctc aaacggttgg
    21961 aggaacaggg gatcaagctt cccgaatccc tcttccaaaa acgtgtcttg accccggcga
    22021 ttacatccct cgaataggag gtctcctctc atggcatggg ccgtttggct ttatccgatt
    22081 ctcacattta cggcggtcag tgggctgctt atcctgttcg cgttgatgat ccttgggcgt
    22141 cgatcgaagg attcgaggaa cgcggaggaa ttgcggccag aggacctaga tcttgaggaa
    22201 atcctctata ttccgcccgc aaaagccgct cgaaccgcgt tagaccgatg gttctacgaa
    22261 ttgctcgaag ccgcaggaag ccgcctggat cgttccacgg ctagcctgct accggcagcg
    22321 ggtgctgtga ttggtgcggc aattcccgtg gtgttctttg atagtgtctt ttgggccatc
    22381 gtgggcacgg tgatcggggc ctttgcgcca atcggattct ggaagtttca ggcctttcgg
    22441 cgggtacgtc gaatgagaaa gcaattgccc gtggtgctgc aactgttcgc ggatgccgtt
    22501 cgcagcgggc tctccttgga gcgagcaacc gcgttggcag cacaagagtc aaaaggtccc
    22561 ctacaagcgg agtttgcctg gtgtgccacc cagttggaac tcgggcattc tccagttcgg
    22621 gttatggagc ggatggccca gcgaattccc atccccgaat ttcgtatctt cgctacggct
    22681 gccctcgtcc atcgtcggac gggcggtgac ctggccaact tggccgaccg cttggcacgg
    22741 tccgcccaag atcgcaccga gttctatggg cacctcaaag ccgtcacagc ggggagccgg
    22801 ctctctgtgt tcggtctgac agcaggagtg ttcatcgcgg tggtccttct tggacttact
    22861 cggcccgagt acatccagca atttctcaat catcccagtg gactttccat tttgggcatc
    22921 gctgcgggat tgcaggtcgt cggtctgctg tgggtctggc gcatcctcaa agcgcgttat
    22981 tgaggaccat catgccgtag tgatgttacc gacgaatata ttgaagatta gcgacaaagg
    23041 tcacgcatat gccggatatt ctacttctcg ctttaggtta ctggcaggac ttggctcatc
    23101 tgtggagcga gtggggattg tcgatcctca cgtttgccaa cgtggtctta ctggcgatga
    23161 ttatccgttg gattctcgtc cgccgggccc agcccaaggc gtccgcgggg cttccgccag
    23221 tcgttgatcc cgaaactgat gaccccggcg cctttggggc gttaactcct gcgttggccg
    23281 cgcagattcc ggaatcggcc aaggaaacac gagatttcca gttgctattg cggcaggccg
    23341 gcttgcatcg tccctcggca cgctacacga tttatgcctt tcgttttttg ctcctcgtga
    23401 cacccctcat tatcgcaggc gtttgtgctg tcatcgcccc gcaagcatgg accctgccga
    23461 tccttgtggc cggtggattg agtagcgccg ttctgtcgat catcccaaga atgtacgtct
    23521 ttttgagacg ccgaaagcgc tacgaagaga tccggagcgg tctggccgac acgattgaca
    23581 tgcttgggat gtgtttgacc ggtggcctca gtctcctcga tagtctcgag cacgtggccg
    23641 gacagatggg ggcttatccg gcgctcgccg aagaactacg caccgttaga cggcaggccg
    23701 aggtggggag cttgaagctt gccctggaag acttgacgcg ccgggttgac ctacccgaag
    23761 tccgccaact ttgtgcggtt ctttcccgcg gcacgcaatt gggcagtcaa ttagccagtt
    23821 ctttgagtga gcacgctgac cacctccgcg tggccctgaa gcaagcggcc ctggcacgtg
    23881 ctaacaaggc accggtaaaa ctcgtgctgc caattttgtt ctgtctagct ccggccgctt
    23941 tgattgtctt aacggcgccc gccttcctcg aactaaagca attcttcgat ccctccctca
    24001 tgaaaagcgc cagcggcaag ggcgttagct tcggtactaa ttcggtcgtt gatgcgatcc
    24061 gtgatctcga tcaacgggtg atctttgaac cacccccgac ggaaatcgtg cccccgatct
    24121 ctcaatagtt cggaagtcaa acgagctagt ctacccaata tagggctacg gcccccctag
    24181 gacgtacgac ctcacaaagg ttaagtcgtc gtagaataag tggagaccaa aaaccgccgt
    24241 gtttcgcggc ggttttctct tttcttgcca cctgaggaca cgccgtaagc taaattttga
    24301 gtgaaagagc atccaacaag tacttttcgg ctgagatcga gttgttggga cattcaaaga
    24361 caatccacgg gctgatctcc accggtcgag tggaacgttt agtcttctcg ggaagtaagg
    24421 agggagctgg aggatgtcac agaccccgat gatgcgaccg ttggcttcga cccctcgcac
    24481 cgggatcgaa agccgtcaac tttcctttga tgagctaaaa cagcgaatcc acagcaaact
    24541 tgtcgacaag ctcgatttgt ctaaaattgg gcagttggaa ggagaagtcc ttcgccggga
    24601 aatccgcctc gttgtggagc atttgtgtga cagtgaaaac acaatgctca accgttccga
    24661 acgggaacga ctcatcgacg aggtcttgga tgaaacgttt ggcctcggcc cactggaagc
    24721 ccttctcaaa gacccaacca ttagcgatat cctcatcaat ggccccaaga acgtttatgt
    24781 agaacgtcgg ggcaagctcg aaaaaacaaa cgtctgtttt cgcgataacg aacatttgct
    24841 gcaaatcatt gaccggatta tttccaaagt tgggcgtcgg gtggacgaga cttgcccaat
    24901 ggtcgatgca cggatgcccg acggttcccg tttcaatgcc atcattccgc ccttggccct
    24961 ggacggggct gctgtgtcta ttcggcgttt tgggcgaagt cctctcaaac tggaggacct
    25021 cctcaactta aaggctttca caccagagat ggtcatgctc cttgaggggg ccattaaagc
    25081 ccgactcaat atcatcatcg ccggtggtac tggttccggt aagacaacgc tcctcaacac
    25141 gctttccagc ttcatcccga acaacgaacg tatcatcacg attgaagacg ctgccgaatt
    25201 gcagttgcag caggaacacg tcgttcggct ggaaacacgt cctcctaaca tcgaaggaaa
    25261 aggtgccatt acggcaacgg acttagttcg caacgccctg cgtatgcggc ctgagcgaat
    25321 catcatcggt gagtgtcgtg gcccagaagc cctggatatg ttgcaggcca tgaacaccgg
    25381 ccacgagggt tcgatgacga ccttgcacgc gaactcaccc agggatgcgc tggcccgttt
    25441 ggagacgatg atcatgatgg cgggatttga actgccgctc aaggcgatgc ggactcaaat
    25501 cgccagcgcc gtcaatttgc tcatccaggc taatcgtctc cagggtggcg tgaggcgaat
    25561 tacccatatc accgaagttg ttgggctgga acaggacacg attattaccc aggatatctt
    25621 tcgttttgaa caagacgggg tggatgagaa cggtcgggca tatggacgtt ttgtggccac
    25681 ggggattcgg cccaccttca tgccacggtt ggaagcccgc ggcatcaaat tgccgccatc
    25741 gatcttccgc cagcgcacca tgatggaagc ctaaggcaat tatgacaact cagaaagaaa
    25801 cagtggttct ggatgcctat taacgtaatg gaacgagtga cctcatgacc cagatgacaa
    25861 tcctcatcat cgttgcggcg ttcgtcggcg cggcggcgct cgttgttgcc ctgagccagc
    25921 ttcttaacca aagacatggg aaccggttgg agagccgact ggaggccctc acccaccagg
    25981 tcaagcgcga ctcggagagc aaagagcgga ccccgacggt cctggccgaa cccatcgagg
    26041 ataaaacctc cttgacgacg caactcctca aatactttcc gaatctcgag cggttgctcg
    26101 aagaggccga cgtgggtctt gcgccggggc gtctcgtggc cattgcgacg gtcttggtag
    26161 gtgtgggatt ggccattggc gtagtcctaa agttgcatcc agcgctcatt gcgattttgc
    26221 cgcccgcgtt ggggagcata ccctttttgt ggattctttg gcggaggcgt tgtcggttaa
    26281 aggcatttgc agcccaatta ccagaggcgc ttgatatgtt ggcccgggcg ttgcgttccg
    26341 ggcagagctt ggcctcaggc ttcaaccttg tggccaagga gattcccgcg ccggttggga
    26401 aggagttcgg ccgcgtgttt gaagaacaaa acctcggaat tcctctggag gaatctcttc
    26461 gtgatatggc ggagaggatt cccaacctag acctaaagtt cttcgccacc gctgtgattt
    26521 tgcaacgtca aaccggtggt gacctggccg aaatcctcga ccgaatcagt tcgctcatcc
    26581 gagaacgttt caccatttgg ggacaagtgc aggccctgac aggtgaaggt cgtttgtcgg
    26641 ggatcatcct gatggccctg ccggtcgtgt tgttcttggc gatttaccga cttaaccccg
    26701 attatgtgat gaccctgttt accgatccgc tgggccagaa gatgctcgcg gtcgctatcg
    26761 tgatgcaaat cttgggtgcc attgtgatcc gcaagattgt taccatcaag gtctagagtc
    26821 gctgtcctcc acctgggcga aggtgctcaa caatgaacca agacgtgatt tttgaacttg
    26881 tggtcgctca agggcttgat gccgccgtgt ttttacctta tgccgtcttc ggcatgttcg
    26941 ccgccattgc gtttctggta ctcgaacgat tcagcggaac gacgcaacgc acggtccagc
    27001 gtctggaaga gttcacacga ccacaaaggc gccgcttaac ggtggaggag ctggcggaac
    27061 ggcggcccaa tcggtttgcg gaagtccttg agaaaaccac gccaaccttt gccaagccgt
    27121 tggaacccaa aaacgaggtg gagctaggac gactcaagca acgccttgcc gaggcgggat
    27181 tccgtggaga taaggccgtc cagatcttct tgggactaaa gttcctctcc ctcctagcag
    27241 ggtttgttct gggtggttcg attacacttg ggctttacgg cctttcgcgc aacactacaa
    27301 catcggcgat tattgcggcc ggtttgggat tctacctgcc cgacctcgtt gtcaccttcc
    27361 tagggcgacg ccgtaaggag gcaatcttct tgagcctgcc ggacgccctt gacctgcttg
    27421 tcgtttgcgt ggaggccggt ttggggcttg atcaggccat gcgaaaagtc acggaggaat
    27481 tggccagctc gcatcccgtg atcgccaagg aatttgccct ggcgaatctc caactgcaaa
    27541 tgggtcgccc ccgggcagag gtcctccacg aaatgggagt ccggacggga gttcaggatg
    27601 tgcgggcatt ggcggccgtc atcatccagg ccgaaaagtt tggctcaagc atcgctcagg
    27661 cccttcgtgc tcaaagtcat gcgatgcgaa cccgacgccg tcaattggct gaagaaaggg
    27721 ctcagaaaac ggcggtgaag ctgattttcc ccttggtgct tttcatcttt ccggggattt
    27781 ttgtggtcct cgtcggacct gctgccatca caatgattcg tgaattgttc ccgaagatgg
    27841 ccggcgggta gtatcactct tatcctgaga acgcaaaacc gtctgctcaa aatcggcatt
    27901 cgtggagcgt gtcgcaggcg atggcaaatg ccgccaagtt gacgactctt ccacggatgc
    27961 cttaaatttg ttggttaggc tgctactttg ccagctgtgg attcaatgag cagcgggaat
    28021 gtgcacatga tggagaaagc agaagaaatc tttgagggca aatggtatgc tcaccctcga
    28081 atgcgaaacg ccttaattgc cggactactt accggtgccg catttagtct ggctcatttg
    28141 ggaattattc ccgacttcgc tgagatcgcc atctacgctg tagctatttt attgggcggt
    28201 taccattggt ctcgtgaagg gattgaggaa tttgttacgg aaggagaaat cggcattgaa
    28261 gtcctcatga tagccgccac tgttggctca gctgtgcttg gtatgtggga tgaggctgcc
    28321 tttttggtat tcctctatgg tactgcggaa ggacttgaag aatataccta tgcccggacc
    28381 aggttttcca ttagaaagct tcttgacctg gcgccgaagg aggcacgtgt cctaagagat
    28441 ggagaagaaa tcaccatccc tgctgaggaa ctcaagatag gggatattct tgttgtcaag
    28501 cctggcgagt ccatcgctac ggatggcatt attattgaag gccgttccag cattaatgag
    28561 gcacccgtaa ccggagagtc tatcccggtg gagaaaaaag aggggatgaa ggtctttgcg
    28621 gctaccgtca accaagaggg agcattgaag cttaaggtta ctgccacctt caaggacaac
    28681 accctctcga aaatgattca tctggtggag gaatcacaag agcagaaagg aaaggcacaa
    28741 ctctttattg agaagtttgg aaaaaggtat tctcctcttg tctttctgag tgctttactc
    28801 ctaattgttg tcccctcttt gttgggagca cccgtctcct attgggccac tcgggcggtt
    28861 gttttcctcg ttgcggcggc tccctgtgcc ctggtgatgt ccactccggt cgccattgcc
    28921 gctggcatta caagggcagg ccgcagtgga gtactcatca agggtgggat ttgtctcgag
    28981 aacttgggca aaatcaaagc tgtggctgtt gataagacgg gaaccctcac caaaggggaa
    29041 ccggtcgtga cagatgttgt gccgttaaat ggggaggaat cagaggttct cagtttggct
    29101 tacaatgtgg aaagatattc tgaacatcct ttggccaaag cgatcatcag aaaggcagaa
    29161 gagtcgcgga tcagaagtca agaggttgcg gatttcagcg ccattgcggg atatggggcc
    29221 aaagcaagag ttggcaatag ggtcgtgtat gtgggaaagg aagggttatt cagaaaactc
    29281 ggcctggaaa gccaaaccat cccacaaatc gaaactctca gaagccacgg caagacggtc
    29341 atgctcgtcg gaacggaaac aggcattgac gctatcgttg ccatcaggga tagaatccgg
    29401 tccgaagcaa aagaggcgat agacgaactt catcgcatgg ggattaaggt catcatgcta
    29461 acgggggaca atgaagccac tgcgcgggcc attgcagagg aactggggat agatgaggtc
    29521 agagctgacc tcaagcccga aggcaaggtg gcagccgtga aaatgttgga ggaaaagtac
    29581 ggtacggtgg ctatggtagg agatggcata aacgatgccc cagctttggc acaggcaacg
    29641 gtaggaatag cgatgggaac ggcgggaaca gacgcggcaa ttgaggccgc cgatgtcgcc
    29701 cttatggctg acgatcttac caaagtgtcc tttgccataa atttggggaa gagagcacgg
    29761 agaataagcc tgcagaacat tgttttttcc ctgttgatac tagctatact catccctgcg
    29821 gccctaagcg gagttatgag cgttgctttt gcggttttcg ttcatgaggc ctccgaactt
    29881 ttggcggtgg cgaacggtct ccgtatggca aggggttgaa accggttgtg acaacagctt
    29941 ggcttgctca tgagacatgc cataagctag gagcggccta atgatgtgct atctggattc
    30001 ttgcacattg ctatgtccat tattctgtgg cgactgccat accgtgggga cagttctttc
    30061 ccgaaagtta taattcgttt ttatggcagt ttagccacta cacgtgtgtc ggttcttgga
    30121 ttctagcaga aggagtgatg aatggtttcc cgccgggtag tgattgtggg tggggtggct
    30181 ggaggtgctt cctgtgcagc ccggttgcga cgcttagacg aaaatgccga aatcttcgtc
    30241 ttcgaacgtg gcggttttgt gtcctttgct aattgtgggt tgccgtatta catcggcggg
    30301 gtgattgcgc cgcgggaaag acttattgtc gcctcgcccg agcgattccg tgactatttc
    30361 aacatcgaaa tccgtgttca tcacgaggtg accaagatcg atcgggaaaa tcgtattgtg
    30421 catgtccgaa attgccaaac gggaacatcc ttcacaaggc cgtacgacaa cctcgtgctg
    30481 tcgccggggg ctcgccccat cgttcccaat gtacccggcg tggatttacc tggggtgttc
    30541 accttgcgtc tgcttgagga tgcggagcgg atttatcgct ggctggaaga ccgccaggta
    30601 acgcgtgccg ttgtggtggg cgctgggtat atcgggctgg aaatggtgga aaacctcgtt
    30661 caccgcaata ttgccgtaac ccttgtggaa gctgccaatc agatccttcc cggcttcgat
    30721 ggcgaaatgg tggttccttt ctacgataaa ttagccgccc atgaggtcga aatcctctta
    30781 ggcagcccgc tttgcgcgat tgagccgcac aattctcaat tggcggttgt cgcgggtggg
    30841 aagcgaattc cctgcggtgc ggtgattctt tgtgtgggag ttcgcccaga ggtgacgctg
    30901 gcagagaacg ccggattgga gatcggcgag ctgggtggga ttcgcgttga cgagcaaatg
    30961 cgaacaaccg atccgagcat ttttgctgtg ggtgacgccg ttgaagtgaa agacctggtc
    31021 acaggcaagt ggacatgggt tcccctggcg ggagtggcct cgcgacaggc gcgggtggcc
    31081 gccgacgcca tctgcggtcg cctcagccaa ttccgtggtg ccttggggac tgcgattgtg
    31141 acggcctttg gggtcacaat cgccaagacg ggattgaccg aaaaagcggc ccagcgagat
    31201 ggtgtccgtt atcagaagag ctactcgcat tcaccgcatc acgccacgta ctatccggga
    31261 gccaagtcga tcgcgattaa gttacttttt gatcctcaag atggtcgtat ccttggcgct
    31321 caggccgtcg gggagaccgg tgttgataaa cggattgaca tcatcgcaat ggccattcag
    31381 aaagggggta ccatctttga tttggaggaa gccgaactgt gttatgcccc ccagtttggg
    31441 agtgcccgcg accccatcaa cattgccgga tttgtggctg ggaatgtctg ccggggagac
    31501 gcggacctcg cttattggga agacctggat cacaagcctc aagcggatgc gccacttgtc
    31561 gttgatgtga ggacaccggg agagcacgca gcgggggccc cacctcgggc gatcaatatc
    31621 cctctcggag agcttcgcag tcgcttgcac gaattgccga cggatcgtga attgtgggtg
    31681 cattgcggaa ttggtcaacg ggcgtattat gccgcgagga tcctgaagca aaaaggtttc
    31741 cgtgttaaga acctatcggg aggctacgtg acctaccgca tgtaccgccg cagcaagccc
    31801 gccgattaag aatcgatccc aaaaagtccc ccctctccct ccgggagagg gttggggtga
    31861 aggcaagcga acgtcggaat cgattgagaa aaccggcagc gattcgagcc accctcaccc
    31921 gcgacccctc tcccgccgaa agggtgaggg gagatagtgg gataagctct tggttgcagc
    31981 gtcgcgctac tgaagctggc agaggtcttt tgggccttct agggtggttg ttcacaaaaa
    32041 agctaaggcc tcttgacaac tggaagcatt attagcggct ggcttagaat aaagtgatgg
    32101 ctctgatgtc gttaactggg ttgctctccg gcatacggag gggattatcc ccattgatcg
    32161 gggccagtag gaaatcgacc cgggggctcg agcgaggcgt aatgacaaga ggttccctct
    32221 ttattcctgg ttaagtagga ttgccatatg tcgggcgtat tttccactat cgaggcggct
    32281 attgaagcca ttctcgaagg caaggttatc attgtcgttg acgatgaaaa ccgggagaac
    32341 gagggtgatt ttatttgcgc cgcggaaaag attacaccgg aaatcgtcaa cttcatgatt
    32401 acctacggcc gggggattct ctgcgccgca attttgcccg acgtggccga gagattggat
    32461 ttgcccttga tcgtgaagga gaacacctct ccattgggca ccgccttcac tgtcccggtg
    32521 gatcatcgtt cgactcggac ggggataaca gcgatggaga gggcgacgac gatccgggcc
    32581 ttgatcgatc cgacgagcaa accgggcgat ttttgccgtc ccggccatgt ctatcctctg
    32641 attgccaagg aagggggcgt gctccgtcgg gcgggccaca ccgaggctgc ggtcgatttg
    32701 gcccgactgg cgggactggc acccgccggg gtgctctgcg agattctcaa cgataaagga
    32761 gatcgcgcga ctcggtcgga gttgtacgaa ctggcgaagc gtttcaattt agagatgatt
    32821 accgtcgagg agttgatccg ctatcgccgt cggcgcgaaa agctcgtcta tcgcatcgcc
    32881 gaagcacagt tgccgactcg atatggagcc ggccgaatca tcgcttacgg ggtcaaattc
    32941 gaaaaccaag agccaattgc cgttgtcatt ggtgacccga cacgggtccc ggctccgctt
    33001 gtgcgtctcc attcttcctg ttttaccggc gacttgattg catcacttcg ctgcgattgt
    33061 ggcgatcagc ttcacctggc catggagatg atcggtcgag agggggcggg cgtcctcgtt
    33121 tatttgcctc aggaaggccg cggcattggt ttggtggaga agatcaaggc ctatcacctc
    33181 caagatcagg gaatggatac cgttgaagcg aacttggctt tgggtcacca ggccgattgc
    33241 cgggactatg gaatcggtat ccaaatccta aaagatttgg gcctttccaa agtgcgtctt
    33301 ttgacgaata accccaagaa gacggacgct ttcatctacg gcggattcga ccttgaagtg
    33361 gtggaccaaa ttcccattat tggtcccatc aatgaataca acgcccggta tctagcgacc
    33421 aagcgagaca agatgggcca cctcttgccg gatacctttt gagacgtggt cttttcatca
    33481 cggctaactt ggagaaaagc gggcacctgc tgtttggaac ccgcgagccg ccagcgtaag
    33541 tggtcggcga gtttgtcggc cacgacggtc agtagtcctc catcacttca tctgagcgat
    33601 gcctcgagaa ttcccactca gacggctcgg caatcctcga taactctagc tgtggaggaa
    33661 cgcgcgatgt cgcgcctgtg atcggtttag gcagtggttg ctgaaagctg tcgcgccatc
    33721 tcttaacgcc aagcccccat tgcctttcgt tgccaaagac aatccctggt ggtacgatat
    33781 ggccggttgg aatcgcgagc ttttgagttg cctgtttccc tgacgcggag gaggtgtcac
    33841 gatgtcggcg aaggtgtttc gttatgctgt ttgggggttg gtcacgttag tcgtggcttt
    33901 tgggggattg agtgtcgcgg gctctgccga ggaagggaaa aaaatctaca tcctcaccga
    33961 tcttgaaggg gctagcggtg tgtacaagtt cgcccaaacc cgggagtcag gaccgcttaa
    34021 cgaccaggcc aaagagtact tgatgggcga cattgccgcc gtggtacggg ggctccgcga
    34081 gggaggggcc acggaaatca tcgtcctcga tggtcatgga agccaggcat ttgtgccgca
    34141 tctgatggtt cccggggcca aatacatcac cggcattccg cgaccttccg tcatgcccta
    34201 tctggatgag agctttgcgg ggattgttca gttgggcgct catgccatga tgggcacaca
    34261 ggacggcgta ttgtgccaca cccagtcatc gcggaatgaa aaccgttact ggtataacgg
    34321 cgtcgaatcg ggggagattg cccaggtggc gctgtatgca ggcgccttcg gagtgccgac
    34381 gatcatggtg accggtgacg aagcggcatg tcgggaagcc cgccaattct tcggtcctga
    34441 ggtggttacc gtagttgtca aagtgggttt agctcgcgaa tcggcaatgc tgtttccctt
    34501 tgaagaaacg cgtcaggccc tttatgaggg tgcaaaacgg gctatggctc aaattggcaa
    34561 atgcaagccc tatgtcattg acatgcccat caaagcgaaa aagcagtggc ttgtctttga
    34621 aacgcctgac ggaccgggaa aacttcagac aaaggaaggc gtgatcgaag acgtgcggga
    34681 tatcttccga ttctgatgat gagtgttggc gaaaagaccg cggggcgtca ggcgacggcc
    34741 ttctccttca gggcgacata acgggcacgc ccctgctcgg aaaagatgag tttatcttcc
    34801 cgagccaacc aaccaatggc ctgcataact tcgtctcgcg gccgatccac ttttttgata
    34861 atttgactca aattaagtgg accgttctca ttcaggacgt gccacaccaa accggcggtc
    34921 gctccgatcc tcgccacgta agaagaattc gcttcactca tggctccatc cctttgttca
    34981 aaacattaaa acacgccaga aactcccatc cttgcccgtt tcggaaaaat cacccctcat
    35041 gaccaaccaa catctcaaaa ggtccgactc gcacggtcgg cttcaccgtg tacctatgtt
    35101 cccttgatcg cattttacga acatctgcca ccttttccaa gaaggcactg gagctgcccg
    35161 ccgttcggag gctaattgag ctcgatcttc cacctcggcg cggaactctc aacacactga
    35221 gctttggcca tcttgtaacg aaggcgattt gcaaagatcg gcccattcgt gcaaaagccg
    35281 cttgaaagga cacgacgtcc tttgctctct actcttggca atggtatcgg gcggggtttc
    35341 ttttccagaa gtcggcagta tcatggaaaa gccggacgag cgtaggcaaa gcgaagtgtc
    35401 agcgcatttg tgtaccgtga gagcggggat tttatcaacc cagtaaccta gggaggacga
    35461 cgtgtgaaat cgttctggca gatcgttttg gttgggagcc ttctgacttt tggcgacctc
    35521 cttccgggga aacacgacgt gttcgcccag gaagtcgcta cgacgcccga ggtcccctgg
    35581 acacaagtat ggacccaacc cagtgtggtt gcggaaatcg agaccatcgt gaaggcggtt
    35641 cggccagagg aaatccgccg ccatctcttt tacctggcca aggacccttt gccgttccga
    35701 aaactcaatt acacccggcc cggtcaggaa aagaacaccc tctacgaggc cgacgattat
    35761 ttgacgcggc aactgatgtc gtggggctat gaggtccggc gggaggaggt gcgcgtgcag
    35821 gcgtttcgtc gggaccccaa taagcccaag gcccaccagt attcgccacc ggcgtcagag
    35881 gacccgtggt atctggcgta caacttgtat gcggagaaaa agggccggcg agtgccggaa
    35941 gagatcatcc tgctcttggc ccacaaagac tcgcagagct gggtagattc acccggtgcc
    36001 aatgataatg ccatcggtac ctccggtctt ttggagatcg ctcgcgtgtt ggcccaatat
    36061 cccagcgatc gaacgatacg gcttctttgg tgcaatgagg aacacacgcc gtggacgagc
    36121 gtgacggctg cacaaaaggc caaagaacga ggtgacaaga tcattgccgt gataaaccta
    36181 gacggcatcg gcgctaagtc tgccgaggac acagccgccg ggaaaaagac aaacgtcatt
    36241 ttgtttacct tgccggaagg tgaacctatc gcccagttgg catttgccgc caatgaacgg
    36301 ttcggtattg gactggaaca acggatcgac aaacgacctc gaccgggcga cgatgatggg
    36361 tcattcgtcc gagcaggata tccggcagcc gtggtgatga tcggctctta cccgtatgcc
    36421 gatcccaatt accacacgga ggacgatatc ccagagcgca cggacgtcgt caacgccgcc
    36481 atgacagtcc aggtggccct ggctacggta atcacgctgg atcgaatgcc gtaatccgac
    36541 gaacgtgccg cagaagtagt cggccgggcc aaaaggaacc gtcgtttgcg ataaaaaagc
    36601 aaggccgtcg taacatgcgc cttcgacagt cactcgagag ccgtctgttg gtcaagggcg
    36661 taccgtgtta ctggatatga ggacttctac aacggtgcgc ccaactaaga aaaaacagga
    36721 ccccgaccac taggagaggc ccgccaacga tcggaatcca cccggggtgg cctttcacga
    36781 aataagccca gtaggcatac agtacggcaa gtccggtgaa tacagcacct ccgacccact
    36841 cccaccgcca ggagatggcg acaatccccg gcaagacgag catggggctg acgtgaagaa
    36901 gaaaatcacg aaacccctca agaaaggtct ttccttcacc aaatgcatct agagcaaaga
    36961 cgcttaggaa caggcacacg aagatcccca acactcgggg actccagacc aaaagagaag
    37021 cagatcggtt aatcatgatt gaactttctt gccacggcac tgactcggtg ttaagggcct
    37081 atcccataat atcccctcgc ccgttcggcg ggaaaggggt cgcggggtga gggtggctcg
    37141 aatcgctgcc ggatttctcg ttcgactccg acgatcgctt gccctcaccc tgaccctctc
    37201 ccggagggag agggggaatt ttgggataga ctctaggccc aatagaccaa atcagcccgg
    37261 tggccagatg actgtcgcca aaatcgaaat ctacgattat tatgcccaaa gctgggcgga
    37321 gttcgttccg gctggtacac agcaaaattc cctcgacggc caatctttct cacaatgtat
    37381 tgaaagaatt ttgtccatcg gggccgtcag gcggcgggtg tcaagtcgat cgccacaaag
    37441 tgcttggtac ccgatgatgc aagcatgtct cgtcgcgcgt caaaagagcg ggcgactatc
    37501 gggcacggcg ttacgaaccc gatcgggcga ggctgacgcg gacgtcgctt tgggcctcga
    37561 ggtagcgacc atcgggggcg tagtatcgca caaagacctt cagatcctca tgctcgggaa
    37621 gacgattagg ccagggtaat tccaataaga atcccaacac atcaccgcga cgaacggcgt
    37681 agctcctgac ctcttccggt tgcaaatccc atcgcgcgac acgggctgct ggccctgtca
    37741 tagccgggtc aacaacaacc accgagatcg cagccggttc cagaatgact tcgccgcggg
    37801 agtcgcgggg ctggaccaac acggcaatac cgtcatcacc gtcttcgccg tcatagtcgc
    37861 gccccgtgac gaacttcggc ggaagtataa tccgctcggt catggggcca acctggtctg
    37921 ctttctcgcc tcggctggag ggactcgaaa taggaatcgc ggaggccggg gaagacacac
    37981 ggcttgaatc cagcggcgac gctcggcggg tggtttcttg ggggaggatg tcaggcgtcg
    38041 gctccgcgtc cgaattcgac ggggccagca atagaggacg ggtcacgctg ttttcctcgg
    38101 gtgacggttt ttcctcgggt gatggctctg gtggacgatg tgaagtgctg cctggcaagg
    38161 cacgttccgg ggatgtctcg cggctggggg gcgtcacact gacctcgggt aaagcgaatg
    38221 gttcagttga ggacccaacc gatggcgcgg gggcaagcat gtcgccgcct tcaggccaag
    38281 tgggggcata cagttcccgt gggtgtgaag aaggattagc cttttgggat gttgccccgg
    38341 cccgcgactt gagtcgctcc aactcgctcc ggcatgcgtc gagttcggcc tgtttctgct
    38401 ccagttggct gaccaattcc cagatcttgc tctctaaggc gtaattctcc cgttcaagct
    38461 gagtcacaat ttgaggtgtg cgacacccta tggcgagggc aaccgccgct aggcacaaaa
    38521 ggacaatgaa tttccataat gaggccatgt gaaggtgttt agccgtcacg gtgagggacc
    38581 aagcatttcg atctatcgcg gaatgaccct attacctgga ccgcgaaaat aacgcaagat
    38641 caatctagac attgccgtaa gataggcaaa atatacccca ccaatgtggc ataggttacg
    38701 gcatatctcg ccggtgtctt ttgacgaacg gtgcgctatt tttcccgctt gtcctgacca
    38761 tgctgggcag ttttaataac ctcaacccgc gcgacgagag gcaacaagag ttacccgaca
    38821 ccgcttcgcg gcggggataa atttctcggg cacctgttga cacaataccg caccaagggg
    38881 ttaacgggtt ggcaatagct ccaccgccgc gaatggaaca cggaggggcg ccgctatcgg
    38941 cggtaactcg agcgttttct cgcgtgtacc gacagtgggg gcaccgatgg acattttccg
    39001 cctcgcaggg ggacctgata aacgggtgcc ctctttccgg gattgtcgaa aacccttaaa
    39061 atgcttatga ataacaaccg attccgccgc gacgggcggg aatgacataa agcggaaggt
    39121 ggatggccac cattcgggtt gtctgccctc ttgccaaaaa accgttatgt tcccgctgta
    39181 ctcggccgag tgccagcgga aatagagaat gcgcctctag ctcaattgga tagagcatcg
    39241 gtctacggaa ccgaaggctg caggttcgag tcctgcgagg cgcgtttttt tgtttcttgg
    39301 tccggtcttg ccggtcgaag cctcccaaag gttaatctgt cgaacgagaa aggcgcaaat
    39361 tgtggaggtt gcgcgagata tttgagctgg ggcaattctt cctgacttgc tttgctcggc
    39421 ttgtgaaaat tttgctgttg attttcacgt ctgacggtct gagcctccgc accgcagaaa
    39481 agaacgtcta aaggacagtc tgccaggcca attcgcgtgc taatttgccg ttccagaaca
    39541 gcgccagacc catttattcc actcgtgaat gggcatttat ttgcctgcca agccggcgcg
    39601 accgcctgat ctcatccaca taactacgac ccacgcctgc tgggcagcga aggggcgatc
    39661 catcgtggga gtcgcagcgt tggaggacta aatcgcagcg gacatgatcg aggacgcttg
    39721 cgaggccgaa aagtcctctt ttcctaaggc acacaatttg ctaaactccg ccgctcatgc
    39781 cgaagttgtc ggctaatagt gcaatctggg cgtcattcaa tccggcagga cgcttggaaa
    39841 gagttcacca taaggagcca aggatggttc gttgcacagc acttgcactt tccctcccgc
    39901 agcagctttc tctgagactt ctggcgatat ggtcgatcct gttatgtgcg tcgatgggca
    39961 tggtatcacc gcacatcctg gccgggacgt ttcccaatct gttcaccttg aaccgtgtcg
    40021 aggctgatcc gaacaaggat tactggctat cagaagaaaa tggcccatgg atgatcatgg
    40081 cgtgtagttt caccggtgat caggcggaac aacaagcccg agaattggtc ttggaattga
    40141 gaaaacgcta caagcttcca gcctacatgt acgaaaaaac gtttgacctc aaagctgaag
    40201 gcggatctaa cccccgtcgc tatcggtatc aacagggcga gagaatccgc gaggtcgccg
    40261 tcctcgttgg agactttccc tcggtcgatg atccccgtgc ccaggaaacc ctacaaaaga
    40321 ttaaatgcta tcggccggcg gcgctcgaat cgcgtcctga gaaatccacc tccctaacac
    40381 tggccgcgct gcgctcaatc cagcagtcgc tgcttacgga tgataacgaa aagaagcaaa
    40441 agggcccgat gcggcacgct tttttgacca caaatcccct tttgccggac gagtattttg
    40501 ccccgaaagg cgtggacaag tttatcctgg ccctcaatga aggttacgaa tacaacctgc
    40561 tgaactgtcc tggccggtat tcagtgcagg ttgcccactt tacgggagaa gtcataatcg
    40621 atcagcgaga gatccaggcc atcgagcgcg gcgaaaagac attccgcagc tccctcgtcg
    40681 atgctgcccg caaggcacat gaactaacac tcgccctccg tcagaagggt tacgaggcgt
    40741 acgaattcca cgatcgcgga gccagtatcg tcaccgtggg gagtttcgac tcgttgggaa
    40801 ctcggcggcc tgatggcaag attgaacttg accctcggat tgtccaaatc atcaatacgt
    40861 ttgccgcgga acctgcccca ccggttccgg gggtccccac gctcggttac aagcctaaga
    40921 cactgattgg cattccgttt gacattcaac ccacgccggt cgaggtccct aaacggtcat
    40981 ttgctaccga ctacgtccgg cgttgagcat tttcgaccct cgttgcggat gcaagcgtca
    41041 ttcaggtcga tccgaaatca cggcgacgcc ttcgttttcc aagtctagca gtgactggat
    41101 ggatggcaac aaggattcag gtaaaccgtt ttccgtcggc gcgaatgttg tttgaatcgt
    41161 gccgaagtcg ggttcacctt tccggaaatc agcaaccgcc gggctagcct tattgccgac
    41221 cccaacctgc cccaccgtga cacttcctcc ggaggctgcg ggtgggaaaa ctctttcctc
    41281 gaaaccgtcc ggcgatttct ggtacgacgc caaacgatcc ttgaggcgtt gcagcgctgg
    41341 ggagagatgc ggttgagaac cgctgttacg actctccccc accgggacgc cttgagaagc
    41401 tccagtgaga ccgctaggta aaaccggaga aatggccgta ccattctcgt ccgaatcccg
    41461 gttcttaaac tcctcgcctg atgcgaagcg accctgatta agactatccg ttggattggc
    41521 tgtgatcgtt gggagaggac gcacctccgt ttgaggtgcc ggcgccggaa ccgcctcggc
    41581 gcccggtccc ctgttttggg aacgaatcgg accggggtcg atccaatggc cgcgatcgtc
    41641 gagttgcatg gtccccatct ggaagtcgag gaccatccgc tgcacttcat attccaccca
    41701 agccccgaga aaggcattct gggcactgag aagtccggaa agagccgtca ccaggtcgcg
    41761 ggccgtgttt acaccgaatt gtgaggtctc ccccggtcgc ggcggagcac gcaagcgctc
    41821 tcgggcaagg tccacttggg caatggagac aagcactgcg gaacggcgca gctcaaacgt
    41881 gttttgtccc aaccggatat tccgtagggt ctcccgcaag ctctgggcaa tctggtcctc
    41941 aaaggcgatg taatctcgcc gtgcccgctg aaatgcgatg agggccgatc gatattgatt
    42001 cctttcgacc agacgagtga acggtgcatc aaaacggaaa ccgattctca attgtcccgt
    42061 gttgccatca aagctaaacg ggttgtcggt tgtcgtgccc aagtctccac taaaggtcac
    42121 atccaaatca ctgcggagcc gattggcggc aatctggatt tgacgccatt ggtcaaccaa
    42181 agctgcacgg gcattcatcc aatcgaggcg gttttccctc gcaatttcta aggcttcttc
    42241 cggtgtgata tcaaccggag tcagcgtcac agaatccaac cgcgcccggg cctgaatcag
    42301 ggagaggtcc gacaactgaa gtaaaagtgg ctcaagcaat tgcatcgcca cttgctcctt
    42361 gggcgacgcc ttaagttccg tgagagttcg agtgatggaa tcgatacggg agcgaagaat
    42421 ctccgactgg gctcgaacct cagaatattt cgcttgcaac gcaaggactt cctcctgggg
    42481 cgctgttgcc atcgtttgcg agagcttgtt cagttcctcg tccgctacct tggcctggtt
    42541 ctgtaactcg gccagttggg cctctagttc gctgaccctc ttgtttgtca cggaatcggg
    42601 gtcttggatg aattgctgta aattctccaa cagttgtttg agtggttgcc gagggactcg
    42661 ctcttcccca cgtgcggccg gcagtttccc ttcgagaaga agaccaaagt cggcgatgat
    42721 ggcctgatag cgggcgtcca agtctgcgat agccttgtct gcgatcgtta gcccttccaa
    42781 ctgtccgtcc tccgttacca gtttcgagta aaactcgcgg aggagggttt tccgcttggg
    42841 aaggacggcc gtgagacggg caatgtcgtt ttcgacgatc tccacctggc gtgccgtttg
    42901 ttcgtagatc gaccgcatct gatcgagcca atccggcggg tttggggtgg cagggtcacg
    42961 caacgcccgg actagcaaag tagtccgcct ttgcaggttg agtagtcgtg gatcaacaaa
    43021 gtcgaactga gttagaaaat cgtctgagat ttccacgggc aattgcggcg gaagacctaa
    43081 actgaccttg aagttgtcga gctgagactg ataggacgac cgggcggaca ataattggct
    43141 ttgggcgtta taaagggact gcagggccag gtccacttga tatcggtcga tgcggtcggc
    43201 ctgatgggcc gcaagcagtt gctcgtagct atcttccaat ccttgaacgt tctgttcctg
    43261 gttccggatt tgaacttgtg tttgcagcag ccctaagaca ccaccccgcc caggagtgac
    43321 tgacggggtg agcgagccga tgctcaggcc tcccgcgctt ggaccagcgc caggatttgt
    43381 tcccgtgacg acttgaagat aaaaccccct ctgataatgt gccatctgac gaatgttggc
    43441 caggaggttg cgctccgcct gcgtcaggct ttctaggata acggccctcc cgcccgcacg
    43501 gagcaatggt tgaaccagcg aaaagttaag taatgaagcc gaagcgtagt tgtcgcttcc
    43561 ggagaattgc caaacgaggg aattggcgaa accgacgagt agctcggtcc ccgtcgcacc
    43621 cagttttctt gcctgagcat ctgtgtcaag ttccaggagg cttcgtggtc ccccggcacg
    43681 cttgctaccg ttactcgtga aaaaggtcga atgccccccg aagaattgaa catcaaaccg
    43741 aaaacgctcg gccgtcacat tcaaagccga cagatagaga ttttcgattt gacgctggta
    43801 ttccctggaa tggacgagtg ccaaatcgac ggcgcgggcg cggtcaagca caacaacgcc
    43861 ccgctcgtcc cggggaagat aagcccgcca gaacggactt tcaacatagt aggtgtcacc
    43921 gttgtcgtgc caatgcggcc aacccttttt gccatccacg taatgcatca attggtgcga
    43981 aaccggatcg tcgggaggca tgggctcggc atcagggcag aaggggtcgt agtaccggga
    44041 ttcctttttg ggatagaccg ttgtatcgcg gagtcgccac cgtgggtcgc gctgcccctg
    44101 ctggagaagg gtactcactt ccatgtcggc ctggcggcgg taggctgtgc gatggcagcc
    44161 gcccagacat agtgcggtta ggacaacggc gccaagaacc acctcgtacc acatgtcggg
    44221 aagcacttta gcgcagaggg cttggatcag tctgccattg aaaccccacc ttgtcgctgt
    44281 tctttgggtc gatggtcgca ttctttgacc tcgttgacac aaacccggaa acggcctcaa
    44341 agcaagaagg tcacccatta atcgttcggc ccataaaatg cgtaaactta agcaattgtc
    44401 tgagatttgc cgaaagtaac ggctttgccg gccagatacc tctcaagtgg ggggtagcgc
    44461 gaaaacgatt gcgaagaaat ggttaaagct tgacaggggc aggggggcta aataccatcg
    44521 aacgtaacgg atgggattgt ggagaaagta tccccggcag gtgcgaagtt gtggatgagt
    44581 gcccggggaa acggcctggg gtgtgggctt ggtttgttaa aaaatcatga gcgatcgcgg
    44641 ttaggaggtt cggtaggctg gagagggtgt accagggacg ggtaacggct ttgggacatc
    44701 tggaacttct taggaatctc cgttcgctgg tggaaccaca atgagcattt ggaataagat
    44761 tctcatctgc ctgattttgc tcgcgtccct ggcgatgtgg tacttgggga tgcgcgcctt
    44821 ggctacccac cgtgcctggc gaaccgccgc caaacgattg caatcagagc ttgcccaact
    44881 cgaagcggaa cgtgaacgat tgattcacgg cgatggcact acgaagggaa ttcgccagct
    44941 ccaggtggag ttggccaagt acacatcgca gcgtggccgc gtctggaacg gttgccggcc
    45001 ggttcgggtg gacgttcggc aggatcccca agcagggctc gtggttcaag tgagtgtgat
    45061 cgtcgaccga ctcgggctag ctggtttgca ggaggttcaa gagttgcccg tcgattcgct
    45121 cgtctatctt ttcgacgagc gtgatattca acaaggcggg gtctatctgg gggcgttccg
    45181 cgtggctgcg gtgaccccgc ggcagcaaga tcagcccgct atggtccagc tagaatctgt
    45241 tctccatctg accgatcgtg agttaagacg catccaatct gccatgcagg cagcccaaca
    45301 aggaggaacg ggctggacca tttgtgaact gctgcccaag gatgatcacg agattttcgc
    45361 cgggttaaat ccggaagcgc ttgcgcagat cctacccaag aaggtcgtgg atgagtacat
    45421 tcgagatggc aaaccaagtg atcctaataa ccccaactca ccgccgtttt tccgaaagct
    45481 ccgagattat gagtcggcca ttcgctacct tcatttgaaa cggagcgaac tcatggatca
    45541 ggttgctgcc cgcacggcag acaagggtcg actggaggca gccgttgctg actcgcagcg
    45601 cgaagccaag gcccgcgatc aggaagtggc gcaattgcaa gaagtgctga agcgggccga
    45661 gttcgaacgg gatttggcgg taaatcatct aaaggcggtt gaagacacac tcgctcgtgt
    45721 gaccgtcgct cgcgatgccc tcatccagca aaatcgggtc acagcggcgg agatcgccca
    45781 agcgcaagag aaggcccgac aacttatcga acagcgtgtc cgcagcgtgg cacaaagtga
    45841 acagccggcc tcgtccgtaa cggcagaggg aacgcggcgc taacagcgag tcgccggctc
    45901 ctcctggaaa ccttatcgcc cgtcggcatt cgtcctgaag taaccgcgaa ttttatcggt
    45961 ccacactgaa acgaatgagg aggatgtcgt cgtcttggac gacataatcg cggtgctcgt
    46021 gacgcaccag gttctgcgcc ttcatttccc tttcacttcc caatcgaatt aagtcctggc
    46081 aggacattac ctcggcacga atgaaaccct tagcgagatc ggtgtgaatg gcctcggcgg
    46141 cctccacggc cgttccaccc ttgcggagga gccacgcgcg gacttctttt ggccccgctg
    46201 tgagaaacac atgtaacccg gccacatcca tgatggtccg gatgagggcc ccccgatcgg
    46261 cccctcccac cccgagttct gcctggaatt gttcccgttc ctcgggcgac atcttggata
    46321 gttccagctc caaggccaaa ggcgcggcaa aaaagggcac atactcggga aacatcccac
    46381 gaaaacgtga gaaatcgcgc tcatcatcgg ccgtgttgac cacgaccatc tgtggttttt
    46441 cacaaagaag ccgaaaggcc ctcgtgacac gttgttgttc gggagtcatt tcagattccc
    46501 gaatcggatt gttttcctcc agtcgctgtt taacccactg aagagtcttg agttcctctt
    46561 ccaggtgctc gcgttccgct ttcggatggg gctttttcag nnnnnnnnnn nnnnnnnnnn
    46621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46681 nnnnnnnnnn nnnnnnnnnn tcgggaatcg tggccatggc ggattgcccg gaaaacgcct
    46741 gtgccgggtc cggtgggacg cctgtcagcc actcgaaaag cgtactcttt ccgcatcctt
    46801 ggtaacctac aagtccgatc ttcattagtt aagactccca aacggccaca ccgagcgtgc
    46861 caccagctat gttcaagcga tctttgcact ggtcgttaac aacgatgcgc gcggcaggtg
    46921 gacggcgatg gatcgcaacg caagatcgag caaatcgccc cgaaagggtt cgtcacttcc
    46981 ctctattatg gcacttcacc aggcggtggt aagagaggca ttgggcgaag aggctctctc
    47041 gtggatacat cattggctcc tggcccctgc ggaggtgggc catcgcctcg atctcgatca
    47101 gatgagccgc cggggcctgg cggcggttct cgttctcccg gtcccggtcg cggtccgcgg
    47161 cgctcgccac cgtgtccgcg ccacggtcct agggtgtcaa agcccgggta gcgccatcgc
    47221 agatagcggc gggtcagttc gcgctgcatc tcgtcaggtg gcagcgctaa gagcttctct
    47281 cgttcctcgc gcgtcaaatt ctcctcgaca aaccggccca actcctgttc actgaccgcc
    47341 cgtcgcatat ccgcccacac ccgcggcatg acgtacttga agaaaacgta gctgcgaatc
    47401 cattgggcga cacgcttcaa ttgttcttcc gtggaaagag attcaagccg agcccgtgtg
    47461 ccaggcgaaa attgcttccg taactcctct aattcttgtg gtgtcaaagc cgaggcctgc
    47521 tcggggtgat ccagttgcca ttggaaccac gtgatggcca aaaagtctct tcgctgttgc
    47581 gggtccgtca cacgactgag tctccgcagt aagtcctgtt tttctccgct gttcaaatgg
    47641 gctagcaatt cttctccccg ctccgcggcc aagctctcca caaaacgcac caggacagcg
    47701 acgtctcccg gcggcaaacg ctcctctggt ggaaccctca tcgagtcttg gagaagcgtt
    47761 ctcaatcgct cgggtggaaa attgaataag gcccgactga acgccgcggt caacatctcg
    47821 ttgcgggtct tctgaatcca gcttattcgt tgcgccggtt ccagagtctc taactcgcct
    47881 cgctggctag gtgtcaaaag cttgaaccac tcgtagtacc gctgcataac cgcaatcaat
    47941 tcgtcgcgat ctggagcctg catcagctcc tcgtgaatgg ctttcatacg tgcttgttca
    48001 cttggcgaca actgctggaa tctttcaatt ttccgacgca attcgacctt ttccgcatcc
    48061 gtgagtcccg cgatgtgttt gcgaatctcg gcggggtccg acaacgtgat aacagaggat
    48121 gacgcgggat aggaaacagg ctttgacgtc accacggccg tcgaaccgga aggtagcttc
    48181 tctcccgaac tctcagataa cggttgtcga ggaacctggg acggtcgatt atcccgaggc
    48241 tcgggtctca gatttgaagt cggtccccct tcgccgggta tttctccggc accagccggt
    48301 cccctttggc cttgcccggg gccgggcgga ccaggcggtc ggaacatccg ccattcatca
    48361 acctcttggg cgtcgtccgg attgaggtac tcttcttcgt ccgtatatag gccgctgtcg
    48421 cgaagcattc gcaggaaggc cacattttca atatggcggt aataatcctg gttttgtatg
    48481 accgcataat gctcgattag ccatttgttg gggttttccc agaggaaaga cgccaattgc
    48541 cagccacccc aaacggccaa taatgtaccc aagaccgcca cacctaaaac aattgcccgg
    48601 cgtttccatt tagtctgcgt ttgagccacg acttcggaag ccgcagactg agccaccatc
    48661 tcgatcgtgg ttcgggtaaa atcttcatcc acggtcgggc ggtccagcac atccagccaa
    48721 tcccaagtct cggccaaacg ctgaagttct gaacgaatcc gggtatcctg ttccacgagc
    48781 cgttcgattc gggcagcctc ctcggatgat aattcaccat cgaggtacgc gacgagatcc
    48841 tcgcggatgg cgtcaatcga aatatcggaa cgtttttgaa atgccgaatc gctcatcgcc
    48901 cgtaactcgc catattgaca tgatcatccg cctctcgacc atcgacgtgg tgacgtggac
    48961 ctatgttgaa aacgtccaca atttttttgg cctcaacctg atcttatccg gctccgccca
    49021 agctaatttg agaatcactt tgcgagtctt caatcatggt gctccgtttc gagtcaacgt
    49081 cgtcggtgcc ccccaatcca atgggcattt gaccgctttg gaagtaaggt tctaggatct
    49141 cccgcaaacg tgtccgcgct cgcgaaagga gcgactttac tgccttgggt gacattttca
    49201 tggcggcagc gatgtctgcg taactcatgc cctcgaactt acagagcaac acggccaacc
    49261 gctgccgttc attcagcccc tccatcgcca tgcggacgat ttctcgcgct tccaatttat
    49321 ccagccggcg ggccgggatt tgaccactgg aaacctggat ggcatcgtca agggcgtcaa
    49381 aatgctgaga atcgcctggc tgaaacggaa ccgtttgttc tcgccttcgg caccgacttc
    49441 gcagtgcatt catggccaca ttgttgacaa tcctaaaaag ccaagtggca aacttggcat
    49501 tgggctcata gctgtggcgc gatcgataga ccctcagaaa aacttcctgg gtaaggtcct
    49561 cggcctgatg tcgatttccc aacaagtttt ccagcactgc caggacccga ctctgatagc
    49621 gcaacaacaa ttcctcaaac gccgccgcgt tgccgttgcg gacctctaac atcaatcgca
    49681 catcagggtc acgaagtgcg gccctttggg tggacgattt gctttccgtc aagcgctatc
    49741 ctcgtgtgga atcgagccgg tgagtattag cctctgtcca tcatacattc tggacatctt
    49801 tggagtacta tcaactctct atgatggcat ctcaccggac aagattccac atccgaccac
    49861 cggagcgacc cactaacgtg ccgatcttcg ggccaaggtt aatttttccc gcgtgcagcg
    49921 actaaaaaca cggatacagg ttttccacga atcgtagtac cagcccgctg aactaccacg
    49981 attccctctc ttgccccagc tgccagagcc actgctcgcg gtgggacctt gcggctaatc
    50041 tttccaccct cgtcactcta tgagttttaa ccccaaaagt gtcgttgggt tctgagcgta
    50101 ccttaccccc aatgaaaggg ggatgtctgc gtccaagaca gcgccgaatt gttgcctttt
    50161 acctcgacag gaatcaaagt gaaaagaagg ggcaaaatcg gcatattcgc tattttcgtt
    50221 gccgtcgctc gaggtggctt taaaagggtg gagccgtgac gctatgacat tgcccgtgtt
    50281 accaagccgc ttcagggatg cgtcaaaccg ggatttcggc ggggcgtcgt aggtaaatga
    50341 gggaacacgc catgcccagc gatccggttg agctgtcgct ttggtgtcgc ggcacactgc
    50401 gtgtcagtgt tgagggtctg gggcaacgag accgcgtcac cactacccaa ccgttcgccc
    50461 ggtgtggaag acttgacact tgcgatgtgc gactgacggc tcccggtata ccgcgacggc
    50521 ttctctacct tcacgcaaca ccccaagggc tgtttgccct tcccctggtg gaactgggcc
    50581 ggttcgctcc actgccatcg ggatggttag tccccggagt gccagcccgt gtgggaccat
    50641 atcgggttaa ttgggaatgg gagtgtcagc cgcccagtgg cgcggccctt gtctctgatc
    50701 ctttcaaatt ggggagtctc gtcgagaagg gttcggccaa acaacgctgg gaattggata
    50761 tttatgtgga tgggcatcgg gtcggaaggt acgaattaag ccgcgtcctt accctcgttg
    50821 ggcgtcatcc ccccagtcac ctcatgatga catcgcggag tgtgtccggc tgccattgtg
    50881 taatcgttgc cgaagaaaac cagctctggg tggttgatct atgcagtgcc aacgggattc
    50941 agctcgatgg acgggcagct cttgcgggat ggtggccggc aggtgtaccc ctccaattgg
    51001 ggcgagttac acttgtggca aagccgatgc cttcgcccgt accaatagga acagccttgg
    51061 ccaaggccgg aacttcagcg aagtctttcc aacaaccatc gtcgcaagtc acgagcgagc
    51121 cgacgggtcc agtgcgaccg gcggatgttg gaacccgtag cgaaccaggg cggtcggcct
    51181 cgcatccgct tcctcctgac gctggatcgc tccaggaaat accccgtgcg gataattcga
    51241 caggcgagac actccccact gttttcaccc cggcaggtgg tgtaaaaaag ggggcattcg
    51301 atttggaggg gccagactcg atggatcatc ccatcgagaa gggtcctggt gaaccctcgg
    51361 cgtcggcgga gccggtcatc ttgacgggtt cgggcgaaac aacgggtctt ccagaagtga
    51421 taccggattc gcagaggcct tcgggagcca caccgtttga caatctcgtc gtacatcaag
    51481 agttcgtaga cgcagagtat ccccctgtgt ttgtcgatac ggaaatcact gtcgaggaat
    51541 gtatgccgga aaaccgccta acggcgcaag ccaaggacta tgtgctttgt cttttcgctg
    51601 atgaatccgt cgatgatcat tgcttatcag ttagcagcga gaagtctgaa ctgcctcatg
    51661 aatcaacgga ccgtttggaa ccgcacgtcg cgggtgacgg ttttccgcgg aaagatgcgc
    51721 ctcacggcca gttgacggac gccgccgcga ttgagcaaaa gacgcaagaa cttcttctca
    51781 aagaacagct actgatagcc tgggctaaga cactcgatca gatgtccgag gccatcctgc
    51841 atctgcaaga ggacctggtt gccgaggaag atcgctttgc cgagcaacaa cagttgctgg
    51901 cagaacagga aaagcagttg atcgtccgac aacgcgagat tgatgcgcgg gccgagcaac
    51961 ttgccatcct tgaacaaact ctcttgcaga gagagcgtga gctcgaagcc agagagaaag
    52021 cacttaagga agaccgcgaa gcgctggtcc gcgatacgca caaccttgct gaagaaagac
    52081 aaacgattga gcgattgcgg aaagagcagc aggggcgtgc agaagaactg gtggccttgc
    52141 acacgtcgct ggctcagcgt gagcaagaag ttcgagaaaa acagcaattg ctggcggagc
    52201 acgaggcatc tctcaagcaa aaagccgaac aactatctga agaagctcga agactggcag
    52261 ccctggagca cgaattgaaa tgtcgcacgg atgccttgaa cgccaaggaa caagacctat
    52321 tccgtcgaga gagcgagcag cgtgaggcgg caattcgttt tgccgatctt gagctgcgtc
    52381 taagccagca gcaacagatg ttgcttcatc ttgagcagga acttgagagc cgcaaggatg
    52441 agctgacgcg tctggaacaa gggctccttc ggcaacagaa agaactctcg ataaggcagc
    52501 aggccctcag cgaacaggaa caactccttt cttctcgatg ccaagagttg gcaaatctcg
    52561 ccagccacct tgcggaaaaa gagcgtgaga tcgccaccca agcgagcaac ctcgcgagat
    52621 gggagcagaa tcttcaagag tgggaacgag acgttaaaga tcgggcgtcg cgtctgcctg
    52681 tcgtggcacc gacagaaggt gccgcggagt ccgaaattgc tactcaaaca acaggcaacg
    52741 aacaagaatt ggccacaccg cagcgggttg agtcagaggt cgttgaggcg caaccggcgg
    52801 aaggcatatc tggggcagaa gggtttagct cggtcactgc tgagtcgagc gtggccgaac
    52861 taccgggggc tgtgttagcc acttcgatag ctccacgggt cgagatcgac aggctccgcg
    52921 aggaagattg gcctgttatc ccgctggcca agattcatcg tcgttggcgg gcacctgcgc
    52981 caattgcgat aggactcgtc atcgccgggc tggtcgcctg ttctgtgtgg tggttttgtc
    53041 ctcgggaatt tatcacggct tgccgcatcg agttccccga agactcgccc ttgctttatg
    53101 tgcgagatgg aacgcgtcct cggggggtag tggaggagtt tttcccttcg atgcctgcag
    53161 ttgccgccaa cgatccccgg gttgtgacaa gacttgggca gattcccgag attcccactt
    53221 tgttaacaaa tggcgaaaag acctgggatc aggtcctggc gtcggcgaaa gtcagccgat
    53281 ccgagggcct tcccttcaca accatggtca tcacgacaag ggatccaacc aaaactcagc
    53341 aagcgataga acgatggggg aacgtctatc tcgccatgtt ggctgagtcg ttccaggaaa
    53401 aggtttcgcc cgctctcgcc aaggccaaac agcgatggca aatcacgtct cagcaattaa
    53461 aagagcacca ggcgagtatc aatgatttga agcagcgata tcaggtcgaa catcctcggc
    53521 aactggaact gcaagctcat gaatcgttgc acatggcaga agtgactcaa aaagagattg
    53581 accagctcca ggctgaaatc acggcacaga agcaacgcct agagcaagtc aaggcagttg
    53641 ctgatcggcc tgaacaagcg atttctgaag aggaagtcat ggccgccgtg gcagaacgcc
    53701 tggcgcaaga cgggctgacg gtcgaaactt cagatcccgg tgcggacgcc gtgaacccag
    53761 atcccggtat agcggatgaa gctgaggcaa acagtcaaca gcctcccgat gggtcaaatg
    53821 caccgagatt gaatgccccc gaccctggca gccgttacga aatcgtacga caacttgtgc
    53881 gaaaagagtt atcggagcgc aagcgccagg atgcggttct aactgtccag gttcttaccg
    53941 ccgagttgga ccgtttgcag gcccgattgc aaaaggccat ggagcaaaaa gcccaatacg
    54001 aggcgacagc cggcgctttg ttagaggcgg caaagcagtg gaacaacctt gaagtggaaa
    54061 ccgcaaaatt agagaacgag caccaacaac tgacaaccca aattgatatc ttggaaaagg
    54121 cactcgctca gagtagaata gcgccgcact tggagatttg gtcgtcccag caacggccct
    54181 ggatgccttt tgcggcggcg gcgcttgccg gaggcggagc gatgatcccc tttggcctgt
    54241 gggggttgct ccgatcgctc aggaccgtga gggtatttcc ccccgcggca attgggtacc
    54301 agtctaagta tccacacccg acaccggatt agacaatcgg agaaaggacc aggttgagtg
    54361 ccccatcggg aggaagttcg cttgggaggt tcggcagaac gcagtgtgaa ccaagtagtt
    54421 aacactatcg caccttggtg tgtgagccag aaactatatc acccgttttt ctcgcaggag
    54481 atcgcaaaat atgttcggaa aggtctcacg gcggcgtgtt ttggcagggt tgacttcagt
    54541 ggttgcggtt ccaacgttgg ttcgttcgtc cgcacttggg ttagccggct tggcacccgc
    54601 cagcgaacgg gtcacgttgg gtttgattgg ctgtggtgga catggcatcg gctggaatct
    54661 tgcgatggtc tttcgccata atgatgccca agtcattgcc gtgtgtgatg tggatcaacg
    54721 acacctcgcc gctggaaaac aacgcgtcga tgagcactac cgtaacgtcg ttggggagca
    54781 atatcgagaa tgcgccgcct atcgagattt tcgtgacctg atcaatcggc gggacattgt
    54841 ggccatcatg aacgtcacac cggaccattg gcatgtcctc ccggcgttga tggcctctga
    54901 atgcggcaaa gacgtcctct gcgagaagcc cttgacgctc tttgtcgaag agggaaaagt
    54961 cctctgccaa accgtggcca agaataagac ggtctttcag acagcttccg aaaatcgctc
    55021 gattgacgtc tatatccagc ttgttagctt agtccgggcg ggtgtgatcg gtaagctcaa
    55081 acacattgaa gtccgcctgc cgatgggaaa tacgaacacg cgggtggtgg gagaggccaa
    55141 ggatatgttt tatcagagct ccccgcagga agttcccgcg tatttggact acgatatgtg
    55201 gctgggacag gccccctgga tgccttacat cccggcacga cttcatggca atttccgctg
    55261 gaatctcgcg ttctcagggg gtgtcctcac cgactggggt gcccacatga tcgacttggc
    55321 ccaatggggc cataacactg agcgaacggg gccggtggag gtagaaggcc gcggcgactt
    55381 tccgccccgg gacgcagtct tcaataccgc gcctacgttc gatttactct atcgatacag
    55441 tgacggcgtg acgatgcggg tgtccgcggg acaaggcgac ctcgatcccc agcaggtgaa
    55501 acccgacacg ccccttgttg ggcggactcc ttctccgggg atccgctttg aagggaccga
    55561 cggatggatt gaatctcata actggcgcgg agtgcttcgg gcgagccgca gcgagatgct
    55621 caaggtggaa gtcgatccca aggccctcgg aatctacatt ccgggagagg ttgtcgcccg
    55681 agactccgga cggggtggcg agcaccgcaa ctttctcgat tgcatcaagt cgcggcagcc
    55741 gtgttacgcc ccggccgagg ttggccatcg caccatcacc atagcccaca tcggcaacat
    55801 cgccatgttg ctagggcgaa agcttcgctg gaatcccgat aaggaagaat ttttgggtga
    55861 cgacgaggcc aacgcgatgc tcacccgcaa acagcgagaa ccttggacga tggccaatgt
    55921 gacatcctgg atcaagtcag gtaactccta gggcgtggct ttagatccgg gaagcctgcc
    55981 cccgtccact ctacgcaact atcccacact tgggcggggg tattttgtcc ctctacatgg
    56041 caaaattgct ctggacgggg tgaaccggtc tgaggtacat taccggcgcc gtaagtctgc
    56101 ggagaggaac cgcacgtggc tttattgact gtggccacga attcatggag gtaatttggc
    56161 atgcgagctt tctcttgggc cttctcgcta ctcgttttgt tggagtttgg atgtctggtc
    56221 acggatggcc actccggatt aactgcgcag gaggttcctc caacaacgcc ttcgttatcg
    56281 acgacagcgt cgcaaccggc gtcgcctgag gctagtcgtc ttcaagtctc catcggccag
    56341 gcagagaaca gtcccgccgg cgccgtctca ccaaaggaca cggcccttcg gattgaatcg
    56401 ccaaataatc cggtgagtga gtcggcagcc ctcccctcag aatccccccc aacttcggtc
    56461 gcgcctcggc ctgatccgaa gaccaatacg gcgccgctaa cagtggaggc agccgccttc
    56521 aatggggtga ccccgggtgt ttcccatgtg aatgacctga cgcgccaatg gggcccaccc
    56581 aaggaagccg tgacaaaagg ggattcggtg tattaccttt atgagattcc cccctttgac
    56641 cgaattgaag tccacatcca gaacgacaaa gtcgaagcga ttatcatccg gctggacaag
    56701 acttatcccg ctaatcttgt ggcggagcat ctggaattgt ctcaatttcg accggtgctc
    56761 gtctcgaatc cccagggcga ggtccttggc caaacgtttc ccgaacgtgg tgtcctcttc
    56821 agttttgctc ccgcaccaga acccggaaaa tcatccaatc tcgtgaccga gatcatcctg
    56881 tccgaggtga cagcagaacc gttcattctg cgggcggaga ccttttggca aacggagcct
    56941 gacagcgcta tccgtgacct ggaacaagca ttgaaattcg acaacagagc ccatcgcgcc
    57001 tggtggctgc tggcccgcat tcaaaaggaa ctgggaagtc tcaaagaggc cctggcgagt
    57061 gcccagaatg ccgtcaatct tgacccggca agtttgcact accgcctgac cttggcggag
    57121 attctcgatg aggtgggaca agataatctt gccattcagc aactcgagca ggtggtggct
    57181 gcgtcagatc aacgtccaca catcaaggcc cgggcattat gcaacctggg aaacatctac
    57241 cagtcgctcc cgcaacccga ctacaagaag gcgctcgatt accattatga ggccgtcaag
    57301 atcgcccaac ccctccgcag tgatgtacat ccggccattc gcgtggcggc taaagaggtc
    57361 ctcgtcgatg cccacctcgg ggcagccctg gacattgcat ggggaccctg ggagcaaaag
    57421 gaggcagttg tcccacgatg gctggagcgc gccgctaatt tagctcagga tttggtcaac
    57481 accgagcagc aatccgcaga taagctattc cgcgtctcga caacggccct cgctgccagc
    57541 gtggccatgc cgcgggtaat tggtcccctc ccttggatcg aagcattgga ccaagcctac
    57601 caacggcttg aagcggaagc gagtgaagtc cgaagggctc gtctgcgatg gaattacggg
    57661 gttgccctgt acgacgcggt ccagtgcttc caggtactcg gtgaacagga actggcccaa
    57721 caatacggaa aaaaggccgc cgaggccctg gaaaccggcc tccaaggacg ctcacccaaa
    57781 ggtagcgact tgtacttgct tggccgactg tatttcagga tgggcgctgt ggatgcgatc
    57841 caaaacaagg atcacaaaca agccgtcact tatttcgaca aggccattcc tctcctccgt
    57901 caagccgcgg gcgatatacc tgagacctca atcggacggc tgggagagac ttttgtcagc
    57961 atgggggtct cctattggga aaccggcgac cgggatcaag cgatcgcact gaccgaggaa
    58021 ggcctccagt ggatggaaaa ggcggttcaa ctcggcctac tcgccaattc tgccttagac
    58081 gtgccgaggg cgaatttaca gacaatgcgt caagctcgcc agagactggc cgagaggaca
    58141 tcttcggata ccgcggctac gcagcgatag actcagcccc cttcgcacaa gcagccctct
    58201 gaaaataaaa tcagcacttt cccaagtgaa caggctgtcg agttcatgcc aatttggcag
    58261 ttctttcact ccctcgacgc aagcatcttc ccacagtaac tggcaagcgc tgtgtctacc
    58321 taagttatta ttggaaaaca acttacgagc ttgtgaaggc attgtaaccg cgggaaaaag
    58381 ttgctctggg gacttttggc acgcggattg catttaaatc cgacggctga tcaacgaagt
    58441 cctcttcact acggagctct ctacgaaatc tggtcgggtg gtagcctcga tttgggatgc
    58501 caccggctga atcgaggcaa agggggatgc tcggtggcga cggtaagcga tagcaggagg
    58561 tgtggccatg acaaggaccc tggtaccctg gaccactagc ccaagccggt tgttcgaatc
    58621 actccgtcgc gaaatgaatg aattgatgga tcaattcttc ggcagcgacg ggggaaccga
    58681 tctcaccctc tggtttgctc cgcggatcaa cctcgccgaa acggatcagg cgtttgaagt
    58741 gacggttgat ctgcccggaa tgaaaccgga ggatttctcg gtcgagctga aggagggcca
    58801 actctggatt accggcgaac gccgtcagga aaccgaggaa aagggcaaga cctatcaccg
    58861 tgtggaacgg caatatggcc ggttccaacg ggtaatccca ctgccggcag ccgtcaatcc
    58921 agagaagatc gaggcccagt acaaggacgg tgttctgcat atcaccctgc ccaaggacga
    58981 gaccgcccaa ccgaaacgga tcccagtcaa gacctgatct gggtccgctg ggttgggtta
    59041 aagggcaagg agtgcctatc atccccgacg gggtgggcaa ggatggaccc ttgtaaaggg
    59101 cagcctccta tgcagcccag gcgccctgta gctcgccgca gcgacttggc aatcctcctt
    59161 gacggtcccg tgtaaacagg aatatggccc accccggggg cctttttctt ttttgaacct
    59221 ttgcgggatg gtattttcca cacaggacgc cttttttggc aatttgtgga aaagcgatct
    59281 caggttgcct tttccgaact ttagctgctg tattgaagtc acctgtcgtc aagtttccct
    59341 tcaagtgggc aatatttaag tgatgggtcg agaaacgggg caggataggc aggcctatct
    59401 cgttcgacga ggccatttct tcccggtgca gttgccgggt caggtgagag aacctcgttt
    59461 cgcacctgtt tccagctgga tcgccacaac atgccgcgaa actcggcgcg gcgaagtccg
    59521 gtgggagttc tgcctgtagt acagagaggg tgttcatctt gtcagatgaa ccgcggaacg
    59581 atctgtgcaa agactcacca ggtaaagact ggccaaggtg cgatacagca gtagtcgatc
    59641 gaaggtgcat cgccccaaga aaatgaaata acgagcttgg cggttttcga ctctcagcgt
    59701 atgatatgac gaaatacacg cggcgacgct tctccaccga accgaagtgg gccgtcatct
    59761 ctcctatttc cggaacggtg gatctgccga ttcgattcag tgacgtacat gcagcaaggc
    59821 ccgctcgccc gtttgagtgg gcttgatttc cattcatagc caactgtgcg gcttgcccca
    59881 aacacgtatg agtcgtgatt atctctctct gctccgtgtg agcattcatc cccgacgcca
    59941 acaaaacccc ccgacatccg acaacggtcg aaggattgct tcctctgtcc tgcccgctgc
    60001 cggttcactc ccatcatcag tctcgccacg atgcatctac gacgtgacgt tctggttggc
    60061 gtatctctcg aatgctttgg tgtcggccgg caacgtcctg ctctgccgat atgccgatta
    60121 cgtgaacttc cttggggctg atgagtacca ccttggctgg attattggct ttggaatggt
    60181 gggcagccta ttcatgcggt ttgccttagg cagtggaatt gaccgctttg gggcacgtcg
    60241 cgtctggctc ctcacgatcc tcgccactgc cctaggatgc ctcatccatc tgggaatcac
    60301 cgactatcgt ggtatcgcca tctatttggc ccgcgttttt tacatcattt ccctggccgg
    60361 tgtctttagt gcctccatga ctctcatagc tgcccgcgct ccccgtgagc gactcgcaga
    60421 ggtggtcggc atctacggtt ccgccgggtt tgcaggagtc ctcttaggtg caccgctggc
    60481 tgacttgctc tttcctgcgg gaatcacctc gatgggcgag gc
//
LOCUS       DAOSUT010000003        59866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_354_length_59866_cov_9.584525, whole genome shotgun sequence.
ACCESSION   DAOSUT010000003 DAOSUT010000000
VERSION     DAOSUT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 59866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59866
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_59866_cov_9.584525"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..874)
                     /locus_tag="PLD05_00470"
     CDS             complement(182..874)
                     /locus_tag="PLD05_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOP75926.1"
                     /translation="MRLTFKVDRSLLIGLALMALSPAAFAVDLSLAVWAKEDAYPKLL
                     VKASNLSEFFAHGLGVLCIVFLVGLLDTKGWRRGMTVLIGAFSAGLTADLMKLLIFRV
                     RPRDFDFAGGILTTFQEWLPVATRQFTHLSSSFPSSHAATAAGLAFVLILLYPKAKPI
                     FTVLLVLSCAQRIVSSAHFLSDVLFGAGLGWCVSCLVVWYLVPSSWWSDQAPASRLGR
                     PAQRDESKGEAT"
     gene            1105..1836
                     /gene="bshB1"
                     /locus_tag="PLD05_00475"
     CDS             1105..1836
                     /gene="bshB1"
                     /locus_tag="PLD05_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOP75927.1"
                     /translation="MLLKDQDGISHVDSVVVAPHPDDAELGMGGTILKMVASGLTVGI
                     VDVTNGEPTPYGTPEIRAAETEQASRVLGIRWRINLGMPNRSLQPTLEGRAALAAVFR
                     LSRPKAVFCPYWEDAHPDHLATTEMTEAARFWAKLTKTDLPGDPYYPPRMYYYFCTHL
                     RKVFVPSFAVDITPFIDKKLEAVRCYESQVIMGRPTDPYSFVDSLRHQAAALGTAAGV
                     GFAEGFACRETLLVDDLRALFPVPR"
     gene            2346..3686
                     /locus_tag="PLD05_00480"
     CDS             2346..3686
                     /locus_tag="PLD05_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75928.1"
                     /translation="MNQSPPEDQTTAASAEELGEDMESSSALISRYSFEIRVGLAILG
                     VLGFIFAAVVAYRMIKLRQDEVNRTKPTETAQAGDPTESQTSGQSHSSAQEEASGESI
                     APPPPFALDFPNSFPSSAQSASSGATEASHPVRNQRAAQGWVLPPDGPAEESPLGAAT
                     STNDPATGYAAIGDSAGTASSCAQLTPAAPVAGNTPPGFSNDSSPLYGAGQNNSSAPI
                     HASDARVPDFFAENRSIQPSSLTPGPPIDGSPNQPGPFAGSTSRQNLEAESGIPFPGD
                     FFAGGAALSSQGNLGGLPSSVQPAGQDPQPTSNDNRFATNNVTPTVDPFSRSSGVVNP
                     RSQEASAGVPQGGVATTQGPSPYSPPLVASESNVGENPDPMAGNYITHTVQEGETLFD
                     IARQRLGKASRWVDIYQLNRGQLGERLEYFRPGLILRLPADATHSTATSPTTLR"
     gene            complement(3730..5100)
                     /locus_tag="PLD05_00485"
     CDS             complement(3730..5100)
                     /locus_tag="PLD05_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOP75929.1"
                     /translation="MFSSALRNDALAIWHAGLDAVKADRLVAENICVEGSRLLIGKNA
                     DLVIELDRVDRLLVIGVGKAGAGMVRGLEKAIPDNLAAEKQLTGWVNVPEDCVGPTRW
                     IRLHPARPAGVNEPTESGIYGTERILELVRSAGPKDVCLCLLSGGGSALLPAPIEGIT
                     LQDKQKVTQVLSAAGANIAELNTVRKQLSKVKGGKLLRQCRAGWLITLIISDVLGDPL
                     EVIASGPTVPDPTTPGEALDVLHRYCGQQGQIPQHIFHVLEKRAANQPSETSDSITTQ
                     AFHLVIGNNQTAVEAAGRKAVELGYMVTSRTAATLEGLAEDVGRFLVREALEQMRRPG
                     RHCLISGGEPVVRLAPPEIRGRGGRNQQLVLAAAEMLWNDAPEGLILLSAGTDGEDGP
                     TDAAGAFVDAAIIKAAKERGMQPRFYLERNDAFHFFEPLGGLIRTGPTHTNVCDLRVV
                     LTDRPE"
     gene            complement(5120..>6046)
                     /locus_tag="PLD05_00490"
     CDS             complement(5120..>6046)
                     /locus_tag="PLD05_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP75930.1"
                     /translation="SWRWNGGMRGRKGSTDEGGVRVPCVIRWPKHIPAGLRISQIAAA
                     IDLFPTLVSLAGIKANPLKPLDGWDLSPLLLGKQTAWPDRLIFSHQAGRVSVRNQRYM
                     LDAQGRLYDLQTDPGQTRDITKELPDVAEKLQAAVNEFRATALPQTPDDRPFPVGYRE
                     FPVTFLPARDGVPHGNIRRSANAPNCSFFTNWLSVDDAIIWDIEVHTAGTYEVCVFYT
                     CREKDVGVELEVSFGSASVKGQVTEAFDPPLRGQEFDRVPREGESYVKDFKPLLFGTL
                     TLPAERGQLSIRATKIPGERAIDLRMVTLTLK"
     assembly_gap    6047..6146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6147..6670)
                     /locus_tag="PLD05_00495"
     CDS             complement(<6147..6670)
                     /locus_tag="PLD05_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP75931.1"
                     /translation="MVLLLWGFYGEATPAKQPNIVVILSDDQGWGDLSLHGNPNLQTP
                     NIDRLAHEGAMFERFYVSPLCAPTRAEFLTGRYHPRTGVHGVTTGAERLNLDEYTVAE
                     AFRAANYRTAIFGKWHNGSQYPYHPLARGFQEFYGFTSGHWGIYFDPPLEHNGKIVTG
                     KGYITDDLTDHAID"
     gene            complement(6770..8863)
                     /locus_tag="PLD05_00500"
     CDS             complement(6770..8863)
                     /locus_tag="PLD05_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOP75932.1"
                     /translation="MEILAPANDFACLEAAVEAGADAVYFGLPHLNARRRARNFTPDE
                     AARAVQFLHSHGRKAFLTLNTDISYRELGLALSTLEQANEIGFDAVLVRDPALLALRP
                     AYPKLEFHFSTQTTIANSVDVEAARELGADRVVLARELDLDEIRAACSVPGIAVEVFA
                     HGALCFSISGRCLMSSWVGGRSGNRGVCASPCRVPWQVDGEPAGFPLSMHDLCILEHL
                     SELSAAGVSALKIEGRLKSAQWVKRAVSLYRQALDDTADKNSLLSLAKALTEYAGRDL
                     TDGYLLGRRTNLTGTNRGRVGRDETSLIEEDGDLADSTTDVSCSENGEVFDSSAGSVV
                     FHLEVTGPRIRCNLEVEGKDISWEYPKTVVRRVHKALSAQEVCQQIEGRRFQGIFLDA
                     AETDDPDFLIVPRTANAILDRITAEMARIQRAKNRPLDIKIPEGARVSADDLCPATAN
                     NRTLGDEPNRVRLSFLQLEAFLREDPKVHVIVEDVNPEDVSAVAQMCRKVPAVIALPP
                     VFFERDIPRIEETINRCKKARLGVEVNTWGGWLLARRAGVAFETGPGLGVLNPLAAAY
                     LRKLGARCVAVSVEADRRQIEDLCANASVGLSLYVMGRPPLAISRAELPEELLGHVFT
                     DRRDLRMIPRRQWGLWVFRPEYPFDWRVLKNQRIHVRHLVVDLVGSPDPVNEWRHLTS
                     HREKRFYFNYNRVLQ"
     gene            complement(8988..10622)
                     /locus_tag="PLD05_00505"
     CDS             complement(8988..10622)
                     /locus_tag="PLD05_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP75933.1"
                     /translation="MYPTLEKADHEVGLALQEAKDEGTVPVDNLTSAVATYLPQAVFI
                     VDEKFRVVWANNRASELLPAATPLVGKQFYQDLFRDVQLIRPEFFPLTLARDTGRPAR
                     SVMRLKGPRFFELFASRIPQTEEDRVRLIVSMQEITSTFLPLERLRAIHEAGRHLTEF
                     APEFVTRYSAEERKEVLKRRVLDYLKKVLHFDVFEIRLIDPQTKELVPFISDGMSPEA
                     AQRRLFVGLQGQGITGYVAATGASYICEDTASDALYLKGTEESRSSLTVPLIRHEGPS
                     RAVVIGTVNIESPRPCAFSHDDRMFLEIFCRDLVVALTTLDLIEAEANVATRGLVEAI
                     HGRVALPVDQIVSKAAEADAYLDHDPARCDVEKARAILKEIHKIARDLRTEIRSFGAR
                     LPPTEASPIFQNKRVLVVDPDESIQRDAHKYLEQWGCVVETASTGRAGIQMAVMTAGE
                     GGYDVILGAHRLPDMKGWEFFKQLKEALKAEKLPYVMLCPIGQHDADHNFVKARELGA
                     EYQVGKQPPPPGQTSGGNLVVKPLVNILNRIFGVTT"
     gene            11150..11395
                     /locus_tag="PLD05_00510"
     CDS             11150..11395
                     /locus_tag="PLD05_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75934.1"
                     /translation="MSRAFFLAIGLFVVFLGVQTLGVQRFVLKTRDPAPPGNVAIGES
                     QPGPNKVLEPPPWAPWSLMATGAIVSLYAVTLPTVRK"
     gene            11438..11854
                     /locus_tag="PLD05_00515"
     CDS             11438..11854
                     /locus_tag="PLD05_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOP75935.1"
                     /translation="MPVYREKLRYTTQGHGHMLDLTQDVAKVCNRAGVSDGLVNLFVV
                     GSTAAIGTIEFEPGLLKDFPNLLDKLAPPGRNYAHEATWHDGNGHSHLQATLLGPSLT
                     IPLESGALALGQWQQVFLIECDVRSRQRTVIVSVLS"
     gene            12054..12272
                     /gene="csrA"
                     /locus_tag="PLD05_00520"
     CDS             12054..12272
                     /gene="csrA"
                     /locus_tag="PLD05_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOP75936.1"
                     /translation="MLVLSRKCNEQIVIGDDIIVTVVSIRGGTVRLGIEAPPHVTVHR
                     LEVLQAIRADEAARMKDSSNNSSPPTTP"
     gene            12524..>12864
                     /locus_tag="PLD05_00525"
     CDS             12524..>12864
                     /locus_tag="PLD05_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOP75937.1"
                     /translation="MSPVVTATAIPGLQKRSGKVRDIYDLGEQLLLVSTDRISAFDWV
                     LPNGIPNKGQVLNLLSAFWFGILGEPNHLITADVDSMPLPPGADKETLRGRTSLVKKT
                     RVVPIECVVRG"
     assembly_gap    12865..12964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12965..13189
                     /locus_tag="PLD05_00530"
     CDS             <12965..13189
                     /locus_tag="PLD05_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOP75938.1"
                     /translation="EVLTPDSSRFWPADTYEPGKPQPSFDKQFVRDWLESTKWDKNSP
                     PPALPEDIVIKTRQKYIEAYERLTGTAFVV"
     gene            13232..13555
                     /locus_tag="PLD05_00535"
     CDS             13232..13555
                     /locus_tag="PLD05_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75939.1"
                     /translation="MPLYEIETEYHIIITWADNEDEAKLVVRDSYPREAILRITRRPR
                     NSWVISKAALGMTGRTDPCNIARDCLAKAAGDKIHAIRLYMQETGVDLEKARKVIESN
                     MIMGW"
     gene            14392..16023
                     /locus_tag="PLD05_00540"
     CDS             14392..16023
                     /locus_tag="PLD05_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75940.1"
                     /translation="MAIDFLTECLSELEKTQVALGDQMALEAVKQLYTARIYELRGRL
                     QASMASLLEAVGSDRDRQPVFPDDIPHTGGYRTQLEKLFPETPPLGLRRLNEMIPLAR
                     QSIYLHFQASQSAYDAWLACKEAYDAQRGDLWSALACWRLWVDEQLHCAEAIENYNRL
                     ILEYVITSGNGQLSAEDFVNMLVEQDARRVVSGQRTSANRPDQMPSKGTSEATKTPSD
                     PQTRQFPSFFQHVINDPAKPSPPLGEESPFLLIWSTSRKPWPLIPVSLSNENGPAVDA
                     GLSTPYFGKLQQGSAGVFVRQVAELSFKRGVTAAMQDGLNTDIAMDEKLLSGGDFAHR
                     ARLYWDVAVVQAEYHVFLQYSAILSELFPTVLARWNEPGAAEEMLQLQAFRRTVDAGL
                     QDIHLKMLDRGSDLIALNSKSDNAHPVAITLPYVGEYDTRWDQIRTVLPEPTRTAAMR
                     VVSRIPATYAALCQNALALNAADQARVFAVGRFLNKQERIGPVLSALHFEHEAWLGFI
                     ELTGQYNQLIAEYVSLVRPDLDQKSFLSAIGITQR"
     gene            16212..17162
                     /locus_tag="PLD05_00545"
     CDS             16212..17162
                     /locus_tag="PLD05_00545"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOP75941.1"
                     /translation="MAGYTNYAFRQLLRSLGGVGLLCTEMVSARGFLELDRRYQRLPD
                     RLWGVRDEPRPIAVQIWDNDPHKLAMVAERLVEDFDVSCLDINFGCPAEQITRGVACG
                     AYLLRQPDDIGEIVSAVVKRVSPVPVTAKIRLGWSDQEINAPAVAEVIQQAGGAAVFV
                     HGRTAQQRYRGTADWSQIAAVKKRVQRIPVIGNGDIRSAQQAVTAMREFGVDGVMIGR
                     VALARPWIFREAAALLAGLEPPPEPPAEEQYEMLLELYRQLESYFGSVKAGILMRSFA
                     SRFVTARRGAKRFRVLIHSSKSSADLLAHLRDFFLHNPES"
     gene            complement(17221..17913)
                     /locus_tag="PLD05_00550"
     CDS             complement(17221..17913)
                     /locus_tag="PLD05_00550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOP75942.1"
                     /translation="MSGLTIAIGDIHGYDVALRAVLDQIGPSGSDTVITLGDYVDRGP
                     NSREVLEILIELQGQVHLVPILGNHDEMMLSIWQGDHHLFDDWLHYGGAATLESYGLT
                     TLDGVPEHHIRFLQECRTYLETPTHIFLHANYIENRPLSEQDVFTLRWESLRQRLPGP
                     HRSGKKAIVGHTAQRNFEILDLGYLVCIDTCCYGGGWLTALDVESGQVWQADAQGRTR
                     KWVLDSFNTSPG"
     gene            complement(17989..18951)
                     /locus_tag="PLD05_00555"
     CDS             complement(17989..18951)
                     /locus_tag="PLD05_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOP75943.1"
                     /translation="MNDTVTWGLLGAVLVLLIIATVVVQLLRRSRPEVINSEIVESFS
                     GRLRAWWLLFAGLAGAIVLGKTATVVFFGLISFWALREFITLTPTRPSDHRALFWVFV
                     LCTPAQYVLVGYAQYDLFAIIIPVYALLFLHTRIAFSNDPVRFLERTAKIQMGLLICV
                     YCLSYAPALLTTLELGNDAYNLRLLFFLVFITQLSDALQFAWSQLPSRHVVVPNINPT
                     RTWEGLLGGSASVTLIGAMLWWALPFNSIWITAGLSLVISLVAFAGGITMSAIKRDRG
                     VKDYGTLVAGHTGVLDRIDSLCFAAPVFFHLTQIYLALQPAASA"
     gene            complement(19046..20650)
                     /locus_tag="PLD05_00560"
     CDS             complement(19046..20650)
                     /locus_tag="PLD05_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP75944.1"
                     /translation="MAKSVEGKEVGKRTVLYFHQKDWIMSVFVHRQASESAVVQRPRR
                     HFWLICCVFWAATCPWLGQPILGETEEEAVQHAKYLSIAFRSAARKVLPAVVTIKTAT
                     SKEGQEAGRGGPRLPWEEFPKPSEPGFQNEIPRNPDLGMGTGVIIDPKGIIITNRHVV
                     EGGDVVTVRLVDGREFPVIDKKSDELSDLAILKIEAAEDLPAARLGDSDKLDIGDWVI
                     AIGNPFELEGTVSAGIVSAKGRTLRSIGRVRFIQTDAAINPGNSGGPLVNLDGEVVGI
                     NTAIFSRSGGYQGVGFAIPINLVKWVSSELLAHGRVRRAYLGVTLEDVLLPRKAVSGE
                     ERQSLGGVVVRQVIRETPADRAGLRRNDVILSFDGVDVSDVASLQEIVERASLDVPHK
                     VRVLRDGTSIELEIQLQESLSERELAERLKQQNESQDQPRRKRVYDKELGLMLMDFDP
                     ALAPDTGVRVTHGVVVIHADPGRPGARAGLSTGMVIVKVNDRVINNLAEFQEAIKAAD
                     SKAGIKLEVVSRLGRQILNLGVGEKK"
     gene            21004..21912
                     /locus_tag="PLD05_00565"
     CDS             21004..21912
                     /locus_tag="PLD05_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP75945.1"
                     /translation="MKTNYRRRAFLMGSSALAIGAMIPWRFGVLNAATVEKLAEGAPH
                     AEKLGWRLGCQAYSFNRFTFYEAVEKTAAVGLRYIEAYPGQRLSQEKPDVKTDHNLSV
                     QDRLEVKKRLADAGVTLVNYGVVRLSADEGENRKVFDFAKDMGIETIVSEPPFDAFDV
                     IEKLCEEYQINVALHNHPQPSIYWNPDTVLKVCEGRSKRIGACADTGHWMRSNINPLE
                     AIKKLEGRIISFHFKDLNDYGRGAHDVPWGTGKADVPALLREIKRQGIKAVFSIEYEY
                     NWENSLPEIAECVKFFDKVAAELLAQ"
     gene            22388..22864
                     /locus_tag="PLD05_00570"
     CDS             22388..22864
                     /locus_tag="PLD05_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75946.1"
                     /translation="MDREESQSTFLRHCALVEATAERLAAEGVGWTTFFRELLGPEGL
                     VRQLFPTPQQLSEFERTDTYQKVQEILAKLRRLQEGRSAPDEPLTVITIRVPRSLHEA
                     LAEEAHALKTSINRLCISKLAQLIDTRFVPPMRHLDREASHEEQELTQPPSRKTRK"
     gene            complement(22881..23284)
                     /gene="rnpB"
                     /locus_tag="PLD05_00575"
     ncRNA           complement(22881..23284)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD05_00575"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23344..24375)
                     /locus_tag="PLD05_00580"
     CDS             complement(23344..24375)
                     /locus_tag="PLD05_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOP75947.1"
                     /translation="MGTNPDKRLGINSASSYNWRQLGYRYYSLSFYLNRIFGGKTWKV
                     SVDGGFHCPNADGTISHAGCLFCNILSFSPSRRRSGLTIREQLLQAATHLRERRGIQK
                     IIAYFQPGTNTYGPPDKLERLYREALSVPDVVGLAIGTRPDCLSEEVVKLLATLSQET
                     WLTVEIGVQSSHNATLRRINRGHSWEDAVNAIQRCANEGIRVTVHLMFGLPGETYQQM
                     LETAEKVAELPIHGVKIHNLYVAKDTGLAKLYEAGQYEPLSQDVYVNMVCDALERLPP
                     HIVIERLTAETDPGYLIAPDWCRRKGETISLIGKQLELRNTYQGCRFSEGPTQPDGLS
                     SSPNKCGST"
     gene            24485..25000
                     /locus_tag="PLD05_00585"
     CDS             24485..25000
                     /locus_tag="PLD05_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP75948.1"
                     /translation="MYGELIPLGGGDPIPLLKKRLIVGRRESCDIVLRFSNVSAQHCE
                     LFVMSGYWYVRDLNSTNGIKVNGTRVREKRLDPGAVLSIAKHQYEIRYNPIELGAVGP
                     PPPDTLEQDIFTRSLLERAGLVKSKPVPEPEETEKTEPPSGDDGKRYDILEGFRKSEF
                     KKSWTEPQSPK"
     gene            25087..26250
                     /gene="rsgA"
                     /locus_tag="PLD05_00590"
     CDS             25087..26250
                     /gene="rsgA"
                     /locus_tag="PLD05_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOP75949.1"
                     /translation="MGKKKKIRVDFRKNRTSKPRQKDLTDRVVSRDEVLDEDLTREER
                     IGGRGELTRRRTVIGQETDDKSGVATPLPVILEPRHPDSRLGRVIAVQGLICQVEDAE
                     GQAYRCVIRRLLKRLATDQRNVVVVGDQVHFHPLPGSDGEGVIETILPRRGALARTSK
                     GKHHVLVANVDQAIIVSSAAMPRLKPHLIDRMIVAAEKSRIEPVICINKIDLVDPADL
                     EPLVGLYSQLGYRVLLVSAITGFGLPALIRTLKDKVSVLSGQSGVGKSSLLNAIDPSL
                     RIPVAPVSEDTEKGRHTTTTARLYRFHFGGYVADTPGIRQFQLWDLTPDEVVNYFRDL
                     RPYVNGCRFPNCLHLEEADCAVKDAVADGLIDERRYESYCHLVLDEEVEEEEE"
     gene            26442..27800
                     /gene="hflX"
                     /locus_tag="PLD05_00595"
     CDS             26442..27800
                     /gene="hflX"
                     /locus_tag="PLD05_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOP75950.1"
                     /translation="MRPRNGSQPLGEKPERGVLVGVQLPTSVLEDPPLAELAGLAKAA
                     GVEVVGQVTQRRMAPDAATYIGSGKLEDLKKLVQSQKADVVIFDNDLSPAQGRNIEKV
                     VRARVIDRTQLILDIFARRAQTYEARLAVELAQLEYTLPRLKRMWTHLERQTKGGVGL
                     RGPGEKQIEMDRRLISHRIHQLRSELEVVHQRRRREVIARKEFRTVSLVGYTNAGKSS
                     LMNALTDAGVYVEDALFATLDTRTRRWEIPGWGPVLLSDTVGFIRDLPHHLIASFKAT
                     LEEARQADLLIHVADGSNPAVFEQISSAYKVLAEIGIESKDTILVINKIDRIDDPSRL
                     HCILDRYPNAVLVSAKTGEGLQRLTLMVSELLARQFVEVDIETNVGNGKLIAFLMTHG
                     ELIHERYHDGDRVTFRCRLPGQYLPIVEKLMEYRPDGNDQSQSNREMAAYQIHSAGMV
                     SE"
     gene            27864..>28615
                     /locus_tag="PLD05_00600"
     CDS             27864..>28615
                     /locus_tag="PLD05_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOP75951.1"
                     /translation="MSHRTLNGIRGVVTGASSGIGQAFVKALLPFRPRLLVVARREER
                     LQQLSQLGAQYGAVVIPFACDVSQPEAAKRICEVAHQNFEGIDLLVNNAGIGALGRFE
                     DSPPEELRQVINVNLFSLVETTYYLLPLLKKGRRPIIVNVSSILGHRGIPWRSYYCAS
                     KFAVRGFSEAIRAELARHGIDVLVVSPGRTATELFEGPLEKGKTPAWPDPKPVSAEYV
                     AQRMLRAIQRGRHEIIPHPWGRLMVLVSRLAPR"
     assembly_gap    28616..28715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28716..29291
                     /locus_tag="PLD05_00605"
     CDS             <28716..29291
                     /locus_tag="PLD05_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75952.1"
                     /translation="EDSAGKKVRTELVRRLLAKLDSLDDISAEDKKKLERILLEADAE
                     LAQAADRPRATGRGFRSGDRPTKGQPPQVGQEPSREQGAVTARGAQTPSTQASEGQGQ
                     PETPTEAETPMVRALLKLLDEVIADNLADNSDDSQSADALRESLLRSDQVLRQYLGHA
                     ARARIEAANRKPPVPQVSNPRKKPSSDVEQK"
     gene            29517..30527
                     /locus_tag="PLD05_00610"
     CDS             29517..30527
                     /locus_tag="PLD05_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP75953.1"
                     /translation="MTRQTRGYLSILILVCFVVGCRSYPWSPESVAQGPLEGQSRSTL
                     AYDDQSKTAQAAPKKGSKAQAEEEAEYARKLAQGRSLERLGKLDEARKTYEGLIARFP
                     TRYEAYHRLAVVSDRQMRYTEAEALYAQAIRLNPTNPDLFNDLGYCYLLQGKPEKAER
                     ALLKAVGMAPASERYRNNLGLAYGLQGRYEEAMEQFRRANGEADAWYNLAYVQWMRND
                     IHLSKQSLENALAYNPSHTAAKESLAQLVSGQSFFAESTGERRSGYRLAPDTESELIA
                     ANGAPSSSDQSVTQTTFEMVPGTLRLGLKDRPSTQTQLDVARSSWAKRLQQETAPLEL
                     AE"
     gene            complement(30545..31345)
                     /locus_tag="PLD05_00615"
     CDS             complement(30545..31345)
                     /locus_tag="PLD05_00615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOP75954.1"
                     /translation="MMKATMSNVFAELVLVILVAFVTEKVSAAENGPSTLGKNDPVLE
                     RIDFIYSEMVPLSWTPPSDRWRNLERSIATLKHGGTLRVVMLGDSIVNDTSRSAWEKL
                     VARLYPGAHIEKITSVRGSTGCWYYKDPNQLEEYVLKYKPDLLMIGGISHRDDIESVR
                     EVIRGVRANLEPDPEILLMTPCFGHKDPRMDPEWTFEVPKDGNDFRSRLKRLAEEENT
                     AFLDMTGPWGQYIRTCGEDIHWFKRDIVHANARGEAILGRILEKFFAP"
     gene            complement(31694..34105)
                     /locus_tag="PLD05_00620"
     CDS             complement(31694..34105)
                     /locus_tag="PLD05_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOP75955.1"
                     /translation="MNVKAMTFLLGKTLLLIGLLIARDAGSGTAFASNPLVLWYNSPA
                     EKWTEAVPIGNGRLGAMVFGKTDCERIQINEDTVWAGSPHDYSHPGAAKYLPEIRRLL
                     FEGKQEEATALAMEHFMSVPLRQMPYQPCCDLYLDFGENAKVSSYRRELDLSSATAIT
                     SYQVDGVTYKQTVFASYPDQVIVVRVTADKPGSVGLKASLKTPHVEHAIQTASNAIIL
                     RGKVSDYIDKRTKETMPSAIRFEVRALVQPEGGSLVTHDEALQVSGAHAVTILLTAAT
                     NYRDFEDVSADPSARCAEILEKARTRSYEELLARHMADYRSLFDRVQFALPSTEAAQQ
                     PTNLRIRNFDKQDDPELCVLYFQFGRYLLISSSRPGTQPANLQGIWNESLSPPWESKY
                     TANINVEMNYWPAEVCNLSECAEPLFDAIEEIAISGARTAKVHYDAPGWVLHHNFDLW
                     RGTAPINAANHGIWPTGGAWLCQHIWWHYEYTQDRDFLAKRGYPLMKGACQFFMHYLV
                     EDPRSPQRWLISGPSNSPEQGGLVMGPTMDHQIIRYLFATTAKAAEILGVDADFRKRL
                     MEMHARIAPNQIGEFGQLQEWLEDKDDPNNKHRHLSHLWGLFPGEEITLRGSPELCQA
                     ARRSLEFRGDGGTGWSKAWKISLWARLADGDRAYKLLSELISQSTLPNMLDNCPPFQI
                     DGNFGGTAGIAEMLLQSHGGEINLLPALPAAWETGSIAGLRARGKVGVSIRWDKQSLE
                     AQLNPEVPQVIRLRVPEKFTKVTIFDERGVPVEVGVTEDGARTFSAKAQTYIVRASLE
                     YEPIP"
     gene            complement(34589..34671)
                     /locus_tag="PLD05_00625"
     tRNA            complement(34589..34671)
                     /locus_tag="PLD05_00625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34636..34638),aa:Leu,seq:caa)
     gene            34979..37747
                     /gene="gyrA"
                     /locus_tag="PLD05_00630"
     CDS             34979..37747
                     /gene="gyrA"
                     /locus_tag="PLD05_00630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOP75956.1"
                     /translation="MSDTEFEENDNSTGTPGGQSPQGETRGRLIPLAIEDELKSSYLT
                     YAMSVIVSRALPDVRDGLKPSQRRILVAMNDLNLGPGAARVKCAKICGDTSGNYHPHG
                     EGVVYPTLVRMAQEWNMRYVLIDKQGNFGSIAGLPPAAMRYTEARLSPYAAMMLEDLN
                     LDTVDFVPTYDERHLEPTVLPSKFPNLLVNGATGIAVGMSTEIPPHNLSEICDALVRL
                     IDDPDVTIDELLEIVPGPDFPTGGIICGRRGIRQGYLTGHGTIVIRARADIEEYRDNR
                     YRIVIREIPFQQQRDKIEEKIAELVNEGRISGIATIRNESDLKEPVRIVLELKRDADP
                     EIVLNQLYEYSPLQQTYSINLLALVDGKPRLLNFKELLQEFLRHRVTVIRRRTQYLLG
                     KARQRKHTVEGLLLALANIDEVIQVIRTSETQAEAKERLMKIACPAAMLARALGEAGF
                     EQFRSERGVSEEYFLTAVQADAILRMTLGQLVNLEQEKLAEEHRRLLADIAEYNRILS
                     DERNILAIIREEQLAIKQKYGDARRTEIIDQEVGSFDMEELIPDEMMVVSISSAGYIK
                     RTPVSAYRAQRRGGKGLKGARADDEDPIEHVFLASTHDYLLFFTNLGRVHWRKVYELP
                     ELPRDAKGRAIVNLLNLQDNERVASCRPVRDFSLPNHYLLFVTMKGLVKKTELSAYGR
                     PVRGGIIAIKLREDDELVGVVITKPGDEIVLATANGMAIRFRESDVRPMGRAATGVKG
                     ITLAPNDRVVGVVVAEPDACLLTVCANGYGKRTPFGPNSEDEGEELHEDLDEVTEGTD
                     ELAAKSEELSEEDETDDDRDEPGSQYCYRTQRRGGKGIRDIKTGGRNGPVVGIVAVHD
                     DDEVLMMTAKGKIQRIRANEIRKTGRNAKGVRLMTLDEGDQIVAVKRIPAEPTNGSDA
                     EGNTPTTS"
     gene            37840..38799
                     /locus_tag="PLD05_00635"
     CDS             37840..38799
                     /locus_tag="PLD05_00635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOP75957.1"
                     /translation="MTHRALITGITGFVGRHLYAHLVECGDQVLGLAPSPVPKVPSWG
                     RLPDDGEILIWDFAESKECPDDIKARIRQFDPTVVYHLAAISVPEDCGHTAPNERAWA
                     INVEGTRRIIELLNETSSGPRFIFVSTSHLYRVPQDKVTFVDEEYPANPRNAYAQTKW
                     AAEQIVQAAVKEDRLNAIIVRAFPHSGPGQSERMMLPSWAAQFARGMQTIQVHTLQAT
                     IDLCDVRDVVRAYRLLADSAVPGEVYNIGSGVPRTSAQVFELLRLLANPHCEVVETHP
                     GIKYDPIADLRKVQSHTHWKPEIPPEQTVADTYRWWLATVGRK"
     gene            38915..40777
                     /locus_tag="PLD05_00640"
     CDS             38915..40777
                     /locus_tag="PLD05_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP75958.1"
                     /translation="MMKSNIVCFLPGAMLAISLAASTVAAETTIYLSPQGKDTNRGTA
                     DQPVATLARAMELVKAVRQRDGAAADVRVLLAEGIYRIAEPVTISPEHSPGPGKLVIC
                     GEAGKKVVISGGRVISGWQKVGNRFEVVIPEVAQGKWIFRELFVNGRRATRARSPNEG
                     FVRIDKAGPDRRTSLFIYPQDAWLFKGKISGAEFVYLHDWSTSRVKIAGFHPDTLEVT
                     FSQRVGCSAPHYAIGNWPHARYFVENSEDFLDAPGEWFLDDGTGKLIYLPREGESLET
                     LEAIAPVAPALFSIVGDLDHGKPVTNLTIEGITFEHCRFDLPAAGYAAGQATVYEPRV
                     NGGAGREIMPAAVTFDFAKNCQLTRCHFQHLGSSGVWFRRQCRQCTVERCVFDDISGN
                     GINIGETMTRPKSEAAGDWESPYDNGCTWGIMVRDNRITHCGRQFFEAVGVWVGIAAG
                     NVIEHNEIGYLPYTGVSVGWSWNDSPTGCRDNIVARNHIHHCMLILHDGGGIYTLGRQ
                     AGTKLLGNVIHDIPPNEDGAESNGIFMDEGSSDIVVEGQTIFNIGRSPLRFHRALEIT
                     LSHNTLVCLPGVPPYRYNRTDPKTLRFENDHIVEAEHWTPPSQILGEVGVREGR"
     gene            40810..42231
                     /locus_tag="PLD05_00645"
     CDS             40810..42231
                     /locus_tag="PLD05_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP75959.1"
                     /translation="MFRSRVVPLALVAASCVCHIGQVVHGEESSRGRYNVLFVIADDL
                     NTALSTYGHPMVKSPNVDRVQRWGMRFDRAYCQFPLCNPSRASFLTGLRPDHTRVYDN
                     AVHFRKNVPDCVTMPEFFRKQNYFVARVGKLFHYGVPGQIGTSGLDDPQSWDLVINPR
                     GRDKDEEDKIFSLEPGKFGGTLSWLAADGTDEEQTDGIGATEAIRLLEENKDKRFFLA
                     VGFYRPHTPYVAPKKYFSLYPPELVPLPTIPENYFSTIPALAITLKPEEKAMDEKTKR
                     QAIQAYYAAITFMDAQLGRVLDALERLHLRERTIIVMTSDHGYHMHEHGLWQKRTLFE
                     ESARVPLLIAVPGMKTAGQSTQAIVEMIDIYPTLVDLCGFEVPKNLDGMSLVPILNNP
                     AHPGKSHALTQVSRGGGKTRLVMGYSVRTDRWRYTEWDGGKQGRELYDHQDDPQEMKN
                     LAGDERYENVMKELSALMPKPAE"
     gene            42286..42693
                     /locus_tag="PLD05_00650"
     CDS             42286..42693
                     /locus_tag="PLD05_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOP75960.1"
                     /translation="MKKKVTLIAALLLALVGGLVVQLYAESQAKFSHAGQVRHVVLFK
                     FKDGTSPEDIAKIEKAFAALPSKIPQIAGFEWGTDCSVENLSQGFTHCFILTFKNVQD
                     RDAYLPHPEHKAFGQLLRPHLDKVLVIDYVVRE"
     gene            complement(42768..43811)
                     /gene="tsaD"
                     /locus_tag="PLD05_00655"
     CDS             complement(42768..43811)
                     /gene="tsaD"
                     /locus_tag="PLD05_00655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOP75961.1"
                     /translation="MPLFLTIETTCDETAAALVTDQLEVLSSVVASQDKLHERYGGVV
                     PELASRAHVERILPVIHETISRAGVKLDDITAVAVAYTPGLAGSLLIGLTAAKALCVA
                     LDRPLIAINHLQAHIYACRMAAGEDVFPCVGLVISGGHTCLYRCLNALDFELLGCTLD
                     DAAGEAFDKVAAMLKLPYPGGPSIDRAASGGDPKKYKFPRAFLDNPDCLDFSFSGLKT
                     AVRYAITGPGRHDCSQVDLPQQTIADLAASFQEAVVDCLVGKSLQALQATGLTTLCVG
                     GGVAANRRLRERLEEEARERNIRLIIAPLRLCTDNAVMGAIAVERWRAKLFAPLDLDV
                     EPGVVRRDTASRK"
     gene            43955..>44253
                     /locus_tag="PLD05_00660"
     CDS             43955..>44253
                     /locus_tag="PLD05_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOP75962.1"
                     /translation="MRASIQEVILGPLNPQQREAVQHGEQPLLIVAGAGTGKTATIVH
                     RVAWLVAHGVSPQSIMLLTFTRRAAEEMIRRASSLLSRLGLSRESTHTSHIWGGT"
     assembly_gap    44254..44353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <44354..45837
                     /locus_tag="PLD05_00665"
     CDS             <44354..45837
                     /locus_tag="PLD05_00665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOP75963.1"
                     /translation="DYDDLLMQWLKLLSHSQIAEHIRKRISFVLVDEYQDTNPIQAQI
                     LYALSPSGQGVTVVGDDAQSIYSFRGATVKNILDFPKHYPSARIVTLEQNYRSTMPIL
                     RAANAVMASAKERFTKNLWSEQRAGCLPRLVTCDDEYEQADLIIREVLAHHELGTDLR
                     RQAVLFRASHHSLPLEAELTRREIPYRKYGGLKFLEAAHIKDLLAFLRLAENPRDVVA
                     GVRVLCLLPGIGPAKAQKLLEQVASSPQPSFDPWKEINPSRDSADIWPSFVSVMEDVA
                     SRNLPVAAQIHRVRIFYEPMLPVLYDNPIPRAKDLEQLEQIASRYKDRQTFLAQIAID
                     PPNSTQDFAGKPDLNDDFLILSTIHSAKGLEWDVVYIINATDGHIPSDMATGSEAEIE
                     EERRLFYVAMTRARRVLYVYTPVRYYVSPSFADSRYGMGLPSRFLTPEVVECFERYHG
                     DVLDGEMQANYQQYPNDLPSGIGKPIFARRSQKAGGDISMESG"
     gene            complement(45884..46588)
                     /locus_tag="PLD05_00670"
     CDS             complement(45884..46588)
                     /locus_tag="PLD05_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75964.1"
                     /translation="MAIFVVCPGCRTRFTVSDQFAGRSGPCPKCKTIIQIPKLNEQVV
                     IEEPEQFADGGRGISGQLTTKPLARVETAIKPVHILIAVGAFVIVLVVTLVLGQLDIF
                     HQQVWLHAIGLVLVGVPAAAMSYEFLRHQDDLKPLRGRNLIIRSLACAVGYLIIWGLF
                     NWLVANFVTEELWTWAIVLPPAFAAGGFVAYAAMELDYSAGILHFTFFSLIALVLRWA
                     AGFGWIWNLPQPVYPT"
     gene            47173..49293
                     /locus_tag="PLD05_00675"
     CDS             47173..49293
                     /locus_tag="PLD05_00675"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOP75965.1"
                     /translation="MKVTRSALSVGRMQHFSQLAIGLLVIIWFAAPSEAQNRGLWTPD
                     QETGITADRATIIYEKLPDGRKVAHVQAKGVAENVAIRFRSQDGSWDLSDFCYVGIQV
                     QNRLEKEIRVQWRVRAKDKSLSRDYVMKPGESHFLRLTLRRAVPTQLKDVFFGMRGYP
                     ALMDPERGIAVNAVEELALNIRGPVEGTFLTIENVAASGKFQKPQWWNGITSRLFPMI
                     DMFGQYRHADWPGKTHSIEELRARIAEEDQDLAQHPGPSDWNQFGGWIGGPKLEAKGH
                     FWVTKHDGRWWLVDPEGCLFWSHGIDCVRATTATTPITDREHWFELPARDGPFGLFYS
                     RAGWAPHGYYQKRSPYEQFCFSGANLLRKYGENWEAAFNERAHRRLRSWGLNTIGNWS
                     DPAIYREKKTPYVVTLSSGKRKIEGSTGYWGQFPDPFDPTFDQPLREAMAREANQSVI
                     DPWCLGYFVDNELSWGDDVSLALATVRSPAEQPAKKAFMEMLKQKYGTVEKLNEVWGT
                     KYQSWENFLEVTDEPDRKKAESDLGAFYSEIAETYFRRCREAIKAVGPNKLYLGCRFA
                     WVNERAIRAAAKYCDIISFNRYEVSVASFTLPQGVDKPVIIGEFHFGALDRGMFHPGL
                     RETADQVERAEAYRRYVQSALDHPQIVGTHWFQYMDQATTGRGDGENYQIGFLDVCDT
                     PYPEIIQASRDVAAEMYRRRAHHQ"
     gene            complement(49482..50762)
                     /locus_tag="PLD05_00680"
     CDS             complement(49482..50762)
                     /locus_tag="PLD05_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP75966.1"
                     /translation="MRRLIFACLVSVTVGALAAVAAVKSGLTGGDRYMIDQPQDRQAA
                     FAEPWFPQGSEGPGSLPTWTASPTPWTVSTAQGQFVFTQSAPGWEAFLPPVGQYTPDE
                     IVNIAVYEKVNKSVVNIRTKSIQEGALFFMNIITEGEGSGSVIDRQGHILTNYHVVEN
                     ARAISVTLHDGMAYDAQLVGADPPSDIAVLKIDAPSEKLFPVTFGDSTNLKVGQRVFA
                     IGNPFGLERTLTTGIISSLNRSLPNPRSQRMLKQMIQIDAAINPGNSGGPLLDSHGLV
                     IGMNTAIASRTGESAGVGFAIPVNTIARVVPQLIRTGRVIRADAGILQVLETDQGILI
                     ASLKPGGAAERAGLQGFRLTKRTRRQGPLVFESTVIDRSTADLIIAVDGKPVKTVDDL
                     LTVLESKQPGEKAIFTIIRQGRQMDVPLILDAEN"
     gene            51535..52281
                     /locus_tag="PLD05_00685"
     CDS             51535..52281
                     /locus_tag="PLD05_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653816.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOP75967.1"
                     /translation="MRRKPLIGLNMDFGKVESKGYVAELPAGYFDCLIRAQAVPVAIP
                     PLVHRRDIEQALDAVDGFVLIGGDDLDPLRDGCYHHPTTRVMHPRREDFDRLLVKLIA
                     ERHMPVLGIGAGMQLLNVSQGGTLSLNIRDDFSNAMDHRDPHDPYHRHGLVVSPKTFA
                     KRVYGDGEIRVASRHHMAVLDLAPIFKVSAVAPDGIIEVIESQDPDWFAIGVQYHPEC
                     EAASALDLRVFEEFVAEVVRRQSAMQRAVA"
     gene            complement(52335..54515)
                     /locus_tag="PLD05_00690"
     CDS             complement(52335..54515)
                     /locus_tag="PLD05_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOP75968.1"
                     /translation="MTSSTPRVRLVLLLHDHQPVGNFDHVFQQAYEDSYRPFLEVLKR
                     WEGIRVSLHTSGPLIEWLDSVHPGYLDEIAGLVNQGRIEIIGGPFYEPILAMIPSHDR
                     VGQIRHYTRWLENRLGAKVRGMWVPERVWEQSFTKDIVAAGIEYTVLDDYHFKAAGLR
                     KEQLYGYYLTEDEGRLLAVFPGSERLRYTIPFAEPHVTIEYLREIGEKHPNAVVVFGD
                     DGEKFGTWPDTKKHVYEDRWLERFFEALLREQDWLRVCTLGEAYDAVSPLGKIYLPDC
                     SYREMTEWALPTEQQRELESLRHEMSGDPRWSRVRAFIRGGYWRNFRVKYPEANEMYA
                     RMMEVSRRIQNAISSAPADSTLRDAQLELYRGQCNCPYWHGAFGGIYLPHLRNAVYEH
                     LIAADNLIEQHFRSQSAWVDVRAEDFDFDGQPEVRLANDHMVAYVAPFRGGQLYEWDV
                     RSIRRNLLATMTRRPEVYHDKVRAGASGHHEGCASIHDRVVFKQAGLESQIQYDRYPR
                     KSLIDMFYSPETTLEQVRSGQAVYWADLSNLQYETTIHANPDRVRVTLSREATVLDQT
                     VRVTKAILLSEGSPILEVAYRLENLPADRPLLFAVELNVAGLPANADDRFFESLEGAR
                     LGHLGTQLDLEETPGLALVDSWLGLRLQLAFSRPTNLWTFPVASVSQSEGGFELVHQS
                     VAIQPHWWVTPDATGRWSVMMRFLADTSAASQRESVAELATASV"
     gene            complement(54758..55783)
                     /gene="galT"
                     /locus_tag="PLD05_00695"
     CDS             complement(54758..55783)
                     /gene="galT"
                     /locus_tag="PLD05_00695"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOP75969.1"
                     /translation="MFSFSSKAERNAELRQDPITGKWVIISPQRAERPQEFDTRPRRI
                     RHGPCPFCPGNEELTPPATLIKPNVSASPQEPSWKVRVVPNKYPALVDSEDCAIESGI
                     YRGRTGLGVHEVLIETPRHVQGISDLTAEEMLDVVEVLMSRLAALKSKEHLRYVMVFK
                     NVGPLAGASVEHTHSQIIATPVIPPTIEAELAGTGEFFRGHQSCIYCQIVQYEIAKAV
                     RTVQITPHFLAMCPFAARFPYETWILPRQHNARFSDLERGLWGELIQITKDLIQRIEQ
                     TIPGVGYNVILHDAPLHDDVPFYHWHIEILPSTARPAGFEWGSGVHINSVFPEFAARS
                     LREGQAS"
     gene            56121..57167
                     /locus_tag="PLD05_00700"
     CDS             56121..57167
                     /locus_tag="PLD05_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOP75970.1"
                     /translation="MNSWAPFPFVLQTAIVLAASISTGHQLFGQEEPMVPPLQIYPQP
                     YSQAAFVFVPIPMELVRFHFGGTLFRPFLYPIRSPSGRELTRMGHPHDPISHSHHNSV
                     WMSHADVNNSDFWSDRTEARIVCRNVVEYGEQPGTPGQDGSFPTAWLLADIEWVSEKG
                     TPLLKEERRITLQLFESAEKSWRNSHSALAGGYLIIIDSSFRPATESPVTFGQTPFGL
                     IGVRMTKTIGVRDGGGRILNSEGGVNEKGTFRLPARWMDYSGPVTVDQKAGITLFDHP
                     NNPGFPNPFHVRDDGWMGICLTLNGPITLEKESILKLRYGLWCHDGVPGKEEIDNVFG
                     IYSRLALPGQQRQQ"
     gene            57306..58700
                     /locus_tag="PLD05_00705"
     CDS             57306..58700
                     /locus_tag="PLD05_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOP75971.1"
                     /translation="MDVRTLPDSERSPHAASEQSRDRRPKLMLFGLLFGLVSGLLVNI
                     AIEYAAQHPNETFSQWLPVTAIQWSIDNVIEPVGRVFLRLILSVVLPLVFSALVLAVV
                     EFRDLRRLGRLGLAAIVMTVIFSAASVLIGLTLVTVAAPGKAMSVEKQQAIEARFGRR
                     ELPREDQVKRVPDIVLDLIPENPLQEAVGALDGSSKGNGMLAIMVFALVLGAATIKAE
                     TRGEPWIKVLDSLYAVSMIIVDWAMTLAPFGVACLMFVAGARLGIALVVPLLGFLLVV
                     VGGLLLQMFVVYPAALWISGVRPSQFFRNLGPVIPTAFGTASSSVTLPTAIQVAERNL
                     LIPSHIARFILTVGATGNQNGTALYEGVVVLFLAQVFGADLTIWQQLGVVLFAILAGI
                     GTAGIPGGSIPMIMIILASIGVPPESVALILGLDRFLDMCRTVVNVTGDLTIAQCVAA
                     WSGHLPKGKHDGTT"
     gene            complement(58775..>59866)
                     /locus_tag="PLD05_00710"
     CDS             complement(58775..>59866)
                     /locus_tag="PLD05_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP75972.1"
                     /translation="YYDGGNRFDLKKWNPEYFSRLHAFMQAARDRGIVVEFNLFCPFY
                     NDELWAANPMNVKNNVNGIGACPREEVYTLKHADLTEVQLDFVRKVVSELREYDNLYY
                     EVCNEPYFGGVTEAWQWKIADTIVETEKTWGVRHLISMNIANGRAKVEKLHPAVKILN
                     FHYCVPPDTVALNYHWNVVIGENETGFRGKDDILYRSEGWDFILAGGALYNNLDYSFT
                     PAYPDGTFLDYKSPGGGSATLRRQLSVLKKFVEALPFWQMKPDSGTIKGVEPDLTASC
                     LSKKGAVYAIYLHVPLPNKPKNLAEHLRKNVTAKVALDLPTGRWRVRWLDTKSGAWVL
                     DESRQHDGGLLTLTSPAFDDDIALRIDRD"
BASE COUNT        13859 a        16535 c        15845 g        13227 t
ORIGIN      
        1 gtccatgagg gacgtggtat tgaacgacaa ggcaggacga tgatcgtcaa ctgcggccat
       61 gccgcctccg gttattacgc caaaattgtg atcccttccc gcgactcgga aaatcaaatc
      121 caagtcgagc tttgccgcgc taaaaccccg tgaaatcggg atcggaaacg aatcttccga
      181 ttcaggtcgc ttcccctttg gattcgtccc tctgagcagg tcttcccagc cgagaagcag
      241 gcgcttggtc ggaccaccag gacgagggca ctaagtacca cacgacaagg cagctcacac
      301 accaacctaa tccggccccg aatagcacat cgcttaaaaa atgggccgaa ctcacgatcc
      361 gttgggcgca cgacaagacc agcaataccg tgaagattgg tttcgctttc ggataaagaa
      421 ggatgagcac aaatgccaag cctgccgcag tagcagcatg agatgaggga aatgagctac
      481 ttaagtgcgt gaactgacgg gtagccacgg gtagccactc ttgaaaggtg gtgagaatcc
      541 ctccggcgaa atcaaaatcc cggggacgca cgcggaaaat gagcagcttc attaagtccg
      601 cggtcaagcc tgctgaaaag gccccgatca ggaccgtcat cccccggcgc catcctttgg
      661 tgtcaagcaa acccacgaga aaaacgatac aaaggacgcc aagtccatgg gcaaaaaact
      721 cgctgagatt gctcgctttc accaatagct tggggtaggc atcctccttt gcccaaacgg
      781 ccaacgaaag atcaacagcg aaggccgcgg ggcttagggc catcagtgcc aagcctatta
      841 gaagactgcg atccacttta aaagtcaatc tcaaccggcc ctccgcgtgt ttgattgctt
      901 ctgttacgag aactcgctca cctttggcct attctaacca aaccgcggat gacaaagaga
      961 catcactttg gaacggccat tcgtgtttca tcaatcctat cgttaccttg cgagtgacgt
     1021 gccctcgcag gtggattgtc taggttggcg tacaccgtag aatggcgagt aacagttttt
     1081 taggtaagac gggtccagac cgctatgtta cttaaagatc aagacggtat ctctcatgtc
     1141 gatagcgttg tcgtcgctcc ccaccctgat gatgccgaac tgggaatggg aggaaccatt
     1201 ctcaaaatgg tggcgtccgg cctgaccgtt ggcattgtcg atgtaacgaa cggtgagccc
     1261 accccctatg gaacccccga aatcagggca gccgaaacag agcaagcgag tcgcgttctg
     1321 gggattcgat ggcgcatcaa tttgggaatg ccgaaccgca gcctgcagcc aaccctggaa
     1381 gggcgggccg cgttggcagc ggtctttcga ttaagccgcc ccaaggccgt gttctgtcct
     1441 tattgggagg atgcacatcc ggatcacctg gcaactaccg agatgaccga ggccgcccga
     1501 ttttgggcaa aactaaccaa gaccgatctt cccggagacc cctactatcc tccccgaatg
     1561 tactactact tttgcacgca tctccgcaaa gtgtttgtcc cgagcttcgc cgtcgatatc
     1621 acgccgttca ttgataaaaa gctagaagcc gtgcgttgtt atgagagcca agtgattatg
     1681 ggccgtccta cggacccgta ctcgtttgtg gactcgctta ggcaccaagc tgccgcactc
     1741 gggacagcgg caggcgtggg gtttgccgaa ggcttcgctt gtcgggaaac cctcttggtc
     1801 gatgacctgc gagctttgtt tcctgttcca agatgagggc ggttgaagtt gcgctccagc
     1861 tcggagctgt tctcacgcgt ggactccccc ttggtaacta caccgcgggc taccgtgctg
     1921 acggctcctg tatcacaagc gcatcacaaa attgctattg ctggtttttg aactgtttgg
     1981 tacaaattcg tgatttgtag tagagattgt ttctcagcgg gcaaccccgt cattaggata
     2041 gctgtgaccc atatcttctg cgaagtgaaa cgatacggcc ttaattcggt gaaaaacggc
     2101 taacggctaa gaaagtcacc ccctccctcc gggagagggg tggggtgagg gcaagcgaac
     2161 atcggaatcg attgagaaaa ccggcagcga ttcgagccac cctcaccccc gacccctctc
     2221 ccaccgaacg ggcgagggga gattatggga taggctctaa gcacgaaact cttcaggaag
     2281 cagtgagaac agtcatggga aggcgacatc acccccatgg gtcccccacg gatgaggcca
     2341 ccgatatgaa ccagagccca ccggaagacc aaaccactgc ggcatctgcc gaagaacttg
     2401 gggaagacat ggagagttct tcggcactca ttagccgcta cagttttgag attcgtgtgg
     2461 gactagccat ccttggagta ctcggtttta tctttgccgc tgtcgtggct tatcggatga
     2521 tcaagctgcg acaggatgaa gtgaatcgga caaagcccac cgaaaccgct caagccggcg
     2581 atccgaccga atcgcaaacc tcgggccagt cccactcatc ggcccaagag gaggcgtctg
     2641 gcgaaagtat cgctccccca ccaccttttg ctctagattt tcccaacagt ttcccgtctt
     2701 ccgcacaatc ggcaagcagc ggagcgacag aggcgagtca tcctgtgcgg aaccagcggg
     2761 ccgcgcaagg gtgggtcctc ccgcctgatg gcccggctga agaatcaccg ttaggtgcag
     2821 ctacttcgac gaacgatccg gccacagggt atgcagcaat aggcgacagt gcgggaacgg
     2881 catcttcatg tgctcaactg actcccgccg cgcccgtagc agggaatacc ccgcctgggt
     2941 tcagcaacga ctcatccccc ctgtacggcg ctgggcaaaa caacagctcc gcacctattc
     3001 acgccagtga cgcccgtgtt cctgatttct ttgcagaaaa tcgctctata cagccaagct
     3061 cgctcactcc gggcccacca atcgacggtt ctccgaatca gcccggtcct ttcgcaggga
     3121 gcacatctcg gcaaaatctc gaggctgaat caggaatacc tttccccggg gactttttcg
     3181 ccgggggggc cgctctcagc tcccaaggga atcttggtgg cttaccctct tcggtacagc
     3241 cagccgggca ggacccccag cccacctcga atgacaaccg ctttgcgaca aacaacgtga
     3301 cgccaacggt cgatcccttc tcccgctcgt ctggagtcgt caacccgagg tcgcaagaag
     3361 cgtcagccgg ggtaccccag gggggcgtgg cgaccactca gggaccctca ccatattcgc
     3421 cacctcttgt cgccagtgag tccaatgtcg gggaaaaccc ggatccgatg gcgggcaatt
     3481 acatcacgca taccgttcag gagggtgaaa cccttttcga cattgcccgg caaaggctag
     3541 gtaaggcgtc gcgttgggtt gatatttacc aactcaatcg aggccaattg ggagagcgac
     3601 tcgagtattt ccgccccggt ctaatactcc ggttgccagc agatgcaacc cattcgaccg
     3661 caacctcgcc aacaacgctt cgctagaagg ttttcccgaa ggccgatcaa ggccaagggg
     3721 acgtgcgggt cactccggtc ggtcggtcag taccacccga aggtcgcaga cgttggtatg
     3781 agtagggccg gtgcgaatga gtccaccaag cggttcaaag aagtgaaagg cgtcgtttcg
     3841 ctctaaatag aaacgtggtt gcataccccg ctctttcgcc gctttgatta ttgcggcatc
     3901 gacaaaggcc ccggctgcgt ccgtggggcc atcctctcca tcagttccgg ccgagaggag
     3961 gatcaaacct tctggcgcgt cattccaaag catctcggct gctgccagaa ccagttgttg
     4021 attacgacct ccccgcccgc gtatttcagg aggtgcgagt ctcacgaccg gctcaccgcc
     4081 gctgattagg caatgacgac ccggtcgcct catctgctca agcgcctctc ggacgaggaa
     4141 ccttcccaca tcctccgcaa ggccttccag cgttgcggcg gtcctcgagg ttaccatgta
     4201 acccaactca acggccttgc gaccggccgc ctccacagcc gtttgattat ttccaataac
     4261 aaggtgaaac gcttgagtgg tgatcgaatc cgaagtttcc gacggctgat tggccgcgcg
     4321 cttctccaga acatggaaga tgtgttgggg aatttgccct tgttggccgc agtaccggtg
     4381 caaaacatct aatgcctctc ccggtgtagt cgggtcaggc acggtaggcc cggaggcgat
     4441 cacttccagc ggatcaccca acacgtcgga gataatcagc gtaatgagcc agcccgcccg
     4501 gcactgccgc aacagtttgc cccccttaac cttactaagc tgttttcgga ccgtgttcaa
     4561 ctcggcgata ttggccccgg cagcactcaa cacctgggta accttttgct tatcttggag
     4621 tgtgattccc tcaatcgggg ctggaagtaa agccgatcct cctcccgata gaaggcaaag
     4681 gcaaacatcc ttgggcccag cagaacggac gagttccaag attctttccg ttccataaat
     4741 ccccgactca gtcggctcat ttacgccggc cggtcttgcc ggatgaaggc gtatccagcg
     4801 cgtaggcccg acacagtctt cgggaacgtt cacccagccc gtcagttgtt tttccgccgc
     4861 caggttgtcg ggaatcgctt tttcgaggcc tcgcaccata cctgccccgg ccttcccgac
     4921 gccaatgact agcagccgat cgacacgatc aagctcgatg actagatcgg cgttttttcc
     4981 gatcagcagg cgacttcctt cgacacaaat gttctcggca accaggcggt ctgccttgac
     5041 agcatcgagg ccagcgtgcc agatggccag ggcgtcattt cgcaacgcgg atgaaaacat
     5101 cgatcaatcc atctcttgat cacttcaatg tgagcgtcac catccgcaga tcaatggcgc
     5161 gttcaccagg gattttcgtg gcgcggatcg agagttgacc gcgctcggct ggtaatgtaa
     5221 gagttccaaa taataagggc ttgaagtcct tcacataaga ctctccctca cgcggcacgc
     5281 ggtcaaactc ctggccacgg agaggtggat cgaaggcctc ggtgacctgc cccttaacgc
     5341 tagccgaacc aaaactaacc tcaagttcca cccccacatc tttctcgcga caggtgtaga
     5401 agacacacac ctcgtaggtt ccggccgtgt gaacctcaat atcccaaatg attgcatcgt
     5461 caactgacag ccagttcgtg aaaaacgaac aattaggcgc gttcgcacta cggcggatat
     5521 ttccatgagg tacgccgtcg cgggctggca aaaacgttac gggaaactct cgatatccca
     5581 ccgggaaggg acgatcatcc ggggtctgag gcagggcagt tgcccgaaac tcgttgacag
     5641 cagcttgtag tttctccgcg acgtccggta gctccttggt gatatcgcgc gtttggcccg
     5701 ggtcagtttg caaatcgtat aggcggccct gggcatctaa catgtaccgt tgattccgaa
     5761 cactgacccg acctgcctgg tgactgaaaa tgagacggtc cggccaagcg gtctgcttac
     5821 ccaataggag cggcgacaag tcccatccat ccagcggctt tagaggattc gccttgattc
     5881 cggccaagga gactaatgtc gggaataggt cgatcgctgc ggcaatttgg gaaattcgaa
     5941 gcccagccgg gatatgtttc ggccaacgga taacacacgg tacccgaacg ccgccttcgt
     6001 ccgtcgagcc ttttcgaccc cgcattccac cgttccatcg ccaactnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnaaat caatcgcatg atcggtaaga tcgtcggtga
     6181 tgtaaccctt tcccgtgacg atcttcccat tgtgttctag cggcggatca aaatagatcc
     6241 cccaatgccc ggaggtgaag ccataaaact cctgaaatcc ccgcgccagc ggatgataag
     6301 ggtattgact tccgttgtgc catttcccaa agatcgccgt tctgtaattg gccgcccgaa
     6361 aggcctccgc cacggtgtac tcatccaaat tgagccgttc tgctcccgtg gtcaccccgt
     6421 gaacgcccgt ccgcgggtga taacgccccg tgagaaactc cgctcgcgtt ggcgcgcaga
     6481 ggggactgac atagaaacgc tcgaacatgg ccccctcgtg ggctaagcgg tcaatattag
     6541 gcgtctgtaa atttggattt ccgtggagac tgagatcacc ccatccctga tcatcgctca
     6601 gaatcacgac gatgtttggt tgctttgcag gagtggcttc tccgtaaaaa ccccaaagaa
     6661 gcaagaccat cccacaaagg agcaatcgca aacaagcgac ttgcagacac cacattgctg
     6721 gcctcccgcg caaagcggct atagagaact tcgttcctac gcgtgacgat cattggagga
     6781 cacgattgta attgaaatag aatcgctttt ctcggtggga tgtcaagtgc cgccactcgt
     6841 tcactggatc cggggatcca acaaggtcaa ccaccagatg ccgaacatgg attcgctgat
     6901 ttttcagcac gcgccaatcg aacgggtact ccgggcgaaa tacccataat ccccactgcc
     6961 gcctggggat cattcttaga tcacgtcgat cggtaaagac atgccccagc aactcttccg
     7021 gcaactcggc gcgggaaatg gccagaggtg gacgtcccat cacatagagc gataacccca
     7081 cggaggcatt agcgcagagg tcctcgattt gacggcggtc agcctccact gatacagcaa
     7141 cacaccgtgc tccaagtttc cgaagataag cagcagccaa gggattcaac acacctaagc
     7201 ccgggcctgt ctcgaatgcg acacccgccc ggcgtgccaa cagccatcca ccccaggtat
     7261 tgacttcgac acccagccga gccttcttac atcgattgat ggtttcctca atccgaggga
     7321 tgtctcgctc aaagaaaaca ggcggcaagg cgataacagc cgggactttt cggcacattt
     7381 gtgcgaccgc cgagacgtcc tcggggttaa catcttcgac aatcacatgg accttggggt
     7441 cctcccggag aaaggcttct aactgcaaga atgagagccg gacgcgattc ggttcatcac
     7501 ccaaggtgcg attgtttgcg gtggccggac ataaatcatc ggcggagaca cgggcccctt
     7561 ctggaatctt gatatccaag gggcgattct tcgcccgctg aatccgagcc atttccgcgg
     7621 tgatacgatc gaggattgca ttcgcagtgc ggggaacaat gagaaaatcg ggatcatccg
     7681 tctccgctgc atccagaaaa atcccctgaa atcggcggcc ttcgatctgc tgacacacct
     7741 cttgagccga gagtgccttg tgcacgcggc ggaccaccgt cttgggatac tcccacgata
     7801 tgtccttacc ctccacctct aaattgcagc gaatccgggg gccggtcact tcgaggtgaa
     7861 acaccacgga accagcggat gaatcaaaaa cttccccatt ctcgctacag ctcacgtcgg
     7921 tggtcgaatc ggccagatcc ccgtcctcct caattagcga tgtctcatcc cgacctaccc
     7981 ggcctcgatt tgtcccagtg agattcgtac ggcgcccgag aaggtaccca tccgtaaggt
     8041 cgcggccagc atactccgtc aacgctttag cgagagacag tagtgagttc ttgtcggctg
     8101 tatcatcaag ggcctgccgg tacaaactta cggccctttt tacccactgg gccgacttga
     8161 gtcgtccctc aattttgagc gccgaaacac cagccgcaga gagttctgac aagtgttcta
     8221 aaatacacaa gtcgtgcatt gaaagtggaa aaccggccgg ttcgccatcc acttgccagg
     8281 gaacacgaca tgggctggca caaacccccc ggttcccact acgcccaccg acccaactgg
     8341 acatcaaaca ccgcccggaa atagaaaagc acaatgcccc gtgggcaaac acctcgacgg
     8401 caatccccgg caccgaacaa gccgcccgga tttcatccaa atcaagctca cgggccaaaa
     8461 ccacgcggtc ggcaccaagt tcgcgtgctg cttcaacatc aacactgttg gcaatggtcg
     8521 tttgcgtgct gaaatgaaac tccaacttcg gatacgcagg ccgcagagcc aacaaggccg
     8581 gatcacgcac gagcaccgcg tcgaagccga tctcgttggc ttgctccaag gtgctcagcg
     8641 ccaatccgag ttcccgataa ctaatatccg tattaagggt gagaaatgcc tttcggccat
     8701 gggagtgcaa gaattgcacc gctcgggcag cctcgtcggg tgtgaaattc ctggcgcgcc
     8761 gccgggcatt gaggtgtggc aacccaaaat agacggcatc cgcgcccgct tcgacagccg
     8821 cctccagaca cgcgaaatca ttcgcaggtg cgaggatttc cattaaaccg ctcagcaatc
     8881 tttggcttca ccgaaaatag aagcagacga cagcgcccct ttaagttatc ttattcttac
     8941 ccggaacacc tggacatatt cattccagct cgacagacga ccgcatttta agtcgtgacc
     9001 ccgaaaatcc tgttaaggat gttcaccaac ggcttcacaa cgagattccc accagaagtc
     9061 tgccccgggg gtggcggttg cttcccaacc tgatattccg cgccgagctc tcgtgctttt
     9121 acaaagttgt gatccgcatc gtgctgccca atcggacaga gcatgacata gggcagcttc
     9181 tccgccttca gagcctcttt gagctgctta aagaattccc agcccttcat atcgggaagc
     9241 ctatgggcac ccaaaatgac gtcgtatccc ccctcacctg ctgtcatcac ggccatctgg
     9301 attcccgcac gtcctgtgga agcggtttcc accacacatc cccattgttc taaatactta
     9361 tgggcatccc tttggatcga ctcgtcagga tccacaacca aaacgcgttt gttttgaaaa
     9421 ataggcgagg cctcagtcgg tggtaatcga gccccaaacg agcggatctc cgttcgcaaa
     9481 tcgcgagcaa tcttgtggat ttctttgaga atcgccctgg ccttttccac gtcacagcgt
     9541 gccggatcat gatcgagata agcatcggct tccgccgctt tgctaacgat ttggtccacg
     9601 ggcagagcaa cccttccatg gatcgcctcg acaaggcccc gtgtcgccac atttgcctcg
     9661 gcttcgatca agtccaacgt ggtcagtgca accacaagat cgcggcagaa gatttccaga
     9721 aacattcgat catcatggga gaatgcgcag ggccgcggac tttcgatatt cactgtgcca
     9781 atcaccacag cccgcgaagg cccttcgtga cggatcagag gaaccgtcag agagctccgc
     9841 gactcctccg tccctttcag atagagggca tcgctggcgg tatcctcaca gatgtaactc
     9901 gccccggttg ccgctacata tccagtaatt ccttggccct gaagaccaac gaagagccgt
     9961 cgctgggccg cctccggact cattccgtcg gagataaagg ggaccaactc cttggtttgg
    10021 gggtcaatca atcggatttc aaaaacgtcg aagtgcaaca ccttcttgag ataatccagg
    10081 acccgccttt taaggacttc ttttcgctcc tccgcgctgt atcttgtaac aaattcgggc
    10141 gcgaattccg tcaggtgccg tcctgcctca tgaattgccc gcaacctctc gagagggaga
    10201 aaggttgacg ttatctcttg catacttacg atgagacgga ctcgatcctc ctccgtctgg
    10261 ggtatccgtg acgcaaacag ctcaaaaaac cgcgggcctt tgagacgcat aacggatcgc
    10321 gccgggcgtc ctgtatcacg tgcaagcgtc aggggaaaga actctggccg gatgagctgc
    10381 acatcccgga acaaatcctg ataaaattgc ttcccaacta atggagtcgc cgcaggcagc
    10441 agttccgatg ccctgttgtt cgcccaaacc acccgaaact tttcgtcaac aataaagacg
    10501 gcctggggaa gatatgtggc gaccgcggag gtaagattat ccacaggaac agtcccctcg
    10561 tcttttgctt cctgtaaagc taagccgact tcatgatccg ccttttcaag agtaggatac
    10621 atgattgttt agtcctgtca tcgggcgcaa cagtgggcca tcacccgctg attcgcaacg
    10681 ccatagcgat gtaaccacta agagggcaac tcgctgcatc acgatagccg ctaactcccc
    10741 aacatattgt aggcgccgcg ccacggttgt caactactgt ggcaaacgat tgcgatgcgg
    10801 accacatggc cgcggcgttc cgccctgcca caatctggtg taggcgcaat gaacgtaagt
    10861 gtagaaatta aaaacacttg attcgtttgt atccaaacga gctcgccaag aatcttctcc
    10921 accaatcaac gggcgaaacg gtttcgtgac cacactagtg agccggcgtt tcaaactgtg
    10981 gcacagcacg gcgtgacgca cggcggtggt ttctttgttg caatttgccc aactattcca
    11041 acccatctct tgtgatccgc ttttatctgg ctgctatcac cgagacgatt tcaatcattg
    11101 aggatcattg ttagttcccg cgatcgtgat tttgttgggc acgcgccaga tgagccgcgc
    11161 ttttttcctg gccattgggc tgtttgtggt cttcttgggg gttcaaaccc tcggggtcca
    11221 gcgatttgtg ctgaaaacgc gggatccagc cccccctggt aatgtggcca taggcgaaag
    11281 ccaacctggc cccaataagg tcctcgaacc acccccctgg gcaccgtgga gcttaatggc
    11341 aaccggggct atcgtctcgc tttacgccgt gacactcccc accgtgcgta aataagcccc
    11401 ttaattcgcc ctcaccacaa gagcgggttg tccgtttatg ccggtttacc gagaaaagct
    11461 tcgctacaca acacaggggc acggtcatat gcttgacctg acccaggacg tggccaaagt
    11521 ctgcaatcga gcaggagttt ccgacggctt ggtcaatctt ttcgtagtgg gtagcaccgc
    11581 agccatcgga acgatcgaat ttgaacccgg cttactcaaa gattttccca atttgcttga
    11641 caaactagct ccacctggtc gcaattatgc ccacgaggcg acctggcacg acggtaatgg
    11701 ccattcgcat ttgcaggcca cgctacttgg cccctctctc actattccac tggagtccgg
    11761 ggctctggcc ctcggtcagt ggcaacaggt gtttctgatc gagtgcgatg tgcggtcccg
    11821 gcagcgcacg gtcatcgtca gcgtgctgag ctaactccct ctcttcgggg gccatccgga
    11881 ttttcaaaac agttcatcat ttcgaggacc gatcccgcag acgttttgcg taagcgggcg
    11941 gaatcatttc cttgagaagg ggggataaaa gcttttttgt ttcttttttc cggcaaccag
    12001 gtataatatg atagcagtgc attcagttgg atttgggggc gacggaaggg catatgctgg
    12061 ttctatcgcg gaaatgtaat gagcagatcg tgatcggcga cgacatcatc gtgaccgtcg
    12121 tgtcgatccg gggggggact gtgcgcctcg gcattgaagc accgccacac gtgaccgttc
    12181 accggctcga ggtccttcag gcgattcgtg cggacgaggc tgcgcgaatg aaagatagca
    12241 gcaacaattc ttcccctccc accaccccgt gagctgtctc gtgaggtttt cggacggctc
    12301 ggcaggtggt agcatttctt gtctttatgc cgcttgattg gcattgggca ccgcaatcgc
    12361 ggcacaggtg catttgccac tagtttggga tagctttcac cagtcgactt atctacgcac
    12421 ctcggttaga attggcctta tccccgttca aataatcgga aatcccaata tgccgagcgt
    12481 aacgctcaaa cacatctgag ccaaacggag ccaaagatca ccgatgagtc ctgtggttac
    12541 agcaacagcc attccaggct tgcaaaaacg gagtggtaag gtccgtgata tttatgatct
    12601 tggcgaacaa ctcctcttgg tcagcactga tcgaatctct gcttttgatt gggtcctgcc
    12661 taacgggatc ccgaacaaag gccaggtgct taatctgctt tcggcctttt ggtttggcat
    12721 tttgggcgaa cctaatcatt tgataaccgc tgacgttgat tcgatgcccc ttcctcccgg
    12781 ggctgacaag gagacactgc ggggtcggac gagccttgtc aaaaagacgc gcgttgttcc
    12841 tattgaatgt gtcgtgcgcg gctannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnngaggta ctgacgcccg acagttcgcg gttttggcct gctgatacct acgaaccggg
    13021 caagccccaa ccctcgttcg acaagcaatt cgtccgggat tggttagaaa gcaccaaatg
    13081 ggacaagaac agcccaccac cggctcttcc cgaggatatt gtgatcaaga cgcgacaaaa
    13141 atacattgag gcttacgaac gtctgacggg aacagctttt gtcgtgtgag agagtccata
    13201 gacagaagct tttgtgattg gggatgtcgc aatgccgttg tatgaaattg aaaccgagta
    13261 tcacattatc atcacatggg ctgataacga agacgaggcc aagctcgtcg tgcgtgacag
    13321 ttatccacga gaagcgattt taaggatcac gcggcgaccg cgaaacagtt gggtcatctc
    13381 caaggccgcg ctagggatga cgggtcgtac cgatccctgc aacatcgcac gagattgcct
    13441 cgccaaagcg gcaggtgaca agatccatgc cattcgcctt tacatgcagg aaacgggtgt
    13501 tgacctggaa aaagcccgca aggttatcga atcgaatatg attatggggt ggtgacagtc
    13561 acctaaacac agtctggtga tactcaccga atgcgtgtcg tttgctgaag attgggctgc
    13621 aggtcgtgtt ggttcctcat ttacgttgga tcacggcggc caggttggct ttgcagcgga
    13681 tttttcgctg tcggcggttt ttaaccgata cgctcgccaa ccatcggtca tgacgtccca
    13741 cagccctgtt tcggtatcag gcgaggcacc tcggaagatt tcagacccaa attgcgtcga
    13801 aacataggat cttttttgct cagctcgcaa taacggggac tgtcttttca cgccctgtct
    13861 tcctgtctca aaatgggggt atcatgggca agttgggcgg ttggtctgcc gccacttttg
    13921 ttgaaaccag gggaattaag aatgcattgc cgtaggcatt ttttgaaggt gtgtagccga
    13981 aataggcgaa ctttaccggg gaagctcaat tcgctcttgg ttcccttcct aattgggttt
    14041 ctcatgacat ggggggcggg acaaagtttt gccgaaagcc catggattgt tccaaaccaa
    14101 gttccatcgc ctccgagtgc cagcccccaa gctgaatcgc cgcaacctct gccggtaaat
    14161 ccggttcccc ccgctcaaca ggggacccta ggggaaagca aggcatcggg aaaggagcag
    14221 actccctcca acctccgagc agcgttggac acgcggatta aaaaggcctt gcagcgtgtg
    14281 tgcccctctg acgcaagacc tgtgggcctg ctaactatcg ccgaaaggaa ttcctctgca
    14341 gcagctcgcc aaaagggcgt cctgtcattt tgggaagtcg cgcgggtcgc catggctatc
    14401 gatttcctga ccgagtgcct gtcggagcta gaaaagacac aagtggcttt aggcgaccag
    14461 atggcacttg aggccgtcaa gcagctttat acagcccgca tttacgaatt gcggggcagg
    14521 ctccaggctt caatggcatc cttgttggag gcagtaggca gcgatcgtga tcgccagccc
    14581 gtgtttcccg acgacatccc tcacaccggc ggctatcgta cacaattgga gaagctcttt
    14641 ccagaaaccc caccgctggg cctgcggcgc ctcaatgaaa tgattcccct cgctcggcaa
    14701 tcaatctatt tgcactttca agccagtcag agtgcttacg atgcctggct tgcctgcaaa
    14761 gaagcttatg acgcacagcg gggggattta tggagtgccc tcgcctgttg gcgtctttgg
    14821 gtcgatgaac aacttcactg cgcggaagcc atcgagaact acaaccgact gattctggaa
    14881 tatgtcatca cctcgggaaa cggacaacta tcggccgagg attttgtcaa catgctcgta
    14941 gaacaggatg ctcgccgggt ggtgtccggt caacgaacgt ccgcgaaccg tcctgatcag
    15001 atgccttcca agggtacatc agaagcgacg aaaacgccca gtgacccgca aactcgtcag
    15061 tttcctagct tttttcagca tgtgataaac gatcctgcta agccttctcc gccactgggt
    15121 gaggaatcgc cgtttctttt gatctggagc acttcgcgca aaccgtggcc actcattccg
    15181 gtatccttat cgaacgaaaa cggtccagcc gttgatgcgg gcctaagcac accctacttt
    15241 ggcaaattgc aacaaggctc cgctggagtt tttgtgcgac aagttgcgga actcagtttc
    15301 aagcgagggg tgacggccgc catgcaggat gggctgaata ccgatatcgc catggacgaa
    15361 aaactgctgt ccgggggaga tttcgcccac cgggctaggc tttactggga cgttgccgtc
    15421 gtccaggctg aataccacgt ttttctccag tattcagcaa ttctcagcga actctttcca
    15481 actgtcttgg cccgatggaa tgaacccggt gccgcagaag agatgttaca actccaggca
    15541 tttcgccgca cggtggatgc cggtcttcag gatatccacc tcaagatgct ggacagaggc
    15601 tccgacctga tcgcgcttaa ctcgaagtcc gacaatgccc atcccgtagc gattacactt
    15661 ccttacgttg gagaatacga taccagatgg gaccagattc ggacagtcct tccggaaccg
    15721 acccgcactg ccgccatgcg ggtggtttcc cgaatcccag ccacatacgc ggccttatgt
    15781 caaaacgctt tagcactgaa tgccgcagac caggcgcggg tttttgccgt aggacgtttt
    15841 ctcaacaagc aggagcgaat tggtcccgtc ttgagcgccc ttcacttcga gcacgaagcc
    15901 tggttaggct ttattgaact aacgggccaa tacaaccaac tcatcgccga gtatgtttcc
    15961 ctggtacgac ctgatttgga ccaaaagagt ttcctttctg ccatcgggat cacacaacgt
    16021 taaatccgcc gggtgttgcg cgaggtgact ctcgaatgca agagtctttc gcaaacaatc
    16081 ctcaatctga actgtctaca ggtgaatctc attctcctgc tggcgggaac gagtggcgtc
    16141 taacttcaag ggtaccgaca caggtgacga tcggccactt acgcatatgc ccacctctgt
    16201 ttcaggcccc gatggcggga tataccaact atgcatttcg ccaattacta cgttcacttg
    16261 gcggagtggg actgctctgc accgagatgg ttagtgcccg cggctttttg gagctggatc
    16321 gtcgctatca gcggctcccg gaccgattgt ggggtgtccg agatgaaccg cggccgattg
    16381 cggttcagat ctgggataac gatcctcata agctggccat ggtggccgag cgtctcgtcg
    16441 aggatttcga tgtatcgtgt ttggacatca attttggttg tcctgcggag caaatcaccc
    16501 ggggggtggc gtgtggggct tatctgcttc gtcagccaga cgacattggc gaaatcgtat
    16561 ccgccgtggt taaacgagta tcccctgtgc cggtcacggc caagattcga cttggttgga
    16621 gcgatcaaga gatcaacgca cccgcggtcg ccgaagttat ccagcaagcc ggtggcgccg
    16681 ccgtgtttgt tcatggtcgc acggcacaac aaagataccg cggaactgcg gattggtcac
    16741 agatcgctgc cgtcaaaaag cgtgtccagc ggattcctgt tatcggtaat ggcgacattc
    16801 gttccgccca acaagcggta acggccatga gggaattcgg tgttgatggc gtcatgatcg
    16861 gtcgtgtggc tttggcacgt ccctggatct ttcgcgaagc cgccgccctt ttggcaggct
    16921 tggaaccacc gcccgagcca cctgccgaag aacagtacga aatgttactg gaactttacc
    16981 gccagctcga aagctatttc gggagcgtga aggcaggaat cctcatgcgg agctttgcca
    17041 gtcgctttgt tacagcgcga cgtggtgcta aacggtttcg ggtacttatt catagctcaa
    17101 agtcatcagc agacctgctt gcccacttgc gggatttctt tttgcacaat cctgaaagct
    17161 aggacgtatc ccagcaataa cggaactgcc aatcgcatcc cgaagaatcc ctcttggcta
    17221 tcaaccgggg ctggtgttga aagagtccaa gacccatttc cgtgttcggc cttgagcgtc
    17281 agcttgccaa acctgtcccg actccacatc aagggctgtg agccacccac cgccgtagca
    17341 gcatgtatca atacacacga gataaccgag gtcaaggatt tcgaagttcc gctgggccgt
    17401 atgtccaaca atggcctttt ttcccgatcg atgaggcccc ggaagccgtt gccgcaaaga
    17461 ttcccaacga agtgtgaata cgtcctgttc acttagcggt cgattttcaa tgtagtttgc
    17521 gtggagaaag atgtgggtgg gtgtttctaa gtaggtgcgg cattcctgca aaaaacgaat
    17581 gtgatgttcg ggaacaccat ctaatgttgt caaaccgtaa gattcgagcg tcgccgcacc
    17641 tccgtaatgc aaccagtcat caaaaaggtg atgatctcct tgccaaatgg aaagcatcat
    17701 ctcgtcatgg ttacctagaa tgggaacaag atgaacttgg ccctgcaact caatgagaat
    17761 ttcaagaacc tcccggctgt ttggaccacg atcgacataa tcgcccagcg tgatgaccgt
    17821 atcactacct gaaggaccaa tctgatccaa cactgcacga agtgcgacgt cgtacccgtg
    17881 gatatcgcct atggctatag tgagtccact cattgtcgtt tctgtgtgat ttctaatgcc
    17941 tctgccgtaa ggaatgagca ccccaagagg ttaccaagag accgaggcct atgcggatgc
    18001 tgccggttgc aatgccagat aaatctgcgt gaggtggaag aagaccggag cggcaaaaca
    18061 gagcgagtca attcggtcaa ggacccctgt atgtccggcc acgagagtcc catagtcctt
    18121 aacaccacga tcccgtttga tcgcggacat cgtaataccg ccggcaaagg ccactaagga
    18181 gattaccaag gacagtccgg cggtaatcca gattgaatta aagggcaagg cccaccacag
    18241 catggcgccg attagggtta cactagccga tcctcccaaa agtccctccc acgtccgcgt
    18301 gggattgatg ttcggcacca ccacatgtcg gctaggaagt tgggaccagg caaactgaag
    18361 ggcatcgctc agctgggtaa taaacactaa aaaaaagagg agtcgaaggt tgtaggcatc
    18421 gtttccgagt tccaatgtgg tgagaagggc tggggcatag cttaagcagt acacgcaaat
    18481 gagaaggccc atctgaatct ttgccgtccg ttccaaaaat cggacaggat cgttggagaa
    18541 tgcaatgcgc gtatggagaa agagtaatgc gtaaacgggg atgataatgg caaacaaatc
    18601 gtattgcgcg taccccacca gaacatactg ggcgggcgtg caaagaacaa agacccaaaa
    18661 aagcgctcga tggtcgctgg gccgcgttgg tgtcagggtg atgaattccc gcaaagccca
    18721 aaaggaaatg agcccaaaaa agacgacggt agcggtcttt ccgaggacaa tggccccagc
    18781 gagcccggca aacaacagcc accacgcccg cagtcgccct gaaaagcttt caacgatttc
    18841 tgaattgatg acttccggcc gactgcggcg aagaagttgg acgacaactg tggcaataat
    18901 aagtagaaca agaacggcgc caagaagtcc ccatgtgact gtgtcattca tggacgcaaa
    18961 cacctcgccc aaacgctagg gacgggtttt tccttgccta atgttctagg cctcgaggcc
    19021 aaaggtccct atcaccccca tgctgttatt ttttctcccc gacacccaaa ttgagaatct
    19081 gacggcccag ccgacttacc acttcgagtt tgatacccgc tttggaatca gccgctttga
    19141 tggcctcttg gaattcagcc aagttgttga ttacgcgatc attgaccttg acgatcacca
    19201 tgcccgtgga tagaccggcg cgggcaccag gcctccccgg atcggcgtga atgacaacca
    19261 caccatgggt gacgcggaca ccggtatccg gcgcaagagc aggatcgaaa tccatcagca
    19321 tcaggcccag ttctttatca taaaccctct tgcgtcgggg ttgatcctgc gactcatttt
    19381 gttgcttcaa tcgctctgcc aactctcgct ccgaaagaga ttcctgcaac tgaatctcca
    19441 gctcaatgga agttccatcg cgaaggacac gaaccttgtg gggcacgtcc agggaggcgc
    19501 gctccacaat ctcctgaaga ctggccacat cgcttacatc gacaccatcg aagctcaaga
    19561 tgacatcgtt ccgcctgagc cccgccctgt ccgctggtgt ctcgcgaatc acctgcctca
    19621 ccacgactcc ccccaaactc tggcgttctt ctccacttac cgccttgcga ggaagcaaca
    19681 catcctcaag cgttacaccc agatatgccc gccgaacacg accgtgagct aacagctcgg
    19741 aactgaccca cttgacaaga ttgatcggaa tagcaaaacc aacgccctga tacccaccac
    19801 tacgcgaaaa tattgccgtg ttgataccga cgacctcgcc atccaaattc accaggggcc
    19861 cacccgaatt gccgggatta atcgcggcgt cggtctgaat gaaacgaacg cgtccaattg
    19921 aacgcaacgt ccggcctttg gcgctgacaa tcccagcact gaccgtccct tccaattcaa
    19981 acggattacc aatagctatc acccagtcgc caatgtctaa cttgtcagaa tcgccaagtc
    20041 gcgctgccgg caaatcctcc gcggcctcaa tttttaggat cgcgagatcg ctcaactcat
    20101 ccgacttctt gtcaataacc gggaactcac ggccatcgac cagacgtacc gtgacaacat
    20161 caccaccttc gaccacgtgg cggttggtaa tgatgatacc cttggggtca atgatgacac
    20221 cggttcccat gcccaaatct gggtttcggg gaatctcatt ttgaaaaccc ggttccgagg
    20281 gtttcggaaa ttcctcccac ggcaaccgag gcccaccacg ccctgcctct tgtccctctt
    20341 tagacgtggc tgtcttgatc gtcacaaccg caggcagaac ttttcgggcg gcactacgaa
    20401 aggcaatcga taggtacttg gcgtgttgaa ccgcctcttc ctcggtctct cctaaaatgg
    20461 gctgccccag ccatggacac gtagccgccc aaaaaacaca gcaaatcagc cagaaatggc
    20521 gtcttggtcg ctgaacgaca gctgattcgc tcgcttggcg atgcacaaaa acggacataa
    20581 tccaatcctt ctgatgaaag tataaaaccg ttcgcttgcc gacttctttt ccttcgacac
    20641 ttttggccat cgaaaacccg cccattctgc aagtttatgc cattaacccg ggaagaatca
    20701 acgaagtacc tcttgatgag gtaagttttg gcggaataaa gcgcaagatt gatacgttgt
    20761 gcacgccggg aaggcaccac cccgaggggt cggggcgtct tgatagttag aaccgcgagt
    20821 tccgttcccg ctgccatcgt tgcacttctt caatgatgag gctacgatga atggctgaaa
    20881 tgctgggcta ttgggctcgg gagcaatttc aagcgagaac aacggcgaag gcacgggcaa
    20941 gtgcttcgct gtcgcggctg gtgtggtttg ttgcttgcag cgatcatgtt cggaggttac
    21001 accatgaaaa ctaattaccg gcgtcgtgca tttctgatgg gttcctcggc tttggcgatc
    21061 ggggccatga tcccatggcg ttttggcgtt ctgaacgccg cgaccgtcga gaaattggcc
    21121 gaaggtgccc ctcacgccga aaagctcgga tggcgattgg ggtgtcaagc ttacagcttt
    21181 aaccggttca cgttttacga agcagttgaa aagaccgcag ccgtggggct tcgctacatc
    21241 gaagcatatc ccggccagcg gctctctcaa gagaagccgg acgtgaaaac cgatcataac
    21301 ctgagtgtcc aggatcgttt ggaagttaag aagcgattgg ccgatgcagg tgtcacccta
    21361 gtgaattacg gagtggtgcg gctttccgcg gacgagggtg agaatcgaaa agtgttcgat
    21421 ttcgccaaag acatgggaat cgaaaccatt gtctcagagc caccgtttga tgccttcgat
    21481 gtcattgaaa agctttgcga agagtaccaa attaacgtgg cactccacaa ccatccgcaa
    21541 ccctcaatct attggaatcc cgataccgtg ctcaaggttt gtgagggtcg gtcaaaacgg
    21601 attggggcct gtgctgatac cggacactgg atgcgatcaa atatcaaccc gttggaagcc
    21661 atcaaaaagc tcgaagggcg aattatttcc ttccacttca aagatctgaa cgattacggg
    21721 cgtggcgccc acgacgtacc ctggggcaca ggcaaagcgg acgttcccgc cttgctccgt
    21781 gagatcaaac gacagggcat caaggccgtc ttctccattg agtacgagta caactgggaa
    21841 aactccctgc ccgagattgc ggaatgcgtc aagtttttcg acaaggttgc ggcggaactc
    21901 ctggcgcaat agggcggcga tcggttttga atagccaacc gttttgaacg gccttaatcc
    21961 cgtagttaca ccgacgagtc gaggcacccc attttggggt gtcttttttg tttgcttgtc
    22021 gtttctagga aaagttctgc gaagaaaaaa accaggtggc ctttagcagg acacgttggc
    22081 gtgcgccatc gccgtatgtg ctgtcagtgc aatcacttat agcaaccagc agtgcgcggg
    22141 caagccacat cgcaagaaaa aacaccacgc cacttgactc ctcggcccag cgttatcaca
    22201 gttttgcgat tgggaacgaa agtttgccac agttgttcat tgctgttcgc ataatgggtt
    22261 gtcaagtctt ttccggggac gcctagacga atccgttatc tcgtcaagaa taccccagaa
    22321 aacggtcggg aaatccccgg ttcggtcatc ggctgaattt cagaccctga gtttccggag
    22381 gtgtgagatg gatcgggaag aatcacaaag cacctttctg aggcattgcg cgctcgtaga
    22441 ggctactgcc gagcgacttg ccgccgaagg ggtaggatgg accaccttct ttcgggagct
    22501 gcttggaccg gaaggacttg ttcgacagct tttccccaca ccccagcaac ttagcgaatt
    22561 cgaacggaca gatacctacc aaaaggtcca ggaaatcctt gccaagctcc gccgactcca
    22621 ggaaggccgt tccgcaccgg acgaaccatt gactgtgatt accattaggg tcccgcggag
    22681 cctgcacgag gcactggcag aggaagctca cgctttgaag acaagtatca accgcctttg
    22741 tatttcaaaa ctggcccaat tgatcgacac gcgatttgtg ccgccaatgc gccacttgga
    22801 ccgagaggca tcccacgaag aacaagagct tacgcagccg ccttctcgta aaacacggaa
    22861 atgaaaaaat gaaagacgag gacaagggcg gacggtaagc cgggttctgt tcttcgagaa
    22921 actcgaagcg atggccattt atctaggagg ccgattgctc gacccctcaa gcggcctacc
    22981 cggaagtgat atagcggtcg ggatcacccg cagggcctca cttgcgcgag gcccactcct
    23041 tcctgctcgg ccttgctccc ggtggggttt gcctagccag gccggtcacc cgacctgctg
    23101 gtgagctctt acctcaccct ttcaccctta ccgcgcggaa acccgcgagg cggtctcgtt
    23161 tctgtggcac tttccctagc ctttcggccg gtgggtgtta cccaccaccg tgatcccgtg
    23221 gagcccggac tttcctctcg ccgtcacttc tgaaatgacg gccagcggcc atctcgtccg
    23281 ctctgtcctc gtccacatga atcatatgac caagacccga tggaagaaag gagtgtgcct
    23341 cgcttacgta cttccgcact tatttgggct cgaagaaagg ccgtccggct gagtcggccc
    23401 ctcgctgaac cggcatcctt gatatgtatt acggagttct agttgtttcc cgatgagcga
    23461 gatcgtttca cccttccgac gacaccagtc tggtgctatg agataacccg gatctgtctc
    23521 cgccgtcagt cgttcaatca cgatgtgcgg tggaagcctc tcgagggcat cgcataccat
    23581 gttcacatag acgtcttggg aaagcggctc atattggcca gcctcgtaaa gcttagccaa
    23641 tcctgtatct tttgcgacgt aaagattgtg tatttttacg ccgtggatcg gcagttccgc
    23701 aaccttttcg gccgtctcca gcatctgttg gtaagtctca cccggaaggc caaacatgag
    23761 gtgaacggta acacgaattc cctcattcgc acaacgctga atggcgttca cagcgtcttc
    23821 ccaggagtgg ccccgattaa tccgccgaag tgtagcatta tgcgaagatt gaaccccgat
    23881 ttccacggtt aaccaggttt cctgactgag tgttgcaagc agcttgacca cttcttccga
    23941 gaggcagtcg ggacgggtcc caatcgccaa accaaccacg tcaggcacgg agagcgcctc
    24001 ccggtatagc cgctcgagct tatcaggagg accgtaggtg ttcgttcccg gttgaaagta
    24061 cgcgatgatc ttctgaattc cacgtcgctc tcgcaggtga gtggcggctt gcagcagttg
    24121 ttccctgatg gttaggccag accgccgtcg gctcgggcta aagctgagga tattgcagaa
    24181 gaggcaacca gcgtgactga tggttccgtc cgcattgggg cagtggaaac cgccgtccac
    24241 gctgactttc caagtttttc ccccgaaaat acgattgaga tagaagctga gactataata
    24301 acggtagccg agctgccgcc aattataaga ggaggcggaa ttgattccga gtcttttgtc
    24361 cgggttcgtg cccatttgtt gacggcaaag ttaaccaagc ttacactgaa agacgacgtt
    24421 gtaaacactg tcatcctaag aggaagaata ttttgcgaca aggaactgag aacaggcaga
    24481 actcatgtac ggtgagctga tcccgctggg tgggggagac cccatacctc tcttgaagaa
    24541 acgtctcatt gtggggcggc gagaaagttg cgatatcgtc ctgcggttct ccaacgtgtc
    24601 cgcgcaacat tgcgaacttt tcgtaatgtc cggatactgg tacgtgcggg acctcaatag
    24661 cacgaatggt atcaaggtca acgggactcg ggttcgggag aaacgcctgg atcccggtgc
    24721 cgtattgtca atagcgaagc accaatacga aattcgatat aatccgattg agctgggcgc
    24781 cgtcggccct ccaccgccgg acacgctaga acaggacata ttcacacgtt ccctcctgga
    24841 aagggctggc ctcgtcaaat cgaagccggt cccggagcca gaggaaacag aaaagaccga
    24901 accgccctca ggggacgatg ggaaacggta cgacattttg gagggcttcc ggaagtccga
    24961 attcaaaaag tcctggacgg agcctcaatc accgaaatga caaggccaag aaacccacga
    25021 aacccgccga ataacaggga gagtttccgc atgctcctgc ccgcaagctt ttgccgggtg
    25081 ttgtgaatgg gaaaaaagaa aaaaatacgt gtcgatttca gaaaaaatcg gacttcaaaa
    25141 cctcgccaga aggatctcac tgatcgtgtt gtcagccgtg atgaggtcct tgacgaagat
    25201 ttgacacgcg aggagcggat cggcggacga ggggagttga cccgacgaag gaccgtcatc
    25261 ggccaggaga ccgatgataa atccggagtc gcgactcccc tgccggtgat tcttgagcca
    25321 cgtcacccgg atagtcgcct tggtcgcgtg attgccgttc agggtcttat atgccaggtt
    25381 gaggacgccg aagggcaggc ttatcgttgt gtaatccgtc gcctcttaaa acgcctggca
    25441 acggatcaaa ggaacgtggt cgtcgttggt gaccaagttc attttcaccc gttaccgggc
    25501 tcagatggcg aaggagtgat cgaaaccatt ctgccgcggc gcggcgccct tgcgcggact
    25561 tccaagggca agcaccatgt ccttgtggca aatgtcgatc aggcaatcat cgttagcagc
    25621 gctgcgatgc cgcggttgaa gccacatctt atcgaccgca tgattgtggc cgccgaaaaa
    25681 tctaggatcg agccggttat ctgcatcaat aagattgatc tggttgatcc agccgacttg
    25741 gaaccgctgg tcggacttta cagtcaattg ggataccgcg tgctgctggt cagtgcgatc
    25801 acggggtttg gtcttcctgc actgatccga acactcaagg acaaagttag tgtcctctcc
    25861 ggacaaagcg gggtgggaaa atcctcattg ctcaacgcga ttgatccatc gcttcgaatt
    25921 cctgtcgcgc cggtgagtga agacacggaa aaagggcgac acacaacgac gactgctcgg
    25981 ttgtatcggt tccatttcgg aggatacgtg gcagacactc ctggtatccg gcaatttcag
    26041 ttgtgggatt tgacacctga cgaggtggtc aattattttc gggacctaag accttatgtg
    26101 aatggatgcc ggtttcccaa ctgccttcat ttggaagagg cagattgcgc cgtgaaggac
    26161 gccgtggctg acggtttgat tgatgaacgc cgctacgaaa gttactgtca tttggtactg
    26221 gatgaagagg ttgaggagga agaagagtaa tcacgcagga gagtagtgtt caatgtcgtg
    26281 ttccgcttgg agtttttgac ggctagaaca aaactgtcac agccctatgc gtgggcagaa
    26341 catcgaaatc gcttcgagat cacacggata tttgggagga cgtgtacccc ggaaaggtgt
    26401 tcgtaacaac cgtaaatagc aatcggaggt gtgacgacat cgtgaggccc agaaatggca
    26461 gtcaaccgct tggtgaaaaa ccggagcgag gggtcctggt aggtgtgcaa cttccgacat
    26521 ccgtccttga agatccgcca ttggcggagc tagccggctt ggcaaaggcc gccggggtgg
    26581 aggtcgtggg tcaagttacg cagcggcgga tggcccctga tgccgccact tacattggca
    26641 gcggaaagct cgaagacctg aagaagctag tccaatcgca gaaagcggac gttgtcattt
    26701 tcgacaatga tttgtctccc gctcaaggcc gtaacatcga aaaggtggta cgggctcgcg
    26761 tgatcgatcg gacccaatta attctcgata ttttcgcccg acgtgctcaa acgtacgagg
    26821 cccgattggc cgttgagctt gcccaattgg aatacactct gcctcgactc aagcgaatgt
    26881 ggacacacct tgaacgccag acaaaaggtg gcgtgggcct gcgcggtcct ggtgaaaaac
    26941 agattgaaat ggaccgccgc ctcatttctc atcgcatcca tcagttgcgg tccgagctgg
    27001 aggttgttca tcagcggcgt cggcgagaag tcatcgcgcg aaaggagttt cggactgttt
    27061 cccttgttgg atacacaaac gccggcaaaa gcagtttgat gaatgccttg accgacgcgg
    27121 gcgtgtatgt tgaggatgcc ttgttcgcca cgttagatac ccgcacgcgc cgctgggaaa
    27181 tccccggttg gggccccgta cttttgagcg ataccgttgg atttattcgc gatctacccc
    27241 atcacctcat cgctagcttc aaagccacac tggaagaagc acgtcaggct gatctgctta
    27301 tccacgtggc cgatggcagt aacccggccg tattcgagca gattagctcc gcttacaagg
    27361 tccttgccga gatcggaatc gaatcgaagg acactattct cgtcattaac aagatcgacc
    27421 ggattgatga tccgtcacgt ctccactgca tcctagatcg ctaccccaat gccgtgctgg
    27481 tgagcgcgaa aaccggcgag ggtttgcaac gcctgacgct gatggtgtcc gagttgttgg
    27541 cgcgacagtt cgtcgaagtc gatatcgaaa cgaacgtcgg caacggaaaa ctcattgcct
    27601 ttttgatgac gcacggcgaa ctcatccacg agcgctatca tgatggggat cgtgtgacgt
    27661 tccgctgccg tttgccgggc cagtacttgc cgattgtgga aaaactgatg gagtatcgcc
    27721 ctgacgggaa cgaccaatcg cagtcgaacc gggaaatggc ggcgtatcaa attcactcgg
    27781 ccggcatggt cagcgaatga ctcaagcttg agcctaccac cccctccgaa cgttaaggtt
    27841 tccagattcg tggcactaca aacatgtccc accgaacatt aaacggaatt cgtggcgttg
    27901 ttaccggggc gtccagcgga attggtcagg cctttgtcaa ggcccttctt ccctttcgtc
    27961 cccgcttgct cgttgtggca cgacgcgaag agcgactcca gcagctttct caattaggag
    28021 cacagtacgg ggccgtggtg atccccttcg cttgcgacgt gagccagccg gaggccgcca
    28081 aacgaatctg cgaagtggct caccaaaact ttgagggaat tgatcttctc gttaataacg
    28141 cgggaattgg tgcccttggt cgctttgagg attcgcctcc agaagagctt cgccaggtga
    28201 taaatgtgaa cctcttcagc ctggtcgaga ccacctatta cttgctgcca ttgcttaaga
    28261 aaggacgacg tccgatcatt gtcaatgtga gttccattct aggacatcgc ggtatcccgt
    28321 ggcggagcta ttattgcgcc agcaagttcg cagttcgggg atttagcgaa gccattcgag
    28381 cggagttggc ccggcatggg atcgacgtcc tcgtcgtcag tccagggagg actgccactg
    28441 aactttttga gggacccctg gagaaaggca agacccccgc gtggcccgac ccgaagccag
    28501 tcagcgccga atacgtggcc cagcgaatgc tccgggcaat tcagcgtggg cggcacgaga
    28561 taatccccca tccgtggggg agactgatgg tcctagtcag ccgcttggcc ccgcgnnnnn
    28621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagga ttccgcaggt aaaaaggtgc
    28741 gcaccgagct ggtacggcga ttgctagcaa agctcgattc tctggacgac attagcgcag
    28801 aggataagaa gaagttggaa aggattctgc ttgaagccga tgcagagctt gcccaagctg
    28861 ctgacaggcc gcgagcaacg ggccgtggct tccgctcggg cgatcgcccc actaaggggc
    28921 aacctcccca ggttggccaa gaaccctcaa gggaacaagg agcagtaaca gcccgtggtg
    28981 cgcagacacc gtccacacag gcatccgagg gtcaggggca gcctgaaact cccaccgagg
    29041 ccgagacccc tatggttcgg gcacttctca agcttctcga cgaagtcatt gcagataacc
    29101 tggcggataa ctctgatgat tcccagtcgg cggacgcgct acgcgaaagc ctcctccggt
    29161 ccgatcaagt gttgcgccaa tatctaggcc atgccgcgcg cgccagaatt gaggcggcca
    29221 accgtaagcc gcccgtgcca caggtgtcga atccccggaa aaaaccatcg tctgatgtag
    29281 agcaaaaatg acatcgggcg ggttgcgtat ggaaatccct cgttttgatt gaacttatcc
    29341 aagacgatca ttcgatacca tcaatacgac cggtacattc tcggcgacct tgcccggcgt
    29401 ctttagctgt cttcgataac gcgctaccgg ccaggtagta ctttgtggtg cgatttacgg
    29461 ggcctctgtg tgcggaactt ctatcaacgc cctaccgttt catgaggaac ctcggtgtga
    29521 ctcgccaaac tcggggatat ctttctattc tgatcctggt ctgcttcgtt gtgggatgtc
    29581 gttcgtaccc ctggagtcct gagtcggtgg ctcaaggccc tctagaaggc caatcaagat
    29641 caactcttgc ttacgacgac cagtcgaaaa cggcacaagc ggcccctaaa aaggggtcga
    29701 aagcgcaagc ggaagaagaa gccgagtatg cccgcaaatt ggcgcagggg cgttctttgg
    29761 aaaggctagg caagctggat gaagcacgca agacctatga aggattaatc gcccgcttcc
    29821 cgacaagata cgaggcctac caccggctag cggttgtgag cgatcgccaa atgcgctata
    29881 cggaggccga ggcactttac gcccaagcca tccgtctcaa tccgaccaat ccagacctat
    29941 tcaacgacct tggctattgt tacttgctcc agggaaaacc tgagaaggcg gagagggccc
    30001 tcttgaaagc cgtcggaatg gctcctgcct cggaacggta ccgtaataac ctgggattgg
    30061 cgtatggact ccagggccgc tacgaagagg ccatggagca atttcgccgt gccaatggag
    30121 aggcggacgc ctggtacaac ctggcgtacg ttcaatggat gcgtaacgac atccatctat
    30181 ccaagcaatc cttggagaat gcgctggctt acaatccgtc acacaccgcg gccaaagaaa
    30241 gtctcgccca acttgtgtct ggccaatcct ttttcgcaga aagcaccggg gagcgtcgca
    30301 gtggctatcg gctggcccca gatacggagt ctgagctaat cgcggccaat ggtgcgccgt
    30361 cctcttctga tcagtccgtg acacagacaa cgtttgaaat ggtcccgggc acgctccgcc
    30421 tcggtctcaa ggaccgtccc agcacccaaa cgcaacttga tgtggcgcga tcctcttggg
    30481 ccaaacggct tcaacaagaa acagcccctc ttgagttggc cgagtgaatg ttgaggccgc
    30541 actgttaggg tgcgaaaaac ttttccaaga ttctgcccaa aatcgcctca cccctggcgt
    30601 ttgcgtggac aatgtcgcgc ttgaaccaat gaatatcctc cccgcaggta cgaatgtact
    30661 ggccccaggg tccagtcatg tccaagaacg ccgtgttttc ctcctcggca agtcttttta
    30721 gtcgagaccg aaagtcgttg ccatccttgg gtacctcgaa tgtccactct ggatccatcc
    30781 gcggatcctt gtggccgaaa catggggtca tgagaagaat ctcggggtca ggttccaagt
    30841 tcgcccggac accgcgaatc acttcccgaa cagactcgat atcgtcgcgg tgactaatac
    30901 ctccgatcat gagcaggtcg ggcttatact tgagcacgta ctcttcgagc tggttagggt
    30961 ccttgtaata ccaacaccct gtgctgccac gtactgacgt gattttctcg atgtgggccc
    31021 caggataaag gcgtgccacg agcttttccc atgccgaacg gctcgtatca tttacgatac
    31081 tatcgccaag catgaccacg cgaagggtgc caccgtgttt tagcgtcgca atcgaacgtt
    31141 ccaagttacg ccaccggtct gaaggaggcg tccacgacag gggaaccatc tcgctataaa
    31201 tgaaatcaat tcgctctagc acgggatcgt tctttccgag cgtgcttgga ccattttcgg
    31261 cggcactaac cttttccgtc acaaacgcca ccaatattac gagtaccaat tcagcaaaga
    31321 cgttactcat tgtggctttc atcatatcgg acacctcgca accacctttc tcaccaggaa
    31381 aaaaaagact accacttttc ctctgcaaca cgccaggaca cccaaacacg cgcgacattc
    31441 tgagcacatg cgtctccagt gcaagtccgg ttgcacaagc ccacatctct gccgccagtc
    31501 ggattacctg tgtactatgg gttcttgaaa ttcctatttg aaacacctat gaatctatcc
    31561 caaaaagccc cctctccctt cgggagaggg ttggggtgag ggcaagcgat cgtcggagtc
    31621 catcgagaaa accggcagcg attcgagtca ccctcacccc cggcccctct cccgccgaac
    31681 gggcgagggg agattatggg ataggctcgt actccaatga agcccgaacg atataggttt
    31741 gtgccttggc cgagaacgtg cgggcaccat cctctgtgac cccaacctct acaggcacgc
    31801 cacgctcgtc aaaaatcgtg acttttgtaa acttctctgg aacacggagt cgtatcactt
    31861 ggggaacctc aggattgagc tgagcttcta gagattgttt gtcccaccgg atagagacgc
    31921 ctacctttcc ccgagcacgc agtccggcga tactgccagt ttcccaggct gccggcaaag
    31981 cgggcaagag attaatctct ccgccgtgac tttgcaaaag catttccgca atgcctgccg
    32041 tgccaccaaa gtttccgtca atttggaagg gaggacaatt gtcaagcata ttcggcaagg
    32101 tactttgcga aatcaactcg ctcaacagtt tgtaagcccg gtcgccatcg gccaatcgtg
    32161 cccacaggct gattttccac gccttgctcc aacctgtgcc gccgtcccct cgaaattcga
    32221 gtgatcgtcg cgctgcctgg cacaattcgg gcgagccacg cagcgtgatt tcttcaccag
    32281 ggaaaagacc ccagagatgc gataaatgcc gatgcttgtt gtttgggtca tctttgtctt
    32341 ccagccattc ttggagttgg ccaaattcgc cgatctggtt gggggcaatg cgcgcatgca
    32401 tctccataag ccgtttacgg aagtcagcgt ccacgccgag gatttcggcc gccttggccg
    32461 tcgtggcaaa aagatagcgg atgatctgat ggtccatggt ggggcccatg acgagtccgc
    32521 cctgttcggg cgagttggac ggtccagaga tgagccaccg ttgcggtgaa cgagggtcct
    32581 caaccaaata gtgcatgaaa aattgacacg ctcctttcat gaggggatat ccacgcttgg
    32641 ccagaaagtc acggtcttgg gtgtactcgt agtgccacca aatatgttgg cataaccagg
    32701 cacctccggt cggccaaatg ccgtgattcg ccgcattgat gggtgccgtt ccccgccaga
    32761 gatcaaaatt gtggtgaaga acccaccccg gggcgtcata gtgcaccttg gcggtgcggg
    32821 caccagagat ggctatttcc tctatcgcat caaaaagcgg ttccgcacat tccgacaggt
    32881 tacagacctc agccggccag taattcattt cgacattgat gtttgccgta tatttcgatt
    32941 cccacggagg tgacaggctt tcgttccaaa tcccttgcaa gttcgccggt tgggtgccgg
    33001 gacggctgct cgagatcagc aaataccgtc caaattgaaa atagagaacg caaagttctg
    33061 ggtcgtcttg tttgtcgaaa ttgcggatcc gtaggtttgt gggttgttgc gctgcctccg
    33121 tgcttggcaa ggcaaactgc acacgatcga agagacttcg gtagtcagcc atatgtcgag
    33181 ccagcagctc ttcgtacgac cttgttctgg ccttctcgag gatttcggcg caacgcgcag
    33241 agggatcagc actaacgtcc tcaaaatccc ggtaattcgt ggctgccgtc agcaggatcg
    33301 tcaccgcgtg ggccccggag acttgaaggg cttcatcatg cgtgacaaga gaacctcctt
    33361 ccggttgcac aagggcgcgc acctcaaagc ggatggcact tggcattgtt tcctttgtcc
    33421 gcttatcgat gtagtctgac acctttccgc ggagaatgat cgcatttgag gccgtttgga
    33481 tcgcgtgctc cacatggggt gtcttgagag aggccttgag gccgacggaa ccgggcttat
    33541 cggccgtgac acgcacaacg atgacttggt cgggatagct ggcaaagacc gtctgcttgt
    33601 acgtaacccc gtcgacttgg taactggtaa tggctgttgc cgacgagagg tcgagttcgc
    33661 gccgatatga cgagaccttt gcgttttcac cgaaatcgag gtacaggtcg caacaaggtt
    33721 gatacggcat ttgtcgcaaa ggaacgctca tgaagtgttc catagccagg gcggttgcct
    33781 cttcttgttt cccttcgaac aacaggcgtc gaatctccgg taggtatttg gcagctccgg
    33841 gatgagaata gtcatgtggc gaccccgccc aaaccgtatc ctcgttgatc tgaatccgtt
    33901 cacagtcggt cttaccaaag accatcgccc ccaagcggcc attaccaatc ggcacggcct
    33961 ccgtccactt ctccgccggc gagttatacc acaagacgag cgggttcgag gcaaacgccg
    34021 tcccgctccc tgcatcccgc gcaatcagta agccaatcaa cagcaaagtt tttcccaaca
    34081 gaaatgtcat cgctttaaca ttcatcccgg caaaccttcg gctatcagag aattcgccca
    34141 cgcacaacca cacaatgtcc cctagaggcg catgcccgtg tgaccttgcg ggaaacctgg
    34201 tcgctacccc cttgtgggaa gaccattgta tcgtggacgt cttaccgtgc ggtgtcaata
    34261 gactgaagga aagcttttgg tgtcgccgta agacactttt caccaaggga gaaaaaccaa
    34321 ccgcgttctt ttgcagcccg gaggtttgtc cacgagttaa agggggaaaa ccctgcggaa
    34381 cctgtctttc cgcttggagt tactgcgaag tgccaacgga aacgttccaa aaaagatggt
    34441 ccagaaaaaa ccggaccaca agagagagaa accaatcttg cgcggttttc taaagtgcgc
    34501 aagctactgg tcaaaaagcc cttcgaatgg aatgctgact aagcaccacg aaccggccag
    34561 agaagacacc cagcccgaag aagcgaagta ccggaggtgg gagttgaacc cacacggccc
    34621 gaaggccact ggattttgag tccagcgcgt ctgccattcc gccactccgg cagtccttaa
    34681 cgcatctcgg atactgaata atatcgtcac taagttggcg accgacaagc cccaccccaa
    34741 cgtgccccct cgttctccat cgagtcaact cgcctatctt gcctgaggcg caaatgttga
    34801 tcacaagtaa cggctagatc aatacttgcg atcaactaat gcgcttaccc cgcgattgtc
    34861 ggaagggagg aaatctgata tgctaactgt ttacggccat gatggcccgt aaggggctta
    34921 ttacagccgc aaatgggtca aaccgcgaag aaaaccttct tgggcacggt gatatcttat
    34981 gtcagataca gagtttgaag aaaacgacaa tagcacgggc acaccaggtg ggcaatctcc
    35041 ccagggggaa acccgcggcc gacttattcc gctggctatt gaagacgagc tgaagtccag
    35101 ctacctcacg tacgcgatga gcgtaatcgt cagtcgggca ttacccgacg ttcgggatgg
    35161 actcaagccc tctcagcggc ggattcttgt tgcgatgaat gacctcaacc taggtcctgg
    35221 agcagcccgc gtgaagtgcg caaagatatg cggcgacacg agcgggaact accatcccca
    35281 cggggaagga gttgtctatc cgacgctcgt ccgtatggcc caggaatgga acatgcggta
    35341 tgtccttatc gacaagcaag ggaatttcgg gtccatcgcg gggttgcctc cagcggcgat
    35401 gcgatatacg gaagcccggc tttcccccta cgcggcaatg atgctggagg accttaacct
    35461 cgacaccgtg gattttgttc caacttatga cgagcggcat ttggaaccga cagtactgcc
    35521 ctcaaagttt ccgaatctgc tcgtcaatgg ggccaccggc atcgcggtgg gaatgtccac
    35581 cgaaattcct ccgcacaatc tctcggagat ttgtgatgca ttggtccgtt tgattgatga
    35641 tccggatgtt acaattgacg agcttttgga gattgtcccc ggtcccgact tccccacggg
    35701 tggcattatt tgtgggcgtc gcggtattcg acagggttat ttgaccgggc acggaacgat
    35761 tgtcattcgg gcacgggctg acatcgagga ataccgcgat aatcggtatc gaattgtcat
    35821 tcgggaaatc ccattccaac aacagcgaga caagattgaa gaaaaaattg ccgaactggt
    35881 caacgagggg cgcatctcgg gcattgcgac catccgtaac gaaagcgatc ttaaggagcc
    35941 cgtccggatt gtccttgagc tgaagcgtga cgctgatccg gagatcgtcc ttaatcaact
    36001 gtacgagtat tcccccctcc agcagaccta ctccatcaat ctcctagcgc tcgtagatgg
    36061 gaaaccacgg ttgctcaatt tcaaggaact ccttcaggaa tttctgcgcc atcgtgtaac
    36121 cgtcattcga cgacggacac agtatctgtt gggcaaagct cggcagcgca aacacaccgt
    36181 ggaggggctt ctgcttgcct tggcaaatat cgacgaagtc attcaagtca ttcggacatc
    36241 agaaacacag gcggaagcca aagaacggct catgaaaatc gcttgtccgg cggccatgtt
    36301 agctcgggcc ttgggagagg cgggatttga acaattccgg tccgaacgcg gggtgtccga
    36361 ggagtacttc ctaaccgcgg tacaggccga cgccatctta cgcatgacac tagggcagtt
    36421 agtcaaccta gaacaagaaa agctggctga ggagcatcgc cgtctgctgg cggacattgc
    36481 cgagtataac cgcattctgt ccgatgaacg caacattttg gcgatcattc gagaggagca
    36541 actcgccatc aagcaaaaat atggtgacgc ccgccgcaca gagatcatcg accaggaggt
    36601 ggggtccttc gacatggagg aactgatccc cgatgaaatg atggtcgtgt cgatcagttc
    36661 ggcgggctac atcaaaagga cgcccgtctc ggcatatcgg gcgcaaaggc gaggcggcaa
    36721 gggactcaag ggtgcccgtg ccgatgatga agatcccatc gaacacgtgt tcctggccag
    36781 cacacacgat tatcttttgt tctttaccaa tctcggtcgc gtacattggc gaaaagtgta
    36841 tgaactgcca gagttgcctc gggatgcgaa agggcgggcg attgtcaatt tgctcaattt
    36901 gcaggacaac gaacgggtcg caagctgccg tccggtccga gacttttcgc tgcccaatca
    36961 ttaccttctc tttgtgacca tgaaggggct ggtcaaaaag acggaactgt ctgcctatgg
    37021 ccgtcccgtc cgtggcggta tcatcgccat taaactccgc gaagacgatg aactggttgg
    37081 cgttgtcatc accaaaccgg gggatgagat cgttctagcc acggccaacg gaatggcgat
    37141 ccggtttcgc gaatccgatg ttcgcccaat gggacgggct gcaaccggcg tcaaaggtat
    37201 cacgctggct ccgaacgacc gggtcgtggg ggtggtcgtg gccgaaccag acgcatgctt
    37261 gctaaccgtt tgtgcaaatg gctatgggaa gcgaacgcca tttgggccta actcagagga
    37321 cgaaggtgag gaacttcatg aagatttgga cgaagtgaca gaaggcacgg acgaactggc
    37381 ggcaaaaagc gaggaacttt ctgaggaaga cgaaaccgat gatgaccgcg acgaaccagg
    37441 ttcccaatac tgctatcgga cgcaacgacg tggaggcaag ggcatccgcg acatcaagac
    37501 aggcggtagg aatggccccg tagtcggtat cgtggcagtc cacgacgacg acgaagtctt
    37561 gatgatgaca gcaaaaggga agattcagag gattcgggcc aatgagattc ggaaaaccgg
    37621 ccgcaatgct aagggtgtcc gactgatgac actagatgag ggagaccaaa tcgtggccgt
    37681 caagaggatt ccagccgagc cgaccaacgg ttcagatgcc gaaggcaaca cgccaaccac
    37741 ctcctgattg agccagattt gacacgaaga aaaacagccc gtaggaccca ttgtgacgtc
    37801 agccgcgaca ttgtggaccg gtaatccagt taaatgcgca tgactcaccg tgctctgatt
    37861 acaggtatta ccggttttgt aggccgtcac ctgtatgcgc atcttgtaga gtgtggtgat
    37921 caggttctgg gacttgcacc atccccggtt cccaaagtgc cttcttgggg tcgccttcct
    37981 gacgatggcg agattcttat ctgggacttc gcggaatcaa aagaatgccc ggacgacatc
    38041 aaggcccgca ttcgccagtt tgaccccacg gttgtctatc acttggcagc tatatctgtg
    38101 cctgaggact gcggtcacac ggctccgaat gaaagggcgt gggccatcaa cgtggaagga
    38161 acacgccgca tcatcgaact gttgaatgag accagtagtg gcccgcgatt catctttgtg
    38221 agcacaagcc atctttatcg cgtcccgcag gacaaggtga catttgttga tgaagagtac
    38281 cccgctaatc cccgcaatgc ctacgcccag accaaatggg cagcggaaca aattgtacaa
    38341 gctgccgtca aggaggatag gcttaatgcg atcatcgttc gcgcatttcc ccattcaggt
    38401 ccaggacaat ccgagcgaat gatgcttccc tcatgggctg cccaatttgc gcggggcatg
    38461 cagacaatcc aggttcacac gttgcaggcg acgattgatc tgtgcgacgt tcgggacgta
    38521 gtgcgggcat accgccttct ggcagactct gcggtgcctg gtgaggtcta taacatcggt
    38581 tctggtgtgc cgcgcacaag tgcccaggtt tttgagctat tgcgactcct cgccaatccc
    38641 cattgtgagg ttgttgaaac tcatcctgga atcaagtatg atccaatcgc cgatttgcgg
    38701 aaggttcaaa gtcacaccca ttggaagcca gaaattcccc ccgagcagac cgtggccgat
    38761 acctaccgct ggtggttagc aacagtaggg cgcaagtagc ggtaccgttg ccctcttgct
    38821 ttaggactcg gctgtcggaa acgtaaacgt ttttgttctc cgcggggatg gtgtagcgtc
    38881 cagttcggaa agacagcgtg aagggagaga cgccatgatg aaatcgaaca tcgtttgttt
    38941 cctacctggg gcgatgctag ccatttcgct ggcggcatca accgtagccg cggaaaccac
    39001 gatttacctc tcgccgcaag gaaaagatac taaccgggga acggcagatc agcctgttgc
    39061 cacgctcgcc cgcgccatgg agctcgtcaa ggccgtccga cagcgtgacg gggctgcagc
    39121 cgacgtccgt gtccttctag cggaagggat ttatcgaatt gccgaacctg tcacgatctc
    39181 gccggaacac tcacctggcc ccggcaaact ggtaatttgt ggtgaagccg gcaaaaaggt
    39241 tgttattagt ggcggccgcg ttatttcggg ttggcaaaag gtcgggaatc gttttgaggt
    39301 tgtcattcct gaggttgctc aggggaaatg gatttttcgc gagcttttcg tgaatggtcg
    39361 ccgggcgacg cgagctcgat cgccgaatga gggatttgtc cgtatcgaca aagcgggccc
    39421 agaccgccgc accagcctgt ttatttatcc gcaagatgca tggttgttca aaggcaaaat
    39481 ttccggggcc gaatttgttt atttacacga ttggtccaca tcgcgggtga agattgcggg
    39541 ttttcacccc gatacccttg aggttacttt ttcgcaaagg gtggggtgct ccgctcctca
    39601 ttacgccatc ggaaactggc cccatgcccg ttattttgtc gagaacagtg aggatttcct
    39661 ggacgccccg ggtgaatggt tcctggacga tggcacaggc aaactgatct atttgccccg
    39721 ggaaggagaa tccttggaaa cgctggaagc cattgcgccg gttgctcctg cccttttcag
    39781 catcgtcggg gatttagacc acggaaaacc tgtcaccaac ctcaccattg aaggaatcac
    39841 ctttgagcat tgccggttcg atctaccggc tgccggttat gccgccggtc aggcgaccgt
    39901 ttacgaaccg cgggtcaacg gaggagctgg ccgagagatc atgcccgcgg ccgtgacatt
    39961 tgattttgcc aagaactgcc aactgactcg ctgtcacttc caacacctgg gctcatcagg
    40021 cgtatggttt cgccggcaat gccgtcagtg taccgtcgag aggtgcgttt tcgacgatat
    40081 ttcggggaat ggaattaata tcggcgagac gatgacacgt cccaagtctg aggctgcggg
    40141 ggattgggaa tcaccttacg ataacggctg cacgtgggga atcatggtcc gggataatcg
    40201 gatcacccat tgcggccgcc agttttttga agctgtcggc gtgtgggtcg ggatcgctgc
    40261 tggcaacgtc atcgagcaca atgaaattgg gtacctaccc tacacgggcg tatccgtggg
    40321 atggagttgg aatgactcgc ccaccggatg tcgagataac attgttgcac gaaaccacat
    40381 ccatcattgc atgctcatcc tgcatgatgg cggcggtatc tacacactag gacgtcaagc
    40441 gggtacgaaa ctcttgggca acgtcattca tgacattcct cccaacgaag atggagcgga
    40501 aagcaacggc atctttatgg acgaaggcag cagtgacatc gttgtggaag gtcaaacaat
    40561 tttcaatatt gggcgaagtc ctttacgctt ccatcgcgca ctggaaatca cgttgagcca
    40621 caataccctt gtgtgtttgc caggagtgcc gccttatcgc tataatcgca ctgatcccaa
    40681 aacgctgcgt tttgagaatg accacatcgt cgaggccgaa cactggactc ccccgagtca
    40741 aattcttggg gaagtcggcg tgcgcgaagg tcgatgaatc tagggtttgg ttttttgggg
    40801 aggtgcttta tgtttcgaag tcgtgtggtt ccgctcgcgc tcgtggcagc ctcttgtgtt
    40861 tgccacatcg gccaagtcgt gcacggagaa gaaagctcac gtggtcgcta caatgtgctt
    40921 ttcgtgattg ccgacgattt gaacacagcg ctatcgacct atgggcatcc gatggtgaaa
    40981 tcccccaacg tggatcgtgt gcaacgttgg ggcatgcggt tcgaccgagc ttattgtcag
    41041 tttcctcttt gcaacccaag ccgagcgtct tttctcacgg gacttcgccc ggatcatacg
    41101 cgtgtgtatg acaatgccgt tcattttcgg aagaacgttc ccgactgcgt aaccatgccg
    41161 gagtttttcc gaaaacaaaa ttatttcgtt gcccgtgtgg gaaagctatt tcactacggg
    41221 gtgcccgggc aaatcggcac aagtggcctt gatgaccctc aatcttggga tttggtcatt
    41281 aatcctcgtg gacgcgacaa ggacgaggaa gataagattt tcagcctgga accgggcaag
    41341 tttgggggta cactcagttg gttggccgca gacgggaccg acgaagagca aaccgacggc
    41401 attggagcca cggaggccat tcgtctgctc gaagagaaca aggacaagcg atttttcctc
    41461 gcggtcgggt tttaccgtcc gcacacaccc tatgtcgccc caaagaagta cttcagcctt
    41521 tatcctcccg aattggtccc cttgcctacg atcccggaga actatttcag tacgatccct
    41581 gccctcgcca tcacgctgaa acctgaagaa aaggcgatgg acgaaaagac aaagaggcaa
    41641 gccattcaag cctactacgc cgcgatcacg ttcatggacg cacaattggg ccgcgtgctc
    41701 gacgccttag aacgacttca tctgcgagag cgtacgatca tcgtcatgac gagcgatcac
    41761 ggctatcaca tgcatgagca cggcttgtgg cagaaacgga cccttttcga agagtcggct
    41821 cgcgtgccgc tgcttattgc cgttccagga atgaagacgg ctggccaatc gactcaggcg
    41881 attgtcgaaa tgatcgacat ctatccgacc ttggtcgatc tttgcggatt tgaagttccc
    41941 aagaacctag acgggatgag tttggtaccg attctcaata atcctgccca tcctggcaag
    42001 tcacatgccc tcacccaggt atcgcgcggt ggcggaaaga ctaggcttgt catggggtat
    42061 tcggttcgaa cagatcgctg gcgctataca gagtgggatg gtggcaaaca ggggcgagag
    42121 ttatacgacc atcaagatga tcctcaagag atgaaaaatc tggccggtga tgaacgatac
    42181 gaaaacgtca tgaaggaatt aagtgccctc atgccaaaac cggcggaata atagcgggat
    42241 gactgttggg ttgtttcggt actttcaatt ggagaggagg acaacatgaa gaagaaagtg
    42301 accttgattg cggctctcct acttgcgctc gttggtggtc ttgtcgtaca actgtacgca
    42361 gaatctcaag cgaaattcag ccacgccggg caagttcgtc acgtcgtcct ttttaaattc
    42421 aaggatggca cctctcctga agacattgcc aagatcgaaa aggccttcgc agccttgcca
    42481 agcaagattc ctcagatcgc cggctttgaa tggggaaccg attgcagcgt ggagaacctt
    42541 tctcaaggtt ttacgcattg ctttatcttg acgttcaaga atgtccaaga ccgggatgcc
    42601 tatctacccc accctgagca taaagcgttt gggcagttgc tccgtcctca cttggataaa
    42661 gtcctcgtga tcgactacgt cgtacgagag tagttcaact cgatttgatt tcgcgacatc
    42721 ggcttcggac aggtggacca tgaggtaccc tcccctttgg agggttttta tttgcgcgaa
    42781 gcggtgtcgc gtcgaaccac ccctggttcc acatcgaggt caagcggcgc aaagagcttg
    42841 gcacgccagc gttctacggc gatcgcgccc atgacagcat tgtccgtaca cagtcgcagc
    42901 ggagcgatga tcagccgaat attccgctcc cgtgcttcct cttcgagtcg ctcccttaag
    42961 cggcgattgg cggcgacccc accgccgacg cagagcgttg tcaaacccgt tgcctgcaag
    43021 gcctgcaagc tcttgcctac caagcaatcg accacggcct cttgaaatga tgctgccaga
    43081 tcggcgatcg tttgctgtgg cagatcaacc tgagaacaat cgtgtctccc cggcccggta
    43141 atggcatagc gaacggcggt tttcagtccg ctgaaactaa aatctaagca atcgggatta
    43201 tcaagaaagg cccgcggaaa tttgtatttc ttgggatcac cgccactcgc cgccctatcg
    43261 atggaaggac cacctggata aggcagcttc agcatggcgg cgactttgtc aaacgcttct
    43321 cccgccgcat catccaaggt gcagccgagc aattcaaaat ccagtgcatt cagacagcga
    43381 taaaggcacg tatgcccacc actgatgacc agccctacac aagggaagac atcctcgcca
    43441 gcagccatcc ggcaggcgta gatatgggcc tgcaagtgat tgatagcaat caacggtcga
    43501 tccagagcga cacacaacgc cttggcggcc gttaggccaa tcaaaagtga acccgccaaa
    43561 cccggcgtgt aggccaccgc caccgcggta atatcatcca gtttgacacc tgcccgcgaa
    43621 atagtctcat gaatgaccgg tagaatacgc tccacgtgag cgcgggacgc caactcagga
    43681 accacacccc cgtaccgttc gtgaagctta tcctgggagg caaccaccga ggagagaacc
    43741 tcaagttgat ccgtcaccag tgcagctgcc gtttcatcgc atgttgtttc aattgtaaga
    43801 aacaatggca ctttgccgct ccttttgggc ttttgaccac actgcgcaag ctttataatg
    43861 attctatatt ctccatccgg gcgttggcag gtgaaggtct ccccgcttgg ttagcaaatg
    43921 gcgaatcgcc tcgtcacact caaagaggaa ggctatgcga gcgtcaattc aggaggtcat
    43981 cttggggcct ttgaatcccc aacagcggga ggccgtccag catggtgagc agcccctttt
    44041 gatcgtcgct ggagcgggta cggggaagac tgccaccatt gtccatcggg tagcctggct
    44101 cgttgcccac ggggtctccc cacagtcgat tatgctcctg acattcacgc gccgcgccgc
    44161 tgaggaaatg atccgacgag ccagttcact cctcagccgt ttgggtcttt cgcgggaatc
    44221 aacccatacg tcccacatct ggggagggac attnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44341 nnnnnnnnnn nnntggacta cgacgatttg ctcatgcaat ggttaaagct attgtcccac
    44401 agccaaatcg cagaacatat ccgcaaacgg atttcgtttg tcctcgtcga tgaataccaa
    44461 gatactaatc caattcaggc ccagattctt tacgccttat ctccttctgg acaaggcgtc
    44521 actgttgttg gcgatgacgc tcagtcgatt tattcttttc gtggtgccac agtcaagaac
    44581 attctcgact ttcccaagca ttatccctca gcgagaattg tcacccttga acagaattac
    44641 cgcagcacga tgcccatcct gagggcggcc aatgccgtca tggcgtcggc gaaggaacgc
    44701 ttcacaaaga acctatggtc agaacagcgg gcgggttgtt taccgcgcct ggttacctgt
    44761 gatgacgaat atgagcaagc cgatttgatc atccgcgaag tcctcgctca tcatgagctg
    44821 ggcactgatt tgcgacgcca ggccgtgctc tttcgggcct cgcaccacag cctcccccta
    44881 gaagccgagt taacacgccg ggagattccc tatcgcaaat atgggggact taagttcctg
    44941 gaggctgccc acatcaagga ccttctggca tttctccggc ttgccgaaaa cccccgcgat
    45001 gtggtcgccg gcgtgagggt cctttgtttg cttcccggca taggcccggc taaggctcag
    45061 aagttgctgg agcaggtggc atcttctccg caaccttcct tcgatccttg gaaagagatc
    45121 aatccatccc gtgattctgc cgatatctgg ccatcgtttg tctccgtcat ggaggacgtt
    45181 gcgagtcgca atctccccgt tgcggcccaa atccatcggg tgcggatctt ttacgagcca
    45241 atgttacccg tgttgtacga caatccgatc cctcgcgcca aggatttgga gcagttggag
    45301 cagatcgcct cccgatataa ggaccgccag acattccttg cccagattgc cattgatccg
    45361 ccgaattcca cgcaagactt cgccggcaag cccgatttga atgacgactt ccttatccta
    45421 agtacgattc attcagccaa aggcctcgaa tgggacgtcg tttacatcat caatgcgaca
    45481 gacggccaca tcccttccga catggctacc ggctcggagg cggaaatcga agaagagcga
    45541 cgcctattct atgttgctat gacgcgagcc agacgcgttc tttacgtcta taccccggtt
    45601 cggtactacg tatccccatc ttttgccgac agccgctatg gcatgggcct tccaagccga
    45661 ttcctgacac ctgaggtcgt cgagtgtttc gagcgatacc acggcgatgt gcttgacggc
    45721 gagatgcaag caaattacca acagtatccg aatgatctgc catcgggcat tggcaaaccc
    45781 atttttgctc gcagatcgca gaaggccgga ggcgacatct cgatggaatc gggctgagat
    45841 ggtcagggca agagtgcctt ccatcagcca actcgccggg tgactacgtg ggatagacgg
    45901 gttgcggcaa gttccaaatc cacccaaagc ctgccgccca acgtagaacg agtgcgatca
    45961 gcgagaaaaa cgtaaaatgc aaaatcccgg cggaatagtc cagttccatg gccgcgtaag
    46021 caacgaatcc tccagcagca aaggcgggcg gtaagacaat cgcccacgtc cacagctctt
    46081 cggtcacaaa attggcaaca agccagttaa acaatcccca aatgatgaga taacccaccg
    46141 cacacgccag agaacggatg atcaagtttc tcccccgaag cggctttaga tcatcttgat
    46201 gacggaggaa ctcgtaactc atcgccgccg caggaactcc cacgaggact agtccgatag
    46261 catgaagcca cacttgctga tggaagatat ccaactggcc caagacaagc gtaacgacca
    46321 agacaatcac aaacgccccg acggcaatca agatgtgaac gggtttaatc gccgtctcaa
    46381 ctcgggcgag cggctttgtt gttagttgcc cagagatacc ccggcctcca tcggcaaact
    46441 gctccggttc ctcaatgacg acctgctcgt tcagtttcgg gatttggata atagtcttac
    46501 atttgggaca agggccgctg cgcccagcaa attgatcact gacagtgaaa cgagtgcgac
    46561 atcccggaca gacaacgaaa atagccataa gaaccctcat gtcctcaaga aatgaccatc
    46621 ctgctgtctc ttttagtttg gcagaggtgt ttcccttgtc aaccgccggc taggcgcgct
    46681 tgagcgtcga cgagcccgaa caaaatcaag atcactttct tcgtggtggc ccagcacaac
    46741 cggcccactt cgttgtaacc atctgatgtg cttacctttg aaactcacgc gcgctcgtgg
    46801 tgggcgaaca tgatggatca ccaataggct ccacacgcga agtaacggca cacctccctc
    46861 caaaattggt acaaagtgcg tcgtatttct tcaaatcgga tggtaatccg cgagcaaaac
    46921 tgaatggcca aatcgtttgg cagctatgga atttgtccaa atcggatgcg aatccgggcg
    46981 taaaactgct tcccatgcgt gagggttaca tcacaaacgc ctcgctacca agctagaacg
    47041 cttgcatagg ctgtgctcga atcgtacgat aggcacagtt tgatgagcac tccaaacctg
    47101 cgagggtgtt tggcgaatac ggagatcaga tatttcacgt caacaataac aatccgcatg
    47161 aggtaaaaaa caatgaaggt aactcgctcg gcgctctcgg ttggacgcat gcaacatttc
    47221 tcccaactgg ccatcggtct gttggtcatc atatggtttg ctgctccctc ggaggcccag
    47281 aaccgagggc tttggacgcc cgaccaagaa accgggatca ccgccgatag ggccacaatt
    47341 atctacgaaa aattaccaga tggtagaaag gttgctcatg tccaggccaa gggcgtggcg
    47401 gaaaatgtgg ccattcgctt tcgctctcaa gatggttctt gggatctgag tgacttttgt
    47461 tacgtcggca ttcaggtgca aaaccggctc gaaaaagaga ttcgtgttca gtggcgggtt
    47521 cgagccaaag ataagtcact gtctcgcgat tatgtcatga aaccgggcga gtcccacttt
    47581 ctacggttaa ctctccggag ggccgtacca acacagttga aagacgtctt ttttggaatg
    47641 cggggctacc cagcccttat ggatccggag cgaggtatcg ccgtcaacgc ggtggaggaa
    47701 ctggccctca atatccgtgg tcctgttgag ggcacttttt tgacaatcga aaatgtggct
    47761 gcctctggga aattccaaaa gccccagtgg tggaacggga taacgagccg gttgtttccc
    47821 atgattgaca tgttcgggca gtaccggcac gctgactggc ccggtaagac gcactccatt
    47881 gaagaattgc gagcccgaat tgcagaagaa gatcaggatt tagcgcaaca tcccggtccg
    47941 agcgactgga atcaatttgg cggttggatt ggcgggccaa aactcgaagc aaagggccat
    48001 ttctgggtaa cgaaacacga cggccgatgg tggttggtgg atccggaggg gtgtttgttt
    48061 tggtctcacg ggatcgattg tgtacgtgca acgacggcca cgaccccaat cacggaccgc
    48121 gaacactggt ttgaattgcc tgctcgcgat ggaccattcg gccttttcta cagtcgggcg
    48181 ggatgggcac cgcatgggta ttaccaaaag cgttccccct atgagcaatt ctgcttttcc
    48241 ggtgccaacc tgctacgaaa gtatggagag aattgggaag cggccttcaa cgagcgcgcc
    48301 catcgccggt tgcggagctg gggactgaat actatcggaa actggtccga tccggccatc
    48361 taccgcgaaa aaaagacacc ttatgtggtg accttatcgt ctggaaaacg aaaaatcgaa
    48421 ggatcaaccg gttattgggg tcaattcccc gaccccttcg acccaacttt tgaccaacct
    48481 ttgcgtgagg caatggcgcg agaagcgaac caatcagtga tcgacccctg gtgcttgggt
    48541 tattttgtgg acaacgaact tagctgggga gacgatgtgt cgctcgcctt ggccacggtt
    48601 cgttcgccag cagaacaacc ggccaagaag gcttttatgg agatgttaaa gcagaaatac
    48661 ggtacggtgg agaagttaaa tgaagtatgg ggaaccaagt accaatcttg ggagaatttt
    48721 ctggaagtca ctgatgagcc tgatcgcaag aaggcggaaa gtgatcttgg cgcattctac
    48781 agcgagattg cggagacata tttccggcgc tgccgagagg ccattaaggc cgttggaccg
    48841 aacaagctct atcttggttg ccggtttgcc tgggttaatg agcgggccat tcgcgccgcc
    48901 gccaaatact gcgatattat cagtttcaat cgctacgaag ttagtgttgc gagttttaca
    48961 cttccccaag gggtggataa gccggtgatt atcggcgaat tccacttcgg ggcactcgat
    49021 cgcggcatgt ttcaccccgg gctgcgcgaa acggccgatc aagtcgagcg tgccgaggcc
    49081 tatcgtcgat atgtccagag tgcgttggat cacccccaaa tagttgggac ccattggttc
    49141 caatacatgg accaagccac cacgggccgc ggtgatggcg agaattatca gattggattc
    49201 ctggacgtct gcgatacacc gtatccggag atcattcagg catcccgcga tgtcgccgcc
    49261 gagatgtatc ggcgtcgggc ccaccaccaa taggtgacgc cctctcgaaa gccaattttc
    49321 ggcccgctcg agaaacggcc tactgataac ttctgggccg agcgtgattt actccgtttg
    49381 cattggccct agggtgccaa acgcggatga gctttgcccg ttcgggaagc aagccatggc
    49441 ctttaccggt ccctccaaca ccgttttcct tcccaagggg atcaattctc ggcatcgagg
    49501 atcaggggga catccatttg ccgtccttgc cgaatgatgg tgaaaatcgc cttctcgccc
    49561 ggttgcttac tctccaagac ggtcagaagg tcatccaccg tctttactgg ctttccatca
    49621 accgcaatga ttaaatcggc cgttgagcgg tcaatgacag tggattcaaa gacgagcggc
    49681 ccctgccggc gagtcctttt cgtcagtcgg aatccttgca gccctgcccg ctccgctgcc
    49741 ccgcccggtt tgagggaggc gattaagatg ccctggtccg tttctaacac ttgaagaatt
    49801 ccggcatcag cccggatgac acgtcccgtg cggatcaact ggggaactac cctggcaatc
    49861 gtgttcacag gaatggcaaa tcccacgccg gcgctctcac cggttcgact cgcaatggcc
    49921 gtattcatgc caataaccaa cccgtgactg tccaagaggg gaccaccgga atttcccgga
    49981 ttgattgcgg catcaatctg aatcatttgc ttcagcatgc gttgactacg tggattgggt
    50041 agcgaacgat taaggctgga gatgattccc gtcgttaacg ttcgttccaa tccaaacggg
    50101 ttaccaatgg cgaaaacccg ttgacccact ttcagatttg ttgagtcacc aaaggtcact
    50161 gggaacaact tctcagatgg tgcatcgatt ttcaggacgg caatgtcact cggcggatct
    50221 gcacccacta gctgggcatc ataggccatc ccatcatgaa gcgtcacaga gatagcccgg
    50281 gcattttcca ccacatggta gttcgtgagg atgtgtccct gccgatcgat gacgctacca
    50341 ctcccctccc cttccgtgat gatattcatg aagaacaagg ccccttcctg aatactcttg
    50401 gttcgaatat taacgacgct cttgttgacc ttttcataga cggcaatgtt gacgatctca
    50461 tctggggtgt actgtcccac tggaggcaga aaggcctccc agcctggtgc actttgcgtg
    50521 aagacaaact ggccttgtgc agtgcttacg gtccagggag tcggtgatgc agtccacgtc
    50581 ggtaggcttc cgggaccctc ggacccttgt gggaaccatg gttcggcaaa ggccgcttgg
    50641 cgatcttgag gttgatcgat catgtaccga tcaccaccgg tcaaaccgga cttcacggcg
    50701 gccacagctg ccaacgcgcc gaccgtaacg ctgacaagac aggcaaatat caaacgcctc
    50761 ataacgacgc ccttacgcta gtcccttgat caatcatccc gttgtttacc agagaggtcc
    50821 acacccccat catacgcgaa gatcatgtct tggtgtttgc ctccgcgttc tcctacttta
    50881 ccgcacggcc ttttcatcgc aaaggtgatg tgcgtcaaag ccagaatggc aaattgggct
    50941 cgcctttcac acgaagcgac ccacgactga cgcagatacg tcgttggcgc gaacaataca
    51001 agcaatgccg tcaccacaac tttttgtgcc cgccaacgaa attcgccaaa atcttgaggt
    51061 tgtgttgaca agcgaacata ggtcgctaca ttcttcatga aaacgggctt cgcgagcgtg
    51121 ccaagtcatc cgtatcacgt cagcagtggg atggcacgcg tcacatttgg cctacgcagg
    51181 gatggatgag gcaggacgac aagagcgcgt cacgtcggca aaagcgttga aactctatct
    51241 gctgacatag tctaacaaac gagaggtcgt cgatagccca cacaactcgc atggaggcga
    51301 tgctggtgat cagagttagc aattctttct tgtttcgcct tttccggact cttccgaagc
    51361 tattgttcca ggcccatccc caacgtgcct ctcccccaaa cgaccatctt aaggactgtg
    51421 ctattcagac ttactcttga acgttcttcg tttgacgagt tcaatcggta agccccgttc
    51481 cgtggtcatg tctcgtttct caggcaaagg atcgcttaaa caaggaggta aacaatgcgt
    51541 cgcaagcctc tcattggtct gaacatggat ttcggcaagg tggaatcgaa aggatatgtc
    51601 gcagaacttc cggccggcta ttttgattgc ctgattcggg cacaagccgt cccggttgcg
    51661 atcccaccac ttgtccatcg ccgtgatatt gaacaagcgc tggatgccgt tgatggcttc
    51721 gtgcttatcg gcggcgacga tctggatcct ttacgcgatg gctgttatca ccatcccacc
    51781 acccgggtga tgcacccccg ccgggaagac tttgaccgcc ttctggtcaa gctcatcgcc
    51841 gaacgtcata tgcccgttct gggaatcggt gctggcatgc aactgctcaa cgtgtcacag
    51901 ggtggaacac tgtcattgaa tatccgcgac gacttctcca atgccatgga tcatcgggat
    51961 ccacatgatc cctatcatcg gcacgggctt gttgttagcc ctaagacctt cgcgaagcgt
    52021 gtctacggcg atggtgagat tcgagtcgct agtcggcatc acatggccgt actcgacttg
    52081 gctcccatct tcaaagtgtc ggccgtggca cccgacggga ttatcgaggt cattgaaagc
    52141 caggacccgg attggttcgc cattggcgtt caatatcacc ccgagtgcga ggcggcttcc
    52201 gcactggacc tgcgtgtctt tgaagaattt gtggctgaag tcgtgcgtcg ccagagcgcc
    52261 atgcagcggg cggttgcgta accatctcct cgatgggaag gtggcggaaa aagccacccc
    52321 tggctgctcc ccgtttacac actggctgtc gcaagctctg caaccgactc ccgttgagaa
    52381 gcagccgacg tatctgcaag aaaccgcatc atcaccgacc agcgccctgt ggcgtcaggt
    52441 gtcacccacc agtgcggttg aattgctacc gactggtgga ccagttcaaa accgccttct
    52501 gattgactga ctgacgccac ggggaacgtc catagatttg tcgggcgaga aaaggccagc
    52561 tggagtcgca agcctaacca actatccact aaggccaagc ctggggtttc ctcaaggtcc
    52621 aactgggtcc ccagatgccc gagtctcgct ccctccaggc tttcaaagaa acggtcatcg
    52681 gcattggcag gaagtcccgc cacgttcaac tccacggcaa aaagtagtgg ccgatcggcg
    52741 ggaagatttt ccaggcgata agcgacctca agaatgggac tgccctcact caagaggatc
    52801 gccttagtta cccgcacggt ttgatccaaa accgtggcct cgcggctgag cgttactcgg
    52861 acacgatcag gattggcgtg aatcgttgtt tcatattgga ggttactcaa atcggcccag
    52921 taaacagctt ggcccgacct cacctgttcc agtgtcgtct cgggactgta gaacatgtcg
    52981 atgagtgact tccgcggata gcggtcatac tgaatctgtg actccagacc agcctgtttg
    53041 aagacgacac gatcatggat gctagcacac ccctcgtgat gaccgctcgc acccgcgcgc
    53101 accttatcgt gatagacttc cggacgccgc gtcatcgtgg caagcaggtt tcgcctgatg
    53161 gaccggacat cccattcgta gagttggcca ccacggaagg gcgcaacgta ggctaccatg
    53221 tggtcattgg caaggcgaac ctcaggctgc ccgtcaaaat caaaatcctc ggcccgcaca
    53281 tccacccagg cactttgcga acgaaaatgc tgttcgatga gattgtcagc cgcaattagg
    53341 tgttcgtaca ccgcgtttcg gaggtgaggc aaataaatcc caccgaaggc cccgtgccaa
    53401 taagggcagt tgcattggcc acgatataac tccaattgtg cgtctcgcaa ggtggagtca
    53461 gcgggcgccg agcttatcgc attttggatt cgccgactga cctccatcat gcgtgcatac
    53521 atttcgttcg cttccggata tttgacgcgg aagtttcgcc aatagccacc ccggatgaaa
    53581 gctctcacgc gagaccagcg gggatcaccg ctcatctcat gacgcagcga ctccagttct
    53641 ctttgttgtt cggtgggcaa ggcccattcg gtcatttcgc ggtagctgca atcgggcaga
    53701 tagattttgc ccagaggcga aacggcgtca taggcctctc cgagggtgca cactcgtagc
    53761 caatcctgtt cacggaggag ggcctcgaag aaccgttcga gccagcggtc ttcgtaaaca
    53821 tgtttcttcg tgtccggcca ggtgccgaat ttttccccat catcaccgaa gaccaccacg
    53881 gcgttggggt gcttttcacc aatctcccga aggtattcaa tcgtgacgtg tggttcggca
    53941 aagggaatgg tataacgaag gcgttcactt cccggaaaga ccgccaacag gcgaccctcg
    54001 tcttcggtca aataatagcc gtacagttgc tctttccgaa gcccggctgc cttgaaatgg
    54061 taatcatcca aaacggtata ttcgataccg gcagccacga tgtccttggt gaaggattgc
    54121 tcccaaacac gttccggcac ccacatccca cggaccttag ccccaaggcg gttctcgagc
    54181 caccgggtgt agtggcggat ttgtccgacg cgatcgtggg agggaatcat tgcgagaatc
    54241 ggttcataaa acgggccacc gatgatctca attcgccctt ggttaaccaa gccggcaatt
    54301 tcatccagat agccgggatg aaccgagtcg agccattcga tcaacggacc gctggtgtga
    54361 agagaaaccc taatcccctc ccaacgtttc aggacttcca aaaatgggcg gtaactgtct
    54421 tcgtatgcct gttgaaaaac atgatcaaaa ttgcctacag gttggtgatc atgcaacaaa
    54481 agaacaaggc ggaccctagg agtggaagac gtcataggtt gattcatcgg tgctactcag
    54541 atgttactct agaccctcag cggacagaca gaccaatgga cgacaactct cgagggctat
    54601 gccggaaacg gaggtggggc tgcgggacag ccatcgcggg gatcgagagg ctcggtgaga
    54661 tcctcgcttc tttttatcta gccctcttta gttactgtat cgacaatcac aacgcatata
    54721 ctccagatta cctaggccag atattttgcc agacccgtta actcgcttgc ccttcgcgga
    54781 gggatcttgc agcaaattcg ggaaataccg agttgatatg gacaccactt ccccattcaa
    54841 aaccggcagg tcgcgcggtg ctgggcaaga tttcgatatg ccaatgataa aatggaacgt
    54901 cgtcatgcag gggtgcatca tgaaggatga cattgtagcc taccccaggt atggtctgtt
    54961 cgatacgctg aatgaggtct ttcgtgatct gaatcagttc gccccatagc cctcgctcca
    55021 agtctgaaaa cctcgcattg tgctggcggg gcagaatcca ggtctcgtag ggaaagcggg
    55081 cagcaaaagg acacatcgcg agaaagtgcg gtgtaatttg gacagtacgg acggcttttg
    55141 ctatttcata ctggacgatc tggcaataaa tgcagctttg atggccccta aagaactcgc
    55201 ctgtcccggc caattccgct tcgattgtcg ggggaatcac cggggtcgcg atgatctggc
    55261 tgtgggtatg ctccacagac gcccccgcca agggcccgac gttcttgaag accatgacat
    55321 accgtagatg ctccttggat ttcaatgccg ctaatcgaga cattagtacc tcaacaacgt
    55381 cgagcatctc ttcggcggtc aaatccgaaa taccctgcac gtggcgaggg gtctcgataa
    55441 ggacctcgtg aactccgaga ccggtccttc cccgataaat cccggattca attgcgcaat
    55501 cctcactgtc aactagcgcc ggatatttat tggggacgac acgaactttc caagatggct
    55561 cctgaggaga tgccgaaaca ttgggcttaa tgagggtggc tgggggggtg agctcttcgt
    55621 tcccggggca aaaagggcac gggccatggc gaatccgcct cggccgcgtg tcgaattctt
    55681 ggggacgttc tgctcgctgg ggggatatga tcacccattt cccagtaatc ggatcttgcc
    55741 ttagctcggc gtttcgctca gcttttgatg agaacgaaaa catctttgcg tcatgcaccc
    55801 tgttgctatt cacgagtcaa cggtagcaat catgctcaaa aggatcaatt gagtcgcgag
    55861 aatctttcca ttatcgcggt cctcacgtta acgaaaaggc cggtttcttg cggggcctgc
    55921 ttagagccgg taaactgggc aaacatgttt cttcactacc acccagcgca accctgagac
    55981 tcgccgctga atcggtggtt gaacgaggca gacagaatgg gctcgggtgg aatcggtgcg
    56041 acacctctga cgacgcgtgt tgacccagat accagcagac gcttcgcaat tcaaacaatc
    56101 aggtgcgagg aggtttgaac atgaattctt gggcgccgtt ccctttcgta cttcaaactg
    56161 caattgtcct cgctgccagt atatcgactg gtcatcaact ctttggccaa gaggaaccaa
    56221 tggttccgcc cctccaaatt tatccccagc cgtatagcca ggcggctttt gtttttgttc
    56281 ccatcccgat ggaactcgtg aggtttcatt ttggaggaac acttttccgc ccgttccttt
    56341 atcccattcg aagcccctcc ggccgggaac taacaaggat ggggcatccg catgatccga
    56401 ttagccacag tcaccataat tcggtgtgga tgtcgcatgc tgatgttaat aattctgatt
    56461 tttggtctga tcgaacagag gcccgcatcg tttgccgcaa cgttgttgaa tatggagagc
    56521 aacccgggac tcccggtcag gatggtagtt ttccgacagc gtggcttttg gcggatattg
    56581 aatgggtttc ggaaaagggt acgccgctgc ttaaggaaga gcgacgcatc acattgcaac
    56641 ttttcgaaag tgcggaaaaa tcctggcgaa attcccactc agccctcgcc ggcggatatc
    56701 taatcatcat tgactcgtca ttccgccccg cgacggaaag tcctgtcact tttggacaga
    56761 cgccctttgg cttaatcggt gtccgcatga ccaagaccat tggggttcgc gacggtggtg
    56821 gccgaatact taatagcgag ggcggcgtca atgaaaaagg gaccttccgt ctccccgcac
    56881 gttggatgga ctatagcggg ccggtcacgg tcgatcaaaa ggccggaatc acgcttttcg
    56941 atcaccctaa caacccggga ttcccaaatc cctttcacgt ccgcgatgac ggttggatgg
    57001 gtatttgctt gacgctcaat ggaccgatca cattggaaaa agaatcaatt ctcaaactcc
    57061 gatacggtct ttggtgtcat gatggggttc ccggcaaaga agagattgac aacgtatttg
    57121 gcatatacag ccgattggct ctacccggcc aacagcggca acagtgacac agcgtatctc
    57181 cccggtttgg aacccaaaac ttcgggagtt gtcaacgctc gtggctagtt ctccgattgt
    57241 ccgtcaagcg accccagcag aatagaagct attatccttt ttcgattggg cacggattgt
    57301 ctcctatgga tgttcgcaca ttaccagact ccgaacgttc tccgcacgct gcttccgaac
    57361 agtcgcggga ccggcgaccg aaactaatgt tgttcggcct actcttcggc ctcgtgtcag
    57421 gccttttagt gaacatagcc atcgagtatg cagctcaaca tccaaacgag acgttcagcc
    57481 agtggctacc ggtaaccgcc attcagtgga gtatcgacaa tgtgatcgaa ccggtgggac
    57541 gcgtattctt gcgcttgatt ctaagtgttg tcttgcccct ggttttctcg gcccttgtct
    57601 tggctgtggt ggaatttcgt gacttgcgtc gccttggccg tcttggcctc gctgctattg
    57661 tgatgacggt catattctcg gcggcttctg tcctgatcgg tttaaccctt gtcacggtcg
    57721 cggcaccggg caaggcgatg tccgtggaga agcaacaggc cattgaggct cgttttgggc
    57781 gacgggaatt gccgcgtgaa gaccaagtaa aacgtgtgcc ggatatcgtg cttgatctta
    57841 tcccggaaaa tcctcttcaa gaggctgtgg gagccctgga tgggtcttcc aaaggtaatg
    57901 gtatgctcgc gattatggtc tttgcactcg tccttggggc agcaacaatc aaggcggaaa
    57961 cgcggggtga gccctggatc aaggtcctgg atagcctcta tgctgtttcc atgatcatcg
    58021 tcgattgggc gatgacattg gcaccttttg gcgtagcatg cttgatgttt gtggcagggg
    58081 cccgattagg aattgccctt gtggtaccgt tgttgggttt cctccttgta gtggtgggcg
    58141 ggctgctcct gcaaatgttt gtcgtctatc ccgccgcact gtggatcagt ggtgtccgtc
    58201 cgagccagtt cttccgcaat ctggggcccg tcataccgac ggcctttggt acggcgtcat
    58261 cgagcgttac gctaccgacg gccatccagg tggcagagag aaacctcctc attccatccc
    58321 atattgcccg gtttattctc acggtcggtg caaccgggaa ccaaaacggc acagctttgt
    58381 acgagggagt ggtcgtcctc tttctcgctc aagtatttgg tgcagacttg acaatatggc
    58441 aacaattggg tgtcgttctg tttgccattc tggccggcat cggaaccgca ggcattcctg
    58501 gcggttccat ccccatgatc atgataatcc ttgcgagcat cggtgtgccg ccggagagcg
    58561 tggccctcat cttgggactg gaccgcttcc tcgacatgtg tcggactgtg gtcaacgtca
    58621 cgggtgattt gaccatcgcg cagtgcgtgg cagcctggag cggtcacctt ccaaaaggaa
    58681 agcacgatgg cacaacctag tctcgccggg ccccgcttgc gaggaatgtg gccggacaaa
    58741 cacggtccaa gatgcgatag ccaactcgaa aagctcaatc cctatcgatc cgcagtgcga
    58801 tgtcgtcgtc gaaggctggg gaggtcagcg ttaacaatcc tccatcatgc tgcctggatt
    58861 cgtcaaggac ccatgccccg ctcttggtgt caagccagcg aactctccaa cgtcccgtcg
    58921 gaagatccag tgcaaccttc gccgtgacat tcttgcgaag gtgttccgcc agattcttgg
    58981 gtttgttggg aagcggaaca tgaagataga tggcatagac agcccccttt ttcgacaagc
    59041 aacttgcggt taggtccggt tccacgccct tgattgttcc cgagtccggc ttcatctgcc
    59101 aaaaaggcaa tgcttcgacg aacttcttca gaaccgacaa ctgccgtcga agagtggcac
    59161 tgccaccacc aggagattta taatcaagaa acgttccgtc ggggtaagcc ggggtaaacg
    59221 aataatccaa attgttgtag agtgcacctc cggccaggat aaaatcccat ccttcgctcc
    59281 ggtacagaat atcatctttc ccccggaatc ccgtctcgtt ttccccgatg accacgttcc
    59341 agtgatagtt gagcgcaacg gtgtccggcg gcacacaata gtgaaaattg agaatcttaa
    59401 cggccggatg gagcttttcg actttggcac ggccattcgc aatattcatc gaaatcagat
    59461 gtcggacgcc ccacgttttt tctgtttcga cgatcgtatc agcaattttc cactgccacg
    59521 cctcggtcac accgccaaag tatggttcat tgcacacctc ataatagagg ttgtcgtact
    59581 cccgcagctc ggaaaccact ttccgcacaa agtccagttg cacctcagtc agatcagcgt
    59641 gcttgagtgt ataaacctct tcccgtgggc aggcaccgat gccgttcaca ttgtttttca
    59701 cgttcatggg attggcagcc caaagctcgt cgttgtagaa ggggcaaaac aggttgaatt
    59761 cgacaacgat cccacggtcc cgggccgcct gcatgaaggc gtgtagtcgc gaaaagtatt
    59821 cgggattcca ttttttcaag tcaaaccgat tgccgccgtc ataata
//
LOCUS       DAOSUT010000004        55579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_411_length_55579_cov_9.222931, whole genome shotgun sequence.
ACCESSION   DAOSUT010000004 DAOSUT010000000
VERSION     DAOSUT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 55579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55579
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_55579_cov_9.222931"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1573)
                     /gene="pyk"
                     /locus_tag="PLD05_00715"
     CDS             complement(146..1573)
                     /gene="pyk"
                     /locus_tag="PLD05_00715"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOP75973.1"
                     /translation="MSLRPSQTKIVATLGPASDSYETICSLIRSGVDVFRLNAAHGSL
                     DEHGERLRRIRQAEREIGWPVGVLVDLGGPKIRLGELVGGALECTPGMKVRFVKNDKA
                     ERVSDLTSTYPSLVDELNVGDRVMLADGTVALRVIDKKPDYAECEVIQPGLIRSRQGI
                     NLPGVKLKVAALTEADKEAVAWAMGQEVDFLGLSFVRYPDDIAALRALTSNAPVPPAI
                     IAKIEKPEALEKLEEIVSASDGVMVARGDLGVEVDIAEVAVIQKRIIQVANRLRKPVI
                     VATQMLDSMQHSRLPTRAEVTDVANAILDGADACMLSGETAVGKYPLESVQMMHRIAM
                     ATEATMNRLRYDEEPFEIQEGNPITEAVVKHAGTMATELGAKMLITVTRSGLTTLIIA
                     KHRFPVLTVGISESVAVLRRMSLYRGIVPLRSAPVHDTKQLLDFVLNWARRQDLVQKG
                     DRVIFLFGTGTAPSAHNTVAVYVVD"
     gene            1916..2092
                     /locus_tag="PLD05_00720"
     CDS             1916..2092
                     /locus_tag="PLD05_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOP75974.1"
                     /translation="MGRFKATRRDLLKGSIGAAAAVFVPHVWVTPFSWAESPNERLNV
                     AAIGVGGRGSDIGR"
     gene            2114..3259
                     /locus_tag="PLD05_00725"
     CDS             2114..3259
                     /locus_tag="PLD05_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP75975.1"
                     /translation="MACADVHRLNAENFAKPFEGRCQVYTDYRHVLDRKDVDAVTIGT
                     PDHWHTKIAIDAMKAGKDVYCEKPLTLTIEESKLICEAVRRTGRVFQVGTQQRSEFNS
                     MFLKAVVMARSGRLGKKLKAISSVGQATAGGPFPNTDPPEFLDWDFWLGQAPKVPFCE
                     NRIGWNFRWWFEYSGGQVTDWGVHHTDIAIWALGGEETGPIEVTPVNYEFPLGRELML
                     ETLLGKRRWEDLPVAYNVAKTFDCDMLLPNGNVVKLLSGPNELIIEGELGRIRVNRGS
                     LTGRPIEELTEADKKWLDEEVRKLYRGKEPGSHMRNFFECVKDRSLPISDVFTHCNSV
                     NACHMANIAMLLNRKVRWDPVKYQFIDDPEANALTKRKQREPYTITL"
     gene            3459..4775
                     /locus_tag="PLD05_00730"
     CDS             3459..4775
                     /locus_tag="PLD05_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP75976.1"
                     /translation="MVKHVTTRRDFLATGVAVPMLFLGRYTYGQSANEAIGIGAIGVG
                     GRGSGIGNEAARFGVMLACADVDRSHASRFASRYEGKCEIYEDYRKILDRKDIDVVTI
                     GTPDHWHTKIAVEALRAGKDVYCEKPLTLTIDEGKILRRVVAETKRVFQVGTQQRSDR
                     RFLQAVALARSGRLGKKLVATCSIGAGPQGGPFPTSEPPAELNWDFWLGQTPVAPYTK
                     ERCHGTFRWWLEYSGGKMTDWGAHHVDIAQWGLGCETTGPVEVSGEGNFPTIPENFDP
                     VAFFAGKVRLPNAYNTATTFRCTLKFANGSTIIVQHGPDNGVWFEGENGRIFVNRGRI
                     TGSPIEQLTDAEKAWLEEEVRKLYKGRELRGHMANFFECVRDRGEPISDVASHHRHLS
                     SCHMCNIAMLLKRPLRWDPEKEVFVGDEQANALLSRPQRAPYTIEA"
     gene            4940..5743
                     /locus_tag="PLD05_00735"
     CDS             4940..5743
                     /locus_tag="PLD05_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOP75977.1"
                     /translation="MMWTGKNAVITGGSSGLGLAIARAFARRGARVAIIGLEQHGVEQ
                     ARSELEKHTEVLPLVADIRNEADVIRCRDAIHSAWRTVDVLVNAAGRSMRGAICDTTP
                     EQFRELFELNVVALVQVTRALLPDLLATRGHVVNIGSLASKAAAKYLGAYPATKHAVA
                     AFSQQLRLELGAKGLHVLLVCPGPIARTGPRLYPLEGEERVLEAARAPGGGVKVKAID
                     PDRLANKIVKACEKRLVELVVPGRARILFALAQLFPRIGDWLILRFTPH"
     gene            5924..7237
                     /gene="xylA"
                     /locus_tag="PLD05_00740"
     CDS             5924..7237
                     /gene="xylA"
                     /locus_tag="PLD05_00740"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOP75978.1"
                     /translation="MAAFPEVSKIRYEGPDSRNPLAFRHYNPDELVEGKPMKDHFRFS
                     VAFWHTMRGTGADPFGAATMIRPWDNGQETVENAINRVRAFFEFCEKLGAPFFCFHDR
                     DVAPEGKTLAETNKNLDAVVKVIKEECDRTGIRLLWGTANLFSNPRYVHGAATSCNAD
                     AFAYAAAQVKKALEVTKELGGAGYVFWGGREGYQSFWNTDMKRELDHLAKFLHMAVDY
                     AKEIGFTGQFYIEPKPKEPTKHQYDFDAAACINFLRTYGLEKYFKLNIETNHATLAMH
                     NVEHELEYAGMQGFLGSVDANTGDLLLGWDTDQFPTDVYLTTKIMLVFMKYGGIAPGG
                     INFDAKVRRESFEPIDLFYAHIGGMDCFAKGLKIAAAIRADGELARMVQERYSSWDTG
                     IGAEIEADKHNFKTLEKYMLEKGDVTPNKSGRQELFENIVNRYIF"
     gene            7459..8070
                     /locus_tag="PLD05_00745"
     CDS             7459..8070
                     /locus_tag="PLD05_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOP75979.1"
                     /translation="MKKLNVLVIWGLILGLCVLSTSRGMVAAEEAAAKEEEPKFIRTP
                     DVIYVPTPYDVVRKMLELAEVKKGDVVYDLGCGDGRIVVTAAKEYGVKAVGFDIDPER
                     VKEARENVKKNKVEHLVRIEQKDIFTLDLSEATVVTLYLLPQLNVKLIPQLEKLKPGS
                     RIVSHDFDMAGVIPDKVVNFKSKNGREHTLYLWTTPLKKEKTQ"
     gene            complement(8093..9271)
                     /locus_tag="PLD05_00750"
     CDS             complement(8093..9271)
                     /locus_tag="PLD05_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75980.1"
                     /translation="MKLASIRRFSVLVTGLVLLGCSTNKTGSNFNLARVNPFHWIKPE
                     SETKPYPQKPSSFASPSDATGRYSGTVADSTKGGTLPAAASAQVYPGTQAGYQNVEST
                     TYPGTASMRSGTPYGTTAQSYPATTTTPPSGGYSANPVTATSWQSNPAGVATASDQRN
                     FSQPASSGNTGSMPANPYVPSYSRTTQSATLPSAESSSQVNGSGTNYRPSAATPESKT
                     SQSAFQEATNAGTADWTTLVGERYAQLYQKGSNMPSPSGTDGLGSTGYSPGDTGYVPG
                     KLENPPGNVNYRPGQTGYNPPGVPPYAMPYGTNSQQTNASDRSWPTSTNSAGEYRPGS
                     TKTYIPRTGSQSSNTTVPGTGTNQAATGGLSSFPTTGSLSDQPSNISQTSSVNEPLRM
                     "
     gene            10042..11193
                     /locus_tag="PLD05_00755"
     CDS             10042..11193
                     /locus_tag="PLD05_00755"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOP75981.1"
                     /translation="MIVGVPREIYPEERRVAIVPATVPSLTKAGHVVRVETGAGMAAG
                     FSDEEYRSKGAEVVSRRDVFASEVLLGVRWDIPGEARAETLDLVRPKQVIVGSWDPLS
                     KRENLVAWAEKGVSIFALELLPRITRAQSMDILSSMATLAGYRAVILAAMALPKIFPM
                     LMTAAGTLTPAKVFVVGAGVAGLQAIATARRLGAAVSAYDVRPAVKEQVQSLGAKFIE
                     LDLETSGAEDQRGYAKAMDESFYQKQREMMFRVVAESDVVITTASVPGKKAPVLITRE
                     MVAGMARGSVIVDLAAERGGNCELTQPGKTVIENGVTILGPTNLPSEIPFHASQMFSR
                     NISTFFLHLCKTGSIPFETDDEIIRDTLVVRDGEIVHPMLVGSSAGATA"
     gene            11231..11578
                     /locus_tag="PLD05_00760"
     CDS             11231..11578
                     /locus_tag="PLD05_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOP75982.1"
                     /translation="MLNNCFLLAEQINEVAEVTQGSQLPLALALTIFVLAIFVGFEII
                     TKVPPTLHTPLMSGSNAISGITIVGAIIAAGAGHSGLATLLGTIAVVLATINVVGGFL
                     VTNRMLHMFKRKS"
     gene            11656..13038
                     /locus_tag="PLD05_00765"
     CDS             11656..13038
                     /locus_tag="PLD05_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOP75983.1"
                     /translation="MDRGTVITLAYLLASVLFILGIKGLTHPRTAVRGNLLGALGMLI
                     AIVATLIHWSIIEYHWIILGLVVGAVIGAVLALKVKMTAMPQMVALLNGFGGGASVLV
                     AGAELTRTPPAPYSDALIATYASAVIGAVTFWGSLLAFAKLEEYRAFRQPIRYPLLQP
                     INAVLGLLALALCVIGIGQPQYIGLYWGVVAIASVLGLTLVNPIGGADMPVVIALLNS
                     YSGLAAAATGFVLGNAVLIISGSSVGASGIILTNIMCKAMNRSMTNVLFGVLQADEGA
                     AKQDDVYRNVKGAAAEEIAMLFDTARRVVIVPGYGMAVAQAQHAVRDLYQLLESRGIE
                     VLFGIHPVAGRMPGHMNVLLAEAEIPYDKLKELDEINSILSETDVAIVLGANDVVNPL
                     AKTDPKSPIAGMPIIEVDKAKTVVVIKRSLSPGFANIPNPLFAADNTLMFFADGKRAV
                     LDLIAAIKES"
     gene            13137..>13819
                     /locus_tag="PLD05_00770"
     CDS             13137..>13819
                     /locus_tag="PLD05_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP75984.1"
                     /translation="MRQPLQNCLFCAFLLVFMGNAPLMAESHTVLGVEGSRFTVNGQP
                     TFLLGVSYYGALGVGPEVWKSDLKEIKKRGFNWVRIWANWNAFGQEAFAVDAEGNPYD
                     AGLKRLQAIVAYCDELGLIVDVTLSRGNGVTGPKRLQTLDAHLRAVRVLVTALKDYRN
                     WYLDLSNERNIRDARFTSIEDLVILRKEVRTLDPKRLVTASHAGDISEEELRDYVLKV
                     GVDFISPHRP"
     assembly_gap    13820..13919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14427..15002
                     /locus_tag="PLD05_00775"
     CDS             14427..15002
                     /locus_tag="PLD05_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75985.1"
                     /translation="MLRKLGYLPVLGVLGTIAVFTVGCGTVMSPLDWGVLNYPIPVSP
                     YFQDKLEDKAWEEERYKKMPILPPLVEGAPHVALDEPSDDEVMRALEKARPLEGGLPF
                     LHEVQRSNVRIVKELIADSIDPPRVYPLVGPAQLHHVHWKCTVYFTETTKVGWPIPYT
                     VKNEDAVEVIYIDHDHLHMVGNVDGGPSSNY"
     assembly_gap    15081..15180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15442..16815
                     /locus_tag="PLD05_00780"
     CDS             15442..16815
                     /locus_tag="PLD05_00780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP75986.1"
                     /translation="MADSTRRQFLRSAGTAGIGLTVSVSKSTRRAIGEQSAPVVVPSP
                     NEKPALLGGKPTVTGQWPHWPWVSSSDEEAVLEVVRAGRWYRSRSVEQFEEEYAALNS
                     ARYCIATASGTTALVTSLGALGVGPGDEVIVPPFTFLATVTSVLLHYAMPVFVDSDIN
                     TFLMDAEKLESAITERTKAIIPVHIGGNVANLDRILAIAEKHRLAVVGDACQAHLAEW
                     KGKSAGSWGTTGCYSFQLSKNLSSGEGGAILTNDEAMADACFAFHNCYRKRTRASLEA
                     KYHFGRNANFRMTEFQAALLLNQMKGIKERAQLRSDNAAYLTTLLASIPGIHPAKEYS
                     GCTRNAYHLYMFRYDPNEFNGLPRNRFVNALRAEGVPCSPGYAPLNKEQFLMDALHSE
                     PYVRVYGRQAIEQWPERNQCPENDKLCEQAVWFYQTVLLTDRTRMEQIAAAIQKIKKF
                     AGDLQKS"
     assembly_gap    17106..17205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17206..18527)
                     /locus_tag="PLD05_00785"
     CDS             complement(<17206..18527)
                     /locus_tag="PLD05_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75987.1"
                     /translation="MKIRPLCLGVLAWCLWSVAGRSEDKLPVVIWPVDVHVKVLRDAQ
                     PPEDIQLPLVVKAAQNEYESAQVAVRTAREIKRLTIRASEFRNPENGAILPAESVRMR
                     FVGYIPLKANTPNAERIRVAAAPCEIPDPLLDETEISLAAETSQPIWVTFWIPPGTVP
                     GVYHGELAIVADGNQAKIPVELHVFPFALPDERHLFVTNWFSPGNIAKAHQVEIWSDA
                     YWDILAKYAQNMGQHRQNVVLTPWTLIEVTRNPDGQLTFDYSRFDRFVELFQKMGVAD
                     LIEISHVGHPKGGWGGPVELSNLRAKDKSTGETVSLDYERGLKLLLADLERHLEAKGW
                     LEKAAIHISDEPAIQNVASWRAASELVHRAAPRLRRIDAIETIDFDGALEIWIPKLSQ
                     WDTWRSVFLKRSGQVEFWYYICCHPYGNVYPNRFLDYPTTCVRILHWI"
     gene            18979..19779
                     /locus_tag="PLD05_00790"
     CDS             18979..19779
                     /locus_tag="PLD05_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75988.1"
                     /translation="MTKHGSSRGIRSQVYWLIWAFCVLIPVLAGCNSGPSQEQPKGGE
                     QPAASNSSQNLPQVSQSELQKVTGDPVSAVKVFLEAVRTGDDERVVALFSEEARKQAG
                     QLNRQFAPVGSDTARYEVFEDVQYLAPDGARVRTHWTDLDSRGQPRTDEITWMLRKEA
                     NGWRVAGMATVIFEGEPPLLLDFENMQETLRKVEMLSEEIERRQEAAQKVAAKQEWED
                     AGAENQASGQLPNSPQQDPSAADLPAAPGVNDGSTMTALKPDEPITRE"
     gene            complement(19826..20695)
                     /locus_tag="PLD05_00795"
     CDS             complement(19826..20695)
                     /locus_tag="PLD05_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP75989.1"
                     /translation="MPLTRREMLTSVGGLLASSSLRPLLADQNRLYKIGACDWSIGAR
                     QDIRAFEVAKEIGLDGVQVSFGEPGGSYDLRDQKVRQQYAEAAAKTGVEIASLAMGVL
                     NNIPFSSDPRTVEWVSDCIDVMAAMGQKIVLLAFFGKGDIKGKRDLQDEVIRRLKQLA
                     PKAEEKQVILGVESTLNVDDHLRILDGVGSPAVQVYYDVANMHYAGYDIFAELRRLGR
                     ERICQIHMKEYNHLLGQGPIDFLKIRDVLGEIGYYDWLIIEAATVKGRSVVDCYRDNQ
                     KFLRSLFPTAKTA"
     gene            complement(20999..21238)
                     /locus_tag="PLD05_00800"
     CDS             complement(20999..21238)
                     /locus_tag="PLD05_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75990.1"
                     /translation="MRTFLVICLILSVLAQTGCTGGGAVYQTRDSSMTSYWKGFDPYP
                     EDRTAPAIDGGRPPGFEHPLSEPERVRSKPWLANQ"
     gene            21446..>21895
                     /gene="bfr"
                     /locus_tag="PLD05_00805"
     CDS             21446..>21895
                     /gene="bfr"
                     /locus_tag="PLD05_00805"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOP75991.1"
                     /translation="MKGDEKILKTLNELLKDELTAIVQYVVHAEMCADWGYERLHEAI
                     EKRSRQEMKHAESLIERIIFLEGTPIVSQVGDVHVGPDVQTQLQSDLNAEMGAIQAYN
                     KAIQLAMDLNDSVTRDLLESIAKDEDDHINWIEEQLAQIEQMGLPMYL"
     assembly_gap    21896..21995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21996..23111
                     /locus_tag="PLD05_00810"
     CDS             <21996..23111
                     /locus_tag="PLD05_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP75992.1"
                     /translation="RDRSMSQDKLRRRIAWEAARLIYFREETEYHRARTKAARRICRG
                     EAPLGDLPTNREIREQIEAVARAFEGERRGVEVGEMRLEALRIMQILKEYQPYLVGGT
                     LTGHARRGSPIDIHIFSDSVEAVQDTLQKSHIPFEIVNRPGETAGSEKSCVCFHVTSR
                     FVCRLTLYPAAMVGQLPINVTTGQSIPRANIGELERLIAREHPTLVYRTCPQEEPALV
                     DRFLAYESLLVPLEHIRDDRKGQPDIDLLTHSLQVFHLARSESPYDEEFLLAALLHDV
                     GKAIDPKDHVAAALEALDGYITERTAWLIAHHMEGLQVLDGSIGQRARRRLESSEDYD
                     LLVLLARCDRAGRQVGVAVPDLEDAIEYLRALDRDFG"
     gene            23292..26954
                     /locus_tag="PLD05_00815"
     CDS             23292..26954
                     /locus_tag="PLD05_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOP75993.1"
                     /translation="MNRLKNVTTLIGLVLLAASSLSTVRLRSTFAVEAMHDTQKENAP
                     GVSWHHPLYLHWGGYWKSRVRVTYHNRGARDLAGYPVALPVVSPGSFSAGAASGIPLA
                     GRNIRDIRVCTADGTELLWAACDEDMVSFTEGTFPPKGWLVVPVTCPARGKTEFFVYF
                     DNPEAIEVPDYFSAKPCPINCGVEKGEGETPSGWQHDASGPDRRAYWSNENPHSGAKC
                     LAIEVQPGSKPSWIATRQTGVAVVPGVRYRFRAWVRAENVEGYAGWYIHVGNAENPMM
                     IAPTISGGGGTYDWKQVEAEFTAPEGATTASLGTALWGTGRAWFDDVEWEMDPQACEV
                     VIHSPESSALAEVDETQAWPSSQENHFLGNQRLHLELLNFDTQARQNVLACLGPNVWG
                     GRLPLLAETDRVRMFAGTTRIKPFVVGEYLLWPDHLPARSRVHRYVYCEDPTSVQPIS
                     VQPIEEKDATNKVLLEYMALLNSSNNLVQNFSFEAGGDVPAGWLQSGVGDGRVKFSVE
                     DPKHEGFGRRAARLDIPSGTPSAWRGWQQRIPVKAGTTYLVAGWLKCQDLEGEARIHI
                     HFHDSQGRLCKSGGMTSVGPGVRATQPWTLVSGLVKTPNDAASLTLHLTTNVSGTLWH
                     DAIFVAEVCNVSCVKREAAPMAKDAFVVWQVPAVVKVFREDPPVRERQSIQVSAAKGE
                     WEALQLAVRSGTSLAEVRVEVLPPRNRNNKQLDRFEVTVVGYVPIDYPTNYYRFDGPE
                     WHRKIPQQSPGSDGWPGWWPDPLLPKSQFSLAAETTQAVWIVFKIPQDAESGDYDGTV
                     RFIGQGRVLAEVPFSVHIWNFALPRETTFPAIYDVRLGPAGRVFGQTLDQLYPEIVRF
                     MAERRLCPDNIRPDPVIEYRDGQVRADFTEYDRVARWYFDTLKLPVTYTPHVFYLFGW
                     GFPPRNFFGQTPYPGDSPFEGVNRSELRPEYVAAYQACLKCYWDHMKENGWSDRVVLY
                     ISDEPFDHLPEIREQMIALCKMIHDAVPEIPIYSSTWHHIPDWDGSITVWGLGHDGRV
                     PPEKLRALREGSAQVWFTTDGQMCTDTPYCAVERLLPHYCFHHGVRAYEFWGVAWTTY
                     DPYRFGWHSFIPQSDRPGTRYWVRYPNGDGFLIYPGKPIGYDGLVSSIRLEQAREGVE
                     DYEYLSILKTVATKARPDDPRRKAAESILAKASQLATIPNAGGRYSTKILPNPGMLEE
                     IRKELAEAIEQFVSNQ"
     gene            27089..28138
                     /locus_tag="PLD05_00820"
     CDS             27089..28138
                     /locus_tag="PLD05_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75994.1"
                     /translation="MQTLLERLVFSFGWAKSRILCRRVCEGASVVLVIAMMIHGAVCT
                     LALDCQAEKPRLKPRIEGDWWQVAENPDLGPLTSERQQVVDFGIWQAEDGTWQLWSCI
                     RHTKEVGHTRLFFRWEGRQLTQSPWEPKGIAMRADPDYGEQPGGLQAPFVFRIDKLFH
                     MFYGGWDVICLATSQNGKDFVRKRNEAGKSFLFGPPDGYGNPRDPMVLRVADQWYCYF
                     TAHTPHEGAVFCRTSKDLVNWSEVFVVAKGGQAGSGSYSAECPFVVEASPGEYYLFRT
                     QRYGAKAQTSVYWSNNPLDFGIDNDEGHFLCTLPVAAPEIFRHADSWYIAALMPNLSG
                     LQIARLKWTTDDSMP"
     gene            28399..29304
                     /locus_tag="PLD05_00825"
     CDS             28399..29304
                     /locus_tag="PLD05_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOP75995.1"
                     /translation="MHQYSIDAVLQCLSEGRAEIEATFKRALDQFGSLQLDQISHSSW
                     ADVSGSDWIGQPGLLITFQIKQADVVLALPQSSGCIPDWAYQPDATGKSRLATLAQEL
                     SILIFPPEWPVLTSEANTVTSFEVALEKLMRPDQSQFVSIPFVTPEKKGGLIIGLRCE
                     TATTLTDTSPPNENASQDAKTNASPTERRAMAGDHRSPHANRVLPLLTHSLLRIRLPL
                     EVTLARTRKPLLELLQLCPGAIIQFDKSCEEPLELSINGHVIARGEAVKVGDKFGLRI
                     LEMTPPPERYIVLGPRTAKKEIKQK"
     gene            29646..30248
                     /locus_tag="PLD05_00830"
     CDS             29646..30248
                     /locus_tag="PLD05_00830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOP75996.1"
                     /translation="MIFILDNYDSFTYNLVQRLGELDNSLEIVVRRNDQVTVEEIERM
                     SPDRIIISPGPCTPKEAGISVECVRYFAGRVPLLGVCLGHQSIGEAFGASIVRAKRLM
                     HGKTDTIFHNGCELFEGLPNPFIATRYHSLVIDPPSLPDCFEVCSWSFLPDGEKEIMG
                     IRHKTFPLYGLQFHPESFLTEHGPTILANFLKLPSLAGRS"
     gene            30413..>30858
                     /locus_tag="PLD05_00835"
     CDS             30413..>30858
                     /locus_tag="PLD05_00835"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75997.1"
                     /translation="MSQPIILAIAGAALLWAALPPLDWWPLAWIAAVPWLILIDSPKT
                     WTRRQYIQWGIVGFFFWMAALHWLRLPHWATSFGWVALSFYLGWYLPAFIGLSRIGVR
                     LLRLPLNVVAATVWAGLEVVRAHLLTGFTMASLSHTQYRWISLLQIA"
     assembly_gap    30859..30958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30959..32126
                     /locus_tag="PLD05_00840"
     CDS             <30959..32126
                     /locus_tag="PLD05_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOP75998.1"
                     /translation="TLLYGQWRLSQFKTGQVNRSVRVALIQGSEDIRLEDSEEKRERI
                     HRHYLDLAFQAARSHRDLDLMVWPETVYGGFLIDHDDSPAVPATWADSDMTPERFREQ
                     IGKAREHSQRSLETLAQHLRCPLLIGVETVSYTSQGPRIFNSAAYVALCEKINASQGG
                     RERFSDGTDVKEANEESGKKGDSTGQRYCYFGRYDKMHLVMFGEYVPFANVIPLLQRL
                     TPLSTSTTPGKIPVAFPVGSLLLSPNICYESVIPHLIRNQILTLRAQGQEPDILVNLT
                     NDGWFWGSSELDMHLICGVFRALETRKPFLIAANTGFSAHIGPDGRIYQQGPRRDVAI
                     LVADVTRSPLRSLYLDYGIIPNTLLVSFIVLLVIAKCLSASRTFQRKITFRRSA"
     gene            complement(32104..33195)
                     /locus_tag="PLD05_00845"
     CDS             complement(32104..33195)
                     /locus_tag="PLD05_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP75999.1"
                     /translation="MKTRTGLNQSVSQNLRVVFQALLGLIGFFSLLCDVARAQAIEPR
                     IQLEVAVDPRLPPLTHHDWAKALSEVGVENLRVRTAYPNDTPNIQEIPSESVPTYQVL
                     GVVNAQNELLLPDARFTIRQIDRLKQWINDLRHQGPPQSRPKQVAFGLNIVQFEQVRQ
                     DMSRPVQASTKGRPRAELVETLVSSLSYPTVFDPQARQRLGRDLWEEELQGLTTGTAL
                     AYILRYDGLALVPQSTVNGIRYQIVIGHPNLTAWPVGWAPEESEAKVVPKLLETLTVN
                     IQDVPVKDVLQSVTQRLSLMYLLDHPALARFGIQIDKALVNIPQTKTTYSQIIKRSLF
                     QGRLKGEIRCDEAGKPFLWITTLKPIDET"
     gene            complement(33192..34613)
                     /locus_tag="PLD05_00850"
     CDS             complement(33192..34613)
                     /locus_tag="PLD05_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOP76000.1"
                     /translation="MSLFEKEDEQRRREVQPLAARMRPRTLDEFVGQEHFLGEGKILR
                     RLIRADRLGSVIFYGPPGTGKTTLARILATETKSVFQQISAVTSGVKELREIIDGAAR
                     RLLASGQKTVLFIDEIHRFNRAQQDVLLPHVEEGGLVFVGATTENPFFSMTSALVSRS
                     RVFEFLPLTPEHIKVIVRRALTDRERGLGNYEVIMEEEALEFLAQACEGDARRALSAL
                     EVAVLSSETFPLRLTRQLMEDSLQRKAIVYDRQGDAHYDTISALIKSIRGSDPDAATY
                     WLAKMLEAGEDVRFIARRLMILASEDIGNADPTALPLAVAAAHACEMIGLPECRLNLA
                     QVVIYLACAPKSNAVIKAIDEALEDIRSGRVLPVPVHLRDANYPGAKRLGHGQGYVYS
                     QEEPDAIAEQDYLGVDKTYYVPSDRGFEKELKARLEVIRERLGRANKKISDLPDASTS
                     SLPEGSSSPPRLRKARKTKENQS"
     gene            complement(34726..35403)
                     /locus_tag="PLD05_00855"
     CDS             complement(34726..35403)
                     /locus_tag="PLD05_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOP76001.1"
                     /translation="MSPMPSLKEIYDRLYNAFGPQNWWPGETPFEVLVGAILVQNTAW
                     ANVEKAIRNLREEGLMCPKRLYRLKDAELEELIRPCGYYRIKARRLKNVLRYLLKRHD
                     GSLEQMFRLPIDQLRRELLEINGIGPETADSIILYAGNLPTFVVDAYTHRIFSRHGWV
                     DPEADYHTLKEFFESQLEPDAALYNEYHALLVRLGKDYCKKRPRCQACPLEIYLPDGQ
                     PYDAWLE"
     gene            complement(35566..40083)
                     /locus_tag="PLD05_00860"
     CDS             complement(35566..40083)
                     /locus_tag="PLD05_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOP76002.1"
                     /translation="MSRFALVRGDGCASGTGGRTTLRTKSHNHPSAPGRCVRKGCSRK
                     SNNLRIAVEQLESRELLSVTPLDPLLSMGLAETGPTTPSSTVSPIHIDWTTFSQTETG
                     YWRAYGRVAVSGIPQVQAVGAGNIVVLDGSDLWLSTGDPVVPVSSSLLVLPKGTQIAN
                     VRIAATGNQVVLASGAELVVAPNPVPFDSDELPGALWTEVLDRSLTLENTVHFKTFSW
                     GGYSLASVAVTPVVYDQATSTLSYYEEIEFELSLQNNIAGETLPVVDQQLASQLAGLV
                     ANPEVLQTYQILTPTSTDLYEYVVITNASLSSEFLPLVQDKVRRGITARLVTTEWIAQ
                     NYTGTETGDLADRIRDFIRDSYLRHGTRWVLLAGDSEIIPARGVHVTVGNISDSNLAT
                     DMYYACLDGPWNADGDNLWGEPTDGLGGADIDLVPEVFVGRAPVSSVVEARNFVTKTL
                     TYTNVAHPNLESALLLGEKLDSITQGSTSNEIIRQQAIPSNWNITTLYDGATSAWTTS
                     QVIASLNASPNIVHHLGHANSGYVARMTTSQVALLQNAFPYIMYSQGCDAGSFDTRDI
                     AVAEQHVIAPAGAVAVIMNTRYGWYVPGTSPGGSHDYALAFFKAIFNEHKTRLGEAFI
                     ESKIDNLFRLTAGGAYRWIHLSCTLFGDPELVIQTHDWQPPGNSQIAGYVYQDLNANG
                     SFDIGEPGVAGAIVYLDLDQDGRRDQGATTFTQSTPQTLVDYGTTLSRLTVSGVGKVH
                     TLLVSVNITHTYDADLLLTLISPSGRRVRLASNVGGSGDNFVGTVFDDSAPQLIGQGV
                     APFTGSFRPEEPLSLFTGEDADGVWTLEVRDTMYWDTGVLNDWSLTFVYDEPYVVTQA
                     DGSYVFSGLPAGTYTVCYELPSQESLPPFANTGRTVSVARNQTVTGVNFASPSASSII
                     NLGYVTDIIVQIGGQPQSLYRFSTRHEGIVSLLLASSPEAAGGQVFVYSAQGRLLSGE
                     MTATTNGRWDWQVGANEDYFLVFSGFQGDQRFRLVNLLGISDDGLAIHGTEGADQVTV
                     LLTDGVSVTVNGVSYDFSATELGTRRTIYIDTYDGYDNIAIQLPRGDATLTSSPGYAT
                     VSVEGWQVRANNSETIRVRAGSGTSVAHMSDSPGSDLFICRPGYAEFSGNGFLTVVEN
                     FSIVHGYSRLGGDDIAYLYDSAGDDRFVARPEQGVMSGEGYYHRAKGFRYCHGYSTAG
                     GYDTAQLFGSAGNDTLVGRPDWTSLRGENYFLRAKFFELVQASGGNGGRDAATLTGSE
                     GIDTFVAGPRWADMTMPSTQIRIQKFASITANASGGWDTALLYDSPGDDHLRVWPRKA
                     EMTGSGYQINLLNFEEITGKSSAGGTDIAELWDSPGNDEFVSQPRESYLSGVGFINRV
                     YNFATVHGYSTAGGIDVAYLDGGPNINAWVVRPDQTTVSSEDSYRRAKAFERVFALGG
                     LDLCDTARLLDSTGNDELVIDSDQIVFRYPTSVVTLSRIPKVTARTTGGIDRVVGRAV
                     DVVLTLQGLWL"
     gene            complement(40510..41529)
                     /locus_tag="PLD05_00865"
     CDS             complement(40510..41529)
                     /locus_tag="PLD05_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76003.1"
                     /translation="MSTLGRFTALIVVFLFFAILVEGGKFYSPRNLENILRQSAVYAT
                     ASLGMTMVIIAAGIDLSIGSIIALSLVTVAWILDYHYLVPTADGGMETVYLIRVYPVV
                     LPWIALLAGIGVGTLAGFVNGVTIVRLRLVPFIVTLGTMGIFRGLAKGLAEEKDIYPH
                     EDTWLTQLMDPTLTSQDVHWRMWILPPGVWILIATAIFAAVLLRYTRLGRYMYAIGSN
                     EETARLCGVPVGRVKVVTYTLAGFFGGLAGLMQFSFIGGIGQPTSAVAYELFVIAAVV
                     IGGGSLMGGQGSILGSVIGALIITILYMGGQQMGWPKWVQEAVIGVIIIAAVVIDRLR
                     QQKTG"
     gene            complement(41592..43100)
                     /locus_tag="PLD05_00870"
     CDS             complement(41592..43100)
                     /locus_tag="PLD05_00870"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOP76004.1"
                     /translation="MDGICKRFGATIALENVSLEVLPGEVHALVGENGAGKSTLMKIL
                     SGAIPPDRGTMWLDGEPFAPLHPLDARRHGIAMIYQELSLAPHLSVMENILLGMEPHR
                     GGFIRWGQMKRRAEEALSQLGLSDLPLSVPVRALPPGLQQMVEIARALVTQARVVVLD
                     EPTSSLTQDDVVRLFDLVKRLRERGLSVIYISHFLEEVKQISDRFTVLRDGHSVGTGS
                     TASTPVDQIVRMMVGREITELYPRRTRTRGEVVLEIRGLTGIDKPRDVSLTLHRGEIV
                     GIAGLIGSGRTELLRAIFGLQPITQGLVRVGVLSGWADPGKRWRQGVGFMSEDRKREG
                     LAMGLSISANMTLPRLDYLGPWGLILPSRERQMAERWIQSLAIKCQGPGQPVWALSGG
                     TQQKVALSRLLYANVDVLILDEPTRGIDVGSKAQIYRLIAQAAEGSPEYGTRPRAVLM
                     VSSYLPELLGLCDRIAVMYRGQLVACAPVGDWTEHKLMLAATGQTSELAEHQ"
     gene            complement(43299..44312)
                     /locus_tag="PLD05_00875"
     CDS             complement(43299..44312)
                     /locus_tag="PLD05_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOP76005.1"
                     /translation="MQLRTYTHGMLVSFLILTSLSLSCRSQTGEGIAREKKLTIAVIP
                     KSTGAEFWETVEEGAREAAERLNVQMKWEGPLTETELAEQNKIIENMISLQVDGIALA
                     PLNRTAQRRTVERAVQAGIPVVIFDSEIDGDAHVSFVATNNKQGGVLAAQHLAELLGE
                     KKGRVMVLRFVQGTGSTEARAEGFIETAKAAGFEIVADPYPEDATVAGCKKTAANTLE
                     GFVKNNELQLDGIFACNDRSTLGMLAALDDLRKSGVKVNAKFIGFDFTPRLVEAIQQG
                     DIDALIAQNPRKMGRLAVETLVSYLRGQQVPKYIDTGVEVVTRSRLESDPAIRQLVGL
                     KDK"
     gene            complement(45344..46765)
                     /locus_tag="PLD05_00880"
     CDS             complement(45344..46765)
                     /locus_tag="PLD05_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP76006.1"
                     /translation="MGHQYLALKVLLLFWAGGLSLFAQTAESSENARSKLPNFVIILA
                     DDLGYADVGFQGCQDIPTPNLDTLAREGMRCTQGYVSHPFCSPTRAGLMTARYQQRFG
                     HENNPKYDPNDPVAGLPISQVTVADVLSRAGYVTGAVGKWHLGAAPHFHPLRRGFHEY
                     FGLIGGGHDYFRVGPPGENREYFIPLQRNTEPEAFEGYLTDVLTSEAVAFIRKHHQVP
                     FFLYLAYNAPHTPLQAPESWLQRVSHIEDPTRRSYAAMVAALDEGVGRVCAALKELGL
                     EERTLVFFLSDNGGPVEVTHASNAPLRGAKGSVYEGGIRVPFVIRWTGTLPSGTTYEQ
                     PVISLDIPATIVAVADTAFPTESAVDGVNLLPYLQGQRQDQPHKSLFWRTGGGTNWAV
                     RSGDWKLVKVGQQAEELYNIGEDISETKNVSHQHPEIVLEIRRQYETWNQKNIAPIFE
                     GPRRARPAGNKLQKDANRASAQP"
     gene            47111..48523
                     /locus_tag="PLD05_00885"
     CDS             47111..48523
                     /locus_tag="PLD05_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOP76007.1"
                     /translation="MKLSLWHHLWRPITRQILGSMILISVLSSLIASLGMGLIWSAQI
                     KNRYREELRRVCEIIKHGGFPLHRTVLAQLSGLSGMEFISLSTRGAVIDSTLKLSNED
                     EGLLAALVAKHGNADSSSHPRSLNLGGTHYQIVIVSVLAARGGAAPIDRVIVLQEETA
                     LLRELQQVVLPTLLATVAAVVIGFLLSIGIARHISQPLVSLTEQTIKAAHDLQSDIAL
                     PKTDDETHALAEAIQRLITERRGYEQSVRQEARLQAAHQFALGLGHQLRNLLTALRMA
                     IELHAERCHVNRNDEDLLVALRQVRLIDSVLRQFLSLRAFAELEKEPLDYAQVLESVL
                     DLLRPAFQHAGVQLRVEMPQKPCVVTGHRASLEQLTTNLVMNALEAVQGVRDEPVVTV
                     ALRRSEEGVIQLVVEDNGPGPPASVAKDLLKKPVTSKSDGIGLGLFVAQLIAEQHNGR
                     IIWRRENGRTQFVFELPNHS"
     gene            48568..49998
                     /locus_tag="PLD05_00890"
     CDS             48568..49998
                     /locus_tag="PLD05_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP76008.1"
                     /translation="MGHILIVDDQPDICWGLKRIAEQLGHTAESVATAEEALSRVLST
                     NPDVVVMDVRLPGMTGLDAMQELKKKGWEGPVVVITAYGDVEVAVEAVRQGAFEYLTK
                     PFDLKVVARAIERAVASRLSTPTDEKTGELEERSWPQIVGVSPAMQQVFKQIAMVAPT
                     DAAVHIVGESGTGKELVARSIHGFSHRRKGPLVAAHIAALSPSLAESELFGHVRGAFT
                     GADYDHVGLLQRAHGGTLFIDEVAEIPLNLQVKLLRAIEYQEVTPVGAVRPIPCDFRI
                     ISATHRDLRKLVAEGKFRHDLYYRLITFEIRVPPLRERPEDIEPLTKFFLKELCRRME
                     KPVPHWTPEFMAALLSRPWWGNVRELRSALEHALILCRGGLLHPDLLPPPLPQASSQV
                     TAVASNLQDCVRQWTQDVLKTGQAVGHIHQEFLDIVEPVLFETVLQHYGGQYVNAARA
                     LGIHRVTLRRKMDRVKRSESGQQWFR"
     gene            50145..50684
                     /locus_tag="PLD05_00895"
     CDS             50145..50684
                     /locus_tag="PLD05_00895"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOP76009.1"
                     /translation="MSSAAEIELKPFIRSIPDFPKPGILFRDITPLLASPQAMKATIR
                     YLSEAVRNRDVQMIAAAEARGFLFAAPLAIELNVGLVPIRKPGKLPSDTISHSYTLEY
                     GTDTLEMHKDAIAPGTRVLVVDDLLATGGTVEACCRLIEKAGGIVVGCAFVIELTDLK
                     GAERLARYHPVSLIKFSGK"
     gene            complement(50692..52155)
                     /locus_tag="PLD05_00900"
     CDS             complement(50692..52155)
                     /locus_tag="PLD05_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76010.1"
                     /translation="MTPIKTPRLAIGTIDEETDPWSFLRIFLQELTSQGLHVQTFLSQ
                     ASFFGFQNLKRWSGATPRRLDSWLMSPARCCESFIRGVVGADLAVVVGEYERGGGCHF
                     GTGQHTTDERASFRNVRGPCRFQAEKGSAKVISQGRSRSVCGAPDATSFFGHLGTLCD
                     WLSLSKLVILRGQGYHEFLGEGPLSEKAMDGVVVVDADPEIAPLQYLLDFEVYCGLPL
                     VGILYPRPPEKCDPIKGFSVWADEPAQLWWNDHQFQRILFRSAQRPFTIEEWPVLDYR
                     GRITVALARDAVFGCYFPETLESLERCGARIVDFSPVKDERIPEDTDVIYLGCGQPEP
                     YAEDLANNHCLKAAIRNHVRRGGRIYAEGAGAAYLCQWMEIQPGQFVRGVGLFPAAGR
                     QLPRFGEPLPVTVTVARPMWLASRGTVLRGYRSPKWWFEPEPLLAPAIAEPGFEYDII
                     GNDRVVASVIHLDFGTHPDLLASFFAAKPESRLLPNP"
     gene            complement(52344..52991)
                     /locus_tag="PLD05_00905"
     CDS             complement(52344..52991)
                     /locus_tag="PLD05_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOP76011.1"
                     /translation="MGSNNFAWQGNMVAATLRVILALTARVLSGASVRWVDCQPDTCQ
                     RVYFANHTSHLDAVVIWAALPPQVRAVTRPIAAKDYWTANPVRRYLSVEVFNALLIDR
                     QEIKVHHSPVSDIVSAMGDKYSIIIFPEGGRSSGPEVGPFKSGLYYLAKRRPDLELVP
                     VFLDNMNRILPRGEFLPVPLLTRVIFGPPMWLESGEPKHLFLERARQAILRLKEV"
     gene            53470..54459
                     /gene="galE"
                     /locus_tag="PLD05_00910"
     CDS             53470..54459
                     /gene="galE"
                     /locus_tag="PLD05_00910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOP76012.1"
                     /translation="MQVLVVGGAGYIGSHAVKMLREQGHTPIVVDNLTRGHRKAVPSD
                     IPFYQISACEIERLCEILKKHSIECVMHFAALAYVGESVEKPLMYYENNVSATIRLLQ
                     AMETVGVQKFVFSSTCATYGEPSTIPITEACPQKPINPYGRSKLMVEQILADYAASHP
                     AFAYAALRYFNVAGASRDGTLGEDHRPETHLIPSILLTLLGRRDKFVVFGTDYPTPDG
                     TCIRDYIHVEDLCAAHILVMEKLCPGQPRIYNLGIGRGYSVKEVIDAVERVTGKRVPV
                     EHGPRRAGDPAILYADATRIRKELNWKPQITTIEEMVETAWRWHSAHPEGYVS"
     gene            54520..55455
                     /locus_tag="PLD05_00915"
     CDS             54520..55455
                     /locus_tag="PLD05_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOP76013.1"
                     /translation="MKATIALVFAVLGSVIVYPVLVYGDEPPKSIEEIVAPGTEPQVI
                     GSGYGFSEGPAADGEGNVYFSDGKNDSIYVYRVGQGVELFVNDSTDANGMMFNSKGEL
                     YVCEGAARRIVAFDVKTKKKRVLASEFQGTAFNEPNDLTIDQQDGFYFTDPNYRHRGQ
                     PSLMKEDVYYVRADGTVSRVSQVCIKPNGILLTPDNKVLYLADNAAGIIYRYDVVGPG
                     QLAHETKWIELGAHPDGMTLDEFGNLYVACGRAGIKVYTKNGEYLGTISVPYASNCVF
                     GGKDFRTLFVTSADKVYAIPTKVRGILPLVLRKQE"
BASE COUNT        12654 a        14632 c        15106 g        12687 t
ORIGIN      
        1 agaatctccc ctctcccggt cagcgggaga ggggtcgggg gtgagggtgg ctcgagtcgc
       61 tgccggtttt ctcgatggac tctgacggtt ggttgccctc accccaaccc tctcccagag
      121 ggagagggaa ctttgggata gccttttagt ccaccacata cacagcaacc gtgttgtgcg
      181 cgcttggagc cgtccctgtt ccaaaaagaa agatgacgcg atcacccttt tgaacgaggt
      241 cctggcggcg tgcccagtta aggacaaagt cgagcaattg ctttgtatcg tgaacggggg
      301 cgctgcggag cggcacaatt ccccggtaca ggctcatgcg cctgagaaca gctacactct
      361 cgcttattcc gacagtaagc acagggaagc gatgcttcgc tatgatgagg gtggtgaggc
      421 ctgaacgagt cacggttatg agcattttcg ctcccaattc tgtggccatg gttccagcgt
      481 gtttgacaac cgcctccgta atagggttac cctcttgaat ctcaaagggc tcctcatcgt
      541 atcgaaggcg attcatcgtg gcctctgtgg ccatggcaat gcggtgcatc atttggaccg
      601 attcgagcgg gtactttccc accgccgtct ccccagatag catgcaggcg tccgctccat
      661 caaggattgc atttgccaca tcggtcacct cggcacgcgt tggcagccgt gagtgttgca
      721 tgctatcgag catctgggta gccacgatga cgggctttcg gagccgattg gccacttgga
      781 ttatcctctt ctggatcacg gctacttcgg ctatgtccac ctcaaccccc agatctcccc
      841 ttgctaccat gactccatcg gacgctgaca cgatctcttc gagcttttcc aaggcctccg
      901 gcttctcaat cttggcgatg attgccgggg gtacaggtgc gtttgaggtc agagcgcgaa
      961 gggctgcgat atcatcgggg tatcgcacaa agctcaaccc taaaaagtcc acctcttggc
     1021 ccattgccca agccacggcc tctttgtcgg cctcggtcaa ggccgccacc ttcagtttaa
     1081 cacccggtaa attgattccc tgacggctcc ggatgaggcc cggctggatc acctcacatt
     1141 cggcgtagtc tggttttttg tcgataactc ttaaggcgac cgtgccatcc gccaacatga
     1201 cgcggtcacc cacgttgagt tcatccacaa gggaaggata cgtgcttgtc aaatcggaca
     1261 cccgctcggc cttgtcattc ttgacgaagc gaaccttcat gcccggcgtg cattcgagcg
     1321 ccccgccgac aagttcgccc agccggatct ttggaccgcc aagatcgacc aggacgccga
     1381 ccggccaacc aatctcccgc tctgcttggc gaatacgacg cagccgctct ccgtgctcgt
     1441 cgaggctccc atgggcagca ttaagacgaa agacgtcaac ccctgagcgg atgaggctac
     1501 aaatggtctc gtaggaatca cttgctggtc ccaaggtggc cacaatcttt gtttggctgg
     1561 ggcgaaggga catggctaga gctcccattt gttttcccat ttctttattt gcaatccacg
     1621 gtactttgtg atggcgcttg acagggcttt tcgaattccg gcacccagag ttcgccgtag
     1681 tattgcgagg acggcgccga acagtattct tcttttttat gttctcgatc ggtggcccgc
     1741 gttcatcgca ccttggagaa accagttcat cggggtaacc gagcacaacc ttatgataca
     1801 aactttttgc cggacaagtt gagaatcgca acggatggga ttattgttat aatttggttt
     1861 ttgcaattgc ctttccgcaa cggttttctt aagccccaat agtgaggagg tttcgatggg
     1921 tcgtttcaag gcgacacggc gtgatctttt gaaaggtagt atcggtgcag cggcggccgt
     1981 ctttgtgccg catgtatggg tgacaccgtt ttcttgggcc gagtcaccga acgagcgact
     2041 taacgtcgct gccattggtg tgggtggtcg cggaagcgac atcgggcgct aggccgcaca
     2101 gttggggaac ttggtggcat gcgccgatgt ccatcgtctg aacgctgaga attttgccaa
     2161 gccatttgaa gggcgatgtc aagtttatac ggattatcgc cacgtgctgg atcgcaagga
     2221 tgtcgatgcc gtaaccattg gcacgcccga ccactggcac acgaagattg ccatcgacgc
     2281 catgaaggcg ggcaaggacg tttattgcga aaagcccctg accctcacca tcgaagagag
     2341 caagctcatt tgcgaggccg ttcgccgcac tggccgcgtt tttcaggttg gaacacagca
     2401 gcgaagtgaa ttcaactcga tgttcctgaa agccgttgtc atggcgcgaa gtgggcgttt
     2461 ggggaagaag ctgaaggcca tcagttcggt aggccaggca acagcgggtg gacctttccc
     2521 caacacggat ccaccggagt tcctcgactg ggatttttgg ctcggccaag ctcccaaggt
     2581 gcccttctgc gagaatcgca tcgggtggaa tttccgctgg tggttcgagt actcaggcgg
     2641 acaagtcacc gattgggggg tgcaccacac ggacatcgcg atttgggcat taggtggcga
     2701 agaaaccggc cccatcgaag tgacgccggt taattatgag tttccgctcg gtcgtgaatt
     2761 gatgctggag acgcttctag gaaagcgacg ctgggaggat ttgccggttg cctacaacgt
     2821 tgcgaaaacg ttcgactgcg acatgctcct ccccaatggc aacgtcgtta agctacttag
     2881 tgggccaaat gaacttatca ttgagggtga actcggacgc attcgtgtta atcgggggag
     2941 cctcaccggc cgtcctattg aagagctcac cgaggccgac aagaagtggc tggacgagga
     3001 ggtccggaag ctctatcgtg gcaaggagcc gggcagccac atgagaaact tcttcgaatg
     3061 cgttaaggac cgtagtttac cgatttcgga tgttttcacc cattgcaact cggtaaacgc
     3121 atgccacatg gccaatattg cgatgcttct gaatcgcaag gtccgctggg atcccgtcaa
     3181 gtaccagttc attgacgatc cagaagcgaa cgccttgact aagcgaaagc aacgagagcc
     3241 atatacgatt acgctttaag aatgacttag ggaatggccc aagctgtatc ctcgaggtga
     3301 cttgtaaccg ggcgcggcgg aggtaatgtg acgccgtcgc gccctttttc gacatatctc
     3361 caatcattgc tcgcggcaat cccattcgct agaatgcgaa tgttccgatt tcgctttccc
     3421 ctggtttgcc agccatttcg ggtcttagga gaagcttcat ggtcaagcac gtgacgacac
     3481 gacgcgattt tcttgcaaca ggtgtagcgg ttcccatgct ctttcttggc cgatatacct
     3541 atggtcagtc ggccaacgag gcgattggga tcggcgccat cggtgtcggt ggacgaggat
     3601 cagggattgg gaacgaggcc gcccgttttg gcgtgatgct ggcttgcgca gacgttgacc
     3661 gtagccatgc aagcagattt gcgtcacgtt acgagggcaa atgcgagatt tacgaagatt
     3721 accgaaagat tctggatcga aaggatatcg atgttgttac catcggaact ccggaccact
     3781 ggcacacgaa aattgccgtg gaggcgttgc gggccggaaa ggatgtttat tgcgagaaac
     3841 ctctcacgct gacgattgac gagggaaaga ttctcagacg agttgtggca gagactaagc
     3901 gggtctttca agttgggacg cagcagcgaa gcgatcgccg gttcctacag gccgttgcct
     3961 tggcccgcag tggtcgtttg gggaagaaac tggttgccac ctgttcgatc ggcgccggtc
     4021 cgcaaggtgg cccattcccg accagtgagc cgccggccga gcttaattgg gatttttggc
     4081 tcgggcaaac gccagtagca ccctacacca aggaacgatg ccatgggacg ttccgttggt
     4141 ggcttgaata ttctggtggg aagatgacgg actggggggc ccaccatgtc gatatcgctc
     4201 agtggggtct aggttgtgag accacgggtc ctgtggaagt aagcggcgag ggcaatttcc
     4261 ccaccattcc cgaaaacttt gatcctgtgg ccttttttgc cggaaaagtg cggcttccta
     4321 atgcctacaa cacggcgacc acattccgtt gcacgttgaa gttcgccaat ggtagcacga
     4381 tcatcgtcca gcacggcccc gacaacgggg tatggttcga gggggaaaat ggccgaattt
     4441 tcgttaaccg tggaagaatt acgggcagcc caattgaaca gttgacagat gccgagaagg
     4501 cctggttgga agaggaagtc cgcaagttgt acaagggacg ggagctgcgt ggccatatgg
     4561 caaacttctt tgaatgcgtg cgggatcgcg gcgaaccgat ctcggatgtc gcaagccatc
     4621 atcggcacct cagcagttgc cacatgtgca atatcgctat gctcctgaaa aggccgttgc
     4681 gatgggatcc tgaaaaggag gtgtttgtcg gcgacgaaca agcaaatgct ctcctcagcc
     4741 ggccgcaacg ggcaccgtac acgattgagg cgtaagccat ttagttccac gtgctgaggt
     4801 aggaccgaag ccccacctag acatggggtg attggggtgg tggaatcaaa gtcagtactc
     4861 cgtgctcgaa cgggtactgg cttttttgtt tggtctttca gggacgattt gcacacagtc
     4921 aagaggaaag agcgatgata tgatgtggac tggcaaaaat gcagtcatta caggaggctc
     4981 gagcgggttg gggttggcaa tcgcgcgggc atttgcccga cgtggcgcga gggtagccat
     5041 tatcggcctc gaacaacacg gcgtggagca agctcggagc gaattagaaa aacacacgga
     5101 agtcctgcca cttgttgcgg acatacggaa tgaagccgat gtcattcgct gccgcgatgc
     5161 catccattcg gcctggcgaa cggtcgatgt attggtaaat gctgccgggc gttcgatgcg
     5221 gggcgcaatt tgcgatacaa caccggaaca gtttcgcgaa ctctttgagt tgaacgttgt
     5281 agcccttgtg caagtaacac gcgccttgtt gcccgatctt ttggctaccc gcgggcacgt
     5341 cgttaatata gggtccctgg catccaaagc agcggcgaaa tatttgggtg cctatcccgc
     5401 aacaaaacac gccgttgcgg cgttcagtca acaacttcgc ctggagttgg gggcaaaagg
     5461 gctgcacgtc ttgctagtct gtcccggtcc catcgcgcgg actggcccaa gactctatcc
     5521 cctggagggt gaagaacgag tcctcgaggc cgcccgggcg ccaggtggtg gcgtcaaagt
     5581 taaggcaatt gatcctgaca gacttgccaa taaaatcgtc aaggcttgtg aaaagcgatt
     5641 ggtcgagctg gttgttccgg gtcgcgcgcg gattcttttt gcattagccc aactatttcc
     5701 ccggataggc gattggctca ttcttcgatt taccccacat taagtgagat tctcatgcga
     5761 ccagcgagtc tttctctcga tggaaaacgt ctcgaacacc cctcttgcga tcgatgccgc
     5821 aatgtgtcac catagtaggc cacactgagt gtgtgattaa agaactccgg caagaggaag
     5881 aagttgcgtc aagaaaaagc cgtgtcatca aggagattga accatggctg cttttcccga
     5941 agtgtcgaag attcgttatg aaggtccaga ctctcggaac ccgttggctt tccgacacta
     6001 caatccggat gaacttgtgg aaggaaagcc aatgaaagac catttccgct ttagtgtggc
     6061 cttttggcac acaatgcggg gaacaggggc cgatcctttt ggagcagcaa cgatgattcg
     6121 cccctgggac aatggccagg agaccgtcga aaatgccatc aaccgcgtcc gggcgttctt
     6181 tgaattctgc gagaagttag gggccccgtt cttttgtttc catgatcgcg acgtagcgcc
     6241 ggaaggaaag accctcgccg agacgaacaa gaatttggat gcagtggtta aggtcatcaa
     6301 agaagaatgc gaccgcacgg gcatccgttt gctttgggga acagccaacc tgttcagtaa
     6361 tccccggtat gttcacggag ctgccaccag ttgcaacgcc gatgctttcg cttatgccgc
     6421 ggcgcaggtg aaaaaggcct tggaagttac caaggagttg ggtggggccg gttacgtgtt
     6481 ctggggtggt cgagaagggt atcagagttt ctggaacacc gacatgaaac gggaactcga
     6541 tcacctggcc aaattcctcc acatggcagt ggactatgcc aaggagatcg gttttaccgg
     6601 ccagttctac atcgagccga aacccaagga accgacgaag catcagtatg atttcgatgc
     6661 ggcagcgtgc attaatttcc tgcggaccta cgggctcgaa aagtacttca agctcaacat
     6721 cgagacgaac cacgcaacat tggccatgca caacgtcgaa cacgagcttg aatacgccgg
     6781 catgcaaggc tttctcggct ctgtggatgc caacacgggt gatttgctcc tcggttggga
     6841 caccgatcaa tttcccaccg atgtttacct gacaaccaaa attatgttgg tcttcatgaa
     6901 gtatggtggg attgcaccgg gcggaatcaa cttcgacgct aaggtccgtc gagaaagctt
     6961 cgagcccatt gatcttttct acgcccacat cggaggaatg gactgctttg ccaagggcct
     7021 taagattgcg gcggcaatcc gggccgatgg cgagctcgcc cgcatggtcc aagaacgata
     7081 cagctcgtgg gataccggca ttggggctga aatcgaggcc gacaaacaca acttcaagac
     7141 cctcgagaaa tacatgctag aaaagggtga cgtgacgccc aataagagtg gccgtcaaga
     7201 attgtttgag aacatcgtga atcgttatat cttctgactt gcgtgatcgg tgttccgatc
     7261 gtgtgaggtg ttgtttccag accggtccag ggaggccggt ctttttgttt tccaaacagc
     7321 ggaacctccc tcactgcgtg ggacattcct ggcctccttg atctgcttta caaccacctt
     7381 aagcgaggtt atcttatctg tcagtccgtg ggttttacga ccgtgatacg tcgtttttcg
     7441 gagaaaggag tgagtgcaat gaagaagcta aatgttttgg tcatctgggg attgatcctt
     7501 ggtttgtgtg tcctcagcac ttcgagagga atggtcgcag ccgaagaggc agcggcaaaa
     7561 gaggaggaac cgaagtttat ccgcacacca gatgtgatct atgttccgac gccgtacgat
     7621 gtcgtccgca agatgttgga attggcggag gtgaaaaagg gcgacgtagt ttatgacctg
     7681 ggctgcggtg atgggaggat tgttgtcacc gcggcaaagg aatacggtgt caaagcggtt
     7741 ggttttgata ttgatcccga gcgggtcaaa gaggccaggg aaaatgtgaa aaagaacaag
     7801 gtcgaacacc tcgtccggat cgagcaaaaa gatattttca cactggatct gagcgaggcc
     7861 acggttgtga ccttgtacct tctgccgcaa ctcaacgtta agttgatccc tcaattggaa
     7921 aagctcaagc cgggttctcg gattgtgtca cacgacttcg atatggccgg ggtcattccc
     7981 gacaaggtcg tgaatttcaa atccaagaat ggtcgtgagc atacgctcta cctctggaca
     8041 acaccgctga aaaaagagaa gacccaataa cctcagcggc gtttcggccc aattacattc
     8101 gcagtggctc atttacgcta ctggtttggc ttatgttcga gggttgatct gataagctgc
     8161 cagtagtggg aaacgaagac aagccgccgg ttgccgcctg gttcgtccct gtccccggga
     8221 cagtcgtatt gctcgactga ctaccggttc gcgggatgta agtcttcgtg cttccgggcc
     8281 ggtattcgcc agcggagttg gtactggtag gccaacttct atccgatgcg tttgtctgct
     8341 ggctattcgt gccgtaaggc atagcgtatg gcggaactcc cggaggattg tagccggttt
     8401 gacccggccg gtaattgaca tttccgggcg ggttttctag cttgcccggg acgtatcccg
     8461 tgtcgccggg cgaatagccc gtacttccaa ggccgtcggt ccctgacggc gatggcatgt
     8521 tactcccttt ttggtagagt tgagcgtacc gctccccaac cagcgtcgtc cagtccgcgg
     8581 tccccgcgtt cgtggcttcc tggaaagccg attgtgacgt ctttgattcc ggagtcgctg
     8641 cagacggacg atagttagtc cccgatccat ttacttggga tgatgattcg gcagagggga
     8701 gagtcgcact ctgtgtcgtt cgggagtaac tgggaacata cggatttgcg ggcattgaac
     8761 ccgtgttgcc tgaggacgca ggctgagaaa agttcctttg atcactggcc gtggcgactc
     8821 ctgccgggtt ggactgccag gacgtggcgg taacaggatt tgccgaataa ccaccggagg
     8881 gcggtgtggt ggtggttgca ggatatgatt gtgctgtggt gccataaggt gttccagaac
     8941 gcatggatgc tgtacccgga taagtggtgg attcaacatt ttggtaccca gcctgcgtac
     9001 cgggatacac ttgggcgctt gcggctgccg gcagtgttcc acctttggtc gagtcggcaa
     9061 cggtgccgga atatcgccct gtggcatcgc tcggcgaggc gaaagaagac ggtttttgcg
     9121 ggtatggctt ggtttccgat tcgggcttga tccagtggaa ggggttaact ctcgcgagat
     9181 tgaaattgga accggtcttg ttcgttgagc aacctagcag gacaaggcca gtcaccaaca
     9241 cagaaaaccg tcggattgag gcgagcttca tttctgggcc atccttggct aaacagttcc
     9301 ctgggatatt agcgggcacg aatggtcttc gccggaatgc gagtgaaaaa ccctatccaa
     9361 ccaatactgc cctcgtgctt gatcactgcc cccaggctcc ctggaggatg ccgaaagttt
     9421 cgcacagact cggcatgacg tgctctcaaa aaacattccg ggaaggcgga cacgggcgat
     9481 tttttaccgc aaaacttgtt ttcgtgcaag cccgttttca atcccccacg aagaggggca
     9541 tcaacgggat aggaactaac cgaagccccg cattgtaacc tgttttgctt ttgtctggtt
     9601 tagttcgaga acgcgttgtg tcccttttca acgacccggc gccgctgatt tgataatact
     9661 ccagctcgag caggtctagt gtttacccac ttttgacact gtaacatgcc gggaaacatg
     9721 tggtgatgcg ggaatgcttc ggtcgcgcgg cactgaaaaa gaatcgggaa tttgtgagca
     9781 tttcggacga tcgacgacgt gaaaatggga cgtaagagac gcaattcaca gcaaaacgac
     9841 aaaataggca agatgcgcat tttgccgcac aagccgtact ttttgtcgcc accgtgcccg
     9901 caagtttcac ccgaaacgta ccgtggctgt aagaaaggca aggaaacccc gggactccgt
     9961 accttaactt tctgcgacat ctcacctaaa attggttttt tccttgtaat ccgttggttt
    10021 tcgcaaggag ttacgatcca gatgattgtc ggtgtaccgc gcgaaattta tccggaagaa
    10081 cgtcgagtcg cgatcgtgcc ggctactgtc ccgtcattaa cgaaggcggg ccatgttgtg
    10141 cgcgtggaaa cgggagctgg gatggccgcc gggttctcgg acgaggagta ccgctccaag
    10201 ggcgcagagg tcgtttcgcg gcgtgatgtt tttgcttctg aggtactttt aggagtccgg
    10261 tgggatatcc caggcgaggc gcgagcggaa acactcgatt tggtgcgtcc aaaacaggtg
    10321 atcgtgggga gttgggaccc tctttccaaa cgagagaact tggtagcctg ggcagagaag
    10381 ggggtttcca tcttcgccct ggagctactc ccccgcatca cccgggcaca aagcatggat
    10441 attctttcat ccatggcgac cctggcagga tatcgtgcgg tcatcttggc tgcgatggca
    10501 cttcccaaaa tattcccgat gctgatgacg gccgccggga cgttgacccc agcaaaagta
    10561 tttgttgtgg gtgccggcgt ggccggtctc caggctattg ccacagcaag gcggctgggg
    10621 gcagccgttt ccgcttatga cgtccgcccg gcagttaagg aacaagtgca gagcttgggc
    10681 gccaagttta tcgagttaga tttggaaacg agcggggccg aggaccagcg cggttacgcg
    10741 aaagccatgg acgagtcctt ctatcaaaaa caaagagaga tgatgtttcg agtggtagcg
    10801 gaaagtgatg tcgtaattac aacggcatct gtgcccggta aaaaggcacc cgtcctgatc
    10861 acgcgggaga tggtggccgg catggccagg ggttccgtca tcgttgattt ggcagcggag
    10921 cggggcggca actgtgaatt gacgcagccg gggaaaactg tgatcgaaaa tggagtcacg
    10981 attcttgggc caacgaattt gccgtccgag attcccttcc atgccagcca gatgttttcc
    11041 cgcaatatct caacattctt cctgcacttg tgcaaaaccg gcagcatccc atttgagacg
    11101 gatgatgaga tcattcgcga cacacttgtg gtacgggatg gagagattgt tcacccgatg
    11161 ttagtgggca gctccgccgg ggcaaccgcg tgattggtca tctcgaaaga cgacacggga
    11221 gagttcgctt atgcttaaca attgtttttt gctcgctgaa cagatcaatg aagtggccga
    11281 ggtcactcaa ggctcacaac tgcccttggc cttggcattg acgattttcg tcttggcaat
    11341 ctttgtgggc tttgaaatca ttaccaaagt cccccccacg cttcacacgc ccttaatgtc
    11401 cggctccaat gcgatctccg ggatcacgat tgtcggggct atcatcgcgg ctggggccgg
    11461 acactctggt ctggcaaccc ttctggggac gattgccgtc gtccttgcta cgatcaacgt
    11521 ggtcgggggt tttctggtga cgaaccgaat gctccatatg tttaagcgaa agtcctagcc
    11581 gggcagcaag ttggtcgttc ttaacatcgg cgcgattttg caaagacgtg gtcatttcgt
    11641 aaaggtgagg tagctgtgga tcgcgggact gtcataaccc tggcatatct tctggcttct
    11701 gtcctcttca ttttggggat taaaggtctt acacatccga gaacggcagt gcggggcaat
    11761 ttgctgggcg ccctgggcat gctaattgcg attgtggcca cgcttatcca ttggtctatc
    11821 atcgaatacc attggatcat tcttggattg gttgtggggg cggtgatagg tgccgttctg
    11881 gcgttaaaag taaagatgac cgccatgcca caaatggtgg cactccttaa tgggtttggg
    11941 ggtggggcgt cggtgctggt ggcaggggca gaattaaccc gtacaccgcc ggcaccttat
    12001 agcgatgccc tgattgcaac ctacgcttct gccgtcatcg gcgccgtaac tttttgggga
    12061 agccttttgg cgttcgccaa actcgaggaa tatagggcct ttcgccagcc gattcgttat
    12121 ccccttttgc agccaatcaa tgcggtccta ggacttttgg cgttggcttt gtgtgtgatt
    12181 ggcatcggcc aaccccagta catcggtctt tattggggtg ttgttgccat tgcatcggtg
    12241 ctgggtctga ctctagtaaa tcccatcggc ggtgcggata tgccggttgt catcgcacta
    12301 ttgaactcgt acagcggatt ggctgccgct gctacgggtt tcgttctcgg taatgctgtc
    12361 ctcatcattt ccggttcttc agtaggggca tcgggcatca tccttacaaa cattatgtgc
    12421 aaggccatga atcgttcgat gacgaatgtc ctgtttggtg tgctccaagc cgacgagggg
    12481 gcagccaagc aggacgatgt ctatcgcaac gttaagggcg cggcggcaga agagatcgca
    12541 atgctttttg acacggcccg acgtgtggtc attgtgccgg gttacggcat ggcagttgca
    12601 caggcacaac acgccgtacg ggatctttat caattgttgg aatctcgcgg aattgaagtg
    12661 ctttttggta ttcacccggt tgccggacgt atgcccggcc acatgaatgt gttactagcg
    12721 gaagccgaga ttccctacga caagcttaag gaactcgacg agatcaattc cattctctcg
    12781 gaaaccgatg tggcgattgt tctcggggcg aacgatgtgg taaatccgct ggccaagacg
    12841 gatcccaaaa gccccatcgc gggaatgcca atcattgagg tagataaggc taagacggtg
    12901 gtcgtgatca agcggagcct cagcccggga tttgccaata tccccaatcc acttttcgcc
    12961 gctgataata cgctcatgtt ttttgcggat ggcaagcggg ccgtccttga cctgattgca
    13021 gcgatcaaag aaagttgata aaacctgacg gcagacgtcc tcgacagtcc tgttcgcaag
    13081 acgtgtcgat tttgaatcag cttcgagttg ggagcagcag taggggaggc catgccatgc
    13141 ggcaaccatt gcagaattgc ctgttttgcg catttttgct tgtcttcatg ggaaatgcgc
    13201 cgttgatggc agagagccac acggttcttg gggttgaggg ctcacgtttt accgtcaatg
    13261 gccagccaac ttttcttttg ggcgtaagtt actatggcgc tttgggggtc ggccctgagg
    13321 tatggaaatc cgacctgaaa gaaataaaga aacgcggctt caactgggtc cgcatttggg
    13381 ccaactggaa tgccttcggt caagaagcat ttgcggttga tgcggaggga aacccttatg
    13441 atgcgggtct caaacggctc caggctattg tcgcatattg cgacgagctt ggccttatcg
    13501 tagatgtaac gttgtcacga ggcaacgggg tgacgggccc caagcgactt cagactctcg
    13561 atgcgcatct gcgggcggta cgcgtactgg tgaccgcgct gaaagactac cgaaactggt
    13621 acctggatct ctccaacgaa cgaaacatcc gcgatgcacg cttcacgtcg atcgaggact
    13681 tggtcatttt gcgaaaggaa gttcgtacgc tcgatccaaa acggttggtg acggcctctc
    13741 atgctgggga tatttcggag gaagagttgc gagactacgt gctgaaagtg ggagtagact
    13801 tcattagccc acatcgtccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    13921 gatgggcgac cacgaaggtc attcgatctg agagacggca acctatttac gcaattggat
    13981 tctgaagaaa agcgggcgat tgagttgatt gcgcagtttg cgaaggatgt ccttataaaa
    14041 tgagcggtat tctctcctga ccaagaacct cattcatttg tgtatccttt ttgtccgtca
    14101 atcttctttt gtcgtttact tattttttcc aatttggagt gactctcgag gggcggcatt
    14161 tcttatccca tcgcgagctt tgcgagaacg ggttcccttt gtacccagct gacgaggtcc
    14221 gaaacggcac gaccgatttc tttctcatct cacgaaaaac ctgcaagttc agagaagttt
    14281 aacacgataa aagaggtagc cgctgtttaa cttgcggtct ccgttagcag gccaaagtaa
    14341 agcatttccc ctctgcgttc ggtgagttgg cgaccgggta aacagggtaa atcgctcaag
    14401 cattcagaac ggtcgaggag ccattcatgt tgcggaaact tggatatcta ccagttctcg
    14461 gtgtcctggg aaccattgcg gttttcaccg tgggatgtgg gaccgtcatg agtcctcttg
    14521 actggggggt tctgaattac ccgattcctg tctcacctta cttccaggac aaactagagg
    14581 acaaggcttg ggaggaagaa cggtataaaa agatgccgat cctgcctccc ctcgttgagg
    14641 gggcgccgca cgtggccctg gacgagcctt ctgatgacga agttatgcgg gcactggaga
    14701 aggcccgccc ccttgagggt ggcttgccat tcttgcatga agtccagcgg agcaacgtgc
    14761 ggattgtcaa ggagttgatt gccgactcga tcgatccgcc tcgagtttat ccactcgtcg
    14821 gtccggccca gcttcatcac gtccattgga agtgcaccgt ctatttcact gagaccacca
    14881 aagtgggttg gcctattcct tacaccgtaa aaaacgaaga cgcagttgag gtgatctaca
    14941 ttgaccacga tcacctccac atggtgggaa atgtggacgg ggggccgagc agcaactatt
    15001 gaggcaacgt ctagacaagc cgcacaattc aatcaaaaca acagaaagcc tttcggaaac
    15061 ggacctccga aaggcttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 gggatttggc ccaaacccaa atactggcca agtggccacc caaaaacacg cggacaggtt
    15241 atccgctctt tcaagatggc gggaactatc ctcaaatcca tccgatattt caagaccaat
    15301 ttttatgcga acgatgctga cgagctcgcc gtatccgaat cctctacgta gtgattgaca
    15361 gaccatacct gtccggcgca aactggtagg cggtatgggg gtgactgaac gaggttctga
    15421 tctcgcggaa aggagatagt catggccgat tccactcgac gtcagttttt gcgatcggca
    15481 gggaccgccg gaatcggttt gacggtttct gtgtccaagt caacgagaag ggccatcggc
    15541 gaacaatcgg cccctgtagt ggttccgtct cccaacgaga aacccgcctt gctaggaggg
    15601 aaacccacgg taaccggcca atggccccat tggccgtggg tttccagttc agatgaagaa
    15661 gctgtgctag aggttgttcg ggcagggcgg tggtatcgta gtcgatcggt tgagcaattc
    15721 gaggaggaat acgcagcgct caattcggcc cggtattgta ttgcaacagc gagcggcacc
    15781 acggccctgg tgacttctct cggagctctc ggcgttggac caggagatga agtgattgtg
    15841 ccgccgttta cgttcctggc gaccgtcaca tcggtcctcc tgcattatgc gatgcccgtt
    15901 ttcgtggatt cggatatcaa cacctttttg atggatgcgg agaagttgga atcggcaatt
    15961 acggaaagga caaaggcgat tattccggtt cacattggcg gaaatgtggc caatctggat
    16021 cgtattctgg cgattgccga gaaacaccgt ctggccgtcg tcggcgacgc ctgtcaagcc
    16081 catcttgccg agtggaaggg caagagtgct ggtagttggg gaaccaccgg atgttatagt
    16141 tttcagctca gtaagaatct ctcgtccggc gagggtggcg ccattttgac taatgatgaa
    16201 gccatggccg acgcttgctt tgcctttcac aactgttacc gcaagcgtac aagggccagc
    16261 ttggaagcta aatatcactt tgggcgaaac gccaatttcc gcatgacgga atttcaagcg
    16321 gccttgttgc tcaatcaaat gaaaggcatc aaggaacggg cacaattaag gagcgacaac
    16381 gctgcttatc tgacgactct attggcgtcg attccgggga ttcacccggc caaggaatac
    16441 tcggggtgca cgcgaaatgc gtatcatttg tacatgtttc ggtatgatcc caatgagttt
    16501 aacggcctgc cgcggaaccg tttcgtgaat gccctgcggg cggagggcgt gccgtgttca
    16561 ccgggatatg cccctttaaa caaagagcag ttcctgatgg acgccctgca ttccgaaccc
    16621 tacgtgcgag tttacggccg tcaagcgatt gagcagtggc cggagcgcaa tcagtgcccg
    16681 gaaaacgaca aattgtgcga gcaggccgtt tggttctatc agacggtcct tctgacggat
    16741 cgcacgcgga tggagcagat tgccgcggcc attcaaaaaa tcaagaaatt cgccggcgat
    16801 ttgcagaagt cctgaagtct agacgttgct ctaggttatc cattttcggc atttcaaggc
    16861 tgtacggacc acggttaagg cgcttcagtc agcgcccaac ggctattttc cgctcaaggt
    16921 ggctactgcc tggacaaatt tcaggtttac gggcctcgaa tcgccgtaca cctctacgcg
    16981 gttggctact attctcgccg gaccgtaaag aagcggcgga attccttttt atgtccatcc
    17041 ttttcaagaa taataaccac cagtccttct tccccacgag ggttagctcg gcatccgaat
    17101 cgaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgat ccagtgcaga
    17221 atccgcacgc aggttgtggg gtaatccaga aaacgatttg gatacacatt gccataaggg
    17281 tggcagcaga tgtaatacca aaactcgact tggcccgacc gcttgagaaa tacactacgc
    17341 caggtgtccc attgtgacaa ctttgggatc cagatttcca gggcaccgtc gaagtcgatg
    17401 gtttcaatgg catcaattcg tcggagacgc ggtgccgcac gatgaactag ctccgaggct
    17461 gctcgccacg aagcgacatt ttgaatcgct ggttcatcgg aaatgtggat ggcggccttt
    17521 tcaagccaac cttttgcttc aaggtgccgt tcgaggtcgg caagcaggag tttcaatcct
    17581 cgctcgtagt ccaaggatac ggtttccccg gtcgatttgt cctttgcccg gaggttcgac
    17641 aattctacag gtccacccca acctcccttg gggtgcccca catgggagat ctcgatgaga
    17701 tctgccaccc ccattttctg aaatagttcc acgaaccgat caaagcgaga atagtcaaat
    17761 gtcaattgtc catcaggatt ccttgttacc tcaatcaagg tccaaggtgt aagaaccaca
    17821 ttttggcggt gttggcccat gttctgggcg tacttcgcta ggatatccca ataggcatcg
    17881 gaccatattt cgacctgatg ggccttggcg atatttcccg ggctgaacca attggtcacg
    17941 aacagatgac gctcgtcagg aagagcgaaa ggaaaaacgt gcaattcgac tgggatcttt
    18001 gcttggttgc cgtccgcaac aatcgccaac tccccgtgat agacccctgg gacagttccc
    18061 ggagggatcc aaaacgtcac ccaaattggt tggcttgtct cggctgctag gctgatctcg
    18121 gtctcgtcca aaagcgggtc ggggatttcg caaggcgcgg ctgccacacg gattcgctcg
    18181 gcatttggcg tgtttgcctt gagtggaata tagccgacga accgcatgcg aacactctcg
    18241 gccggcagga tcgctccgtt ttctggattg cgaaattcag acgcccgaat tgtcagccgt
    18301 ttgatttctc tcgcggttcg cacagcgact tgggcagatt catattcgtt ttgggctgcc
    18361 ttcacaacta acggaagttg aatatcctct ggcggctggg catcacgaag cactttgaca
    18421 tggacatcaa ccggccaaat gacaacgggg agtttgtcct ccgatcgccc ggcaacactc
    18481 cacaaacacc aggcgagaac gccgaggcaa aggggtcgta tcttcatggc ttgtccctcc
    18541 tacgcgcagg ttactttgaa aatgtgttcc gataggatct tgggtactac atcactttga
    18601 cgacagccca ttgtagcaat gtaatcggtg gtggacatcg gactttcccg atttggaccg
    18661 gcccactcct ccgttcacgc gagcttccgg tcggtaccaa gcttgtgcgc gctgtctgtg
    18721 gtgaaagcgg gaccctcagt ttcatttcgc cttgacgggc ccgtcaaaaa ttggcaaact
    18781 gcctgttgtt aggcgttggc ccgcaaggca cccgttatcc gcggggtcac gaaggaaatg
    18841 gtctgtcccg caaagagtgc aatttctatg gggttaatgt cagattcgcc ctacagctcg
    18901 gtgcgataag cagagggctg tattctgcct tcctaaggca tgcaagcacg ttatctagca
    18961 agtgacaagg aggtttttat gacaaaacac ggaagtagcc ggggcatccg atcacaggtt
    19021 tattggctta tttgggcatt ctgtgtgctc attcctgtgt tggcaggatg taatagtggc
    19081 ccatcccagg agcaaccaaa aggtggggaa cagccggccg ctagtaattc ttcccaaaac
    19141 ctgccgcagg tttctcaatc tgaattgcag aaagtcactg gtgatcctgt atccgcagta
    19201 aaggtttttc ttgaggcggt tcgcacggga gatgacgaaa gggtggtagc gctcttttca
    19261 gaagaggctc gcaagcaggc gggccagctc aatcgccaat ttgcgcctgt cggtagcgat
    19321 accgcgcggt atgaggtttt tgaggacgtt caatacctgg cgccggatgg agcccgcgtg
    19381 cggacgcact ggaccgatct cgattcacgc ggtcagcccc gcactgatga aatcacctgg
    19441 atgcttcgga aagaggcgaa cggatggcgc gtggcgggaa tggctacggt gatattcgaa
    19501 ggcgagccgc cacttctcct cgattttgag aatatgcaag agaccctccg taaggtcgaa
    19561 atgttgtcgg aggaaattga acgccggcaa gaagcggctc aaaaggtggc ggccaagcaa
    19621 gaatgggaag acgccggagc agaaaaccag gcgtcaggcc aactgcccaa cagcccacag
    19681 caagacccaa gcgcagcaga cttgcccgcc gctccgggcg tcaacgacgg ctcgaccatg
    19741 acggccctta agccggacga acccatcaca agagagtaac gaatcgggtg actccgcccc
    19801 aagccggcgc cgccaggacc tcgtgctacg ccgtcttggc cgttgggaat aacgaccgca
    19861 agaacttctg attgtcacgg taacagtcca ccacagatcg ccctttaacc gtggcggcct
    19921 caataatcaa ccagtcgtaa tagccgattt cgccgaggac atcacgaatc tttaggaagt
    19981 cgattggtcc ttggccgagc aaatggttgt attccttcat gtgaatttgg cagattcgtt
    20041 cgcgtcccag acggcgtagc tcggcgaaga tgtcatatcc ggcgtaatgc atatttgcca
    20101 catcgtaata gacttggacc gccggtgaac ccacaccgtc tagaatcctg aggtggtcat
    20161 ccacattgag cgtgctttcg actcctaaga ttacctgctt ttcctcggcc tttggagcaa
    20221 gttgtttaag tcgccgaatg acctcatctt gtaaatctcg tttacctttg atatcgccct
    20281 tcccaaagaa ggccaaaagg acaatcttct ggcccatggc ggccatcaca tcgatgcaat
    20341 cgctcaccca ctctacggtt cttggatcgc tggaaaacgg aatgttgttg agcacaccca
    20401 tggccaagga tgcgatctcc acccctgtct tggccgcggc ttccgcgtac tgttggcgaa
    20461 ccttttggtc acggaggtca tagctcccgc cgggttcgcc gaaactcact tgtacaccgt
    20521 ccagaccaat ttccttggca acctcgaagg cacgaatatc ctgtctcgct ccgatggacc
    20581 aatcgcaggc accaattttg tataggcggt tctggtctgc gagcaatggc cgaagtgaag
    20641 aactggccaa caaacctccc actgacgtga gcatctcgcg acgtgtcaac ggcatcgttg
    20701 agtctccctg caagcagctc aaaagggaca acttcttgca aacacgaaat ctagtttaat
    20761 ccaagggcgc gggcattact atccaacgag aagacacccg aaggttcgtg ggaaaaaaca
    20821 aaaaattaag agcctatccc ataatctctc gtcacccgta cggcgggaga ggggtcgggg
    20881 gtgagggcgg ctcgaatcgc tgaaggtttt ctcgatcgcc ccaaacgatc gattgccctc
    20941 accccaaccc tctcccagag ggagaggggg acatttgcga tagactttaa ggtaggggtt
    21001 actggttggc aagccagggt ttggagcgaa cgcgttcagg ttcactcaaa gggtgctcaa
    21061 agccgggagg ccgtccaccg tcgatcgcag gggcagtgcg gtcctcggga tacgggtcga
    21121 aacctttcca ataagacgtc atcgacgagt cacgggtttg atacacagct cccccgccgg
    21181 tacagccggt ttgggcgagc acagagagaa ttaagcaaat taccaggaaa gttctcatgg
    21241 ttttctctca caccttatgg gtaggtgtga aaatcatccc ataaaacctg aaaagaaaca
    21301 atcgaagttt ggtgcttttc tcactggagg gactatgacc acttgacgga tttcgcttag
    21361 attcaataat atcctgtaag gaagatcaac gattcagcat gaaggatcgg cttccgcgga
    21421 cctttgaatg tactaggagt gatttatgaa aggtgacgaa aagattctca agaccttgaa
    21481 cgagcttctc aaggacgagc tgacggccat cgtgcaatat gtagtccatg ccgagatgtg
    21541 tgcggactgg ggctatgaac ggctacacga ggccattgag aagcgatccc ggcaggagat
    21601 gaaacacgcc gagagtctga tcgagcggat cattttcctt gagggcaccc cgattgtatc
    21661 tcaggtcggt gacgtccatg tggggccaga tgtgcaaacg cagcttcaat ccgatctcaa
    21721 tgctgagatg ggggccatcc aggcgtacaa caaggccatc caattggcta tggacttgaa
    21781 cgactcggta actcgggatt tgctcgaatc gattgctaag gacgaggacg atcatatcaa
    21841 ctggatcgag gaacagctgg cccagatcga acagatgggc ctgcccatgt acttgnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcga tcgtagcatg tcccaagaca
    22021 aattgagacg tcgcatcgca tgggaagcgg cacggcttat atattttcgt gaggaaacgg
    22081 aataccaccg cgccaggacg aaggcggcca gacggatttg tcgcggcgag gcgcctctgg
    22141 gggacttacc gacaaatcgt gagatccggg aacagatcga ggctgtggcg cgagcctttg
    22201 agggggaacg ccggggtgtt gaggtcggcg aaatgcggct cgaggcactc cgaatcatgc
    22261 agattctgaa agagtaccag ccttatcttg tcggcggtac gctcaccggt catgctcgtc
    22321 gaggctcgcc gatcgacatc cacattttct ccgacagcgt tgaggcggtt caggacacgc
    22381 tacaaaagtc gcacatcccg tttgagattg tcaacagacc aggtgaaaca gcaggctccg
    22441 agaaaagttg tgtttgtttt cacgtcacat cccggtttgt ctgtcggcta actctgtatc
    22501 cggcggcgat ggtcggtcaa ctaccgatca atgtgacgac cgggcagtca atcccacgag
    22561 cgaatatcgg agagttagag cgtcttattg ctcgagagca tcccactctc gtttatcgta
    22621 cgtgtcctca ggaggaaccg gccctggtcg atcgtttttt ggcttatgag tcactcctgg
    22681 tgccccttga gcacattcga gacgaccgta agggtcagcc agacatcgac ctcctaacac
    22741 acagtcttca ggtgtttcac ttagctcgat cggaatcgcc ctacgatgag gagtttcttt
    22801 tggcggctct tcttcacgat gtgggaaagg cgattgaccc aaaagatcac gtcgcggccg
    22861 ctttggaggc tctagacgga tacataacag agcgcacggc ctggctgatt gcccaccaca
    22921 tggaggggct gcaagttttg gatggcagta ttggccagcg tgcccgtcgg cgactggaaa
    22981 gctcagagga ttatgattta ctcgtcttat tggccaggtg tgatcgtgcg gggcgacagg
    23041 taggagtcgc tgtcccagac ctggaagacg cgattgagta tcttcgtgcg cttgatcgcg
    23101 actttggatg attgaccggc tgtcacttct ctttgaaaca tcatcttact agggcctcca
    23161 cgccaccggt ttcttcgatt ctgggcactc tctatcgttg actgccttat taccgtagaa
    23221 tgaacaccac atttgccgag tgcctcgcaa tagtgtcccc cagagaggct ccaaccaggg
    23281 agttattgtc gatgaatcgc ctcaagaacg tcactacctt gataggtctt gtgcttctgg
    23341 cggctagtag cttgagcacc gtgcggctac gatccacttt tgcggtggaa gcgatgcacg
    23401 acacccaaaa ggagaatgca ccgggggtca gttggcacca tccgctttat ctccattggg
    23461 gcgggtattg gaagagccgc gttcgggtca cctatcacaa tcgtggggcc agggatttgg
    23521 caggatatcc cgttgctcta cccgtcgtaa gccctggtag tttttcggcc ggcgcagctt
    23581 ctgggattcc tcttgcggga cgcaatattc gcgatattcg cgtctgcacg gctgacggaa
    23641 ccgaattact ttgggcagcc tgcgacgaag atatggtgtc tttcaccgag ggaacatttc
    23701 cgcccaaagg atggctcgtt gtgccagtga catgtccggc aagaggaaag acggaattct
    23761 tcgtctattt tgacaacccc gaggccatcg aggtacctga ctacttctcg gccaagccgt
    23821 gcccgataaa ttgtggcgta gaaaagggcg aaggggaaac accctcggga tggcagcacg
    23881 atgccagcgg tccggaccga cgggcctact ggagcaacga gaatccgcac tccggggcaa
    23941 aatgtctggc aattgaagtc cagccgggat caaaaccaag ctggattgct actcgacaga
    24001 ccggtgttgc cgtggtgcca ggggttcgat accgctttcg ggcatgggta cgggcagaaa
    24061 acgttgaggg atacgccggt tggtacatcc atgtgggaaa tgcagaaaac cccatgatga
    24121 ttgccccgac catctccggc ggtggtggaa cttacgattg gaaacaagta gaagccgaat
    24181 tcacagcgcc cgagggggca accaccgcca gtctcgggac ggccctttgg ggcacaggcc
    24241 gcgcttggtt cgacgacgtc gagtgggaaa tggacccaca ggcctgcgag gtcgtcatcc
    24301 attcgccaga atcgtcagcg ctggccgagg tggacgagac gcaagcttgg ccgtcgtccc
    24361 aagaaaatca ctttttggga aatcagagac tacatttgga gcttctcaat tttgacacgc
    24421 aagccaggca aaacgtgctc gcgtgccttg ggcccaacgt ttggggtggc cgattaccgc
    24481 tgttagccga aacagatagg gttcgcatgt ttgcggggac cacacgcatt aagcctttcg
    24541 ttgttgggga atacctcctg tggcctgatc atttaccggc aagatctcga gtccatcgct
    24601 atgtttactg tgaggaccct acatcagtgc aacccatatc agtgcagccc atcgaggaaa
    24661 aggacgctac taacaaagtg ctccttgaat atatggccct gcttaatagc agtaacaacc
    24721 tcgtacagaa cttttccttt gaagctggcg gcgatgtccc agcgggctgg ctccaatccg
    24781 gggtagggga cggtcgcgtg aagttttctg tggaagatcc gaaacatgag ggttttggac
    24841 ggcgtgcggc cagactggac ataccttcgg gaactccctc agcttggcga ggctggcagc
    24901 aaagaatccc tgtaaaggcc gggacaactt accttgtggc cggttggctt aagtgtcaag
    24961 acttggaagg cgaggcgcgt attcacattc acttccatga tagccagggg cgattgtgca
    25021 aatcaggtgg aatgacaagt gttgggccgg gagttcgtgc cacacagcca tggacccttg
    25081 tcagcggtct ggtcaagact cccaacgatg cagcaagcct tacgcttcat ctcaccacca
    25141 acgtgtcggg aaccctttgg cacgatgcca tcttcgtggc cgaggtgtgc aatgtatcgt
    25201 gtgttaagcg agaagccgca cctatggcaa aggacgcttt tgtcgtgtgg caggtccctg
    25261 ctgtcgtcaa ggtttttcga gaggacccac ccgtccgtga aaggcagtcg atccaggtgt
    25321 ctgctgcaaa gggagagtgg gaagcattac aactggccgt gcggagtggc acgtcactgg
    25381 ccgaggtgcg ggttgaagta ctgcctccta gaaatcggaa taacaaacag ttggaccgat
    25441 ttgaggtgac cgtggttggt tatgtcccca tcgattatcc aacgaactat taccggtttg
    25501 atggacccga atggcaccgc aagattccac aacaatcgcc cggcagtgat gggtggcctg
    25561 gctggtggcc agatccgctt ttgcccaaat cgcagttctc cttggccgca gagacaactc
    25621 aggccgtgtg gatcgtcttc aagatcccac aagatgccga atctggagat tatgacggaa
    25681 ctgtccgttt catcgggcaa gggcgtgttt tggccgaggt tccattttct gtccacattt
    25741 ggaattttgc actgccgcga gagacgacat tcccggcaat ttacgatgtt cgattgggac
    25801 cggccggacg agtttttgga caaacgttgg accagttgta tccagagatt gtgcgtttca
    25861 tggcggaacg ccgtttgtgt ccggataaca tccgacctga ccctgtcatt gagtatcgtg
    25921 atggccaagt gcgtgccgat ttcaccgaat acgacagagt ggcaaggtgg tattttgata
    25981 ctcttaagct accggtaacg tacacacctc acgtgtttta cctgttcggc tggggattcc
    26041 caccgcggaa cttttttggc caaacgccgt atcctggtga ttctcccttc gaaggtgtca
    26101 atcgcagcga gctccggccc gaatacgtgg cggcgtatca agcgtgtctc aaatgttatt
    26161 gggaccacat gaaagagaat ggctggtcgg atcgtgtcgt tctttatatt tccgatgaac
    26221 cttttgacca ccttcccgag attcgagaac aaatgattgc cctttgcaag atgattcatg
    26281 acgccgttcc ggagattccg atctactcga gcacttggca ccatatcccc gattgggatg
    26341 gtagcatcac cgtttgggga ttgggacacg atggccgcgt ccctccggag aaactccgcg
    26401 ccctccggga aggaagtgcc caggtatggt ttactactga cggtcaaatg tgcaccgata
    26461 caccgtactg tgctgtggaa cgattgcttc cacattactg tttccaccac ggtgttaggg
    26521 catacgagtt ttggggggtg gcatggacaa cctatgaccc ctaccggttc ggatggcatt
    26581 ccttcattcc ccaaagtgac cgccccggca ctagatactg ggttcggtac ccaaatggcg
    26641 atgggttcct gatttatccg ggcaaaccaa ttggatacga cggcctcgtc agctccattc
    26701 ggctagagca agccagggag ggcgtggaag actacgagta cctgagcatc ctcaagaccg
    26761 tcgcaaccaa ggcacgcccc gatgatccga gacgtaaagc ggccgagagt attttggcaa
    26821 aagcctccca gttagcgacc atacccaacg caggtgggcg ttattcgaca aagattcttc
    26881 ccaaccctgg gatgctcgag gaaatccgca aagagcttgc cgaggccatt gaacagtttg
    26941 tttcaaatca gtaggttccg gctgccagat aaaccaataa gagaccagtc acttggtttg
    27001 gcagtcgtgc aatgaaagtt ttcgtgacat agcagaatct tgcaggacgg atgatccgtt
    27061 tttgggtttt ccatggtccg gagcggttat gcagacctta ttggaacgtc tcgtgttttc
    27121 ctttgggtgg gcaaagtctc gcattttgtg ccgtcgtgtc tgtgaaggtg caagcgttgt
    27181 gctggtcatt gctatgatga tccacggtgc cgtgtgcact ttggctttgg attgtcaagc
    27241 ggagaagcca cgactcaagc cccggattga aggcgactgg tggcaagttg ccgaaaatcc
    27301 cgacttggga ccactcacca gtgaacggca gcaagtggtg gattttggaa tctggcaagc
    27361 tgaggacggc acgtggcaac tttggtcttg tattcggcac acaaaggagg tgggacacac
    27421 gcgactcttc ttccgttggg aaggccgtca attgactcag tccccttggg aacccaaagg
    27481 catcgccatg cgagcggatc ccgattacgg tgagcaaccg ggcggacttc aggcgccgtt
    27541 tgtctttcgc atcgacaagc tgtttcacat gttttatggg ggttgggacg tcatctgctt
    27601 ggccacgagc caaaatggga aggattttgt ccgcaaacga aacgaggcgg gtaagagttt
    27661 cctatttggt ccacccgatg ggtacggtaa ccctcgggac ccaatggtgt tgcgtgttgc
    27721 tgaccagtgg tactgttact ttacggccca tactcctcac gagggtgccg tcttttgccg
    27781 gacatccaag gacctagtca actggagtga ggttttcgtg gttgccaagg gtgggcaggc
    27841 aggctcagga agttattcgg cagaatgccc atttgtggtg gaagctagcc cgggcgagta
    27901 ctaccttttc cgaacgcaac ggtatggggc aaaggcacaa acaagtgtct attggtccaa
    27961 caatccgttg gattttggaa tcgacaacga cgaggggcac tttctctgta ccttgcccgt
    28021 tgctgcaccg gagattttcc gccacgcaga cagctggtat attgcggccc tgatgccgaa
    28081 cctttcggga ctccagatcg cgcgactcaa gtggacgacc gacgattcca tgccctaacc
    28141 accagtcgta atcatgcgtt cccatagcca aggcgccaac gtacttctca tacctgccaa
    28201 accttgcggg ttttagccat aaccctagca tgcacctccc gagggggcct tcctacccag
    28261 attctcagtt ggtcctctat tccttgcctt ttttccgtaa ccattaaagt tttctttgct
    28321 tcacgccgac aataaaggca aactttggcg gcagcgaggt ccctcgggtg gcagttcgct
    28381 tactagacag atcaggttat gcatcaatac agcattgacg ccgtcttgca atgtctctct
    28441 gaaggtcgcg cggagataga ggcgacattc aagcgagcgc tcgaccagtt tggctctctc
    28501 cagctcgacc agatcagcca tagtagttgg gccgacgtct cagggagcga ttggatcggg
    28561 cagcccggcc tgctcatcac gtttcagatt aagcaggcgg acgttgtttt ggccttgccc
    28621 caatcgagcg gatgtattcc tgattgggcc taccaaccgg atgccacggg gaagtctcgt
    28681 ctcgccacgc tggcccaaga actgagcatc ctcatttttc ccccagagtg gccagtgctc
    28741 acttcagagg ccaacacggt aactagtttt gaggtggctc ttgagaaact catgcggccc
    28801 gatcagtctc agtttgtctc gattcctttc gtaacgcccg agaaaaaagg aggcctgatc
    28861 attggattgc gttgtgagac tgccaccaca ttaaccgaca cgtctccgcc taacgaaaac
    28921 gcgtctcaag atgccaagac aaatgcctcg ccaacggaaa ggagagcgat ggcgggggac
    28981 caccgtagtc cccacgccaa ccgcgtcctg ccactgttga cccacagttt gctacgcatt
    29041 cgactgcccc tcgaggtgac actggctcgg actcgcaaac cgctcttaga gctgctccag
    29101 ttgtgccccg gtgcaattat ccagtttgac aaatcttgcg aggaaccgct tgagctttcc
    29161 atcaacgggc atgtcatcgc ccggggtgag gccgtgaagg tgggtgacaa atttggactc
    29221 cgtatcctcg agatgacacc gcctccggag cgctacatcg tgttagggcc tcgcaccgcg
    29281 aaaaaagaaa tcaagcaaaa atagctgcac tccagagatc gatttcaact tttgcttggg
    29341 ccaatggact tatttccgta ggcacgcctt cttaggcgcg ccacgcaggg gcctttcttt
    29401 ggttggaaac ggcattatcc gcctgcaaca tcacgtgatc caggttctgg ttcctcgacg
    29461 ccggtccggt aggataaaat ctatcaaatg gttgatcggg acgcttaaca tggggaaatc
    29521 gcacaacacg gagggcgccg gccctctgcg gcatgggcta ttgccttagg aaagcgggca
    29581 gaagtcgagt cacgactgta ccccaagagt aggcagggta tgatcatccg agagaggtgt
    29641 tgggaatgat ctttatcctc gataattacg attcgttcac ctacaatctc gtgcaacgac
    29701 tgggagagct tgacaactcg ttggaaatcg tggtccggcg taacgaccag gttacagtgg
    29761 aagaaattga gcggatgtcg ccggatcgta tcatcatctc tcctggacca tgcacgccaa
    29821 aagaagcggg aatctcggta gaatgtgtgc gatattttgc tggccgggtt ccattgcttg
    29881 gcgtgtgctt gggacaccaa tccattggtg aagcctttgg ggcatccatc gttcgggcga
    29941 aacgcctcat gcacggcaag accgacacaa tctttcacaa cgggtgcgag ctcttcgaag
    30001 gattgcccaa tcctttcatc gcgactcgct accacagcct ggtcatcgat ccgccatcct
    30061 tgccggactg tttcgaggtt tgctcatggt cttttttgcc ggacggtgaa aaggagatca
    30121 tggggatccg ccacaagact tttcccttgt acggacttca atttcatccg gagagcttcc
    30181 ttacagagca tggcccaaca attctggcaa actttttgaa actgccgagt ttggcgggtc
    30241 gttcctgact ttccacttgc attttgaccc ggaaatgtca gtcagggtcg tgaatgaaat
    30301 gaagcgcgtc aaagtaagag gggatcgcga cacaaaatca taccgagggg atgggtcagc
    30361 tgactcgccg gacggcaccc aatcgattca tgaaagagga ttatcacgac ttttgtcgca
    30421 acccattatt ttggccatcg ccggagcagc ccttctttgg gcagcgttac caccgcttga
    30481 ctggtggccg ctcgcctgga tcgccgcagt gccctggttg attttgatcg acagcccaaa
    30541 gacgtggacg aggcggcaat acattcagtg ggggattgtt ggtttcttct tctggatggc
    30601 ggcacttcac tggctccgtc tgccgcactg ggcaacgagc ttcggttggg tggctctgtc
    30661 tttctatctc ggctggtacc tgcccgcctt tatcggcctt tcgcggatag gcgttcggct
    30721 actacgactt ccgttgaatg ttgtggccgc aacagtttgg gcagggctag aagtcgtgcg
    30781 tgcccatttg cttaccggtt ttaccatggc gagtttgtcc cacacgcagt accgctggat
    30841 ttcacttctc cagatagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
    30961 ctctgcttta tggccagtgg aggctatcgc agtttaaaac ggggcaggtt aaccggtctg
    31021 ttcgcgtggc gttgattcaa gggtctgaag acatccgcct ggaagacagc gaggaaaagc
    31081 gggagcggat tcatcggcat tacctggact tggcatttca agcagcccgt agtcatcgcg
    31141 atctcgatct catggtttgg ccagaaacag tatatggcgg cttcttgatt gatcatgatg
    31201 atagcccggc agtacccgca acatgggctg atagtgatat gacgccagag cgatttagag
    31261 agcaaatcgg caaggcacgg gagcattccc aacggtcctt ggagacactc gcgcagcatt
    31321 tacgatgtcc cctgttgata ggagttgaga ctgtctcata cacaagccag gggcctcgca
    31381 tcttcaattc tgcggcgtac gtcgctctgt gtgagaagat aaatgcgtca caaggcggac
    31441 gggagcgatt ttcggacggt actgacgtca aagaagcaaa tgaggaaagt gggaaaaagg
    31501 gagattcgac cggccagcgg tactgctatt tcggccgata cgataagatg catctggtca
    31561 tgttcggcga atatgtgccg tttgccaacg tcatacctct ccttcaacgc ctgactcctt
    31621 taagcacaag cacgactccg ggaaagatac cggtcgcgtt tcctgtcggt tcgctgctcc
    31681 tatcaccgaa catctgttac gagagtgtca tacctcacct tattcggaac caaatattga
    31741 ccttgcgggc ccaggggcaa gaaccggaca tcctggtcaa tttgaccaat gatggatggt
    31801 tttggggttc gagcgaactg gatatgcacc tgatttgcgg tgtgttccgt gccctagaaa
    31861 cacgtaaacc ctttctgatt gcggccaaca cgggattttc agcccatatc gggcctgatg
    31921 gccgtattta ccagcaaggt ccccgccgcg atgtcgccat tctcgtggcc gatgttacgc
    31981 gcagtccctt gcgcagcctg tatttggact atggcatcat tcccaatacg ctcttggttt
    32041 cttttatcgt gctactcgtc attgctaagt gcctgtccgc cagtagaact ttccaaagga
    32101 aaattacgtt tcgtcgatcg gcttgagtgt ggtaatccat aaaaaaggct taccggcctc
    32161 atcgcagcgg atttcgccct tgagacgccc ttgaaacaga ctacgtttga taatctgact
    32221 gtaggtcgtc ttggtttgag gaatattgac gagggctttg tcaatttgga tcccaaagcg
    32281 ggccagggca gggtgatcca gtaaatacat tagactaaga cgttgtgtaa ccgactgaag
    32341 gacgtctttt acgggcacat cctgaatgtt aacggtcagc gtctccaata gctttggcac
    32401 aaccttcgcc tcagactcct ctggtgccca acccaccggc caggccgtga gattcggatg
    32461 accaatgaca atttgatagc gaatcccatt gactgtgctc tggggtacca aggcaagacc
    32521 atcatagcga aggatatagg cgagtgcggt tcccgtcgtt aacccttgca gttcctcctc
    32581 ccaaagatca cgtccaagcc tttggcgggc ctgcgggtcg aagactgttg gataagatag
    32641 gctggagact aaggtttcga caagttcggc ccgaggacgg ccctttgtcg atgcttggac
    32701 gggccgggac atatcctgcc gtacctgctc aaactgcacg atattgaggc cgaaggcaac
    32761 ttgtttcggg cgcgattgag gaggtccttg atgccgcaag tcgttgatcc attgctttaa
    32821 tcgatcaatt tggcgaatcg taaaacgcgc gtcgggaaga agaagctcat tttgggcgtt
    32881 tacgacacct agcacttggt aagtgggtac cgactcagat ggaatctcct gaatgtttgg
    32941 cgtatcgttg ggataagccg ttctgacccg aagattttcg acgcccactt cactcaacgc
    33001 tttggcccaa tcgtgatgcg tcaaaggcgg aagacgtggg tcaacagcaa cttccaattg
    33061 aatgcgtggc tcaatggcct gggcgcgagc cacatcgcaa aggagagaaa aaaagccaat
    33121 gaggccaagc aatgcttgga aaaccactct caggttttgt gacaccgatt ggttaagacc
    33181 cgtccgcgtt ttcacgactg gttctccttt gttttccgtg ccttgcgtaa ccgtggcggc
    33241 gaagaactgc cttcaggcaa agagctcgtg cttgcgtcgg ggagatccga gatcttctta
    33301 tttgctcggc ccagccgctc acgaatgact tcaagccgtg ctttcaattc cttctcgaaa
    33361 cctcgatcac tagggacata atatgttttg tcaactccca aatagtcctg ctccgcaatg
    33421 gcatcaggct cttcctggga atacacatat ccctggccgt gccccagacg ttttgcaccg
    33481 ggataattgg cgtctcgtaa atgaacgggt acgggcagga cacgaccgct gcgaatgtct
    33541 tcgagggcct catcaatcgc tttaatcacg gcattcgact tgggggcaca agccaaataa
    33601 atcactacct gggccaggtt gagacgacat tccggcaaac caatcatctc acaggcgtgc
    33661 gcagccgcaa cggccaaggg cagggcggtc ggatcggcgt tcccaatatc ttcgctggca
    33721 aggatcatga gacggcgggc aatgaagcgg acgtcttcac ccgcctccag cattttggcg
    33781 agccaatagg tcgctgcatc cgggtcactt ccacgaatgc ttttgatcag ggcgctgatc
    33841 gtgtcataat gagcatcacc ttgccggtcg tagacgatcg ctttccgctg gagcgagtcc
    33901 tccatcaact ggcgggtcaa acgaagggga aatgtctcag aagaaagaac ggccacctct
    33961 agggccgaca aagctcgtcg tgcatcaccc tcgcaggctt gggccaaaaa ctccaatgcc
    34021 tcctcctcca tgatcacctc gtagttgcca aggcctcgct ccctatcggt gagtgcccta
    34081 cgcacgatca ccttgatgtg ttctggtgtt agcggcaaga actcaaagac ccggcttcga
    34141 ctgacaaggg cgctggtcat ggagaagaaa ggattctccg tggtggcacc gacgaaaacg
    34201 agtccacctt cctcgacgtg gggtaatagc acatcctgtt gagcccggtt gaaacgatgg
    34261 atttcgtcga tgaaaaggac ggttttttgt cccgacgcca aaagacgacg ggcggcccca
    34321 tcaataattt cccgtagctc cttgacgccc gatgttacag cactgatctg ttggaagaca
    34381 ctcttcgttt ccgtagccag aatacgtgcc aacgtcgttt tgccggtccc cggtggaccg
    34441 taaaaaatga cggagccgag gcgatccgca cgaatgaggc ggcgaaggat tttcccctcc
    34501 ccaaggaaat gctcttgacc aacgaactca tccagtgtcc gcgggcgcat ccgggccgcg
    34561 agcggttgca cctcgcgtcg ccgttgttca tcttctttct cgaacaagga catgggaaaa
    34621 ccgccttaga tcacaagcca cgctgtcttc cggatgccca ttgatcccgg atggaaacgg
    34681 agttactcca tccaccattc cggcgccgct tgtccttccc tcgtgtcact cgagccatgc
    34741 atcgtatggc tggccatcgg gcaaataaat ctccaaggga cacgcctggc agcgggggcg
    34801 cttcttgcaa taatccttcc caagccgaac taagagggca tgatattcat tgtaaagggc
    34861 agcatcgggt tcgagttgac tctcgaagaa ttctttcaac gtgtgatagt cagcttctgg
    34921 atcgacccag ccgtgccggc tgaagatacg atgtgtgtat gcatcaacaa caaatgttgg
    34981 cagattaccg gcgtacaaga taattgaatc tgcggtctcc ggtcctatgc cattaatttc
    35041 cagcaattct cggcggagtt ggtcaatggg cagccgaaac atttgttcca gcgacccatc
    35101 atgccttttc agaagataac gcaaaacgtt ctttaaccgt cgggctttga tccgataata
    35161 accgcacggc ctgatgagtt cctccaactc ggcgtccttt agccgataaa gcctcttggg
    35221 acacatgagg ccttcctctc gtaggttgcg gatcgctttt tcgacattag cccaggccgt
    35281 gttctggacc aagatggcgc cgacgaggac ctcaaatggt gtctctccag gccaccagtt
    35341 ctggggccca aaggcgttgt acaatcggtc gtaaatctct ttcaatgaag gcatcggact
    35401 cataaatggc gcaccatgga ctcgagtgca gaacactcga gacgcatatt tctcgtcggt
    35461 tctctcaagg tgacaccggg gcgtgaacgc tgatcactct gcctggacat aaccactaat
    35521 tcacaacata gcggattgct gagaggtaag agaagtggtc ccggatcaca accagagacc
    35581 ttgaagggtc aagacaacat cgacggcacg accaacgacg cggtcaatgc ctccggtggt
    35641 ccgggcagtc actttgggaa tacgggacaa tgtgactacg ctggtgggat aacggaagac
    35701 aatctgatct gaatcaatca cgagctcatc gttgcccgtc gaatcgagaa gtcgggcggt
    35761 gtcgcagaga tcgaggcccc ctagggcaaa aaccctttca aaggcctttg cccgacgata
    35821 gctatcctcg gaggacactg tcgtttggtc gggcctcacg acccaggcgt tgatgttcgg
    35881 tccgccatcc aggtaggcca catcgatccc ccctgccgtt gagtagccat gcacggtcgc
    35941 gaagttatac acacggttga tgaatcccac tcctgacaag tatgattctc gcggttggct
    36001 cacaaactca tcattgcccg gggaatccca aagttcggca atgtcggtgc ctccagcgga
    36061 agactttccc gtaatttcct cgaaattgag cagatttatc tggtatccac tcccagtcat
    36121 ttcggctttt cttggccaaa cgcgcaagtg atcatccccg ggtgaatcgt aaagtaaggc
    36181 tgtgtcccaa cctccgctag cgttcgccgt gatggaagcg aatttttgga tcctgatttg
    36241 agtgcttggc atcgtcatat ccgcccagcg aggtcccgcc acgaaggtat caattccctc
    36301 ggagcccgta agtgtcgcgg cgtctctccc accattgccg ccagaagctt ggaccaattc
    36361 aaagaacttg gcacgcagaa agtagttctc acccctcaag ctagtccaat ctggccttcc
    36421 cacaagcgtg tcgttcccgg cggaaccaaa tagttgcgcc gtatcgtagc cgcccgcagt
    36481 gctgtaaccg tggcagtatc gaaatccttt ggcgcggtgg taatatcctt cacccgacat
    36541 cacaccctgt tcagggcgag ccacaaacct gtcgtcgcct gcagagtcgt aaaggtaggc
    36601 tatatcatca ccaccaaggc ggctgtatcc gtgaacgatt gagaaattct caaccacagt
    36661 cagaaagccg tttccagaga actccgcgta acccggccgg cagatgaaga gatcactacc
    36721 aggggaatca ctcatgtgag ctactgacgt accgctccct gcccgtacgc gaatggtttc
    36781 ggagttattc gcccggacct gccaaccctc cacggaaacc gtggcatagc ccggggacga
    36841 tgttagcgtg gcgtcacccc tcggcaattg aattgcgatg ttgtcgtaac cgtcgtaagt
    36901 gtcgatatat atggtacgtc ttgtccccag ttcggtagcg gagaaatcgt agctcacgcc
    36961 gttcaccgtt acgctaactc catcggtgag taggacagtg acttggtcag ctccttctgt
    37021 tccgtggatg gcgaggccgt catccgaaat ccccagtaag ttgaccagcc gaaacctctg
    37081 gtcaccctga aacccactaa aaaccagaaa atagtcctcg ttcgctccga cctgccaatc
    37141 ccatcggccg ttcgtagttg cggtcatctc gccagacaac agtcggcctt gggcgctata
    37201 cacaaagact tgcccacctg ctgcctcagg agatgacgcc agtaacagcg acacaattcc
    37261 ctcatgtcgc gtggaaaaac gatagagtga ttgtggctgc ccgccaattt gtacaattat
    37321 atcagtgaca tagcccagat tgataatcga tgaggcagat ggacttgcaa aattgacgcc
    37381 ggtgaccgtt tggttgcggg ccacgctcac cgttcggccg gtattggcaa acggcggcaa
    37441 cgattcctga gatggaagtt cgtaacacac cgtgtatgtt cccgcgggca gaccagaaaa
    37501 gacgtagcta ccgtccgcct gggtcacgac ataaggttcg tcgtacacaa acgttagtga
    37561 ccaatcattg aggacgccag tgtcccagta cattgtgtct cggacctcaa gggtccagac
    37621 accgtcggcg tcttcgccgg tgaacaggga caagggttct tcaggccgga aggaccccgt
    37681 aaacggtgcg actccttgcc cgatgagctg gggagcggaa tcatcgaaga ctgtgccgac
    37741 aaaattatcc cctgacccac cgacattcga ggcaagcctg acacggcgtc cggaggggct
    37801 gatgagggtc aacaacaggt cggcatcgta ggtgtgggta atattaaccg aaacaagcag
    37861 tgtgtgaact ttacccaccc cagaaaccgt taacctggag agtgttgttc cgtagtccac
    37921 cagggtttgg ggcgtcgatt gtgtgaatgt cgttgctccc tgatcgcgtc ggccatcctg
    37981 gtcaaggtca agataaacaa tggcgcccgc caccccgggc tctccgatat cgaacgatcc
    38041 attggcgtta aggtcttggt aaacgtaacc ggcaatctgt gagttacctg gcggttgcca
    38101 gtcatgggtc tggataacca gttcgggatc accgaagagc gtgcaactga ggtgaatcca
    38161 gcgataggca ccaccagcgg tcaggcggaa aagattatca atcttcgatt cgataaaggc
    38221 ttcacccaag cgggttttat gttcattgaa gatggcctta aagaatgcca aggcataatc
    38281 gtgactccca ccggggctgg ttcccggcac ataccaaccg taccgagtgt tcataataac
    38341 ggcgaccgca cctgccgggg caatgacgtg ttgctcggcc accgcaatat cgcgagtgtc
    38401 aaaggaaccg gcgtcacagc cctgcgaata cataatgtaa ggaaacgcgt tctgcagcag
    38461 ggcaacttgc gaggtcgtca tcctcgcgac gtaacccgaa ttagcatgtc ccaaatgatg
    38521 cacaatattc ggtgaagcat taaggctcgc tataacctgc gacgtcgtcc aggccgaagt
    38581 ggccccatca tagagtgtcg tgatgttcca gttcgagggg atcgcctgtt gccggatgat
    38641 ttcgttagaa gtgcttccct gagtgatgct gtcgagtttt tcgcctagga gcaaggcact
    38701 ttctagattc ggatgagcca cattggtata ggtgagggtc ttggtcacga agttgcgtgc
    38761 ctcaacaact gaactcaccg gcgcacgccc cacaaagact tcggggacta ggtcaatgtc
    38821 ggccccgccc agaccatctg taggttctcc ccaaaggttg tcaccgtctg cattccatgg
    38881 gccatccaag caggcataat acatatctgt ggcaaggttg ctgtcgctta tgttgcctac
    38941 agtgacatgc acaccgcggg cagggatgat ctcggaatca ccggccaaaa ggacccaacg
    39001 ggtcccatgc cgcaaatagg aatcacgaat gaaatcccga atgcgatcgg ccaagtcgcc
    39061 ggtttccgtg ccagtgtagt tttgggcgat ccattcggtg gttaccagtc gggctgtaat
    39121 tccccgtctt accttgtcct gaacaagggg caagaactcc gaactcaaac tagcgtttgt
    39181 aatgacaacg tattcatata aatccgtcga tgtcggggtg aggatctggt aggtttgaag
    39241 cacttcagga tttgccacca gcccggccaa ttggctagct agttgctgat ccacaaccgg
    39301 gagcgtttct cccgcgatgt tgttttgtaa gctcagctca aactcgatct cttcgtagta
    39361 agacagggtc gaggtggctt ggtcatacac caccggggta actgccacac tcgccaggga
    39421 gtaaccgccc caggaaaagg tcttgaaatg cacagtgttt tcgagtgtga gtgatcgatc
    39481 gagaacctct gtccacaacg ctcccggcaa ctcatcagaa tcaaacggca caggatttgg
    39541 cgcaacgacg agttcggcac cggaggcaag gacaacctgg ttacccgtcg cagcgatccg
    39601 tacgttggca atttgtgtcc ctttcggcaa gacaagtagg gacgaggaaa cggggacgac
    39661 cgggtcaccc gtagataacc acaaatccga cccatccaac accacaatgt taccggcgcc
    39721 aacggcttgt acctgtggaa tgccggagac cgcgacgcgg ccgtaagctc gccaataacc
    39781 ggtctcagtt tgggaaaacg tcgtccaatc aatatggatt ggcgagactg tcgaggaggg
    39841 ggtagtgggg cccgtttcag caagtcccat cgataagaga ggatcaaggg gtgtaaccga
    39901 taggagctca cgcgattcga gctgttcgac agctatgcgg aggttattcg atttccgcga
    39961 acagcctttt cgcacgcaac gtccaggggc ggaaggatgg ttatgagatt ttgtcctcag
    40021 agtggtacgt ccgcccgtcc cacttgcgca gccatccccc ctgacaagtg caaaccgact
    40081 catggaatac ctcaccaaag ttgctactca tcggtgtttg tttgcgcatc gcgctgtgca
    40141 ggcaactagg ttgcgccata cacgggacaa gagtatcaat cacctgcgac gataagccga
    40201 ccagacgagt ctctttcgtc tcacccacga gcgataagtc gtgaccgcgg ctcgtcactc
    40261 aaactatagg ttgcaagttt tgcggtcagg ggaaaacttg aggacttaac ggacgcggaa
    40321 aaggatggtt ctcccccgaa taggggtacg gcctgacaat ttttccgacg gtataagtta
    40381 tgccggtcgg cagatcgaag ggtatattcg gccaaacacg atccggcccg ccattcatcc
    40441 ccagaagaac gctctgagcg atggggcgtt tatcgcggtg gaagccgact aattagcgtt
    40501 ggggagcacc tatcccgttt tttgctgtct gagcctgtcg atcacgacag ccgcaatgat
    40561 gatcacaccg atgaccgctt cttgcaccca cttgggccaa cccatctgtt ggccgcccat
    40621 ataaagaatt gtgatgatga gggcaccgat gacgcttccc aagatggagc cctgaccgcc
    40681 catgaggctc ccaccgccaa tgacaaccgc agcgatgacg aaaagctcgt aggccacagc
    40741 cgaagttggc tggccgatgc cgccgatgaa ggaaaactgc ataagccctg ccagcccacc
    40801 aaaaaaaccc gccagcgtgt aggtcaccac cttgactcgg ccgacgggga ccccgcaaag
    40861 ccgtgccgtt tcctcgtttg agccaatggc gtacatgtat cgccccaatc gcgtgtaccg
    40921 aaggaggacc gccgcaaata tcgctgtggc aataaggatc cacacccccg gagggagaat
    40981 ccacatccgc cagtgaacat cctgggacgt gagcgtcgga tccataagct gcgtaagcca
    41041 ggtgtcttca tgaggataga tgtctttctc ctcggcaagc ccctttgcca gaccgcgaaa
    41101 gatgcccatt gtgcccaggg tcacaataaa tggaacgagc ctcagcctaa caatggtcac
    41161 gccgttcaca aatcccgcca aggtccctac gccaataccg gcgagcaggg caatccacgg
    41221 caaaacgaca ggatagacgc gaatgagata gacggtctcc attcccccat cggctgtcgg
    41281 gaccaaatag tggtaatcca gaatccatgc aacagtcacg agactaagag cgatgatcga
    41341 tccaatagaa agatcgatcc cggcagcaat gatcaccatc gtcataccaa gggaggccgt
    41401 ggcatagacg gcactctgcc gaaggatgtt ttcgaggttg cggggcgaat aaaattttcc
    41461 cccttccacc aaaatggcaa aaaataggaa gaccactata agtgccgtaa atcggcccag
    41521 agtgctcaaa aaggaagcat tccacctttc actttgccgc agactcgtcg cacgcttttt
    41581 cggtgtctcg ctcattggtg ttcggcaagt tcggaagttt gaccagttgc tgcaagcatc
    41641 agtttatgtt ctgtccaatc acctacgggg gcacaggcca caagttgtcc ccgatacatg
    41701 acggcaatcc gatcgcacaa tccgagaagc tccggtaagt aactggatac cattaggacc
    41761 gccctcggac gtgtaccgta ttccggtgat ccctccgcag cctgagcaat tagacgatag
    41821 atttgggcct tggaccctac atcgatacct cgtgttggct cgtccagaat caagacatcc
    41881 acatttgcgt agagtagtct cgacaaggcc accttctgct gcgtgccccc agagagtgcc
    41941 cagaccggct gacccggtcc ttgacacttg atggccaaac tttgaatcca tcgttccgcc
    42001 atctgcctct cccgggaagg gagaatcaat ccccatggtc ctaaataatc caagcggggt
    42061 aatgtcatat tagccgagat tgacagcccc atcgccaatc cttcccgctt gcggtcttcg
    42121 ctcatgaatc ccaccccttg gcgccaacgt tttcccgggt cggcccaacc agaaaggact
    42181 cccacccgaa ccaatccttg cgtgatgggt tgtaaaccga aaattgcccg caatagttcc
    42241 gttcggccgg atccaatcag tcctgcaatt ccaacgattt cacctcggtg gagtgttaaa
    42301 ctgacatctc ttggcttatc aatcccagtt agacccctga tttccaaaac cacctctccg
    42361 cgcgttcgcg tccgccgcgg atacaactcg gtgatctcgc ggcccaccat catacggact
    42421 atctggtcga cgggcgtgct tgcggtcgag ccggtaccaa cagaatgccc atcccgcaat
    42481 accgtgaacc gatcactgat ctgtttcacc tcctcaagga agtgagaaat ataaatgaca
    42541 gacagcccac gttctcgcag acgttttacc aggtcgaaga gccgaacaac atcatcctgg
    42601 gtcaggctgc ttgtcggttc atcgaggaca acgaccctgg cctgagtaac cagtgcgcgg
    42661 gcaatttcaa ccatctgctg aagacccggc ggcaacgccc gcacgggtac cgaaagtggt
    42721 aaatcactta aacctagttg ggaaagcgcc tcttcagcac ggcgtttcat ttgcccccag
    42781 cggataaacc caccgcgatg aggttccatg ccgaggagga tattctccat gaccgaaaga
    42841 tgtggggcca gggacaattc ctgatagatc atcgcaatcc catgccgccg tgcatccagc
    42901 ggatgaagcg gggcaaaggg ttcaccgtcc agccacatag ttccccgatc tggcggtatc
    42961 gccccggaga ggattttcat cagggtgctc ttgcccgccc cattctcacc caccaaggca
    43021 tggacctctc ccggtaggac ctcaaggctc acattctcaa gggcgattgt ggcaccaaac
    43081 cgcttgcaga tgccatccat gcgaagacgt ggcacctcca cagagggagt gagggaagct
    43141 gctatcggag ttatcttggg tcgcaccata gcgttttgcc ggccgcaggt tgtgtcattg
    43201 cgaccagtgc tatttcgtct gaattgtcct ggctcgatca aatgtcacat acgctcatct
    43261 tgcaacgacg caggatgacc tgtcactgac cgcttggatc atttgtcctt aagacccacc
    43321 aattgcctta tcgcaggatc actttctagg cgactgcgag tgacgacctc aacacccgtg
    43381 tcgatgtact tgggaacttg ctgcccccgc aagtatgaga caagtgtctc taccgccaat
    43441 cgccccatct ttcgcggatt ctgcgcgatt aaggcatcaa tgtcaccttg ttggatggcc
    43501 tctacaaggc gaggggtaaa atcaaagccg atgaacttgg cattgacttt tacgccactt
    43561 tttcgaaggt catccaaagc ggccagcatt cccaacgtgg aacggtcgtt gcaagcaaag
    43621 atcccgtcca gttgaagttc gttattctta acgaagcctt ccaaggtgtt agccgccgtt
    43681 ttcttgcacc cagctacggt ggcgtcctcg ggataggggt cggcaacaat ctcgaacccc
    43741 gccgcttttg ccgtttcgat gaagccttct gcccgcgcct cggtgcttcc ggtcccttga
    43801 acaaagcgca aaaccatcac ccgccctttt ttctccccca aaagctctgc caggtgctga
    43861 gccgccaaaa ctcccccttg tttgttattg gttgccacaa aactgacgtg ggcatcaccg
    43921 tcgatttcag agtcaaagat caccaccgga atcccagcct gaactgcccg ttcgacagtg
    43981 cgacgctggg ccgtgcgatt caacggcgcc aaggcaatac catcgacctg caagctaatc
    44041 atgttctcga taatcttgtt ttgctcggcg agttctgtct ctgtcaacgg tccttcccat
    44101 ttcatctgaa cgtttaatct ctcggcggcc tcccgtgccc cttcttcaac ggtttcccaa
    44161 aactcagccc cagtactctt gggaatgacg gctatcgtta actttttctc gcgagctatc
    44221 ccctcaccag tctgcgagcg gcaactgagc gacagagacg tgagaatcag aaaactgacc
    44281 agcatcccat gcgtgtacgt ccgtagttgc ataaggtaat gacctcctcg aatccacttg
    44341 ccgtcgtgtt tcagcgaaac ccttgactgt ccagtttatc ctgacctatt gtcacctgaa
    44401 agcgatttgt tattgaagat ggtcctcgcc cgtgccaatc caaagttcct ttcacgctcg
    44461 atttgatccc aagtggatcg cacttgaaaa tcatctcacc cacaatcttt gtaagaatcc
    44521 cttgccccct tatgcggtta tcttttggtg gagggacact gtgagcggtt atcccaaccg
    44581 ataaccacct atcattcgtt gtaaagtcgg cagaagacag tagtgggttt gtcgcattcc
    44641 acaacgaact ccgttgcatt ctcagctaac gtctttgccg aaaagagttc ttcgatcctc
    44701 gtacatcgct ggggcaaacg gatgggatac cgcccgggga ttagcagcga gaatctcctg
    44761 gaattgcctg tctaataagg caaaatcata catgttcggc ccaagccata tcggtggata
    44821 gggactatat gggacaccca ccgacgacaa ggtgtttgcc ggaaaacaag tcacggtatg
    44881 aattcctaat tcgagactat cccacaacct cccctcgccc gttcggcggg agaggggccg
    44941 ggggtgaggg tggctcgaat cgctgctcgt tttctcgatc gcctccgacg atgccttacc
    45001 ctcaccccaa ccctctccca aagggagagg gaactttggg ataggctctt caccaaatcg
    45061 tttggccgtc gcgaggtctc gcaggtatga atgaaatgcc atccagcgaa ccggcgggtg
    45121 ctgtgtccgc tgcgttggtt cgtcactaca taccggggta gcgcaaacca gaatgggtaa
    45181 gaaccagaag atcgcccacc atgccctgca gggagatgtt accattggcc gtgctccatt
    45241 agcttcgaac gtcttttctg ttggccactt aaacccatga aaaacagtgg agtaatggtc
    45301 caaattcttt accacaagtc gtcatgttcg acacgttact tagctacggt tgagctgaag
    45361 cgcggttggc gtctttttgg agcttgttgc cagcgggccg ggcacggcga gggccttcaa
    45421 aaatgggagc aatgttcttc tggttccatg tctcgtattg acggcgaatt tccagaacaa
    45481 tttcgggatg ctggtgactg acattctttg tttctgaaat atcctctccg atgttgtaaa
    45541 gttcttcggc ctgctgccca accttcacaa gtttccaatc gccgctgcgg actgcccaat
    45601 tcgtgcctcc tcctgttcgc cagaagagcg acttgtgcgg ttgatcctga cgctgccctt
    45661 gcaagtacgg caagagattt actccatcga ccgccgattc cgtaggaaat gccgtgtcgg
    45721 ccaccgcaac gattgtggca ggaatgtcca aggaaatcac gggctgctca taggtggttc
    45781 ccgagggaag agtccccgtc cagcgaatga cgaacggtac ccgaatgccg ccttcataga
    45841 cagaaccttt tgcaccacgt aatggggcat tgctggcgtg tgtgacctct accgggccgc
    45901 cattgtcgct caaaaagaag acaagcgttc gttcctccag tcccagttct tttagcgcgg
    45961 cacagacccg gcccactcct tcatccaggg cagccaccat ggcggcgtaa cttcggcgtg
    46021 tagggtcttc gatatggcta acacgttgca gccagctttc cggcgcctgc aacggcgtat
    46081 gaggggcgtt ataggctaag tatagaaaga acggcacttg gtgatgtttg cggataaatg
    46141 ccacggcctc cgatgtaagg acgtccgtca aatacccctc gaatgcctca ggctcggtat
    46201 ttcgctgaag gggaatgaaa tactcgcggt tttcgcccgg cggtccgaca cggaaataat
    46261 catgtccccc gccaatcaat ccgaaatact catgaaatcc gcgccgtaaa ggatgaaaat
    46321 gaggtgccgc tcccaaatgc catttcccaa cggctcccgt cacataaccg gcccggctta
    46381 atacgtccgc caccgtgacc tgagatatgg gcaagcccgc caccggatca ttgggatcat
    46441 acttgggatt attctcgtgt ccaaaccgct gctggtaccg tgccgtcatc agtcctgcac
    46501 gtgtcggact gcaaaaagga tgtgaaacat aaccctgggt gcaacgcatc ccttcccggg
    46561 ccagagtgtc aagattaggt gtgggaatat cttgacaccc ttgaaaacca acatcggcat
    46621 aacccagatc atcagcaagg atgataacaa agttaggcag tttggaacgg gcgttttctg
    46681 aggactcggc tgtttgagca aagagcgaca atccccccgc ccaaaaaagt aacaagactt
    46741 tcagcgctaa atattgatga cccattattg gacctccatc ttgctcgcaa tagctgagga
    46801 tatgcagcct ggcgtcagtc caagcccctc cgatgccatt ttgtccgaca aacacgctag
    46861 ggattcctcg ccttcagggc taacatgccc tagttattgg aagggcgtta agagaagtca
    46921 agcccacggc ggtgtctcaa attgtgcagc tagaaaatcc cgagacttga gaaacggaag
    46981 accccacacc aggatagaag ataggatgag gcaaagcttc cacatgatgc gttttcaggt
    47041 tgagaaccat cggggtaact cgtgccattg atagggtctt cagcttgtcc cgatctgaca
    47101 ggtacccggc atgaaactat cactctggca tcatctttgg cggcccatca cgcggcagat
    47161 tttgggctcc atgattctta tctcggtctt gagcagcctc atcgcaagcc tgggcatggg
    47221 gctcatttgg agtgcacaaa tcaagaaccg ttaccgggaa gagcttcgtc gtgtctgtga
    47281 gatcatcaag cacgggggat ttcctctcca tcgcacggtc ctggcacaac tcagtggcct
    47341 ttctgggatg gagtttattt ccctatccac gagaggcgcg gttatcgaca gtacgctcaa
    47401 gctcagtaac gaggatgaag ggttgttggc ggcgttggtc gccaagcacg gtaatgcgga
    47461 ctcatcctct catccgaggt ctcttaacct tggaggaacg cattatcaaa tagtcatcgt
    47521 ctccgttctg gctgccagag gcggggctgc ccccattgat cgcgtgatcg ttcttcagga
    47581 ggaaacagcg ttgcttaggg aacttcaaca ggttgttctc cctaccttgt tagcaactgt
    47641 tgcagcagtt gtgattggct tccttttatc gattggaatt gcgcggcata tcagccaacc
    47701 tctggttagt ttgactgagc aaacgatcaa agcagcccac gacttgcaaa gtgacatcgc
    47761 gctacccaag accgacgatg aaacacacgc gttggcagag gctatccaac gactgattac
    47821 cgagcgtcgc ggctacgaac aaagtgttcg gcaagaggcc cgtttacagg ccgcgcatca
    47881 gttcgcgctg ggactcggac atcagttgcg gaatctctta acggccctaa ggatggccat
    47941 cgagctgcac gctgagcggt gccacgtcaa tcgaaatgat gaggatcttc ttgtcgcctt
    48001 gcggcaggtg agactaatcg attccgtgtt gcggcaattc ctttcattgc gtgcgttcgc
    48061 cgaattggag aaagaacctc tcgactatgc gcaggtgctc gaaagtgtgc tcgacctgct
    48121 tcggccagcc tttcagcacg ccggcgtgca gctccgcgtt gaaatgcccc aaaaaccatg
    48181 tgtggtcacg gggcatcgtg cctcgcttga acaattgacg acaaaccttg tcatgaatgc
    48241 tcttgaggcg gtccaagggg tacgtgatga gcctgtggtt actgtggccc tgcggcgttc
    48301 ggaggaaggt gtcatccaac ttgtggtcga agacaatggc ccgggaccgc ctgcaagtgt
    48361 ggcaaaggac ctgcttaaga agcccgtgac ctcgaaatca gacgggattg ggctcgggct
    48421 attcgtcgca cagcttatcg ccgaacaaca taatggaaga atcatttgga gaagagaaaa
    48481 cggtcgaacg caattcgtgt tcgagcttcc gaatcattct taacacgcca aaagatgggg
    48541 cttggagcgc cacggagttc gccagatatg ggtcatattc tcattgtgga tgatcaacca
    48601 gatatttgct ggggactgaa aaggatcgcc gaacagctcg gccatacggc tgaatcggtt
    48661 gccacagcag aggaggccct tagccgtgtc ttatcaacga accccgacgt cgttgtgatg
    48721 gatgtccggc tgccgggaat gacgggcctc gacgccatgc aagagctaaa gaaaaaaggt
    48781 tgggaaggcc cggttgtcgt aattaccgcc tatggagatg ttgaagtcgc cgtcgaggcg
    48841 gtccgccagg gcgcctttga gtacctgacg aagccgtttg acctaaaggt tgttgcgcga
    48901 gccatcgaac gggctgtggc atctcgtttg tctacaccga cagacgaaaa gaccggcgag
    48961 ttggaggaaa gaagttggcc gcaaatcgta ggggtctcgc cggcgatgca acaagtcttt
    49021 aagcaaattg ccatggttgc cccgaccgat gccgccgttc atatcgtagg tgaaagtgga
    49081 acgggaaagg aattggtggc gcggtccatc cacggtttta gccaccggcg gaagggtcca
    49141 ctcgttgccg cccacattgc ggccctgagc ccatcgcttg cggaaagcga gttgttcgga
    49201 cacgttcgtg gtgcgttcac gggtgcggac tacgaccatg tcgggctttt acaaagggca
    49261 cacgggggaa cgctgtttat cgatgaggtt gccgagattc cccttaattt gcaggtaaaa
    49321 ctgttacgtg ccattgaata tcaggaggtg acacctgtcg gggctgtccg tccgattcct
    49381 tgcgattttc gcatcatttc ggcaacgcac cgagatctca gaaaactcgt ggcggagggg
    49441 aagtttcgcc acgacttgta ttatcggttg atcacctttg agatccgcgt accaccttta
    49501 cgggaacgac cggaagacat agaaccacta acgaaattct tcctcaagga attgtgtcga
    49561 aggatggaga aaccggtccc ccattggacc ccagagttca tggctgcact tttgagtcgt
    49621 ccctggtggg gtaatgtcag agaactccga agtgctctgg aacacgcctt gatcctttgt
    49681 cgaggaggac tactccaccc cgacctgcta ccacctcccc ttccgcaggc atcctcgcaa
    49741 gttaccgctg ttgcgagtaa tttgcaggat tgcgtgcgac aatggacaca agatgtcctt
    49801 aagacgggcc aagcggtagg tcacattcac caggagtttc ttgatatcgt ggagcccgtg
    49861 ttattcgaga ccgtccttca gcactacggc ggccagtatg tgaatgccgc ccgcgccctg
    49921 ggaattcatc gagtgacact gcgccgcaag atggaccgtg tcaagagatc cgaatcaggc
    49981 caacaatggt ttcgctgacc ccattcctaa ctctccaacc gccccactca cccgagaaga
    50041 ccacggcgtt tacacaaaaa ggattccccg ccataatggt gggtttgaat cacaaagttg
    50101 agaatgacgc atgggtatag ccaggagtat taggagccac gttaatgagt tctgccgctg
    50161 agatcgagct gaaaccattt atccgcagca ttcccgattt tccaaagcca gggatcctgt
    50221 ttcgggacat cacaccgctt ttggcttcgc ctcaagcgat gaaggcgacc attcgctatc
    50281 tttccgaggc ggtccgcaat cgagatgtcc aaatgatcgc cgcggcagag gcgagggggt
    50341 ttttgtttgc ggcacctttg gcgatcgagc ttaatgtggg attggtaccc attcgcaagc
    50401 cgggcaaatt gccctctgat actatttccc acagttacac ccttgaatac ggcaccgata
    50461 ccttggagat gcataaggac gccatcgcac cgggtacccg ggtgctcgtt gttgatgacc
    50521 tgctagccac cgggggaaca gttgaagcat gttgccgatt gatcgaaaag gcgggaggca
    50581 tcgtggtggg gtgtgccttt gtcattgagt tgacggacct caagggtgcc gaacgtctgg
    50641 ctcgctatca tccggtcagt ctcatcaagt ttagtggaaa gtaatggatg atcagggatt
    50701 gggaaggagc cgtgactcag gtttggcagc gaagaaactc gccaaaagat cggggtgcgt
    50761 cccaaagtcc aaatgaatga cggaggcgac gactctgtca tttccgatga tgtcgtactc
    50821 aaatccaggt tcggctatcg cgggtgccaa aagcggctcc ggctcgaacc accatttggg
    50881 gcttcgataa ccccgcaaaa cggtccccct gctggccaac cacattggcc gagctacggt
    50941 caccgtgact ggtagcggct caccaaatcg cggcaattgg cgcccggcag ccggaaagag
    51001 accaacacca cggacaaact gccccggctg gatttccatc cactggcaca aataagcagc
    51061 tccggctcct tcggcgtaaa tccttccccc acgacgaaca tggttccgaa tcgcggcctt
    51121 caagcagtga ttgttcgcca aatcttcggc ataaggttcc ggttgtccac acccgagata
    51181 gataacgtcg gtatcttccg ggatgcgttc gtccttgacc ggagaaaaat ccacgatccg
    51241 tgccccgcag cgttccagcg attccaaggt ctccggaaaa taacacccaa aaacagcatc
    51301 cctggcaagg gcaacggtaa tgcggccccg ataatccaag accggccact cctcgatggt
    51361 aaatggccgt tgagcactcc gaaaaagaat cctctgaaac tggtggtcat tccaccaaag
    51421 ctgcgctggt tcatctgccc aaaccgaaaa tcccttaatc gggtcacatt tttccggagg
    51481 cctcggatag agaattccga ccaaaggtaa gccacagtaa acttcaaagt cgagcaaata
    51541 ttggagcggt gctatctctg gatcggcatc cacaaccacc acgccatcca tcgccttctc
    51601 actaagggga ccttcgccaa ggaactcatg ataaccttgc ccgcgaagga ttaccagctt
    51661 ggaaaggctt agccaatcac aaagggtacc gaggtggcca aaaaatgagg tggcgtcggg
    51721 cgcgccgcat acggaacgag accgaccttg tgatattacc ttggcggaac ctttctctgc
    51781 ttggaaacgg cacggacctc tcacatttcg aaacgaggcc ctttcatccg tcgtgtgctg
    51841 cccagtacca aagtgacacc caccgccccg ctcgtattcc cccaccacca cagccaagtc
    51901 cgcgccaacc acaccgcgaa tgaacgactc acaacagcgc gccggtgaca tgagccagct
    51961 atcgagccgc cgcggtgtcg cccccgacca ccgcttaagg ttttgaaatc cgaagaaact
    52021 cgcttgagac agaaaagtct gcacatgcag tccttgtgaa gtgagctcct ggaggaaaat
    52081 ccgcaagaat gaccatgggt ctgtttcctc gtcaatcgtt ccgatcgcca aacgtggtgt
    52141 tttaataggc gtcattagct acggccacac tcgtaaaagt tgcttaaata atctaaactt
    52201 aataggatct ctccctgctg gcatttttcg gggacaaccc aacacagtca aggcaatacg
    52261 acaaagcgaa tgcgactttt cgcccgctcc tctccccgtc gggtgagaaa aatggggttt
    52321 tcttgggagg cacgaaaggt gggttaaacc tctttcaggc gaagaatcgc ttgccgtgcg
    52381 cgttcaagga acaaatgctt tggctcgccc gattcaagcc acattggtgg tccgaatatg
    52441 acgcgcgtga gcagcggaac aggaagaaac tcccctcgcg gaagaatccg attcatgtta
    52501 tcgaggaaaa ccggcacgag ttcgaggtcg ggccggcgct tggcgagata atagaggcca
    52561 ctcttaaaag gcccaacttc agggccagac gacctgccac cttcagggaa gataataatc
    52621 gaatacttgt cgcccatcgc cgaaacgata tcgcttaccg gcgaatgatg taccttaatt
    52681 tcctggcggt caatgagaag ggcattgaat acctctacag agagatatct ccgcactgga
    52741 tttgcagtcc agtagtcttt ggcagcgatc ggacgcgtca cagccctgac ctgcggagga
    52801 agcgccgccc aaatgacgac agcatcgaga tggcttgtgt ggtttgcaaa atacacccgc
    52861 tggcaggtgt ctggctgaca atcgacccac cggacacttg caccgctcaa aacacgtgcc
    52921 gttagtgcaa ggataactcg taacgttgcc gcaaccatat tgccttgcca agcgaaatta
    52981 ttcgaaccca attggggtga tttctgatat ccgacttaca atcgaggccg ccataagaaa
    53041 tcgctaagac tcactttatt ttagcttgat ctgggccgtc gcccgactct gggccctacc
    53101 ggtgaaaacg tatcccgcta atcgactccc atttgctgag ccaggaaagg cggtttagat
    53161 taaatgtttt cgctgttgtc gtattttggc taagagtcga tccccaaaag tcctcctctc
    53221 cctcggggag agggttaggg cgagggcaat cgatcgccag agtcgatcga gaaaaccggc
    53281 agcgattcaa gccaccctca cccccgaccc ctctcccgcc gaccgggcga ggggagatta
    53341 cgggataggc tcaaagggcg atttgactct tttcgctaag cgaccgtcgc aaatcaacag
    53401 caagaaaaca ccacgcggaa aggaacccga aaaggaaaac acccaaaata gatctccggg
    53461 agattactta tgcaggtcct cgttgttggc ggtgctgggt atattggttc ccatgctgta
    53521 aaaatgctgc gtgagcaggg gcacaccccg attgtcgttg ataatcttac tcgcggccat
    53581 cggaaggccg taccgagcga tattcccttt taccagattt ccgcctgtga gatcgaacgt
    53641 ctttgcgaaa ttctgaagaa gcactcgatt gaatgtgtga tgcattttgc cgcacttgcc
    53701 tatgttggcg aatcagtgga aaaaccgctg atgtattacg agaacaatgt ttccgcgacg
    53761 attcggctct tgcaagcgat ggagactgtg ggcgttcaga aatttgtttt cagttccacc
    53821 tgcgccacct atggcgagcc aagcaccatc cccatcaccg aggcttgccc ccaaaaaccc
    53881 attaatccgt atggtcggtc caagctcatg gtggaacaga ttctggccga ctacgccgcg
    53941 agccatccag cgtttgctta tgccgcgctt cgatacttca atgtagcggg ggcttcacgt
    54001 gacggcactt tgggcgaaga tcatcgcccg gaaacgcacc tcatcccttc gattcttttg
    54061 acgttgctgg gtcgtcgtga caagttcgtt gtctttggga ctgattaccc cacgccggat
    54121 ggaacgtgca tccgggatta cattcacgtt gaagacttgt gcgccgccca tatcctggtg
    54181 atggagaagc tttgtcccgg gcaacctcgc atttacaatc ttggtatcgg gcgagggtac
    54241 tccgtcaagg aggtgattga cgctgtggaa cgagtgacgg gcaagagagt tcccgtcgag
    54301 cacggcccgc ggcgggcagg ggatccggcg atcctttacg cggacgcaac gagaattcga
    54361 aaagaattga actggaagcc ccaaatcaca acgattgaag aaatggtcga aaccgcttgg
    54421 agatggcatt cggcgcatcc ggaaggatat gtgagttagt ttttttgcgt cgaggcccca
    54481 tatactgccg agacgtattc gcaactgtgg aggtgtcata tgaaggcgac tattgcgttg
    54541 gttttcgcgg tcctgggctc ggtcatcgtt tatccggtct tggtttacgg tgacgagccc
    54601 cccaagtcca tcgaagagat tgtggcaccc ggaacagaac ctcaggttat cggctctggt
    54661 tacgggttct ccgaagggcc ggcagccgac ggtgaaggta atgtttactt ttctgatggc
    54721 aaaaatgatt ccatctacgt gtatcgtgtg gggcaaggtg tggaactatt cgttaacgat
    54781 agcaccgacg ccaatggcat gatgttcaat agcaaaggcg agctgtacgt ctgcgaggga
    54841 gcagcacgtc gcatcgtggc cttcgacgtc aaaaccaaga aaaaacgcgt cttggcaagc
    54901 gagttccagg gtacggcgtt caacgagccg aacgatctga cgatcgacca acaggatgga
    54961 ttctacttta cggatccgaa ttatcgtcac cgaggtcagc cctccctgat gaaggaggat
    55021 gtgtattacg tccgtgcaga cgggacggta tcgcgtgtct cgcaggtgtg tatcaaaccc
    55081 aatggaatcc tgctgacgcc ggacaacaag gtcctgtatc tcgctgacaa tgctgctggg
    55141 atcatttatc gatatgatgt tgttggtccg gggcaattag ctcacgaaac aaagtggatt
    55201 gagctgggtg cccatcctga cgggatgacg ctcgatgagt ttgggaatct gtatgtcgcc
    55261 tgcggtcgtg ccggaatcaa agtctatacc aagaacggtg aatacctggg cacgatatcc
    55321 gttccgtatg cctccaactg tgtctttggt ggtaaggact tccgcaccct ttttgtgact
    55381 tcggcggata aagtctatgc tattcccacg aaggtgcggg gaatcctacc gctggtcttg
    55441 cgcaaacaag agtaactgtt gtgtgacgat ggtaggctat gccggaggcc ggttggtcag
    55501 gtcggtctct ggactttttt ggtaatattc aagaaggcga cgaaactttt gcgacgggcg
    55561 ttttcgtctc ccacgccac
//
LOCUS       DAOSUT010000005        52538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_454_length_52538_cov_9.385477, whole genome shotgun sequence.
ACCESSION   DAOSUT010000005 DAOSUT010000000
VERSION     DAOSUT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 52538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52538
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_454_length_52538_cov_9.385477"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1066)
                     /locus_tag="PLD05_00920"
     CDS             complement(119..>1066)
                     /locus_tag="PLD05_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOP76014.1"
                     /translation="YNQLGEEELMERIRPESEEERAQIPTHMPYIVIIADEMADLMMT
                     AAKEVETHIIRLAQKSRAVGIHLVLATQKPTVDVITGLIKSNLPARICFEVASRVDSR
                     VVLDEMGAEKLLGNGDMLFLRPGTSTLIRGQGTYVSDDEINRVIDAVAVHEPEFVQEL
                     VQLKPSQPGDPNKPGLAARDELYEAAVDIVIREGRGSVSLLQRALGIGYGRAARLIDY
                     MEEDGIVGPYNGSQAREVLMSLEQWEAMRQNNGQATSQPASSRPATHSAPGNAGRSHR
                     IVLVSETGEDTPPPGPSASFGALPPRPESTPPAQPPTNV"
     assembly_gap    1067..1166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..2488)
                     /locus_tag="PLD05_00925"
     CDS             complement(<1167..2488)
                     /locus_tag="PLD05_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOP76015.1"
                     /translation="MASLRAELGRDLVALLLLGVVLFFGASLITFDPADSPSDLVFPP
                     HAQVTNVCGPVGATVAFYALEFLGIGAWYAVLSLSVLTIVLLLRQPLTRPNTRVFGWF
                     LSIFGLTGLADCVIPNWSPGPVVGPGGYVGVALRWILEQKLGFAGTVATLFAGFSIGF
                     LLCTELSLVRTVLTAVLWPLKGIVKGLGEVASGYGLGRPTSEAKAQDSAVRVKRPGEL
                     AEVPSGQAKDAAPQGSEPGETDTSHRGPLIKTPAPAKEHQPQHAETVQKPEANDRKSG
                     DLRIRKPTRLPKEELLDVIDSSTDERRHFNYKLPPLDLLLEPEPFHFEEQEKEVRRKA
                     QILEKTFADFGLHVRVVEIQTGPVIAQFEVELEAGLRLSKVTALADDLAIALRVPSVR
                     IVAPIPGKNTVGIEVPNSERQIVRLREVIEESHGKYQAMRIPIFLGKDV"
     gene            complement(2796..4187)
                     /gene="pgk"
                     /locus_tag="PLD05_00930"
     CDS             complement(2796..4187)
                     /gene="pgk"
                     /locus_tag="PLD05_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOP76016.1"
                     /translation="MALTKEKMEAWLRRILGVDKVTMGLLDYIAGIPRVKDLTDVPAG
                     TPVLVRGDLDCKPGAKVGEGDIRLRSMKETLLLGRDRGWIQVIFGHIGRDPQLSLEKV
                     WKRLSEILECEIVFIRDWFAPETLKIRQEVVDAIKAAKPGDIILLENTRKYDIERVLW
                     KAKPADIPNLVEKLTNVANEFAEKIAKVYVHEAFSAGSLDTSSVVVTAAMEKVALGEY
                     AYGEFSGPLADCLNAQLVISSGLKIDKLDDLEAMISRGKIRWVFTAGSLAMGLIKAAY
                     QLDGKDFCIGVAEDPAHKDQPYYIPPERIEQAKKMIASGRAQGIQFVMPVDFVLQDGR
                     VATTLSPGDQQFDVGPATSELFERKIGEFLDWAKTLKEPAVAFHNGVFGKFEDPKFEN
                     GTRRFMAQLKRMKDAGVRVYVGGGEGGTALEKYGQPDWVTHNFTAGGTVLNALGSEPV
                     PFLVALHMAANKK"
     gene            4543..6501
                     /locus_tag="PLD05_00935"
     CDS             4543..6501
                     /locus_tag="PLD05_00935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOP76017.1"
                     /translation="MSGKDSRVKRIFSRRSRWAVLFVAVAVVGATVAVRSLWGPNAAR
                     AGGPGILGAITGSRQAPGPTATAARTQPGPTTATPSGAAASSARDERPQVVAVVNREP
                     ITRDELRKECLRHYGEEVLESIVNKYLIMEECQRRGITVTAEEVDAEIVRMATRFGMP
                     VETWFKLLKEERGIKPDQYKRDIIWPMLALRKLAGDRLLISQEELRREFESRYGPAVK
                     ARMIVVSKKETAEAIRAEAVANPDQFGELAKKYSEDPASASLKGLVQPIRKHVGQKEI
                     EEVAFALKPGEISQVIPIANQFVILKCEEHLPPAKVRFEDVKGEIEELAKDSKMRAVA
                     HEIFRDLQSRAEIVNVMNDPQKRAQMPDVAALINNRPVRLEDLGEMCIDRHGEEVLEN
                     LISRKLIEQACRKRNIQVTEADVDAEIERAARENLPLLPDGRADVQRWMQLVTQQQGI
                     KPEVYRNSIVWPAVALKKLVGDNVSVSQEDLQKAFEANYGPRVRCRAIVLNNLRRAQQ
                     VWDKARSNPTEEYFADLAEEYSIDPATRALRGEIAPIQKHGGQPLLEQEAFSLKPGEI
                     SGIIQVAPETYVILYCLGQTKPVDVDLASVQEILKEDLKAKKVRLAMAELYEKIRSEA
                     TIDNYLAGTTQSPNLNRAAQRASSGDVR"
     gene            complement(6565..7416)
                     /locus_tag="PLD05_00940"
     CDS             complement(6565..7416)
                     /locus_tag="PLD05_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76018.1"
                     /translation="MSEEPDRPRVLEREEYIEQAYFFRALRERLDQGVSTQELIKTIA
                     QEILSTTKLPLALDFMVTELRFTGGFSTAMARLGHYFTAFQTYVIREAEREDGRLDFR
                     IALEILEKEAEYRAKGADPQGVFLYQFETLCRNRLGYDAGLEAMARDPIYDENWKDWI
                     LSLRRQVGLVDIADMIFVRSQYYQMLKGETGKPVLFGEKEGKIALANRRKDPLYLFSA
                     LSRHLGYPSVPRPRKEESVIQNYYAMLRRLEQLERRVKLLEEEHRGGIDLSQFYVKPE
                     DQNNAGK"
     gene            complement(7685..8143)
                     /locus_tag="PLD05_00945"
     CDS             complement(7685..8143)
                     /locus_tag="PLD05_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOP76019.1"
                     /translation="MNRGVRRAFTLIEVLIVVVIMAILAAVIIPQFAASTSDAKRSAL
                     EFNMHTLRSQIELYRIQHGQYPTISNNDLPQLYNATNLAGEIGTPGPNYPYGPYLANG
                     LPPNPYNNSNKVAPVSTPGQKPTGVVSGDYGWQYDQSTGAIWPNNPEYYQ"
     gene            8696..>9410
                     /locus_tag="PLD05_00950"
     CDS             8696..>9410
                     /locus_tag="PLD05_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76020.1"
                     /translation="MRTVWTVPLMAMLMIAVAAGKVDAGYCGAVRLALQPTGCALEPG
                     CGPQQCYTVLKPSRKLVFEPQQVTSYRTHFERVVEPRQVTYTKYVPEIQYREVARTVM
                     KPVWETHTRQVTYTVCKPVWETCTKEIPYTVMKPVWETRTRTYTVCKPVWETCTKEIP
                     YTVWKPVYETVTKEIPYTVMKPVYETRTCTYTVMKPVWETCTKEIPYTVMKPVWETRT
                     RTYTVCKPVWETCTKEIPYT"
     assembly_gap    9411..9510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9511..>10411
                     /locus_tag="PLD05_00955"
     CDS             <9511..>10411
                     /locus_tag="PLD05_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76021.1"
                     /translation="VTKEIPYTVMKPVYETRTCTYTVMKPVWETCTKDVAYTVHRRVY
                     ETRYREVPYTVWKPVYETKMVKVCSGRWETRVVEKPGPVVTKLVHEPGEWKWDATSCR
                     AYYSPGCVKKVQVQCPPVKVCKRVWVPEVKECPVTCVRYVRETCVKKVPYTVCRVVPE
                     KCVRTVTYKVCRWVPEQRTRTYTVCKMVPERRVKTCTYQVCKMVPEQRVKTCVYKVCR
                     WVPEQRTCTYQVRKWIREQRIRTCTYKVCRWVPEQRTRTYTVCKMVPERRVKTCTYQV
                     CKMVPEQRVKTCTYKVRRWVREQR"
     assembly_gap    10412..10511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10512..11440
                     /locus_tag="PLD05_00960"
     CDS             <10512..11440
                     /locus_tag="PLD05_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76022.1"
                     /translation="QRVKTCTYKVRRWVREQRTCTYQVCKMVPEQRVKVCTYKVCRWV
                     PEQRTRTYTVCKMVPERRVKTCTYQVCKMVPEQRVKTCTYKVRRWVREQRTCTYQVCK
                     MVPEQRVKVCTYKVCRWVPEQRTRTYTVCKMVPERRVKTCTYQVCKMVPEQRVKTCTY
                     KVRRWVREQRTCTYQVCKMVPEQRVKVCTYRVCRMVPEQRVKTCTYKVCKLVPTTCVT
                     KVPCRVWKPVVCTKTVCDVRWVKKVVPCTVTRCVPRIVCEMVPVKVCCPVRVEKACAP
                     ACDACGAPSANEKPLDVKPAQAAPKPLNSRTI"
     gene            complement(11629..>12395)
                     /locus_tag="PLD05_00965"
     CDS             complement(11629..>12395)
                     /locus_tag="PLD05_00965"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76023.1"
                     /translation="VMLSTVLTVQTDAHARLRCQMSSVASRGWVSAFNGYDLNGWVVE
                     GTETFKEGDVEKPVWYVEDGKIICAGKGFGFLRYDKSLCDFSLYLEFRMSEKCNSGIG
                     IRASKFDPKKPDTRPSISGFEIQILDDAGKEPDVHSSMSLYRYVAPLKNAIKPAGEWN
                     WVQITARGTHLRVVLNGQVVHDLDQTDYEKIKDKPLCGFFSVQNHGGYIEFRNIFYKD
                     LTPTVAAGVRRVGPLARLIQQLRAWRAARSQRCLCR"
     assembly_gap    12396..12495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12496..15227)
                     /locus_tag="PLD05_00970"
     CDS             complement(<12496..15227)
                     /locus_tag="PLD05_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76024.1"
                     /translation="MKQIRPKGFLGVFFFLIWCLGIWCSAARAADLKGEAYVGRPFGV
                     GKIELVITPDILPAPLGVEGILLKEANGRVHYPVVATSAVGSFFAGLVGAPPRATFYF
                     LFRGDEPLEIELMARAPIRSILRPRSDPRQWNRLLSAWWREYSQPPGLFESKPDYPPL
                     VAQYLRLMLAQRLGLRPPRLSSQSWEVWFREQLGLTLSSESMRVSMMETRFFDPAAVT
                     SPADQDLPDPWDNQPSSLPDIPANVAIEPIASHVPVECLYIRFGSFRNFLWLQDTLAR
                     WGGDIQNLVALRSLNYQMGQKIEQQLVLRQSALSRLFGETVIADVAIIGYDLYFHEGA
                     SFGLLFQAKNNLLFQTNLLQQRRKRLAQADVREEKLTILGHPVSYLFSQDGTVRSYYA
                     THGDFHLITTSRRLVERFLEASQGKRPLSDSQEFRFARSLFPPSRPDTAFIYLSREFF
                     QNLTAPAYRIETQRRMQALTDLDLVELALLAAASEGVNAETIEDLVARGYLPKGFGPR
                     SDGSFPVLEQGLVRDSLRGSRGGFVPIADLPATRASAAEFRMYRDFVAHYAQAWQRLD
                     PMMVAIQRKSEGPTQERIIIDARLTPLAKENYEKLLTRLGPPDRQQLAPIAGDLGRFE
                     IVLSGGRLFGGLYDVVPPIDIVDGRIVPTGRVRDILIGYLGTTGELGWIGKLNRTFST
                     RPDPNGYSRGLLGLWRREMPGVTVFSFQPEVLAAVTEQLHWIEAPEEAHIRVELKDLS
                     HAQITPLLNRFVYWRTQQTSLGNLLFLRDLEQQLHVPGEECKNVAEIIFDAQLQCPLG
                     GEYVFQPDETGIPRWTSTAFLPTVSPTGSGIQLPFLQFGNQPTSSIPGPVDPIAEREK
                     AMGSAPENVARPDQREEPKASQRPAKSSQEFLAPPLNWFRGLSGAAVVKPEAVTA"
     assembly_gap    15343..15442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15443..16209)
                     /locus_tag="PLD05_00975"
     CDS             complement(<15443..16209)
                     /locus_tag="PLD05_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOP76025.1"
                     /translation="MMDYLQILLLAIVQGLAEFLPISSSGHVMVLAHLCDLSGTPLPD
                     KLAVNVILHFGTLLAVLVYYRGRIVAMIRHDWKTIGLLAVASLPAGFIGVGYKLWLED
                     WLTSQMGTNLLEHPLTAGLMFFATGAILVFVRREEGKASSRDMTILQALLIGFCQAAA
                     ILPGLSRSGVTIAAGLKCGLKREEAGTFSFLLAIPAIMGATLIEGKDFFRSPGFEESW
                     GPMLIGLVAAFVTGLVALEWLVGWLKRGRLAWFAPWV"
     gene            complement(17014..17928)
                     /locus_tag="PLD05_00980"
     CDS             complement(17014..17928)
                     /locus_tag="PLD05_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOP76026.1"
                     /translation="MDEPIAFLNGEYIPQSRLGISVVDSGFVLGVTVAEQIRTFRGQV
                     FRAEDHLERLFHSLQIVGVQIDFSPSQLLSVIQHVAQTNHQLLDPRDDLGVSVFVTPG
                     RYIRYSGPGPVVPTVCVHTYPLPFFLWAKKYRTGESAAISTVRQVPTSCWPRELKGRS
                     RMHYYLAEQEVQKRYPGSRAILLDQDDSVTESSIANVVMCDRDGRLLAPPLERTLPGI
                     SLKVVTELASKLGIPFEFCPLTVAYLETATEIFFTSTPYCVLPVTSFNGRPVGNGQPG
                     TVFAKILAAWNELTGVDIVAQAELFSDR"
     gene            complement(18091..19290)
                     /locus_tag="PLD05_00985"
     CDS             complement(18091..19290)
                     /locus_tag="PLD05_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOP76027.1"
                     /translation="MTPWKTLTFGAVMLSQWENLAMKVARNNTLAAIAAVLTIFAGWT
                     VDAGTTAQVREKSLHDVSAEAYPEHTATLPIQEVGSDAYPFRDRTVTLSSLPTPLKPG
                     ENGNTSDRTYRFLVIDTRSIPTGTLKNPPEALTDLRFFAADESGMVRRLTTDDWVEDI
                     EKEQPLWVVVHGNRVDSGETLAFMTAFCRMCRQVGLNGVFVLWSWPSGVVVRGIARDS
                     RLKAVRADLESSVLASWLASHSLQAPVNLVGYSFGARTVMQAAWQIATQKSVALSDLP
                     GSDARSHDSPAFTLFLVAPAMDTATFDRTIRETLEASPSLKVFVTVNKADPALRWYRH
                     LWSCHGPSALGWQGPFYRTQKDLVDSLEVLDVTRQVGHTHRWEAYLSAPTLRRAFQKT
                     GESLSTP"
     gene            19878..20123
                     /locus_tag="PLD05_00990"
     CDS             19878..20123
                     /locus_tag="PLD05_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76028.1"
                     /translation="MRRMTVVAFGLGVMLLTIGCQRCNLFRRGALFAPTTVPTAVIEC
                     PPVDPCTETVVGSACDPCVPATTVVPPTSTFVPGPAG"
     gene            complement(20423..22132)
                     /locus_tag="PLD05_00995"
     CDS             complement(20423..22132)
                     /locus_tag="PLD05_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP76029.1"
                     /translation="MNHNSNDRIDGYGMLISRYLLVMFFGKGLLPVGLALCLFSITQV
                     LADHGQPRGFATSHPGIGSVNAPPFTIFAAGPGHDGFIPVGVATSGQVKVDSAGSDVP
                     FEIGQFQDVRKAGAVGDGQQDDTAAIQACIGRGGAIVFPPGAYRITRPLEVNLAETGW
                     VSFHGFGTARLQMAGAGPAIRFLGTHTGSADPKRVHPGVWQHERFPVVEGIEIVGDHP
                     EASGIEAIQTMQLTIANTLIRQTFHGIRLHQRNRNVILSHCHIYENRGIGVFLDDVDL
                     HQINIVGCHISYNAAGGIVVRKGYLRNLQVSGCDIEANMSVETEPTANILIDSRESEY
                     GHAEIAITGCTIQHYSKVPGSTNVRFFGQDAKGRLWGNLTIAENVLSDVERNIEIIGA
                     RGVCITGNTFWGAVAEDLLITDSRNIVVGPNTFDQNPNYAHQGPYQGGVCLRNCNDCT
                     LNGLQFADVQAPQAALLVQNCHGINIINCQFTDCGPAGILLDQVTDSRVSDCIIRVAA
                     AGSQGPPSGTEVPAVPPADIIAIRGIGLQNVMIRGNLLCGKIDLPDSGVHREANLELP
                     PLP"
     gene            22578..23573
                     /locus_tag="PLD05_01000"
     CDS             22578..23573
                     /locus_tag="PLD05_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOP76030.1"
                     /translation="MQPSRFGAIATRWKTCTLVFLVLSGLCLGCVRNRSALRDGQHVE
                     VAVTIPPQKWLVEKIGGDRVVCHVLVAAKNDPHTYLGTDADAARLARCRLFFTIGLPI
                     EKAPWCQAVLRSRGVSVVSLGEEGEDDHDHDHADHHGGEEQHQDGSDDPHEHEHGRHV
                     WLSPRRLSRQAEKIAEALCRVDPSGAVEYQKNLVKTQEELRELDRELSQKLEPLRGQT
                     FFVFHPAWECFAEDYGLIQQAVQVQGKEPSDHEMTELQRKARQTKLKVILVQPQFSSR
                     AAEAIAEVAGLQVVVADPLTEEIPTVLRQVADLLVSAYRETYGAARTETPSTGQP"
     gene            23586..24488
                     /locus_tag="PLD05_01005"
     CDS             23586..24488
                     /locus_tag="PLD05_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76031.1"
                     /translation="MLSNPSSDQKLGSESAPHPSVAASEEIVVQVEGVSFTYAQQNGA
                     GSATKWVLEDVSLKVFADDFLGIIGPNGGGKTTLLKIILGLLEPQKGWVRVFGRTPRE
                     VRHQIGYVPQHARIDATVTATVLDIVLAGRIARSRWGFRYGKRDREAAFVALEMTGVA
                     PLAHRRIGTLSGGQRQRVLIARALAAEPKLLLLDEPTAGIDPYVEQNLTDLLHQLNES
                     LPIVVVSHDIGFVSTHLKRVACLNRRLTVHRVSEITQATMSDFYREHVHMVRHLASCP
                     LGDAGCQHGCQSSTESVTREGQSD"
     gene            24481..25326
                     /locus_tag="PLD05_01010"
     CDS             24481..25326
                     /locus_tag="PLD05_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOP76032.1"
                     /translation="MIDFFHDWADNPFLWSGLWAGLLAGLACGITGPYVITKRIVFLA
                     GAIAHIAVGGIGASVFLAHKFPQLCGSLQPIQGATAVAIISAIVVGLVNEYWAERSDT
                     IIGALWAIGMAVGLLLVKFTPGYQGELMSYLFGNLAFVSQRQLQWIGFLDVVIILIVL
                     LFHKQFIAVCLDQEQARLQGVPVTFINLLLLSVVALTVICLTSMVGLILVIALLSLPA
                     ATVARFVNGMLAMMVWSTILSCFLTTVPRILVYGTPVNPESAIVIAAAALYLMSLFLA
                     GRTAR"
     gene            complement(25523..26851)
                     /locus_tag="PLD05_01015"
     CDS             complement(25523..26851)
                     /locus_tag="PLD05_01015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOP76033.1"
                     /translation="MWSFARRDPEGAALTRTSPRGVPYLTRPQDGERNKDFDMILQAK
                     KKMAISVLWAVLILCSTPVLLAEDERCLPFVSPAQVGMKAEVLAKIPVRLQEFVEARE
                     IAGAVTLVARNGSIVHWEAVGWADIEKQIPMRKDTLFQIASMTKPITATALMVLVDRG
                     KVALDDPVSRYLPEFKEITVKGQRLTRPLTIKHLLTHTSGLTGSQRTEATLQATVEAI
                     VKGNLAFQPGERWNYSPGLSVCGRIIEVVSGMPYEQFLEKEIFQPLEMVDTTFVPNEH
                     QRERLAKAYQRTADGQLLPGGGASWLIEDAGVRAPNPSGGLFSTAGDLVRFYQMILNG
                     GELCGRRILSPEAVRLMTTVQTDDLQTGFTPGNGWGLGWCIVRQPQGVTEMLSPGTFG
                     HGGAFGTQGWIDPQRQMIFILLVQRTNFPNADASAIREAFQRLAVEALEL"
     gene            complement(27017..27568)
                     /gene="rplI"
                     /locus_tag="PLD05_01020"
     CDS             complement(27017..27568)
                     /gene="rplI"
                     /locus_tag="PLD05_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOP76034.1"
                     /translation="MMATGKGTKKNRKHGLRGLPRGPHGGVQLLLVQNVEHLGKAGDV
                     VEVKRGYAVNYLIPQGLATIATEHHKRMVEKHKAKLLEIERARISTMKQLAENISQMS
                     ISIEANANEEGHLYGSVGADDISRALKAQNININPDQVKLQGPLKELGLYTVQIHLGH
                     DIYADLKVWVVPMVGDTRPGVSR"
     gene            complement(27637..28080)
                     /locus_tag="PLD05_01025"
     CDS             complement(27637..28080)
                     /locus_tag="PLD05_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOP76035.1"
                     /translation="MASFNRVILVGNLTRDPNVRYTPSGTAVCEIGLAVNERRKNQSG
                     EWVDEVVYVDVTLWGRTAEVAGEYLTKGSPLLIEGRLRMDTWEKDGEKRSKLRVVAER
                     MQMLGTRGAQNDSGARPGGTKPPVPGPVEGVLDETGFPPEDDVPF"
     assembly_gap    28243..28342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28531..29100)
                     /gene="pth"
                     /locus_tag="PLD05_01030"
     CDS             complement(28531..29100)
                     /gene="pth"
                     /locus_tag="PLD05_01030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOP76036.1"
                     /translation="MKLVVGLGNPGRQYVGTRHNVGYEVLAELARRWAGEVRPRRQFS
                     GETVDATINGIKVLLLSPTTYMNRSGTSLRAACDFYKLKPQDVLVICDDMNLPLGRLR
                     VRPRGSSGGHKGLADIIRAMGTEEIPRLRVGIGKPPPGEDSVRFVLSVFGKEEEAVIR
                     EAYRKAADAVETWVAEGIDVCMTRFNGGN"
     gene            complement(29205..29840)
                     /locus_tag="PLD05_01035"
     CDS             complement(29205..29840)
                     /locus_tag="PLD05_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOP76037.1"
                     /translation="MAERIVVELRQPGGKRSNRRLRDAGKIPAILYGHKLEPVCLSVP
                     GDALAAAVRHGTRLIQLDGAVKEQAYIKELQWDVWGKEILHVDFTRVSAHERIRSEVA
                     IELRGESPGVKLGGRLAQHLHSIEIECEVDKIPEKIFVNISHLNIGDSITVKDLSVPQ
                     GTKILADPDEIVVVCHPPAGEVEEEAAPEVPGPAEPEVIGRKKAEAEESEE"
     gene            complement(30152..31144)
                     /locus_tag="PLD05_01040"
     CDS             complement(30152..31144)
                     /locus_tag="PLD05_01040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOP76038.1"
                     /translation="MRTSRKVDEGVCAMIHEMKIFSGRANVELAQKVADYVGLPLGRI
                     SLGRFPDGEISVKIEEDVRGRDIFIVQPTCPPVNENLMELLIMIDSFKRASAARITAV
                     IPYFGYARQDRKDEGRVPITAKLVANLITRAGADRVLAMDLHAPQLQGFFDIPVDHLY
                     AAPVLDAYFLSLGIPLEDIVVVSPDEGSIKRTLLHVPVFGGKLAIVDKRRSGNSTSAA
                     HLIGESVKGKVALLFDDMISTGSSISTAAHMVKNAGAAKVYLAATHAVFCGPAVERLA
                     AAPAEEIVVTDTIPLDSKRVLPNLKVLTVSELLGEAVKRIHRHESVSKLFKSYR"
     gene            complement(31220..32443)
                     /locus_tag="PLD05_01045"
     CDS             complement(31220..32443)
                     /locus_tag="PLD05_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP76039.1"
                     /translation="MARRYLNERLARMVLLKSSPSLMKRAHISVLHLAGRLNPGGIET
                     WLLQVLQAAIETHFHMDVLVVTDQPGELDDAIRQAGARLFVTNRPLNPLGQLHDLRAI
                     HRQWGPYDIIHSHVQPGGIQLRLAQHCGISIRIAHSHTTELLAPGLRFHQKIVARLTK
                     SWVRRYATHGLACSERAAETLFGKNWRDDPRWQVLHYGIDLTPFRERSERPGELRHSL
                     GLPEDAFVIGHVGRMSPEKNHKFLLTLAQALVTRMPETYLVLVGDGPVRRQVEKWIEQ
                     KGLTERVRLLGSRRDVPALMMNLFDLFLFPSIYEGLPLVVIEAQAAGLPIVASSAVPE
                     EAIVIPQLIRRLSLDEPLTAWVEAVTAATQSAHAIPRDRCLSLMERSDFNIETCTRKL
                     LQIYQEAVSSTRPLD"
     gene            32779..33642
                     /gene="trxA"
                     /locus_tag="PLD05_01050"
     CDS             32779..33642
                     /gene="trxA"
                     /locus_tag="PLD05_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOP76040.1"
                     /translation="MQSAQPWVISVTTATFQRDVLDQSRNVPVVVDFWAPWCGPCRML
                     GPLLEKIAKEKQGAFILAKVNTDECPDLAAAFGVQGIPAVFAVRDGRIVDHFMGALPE
                     TDLRAWIDGVLPQRDEQLVAQADSLAEQDPHRAEELYRQALAENPKSIPARLGLAKLF
                     AGRRQFEELKTILQELSDMGVSGEEIERLRASVEFAESTSSTDEIAELQRQVASDPSN
                     HALRLQLAKALASRQHYQEALDEALAVVQAAQGELRDDARKLMIQVFSLLGNEHPLTA
                     EYRRRLTMALF"
     gene            33747..>35367
                     /gene="uvrA"
                     /locus_tag="PLD05_01055"
     CDS             33747..>35367
                     /gene="uvrA"
                     /locus_tag="PLD05_01055"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HOP76041.1"
                     /translation="MNTQPQISIPSPSAIRIRGARVHNLKNIDVDIPHERLVVLSGPS
                     GSGKSSLAFDTIFAEAQRAYLETLSPYLRQFCLQLPQPDVDLIEGLPPVVAIAQWAGA
                     SQPRSTVGTITEVYDFLRLLYAKVGVLHCPHCAVPLGSQSPENIATELMRLPEGTRVM
                     ILAPFVFQKRGEHKEVFRHIQKLGFPRARVDGAIVDVEDPPLLDASATHSIEAVIDRV
                     IVRAGIDSRLMESIHLALKLGQGVLIVCHEECDSQGETRWVDRFFSTTRHCAQCGESY
                     PELEPRTFSFNSPYGACARCQGLGEIEQFDPDLVLDWNKGLGNGAVKLWTDPRESLKH
                     HEKKLARLMKMGKFDWHTPLVKIPKEYQIQLIYGQKPSHMQGAMNPTDGETSASRTEA
                     ANSGRSGFLGLLHLLEVEYELLADGRKRRAWASYRKPVTCPDCGGARLGPIGRSVLVA
                     GLPLHQLSAMNVDDLQSYLAQLAFSPLEQMVAEPILREVQSRLGFLKQVGLGYLSLNR
                     PIDTLSGGEFQRVRLASGLGAALTNVCYVLDE"
     assembly_gap    35368..35467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35468..36397
                     /gene="uvrA"
                     /locus_tag="PLD05_01060"
     CDS             <35468..36397
                     /gene="uvrA"
                     /locus_tag="PLD05_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650187.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HOP76042.1"
                     /translation="VSGSGKSSLLAQTLVPALKKEVMKLPASPGPYSRLRGLKHIDRV
                     VEIDQTPIGRSPRSNPATYSGVFDEIRKVFAETREARRLGFGPSRFSFNTPGGRCELC
                     EGLGVRRIEMDFLPDMYTICPRCLGRRFNQQTLEVRYREKTIADVLEMSVSEARQFFE
                     NFPSISRLLSSLDDVGLGYLRLGQPAHTLSGGEAQRIKLGTELGRVQHGRTVYVMDEP
                     TTGLHFQDIQRLLMVIHRLVDAGNTVIVVEHHLDVIKSADWIIDLGPEGGRAGGEIVA
                     VGPPEEIAKVERSHTGRYLKQILNSCASDSSAQ"
     gene            complement(36665..>38905)
                     /locus_tag="PLD05_01065"
     CDS             complement(36665..>38905)
                     /locus_tag="PLD05_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspD"
                     /protein_id="HOP76043.1"
                     /translation="QNRPRIEREQKNQEISEKISQEANDLRDRQTKLAQMVDQYNQLM
                     REQRFAEAEVVAKKAAELDPENPVTKQLVLNARFIRQYSESMAIKDEKEQGFLAQLAS
                     VDRASIPFDDSNPYIMPDATSWADLTKRRSKLTEAGRQRSEQELEIERKLQTPVMLQF
                     ENAALVRVLDYLAKLADINLHIDPQGLASEGVTTDAPVTINLTQQISLKSALNLILEP
                     LHLTYVIKDDVLKITSEQYRRGTVYPVVYNVADLVSPIPNFVGEGLHLDASYQQALSQ
                     LGIGPNASPISPVAMVAGRDGSAATAAVNPMILAQMSSAAAHASPGRSTAPFSTGRGG
                     MGGAALADFDSLIDLIETTVSPNSWDSVGGPGSISPFPTNLSLVISQTQEVHEQISDL
                     LEQLRRMQDLQVTVEVRFITLNDNFFERIGVDFDFDIDDKIDGRHGVRFGQIVQAGDP
                     TGDADEQLDVRDLRDRDNGKGVTVGLSAPNVFSADLDIPFRQGSYSLAVPQFGGYDPT
                     AGASLGFAILSDIEAFFFIEAAQGDRRSNVLQAPKVTLFNGQYGMVADQSTSPFVISV
                     VPVVGDFAAAQQPVVTMVNEGTFLTVQPVVSQDRRFVRMTIIPYFAKIKEVNEFTFVG
                     SETTTETTSAEGNQDQPNDATKKANARSTTRSGTTVQLPTVSSFYVLTTVSVPDGGTV
                     LLGGIKRLSEGRNEFGVPLLSKIPYVNRLFKNVGVGRETQSLMMMVTPRIIIAEEEEE
                     KLGITQ"
     assembly_gap    38906..39005
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39006..40562)
                     /locus_tag="PLD05_01070"
     CDS             complement(<39006..40562)
                     /locus_tag="PLD05_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76044.1"
                     /translation="MNARVRIYQGMVAFCLAFLILNCPALGQGVGQGTDNGPVANADQ
                     LRRQCDDLLARARQAIAEGNLNGAEQLLHQAEQLGVNYPPLYLGDTPAKVRKALESAR
                     QAQPAGQTMPQSPFDARGLGQTPDPKTQAKNYVRNGRMALARGNLSEAAQWCQMAAGL
                     PATFAPEEDSPSKLAEDIRRAGGQVAGATPGAVTSLPPVGQVTQVAPPANASQKSPAQ
                     QQSDQWLLEARRALALGDVRTARRYVEQVRALGLTYGRYDDSPDRVEALIQTHENLLS
                     QKAERGQTEGWRRLYAKLLLEEADGLLRWGDLDEAERLAQLAAQQGVTFSPFEMKPET
                     ILERAANERRNRGLNPALAAQKQRAMQLLAQARSSLAMSDVSTAETLAHQILALNLPE
                     SVFSPNEDSPARLISDIQQAKQAGAVRPASAQAVVPASGPYSGNVAAAAVYDPTRDWT
                     RNVPASTSQPAPTSRGMMLVQQAEDALRRGDYAVAIDYFRQATAYLHELDPATAQRVQ
                     DVLQQDAARTA"
     gene            complement(41277..42404)
                     /locus_tag="PLD05_01075"
     CDS             complement(41277..42404)
                     /locus_tag="PLD05_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76045.1"
                     /translation="MIEPLDVSSLPALPAPMWFIQFFKVLGFILHMIPMNLWLAGMIL
                     AVFFSRWQGSPRRWAVRLGRQMPIIVAFGINFGIVPLLFIQVAYPWAFYPATILTAWF
                     WLAIIALLIIAYYGVYAFAFGLNDEERPIPAWRLLAGWISAGVFIVIAILFSNGLSLM
                     AAPERWKFIWFSNQVAGAATGTGHNFGDASLWHRWPMVFGMALMTTAVWSLVDAVFFD
                     KRGTLEYRRWLAKFSLLIGIVGAVVYASAGTGYVFGTWPKSVFSYMWSDWRLPLTLFT
                     GASPGLVVATLLWIRYRGVSQGPVLLASLAQVIVLSTNAVSRQLVQNIELTPLVSLNQ
                     LATKPDWGPMALFLVSFAIGVGIIAWMLQQVVRAERSQVEQ"
     gene            complement(42401..43804)
                     /locus_tag="PLD05_01080"
     CDS             complement(42401..43804)
                     /locus_tag="PLD05_01080"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOP76046.1"
                     /translation="MHYPWWYVPVLTSPMLIAVIAVLHVLVAHYAVGGGLFFAVETSF
                     AYRTGNREYLDYLRRHAPFFILLTVVFGAITGVGIWWTIGLASPLATEVLIRTFVFGW
                     ATEWVFFVIELTAAFIFYYYWGRLAPRTHQIVAWIYAAAAWISLVLITGITAFMLSPG
                     RWLESPSFWTGFFNPQFLPQVISRTGGAILLTSLYVYLHSSLLLPVGEVRSLIVRRST
                     RPAFVGAILITIGGIGWLLFLPESARLALEAAAVLTVLTTLIFALTVAVFVLLYFGPY
                     RNPGWMTPGFAASLLLFGIAAFSTGEFIREAVRKPYVVYGVVLGNQVLEEEVPELRRN
                     GFLQSGRWTRAYVMEHYPQVVGEEREEKKIMPEKLLALPESDRRHLGQVIFMHHCNDC
                     HAAESGYSAVGPILFSRPRPMLVDMIRHLHETHYFMPPWSGTPEEAELLADYLLTLAP
                     ERPRGIYPSLEKLENQP"
     gene            complement(43983..44216)
                     /locus_tag="PLD05_01085"
     CDS             complement(43983..44216)
                     /locus_tag="PLD05_01085"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOP76047.1"
                     /translation="MTIERGLRLMAGVVVTLSAALAYFHSPLWLILTAFVGLNLIQSA
                     FTNWCPAVWMLSKLGLRACCECEQTNSVASPTG"
     gene            complement(44241..44462)
                     /locus_tag="PLD05_01090"
     CDS             complement(44241..44462)
                     /locus_tag="PLD05_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76048.1"
                     /translation="MSRDLVLIIAIFAGWIILNKWILPALGIPTCMSGGCCGPSACST
                     PFEESSPATQERAPDEGCSVSPPIEDEPK"
     gene            complement(44492..44743)
                     /locus_tag="PLD05_01095"
     CDS             complement(44492..44743)
                     /locus_tag="PLD05_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOP76049.1"
                     /translation="MKCDELLAALNAYIDGEIDPQVCAELSRHLEGCNPCRVVIDTLR
                     KTITLYKGEVPYELPPEVHARLHECLKKKWQERFGQSVS"
     gene            45075..47366
                     /locus_tag="PLD05_01100"
     CDS             45075..47366
                     /locus_tag="PLD05_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOP76050.1"
                     /translation="MAGWILNLPPTGAWYNWRPGVPHRKSNLAVDVFWVILWTVISTL
                     PLQPLFAESGLRFARRVINPESEFTACAIFDVNGDGRLDIVAGGWWYEAPTWRKHFLR
                     DVPQIRGRYDDYSNLSLDVNGDGWPDVISANYRSESLFWIQHPGPGLGPWQVHLIEKP
                     GPMETGRLYDVDANGTLDVLPNGMKFAAWWEFRREDTGNVRWIRHDLPREIAGHGIGF
                     GDVNGDGRGDVVTPIGWWEAPKDRANGEWIAHREWVLHRDASVPIIVEDIDGDGDSDI
                     MWGRGHDVGLYWLEQARDGDSRRWTFHVIDTSFSQIHSILVTDLDNDGRREFVAGKRY
                     LGHEGRDPGEWNPLAIYWYRFDPGRRTFIRGVVDEGGPVGFGLDPKAADIDGDGDIDL
                     VASDRSGLYLLENLLKSDPEQTSDVLPPSSEEGPWTKEDHRNVLLYVDENNETKTVSS
                     LKEWGIRRAHILKGMMAAMGPLPGPEHRVPLDIEIVSEEMTESYRRLKLTYAADESDR
                     VPAYLLIPTRPSQRKPAVLCLHQTTPLAKDEPAGTHPDAAWPYAHELALRGFVCIVPD
                     YPSFGEHAYDFRAHPEYASGTMKAIWDNIRAVDLLESLPEVDRDRIAVIGHSLGGHNA
                     LFTAAFEQRIRAVICSCGFTGFQDYYKGKIAPWAQDRYMPRISTVYGNDPEKVPFDFY
                     EVLAALAPRPVFVNAPLRDANFDCDGVKKILIAIEPVYALHNVRDRIKAVHPDCEHSF
                     PAEIRQAAYQWLEEQFGLAKSTN"
     gene            47730..49583
                     /locus_tag="PLD05_01105"
     CDS             47730..49583
                     /locus_tag="PLD05_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76051.1"
                     /translation="MGQAEEQYLGPWQVRATADGSRVLVLCKDAAKIAIIDPNTQQIV
                     GWIATPAQPSDFVISPDGQTLFVSAGVADGIVVVLDNSGKRLKEFRVGHSPCGLSLSP
                     DGRFLFVCNRFKNDVTVIDLLKGQTIACPKVIREPVCSAITPDGTKLFVANLLPYDRC
                     DSYDVAASVTCINTQSFETTHIRLLNGSTDVHGLTVSPDGRWVFVVHVLARYQMPTTQ
                     LERGWMNTNALTIIDAAALKRVNTVLLDDVDLGAALPWSVVCSRDGSTVLVSHASTDE
                     VTVVNVPGVLEKLASLPATQEEAKGLGRIYDGSQTSLSAVDVPNDLSFLVGLKERMPL
                     YGTGPYDYLASQNEKIIKGARGLAAVGNKVFVATYFGDCVTVIDLSASKYRRLSYIAL
                     GPTPKLTQERLGEMHFHDASLCFQHWQSCATCHPDGRIDGLNWDLLNDGIGTPKNNKS
                     LLFAHVTPPSMWEGVRATAEDAVRSGIRHIQFAVRPEEDAQAIDAYLKALKPVPSPYL
                     VNGELSESAKRGRELFFSDRLGCRHCHPEPLYTDLKMHNVKSRGKLDRRDEFDTPTLI
                     EIWRTAPYLHDGRYLTLEELFREGQHGQTVGDVAGLSDTELHDLCEFLLSL"
     gene            complement(49735..50532)
                     /locus_tag="PLD05_01110"
     CDS             complement(49735..50532)
                     /locus_tag="PLD05_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76052.1"
                     /translation="MSTFDPADVLFHVPTLSDAERLVIAGLFPDVLLLLEDHPRQFSE
                     QDWLSFMAHSPLTRAVAIYTDWCESEPRTGSPLPGVIRISWLRWWNFGRALLHALRQG
                     SGSPWNLPPTASEEERLLVSLKYTPLVKTDSALKHIAIAAPTQDSFTWLRQAVLPFTS
                     CVSWVHRAIGRNQPGTPPDLLIIDIGTDGCTIEDSLNVLRANLWPDLSPAVPCILLMN
                     FPRWQQVWPLLARGTTRVISKPVFLPELEWVLAELAQTRDVTCRSPR"
     gene            50973..51209
                     /gene="csrA"
                     /locus_tag="PLD05_01115"
     CDS             50973..51209
                     /gene="csrA"
                     /locus_tag="PLD05_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOP76053.1"
                     /translation="MLVLSRQRDESIIIGDNIVVTVVDIRGDKVRLGIQAPPEITVHR
                     REVYEAIQREKKMKESQQANPSVQPEVSGQKETT"
     gene            51628..>52538
                     /locus_tag="PLD05_01120"
     CDS             51628..>52538
                     /locus_tag="PLD05_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOP76054.1"
                     /translation="MTRINTNITSLNAQKTLARSQAQLQEALTRLSTGLRINVGKDDP
                     AGLIASEALRADITAVQKAISNSERANQMIATADSALGQISQLLNDIRGLVSEAANKG
                     AMSAEQIAANQLQIDSSLEAIDRIAKVTQFQGQRLLDGSFDFITTGVDTSKITSLRID
                     QANFGALSSISVNVDIVSQASKAKLTYNYGALATDVVLEIAGKDGAEAFSFAAGSSIE
                     DMAQAINLVSDALGVRAVVHQEATAGSLTASSFGSNNDVIITANEAGQAAGDLRIKYT
                     KSSSATTSVVYTPASGNTPGLIEVQLGT"
BASE COUNT        11680 a        13978 c        14727 g        11353 t
ORIGIN      
        1 ccgggagagg gtcggggtga gggcaagcga acgtcggaat cgattgagaa aaccggcagt
       61 aattcgagcc acggcgcgtc tctcgccgaa cgggtgagaa gagattatgg gataggttct
      121 aaacgttggt tggaggttgg gctggcgggg ttgactcagg acgcggcggc aacgccccaa
      181 atgaggcgga cggcccgggt ggtggggtat cctcccccgt ttcagagacc aggacaattc
      241 gatgggagcg accggcatta cccggcgcgg aatgggttgc ggggcgagat gacgcaggtt
      301 gagatgttgc ctgtccgttg ttctgtctca ttgcctccca ttgttcaaga gacatgagga
      361 cctctcgagc ttgtgagccg ttgtacggcc caacaatgcc gtcttcctcc atgtagtcga
      421 tcaagcgggc agccctgccg taaccaattc ccagggcccg ttgcaacaaa gagacactcc
      481 cgcggccttc ccggataaca atgtccacgg ccgcctcata aagttcatca cgggcggcca
      541 gtcccggttt gtttggatca ccgggttggg aaggcttcag ttggacaagc tcctggacaa
      601 attccggctc gtgtacggca acggcatcaa tgacgcgatt gatttcgtcg tcactgacgt
      661 aggtaccttg gccgcgaatc agtgtgcttg tccccggacg taggaagagc atatcgccat
      721 tgccgaggag tttttcggca cccatttcgt ccagcacgac gcggctatcc acgcggctgg
      781 ctacctcgaa gcagatacgg gcaggcaggt tggacttaat caggcccgtg atcacatcaa
      841 cggtaggttt ttgcgtagcc aaaaccaggt gaatccctac cgcccggctc ttttgggcaa
      901 ggcggatgat atgggtttcg acctccttgg ctgccgtcat catgaggtcg gccatctcgt
      961 cggcaatgat cacgatatac ggcatatggg tcgggatttg tgcgcgttct tcctcgcttt
     1021 cgggccgaat cctctccatc aactcctctt cgccaagttg gttatannnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnacgt ctttccccag gaagatgggg atccgcatgg
     1201 cctgatattt tccgtgactt tcctcgatga cctcccgcag acgtacaatt tgacgttcgc
     1261 tgttgggcac ctcgatcccc acggtattct ttcccgggat gggggctacg atgcgaacac
     1321 ttggcacgcg aagcgcgatg gccaggtcgt cggccaaggc cgtgaccttg ctcagccgca
     1381 gcccggcttc gagttcgacc tcaaattgtg caatgacggg accggtctgg atttctacga
     1441 cgcgaacgtg gaggccaaaa tcggcgaaag ttttctcaag aatctgggcc ttgcggcgaa
     1501 cttctttctc ttgctcttca aaatggaatg gttccggttc aagaagtagg tccaaaggcg
     1561 gcaatttgta gttgaagtgg cgtcgttcgt cggttgacga atctatgaca tcgagaagct
     1621 cttcttttgg taggcgtgtg ggcttgcgga ttcgaaggtc acccgacttg cgatcattcg
     1681 cttccggttt ttggacggtt tctgcatgtt ggggctggtg ctctttcgcc ggggcaggtg
     1741 tcttgatcag cggcccgcgg tgagaagtat cggtttcacc cggttcgctt ccttgaggtg
     1801 ccgcgtcttt cgcctggcca gacggcactt cggctaattc gccgggccgc ttaacccgca
     1861 ccgcggaatc ttgtgcctta gcctcagatg tgggacgtcc aagcccgtat ccgctggcca
     1921 cttcgcccag tcctttcacg attcccttaa gaggccacaa cactgccgtg agaacagttc
     1981 gaaccagcga cagttcggtg caaaggagaa atcctatcga aaacccggcg aacagcgtgg
     2041 cgaccgtccc ggcaaatccc agtttttgtt ccaggatcca gcgaagggca accccgacgt
     2101 agccgcccgg gccgacaacg ggtccgggac tccagttagg gataacacag tcggcaagtc
     2161 ctgtcagccc aaaaatcgag agaaaccaac caaacacgcg cgtattcggc cgcgtcagcg
     2221 gttgccgcaa cagcagaaca atcgtcaaaa ctgacaacga cagcacggcg taccaagccc
     2281 cgatcccgag gaattccagg gcgtaaaacg cgacggttgc ccccacaggg ccgcagacgt
     2341 tcgtgacctg cgcgtgcggt gggaaaacca agtcgctggg ggaatcggcg gggtcgaatg
     2401 tgatcaagct ggcgccaaag aataagacga ctcccaaaag caggagtgcc acaagatcac
     2461 ggcccaattc cgctcgaagt gatgccatca cgattggtcg cttggacaaa aacgtacgct
     2521 tagccccgtc gcaactcgac gcctgctaca tcctgtttcg aaccagccgc gtacgagccc
     2581 acactcattt ccactcaact cgccgggcaa gtattttcgt cacatctcac atatttcgca
     2641 aagccacttg aggccgtgag agcgttgcca tcagcgctgt ttgctcttgt gttccacttt
     2701 tcacaaagtc acaaatggcc cataaaaaaa ccaccgccgg ggcggtggtt ggagtccagc
     2761 atgcttacgt gggttacttg tgctataagc aatcgttact tcttatttgc ggccatgtgc
     2821 aacgccacaa ggaacggcac gggttcgctg ccgagggcgt tcaacacggt tccgcctgcc
     2881 gtgaagttgt gggtgaccca gtccggctgg ccgtattttt ccaaggctgt gccgccttca
     2941 ccgccgccga cgtacacccg tacaccggca tccttcatcc gcttcaattg agccatgaat
     3001 cgccgggtcc cattctcgaa tttcgggtcc tcaaatttcc cgaaaacacc gttgtggaag
     3061 gccacggcgg gttccttgag cgtttttgcc caatccagga actcgccaat cttccgctcg
     3121 aacagctctg aggttgcagg gccgacatcg aattgctggt caccgggcga aagcgttgtc
     3181 gcgacacggc catcctgcag gacaaaatcc accggcatca caaattggat gccctgggcc
     3241 ctgccactgg cgatcatctt cttggcctgt tcaatccgct ctggcggaat gtaatatggc
     3301 tgatctttgt gggcgggatc ctcggccaca ccgatgcaga agtccttacc gtcaagctga
     3361 taggcagcct tgatcaaacc catcgccagc gagccagcag tgaacaccca gcgaatcttg
     3421 ccccggctaa tcatggcctc gaggtcgtcc agcttgtcga tctttagacc gctcgaaatc
     3481 acaagctggg cgttgaggca gtcggccaag gggcctgaga attcaccgta ggcatattcg
     3541 cccaaggcga ctttttccat ggccgcagtg acaaccaccg aggaagtgtc caaacttccg
     3601 gcggaaaaag cttcatgcac ataaaccttt gcgatctttt ccgcgaattc gttggcaaca
     3661 ttggtaagtt tctcgacgag gttcgggatg tcagcgggtt tggccttcca caagacacgc
     3721 tcgatgtcat acttgcgggt gttttccaac aagatgatgt cgcccggctt ggcagcctta
     3781 atggcgtcaa cgacctcttg tcggatctta agggtttcgg gggcaaacca gtcgcggata
     3841 aagacgattt cgcactccaa tatctcgctc aaccgcttcc ataccttctc taggctcagt
     3901 tgtggatcgc ggccgatgtg tccaaagatc acttgaatcc acccccgatc gcgtccgagg
     3961 aggagggttt ctttcatgga gcggagccga atgtcacctt ctccaacctt tgccccgggc
     4021 ttgcaatcga ggtcaccgcg aacgagcaca ggcgtccccg ctggaacatc cgtgaggtcc
     4081 ttgacccggg gaatgccagc gatgtagtcg agaaggccca tggtgacctt gtccacacca
     4141 agaatccgtc gcaaccaggc ttccatcttc tcttttgtca gcgccatgct tcactctccc
     4201 gatagcagga tgaacctcga aacaccgacc aatcagacac gcaaacccgt tttatcagag
     4261 ttgattgtgg cggaaaagga tacaccgtca ccgtgttgtc tcgccaaacc ccgccctcgt
     4321 acctcgtaca atgcatccaa accgcgggat catcccctct tagtaactac caataaggtt
     4381 accagagtag gttgctttcg ttaaggggcg ccgagggaag ttatgccgga aaactcgcat
     4441 tgacgggaat tgccatggtt tgataaactc gccctcaacc ggagggaagt tttcagaaat
     4501 gacccttgat ttactgtagg cttggggcca ggagggaccg tcatgagtgg aaaggattcc
     4561 cgcgtcaaac gtatcttttc aaggcgcagc cgatgggctg ttctttttgt ggccgttgcc
     4621 gttgttgggg cgacggtggc tgtgcgctca ctctggggac ccaatgcagc acgggctggt
     4681 gggccgggca ttctcggcgc gatcacgggt tctcgccagg cgccaggacc tacggcaact
     4741 gccgcgcgaa cccagcccgg gcccacgacc gcaactccct ccggtgctgc ggcgtcttcg
     4801 gcccgcgatg aacgccctca ggtagtcgcc gttgttaatc gcgagccgat cacccgagac
     4861 gagctgcgaa aggaatgcct ccggcactat ggggaagaag tcctggaaag catcgtcaat
     4921 aaatacctta tcatggaaga atgccagcgc cgcgggatca ccgtcacagc cgaagaagtt
     4981 gatgccgaaa ttgtgcggat ggccacccgc ttcggcatgc ccgtcgagac gtggttcaag
     5041 ttgctaaaag aagaacgcgg gattaagccg gatcaataca agcgggatat catttggccg
     5101 atgctcgccc tgagaaaatt agcaggcgat cggttactca tcagccagga agaactgagg
     5161 cgcgaatttg agagccgcta cgggcccgcg gtcaaggctc gaatgatcgt tgtgagcaag
     5221 aaagagacgg ccgaagccat tcgtgcggaa gcggtggcta accctgatca gtttggtgag
     5281 ctggccaaga aatactccga ggatcccgcc agtgccagcc tcaaagggtt ggtccagccg
     5341 attcgcaagc acgtgggaca aaaggagatc gaggaagttg catttgccct caagccaggc
     5401 gaaatctcgc aggtgatccc cattgcgaat cagttcgtca ttttgaagtg cgaagaacat
     5461 cttccgcctg ccaaagttcg atttgaagac gtcaagggag aaatcgagga actagcgaaa
     5521 gacagcaaga tgagggctgt ggcccacgaa atcttccgcg acttgcaatc gcgggctgaa
     5581 atcgtgaacg tcatgaacga cccacaaaaa cgggctcaaa tgccggatgt cgcggcactt
     5641 atcaataatc ggccggtccg acttgaggac ctgggcgaaa tgtgcatcga tcgtcacggt
     5701 gaggaggtcc tagaaaatct catcagccgc aagctaatcg aacaggcgtg tcgaaagcgc
     5761 aacatccagg tcacggaagc agacgtagac gcggagatcg agagggcggc gagagagaac
     5821 ctgcctttgt tgccggatgg acgtgccgat gtgcagcgat ggatgcaact tgtcacacag
     5881 caacagggga ttaagccaga agtttaccgc aattcgattg tctggcccgc cgtggcactc
     5941 aaaaaacttg tgggggataa cgtctcggtg agccaggagg acctgcaaaa ggcctttgaa
     6001 gcgaactacg gacctcgcgt gcggtgtcgg gcgattgtgc taaataacct gcgacgggca
     6061 cagcaggtgt gggacaaggc tcggtccaat cccacggagg agtacttcgc cgacttggcc
     6121 gaggagtact cgatcgatcc ggccacacgg gccctacgag gtgaaatagc gcccatccaa
     6181 aagcacggcg gccagcccct tctggaacag gaggccttct ccctcaagcc gggtgagatt
     6241 tctggcatca ttcaagtcgc accggagaca tacgtcattc tatactgcct tggacagacc
     6301 aaacccgtgg atgtagactt ggcctcggtg caagagattc ttaaagaaga tttgaaagcg
     6361 aaaaaagtgc ggctggcgat ggcggagcta tacgaaaaga ttcgcagcga ggccacgatt
     6421 gacaattatt tggctggaac aacgcaatct cccaacttaa accgggccgc gcaacgagca
     6481 tcaagcggtg acgttcggta acccaaagtt agtgccgggc gcggaaaaga cgagcctcct
     6541 ttaggccttc cgccgacacc acaattattt gccggcattg ttttgatcct cgggtttgac
     6601 gtaaaactgg ctcaagtcga taccaccccg atgttcttct tctaatagct tgacccgtcg
     6661 ttccaactgt tctaggcggc gaagcatggc gtaatagttt tgtattaccg actcttcttt
     6721 ccgcggacgt gggacggagg gataccccaa gtgacgggac agggccgaaa ataggtaaag
     6781 tgggtccttg cggcgatttg cgagggcaat tttcccctcc ttttccccga aaaggacagg
     6841 ctttcccgtt tcgcctttga gcatttggta gtactggctc cgtacgaaga tcatgtcggc
     6901 aatatcgacc aggccaacct gccggcggag gctgagaatc cagtctttcc agttttcgtc
     6961 gtagattgga tcgcgcgcca tggcctccag accggcatca tagccgagcc ggttgcggca
     7021 cagcgtctcg aactggtaaa gaaacacccc ctggggatcg gcacctttgg cgcggtactc
     7081 ggcctctttt tccagaattt caagggcgat acggaaatcg aggcggccat cctcccgttc
     7141 ggcctcccgg atgacatacg tttggaaggc tgtgaaataa tggcctagcc tggccattgc
     7201 cgtcgaaaaa cccccggtga accgcaactc ggtgaccata aagtccaagg ccagagggag
     7261 tttggttgtg gagagaatct cctgagcaat cgttttaatc aactcttgag ttgaaacccc
     7321 ttgatcaagc cgctcccgga gggcccgaaa gaaataggcc tgttcgatgt attcttcgcg
     7381 ctcaagaact ctgggccgat cgggttcctc tgacatcgca actctttccc gccgactggg
     7441 gaggttcgaa cgacagtagt caagaacaaa cacgcgcttg ggcttactga atgattacca
     7501 ttctaagcct gggcggtttc aaggaacatg cccctggtga gcgccacgcg gcgtgaatgc
     7561 tcggagccct ttaatgctgc ggggctgcgg agcgcccaaa agaaaagacc accgggaagg
     7621 ctggtggttt gagatataac gtgacgtcgc tgagtttatt ggggctggtg agcttcaaag
     7681 agccctactg atagtattct gggttattag gccaaatggc gcctgtagat tggtcgtact
     7741 gccacccgta atccccagag acaaccccgg tcggtttttg acccggggta ctgaccggag
     7801 ccaccttgtt actgttgttg taggggttgg gaggtaaccc gttggcgagg tatggcccgt
     7861 atggatagtt cgggccgggc gtgccgatct cgccagcgag attggttgcg ttataaagct
     7921 gagggaggtc attgttgctg atcgtaggat actggccgtg ctggatgcga tataactcaa
     7981 tttgcgatcg tagcgtgtgc atattgaatt ccagggcact gcgctttgca tctgacgtgg
     8041 atgcggcgaa ctgtgggatg atcacggcgg cgaggatggc catgatcacc acaacgatca
     8101 gaacttcaat cagcgtgaac gcccgacgaa cacctcgatt cataaattgg tctcctctgt
     8161 ctgaaattgt gtcatgtagt taatggttcg tttcttcctg gtctgctcgc ccggggcaga
     8221 gaacttttct actgcaatgt aggttgcgaa caagctagtg gcaaatcttt tcgaggagtt
     8281 tacggcaacc gaacatcttt cattgcgaga ttcggggcat ttagcgggat gagttgcccg
     8341 taagggtctg gttggggcgg aaagtccggc tgtgacttcg cgctaatcgt caaagatgcc
     8401 cgcaagtttt gtattttccc tagtttatat cgtcgtttct tgtggcccca tattttgctg
     8461 gttaacttat ctctccaaat tgccgatatt ttcagttggg taagcacggg agtaattttg
     8521 ggaagcggta gcggttgtgc ggccggtatg agttgagtgg gcaatgtgaa ttatgcacca
     8581 gccgccgatc agctaacgct gccgtttgaa tttggacgaa acctcacaat aggtggaaac
     8641 tgccgcgtcc ccaccaggcc catttttgat ttgctacatc aaaaggaggg ataacatgag
     8701 aaccgtctgg accgtgccgc ttatggcgat gctgatgatc gccgttgcgg cggggaaagt
     8761 cgacgccggt tactgtgggg ctgtgcgact tgccctgcaa ccgactggct gcgcgctgga
     8821 gccgggttgt gggccgcaac agtgctacac ggttctgaaa cctagccgca aacttgtatt
     8881 cgaaccacag caggttacta gctacaggac gcatttcgag cgtgtggtcg agccccgcca
     8941 agtgacatac accaagtatg tcccggaaat ccaataccgg gaggttgccc ggaccgtcat
     9001 gaaaccggtt tgggaaaccc atacccgaca ggtaacttac acggtgtgca agccggtttg
     9061 ggaaacctgc acgaaggaga ttccttacac ggtgatgaag ccggtatggg agacgcggac
     9121 acggacatac acggtgtgca agcctgtgtg ggagacgtgc acgaaggaga ttccgtacac
     9181 ggtatggaag cctgtgtacg agacggtgac gaaggagatt ccgtacacgg tgatgaagcc
     9241 ggtgtacgag acgcggacgt gcacctacac ggtgatgaag cctgtgtggg agacctgcac
     9301 gaaggagatt ccttacacgg tgatgaagcc ggtgtgggag acgcggacgc ggacgtacac
     9361 ggtgtgcaag cctgtgtggg agacgtgcac gaaggagatt ccgtacacgg nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtgacgaa ggagattccg tacacggtga
     9541 tgaagccggt gtacgagacg cggacgtgca cctacacggt gatgaagccg gtatgggaga
     9601 cgtgcacgaa ggatgtggct tataccgtgc accggcgcgt ctatgagact cgttatcgcg
     9661 aagtccccta tactgtgtgg aaacccgtgt atgagaccaa aatggtcaag gtgtgcagcg
     9721 gtcggtggga aacccgcgtg gttgagaaac cgggccctgt tgttaccaag cttgttcatg
     9781 agcccggtga atggaaatgg gacgccacaa gctgccgggc ttattactca cctggctgcg
     9841 tcaagaaggt tcaagtccag tgcccaccgg taaaagtctg caagcgggtt tgggtaccgg
     9901 aggtcaaaga atgccccgtc acgtgtgtcc ggtatgtccg tgaaacctgc gtgaaaaagg
     9961 ttccttacac cgtctgccgc gtcgtgcctg agaagtgtgt caggacggtg acttacaagg
    10021 tttgccgctg ggtaccggag cagcggacac ggacttacac ggtctgcaag atggttccgg
    10081 aacgtcgcgt gaagacctgc acgtatcagg tgtgcaagat ggttccggaa caacgggtca
    10141 agacctgtgt ctataaggtc tgccgctggg taccggagca gcggacctgc acgtatcagg
    10201 ttcggaaatg gatacgagaa caacgaatca ggacctgcac ttacaaggtc tgccggtggg
    10261 tccccgagca acgaacccgg acctacacgg tctgcaagat ggttccggaa cgtcgcgtga
    10321 agacctgcac gtatcaggtg tgcaagatgg ttccggagca gcgggtcaag acttgcacat
    10381 acaaggtgcg gcgttgggtt cgcgagcagc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nagcagcggg tcaagacttg cacatacaag gtgcggcgtt gggttcgcga
    10561 gcagcgaacc tgcacgtatc aggtgtgcaa gatggttccg gaacagcggg tcaaggtttg
    10621 cacctacaaa gtatgccggt gggtgccgga gcaacggacg cggacctaca cggtctgcaa
    10681 aatggttccg gaacgtcgcg tgaagacctg cacgtatcag gtgtgcaaga tggttccgga
    10741 gcagcgggtc aagacttgca catacaaggt gcggcgttgg gttcgcgagc agcggacctg
    10801 cacttaccag gtgtgcaaga tggttccgga acagcgggtc aaggtttgca catacaaggt
    10861 ttgccgctgg gtgccggagc agcggacgcg gacatacacg gtctgcaaga tggttccgga
    10921 gcgtcgcgtg aagacttgca cgtatcaggt ctgcaagatg gttccggaac aacgggtcaa
    10981 gacttgtaca tacaaggtac ggcgttgggt tcgtgagcag cgaacctgca cgtatcaggt
    11041 gtgcaagatg gttccggaac agcgggtcaa ggtttgcacc tacagagtgt gccgtatggt
    11101 tccggagcag cgggtcaaga cctgcaccta caaggtctgc aaactggttc cgaccacgtg
    11161 tgtgaccaaa gttccgtgcc gtgtgtggaa acctgtcgtg tgcaccaaga cggtgtgcga
    11221 cgtccgctgg gtcaagaagg tcgtgccttg cacggtgacc cgttgtgtcc cgcggattgt
    11281 ttgtgagatg gtcccggtga aggtgtgctg cccggttcgg gtcgaaaagg cctgtgcccc
    11341 ggcctgcgac gcctgcggtg ccccaagcgc gaacgagaaa ccgctggatg tcaagcccgc
    11401 ccaggctgcc ccaaaaccac ttaatagccg aaccatctga gtcttgcaaa tgctagcaag
    11461 atcataggcc tggtcctaac ggaaatggcc cggtgacgtc gatcaccggg ccgtactctt
    11521 ttcagtaaag cttacgtctt cgcggtcgcc gaccggtagc ttcaccacgc cctatccaaa
    11581 cagttgggtg ccgcgttttt gtggtttgtc tgataaccgg tcaaggatct agcggcagag
    11641 acatcgttga ctgcgggcgg cacgccaagc acgcaactgt tggatgaggc gggcaagggg
    11701 tcccacgcgg cgcacgcctg ctgccacggt tggcgtcaag tctttataaa aaatgtttcg
    11761 aaattcgata tagcccccgt ggttttgcac agagaagaat ccacacagcg gcttatcctt
    11821 gatcttttcg tagtccgttt gatcgaggtc gtgaacgacc tggccgttaa gcacgacacg
    11881 gaggtgtgtc cctcgtgctg taatctggac ccaattccat tcaccagcgg gcttaatggc
    11941 gtttttcaaa ggagccacgt aacggtacaa agacatactc gagtggacgt ccggttcttt
    12001 tccagcgtca tcaagaattt ggatctcaaa gcccgagatg cttggccgcg tatcgggctt
    12061 cttgggatcg aatttacttg cccgaatacc gatcccactg ttgcatttct ctgacatgcg
    12121 gaattccaaa taaagggaaa agtcgcacag agacttatcg taccgaagga aaccaaaccc
    12181 cttgcctgcg cagataatct tgccgtcctc gacataccat accggttttt caacgtcacc
    12241 ttccttaaat gtctctgtgc cttcgacgac ccaaccgttt aggtcgtagc cgttgaaggc
    12301 actgacccat cccctcgagg caaccgacga catttgacat cgcagacggg catgtgcatc
    12361 cgtctgaacc gttaaaacgg ttgacagcat cacgcnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnngccgt gactgcttct ggcttgacca cggcagcgcc agaaaggcca
    12541 cgaaaccagt tcaaaggtgg agccaggaac tcctgcgacg acttggcggg cctctgtgag
    12601 gcctttggtt cttctctttg atcggggcgg gcgacatttt ccggggcgct ccccatggcc
    12661 ttctcacgct cggcgatggg atctaccggt cccggaatag aggatgttgg ttggttgccg
    12721 aactgcaaaa acggtaattg aataccgctg cctgtgggtg atacagttgg aagaaacgcc
    12781 gtagaagtcc accgcgggat accggtctca tctggttgga aaacgtattc tccccccaaa
    12841 gggcattgta actgtgcgtc aaaaatgatt tctgcgacat tcttacactc ttcaccgggg
    12901 acatggagtt gctgttccaa gtcccgcaaa aagagaaggt tgcccagcga tgtctgttgg
    12961 gttcgccagt aaacaaagcg attgagcaag ggtgttatct gggcatgcga caagtccttt
    13021 agctccacgc ggatatgggc ctcctcgggc gcctcgatcc aatggagttg ctcggtcacc
    13081 gcagccaaga cctccggctg gaaggaaaaa accgtcacgc cgggcatctc acggcgccat
    13141 agtcccaaaa gaccgcgact gtacccgttt gggtcggggc gtgtgctaaa cgtgcggttc
    13201 agtttgccga tccaacctag ctcgccggtt gtgccgagat agccgatcaa aatgtcacgc
    13261 acacgtcctg tggggacaat gcggccatcc acgatgtcga tgggcgggac gacatcgtac
    13321 aaccctccga acaaccgtcc gccactgagg acaatctcaa agcgtcccaa atcccccgcg
    13381 ataggcgcca gttgctgtct gtcgggcgga ccgagccggg taagaagttt ctcgtagttc
    13441 tccttggcaa gcggggtgag tcgcgcgtcg ataatgatcc gttcttgggt ggggccctcg
    13501 ctttttcgct gaatggccac catcataggg tcgaggcgtt gccacgcttg agcatagtgg
    13561 gctacgaaat cccgatacat gcgaaattcg gccgcgcttg cccgggtggc tggtagatcg
    13621 gcgatgggga cgaacccccc acgggaaccc cgcagggaat cacgaacgag accctgttcc
    13681 agcaccggaa atgacccatc gctgcgtgga ccgaagcctt ttggtaagta ccctcgggca
    13741 accagatctt cgatcgtttc ggcattgacg ccttccgacg ccgccgccaa gagtgccaat
    13801 tcgacaaggt ccagatcggt cagggcttgc atccgccgtt gcgtttcgat tcgataggcc
    13861 ggtgccgtga ggttttggaa aaactcccgg gaaaggtaga taaacgccgt atcgggccga
    13921 cttgggggaa ataaagatcg ggcgaatcga aactcctggg agtcactcag gggtcttttt
    13981 ccctggctgg cttcgagaaa gcgttccacc agccgccgcg acgtggtgat caaatggaaa
    14041 tcgccatgcg ttgcatagta cgagcggacc gtaccatctt gggaaaacag gtacgagacc
    14101 gggtgaccca gaattgtgag cttttcctca cgaacatccg cctgggcaag tcgctttctt
    14161 cgctgctgaa gcaagttcgt ttgaaagagg agattgtttt tggcctggaa aagcaagcca
    14221 aacgaggccc cctcgtgaaa gtaaaggtca tacccaatga tggcaacgtc ggcgatgacc
    14281 gtttcgccga aaaggcggga caaggccgac tgacgcagta caagttgctg ttcgatcttc
    14341 tggcccattt gataattcaa tgagcgcagg gccaccaggt tttggatatc accaccccaa
    14401 cgagcgagtg tgtcctggag ccacaggaaa ttgcgaaaac tgccgaaccg tatataaaga
    14461 cactcgacgg ggacgtggga ggcgataggc tcgattgcca cgttggccgg gatatcgggg
    14521 agcgatgagg gctgattatc ccagggatca ggcaggtctt gatccgcagg acttgtcacc
    14581 gctgccgggt cgaagaagcg ggtctccatc atgctcactc gcatcgattc gctgctcagc
    14641 gtcaggccca attgctctcg gaaccacacc tcccagctct gggaacttag acgtggtggg
    14701 cgaagcccaa gccgttgagc cagcattagc cgaaggtact gtgccaccag cgggggatag
    14761 tcaggcttgg actcgaaaag ccctggcggc tggctgtact cgcgccacca agcggacaaa
    14821 agccggttcc actgtcgcgg gtccgaccga ggccgcagga tcgagcgaat cggtgcccgg
    14881 gccattagct cgatttccaa cggttcatct ccccgaaaaa ggaaatagaa tgtggcgcgt
    14941 ggcggagcgc ccaccaatcc ggcgaaaaag ctacccacgg ccgacgtcgc caccacaggg
    15001 taatggaccc gaccgttggc ttctttgagc aaaataccct caacacccag aggagccggc
    15061 aatatatcgg gggttattac aagttcgatc ttacccacgc caaacggcct tccaacgtag
    15121 gcctcccctt tgagatcagc agcccttgcc gccgaacacc agattcccaa acaccaaatg
    15181 agaaagaaga agacgccgag aaatcccttg ggccttattt gtttcacttt gttcctccgt
    15241 ggctgaaatc agtttggtag gcggaattga ccgtgtcccg gtgcgattcc cgcaatccct
    15301 cattctgccg cacgagggac tgagagacaa cagcggataa gcnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnaagaccca cggagcgaac caggctaggc gtccccgttt
    15481 gagccaaccg actaaccatt ccagggctac aagccccgtt acgaaagccg ccacaaggcc
    15541 gatcagcata ggcccccaag actcctcgaa accgggcgag cggaagaagt cttttccttc
    15601 aataagtgtc gcacccatga tggcaggaat cgcaagaaga aaagagaagg ttccagcctc
    15661 ttcccgtttt aggccgcact tgagtccggc ggcgatcgtc acaccgcttc gcgagagccc
    15721 gggaagaata gcggcggcct ggcagaatcc aatcaatagt gcttgcagaa ttgtcatatc
    15781 ccgtgacgat gccttgccct cttcacggcg gacaaaaacg agaatggccc cggtggcgaa
    15841 aaacataagg cctgccgtca gggggtgttc gagaagattc gttcccatct gactcgtcaa
    15901 ccagtcctcg agccagagct tataaccgac cccaataaat cccgccggga gactggccac
    15961 cgccagcaaa ccgatggtct tccaatcgtg acgaatcatc gcaacgatgc ggccccgata
    16021 atagaccagc acggccaaca gggtcccaaa atggagaatg acgttgacgg ccagtttgtc
    16081 aggcaaaggg gttccagata gatcgcacag atgggcgagg accatcacgt gcccggaaga
    16141 gctgatgggt aggaactccg ccaacccttg gacaattgcc aaaagaagaa tttggaggta
    16201 gtccatcatt tggaaaatgc gaccctggta agaatgcctc gggcgaagaa gactactctt
    16261 gcggacagaa tctccaagaa aaaaaagtgc ccttcgtagg cggggcaggg acctacgaag
    16321 ggctgcgctg tgagggtccc ctcacgcact tgtattattt cacgttcttc gtccatttgc
    16381 aagtccctgt tgagaacaat ccgttgagag atttgactaa tgcacaagaa tatcattttc
    16441 tggcgccgtt tcatcgatga ttgtgtcaca gaagtgaaca tcttccatcg cttttttgaa
    16501 aaatacttcg tctttatggg caccgctgat gggtttttgg gaccatctat cttatggtaa
    16561 acgcaggcgc ggtcacgcct cccagcctca cctctcgact gcacgagcag cctgaaccgt
    16621 cttcatttca caagacaaac cgcccaacgg tcaagcactt gcctgacggc ttcaacaatg
    16681 gcgccttagc gaccaaacgt atgtgtattg cccacggtac cagtttgttt tgcgcaagtg
    16741 tgcgaaccaa actgcaatga cgctcgggaa aacctgttcg gggagacctc agacaaacat
    16801 acaccagaaa gaggtcggaa ccaaattatc ttaaggaaat tgtcgcagaa aatatgtggc
    16861 cgtttgttac ctcattgcgc ctcgtcttgt cgcacacgtc gtctttttcc gaagaggtga
    16921 ccgaatcctt atagggcaat ccctcaccgg agtgagagtg actcatcggg tggtgattcc
    16981 cgtcagccgg gaacggtggg atcgcaatca aactcaacgg tcagaaaaca attcggcttg
    17041 ggctacgata tccacgcccg ttagttcgtt ccacgccgct agaatcttcg cgaaaaccgt
    17101 cccgggttga ccattcccga cgggccgacc attgaacgac gtcaccggga gtacacagta
    17161 cggtgtgctg gtgaaaaaaa tctctgttgc ggtttccaaa taagcgacgg tcaagggaca
    17221 aaactcaaag ggaattccca gcttgctggc caactcggtg acgaccttaa ggctgatgcc
    17281 cggaagcgtc cgctcgagcg gcggcgcgag taaacggcca tcgcgatcgc acatgaccac
    17341 attggcaatt gaagactcgg tgaccgagtc gtcctgatcc aaaagaatgg cccgacttcc
    17401 cgggtagcgt ttttggacct cctgttcagc cagataataa tgcatccgac tgcgaccctt
    17461 cagttcccgt ggccagcagg acgtgggaac ttgccgcacg gtggaaatgg ccgccgattc
    17521 tccggtgcgg tatttcttgg cccaaaggaa aaagggaagc ggataggtat gaacacacac
    17581 agttgggacg acaggacctg gccccgagta tctaatatat cggcccggcg tcacaaacac
    17641 agaaaccccg aggtcatcac gagggtcaag aagttgatga ttcgtttgcg cgacgtgctg
    17701 gatgacggag agcaattggg agggtgagaa gtcaatttgg acccccacga tttgtagaga
    17761 gtgaaacaaa cgttccaaat ggtcttccgc ccgaaagact tgccctcgaa atgtgcggat
    17821 ttgttcggca acggtcaccc cgagaacgaa tccgctgtcc acaaccgaaa tgcccagccg
    17881 cgactgggga atgtactcac cattgagaaa cgcgatcggc tcatccatgg tgtcaagggc
    17941 ctccatgaag gattgacggg aagtgtatta tttcaattcg atacgattgg ctaggcttag
    18001 agcaagtcgg gccattttgc catttgatcg atttatgatc atcttacttg cagtgggtta
    18061 ggggcggaca cttgctgggg cgtggaaaag ttaaggagtt gagagggact caccggtttt
    18121 ttggaatgcc cgacggagag tgggggcact caggtaggcc tcccatcggt gggtatgccc
    18181 aacttgtcgg gtgacatcca aaacttccag agaatccacc aagtctttct gcgtgcggta
    18241 gaatggccct tgccaaccga gggcactcgg cccatgacac gaccaaaggt gccggtacca
    18301 tcgcagagcg ggatcggcct tatttacggt cacgaagact ttaagggagg gactcgcttc
    18361 cagcgtttcc cggatggtgc gatcaaaggt ggccgtatcc atggccggtg caacgaggaa
    18421 tagtgtgaat gccggtgaat catgtgatcg tgcatccgac ccgggcaggt ccgacagtgc
    18481 gaccgacttt tgggtggcaa tttgccaagc ggcctgcatg acggtccgag caccgaaact
    18541 ataacccact aaattgaccg gcgcttgtag tgaatgactt gccaaccagg acgccaagac
    18601 tgacgattcc aaatcggcgc ggacggcctt taagcgacta tctcgagcaa tgccacgaac
    18661 cacgacacct gatggccatg accaaaggac aaagacgccg ttgagtccga cttgacgaca
    18721 cattcgacag aaggccgtca tgaatgcgag ggtttcgccg gaatcgaccc gattcccatg
    18781 aacgaccacc cataacggtt gttctttctc gatgtcttcg acccaatcat ccgttgtaag
    18841 ccgtcgaacc attccactct catctgcggc gaagaatcgt aggtcggtca gggcctctgg
    18901 cggattctta agcgtaccgg tggggatgga gcgggtgtcg atgaccaaaa acctgtaagt
    18961 acggtcgctc gtgttgccgt tctcgcccgg cttcagcggt gtgggcagag aggacaaggt
    19021 gacggtacgg tctcggaacg gataggcgtc cgagccaacc tcctgaatgg gcagagtggc
    19081 ggtatgctcg ggataagctt ccgcgctcac atcgtgaagg ctcttttctc gcacctgggc
    19141 agtagtgccg gcatcaacgg tccatcccgc aaatatggtt aagaccgcag cgatggctgc
    19201 aagcgtgtta ttgcgcgcaa ccttcatggc aagattctcc cattgtgaca gcatgactgc
    19261 tccaaacgtt agtgttttcc acggtgtcat ctgcgcggtt ccctagttag agcctatccc
    19321 aaaattccct ttccctctgg gagagggtta gggtgagggc aagccatcgt cgaagtcgat
    19381 cgagaaaacc ggcagcgatt cgagccaccc tcacccccgg cccctctccc gccgaacggg
    19441 cgaggggagg ttgtgggata gactcttagt gtcatcaagg catatggcgg gctctttggc
    19501 ttcctagcgc gaatgccatc ctccttttgg ggttgggcaa acacggacat catcgccgct
    19561 aacgtccgca cgtcacgcgg tagtgtaagc actcgttgag cggaggaaga cctcgtcaag
    19621 caagtgcccg aaccaacggc cggtcttgtg gttttggtcc actgtgttca ctcatagtgg
    19681 cgagcgtcgt taagtgctgg accattttac ctataactta cgaccgtttt ggccggtatg
    19741 caaaagggca aagaaacgtg gccaaaaagc atctgttgca tttgcacatc gccaattgtg
    19801 gtgtagggta ttcatggggg tgagatttca ccgcggttga cgaccgcaaa cacatttttc
    19861 agaaggagaa tggaaatatg aggaggatga ctgtcgttgc ttttggcttg ggcgtgatgt
    19921 tgttaacgat tgggtgtcag cgatgcaatt tgttccgccg aggagcatta tttgccccaa
    19981 cgacggtgcc gaccgctgtc atcgaatgcc caccggtcga tccctgtact gagaccgtcg
    20041 ttggcagcgc atgtgatccc tgcgtgccag ccacgactgt ggtgccaccg acttcgacgt
    20101 ttgttcccgg tccggcgggg tgattcgttt cctacaaaac gcctagcatt cttccagcgt
    20161 tcatgacggg accaagccat tcgggacctg gctgatggag tgagcgctaa aaaccagcgt
    20221 aggcgacaca accgttaggc gttccataat cagcccattg tggaggaaac acctccttct
    20281 ggcaaaccgg cgcgaggtcg tgtggtatgt actgcccgcc cgcgccggtt attcttatga
    20341 aagcttcaga gaatatccct tcgcaatcat gccggcgaac tgagcctcgc gtaaaatcgg
    20401 ccgctcacat gttggtgccg tctcatggta ggggtggcaa ttcgaggttg gcttcgcggt
    20461 gaactccgga atcgggaagg tcgattttgc cgcaaaggag gttcccgcgg atcattacgt
    20521 tctgtaaacc gattccgcga atggcaataa tgtcggccgg cggtacggct gggacctcgg
    20581 ttcccgaggg cggcccctga gaccccgctg cggcgactcg tattatacag tcggagacgc
    20641 ggctatcggt gacttgatcc aaaaggatgc cggccggacc acagtcggtg aattggcagt
    20701 tgatgatgtt aatcccgtga cagttctgga ctagtaaggc ggcttgtggg gcctgaacat
    20761 ctgcgaactg gaggccgttg agggtacagt cattacaatt tcgcaagcaa accccgccct
    20821 ggtagggtcc ctggtgcgcg taattggggt tctgatcgaa ggtgtttggg ccgaccacga
    20881 tgttccgcga atccgtgatg agaaggtctt ctgccacggc tccccaaaac gtgttcccag
    20941 taatgcaaac accccgggca ccaatgattt cgatattccg ctccacatca ctgagaacgt
    21001 tctccgcgat ggtcaaattg ccccacaatc gtcctttggc atcttggcca aagaagcgaa
    21061 cgttggtgga tcccggaact tttgaataat gctgaatggt acaccctgtg attgcgattt
    21121 ctgcatggcc gtactcgctt tctcgactat caatcaagat attcgctgtc ggctccgtct
    21181 cgacgctcat gttcgcttcg atatcgcacc cgctgacttg aagattgcgg agataacctt
    21241 ttcggacgac gattccccca gcggcattgt agctgatatg acatccgacg atgttaatct
    21301 gatggaggtc cacatcgtcc aggaagacgc cgatgccacg attttcgtaa atgtggcaat
    21361 ggctcagaat gacgttgcgg ttcctttggt gcaatcgaat accgtggaac gtctgacgaa
    21421 ttaatgtatt ggcaattgtc agttgcatcg tttgaatcgc ttcgatccca ctggcctcgg
    21481 gatgatcgcc gacgatttct attccctcga ccaccgggaa ccgttcgtgt tgccagacgc
    21541 cggggtggac ccgcttgggg tccgcggaac ccgtgtgcgt tcccagaaag cggatggcgg
    21601 gtccggcgcc ggccatctgc aatcgtgccg tgccaaaacc atggaaggaa acccaaccgg
    21661 tctcggccaa attgacttcc aaggggcggg taatgcggta agcccctggc gggaagacga
    21721 ttgcaccacc ccggccgatg caggcctgga tcgcggccgt gtcgtcctgc tgtccgtcgc
    21781 caaccgcccc ggctttccgc acatcctgaa actgcccgat ttcaaagggt acgtcagatc
    21841 ccgcagaatc aaccttgacc tgccctgaag tagcaacacc gacgggaata aacccatcat
    21901 gccccgggcc tgcggcgaag attgtgaagg gcggagcatt cacagaacca atgcccgggt
    21961 gagatgtcgc gaacccacga ggctgaccat gatcggcgag gacctgggtg attgagaaaa
    22021 gacataatgc cagtcccact ggcagtagac cttttccgaa aaacatcacc agtagatatc
    22081 tacttatgag catgccataa ccatcaatgc ggtcgtttga gttgtggttc atggtcttac
    22141 tcctagggta aaatgcggcg atttccgaaa cgctctgccg cccatcaaat aaagagggcg
    22201 gatgctttca actagcccag gatccgtgtt ttgccctaat ttatacccat agcggggttc
    22261 agattccacc tagtcatcgg gcaaacgagc ggatttctgc gaacatggag tggattcgac
    22321 cgaagtcctc cccccgttgc cgccattgtg gtgcgctatt gtggcgggtg actcgatgct
    22381 ttcctttttg aacgacctct ggcccaccgt gtagaatatg aaaatcgctc accgtccggt
    22441 gcagcgccac ggcggaaggc gacggctacg gatatgtggc tgtgccgctt tcccattggt
    22501 atgtacccat cggtgagcgt gggtttatca ttgagtgccg aaatctgagg accaataaca
    22561 tcgaaggaag ctcgagcgtg cagccctcac gatttggtgc catagccaca agatggaaaa
    22621 catgcaccct cgtttttctt gttctttccg ggctatgtct gggatgtgtc cggaatcgtt
    22681 cggcattgag ggatgggcaa cacgttgagg tagctgtgac gatcccacct caaaaatggc
    22741 tggtggaaaa aattgggggt gaccgcgtgg tgtgtcatgt tttggtagct gccaaaaatg
    22801 atccgcacac ctatcttgga accgatgccg atgcggcgag attggcccgc tgccggcttt
    22861 tttttaccat tgggcttccc attgagaagg caccatggtg ccaagcggtt ctccgcagcc
    22921 gtggtgtaag tgtggtctcg ctcggggaag aaggggaaga tgaccatgac catgaccatg
    22981 cggaccacca cggcggggag gaacagcatc aagatggcag cgacgacccg cacgagcatg
    23041 aacatggtcg tcatgtctgg cttagtcccc ggcgccttag ccggcaagca gagaaaatcg
    23101 ccgaggccct gtgtcgtgtt gatccatcgg gtgccgttga gtaccagaaa aatctggtga
    23161 agacacaaga agaattacgg gagttggatc gcgagttgtc acaaaagctc gagcctctcc
    23221 gcggtcagac gtttttcgtt tttcatccgg cgtgggaatg ttttgccgag gattatggcc
    23281 ttattcagca agcagttcaa gtgcaaggga aggagccttc tgatcacgaa atgaccgagt
    23341 tacagcggaa ggctcgtcag acgaagctga aggtgattct ggtccagccg caattctcat
    23401 cacgtgcggc cgaggccatt gccgaagtag caggattgca agtggtcgtt gccgatcctt
    23461 tgacagagga gatccccacc gtactgcggc aggttgcaga tctcctcgtc tctgcctatc
    23521 gtgaaactta tggggcagcg cgcacggaaa cccctagcac gggccagccc tgagaaggtg
    23581 tcgaaatgtt atcaaaccct tcttccgatc agaagttggg ttctgaatct gcgccccacc
    23641 catcagtagc ggcgagcgag gagatcgtcg tacaggtgga aggggtaagc ttcacgtatg
    23701 ctcaacaaaa tggggcgggt tcggcgacga agtgggtttt agaagatgtc tccctcaagg
    23761 tgttcgccga cgattttttg ggaatcattg ggccgaacgg cgggggaaag acaaccttgt
    23821 tgaagatcat ccttggcctt ttggaaccgc aaaagggatg ggtcagggtg ttcggaagaa
    23881 ccccgcgcga ggtacgtcac caaattgggt atgtccccca acacgcccgc attgatgcca
    23941 ccgtgacagc gacggtgctg gacatcgttc ttgcgggtcg aattgcccgt tcgcggtggg
    24001 gctttcgata cgggaagcga gatcgcgaag cggcctttgt ggccttggaa atgacgggtg
    24061 ttgcgccact ggcgcatcgt cgtattggga ctctctccgg tggacagcgg cagcgggtcc
    24121 tgatcgcccg cgccctggct gccgagccaa aactgctcct gcttgacgag cctactgcgg
    24181 gtattgatcc ctatgttgag caaaatctca cggacctttt gcatcaactg aatgaaagcc
    24241 tcccgatcgt ggtcgtgagt catgacatcg ggtttgtttc gacgcatctc aagcgcgtgg
    24301 cctgcctgaa tcggcggctc acagtgcatc gtgtttccga gattacccag gcaacgatgt
    24361 cggatttcta tcgcgaacac gtccacatgg ttcgccatct ggcttcttgt cctttggggg
    24421 acgccgggtg ccaacacgga tgccaatcat cgacggagtc agtgacgcgg gaggggcaaa
    24481 gtgattgact tcttccatga ttgggccgac aacccctttc tttggagtgg cctttgggca
    24541 ggtcttctgg ccggtttagc ctgcggtatt accgggccat atgtgatcac gaagcggatc
    24601 gttttcctgg ctggggcgat tgcccatatt gctgttggtg gaattggggc gagcgtgttt
    24661 ctcgcccaca agttcccgca gttatgtggt tcacttcagc ccattcaagg ggcgacggcc
    24721 gttgcgatca tctccgctat tgtggttggc ctagtcaacg aatactgggc cgagagaagc
    24781 gacacaatca tcggggcctt atgggctatc gggatggccg tggggctttt gctcgtcaag
    24841 ttcaccccag gttaccaagg ggaactgatg agttatctct ttggcaattt ggcctttgtt
    24901 agtcagcgtc aattgcagtg gatcggcttc ttggatgttg tgattatcct cattgtgctc
    24961 ctctttcaca aacaattcat cgccgtgtgc ctcgatcaag agcaggctcg actgcaggga
    25021 gtccccgtca cgttcatcaa tcttctcctg ttaagtgtgg ttgcgctcac ggtcatctgt
    25081 cttacctcga tggtgggcct tattttggtc atagcccttt tgagcctccc tgcggcaacg
    25141 gttgctcggt tcgtgaacgg catgctggcc atgatggtgt ggtccacgat tctcagttgt
    25201 tttctcacca cggtgccacg catcctggtg tatggaacac cggtcaatcc cgagtcggcc
    25261 atcgtgatcg ccgctgccgc tttgtatttg atgagtctct tcctcgcggg ccgaacggca
    25321 cgataatgct taccttagag cctatcccca aattccctct ccctctggga gagggttagg
    25381 gtgagggcaa gccatcgtca aagtcgatcg agaaaaccgg cagcgattcg agccaccctc
    25441 acccccggcc cctctcccgc cgaacgggcg aggagaggtt gtgggataga ctcttagttg
    25501 cgttcggtgc aggtgccacg ggtcaaagct ctagtgcctc tactgcgagg cgctgaaagg
    25561 cttctctgat ggccgaagca tcagcgttgg gaaaattggt tcgctggacg agcaagataa
    25621 agatcatctg ccgctgcggg tcgatccatc cctgcgtccc aaaggccccg ccatgaccga
    25681 acgtgccggg cgaaagcatt tctgtgaccc cttgaggctg ccgcacaata caccagccca
    25741 atccccagcc gttgccaggg gtaaaacccg tctgcaagtc atcggtttga accgtcgtca
    25801 taagccgtac cgcttcgggc gagagaatcc gccgaccaca cagttcccca ccgttaagga
    25861 tcatttggta aaaacgcacc aagtcacctg ccgttgagaa cagcccccca gacgggttgg
    25921 gggcacgaac gcctgcgtcc tcgatgagcc aagaggcgcc gccacctggc agaagttgac
    25981 catccgccgt cctttgatag gccttggcca gtcgctcacg ctgatgttca ttgggaacga
    26041 acgtggtatc taccatttcc aagggctgaa agatttcttt ttccaagaat tgctcgtagg
    26101 gcattccgga gacgacctcg ataatccgcc cgcacacact cagaccggga ctatagttcc
    26161 atcgctcccc cggttgaaaa gcaaggttcc ctttgacaat cgcctccacc gtggcctgaa
    26221 gcgtcgcctc cgttcgttgg cttcctgtga gtcccgaggt gtgggttagc agatgcttga
    26281 tcgtcagagg tcttgttaac cgctgcccct tgacggtaat ttccttgaac tcgggcaaat
    26341 accgcgaaac aggatcatcc aaggccacct tcccccgatc taccaatacc ataagagcgg
    26401 tcgccgtgat tggcttggtc atcgaggcga tttggaaaag agtatctttt cgcataggaa
    26461 tctgcttctc gatatccgcc caacccactg cttcccaatg gacaatggag ccattccgcg
    26521 caacaagggt aacggcccct gcgatctcgc gcgcctcgac aaactcctgc aaacggacgg
    26581 gaattttggc gaggacctcg gccttcatgc cgacttgtgc cggcgagaca aagggaagac
    26641 atctttcatc ttccgcaaga agcacgggag tactgcaaag gatgaggacc gcccatagca
    26701 ctgagattgc cattttcttt ttggcttgta ggatcatgtc aaagtccttg tttctttcgc
    26761 cgtcttgggg tcttgtcaga tacggcactc cgcgggggga agtgcgcgtt agcgctgcac
    26821 cttcaggatc tcgccgagcg aagctccaca tgaacctcgt ccatcgacag tccacaactt
    26881 agcatgcggt accgagaatg caagacccgg ggagggcttt gcaggcgtgt ttaggccaaa
    26941 tttgcgcaca aaaaagcccg tcgttgtgac gggcggaata ctctaaccaa tgaatcaggt
    27001 gccgcaatga aaaatttcac cgggacacac cgggacgggt gtcgcctacc atgggaacca
    27061 cccaaacctt gagatccgca tagatgtcgt gtcccaggtg tatctggacc gtataaagcc
    27121 caagttcctt gagcggtccc tgaagcttaa cctggtcggg gttgatgttg atattctggg
    27181 ctttaagggc ccgggaaatg tcgtccgccc cgaccgaccc gtaaaggtgt ccttcctcgt
    27241 tggcattagc ctcgatggag atgctcatct gcgagatatt ctcggccaat tgcttcatcg
    27301 tggagatgcg ggcacgttcg atctcaagca atttggcctt gtgtttttcc accatgcgtt
    27361 tgtggtgctc agtcgcgatg gtggcgagac cttgcgggat caaataatta acggcgtacc
    27421 cgcgcttgac ttcgacgaca tcccccgcct tacctaagtg ctctacattc tggacgagaa
    27481 ggagctggac accaccatgg ggccctcgag gaaggccacg aaggccatgc ttacgatttt
    27541 tcttcgttcc ttttccggtc gccatcatcg atcctctcaa gaagaataaa gactctctta
    27601 ggcttaggct ataggctcat atcgcgcgga gaaacatcaa aatggcacat catcctccgg
    27661 cggaaaacct gtctcatcca agacaccctc aaccgggcct ggcaccgggg gcttagtgcc
    27721 gccaggccgc gccccgctgt cgttctgcgc cccccgggtt cccagcattt gcattctctc
    27781 ggctacgacc cgcaatttgg accgtttctc gccgtctttt tcccacgtgt ccatgcggag
    27841 tcgcccctca atcagtagtg gcgagccttt ggtgagatat tcaccagcga cttccgcagt
    27901 ccgtccccag agtgtcacat ccacgtaaac gacctcatcg acccattcgc cactctggtt
    27961 ttttcgccgt tcattgacgg ccaaaccaat ttcgcaaacg gcggtgccgc tgggcgtgta
    28021 tcgaacatta gggtcgcgtg tcaagtttcc caccaaaatg acgcgattga agctcgccat
    28081 ggccgttccc ccctcactcg aatcgccagg gtgcccactt ccccacacaa aatccgccgc
    28141 atatgccgcg gattgtcaga gaagtaagat cactcctcaa cagcatctag gtcttcgacc
    28201 tcggaatcaa tttcctcgtc caaatcctca acatccactg tcnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnccgcagcc ggtaagtgcg tccgtaatct gttggacaac
    28381 cccgtcagga tcacgggcaa acctgttgga atcgaggata tacaggcctt cgtaaaagtt
    28441 ctgtgccaaa tgaacgctcc ttccaccaaa taccgcttcg atttttgtat cttgccagga
    28501 cagcgctttt tttatttcca actcagaagc tcaattcccg ccgttgaacc gtgtcatgca
    28561 aacgtctatt ccttcggcga cccatgtttc cacggcatcc gccgcctttc gataggcctc
    28621 acggataacg gcctcttcct cctttccgaa tacggaaagg acgaaccgaa cggaatcctc
    28681 tccgggaggc ggcttaccga ttcccacgcg tagtcgcgga atctcctccg ttcccattgc
    28741 gcggatgata tccgccaaac ctttgtgtcc cccggaggac cctcgcggtc gcacccgtaa
    28801 ccttcccaag ggaaggttca tatcgtcaca aatcaccaag acatcttgtg gcttgagctt
    28861 ataaaaatcg caagccgccc gcaggctcgt cccacttcgg ttcatatagg tcgtgggact
    28921 gagcaaaagc actttgatcc catttatcgt agcatcaacc gtttcgccgg aaaactgccg
    28981 acgcggtcgt acctctccgg cccagcggcg tgctaactcg gccaggacct cgtaaccaac
    29041 attgtgccga gttccaacat actgccgtcc aggattgccg agccccacga ccaatttcat
    29101 cggaggttgc tcctcggctt ggcctcggcc tgaaaacagc catcgccata tcagtttcca
    29161 agcaacgacc acgccgttgc cacctccgac tggtcaaaga atactcactc ctccgattct
    29221 tccgcctcag ccttctttcg gccgatgacc tcaggttcag caggtcccgg tacctctggt
    29281 gcggcttctt cttccacctc accagcgggc ggatgacaga ccaccacgat ttcgtccggg
    29341 tcggccaaga tcttggtccc ttgcggaacc gagaggtctt tgacggtgat cgaatcgccg
    29401 atattcaaat ggctgatgtt tacaaagatt ttttctggaa tcttgtcaac ttcgcactcg
    29461 atctcaatgc tgtggaggtg ctgagcaagc cgccctccca gcttcacgcc gggggattcg
    29521 ccgcgtagct cgatggctac ctcgctgcgg atgcgctcgt gcgccgaaac ccgcgtgaaa
    29581 tccacgtgga ggatctcttt cccccacaca tcccattgaa gttccttaat ataggcttgc
    29641 tctttgacag ccccatccaa ctggatcaat cgcgttccat gccgaacggc cgcggcgagg
    29701 gcatccccag gcacactcaa gcaaacgggt tccaatttgt gaccatagag gatcgccggg
    29761 atcttcccag catcccgcaa acgccggttg cttcgcttcc cgccaggctg tcgcaattcc
    29821 acaacaattc gttcggccat ctgtctaccc aatgcacaaa agacaaactg tttctgtacg
    29881 ctgacgtctc cgacccttcg gcatgatgaa gtccgaccga gggtcgtcga aggatgccgg
    29941 tcaagaagac caccgacctc gtcacgcgat ctgtgttacg tttgttgccg tgacaaaacc
    30001 ttgattttgg atgatctccc gaaaaattca agggtggtgg gcaaagagat ttgtctcctt
    30061 tcccgcaaag ccggccgctc tcgcaggtga ccaaattccc tcgctgcgac agcagtttgg
    30121 agttgccaat agccgcggaa atattgactg tctagcgata cgacttaaaa agtttgctaa
    30181 cggattcgtg acggtggatc cgcttcacgg cctcacccaa cagctcagaa actgtcaaca
    30241 ctttcaagtt ggggaggacg cgcttcgagt ctagggggat ggtgtctgta actacgattt
    30301 cttccgcagg cgctgctgcc agccgctcaa ccgcaggtcc acaaaacacc gcgtgggtcg
    30361 cggccaaata gaccttggca gcccccgcat ttttcaccat gtgcgcggcc gtactaatgg
    30421 agctgcctgt gctgatcatg tcatcaaaca ggagggcgac ctttcccttg acggattcgc
    30481 caatgaggtg agccgctgag gtactattcc cagaccgacg tttgtcaacg atcgccaact
    30541 tccccccaaa aacggggacg tgcaaaaggg tgcgcttgat tgacccttcg tccgggctaa
    30601 ccaccacgat atcctcgagg ggaattccca ggctcaaaaa ataggcgtct aacactggcg
    30661 ccgcataaag gtggtcaacc ggaatgtcga agaatccctg caattggggt gcgtgcaaat
    30721 ccatagccaa aacgcggtcg gccccagcgc gggtgatgag attggccact agtttggccg
    30781 tgatgggcac acggccttca tctttcctgt cttgtctagc atacccaaaa taaggaatca
    30841 ccgccgttat tcgtgccgcg cttgcccgtt tgaagctatc aatcatgatg agcagttcca
    30901 tcaggttctc gttaaccggg gggcaagtcg gctgaacgat aaagatatcc cgtccgcgaa
    30961 cgtcctcctc gatcttgacg gagatttccc cgtcagggaa acggccgagt gaaatccgcc
    31021 caaggggcaa cccgacatag tctgccacct tttgagccag ctcaacgttt gcccggccac
    31081 taaaaatctt catttcgtgg atcatggcac agacaccctc atcgaccttc cgagatgttc
    31141 tcatttcatc atggggcgct tttgcgccgg tgaccagcgg gaccgacttc gttggcctcc
    31201 aattgtcacg tgttctggct taatccaacg gccgagtgct actcacggct tcttgataaa
    31261 tttgaagcaa ctttctcgta cacgtttcga tattgaaatc gctgcgctcc atcaatgaga
    31321 ggcaacggtc gcgtgggatc gcgtgggcac tttgtgtcgc tgccgtgaca gcctccaccc
    31381 aggcggtgag tggttcatcg agcgaaagtc gccggataag ttgggggatc acgatagcct
    31441 cctcggggac cgcggaggaa gcgacgatag gcagcccggc tgcctgcgct tcgatgacga
    31501 cgaggggaag cccttcgtaa atggagggaa atagaaacaa gtcaaagagg ttcatcatca
    31561 aggctgggac atcacgccgg gagcccaaga ggcgaacacg ctccgttagg cccttttgct
    31621 caatccattt ttccacctgg cgacgaacgg gaccgtcgcc aacaaggacc aggtaggtct
    31681 ctggcatccg tgtcacaagg gcttgggcga gcgtcagcaa gaacttatgg ttcttctcgg
    31741 gcgacatccg tccaacatgg cctatgacaa aggcgtcctc aggtaggcca agagaatgtc
    31801 ggagttcacc gggccgttcg cttctttcgc ggaagggtgt aagatcaatg ccatagtgca
    31861 gaacttgcca acgtgggtca tcgcgccaat tctttccgaa aagagtctcc gccgcccttt
    31921 cgctacaggc cagtccgtgt gtcgcgtatc gcctgaccca tgattttgtc agacgggcga
    31981 caatcttttg atggaatcgt aaccctggtg cgagcaattc ggttgtatgg gagtgggcga
    32041 tgcggatcga gataccacag tgttgggcaa gccgaagttg aatcccccca ggttggacat
    32101 gggaatgaat gatgtcatac gggccccatt gccggtggat agctcgcagg tcatgcaact
    32161 ggccgagcgg atttaatggc cgatttgtga cgaaaagtcg tgctcccgct tgtcgaatcg
    32221 cgtcatccag ctcacctggc tggtcggtta caactagcac atccatgtga aaatgcgtct
    32281 ctatggcggc ctgcaagacc tggaggagcc aggtttcgat cccgccggga ttgaggcggc
    32341 ccgccaaatg gagcactgaa atgtgtgcac gcttcatgag tgatggggaa ctcttcaaga
    32401 ggaccattct cgcaagtcgc tcattgagat aacgtcttgc catcgtcatt tgattcccga
    32461 gctgtgggaa tcttgagaga gggatatcga tgcatcccaa ccacccataa taccacgtag
    32521 caaagcttca tagatcgtgg acggttttga ccaaaagact gtcaggtccg caagtggtct
    32581 ggcgtggctt ggcggacaag gagacgtcat ggcgatcttc gcccattttt gggatgagtc
    32641 catgcccggc gtcgggcctt tacgcaggcg ttgcggctgc cataaacttg atctatgact
    32701 ccgtcagagg atcttggatt aattctgtcc acgatttgct cagaacctcc gaattcggca
    32761 aaagcatgag gtgaaagaat gcaatcggcg cagccgtggg tgatttccgt aacgacagcg
    32821 acgttccagc gggatgtgct cgatcaatct cgaaacgttc cggtggtggt cgatttctgg
    32881 gcgccgtggt gtgggccctg ccggatgctg ggacccctgc tggaaaagat cgccaaagaa
    32941 aaacaagggg cgtttatcct ggcgaaagtc aatacggatg agtgccccga cctggctgcc
    33001 gcatttgggg ttcaagggat tcccgctgtg tttgctgtgc gggacgggag gatcgtcgac
    33061 cattttatgg gtgcgctgcc tgagactgat ctgcgggctt ggatcgatgg ggttttaccg
    33121 caaagagatg aacagttggt ggcccaggcc gatagccttg ccgagcaaga cccacaccga
    33181 gcagaggagt tatatcgcca ggccctggcc gaaaatccaa aaagtatccc tgcccgtctc
    33241 ggtctagcca aactcttcgc cgggcgccgc cagttcgagg agctcaagac gatcctgcaa
    33301 gagttgagtg atatgggcgt aagcggtgaa gagatcgaac ggctgcgcgc atccgtcgaa
    33361 tttgcggaat cgacaagttc aactgacgaa attgcagaac tccaaaggca agtggcaagc
    33421 gatcctagca atcacgcctt gcgtcttcag ttggctaagg cactggcctc ccgtcagcat
    33481 tatcaagagg ccctagacga ggcgctggct gttgttcagg cggctcaagg cgagttgcgc
    33541 gatgatgccc ggaaattaat gattcaggtt ttcagtctgc tgggtaatga acaccctttg
    33601 acggctgaat accgccgacg gctgaccatg gcgctgtttt aacaccatgg attggtgaaa
    33661 atcacatctc caagctgcca tcctggaagt gaacctggcg aatacaaggt aatagggcaa
    33721 cgggaatagt cgcattcgta aaaccggtga atacgcaacc acaaatcagt attccaagcc
    33781 catccgccat tcgcatccgt ggggcgcggg tccataatct gaagaatatc gacgtcgata
    33841 ttcctcatga acgtctggtg gtgctcagcg ggccaagtgg ttcaggaaaa agctccttag
    33901 cgtttgacac catcttcgcc gaggcccagc gggcatatct cgaaacattg tcgccatatc
    33961 ttcggcagtt ttgcttacag ttaccacagc ctgatgttga tctgatcgaa gggcttcccc
    34021 cggttgttgc catcgcccag tgggcagggg ctagccagcc gcgcagtacg gtgggaacga
    34081 ttacggaggt gtatgacttt ttgcggcttc tgtacgcaaa ggtcggggtc ctccactgcc
    34141 cccattgcgc tgttcccctt ggctcacaat ccccggaaaa catcgcgacg gaattgatgc
    34201 gcctaccgga aggaacgcgt gtgatgatct tggccccgtt tgttttccaa aagagagggg
    34261 aacacaaaga ggtatttcgt cacattcaga agcttggttt tccgcgggca agggtggacg
    34321 gagcgatcgt cgatgtcgaa gaccctccct tactagatgc cagtgcaacc cactccatcg
    34381 aggcggtcat cgaccgagtc attgtccgtg ctgggatcga ctctcgactt atggaatcaa
    34441 tccatctagc cctcaagttg gggcagggcg tgttgatcgt gtgccacgaa gagtgcgatt
    34501 cccaaggaga aacgaggtgg gtcgatcgct tttttagcac gactcggcat tgtgctcaat
    34561 gtggggaaag ctatcccgaa ttagaaccgc gaacattcag tttcaacagc ccttacgggg
    34621 catgtgcccg ctgccagggg ctgggtgaga ttgagcaatt cgatcccgat ttagtgttgg
    34681 attggaacaa agggttgggc aacggggccg tcaaattgtg gactgacccg cgggagtcgc
    34741 tcaagcatca cgaaaaaaag ctggcccgcc tcatgaaaat ggggaaattt gattggcaca
    34801 cgccgctggt aaagattcct aaggagtacc aaattcagct catctacggg caaaagccgt
    34861 cccacatgca aggggctatg aacccgaccg atggcgagac ctctgcttcg cggacggaag
    34921 cagcaaacag cgggcggtcg ggatttttgg gccttctgca tctgctggaa gtggaatatg
    34981 agctcttggc cgacggtcga aaacggcgtg cctgggcgtc ctaccgtaag ccggtgacct
    35041 gcccagactg cgggggggca aggctggggc ccatcggacg ctcggtcctt gtggccggtc
    35101 tgccgcttca tcaattgtcg gcaatgaacg tcgatgacct ccaaagctat ttggcccaat
    35161 tggccttctc cccgcttgaa cagatggttg cggagccgat cttacgggaa gtgcagtcgc
    35221 ggctgggatt cttgaaacaa gtgggactcg ggtatctctc gctcaatcgg ccgattgata
    35281 cgctgagcgg tggcgagttt caacgcgtac gattggcctc cggattaggg gcggccctga
    35341 cgaatgtgtg ttacgtcctt gatgagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35461 nnnnnnngtg agcggatctg ggaaaagttc cctcctcgct caaacacttg tcccggcttt
    35521 gaagaaagaa gtgatgaaac tgccagccag cccgggacct tattccaggt tgcggggtct
    35581 gaagcacatc gatcgagtgg tggaaatcga tcaaacaccg attgggcgat ccccgaggag
    35641 taaccctgcc acttacagcg gcgtttttga cgagattcgt aaggtgtttg cagagacccg
    35701 cgaggccagg cgacttggat tcggcccatc ccgtttcagc ttcaacacac ctgggggacg
    35761 atgcgaactc tgcgaaggat tgggtgtgcg aagaattgaa atggatttct tgccagatat
    35821 gtacaccatc tgcccacgat gcctcggtcg gcgtttcaat cagcagacgc tggaggtgcg
    35881 ataccgcgag aagacgattg ctgatgtcct ggagatgtcg gtcagcgaag ctcggcagtt
    35941 tttcgagaac tttccatcca ttagccgcct gctttcgtcg cttgacgacg tcgggctggg
    36001 atacttgcgg cttggacaac ccgctcacac cctctccggt ggagaggccc aacgtatcaa
    36061 attggggacc gaattagggc gggtccagca tgggcggacg gtttatgtca tggatgaacc
    36121 gacgacgggc ctgcattttc aggatattca gcgcctgttg atggtcatcc acaggcttgt
    36181 tgatgccgga aatacggtca tcgttgtgga gcatcacttg gacgtcatca agtccgccga
    36241 ttggattatc gatctggggc ccgaaggggg gcgagccggt ggggagatcg ttgccgtagg
    36301 tccacccgag gaaattgcca aagtcgaacg tagtcacaca gggcgttacc tgaagcaaat
    36361 tttgaactcg tgcgcgagcg attcttccgc acaatgagca agcgacagaa ggccttcggc
    36421 aaaaagcaaa aaagctgccc cgccaacgga cacgtggcgg gcagtcttga tttgcccttc
    36481 tgacgattca tttcgtcagg aatgttgaca ggatttcggg atgggcgttc gatgtgccca
    36541 tctcacgcga tattccccgg agcggggcct cattgagatt tcgagtggct cctttgccaa
    36601 acaaaaaggt cgtcggcctt gcgaccgacg acccggctcg gaggagggac gtcatgctaa
    36661 gcggctattg ggtgatcccc agtttttctt cttcctcttc ggcgatgatg atgcgtgggg
    36721 tgaccatcat catcaggctt tgggtttcac gaccgacgcc cacgttcttg aacagtcggt
    36781 tcacataggg gattttgctc agcagaggca caccgaattc gttgcgccct tcgctaagac
    36841 gtttgatacc accaaggagc acagtaccac cgtcgggcac actgacagtc gtcaacacgt
    36901 agaaggacga gactgtcggc aactgcacag tggtgccgga tcgggtggtg ctacgcgcat
    36961 tcgccttctt ggtggcatcg ttgggttgat cctggttgcc ttccgccgaa gtcgtctcgg
    37021 tggtggtttc ggaacccaca aacgtaaact cgttaacttc cttgatcttg gcgaagtacg
    37081 ggatgatcgt catccgcacg aagcgccggt cctgcgacac cacgggctgc accgtgagga
    37141 aggtcccctc attgaccata gttacaacgg gttgctgggc tgccgcaaag tctcccacca
    37201 ctggcaccac gctgataacg aaggggcttg tggattggtc agccaccata ccgtactgac
    37261 cgttgaagag ggtgaccttg ggcgcctgga ggacgttgga gcgacgatct ccctgggcgg
    37321 cttcgatgaa gaagaaggct tcaatgtcgc tgaggatcgc gaacccaagt gaagcacccg
    37381 ctgtgggatc gtagccgccg aactggggca ccgccaggct atagctgcct tgtcggaagg
    37441 ggatgtccaa gtctgccgag aagacgttgg gggccgacag accgaccgtc acacccttgc
    37501 cgttgtcgcg gtcccgcagg tcacgaacat cgagctgttc gtcggcatcg cctgtgggat
    37561 ctccggcttg gacgatctgc ccgaatctca cgccgtgacg gccatcgatt ttgtcgtcga
    37621 tgtcgaagtc gaagtccacg ccgatgcgtt cgaagaagtt gtcgttaagg gtgatgaacc
    37681 gtacctcgac ggtgacctgg aggtcctgca tccgccgcaa ctgttccaag agatcggaaa
    37741 tctgttcatg gacctcttgc gtctggctga tgacaagact caggtttgtc gggaacggtg
    37801 agatcgaacc ggggccaccg accgaatccc aactgttcgg cgaaactgtg gtttcgatca
    37861 ggtcgatcaa gctgtcgaag tcggccagcg ccgcaccacc catgccacct ctgcctgtcg
    37921 agaagggagc ggtgctccgg ccaggtgaag cgtgggcagc cgccgaggac atctgagcga
    37981 gaatcatggg attcactgcg gcggtggcag cggaaccatc tcggcccgca accatggcaa
    38041 ccggcgagat ggggctggca ttggggccga tgccaagctg gcttaaagcc tgctggtaag
    38101 aggcgtcaag atggagccct tcgccgacga agttcggaat gggcgacacc aggtccgcca
    38161 cgttatacac gaccgggtac accgtaccgc gtcggtattg ctcgctggtg atcttgagca
    38221 cgtcgtcctt aatcacatag gtgaggtgga gcggctccag gatgagatta agcgcgctct
    38281 tgagggaaat ctgttgcgtg aggttgatcg tgaccggggc atcggtcgtc acaccttcag
    38341 aagccaaccc ctgtggatca atgtgcaggt taatgtcggc gagttttgcc agataatcca
    38401 agacccgcac aagtgccgcg ttttcgaact ggagcatgac cggggtctga agcttccgct
    38461 cgatctccaa ttcttgctcc gagcgctggc gaccggcttc cgtaagtttt gatcgccgct
    38521 tggtcaagtc agcccaggac gtagcgtcgg gcatgatgta gggatttgag tcatcgaatg
    38581 ggatggaagc ccgatccacc gatgccagtt gggccaggaa gccctgttcc ttttcgtctt
    38641 tgatcgccat gctttcgctg tattgccgga tgaatcgagc gttgaggacg agttgcttgg
    38701 tcaccggatt ttcgggatcg agctccgcag cctttttggc gacgacttcg gcttcagcga
    38761 accgctgctc tcgcatcaac tggttgtact ggtccaccat ttgagcaagc ttcgtttgcc
    38821 gatcgcggag atcgttcgcc tcttggctaa tcttttcgct tatctcttgg tttttctgct
    38881 cacgttcgat ccgcggccga ttctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39001 nnnnncgccg tccgggccgc gtcttgctgg aggacgtcct gcacacgttg cgcggtcgcg
    39061 gggtccaact cgtggaggta agccgtagct tggcggaagt agtcgatggc aacagcataa
    39121 tctccgcgac ggagggcatc ttcggcttgt tggaccagca tcattccgcg gctggttggg
    39181 gcaggctgag atgtggaggc cggaacattt cgggtccagt cccgcgtggg atcatacacc
    39241 gcagctgctg cgacgttacc gctgtatggt ccggaggcgg ggaccaccgc ttgagcactg
    39301 gctggacgaa cggcaccggc ttgcttggcc tgttggatgt cgctgatcaa gcgggctggt
    39361 gaatcttcgt taggagaaaa cacactttcc gggagattga gggccaggat ttggtgggca
    39421 agagtttcgg cagtgctcac gtcagacatg gccagggacg accgcgcttg tgccaggagt
    39481 tgcatggccc gctgcttctg agccgctagg gccggattca gcccccggtt tcgacgttca
    39541 ttggcggctc gttccaaaat cgtctccggc ttcatttcga acgggctgaa ggtcacaccc
    39601 tgttgggccg caagttgggc caggcgttcg gcctcgtcta agtcacccca acgcaacagg
    39661 ccgtcggcct cctcaaggag caacttggca tagaggcgtc gccaaccctc tgtttggcct
    39721 cgttccgcct tttgcgaaag gagattctcg tgcgtctgaa tgagcgcttc aacccgatcg
    39781 ggagaatcat cgtatcgacc gtaggtcaat cccagagcac gaacttgctc cacatagcgg
    39841 cgagccgtcc gcacatcacc gagggctaag gcccgacgtg cctcgagaag ccattggtcg
    39901 ctttgctgct gggcaggcga cttttggctg gcgttcgccg ggggggcgac ctgtgtcacc
    39961 tgacctacag gaggaagaga ggttacggca ccaggtgttg ccccggcaac ttgtccacct
    40021 gcacgccgga tgtcctctgc caatttgctg ggcgaatctt cttccggggc gaaggtcgca
    40081 ggcaatccgg ccgccatttg gcaccattga gccgcctcac tcagatttcc gcgggctagt
    40141 gccattcgcc catttcgcac atagttcttg gcctgcgtct ttggatcggg cgtttgaccg
    40201 agtccgcggg catcaaaggg gctttgcggc atggtttgcc cggccggctg tgcttggcgg
    40261 gcactttcca aggccttgcg gaccttggca ggtgtgtcac ccagataaag cggcggataa
    40321 ttcacaccca actgttctgc ctggtgaagg agttgctcgg cgccattgag gtttccttcg
    40381 gcaatggcct gccgggcccg agccaaaaga tcatcgcatt gccgccggag ttggtcagcg
    40441 tttgccacag ggccgttgtc tgtaccttgg ccgactcctt ggcctaaggc tggacaattc
    40501 aaaatgagga acgccagaca gaaggccacc attccctggt agattcttac acgcgcgttc
    40561 aacccagttc tcctttcgag atcaccaaca agccgtccgt tacttgttcc tgtcttcatg
    40621 gaatcctgag accctgatat gcgtcgattg aaaaacggca tagcggtatc gcctccatgc
    40681 gattccctac cagccgcaat cactcaccag ctccatccct ctggagcaat gacctttggt
    40741 gaggattggg tattctccac cgcgtcggca agtctacgtg cgggattaat accgacggtc
    40801 acatgttggg tcaagatcga atcgtctggt ttctctcgaa cttcgttttc acgtgcggcg
    40861 ttgtcttgtt ccccctgaac taacctcttc tcacagctcc ccggtgttgg tcattcgcgg
    40921 tgaatgcggc ctgagtcttc cacgtttttc ccaatcgtcc atgaccaacc tgcgcgtctt
    40981 ttcacaaaaa tggtggcgaa gaataacggt cttctagccc tgccgtcaac accaaaaatg
    41041 aaaagattcc aggaggtgtc gggttttatc cggtcgatgc cttccgaatg acaagacaga
    41101 ggaggcgaca ggcaagacga agaaagagaa atacctcgca ggcaaggtgc taaacgtgtc
    41161 gaacggggag ccgagaaaac actcgagtcg gccgtgggga aatagtcaga gagaccagaa
    41221 ccgcggttag ccacccaaac aacgtgcttg tggttattgc ttgaaccgtc gttggattac
    41281 tgttcgactt gtgatcgttc agcccgcaca acttgttgca acatccaagc gatgatccca
    41341 accccgatcg cgaaacttac gagaaacagg gccattggtc cccaatccgg cttggttgcc
    41401 aactggttta aagacactag tggcgtcaat tctatgtttt ggacaagctg gcgactgacg
    41461 gcgttggtgg agaggacaat aacttgggcc agactggcta acagcacagg tccttgggaa
    41521 accccacggt accgaatcca caaaagtgtg gccacaacca ggcccggtga ggcacccgtg
    41581 aacagcgtga gcggtaatcg ccagtcactc cacatgtaag aaaaaacgga tttcggccac
    41641 gtcccgaaga cgtagcccgt ccctgcactt gcgtacacca cagccccaac aataccaatt
    41701 aggagactga acttagcaag ccagcgacga tattccaacg tccctcgctt gtcgaaaaat
    41761 accgcatcga ccagcgacca aactgccgtc gtcataagtg ccattccgaa caccatgggc
    41821 cagcggtgcc acaatgaggc atcaccgaag ttgtggccgg ttcccgtagc agcgccggcg
    41881 acctggttgc tgaaccaaat aaacttccac cgctccggtg cggccatgag actcagcccg
    41941 ttggaaaaca aaatggcgat gacaatgaat actcctgctg aaatccaacc ggctagcaaa
    42001 cgccaggcgg gtattggtct ttcctcgtcg ttcagtccga acgcaaacgc gtaaacgccg
    42061 taataggcaa tgattaacag agcgatgatc gccaaccaga accaagccgt gagaatggtt
    42121 gccggataga aggcccaggg gtaagccacc tggataaaaa gcagtggcac gatcccaaaa
    42181 ttgatcccaa aggcaacgat gatcggcatc tgccgcccca gtcgtacggc ccaccgtcgg
    42241 ggagatcctt gccagcgaga gaaaaagacg gccaaaatca ttccggccaa ccaaaggttc
    42301 atcggaatca tatggagaat aaaccccagc accttaaaga actgaatgaa ccacataggt
    42361 gcgggaagag cgggcaaact ggagacgtcg agaggttcga tcacggttga ttctccaatt
    42421 tctcgagact cggataaatc ccacgcggcc gctcgggggc taatgtcagg agataatcgg
    42481 caagcaactc ggcctcttca ggtgttcccg accagggcgg catgaagtaa tgagtctcat
    42541 ggagatggcg aatcatatcc acgagcatgg gccgagggcg tgaaaaaaga atcggtccca
    42601 ccgccgaata tcccgactcc gctgcatggc agtcattgca atgatgcatg aaaatgacct
    42661 gacccaagtg cctccgatct gattccggta gggccaaaag cttttcaggc atgatcttct
    42721 tttcttccct ctcttctcca accacttgcg gataatgctc catcacataa gcccgggtcc
    42781 aacgtccgct ttgaaggaat ccattacgac gaagttcggg gacctcttcc tccagcacct
    42841 gattgcccag cacaacacca tacacaacat agggttttcg cactgcctcg cgaataaact
    42901 cgccagtgct gaaggccgct atcccgaaca agagaagcga cgcggcaaag ccgggcgtca
    42961 tccaacctgg attccggtat ggtccgaaat acagcaggac aaaaacggcc acggtgagcg
    43021 cgaaaatgag ggtggtcaaa accgtcaaca cggcggcggc ttccaaagcc agacgggcag
    43081 attccggcaa gaacaacaac cacccaattc caccaatggt aatcaggata gccccgacaa
    43141 atgcaggtcg ggtcgaacgg cgaacaatta aagagcggac ctcaccgacc ggcaagagga
    43201 gtgagctgtg cagatagacg tacaaggatg tcagcaggat ggccccgcct gttcgggaga
    43261 tgacctgggg cagaaattgc gggttgaaaa aacccgtcca gaaggacgga gattccagcc
    43321 agcggccagg ggatagcatg aatgcggtaa tgcccgtaat caacacaagg ctgatccagg
    43381 cggcagcggc ataaatccac gcgacaattt gatgagtccg aggtgccagg cggccccagt
    43441 agtagtaaaa gataaaggct gccgtcagtt caatgacgaa gaaaacccat tcggtcgccc
    43501 aaccaaagac aaaggtccgg ataagcactt ctgtggccag cggggaagcc aatccgattg
    43561 tccaccaaat gcccacgccg gtgatcgcgc caaaaacaac ggtgagcagg atgaaaaagg
    43621 gagcatgtcg gcggaggtag tccaggtact cgcggttgcc ggtgcggtaa gcaaacgatg
    43681 tctcaacggc gaaaaacaag cccccaccaa ctgcgtagtg ggccacgagc acatggagta
    43741 ccgcgatcac cgcgatgagc attggggacg tcaaaaccgg cacataccac cagggataat
    43801 gcatacttta tgtttcccaa aaccgctggc gccagagtgg aaaacccgta aaacaacaga
    43861 cgaaacagta aaaaatattg ttgctgctgg ggcgacgtta agcaagccct tgcatactaa
    43921 gcgagggcag agggaaattt cgtggaggca ccgccgggcg gtgccctgcc gaattgcggg
    43981 gctcaccctg tcggcgacgc caccgagttc gtctgctcgc actcgcagca cgcccggagt
    44041 cccagtttgc taagcatcca aacggccggg caccagttgg taaaggccga ctggattagg
    44101 ttgaggccca cgaaggccgt cagaattagc caaagaggag aatgaaaata ggctagcgcc
    44161 gcactcaatg tgacgacgac acccgccatt aagcgtagtc cacgttcgat ggtcatattc
    44221 cgtaacctcc tttcaatgca tcacttaggt tcgtcttcaa tcggtggact taccgagcac
    44281 ccctcgtcgg gagcacgttc ttgggtagct ggagaagatt cctcaaacgg tgtggaacat
    44341 gccgatggac cgcaacaacc cccgctcatg caagttggaa tgccgagggc cgggagaatc
    44401 cacttattaa gaataatcca accagcaaaa atggcaataa tcaagaccaa atcgcgactc
    44461 atttttgctt ccttctcgag tttttactgc gttaggacac agactggccg aaccgctctt
    44521 gccatttttt cttcagacat tcgtgtagtc tggcgtggac ttccggcgga agttcatagg
    44581 ggacttcacc tttatataag gtgatcgttt tgcgaagggt gtcgatgaca actcgacagg
    44641 ggttgcaccc ctcaagatgg cgggaaagct ccgcgcatac ttgggggtcg atctcaccgt
    44701 cgatataagc atttagagcg gccagcagtt catcgcattt cattgaccgt aacctcgccc
    44761 aaggtatttg ctactactcg gtttgatgct ccgcatcggg caaagttaca agtttaacgg
    44821 aaaacatgtt gccgaagaat cgccgtacca ggtgaacccg gcgccgcagg gggcgaatct
    44881 cgccaacaac gcctaagctg ccggcttacg ctcgccggca cagagaatgg ggcaaagcgc
    44941 gttgtgtcaa tagtccgaga ccaaagtccg tggcatctag cctcttttgg tgacgcgcca
    45001 gataagtaca atgggcagcg ttaacccgag cctgctgctt tcttgattct gctgtctgac
    45061 atgagggtta cgatatggct ggctggatac ttaatctccc acccaccggt gcgtggtaca
    45121 actggcgtcc gggtgttccg catcggaagt cgaatcttgc tgtcgatgtt ttttgggtga
    45181 ttttatggac ggtgataagc accttgccgc tgcaaccact gttcgcggag agcggcttgc
    45241 gattcgccag acgggttatc aatccagagt cggagtttac ggcctgtgcc atatttgacg
    45301 tcaacggtga tgggcggttg gatatcgtgg ctggcgggtg gtggtacgaa gcgccgacct
    45361 ggagaaagca tttcctccgc gacgttcccc aaattcgcgg tcgttatgac gattattcca
    45421 atctttccct cgacgtaaac ggggatggtt ggcccgacgt tatcagcgcg aactaccgca
    45481 gcgaaagcct cttctggatt caacaccctg gaccggggct cgggccttgg caggttcatc
    45541 tcatcgaaaa acccggtcct atggagaccg gccgtcttta cgatgtggat gcaaacggca
    45601 cgctggatgt tttgcccaac gggatgaaat tcgcggcgtg gtgggaattt cgacgggagg
    45661 atacgggaaa tgtgcggtgg atccgtcatg atctgccccg tgaaatcgcc ggtcacggaa
    45721 taggctttgg cgatgtcaac ggcgatgggc ggggcgatgt tgtaactccc atcggatggt
    45781 gggaggcacc gaaagatcgt gccaacggtg agtggatcgc gcatcgtgaa tgggtactgc
    45841 accgcgatgc ttcggtacca attatcgttg aagacatcga tggcgatggt gatagcgaca
    45901 tcatgtgggg acgcggccac gatgtggggc tctattggct tgaacaggca agagatggcg
    45961 actcccgtcg ttggacgttc cacgtgattg atacaagttt ttcgcagatt cactctatcc
    46021 tcgtgacgga cctggataac gacggccgtc gtgaattcgt ggccggcaag cggtacctag
    46081 gccacgaagg gcgtgatccc ggtgagtgga acccgctggc catttactgg tatcgcttcg
    46141 atccgggacg aagaacattt atcaggggcg ttgtcgatga gggaggacca gtggggtttg
    46201 gtctcgaccc taaagcggca gacattgacg gcgacggtga tatcgatctg gtggcttctg
    46261 atcgcagtgg cctgtatctg ctagagaatc tgttaaaaag tgacccagag caaacgagtg
    46321 acgtcctgcc tccgtcctca gaggaaggcc cctggaccaa ggaggatcat cgcaatgtgc
    46381 ttctctatgt agatgagaat aacgagacga aaacggtttc gtccttgaag gagtggggca
    46441 ttcgccgcgc ccacatcctc aaagggatga tggccgcgat gggcccactc cccggtccgg
    46501 agcatcgcgt gccgctcgat atcgaaatcg tctccgaaga gatgacggaa tcctatcgtc
    46561 gcctgaaact gacgtatgca gcggacgagt cggaccgggt tcctgcttat ttgctcattc
    46621 caacgaggcc ctcgcaacgc aagcccgctg ttttgtgttt gcaccagacg acgccattgg
    46681 ccaaggacga accggcaggt acgcatcccg atgctgcttg gccttatgcc catgaactcg
    46741 ccctaagagg ttttgtgtgc atcgtgcctg actatccctc ttttggcgaa catgcttatg
    46801 atttcagggc tcacccggaa tacgccagcg gaaccatgaa ggcgatttgg gacaacatcc
    46861 gggcggttga ccttttggaa tctcttcccg aagtagatcg tgatcgaatc gccgtgattg
    46921 ggcattctct gggcggacac aatgccttat tcaccgccgc atttgagcaa cggattcggg
    46981 ctgtgatttg cagttgcggt ttcacaggct ttcaggatta ctacaaaggg aagatcgcac
    47041 cgtgggcaca ggaccgttat atgccgcgga ttagcaccgt ctatggaaat gatccggaaa
    47101 aggttccttt tgacttctac gaggtgttgg ctgccttggc cccgcggccc gtatttgtca
    47161 atgctccttt gcgcgatgcc aacttcgatt gcgatggggt gaagaagatt cttatcgcga
    47221 tagaaccagt ctatgcactc cataatgtcc gcgatcgaat aaaagccgtg caccctgatt
    47281 gcgaacacag ctttccggcg gagatccgcc aagcagccta ccaatggttg gaagaacaat
    47341 ttggcctggc gaagtccaca aattaggcca gagggggcgg ccgcgtcata gctgggagca
    47401 aacttggtca tcgacaaggg tactcccggc gactatactc tgtggtacgt tgcgtgaccg
    47461 ggggagctgc aaacttcttg gggtgtttcc aaaggtaatg catagggcag aagcccacgg
    47521 ggaagccgca caaatgagga gtggaattaa tagcagggcg cccttcaacg taaacagtga
    47581 agattgtctg gattttggcg aataagggaa gacgtatgct cgcaaagaat gatctctcgt
    47641 accctgttat acgaagatta tcaagtgagg ttgtgcgtcg tgtaggactg ttgtttgtat
    47701 gggttatcct tttccctttc gcagggagca tgggccaggc ggaggaacaa tatcttggcc
    47761 cctggcaagt gcgggcgacg gccgacggat cacgggtcct cgtgttgtgc aaagacgccg
    47821 ccaagattgc gatcatcgac ccgaatacgc aacagatcgt gggttggatt gcgacaccgg
    47881 cgcagccaag tgattttgtg atcagtcccg atggtcaaac cctgtttgtt tccgcgggag
    47941 tggctgacgg aatcgttgtg gttttggaca attccggcaa gcgtttgaaa gagtttcgcg
    48001 ttggtcactc tccttgtggg ctctcgttgt caccggatgg tcgcttcctg tttgtctgca
    48061 accgatttaa gaacgacgtc acggtcatcg acctgctgaa agggcaaacg atcgcttgcc
    48121 ccaaggtgat tcgagaaccg gtttgcagtg cgattactcc cgatggtacg aaactcttcg
    48181 tggcgaatct cctgccatat gaccgctgcg atagctatga tgtggcagct tctgtcacgt
    48241 gcatcaatac gcaatcattt gaaacgaccc acattcgact cctcaatggt tcaaccgatg
    48301 tgcacggttt gacggtttca cccgatggca ggtgggtctt cgtcgttcat gtgcttgctc
    48361 ggtatcaaat gccgacgacg caactcgaac ggggatggat gaacactaac gcgctgacga
    48421 tcattgatgc agccgcactg aaacgagtga acaccgtcct tttggatgat gtggatttgg
    48481 gagcagcact gccctggtcg gtggtgtgtt cacgggatgg ctctacggtc cttgtgtcgc
    48541 atgcgtccac agatgaggta acggttgtta atgtgccagg ggtattggaa aaacttgcct
    48601 cactgccggc gacacaggaa gaagccaagg gtttggggcg gatttacgac ggctctcaaa
    48661 cctccctcag tgcggtggat gttccaaatg acttatcctt ccttgtgggt cttaaagaac
    48721 gtatgccact ttatggaaca ggaccgtatg attatctggc cagtcagaat gaaaagatta
    48781 tcaagggggc aagaggcctg gccgcagtgg gtaataaggt gtttgttgcg acctatttcg
    48841 gggactgtgt gacggtcatc gatttgtctg cttcaaaata tcgtcgttta agctacatcg
    48901 cgcttggacc tacgccaaaa ctgacccaag agcggttggg cgaaatgcac tttcacgatg
    48961 ccagcctgtg ctttcagcac tggcaaagct gcgcgacatg ccatcccgac ggtcgaattg
    49021 acggcttaaa ttgggacttg ctcaacgatg ggatcggaac accaaagaat aacaagagcc
    49081 tgctatttgc ccatgttacg cccccgtcaa tgtgggaagg tgtgcgggca acggcggaag
    49141 atgccgtgcg ctcgggaatc cgtcacattc agtttgccgt tcgtcccgag gaggatgccc
    49201 aggccatcga cgcttatctc aaggctctca aacccgtacc cagtccgtat cttgttaacg
    49261 gtgaactcag tgaatcggcg aaacggggac gtgaattatt cttcagtgac cgcctaggct
    49321 gtcggcattg ccatcccgaa ccgctttaca ccgacttgaa aatgcacaat gtgaaaagcc
    49381 gggggaaatt ggatcgccgc gatgaattcg acacacccac gttgatcgag atttggcgga
    49441 cggcccctta cctccatgac ggtcgttatc tgacactgga ggagttgttc cgggaaggac
    49501 aacatggtca gacggtgggc gatgtggcgg ggctatcgga taccgaatta cacgatttgt
    49561 gtgaatttct gctgtcactc tagcactgag gaggtatgtg cgggtagccg gcaaccgctt
    49621 ctgctatcag agcatggcat ccgtcgtggg atccagatgc tgcggtgttg gggcaagggc
    49681 gattgattcc cagccatagt taggagagtg gctatttgag acgaagttgt cccgtcagcg
    49741 tggagaccga catgtgacgt ctcgcgtctg agcgagttct gccaggaccc attcaagttc
    49801 cggcaagaaa acgggcttgc tgataaccct tgtcgtgcct cgggcaagaa gcggccaaac
    49861 ttgctgccag cggggaaaat tcattagcaa aatgcatggc accgcgggtg aaagatctgg
    49921 ccaaagattt gcccgcaaga cgttgagcga gtcttcgatc gtgcatccgt cagtcccgat
    49981 gtcgataatc aacagatccg gcggggtgcc gggctgattc cttccgattg cgcggtgcac
    50041 ccaggatacg caagacgtaa acggcaaaac ggcttgccgc agccaggtga agctatcttg
    50101 tgtgggagcg gcaatagcga tgtgctttaa cgcggagtca gtttttacca aaggtgtgta
    50161 tttcaaactg accagcagtc gttcctcttc tgaggccgtg ggcggtaggt tccacgggga
    50221 gccactacct tgccgtaacg catgcagcag cgctcgaccg aagttccacc accgaagcca
    50281 agagatgcga atgacccccg gtagcggtga acccgtccga ggttcgcttt cgcaccagtc
    50341 ggtatagata gccaccgcgc gggtcaaagg cgaatgggcc atgaaggaaa gccagtcttg
    50401 ttcggagaac tggcggggat gatcttccaa caagaggaga acatcgggga aaagaccagc
    50461 gatgaccagc cgttcagcgt cgctaagcgt cggcacgtga aacaatacat cggcgggatc
    50521 gaaggtggac aagaagagac tccattcggg acgacgatca tcgccaacaa tgctgacggc
    50581 gagtctctgt gctgggcgag atgggggtgg tttcatacta cccaagaaac tctcttaggc
    50641 aaataatgcc gcaaaaacga cctaggggat ttcgactact gccggagaac gtctgtcgga
    50701 catgaatacc ttgtgatgtt atccttcacg ggtggcggag acaaggtgca tcgatctccc
    50761 acggctctaa cggaatattc gatataatcg tcgctttgtg gagaggattt agtctgtgta
    50821 ccatttgtgc cgaagcgatt actcagaggt ggatactgtc attgttgttg atggatagag
    50881 aatcggcgtg gccaacgaag gacgaagccc tccccgatga tggcatgtgg ttccggggag
    50941 caggatcata ggcaaaatga agggagtgta cgatgcttgt cctttctcgc cagcgagacg
    51001 agagtatcat catcggggac aacatcgtcg tgacggtggt ggatattcgc ggggataaag
    51061 tgcggttagg gattcaagct ccgccggaaa tcacggtaca tcgtcgcgag gtgtatgaag
    51121 cgattcagcg cgaaaagaag atgaaggaaa gccagcaggc taatccttcg gttcaacctg
    51181 aagtctcagg tcagaaagaa actacgtagc cagtgttcag attgtgacgc ttgtactaaa
    51241 cgaacggcca cgaacgggaa aatccctcac ggttcgtggc cgctttcgtt tttgtgacct
    51301 acagttttat gccgaagcga aaaaaaagag ggattgacga gggtttagca ggttaacgca
    51361 ggttttgtaa agttcccaac ttacccaaac gatatccctc aaaggacgga ttgatccagc
    51421 cagagcgttt tgccgcaagc gtcggcagtg gcccacggaa ggtgatcagt gcgagctgga
    51481 tcgaggccga ggttcggtca aagtcgggta acggttcgat tgtctaaccc gcgcaactat
    51541 aaggacagcg aatcggatcc cgtggttaga ttggtcccga aaagagtttg gcttggtcct
    51601 agctattcag aatgaagggt aacagacatg acccggatta acacgaacat cacctcgttg
    51661 aacgctcaga agacgttggc tcgttcacag gctcaattgc aggaagcctt gacccgattg
    51721 agcaccggcc tgcggatcaa tgtcggtaaa gatgacccgg caggcttgat tgccagtgag
    51781 gccttgcggg cggacatcac cgcggtccaa aaggcgatct ccaacagcga acgtgcaaat
    51841 cagatgatcg ccacggcgga cagcgctttg ggtcagatta gccagcttct caacgatatc
    51901 cggggtctgg tctctgaggc ggccaacaaa ggcgccatga gtgccgagca gatcgccgcg
    51961 aatcagttgc agattgattc ctctctggaa gccattgacc gaatcgccaa ggtcacccaa
    52021 ttccaagggc aacggctcct agatggcagt tttgatttca tcaccacggg tgtggataca
    52081 tccaagatta ccagtttgcg catcgatcag gccaactttg gtgctctgag cagcatttcc
    52141 gtgaatgtgg atattgtctc gcaagcttcg aaagccaaac tgacatacaa ctacggtgct
    52201 ttggccaccg atgtggtcct cgagatcgcc gggaaggacg gggccgaggc cttcagcttt
    52261 gcagcgggta gctccatcga ggatatggcc caggccatta accttgtctc agacgccctc
    52321 ggtgttcgcg ctgttgttca tcaagaagca acggcgggta gtctcacagc ttcgagcttc
    52381 ggctccaata atgacgtgat cattacagcg aacgaggcag ggcaggcggc tggtgacctg
    52441 cggatcaagt acaccaagag ctcttctgcg accactagtg tcgtctatac acccgccagt
    52501 ggcaatacgc ctggtctaat cgaggttcaa ttaggtac
//
LOCUS       DAOSUT010000006        43080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_612_length_43080_cov_9.162069, whole genome shotgun sequence.
ACCESSION   DAOSUT010000006 DAOSUT010000000
VERSION     DAOSUT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 43080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43080
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_612_length_43080_cov_9.162069"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..712
                     /gene="rplU"
                     /locus_tag="PLD05_01125"
     CDS             323..712
                     /gene="rplU"
                     /locus_tag="PLD05_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOP76055.1"
                     /translation="MYAIIQDGGRQYKVQVGEVIDVDYRLEKNKTADPAEFTPKEIRF
                     DRVLAIRDGSKFILGQPTISSAWVVADVIEPILGPKVYIQKFRRRKNYRRRKGHRQLY
                     LRVKIREIHTGQESGSTQAPAVEPAVV"
     gene            909..2174
                     /locus_tag="PLD05_01130"
     CDS             909..2174
                     /locus_tag="PLD05_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76056.1"
                     /translation="MGTKDLIPPHGDLVEPLDLTVLAEAQADFLAHAKKLKKVPVSDA
                     DLSTVYRLGDGGLSPLTGPMDRETYDRVLDEEVIEHNGRLYAWTIPLALPITKALANQ
                     IGPGEEVALVNGRQEIVATLQVTDVFPWDKRKYLTRVYLTERFDHPGGDMVLKADGDK
                     DFLIGGKIQVLPQPKHPRFGKYVLRPKEVRALIREKGWERVVAFQTRNPLHRAHEYAL
                     VYGLETLLRQGYNAGACLNPLIGETKGDDVPADVRMRTYEALIEQRALGDGDSDPNLW
                     GPRNEAVPDRVILVGLDIKMFYAGPKEAVMHAIYRQNFGFTDIIIGRKHADAPYHDGT
                     PIWGDFDAQEIFKRLRGDLKIKPLCVGFAAYYESVGRVDLMENHPGEKPVSISGKEIR
                     AALREGRPVDPRIMRASTARILAEAMANK"
     gene            2200..2940
                     /locus_tag="PLD05_01135"
     CDS             2200..2940
                     /locus_tag="PLD05_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOP76057.1"
                     /translation="MPGGIATYVFGGLSAICMGVSKTGVPGASILAVVLMAEAFRDNA
                     AFSVGALMPVLLVGDVFAVSLYAKDTAWSGLLRLFPYVALGLISGAFLLRYVQGNDLR
                     PVLGGLIVLMFGLEVYRRYRGENHTPHAWWFLLLTGWLAGFSTMVGNAAGPVMTMYLL
                     SQDLPKARFMGTCAVFFFTVNLLKTVPMAWVGMITLDTFRFALFAIPMTFIGLALGRF
                     LFCRMSQQVFDTVALLLAGIGGVWLLIP"
     gene            3112..3516
                     /locus_tag="PLD05_01140"
     CDS             3112..3516
                     /locus_tag="PLD05_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76058.1"
                     /translation="MKLISKNVDVFVAILACVVAMLGCTRQDNAARRLDEGLSQVTTE
                     KVKATPPHDATKPSETPGRMVAPPEHTGPWSIKVSPEPLVPGRSANTGDYRGRWPDYR
                     PPKAILMTAPELPPGIEGPTVDLSVTPSQAGR"
     gene            complement(3568..4542)
                     /locus_tag="PLD05_01145"
     CDS             complement(3568..4542)
                     /locus_tag="PLD05_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP76059.1"
                     /translation="MIKRILVTGGAGFLGSHLCERLVEMGHDVICLDNFFTSQKSNVA
                     HLLDRPNFELIRHDITLPIWLEVDEIYNLACPAAPGHYQYNPIKTMKTSLMGAINVLG
                     MAKRCRAKVLQASTSEVYGDPEIHPQPETYRGSVNPIGPRACYDEGKRAAEALFMDYH
                     RMNRVDIRIVRIFNTYGPRMHPYDGRVVSNFIRQALSGEDLTVYGDGSQTRSFCYRDD
                     MVEGIIRMMDNTEGFIGPVNLGNPEEFTILELAKLVLELTGSRSKIVFKPLPADDPVR
                     RRPDITLARQKLGWEPKTPLREGLKRTIEWFRSIDISHYRPPTPCYEK"
     gene            complement(4660..7548)
                     /locus_tag="PLD05_01150"
     CDS             complement(4660..7548)
                     /locus_tag="PLD05_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76060.1"
                     /translation="MKTLLWFISLKTRAFWLIAVLGLVVTFGLWRLAISYRTAERRED
                     GERITGQLATSLSQRFEKFTNVVDVLRTLRAASREIDDTEWRTALDVLGMSNFPEWET
                     AGITFLSSAPDEKAEASDHVGLPLTFQHLTVPINDPLARKQIEALLSQLVRLLPQAYC
                     DPVVGFVIPLENRFVFLRPVKNLQPICERNAQMSAEIARVFRETPFVLGIFNSQRGLA
                     QTAEKHQPKGTVRLLWTAGNVNSPTELARWSNSRPGSHSASFGCYRSALSWDGPTTIL
                     EYQPDTLRAEETTELLPALAIGFGLLFTSLGCALVAVSTRFRRGVRLLRRRYKTLLDE
                     QEKRYYTIFNTVSEGVLILDDTGHIQECNPAAARILGYPIEEMRGKSLCDFCATPCSK
                     ETDQASCPQALLSGSSETPVVGGVCRRIRRPSGDVRQVMFQTSMLVTGGDKKYVVALH
                     DVTQLNETYERLEQTAQHLQAANVRLRAYSEEVENAARAKIAFLASMSHEIRTPLTAI
                     LGYAQLLAMAKDEEGGISGQVPPQQFSGSAPPYGTKREERSEAEATSPEEAATTISWQ
                     QAVEGIVCNSLHLQDVINNILDYSKLESGRLEIEKLPCEPVPLVRDVLSMLAIKAKGK
                     NLSLGVEIVGSIPRTIQTDPTRLRQILLNLVDNAIKFTSEGRVWVEIELDQTGPERPM
                     LEFRVCDTGMGMSPDQIGRLFQPFRQASEKISRQFGGTGLGLAISRQLARLLGGDVWV
                     ESTPGKGSIFHVRIALGDVSPTDMITAQDIATPGEAVKSQLISSPSTAGPDHLLASAK
                     HRARTIHLKARILLAEDSPDNQRLIRYFLERAGAQVEVASNGQEALEKYHEAVQKGKQ
                     YDLLLMDVEMPVMDGPQAVRQLRAIGCQTPIVALTAHTDPVCVQQFLQSGYTGLIPKP
                     VDRDGLLEAVASILTPRGTKIPTLVQVNEQQWMDHMGASGLVLPVVNQ"
     gene            8034..8609
                     /locus_tag="PLD05_01155"
     CDS             8034..8609
                     /locus_tag="PLD05_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOP76061.1"
                     /translation="MVGTTLELRGSPFLHEIIQEVPGGERIVRCLQCGTCGGSCPNGA
                     DMQYTPRTIFAMIAANQREKVLKANTMWFCVSCYFCVSRCPQKIPITDIMYGLKRIAI
                     RERMYQDTDAPALAKTFTDLLDKYGRSFELGLASRYYLFNRPIAMLKMGPMGLSMFTR
                     GRMSLVPTKIKNIEQLQSIIRKARELGGTQQ"
     gene            8606..9496
                     /locus_tag="PLD05_01160"
     CDS             8606..9496
                     /locus_tag="PLD05_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOP76062.1"
                     /translation="MKYAYYPGCSLECSSAAYDMSVRAVADLLGIELREIDDWNCCGA
                     TEYFSQDELTACAVIARNLALIEPQLDQVVAPCAACYLNLRKVDSLMKEDPALADKIN
                     QCLAAGRLHYDPGRVRVRHLLDLIHTDIGETAIKDKVVQPLTGLRVACYYGCQIVRPY
                     DGFDNPEQPVKLDELMTWVGAEVVDFPVKAHCCGGHMTQISEPQAFELIRRLLHNAAE
                     YRADLILCMCPMCQLNLDAYQNRVNSFFGTNYQIPIIFFTQLLGVAFGLDWKKLGFGK
                     EIVPAEPVLRKKLTAPAATA"
     gene            9611..11635
                     /locus_tag="PLD05_01165"
     CDS             9611..11635
                     /locus_tag="PLD05_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOP76063.1"
                     /translation="MAERVGVYVCHCGSNIAGMVDVEEVARWAGANLKDVVVARDYKF
                     MCSSLGQAMIEEDIKKEGLTRVVVAACSPHLHEKTFRRACENAGLNPYLCQMTNIREH
                     VSWVSKDRAKATEKARALVAAAVERVKHQAPLEPFFVKVNPATLVIGGGIAGLQATLE
                     LADAGYPVYLVERTPSIGGHMAQFDKTFPTLDCSACILTPKMSEAGQHPNVTLFTYAE
                     VEEVSGSVGHFRVKIRKKARYVEEDKCTGCGICVEKCPRKVVDHDFEAGLGYRKAIYT
                     PFPQAVPRIPVIDVESCIWFERQKCRACEKLCPTGAINFDQKDEEITLDVGNIIVATG
                     WKMFDCKKIPQYGYGRLANVFTALEFERMCNAAGPTGGKIVLRDGKTEPQSVAIIHCV
                     GSRDSNHNEYCSGICCMAALKFGHLVLEKTKAEVYSFYIDMRTNQKGYEEFYHRLLEE
                     GMHFVRGKVAEVTDAARRPEEAGKLIIQVEDTLMGKQRRIPVDMVILMGALEPQADAK
                     ELGLKLGISCSMAGWYTERHPKLDPVATMTDGIFVAGVCQGPKDIPASVAQGAAAAAR
                     VMAMITKGDVMIEPVVASIIEEKCSGCRICNNLCPYNAIEYDAEKQVSRVITAMCKGC
                     GTCVAACPAGAITGAHFSNEQVFAEIEGILWDAVHGNGGSGVQEGQPVSA"
     gene            11733..12230
                     /locus_tag="PLD05_01170"
     CDS             11733..12230
                     /locus_tag="PLD05_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOP76064.1"
                     /translation="MANDTFEPKIVGFLCNWCSYRAADLAGTARIKYSPNVRIIRVMC
                     SGRVDPTFVLKALALGADGVMIAGCHPGECHYIEQNYKAMRRYYMLKHMLRALGLEED
                     RIRLVWASAAEGQQLAEHIDKFVEDVRKLGPLNWPANWQENGERLHAVEAIAKEHEES
                     LEVLP"
     gene            12227..13222
                     /locus_tag="PLD05_01175"
     CDS             12227..13222
                     /locus_tag="PLD05_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HOP76065.1"
                     /translation="MSETQGKGKLALYWAASCGGCEIAVLAIDEKILEVAKHFDIVFW
                     PCATDGKVRDVEKMEDGSIDVCLFNGGIRTSEQEYMARLLRRKSKVLVAFGSCATEGC
                     IPGLANLSNREEIFETVYEETPSTENPQHIVPQHETEVPEGKLYLPVFYDTVFTLDQV
                     VPVDYYLPGCPPEAERIWDAIVAILEGKLPPKGSVIGANTTVCDECPRKRTEKKIKKF
                     YRTWEIIPDEETCLLEQGIICCGVATRAGCGARCPRVNSPCIGCYGPNTDVIDYGARL
                     MAALASVIDSNDPQEIEKIIQDGIPDPVGTFYRFSLAHSLLRRKRKLIAAAAGGK"
     gene            13281..14747
                     /locus_tag="PLD05_01180"
     CDS             13281..14747
                     /locus_tag="PLD05_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOP76066.1"
                     /translation="MKRVTIDPITRLEGHGKIEIFLNDQGEVANAYLQIPELRGFERF
                     CVGRPVEEMPNLTARICGVCPEAHHMAATKALDAVFHVDPPATAKKLRELFYSIFFAT
                     DHTTHFYALGGPDFVVGPDAPKAERNILGVINKVGLEIGGQVIKMRHVGHNLIQLMGG
                     RRVHPNWGLPGGVSRGITEEERREIEAAGREAVEFAKFSLQLFDKIVLKNSEYVALIT
                     GDVYVQKTYYMGTVDANNHVNFYDGMIRVVGPDGKEHAKYHPKDYRDYVAERVEPWSY
                     LKFPYLKKVGWKGFVDGPDSGVYQATPLSRLNASDGMATPLAQKEWQRFYEVLGGRPV
                     HHTLATHWARLIELLYATERWVELASDPSITGKDFRVLPKEKPTEGVGCVEAPRGTLT
                     HHYVTDERGLVVKANLIVGTTNNNASICMAVRRAAQKLIKGGQIREGLLNRVEMAFRS
                     YDPCFGCATHSLPGQMPLEVVIRNSSGEVVERLSQYLD"
     gene            14945..16279
                     /locus_tag="PLD05_01185"
     CDS             14945..16279
                     /locus_tag="PLD05_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76067.1"
                     /translation="MSESQPQSPDSFFGPLLAKITCPHCWHRFATPDTLWISEHPDLI
                     GDRLLGPYIPQRFLPNRFTPQGKAIDSRGMETSRLACPNCHLEIPRGAYHLRSLFLSV
                     LGAPASGKSYFLTSMVWRLRRVLPEEFAVTFTDLDAIHNQRLQEYENALFLNEDEDHP
                     VELPKTETTGDLYNTVLFEDSPIQYIKPFLFAVQPMGRHPRVKARSQISRMLVIYDNA
                     GESFLPGGDSAQNPVTRHLALSAAWFFVFDPTQDLRFRKEVSQFSNDPQVRPDGPRGR
                     SVTQRQEAVLHEAWSRVLQHRDSQSSTRAIPLIVVCAKADCWGPLIPGWPLPSPYRRS
                     TQGVAGVDMDVLADVSNKVRELLNRLSPEIVTAAESLTSDVIYLPVSATGCSPETDEN
                     GQIVGFLPKKLNPIWVEVPLIYFMVRWLPTLVFRLRGVYGRRALQPDDIFQQ"
     gene            16376..>17330
                     /locus_tag="PLD05_01190"
     CDS             16376..>17330
                     /locus_tag="PLD05_01190"
                     /inference="COORDINATES: protein motif:HMM:NF040416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76068.1"
                     /translation="MAWELSITSAPRGLHPGRAGFCPVAHTQGMPPQLIERLEKISGY
                     RHLEIGAGNESRNPVVYGHTVLRLGRETFHVLSRICDAGRDYSGRSNRFAYHLVLKPQ
                     ELVPAGPAELLQRPELMLDKWDGQLRVIPTEKEIPKITSEPRPCELWQKVLGDAGWAG
                     VLAQRFLQAPQETIYFVYPLQIDMLKLFQEVIALLPESLRWKVTFSTYYQGLGEVVTC
                     RWRAIPEIASEYAAIMKQQGPQAWDLRSKRGPAPATSEADAAREGRLIELPTPTKVSV
                     PVSEVPTPQPVTVPAPAPREPAFLELANSVDAPPPLWVASKR"
     assembly_gap    17331..17430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17431..18252
                     /locus_tag="PLD05_01195"
     CDS             <17431..18252
                     /locus_tag="PLD05_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76069.1"
                     /translation="EQPVAVGPKEPPQQGKPPGPPAGETAKSDQMTSAPPAEKPPEGK
                     ATGEANRAGGGERSVVSANGPPDQGNAVSEDEFVAAPISELRLKEIVKIFLKKTPTGA
                     PGVKTLQSWESQRLWSRECESTQDFFLLKVVDDNGQAQYYVLSMNSIPVETLASGQEK
                     TIEIPPLVGMTPEVRCVGGWKKGQTTSEFVFSVDGKDRARLKIERKPAKITVKVDEVK
                     QGDGPPLPKDLMAQIGFYASDGPKRVWCPIVDLQIRSGAKVESARENAQPPGGNR"
     gene            18249..21164
                     /locus_tag="PLD05_01200"
     CDS             18249..21164
                     /locus_tag="PLD05_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76070.1"
                     /translation="MTGDQTIVDRIEQALASLDAVSNEELHQLAEEYAEACEQVNRRL
                     QEIHHLIRSGARSEAIRRAEIPPKLLDVVEILDFPDRDAWVDICTLERLPTPPRLLVQ
                     YLSELHQAYEAEQGLSGLLRQHRALAMAQAPLHKRLAILRQLARAEPENPVWQKDIRI
                     LENYWLDKLQREIQERLKAEDLQTLEEILEQLESNEWLEKPSVSLVEQCRSAVASLRG
                     KIFRQEMENIARLVNQALANGDMVRMEEYLRLWDHQAATNPQWVDIQLREQVAEAMSR
                     RHQLEREREEARRRRELLTEFEALLGKSHDLHTLEHHYAELAIRGVAIPEPLEERYRR
                     VTETLRQRAMFRRVVTVAGVAAVVIATLLIVWQVQARMVENRRLSDAVSVVEDLLEKD
                     KLDEAAKYLADFKAAYPTLAQHRHVISLEQKLADAQAAEAKRRETLASLLEKVRASLA
                     QVPDGVSLQKASELARTDAEKQEVMELESQAKVIWANLRQQAEERLRQEIAKLSREYD
                     ALRQRGSVSIDEELQAIARLMNRLTVLSDDNRRQGLSLEGEIRNLREQLSSRFLEVSQ
                     ASEEEKLIRTMCSAAARGPEAYQEAIKKLEPAKSVRIPIGDFQLVEQEFPCWKVIAEW
                     NRVATLWNDPTTSVSDAGAQELFRGAFRLVQVVPPEASAFVQEGQKRILALLDTIARR
                     ELLVADRLTVLVKEWRAPLVADTWQIRLNDGRRYYCLEQPGRTALVLRDLGSLQRNEI
                     TFPRESLVSTGRAPQSVLANQVRDHLADWSASNWETTADTVLGKIIGFCHTVEKDTVE
                     WPLIFHLLYETWSAAKEGDVAWAALWNGYQAKLQPLRDRYPPHYDWIKTAQAAENQYE
                     DIRSVLGSLPTAEDLLSQVKQVRATYRGRRWPTIDLVGLLFEDKTQVWYVRTVGQPAV
                     PDGAVLYVVEQGPEEGQVTLRRIGHWSNSRAVVEPQQSVRFYNGRHVVTLAGEEPGS"
     gene            21161..>21773
                     /locus_tag="PLD05_01205"
     CDS             21161..>21773
                     /locus_tag="PLD05_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOP76071.1"
                     /translation="MSVDSSRQQIHVRFRGRVFGPYSADELRRMVQKGKTAATWEISL
                     DGQNWRPIQELETLLQQSHSVSTWKGPSPSSHGPLGTINDADDDVFVLRGTNAEDAQD
                     IVLPNLQQPEPVTAEVVSSESLWYYTVNDEPEGPIPESHLAAMAATGLIPPDTLVWTV
                     NMSEWRPLRETRLARHLQSARVEQPATVSSLPPAKDFDEEPREA"
     assembly_gap    21774..21873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22005..23504)
                     /locus_tag="PLD05_01210"
     CDS             complement(22005..23504)
                     /locus_tag="PLD05_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76072.1"
                     /translation="MRLASSEKLIILGRLQPVRAFWIIGAVFVFSIITGGTACQRQAS
                     PDHDSPAVKESVVSQEQAHRESPAEPVNPPQYRAQKGPIEFELVQRLAYGTPEEEGTV
                     PPEFAEIIRGLLAARASLEFDTNGRLIGVDLAGERRSGSDAEISLAVRLPHLKRFRVA
                     GFGVTNEGIKQLATCQNLEELGLENTQIDDAGLEYLKALPRLWSLNLRRSVKLTDRAI
                     DTLVGFPRLTHLYLLENQFSAEALMKIAQMDRLRLLDLRGCGAVNSEVLRRLSAMEHL
                     AALRLRGYNIDDVCLQAVGGFSHLTSFTLEESPAGNAGLASLARLPLETVTLFRCTAV
                     NDEGLRIVSQWKKLRSLTLRDMAIRGDFFKELGELPQLRSLSATQTMVSDDALHHLVA
                     CQQLEELALRQTLVTDKGLAVISRLPNLRRLDVAQTMVSDAGLVHLANLKSLEWLDFS
                     GNSGVTDASVKILLKMPQLKEVRLDGTSMTQEAIAQLGPRARTALPVEE"
     gene            24139..25965
                     /locus_tag="PLD05_01215"
     CDS             24139..25965
                     /locus_tag="PLD05_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOP76073.1"
                     /translation="MTRRVVILGTTLIFAAGCSLWFNSKKASSLLDPVETKTPSLVGD
                     LAVPYGLHPIVVEAIGLVTGLNGTGSDPLPSPERQFLLTEMQRRGVQNPNRVLASKNT
                     ALVLVRGYLRPGIQKGENFDVEVRVPSRSETTSLRGGTLLEVPLREMMVLRGAVREGH
                     ILAKAAGPVLVDPSADPEKDAIYATRGRILGGGTADRSRTLGLVLKPDHQSVMNSARI
                     ESAINRRFYVIRQGVQEGVARAKTNEYVEVRLHPRYKNNIARYMAVVRSIPLRDTEPE
                     RLQRLRDLEEELLNPATSEQAALQLEAIGQPAVETLKKGLTHADPAVRFYSAEALAYL
                     DCAEAAGVLGEIARNEPAFRVYALAALSTLNDLETVEILHDLLTSPSAETRYGAFRAL
                     VELNPNDSVIAGEKLNDQFWYHVLRVSGPPMVHVTRNRRAEVVLFGPDITLKSPFALE
                     AGHRIMVTSQQPGKVTVAKFAVNEADQKRFVSNNLDEIIRAVAELGGTYPDVVQMLQQ
                     AKAAGVLEARFEVEAIPETGRKYQGPSEDESPSPDVAPLVKKYEELPGGGDVTTDHAL
                     PGPEFDASAIDTEPTPAEEQNVSETSKKGRSWWPFGGTKTSS"
     gene            26140..30291
                     /locus_tag="PLD05_01220"
     CDS             26140..30291
                     /locus_tag="PLD05_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOP76074.1"
                     /translation="MKSSTRLKENTFGGCGGAAQRILAVAASASRDGLGKTSGKRTPS
                     AAGHSVVGSLDGKARFPNIVAEFTRWPPLGNRLWTYCVVAVGMILSLWFSVAACGEEN
                     GLRISWGGGSARRWAGRISVSQGEILRWQPIGSEADELASLVFRDKGIDIVPTTPRVF
                     QGVDLALDRQGQTLLEIELAAEGTPSQQWSVPIQEIERQPRQMALSGGGQLFIERLPG
                     DELAVSTDRPHLVFSPGETWKFYVTPRGLPFSPGTHVDIRIHIVRSRITDREPRRPLL
                     DSLADLRRTGNTDAPVVRYEITVGKESPIPVEITLPEEEGCYDVVITATPIPSFRLAE
                     SVTRPFGGNASNLERHVQVVVLNTTRRTGEAFSGELTLLGEAVDASNQNFWERLSRVA
                     PYLPRLGFLGRGWRHIGGEGQEAVCELAAASPGEDPAWALYSLPVQEPGRPHLLEIEW
                     PADQRQTMLFSIVEPNAVGAIAPPMVGYGVYSTGEFRRWEQPLGWQVHRVVFWPRTRQ
                     PLLLVANPSGEPALYKKIRVYAGWNRLPEIFPASAFPSERLVAAYFHRPILSTLFGAT
                     DVPGPFQQQGLDDWVTFYQAATRLAEYVTFAGYNAVVLSVAADGSTLYPSERWKPTPR
                     YDSGSFSLTGADPIRKDVLELILRIADREGFSVIPAIDFSVPLPNLEETIRKQGERGR
                     NLRWIGPNGQTLTETQAPYRGMAPYYNILHREVQEELLAVVRELVERYGRHASFYGLG
                     IQISAYGYAQLPGPEWGLDDETIARFSEETGIQLQVPDDNRYQERARLLLGPYSREWL
                     RWRSLRLQTFYSRVTQEVAAVRPDAKVLLLAPTMFVGERWEQRLRPTLTERRDPLALL
                     LETGLTPELATSDLAMGLLRVKRMVEHADFGVRTTEFEIDQIYRSIPMNAQGHMPGVL
                     FYHPPQEIRLPSFDARSPIQPAFTAIIHQPVAWGLENRRRYAQALADGDPTLICDGGW
                     VTPWGGEDMLRPWLAAFRRLPGGPFQDVRFPDQGTSLQPLVVRSLTRQQETYLYFANQ
                     VGFPVILEMTLQAPRGCRMIELSGFRSLPPLESDSQGQCRWKMTFEPYDLVAVKLTTA
                     DITFTSAKVSWSPKVEQRLREQIADLSERAATLSVPPLYAVLENPDFEEPAGDAQPVP
                     LWTALAPAGTMVRVDAAERHGGQQSVYLMSQGPTVSLVSQAFPPPPTGRLTVAVWLKT
                     SHPQRQPPLRCVVTGVHRGQPLSRIAEIGLASPGRTAPVISSDWTPILVEVTDLPMSG
                     LSPLQLRFDLAGQGEVWIDDIQLCHLAFSKAERVELFKLIAPAQAKLENRDIADCLRL
                     LEGFWPEYLARHVPRSEFSVTASDQPREPPESKPSGKPAEKPPDRSAGLLDRVRHILP
                     QALRF"
     gene            30725..31534
                     /locus_tag="PLD05_01225"
     CDS             30725..31534
                     /locus_tag="PLD05_01225"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76075.1"
                     /translation="MTQDEQFPPGFVDIHCHLLPGLDDGPANWDQTLTMARAAVEHGL
                     VWVVATPHQGGRYTENTPARIQSVVQEAKRKLEEHQIPLQLFAGADVRIDETLVERVQ
                     NKEVQPIADGRYLLLELPHDVYFPLQGLLNDLRRLGITGILTHPERNHAIIRRPELLN
                     EILQAGALVQITAGSITGAFGAVVRRFSEELLLSGKVHVVATDAHGRHGRPPHLGDAH
                     RQIARLLGRDTADILCRRNPARIVLGLAPQKLEPVRPIRSLWSRAWRRLAG"
     gene            31556..32326
                     /locus_tag="PLD05_01230"
     CDS             31556..32326
                     /locus_tag="PLD05_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76076.1"
                     /translation="MYPPILLAVMCSSALLGQVAYSPWVETRSVRGLTIYSQFPLQDV
                     ADVQTDLEYLAEDIETALGLRLADRPVCVLLFAGRREYDRFLKERFPDVPYRRALYIQ
                     RSGQAFVLAYRSSELAVDLRHECTHALLHSALPVVPLWLDEGLAEYFEVPRAQRAFGH
                     PHLKTIRLWANLGYVPSLSTLEAIPHMDAMDANKYRWSFAWVHFMLHGPAVARLELVD
                     YLQTLAQYGVPRPLSERLTARLGDPRRAYLDHFKHWTR"
     gene            32561..33994
                     /gene="rimO"
                     /locus_tag="PLD05_01235"
     CDS             32561..33994
                     /gene="rimO"
                     /locus_tag="PLD05_01235"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOP76077.1"
                     /translation="MDDQSNALPIVSTDPEVQASPARSSAKPLGTCSLIALGCPKNLV
                     DGERMLGLLRDAGWQLTTDPQEADVVVVNTCGFLRSAREESFAVIEEMLKLKRKGKIR
                     GVIVTGCLVEKDREALLDRFPEVDQLVNLFARDEITQAAARIQAGLVEQRTVFQPASV
                     VPLDDRRRLRVTPRHLAYLKISEGCDRLCTFCLIPHIRGRHVSKPLESIVEEARELVA
                     EGVRELILVAQDLTYYGLDLAGRPLLAELLRRLRELPGLRWLRLMYLYPMHITDELVE
                     VLREGEPVLPYLDLPLQHISDRILKRMKRRVNRRETEALLAKLRESIPNLVLRTTMIV
                     GFPGETEAEFQELVEFVKTQRFERLGVFEYSREPGTPAARLQDHLPRRVIRARREHLM
                     AVQQEIAFEYAQGQVGRQMTILLDQPYPERRHVFIGRTYADAPEIDSVVYVTGKHLQP
                     GQFVTCEIVAAQGYDLVAAPLDEQPAG"
     gene            34041..35018
                     /locus_tag="PLD05_01240"
     CDS             34041..35018
                     /locus_tag="PLD05_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76078.1"
                     /translation="MSRMTRRDWLNTTALGVGLWSMGALAGRKPVSGEEAARPARRFQ
                     AALVCSAIGVRADQRQAIEYAARFGFEAVEPQPDFLSRLSQEELADLRQEMQEKGLTW
                     SAAGLPVDFRGDIDAFTRSLRRFPQFCETLQRAGVDRVGTWISPSHNERTYLENFRLH
                     AKRLRAVANIAGGYGLRLGLEYVGPKKSWSAGRYPFIHTMREMRELIAEIDYPCVGLT
                     LDSWHWYTAGETVEDLRRLPGHRVVIVHINDAPSGIPVEEQIDSVRELPCATGVIDLK
                     GFLGALVEIGFDGPVYIEPFKKELRQLPPEEALQLTAEAMQKAIALVGN"
     gene            complement(35212..35718)
                     /locus_tag="PLD05_01245"
     CDS             complement(35212..35718)
                     /locus_tag="PLD05_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOP76079.1"
                     /translation="MRTSVFVACVAMVACSSQLWAVESKQSPAPRVIDVALHDGGLLL
                     GQVVTPEGLPVPNAAVTVVSGDQVLGTATTDAQGRFAFRGLKGGVYRLNAANGTGMYR
                     LWAARTAPPGAQDSTLIVSGQDLARGQVLPRVRTWMRNPWIVGGAIATAIAVPVAVAE
                     HDEEPSSP"
     gene            36052..>36426
                     /locus_tag="PLD05_01250"
     CDS             36052..>36426
                     /locus_tag="PLD05_01250"
                     /inference="COORDINATES: protein motif:HMM:NF038001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HOP76080.1"
                     /translation="MGVWEAGVAKRDNHYEAAFESYLRTAGIPYVAVDEARRAVMADG
                     RSLKSLDFLVAPPGPVTWLVDVKGRRFPSGLEHHQYWKNWSTQDDLESLMEWEVLFGQ
                     GFRGLFVFAFHVVGNRLPVSPEW"
     assembly_gap    36427..36526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(36534..37868)
                     /gene="hisS"
                     /locus_tag="PLD05_01255"
     CDS             complement(36534..37868)
                     /gene="hisS"
                     /locus_tag="PLD05_01255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOP76081.1"
                     /translation="MGTLIPARTLKGFRDYLPQMMIPRERIMEIARRVYRSYGFVPID
                     TPALEYLEILAGKGSEETDKQLYRFQDHGGRWVALRFDLTVPAARFTAQHLNELGTPF
                     KRYHIAPVWRGENTQRGRYREFVQCDFDTIGTHSLTSDIETTLVIHDLLRAIGFREFT
                     IRINNRRVLNGLLERLGLSDRAVLILRALDKWGKIGSEGVKRELEEVAGLTAEQAAEV
                     LRLTEIHGDMDEVLSRAERLVQGSAVGEEGVAELREVVRGAVAGGVARECLVVDLAIA
                     RGLDYYTGTIYETFLAALPGIGSVCSGGRYDNLAALFTTQPLPGVGASLGLDRLLAAM
                     EELGMLEAVSTTAPVFIVFFDHQHRDDYLRLAAQLRTAGLAVELYPEPKKLAQQLKYA
                     DRRGHRVAILIGEDEFRKGVCQVKDLKAGTQSEVPLDPQCSALIEHLQRLLG"
     gene            complement(37939..38529)
                     /locus_tag="PLD05_01260"
     CDS             complement(37939..38529)
                     /locus_tag="PLD05_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOP76082.1"
                     /translation="MPCPEKNGWERLRDDMATNGKPIKNYWLTRTSAAVRNGRLRRQD
                     VPPGEVLCFHCPAKCCQYIALPIDRPRTWEGFDTVRWFLLHERTAVFVDDGQWYLLVY
                     LTCKALRSDGRCAIYAHRPKVCRDYGMDICEFEENAVYDQFFETAEQLEEYAEAILGP
                     RSGGSIRSPRPNPLKIVEMAEKAVAEHVTCPDGRAK"
     gene            complement(38670..42317)
                     /locus_tag="PLD05_01265"
     CDS             complement(38670..42317)
                     /locus_tag="PLD05_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOP76083.1"
                     /translation="MNRALGTIWSVLRTSCPLWVVSIVVALAVWHSVGGVTRSPAQEP
                     PLRNLLRLPPPDDEGGQTSSPGASSETPGPLAKPTTEAPTSETVAESIPRITSEEITL
                     RIQRVQADGELPDAVKSQVVAAYERAIQELAAAEKFRNQAEQFRQQREAIQEEVKRLE
                     AELAEVSATPFLPPSEGMTLEEIQAAVAEAEKRLNAARLQRDAIDEEVTKRNAARQTA
                     TQRAEAAQKRLEAAREALAASPDPNQPPALTEAQRILRQTELEAARWELEAIKEELAY
                     YDRADETLLPLRRRVAAQRFRTVETVWNQWQQILAQRRQATAVSRVEQARARIAQLPE
                     PLRALAEEVEKKAGEVADLTTRVNQTHVQLTTVKQQLEELQQQFKATKERMEMVGMTG
                     ALGLWLRAQRNKLPETHTLWQRIRKRQDDIRATQWTLLELSDAHSQMPPLEEELTRWR
                     NRLLPLISEDHPEWETNLREVLAEKYTLLDEEVQLYSRWLNDLVDLDNTERAIVRTVE
                     EYRRYIDEHILWIPNTRPVRLSQLSDDLRLTLGVLRRLVSPTTLWDAGHALQIDLGTW
                     PALWVLAGLVLAVWRLALFQERRHLERLAKKVSQGAYHGFWPTLQVLALTVLGTGWWP
                     AVLAFLAWRLGSPINATTMTVAMSHSFWSTAEFLIVFGTLRRLCLPLGLGEAHFRWPS
                     ELTLFVRRQLRWFIPAGMVLVYFCTVIHAMGDQRWEAGPGRIVFLLMLLLYAVLGHIM
                     LRPKGSLLQAVRAHHPQSWLYRGRTLWRLLAVGVPVGLMALTLAGYSYTAVQLMQRFR
                     GSLMALLLVGVGEELFNRWLIVRKQRIGWERFRQRMASEPQIVGEAKTEVGGVSPSQA
                     ESEPDLAVVGQQTRRLFNTIEVLVVLVALGWIWADILPALGFLRQVRLWRGGPMTAVT
                     TAPSTSSAEPSQAGPIALATPAITLADLLLAVITSVAGLLIAKNIPGLMEMVFSHRLP
                     MEPGLRYALQTITSYCVTGVALIMAAGYLGLRWSQARWLVAALGVGLGFGLQEIFANF
                     VSGLIILFERPIRVGDLVTVGEFSGRVTRIRMRATTILNFERRELIIPNKEFITGRVI
                     NWTLTDPVNRVEVAVRVAYGTEPQRVMDILLETAKRNPHVMTDPAPAVVFEGFGDSSV
                     NFRLFAYVSNYDQRLTAINSLHADVYRRLSEEGITIPFPQRDVHLFYESPMPPPSREQ
                     AQRPDQAPDEK"
BASE COUNT         8849 a        12043 c        12815 g         9073 t
ORIGIN      
        1 cctcacccca accctctccc ggagggagag ggggactttt tgggatagat tcttagtaaa
       61 cgccggaggg gtggagatag cctgacgggg gcgaatcgaa ggggctaaca gccaaccgtg
      121 acccttgaac gttcggcagt tgccgggttt aatgagggtt taccgttctc gcgtggggcg
      181 agtcgatcgg cattgcgcct agcgcggtcg tgtggtgccg gagtagtaga tgcactaatg
      241 gtggcaataa gggcccccaa aaatagggca aatcagcgcg aacgaagttt gagacaattt
      301 tttccaggcg aggattcgac ccatgtatgc gatcattcaa gatggtgggc gacagtacaa
      361 ggtgcaggtg ggcgaggtta tcgacgttga ttatcggtta gaaaagaaca agacggccga
      421 cccggcggag ttcacgccca aggaaatccg ctttgatcgt gtgctggcca tccgcgacgg
      481 ttcgaagttt attcttggcc agcccaccat aagctcggcc tgggttgtgg ccgatgtcat
      541 cgagccgatc cttggtccca aggtttacat ccagaagttt cgccgccgta agaactaccg
      601 tcggcggaag ggccaccgac agctttacct gcgggtcaag attcgggaaa tccacacagg
      661 ccaggaaagc ggttcgaccc aggccccggc cgtcgaaccc gctgtcgtct gaagcccgcc
      721 ttttcaaatc tgcagaaatc gcccaccact ggggcagact ggtgcccgac cttctgggag
      781 gtgcggcctg aagaatgggg attgttgggg tgcccgggcg ggatgattgc gtgactcggg
      841 ataagacggg gtgattggga cgagattgac ggttttttct gagtaatatt tcagtcacaa
      901 ttgcgaatat gggaacgaaa gacttaattc ctcctcatgg tgacttggtt gagccgctcg
      961 atctgaccgt cctggcggag gcccaggccg atttcctggc ccacgccaag aagctcaaga
     1021 aggtgcccgt ttctgatgcc gatttgtcca cggtttatcg cctgggcgat gggggattga
     1081 gtcctttgac cggtcccatg gaccgcgaga cgtacgatcg ggtgttagac gaggaggtga
     1141 tcgaacacaa tgggcgattg tatgcctgga cgatcccctt ggcacttccc attacaaagg
     1201 ccttggccaa tcaaattgga cctggtgagg aggtggcgct tgttaacgga aggcaagaga
     1261 ttgtcgccac gttgcaagtg accgatgtct tcccctggga taagcggaag tatttgacgc
     1321 gggtctatct cacggaacgg tttgaccatc ccggcggtga tatggtcctt aaggctgatg
     1381 gggataagga ttttctcatc ggtggaaaaa tccaggtctt gccgcagccg aagcatccgc
     1441 ggtttgggaa atatgttctc cgtccgaaag aggtgcgggc gctcattcgg gaaaagggat
     1501 gggagcgggt agtggcgttt caaacgagaa accctctcca ccgggctcac gagtatgctc
     1561 tggtttatgg cctagagacg ttgcttcggc aaggttacaa cgccggagcg tgcttaaacc
     1621 ccttaattgg cgagacaaag ggcgatgatg ttccagcgga tgtccgcatg agaacgtacg
     1681 aggccctgat tgagcagagg gccttgggag acggcgacag cgatcccaac ttgtggggcc
     1741 cccgaaacga agcggtcccg gatcgtgtca ttctcgttgg cttggacatc aagatgtttt
     1801 acgccgggcc caaagaggcc gtcatgcacg cgatctaccg gcagaacttt gggtttacgg
     1861 acatcattat tgggcgaaag catgcggacg ccccgtatca tgatggcacg ccgatttggg
     1921 gagacttcga cgcccaggag attttcaagc ggcttcgcgg cgatctaaag atcaaacccc
     1981 tttgcgtggg ttttgccgcc tactacgagt cagtggggcg ggttgatcta atggaaaacc
     2041 atcccggcga aaagcccgtc tcgatttcgg gtaaagaaat ccgggcggca ttgcgggaag
     2101 gtcggccggt cgatccacga atcatgcggg cgagtacggc gcggattttg gccgaggcca
     2161 tggccaacaa atgacatcgc tcaccgggag tcccatttca tgccgggagg aattgctaca
     2221 tacgtcttcg gggggctttc ggctatctgt atgggggttt ccaagacggg cgttcccgga
     2281 gcctcgatct tggctgtcgt tctcatggcg gaggcatttc gggacaacgc cgctttctcg
     2341 gtgggtgctt tgatgcccgt gcttttggtg ggcgatgtct ttgccgtgag cctttatgcc
     2401 aaagataccg cgtggagtgg tctcctgcgg ttatttcctt atgtcgcgtt gggactgatt
     2461 tctggcgcat tccttttgcg ctatgttcag ggaaacgacc tgcggccggt gctgggcgga
     2521 ttgatcgtcc tcatgtttgg tctggaggtc tatcgccggt atcggggtga aaatcacacg
     2581 ccacatgcgt ggtggttttt gcttctcacg ggttggttgg cgggcttttc cacgatggtg
     2641 ggcaacgctg ccgggccggt gatgacgatg tacctcctca gccaggatct gcccaaggcc
     2701 cggttcatgg gcacctgtgc cgtgtttttc ttcaccgtga atctcttgaa gactgtcccg
     2761 atggcctggg tggggatgat caccctggat acatttcgct ttgccctgtt tgcgattccc
     2821 atgacgttca ttggccttgc cttgggaagg ttcctgttct gtcgcatgtc gcagcaggtg
     2881 tttgatacag tggccctatt gctagcggga atcggtggcg tgtggcttct cattccctga
     2941 aggggcgtgt cattttcgct attgcagttg gccggttttg ctttgccatg tttgccaaag
     3001 gtgtaagcac tccgttgctg cgttccgagg aaggacagag ctacaatgga cgggatcggg
     3061 ggatcactgc tgtgtcgttt ttgccagact taatgtgaat cagagcaaat catgaagcta
     3121 atcagtaaaa acgttgatgt gtttgtggcg attttggcct gtgttgtagc gatgctggga
     3181 tgcacacggc aggataatgc tgctcggcgg cttgatgagg gactctctca ggtgacgaca
     3241 gagaaggtca aggcgacgcc tccccacgat gcgaccaagc cgagtgagac gcctggccga
     3301 atggtggctc cccccgagca cacaggcccc tggtctatta aggtatctcc tgagccgctc
     3361 gtgccggggc gatcggccaa cacgggtgat tatcgtggcc gttggcccga ctatcggccg
     3421 ccaaaggcaa tcttgatgac agcaccagag cttccgccgg ggattgaggg gccgaccgtc
     3481 gatctgtcgg taacgccctc tcaagcaggt cgctgatgat tgtggcaggg ctgccacctg
     3541 cggaggtcct gcagtgataa gcctgtccta cttctcatag cacggagtgg gcggccgata
     3601 gtggctgatg tcgatcgacc ggaaccactc gatggtccgt tttaaacctt cgcggagggg
     3661 cgttttgggt tcccagccca gtttttggcg ggccagggtg atgtcgggcc gacgtcgaac
     3721 cgggtcgtcc gcgggtaggg gcttaaagac gattttggag cgcgatccgg ttaattccag
     3781 gacgagtttg gccaactcga ggatggtaaa ctcctcgggg ttgcctaggt tcacaggccc
     3841 gatgaagccc tcggtgttat ccatcatgcg gatgatgcct tcgaccatgt cgtcccgata
     3901 gcagaacgag cgggtttggg agccgtcgcc atagacggtg aggtcttcgc cggagagggc
     3961 ttgacggatg aagttggaaa cgacgcggcc atcatagggg tgcattcgtg ggccatatgt
     4021 gttgaaaatc cgcacaatgc ggatatcgac ccgattcatg cgatggtaat ccataaagag
     4081 ggcctccgct gcccgctttc cctcgtcgta gcaggcgcgg ggaccaatgg gattgacgga
     4141 tccgcggtag gtttccggct ggggatggat ttcgggatcg ccatagacct cgctggttga
     4201 ggcttgtaac accttggccc gacaccgctt ggccatgccc aggacgttga tcgcccccat
     4261 cagcgaggtc ttcatcgtct taatggggtt gtattggtaa tgtccaggcg ctgctgggca
     4321 cgccaaattg tagatttcgt ccacctccaa ccagatcggc agcgtgatgt cgtgacgaat
     4381 caactcaaag ttgggtcggt ccagtaggtg ggcgacattc gacttttgac tggtaaaaaa
     4441 gttgtccaga caaataacgt cgtgccccat ctcaacgagg cgttcacaaa ggtgggaacc
     4501 gagaaaaccg gcgccaccag ttaccaagat tcgtttgatc atcgccatcg cgtcatcctc
     4561 tcgccgtgtt ctcaaaaggc ctttttgcta tcttgggcgg agtggtttct tctgtaaagc
     4621 cgagccgaga taacagaagt ggaatccttt atcccgctgt cattgattga caacgggtag
     4681 gacgagcccc gacgcaccca tgtggtccat ccattgttgc tcgttgacct ggaccaatgt
     4741 gggaatcttt gtgcctctcg gtgtgaggat actcgccacg gcctccagca ggccatcccg
     4801 atccaccggt tttgggatca gccctgtgta gccggactgg agaaactgct ggacgcacac
     4861 cgggtctgtg tgggcggtca gggcgacaat tggggtttga cagccgatcg cgcgaagctg
     4921 ccgaaccgct tgggggccat ccataaccgg catttctaca tccataagga gcaaatcgta
     4981 ttgtttgcct ttctgcaccg cctcgtggta tttttccagc gcttcttgtc cattactggc
     5041 aacttccact tgggccccgg cacgttcgag gaaatagcgg atgagccgct gattgtcggg
     5101 agaatcctcc gccaaaagga tgcgagcctt caagtggatg gttcgtgccc gatgctttgc
     5161 ggacgccaac aagtggtccg gtccggcggt ggacggggac gagatgagct gtgacttgac
     5221 ggcttctccc ggcgttgcga tgtcctgggc ggtgatcata tcggtcgggg agacgtctcc
     5281 gagggcaatc cggacgtgaa agatcgagcc ctttcccggg gtgctttcca cccagacgtc
     5341 gccgcccaac aagcgagcca attgccggct aatggcaagc cccaggcccg ttccaccgaa
     5401 ttgccgagag attttctcgc tcgcttggcg aaacggctga aagagccgtc cgatttgatc
     5461 gggagacatt cccatgcctg tgtcacagac gcgaaactca agcatgggtc gctctggacc
     5521 tgtttggtca agctcgattt caacccagac ccgcccttcg ctcgtgaatt tgatggcatt
     5581 gtccacaagg ttaagcagga tctgtctgag gcgggtcgga tctgtctgga tggtccgtgg
     5641 gatgctgccc acgatttcca cacccagtga aagattcttg cccttggcct tgatggccaa
     5701 catggagagg acgtccctta caaggggaac gggttcgcac ggcagttttt cgatttccaa
     5761 tcgtccgctc tccaatttgg agtagtccaa aatgttgttg atgacgtctt gcaaatgcag
     5821 gctgttgcag acaattccct ccacggcttg ctgccaagag atcgttgtcg cggcctcctc
     5881 cggagaagtc gcctcggctt cgctgcgttc ttcccgcttt gtgccatacg gcggcgccga
     5941 gccggaaaac tgttgtggcg gcacttggcc cgagatgcca ccttcctcgt cttttgccat
     6001 agccaggagt tgggcgtaac cgaggatggc ggtcaagggt gttcggattt cgtggctcat
     6061 gctggccaaa aaggcgatct tggctcgcgc ggcattttcc acttcttccg agtaggcccg
     6121 cagtcgaaca ttggctgcct gaaggtgttg ggctgtttgt tccaggcgtt cataggtctc
     6181 gttcaactgc gtgacatcgt gaagggccac cacatacttc ttatcgcctc ccgtgacaag
     6241 catagatgtt tgaaacatga cttggcgaac atcgccggat ggtctgcgga tgcggcggca
     6301 tacgcctcct acgaccgggg tttcactgct accggataac agggcctgcg gacaacttgc
     6361 ttgatccgtt tcttttgaac aaggtgtggc acaaaagtca caaagggact ttccccgcat
     6421 ctcttcgata ggatatccga gaatgcgggc ggcggccgga ttgcattcct gaatatggcc
     6481 cgtgtcgtcc aagataagga ccccttcgct caccgtattg aagatggtgt agtaccgctt
     6541 ttcttgttca tctaagaggg ttttatatct ccgccggaga aggcgcacgc cccgccgaaa
     6601 tcttgtggac acggcaacca gagcgcagcc cagggacgta aaaagcagac caaaaccgat
     6661 ggcaagggcg ggtaaaagct cggttgtttc ttccgctcgc agtgtgtcag gctggtattc
     6721 caggatggta gtgggcccgt cccaggaaag agcactccga tagcagccga aggatgctga
     6781 atgcgacccg ggtcgagagt ttgaccatcg tgccaattct gtcggtgagt ttacgttgcc
     6841 tgctgtccac aacagccgga ccgtcccctt tggctggtgc ttctccgccg tttgcgcgag
     6901 acccctctgg ctgttaaaga tgcccagcac gaaaggcgtc tcgcggaaca cgcgggcgat
     6961 ttcggcagac atctgggcgt tccgctcgca gatcggttgg aggttcttaa ccggtctcaa
     7021 gaagacgaag cggttttcga ggggaatcac aaaacctacc acaggatcgc agtaagcttg
     7081 cgggaggagc cgtaccagtt gcgaaaggag ggcttcaatc tgcttcctgg caagcgggtc
     7141 gttgattgga accgtcaggt gctggaatgt cagcggcagg ccgacgtgat ccgacgcctc
     7201 ggccttttcg tcaggggctg aagataggaa agtgattccg gcggtttccc actcggggaa
     7261 gttcgacatg ccgagaacgt ccaaggcggt ccgccattcg gtgtcgtcaa tttcgcgact
     7321 ggcagcccgc aaggtacgga ggacatccac gacgttcgtg aacttttcaa accgttgcga
     7381 aagcgacgtg gcgagttgtc ccgtgatccg ctcgccatct tcgcgccgct cggcggtgcg
     7441 gtaggagatc gcgagcctcc agaggccgaa ggttacgacc aaacccagga ccgcaatcag
     7501 ccaaaacgct cgtgtcttca aagaaatgaa ccaaagcagc gttttcatcg aaataaatta
     7561 cgtgcgctct ccaaaggacc catgataagc ctgcgggagg ggttttccgc caggacgaag
     7621 aaaaaagaat atccccgcgg cctttaggaa aatataggtc ccatgagagg agacaaccca
     7681 tcctgaagct gctttctggc ccttgccaac gggggggaag tcccgaacaa ccgtgatatt
     7741 tcgcataatt gcgggatagg gccacagttc aacggggcgc ggtgccgaat ggaatgagaa
     7801 gtcgttttta tgtcgcggaa aatcgcggga gaccgcggat tttcgttttt tgtgcgaaag
     7861 ggcagccagc cttcctgaga tggcggattc gccataagta tagtttaata cgtaagatcg
     7921 gtaaggttgg gcagcggcaa gcggtaatcg gtattcgatt tgcgtagcta ggcacgtacg
     7981 gagcgtgttt ataagtttgc aacagataaa tatgtcttat ttggggtgac tcaatggttg
     8041 gaacaacatt ggaactgcgg ggttcgccct ttcttcatga gatcattcag gaagtaccgg
     8101 ggggtgagcg gatcgtccgc tgcctgcaat gtggcacctg cggggggagt tgtcccaacg
     8161 gtgcagacat gcaatacacg ccccgcacca tctttgccat gattgctgcc aaccagcggg
     8221 agaaggtcct caaggcgaac acgatgtggt tctgtgtctc ctgctacttc tgtgtcagtc
     8281 ggtgtccgca gaagatcccg atcacggaca tcatgtatgg gctgaagcgg atcgctatcc
     8341 gcgaacgcat gtaccaggac acagatgccc ccgcgctggc caagacgttc accgacctgc
     8401 tggacaaata cggtcggagt ttcgagctag ggttggcaag ccgatactat ctgttcaatc
     8461 gaccaattgc gatgctcaaa atggggccga tggggctctc catgtttacc cggggacgca
     8521 tgtcccttgt gccgaccaag attaagaata tcgagcagct ccagtccatc attcgtaaag
     8581 ctcgtgagct gggaggtacc caacagtgaa atatgcctat taccccggtt gttcgttaga
     8641 gtgttccagt gctgcgtacg acatgtcggt gcgtgccgtg gccgatcttt tggggattga
     8701 gctgcgggaa atcgacgact ggaattgttg cggcgcaaca gagtacttta gccaggacga
     8761 gttgacggca tgcgccgtca tagctagaaa tctcgccctt attgagccgc agctcgacca
     8821 ggtggtggcc ccgtgtgcgg cctgctactt gaacttgagg aaggtcgatt ccttgatgaa
     8881 ggaagacccc gccctggccg acaagatcaa tcagtgcttg gcggcgggac gtctgcacta
     8941 tgatcccggt cgggtccgcg tccgccatct tttggatttg atccacacgg atattggcga
     9001 gacggccatc aaagataaag tcgttcagcc tctcacgggt ttgcgggtgg cctgttacta
     9061 cggatgccag attgttcgcc cctatgacgg gttcgacaac cccgagcagc ccgtcaaatt
     9121 ggacgagttg atgacctggg taggagcgga agtcgtcgat tttccggtca aggctcactg
     9181 ctgtggcggt catatgacac aaatcagcga gccgcaggcc tttgaactga ttcgccgact
     9241 gctccacaat gcggcggagt atcgggcgga cttgatcctg tgcatgtgcc cgatgtgcca
     9301 gcttaacctg gacgcttacc aaaaccgcgt caacagtttc tttggcacaa attaccaaat
     9361 tcccatcatt ttcttcacgc agttattggg agtggctttt ggtttggatt ggaagaagct
     9421 cggttttggc aaagagattg ttccggcgga gccagtcctg cggaagaaac tgacggcccc
     9481 ggcggcgacg gcgtgaggtg tcgagggcgg cccagcgggg ccgtggtcgg tcagccgctg
     9541 cctttggcta tttgacttga ggtgatccat cgagttggga ccagacagat tatttgagga
     9601 gagagatccc atggccgaac gcgtgggcgt ttatgtttgc cactgtggaa gtaatatcgc
     9661 ggggatggtt gatgtggaag aggtggcccg ttgggccggg gcaaatctta aagacgtcgt
     9721 cgttgcccgc gattacaagt tcatgtgctc gtcgcttggt caggccatga ttgaggagga
     9781 catcaagaag gaaggcctga cgcgcgtggt tgtggcggct tgttccccgc atttgcacga
     9841 gaagacgttc cgtcgggcat gcgagaatgc ggggctcaat ccgtatctgt gccaaatgac
     9901 gaacatccgc gagcacgtct cctgggtttc caaagatcgg gccaaagcca cggaaaaggc
     9961 ccgggccttg gtcgccgccg ccgtggaacg cgtcaaacac caagcaccgc tggaaccatt
    10021 ctttgtgaag gttaacccgg ccacgctcgt gatcggtggc ggcatcgccg gtttgcaggc
    10081 cacattggaa ttggccgacg cgggctatcc agtttatctc gtcgaacgaa cacccagcat
    10141 tggcggccac atggcgcagt tcgacaagac ctttcccact ttggactgct cggcatgtat
    10201 cttgacgccg aagatgtccg aggccggaca gcatcccaac gtgacgttgt tcacctatgc
    10261 tgaggtggag gaggtgagtg gctcggtggg ccatttccgc gtcaaaatcc gcaagaaagc
    10321 ccgctatgtg gaagaggaca aatgcacggg ctgcgggatt tgcgtggaaa aatgcccacg
    10381 aaaggtggtg gatcacgatt ttgaggccgg cttaggatac cgcaaggcga tctacacgcc
    10441 gttcccccaa gccgttccga gaatccccgt cattgacgtg gaatcgtgta tttggtttga
    10501 aaggcagaaa tgccgggcct gcgaaaagct ctgtcccacg ggcgccatca acttcgacca
    10561 aaaagacgaa gagatcacgc tcgatgtggg taatattatc gtggcaaccg gctggaagat
    10621 gttcgactgc aagaagattc cgcagtatgg ttacggacgt ctggcgaacg tcttcacggc
    10681 cttggaattc gagcggatgt gtaatgcggc cgggccgacg ggcgggaaga tcgtcttgcg
    10741 agacggcaag accgagccac aatcggtggc cattatccac tgtgtgggca gccgggattc
    10801 caaccataac gagtattgct ccggcatctg ctgcatggcg gccctgaagt ttggacacct
    10861 ggtcctggag aagacgaagg ccgaggtgta ttctttttac atcgacatgc ggaccaacca
    10921 gaaaggttac gaagagttct accatcgcct tttagaggaa gggatgcact ttgtccgtgg
    10981 taaagtggct gaggtcaccg atgcagcccg gcgtcccgag gaggccggaa agctcatcat
    11041 ccaggtcgaa gacaccttga tgggcaagca gcggcgaatc cccgtggata tggtaatcct
    11101 tatgggggcc ttggagccgc aggccgatgc caaggagctg ggactgaaac tcggcatctc
    11161 gtgcagcatg gcgggatggt acacagaacg gcatcccaag ttggacccgg tggccaccat
    11221 gacggacggc atttttgtgg ccggtgtctg ccagggcccc aaggatattc cggcctccgt
    11281 ggcccaaggg gctgctgccg ccgcccgggt gatggccatg atcaccaagg gcgatgtcat
    11341 gatcgagccg gtggtggcct cgattatcga agaaaagtgc tcgggatgcc ggatctgcaa
    11401 caatctttgt ccctacaacg ccatcgaata cgacgcggaa aaacaggtca gccgggttat
    11461 cacggcgatg tgcaaaggtt gcgggacatg tgtcgccgcc tgcccggcag gtgccatcac
    11521 tggtgctcac tttagcaacg aacaagtttt cgcggaaatc gaagggattt tgtgggatgc
    11581 cgttcatggg aacggtggat cgggcgtcca ggagggccag cccgtttccg catgacggtc
    11641 atccacggta tgtcactatt tggggaacgt tgatcagaag accaactgca actgcggtgc
    11701 tttgagcggt tgattgcgaa taggactgag ctatggcaaa tgacacgttt gaacccaaaa
    11761 ttgtcgggtt tctttgcaac tggtgctcgt accgagcagc ggacctggcc ggcacagccc
    11821 gcatcaagta ttcgccgaac gtgcggatta tccgggtgat gtgcagcggt cgggttgatc
    11881 ccacctttgt cctgaaggca cttgccctag gggccgatgg cgtgatgatc gctggttgcc
    11941 acccgggaga atgccactat atcgaacaga attacaaggc gatgcggcgg tattacatgc
    12001 tcaagcacat gctgcgggca cttggtttgg aggaagaccg gattcgcctg gtctgggcgt
    12061 cggccgccga gggacagcaa ttggctgagc atatcgacaa gttcgtggaa gacgttcgca
    12121 agctcggtcc cctcaactgg ccggccaatt ggcaggagaa cggtgaacga ctgcacgccg
    12181 tcgaggccat cgccaaggaa catgaagaat cgctggaggt ccttccatga gtgagacaca
    12241 aggcaaagga aaactggcgc tgtattgggc tgcatcgtgc ggcggctgcg agatcgctgt
    12301 cctggcgatc gacgaaaaga ttctggaagt tgcgaagcac ttcgatatcg tgttctggcc
    12361 ttgtgcgaca gacggcaagg tccgcgatgt ggaaaagatg gaggatgggt ccattgacgt
    12421 gtgcctcttc aatggaggca ttcggaccag tgaacaagag tacatggccc gcttgctgcg
    12481 gcggaagtcc aaggtcctgg tggcctttgg ctcgtgcgcc acggaaggtt gtatcccggg
    12541 gctggccaat ctcagtaatc gggaagagat tttcgaaacg gtgtacgaag aaacgccttc
    12601 caccgagaat ccgcagcaca ttgttcccca gcatgagacg gaagtcccgg aagggaagct
    12661 ctatttgccg gtcttctatg acacagtctt tacgttggat caggttgtgc cggtggatta
    12721 ctacctcccc ggttgcccac cagaggcgga acggatttgg gacgcgattg tggccatctt
    12781 ggaaggaaag ctcccgccga aggggtccgt gattggggcc aacaccaccg tgtgcgatga
    12841 gtgtccccgg aagcggacgg aaaagaagat caagaagttc taccgaacgt gggaaatcat
    12901 tccggacgag gaaacgtgcc tcttggagca ggggattatc tgttgtggtg ttgccacgcg
    12961 ggcgggttgc ggggcacgct gtccacgtgt taattcgccg tgcattgggt gctatggacc
    13021 gaacacggat gtcattgatt acggcgcacg tctgatggcg gccttggcat cggttattga
    13081 ttctaacgac ccccaggaga tcgagaagat cattcaagat ggtatccctg atcccgtggg
    13141 gactttctac cgcttcagtt tggcgcatag tttgttacgg cgtaaacgaa agctcatcgc
    13201 cgccgccgcg ggaggcaagt aacgtcaatt tgatcaataa cactcggtac gatccttatt
    13261 gaaggcgact tgagggaact atgaaacgcg tgacgatcga tcccatcacc cggttggaag
    13321 gtcacggaaa gatcgagata tttctgaatg accaaggcga ggtggccaac gcgtatttgc
    13381 agattccgga attgcgggga tttgaacggt tttgcgtagg tcggccagtg gaagagatgc
    13441 ccaacctgac ggcccggatt tgcggcgtct gtccggaagc ccaccacatg gccgccacca
    13501 aggccctgga tgcggtgttc cacgttgatc ctccggccac ggccaagaaa cttcgcgagt
    13561 tgttttacag catctttttc gccaccgacc acacgaccca cttttacgct ttgggtggtc
    13621 ccgatttcgt tgttgggcca gatgccccca aggccgaacg aaacatcctt ggcgtgatta
    13681 acaaggtagg cttggaaatc ggtggtcagg tcatcaagat gcggcatgtt ggccataacc
    13741 tcatccagtt gatgggcggt cgccgcgttc atcccaactg gggccttccc ggtggtgtca
    13801 gccgagggat cacagaagag gaacgacgtg agatcgaggc cgccggacgt gaagccgtcg
    13861 agttcgccaa gttcagcctc cagctcttcg ataagatcgt gctgaaaaat agtgagtacg
    13921 tcgccctcat cacgggcgat gtttatgttc aaaagaccta ctacatgggc accgtagatg
    13981 ccaataacca cgtgaatttt tatgacggca tgattcgcgt ggtcgggccg gacgggaagg
    14041 aacacgccaa gtatcatccc aaagattatc gagactatgt ggccgagcgc gtcgagcctt
    14101 ggagctattt gaagttcccg tacctcaaga aggtcggttg gaagggcttc gtcgatggtc
    14161 cagattccgg tgtctatcag gccacaccgc tgtcacggct taacgcttcc gatggcatgg
    14221 ccactcctct ggctcagaag gaatggcagc ggttttacga agtgcttggt ggtcgtccgg
    14281 tgcatcatac gctggccacg cactgggccc gcttgatcga attgctgtac gccaccgagc
    14341 gttgggtgga attggcctcc gatccaagca tcacggggaa ggatttccgc gtactgccaa
    14401 aagaaaagcc caccgaaggc gtcgggtgtg tggaggcccc tcgcggtacc cttacccatc
    14461 actatgtgac cgatgagcgt ggcctggtgg tcaaagcaaa cctgattgtc gggacgacca
    14521 acaacaatgc ctccatctgc atggcagtgc gccgggcggc ccaaaagctc atcaagggtg
    14581 gccagatccg cgaaggtttg ctcaaccgcg tggagatggc cttccgttcc tatgacccat
    14641 gcttcggctg tgcaacacat agcctgcccg gccaaatgcc gctggaagtc gtcatccgca
    14701 attcttccgg cgaggtggtg gagcgtctaa gccagtatct ggactgacgg ggtatcaccc
    14761 ggcatcatcc caccgctcca tgacggagtg tcctttaacc tgcgagggga taaatgatct
    14821 cctcgctttt tttctttttt ggggggcaca tctggaaatg gtgatacggt cccggagatt
    14881 tggtcattga ccgccctcga tggggaacgg acactaataa gaggggcatt cccggaggtg
    14941 gtacatgtcc gaatcgcaac cgcaaagtcc tgactccttt tttgggccgc tcctcgcaaa
    15001 gatcacgtgt ccgcattgct ggcaccgctt tgccactcct gacacgttat ggatttccga
    15061 acatccagat ttgattgggg atcgcctgct tggcccttac atcccacaac ggtttcttcc
    15121 caatcgtttc acaccccagg gaaaggccat tgacagccgg gggatggaga cctcgcgact
    15181 tgcttgtccc aactgccatt tggaaatccc ccgaggggcg tatcacctgc ggtcgctgtt
    15241 tttgtcggtc ttgggggcac cagcttcggg caaatcctat ttcctcacca gtatggtgtg
    15301 gcgcctgcgg agagtgttgc ctgaagagtt cgccgtgacc tttaccgatc tggatgccat
    15361 tcataatcaa cggcttcagg aatatgaaaa cgccctgttt ttgaacgagg atgaagatca
    15421 cccggtagaa ctgcccaaga cggaaacaac gggcgacttg tacaatacgg tcctctttga
    15481 ggattcgccg atccaatata tcaagccgtt tctctttgcc gtgcaaccca tgggaaggca
    15541 tccgcgagtg aaggcccggt cgcagatcag ccgcatgctg gtcatttacg acaatgcggg
    15601 cgagagtttc cttcccggcg gggactccgc ccaaaacccc gtgacgcgac atcttgccct
    15661 ttccgccgcg tggttctttg tctttgatcc cacccaggat ttacgttttc gcaaggaagt
    15721 gagccagttt tcaaatgatc cccaggttcg gccggatggg ccgcggggac gcagtgtgac
    15781 tcagcgacaa gaggcggtcc tccacgaagc gtggagccgt gtcctgcaac atagggatag
    15841 ccaatccagt acccgcgcca tccccttgat cgtcgtctgt gccaaggccg actgttgggg
    15901 accacttatt cccggctggc cgttgccttc gccgtaccga cggagcaccc agggggtggc
    15961 cggtgtcgat atggatgtac tggctgatgt gtccaataag gtgcgggaac ttcttaaccg
    16021 tctttcaccg gaaattgtca ccgccgcgga gtcgctgaca tccgatgtca tttatctgcc
    16081 cgtcagtgcc acagggtgtt cacccgaaac ggatgagaac ggccagatcg tggggttttt
    16141 gcccaagaag ctcaatccca tctgggtgga agttcccctg atctacttta tggttcgctg
    16201 gctgcccacg cttgtatttc gtttgcgagg cgtttacgga cgtcgggctt tgcagcctga
    16261 cgacattttt cagcagtgag tgtcgtgagc caggagtgac atgagaaaga ggcaatagag
    16321 caccgatctc tgaaaggcca cttaagcgca gaagcccctg aggaggttca ttatcatggc
    16381 gtgggaactg agcatcacct cagcgccgcg tggtttgcac ccgggccgcg ccggattttg
    16441 tcctgtggcg catacgcagg ggatgccacc tcaactgatc gaacggctgg agaagatcag
    16501 cggctaccgt cacctggaga tcggtgcggg caatgaatcg cgcaatcctg tcgtttatgg
    16561 tcacacggtc ctccgtcttg gccgcgagac gtttcatgtc ctttcgcgga tttgcgatgc
    16621 gggacgcgat tattcgggac gttccaaccg ctttgcctac cacctggttc tcaaaccgca
    16681 ggagttggtc cccgccggcc cggccgaact ccttcagagg ccggaactga tgctcgacaa
    16741 gtgggacggc cagctccgtg tgatccccac ggagaaggaa atcccaaaaa tcacctctga
    16801 accccgccct tgcgaactgt ggcaaaaggt tttgggcgat gccggctggg cgggggtcct
    16861 cgcccaaagg tttctccagg cccctcaaga gacgatctat ttcgtgtatc cgctccagat
    16921 cgatatgctg aaactgtttc aggaagttat tgccctgctg ccggaatcgc tccgctggaa
    16981 agtgacgttt tcgacatatt accaggggct gggagaggtc gtcacctgcc gatggcgggc
    17041 aattcccgag atcgcctccg agtacgcggc catcatgaaa caacagggtc ctcaggcgtg
    17101 ggacctgcgg agcaaacggg gacccgcccc ggcgactagc gaagccgatg cggcccgcga
    17161 aggtcgcttg atcgagttgc caacgccgac caaggtgtcg gtccccgtgt cggaggtacc
    17221 aacgccgcag ccggtaacgg tgcccgcacc ggcgcctcga gagcctgctt tccttgaatt
    17281 ggcgaacagt gtcgatgccc cgccaccact ttgggtggcc tctaagcgag nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaacagccgg ttgccgtcgg tcccaaggaa
    17461 ccaccccaac aaggcaagcc gccagggccg ccagcaggtg agacagccaa atctgatcaa
    17521 atgacttccg cacccccggc ggagaagcct cccgagggaa aggctaccgg ggaggccaac
    17581 cgcgccggtg gcggcgagcg atcggttgtt tctgccaatg ggccacctga ccagggtaac
    17641 gcagtttcag aggatgaatt cgtggcggct ccaatcagcg agttgcgtct gaaagaaatc
    17701 gttaaaatct ttttaaaaaa gacgccaaca ggcgccccgg gcgtgaaaac tctgcaatct
    17761 tgggagagcc agcgtttatg gagtcgggaa tgtgagtcca cccaggattt ctttctcctt
    17821 aaagtcgtcg atgacaatgg ccaggcccag tactatgttc tgtccatgaa ttcgattccc
    17881 gtagaaacct tggcatcggg ccaggaaaaa acgatagaga ttcctccttt ggtgggaatg
    17941 acacccgagg tgcgatgcgt tggcggttgg aagaaaggtc agacgacttc tgagttcgtc
    18001 ttttccgtcg atggtaagga tcgggcgaga ctcaagatcg agcgcaaacc ggcgaaaata
    18061 actgtgaagg ttgacgaggt gaaacaaggc gatgggccac ccctgcccaa ggacctgatg
    18121 gctcaaattg ggttttatgc gagtgatgga ccaaagcggg tttggtgccc cattgttgat
    18181 ctgcaaattc gcagtggtgc gaaggtggaa tctgctcgag aaaacgcaca accgcctggg
    18241 ggcaaccgat gacgggtgat caaacgattg tcgatcgcat cgagcaggca ttggcctcgt
    18301 tggacgccgt ctcgaacgag gagttgcacc aactggcgga ggagtacgca gaggcctgcg
    18361 agcaggtcaa tcgccgtctt caggagattc accaccttat tcgttcgggg gcacgaagcg
    18421 aggcgatccg tcgcgccgag atacctccga aactgctcga tgtggtggag attctggatt
    18481 ttcccgaccg cgacgcctgg gttgatatct gcacgctgga gcgcctgccc acgccgccgc
    18541 gcctcctcgt acagtacctc tcagaactcc atcaggcata tgaggcggaa cagggcctga
    18601 gcggattatt gcggcaacac cgtgccttgg cgatggccca ggcacctctc cacaagcggc
    18661 tggccattct tcggcaactt gcgcgggctg agccggaaaa ccccgtctgg cagaaagaca
    18721 tcaggattct ggaaaactac tggctggata aactccagcg ggaaatccag gaacgtctca
    18781 aggcagagga ccttcaaacg ctcgaggaga ttctcgagca attggaaagt aatgagtggc
    18841 tggaaaagcc ctcggtctcc ctcgtcgaac agtgccgctc tgccgtggcc tcgcttcggg
    18901 gaaagatttt tcgccaggaa atggaaaaca tcgctcgcct cgtcaatcag gccctggcca
    18961 acggcgacat ggttcgcatg gaggaatact tgcggctttg ggatcaccaa gcagcgacca
    19021 atccccaatg ggtagatatc caactgcggg aacaggtggc cgaggccatg tcccgtcgtc
    19081 accaactgga gcgcgagcga gaagaagcac gacggcgacg ggagctgcta acggagtttg
    19141 aggcccttct cggaaaaagt catgaccttc ataccctcga gcatcactac gccgagttgg
    19201 ccattcgagg tgtggccata cccgagccgt tggaggaaag gtatcgccgc gttacggaaa
    19261 cgttacggca acgggcgatg tttcgccggg tggtgaccgt cgccggggtc gcagcggtcg
    19321 tgatagccac cctcttgatt gtttggcagg tacaggcccg gatggtggaa aaccgccgac
    19381 tgagtgacgc cgtgtcggtg gtcgaggacc tcttggaaaa ggacaaactc gatgaggcgg
    19441 cgaagtatct ggcggacttc aaggcagcat acccaacact ggcccagcat cggcacgtga
    19501 tcagtctcga acaaaagctc gccgatgccc aagctgccga ggccaagcgg cgtgaaacat
    19561 tggccagcct actggaaaag gtaagggcgt cgctggctca ggttcccgat ggcgtatcgc
    19621 tacaaaaggc cagcgaactt gcccgcaccg atgcggagaa acaagaggtg atggagctgg
    19681 aatcgcaggc gaaggtcatt tgggccaacc tccgccaaca ggccgaagaa cgacttcggc
    19741 aggagatcgc caagctcagt cgcgaatatg atgcgcttcg ccaacgtggg tcggtatcca
    19801 tcgacgagga acttcaggcc atagcccgtc tcatgaatcg cctaaccgtc ctctcggacg
    19861 acaatcggcg tcaggggctt agcttggaag gggagattcg taacctgcgc gagcaacttt
    19921 caagtcgctt tctcgaagtc agtcaggcct ccgaggagga aaaactcatc cgcactatgt
    19981 gttctgctgc cgcacgaggg ccagaggcct accaggaggc gatcaagaaa ctggagcccg
    20041 ccaagtctgt gcgaattccc atcggggatt ttcaacttgt cgaacaggaa tttccgtgtt
    20101 ggaaagtgat tgccgagtgg aatcgtgtgg cgacgctgtg gaatgatccc accacctcgg
    20161 tgagcgatgc cggggcccag gagctgttca gaggagcgtt tcgactcgtc caggtggtcc
    20221 ccccagaggc ctccgcgttc gttcaagagg gacaaaaacg aattctcgca ctactcgata
    20281 cgattgcccg ccgggaattg cttgtggcgg accgcttgac ggttctcgtc aaagagtggc
    20341 gggcgcccct cgttgccgac acctggcaga ttcgtttgaa cgatggtcgc cgatactact
    20401 gcctcgaaca gccgggacga accgcgctgg ttttgcgcga tcttgggagc cttcaaagaa
    20461 atgagattac ctttccccgg gaaagccttg tttcgacagg ccgggcacca caaagtgtct
    20521 tggccaacca ggttcgcgat cacctggccg attggtctgc gagcaattgg gaaaccacgg
    20581 ccgacacggt cttggggaag atcatcggtt tttgtcacac tgtggaaaag gatactgttg
    20641 agtggccctt gatcttccat ctcctttacg aaacgtggtc ggcggccaag gagggcgacg
    20701 ttgcttgggc cgctttatgg aatggctatc aggccaagtt gcaacccctg cgggatcgtt
    20761 atccgcccca ttacgattgg atcaagacgg cccaggccgc ggagaaccag tacgaagaca
    20821 tccggagcgt tctgggatca ttgcccacgg cagaagacct gttgtcccaa gtcaagcaag
    20881 tgcgggcgac gtatcgggga aggaggtggc caaccattga tctggtcggg ctcctcttcg
    20941 aagataagac gcaggtgtgg tatgtgcgga ccgtggggca gccagccgtt cccgatgggg
    21001 ccgttctgta tgtcgtggag caaggccccg aggaaggcca agtgaccctt cgccgcatag
    21061 ggcattggag caactcccgg gccgtcgtcg aaccccagca gagtgtccgc ttttataatg
    21121 ggcggcatgt ggtgaccctc gcgggcgagg agccgggttc atgagcgtcg attcgtccag
    21181 acaacagata cacgtccgct tccgggggcg ggtcttcgga ccgtattccg ccgacgaatt
    21241 gcgacgcatg gtgcaaaaag ggaaaacggc ggccacgtgg gaaatctccc tggacggcca
    21301 aaactggcgt cccatccaag agttggaaac gttgctgcag cagagccaca gtgtgtcaac
    21361 ttggaaaggt ccctcacctt cgagccacgg tccgttgggc accatcaatg acgccgatga
    21421 cgatgtgttc gttctccgag gcacgaatgc cgaggacgcc caggacattg tgttgcccaa
    21481 tttgcaacag ccggagccgg tgacggcgga agtggtgtcg tccgaaagtc tgtggtacta
    21541 cacggtgaac gacgaacccg agggaccgat ccccgaaagc catttggccg cgatggccgc
    21601 gaccgggctc attccgcccg atacacttgt atggacggtc aatatgtcgg agtggcgtcc
    21661 gttgcgggaa acccggctgg cccgtcatct ccaatctgcc agggtggaac agccggcgac
    21721 tgtatcctca ctgccgccag ctaaagactt cgacgaagaa ccaagggaag cccnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaaacg attggatgaa ctctattcga
    21901 aggaaaaacc caagcgccgg gaatcaccct ggtcaaaccc cgtcacggag gatgagtgaa
    21961 aaggcgcgcc gccaggcaaa acttatagtt cgaggagatc aatcttattc ttctaccggc
    22021 agggccgtgc gggcacgcgg tcccaattgg gcaattgcct cttgcgtcat ggacgttcca
    22081 tccagccgca cctctttaag ctggggcatc ttgaggagga tcttgacaga ggcatccgtt
    22141 acacccgaat tgccgctgaa gtcgagccat tccaggcttt tcaaattggc caggtggacc
    22201 agaccggcat cgctcaccat ggtctgggcc acatcgagtc gccgtaggtt cggcaggcga
    22261 cttatgaccg ccagcccttt gtcggtgacg agcgtttgtc gaagggccaa ttcctcgagc
    22321 tgttgacaag ccaccagatg gtgtagcgca tcatcggaaa ccatggtctg ggtcgccgaa
    22381 agacttcgca actgcggcaa ctcgccgagt tccttgaaaa aatctccccg aatcgccatg
    22441 tcccgcaacg tcaaggaacg gagttttttc cactgcgaaa cgattcgcaa accctcgtca
    22501 ttgacagcgg tgcaacgaaa aagcgttacc gtttctagcg gtaaccgcgc caacgatgcc
    22561 aagcccgcgt ttcccgccgg agactcttct aacgtgaaac ttgtcaagtg cgaaaaaccg
    22621 cccacagcct ggaggcacac gtcgtcgatg ttgtaccccc gcagacgcaa cgccgccaag
    22681 tgctccatgg cgctgagccg tcgcaggacc tccgagttga ccgctccaca accccgcagg
    22741 tcgagcagcc ggagccgatc catttgggca attttcatca gggcctcggc ggagaactgg
    22801 ttttccaaca aataaaggtg tgtcaggcgg ggaaaaccaa cgagcgtgtc gatggcacga
    22861 tcggtaagct tgaccgatcg acgcagattc aaagaccaaa ggcggggaag tgctttcagg
    22921 tattccaggc ctgcgtcgtc gatttgggtg ttctcaagcc caagttcctc gaggttctgg
    22981 catgtcgcca actgcttgat cccttcgttg gtgacgccga agccggccac ccgaaagcgc
    23041 ttcaaatggg gaagccgcac ggccagggaa atctctgcgt cactcccact gcggcgctca
    23101 ccggccaaat cgacaccaat aagcctgccg ttggtgtcaa attccaggga ggcccgggcg
    23161 gccagcaatc cccgaataat ctcggcaaat tcagggggga ccgtaccctc ttcctcgggg
    23221 gtaccatagg ccagccgctg gacgagttcg aactcgattg gacccttctg cgcacggtat
    23281 tgcggtggat ttactggttc agcgggcgac tctcggtggg cttgttcctg acttacgaca
    23341 gactccttta ccgccggact gtcgtgatcg ggactcgctt gtcgctggca tgctgtacca
    23401 cccgtaatga tggaaaagac aaagacagcg ccgataatcc agaaggcccg aacaggctgc
    23461 agccggccga gaataatcag tttttcgcta ctagccagac gcaatgtatg attccaaaac
    23521 aacttatggc aaaggcagta acgcaaaccc ttgggcagtt tgcttatcga aatgaccatg
    23581 ctttttgttt agccattgta gccaaggtga tcatgcttgc ctattccttg gccacctagc
    23641 acagacgcat attttagctc gggcaggcac aggggcctgc ccctacgact ggcggttttt
    23701 tccggtaggg gcaaccccct gtggttgccc cgtttcgcat ggcgacacca aacgccggac
    23761 gcgggggacg ccgtctgtat ggaatcggac ggcgaggacg ccgtccctcc caatcgcgcc
    23821 ggcactgctt tgcgttggga cgggtgggcg gcggtgttgg catttgacgt tgacgcgggc
    23881 gctctgcgct gctggggcag gtacaggggc ctgcccctac acgtttcacc gggagggtcg
    23941 ccgtcctcgg cgaccaaatc actgaaggcc caattgtctg gtcacctatc cgtcttcggc
    24001 catcttgggt ccagattggc attgaacaaa catgcggaaa atggataaga tgttggcgat
    24061 tcgacataac gggttttgga caatctgctt gcccaagtga ctgaggatag tgatcccctt
    24121 tttggcatgg atgcctttat gacacgacga gtcgttatcc tgggaacgac gctgattttt
    24181 gcggccggtt gctctctttg gtttaacagc aaaaaggcct catcgctcct tgacccggtc
    24241 gagactaaga cgcccagtct ggttggtgat ttggcggtgc cgtatgggct gcatccgatc
    24301 gtggtcgagg ccattggctt ggtaacgggt cttaatggca cgggcagcga tccccttccc
    24361 tcgccggagc gacaattcct tttaacggag atgcagcgtc gtggggtaca gaatcccaat
    24421 cgggtcctgg cttccaagaa tacggccctt gttttggtgc ggggttatct tcgtcccggt
    24481 attcaaaaag gcgaaaactt cgacgtggag gtccgtgttc cgtcgcggag tgaaacgaca
    24541 agcctgcgag gggggacgct gctggaggtt cccctgcggg aaatgatggt ccttcgcggg
    24601 gccgtgcggg agggacacat cctcgccaag gccgccgggc cggtgttggt ggatccgtcg
    24661 gccgatcccg aaaaagacgc catttacgcg actcgcgggc gaatcttggg cgggggaacc
    24721 gccgatcgtt cccgaactct gggacttgtc ctcaagcccg atcatcagtc tgtgatgaac
    24781 agtgcccgta tcgaaagcgc catcaatcgt cgtttttacg tgattcgtca gggtgttcag
    24841 gagggtgtgg ccagggcaaa gactaatgag tatgtggagg tacgtctcca cccgcgttac
    24901 aagaacaaca ttgctcggta tatggccgtg gttcggtcga tcccgttgcg tgacacagag
    24961 ccggagcggc ttcagcggct tcgggacttg gaggaggagc ttctcaaccc cgcgaccagc
    25021 gagcaggcgg ccctgcaatt ggaggcgatt gggcaaccgg cggtagagac gctcaaaaag
    25081 ggattaaccc atgccgatcc ggcggtgcgg ttttactcgg cagaggcctt ggcctatctc
    25141 gactgcgcgg aggcggcagg ggttcttggg gaaatcgccc gcaacgaacc ggccttccgc
    25201 gtatatgcct tggcagccct gagcacgctg aacgatctgg agaccgtgga aattctccac
    25261 gatcttctca catcaccaag tgccgagacc cgttatgggg ccttccgtgc cctggttgaa
    25321 ctgaatccga atgattcggt cattgccggc gaaaagctca atgatcagtt ctggtaccat
    25381 gttctccgtg tgagtggccc accaatggtt cacgtcacac gtaaccgtcg ggctgaggtc
    25441 gtcctgtttg gacctgacat cacactgaaa tcaccgtttg ccctggaggc gggtcaccgc
    25501 atcatggtga catcgcagca gcctggtaag gtgaccgtgg ccaaatttgc cgtcaacgag
    25561 gcggaccaaa agcgcttcgt gagcaacaat ctggacgaga tcattcgggc agtggcggaa
    25621 ctgggcggca cctaccccga cgtggtccaa atgctccagc aggccaaggc ggcgggcgtc
    25681 cttgaagccc gcttcgaggt cgaggcaatt cccgaaactg gccgaaagta tcagggaccc
    25741 tcagaagatg aatccccctc cccagatgta gcaccgttgg tcaagaagta tgaggagctc
    25801 ccgggcgggg gcgacgtcac gacggaccac gccttgcctg gcccggagtt tgatgcctcg
    25861 gcaattgata ccgagcctac cccggcagaa gagcaaaatg tctccgagac atcgaaaaag
    25921 ggacgttctt ggtggccttt tggcgggaca aagacatctt cataagcctg acgggatgat
    25981 gagccagagc taccttgcca acttaccgac cgcaatgcca ccccaagttc cgcgattgtc
    26041 cgagcggata gtcgcggcat tttacgacca tccgtaaaaa ccggcggtgg acgaccgcat
    26101 gatgagggcg tgatgtgcgg acagggatgt ccgaactgtg tgaagtcatc aacccgcttg
    26161 aaggaaaaca ccttcggcgg gtgcggtggg gctgcgcagc gcattttggc tgtcgccgca
    26221 tcggcttccc gcgatggtct gggtaaaacg tcaggaaaac gcacgccgtc ggctgctggt
    26281 catagtgtcg tcggctcatt agatggaaag gcacgctttc cgaacatcgt cgcggagttt
    26341 acccgctggc cgccgttggg gaaccgcctt tggacctact gcgtcgtggc cgtggggatg
    26401 atcctcagcc tgtggttctc ggtcgctgct tgcggggagg agaatgggct gcggatttcc
    26461 tggggcgggg gctcggccag gcgctgggcc ggtcggatca gtgtttccca aggggaaatc
    26521 ctccgctggc aacccatcgg gtcagaggcc gacgaactgg cctccctggt gtttcgcgat
    26581 aaggggatcg acattgtccc caccactccg cgggtctttc agggggtgga cctcgcactt
    26641 gatcggcagg gccaaacgct gttggagatc gagctggcgg ccgagggtac gccatcgcaa
    26701 cagtggtctg tgcccattca agagatcgaa cgccaaccgc gccagatggc actgtcgggt
    26761 ggcggacagc ttttcatcga gcggcttccg ggagacgagt tggccgtgtc cacggatcgt
    26821 ccccacctcg ttttttcgcc cggggaaaca tggaaattct acgtcacgcc tcgtggcttg
    26881 cccttctcgc cgggaacaca tgtcgatatt cgcatccata tcgtccgcag ccggataacc
    26941 gatcgagagc cgcgtcggcc cttgctcgac tccttggcag acctacggcg aacgggcaac
    27001 acagatgcgc cggtggtccg ttacgagatc accgtcggta aggaatcgcc catccctgtg
    27061 gaaattacgc tccccgagga agaaggctgc tatgacgtgg tgatcacggc cacgccgatt
    27121 cccagttttc gtttggccga gtcggtcacg cgtccttttg gcgggaatgc ctcgaacctg
    27181 gaacggcatg tgcaagtggt tgtgctcaac acaacgcggc ggacggggga ggccttttcc
    27241 ggagagttga ccctcttggg ggaggccgtc gatgccagca atcagaattt ctgggagcgt
    27301 ctatcccggg tggcgcctta tttgcctcgg ctggggttcc tcggtcgtgg ttggcggcac
    27361 attgggggag aggggcaaga ggcggtctgc gagcttgccg cggccagccc gggagaagat
    27421 cccgcctggg ccctgtactc gcttcccgtt caggaacccg gccgacctca cctcttggaa
    27481 atcgaatggc cggctgacca gcggcagacg atgctctttt ccattgtgga gccgaacgcc
    27541 gtgggcgcca ttgctccgcc catggtcggc tatggcgtct attccaccgg ggaattccgc
    27601 cggtgggaac aaccgctcgg ctggcaggtt catcgcgtcg tcttttggcc acgaacacgt
    27661 cagccacttc ttttggtggc caatccgagt ggcgaaccgg ctttgtacaa aaagattcgc
    27721 gtttatgcag gatggaaccg cttgccggaa atctttcccg cctctgcctt ccccagcgaa
    27781 cggctggtgg cggcctattt ccatcgcccg attcttagca cgctctttgg tgccaccgat
    27841 gtccccggtc ccttccagca gcagggcttg gacgattggg tcacctttta ccaagcggcg
    27901 acccgattag ctgaatacgt gacgtttgcc ggatacaacg cggttgtctt gtcggtggct
    27961 gccgatggct caacgcttta tcccagtgag cgttggaaac cgacgccgcg ctatgatagc
    28021 ggttcgtttt ctctcaccgg ggccgatcct atccgcaagg acgtcctgga gttgatcctt
    28081 cgcatcgccg accgcgaggg attttcggtc atccccgcca tcgatttttc cgtccccttg
    28141 ccaaatcttg aggagacgat tcgaaaacag ggcgagcggg ggcgaaacct ccgctggatc
    28201 ggtcccaacg gccaaacgct cacggagaca caggcgcctt atcgagggat ggcgccctac
    28261 tacaacatcc tccaccgcga ggtccaggaa gagcttctgg ccgtcgttcg tgaacttgtt
    28321 gaacgttatg ggcgtcatgc ctcattttat ggattgggga ttcagatctc ggcctacggc
    28381 tacgcacaac ttcccggacc agaatgggga ctggatgacg agaccatcgc ccgattttct
    28441 gaagagacag gcatccaact tcaggtgccc gatgataacc gttatcaaga acgggcccgc
    28501 ttgcttttgg gaccttattc ccgcgaatgg ctgaggtggc gctcgctgcg acttcaaacc
    28561 ttttattctc gcgtgacaca agaggtggca gccgttcggc ctgatgcaaa ggtcctcctt
    28621 ttggctccaa ccatgttcgt gggcgaacgc tgggaacagc gtctccgtcc aacgttgacg
    28681 gaacgtcgtg acccgctggc cctcctgcta gaaacgggat tgacgcccga gctggccaca
    28741 agcgacttgg ccatgggcct cctccgcgtc aaacggatgg ttgagcatgc ggatttcggc
    28801 gttcgcacca cggagtttga gattgaccaa atctaccggt cgatccccat gaacgcccaa
    28861 ggacacatgc ccggcgtgtt gttctatcat ccgccgcagg aaattcgttt gcctagcttc
    28921 gatgcccgat cacccattca gccggccttc acggccatca ttcatcagcc ggtggcctgg
    28981 gggctagaaa accggcgacg gtacgcccaa gccctggccg atggagaccc cacattgatc
    29041 tgtgacggcg gctgggtgac cccttggggt ggcgaggata tgctgcggcc gtggcttgcc
    29101 gccttccgcc gtttgccggg gggaccattc caagatgtcc gtttccccga tcaagggacc
    29161 tcactccaac cgctcgtggt ccgcagtttg acacgccagc aggagaccta cctctacttc
    29221 gccaatcagg tcggcttccc cgtgattctg gagatgacgc tccaggcccc gcggggctgc
    29281 cgcatgatcg aactgagtgg cttccgctca ttaccgcctc tggaaagcga ctcccaggga
    29341 cagtgccgct ggaagatgac ctttgagccg tacgatctcg tggccgtcaa actgacgact
    29401 gccgacatca cgtttacatc ggccaaggtg agttggtcac ctaaggtcga acaacgactg
    29461 cgggaacaga tcgccgattt gagtgaacgt gctgccacgc tcagcgtccc tcccctttat
    29521 gccgtccttg aaaatcccga ttttgaagag ccggccggtg acgcgcaacc ggtgccgttg
    29581 tggacggccc tcgcacccgc ggggacaatg gtccgagtcg atgcggcgga aagacacggt
    29641 gggcagcaat cggtgtatct catgagccaa ggccccacgg tttccctggt cagtcaggcg
    29701 ttcccaccac ctcccacggg acggctgacg gtcgcggtgt ggcttaagac gtcccatcca
    29761 cagagacagc caccgttgcg gtgcgttgtg acgggagttc atcgtgggca accgctgagt
    29821 cgcatcgccg aaatcggtct ggcgtcgccc ggacggactg cgccggtcat ctcctccgac
    29881 tggacgccga ttctcgtgga ggtgacagac ctccccatga gcggcctttc gccgttacag
    29941 ctccgctttg acctggccgg gcagggtgag gtctggatcg atgacattca actgtgccat
    30001 cttgccttta gtaaggcaga gcgggtggag cttttcaaac tcattgcccc ggctcaggct
    30061 aaattggaaa atcgggatat cgccgattgc ctgcgattac tggaaggttt ttggccagag
    30121 tacttggccc ggcacgtccc acgcagcgag ttcagtgtga cggccagcga ccaaccccgc
    30181 gagccaccgg agagtaaacc gtcagggaag cccgctgaga aaccacccga ccgctcggcc
    30241 ggtctcctcg accgagtgcg acacatcctt ccccaagcgc tgcgattctg aagcggtgac
    30301 caggaaagga agtgacggtc acttcggctc ttcgatgctt atggtcagga cgtttcttct
    30361 ctggtagccg ctgacctctt catgagataa cgactcttcg ggaaagtcca cttcacgcgt
    30421 tggcattctc ccagctttta ctcgaagatt ctacccacct gggaagcgaa caggctcagg
    30481 ttgcctagag ggtcgagctc gcggaagccg ccatcaattg tttctccttc aatccgcgcc
    30541 tcgtgaaact cgatctacgc acgccaagag aatggccttg accacagggc gatggctctc
    30601 ttatgatccg cctcggtatt cgcggtgaag acggcaaaag agtgattgga acctgaggaa
    30661 gatacgcgga cgagaaaagg tgaggatgcc aaagggcgac gtgctccacg agaggacaag
    30721 gaggatgacg caggacgaac aattcccgcc ggggtttgtg gacattcatt gtcacctttt
    30781 gccgggcctt gacgatggac ccgccaactg ggatcaaacg ctgaccatgg cccgggcggc
    30841 ggtggaacac ggactggtgt gggtggtggc cacgccgcat caaggagggc gatataccga
    30901 gaacacgcct gcacgcattc aaagcgttgt ccaggaggcc aaacgcaagt tggaggagca
    30961 ccaaattcct cttcagcttt ttgctggggc cgatgtccgc atcgacgaga ccctggtcga
    31021 acgggtccaa aacaaagagg tgcaaccgat tgccgatggc cggtatctcc tgctggaact
    31081 accccacgat gtgtattttc ctttgcaggg gcttctcaac gatttgcgac ggctggggat
    31141 aacgggcatc ttgacccatc ctgaacgcaa tcacgcgatc attcgccgcc cggagcttct
    31201 taacgaaatc ctccaggccg gggccctcgt tcagatcacg gcggggagta tcacgggagc
    31261 ctttggggcc gtcgtgcgac gattcagcga agagttactc ctttcgggaa aggtccacgt
    31321 cgtggccacg gatgcccatg ggcgtcacgg ccgtccaccg cacttggggg acgcccatcg
    31381 ccagattgcc cggctgcttg gtcgagatac ggctgacatt ctatgccgcc gcaatccggc
    31441 tcgaattgtc ctcgggctgg caccccaaaa gctggaaccc gtgcgtccca tccgttctct
    31501 gtggagccgg gcgtggcgac gccttgccgg atgataaggg ccgggagcca ctgccgtgta
    31561 tccgcccatc cttcttgccg tgatgtgttc ctcggcgcta cttggccagg tggcgtactc
    31621 gccctgggtg gaaacacgct cggtgcgggg gctgacgatc tactctcagt tcccgctcca
    31681 ggacgttgcc gacgtccaaa ccgatttgga atacctggcc gaggatatcg agaccgccct
    31741 gggcctccgt ctggccgaca ggccggtctg cgtgcttctc tttgccggac gccgcgagta
    31801 cgaccgcttc ctcaaagagc ggtttcccga cgtgccctat cggcgggcgc tttatatcca
    31861 acggtcgggt caggcgttcg tcctggccta tcgcagcagt gaacttgccg ttgatctacg
    31921 tcatgagtgc acgcacgccc tgcttcactc agccttgcca gtggtgcccc tgtggttgga
    31981 tgaaggattg gccgagtact tcgaggtccc ccgtgctcaa cgggccttcg ggcatcccca
    32041 tttgaagact atacggctgt gggccaatct cggttatgtc ccgtcgcttt ccacactaga
    32101 ggccatcccc cacatggacg ccatggacgc gaacaaatac cgatggtcct ttgcgtgggt
    32161 ccatttcatg ctccatgggc cagctgtcgc tcggctggaa ctggtggatt acttacagac
    32221 cctcgcccaa tacggtgttc caaggccctt aagcgagcgg ctcaccgccc gtttgggtga
    32281 cccccgccgg gcatatctgg atcacttcaa acactggacc cgctaggccg cttgacttcg
    32341 gctgctctgt aagcatcgga ttgaccgccc ctttgctcca atacgcctta ccgcagggtg
    32401 tcatccggta taggatgaca taatgtacga gtttggtccg acttgccggt gaggcaactt
    32461 cacccgcgtc tggccctccc gaagcccctt cgttgatcgc ggtgtgacgc cggcggcaga
    32521 ggacccggac atttccccat agcaaaaccg gattcactga atggacgatc aatccaacgc
    32581 tttacccatt gtttcgaccg atcccgaagt gcaggcctcc cctgcgagaa gctcggcaaa
    32641 acccctgggg acatgcagcc tcattgccct cggctgtccc aagaaccttg tcgatggcga
    32701 gcggatgttg ggccttttgc gcgacgccgg ttggcagctg accacagacc cccaagaggc
    32761 cgacgtggtc gttgttaaca cctgcggttt cctccgctcg gcccgtgagg aatcgttcgc
    32821 cgtcattgag gagatgctca agctgaagcg gaagggcaag attcgtggcg tcattgtcac
    32881 gggctgtttg gtggagaaag accgggaggc ccttcttgat cgcttcccgg aggtggatca
    32941 actggtcaat ctctttgccc gcgatgaaat cacgcaggcc gcagcgcgga ttcaagcagg
    33001 tctggtcgaa caacgaacgg tcttccaacc ggcttccgtc gttcccctgg atgatcgtcg
    33061 tcgcttgcgg gtaacacccc ggcatttggc ctacctgaag atttccgaag ggtgtgaccg
    33121 cctttgcaca ttctgcctga ttccccacat ccggggacgc catgtgagta agccgctcga
    33181 gagtattgtg gaggaggccc gggaattggt tgccgagggc gtgcgggaat tgatcctggt
    33241 agcgcaggac ttgacatact acggcctcga cttggcgggt cgtcccttgc tggccgagct
    33301 tttgcggcgt ttgcgggaat tgccgggcct gcggtggctt cgccttatgt atctgtatcc
    33361 catgcacatc accgacgaac ttgtggaagt cctccgcgag ggtgagccgg ttctcccgta
    33421 tctcgatctg cccctgcaac acatcagcga ccgcatcttg aagcggatga agcggcgggt
    33481 caatcgccgg gagaccgagg ccctgttagc gaaactgcgg gagtcgatcc cgaacctggt
    33541 cctacggacg acgatgattg tgggctttcc cggcgaaacg gaggcagaat ttcaagagtt
    33601 agtggagttc gtcaagaccc agcggtttga aagactgggg gtttttgagt attctcgtga
    33661 accggggaca ccagcggcgc ggcttcagga tcatctgccg cgtcgggtca tccgggcacg
    33721 gcgggagcac ctgatggccg tccagcagga gatcgccttt gagtatgccc agggccaggt
    33781 cggacgtcag atgaccatcc tcctggacca gccgtatcca gagcgaaggc atgtcttcat
    33841 cggtaggaca tacgccgatg ccccagagat cgacagcgtg gtgtatgtga cgggaaaaca
    33901 ccttcagccg ggacagtttg tcacgtgcga aatcgtggca gcccagggct acgacctggt
    33961 ggcggcacct cttgatgaac aacctgcggg atgatgtctt cgatggcttt agattcttat
    34021 gacctggtgg gaggtttgcg atgagtcgca tgacacggcg tgactggtta aacacgacgg
    34081 ccctcggtgt cggactgtgg tcgatgggtg ctctggccgg acgaaagccg gtaagtgggg
    34141 aggaagcggc gaggcccgcg cgacggtttc aggcggcgct tgtgtgttcg gccattggtg
    34201 tgcgggcgga tcagcgtcag gcgattgaat atgcagcccg cttcggcttt gaagccgtcg
    34261 aaccgcagcc tgatttccta agccgacttt cccaggagga gctggccgac ctccgccagg
    34321 aaatgcaaga aaaagggctg acctggtcgg cggccgggct tcctgtcgat tttcgcggtg
    34381 atattgacgc gttcacccgc tccttgcgac gtttccccca attctgcgaa accctgcaac
    34441 gcgccggcgt ggatcgggtg gggacgtgga tttcgcccag ccacaacgaa cggacgtatc
    34501 tggaaaactt ccgcttgcac gccaaacgac ttcgcgcggt ggctaatatt gccgggggtt
    34561 acgggctgcg gcttggcctg gagtacgttg gcccgaagaa atcgtggtcg gccggcaggt
    34621 acccgttcat tcacaccatg cgagaaatgc gggaattgat tgccgaaatt gactatccct
    34681 gcgttggcct gacgctcgat tcctggcatt ggtacacagc aggggaaacg gtggaggacc
    34741 ttcgcaggct ccctggccat cgtgtcgtca tcgttcatat caatgatgcc ccgagtggaa
    34801 ttccggtgga ggaacagatc gactcggtcc gcgagctccc ctgtgccacg ggcgtcattg
    34861 acctgaaagg gttcttgggg gcactggtgg aaatcggttt cgacggcccg gtttacattg
    34921 agccgttcaa aaaagaacta cgacaattgc ccccagagga ggccttgcaa cttacggccg
    34981 aggctatgca gaaagcgatt gccttagtcg gaaactgaca tttcaaaggt cttgcgaacc
    35041 gaccgtgcgg ccaagaaaag acgatggccc tgtggggcaa ccaacagggc catcatctgc
    35101 aaggatcggg aggtgtcggc aagggaatac ggaggttact gcgtggtact aggcacttcc
    35161 tgcttcccat tatttgaagg cgtgcctgac ccccgctcgg cgattctccg gtcacgggct
    35221 agaaggttct tcgtcgtgct cggcaacggc aaccgggacg gcaatggccg ttgcgatggc
    35281 cccacccaca atccagggat tccgcatcca tgtccggaca cgcggcagga cctgaccgcg
    35341 ggcaaggtct tgacccgaga cgatcaacgt ggaatcctgt gcgcctggag gagctgtccg
    35401 tgccgcccat aggcgataca tccctgtccc gttggccgca ttcagacgat aaacaccgcc
    35461 cttcaggccg cggaaggcaa agcgtccctg ggcatctgtc gtggccgtgc caaggacctg
    35521 atcaccactg accacggtca cggcggcatt cgggaccggc agaccctcgg gcgtcaccac
    35581 ttgtcctaaa agtaacccac catcatggag ggctacgtca atgacccgcg gggccgggga
    35641 ttgtttgctc tccactgccc aaagctggga agagcaggcc accattgcca cacacgcaac
    35701 aaaaactgaa gttctcatct ttcttactcc gcctccgtac gagatgaagc gaccttgccc
    35761 gggggtacgg gaaacgctgc aaaatcgaca acgtccttgc agcacaatta agcctcacgt
    35821 atggtatcgg caggcaatgc ccgatccttc agccaaatcc ttcaaaacgg tccctaatcg
    35881 cccgcaaggt cgccccgccg catgatgcca caccggttcc cgggactccc cgacatggcc
    35941 cgggttacca tgttttcctg ctctcgggcc taattttgaa acttgcgaga ggggaggaac
    36001 tcgggtatct ttgttgagga gctgaaggcg gctcaagcgt cgaaattaac catgggagtc
    36061 tgggaggcag gagtggccaa gcgggataac cattacgagg cggcctttga gtcttatttg
    36121 cgcactgccg gcatccccta tgtggcggtg gatgaggccc ggcgggcggt catggccgat
    36181 ggccgatccc tcaaaagcct cgatttcctc gtggcaccac ccggtccggt cacctggttg
    36241 gtggatgtca aggggcgacg cttcccctcg ggcctggagc atcatcagta ttggaaaaat
    36301 tggtccaccc aggacgattt ggaaagcctg atggaatggg aagtcctttt cggccaaggc
    36361 ttccgcggat tgtttgtgtt tgccttccac gtcgtgggaa atcgtctccc cgtcagcccg
    36421 gagtggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcc ccgttagccg
    36541 agcaaccttt ggagatgctc gatgagggcg gagcactgcg gatccagagg cacctcgctt
    36601 tgtgtcccgg cctttaaatc cttcacttgg cacacgcctt ttcggaattc gtcctcgccg
    36661 atgaggatag ccacacggtg gccgcgtcga tcggcgtact taagttgctg ggccaacttt
    36721 ttcggctcgg ggtaaagctc gacggcaagt cccgcggtgc ggagttgggc cgccagtcgc
    36781 agatagtcat ctcggtgctg gtgatcgaaa aagacgatga agaccggcgc ggtcgttgac
    36841 accgcttcca gcatccccag ctcttccatg gcggcgagga ggcgatctag acccagcgag
    36901 gccccaacac ccggtaaggg ctgcgtcgtg aacaaggccg cgaggttgtc gtagcgtcct
    36961 ccggagcaga cgctcccaat gccgggcaag gccgccagga aggtctcata gatggtcccc
    37021 gtgtaataat cgagtccccg cgcaatggcc agatcgacga ctaggcattc ccgggccacg
    37081 ccaccggcca ccgcgccgcg gacgacctca cgcaattcag cgacgccctc ttcgcccacg
    37141 gcgctcccct ggacaaggcg ttccgcccgg ctgaggacct catccatgtc accgtgaatc
    37201 tcggttaagc gcaggacctc ggcggcttgt tcggcagtca gtccggccac ctcctccagt
    37261 tcccgcttga cgccctcgga accgattttc ccccatttgt ccaaagcgcg aaggatgagc
    37321 accgcccggt cacttagccc gaggcgttcc aaaaggccgt tcaacacccg gcggttattg
    37381 atgcgaatcg taaattcccg aaagccgatc gcccgcaaca ggtcgtgaat gacaagggtc
    37441 gtctcgatgt ccgaggtcaa cgagtgggtt ccgatcgtgt cgaagtcgca ctggacgaat
    37501 tcacggtaac gtcctcgttg ggtgttttca cctcgccaaa ccggggcgat gtgataccgc
    37561 ttgaagggcg ttcccaactc attgaggtgt tgcgccgtga agcgtgccgc cgggaccgtc
    37621 agatcaaacc gcagagccac ccaacggccg ccgtggtcct ggaagcggta aagctgcttg
    37681 tcggtttctt cgctcccctt gccggcgagg atttccagat actcgagggc gggcgtgtcg
    37741 atcggcacaa atccgtacga ccgatagacc cgtcgggcaa tttccatgat ccgctcccgc
    37801 gggatcatca tttgcggaag ataatcacga aaacccttca gtgttcgtgc tggaatgagt
    37861 gttcccatgg tcgtttttgc aagacttctt cacttttgag ggtcagatat gtaacgcctc
    37921 agcaggtctt ccggccagtc atttcgcccg gccatccgga caggtgacat gttccgcgac
    37981 ggctttttcc gccatctcta caatcttcaa gggattgggg cgaggcgaac ggatcgaacc
    38041 accggatcgt gggccgagaa ttgcctcggc atattcctcc agttgctcgg ccgtctcaaa
    38101 aaactggtca tagacggcgt tttcttcaaa ttcgcagatg tccattccgt agtcacggca
    38161 caccttgggg cgatgggcat agatcgcaca ccggccatcg gaccgcagcg ccttgcacgt
    38221 cagataaacg agcaaatacc actgaccgtc atcgacgaac acggccgtcc gctcatggag
    38281 caagaaccac cggacggtgt cgaacccctc ccaggtccgc ggtcgatcga tcggcagggc
    38341 gatgtactgg cagcatttag cggggcagtg aaagcacagc acctcgcctg gggggacgtc
    38401 ctgccgccgc aagcggccgt tccgcacggc tgccgacgtt cgcgtcaacc agtaattctt
    38461 gatcggcttc ccgttggtcg ccatatcgtc ccgcagtcgc tcccatccat tcttctcagg
    38521 acatggcatg aaatcatcct ggaccaaaat attaggccca ggtatcccct tgaattgact
    38581 ttaccgcaat taccggacgg cgataagggg ttgccgacag acgactcggc cttagactgg
    38641 cacgcatttg atcgaaggac ccatccgcgt tacttctcgt ctggggcctg atctggccgt
    38701 tgagcctgct cccgtgaagg cggtggcatg ggcgattcgt aaaacaggtg aacatcccgc
    38761 tgcggaaatg ggatggtgat gccttcttcg ctcagccgcc gatacacatc cgcgtggagg
    38821 ctgttgatcg cggttaggcg ttgatcgtag ttgctgacgt atgcaaacag ccggaaattg
    38881 accgagctat cgccaaaacc ctcgaagacg actgcgggcg caggatcggt catgacgtgg
    38941 gggttccttt tggcggtttc tagcaagatg tccatcaccc gctggggctc ggtcccgtag
    39001 gccacacgca ccgcgacctc aacgcgattg acggggtccg tcaaggtcca gttgatcacc
    39061 cgtcccgtga tgaattcctt attggggata atcaattccc tccgctcgaa attgaggatt
    39121 gtggtggcac gcatgcggat acgtgtcacg cggccggaaa actctccaac cgtcactagg
    39181 tcacctaccc gaatgggccg ttcaaagagg ataatcaagc cggaaacgaa gttggcgaag
    39241 atctcttgga ggccaaagcc caaccccacg cccagggcgg ccacaagcca ccgggcctga
    39301 gaccatcgaa gacccaagta cccagccgcc atgatgaggg cgaccccggt cacgcaatac
    39361 gatgtgattg tctggagggc gtatctcagc cctggctcca ttggcaaccg atgggaaaag
    39421 accatctcca tcagtccagg gatgttcttg gcaataagca atccggccac cgatgttatg
    39481 acggccagaa gcagatccgc caaggtgata gccggtgtgg cgagggcgat cgggcccgcc
    39541 tgggagggct ccgcggagct ggtcgaaggt gcagtggtca cggcagtcat tgggccaccg
    39601 cgccaaagac ggacctgcct gaggaagccc agcgcgggaa ggatgtcggc ccaaatccag
    39661 ccgagggcca ccaacaccac caagacctcg atcgtattga agagccgccg tgtctgctga
    39721 ccgacgacgg ccaaatcggg ctcggactcg gcctgcgaag gagagacccc gcctacttcc
    39781 gttttggcct caccgacgat ttggggctca ctggccatgc gctgccggaa ccgttcccag
    39841 ccgatccgtt gcttgcggac gatcaaccag cggttgaaca gctcttcgcc cactcctacc
    39901 aagaggaggg ccatcaaact gccgcgaaaa cgctgcatga gttgcaccgc cgtatagctg
    39961 taaccggcaa gtgtgagggc catcaggcca acgggcacgc ccaccgcgag caaccgccaa
    40021 agtgttcgcc cccgataaag ccagctctgg ggatgatggg cacgcacggc ctgaaggagc
    40081 gatcctttgg gccgaagcat tatgtggccc agaaccgcgt aaaggaggag catcagcaag
    40141 aacacaatcc gccccggacc cgcctcccac cgctggtcac ccatggcgtg gatcaccgtg
    40201 caaaaataga ccaacaccat gcccgccggt atgaaccacc gcaattgacg gcgaacaaac
    40261 agggtgagct cgctcggcca ccgaaagtgg gcctcgccca atcccaaggg aagacataac
    40321 cgccgaagcg ttccaaagac tatcaagaat tcggccgtgc tccaaaaact gtgtgacatg
    40381 gccaccgtca tcgtggtggc gttgataggc gagcccaatc gccaggccaa gaaggccaaa
    40441 acggcgggcc accatccggt ccccaagact gttagggcaa ggacctggag tgttggccaa
    40501 aaaccgtgat aggccccctg agagaccttc ttggcgagcc tttccagatg acgcctttct
    40561 tgaaagagtg ccaaccgcca tacggccaag accaaccctg ccaaaaccca caaggccggc
    40621 catgtcccta agtcgatttg cagggcatgc cccgcatccc aaagcgttgt gggcgagacc
    40681 aaacgacgaa ggacccccaa tgtcagacgc agatcgtctg aaagctgaga gagacgcaca
    40741 ggccgcgtgt tgggaatcca gaggatgtgc tcatcaatat agcgtcgata ctcctcgacg
    40801 gtcctgacaa tcgcgcgctc cgtgttatcc aaatccacca gatcgttgag ccaccggctg
    40861 tagagttgaa cttcttcatc cagcaatgtg tatttctcgg cgagaacctc tcgcaagttt
    40921 gtttcccact cgggatggtc ctccgaaatg agcgggagaa gccgattccg ccaccgagtt
    40981 aattcctcct ccaaaggagg catttggctg tgagcgtcac tcaattccaa gagcgtccac
    41041 tgtgtcgccc gaatgtcatc ctgtcgtttg cggattcgtt gccagagggt atgggtttcc
    41101 ggaagtttgt tcctctgtgc cctcagccac agccccaagg cccccgtcat gccgaccatc
    41161 tccatacgct ctttggtggc cttgaactgt tgctgaagct cttccaactg ttgtttgacc
    41221 gtggtgagct gaacgtgcgt ttgattgacc cgcgttgtca ggtcggcaac ttctccggcc
    41281 tttttctcca cctcctcggc cagtgcccgc agcggttccg gaagttgggc aatccgtgcc
    41341 cgggcctgct ccacccggga gaccgccgtg gcctgtcgcc gttgggcaag gatttgttgc
    41401 cactgattcc aaaccgtttc caccgtacgg aagcgctggg ccgccacccg tctccgcagc
    41461 ggcaagaggg tttcgtcggc tcgatcataa taggccagtt cctccttaat ggcctccagc
    41521 tcccaccggg ccgcttcgag ttcagtttga cgcagaatcc gttgggcctc cgtcaaagcc
    41581 ggtggctggt tcgggtcggg agaggcagcc aaggcctcgc gggctgcttc taaccgtttt
    41641 tgggcggcct ccgcacgctg agtcgccgtt tgccgggcgg catttctttt ggtcacttcc
    41701 tcatctatgg cgtcgcgttg cagccgggcc gcattaagcc gcttttccgc ctccgccact
    41761 gccgcctgaa tttcctctaa cgtcatcccc tcggatggtg ggaggaacgg ggtggcactc
    41821 acctcggcca gctctgcctc gagtcttttg acctcctctt ggatggcctc ccgctgctgg
    41881 cggaactgct cggcctgatt tcgaaacttc tcggctgccg ccaattcttg gatggcccgc
    41941 tcgtaggcgg ccaccacctg tgacttaacc gcgtcgggca actcaccgtc tgcctggacc
    42001 cgctggatgc gcagcgtgat ctcctcgctc gtgatgcggg ggatactttc cgccacggtt
    42061 tccgacgtcg gcgcctccgt ggtgggcttg gccagtggcc caggagtttc cgatgatgcc
    42121 ccgggagacg atgtctgccc accttcgtcg tctggaggag gcagccgcag gagattcctc
    42181 aagggtggtt cctgagcggg acttctcgtc acacccccca cggaatgcca tacggccagc
    42241 gccacgacaa tactcaccac ccacagtggg cagctcgttc ttaggacgct ccaaatggtt
    42301 cctagcgcac ggttcattgg ttatggctgc ggatgatcgg ccagcttgct ggacgtgtct
    42361 ggttcgttgc caagccagca cgccaccaaa gttcttcctt ttcacgtggt aaaaaacgga
    42421 ttcctgttat agactttccc gttggaaaca ggggaaacac caggcccaaa tggcattgtg
    42481 gctgtctcgg gccgaggatg caatgtcaat ttaggaatcc ccgaggccta cctgtggaaa
    42541 gagtaactga catccgcgcc tgatggtctg tcgatgatcg tcctaactcg ccgaaggttc
    42601 aaggctacct gcacccgtct gtggcaggag aagatgctaa tgtgactgtg ctcacggatg
    42661 aaccagcttg aaggtatagc tggcgagtca gtccaagaaa cgaaaagcgg ggcgtccttg
    42721 ccccggacca ttgcaacgtg gaggcgatca cttcaagctc acgcggacga cttacagtct
    42781 atcccaaaaa gtccccctct ccctccggga gagggttggg gtgagggcaa gcgatcgtcg
    42841 gagtcgattg agaaaaccgg cagcgatttg agccgccctc accccccgcc cctctcccgc
    42901 cgagcgggcg aggggaggcg gtgggacagg ctctaaaatg ggccaccaca cctcggcccg
    42961 aaccactttt tgcaaaacga aaaaccacgg gcacggtggg gcgcgtaccc gtggtcttgc
    43021 gggaaaaggt gacgatgaga gactccgtta cttcacatcc gttgccctct ttcaaccgac
//
LOCUS       DAOSUT010000007        42824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_621_length_42824_cov_9.867310, whole genome shotgun sequence.
ACCESSION   DAOSUT010000007 DAOSUT010000000
VERSION     DAOSUT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 42824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42824
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_42824_cov_9.867310"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2258
                     /locus_tag="PLD05_01270"
     CDS             162..2258
                     /locus_tag="PLD05_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOP76084.1"
                     /translation="MGYPQFDRSRLILRPLAERTHDIDRSILLDPTTVQSSFHHPAIP
                     TLAVRILEARKRGAAVIAFCGAHVLRTGNGPLLIDLMEKGLLTHLALNGAGAIHDFEL
                     ALIGATCESVARYIRTGEFGLWQETGRINDAAIIAAREQIGFGEALGRMIETERFPYR
                     ETSVLAAGYRLRVPVTVHVAIGQDIVHEHPNFDPVAIGQATYTDFLIFTHSVANLEGG
                     VFLNIGSAVAGPEVYLKALSMARNVARQEGREIRDLTTAVFDLVAIGSDWRTEAPKTD
                     PRYYYRPFKTVLVRTVADGGTSFYVQGDHRETVPALYRTLTTFQKELDTLDTTVEWRS
                     QFERRGASRMKQVERFAGFRKDRLEEILSRFKNLRVAVVGDFFLDKYLDVDPRLAETS
                     LETGKLAHQVVRVRHSPGAAGTIVCNLAALGIGTIYCVGLIGDDGEGYELRRDLVNLG
                     CHIEHLHVCPDAMTPTYLKPRDITDPSLAGEHSRYDFKNRSQTPPELRQKIANSLSTL
                     LPQVDAVIVLDQVEEADCGVVTKDLRELIAELAPCHPGVIFWADSRQSIREFRHIHLK
                     CNQFELLGNALDPWRPVPDWDILIPAMEEFRKVNNAPIVVTLGKDGMLVSDPAPTWIP
                     GVRVSEPTDPTGAGDSATAGAVASLAAGATLPEAALVGNLVASITIQQLATTGTARPE
                     ELPPRLEMWLEQQAQT"
     gene            2356..4890
                     /locus_tag="PLD05_01275"
     CDS             2356..4890
                     /locus_tag="PLD05_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOP76085.1"
                     /translation="MSDWKPTITHHLTKWLRRNKPAIRALLGDFLQAIEIEQFLALGT
                     SQNTFYDSTKEDDLTQSWAAEWGQADHIPLAAFPVIVAQFSSWLITAVAVHGLSHWNV
                     PDLPKSPDLFANEVMRWLDEDCARRAGSSGLWHLLDWLGIRLPCIANQGDAHRFFKHL
                     AEWAGQFFEQWHQQTDTWCDLFQPLHTVLVPKKIRLRMGEFYTPDWLAEHILAELDYP
                     GSDASRLFDPTCGSGVFLTAAVRRLIPHLLAPHNGWKTGGRSTGSQSPQTRACPESLA
                     LEVVRGVLKDQIVGMDTNPLAVVAAQANLLLATIHTLWRICPAMRLPLDAVLDHPSPI
                     RCGDLLDSSWETPTANARPMGPGAERQTKPQIQPQFHFLVGNPPWLLWDNLTASMRER
                     LAPLCQELGLFDLSGREARHGGAKRDVGVALVWMATQYLLPGGKMGVVLPATLFRGGN
                     AGRSFRRFTVGPETPLRVLQVEDLSGLPVFPGTIRRTVLFFAEKGFPTRYPVPYYVWH
                     KEAGQREMDNGRLHASRTKLVAVPSCPDDTAAPWSITTESARNIWQKVAGPSEYSAYL
                     GANTAGANGVFWLEIIGHECGLAVVRNCAESGRRATPHFQGPLEPDLLYPLLRWKDVI
                     PAGVASTVILLVQDPLTRKAIPEELLKARYPLTYAYLSQFRDILEARAARRKLHRKEP
                     WYSQYNVGHYTMAPHKVVWRRMDWKFRAVLVGPQPLGELGVRPVVPQETCCFIPVDSV
                     EEGAYLLAILNSRLVREIVQASALPGSRGFASPRILRTLAIPRFDPRVDLHRRLAEIG
                     QKASSTGQVSCDKWLEANNLTDWDRCLAELYGLSPSDYVALCEKSV"
     gene            complement(4918..6174)
                     /locus_tag="PLD05_01280"
     CDS             complement(4918..6174)
                     /locus_tag="PLD05_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76086.1"
                     /translation="MITSESSSEALAAGEQVVRLLHEHRARCREVLSRQQERFRLAEQ
                     ELGDYLGGLAKTVEALRAELSQRESKSSEKILPLLSALDSAVRSIQERGESLRTENEN
                     RSQLYAQLTAELAAMRREMAELRSIKAHLEQQLAISQSEKQTDAEQWQRREAQLRAEF
                     ESEKDRWRAQWNDRESAFNALVIRCSELEEELEKLRRQSDPQASSDLQQRYEMAIVEI
                     RELRQKNAELEKKLAQIEQSGTKPVVTSSDGRILDWEAEKQRILAALEAEAQEDSNPE
                     RAAERIRIQDVIKKTEAALAEKDREIAELRRLLEDQSTSIGSVAVGAAAIERLFDQDE
                     VLREQREHLRQLEEEWKEKLRKAELEISLERAKIARERAVLEEKQRELEEKLAALGPS
                     KSPEEEKNKPSRGRWLSRLGINGSSP"
     gene            complement(6703..8268)
                     /locus_tag="PLD05_01285"
     CDS             complement(6703..8268)
                     /locus_tag="PLD05_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOP76087.1"
                     /translation="MRISSNRWRLMWGLSATWLVLIVAGSLLADVKPVALFCDHAVLQ
                     QQMTIPVWGTADPGEEVTVRLGDQKVTTKADNNGDWMVKLQPLPAGGPYEMTIQGKNQ
                     IVLKDVMIGEVWICSGQSNMQWTVRNSANAEEEIANSANPMIRLFTVPRQAKPEPQKD
                     VVGAWAVCGPETVADFSAVGYFFGRHLQKKLGIAVGLINTSYGGTPAEAWTNWNKLAC
                     MPELTPIVDRFRDALAKYPENLKRYEEALKAHREKARQAKERGETFTQRAPSPPMGPE
                     HPHSPAGLYNAMIAPLVPYAIRGAIWYQGESNAGRAYQYRTLFPAMIASWRDAWNQGD
                     FPFLFVQLAPYMKIETEPTESAWAELREAQLLTMLNCRNTAMAVITDVGEEDNIHPKK
                     KQPVGDRLAIAARALAYGEDIEYSGPIYERMEIQGNKVILYFTHVDGGLVCKGDKLTG
                     FTICGEDRKFVNAEAKIEGDTVVVWSDQVEKPVAVRFGWANYPVVNLWNKADLPASPF
                     RTDDFPGVTKDAQ"
     gene            complement(8454..11762)
                     /locus_tag="PLD05_01290"
     CDS             complement(8454..11762)
                     /locus_tag="PLD05_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOP76088.1"
                     /translation="MRSRAYACGLWLSVALGLIALQKQVVAQFESMPLAGAWRFQIDR
                     DNVGIEQKWWTNRLLDEIQLPGLLQAQGYGDPPGPQSQWLAGIGLKRADDPLFAPYFK
                     GKEFLSPFFLTPPRHYVGAAWYQRDVVIPETWKDCHITLTLERVHWESRVWIDDREVG
                     RQNSLAVPHVYDVSKYLSPGKHQITIRVDNSYLIPVGKSAHSVSDETQGNWNGIVGRM
                     ELQATPPVWIEDVQVFPNAKERLVDVKVTIGNMTGRAGQGQLRLSAHTLSREAVSPSE
                     RVHRVEPVVYPVSWSPEGANVTCRYHLGEGALLWDEFHPHIYALQVTLETAQTRDTGD
                     PAGLQHTRTVTFGLRDLEVRGTQFVLNGRPIFLRGTLECCIFPIHGYPPTDRESWRKV
                     FATARDYGLNHLRFHSWCPPQAAFEVADEMGFYLQVECSCWASFGDGGSQDAFVYEEA
                     ERIRRAYGNHPSFLLMVASNEPGGRNSNQFLAKWVETQIAADPRRCYSAGSGWPQLAV
                     NQFHVQPRTRLQNWGPLQLNKPPQTWDDYREYIEQLGKPTLSHEIGQWCAYPNVVSEP
                     EKYRGFFRGSNVEVFRDILKKKGMWDQAEDFIKASGRFQVALYKQEIETALRTPGMAG
                     FQLLDLHDFPGQGTAPVGVLDAFWESKGYCTADEYRRFCNSMVPLARLKKRIFTADET
                     LEFRIDLAHYGPRDLKSAVIEWELRQQSEVIARGELPPRDVPTGNVAEGDVLSVPLSE
                     IERLKVPTMLSLVARVKGTSWENDWNIWVYPRPAEPAQTEGITITRDPEEAWKLAEEG
                     QSVLLVPRPEIIAGDTLGTFQPIFWNRVTFPSQKVHMVGILCDPKHPALKSFPTAFHA
                     DWQWQELLDACKPMVLDGLPKNLRPIVQAIDDWCEARKLGLVWEAKVGTGRVLVCSID
                     ITSDLTQRVVARQLRASLLDYIRSTPVQPLIALQREEWETLWREPPLLQKLGAKASAD
                     SFEPGFEPACAIDGDPKTMWHSAWTPEPAELPHWFVIDLQQPLTITGLRVLPRQDGNP
                     NGQIAEFEVYVSRDGENWGEAVAKGTWDARPVERTIEFSSPVIARFIKFVARREIRGK
                     QFACIAELDIIVSQ"
     gene            12023..12430
                     /gene="gcvH"
                     /locus_tag="PLD05_01295"
     CDS             12023..12430
                     /gene="gcvH"
                     /locus_tag="PLD05_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOP76089.1"
                     /translation="MNPDELKYTKTHEWVAIEKTSDGKTLAVVGITDFAVQSLADLVF
                     IELPPVGKEVKAGEPLGQVESVKAVSDIYSPVTGRVVDANSHVAEHLEDLTADPFGRG
                     WLAKLEVTDPTSLSGLLDRASYQAQCAAEAEEH"
     gene            12661..14022
                     /gene="gcvPA"
                     /locus_tag="PLD05_01300"
     CDS             12661..14022
                     /gene="gcvPA"
                     /locus_tag="PLD05_01300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOP76090.1"
                     /translation="MAYLPQTEEEVRAMLAAIGVPDIDSLFASIPSDVRLNRPLQLPP
                     ALTELELTQHLEEVASQNQGTHQFTCFLGGGAYDHFIPAVVDFVASRSEFYTSYTPYQ
                     PEVSQGNLQVFFEYQTLITQLTGMDVSNASLYDGASALAEAVLMALGSQPGRRRVIVP
                     ENVHPEYRTVLATYLRYTSAEIVLVPTPKGIVEPEALSQALDAGSVACVILQQPNFFG
                     CVESAGRISEITHQAGAILIAVVDPISLGMLRRPGDYGADIVVAEGQPLGLPMAWGGP
                     YLGILACRETFLRRLPGRLVGQTVDRRGNRCWVLTLQTREQHIRREKATSNICTNQGL
                     MALRATVYLAAMGPGGLRRVAEACLQKAHRLYRQLIEKTGLKPLFSAPFFKEFALQAN
                     SADEVVELLEGAQHEGFFAGLPLGRWYPQYADALLIAVTEKRTEQGMDHLVETLSNLR
                     RRG"
     gene            14075..15550
                     /gene="gcvPB"
                     /locus_tag="PLD05_01305"
     CDS             14075..15550
                     /gene="gcvPB"
                     /locus_tag="PLD05_01305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOP76091.1"
                     /translation="MRNRQATQLIFELSQPGTGEEYIPAVNFTEDDIRSFLPAEELAT
                     ELPPLPEVSEPVLIRHFINLSQQNMSVDTHCYPLGSCTMKYNPKRNDRLAQLPGFQNI
                     HPYQPVETIQGLLKVLYSLQEMLAEIAGMDAVTLQPAAGAHGELTALLIAAAYFRHRG
                     EHRNTVLIPDVAHGTNPASAAMAGFRTVEVKSNAHGLVDLDDLQSKLNHQTAVFMITN
                     PNTLGLFETQIRGIAEMVHGVGGLVYMDGANMNAMMGIVRPGDCGADMMHFNPHKTFS
                     GPHGGGGPGAGPVAVKKDLADFLPVPVIVEKAPGQFALDEDRPLSIGKVRSFYGNIGV
                     LLRTYAYIRTQGAEGLHRVSQRAVLNANYLLSQLRNIIPVPHGSRCLHEFVASALKIK
                     TSRGVSAMDIAKRLLDYGFHAPTVYFPLIVKEALMIEPTETESKATLDALVEALRLIM
                     QEPPELVREAPHTTAISRPDEVQAARKPILKWTPSADGSNG"
     gene            15969..16424
                     /locus_tag="PLD05_01310"
     CDS             15969..16424
                     /locus_tag="PLD05_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76092.1"
                     /translation="MRSILGRLTRGFTLVELLVVIAIIGILIALLLPAVQAAREAARR
                     SQCTNNLKQIMLAFHNYHDIHKRFGPGLVKMGNWFAANNCPEGQCGTWSWGAFILPYL
                     EQTSLYQQLNPTMIPADAAIQPDAVYNIVRRPLPVFRGPSNTAGNEYRP"
     gene            16324..16974
                     /locus_tag="PLD05_01315"
     CDS             16324..16974
                     /locus_tag="PLD05_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76093.1"
                     /translation="MRQSNRTRFTTLFGALFPFFVVLQTRPETNTARKVPRIGFYGNV
                     DCTTGCADVFLANYVGSNNSYDLNREYTGTDQFNGFMGYGQTGDRPPRCTPLGDISDG
                     SSNTFAIGERAWRIGVRELRAAVPIMANGDTGNHSLQGQVYAMACGRWPLNCTFDQAC
                     DRGFSSMHAGGANFAMVDGSVRFISETIDHNPATNAVDSTYERLIAIADGQAVQLP"
     gene            17045..17461
                     /locus_tag="PLD05_01320"
     CDS             17045..17461
                     /locus_tag="PLD05_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOP76094.1"
                     /translation="MLARRALWFGVTLTCVLVAGCGSRGPALGRVEGTVTLDGVPLGG
                     ARIIFTPVDGGRASMAVTDGSGHYELEFAGGVKGAVVGKHKVSISTFEAGEKDDTGKL
                     VGHVPERVPPRYNKDTILEQEVKRGHQVIDFKLESR"
     gene            17533..18624
                     /locus_tag="PLD05_01325"
     CDS             17533..18624
                     /locus_tag="PLD05_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76095.1"
                     /translation="MMFRKAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCTN
                     NLKQLMLAVHTYVDTYQRLPARQSGRGHIQALEQRLRISGWVQLAPFYEQKSVYDMAM
                     QANNDPWTNGAWQTQIIPVLNCPSDSGDIPPRGGKRGSTSYAFCSGDNYETSVHSPTE
                     RSDAAQSVALMNTPIKNRGMFGRQDYNSLANVADGTSNTIALAERSRPRQDNDKGSVI
                     DDQSGDPASYIPLACRAWWSGQRYISGAPIFTGDTSPGYRWGDGLAFFAAISTILPPN
                     SAVCMLRGGGWTAVSGHFAPGIWTATSEHPGGVNVAMADGSVRFISDTIDCGNLGVVA
                     PSPTAGGISPFGVWGALGTKAGSETITGF"
     gene            18709..19215
                     /locus_tag="PLD05_01330"
     CDS             18709..19215
                     /locus_tag="PLD05_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOP76096.1"
                     /translation="MSQKVWPAAIVVSVVVVLSITVGCQKRSRGNIPKTVPVSGIVLL
                     DGQPVDGAMVVFVPTQSPGYGAYAMTDSSGRFQLKSSDDVMGAVPGDYLVQVTKLVVD
                     TGKPQFVVQEDVEHAQAAGGGGASPEPGASKNVLPEKYSNPKTSGIQITVPATGLDNV
                     EIKLSKGG"
     gene            19321..21141
                     /locus_tag="PLD05_01335"
     CDS             19321..21141
                     /locus_tag="PLD05_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76097.1"
                     /translation="MRALAKTAGSTLRSPSIGQTLGNVAKVWHPFAFWLAIYLVLSLL
                     AYGPALRYATASLPCGKPTLGVVALFNAWSIWWNADRLKAGLVGYWDAPIFWPEHGTF
                     AFSEPQPLSLIVAPVVWAAGPMMAYHAYFLLSLWLNGLLAARLLRRLGCGWWGAGLGG
                     ALVVWLPLVHRQPEMLQFVPLWPVLLMWTGAIRLLSSEARWHTIVEFAAGIVLTFMAC
                     VHYGVFSLLLLVPAFLVVLLPTTDHGLKTALQRSVRVVWRWVIGLMLAGVIVSAVYWP
                     MYRILRQHEFTRSPKVVQALSARPEQWLAVPPEAYGGVVDLSFGVDRPLHPGWLRLVL
                     AVVGAVVILTYHRKQRIARRGIMFLVVTAFLAVVASLGRNLALGEFRPWDALAIFVPG
                     IAQVRSPYRFAYLAQLAVLLLTGAGFDVAAVKLLNARTQGLRPIILAGRPRVIAVTLL
                     SGLMIGEVPPPRMILTMAPDLSHPPGWVTFLTQNRETNEPVLILDFPSGGQLVEYHRT
                     VSSMLWQTQFRLPLVNGYAGFFPRRWSNLASVWRVSPYSHEAFRLLQSLDVRILVRPS
                     DFPPPPEADLAPFHLELLMRDATGVEVYRLAWEDQSVPIR"
     gene            complement(21379..>21992)
                     /locus_tag="PLD05_01340"
     CDS             complement(21379..>21992)
                     /locus_tag="PLD05_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOP76098.1"
                     /translation="ATVGAFAALGLLGVQGRPVETLEDLRGLARAPAFDLRLIAWAIA
                     AFMFSLAGVPPLAGFWGKLAVFFSALDVGGSLGNNPAKTRLIILAIVGAINAAIAAAY
                     YLRVVAVLFFAPEEEKPQAEIRPASAAGLFATAICLILTVVIGVTPGRWIAHAHSAGA
                     SIRQMVAPTTAVRALTAVAGPASRSMTPNIRPSHPLVPHDAEK"
     assembly_gap    21993..22092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22093..22751)
                     /locus_tag="PLD05_01345"
     CDS             complement(<22093..22751)
                     /locus_tag="PLD05_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOP76099.1"
                     /translation="MFHLLITTDWLPEVFLGLAGVLCLVLSPFGLRRGWIYVLAGLGI
                     LAALMAVVLHTDYLARTPDLFQAFARELILFIGFLLLLSSGGAPVSYDGERVGTLILA
                     VLGAMFAVRAQDLIVLFVGLELLSLATYVLLYLDREGPHRREGATKYFYLSILASAIF
                     LFGVSYLYGLGRSVSYKALMIAGAEQGFSGPLASIALVFILAGLAFKIAAVPFHFYAP
                     DV"
     gene            complement(22726..24510)
                     /locus_tag="PLD05_01350"
     CDS             complement(22726..24510)
                     /locus_tag="PLD05_01350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOP76100.1"
                     /translation="MGTLLAISVFAPLAGVLIVGDSQKVARRVTLIVVLLTTALSGWI
                     AFRFPVGAQQFAVFEVPWWSRSDQTIGIRFALGLDGLSVWLYALTSLLMVTSVLVSWN
                     AIDRQAPTFYRLLLVLYTGMLGVFTARDLLLFYVFFEATLIPLFFLIGIWGHEERRHA
                     AIKFFLFTLAGSVLTFLGLLAVVIWTASYSGTGRMTFSIPELTTRLYELRSQARFPLS
                     VQLWIFWALFLGFAVKVPLVPLHTWLPLAHVEAPTAGSVLLAGILLKVGGYGFLRLVL
                     PIVPDAVRVLAPWLLTLCVVGIVYGAFTAFAQRDVKRLIAYSSVSHLGFCMLGVFCLS
                     PVGLHGGLLQMINHGLATGGLFAVVGMLYERYHTRTIEAFGGLARKLPILAFMTFVLT
                     FSSIGLPGLNGFVGEFLVLLAMFQRAWSHGGGELALAILATSGVVLGAWYMLTLVGRL
                     FFGPLNEPLASPVAERDGQAFAPHVGDEDWKTYHVSTGQHAIEDLNLREIMALAPLFV
                     FIVWIGIQPNYFLRRMTPAVRQIERSITLAVSPSDRDGQEVLGHIVMPQPSSPHAKLR
                     FAEQDRATARLALTENKDACGVSSVNHD"
     gene            complement(24613..26670)
                     /gene="nuoL"
                     /locus_tag="PLD05_01355"
     CDS             complement(24613..26670)
                     /gene="nuoL"
                     /locus_tag="PLD05_01355"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOP76101.1"
                     /translation="MTFLIILIAAMPLGAAILTAVLGPRLLREFSHVPAVTATAISFI
                     ASLALLLLVGQTLAQHSADGEQRVLERVVQLWSWADISPTGGSPQVPVAHGTVDFVRF
                     QIPVVLRVDPLTVFMLVTVTAVSLLVVIYSIGYMHDDPGYWRFFTYVSLFIFSMIMLV
                     SVSNFLLLYIFWEAVGLCSYLLIGFWYRKPAAAAAGKKAFLVNRIGDFGFALGIFLIW
                     SVYGTLNFHDTIDPETGEVYSAGVLGMARLVHPEQGLVHGAVATAICLLLFAGACGKS
                     AQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYMVARCMPLFEAAPYALPIVGIIAI
                     ITATMAAIIALTQTDLKRILAYSTISQLAYMFLGLSVGGLVGVAAAMFHLFTHAFFKA
                     LLFLGAGSVMHATGGVIDINEIGGLRRRLPWTHATFLVGSLALAGLCPFAGFFSKDEI
                     LHLVYEKGSASSLYFFLFVAAAVTAMLTAIYTFRAYFRTFFGEERVPAEAAGHLHESP
                     PVMVIPLVILAIASAVVGVAFGPGKVIEHFLAATPSLAAFAAHEVGAHHGGGAALIPW
                     LSTALTLGGIGWAALVVLAPGGVARPVIALTKAIGLYALSFHKFFFDELYTWIIVRPL
                     TWLAAVAAWIDRYFIDLLVDIIGHLPKLVGALLRSSHNGWIPWYGLGIVLGVLVLLAA
                     MFF"
     gene            complement(26676..27119)
                     /gene="nuoK"
                     /locus_tag="PLD05_01360"
     CDS             complement(26676..27119)
                     /gene="nuoK"
                     /locus_tag="PLD05_01360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOP76102.1"
                     /translation="MENTGLLMNYLLVGTCLFGLGVVGFLARRNLIVMFLSVELMLQG
                     VIVNFAGWDRYHGQATGQVFVLFIVGVTAAEAAVALALILTLVRAREGLDVLRLQKVR
                     EDIILPPVPDEGEVREAETPSWPRLPRVGRRPDIPQEVLEYRPSV"
     gene            complement(27150..27899)
                     /locus_tag="PLD05_01365"
     CDS             complement(27150..27899)
                     /locus_tag="PLD05_01365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOP76103.1"
                     /translation="MDAPLFVLILASIFTAGGLWWALPWGKARRRSVGWVLVAAGLAM
                     LSAMIPSVGSLLARSLLTLMSIVAVGSAVAMITSSRPLYAALWFGMAVLGTAGMLLFN
                     GAQFVAMAVIVVYIGAILVMFLFVLMLDDPKGTAGFNRQSWEPLTASVVGAVLIALTT
                     VTWLRHAATANGWSLAKETGKAIPDVVITRTVDLGNQLFLHYGVAVQALAIVLLLGLL
                     GAAVIIAHPLEPLLDFKSRAVPDETPTANES"
     gene            complement(27933..28517)
                     /locus_tag="PLD05_01370"
     CDS             complement(27933..28517)
                     /locus_tag="PLD05_01370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOP76104.1"
                     /translation="MKPDSSEIRWVDESPLNMGERSYLPLFWDGLTTTFRHLIGPKLT
                     VQFPEERHQFPDPLIYRGVHRLNRDEQGRVKCVACFLCATACPAHCIDIVAGPSPWPD
                     REKYPVVFNIDMLRCIFCGMCEEACPVDAIELTSLYDLSGKSREEMIFDKEKLLSIYD
                     QTKDQEPPGWLRGKREIEDLIELPQDDQDLTQDK"
     gene            complement(28576..29841)
                     /locus_tag="PLD05_01375"
     CDS             complement(28576..29841)
                     /locus_tag="PLD05_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOP76105.1"
                     /translation="MISFAVVTCIKIAVLLAALMTAAAYLVLVERWIAAWVQDRLGPN
                     RVGIPLTNIRIFGLGQPIADGLKLIFKEEFVPGHVDRILYNVAPVVTFVAAVAVYAVV
                     PLGAVDLGISGWAPVDLTVVPAFDAALIFLFAANGAAVYGIILAGWSSNNKYSLFGAM
                     RGAAQLIAYEIPLSLALIALACYAGTLRIDEIVLQQAESGVWNIVLHPLAFVVFFVAA
                     TAEAGRLPFDLPETEQELVGGFHTEYSGMRLMTFLVSEFLHMITAAVVIVLLFLGGWH
                     LWFLTGSEPVAGIGGAIIRIVVFVAKVLAVVFFFMWMRWSWPRFRFDQLMTLAWKPLI
                     SWGIVQVTILVVWYAWWEPLVVSQGWSSLPVLGLINWVTFILTWVITAAVAQPHYDNS
                     PRRDLVRLAQDMREGSPYLESPTVRPCDA"
     gene            complement(29930..31558)
                     /locus_tag="PLD05_01380"
     CDS             complement(29930..31558)
                     /locus_tag="PLD05_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOP76106.1"
                     /translation="MGIVIVDGKEVEIGAHERLNGIQVAARAGIEIPHYCWHPGLSVV
                     ASCRMCLVEVGTRDPKTGQIVMLPKLMPACNTQAKDGTVFVTNSEKVARARAMVEEDL
                     LLRHPIDCPICDKAGECRLQDYHFKFGQSERRAEIRPFTSRKRDIGDVFLFVDRCIMC
                     TRCVRFTREITGTSELLVVNRGAHEEIDVLPELPLNNKLSGNVVDLCPVGALADKDFL
                     YKQRVWYLRRHPSVCVLCATGCAIWVEENQDRVYRIKPRENPFVNRWWICNDGRYSYP
                     VIHHPNRLRGAWQRQNGNVSSINWAGAIGQMVDKVQQARQPALCLSPFLTNEEAYLAC
                     RWIKQLHPQSTTALGPIPRQGEDERFPGGFVISGEKCPNRLGVEGVAYWFEQPPLRFN
                     DLLARTAEGHFDVIVVLGGYIKPWIDAEQADALRRATALIVADVFPTPLSDRADLIFG
                     MAFFAEREGSYVNRQHRLQWSARAIRPPFGSRCLGSVFWELGGMSGLYQPREVLAEIA
                     RTIPYFSAASGEIPECGVDLRVNQLAEQTVSTGQ"
     gene            complement(31587..32975)
                     /gene="nuoF"
                     /locus_tag="PLD05_01385"
     CDS             complement(31587..32975)
                     /gene="nuoF"
                     /locus_tag="PLD05_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOP76107.1"
                     /translation="MMESFEPVLLANIQKPDSHTLRAYEASGGYATLRRVLKELSPAE
                     VVEEVRISGLRGRGGAGFPTGLKWSFLPADHPGPIYLCVNADESEPGTFCNRILLELD
                     PHQVLEGTIISAYATRATTAYVYLRYEYPLAYRRLSEAIREAYQAGYLGKNILGTGLD
                     LDMYIHRGAGAYICGEETGLIESIEGKRAWPRTKPPFPAVEGLFRKPTVVNNVETLAC
                     VRHIMERGAEWFRSIGVPPDPNDPRDPGSFGPKLFCVSGHVNRPGCFEAPLGIPCRRL
                     IEEYAGGVRGGRRAKAVVPGGLSTGLLSADELDTPMDFAGPIKAGCLGLGTGGVIVLD
                     ETYLIIDFLHNSCRFFAHESCGQCTPCREGTAWALRIVERIKAGRGRLVDLDLLLEIA
                     DNIGIIPGTTICGLADGAAWPIKTAIRKFRGELEDYIKRTNPKGWRSTRPVPAFSLEK
                     TDQANSMRTPVA"
     gene            complement(32972..33511)
                     /gene="nuoE"
                     /locus_tag="PLD05_01390"
     CDS             complement(32972..33511)
                     /gene="nuoE"
                     /locus_tag="PLD05_01390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOP76108.1"
                     /translation="MSQESKAQTVEGRGGALLPPEVIASIREYFPRYPNTRAVVLPAL
                     HLIQETLGYVPKSAVLELANVLGIPASDIEDTLSFYQFFKQDRPLGKYRIWVCRSLSC
                     ALCGGENLLDYLRERLNIEPGETTADGRFTLEAAECLGACEYAPAVLINETLYPTMTR
                     EKLESVLDELSKQEINGHG"
     gene            complement(33542..34762)
                     /gene="nuoD"
                     /locus_tag="PLD05_01395"
     CDS             complement(33542..34762)
                     /gene="nuoD"
                     /locus_tag="PLD05_01395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOP76109.1"
                     /translation="MPLQRSELSQATDEVQEYLWTMNFGPQHPATHTTLRLILKLEGE
                     RVVDADCDIGYLHSGFEKLAEHLNYNQYVTITDRMNYVSPMANNVAWHLAVEKLMGLE
                     VPPRCQYLRVIVAELARIADHLVCNAAVGLDTGAFTFFMYAFNPREMIYDIFEALCGA
                     RFTNSYTRVGGLMYDASPKAIELIREFLRKMPRALDDMDRLLTRNRVFVDRTKGIGVL
                     TKEEAINRGVTGPIARASGVTRDLRKDEPYLAYSDFDFQVCCAQGGDCYARYLVRMAE
                     MRESLKIIHQAVENLPSGPVHVDVGQRAIIPDKWQVHSTIEGLITHFEIIMQNRGFEV
                     PCEEVYCATESPNGELGFYIVGDGSPYAYRARCRPPSFVHFALFPHLIRGHMLGDVVA
                     VLGSLNIIAAELDR"
     gene            complement(34832..35329)
                     /locus_tag="PLD05_01400"
     CDS             complement(34832..35329)
                     /locus_tag="PLD05_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOP76110.1"
                     /translation="MVSSETIQAIQARFPSAEITEFRKQFRVCVPREIVHDVLRFLKD
                     ECGFTLIIDITCVDYLYYPKAKDRFGVVYIVGRPENADRLIVKTFVNDPEPTLPTVTD
                     LWEGARWLEREVWDMFGIRFEGHANLRRILLPEDFQDHPLRKDYPLQGRGERHNIPVV
                     TRDLG"
     gene            complement(35430..36050)
                     /locus_tag="PLD05_01405"
     CDS             complement(35430..36050)
                     /locus_tag="PLD05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOP76111.1"
                     /translation="MTDLPDNVVITKLDALVNWCRKNSLWPMPFATACCGIELMATGA
                     SRHDIARFGAEVFRFSPRQCDLMIVAGRVVMKMMPVLQRIWLQMHEPKWCISMGACAS
                     TGGVFDTYAVVQGIDRFIPVDMYVPGCPPRPEQLLQAIIDLQEKIQREGSAWGKDVER
                     RPHQPPRALLADPKNPRLLVAGPPIFAQDEAANKWDTGKRGESPDS"
     gene            complement(36103..36615)
                     /locus_tag="PLD05_01410"
     CDS             complement(36103..36615)
                     /locus_tag="PLD05_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOP76112.1"
                     /translation="MEFDAALVPFTLFLMALVGLSIGLIVLGHLCGPHRANPVKAMPY
                     ESGVDPFHDAQRRFHVRFHLVAIAFLVFDVELLFLYPWVVSLRQSSSWQSKAAALSSC
                     TETTGRSDAGVVVGLGSHESPSEQSGELATGALLQRSLQGILWPGLVFFVLLGLGYAY
                     DWRRGVFDWK"
     gene            complement(37135..39411)
                     /locus_tag="PLD05_01415"
     CDS             complement(37135..39411)
                     /locus_tag="PLD05_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76113.1"
                     /translation="MGSQGKPFWLLYAAYDRWSYRDTPAARFKFRWAHARFAGLARLF
                     VLFLVLLGIYHSAACAAQSFGEGATSAQHLVLAEVALSDPADATQVYAGKPYVWLGKY
                     IVSIQINGPLVDGLVLQLSAGAKNDRRGLAYEWIEQGKTIESGRTTYGGCDGFQDLAF
                     ELPARSGDSLEFRLSGASDKAAFFSHAALICQGGKTEGSLPKFTLRVNLLPVTVGGPV
                     DGGFADRYPEMQAIWRDPQALSPDEVPEGSAEWAFRCAARNGRLANEMFFCANKYVQG
                     WLEQADPKTGLIPRNLTQSRDIWNPEDAAADNYPFMVLTSYLTERGLFDGRMLAMLET
                     ETRLTSRLGSIPDAWSFSKQGFVRPEPQLGALIFGGSEYVKDGLLPLTEFLGPSPWLD
                     RMLAIVDDIWKVAPVETPWGAIPSENVEVNGEQLQILSRLFWLTGNRKYLDWAKRLGD
                     YYLLGSHHPTRNMQNLRLRDHGCEIVSGLCELYVAVSFADRAKAEEYREPIHAMCDRI
                     LEVGRDARGMLYNSINPLTGEHDSNICDTWGYNYNGIYSVYLVDKTPGYREAVRFVLS
                     QLESQVGDYHWGSADEYADSIESAINLYNREPDSSAAAWIDRKIHYMWRPQQPSGIIE
                     GWHGDGNVARTGIMYAFWKTQGLRVEPWREDVRLGAVLHEGRLYVTLTVSKPWEGRLV
                     FDRPRHRHYFHLPIDYPRINQFPEWFTIDDCEEVRLLNADEKEVCLATAEEAWNGVPI
                     RMDQAGEMRWIIVKRSKK"
     gene            39863..40729
                     /gene="lpxA"
                     /locus_tag="PLD05_01420"
     CDS             39863..40729
                     /gene="lpxA"
                     /locus_tag="PLD05_01420"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOP76114.1"
                     /translation="MGVRIHPTAVVNSFAALGTDVEIGPFCVVEADTIIGDGCRLESR
                     AVVKSGTTLGENNWVFEGAVLGGMPQHVHMPERPGRVIIGSGNVIRENVTIHRALDEE
                     DATLIGDNNLFMANVHIAHDCRVGNNTIFANNAMLAGHVTVGDRAYVSGAVAVHQFCR
                     IGAYAMVGGQAHIVKDVPPYVTVDGLSSYVVGLNQIGLRRAGFSTEAIAELKQAYRVI
                     YRSGLSWKDILEQLRTKFSTGPASLFYQFLSTTTRGIIQERRTPQGVTLKLRRADDED
                     SDTAEWRVRITG"
     gene            complement(40897..>42824)
                     /locus_tag="PLD05_01425"
     CDS             complement(40897..>42824)
                     /locus_tag="PLD05_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76115.1"
                     /translation="RIWLARQRWGVRWCLVWSILLAAWICLSAVTSDTNLNRDGTLGA
                     TLFYSLLGGAVLYAAGQLAGMFAPGILHALILAAGLTLAVAMPVMFFPVGPDEIIPGG
                     SFAITVAILLAAFFWQSRRSLRSWLEERAAGGFRDRLSSPILWGLVVTFLILPWTRVM
                     FLPRTDVESLRNMEAAAQVRLQQWMEPQPESLQGFAATMEVARRVKFSGPLSSLAVGR
                     RTWSYELGEDPGQRFLESESAVDFFRVLGELSKQPYADPLVDALRHGSPWERLDVNSQ
                     AGTLGLELQMITIPALENLARAAVRAGKIQESRSLLIAALALRFRAERTYPAAFGNFF
                     VSGRDLAQWSRWADAAWEWSRSEGQTPQSLREMLQDIQDVVVRERVPLEVRTLLLGTY
                     WRGPFLRDAPIGMGREGPRFAWWSDELWFLPIAVSWVPGEWQLHQARLDRILAEYLPR
                     AVEYDERGVFPWQFRRRLESSKPVSLWRGVGWNAAASIRRSLEDAFFSRALGDLHVQA
                     TCLRLALRMYKLEHGRYPGRLEELVPRYLPRIPRVPGSGEDFLLSYPRRIRKRARRRR
                     TRFWMPGFMSGVPAVTSSCWSWTRQKTVMGKNVSSEITAGRTRQNFLRMSSLGLHRRN
                     RAGHASVLRRVHPGLSA"
BASE COUNT         9845 a        12342 c        11556 g         8981 t
ORIGIN      
        1 cgcgaacgtt tgatcagcgt cggggccgat ctcgtaattc cagactaccg ggattggcaa
       61 acgttgatcg cttacctttg gggagagatc acggcgcctt gtgtgccgcg agagagtctt
      121 gggaaagaat gtgtctgaaa acttgagagg ccaatgggaa tatgggctat ccgcaatttg
      181 atcgatcgcg gttaatcctg cggcctttgg ccgagcgaac tcacgacatc gatcgcagca
      241 tccttttaga cccaacaacg gtacagtcgt catttcacca cccggcaatc ccgacattgg
      301 cggtccggat tctcgaggcc cgaaagcggg gcgcagccgt tatcgccttc tgcggagcgc
      361 acgtcttgag aacgggaaac ggtccccttt tgattgacct tatggaaaag gggttactga
      421 cccacttggc tcttaatggg gcgggggcca ttcacgattt tgaattggcc ctgattgggg
      481 caacgtgtga aagtgttgct cgttatattc ggacgggcga atttggcttg tggcaagaga
      541 caggccgaat caacgacgcc gctatcatcg ccgcaagaga gcaaataggg ttcggcgaag
      601 ccctcgggcg gatgatcgaa accgaacggt tcccctaccg cgagaccagc gtgttggcgg
      661 caggctatcg gctgcgggtg ccggtcaccg ttcacgtcgc catcggccaa gacattgtcc
      721 acgagcatcc gaatttcgat ccggtggcca tcggccaggc cacttacacg gatttcctca
      781 ttttcacgca cagtgtggcc aatctcgagg gcggggtatt cctaaatatt ggctcggctg
      841 tggcaggtcc tgaggtttat ctcaaggcct tgagcatggc ccggaatgtc gcgcggcaag
      901 agggacgcga gattcgcgac ttaacgacgg ccgtttttga cctggtggcc atcgggagtg
      961 attggcgtac tgaggcccca aagacggatc cccggtatta ctatcgcccc ttcaagacgg
     1021 tccttgtccg cacggttgcg gatggtggca cgagtttcta tgtccaagga gaccaccgcg
     1081 agacggttcc cgccttgtat cgcacactca cgactttcca gaaggagctc gacacactcg
     1141 acacaacagt ggagtggcgg tcacaattcg aacgtcgggg agccagtcgg atgaagcagg
     1201 ttgaacgatt tgcgggattt aggaaagacc gcctggaaga gattctttcc cgcttcaaaa
     1261 atcttcgcgt cgccgttgtg ggtgactttt ttctcgataa gtacctcgac gtcgatcccc
     1321 gattggcaga gacgagctta gaaaccggta agctagcaca tcaggtggtg cgggtccgcc
     1381 acagcccggg ggccgcagga actattgttt gcaatttagc ggctttggga atcggcacca
     1441 tttactgcgt gggattgatc ggggacgacg gtgagggtta cgagttgcgg cgagacttgg
     1501 tcaacttagg ctgtcacatc gagcatctcc acgtttgccc cgatgctatg acccccacgt
     1561 atctcaagcc gcgggacatt accgatcctt cgctggccgg ggagcattct cggtatgatt
     1621 tcaagaatcg atctcagacg ccgcctgaac ttcgccaaaa gattgccaat tcgctttcga
     1681 cactcctgcc ccaggttgat gccgtcattg tcttggatca agtcgaagaa gcagactgcg
     1741 gcgtggtcac gaaggaccta agagaactga ttgcggaact ggccccttgt catcctggtg
     1801 tgatcttttg ggcagacagc cggcaaagta tacgagagtt tcgccacatt catcttaagt
     1861 gtaaccagtt cgaattactt ggaaacgcgc tcgacccgtg gcggcccgtg cccgactggg
     1921 atattctcat cccggccatg gaggagtttc gcaaggttaa taatgcacct attgtggtaa
     1981 cgctggggaa agacggaatg ctggtgagcg atcctgctcc cacctggatt cctggggtga
     2041 gggtttccga accgacagat cccacgggcg ccggcgatag tgccacggcc ggcgctgtgg
     2101 cgtctctggc cgccggtgca acgcttcccg aggccgcact cgtggggaat ctcgtcgcat
     2161 ctattacgat ccagcagttg gcaaccacgg gcaccgcccg gccggaagag ctccctcccc
     2221 gtttggaaat gtggctcgag cagcaagcac aaacataacg cccacgttac cttgagattt
     2281 acatcgactg ctgcgttgct acggtatcac cacatgaatt ttgccaattt ggtctgcggc
     2341 aggactttat agcccatgtc ggattggaag cctaccatca cccatcacct gaccaaatgg
     2401 ctgcggcgaa acaaacccgc catacgggca ctgttggggg attttttgca agcgatcgaa
     2461 atagaacagt tcttagcact cggcacatcg caaaacacgt tctatgacag caccaaagag
     2521 gatgatctta cccaaagctg ggccgccgaa tggggacaag cggaccacat tccacttgcc
     2581 gcatttcccg tgattgttgc ccagttttcc agttggctca ttacggcggt tgccgtccat
     2641 ggtctctccc attggaacgt gccagacctt cctaaatcgc ccgatctatt cgccaacgaa
     2701 gtaatgcggt ggctggatga agattgcgca cgcagggccg gttcgtctgg tttgtggcac
     2761 ttacttgact ggcttggcat tcgactaccg tgcatcgcca accagggcga tgcgcatcgt
     2821 tttttcaaac acctcgccga gtgggcaggg caattctttg agcaatggca tcagcaaacg
     2881 gatacctggt gtgacttatt ccagcccctg cacacggtgc ttgtccccaa gaagattcgt
     2941 ttacgaatgg gggagtttta cacgcccgat tggttggcgg agcacatcct ggccgaactc
     3001 gactacccgg gatcggatgc ctcgcgcctg tttgatccca catgcggttc gggcgtcttc
     3061 ttgacggcag ccgtgcgacg tctgattcct catttgctcg caccccacaa tgggtggaaa
     3121 acgggtgggc gtagtaccgg ttcacaatcc cctcaaaccc gggcatgtcc agaaagctta
     3181 gccctcgaag tcgttcgcgg ggttcttaag gatcaaatcg tgggcatgga cacaaatcca
     3241 ctggccgtgg tggctgccca ggcaaatttg ctgttggcca caattcacac cctgtggcgg
     3301 atttgtccgg caatgcgctt gccgctggat gcggtactcg accatcccag ccccatccgg
     3361 tgtggtgacc ttctcgattc ctcatgggag actcccacag cgaatgcacg acctatgggt
     3421 ccaggcgcag aacggcaaac taagccgcag attcagccac agtttcattt cctggtggga
     3481 aatccgccgt ggcttctttg ggacaatctg acggcgtcga tgagagaacg gctcgcacct
     3541 ttgtgtcagg aattgggatt gtttgaccta tctgggcgag aggcacgaca tggcggggcc
     3601 aaacgcgacg ttggcgtggc gctcgtgtgg atggcgacac agtatttgct gcccggtggc
     3661 aagatgggtg tggtgcttcc tgccacactc ttccgcgggg gaaatgctgg ccgcagcttc
     3721 agacgattca ctgtagggcc tgagacgccg ttgcgggtcc ttcaggtaga agacctttcc
     3781 ggactgccgg tgtttccggg aaccatccgg agaaccgtcc tattctttgc agaaaaaggt
     3841 ttccccacgc gctatcctgt tccgtattat gtctggcata aagaagctgg acagcgagag
     3901 atggacaacg gcaggctaca tgcttcccgc accaaacttg tggcagtacc ctcttgccct
     3961 gatgacaccg cggccccttg gtcgatcaca acagagagcg ctcgtaacat ttggcagaaa
     4021 gtggcggggc cctcggagta ctccgcctac ctgggtgcga ataccgccgg ggctaatggc
     4081 gtgttttggc tggaaatcat cggccacgag tgcggacttg ccgtggtgcg aaactgtgct
     4141 gaatccggtc gccgagcaac gccacacttc caaggcccct tggagccaga tttgctctat
     4201 ccccttttgc gatggaaaga tgtgatcccg gcaggggttg cctccacggt cattctgctg
     4261 gtacaagatc ccctgactcg aaaggccatt ccagaggagt tgctcaaagc ccgctacccg
     4321 cttacttatg cctatcttag ccaatttcga gacatcctcg aagctcgtgc tgcacggcga
     4381 aagcttcacc gcaaggaacc ttggtattcg cagtacaatg tgggtcatta caccatggcc
     4441 ccacataagg tcgtttggcg gagaatggat tggaaatttc gcgccgtgtt ggtcggtccg
     4501 caaccactgg gagagttagg ggtccgaccg gtcgttcccc aagagacctg ttgtttcatt
     4561 ccggtggatt cggttgagga aggggcgtat cttctggcga ttcttaatag tcgtcttgtc
     4621 cgggaaattg tccaagcgtc ggccctgccc ggctcacgtg gctttgcctc gccgcggatt
     4681 cttcggacgc tcgccattcc ccgttttgac ccccgagtcg atcttcaccg ccgcctggcg
     4741 gagatcggtc aaaaggcgtc ttccaccggt caagtttcct gcgacaagtg gcttgaagca
     4801 aacaatctca cggactggga ccgttgcctg gccgaactct acggcctttc gccgagtgac
     4861 tacgtggcat tgtgcgaaaa gagcgtgtga aactacaaag ggcaagtttt ttcacggtta
     4921 gggagatgaa ccattgattc cgaggcggct cagccatcgt ccgcggcttg gtttgttctt
     4981 ttcttcttcc ggggacttgg acggtcccaa ggccgccagt ttttcttcga gttcgcgctg
     5041 tttttcctca aggaccgctc gttcgcgcgc gatttttgcg cgttctagcg aaatctctaa
     5101 ctcggccttg cggagctttt ccttccactc ctcttccagt tgccggagat gctcccgctg
     5161 ctcccgcaga acctcatcct gatcgaagag ccgctcgatg gctgcggcac ctactgccac
     5221 cgaaccaatg ctggtcgatt gatcctcgag taaccgccgc aactcggcaa tctcacggtc
     5281 tttctcggcc agcgcggcct ccgttttctt gataacatct tgaatccgta tccgttcggc
     5341 cgcacgttcg gggttggaat cctcttgcgc ctctgcttcg agagctgcga gaatacgttg
     5401 tttttcggcc tcccaatcga gaatccgtcc gtcggacgag gtcaccaccg gcttggtccc
     5461 cgattgttcg atctgcgcca gttttttctc caattcggcg tttttctgcc tgagttcacg
     5521 gatttcgaca atggccattt cgtagcgctg ctgaaggtcc gaagatgcct gcgggtctga
     5581 ttgtctccgt agcttttcca gttcttcttc caattcacta caccgaatga ccaaggcatt
     5641 gaaggcagac tctcgatcat tccactgggc ccgccagcga tctttttcgc tttcgaactc
     5701 cgcccgcagt tgggcctcgc gccgctgcca ctgctcggcg tccgtttgtt tttctgattg
     5761 actgatggcc aactgttgct cgaggtgcgc cttgatcgat ctaagctctg ccatttcccg
     5821 ccgcatggca gccaattcgg ccgtcagttg cgcatacagt tggctccggt tctcattttc
     5881 cgttcggagg gattccccac gttcctggat gctacgcacc gccgaatcaa gcgctgaaag
     5941 aagcggcaag attttttccg atgatttcga ttctctctga gaaagctccg cccgcaaggc
     6001 ctcgaccgtt ttagccaacc ccccaagata gtcccccaat tcctgctcgg cgaggcggaa
     6061 cctttcctgc tggcgcgaaa ggacttcccg gcagcgtgcg cggtgctcat gcaagagtcg
     6121 aacgacttgt tcacccgccg ccaaagcctc agaagatgat tcggaggtaa tcatgagcga
     6181 ttttgtctgt cttttggctt tcgttgagtt tcttcggact agggtcgcac cttctggctc
     6241 gattaacggg gagaacgagc ttttttccga tgggactact gcccagctcg ttatcacaca
     6301 gatccctctt tcataggtct aggttgcagg attggacgag tcaaattgtc atgccaatta
     6361 tgcagattgc caaaaacctg ccgaatttgc cggataaaaa caaaaacagg ggagaaacga
     6421 tcgtttctcc cccctgttga agtatcggga atattcgttg caagtgaccg ttcacgggga
     6481 gcggaggatt ttctcgaagg atgcccttca tgccagcggc tgtcgtcctc tgccatctgg
     6541 ggagggttat catcctcggt gaccaaatca ccggagggcg aggacggcgt ccctccgtct
     6601 tcttcgccaa cccgaggaac tccttaacgc gcaacccctt gagtcccaag aagttgctaa
     6661 tcttccccgt gaacggttat cgttgcaacg atctgagcgc tactactgag catcttttgt
     6721 cacacctgga aagtcatccg tgcggaaagg tgaagccggc agatcggcct tgttccacaa
     6781 attgaccacc ggataattgg cccagccgaa acgcaccgcc accggtttct cgacctggtc
     6841 gctccacacg acgacggtgt caccttcgat cttggcctcg gcgttgacga actttcggtc
     6901 ttcgccacag atggtgaagc ccgtcaactt atctcctttg caaacaaggc caccatccac
     6961 gtgagtaaag tagaggatca ccttgtttcc ctggatttcc atccgttcgt agatcgggcc
     7021 ggaatattca atgtcctcac cataggcaag tgcccgcgcc gcaatcgcca aacggtcacc
     7081 aaccggctgc tttttcttgg ggtggatgtt atcttcctca ccgacgtcgg tgatgacggc
     7141 catcgcggtg ttgcggcaat tgagcatcgt caacagttgg gcttcacgca actctgccca
     7201 agcactctcc gttggttccg tttcgatctt catgtaaggg gcaagctgca caaaaaggaa
     7261 gggaaaatcg ccctggttcc acgcgtcacg ccaggacgcg atcatcgcgg ggaacaaggt
     7321 ccgatactgg taggcccggc cggcgttcga ctccccctga taccaaatgg ccccgcggat
     7381 ggcgtatggg acaagaggtg caatcatggc gttgtacagc ccagcggggc tgtgcggatg
     7441 ctcgggcccc atcggtgggc tgggagcccg ctgcgtgaat gtctcgcctc gctccttggc
     7501 ttgccgggcc ttttcacgat gggccttgag cgcttcttcg tagcgcttga gattctccgg
     7561 gtatttcgcc aaggcatcgc ggaagcgatc aacgatgggc gttaactccg gcatgcaggc
     7621 aagtttgttc caatttgtcc aagcctcggc aggtgttccg ccgtaagaag tattgatcaa
     7681 tcccacggca atgccaagtt tcttttgaag atgccggcca aagaaatacc ccacagcgga
     7741 gaaatcggcg acagtttccg ggccacacac agcccacgcc ccaacgacgt ccttttgggg
     7801 ttcaggcttc gcttgacgcg gtactgtaaa gagacgaatc atgggattgg ccgagttagc
     7861 gatctcttcc tcggcgttgg cggaattcct taccgtccac tgcatattgg actgcccact
     7921 gcaaatccac acctcaccga tcatcacgtc ttttaaaacg atctggtttt ttccttgaat
     7981 tgtcatctca taaggaccac cggccggcaa cggttgaagt ttcaccatcc agtcgccgtt
     8041 gttatccgcc ttggtcgtga ccttttgatc ccccagccta acggttactt cctcaccggg
     8101 atctgcggta ccccagaccg ggattgtcat ctgttgctgt aaaaccgcat ggtcgcaaaa
     8161 gagcgccacc ggtttaacgt cggccaacag actgccagct acaatcaaga caagccacgt
     8221 tgccgacagt ccccacatca aacgccatct gttgctgcta atcctcatgt catcctcctc
     8281 gctccatgtg ggaaaaaaca aaacgacgtg ccttcacccc aaaaacctgt gattattctg
     8341 acgccaatca gagtaccccg caagcacccg caacgtgcaa cgcctcattt cgtctcacta
     8401 ccgatgatgc ggtgctgagg gcacaccact atccgatgat ctcggatttc tcgttattgg
     8461 ctaacaatta tgtccaactc ggcaatgcaa gcgaattgtt ttccccggat ttcccggcga
     8521 gcaacaaact tgataaatcg ggcgattacg gggctggaaa actcaatggt tcgttcaacc
     8581 ggtcgggcgt cccatgtccc cttcgccacg gcctcacccc agttttcgcc atctcggctg
     8641 acgtaaacct caaactcggc gatctggccg ttggggttcc catcttgccg tgggaggaca
     8701 cgtaagcccg ttatcgtcag cggttgttgc aaatcgatca cgaaccagtg aggcaactcg
     8761 gccggttctg gcgtccacgc cgaatgccac atggtcttgg gatcgccgtc aatagcacag
     8821 gctggttcaa accccggctc aaagctgtcg gcactggcct tggcacccag tttctgcaat
     8881 agcggtggtt ctcgccaaag cgtttcccac tcctcacgct gcagtgcaat gagaggttga
     8941 accggtgtgg atcgaatgta atcaagtaaa ctggcccgaa gctgacgagc gaccacacgc
     9001 tgcgtcaaat cacttgtgat gtcgatcgaa cagacgagca cgcggcccgt tcccaccttg
     9061 gcctcccaaa cgagtccgag ttttcgtgcc tcgcaccagt catcaatggc ctggacgata
     9121 ggccgcaggt tctttggcaa gccatcaagc accatcggct tgcacgcatc gagtaactct
     9181 tgccattgcc aatccgcgtg aaaagccgtg ggaaaacttt tcaatgcggg gtgcttcgga
     9241 tcacacagaa tccccaccat gtgaaccttc tgcgagggga atgtgacacg gttccaaaag
     9301 atcggctgaa atgtccccaa cgtatcaccg gcaatgatct ccggtcgtgg taccagcaag
     9361 accgattgcc cctcttcagc cagcttccag gcctcttcag ggtcacgggt aatagtaatc
     9421 ccctcggtct gtgctggttc tgcaggccgt ggataaaccc aaatgttcca atcattttcc
     9481 caggatgtcc ctttgactcg tgcaacaagc gacagcatgg tcgggacttt gagtctttcg
     9541 atttcgctaa gaggcacgct taagacgtca ccttccgcca cattaccggt cggaacatcg
     9601 cgcggtggca gctctccccg ggcgatcacc tccgattgct gacggagttc ccactcgatg
     9661 acagcgctct tcaagtcccg agggccataa tgggccaaat cgatccggaa ctccagcgtc
     9721 tcgtccgcgg tgaaaatccg ctttttgagc cgggcgagag gcaccatact gttgcaaaag
     9781 cgcctgtatt catcggctgt gcaataaccc ttgctttccc aaaaggcatc gagcacaccg
     9841 acgggagccg ttccctgtcc cggaaagtcg tgcagatcaa ggagctgaaa acctgccatg
     9901 cccggcgttc gcagggccgt ttcaatctcc tgcttgtata aggctacttg aaatcgcccc
     9961 gatgccttga tgaagtcctc ggcctgatcc cacataccct tcttcttcag aatgtcgcgg
    10021 aagacctcca cgttggagcc acggaagaag ccccgatact tttccggctc tgaaacgaca
    10081 ttgggatagg cacaccactg accgatttca tgactgaggg tgggtttccc caattgttct
    10141 atgtattcgc gatagtcatc ccaggtttgc ggtggcttat tgagttggag cggtccccag
    10201 ttttgtaatc gtgtccgagg ctggacgtgg aactgattga ctgcaagctg tggccaaccg
    10261 gaccccgccg aataacaacg ccgtggatcg gccgcaatct gcgtctccac ccatttggcc
    10321 aagaactgat ttgaattccg tccgcccggt tcattgctcg ccaccataag cagaaatgac
    10381 ggatgattgc cataagccct gcgaatccgc tcggcctctt cataaacaaa ggcatcttgc
    10441 gacccgccat ccccaaacga agcccagcac gagcattcca cctgaagata gaaacccatt
    10501 tcgtcggcaa cctcaaatgc cgcctggggt gggcaccagg aatgaaagcg caggtggttc
    10561 aagccgtaat cacgcgccgt ggcaaaaacc tttcgccatg attcgcggtc tgtcggtgga
    10621 tacccgtgga tcggaaagat acagcattcc aaggttccgc gcagaaagat cggacgaccg
    10681 ttgagaacga attgcgtccc ccgaacctcc aaatcgcgaa ggccaaaggt caccgtccgc
    10741 gtgtgctgaa ggcccgccgg atcccctgtg tcccgagtct gagctgtctc caacgttacc
    10801 tggagtgcgt agatatgagg atggaattca tcccatagga gagccccctc gcccagatgg
    10861 taacgacagg tcacgtttgc tccttcgggg gaccaactga cgggatagac cactggttcc
    10921 acacgatgga ccctttcaga aggagaaacc gcctcccggc tcagagtgtg cgcactcagc
    10981 cgcaactggc cttgcccggc gcgacccgtc atgttgccaa tagtgacttt cacgtccacc
    11041 aaacgttcct tcgcattggg aaagacttgc acgtcttcaa tccacacagg cggtgttgcc
    11101 tgcagctcca tcctgccgac gatgccgttc cagtttccct gggtctcatc cgagacactg
    11161 tgggcgctct ttcccacggg aataaggtag ctattgtcca cgcgaatcgt gatctggtgc
    11221 ttccccggcg acaagtattt cgacacgtca tagacgtgcg gtacggcaag ggaattctga
    11281 cggccgactt ctcgatcatc aatccaaacg cggctttccc agtggacgcg ctccagcgtg
    11341 agggtaatgt ggcaatcttt ccaggtttcg ggaattacca cgtcgcgctg gtaccatgcc
    11401 gcgcccacat aatggcgcgg tggtgtcaaa aagaagggcg agagaaattc tttccctttg
    11461 aagtatgggg caaagagggg atcgtcggcc cgcttcaaac cgattcccgc cagccactga
    11521 ctttgtggac ccggcggatc gccgtagccc tgagcttgta aaagccctgg gagctgaatc
    11581 tcatctagta ggcgattggt ccaccacttt tgctcaatac cgacgttatc ccggtcaatc
    11641 tgaaagcgcc atgcgcccgc taagggcatg gattcaaatt gggcaacgac ctgtttttgc
    11701 agggcgataa gcccaagcgc gacagaaagc cacaaaccac atgcataagc tcggctccgc
    11761 atccttaagt ctcctcacga tgaaccacgt tctacgaatc gccaagagtt atgcaatcta
    11821 atcaaatcgt taggaaaaag ccaccatccg gcaagaatgt cccgtgacaa tccgggggaa
    11881 ctactgtgac aatcatctgt gttataaaat gcattattcc gatttgccgg gcttgcatgg
    11941 tgttctcaag tcgccaattg cgagcggcac tcggaggcta tcttcaccag ggcaagattt
    12001 tccaatcgag tgggggtttc gcgtgaatcc ggacgaattg aagtacacca agacacacga
    12061 atgggtcgcc attgagaaaa catccgatgg caagacgctc gcggtggtgg ggatcaccga
    12121 tttcgccgtg caatctttgg cggacctcgt cttcatcgag ctacctccgg tgggcaagga
    12181 ggttaaggcg ggggagccac tcggtcaggt cgaatcggtc aaggccgtca gcgatattta
    12241 cagccctgtg accgggcggg tcgttgatgc caattcccac gttgcggagc acctcgagga
    12301 ccttacggca gatcccttcg gacgaggttg gctggccaaa ctcgaggtca ccgatccgac
    12361 aagcctatca ggccttctcg accgggccag ttatcaggcc caatgtgcgg ccgaggccga
    12421 agaacactga gcgtggtcac gtacgggcag aacatcttgc cggacttgcc agggttttct
    12481 aaggctttga ttttttcggg gcacgatggg gaatacaaag gtgggcatta cgcatggctc
    12541 gccgaccaag gtggtcggtg agtatgattc tttgacaaat gatgttcggt tgttggccga
    12601 tcgttatctc gcggcagatg gcgatggccg tcgctgacgt ctttttaggg ttaatgagac
    12661 atggcgtatt taccgcagac cgaggaagaa gttcgggcaa tgcttgcggc aattggcgtg
    12721 cccgatatcg acagcctctt cgcctctatc ccgtccgatg tgcgactcaa tcgcccgctg
    12781 cagcttcccc cggcgctcac cgagctggaa ttgacgcaac acttggagga agttgcctcc
    12841 cagaatcaag ggacgcatca attcacgtgt tttctgggag gtggagctta tgaccacttt
    12901 atcccggccg tggtcgattt tgtggcctcg cggagcgaat tctacacgtc ttatacccct
    12961 tatcagccgg aggtcagtca aggcaaccta caggtctttt tcgaatacca aacactgatc
    13021 actcagttga cggggatgga cgtttccaat gccagcctct atgacggggc cagcgccttg
    13081 gctgaagccg tgcttatggc ccttgggagc cagcctggac gacggcgggt gattgtcccc
    13141 gaaaatgtcc atcccgagta ccgcacagtc cttgcgacgt acctgcgtta cacgtcggcc
    13201 gagatcgtcc tcgtgccgac gccaaaggga atcgtggaac cggaagccct ttcccaggcg
    13261 ttggacgcgg gctcggtggc atgcgttatc ctgcagcagc cgaacttctt tggctgcgtg
    13321 gaatcggcag gtcgcatttc cgagatcacg catcaagcgg gggccatcct gatcgctgtg
    13381 gttgatccga tcagcttggg catgcttcgc cgaccaggcg actacggggc cgacattgtg
    13441 gtggccgaag gacagccact cggtttgccc atggcatggg gaggacccta tctaggaatt
    13501 ctcgcatgtc gcgaaacatt tctccgccgc ctgcccggac ggcttgtcgg ccaaacggtt
    13561 gatcgccgcg gcaatcgctg ttgggtcctc actctacaaa ctcgcgaaca gcatatccgc
    13621 cgagaaaagg cgacaagtaa catctgcacc aatcaagggc tgatggccct gcgggcaacc
    13681 gtgtatttgg cggccatggg gcctggcggt cttcggcgtg tggccgaggc ctgtttgcaa
    13741 aaggcccatc gattgtatcg ccaattaatc gagaagacgg ggctcaagcc gttgttttcg
    13801 gcaccctttt tcaaggagtt cgccctgcag gccaattccg ccgacgaggt tgttgaactt
    13861 cttgaggggg cgcaacatga aggattcttt gccggtctgc ccctgggacg gtggtatcct
    13921 caatatgcag acgccttgct catcgccgtc accgaaaaac gcaccgaaca gggaatggat
    13981 cacctggtcg aaactctgag taatctgcgg aggagaggct gacaccgaaa gcgaggcgat
    14041 gttgtcatcc attgtatagt tgtgcaggtc aatcatgcga aaccgacaag cgacgcaact
    14101 gatcttcgag ctttctcagc ccggaacggg cgaagagtac atccccgcag ttaatttcac
    14161 cgaggatgat atccgatcat tcctcccggc cgaggagttg gctaccgagt tgccccccct
    14221 gcccgaagtc agcgaacctg tgctcattcg gcatttcatc aatctttccc agcagaacat
    14281 gtcggtcgat acgcattgtt atccgctggg ctcctgtact atgaagtaca accccaagcg
    14341 caacgaccga ctagcccagt tgccgggttt tcagaatatt catccttatc agccggtcga
    14401 aactatccag ggcctcttga aggtcctgta cagtcttcag gaaatgcttg ccgaaattgc
    14461 cgggatggac gcggtaacgc ttcaaccggc agcgggggcc catggggaac ttaccgccct
    14521 gttgatcgcg gcggcctatt tccggcaccg tggcgaacac cgcaatacag tcctgatccc
    14581 tgatgtggcc cacggtacca atcccgccag tgcggcgatg gccggatttc gcaccgtgga
    14641 ggtcaaaagc aatgcccatg ggttggtcga tctggacgat ctacagagca agctcaacca
    14701 tcaaacggcc gtctttatga tcaccaatcc caacacactg gggttgtttg agacacaaat
    14761 tcgaggcatt gccgagatgg tccacggagt gggtggcctg gtatatatgg acggggccaa
    14821 tatgaatgca atgatgggaa ttgtccgacc cggcgattgt ggcgccgata tgatgcattt
    14881 caacccccac aagacattca gtggaccaca tgggggaggc ggtcccgggg caggcccggt
    14941 cgccgtcaag aaagacctgg ccgattttct ccctgtgccg gtgatcgtgg agaaggcccc
    15001 cggccaattc gcccttgatg aggaccgtcc gctttcgatc ggcaaggtgc ggagtttcta
    15061 cggcaacatc ggcgttctcc tcaggacgta tgcctacatt cgcacacaag gagcggaagg
    15121 gcttcaccgg gtcagtcagc gggctgttct caacgccaat tatttgctga gccaacttcg
    15181 caatatcatc ccggtgccac atgggtcccg ttgcctgcat gagtttgtgg cctcggcgct
    15241 gaagataaag actagccgcg gtgtctcggc catggacatt gctaagcggc tgttggatta
    15301 tggtttccac gccccgacgg tttatttccc cctgatcgtc aaggaggccc tgatgattga
    15361 acccaccgag acggaaagca aggccacgct ggatgccttg gtcgaagccc tgcgattaat
    15421 catgcaggaa ccgccggagc tggttcgcga ggccccgcat accacggcca tttccaggcc
    15481 cgacgaggtc caagccgccc gcaagcccat cctcaagtgg acaccctccg cagacggatc
    15541 gaatgggtga tgtgctcggc acccgccaag tgcttttcga aaaatgttat aaattctttt
    15601 ctttgtgcct tttcctatct tctacgataa gtggcaggat tttgccaaca gtttgacaga
    15661 cgcgctgggc atctcccgcg cggcggtgtg cgcgggggat ttgcgccaat ccacgcggtg
    15721 cccacggtac cccccgcttc ttagaaagac gcaaaaagag cacgtttaag acgaccgatc
    15781 gcgcaaacta ttgcaactcc gatgaagcac aacttaattc ctgcggcaca aaaatatcaa
    15841 aatacgttag aaaccaagcc tacgccaaat tgttgacacg tcgcacgaat gaacttatac
    15901 tactgctgtc aattgagcgg ttggtggttg actatgcagg gctctcgtct tgggttagga
    15961 ggtgtcaaat gaggtcaata ttaggccgtc tcactcgcgg gttcacgttg gtcgaactcc
    16021 ttgtcgtcat cgccatcatt ggcatcctga tcgccctact tcttcctgcg gtgcaagcag
    16081 cgagagaggc agcgcggagg agtcaatgca caaacaatct caaacagatc atgctagctt
    16141 ttcataacta tcatgacatt cataaaaggt tcgggcccgg gttggtgaaa atgggcaatt
    16201 ggttcgcggc taacaattgt ccagagggac aatgtgggac ttggtcttgg ggggctttca
    16261 tattacctta tcttgaacag acatcgcttt atcagcaact gaatcccacc atgattccgg
    16321 ctgatgcggc aatccaaccg gacgcggttt acaacattgt tcggcgccct cttcccgttt
    16381 ttcgtggtcc ttcaaacacg gccggaaacg aataccgccc gtaaggtacc gcgaattggt
    16441 ttctatggaa acgttgactg cacgaccggg tgtgctgacg tatttcttgc caactacgtc
    16501 ggctccaata acagttacga tctcaaccgc gagtacaccg gaaccgacca gttcaacggg
    16561 tttatgggct acggccagac gggtgaccgc ccacctcgtt gcacaccctt gggggacatt
    16621 tcggacggtt ccagcaacac attcgctatt ggagaacgag cctggcgaat tggcgtaaga
    16681 gagttgagag cagccgtgcc aatcatggcc aatggggaca ccggaaatca ctcgcttcag
    16741 gggcaagtct atgcaatggc atgtggccga tggccgctaa actgcacttt cgaccaagct
    16801 tgcgatcggg gattcagcag tatgcacgcg ggcggggcga actttgcgat ggtcgacgga
    16861 tcagtgcggt ttatctccga gacaatcgac cataaccccg ccacaaacgc agtagatagc
    16921 acgtacgagc gattgatcgc cattgccgat ggccaggcag tgcaattgcc ctgatttgct
    16981 ggcatccaaa gcttctacgg tatcgattgc agtccacgga gtttgctaag gaagagggaa
    17041 gaagatgcta gcaaggcgtg ctttatggtt cggggtaaca ttgacgtgcg tcctcgtggc
    17101 gggttgtggg tcgcgaggac cagcgttggg gcgggttgaa ggcacggtaa ccctcgatgg
    17161 cgttcctttg ggtggggcga ggattatctt cacgcctgtg gatggaggac gcgcatctat
    17221 ggcggttacg gatgggagtg gacattacga acttgagttt gcgggaggcg tgaaaggtgc
    17281 agtggtgggt aagcataagg tcagtatttc gacgtttgaa gccggtgaga aggatgacac
    17341 tggcaagctc gttggtcacg taccggagcg ggttccgccc cgttacaaca aagataccat
    17401 cttggaacag gaggtgaaac gcggacatca ggtgattgac ttcaagctag agtctcgatg
    17461 agttatccca tgaagcgatg ggtaaagcgt gagacgtaat agtcgtgaca atctagtatt
    17521 gggaggtgcg ttatgatgtt ccgaaaggcg tttactctgg tcgagttgct agttgtgatt
    17581 gccattattg ggattctgat tgctctattg ctgccggctg tccaggctgc acgggaggcc
    17641 gcaaggcgat cacagtgcac gaacaacctc aaacaattga tgttagcagt gcacacgtat
    17701 gtcgatacct atcagcgttt gcccgcccga caatcaggga gaggacatat ccaagcatta
    17761 gagcaaaggt tgaggatcag cggctgggtt caattggcgc cattttatga acaaaagtct
    17821 gtttatgaca tggccatgca agcgaacaat gatccgtgga ccaatggggc gtggcaaacc
    17881 cagatcatcc ctgtgttgaa ttgtcccagc gacagtggcg acatacctcc acgtggcggc
    17941 aagcggggat caaccagcta tgcattctgc tcgggtgata attatgaaac ttcggtacac
    18001 tcgcccacgg aacgttcgga tgcagcccaa tcagtcgccc tcatgaacac tcccataaag
    18061 aatcgaggga tgtttgggcg tcaggattat aactcgcttg ccaatgtagc cgacggaaca
    18121 agcaatacca ttgctctggc agagcgatcc cgacccaggc aggataatga taaaggttcg
    18181 gtgattgatg atcaaagcgg tgatccggcc agttatattc ccctggcctg ccgcgcctgg
    18241 tggtcggggc agcgatacat aagcggtgca ccgattttta cgggtgacac atctccgggt
    18301 taccgttggg gcgatgggtt ggccttcttt gcggctatta gcacgatttt gccgccaaac
    18361 tctgccgtgt gcatgttgcg gggtggtggt tggacggctg tgtctggaca ctttgccccc
    18421 ggcatatgga ccgccacgag tgaacaccca ggaggcgtga acgtcgccat ggcagatgga
    18481 agtgtgcggt ttatcagcga cacaattgat tgtggcaatc ttggtgtcgt tgccccaagc
    18541 ccaacggctg gcgggattag cccgtttgga gtctggggag cgctgggaac aaaggccggt
    18601 agcgaaacga tcaccggctt ctaagccttg gtgatcaaac gggtccagat ccctttggag
    18661 gatcggtcat tcaaaagctg tcggtatgct ttggggagac aaaacaccat gagtcaaaag
    18721 gtctggccag cggcaattgt tgtcagtgtc gtggtcgtgc tgtcgataac ggtaggatgt
    18781 caaaagcgat cccgcggcaa tattcccaaa accgtcccgg tttcaggcat tgtactttta
    18841 gatgggcagc ccgttgatgg tgctatggtt gtttttgtcc caacgcaatc accgggctat
    18901 ggtgcctacg caatgactga tagttcgggt cggtttcagt tgaaaagctc ggatgacgtg
    18961 atgggggcgg tcccaggaga ttatctcgtt caagtcacga aactggttgt ggacacggga
    19021 aagccccagt tcgtagtcca agaagacgtg gagcacgcgc aggcggcggg aggtggcgga
    19081 gccagtccag aaccaggggc aagcaaaaac gtgttgcccg aaaagtacag taatcccaag
    19141 acatcgggaa ttcagatcac tgtccccgca acggggctcg ataatgtcga aatcaagctg
    19201 agcaaaggag gctgaggtcc ccgcatcatc aggtccggta aaggcttggc atagtcgttt
    19261 gtcgctttct tcttgcggca aaggatgcag gaagcggttg agctgctcgg agtattcagt
    19321 gtgcgcgcgc tcgcaaagac ggcagggtca acgcttcgat cgccaagtat tgggcagact
    19381 ctcggcaatg tagctaaggt ttggcatcca ttcgcttttt ggttggccat ttatttagtg
    19441 ttgagccttc tcgcctatgg gcctgcgctc aggtatgcga cggcaagtct gccctgcggt
    19501 aaaccgaccc tcggtgtggt ggccctgttt aatgcctggt cgatttggtg gaatgccgac
    19561 cggcttaagg cgggcttggt cggttactgg gacgccccga ttttttggcc ggaacatggg
    19621 acattcgctt tttccgagcc ccagccactc agcttaatcg tggcacccgt ggtttgggcg
    19681 gcaggcccca tgatggccta ccatgcgtac tttctccttt ctttgtggct gaacggttta
    19741 ttggccgctc ggctgctgcg gcgactggga tgtggttggt ggggagccgg tctaggcggg
    19801 gcgttggtag tgtggttgcc cctggttcat cgacagcccg aaatgcttca atttgttccc
    19861 ttatggccgg tacttctcat gtggacaggg gctatccggc tcctgagtag tgaggcgagg
    19921 tggcatacaa tcgtggaatt cgcggcgggg atcgtgctca cgttcatggc ctgtgtccat
    19981 tatggggtgt tctcactgct tcttctggtt ccggcattct tggtggtcct gttacctacc
    20041 acagatcatg ggctgaagac cgcactgcag cgatccgtcc gggtggtttg gcgatgggtc
    20101 atcgggctaa tgctggcggg cgtgatagtg agtgcggtgt attggccgat gtaccgcatc
    20161 cttcgccaac acgagtttac ccgctcccca aaggtggttc aggccctttc tgcccggccg
    20221 gaacagtggt tggcggtacc tccggaggcc tacggtgggg tcgttgacct atcattcggc
    20281 gtggatcgtc ccttgcaccc tgggtggctg cggctggtac tggcggtggt gggcgcggtg
    20341 gtgattttga catatcacag aaagcagagg atcgcgcggc gcgggatcat gttcttggtc
    20401 gtgacggcgt tcttggccgt ggttgcctct ttggggcgaa acctggccct cggtgagttc
    20461 cgaccgtggg acgcactggc cattttcgtg ccgggaattg ctcaagtacg aagcccctat
    20521 cgatttgcct atttagcaca gcttgccgtc ttgctcctga caggggccgg tttcgatgtg
    20581 gcggccgtca agttactgaa tgcccgcaca caagggttgc gaccgatcat cttggccggc
    20641 aggccaagag tgatcgccgt aacactgctg agcggattga tgatcggaga ggttccccct
    20701 ccgcggatga tcttgactat ggcgcccgat ctgtctcatc ccccgggttg ggtgacgttt
    20761 ttaacccaga accgggaaac aaacgaaccg gttcttattc tggactttcc ttcgggcggc
    20821 caactcgtcg agtaccatcg gacagtctct tcgatgttgt ggcagaccca gtttcggttg
    20881 ccgctcgtca acggctacgc tgggtttttt ccccgaagat ggtcaaatct tgcaagcgtg
    20941 tggagggttt caccctatag ccacgaggcc ttccggttac tccaaagtct ggacgtgagg
    21001 attctggtac ggccttcgga tttcccacca ccgcccgagg cagatttggc ccccttccat
    21061 ttggaactgc tcatgaggga tgcaacaggt gtcgaagtct atcgactcgc atgggaagat
    21121 caatcagtgc cgattcgctg acgtacacat accgcaaagc cgtcggagta ccattggcaa
    21181 cctgagagaa ttgccgtacc atggcaattc atcgattttg cccggattgg gcaagttgga
    21241 gcaaatctct cctgtaaggt ctattccgcg cagagatggg tgtgggcgtg acgcatgtcc
    21301 agcgcttaag cgggcacata cctcgccaca accccttaaa tctcgaggcc aaatattcgc
    21361 ggtcgtatat cgaaacgatc acttttccgc gtcatgcggg acaaggggat gcgagggacg
    21421 aatatttggg gtcatcgaac gcgaagcggg gccagcgact gctgtgagag ctcgcaccgc
    21481 cgtcgttggg gcaaccatct ggcgaatcga cgcgccagct gaatgggcgt gcgcgatcca
    21541 tcgtcctggt gtcacaccga ttacgacggt taggatcaag cagatcgccg tcgcgaacaa
    21601 cccggctgcc gaagcgggtc ggatttccgc ttggggcttt tcttcctccg gggcaaagaa
    21661 tagcacggcg acaacacgca ggtaataggc cgccgcaatg gccgcattga tggcccccac
    21721 tatggcgagg atgatcagcc gagtcttagc cggattgttg ccaagagaac cgccaacatc
    21781 gagggcacta aagaagacag ccaatttccc ccaaaaaccg gccaagggcg gtacacccgc
    21841 caaactgaac atgaaggcgg caatcgccca ggcgatcaac cggagatcga atgctggcgc
    21901 ccgggccagg ccgcgaaggt cctcaagggt ttcaacgggc cgtccctgaa caccaagaag
    21961 gccgagagcg gcaaaggccc ccactgtggc cannnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnacgtcggg agcataaaaa tgaaagggca cggcggcaat cttgaacgcc
    22141 agtcccgcca aaataaacac cagggcaata ctggccagcg gtccggaaaa tccttgctcg
    22201 gccccggcaa tcatcagggc cttgtaacta acggatctcc ctagtccata gaggtaactg
    22261 acaccgaaca agaaaatcgc cgaggccaaa atactcaaat agaaatactt ggtggcacct
    22321 tcacgccggt gcggtccttc acgatccagg taaagcaaca cataggttgc cagtgagagc
    22381 aattcgagac ccacaaaaag cacaatgagg tcttgagcgc gaacagcgaa catggccccc
    22441 aggacggcca agatgagcgt gccgactcgc tccccatcat agctcacggg ggcaccacca
    22501 cttgaaagga gcaagaggaa ccctatgaac agaatgagtt cgcgggcaaa agcctggaac
    22561 agatcgggcg ttcgcgccaa atagtctgtg tgaaggacga cggccatcaa ggccgccaga
    22621 atgcccagtc cagctaggac ataaatccaa cctcggcgca gtccaaaagg ggacaggacc
    22681 agacaaagga caccagctaa gccgagaaaa acctcgggaa gccagtcagt cgtgattaac
    22741 agatgaaaca ccgcatgcat ctttattttc cgttaaagcc agtcgagctg tggcacgatc
    22801 ctgctcggcg aagcgcagct tcgcgtgagg tgacgaaggt tgcggcatca cgatgtgccc
    22861 caggacctcc tggccgtccc ggtcactcgg tgagacggcg agagtgatgg accgctcgat
    22921 ctgccgcacg gccggcgtca ttcgccgtaa aaagtaattg ggctgaatgc caatccacac
    22981 aatgaagacg aacaaggggg ccaaggccat aatctcacga aggtttagat cctcgattgc
    23041 gtgctgcccc gttgatacgt gataggtttt ccaatcttca tcgccaacgt gaggggcgaa
    23101 ggcctggcca tcccgctccg ccacaggcga tgccaaaggt tcgttgagag ggccaaagaa
    23161 cagccgacca acaagagtca gcatatacca cgctcctagg acaactcccg acgtggccaa
    23221 tatggcaagg gccaactctc caccgccgtg ggaccacgcc ctttggaaca tggcgagaag
    23281 cacgaggaat tctcctacga aaccgttgag gcctggtagc ccaatactgg aaaatgtcaa
    23341 aacgaatgtc atgaaagcga gaatgggtag ctttcgcgcc aggccaccaa aggcctcaat
    23401 cgtccgcgtg tgataccgct cgtagagcat cccgacaacg gcaaacaggc caccggtagc
    23461 cagtccatgg ttgatcatct ggagaagacc accgtggagc cccaccggac taaggcaaaa
    23521 gacccccagc atacagaatc caaggtgact gacgctggaa taggcgatca agcgtttgac
    23581 atccctctgg gcgaacgccg taaatgcccc gtaaacgatg ccaaccacgc agagtgtcaa
    23641 taaccagggc gcgaggacac gaacggcatc cggcacaatg ggcaaaacca gtcgcagaaa
    23701 gccgtatcct cccaccttca agagaatccc ggcgagtagc acactcccgg ccgtgggggc
    23761 ttccacgtgc gccagcggaa gccaggtatg gagcggcaca agcggcactt tcacggcaaa
    23821 cccgagaaac agtgcccaga aaatccagag ttgaaccgaa agcggaaacc gtgcttgcga
    23881 tcgcaactcg tagaggcgag tcgtaagttc aggaatggag aacgtcatcc ttcccgttcc
    23941 gctgtaagag gcggtccaaa tcacaacggc caggagcccc agaaacgtaa gcacacttcc
    24001 tgccagggta aaaaggaaga acttgatcgc cgcgtgacgt cgctcctcgt gtccccaaat
    24061 tccaatcaga aagaatagcg ggattaaggt cgcttcaaag aacacgtaga acaataggag
    24121 atcacgggcg gtaaaaaccc ccagcatacc ggtgtacaac accagcaaaa ggcgatagaa
    24181 ggtcggggcc tggcggtcaa tggcattcca actgaccagc accgatgtca ccatgagcag
    24241 cgaagtgagg gcgtacagcc aaacgctcag gccgtctaag cccagggcaa agcgaatacc
    24301 gatggtctgg tcggacctcg accaccaggg gacctcaaag accgcaaatt gctgtgcccc
    24361 cacaggaaac cgaaaggcga tccatccgct tagggcggtc gtcagaagga cgacgatcag
    24421 cgtcacacgg cgtgcgacct tttggctatc gccaacgatg aggacgcccg ccagcggtgc
    24481 gaagacgcta atggctaaca gtgttcccat tgccaaacct ttcactcctc cgcacaagcg
    24541 gagcttctaa cgaactgacc ccaaggcccg caaaatttcc cagcgttgaa caccacgatc
    24601 accacgttcc cattaaaaga acatcgcagc gagcaagacc aaaaccccga ggacgatccc
    24661 cagcccgtac cagggaatcc aaccattgtg cgaacttcgc aggagagccc ccaccagctt
    24721 gggcaggtgg ccaatgatat ccaccagcaa atcaatgaag tatcggtcga tccatgctgc
    24781 cactgccgcg agccacgtga ggggtcggac gatgatccat gtataaagtt catcaaagaa
    24841 gaacttatgg aaagatagcg cataaaggcc aatcgccttt gtcaaggcga tcacaggtcg
    24901 ggctacgccg cccggggcga ggacaaccag cgcggcccat ccgatgccac cgagggttag
    24961 ggccgtgctc agccacggaa tcaaagcggc accaccaccg tggtgcgccc caacctcgtg
    25021 tgcggcaaat gcggcaaggc tcggtgtcgc cgcgagaaag tgctcgatca ccttaccagg
    25081 accgaacgcg accccaacaa ccgccgaggc aattgccaaa atgacgaggg ggatcaccat
    25141 gacagggggc gactcgtgga ggtggccggc ggcctctgca gggacccgct cttcaccaaa
    25201 aaacgtgcga aaatatgcac ggaaggtgta gatggccgtc aacatagcag taacggctgc
    25261 cgccacgaac aggaagaagt aaagcgatga ggcacttccc ttttcataaa cgagatggag
    25321 gatttcatcc ttactgaaga atccagcgaa cgggcacaat cctgccaagg ccaaactgcc
    25381 aacaaggaac gtggcgtggg tccagggcaa tcgacgccgc aatccgccaa tttcattgat
    25441 gtcgatcacg ccgcctgtgg catgcatgac actgcccgcc cctaagaaca agagggcctt
    25501 gaagaaggca tgggtaaata agtgaaacat cgcggcagcg acacctacca acccaccgac
    25561 ggacagcccc aagaacatgt aggcaagctg gctgatcgtg gaataggcta gaatgcgttt
    25621 gaggtctgtc tgggtcagcg caatgatggc cgccatggtg gccgtgatga tggcaatgat
    25681 ccccacaatt ggcagggcat agggtgcagc ctcgaacagt ggcatacatc gtgcgaccat
    25741 ataaacaccg gccgtcacca tggtggcggc gtgaatcagg gcactcacgg gcgttgggcc
    25801 ttccatggca tcaggaagcc acacatgtaa aggaaattga gcgctttttc cgcaggcccc
    25861 tgcgaagagg agcaaacaaa tggccgtggc cacagcgccg tggacaagcc cctgctcggg
    25921 atgaacgagc cgggccattc ccagcactcc cgccgaatag acttcgccgg tttcgggatc
    25981 aattgtgtcg tgaaagttca gtgttccata caccgaccag atcaaaaaga tgccgagagc
    26041 aaatccaaaa tcaccgatcc gattgacaag aaacgctttc ttccccgccg cggccgcagc
    26101 gggcttgcga taccagaaac cgatcaagag gtagctgcac aggcccacgg cctcccaaaa
    26161 gatgtaaagg agcaagaaat tactgaccga gacgagcatg atcatggaga agatgaaaag
    26221 agaaacatag gtgaagaatc gccagtagcc gggatcatca tgcatgtatc caatcgagta
    26281 aatgactacc agcaaggaga cggccgtaac cgtcaccaac atgaagactg tcaagggatc
    26341 gacgcgaaga acaacgggaa tctgaaatcg cacaaagtcc accgtgccat gggctacggg
    26401 aacttgggga gacccgccgg tcggactgat gtcggcccag ctccagagtt ggaccacgcg
    26461 ctcaagaaca cgctgttcgc cgtcagccga atgctgggca agagtctggc ctaccaaaag
    26521 aagcaatgcc aatgacgcta tgaaggaaat ggctgttgcc gttacggccg gcacatgact
    26581 aaattctcgc agaaggcgcg ggccgagcac ggctgtcagg atcgcagccc caaggggcat
    26641 cgcggctatg agaataatga gaaatgtcat gggggttaca cactcggtcg atattcaaga
    26701 acttcctggg gaatatcggg acgccgtccc acccggggga gcctcggcca tgaaggtgtc
    26761 tcggcttccc tgacctcacc ttcatcgggc accggcggaa ggatgatatc ctcgcggacc
    26821 ttttgcaagc gtaggacatc caacccctct cttgccctca cgagcgtcaa aatcagtgcc
    26881 aaagcgacgg ccgcctccgc cgcggtgacg cccacaataa agagaacaaa tacctggccc
    26941 gtcgcttgac catgataacg gtcccaaccg gcaaaattga cgataacacc ttgaagcatt
    27001 aactcaacgg aaaggaacat gacgattaga ttccgccggg ccagaaaacc gacgaccccc
    27061 aagccgaaaa ggcacgtccc caccagcaag taattcatga gtaagccagt gttttccatg
    27121 gtattcccgt atggcttttg ctcctcggct taggattcat tcgcggtcgg cgtttcgtcc
    27181 ggcacagcgc gactcttgaa gtccagaagc ggttccaatg gatgggcgat gatcacggca
    27241 gcgcccagca aaccaagtaa gagcacaatc gccaaagcct gcacggccac accgtagtgc
    27301 aaaaacaatt ggttgcccag atcgacggtt cgcgtgatca caacatccgg gatcgcctta
    27361 cccgtctctt ttgccaaaga ccacccgtta gccgtggcgg catggcgcag ccaggtgacc
    27421 gtcgtcaggg caatgaggac cgcacccact acggaggccg tgagcggttc ccaactttgt
    27481 cgattgaaac cggctgtccc tttgggatcg tcaagcatga ggacgaacaa gaacatgacc
    27541 aaaatagcgc cgatgtaaac aacaatcacg gccatggcca caaattgcgc accattgaat
    27601 aacagcatcc cggccgtgcc caaaaccgcc atgccaaacc aaagggcggc atacaacgga
    27661 cgactgctcg tgatcatggc caccgccgag ccgacggcca caatagacat cagtgtgaga
    27721 agagagcgtg ctaacaggct gcccacggac ggaatcatag ccgaaagcat cgccagcccg
    27781 gcagccacaa gcacccagcc cacagaacgt ctccgtgcct ttccccacgg cagcgcccac
    27841 cacaggcctc ccgccgtgaa aatgctcgcc aagatcaaaa cgaagagtgg tgcgtccata
    27901 acattatcca caaacaccga agaaacgatc actcacttgt cctgtgtgag gtcctgatcg
    27961 tcttgcggaa gctctatcaa atcctcgatt tcccgttttc cccgcaacca gccgggaggc
    28021 tcttgatcct tggtctgatc atagatgctc aataacttct ccttgtcaaa gatcatttcc
    28081 tcacggctct tgccagaaag atcgtaaagg cttgtcagct caatggcatc tacgggacag
    28141 gcctcctcgc acataccgca aaagatacac cggagcatgt cgatgttgaa gacgaccgga
    28201 tatttctccc gatctggcca aggactagga ccagcgacaa tatcgataca gtgagcgggg
    28261 caagccgtgg cgcaaagaaa acaggcaaca catttcacgc ggccctgctc atcccggttc
    28321 aaacggtgaa caccacgata aatcaacgga tcagggaatt gatgccgttc ctccggaaat
    28381 tgaaccgtca acttcggccc aatgaggtgc cggaatgttg tcgtcagtcc atcccaaaat
    28441 aggggcaaat atgatctttc ccccatattg agtggtgact catcaaccca gcggatttcc
    28501 gagctgtctg gtttcatagg tcgttgatct catccaaagc aaagatctct ctttcgataa
    28561 agccttcttt gctttttacg catcgcacgg ccggaccgtt ggggattcca aatagggtga
    28621 accctcccgc atatcttggg caagacgcac caagtcgcgc cgcgggctgt tatcgtaatg
    28681 aggctgggca accgcagccg tgatcaccca agttaagata aaagtcaccc aattgattag
    28741 cccgaggacc ggcaacgacg accagccctg cgacacaacc aagggctccc accacgcgta
    28801 ccataccacc aagatcgtca cctgaacgat tccccaggaa atcaagggct tccatgcaag
    28861 ggtcatcaat tgatcaaaac gaaatctggg ccagctccag cgcatccaca tgaaaaagaa
    28921 caccaccgcc aggaccttgg ccacaaacac aacgattctt atgatcgctc caccgattcc
    28981 tgctaccggt tcggacccgg tgaggaacca aaggtgccaa cctccgagga agagcaggac
    29041 aatcaccacg gccgccgtga tcatgtggag gaattcagag accagaaacg tcataagccg
    29101 catgccgctg tattcggtat ggaatccgcc cacaagctct tgctctgttt ctggcaagtc
    29161 gaaaggcaac cgccctgcct cggcggtggc agccacgaaa aagaccacaa atgccaacgg
    29221 gtgaagaacg atgttccata ctccggactc agcttgttgg aggacgattt catcaattcg
    29281 aagtgtcccc gcataacatg ccaacgcgat cagagccaga ctcaaaggaa tctcgtaagc
    29341 gatcaattgc gctgcccccc gcatcgcgcc aaagaggctg tacttgttat tgctgctcca
    29401 accggcgagg atgatcccgt aaaccgcagc tccgttggcg gcaaacagaa agatcaatgc
    29461 ggcatcaaac gcgggaacga ccgtcaaatc gacgggcgcc catccggaga tcccaaggtc
    29521 caccgcccca agcggcacaa cggcatagac ggcgacggct gccacgaatg tcacaaccgg
    29581 tgcgacattg taaagaatac gatcgacgtg acctggaaca aactcctcct tgaaaatgag
    29641 tttcaaaccg tccgcaatcg gctgtcctag cccaaaaatc cgaatattcg ttaatgggat
    29701 gcctactcgg ttgggaccaa gccggtcctg aacccaagcg gcgatccagc gctcgacaag
    29761 gaccagataa gcggcggccg tcatcaaggc agccagcaac acggcaatct tgatgcatgt
    29821 cacaacggca aagctgatca tcttcagtcc tgtttgctat cagggcttcc taggtttgcc
    29881 atgtcacttt ttcgctacac gtccaaattg gtaacgattc tcactattct tactgcccag
    29941 tcgataccgt ttgctcggcc aattgattca ctcgaaggtc cacgccgcat tccgggattt
    30001 cgcccgatgc tgctgagaaa taagggatgg tccgtgcgat ttcggccaag acttcacgcg
    30061 gttgataaag cccgctcatc ccgcccaatt cccaaaaaac tgagcccaga cagcggctgc
    30121 caaatggcgg tcgaatcgcc cgcgccgacc actgaagccg gtgttgtcgg ttcacatagg
    30181 aaccttctcg ctcggcaaaa aacgccatac caaagatcag atcggcgcgg tcgctgaggg
    30241 gcgtgggaaa tacgtcggcg acaatcaacg ctgttgcccg ccgcagcgcg tccgcctgtt
    30301 ctgcgtcaat ccagggcttg atatagccgc caaggacgac aatcacatcg aaatgaccct
    30361 ctgctgtcct tgcgaggagg tcattaaatc tcaggggcgg ctgctcgaac cagtacgcca
    30421 cgccttcaac gcccaagcga ttgggacatt tttcgccaga aatgacaaag cccccgggaa
    30481 accgttcgtc ctctccctgc cgaggaattg gccctaaggc tgtggtactc tggggatgga
    30541 gttgcttgat ccaacggcag gccaaatagg cctcttcatt cgtcaagaaa ggtgaaaggc
    30601 aaagggctgg ctgccgcgct tgctgaacct tgtccaccat ttgaccgatg gcacccgccc
    30661 agtttattga cgagacgttc ccattttgtc gctgccaggc gccgcgcagc cgattcgggt
    30721 ggtggatcac agggtaactg tatcggccgt cgttacagat ccaccatcga ttcacgaagg
    30781 gattttcccg gggcttaatg cgatacaccc gatcctgatt ctcctccacc caaatggcac
    30841 aacccgtggc acataggaca cacacacttg ggtggcggcg aaggtaccat acacgttgtt
    30901 tatagagaaa gtccttgtcg gccaacgccc ctacggggca aagatcaacg acattgcccg
    30961 ataatttgtt attcaacggc aactccggga ggacgtcgat ttcttcatgc gcaccacgat
    31021 tcactactag taactccgac gtgcccgtga tctctcgggt aaagcggaca caccgggtac
    31081 acatgatgca ccgatcgaca aaaaggaaca catccccgat atcccgtttg cgactagtaa
    31141 acggccgaat ctcggcgcgg cgttccgact gaccaaactt gaagtgataa tcctgaagac
    31201 gacactcgcc cgctttgtcg caaataggac aatcgatggg atgccgaaga agaaggtctt
    31261 cttccaccat ggcgcgggcc cgcgcaactt tttcactgtt ggtgacgaag acggtcccgt
    31321 ccttcgcctg ggtattgcag gccggcatca gcttgggcag catgacaatc tgtccggtct
    31381 ttggatcgcg cgttcccacc tcgacaaggc acatccgaca actggcgacc acagataacc
    31441 ccggatgcca gcagtaatga gggatttcaa tgcccgcccg cgcggccact tgaatgccgt
    31501 tcagccgctc gtgcgcgccg atctccacct ctttgccgtc aacgatcaca atgcccatgg
    31561 aattcagtcc ctcatcttcc tcccaatcag gcaacgggtg ttcgcatgga atttgcttgg
    31621 tccgtctttt cgagagaaaa ggccggaacc gggcgcgtgg aacgccatcc cttggggttc
    31681 gtccgtttga tgtagtcctc caattcaccg cggaacttgc ggatggctgt cttgattggc
    31741 cacgccgctc catcggcaag accacaaatt gtggtgcctg gaataatgcc aatgttgtcg
    31801 gcaatctcca aaagtaggtc gaggtcaacc agccgaccgc gtcccgcctt gatgcgttcc
    31861 acaatccgta atgcccaagc tgtcccctcc cggcaaggcg tgcattgtcc acaactttcg
    31921 tgggcaaaaa agcgacaact gttatgcaag aagtcaatga tgagatacgt ttcgtccagg
    31981 acaatgacgc cgccggtgcc caagcccaga cagccggctt tgatggggcc agcaaaatcc
    32041 ataggcgtat caagctcatc cgccgacaga aggccggtgc tcagtccgcc cggcaccact
    32101 gccttggccc gccgtccacc cctcacaccc ccggcatatt cttcgatcag ccggcgacat
    32161 ggaatcccta aaggtgcttc aaaacaacct ggccgattga catggccact gacgcaaaac
    32221 aacttagggc cgaaacttcc tgggtcccgc ggatcattcg ggtcaggcgg cacaccgatc
    32281 gagcgaaacc actctgcgcc tctctccatg atgtgccgaa cacaggccag ggtctccaca
    32341 ttatttacca cggtgggttt tcggaataat ccttccaccg ccgggaaggg aggctttgtc
    32401 cgcggccaag cccgtttgcc ctcgatgctt tcgatcaaac cggtctcttc gccgcaaata
    32461 taggcaccgg cgccgcggtg gatatacatg tccaaatcca agcccgtccc caagatgttc
    32521 ttccccagat aacccgcctg ataggcttcc cgaatcgcct cgcttaaacg ccggtaagct
    32581 agcggatatt catatctcaa atacacgtac gctgttgtcg cccgggttgc gtacgcgcta
    32641 atgatcgtcc cttccaggac ctgatgggga tccaattcca aaagaatgcg attgcaaaat
    32701 gttcctggct cgctttcatc agcgttcacg caaaggtaaa ttggccccgg gtgatcggct
    32761 ggaagaaaag accacttcaa acctgtcggg aagcccgctc caccacgacc gcggagacca
    32821 ctaatccgga cttcctcaac cacctccgcg ggtgacaatt ctttgaggac acggcggagc
    32881 gtcgcgtagc caccgctcgc ctcgtaagct cgcaaggtgt ggctgtctgg cttttgaata
    32941 tttgccaaaa ggacgggttc aaacgattcc atcatccatg tccgtttatc tcttgcttag
    33001 aaagttcatc gaggactgat tccagtttct cccgcgtcat cgtgggatag agcgtttcat
    33061 taataaggac ggccggggcg tattcgcagg ccccaaggca ctccgccgct tccaaggtga
    33121 aacgcccgtc cgcagttgtt tcgccaggtt cgatattgag tcgttcgcgc aaataatcga
    33181 gcaggttttc cccgccacac aaggcacaac tcagagaacg acatacccaa atgcggtact
    33241 ttccgagcgg ccgatcctgt ttgaagaatt ggtagaatga cagggtgtct tcaatgtccg
    33301 aggctggaat tcccaaaacg ttggccaact ccagcacggc gcttttgggg acgtacccaa
    33361 gggtctcttg gatcaggtga agggctggca ggacaacggc acgcgtgttc ggataacggg
    33421 ggaaatactc gcgaattgac gcaatgacct ccgggggaag caaggcgccc cccctacctt
    33481 caaccgtttg tgcctttgat tcttgcgaca ttgattcacc ccgtatccag ggcaatcttg
    33541 ttcaccgatc caattccgcc gcgataatat tcaaactccc caggacagca acaacatctc
    33601 ccaacatgtg gccccgaatc agatgcggaa agagtgcaaa gtggacaaac gaaggcggcc
    33661 gacaccgtgc ccgataggca tagggcgaac cgtcaccgac gatgtaaaaa cccaattccc
    33721 cgttgggtga ttcggtcgca cagtaaactt cttcacacgg cacttcgaaa ccgcggtttt
    33781 gcatgatgat ctcgaaatgc gtgatcaatc cctcaatcgt ggagtgaact tgccacttgt
    33841 ccgggataat ggcccgctgg ccaacatcca cgtggactgg tccggagggg aggttttcca
    33901 cggcttgatg aatgattttc aagctttctc gcatctcggc catgcggacc aaataacggg
    33961 cgtaacaatc cccgccctgg gcgcaacaca cttggaagtc aaaatcagag tacgctaggt
    34021 aaggttcatc cttgcgcaaa tcgcgggtca ccccactggc acgcgcgatc ggccccgtaa
    34081 cgcctcggtt gatcgcctcc tccttggtca gaacgccgat ccctttggtt ctatccacaa
    34141 atacccgatt gcgcgtgagc agtcggtcca tatcatccaa tgcccgaggc atcttgcgaa
    34201 ggaactcgcg aatcaattcg atggccttgg gcgaggcatc gtacattaag ccacctacac
    34261 gggtatagct gtttgtgaac cgtgccccgc aaagggcctc gaaaatgtcg taaatcattt
    34321 cacggggatt aaatgcgtac atgaaaaagg taaacgcccc cgtgtccagc ccaacggctg
    34381 cattacagac gaggtgatcg gcaattctcg ccaactccgc cacaatgaca cgcaaatact
    34441 gacatcgcgg cgggacctcc agccccatga gcttttctac cgcaaggtgc caggccacgt
    34501 tattggccat gggcgatacg taattcatcc gatcggtgat tgtgacatac tgattgtaat
    34561 tcaggtgttc cgccagcttc tcaaatccgg aatgaaggta accgatgtca cagtcggcat
    34621 caacgacgcg ttcgccttcc aatttcagaa tgaggcggag cgtggtatgc gtggccggat
    34681 gctgtggacc gaaattcatc gtccacaggt attcctggac ctcgtctgtc gcctgtgaga
    34741 gttcgcttcg ttgaagcggc atgttttcca gctttcctat ttcctcaagt cttcttaatg
    34801 tgaacctctc ccgaaggaga cacacgcccc ttcatcccag atcccgcgtg acaacaggga
    34861 tgttgtgccg ttcaccccgt ccttgaagcg gataatcctt tcggagaggg tgatcttgaa
    34921 agtcttcggg cagcaagata cgccgtaaat tggcgtgacc ttcaaaccga ataccaaaca
    34981 tatcccaaac ttccctctcc agccaacggg cgccttccca aagatcggtc actgtgggca
    35041 gagttggttc cggatcatta acgaatgtct tcacgatcaa tcgatcggcg ttttcaggac
    35101 ggcccacaat gtaaaccacg ccgaaccgat ctttggcctt cgggtaatac agataatcaa
    35161 cacacgtaat gtcaatgatc agggtaaagc cgcactcgtc cttcaaaaag cggagcacat
    35221 catgaacgat ttcacggggc acgcaaaccc gaaattgctt gcgaaactcc gtgatctcgg
    35281 ccgatggaaa cctggcctgg atcgcctgga ttgtctcgga tgacaccatg tttcagtcac
    35341 ttccgtagat aacaccgatt ccaaacaatc ctcttgtaca cccaattcga ccgacggcta
    35401 agacgtcatg gcggtgattg ccgcgattcc tacgagtcgg gagactcccc acgtttgccg
    35461 gtatcccact tattcgctgc ttcatcttga gcaaaaatcg gtggaccggc aacaagcaaa
    35521 cgagggtttt tgggatcggc caaaagtgcc cgcggtggct gatgtggtct cctctccaca
    35581 tctttccccc aagccgaacc ttcgcgttgg atcttttcct gaaggtcaat aatcgcctga
    35641 aggagctgct ccggacgcgg tgggcaacca ggcacgtaca tatccacggg aatgaatcgg
    35701 tcaatccctt gaaccacggc ataggtgtcg aagacgccgc cggtcgatgc acaggccccc
    35761 atggaaatac accacttcgg ctcatgcatc tgcagccaga tcctttgcag gaccggcatc
    35821 atcttcatca caacccgacc cgccacgatc ataagatcgc actgcctggg gctaaaacgg
    35881 aagacctcgg ccccaaagcg ggcaatatcg tgtcggctgg cacccgtggc cattagttcg
    35941 attccgcaac acgccgtcgc aaacggcatg ggccaaagac tgtttttgcg acaccaattc
    36001 accaaggcat cgagcttggt aatgacgaca ttgtcgggca agtcagtcat tcttgtcatt
    36061 gaggtcatcc accattagga tgttgccccc catcacttcc aatcacttcc aatcaaatac
    36121 ccctcgtcgc cagtcgtagg catatcccag accaagcagg acgaagaaca ccaatcccgg
    36181 ccagaggatg ccttggagcg agcgttggag cagtgccccg gtcgccaact cgccagattg
    36241 ctcagacgga ctttcgtggc tgcccaagcc caccacaact cccgcgtcgg accgcccggt
    36301 tgtttccgtg caggaactca aggccgcagc cttactttgc caggaactac tttgcctcag
    36361 cgatacgacc catggataaa gaaaaagtaa ttccacatca aagaccagaa acgctatagc
    36421 gaccagatga aaccgcacat gaaagcgacg ctgggcatca tggaaaggat ccacgccact
    36481 ttcgtaaggc atggccttga caggattcgc ccgatgcggt ccacacaaat ggcctaagac
    36541 gatgagaccg atactcaaac ccaccagggc catcaagaac agcgtgaaag gaacgagtgc
    36601 cgcatcgaac tccatcgctt cctcgcttta agccaaggat ttacttttcc gcacattgtc
    36661 gcgaagaggc accatatgca ttgggccata tctggcccgc agcattggcg acatgtcgca
    36721 gcatccgtgg ctgatcccgc cataacagga ccttcaacgt ccgtgacgta ggagttcttt
    36781 tttctgtcta taggcaggta aaaccacgat caaccatttc tggcaatcgc agtcgtgtta
    36841 ccctttttac gtcacggggc aaagcccagt tcgtctccgt tgtctttcac cgccgtcacg
    36901 tgaaacgatc catcgtccag acgctcgaca tcgcccaaaa caaccgaccc cggcaaacca
    36961 tcgcctcggg aagacctctc ccacgatgtt gacaactgtg actattgtaa atgtcgaatt
    37021 cactcgtatg gcattctagg gtcacgatga gacgtcgtca actcggacct gcgcaaggtt
    37081 acccaagcgc catcgaactt gataaatcac ctccaggaca agccaaccga agcgtcactt
    37141 cttagatcgt tttacgataa tccagcgcat ttctcccgct tgatccatgc ggataggcac
    37201 gccgttccag gcttcctccg cagttgccaa gcaaacctcc ttctcatctg cgttaaggag
    37261 gcgaacctcc tcgcaatcat caatcgtgaa ccactccgga aactggttga tacgtggata
    37321 atcaatcggc aaatggaagt aatggcggtg tcgtgggcga tcaaaaacaa gacggccctc
    37381 ccaaggtttg gaaacggtta gggtaacata aagcctcccc tcatgaagca cagcccctaa
    37441 gcggacgtct tcgcgccagg gctcgacccg tagtccttgg gtcttccaaa aggcgtacat
    37501 aattcccgtc cgcgcaacat tgccgtcccc atgccaccct tcgatgatgc cactcggttg
    37561 ctgaggacgc cacatgtagt gaatcttccg gtcgatccac gcagccgccg aagaatccgg
    37621 ctcacgattg tagagattga tggcactttc gatgctatcg gcgtactcgt cggctgaacc
    37681 ccagtggtaa tcgcctactt gggattccaa ctgagaaagg acgaagcgaa cagcctcacg
    37741 gtatcccggc gtcttatcta ccaggtacac agaataaatc ccgttgtagt tgtagcccca
    37801 ggtgtcgcag atgttggaat cgtgttcacc tgtcagcgga ttgatgctgt tatataacat
    37861 tccgcgagca tcccgtccca cttcaaggat gcggtcgcac atggcatgga tgggctcgcg
    37921 gtattcctcg gccttggccc gatccgcaaa actgaccgcc acatacaact cgcacagacc
    37981 agagacaatc tcgcaaccgt ggtcgcggag tcgcagattc tgcatattcc tagtgggatg
    38041 atgagaaccc aagaggtaat aatcgcctag ccgcttggcc caatccagat actttctgtt
    38101 gcccgtcagc caaaataacc gggaaagaat ttggagttgt tcaccgttga cctccacgtt
    38161 ttccgaggga attgctcccc acggcgtctc aaccggggcc actttccata tatcatcaac
    38221 aatcgcgagc attcggtcca accatggcga cgggcccaag aactccgtaa gaggcaacaa
    38281 gccgtctttc acatactcgg agcccccgaa aatcaaagcc ccaagctgtg gctccggccg
    38341 aacgaaaccc tgcttcgaga acgaccatgc atcgggaatc gaaccaaggc ggctcgtcag
    38401 acgcgtttca gtctccagca ttgccaacat gcggccatca aaaagcccac gttctgtcag
    38461 ataactggtc aaaaccatga acgggtagtt gtcagccgcg gcatcctccg gattccaaat
    38521 atcgcgggat tgggtgaggt ttctggggat gagacccgtt ttaggatcag cctgctcaag
    38581 ccagccctgc acgtacttat tggcgcaaaa aaacatctcg ttggccagcc gcccatttct
    38641 cgctgcgcaa cgaaacgccc attcagcaga gccttcgggg acctcatcgg gagaaagtgc
    38701 ttgcggatca cgccaaatgg cctgcatttc cgggtaccga tcagcaaaac caccatccac
    38761 tggtccacca accgtgaccg gcaacaggtt cacacggaga gtgaattttg gaagtgaacc
    38821 ctccgtcttc cctccttggc agattaaagc agcatgcgag aagaaggccg ctttgtctga
    38881 ggcccccgaa agacgaaact ccaacgaatc cccggatctg gcgggaagtt caaaggccaa
    38941 gtcttgaaaa ccgtcacagc ccccgtaagt cgtccgtcct gactcgatgg tctttccttg
    39001 ctcaatccac tcataagcca ggccacgccg atcattcttg gcccctgctg agagttggag
    39061 caccaatccg tcaaccaaag gcccgttgat ctggatggaa acgatgtatt ttcccagcca
    39121 aacatagggt ttccctgcgt aaacttgggt tgcgtcagcc ggatcggaaa gggcgacctc
    39181 tgccagcact aaatgttgcg ccgaggttgc gccttcccca aacgattgtg cagcgcatgc
    39241 cgccgaatgg tagatcccca acaggacgag aaataaaacg aacaacctcg ccaaaccggc
    39301 gaacctggcg tgtgcccatc ggaatttaaa ccgcgcagca ggcgtgtcac gatacgacca
    39361 acgatcgtaa gccgcgtaaa gcagccaaaa cggcttgccc tgggaaccca tgccacacct
    39421 ccgtagggta tttgtgttaa ctgtccggaa gatttggcgt ctgttgtagc acatcgatcc
    39481 gggtcatgca tctggggtca aaacagttga tccgaatact gacggacgag gcttgcctca
    39541 aagcaatttg gaacttacat cgggtcacca aaagacctgg ctctatagtc actgctatta
    39601 tcaaaaagac gacacacccc ggtggggtac cctccacctc acgtttgtct tggaaaagcc
    39661 cgtaaattgt gattggcaat tctcggcact tccgatacac tttaacacgt taagcggcgc
    39721 gcatgcgccg ccggcttgtg aagatagtag aggacacacc caagcggggc ttgtgcagtt
    39781 tttcccccgc gcggctagcg tgtcaagtca agcgtcgagg actatttcgg caaaaagcta
    39841 ttctgagaca tggaggtcga tcatgggcgt tcgcattcac cccaccgccg tggtgaactc
    39901 ttttgccgca ctagggaccg atgtggagat cggtccattt tgtgtcgttg aagccgatac
    39961 catcatcggg gatggttgtc gtttagaaag ccgggccgtc gtcaaaagcg ggacgaccct
    40021 tggcgaaaac aactgggtgt ttgaaggggc cgtcctcggc ggaatgccgc aacacgtcca
    40081 catgcccgaa cgaccaggcc gagtcatcat cggctcagga aacgtcattc gagaaaacgt
    40141 aacgatccac cgagcattgg acgaagagga tgccaccctt atcggcgata acaacctttt
    40201 catggccaac gtccacatcg cccatgattg ccgcgtcggt aacaacacga tcttcgccaa
    40261 taacgccatg ctggccgggc atgtgacggt gggcgaccga gcgtacgtct ccggtgctgt
    40321 ggcggtccat cagttttgcc gcattggggc ctacgccatg gtaggcggcc aagcccacat
    40381 cgtcaaggat gtgccgcctt acgtaactgt cgatggatta agtagttacg tcgtcggctt
    40441 gaaccagatt ggactccgcc gggcggggtt ctcaacagag gccatcgccg agctaaaaca
    40501 ggcatatcgg gtgatctacc gcagcggcct gtcttggaag gacatcctcg aacagttgag
    40561 aaccaaattc agcaccggtc cggcaagcct cttttatcaa ttcctgtcaa cgaccacccg
    40621 cgggattatc caagaacgtc ggaccccgca aggcgtgacc ctcaaactcc gccgggctga
    40681 cgacgaagat tcggacaccg ccgagtggcg tgtgcggatc accggttgat tcatcgacgg
    40741 tatcgcgcag ggcattcgtc cgcaacatca agcctaaccc cgtatccggt cataagttat
    40801 ttcgtaactt gcagttcggt actattctct gcccacggat gctgtgccgg actatgcgcc
    40861 ccttgtgcgc cgctttcgct cacttggacc gccgtctcac gccgacaacc ccggatgcac
    40921 ccggcgcaat accgaagcgt gccccgcccg gttgcgccgg tggagaccga gcgaagacat
    40981 tctcaggaag ttctgtctcg tccgcccggc cgtgatctca ctcgacacgt tcttgcccat
    41041 caccgttttc tgacgggtcc agctccagca ggaagaggtg acggccggca ccccagacat
    41101 gaaccccggc atccaaaacc gggttcgccg gcgcctcgct ctcttgcgaa tccgccgggg
    41161 gtacgacagc agaaagtcct cgccgctgcc gggcacgcgc ggaattcgcg gcaaataccg
    41221 cggcaccagt tcttccagcc gaccggggta tcgaccgtgt tccaatttgt acatgcgaag
    41281 ggccaggcga agacaagtcg cctgaacatg gaggtcgccc aatgctcggc tgaaaaaggc
    41341 gtcttccagc gatcgccgaa tggacgcggc ggcgttccat ccgacccccc gccacagcga
    41401 gaccggtttc gacgactcca accgcctcct aaactgccac gggaagacgc cacgctcgtc
    41461 gtattctacc gcgcggggaa gatattccgc gagaatgcga tcgagccggg cttgatggag
    41521 ctgccattcg ccgggaaccc aagacacggc gatagggagg aaccacagct cgtcggacca
    41581 ccaggcgaac ctcggccctt cgcgccccat gccgatcggg gcgtcgcgca ggaacggacc
    41641 tcgccaatac gtaccgagca gcagcgtgcg gacttcgagc ggcacgcgct ccctcacgac
    41701 gacatcctgg atatcctgca acatctcgcg gagggattgc ggcgtttgcc cctccgagcg
    41761 gctccattcc cacgcggcgt cggcccagcg gctccattgg gccaggtctc ggccggagac
    41821 aaagaaattt ccaaacgcgg cgggataggt ccgttccgca cgaaatcgca gggccagcgc
    41881 ggcgatcagc aggctccgcg actcttggat ctttcccgcc cggaccgccg cccgggccag
    41941 gttctccaag gccggaatcg tgatcatttg caattctaat cccaatgtgc ccgcctgaga
    42001 attaacatcg agccgctccc acggcgagcc atgccgcagc gcgtccacga gcgggtcggc
    42061 atagggctgc ttggaaagtt cccccaagac ccggaagaaa tccaccgccg actcggattc
    42121 caaaaaccgc tgtcccggat cttcccccag ttcgtaagac cacgttcggc gcccaacagc
    42181 caagctggac agcggtccgg aaaatttgac gcggcgcgca acttccatcg tcgccgcgaa
    42241 accttgaagg ctctcgggct gtggctccat ccattgctgg agccttacct gcgccgcggc
    42301 ttccatgttc cggagcgact ccacgtccgt ccgcggaaga aacatcacgc gggtccacgg
    42361 caggatgagg aacgtcacga ccaagcccca taggatgggc gagctaagcc ggtcccggaa
    42421 cccgccggcg gctcgctctt ccagccaact gcggagggac cggcgggatt gccaaaagaa
    42481 cgcggcaagc aggatagcca cggtaatcgc gaaactccca ccggggatga tctcatccgg
    42541 tcccacagga aagaacatca cgggcatcgc aacggccagc gtcaaccccg cggcaaggat
    42601 cagggcatgg aggattccgg gggcgaacat gccggccaac tgaccagcgg cgtataacac
    42661 cgcaccgccc agcagagaat agaacaacgt cgcgccgagc gtgccgtcac ggttgaggtt
    42721 cgtatccgag gtgacggccg aaaggcaaat ccaagccgcc agcaggatac tccagaccaa
    42781 gcaccagcgg acaccccagc gctggcgcgc cagccagatg cggc
//
LOCUS       DAOSUT010000008        42689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_627_length_42689_cov_9.236220, whole genome shotgun sequence.
ACCESSION   DAOSUT010000008 DAOSUT010000000
VERSION     DAOSUT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 42689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42689
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_42689_cov_9.236220"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..1228
                     /locus_tag="PLD05_01430"
     CDS             410..1228
                     /locus_tag="PLD05_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOP76116.1"
                     /translation="MRRLLVKMPVVLTLVVVMVSLQAGGSFCFGQMYKDLHAKLEKKV
                     YKDSEGGTLPYRLWKPADYDPAKKYPLILFLHGAGERGEDNEAQLKNDEFLTLVLDPE
                     HPAILIAPQCPSKLWWSAIRRPWDQPESGNPEPTPPMKLVMGLLDEVRKELSVDPDRC
                     YVTGLSMGGFGTFDLLLRRPNDFAAAVPICGGGNVERAAEIAHIPMWIFHGGADPVVP
                     VKLSRDMVEALKKAGGEVRYTEYPGVGHNSWSKAYQEPELRKWLFEQVRKKKDG"
     gene            1329..2573
                     /locus_tag="PLD05_01435"
     CDS             1329..2573
                     /locus_tag="PLD05_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76117.1"
                     /translation="MAEQSRNVQGGSRTARHGANRRQFLAAGGAVAVSILEPSLIFGT
                     QANEKVRLGLIGCGNRGRWIARLFRDHGGYVVSAVHDYFPSRAKAVGGEFAITEDRCY
                     SGLLGYRRMLDAGGIDAVVIESPPYFHPEQAVAAVEAGVHVFLAKPVAVDVMGCQTIQ
                     TAGEKATAKKLVFLVDFQTRTSPLYQEAVRRVREGDIGTIVSGEAVYYCGPTWVVPED
                     FDPKNPEDLLRAWGAFRVLSGDVITEQNIHALDVATWFLDAAPLRAYGMGGRKGKTGP
                     GDCWDHFSVIFTFPNDVLVTFASKQYGAGYDDIGCRIFGTEGTADTHYFGTVSIHGKK
                     PFPGGSTGSLYADGAKANIKTFYDAIKNGDFSNPTVAPSVRSNLTTILGRMAAYQKSE
                     VTWDQMLASQERWEFDVTGLKE"
     gene            2570..4015
                     /locus_tag="PLD05_01440"
     CDS             2570..4015
                     /locus_tag="PLD05_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76118.1"
                     /translation="MIGDAVTDLVLHRTRAAWLTLGIPIVTALAGVAAIVYWLVAVPE
                     PKLSPRVPGLDRPLQRRTGTTVKPKLTGTLVRGDGSPSNIPGEWPRFRGPKLDAIWED
                     GTVTLARSWDADGPPRVWSIAVGEGFAAAAIRDGRVYLIDYDREKEQDVVRCLSLDDG
                     RDIWQFRYPNTVKRNHGMSRTIPTVTEKYVVSIGPKCHVTCLDRTTGESRWLIDMVDR
                     WGTTVPPWYTGQCPFVDQDCVILAPGGPEALIVAVDLETGEERWRTPNPRNWKMTHSS
                     IIPMESCGHRMYVYCASGGVVGVDAATGAILWETAEWKISLATVPSPLPLPGDRIFLC
                     GGYDAGALMLQLRKEGEKITPEVLYRLPPQEFGSTQQTPVFFAGCIFGVREKDKQMVC
                     LDLDGKVRWSSGSQYRFGLGPYMIANGLLLALDDDGTLTLAEASPEAFKPIARARVLD
                     GHDCWGPMAIVKGRLIIRSMTEMVCLDLTSP"
     gene            4154..5269
                     /locus_tag="PLD05_01445"
     CDS             4154..5269
                     /locus_tag="PLD05_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76119.1"
                     /translation="MVVVVVAAAAVGAVAVWQYRNHLRNPSRDLKAERFSYSLDLYQE
                     IPPERIGFEPAWQLAIPGDQIRRVTVFQNTAWVCADQSLIPVALTSEGGAVGSPMSVP
                     VTPYCLAVAEWPDQDNRAERYFVVGGGNQVLVIRETGAVVGEWSFFGRKANITDLAIA
                     DEDVFLADAGNRVVHRVDRFGEKVAEIGHRDAERGILGFIIPSPYFSLAWNGDGLLRV
                     VNPGVHRIEFYSPDGDLETFWGKAGLDEAGFCGCCNPARIALLMDGRIVTVEKGIPRI
                     KVYSPHGDFICWVVLPSYFTGSAQWKETRDQERLPVLDVGVFQVVEDRGEGEEWIVIL
                     DPARKVLEAFRPKRPEEPNDKTPSSGAASAGTPDEAH"
     gene            5642..6229
                     /locus_tag="PLD05_01450"
     CDS             5642..6229
                     /locus_tag="PLD05_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOP76120.1"
                     /translation="MSNAQSRQRIPRREILREGVQMAAFLGVGGLVGFVAGRKATSEP
                     LVWQIDPYKCIACGNCATHCVLDPSAVKCVNSFPICGYCDLCTGYFPPEPAALNTGAE
                     NQLCPTGAIIRKLVEEPYYEYTIDEELCIACGKCVIGCAAFGNGSLYLQVRHDRCLNC
                     HECSIAIACPSQAFVRVPASEPYLLKHKYKPAAPA"
     gene            6376..7725
                     /locus_tag="PLD05_01455"
     CDS             6376..7725
                     /locus_tag="PLD05_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOP76121.1"
                     /translation="MSRFSIGLLAIILALVWTYASRAVERFPPPDFTSHQLPETPQPP
                     PPAPWWETIDVVLLVTALVLATYFALAKRSRNATLVLTGFSLFWFGFHRQGCVCPIGA
                     IQDVAQGLFDSAHAIPWSILVFFSLPLVFALFFGRVFCAAVCPLGAIQEIAAIRPHKL
                     PDWLEQMLGLFPWVYLGLAVLYAATGTAYVICEYDPFVAFFRRSGATSMLVFGGALLA
                     IGIFVGRPYCRFLCPYGVILGLFAKLARWHVRIPPTECIRCRLCEDACPYNAIVAPVP
                     PLTRSERAPARRRLLLAMAVLPIWLALGGGIGYALHPIMAQLDPQVRLARWVRSKNID
                     PNNKFAVDAVTAFHNTGASEASLYQAAAERLRKFGVGSAIFGLWVGLVFGLKWVHTST
                     RPSRQEYLPDPRRCVACGRCFWYCPVEQVRRGWIIEDELLQLPKERAPLVSSVANDE"
     gene            7789..9690
                     /locus_tag="PLD05_01460"
     CDS             7789..9690
                     /locus_tag="PLD05_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76122.1"
                     /translation="MMSQRREESNVPDKGEAQLAPANLRVETPGALVAPLPGFLAALR
                     TAIVAAIFVFVVVVLLVWNALHQVNKDSLDSPQYAELQKKLSLDPQNEELKQQIRELD
                     LQLRQAYFRSRTFNRWGTMLLIAGLIVFAVAARIASGLRRPLPRPTATEIWDESFDRA
                     TRLRRFAILGMAGLLVAVACVFWAVGRSVIKDTLAVREAAEVPPREVAAVVTSPTEST
                     VVQNGSRGMGSSQQGKAIFTEEYRRYWPRFRGPDGSGISPHPNPPVSWNGKTGEGVLW
                     KVAVDLPGNNSPVVWANRVFLTGATEERRCVYCFDADSGKLLWQQDIPATPESENKKP
                     KVMKDTGFAAPTVATDGQRVYAIFANGDLAALDFEGKLLWTKGFGVPDNAYGHASSLC
                     TYGDLVIVQLDQGTPDDDLSILAALEGASGNVKWEVKRPTGNAWTSPIIVSSAGKDQL
                     ITVADPFVISYDPLTGKEWWRFEGTTGDCGPSPVVHRDLVIAGGEYSYYMFAIRADGT
                     GDVTETHKVWEGEEALPDACSPLAFDHYVLWMSSGAVLACYDIEKGEMLWEHEFPENT
                     FYASPALADQKVYLFSKEGPCFVGVVTDEEFKIEHTNELGEKCVTSPAFQPGRIYVRG
                     EEHLFCLGQ"
     gene            9853..>10656
                     /gene="nuoE"
                     /locus_tag="PLD05_01465"
     CDS             9853..>10656
                     /gene="nuoE"
                     /locus_tag="PLD05_01465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOP76123.1"
                     /translation="MTANAVDRGTATVDLNIVDEIVERCGRKPEAAIPILQAIQAHFR
                     YLPQEALERVCQLTEIRPSQLIGVATFYGQFRFRPVGRHIIHVCHGTACHVKGAERIQ
                     EALERHLGLRPGEDTDPEGLFTVEKVACLGCCTLAPVIQIDRSTYGRLTPAKIPRVLA
                     DFLHAQSNGSHPSRGPSAGDFEEGEVRVGLGSCCQAQGSAEVYRRFEELVTLAAVPIS
                     IKRVGCVGMCHQTPLVEVVPRRGKSQIYAKVEPDDVPAIVHRHFRPRKLW"
     assembly_gap    10657..10756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10757..12178
                     /locus_tag="PLD05_01470"
     CDS             <10757..12178
                     /locus_tag="PLD05_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOP76124.1"
                     /translation="KATGEPKYVTCNGDEGDPGAFMDRMILESFPYRVIEGMLIAAKA
                     VGAHEGIFYIRAEYPLAVERVRAAIHICEERGILGDRVLGTDFRFHLRVNEGAGAFVC
                     GEETALIASMEGRRGMPRLRPPYPAEFGFGGRPTLVNNVETFALVPWIIRHGAERFAR
                     LGTATSKGTKVFALAGKVRRGGLIEVPMGITLREIIEEIGGGALPGRRIKAVQVGGPS
                     GGCVSEALFDTPVDYEALTATGAIMGSGGLIVLDNTDCMVDIARYFLRFTQDQSCGKC
                     TLCRIGTRRMLEILERICNGEGRAGDLEVLEDLARTVSAGSLCGLGKTAPNPVLTTLR
                     YFREEYEAHLRGECPAGKCSPLIRYVIADQCIGCTLCAQNCPVGAIPLRPYRRHAVDD
                     AKCTRCDTCRLVCPIGAVVVESGGRVVAYGPMAQTNSTTSPGGPTSASGGTCATLPQF
                     VPLSEGRSGIVATATSQNSGRAL"
     gene            12175..13824
                     /locus_tag="PLD05_01475"
     CDS             12175..13824
                     /locus_tag="PLD05_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP76125.1"
                     /translation="MNEAHCRVTIDGRIIEVPRGTTVLQAARQLGLDIPTLCYLEGLK
                     PQTSCLVCTVKVIENGRARMMPACGTPVSDGMVVESETAEVAHVRRRALELILSDHVG
                     DCFAPCHYACPARMDIPTVLRHIQVGQLSEAIATIKRAVAIPATLSYVCPRPCEKGCR
                     RHYLDEPVMICQLSRFVAEKDLTSDAPYSPPCQPNSGRAVAVVGAGPTGLSAAFYLRQ
                     AGHQVTIFDSAPQPGGRLRQMIGEDAGRVPIDILDAEIALILRSGVEYRGETPLVTSE
                     QSGLTLTLLTRQFDAVLLAFGEQSQGFYRAIGLACDNRGLVVDRGTFQTSLPQVFAAG
                     SAIRGRVSVARSCADGKSVAECIDAFLREGHPRNVTEPFGVRMGRLEKEELDRLAQVA
                     ARSGKETPPQEWDIETAREQAGRCLHCDCRALPVCRLRQYAERYGVDPNRYRAQRARF
                     EIVSQPSGVIFEPGKCILCGICVELTESARAPLGLTFIGRGFDVRIGVPFDKSLEEAL
                     GELAAQCVAACPTGALSFREGKPQLTLPILRVQNLSSQTSR"
     gene            14930..15646
                     /locus_tag="PLD05_01480"
     CDS             14930..15646
                     /locus_tag="PLD05_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOP76126.1"
                     /translation="MSEVKAVSIKPRDKDLSGPPFVNVWWYEFSRVWVRLVARVFFRI
                     RYSGVENIPRTGPVLLVSNHQSHLDPPLIGAGVPRTVSYLARKTLFTNKFFSRLITSY
                     GAIPLDLENPLSGLKEALRRLRCGDAVLIFPEGTRSPDGEIHPFRKGFRVLAVRSQAI
                     IVPLAIEGAYQSWPRSRKFPRPGRIRVHFGRAIGPQDYENLDEEAFIRMVEEKVRQCL
                     ATIRSESGNVRRNDGANVSP"
     gene            complement(15757..18453)
                     /locus_tag="PLD05_01485"
     CDS             complement(15757..18453)
                     /locus_tag="PLD05_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOP76127.1"
                     /translation="MRQNERIPSFVVFLCTVCASDPFRLLSIFRSVSTRSVFHLVFIA
                     FLLTLCGCPAKSRESAGPSQAADQKEPLGTVKAGDSPTTEKGQPVQQATGDRAVSQPT
                     GGVGSQQAAPPLSTETAEEILQKMVEAYGKARSYGDQGRIVMRGVYNGQPFENRTNYL
                     TAFERPNKLRLIAGEGVLICDGQDLWGFVNFLPGQVLKTNAPDKFTLKTLFTDTVLAN
                     ALMQGPGQVYSLVPPPLVLLLADDPLKTFLYRAKPPEVIPPGTLGNSTCDRIRLARLD
                     GDIILWIDRSTRLLVRVDFPTESIRRSLDGGQLQNLAIYAEFPNAVLDADIDPAAFQF
                     QAPPQSRITEELHPHGYDFLGTRPKDFQFVDVDGNPVTLQSLANKPVILEFWSKNHPY
                     SQIVLRMIQAVYEQFKERAAFYAVSIDPPIDTLDSRAVSNQELLNLLQSWSVSIPLLR
                     DPQNDAEKCFDVYIRREAATIPCLVILNAEGVIQAYHLGSAPDLVDRLGATLEKLLSG
                     VNLAEEEKSGFQKMREELARLVAESEKHGLFTFPVEEITAAGTAQAAEATQPQHLRLT
                     PLFQCTAVKSPGNVMIVETSDQESAIYVVEDGQMATELTWEGQVKVQHPLNPIPPDKI
                     NFLRTGVDGQGNRFFVAWSLGSQRVYFFDKNFAAFSRYPADDSPPHDGVADVHLVDLQ
                     GDGTIEAVAGFLGLVGVHAFTLDGKRIWSNRTASAAFRVGIMEPNPAKQRNILATNTT
                     RGSAVEIAHDGQRLREIIVPGRTIAWLVCADLDGDGRSELCGLDPKDFGQVEAFAFNE
                     EGEELWSYKLPKGMTEYPVEQITHGRLLPNQDTQQWVFVGADGSLHIVDINGQLVDQF
                     NVGELITGVAVARFSGKPALIVATPQRVLAWSVEANETKATQ"
     gene            18661..19998
                     /locus_tag="PLD05_01490"
     CDS             18661..19998
                     /locus_tag="PLD05_01490"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOP76128.1"
                     /translation="MRENRERNDRIPHILDEPMDIRISYGTYGVLEIKSTSLVNVVGF
                     PGSDVAPIEDVNAAVREALDHPLELPALARCVTPGDRVVLALRRGTPRGAEILAAVTR
                     YLLPHLSTEGQLTVLRTESDETAGWGDPRPFLSAEEAQALEVRTHRADRQEELTLLAR
                     FRNGRPLRLNRALIDADVVIPVGCTPPGKAWSAYGTSGAVFPAFSDRMSQLAHWRDVV
                     QKARRGKGPLPEHRDRSGREANWLLGTQFAVEVIPGPCGSVLRIVAGATEAARRTARE
                     EYRRWWCPTVDGKAELVIAGVDHNPSHTPWENLAQAAWTAARLVRRGGQIILVTDWDQ
                     GTIPRLIDENSLAEVSGIWSWSDFLQTHGDLAVPFWLLARVRRYARVCLWTAQSLADE
                     SLSGWRERLSLETTSGEAVINRLVNRVDSCTVFSHASLAWPRVSRGATLRGAG"
     gene            20006..>20301
                     /locus_tag="PLD05_01495"
     CDS             20006..>20301
                     /locus_tag="PLD05_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76129.1"
                     /translation="MGKGTENRAAFSDLYCINRFVIADCEMSESIEDILERNLNAVRH
                     RIAVAATRCGRSPQEITMVAVTKYVDLSIIQGLLRLGQRILGESRPQQLWQRAS"
     assembly_gap    20302..20401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20459..21148
                     /locus_tag="PLD05_01500"
     CDS             20459..21148
                     /locus_tag="PLD05_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOP76130.1"
                     /translation="MEAKNLETVPEAVVFDMDGLMFNTEDIYFAVGEELLRRRGKNFT
                     RELSDAMMGRPPQASFEIMIQWHNLSDTWQELAAESEDLFIERIQGRIAPMPGLLELL
                     TALESANIPKAIATSSSRRTLRAVLACFDLEPRFAFTLTAEDVVQGKPYPEIYLKACE
                     RFGLPPHKVVVLEDSEAGCRAAAAAGTITVAVPGSHSARHDFTCATMVISTLRDPRLY
                     QLLRLPRERNS"
     gene            21202..22539
                     /locus_tag="PLD05_01505"
     CDS             21202..22539
                     /locus_tag="PLD05_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOP76131.1"
                     /translation="MGFHWSSRVLGSLVVFSLFIVAGSSLWGETGDELWTAAEMRIEK
                     YRKGDVIITVRDKDGRPVPNAKVRLQQTRHCFLFGCNIFLWGRIEDSQLQTAYNERFA
                     ELFNYATAAFYWASYERQRGRPDHDYTRRLAEWCREKGIVVKGHPLAWNTADPIWLPD
                     DPDEILRLQLARIEDCVSRFRGLIDRWDVVNEVSEYDRPEFVTRRAPKMSAMWKKVGQ
                     MEFTRQCFETARKANPDATLIINDYVTSPKFERVIEQLVDQDGKRLYDVIGIQSHMHG
                     GTWPNERIWEICERFSRFQVPIHFTETTILSGQRGWERPRPWVTTPEGEEYQAKEVVR
                     FYTMLFSHPAVEAITWWDFSDLRAWQGAPAGLVRRDMSPKPAYTELYKLIKQKWWSDE
                     TLSTDSQGRATTRAFAGDFVISAQAPGFSEAKESVKVEWRKPNEFVVVVEKEG"
     gene            complement(22661..23014)
                     /locus_tag="PLD05_01510"
     CDS             complement(22661..23014)
                     /locus_tag="PLD05_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOP76132.1"
                     /translation="MNEQLTNPALDQVPAIYSELADDPDLRDLIQMFVDEMPYRAALY
                     EELFRTGDFEELRRAVHQLKGAAGSYGFTPLTEAATALEQSIRHEAPYEQIRSELNNL
                     LRLCAAARLKPEITA"
     gene            23502..>23710
                     /locus_tag="PLD05_01515"
     CDS             23502..>23710
                     /locus_tag="PLD05_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76133.1"
                     /translation="MKRVTLLGLVALGALVTVLGLNTARADLFLGGACAPACEKPACE
                     QPACEPACEPACEPACRPGLLARIRA"
     assembly_gap    23711..23810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23811..24170)
                     /locus_tag="PLD05_01520"
     CDS             complement(<23811..24170)
                     /locus_tag="PLD05_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOP76134.1"
                     /translation="MSIPSQVRCYDEITQCIGNTPLVRVRRLAAGYGATLLAKLESFN
                     PLWSVKDRIARAMIDAAERQGLIKPDTVIIEPTSGNTGIGLAYVCASRGYKLMVTMPE
                     SMSLERRRLLKAFGVELV"
     gene            complement(24357..>25556)
                     /locus_tag="PLD05_01525"
     CDS             complement(24357..>25556)
                     /locus_tag="PLD05_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOP76135.1"
                     /translation="EGARADAMVTVFPSVTWCAHATLSTGVWPNRHGVLANDYIDRNT
                     KQKVTLLCDPVFDKDQILQTPTIYDVAHAAGLVTAGVLWPVTRNARTLHFSAPDMPGD
                     TAWSDFGTPAWIQELKAAGLPVDSHGRWCREAGGGVPRDWLYVRMARQLLLQHQPNLI
                     MIHLVEPDHVQHRTGPRSDDAYWCASYSDDRIRDLVEAIASSPRAKSTYLVIVSDHGF
                     FPFEKVIQPNVLLRQAGLISVNDGKPVPEKAWCMSQGGAAGIYIFDTQQRDQLMEQLR
                     QAFQSVEGIQAVLTGKEVEALGQKTGQEDPRCPDIWLAAEKGYSFSETTTGDEVVTTR
                     SGLGGTHGYLPDQPDMWAMCVIWGPGIKPGTRLGKVQIVDIAPTIATLLGVELPDADG
                     RALVKPN"
     assembly_gap    25557..25656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25657..26369)
                     /locus_tag="PLD05_01530"
     CDS             complement(<25657..26369)
                     /locus_tag="PLD05_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76136.1"
                     /translation="MDTYCHLCDDHYINLTVNTVLELPAHRETLIHFFERIQKAYPTM
                     GNFYTRDGGEQVVLEDDRDNDSYRWLSVEVRRLSAGYVNPPSVDAAFQYHRMILENAP
                     FHLSLSPLDCEAVDVLVGFDYTYQGNHNQLVADVLGVPPAFERLLEVPSARVVHYEPL
                     LTLALDQDCNLQCRVNIETRTQDYHVRKEEYPEEQISVYVTCRHYGLLPGDNRFLRTL
                     DLLLKTGMTTVDDFVAPMI"
     assembly_gap    26792..26891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26892..27397)
                     /locus_tag="PLD05_01535"
     CDS             complement(<26892..27397)
                     /locus_tag="PLD05_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOP76137.1"
                     /translation="MHKRILPVAILISGSGRTLRDLLKAVDEGRVPIEVRLVIASTPN
                     ASGLQHAEFRCIPAEVIERRDFNSAEEYSAAILRRCQEVGAEYVIMAGFAMLLTITPE
                     YQNRVIGSHPALAPAFCGQGYFGRRVHAAVLEHGVKVTGCTVFFLDQQYNNGPVILQK
                     AVPVEPDDT"
     gene            27691..28242
                     /locus_tag="PLD05_01540"
     CDS             27691..28242
                     /locus_tag="PLD05_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP76138.1"
                     /translation="MREQQGELPPDEEFVRLLSGVQRKLFLFVFTLVPNVVEAEEIVQ
                     STSVVLWQKFSQFRKGEDFLRWACGVARNEVLKHRARASRHEATFSNEFMEQLAETTV
                     PLMEAVDERRQALQRCLEELQPHHRRLLAQRYQEGISTEEIAKNQGKTADAVRRMLHR
                     IRLAIYDCVQKKLASMEWGTSTP"
     gene            28257..29960
                     /locus_tag="PLD05_01545"
     CDS             28257..29960
                     /locus_tag="PLD05_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOP76139.1"
                     /translation="MSSFDREKLTLLIDRVLEGCASREEIRAIEEGARSADAQRFLAE
                     YLLLAGELHWLAIDDDLKDREILARVSLPEGTSDHGDRDLIARTAGSGPWLGGQFRKM
                     GLLVIAVTAVLLIGLISIWLRTGARLPSQGQFSTPVIAQLEKVYPEDSKKIGATIRPG
                     DWVIVREGAAQWQIRGGGSLIAQGSTRFRLLENGQVELESGTIFLRTKESGHLAGVIT
                     PQARYLDRGTAFGVLCDMTRSEVHVLEGEVEVIARRLGLEGSRRVVAGEALACDGRGR
                     TEPIPCRAERFCQMVPEALTVAAYRLMALSDPRLWLYGAFESPRFDGKWHAVLGPTDF
                     QPVWMRGTMSRLDVQQISGYDSQSRAVKLIRGTYSGDTVGVGLQTIDPVVLPRAMTVE
                     MIVRFDGWPSAGENTLACLLATRQDQRRCAVWLGVVPVERESRHLGRLVHLCDGMAPW
                     TETKGTLVPGHWYYVAATFEPERDATRVSTWLADLTEKEPLVCVLKDGQLAGRPVDGY
                     LGIGKGFDDGMSHAYPFPGAIDELAIYNGVLEEDLIRFHLTHLIGTKGQESVLPLPGG
                     P"
     gene            30078..31634
                     /locus_tag="PLD05_01550"
     CDS             30078..31634
                     /locus_tag="PLD05_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOP76140.1"
                     /translation="MGRHGGYQSRRDFLRVTGLSWAIGQAGGIAVPSLLLGEENSGPI
                     DCGPPPKAAPQHRTGGESFPPLPLPVTPLRRSEKKRPPSPPALIGKMALGSVRWITRE
                     GKRVMYRDWMTDPADVNTLLEWTNEKLGIHYRAVETDFAHFSFDPRELPALLFAGHNS
                     FQLNDEVRAKLARYVMDGGTIIGDACCGWNDFAESFRKEMELIFPGRPLRKMLPEEPV
                     FSSYYKLGNFTYQKGDGSQYDDAPCLESIDFGCRSGVIFSPADMTCGWDGHDHPRGLR
                     LTIDLARQVGANLVTYLLGTYQLARFLSTTKVYYEAEAPTRDDFVFAQIIHDGDWDPD
                     PSAVHNLLKFAKDNSTLEVKFKRQNVRLTDPQVANYPLLYMTGHREFVWSDDEVAFLQ
                     RYLKAGGLLLADACCGRMSFDMGFRREIKKVFPNAELERIPPDHPIYHNLFDIQEVDY
                     TPRVREDFGQLNAPELEGISIDGRLAVVYSRFDLGNGWEQFPHPYSYGLKDEAALKIG
                     SNVIVYAITH"
     gene            complement(31664..32674)
                     /locus_tag="PLD05_01555"
     CDS             complement(31664..32674)
                     /locus_tag="PLD05_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOP76141.1"
                     /translation="MKALVLTEYHHLEVRDVPTPKPGPGELLVRVQTCGICGSDVHGL
                     DGSTGRRKPPLIMGHEAAGVVAEVGPEVTRFRSGDRVTFDSTVYCGQCAYCLRGQINL
                     CDNRRVLGVSCDEYRRDGAFAEYVVVPERIVYRLPENVSFQQAAMIEPLSVALHAVDR
                     AAPKIGETAVIFGGGIIGLLILQVLRSAGLDQIITIEPDPHRQQMAKEFAADLVLSPE
                     QFRQTAPPPVDLAFDAVGINETVNGAVNAVAKGGRVVLVGNLQPEVPLPLQKVVTREL
                     TLLGSCASQGDYPRCLNLLAKQAVRVEPLISAVAPLEEGPYWFDQLYYRRQPLMKVIL
                     QP"
     gene            32939..33634
                     /locus_tag="PLD05_01560"
     CDS             32939..33634
                     /locus_tag="PLD05_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76142.1"
                     /translation="MIRVLRILLITTAVVLAVVLVIVGGIWLLVTRTPDFYEKKVPVD
                     PALAKKACDQFLQKTSLFASDLKREGTWYLKVTEEEINGWLAVDLPRNHTQALPRGIW
                     EPRVEIGDDIIRVAAQAEYGHLRGVAHVELQLYSTEANTITVVVKRAALGALPLPLQD
                     LVEQLKKIAARAHWDVRQMQTGGSPTLVITIPKKLDEKGRELELTTIHITPGTIELIG
                     KIKGTPQRSSAVF"
     gene            complement(33610..35088)
                     /locus_tag="PLD05_01565"
     CDS             complement(33610..35088)
                     /locus_tag="PLD05_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP76143.1"
                     /translation="MKSYLRKLAKIAFKAIGLLGLSLAFAYLPPILWGLWEIRLIHDL
                     EGQLAQVPPDQACLILEEFSEMGANGAPALVRALAHPDDTISSHAYRLLWRKLHSRET
                     QATGEQEALVIAEELHRISPFIPPSRKEHLAALLDQLLKWEDGGKNGFKIVKLCESLL
                     ERKLVDNRPVGEESKAAPGPNTAESSNGATVLRNSPPTMLQISGDPLPLRETGPNAEA
                     TASAVSPITEGMNTDHSSPAVVDSWQVSTSDLAAWREVFGGPLAQLAIYEKEVSTSEG
                     TSRSFGPGSMATYLAPQGTQDGGWKSVTREITPDRNETSAGPTLDESSSRPSSGKPSD
                     QPPRSISNRPDTTSAEKGATWATLAPLFAQLSGSPAEADRALQTLISLGITRPTIEVG
                     RMAFHPDSARRRQAIHLLWNLPGVDPLPFLLGLAQDSDPIVRREALAVLATTTNPAIH
                     SFVRDAIGRDSDPQVRALLDKIDSTYRAVSTTTTHQNTAEER"
     gene            35522..36418
                     /locus_tag="PLD05_01570"
     CDS             35522..36418
                     /locus_tag="PLD05_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76144.1"
                     /translation="MSFFPILFILACLSVFAILPNFASGQGQYGASRGTSEAVSVSAK
                     EEKQQAINRIPLTELDPAWRTRVLDVAARAALFRRMSSQMIECEPEFYRFLVEHPEVV
                     VAMWQALGVTKLKLQIVGPQKYRLNDGAGTTAVVEYLYSSPNQHVVYSEGSYSGPLML
                     RPVEGRVLVVLTSNYLREATGTPYVSAQMDLFIQVDRAGIELITKALHPLLGGMAETN
                     YQQTMAFVGSVYRTAVVRPQSLERLASQLTQLEGNTQQDFLNWIARIGNDSGTTYDRA
                     AMITPPDGIHERALQPESTIRQ"
     gene            complement(36484..40035)
                     /locus_tag="PLD05_01575"
     CDS             complement(36484..40035)
                     /locus_tag="PLD05_01575"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HOP76145.1"
                     /translation="MTKLPGDRHSSATAACVSQQSSSLWSRSFLALLVTQFTVALNDN
                     ILRWLFIPIGKDLVLEQLKESPDVARSVGSFIFLLPFILLAGWAGATSDRFSKRTVII
                     GAKVAEIIVMTLAIVAILTSNVWAMAVVLFLAGTHSAFFSPSKYGSIPEIVGPKLISA
                     ANGVIGLTTMIAVIVGGSVGNLLYSLTTIRDPALAQARGLGPGQYRWWINAVVLLGVA
                     TLGWLTSLAIKRLPAADPQRRIPLNPFLQCYYDLKELSRHRVLLLAAIGSTYFWTLGL
                     LMQLTIDKLAVPELVGPYGQAYVGPMLGILTLGIGLGSMLAGVWSHGRIERGLVPLGA
                     LGITVISLAFAFLPAGTGHWNSSPYFLACGLLFLLGAAAGLYDIPLISILQFESPAHE
                     RGRILAAYNFLSFTGMIMGSVIYWFLGSFLGLSARQMFVCAGIVTFPVFLIILYLTPI
                     DVIRVLFGWLMRWLYRFRVVGLENVPADGPAIIVSNHLSWLDGMMIMLAMPRPIRMIA
                     HLKYINAPIIRDLAKRAGVIAILPGAKSVALGIREARQALEDGELVGIFPEGGMTRTG
                     QIRGFQPGFLKIARGLDVPIIPVYIDGIWGSIFTYSGGRYFWKWPGLRPLRVTLYVGQ
                     PLRNIREAWQAHLALASLGAEVVSQKNTIDHIPPRRFLRTARSLLWGRKIADSTGIEL
                     TGGRLLIASLALRRFLRRHYLKPDETQVGVLLPSSVAGVVTNAALALDRRIAVNLNYT
                     LSEDLINYCIRSAGVKHVLTSRRMLERFPFKLEATVVFLEDIYSQLELADKLISAFQA
                     YLLPAWVLERLLGLHRIDPDDVLTLIFTSGSTGRPKGVMLTHRNIGSNVAAFNQILHL
                     RRSDVLCGILPFFHSFGYTTTLWTALQLKPMVVYHFSPLEPRPIGQICRKYKATILVA
                     TPTFLRNYVRRCEPEDFAHLEVVITGAEKLPPEVADAFEQKFGVRPWEGYGLTETSPV
                     VSSNVPPSRVREPWFVSAKQGTVGRPIPGVAVKVVDVETGEDLGVGKQGMLLVKGPNV
                     MKGYWNDPEQTAKVIKDGWYITGDLAQLDEDGFITITGRLSRFSKIAGEMVPHLAVEE
                     AIGRIEMTGDQETITFAVTGVPDEKKGERLVVLYTQLQHTPSEICRALQSNGLPPLWV
                     PDTEHFYRVDQIPVLGSGKLDLKALKEMAQQVAGGRD"
     gene            40369..42441
                     /gene="metG"
                     /locus_tag="PLD05_01580"
     CDS             40369..42441
                     /gene="metG"
                     /locus_tag="PLD05_01580"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOP76146.1"
                     /translation="MSRKILVTSALPYANGPIHIGHLVEYIQTDIWVRFQKLRGNRCI
                     YICADDTHGTAIMISARREGIPEEEFIQRMYEAHVRDFAGFDIEFDNYGSTHSPENRE
                     ICEVFWRALRQAGLVIEKEVTQLFDPVAGVFLADRFVKGTCPNPACHAPDRYGDSCDR
                     CGHTHSPAELIDPVSTISGAKPELRTARHLFVQIERLHDFLEEWTQTGDHLQPEIANY
                     LKAHFLSEPLRDWDVSRPAPYFGFEIPDSPGNYWYVWFDAPIGYIGSTKQWCDRTGES
                     LDDWWRNPETEVHHFIGKDITYFHCLFWPAMLKTAGFNLPRKIHIHGFLTVNGEKMSK
                     SKGTFIKAETYLRHLDPSYLRYYYAAKLSSRVEDIDLNLEEFVSKVNTDLVGNIVNLA
                     SRTARFLEGQNLSPTYPDDSGLFAQAAADGQAIAEAYEKCDFGKAMRLILAAGDRANQ
                     FIERAAPWNLKKDPAKQSQLRDVCTIGLNLFRQLVIYLSPVLPRLAAQTGKLLNDPVS
                     SWDQSQVPLVGNRVAPFEHMMTRVPKEKLAAVVEESREVEVASGGGEGNRPAEGVSPE
                     TADTDETLQREPLLTSTVTIDDFAKIDLRVARVLAAEEVPGADKLLKLTLSLGGNHTR
                     TVFAGIKKAYKPGDLVGRLVILVANLAPRKMRFGTSEGMILAAGWGDDQVFLLAPDQG
                     AKPGHRVH"
BASE COUNT         9139 a        10890 c        12520 g         9640 t
ORIGIN      
        1 agtctgtgac atttggcgag cgaattaaga agggagaatt accctatccg gttccaaaag
       61 agttgatgga aaactaacat ttcgtgcggc cggcggcgag cgatagccag gttggtggtt
      121 gagccttggc taatcgcagg tcacgagtgc cgggatttcc gggtgcttgg ggccgattcg
      181 ttaatggccc catctttccc gtaaacgcga cggaaggcac aatactctct gcctcggata
      241 cggtgtcaca aaccaagaaa cgggcggttt taagttctga ggtaattgtt ttttggcaac
      301 cgcatgatgt ctgaccttgg tatggcactc tcttgatcac ggacagcgac ctagatggga
      361 aggactttgt acggcctttt ttctatcaat gtttggaagg agtaggacga tgcgacgcct
      421 tttggtgaag atgcctgtgg tgttaacgct agtggtcgtc atggtcagct tgcaagccgg
      481 tggaagtttc tgttttggtc aaatgtataa agatctgcac gcgaaattgg aaaaaaaggt
      541 ctataaggat tccgagggtg ggacgcttcc ctatcggctg tggaagccag cggattatga
      601 cccggccaag aagtatcctt tgatcctttt tctccatggg gcgggcgaac gtggagagga
      661 taacgaggcc caactgaaga acgatgaatt cctcacgttg gtcttagatc ccgagcatcc
      721 agcaatcttg attgctccgc agtgcccgag caaattatgg tggtcggcca tccgccggcc
      781 ttgggatcaa ccggagtcgg gaaatcctga accaacccca cccatgaaac tcgtcatggg
      841 cctattggac gaggtgcgga aagaactgag tgtcgatcca gaccggtgtt acgttaccgg
      901 actgtcgatg gggggattcg gaacatttga cctcttgttg cgtcgcccca acgattttgc
      961 cgcggcggta ccaatttgtg gtggcgggaa tgtggaacgg gccgccgaaa ttgcccatat
     1021 tcccatgtgg attttccacg gtggtgccga tccggttgtg cccgtcaaac tctcccgcga
     1081 catggtggaa gccctaaaaa aggccggggg agaggtgcgt tacaccgaat atccaggcgt
     1141 tggccataat tcttggtcaa aggcctatca ggagccggag cttcgtaagt ggctcttcga
     1201 acaggtccgc aagaagaaag acggctgacg tttttgctgg cacggctgaa ggcgggtacg
     1261 ttgccaacac ggacactaat tcgctgaggc ccaaggcgaa caattccatc agagaaagga
     1321 ctgtgatcat ggctgagcag tcacgaaatg tccaaggcgg ttcccgtacg gctcggcacg
     1381 gtgcgaatcg gcgccagttc ttggccgccg ggggtgcggt ggcagtttcc attttggaac
     1441 cgtccttgat ctttggcaca caagcaaacg aaaaggttcg actggggctg attggctgcg
     1501 gaaatcgcgg tcgttggatt gcgagactat ttcgggacca cgggggatat gtggtttcgg
     1561 cggtccatga ctattttccc agtcgagcca aggccgtagg aggcgagttt gcgattacgg
     1621 aggatcgatg ttacagcggc ctgttgggct accgccgaat gctggatgcg ggagggattg
     1681 acgccgttgt catcgaaagc cccccatatt ttcatcccga gcaagccgtt gcggctgtgg
     1741 aagctggtgt tcacgtcttt ttggccaagc cggtggccgt ggatgtgatg ggatgtcaga
     1801 cgattcagac tgccggggag aaagcaaccg ccaagaagct cgttttcctg gtggattttc
     1861 aaacgcggac aagtcccctg taccaggagg ccgtccggcg ggtaagagag ggggatatcg
     1921 gaacgatcgt cagcggcgag gccgtttatt attgcggacc cacttgggtc gtgccggaag
     1981 attttgatcc caaaaaccct gaagatttac tgcgggcgtg gggggcattt cgggtgttgt
     2041 cgggcgacgt catcaccgag caaaacatcc acgccctcga tgttgccacg tggtttttgg
     2101 acgctgctcc gttgcgcgcg tatgggatgg ggggccggaa ggggaaaacc ggtccgggcg
     2161 actgctggga tcacttttcg gtcatcttta cgtttcccaa tgatgtgctg gtcacatttg
     2221 cctcaaaaca atacggtgcg ggctatgacg atattggctg ccgcatcttt gggacagaag
     2281 gcacggccga cacgcactat tttgggaccg tcagcatcca cgggaaaaag ccattcccgg
     2341 gtggttcgac gggtagcctt tacgccgacg gtgccaaggc taacatcaag acgttttatg
     2401 atgccatcaa gaatggtgac ttcagcaatc ccaccgtggc acccagcgtc cgcagcaatt
     2461 tgacgacgat cctgggacgg atggcagcgt atcaaaaatc ggaagtcacg tgggatcaga
     2521 tgctggcttc ccaggaacgt tgggagtttg acgtcaccgg actgaaagaa tgattgggga
     2581 cgccgtgacg gaccttgtgc tgcatcggac ccgtgccgca tggttaacgt tgggcattcc
     2641 cattgtcacg gcccttgccg gcgtggcggc cattgtttat tggttggtcg cggtgcccga
     2701 accaaaactc agtccgcgag tccccggtct cgatcgtccg ctgcagcgac gaaccggtac
     2761 tacggtgaaa cccaagttga cgggcaccct tgtgcgaggc gatggatcac cgtcaaacat
     2821 tccgggcgaa tggccccgat ttcgtggacc gaaactggac gccatttggg aggacggaac
     2881 cgttaccctg gcccggtcct gggatgccga tggcccgccg cgagtttggt cgattgcggt
     2941 gggagaaggc tttgccgccg ccgccatccg cgatgggcga gtctatctga ttgattacga
     3001 ccgggaaaag gagcaggacg tggtgcggtg cctctccctg gatgatggac gcgacatctg
     3061 gcagtttcgc taccctaata cggtcaaaag aaaccatggc atgtcgcgga caattccaac
     3121 cgtgacggaa aagtatgtgg tttccattgg accaaagtgc catgtgacct gccttgaccg
     3181 aaccaccggg gagtcgcgtt ggctgatcga tatggttgac cgatggggga cgacggtgcc
     3241 gccgtggtac accggccagt gtccctttgt cgatcaggat tgtgttattt tagccccagg
     3301 cgggcccgag gccttgatcg tggcggtgga tttggaaacc ggggaggagc gttggcggac
     3361 ccccaatccc cgcaattgga aaatgactca ttcctcgatc attcccatgg aatcctgtgg
     3421 gcatcgaatg tatgtctatt gtgcaagcgg gggtgttgtg ggggtggatg cggccacagg
     3481 ggcaattctt tgggaaactg cagaatggaa aatcagtttg gcgactgtcc cttcgcccct
     3541 gccgctacct ggcgatcgca tttttctctg cggcggctat gatgcaggtg ccctgatgtt
     3601 gcaattgagg aaagaagggg agaagatcac cccggaggtt ctttaccggc taccgccaca
     3661 ggaatttggg tcaacccagc agacacctgt tttcttcgca gggtgcatct ttggggttcg
     3721 tgaaaaagac aaacaaatgg tctgcctcga tttggatgga aaggtccgtt ggtcgagtgg
     3781 gtcgcaatat cggtttggcc tcgggccgta tatgattgct aatgggttgc tgttggcttt
     3841 ggatgatgac ggcacgttga ccctggccga ggcgtcgccg gaggctttca agccgatagc
     3901 ccgcgccaga gtcttggacg gtcacgattg ttgggggccg atggccatcg taaaaggacg
     3961 tttaatcatt cgaagcatga ccgaaatggt ttgtcttgat ttaacctccc cgtagccatc
     4021 gtaaggcctg cgcaaaggtc aatccatgaa atatcgggtt ccagacgatc gccacaccgg
     4081 cttcagcaga tacacgtccc agatggaccg tggatcatta ccatccggga tccaacggtg
     4141 ggaactgctt ctaatggttg ttgtcgttgt cgcggccgcc gctgtgggcg cggttgcggt
     4201 gtggcagtat cgtaaccact tgcgaaaccc ttcccgtgac ttgaaagcag agcgattttc
     4261 ctattcactc gatctttacc aggagattcc acctgagcga atcggcttcg aaccggcgtg
     4321 gcagcttgcc attcccggag accagatccg gcgggtgacc gtcttccaaa acacggcatg
     4381 ggtgtgtgcc gatcaatcgt tgattcccgt ggcccttact tcggaaggtg gcgctgtggg
     4441 atcccccatg agcgtgccgg tgacaccata ctgcctcgcc gtagctgaat ggccggacca
     4501 agataaccgc gcggagcggt actttgtcgt aggaggtggg aatcaggtgc tggtcatccg
     4561 agaaacaggg gccgtcgttg gtgaatggag tttcttcggt cggaaggcca atatcaccga
     4621 tttggccatt gctgatgaag acgtttttct tgccgacgca ggtaaccggg ttgtccatcg
     4681 ggtggaccgt tttggcgaga aggttgccga gatcggccac cgcgacgcag aacggggaat
     4741 cctagggttt atcatcccga gcccatattt ttcgcttgcc tggaatgggg acggcctcct
     4801 tcgcgtcgtt aaccccggcg ttcatcgcat tgagttctat agccccgacg gggatctgga
     4861 gactttctgg ggtaaagcgg gactggatga ggcgggcttt tgcggatgct gtaatccggc
     4921 gaggatcgcc ttgctgatgg atgggcgaat tgtcacagtt gaaaagggaa ttccgcgaat
     4981 taaggtttat tcgccccacg gtgacttcat ctgctgggtc gtattaccga gctattttac
     5041 tggttcggca caatggaagg agacccggga tcaagagcga ttaccggtcc tcgatgtggg
     5101 cgtctttcag gtagtcgagg atcgagggga aggcgaagaa tggatcgtga ttcttgatcc
     5161 cgcgagaaag gtcctcgagg catttcgacc caagcggccg gaagagccaa acgacaagac
     5221 gccgagtagc ggagcggcgt ccgctggaac gccggatgag gcgcattgag ttaggtgagg
     5281 tgtgaacgga acggtttaga ccctcgatgc cccaaaaacg aaagaatggt tacagcgatg
     5341 acgagagagg tggtaacact gcgaatttcc cgacgtgaac tagtccgtgc gggacttcgg
     5401 gctttggttc tgggtgcact cgccgggatg gtgtttttct tagggcgacg aacggggcgt
     5461 tgcccgtcgg gcgatctggc acgctgcgat cgttgtcctt ggggggaagg ctgtgaccgt
     5521 cggcccatga agcaggtagg gggacaggcg tttctggtgg gcacgagagg tgagacttac
     5581 agtggagggc ggtcaccctg actcacttcg gtgatttctg agaaagaagg agccacaacc
     5641 cgtgagtaac gctcaatcga ggcagcgcat ccctcggcgg gaaattttgc gggaaggcgt
     5701 tcagatggcc gctttcctgg gtgtgggtgg cttggtcggt tttgtggcgg gtaggaaggc
     5761 cacgtccgaa cccctggtgt ggcaaattga cccctataaa tgcatcgcct gtggcaattg
     5821 cgccacacat tgtgtgctgg atccgtcggc ggtgaagtgc gtgaacagtt ttcccatctg
     5881 cggctactgc gatttgtgta ctggttactt tccccctgag ccggcggcac tgaatacggg
     5941 tgccgaaaac caactgtgcc caacgggcgc tattatccgt aaattggtgg aggaacccta
     6001 ctatgagtac accatcgatg aagagctttg catcgcctgt gggaaatgtg tgatcggttg
     6061 tgcggcattt ggcaatgggt cactgtatct tcaagtccgg catgatcgat gcctcaattg
     6121 ccacgaatgc tcgattgcga ttgcctgtcc gtcgcaagcc tttgtccgag tgcccgcctc
     6181 tgagccatat cttctgaaac ataagtacaa gcccgccgct cctgcctgat gtgtcgaact
     6241 gtgttccttc gggtaggcag gtcaggcgat ataggttaag gcgaaagact gaaattcatg
     6301 acgactgtga gtgatcaaga attgcataag cacctggagc cgtgttgtgc caaagccccg
     6361 cggccaaggc ggagaatgag tcgcttttcg attgggctgt tggccatcat acttgctctt
     6421 gtttggacat acgccagccg tgcggtggaa cgatttccgc cccctgactt tacgtctcat
     6481 cagttgccgg agacaccgca accgccaccg cccgctccgt ggtgggagac gattgatgtc
     6541 gttcttcttg tcacggcttt ggttttggcg acttattttg ctttggccaa gcgatcacgg
     6601 aatgccacac tcgttttgac aggtttttca ttgttttggt ttggctttca tcggcaggga
     6661 tgtgtctgtc caattggggc gatccaagat gtggcccagg ggttgtttga ttctgcacac
     6721 gccattccct ggagcatctt agtattcttt agtttgcctc ttgtatttgc cctgtttttt
     6781 ggacgggtat tttgcgctgc cgtctgcccg ctgggagcga ttcaagagat tgccgcgatc
     6841 cgtccccaca agctccccga ttggctagag cagatgttgg ggttgttccc ttgggtttac
     6901 ctgggattgg ccgtgctcta tgcggcgacc gggacggcct atgtgatctg cgaatacgat
     6961 ccatttgtgg ccttctttcg ccgcagtggt gccacctcga tgcttgtttt tgggggtgcg
     7021 ctattggcga tcgggatctt cgtagggcgg ccatactgcc gttttctttg tccgtacgga
     7081 gtgatccttg gccttttcgc caaattggcc cggtggcacg tgcggattcc tccgacggag
     7141 tgtattcgct gccgtttgtg tgaagatgca tgcccctata acgccattgt ggcgcccgtt
     7201 ccccctttga cccgatcgga acgtgccccg gcccgacgcc gcttgctttt ggccatggcg
     7261 gtcttgccca tctggttggc actgggcggt gggatcggat acgctcttca tccgataatg
     7321 gcccaacttg atcctcaggt ccgcttggcc cggtgggtac ggtctaaaaa cattgatccc
     7381 aacaacaagt ttgccgtgga tgcggtgacg gcctttcata acacgggcgc aagtgaagca
     7441 tcactgtatc aagccgctgc ggagcgactg cgaaaatttg gcgtggggag cgcgattttt
     7501 gggctctggg ttggcttggt gtttggtctg aaatgggttc acacctctac gcgtcccagc
     7561 cgccaagagt atcttcccga cccgcgccgc tgcgttgcct gcggccggtg tttttggtac
     7621 tgccccgtgg agcaagtcag gcgcggatgg atcattgagg acgaattgtt acaactgcct
     7681 aaagagcgag cgccattggt ttcatccgtg gcaaatgacg aatgagaggg tggtgcatcc
     7741 gtttttgttt ggaaattcat cacgtgaggc attggatacc gggtggtcat gatgagtcaa
     7801 aggcgtgaag agtcaaatgt accagataag ggtgaagctc agttggcccc cgcaaacttg
     7861 agggtggaga ctccaggagc gttggtggct cctctgccgg gttttttggc agcactccgg
     7921 acggcaattg tggcagccat cttcgtgttt gttgtagtcg tgctccttgt ctggaacgct
     7981 ctgcatcagg tcaacaaaga ttctctcgat tcaccacaat atgcggaatt gcagaaaaag
     8041 ttgagcctgg acccccaaaa cgaggagctg aaacagcaaa tccgagagct cgatttgcag
     8101 cttcgtcaag cctatttccg cagtcggacg ttcaatcgct ggggaaccat gcttctcatc
     8161 gcgggtctga ttgtctttgc ggtagcggcc cgaatagctt ctggattacg ccgtcccctg
     8221 ccacgtccca cggcaacgga gatatgggat gaatccttcg atcgcgccac gcggctccgg
     8281 cggtttgcca ttttggggat ggctggtctc ctagtcgctg tcgcatgtgt cttttgggcc
     8341 gtggggcgat cggtgataaa ggatacgctg gcagtgcggg aagccgcaga ggttccccca
     8401 cgcgaggtgg ccgccgtggt cacctccccg accgagtcaa ctgttgtcca gaatggttcc
     8461 cgaggtatgg ggagctcgca acaggggaag gccattttta ccgaagagta tcgacgttac
     8521 tggccacgat tccgggggcc agatgggtca ggcatttcgc ctcatcccaa tccacctgtg
     8581 agctggaacg gcaagaccgg cgagggtgtc ctctggaaag tggcagtgga tctccccggc
     8641 aacaattccc cagtggtttg ggccaatcgt gtgtttttaa cgggggccac ggaggagaga
     8701 cgctgcgttt attgcttcga tgccgattcc ggaaagctgc tttggcagca ggatattccg
     8761 gcaacgccgg aaagtgagaa caaaaaaccc aaggtcatga aagacacggg cttcgccgcc
     8821 cccacggtgg cgacggatgg tcaacgagtg tatgcgattt ttgccaacgg cgacttggcc
     8881 gccctggatt ttgagggcaa gttgctgtgg actaagggat ttggagttcc cgataacgcc
     8941 tatggccatg ccagttcttt gtgcacctac ggtgacctgg tcatcgtcca attggatcaa
     9001 gggacccccg acgatgatct ttcaatctta gcagccctcg aaggcgcgag tgggaacgtg
     9061 aagtgggagg tgaaacgacc aactgggaat gcttggacaa gtccgatcat tgtctcgagt
     9121 gcgggcaagg atcagctcat caccgtggcc gatccatttg ttatttccta cgacccgctt
     9181 accgggaaag agtggtggcg attcgaggga acaactggcg attgtggccc atcaccggta
     9241 gttcatcgtg atcttgtgat cgcaggtggt gagtattctt attacatgtt cgccattcgg
     9301 gcggacggca caggtgatgt tacagagacg cataaggtgt gggaaggcga agaggccctg
     9361 cccgatgcct gtagcccgct ggcctttgac cattacgtgt tgtggatgtc ctcgggcgcg
     9421 gtcctggcgt gttatgacat cgaaaagggc gagatgcttt gggagcatga atttcctgaa
     9481 aacacgtttt atgcctcgcc cgctttggcc gaccaaaagg tctatctgtt ttccaaagag
     9541 ggaccgtgtt ttgtaggcgt ggtcacggat gaggagttca aaatcgagca cacgaatgag
     9601 cttggtgaga aatgtgtcac cagtccggcc ttccaaccgg ggcgtatcta tgtccgaggt
     9661 gaagaacatt tgttttgcct ggggcagtaa aatagacaat agcatatgac aaaaatggcc
     9721 tggcttggtc gcacggattt cagttgggcc atgtccttcc gcaaagctgt ttaaacgacc
     9781 agaagaggaa tagccatatc gatatcactg attcggggga tttctgatcg ccaatcgttc
     9841 ccagttgggg aaatgaccgc gaacgcggtt gatcggggaa cggccaccgt ggatttgaac
     9901 attgtcgatg agattgtcga gcgttgtggt cggaagccgg aggcagcaat accgattctc
     9961 caggcgatcc aggcccattt ccggtatttg ccgcaggagg ccttggaacg agtttgccaa
    10021 ctgacggaaa tacgtccgtc tcagttgatc ggggtggcca cgttctatgg ccagtttcgt
    10081 tttcgaccgg tggggcgtca tatcatccat gtttgtcatg gtacggcctg ccacgtgaaa
    10141 ggggccgagc ggattcaaga ggccctggaa cggcatttgg ggctacgacc gggtgaggac
    10201 accgatccgg aggggctctt cacggtggaa aaggtcgcct gcctgggttg ttgcaccttg
    10261 gccccggtga ttcagattga tcggtccacc tatgggcgat tgaccccggc gaagataccg
    10321 cgggtattgg cggactttct ccacgcccag agcaacggat cacacccttc tcgtgggcca
    10381 tcggctggag actttgagga gggcgaagta cgggtcggcc ttggctcctg ttgccaggcc
    10441 caaggaagcg cggaggttta tcgacggttt gaagaattgg tgactctcgc cgcggtaccg
    10501 atttctatca agcgggtcgg gtgcgtggga atgtgccacc aaacgccgct ggtggaagtt
    10561 gtgccacggc gtgggaagtc ccaaatctat gccaaagttg agccagacga tgtgccggcc
    10621 atcgttcatc gacacttccg cccgcgtaaa ctttggnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnaagg ctacaggcga gccaaaatac gtcacgtgca acggggatga
    10801 gggtgacccc ggtgccttta tggatcgcat gatcctggag tcgtttccct accgtgtcat
    10861 cgagggtatg cttattgccg ccaaggcggt tggggcacac gagggaatct tctacatccg
    10921 cgccgagtat ccgttagcgg tagagcgggt gcgggcggca attcacatct gtgaagagcg
    10981 gggtatttta ggcgatcggg tgttgggtac ggattttcgc ttccacctcc gggtcaatga
    11041 aggggcgggg gcgttcgtgt gtggtgagga aacggcattg atcgcgtcga tggaagggcg
    11101 acgggggatg ccccgactca ggccaccgta tcctgccgag ttcggttttg gcggcagacc
    11161 taccctggtc aacaacgttg agactttcgc cctcgtcccc tggattattc ggcatggtgc
    11221 cgaacgattt gcccgtttag ggacggccac aagcaagggg acgaaggtgt tcgccctggc
    11281 gggcaaggtt cggcggggtg ggctgatcga agttccgatg ggtatcacgc tgcgggaaat
    11341 tatcgaggaa atcggtggcg gagcgctccc cgggcgaagg attaaagccg tccaggtcgg
    11401 tggtccctcg ggcggctgtg tgtctgaggc ccttttcgat actccggtgg attatgaggc
    11461 cctcacggcg accggggcca tcatggggtc aggtggactc attgtgctgg ataataccga
    11521 ctgcatggtg gatatcgccc ggtattttct ccggtttacc caagatcagt cgtgtggaaa
    11581 gtgtacactt tgccgcattg gaacacgtcg gatgctcgag attctggagc ggatttgtaa
    11641 cggcgaagga cgagcgggcg atctggaggt tttggaagac ttagcgcgaa ccgtcagtgc
    11701 cggaagcctt tgcggtttgg gcaagacggc tcctaacccc gtcctgacga cattgcgcta
    11761 tttccgcgag gaatacgagg cccacctccg aggcgagtgt ccagccggaa aatgctctcc
    11821 cttgatccgg tatgtgattg ccgaccaatg tatcggctgc acgctttgtg cccagaattg
    11881 tccggtgggt gcgatacccc ttcggcccta tcggcgtcat gcggttgatg atgctaagtg
    11941 cacccgctgt gacacatgtc gattggtatg tcccataggt gcggtggttg tggaatccgg
    12001 cgggcgcgtc gtggcctatg ggcccatggc ccagacgaat tcaacaacgt cgccgggcgg
    12061 accaacttcg gcaagtgggg ggacgtgcgc aacccttccc cagtttgtcc ccttatctga
    12121 gggtcgaagc gggattgtcg ctaccgcaac cagtcaaaac agtgggagag cactatgaat
    12181 gaggctcact gccgcgtgac catcgacggc cggattatcg aggtccctcg ggggacaaca
    12241 gtgctccagg ctgcccgaca gttgggcctg gatatcccca cactttgtta tctcgagggg
    12301 ttgaaacctc aaacctcttg cctcgtgtgt acagtcaaag tcattgagaa tggtcgtgcc
    12361 cgcatgatgc cagcctgtgg gacaccagtc tccgatggga tggtcgtgga aagcgagacg
    12421 gcagaggttg cccatgtccg tcgcagggca ttggaattga ttctgagcga tcacgttggc
    12481 gactgctttg ccccgtgcca ttatgcctgc ccggcgcgga tggatatccc gactgtcttg
    12541 cgacatatcc aggtgggtca gctaagcgaa gcgattgcca ccattaagcg agctgtggct
    12601 atcccggcca cattgagtta tgtttgtccg cggccgtgtg aaaagggatg ccggcgacat
    12661 tatctggatg agccggtcat gatttgccag ctcagccgat ttgttgcaga aaaagaccta
    12721 acaagcgacg ccccttactc cccgccatgt caacctaatt cgggtcgcgc cgttgccgtg
    12781 gtgggtgctg gtccaacagg cttgtcggcc gccttttatc tgaggcaggc gggccatcag
    12841 gtgacaatct tcgatagcgc tccgcaaccc ggtgggcgac ttcggcagat gattggtgaa
    12901 gatgcggggc gcgtgcccat tgacatcctc gacgctgaga tcgccttgat cctacgatcg
    12961 ggcgtggaat accggggcga gacgccgctt gtcaccagtg agcagtccgg tctgacgctc
    13021 actttgctca cccggcagtt cgacgcggtg ctcttggcct ttggagaaca atcgcaaggg
    13081 ttctaccggg cgatagggtt ggcctgcgat aatcgggggc tggttgtcga tcggggtacg
    13141 tttcaaacgt cacttccgca agtgtttgcg gcgggaagtg ccatccgcgg tcgggtgtcg
    13201 gttgcgagaa gttgcgcgga cggtaaatcg gtggctgagt gtatcgacgc ctttttgcgc
    13261 gagggtcatc ctcgtaatgt gacagaacca tttggcgtcc gcatgggccg actggaaaaa
    13321 gaggaattgg acagactagc tcaagtggcg gcgcgatccg gcaaggaaac gccgccgcag
    13381 gagtgggata ttgaaacggc tcgtgaacaa gctggccggt gcctgcattg cgattgtcga
    13441 gcattgccgg tgtgccgttt gcggcagtac gccgagcgtt acggtgtgga ccccaatcgc
    13501 taccgtgctc aacgggcgag gttcgaaatc gtctctcaac cgtccggcgt catttttgaa
    13561 ccgggaaaat gtatcctttg cgggatatgc gtggagttga ccgaatcagc ccgcgcgcct
    13621 ttaggtttaa cttttattgg aaggggcttc gatgtgcgaa tcggcgtgcc ttttgataag
    13681 tcgctcgagg aggccttggg cgagctggcg gcccagtgtg tggcggcttg tcccacgggt
    13741 gccctatcgt ttcgagaagg aaaaccacag ttgacgttgc ccatcctccg cgtacagaat
    13801 ctttcttcgc agacctcgcg gtaaattgcc aaccgcccgg aatggagcaa attaagagcc
    13861 tatcccacaa tcttccctga cccgttcggc gggaaagggg tcgggggtga gggtggctcg
    13921 aatcgctgcc ggttttctca atcgattcca acgtttgctt cccctcaccc caatcctctc
    13981 ccggcgggag aaggggactt tttggtataa gctttaagcc acctcatcac tgccagccag
    14041 ctgcaggaag agcccctaag acgtttgtgc cctaaaataa aaaaggaggc cgtgggccgt
    14101 ctggcagtgt cctcccaagg tgcctctcca actagtcacg cccggcaggg tcatcgcgcg
    14161 gagaaagacg agctttccgc gctaccggac tgactttcgt tgcacaacac tgccagacac
    14221 gctggaccag cgccaaaccc gggtgtacct cttcgtcagg ggaagatcgg tgaaccgttc
    14281 atcaaaggtg ctgaggcgcc gaccgcgctc ggcccatcgg ccaatagcat ctgcaattga
    14341 agaaatcctc ccttagtttc ttctcgccgt ggtacctaaa ttgtccccgg accaaagcga
    14401 gtcccgttgt ttaccaccag cgaccgtgcc ggtgttagca aggcctccgg tgaaacaacc
    14461 cgaactgcga ggatttcctg agagattaac gttggggtga atccctgaat tcgggtgacg
    14521 tccgtttcag aatctgatga taatgtacac acgagaatcc aaaatgcaag acggtgaatc
    14581 cattttcttg gtgggaacct cccataccgg tcatctccca ggaacaggac cggtacacat
    14641 cgcattcctt gcctaaatag cccagaatat atattcttgc attcgcaggg tgttcggggc
    14701 cctccttatt ttttcaagga gggtgaccaa gtgccgcatg tccgcgagag ttcgcacatg
    14761 cgcactcgga tagagggcag ggcttgctcc tgttgtctca aacggaccct ttcgccggta
    14821 tcttgttggg tttacactcg acaaaaaacc gtacatacac aagaggctgg ttactgctgc
    14881 ctaaaaagca gtgctcgtga gtgatgacac ctggagggtg ataacagttg tgtccgaagt
    14941 caaggctgta tcgataaaac ctcgagataa ggatctttct ggtccaccat ttgttaacgt
    15001 gtggtggtac gaattcagcc gggtttgggt caggctggtg gctcgggtgt ttttcaggat
    15061 tcgttattca ggtgtcgaga atattccccg cactgggccc gtgctgctgg tctccaatca
    15121 tcagagccac ctcgacccac cactgattgg agcaggtgtt ccgcggacgg tgagttatct
    15181 tgcccgtaag acgctcttca ccaacaagtt cttttcgcgg ctgattacct cttacggtgc
    15241 catccccctc gatctggaga atcccctgag cggcttgaaa gaggccctgc ggaggcttcg
    15301 gtgtggggac gccgtgttaa tttttccgga ggggacgcgt tctcccgacg gggaaatcca
    15361 tccctttcgg aaaggatttc gcgtcctggc ggttcgatct caggccataa tcgtgccact
    15421 ggcgattgag ggggcctatc aatcgtggcc aaggtcgcgg aagtttcctc gccctggtcg
    15481 catacgggtt cactttggcc gggcaattgg tccccaagac tacgaaaact tagatgaaga
    15541 agcctttatc cgcatggttg aagaaaaagt tcggcaatgc ctagcgacaa ttcgaagtga
    15601 atcgggcaac gtgcgccgga acgatggtgc gaacgtctca ccttaaggcg cagttttcgt
    15661 gaaacaaatc gcacctggtc acaaaatggc gattgccgca acctcgtgac aacacgtgtc
    15721 cgagctttag cacattcgag tatcgccttc ctcggatcac tgggtagctt ttgtttcgtt
    15781 tgcctcgact gaccatgcga ggactctttg gggcgttgct acgatgaggg ccggcttccc
    15841 tgagaatcgc gcgaccgcca cgcccgtgat gagttcaccc acgttaaatt gatcgaccaa
    15901 ttgtccgttg atgtccacaa tgtgaagtga accatcggca ccaacaaaga cccattgttg
    15961 ggtatcctga tttggcagga ggcgtccatg ggtgatttgc tcgacggggt attcggtcat
    16021 acctttgggg agtttgtaac tccataattc ttccccttcc tcgttaaagg cgaacgcctc
    16081 aacttggcca aaatcctttg gatcgaggcc gcacaattca gatcgaccat cgccgtccaa
    16141 atcggcgcag acgagccagg caatggttcg ccccgggacg atgatttccc gcagacgctg
    16201 accatcgtgg gcaatttcca cggcgctgcc gcgggtcgta ttggtggcta agatgttgcg
    16261 ttgtttcgcc gggttcggct ccatgattcc tacacggaac gccgccgagg ctgtcctatt
    16321 ggaccaaatc ctctttccat cgagggtaaa ggcgtggacg cccaccaagc cgagaaaacc
    16381 ggccaccgct tcgatcgtcc cgtcgccctg aagatcaacc aagtgaacat cggctacccc
    16441 atcgtgcggg ggactatcat cggccgggta gcggctgaag gcagcgaaat tcttgtcgaa
    16501 gaagtagact cgttgggatc ccagtgacca cgctacaaaa aatcgattac cctggccatc
    16561 cacgccggtc ctcagaaaat tgattttgtc gggtggaatg gggttcaagg ggtgctgcac
    16621 cttgacctgg ccttcccaag tcaattctgt ggccatttgg ccgtcttcca cgacgtaaat
    16681 cgcgctctct tgatcggacg tctccacaat catcacgttt cccggcgatt tcaccgcagt
    16741 gcattggaat aacggtgtta accgcagatg ctgcggttgg gtcgcttcgg ctgcttgggc
    16801 tgttcccgcg gcagtgatct cttccacggg aaatgtaaac aatccgtgct tctcagactc
    16861 agccaccagc cgtgcgagct cctccctcat cttctggaag ccggattttt cttcttcggc
    16921 aaggttgacg ccgctgagga gcttctcaag cgtcgctccc aaacgatcga ctaaatctgg
    16981 ggctgaaccg agatgatatg cctgaatgac accctccgca ttaagaatca ccaggcaggg
    17041 gatcgtagcg gcctcccgcc ggatgtacac atcgaaacac ttttccgcgt cgttttgcgg
    17101 atcgcggagc agagggatgg agacactcca tgattggagg agatttaaca actcctgatt
    17161 ggagaccgcg cgactgtcca aagtatcgat cggagggtca atactgacag cataaaatgc
    17221 tgcgcgctct ttgaattgct cataaaccgc ctgaatcatc ctcaagacaa tctgcgagta
    17281 tgggtggttc ttcgaccaaa actcaaggat cactggtttg ttggccaagc tctgcaacgt
    17341 gacagggttg ccatcgacgt ccacaaactg gaaatccttt gggcgcgtcc ctaagaaatc
    17401 atagccgtgg gggtgcagtt cctcagtgat ccgcgactgc ggaggtgcct gaaattgaaa
    17461 ggccgccggg tcgatatctg catcaaggac ggcatttgga aactccgcat aaatcgcaag
    17521 attctgcaat tgtcccccgt ccaaggaccg tcggatgctt tcggtgggaa aatcgactct
    17581 cactaggagc cgggtgctcc tgtcgatcca aaggatgata tccccgtcca accgcgccag
    17641 gcgaatccga tcgcacgtag aattgccaag ggtgccaggc gggatgactt cgggaggctt
    17701 agcccgatac aagaacgtct tgagtggatc gtcggctagc aacagcacca gcggcggggg
    17761 cactaacgaa tacacttggc cgggcccttg catgagggcg ttcgcgagaa cggtatccgt
    17821 aaacagggtt tttagggtga acttatcggg tgcattggtt ttgagaacct gaccaggtaa
    17881 gaagttgacg aatccccaaa ggtcttgccc atcgcagatc aatactcctt cgcccgcgat
    17941 caaccgaagt ttgttgggtc gttcaaacgc cgttaggtag ttggtgcggt tctcaaatgg
    18001 ctgaccgtta tagacgcctc gcatcacgat gcgtccctga tctccgtagc tccgggcctt
    18061 gccgtaggcc tccaccatct tttggaggat ttcctcggcc gtctcggtac tgagaggagg
    18121 agcggcttgc tgactaccga caccaccagt gggttgggat actgcccggt ccccagtggc
    18181 ttgctggaca ggctgtcctt tctccgtcgt tggcgagtca cccgccttaa ctgtacctaa
    18241 aggctccttt tggtccgccg cctgcgaggg tccagctgac tctctacttt tcgccggaca
    18301 tccgcagaga gtcaacaaga aagcaataaa aacgagatga aaaacggacc gcgtggagac
    18361 gctgcgaaat atcgagagca gccgaaaggg atcagaggca caaactgtgc ataaaaacac
    18421 aacaaacgag ggaattcgtt cattttgcct catagataac tccgtttgcc aatccttggt
    18481 ctcgacgcca ccttgtactt gctcaggtcg tttcccgcct tgcccaggga agaacgcgat
    18541 caacgatcgc cgttaccagg ccgcactgga aataacatcg cgcgacccga gcaagcgcgc
    18601 ctacgcccat cttagcaaat ccttcccgtg tgtgacaccc aactgggaat aacagtgcgg
    18661 atgagggaaa atagagaacg taacgatcga atcccgcata ttctggacga acctatggat
    18721 atccgtattt cctacggcac atacggggtg cttgaaatta aaagcacctc gctggtaaac
    18781 gtggtcggct ttccgggatc tgatgttgca cccattgagg atgtgaacgc ggccgtccgg
    18841 gaagccttgg accatccttt agagttgccc gcattggcac gatgcgtaac accgggtgat
    18901 cgggtggtgc tggcgcttcg ccgcggaaca ccgcgtgggg cggaaatttt ggcggcggtt
    18961 actagatact tgcttcccca tttgtcaacg gagggacaac tcacggtttt gcgaactgag
    19021 tccgacgaaa cggcgggatg gggcgacccc cggccgtttt tgtcagcgga ggaagcacaa
    19081 gccttagagg tgcggactca tcgtgccgac cggcaggaag aacttacctt gcttgccaga
    19141 tttcgcaacg gacggccact ccggctgaat cgtgcgttga ttgatgccga tgtggtgatc
    19201 ccggttggtt gtactccacc agggaaggcc tggagtgctt acggcacttc gggggccgtc
    19261 tttccagcgt tttcggaccg catgagccag cttgcccact ggcgagatgt tgtgcaaaag
    19321 gcacgacgag gcaaggggcc attgcctgag catcgggatc gatctggccg agaggccaat
    19381 tggcttttgg gaacacagtt tgctgtcgag gtcatccctg gaccgtgcgg aagtgtcttg
    19441 agaattgtgg cgggagcaac ggaggcagca agacggaccg cccgtgagga atacagaaga
    19501 tggtggtgtc ccacagttga tggcaaggcg gagttggtca ttgcgggtgt ggatcataat
    19561 ccctcacaca caccctggga aaacctggct caggccgctt ggacagccgc ccggcttgtg
    19621 cgccgcggtg ggcaaattat tcttgtgacc gattgggacc aaggtacgat tccgcggttg
    19681 atcgacgaga acagcttggc cgaggtctct ggcatttgga gttggagtga ttttctccag
    19741 acccatggtg atttggctgt gcccttttgg cttcttgccc gggtacgacg ctatgcccgg
    19801 gtgtgtctct ggacagccca atccctcgcg gatgagtcac ttagcgggtg gcgggagcga
    19861 ttgtcgctgg agactacctc gggcgaggct gtgatcaatc ggttagtcaa tcgagttgac
    19921 tcgtgcacgg ttttttctca tgcgtcgttg gcatggccaa gagtgtctcg gggcgcaacc
    19981 ttaagaggtg caggctgata cgagaatggg caaaggcacg gagaaccgcg cggcattttc
    20041 cgatctttac tgcattaacc ggtttgtgat tgcggactgc gagatgtcag agagtatcga
    20101 agatattctt gaaagaaatc ttaatgctgt gcgacatcgg atagctgtcg ctgcgacacg
    20161 gtgtggccgt tctccgcaag agatcaccat ggtggcagta accaagtacg ttgatctgtc
    20221 aattatccag ggcctgctgc gtctcggcca acgaatcctg ggagaaagtc gtccccagca
    20281 gttgtggcag cgagcgagtg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 ntattgtgtt ggactgtcac aacgcggtag caaatcaagg aaagtgaaga gttcgaggat
    20461 ggaagcgaaa aatctggaaa ccgtacccga agctgttgtc ttcgacatgg atggcctaat
    20521 gttcaacacg gaggatatct actttgcggt gggagaggaa ctccttcgcc gtcgtggcaa
    20581 aaactttacc cgtgaattaa gtgatgccat gatgggccgc ccgccacaag caagcttcga
    20641 gatcatgatt cagtggcata atctttcgga tacttggcag gagctggcgg cggagtcaga
    20701 ggacctcttc attgaacgca tccaagggcg gattgcccca atgccgggac tgctggagct
    20761 gttaacagcg ttggaatcgg ctaacattcc gaaggccatt gccaccagta gttcacgacg
    20821 gacgcttcgc gctgtacttg cctgttttga cttggaaccc cgatttgctt ttaccctcac
    20881 ggccgaggat gttgttcagg gaaagcctta cccggaaatc tacctaaagg cctgcgaacg
    20941 atttggattg ccacctcaca aggtggttgt gctcgaagat agcgaagctg gttgccgagc
    21001 ggcggcggct gccggtacaa taaccgtcgc tgttcccggt agccacagcg cacgacatga
    21061 ttttacgtgt gcgacgatgg tgatcagcac tttgcgggat ccaagattgt atcagttgtt
    21121 gaggctgccc cgagagcgaa acagctaggg ctcactcgct acgcagcgtg gtcatttcag
    21181 aacagacgtc ggagaaaaag catgggtttt cattggtcaa gtcgcgtgct cggaagtctt
    21241 gtggttttct ccctttttat tgttgctggt tcgtcgttat ggggtgaaac aggtgacgaa
    21301 ctatggacgg cggcggaaat gcggattgaa aaataccgca aaggcgatgt cataatcacg
    21361 gttcgggaca aggatggccg tcccgttcca aatgccaagg taaggttaca gcagacacgg
    21421 cattgctttt tgtttggttg caatatcttt ttgtggggcc ggatcgaaga ctcacaactt
    21481 cagaccgcct ataacgaacg ttttgccgag ttgttcaatt atgcgaccgc ggcattctat
    21541 tgggcttcct acgaacggca gcgcggtcgt ccagatcatg attacacccg ccggctggcc
    21601 gaatggtgtc gggaaaaagg tattgtcgtg aagggacacc cgttggcttg gaatacggcg
    21661 gatcctattt ggctcccgga tgatccggat gaaatcttgc gtctccagct tgcccggatt
    21721 gaagactgcg tctcacgttt ccgcggtctt attgaccgtt gggacgtcgt gaatgaagtg
    21781 tcggagtatg atcgtcccga gtttgtcacg cggcgcgccc cgaaaatgtc ggccatgtgg
    21841 aagaaagtgg ggcagatgga gttcacgcgg cagtgctttg agaccgcacg caaggcaaat
    21901 cccgacgcga cgcttatcat caacgattat gtgacaagcc ccaagtttga gcgggtcatc
    21961 gagcaactcg tcgatcagga cggcaagcga ttgtacgacg taatcggcat ccaaagccat
    22021 atgcatggtg gcacttggcc taacgaacga atctgggaga tttgtgagag gttctcgcgg
    22081 tttcaagtgc ctatccattt cacggagacg actattcttt caggccagcg gggatgggaa
    22141 cgtccccgac cttgggtgac aacacctgaa ggtgaggaat atcaggccaa ggaagtggtg
    22201 cggttttaca cgatgctgtt ttcgcaccca gccgtggagg ccatcacctg gtgggatttt
    22261 tcagatttgc gagcatggca aggagcgcca gctggtcttg ttcgccggga tatgtcgcca
    22321 aagcccgctt acaccgagct ttacaagttg atcaagcaga agtggtggag tgatgaaacc
    22381 cttagcacgg acagccaggg gcgggcaacg actcgtgcct tcgcagggga tttcgttatt
    22441 tccgctcagg cccccggatt ctccgaggcc aaggaaagcg tcaaagtcga gtggcgaaaa
    22501 ccaaacgagt tcgttgtcgt tgtggagaag gaaggctgaa gtaatggatt tctgaggccg
    22561 agtgttgcga cgaaagacgc tgggcctgct ggtaagccaa gtgtggtaaa ttgtcgaggg
    22621 agaataactt ccttttgagg ggaaagcgcc ttgttttact ttaagccgtt atttccggct
    22681 taaggcgagc ggctgcgcat agacggagca gattgttgag ttcgctgcgg atttgttcgt
    22741 agggggcctc gtggcggatg gactgctcta gggcagtcgc ggcctccgta aggggagtga
    22801 agccgtagct tccggcagcg ccctttagtt gatggacagc ccgtcgcagt tcttcgaaat
    22861 cgcccgtgcg aaagagttct tcataaagcg ctgccctata tggcatctcg tcaacaaaca
    22921 tttggatgag gtcccgaaga tcagggtcgt ctgctaactc agaatatatg gcgggaacct
    22981 ggtcaagtgc tggattcgtc aattgttcgt tcatggatcg acctttcgcc gtgctaccac
    23041 atcgctaata gtgtcaaaac ccctccttcc caagcgggac acagatagtt ggtgtaccct
    23101 accccgcttg cttgatccct taagatagtc tagcttataa tgatgacgtg gttgggaaat
    23161 ctttcccagc tttgttgcgc cgaatcgtag gatcgattcg cccagtcggt ccgagagtaa
    23221 gcaccgcggt cagtcccgcc ataattcgcc ccttggattt gttagtcgcg agagatttca
    23281 cctgagagta gaacaaagga aaggagactg cttccttcgt taaaatctgt ctatcctgca
    23341 cagatctcca ttttttctgg ccaattgaat gggcgaacgg cgacaattag gtaaaatggg
    23401 gctttgcaat ggacgatgaa aaatagtggc cagaaggtgt ttgggtaaga aaattgggtt
    23461 aggtgttgtt tcagttcctc gagtgaacgg agagtgttaa catgaagcgc gtgacactgc
    23521 ttggcttggt ggcccttggg gccctggtga ctgtgctcgg cctgaatacg gctcgggctg
    23581 atttgttcct cggcggcgca tgtgcccccg cttgcgagaa gcccgcttgc gaacagcctg
    23641 catgcgaacc ggcttgcgaa cccgcatgtg agccagcttg ccgtcctggt ctcttggccc
    23701 ggattcgtgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caccaattcc
    23821 actccgaagg ccttgaggag ccgccgcctt tccagactca tactctccgg catcgtgacc
    23881 atcagcttgt aaccgcggga agcgcacaca tacgccaaac caattcccgt gtttccactg
    23941 gtgggctcga tgatgaccgt gtccggtttg atcaatccct ggcgctcggc ggcgtcaatc
    24001 atcgctcggg caatgcggtc cttgacgctc cagagggggt taaaactttc gagttttgcc
    24061 aaaagagtgg ccccatagcc ggcggcaagc cttctaactc taacgagcgg tgtgttcccg
    24121 atgcattggg tgatctcatc gtaacaacgg acttgggatg gaattgacat gtttgtgact
    24181 cctgattacc gacgctggct ttcgacttcc ccacaagggg tcgaaactga agctatagct
    24241 atacaaacct tttatgataa tcataatgat gattcggctt ttttcaagcc gatgcttaca
    24301 cgaagtcaac agcgccttct caaccccacc ttaaaatggc ccatttttcc ggttcctcaa
    24361 ttgggtttga caagcgcacg tccatctgcg tcgggcaatt ctacccccaa aagggtggca
    24421 atcgtgggag cgatgtcaac gatttggacc ttccctaaac gggtgcccgg cttgattcct
    24481 gggccccaaa tgacacacat ggcccacatg tcgggctggt cgggcaagta gccgtgtgtt
    24541 cccccaagac ccgaccgagt tgtcacaacc tcgtcgccag tggtagtctc gctaaaagag
    24601 taaccttttt ccgccgccag ccagatgtct ggacaccgag gatcctcctg gcctgttttt
    24661 tgtcccagag cttcgacttc tttgccggta agaaccgcct gaatcccttc cacagattgg
    24721 aacgcttgtc gaagctgttc catgagctgg tcccgctgtt gggtatcgaa aatgtagatg
    24781 ccggcggctc ccccttgcga catacaccag gccttttcgg gcactggctt gccatcgttg
    24841 acggagatta atccagcttg ccgcaaaagg acgttgggtt gaataacttt ttcgaatggg
    24901 aaaaaaccgt gatccgaaac gattaccaga tacgtgcttt ttgcccgcgg cgaagacgcg
    24961 atagcctcca ccaagtcgcg gatgcgatcg tcgctgtaac tggcacacca atatgcgtca
    25021 tcggagcggg gcccagtgcg gtgctggaca tggtccggtt cgacaaggtg aatcatgatg
    25081 agatttggct ggtgctgtaa cagaagctgg cgtgccatcc gcacgtacaa ccaatcgcgg
    25141 ggaacaccgc caccggcctc gcgacaccac cgcccatgac tatcgaccgg caagcctgcg
    25201 gcctttagtt cttgaatcca ggccggtgta ccgaagtcgg accacgccgt gtcgccgggc
    25261 atgtccggag cgctgaaatg cagtgttcgg gcatttcgcg tgacgggcca gagcacgccg
    25321 gcggtcacca atcccgcagc gtgggcgacg tcgtaaatgg tgggtgtttg caagatttgg
    25381 tctttgtcga agaccgggtc gcaaagcagg gttacctttt gcttcgtgtt ccggtcgatg
    25441 tagtcgttgg ccagaacccc atgacggtta ggccaaaccc cggtggaaag cgtggcgtgg
    25501 gcacaccacg tgaccgaggg gaacactgtg accatcgcgt cggctcgtgc tccttcnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga tcattggggc gacgaaatca
    25681 tccaccgtgg tcatccctgt cttcagaagg agatccaatg tccgcaggaa acggttgtcc
    25741 ccaggtaaga gcccgtaatg acgacacgtc acatagacgc tgatctgctc ttccgggtat
    25801 tcctcttttc tgacatggta gtcttgcgtc cgcgtttcga tattgacgcg gcattgcagg
    25861 ttacagtctt gatcgagggc gagggtcaac aatggttcgt aatggacaac tcgcgcggac
    25921 ggtacttcga ggagcctctc aaaggcaggt ggtacgccga ggacatcggc aacaagctga
    25981 ttgtgattac cctgataggt gtagtcgaaa ccaacgagga catcaaccgc ttcgcaatcg
    26041 agaggactca gcgagaggtg aaacggggcg ttctctaaga tcatgcggtg gtattgaaac
    26101 gcggcatcga ccgagggcgg attgacatat ccggcggaca gccgccgcac ttctaccgat
    26161 agccatcggt acgagtcgtt atcccgatca tcttccagaa ccacctgttc cccgccatcc
    26221 cgcgtataga aattccccat cgtggggtac gctttctgaa tgcgctcaaa gaaatggatc
    26281 aacgtttccc tgtgggcagg tagttcgagc acggtgttaa cggtcaaatt gatatagtgg
    26341 tcgtcacaca gatggcaata ggtatccatg attgtgctgg taatttgacc tgtctttacc
    26401 cacatttcta gcagcggggc aatgtctcgc cgctattctc caatccccat tttccataat
    26461 aatgagttcg aatataaacg gcactccgcc cagtgtcaaa acccccagga tttcctgggt
    26521 gcctacacac ggagggtgga aactaccctg tgtttattac gtaactagaa ccttggtgag
    26581 ttgccgcgcg tggaacacca gtcggtgcaa ccccttagcc tgtgcatttg gacaaggacg
    26641 cctgtccatt ccatagggtt cgttatatgg aatgaccggg tgctttgcgg ccatttgggc
    26701 gagcaggatg aatcaaccct ggtccgcaaa cttcgagaga ggttggggaa atgggcaact
    26761 tggaacaagg gagccaggag gcaactagac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn ngtgtcgtcg ggctccacag ggaccgcctt ttgcaggatg acgggtccat
    26941 tgttgtactg ctggtcgaga aagaacaccg tgcatccagt aactttgacg ccgtgttcga
    27001 ggaccgcagc gtggaccctt cggccgaagt agccctgtcc gcaaaacgct ggtgccaaag
    27061 ccggatgaga acctatcacg cggttttggt actccggcgt aatcgtaagg agcatcgcaa
    27121 accccgccat aatgacatac tcggccccca cctcttgaca acggcggagg atggcggcgc
    27181 tgtattcctc cgcggaattg aaatcgcgtc gctcaataac ctccgccgga atacaccgaa
    27241 attcagcgtg ctggagaccg ctcgcattag gggtactggc aatgaccagg cggacttcga
    27301 tggggaccct tccctcgtca acggctttca gcaagtcacg aagcgttcgg ccgctaccgg
    27361 atatcaaaat ggcgacaggt aggattctct tgtgcatagt tagtgacctt taaatggttc
    27421 tcttatcttc ggctggcacg ggaattatcc cacttctagg ggtgaaacgg tcattactgg
    27481 tcaattgtaa cgtgaccgaa attgggataa catgtctcag ccctctacgg aaatcctaag
    27541 ggcttattac caccattagg tggttgctcg cgaaaaatgt tgtccgattt tggcagagtc
    27601 agcgggatat tgtattaggg agaggcacga gaaaacgttc tgattctcta gtgcggggtg
    27661 aagagattct cgaagaggac tcgaaagcac gtgcgggagc aacagggtga acttccaccg
    27721 gatgaagagt ttgtacgctt gctttcaggc gttcagcgaa agctcttttt gttcgtgttt
    27781 acactggttc ccaatgtggt ggaagcagaa gagattgttc aaagcacaag cgtggtgctg
    27841 tggcaaaagt ttagtcagtt tcgcaaaggg gaggattttc tgcgatgggc ctgtggtgtg
    27901 gcacggaatg aagtattgaa gcatcgtgcc cgtgcaagtc gtcatgaggc cacattttcg
    27961 aatgagttca tggaacaact tgccgaaaca acggtgcctt taatggaggc tgtggatgaa
    28021 cggcgtcagg ccctgcaacg atgtctagag gagttgcagc cgcatcatcg ccggttattg
    28081 gcgcaacggt atcaagaggg gatttcaact gaagaaatag cgaaaaacca gggaaaaacc
    28141 gcggatgccg tgcggcggat gcttcatcgc attcgtttgg ctatttacga ctgtgtgcaa
    28201 aaaaagctgg cctctatgga gtgggggacc agtaccccat gaggtgacgg cgagcgatgt
    28261 cttcatttga tcgggaaaaa ctgacgctct tgatcgaccg cgtgctcgag gggtgcgcga
    28321 gccgggaaga gattcgcgca atcgaggagg gggctcgctc agcggatgcg caacgattcc
    28381 tggcagaata tcttttgctc gcgggcgagt tgcactggct tgctatcgat gacgatttga
    28441 aagaccgcga gatactggca cgcgtaagtc tgcctgaggg aacaagtgac cacggcgatc
    28501 gagacttgat tgcacgtacg gcggggtccg ggccatggtt gggcgggcaa ttccggaaaa
    28561 tggggctgtt ggtcattgcc gtgactgccg tgcttcttat tggtctcatc tcaatttggc
    28621 taagaaccgg ggcgagactg ccttcacaag ggcagttttc cacgccggtg attgcccagt
    28681 tggaaaaggt atatccggag gactctaaga agattggtgc cactattagg ccaggcgatt
    28741 gggtgatagt gcgggagggt gcagcccagt ggcaaatccg tggcggtgga agtctcatcg
    28801 cccaaggatc gacgcgtttt cgcctccttg aaaatgggca ggtggagctg gaatcgggga
    28861 cgattttttt gagaaccaaa gaaagtggac acttggccgg ggtaatcaca ccgcaggcac
    28921 ggtatttgga ccgcggtacc gcatttggcg tattgtgcga catgacgagg tcggaggtgc
    28981 acgtcctgga gggggaggtt gaggttatcg cgcgacggct cggcctggag gggtcacggc
    29041 gtgtggttgc aggtgaggcg ttggcctgtg acggccgtgg gcggacagag ccaattccat
    29101 gtcgcgccga gcggttttgt cagatggtgc ctgaggcgtt gacggtggcc gcgtacaggt
    29161 tgatggccct ttctgatcct cgactgtggc tgtatggggc gttcgaatcg ccgcgttttg
    29221 atggcaagtg gcacgcggtt ttgggaccga cagatttcca accggtctgg atgcgaggca
    29281 caatgtcgcg gctagacgtg cagcaaatca gcggctatga ctcgcagtcg agggccgtga
    29341 agctgattcg tggtacgtat tcgggcgata cggtgggggt ggggctccag acaatagatc
    29401 cagtcgtgtt accgagggcg atgaccgtgg agatgattgt ccggtttgac ggctggccat
    29461 cggcggggga aaataccctg gcatgcctct tggccacacg acaggatcag cgtcgatgtg
    29521 cagtgtggct aggcgtggtc ccggttgaga gagaaagcag gcacttgggg cggttagttc
    29581 acctctgcga cgggatggca ccatggacgg agacgaaggg gactctcgtc cccgggcact
    29641 ggtactatgt ggcggcaacc ttcgaacctg agagggacgc aacgcgggtg tccacgtggc
    29701 tggcagactt gacggaaaaa gagcccctgg tgtgtgtcct taaagatggg cagcttgcgg
    29761 ggcgtccagt ggacggttat ttagggattg gcaaaggatt cgacgatggg atgtcacatg
    29821 cgtacccatt tccgggtgcg attgacgagt tggccatcta caatggagtg ttggaggagg
    29881 atcttattcg ttttcatctg acacacttga ttgggacgaa ggggcaggag agtgttctcc
    29941 ctttaccggg agggccgtga agcggtggaa atgggaggtg tcatgacggc ggggtcggac
    30001 caggcaaaag gcgtgtgagt tggcagacga cgggtcggac agagaagacg agtcgttcat
    30061 tgatgagagg tgaaactatg ggtcgccatg gtggttatca gtctcggcgt gattttctga
    30121 gggtcacggg gttgagttgg gccattggtc aagcgggggg aatcgccgtg ccctcgctgc
    30181 tgcttggtga ggagaattcg ggcccgattg attgcggtcc gccgcccaag gctgccccgc
    30241 agcatcggac gggcggtgaa tcattcccgc cactgccttt gccggtaacg cctttgaggc
    30301 ggtcggagaa aaagcgtcct cccagcccgc cggcgcttat cggtaagatg gccttgggat
    30361 ctgtacgatg gatcactcgt gaggggaagc gggtgatgta ccgggactgg atgacagatc
    30421 ctgcagacgt caacacgcta ctcgagtgga cgaacgagaa gttggggatt cactatcggg
    30481 cggtcgagac cgattttgcc catttttcct ttgacccgcg ggaattgccg gcccttttgt
    30541 tcgcaggtca caatagtttc cagttgaacg atgaggtgcg ggcaaagttg gcccgatatg
    30601 tgatggacgg gggcacgatt atcggagatg cgtgctgcgg ctggaatgac ttcgccgaat
    30661 ccttccgcaa ggagatggaa ctaatctttc cgggaaggcc gttaagaaaa atgctgcccg
    30721 aggaaccggt cttctcgtca tattacaaac taggcaattt tacgtaccag aagggggacg
    30781 gctcgcaata tgacgacgca ccctgtctgg aatcgatcga ttttgggtgc cgttctggcg
    30841 tgatcttttc gcccgccgat atgacgtgtg gttgggatgg gcatgatcac ccgcggggcc
    30901 tgcgattgac aattgacctc gctcgtcagg ttggagccaa cttagtcacc tatttgctgg
    30961 gcacatacca attggcccgc tttttgagca cgaccaaggt ttactacgaa gcagaagccc
    31021 ccacgcggga cgacttcgtt tttgctcaga ttatccacga tggggactgg gaccccgatc
    31081 cgtcggcagt ccacaatttg ttgaagtttg caaaggataa ttccaccttg gaggtgaaat
    31141 tcaaacggca aaacgttcga ctgactgatc cccaggtggc caattatcct ctgctataca
    31201 tgacgggaca ccgtgagttc gtctggtcgg atgatgaggt ggccttcctc cagcgatatc
    31261 tcaaggctgg aggtctgttg ctggccgatg cctgttgcgg acgaatgtcg ttcgatatgg
    31321 gattccgccg ggaaatcaag aaagtctttc ccaatgctga gttggagcgc atcccgccag
    31381 atcacccgat ttatcacaac ttgttcgaca ttcaggaggt cgattacacg cctcgggtgc
    31441 gggaagattt tggccagctc aatgcgccgg agctcgaggg catcagtatt gatggccgat
    31501 tggcagtcgt ttacagccga tttgatcttg ggaatggttg ggagcagttc ccgcacccat
    31561 attcctacgg tctcaaagac gaggctgcac tgaagatcgg ctccaacgtc atcgtgtatg
    31621 ccattactca ctgataggcg gtcaaaatcc gacgaccgaa tccctagggt tggagaataa
    31681 ccttcattag cggttgccgg cgatagtaga gctggtcgaa ccaatacggc ccctcttcga
    31741 gcggggcgac ggcgctgatg aggggttcaa cccttacggc ctgcttggca agaagattga
    31801 ggcaccgggg ataatctcct tgagacgcgc acgagccaag tagcgtcaat tcccgggtta
    31861 cgaccttttg cagcggcagg gggacttctg gctggagatt tcccaccagg actacccgcc
    31921 cacctttggc aactgcgttg acagcgccgt tgaccgtctc gttaatgcct acggcgtcga
    31981 acgcaaggtc gacgggcggt ggtgctgtct ggcggaattg ctccggggaa agcaccaaat
    32041 ccgcagcgaa ttccttggcc atttgttggc gatgaggatc aggttcgatc gtgatgatct
    32101 gatcgagtcc cgcggaacga aggacttgga gaatgagtag cccgatgatt ccgccgccaa
    32161 aaatcacggc ggtttccccg attttgggag ccgcacgatc gacggcgtgc agtgctacgg
    32221 acaatggctc gatcattgcc gcttgctgaa aagagacatt ctcaggaaga cggtacacaa
    32281 tcctttcggg aacaacgaca tactctgcaa aggctccatc gcgacggtac tcgtcgcagg
    32341 agacaccgag gacacgccgg ttgtcgcata ggttgatttg cccgcggagg caataggcgc
    32401 attgaccgca atatacggtg gaatcgaaag ttacgcggtc acctgatcga aatcgcgtga
    32461 cctccggacc aacctcggca acgacgcccg ctgcctcgtg gcccatgatg agcggtggtt
    32521 tacgacgccc ggtgcttcca tcgagaccat gcacgtcact cccgcaaatc ccgcaggtct
    32581 gaacccgaac aaggagctcg cccggacccg gcttgggggt tgggacatct ctgacttcca
    32641 ggtggtgata ctcagtcagt acaagggctt tcacggcaaa tgtccttatc aaaagtctta
    32701 agtcagcgtg attggtactt atggctttgg cttcggcgac gcccataaca ttgggagatc
    32761 atgccgccgg agtgttgctt gtgtctctgt tgtttctggg ttccgatctg cgactacgtc
    32821 cttttctggt aactcacgta gcaaacaaac aagttcatcg ctaccgtgga gtggtgtaga
    32881 atcagagctt aggagccgat taactcctta ttgaaatgcg ccttagggaa tgtgagtcgt
    32941 gatcagggtt ctccgcatat tactaattac gacggccgtt gtgcttgccg tcgttctggt
    33001 tattgtgggg ggcatttggc tcctcgttac aagaacgcct gacttttatg agaaaaaagt
    33061 gcccgtcgat cccgctctag cgaaaaaagc gtgtgatcaa tttttgcaaa agaccagtct
    33121 cttcgccagt gatctgaagc gagaggggac gtggtacttg aaggtcacgg aggaggagat
    33181 caacggctgg ctggcggtgg accttccgcg taaccatacc caggccctgc cccgggggat
    33241 ctgggagccc agggttgaga tcggcgatga tatcattcgc gtggcggcac aggcggaata
    33301 cggacacctc cgtggtgtag cccacgtgga attgcagctg tacagcacag aggccaatac
    33361 tatcacggta gtggttaagc gggcggcatt aggggccttg cccctgcctc ttcaggattt
    33421 ggttgaacaa cttaagaaga tcgccgctcg agcccattgg gatgtccgtc agatgcaaac
    33481 ggggggatcg cccaccttgg ttatcacgat cccaaaaaag ttggacgaga agggccgcga
    33541 gctggaattg acgacaatac acataacccc tggcacgatc gaattgatcg gcaaaatcaa
    33601 gggaacccct cagcgttcct cggcggtatt ctgatgtgtg gtcgttgttg acacggcccg
    33661 atatgtcgag tcgattttgt ctagcaaggc tcggacctga ggatcgctgt cgcggccaat
    33721 cgcatcgcgg acaaatgaat ggatcgccgg gttagtggtg gttgccaaca ccgccagtgc
    33781 ctcccgtcgg acaattgggt ctgaatcttg ggccagtccg agaagaaaag ggagtggatc
    33841 cacgccggga aggttccaga gcaaatggat cgcctgacgg cggcgggcgc tgtcgggatg
    33901 aaatgccatt ctcccaacct caatggtagg ccgagttatt cccagggaga tcaatgtttg
    33961 cagggcacga tcggcttccg ccggactgcc agaaagttgt gcaaaaaggg gcgcgagcgt
    34021 cgcccaggta gcaccctttt ctgcggacgt ggtatcaggg cggttgctaa tggacctggg
    34081 aggctgatca cttggctttc ccgagctggg cctgctagat gactcatcga gggtcggtcc
    34141 ggccgatgtt tcattgcgat caggtgttat ctctcgagtg acggatttcc aaccaccgtc
    34201 ttgggtgcct tgtggggcga gatatgtggc catgctgccg gggccgaaag accgggaagt
    34261 tccttcagaa gtgctaactt ctttctcgta aatcgcaagt tgcgccagag gaccaccgaa
    34321 cacctctctc caggctgcca aatcgctcgt cgaaacttgc caggagtcaa caacggcagg
    34381 agaagagtgg tccgtgttca ttccttctgt tattggagaa acagcggaag ccgtcgcctc
    34441 cgcgttgggg cccgtttcgc gcaatgggag ggggtctcct gagatttgga gcattgttgg
    34501 aggggaattt ctcaggacgg tcgcaccgtt tgacgattca gccgtgtttg gaccgggcgc
    34561 tgccttgctc tcttctccaa cgggcctgtt atcaacaagt tttcgctcca acaaagattc
    34621 gcacagtttg acgatcttaa agccgttctt gcccccgtcc tcccatttga ggagttgatc
    34681 caacagtgct gccaggtgtt cttttcgtga cggggggata aagggcgaaa tacggtgcaa
    34741 ctcttcggcg atcaccagag cttcttgctc gccggttgct tgtgtctcgc gactgtgaag
    34801 tttacgccaa aggagtcggt atgcatggga agagatagtg tcatcgggat gagcgagtgc
    34861 tcgtaccaaa gcgggcgccc cattagctcc catttcggaa aactcctcta ggatcaggca
    34921 ggcttgatcg ggcggcactt gggcgagctg cccctcaagg tcgtgaataa gcctaatctc
    34981 ccaaagtccc cagagaatgg gcggtaaata tgcgaatgcc agcgagagac ccagaaggcc
    35041 aatcgctttg aacgcaatct ttgctagttt tcttaggtag cttttcacgt agcaaatacc
    35101 accttctatg ggttcggctg gcccctcgat gggcacgctc cccagttttt aaaaaacgga
    35161 catccatcac cgcgagaaag ccaatctttc ggatgacgaa tccgcacagg ttttgaaccg
    35221 gagattcggc ccccgatcac attgtgggcc gaattgtccc gcaattgccc gaatccgaca
    35281 acactaacgt tacctattta agtcggtcct gaggtggggg tgcttttttt caggttgcct
    35341 gccgggaatt ccgaataatg gttagagcct acaaaggaaa cctgtaatgc gcctctgtct
    35401 gttcgcgcag catcggcgaa ttgtcatcca ccggagttat tgaagactcg tgtccacatc
    35461 gaacgaccga ccaccagaaa acttccagcc ggtgagtctt tcccgggaaa aattttccag
    35521 ggtgtctttc ttcccgattt tatttattct tgcctgtctt tctgtgttcg ctatactgcc
    35581 caattttgcc agcggccagg gacagtatgg tgcttcacgg gggacctcgg aggcggtttc
    35641 ggtaagcgcc aaagaagaga aacagcaggc aatcaaccgc attcccctga cggagctcga
    35701 ccccgcctgg cggacgagag ttcttgatgt cgcagcccgc gcggctctgt tccgccgaat
    35761 gagttcgcaa atgatcgaat gcgaacctga gttctaccgc ttcctggtgg agcatccaga
    35821 agttgtcgtg gccatgtggc aggccttggg cgtaacgaag ctgaaactcc agatcgttgg
    35881 tccgcagaaa taccggctga acgatggggc aggcacgacc gcagtggtcg aatacctata
    35941 ctccagcccc aatcagcacg tcgtttattc cgagggcagt tacagtggcc cattgatgct
    36001 ccgtccagtg gaggggcgtg tccttgttgt gctaaccagc aattacctgc gtgaagctac
    36061 tgggacacct tacgtttccg cacaaatgga cctatttatt caggtcgatc gtgcgggaat
    36121 cgagctcatc accaaggccc tgcacccatt gcttggagga atggccgaaa ccaattacca
    36181 acaaacgatg gcgtttgtcg ggtctgtgta tcggacagcc gtagtgcgac cgcagtcctt
    36241 ggagcgactc gccagccaat tgacccaact ggaaggaaac acacaacaag acttcttgaa
    36301 ctggatcgcc cgcataggta acgattccgg caccacctac gaccgcgcgg cgatgattac
    36361 cccgccggac ggtatccacg aacgggcgct tcagccggaa tcgacaattc ggcaataact
    36421 gccggtttcc ctcgggcatc ttgcggcggc tttcctcagg cgattgcccg tctcacttgt
    36481 aacctaatct cggcccccgg caacctgctg cgccatttcc ttgagcgctt tgagatcgag
    36541 cttacctgac cccaacaccg ggatctgatc cactcggtag aagtgctcgg tgtcggggac
    36601 ccaaagcggg ggaagcccat tgctttgcaa ggcccggcat atttcagagg gggtgtgctg
    36661 caattgggtg taaagaacga caaggcgctc cccttttttc tcatcgggaa ctcccgtcac
    36721 agcaaaggtt atcgtctcct gatccccggt catctcgatc ctgccgatag cctcttccac
    36781 ggccagatgc ggaaccattt cgccggcgat ttttgaaaag cggcttaggc gtccggtgat
    36841 cgtgataaat ccatcctcat ccaattgggc aagatcaccc gtgatgtacc aaccatcctt
    36901 gatgaccttg gcggtttgct cagggtcgtt ccaatatccc ttcatgacgt ttgggccttt
    36961 gaccaagagc atcccctgtt tgccgacacc gagatcctcg cccgtttcca catccacaac
    37021 tttaacggca accccgggga tcgggcgtcc gacggttccc tgctttgccg agacaaacca
    37081 cggttcccga acacgggaag gcggcacatt cgaggagacc acgggagagg tctccgtcag
    37141 cccgtagcct tcccacggac gcacgccaaa cttttgctcg aaggcatcgg ccacttcagg
    37201 cggcagtttc tccgcccccg tgatcacaac ctcaagatgg gcgaaatcct ctggctcgca
    37261 acgtcgcacg taattcctta agaatgttgg tgtcgctacg aggatggtcg ccttatattt
    37321 gcggcaaatc tgtccaatgg gacggggttc caaggggctg aagtgataga ccaccatggg
    37381 cttaagttga agtgctgtcc acaaggtcgt cgtgtatcca aaagagtgga aaaatggcag
    37441 gataccgcac agaacgtcac tgcgacggag gtgcagaatc tggttaaacg ctgccacgtt
    37501 cgacccaatg ttgcgatggg tgagcatgac tcccttggga cgacccgtgg atccggaggt
    37561 gaaaatcagc gtgagtacgt catccgggtc gatacggtga agaccgagca gccgttccaa
    37621 cacccaggcc ggtaacagat acgcttggaa agcgctaata agcttatcag ccaactccag
    37681 ttgcgaatag atatcttcga gaaagaccac cgttgcctcg agcttaaagg ggaaacgttc
    37741 caacatccga cggctggtga gaacgtgctt aacccccgcg gagcggatgc aataattaat
    37801 caagtcctcg gaaagtgtgt agttgaggtt gacagcaatg cggcgatcga gggccagggc
    37861 agcattggtc acaacgccgg ccaccgagga gggtaaaagg accccaacct gtgtctcatc
    37921 tggtttcagg taatgacgtc gcaagaaacg tcgtagggcc agcgaggcaa tgagaagtcg
    37981 cccaccggtt agctcaatgc cggtagaatc ggcgatcttc cgtccccaca ataaggagcg
    38041 agccgtccgc aagaatcgcc gcggcgggat gtggtcaatc gtgttcttct gcgacaccac
    38101 ctcggctcct agcgacgcca gtgccagatg tgcttgccat gcttcccgga tattcctcag
    38161 cggttgcccc acataaagcg tgacccgtag tggtcgcaaa cccggccatt tccaaaagta
    38221 gcgtcccccg gaatatgtga agatacttcc ccagatgcca tcaatataca caggaataat
    38281 tgggacatcg agaccacggg cgattttcag aaagcctggt tggaagccac gtatttgtcc
    38341 cgtccgggtc atgccgccct cggggaagat gcccacaagc tctccgtctt ccaaggcttg
    38401 ccgggcctcg cggattccca aggccaccga ttttgccccg gggagaatgg caatcacgcc
    38461 agcccgcttt gctaagtcgc ggatgatcgg ggcattaatg tacttaaggt gggcaatcat
    38521 ccgaattggc cggggcatgg ccagcatgat catcatgcca tcgagccaac ttaagtggtt
    38581 ggaaacgata atggccgggc catctgccgg tacattttcg agccccacca cgcgaaatcg
    38641 atacagccat cgcataagcc aaccgaacaa gacccgaatc acatcgatgg gggtcaagta
    38701 caagatgatg agaaagacgg gaaacgtcac gatgcccgca caaacaaaca tttgtcgagc
    38761 gctaagaccc aggaaactcc ccaggaacca gtagatgacc gagcccatga tcatcccggt
    38821 aaagctcaaa aaattgtagg cagcaaggat gcggcctcgc tcgtgggcgg ggctttcgaa
    38881 ttgcaagatc gaaatcagcg ggatgtcgta caatcctgcg gcggctccga gaagaaagag
    38941 caggccgcac gctaggaagt atggggacga gttccagtga cccgtcccgg cgggaaggaa
    39001 tgcaaaggcg agagaaatca cagtgatgcc cagggcccct aaagggacga gtccccgttc
    39061 gatccgcccg tgagaccaca cgcccgcaag catactgccc aacccaatgc caagcgtcag
    39121 gatgcctagc atcggtccga cgtatgcctg gccatagggc cccacaagtt cgggtacggc
    39181 cagtttatcg atcgtaagct gcattaacag gcccagggtc caaaaatagg tacttcctat
    39241 ggcggctagg agcagcactc gatggcgaga taactccttg aggtcgtagt aacactgtag
    39301 gaagggattc aggggaatgc gtcgttgagg gtcagcggcg ggcagacgtt tgatcgcaag
    39361 gcttgtcagc catcctaagg tggccacgcc caagaggaca acggcattga tccaccagcg
    39421 atattgacct ggtccaagcc cacgcgcttg ggcaagtgcc ggatcgcgaa ttgtcgtgag
    39481 actgtataag agatttccca ccgacccgcc cacgatcaca gcgatcatcg tagtcagacc
    39541 gatgacaccg ttggcagccg agatgagttt cggtccgaca atttcgggaa tgctgccgta
    39601 tttcgaggga ctgaaaaaag cgctgtgtgt tccggccagg aagaggacta ccgccatggc
    39661 ccagacgtta ctggtcaaaa tggcgacgat ggccaaggtc atcacgataa tctcggcaac
    39721 tttcgcaccg atgatgacgg tccgtttgct gaaccgatca gaagtggccc cagcccagcc
    39781 tgccagcaga ataaagggca aaaggaaaat gaacgacccc accgatcggg caacgtcagg
    39841 gctttccttc aactgttcaa ggacgaggtc tttgccgatt gggataaaca accaccgcaa
    39901 aatgttgtcg ttgagagcaa cggtgaactg cgtcactaag agcgcgagaa aactacgtga
    39961 ccaaagactt gacgactgtt gcgatacaca ggctgcggtc gcgctagaat gtcggtcccc
    40021 cggcagtttg gtcatttacg gctccatagg atagcaaagt gaggacattc gggtttcatt
    40081 ttcgttctat gggaaagcat gcagtttagg gaactgtccc atttggatag cgtagtacct
    40141 gccctgagtt ttgacaattt gtcttgggca gattgcctct ttggacttaa acgcggacga
    40201 tagccgcaac aagtgtcatg agaaagagcg ggaagtggcg cactggggga tgggtccgcc
    40261 tactcccgaa agagtacccc aggttataca atgcgggttt tgggccggac acgggaatgt
    40321 tcgacctgtg gactatcgta gtttagggac cggaagcgcc aaaaactcat gagtcgcaaa
    40381 atcttagtta caagtgcttt gccgtatgcc aatggtccga tccacatcgg tcatttggtg
    40441 gaatacattc aaacagacat ttgggtgcgg tttcaaaagc tccgcggcaa tcgctgcatc
    40501 tacatttgcg ccgacgatac gcacgggacg gcaattatga tctcggcgcg tcgagaaggc
    40561 atcccggaag aagaatttat tcagcggatg tacgaggccc acgttcggga ttttgccggt
    40621 tttgacatcg aattcgacaa ttacggcagc acccacagcc cggaaaaccg cgagatttgt
    40681 gaggtctttt ggcgcgcctt gcgacaagcg gggcttgtca ttgaaaagga ggtgacccag
    40741 cttttcgacc cagtagccgg ggtcttttta gccgatagat ttgtcaaagg tacgtgtccc
    40801 aatccagcgt gtcatgcgcc cgaccggtac ggcgatagtt gcgatcgctg tggacacaca
    40861 catagcccag cggaattgat cgatccggtg agcacaattt ccggagccaa gccggaactg
    40921 cggacggccc gacacttgtt tgtgcagatc gaaaggcttc acgatttcct ggaagagtgg
    40981 acccaaacgg gtgatcacct acagcctgag attgccaatt atctcaaggc tcattttctc
    41041 agcgagccgc tccgggattg ggatgtgtcg cggccggcac cctactttgg gtttgaaatc
    41101 cccgatagtc cgggcaatta ctggtacgta tggttcgatg ctcccatcgg ttatatcgga
    41161 tcaacaaagc aatggtgtga tcgcactgga gagtcgcttg acgattggtg gcggaacccg
    41221 gaaaccgagg ttcatcactt cattgggaag gatattacgt attttcactg tctgttttgg
    41281 cccgcaatgc tcaagacggc cggtttcaat ctaccgcgaa agattcatat tcatggtttc
    41341 ctgacggtca atggcgagaa gatgtcgaaa tccaagggga cgtttatcaa agcggagacc
    41401 tatctgcgcc atctcgatcc gtcttatctc cggtactatt atgcggccaa gctcagctcg
    41461 cgggttgaag acatcgacct gaacctcgaa gagttcgtgt cgaaggtaaa cactgatctg
    41521 gtgggaaaca tcgtcaacct ggccagtcgg acggcccgct tcttagaagg gcagaatctc
    41581 tctcccacgt atcccgatga tagcggactg tttgcccagg ccgccgcaga cggccaggct
    41641 attgccgagg cctacgagaa gtgtgacttt ggaaaggcta tgcggcttat cctggccgcc
    41701 ggggatcgtg cgaaccaatt catcgaacga gcggcaccgt ggaacttgaa gaaagatccc
    41761 gccaagcaat cacaattgcg ggatgtctgt acaattggtc tcaatctctt tcggcaatta
    41821 gtcatttatc tgtccccagt gttaccacgg ctagctgcgc aaacgggtaa gctcctcaat
    41881 gatccggttt cttcctggga tcaatcgcag gtaccgttgg tgggcaatcg cgtggccccg
    41941 ttcgagcata tgatgacgcg agtgccaaag gaaaaactgg ccgccgtggt ggaagaaagc
    42001 cgcgaggtgg aagtcgcgtc tgggggcggc gaaggtaatc gcccggcgga gggtgtttcg
    42061 ccagagactg ccgacaccga tgagacgttg caaagggaac cgcttttgac ctccacggtc
    42121 acgatcgatg actttgccaa gattgacctg cgggttgccc gcgtccttgc ggctgaagag
    42181 gtacccggtg ccgacaagct gctgaagttg acactaagtc tgggtggcaa ccacacccgc
    42241 accgtcttcg ccgggattaa aaaagcatac aaacccggcg atttagtagg acgccttgtg
    42301 atcctcgttg ccaatcttgc gccacgtaaa atgcggttcg gaacgagcga agggatgatc
    42361 cttgccgccg gctgggggga cgaccaggta tttctccttg cccctgacca gggagcgaaa
    42421 ccgggtcacc gggtccattg aagcccatcc atttacgccc agttccaata gaaatcacgc
    42481 cagataaaca agggccctgg ctgcgcgcct acgacggatg ctcaggaaga ataccgcttt
    42541 tggatggcgg ccacctgggc ctgtcgggcc tgccacatgg cctcttctgc ttcggccagg
    42601 agacccgcct tctgacatat aagacttaag gcaacaaagc tgaatgggtc ttctggctcg
    42661 agttcgcaca ctttgcgggc attttctac
//
LOCUS       DAOSUT010000009        41410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_664_length_41410_cov_9.625632, whole genome shotgun sequence.
ACCESSION   DAOSUT010000009 DAOSUT010000000
VERSION     DAOSUT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 41410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41410
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_41410_cov_9.625632"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..1813
                     /locus_tag="PLD05_01585"
     CDS             554..1813
                     /locus_tag="PLD05_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76147.1"
                     /translation="MKRNQFSRRFFLQSLAATTVSVPWLVQGRALGLEDAAPASERIT
                     LGHIGVGGRGRDLLRGFMTCPGAQPLAVADAYRDRRDAMAEMIKGKAYGDFRELLARD
                     DIDAVVIATPDHWHVPIAVMAARAGKHAYVEKPLGLTVQQDLICRKVFQETNRVFQYG
                     TQQRSMAHCRFGCELVRSGKIGELKRIEVVAPDGGAGGSTEPAPVPPELDYEMWIGPA
                     PMKPYTVSRCVTPGTYWIYDYSIGFLAGWGAHPLDIMIWGCDADLAGPMTFEGTGEIP
                     KEGLYDTVIHWDVTVQMGNGIVMTFKPGSDSTKFIGTEGWVDVRRSGIDAEPKSLLKL
                     ELGPDDVHLTKSPRHDQNFIEAIKAGGKAIAPIEEAVRSDIISHMCDIAIRTGRKITW
                     DPQKEEIVGDAEASKMLSRPMRAPWTL"
     gene            complement(2037..5948)
                     /locus_tag="PLD05_01590"
     CDS             complement(2037..5948)
                     /locus_tag="PLD05_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76148.1"
                     /translation="MFTSDRIRLLLACLVAGGGLLGSIRATAEEEEVVGKRPYEMDWA
                     NRFEDTRPPLIDFEDLEGWTLEARDAVADFRRSREQQIWGKYVGKLTYRGVGPKPVVI
                     IRPPQAVAFRGPVDCVNVWIYGNNWAWVPDRTTPPVRVTVLLRSREGHPVRAEMGHVN
                     WKEWFLVHTRLHPRDVEALKDGGFVEGIEIAGGTNEEDRALYFDNLTPYLEPLPPLTF
                     QPRPRRPLTLPEGQPTGVHTGPGVLPFPTREETILPDQLTPNYQIEVLQNGEAYEFRY
                     RGDDGQLVYRYTPQHGHWGDITVTWNNALSFRPMVSGGIRLRTAEGRLVEPQYKLMSC
                     AVEGDAVVARWQVEAANCHGEVTYSFRLWQKSLVIDVACLGGIVGEVSFGYAEGLPHP
                     RLVTLPYLTCEAQRPAVVVAGDEKAPLFMMGLVDYYRSNASALFAINDSREDHLTYNG
                     GARYLPKTDGKLNDAFERLFLTVSPRFEEVLPNIPNPRSPWMHVTGERVWRAHGASNR
                     EKDYQLWKRVARYGMTKIVVTDHETGWRDGGESFTFRTRAAPGKGGDEGQRWYAEKMH
                     ELGFRYGIYNNYTDYAPVNEYWDEDCVTRLSDGNWRRAWPRCYNPKPTRAVEFEAKLA
                     PIIQEKFHLSTAYCDVHTAVRPWDYCDFDARVPGAGTFAATFYAYGEIMLHQKATWNG
                     PVYSEGNNHWYYCGLTDGNYAQDQVARLDINPWLVDFDLRKLHPLCCNFGMGNMGMFY
                     PRGPEGLDPETALDRFLAATLAFGHTGFLVMEGGMANAVRSYYSVQQIHARYAQATVE
                     DIRYRDAEGNMLPTAQALATGAFRRSQVYVKYANGLQLWVNGNMSETWDVPDVNMTLP
                     PNGWYARGSFPDGVLVAYSALVDGHRADYVDSPGYLYADGRGKVTRFPKAVCDGQIVL
                     LPQADGSFELIPLGCTVMGVFLDGRSAQAIALDEENRKELGPAEVRYSRAFVHVVPQE
                     GAFSYKLRPEPAPQVSLWCDRESVVPGETLVIFGPDAVKWTVPPDAAPRAHIWYQQDG
                     RWLDFRVVPLADLELTVKPQQWLLRARSNLPQEQEFSITLAEHTQKVLFPPGVWQDVL
                     FPFVPVEQEQVIDIPVEVSGGGCRLLKHWQLRCEKKRVEVVGFPSIQRTVQAFRQQEE
                     QPVSSATGAIVDRRTTRCGNVEKPCIFMHPPYQGGVGYVAALLEPVQLPPEPAAALRC
                     AVGKGDGSDPGDGILYRVYVLDAQGQTTLLGEKVQREHAWADWEVDLGPWRGQQIVLK
                     LVADVGNNNNSVGDWACWADLRIESREPQWVYTVLPVNPGR"
     gene            5947..6159
                     /locus_tag="PLD05_01595"
     CDS             5947..6159
                     /locus_tag="PLD05_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76149.1"
                     /translation="MALLPNAKANFIFAGMLLLVGRLAKAPPAKHNGFRPAPIMAMPL
                     GVGERLYTVKYEETLSCDIIGDEISA"
     gene            6126..7829
                     /locus_tag="PLD05_01600"
     CDS             6126..7829
                     /locus_tag="PLD05_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76150.1"
                     /translation="MRHHWRRNLSVMIIVIMSNLAVFFLGARTAIAQQASYPERWVWV
                     FGWGLGRDSDVTEVLNLLDRAGRSGYTGAAVSFGLDSLCKWDDARFRRLEVVKEACAR
                     NHLELIPSIFSVGYGGGILGHNRYLAEGLPVKDALFEVHGQEATLIPDPPVQIQNGGF
                     EEYSGQRFTGWRFHDQPGEVSFVDTQVKHSGQASLRMENFRANQHGHGRVMQEVKVHP
                     YRCYKMTLWVKTEDLQPTSAFMVQVLADNRALAPIGFNLSPTMDWTKLVLVFNSHRFE
                     VVRVYAGVWGGRSGKFWIDDWSIEEMGPINVIRRPGTPVSVKSEDGSVVYEEGRDYQR
                     LEDPNYSPYRVDRPAPPLRLLPGSRIKDGQRLRVSWYHAQKIHESQVTVCMAEPELYE
                     IFDHEAALLAKHVNPKKVILSMDEIRMGGTCEACRGRDMGQLLGECITKQVQILRKYM
                     PDLEVYIWSDMLDPNHNAHGDYYLVEGDFTGSWNHVPKDLVIAVWGGEPRPKSVQFFA
                     EQGFSQLIACYYDAPNLEGVKGWLNVVQGVPKVKGFMYTPWRKSYDLLEEFGRLVSGK
                     Q"
     gene            complement(8167..8751)
                     /locus_tag="PLD05_01605"
     CDS             complement(8167..8751)
                     /locus_tag="PLD05_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOP76151.1"
                     /translation="MVRTASTMMLPLGAPAPNFSLPDTEGRIVRLSDFRGAPALLVAF
                     MCNHCPYVKHVADHLAALAKEYQAKGVAVVGINSNDVENYPEDSPEKMREEVALRGYT
                     FPYLFDETQEVAKRYGAACTPDFFLFDKEQRLVYRGQLDDSRPNNGIPVTGKDLRAAL
                     DAVLAGQPVSPDQKPSLGCNIKWKPGNAPDYFGK"
     gene            complement(8937..9485)
                     /locus_tag="PLD05_01610"
     CDS             complement(8937..9485)
                     /locus_tag="PLD05_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76152.1"
                     /translation="MTEDLPTRESLTEKTSLPGYEQGSQREAKRFRAFLAGVMQGSMV
                     EKGLVGQDYRDQIKALFQQYLPEVDLYDPLADHRNSLEYDRRTGRDVFFYHNKMCQTI
                     DLLVAYLPQASMGTAIEMWEAYQHGAIVISISPMKHNWVVKFLSHRIYADLGAFAAAI
                     ASGEIRELLQEKRMRPPRSSEG"
     gene            9752..10810
                     /locus_tag="PLD05_01615"
     CDS             9752..10810
                     /locus_tag="PLD05_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76153.1"
                     /translation="MPRLPAAFGVILTAALCIGWAILRYPQVNQQVWEMVQAGRQPSA
                     DGSSGFHRLGLAIPANMAVAQAEQRSTANPTTVDRASPAATEHEDVEAGFAMVPITGY
                     VGANQAKPKDEKGPAQQSHLENVQVTLAVPPQMAILSQPNDDRFSGHDESETPDPSPG
                     QTESDGTGRDMMNPRAEVPSTPSLEEEPTERLPVPRSREESAPSVGETNVTAGDFTGP
                     GNSAQPTETVKVSSVSPSEMATDSGSHDDYVDPPLVPVIRNSSATGLAASLSETGGFE
                     VVAPHEAVARLAPSPPVTLPSDLPLVNIYRNRWFAAEKPQRLPPVEEASQDSDASAGM
                     ERYWDGAPSIPLYPSTGR"
     gene            complement(10818..11918)
                     /gene="hpnH"
                     /locus_tag="PLD05_01620"
     CDS             complement(10818..11918)
                     /gene="hpnH"
                     /locus_tag="PLD05_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HOP76154.1"
                     /translation="MQFPWSLIKAMTWHALKCRLTGRRRAPIVLMLEPLFACNLRCEG
                     CGRIREYHHVMDQRLSVEECLASAQECDAPIVSICGGEPLLYPELDRLVNGLIAQGRH
                     IYLCTNALLVDRWIDRLPRSSRLTWNIHLDGMEATHDAITQRPGTFQTVIQNIRRLKA
                     DGRRVTTNTTIYAQTDMDEILVLLDYLSELRIDGMLLSPAYPYPVVNGQPQRLPTING
                     QAQLPGRARELGKDHQVASQLFLTRCEVHAKFAAIRKRLEGYRLLNSPLYLDFLCGKR
                     EIPCAAWACPTRNICGWKSPCYLLTDTHYPTFAEWQEKTPWHQLGPAQDPRCHDCLVH
                     YGFETGAVVGGDLSLCEGLALVRWQLASPRAA"
     gene            complement(11948..14140)
                     /locus_tag="PLD05_01625"
     CDS             complement(11948..14140)
                     /locus_tag="PLD05_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP76155.1"
                     /translation="MEHSRHGCGAVKRSCLNDRAAQPAGDGVSLPSAIRRAVSLARWH
                     LLNRQHTEGYWQGELEGDSILQSETILILAFFGRENSPLVARLAERLLETQSEKGGWS
                     LYPGGPIDVSASVKAYFALKLAGYSPSDPRLRRAAEAILEHGGAQAVNSFTRFYLAFL
                     GQIPYHICPAVPPEVVLLPKWFPINLYKMSAWSRAMVVPLSVLWAMKPVRTLPPEKGI
                     RELFLTRPEDWPLTRGSGAHGGCDLWSLFFQMVDRCLKYAECWGFLPLRKRALRAAER
                     WMLEHMEASHGLAAIYPPMVWSLIALHALGYSQDSPVMREAWRRVEELLIDTEDQTSA
                     RVQPCLSPVWDTALTLRALMSAGLSTDDGQVQKAVAWLLDHQILTPGDWSHTVNVPPG
                     GWCFEHANPFYPDCDDTAAVLTVLATQCNHDEKQNSQLFPPQWRLVDEDRRKTPQTGQ
                     RSKSRGMSPQELEELAKRTNEAIYRGVTWLLGMQSRDGGWAAFDRDNCCAILTKVPFA
                     DHNAMIDPSTPDITGRVLETLGTLGYRVGFPAVDRAVAFLRRTQEPDGSWFGRWGVNY
                     IYGTWLAITGLRAVGVPEDDPAVVAGANWLLSHQLPDGGWGESPATYEHPHLRGDGPS
                     TPSQTAWAILGLLAAGKASDPALVRGIRYLLRHQLPDGSWPEEAFTGTGFPRVFYLRY
                     HMYPLYFPLLALARFATMNADRLAELDLPELHVVAPPIDVEDVTARIA"
     gene            complement(14325..15632)
                     /locus_tag="PLD05_01630"
     CDS             complement(14325..15632)
                     /locus_tag="PLD05_01630"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76156.1"
                     /translation="MKQPRGMMTRREMVKLSAVGLTVPTIIARTALGSEERLAPSERL
                     TLGFIGMGKQNQYHLNAFLGMKDVHVVAVCDVDTTRRNFSRDKVNAKYGNEDCAAYND
                     YRELIARKDIDAVVIATPDHWHTHPIVDACKAKKHIYCEKPLTLTIAEAKLCIEVVNK
                     YGVVFQTGSQQRSGREFRTACEAVRNGRIGKIKQVYASVGGPSKWCDLPEEPMEPGLD
                     WNLWLGQAPMRPYNSILSPRGMHDHFPAWRAYREYSGGGMTDWGAHHFDIAQWGLGMD
                     DSGPVEIIPPEQPEKGTGVRFIYENGVELIHGGYEGRGGVNFVGTDGVIYVDRGKLQS
                     WPEEVVKEPLKESEKVRLYESPGHHRDWLNAIRENRQPICPVEVGARSVTVCHLGNLA
                     YWTGKRLKWDPKNWRFVEPTEANEWLDGIRREPRRDPWQLPTI"
     gene            15994..17016
                     /locus_tag="PLD05_01635"
     CDS             15994..17016
                     /locus_tag="PLD05_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOP76157.1"
                     /translation="MERNPARRPSQSPQESERNEISSAEIMDYFLDKVEELHSSPAVI
                     WRVLQLLKDPEFDVRDVELLLEADPALSAAILRLVNSSAYGLPQKITSLRQAILLLGA
                     RSLRLAVLSFGLVDRLTKGTPAKVYEDFWRRALTTASAAARLARTYRTTNADEAYCAG
                     LLSELGVLALAQVDTRRYTRVYLTHRHGEDLCPAEQARYGFDHAALGERLLLRWGLPR
                     PLAQVAGSHHLVEGTPEALHAVVHAGSLLADVLWMPQSVLLPHARQWLSDRFQIDLDG
                     FITLAMDCKQDILDNAAMFSIHLEGKIDIDLLRREAQRQFKQVALETSLEYDAMTAVL
                     NQEMGI"
     gene            17322..17711
                     /locus_tag="PLD05_01640"
     CDS             17322..17711
                     /locus_tag="PLD05_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOP76158.1"
                     /translation="MIRFAWGKNGFWREERPSSQVVRLEELPAGERAQIVSLSGPEDV
                     VHRLEELGLRPGTVIRVFRPGNPCIVCLGGSKLGVRADGHVQILVSPLPGPPRCRHRH
                     GWGWRRAAIVSPTAPGKDVPDPLPPAN"
     gene            17841..20162
                     /gene="feoB"
                     /locus_tag="PLD05_01645"
     CDS             17841..20162
                     /gene="feoB"
                     /locus_tag="PLD05_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOP76159.1"
                     /translation="MAVSADKRCITVALVGNPNTGKSTLFSALVGVYQRVGNYPGVTV
                     EKRTGRFEFAGQSFEVTDLPGLYSLAPRSRDELVAVDVLLGREPGVEPIDIIVCVVDA
                     SNLERNLYLVSQLLELSVPTVIALNMMDVAERQGIQIDSDELARRLNVPVVPIQAHRG
                     LGVGRLRQVLAELADSPRTITPLTILPQAFGEACEQLLSELEKRDWLKCLSPQSSSHL
                     ARGEATFSQPNSPLKAPAYRFWAGRLLLDSTGFLQKAIWGERAKEVKRLVEAAREQLR
                     RQGFKVPQDETHFRYEWIRTILDGVVVMPAESSPTLTDRLDGVLTHRVWGTLIFVLVM
                     LLMFQAVFRLAAPLNEAIDSAIQGIAGWLTAEMAPGAFRSLLVDGVISGVGAVLTFIP
                     QIAILFLFVAVLEDCGYVARAAFLMDRFMSRIGLSGKSFIPMLSSFACAVPGIMATRT
                     IDNERDRLTTILVAPLMTCSARLPVYALLIAVFIPDQRYLGGLLGLQGLVLASLYALG
                     IVTAVVVAITLKTTLFRGRSPTFVMELPSYKWPSPRTIILRAWERVVVFVRNAGTIIA
                     AISILVWAALYYPHDLRSLPPELLRRQQELIVLRDTLPPGSSQRQRWETELEQIERQI
                     TAAYQRSSFMGRLGRAIEPVFRPLGWDWRIGTAVIASFPAREVVVATLGVMFNLDADV
                     AEANQGPSPFHDRLRSAVWEGTDRPLFNIPVALSLMVFYALCAQCAATLAVIGRETNS
                     WRWPIFTFAYMTTLAYLGALATYQIGARIMTGT"
     gene            21046..21888
                     /locus_tag="PLD05_01650"
     CDS             21046..21888
                     /locus_tag="PLD05_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76160.1"
                     /translation="MRVLFAISLVFAIVGCCASWTSGEEWVSGKIWEEPPIVTPGECG
                     SPPSDAIILFDGKDLSQWEGGENWKIENGYAIAQKTGITTKEQFGDCQLHLEFATPEV
                     IEGSGQGRGNSGVYLMGRYEVQILDSYENKTYFDGQCGAIYKQHPPLVNASRKPGEWQ
                     TYDIIFTGPRFDEAGNLLKPAYVTVIHNGVVVQNHFEIEGSTAWHVPPKYEPHGEKGP
                     IHLQFHGNPVRFRNIWIREIRELTYQQKGQEPEKKEPEKNEAEKAEAEKTEPEKKAEN
                     GQNQ"
     gene            22360..23373
                     /gene="argC"
                     /locus_tag="PLD05_01655"
     CDS             22360..23373
                     /gene="argC"
                     /locus_tag="PLD05_01655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOP76161.1"
                     /translation="MSIRVAILGATGYTAVELVKLLLRHPEVTITALTSRQEGNAPIA
                     SVHPSLVGRIQANLENLSAAEVAARAECVFSCLPHCASAEVIPELLKAGCRVVDFSAD
                     YRLASAQVYEDWYGHRHPDPDRLGKVPYGLPELFREAIPSADLVANPGCYPTSAILPL
                     APLLKEGLIAREDIIVDSKSGVSGAGRTPKLTTHFPECNESLSAYNIGRHRHTPEIEQ
                     ILSTVAGAEVQIVFTPHLVPMDRGILTTTYSHPTRQVTEDEVLDCLRSFYDDEPFVRV
                     VDHLPATKDTSGTNFCHITARIVRDRIITVSCLDNLIKGASGAAVQNFNLMYGFPETT
                     ALL"
     gene            23474..24667
                     /gene="argJ"
                     /locus_tag="PLD05_01660"
     CDS             23474..24667
                     /gene="argJ"
                     /locus_tag="PLD05_01660"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOP76162.1"
                     /translation="MAVRVPKGYLVAGVHCGLKRDLRKPDLSLIVSEVPATAAGVYTQ
                     NIVCAAPVQLDRRRTPSNRIRVVVINSGNANACTGERGLRDAEEMARLAAKAVGAEED
                     QVLVMSTGVIGVYLPMEKIAHGIELAAARLGNDEEALIMAARGMMTTDTVYKLSGRTL
                     SLARREIQITGMVKGAAMMGPNLATMLGVIFTDATLEVDTAQQLLREVVDDTFNCINV
                     DGHTSTNDTVLFLANGAAGGPPLSGDLLDDFKQAFHSLCTELARMIPADGEGATHLIT
                     IEVRGCRTRADALQIARAIANSPLVKAAIHGADPNWGRIVSAAGYANVPFDPNKMTLY
                     LNGTCLYRDGTPVKFDEIEVSNSIRAERETRIELIFQEGSASGRFWTTDLTPEYVRLN
                     AEYRT"
     gene            complement(24769..28158)
                     /locus_tag="PLD05_01665"
     CDS             complement(24769..28158)
                     /locus_tag="PLD05_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HOP76163.1"
                     /translation="MIPGRDGCASGAASAKTDGKFGIGKESPTLSTKDVAPIRIIWRW
                     VQRTVGVVPLGERCDRAAALAFVLSMTWNGLWLLAIFVTAFATGRVFAEDVSFPLPNR
                     SFPIDIKAAAGNHWRMGSYEVWILRGWCEIRQGSVSLRARESVIWIDRQAEPSVGLGD
                     PVTPGVAAPALEEGPLLLAPIPDETSDRSGSAFALPRHASGTGRLIKVIIYLEGRAQV
                     VSDDPQHRLQIQDKTWFGRLYTTADVNVQVKEEAGEPEPYPAIFARAMARRNAATEGL
                     TQSVLRPNEFLSDPSPRGASEGAMRRIRVFPRGDVPIQAQWFPAPQGGQWIGVIDAGI
                     NLIVEEPGPWGTLDVSADRVVIWYTGDEAPNLSGEATTPQQWPLELYLEGNIVFRQGD
                     RIIYADRMYYDVARRAGMILNAELLTRIPDYEGYLRLRADILQQMNETQFFAQNLFVT
                     SSRMGVPGYRLQTQFAYVEDRVQPVLDPTTRQVVIDPQTGQPAVIHRPWALARDNFIY
                     LRNIPIFYWPVLAGDLTKPTYYVNRIRLRHDSIFGTQVLTNWDAFQLFGIRNRPEGMD
                     WSLSLDYLSERGLGHGTSLTYSRDGFLGLPGYATGLADFWGIYDNGLDNLGRGRRALA
                     PEADYRYRLLWQHRQYLSEEYQLTGEVGLISDRNFLEQYYEREWDELKDQTTSLSLKR
                     IYGNASWDLSASVRLNPFFTQTDWLPRFDHYKLGESFFNDAFTWYAHTSIGLARFRTA
                     SAPSNPEDLAAFSYLPWETSSLTGDPLSTFSERMITRQEIDWPFQLGVIKIVPYALGE
                     LGHWGEDRSGDSLQRGYVQLGARANLPLWAVNPYIESELFNVHGIAHKINFKAEAFWA
                     SSNRDLDALPLYEPLDDDAIEAFRRRLGVLTFGPTNGFGGPPAPYAFDERSYAVRSGL
                     ASWVTSPSMEIADDLTLIRLGVEQRWQTKRGMPGSRRIVDWITFDTHFSLFPDSSRDD
                     FGRFVGLLDYDARWFVGDRLTLASTGLFDFFDSGQKLVTFGGMLSRPPRGDLYVGLRI
                     LQGPIDSTVLFLSYSYWMSPKWMSTLGVSIDLGESSNIGQSFSLTRIGESFLVSLGLN
                     VDGSRDSVGVHCSIEPRFLPKRRIDQVTGARIPIAGSGGLE"
     gene            28663..30018
                     /locus_tag="PLD05_01670"
     CDS             28663..30018
                     /locus_tag="PLD05_01670"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOP76164.1"
                     /translation="MDLKRVTLPEKELPSEWYNLLADLPQPLPPPVHPVTKEPLDPAA
                     LERIFARELIKQEVSQERWIGIPDEVRRIYAIWRPSPLVRARNLEKALKTPARIYFKD
                     ESHSPPGSHKPNTAVAQAYYNKIQGIRCLSTETGAGQWGSSLAFACSFFGLQCKVYMV
                     KVSYHQKPYRRVMMQLWGASVTPSPSEETESGRKILEADPNCPGSLGIAISEAVEVAI
                     KNDDTNYTLGSVLNHVMLHQTVIGLEAEKQMKEFGDWPDVVIGCVGGGSNFAGLAFPF
                     LRHKIAGQDIRFVAVEPKACPSLTRGMYRYDFGDTSMLTPLLKMHTLGHSFVPAGIHA
                     GGLRYHGMAPMVSFAAKLGLIEAIALHQKECFEAARLFAATEGIIPAPETSHAIRGAI
                     IEALKCKETGEEKCILFNLSGHGICDLGAYERYLAGELEDFELPQEQIERALAELPVV
                     E"
     gene            30268..31482
                     /locus_tag="PLD05_01675"
     CDS             30268..31482
                     /locus_tag="PLD05_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOP76165.1"
                     /translation="MGPRRRHTMPLGRMIACHLMGYLAAVLLWGSALAEKIGPWELET
                     LYQPPRAEWGEVKDGIREVYYEGEPYQGHATRVYAVYAQPEGEGPFPAMVCVHGGGGT
                     AFPEWVRLWASQGYAAISMDLAGCGPERKRLSDGGPDQTDVSKFRDFDEATVTDMWSY
                     HAVAAVIRGHSLLRSLPQIDDDRIGVTGISWGGYLTCIVAGVDHRFSLAVPVYGCGFL
                     HENSVWLPRFEKMAPEQRERWIRFFDPSNYLGRVKCPIFFLNGTNDFAYPLDSYRKSY
                     DRVPGEKLIRIEVRMKHSHPDGWAPQEIRLFADSLLKGGTPLPKLTPLRRDGRRVWAE
                     VTSQTALREAHLNYTLDAGRWQERQWVTVPARLEGQRIVAELPEPQPTAFYLDVVDER
                     GAMVSTPPEILR"
     gene            31677..32600
                     /locus_tag="PLD05_01680"
     CDS             31677..32600
                     /locus_tag="PLD05_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76166.1"
                     /translation="MIDFDAFSRHSRRDTLKGLVAGGAMILGIKGFGHMGFFAEEVTG
                     AAQALEKLGWTVACQLYTFRRFPFYEAIEKIAALGIKAVEPCFFLALDKGQPGLVTSE
                     ALTPEKRAEMKQRLARLGMTMPNFYANVGGDAEQAKKVFAFAKEMGCQTIVAEPPPEA
                     LPMLDELLAEYGLRLAIHNHPKSPQSRYWHPKNVLEAVRDRSERIGACCDTGHWVRSG
                     LDPVECLKMMEGRVITLHLKDVAEVGVVEARDVPLGTGAAKYDLVLQELARQKFRGVL
                     PIEYEHDSPQLEEDVAACRDFVLGWAAKNVS"
     gene            32661..34277
                     /locus_tag="PLD05_01685"
     CDS             32661..34277
                     /locus_tag="PLD05_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOP76167.1"
                     /translation="MACHRSPAPSVFKHWFIGFVIGVSCLLARGQRPFGAMEGFIVFA
                     AEDNPQFSKLWGREGELWTPQSRLPDFSYAGYRWGQESYRIPKESISVKDFGAKGDGQ
                     SDDTAAFKRALAAGKGKVIVIPPGRYILRDILTISSSNTVLRGAGPGKTFLVFTKPGI
                     EIDPRPSKTDGNQPTTNWSWAGGLISIGGRRQQPRDVVKVVGSQKRGDTQLSVEKNPF
                     RPGDEILLTLRDDEKNSLLTYLYRGQPGDISGLRNWSVEQVFRIVSVEGSTLKLNRPL
                     RFDIRSEWQPTVERFLPPLADVGVEGITFDFPVQQYAGHFKEIGFNPVEITRSAAHCW
                     LKDLVIRNADNGPFVSGYFCTVEKIRFEADPQRVSSSGNYTGHHGVTFLRCSDCLCRD
                     FEFNTRFIHDLTVQSSIGCVFCSGRGIDICFDHHRWAPYENLFTDIDAGTGKRLFASS
                     GGGMRGNHTAGGETFWCIRTQGLVRWPKSLGIDAINVVGLNVSDAAEAVPSLPSPTEL
                     TGRWCEPISPEVLQPQNLYDAMRKRVASPQ"
     gene            34778..35038
                     /locus_tag="PLD05_01690"
     CDS             34778..35038
                     /locus_tag="PLD05_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76168.1"
                     /translation="MTRLFSQRRRRPSRRGILSFEWILLISILVIGIIGGLAAVRNAL
                     LSELNDLSQAVEAINVCGTCDPASCPPGNPDCCDDPWWCCGP"
     gene            35220..35924
                     /locus_tag="PLD05_01695"
     CDS             35220..35924
                     /locus_tag="PLD05_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76169.1"
                     /translation="MLPRLAVGCFFLLIAFLFRAFWLPVVAYPLMAPAPQHENAVTLI
                     WARSSDGKTPSGDGIVERTASLLKTHPHARVIFTQGWSSRLVELGVIPPPYETFRSAL
                     AKYQIESERVINLEVNGFEFWNEAKAVDAFLREHPEERVLLLVEEFAGQSHRYVLRRA
                     LDVSHRDRVEILGLPDLQYSYHDWWRSRSGVKGVMVGYLFLIHTILQPQPLRPPRKLS
                     LEDFEQSIPEAPGGRP"
     gene            35921..36649
                     /locus_tag="PLD05_01700"
     CDS             35921..36649
                     /locus_tag="PLD05_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOP76170.1"
                     /translation="MKRVWWLLACLILSGLAILFHGRWLPSLAKYLDVGEAPRKSDYI
                     MIMGGGVENRPFTAAALFKKGWGERILIAELRPTPGGENLLPPEHEVTSRLLQKLGVP
                     EDRIIVLRGNHAATFHEIVTLGELLAREPEARVMIVTDPVHTRRTRWSVQRILGERAS
                     RISFVSAPSDGYNPDDWWQTGQGFFMVTSEYLKLLFYAFRYGWGLHLTAAAVALILLI
                     GILARYLRPMGRDKASTREAVPTS"
     gene            36937..37602
                     /locus_tag="PLD05_01705"
     CDS             36937..37602
                     /locus_tag="PLD05_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOP76171.1"
                     /translation="MTESSHTFSSEKKIRVFIADDHDMVRIGLKAMLSVPDIEVVGEA
                     GTGEDAVNFLLNNDVDVAILDVRMPRGDGLNALGRIKLEKPELPVLMLSTYDNPTYIA
                     RAVAFQAQGYLLKSCSREELLDAIRKAARGENIWTRDELRRVAGGLATPRMAADVDIP
                     LTQRESEVLKQLANGLTNKEIAQILNISYETVKEHVQHILRKIGVTHRTQAAVWAVKN
                     RLV"
     gene            37755..38690
                     /locus_tag="PLD05_01710"
     CDS             37755..38690
                     /locus_tag="PLD05_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOP76172.1"
                     /translation="MVQFPVSIVDLPKTGVLTDDPSPCPQMDSAACPQMRHRPAGNVQ
                     LPPGAETSQVGESPDPRLTLPAPVSLLGLPIVPWRHYEVIHVIDWLVGRGEPSYFVTA
                     NLHYARLSATIPALKAVNDHAAFIVADGMPLVWLSRWRRKPLPERITGADLIWSMCEL
                     AANRGYRVFLLGGAPGVAETAGQRLQARLRGLQIAGIAAPCLDTLTPLERTRLITQIR
                     QARPDILFAAFGQPKGELWLAEHIQELGVPVAVQIGAALDFVAGRVRRAPRFVQRMGL
                     EWAYRTLQEPRRLLPRYLADGWFFLCQLMRSSPRS"
     gene            complement(38735..40852)
                     /locus_tag="PLD05_01715"
     CDS             complement(38735..40852)
                     /locus_tag="PLD05_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOP76173.1"
                     /translation="MVRLISRSVFRENDAAICRRDRASIARCRLLGAVMILGGAILLS
                     PLEALCVEQAPFAMRRPVPDLPENLEWLNTTGPLTWRDLRGKFVLLDFWTYCCINCMH
                     IIPELKKLEAKYPRELVVVGVHSAKFQEEKESQNIVQAILRYGIEHPVINDSDMLVWR
                     RFGVNAWPTLVLIDPEGFVVWAQSGETTFEALDAILAPAVAFYDKRGLLDRSPVHFVT
                     ESSRAARTPLRFPGKVLADEAGERLFIADSGHHRIVVCRLDGQVVDVIGDGIPGLRDG
                     AWSEARFCSPQGMVILGDRLLVADTENHAIREIDFTKRTVRTVAGTGVQRRRPPVMRM
                     GPPLQAELSSPWDLCVVGDWIYIAMAGCHQIWRMDTALTRIGPYAGNAREDIVDGPLL
                     PPSAYALGFASFAQPSGLATDGEWLYVADSEGSSIRAVPLQPRGEVRTIVGTSRLLAA
                     RLFTFGDRDGPPDQVLLQHPIGVCYGNGFLYVADTYNNKIKRIDPRTGTTMTLVGDRQ
                     AGDTNDPPRFDEPAGITFARDRLFVADTNNHAIRVIELQPTLKVTTLALKGLEPPPAV
                     EEKGTFPEDKVDLRQEPVVRLRPGGTIKLQILFEFLAGFGLNPNVPTDLVIERLSDQG
                     VDSAGDKQVVRIPPGEGPLEVSLTGLNSNVKSLRIVCTYFWCEKSGRGLCRIGTVGWL
                     VPVQWADDGGDSIVLKHRVGGGS"
BASE COUNT         8653 a        11848 c        12165 g         8744 t
ORIGIN      
        1 aaaatcaaat aaataggatc aaagataaac atatttgcct caaaaacctc cctcggctgt
       61 caatcctgtt cggaaattcg acacacgtgt gtcttagcct gtccctcggg gaaagcccct
      121 ttttactgta ttctatgttt tcctgcgcgg ttggccagtc cgatttgagg tcgcacacgt
      181 cgcggtttac ttccacggtt ggacaatctg tagtagcgag gacgggctct gccgctgtcg
      241 gtagctggga ggcgccgttt gtcgcaatat gcggagggac gcgggtcgtc gtgtccgccg
      301 tggtgccaat ggatgatcca tggtcgtcgc cgactacggc agcgcgtcgc cttccattac
      361 ccccatctta cgagcaagag cgtggtggct tgcacgggca aatggggcgg tctaccataa
      421 ggtagccttc ctgaaggcct ccagatgtcc agtttgccga agggtctgtg aagcttcggc
      481 aacatcttgg gcgtaaagag cgccaccggt caagagcgtg tggatttact tgcacaatcg
      541 acgaggtacg gccatgaaac gaaaccagtt ctcgcggcgg tttttccttc agtcgttggc
      601 ggcaacaacg gtcagtgtgc cttggctcgt tcagggacgt gcccttgggt tggaggacgc
      661 ggctccggcc agtgagcgga tcacgctggg acatatcggg gtgggtggac gtggtcgcga
      721 ccttttgcgg gggtttatga cctgccctgg cgcacaaccc ctggccgtgg ccgacgccta
      781 ccgtgatcgg agggatgcca tggctgaaat gatcaagggc aaggcctacg gagatttcag
      841 ggagcttctt gcccgcgatg atatcgatgc cgtggtcatt gccacgcccg accactggca
      901 tgtgccgatt gccgtgatgg ctgcccgcgc gggaaaacac gcctatgtcg aaaagcccct
      961 cggtctcacc gtgcagcagg atttgatctg tcggaaggta ttccaggaaa cgaatcgcgt
     1021 ctttcagtac gggacgcagc aacggagcat ggcccactgt cggtttgggt gtgagcttgt
     1081 ccgtagcggc aagatcggcg agctgaagcg gatcgaggtc gtcgcacccg atggcggcgc
     1141 cggggggtcc accgagccgg cccctgtccc gccggagttg gactacgaaa tgtggatcgg
     1201 ccccgccccg atgaagccct acaccgtcag ccggtgtgtc acgccgggga catactggat
     1261 ttacgattac tcgatcggat tcctggcagg ttggggggcc catcctttgg acatcatgat
     1321 ctggggatgt gacgccgatt tggcggggcc catgaccttt gagggaacag gtgagatacc
     1381 caaggaaggt ctctacgata cggttatcca ctgggatgtt acggtgcaaa tgggaaacgg
     1441 catcgtcatg accttcaagc ccggtagcga ctcgacgaag ttcatcggca cggaaggctg
     1501 ggtggatgtc cggcgatcgg gaatcgatgc ggaacccaag tcgcttctca agttggaact
     1561 tggtcccgac gatgtccacc tgacgaagag tccccggcac gatcagaact tcatcgaggc
     1621 catcaaggcg ggcggtaagg cgatcgctcc cattgaggag gccgttcgtt cagacatcat
     1681 tagtcacatg tgcgatattg ctatccgcac tggccgaaag atcacctggg acccgcagaa
     1741 ggaagagatt gttggcgatg ccgaggcatc gaagatgctg tcccgtccga tgcgggcacc
     1801 gtggacactc taaatgaaac ctacgtgtta gttgcgccgg aactgggagg gacgcggtcc
     1861 cccgcgtccc ttgtcttttt ggagggactc actccgtggc gtgcgccctg gttcgccggc
     1921 ggaaggtttg gattatcttc ggtcacgaca cagcgcggcg ctgtatcatc gccacccgga
     1981 ggcaacgcgg tcaccaccgt ccgggcgtgt gtctgcggct cgtgttagct gaacggtcaa
     2041 cggccagggt taacgggcaa gacggtgtaa acccattgtg gttcacggct ttcgatccgt
     2101 aggtctgccc agcaggccca atcgcccaca gagttgttat tgtttcccac atcggccaca
     2161 agcttcaata caatctgttg acccctccag ggaccgagat cgacttccca atctgcccag
     2221 gcgtgctcgc gttggacttt ttcgccgagc agggtggtct gcccttgggc atcaaggacg
     2281 taaactcgat agaggattcc atctcccgga tcactgccat cgccttttcc gacagcgcag
     2341 cggagggcag cagcgggttc tgggggcagt tggaccggtt ccagaagggc cgccacgtac
     2401 cccacaccgc cttgatacgg cgggtgcatg aagatgcacg gtttttccac attgccacag
     2461 cgggtggtcc gtcgatcgac aatcgccccc gtggcactgc tcaccggttg ttcttcctgc
     2521 tgacgaaaag cctgcaccgt tcgttggatg gaggggaagc caacgacctc cacgcgtttc
     2581 ttttcgcagc gaagttgcca atgcttgagg aggcgacatc cgcccccgct cacctccacg
     2641 gggatgtcga tcacctgctc ctgttccacc gggacaaagg gaaaaagaac gtcctgccac
     2701 acgccagggg gaaataatac cttttgggta tgctcggcca aggtgataga gaactcttgc
     2761 tcttgaggca ggtttgatcg tgcccgcaat agccactgct ggggcttcac cgtcagctcc
     2821 agatcagcca aggggaccac gcggaaatcc agccatcggc cgtcttgttg gtaccaaatg
     2881 tgggcacggg gcgcagcatc tggtggcaca gtccatttca cggcatccgg cccgaagatg
     2941 accaaagtct caccaggcac tacagattct cggtcgcacc aaagcgatac ttggggtgcc
     3001 ggttccggtc ggagtttgta actgaaggcc ccttcctgcg gtaccacatg aacaaatgcc
     3061 cgactatacc ggacctcggc cggtcccaat tctttgcgat tttcttcatc gagggcgatg
     3121 gcttgggccg agcgtccgtc tagaaaaacg cccatgacgg tacaccccaa agggatcagt
     3181 tcaaaggaac catctgcctg gggcaataag acaatttgcc catcgcaaac ggccttagga
     3241 aatcgggtaa cttttccacg cccgtcggcg taaagatacc ctggcgaatc cacgtagtcc
     3301 gcacggtgac catcgaccag ggctgagtac gccactaaga caccatcagg aaagctgccg
     3361 cgagcgtacc agccattggg cggtaacgtc atgttgacat cggggacatc ccaagtctcg
     3421 gacatgtttc cattgaccca aagctgaagg ccattcgcgt atttgacgta cacttgcgat
     3481 ctacggaatg cgcctgtagc tagggcctga gcggtgggga gcatatttcc ctctgcgtct
     3541 cgataacgga tgtcctccac cgtcgcttgg gcataccggg catggatttg ctgaacggaa
     3601 taatagctgc gcacggcgtt ggccatcccg ccttccatga cgagaaagcc tgtatggccg
     3661 aaggccaagg tggctgccag gaagcgatcg agtgccgtct cggggtcaag tccttccggt
     3721 ccgcggggat aaaacatccc catgttgccc atgccaaagt tacaacacag ggggtgcagt
     3781 tttcgcaaat caaaatcgac cagccagggg ttgatgtcca gtcgggctac ctgatcctgg
     3841 gcgtagtttc catcagtcag accgcaataa taccagtggt tattgccctc actatagacc
     3901 ggaccgttcc aagtggcctt ctggtgaagc atgatttcac cgtaggcgta aaatgtcgcg
     3961 gcgaacgtac cggcaccggg cacacgggca tcaaaatcgc agtaatccca tggccgcacg
     4021 gccgtgtgga catcgcagta cgcggtgctg aggtggaatt tttcttgaat gatgggagcc
     4081 agtttggcct caaattcgac cgcgcgggtg ggttttggat tgtaacaccg cggccacgcc
     4141 cggcgccaat tcccgtcgga aagccttgtt acacagtcct catcccagta ttcattgacg
     4201 ggggcataat cggtgtagtt attgtagatg ccgtaacgga atccgagttc gtgcatcttt
     4261 tcggcgtacc agcgttggcc ctcgtcgcct cccttccccg gtgccgcccg agtgcggaag
     4321 gtgaaacttt ccccgccgtc ccgccagccc gtctcgtgat cggtcacaac gatcttcgtc
     4381 atgccatatc gggcaacgcg tttccacaac tggtaatctt tttcacgatt tgatgcccca
     4441 tgtgcccgcc atacccgctc gccggtgaca tgcatccacg gtgaccgggg attagggata
     4501 ttggggagga cctcctcgaa gcgcggcgat accgtaagaa acaaccgctc aaaggcatcg
     4561 ttgagtttgc catcggtctt ggggagatag cgggcaccgc cgttatatgt caggtgatct
     4621 tccctggaat cgttaatcgc aaacagggca gacgcattag agcggtaata atcgaccaga
     4681 cccatcataa agagtggcgc cttttcatca ccggcgacca ccacggcagg gcgttgggcc
     4741 tcgcatgtca agtaaggcag cgtcactaac cgcggatggg gcaggccctc ggcataacca
     4801 aacgacactt cgcccacaat cccgccaaga caggccacgt cgatgacaag gcttttttgc
     4861 cacaatcgaa agctgtaggt cacctcaccg tggcaattcg ctgcttccac ctgccaccga
     4921 gcaaccacgg cgtccccttc caccgcgcag ctcatcagct tgtattgcgg ttcgaccagg
     4981 cggccctcgg ctgtgcgaag acgaatcccg ccgcttacca tgggtcgaaa acttagtgcg
     5041 ttgttccatg tcacggtgat gtcaccccaa tgaccgtgct ggggtgtgta acgatacaca
     5101 agctggccgt cgtccccgcg ataccgaaac tcatacgcct cgccattttg gagcacttcg
     5161 atttgataat tcggcgtgag ctgatcgggg aggatcgttt cctctcgcgt ggggaagggc
     5221 aaaacgccag ggcccgtgtg aacccctgtc ggctgaccct caggcaatgt cagtgggcga
     5281 cgcggtcggg gctgaaaggt caacggcggc aacggctcca agtacggtgt gagattgtcg
     5341 aagtagagcg cgcggtcctc ctcattcgtt cccccagcga tttcgatccc ctccacaaag
     5401 ccgccgtcct tcagggcttc cacgtcccgg ggatgaagcc gggtatggac gagaaaccat
     5461 tccttccagt taacgtggcc catttcggcg cgaacagggt gtccttcgcg tgaccttaag
     5521 agaacggtga cacgaaccgg tggtgtggta cggtcgggga cccaagccca gttgtttccg
     5581 taaatccaaa cgttcacgca atcgacggga ccacgaaacg cgacagcttg tgggggacgg
     5641 atgatgacaa cgggttttgg cccgacacct cggtaggtta acttgcccac gtatttgccc
     5701 cagatttgct gctcgcgtga acgacgaaaa tcagccactg catcacgggc ttccaatgtc
     5761 cagccctcca agtcctcaaa gtcgatcaat ggtggccggg tgtcctcaaa acgattggcc
     5821 caatccatct cgtaaggacg tttccccact acttcttcct cttcggcagt tgctctgatc
     5881 gaaccgagaa ggccccctcc ggcgaccagg catgccaaca gcaatcgaat acgatccgag
     5941 gtgaacatgg cgctgctccc gaacgcaaag gccaatttca tcttcgcggg catgctttta
     6001 ctggtgggac gacttgctaa agcaccaccg gccaagcaca atggcttccg gccagccccc
     6061 attatggcaa tgccactggg tgttggggag aggctttaca cggtcaagta tgaggagaca
     6121 ctgtcatgcg acatcattgg agacgaaatc tcagcgtgat gatcatcgtg atcatgtcca
     6181 acttagctgt ctttttcttg ggagccagaa ctgcgatcgc ccaacaggca tcctacccgg
     6241 agcgctgggt atgggtcttc ggctggggac taggccgcga ttcggacgtg acagaggtgc
     6301 tgaacctcct ggatcgcgcg gggcgaagcg gctacaccgg ggcggcggtt tcctttggcc
     6361 tggattccct ttgcaagtgg gacgacgcgc gtttccggcg gctggaagtt gtgaaggaag
     6421 cctgtgcccg caatcacctg gagcttatcc cctcgatctt ttccgtgggc tatggtggcg
     6481 gcattcttgg tcacaatcgt tatttggcgg aggggcttcc cgtcaaggat gctttatttg
     6541 aggtacacgg gcaagaagcg acactcatcc ccgatccacc cgtgcaaatt caaaatggcg
     6601 gtttcgaaga gtattctgga cagcgcttca ccggctggcg ttttcacgat caaccggggg
     6661 aagtgagctt cgtcgataca caggtcaagc acagtggcca agcgtcgctt cgcatggaga
     6721 actttcgggc gaatcaacat ggccacggtc gcgtcatgca ggaggtcaag gtccatccct
     6781 atcggtgtta caagatgacg ctttgggtaa aaacggagga cctccaaccg accagtgcgt
     6841 ttatggtcca ggtgctggca gacaaccgtg cgttggcccc cattggattt aatctctcgc
     6901 caaccatgga ctggacgaag ctcgtcttgg tcttcaatag ccatcgtttc gaggtggttc
     6961 gagtttacgc aggggtctgg ggcggacgaa gtggcaagtt ctggatcgac gattggtcca
     7021 tcgaagagat gggccccatc aacgtgatcc ggcggcccgg tacaccggtc tcggtcaaga
     7081 gcgaagacgg ctcggttgtg tacgaagaag gtcgagatta tcagcgactc gaagacccca
     7141 attacagccc atatcgggtg gaccgcccgg cgccaccgtt gcggttgctt cccggaagtc
     7201 gcatcaaaga tgggcaacga ctccgcgtga gttggtatca cgcccagaag attcacgaat
     7261 ctcaggtcac ggtgtgcatg gcagaaccgg aattgtacga aatcttcgat cacgaagctg
     7321 cactgctcgc aaaacatgtc aacccgaaaa aggtcatcct cagcatggac gagatccgca
     7381 tgggtgggac ctgtgaggcc tgtcgtggtc gggacatggg ccagcttttg ggcgaatgca
     7441 tcacaaagca ggtgcaaatc ctgcggaaat acatgcccga tttggaggtt tacatctggt
     7501 cagacatgct agaccccaac cacaatgccc acggcgatta ctacctcgtc gagggcgact
     7561 tcacggggag ctggaaccac gtgccgaagg acctggtcat cgccgtttgg ggtggcgagc
     7621 cgcgacccaa gagcgtgcaa ttcttcgccg aacaggggtt ctcgcaactg atcgcctgct
     7681 actacgacgc cccaaacctg gaaggtgtga aaggttggct aaacgtcgtc cagggcgtac
     7741 cgaaagtgaa aggcttcatg tacaccccgt ggagaaaaag ctacgatctg ctcgaggaat
     7801 tcggacggct agtttcgggg aagcaataga caaagacagg gcggacgcga cagaatgtgt
     7861 ccccctaggg tagccgaggc ggggaactgc gcgcctccaa aataacggac gcagaggacc
     7921 gcgtccctcc cagacggtgt ccctggcaaa ccaaactgcg gtagtagagg gtcatgtccc
     7981 atcgtggtca agccaaaggc ccgctaagag cctatcccat aacctcccct cgcccgctcg
     8041 gcgggagagg ggccgggggt gagggtgact cgaatcgctg ctggttttct cactcgactc
     8101 cgacgatcgc ttgccctcac cccaaccctc tcccagaggg agagagggac tttttgggac
     8161 gggctcttat ttgccgaaat aatcgggtgc gttgccgggc ttccacttaa tgttacaacc
     8221 caaagagggc ttttgatcgg gcgacaccgg ttgaccggcc agtacggcgt ctaaggcggc
     8281 gcggaggtct ttgccggtca ctgggatgcc gttgttcggt cggctatcgt caagctgtcc
     8341 ccgatagacg agccgttgct ctttgtcgaa aaggaaaaag tccggcgtgc aggccgcccc
     8401 ataacgcttg gccacctctt gggtctcgtc gaacagatag gggaaggtgt aaccccgcag
     8461 cgcgacctcc tcccgcatct tttcgggcga gtcctccggg tagttttcca cgtcgttcga
     8521 gtttatgcca acaacggcca cgcctttggc ttggtattcc ttggccagtg cggccaagtg
     8581 atcggccacg tgtttgacat agggacagtg attgcacatg aaggcaacga gcaaggccgg
     8641 cgctccacga aagtcggaaa gccgaacgat ccggccttct gtgtcgggca aagaaaaatt
     8701 gggagcagga gcacccaagg gaagcatcat cgtgctggca gtgcgaacca tcgtatgttc
     8761 ctccgtttta atcgactacg gacatgaact cttcgttttg cacagcgtac gggcgttaaa
     8821 cgtcccaaca cgcctgcttt gccaattgta cgctgccagg aaagagctac cttctaaacg
     8881 ccgagccggt tttcaacagc ctgcgttgat gcgctcgaaa gatgaggatg aaacgatcag
     8941 ccctcggagg atctcggagg gcgcatccgc ttctcctgca aaagctctcg gatttcgcca
     9001 ctggctatag ccgcagcgaa ggcccctaga tcggcataga tgcgatggct gagaaacttc
     9061 accacccagt tgtgtttcat ggggctaatg gagatcacga tggccccatg ttggtatgcc
     9121 tcccacatct cgatggcggt gcccatcgag gcctgcggca gataggcaac caacaaatca
     9181 atcgtttgac acatcttgtt gtggtagaaa aagacgtccc gcccagtccg ccggtcgtac
     9241 tccagggagt tcctatgatc ggccagcggg tcgtagagat caacctcggg cagatactgc
     9301 tgaaagaggg ccttgatttg gtcacgatag tcctgaccaa cgaggccctt ctccaccatg
     9361 gacccttgca ttacccctgc caagaaggca cggaacctct tggcctctcg ctgagagccc
     9421 tgctcgtagc caggcaaaga tgtcttttcc gttaaagatt ctctagtcgg caagtcttcg
     9481 gtcatcgttc gatttgtcag cggtccttta gcatcacgtg gaacacccgt cgtcacggaa
     9541 cgtccttgat gaacgaaacc ccgagtttga cccggtaaag tttccctcac tggagcgctc
     9601 gtttcagttt atcacagaaa atgcgggcag gttggtgaaa ataggctctc cgtggtcaaa
     9661 acagcgggta tgtttactag ggaactcgca acacgccctc tcacctgcct taaccaaaac
     9721 ctgcctgaac atccaaagct ggagtttcgc gatgcccagg cttcctgccg cttttggtgt
     9781 tattttaacg gctgcgctgt gcatcgggtg ggccatcctc cgctacccac aagtcaacca
     9841 gcaggtctgg gagatggtcc aagcgggacg acaaccatca gcagatggga gcagcggttt
     9901 ccatcggcta gggctggcca tcccggcaaa tatggcggtc gcccaagcgg aacaaagatc
     9961 gacggcgaac cccaccactg tggatcgtgc ttccccagcc gcgaccgaac atgaagacgt
    10021 ggaagccgga ttcgcaatgg ttcccatcac cggatacgtg ggggcgaatc aggccaaacc
    10081 aaaagatgag aagggacctg cccagcaatc acatttggaa aacgtccaag tcacgctggc
    10141 cgtcccgccc caaatggcga tccttagcca acccaacgac gaccgtttct ccgggcatga
    10201 tgagtcagaa acgccagatc ccagcccagg ccaaacagaa tcagacggaa ctggccgcga
    10261 catgatgaat ccacgtgccg aagtgccatc cactccatcg ttggaagaag agcctactga
    10321 gcggcttccc gtacccagat ctcgggagga aagcgcacct tctgtcggcg aaacgaacgt
    10381 cacagccggc gactttaccg gccctgggaa ttccgctcaa cctactgaaa ccgtcaaggt
    10441 aagcagcgtt tcgccaagcg agatggctac cgactcgggc agtcacgacg attatgtcga
    10501 tcccccgttg gtgcccgtga tcagaaacag ctcggccaca gggctagcag cctcgctaag
    10561 tgagacgggt ggctttgagg tggtggcccc ccatgaggcc gttgctcggc ttgcgcccag
    10621 tccgccggtg acgttaccgt ctgacttgcc gctggtcaac atttaccgta accggtggtt
    10681 tgccgccgaa aagccgcagc gcctgccacc ggtggaggag gcctcgcaag actcggacgc
    10741 ctcagccggt atggaacggt actgggatgg agcccctagc attccgcttt atccctcgac
    10801 gggacggtga aaaaacctca ggccgcgcgg ggcgaggcaa gctgccatct cacaagggcc
    10861 aggccctcgc agagcgatag atcgccgccg acgacggcgc cggtctcaaa gccgtaatgc
    10921 acgagacaat cgtggcagcg cggatcttgg gcgggtccca gttgatgcca cggcgttttc
    10981 tcctgccact cggcgaaggt tggataatgc gtatccgtca aaaggtaaca gggagatttc
    11041 caaccacaga tattgcgggt aggacatgcc caggccgcac acggaatttc ccgctttccg
    11101 cataagaaat ccaaatacag tggagagttg agaagccgat aaccttccag tcgtttgcgg
    11161 atggcggcga acttggcgtg gacctcgcag cgcgtcaaaa agagttgcga tgccacctga
    11221 tggtctttgc ctaactcgcg tgccctgccg ggtaattgag cttgcccatt aattgtgggc
    11281 aaacgctgag gttgcccatt taccaccgga taagggtaag ccggcgaaag gagcatgcca
    11341 tcgatgcgga gctcgctgag gtaatcgagt agcacaagaa tttcatccat gtcggtttgc
    11401 gcatagatcg ttgtattggt ggttacccgc cggccatccg ctttgagtct tcggatattt
    11461 tggatcaccg tttggaatgt ccctggtcgt tgggtgatgg cgtcatgcgt cgcttccatg
    11521 ccatcaagat ggatgttcca tgtcagccgc gatgatcgtg gcaggcggtc gatccagcgg
    11581 tcaacaagca gggcatttgt gcacaggtaa atatggcgtc cctgggcgat caaaccattg
    11641 acgagacggt caagctcggg atagagcagc ggttcgccgc cgcagatgct gacgatggga
    11701 gcgtcacact cctgggccga ggcgaggcat tcctccacgg acagtcgctg atccatgaca
    11761 tgatgatatt cccgaattcg gccacagccc tcgcatcgca agttgcaagc aaaaagtggt
    11821 tctagcatga ggacaatggg cgcacggcga cggccggtca accggcactt cagcgcatgc
    11881 caggtcatgg ccttgatcaa cgaccaggga aactgcatcg cgctcgactc cttcaagcga
    11941 tcatggatta ggcaatgcgg gccgtcacat cctcaacatc gattggtggt gccaccacgt
    12001 gcaactccgg caggtcaagc tcggcaagtc ggtcggcgtt catggtggca aaacgagcca
    12061 gggccaaaag cgggaaatag aggggataca tgtggtagcg gagataaaac acgcggggga
    12121 agcccgtccc ggtgaaggcc tcttccggcc aggaaccatc cggcaactgg tgccgcaaca
    12181 aatatcggat gccgcggacc agcgcggggt cactggcctt ccctgccgcc aaaagtccca
    12241 agatagccca ggctgtttgc gacggtgtac tggggccgtc accgcgtagg tgaggatgct
    12301 cataggtagc cggtgattca ccccaacccc cgtcgggcaa ctgatggctg aggagccagt
    12361 ttgccccggc gacgacggcg ggatcgtctt ccggaacgcc caccgcacgt aacccagtga
    12421 tggccagcca ggtcccatag atgtagttga ccccccagcg accgaaccaa ctgccatcgg
    12481 gctcctgggt ccgcctgagg aaggccaccg cccgatctac tgcgggaaac cccacgcgat
    12541 aaccgagtgt ccccaatgtc tccaacactc gccctgtgat atcgggagtg ctcggatcga
    12601 tcatcgcgtt gtggtcggca aaggggactt tggtgaggat cgcgcagcag ttgtcccgat
    12661 caaatgcggc ccaaccgcca tcgcggcttt gcatccccag taaccatgta acgccgcgat
    12721 agatcgcctc attagtccgc ttggcaagct cttccaattc ctgcggcgac attccgcgac
    12781 tcttggatcg ttgccccgtt tggggtgtct tgcgtctatc ctcatcaacg agccgccatt
    12841 gtggggggaa taattgggag ttctgtttct cgtcgtgatt gcactgcgtg gccaagactg
    12901 tgagaacggc agccgtgtca tcacagtcgg ggtagaaagg gttggcatgt tcaaaacacc
    12961 atccgcctgg cgggacgttc acggtgtgag accaatcacc tggcgtcaga atctgatgat
    13021 cgaggagcca cgccacggcc ttttggacct gcccatcgtc tgtggatagg ccggcagaca
    13081 tgagagcccg cagggtcagg gcagtatccc aaacagggga gagacacggc tgaacccggg
    13141 cactggtttg gtcttcggtg tcgatgagca attcttccac gcggcgccag gcctcccgca
    13201 tgacgggcga atcctgcgaa taccccaggg cgtgcagggc aatcaaactc cagaccatcg
    13261 gcggatagat ggccgccagt ccgtgactgg cctccatgtg ttccaacatc cacctctctg
    13321 ccgcgcgcaa agcacgcttg cgcaagggga ggaatcccca gcattcggca tacttcagac
    13381 agcgatccac catctggaag aataaggacc aaagatcgca gcccccatgt gcccctgaac
    13441 cccgggtcaa tggccagtct tccggtcgag tcaaaaagag ttcgcggatt cctttctcag
    13501 gcggtaacgt gcgaacgggc ttcatcgccc acaacacgga taaaggaacc accatcgccc
    13561 gtgaccaagc gctcatctta taaaggttga tgggaaacca cttcggaagg aggacgacct
    13621 cgggaggcac cgccgggcaa atgtggtaag gtatttgtcc taaaaaggcc aggtaaaaac
    13681 gagtaaaact gttgacggcc tgagcgcccc cgtgctcaag aatggcctct gcggcgcgac
    13741 gcaatcgtgg atcactgggg gaatatccag ccagtttgag ggcgaaatag gccttgacgc
    13801 tggcgcttac gtcgatcgga ccaccgggat agagcgacca gccgcctttt tctgattggg
    13861 tctccagaag gcgttcagcc agccgcgcta ccaatgggga gttttctcgc ccaaaaaagg
    13921 ccaggatgag gatcgtttcg ctttgcagga tgctgtcgcc ttccagttca ccctgccaat
    13981 agccttccgt atgttggcga ttcaacagat gccagcgggc aagagaaacc gctcgccgaa
    14041 tcgccgatgg cagggatacc ccatcgccgg cgggctgcgc tgcgcggtca ttcaagcatg
    14101 atctttttac ggccccacat ccgtgccgag aatgttccat ggccaatgct tcctttgtga
    14161 cgcactccga gacgtccaag tcaatagata gattaaagga gacgcgcaaa gttggcaaga
    14221 cgaacttgac ggacgcgaaa cgcgctgggt ccgcactagg attgcgcctt cggtccctca
    14281 ttccccacaa tcgccgccta catgtcaagc caggatgagg gccatcaaat cgtcggcaat
    14341 tgccaaggat cccgtcgcgg ttcgcggcgg attccgtcca accactcgtt ggcctcggtt
    14401 ggttcgacaa accgccagtt cttggggtcc cacttcagcc gttttccggt ccaataagcc
    14461 aggttcccaa gatggcaaac ggtgacggat cgggcaccaa cctcaacggg gcaaatcggt
    14521 tgccggtttt cacggatggc attgagccaa tcacgatggt ggcccggcga ctcatacaga
    14581 cgcacttttt cgctctcttt gagcggttcc ttgaccacct cctcgggcca actttgaagt
    14641 ttaccgcgat cgacatagat gacgccgtcc gtacccacaa aattgactcc gccgcggcct
    14701 tcgtaaccac cgtggatcag ctcgacacca ttctcgtaga tgaagcgaac gcccgttcct
    14761 ttctcgggtt gctcgggcgg gatgatctcg acagggcccg agtcgtccat gccgagtccc
    14821 cactgggcga tgtcgaaatg gtgagcaccc cagtcggtca tccctccgcc cgagtactct
    14881 cgatatgccc gccatgccgg gaaatggtca tgcatacctc gcggactgag gatcgaatta
    14941 tacggccgca tgggtgcctg ccccagccat aaattccagt ccaaccccgg ttccatgggc
    15001 tcctccggca gatcgcacca cttgctcggt ccgcccacag aggcgtaaac ctgcttgatc
    15061 ttgccgatgc gaccattccg cacagcctcg cacgcggttc gaaattctcg acccgaccgc
    15121 tgctgactcc ccgtttgaaa caccacacca tatttgttga ccacctcgat gcacagtttg
    15181 gcttcggcga tcgtgagtgt gaggggcttc tcgcagtaga tatgcttctt ggccttgcag
    15241 gcatcgacaa tgggatgggt gtgccagtgg tcgggtgtag caataactac ggcgtcaata
    15301 tccttgcggg caataagctc acgataatcg ttatacgcgg cgcaatcttc gtttccgtat
    15361 ttagcgttca ccttgtcccg cgagaaattc cgccgcgtag tatcgacatc acaaacggcc
    15421 accacgtgga catctttcat ccccaagaag gcgttcaagt ggtattggtt ctgcttgccc
    15481 atcccaatga agcccagggt caatcgttcg ctcggtgcca ggcgttcttc gcttcccagg
    15541 gctgttcggg caatgatggt gggcacagtt agtcccaccg ccgacagttt gaccatctcc
    15601 cgccgcgtca tcatcccacg tggttgtttc atcgtcgtta cctcccgatt gtttgctata
    15661 agcccccatc aaaaaaccca cccggcgacc cctcctcacg gccttagtgc ctcccacatc
    15721 cctgggcttg gggcgcaagt tccctgacaa aatggaccac agatcactgc gcaagttaat
    15781 cactcagata ggcaaaaaca agtggcgccc gcccgaagcg gcgttttcga aggcctcttg
    15841 cgcccaacgc actgctaggc atgaaaaagg ctcctccctc cggggtccga aacattgggc
    15901 attttcctcg gaaggtgagc tatagtttca ttagatgcta gacggcgaag tcattcatga
    15961 acgttctagg gacaaaaaga gatcaattga gccatggaac ggaacccagc acgacgccca
    16021 tcacagtcgc cccaagaaag cgagcgaaac gaaatcagct ccgccgaaat catggattat
    16081 ttcttggata aggtggagga gctgcattct tctccggcgg tcatttggcg ggtccttcag
    16141 cttttgaaag accccgagtt cgacgttcgc gatgtggaac tgctgttgga agccgatccg
    16201 gccctgtcag ccgccatcct acgattggtg aacagttcgg cctacggact accgcagaag
    16261 atcaccagcc tgcgacaagc cattctcctc ctgggtgcgc gttcccttcg cctggccgtc
    16321 ctcagctttg gtctggttga tcgcttgacc aaaggaacgc ccgccaaagt gtatgaagat
    16381 ttctggcgac gcgcactgac aacagcctcc gccgcggctc gcctcgccag gacctatcgg
    16441 accacaaatg ccgatgaagc ctattgcgca ggattgttat cggaactcgg cgtcctggca
    16501 ctggcccaag tcgatacgcg gcgttacacc cgggtgtatt tgacccaccg gcacggcgag
    16561 gacctttgcc ccgcggaaca ggcccgctat ggctttgatc acgccgccct cggcgaacgg
    16621 ctgctccttc gctgggggtt gccccgaccg cttgcccagg tggccggttc ccaccacctc
    16681 gtggaaggca cgcccgaggc ccttcacgcg gtcgtccatg ccggttcgct tctcgcggat
    16741 gtcctttgga tgccgcagtc cgtgctcctt ccccacgccc ggcagtggtt gtccgaccgc
    16801 ttccagatcg acctggatgg cttcatcacg ctggccatgg attgcaagca ggacatcctt
    16861 gacaatgccg cgatgttctc cattcacttg gaaggtaaaa tcgacatcga cctgctgcgt
    16921 cgggaggccc agcgacaatt caaacaggtc gctctcgaga cgtctctcga gtacgacgcc
    16981 atgaccgccg tgctcaatca agaaatggga atctgaagag acgcatcccg cacggccaac
    17041 ttgcgctacg ggcttgatct agcgcccgtt tcttctcagc ttggggtggc aaacgcgaaa
    17101 gtgggcaaac cccacgccat ccgtgcttgc tttgttccga tggagaacgt atgataaaga
    17161 ggtcataaat gagactgagt ctcaataagc cctcgctatt gagactggga caaaatacgg
    17221 catggcggat cggtcgttag gcatcggatt tgtttgggca aaccctcggt gcggtcggct
    17281 gtcattttgg cgtgacatta tctccatccc atgaggatat catgatcagg ttcgcttggg
    17341 gaaaaaacgg tttctggcgc gaggaaaggc cttcctccca agtggtgcgg cttgaagagc
    17401 ttcccgcggg agaaagggcc cagatcgttt ctctcagcgg acccgaggat gttgttcacc
    17461 gcttagaaga gttggggttg aggccgggaa cggtcatccg cgtctttcgt cctggtaatc
    17521 cgtgcatcgt gtgtcttggt gggagcaagt tgggcgtgcg ggcggatggg cacgtccaaa
    17581 tcctcgtgag ccccctgcct ggaccaccgc ggtgccgtca ccgacatggc tggggctggc
    17641 gccgtgcggc gatcgtatcc cccaccgcgc cggggaagga cgtccccgac cctctccctc
    17701 cggcaaattg acggtcctgc agggcgataa cctgatcacg acgggaatcc ccatattgat
    17761 tgcccgctca ttttcaccac cgtcgtgttg tctaaagaaa gatcgcacgg tgctccgctt
    17821 gggtgtttgc gacgaagttt atggctgttt ccgcagataa acggtgcatc acggttgccc
    17881 tggtgggaaa tcccaacaca gggaaatcga cgcttttcag cgcccttgtg ggggtttatc
    17941 agcgggtggg gaactacccc ggtgtgacag tagagaagcg gacaggccga ttcgaattcg
    18001 ccggacaatc attcgaggtc accgacctcc ccggtctgta cagtttagcc ccacgatccc
    18061 gggatgaact ggtggccgtg gatgtcctgc tgggacgcga gccgggggtg gagccaatcg
    18121 atatcatcgt ctgtgtggtg gatgcatcca atctagagcg caatctttac cttgtcagcc
    18181 agcttttgga gctcagtgtc cccacggtca tcgccctcaa catgatggat gtggccgaac
    18241 gtcaaggcat tcagatcgac agcgacgagt tggcccggcg actgaatgta ccagtcgtgc
    18301 cgattcaggc ccatcgcggc ttgggcgtgg gcaggctacg ccaggtgttg gccgagctgg
    18361 ctgattcacc ccgcaccatc acgccgctta caattttgcc gcaggccttt ggggaggcct
    18421 gtgagcaact tttgagcgaa ctggagaagc gagattggct gaagtgcctt agtccgcagt
    18481 cgtcgagcca cctggcgcgt ggtgaggcaa ctttttccca gccgaatagc ccgctgaagg
    18541 ccccggctta tcggttttgg gccggacgtc tcctcctgga ctcaacgggg tttctgcaaa
    18601 aggccatttg gggggaacgc gctaaagagg taaagcggtt ggttgaggcc gctcgagagc
    18661 aattacgccg ccagggtttc aaagtccccc aggatgagac ccactttcgg tatgagtgga
    18721 ttcgcacgat cctggacggc gtggtggtca tgccggccga gagcagcccg acgttaaccg
    18781 atcgtctcga tggcgtcctt acccatcggg tgtggggcac gctgatcttt gttctggtca
    18841 tgctcttgat gtttcaggcg gtctttcgtt tggcggctcc gcttaatgag gccatcgaca
    18901 gcgcgattca ggggatcgca ggttggctga cggccgagat ggcccccggg gccttccgca
    18961 gccttttggt ggatggggtt atcagcggtg tgggggccgt gctgacgttt atccctcaga
    19021 tcgccatctt gtttcttttt gtggcggtgc tcgaggattg cggctacgtc gcccgcgctg
    19081 cattcctcat ggaccggttc atgtcgcgga tcggattgag cggcaaatcg ttcattccca
    19141 tgctttccag tttcgcctgc gctgtgcctg gcatcatggc cactcgcacc atcgacaacg
    19201 agcgggaccg attgacgacg atccttgtgg caccgctgat gacgtgctcg gcacggctgc
    19261 cagtctatgc cctgctcatc gccgtgttta ttcccgacca aagatacctc ggtggtctgt
    19321 tgggactcca aggactggtg ctggcgtcgc tttacgccct gggaattgtg acggccgttg
    19381 tggtggccat cacattgaaa accacgcttt tccgcggtcg ttcgccgacc ttcgtcatgg
    19441 agcttcccag ctacaaatgg ccgtcgccgc ggacgatcat cctgagggcc tgggaacgtg
    19501 tggtcgtctt cgtgcggaat gctgggacga ttatcgcagc catctcgatt ttggtctggg
    19561 cggccctgta ctatccgcac gatctacgca gcctgccgcc cgagttactc cgccggcaac
    19621 aggaactcat cgtcctgcgg gataccttgc cgccgggatc ctcgcagcga cagcggtggg
    19681 aaacggaatt ggagcaaatc gaacggcaaa taacggcggc ctatcagcga agcagcttca
    19741 tgggacgcct cggaagggcc atcgaacccg tgtttcgacc ccttggatgg gattggcgaa
    19801 tcggcacagc cgtcatcgcc tcctttccag cccgcgaggt tgtcgtggcc accctgggcg
    19861 tgatgttcaa cttggacgcc gatgtagcgg aggccaacca gggcccctcg ccttttcacg
    19921 accgcttgcg atcggcggtt tgggaaggca cagatcgtcc gcttttcaat atccctgtgg
    19981 cgctttcatt gatggtcttt tatgcgttgt gcgcccagtg tgcagctacc ttggctgtca
    20041 tcggacggga gaccaatagc tggcgctggc ccattttcac attcgcgtac atgacaacct
    20101 tggcctacct cggcgccctt gccacgtacc agattggcgc tagaattatg acaggcacat
    20161 gattcgttct cgctgattac attatacccc caattggtcg gatcgaacaa agggtctccg
    20221 cgatgttgac ggcagtttgg caagacgtcg cgacgtggtt gtccattgcc ctggcggcaa
    20281 tctatctcgt ccatcacttt cggcggcagc ctggaatggg ggcctcacgg tgtgggggct
    20341 gtacgcattg cccaatgagt gacgcattac aagagtgggc ggctgcgtgg aggcagacga
    20401 accataacgg ccaggatcag taagtagaga tcgacacccc gcccgccatc ctgattcgaa
    20461 ccttatctga aactgtttgc tcccgcgatt caggtgaccg gtacctggcg tcaacgtggg
    20521 acggcgagga cgcggtccct cccaattccg cagcgggagg gtcatcgtcc ccgatgacgc
    20581 tggcatctgg taacacggac gcccgttgca gacaagcgct taacgttggg atgaagaata
    20641 ccacgggcag gatgcccgtg ccacgcaatt tcagttcgcc ttccgggcgg ccataacgtc
    20701 aaccgtaggg gcaaccccct gtggttgccc cgtttgccgt agcgccgcgg gtttacgttt
    20761 ggccggttgc ccagatcgtt tggtttccac cacgatccgt aggttgtttg cgttccgggg
    20821 caggcacagg ggcctgcccc taccagtttc aatgggctcc ttctcgcgca ggtccacagc
    20881 tgcccggtag cttgcgtctt tttaagaaca gatggactct gcccggcatg tcaccatctc
    20941 cttgacgtcg taaacacgtc taatcatgat gatcagtttg tgcacggtcg tgatggccgt
    21001 gccggttggc ttccgtgatt gggttttgtg agaggaggtt ttgttatgcg cgttctgttc
    21061 gcgataagtt tggtgtttgc cattgtcgga tgctgcgctt cctggacgag tggcgaggaa
    21121 tgggtcagtg gcaagatttg ggaggaaccg cctatcgtaa cacctggcga gtgcggttcg
    21181 ccgccgtctg acgcgatcat cctcttcgat ggcaaggacc tgtcgcagtg ggagggcgga
    21241 gagaattgga agatcgaaaa cggctatgcg attgcccaaa agaccggcat caccactaag
    21301 gaacagttcg gcgattgcca gcttcatctc gagtttgcca cgcccgaggt catcgaaggc
    21361 tctggccaag gtcgcggcaa tagcggtgtt tatctcatgg gacgttacga ggtgcagatt
    21421 ctcgactcgt acgagaacaa gacttatttc gatggtcagt gcggggcgat ctacaagcaa
    21481 catccgccac ttgtcaatgc ctcccgcaag ccgggcgaat ggcagacgta cgacatcatc
    21541 tttacgggtc cccgtttcga tgaggcgggg aatctcctta agccagccta tgtgacggtc
    21601 atccataatg gcgtggtggt tcaaaaccat tttgaaatcg aaggttccac ggcctggcac
    21661 gttccgccca agtacgagcc gcacggcgaa aagggcccga tccaccttca attccacggg
    21721 aatcccgtca ggttccggaa tatctggatt cgggagatcc gagagttgac ctaccagcaa
    21781 aaaggccagg agcctgaaaa gaaagaaccg gaaaagaacg aagcagagaa ggccgaagcc
    21841 gaaaagacgg aaccggaaaa gaaggctgaa aacggccaga atcagtgacg ttatcctggc
    21901 gtttccatcg caacaatcgc cagcccccat cgtaagatgg gggtgtcttt tttcccggca
    21961 ggaaatgccg tcgcaaaaag tggcaggtcg ggcgtgataa aggatcatgg gctggggtag
    22021 gcgaatcaga tgatgggagg accgcgcggt gtggtggccg ggctcgatga tggaatatca
    22081 aacggcacgt caccggaggc gatggcgcgc gttcttccca ttgtccttcc acaccgcagg
    22141 tgtgtttcca aattggccat cggccaacca cgaaggttgt gcctaccgtg gtgcggcggg
    22201 caccggcgag cgacaaatcc ccgaaccttc gctcggctac tgtaccgtaa atacaaggag
    22261 gggtatattg caggttgatc gcgtttccca ccgtgccaca gtaagaccgg cggggagcgg
    22321 ggtgcgagta atctttttgg gattgagtat taagcagcta tgtcgatccg agtcgccatt
    22381 ttgggtgcca caggttatac agccgttgaa ttagtgaagc tccttctgcg ccatcctgaa
    22441 gtcacaatta cggcgctgac cagtcgtcag gagggaaatg cccctattgc gagtgtccat
    22501 ccatcccttg ttggtcggat tcaggcgaat ttggagaatc tttcggcggc agaagtggcc
    22561 gctcgggctg agtgcgtttt tagctgtctc ccccattgtg ccagtgcaga ggtcatcccc
    22621 gaattactga aagcagggtg tcgcgtagtc gatttcagtg cggactatcg gctggcctcc
    22681 gcccaggtgt acgaggactg gtacggccat cggcatcccg acccggatcg actgggcaag
    22741 gtgccctacg gtctgccgga gttgtttcgg gaggcaattc cctcggccga tctggtggcc
    22801 aatcccggct gttacccgac gtcggccatc ttgccgctgg ccccgctgct gaaagagggt
    22861 ttgatcgcgc gggaggatat tattgtcgat tctaagagtg gcgtctcggg ggccggtcgt
    22921 acgcctaagt tgacgaccca tttcccggag tgcaacgaaa gtctttccgc gtacaacatc
    22981 ggtcggcatc gccatacgcc ggagatcgag cagatccttt ccacggtggc gggggcggag
    23041 gtgcaaatcg tctttacccc gcatctggtt ccgatggatc gcggtatctt gacaacgact
    23101 tattcccatc cgacgcgaca ggtcaccgag gacgaagtcc tggattgcct acggtcgttc
    23161 tacgatgacg aaccttttgt gcgggtagtg gatcaccttc cggccactaa ggacacatcg
    23221 gggacgaatt tctgtcatat tacggcacgg attgtccgcg accggatcat caccgtcagt
    23281 tgcctcgaca atctgatcaa gggagcgtcc ggggccgcgg tgcagaactt caacctaatg
    23341 tacggttttc ccgagacaac ggccctgcta tgacaataga ggccgacacg gcgggctaaa
    23401 ccgccttttg agatcagacg tctcgattgt taacaagcta gcgacggttt cgcagtagaa
    23461 gaaggccttg cccatggcag tacgtgttcc taagggttat cttgttgccg gtgttcattg
    23521 tggattgaag cgcgatttgc gcaagccgga tttatcgctg atcgtttctg aggtcccggc
    23581 cacggccgcc ggtgtttaca cgcagaacat tgtttgcgcg gcaccggtcc agctggatcg
    23641 ccgacggacc ccgagcaacc gcatccgcgt ggtcgtcatc aattcgggca atgcgaacgc
    23701 ctgtaccgga gaacgtggcc tccgtgacgc cgaggaaatg gcgcggcttg ctgccaaggc
    23761 cgtcggcgcg gaggaagatc aggtcttggt gatgtccacg ggtgtgatcg gcgtgtattt
    23821 gccgatggaa aagattgctc acgggatcga attggcggcg gcccgattgg gcaacgacga
    23881 agaggccctc attatggcgg cccgcggcat gatgaccacc gacacggtct ataagctttc
    23941 cgggcggacg ctttcccttg cgcggcgaga aatccagatc acgggcatgg tcaagggtgc
    24001 ggccatgatg gggcccaacc tggccaccat gcttggggtc atcttcaccg atgccacact
    24061 ggaggtggat accgcgcaac agttgctccg cgaggtggtg gatgacacct tcaactgcat
    24121 taatgtcgat gggcacacta gcaccaatga cacggtattg tttcttgcca atggtgcggc
    24181 ggggggacca cccctgtccg gcgatttgct agacgatttc aagcaggctt tccacagtct
    24241 gtgcactgaa ttggctcgca tgatcccggc agatggcgaa ggggccacgc acctcatcac
    24301 catcgaagtc cgcggctgtc gtacccgtgc ggatgccctt cagattgccc gggcgattgc
    24361 aaatagtcct cttgtcaagg ccgcgatcca tggtgccgat cccaattggg ggcggatcgt
    24421 ttctgccgca gggtatgcca atgtgccgtt tgatcctaac aaaatgactc tttatctcaa
    24481 cggcacgtgc ctttatcggg atggaacgcc agtaaagttc gacgagatcg aggtctcgaa
    24541 ctcaatccgg gcagaacgcg aaacccgcat cgagctgatc tttcaggaag gctcggccag
    24601 tggccgcttt tggaccaccg acctcacgcc ggagtacgtc cggctaaacg ccgagtatcg
    24661 aacgtgacgt attccctgcc agaaacgacg cagccctatc ggtgacggtc ccgacgcgcc
    24721 tgtcaggcat gtcgcggtgt tgtgggaatg gcttgagggg tgggggaatc actccaggcc
    24781 acctgaaccg gctatgggga ttcttgctcc ggtaacttga tcaatccgcc gttttgggag
    24841 gaatcgcggt tcgatggaac agtgcacgcc cacactgtcg cgacttccat cgacattcag
    24901 cccaagactg acaaggaagg actctccaat ccgcgtgagc gagaaacttt ggccgatgtt
    24961 gctggattcg cccaggtcaa tcgaaacccc caacgtggac atccatttgg gcgacatcca
    25021 gtaggagtag gaaaggaaaa ggacggtgct atcgataggc ccttgaagga tccgcaatcc
    25081 aacgtaaaga tcgccacgcg gtgggcgact gagcatcccg ccaaatgtta caagtttctg
    25141 acccgaatcg aaaaagtcga acagacccgt cgaggccagc gtcaatcggt cgccgacgaa
    25201 ccagcgggcg tcgtagtcga gaagtccgac gaatcggcca aagtcatccc gggaggaatc
    25261 gggaaatagc gagaaatgcg tatcaaacgt gatccaatcg acgatgcgtc gtgaaccggg
    25321 catgccacgc ttggtttgcc atcgctgttc cacccccaag cgaatgagcg tcaagtcatc
    25381 agcaatttcc atactgggtg atgtcaccca agaggccagc ccgctgcgta ccgcgtagga
    25441 tcgctcatca aaggcgtaag gggctggtgg gccaccaaag ccatttgtcg gcccaaaggt
    25501 caacaccccc agtcgccgcc ggaaggcctc gatggcatcg tcatcgagcg gctcgtaaag
    25561 cggcagggcg tcgaggtcgc ggttggaact ggcccaaaag gcctcagcct tgaagtttat
    25621 tttgtgagca atcccatgaa cgttgaatag ctcgctctca atgtagggat tgaccgccca
    25681 aagggggaga ttggcgcggg caccaagctg gacgtaaccg cgctggaggg aatccccaga
    25741 ccggtcttcg ccccaatgcc ccagttcgcc cagtgcatag gggacaatct tgatgacgcc
    25801 gagttgaaag ggccagtcga tctcttgccg cgtaatcatg cgttcgctga acgtgctgag
    25861 gggatcaccg gttagagacg acgtctccca cggaagatag gaaaaggcgg ccagatcttc
    25921 aggattacta ggggcggagg ccgtccgaaa acgtgccagg ccaatgctgg tgtgggcata
    25981 ccaggtgaag gcgtcattga agaaggactc gcccagtttg taatgatcga accgcggcag
    26041 ccaatctgtt tgggtgaaga acggattgag tcgaacagaa gccgagaggt cccacgaggc
    26101 gttcccgtaa atccgtttga gcgagagact ggttgtctgg tctttaagtt cgtcccattc
    26161 tcgctcgtag tattgctcca ggaaattgcg gtccgagatc agccccacct cgccggtcaa
    26221 ctgatattcc tcggaaagat actgtcggtg ttgccaaagt aggcggtagc ggtagtcggc
    26281 ttctggggcc aaggcccgtc gtccccgccc caggttgtcc agaccgttat cataaatccc
    26341 ccagaagtcg gccaagccgg tcgcgtaacc gggtaatccc aaaaatccgt cacgcgaata
    26401 ggtcagcgat gttccgtgtc ccaggccacg ctcactgagg taatctaggc tcaggctcca
    26461 atccattcct tccggtcgat tgcgaatgcc aaaaagttga aaggcgtccc agttggtcag
    26521 gacctgcgtg ccaaagatgc tgtcgtgccg aaggcgaatg cggttcacat agtacgtcgg
    26581 cttggtcaag tccccggcca ggacaggcca gtagaagatg ggaatattgc gcaagtagat
    26641 gaagttgtct cgggccaggg cccagggacg gtgaatcacc gccggttggc cggtttgcgg
    26701 gtcaatcacc acctggcgtg tcgtggggtc aagcaccggc tgaacccggt cctccacgta
    26761 ggcgaactgt gtttgcagtc gataaccggg aacgcccatc cggctcgatg tgacgaagag
    26821 attttgggcg aagaattgcg tctcgttcat ttgttggagg atgtcggcac gaagccggag
    26881 gtagccctcg taatcgggaa tccgtgtgag caattcggcg ttgaggatca tccctgcccg
    26941 ccgcgccaca tcgtaataca tgcgatcggc atagatgatg cgatctccct ggcgaaagac
    27001 gatgttgccc tcgaggtaca gttccagagg ccactgctgg ggtgtggtgg cctcaccgct
    27061 gaggttgggg gcctcgtcgc ccgtgtacca aatgacgact cggtcggctg agacgtcaag
    27121 ggttccccag gggcctggct cttcgacaat gagattgatc ccggcgtcaa tcacgccgat
    27181 ccattggcca ccctgtgggg ccggaaacca ttgggcctgg atggggacat caccgcgggg
    27241 gaagacccga atccgtcgca tggcaccttc cgaagcacct cgcggcgaag gatcagaaag
    27301 gaattcattc ggtcggagga cgctttgcgt cagcccttcc gtggcagcgt tgcgccgcgc
    27361 catcgcccga gcaaagatgg ccggataagg ctcgggttca ccggcctctt ctttgacctg
    27421 aacgttgaca tcggcggtgg tgtacaaccg gccaaaccag gtcttgtcct ggatttgtag
    27481 ccgatgttgg ggatcgtccg agaccacttg ggcacggccc tccaaataga tgatgacctt
    27541 tatcaggcga ccggtccccg atgcatgacg cggcagggcg aatgccgatc cggatcgatc
    27601 agacgtctcg tcagggattg gggccagaag gagtggccct tcttccaagg ccggggccgc
    27661 tacgccaggt gtgaccggat ctccgaggcc caccgacggt tcggcctgcc gatcgatcca
    27721 gattacggac tcccgcgcgc ggaggcttac cgatccctgc cgaatctcgc accacccgcg
    27781 gagaatccac acctcgtagc tgcccatccg ccagtggttg cccgccgcgg ccttgatgtc
    27841 aatagggaaa ctcctattcg gcagcgggaa cgagacatcc tccgcgaaga cccttccagt
    27901 ggcaaaagcg gtcacgaaaa tcgctagcag ccacaggccg ttccacgtca tcgacaggac
    27961 gaacgccagt gccgccgcgc gatcgcagcg ctccccaagg ggtacgaccc ctacggtccg
    28021 ctggacccat ctccagatga tccgaattgg tgcaacgtcc ttagtgctca gagttggcga
    28081 ttccttgccg atgccgaatt taccgtcagt ctttgccgag gccgcacccg aggcacaccc
    28141 atcgcggcct ggaatcacga gctcaccccc actgaggtag ggattgcgga ggggagatta
    28201 tagtggaaca agtccaatga cgacaaggca acttctttga gccttagagc ctcccccata
    28261 atctcctctc actagttcgg cgggagaggg tctgggggcg agggtggctc gaatcgctgc
    28321 cggttttctc aatcgatgcc gatgttcgct tgccctcacc ccaaccctct cccggaggga
    28381 gagggggctt tttgggatag actcttggca gacccctgcc gacggcttgt cggttgcttg
    28441 aggtgcggac accgcagctt atccatttcg cggaatcttc ttttcggcag gcggggaccc
    28501 ggtcgagggc aacgtttttt gcttcactac cagaaatttt gaggaaatca tggtataaat
    28561 ggtgttttca gtgtttggga cgggtttggc gcggatcaac atgtcgaatg aacggccgac
    28621 ggccgagact gacaagatag gtcgttgagg aaggaacatt ctatggactt gaaacgtgtg
    28681 acattgcccg agaaagaact tccttcggaa tggtacaacc tgctggccga tttgccccag
    28741 ccactgcctc cgccggttca tccggtgacg aaagagccgc ttgatccggc tgccctggag
    28801 cggatttttg cccgggaatt gatcaagcaa gaggttagcc aggagcggtg gatcgggatt
    28861 cccgacgagg tccggcggat ttatgccatt tggcggccct cgccccttgt ccgtgcgcgg
    28921 aatttggaga aggcccttaa gaccccggca cgcatctact ttaaggacga aagccacagt
    28981 ccacccggca gccacaagcc gaacacggcg gtagcccaag cctattacaa caaaatccag
    29041 gggattcgtt gcctttccac ggagacgggt gccggtcaat ggggttcgtc gctggccttt
    29101 gcctgctcct tcttcggctt gcagtgcaag gtttacatgg tcaaggtcag ttaccatcaa
    29161 aagccctacc gccgggtcat gatgcaactg tggggggcca gcgtgacgcc cagtccgtct
    29221 gaggagaccg aaagcgggcg aaagattctc gaggcggacc ccaattgccc cggcagtctt
    29281 ggcatcgcca tcagcgaggc cgtggaggtc gccatcaaga atgatgatac caattacaca
    29341 ctggggagcg tgcttaacca tgtcatgttg caccaaacgg tgattggcct ggaggccgaa
    29401 aaacagatga aagaatttgg tgactggccc gacgtcgtca ttggatgtgt cggcggaggg
    29461 agcaacttcg ccgggctggc gtttcccttc ttgcgacaca aaattgccgg gcaagacatc
    29521 cggtttgtgg ccgtggaacc aaaggcctgt ccgtccctga cgcgggggat gtaccgctac
    29581 gacttcgggg acacgtcgat gttgacaccg cttcttaaga tgcataccct gggccacagc
    29641 tttgtgccgg cgggtatcca tgccggggga ttgcgttatc acgggatggc accgatggtg
    29701 agctttgcgg ccaagctcgg cctgatcgag gcgattgccc ttcaccaaaa ggaatgcttt
    29761 gaggccgccc gcctgttcgc ggccaccgaa ggcatcatcc cagcccccga aacctctcac
    29821 gccattcgcg gggcaattat tgaggccctc aagtgcaaag agacgggcga ggaaaaatgc
    29881 atcctcttta atctcagcgg tcacgggatt tgcgacctcg gggcctacga gcgctatttg
    29941 gcaggtgagt tggaagactt cgagctcccg caagaacaga tcgaacgagc gctggccgag
    30001 ctgccggttg tggagtaaga gcctatccca aaaagccctc gccctccggg agagggttgg
    30061 ggtgagggca agcgaacgtc agaatcgatt gagaaaaccg gtagcgactc gagccgtgct
    30121 cacccccgac tcctctccca ccaaacgcgt gaggggagat tatgggatag gctctaaata
    30181 agcgcggcga acccctacaa tacgtggtgt tcgcttatac ctcggggtgt ggtttcgcgg
    30241 attgtcatct ggagggaaga acaccttatg ggtcctcgac gccgtcacac gatgccgctt
    30301 gggcggatga tagcttgcca tttgatgggt tatttggcgg cggtgctgct gtgggggtca
    30361 gccctcgccg aaaagattgg cccctgggag ctcgagacgc tttatcaacc gccccgggca
    30421 gaatggggcg aggtcaagga tggcatccgt gaggtctatt acgagggtga accctaccag
    30481 gggcatgcga cgcgggtcta tgccgtctat gcgcagccgg aaggcgaggg gccgtttcca
    30541 gccatggttt gcgtgcacgg cggcgggggc accgcctttc ccgagtgggt tcggctgtgg
    30601 gcgtctcagg gctacgcggc catttccatg gatttggcgg gctgtggtcc cgaacgaaaa
    30661 cggctctctg acggcggacc tgaccaaacg gatgtctcga aattccgcga tttcgatgaa
    30721 gccactgtca cagacatgtg gtcataccac gcagtggcgg cggtgattcg cgggcattcc
    30781 ctcttgcgaa gcctccccca aattgatgac gaccggattg gggttaccgg catcagttgg
    30841 ggtggatacc ttacgtgtat cgtggcaggg gtggatcatc gcttctcatt ggccgtcccc
    30901 gtttatggtt gcggttttct ccacgagaac agcgtgtggc ttccccgctt cgagaagatg
    30961 gcgccggagc agcgcgagcg gtggatccgc ttttttgatc cttccaacta tctaggccgg
    31021 gtcaaatgcc ccattttctt cctcaatggg acgaacgatt ttgcgtatcc tctggatagt
    31081 taccgcaagt cgtatgaccg ggtccccggc gagaaactca tccgcatcga ggtccgcatg
    31141 aagcacagcc accccgacgg ttgggccccg caagagattc gcttgttcgc cgacagcctg
    31201 ctgaaaggtg ggacgccttt gccaaagctg acgccccttc ggcgggatgg aagacgggtc
    31261 tgggctgagg tgacatctca gacggccctc cgtgaggctc atctcaatta cacgctcgat
    31321 gcgggacgct ggcaggaacg gcagtgggta accgtccctg cacgcctcga gggtcaaagg
    31381 attgtcgccg agttgccaga accgcaaccc acggcgtttt acttagatgt ggtggatgaa
    31441 cgcggtgcca tggtttccac gccgccagag atcctccgct gatcttacgg atataagact
    31501 gcgcgacacg ctctgggttg caatggaggg tcacgcgccg cctcggccgc aaagacgccc
    31561 acgattgcct ttgcacaagc ggtgactact ggttttccgc ggccgaactg actatcctag
    31621 tgtcgcgggt attggcagag attttcgcga caacttcacg ggaacaaggt gacgccatga
    31681 tcgatttcga tgctttctca cgccattcac gacgcgatac cctgaagggt ctggtggcag
    31741 gtggtgcgat gatactggga ataaagggct tcggacacat ggggtttttc gccgaagagg
    31801 tgaccggcgc cgcgcaggcc ctcgaaaagc tcggctggac ggtggcttgc cagctttaca
    31861 catttcgccg gtttcctttc tatgaggcca ttgagaagat cgccgccctg ggcattaagg
    31921 ccgtcgagcc gtgctttttc ctggcgttgg acaagggtca gccggggttg gtgacgagtg
    31981 aggccctcac tccggaaaag cgggccgaga tgaaacagcg gctggccagg ttgggaatga
    32041 ccatgccgaa cttttatgcg aacgtcggtg gcgacgccga gcaggccaaa aaggtcttcg
    32101 ccttcgccaa ggaaatggga tgtcaaacca tcgtggccga acctccgcct gaggccctcc
    32161 ccatgctgga tgaactgctc gcggaatacg gtctgcgtct ggccatccac aaccatccta
    32221 agtcgccgca gtcgcgctac tggcacccca agaatgtttt ggaggcggtc cgggaccgta
    32281 gcgagcgaat cggcgcttgt tgcgacacgg ggcattgggt tcgttcggga ttggacccgg
    32341 tggagtgttt gaagatgatg gaagggcgtg tgatcacctt gcatttgaaa gacgtggccg
    32401 aggtcggcgt cgtcgaagcc cgcgatgtcc cgctgggtac cggcgccgcc aagtatgatc
    32461 ttgtattgca agagctcgcc cggcaaaagt tccgtggcgt gctgcccatt gaatacgagc
    32521 acgactcgcc ccagttggaa gaagatgtcg ctgcctgccg ggacttcgtc ctcgggtggg
    32581 ctgcaaaaaa tgtgtcttga tcgaacgata gtgtggttcc tcccgaaagt aaagttttcc
    32641 cgccttgaag gagccgaacg atggcctgcc accgtagccc tgcgcccagc gtattcaagc
    32701 attggttcat cggatttgtg atcggtgtaa gctgcctcct ggccagaggc caaaggccgt
    32761 ttggtgcgat ggagggcttt atcgttttcg cggcggaaga taatccgcag ttttcaaaac
    32821 tttggggacg cgaaggggag ttgtggacac cccagagccg tttgcccgac ttttcttacg
    32881 ccggttaccg gtggggacaa gaatcgtacc gcattcccaa ggaaagcatc tcggtgaagg
    32941 actttggggc caagggcgat ggccaaagcg acgacacggc ggcgttcaaa cgtgcgcttg
    33001 ccgcgggaaa gggcaaggtg attgtcattc ccccggggcg gtatatcttg cgagatattc
    33061 tgacgatcag tagctcaaat accgtcttgc ggggtgccgg gccgggcaag acctttctcg
    33121 tttttaccaa gccggggatc gaaatcgatc cgcgaccttc caagacggac ggcaatcagc
    33181 cgacaacgaa ctggtcctgg gcgggcggat tgatctccat cggcggtcgc cgccaacagc
    33241 cgcgggatgt ggtcaaggtc gttggttcgc aaaaacgggg cgacacacaa ttgagtgttg
    33301 agaaaaaccc cttccgcccg ggtgatgaga tcctcctcac cctgcgagac gatgaaaaga
    33361 attcgctgtt gacttacctt taccgcggcc agccgggcga tatctcgggg ttgcgaaact
    33421 ggtccgtgga gcaggtcttt cggatcgtga gtgtggaagg atcgacgctc aagctgaatc
    33481 gtcccttgcg ttttgatatc cgctccgagt ggcaacccac cgtggagaga ttcctgccgc
    33541 cgctcgccga tgtgggcgtg gaagggatca cctttgattt tccggttcag cagtacgcag
    33601 ggcatttcaa agagattggt ttcaacccag ttgaaatcac ccgctccgct gcccattgct
    33661 ggttaaaaga ccttgtgatc cgcaatgccg ataatgggcc gtttgtctcg ggttacttct
    33721 gcaccgtgga aaagattcgc tttgaagccg atccacagcg ggtatcaagt tcgggaaact
    33781 atacgggtca ccatggcgtg acgtttctgc gttgctccga ttgcttgtgc cgagatttcg
    33841 aattcaacac gcgattcatc catgacctga cggtccaaag ttctattggt tgcgtgtttt
    33901 gttccggccg tggtattgac atctgttttg atcatcaccg ctgggcacct tatgaaaacc
    33961 tcttcacgga catcgatgcg gggacgggaa aacgcctgtt cgcctccagt ggcggcggga
    34021 tgcggggtaa tcacacggcc gggggagaaa ccttctggtg cattcgtacc caggggctgg
    34081 tccgctggcc aaagagcctg ggcatcgatg cgatcaacgt cgtgggactg aacgtcagcg
    34141 atgccgccga agccgtcccc tcgttgccgt ctccgacaga gttaaccggt cgctggtgtg
    34201 agccaatctc gccggaggtc ctgcaaccgc aaaatcttta cgacgcgatg cgcaaaaggg
    34261 tcgcctcgcc gcagtgagca agagataatg ccgcatttgt gcggtgcccc gtcgagcaac
    34321 gccgggcccg gaaagtttac aattctcgtc aaataagttc tggacttact ccagcggcgc
    34381 gctagaattg gcctgtaagt tgcggcgtac tgtggacagc gagatcagat ttgcatcaag
    34441 gacgcagtgt gctgctccca ctttggactc ccggcgtgaa tgtccaggcg tattactttt
    34501 gatggcagta cctgtgttgc ctttcctcaa caccccttgc taacgcgttc gctttgagaa
    34561 acatcctttt ctgatcacac atggctcttc agtgtaagaa ccagcctatg gtgatacgga
    34621 ggaacgcaaa cgaggcatga tcggaccaac cttaaaactt tggcacgaaa cctgcatgca
    34681 aaagcgacga ggttccatcg tgcgtggaga gtatccccgt gttgggccga gatcataacg
    34741 tgaacaagaa ggtaaactga tcagggagct gtcacccatg actcgtcttt tttcgcaacg
    34801 tcgccgacgt ccttcccggc gtggcatcct gagcttcgaa tggattctgc ttatctccat
    34861 tcttgtgatt gggattattg gagggctggc cgccgtacgt aacgcccttc tgagtgagct
    34921 taacgacctc tcacaagccg tcgaagcgat caacgtgtgc ggcacgtgcg atcccgccag
    34981 ttgcccgccg gggaatcccg attgctgcga cgatccgtgg tggtgttgcg gtccatgagc
    35041 gctgagctgt tccaggctgg caccaactgg ccgcggatat ctctcggcaa gcggttgaca
    35101 gttggggatg tcgctacgaa attaggactc ttatggcacc ccgtgcatcc ggagaagaga
    35161 cgtccctgag tccctcgcac caggctactg aaagcaactc accgcgggga tgtgggcgga
    35221 tgttgccgcg cctggcggtg gggtgtttct ttcttctcat cgccttcctc ttccgtgcct
    35281 tctggctgcc ggtggttgct tatcccctca tggcaccggc accgcagcac gagaatgccg
    35341 ttaccctcat ctgggcccgt agttcggatg gaaaaacacc ctccggggac ggtatcgtcg
    35401 agcggacggc atcactcctc aagacccatc ctcatgcccg ggtcattttt acccaggggt
    35461 ggtcaagtcg tttggtcgaa ctcggggtga ttccgccacc gtacgagacc tttcgcagcg
    35521 ccctggcgaa atatcagatc gaatccgagc gggttatcaa cctggaggtg aatggcttcg
    35581 agttctggaa cgaggccaag gccgtggatg ccttcctccg cgagcatcct gaggaacggg
    35641 tcctcctact agtggaggaa tttgccgggc aaagccaccg ctatgtcctc cgcagggccc
    35701 tcgacgtgtc gcatcgcgat cgggtggaga ttctaggatt gccggatttg cagtattcgt
    35761 accatgactg gtggaggtcc cgctccggcg tgaagggcgt gatggtgggt tatctgtttc
    35821 tcatccacac catccttcaa ccccaaccgc tgcgaccgcc ccggaagctc tccctggagg
    35881 actttgaaca atcgatccct gaagcgccag gagggagacc atgaagaggg tttggtggct
    35941 tctcgcttgt ctgatcctaa gcggcctggc catcttgttc cacgggcggt ggcttccatc
    36001 cctggccaag tacctcgacg tgggcgaggc accccgaaaa agtgattaca tcatgattat
    36061 gggcggcggt gtcgagaatc ggccgtttac agcggcggcc cttttcaaaa aaggttgggg
    36121 agagcgcatc ttgatcgccg aacttcgccc cacacccggc ggtgagaacc tcctcccacc
    36181 ggagcatgag gtcacaagtc gcctcctcca gaaattgggt gtgcccgagg accggatcat
    36241 cgtcttgaga ggcaatcacg cggccacgtt tcacgagatt gtcacccttg gcgaacttct
    36301 ggcccgggag ccggaggcga gggtcatgat cgtcaccgac cccgtccata ctcgccggac
    36361 ccgctggagc gtgcaacgca ttctgggcga aagggcatcg cggatcagtt ttgtgtcagc
    36421 cccttctgac ggatataacc ccgacgactg gtggcaaact ggccaggggt tttttatggt
    36481 cacgagtgaa tatctcaaat tgctttttta tgcctttcgc tacgggtggg gactgcacct
    36541 gacggcggca gcggttgcgc tcatcctcct tatcggaatc ctcgcgcgct atttgcggcc
    36601 aatgggccgg gataaagcct cgacaaggga agcggtcccc acctcctgag ctccattcgt
    36661 tctggaacaa taccgcccta ccgttgagcg gcgaaccttc tcggccgtgc cccacgactg
    36721 gttgacctca cggtaaaatc atgtacctcg gttgacagat cccaccctca atatggtggt
    36781 gtggtactac cgcatctggc agccatttgg taagatagga cgcggtgttg ggggtgggtt
    36841 agtccgtcct ccccgcatag accttttgac tgtgttgtgg gtggcggttt caccaatcga
    36901 gttggtgcac tcgagcttgc aaggagtcag ggtaccatga ctgaatcaag tcacactttc
    36961 tcctcagaga agaaaatccg tgttttcatt gcggatgatc acgacatggt ccggataggc
    37021 ctcaaggcca tgttgtctgt gccggacatc gaggtggttg gcgaggctgg tacgggcgag
    37081 gatgcggtca atttcctgct caacaatgat gtcgatgtcg cgattttgga tgtgcggatg
    37141 cctcgcggag acggcctgaa tgccctgggg cgaatcaagc tggaaaaacc cgagttgccg
    37201 gtcctcatgc tttcaaccta cgataatccg acctatatcg cccgggccgt ggccttccaa
    37261 gcccagggtt atcttctcaa gagttgttct cgcgaggaat tactcgatgc catccgcaag
    37321 gcagcccgcg gcgaaaatat ctggacgcgg gacgaactcc gccgtgtggc gggggggctg
    37381 gccaccccac ggatggccgc cgacgtggat attcccctca ctcagcggga aagcgaggtc
    37441 ctcaaacagt tggccaacgg cctgacgaat aaggagattg cccaaatcct caacatcagc
    37501 tacgagactg tcaaggaaca cgtccagcac atcttgcgga agatcggcgt cacccatcgg
    37561 acgcaggcgg cagtttgggc cgtcaaaaac cgtctagtct gaataccccg catcctttgc
    37621 ccgattttgc cccaccattc gggtcgcgcg agaccgatct tctccgcacg ggcggagtct
    37681 gtatgccaag tttctacggg gaacgatggg gtcttctgtc ctccttggcc ccttagagtg
    37741 tgtggaggat ggtcatggtg cagtttcccg tgtcgatcgt cgatttaccc aagaccggtg
    37801 tgctgaccga cgatccgtct ccctgccctc aaatggattc ggccgcgtgc cctcaaatga
    37861 gacatcggcc ggccggtaat gtccaactgc ccccgggtgc agaaacatcc caggtgggtg
    37921 agtctcccga cccaagattg acgctcccag cgccggtctc gcttctagga ttgccgattg
    37981 tcccttggcg ccattatgaa gtgatccacg tcattgattg gctggtgggg cgcggcgagc
    38041 cgtcatattt tgtcacggca aatctacact atgctcggtt gtcggcaacg atcccggccc
    38101 tcaaggctgt gaatgaccac gcagctttta tcgtggcgga cggcatgccg ctggtgtggc
    38161 tctcgcggtg gcggcgaaag cccctaccgg agcgaatcac cggtgccgac ctgatctggt
    38221 caatgtgtga actggcggcc aacaggggat accgcgtttt tcttttgggt ggggcaccgg
    38281 gggtggccga aacggccggg cagcgccttc aggcacggtt gcgcggtctt cagattgccg
    38341 gaattgccgc cccatgtttg gatacactaa cccctcttga gcgaactcgc ctgatcaccc
    38401 aaatccgaca agcgagaccc gatattctct tcgccgcttt tggtcaacca aagggtgaac
    38461 tgtggctagc cgagcacatt caggaattgg gtgttcccgt cgcggttcaa atcggggccg
    38521 ccctcgactt tgtggcaggt cgggtccgtc gtgctccgcg gtttgtgcaa cgaatggggc
    38581 tggaatgggc ctatcgtaca ctccaagagc cccgccgatt gctaccgcgc tatctggccg
    38641 acgggtggtt ttttctctgc cagttgatgc gttcttcgcc gcgttcctga cacgactctc
    38701 ttttacgacc tcggctcccg agagatgatt tggcttatga tcctccaccc acgcgatgct
    38761 tgagcacgat ggaatctccg ccgtcatcag cccactgcac ggggacaagc caacccactg
    38821 ttccaatgcg acaaagtccc cggccagact tttcgcacca aaaataggta cacacaatcc
    38881 gcagggactt gacgttactg ttcaaacccg tgagagacac ctccagcgga ccttcccctg
    38941 gcgggatgcg aacgacctgc ttatcaccgg ctgaatcaac gccttggtca ctgagccgtt
    39001 cgatgacaag atcggtaggc acattcggat tgaggccaaa gcccgcgaga aactcaaaga
    39061 ggatctgaag ctttatcgtg cccccggggc gaagtctaac gaccggctct tggcgaagat
    39121 cgaccttgtc ctcggggaat gtgccttttt cttcgacggc gggcggcggc tccaggccct
    39181 tgagcgcgag cgttgtgact tttagggtgg gctgaagctc gatcacacga atggcgtggt
    39241 tgtttgtgtc ggccacaaaa aggcgatccc tagcgaaggt gattccagcc ggttcgtcga
    39301 accgcggtgg gtcgttggta tcaccggctt gcctatcacc caccaaggtc atggtggttc
    39361 ccgtgcgggg atcgatccgc ttgatcttgt tgttgtaagt gtcggccaca tacagaaatc
    39421 cgttgccgta acaaacgcca atcggatgtt gcaggaggac ctgatcgggc ggcccatcgc
    39481 gatccccgaa ggtgaacaaa cgagcggcga gaagtcgcga tgtgccaaca atggtgcgaa
    39541 cctccccgcg gggctgtaaa ggcacagccc gaatcgaact gccctcactg tccgccacgt
    39601 aaagccactc gccatcggtc gccagcccac tcggctgggc aaacgaggca aatcccagcg
    39661 catacgccga gggcggcaag agcggaccat cgacgatatc ttcgcgggca ttaccggcat
    39721 agggaccgat ccgcgtcaag gcagtgtcca tccgccaaat ttggtggcaa ccggccatgg
    39781 cgatatagat ccaatcgccg accacacaca ggtcccacgg gctgctcaat tcggcctgca
    39841 agggtggccc catccgcatg accggaggac gacgtcgctg cacgcctgtc cccgccaccg
    39901 tgcgaaccgt tcgcttggtg aagtcgatct cacggatggc gtgattctcg gtgtcggcca
    39961 cgagaagccg atccccaagg atgaccatcc cctgaggtga gcaaaacctc gcttcactcc
    40021 atgcgccgtc tcgcaaacct ggaatgccgt caccaatgac atcaacaacc tggccgtcaa
    40081 ggcgacacac cacaattcga tggtgcccac tgtcggcaat gaaaagtcgc tctcctgcct
    40141 cgtcggccaa aacctttccg ggaaaccgca agggagtccg tgcggctcgc gaggattccg
    40201 tgacaaaatg gacaggcgaa cggtcaagga gtcctctctt gtcgtagaac gccacggcag
    40261 gggccaggat ggcgtccagg gcctcgaagg tcgtttcacc actctgggcc caaacaacaa
    40321 acccctcggg gtcgatgagg accaaggtcg gccaggcgtt gaccccaaac cgtcgccaaa
    40381 caagcatgtc ggagtcgtta atcaccgggt gttcaatgcc atatcgcaag atcgcctgga
    40441 cgatgttctg ggattctttt tcctcctgga atttggcgga atggacccct acgacgacta
    40501 actctcgcgg atatttggct tcgagtttct tcaactcggg aatgatatgc atgcagttga
    40561 tacagcagta ggtccagaag tcaaggagca cgaatttccc tcgcaaatcc cgccaggtca
    40621 acggcccggt ggtgttcagc cattccaagt tctcgggcaa gtctgggaca ggacgacgca
    40681 tagcaaaagg ggcttgttcc acgcaaaggg cctccagggg ggaaaggagt atcgcaccgc
    40741 caagaatcat cactgcaccc aaaagacggc atctcgcaat ggacgcccga tcccgacgac
    40801 agattgcggc gtcgttctcg cggaataccg atcgacttat caagcgaacc atagacgtta
    40861 ccctttttcg gctatcagcg cggtggactg ggacgccaat cacttgaagc ggccacgaac
    40921 aagcgtagcc ctcaatcggc gaaggtcacc acgacggcgc gtggtattcc ctatcaatgt
    40981 ctcgaggcac acctgaccgt tcatgacaaa accacgctgt gatgaatcgt attcgccagg
    41041 gaccctttgt ggcaaggacg ctgtggcgat tgggaaacac accgaataca ccgcgttacc
    41101 cagagcctat cccacaagtt cccctctccc gttcggcgag aaagggatga gagtggctcg
    41161 aatcgctgcc ggttttctcg atggactccg acgttcgctt gccctcaccc caaccctctc
    41221 ccagaaggag agggaacttt gggataggat cttaagagga taaaggccag ccatcaaaat
    41281 ccggcgaatt aggaggcctg gggtaactgc gagacaagct tgaccagctc ttggtgaagc
    41341 cgctgggaaa tgttatggcc gccgaccccg cgggccgtct ccgccgcaac gacttgctgc
    41401 tgggccagtt
//
LOCUS       DAOSUT010000010        40164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_696_length_40164_cov_9.793961, whole genome shotgun sequence.
ACCESSION   DAOSUT010000010 DAOSUT010000000
VERSION     DAOSUT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 40164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40164
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_40164_cov_9.793961"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1082)
                     /locus_tag="PLD05_01720"
     CDS             complement(156..1082)
                     /locus_tag="PLD05_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOP76174.1"
                     /translation="MVSQPFTVGYVELHRSGELQRRIEEALARLADCTLCPRQCHANR
                     LEDDPVGSYCGIGRHAWVSSAFPHHGEEPCLRGWNGSGTIFFSGCNLKCVFCQNNDIS
                     WQRTGYPADARRLAHLMIHLQEQGCHNINFVTPSHVIPHILEALPIAIEKGLRVPLVY
                     NSSGYDSVEALRLLDGVIDIYMPDMKFTNPDVAKRLAHAPDYWDVCRAAVKEMHRQVG
                     DLVLDDRGLAVRGLLVRHLVMPNNCAGTHEVMSFLAEQISPHTFVNIMAQYRPEGRAW
                     DIPEIARRCTREEYLEAIAVARKAGLHRFCRD"
     gene            1531..2538
                     /locus_tag="PLD05_01725"
     CDS             1531..2538
                     /locus_tag="PLD05_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76175.1"
                     /translation="MRIKLPRAFTLVELLVVIAIIGLLIALLLPAVQAAREAARRAQC
                     LNHLKQLGLATHNYATSWGCFPPSAIVDRSVQSTGNNVSWGIHGRLLAYLEQVPLGSR
                     INLVEPWDQQPAIDSVRIAVLACPSDPKAGQLRDPGGGKPRLYSTTYGFNLGVYFVYD
                     PVSDRGGDGISYPNSSLSWAAVTDGTAHTILAAEVKAWQPYRRNGGPPTAEMPESPED
                     ASAIVQSATDAKTTGHTEWPDGRVHHTGFTACFPPNTRIPCEIQGQMLDCDYNSWQEG
                     RDGIRGLPTYAIVVSRSYHPGGVNAVHVDGSARFYSQTIEKAMWQALATRAGGEILAS
                     P"
     gene            complement(2643..3416)
                     /locus_tag="PLD05_01730"
     CDS             complement(2643..3416)
                     /locus_tag="PLD05_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76176.1"
                     /translation="MAFTRSVDPQQIGLTAAAAGSLSSHEDSSLSEREANGLPPPVIN
                     VRNLEFSFDGQVRLFSHLDFTLSPGEKVGLLGPNGSGKTTFLHILMGLIPAYAGTIEI
                     LGRVRRHPSDFRDLPGQVGLLFQDSDDQLFCPTVAEDVAFGPFNQGKSPREVREIVRQ
                     TLDKLGLAGWESRITYRLSGGEKRLVALATVLAMEPQLLLLDEPTAGLDESACARVEK
                     ILSALPQSMVIVSHDRGFLQRICHRVLVLKDGRLSLCHA"
     gene            complement(3446..4216)
                     /gene="cbiQ"
                     /locus_tag="PLD05_01735"
     CDS             complement(3446..4216)
                     /gene="cbiQ"
                     /locus_tag="PLD05_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOP76177.1"
                     /translation="MNRQWDEGPMLRHSSAAPIDRFDPRLRTLAVGLTALGTVAAGRL
                     LTLLGLLALGLVFLALARMPLAVTIKRCLPLLGFGVLLAMILPWTVPGTPLGTGFWAH
                     ASSEGLTQAGLITLRALIIILATIGLVGTLEIPVLGHAFVHLRIPPKLVFVFLYAVRY
                     LQVLQDEYHRLRTAMVTRGFRPAMNRHTYRAYGYLVGMLLVRSLERSERILTAMRCRG
                     FRGKFYVLDHFHFTSWDVLFGLICVIATGLYFGWEVVW"
     gene            complement(4269..4937)
                     /locus_tag="PLD05_01740"
     CDS             complement(4269..4937)
                     /locus_tag="PLD05_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76178.1"
                     /translation="MRSSRITVILVGFVAANLATAAAFAHSLYLFAAADGEKINGRVY
                     LRGGTGVPDVIVKVYAEAKEAVGQVTTDSEGGFSFSAPYRCDYLLRAVMEDGHQAEAL
                     IHADELPDTLPPFPGQQTPTAVTRPLESQTAGQTPASGVLPLNHTAEVSQSTSAAQST
                     ATNDESVRQEVASLRSQVIQLREDLKQLQTTTGLRDIFGGVGYILGLTGITFYFLAKR
                     GRNK"
     gene            complement(5052..5696)
                     /gene="cbiM"
                     /locus_tag="PLD05_01745"
     CDS             complement(5052..5696)
                     /gene="cbiM"
                     /locus_tag="PLD05_01745"
                     /inference="COORDINATES: protein motif:HMM:NF004905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOP76179.1"
                     /translation="MHIQDGVLTASTAGTVLLALGWVGAGLGTTLGLSRLDADQVPRT
                     GLLTSLFFVVSLIQVPVGPISVHLVLNGLLGLILGWTAFPAVLIALLLQWLFFGMGGL
                     TTLGINTVNMALPAVLARYAFGRLVCSPRVQLVALGGFAVGAVAVLLAATMTALSVAV
                     AGREFVVVSWIVWIGDGVIAVGEGIVTAAAVVFLRRVYPQVFGAAILEGRVPAW"
     gene            complement(5763..6644)
                     /locus_tag="PLD05_01750"
     CDS             complement(5763..6644)
                     /locus_tag="PLD05_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOP76180.1"
                     /translation="MLKTRQAERWALRTGIFSRIARCGREAVIRRSTLAIFLAIAAPV
                     QAHFLVLLPSQDVVDTPEQATLVFDIQFTHPMEQGPVMAMGTPSRFFVSHGGQQIDLI
                     TLLQPRSLQGQPAFVARYRLNSPGDYVFAIEPAPYWEKMEKKWIVHYTKVVVNFMGDE
                     GDWAKPVGLPVEIQPLTRPYGLWTGNCFRGVVLHNGQPVPFATVEVEYWNTDKRVAVP
                     NDAFVTQVIKADGQGVFCYGIPRAGWWGFAALVDAAEKRPAPGSEPADVELGGVIWVR
                     AADMVETPSQSSQNTHK"
     gene            7093..8223
                     /locus_tag="PLD05_01755"
     CDS             7093..8223
                     /locus_tag="PLD05_01755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76181.1"
                     /translation="MTTTNTTRTLDGVGLTRTWGGAVGRERLQVLCDTYNRLLNQRRR
                     LNRTEHLWLIRELGRGGQGVVYLSERRGADGFTLPLALKFFAPDPYPNDAAYEEAMAR
                     IGWVSGRVSRIAHDNLLDVDNWLDCDGIRVMVMEWVDGYDLARLLTKRMYQLLEQRVK
                     PIRWQYINEVIVTAGPVQPRLKPGVAVAIVRDCLAGLAALHRGRIVHGDIKPSNIMLK
                     RTGTAKLVDVGSAVDLDNPPPMRGCTPLYAAPEVLESDGAEISPRSDLASLGYVLIEM
                     LSGQPPFPANLPRAQLLEAKRLLPQRLHTILPPECVASEALMNFCKMLIAPDPARRFS
                     NAESADLDNNGAAAFLRQLVKGDLASEYATEIRHWLSDLHGL"
     gene            8259..8786
                     /locus_tag="PLD05_01760"
     CDS             8259..8786
                     /locus_tag="PLD05_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOP76182.1"
                     /translation="MMKRTLVLGLGNDILTDDAVGLRVARELKSILAGRPEITVDEDC
                     WGGLRLMERLAGFDRAIIIDAIQSGGQPGTVRILSPKDIPTQHSASAHDVNLPTALAF
                     GRKMGVHLPEDHEIVLVAIEALDILTFGERCTPAVEAAIPQAVRLVLDLLAGWDNSLP
                     DRNSGLGFGQSTREL"
     gene            8888..9331
                     /locus_tag="PLD05_01765"
     CDS             8888..9331
                     /locus_tag="PLD05_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOP76183.1"
                     /translation="MVSPEMLRRYSYFAKVSEETLKEIAMLAEEKSYPAGMRLFYVGD
                     PADHLNIIVEGEVDIQYELGNGEKRTVDTLVPGDILVWSALVEPYRTTAIGTTTKPTK
                     VIAIKAKPLRELCDKDSMLGYQLMREIAKLLASRLESARVQLAAT"
     gene            9367..9741
                     /gene="hypA"
                     /locus_tag="PLD05_01770"
     CDS             9367..9741
                     /gene="hypA"
                     /locus_tag="PLD05_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOP76184.1"
                     /translation="MHEVALVESLLESVHEQLREAGIVGRVRRLDVIIGPLSGVVPEA
                     FRFAFKVLSPESLGPGCELVIHTRPARCQCQSCGAVSEIAELTYVCPVCGSHDIRLEG
                     GRELMLQSIEVDENDPCGDPPA"
     gene            10071..10721
                     /gene="hypB"
                     /locus_tag="PLD05_01775"
     CDS             10071..10721
                     /gene="hypB"
                     /locus_tag="PLD05_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOP76185.1"
                     /translation="MKIVAAKKILKANDQLAEENRHRFHAAGVFVINVLGSPGGGKTT
                     LIEALHGRLPIKRGLAVIEGDLAGTIDADRLAARGIAAVQIATEGICHLDANMVSAAS
                     RELELEKYDWLFIENVGNLVCPAGFDLGEDLRLVLLSVPEGDDKVIKYPTVFQSSQAV
                     IITKADLLPHFEFQVERVRQDLRKLNPNAPVFCTSAKTGEGIEEIATWLANAATGR"
     gene            10920..11561
                     /gene="rsmD"
                     /locus_tag="PLD05_01780"
     CDS             10920..11561
                     /gene="rsmD"
                     /locus_tag="PLD05_01780"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOP76186.1"
                     /translation="MARRRGKAEDEGQPKRRTPVGLRIIGGRLRGRRLLYDGSWSIRP
                     MRDRVREAIFNILRDAVVDAHVIDIFAGTGAMGLEALSRGAKFVQFIERDPTACRVLL
                     QNIERLQLQEQTEVIVSDAFIWWKHCGPLENVPHVVFCCPPYELYQTRGHEMLQLITG
                     LVRSLPNDSVLVVESGEDVDVHQFPYPDKWDVRLYRPSRIGFFWKTSSSDEAA"
     gene            complement(11603..12049)
                     /locus_tag="PLD05_01785"
     CDS             complement(11603..12049)
                     /locus_tag="PLD05_01785"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOP76187.1"
                     /translation="MADRLKILFLCTGNSCRSQMAEGWARHLKGDQIEAHSAGIQPQG
                     LNPRAVAVMAEAGVDISGHTSKPLAEVMSIPFDYVITVCDDAHEACPVFPGKAQVVHV
                     GFPDPPRLTRHLQDEEAILQVYRQVRDAIRDFVQGLPDNLCNLARK"
     gene            complement(12138..13145)
                     /locus_tag="PLD05_01790"
     CDS             complement(12138..13145)
                     /locus_tag="PLD05_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOP76188.1"
                     /translation="MSDFYPMGRDPVQRPPQWPYDGGSFQAEHVGYEFDSERDQPMRP
                     STAPPEVVMAELAGPPPRSRARFVVPLVLLILTIISTFVAAGGLGNPWDGLKYSVCVM
                     AILLSHEMGHFLQALRYRAPATLPFFLPMPFSPVGTFGAVIAMDTRRLDRRALFDIGA
                     TGPLAGLVPTIVFTVVGLQQSQVVSAARIPGVMELGEPLLFTWLTRLIHGPLPEGYTV
                     MLGPMAFAGWVGLLITALNLIPIGQLDGGHILYALLGRKANTVGMFLLLGAIVAVVIA
                     GYWGWLIMIFLLGLLGPEHPPTANDRAPLGALRIILGWLMLAFIIVGFTPEPFKLFPQ
                     S"
     gene            complement(13500..15044)
                     /locus_tag="PLD05_01795"
     CDS             complement(13500..15044)
                     /locus_tag="PLD05_01795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOP76189.1"
                     /translation="MLVWVVFITVATINLILGIAVAVWMKRGIAGHQSDDLSNNLELF
                     SGIIPSPPSTTVSQQSTNSDDEAADRDAPPNDSISPDITASDSATYAESAAANSTTEA
                     FVVDRLGSTQESDSSESPVNVNSAEIASDKTRMPESEDQGPVEPASEESPPQSGPQED
                     DVPSETDAEAAVEAPSSEEGEIPGLSRLLELCSKKVHELIDIFEWMRSQPPTKGEELN
                     TILARVPPIMASFFDEYGQIVPKLVSTEHGGPIPEETLACLEEHALAAEQTVQRLTML
                     DYRGHLQATLQQALAEFGRLIARLQRTRDELEAAFAQGLINFQPHKEVDEKIQRDALT
                     GLFNRTGVEVFFEKHWGGEPHKRKPLWAAILDLDQFRQINQEHGYSLGDELLKAVAQL
                     LAEVVPQGAFLARYRGDQFLVVRTDCQSKRFIHTIEEVRQSVEVTKFRYAGQTFSLTV
                     SCGVTESRTDDVLTSFCERLDEALLEAKRAGGNRTFIHNGEFARPIIPLNLKVAEREV
                     SVATVG"
     gene            complement(15175..16863)
                     /locus_tag="PLD05_01800"
     CDS             complement(15175..16863)
                     /locus_tag="PLD05_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOP76190.1"
                     /translation="METRQGWQELVERLKARRNVEPITPRPLRVYREEVPIPPDCDLA
                     TLPGWRALCRSFLQTTGCLLRFLPGAQPSSPDDIPVMPPTVDASGPVGVRSPLGHLRI
                     DPTTRAPKGLNPDHPIYRMARGMAEVLGELLATRIALWQREAELAAGIPVIPHKEEEQ
                     HLAQRLDEILHSATRAVDAQAAAIYILDETTSHLKLRAIWGLPKTKLLAPPRNLKSAM
                     ADLEAMLGHAVIVDRSQPVDHWNPPEEFATSVCLPISTPTTILGTLWVFSNQQRTFSD
                     RDIDLLEVAAGRVAAELEREAALQAALQTAQLQRQVERAQRLIRSQLPSVSPLVDGWE
                     LAGWMDTERMGGAFFDWCCPKDGRIHFALGEAAEDSVSTALTMAAVKAAWRAHAQYQR
                     ETKKLLEQLNLTLWTSSAGDQSAIMVCGIVDPKTGTLWLAAAGTAYAMLITEKQHRVL
                     PPGGANLGVDPESTYLPVRWKFPENAILVATTAGVRSAVGVDQRPLWETQILPLLTSN
                     LHLPAKILVQHVQRAIEQNAVTPNKVPAVLIIKRRPSPKHPETDSRLRENSQHA"
     gene            17455..18699
                     /locus_tag="PLD05_01805"
     CDS             17455..18699
                     /locus_tag="PLD05_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76191.1"
                     /translation="MNRQVRFEIVVWVSIAVLSVCVSGHLQAGQPSPNAGANWGLEAS
                     SLDTSDQWPAGTWDRAASHGDPVPVQGTAPCCEICGAGYDCPRLWTWYNGAKILNRGR
                     ARSALLTVNPAGEGLLNVDNANFDVAGGYETRVQYFLKNDPENRDQFIEFVYWGLNEW
                     KDQQSVNGQRLTYQSSLFGGTSFEAGSLFTFYDFVDFNYADLHRIRYESEINNFELNW
                     WFKPRPRPDRLALLPNGKWQRQCQPGVYFNYAFGFRGLSLDEGFIFDSRGVVDAQGGP
                     ETLYGRQTIRSKNDTFGLQLAGELLFRRHRFEWGARVKAAPMFNWAEQTTDIALAHNP
                     GHVVRYAKDDDVCFMGEVSIVANYRIFPRMRAFASWDMMWLDGVALAPEQLDFGLKSP
                     GVINDNGTVYFQGVSLGFDVVW"
     gene            18876..20240
                     /locus_tag="PLD05_01810"
     CDS             18876..20240
                     /locus_tag="PLD05_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOP76192.1"
                     /translation="METRGQIGRLRTMGHGNVLVLLLLTAVFRAVVLLWPGAFTTDPD
                     SYQRVAQNLASRGIFALNGEPTAYRPPLYPVVLSGIMCAVGPYWRIGLGIIHWLFGIG
                     TVLCVIYLVRRRSCRVCALAVLFVIADPILLHHSRLIMTETQSVFLVAVTLALFHAGT
                     SRAERIWWFFGGVFLGLCSLTRPTFLPWPILAVLLWLLGSVQLSRDTSDMTRPSVSDV
                     DSLPPKAHGRRLAGVGRIGLAAIGFAVAIVPWGVRNWLILGRPIIGTTHGGYTFYLAN
                     NPWYYKYLRQPGVKPVWDATEFNAQWVKTLEAKQLHDELAADRLAYELAFETIRHQLT
                     MFLRACYDRFCQLWRLIPNRLDQDEPLVLWGLRHGVGLFYLVEYVLVGLGILGWLKRR
                     DFQTPDRVLAVLVCALVLTVVGIHLFYWTNMRMRAPLIPMLALLAGEGYHYLTSRRLE
                     TEQT"
     gene            complement(20262..22328)
                     /locus_tag="PLD05_01815"
     CDS             complement(20262..22328)
                     /locus_tag="PLD05_01815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76193.1"
                     /translation="MNEPQQLGPYRIVRRLGRGGMGTVYEAVHVVTNEPAAVKTLSES
                     LIEEPDFRARFELEIETLRKLNHPNIVRLIGFGEEQGILFYVMELVHGPSLDQELRAG
                     RKFHWQEVAEIGVQICRALRHAHDRGIIHRDLKPANLLRAENGTIKLSDFGIARLFGA
                     PSHTGVGSILGTIEYMAPEQAESLPIGPRADLYSLGAVLYALLVGRPPFKVTSFAEIL
                     HLHRTSQPAPVRSFVPDCPEELDRIIRELLAKDPDKRIANALLAGRRLQAMLHALTKH
                     KVAGSEDVLHATPGHLIDFGVEQGDRRSVPVDGEAPTAALPSSPEPPRPSLGETITAA
                     GSPSESPENQAAAFPVGTPSMWKPTRETDVFRYLEKESPVPDHPEDGLGSTATAEPKE
                     QKGRTEQPSPPVTSSATQTTRKFVEVPPEELDKIEPSPSERQPIFSLQTGILLAALVV
                     LAITIWYFLQPPTADQLYNRVMAKVGDGTDSEALLSAAPDIEAFLTRYPEDARAPSIR
                     EYQQEIDLLRLERRFELQSRQKASAHSSIPIERDYLQALRYAQLDPELGIEKLEALLA
                     LYDYAPTEKLPADKSRETPQGKSRRPVGHTAECLELARRRLEQLKKQLAPAITNQRDL
                     IAGRLEEANQLEATDPEKADAIRRAIIVLYGDKGWAKPYIEQAEAALQRRSQKSLPSQ
                     ISTTSP"
     gene            complement(22678..23385)
                     /locus_tag="PLD05_01820"
     CDS             complement(22678..23385)
                     /locus_tag="PLD05_01820"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76194.1"
                     /translation="MFRNMLAWCWRLSLVLIVSIGAEVLAQESANESKHPDSSQWPEL
                     FARDLSNAIFPAGVWAFQDGVLTATEDQCIWTKDQYANFILDLEFKNSEGTNSGVIVY
                     CSDIKNWIPNSVEIQIADDFAEKWAKAPATWHCGAIFGHLAPKKSVVKKPGEWNRMTI
                     TCRGPMIQVVLNGELVTEMDMRKWTSAKTNPDGSEIPAWLSRPKAELPQKGHIGLQGK
                     HGDSPIYFRNVRIKVLE"
     gene            23730..25004
                     /gene="rlmN"
                     /locus_tag="PLD05_01825"
     CDS             23730..25004
                     /gene="rlmN"
                     /locus_tag="PLD05_01825"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOP76195.1"
                     /translation="MEHLLDYSWKQFRDWFVSKGQPGYRVAQVLHWVYESPKISFDEM
                     SDVPKVVRRELKKSFKIFSMQVASHRKGSDGTEKLLLRTQDGEFVECVLLRDDKGNRT
                     VCISTQIGCGVGCAFCASGLDGVVRNLTSGEILEQILQLKCLLQPEERLSHIVVMGMG
                     EPLANLGRLLGALHRATHKDGLGISARHVTISTVGLPNGIRRLAELNPQYHLAVSLHA
                     PNDELRNRLVPANRRIGIQAILQAADDYFAKTGRRVTYEYVLLRNVNDRPEHAQELAE
                     LLRGRNALVNLIPYNPVQGLPYEVPDRRDVGRFADILSRMGIQVTIRYRKGSEIEAAC
                     GQLRRVHPEIVSKSKKKKKKKSAAAGQEPTGSSESSPTSDNTSTSDSVIISNDATVAI
                     SDHELQMYEATEDLPAEAEQAAPAKPSDQVSE"
     gene            complement(24996..26012)
                     /gene="ruvB"
                     /locus_tag="PLD05_01830"
     CDS             complement(24996..26012)
                     /gene="ruvB"
                     /locus_tag="PLD05_01830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOP76196.1"
                     /translation="MTREPILKPSVSTDEDERLLRPQRMEEMVGQRDVYERLQIAIDA
                     ALKRDEPLGHILFDGPPGLGKTTFALCIPRHLGVSIQIASGATLKAPKDLIPYLTNAE
                     ERSVLFIDEIHRMQKSVEEFLYPAMEDFRIDITLGEGVNARTLNIRLKPFTLIGATTR
                     SGLLSAPLRDRFHMREHLDFYTVDELAEIVRRNAQKLQIKIDPNAAEEIARRSRGTPR
                     IANNRLRWVRDYATSRANGRITLQVAEDALAMQGVDKLGLDVQDRKYLETIARVFGGG
                     PVGVEAVAHTMNLSPDTLIDEVEPFLLRSELVVRTPRGRKLTPKAYEHLGMSYPTPNQ
                     GYLF"
     gene            complement(26346..26816)
                     /locus_tag="PLD05_01835"
     CDS             complement(26346..26816)
                     /locus_tag="PLD05_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76197.1"
                     /translation="MTKTRNVFSVTCLALVGLALLFFALPGCGGKRSVPGRPKTVPVK
                     VTVLYRGAPVAEANVQFLPEGSGNAATGVTDANGIARLTTFDQNDGAVPGKYRVTIRK
                     SIQVGGSNPADPDAPPVPATYREELPAKYAAPETSGLTAEVSESQKEFTFELTD"
     gene            complement(27001..28056)
                     /locus_tag="PLD05_01840"
     CDS             complement(27001..28056)
                     /locus_tag="PLD05_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76198.1"
                     /translation="MTVCVGRCGRKPRWGFTLVELLVVIAIIGILIALLLPAVQAARE
                     AARRSQCTNNLKQLALAMQNYHDVYTRFPARQAGSGHQNTGSNDTHRGRLSGWVAVCP
                     YMEQQSLYDQINALPHQAPWNNTSWYNVNIPALNCPSDPASTPPTGTARGTVTYCMNG
                     GDSPIDSFNNPPSSTSIRGVFHVFQWSSMAEISDGTSNTALISERLRPRAQNSLGNVV
                     GLSLGTNPLACRAAYDASQKKYITGSFISDTPPGFRWADGAAWFAGFTTILPPNSASC
                     ANAADHWERGIYTPSSRHPGGVNVAFADGSVRFVSETVDTGNLGTDFPSVTSGGMSPY
                     GVWGALGTKSGGETASL"
     gene            28705..30303
                     /locus_tag="PLD05_01845"
     CDS             28705..30303
                     /locus_tag="PLD05_01845"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOP76199.1"
                     /translation="MFEEFLQAARSIFEQAQHEGRLIRNPDTARLRAMALEEPEARET
                     CYGNVAVESEPTSRAAMFTKNNIDTRFGKEEYDLLEKAKRELSKKELVSIDVQVGDGT
                     EGVTARLIVPRDFAHVAFAGLKLFKPTSTDEPTYQVIMFTDEAFESNKKKKLPDKKKK
                     LPDKDITIRLAHAPDGRMVKFVRNSNYFGEWKKGVFAGEDWRVKQKGDAIFLHVGCRR
                     DTLETPHGGYRTVCSLFAALSANGKTSTTCKVLARKGRERSWLIQDDGGALHRDGSFR
                     GFEPGGIFIKTDGLNPGDQLEAYYACLKRETFLENVWIEPDGTIDFFNMERTSNGRAV
                     VERRDFMHAAQSINSERVDNLFLITRGSTIPAIAKLTHEEAAAFMVLGQSMESSAGDP
                     TQAGKIKNCFFYDPFLAGDRAEHANLFYDILKANPHIQCYLLNTGYVGEGESMRDIRL
                     ADTMGILDSVLRGGLDEWVYSPRTKLYVPSAVRLVDSILFHPEKLFPHDVFEEKQKAL
                     EKHRAEFLDRFPGLHPNIRKVFQR"
     gene            30434..33241
                     /locus_tag="PLD05_01850"
     CDS             30434..33241
                     /locus_tag="PLD05_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76200.1"
                     /translation="MKTLLLMVALVWGTLFTVGWAVAQMPKQPPNVIILITDDQGFGD
                     LSCHGNPVLKTPHLDRLHDESIRFTDFHVAPMCTPTRGQLMTGRDALRNGAMNVSSGR
                     TPLRREFPTMAEVFAASGYSTGLFGKWHLGDNYPYRPQDRGFQESIWYPSSHIGSVPD
                     AWENDYFDDIYFHNGRPRQFRGYTTDVFFTEAIRWMEEQAKQGRPFFCYLATAAPHAP
                     HFVPEQYRNAIRQSLEAARPQLSKFAAGLQPEARSSFEEELVRFLAMIANIDENVGRL
                     EGFLKSSGLRENTLLIFLTDNGSTFGPRYFNAGMKGGKTTLWEGGHRVPCFIRWPGGG
                     LRPAGDVPGLTQVQDIFPTLVELLQLRLPAGVQFDGISLAPVLRGQSEVPEDRMLVIN
                     YSRMPFKSTRTTADNNAVPRREGAAVLWKRWRLLEDKALYNLAHDPLQERNVIDQYPE
                     VAAKMRSHLNAWWDGVKARVNEFQAVIVGDDAENPTMLTACEWADVFLDQQAQVRRGE
                     RKSGLWHIEVAKPGEYAFMLSRWPYASGLRLRDGISETKVTDGILPAGEAWPVTAARI
                     RVGHMERVIRPVGDAIAARFVIPLDVGRTTLETMFLDENGQEIAGAYYVTVERLRQVE
                     QPAAVILDTDMSGDCDDAGALALLHALADRGECKILATIVNRKDKTNASAAAVDAINT
                     YYGRGNLPIGTDKRGPTDLQRTSAYAPALRDEFPSDIGPDDQAPDAVDIYRQILVSQP
                     DGSVTICSVGALSNLAELWRSEPELVRKKVRRLVIMGGEFPTSKTPETNIRTHREAAK
                     IVAEEWPGEIIWVGFEIGHIVFTGEGLKLTPRHNPVRRAYELRPFGGRPAIEGGQPSY
                     DQTAALIAVRGLEPDLWEVVTGGRVDVDAEGRTTWRVDPTGKHSYVRLACPPAKLATI
                     IENLMMAPPVAHMAEENDPRQ"
     gene            33533..35056
                     /locus_tag="PLD05_01855"
     CDS             33533..35056
                     /locus_tag="PLD05_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOP76201.1"
                     /translation="MHRARRRTLMKDTRKVVNSLATVGLVIIAALIPLAVVWAESASA
                     ASSEGGKVFKAGAAASNITPWLDEGIVGGWSSPPGKYIHDELYARCLVLDDGSTQLAI
                     VIVDNLGLAREVCDRAKQSIREHTGLPEDHVLIAATHTHSSISARPPNVLKPEEDLNQ
                     YGQFVARRISDGVRRAMAHLEPARIAWGTVDVPEHVNNRRWFMKPEIKLVNPFGGLDK
                     VRMNPPASSPDLLEPAGPVDPQVCFISIQSRDGRPIALLANYSLHYVGGVGPGHISAD
                     YFGVFADRIQELLKADRLEPPFVGILSNGASGDVNNISFQKPRPAKKPYEQMRYVAYD
                     LAQKVFEAHQDLQWQDWVPLGMLQQELVLKVRKPTPEQLKRAKEILAGAPPLPERPHE
                     ETYARRLVQLAESPDTVSVPLQALRIGEVGITAIPFEVFAEIGLEIKEKSPFRPTFTI
                     SHANGSYAYLPTPRQHELGGYETWLGTCKVEEQASVKITETVLGMLRALRGESTNNP"
     gene            complement(35088..37067)
                     /locus_tag="PLD05_01860"
     CDS             complement(35088..37067)
                     /locus_tag="PLD05_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOP76202.1"
                     /translation="MLRLLWTSQGVSHRLGSRAWVWVVVAATVCFAFSSLCQAEESHL
                     LAYWSFDESDQRVAHDMSGNDFHLLLQSARRGSGISGQALVLKGSDSVMLAEAFDEFF
                     VLSDDYAVEAWVKHDQKTPQIYLSKWTGTGMGSAWWMGFFEGCVQWAEYNDQFQVRLK
                     GPDIADGRWHQVVGVRSGKQLSLFVDGELITQRVTPFEPAGHNTAPLKIGGFGAGRRS
                     SWPLVGSIDEVRIYCRALSAQEINERFKLISSGQQGPVLQPIANGLPARTIIRLDVPS
                     LIEAGKSRSFSLVLVSNRPFSQDTLTARFSIKDNGGRLLKEWQLPISPKAGSRVSNHP
                     VELPALPEGTYDLEAAIGSQGASGKIQVKNLEPTVAYNLRIKNERAKKSAFYRGIVSA
                     YSGMRYKPDGTPDVEAMLGHLSHLGVNVYTYLIAPRSSQELAALGEFCQKARSAGIEV
                     WVYLVPPSEAPIDREKPIRERRYPPFDMDYVRWAEAVAQVSLDHPNLTLWMIDDFDGN
                     LGFFTREYTQQIYKTSKQINPRLLFGVCVYHESLANFVKAGYLDYVDALLWGYQHSSA
                     RYPECGLDATTLPLEIHDYLQTGKIAIPCIYFTPHSSWTEGRPTKAYLEEAMKTAFEQ
                     AGIVWVFTTPRPGTMQEEVVRAFTQRHPLPKWPGQ"
     gene            complement(37147..39960)
                     /locus_tag="PLD05_01865"
     CDS             complement(37147..39960)
                     /locus_tag="PLD05_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76203.1"
                     /translation="MPGDLSAFGGEITFENELIRYKIAENGQNSSFFDKKGNREQLIL
                     AQPGYFTTIRQAGRTFVPQKCIRDNDTLTVQFEQARVVLQTRIRPKHLTFEVTSVEGE
                     VDELRLFNLLVRPGQLISNIAGLAQDDRFAVAVRSLNLLILGQLSGRSSSGLTAPADS
                     EPMPSQLAFVTAVAPKERGLVGAAVALVGCPVGEVRTVLQEVLETEDAVRSRLGGPWA
                     QDAPETQGSYVFATVSEENADAWIALAKQAGIAQIHMISWEQSLGHYQPRKNLFPNGI
                     EGLKQVVEKIHAAGLKAGMHTLTGCISPSDPWVTPKPDPRLAVDVRFTLVDPLDEKAD
                     RIVTREKPGNLDTVWAYGGHGNVLRIGDELIQYSGLSQEPPYGFTGCRRGAFGTQPQK
                     HEAEAPVEHLFVRYGCFLPDENSTLVEEIAQAIADVYNTCGFDMIYMDGAEGMPGGWY
                     GISRMREEIFRRLKRPALVEASCWDYHSWAFHSRLGAWDHPNWGLKPFVDWHCRSNEQ
                     HRRSSLLPVQLGWWAILGPTADHDAELSDEFEYLCAKSLAYGMPMSFQGVAPGDNPPC
                     ARQPEYLEMLGRYEHLRLSNQVPPQIRERLKQEGQDFHLEIRDDGSWAFRPADYLSHK
                     ITGAVPESTTWTVNNRYSAQPLRVRIQALYGVEPYDSANAEEIVSFKEGEFSQVAQAA
                     GVKAELSYVTDEIKVGSSSGKLVAENTRGTSRGAWAHFTKTFDPHVNMQKRAAMGVWI
                     HGDGSGALLNLQLTNPLHYWPTCDEHYVRLDFTGWRYVEFLLRERDADDFHRYEWPYG
                     SYYAVVYRSPLIRDHVSAMNLFINEVPANGKTTCLISPIRTLPVVSIKIGQPSLELNG
                     QRLIFPVGLTSGGYLEFDGQNPARVYDERGKLTQTVDVEGTVPVLNAGNNTIHFTCQP
                     EVPPGVHPRVKVTVITMGDPLSP"
BASE COUNT         8787 a        10940 c        11642 g         8795 t
ORIGIN      
        1 agggttgggg tgagggcaag cgaacgtcgg aatcgattga gaaaaccggc agcgattcga
       61 accatcctca cctctgagcc ctgtcccgcc gaacacgtga ggggagatta tgggatagtc
      121 tcttcgtctc cggtaaatga ttttctggga aagttttaat cgcggcaaaa acggtgcaag
      181 ccggcttttc tggccaccgc tattgcctca aggtattcct cgcgtgtgca acgccgagca
      241 atttcgggga tgtcccacgc acggccctct ggtcgatatt gtgccataat gttgacaaat
      301 gtatgagggg agatctgctc ggccagaaag gacattactt cgtgggttcc agcgcagtta
      361 ttgggcatga ccagatgccg caccagcagt ccgcggacgg ctaacccccg atcgtccagg
      421 accaaatcgc ccacctgtcg atgcatttcc ttcacggctg cgcgacacac gtcccagtaa
      481 tcgggtgcat gcgccaagcg ttttgcgaca tcgggattgg taaacttcat gtccggcatg
      541 tagatgtcga taaccccatc tagcagacga agggcctcca ccgaatcgta cccgctgctg
      601 ttatagacca aaggcacacg gagacccttt tcaatggcaa tcggtagggc ctccaaaatg
      661 tgcggaatga catggctcgg cgtgacaaaa ttgatgttat gacagccttg ctcttgcagg
      721 tgaatcataa gatgggcaag gcgacgcgca tcagcggggt agcccgttcg ctgccagctt
      781 atgtcgttat tttgacagaa gacacacttc aaattgcatc ccgaaaagaa gatcgttccc
      841 gatccgttcc agccgcgaag gcagggttct tcgccgtggt ggggaaatgc gcttgatacc
      901 caggcgtgcc gaccaatgcc gcaataagac cccacaggat cgtcctctag gcgattggca
      961 tggcattgtc gtgggcacag cgtgcagtcg gccaatcggg ccaacgcctc ctcgattcgc
     1021 cgctgcaatt caccggaacg atgaagctcc acataaccga ctgtaaacgg ctgggatacc
     1081 ataagaagcg tttacggtat agcatcaggg gtcacgtatt tgccttccat gctcacttca
     1141 tctggcgcag gaggcaaaaa acaattggaa cgccagaata gttacccact ccttattttc
     1201 cgtaagcgcc ggtcatcaca aaagacaggt gtgccacttg ataggtgtgg cggcgagaac
     1261 ttttcgacta gcctttccgc aggataaacc atgatgcgcg attcgacatc gtgtgaaaca
     1321 ttgtgacatg cactctcggc tgaacggttc ccgacacggc gtctttggtg gcaaaagacg
     1381 cggttttgtg aactctcggc cagaacgaac ccccttggga gcgcgatctg ctgtgctcaa
     1441 ttgattcatc tcgacggtaa gtggcatggc ggggaaagag gtcgcgctcc cggagcgtag
     1501 tgcattagca gttcggaaag gttgaaggaa atgcgtatca aactaccaag agcgtttacc
     1561 ctggtagaac ttctggttgt gatcgctatt attggcttgc tcatcgcctt gttacttccg
     1621 gcagtacagg cagcccgaga ggcagcccgg cgggcgcagt gccttaacca ccttaagcag
     1681 ttggggctag cgacacacaa ttacgcaact tcctggggat gctttccccc aagtgctatt
     1741 gttgatcgga gcgtccagtc aaccgggaat aatgtttcct ggggtatcca tggtcgtctt
     1801 ctggcctacc ttgaacaggt gccgttgggt tctcgaatca acctggtgga accttgggac
     1861 caacagcctg cgattgactc cgtacggatt gccgttttgg cttgtcctag tgatcccaag
     1921 gccggtcaac tccgcgatcc aggcggtggc aaaccacgac tctattccac cacctatgga
     1981 ttcaatttgg gtgtgtattt cgtttatgat cccgtttcgg atcgtggtgg ggacggcatt
     2041 tcctatccaa actctagcct ttcctgggcg gcggttaccg acggaacggc gcacaccatc
     2101 cttgcggctg aggtcaaggc ttggcagcct tatcgccgga atggcggtcc cccaaccgca
     2161 gagatgcccg aatctcccga ggatgcgtcg gcaattgtgc aatccgctac cgatgccaaa
     2221 acgactggcc acactgaatg gcccgatggt cgtgtgcatc acaccggctt tacggcttgt
     2281 tttcctccca acaccagaat cccctgcgag atccagggcc agatgctcga ttgtgattac
     2341 aactcttggc aagaagggag ggacggtatc aggggactac ccacctatgc catcgtggtt
     2401 tcccgcagtt accatcctgg tggggtgaat gcggttcatg ttgacggctc tgcccggttc
     2461 tattcccaaa ccatcgaaaa agcgatgtgg caggctttag cgacccgggc cggtggcgaa
     2521 atacttgcct cgccatgaag tctgcattag atgcgcaatt aagatgcccc caacggttca
     2581 aatgctggct tgtgccagtc ccacttcgaa tatcttagct gtgcgttgtc cacttatgcc
     2641 cattatgcgt ggcacaggct gaggcgacca tccttcaaca caaggacccg gtggcagatt
     2701 ctctgcaaga acccgcggtc atgagacaca atcaccatgg attgtggcaa cgcggaaagg
     2761 attttttcca cccgcgcgca agccgattcg tctagaccgg ccgttggttc atccaggagc
     2821 agcagttggg gctccatggc gagaaccgtt gccagggcaa ccaatcgctt ttcgccgccg
     2881 gataatcggt aggtaatccg cgactcccat ccggccagcc caagtttgtc taacgtttga
     2941 cgcacaatct cccgaacctc gcgtgggctt ttcccctgat tgaaggggcc aaaggcgaca
     3001 tcctctgcca ccgtggggca aaacagttgg tcatcactat cctggaaaag cagtcccacc
     3061 tgtcccggca gatcacgaaa atcgctagga tgacgacgta cccgccccag aatctcgatt
     3121 gtgccggcat atgccgggat aagccccatg aggatgtgca agaatgtcgt cttgcccgat
     3181 ccattgggcc cgagtagccc cactttttcg ccaggagata atgtgaaatc gagatgagag
     3241 aacagccgca cttggccgtc gaagctaaac tccaaattac ggacgttaat gacgggcggc
     3301 ggcaggccgt tggcttctcg ctccgaaaga gaagaatctt cgtgactaga caatgaacca
     3361 gctgcggcag ccgtcagacc tatctgttgc ggatcgacgg agcgggtgaa tgccatcttg
     3421 tcccctttgg gttggggttg ttctgttacc acacgacttc ccatccaaaa taaaggccgg
     3481 tagcgatcac gcagatcaaa ccgaacaaaa catcccagga ggtaaaatga aagtggtcta
     3541 ggacgtagaa cttccctcga aagccgcggc accgcatggc ggtcaaaatc cgttccgagc
     3601 gttcgaggct tcttaccaag agcatcccca ccagataacc gtaggcccga taggtgtgtc
     3661 ggttcatggc cgggcgaaag ccccgcgtta ccatggccgt tcgtaaccga tgatactcat
     3721 cctgaaggac ttggagataa cggacagcat agagaaacac gaacaccagt tttggcggga
     3781 tgcgcagatg aacgaaggca tggcccagaa cagggatttc cagcgtccca acaagaccga
     3841 tcgtggcaag aatgataatt aatgcccgaa gcgtgatcag ccctgcctgc gttagtccct
     3901 ccgaacttgc gtgggcccaa aaacctgtgc ccagcggtgt gccgggaacg gtccagggaa
     3961 ggatcatcgc cagaagcaca ccaaatccca ggagcggcaa gcatcgcttg atagtcacgg
     4021 ccagcggcat tcgcgcaagg gccagaaaga ccaaacccag cgcaagcagc ccaaggagag
     4081 ttagtaggcg tcctgccgcc accgtcccca aggcggttag gcccacggcc aacgtccgca
     4141 ggcggggatc gaagcgatct atgggggccg cgctgctgtg tcgcagcata gggccttcat
     4201 cccattggcg attcatatcg aggatttttc tcccattgca tcaaacgatc tcacgagtta
     4261 atgatttgtc acttgtttcg ccctcttttt gccaggaaat agaaagtaat acccgtcagc
     4321 ccaagaatgt aacccacgcc cccaaaaata tcgcgaaggc cggtggtagt ctgcaactgc
     4381 ttcaaatcct cacgcagttg gatgacctgg ctacgcaggc tggccacctc ctggcgaacg
     4441 gactcatcat tagtggcggt ggattgagcg gcactcgtag attggctgac ctcagccgtg
     4501 tggttgagcg gtaaaacccc ggacgcgggc gtctgccctg cggtctgtga ttcaaggggc
     4561 ctcgttacgg ccgttggggt ttgctggccc ggaaaaggag gcagcgtgtc ggggagttca
     4621 tcagcatgga tcagggcctc ggcctgatgg ccgtcttcca tcacggctcg gagaagataa
     4681 tcgcaccgat agggggccga aaaggagaat cctccttcgc tatcggtcgt gacttggccg
     4741 acagcttcct tggcctcggc atagactttc acaatcacgt ctgggactcc cgtcccacca
     4801 cggagataca cccggccgtt gattttctcc ccgtccgcag cggcaaaaag atatagggaa
     4861 tgggcaaatg cggcggcagt cgctagattt gccgctacga aaccgaccaa gataactgtg
     4921 atccgggatg atctcatctt cttacacctg cttatcgatt cccctttgcc taatagcgat
     4981 cggcggagga atcagtacgc gtgtccagga acacgtctga taatgctttc acccttgcac
     5041 tgaattgtgg cttaccaagc tggaacacgg ccctccaaga tggcagcacc aaacacctgg
     5101 ggataaaccc gtcgcaaaaa gacaacggcc gcggctgtga caatcccttc accaacggcg
     5161 atcactccat cgccgatcca aacgatccag gaaacgacga cgaattcgcg gccggcaact
     5221 gccacggaaa gggctgtcat cgtcgctgcc agcaaaacgg ccacggcccc cacagcaaat
     5281 ccccccaggg cgaccaattg tactcgggga gaacaaacca atcggccaaa ggcgtagcgg
     5341 gccaacacgg ccggtaaggc catgttcaca gtgttgatgc ccagtgtggt taaccctccc
     5401 attccaaaaa acagccattg gaggagcagg gcaatgagga cggctggaaa cgctgtccaa
     5461 cccaagatca accccaataa accgttgagc accaagtgaa cactgatcgg accgacggga
     5521 acctgaatca gggagaccac aaaaaagaga cttgtcagaa gtcctgtgcg gggcacctga
     5581 tctgcatcca agcgtgataa ccccagcgtg gtccccaagc ccgcgccgac ccaacccagg
     5641 gccaggagga ccgtgcctgc cgtggaggct gtgaggacac catcttgaat gtgcattgac
     5701 gttaatcttg ccttgaggca accagtggtc ttaaaggtga aatgtggaac aaaggtgaca
     5761 gatcatttgt gcgtattctg gctggattgt gacggtgtct ccaccatatc ggctgcccga
     5821 acccaaatca cgccgcccaa ttctacatcg gcgggttcac taccgggcgc cggacgtttt
     5881 tcggcagcat ccacgagtgc cgcaaaaccc caccacccag cccgcggtat cccgtaacaa
     5941 aaaacacctt gtccatcggc cttgataacc tgcgtgacaa aggcgtcgtt gggcacggcg
     6001 accctcttgt cggtattcca gtattcaacc tcgaccgtgg caaacggcac gggttggccg
     6061 ttgtgaagga cgacgccgcg gaaacaattg cctgtccaca ggccataagg gcgcgtcagg
     6121 ggttgaattt cgaccggcaa acccacgggt tttgcccaat ctccttcgtc tcccataaag
     6181 ttgaccacga ctttggtgta gtggacaatc catttcttct ccatcttttc ccaatagggt
     6241 gccggctcga tggcaaacac gtaatcgccg ggactgttca gtcgatagcg ggcgacaaag
     6301 gcaggctggc cttgaagcga ccggggctgc aatagggtga tcagatcgat ctgctgaccc
     6361 ccatggctta caaaaaaacg ggaaggcgtt cccatggcca tcacgggtcc ttgttccatg
     6421 ggatgagtaa actggatgtc aaacacgagg gtggcctgct cgggcgtatc caccacatcc
     6481 tgggagggga gcaggacaag aaaatgggcc tgcaccggtg cggcgattgc caggaagatg
     6541 gccagggtgc tccgcctaat gacggcttcc ctgccacacc gggcaatcct cgaaaagatg
     6601 ccagtccgta atgcccatcg ttcggcttga cgcgtcttga gcatagaaac aaaggcccca
     6661 acaaacgatg gcgaattcct gaaaatggac acaaggtcag tgttaccaat tctgttattg
     6721 tagcactcaa tgaaaaggga agtccatgac ctgaaaaaga ttgagagcaa tgaatcgtct
     6781 ggcgtaagac cggcgacgtt cttatggcga ccacgaagac ggcgggacgc tttcgcgcgg
     6841 cggctgtcaa ctggcaagcg ttcggacaaa gagcgaaccg aaacgcattt tgcccgtgtc
     6901 ttgtggcaag ggctgatcag ccggccgtaa cagcggagta gtcaaattga tgtcacacac
     6961 ataggtggag caagacccgc ccccatggcg aaatgtgtat tgcaatcttt acgatgaggc
     7021 aaggtttttc gtatgattgt gataacacaa ggaggtgtct gacttgatca ttgttcatga
     7081 gctttgatat acatgaccac gaccaatacg acgagaaccc ttgacggggt tggattgaca
     7141 cggacctggg gtggtgcagt tggccgagaa cgactgcagg tcctttgtga cacatacaac
     7201 cgccttctca atcaacgtcg tcgcctaaat cgcaccgagc atctatggct gatccgcgag
     7261 ctgggccgcg gaggacaagg ggttgtctat ctaagcgagc gacggggcgc ggacggtttt
     7321 acgctgcccc tcgcgttaaa gttctttgcc cctgatccct atcctaatga tgccgcgtac
     7381 gaagaggcca tggccaggat cggttgggtt tctggccgtg tttcgcgtat cgctcacgat
     7441 aatctcctcg acgtggacaa ctggttggac tgcgacggta tccgcgtgat ggtcatggaa
     7501 tgggtcgatg gctatgattt agcccgcctc ttgaccaaac ggatgtacca acttctggaa
     7561 caacgtgtga aaccgatccg ctggcaatat atcaacgagg tgatcgttac ggcgggcccg
     7621 gtgcagccac ggctcaaacc cggcgtggcc gtcgccattg tgagggactg tttggcgggg
     7681 cttgccgcgc tccatcgagg gcgaatcgta catggtgata tcaagccttc gaacatcatg
     7741 ctcaagcgaa ccgggacggc caaattggtt gatgtgggtt cggctgtgga tttagacaat
     7801 ccgccaccga tgcgcggatg cacgccctta tatgcggccc ctgaggtctt ggagagtgat
     7861 ggcgccgaga tttcgccccg ctcggatttg gcgagtttgg gttacgtctt gatcgagatg
     7921 ttgtcgggcc agccgccgtt tcctgccaat ttgccacggg ctcaactttt ggaggcgaag
     7981 cgtcttttac cgcagagatt acacaccatt ttaccgccag agtgtgtggc cagtgaggcc
     8041 ctgatgaact tctgcaagat gttgatcgca ccggacccgg cccggcggtt ctccaatgcc
     8101 gagtcggccg atttggacaa taatggcgca gcggccttct tacgacaact tgtgaagggt
     8161 gacctggctt ccgaatacgc cacggaaatt cggcattggc tttctgattt gcatggcctg
     8221 taatctggtc gcccccttgc cgtgatcttc gcctaacaat gatgaaacgc accttggtcc
     8281 tcggattggg aaacgatatc ctcacggatg acgccgtggg gctgcgtgtg gcgagggaat
     8341 tgaaatcgat cttggccggg cgtcctgaga ttaccgtgga tgaagattgc tggggcggcc
     8401 tgcgattgat ggagcggctg gctgggtttg atcgggcgat tatcatcgat gcgattcaat
     8461 caggcgggca gccggggaca gtccgaattc tctcgccaaa agacatccct acccaacaca
     8521 gtgcttctgc ccacgacgta aacttgccga cagccttggc ctttgggcga aaaatggggg
     8581 tacacctccc agaagatcat gagattgtcc ttgtcgccat tgaagcactg gatatactga
     8641 cttttgggga acggtgcact cccgcggtgg aggcagccat tccccaggcg gtgcggcttg
     8701 tgttggatct cctcgccggg tgggacaact cattgcctga ccgcaattcg ggtctcgggt
     8761 ttggacagag cacgcgggaa ctgtgactgt tgcttggcca ggatcagcgc cctaaagcga
     8821 caggctgttg ggatcatggg tgcgataggc cctgctaacg gtcaccaaga ctgggaggag
     8881 tttgaccatg gtttcgcccg aaatgttgcg tcgttattcg tatttcgcca aagttagcga
     8941 ggagacgctc aaggaaatcg ccatgctggc ggaagaaaag tcgtatcctg cggggatgcg
     9001 gctattttac gtcggtgatc ccgcggacca tctcaacatt attgttgagg gagaagtgga
     9061 catccaatac gagctgggta acggggagaa acgaaccgtc gatacgctcg tgcctggaga
     9121 catcctcgtc tggtcagcgt tggttgaacc gtaccgaacg accgcgattg ggacgacaac
     9181 aaaaccgacg aaggtcatcg ccatcaaagc caagccctta cgggagttgt gcgacaagga
     9241 ctcgatgctc ggttaccagt tgatgcgaga aatcgcgaaa ctcttggcca gtcgtcttga
     9301 gtccgcgcgg gtccaactgg ccgccacctg aagcgtggct cttagtgtaa gaattcgcaa
     9361 accgacatgc atgaagtcgc gttggttgag tccctcctgg agtctgtgca cgagcaactt
     9421 cgggaggcgg ggattgtcgg gcgagtccga cggttagatg ttattattgg gcccctgtcg
     9481 ggtgttgtgc cggaggcctt tcgctttgcc tttaaagttc tttcgcccga gagtctcgga
     9541 cccggctgcg agttggtaat ccacactcga ccggctcgat gccagtgcca atcatgtggg
     9601 gcggtctcgg agattgcgga gctgacctac gtttgcccgg tctgcggcag tcatgatata
     9661 cgcttggaag gtgggcgtga gctgatgctc caatccattg aagtggatga aaatgaccct
     9721 tgcggtgacc caccggctta gagcctatcc ccaaaaagtc cccctctccc tcagggagag
     9781 ggttagggtg agggcaagcg atcgtcgcaa tcgattgagc aaaccggcag cgattcgagc
     9841 caccctcacc cctgacccct ctcccgctga acgggcgagg gaaaattatg ggatgggttc
     9901 ttgatcgccc tcgcgggagc cggacgactc ataggcgcac gctcggctga actgtcaacg
     9961 aatttgctgc gtaaaatcaa gaacaggaga gaggggactc atggcgataa aattgctcgc
    10021 gagcatcttt ctcgcatcgt tgcgtttgtc ttcctattga gctttgttct atgaagatcg
    10081 ttgcagcgaa gaagatcctt aaggccaacg accaattggc ggaagaaaac cggcatcgct
    10141 tccatgccgc aggggtgttt gtgatcaatg ttttgggctc gcctgggggt ggaaaaacga
    10201 cgctgatcga ggccctccat gggcggcttc ccatcaaacg tggcctggcg gtgatcgagg
    10261 gcgatctcgc aggcactatt gacgccgacc ggttggcagc gcggggcatt gcagccgttc
    10321 aaattgccac cgaagggatt tgccacctgg atgccaacat ggtctcggcg gcgagccgag
    10381 aactggaatt ggagaagtac gattggcttt tcatcgagaa cgttggcaat ctcgtttgtc
    10441 cggccggatt tgacctcggc gaggacctgc ggttggtctt gctgagcgtt ccggaaggag
    10501 atgacaaggt catcaagtat cccaccgtgt ttcagtcttc gcaggcggtg atcatcacca
    10561 aggctgactt gttgccccat tttgaatttc aagtcgaacg cgttcggcag gacttacgaa
    10621 aattgaaccc caacgcacct gtgttttgca cctcggccaa gacgggagag ggcatcgagg
    10681 aaatcgccac gtggctggct aacgcagcga cggggcgata ggccgcggtt cttgcgatcg
    10741 gacgggacat taggccacgt gttgtgggat gatctcgcgc gatatcggaa gaaaatctgt
    10801 tctcctcccg cgctattgct gcgcttggga gaaatggaaa gcgatgttac aatgcaacgt
    10861 ggtccgagcc gtggttcata agaccttttc caggcacggg atatgattga tagctaaaca
    10921 tggccaggcg tcgaggaaaa gcggaagacg aaggtcagcc caagcggcgg acaccggttg
    10981 gcttgcggat catcggtggc cgacttcgcg gaaggcgtct tctctacgat ggttcctggt
    11041 cgattcgccc aatgcgcgat cgggtgaggg aggcaatttt caacatcttg cgggatgccg
    11101 tggtcgatgc ccacgtcatc gacatctttg ccgggaccgg ggccatggga ttggaggccc
    11161 tgagtcgcgg ggccaaattc gtgcagttta tcgagcgcga tccaacggcg tgccgcgtgc
    11221 tgctgcagaa catcgaacgg cttcagttac aggagcagac agaagttatt gtcagcgatg
    11281 ccttcatttg gtggaaacac tgcggcccat tggagaatgt gcctcatgtc gttttctgtt
    11341 gtccaccgta cgagctatat cagactcgtg ggcacgaaat gcttcagctc ataacggggc
    11401 tagttcggtc cctccccaac gatagcgttc tcgttgtgga atctggtgaa gatgttgatg
    11461 ttcaccagtt tccttatcct gacaaatggg atgtgcggct ctatcgccct agccggattg
    11521 gctttttttg gaagacttca tcgagcgacg aagccgcctg atcgtcgcgt cgaagaccga
    11581 acgatgttgc accgcccccg tgctatttcc gtgctaggtt gcaaagattg tccggcagtc
    11641 cctgaacaaa atcgcggatg gcatcgcgaa cctgacgata gacttggagg atcgcttcct
    11701 catcttgcag atgccgggtt aaacggggtg gatcaggaaa gcccacatga accacctgag
    11761 ccttgccagg aaagacgggg caggcctcgt gggcgtcatc gcacacagtg atcacgtaat
    11821 cgaaggggat ggacatgacc tcggcaaggg gcttactggt gtggccggag atgtcgaccc
    11881 cggcttccgc catcacggcc actgcccgtg gattaagccc ttggggctgg atacccgccg
    11941 aatgcgcctc gatttgatca cctttaaggt ggcgtgccca gccttcggcc atctggcttc
    12001 ggcacgagtt tccggtgcat agaaagagga tctttaacct atcggccatg gctagtgctt
    12061 ttctagctcg aagtgaccaa tctccccgac gggcctttct tttcgctcgt cggtggggag
    12121 ccttcatcaa gacaattcta cgactgaggg aagagtttga acggctcagg ggtgaatccc
    12181 acgatgatga aggcgagcat cagccagccc aagatgatcc ggagcgcccc gagtggtgca
    12241 cgatcgtttg cggtcggggg atgttccggg ccgagcagtc ccaacaagaa tatcatgatc
    12301 aaccaacccc aataaccagc gataaccact gctacgatcg ccccgagcaa taaaaacatt
    12361 cccacggtgt tggcctttcg accaagtagg gcgtaaagga tatggccacc atccaactga
    12421 ccaatgggaa taaggttgag cgccgtaata agcaagccga cccaaccggc aaaggccatc
    12481 ggcccaagca tgacggtata accctcgggc agtggtccgt gaataagacg agttaaccaa
    12541 gtgaaaagga gcggctcgcc caactccatg acaccgggaa tcctggcagc cgagacgacc
    12601 tgggactgtt ggagaccgac gacggtgaag acaattgtgg gaaccaggcc agctaatgga
    12661 ccggtggcgc caatgtcaaa aagggctcgg cggtcgagac ggcgtgtatc catggcgatc
    12721 acggccccga acgttcccac gggagaaaat ggcatcggga ggaaaaaggg taatgtggcc
    12781 ggcgcacggt agcggagcgc ttgcaaaaaa tgacccattt cgtgggataa gaggatggcc
    12841 ataacgcaaa ccgagtattt caggccgtcc cacgggtttc ctaacccgcc ggctgccacg
    12901 aacgtgctaa tgatcgtcag gatcaacaaa acaagcggga caacaaagcg tgctcttgaa
    12961 cgaggcggtg gcccggcaag ctcggccatc accacttccg gtggggcggt gctgggccgc
    13021 atcggctgat cccgttcgga atcgaattca tagccgacat gctctgcctg gaacgatccg
    13081 ccgtcgtagg gccattgagg tggtcgttgg acggggtcac gccccatcgg ataaaaatcg
    13141 ctcataccta tgcccaccaa tcaagtgtaa ggaattgagt ttacaagact gtgcaagtct
    13201 cacacacagg agcccatccc aaaaagtccc cctctcccgg agggagaggg ttggagtgag
    13261 ggcaagcgaa cgtcggaatc gattgaaaaa aacagcagcg attcgagcca cgctcacccc
    13321 cgacccctct cccgccgaat gggcaaggga agattacggg ataggcttta gctgcccatc
    13381 ttatcctaag tgtacaggat ttctaggttt tgagtatggc ttctacgaag acagttaccg
    13441 caagaaagcc ctgtggtcgt ctaaagacgt ttcaaacgca tgtttgagag cgcctggaat
    13501 tatccgactg tggcgacgga cacttcccgt tctgcgactt tgagattgag cggtatgatc
    13561 ggtcgggcga actcgccgtt gtggatgaag gttcggttac cccccgcccg tttggcctct
    13621 agcagggctt cgtccaatcg ctcgcaaaag gaggtaagga catcatctgt tcgagattct
    13681 gtcactccgc agcttacggt gagggagaat gtctggccag cgtagcggaa cttggtgacc
    13741 tccaccgatt gacgcacttc ctcaatggta tggatgaagc gtttcgactg gcaatcggtt
    13801 cggacaacga ggaactgatc gccgcggtat cgtgccaaaa aggccccctg ggggacaacc
    13861 tcggcaagaa gctgggcgac ggcttttagg agctcgtcac cgagtgagta cccatgttcc
    13921 tggttgattt gccgaaattg atcgaggtcg agaatcgcag cccacagtgg cttgcgtttg
    13981 tggggctcac ctccccagtg tttttcgaag aagacttcaa cccctgtccg attgaacaat
    14041 ccggtcaggg catcgcgttg aatcttttca tcgacctcct tatgcggctg aaagttgata
    14101 agcccctggg cgaaggccgc ttccagttcg tcgcgcgtcc gctggagccg cgcgatcaac
    14161 cgcccgaatt cggcaagggc ttgctggagc gtggcttgga ggtgaccacg ataatcgagc
    14221 atggtcagcc gttgtacggt ttgctcggcg gcgagggcat gctcctctag acaggctaaa
    14281 gtttcctcgg ggattggccc tccgtgttcg gttgagacta gcttgggcac gatctggcca
    14341 tactcatcaa aaaatgatgc catgatggga ggcacccggg ccaggattgt gttaagctcc
    14401 tcgcctttgg ttgggggttg cgaacgcatc cactcgaaga tatcgatcaa ctcgtggacc
    14461 ttcttggagc ataactcaag aaggcgactc aggcctggga tttcgccctc ttccgaggag
    14521 ggggcctcga cggcggcttc cgcgtctgtc tcgctcggaa cgtcgtcttc ctgcgggccg
    14581 gactgcggtg ggctttcctc ggatgccggc tcgacagggc cctgatcttc ggattcgggc
    14641 atcctagttt tgtcgctggc tatctcggca gaattgacgt ttaccgggga ctcggaggaa
    14701 tcggattctt gagtactacc caaacgatca accacaaaag cctccgtggt gctattcgcc
    14761 gccgcagact ccgcataggt ggcagaatcc gacgctgtta tgtcaggtga aatagagtcg
    14821 ttaggtgggg cgtcccggtc cgcggcttca tcgtcggaat tggtggattg ctgggacacc
    14881 gtagtgctcg gcggtgaggg tatgatgcct gagaatagct ccagattgtt ggaaaggtca
    14941 tcactctggt gcccggcgat ccctcgcttc atccatacag cgaccgcgat ccccaggatc
    15001 agattgattg tggcgacggt gataaagacc acccacacca acatgaggcg ctgccatttt
    15061 caatattcaa taatcctcgg gaatctccat taccaacaag tcggaaaagc cctttctatg
    15121 ggccacaagt cggaaaagcc cccgacaagg gctatatgtc ggcagaaacg ttaatcaagc
    15181 atgttgagaa ttctccctga ggcgtgaatc cgtttcgggg tgttttgggc taggtcgcct
    15241 cttgataatg agcacggccg gcaccttgtt tggtgtaaca gcgttctgct cgatcgcccg
    15301 ttgcacgtgc tgtaccagga tcttggccgg taggtgaaga ttcgatgtca gcaaggggag
    15361 gatttgggtc tcccacaatg gacgctggtc caccccaaca gcggatcgga ctcctgcggt
    15421 cgttgccacg aggatggcgt tctcggggaa tttccaacgc acaggtagat aggttgattc
    15481 gggatcaacg cccaaattgg cgcccccggg tggcaggacg cgatgttgtt tttcggtgat
    15541 taacatggcg taggcggtcc ctgctgcggc tagccataac gtacctgtct tcggatcaac
    15601 gatgccgcag accatgatgg ccgactgatc accggcggaa ctggtccaga gcgtcaaatt
    15661 gagttgttcc aggagtttct tggtttcccg ctgatattgg gcgtgtgccc gccaagcggc
    15721 ctttaccgct gccatggtca gggccgtgga gaccgaatct tctgcagcct cgccaagggc
    15781 aaaatggatg cggccgtctt tgggacaaca ccagtcgaaa aaggcgccgc ccattcgctc
    15841 ggtatccatc cacccggcca gctcccagcc gtccacgagg ggtgatacac tgggaagctg
    15901 gctgcgaata agccgctgcg cacgttcgac ttgtctttgg agttgtgccg tttgcaaggc
    15961 ggcttggagg gcggcctcgc gttccagttc ggcagcgacc cgacctgctg cgacttccag
    16021 gaggtcgatg tcgcggtccg agaacgtccg ctgctgattg ctgaacaccc aaagtgtgcc
    16081 aaggatcgtt gtgggcgtgg aaatgggcaa acagacggaa gtcgcgaact cttccggggg
    16141 gttccagtga tcaactggtt gcgagcggtc aacaatgacc gcgtgaccga gcatggcctc
    16201 caggtctgcc atagccgact ttaagttccg cggaggtgcc agcagcttgg tcttcggcaa
    16261 accccagatg gcccgcagct tgagatgact ggtcgtttca tccaggatgt agatggccgc
    16321 ggcctgagca tcgactgcgc gcgtggccga gtggaggatt tcgtccaacc gttgtgcgag
    16381 gtgctgttct tcctctttat gggggatgac tggaatcccg gctgccagtt ccgcctctcg
    16441 ttgccaaagg gcgattcggg ttgccagcaa ttcgccaagc acctcggcca taccgcgggc
    16501 catgcggtaa atggggtgat ccgggttgag tcccttgggg gcccttgtcg taggatcgat
    16561 ccgcagatga cccaagggcg accgtacccc aaccgggcca gaagcgtcaa cagttggggg
    16621 catcacgggg atgtcgtccg ggctgctcgg ttgagccccc ggtaaaaagc ggaggaggca
    16681 gccggtcgtc tggagaaatg accggcagag cgcccgccaa cccgggagtg tggcgaggtc
    16741 acaatcaggt gggatcggga cctcttctcg gtaaactcgt aaaggcctcg gcgtgatcgg
    16801 ctccacgttt cgtctcgctt tcaaacgctc aaccagctct tgccatcctt ggcgagtttc
    16861 catggcttgt cctctcgttg aaaccgttcc gcgaaggata ctcagacgaa ttttagaatt
    16921 ttttcttgtc ttcgcaggca acgcatctct cgcagagcgc gcatatgact tcacatgccg
    16981 gaaagccgcc ataccgcggc atctatatga cttctcgcat gctctgcaaa cattgaagat
    17041 tgtgtagatt gtcctctttg acatcgacat tgagatgtgc ggacttaaaa actgggattg
    17101 atgtgcagct tatgatgact aggaaaaatg cggcggctcg ttgtcttgta ggtgatgagg
    17161 gtaccacacc gatctactaa cctgcgtagc tagacgaaga taaggcaaat ggaggggcgg
    17221 gatagacaac agaggttggt ctgaggcttt ctcatccacc gggcgggcat gctgctgcga
    17281 ggctggaacg ccgaatgttt cccaatttgc cggatatgac gtctgggaag aatttgtcga
    17341 ttatttctgt ttttccggcc tcctttagcc gatgacccat ttgcagcgtc tctacacttt
    17401 ctcaaccttg ggtccctctc tggagtcttt gcaccgagaa acggagggaa acgcgtgaac
    17461 cgccaagttc gtttcgaaat cgtcgtctgg gtgtccattg cggttttgtc cgtgtgtgtg
    17521 agcggtcatt tgcaagccgg gcagcctagc cccaacgcgg gggcgaattg gggcttggaa
    17581 gcatcttcgc tcgacacgtc ggaccaatgg ccggcgggga cctgggatcg ggctgcctcc
    17641 cacggtgacc cggtgccggt ccagggtacg gcgccttgtt gcgagatttg tggtgccgga
    17701 tacgattgcc caagactttg gacctggtac aacggcgcca agatcctaaa tcggggacgt
    17761 gcccgatcgg cgttgctcac ggttaacccg gcaggagagg gtctccttaa cgtggataac
    17821 gccaattttg atgtggccgg cgggtacgag acccgcgtcc agtatttcct aaaaaacgac
    17881 ccagagaacc gcgatcagtt catcgagttc gtctattggg gactgaacga atggaaggat
    17941 cagcaatcgg ttaacggcca gcggctcacc tatcagtcga gcctcttcgg cgggaccagc
    18001 ttcgaggccg gtagtttgtt cacgttttat gactttgtgg acttcaatta cgctgatctc
    18061 caccggattc gttacgagtc cgaaatcaac aactttgagt tgaattggtg gttcaaaccc
    18121 cggccgcggc ctgatcggtt ggccctgtta cccaatggaa aatggcagcg tcaatgtcag
    18181 ccgggcgtgt acttcaatta tgcctttggt ttccgcggcc tatcgctgga tgagggattc
    18241 atcttcgata gtcgaggtgt cgttgacgcg cagggtggcc ccgaaaccct ttacggccgt
    18301 cagacgatcc gttctaagaa tgataccttt ggtctgcaat tggccggcga gctattgttc
    18361 cggcgtcatc gattcgaatg gggtgcccga gtcaaggcgg cgcccatgtt caattgggcc
    18421 gagcaaacca ccgacatcgc ccttgcccac aaccctggcc atgttgttcg ctacgccaaa
    18481 gacgatgacg tgtgttttat gggcgaggta agcattgtgg caaattatcg aatctttcct
    18541 cggatgcggg catttgccag ttgggatatg atgtggttgg atggtgtggc tttggcgccc
    18601 gagcaactcg atttcggcct gaaaagccct ggcgtgatca acgacaacgg gacggtttac
    18661 ttccagggtg tctcgctggg gtttgacgtg gtgtggtagt caaaagtcag gagaccgtgg
    18721 taaatttgct cgggtggggt aggttgagat ccccatcacg ctatgtcggc ttgcctttgg
    18781 cagcatgtgc gcacaaggcc acaacgagcc accggttccg gtcttgtacg taatgaccgc
    18841 cagcgcggat ggttgagaac agtcgggacg gcctcatgga aacgcgcggt caaattgggc
    18901 ggttaaggac catggggcac ggtaatgtcc tcgtcctttt actcttaacc gcagtcttca
    18961 gagcagtcgt tctgctttgg ccgggggctt tcaccaccga tcccgattct tatcagcggg
    19021 tggcacagaa cctcgcgagc cgcgggattt ttgcccttaa tggagagccc accgcgtatc
    19081 ggccgcctct gtatcctgtc gtcctttcgg gaatcatgtg tgccgtgggg ccgtactggc
    19141 gaataggtct cggtattatc cactggctat tcgggattgg taccgtcctt tgtgtgatct
    19201 atcttgttcg ccgccgatct tgccgggttt gcgctctggc agtgttattt gtgatagctg
    19261 atcccattct gttgcaccat agccgtctga ttatgacgga aacacagagt gtcttcctgg
    19321 ttgcggtgac gttagcgtta tttcatgccg gcacgtctcg cgcagagaga atctggtggt
    19381 ttttcggcgg tgtcttcctg ggactttgct ctctgacgcg tcccaccttt cttccctggc
    19441 ccatattagc ggtattactt tggcttctgg gctcagtgca actgagtcgc gacacttctg
    19501 acatgacaag acccagtgtg tccgatgtcg atagcttacc accgaaagct catggccgcc
    19561 ggctggccgg ggtaggcaga atcggcttgg cggctattgg ctttgccgtg gctattgttc
    19621 cttggggtgt gcggaactgg ctgatccttg gacgaccgat tatcggaaca acacatggcg
    19681 gttacacgtt ttatttggcg aacaatcctt ggtattacaa gtaccttcgt caacccggag
    19741 tgaaaccggt gtgggacgcc actgagttta acgcccaatg ggtaaaaacc cttgaagcga
    19801 agcagttaca cgacgagttg gcggccgatc gcctggccta cgagcttgcc tttgagacga
    19861 tccgccatca actgacgatg tttctcaggg cctgctatga tcgcttctgt caactgtggc
    19921 ggctcattcc caatcgcttg gaccaggatg aaccgctcgt cttgtgggga ctgcggcacg
    19981 gcgtggggct attttatctt gtggaatacg ttcttgtggg attgggaatc ctcgggtggc
    20041 taaagaggcg tgattttcag accccagatc gtgtcctggc cgttctggtg tgcgctcttg
    20101 tgctgaccgt tgtgggcatt catcttttct actggaccaa tatgcggatg cgggcgccgt
    20161 taatccccat gcttgctctt ttggcagggg aagggtatca ctacctcacg tctcgccgat
    20221 tggaaaccga acaaacctaa cggtctcgct tattagggcg attacgggga agtcgtcgat
    20281 atctgggaag gaagggactt ctgcgatctc ctttggaggg cagcctcggc ttgttcgatg
    20341 tacggctttg cccagccctt gtcaccgtag aggacgatta tcgcgcggcg gatggcgtcg
    20401 gccttctctg gatcggtagc ttcgagctga ttggcctctt caagccgccc ggcgatcaga
    20461 tcgcgctggt tggtgatcgc cggcgccagt tgttttttca gttgttccaa acgtcggcgg
    20521 gccagttcaa ggcattcggc cgtatggcct acagggcgac gcgatttgcc ttgaggtgtt
    20581 tcgcgcgact tgtcggcggg gagcttttct gtgggtgcat aatcgtaaag ggcaaggagg
    20641 gcctcgagct tttcaattcc aagttcgggg tcgagttgcg cgtaacggag ggcttgaagg
    20701 tagtctcgtt cgataggaat cgatgaatga gccgaggcct tttggcgact ctgcaattca
    20761 aaccgccgct ccagacggag aaggtcgatc tcttgttgat actcacggat agatggcgct
    20821 cgggcgtcct ccggataccg ggttaggaag gcctcgatat cgggcgcagc gctgaggagg
    20881 gcctcactat cggtgccatc acccacctta gccatcaccc gattgtaaag ctggtcagcg
    20941 gtcggtggtt gcagaaaata ccaaatggtt attgccagca cgacaagagc agccagcagg
    21001 attcccgttt ggagggagaa gattggttga cgctcactgg gggaaggctc aattttatcg
    21061 agctcttcgg ggggaacttc gacaaatttt cgggtggttt gggttgcaga actggttacc
    21121 ggcggggacg gctgttctgt tcgtcccttt tgttccttgg gttcagccgt ggcagtggaa
    21181 cctaaaccat cttctggatg gtctggtact ggcgattctt tttccaagta ccgaaagaca
    21241 tcggtctcac gcgtcggctt ccacatcgag ggcgttccca cgggaaaggc agctgcctga
    21301 ttttccgggg attcactcgg tgaacctgcc gccgtgatcg tttctcccag cgagggcctc
    21361 ggtggctcgg gcgacgaagg aagggcagcc gtcggtgcct caccatcgac gggcacactg
    21421 cggcgatctc cttgctcgac accgaaatcg atgaggtggc cgggagttgc gtgcaagaca
    21481 tcttcggagc cagccacttt gtgcttggtc agggcgtgca gcatggcctg caatcgccga
    21541 cccgccaaaa gggcgttggc aatcctcttg tcggggtctt tggcaagcag ttcgcgaatg
    21601 atgcggtcca attcttcagg acaatcgggc acaaagctcc gcactggggc cggctgcgat
    21661 gtccgatgga gatggaggat ctcggcaaac gaggtaacct tgaacggcgg gcgcccaaca
    21721 agcaaggcgt aaagaacggc gcccaaactg tagaggtctg ccctcgggcc gatcggcaag
    21781 gattctgctt gttctggggc catatactcg attgtgccaa ggatgcttcc tacgccggtg
    21841 tggctgggtg caccgaaaag ccgagcgatc ccgaaatcgg agagtttgat ggtcccgttt
    21901 tccgcccgca acagattggc tggtttcaga tcccggtgga tgatcccgcg atcgtgggcg
    21961 tgtctcaaag cacggcaaat ttggacaccg atttcggcga cttcctgcca atgaaacttc
    22021 cgcccggcac gcagttcctg gtcaaggctt ggcccgtgca cgagctccat gacataaaag
    22081 agaattcctt gttcttcacc aaagccaatg aggcggacga tgttgggatg attcaacttc
    22141 cgcaacgtct cgatttccaa ttcgaaacgg gcacgaaaat ccggttcttc gatgagggat
    22201 tcggataaag tcttgaccgc cgcaggttcg ttggtgacga cgtgtacggc ctcatagaca
    22261 gttcccattc ccccgcggcc gaggcgccgg acaatccgat agggcccgag ttgttgaggc
    22321 tcgttcatgg caggttcact tcaccctgca agcatatttc caaggcaata agatcgagag
    22381 cccctccgta tttagtgtaa cggcttctcc agaaaaggcc agaaaaaaga aagggacgca
    22441 tgcatctgcg tccctaagct ctcgaaaaac ccgattttga ccttacaagc ctatcccaaa
    22501 gttcgctctc cctctggcag gaagttgggg tatgggcaag cgaccgtcgg agtcgattga
    22561 gaaagcggca gcgattggtg ccgccctcac ccccggcccc tctcccgccg aacgggagag
    22621 gggaggttct gggataggct cctaactcac cgcacaaggc ggcaaaggct cacgcaatca
    22681 ctcgaggacc ttgatcctca cattgcggaa gtagatcggt gaatcgccgt gtttgccttg
    22741 aagaccgatg tggcccttct ggggcagctc ggccttgggg cggcttaacc aggctggaat
    22801 ctcgcttcca tcgggatttg tcttcgcgga ggtccacttc cgcatgtcca tttccgtcac
    22861 caactcgccg ttgaggacga cctgaatcat aggcccacgg caagtgattg tcatccggtt
    22921 ccattcgccc ggctttttca ccacggattt cttgggggcg agatggccaa agatagctcc
    22981 gcaatgccag gtcgcggggg ccttggccca cttttcggca aaatcgtcgg cgatctgaat
    23041 ctcgaccgaa ttgggaatcc aattcttgat atcgctgcaa tacacaatta cgccgctgtt
    23101 cgtcccctcg ctgtttttga actcaagatc gaggatgaaa ttcgcgtatt gatccttggt
    23161 ccaaatgcac tggtcctcag tggccgtcag cacaccatct tgaaaagccc aaacgccggc
    23221 aggaaagatg gcattagaga gatcgcgggc aaaaagctcc ggccattggg atgagtctgg
    23281 gtgtttcgac tcatttgccg actcctgggc cagcacctct gcgccgattg acacgatcag
    23341 taccaaagat aaccgccaac accaagccag catgttgcga aacatagaaa ggccctcctg
    23401 tggaattgga atcgttacaa aaaacgagcg ggaacctagt cacgcggtac ccgtttgact
    23461 aaactaagcg cattatgact ttggtaatag ttcccgcaaa tcacccacgt tggtttaaga
    23521 taccgcatga cctgggtgcg cacaagtaac acaatgtgga gctttcataa gcgcgagaat
    23581 gcgtccattt ataggaatgc caaagagatt tgatccctcg tacactgaga aaagtcttga
    23641 cgatgccttt gttattttat catgagtggt aagaataaat ggaaatgggc gaactgaagc
    23701 gcggtcgtca agacgggagg tagctactcg tggaacacct actcgattat tcgtggaaac
    23761 agttcagaga ttggtttgtg agcaagggac aaccaggtta tcgggttgct caagttctcc
    23821 actgggttta cgaatcgccc aagatttcct ttgacgagat gtcggacgtt ccgaaggttg
    23881 tccggcgaga gctgaaaaag tcgttcaaga ttttcagtat gcaggtagcg agccatcgca
    23941 aaggctccga tggcaccgag aagttgctct tgcgaaccca ggacggtgaa ttcgttgaat
    24001 gcgttttgct ccgcgacgac aaaggaaacc gcacggtgtg catcagcacg cagattggtt
    24061 gcggtgtcgg gtgcgcgttc tgcgccagtg gcctggatgg tgtcgtccgg aacttgacgt
    24121 cgggggaaat tctcgagcag attttgcaac tcaagtgttt gctccagccg gaagagcgac
    24181 tcagccatat cgtggtgatg gggatgggcg aacccctggc gaacctggga cgtctcttgg
    24241 gcgccttgca tcgagcaacg cacaaagatg gattggggat cagcgcacgc cacgtcacca
    24301 tttcgacggt agggcttccc aacggtatcc gacgacttgc cgagttaaac ccacagtacc
    24361 atctggccgt ctcgttgcac gcgcccaatg atgaactccg caatcggctg gttcccgcca
    24421 atcgtcggat tgggattcag gccatccttc aggcggccga tgactacttt gccaaaactg
    24481 ggcgacgtgt gacatacgag tatgtcttgc tgcgcaacgt gaacgatcgt ccggagcatg
    24541 cccaggagtt ggccgagctt ttgcggggcc gaaatgccct ggttaacctg atcccctata
    24601 atccggtcca gggtctcccg tacgaggttc ccgatcgtcg cgacgtcggc cgttttgccg
    24661 atatcttgag tcggatgggc attcaggtca cgattcgtta ccggaagggt tccgagattg
    24721 aggcggcctg cggccaactg cggcgcgtcc atcccgagat cgtcagcaag tccaagaaaa
    24781 agaagaaaaa gaagagcgcc gcggccggtc aggaaccaac cggttcatca gaatcgagtc
    24841 cgacctccga taatacttcc acatcggact ccgtgatcat ctcgaatgac gcgacggtcg
    24901 caatttccga tcacgagcta cagatgtatg aggcgaccga agacctgcct gccgaagccg
    24961 agcaggccgc tccggcgaag ccgtctgacc aggtctcaga atagatagcc ttggttcgga
    25021 gtaggataac tcattcctaa gtgttcgtaa gccttcggcg taagtttgcg cccacgaggt
    25081 gttcgcacaa ccaattccga tcggagtaag aacggttcca cctcgtcgat aagtgtgtcc
    25141 ggagagagat tcatcgtgtg ggcaacggct tccactccga ccgggccacc gccaaagacg
    25201 cgcgcgatgg tttccagata cttacggtcc tggacgtcta gccctagctt atcgacacct
    25261 tgcatggcca gggcgtcttc ggcaacctgc agcgtaattc gcccatttgc ccgacttgtg
    25321 gcatagtccc gtacccaccg caggcggttg ttggcaattc gcggggtacc gcggctgcga
    25381 cgagcaattt cctcggcggc gttgggatcg atcttaatct gcagcttttg ggcgttccgc
    25441 cgcacgattt cagccagttc atctacggta tagaaatcga gatgctcccg catgtggaaa
    25501 cggtcccgca gtggggccga aagaaggcct gaccgcgtag ttgccccaat taacgtaaat
    25561 ggcttcagtc ggatgttgag ggttcgcgca ttgacgcctt cccccaaggt gatgtcgatc
    25621 cgaaagtcct ccatcgccgg atagagaaat tcctccaccg atttctgcat ccggtgaatc
    25681 tcgtcgatga aaaggacgga tcgctcttcg gcattcgtca gataggggat caagtccttc
    25741 ggggccttga gcgtggctcc gctggcgatc tgaatgctga cccccagatg ccgcggaata
    25801 cacaaagcaa aggtggtttt gcccaacccg ggcggtccat caaagaggat gtgtcccagc
    25861 ggttcgtccc gtttgagggc tgcgtcaatc gcaatctgca atcgttcgta cacatcgcgc
    25921 tgaccgacca tttcctccat ccgttgcgga cggaggagcc gttcatcctc atccgtacta
    25981 acggacggct tcaaaatggg ttcgcgagtc atttccgcac gtcaatccgt ctttctgatc
    26041 caggctccgc catgctccac cggcttctgc ggggaccaag cggcaatggc ccctggaaat
    26101 gcaatataac agaccagcag aagatgggcc aagagcatcg gcctacggat gcctttttgg
    26161 ccgtgacccg agagggacgc acgccgcgat gtccaatgtg gtacgagccg ccgggccaaa
    26221 ctggcgagac atgtcacctc caatttgcgg cggctcgtga ttcgaggtgg gcgaccgccg
    26281 tgtacgtatt cacgacggac cccacggctg aattcgccgc ttaatcggct ggccttcggt
    26341 atcagtcagt ccgtcagctc gaacgtgaac tccttctggg attcgctgac ctcggccgtt
    26401 aatccagatg tctccggagc agcatacttg gcgggcagct cctcgcggta cgttgcggga
    26461 acaggcgggg catccggatc ggccggattc gaaccaccga cttggattga cttgcggatg
    26521 gtcacacgat atttgccggg aacggcgcca tcgttttgat caaatgtggt caaccgggcg
    26581 atcccgttgg cgtcagtaac gccagtggct gcgttgccac tgccctctgg cagaaactga
    26641 acgttcgctt ctgctacggg agcaccacga tagaggaccg taactttgac ggggacggtc
    26701 ttggggcgac cggggaccga tcgcttgcca ccacaaccgg gcaaagcgaa aaacaacagt
    26761 gcaagtccca ccagagccaa gcacgtcacc gaaaagacat tgcgagtttt tgtcatggtc
    26821 tctcaaaact cccttctcac ctaccgtttg ttgaaatatt gtcagggagc tggtccccct
    26881 gaacggttaa cgacggaaac cccctctcaa tcaaaccgga gagggggttt cactgataac
    26941 ctgaaatccc gatcggctga aacctcaacg aaccaatgtc gttacgggta caccccgatt
    27001 ttacaggctt gccgtttcgc cgccgctctt ggtgccaagg gcaccccaaa caccgtaggg
    27061 gctcatacca ccggaagtga ccgaaggaaa gtctgtgccc aggtttccag tatccactgt
    27121 ttcagagaca aagcggacgg aaccatcggc aaaggcgaca ttgacaccgc ctgggtgacg
    27181 gctgcttggc gtgtaaatgc cccgctccca gtggtcggcg gcattcgcgc aggaagcgga
    27241 attaggcggg agaatggtgg taaacccggc aaaccaggcc gctccatccg cccaacggaa
    27301 accggggggc gtgtcagata taaagctgcc ggtgatgtac ttcttttgag aagcatcgta
    27361 agctgcccgg caagcaagcg ggtttgttcc gagcgagagt ccgacgacat ttcccagcga
    27421 gttttgagca cgggggcgaa gccgttcaga gatcagcgcc gtgttgctcg tgccgtcgct
    27481 gatctcagcc atgctcgacc attgaaagac atggaacaca ccgcggatgc tcgtgctact
    27541 cggcgggtta ttgaagctgt cgatgggtga atccccgccg ttcatgcagt aagtgacagt
    27601 gccccgggca gtgccggtcg ggggtgtcga ggcgggatca ctggggcaat tcagggccgg
    27661 gatattcacg ttgtaccagg aggtgttatt ccatggcgcc tggtggggca aggcattgat
    27721 ctgatcatag agcgactgct gttccatgta ggggcagacg gccacccaac cgctcagccg
    27781 accccgatgg gtatcattgc tgcccgtatt ctgatgtccc gatccagcct ggcgggcggg
    27841 aaaacgggtg tacacatcat ggtaattctg catcgccaaa gcaagttgct tcaagttatt
    27901 ggtgcactgg ctccttcgtg cggcttcccg ggcagcctga accgccggca acagcaaggc
    27961 gatcaagatc ccgatgatgg caataacgac caaaagctca actagcgtga agccccaacg
    28021 aggcttacgc ccacaacgac cgacacatac cgtcatctca caaacctcct ccaaacagga
    28081 acaacaagga agcatctcga aacacagtgc gagaacttcc accaagccaa tgctaaaact
    28141 gaaccgatac ccctgaaccg tctcgggatc acaaaaacaa gatcacgacc aagacgagcc
    28201 ccttcacgtg aaaggcggga gaaactgagg gtgcagttat cgttcccttc catattgcat
    28261 cttaggtccc cggggcacta ttgtcaggcg acggcgcgcc ggtgatttgt atgcgctggc
    28321 tgtaccttga cgctgggggc aactcttgtg attgcccgtt tgggtccgaa acggatttca
    28381 atcacgcggc ctccacggcc cctggcccgt gcaggcgatt gccggtccca cctcaaaaac
    28441 ggggggacac gcatcttggt ctccgatgtt gcaggtgccg ccagcggaaa attctcactc
    28501 ggcgctcccg tcaaaacctg ctgctgccga ctcctgggca tgataacgcc gggtcggggg
    28561 gattgtggcg cggcggctat cgggataacg tgaaaaggat gtgccaaggg caggaaagat
    28621 ccatagtgcg cgcttacata cagcgtccca acgggtttat gtgactgtac tcacagtcga
    28681 aggtgttttc gggagatgcc atcgatgttt gaagaattcc tccaggcagc gcgttcgatt
    28741 tttgagcagg ctcagcacga aggccggttg attcgcaatc cagacacggc ccgactccgg
    28801 gcaatggccc ttgaggagcc agaggcccgt gagacgtgct acggcaacgt ggccgtggaa
    28861 tcggagccaa cttcccgggc tgccatgttc acaaagaaca atatcgatac gagatttggc
    28921 aaggaagagt atgacctgtt ggaaaaggcc aagcgggagc tttcaaaaaa agaattggtg
    28981 agcatcgacg tgcaggtcgg agatggaacg gaaggcgtga ccgcccgcct catcgtcccg
    29041 cgagattttg cccacgtcgc ctttgccggg ctgaagcttt tcaagccaac gagcacggac
    29101 gagccgacct atcaggtcat catgttcacc gatgaagcat ttgagagtaa caaaaagaag
    29161 aagcttcctg ataaaaagaa gaagcttcct gataaggaca ttaccattcg tttggcgcat
    29221 gccccggacg ggcggatggt gaagttcgtt cgaaatagca attacttcgg tgagtggaaa
    29281 aagggtgtgt ttgcggggga ggactggcgc gtaaaacaaa aaggggacgc aatcttcctc
    29341 catgtcggtt gccgtcgcga tacactggaa acgccccatg gtggatatcg gacggtttgt
    29401 tcgctatttg ccgctctgag tgctaatggc aagacgagca ccacatgtaa ggttttggcc
    29461 cgcaagggac gtgagcgctc ttggctaatt caggatgatg gtggtgccct tcatcgcgac
    29521 gggtcattcc gtggcttcga gccgggggga atctttatca agacggatgg cctcaatcct
    29581 ggcgaccagc tcgaagccta ctatgcctgt ttgaaacgag aaacgttcct ggaaaatgtc
    29641 tggatcgagc ccgacgggac gattgacttt ttcaatatgg aacgtacctc caacggtcgg
    29701 gcggtggtcg aacgccgcga ttttatgcac gctgctcaat ccattaactc ggagcgggtc
    29761 gataatttgt tcctcatcac gcggggtagc accatcccgg cgattgcaaa attgacgcac
    29821 gaggaggcgg cagccttcat ggtcctgggc cagtcgatgg agtcttcagc cggtgatcca
    29881 acccaggcgg gcaagatcaa gaattgcttc ttctacgatc cctttttggc gggtgatcgg
    29941 gccgagcatg ccaacctgtt ttacgacatc ctcaaggcga acccgcacat tcaatgctac
    30001 ttgctgaata cgggttatgt tggcgaaggc gagtccatgc gggacattcg cctggccgac
    30061 acgatgggca tcctcgattc cgtgttgcgg ggtggccttg atgaatgggt gtactcgccg
    30121 cgaaccaagc tatatgtccc ttcggcggtg aggctggttg actccatcct cttccatccc
    30181 gagaaattgt tcccccacga tgtctttgag gaaaagcaaa aggcgctgga aaagcaccgg
    30241 gcagagtttt tagaccgttt ccccggactc cacccgaata tccgtaaggt ttttcagcgg
    30301 tagcgggttg ggccagcggc gcttgctatt tacgtgaatc ctccttagac tgcggagaac
    30361 ccagggtggg ttgccgggag cgtttgcgct ttcgggatga atttggctta ctccgaggtc
    30421 ggggaggttg gttgtgaaaa ccctgctgct gatggtcgct ttggtgtggg ggacgttgtt
    30481 caccgttggt tgggccgtcg cccaaatgcc caagcagcca cccaacgtca tcattctcat
    30541 caccgacgat cagggattcg gtgatctttc atgtcacggc aatcccgtct tgaagacgcc
    30601 gcacctggat cggttgcatg acgagagcat ccgcttcacg gatttccacg tggcgcccat
    30661 gtgcacgccc acgcgggggc aacttatgac cggtagggat gctcttcgca acggggccat
    30721 gaatgtgagc agcggccgca cgcctctccg ccgcgagttt cccaccatgg cagaggtctt
    30781 tgctgcctcg ggttatagca cgggcctctt tggaaaatgg cacttggggg acaattaccc
    30841 gtatcggccc caggaccgcg ggtttcaaga gagcatctgg tatccgtcct ctcacatcgg
    30901 ctcagttccc gatgcgtggg agaacgacta ttttgacgac atttatttcc acaatggccg
    30961 tccgcgtcag ttccggggct acacgaccga tgtgtttttt accgaggcca ttcgctggat
    31021 ggaagagcag gccaagcagg ggcgaccctt cttctgttac cttgccactg cggcacccca
    31081 cgcccctcat ttcgttccgg aacagtaccg taatgcgatt cgccaatcgc ttgaggccgc
    31141 acgccctcag ctttcgaagt tcgccgccgg gttgcaaccg gaagcacgta gctcgttcga
    31201 ggaggagttg gtccgctttc tggccatgat tgccaatatt gatgaaaatg ttggccgtct
    31261 ggaaggcttt ctgaaaagct ctggcctacg ggaaaacacc ctgctcatct ttttgaccga
    31321 caacggcagc acatttggac cgcgttattt taatgcgggc atgaaagggg gcaagaccac
    31381 attgtgggag ggtgggcacc gtgtgccttg cttcatccgt tggccgggcg gtgggctacg
    31441 tccagcaggt gatgtcccag gattgaccca ggttcaagat atctttccta cgctcgtcga
    31501 gctgttacaa ctgcgtcttc cggcaggcgt tcaattcgat ggaatcagtt tggcgccggt
    31561 gttgcgcggg cagagcgaag tgccagagga ccgcatgctg gtcatcaatt acagccgaat
    31621 gcccttcaag agcacccgca cgacggccga caacaacgct gtcccacgcc gggaaggcgc
    31681 tgctgtgctt tggaagcggt ggcggttact ggaagataag gccctctaca acctagcgca
    31741 cgatcccctt caagaacgga atgtgattga tcaatatcca gaggtcgccg cgaagatgcg
    31801 atcccatttg aatgcctggt gggacggcgt gaaggcacgg gtaaatgaat ttcaggcggt
    31861 gattgtgggt gatgacgcgg agaatcccac gatgttgacc gcctgcgaat gggccgacgt
    31921 ctttcttgac cagcaggcac aggtacgacg gggggaacga aaaagcggcc tttggcatat
    31981 cgaggtggcc aaacccggcg agtatgcctt catgttaagc cggtggccct acgcttcggg
    32041 gctgcggcta cgcgatggga tcagcgagac caaggtgacc gatggtatct tgccagcggg
    32101 cgaggcttgg ccggtgaccg ccgcaaggat tcgcgtgggt cacatggagc gagtcattcg
    32161 cccggtgggt gatgccattg ccgcgaggtt tgtcattccc ttggatgtcg gtcgaacaac
    32221 cttggaaacg atgtttttgg atgaaaacgg tcaggagatt gccggggcgt attacgtcac
    32281 tgtggaacga cttcggcaag tggagcagcc agcggctgtt atcttagaca cggacatgtc
    32341 cggggattgc gatgacgccg gggccttggc gcttctccat gccctggccg atcgcggaga
    32401 atgcaagatt ttggcaacga tcgtcaatcg gaaggacaag accaatgcct cggcagccgc
    32461 agtagatgcg atcaacacct actacggtcg cggaaatcta ccgattggca ccgacaaacg
    32521 gggtcccacg gacctgcaaa ggacgagtgc ttacgcgccc gctttgcgag atgaattccc
    32581 cagcgacatt ggccctgatg accaggcgcc tgatgccgtg gatatttaca gacagattct
    32641 tgtttctcaa ccagacggaa gtgttacgat ttgcagcgtg ggggcattgt ccaacctagc
    32701 ggagctctgg cgcagcgagc cggagttggt ccgcaagaaa gttcggcggt tggtgatcat
    32761 gggtggtgag tttcccacct cgaagacgcc cgagacgaac atccgtactc accgcgaggc
    32821 ggccaaaatt gttgccgagg agtggccggg tgagatcatc tgggtgggat ttgaaattgg
    32881 tcatatcgtc ttcaccggag aaggactcaa attaacgccg cgccacaatc ccgttcgccg
    32941 ggcctacgaa ctgcggccgt ttggaggacg gcctgccatc gaaggtggcc agcccagtta
    33001 tgatcaaaca gcggccttga ttgccgtccg tggcctggag ccggatttgt gggaggtggt
    33061 cacaggtggc cgagtggacg tggatgcgga gggccgaaca acgtggcgag ttgatccgac
    33121 gggcaagcac agctatgtcc gcttggcatg cccgcctgca aaactggcta ccatcattga
    33181 aaatctcatg atggctcccc ccgttgccca catggctgaa gagaacgacc cacgtcaata
    33241 ggcagggccg acgtatgcct cgcctccacc gctgatatga gccactgcct tggtagtgtg
    33301 gtggcgaaat gttgccactg gcggttgccg ggttttgaat caccgacgac ataatgagga
    33361 gcgccctgag caaaatcacc aaacagtcaa cccggcgtga caagccgcta aacttgcgga
    33421 gaccatgcgt cggtgatcaa gaatctatat aatgcaccag cggaaattgg gaaaactcta
    33481 acgtggtctt tgaggtgacc tagagaacat ggggggtaac atgattcgca gtatgcacag
    33541 agcccgccgc cgtacgttga tgaaagacac gcgaaaagta gtgaactcat tggcaaccgt
    33601 cggcctggtc atcatcgcag cgttgattcc actggccgtt gtctgggcgg agtcagcgtc
    33661 tgcggcgtcc tcggaagggg ggaaggtttt caaggcaggt gccgcggcca gtaatatcac
    33721 gccctggtta gatgagggca tcgtcggggg atggtcatcg ccgccaggca agtatatcca
    33781 cgatgagctg tacgcccggt gcctggttct cgatgatggc agcacgcaat tagcgattgt
    33841 cattgttgat aatctggggc ttgcccggga agtatgcgat cgggcaaagc aatcgattcg
    33901 cgagcacacc ggtcttccgg aagaccatgt gttgattgcg gccacgcata cccattcttc
    33961 aatcagtgcg cgtcctccga acgtcctcaa accggaggag gacctgaacc agtatgggca
    34021 gtttgtggcg cggcggattt ctgacggagt ccggcgggcg atggcacatc ttgaaccagc
    34081 ccggatcgcc tggggcacgg tagatgtccc ggaacatgtc aacaatcgcc gttggttcat
    34141 gaagccggaa attaaattgg tcaatccctt tggtgggcta gataaggtgc ggatgaatcc
    34201 gccggcaagt agtcccgatc tcctcgagcc cgccgggcct gtggaccccc aagtctgctt
    34261 tatctcgatt cagtcgcgtg acgggcggcc aatcgcactc ttggccaatt actcgctgca
    34321 ttatgtgggc ggcgtcggac cggggcacat ttccgccgat tactttggtg tctttgccga
    34381 ccgaattcag gagttgttga aagccgatcg actcgaaccg ccctttgtgg gcatcctttc
    34441 aaatggagca agcggtgatg tgaacaacat ttccttccag aaaccccggc ctgcaaaaaa
    34501 accatacgag cagatgcgct atgtggccta cgacttagct cagaaggtgt tcgaggcaca
    34561 ccaggatctt cagtggcagg attgggttcc cttggggatg ctgcagcaag agcttgtcct
    34621 caaagttcgc aaaccaacgc cggagcaact gaagcgggca aaagagatcc tggccggggc
    34681 gccgcctttg ccggagcggc ctcacgaaga gacctatgcc cgccggctgg tccagttggc
    34741 agaatcaccc gacacggtct ctgttccgct gcaagccctg cgaattgggg aagttggtat
    34801 cacggccata ccttttgagg tatttgccga aatcggcctg gagatcaaag agaaaagccc
    34861 cttccggccc acatttacaa tctcccatgc caacggttcg tacgcatatc ttcctacgcc
    34921 ccggcagcac gaactgggcg gttacgagac ctggttggga acatgcaaag tggaagagca
    34981 agcatccgtc aaaatcaccg aaacggtcct cggcatgttg cgggcacttc gcggagaatc
    35041 cacgaacaat ccttgatcac ggcttgtctg tggtgaccag gctgagttta ctggccgggc
    35101 catttcggta acggatgcct ttgcgtaaag gcgcggacca cttcctcttg catggtgccg
    35161 gggcgagggg tcgtgaagac ccaaacgatc cccgcctgtt caaaggccgt cttcatggcc
    35221 tcttccagat aggccttcgt tgggcggccc tcagtccacg aggaatgcgg cgtgaaatag
    35281 atgcacggaa tcgcaatttt gcccgtttgc aggtaatcgt ggatttcaag gggtaacgtg
    35341 gttgcgtcta gcccacactc agggtaacgg gcggaactat gttgataacc ccataacagg
    35401 gcatccacat aatccaaata gccagctttg acaaagtttg ccaggctttc gtgatacaca
    35461 caaacgccga acaggaggcg ggggttgatt tgtttcgacg ttttgtaaat ttgctgggtg
    35521 tattcacgcg tgaaaaaccc taaattgccg tcgaaatcgt cgatcatcca aagggtcagg
    35581 ttggggtgat caagcgagac ttgggcgacc gcctcggccc agcgcacgta atccatgtca
    35641 aacgggggat aacgcctctc gcggataggt ttctctcgat cgataggggc ctctgacggt
    35701 ggcacgagat acacccagac ctcgatcccg gccgatcgtg ccttctggca aaattccccc
    35761 agggcggcca actcttgtga ggaccgcggt gcgatcaaat acgtgtaaac gttaactcct
    35821 aagtgactca aatgaccgag catggcctcc acgtcgggcg taccatccgg tttgtagcgc
    35881 ataccggagt aagccgagac tatcccgcga taaaaggcgc ttttttttgc ccgctcgttt
    35941 ttgatgcgaa gattgtaggc gacggtgggc tccaggttct tcacctgaat cttgccggaa
    36001 gctccctgag aacctatcgc ggcttcgaga tcgtaagtcc cttcgggcag cgccggcagt
    36061 tctaccggat gattactgac acgggaaccg gccttgggcg agatgggtaa ttgccattcc
    36121 ttgagcagtc tgccgccatt gtccttgata gagaatcggg cagtaagtgt atcttgcgaa
    36181 aaaggccgat tgctgaccag aaccagcgag aagctgcgac tctttcctgc ttcaatgaga
    36241 gagggaacgt caagccgaat gatcgttcgg gccggtaaac cattagcgat gggttggagt
    36301 acgggaccct gttgaccgct ggagatgagc ttgaacctct cgttgatttc ttgggcggag
    36361 agtgcccgac aatagatgcg gacctcatcg atggacccga ccaggggcca agagctccgc
    36421 cgaccggcgc cgaagccgcc gatcttcaag ggggccgtat tatgccctgc cggttcgaag
    36481 ggagtaaccc gctgggtgat cagttcacca tcgacaaaga gcgacaactg cttgccagac
    36541 cgcaccccga ccacctggtg ccagcgaccg tcggcgatgt ctggcccttt caatctcacc
    36601 tggaattgat cgttgtactc cgcccattgc acacatcctt caaagaaacc catccaccaa
    36661 gcggaaccca ttcctgtacc cgtccacttg gacaggtaga tttggggtgt cttctgatcg
    36721 tgtttcaccc atgcttccac tgcgtaatcg tcagagagaa caaagaattc gtcgaaggct
    36781 tcggcaagca tcacggaatc gctccctttc aagacgagag cctgaccgct gatgccactc
    36841 ccgcgccgtg cggattggag caaaaggtgg aagtcgttgc ccgacatatc gtgggcaacc
    36901 cgctggtcgg attcatcgaa gctccaatag gccaggaggt ggctttcctc ggcctggcac
    36961 agtgaagaaa aggcaaagca gaccgtggcc gccacaacga cccaaaccca tgcccgtgat
    37021 ccaagccggt gactcacccc ttgcgatgtc cacaggaggc gaagcatagg aaacctctca
    37081 ctttgctctt tcggaacgtc aaatctctgc taccaacgac gaaccggaat atgcttggcc
    37141 aatcatttac ggtgacaaag gatcgcccat cgtgatcacg gtgaccttta cccgtgggtg
    37201 cacgcccggc gggacttccg gctggcaggt gaaatgaatc gtgttattcc ctgcgttgag
    37261 cacgggaacg gtgccttcca catcgactgt ttgcgtgagc tttccgcgtt catcataaac
    37321 gcgggctgga ttctgaccat cgaattccag atatccaccg ctggtcaggc caacaggaaa
    37381 tatcaggcgt tgcccattta gttccagcga cggttgccca atcttgatgg aaaccacggg
    37441 caaggttcgg ataggactga tcagacacgt cgtttttccg tttgcgggca cttcattgat
    37501 gaagagattc atggcgctaa cgtggtcacg aatcagcggt gatcggtaca caacggcata
    37561 atagctgccg tatggccatt cgtagcggtg aaaatcatcg gcgtcgcgct cccgcaaaag
    37621 aaactccaca tagcgccagc cggtgaagtc cagccggaca tagtgctcat cacatgttgg
    37681 ccagtaatgg aggggattcg tcaattgcaa gttgagaagc gccccgctcc cgtccccatg
    37741 aatccaaacg cccatggccg cgcgcttttg catgttcacg tggggatcga atgttttcgt
    37801 gaaatgagcc cacgccccgc gggaggtgcc ccgggtattc tcggcgacga gcttgccact
    37861 gctcgaacca accttgatct catcggtaac atacgatagt tctgctttaa caccagcagc
    37921 ctgggcaacc tgagagaact cgccctcctt gaaagagaca atctcctcag cattagcgct
    37981 gtcatagggt tcgaccccat agagcgcctg aatgcgaacg cggagcggct gggcggaata
    38041 tcggttattt actgtccatg tcgtgctttc cggcacggcg cccgtgattt tatggctcag
    38101 ataatcggcc gggcgaaatg cccatgaacc atcatcccgg atttccaaat ggaaatcttg
    38161 cccttcctgc ttgaggcgtt cacgaatttg cggtggcact tggttggaga gccgaaggtg
    38221 ctcgtatcgg cccaacattt cgaggtactc gggctggcgg gcgcaagggg ggttatcacc
    38281 cggcgcgacg ccctgaaacg acatcggcat cccgtaggcc agtgacttcg cgcagaggta
    38341 ttcgaactcg tcggaaagct cggcatcgtg atcggcggtt ggccccaaaa tggcccacca
    38401 gccaagttgg accggtaata gcgagctgcg acgatgttgc tcattcgacc ggcaatgcca
    38461 atccacaaag ggtttcaagc cccagttggg atgatcccag gccccaaggc gagaatgaaa
    38521 tgcccaggaa tggtagtccc aacaactcgc ttccaccagc gcaggacgct tcaggcgtcg
    38581 gaaaatctcc tctctcatgc ggctaatccc ataccaaccg ccgggcatgc cctcggcgcc
    38641 gtccatataa atcatgtcaa agccacatgt gttatagacg tcggcaatgg cctgggcgat
    38701 ttcctccacc agcgtcgagt tctcgtccgg caaaaagcac ccgtatcgca caaacagatg
    38761 ctccacaggg gcctcggctt cgtgtttttg cggctgcgtt ccaaaggcac cacggcggca
    38821 gccagtaaac ccgtagggtg gttcctgcga taagccgctg tactggatca actcatcacc
    38881 gatgcgaagc acgttcccat gtccaccata ggcccaaacg gtgtccagat tgccgggctt
    38941 ctcgcgggtc acaatccggt cagccttctc atcgagggga tcaaccaagg tgaatcgtac
    39001 atcgacggcc agccgcgggt cgggcttggg ggtcacccag gggtctgacg gagagatgca
    39061 cccggtcagc gtgtgcatac ccgctttaag ccccgctgcg tggatctttt ccacgacttg
    39121 tttaagtcct tcgattccgt tgggaaagag atttttccga ggctgataat gccccaggct
    39181 ttgttcccaa ctgatcatgt gtatttgggc gatacctgcc tgttttgcca gagcgatcca
    39241 cgcatctgcg ttttcttccg aaactgtggc aaatacatag gagccttgcg tctcgggcgc
    39301 gtcctgggcc cagggaccac caagacggga gcgcacggcg tcttccgtct ccagtacctc
    39361 ttggagcacc gttcgcactt cgccgaccgg gcagcccacc agggccacag ccgcaccgac
    39421 caaaccacgc tccttggggg ccacggcggt gacgaaagcc aattggctgg gcattggctc
    39481 agagtcggct ggcgcggtaa gccctgaaga cgaacgccca ctcagttgcc caagaatcag
    39541 caggttaaga ctccgcacgg ccaccgcaaa tcggtcatct tgagcaagac cagcaatgtt
    39601 gctgatcaat tggccggggc gtaccaggag attgaacagt cgaagttcat ctacttctcc
    39661 ttccacagat gtaacctcaa aggtgaggtg tttagggcga attctggttt ggaggaccac
    39721 acgcgcctgc tcaaattgga ccgtcaatgt gtcattgtct cgaatgcact tttgcggcac
    39781 aaacgttcgc cccgcctggc ggatggtcgt gaaatagccc ggctgggcca gaattaactg
    39841 ctcacgattc cctttcttgt cgaagaaact gctgttttgg ccattctcgg cgatcttgta
    39901 ccgaatgagt tcattctcaa aggtgatctc tcctccgaaa gcgctaaggt ctcctggcat
    39961 caaacagata atcgcgaaga ttcccacaaa gcaggagaaa acccggtttt tagagcctgt
    40021 cccataattt cccctcgccc gttcggcggg agaggggtcg ggggtgaggg tgactcgaat
    40081 cgctgccggt tttctcgatg gacttcgaca accgcttgcc ctcaccccaa ccctctcccg
    40141 gagggagagg gggacttttt ggga
//
LOCUS       DAOSUT010000011        39635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_708_length_39635_cov_9.262380, whole genome shotgun sequence.
ACCESSION   DAOSUT010000011 DAOSUT010000000
VERSION     DAOSUT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 39635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39635
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_708_length_39635_cov_9.262380"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..786)
                     /locus_tag="PLD05_01870"
     CDS             complement(424..786)
                     /locus_tag="PLD05_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76204.1"
                     /translation="MNPQQLSTARTFGGLLVLLVLLLLQFLALVPLLVYPAAAVASAM
                     TLGAPLKGNEPFTLILIPRAFAILCLVYPILAIGASMAAWKAFSRRSMKLCLLWALLP
                     VLILGIIGMFGAVWFLLS"
     gene            complement(879..2765)
                     /locus_tag="PLD05_01875"
     CDS             complement(879..2765)
                     /locus_tag="PLD05_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76205.1"
                     /translation="MKYYVIHVGVIALLLSCVAPHDIATILAEERAGRTFFVDSEGGH
                     DDADGQSPERAWKSLERVNAADLKPGDTVRFKCGGVWRGSLIPVSGDEQAPVTYTSYG
                     EGPKPLLLGSRPRNRPEDWVHVGPKLWATLPVEYRLGRERLDLRNSTWRHHQEAGAQV
                     ELTHEGDADNRVVRVTCIASGSAANHIQLWGPAVAVQKGEHLVFTFRARSSKPFMLPP
                     VNLLKAGFPYSRYAQSTLPKETVGPEWATYQIPLRASESAEDGGLHINLGGIIPAGAI
                     FEFQAQTLHEATPSVPDPLDTDVGNIIFDNGAICGWKKWSLDSLEKPYDFFYDGTSQR
                     VFLFSEVVPTATHRSIELALTRHVVNQSGKHHVIYDGLAVMYGAAHGFGGGGTHHIII
                     RNCDLAYIGGGHQHTRPDGKPVRYGNAIEFWGAAHDNLVEGCRIWEVYDAALTNQGRG
                     TDSHQVNITYRNNFIRNAEYSFEYWNNPEEAVTENIRFINNTCVDAGKGWAHAQRPDP
                     NGSHLMFYTNTAATKGIEIKYNIFAGFTDWGSRYSSGWKVLPDMDYNLWFSTTGVMVH
                     WFGEKIPDFDTYRQKTGLDAHSVFADPRFVCPAEGDYRLAPDSPARTLRPDGGPVGAT
                     MFWE"
     gene            complement(2998..4926)
                     /locus_tag="PLD05_01880"
     CDS             complement(2998..4926)
                     /locus_tag="PLD05_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOP76206.1"
                     /translation="MKRIIVIVGMMFSLLSLMQADGQQRPREMPREDVVDVPAISEGL
                     CVSNLFQTNMVLQRDKPINIWGWANPGEKVTVSFAGQEVTTQAREDRSWQVTLNPIPA
                     NSTPQTMTIRGADRTITLENILVGDVWLLGGQSNMEFPIASVDDGELEIVSANSPQIR
                     LLTVPQGKGFDSVRSFERLYEWSSWSNRHFRKGDWDVCSPETVREFSAIGYVFGRRLH
                     MATGVPIGLIDTSIGGTTVETWTPLEVLEQIEGRETREMLEDWNARIAAYDPQADLQA
                     RIAAYEASIKARKERGEPIPPNSAPPSDLRPGPAADRNRPGYCYASVIRPLEGFSIKG
                     VLFHQGFNNCFNGSAGARMYYQVFGRMITAWRAAFRDPQLAFCIIALPTAGEPQTAEN
                     FVQAMYDVGPYIREAQYRTFRNFYDAGDKAIGYVSTYDLRKSWYHPQIKIPVGERAAK
                     WALVTQYGILSGRDAEQYWLPPSITRVEAVDGTLRLYFSTEVRTKDDADGKLLGFAIA
                     GEDRRFFPADVRYYTEKSDDGQNQPREQRHILVLSSPFVPQPVHYRYAWARNPMGNIV
                     NSRGVPLATQRSDNWILEETPEKIPVPQGMSPETAQRYIGNRLRELLKQADLERRYLE
                     AKRTLQELAPLVERGKTN"
     gene            complement(5044..7758)
                     /locus_tag="PLD05_01885"
     CDS             complement(5044..7758)
                     /locus_tag="PLD05_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76207.1"
                     /translation="MRRSFVKTCFPIVPGFLALWLVVQWTSLSIEARGHEQPNPSDSK
                     FIGLDVQTITNFGGRVAGTVRSQEISGESFVFLGSEVMLVLPAERLLTPEQGSISFTV
                     IPTWPGNDQRAHTFVHMAIGPCHVTIFKTDTNTLRFTYRGREEVHAACDLDITDWQPN
                     EPHEISAGWCKGYGDLLWIVLRVDGREALTHRAVPLVTLPEELFIGRRGPSAQPAEAW
                     IGKLQIRPVAPVLPFAAGPKPPVMAEVDCSVTQPFRRVHDFTTIWNSQENPVPFGVGS
                     PEFRLFQQGGFRMARLVAFSEGWLWGTRVTRDETGRLVTDFSDFDRLLSVITAAGAEP
                     YIRLAYHTPSALVSPSTLAEHRRYALPENLELWDELMERIVRHVLFEQKLPVRYWVTA
                     LNEGDLPVARGQAEPETIYQLYERTSRIVKRLDPQAKVGGPALARSIDLEGRPMPMFV
                     DFLRYCRDRDVPLDFVCFHGYGFAHPRQYEGLVAGVRSAVQTVWPEKAPSVELFLDEW
                     NLWSRTGEQDNEYGAAYLAAALHYQRRSGLTKSSIVSFNHFLRTQAEEATLFEKVGPF
                     ARTADSPVCFQPGTYTCAGRSLPGVRLHPPNGQDRYTFCRVEVQVPSEGNPRLEAAVG
                     VVELPTLSDGCGFQVVALSGDKLTHLMETFQRPGPWKEIRVPLGPFAGQRINLEFRTD
                     PGPKGQSTADWAVWADPRIVVESDHGEKVIWRLMDHLETTVSGARRAGRPFVYNDQTI
                     ALYSGLPLIKGQVVTTPYFVWAMHHRLAHGELRVNLPGRNGILDDDTGGLTATADENR
                     IVLLLWHFDVMQDSPRSWQIRLKKMPPAMSQAKQFRCTEYRIDREHNNPYTRYVRRGE
                     DSQGGAYNLETASLEVTSKSLVRTDAGTASLEVVLPNMAVSLIEICPE"
     gene            8366..9298
                     /locus_tag="PLD05_01890"
     CDS             8366..9298
                     /locus_tag="PLD05_01890"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOP76208.1"
                     /translation="MKIFRRLEEIRGLEGSVVTIGNFDGVHRGHLRLLEVLRKAADDM
                     KLPAVVMTFDPHPAVILRGVEPGPINWVERKLSLLSQAGVDIVLAYPTDRRLLELSPE
                     AFFYEVLRDQLGARVIVEGEDFRFGRDRTGDTEMLRQLCEKEGIGCTVVPPVVWQGAP
                     VSSSRIRECLMCGDVVEAAQLMSRPFRTCGEVVRGAGRGRTLGFPTANLEKVRTLLPQ
                     PGIYAGAVPLGNQIYPAAISLGGNPTFDESQMKLEVYIVDFTGNLYGREVQVDFLQRL
                     RDIKRFASAEELIAQMSDDVEKTREIVRRCDMIQ"
     gene            9415..10440
                     /locus_tag="PLD05_01895"
     CDS             9415..10440
                     /locus_tag="PLD05_01895"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOP76209.1"
                     /translation="MGCEDRPKMAAAPHSIDWSRLINYLESHRSFVLTTHIRPDCDAI
                     GSQLALAEILRNRGCQVACINAFTIPPPLQFLDTEKQLMRVDQPEASRCLEQAEILVI
                     LDTTAWAQLGDIGPVIRQFPRKKIVIDHHVSGDDLGAELFKNTEAEATGRIIAELAAQ
                     WGVALTPRLAMLLFAALATDTGWFRFNSVCAETFQLAADLARAGVRPDALYRQLYENE
                     RIGRFRLMGRAMSRVETDLDGKIIYSYIRSSDFSETGALPSDTEDIINTTLSVAGTQV
                     AIMFIEHTPGQFKVSLRSRCHLDCCRLAGEFGGGGHKSAAGASLTGSLDSVKARVLDA
                     VRRAMQE"
     gene            10619..11248
                     /locus_tag="PLD05_01900"
     CDS             10619..11248
                     /locus_tag="PLD05_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOP76210.1"
                     /translation="MAQPERPTGDAQPACCAAQKASDLQQGGEGGSHVPRRGILGSLV
                     AWVLGLFALGPSFVAGIAAFLSPLGGKAQKGVLLRLTTLDNLPKDGTPRKFPVIAEKR
                     NAWTFSREPVGAVFLRLLPNGDVHALHVVCPHAGCAIEYRPAQSGGQEGQFVCPCHLA
                     HFDLSGKRLDAVSPSPRDMDTLRVEVRDGEVWVEFQNFQLGTADKKPVA"
     gene            11464..>12747
                     /locus_tag="PLD05_01905"
     CDS             11464..>12747
                     /locus_tag="PLD05_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOP76211.1"
                     /translation="MGWALSGDDHVIRRVSRRHQHLESKGTDMTFGDWLDDRIGWRGI
                     WRASCGGGCDPRGRCWWPISLSVIFFLLVQQSLTGFFLWVYYSPSSQSAWESVYFIQH
                     HITLGWLLRGIHFWGAQVLVGFLAISLALRIFSGFYRAPREWVFWTGLVLFAFALGSC
                     LTGDLLRWDQEGYAATQTRVSFLMLLPKVGASLYRLAAGGPEFGHHTLTRFFALHVGV
                     LGFGMWLFALAHAALARRASRAVETSPEAYKNARPDPRFPVVVQGLVCLLALVVVLLF
                     VFHRGLPGIGEVSAWKSPQTSFGAALGAPADLDPAHFYGAARPEWSFRGLYGFSNVFP
                     GELKILPIFVIPGIIAVLVLAMPILGRCSLGHYWNMLLTVVLIGGLGYFTFTSYAHDK
                     HDEGYQAAKAAAEEEAARTVELIALRGGVPPAGALA"
     assembly_gap    12748..12847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12848..13957
                     /locus_tag="PLD05_01910"
     CDS             <12848..13957
                     /locus_tag="PLD05_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOP76212.1"
                     /translation="PEEEQAAIIAALSAEARLPQQRKIDNEQSAVIARGRELIVRECT
                     RCHEFHGSGPVGQAPVLTGYGSREWLLGLVASPEHVAFYGLRNDRMPVYVEDSLRPEK
                     NRITDVQLSVIVDFIRGDWPRQSEGSQDQDADDTPPVTVAYALGKWDASKKPLASRPT
                     QDPRAEARWLWNKELCGLCHAHTGEGPDNMPAVSSSAPDLGGFASRDWIAGLLDPKQV
                     DSPKYFGNSGFAKGSMVKFVKGNLRELIDEIGKDEFQKLIDALAAEAKKDWKDGEDPP
                     EPDEDTVLLFEDFTCADCHRFYSTGSLGQAPDLTGYGSKRWLAEFIADPKARKFYRQT
                     NDGMPSYHAFPDHPEKNLLTPDEIKILAEYLSPVE"
     gene            complement(14092..14595)
                     /locus_tag="PLD05_01915"
     CDS             complement(14092..14595)
                     /locus_tag="PLD05_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOP76213.1"
                     /translation="MSKQYQLGPIEIPEPPLLRFAEYLRAQGKRMTSQRQIIVNEVFS
                     HHDHFDAEDLINHLRDRLAAREVSRPTIYRTLGELVEAGLLRRMILRERYVYEHQYGY
                     PEHDHLYCQSCHQLFEFHTDALIKIREAVAREYHFTPTSHRMIIMGICADCQRKAEQS
                     SGVSKKA"
     gene            complement(14598..14876)
                     /locus_tag="PLD05_01920"
     CDS             complement(14598..14876)
                     /locus_tag="PLD05_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOP76214.1"
                     /translation="MQVHGPSHIHGAQPLNGPHLSRLGQTSSPSQGAPIRDELHLSPV
                     GQLLDKVHDLPDIRADLVARIRAEIAAGTYETEEKLQVALERLLDEIG"
     gene            15937..16155
                     /locus_tag="PLD05_01925"
     CDS             15937..16155
                     /locus_tag="PLD05_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76215.1"
                     /translation="MNGAKFDCSRKSRESHENNVVPIRVSAEAFRKFQESMKEYWEQV
                     IAQHGSPRRLRIGQRIRRRSRKAPKAKR"
     gene            complement(16219..18393)
                     /locus_tag="PLD05_01930"
     CDS             complement(16219..18393)
                     /locus_tag="PLD05_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOP76216.1"
                     /translation="MRSIQRFLLVMVVGIPGVAMALDWRVLPPGEVPADARQEKLKTL
                     DDYFPFTPPSDLKSWEIRRERVQRQILVAAGLWPMPERPPIQAVIHGAVDRGDYTVEK
                     VYFESYPGFYVTGNLYRPKKGQGPFPGVLCPHGHWKNGRFYDAGLEQVRWDIVNGAER
                     FEVGGRSPLQARCVQLARMGCVVFHYDMVGYADSRQLPHRPGVRQAMNTTERWGYFSP
                     AAELHLQNILGLQTFNSIRALDFLLSLPDVDAKRIGVTGASGGGTQTFLLCAVDPRPT
                     VSFPAVMVSTAMQGGCTCENACYLRIDTGNIEFAALFAPKPLGMTAADDWTREIATKG
                     LPELKQLYTLYGVPNNVMAKPLLHFPHNYNYVSREVMYHWMNKHLSLGLKEPIIEEDY
                     HPLSQEEMTVWDAAHPAPPGGEEFEREFLARLTEATRRQMESLIPDSPEAWQKYANVI
                     GGAYDVMIGRGLPENEDLSVDVRRETQEDSVTARYLLIHQPSRKETVPVLVLVPASPR
                     PTVVIWVSPNGKQGILAEDGKPIPVIRKMLEQKLVVVTADLFGQGETTPDGKNVERQR
                     LVRDDYAGYTFGYNPPLYAQRVHDLLKVIRFARSQLVPEGKLIMIGPQGAAHWVAGAL
                     AQAKGAVDLTVVDTGGFRFQSINAFDHPDFFPGAAKYLDLPGLLSIAAPAKLWLAGEN
                     SEGLSLIRRVYHAAPAEGQLRAWDSLPMDENSEWLKSLIESL"
     gene            complement(18729..>19658)
                     /locus_tag="PLD05_01935"
     CDS             complement(18729..>19658)
                     /locus_tag="PLD05_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOP76217.1"
                     /translation="DDASCLNLYRPQQPIILGVSTTFIERGGFTFTDYLRDENLGNPW
                     ELLKKPPRLLADSRIVIPTILDDATAKYSLHLWGGVGDRLEIQTEDGKRVVYEIVGLL
                     HNSIFQGRLLISEEAFLQVLPDTPGYRFFLVEYLPPPDKDGKSDRETWQSLASIFERI
                     LAELGASFTTTQNRLAALLAVQNTYLSTFQTLGGFGVLLGTVGLGAVVFRGLWERRRE
                     IGLLRAVGFRSRQIVYLVMSENLGLLITGLVIGAGAALVAISPQLATGRATIPYKSLL
                     QMLMAMMAIGTLTAWIATHWLLRQNLIASLRRE"
     assembly_gap    19659..19758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19759..21958)
                     /locus_tag="PLD05_01940"
     CDS             complement(<19759..21958)
                     /locus_tag="PLD05_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76218.1"
                     /translation="MKPITTISGLSYRSLVAYLPSHLAVLLAAATTTAVLTGALILGD
                     SLRFSLRQRAIDRLGEIDWVLRVPHLFRWSWADEVTEIEEFRRCFSHTAIVLHIQTVL
                     EKPGDAPRVVNRVQLFGCDARFWAMGSGQPQHIPRAREIVLNQTLANRLKAKVGDRLT
                     LRIPQLQGIPSESALGRRHDLVRPFAVTVADIVPDVSLGAFSLNLSQEIPCNAYVNLG
                     WLASATGQPGKANLLLLKTANNSSHSSEEAERVLGNLLLPPEDYGVRIERTAKGYVNI
                     TTDRLIFQPNIEEHIVKCLQGLSYERVTVFLAEAVRAGAKEAWYVTMAAMPLAKEPPL
                     GPFLSTEGRVVSQPAVDQCVVNEWLASRLNLRPGDSLEVTWFTPETQRGQVERGKAIL
                     KVADIVSLRGAADDPALVPEVQGLTDRESIADWDPPFPFDASRVTKEDEQYWEQYRAT
                     PKIFVSPEFALKHWKSRFGTTTSIRVVPSAAIDVPQLIQKLTLPLSAIGWTPQPIRSQ
                     ALEAAKGTTPFEWLFLGFSFFVIIAALLLTGLIFALTLERRRVQIGIVRATGWSPRHV
                     LKWLNTEALFLSILAGAVGIIGGVAYAQLMLWALHTVWLPAIGTRFLTLFFRPESLIT
                     GFTFGVLSGLFATLLVIIRLSRRPPSVLLTGQTTDPHSRRAHNRLRLWSLRGVMVCLF
                     LLAALLGIWGTIQGEQNQAGIFFLSGGLILLAFWLTLKLVLYHMDRSRRRI"
     gene            complement(22331..24184)
                     /locus_tag="PLD05_01945"
     CDS             complement(22331..24184)
                     /locus_tag="PLD05_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOP76219.1"
                     /translation="MGTDAVIALAVVGLVFVLLATNRLPADIILMGGLAALILTGVLP
                     SRQALQGFGNEGLVTIAVLFVVAAGLTETGAVNWLTDYLFGRVKSVTLAILRLMVPTA
                     AMSAFVNNTPVVAMLIPAVNDWARQNRVPASKLMLPLSYAAILGGTCTLIGTSTNLIV
                     NGLLIQAHDQQVAAGGHVLPRGLGMFDPTWVGLPATLFGIALVVLAHRWLLPARAVAA
                     PVRQDPREYTTEMLVEPASPLVGKTIEEAGLRHLPGLYLAEINRDGQVLPAVSPQERL
                     RANDRLIFVGIVESVRDLQRIRGLVPATNQVFKLDAPRSDRCLIEAVISDRCPIVGKT
                     IRDGRFRSRYNAVVIGVAREGERLKGKIGDIVLRRGDTVLLEAPPSFVETHRHSRDFY
                     LISPIEHSKPPRYEKAGTALAILIAMVLVVSLSESLGDLTLMLGSLKIGLGRITMLKG
                     ALLAAGAMLAFRCCTLSEVRRAIDWQVLVAIGASIGIGTSLDVTGAAQLVAGYVVDSV
                     QHNPWLALLVIHAMTSLLTELVTNNAAAALMFPFVLATAQQLDVNPMPFIISIITAAS
                     ASFATPLGYQTNLMVYGPGGYRFTDYLRIGVPLNILVTLVTVTIAPLVWPF"
     gene            24815..26434
                     /gene="malQ"
                     /locus_tag="PLD05_01950"
     CDS             24815..26434
                     /gene="malQ"
                     /locus_tag="PLD05_01950"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOP76220.1"
                     /translation="MRESGILLHPSSLPSPMGIGDLGSSAYRFVEWLALAKQRYWQVL
                     PLGPVDDWGSPYSSPSAFAGNELLISLELLAEDGLLDSGELKQQFDHTTDDAPVDYRA
                     VFRKKMAFLKQAFERFEAGRVRNAFDVLREEFELFCEQQSWWLKTYAIYQALVDLLGN
                     NDWPRWPRHVLNLGIDRLRRGGFDALGTDLPGELGKEYRFHCFAQFVFGRQWEQLHRF
                     ALERDVRIFGDIPIYVSYHSADVWANPQYFFLDEHYRPIAVAGVPPDYFNATGQLWGN
                     PVYRWEVIQSEGYRWWIDRFRAVFRLVDLARVDHFRGFAAFWSVPASHETAERGHWVT
                     APGEDLFTRLTTTWEGPWRQLPDGRKILPIVAEDLGHITPDVIALRRKFGFPGMAVLQ
                     FAFGASQVDAFLPDHVAVDTVMYSGTHDNDTSLGWFVHEILPHPQLHERLRRYCAAKA
                     ETISWDMIRLAFESRSQIAIVPLQDVLGLGSEARMNIPGTPDNNWRWRFSFNSLSEAQ
                     AERLARLSEDSGRNTETAILSPVRQASPAKP"
     gene            complement(26438..27556)
                     /gene="mtnA"
                     /locus_tag="PLD05_01955"
     CDS             complement(26438..27556)
                     /gene="mtnA"
                     /locus_tag="PLD05_01955"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOP76221.1"
                     /translation="MNESKPFSTLEWIGDIDGYLKILDQTQLPGKLVYIDCQDEHQVW
                     HAIKSLQVRGAPAIGIAAAYGVCLGLKALASPTEDSLFDRLEAVTAYLASSRPTAVNL
                     FWALDRMKRHAAACRGIMSHQQIRQALLEEARAIHEEDRAICRGIGDWGSTLLSDGMG
                     ILTHCNAGALATSELGTALAAIYTAVASGKRLSVFADETRPLWQGARLTTWELSMHGI
                     PVTLICDNMAAQVMREGKVQAVIVGADRIAANGDTANKIGTYGLAVLAKAHRIPFYVA
                     APSSTFDLSLPHGGLIPIEQRAAEEVTCPFGLPIAPKGVAAYNPAFDVTPAELITAII
                     YERGIISPVNEGTVRAAIEPLHRRKEAISSGEKPDPHR"
     gene            complement(27644..28180)
                     /gene="purE"
                     /locus_tag="PLD05_01960"
     CDS             complement(27644..28180)
                     /gene="purE"
                     /locus_tag="PLD05_01960"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOP76222.1"
                     /translation="MSRPVVGILMGSDSDWQYIKPAASALHEFGVPFEVHVMSAHRTP
                     HQVAEYAAQAETRGLKVILAAAGGAAHLAGVVAAHTTLPVIGIPVPTGLAGGLDSLLS
                     MVQMPGDIPVATVGVGGGGARNAGLLAISILALSDETLAQRLKTFRQRLSEKVKSRDS
                     QLQTKLAEEFGGSGETVG"
     gene            complement(28262..29527)
                     /locus_tag="PLD05_01965"
     CDS             complement(28262..29527)
                     /locus_tag="PLD05_01965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOP76223.1"
                     /translation="MDGWPEVVITGVGIVSPIGIGCEAFWESLCAGRSGITKIEALRE
                     QAWASSIGGVVTNFDAKDRVRPRKALKVMSRDIQMGFVAADLAVEMSGIKSSGVEPER
                     LGIVFGADLIPCELDEIQNAIRRCIVDGHFDFSLWGQYAMSDLYPLWLLKYLPNMPAC
                     HIGIAHDARGPNNTLTLAEVSGLASLIEAVRVIQRGEADVMIAGGTGSRINPSIWVRE
                     GAYELSKRVDDPQHACRPFDAARDGMIHGEGAGAVVLERRDHAVARGARILAQVLGYA
                     CTFEPRRLHHPVQGTAIRQAIRLSLRMADLAPRDVGHVNAHGVSTVDDDRAEAKAIHS
                     ELGDVPVTALKSFFGNLAAGTGIVEMAGSLLAFAGKKIPFTLNYETPDPECPIDVVAK
                     APRPLKIPTAVILNHSRIGQAVSLVLAAG"
     gene            complement(30905..32077)
                     /gene="metK"
                     /locus_tag="PLD05_01970"
     CDS             complement(30905..32077)
                     /gene="metK"
                     /locus_tag="PLD05_01970"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOP76224.1"
                     /translation="MVNGRFLFTSESVSMGHPDKLADQISDGILDALIAEDPTSRVAC
                     ETLVATGMAIVAGEITTKAAINYPDVVRQVIRDVGYTSDSMGFNYETCAVLVSLRRQS
                     PDIAMGVNADTARGKPIGAGDQGMMFGYAINHTPELMPLPISLAHQILQRLTEIRKQG
                     EVDWLRPDSKSQVTVEFDGPVPVRVDTVVVSTQHSDDISQKEIRDFVIEKVVNPILPP
                     ELVNGEIKYYVNPTGRFVTGGPHGDTGVTGRKIIVDTYGGWAKHGGGAFSGKDPTKVD
                     RSAAYMARHIAKNIVAAGLADRCEVQLAYAIGVADPVSIYVDTDGTGKVEERVLCQAI
                     REVFPLTPGGIIEYLDLLRPIYRKTASGGHFGRNLPEFTWEKTDRISDLLDAVGRA"
     gene            32461..33519
                     /locus_tag="PLD05_01975"
     CDS             32461..33519
                     /locus_tag="PLD05_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76225.1"
                     /translation="MVSAAKSSEQGSYTFSRRNMLLASGAVGLGLVAGKSPAFSALLA
                     EGAQGTAQWPVTCRDVMLKRTGEPDCWAAMAAYNVDGVESDITDDLRLPSLYQGDRVF
                     SVAEPQSRQELKAALEAAKKRITAFCMHNRFEERPEFELEWCKKVAVAAKELGVPAIR
                     IDVVPRRMPRTEFLPFAVNLLRKIVDATEDTGVKFAVENHGNTTNDPTFLDALFEGVG
                     SPRLGLTLDTGNFYWFGHPLSRLYEIYERFAHRAYHTHCKSIQYPADMRDKERPMGWE
                     YQKYHCPIDQGDIDFAKVIGILRKAGYSNDLCIENEGLGRLSAEEAVQTVRKEAQLLR
                     NLQSAITQEGRFMPVARA"
     gene            33696..34349
                     /locus_tag="PLD05_01980"
     CDS             33696..34349
                     /locus_tag="PLD05_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76226.1"
                     /translation="MWCNRYQQDIPAFRGPDGQWVCLRCGSGSRSTETGATEAEHLIA
                     QVGSSSSFETPSVSPNEKTESDTPRYDAWELDEELAHLKRLLSIHQVKPGELVTRFDA
                     YHALAKPAHLRKSQVHHDLLRAERLAEAEHGPAWWLFLCSVTIFGCGAILLGWGLALN
                     HERLLIWGIPIFVAGLLVLIASILLPILERRSSRLSPHLPMKRVKTNMETLQAGRDV"
     gene            complement(34386..35465)
                     /locus_tag="PLD05_01985"
     CDS             complement(34386..35465)
                     /locus_tag="PLD05_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76227.1"
                     /translation="MSANQSRSLHPVCWCAAVCLVVIGWVAFSMEPTSRPSRDGRPFT
                     GNEHSRYVHPPHDRVSSGSAGGYFDRHKLRPCGPSVHISQTTVIVSGVGGGVISYQDV
                     VWDTGYPGVPPGYCYYRVPVAYCYPRTVPVYYQPRLIYFDPAAPLLRDLAAVTNRYIG
                     TTRPQPSSSGVAQGSSAAAQAGQVNENRGPSGLAVAEPIKFRPSNEQQRALASRFLTF
                     GDQQFSAGQYRDAYFKYKEAEKAAPDVAESLFRQGFALIATKQYELAFQAFRRGLRID
                     PTWPKKPFSVQRLYHERELDFREHLANLAAECEKKNHDPMLAFLLGVMLFRSGRAEDA
                     ANFFEIARSSPVSADWAALFTNDEP"
     gene            35931..38162
                     /locus_tag="PLD05_01990"
     CDS             35931..38162
                     /locus_tag="PLD05_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOP76228.1"
                     /translation="MMINVFGERDLELATFSTSMRFRYLMRFARTSKLVQVGSTAGRR
                     PSRIVAHYLVTMLTAWLILPVACCSAEDFEWQSADPAEVGISREALDELCRALAERRT
                     KAFFVARHDKVVCEWYSPDHGPDRKHGTASLAKAIVGGMALAVALDDGYVTLDDPVSK
                     YIPQWREDSRKAKITIRHLGSHTSGLDDSRPNEEAPWKDAFWRREDPPNDPFTISRDQ
                     APLKFAPGEGFQYSNPGIAMLTYALAAAMRNAPDNNIRSLLRDRVYRPIGIADNEWSI
                     GYGRTFEVDGLPLVAAWGGGSFTTRATARIGRLVLKEGMWQGKQILSREAIRQVTQSA
                     GLPGDCGMGWWTNAGERFPFLPRDAVWGAGAGDEVLLVIPSLDLIMVRYGNDLGPQVP
                     SQENIGRFLFQPLMKAVLSHSQVSGQTQPPYPPSPVIRELQWAPPEEIIRLARGSDNW
                     PITWGDDDQLYTAYGDGWGFEPRVPKKLSLGLAVVRGMPPTIEGANIRSESVEQYGDG
                     SRGKKASGMLMVDGVLYMLVRNAGNAQLAFSRDRGRSWEWADWKFTVSFGCPVFLNFG
                     PNYAGARDDYVYIYSPDSDSAYTPADRMILARVGKDALLKQEAYEYFVGLDPTGQPRW
                     SRDIHQRGAVFVHAGRCGRSGITYNPALKRYIWYQVYPQSDHPEGPRFSGGCGVFDAP
                     EPWGPWTTVFHTDRWDVGPGESGCFPTKWMSTDGREMWLVFSGEDCFSLRRAKLIVRS
                     ADN"
     gene            38479..>39635
                     /locus_tag="PLD05_01995"
     CDS             38479..>39635
                     /locus_tag="PLD05_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOP76229.1"
                     /translation="MKWWTVKVGVLLTGLSATISNGEPGPGTIRSPYPPSPVFAGIEL
                     DWSTHRREAQGSDLFQLCWADNDHQYGFWGDGGGFGGTNQLGRVSLGFARIEGSWNDY
                     RGFNVWGGKDAETPATFKGKSWGTVCIDGVFYSWIVPDEPDSGGPRDHYRYVELARST
                     DHGATWQKAPWRWYREDELIGPTFLVDGKNHKAAPDSYVYVYFIRPERKDITQPVVGL
                     RVQKPGAIFLARVSRQAIFQGREAYEWFTGIKGNQPVWGHLDQKRPVFENPNGVGWCL
                     SAIYHSGFQRVLLATEHTESSRGCIGLFDAPDPWGPWTTVEYWTADRPFGDQRPGSSW
                     EWKSNIFLISFAPKWFSPDGREFTLVFTGAGNGRDNDSLNTVRGRFRVNANP"
BASE COUNT         8811 a        10709 c        11180 g         8735 t
ORIGIN      
        1 cctactgatc caacgagacg gtggtcttcg atctagcctg accgacttgc ggggggctgt
       61 ccaaatcgct ttaattttca ggatgtttga gaagatcggg tgcacctccc tggccgatga
      121 gaaggtcatt aaaaggtagg ggcgggcccc tgtgcccgcc cgggtaacct tttctatacc
      181 tccccgatat tgaaatgggt agccgcatag tcagggagct tttcgtaccg acccatcagc
      241 cagtgaagtc cgctcttcag tccatcttcg gtggggacgt gccggcggaa acggacgttg
      301 gtcgcgggtc acgcggacag ccgagaggag gatgacccta gaccgcgagc cggaatcttg
      361 atccgtatca tccctggtgc tcgagatcgg gcgtcctgtt tcgaggacta gagacgctcc
      421 aactcaagaa agcaggaacc aaacagctcc aaacatccca atgatcccga gtatcaacac
      481 tggcaagagc gcccacaata gacacagctt catgctccgg cgagaaaagg ctttccatgc
      541 cgccattgag gcgccaatgg ctaggatggg gtaaacgagg cagaggatgg cgaaagcgcg
      601 cggaatgagt atcaaggtaa atggctcgtt acccttcaag ggagcaccaa gcgtcatcgc
      661 ggaggccacc gccgcagccg gataaaccaa caagggtaca agtgccaaga actgaagcaa
      721 caggagaaca agcagcacca gtagcccgcc gaaggtacgc gcggtggaaa gctgttgagg
      781 gttcattgta tctccgctta ttccaaaggt gcgatgtccc gccatccgat tttttgcggc
      841 aagcttgccg tgccctagga agcacctcac ccccgaattc attcccaaaa catggttgcc
      901 ccgacaggac cgccgtcggg ccgaagagtt cgggcgggac tgtctggagc gagtcgatag
      961 tcgccctcgg ccgggcaaac aaaccgcgga tcggcgaaga cagaatgggc atccagaccg
     1021 gttttttgac gatacgtatc gaaatccgga atcttttcgc cgaaccaatg gaccatcacg
     1081 ccggtcgtgc tgaaccaaag gttatagtcc atgtccggaa gtactttcca cccgctcgag
     1141 tagcgactcc cccaatcggt gaagcctgcg aaaatgttgt acttgatttc gatccccttc
     1201 gtggcggcgg tgtttgtata gaacatcagg tgactgccgt tggggtccgg ccgttgggcg
     1261 tgggcccatc ctttgccggc atcgacacat gtgttgttga tgaagcggat gttttccgtg
     1321 acggcctctt cagggttgtt ccaatactcg aatgagtact cagcattgcg gatgaagttg
     1381 ttgcggtagg ttatattcac ttgatgtgaa tctgtcccac gtccttggtt ggtcagcgcg
     1441 gcgtcgtaaa cttcccagat tcggcaaccc tcgaccaggt tgtcgtgggc ggccccccaa
     1501 aactcgatgg cattgccgta acggacgggc tttccgtcgg gccgggtgtg ctggtgtccg
     1561 cccccgatgt acgccagatc gcaattgcgg atgatgatgt gatgcgttcc gccgcctcca
     1621 aagccgtgtg ctgcaccgta catgacagcc aagccgtcgt aaatcacgtg gtgcttaccg
     1681 ctttggttga ccacgtgccg cgtcagcgcc aattcgatgc tgcgatgcgt cgcagtggga
     1741 acgacctcgc taaagaggaa gacccgttgc gaagtcccgt cgtaaaaaaa gtcgtagggt
     1801 ttctcgagcg agtcaagcga ccattttttc caaccacaaa tggcaccatt gtcgaaaatg
     1861 atgtttccca cgtctgtgtc caaagggtca ggcacactgg gcgtagcttc gtggagggtc
     1921 tgagcctgga actcaaagat ggcacccgcg gggatgatcc caccgagatt aatatgcaag
     1981 cccccgtcct cggcagactc gcttgcccgg agcggaattt ggtacgttgc ccactccggc
     2041 ccgactgtct cctttggtaa tgttgattgg gcatagcggg aatatgggaa gccggcttta
     2101 agaagattca ccgggggtag cataaacggt ttgctgcttc tggcgcgaaa ggtaaaaacg
     2161 agatgttcac ccttttggac cgccactgct ggcccccata gttgaatgtg gtttgctgcc
     2221 gagcccgagg cgatgcaggt gacgcgaacg acccggttgt cggcgtcgcc ctcatgcgtc
     2281 aactctacct gggcccctgc ctcttgatgg tgcctccagg tgctattccg caaatcgagt
     2341 cgttcacgtc ccaagcggta ttccacaggc agggtggccc aaagtttggg gccgacgtgg
     2401 acccagtctt ctggccgatt gcggggccgt gacccaagaa gcaggggctt tggcccctcg
     2461 ccgtaagagg tgtaggttac aggggcctgc tcgtcgccgc ttaccgggat gagcgatccc
     2521 cgccacaccc cgccgcactt gaaccggact gtgtcgccgg gtttcaaatc ggcagcgtta
     2581 acgcgctcaa gggatttcca ggcccgttct ggagattggc cgtcggcgtc gtcgtgtccg
     2641 ccctccgagt ccacgaaaaa ggtccgtccg gctcgttctt cggccaagat cgttgcaatg
     2701 tcatggggtg ctacgcaaga aagcaacagc gcgatgactc cgacatgtat aacgtaatac
     2761 ttcatgatct gtttctttct ctgggtgagg gattgaggga atttgtccat ccaacttcct
     2821 tcgggttttc ggcatcacaa acgcgtgtgg atgcttccgg tgtttaagct tagggtgtcc
     2881 ggttcggctt cccgtacaat ggcaagctac acgtcgaaag cctaggaaag acaatttccc
     2941 tgttaccgaa ccagtaacga gactccggcg cacgaaaaca attgaggttc actctaatca
     3001 gttcgttttt ccccgttcca ccagcggggc tagttcctga agtgtccgtt ttgcctccag
     3061 ataccgtcgc tccaggtccg cctgttttaa aagttcacga agccggtttc caatataacg
     3121 ttgggcggtc tccggcgaca tgccttgggg aacggggatc ttttcagggg tctcctccaa
     3181 aatccagttg tcgctccgct gcgtggcaag ggggacgccc cgggaattca cgatgttgcc
     3241 catcggattg cgcgcccaag catatcggta atggaccggc tgaggaacaa atgggctgct
     3301 cagtacgagg atgtggcgct gttctcgcgg ctggttctga ccgtcgtcag acttttcggt
     3361 gtagtacctg acatcggcgg ggaaaaaccg gcgatcttct cccgctatag cgaaacccaa
     3421 aagcttcccg tcggcatcat cctttgtgcg gacttccgtg ctgaaataaa gccgaagcgt
     3481 gccgtcaacg gcttccaccc gggtgatgga aggcggtagc caatactgct cggcgtctcg
     3541 ccccgaaaga atcccgtatt gagtcaccaa tgcccatttt gcagcccgtt ctcccacggg
     3601 aatcttgatc tgaggatggt accaactttt gcggaggtcg taggtgctga cgtagccaat
     3661 ggctttatct ccggcatcgt agaagttgcg gaaggtccgg tactgtgcct cgcggatgta
     3721 gggcccaacg tcgtacatcg cctgaacgaa attttctgca gtttgcggct cgcccgccgt
     3781 ggggagcgcg ataatgcaaa aggccaactg tgggtcgcga aaggcagcac gccaggccgt
     3841 gatcatcctg ccgaagacct ggtaatacat ccgcgctccg gcggacccgt tgaaacaatt
     3901 gttgaatccc tgatgaaaga ggacgccctt gatggaaaag ccttcaaggg ggcggatcac
     3961 gcttgcataa caatagccgg gacggttgcg atcggccgcg gggccaggtc gtaagtcgct
     4021 cggtggggcg ctgttcggag ggatcggctc tccgcgttcc ttccgggcct taatggaggc
     4081 ctcataggca gcgatgcggg cttgcaaatc ggcttgggga tcgtaggcgg caattcgtgc
     4141 gttccagtcc tccagcatct cccgtgtttc gcgaccctca atttgttcca acacttccaa
     4201 aggtgtccac gtctccacgg tggttcctcc aatggatgtg tcgataaggc cgatgggcac
     4261 gcctgtggcc atgtgtaggc ggcggccaaa aacgtaaccg atggccgaaa actctcgcac
     4321 agtctccggc gaacagacat cccagtcgcc tttgcggaag tgtcggttcg accacgaact
     4381 ccactcgtag aggcgttcaa agctccgaac cgaatcaaag ccttttccct gaggtacggt
     4441 caacaggcga atctgaggag aattggccga gacgatctcc agttcgccgt catccacact
     4501 ggcgataggg aattccatat tgctttggcc acccaaaagc cagacatcgc ccaccaagat
     4561 gttttccagc gtgatggtgc gatcggcacc gcggatcgtc atcgtttgcg gtgtcgaatt
     4621 agcggggata ggattcaatg tgacctgcca cgatcgatct tccctcgcct gtgtggtgac
     4681 ctcttggccc gcaaacgaga ccgtcacctt ttcccccgga tttgcccatc cccagatatt
     4741 gatgggctta tcgcgctgga gcaccatgtt ggtttgaaag agattgctca cgcatagccc
     4801 ttcgctgatg gccggaacat cgaccacatc ttcccgcggc atctctcgtg gcctttgctg
     4861 tccatcggct tgcatcagac tgagcagact gaacatcatt cccacgataa caatgattcg
     4921 tttcatagat gcgtccctgt gtctcgaaat gtttgaaccc tcaagcttta cctatcaaag
     4981 aaatgcaccg aaatgtctcc atagtacgga aaacgctgtg tgtctcatca ttctgatcgc
     5041 actttattcg gggcatatct cgattaacga gacggccatg ttgggaagga caacctccag
     5101 cgatgccgtg ccggcatccg tccgaaccag gcttttggat gtgacttcca gcgacgccgt
     5161 ctcgagatta taggccccgc cttgggaatc ctcaccgcgt cgtacgtaac gggtgtaggg
     5221 attattgtgt tcacggtcga tacggtactc cgtgcagcgg aattgttttg cctgcgacat
     5281 ggcgggcggc atttttttca ggcggatttg ccaactccga ggtgagtctt gcatcacgtc
     5341 aaaatgccag aggagtagca caatgcggtt ttcatcagcc gtggccgtca atccgcccgt
     5401 atcatcgtcc aggataccat tacgaccggg caggttgaca cggagctctc cgtgggcaag
     5461 tcggtgatgc attgcccaaa cgaaataggg cgtggtgaca acttgaccct taatcagagg
     5521 cagaccacta taaagggcga tggtctggtc gttgtacacg aacggacggc ctgcccggcg
     5581 ggcaccggat acggtcgtct caaggtgatc catcagtcgc caaatgacct tctccccatg
     5641 atcgctctcc acgacgatgc gggggtctgc ccagaccgcc caatcggctg ttgattggcc
     5701 cttgggcccg ggatcggtgc gaaactcgag gttaatccgc tgtccggcga acgggccgag
     5761 tggcacacgg atttccttcc agggaccggg gcgctgaaat gtctccatca gatgcgtcaa
     5821 cttgtcgcct gaaagggcca ccacctgaaa cccgcagccg tcacttagcg tgggcaactc
     5881 aaccacgccc acagcggcct ccaatcgggg attgccttca gatggtacct gtacctccac
     5941 gcggcagaag gtgtagcgat cctggccgtt agggggatgg agtcgcacgc cgggcaaaga
     6001 gcgccccgcg cacgtatagg ttcccggctg aaagcacacg gggctgtcgg cagttcgcgc
     6061 aaaaggcccc accttttcaa agagtgtcgc ctcttctgcc tgcgtccgca gaaagtgatt
     6121 gaaactgacg atgctggact tcgtgaggcc tgatcgccgc tgatagtgga gcgctgccgc
     6181 caggtaggcc gccccatatt cgttgtcttg ttcgccagtt cgggaccaga ggttccattc
     6241 atcaaggaaa agttcgacgg agggtgcctt ctccggccac acagtttgca cggcactgcg
     6301 gacaccggcg accaggccct catattggcg aggatgggca aaaccatagc cgtggaagca
     6361 aacaaagtcg aggggcacgt ctcgatcacg acaatagcga aggaaatcca cgaacatcgg
     6421 catgggtcgg ccttcgagat cgatcgagcg agcgagggcc ggtccgccca ccttggcctg
     6481 cggatcgagc cgtttcacaa tccgcgaagt ccgctcgtag agttggtaga tggtctccgg
     6541 ctcggcctgc ccgcgggcga ccggaagatc accctcgttc aacgccgtta cccaataacg
     6601 gacgggcagc ttttgctcga aaagcacatg tcggacaata cgttccatca attcatccca
     6661 aagctccaag ttctcgggca aagcgtagcg acggtgttcg gccaaggtgc tcggggacac
     6721 caaggccgac ggggtgtgat acgccaaccg gatgtatggt tcggcccccg cagcagtgat
     6781 cacgctgagc aagcggtcaa agtcgctaaa gtcagtgacc aagcggcctg tctcatcgcg
     6841 ggtcactcgc gtgccccaca gccagccctc agaaaaggcc accaaacggg ccatgcggaa
     6901 gccaccttgc tggaatagac gaaactctgg agagccaacc ccgaatggta ccgggttctc
     6961 ttggctattc caaatggtcg taaaatcgtg aacacgtcta aaaggttgtg tcacggaaca
     7021 atcgacctct gccataactg gcggtttcgg gccggcggca aacggcaaca ctggggcaac
     7081 agggcgaatt tgtagcttgc ctatccaggc ctcggccggt tgcgcactgg ggccacgccg
     7141 cccgatgaaa agctcctcgg gtaacgtcac gagaggaaca gcccggtgcg tcaaggcttc
     7201 ccgcccatcg acgcgcagga cgatccacag cagatctccg taacctttac accagccggc
     7261 tgaaatctcg tgcggctcat tgggttgcca atctgtaatg tccaggtcac aggctgcgtg
     7321 gacctcctcg cgaccacggt aggtaaagcg gagcgtattc gtgtcggtct tgaaaatcgt
     7381 cacatgacag ggaccgatgg ccatgtgtac gaacgtgtga gcacgctgat catttcctgg
     7441 ccaagttggg atgacagtaa acgaaatgct cccttgttct ggtgtcagga gccgctctgc
     7501 cgggaggacg agcatcacct ccgaaccgag aaaaacaaac gattctccac ttatttcttg
     7561 gcttcggact gtccctgcca cgcgaccacc gaaattggtt attgtttgca catcgagtcc
     7621 aatgaacttc gagtcggagg gattaggttg ttcatgccca cgggcctcaa tggacaagga
     7681 tgtccattgc acgacaagcc acagcgcgag gaatcctggc acaatgggga aacaggtttt
     7741 aacaaacgac cttcgcatgt tggaggctcc ttgtaagggg tggagcatca ccctttgcag
     7801 ttgaccgcgc acaagcgtag tggaagtttc ttcaatctca ccgtgccctt tcgaattctc
     7861 acgcacaagc caatgacggc tattatggtc aacgtttcga atatgagaaa tatggcggcc
     7921 gcaaactttt ccacagcagt tgctggcaaa gtaggagcct tgcaagcgtt atcttcggat
     7981 agtaggactt gaaaccgatc gcaccaagcg agcccctgat aatcgtcaga cggacgcgag
     8041 ggcatggccc ctgccagggt cttctcgaaa gacttcagat tgttgcatga ggcaatcgca
     8101 tgacgggagg gtttgggtaa tccgggtatt tgctgttttg attgttctct tgagccccga
     8161 ggtcgtttga tgggcgaaag ggcaggccac attttgcgtt gaatcgaccc ttgcgtctgg
     8221 cacgcacaat ggactgtgaa gaaaaccatg cggcgagatg ccctccccgc attccttggc
     8281 caatctttat aatgatgtgt ttcggttttc gtaaacgaag tgaatcgttg ataatctcga
     8341 gtagcgctgg caacggtagg ctattgtgaa aatattcaga cgcctcgaag aaatcagagg
     8401 tctcgaaggc tcggtcgtca cgatcgggaa tttcgatggt gtccatcggg gacaccttcg
     8461 tctgctggag gtcttgcgaa aggcggccga tgacatgaag ttgccggctg tggtcatgac
     8521 ctttgatccc catcccgccg taattctgcg tggggttgag cctggtccga tcaattgggt
     8581 agaacgcaag ttatccttac tttcgcaggc cggggttgac atcgtcctag cgtatcccac
     8641 agatcgtcgc ctgctggaat tgtccccgga ggccttcttt tatgaggtcc tgcgggatca
     8701 actaggggcg cgggtgattg tcgagggaga agatttccgg ttcggtcgcg accgaacggg
     8761 tgacaccgag atgctgcgac agctttgtga aaaggaaggc atcgggtgca ccgttgtgcc
     8821 gcccgttgtt tggcaagggg caccggtttc cagttctcgg attcgcgagt gtctgatgtg
     8881 cggcgatgtt gtggaagccg cccaattgat gtcgcgacca tttcgaacct gtggagaggt
     8941 ggtgcggggc gcgggccgag gaaggacact tggttttccc accgccaatt tggaaaaagt
     9001 gcgaacgctg ttgccccagc caggtatcta cgcaggtgcc gtgcccctag ggaatcaaat
     9061 ctatcctgcg gccatcagtc tcgggggaaa cccgacgttt gatgaatcac agatgaagct
     9121 ggaagtttat attgtcgatt tcacaggtaa tctttacggt cgggaagtcc aagttgattt
     9181 tcttcagcgt ttgcgtgata tcaagcggtt tgcctcggcc gaggaattga tcgcgcagat
     9241 gtcggacgat gtggagaaga cacgagagat cgtacggcgc tgcgatatga tccagtaggg
     9301 tggagtgtcc taaaaaggga aggggagcag cgcggcagcg caagtagggc accaggggtg
     9361 agaagaaacg gcatcggggt aaaccgtaac acgtttccag aggaggttcg tcgcgtgggt
     9421 tgtgaagatc gtccgaaaat ggctgccgcg ccccattcga tcgattggag ccggctcatt
     9481 aattacttgg aaagtcaccg atcattcgtt ctgaccacgc acattcgacc ggattgcgac
     9541 gccattggaa gtcaattggc cttggccgaa atcttgcgaa atcgcggctg ccaggttgct
     9601 tgtatcaatg ccttcacgat tccgccaccc ctgcagtttt tggataccga gaagcaatta
     9661 atgagggttg accaacccga ggcgagtcgt tgcctcgagc aggccgagat tctcgtgatc
     9721 ctggatacaa cggcctgggc tcagttgggc gatataggac cggtgattcg gcaatttcca
     9781 cgcaagaaaa ttgtcattga tcatcatgtt agcggtgacg atttaggggc cgagttattc
     9841 aagaataccg aagcggaggc caccggccgc atcattgccg aattggcggc tcaatgggga
     9901 gtcgccttga ccccaagact ggcgatgctt ctttttgcgg ccttggcgac agacaccggg
     9961 tggttccgct ttaattctgt gtgcgctgag acgtttcaat tggcagccga cttagcacgg
    10021 gcaggggttc gccctgatgc actgtaccgt cagctttatg agaatgagcg gatcgggcgg
    10081 tttcgcctta tggggcgggc catgtcgcgg gtggaaacag atttggatgg gaaaatcatt
    10141 tactcatata tacggtccag cgatttttca gaaacgggtg ccctaccgtc tgacacggaa
    10201 gacatcatta acacgacact cagcgtcgcg gggactcagg tggccatcat gtttattgag
    10261 catacgcccg gccagttcaa agtgagcctt cggagccggt gccatctgga ttgttgccgt
    10321 ctcgcgggtg agtttggcgg tggtggccac aagtccgcag ccggtgccag tctgaccgga
    10381 tcactcgata gcgtgaaagc ccgtgttctt gacgcggtac ggcgggcgat gcaagaatag
    10441 ggttgcccgc gcgatcgcca tcgcagtgct atcgcaaaca gaggcaccaa gagcaaagat
    10501 gatatgctcg ttcgctgagg acattatttc tgtctgcaaa ccattccccg gcaaagatcg
    10561 tatctgaaca atcgggataa taggcttgcg tttggagaag tggagcggag gtcattgtat
    10621 ggctcagcct gaacgcccga caggtgacgc tcaaccagca tgttgtgcag ctcagaaggc
    10681 ttccgacctg caacagggtg gagagggagg atcgcatgta ccgcggcgcg gcatcctggg
    10741 aagccttgtg gcgtgggtgt tggggctatt cgcactaggt ccctcttttg ttgccgggat
    10801 tgctgcgttt ctttcacccc ttggcgggaa ggcacaaaaa ggggttttgc tccgcttgac
    10861 aacgctggac aatctcccga aagatggaac gccccggaag ttccccgtga ttgcggaaaa
    10921 acgtaatgcg tggacgtttt cgcgcgagcc ggttggggcc gtcttcctcc gccttctccc
    10981 gaacggtgat gtccatgcct tacacgtggt gtgcccccat gcgggatgtg ccatcgaata
    11041 tcggccagca caaagcggtg gccaagaagg gcaatttgtg tgtccatgcc atttggcaca
    11101 ttttgatttg tcgggcaagc ggcttgatgc agtatcgccc agcccacgag acatggacac
    11161 acttcgggtg gaagtccgtg atggcgaagt gtgggtggaa tttcagaact tccaattggg
    11221 cactgcggat aagaaacccg tagcgtgatg cttttgacct ggatatcttg gtcagcaaat
    11281 cgggatcaat gtagacaaga gctgaaagag gaataagagt ctatcccaaa aagtccccct
    11341 ctccctctgg gagagggttg gggtgagggc aagcgatcat cgaagttgat cgagaaaacc
    11401 ggcggagatt cgagccagcc ttacccccga cccctctccc gtcgaacggg gcgaggggag
    11461 attatggggt gggccctaag cggagatgat cacgtcataa ggcgagtttc tcgacggcat
    11521 cagcacttgg aatccaaggg taccgatatg acgtttggcg attggttaga cgatcgaata
    11581 ggctggcgag gaatttggcg ggcaagttgc ggagggggct gcgatcccag gggacgttgc
    11641 tggtggccaa taagtctttc ggttatcttc ttcctcctcg tgcagcagtc gttgacgggc
    11701 ttctttttgt gggtttacta cagtccctca tcgcaatccg cttgggaaag cgtgtatttt
    11761 atacagcatc acatcacgtt gggctggttg ctgcggggga ttcacttttg gggtgcccag
    11821 gtcttggtag gctttctggc gatttcgttg gccctgcgca tcttctcggg attctatcgg
    11881 gccccgcgag agtgggtatt ctggacggga ttggtcctct ttgcgttcgc tctaggatcc
    11941 tgtctgacgg gcgatcttct ccgttgggat caagagggat atgccgccac gcaaacacgg
    12001 gtcagctttc ttatgctgct ccccaaggtc ggggcgagcc tttatcggtt agccgcagga
    12061 ggaccagaat tcggtcatca tacgctaaca cggttttttg ccctccatgt cggagtactc
    12121 ggatttggta tgtggctatt tgctttggcg cacgccgcac tggctcgaag ggcgagtcgt
    12181 gcggtggaaa catcgcctga ggcttataag aacgctcggc cggacccccg gtttcccgtc
    12241 gttgtgcaag gattggtttg tttattggcc ctcgtcgttg tcctcttgtt tgttttccat
    12301 cgtggtcttc ctggcatcgg cgaggtctct gcctggaaat ctccccagac gagttttggg
    12361 gcggccctgg gggcacctgc cgatctcgat ccggctcact tctatggagc agcccgaccc
    12421 gagtggtcgt ttcgtggctt gtatggtttc tccaacgtat ttcctggtga actaaagatt
    12481 ctcccgattt ttgtgatccc cgggatcatt gctgtgttgg tacttgcaat gcccatcctc
    12541 gggaggtgct cattgggcca ctactggaat atgctgctta cagtggtgct gatcggtggc
    12601 ttggggtatt tcacttttac ctcctacgcc catgataagc atgacgaggg ctaccaagca
    12661 gcgaaggcag ccgctgagga agaggccgcg cgaactgtgg aattgattgc tctgcgggga
    12721 ggtgtacccc cggcgggggc gttggccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnncct gaggaggagc aagcggccat cattgcggcc ctttcggcgg aggcacggct
    12901 gccccagcag cggaagatcg ataatgagca gtctgcagta atcgcccggg gaagggagtt
    12961 gattgtccgc gagtgtacgc gatgtcacga attccatgga tcggggcccg tgggacaagc
    13021 ccccgtttta acaggttacg gttctcggga atggcttctc ggccttgttg cctcaccgga
    13081 acatgttgcg ttctatggcc taaggaacga tcggatgcct gtctacgtcg aggacagctt
    13141 acgcccggag aagaatcgca tcacggatgt ccagttgagc gtgatcgttg acttcatccg
    13201 tggcgattgg cctaggcaat cggaagggtc gcaggatcaa gatgctgacg ataccccgcc
    13261 ggtgacggtg gcttacgcct tggggaaatg ggatgcctcg aaaaagccgc tggcatcaag
    13321 gcccacgcag gacccccggg ccgaggcaag atggctttgg aataaggagt tgtgcgggtt
    13381 gtgtcatgcc catacaggcg aaggcccaga taacatgccc gccgttagtt cctcggcacc
    13441 tgatcttggt ggatttgcat cccgtgattg gattgccggc ttgctcgatc ccaaacaggt
    13501 cgattcgccc aagtactttg gcaacagtgg atttgccaaa gggagtatgg tgaagtttgt
    13561 caaaggcaac ctgagagagt tgattgatga aattggaaaa gacgaatttc aaaagttgat
    13621 tgacgctttg gcggcggaag ccaaaaaaga ctggaaggat ggtgaggacc caccagaacc
    13681 tgatgaagat acggttctcc tcttcgaaga ttttacgtgc gcggattgcc atcggtttta
    13741 ttccacggga agtttaggtc aagccccgga cttgaccggt tatggctcca agaggtggtt
    13801 ggcggagttt attgccgatc ccaaggctag gaaattttat cgccagacca acgacgggat
    13861 gccctcctac catgccttcc cggatcatcc cgaaaagaat cttttgacac cagacgagat
    13921 caagattctt gcggagtatt tgtcgcccgt cgagtgaaaa tctctcccag atcgtgtggg
    13981 ttctgtgggg agataatcta agtcccgtga attctccttt gccgacgaat taacgtctcg
    14041 aggaagagat atagcgggcg catggggcgt actcgggcgc aatgcaggta gctacgcttt
    14101 tttgctgaca ccggacgatt gctctgcttt gcgttggcag tcggcgcaga tacccatgat
    14161 gatcatgcgg tgcgacgtgg gggtaaagtg gtactcccga gcgacggctt cccggatctt
    14221 gataagagcg tcggtgtgga actcaaacag ttgatgacac gattggcaat agagatggtc
    14281 gtgttcgggg tagccgtatt gatgctcgta aacgtaccgt tcacgcaaga tcatgcgacg
    14341 caaaagtcca gcctcaacca gttcgcccag tgtccggtaa atggtgggac ggctgacttc
    14401 gcgagcagcg agccggtcac gaagatggtt gatcaggtcc tcagcgtcga agtgatcgtg
    14461 gtggctgaac acttcattga cgatgatttg acgctgagac gtcatccgtt ttccctgcgc
    14521 gcgtaaatat tccgcaaaac ggaggagagg cggttcagga atttcgatcg ggccaagttg
    14581 atactgcttc gacacgccta accgatctca tcgagaagac gttcaagggc cacttgcagt
    14641 ttttcttctg tttcgtacgt accggcggca atctcggcgc ggatccgggc gactaggtcg
    14701 gcgcggatat cagggaggtc gtgcaccttg tccagaagct gccccaccgg ggataaatgc
    14761 aactcatccc gaatgggagc accctggctc ggcgaagagg tctgtcccaa tcgagacaga
    14821 tgcggcccat tcaggggttg agcaccatga atatggctgg gtccatggac ttgcattccc
    14881 ggtctcctct ctcgtcgcgt aacctcagtt gtttcgcaac accgattgcc gatgacctca
    14941 cggcgccgcc cgagaacata tccggcggac gtcttgctcc atactttctc tacaaaacta
    15001 tactccagcc ttgggattta tgccagagcg aataccgaga cttgggcttg attatctttg
    15061 ccatttgcgc cagttgccca ttttatggcg caatatcaga gcattttggc tagatcacaa
    15121 tccgttgaga tgagcctgtc cctggccgcc acacttcgcc ctccacataa acatctttcg
    15181 gctgagtacg cacactatgc tcgcaaaact ctccaagaga cctgtcgtag gaaagatacc
    15241 aaagggacac aaacgacgaa ggcaacgaaa tgcacgccct gttccatttt caaaggttgc
    15301 gccactgagc ctgggatttc gtcttcgcaa caatttctta acgtgcccct cggagcacgg
    15361 taagggaaag ggcttggccg cgaattggta gtactagcgc ttggactctc acggccgctc
    15421 cgaaacctgc ctaaaccact cgggtttatt tcttagatac ctaatggctg cgggccccag
    15481 ccagcttttt tgcacatgac cctaacacca ccagcccgtg gtgtggcgcc cggcgggatt
    15541 gttcccactg ggcgtttatt ttattgaaat gcttatctat caagtagtat cttgctgttt
    15601 tgtgagattc ttggtcgctt ggagatcttc ttggcggcga ggtcagttct tggggaaact
    15661 tgcggcttgt tggcttctcc gggtttgcag cgaaccagaa tctcgcccgg cacgtccaag
    15721 agttagaagg ccagaaacca ggttatctgg gactggcgaa ccacagggac cggctgggga
    15781 ataggttttt ccttcgagtg tcaacagttt gtggggcgat ccacaaatca gagggttcta
    15841 gaaaatcgtt ttcacattcc tttcctttgg cgtccctcgt ggataggatg gaaacaagtg
    15901 agacgagaat cggggcctta attttggggt gaactaatga acggtgcgaa attcgattgc
    15961 agcagaaaaa gtcgggaatc gcacgaaaac aacgtcgtgc cgatccgcgt gtcggccgag
    16021 gcttttcgga agtttcaaga gtcaatgaag gaatactggg aacaggttat cgcccaacat
    16081 gggtcaccgc gtcgcttgcg gattgggcag cgaattcgcc gccgttcgcg gaaggcccca
    16141 aaagctaagc gataagcgtt tatccggctg tatgcctttt tgtgcactta tcctattttc
    16201 tctaaattgc ccaaagcctt agagggattc tatcaagctc ttgagccatt cggagttttc
    16261 atccattgga agtgaatccc aagcccgcaa ctgtccttcg gccggtgcag catgatagac
    16321 ccttcgaata agggagagcc cttcgctatt ctccccagct agccacaact tcgccggtgc
    16381 cgcaattgac agcaatccgg ggagatcgag gtatttggcc gccccgggga aaaagtcggg
    16441 atggtcgaag gcgttgatcg attgaaaccg gaacccaccg gtatctacca ccgttagatc
    16501 aacggcaccc ttggcttggg ctagggcacc cgccacccaa tgagcggcac cctgggggcc
    16561 aatcataatg agttttccct cgggaaccaa ttgcgagcgg gcaaaccgga tgactttgag
    16621 caagtcgtga acccgctggg catacagggg cggattgtac ccaaacgtgt agcccgcgta
    16681 gtcatcccgc acgagacgtt gccgctcgac attcttgccg tccggggtag tttcgccttg
    16741 gccaaaaagg tccgctgtga ccacaacaag tttttgctct aacatctttc ggatgacggg
    16801 aataggcttg ccatcctcgg caaggatgcc ttgcttgccg ttgggactga cccagatcac
    16861 gaccgtcggt cggggcgatg caggaactag gaccaagact ggcacagttt cctttcttga
    16921 cggttgatgg atcaaaaggt accgggcggt aacactatcc tcctgcgttt cccgccggac
    16981 gtccacgctc aggtcttcgt tctctggcaa cccgcggccg atcattacgt cgtaagcccc
    17041 tcctatcacg ttggcatact tttgccaggc ctcgggagaa tcaggaatga gcgattccat
    17101 ctgtcgccga gttgcctcgg tgagacgtgc cagaaactct cgctcaaact cctctccgcc
    17161 aggtggcgct gggtgcgctg cgtcccagac tgtcatctct tcctggctaa ggggatggta
    17221 gtcttcctcg atgataggct ccttaaggcc cagcgacagg tgcttattca tccagtggta
    17281 catgacttcg cgcgaaacat agttgtagtt gtgcggaaag tggagaaggg gcttggccat
    17341 cacattgttg ggaacgccgt acaacgtgta aagctgctta agttcgggta accctttggt
    17401 tgcgatttcc ctagtccagt cgtccgcggc cgtcatcccg aggggttttg gggcaaagag
    17461 ggctgcaaac tcgatattgc cggtatcaat ccgcagatag caagcgtttt cgcacgtgca
    17521 gccgccttgc atggccgtag agaccatcac cgcggggaac gagacggtgg gacgggggtc
    17581 aacagcacac agcaagaagg tttgcgtgcc tccgccactg gcccccgtca ctccgatccg
    17641 ttttgcgtcc acgtctggca agctgaggag aaaatccaaa gcccgaatgg agttgaacgt
    17701 ttgcagtccc aatatgtttt gcaggtgcaa ctccgctgcc gggctaaaat agccccagcg
    17761 ctccgtcgtg ttcatggctt gccggacgcc ggggcgatgg ggcagttgtc ggctgtcggc
    17821 gtagccaacc atgtcgtaat ggaatacgac gcaccccatc cgtgctaact gaacgcacct
    17881 cgcttgaagt ggagatcgac caccgacttc aaagcgttcc gccccgttca cgatatccca
    17941 gcggacttgt tccaatcccg cgtcataaaa gcgaccgttt ttccagtgac cgtgcgggca
    18001 aagaacaccc gggaaaggtc cctggccctt ctttgggcgg taaagattcc cggtgacgta
    18061 aaaccctgga tagctctcga aataaacctt ctccacggtg taatcgccgc gatcaacagc
    18121 accatggatg accgcctgga tgggaggtcg ttccggcatc ggccaaagcc ctgcagccac
    18181 caggatttgc cgctgaacac gttcgcgccg aatctcccag gatttgagat cggaaggtgg
    18241 ggtaaacggg aaatagtcgt cgagcgtctt cagtttttcc tggcgggcat cggcggggac
    18301 ctctccgggc ggcaacacac gccaatcgag ggccatcgcc acgccgggaa tcccgaccac
    18361 catgaccaaa agaaatcgct gaatactccg catgaccatg acctccgcag gcagaagaca
    18421 acccgatcgt taccgagctt ttcctaagat caccgtgctg gcggatttta taagccactt
    18481 tcgcccggtt ttcaatcccg cgtggttctg agcgatctag gattcacccg gatttcaggc
    18541 acgtgcgcga gatttggtgt atccttgagc ggattcgctg agggtttctc cttcgtgtga
    18601 agtggggtct tctaatgggg gatagattcc atgccggtcg cggctggccg agagaaagac
    18661 cttgcctgag gagaatcgta tcatcagaca aagtcagaga aactcttcgg aattgtgtga
    18721 ctagaggttc actcgcgtcg cagactggcg atcaggttct ggcggagcaa ccaatgggta
    18781 gctatccacg ccgtcagtgt gccaatcgcc atcatcgcca ttaacatttg aaggagtgat
    18841 ttgtaaggga tggttgcacg tcccgtggcc aactgcggcg agatcgccac gagcgccgca
    18901 ccggcaccaa taacgagccc cgtgatcaac aatccaaggt tttctgacat cacaagatat
    18961 acgatttgcc gcgaccggaa cccgactgcc cgaaggagac cgatttcgcg gcgtcgttcc
    19021 cacaatcccc gaaagacgac cgcacccaag ccgaccgtcc caaggagcac accaaatcca
    19081 ccaagcgttt ggaacgtgga gagataggta ttctggacgg ctaacaaggc ggccaaacga
    19141 ttttgagtcg tcgtaaagct ggcccccagc tcagcaagaa tccgctcaaa gattgaagcc
    19201 agtgactgcc aggtttcgcg gtcgcttttt ccgtccttgt cgggcggggg taggtactct
    19261 acgagaaaaa accgataacc cggggtatcg ggcagcactt ggaggaatgc ctcctcggaa
    19321 atgagtaacc ttccctgaaa gatgctgttg tgcagtagcc ccacgatttc atagaccacc
    19381 cgcttgccat cctccgtctg gatttccaaa cgatcgccca cccctcccca aagatgcaag
    19441 gagtattttg ccgtggcatc gtcgaggatt gtcgggatca cgatacggct gtccgccaga
    19501 agtcgtggag gttttttcaa gagctcccag ggattgccca agttctcatc tcgaagatag
    19561 tcggtaaacg taaaaccacc ccgttcgatg aatgtcgtac tgacgccgag gatgatgggc
    19621 tgttgcgggc ggtacaaatt gaggcaactg gcgtcatcnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnngg attcttcttc gcgaccggtc catgtgatag aggacgagct
    19801 ttagggtgag ccaaaacgcc agtaatatga ggccaccgct aagaaagaag ataccggcct
    19861 gattctgttc tccctggatc gttccccaaa tgcccaatag tgctgcaagc aggaagaggc
    19921 aaaccatgac gccacgcaga ctccataatc gaaggcgatt gtgggcacga cgcgagtgtg
    19981 ggtccgtcgt ctgaccggtc aggagcacac tgggtggcct ccgtgataag cgtatgatga
    20041 ccaaaagcgt ggcgaaaaga ccgctgagca cgccaaaagt gaaaccggtg atcaaggatt
    20101 ccggacggaa aaagagggtt aaaaagcgtg tcccaatggc aggaagccaa acggtgtgta
    20161 acgcccaaag cataagttgg gcataggcaa caccgccaat gattccgact gccccggcaa
    20221 ggatgcttag aaaaagggcc tccgtattaa gccacttcag aacatgccga ggggaccaac
    20281 ctgttgcccg aacaataccg atctgcacgc gccttctctc gagcgtgagg gcaaaaataa
    20341 ggcccgttaa gagcaaggcg gctatgatga caaaaaagct gaaaccgaga aaaagccact
    20401 caaagggtgt cgttcctttg gccgcctcaa gtgcctgcga ccggatcggt tggggtgtcc
    20461 agccaattgc cgagagtggg agcgtcaatt tttggatgag ttgtggcaca tctatcgctg
    20521 ccgaaggtac cacacgaatg gacgttgtag tgccaaagcg gcttttccaa tgcttcaaag
    20581 cgaactcggg tgacacaaag atcttaggtg ttgcccgata ttgttcccag tattgctcat
    20641 cttccttcgt aacccgcgac gcatcaaatg ggaaaggagg gtcccagtcg gcaatcgact
    20701 cgcgatcggt caaaccttga acctccggca ccaaagccgg atcatctgcg gcccctcgga
    20761 gagagacaat atcggccacc ttcagaattg ccttaccgcg ttcgacttga ccgcgttgag
    20821 tttcaggagt gaaccacgtt acctcaaggg aatcaccggg ccgaaggttt aatcgcgacg
    20881 cgagccactc gttcacgacg cattggtcaa cagcgggttg agaaacgaca cgaccctcgg
    20941 ttgaaagaaa cggcccaagt ggcggctcct tcgccaaagg catcgcagcc atcgtgacgt
    21001 accaagcctc cttcgccccg gcgcggaccg cttctgccag aaaaactgtt acccgctcat
    21061 acgagagacc ttgcaggcat ttaacaatgt gctcttcaat gttcggctgg aaaataagac
    21121 ggtcggtggt gatgttcacg taccccttgg ccgtgcgttc gatccgcacc ccgtagtctt
    21181 ccggtggtag taaaaggttc cctaacacac gttctgcctc ttccgaagaa tggcttgaat
    21241 tgttagcggt cttcagaaga aggaggttgg ccttgccagg ttggcctgtg gcggaggcca
    21301 accaccccaa gttgacgtag gcattgcaag gaatctcttg agacaagttc aggctgaagg
    21361 caccaagact tacatcgggc acgatatcag ccacggtgac cgcaaaaggg cgcacaaggt
    21421 cgtgccgcct gcccagggca ctttccgagg ggattccttg gagttgagga atcctcaatg
    21481 tcaaacgatc gccgaccttg gccttcaggc gattggccaa tgtttgatta aggacgattt
    21541 ctcgcgccct gggaatgtgt tgaggttgtc cagaccccat cgcccaaaat cgagcatcgc
    21601 atccgaaaag ttgcacccga ttcaccacac gcggcgcgtc gccgggtttt tccaacacgg
    21661 tctgaatatg caagacgata gccgtatgag aaaaacagcg acggaattcc tcaatctcgg
    21721 tgacctcgtc cgcccacgac caccggaata aatgagggac acgcaagacc caatcaattt
    21781 caccgaggcg atcgatcgcg cgttgacgca gactaaagcg gagggaatca ccgaggatca
    21841 gtgccccggt caaaaccgcc gtggttgtgg ccgcagcaag taacaccgcc agatggctag
    21901 gaagatacgc caccaacgag cgataggaca gacctgagat ggtcgttatg ggtttcatgg
    21961 accgaaggca ctccatactc cgaaacgtta atcattataa ggctttgcct ctggccatcc
    22021 gaccacgggt tgatcatcag gagaagaact aacagcgaat ggaaaaaacg gcatttcaca
    22081 cgggcctttg ctgttacgat ggtgcgattg atgtactccg acggtgtcac cacgaagtcg
    22141 cggcgacact cgtgtttgac actctttggc caatggctga gatttgccaa gattggacat
    22201 gaccttcgcg ccattgactc cacaacaaag gaagtcggct gcaaattgtg gctgcgggtg
    22261 ttagcaaggg gcgtggtcgc gtgacaacac gccccaccaa gtcaggtcac cggaactggt
    22321 agcggtctag ctagaatggc cacacgagcg gcgcaatcgt aactgtgacc agggtcacaa
    22381 gaatgtttaa ggggacaccg atccgcaggt aatcggtaaa acgatagcct ccgggtccat
    22441 agaccatcaa gttggtttga taacccaggg gtgtggcaaa tgatgctgag gccgccgtga
    22501 tgatcgaaat gataaaaggc atggggttaa cgtccaactg ttgggctgtt gccaaaacga
    22561 aaggaaacat taaggcagcc gccgcattat tggtcacgag ctcggtcagc aaggacgtca
    22621 tggcgtggat gacgagtagt gccaaccatg gattgtgttg cacagagtcc acgacgtaac
    22681 cggccactaa ctgggcggca cctgtaacat ctagtgaggt tcctatcccg atggaggcac
    22741 caattgccac taggacctgc caatcgatgg ctcggcggac ctcgctcagg gtgcagcagc
    22801 ggaaggcaag catggccccc gcggccagta gggcaccctt taacatcgtg atgcgtccca
    22861 ggccaatttt gagcgaacct aacatgaggg ttaaatcgcc cagagactca ctaaggctga
    22921 ccacaagcac catggcgata aggatggcca gggccgtccc cgctttttca tatcggggcg
    22981 gtttcgagtg ctcaatgggg ctgatgagat aaaaatcccg gctatgccga tgggtttcca
    23041 caaaggacgg aggggcttct agaaggacgg tatctccgcg tcgcaggaca atatcaccga
    23101 tctttccttt taggcgttct ccctcacgtg ctacaccaat aacaacggcg ttgtaacgtg
    23161 atcgaaaacg accgtcgcga attgtctttc ccacaatcgg gcagcgatca ctaatcacgg
    23221 cctcgataag acaacgatca gagcgcgggg cgtccagctt aaaaacttga tttgttgcgg
    23281 ggacaagccc gcggatgcgt tgcaaatccc tcacggattc gacaattccc acaaagataa
    23341 gacgatcatt ggcccgaagc cgctcctggg gcgaaaccgc cggcagcacc tgaccatcac
    23401 gattgatttc cgccagatac aggccgggca aatgacgaag tcctgcctcc tcgatcgttt
    23461 tccccacaag aggacttgcc ggctcgacaa gcatctcggt ggtgtactct cgcggatcct
    23521 gtcggaccgg agcagccaca gccctagcag gcagtaacca acgatgagcc aaaacaacca
    23581 aggcgattcc aaaaagggtc gccggcaatc caacccatgt gggatcaaac atccctaacc
    23641 cccgtggcag aacatgccct cctgcggcga cttgttggtc atgcgcctgg ataagcagtc
    23701 cgttgacgat tagattggta ctggtgccaa tcagggtgca tgtaccgcca agaatcgccg
    23761 cataactgag gggcagcatg agcttcgatg cgggaactct gttctggcga gcccaatcgt
    23821 tcactgccgg aatgagcatg gccacgacgg gggtattgtt cacaaacgcg ctcatcgcgg
    23881 ccgtgggtac catgagcctg agaatcgcta gggtgactga ctttactcgg ccaaataggt
    23941 agtctgtcag ccaattcaca gcccctgttt ccgtaaggcc cgccgccacc acaaagagca
    24001 cggcaattgt cacgaggcct tcgttcccga aaccctgaag ggcctgacgg cttggtaaga
    24061 cacccgtgag aatcagtgcc gccagccccc ccatgaggat aatatctgcc ggcaagcgat
    24121 tcgttgccag caagacgaaa actaaaccga cgacagctaa cgcaataacg gcatcagtgc
    24181 ccatagacgt tccccagtgg ttttattcgt ctcaggatac aaaagcatag gacggaagca
    24241 aaatcctttc acaggggctt gttacccgtc agctttcagc gtcctgtctt atttcggttt
    24301 ggaccgcacg acggcggccc catttgacat ctagagacag gctccggcga gccgtaaatc
    24361 accaaagtga cgacggtgta tttctaaatc gaggcaaaca tgatcttttc tatcatgata
    24421 gtatactttc catttttagg gtagtccgtt ggggcctccg ctccttagag gacgttttgc
    24481 ctgaaagtct cagcttctcg actctcgatc atggagccga caaagccggt taaaatccgt
    24541 gatctgactc ccgctccttc ctgttatacc tttcctaaat tccctattgt tgtcatcctg
    24601 caagctgatg cccaaattag gggctcgtat gtggttcaaa gccccctgtg tctcggtccc
    24661 gcatcccata gaatgagtta tggacgaaat ccaggaaaac cgctataagg gttgatttct
    24721 tctggtccat ccctcctgtc aagttcgtgg ggatgcgtgg gtgtgttgaa gcgccattgc
    24781 gattgcattc catttacggt gttgatttct tcaaatgcgt gaaagcggaa tcttacttca
    24841 tccgagctca cttcccagcc caatggggat tggtgatctt ggttcttctg catatcgctt
    24901 tgtcgagtgg cttgctctgg caaaacagcg atattggcag gtccttcccc tgggacccgt
    24961 ggacgattgg gggtcaccat atagttctcc ctcagcgttt gctgggaacg aactgttaat
    25021 ctcgctggaa ctccttgccg aagacggact cctcgatagc ggagaactaa aacaacaatt
    25081 tgatcacaca acggatgatg cgccggtcga ttatcgagca gtgtttcgca agaagatggc
    25141 attcttgaaa caagccttcg aaagatttga ggccggccgc gtcagaaacg cattcgatgt
    25201 actccgggaa gaatttgagc ttttctgcga gcaacagtct tggtggctca agacgtacgc
    25261 gatctatcaa gcattggtgg accttctcgg aaacaacgat tggccgcgtt ggccgcgaca
    25321 cgtgctcaac ctaggtatcg atcggcttcg acgtggcggg ttcgatgctc taggtacgga
    25381 tcttcctggc gagttgggga aagagtatcg ctttcattgt tttgcccagt ttgttttcgg
    25441 ccgtcagtgg gaacaattac accggtttgc gctcgagcgg gacgtccgga tttttggaga
    25501 tatcccaatc tatgtctcct atcacagcgc cgacgtctgg gccaatcccc agtatttttt
    25561 tcttgacgaa cattaccgac cgatagcggt tgccggggta ccgccggatt acttcaatgc
    25621 cacgggccaa ctttggggaa atcccgttta ccgatgggag gtgattcaaa gtgagggcta
    25681 ccggtggtgg atcgatcggt ttcgcgctgt gttccgcctg gttgaccttg cccgggtcga
    25741 tcattttcgt gggtttgccg ccttttggtc ggttcccgct tcgcacgaaa cggccgagcg
    25801 gggacactgg gtgaccgcac cgggtgagga cctctttacc cggcttacca cgacatggga
    25861 ggggccgtgg cgacagttgc ctgacggtcg gaaaatcctc cctattgtgg cggaggacct
    25921 cggccatatt accccagacg taattgcctt gcgacgcaaa tttggtttcc cgggcatggc
    25981 cgtgctccaa ttcgcctttg gggcatccca agtcgatgct ttcttgcctg accatgtggc
    26041 tgtggatacc gtgatgtact ccggtacaca tgacaatgat acaagccttg gttggtttgt
    26101 tcacgaaatc ttgccgcatc cccagttgca cgagcgactt cgacggtact gtgcagccaa
    26161 ggcggaaacc attagttggg acatgatccg actggcgttc gaatctcgtt ctcagattgc
    26221 cattgtcccg cttcaggatg tccttggctt aggttcggag gcgcggatga atattccggg
    26281 aacaccggat aataactggc ggtggcggtt ttcttttaat tctctaagtg aagcacaggc
    26341 cgaacgattg gcccgacttt cggaagactc ggggcggaat acagagaccg cgattctatc
    26401 tcctgttagg caagcatcac cggcgaagcc gtgatcctca ccggtgagga tcaggtttct
    26461 cgccgcttga gatcgcctct ttgcggcgat gaaggggctc aatcgccgcc cgaaccgtcc
    26521 cctcgttgac cggtgaaata attcctcgtt cgtaaatgat tgccgtgatt aactctgcgg
    26581 gcgtcacgtc aaaggcggga ttataagcag caacgccctt tggagctatt ggtaggccaa
    26641 aagggcaagt cacttcttcc gctgctcgtt gttcaatggg gatcaaaccg ccgtgaggca
    26701 agctgaggtc aaacgtggaa cttggggcgg ccacgtaaaa gggaatccga tgggccttgg
    26761 ccaacaccgc cagaccatag gttccaatct tgttggcggt atcaccattg gcagcgattc
    26821 ggtcggctcc aacaatgacc gcttggactt ttccctcccg catgacctga gccgccatgt
    26881 tatcgcaaat aagcgtcacg ggaatgccat gcatgctcag ttcccaggtt gtcagccgtg
    26941 caccctgcca cagcggacga gtttcatccg cgaacaccga caaccttttt cccgaggcaa
    27001 cggccgtata gatggcggcc aaggccgtcc ccagttcgga tgtggccagc gcaccggcgt
    27061 tgcagtgggt caaaatcccc attccgtccg acagaagtgt gctcccccaa tcgccgatac
    27121 cgcggcaaat cgcccggtct tcctcgtgaa tggccctagc ctcttcaaga agggcttggc
    27181 gtatctgctg atgcgacatg attcctcggc aggccgcggc atggcgcttc atgcggtcta
    27241 aagcccagaa caaattgacc gcggttggtc gggaagacgc aaggtaagct gtgacggcct
    27301 ctaatcggtc gaaaaggctg tcctcagtgg gggaggccaa agctttcaat ccgagacata
    27361 ccccataagc cgcggcgatc ccaatggccg gggccccccg aacttgcaga gacttgatcg
    27421 catgccagac ctggtgttcg tcctgacagt cgatgtacac caacttgccg ggtaactgtg
    27481 tctgatcgag aatcttcaga tacccatcga tatcgccaat ccactcgagt gtggaaaacg
    27541 gtttcgattc gttcattgcc aagatcttct gaccaaaatg gtaatatatt gaggtgaagc
    27601 acctaattga cggctcaggg cggtcgaact ctgcgaagag aacctaacca acggtttccc
    27661 cgctaccacc aaactcttct gcaagtttcg tttgcaactg gctatcccgc gacttgactt
    27721 tttcgctgag ccgctgcctg aaggtcttaa gccgttgggc caatgtctcg tcactgagag
    27781 ccaagattga aatcgccaga aggccggcgt tgcgtgcgcc accgccacct actccaacgg
    27841 ttgccaccgg gatgtcgccc ggcatctgga ccatggacaa cagcgagtcc aatccccccg
    27901 ccaagcccgt gggcacgggg atcccaatga ccggcaaggt cgtatgagcc gcgacaacac
    27961 cggccagatg ggcagctccc cccgcagccg ccagaatgac cttcagaccg cgagtttctg
    28021 cctgggctgc gtactccgca acttggtgtg gcgtgcgatg cgcgctcatc acgtgaactt
    28081 caaagggaac cccaaattcg tgcaaagcac tcgccgctgg cttgatatac tgccagtcac
    28141 tgtcgctacc cataaggatg ccaacaaccg gtcgggacat agtttttcac tctcctcact
    28201 tgcttggctg agtctttttc gggttctgga tcaaaatcgc aggccaatca gtttttcagg
    28261 atcagccggc ggcgagcact aacgagactg cctgtccgat ccgtgagtga ttgaggatca
    28321 cggccgtggg gattttcaac ggtctgggtg ccttagccac gacgtcaatc ggacactctg
    28381 gatcaggcgt ttcgtagttc agggtgaagg gtatcttctt tcccgcaaag gccaaaagac
    28441 tgcccgccat ttccacgatg cctgttccgg cggccaaatt tccaaagaag ctctttaatg
    28501 ccgtcacagg gacatcaccg agttcgctgt ggatcgcctt ggcctcggcc ctgtcgtcgt
    28561 ccacggtgct aactccgtgg gcattgacgt gtcccacgtc acgaggcgcc aaatcagcca
    28621 ttcttaacga taaccgaatt gcctgccgga tggccgtacc ctggacaggg tgatgaagcc
    28681 gtcgcggctc aaacgtacag gcgtagccga gaacttgcgc caggatcctg gcccctcggg
    28741 ctacggcatg gtcacggcgt tcgaggacca ctgcaccagc cccttcgccg tggatcatgc
    28801 cgtcgcgagc tgcatcgaac ggacggcagg catgctgcgg atcatcaact cgttttgaca
    28861 actcgtaggc cccttcgcgg acccaaatgg aaggattgat cctcgaccca gtcccgcccg
    28921 caatcatgac atcggcttca ccccgctgga tgacccgcac cgcttcgatc agcgaggcca
    28981 ggcccgagac ctcggcgagc gtgagtgtat tgtttggccc ccgcgcatcg tgggcaatgc
    29041 caatatgaca cgccggcata ttgggcaaat attttaggag ccatagagga taaagatcgg
    29101 acatcgcgta ctggccccac agggagaagt caaagtgccc atccacgata cagcggcgaa
    29161 tcgcgttttg aatctcgtcc aattcgcacg ggatgagatc ggcgccaaaa acaatgccca
    29221 gtcgctcggg ctccacacca ctcgacttga tccccgacat ctcgaccgcc agatccgccg
    29281 cgacgaatcc catttggatg tcgcgactca taaccttgag ggcttttcgg ggacggacgc
    29341 ggtctttagc atcaaaattg gtaacaaccc cgccaatgct ggatgcccaa gcttgctctc
    29401 gaagggcctc aattttggtg atgcctgatc gccccgcgca aagagactcc cagaaggcct
    29461 cacagccgat gccaatagga ctgacaatac caacaccggt tatgacgact tcaggccaac
    29521 cgtccatgct gtgcttgtta tgcggcacct gccatccgcg aacgtgttat gaaatactac
    29581 ccaggtcgtt ctgtcaccgg acacgcttgc ccatgcccat atgacgacgg tttttcggtc
    29641 taaaatcgtg atactaccca agaattgggt taacgacaag gtaaacaatt ttccgcggaa
    29701 aagctgattc acgcgaggag ttttgagatc ccgggccaaa atagcccata aacccaaccg
    29761 ggttaggcgt tctcttttgc acaaaaacgc acgggaatac ctgttctgta cagtggcgga
    29821 gaaggcaacc gaacgtccaa tcgcatccca aagggataaa tcttaccgaa ctggaccctt
    29881 gtcttggcac cgtgccaaac ccgaaccttt gggacgctct cgaatggtgg ccaaaacgga
    29941 aagtggacac tgacgacctg ctgctgcggc cgaaaggcca cccagctttg ccccaagacc
    30001 actcgatgcc ccttttcatc gcaaaattcg gcgatgcgga gtattggctt tcggacttgt
    30061 cggcttcggt atctggacct tcgcgaagat gtgcgttcct gccgaacggt tgacaaaaga
    30121 catccacggg gaagtgcccc aaggcgaggg gaatccgatg gcttgaatcc aaggctctct
    30181 agaaagactg cgcttgttgg ccaaataggt ctagtagcat acgaggggac aagacccgat
    30241 ggatagcggc aagacctatc acctctgtct ggtaatatgt gatattcggg caatttgggg
    30301 aaatttgcca ggattgcacc cagactgctt atcagcaatg ccgtaataat gaagcctgac
    30361 gaatgctctt gggctaacaa tgcgagaacg ctcccgaccg tgaaaaccgc gccaaaggca
    30421 accaggacca agggatgttg gaccaacctg tgtgtgatgc gttggacgac cactcctctt
    30481 cccctaatca agtacgctag aaccaagggg aggaagacaa gcagaaccac ggcgcgcaag
    30541 acaaccgact ccgccctcgt caaaacgagg actgccacga taacggccac cacccagcag
    30601 cagatgccca aaaccaacac cagccatcgc gtgacagtag cgatggggtc ccgagagttg
    30661 gatctgctgg ggtaaacact gaccatgaat gggcgaaatc cggcctaact tccttggcgt
    30721 cggccagcga aaacttattc ggttaagagc ctatctcaaa aggtcgccct ctccctccgg
    30781 gagagggttg gggtgagggc aaccgaacat cggaatcaat tgagaaaacc ggcaacgatt
    30841 cgagccaccc tcacccccga cccctctccc gccgaacggg cgaggggaga tcatgggata
    30901 ggctctaagc acgacccact gcgtcaagca gatcggagat gcgatcggtc ttttcccatg
    30961 tgaattcagg gagatttcga ccgaaatgac cacccgatgc cgtcttccga taaatgggac
    31021 gtagcagatc gagatactcg ataatcccgc cgggcgtcaa cgggaacacc tcacgaatgg
    31081 cttggcacaa gactctctct tcgaccttac cggtcccatc ggtgtccaca taaatgctga
    31141 cgggatcggc gacgccgatg gcgtacgcca gttggacctc acaacgatcg gccaagcccg
    31201 ccgccacgat gttcttggcg atgtgtcggg ccatgtatgc ggcgctgcgg tcaacctttg
    31261 ttgggtcttt cccgctgaag gccccgccgc catgctttgc ccagccgcca taggtgtcca
    31321 cgatgatttt tcggccagtc acacctgtat cgccatgcgg cccaccggtg acgaacctcc
    31381 ccgtggggtt gacgtaatac ttgatttcac cattgactag ttcgggcgga aggatcggat
    31441 tgaccacctt ctcaatcaca aaatcgcgaa tctctttttg gctaatatca tcactgtgtt
    31501 gcgttgacac cacgacggta tccacccgaa cgggcaccgg gccatcaaac tcgacggtca
    31561 cttgactctt gctgtccggg cgaagccaat cgacctcgcc ctgctttcga atttccgtca
    31621 agcgttgaag gatttgatgg gccaaggaaa tgggcaaggg catcaactcg ggggtgtggt
    31681 tgattgcgta gccgaacatc atcccctggt cgccggcccc gatgggcttc ccgcgagccg
    31741 tgtcggcatt cacacccatg gcgatgtcag ggctctgccg ccgcagcgag acgagcacag
    31801 cgcaggtctc atagttaaag cccatgctgt cgctggtgta tccgacatca cgaatgacct
    31861 ggcgaaccac atcggggtaa ttgatggccg ccttcgtggt aatctctcca gccacaatcg
    31921 ccatgcctgt ggcaaccagg gtctcgcagg ctacccggct ggtgggatct tccgcgatga
    31981 gagcatccaa aatgccatcc gaaatctgat ctgccaactt gtccggatgg cccatgctga
    32041 ctgactcact tgtaaacaag aaccgaccat taaccacgga gactgcctcc tgaattaaaa
    32101 cgcctcagaa agaatctttt caactgagtc ccagcgacag cttaaaccaa tgattatagg
    32161 ttgctcgccc atgcctcagc aaggttaggc caaaccgggg tcttttaagc cttgcccagc
    32221 aggcaagcgg tgaggcagta aactattgtc atacagggta gtgccccatt tgaacggcga
    32281 ttacagattc ttcggcgctc acggatggac actctacaat gtaaccaccc ctttgcttga
    32341 ttttccgaca tttccgagtc agaatttgct gtatcgttca cggcatcttt gacatgtcgg
    32401 cacggaggct tggaccaaac gcagttttct ccagggacaa ccaacaggag gacttcccaa
    32461 atggtttcgg cagcaaagag ctcagagcaa gggtcgtaca ctttcagtcg ccgcaacatg
    32521 cttttggctt caggggccgt gggattgggc ttggtggccg ggaaaagccc tgcattctct
    32581 gcccttctag cggaaggggc tcaagggaca gcgcagtggc ccgtcacgtg ccgcgatgtc
    32641 atgctcaaga ggacaggcga gcccgattgc tgggctgcca tggccgcata caacgtggat
    32701 ggtgtcgagt cggacattac ggacgatctt cgcttgccgt cgttatacca aggggaccgc
    32761 gttttcagcg ttgccgagcc acagtcgaga caggaactca aggcagccct cgaagcggct
    32821 aagaaacgaa tcaccgcctt ctgtatgcat aatcgttttg aagaacggcc tgagtttgaa
    32881 ctcgaatggt gcaaaaaagt cgctgtagcg gcaaaggaat tgggggtccc ggccattcgc
    32941 attgatgttg ttccccgtcg gatgccgcga acagagttcc ttccctttgc cgtgaacctg
    33001 cttcgaaaaa ttgtggatgc cacggaggac accggcgtga agtttgccgt cgaaaatcac
    33061 ggcaatacca ccaatgatcc aacgtttttg gatgccctgt ttgaaggcgt gggttcacca
    33121 cggttgggct taacgttgga tacgggcaat ttctattggt ttggccatcc cctcagtcgc
    33181 ctttacgaga tatacgaacg gtttgcccat cgtgcatacc acacccattg taagagcatt
    33241 caatatccag ccgacatgag agacaaggaa cgtcctatgg gctgggaata ccaaaagtat
    33301 cactgcccca tcgaccaagg cgacatcgac ttcgccaaag tcattggaat cctgaggaag
    33361 gccggttaca gcaacgactt gtgtatcgag aacgaagggc tgggacgact cagcgcggaa
    33421 gaagccgttc agaccgttcg caaagaggct cagctcttac ggaatcttca gtctgccatc
    33481 actcaagaag ggcgttttat gccggtggcc cgggcgtgag aagttgctcg ccgcccacaa
    33541 cctgtttttc ctggccttcc catctagcgc gaattcgcgt ttacgccgga tgcgtaaact
    33601 agtgatgtgc gctttttctg gtggaatggc tgtttcctgt agaatcggca aaataacaaa
    33661 agttttccgg tccgtctgtc gatacaggaa acgccatgtg gtgtaaccgt taccagcaag
    33721 acataccggc gttcagagga cctgacggac agtgggtttg cctccgttgc ggctcaggaa
    33781 gtcgctccac ggaaaccggg gctacggagg cggaacattt gatcgcccag gttggttctt
    33841 cttcgagttt cgaaacgccc tccgtctcgc cgaatgagaa aacggaatcg gacacaccac
    33901 gttatgatgc ctgggaattg gatgaggaac ttgcccatct caaaaggctt ctcagcatcc
    33961 atcaggttaa accaggtgaa ttggtcaccc gatttgatgc ctatcatgct ttggcaaagc
    34021 cagcccactt acggaaatcg caagtccatc acgatttatt gcgcgctgag cgactcgctg
    34081 aagccgaaca cgggcccgcg tggtggctct ttctttgtag cgtcacgatc ttcggctgtg
    34141 gtgctattct cctcgggtgg gggctggctt taaaccacga acgcctattg atttggggaa
    34201 tcccaatctt tgtcgcagga ctgctcgtcc tcattgcctc gatcttgttg cccattttgg
    34261 agcggcggtc atcgcgtcta tcacctcatc ttcccatgaa acgcgtgaaa accaacatgg
    34321 agacacttca ggcggggcga gacgtctgat ctcagtttcc cacgatggaa gacggcaaac
    34381 ccgcgttatg gttcgtcgtt agtgaaaagg gccgcccaat cggcagatac cggagacgag
    34441 cgggcaatct caaaaaagtt agccgcatcc tctgcgcgac cactgcgaaa tagcataacc
    34501 ccaagcaaaa aagccagcat tgggtcgtgg tttttctttt cacactccgc tgccaagtta
    34561 gcaaggtgtt ctcggaagtc gagttcccgt tcgtgataca gacgctgaac cgaaaatggc
    34621 ttcttcggcc aggtggggtc aattctcaaa ccacggcgga atgcctgaaa ggccagctca
    34681 tattgtttcg tagcgattaa agcaaatccc tgccggaaaa gtgattccgc cacatcgggg
    34741 gcggcctttt ccgcctcctt gtatttgaaa taggcatccc gatattgccc ggccgaaaac
    34801 tgctgatctc caaacgtcag gaaccgactg gccagtgccc gttgttgttc gttgcttggt
    34861 cgaaacttga tcggctccgc gacggcaagc cccgacggac cacggttttc gttaacttga
    34921 cccgcttgcg cagccgcgga ggaaccctgg gcgacgcccg aagatgacgg ttggggccgt
    34981 gtagttccga tataacggtt cgtcacggcc gcgagatccc ggaggagcgg cgcggcggga
    35041 tcgaagtaga tcaagcgcgg ctggtagtaa acagggacgg tccgaggata gcaataggct
    35101 acggggaccc gatagtaaca gtaaccgggg ggaacaccag gatacccagt gtcccaaaca
    35161 acgtcctgat acgagataac gccgccgccc acgcccgaaa cgataacagt ggtttgagaa
    35221 atatggaccg aaggcccaca cggccggagc ttgtgtcgat cgaagtaacc tccagcactt
    35281 ccagacgaca cgcggtcgtg aggcgggtgt acgtaacgcg agtgctcgtt ccctgtgaac
    35341 ggtcgtccat cgcgtgatgg gcgagacgtc ggctccatgg agaacgcgac ccagccgata
    35401 acgacaaggc agaccgccgc acaccaacac actgggtgaa gcgatctcga ttgattcgcc
    35461 gacattgccc tcacctcctc aacctgccag agcgaattag cagcatccca gccctgagag
    35521 accttgccca cgcccacctc tagaattcta ccaagtccgc cttgacagac caacagcata
    35581 attgccgcct ttcagaggac gaggaaagat ttgtgtcggc catagccctc tgaaaagggg
    35641 aacacttttt gatccttgtt gaagagcgca atcgattcca atcgaaaata gcgtgcctac
    35701 ctcgcccaca agctctcgaa cgaagtgaac gaacgaccaa aggcaaacgc ttgacgtctg
    35761 ttgttgccgg ttttctaatg gaaccggccg tgagcggcat cctgtgtgcg ggcggtgtga
    35821 gaacgtcaat ccatgagaaa aaacgcccgt ggagaacgcc gttcttgctc cttgcgggga
    35881 gttgggtgga agattgtccg taaactgaca aacttgcgag acgttgtgtc atgatgatca
    35941 atgttttcgg ggagcgcgac ctggaattgg caacgttttc tacatcaatg cgttttcgtt
    36001 acttgatgcg attcgccaga acatcgaaat tggtccaagt tggatcgacg gccggtcgac
    36061 gtccgtcccg gattgtggcg cactatctgg tgacaatgct gaccgcgtgg ctaattctgc
    36121 ccgttgcgtg ctgctctgcc gaagattttg agtggcaatc ggcggacccc gctgaggtag
    36181 gtatctcccg agaggccctc gatgaacttt gccgggccct ggccgagcgg aggacaaagg
    36241 ccttttttgt ggcccgtcac gataaggtcg tttgcgaatg gtattctcca gatcatggtc
    36301 ccgatcgaaa gcatggtacg gcctcgctgg caaaggcgat tgttgggggt atggccttgg
    36361 ccgtcgcact tgatgatgga tatgtcacct tggacgatcc tgtctcgaaa tacatcccgc
    36421 aatggcgtga agattcccga aaggcaaaga ttacgattcg gcatttgggg tctcacacgt
    36481 cgggtttgga cgactcccgg ccgaacgaag aggccccctg gaaagatgcc ttctggcgac
    36541 gagaggatcc cccaaatgat cctttcacga tttctcgtga tcaagccccg ttaaagtttg
    36601 cccccggcga gggctttcaa tacagtaacc ccggcatcgc gatgctaaca tacgccttgg
    36661 ccgcggccat gagaaatgcg cccgacaaca acatccgcag tttgttacgc gaccgagtgt
    36721 atcgacccat tgggattgcc gacaacgagt ggtcaattgg ttacgggaga acattcgagg
    36781 tggatggctt gccgcttgtg gcggcctggg gtggcggaag ctttaccacc cgcgcgaccg
    36841 cccgcattgg acgcctggta ctcaaagaag gtatgtggca gggcaagcag attctcagcc
    36901 gggaggcaat ccgtcaggtg acgcaaagcg cgggactgcc gggcgactgt ggaatgggtt
    36961 ggtggactaa tgcaggcgag cggtttcctt ttttgccgcg agacgctgtc tggggagccg
    37021 gggcagggga tgaggtcctg cttgttattc ccagcctcga tttgattatg gttcgctatg
    37081 gaaatgatct aggtccccag gtaccctcgc aagagaacat tggtcggttc cttttccagc
    37141 cacttatgaa ggcggtcctt tcgcactccc aagtctctgg ccaaacgcaa ccaccgtatc
    37201 cgccgagtcc cgtaatcagg gagttgcaat gggcaccacc ggaagagatc attcggctgg
    37261 cacgagggag tgacaactgg cccatcacgt ggggcgacga cgatcaactc tacactgcct
    37321 atggagatgg ttgggggttc gaaccacggg ttcccaaaaa actcagcctc ggattggccg
    37381 ttgtccgcgg aatgccgccc actattgagg gtgcaaatat ccgctccgaa tcggtggaac
    37441 aatacggaga cgggtcgcgg ggtaaaaagg ccagcgggat gctgatggtc gatggcgtcc
    37501 tctacatgtt ggtccgaaac gcggggaatg cccagttggc attttcccgt gatcgtggcc
    37561 ggtcttggga atgggcggat tggaaattca ccgttagctt cggttgccca gttttcctga
    37621 attttggtcc taactacgcc ggtgcgcggg acgattatgt gtacatttat tcaccagaca
    37681 gcgacagcgc ttataccccg gccgatcgga tgatcttggc acgggtagga aaagatgccc
    37741 ttttaaagca agaggcttac gagtattttg tcgggcttga tcctacggga cagcctcggt
    37801 ggagtcgcga tatccatcaa cgtggtgcgg tctttgttca tgcggggcga tgtggccgat
    37861 cgggcatcac gtacaatccc gccctgaaac gatacatttg gtatcaggtc tatccgcaaa
    37921 gcgatcaccc ggagggaccg cgtttttcgg ggggctgtgg cgtttttgac gccccagaac
    37981 cgtggggacc ttggacgact gtctttcata cagatcggtg ggacgttggg ccgggtgaat
    38041 cagggtgttt ccctacaaag tggatgagta ccgatggccg tgagatgtgg ttggtcttct
    38101 cgggggaaga ctgtttctcc ctgcgtcggg ccaaactcat agtgcgatct gccgacaatt
    38161 aaaggtctat gagggggtaa cgaatcgatc gtcggggcac aagagattgg tcttgagaag
    38221 ggcttttcaa gtagctgaac aggagtcgta agaactggcc ctagagccta tcccaaaatt
    38281 ccctctcctc cgggagaggg ttagggtgag ggcaagcgac cgtcggagtc gattgagaaa
    38341 acccgcagcg attcgagcca ccctcacccc cgacccctct cccgccgaac gggcgaggcg
    38401 agattatagg ataggctcta aacgcgatac tggcgagctg tgtgtcgggc gaggcgaaac
    38461 aacagagcga tgaattccat gaagtggtgg accgtcaagg ttggtgtgtt gctaacggga
    38521 ttgtcagcca ccatatcaaa tggtgaaccc gggccgggga caatacgttc cccatatcca
    38581 ccaagtcccg tttttgctgg gatagagctt gactggtcca cccatcgccg cgaggcgcag
    38641 gggagtgacc tgtttcaact gtgctgggcg gacaacgatc accaatacgg tttctggggg
    38701 gacgggggtg ggtttggggg gacgaatcag ctcggtcggg tcagccttgg ctttgcccgg
    38761 atcgaaggtt catggaacga ttatcggggg ttcaatgtgt ggggtggaaa agatgccgaa
    38821 acccccgcca cgtttaaggg aaagagttgg ggaacagtct gcatcgatgg cgtcttttac
    38881 tcgtggattg tgcccgacga acctgacagc ggggggcctc gagatcacta tcggtatgtt
    38941 gaattggctc gctcaaccga tcatggggcg acgtggcaga aagcgccgtg gcgatggtac
    39001 cgcgaggatg aacttatcgg acccacgttc ctcgtcgatg gtaaaaacca caaggctgcg
    39061 ccggactctt acgtgtatgt ctatttcatc cgcccagaaa ggaaggatat cactcagcct
    39121 gtggtggggt tgcgggtgca gaagccagga gcgatctttc ttgcccgcgt ttcccgccag
    39181 gccatctttc aagggcgcga agcctacgaa tggtttacgg gtataaaagg caaccaacca
    39241 gtttggggac acttggacca gaagcgacct gtatttgaaa atcccaatgg tgttgggtgg
    39301 tgcctaagtg ccatttatca ttcaggtttt cagcgagtgc ttctcgctac ggagcacacc
    39361 gaatcatctc gtggctgcat tgggcttttt gacgcaccgg atccctgggg gccgtggaca
    39421 acggttgaat actggaccgc ggacaggccg tttggtgacc agcggcccgg cagttcctgg
    39481 gaatggaaga gcaatatctt tctcatctcc ttcgccccaa agtggtttag tcccgatgga
    39541 cgggagttta ctttggtttt taccggcgcc ggcaatggac gcgataatga ctcgctcaat
    39601 acggttcgag ggcgttttcg tgtgaatgcc aatcc
//
LOCUS       DAOSUT010000012        39052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_723_length_39052_cov_9.612124, whole genome shotgun sequence.
ACCESSION   DAOSUT010000012 DAOSUT010000000
VERSION     DAOSUT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 39052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39052
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_39052_cov_9.612124"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..882
                     /gene="rpsB"
                     /locus_tag="PLD05_02000"
     CDS             148..882
                     /gene="rpsB"
                     /locus_tag="PLD05_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOP76230.1"
                     /translation="MATILVKDLIEAGVHYGHRTSRWNPKMRPYIFGRKNDIHIIDIR
                     ETLRGLLRARKYLHQLGAKGSLILFVGTKRQAADIIAEQAQRCGMPYVNDRWLGGTLT
                     NFATIRSRFQRLEELESIIHSDAINTFSKKMQSALNREYRKIYRNLHGLRTMERLPEC
                     LIIVDPNKEKNAVREARKLGITTVALIDTDCDPDDVDLPIPGNDDSMRSIELIVRALA
                     DAILEGKAAAYSKEEAAPVPSQVGVS"
     gene            992..1819
                     /gene="tsf"
                     /locus_tag="PLD05_02005"
     CDS             992..1819
                     /gene="tsf"
                     /locus_tag="PLD05_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOP76231.1"
                     /translation="MPEITAAAVKALRDRTGLPMMDCKRALEEAQGNPEVAIEILRKS
                     GQKVMEKRAGRETAFGRIGMYCEPRAKTALVELLCESAPVAKAEDFVALATELAKQLA
                     LGPGADSAEALLSQPSSQPGKTLRDVFEDTVNRIREAFRIGRILRIDGACGAYLHHDG
                     SKAGVVQVEGSQLDVARDIAMHVVAMRPTALRREDLDPALVAKEREILTEATRKEGKP
                     ENIIPKIVEGRLKDFYSQHCLVDQPFVKDPKTTVGKVAQAAGLTLLRYWYWEVGKES"
     gene            1823..2602
                     /gene="pyrH"
                     /locus_tag="PLD05_02010"
     CDS             1823..2602
                     /gene="pyrH"
                     /locus_tag="PLD05_02010"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOP76232.1"
                     /translation="MSAEEQQQRASPYRRVVLKISGEGFTRNNERGINMASVVELAQE
                     TYRAAKSGVELAIVLGGGNILRGAQFSAGTTSIQPATAHYMGMLATIINGLAFQDALE
                     ALGLETRLVSALFMQDVAEPNIHRRIERHLSKGRVVILTGGTGRPFVTTDTAAAQAAL
                     ELKADIVMKATKVDGVFSDDPAKNPHAILYSRITYREFLRQNLRVMDPEAVTKCSEHQ
                     LPIVVFNFRRPGNIERAVRGERVGTLIYAGDATTGGEDVSP"
     gene            2886..3446
                     /gene="frr"
                     /locus_tag="PLD05_02015"
     CDS             2886..3446
                     /gene="frr"
                     /locus_tag="PLD05_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOP76233.1"
                     /translation="MTEEEILFTVEEKMEKAISVLKENLAGIRTGRANPGLVESIKVE
                     AYGTTVPLKQLAAVGVPEPQQIVIRPYDPTTLKDIEKAIQAADLGFNPQNDGRVIRVN
                     VPPLSTEVRKKMVARIRELCEEAKIAIRNIRREGNKMADQAEKDKILSEDDCERVKER
                     IQELTKEYEEKANELAKHREKDVMEG"
     gene            3544..>3899
                     /locus_tag="PLD05_02020"
     CDS             3544..>3899
                     /locus_tag="PLD05_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76234.1"
                     /translation="MASEQNGLSLPEPPPSPQAASLRPRLREPVWPIFLVVVLSLVVQ
                     VMGLGLIGLVAVLRYPEARESADALRIVLTQATDEPQAILIGASGVMMGFGLLAFFAA
                     SLSRHPIRETLRLAKPA"
     assembly_gap    3900..3999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4000..4338
                     /locus_tag="PLD05_02025"
     CDS             <4000..4338
                     /locus_tag="PLD05_02025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOP76235.1"
                     /translation="GFRRRWGPWLASFLAAVLFGICHLDFTQGMFAALMGWYLGLLTE
                     RTNSLWPAVLCHATNNTIATVLALGNEHTMPGRPELVFGFATLIVLLTAWSIVGPMLV
                     VRQRDETSPK"
     gene            5129..6445
                     /locus_tag="PLD05_02030"
     CDS             5129..6445
                     /locus_tag="PLD05_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76236.1"
                     /translation="MSKSRLSLDSTPSRRQFLKATTAAVSGAMVTLPLERMVHAAGQE
                     GFKIGLIGCGGRGSGAAANAMNAGKDIKLVAMADIFEEHLRSAREQLKARYPDQVQVD
                     DDHCFVGFDAYEKLINCGVDVVLIAAASHFHPVMFKAAVDAGKHIFCEKPHALDVPGL
                     KLAMAACEEAKKKNLAVVSGLCWRYDYGVREAMKRVFDGAIGEIVAIQETYITSPYVV
                     RPRQPGWSEAYYQFKNWYHFNWLSGDQTGQQLIHSLDKASWALGDKPPLRAWGLGGRQ
                     ACIDPKYGDLFDHHSVVFEYDNGVRVFGFCRDIPGCYNETSDIIFGTKGRCILPSRPR
                     IEGATEWEYKGPKVSMYDVEHKELFDSIRQGKPINNGDYMFTSTMLGILAQMVCYTGQ
                     LITWEEAMQSQLSFSLPRYGFDIDPPVKPGPDGIYPAPIPGVTKFQ"
     gene            6557..7429
                     /locus_tag="PLD05_02035"
     CDS             6557..7429
                     /locus_tag="PLD05_02035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOP76237.1"
                     /translation="MNAPKTPLVGLTFVVVLGLLVGAKTKGDESPPIPPLDAATIEHM
                     RQVHARFDGRKGTLALFGDSITVSLAFWAPLAYECRNMPPEMERAVHIVKSYMLPECW
                     RDWRGPEFGNEGRTTIRWAFENIDGWLTRLRPETAVIMFGTNDLNDLDEQEYREKLRA
                     VVEKCLNRGTIVILTTIPPRHGRAEKAVRFAQIAREVAEQLKVPLIDYHAEILKRRPE
                     DWDGADEKFQGYETYEVPTLISRDGVHPSNPKSMMGDFSEEALRTSGFGLRNYLTVLR
                     YAEVIEKVLQVPQE"
     gene            7907..8983
                     /locus_tag="PLD05_02040"
     CDS             7907..8983
                     /locus_tag="PLD05_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76238.1"
                     /translation="MLGILSMKRRGFTLVELLVVIAIIGILIALLLPAVQAAREAARR
                     SQCTNNLKQLGLALHNYHDIHKVFVALKAGTQGTDPWDDGNNTQLCGLIPLLPFLEQR
                     ALWDQIVAGGTLNNGTRVNPFGPAAWRTDYPPFRQQTPALLCPSDPAYGKQNPASEQA
                     FNNYMFSVGDHINRNAYDRPNRGPFAHQYWCSFSDIVDGSSNTIGMVERSIYTRSGAL
                     KGDGVANLSGIDQNPTLCLATKGANGMYASGYTIHSEKAVGRRWADGRPYWNGVTTVL
                     PPNGPTCLGTANSWEWGIFSPSSYHPGGVNGLLMDGSVRFFSETIDTGILSSPQPGNR
                     GGPSPYGVWGALGSMAGGESVATP"
     gene            9213..9698
                     /locus_tag="PLD05_02045"
     CDS             9213..9698
                     /locus_tag="PLD05_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76239.1"
                     /translation="MRNWVVGLLLTVAATLALSGCKKAADNRPKRVPASVTVTYRGAP
                     VEGATVTLHPTSPDGRAAFGRTNAQGQAELGTFDVNDGAIPGDYNVTVVKMEGGDQAG
                     ETPSSEIGAMPANPAGGAPPPPAAPRHLLPAKYADINTSGLRATVNASGDNKITLELT
                     D"
     gene            complement(9753..10721)
                     /locus_tag="PLD05_02050"
     CDS             complement(9753..10721)
                     /locus_tag="PLD05_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOP76240.1"
                     /translation="MSRDLKKDSRKVPIASPGAADPLDSFRAYCQLIRLPNLFTSAAD
                     ALAGFFVTHPRIAETDLSSLWVIVFSSVAFYAGGVVLNDWVDREVDRQERPHRPIPSG
                     AVSARAAWKLGVGFLLLGLILAALVAWQRQNPWPVIVAALLTTTITFYNFWAKRFFVG
                     PIVMGLCRAENLLLGASLGIGKIAAFWPVTLALGVYVVGISVFARRETERSPRRNLAA
                     GLCLMFAGLGILGVFPLTAQRVVVGQERLWLLLAVLALWMLVRGVWTLADPVPRRVQI
                     TVTQAVMGIIVLDAAAVFVTHGPLGALPVLALVLPTTWAARWIAAT"
     gene            complement(10746..11597)
                     /locus_tag="PLD05_02055"
     CDS             complement(10746..11597)
                     /locus_tag="PLD05_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP76241.1"
                     /translation="MKLAYSTNAYMRFPFEEAVSRIARLGYTGLELLADVPHAWPMNL
                     LPGDVEHIREILNRHGMAISNINAFMMRGVNDRRQPYWYPSWIEPDPNYRAIRREHTK
                     RALKLAAELGAPGIQTEPGGPLPPGMSRRHAYRIFYDELMPCVEVAEKLGVHLLIEPE
                     PGLLIERFEQFLEFTSMIDSPAVGLNFDIGHAFCVGQSPEVWISTMAPLTRHYHIEDI
                     SAQRVHHHLIPGEGAIDFAAVLKAIRDSHYSGWITVELYPYIDDPDAAGQKALRYLQS
                     VAQSIGN"
     gene            11913..12569
                     /locus_tag="PLD05_02060"
     CDS             11913..12569
                     /locus_tag="PLD05_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOP76242.1"
                     /translation="MTDYSKLYDAIVTGDHRLAVEVTKAALEANVDPSELIAKYMIPA
                     MNEVGNRFACNEYFVPELLIAARAMKASLELITPRLAETGAKRVGRVVIGTVKGDLHD
                     IGKNLVASMLEGAGFEVIDLGVDVPAEKFVQAAQEKPGTIVAMSALLTTTMLNMKSVI
                     EALQQSGLRDKVRIMVGGAPITQQFADQIGADGYGENASAAVQVARKLAAELTQVTAP
                     "
     gene            12698..14332
                     /locus_tag="PLD05_02065"
     CDS             12698..14332
                     /locus_tag="PLD05_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOP76243.1"
                     /translation="MSDHAASESQKPGFRHLLGSGAFPCGVEIVSTRGVPANPEAIPP
                     VVFARELLSLPQVGWISITDSPGGTPMLPPDWIAHFVEDRDRVVIHMTCKDSNRSGLE
                     SFAWRLAAEGFWNVLALTGDYPVTGFGGRPQGVFDLDSVSLIALLKAMNDGLEVLSRS
                     GKPERFPPTQFFIGCAVSPFKRYERELVPQYFKLIRKIATGAQWVITQLGYDMRKYHE
                     VKLFLAARGITNIPVIGNVYLLTRTIAKLFHSGKLPGCVVSDDLMALCDKYGAGPDRG
                     RKFFAELAAKQLAVLKGLGFSAGYLGGLSKPETFSEIMEILGSFAPDDWKIFLREIQF
                     ALPDEFFFFEHDPDTGISSPDRINRQYLASLQKPKRSRYVTLGYRISRWVHALLFTRD
                     RGLWNLAKRLYARWAKKPKPPLPARALYKLEQFSKFMMYGCQDCGDCSLPDCAYVCPK
                     RWCSKCGRNGPCGGSADGRCELQDKECLWAIAYERLKAFGEAESMLEGPPVVYNAELA
                     HTSSWANTYLDRDHHRPREAKPPDGNQTPEGPKANG"
     gene            14442..17186
                     /locus_tag="PLD05_02070"
     CDS             14442..17186
                     /locus_tag="PLD05_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOP76244.1"
                     /translation="MIALIQYLQASVPVSPGQPPRWDLSGYRVVLPTGWSSRRFLARL
                     ADIAAGQKLVLFPPDVITVGRLPETLYPHHRPLADEATCLLAWWAALKELAYADEKLL
                     HRLFPAYQPTMKPTQQLALAQLLASFHREVLGAGYDCQTVLKEALKHDPKFPDQQRWE
                     TLCKLEEYYLKALHGCGLWDIDFARREALKNNECRTDREILLVGLVDLPPIVRDMLRQ
                     LGNRVTALVLAPESLHDHFDDLGCLIPEQWQELPVRIPCDKIAVCHQPIDQAHAVIDA
                     LAKWGDRFSVEEISIGLPDERLLPLVQEALEQHDLVGRFGAGRPVLQSSVWVLMRSLQ
                     QFVSQPTAQAFAALIRHPQIERWLRTRFDQSRDFLTEMDEWIGERLPLLMDAQAMAKE
                     HGPVKEIWEAVWNLLQPEEWTKPQRIDTAGEMIRRVLLNCFGQPDDPDGLQQNPQTLE
                     ICRIILRAIQTWQQVPQVLLDQVPWQGTPLDLLHWVEQQLVHQRLAAEAPGPSIELMG
                     WLDLPWDDAPATIVTMVNEGYIPSAVRSDPLLPPRLREWLGIADNRQRFARDAYNLSL
                     LVSSREEYQLIAGRQNLDGDTLLPSRFLFSPDDETMVKQVRAYFGLGDQSPRRRPSRW
                     TMAAPNNQHLEPPRPQPVEPITEIRVTQFRDYLACPYRFYLRHILKVATVNDQIEELD
                     PAGFGELAHQVLCEFARSRLRDSTDADAIAEHLIRRLEAIIKQRLSAAPLPAVPIQIE
                     QLKARLRAFATFQAEHAKQWRILHAEYEPKEPVLLDVDGKTIRLKGRIDRIDQHRETQ
                     WYLILDYKVTDTDNTKPEQSHHKKVGGQRVWIDLQLPLYRRLAGHVIKDRNVSLGYIL
                     LPKKTESTGLREATWTEEDLAEAEETARDVVRRIWREEFWPPKEDAEGRFPELSPILP
                     "
     gene            complement(17813..18340)
                     /locus_tag="PLD05_02075"
     CDS             complement(17813..18340)
                     /locus_tag="PLD05_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOP76245.1"
                     /translation="MTTMTLPDQVIPLPADPRLAHALVAAVEDALTMCNARAKLVGIN
                     CVPIFEAGAITGVIGLHGKATGFLTFNTCEQVAIALTSGFLQEPVSGINGQVVDTVGE
                     LTNLIAGGLKKRVSDTPWGIQSVTVPSVIVGHNYQIVYARGITYLSATFEHENPEAFL
                     LDQRLLQAAVSLLRV"
     gene            complement(18585..19751)
                     /locus_tag="PLD05_02080"
     CDS             complement(18585..19751)
                     /locus_tag="PLD05_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP76246.1"
                     /translation="MTTRTTRRRFLQQTAVTMAGLSVATPWLSAKAAESTAPTPPPVP
                     RWRGFNLLDFFQAMWRQVEGDFVKVTPTPEEDCQLIAELGFDFVRLPSDYWLWIDSDW
                     PRTKKLKPDDMFKIREATLEKIDQSIETCRKYGLHVNFNLHRAPGYCINDPEREPVSL
                     WSDKVAQEAFVYHWEMFAKRYRGIPAKELSFNLVNEAPSPRPGYMTAEDYCRIMTQAI
                     EAIRKVSPDRLIFVDGLNVGTKVVEGLIPLGVPQSVHAYYPAQISHYRASWVDRNSSF
                     PDPTWPIRNPDGSVKIGRKQLEEHFAPWGELRKKGIGVHCGETGCFNRTPHEVFLAWM
                     EDVLDILKTHDIGWALWNFRGSFGVLDSGRQDVAYEDFHGHKLDRKLLNLLQKY"
     gene            complement(19837..>23118)
                     /locus_tag="PLD05_02085"
     CDS             complement(19837..>23118)
                     /locus_tag="PLD05_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP76247.1"
                     /translation="KGFTIEVVFRYFGQGSQLGNGRPNGMIFAQGDGYWHGLRLYCET
                     PTGQLRFEMGRPQPQSAFGLSSSVGVPKGVWQHVAITWDGEQLRLYWNGVLTAAAAYA
                     GPYTPPNGPLRIGFADAGIGSLQLQVAEWVAYSTVLPADEIAGHAIAALPGPLVATRL
                     SARPATWAETWRLATMSAAAGNWRDAEQQWATLAEQTDVPQAIRFLARFASAIAKQRR
                     GVTGPAIQTWAALSQDISTPEILRLNSAAMCVVRRRGIIWAVAAPELYEALLKSTDWS
                     AEERLSLLLLSGQAALQRGDPALAQQRFEEARSLPTTSKDIAWDLALRIAHCRRAAKD
                     WEAARQAYRQIAEATDAPMPVRSLALLAWASTYERASQWADAAKAYRDIGGSPILHPV
                     HRWEAEELAREMDRLAQGLPRRDPSWHRTPAPVVPNPGLVLHVAPQGNDANAGTTEAP
                     LGSLEGARNRIRQIKSTSGLPRGGITVLVHGGIYPVRQTLELTEEDSGTPDSPIVFSA
                     QENEVPIFTGGVVLTGFQPVRDANILRRLPEEARDKVVQVDVRANEVEDLGTLGSRGF
                     GLAGYPTHPWVDVYCNDQPGTLARWPNDGFVNIKGLIGGSGDQPGTWTVEGRPLTWDL
                     NNEIWMYGYWRYLWAGTTIRVASIDRKTGQLRTAGRSNYGFADGMPYYFFNVLEELDQ
                     PGEWYLDRKSGILYLYPPQDESSLRVTLPVLAAPFVRTKNTSHVRFVKLTFECGRSEG
                     FVVQDGTDVALLGCRLLRLGTNGVVVLGGTRHLIMGCDIGTVGAGGIRLAGGDEKTPT
                     PGGHVVENCHIWDFTRVDRNYAPAVHVDGVGNVIRHNLFHDSPHHGIRLEGFDHLVEL
                     NEVHSVVYESDDQSGIDMFGNPISRGNVLRWNFWHHIGSGHNVAGQAGIRLDDMISGV
                     AVYGNVFYRAAGGQFGGLQIHGGKDNWADNNLFIACKAAVSFSAWSEDRWLKTVRDWL
                     ENARRRGLDVSKPPLSEKYPELLQLEKDANRNFILRSLVISCGEFAIRDPGVNVFFDN
                     HAAEGDIGFVDPFHRQFGLKPDSPVFQWFDFRPIPFEEIGLYASPERATWPITHTVSP
                     KYVGK"
     assembly_gap    23119..23218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23219..23865)
                     /locus_tag="PLD05_02090"
     CDS             complement(<23219..23865)
                     /locus_tag="PLD05_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76248.1"
                     /translation="MKPTCPSCHQEIPVEDVDLDTGLGRCRFCNEIFEIPELVEHIQK
                     IQVKDTKEPKSPPVRPADTRIRLVRKEDKLLIYLPPWGLRGPTIALFLFSLFWLGFIA
                     FWTAGALGIFFQPGKAPRWENIIFSLFSIPFWLIGFGMLGGALSGMLSRRIVYLDSSW
                     LESRWECLFVKFRKQISREDVQMARVASPPVFRSTTNDQPNPTFGIEIIYQGGKI"
     gene            complement(23929..27690)
                     /locus_tag="PLD05_02095"
     CDS             complement(23929..27690)
                     /locus_tag="PLD05_02095"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOP76249.1"
                     /translation="MLGLFARGFARSCWRSLLAILVCGGLGLMASSSSAEVRGEPVRT
                     WTDSITIPTYPWKEDPNPKFWALEGGPRLSTTVRGSIVYPYLMQDHLLRVKEDRTYRA
                     VYLENEYLRVMCLPELGGRIHSVVDKTTGEEMFHLNKVIKPGMIAMRGAWISGGVEWN
                     PGPHGHTVLCLEPVNVVNGQNPDGSAYLEISSTEQIFRTRCIVRVTLHPGRSYLDEQI
                     ALENPTDGMHPYYFWNCTAFPNKTGTRFIFPMSLGTDHNAREFFRWPIHEGKDLSWLK
                     NYETYSSIFAVKCTHDFFGAYDVDADRGVVQWADHRQLSGKKAWTWGEWDFGRMAEQD
                     LTDDDGPYIEVQSGPLPTQSDYGRLAPHQRVAWQEWWYPAHGLGDGFEYATRDAVVNV
                     LRRRDGVQVRILGTGVFPRANCVIKAGKTVLRQATVDLSPLQPAVLEIPVNPSEAITI
                     EVRSANGEVLVNHRSPLEVLPVEPPDPATFRETPDDKKTAEEWYRKGEKYDLATDRLA
                     ARRHYRMALERDPGYTPARRALAILDFEAGLYPAAVDALKDVVARDPNDGWAWFYLAA
                     SHFRLKNLPDALNAAQKAVALKTPGVPAADLLGRILMVQNNHVEAEKAFRQALEQSPD
                     DSVAMDHLILCLVAQKREREALELIRRRTEGNTTAILPVWALAQIGGDKEKSVLIARA
                     NALGDPQFEVLEVALFLDEVGMSAEAEDLIRTVLIENPSQRRCDFMPYYYLAWFAGKR
                     QDQAAVKKWLAAALEHPSPRRFASRVEEVEILRFATREQPADAQAWFQLGCVLANLGR
                     IEEAVPAWQKAVELDPHNSVALRNLGLEATTRNDLTTAERFYRQAIQARPDDQTLYRD
                     LAEILIASNRRSEAIALLEAMPISGVRRADVAVMLAQAYLDEDRYDDCLRLLENTPHF
                     TNWEGQDIVWRLFNRAHILRGRQRLDQGDARSALADFEAALTYPENLGVGRSNKPIEA
                     PAQYWRGRALVALGRYREARDAWVVGAELPAVPGEQDEYREKCRQALQELPPVPEDPI
                     FIHGPTYRCFKIERDLVLTGAMDDPLWQAAPVAVLNEPVEGRPGRYRTTARLLYNDRY
                     LYVGFQCEDELVWGTHTERDAPVYEEECVEVFICPTGQPRLYYEINVSPLNTVFDAFI
                     LNGRPVGGPRINFRGLKDYTCEGLVTRVFIDGKLGEPGARGWSAEYAIPFSSLVGCVP
                     GTPKPGTTWFLNLFRIDAPDPKKPEFYSWVPTTAIDFHRPWCFGMIRFD"
     gene            complement(28137..30068)
                     /locus_tag="PLD05_02100"
     CDS             complement(28137..30068)
                     /locus_tag="PLD05_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOP76250.1"
                     /translation="MQVLHVAGLRKFYGPEPVLDGVSFDVFAGDRAGLVGPNGTGKTT
                     LLKIIAGRESADGGECLVTSGVKLGYLEQHAEFPEGRTVWEEARTAVQHLIDLQHEAE
                     TTAARLAHTQDPTERRRLAARFDFLQQELHRLNAYHLDHRIEEVLQGLGLGPSYYDRP
                     AGQLSGGEQNRLLLAKLLLAEPDLMLLDEPSNHLDIEATQWLEEFLANHRAAMIIVSH
                     DRYFLDKVTNRTLELYRGTVDSYTGNFSAYLRQKEERLLVARRTYEKQREEIAKAEEF
                     IRRHHYGQKAAQAQDRRKKLERIELVEPPREIEPPPIRFPPAERSGDVVLRVENLGKG
                     FERPLFSGLTFDIARGERWGILGPNGCGKTTLLRCLLGEVLPDVGRVVRGYNVVIGYF
                     DQYLHCLPDDLPAMDAVLPTETVADDLIEQKRRDLLAKFGITGDRALTPVKNLSGGER
                     CRVALARLTATRANLLVLDEPTNHLDLWARDALEQALKQFDGTVLFVSHDRYFIDQVA
                     DHLIVFEADRVRVIHGNYSTYTALRAAQAEAKSLDADSRSGKDHADKNRTKPSQGEPA
                     SQPTKRKRRFPYRKQEDIEADILRQESRRDEILNELALPDTHRNGQRVKELKNELEEI
                     EKSLQLLYAHWEEVMEFNR"
     gene            complement(30184..30954)
                     /locus_tag="PLD05_02105"
     CDS             complement(30184..30954)
                     /locus_tag="PLD05_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOP76251.1"
                     /translation="MGLFSGKVGLIMGVANDRSIAWSIAETLYREGAELAYTHLPGPS
                     NERRVRRLLEPYNPKVLVPCDVQSDDDILNVFATLEENYEQLDFVVHAIAFAPPAELR
                     NPYVETTRQGWHVAMDISAYSLVAVTRAASRLMENGGAILTISYFGAEKVMPGYNLMG
                     VCKAALEHSVRYLAWDVGPKRIRVNAISAGPVRTLSSAGIAGFDEMQRHAAEKSPLRR
                     NVELHELASTAVFLLSDASSGITGEIVHVDCGYNIVGL"
     gene            complement(31182..32924)
                     /locus_tag="PLD05_02110"
     CDS             complement(31182..32924)
                     /locus_tag="PLD05_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOP76252.1"
                     /translation="MGNYFLDNDDIQFLFRHIDMKELARLQEAYTDNGQADYAPLDEE
                     DAVDNYWRVLEIVGQIAADIIAPHAEQIDREGNTLNEDGTVTLHPLVRENLRRMAQAD
                     LMGFTLPRKYGGLNCPNLVYTMATEIVSRADASFMNLFGLQGIAETINAFASNDIKDE
                     VLPRFARGEVTGAMVLTEPDAGSDLQAVRLRAYQDEAGQWRLNGVKRFITNGCGEILL
                     VLARSEPDIMDGRGLSLFITERSERVRVRHLEEKLGIHGSPTCELVFDDTPARLIGER
                     QRGLITYVMALMNGARVGIAAQSLGIGEAAFRLARVYAHTRQQFGGPIERLPAVAEMV
                     TDMKIAVEAARALTYETSRICDLENNNLRILETVPDLPKDEQKERKQLSRTLKRINGM
                     LTPMSKYYASEMCVRVANDAIQVLGGSGYMKDYAAERYFRDARITTIYEGTSQLQIVA
                     AVRGVASGSFESFVADFENTTYGDQELDSLKNELIEARKRVQEAIQFAKSQPTAYLDL
                     AGRRLVDSAIICIVGHLLLKQATALERKRHVARRFIHSQLPELEAKCRQVLSGDMSPM
                     DAYEVLAGPVPSLA"
     gene            complement(33034..34065)
                     /locus_tag="PLD05_02115"
     CDS             complement(33034..34065)
                     /locus_tag="PLD05_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOP76253.1"
                     /translation="MIAPNRQGEVWVFAEQEDGSLHDVVLELCGKARQLADELGVAVG
                     AVLPGHQVRGLASKLIAHGADRVYLVEDSRLAHFQTIPYARVLCTLIEKYRPQIVLYG
                     ATPLGRDLAPRVASAMQAGMTADCTDLQISDVVDPKTKELHPKLLLQIRPAFGGNIIA
                     TIVNYDRWPQMATVREGVMPLLPPNPSRNGEVIEEKVEIDESLLALRLIERHIEPRKV
                     NLKSARVIVAGGGGVGSKEDFRLIYELAGVIGGAVGASRAAVDSGFIGKEHQIGQTGT
                     TVRPALYIAAGISGAVQHRAGMEESAKIIAINTDPNAPIFSIAHYGIVGDLKKVIPMM
                     IKALRERPK"
     gene            complement(34153..35067)
                     /locus_tag="PLD05_02120"
     CDS             complement(34153..35067)
                     /locus_tag="PLD05_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOP76254.1"
                     /translation="MPYDVVVCVKQVPDTANVTAEAMKEDGTVNRAALPAIFNPEDLN
                     ALEVALEIRDRWGGTVTVISMGPPKAAEVLRDSLYRGADRAILITDRRAAASDTLATS
                     YILAQTIRTIGRYDIVLCGRQAIDGDTAQVGPQIAEKLGLPQVTYLEQLVDIVDGQAR
                     VRRNVGRGWEIVDVHLPALFTVISTANQPRPPAARRVMRFKRARIPAEIEAEVRASLG
                     NASDEEVLDAIAKRTEELRRQGLLLEQWNLDDIHADLNRCGLPGSPTKVFRVQAIVLT
                     KKGYTEIPPTEEGIRRLIHELVVDRTLG"
     gene            complement(35617..37083)
                     /locus_tag="PLD05_02125"
     CDS             complement(35617..37083)
                     /locus_tag="PLD05_02125"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOP76255.1"
                     /translation="MLRRDFCRFALLAGGWASLHQVPRLSAANEAGDAALRRSAEEAL
                     RRAVTFFDQEVSIEGGYVWAYAEDLTLREGEGKATPQTAWVQPPGTPSVGMALLQAYH
                     LTKAPLCLQAATRTAHALVKGQLVSGGWDYRIEFAPQARSRYRYRKPPTGGEKAANSS
                     TLDDDNTQSALRFLMMYDRETGMKDEVVHEAVTYGLDALLAVQRPNGGWPQRFVAPPD
                     PKEYPDVPASYPDDWPRQWPNRPYGDCYTINDNTQADALATVLLAWRIYGDEKYLVSA
                     RRAGEFLLKAQMPDPQPAWAQQYDAHMQPCWARKFEPPAITGLESQGVMRALITLFHE
                     TGEKRFLEPIPRAIEYLRRSLLPDGRLARFYELKTNQPIYFTRDYRLTTSDADMPTHY
                     AFKVGNELDAIQRAYEEALRSGPRPRLGRLPTAPRLSASLAAQARRVIEQLDERGRWV
                     ESGRLKTAPADARVTRIITTRTFIQNLRILANYLAASS"
     gene            complement(37139..38572)
                     /locus_tag="PLD05_02130"
     CDS             complement(37139..38572)
                     /locus_tag="PLD05_02130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP76256.1"
                     /translation="MSHFSRRETLKLLGTVGMSGMMAAGSACALPSVEAPSIPLTSDV
                     RRHIFDRVWKTPLIDSHEHLPDEDDCLRGPGGTPQDEWTVILRHYLDSDFRAAGMSRE
                     QYAALFEKPGSPQEKWQILAPFWPAVKNTGYGQAVRIAMREIYGIDDLSEKTVDKVRE
                     QYQATRRPGFYRRILCDLAKIDSCQVNLGPYHESKQPLLLLQDISIVGMFAGPDIRAF
                     GRPTGIEVKTLTDWYRVIDWWFDRYAKYAVAVKSQNAYSRNIDYEKVPQERAEPLFAK
                     RLNGESLSAEEQKALEDHLFWYAVGKATEHHLPVKLHTGYYAGDNRMPLSRLRLNAGS
                     ACDLCRMSPETTFVFMHICYPYYEELIAVAKQYTNAYVDMCWSWIINPVAAKDFLKKF
                     LVTAPANKIFTFGGDYRPVEPVLGHALIARQGIALALSELVEEGWLSLDDALELIDPI
                     MHGNARRVFQVEEKKQNLRSARWFREG"
BASE COUNT         8317 a        11251 c        11145 g         8139 t
ORIGIN      
        1 tccggaagcg ggtatgctac aagtctctgc cttgagcggc ccaaggtggg agtgcgcagt
       61 ttggtccagg ccgggtaaga ctttcgacta acgccggacg ccccagatgt ggggacatcg
      121 ccggtgtgct tgtaacagga gactggtgtg gcaacgatcc ttgtaaaaga cctgattgaa
      181 gcgggagttc attacggaca tcggacaagt cgttggaacc cgaaaatgcg gccatacatc
      241 tttggccgaa aaaatgacat ccacatcatc gacattcggg agactcttcg gggattattg
      301 cgggcgagaa aatacctgca tcaactgggg gccaagggct ccctgattct ttttgtgggg
      361 accaagcggc aagccgccga catcattgcc gaacaggccc agcgctgcgg catgccgtac
      421 gtgaatgatc gctggttggg cggcacattg acgaatttcg ccacaattcg tagccggttc
      481 caacggttgg aagagctgga atccatcatt cacagcgatg ccatcaacac attctctaag
      541 aaaatgcagt cggccctgaa tcgggaatat cgcaagattt accgcaactt gcacggattg
      601 cgaacgatgg agcgcctccc cgaatgcttg atcatcgttg acccgaacaa ggaaaagaac
      661 gcggtccgtg aagcgcggaa actggggatc acaactgtcg ccctgattga cacggattgc
      721 gatcccgatg atgtcgatct tcccatcccg ggcaacgacg acagcatgcg ttcgatcgaa
      781 ctcattgtgc gggcgctggc agatgccatc ttggagggca aagccgcggc gtactctaag
      841 gaggaggccg ccccggtgcc gtcacaggtc ggcgtgagct gataggctct ttaaggcaac
      901 gtcgccgcag atgggatgga acggcgtcaa tctgccgcgc agtgttttaa tgaaattgga
      961 tcatttttca ccataggtcg gaggaaattc catgccggaa atcaccgcag cggccgtgaa
     1021 ggcccttcgt gatcgtacag gtttgccgat gatggattgc aagcgggcct tggaagaggc
     1081 ccaggggaat cccgaggttg ccatcgagat cctgcggaaa tcgggccaaa aggtcatgga
     1141 aaagcgggcg ggccgggaaa ccgctttcgg ccggatcggc atgtactgcg aaccaagggc
     1201 gaagacggct ctcgtggagt tactctgcga aagtgcacca gtggccaagg ccgaggattt
     1261 tgtggccctg gccactgaat tggccaagca acttgctttg ggaccggggg ccgatagcgc
     1321 cgaggccctt ttgagccaac catcgtcgca gccgggaaag acattgcgcg atgtcttcga
     1381 ggacacggtc aaccgaatcc gcgaggcatt ccggattggc cgtatccttc gcatcgatgg
     1441 ggcctgtgga gcctacctcc atcatgatgg aagcaaggcc ggtgttgtcc aggtggaagg
     1501 ctcgcaactc gatgtcgcac gggacatcgc catgcatgtg gtggccatgc gtcccacggc
     1561 tttgcgacgg gaagacctcg atcctgccct ggtggcgaaa gagcgcgaga ttcttaccga
     1621 ggccacccgc aaagaaggca aacctgagaa tatcatcccc aagattgtgg aaggccgatt
     1681 gaaggacttc tactcccagc attgcctcgt tgatcagccg ttcgtcaaag accccaagac
     1741 caccgtgggg aaagttgctc aagccgccgg tctgacgctc cttcggtact ggtattggga
     1801 agtcggaaag gagtcgtgat ccgtgtctgc ggaggaacaa caacagcggg catcacccta
     1861 ccgccgcgtc gtgttgaaga tctccgggga aggctttacc cgaaataacg agcgtgggat
     1921 caacatggcg tcggtggtgg aactggccca ggagacctat cgggctgcca agagcggtgt
     1981 agagttggcg atcgtcctgg gcggtggcaa tatcctccgc ggtgcgcagt tttcggccgg
     2041 aaccaccagc attcaaccgg cgaccgccca ctatatgggg atgctggcta ccatcatcaa
     2101 tgggctggct tttcaggatg ccttggaggc gctggggctt gagacgcggc ttgtctcggc
     2161 cctatttatg caggacgtcg ccgaaccgaa catccaccgc cgcatcgaac gccatttgag
     2221 taagggtcgc gtggtgatcc tcacgggggg aaccggtcgc ccatttgtca ccaccgacac
     2281 ggcagccgct caggccgcct tggaactgaa ggccgatatc gtcatgaaag caaccaaagt
     2341 ggacggcgtt ttcagtgacg atccggccaa gaatccccac gccattcttt atagtcggat
     2401 cacgtaccgg gaattcctcc ggcagaacct ccgcgtcatg gacccggagg ccgtcaccaa
     2461 gtgctccgaa caccagttgc ccatcgtggt tttcaatttt cgacgacccg ggaacattga
     2521 gcgggcagtc cgtggagaac gggtgggcac cctgatttac gcaggcgatg ccaccaccgg
     2581 aggggaagac gtcagtccgt aatccgtcgg tgtgatgccc ctacgacggc gcatggccct
     2641 ccgtgacgaa gtgatacatc cgtaggggca ggcccccgtg cctgcccgtt gctgaaattg
     2701 gtatgggtat gccccagtgc ctgcccggcc gcgcagttgg taggggcctg accctacggt
     2761 aagctcggtg gctggtgatt ccttttgcgg attgttacaa tcggcggggt attggcagca
     2821 tgtcggggtc gttggatatt ttgtgataag cgggatgttt ctttcgcgga tggaaaggcc
     2881 ttcgcatgac ggaagaagaa atccttttta ccgtcgaaga gaaaatggaa aaagcgatct
     2941 cggtcctcaa ggagaacctg gcggggattc gcaccggccg cgccaatccg ggtttggtcg
     3001 agagcatcaa ggttgaggcc tatggcacga ccgtgccgct taagcaattg gccgccgtgg
     3061 gggttcccga gccgcagcag atcgtcattc ggccttatga cccgactacg cttaaagata
     3121 tcgagaaggc tatccaggcg gccgatctcg gctttaatcc gcagaacgat ggccgcgtca
     3181 ttcgcgtcaa cgtcccgccc ctttctaccg aagtccgcaa gaagatggtt gcccgcatcc
     3241 gggaactatg cgaggaggcc aagatcgcca ttcgcaacat ccgccgggag ggcaacaaaa
     3301 tggccgatca ggcggaaaag gacaagatcc tttccgagga cgattgtgaa cgggtcaaag
     3361 agcggattca ggaattgacc aaggaatacg aggaaaaggc caacgaattg gccaagcacc
     3421 gcgagaagga cgtcatggaa ggttaatggg tcacaaaccg cttttgcggc acatgagcgg
     3481 agatctctcg ccgggcatct gatacgacaa agcattgacc gtcttctgag acggtgttcc
     3541 gtgatggcgt ccgagcaaaa cggattatca cttccagaac ccccaccgtc gccacaggcg
     3601 gcatcgctcc ggcctcgcct ccgcgaaccg gtctggccga tttttctcgt ggttgttctt
     3661 tccctggtcg tccaagtgat ggggctaggg ctgatcggtc tggtggccgt gttgcgctat
     3721 cccgaagcgc gagagtctgc cgacgcgctt cgaattgtcc tcactcaagc cacagatgaa
     3781 ccgcaggcga tcctcattgg ggcaagtggt gtgatgatgg gattcgggtt gctggccttt
     3841 ttcgccgcgt cgctttcccg gcaccctatc cgcgagactc ttcgccttgc caaacctgcn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcttccgacg ccgctggggc
     4021 ccctggttgg ccagcttttt ggcagccgtc ctgtttggaa tctgtcatct cgattttacc
     4081 caagggatgt tcgccgccct gatgggatgg tatttgggat tgctgactga gcgcaccaac
     4141 agcctttggc cggcggtcct gtgccacgca acgaataata cgatcgctac cgtactggcc
     4201 ctgggtaacg agcacaccat gcccggccga ccggaactcg tctttggttt tgcgacctta
     4261 atcgtgctgc tcactgcctg gtccatcgtc ggcccaatgt tggttgttcg ccagcgcgat
     4321 gagacgtcgc caaaatgatt ggtcatcgcg gacgatgacc ctcccgatga atcgggaggg
     4381 acggcgtccc cgccgccggc gctgcggata acagggcaac cacagggggt tgcccctacg
     4441 atttaggatc gcatcgaaac ctgtaggggc aggcccctgt gcctgcccca aagcgcaaat
     4501 ggcgccgggt ccgcgcaaaa tccaaacaac gcggccgccc cgccaaacgc aaacgaccgg
     4561 cgctgcgaac aaaccgggca accacggggg gttgccccta cgattgatgc ctcgaaacga
     4621 gttgaagttg tcgtttttct tcttccgcca tgtgaccaac atcctcttct ggatgagctt
     4681 cgttcttccg ccaaacttgc cgacgaatcg gcgtccccca gcttgggcat ttaacttcgt
     4741 tatccttgcc gatccggaag ggacctagca gaaaaggctg gataacgcgt ttctatagat
     4801 caaatgtcgt cgataatacg caaaaaagag cgtcacgcct ctcgccgttt ccatcgtttc
     4861 ttatctcatc accggcccca ttgcttctgc aaaccttccg tgggctgtgc ctcctcttta
     4921 gtgcctcgca gatgtcaaaa taggtacacc tcccagatag tgttcccatg ttctcggttt
     4981 tccggttcca cccgagatgc gggcacgccg cgtagcttgc ttttgccatg caggtgtggc
     5041 aagatgtttc tggcaaatcc gcccatgaaa aaccatggac gatgaaggtt gtgcttcgcc
     5101 ccgtctcaaa aatcggaggg aaaacactat gtccaaaagc cgtttatcgc tggattcaac
     5161 tccgtcgcgt cgtcagtttt tgaaggccac cacagccgcg gtcagtggcg ccatggtcac
     5221 actccccttg gaacggatgg ttcacgccgc gggccaggaa gggttcaaga tcggcctcat
     5281 cggttgcgga ggccggggaa gtggtgccgc ggcaaacgcc atgaacgcgg gaaaagacat
     5341 caagctggtg gcgatggccg acatctttga ggaacacctc cggagtgcac gcgaacagtt
     5401 gaaggcccgc tatcccgatc aagtccaggt tgacgatgat cactgctttg tgggcttcga
     5461 cgcttacgaa aagctcatca actgcggtgt ggatgtggtg ctcattgcgg cggcatcgca
     5521 ttttcaccct gtgatgttta aggcagccgt ggacgccgga aagcatatct tctgtgagaa
     5581 gccccacgcc ctggatgtgc cgggtctgaa gctggccatg gccgcctgtg aagaagccaa
     5641 aaagaagaac ctagccgtcg tttccggatt gtgctggcga tatgactatg gtgtccgtga
     5701 ggcgatgaag cgggtgttcg acggcgccat cggcgagatt gtcgcgatcc aggagaccta
     5761 catcacgtcg ccctatgtcg tgcggccacg ccaacctggt tggagcgagg cctactatca
     5821 gttcaaaaac tggtatcact tcaactggtt gtccggtgac caaacgggac agcaactcat
     5881 ccacagcctg gataaggcgt cttgggccct tggcgacaag ccaccgctgc gggcatgggg
     5941 actgggaggc cgacaggcct gtatcgatcc caagtatggc gatctgttcg accaccactc
     6001 cgtggtgttt gaatacgaca acggtgtgcg tgtcttcggg ttctgccgcg atatccctgg
     6061 ctgctacaac gaaacgtcgg acatcatctt cggaaccaaa gggcgttgca ttctgcccag
     6121 ccgcccccgc atcgaaggcg caacagagtg ggagtacaaa ggccctaagg tcagcatgta
     6181 tgatgtcgag cacaaagagc tgttcgactc cattcgtcag ggtaaaccca tcaacaacgg
     6241 cgattacatg ttcacgagca ccatgttggg catcttggcg cagatggtgt gctacaccgg
     6301 acaactgatt acctgggaag aggccatgca atcccaattg tcctttagcc tgccacgata
     6361 tgggttcgac atcgatccgc ccgtcaaacc gggtccggac ggcatctatc ccgcgcccat
     6421 ccctggcgtc acaaagtttc aatgaccatt gggagcacac gttcgcatca ttccccggcc
     6481 aagagatcgt gaaactacgg tgcaggcttt cggtccgaga gcaagcgtgc ccgcgggtcg
     6541 cgcggaggtt atcgacatga atgcccctaa gacgccgctt gttggcttga cttttgtggt
     6601 tgtcctcggg ctgctggttg gtgccaagac gaagggcgac gaatcgccgc cgatcccgcc
     6661 acttgatgcc gcgacgatcg aacacatgcg gcaagtccat gcccgatttg atggccgaaa
     6721 gggaacactc gcgctcttcg gggattcgat taccgtcagt ctggcatttt gggcaccgct
     6781 ggcctacgag tgccgcaaca tgccgccgga gatggaacgg gcagtgcata ttgtcaagtc
     6841 ctacatgctg cccgaatgct ggcgcgattg gcgcggccct gaatttggca acgagggcag
     6901 gacgaccatt cgctgggcgt ttgagaatat cgatggatgg ttgacacgcc tgcggcccga
     6961 aaccgccgtc atcatgttcg ggacgaatga cctcaacgat ttggatgagc aagaataccg
     7021 cgagaagctc cgtgcagttg tggaaaaatg tttaaatcgc gggacgatcg tcatcctaac
     7081 cacgattccg ccccgccacg gtcgcgcgga gaaggctgtc cgctttgccc aaattgcacg
     7141 cgaggttgcc gagcagttga aagttcccct gattgactat cacgcggaaa tcctcaagcg
     7201 gcgtcctgag gattgggacg gtgcagatga aaagttccaa ggctacgaga cgtatgaagt
     7261 acccaccctc atctcccgag acggtgttca cccaagcaat ccaaaatcga tgatgggcga
     7321 cttctccgag gaggccttgc gaacatcggg gttcggcttg cgaaactatc tcacggtcct
     7381 gcgttacgcc gaagtgattg agaaggtgct gcaagtgccg caggaatagt tcctccaagg
     7441 ctcagggttg atattgtccc tccgccccct actctcaaga aaaacagaaa tgccgggaaa
     7501 aaggtccgtc ggagcggagt gttcgaccgg atgttaaggg gaggcttccc attgcgatag
     7561 cgaccgaaca attttctttg ggaacacccg caaaattgtt gacaagcatt tgtcgccacg
     7621 gtagattata gctggctagg gtaaagcttt ccggctaact gctagaggac cactccgtgc
     7681 ttccccccag cagcaagcct ttcataacgc tgccttaacc ccactgcgca tgtcccctgg
     7741 agggtcagct tagacttcct aaagcaaaag ctctaactgc aaatcaccag aatgtgctat
     7801 gcgtggcggt gaataaaaaa ctgtagccgc gcgtcgtggc atgataacgt gttaaaacgt
     7861 gttaatgagt ggttttgttg ttatcgagaa acttaggagg tgtcctatgc taggcatttt
     7921 gtcgatgaaa agacgaggtt ttacgctcgt cgaattgctc gtggtgattg ccatcatcgg
     7981 tattttgatc gctttgctgt tgccggccgt ccaggcggca cgggaggctg ctcgacgaag
     8041 ccaatgcacg aataatttga agcaattagg tttggccctg cacaactatc atgatatcca
     8101 caaggttttt gtagcgttga aggccggcac acaaggcaca gacccgtggg atgatggcaa
     8161 caatactcaa ttgtgtggtc tgatcccgct attgccattc ctcgaacaaa gggccctgtg
     8221 ggaccaaatc gttgctgggg gcacactcaa caacggaaca cgggtgaatc cattcggccc
     8281 ggcagcctgg cgaacggatt atccgccttt tcggcaacag acacccgctc ttctttgccc
     8341 atcggaccct gcctacggaa agcagaatcc tgcgagtgag caggcgttca acaattacat
     8401 gttcagcgtg ggtgaccata taaaccgaaa tgcttatgat cggccaaacc gcgggccgtt
     8461 cgcgcatcag tattggtgca gcttttctga cattgtggac gggtcaagta atacgattgg
     8521 aatggtcgag cgaagcattt acacccgctc cggtgcgttg aagggtgacg gcgtggcgaa
     8581 tctttcgggt attgatcaaa acccgaccct ttgcttggcc accaagggtg caaacggtat
     8641 gtacgctagt ggttatacca tccacagtga gaaggccgtt ggcagacgct gggcagatgg
     8701 acgtccttac tggaatggtg tgaccaccgt gctcccgcca aatgggccga cctgtttggg
     8761 tacggctaat agctgggaat gggggatttt ctcgccgtcc agttatcacc ccggtggtgt
     8821 caatggatta ttgatggatg gatcggtaag gttcttcagc gaaaccatcg acacgggtat
     8881 tttgtcatca ccacagcctg gcaaccgtgg tggtcccagc ccgtatggcg tctggggagc
     8941 actcgggtcg atggccggcg gcgagtcggt cgccacgccg tgatccggaa catcgccacg
     9001 accagaatcg cgcgtgaaat tgtgatcaat gggtgatgta ccgccccatc ttgtgcctgg
     9061 gcgttgaccg cccttggaca gtgatgaggc ttgggtttta tggatgttct cgacgtgtac
     9121 caccgttgtc tgcggcgatg cctacagttt gacaatcgcc gcgctgacgc gggcggagtt
     9181 ggcgtattca gaactatgtg agggccttca caatgcgaaa ttgggttgtc ggtttgttgt
     9241 tgacagtcgc agcaacgctg gcattgtcgg gatgcaaaaa agcggcggat aaccgtccca
     9301 agcgggttcc ggctagcgtt accgtaacgt accgcggggc accagtggaa ggcgcaacgg
     9361 tgacgctcca tccgacttct ccagacggta gggccgcctt tgggcgaaca aatgcgcagg
     9421 gccaggccga gctgggaacc ttcgacgtga atgatggtgc tattccgggc gattataacg
     9481 tgacggtcgt caagatggag ggcggtgacc aagctggcga aaccccgtcg agcgaaatcg
     9541 gcgctatgcc tgcaaacccg gcaggcggag cgccgccgcc accagccgct ccccgtcacc
     9601 ttctgcccgc caagtatgcc gacatcaaca cgtccggcct tcgggcaaca gtaaacgctt
     9661 cgggagataa taaaatcacc ctagaactta ccgattgatt gtcgccaatg tcgcgggcca
     9721 attggcaagt cctccggtct taccccgacc gatcacgttg cggcaatcca acgcgctgcc
     9781 caggttgtgg gcaaaaccaa ggccaaaacg ggaagtgctc ctagtgggcc atgcgtgacg
     9841 aacacggccg ctgcgtcgag gacaataatc cccatgacgg cctgcgtcac cgtgatctgc
     9901 acccggcgcg gtacggggtc ggccagcgtc cacaccccgc gaaccaacat ccacagggcc
     9961 aggactgcca aaagaagcca taaccgctcc tggcccacga cgacccgttg ggcggtcaga
    10021 ggaaaaaccc ccaggatgcc caggcctgcg aacatcagac aaagtcccgc ggccaagtta
    10081 cgccggggtg accgttccgt ttcccgccgg gcaaaaacgc taatccctac cacgtacacg
    10141 cctagtgcca aggtcaccgg ccagaaagcg gcgatttttc cgatacccag cgacgccccc
    10201 agtaagaggt tctccgcccg acacaggccc atcacgattg gcccaacgaa gaatcgtttg
    10261 gcccagaagt tgtaaaacgt gattgtcgtg gtcaaaagcg cggccacaat gacgggccaa
    10321 gggttctgcc gctgccaagc cacaagcgcc gccaaaatca gccccaatag gagaaatcca
    10381 actcccaact tccaggccgc cctggccgag acggcacctg aggggatggg ccgatgcggc
    10441 cgttcctggc gatcgacttc ccgatctacc caatcattaa ggactacgcc acctgcgtaa
    10501 aacgccacac tgctgaagac aatgacccat agcgaggata ggtccgtctc ggcgatccgc
    10561 ggatgggtca caaaaaatcc ggccagggcg tcggctgctg acgtgaacag gttgggcagg
    10621 cgaatcaact ggcaatatgc ccggaacgag tccaacggat ctgccgctcc gggggaagct
    10681 atcgggacct ttcgagagtc cttcttcaag tctctggaca tgcgatcatc attttgcaaa
    10741 aaggatcaat ttccgatcga ttgtgccaca ctctgaaggt accgcagggc cttttggccg
    10801 gctgcgtcag gatcatcgat ataggggtaa agctccaccg taatccaacc ggaataatgg
    10861 gaatctcgaa tggccttcag cacggctgcg aaatcaattg ccccttctcc agggataagg
    10921 tgatgatgga cccgctgggc cgagatgtct tcgatatgat agtgccgcgt caacggggcc
    10981 atggtggaaa tccacacctc gggcgattgg cccacacaaa aggcgtgacc aatatcaaag
    11041 ttaaggccca ccgccggtga gtcaatcatg ctggtaaact cgaggaattg ctcgaatcgc
    11101 tcgatcaaaa gccctggctc cggctcaata aggagatgaa cgcctagttt ttcggcgact
    11161 tcaacacagg gcatcaattc atcgtagaaa atacggtagg catgacggcg ggacatgccg
    11221 ggcgggagtg gcccacctgg ctcggtctga atccccggtg cacccaactc cgccgccagc
    11281 ttgagtgccc gtttggtgtg ttcccgccga atcgcccgat aattcggatc aggttcaatc
    11341 caagagggat accagtacgg ctgccgccga tcgttgaccc cacgcatcat gaaggcgttg
    11401 atattggaaa ttgccatccc gtggcggtta agtatctctc gaatgtgttc cacgtcaccg
    11461 ggaagcagat tcatcggcca ggcgtggggg acgtcggcca acagttccag acctgtgtag
    11521 ccgagcctgg cgatccgcga gacggcttcc tcaaagggga accgcatgta ggcatttgta
    11581 ctgtaagcaa gtttcatcac gattgacgtc ctggggataa cgaaagttgt tgttgcggtg
    11641 aagactctag gaaaccctca tagtacagcg ctggcttgta tcgggggtag gggcgaggca
    11701 tcccctgcct ccaaccgtgt tacacaagac cactgccgcg atagggttgt gcgccgattt
    11761 gggggagtcc gccagggggc tttagctctt ggcaacggat gccgcaccct ggtaatatag
    11821 gagggcgcgc gggatgaggg gaaaaatccc gttgtggccg ttaacgatgg gttttgccat
    11881 tcctttttaa gcaagacatc tcggaggggt tcatgactga ttactccaaa ttgtatgacg
    11941 ccattgtgac gggtgaccac cgtttggccg tcgaggttac caaggccgcc ttggaggcca
    12001 atgttgaccc gtccgaactg atcgccaagt acatgatccc ggcgatgaat gaagtgggga
    12061 accgcttcgc ctgcaacgag tatttcgtgc cggagcttct cattgcggcg cgggcgatga
    12121 aggcctccct ggagttgatc accccacgct tggccgagac gggagccaaa cgtgtcggac
    12181 gcgttgtcat cggcacggtc aagggcgatc tccacgacat tggcaagaac cttgtggcct
    12241 ccatgttgga aggcgctggc ttcgaggtga ttgaccttgg tgttgatgtc cccgccgaga
    12301 aattcgtcca agcagcgcag gaaaagcccg gcacgatcgt ggccatgtcg gcccttctca
    12361 ccacaacgat gttgaacatg aagtcggtca tcgaggccct ccaacaaagc ggcttgcgcg
    12421 acaaggtgcg gatcatggtc ggcggtgccc ccatcacgca gcagtttgcc gatcagattg
    12481 gcgccgacgg ctatggcgaa aacgccagcg cggccgtcca agtcgcccgc aagctcgccg
    12541 ccgagctgac gcaagtaacg gccccgtgag ggacctttta catctgaccg gttccgatgt
    12601 ctcatcgcat ttcccgggcg ctatctcgtg gaactggatg cgatgaggga tggcctgtgc
    12661 caatgcccgt ttcctcctta gtggggagtt atcttctatg tccgatcacg cagcgagcga
    12721 aagccagaag ccgggatttc ggcatctcct gggaagtggg gcatttccct gtggtgtgga
    12781 aatcgtctcc acccgaggtg tccccgcgaa tcccgaggcc atcccgccgg tggtcttcgc
    12841 ccgcgaatta ctctccctgc cgcaagtggg ttggatttcc atcacggaca gccccggggg
    12901 cacgcccatg ctccctcccg attggattgc ccactttgtg gaggatcggg accgcgtcgt
    12961 cattcacatg acgtgtaaag actccaaccg gagtggcctg gaatccttcg cctggcgcct
    13021 ggccgcagag ggcttttgga acgtcttggc ccttacgggt gattatcctg tgacgggttt
    13081 cggcggacga ccgcagggtg tgttcgatct tgactccgtc agcttgattg ccctcctcaa
    13141 ggccatgaat gacggcctgg aggtcctttc ccgcagcgga aaaccagagc gttttccccc
    13201 aacgcagttt ttcattggat gtgctgtttc gcccttcaaa cgttacgaac gagaacttgt
    13261 cccccagtac ttcaaattga ttcgcaagat cgccacgggg gcccaatggg tcatcaccca
    13321 actgggctac gacatgcgga aataccacga ggtcaagctc tttctggcag cccgcggcat
    13381 caccaacatt ccggtcatcg gcaatgttta tctgctcacg cggacgattg cgaagctctt
    13441 tcactcgggg aaattgcccg gctgcgtcgt cagcgatgac ctgatggcct tgtgcgacaa
    13501 atacggcgcc ggccccgatc ggggacgaaa gttttttgcg gaattggcag ccaaacagtt
    13561 ggcggtcctc aagggactgg ggttttctgc gggctattta ggcgggttga gtaaaccaga
    13621 aacctttagc gaaatcatgg aaatcttggg ctcctttgcc ccggatgact ggaagatttt
    13681 cctgcgggaa atccagtttg ccttgcccga cgagttcttt ttctttgagc atgaccccga
    13741 cacgggcatc tcttcgcccg accgcatcaa tcggcagtat ctggcgtccc tgcaaaagcc
    13801 gaagcgttct cgctatgtga ccctcggtta tcgcatctca cgatgggttc atgcccttct
    13861 tttcacgcgg gatcggggcc tgtggaatct cgctaagcgt ctctatgccc gctgggccaa
    13921 aaagccgaaa ccccctttgc ccgcgcgggc actctacaag ttggagcaat tctccaaatt
    13981 catgatgtac ggatgccagg attgcgggga ctgcagtctg cccgactgcg cttatgtctg
    14041 ccccaagcgg tggtgctcta aatgtgggcg gaacggaccc tgtggtggct cggcagacgg
    14101 tcggtgcgaa ttgcaggaca aggaatgcct ttgggcgatt gcctatgagc gtctgaaggc
    14161 ctttggtgag gccgaatcga tgctggaagg accgcccgtc gtgtacaatg cagaattggc
    14221 ccacacctcg tcctgggcca atacgtacct agaccgtgat catcaccgtc cgcgcgaggc
    14281 gaaacctccg gacggaaatc agacgccaga ggggcctaaa gcgaatggct agaccgcgtt
    14341 catcgaaggg atcggatgtc gaagaacaag gtcaatctcc cttaagaacc tgcttttccg
    14401 ggcgtgtaaa gcgggttttt ttggattggt ctcgccccgc cgtgatagcc ctgatccagt
    14461 atctccaggc cagtgtgcct gtgtcgccgg gtcaacctcc acggtgggac ctctccggct
    14521 accgcgtcgt cctgcccacc ggttggtcgt cgcgtcgctt cctcgcccgt ttggcagata
    14581 tcgccgccgg tcaaaaactt gtgcttttcc cgcccgatgt gatcaccgtg gggcggcttc
    14641 ccgagacgtt atatccccat catcgacctc tggccgacga ggccacctgt ttgctggcgt
    14701 ggtgggcggc ccttaaggag ttggcctacg ctgacgaaaa gctcctccat cgcctctttc
    14761 cggcctatca gcctaccatg aaacccaccc aacagctcgc cctggcccaa ctgttggcca
    14821 gctttcaccg ggaagtgctt ggggccggtt atgattgtca gacagttctg aaggaggccc
    14881 tcaagcatga tcctaagttt cccgaccaac aacgttggga aaccctttgc aagctggagg
    14941 aatattatct caaggcactt cacggctgtg ggctttggga tattgatttc gcgcgtcggg
    15001 aggccctcaa aaacaatgaa tgccgcacgg acagagagat tcttctcgtc gggctggtcg
    15061 atttgccgcc catcgtccgc gacatgctgc ggcaattggg aaatcgcgtc acggcgttgg
    15121 tcctggcccc ggagtcactg cacgatcatt ttgacgatct cggctgcttg atccccgaac
    15181 agtggcaaga actgccggtc cgaatcccct gcgacaagat cgccgtatgc catcagccca
    15241 ttgaccaggc ccatgccgtg atcgacgccc tggcgaaatg gggcgatcgc ttctcggtcg
    15301 aggagatcag cattggtttg cccgatgaac ggttgcttcc tctcgtccaa gaggctctgg
    15361 agcaacacga tctggtcggt cggtttggtg ccggtcgtcc tgtgctgcaa agctccgtat
    15421 gggtcttgat gcgaagcctt cagcagtttg tcagccaacc gacggcccag gcctttgccg
    15481 ccctgatccg tcatccccag atcgagcggt ggttacggac gcgtttcgat cagtcacgag
    15541 acttcctcac cgaaatggac gagtggatcg gcgaacggct tcccctattg atggatgccc
    15601 aggccatggc caaggaacat ggcccggtca aggaaatctg ggaggccgtc tggaatctct
    15661 tgcaaccaga ggaatggaca aagccccaac gaatcgacac agccggagaa atgatccgcc
    15721 gcgtgttgct taattgcttt ggacagcccg acgatcccga cgggttgcag cagaatcccc
    15781 agacccttga aatctgccgg atcattttga gagcaattca aacatggcag caggttccgc
    15841 aagtgcttct tgatcaggtg ccgtggcaag ggacaccgct tgacctactg cactgggtcg
    15901 aacagcagtt agttcatcag cggttggctg cggaagcgcc ggggccttcc atcgagttga
    15961 tgggttggtt ggacctgccc tgggacgatg ccccggccac gatcgtgacg atggtcaacg
    16021 aagggtacat tccgtcggcg gtgaggagtg atcccctctt gccgccacgt ctgcgggaat
    16081 ggctggggat cgccgacaat cgccagcgat ttgcccgcga tgcttacaac ctaagcttac
    16141 tcgtctcgtc ccgggaggaa tatcaattga tcgccggccg gcaaaacctg gatggcgata
    16201 ccctcttgcc cagccggttc ctgttttccc cagatgatga aacgatggtg aaacaagttc
    16261 gggcgtactt tggtttgggc gatcaatcgc cgcgacgccg tccatcccgc tggacaatgg
    16321 ccgccccgaa caaccagcac ttggagccgc cgcgccctca acctgtggag ccgatcaccg
    16381 agatccgcgt gacacagttc cgcgattacc tagcctgccc gtatcggttt tacttacggc
    16441 atattctcaa ggttgcgacc gtaaacgacc agattgagga gctagaccct gccgggtttg
    16501 gcgaattggc acatcaggtt ctttgtgagt ttgcccggtc gcgtctccgc gacagcactg
    16561 atgctgatgc cattgcggaa catctcattc gtcgccttga agccatcatt aagcagcgcc
    16621 tttctgccgc accgttgccg gcggttccca ttcaaatcga gcaacttaag gcacgcctgc
    16681 gggcatttgc cacattccag gcggagcacg ccaaacagtg gaggattctc catgcggaat
    16741 acgagccgaa ggaacccgtc ctcctggacg tggacggcaa aaccattcgg cttaaagggc
    16801 gaattgaccg catcgaccaa caccgcgaaa cacagtggta tttgatcttg gattacaaag
    16861 tgaccgacac cgacaatacc aaaccggaac agtcacatca taagaaggtc ggcggccagc
    16921 gggtgtggat cgaccttcag ttgcccctct atcggcgatt ggccggccac gtcatcaagg
    16981 atcgcaatgt gtcactggga tacatcttgc ttcccaagaa gacggaaagt actggattac
    17041 gtgaggccac ttggacggag gaagaccttg ctgaggcgga agagactgcc cgggatgttg
    17101 tgcgaagaat ttggcgagaa gaattctggc caccgaagga ggatgccgaa ggccgatttc
    17161 ccgaacttag cccaattttg ccttaagccg cacatgccgt tgacgagtcg agcgaggcca
    17221 aacggtggtc cggcaatact tcaagaaaaa aattggcggc ctcttggggt gtcgcaaaat
    17281 accacggctg tatagaacat atcgccgcta aatggtggtt tattttgcaa catgtgttca
    17341 attttgctat agcaaaaaaa tacaggcgag cttggatttg ttcgggcggc aaatccagct
    17401 cgcctgcgta ggggtggaag ccggtacttt gggtccgtcg ggtgggttcc tcgtctgggt
    17461 ggaggagagc atcgcgtgtt ggggggtccc gacggtccct atccgtaaaa acggcctctt
    17521 ctaagtgccg gccccaagtc tcgggacggg tctcgctgcc tcttctctgt ccggttgcat
    17581 cgtcggtggc caaacattgc caccgcctgg tcgtcgcccg tctctcggac gtctttatat
    17641 ctagcaatcg ccatgccaat cggcgaggta ataagctttg gcagacataa caggggtaat
    17701 tccccgtgtt cgcccttttg acaattccac ggatggtcag gcgcaggggc ctgcccctac
    17761 ggtaagtctg gaggaacggc ggccgtcacg ttgcgggctt tggcctttcg cctcacacgc
    17821 ggaggagcga aacggccgcc tggagtaacc gttggtccaa aagaaatgcc tcgggatttt
    17881 cgtgctcaaa cgtcgccgaa agataggtaa tccctcgggc atacacgatc tgataattgt
    17941 gcccaacgat cacggagggt actgtcacgg actgaatccc ccagggcgtg tcagacaccc
    18001 gcttcttcag cccgccggcg atgaggtttg tcaattctcc caccgtatcg acaacctggc
    18061 cattgatgcc gctcacaggc tcctggagaa accccgaggt cagggctatg gccacctgtt
    18121 cgcacgtgtt aaacgtgagg aaacccgttg ccttaccatg gaggccaatc acgcccgtga
    18181 tcgcaccggc ctcaaagatg ggcacacaat tgatgcccac cagtttcgcg cgggcattgc
    18241 acatggtgag ggcatcttcc accgcggcaa cgagggcatg ggccaatcgc ggatcggccg
    18301 gtagcgggat tacctgatcg ggcagtgtca tcgttgtcat cgggcgttct ctccatagca
    18361 ggcagacggt tattctctcc gaagcatacc tctcgagtgg atcagacctt tagaaaacca
    18421 tgggatatga aacggccttg atcatccgtg tggggggtaa gacaaaaaag aaaatgcggg
    18481 gctgatatcc ccgcattcgg cgcaatcgtt aatgacgcca caacttgcat aggcaattgt
    18541 tgaccgcagg caacaccgat gttgcccgcg gaagacctat cacctcaata cttctggaga
    18601 aggttgagga gtttgcggtc caacttgtgg ccgtggaagt cttcgtacgc gacatcctgg
    18661 cgtccggagt ccagaactcc aaacgagccg cggaagttcc acaaggccca gccaatatcg
    18721 tgcgtcttaa ggatgtcgag gacatcttcc atccaggcaa ggaagacctc gtggggcgtc
    18781 cgattgaagc acccggtttc cccgcagtga acgccaatcc ccttctttcg caactcaccc
    18841 caaggggcga agtgctcctc caattgtttg cggccaatct tcaccgagcc atcagggttg
    18901 cgaatgggcc acgtaggatc gggaaagctg gagttgcgat ccacccacga tgcccgatag
    18961 tgactgatct gcgccggata ataggcatgg acgctttgcg gcacaccaag cgggatgagg
    19021 ccctccacca ccttggtccc cacgtttaaa ccatcgacga agatgaggcg atcgggagaa
    19081 accttacgga tggcctcgat ggcctgcgtc atgatgcggc aatagtcttc cgctgtcata
    19141 tagccgggcc gaggactggg ggcctcgttc actaggttga aactgagttc cttggcgggg
    19201 atgccacggt atcgcttggc aaacatttcc caatggtaaa caaacgcctc ctgagcaacc
    19261 ttgtcgctcc agagggagac gggttctcgt tccgggtcgt tgatgcagta gcccggcgca
    19321 cggtgaagat taaagttgac gtgcaacccg tactttcgac acgtttcgat ggactggtcg
    19381 atcttctcca acgtggcctc gcggattttg aacatgtcat cgggtttcag ttttttcgtt
    19441 ctcggccaat cggagtcgat ccatagccag tagtcggaag gcaagcgaac gaagtcaaac
    19501 cccaattcag cgatgagttg gcagtcttcc tcaggagtcg gagtcacctt cacgaaatcg
    19561 ccttccacct gacgccacat ggcttggaaa aaatcgagca gattaaagcc tcgccagcgc
    19621 ggaaccggtg gcggggtagg tgcggtcgat tcggcggctt ttgctgacaa ccagggcgtc
    19681 gccacgctaa gcccggccat ggtcactgcc gtctgttgca aaaatcgacg tcgcgttgtt
    19741 cgagttgtca tgagcctcgc tccttattcc aagatgcaaa cacgtcgcca gcacaaagga
    19801 tatgttgtgc caacacctac gccaacgcct tccagtttac ttgcctacgt actttgggga
    19861 aaccgtgtgg gtaataggcc acgttgcccg ctccggactg gcatacaggc cgatctcctc
    19921 gaaggggatg gggcgaaaat cgaaccactg gaaaacaggg gaatccggct tcaagccaaa
    19981 ctgccgatgg aaggggtcca caaacccgat gtctccctcg gctgcgtggt tgtcgaaaaa
    20041 gacgttaacg ccggggtccc gaatcgcaaa ttcaccgcaa ctgatgacga gtgaacgaag
    20101 aatgaagttt cggttggcat ccttttccaa ctggagaagc tccggatact tctcagacag
    20161 cggcggcttg gaaacatcca gtccacgccg acgggcattc tccaaccagt cacggacggt
    20221 tttcagccag cggtcttcac tccaggcgga aaaacttaca gccgccttgc aggcgataaa
    20281 gaggttattg tcggcccagt tgtctttccc accgtgaatt tgcaggccac caaattggcc
    20341 acccgccgcg cggtagaaca cattcccata gaccgccaca ccgctgatca tgtcgtcgag
    20401 gcggataccg gcctggcccg ccacattgtg tccactgccg atatgatgcc agaaattcca
    20461 acgcagaacg ttgccccgac taatcggatt gccaaacatg tcgatacccg attgatcgtc
    20521 cgattcgtac accacgctgt ggacctcatt cagctcaacc aagtgatcaa agccttccag
    20581 gcggattcca tggtggggtg agtcgtgaaa gagattgtgg cgaatgacgt tcccaacgcc
    20641 atcgacatgg acggccgggg cgtagttgcg atcaacgcgg gtgaaatccc aaatgtggca
    20701 gttttccaca acatgaccgc ccggcgtcgg ggtcttctcg tcgcctccgg caaggcggat
    20761 gccaccggct ccgacggttc cgatatcgca acccatgatg aggtgccttg tcccgcccag
    20821 aaccacaacg ccatttgtcc ccaggcggag tagccggcat ccaaggagtg ccacgtccgt
    20881 accgtcttgg acgacaaacc cctcagatcg cccacactcg aatgtcagtt tcacgaaccg
    20941 aacatgcgag gtgttttttg tacggacgaa gggcgctgca aggacgggca acgtcacccg
    21001 cagcgacgat tcatcttgcg gaggatacag ataaaggatg ccacttttac ggtccaagta
    21061 ccactcaccc ggttgatcca attcctccag aacattgaag aagtaatagg gcatgccatc
    21121 cgcaaagccg taattcgatc gaccggcggt ccttaattgg ccggtctttc ggtcgataga
    21181 cgccacacgg attgtcgttc ccgcccaaag gtagcgccag taaccgtaca tccagatttc
    21241 attgttcagg tcccaagtca gcggccgccc ctcgacggtc cacgtccccg gttgatcacc
    21301 tgatccgccg attaagccct tgatgttaac gaagccgtca ttcggccagc gggccagcgt
    21361 ccccggctgg tcgttgcaat agacatccac ccagggatgt gtcggatacc cagccaggcc
    21421 aaaaccacgg gatcccagcg tcccaaggtc ctccacttca ttggcccgca catcgacctg
    21481 cacgacttta tcccttgcct cctcgggaag acgtctgagg atattggcat cgcgaacagg
    21541 ttgaaagccg gttagaacca cccctccggt aaagatgggc acctcgtttt cctgggcaga
    21601 aaagacgatg ggcgaatcgg gcgtgccgga gtcctcttct gtgagttcca gtgtctgtct
    21661 tacgggataa atcccgccat ggaccaaaac ggtgatccca cctcgtggca ggccgcttgt
    21721 gctcttgatc tggcggatac ggtttcgcgc tccctccaag gagccaagcg gtgcctcggt
    21781 cgttccggca ttggcatcgt tcccctgggg cgcaacatgc agcaccaatc caggattcgg
    21841 caccaccggg gcaggcgttc gatgccagct agggtcgcgc cggggaagcc cttgggctag
    21901 gcggtccatc tcgcgcgcaa gctcctcggc ctcccagcga tgaacaggat ggaggatcgg
    21961 tgaaccgcca atgtcgcgat aggccttcgc cgcatccgcc cattggctag cccgttcgta
    22021 ggtcgatgcc caggccagaa gggccaaact ccgcaccggc atgggcgcat cggtagcctc
    22081 ggcaatttgg cgataggcct ggcgggccgc ttcccaatcc ttggccgctc gccggcaatg
    22141 agcgatccgc agggctaggt cccaggctat gtctttggag gttgtgggca aagaacgggc
    22201 ctcctcaaag cgctgctggg ccagggcggg atcaccacgt tgcagtgccg cctgaccgga
    22261 gagtagcaat agtgacagtc gctcctcggc ggaccagtcg gtcgatttca acaaggcctc
    22321 gtaaagctct ggtgccgcaa ccgcccagat gatcccacgg cgacgaacga cacacatggc
    22381 ggctgaattg aggcgcagaa tctccggggt cgaaatatcc tgcgacaaag cggcccaggt
    22441 ttgaatcgcc ggtcctgtca ccccgcggcg ctgcttggca atcgcactgg caaagcgcgc
    22501 caggaatcga atcgcttgtg gcacatccgt ctgctcggcc aaagtggccc attgttgttc
    22561 ggcatcccgc cagttaccgg ccgcagccga catggtggcc aatctccagg tctccgccca
    22621 ggttgccggt cgtgctgata acctagtagc gaccaaagga ccgggcaaag ccgcaatcgc
    22681 gtggccggcg atttcgtcag cgggaagaac cgtcgaataa gccacccatt cggccacctg
    22741 caattgcagc gaaccaattc ctgcatcggc aaaaccaatc cgcagcggac cgttcggagg
    22801 cgtataaggt ccggcgtacg cagccgcggc tgtcaaaacg ccgttccaat aaagccgcag
    22861 ttgttcgccg tcccaggtga tggccacatg ctgccacaca cccttgggga cacccacaga
    22921 ggaggaaaga ccaaatgcac tctggggttg gggacgcccc atttcaaagc gaagttgccc
    22981 agtcggtgtc tcgcagtaaa gtcgcagccc gtgccaatag ccatcaccct gggcaaagat
    23041 cattccattc gggcggccgt tgcccaactg gctcccctgg ccgaagtagc gaaagacaac
    23101 ctcaatggtg aaccccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    23221 gattttgcct ccttgataga tgatctcgat accgaatgtg gggttaggtt ggtcatttgt
    23281 cgtcgatcgg aagaccggcg ggctggccac gcgagccatc tgcacatctt cacgactaat
    23341 ttgtttgcgg aatttgacaa acaggcattc ccagcggctt tccaaccacg agctatccag
    23401 gtaaacgatc cgccgcgaaa gcatgcccga gagtgctcca cccagcatgc caaaaccgat
    23461 gagccagaaa gggatcgaaa agagcgaaaa aataatgttt tcccaacgag gtgcttttcc
    23521 aggctgaaaa aagatgccca aggcaccggc cgtccaaaag gcgataaatc ccagccaaaa
    23581 taggctgaaa agaaacaggg caattgtcgg tccccgcaat ccccagggcg gcaaatagat
    23641 gagaagttta tcctcttttc ggaccaggcg aatccgcgta tcagcagggc gcaccggcgg
    23701 tgacttgggt tctttggtat cttttacctg aatcttctgg atatgttcaa caagctctgg
    23761 aatctcgaag atttcgttgc aaaaacggca gcgccccaag cctgtgtcca ggtccacgtc
    23821 ctccacgggg atctcttggt gacacgacgg acaggtgggc ttcatgcaac gtgatcctac
    23881 caaacaacag tccgcaactt cgtcatgccc cgcttccgcc tagcaatatc aatcaaaccg
    23941 gatcatacca aaacaccacg ggcggtgaaa gtcgatggcc gtcgttggta cccacgaata
    24001 aaactccggt ttcttgggat caggggcatc gatacggaag agattcaaga accacgttgt
    24061 ccccggcttg ggcgttcccg gcacacaacc caccagtgac gagaacggaa tggcatattc
    24121 cgccgaccat ccccgcgccc ctggttcgcc gagcttgcca tcgataaaga cccgcgtcac
    24181 aaggccctcg cacgtgtaat ccttcaaacc gcggaaattg atgcgtgggc cacccaccgg
    24241 ccgaccgttg agaataaagg catcgaacac ggtgttgagc gggctgacat tgatttcgta
    24301 gtaaaggcga ggttgtccgg tcgggcaaat gaagacttca acgcattctt cctcgtaaac
    24361 gggcgcatcc cgttcggtat gcgttcccca aacaagttca tcttcacatt gaaatcccac
    24421 atacagatag cgatcgttgt ataagagtcg ggcggtcgtg cggtagcgac cgggacggcc
    24481 ctcgacgggt tcgttcagca ccgccacagg ggcggcctgc cataagggat cgtccatggc
    24541 ccccgttagg acaaggtcac gctcgatctt aaaacaccga tacgtggggc cgtgaataaa
    24601 gattgggtcc tccggcacgg gcggcaattc ttgcagggcc tgccgacatt tctcgcggta
    24661 ttcatcttgc tcgccgggca ccgcgggaag ctccgcgcca acaacccagg catcgcgggc
    24721 ttcacgatac cgacctagcg ccacaagcgc acgaccgcgc caatattggg caggtgcctc
    24781 aatgggttta ttgcttcgcc caactccgag gttctcggga tacgtcaagg ccgcctcgaa
    24841 gtccgccaga gcagatcggg catcgccctg atcgagtcgt tgccgccccc gcaagatatg
    24901 ggcacggttg aaaagccgcc agacaatgtc ctgcccctcc caattggtga aatgaggtgt
    24961 gttttccaat agccgcagac agtcatcgta acgatcctca tccagatacg cctgggccaa
    25021 catcaccgcg acgtcggccc gacgaacgcc ggaaatcggc atggcctcca agagggcaat
    25081 ggcctcactg cggcgatttg aggcgattaa gatttccgcc agatcacgat agagcgtctg
    25141 gtcatccggt cgagcttgaa tcgcctgacg atagaaccgt tcggcagtag ttaggtcatt
    25201 ccgcgttgtt gcttcgagtc ccaaattccg cagtgccaca ctgttgtgcg gatcaagctc
    25261 cacggccttc tgccaggctg gcacagcctc ctcgatccgt ccaagattgg ccagcacaca
    25321 accaagctgg aaccacgcct gggcgtcggc aggctgttcc ctcgtggcaa aacgaagaat
    25381 ctcgacctct tccacccgag aggcaaaccg ccgcggcgac ggatgctcga gggccgccgc
    25441 cagccacttc ttgacggcag cttggtcctg ccgttttccg gcaaaccagg caagatagta
    25501 atacggcata aaatcacatc gccgctggga cggattttcg attagcacgg tccgaatcaa
    25561 atcctccgcc tccgcggaca tgccgacctc gtcaaggaac agggccactt ccaggacttc
    25621 gaattgcggg tcaccgagtg cgttcgcccg agcgatgagg accgatttct ccttatcacc
    25681 tcctatttgg gccaaggccc aaaccggcaa aatggccgtc gtattgccct ccgtgcggcg
    25741 gcggatcagc tcaagggctt cacgctcccg cttctgtgcg accaggcaca ggatgagatg
    25801 gtccatggcc accgagtcgt ccggggattg ctcgagggct tgacgaaagg ccttttccgc
    25861 ctcgacgtgg ttgttttgaa ccatcagaat gcgtcccaaa aggtctgctg ccggaacgcc
    25921 cggtgttttg agagcaacag ccttctgcgc ggcgtttagg gcatcgggaa ggttcttcaa
    25981 acgaaaatgg ctggcggcca gatagaacca agcccagccg tcattgggat cgcgcgccac
    26041 aacgtcctta agggcatcga ccgccgccgg ataaaggccc gcttcaaagt ccaaaatggc
    26101 cagggcacgc cgggccgggg tgtaaccagg gtctcgttcc agggccatgc gataatgtcg
    26161 ccgggcggcc aaacgatcgg tggccaggtc gtacttttca cccttacggt accactcctc
    26221 ggccgtcttt ttgtcgtccg gcgtctcgcg gaaggtggcc ggatcaggcg gttcgacggg
    26281 caagacttca agcggcgaac gatggttgac aaggacctca ccattcgccg agcgcacctc
    26341 gatggtgatc gcctctgagg ggttaactgg aatctcaaga acggccggtt gaagcgggct
    26401 gaggtcaaca gtggcctgcc tcaagacggt tttccctgcc ttgataacgc agtttgcccg
    26461 cgggaagaca cctgtcccca agatgcggac ttgaacccca tcgcgtcgcc ggagaacgtt
    26521 cacgacagcg tcacgagtgg catattcaaa tccgtcgccc aagccgtgcg ccgggtacca
    26581 ccactcctgc caggccaccc gttgatgtgg agcgagacgc ccgtaatctg actgcgtggg
    26641 cagcggcccg ctttgaacct cgatgtaggg accgtcgtca tcggtcaggt cttgttcggc
    26701 catccggcca aagtcccact ctccccatgt ccaggccttc ttaccgctta actggcgatg
    26761 gtccgcccat tggacgacac cccgatcggc atcgacatca taggccccaa agaaatcgtg
    26821 cgtgcacttg acggcaaaga tcgaggagta ggtttcgtag ttcttcagcc aagagaggtc
    26881 cttcccctcg tggatcggcc agcggaagaa ttcgcgggcg ttatgatctg ttcccaagct
    26941 catggggaag ataaatcggg tccccgtctt gttgggaaac gctgtgcaat tccaaaaata
    27001 gtaggggtgc attccgtccg taggattttc aagggcgatc tgctcgtcga ggtagcttcg
    27061 tcccggatga agtgtcaccc ggacgatgca ccgcgtccga aagatttgtt ctgtgctgga
    27121 aatctccagg taggcggacc catccgggtt ttgaccgttg accacattga cgggttccaa
    27181 acaaagtacc gtgtggccgt gcggaccggg attccactcg acgccgccgg aaatccaggc
    27241 gccgcgcatg gcgatcatgc cgggcttgat gaccttgttg agatgaaaca tctcttcgcc
    27301 cgtcgttttg tccaccaccg aatggattcg cccgcccaat tccggcaaac acatcacgcg
    27361 gaggtactca ttctccagat acacggcacg atacgtacga tcttccttga cccgcaaaag
    27421 atgatcttgc atcaggtagg gatagacaat gctcccgcgg acagttgtgg ataaccgcgg
    27481 tccgccctcc aaggcccaaa acttcggatt gggatcttcc ttccacggat aagtcgggat
    27541 ggtgatggaa tccgtccagg tgcgaaccgg ttccccgcga acctcggccg atgaggacga
    27601 cgccataaga cctaatcccc cacagaccag aatagccaaa agagaacgcc aacaacttcg
    27661 agcaaaaccc cgcgcaaaca gaccgagcat ggcctttcct cctccaacac gacggcgatc
    27721 atgttcacga tctcagaaga gaattcttcc gaccggccca ttgcggtcgg ccgacaccgc
    27781 cctgggtggt tctcgtggcg aaccccatcc ctgccgggca aaaaagagtc taacacccca
    27841 ccgacgccaa gtccatcggc accatcggga cggacatttg tccggtcatc gaggacgata
    27901 tccttcccgg cgcggaatcg ggagggacgg cgtccccgcc gtcccgtgtg ccgtgaatgg
    27961 acgtttgcgt cgtgccgtga tttggtcgcc ggcaacggcg tccccgccgt cccgtgtgca
    28021 ccggcgctcc ggacaaacgg ggcaaccaca gggggttgcc cctacgattg gtggcgtggg
    28081 ttgacggcgt ccggcgcctg tgtgggcacc gatctcatgg gcggggatcg gggtgactag
    28141 cgattgaatt ccatgacttc ttcccaatgg gcgtaaagca gttggagact cttttcgatt
    28201 tcctccagtt cgttcttcag ttctttcacg cgttgaccgt tgcggtgcgt atcgggcaag
    28261 gcaagctcgt tgaggatctc gtcgcggcgt gattcttgtc ggaggatgtc ggcctcgata
    28321 tcctcctgct ttcggtaggg gaagcggcgt tttcgtttgg tcggttgcga tgcaggctcg
    28381 ccctgggagg gcttggtgcg atttttgtca gcatgatcct ttcccgaccg ggaatcggca
    28441 tccagggact ttgcctcggc ttgggctgcc cgtaacgcgg tgtacgtcga gtaatttccg
    28501 tgaatgacgc ggacacgatc agcctcaaag acgattagat gatcggcgac ttggtcgatg
    28561 aaataacgat cgtggctcac aaaaaggacg gtgccatcga attgcttgag agcttgctcc
    28621 aaggcatcgc gcgcccaaag gtcgagatga tttgtgggtt cgtcgaggac aaggagattt
    28681 gcgcgggttg ccgtgaggcg ggccaaggcc acgcgacagc gttctccacc gctgaggttt
    28741 ttcaccgggg tcagggcgcg gtcgcctgtg ataccgaatt tcgccaagag atcgcgccgt
    28801 ttttgctcaa tgagatcgtc ggccaccgtc tccgtgggaa ggaccgcgtc catcgccggg
    28861 agatcatccg gcaggcagtg gaggtactga tcaaagtagc cgatgaccac gttatagcca
    28921 cggacaacac ggcccacatc ggggagcact tcgcccagca aacagcggag cagggtggtt
    28981 ttgccgcagc cattgggacc gaggataccc cagcgttcac cccgggcgat gtcgaatgtc
    29041 aaaccggaga agagtgggcg ttcgaagccc ttgcccaggt tttccacccg aaggaccacg
    29101 tcgccactac gttcggcggg cggaaagcgg ataggaggtg gttcgatttc ccgcggtggt
    29161 tcgaccaatt cgatgcgttc caactttttg cgtcggtctt gggcctgggc cgccttctgc
    29221 ccgtagtgat gacgccggat gaattcttcc gccttggcga tttcttcacg ttgtttttcg
    29281 taggttcgtc gggcaacgag gaggcgttct tccttctgtc gcaaataggc ggagaagttg
    29341 cccgtgtaag aatcgaccgt tccccggtag agttcgagcg ttcggttggt gaccttatcc
    29401 aggaaatagc ggtcatggct aacgatgatc atggccgcgc ggtggttggc caggaattcc
    29461 tcgagccatt gcgtggcttc gatatcgagg tggttcgagg gttcatcgag gagcatgagg
    29521 tcaggttcgg cgaggaggag tttggccagc agtaatcggt tttgctctcc accgctgagt
    29581 tgaccggccg gacgatcata gtaggacggc cccagtccta acccctgtaa gacttcttcg
    29641 atcctgtggt cgaggtgata ggcgttaagc cgatgcaatt cctgctgaag gaaatcgaag
    29701 cgggcggcta gccgacggcg ctccgtgggg tcctgggtgt gggccagtcg ggcggccgtg
    29761 gtttccgcct cgtgctgcaa atcgataagg tgttgcaccg ccgtgcgtgc ttcttcccag
    29821 acggtgcggc cctcaggaaa ctcggcgtgc tgttccaagt aacccagttt gacgccgctg
    29881 gtgactagac attcaccgcc atcggcggat tcccgcccgg caataatctt gaggagtgtc
    29941 gtcttgccgg taccgttggg acccaccaag ccggcgcggt cgccggcgaa gacgtcaaac
    30001 gagacgccat cgaggacagg ctccgggccg tagaactttc tcaaaccggc aacatgcaaa
    30061 acctgcatgg gacatcagca tctcttggac tgccgttcgt ttggcgttgt ctccatttgt
    30121 cctctgccca gcccacggca aaggcacgtt ttaaggaagg gccaggatgg gccgcgaaat
    30181 ccctcacaat cccacgatgt tgtagccaca atcgacgtgg acgatctccc ctgtgatccc
    30241 gctggaagcg tcgctcaaga ggaagacggc tgtcgaggcc aattcatgga gctccacgtt
    30301 acgtcgtagg gggctctttt ccgcggcatg gcgctgcatc tcgtcgaagc ccgcaattcc
    30361 tgcggaactg agcgtccgca cggggccagc gctgatcgca ttcacccgaa ttctcttggg
    30421 cccgacgtcc caagccaaat agcggacgct atgttccagg gcggccttgc acacgcccat
    30481 caggttatat ccgggcatga ccttctctgc cccaaaatag ctgattgtga ggattgcccc
    30541 accgttttcc atcaagcggc ttgccgcgcg ggtcacggcg accaagctgt aggccgaaat
    30601 gtccatcgcc acatgccagc cctgacgtgt ggtctcgacg taaggattac gaagctcggc
    30661 gggtggggca aaggcgatgg catgcaccac gaaatcaagt tgttcgtagt tttcttccaa
    30721 ggtcgcaaat acgttgagga tgtcgtcatc cgactgaaca tcacacggca caaggacctt
    30781 ggggttgtag ggttccagaa gacggcggac ccgccgctcg ttggatggtc ccgggagatg
    30841 ggtgtatgcc aactcggccc cttcccgata cagggtttcg gcgatggacc aagcgatgct
    30901 gcgatcgttg gccacaccca tgatcaaacc gactttgccg ctaaaaagac ccatcagtgt
    30961 gttcgatcct tatcatcgag gggacttggg cccgtgttac ccgcgtgttg gccgaagtct
    31021 cgtcacccaa gtctgaccca ggtaatcccg ttgttcaccc ttaaccagcc ctttccccgt
    31081 cataacgcac catgatagcg cccggtcgtg gtaaccctca tgaggaaaac cgccacaccg
    31141 cgggcaaaac acacatatgc cattgtttct tgcaaatcgt ttcaggcaag gctggggacc
    31201 gggccggcca ggacctcata agcatccatg ggcgacatat cgccagagag gacctggcgg
    31261 cacttcgcct ccaattccgg cagttgcgag tgaataaacc gtctagcgac atgccgtttg
    31321 cgttctaacg cagtggcttg cttgagcaga aggtgtccca cgatgcaaat gattgcgcta
    31381 tccaccaggc gacgtccagc aaggtcaaga taggctgttg gctgcgattt ggcgaactgg
    31441 atggcctcct gaactcgctt gcgggcctca atgagttcgt tctttagact gtcaagttcc
    31501 tgatcgccgt aagtcgtgtt ttcgaaatcg gcaacaaagc tctcaaacga gcccgaggcc
    31561 acaccgcgga ccgctgccac aatttggagc tggctggtcc cttcgtaaat ggtggtgatg
    31621 cgggcgtcgc ggaaataacg ttccgccgca tagtccttca tgtagccgga cccgcccaac
    31681 acctgaatcg catcgttggc cacccgcaca cacatttccg aagcgtaata cttgctcatg
    31741 ggcgtgagca ttccgttgat tcgcttcaac gtgcgggaga gttgcttgcg ttctttctgc
    31801 tcatccttgg gaaggtcggg aacggtttcc aagatccgca ggttgttgtt ttccaaatcg
    31861 cagatgcggc ttgtttcgta agtcagcgcg cgggcagcct cgacggcgat cttcatatcc
    31921 gtgaccattt cggcgaccgc ggggagacgt tcgatcggcc caccgaactg ttgccgggta
    31981 tgggcataga cccgggccag gcggaaggcg gcctcgccaa ttcccaggga ttgggcggca
    32041 atccccaccc gggcgccgtt catcagggcc atcacgtagg taatcaaccc ccgttgccgc
    32101 tcgccaatca gacgagccgg cgtatcgtca aagaccagtt cacaggtggg cgaaccatgg
    32161 attcccaatt tctcttccaa atgccgcaca cggacgcgct cgctccgctc ggtgatgaaa
    32221 aggctcagcc cgcgcccgtc catgatatcc ggctcgctcc gggcgaggac gaggaggatt
    32281 tcaccgcaac cattggtgat gaatcgcttg acaccattca accgccattg accggcctcg
    32341 tcttggtatg cccgcaaccg aacggcctgg agatcgctac ccgcgtccgg ctcggtcagg
    32401 accatagcac cggtgacttc accacgggca aagcggggga ggacctcatc cttgatgtcg
    32461 ttggaggcaa aggcattgat cgtctcggca atgccctgga ggccaaacaa attcatgaat
    32521 gaggcgtcgg cccggctcac aatctcggtg gccatcgtgt aaacaaggtt ggggcagttg
    32581 agaccgccgt attttcgcgg cagcgtaaag cccatgagat cggcctgggc catccgccgc
    32641 aggttctcgc gcaccaaagg atgaagcgtg accgtcccgt cctcattcag ggtgttgcct
    32701 tcgcggtcaa tctgttcggc gtgcggggcg atgatatcgg ccgcaatttg accgacaatc
    32761 tccagaaccc gccagtaatt atcaacggca tcctcctcat cgagaggggc atagtcggct
    32821 tggccgttgt ccgtatatgc ctcctgcagc ctcgcgagtt ccttcatgtc gatatgacgg
    32881 aacaaaaact gaatatcgtc gttgtccaaa aagtagtttc ccacggaaac ctctcccgtc
    32941 ttttcaaacg actgtcaatg tggctgtgtg tctttcctat tagctcaaca atcgatccat
    33001 catctgccta cgatgacgtg ctcggaagac aaatcactta ggccgttccc ggagggcctt
    33061 aatcatcatg gggatgacct ttttcaaatc gcccacaatt ccgtaatggg cgatcgaaaa
    33121 aatcggtgcg ttaggatcgg tattgatcgc gatgatcttg gccgactcct ccatgcctgc
    33181 gcggtgttgc acggctcctg aaatcccggc tgcaatatac aaagcgggac gcaccgtcgt
    33241 ccccgtctgc ccgatctggt gttccttgcc gataaagccg ctatcgaccg cggcacgact
    33301 ggcccccacg gccccaccga tcaccccggc caattcgtaa atcaaacgaa agtcttcctt
    33361 acttcccacg ccaccaccac cggccacgat gacgcgggca ctcttcaaat tgactttgcg
    33421 gggttcgatg tgccgttcga tcaggcggag ggccagaagg ctttcgtcaa tttcgacttt
    33481 ctcctcaatg acctcgccgt tgcgtgaggg attcggaggc aaaagcggca tcacgccttc
    33541 acggacggtg gccatttgcg gccagcggtc ataattgacg atcgtggcga tgatgtttcc
    33601 cccaaatgcc ggtcggattt gcagcaagag ttttggatgc aattccttcg tcttgggatc
    33661 gacgacgtcg ctgatctgca agtcggtaca atcggccgtc attcccgcct gcatggcaga
    33721 ggccacccgc ggcgccaaat ctcgccccaa cggtgtcgcc ccatagagaa cgatctgagg
    33781 ccgatatttt tcgatcaacg tgcacaaaac ccgggcgtag gggattgtct ggaaatgggc
    33841 gagtcgggaa tcctccacca gatagactcg gtccgcgcca tgggcaatca atttacttgc
    33901 caacccgcgg acctgatgcc caggcaagac cgcgccgacg gcaacgccca gctcgtccgc
    33961 caactgccgc gccttgccgc acagttccag gaccacgtca tgtagggaac cgtcttcctg
    34021 ttccgcaaag acccagactt caccctggcg atttggtgca atcatcttct ttagcttctt
    34081 ctatcgtctt agaaacctaa ccgcgacatt ttttcaagag aacaatgacg ctgacttgtc
    34141 cggcccgaac gattacccaa gagtgcgatc gacgactaac tcgtggataa gccggcggat
    34201 gccctcctct gtcggtggaa tctcggtata gcccttcttg gtcaagacaa tggcctggac
    34261 gcggaatacc ttggtcggag agccggggag gccacaccgg ttgaggtcgg catggatgtc
    34321 atccaggttc cattgttcga gaagtaaccc ttgccgccgc aattcctcag tgcgtttggc
    34381 gatggcatcg agcacttcct cgtcggatgc attgcccaag ctcgcccgca cttctgcctc
    34441 aatttcggcg ggaatacgag cccgtttgaa acgcatgacc ctgcgtgcgg cgggaggccg
    34501 aggctgattc gccgtggaga tcaccgtgaa gagggccggc agatgaacat cgacgatttc
    34561 ccacccgcgc cccacattcc ggcggacccg cgcctgaccg tcaacgatgt ccaccaattg
    34621 ttcaagatag gtaacctggg gcaagcctaa cttttccgca atctgaggtc ccacctgcgc
    34681 cgtgtcaccg tcgatcgcct gccggccgca caaaacaatg tcatatcggc cgattgtgcg
    34741 gatcgtctga gccaagatgt agctcgtcgc caatgtatca ctggccgccg cccggcggtc
    34801 tgtaatcaaa atggcacggt cagcccctcg atagagggaa tcgcggagga cctcagccgc
    34861 cttgggggga cccatgctaa tgaccgtcac tgttcccccc cagcgatccc gaatttcgag
    34921 ggccacttcc agagcattca aatcttccgg attaaaaata gcaggaagag cggcccgatt
    34981 gacagtcccg tcttccttca tcgcctccgc ggtgacgttg gcggtatcgg gaacctgttt
    35041 cacgcaaaca accacatcgt agggcacgtt ctcggtctcc gtgatcagtt tatgaactgg
    35101 tggatgaaac gcatcatcag ccgatcagcc ggccgcggtc ggtgggtcaa tgtattattg
    35161 tgtgtccatc taccttgtcg atttaggggt gtcaaccaac ccgatctagc gttaacccca
    35221 ctcgagatgc cgaaaaacgg cactgggtga caatctcccc tggcccgatc gtccggatgg
    35281 ggcaacgtgg ctttccggtt gtccgctaca gcgacctggc cggcgtccac ggactgtcgg
    35341 cttcgagcga aggggaaggc ctccctatgg gttggtaaaa tgcatattgt agcgaaattt
    35401 ctccctgggc ggaacaccgc ttttttggca aaacaccccc tctaccccca caggagattc
    35461 cgggaggtac ccaaaaccat cgcgcaaaaa gcccggctgt cgcggttttt cctccggccg
    35521 atttgacaag tcggagtgtg ggttctgcac ctaaccgcag agaccaggag gcacttcagc
    35581 gtcaagggac cgaggccaaa tcggtggcga gcggccttac gacgaagccg ccagatagtt
    35641 cgccagaatg cggagatttt gaatgaacgt ccgggttgtg atgattcgtg tcacgcgggc
    35701 atcggcgggt gcagtcttca gacgcccaga ttcgacccac cgacctcgtt cgtccagttg
    35761 ctcaatcacc cggcgggctt gagccgcaag ggatgccgac agtctcgggg ccgtgggcaa
    35821 ccggcctaat cggggtcgcg gtcccgatcg caacgcctct tcatacgcac gttggatcgc
    35881 atctagttcg ttgcccacct tgaatgcata atgcgtcggc atgtcggcgt cactcgttgt
    35941 taaccgataa tcgcgtgtga agtagatggg ctgattggtc tttagctcat agaagcgggc
    36001 aagcctcccg tcgggcaaca gcgatcgtcg aagatattct attgcccgcg gaatcggctc
    36061 caaaaaacgt ttctccccag tctcgtggaa aagtgtgatc aacgcccgca tcaccccctg
    36121 agactctagg ccagtgatgg ccggtggttc aaacttgcgt gcccagcagg gttgcatgtg
    36181 ggcgtcatac tgctgggccc aggccggctg agggtccggc atttgggcct tcaacaggaa
    36241 ttcccctgcg cgacgtgccg aaacgaggta cttctcatcg ccatagatcc gccaagcaag
    36301 aaggaccgtg gccagggcgt cggcctgggt attgtcgttg atcgtgtagc aatctccgta
    36361 ggggcgatta ggccattggc ggggccagtc atcgggatag gacgccggaa cgtcaggata
    36421 ctcctttggg tcgggtggcg ccacaaagcg ttgcggccag cctccattgg gacgttggac
    36481 ggccaacaag gcgtccaggc cgtacgttac ggcctcatgc accacctcat ccttcatgcc
    36541 ggtttcccga tcgtacatca tgagaaaccg caaggcggat tgggtgttgt catcatcaag
    36601 tgtcgagcta ttggcggcct tctcgccacc ggtgggtggt tttcggtagc gatagcgaga
    36661 acgggcctgc ggggcaaatt cgatccggta atcccatccc ccggagacaa gctgcccctt
    36721 caccagggca tgggcggtcc tcgttgccgc ctgaagacaa agcggggcct tcgtcagatg
    36781 gtaggcctgc aaaagggcca tgcccacact gggtgtcccc ggcggttgga cccaggccgt
    36841 ctggggcgtc gccttccctt ccccttcgcg gagagtcaaa tcctcggcat aagcccaaac
    36901 gtatccccct tcgatagaga cttcctgatc gaaaaacgtc acggcccgac gtaaggcctc
    36961 ttccgcggag cgccgcaagg ccgcgtcacc ggcttcgttt gccgccgaca accgcggaac
    37021 ttggtgaagt gatgcccacc ctcctgcgag gagcgcaaag cgacaaaaat cacggcggag
    37081 catggggatt tcctttctcg gctgaaggtc tcaatttgtt agcgtcaaaa caaggtgctc
    37141 atccttcccg aaaccaccgg gccgagcgaa ggttctgctt cttttcttct acttggaaga
    37201 cgcggcgggc atttccgtgc atgatcggat caatcaactc aagcgcatca tccaaactga
    37261 gccagccttc ttcgaccaac tcagaaagcg ccagggcgat cccctgccgg gcaatcaagg
    37321 catggccgag gacaggctcc accgggcgat agtctccgcc aaatgtgaaa atcttgttag
    37381 cgggcgccgt gaccaagaac tttttgagaa agtccttggc cgccacaggg ttgatgatcc
    37441 agctccaaca catatcaaca taggcattgg tgtattgctt tgccacggcg attaactctt
    37501 catagtaggg atagcagatg tgcataaaga cgaacgtcgt ctccggcgac atacgacaca
    37561 aatcgcaggc cgagccggcg ttcaatcgca atcgcgaaag gggcatccgg ttatcgccgg
    37621 cgtaatatcc ggtgtgcaac ttgacgggca aatggtgctc cgtggccttg cccacggcat
    37681 accagaacaa atggtcttcc agggccttct gttcctcggc gctgagggat tctccgttaa
    37741 gacgtttggc gaacaagggc tctgcccgtt cttgcggaac tttctcgtag tcgatattcc
    37801 ggctgtaggc attttgggac ttgacggcca cggcgtattt cgcgtagcgg tcaaaccacc
    37861 aatcgatgac gcggtaccaa tcggtcaagg tcttgacctc tattccggta gggcggccaa
    37921 aggcccggat atctggtcct gcgaacatgc caacaatgct gatatcctgc aacaagagaa
    37981 ggggctgttt cgactcgtga tacgggccga ggttgacttg gcaagagtcg atcttggcaa
    38041 gatcacagag gatgcggcga taaaagccag gccgtcgcgt ggcctgatac tgctctcgta
    38101 ccttgtccac cgtcttttcc gagaggtcat cgatcccgta gatttcccgc atggcgattc
    38161 gcacggcttg cccgtagccg gtgtttttca cggctggcca aaatggggcc aaaatctgcc
    38221 atttttcctg cggtgagcct ggtttttcaa agagggccgc gtattgttcg cgactcattc
    38281 cggctgcgcg gaagtccgaa tcgagatagt gccgcaaaat gacggtccat tcgtcttggg
    38341 gtgttcctcc gggaccccgc aagcagtcat cttcgtcggg aaggtgttcg tgactgtcga
    38401 tgagcggcgt tttccaaacg cgatcgaaga tgtggcggcg gacatcgctt gtcagcggaa
    38461 tggagggggc ctccaccgat ggcaaagcac aggcggaacc cgcagccatc atcccactca
    38521 tcccaacagt tccaagcagc ttgagcgtct cgcggcgact gaaatgactc atggtatgcc
    38581 tcccgtttgt gtgaggtgtg cggtcaccgg cgtgccagag tggggacgcc tttcccggcc
    38641 aattccagcg atgattgtaa cgccccgcag tcgtcaagac gagccgtttg gaagaaaacg
    38701 gatgagagag ccgcatccca ccccaatccc accgggaggg tcattgtcaa agatgaggca
    38761 atttaccggg agaatcgccg tccgcgccga ccgaatcacc tattcccccg taggggcaac
    38821 cccctgtggt tgccctgttt ggtcacagcg ccggtggttt acgtttggcc aggcccagat
    38881 tgtttggatt tcacgcggac ccgggcgctg gtcacgttct ggggcaggca caggggcctg
    38941 cccctacggg gtttcaatgg gctcctattc gcccggaact acaggttatt ttgaaaggag
    39001 gatagccatg cgacgggttt caatgggctc ctattcgccc ggaactacag gg
//
LOCUS       DAOSUT010000013        38675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_728_length_38675_cov_9.533661, whole genome shotgun sequence.
ACCESSION   DAOSUT010000013 DAOSUT010000000
VERSION     DAOSUT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 38675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38675
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_38675_cov_9.533661"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /locus_tag="PLD05_02135"
     CDS             complement(<1..2314)
                     /locus_tag="PLD05_02135"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOP76257.1"
                     /translation="MRKLVFVCTLLIAVVSYLSTARGESAGLKLDERPTQPGEWGYRP
                     EDGSTSMVDPPAFSWRPQKEVEKWQIEVARDREFQNIAYRVQDVIWNVHCPPRTLGPG
                     RYFWRYRGHGKGGPTQWSQVRSFTISANAVPYPMPTREDLLARIPKQHPRLFIRPEDL
                     PRLKQAATNEQREVWERLVQRCEEALRNPPPTEEPPKYPPDMQRKSDEWAKIWWGNRE
                     YTIRALGTSALLGFVYQVGGHRPYGELARKILMECAKWDPVGATGFRYNDEAGMPYAY
                     YFCRTYTFVYDLLSEEERQQCRQVMKIRGEEMYRTLCPRHFWQPYNSHANRAWHFLGE
                     VGVTFHQEIPEADDWTWFAMNVFFNTYPVWCDDDGGWHEGSSYWASYIQRFTWWADVM
                     RGTFGIDAYRKPYFSKAGYYAMYLMPPHGVSGGFGDLACTRESRSNVPLMSILAVQAR
                     NPYWMWYVESMGGAPEPREFWEFIRGTLPKVSSKPPVDLPTSRVFRGTGQAALNTTLL
                     DARENVQVLFKSSPFGLQSHGYDANNAFLLTAYNERLFVSSGRRDIYGSDHHVRWMWS
                     TRSVNSITVDGVDQTPHSAATRGEITAFYTSPEVDCVEGEAGSCYLDPKAGPQGDRRL
                     LDRFTRSIVFVKPDWIVVYDRLVARRPVTYQYWLHSPEKMEVIGNGLVARVGKVECPV
                     QFLVPQNLKFHQTNEYDPNPRPRVKIREWHLTAETVNPERSVEFLTVYHPYRVGSASP
                     GQVKLEMQSGGYVLTITLGDGKAVLLLPKTD"
     gene            complement(2531..3595)
                     /gene="hemE"
                     /locus_tag="PLD05_02140"
     CDS             complement(2531..3595)
                     /gene="hemE"
                     /locus_tag="PLD05_02140"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOP76258.1"
                     /translation="MAVETPAWYDSPFLKAARREPVERTPIWLMRQAGRYLPEYRALR
                     EKYSFLELCRKPELAAEITISTVARFGFDAAILFADLLPILEPMGFELRFGPGEGPVI
                     RNHLGGPKDVDRVQELGEDRLAELDFVFEAIRLAREGLPKTIPLIGFAGAPFTLAAYA
                     IEGGTTRSFERTKCFMIEDSGAWRELMERMARSIARYVKKQVTAGVQAIQIFDSWAGC
                     LSPKHYQQFVLPYTQIVFEAIPADVPAIHFLTGNPMLLPLQAKAGGTVIGVDWRIDIG
                     DAWDVVGDGYAIQGNLDPVVLCGPEELVVHEARAILQRVRGRPGHIFNLGHGVLPSTP
                     VSNVSLLVQTVHEWNPRQIS"
     gene            3835..>4084
                     /locus_tag="PLD05_02145"
     CDS             3835..>4084
                     /locus_tag="PLD05_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76259.1"
                     /translation="MTSLSFTTWLISAHALGEGAAGNGAGADFPPFSTGAASTPQPQS
                     LTTQAERSQPSRFRRKFRTGRLGAQEGSQAGSQAGSQAG"
     assembly_gap    4085..4184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4738..5145
                     /locus_tag="PLD05_02150"
     CDS             4738..5145
                     /locus_tag="PLD05_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOP76260.1"
                     /translation="MGQKRYYVVDFAQLPGVPCPCGTAYRALADVDAFPGTIHRTEIS
                     GAARAHYHKRLTETYYILEARPGSYLELDGDTIPVHPGVCVVIPPGVVHRIVGDVVIL
                     NIVIPKFDPADEYLAELPSEDCRRCGDHDGPTR"
     gene            5300..6994
                     /locus_tag="PLD05_02155"
     CDS             5300..6994
                     /locus_tag="PLD05_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76261.1"
                     /translation="MIRHLDMQKDHGNQRTRGFIFSFAFVGIVWFLQAASVIAADWPQ
                     LGGSPSRNAVAITAALPVEWNLGAIDRATWEWKGGPQAQNILWVAPLGTQSYGSPVVA
                     GKYVLCGTNNGRGYLERFPKECDLGCLLCFSRTDGRFVWQYGAEVLPDSNQNWPEQGL
                     CSTPLVEGNRVWVVSNRGCVVCLDLEGFHDGENDGPFAQESSTGPNDADVVWEFDMIN
                     RLGVSPRYMTSSSVTAAGDLLFVTTSHGTNEKGQVVHPEAPSFLALDKHTGELVWADH
                     SPGDRIMEGQWSSPAFAVIGDVPQVIFGGGDGWVYSFLAERTATGQPVLLWKFDANPK
                     KTKYEEAGMGDRNYLVAAPVVAEGRVYVVTGRDPQWGEGPGDLWCIDPTKRGDVSAEL
                     VVDASGRIVPPRRTFAVDETAGEQVIPNPNSAVIWHYRGQDLNNNGKIDFEEEFHRTI
                     GMPAVANGLLVVGDHSGVVHCLDAATGKAFWTYDMMASIWGSPLIADGKVYIGDEDGD
                     VAVFALSRERQLLAENTVGSSVYTVPAATDGVLYIVSQRHLMAISQKKPVPSSEGP"
     gene            7174..8433
                     /locus_tag="PLD05_02160"
     CDS             7174..8433
                     /locus_tag="PLD05_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76262.1"
                     /translation="MTRSVLRLCLVLGLAMISLAIASVTCAADWYVAPDGRPNNQGTL
                     NSPWDFGSALDGTHKIAPGDTVWIKAGTYRHPDRSLNSPGFVVRLQGTEEKPIHVRAM
                     PGHRVTVDGGLSVVAPSDYLWIWDVEILVSENFTMSREINEPGSHPQSYGRPWGGLNI
                     HAGKGCKYINLVIHDNAQGVSFWRGATDSELYGCIIYDNGWKAPDRGHGHAIYTQNEL
                     GVKTIADCIMTGGYGYTMHAYGSENAYVDHYLVVGNIAYDGGTFLIGGGRPSKGIRVH
                     DNILYNVSLQLGYSAPYNEDCEVLNNLIIYGGLAINRFRSVREEGNVIVPRGTKSAPP
                     TPQVFVRPNLYDPNRGHVVVINWSKSTEVEIPLGKLAKRGYRYGLYDPRHLFDPPALE
                     GRIDGDTIRVPLSGDFAVWVLKVEETK"
     gene            complement(8468..9145)
                     /locus_tag="PLD05_02165"
     CDS             complement(8468..9145)
                     /locus_tag="PLD05_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOP76263.1"
                     /translation="MELTVLDILQSCPLFSEVDETGFRRLFAVARLVRFRKGQTIFLE
                     GQPCPGMYIVGNGLVRVFKRGSQGQEKILHLVGPGETFAEVAALGQFNCPASAEAVSA
                     TTCAFLPMDALQRLIMEDHDLCRQMLVGLCVWVRRLVDLVEDLTLRDATGRVARFLIR
                     QLDGKQNTVKLPGLKRHLAAQLDLTSETLSRTLRRLETRGLIRHLSRAQLQILKPDEL
                     MRLAELQ"
     gene            9423..10715
                     /gene="hcp"
                     /locus_tag="PLD05_02170"
                     /gene_synonym="priS"
     CDS             9423..10715
                     /gene="hcp"
                     /locus_tag="PLD05_02170"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOP76264.1"
                     /translation="MFCNQCEQTANCTGCVISPGVCGKNEDVQSLQETLLYGLKGMAA
                     YAHHARRLGMVDEAVDSFMEEALFATMTNVNFDLDSLFELVLECGRHNLRVMEMLDEG
                     HVKRYGQPSPSLVKEGTQAGPGILITGHDLRDLEELLAQCEGTPVKVYTHGEMLPAHM
                     YPKLREHPNLAGHYGGAWQKQRREFDEFSGPIVATTNCILIPKESYKDRVFTTRVTAV
                     PGGVRLKDGDFSPVIKKALECPPLPDNIVRTSTVGFHHNVILGLADTIVQAVKNGEIS
                     RFFVIGGCDGAEVGRNYFSDFARLTPPNSFILTLGCGKYRIREYDYGTLLGLPRLLDM
                     GQCNNAYGAIKVALGLAEAFGCSVNDLPLTLVVSWFEQKAVAVLLTLLYLGVKGITIG
                     PKPPAFITPKVFKILQEKYDLRLIGSSAEEDLKRVLAA"
     gene            10927..11547
                     /gene="nrfH"
                     /locus_tag="PLD05_02175"
     CDS             10927..11547
                     /gene="nrfH"
                     /locus_tag="PLD05_02175"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOP76265.1"
                     /translation="MNQAMQAAIASSRQFIRALLRLAWFPPGLRLLMFAVFGVCVGLG
                     ALAVHTSRAPSYLSDEPEVCVNCHVMRPEYVSWRHSSHAEVAHCNDCHVPHDSFVNHW
                     LFKARDGLWHATVFTMRWEPQVIHLSERAVPVVESNCRRCHERLVACTSLREHTAGDL
                     RCWDCHGDVPHGETRGLASSPGYLDPSLPPVGPRSKGVTIGGRPPR"
     gene            11647..13056
                     /locus_tag="PLD05_02180"
     CDS             11647..13056
                     /locus_tag="PLD05_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOP76266.1"
                     /translation="MSEAKGRQENSLMWIGWAVFFVLLIITFALGLLVSSIVTRRKEA
                     IPLPLPRPIARWESDSRKWADAFPREYDRYRMMMESSTKTKYGGAFPRDYLEETPANV
                     ILFAGYAFAKDYLQARGHVYAIHDVTHTKRVNEKTPATCWTCKSTDVPRILAEMSPAE
                     FYKQPFSAFKDQITHPIGCLDCHDPTTMRLTITRPALREALQRQGRDLENIPHQEMRT
                     LVCAQCHVEYYFKGEGKYLTFPWDKGFRIEEIESYYDEAEFSDWTHAISQAPMVKLQH
                     PDYEVYLTGIHAYRNIACADCHMPYRSEGGVKFTDHYIQSPLLNIANSCAVCHRWSEE
                     EIRQRVESIQDKTKEVRQRVEETLCKAHFEVAAARQAGATDDELQNPRQLIRKAQLRW
                     DFVAANNGMGFHSPGECLRILAAALDLASQARTELARILATHGVIKPIIYPDISTKTA
                     AQQVEQSFVNGNPPNLLPK"
     gene            complement(13136..13981)
                     /locus_tag="PLD05_02185"
     CDS             complement(13136..13981)
                     /locus_tag="PLD05_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOP76267.1"
                     /translation="MKSANDRREGDVEYEFGIPIPGRILPEDQWARTALKKLPPEGPI
                     DWAQVFGRTAPLILDIGCGNGRFTLASAIRRPEFDHVGIDILPVVIRYATRRANQRGL
                     WNVRFAVCGGHEFLDRYIAPHSVQEIHVYHPQPYKDPRKQHKRLLSPEFLALVFRSLF
                     PDGLFVLQTDNAAYWRYVRPLMDKFFNFHDQEGPWPDMPEGRTRREIYARLKGLPVFR
                     GYGTPRTDLSDTQAMELAKQLPPPVFSAAEKADPLAVAGRPRRSTARSFRQKRAEKWR
                     KRRNS"
     gene            14127..15335
                     /gene="chrA"
                     /locus_tag="PLD05_02190"
     CDS             14127..15335
                     /gene="chrA"
                     /locus_tag="PLD05_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOP76268.1"
                     /translation="MTTELEYGITCRAKMIIKLCQIIWTFAQIGLVSFGGPAASIAIM
                     QRTIVDRRKWVTQSEFLDFVAVSHMIPGPIAVQLSLHLGYRRGGLLGALAAVFGFVVP
                     AAFITWAVAVFYQRSHALPALEACLAGVRPAVLAVILISLWDIAGRLTWNKWRFVLLA
                     AVVGLNLVGIDAIVIMALTLAYSAFYLSVTGRKSRGQGKAVETNSEKGQPPAQGPNLF
                     GIFLPTAAAFSKAGLVGKSLYLFGMFFKIGLLLYGGGNVVAAYIQSDFVDRGLLTQAQ
                     FLDALAIGQSTPGPVLTVATFIGYVLAGHLGAIAATLGIITPCVILASLVHPWVVMAR
                     SSEKIATFLNVINLVVIGLILSVCLNLGIWVFQDFKAIGFAVVAGVLLWFKVPPVLVV
                     IAAAVAGWLF"
     gene            15795..18356
                     /locus_tag="PLD05_02195"
     CDS             15795..18356
                     /locus_tag="PLD05_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOP76269.1"
                     /translation="MGNLTTEYSHRGFFARYNLIILCAVTFAIPFMWIGAERALLSNK
                     NDVKSWLPDTYEETATFEWYRNHFESDMFILASWEGCTLDSPKLDLLARRLALDIQRS
                     SDDPYRFFKSVITGPQLINQLVEDQGLTREQAINRLRGFLVGQDGKQTCLVLVIDPEA
                     EARWEKHFGKNPRKKFLHAVLDSVYAAAKEICDIDPDELHLGGPPVDNVAIDVEGQRS
                     LVRLAIVCGAVGGLMAWWCLRSVVLTLLVFSTGVYSAGLSMILVWLTGTEMNAILLTM
                     PALVYVAAVSGAIHMANYYRDTLRTHEQWGLPDPAVGAPEKALKSAFLPIFLATFTTA
                     FGLASLSISELVPIQTFGIFSAAGIIVSFLFLCLYVPAMLQEWPVRNITKPRRTAFFD
                     PGRWSGWEHVGEIILRRGSWVVAGCILLMVLGAIGASQITTSVKLMRLFSPKARIIQD
                     YAWLEEKLGPLVPMEVVIKVDRDRAKLDLLDQIKLISEVQHAIQQLPDVGSVVAVPTF
                     ARPLPSRPGLVERRTWIVQLQRHREDLRDYWSEEESCELWRISARVKALTDLDYGVFV
                     NDIRQVVEPVLAKYRARSADGVTATYTGLVPLVYKAQHSLLNGLLTGFAGDLVLIAVA
                     MVFVMRDWSAGLLLALASVFPIAVVFGAMGWLGIVVDVGTVMTPAVALGVTVDDAIHF
                     MLWCRHGQERGMNRHESIMFAYRDCAQAIYQSWGVIGLGLSAFALSSFSPTQRFGYLM
                     LAMLTASSFGNLVLLPALLASPLANYFWRSGAKQAERKRSQLKTQKAPDLHPHVIPLE
                     QPAFEEEPVGVCATSEGVIYLETGGDGVTPSFPSERDCPQRWSGARKHRLPHKPR"
     gene            18605..19642
                     /locus_tag="PLD05_02200"
     CDS             18605..19642
                     /locus_tag="PLD05_02200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOP76270.1"
                     /translation="MAQKASTREIPYLAQRSRKGVITGVQVLGLGSYLPETRVRNEDL
                     VKLGCDPEWIIQRTGILERRHAPPEMATSDMAVEAARRCLLSAQVNPKEVDLVVFGTY
                     TPDMTMPATASLVQEKLGLFAPAMDIQAACTSFMFALITGMQFVGTGCAKRPLVIGAD
                     CNSRVCNPNDPKTFPIFGDGAGAVVLGPGDKDQGLIAFSYGSDGAGFDLLYRPAGGTR
                     TRKDEPGQLVDDGYLVMEGRPVFKWAVRMLNQTVHDVLEMADLTLAEIKLVIFHQANL
                     RIIKAAAETIGIPEEKLYNNLDRYGNTSSASIPICLDEAVREGRIERGDLVLLSGFGG
                     GLSWATGILRW"
     gene            19904..20695
                     /locus_tag="PLD05_02205"
     CDS             19904..20695
                     /locus_tag="PLD05_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76271.1"
                     /translation="MKAWRLVALVAVIVAGMTYASETSPPSLPAFKVESRVDLGEAEK
                     AVTSSITIFSRTKVFDFLEKPAEVTIIDWRDQRVVLLNPLHHEQTELSFDTIFRFQER
                     VQARALQDKDPRQRFFANPAFTIRMDSTEKELVFASNWLTYRVKVSDTVDPALIREFL
                     RFSDVMAQLNCLLVKGSRLPHPRIKVNKTLLERNLFPEEVILINGPRNFFEWLPGRRS
                     IVRSTHHLVLELTPQDRARLTQAEEWTQTFRRVNFIDYQAKLAAE"
     gene            complement(20735..21520)
                     /locus_tag="PLD05_02210"
     CDS             complement(20735..21520)
                     /locus_tag="PLD05_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76272.1"
                     /translation="MIRERMTNGRQLSLGRLFIGILCATFFVGSWAALVRGESQSSAQ
                     PSSPTKSAEEKKESATSPYKWRDLFDGKSLKGWKVPKFGGEGEVRVQDGCIVMERGDP
                     MTGIAWEGEFPKVNYEVEYVAKRTDGIDFFATVTFPVGDTFCSFVTGGWGGSVIGLSS
                     INFNDASDNETTQFMVFKDNQWYKFRVRVTKVKIEVWIDDKKVVDLVIGDRRISTRYE
                     VDLCKPFGFASWCSTGVIRSIRCRELTPEEVKAIEEEAEKTRL"
     gene            21945..22985
                     /locus_tag="PLD05_02215"
     CDS             21945..22985
                     /locus_tag="PLD05_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP76273.1"
                     /translation="MLISPQISKKALAQLCRRLATQTEAGLEIRTICRREAQRATGWI
                     NRRVFSQISQAVDHGQSLSDAFDATNDFFPPLFREIVRIGEQSGHLPEAFRLLAQYYE
                     EELHRRRTFWMLLAWPLFELSLAVLVIGFLIWIMGTISTQSGMTIDPLGFGLIGTRGL
                     AIYATFVAACIVTVIFLIWAIRRGMLWTQPVQYLVDMLPVLGEIYRTLALARMAWALS
                     IVVRSSMDVRSAVDLALQAANHLRFTAIRPRVWRSLQAGKSLYLTFAEAEIFPQDWLD
                     VLRVGEESGKLDEALSHLSRVYADRAAQAMKILSVVGAVAVTLLIAGVIIFLIFRLAL
                     FYIGQIQSALPA"
     gene            complement(23016..23585)
                     /locus_tag="PLD05_02220"
     CDS             complement(23016..23585)
                     /locus_tag="PLD05_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOP76274.1"
                     /translation="MPVSVSLGGCVVELVQGDITEQQVDAIVNAANSRLAGGGGVDGA
                     IHRRGGPEIMAETRAKYPEGCPTGSAVISGAGRLPAKYVIHAVGPIYRDGQHGEAELL
                     ASAYRKSLSLAIEHGCQSVAFPALSCGAYGYPVEEAAKIALGTTMEFLRQHGKPALVR
                     FVLFGQSTYDTFLKALKDLVGQDAVTERT"
     gene            23983..24849
                     /locus_tag="PLD05_02225"
     CDS             23983..24849
                     /locus_tag="PLD05_02225"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP76275.1"
                     /translation="MRRREFLAAAGAGVALSTFPLRWTHGAFSAPKKILYFTRSAGFE
                     HSVVARKGGELAFSEKILIQWGKEVNVEVVCSKDGGVFDGDLDQFDAFAFYATGDWTK
                     PVDRPNEPPATESGVKRLLEAIAAGKGVIGFHAANDAFHSRGNEISPFIKMIGGEFAS
                     HGRQQEATMRVVSPKFPGCEKLGDSFRMMEEWYAMKNLADDLHVILVQETEGMQDACY
                     QRPPYPATWARMHGKGRVFYTSLGHREDVWESSIMKQISLGGLAWVLGEVNFDPVPNI
                     KEVTPGANQLPK"
     gene            24970..25941
                     /locus_tag="PLD05_02230"
     CDS             24970..25941
                     /locus_tag="PLD05_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76276.1"
                     /translation="MSFAALSRRRFGQIVAGAASVAVFTCEPLQAKRIPEVTSPLEAF
                     RFCLNTATIRAQRLPLAEEIDLAAKVGYHGIEPWTREIEEYISSGGKLADLRKRLQDR
                     GLSVESAIGFPTWMVDDDTQRAKGFEQMKREMDWIAQLGGTRIAAPPAGAYNSPPIDL
                     RRVAERYAQLLDLGHSMGVVPQLEIWGASANLSRLSEAAFVLVECGHPDASALFDVFH
                     IYRSGSPVAGLRVFNGRNLHVFHMNDYPADPPREKIRDSDRVFPGDGVAPLSTILQIL
                     AEIGFQGTLSLELFNPEYAKMTAEEVAQTGLAKMKNAVLTAFQKESS"
     gene            complement(26039..>26923)
                     /locus_tag="PLD05_02235"
     CDS             complement(26039..>26923)
                     /locus_tag="PLD05_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOP76277.1"
                     /translation="VDMLKDIAEGEGDLTKRLPVTGNDEIAELAKWFNVFIGKLQEMI
                     HQVVVSADQFTEGARVVAESSQTVAQGAQTQAASVEQMTASIEELVRSVDSIRQLASE
                     ADQLARRSSQSAEHGGVAVEKSISAMNAIRESSKRIGDIIQVISEIAGQTNLLALNAA
                     IEAARAGEHGMGFAVVADEVRKLAERSNRAAQEIAQLIRESTRLVEEGAQMSDTLGES
                     LRDILDHIKETAAKVTAIAEKTTEQAAVAQEVRNAVQGIAQVAEQSAAGSEEMASSSE
                     ELGAQADALRQLVAGFKT"
     assembly_gap    26924..27023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27024..27964)
                     /locus_tag="PLD05_02240"
     CDS             complement(<27024..27964)
                     /locus_tag="PLD05_02240"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOP76278.1"
                     /translation="MKLQTKVVGFSVIGVLLTATVTTGLVAVNKSRLGEQVRTEIDAL
                     GKDTCRHIALNVREMLEVYHKALLKRLDQSMEAAKEMVGRSGDFTIKEEKVTWQAKNQ
                     FTGQVQNVELGKMYLGDAWLGQVKDLKEKAPLVDEVGKLTGLTCTVFQRMNSQGDMLR
                     VCTNVPGADGGRAIGTFIPATNPDGTPNPVVSTVLRGEAYVGKAVVVNDWYITKYEPI
                     FNDKKEVIGCLYVGIRRDDVPELRQSIMDITVGKTGYVYVLQGTGEKRGTYIISQGGK
                     RDGECIWEAKDADGHYFIQSIIEKALKLKPGECAFER"
     gene            28519..>29039
                     /locus_tag="PLD05_02245"
     CDS             28519..>29039
                     /locus_tag="PLD05_02245"
                     /inference="COORDINATES: protein motif:HMM:NF026192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOP76279.1"
                     /translation="MPGDIRKAAILLMSLPREQAAQVLARLDPKQVEAVTIEIARLGT
                     VTPDEQEQALKEFSQANPAGLTGGKGGLEVAKELIEQALGKAANATLETIKQSIEALP
                     FAFLQKVDSQNLITFLIDEHPQTIALVLSHLNPSQAAEIIRGLPPERQVAVVKRIAMM
                     GQTSPDIIHEVEKG"
     assembly_gap    29040..29139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29140..31737
                     /gene="mutS"
                     /locus_tag="PLD05_02250"
     CDS             <29140..31737
                     /gene="mutS"
                     /locus_tag="PLD05_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOP76280.1"
                     /translation="QYMEAKRACPDALLLFRMGDFYELFYEDAKIAAQTLNLTLTSRD
                     KSDNAAPMAGFPHHQLDSYLAKLLAAGFRVAVCDQVEDPRTAKGLVRREITRIVTAGT
                     VTEEGLLDPKEPNYLAAVVPQDPAGIAWIDLSTGRFYAGIFPASRLADELVRLRPAEV
                     LWPEDATQSVPAIDGMVVTKRPTWAFAAAHTQPILQRHFKGQALEGFGFESQGNDLPA
                     LCAAGAILDYLNETQRTTLAHIERLLRYRVGDFMEIDESTWRSLEVTRTIREARREGS
                     LLWVIDRTITGMGGRLLIDWLTNPLLDQKAIVKRQDAVAEFLNRPEICSKVRQALARV
                     FDLERLLGRATTGRCTPRDLASIARTLRQIPPLKEILAKCYGQRLGEIAQLLDPCPEL
                     EAKLSAALVEQCPLSATEGGFIREGYHAELDELRRLAQGGKQWIAAYQAKEIERTGIP
                     SLKVGYNKVFGYYIEVTNTHRDKVPADYFRKQTLKNAERYITPELKEYEEKVLSADER
                     AKQLEYEIFVELREFLLQFRSTLQRTAALLAELDVLAGWAELARERNYCRPELCEEPI
                     LEITEGRHPVLDITQPEGTFVPNDLRCGPEGFIILITGPNMAGKSTYIRQTALITILA
                     QIGCFVPAKSARIGIVDRLFARVGASDELARGRSTFMVEMIETARILNSATKRSLVIL
                     DEIGRGTSTYDGMSLAWAIVEYLHNRIGCRTLFATHYHELTELAEVLPGVKNFNVAVR
                     EWQDQVIFLHTIVPGAADKSYGIHVAQLAGVPRVVIERARQILEDLETKHLEGPNPFR
                     QVTSSSSSGQGRPIQLTLFEPIDHPLMEEIRELDLTKITPLEAFEKLRKWQEQLASRP
                     TKRRGWRST"
     gene            32011..36003
                     /locus_tag="PLD05_02255"
     CDS             32011..36003
                     /locus_tag="PLD05_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOP76281.1"
                     /translation="MHATLKGQDLAKLVWNELIRVVGLSLTWTTLFAIAMGADDRTFK
                     AFSLQHASPGQVESVLRSLVSPGTEIIQDTAGRRILISGPETDLQIAASVVAALDRPQ
                     ETSPQPPVAKPAVSQIKSYFVSRDRLQSMLAILQAQFANRSDVRITADERTSQILVLG
                     PQDVHAFVGGLLAQGPTPSQTGTTSQGRTPVSSSGSPANLPAGQAQQSGTGTSSSGRT
                     FPAQMTYASAEMNRDTSQMGSSLTIEEIPLWNLKATQLEAALQSMLGSTLEPINVVPQ
                     AARAEYQMALHTGEKLGLLVDRINHRIRVTGPMWSLMPARQLIQALDSPASSAGQTVR
                     LIPLANARSSTVHQAAQAIQAANSTQQEAENRLPQDLVVTQVEPSQPPTPQPSVTTTQ
                     EQPQGQQPSAPQPGVTQPPAVQAPPAEPPAPGEAAPGGAAPEEGGGLVGPVQIEFLEG
                     LDVLVVRGNRRDVERVMQIIRQIEEISVQTEPEIRIALLRHVDCEDLLAILTQVYQSV
                     YAARRGSITMIALVKPNAILLVGRQEAVNTVLELIAKLDRPVAPESEFKVFPLRHASA
                     TTIEQRLTTFYANRGGLGTKILITSDFRSNSVVVKASPRDMLEIEQLIAKLDTPTAEA
                     VNEIKVFTLKNSLASELAPILQQAISAQAAGALRQTATGQLTVQAGAQAQQEIRSTRL
                     RFVTVDTEGKQLLESGIMTDVRITADTRANALVVAAPAESMPLIEALITELDRLPSAE
                     AQIKVFTIVNSDAQALADMLQTLFGQAVTAAAGAAAQPAYRTGAQEGESSLIPLRFAV
                     DVRTNSIIASGSAGDLAVVEAILLRLDESEVIKRKTQVFRLKNAPANDVANAINSYLT
                     NERQVRQLNVNVFSTFEFLSKEVVIVPETVSNSLIVSATPEYFDEIVEIITQLDERPP
                     MVLIQVLIAEVQLSNTDEFGIELGIQDPILFDRSVAGVPGFLFNSTGALGNNTTAPGS
                     DIVGAQAISNFGVGRGNSTLGFGGMVLSASSDAVSVLIRALRQKRRLDVLSRPQVMAL
                     DNQAAFIQVGQQVPTVTGTSVTQYAQVNSVSYTNTGLILLVRPRISPDGQVVMEINAE
                     KSDVGPESEGIPISISAAGQIIRSPRINTTRAQTTISAGDGQTVILGGLITKSRSMTN
                     RRIPGLSDVPILRHLFRYDLGDASRTELLIFLTPHVVRTDEDVERARQIEAARMHWCL
                     ADVVALHEDLAPSPTSQSPTPAVPPGPAPQGIQPSQEAIPTPPPSLPNNQGQSQPRST
                     PGNANWNAVQQPVFFDTPMESPVDSKWQADVGTSHISDQPGRDNGAVVDENSQLAWPT
                     PAYPSSGTRR"
     gene            36064..37248
                     /locus_tag="PLD05_02260"
     CDS             36064..37248
                     /locus_tag="PLD05_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76282.1"
                     /translation="MRRPSSKPATISRQLPRMNYVVGMLLLSGLAITAVGCAKFDLRR
                     NIPWQETSDKPVRSVVAFWTEAVIERTSGPPYRGFGGRLLFYPADMSHPVKVQGTVVV
                     YAFDEDLPPPENLKPVRKFVFTNEQLQQVYSESKLGPSYNLLIPWEEFSPKSRRVSLI
                     VRFIPENGPSVVSEQVKVFLPGLDESMLAGNSRAASDRQPSPNSNSADPSLDAADGSR
                     TAVTQWPISPTSYQESVKQAQPESRMQDKGVSSSSGVTTPEKKRRMETLTLNISPRLG
                     NGVPSRLGHGDTSLSTGGNHEGAQSQQTSPVGHASPNTPGASASAQTQSQANLSSLNI
                     QTPEQAYLTTVSAKIPRSPGELIADLNRQPPARFAPYRPLVPEGGVSQGLTDRSPSAL
                     SP"
     gene            complement(37130..37930)
                     /locus_tag="PLD05_02265"
     CDS             complement(37130..37930)
                     /locus_tag="PLD05_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOP76283.1"
                     /translation="MTSLRTRLMAVVVGMALSLWGTLETAQAQTGYSSGLFGTQSFGS
                     SLTGQSSTRISGTSRSSSLGSSGLSTMSSGQFLNFQSVNQAEQFFGSSSFVGADSADI
                     VNPLSALQGSSSGRTTGLTGRTTTGLTGRTTLGSSSLMGLSSRGLLGSRSSSTYARSR
                     TSSRYGSRSQTQRDVLRSTVRVAFNPPVENSEQIAAELAARLSRIPGLKNAPQAANVQ
                     VDLEGRTAILKGTVPTANDRLVLERLLLLEPGVDKVQNELVVAGSNPQ"
     gene            complement(38476..>38675)
                     /locus_tag="PLD05_02270"
     CDS             complement(38476..>38675)
                     /locus_tag="PLD05_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76284.1"
                     /translation="ALRVEELPPAPACRVSVAVQREPEFILLQPQGMEIRTWKYESGR
                     IEFEIPGFDIHQMAVIKLKEE"
BASE COUNT         8619 a        10729 c        10248 g         8779 t
ORIGIN      
        1 tatcggtctt tggaagaagc aatacggctt tgccatcccc gagggtgatc gtaaggacgt
       61 aacctccgct ctgcatttcc aatttgacct gccccggcga ggccgatccc acgcgataag
      121 ggtgatagac tgtcagaaac tcgacactgc gttccggatt taccgtttcg gccgtcagat
      181 gccattctcg aatcttgacg cgaggacgag gattaggatc gtactcattc gtttgatgaa
      241 acttcaagtt ttgcggaacc aggaattgca cggggcactc cactttaccc accctggcaa
      301 ccaacccgtt tccgatgacc tccatcttct cggggctgtg cagccaatac tggtaggtca
      361 caggtcttcg cgcaaccaat cgatcataga caacgatcca atctggcttg acaaagacaa
      421 tcgatcgcgt gaaccgatcc aagagacgcc gatctccctg cggccctgcc ttcgggtcga
      481 ggtaacaact gcccgcctcg ccctcgacgc aatcgacctc gggcgaggta taaaaggccg
      541 tgatttcacc tcgcgttgcc gcggaatgcg gggtttggtc aaccccatcc accgtgatac
      601 tgttcaccga acgggtgctc cacatccatc gaacgtgatg gtcacttccg tagatgtcgc
      661 gtcgtccact ggacacgaac aacctctcat tgtaggccgt taacagaaaa gcgttgttgg
      721 cgtcgtaacc gtgagactga agcccaaacg gactcgactt aaagaggacc tgcacgtttt
      781 cgcgggcatc gagaagtgtt gtattcagag ccgcttgccc cgtcccccga aagacccgcg
      841 atgttggcaa gtccaccggc ggctttgacg aaactttggg cagcgtcccg cggatgaatt
      901 cccaaaactc tctgggctca ggcgctcccc ccatcgactc gacataccac atccaataag
      961 gattccgcgc ctggaccgct agaatcgaca tcaaaggcac attgcttcgt gattctcggg
     1021 tacatgccaa atcaccgaag cctccgctca caccgtgagg tggcatgagg tacatggcat
     1081 aataccccgc cttggaaaaa tagggttttc gataggcatc gattccaaat gtccctcgca
     1141 tcacgtcggc ccaccatgta aaccgttgaa tgtagctggc ccaatagctc gatccttcat
     1201 gccaaccacc gtcatcgtca caccacaccg gataggtatt gaagaagacg ttcatggcga
     1261 accaggtcca atcgtcggcc tcgggaatct cttgatgaaa agtcacgccc acctcgccca
     1321 gaaagtgcca tgctcgattg gcgtgactgt tgtaaggttg ccagaagtgt cgggggcata
     1381 aggtacgata catctcctcg ccacgtatct tcatgacttg ccgacactgt tggcgttcct
     1441 cctctgagag caagtcgtaa acaaacgtgt atgtccggca aaaatagtag gcatagggca
     1501 taccagcctc gtcgttgtat cggaaccccg ttgcccctac agggtcccac ttcgcgcact
     1561 ccatgagaat ctttctggca agctccccat aaggacgatg cccgccaact tgatagacaa
     1621 atcccagaag tgccgaagtg cccagcgcac gaatcgtgta ttcccgattt ccccaccaaa
     1681 ttttcgccca ttcgtcgctt tttctttgca tatctggagg gtatttaggc ggttcctccg
     1741 ttggcggcgg attgcgaaga gcctcttcac accgctggac caatctttcc cacacctccc
     1801 tttgctcgtt ggtagccgcc tgtttcagtc gggggaggtc ttcaggacga atgaacaatc
     1861 gcggatgctg tttgggaatt ctcgccagca ggtcttcccg cgtgggcatc ggataaggca
     1921 cggcgttggc cgaaatcgtg aatgaccgca cttgactcca ctgggttgga ccgcccttgc
     1981 catgaccgcg gtaacgccag aaataccgac ctgggccaag cgttcgcggg ggacagtgca
     2041 cgttccaaat cacatcttgg acccgataag cgatattttg aaattcacga tcccgcgcaa
     2101 cctcgatctg ccacttctcg acctcttttt gcggccgcca actgaatgcc ggaggatcca
     2161 ccatggacgt gctgccatct tccggccgat atccccactc gcccggctga gttggccgct
     2221 catccagctt caaccctgca ctttctccgc gcgccgtact gagataactg accacggcaa
     2281 ttaacagagt acaaacaaaa accaactttc gcatggaaca tgcctcccta gcatcgcttg
     2341 ctaacacaga caacttgctt ggccagtgct ggaacaaccc gaacaaacgg agacatccaa
     2401 taaaccaatg gcaggtagga atgttctaac ggacagggac cttcgtttca agacaacagc
     2461 aatacatcac aggacaaacc gcacacagtg gccacgaatc caaatcatag ctggtcaccg
     2521 cccatgaccc ctaacttatc tgccgggggt tccactcgtg aacggtctgg acaagcaacg
     2581 agacattact gaccggcgtt gagggtaaca caccatggcc taggttaaag atgtgcccag
     2641 gacgtccccg aacacgctgc aagattgccc gcgcctcgtg cacgacgagc tcctctgggc
     2701 cacacaacac gacagggtcc agattcccct ggattgcgta accgtcccct accacatccc
     2761 aggcatcacc aatgtcgatg cgccaatcga caccgatgac ggtccccccg gcctttgcct
     2821 ggagtggaag caacatgggg ttacctgtca ggaaatgaat tgcaggcacg tcggcgggaa
     2881 tggcttcgaa tactatttgc gtataaggca agacaaattg ttgataatgt tttgggctta
     2941 ggcacccagc ccagctatcg aaaatctgaa tcgcctgaac gcctgcggtc acctgctttt
     3001 taacatagcg ggcgatcgat ctggccatcc gttccattaa ttcccgccag gcaccgctgt
     3061 cttcaatcat gaaacatttt gtcctttcaa aagaccgcgt cgttcctccc tcgatagcat
     3121 aagccgctag ggtgaacggt gcaccggcaa acccaatcag tggaatcgtt ttaggcaagc
     3181 cctcccgagc caggcgaatg gcctcaaaaa caaagtccaa ctctgcaagg cgatcttccc
     3241 ccagctcttg aacgcggtcc acatctttgg ggccgccaag atgattccga atgacaggtc
     3301 cttcgcccgg gccaaatcgt agctcgaagc ccatcggctc gaggatgggc aacaaatcgg
     3361 cgaaaaggat cgctgcgtca aacccgaagc gtgctaccgt gctaatagtg atctctgctg
     3421 ccagctcggg cttccggcat agttcgagaa aagaatactt ctcccggagc gctcgatatt
     3481 ctggcagata tcgccccgct tgccgcatca accagatcgg agtccgctcc acgggctccc
     3541 tgcgcgctgc cttcaagaag ggactatcgt accatgcggg agtttcgacc gccatcgatt
     3601 accactcttg tcctgtgact ttgaaacgaa tcatagccca tcgtcgatga atgtcatctc
     3661 aatcttaacg tgcgtggtga ggggccgcca cgcccattgg tctatgctcg caggtgccta
     3721 gctcgccgtg ggcagacttc tttcgaagaa ttacgtgcgt ttcatgccca tgatacgcaa
     3781 taaaaaagac cgtccgaagc tttctcctgc ttcggacggt cggttgtaat ctttatgaca
     3841 agcctctcct tcacaacatg gctgatttca gctcatgcgt tgggtgaagg ggcagcgggc
     3901 aacggagccg gtgccgattt tccgcctttc tccaccgggg cagcctcaac gccacagccg
     3961 caatcgctaa cgacgcaagc ggagcggtcg cagccaagca ggttccggag gaaattcagg
     4021 acgggacgct tgggcgcgca ggaaggttcg caagccggtt cgcaagcagg ctcacaagcg
     4081 ggttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgag agaccgtggc
     4201 cttccaacct gtcggcccca acccgagcac cgcacacggc cgctgcgcga cgcgctcaat
     4261 ctcggtcagt gggttctccc atgggttaga accgactggg gtggattagg gtcgcagcgc
     4321 gggaaggttc gcacgcgtcc ctcaaacccc aaccaattgt cgtcaggacc gtcgaaccaa
     4381 tccttggctc gaatgatccc cctcggctcc ctgacgcacc tcttgctgac tacgttgaac
     4441 tcatgcacct cacatggttc aatgttggta atcggcagcc caagagacgc ggcttgaaca
     4501 cacatgacgc cagtcacaca aatggtttac tctgcggttt aagctccctt ttccttttca
     4561 aaagggtaca gagatgagat aaactatgcg gggtaaaaag gacggttgct tgtcattaac
     4621 tgttgcccaa tcgttagatt ttaacatgcc ggtaaacttt aactctcaga tggtcagctc
     4681 cctagcccgc aaaggcatga cttgcttccc atgctcttga aaaaggaaca tctcgatatg
     4741 ggtcaaaagc gttactacgt agtcgatttc gctcagttgc ctggtgttcc atgtccgtgc
     4801 gggacggcat atcgcgccct ggcagatgtt gacgcatttc cgggcaccat ccaccgcacc
     4861 gaaatctcag gggctgcccg cgcccattac cataaacggc taaccgagac gtattacatt
     4921 ctcgaggcaa ggcccggttc ctatttggaa ttggatggtg atacgattcc cgtacatcca
     4981 ggcgtgtgtg ttgttattcc gccgggggtg gtgcaccgca tcgtcgggga cgtcgtcatt
     5041 ctgaacattg tgattccaaa atttgatccc gcggacgaat accttgcaga acttccttcg
     5101 gaagattgcc gccgttgtgg cgatcatgat gggccaaccc ggtgatggca gtctggtcga
     5161 accgcacttg cactggagat aataacactc gcacaatgat cggggatttc ggcctgtccc
     5221 cggttaggtc gctaccgttc ctcggagcac gtttcgtaac tcagccatcg tcgatctttg
     5281 cccaaaaagg gggctaagca tgatccgcca tcttgatatg caaaaagacc atgggaacca
     5341 acggaccaga ggtttcattt tctcgtttgc atttgtcggg atagtgtggt tccttcaagc
     5401 cgcttcagtg attgctgctg attggccaca attgggcggt tctccaagcc gtaacgccgt
     5461 tgccatcacg gctgctttgc ccgtggaatg gaacctcgga gcaattgatc gggcgacctg
     5521 ggaatggaaa ggtggccctc aagctcaaaa catcctttgg gtcgcaccgt tgggaacaca
     5581 gtcctatggc tcgccagtcg tggcgggcaa gtacgtattg tgcggcacca acaacggtcg
     5641 tggatacctc gagcggtttc ctaaggaatg cgacctgggc tgtttgttgt gcttttccag
     5701 aacagatggt cgctttgttt ggcagtatgg agccgaagtg ttgccagatt ccaatcaaaa
     5761 ttggcccgaa cagggtcttt gctcgacgcc tttagtggag ggcaatcggg tgtgggtcgt
     5821 gtccaatcgg ggttgtgtgg tgtgtcttga cctcgaaggg ttccatgacg gggagaacga
     5881 tggccccttt gcgcaggaat ccagtaccgg tccgaacgac gccgacgtcg tgtgggaatt
     5941 cgacatgatt aaccgcctcg gcgtgtcgcc acgctacatg acgtcctcat ctgtcacagc
     6001 ggcgggtgat ctcctctttg tgacaacctc ccacggtact aacgaaaaag gacaggtcgt
     6061 tcacccggaa gcccccagtt ttcttgctct tgacaaacat acaggcgaac ttgtgtgggc
     6121 cgatcattcg cctggtgatc ggatcatgga aggccagtgg tcaagccccg cctttgcggt
     6181 gattggggac gtcccccagg tcatttttgg gggtggcgat ggctgggtgt attcctttct
     6241 tgcagagcgc acagcgaccg gccaacctgt gctcctttgg aaattcgacg ccaacccgaa
     6301 aaagaccaaa tacgaagagg ccggcatggg tgaccgaaat tatttggtgg cagcacccgt
     6361 cgtcgcggag ggccgagtct atgtcgtgac gggacgcgat ccacaatggg gtgaaggacc
     6421 tggcgatctg tggtgcattg atccaacaaa acggggcgac gtgagtgccg agttagttgt
     6481 ggatgcctcg ggcaggattg ttccgccccg caggactttt gccgtcgatg agactgctgg
     6541 cgagcaagta atccccaatc ccaattctgc cgtgatctgg cactatcgcg gtcaggacct
     6601 caacaacaac ggcaagattg acttcgagga ggagttccat cgcaccattg gaatgcccgc
     6661 ggtcgccaat ggactcctgg ttgttggcga ccattcgggc gtggttcact gcctggatgc
     6721 cgcaacaggc aaggcctttt ggacatacga tatgatggca agcatttggg gatcgcccct
     6781 catcgccgac ggcaaagtgt atattggcga cgaggatggc gatgtcgcgg ttttcgccct
     6841 ctcacgagaa cgtcaactgt tggcagaaaa caccgtaggg agttcggtct atacggtgcc
     6901 ggcagccacc gatggggtgt tatacatcgt cagtcagcgc cacctgatgg cgataagcca
     6961 gaaaaagcca gttccgtcgt cggaaggtcc ttaactttac ggatcagtca tctttaatct
     7021 taaggatcag ttaggggaac gatggccacc gctgcatgac gatgaccgat ttggcaattg
     7081 ctttacatcc ggagggtcgg agggaaacgt acgaacggaa caaaaaatat ggtaaaatgg
     7141 ggattttcgc actgcccacg cagttggtac gtcatgacca ggtcggtgct tcgtctgtgt
     7201 ctcgtgcttg ggctggcaat gatctcgttg gctattgcca gcgtcacttg tgccgcggat
     7261 tggtatgtcg ctccagatgg gaggccaaac aatcaaggga cactgaattc cccatgggac
     7321 tttggctcag ctttggacgg cacacacaag attgcccctg gagataccgt ctggatcaag
     7381 gctggtacct accgccaccc agatcgctcg ctgaatagtc cggggtttgt ggttcgactt
     7441 cagggtaccg aagagaagcc cattcacgtg cgcgcaatgc cgggccatcg cgtcacggtt
     7501 gacgggggct tgagtgtggt ggctcccagt gattacctgt ggatttggga tgtagaaatt
     7561 cttgtctcag aaaacttcac aatgtcccga gaaatcaacg aaccgggatc gcatccccaa
     7621 agctacggaa gaccttgggg tgggttaaac attcatgcgg ggaaggggtg taaatacatc
     7681 aatctggtta ttcacgacaa cgcccaaggc gttagtttct ggcgcggggc aacggatagc
     7741 gaactctatg gatgcatcat ctacgacaac gggtggaagg cccccgaccg tggccacgga
     7801 cacgctattt acactcagaa tgagctggga gtaaaaacaa ttgccgactg catcatgaca
     7861 ggcggctacg gctacaccat gcacgcctac ggttcagaaa atgcgtatgt cgatcattat
     7921 cttgtagtgg gtaatatcgc gtacgatggc ggaacatttc tcatcggcgg tggtcgcccc
     7981 agcaaaggaa tccgcgtcca cgataacatc ctctacaacg ttagcctgca actcggttat
     8041 tcggcaccgt acaacgagga ttgtgaggtc ctcaataacc ttatcattta cggtgggctt
     8101 gctatcaatc gttttcgatc ggtgcgcgag gaaggtaacg tcattgttcc tcgcgggacc
     8161 aaatcggctc cgcccactcc ccaagtgttt gtccgcccaa acctgtacga tcccaaccgt
     8221 ggccacgttg ttgttatcaa ttggtcgaaa agcacagaag tggaaattcc gctaggtaag
     8281 ctggcaaagc gcggctaccg ctatggcttg tacgatcccc gacatctttt tgacccacca
     8341 gctcttgaag gccgcatcga cggggacaca attcgcgtgc ccctttcggg tgacttcgcg
     8401 gtgtgggtgt tgaaggtaga ggaaaccaaa taaccgcgct gcaacagttg ctggaacaat
     8461 cgccgaatca ctgaagttcg gccagtcgca tcagttcatc cggtttgagg atttgcaatt
     8521 gggcacgcga taagtgtctt atcagaccgc gcgtttccaa gcgacggagc gtccgcgaaa
     8581 gggtttcact tgtcaagtct aactgcgccg caagatgccg cttgagacct gggagcttga
     8641 cggtgttctg ctttccatcc aattggcgga tcaaaaaccg ggccacacga ccggtggcgt
     8701 cacgaagcgt caagtcttcc actaaatcga ccaatcgacg tacccagacg cacagtccga
     8761 ccaacatctg ccgacaaagg tcgtgatctt ccatgatgag ccgttgtaac gcgtccatgg
     8821 gcagaaatgc gcacgtagtg gcactgacag cctccgccga ggccggacag ttgaattgtc
     8881 ctagggcagc gacctcggca aaggtttccc ctggaccaac caaatggagg atcttctcct
     8941 gaccttgcga tccccgtttg aagacccgca cgagcccgtt gcccacaata tacatccctg
     9001 gacatggttg accttcgagg aaaatggttt gcccttttcg aaagcgtacc agacgggcca
     9061 cagcaaacag ccggcgaaag cctgtctcgt caacctcgct aaagagcggg caactttgca
     9121 ggatatctaa caccgtcagt tccataccgt tcctcgtggg caatttttgg gaaaacttga
     9181 tctacgtcaa aaacctttcg cgccccagac ggtattatag tgttgaccga tcgatgacgt
     9241 gactgattcg gagtggctta cccgaacgtg tgaagtcgca agacgagact gagttaagac
     9301 tcttaccgtt atcaagtcac gattctgtat caggacgaac acggtcacta gtggtcggag
     9361 catcgatgtt acgaagcccg agaggcagtt tttggaaagc cctttgaaaa ggagagaatg
     9421 caatgttttg taaccagtgc gaacagacag ccaattgcac aggttgtgtc attagcccag
     9481 gtgtctgtgg caaaaacgag gacgtacagt cgcttcaaga gaccctgctg tatgggttga
     9541 aaggcatggc ggcttacgcc catcatgcgc ggcgattagg aatggtcgat gaagctgtgg
     9601 acagcttcat ggaggaggcc ctgtttgcca ccatgacaaa cgttaatttt gacctggaca
     9661 gcctgtttga gcttgtgctg gagtgtggcc gccataattt gcgagtcatg gaaatgctcg
     9721 atgagggtca cgtcaagcgg tatgggcaac cgtcgcctag cctggtcaaa gaagggaccc
     9781 aggcagggcc ggggatcctc attacgggcc acgacctgcg tgatctggag gaactgcttg
     9841 cccaatgcga gggtacgccg gtgaaggtct atactcacgg ggaaatgctc cctgcccata
     9901 tgtaccccaa gttgcgcgag caccctaact tggcaggtca ttacggcggg gcctggcaga
     9961 aacagcgtcg cgaatttgac gagttttctg gtcccattgt ggcgacaact aactgcatcc
    10021 tgattcccaa ggagagttac aaagatcggg ttttcactac gcgggtcaca gccgttccag
    10081 ggggagtccg gcttaaggat ggcgattttt cgcccgtgat caagaaggct ctcgagtgcc
    10141 cgccccttcc ggacaacatt gtccgcacaa gcacggtcgg cttccatcac aacgtcatcc
    10201 tcggattagc tgatacgatc gtgcaggccg tgaaaaatgg tgaaatcagc cgcttcttcg
    10261 tcattggcgg gtgcgatggt gctgaagtgg ggcgaaacta ctttagcgac tttgcaaggc
    10321 taacaccgcc aaactcgttc attctgacgt tgggttgcgg aaagtatcgg attcgggaat
    10381 atgactacgg cacgttgctc gggttgcccc gtctgctgga tatgggacaa tgcaacaacg
    10441 cctatggagc cattaaggtc gcactggggc ttgcggaggc cttcggttgt tccgtgaacg
    10501 acctaccgct gactttggtc gtctcgtggt ttgagcaaaa ggctgtggcc gtccttttga
    10561 ccctgctgta cctgggcgtt aagggaatta ccatcggccc gaagccgccg gcctttatca
    10621 caccgaaggt gttcaagatt ctgcaagaga agtacgatct tcggctcatt ggaagcagtg
    10681 cggaagagga cctcaagcga gtcttggcag cataaagatt ggctggcgtc attggcctac
    10741 agcacaccgc tgtgccaagg aactcagcaa gctggtacaa catcggcctt cgcctaaata
    10801 ggcagttgag tgacaagtct cattgagtta gaatctgctg tgattgccgg ggccacttgc
    10861 gatacaactt ctcggtgagt ggcccttggc gtgttttcga aggtctctta atcgagggaa
    10921 aagacggtga atcaagcgat gcaagccgcg atcgcctcca gtcgtcagtt catccgggca
    10981 cttcttcggt tggcatggtt tccgccaggt ttgcgattgc tcatgttcgc agtcttcggt
    11041 gtttgcgtgg gtctcggggc cttggcggtt cacacatctc gggcaccatc ctatttaagc
    11101 gatgaacccg aggtgtgcgt aaactgtcac gtcatgcgtc ctgaatacgt cagttggcgt
    11161 cacagcagcc atgcggaggt ggcccattgt aacgattgcc acgttcctca cgattcgttt
    11221 gtaaatcatt ggctattcaa agcacgggac ggcctgtggc acgcaacggt ttttaccatg
    11281 cggtgggaac cccaggtcat ccatctgtct gagcgagcag tgcctgtggt ggaatcaaat
    11341 tgccggcgtt gtcatgagcg gcttgtcgcc tgcacatcac ttcgtgaaca caccgcaggc
    11401 gatcttcgct gttgggactg ccatggggat gtgccccatg gggaaacacg cggactggca
    11461 agttccccgg ggtacctcga tcccagcttg cctccggtgg gtccacgatc gaaaggcgtc
    11521 acaatcggcg ggcgacctcc gcgataggct ttttgcaaga aagagcgtaa acttgcctca
    11581 attgacgaag aatccgtgat ttattaactg cgagaagaca catttcgctg cgagggatgt
    11641 aaaaccatga gcgaagcaaa gggtcgtcaa gaaaacagct tgatgtggat tggttgggcg
    11701 gtgttctttg tcctgcttat tatcaccttt gccttgggac tacttgtctc atccattgtg
    11761 acacggcgca aagaagccat ccctttgccc ctaccgcgtc ccatcgcgcg ttgggagtcg
    11821 gacagtcgca aatgggccga tgcttttccg cgagaatacg atcgctatcg gatgatgatg
    11881 gaatcttcta cgaaaacgaa atacgggggt gccttccccc gcgattactt ggaggagact
    11941 ccagccaatg tgatcctctt cgccggttac gcctttgcca aagactatct tcaagcccgc
    12001 ggacatgtct acgctatcca cgacgtgact cacacaaagc gagtcaatga aaagacgccg
    12061 gcaacatgtt ggacctgcaa aagcacggac gtcccccgga ttttggcgga gatgtctcca
    12121 gcggaatttt acaagcagcc gttctcggct ttcaaagatc aaatcactca tcccattggc
    12181 tgcctcgatt gtcatgaccc aaccaccatg cggttgacca ttacgcgacc cgcgcttcgc
    12241 gaggcgctac aaagacaagg acgtgatctg gaaaatatcc cccatcagga aatgcgaacg
    12301 ctggtgtgtg cccagtgcca tgtggagtac tacttcaaag gcgagggtaa atacttaaca
    12361 tttccgtggg ataaaggctt ccgcatcgaa gagatcgaat cttattacga tgaggccgag
    12421 ttcagcgact ggacgcatgc gattagccag gcgccgatgg tcaagttaca acatcccgat
    12481 tacgaagtgt atctcaccgg gattcatgcc taccgaaaca tagcctgtgc cgactgccac
    12541 atgccttatc gcagcgaagg cggcgtgaaa ttcaccgacc attatatcca gagtcccctg
    12601 ctcaacatcg ccaattcctg cgccgtgtgc caccgttgga gtgaagaaga gattcgtcag
    12661 cgggtggaaa gcattcaaga caagacgaaa gaggtacgtc agcgggtcga ggagaccctt
    12721 tgcaaggccc atttcgaggt cgccgccgcc cggcaggctg gggccacaga cgacgagctg
    12781 caaaacccgc ggcaattgat ccgcaaggcc caacttcgat gggattttgt ggcagcaaac
    12841 aacggaatgg gtttccattc accgggtgaa tgtctgcgaa tcctggccgc cgccttggac
    12901 ctggcaagcc aggcaagaac ggaattggct cggatccttg ccacgcatgg cgtgattaaa
    12961 cccatcatct atcccgacat ctccaccaag accgccgccc aacaggtgga gcaaagcttc
    13021 gtcaacggca acccacccaa cctattgccg aaatgatcgc acgcgcgact tggctaggcg
    13081 aaacattaac ctcctggctt ggtcgcaggc tgcgaggtgt ttgatgcacg acaggttacg
    13141 aattacgccg ttttcgccac ttttccgctc ttttttgacg aaacgaccgg gccgtggatc
    13201 gtctcggccg accggcaacg gccagaggat cggctttttc cgcagcagaa aagacgggag
    13261 gaggaagctg tttggctaac tccatggcct gggtgtcgct caggtctgtg cgcggggtac
    13321 cgtatccccg aaacaccggc aaccctttga gtcgcgcata gatttcacgt cgggtccgcc
    13381 cctcgggcat gtccggccag ggtccttctt gatcatggaa gttgaagaac ttgtccatca
    13441 gtgggcgaac ataccgccaa taggcggcat tgtctgtctg tagcacaaac aaaccatctg
    13501 ggaataagga gcgaaagaca agggcgagaa attctggcga gaggagtcgt ttgtgttgct
    13561 ttcttggatc cttgtagggc tggggatggt aaacgtgaat ttcctgcacg ctatgtggcg
    13621 caatgtaacg atcgagaaac tcatggccac cgcacacagc aaaacgaacg ttccataatc
    13681 ctcgctgatt tgcgcgacgt gtagcatatc gaattaccac cggcaaaata tcaataccaa
    13741 cgtggtcaaa ctccggccgg cggatcgcac tggccaaggt aaaccggccg ttgccgcatc
    13801 caatatcgag gatcagaggt gcggtacgcc caaacacttg tgcccaatcg atgggtccct
    13861 ctgggggcag tttcttgagg gcggttcttg cccactggtc ctcgggcaaa atcctgccag
    13921 ggatcggtat cccaaactcg tattccacgt caccttcgcg acggtcattg gcacttttca
    13981 taaggggtca ctccatccca ttggcgatgc gccaatgatg tcctacaatt gctgccattg
    14041 tcccaggccg aatcacgcgc cgggaaaatc aatacttcat cgcaaagacc aggcaaaaca
    14101 caacctccgg aggctttttc caaacgttga ctaccgaact cgagtacggc atcacgtgcc
    14161 gagccaaaat gatcatcaag ctttgtcaaa tcatatggac gttcgcgcaa attggcctcg
    14221 tcagcttcgg tggtccggct gcttccattg cgataatgca gcggaccatc gtcgatcgcc
    14281 gtaaatgggt gacgcaatcg gaatttcttg actttgtggc cgtcagccac atgattccgg
    14341 gtcccattgc ggttcagctt tctctccatc ttggctaccg gcgtggcgga ctgctaggag
    14401 cactggccgc agtgtttggc tttgttgtgc cggctgcatt catcacctgg gcggttgccg
    14461 ttttctatca gcgtagccat gccctgcctg cactggaggc ttgtctggcg ggcgttcggc
    14521 ctgccgttct cgctgtgatc ctaatttcgc tttgggacat cgcgggaagg ctcacttgga
    14581 acaagtggcg atttgtactg cttgcggccg tcgttggctt aaatctcgtg ggaattgatg
    14641 ccattgttat catggcactc acccttgcgt acagtgcgtt ctacttaagt gtgacgggtc
    14701 gtaaatcacg tggccagggc aaggccgtgg aaacgaattc ggaaaaaggc cagcctcccg
    14761 cccaaggccc caacctgttt ggcatatttc ttccgaccgc agcagcattt tccaaagcag
    14821 gccttgttgg gaagtcgctg tatctattcg gaatgttctt caaaatcggg ctcctcttgt
    14881 atgggggagg caacgtggtg gccgcttata tccagtcaga ttttgtggat cgtggtcttc
    14941 tgacccaggc ccaatttctt gacgcccttg ccatcgggca gagtactccg ggccccgttc
    15001 tgactgtggc aacattcatt ggttacgtgt tggcaggcca tttaggggcc attgccgcta
    15061 cacttggcat cattacacca tgcgtcattc ttgcaagcct cgttcatccc tgggtggtta
    15121 tggcccgaag ttcagagaaa attgccactt tcttaaatgt catcaatctc gtagtaattg
    15181 gtctgattct ttcggtttgc ctgaatttgg ggatttgggt ttttcaagat ttcaaggcaa
    15241 tcgggtttgc ggtcgtcgct ggcgtgttgc tttggttcaa agtcccaccg gtcctggtcg
    15301 ttatcgcggc agcagtcgcg ggatggttgt tttgatgcga agatttcact cggttgcagt
    15361 tggcgccatt gatgcataga attaaaccag gttggtcata atgaagtggg ctagggtgct
    15421 gcttccgttt tgtggggctt tgccttactg aatacgaaga aaaaggacac cgtcaagcga
    15481 ttacggtttt accaatcgtg tcgctttgct ttggtagttg ttttgtagga acgattccga
    15541 ccgaattcat tcacttcccg tcttacctgc tgaatcggcc gaattattga aaaaggcgct
    15601 gtgacctgcg gcctcgtctc caagatctgg gagacgctca aaaccttgac cgactcatag
    15661 gagttttctg atttttgtgg aacgacatcg ggtacggtcg aaagcgagag ccgttattcc
    15721 ggatcttcat tccagagacc aagagataca ccggattggg ggatacatcc cgggattgag
    15781 aaggattact tttgatggga aatttgacaa cggaatattc tcatcgcggc ttttttgccc
    15841 gctataacct tatcatcttg tgtgccgtta cattcgcgat cccgtttatg tggatcgggg
    15901 cggagcgggc gctacttagc aacaagaacg acgtcaagtc atggctcccc gatacttatg
    15961 aggagaccgc cacttttgag tggtatcgta atcacttcga gagtgatatg tttatcctgg
    16021 caagctggga gggctgcacg ctggatagcc ccaagttaga cctgcttgcc cggcgactag
    16081 ctttggacat ccagcggtcc tcagatgatc cctaccgttt tttcaagagt gtgatcactg
    16141 ggccccagct catcaatcag ttagtcgagg atcagggtct cactcgggag caggcgatta
    16201 atcgtcttcg agggtttctt gtcgggcaag atgggaaaca aacctgtctg gtcctggtga
    16261 ttgatccgga ggccgaggca cgctgggaga agcacttcgg taaaaaccca cggaaaaagt
    16321 tccttcacgc tgttcttgat agcgtctatg ctgccgccaa agaaatttgt gatattgatc
    16381 cagatgagct tcacctgggc gggccccctg tggataacgt tgcgatcgac gtcgagggcc
    16441 aaaggtctct tgtccgccta gcgattgtgt gcggcgctgt aggcggcctt atggcctggt
    16501 ggtgcctgag gagtgtggtt ttgaccctcc ttgtcttttc cactggggtt tacagtgccg
    16561 gtcttagtat gatcctcgtg tggctgacgg ggaccgagat gaatgccatt ctcttgacca
    16621 tgccagcctt ggtctatgtg gcagcggtct ccggtgccat tcacatggcc aactactatc
    16681 gagacacgct gcgaacacac gagcagtggg gattaccgga cccggctgtg ggcgcgccag
    16741 aaaaggcact aaaaagtgct tttttgccga tatttttggc gactttcacc acggcattcg
    16801 gattggcttc attgtctatc agcgagctag tcccgattca gacattcggg attttttctg
    16861 ccgccggcat tattgtgagc tttttgttcc tgtgcctcta cgtcccggcc atgctccaag
    16921 aatggccggt acgcaatatt accaaaccga ggcggacagc attttttgat ccgggacgat
    16981 ggtcgggatg ggaacatgtc ggcgagatca tattgcggcg gggttcctgg gttgttgccg
    17041 ggtgtatcct gcttatggtc ctcggtgcca ttggcgctag ccaaatcaca acctcggtca
    17101 aattaatgcg tcttttctct ccaaaggcca ggatcatcca ggattacgcc tggttagagg
    17161 aaaagttagg accccttgtc ccaatggaag tggtcatcaa agttgatcgg gaccgtgcga
    17221 agctcgattt gcttgaccaa ataaaactca tcagtgaggt tcaacacgcg attcagcaac
    17281 ttcccgatgt gggtagcgtc gttgcagttc ctacgttcgc ccgaccactg ccctctcggc
    17341 caggtttggt agaaaggcgc acgtggatcg tgcaattaca acggcaccgg gaagaccttc
    17401 gcgactattg gtcggaagaa gaaagttgtg aattgtggcg aattagcgct agggtgaagg
    17461 ccctcaccga ccttgactac ggtgtctttg taaacgacat ccgacaggtg gtcgaaccgg
    17521 tgctggccaa gtaccgtgct cgcagtgcgg acggtgtcac ggcaacttac acgggtctcg
    17581 ttcccctggt ctataaggcg caacactcgc tgctaaatgg cctcttgaca ggctttgccg
    17641 gcgacctcgt cctcattgcc gtcgccatgg tgtttgtcat gcgggattgg tcggctggct
    17701 tgctcctggc cctggccagc gtgttcccca ttgccgtggt tttcggggca atgggctggc
    17761 ttggcatcgt ggtggatgtt ggaacggtga tgacacctgc cgtagccctg ggtgtcaccg
    17821 ttgacgacgc tatccacttt atgttgtggt gccgtcacgg tcaggagcgt ggcatgaacc
    17881 gccacgaatc gatcatgttt gcctatcgtg attgtgcaca ggcaatttac caaagttggg
    17941 gggttatcgg tttggggttg tcagcatttg ctctgagttc gttttcgcca acccagcgct
    18001 tcggttacct catgctcgcg atgctaacgg cttcgtcatt tgggaatctt gtcctgctgc
    18061 cagccctgtt agcaagtccg ctggccaatt acttctggcg gagtggcgcg aagcaggccg
    18121 aaagaaaacg gagccagctc aaaacgcaaa aggctcccga cctacatccc cacgttattc
    18181 ctttggagca accagccttc gaggaagaac ccgtgggcgt gtgtgccacc tcggaagggg
    18241 taatttatct cgaaacgggg ggtgacggtg tgacgccgag ttttccatca gagcgtgatt
    18301 gtccccagcg gtggtcgggg gcaaggaagc accggctgcc ccacaagccg cgttgacagc
    18361 gcctcaggct tttgtgcgat tggctccttg gttcgtgcca gtgatttgct gaccataatc
    18421 gtccggcagg ttgttttggg gtaggggtca ctaaaaaacg gcccgtcacc tcttgcctct
    18481 cattgtgaaa cgatttagtt taaggggttg tcgtttcgtt aattcggaga gcatcccccc
    18541 atcttgggtc aagacagggt gacaattggg ttactttcga gatttgcaat aaaggttcct
    18601 tttcatggcg caaaaagcct caactcggga gattccttat ctggcacagc gttctcgtaa
    18661 gggggtcatc accggagtcc aggttttagg gttgggaagc tatttacccg agacacgggt
    18721 tcgcaacgag gatctcgtca agcttggttg tgatccagaa tggattattc agcggacggg
    18781 aatcttggag cgccggcatg cgccgcctga aatggcaacc agtgacatgg ccgttgaggc
    18841 agctaggcga tgtctgctca gtgcccaggt caatcccaag gaagtcgatc tcgttgtctt
    18901 cggaacatac acgcccgata tgacgatgcc ggccacagcg agtctcgtgc aggaaaaact
    18961 gggactcttt gccccagcca tggatatcca ggctgcatgc acgagtttta tgtttgcact
    19021 gatcacagga atgcagtttg tcgggacggg ctgcgccaag cgtcctctgg tcatcggggc
    19081 cgactgcaat tcacgggtct gcaatcccaa cgatcccaag acgtttccca tcttcggtga
    19141 tggggcaggg gccgttgtcc tgggaccggg cgacaaggat caggggttga ttgcgttcag
    19201 ttacgggtcg gacggggccg gatttgatct cctttatcgc cccgcagggg gaacccggac
    19261 ccgaaaagac gagccggggc agctagtcga cgacggctat ttggtcatgg aaggccgacc
    19321 ggtattcaag tgggccgtcc gcatgcttaa tcaaacggtt cacgatgtat tggaaatggc
    19381 ggatcttacg ttggcggaaa tcaagcttgt catcttccac caagccaact tgcgaattat
    19441 taaagccgcc gcggaaacca ttggcattcc cgaggagaaa ctctataaca atctcgatcg
    19501 atatggaaac acatcctcgg ccagcatccc catctgcctc gacgaggccg ttcgagaggg
    19561 ccgtatcgaa cgcggtgacc tcgtcctcct aagcgggttc gggggcggtc tctcttgggc
    19621 caccggcatc ttgcggtggt gatcagcacc ggcgtgcaaa gctctttgat ctagaaaaat
    19681 tgtgccctca cattgtctga ggtccgttcc acttgctcaa ttgagcccgt ttttgccgcg
    19741 tgcgatacca ggtccggaaa atggctgcca catcattcca catattggaa tagtcttggc
    19801 cagaagcagc caagccaata tgatgaaggg ctaaagtcac cccaagggtc acggcaattg
    19861 attgttcctt aacggcctgt accgcatacg gaggtaactt ggcttgaaag cgtggcgact
    19921 cgttgcgctt gtcgctgtaa ttgttgcggg catgacatat gctagcgaaa catccccgcc
    19981 aagtctgccc gcttttaagg tcgaaagccg ggtcgatttg ggtgaggcag aaaaggctgt
    20041 cacttcgagc ataaccatct tttcgcggac aaaagtgttc gattttctag aaaaacccgc
    20101 ggaagtgacc atcatcgatt ggagggacca acgtgtcgtc ctactcaacc ccttgcatca
    20161 tgagcaaacc gaactgagtt ttgacactat ttttcgattt caagagcgcg ttcaagcccg
    20221 tgcactgcag gacaaggacc cacgccaacg gtttttcgcc aatcctgcct tcactatccg
    20281 gatggactct acagaaaagg agttggtctt tgccagtaac tggttaacct atcgagtaaa
    20341 ggtatccgac acagtcgacc cagccctcat acgggaattt ctgcggttct cggatgtgat
    20401 ggctcagctc aattgcctct tggtgaaggg gtctcgcctg cctcatccgc ggataaaggt
    20461 caacaagacc cttctggaac gaaacctctt tccggaagag gtcatcctca tcaacggtcc
    20521 aagaaatttt ttcgaatggc tacccggtcg tcgttcgatc gtgcggagca ctcatcatct
    20581 ggtgttggaa ttaacgcccc aagatcgtgc acggctcacg caagccgaag agtggacgca
    20641 gacattccgg cgcgtcaact tcatcgacta tcaggcgaag ctcgcggcag aataagccgg
    20701 gactcgcggt agcgcttttt ggtggcctaa ccgttcacaa gcgggtcttc tctgcctctt
    20761 cctcgatggc tttgacttcc tccggtgtca attcccgaca tcggatactg cgaatcacgc
    20821 ccgtggagca ccacgaagcg aaaccaaacg gtttgcagag gtcaacttca taacgagtgg
    20881 aaatccgtcg atcgccgata actaaatcaa ccaccttttt gtcatcaatc cagacctcaa
    20941 ttttcacttt tgttacccgc acgcggaact tgtaccactg attgtccttg aacaccataa
    21001 actgggttgt ctcgttatca gaggcatcgt tgaagttaat agatgaaagg ccgataaccg
    21061 acccacccca tccccctgtg acaaagctac agaaggtgtc accgacggga aacgtcaccg
    21121 tggcaaagaa atcgatccca tctgtccttt tggcgacata ctcgacctcg tagttcactt
    21181 tggggaattc accctcccat gcgatacctg tcatgggatc cccgcgctcc atgacgatac
    21241 acccgtcttg gacgcgaacc tcaccctcac cgccgaactt gggtaccttc cagcctttca
    21301 atgacttacc gtcgaaaagg tcccgccact tatagggaga ggttgccgac tctttcttct
    21361 cctcagcgga tttcgtcgga gaagagggct gggcagaaga ttgcgactca cctcgcacca
    21421 gtgcagccca agacccgaca aaaaaggtcg cgcaaagaat gccgatgaaa agtcgtccga
    21481 ggcttaattg acgcccattt gtcattctct cacgaatcat cgtcaacctc cgtcgctctt
    21541 gcgttacact aatcctcctc gggaaactaa tccgtcgcga gcctgttcgg cgactttatt
    21601 atgtcacgac tcacttcttc agtgaagcat gacaagccga tttcagtcag caatcatccc
    21661 gctgtgaatt accctggcct gccttgcgtt gatccaatcc cgcacaaggg ctgttcttgc
    21721 gtattctcca accaggtgag gcgatggtgg gcgtccctcc atactgtagg atcacgcggc
    21781 gccttcttcc ttcaaatctc atacgctttc ggtggtcgaa atcttttgct accaacggaa
    21841 gatacggcgt gaacgctgct ccttttcgtc aaatgtggga aactctatga tgtccagtgg
    21901 aaacatcgtg accacgttct gtacggattg tagaaggatt cggcatgcta atctcgccac
    21961 aaataagcaa gaaggccctg gctcagttat gccgccgtct ggcaacacag acggaagccg
    22021 gcttggaaat ccggacaatt tgccgccgcg aggctcaacg cgccaccggt tggattaatc
    22081 gtcgagtctt ttcccagatc agccaggctg ttgaccacgg ccagagcctt tccgacgcct
    22141 ttgatgcgac caacgatttt ttcccgccgc ttttccggga gattgtgcgg atcggcgaac
    22201 aaagtgggca tcttcccgag gcatttcgcc ttcttgcgca gtattacgaa gaagaattgc
    22261 atcgtcgccg tacattctgg atgcttttgg cctggccctt gtttgagttg tctcttgcgg
    22321 tattggtcat tgggttcctg atttggatta tgggaacgat cagtacccag tcagggatga
    22381 caattgaccc gttgggtttt ggccttattg ggacccgcgg gttagccatt tatgccacct
    22441 ttgtggcagc gtgcatagta acggtcattt tcttgatttg ggctattcga cgcgggatgc
    22501 tttggacgca accggttcaa tatcttgtcg atatgctgcc agtcctgggg gagatttatc
    22561 gaaccctggc cttggcccgt atggcctggg cattgtccat cgtggtgcgc tcatcgatgg
    22621 atgttcgctc tgctgtcgat ttggctttac aggcggccaa ccacctcagg ttcacggcaa
    22681 tccggcctcg tgtctggcgt tcgcttcagg ctgggaagtc gctctatctt acctttgccg
    22741 aagcagaaat atttccccaa gattggttgg acgtgctccg cgtcggtgaa gagagcggca
    22801 aactggatga agctctcagt cacctcagcc gtgtttatgc cgaccgcgca gcccaggcca
    22861 tgaaaatact cagcgtcgtt ggggccgtgg ccgtgacgtt attgatcgca ggcgttatta
    22921 tcttcctaat ctttcgcctt gcgctttttt acattgggca gattcagagt gcattgccag
    22981 cttgaagaac cccagcacac cttgacatat cacattcagg tccgttccgt gacagcgtct
    23041 tggccaacga ggtctttcag cgccttgaga aacgtgtcgt atgtgctttg accaaacagg
    23101 acaaatcgaa ccaaggccgg ttttccatgc tgccgcaaga actccatcgt ggttccgagg
    23161 gcaatcttgg ctgcctcttc aacgggataa ccgtaggccc cacaactaag ggcaggaaag
    23221 gcaacgctct ggcagccatg ttcgatggct agactaagcg atttgcgata ggccgaggca
    23281 agcaattccg cttcgccgtg ttggccatcg cggtaaatcg gccccacagc gtgaataaca
    23341 tactttgccg gcagccgacc cgcgccagat attacggcag aacccgtggg gcaaccctcc
    23401 ggatatttag cacgtgtttc ggccatgatc tcgggtcctc cacggcgatg aatggcacca
    23461 tcgacacctc cgccaccggc caaacggctg ttggcggcat tgacaattgc atcaacctgc
    23521 tgctctgtga tgtccccttg aacaagctcg accacacatc caccaaggga gacgcttacc
    23581 ggcatatctc tgtctccatg caacgattag tccgagaatc cgccttgaaa cctcactatc
    23641 gacggaaagc aaattccccc gaaagatttg actggcaacg cccacaaaaa cgcaacccgt
    23701 gtggcatcaa ctaaaccggc ctaaaaagcc tttttcctgg cacaacccta ttgtgcggac
    23761 gcgcgaaagt cttttcaacg cctcgccctg caagtctcct ttggaaatcc gacaacaata
    23821 aaaggggatc gtcgccgggc cccaatatcg ctcaacgatc gcgccattgg tatgataaat
    23881 agcattatgg gatcggcttg ctccgatcga gtgagccggc ccctcccgaa gtccgatacg
    23941 cgtcttccct gtcatgcgtt tttcgagaaa ggagttagca tcatgagacg tcgcgagttt
    24001 ctggctgcag cgggtgccgg tgttgccctc tcgaccttcc ccttgaggtg gacccacggg
    24061 gcgttcagtg cacccaagaa aatcctctat ttcacgcggt cggcgggttt tgaacattcc
    24121 gtagtcgccc gcaaaggtgg cgagttggcc ttctccgaaa agatcctcat ccaatggggc
    24181 aaggaagtta acgtcgaggt tgtctgcagc aaggatggcg gggtcttcga tggcgatttg
    24241 gatcagtttg acgcctttgc attttacgcc acaggtgatt ggaccaagcc ggttgatcgg
    24301 cctaacgagc cgccagcgac ggaatccggg gtcaagcggc ttctagaagc gattgccgca
    24361 ggcaagggcg tcatcggatt ccacgcagcc aatgatgcct tccatagccg cggcaacgag
    24421 atttccccgt tcatcaagat gattggcggc gaattcgcct ctcacggtcg ccagcaggaa
    24481 gctaccatga gggttgtttc tcccaagttc cccggctgcg agaaactcgg cgactccttc
    24541 cgcatgatgg aagaatggta tgccatgaaa aacctcgcgg atgatctgca cgttatcctg
    24601 gttcaagaga ctgaaggcat gcaggacgcc tgctatcagc gcccacctta tcctgccact
    24661 tgggcgagga tgcacggcaa gggacgggtc ttctacacct cattaggcca tcgcgaagat
    24721 gtttgggaaa gctccatcat gaagcaaatc agcctgggtg gcctggcctg ggtattaggc
    24781 gaagtgaatt tcgaccccgt tcccaacatc aaggaagtga cacctggagc aaaccaactt
    24841 cccaagtgat ctcagttagt tagacgcatt tgagtgtggt agtcggagca gggggcgatg
    24901 cagttgcgcc ccctgttctg tccgatgtgg cggtttttcg gataggaatt gtgacttgga
    24961 gtaaagattg tgagcttcgc agcgctctct cgacgacgat ttggccagat cgtggctggc
    25021 gccgccagtg ttgcggtctt cacgtgtgag cccctccagg ctaagcgaat tcctgaagta
    25081 acgtcgcctc ttgaggcatt tcgcttttgc ctaaatacgg ccacgatccg ggcgcagcgt
    25141 ttgccattgg ctgaagagat cgatttggcc gccaaggtgg gctatcacgg cattgaacct
    25201 tggactcggg aaattgagga gtacatttcc tcgggcggca aactcgccga tcttcgcaag
    25261 agactccaag atcgcgggtt aagtgtggaa agcgccatcg gttttcccac gtggatggtc
    25321 gatgacgaca cccaacgggc caagggcttc gagcagatga agcgcgagat ggattggatc
    25381 gctcaattgg ggggaacacg gattgcggcg ccaccggcag gggcctataa ctcaccgcct
    25441 attgacctcc gtcgtgtggc tgaacgttat gcccaattgc tggatttggg tcactccatg
    25501 ggcgttgtcc ctcaattgga gatttggggt gcatcagcca acttgagtcg cttgagcgag
    25561 gcggcttttg tattggtgga atgcggtcac ccggatgcct ccgccctttt tgatgtattt
    25621 cacatctacc gaagcgggtc gcccgtggct gggctacggg tgttcaacgg tcggaacctc
    25681 cacgtgttcc acatgaacga ttatcctgcc gatccaccac gtgagaagat ccgcgactcc
    25741 gatcgtgtct tcccgggcga cggtgtggcc cccctgtcca cgattctgca aatcctggcc
    25801 gagattggct ttcaaggcac actttctttg gagcttttca accccgaata tgccaaaatg
    25861 acggccgagg aggtggcgca gaccggtctt gcgaagatga aaaatgccgt tcttacggcg
    25921 tttcaaaagg aaagtagctg aactaatcga ttcgcaccgc atagaagggt caccctggcg
    25981 ggaaacaatg cccagccagg gtgacgtatt acactcggcg agtttgaaag gtctcgaatc
    26041 aggtcttaaa tcccgcgacg agttgacgga gggcgtcggc ctgggctccc aactcctcgc
    26101 tgctcgaggc catctcttcg ctacctgctg ccgactgctc ggcaacctgg gcaatgccct
    26161 ggaccgcgtt gcggacttcc tgggccactg ctgcctgttc cgttgtcttt tccgcaattg
    26221 ccgtaacttt ggcagctgtc tctttgatat gatccaggat gtcgcgcagt gattctccga
    26281 gggtatcgct catttgggcc ccctcttcga caagtcgggt cgattcacga atcagttgag
    26341 cgatttcctg ggcggcacgg tttgaacgtt cggccagctt gcggacctca tcggccacca
    26401 cggcaaagcc cattccgtgt tcgcccgcac gtgccgcctc aatcgcggca ttaagagcca
    26461 ataggttggt ctgtccggca atctcggaaa tcacctgaat gatatcgccg atgcgttttg
    26521 aactttcgcg aatggcgttc atggcggaga ttgacttttc gacagccaca ccgccgtgtt
    26581 ccgccgattg gctacttcgg cgggcgagct gatcggcctc gctcgccagt tgacgaatcg
    26641 aatccaccga tcttaccagt tcctcgatcg aggccgtcat ctgctcgacg ctcgccgcct
    26701 gcgtttgagc cccctgggcg actgtctgcg aactttctgc aacgacgcgg gcaccttctg
    26761 taaactgatc ggcactcacg acgacctggt ggatcatctc ctgaagcttt ccgataaaca
    26821 cattaaacca cttggcgagc tcagcaatct cgtcatttcc agtgacagga agacgttttg
    26881 tgagatcacc ctcaccctcg gcaatgtctt tcaacatatc cacnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnnnn nnntagcgtt caaacgcgca ctcacccggc ttcaatttga
    27061 gtgccttctc gatgattgac tggatgaaat aatggccgtc ggcatccttg gcctcccaaa
    27121 tgcattctcc gtcgcgcttt ccgccctggg aaataatata cgtacctctc ttttcacccg
    27181 tcccctgcaa cacataaacg taacccgttt tcccgacggt aatatccatg atgctctgac
    27241 gcagttcggg cacgtcgtcc cgacgaattc ccacatacaa gcaaccgatc acttctttct
    27301 tgtcgttgaa aatcggttcg tacttcgtga tataccagtc attgaccaca accgccttac
    27361 ctacgtacgc ctcccctctg agaaccgtgc tgaccacagg gtttggcgta ccgtcaggat
    27421 ttgttgccgg gataaaggta ccgatggccc ggccaccatc cgcgcctgga acgtttgtgc
    27481 agacgcggag catatcgccc tggctattca ttctttgaaa aacggtgcag gtgagtcccg
    27541 tgagttttcc gacctcatcc acaagaggtg ctttttcctt caaatccttc acttgaccta
    27601 gccacgcatc acccaaatac attttcccca attcaacatt ttgcacctgt cccgtgaatt
    27661 gattctttgc ctgccaagtg accttttcct ctttgatcgt gaaatctcca gaccggccca
    27721 ccatttcctt cgccgcttcc atgctctggt caagcctctt gagtaatgcc ttgtggtaaa
    27781 cttccagcat ttccctcaca ttaagggcaa tgtggcgaca ggtgtccttt ccaagggcat
    27841 caatctccgt tcgtacctgc tcacccaatc ttgacttatt gaccgcaacc aacccggttg
    27901 tcacggttgc cgtgagcaaa acaccaatca cagaaaatcc gacaaccttt gtttgtagct
    27961 tcatggtctt tcgtgctctc tcaaaaaacg cacattctac tgttttgcta tcaaggcttg
    28021 ttcccccatt tcatgctgtc acaccaaact atcttgcaaa accggttcaa gggttcgaaa
    28081 cgtttggtcc gccccgtttt gggaagtcgg aacacccgct tccctgcgga accatagcgt
    28141 gtgcgcagcg caacacagtc gcagaattac gaaattttca ccaggagcgc tcgccagtaa
    28201 ttccgaaagg tccggatttg tcaaaaagaa cgcccatttt cccccaattt ctctctgtcg
    28261 gatatgtttc ccaatttcgc tcaccgttat ccgcgattcg gtacgataat tcgaataagg
    28321 gagtgtatcg gccgccgaga tcgtttcgcc gtagtgggcc acccttcgtc aggatagaac
    28381 aacccgcgtt tatcccaagg atcaaatgga gaaggcgact tagcacctga tgtttttggg
    28441 gaaaggagtc aattcccaag agatgtgatc caacgccttt gacgaattta agcaaaattc
    28501 gcgagaaccg agttccttat gccgggtgac attcgaaaag ccgcgatcct cctgatgagc
    28561 ttgccgcgag agcaggcggc ccaggtcttg gcacgactcg atccgaaaca ggtcgaggcc
    28621 gtcaccattg aaatcgcccg gctggggaca gtcaccccgg acgagcagga gcaagcccta
    28681 aaagagtttt cccaggccaa cccggcaggc ttgaccggtg gaaaaggcgg cctggaggtc
    28741 gccaaggaac ttattgaaca ggccctcggc aaagccgcta atgcaacgct ggagacgatt
    28801 aagcagtcaa tcgaggccct tccttttgca tttttacaaa aagttgacag ccaaaaccta
    28861 atcacattct taattgacga gcacccgcag acaattgctt tggtactctc tcacctcaat
    28921 cccagtcagg cggccgagat tattcgaggt ctcccccccg aacgccaagt ggccgtggtc
    28981 aagcgcatcg ccatgatggg tcagaccagc cctgatatta tccacgaggt ggaaaaaggn
    29041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agtatatgga ggcgaagcgg
    29161 gcgtgtcccg acgcgctcct cctgttccgc atgggtgact tctacgagct gttttatgaa
    29221 gacgcgaaga ttgctgccca gaccctcaac cttacgctca ccagtcgcga caaaagtgac
    29281 aacgccgcgc ctatggcggg gtttccgcat catcaactgg attcctacct cgctaaattg
    29341 ctggcggcgg ggtttcgcgt cgccgtttgc gatcaggtag aagacccgag aacggccaag
    29401 ggtctagtcc ggcgcgagat cacgcggatt gttacggcag gaactgtcac cgaagagggg
    29461 ctgctcgacc ccaaggaacc gaactatctc gctgccgttg tcccgcaaga cccggcaggg
    29521 attgcctgga tcgacctttc cacggggcgg ttctatgccg ggatattccc cgctagtcgg
    29581 ctcgctgatg aactggtccg tctgcgaccg gccgaagtcc tttggccgga ggatgctacg
    29641 caaagcgtgc ctgccattga cggcatggtt gtcacaaaac ggcctacgtg ggcctttgct
    29701 gccgcccaca cccagcctat cctgcagcga catttcaagg gacaagccct cgaaggcttt
    29761 ggctttgagt cccaagggaa cgatctgccc gccctttgtg ctgctggggc catcttagat
    29821 tatctcaatg agacgcaacg gaccaccctg gcacatatcg aacggttact ccgctaccga
    29881 gtcggagatt ttatggagat cgacgaatcg acctggcgga gtctggaggt gacccgtacg
    29941 atccgggagg ctcggcggga aggatcgctc ctctgggtca ttgatcgaac gatcacgggg
    30001 atgggcggca ggcttttgat cgactggtta accaatccac tactggacca gaaagccatc
    30061 gtcaaacggc aagacgctgt cgctgagttt ctcaatcggc cggagatttg tagcaaggtc
    30121 cgccaggcac tggcgagggt attcgatttg gaacggttac tgggccgcgc gacgacaggt
    30181 cgttgtaccc ccagagacct agcgagcatc gcaagaacct tgcggcagat cccacccctc
    30241 aaagagatac tggccaagtg ctacggtcaa cgcctgggcg aaattgctca gttgcttgat
    30301 ccatgtcccg agctggaagc aaagctgtcg gcggcattgg tggaacaatg tcccctctcc
    30361 gcgacggaag ggggattcat ccgggaggga taccacgcag aactggacga actaagacgt
    30421 ctcgcccagg gcggcaaaca atggatcgcc gcttaccagg ccaaagaaat tgagcggacg
    30481 gggattccct cgctcaaggt gggatacaat aaggtcttcg gctactatat tgaagtcacg
    30541 aatacccacc gggataaggt cccggccgac tattttcgca agcaaaccct caagaacgcc
    30601 gaacgttaca tcacgccgga actgaaggaa tacgaggaaa aggtcctttc cgccgatgag
    30661 cgggccaagc aacttgaata tgaaatcttt gttgaattgc gagagttctt gctccaattt
    30721 cggagtactc ttcaacgaac tgccgcgctg ttggccgaac tcgacgtcct ggcgggctgg
    30781 gccgagcttg cgcgagagcg gaattattgc cgacccgagt tgtgcgagga gcccattctg
    30841 gagatcaccg agggacggca tcctgtgcta gacattaccc aaccggaggg gacgtttgtc
    30901 cccaatgatc tccgttgtgg tcccgagggc tttatcattt tgatcacggg gccaaacatg
    30961 gccggaaaaa gcacttatat tcgtcaaaca gccctaataa ccattcttgc gcaaattggt
    31021 tgctttgtgc cggccaagag tgcacggatc ggaattgttg accgcctttt tgcccgggtt
    31081 ggcgcaagcg acgaactcgc ccggggtcga agtaccttta tggtcgaaat gattgagacg
    31141 gcacggattc tcaattcggc caccaagcgg agtctcgtga tcttggatga gatcggtcgc
    31201 gggaccagta cctacgacgg gatgtcgctg gcctgggcca tcgttgagta tctccataat
    31261 cgcattggat gccggacact tttcgccaca cattatcacg aactgacaga actggccgaa
    31321 gtgttgcccg gggtcaagaa cttcaacgtg gcagtccggg agtggcaaga tcaggtgatc
    31381 ttcttgcaca ccattgtgcc aggagcagca gacaaaagtt acggcatcca tgtggcccaa
    31441 ctggcaggtg tgccccgtgt ggtcatcgag agggcgaggc agattttgga agacctagag
    31501 acaaagcatc tggaaggccc caacccgttc cggcaagtta cctcttcatc atccagcgga
    31561 caaggtcggc ccatccaact gacgcttttt gagcccatcg accatccttt gatggaggag
    31621 attcgcgaac tcgatttgac gaagattacg cccctggaag ccttcgagaa actgcgaaag
    31681 tggcaggagc aattggcatc gcggcctacc aaacgccgcg ggtggcgatc gacttaacca
    31741 tgggcttctc atatctcgcc gcttttttct ttgaacttat cttgcccatt tgtcgcattc
    31801 gcctcacact cacatcttat ttatcgcatt gagccttgga gcttcggtaa gtgtgggttg
    31861 aatgccgaaa acccctacta accggaaaag tcatcctcaa ttgcctgaga agatttgtcg
    31921 attgtatcag taacacgggc aataacggtg gtgttgcgtc tattgttttg gtgaactgtt
    31981 gaggatggat aagacttttg gggatcaagc atgcacgcca ctttgaaagg ccaggatctc
    32041 gccaaactgg tatggaacga attaattcgt gttgtgggac tcagcttgac ctggacgacc
    32101 ctctttgcga tcgccatggg ggctgacgat cgcacattta aggcgttcag tctccagcac
    32161 gcatcccctg ggcaggtcga gtcagtcttg cgttctttag tgtcacccgg cacagagata
    32221 attcaagaca ccgcagggcg tcgcatactc atttctgggc ccgagaccga tctccaaatc
    32281 gcagccagtg ttgtcgcggc actggatagg ccccaggaga cttcgccgca gccgccggtg
    32341 gcaaaaccgg ctgtttccca gataaagagt tattttgtct ctcgtgaccg tttgcagtcg
    32401 atgctggcga tccttcaggc acagttcgcc aaccggtcgg atgttcggat cacagccgat
    32461 gagcgaacaa gccagatcct tgttttggga cctcaagatg ttcatgcatt cgtaggtggt
    32521 ctattggcac aggggcccac ccctagccaa acgggtacca cttcgcaggg acgaacaccc
    32581 gtgtcaagtt ccggttcgcc ggcgaatcta cccgcgggac aggcccagca gtcaggtacc
    32641 ggaacaagtt ctagcggacg cactttcccg gcgcaaatga cttacgcttc agcagagatg
    32701 aaccgcgaca catctcagat gggcagttcg ctcacaattg aagagatacc gctttggaac
    32761 ctcaaggcaa ctcagctcga agcggccttg cagtcaatgc taggctccac cctcgaaccg
    32821 atcaatgttg tcccccaagc cgcacgggcg gaataccaaa tggctcttca cactggggaa
    32881 aagctaggtc ttctagtgga tcgtataaac catcgtattc gggtgaccgg ccccatgtgg
    32941 tcactgatgc cagcccgtca gctcattcag gctctagata gcccggcctc aagcgcaggg
    33001 cagaccgtga ggctcattcc tttggccaac gcgcgttcca gcactgtcca tcaggcagcg
    33061 caggctattc aggcggctaa tagcacacaa caggaagcgg aaaaccgttt gcctcaggac
    33121 ctcgttgtca ctcaggttga accaagtcag ccaccgacgc cacaaccgtc agttactacc
    33181 acccaagaac agccacaagg ccagcagccg tctgctccgc aaccgggcgt cacccagcca
    33241 cctgcggtgc aagcccctcc ggccgaacct cccgcccccg gggaagccgc ccctggaggt
    33301 gccgcacccg aggaaggtgg tggacttgtg ggcccggttc agattgagtt tctggagggc
    33361 ctggacgtcc ttgtggttcg tgggaatcgg cgggacgtgg aacgggtcat gcagattatt
    33421 cgtcagatcg aagaaatcag cgtccagacc gagcctgaga tccgcatcgc cttgctgcgt
    33481 cacgtcgatt gtgaagacct actcgcgatc ctcacacagg tctatcaaag cgtttatgcg
    33541 gcacggcgag gcagcatcac gatgattgcc ctcgtgaaac ccaacgctat cttgcttgtt
    33601 ggtcgtcagg aagccgtaaa caccgtgctt gagctgatcg ccaagctcga tcgtcccgtg
    33661 gcaccggaaa gtgaattcaa agttttccca ttgcgccatg cgtcggcaac cacaatcgaa
    33721 cagcgattga ccacttttta tgctaaccgg ggtggattgg gaacaaagat tttaattacc
    33781 tcagattttc gctcgaattc tgtcgtcgtg aaggcatctc cccgtgacat gctcgagatc
    33841 gagcagttga tcgccaagct cgatacccca acagccgagg cggtcaatga gattaaggtc
    33901 ttcacgttga agaactctct agccagcgaa ttggctccca tcctacaaca agccatttcg
    33961 gctcaggcag ccggtgcact caggcaaacg gcgacggggc agttaaccgt ccaggcgggc
    34021 gcgcaagccc agcaggagat ccgttctacc cggttacgct ttgtcaccgt ggacaccgaa
    34081 ggcaaacaat tgctcgagtc gggaattatg accgacgtgc ggatcaccgc cgacactcgg
    34141 gccaacgccc ttgtcgtggc tgccccggca gaaagcatgc ccctaattga ggccctgatt
    34201 acggaacttg accgtttgcc ttccgccgaa gcgcaaatca aggtcttcac catcgtcaat
    34261 agtgacgccc aggccttggc agacatgctg caaaccctct ttgggcaggc tgttacggcc
    34321 gcagccggtg ccgccgccca accggcctac cgcacaggcg cccaggaggg agaaagctcc
    34381 ctgatccccc ttcgattcgc tgttgatgtc cgcacgaaca gcatcatcgc tagcggttcc
    34441 gccggcgatt tggcggtagt cgaagccatc ctccttcgcc tggacgaatc agaagtaatt
    34501 aagcggaaga cacaagtctt tcggctaaaa aatgccccgg caaacgatgt agccaatgcc
    34561 atcaactctt acttgacgaa tgagcggcaa gtccgccaat tgaacgtgaa cgtctttagt
    34621 acctttgaat tcctcagcaa ggaagtagtc attgttccgg aaaccgtgag caatagccta
    34681 attgtgagtg ccaccccaga gtacttcgac gagattgtgg agatcatcac gcaacttgac
    34741 gaacgtcccc ctatggtctt gatccaagtg ctcattgctg aagttcaact cagcaatacc
    34801 gacgaatttg gaattgaact gggtatccag gatcctatcc tgttcgatcg aagtgtagca
    34861 ggagtccccg gttttctgtt caacagtaca ggcgcgttgg gaaataacac cacggcacca
    34921 ggttccgaca ttgtgggggc ccaagccatc tctaatttcg gcgtaggacg aggcaattcc
    34981 actcttggct tcggtggaat ggtcttgtcg gcctccagcg acgcagtaag tgtcctgatc
    35041 cgtgccctga ggcagaagcg gcgtctcgac gtgctcagcc gcccccaagt catggccctc
    35101 gataatcagg cagcattcat tcaagtcggt caacaggtac ccaccgtcac gggaacatcc
    35161 gtaactcaat atgctcaagt caacagtgtg agctacacca acacggggtt gattcttctg
    35221 gtccgcccgc ggattagccc ggatggccaa gtcgtcatgg aaattaacgc agaaaaatcg
    35281 gatgtcggtc ccgagtccga aggaattccc atctcaattt ccgctgccgg gcagatcatt
    35341 cgatcgccac ggatcaacac gacccgagca cagacgacaa tcagcgcggg agatggtcaa
    35401 accgtcatcc tcggtggctt gatcactaag agccgttcaa tgaccaaccg ccggattcct
    35461 ggcctaagtg acgttccgat tctccgccat ctcttccgat atgacctggg cgatgcgagt
    35521 cgaaccgagt tactgatctt ccttacacca cacgtggttc gaacggacga ggacgtggaa
    35581 cgggcgcggc agatcgaagc agcacgaatg cactggtgcc tggccgacgt cgtggcactc
    35641 cacgaagatc tggcaccttc acccacgtcc cagtcgccga ccccagccgt accacccggc
    35701 ccagcccccc aggggatcca gcctagccaa gaggcgatcc caacgccccc gccgtccctg
    35761 cccaataacc aggggcaatc acaaccccgg tcaacgcccg gcaatgcgaa ttggaacgcc
    35821 gttcagcaac cggtgttctt tgacactccc atggaaagcc ctgtcgacag caagtggcaa
    35881 gccgacgttg gtacatcaca catttccgat cagccagggc gagataatgg ggccgtggtc
    35941 gacgagaaca gtcaactggc ctggccaaca cctgcctatc cttcttctgg aacacggcgc
    36001 taagtcaggg tgaacaaacg aactaaaacc cagaggcgag agacacccgt gaggagcctt
    36061 actatgcgtc gtcccagcag taaacccgca acaatctccc ggcagttgcc acgtatgaat
    36121 tacgttgttg gcatgttatt actgagtggc ctagcgatta ccgcagtcgg gtgcgccaaa
    36181 ttcgacttac gaaggaatat cccctggcaa gagacgtccg acaaacccgt tcgctctgtc
    36241 gtggcttttt ggacggaagc tgtgattgaa cgaacgtccg gcccaccata tcggggtttt
    36301 ggtggtcgcc tgctttttta ccccgcggac atgagccacc cggtcaaggt tcaaggaacg
    36361 gtcgttgtct atgctttcga tgaagacctc ccgccaccag agaacctaaa accagtgcgg
    36421 aagtttgtct tcaccaacga gcagctccaa caagtttact ccgagtcgaa acttggcccc
    36481 tcttataact tgctcatccc ctgggaggag ttctcgccca agtcacgtcg ggtcagctta
    36541 atcgtccgct ttatcccgga gaatggcccg tcggtcgtta gtgaacaagt caaggtcttc
    36601 ttgcccgggt tggatgaatc gatgctggca ggcaactccc gtgcggcaag tgacaggcaa
    36661 ccgtcgccaa attctaactc ggcagaccct tccttggatg ccgcagatgg ctcacgaacg
    36721 gccgtcacac agtggcctat aagtcctaca agctatcagg aatctgtgaa acaggcacag
    36781 cctgaaagta ggatgcaaga taaaggagta tcctcatcct ccggagtgac cacgcccgag
    36841 aagaaacgtc gcatggagac gctcaccttg aacatatcgc cgcggttagg aaacggggta
    36901 ccgtcccgcc tgggccacgg ggatacttct ctctcaaccg gtgggaatca tgaaggggct
    36961 cagtcccaac agaccagccc ggttggccac gcctcgccaa ataccccggg tgcttcagct
    37021 tctgcacaaa cgcaaagcca agcgaacctg agctctctga atatccagac ccctgaacag
    37081 gcgtacctga ccaccgtgtc tgccaaaatt ccccgtagcc ccggggaact tattgcggat
    37141 ttgaaccggc aaccaccagc tcgttttgca ccttatcgac ccctggttcc agaaggagga
    37201 gtctctcaag gactaaccga tcgttcgccg tcggcactgt ccccttaaga atggccgtcc
    37261 gaccttcgag gtcaacttga acgttcgcag cctgaggtgc attcttcaag ccggggatgc
    37321 gggacaagcg tgccgccaat tccgcggcaa tctgctccga gttttcgaca ggcgggttaa
    37381 aggccacccg gacagtggaa cgaagaacat cgcgctgggt ttgactccgt gacccataac
    37441 gggaactcgt ccgcgatcgt gcgtaagtgg aactgctcct cgaccccaga agccctcgac
    37501 tacttaagcc catcaagctg ctactcccta aggtcgttcg cccggtaagg ccggtggtcg
    37561 tccttcctgt aagcccagtc gtcctcccag aactagagcc ttgcagggca ctcaaggggt
    37621 tgactatgtc tgcgctatcc gcccccacga acgaactaga cccgaagaac tgttcggctt
    37681 gattgactga ttgaaaattc aggaactggc cgctggacat cgtcgaaaga ccactggagc
    37741 ctaacgagct actccgactt gtgcctgaaa tccgggtact actttgcccg gtgagactcg
    37801 acccaaaact ctgcgtccca aaaagtccgc tggaatagcc ggtctgtgct tgagccgttt
    37861 ccagggtgcc ccaaagggaa agggccatcc ccaccactac cgccatcaac cgggtacgga
    37921 gactggtcat gcgaaaaccc tcctcacccc catgtgttcg gcgcaaagaa tcaatactct
    37981 aatctctacg aaacaatagc cgcccgaaga cgttatcgac acccccggcc taacggcccg
    38041 caaaatcatc caccactatt ataaacgctc tacaccgtga aacatcccac cgaaatcgta
    38101 ggctcgtggc ggatttctcc ccatatcgag cggaaaccac cctttttctc ccacctatac
    38161 ccccctgcgg agctacggca ccgctaaggc gcccttacta gccgcatgtg gcaatatact
    38221 gcgaatcaat aaatttcttg ccaaaggata acttgactgc caggttcatt accccagttg
    38281 tggttaagag cctatccaaa aagtccccct ctccctaggg gagagggttg gggtgagggc
    38341 aagcgaacgt cggaatcgat tgagaaaacc ggcaggaatt tgagccaccc ccacccccga
    38401 ccccctctcc cgcccagcag atgggcggag attataggac aggctctaaa tcccaggatc
    38461 ggcaactcgc tgcaatcatt cctctttgag cttaataaca gccatctggt gaatgtcaaa
    38521 cccaggtatt tcaaattcga tacgcccaga ttcgtatttc caagttcgga tttccattcc
    38581 ctggggctga agcaatatga actcaggctc cctctgcaca gccacggaaa cccgacacgc
    38641 cggagcaggg gggagctcct ccacccgaag ggcca
//
LOCUS       DAOSUT010000014        38481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_734_length_38481_cov_9.515588, whole genome shotgun sequence.
ACCESSION   DAOSUT010000014 DAOSUT010000000
VERSION     DAOSUT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 38481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38481
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_38481_cov_9.515588"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLD05_02275"
     CDS             complement(<1..661)
                     /locus_tag="PLD05_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOP76285.1"
                     /translation="MVILGISDLHGRIDALAAILDQVDRCDIVALAGDITHFGSPKDV
                     DQIIELCRSHADHVFAVAGNCDSASIDQRLNELGVGLAGRGVVINGVGIHGLSGIPPW
                     RPGMYQFPEDELASYLQVGFQQIAGSSVHLLLTHVPPRQCGVDRVIWGCHVGSRAVQE
                     FLSTAQPDLVLCGHVHEGRGIERQGKTMIVNCGHAASGYYAKIVIPSRDSDNQIQVEL
                     CR"
     gene            936..2081
                     /locus_tag="PLD05_02280"
     CDS             936..2081
                     /locus_tag="PLD05_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOP76286.1"
                     /translation="MKITAIDLYLLSLPLRTPIRFETVPFSTDPCEWSRLETVLVRLW
                     SGGAYGWGEACPGNGPFWSAEWAGAIFYCLRDWLAPRLIGREYATPEELHERVAVFRG
                     WRAAKAALDIAFWDLKSRLNNRPLYAELGSAPRELPLGKSIDRPGHLTTDEFIKDLAN
                     AFESGYSRVTVKVRPGWDLQMLNFVRHEFPTQDIHGDFEGALTLNHMEILYRLDDFNL
                     CMVEQPLPADDLVGHAMVQEALRTPVCLDESITTPAQADMALELHSAKYLNVNPIRVG
                     GLTPAKAIHDAAHQHCVPCYLGLYPGTTISVRAALALASLSNFCYPPDYWDTAEAFVE
                     DVAEPLQTVRGDQNGRLTVSFWSDAGLGVDPAAEVLGKYCLSECHLP"
     gene            2125..2436
                     /locus_tag="PLD05_02285"
     CDS             2125..2436
                     /locus_tag="PLD05_02285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOP76287.1"
                     /translation="MIHVIAEIVTKPGQRDAVLAEFQKIAPAVRQEPGCLEYGAALET
                     AAGLPVQPPVRDNVITVIEKWESLELLHQHLGAAPLQSFLQKTQHMIESLSARVLSPV
                     G"
     gene            complement(2598..4700)
                     /locus_tag="PLD05_02290"
     CDS             complement(2598..4700)
                     /locus_tag="PLD05_02290"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOP76288.1"
                     /translation="MALYEVKKIRNIALCGHGSVGKTTLLDRLLNFTGAVNRPASVDD
                     GTSICDFDEEEKSHRYTIEAKVTFFDYDGLHYHVIDTPGYRDFIGQVIGAFRAVDLAA
                     IVINAQSGVEVNTRRVFQEAEKAGIPRLIIINKLDAENIDFENLLAGIRETFGDRCVP
                     LNVPIGLGADFRGVATTLKAESSVPGAVIDPSSISQSLMESIVVADEEVLSRYFEGQM
                     PTDEEISQLLGRAMIDGSVIPIVCVSAKTGVGLPELLKVFDLCGISPDRIVRRAQKPD
                     SGEWVELTADPSGPLVAQVFKTRVDPFVQKISFLRAYSGTVRTNATVSVVGERKGLKL
                     AQLFHVQASEMIPIEEAGPGAIVAVTKVEELKTGMSIGDLVMPPIEFPTPMTGLAITP
                     KSRGDEQRLSGALAKICQEDPTCRLDRDPQTKELVITGMSELHLQVIRERLKRRDKVE
                     VDVKEPKIPYRETIQAPAEGMYRHKKQTGGRGQFGEVHIRMFPFPRGTKVEEFATKER
                     FPEMKEVHYHEESNFLWIDSIVGGTIPSNFMPAVEKGFLERISRGVIAGYPVQDVCVE
                     VFFGKHHPVDSSEQAFKTAASMVFRNVFQQAKPALLEPIVKIEVTVPADKVGDINSDL
                     SGRRGRVLGMTSAGGGFQTIVAEVPLAEVITYARALSSITGGQGSYTLEFSHYDIVPG
                     NIQKEIIEKAKLEEEEEE"
     gene            complement(4814..5545)
                     /gene="hisA"
                     /locus_tag="PLD05_02295"
     CDS             complement(4814..5545)
                     /gene="hisA"
                     /locus_tag="PLD05_02295"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOP76289.1"
                     /translation="MEVWPAIDIRGGKCVRLCQGDYGREIVFNSDPVAIAHHWVTLGA
                     RRLHIVDLDGAKIGEPVNFDIVQRIFQESGVPCQFGGGVRDETTISRLLDAGASRVVV
                     GTRAVKDPDWFRAVVRKFPRRIVLGLDVRAGRAATDGWLEISNRTAAEIVAEFADEPI
                     AAVVFTDISADGMLSGVSLEAFRHMQQVITVPLIASGGVTTLEDIRALKEIGVTGCII
                     GRALYEGRLNLPEVLAIAEDKGSCQ"
     gene            complement(6028..6975)
                     /locus_tag="PLD05_02300"
     CDS             complement(6028..6975)
                     /locus_tag="PLD05_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76290.1"
                     /translation="MVAAGGVTTAGSAQFDPLSSVKDGLEKVGELLTFTEPVQQAPDP
                     TALSTKAKPSPRLYVAMARFAEESRQPAMAEQYYEKALSMDAKYLDALLGLGRLKDRQ
                     GQLEEALTCYQKATTAHPGEPTAWNHLGLCYARMNRLDHAKTAFEKAISLSPKESRYR
                     HNLAAVLVQMGDLQGALEQLQAVHDEATAYYNLGYLLVRKGDHAAASRYFAHAVQVKP
                     DFQEARQWLDALSQRSADASKQSQMPPNVELPTPVERPALPDQNLPVPPSGPAGTSPR
                     AFQSAHQVAPLPPVLDENTLEVSDWPSLSAPRPLPPVVE"
     gene            7523..8095
                     /locus_tag="PLD05_02305"
     CDS             7523..8095
                     /locus_tag="PLD05_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76291.1"
                     /translation="MSRSFALLVCSVTLWAYTAYSDSVFAQGVKGFFHSIKVDAVRNA
                     CWPDAFVPADRTNARLPFVIMVHNGWKRQNLIADHYFAEDGKTLTDAGKRQVQWIVTQ
                     APLSHRAIYVKEGEDATITEARVKAVGEVASKYLGPEQKPDIRVTNISPPGWPAELVD
                     TVERAYLKTMPDPRLPARSSNSEAQVELSN"
     gene            complement(8192..8968)
                     /locus_tag="PLD05_02310"
     CDS             complement(8192..8968)
                     /locus_tag="PLD05_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76292.1"
                     /translation="MLQTLGIVDACFASDKERRRAAGRLQGKSILEWVTRRATDCQQL
                     DGVIVVTSSDPANLFVKDLVPLDVPVFVGEGDDHLTIWAKALEAYPCREFVRFETACP
                     FVDQLLVDRLVKEAHRLDEPADYVGYRSHNGTPAMLTSVPVYGELVLTKTLHRLCERL
                     RNPSDRAHPTRYIYSHPERFKIHWLPIPPGLDRDDIRLVLVDEDDWEVHQIIVDTLGP
                     ECDGHHIAHLIERCPSLRKQMEALNEKKSRQAIVKARPSV"
     gene            complement(9149..>9343)
                     /locus_tag="PLD05_02315"
     CDS             complement(9149..>9343)
                     /locus_tag="PLD05_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76293.1"
                     /translation="APWRKPQQKATPESKKPRPSIQRHTTKQAAPARRPEDDPEAFRE
                     KLEEWMRTQMAPKQSGKSNK"
     assembly_gap    9344..9443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9444..10282)
                     /locus_tag="PLD05_02320"
     CDS             complement(<9444..10282)
                     /locus_tag="PLD05_02320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76294.1"
                     /translation="MASEAQIQEWIHRLAAAGLSCDLPKMSEEVSQITSFAQFLEYLV
                     RQKVLTAWQAEELVKGRQSFLLGKYRLLTELGRGGMAHVFLAEHVTMERRVAIKLISS
                     NLPPSARDRFLAEVRTIASLDHPNIVHAYNVDCDGAQYYLVMEYVEGKTLEHMVRESG
                     PLPWRQATDIARQTALGLGYAHAQGVIHCDLKPANIIVNSEGVVKILDLGLARLIKRD
                     RGTQVSQEDPSQVLGTVDYMAPELALTPDQADHRVDIYSLGCVLYFALVGTPPFPDGT
                     LAER"
     gene            complement(10305..10979)
                     /locus_tag="PLD05_02325"
     CDS             complement(10305..10979)
                     /locus_tag="PLD05_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOP76295.1"
                     /translation="MTPSSSRTGVILLGPTGAGKTPLGESLSAWGHGRWRFHHFDFGH
                     WLRIVISDQESQCLFDDDERHLVADILASGRLLKDEEFFIADRLLRSFLSAIPYNSDR
                     MEVIILNGLPRTLFQAERIIRLLPIQLVVNLQCTLDVVFERIHSNVGGDRTGRDDDLE
                     SLVAKKYCLYESETRPLVDYYRGLGIPVVSLAVTTTTTAEELRDQFLDRWSKLFEGTR
                     SKTSMC"
     gene            11370..12548
                     /locus_tag="PLD05_02330"
     CDS             11370..12548
                     /locus_tag="PLD05_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP76296.1"
                     /translation="MKFKIAFAITELEMGGAEKALTELVLRIDRARFEPCVFVLSVPP
                     LDQILVKRLTSHNVPTYFLGLQHWHQLIPVIVRFARMLHQEKVQILQSFLFHANIVSR
                     IAARFAGVPVVISGLRVAEHAARWHVLVDCMTSGCVDHYVCVSQSVAQFAVRNGLPPE
                     KIRVIPNAVDPPEFCQESPQGTYGADKFLPEHIGRNEQNTTAPSCGRTAAGSEYRMVT
                     VARLEYQKGVDWLILALARWLRSYPDLRLVIVGDGPMKSTLQALIHARGLSEQVTLVG
                     FQRDVARILCDADLFVLPSRWEGMPNALLEAMVCGLPVVATDVEGVREILGPCIAHQL
                     VPFGDSDTLTHAILWHYLNRDCSQTIGQQNRTYVLSSFSWPRVVEQYEKLWLWLLQRG
                     "
     gene            13719..15086
                     /locus_tag="PLD05_02335"
     CDS             13719..15086
                     /locus_tag="PLD05_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76297.1"
                     /translation="MSLQTNCLVAFSVCVLVHLAGTVATPSEVAKPNVIVIMADDLGY
                     ETIGANGGTSYQTPHLDRLAATGVRFEHCYVQPLCTPTRVQLMTGQSNARNYIRFGVI
                     DPSVTTFAHLFERAGYATAIVGKWQLGREVDLPRRMGFQEYYLWQHTRRPPRYANPGL
                     EIMGVEKDFQNGEYGPDLLNDFACDFVKRNKDKPFLLYYPLTLTHAPYQPAPDSPDWD
                     PNAQGEQVNQHPRHFGEMVQYMDKLIGKLVDLLDELNIRKHTLLIFLGDNGTGGGIRS
                     MMGDREVIGGKGKTTRAGMRVPLIVNGPGMVRSGVVCSDLVDSTDIFPTICEAAGIPI
                     PDGLVLDGKSFWPQLCGQPATPRKWIYCWYSPRQGADTRVTEFAFNQRYKLYRDGRFY
                     DCELDPEEKQPLEDSQLSEDTRQIREMLKQVLDAHAGIRPAHLDEQLPPPDRPATRQR
                     RARQQ"
     gene            15230..16396
                     /locus_tag="PLD05_02340"
     CDS             15230..16396
                     /locus_tag="PLD05_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP76298.1"
                     /translation="MARYLLLGFVALSTLTVRIPAAELQFELHRIGSFRSEACGVADF
                     NGDGKLDVIAGPFVYIAPDWKAYKIREIKGEVDQAGKGYMWNFADLPLDVDGDDRVDV
                     ISCDWFEKCLDWHRNVGFGGDLWPRSVIDVSLNHETAQLVDIDGDGQKREILPEVVET
                     YWFEIVRDPTGGVRFEKHIISQKKMNFGGGVGDINGDGRPDVIRPDAWFEAPADPRSG
                     QWVEHPIALGGREEGQSEHTAQILVHDVNGDGLNDLIASSAHRYGIYWYEQVREDGTI
                     RFRRHLIDNTWSQAHSLTLADLDGDGDLDLVTGKRFMAHNGSDPGEFDPLGVYWYEFE
                     AKPQPKWIKHAITYDQGIGAGLNIPVVDLDGDGDLDIVVTGKWGGPVWFENKLK"
     gene            16540..17856
                     /locus_tag="PLD05_02345"
     CDS             16540..17856
                     /locus_tag="PLD05_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76299.1"
                     /translation="MDRQRISTRRHFLKVTAGLSAGALMPMIVPRHVLGTETTPSANE
                     RIGIGGIGIGRRGLQLFEEIPSLGRVVAIADVNLPRAEKTAEPYRAVAYQDYRKLLER
                     KDVDAIITATPDHWRAIVCIHACQAGKDIYAEKPMSLTIYEGRKMVEAVRKYGRVFQT
                     GSQQRSMAANRLGCELVRNGRIGKVHTVIAANYPSPWECALPGQPVPAGLDWDMWCGP
                     TEPVPYHIDLYTPRANPGWISFRPYSGGEMTGWGAHGLDQIQWALGTDDTGPVEIWTE
                     GPKFSPPTYTQPESRARGDKICSQPKVFFKYASGVVVKFDNGPPGGAIFIGDKGRITV
                     DRAKFTVEPKELGDPPYDDFSVRLYVSNNHMLNWLECIKTRQKPVADVEIGHRSTTLC
                     HLGNIARWVGRPLKWDPVKEQFIGDDEANSYLDRPRRKPYVLPDPV"
     gene            complement(17891..18265)
                     /locus_tag="PLD05_02350"
     CDS             complement(17891..18265)
                     /locus_tag="PLD05_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76300.1"
                     /translation="MKSRRVQSPQETLKSLRLRRHGLAILTLVLAAGIVFFELRPPSS
                     SLEIMCQAACWRMAPLTALIWLAYYHILALPTWLALVIPAALAVAVFRPRALLLLLPI
                     IIVIAILRPRKRPTNGSRKKAR"
     gene            complement(18401..20428)
                     /locus_tag="PLD05_02355"
     CDS             complement(18401..20428)
                     /locus_tag="PLD05_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76301.1"
                     /translation="MDRDLLRRGKSLSRQRHAGRMFAATLSLALFLTIVSSQVALSNG
                     ADVLRFDLPELPSPEIASIANRVYLVLEKQAHFLLDQLKPWNGDEKYKLLTESRSQEH
                     WIRPNTGAVQAFCFLYRFGPYDETVVGMSREKLLHEAIIPMIRYLTDTHVTGNRPTGD
                     GKPWGDAWQSAHWAQMLGRGAWWIFDSLPEDLQIAVRRVVRHEAERIARSDPPSQLRG
                     DTKAEENAWNAQILSVAMLLMPNDERAPEWDRQFQRWTMSAFLRPADETCQEIVDGRP
                     VAEQFTGANIYDDFTLENHGFVHPDYMGTFTLSLGCVTDFRMTGRKEPASVYWNTAGI
                     YENLKWFLLPDMGYVYPSGQDWTIFRQPAWITCHNVMFVFGRDPEAWALLNQTLDVTE
                     RMQRRSSVGAIYDPSQFFFPSTQTDIAAALAKVWLNLHFADPQSMGQYRPRLGVRHLE
                     FGKLVLHRTPDFVCALSYGPIIMGTITMNRPDRLTSPDQRSLLGHVILDGQTAALPIK
                     LQSVEVTTHPDGFGAKLTVHHGDAVAAEWTMRSHASGTVEIAEKLVAIKDCVTAEVAT
                     GLIGVLNNVNWIYETGQRSLSLNGETWQIPAHSGEKIDREGITQVLVDNALRIEISPH
                     RRVVYRAATKPERGRATDRLYLNGILGHRAFRIGETIAEWSAVLSVIRQAP"
     gene            complement(20540..21532)
                     /locus_tag="PLD05_02360"
     CDS             complement(20540..21532)
                     /locus_tag="PLD05_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HOP76302.1"
                     /translation="MKAAYIDHPGPPEVICYDDLPVPQLKETEVLVRVGAVSVNPIDT
                     YIRAGTVKMPLPKPYILGCDVAGTVEAVGARVTRFKPGDRVWGSNQGLLGRQGTFAEY
                     AAVDECWLYPTPATVSDREAAAIALVGITAYLGLFRDANLRPNETVFVHGGTGGVGSC
                     VVQMAKAVGARVITTAGNEEKADLCRQLGADVVINYKQENEAEAIRRFAPEGVNVWFE
                     TLREQNLEMAVSLLAMRGRLVLIAGRQSKPKFPVGSFYVKDCKAFGFAMFNAPPEEQQ
                     RAAEQINQWLKNGKLRPVIGRVLRLSQAVEAHRLQEQNTLGGAGNLVGKIVLEP"
     gene            complement(21582..22079)
                     /locus_tag="PLD05_02365"
     CDS             complement(21582..22079)
                     /locus_tag="PLD05_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOP76303.1"
                     /translation="MNQPDKLEQLRQQLLDSYKNRALIYYHIYDELARILGPDQAEEI
                     LKRAIYRRGQEREREYARFAPNNLAAVKETFLAHLPDDGRLFEPEILTDTPDALDIKF
                     HRCPLREAWVEAGLPDELVARLCRIAAEVDYGLFREAGFGFFADTWQPGGEGCCCLHI
                     RPGGA"
     gene            22287..23057
                     /locus_tag="PLD05_02370"
     CDS             22287..23057
                     /locus_tag="PLD05_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76304.1"
                     /translation="MASIDSALSAELLPMPSPEIVAQLRRNISTIHRSYRPRQGQKFP
                     IDIPEVMDLFPGGQLPYGSIMEWFTETHGTEAESLSLRIGWSACLRNRFLVIVDPCFE
                     IYPPGLSQLGIDLNRVVFVHPATKSDMLWTIDQALRCSAVGAVWSYLESLDPINHRRY
                     QLASECGGTVGIFVRPLRARKESSWAEFRLLVQPVAFPAGEIGSQCRRIQVTLLRAPG
                     LSEPIVREVWIDDPADRLLDIQRVEPFQRRYQYQKNRA"
     gene            22975..24402
                     /locus_tag="PLD05_02375"
     CDS             22975..24402
                     /locus_tag="PLD05_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOP76305.1"
                     /translation="MTQRIACLTFSGWNLSNAATSTKKTGHNHASSPGLQEDDVRKRQ
                     DRLAEICLRYTPHVGLVEDPHSDSLFLELTDIQHLIPDERRFVIQLLMDCHREGYFPT
                     KIAVADTPGAAWAMTHFGTGPRATEISSAWILPPSESKKWIASLPVEALRIPDLVAEW
                     CHELGLHRIGDLIHFPMAELRKRFGEILVLRLEQLLGSRQEILSPYLPQPQFEEHLWL
                     EYPARSWEHLSPSLSAFLERLTQQLISHNVGALGLRVNLLCEDHSTVTLSVSLFRPSI
                     DPHHLSGLIELGFRQKHFSSPIVGINAVVSHVAPLESHQATLFPDGETDWPSSEWGHL
                     LERLSCRLGTARVCYAVLNPDHDPEKAYFWYPCLSHRKSHKPRSDGKALVTKGSRPVR
                     LFRAPIPIQVVTAIPSGVPYQIHHQGQSHHVVRWWGPERIQTGWWRGESITRDYYRVE
                     LQNGVHYWIYRCLESGTWFLHGTFG"
     gene            24471..27794
                     /locus_tag="PLD05_02380"
     CDS             24471..27794
                     /locus_tag="PLD05_02380"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOP76306.1"
                     /translation="MYCELHVKTNFSFLQGASHPDELVRQAAELGLAGIAITDKASVA
                     GVVRAHVTAKELGVKLIIGAEIVPKDSLPVVLWATDRASYGRLCRLITKGRCRSEKGE
                     YLLTFDDIAEHSQGLLAGVIPRVRDGQTILHHLQQYRGVFGQRAYLLGEILLGPNDRR
                     QLEWLQHLSHRSRLPLVAAGNVFYHHPSRAALCDILTAIRLGTTVDALGEHRFANAQR
                     YLKSFEEINALWYRCPEAVARTREIADLCRFSLDDLRYEYPQELSPPGETPFGYLQRL
                     VWEGAKKRYPKGIPEKVSRLLEHELKLIHELSYEPYFLTVWDLVRFARSRGILCQGRG
                     SAANSAVCYCLGITAVDPERMDLLFERFVSRERNEAPDIDVDFEHQRREEIIQYIYQK
                     YGRERAGMTAEVITYRPRSAVRDVGKALGFSLQEVDRIAKQIERFHEEPDLLERCREA
                     GIDVDQLATQHLVQWATTITGFPRHLSQHTGGMVITQGPLCELVPIENGAMPDRTVIQ
                     WNKDDLDELGILKVDCLCLGMLTAIRRTFDTVFKHYGRKLSLASIPEGDPGVYAMIRR
                     ADTMGVFQIESRAQMNMLPRLQPRCFYDLVIEVAIVRPGPIQGNMVHPYLRRRNGEEP
                     VTYPNEVIRQVLGKTLGVPLFQEQAMRLAIVAAGFTPGEADQLRRAMGAWRRPGLIDA
                     FRRKLINGMLANGFTPEYAEAIFRQIRGFGDYGFPESHAASFALLVYVSAWLKYHYQA
                     AFTVGLLNSQPMGFYAPAQLITDAKRHGVEIRPVDINYSYWETDLEGATAFPVVSSER
                     DSSWGRCPENVESLSEDGGSLFPFSEDENSQWHSSPVPDDILRSGSKHSFPESSLLPA
                     KAIRLGFHMVHGLRESDVEKIIEARKDGPFTSIADVMRRTGLSQATIIRLARADAFGS
                     LGLRRREALWEARALSTATPPPLFQDRTSSDQSLAGGISSSADTREQSHSPPSMNLAE
                     EVLADYAMIGLSLKAHPLRLLRKELNQLQVVPAERLLEIPDGHPVRVAGMVLVRQRPA
                     TAHGITFMTIEDETGIVNLVVRPDVWQRWRSAALNATLLFAVGRLQKQNGVIHVVAIR
                     LEDLSAKLAGLQARSRDFC"
     gene            complement(27902..29833)
                     /locus_tag="PLD05_02385"
     CDS             complement(27902..29833)
                     /locus_tag="PLD05_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP76307.1"
                     /translation="MTRILAAIFCLITIIAGRLCVAEDVSTLTDSAKLVAILESKESS
                     DFEKAVACKRLAVFGTAEAVPVLAKMLLDEKFSHYARYALEPIPAAVVDDALREALSR
                     AEGGVRLGILNSIGVRRDTKAVVAIAPLLNHDEPAVVDAAASALGRIANVEAADILKK
                     SLQSEKATTRRAVGDALLICAEIVKKEGNTELAIDIFDAVRKADVPKPVRLAAMRGAV
                     MARGDNGVTLLKELLETDDSGVFDLALRVAREMKSAAATEELVKHLGVVSEDRQAKIV
                     EALADAGVQEALPAIRKAVAEASGAMKLAAIRSLAKCGDKSDVALLLEVAKSSDEVVA
                     EQAKAALVSLQGPGVGEALADSLAKEDSKGKLVLLEVVGQRRTLAASPVVRQLAASAD
                     PSIRLAAIRTLGNLGQDEDVAMLVERLITSPSADDVAAVKDALQAASQRVRDRDAIAK
                     MLIQHMDKAPGNTRRDLLEVLGSLGGPTALAAVAGAAQSQDSSLQDAATAILGNWLSP
                     DVAPVLWDLIQKNAAPQYRIRLIRGYIRVARQFNVPDAERLAMCRNALQVSDRPDERR
                     LVMDVLRRNPSAEGLALAVAEIDRPGMRAAACETIVTIAEKLQKNVPGLREALSQVVQ
                     KATDPAIKARAQAILERLP"
     gene            complement(29881..31155)
                     /locus_tag="PLD05_02390"
     CDS             complement(29881..31155)
                     /locus_tag="PLD05_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76308.1"
                     /translation="MRHVTKICRRRFLGTSIGALSALAVPSLIPGKALGLNGFVAPSE
                     RIVMGGLGIGPRGRTDLAAFLKNPDVQFVAVADAQAARREAVKQMVDRHHQNSDCKTY
                     RNMFDLLLRDDIDAVLIATGDRWHTMASIYAAKAGKDIYCEKPCSMTIAESRALAETI
                     RRYGRVYQAGTQRRNIGNFIFAINLVYSGKLGKLHTVHANTLDPRTTHEWLPAEPEPP
                     KDECDWDLWLGPCPWRPFNQRYVNGGWRGHFDFHGGGILEWGSHTVDLCQWANKADNT
                     APIEFEPQGNHVHATYANGVKLIMRDTGWLGLGTCSVRFEGDEGWIETGDSGQVAVYP
                     ESLRSEMPVFTMAGTDPSTHIRDFLNCVKSRAMPAANADVAAQSHIACHAAYIAWQLG
                     RKLRFDPVKEEFIGDDEANRMRSRALREPWHI"
     gene            complement(31213..32262)
                     /locus_tag="PLD05_02395"
     CDS             complement(31213..32262)
                     /locus_tag="PLD05_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP76309.1"
                     /translation="MRVHQMASFRGVFGLAIALLLASGWSFYAFADEDGPWVVYRGQQ
                     GPGLGKHIVLISGDEEYRSEEALPMLGKILAKWHGFTCTVLFAIDPATGEIAPNVRDN
                     IPGLEALENADLMIIATRFRDLPDEQMSHIVNYLKKGRPVMGFRTATHAFAIPAGKTY
                     AHMSWDCKEPGWEGGFGRIVLGETWIAHHGAHNKESTRGVIAPEMKDHPIVRGCDDIW
                     GPTDVYRVRLPLPGDSQPLVLGAVLEGMSPQDKPVEGPKNDPMMPVAWIKTYTWENGP
                     AGRVFTTTMGAAVDFKSEGLRRLVVNAVYWCLGMEDLIPKKANVEIVGQFEPNYFGFN
                     GYKKGLRPSDFAGLD"
     gene            complement(32396..33643)
                     /locus_tag="PLD05_02400"
     CDS             complement(32396..33643)
                     /locus_tag="PLD05_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76310.1"
                     /translation="METIRYGIVGGGFYAKFQLRALEQVRGIEVAGLVSRRPPEALSQ
                     FVQAKRLGEGRIFPSVREMAHHVDVVAIYAPNFARVPIVEEIGKALDEGAKLRAVICD
                     KPLARNLREARQMIDRLRPFGVPLIYFENQLHMKAVQQARAQLEPVVKTMGPLTLVRA
                     SEEHAGPHSAWFWDPVQQGGGVMTDMGCHSVAVAWYLLTPPGKPLGFLRPIAVQADLS
                     LLKWGRPQYREALRQRFGVDYSKTPADDFATGLITFLNPETNCISKAQFTVSWMYDKQ
                     GLRVMIDGIGPGYAFEFNTLRSPLEVFVGDVAAAAVADAEAALEKQQATRGLVTVQPN
                     EPDLYGYVDENIDALESLLTGRPALMDWDFGREIVKLTMAAYLSAERRATVDLTDPQT
                     LAYLEDYVPLIQQGRGREVLHIP"
     gene            complement(34241..34414)
                     /locus_tag="PLD05_02405"
     CDS             complement(34241..34414)
                     /locus_tag="PLD05_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76311.1"
                     /translation="MRIRRPLSLAAIGLATVLATANSVFAVLPREDERADARTWVGQY
                     LKDGGGVPFFVPL"
     gene            complement(34513..35148)
                     /locus_tag="PLD05_02410"
     CDS             complement(34513..35148)
                     /locus_tag="PLD05_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76312.1"
                     /translation="MRSCKYLILLAVVGLTSVASLVLAAQPPSGQWKPLFNGKTFQGW
                     HIIGVGQWTIEDGIIVGRKNAAEKEFGHLVSDGAFGDFVVRLKFKALQGNSGFYFRCV
                     EEGHSGISGFQAEIAPDANTGGLYETNGRAWVVRPDPELVKKAFKFQEWNEMVVSAQG
                     GDITVWLNGIKMSEVKNDPGRREGHFALQLHGGNDMLVMFKDIEILEQKKP"
     gene            complement(35567..36505)
                     /gene="rbsK"
                     /locus_tag="PLD05_02415"
     CDS             complement(35567..36505)
                     /gene="rbsK"
                     /locus_tag="PLD05_02415"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOP76313.1"
                     /translation="MNDARVIVLGSINTDLVVKVPELPRPGSTVIGGTFYQSQGGKGA
                     NQAVAAARVGRSVVTLIAAVGDDGFGRESLAHLGQEKIDLEYVVTKPQSPSGVALIIV
                     DQHAENMIAVASGANALLSPSDVAAVPETVFRSAKVFLTCLEIPLETVIAGLKRAKEA
                     GLITILNPAPALDSPPPEMLKSVDIITPNQSEVSRLAGVEVSDIDSAQRAAYVLRQKG
                     VNKVIVTLRSQGCLVVDAERTAAYPAFRVNAVDTTGAGDAFNGALAAALAENQPWEEA
                     IRWATCAAAISVTRPGAQTSFPTRDEVEQMLTKAGS"
     assembly_gap    36726..36825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36826..37804)
                     /locus_tag="PLD05_02420"
     CDS             complement(<36826..37804)
                     /locus_tag="PLD05_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOP76314.1"
                     /translation="MIRDPEETIAAIATPKGFGARLIIRISGPETWQAVHRVITICDN
                     DLPVVPAEPVIRAASLRLRQLHGTVPCELYYWPPGRSYTGQAVAEIHMWCCHPLAEAV
                     LAELQVRPAEPGEFTLRAFLSGRLDLTQAEAVLGVIDAVDPEELRHALDQLAGGLGTP
                     LQKLREGLLDLLVELELGFDFVEEDLPFLSRDVLWQRLSQARQSVTEIIERAFKRRTA
                     DELPMVVLLGRPNVGKSTLFNALLGGTRAIVSPVPGTTRDYLTAVCEHGGVKFQLVDT
                     AGLWQLVQSVSSQMPMGVESTENAARYHSFQGSNSIVDQAAQAKARALIE"
     gene            complement(37814..>38481)
                     /locus_tag="PLD05_02425"
     CDS             complement(37814..>38481)
                     /locus_tag="PLD05_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOP76315.1"
                     /translation="KDLQERNRATQELFRQHNYNPFSGCLVLFIQLPIFIGLYKALAI
                     DVELRQAPLISESIRWCSNLAAPDMLFDWSFLWPEWFNRGQGMFALGPYFNILPILTI
                     AIFLWQQKKMMPQPTDEQAAMQQKIMNFMMIFMGILFYKVPSGLCIYFIASSLWGMAE
                     RQILLKTTQKRPDRGAEQRKQRKQWSRVKIRLPQWLVQLLKAADKNQAEKLARRQRST
                     ITK"
BASE COUNT         8548 a        10481 c        10416 g         8836 t
ORIGIN      
        1 cgcggcaaag ctcgacctgg atttgattat ccgagtcgcg ggaagggatc acaattttcg
       61 cgtaataacc agaggcggca tgaccgcagt tgacgatcat cgtcttgcct tgtcgttcaa
      121 taccacgtcc ctcatggaca tgaccacaga ggacgagatc cggctgtgcc gttgaaagaa
      181 actcttggac ggcacgactt cccacatggc aaccccagat gacccggtcc acaccgcatt
      241 gccttggagg tacatgggtg aggaggagat gaaccgagct ccctgcgatc tgctgaaacc
      301 cgacttggag atatgaagcc agctcgtctt cggggaattg atacatcccc gggcgccatg
      361 gtggaatgcc ggacagaccg tggattccca ccccattgat caccacacca cgtcctgcta
      421 gccccactcc taattcgttg agccgctggt cgatggaagc actatcgcaa ttgccggcaa
      481 cagcgaacac gtggtcagca tgagatcggc aaagttctat gatctgatcc acatccttgg
      541 gtgatccaaa gtgagtgata tcgcctgcta gggcgacgat gtcacaccga tcaacctgat
      601 ccagtattgc tgccagtgca tcgattcttc cgtgcaggtc agaaatgccg aggattacca
      661 tcgcgcaaga taaaatcggt ggcagaaggt gtgtgacagt caagaatcgc tgaagaacaa
      721 tggccgtatc atggttatca tagggcaatc acggccagcg acaagttagg acggatgaac
      781 cagggggctt atcttgttac aaaacgtgtc ttcaggcgtt ggttcgtgag gcccaggaaa
      841 caccaatccc gcttgaaaaa gacgcggaga cgtgagacca ttgagcaaga agggttgcaa
      901 accactttgg gttctgaggc agaagcggcg gattcatgaa gatcacagcc attgaccttt
      961 atcttctttc tttgccatta cggacgccca ttcggttcga gacggttccc ttcagtaccg
     1021 acccttgtga atggtctcgg ctggagacgg tcttagtccg gctgtggagt ggaggagcct
     1081 atgggtgggg cgaggcgtgt cccggcaacg gtcccttttg gtcggcggaa tgggcgggtg
     1141 ccatattcta ttgcttgcgg gattggcttg ctcctcgcct tatcggacgt gaatacgcaa
     1201 ccccggaaga acttcacgag cgagtagccg tgttccgtgg gtggcgagcg gccaaggctg
     1261 ccctggatat cgctttttgg gatttgaaaa gtcggctaaa taaccgcccg ctctatgcag
     1321 aattggggtc ggcaccacgt gaactccctt taggaaagag cattgatcgt ccgggtcatc
     1381 tgacgactga tgagttcatt aaagaccttg ccaatgcctt cgagtcgggc tacagccggg
     1441 tcacagtcaa ggtgcgtcca ggctgggacc ttcagatgct caattttgtg cgacacgagt
     1501 ttcccacgca ggatatccat ggagattttg agggggcttt aacactgaat cacatggaga
     1561 ttctctaccg ccttgacgac tttaacctgt gtatggtgga acaaccgttg cctgccgacg
     1621 acctggtggg gcatgcaatg gtccaggagg ccttgaggac ccctgtttgc ctggacgagt
     1681 ccattacgac tcctgcccaa gccgatatgg cccttgagct acacagcgcg aagtatctca
     1741 atgtcaaccc gattcgggtt ggggggctca caccggccaa ggccattcac gatgcggccc
     1801 atcagcactg cgtgccttgt tatttgggcc tgtacccggg aacaaccatc tctgtgagag
     1861 ctgcccttgc actggcgtct ctctccaatt tctgctatcc gcccgattat tgggacactg
     1921 cggaggcctt cgtggaagac gtcgccgagc ccctccaaac agtgagaggg gatcagaatg
     1981 gccgactgac ggtttccttc tggtcagatg cgggtctcgg tgtcgatcct gccgcagagg
     2041 tcctgggcaa gtactgtctt tctgaatgcc atctcccgta agttgtgcaa ctctgttaac
     2101 ttcgagcgac gagaggatgt gttcatgatt cacgtgattg cggaaatcgt caccaaaccg
     2161 ggacaacgcg acgccgtgct tgcggagttt cagaagatcg ccccggcggt gcgacaggag
     2221 cccgggtgct tggaatatgg ggctgccctt gagacagccg caggtttgcc ggtccagcca
     2281 ccggtccgtg ataatgtcat caccgttatc gagaagtggg agtctctgga actactgcac
     2341 cagcacctcg gggctgcgcc attacagtcg ttcttgcaaa agacccaaca tatgattgaa
     2401 tcattgtccg cacgtgtcct tagcccagtg ggctgagggc ttcgctgtcg tcagagccgt
     2461 cttctgcaat cgagctgaga ttgccaacag tattcaggcg ttgagggtta ttgacattaa
     2521 ctcaacaaaa aaggcggaaa cgatggaacc gtttccgccc gactgttttt ctttcgcttt
     2581 gtgggggact aaaagcctta ttcttcctct tcctcttcga gtttggcctt ttcgataatt
     2641 tccttttgga tatttcccgg gacgatgtcg tagtggctaa attccaaggt gtagctaccc
     2701 tggccaccgg tgatgctcga caaggcccgg gcgtacgtga tcacctcggc cagtgggact
     2761 tcggctacga ttgtttggaa tccacctcca gccgaggtca tcccaaggac ccgaccgcgc
     2821 cttcccgaaa gatcgctgtt aatatcaccg accttgtctg ccgggactgt tacctcgatc
     2881 ttcacaatcg gttccaacaa ggccggtttt gcctgttgga aaacattgcg gaagaccatc
     2941 gaggcagcgg ttttgaaggc ttgctcggag ctatccaccg ggtggtgttt gccgaagaag
     3001 acttccacac agacgtcctg aaccggatag ccggcgatga cgccacggct gattctttcc
     3061 aagaagcctt tctccacggc gggcataaag ttgctgggaa tggtcccgcc cacgatcgag
     3121 tcgatccaga ggaagtttga ttcctcgtga taatggactt ctttcatttc ggggaaccgt
     3181 tcttttgtgg cgaactcctc caccttggtg ccccgcggga aaggaaacat gcggatgtgg
     3241 acctcgccaa actgcccacg accgccggtt tgcttcttat ggcggtacat tccctccgcc
     3301 ggggcctgga ttgtttcgcg atagggaatc ttcggctctt tgacatccac ttccaccttg
     3361 tcacgccgtt tcaggcgttc gcggatcacc tggaggtgca actcgctcat tcccgtgatc
     3421 accaactcct tggtttgggg gtcacggtcg agccgacagg tggggtcttc ctgacaaatc
     3481 ttcgctaagg cgccagagag ccgttgttcg tccccgcggc tcttgggggt gattgccaag
     3541 ccggtcatag gcgtggggaa ttcgataggt ggcatcacga gatccccgat gctcatcccc
     3601 gtcttaagtt cttcgacctt ggttacggca acgatcgcgc caggcccggc ctcctcgatg
     3661 gggatcatct ctgaagcctg cacatggaac aactgggcca acttcaggcc cttgcgttcg
     3721 cccaccaccg aaactgtcgc gttggtcctc actgttcccg agtacgcccg caaaaagctg
     3781 atcttttgca cgaagggatc aacccgcgtc ttgaagacct gggcaaccag ggggccactg
     3841 ggatcggcag ttagctcgac ccattcccca ctgtccggtt tttgggcacg ccggacgatt
     3901 cgatcgggcg aaatgccaca aagatcgaat accttaagaa gctccggaag tcctacccct
     3961 gtcttggccg aaacacaaac gatcgggatg acggacccgt caatcatggc gcgacccagc
     4021 agttgggaaa tctcctcgtc cgttggcatc tggccttcaa aataacggct taatacctcc
     4081 tcatccgcca caacaatcga ttccatcaaa gactgactga tcgaactggg gtcaatgacc
     4141 gcgccgggaa ccgatgactc ggcctttagg gtcgtggcaa caccccgaaa atcagcacca
     4201 aggccaatgg ggacattgag cgggacgcac cgatcgccga aggtctctcg aatacctgcc
     4261 aagagatttt cgaagtcaat attttccgcg tcgagcttgt taatgatgat caatcggggg
     4321 attccagctt tttctgcctc ctgaaaaact cgccgcgtgt tgacctcgac acccgactga
     4381 gcgttaatca caatcgcggc caaatccacc gcgcgaaagg ccccgatgac ctgcccgatg
     4441 aaatcccggt aacccggcgt atcgatcacg tgatagtgga gtccgtcata gtcaaaaaag
     4501 gtgacctttg cctcaatcgt gtaacggtgt gacttttctt cctcgtcgaa atcgcagatg
     4561 ctcgtcccat catcgacgct tgcgggtcgg ttgacggccc ccgtgaagtt cagtaaacga
     4621 tcgagaagag tcgttttacc aacggaaccg tgaccacaca atgcgatatt ccggattttt
     4681 ttcacttcgt aaagagccat agaaacctgt cctcaataac cgattctgcc agggtccaca
     4741 tgcgattccg aaaaaacctg ccccaccatt ggggcacgat agctaagcga cctttatttc
     4801 taaccgtatc tatttactga caggatcctt tatcctcagc gatggcaagg acctcgggta
     4861 agttcaatcg tccctcgtag agggcacgtc caataatgca acccgttacc ccaatttcct
     4921 taagtgccct gatgtcctca agtgttgtca cgcctccgga tgcgattaac ggcaccgtga
     4981 tgacctgctg catgtgccgg aaggcctcaa gactcacacc ggagagcatg ccgtcggcac
     5041 taatgtctgt gaaaacgacc gccgcgatgg gctcgtccgc aaactccgcg acaatctctg
     5101 cggcggttcg gttggagatc tccaaccacc catcggttgc cgcgcgaccg gctcggacgt
     5161 caagtccgag gacgatccga cggggaaact tccggacgac cgccctgaac caatccgggt
     5221 ccttcaccgc ccgagttcca acgaccacgc gcgatgctcc agcatccagg aggcgcgaaa
     5281 ttgtggtttc gtcccggacc cctcccccaa actggcaggg aaccccactc tcctggaata
     5341 tcctctgcac gatgtcgaag ttgacaggct cccctatttt cgccccatca aggtccacaa
     5401 tatgtaggcg ccgcgcacct aacgtgaccc agtggtgcgc aattgcgaca gggtcggaat
     5461 taaatacgat ttcccgaccg taatcgcctt gacatagcct gacgcatttt ccgccacgaa
     5521 tatcgattgc aggccagact tccacggggt ggcccttcaa ctgacgcgct tttttccaac
     5581 atcgtaaaac gtgtaatatg acacacagga gctagtcctt caagacacgc tgcgaaaagc
     5641 ttagaaacaa agaggagaac cgggacctca aaaccttctg ttagacgaca acagactgat
     5701 ttcggtcggc tgcatgggga gtggaaaccg acaaggaggt gctccttgtc tatgcgcaga
     5761 agctccttgt ctatgcgcag aaagcgctga gatgttgagg ttagaagcct ttcccaaaaa
     5821 gcttcccctc tccctccggg agagggttgg ggtgagggcg agcgaacgtc ggaatcttgt
     5881 tgagaaaacg ggtagcgatt cgagccactc tcacccccga cccctctcct gccgaacggg
     5941 tgaggggaga ttatgggata ggctctaaaa ccggcaaatg ggtttcgtcg gacgcgcgtc
     6001 aggccttcct tgacccaggc cccatcgtta ctcaacgaca ggaggtaggg gtcgaggtgc
     6061 tgacaagctc ggccaatcag acacttcaag cgtattctcg tcaagaacgg gcggtagggg
     6121 ggcaacttgg tgggcggact gaaatgctcg cggggaagtg ccggccggac ccgagggagg
     6181 taccggtagg ttctggtcgg gcaatgcagg tctttcgact ggtgttggca gctccacatt
     6241 ggggggcatc tggctttgct tcgacgcgtc cgctgatctt tgggaaaggg cgtctagcca
     6301 ctgccgagcc tcttggaagt ctggcttgac ttggactgcg tgggcgaaat accgcgaagc
     6361 cgccgcatgg tctccctttc tgaccagcaa atagccaaga ttgtaatagg cagtggcttc
     6421 gtcatgaacc gcttgaagct gctccaacgc cccttgcaaa tcgcccattt gcacgagcac
     6481 cgcggctagg ttgtgccggt accgggactc tttcggtgag aggctgatcg ccttttcgaa
     6541 cgcggtcttg gcatggtcaa gccggttcat ccgggcatag cacaagccca ggtgattcca
     6601 agccgtcggt tcccccggat gggctgtcgt tgctttttgg tagcaggtga gggcctcctc
     6661 caattgaccc tgcctgtcct taagacgccc taacccaaga agcgcgtcca agtatttggc
     6721 atccatactg agggcttttt cataatactg ttcggccatg gcaggttgac gagattcttc
     6781 cgcaaaacgt gccatagcaa cgtacagccg gggactcggc ttggccttgg tcgatagggc
     6841 tgttgggtcg ggagcctgtt ggaccggctc cgtgaacgtg agtagttccc cgaccttttc
     6901 caggccatcc ttaacgctac tgagtgggtc aaattgggcc gaaccggcgg tggtaacacc
     6961 tcccgcggca accacgccgc tctttcgagt gttcccagtc tgagctgtac cggttcccgg
     7021 tgagattatt ccgagccctg tcggaccgga ttttccccca agggactgct ttgaaggatc
     7081 agacgcgcag ccgcaaatag taatgcctag taatagaagg ctaatcggga acgcaacgca
     7141 caaatttccg caatcctttt ggcccatgtg caaccctccg tggtttgtgg gcccggcgtc
     7201 atctctcgta tgttgcccag tttcgcgcgc aaggtagcgc tcctgactac cggcaacagg
     7261 cataggagtc atcggcaggc ggcgcagaaa aatcaagaac aataggagct aaccaaggta
     7321 aactcatcat gaaagcccat gcagggcctc cattttctct actttgtgca cctttcgatt
     7381 gtgtatgggg gttttcgtaa taagtatcca cgagtcaagt gaaaggtgcc gataacacac
     7441 gaagaagagc cacggaacgg tggatgatac cattcccact gcggaatttg gtgattcagt
     7501 tatcacgcat gaggacaagg aaatgtcgag gtccttcgca cttctcgttt gtagtgtaac
     7561 tttatgggcg tatacagcct attcggattc cgttttcgcc caaggtgtga agggattctt
     7621 tcatagcatc aaggtcgatg ccgtccgcaa tgcctgttgg cccgatgcgt tcgttccggc
     7681 agaccgcacg aatgcccgtc tgccgtttgt gataatggtt cacaatggct ggaaacgaca
     7741 gaatctgatt gcggaccact acttcgcgga agacggaaag acgttaaccg atgctgggaa
     7801 acggcaggtt cagtggatcg tgacccaagc accgctaagt caccgggcaa tttatgttaa
     7861 ggaaggcgaa gacgccacca ttacagaggc ccgtgtgaag gccgtgggcg aggtggcgag
     7921 caagtacctt ggacctgagc aaaagcccga cattcgcgtg accaacatca gtccacccgg
     7981 ttggccggct gagctcgttg acactgtgga aagggcttat ctcaaaacga tgcccgaccc
     8041 cagactgcca gcccgctctt caaattcgga agcccaggtg gagctatcca actgacagcc
     8101 tatcccaagg aatctccctc tccttccggg aaagggttag gatgagggca agcgaacgtc
     8161 ggaatcgatt gttcgcacgg gggaataact ttcagacgga tggcctggcc ttgactatag
     8221 cttgacggct ctttttctca tttagcgctt ccatctgttt acgcagagaa gggcatcgct
     8281 caataaggtg ggcgatgtga tgaccgtcac actcaggccc aagggtatcc acaatgattt
     8341 ggtgaacttc ccagtcgtct tcatccacca agaccaagcg aatatcgtct cggtctaggc
     8401 ccggtgggat gggaagccaa tgaatcttga atctttctgg gtgggaataa atgtaacggg
     8461 tgggatgcgc gcggtccgaa ggatttcgca accgttcgca gaggcggtga agtgtcttgg
     8521 tcagcaccag ctccccgtac acaggaacac tggttagcat cgctggtgtg ccgttatggc
     8581 ttcggtaacc gacataatca gcaggctcat cgagacggtg ggcttccttg accaggcgat
     8641 cgaccagcag ttgatccaca aagggacacg ccgtctcaaa ccgcacaaac tcgcggcaag
     8701 gataagcttc caaagctttg gcccaaattg tcaaatgatc gtcgccttcc ccgacgaaca
     8761 ctggcacatc aagcggaaca aggtctttga caaagaggtt tgctggatct gacgaggtga
     8821 cgacaataac gccgtccaac tgttggcagt cggttgcccg gcgcgtcacc cattccagaa
     8881 tagactttcc ttgcaaccgc ccagcagccc ggcgtctttc cttatccgat gcaaaacatg
     8941 catccacaat tccgagtgtt tgaagcatcc ggcgcaacct ccttcaaaag tcctttttga
     9001 tagccaagca ttccttgatc tcactcgccg ccggaccagc ggtgtcaact ctgcttcaat
     9061 atacccggct aacaatctcg cagtcaacca tcacgggaga aaagggacgt gtgcctacat
     9121 tcaacacaaa caaccttcat tggagcattc acttgttgga tttacctgat tgcttgggag
     9181 ccatctgcgt tcgcatccac tcttcgagtt tttctcgaaa tgcctcgggg tcgtcctcgg
     9241 gccgacgggc gggcgcggcc tgcttcgtag tatgacgctg gatactggga cgcggcttct
     9301 tggactcagg ggtagccttc tgttgaggtt tcctccatgg agcnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnncgctccg cgagggtccc atcagggaag ggtggggtcc
     9481 ccaccaaggc aaagtaaagg acgcatccga gagaataaat atcaacccgg tgatcagctt
     9541 ggtccggagt aagggctaac tccggcgcca tgtagtccac agtccccagg acttgggacg
     9601 ggtcctcttg tgaaacctgt gtaccacgat cccgcttaat aagccgagcc aggccgaggt
     9661 caagaatctt aacgactccc tccgaattga cgatgatgtt ggccggtttt aggtcgcaat
     9721 ggataacgcc ttgagcatgg gcatagccta agcccaacgc agtttgtctt gcgatgtcag
     9781 tcgcttgcct ccagggcagg ggacccgact cgcggaccat gtgctcaagc gtcttaccct
     9841 cgacatactc catcaccaag tagtactggg ccccatcaca atcaacgttg taggcatgaa
     9901 caatgttagg atggtccagc gacgcaattg tccgaacctc ggcaagaaaa cgatctctgg
     9961 ccgatggcgg caaattcgag gagatgagct tgatcgccac acggcgctcc atggtgacat
    10021 gttccgcgag gaagacgtgg gccattcccc ctcggcctaa ttccgtgagc agacggtact
    10081 ttccaaggag aaaactttgg cgtcctttga ctagttcctc cgcttgccag gcggtaagga
    10141 ccttttgacg gactagatac tccagaaact gcgcaaaact ggtgatttgc gagacctcct
    10201 cgctcatttt cggcagatcg cacgacaagc cagcggcggc gagccggtgg atccattcct
    10261 gaatttgtgc ttcggaggcc atattcgccc tgcctcaaaa ctcatcaaca cattgatgtc
    10321 tttgatcgcg ttccctcaaa taatttgctc catcggtcga ggaactgatc tcgcagttct
    10381 tcggccgtcg tggtcgttgt aactgccagg gaaacgacgg ggatcccaag acctcgatag
    10441 taatcaacaa gaggccgcgt ctctgactcg tacaagcaat actttttggc caccagggat
    10501 tctaagtcgt catctcggcc agtacgatcc cctcccacat tggagtggat ccgttcgaag
    10561 accacgtcca aagtacattg aaggttgacc acaagttgaa ttggcagaag ccttatgatc
    10621 ctttcagctt ggaacagcgt acggggtaac ccgttaagga tgataacctc catacgatcc
    10681 gagttgtatg gaatcgctga caaaaaagac cgcaacaaac gatccgcgat gaagaattcc
    10741 tcatctttga gcagacgccc agaggcgagg atgtccgcaa cgaggtgtct ctcatcgtcg
    10801 tcaaaaagac attgcgactc ttgatctgaa atgactatac gaagccaatg tccgaagtca
    10861 aaatgatgaa atcgccatcg gccatgtccc caagccgaaa gagactcgcc aagcggggtc
    10921 ttgccggctc cggtaggccc aagaaggatc acacctgtgc gagaagatga tggcgtcatt
    10981 gtggtactaa acaatctgtc gttaacatgg gaactggaca ttcctattct aagacttacc
    11041 cgcgtggaaa gcacctttgg ttaccccaca atgtcaggtt gagaaacgtc tgtgggggac
    11101 gtgaaaacgg caatcgaatt taaagtcctc gggcggccta ttttgcgact cggttgactc
    11161 ctgggatgtc gcgattccat ctgtaggatc cttcacgggc agcgacatca tgggtatgat
    11221 ccgtctatcg ccttggaaca cggcaatgct gcttgtttat gttgatgcgt ctttttcgca
    11281 ctactgagaa agcgccgcta ctattggcat tgtggtgatt gtcaggcgaa gcatgccctt
    11341 tgccccgatg acaagtcggc actgcacaca tgaagttcaa aatagcattt gccataactg
    11401 aattggaaat gggcggggcc gaaaaggccc tcaccgagct ggtacttcga attgatcggg
    11461 caagatttga gccctgcgtc ttcgtcctct cggtaccccc gctcgaccaa atcctggtaa
    11521 agcgccttac atcgcacaac gtgcccacgt acttcctagg gttgcagcac tggcatcagt
    11581 tgattccggt catcgttcgg ttcgcccgca tgttacatca agaaaaggtt caaattctcc
    11641 agtcgttcct ttttcatgca aatatcgttt cgcgcattgc tgctcgcttt gccggcgttc
    11701 cggtcgttat ttcgggcttg agagtcgctg agcatgcagc acgttggcat gtgcttgtgg
    11761 attgtatgac cagcgggtgt gtcgaccact acgtttgtgt cagtcaaagc gtggcccaat
    11821 ttgcggtccg caatggtctg ccacctgaga agattcgggt tattccaaat gccgttgatc
    11881 cacctgaatt ctgtcaagaa agtccacaag gaacatacgg cgcggataag tttttgcccg
    11941 aacatattgg acgcaatgag caaaatacca cggcaccgtc gtgtggtcga acggctgctg
    12001 gatcggaata ccgcatggtg acggtagcgc ggctcgagta ccaaaaggga gtcgactggt
    12061 taatcctggc tctcgctcgt tggcttaggt cgtatcctga tctgcgactg gtgatcgtgg
    12121 gagacggccc gatgaaatca acactgcagg cgttgattca cgcgcggggg ctctccgagc
    12181 aggtgacgct ggtcggtttt caaagagatg tggcaagaat cctttgtgat gccgatcttt
    12241 ttgttttgcc ttctcgttgg gaaggcatgc ccaatgccct tcttgaagct atggtctgtg
    12301 ggctgcccgt cgtcgccaca gatgtcgaag gtgttcgcga aatcttgggg ccttgcatag
    12361 cacaccaact tgttccattc ggagattccg atacgctcac gcatgcaatc ttgtggcatt
    12421 atttgaaccg ggattgctct cagacgattg gacaacaaaa tcgcacgtat gtgttgtcgt
    12481 cgttttcttg gccgcgtgtt gttgagcagt atgaaaagct ttggttgtgg cttcttcagc
    12541 ggggataatt gaagcgaagc aatgcgtgag cgctggggta aatggactgg ggggaccgac
    12601 gaaatgggac acccactttt ggagaatgcc ggaccaaccg cgcacaaaaa gtacattttt
    12661 tgccgtatcg ggtgaatggc taccgtttac tactttgacc ggcccatgcg ggggacgtgg
    12721 gcgccttgtt cctcggttcg gcgaggaaaa ggattgtgtt gaacacgaga actaatcggg
    12781 ctttccgctg ctacgggaaa ttctgggggc ggatgatatg gtagctacgg gcttcgtagg
    12841 gggaacaaat ggatgcactc gatgggaggc catccttaat cgtgcggggg acatccctcc
    12901 ccgactggtg gctccaggag accccgctca aaagcggccc ccggtatatt gcggtacctg
    12961 ccgttcgctc gtcaaaaggt ggtaccgtaa cgattgacgg ttatgacaga atatccgttc
    13021 gaggggctat gtaagcggaa ctctcaattt actgaaattc atgttacgtt gaagaaactt
    13081 gccttgtcag tcaagagaaa ctataaaaat agtcgacggc ggacggcgtt cgttagccag
    13141 ttttgtcaac ctatcgggcc acggttcgga tcgaaaaaat cgggcggata acatcaatat
    13201 gccatttgac tatgggccgt gatctgcgca tcatgaaaaa cgctgggttt tggaatgcgg
    13261 agacaggagc taactgacag cggtcaatgc ttagatcagg tgaagacgtc cgtccgagct
    13321 gtcgcatgag gtcggggtag caaacgttgg cgacttggcc accggccaac gttcacccgg
    13381 ttctgccacg tgccggttta ccccgccccg cctgatgtca gggacgtcat caggagcgac
    13441 atgcgagccc cagccacgcc gtggagatac ggggctcaag ggcctatcct ccgaggatcg
    13501 taggaatgcg aaggaggata ggccttttct tttctgttcg ggtaagaact cttctggaac
    13561 atgggctttc cattgatcgc gagctgctcg ctaaagtgac caaaacccta tgcgtgcatt
    13621 gtcgagaagc aagaactata atgggcgaca gttgcgtgat ttgcaggcga cgtccagtgt
    13681 cgtggatttg actcagcatc cgttggggag gtgtaccaat gtctcttcaa acaaactgcc
    13741 tcgtggcgtt ttctgtttgt gtgcttgtcc atctcgcggg aacggtggca acacccagtg
    13801 aagtcgcaaa accgaacgtg attgtgatca tggctgatga tctcggatac gaaaccattg
    13861 gggcgaatgg aggcacttcg tatcaaacgc cccatcttga ccggcttgcc gcaacaggtg
    13921 ttcgctttga gcattgctac gtgcaaccgc tgtgcacgcc cacgcgggtt caactgatga
    13981 caggccaatc caacgcccgt aactacattc ggtttggtgt gattgatcca agcgtcacga
    14041 cctttgccca tctttttgaa cgggcgggtt atgcgacggc gattgtaggt aaatggcaac
    14101 ttgggcgcga agtggatttg ccgcggcgaa tgggatttca agagtactat ctctggcaac
    14161 atactcgccg accgccgcgg tatgccaatc cgggattgga aattatgggc gtcgaaaagg
    14221 acttccaaaa tggggaatat ggtcctgatt tgctgaatga ctttgcctgc gattttgtca
    14281 aacgtaacaa agacaagcct tttctgcttt attatccttt gacgttgacg catgcccctt
    14341 accagccagc gccagatagc cccgattggg acccaaacgc tcagggagaa caggttaatc
    14401 agcatccgcg tcattttggg gagatggtcc aatacatgga caaattgatc gggaaacttg
    14461 tggatttgct tgacgagcta aacattcgta agcacacgct gctgattttc ctgggtgaca
    14521 acggtacagg cgggggtatc cgctcaatga tgggcgatcg ggaagtgatt ggcggcaaag
    14581 ggaaaaccac gcgggctgga atgcgtgtgc cgttgattgt gaatgggcca gggatggttc
    14641 ggtctggagt tgtctgctct gacctggttg atagtacgga catttttccg accatctgcg
    14701 aggccgcggg aatacctatc cctgacggtt tggttctcga cggcaagagc ttttggccac
    14761 aattgtgtgg tcagcccgcg acaccgcgaa aatggatcta ttgttggtat tctcccaggc
    14821 agggagccga tacccgtgtc acggagttcg cattcaatca gcggtataaa ctgtaccgtg
    14881 atggccgttt ctatgactgt gagttggatc cagaggaaaa gcaacccctt gaggatagcc
    14941 aattatccga ggatacccgg cagatcaggg aaatgctaaa gcaggtcctc gatgcccatg
    15001 cagggattcg gcctgcccac ctcgacgagc agttgccacc tccggatcgt ccggcaacac
    15061 gccagcgacg ggcacggcag cagtaaccta aggacttgca tgtcaccact ccggaggtta
    15121 agttaaaccg gcataagaaa gtcttggcga agtgaagcat cgtaaaggtg tctcagtgcc
    15181 tcaccaatga ttcaggtgag gtgtgggcga tgattaaagg aaaaaaggaa tggctcggta
    15241 tcttctgttg ggatttgttg ctctttccac actgacggtt cgcattccag cggcagagtt
    15301 gcaattcgag ttgcatcgaa tcggcagttt tcggagcgag gcctgcggcg tggccgactt
    15361 caacggtgat ggtaagttgg acgtcatcgc gggtccattt gtttatatcg cacccgattg
    15421 gaaggcatac aagatccggg agatcaaagg cgaggttgac caggcaggga agggttacat
    15481 gtggaacttt gcggatcttc ctctggacgt agatggcgat gatcgcgtgg acgtgatttc
    15541 gtgtgattgg ttcgagaagt gtttagactg gcacaggaat gtcgggttcg gaggcgacct
    15601 ttggcctcgt agcgttattg atgtgagtct caatcacgag accgcacaac ttgtcgatat
    15661 cgacggggat gggcaaaagc gcgaaattct gccggaagtc gttgaaacat actggtttga
    15721 aatcgtgcgc gatccaacgg gaggtgtccg gttcgaaaag cacattatca gccaaaagaa
    15781 aatgaatttc ggcggtggcg tcggtgacat caatggtgat ggccgaccgg atgtaattcg
    15841 gcctgatgca tggtttgagg cacccgccga tcctcggagt ggtcaatggg tagagcatcc
    15901 aattgccttg ggcggacggg aagaggggca atcggaacac acggcccaaa tcctcgtcca
    15961 tgacgtgaat ggcgacggcc tgaacgattt aatcgccagt tcggcacacc ggtatggaat
    16021 ctactggtac gagcaggttc gcgaggatgg gacgattcgt tttcgacgac atctcatcga
    16081 caacacttgg agccaggccc actcgctgac actggctgac ctcgacggtg atggcgactt
    16141 ggatttggtg acaggaaagc ggttcatggc acacaacggg agcgatcccg gggagttcga
    16201 tcccttggga gtttactggt acgagttcga ggccaaaccc cagcccaaat ggatcaaaca
    16261 tgccatcacg tatgatcaag gaatcggggc gggtttgaac attccggtag tggatttgga
    16321 tggcgacgga gacctggata ttgtggtcac gggcaaatgg ggtggcccgg tgtggtttga
    16381 aaacaaactc aaatagggca cgctattaac cgacttgaca agtgcctgaa gatgtggctt
    16441 attttcgcga gtgggttcgc tcatagcaga taattccgtg aaataatcgc cggctgacat
    16501 catttcgatc ggctcaattt tatgacgggg agattgtcaa tggataggca aagaattagt
    16561 actcgacgcc acttcctgaa ggttacagcc ggactttccg ccggtgcact catgccgatg
    16621 attgtgccgc ggcacgtcct gggaactgaa accacaccat cggcgaatga aaggattggc
    16681 attgggggaa tcggcattgg tcgtcggggc ctgcaactgt ttgaggagat tccttcgctt
    16741 ggtcgagttg ttgccattgc ggatgtcaac cttccacggg cggagaagac ggccgagcca
    16801 taccgcgcgg ttgcatacca agactaccga aagcttctcg aacgcaagga tgtcgatgcc
    16861 atcatcacgg ccacgcccga tcactggcgg gccattgtct gtattcatgc atgtcaagcg
    16921 ggcaaggata tttacgctga aaagccaatg agcctcacaa tctatgaggg gcgaaaaatg
    16981 gtcgaggccg ttcggaaata cggacgggtg tttcagaccg gcagccagca gcgctctatg
    17041 gcggctaacc gcctgggatg cgaattggtt cgaaatggtc gaattggcaa ggtccacacg
    17101 gtgattgcgg ccaactatcc tagtccttgg gaatgcgcgt tgccgggtca gccagttcca
    17161 gcgggattgg attgggatat gtggtgtggc ccaacagagc cggttccgta tcacattgac
    17221 ctgtacacgc cccgggccaa tccgggatgg atttcgttcc ggccgtactc aggcggagaa
    17281 atgacaggtt ggggggccca cgggttggat cagattcagt gggccttggg taccgatgac
    17341 acaggcccgg ttgagatttg gacagagggc ccgaaattct ctccgccaac atatacccag
    17401 ccggagtccc gtgcccgggg cgataagatc tgcagtcagc cgaaagtctt ttttaagtac
    17461 gcgagtggcg ttgtcgtcaa atttgacaac ggaccgccgg gcggggcgat tttcatcggt
    17521 gataagggcc gaatcaccgt tgaccgtgcc aaattcacgg ttgagccgaa agagttgggt
    17581 gaccccccct atgacgactt ttctgttcga ctttatgtca gcaacaatca catgttgaac
    17641 tggctggagt gcatcaagac ccggcagaaa cctgtggccg atgtggaaat cggacaccgt
    17701 tcgacaacgc tgtgtcatct gggtaatatc gcccgatggg tggggcgacc tttgaagtgg
    17761 gacccggtga aggagcagtt tattggcgat gatgaagcca attcgtacct tgaccgtccg
    17821 cgacggaagc cttatgtctt gccggatccg gtgtgaaatc tcggcggctt tttgatgtct
    17881 tgcacatggc ctagcgcgct tttttgcgac ttccgttggt gggtcttttg cggggtcgca
    17941 ggatggcgat gacaattata ataggaagta ggagtaagag ggcccgcggt cggaacaccg
    18001 ccacagccaa cgctgccggg atgaccaatg ccagccaggt gggaagggca agaatgtggt
    18061 agtaagccaa ccaaatgagt gccgtcaaag gagccatccg ccaacatgcg gcttgacaca
    18121 tgatctcgag actactgctc ggagggcgca gttcgaaaaa aacgatgccc gcagccagga
    18181 ccagcgtcag aatggctagg ccatgccgac ggagtcttag agacttgagg gtttcctgtg
    18241 ggctttgaac gcggcgagac ttcatttcgc tcctgggttg gtctcctcct tcacaagagt
    18301 gacgccggct gggctgatac ctggctactg acgtcggtgg ctatgcctta gtttacgtgg
    18361 cgaatggttt accagcgcag tcattacgat cgagccacct tcagggggct tgtcgtatga
    18421 cggagagcac cgccgaccac tcggcaatgg tctcacctat acgaaaagca cggtgcccca
    18481 aaatgccgtt caggtacagg cgatcggttg ctcgtccccg ttcgggcttt gtggctgcgc
    18541 gataaacaac tctccggtga ggcgaaatct caattctcaa ggcgttgtca acgaggacct
    18601 gtgtaattcc ttctctgtcg atcttttcgc cagaatgggc cggaatctgc catgtttcac
    18661 cgttcaaaga aagcgatcgt tgacccgtct cgtagatcca attgacgttg tttaggacgc
    18721 caatcagacc ggtggccact tctgcggtga cacagtcctt gattgccacg agtttctctg
    18781 cgatttcaac ggtgccagac gcgtgtgacc tcatcgtcca ctctgccgcg acagcgtcac
    18841 cgtggtgtac agtcaatttt gctccgaaac cgtcaggatg tgtggtgact tcgacgctct
    18901 gaagtttgat cggcagcgct gccgtttgac cgtccaaaat cacatggccc aaaaggcttc
    18961 tttgatcagg agacgtcaac cgatcggggc gattcatcgt gatcgttccc atgattatgg
    19021 gcccgtaact caaggcgcaa acgaaatcgg gagttcgatg caacacgagc ttgccaaatt
    19081 ccagatgccg aacgcctaga cgtggtcggt attgacccat actttgcggg tcagcgaagt
    19141 gcagattgag ccacaccttg gctagggctg cagcaatgtc cgtctgcgtg gatggaaaga
    19201 agaactgcga gggatcgtaa atcgcaccca cggaagaacg tcgttgcatc cgctccgtga
    19261 catcaagtgt ttggttcagg agtgcccatg cttcgggatc tctcccaaaa acgaacatga
    19321 cgttatgaca ggtgatccat gccggttggc gaaaaatcgt ccagtcctgg ccactcggat
    19381 aaacataacc catgtctgga agaaggaacc acttgaggtt ctcataaatc ccggccgtat
    19441 tccagtaaac cgacgccggt tcttttcgtc ccgtcatccg gaagtctgtg acacacccca
    19501 gggagagtgt gaaggttccc atgtagtcgg gatgcacaaa tccgtgattt tccaaggtga
    19561 agtcgtcgta aatattggct ccggtgaact gctccgctac gggacgtcca tccacgattt
    19621 cctggcaagt ctcatccgca ggacgcaaga aggctgacat cgtccaccgc tgaaactggc
    19681 gatcccactc tggtgcccgc tcgtcattcg gcatcagtag catagccacc gagaggattt
    19741 gggcgttcca ggcgttttct tcggcctttg tatctccccg caattgcgat ggcgggtcac
    19801 tccgcgcgat gcgttccgct tcatggcgaa ccacccggcg tacggcaatt tgcaaatctt
    19861 ccggaaggct gtcgaaaatc caccaggctc cccgacccag catctgggcc caatgggcag
    19921 actgccatgc atcgccccaa ggcttcccgt cgcccgtggg acgatttccc gtgacgtggg
    19981 tgtctgtgag atatcgaatc atggggataa tcgcctcatg gaggagtttc tcccgcgaca
    20041 tgcccacaac cgtttcatcg taggggccaa aacgataaag aaagcaaaaa gcctgcacgg
    20101 cccccgtgtt tggtcgaatc caatgctcct gacttcggct ttctgtgaga agtttgtact
    20161 tttcgtctcc attccaaggt tttagctgat ccagcaaaaa atgagcctgc ttttcgagga
    20221 ccagataaac ccgattagca atcgaggcaa tttctggtga tggtaactcc ggcaagtcaa
    20281 agcgcaagac gtcggcaccg tttgagagag caacctgtga tgagacgatg gtgagaaaga
    20341 gggccagcga taacgttgcc gcgaacatac gtccggcatg ccgttgacgc gataaagatt
    20401 tgccccgccg aagtaggtct cggtccatgc ggcaaatcct aattcaaaaa gggacacgta
    20461 gggataaacc actcggtaac actgccgatc attccgacag tcccattgtg ccccaagcga
    20521 gctaagggga caatcccttt tacggttcca ggacaatctt gccgacgagg ttcccggcac
    20581 cgccgagtgt gttttgttct tgcaaccgat gggcctccac tgcctgtgag agtcgaagaa
    20641 ccctgccgat cacagggcga agttttccat ttttcaacca ttgattgatc tgctcggcgg
    20701 cgcgctgctg ttcctctggc ggtgcattga acatggcgaa gccgaaggcc ttgcagtctt
    20761 tcacatagaa agatcccacc ggaaattttg gcttggattg acgcccggct attaagacga
    20821 gacgtccgcg catcgctagt agcgataccg ccatttccag attctgctcg cggagggttt
    20881 caaaccaaac attgaccccc tctggggcaa agcgacggat agcttcggcc tcgttttcct
    20941 gcttgtaatt gatcaccaca tccgccccta attggcggca caaatcggct ttttcctcgt
    21001 ttccggcggt ggtaatcacc ctggccccga cggccttggc catttgaacc acacaggaac
    21061 cgacgccgcc cgtcccgccg tgcacaaata cggtctcatt gggtctaagg ttagcatcgc
    21121 ggaatagtcc caaataggcc gtgatgccca caagggcaat ggctgcagct tctcgatccg
    21181 agaccgttgc tggggttgga tacagccagc attcatccac cgccgcatat tccgcgaacg
    21241 tgccctgccg ccccaaaagt ccttggtttg atccccagac acggtcaccc ggcttgaacc
    21301 gggtaacacg ggcaccgacg gcttccacgg tgcctgccac atcacagcca agaatgtagg
    21361 gctttggtag gggcattttc actgttcccg cccggatata tgtgtcaatg gggttgacgg
    21421 aaacggctcc gactcgtacc agaacctctg tttccttaag ttgaggcacg ggcaaatcgt
    21481 cgtaacaaat tacttcagga ggtccaggat ggtcgatata ggctgctttc atcgtcgaca
    21541 tcctttcatc gagtgaatgc ggagaactca actttccaca gtcatgcacc gccgggacga
    21601 atgtgcaggc aacagcatcc ttcgccgccg ggttgccaag tgtcggcgaa aaagccgaat
    21661 ccagcttcgc ggaatagtcc gtaatcgact tcggctgcaa tccgacagag tcgggcaaca
    21721 agctcgtcag gtaggccggc ctctacccag gcctcgcgga ggggacaacg gtgaaatttg
    21781 atatccagtg cgtcgggcgt gtccgtcagt atctccggtt caaaaagtcg gccatcgtcc
    21841 gggaggtggg ctaggaatgt ctctttgacg gccgcgagat tattgggggc aaatcgagcg
    21901 tattcccttt cccgctcctg accccgacga taaatcgccc gcttgagaat ctcttcggcc
    21961 tggtccggac ccaatatacg agccagttcg tcgtagatgt gatagtaaat gagtgcccga
    22021 ttcttgtagg aatcaagtaa ctgctgacgg agttgctcca gtttgtccgg ctgattcatg
    22081 gcagtgatcc tgatctggga aaggcctttt gagtaatttt gttctggtga aattataggc
    22141 tgacgcatcg gcagaaggcc actggaagtg catcactccg gacggatggc aggccgtctc
    22201 ctgtcctata agtgttccta tgaacacgta aaagcgtctt gacgatgtta ggatacaaat
    22261 gtacactaca tggggaggta acgaagatgg ccagcatcga tagcgctctc tctgccgaac
    22321 ttttacccat gccgtctccg gaaatcgtgg cacaattacg gaggaacatc tccactatcc
    22381 accgatctta ccgaccgcgc caggggcaga aattccctat cgacattcct gaggtcatgg
    22441 acctctttcc tgggggacaa ttaccctatg gttccattat ggaatggttt acagaaaccc
    22501 atggaacaga ggccgaatcc ctttcgttgc gaatcggatg gagtgcctgt ctgcggaacc
    22561 gatttttggt gattgttgat ccatgtttcg agatttatcc tcccggtctt tcccagttgg
    22621 gtatcgacct caatcgagtt gtctttgttc accctgcaac aaaatcggac atgttgtgga
    22681 ccattgatca agcgcttcga tgttcggcgg taggggctgt gtggtcctat ctcgaatcgc
    22741 ttgatccgat caatcatcgt cgttatcaac ttgcttcaga atgtggggga acagtgggaa
    22801 tttttgtgcg acctctcagg gcgaggaaag agtcttcgtg ggcagagttt cgccttttgg
    22861 ttcagccggt agcttttccc gccggagaaa tcggttccca atgtcgacgg attcaggtca
    22921 ccttgttgcg agctcctgga ctttcggagc ccattgtacg ggaggtatgg atcgatgacc
    22981 cagcggatcg cttgcttgac attcagcggg tggaaccttt ccaacgccgc taccagtacc
    23041 aaaaaaacag ggcataatca cgcttcttca cccggtcttc aagaagatga tgttcggaag
    23101 cgtcaggacc gcttggccga aatatgcctc cgctacactc cccatgtggg tttggttgag
    23161 gatccccatt cagattctct ttttcttgaa ttaacggata ttcagcacct catccccgat
    23221 gaacggcggt tcgttatcca gctgctgatg gactgccatc gggaaggtta tttccccacg
    23281 aaaatagccg ttgccgatac ccctggcgct gcctgggcca tgacgcattt tggcaccggc
    23341 ccaagggcaa cggagatttc ctcggcatgg attcttcccc cgtcggagag caaaaagtgg
    23401 attgcatccc tgcccgtcga ggccctgcgg atccctgatt tagtggccga gtggtgtcac
    23461 gagttgggcc tgcatcgtat tggcgatctt atccatttcc cgatggcaga attgcgaaaa
    23521 cgctttgggg agatccttgt cctgcgtctg gaacaacttc tgggaagccg gcaagaaatc
    23581 ctctcgccct atcttcccca gccacaattt gaggaacatc tttggttgga atatcccgcc
    23641 cgatcttggg aacacctaag tcccagccta agcgcttttc tcgagcgact tactcagcaa
    23701 cttatctccc acaacgtggg ggcactggga cttcgcgtca atcttctttg tgaggatcat
    23761 tccacggtga cactctcggt gagtttgttt cgtccaagta tcgatcctca tcacctttcc
    23821 ggattgatcg aattaggttt tagacagaaa catttttcat ctcccatcgt gggcattaat
    23881 gccgtcgtat cgcatgtggc cccgctagaa tcgcaccagg ccacactctt tcccgatgga
    23941 gagacggatt ggccttcttc agagtggggg catcttcttg aacgattgtc ctgccgactg
    24001 ggaacagccc gcgtgtgcta tgccgtccta aatcctgacc atgatccgga aaaggcctac
    24061 ttctggtacc cttgcctatc ccatcgcaaa tcccacaaac cgaggtctga cggtaaagca
    24121 ttggtcacaa agggctctcg tcctgtccgg ctttttcgtg ccccgatccc tatccaggtc
    24181 gtcacggcaa tcccttccgg ggttccttac cagatccatc atcaggggca atcacatcat
    24241 gttgttcgct ggtggggacc cgagcgaatt cagacaggtt ggtggcgggg ggagtcgatc
    24301 acccgggatt attaccgtgt tgagctgcaa aatggcgtgc actactggat ttatcgctgt
    24361 ttggaaagtg gaacttggtt cttgcacggt acttttggct gaggaaaagc aaaagggttt
    24421 tgaccacggt gttttcctgg ggaagctaca tttcaaggtg tgatgcgacc atgtattgtg
    24481 aacttcatgt caaaaccaac ttttcttttc ttcagggggc ttcgcatccc gatgagcttg
    24541 tccgtcaggc agccgagttg gggttagctg gcatcgccat taccgataag gccagtgttg
    24601 ccggcgttgt ccgggctcat gtgacggcca aggaactggg tgtcaagctc atcattggtg
    24661 cggaaatcgt ccccaaggat agtttgccgg tggttctttg ggccacggat cgggcttcct
    24721 acggccgttt gtgccgactc atcacaaaag gacgatgtcg ctcggagaag ggagagtatc
    24781 tcctcacatt tgacgacatt gctgaacata gccaggggtt attagcgggg gtcattcctc
    24841 gtgtccgtga tggccagacg attcttcacc atcttcagca gtatcgaggt gttttcggtc
    24901 agcgggctta tcttttggga gagattcttt tggggcctaa cgatcgccgg cagctagagt
    24961 ggttgcagca tttatcccat cgttcgcgtc ttcccctggt ggcggcggga aacgtctttt
    25021 accatcatcc gtctcgtgcg gcactctgtg acatattaac ggctatccgt ctggggacaa
    25081 cggtggatgc ccttggtgag catcgctttg ctaatgccca acggtatctt aagtcctttg
    25141 aagagatcaa cgctctctgg tatcgttgtc cggaagctgt agcacggacg cgagaaatcg
    25201 ccgatctctg tcgtttttca ctagatgatt tgcgatatga gtatccccaa gagctttcac
    25261 cgccgggaga aacaccgttc ggatatttgc aacgtcttgt ctgggaaggg gccaaaaaac
    25321 gataccccaa aggaattcca gaaaaggtgt cccggcttct cgaacacgag ctcaagctaa
    25381 tccacgagct gtcctatgaa ccctattttc taacggtatg ggaccttgtt cggtttgccc
    25441 gaagtcgcgg gattctttgc cagggcaggg ggtcggcggc caactcggca gtgtgttatt
    25501 gcctggggat tacggctgtc gatccggaac ggatggacct tctttttgag cgatttgtca
    25561 gtcgtgaaag aaacgaagcc cctgacatcg atgtggattt tgagcaccag cgccgcgagg
    25621 agatcatcca gtacatttat caaaaatatg gacgcgaacg agccggaatg acagccgaag
    25681 tcatcaccta ccgtccacgc tctgccgtgc gcgatgtggg aaaggcgctc ggattttctc
    25741 ttcaagaggt ggatcggatt gccaagcaga tcgaacgatt tcatgaggaa ccagaccttc
    25801 ttgagcgttg ccgagaggct ggaattgacg tagatcagct agccacccag catctagtgc
    25861 aatgggctac aactatcacc ggatttccca ggcatctttc ccaacacacg ggcggaatgg
    25921 ttattaccca ggggcctctt tgtgagttgg tccccattga aaatggggca atgcccgatc
    25981 gaaccgtcat tcaatggaat aaggacgact tggatgaatt gggcattcta aaagtcgatt
    26041 gtctctgcct gggcatgctc acagcgattc gtcgtacttt cgatactgtt ttcaaacatt
    26101 acggccgcaa gctgagcctg gcttcgattc cggagggtga tcccggtgtt tatgccatga
    26161 tccgaagggc cgacaccatg ggggtctttc agattgagag ccgtgctcaa atgaacatgc
    26221 tccccagact tcaaccccgg tgtttttatg acctcgtgat tgaggtggct attgttcgtc
    26281 ctggaccaat ccaggggaat atggtccatc cctacctccg ccggcgaaat ggtgaggaac
    26341 ccgtcacgta tcccaacgag gttattcgcc aggtcttggg aaaaaccctt ggtgtgcccc
    26401 ttttccaaga gcaagcaatg cgattggcca tcgtggccgc tggatttacc cctggcgagg
    26461 ccgatcagct tcgtcgagcc atgggagcgt ggagacgccc tggcttgatc gacgcttttc
    26521 gacgcaaact catcaacggc atgttggcca atggatttac gcccgaatac gccgaggcca
    26581 tctttcggca aatccgcggc tttggtgatt atggttttcc ggaatcccat gccgccagtt
    26641 tcgctttgct cgtctatgtt tcagcctggt tgaaatatca ttaccaggcg gcctttaccg
    26701 taggccttct gaatagccaa cccatgggat tttatgcgcc agctcagctc atcactgatg
    26761 ccaaacggca tggggtggaa attcggcccg tcgatatcaa ctacagttat tgggaaactg
    26821 acctagaggg cgcaactgct ttccctgttg taagttcaga gcgtgacagc tcttggggca
    26881 gatgccctga aaatgttgag tcgttaagcg aagacggagg atcattgttc ccattttcag
    26941 aggacgaaaa cagtcaatgg cattcctcgc ctgttcccga tgatatcctt cgctctggta
    27001 gtaagcattc gtttcccgag tcctctcttt taccagccaa ggccattcgc cttgggtttc
    27061 acatggttca tggattacga gaatcggatg tcgaaaaaat cattgaagcc cggaaggatg
    27121 gccctttcac aagtattgcc gatgtaatga gacgaactgg tctaagtcaa gccacaatca
    27181 ttcgtctggc ccgcgccgac gcttttggct cgctgggttt gcggcgacgg gaagccctct
    27241 gggaagcccg tgccctttca accgccacac ctcctccttt gtttcaagat aggacctcct
    27301 cggaccagag tttggccggg ggtatttcct cgtcggcaga tacccgggag caaagtcatt
    27361 ctccgccttc catgaacctg gcggaggaag ttctggcgga ttatgccatg atcgggttgt
    27421 ctctaaaggc ccatcccctt cgacttcttc gaaaagagct aaatcaactc caggttgtcc
    27481 ccgccgagcg attgctggaa attcctgacg gccatccagt tcgcgtggcg ggaatggttc
    27541 tagtgagaca gcggccagct acggcccatg ggatcacttt tatgaccatc gaagatgaga
    27601 ccggcattgt gaatcttgtc gtccgcccag atgtgtggca acgctggcga tcggcggccc
    27661 ttaatgccac acttcttttt gcagtgggca gacttcaaaa acaaaacggc gtcattcatg
    27721 tggtcgctat ccgattagag gacctttctg caaaactggc cggtctccag gcaagatcgc
    27781 gagatttttg ttgacaccca ctttttgggc acgaaaaaac cggtattcga aatttccgtc
    27841 ccgaataccg gtttgctcgg cagaatcatt taattccgta acggatagct atccgatctg
    27901 attacggaag cctttcgagg attgcctggg cacgggcctt gatcgccgga tccgttgcct
    27961 tctgcacgac ttgtgaaagg gcctcccgca acccgggaac gttcttttgc agtttctcgg
    28021 cgatcgtcac aattgtttcg caggcagctg cccgcatccc tggtcgatcg atctcggcca
    28081 ctgcgagcgc caacccttcc gccgagggat tccgccgcaa aacatccatg accaagcgac
    28141 gttcatccgg ccggtcagaa acttgcaggg cattacggca catggccaac cgttctgcgt
    28201 caggcacatt gaactgccga gcgacccgaa tgtaaccccg gatcaaacga atccggtact
    28261 ggggcgccgc atttttctga atgaggtccc aaagaacggg tgcaacatcc ggtgacagcc
    28321 agttacccaa aatagctgtt gccgcatctt gcagactgga atcctggctc tgagcggccc
    28381 ctgccacggc agcaagtgcc gtcggcccac cgaggctccc gagaacctcc agcaagtccc
    28441 gccgcgtatt acctggggcc ttatccatgt gttgaatcag catcttggcg atagcatctc
    28501 gatcccgtac ccgctggctc gccgcctgaa gagcatcctt cacggcagca acgtcgtcag
    28561 cggatggcga cgttatcaag cgttccacca gcatcgcgac gtcttcatct tggccgaggt
    28621 ttcccaacgt gcggatcgcc gccaggcgga tactcggatc agcgctggca gccaactggc
    28681 ggacaacggg agaggccgcc aacgtccgac gctgcccgac aacttccaag agcacaagtt
    28741 ttccctttga gtcttccttg gcaagggagt cggcaagggc ctccccaact cccgggcctt
    28801 gcaaagacac gagggccgcc ttagcttgct cggctactac ctcatccgag gactttgcca
    28861 cttcgagtaa aagcgccaca tcggacttgt caccgcattt cgcgagactt cgtatcgctg
    28921 ccaacttcat tgcgccgctt gcctcggcca cagccttgcg aatcgcaggc agcgcttcct
    28981 ggacaccggc atctgccagg gcttcaacaa ttttggcctg cctatcttcg gaaaccacac
    29041 cgagatgttt gaccagttct tccgttgcag cggcagactt catttctctt gccacacgga
    29101 gggcaaggtc gaatacgcca ctgtcgtccg tctccaggag ctctttcaat aaggtcaccc
    29161 cgttgtcacc ccttgccatc acggcacccc gcatcgccgc cagccgaacg ggtttgggaa
    29221 catccgcctt cctgaccgca tcgaagatgt cgatggcaag ttccgtgttt ccttctttct
    29281 tgacgatttc cgcgcagatt aaaagggcat ccccgacggc gcgtcgtgtt gtggcctttt
    29341 cactttgaag agactttttc agaatgtctg cggcttctac gtttgcaatt cgccctaatg
    29401 ccgatgccgc ggcatcgacc acggcaggct cgtcatgatt taacaacggt gcaatcgcca
    29461 caactgcctt ggtgtcacgc cgtacaccga tcgagtttaa gattcctaga cgcaccccgc
    29521 cctcggctcg cgaaagggct tcccgaagcg cgtcgtcaac caccgccgcg gggatcggct
    29581 caagggcata tcgggcatag tgggagaact tttcatccaa aagcatctta gccaggacgg
    29641 gaacggcctc ggccgtgccg aaaaccgcca gacgtttgca ggccaccgcc ttctcaaagt
    29701 ccgaggattc tttggactcc aagatggcga ccagtttcgc actgtcggtg agggttgaaa
    29761 catcctccgc gacgcataac cgccccgcga tgatcgttat cagacagaag atggcagcga
    29821 gtattcgagt catgattgtc ctcctatcag cgatcggttg atgatactcg taagggagtg
    29881 ttaaatatgc cacggctccc gcaaggcccg ggaacgcatc cggtttgctt catcgtcccc
    29941 gatgaactct tccttcacgg gatcaaagcg aagcttgcgg cccaattgcc aggcaatgta
    30001 ggcggcgtgg caagcgatgt gcgactgtgc cgcgacgtca gcattggcgg ccggcatagc
    30061 ccggcttttg acgcaattga gaaagtcgcg aatgtgcgtg gaaggatctg tgcccgccat
    30121 ggtaaagacc ggcatttctg agcggagcga ttccggataa accgcaacct ggccactgtc
    30181 gccggtctcg atccagccct cgtcaccttc aaatcgcaca ctacacgtgc ccaaacccaa
    30241 ccaaccggta tcgcgcatga tcaacttcac accgttggcg taagttgcgt ggacatgatt
    30301 gccctgtggt tcaaactcaa tgggtgccgt gttatcggct ttgttggccc attggcacag
    30361 atccacggtg tgcgatcccc attccagaat cccgccgccg tggaaatcaa aatgcccccg
    30421 ccatccacca ttgacatagc gttgattgaa aggccgccaa ggacaaggtc ccagccacaa
    30481 atcccagtcg cactcgtctt tggggggttc cggttccgca ggtagccatt cgtgcgtggt
    30541 tcttggatca agcgtattcg catggaccgt atgcagcttg cccaacttcc cactgtatac
    30601 gagattgatt gcgaatatga agttaccgat gtttcgccgc tgggttcctg cttgatacac
    30661 ccgcccgtag cgccggatcg tttccgccag ggcgcgactt tcggcgatgg tcatggagca
    30721 tggcttttcg caataaatgt cctttccagc tttggcagcg tagatggatg ccatggtgtg
    30781 ccatcgatcg ccggtagcga tcaatacggc gtcgatgtca tcccgcagca agagatcaaa
    30841 catgttgcga taggtcttgc agtcggagtt ctgatgatgc cggtcgacca tctgtttgac
    30901 ggcttcccgc cgtgccgctt gggcgtcggc cacggctacg aattgcacgt cgggattctt
    30961 caggaacgcg gccaggtctg tccgaccacg cggcccaatc cccaggccgc ccatgacaat
    31021 ccgctcactg ggcgctacga agccatttaa tcccaaggcc tttcccggga tgagggaagg
    31081 aaccgctaag gccgacagcg caccgatgct cgtccccaga aagcgacggc gacatatctt
    31141 tgtcacgtgt ctcatcgatt aacctccttt tacttggtgg ggttgctttc agaaaaacag
    31201 ggttcccttc gttcagtcca agccggcaaa gtcactgggg cgcagaccct tcttataacc
    31261 gttgaagcca aaatagtttg gctcgaattg gcccacgatc tccacgttgg ccttcttggg
    31321 aatcagatcc tccattccga ggcaccaata aacggcatta accaccaatc gccggagtcc
    31381 ctcgctcttg aaatcgactg ccgctcccat ggtggttgta aagacgcgcc cggccggacc
    31441 attctcccat gtgtaggtct tgatccacgc caccggcatc ataggatcgt tcttcgggcc
    31501 ctcaaccggc ttatcttgcg gcgacatccc ttcgagaacg gcaccaagga ccaaaggctg
    31561 ggaatccccc ggcaaaggaa ggcgaacgcg ataaacgtcg gttggtcccc aaatgtcgtc
    31621 acagccacga acgatgggat ggtctttcat ctcgggcgca atgacgccgc gggtgctttc
    31681 cttgttgtgg gcaccatgat gggcaatcca tgtctccccg aggacgatgc gaccgaagcc
    31741 gccttcccaa ccgggctctt tacaatccca gctcatatgc gcatatgttt ttccagccgg
    31801 tatggcaaag gcgtgagtcg ctgtgcgaaa tcccataacc ggccgacctt ttttcaggta
    31861 attcacgatg tggctcattt gctcgtcggg caaatcccga aagcgggtcg caataatcat
    31921 caagtcggcg ttttcaaggg cctcaagtcc agggatattg tcccgaacgt ttggggcaat
    31981 ttcaccagtg gcggggtcga tggcaaacaa cactgtgcac gtaaacccat gccactttgc
    32041 caagattttg cccaacatgg ggagggcctc ttccgagcga tactcttcgt cgcccgatat
    32101 cagaacgata tgcttgccga gaccaggccc ttgctgcccc cgataaacaa cccagggacc
    32161 gtcttcgtcg gcaaaggcgt aaaatgacca cccactggcc aatagcaagg caatagccag
    32221 tccgaaaaca cccctaaagg aggccatttg gtgcacacgc atggttgttt tctccgtttt
    32281 cgtcacccaa aacctattca aatcagttct tagcaaatag tgtaaacacc acttacccat
    32341 actcaaatgg tgatggctaa gcacagcgca cggacatttc cttcaattgc cgtcattacg
    32401 ggatatgaag tacctcccgt ccgcggccct gctgaatgag aggcacgtag tcttctagat
    32461 aggcgagcgt ttgcgggtcc gtcaaatcga cggttgctcg ccgttccgcc gacagatagg
    32521 ctgccatcgt caatttcaca atttctctac cgaagtccca gtccatcagg gccggacgac
    32581 ctgtcagcaa ggactccaga gcgtcgatgt tttcgtccac gtaaccatac agatcgggtt
    32641 cgttaggttg gacggtgacc aacccgcgcg ttgcttgctg tttttccaag gcggcttcgg
    32701 catcggcaac agcggcggcc gccacgtcac ccacgaagac ctccagtggc gaacggagcg
    32761 tattgaactc gaaggcataa ccgggtccaa tcccgtcgat catgacccgg agcccttgct
    32821 tatcgtacat ccacgaaact gtgaactgtg ccttgctgat gcaattggtt tcaggattca
    32881 agaaggtgat caaccccgtg gcgaagtcgt ccgcaggagt cttggagtag tcaacgccga
    32941 atcgctgtcg aagtgcttca cggtattgag ggcggcccca ttttaagagc gacagatcgg
    33001 cttgaacggc gattggccgg agaaaaccga gcggtttgcc cggcggcgtc agcaaatacc
    33061 aagctacggc gacgctatgg caccccatgt cggtcatcac gccaccaccc tgttgaacag
    33121 ggtcccagaa ccacgcactg tgcggtcccg cgtgttcctc cgacgccctg actagtgtca
    33181 gagggcccat ggtctttacc acgggttcaa gttgcgcccg ggcctgctgg accgccttca
    33241 tgtggagttg gttctcaaaa tagatgagag gcactccaaa tggccgtaac cgatcaatca
    33301 tctgccgggc ttcccgaaga ttcctggcaa gtggcttatc acagatcacg gcccgcagct
    33361 tagccccctc gtcgagggct ttcccaatct cttccacaat tggtacacgt gcgaagttgg
    33421 gggcgtaaat ggccaccaca tccacgtgat gggccatctc tcggacggag gggaaaattc
    33481 ggccctcccc caaccttttc gcttgaacaa attgcgacag ggcttcaggc gggcggcgcg
    33541 agactagacc ggctacctca atccctcgaa cttgttccag cgcacgcagt tgaaacttcg
    33601 cgtaaaaccc accacccact atgccgtaac ggattgtctc catctcacac ctccgttgct
    33661 ggattctcga gtaccgttcg cgcgacctga accgatgtcg aaatcagaat ccgcctcacc
    33721 ggcgattatg tgctgtcatc atcgtacggt ctcttagaac cctccgcaac ccaccgtgtg
    33781 gtaagtcaaa ggaaatataa cgcgggccac gtgttgcgcc agtggcactc caaatttctt
    33841 tcgttgcgaa aaacacgcgg ggcctcgaga tgccgtaatg cttggcgcat gtctcgtttg
    33901 tcctcctcgg gacagtcaaa ttgccaagcg atccattcgt cctcggccag gctgtacggt
    33961 gtcaggggat cgcgtagaac agcgaagcga cttgccggaa ctgatagtag agccgacgga
    34021 gagtgtcgta atccaactct tttatgcgga tatcaatgtc ccacagggac tgtttagcga
    34081 aagcaaccac tgggaacggt tttcaaacgt aaccaatgtt acgctgaggc cgcttgcttg
    34141 atcttgccaa acctgcacat atcgggtgcg attgaggtcc agtcgttcca cgttgtgtgt
    34201 acagggccac gtggcaagca ctgtcgtggg aggccgattg tcataaagga acgaaaaagg
    34261 gcacaccgcc accgtctttt agatattggc ccacccaggt tctcgcatct gcccgctcgt
    34321 cttctcgtgg aagcacggcg aatacggagt ttgccgtagc caaaaccgtt gccaaaccga
    34381 tagccgcaag actcagcggg cgacgtattc gcatactttc accccacaga cagcgccaag
    34441 ctaattgcac cattggcggc ggttatcgtg ataatggttg ggatacaaat ggcttgctac
    34501 tgacaggggg agctatggtt tcttctgttc caagatttca atgtccttga acatgaccag
    34561 catgtcgttg ccaccgtgaa gctgcaaggc gaaatggccc tcacgacgac cgggatcatt
    34621 ttttacctcg gacattttta tcccgttaag ccacacggtg atgtcgccac cttgggccga
    34681 aaccaccatt tcattccact cttggaattt gaatgccttt ttcaccagtt ccgggtcggg
    34741 ccgcacaacc cacgcccggc cgttcgtttc atacagcccg ccggtattgg catcgggtgc
    34801 aatttctgcc tggaaacccg atatgccgct gtgaccttct tccacacatc gaaaatagaa
    34861 accgctattt ccctgcagcg ccttgaattt aagccgcacg acgaaatcac caaaggcgcc
    34921 atcgcttacc aaatggccaa actctttttc tgcggcattc ttcctcccga cgatgatgcc
    34981 gtcttcgatg gtccactgac caacaccgat gatatgccag ccctgaaaag tcttcccgtt
    35041 gaaaagtggt ttccattgac cgctgggagg ctgagccgcc aggactagac ttgcaactga
    35101 cgtcaagccg acaacggcca gaaggatcaa atacttgcag ctacgcatga tagacctccc
    35161 tagtacaaaa gatggttgga acacgtgctg atcttcgccg ggaaaacaaa caaccggcca
    35221 accggacgcc agcatagtcc cgcaggcttg acgaatcaag actcgccacg cgactgaagt
    35281 ttacccgctg tttggagcca cagctcccgt ttcggattaa gctggcgagc gcggttggcc
    35341 gttggtccgt caatgacgcc tggtaactct caatgtagcg ctgggcgtta agagtctaat
    35401 cccaaaaatc cccctctccc gtcgggagag ggttggggtg agggcaagcg atcgtcggag
    35461 tcgatcgaca aaactggcag cgattcgagc caccctcacc gccgatcgct ctcccgccga
    35521 acgggcgtgg gaagatcatg gcgtaggctg taagcctgag taccggtcaa ctgccggcct
    35581 tcgtcaacat ttgttccact tcatccctag tggggaagga agtttgtgcc cctgggcggg
    35641 tcacggaaat ggccgccgca caggtggccc aacgaattgc ctcttcccac ggttgattct
    35701 ccgccagtgc cgcggcgaga gccccgttga aagcgtcacc ggcgccggtg gtgtccacgg
    35761 cattgacgcg gaaagcggga tatgctgcgg ttcgctccgc atccaccaca agacagcctt
    35821 gacttctcag tgtgacgatc accttgttga cgcccttctg ccttaggaca taggccgccc
    35881 gttgtgccga atcgatgtcg gagacctcaa ctcctgccaa tcgggagacc tcagactggt
    35941 tgggagtaat aatgtcaacg gatttaagca tctcaggggg agggctatcc aaggccgggg
    36001 cgggattgag gatcgtgatc aatccggctt ccttagctcg cttcaacccg gcaatcacag
    36061 tctccaaagg gatctccagg caggtcaaaa aaactttggc tgagcgaaaa actgtctccg
    36121 ggaccgccgc aacatcggaa ggcgacaaga gggcatttgc cccggaagcc acggcaatca
    36181 tgttttcggc gtgctgatcg acgataataa gtgcgacacc cgatgggctt tggggttttg
    36241 tgaccacgta ctcaaggtcg atcttctctt gaccgaggtg ggcgagactc tcccgcccaa
    36301 atccgtcgtc ccctacagcc gcgattaagg taacgacgga gcgtcccaca cgtgccgcgg
    36361 cgaccgcctg attagcccct tttccgccct ggctttgata gaacgttccc ccaatgaccg
    36421 tgctccctgg ccgagggagc tccggaacct tcacgaccaa atcggtgtta atggagccaa
    36481 gaacaatcac tcgagcgtca ttcatcgttg tcatgtatgg ctaaaaagct tttttcgaac
    36541 gaacattgtc gcagacccaa atcccctcgg atctcccgtc tgccgcgatt gccgcgtcgg
    36601 agatatcact tgccgatgca gaaccgcgaa aaaatgcggt ctaacacatc ctcggtgtaa
    36661 accgtcccca agatgatctc gaattcatca atcgcggcac gcaaggcact ggcaatagtt
    36721 tcttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttcg atcaatgctc
    36841 gcgccttggc ctgggctgcc tgatccacta ttgagttcga gccttgaaag gaatgatacc
    36901 ttgcagcatt ttcggtagat tctactccca tgggcatctg tgacgagaca ctttgcacga
    36961 gctgccacaa tccggcggta tcgacaagtt ggaacttgac cccgccgtgt tcgcacaccg
    37021 ctgttaagta atcgcgcgtt gtgcccggaa cgggcgacac gatagcccgc gtgccaccta
    37081 atagggcatt aaacaaggtg ctctttccaa cattggggcg gcccaggagg acgaccatag
    37141 gtagctcatc ggctgtccgt cgtttgaaag cacgttcgat gatttcggtg accgactgcc
    37201 gggcttgtga cagacgttgc caaagcacat cccgcgacaa aaagggcagg tcttcttcca
    37261 cgaagtcgaa gcctaactca agttcgacga gcaggtctaa cagtccttca cgcagtttct
    37321 gaagtggcgt gcccaatcct cccgcgagtt gatcaagcgc gtgacgcagt tcctcagggt
    37381 caaccgcgtc gatcacaccc agcacggcct cggcctgcgt caggtccaat cgcccggaaa
    37441 ggaaggcccg caaggtaaac tcgccaggtt ctgcgggacg cacctgcaac tctgcaagaa
    37501 cggcctccgc gagaggatga caacaccaca tatgaatctc ggcgacggcc tggccggtgt
    37561 agcttcggcc cggtggccaa tagtagagct cacacggcac ggttccatga agttggcgca
    37621 aacgcaaaga cgcggcccga ataaccggct ctgcgggcac caccgggagg tcattatcac
    37681 aaatcgtgat gacccggtgg acggcttgcc aggtctcggg acctgaaatt cttatgatca
    37741 gccgtgcacc gaatcccttc ggggttgcaa tagctgcaat cgtctcctcg ggatcgcgga
    37801 tcatagccga ggctcatttg gtgattgtcg agcgctgccg gcgtgctagc ttttccgcct
    37861 gattcttgtc ggctgcctta agcaactgga ccagccattg tggcaaccgg atcttgacgc
    37921 ggctccattg cttacgttgc tttcgttgct cggcgccacg atccgggcgc ttttgcgtcg
    37981 ttttgaggag gatttgccgc tctgccattc cccaaaggct ggaggcaatg aaatagatac
    38041 agaggccact gggtacctta tagaaaagaa tccccataaa gatcatcatg aaattcatga
    38101 ttttctgctg catagcagct tgttcatcgg tgggctgggg catcatcttt ttctgctgcc
    38161 aaagaaaaat ggcaatagtc aagattggta agatgttgaa atacggccca agcgcaaaca
    38221 tcccctgacc acgattgaac cattccggcc ataggaaact ccaatcgaac agcatatcgg
    38281 gcgctgctaa attcgagcac cagcgaatcg actccgaaat caggggtgcc tgacggagtt
    38341 cgacgtcaat ggccagtgcc ttatacagtc caatgaaaat aggaagctgg ataaaaagga
    38401 ccaggcaccc ggagaacggg ttgtagttgt gttgtcggaa aagctcttgt gtcgctcggt
    38461 tccgttcctg aaggtccttg t
//
LOCUS       DAOSUT010000015        37674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_764_length_37674_cov_9.535182, whole genome shotgun sequence.
ACCESSION   DAOSUT010000015 DAOSUT010000000
VERSION     DAOSUT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 37674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37674
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_37674_cov_9.535182"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1075
                     /locus_tag="PLD05_02430"
     CDS             176..1075
                     /locus_tag="PLD05_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HOP76316.1"
                     /translation="MMSQQTPSAVRLQNAFLRLQDKSQGLVVKEPATTGLSANAHDTE
                     ETCAEAATSFRPALEVDQFAPPPLVSQLLESISPILAGCLRQIRQRLPKSERVVGLIS
                     PERGHGCTTTAILLASRLSRERATALVIDASPDRALADSLSLEIESGWEQAMLRRRPL
                     REVIIRSNSDGFDILPGQPGALASKVTGVQPTDWPDLLKAYDWILIDFGSSFARTAYS
                     PTGTDLSEDDGEESNAVVRSGTGTSVPFVSLCSSFVMVQRCDSGTARFSLPAGAAEIL
                     GVIETFVQPTAESQVLVNPKEAA"
     gene            1118..1930
                     /locus_tag="PLD05_02435"
     CDS             1118..1930
                     /locus_tag="PLD05_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP76317.1"
                     /translation="MYGNYWHFREPPFRNHFRRAFFFASPSHQASLLKLRYAIEQREE
                     AALLVGEAGTGKSFLTHVLTESTNESVAPVVRTCCLFDAPEELMTHLAAEFVGRNFNG
                     SGYAALQELEAFLGRNLEEGHHAVLILDDSHLLQSIRSWEVLRSLMTLKIQGETPWTL
                     IVLAQPLFLPLLDRLPQLEELFALKAFLRAWTPEETSQYIKHCLTTAGCDEDVFSPDA
                     VEVVARFSSGIPRRINRLCDLSLLVAFAEKRYQVTAAHVEAVHEELMTVPCQ"
     gene            2003..3820
                     /locus_tag="PLD05_02440"
     CDS             2003..3820
                     /locus_tag="PLD05_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOP76318.1"
                     /translation="MRSHLSRGLRLQVGRPQRAIWCGVCAALVAAGALLGTSPWTPMT
                     WDEGDAIRRAESIRDWFSHAFRMEASGPPALSREGITRGWPFTVRREGHPALYGAIIA
                     LGRSLSPGFLDPLTRYRFGPIVLFAAAMGAVTYRVARIAGVSPAVAAFLCILLQPRLF
                     AHAHFASFDGPLTAWWMLAWACSPFFSTMRTSHKRTSRSDLARIFLWGLILGATMSSK
                     ATGWLVVVPFLFWAIGQRQWSVLFRTIVGILIGLGTFVVLNPPLWYDPISGVLRFFQL
                     NLSRHERPDLNITTFFLGELYNLDYPLPWYNTLFWTAIVVPIPILALFIVGLVYWVRT
                     SRRSILTLVVLHWLTLIVVRAIPGTPPHDGIRLFLPAFPFLGILAAFGFRFVTSAPII
                     LLRRWAMGFLPLPRRQRSIRAAQPLAETRSFSWRRNFLHTAHSVLQTVMWLLVLTPLI
                     NLICFGPQWLSYYNGLIGGLPGATAAGMEPTYYWDGLDREVFLWLEIHCLESEKVYYS
                     ACPWTNLRLLQEWGMLKRAFATSPADAQWYVVQNRPGAWFPEDKWLFTHGEAAFTKYP
                     GRGPMCPWVGRVPVVKIFPIREYFRACQAVHADRAMSDQ"
     gene            complement(3923..5029)
                     /locus_tag="PLD05_02445"
     CDS             complement(3923..5029)
                     /locus_tag="PLD05_02445"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOP76319.1"
                     /translation="MAKTVRVLIVDDSIVMRQMIKDFLVTAPGIQVVGMASNGREALR
                     LADELAPDVITLDIEMPGMDGLSTLDTLLAKRPIAVIMVSSLTQAGAAITLDALERGA
                     IDYLTKPAHKSEAATVFGQELIRKIRFAATSDVNRIVRLRKQRAQSLRMAEPVVKSPT
                     ESLEGPPTLSRACIAIGISTGGPPALTSLFRELKPPLPPILVVQHMPPQFTKPFAWRL
                     NSVSRLTVKEAESGDLVKPNHAYVAPGGFHMAVQGRSGSARIRIFDGEPVSGHKPSVD
                     VMMSSVAQVFGRRVLGVIMTGMGRDGANGCRAIRAAGGYVLGQDEATSDVYGMNKVAF
                     VEGNVDRQFPLQEGARLIMRYVEEKFLAQPSEIC"
     gene            complement(5310..6578)
                     /gene="hemA"
                     /locus_tag="PLD05_02450"
     CDS             complement(5310..6578)
                     /gene="hemA"
                     /locus_tag="PLD05_02450"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOP76320.1"
                     /translation="MEICVVGCSHQTAPLAIREKLAFSPEQTRAALQLWEKRFPWVEA
                     VLLSTCNRVEIYLASEMATIPPATDVVDFLGECHGLTRQDFADLCFALCTPQSLEHLF
                     RVAAGLESMVLGEPQILFQVKQAYQWATEMGTVGPLLHIVFQGAFRAARRVASETEIQ
                     KRRVSIPSIAVSDFGRAVFERFEDKLVLVIGAGKMAEETIRYLRAEGAQRIVIINRHL
                     ERAHQLAERCQGEVRPWEELPVVLREADVVVSTTGAEQPIVTREMLSRVIAARLERPL
                     FVLDLAVPRDFEPAIATMPGVYLYCLDDLMDVCQKNREARDKELPRADEIVAQEVGSV
                     LANLRQRTVGPVIQQLRKDWESVKEQELRRLFNKLSDLDPAIQAEIRLAFDRLINKLL
                     HPPLSSLREYSQSGAHQEMIEAIRKLFRHS"
     gene            complement(6691..7731)
                     /locus_tag="PLD05_02455"
     CDS             complement(6691..7731)
                     /locus_tag="PLD05_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76321.1"
                     /translation="MGEMIAIMVCAYGLAFLMELGRARGWHVSTRLIGGLLVLGLIVH
                     TGWLIYRIALFQEQVELGGHVLSIRQDWYYGIAWGLALICLFWMIIRPATPFGVFFLP
                     LILLLIVLGVVWGDTVPFARGAASRSWGIIHGFSILTAILAVCVALLSGVMYLGQVYR
                     LKRRAIATSRFRLPSLEWLHNVTKNALFVAVAMLAVGIFSGLWLNVVQYSTLQGHLPW
                     NDPVVLSTWILFFWLGGSLLLGFVYRPLREGRRVVYLTIVSFVFMLLVLGMTLFGATR
                     HGQQRELPDGGLPVKSTQWQGMDPWKYLGKKRAFCLKSSAVSLMPEPGMSRKSGFLNC
                     KKTQGYSATGTQ"
     gene            complement(7834..8934)
                     /locus_tag="PLD05_02460"
     CDS             complement(7834..8934)
                     /locus_tag="PLD05_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP76322.1"
                     /translation="MLTPDDSPPIPELIGSSPAMREVYRLTRRVANSDASVLILGETG
                     TGKELIAKAIHQLSPRRSGPFVRVNCGALPEGLLESELFGHVRGAFTGAIENRTGRFE
                     AAHTGTIFLDEIDSTTFKLQVKLLRVLQQHEFERVGDTRTIHVDVRVIAASNRDLLEE
                     VEKGRFREDLYYRLNVVTIYLPPLRERREDIPELVGHFLRLYNEKNRRYVPFVHPKAM
                     EALKNYDWPGNVRELQNYVERAVILAPGDEVTCDLLPDAVLGRRMPRMGYRRKDLDEL
                     ARDLVAAALAQFPPDHKQLYQEIVGRVEKELIHQVLARCNQVQIKAAAQLGLNRNTLR
                     KKLKEWGWVDATEAEDDGSASRDLPLPSEETF"
     gene            complement(9163..11469)
                     /locus_tag="PLD05_02465"
     CDS             complement(9163..11469)
                     /locus_tag="PLD05_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76323.1"
                     /translation="MRSVRDTKVVIAAVVFVFLSAKVLPAEPAKRGPAADGAPAQTSS
                     GSDPMQVAAKEDPAVAAILATNPLTPRERIRAADVLLSLNRPDLAKQMLANVLQSPLD
                     PPTLLALDREFGSATFFRFASHPELDPEGTNLAELVFDAVRNTRCDAERLAMLVNYLC
                     EAPDNCWYRAAKHLIKAESAAVKPLCDALIDPAWRASWPRVYYVLLHFRPASDEALLS
                     LLFTLPEEKQAGIIGGIAALGVTRATPFLLCLAMGPEGARGEQQLAERAIEQLGESLP
                     TPRQAARLLRAEVEAILIESVPLDHEVLTLLRWDASAKQIVAKRVSFEMWRRERAAYL
                     AQHAARLTPDDGELRDLAVCLQLEKEGYLARVEWDSAEIKRSNLSAATAPNIRAMADT
                     VKQILEPGKPTAEDVNRWLNQCLTREWYHASWAVACWMKEYADAGVLVGRDVPALVRC
                     LDAPDPELRFLATDVALSLAAGGSFRGASRVTKELAYFASSEGIAGVLVADPVLRRAA
                     QMTSYLKTIGYARVDTAADGGTLLEMARQSPDYEVILVSAAIQRPGLSLTLQRLRAEP
                     RLARVPVIVYADTDWLPLADSTVRTIPVALSVATPRDPQDLAGLIARAKMLPGVQRVS
                     AQQRLEFAQKAMAWLRVLAEHPPEGISQMEIEEVALRAINVPQLSEVSLEILGQMATP
                     KAQSALVEAASRSDWPIAQRVKALGAFRKAVEKRGVQLTTQQILRQYERYNQSAQSPE
                     EVRKVLGLILDCIEAPTQVRSVGTQAKE"
     gene            complement(11704..13095)
                     /gene="argH"
                     /locus_tag="PLD05_02470"
     CDS             complement(11704..13095)
                     /gene="argH"
                     /locus_tag="PLD05_02470"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOP76324.1"
                     /translation="MTEKPWGGVFREATDQRVELFTESISFDRRLYRQDIAGSIAHAR
                     MLAAVGLLTRDEAAQIVKALEEIREEIDAGNFEFRVGWEDIHMHIERVLIERLGDVGR
                     KLHTARSRNDQVATDFRLWVRDAIDTILPLVRKLQAAFVNRCEADFDCVLPGYTHLRR
                     AQPVLAPHYWLAYCEKFERDYQRLLDCRKRVNICPLGSAALAGTSLPIDRHMTARELQ
                     FDEPSPNSIDATSDRDFAVEFVFDLALLAVHLSHWAEEWIVWSSEEFGFLKLPEGFCT
                     GSSIMPQKINPDVLELIRGKTARVLGHLQQLFVLLKGLPLAYHRDLQEDKPGVFDAYD
                     TVVGCLEVAIPLVELTELNRSRIEESLERGHLDATTLMEYLISRGIPQRTAHSLVGRI
                     VREAMDRQCRLADLPLEVFRMADPSLDANVYSVLGVKNAVQAFRSYGSTCPEEVRKQV
                     ERWRERLARDLGA"
     gene            13639..>13961
                     /locus_tag="PLD05_02475"
     CDS             13639..>13961
                     /locus_tag="PLD05_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOP76325.1"
                     /translation="MPRRNDIRKILLIGSGPIVIGQACEFDYSGSQACKALREEGYEV
                     VLVNSNPATIMTDPEMADRTYIEPITWEFVAKIIERERPDALLPTVGGQTGLNVAMDL
                     YRHGIL"
     assembly_gap    13962..14061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14062..16707
                     /gene="carB"
                     /locus_tag="PLD05_02480"
     CDS             <14062..16707
                     /gene="carB"
                     /locus_tag="PLD05_02480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOP76326.1"
                     /translation="VLGWKEIELEVMRDADDNVIIICSIENFDPMGIHTGDSITVAPV
                     QTLTDKEYQRLRDAAIAVIREIGVDTGGSNIQFAIHPHNGRIVVIEMNPRVSRSSALA
                     SKATGFPIAKIAAKLAVGYRLHELANDITRKTKACFEPTIDYVVTKVPRFAFEKFPEA
                     DPTLTTQMKSVGETMAIGRTFKESLQKALRGLEVGSFGLGCDGKDLWGTPKQPSIDEI
                     RYQLSHPNPQRIWYIRYAFKAGMTVEEVNELTSIDPWFLANIKEIVDFENHLRTYRDI
                     SEVSDETLREAKRYGFSDRQLAHIWSRAEMEIREERKRRGIVATFKAVDTCAAEFEAY
                     TPYYYSTYEDEDETPPKKPGKKRIMILGGGPNRIGQGIEFDYCCCHASYALRELGMET
                     IMVNSNPETVSTDYDTSDILFFEPLTCEDILNIQDRVQADGVIVQFGGQTPLNLARAL
                     QVAGVPIIGTSVDTIEDAEDREKFAEVLRRLNLRQPPNATARTFSEAQLAIQRIGFPV
                     LVRPSFVLGGRAMEICYDMNQFEEFVKKAFDAAEGQPVLIDRFLEDAIEVDVDAIADG
                     DLAIVAGIMEHIEEAGIHSGDSACAIPPYSLPGPVVEEIRQATQLMAKALNVKGLMNV
                     QYAVKKEDGRPVVYVLEVNPRASRTVPFVSKAIGWPLAKSASLVMVGIPLREQGWTKA
                     PTPTRVSVKEAVLPFVKFYGVDIVLGPEMKSTGEVMGVSRYFSIAYAKSQIAAGTALP
                     RSGRIFISVADRNKHQIVELARRLQNLGFELLATKGTADRLEQNGITVTRLKKIKEGH
                     PNVLDYILNGDIQLIINTPSGKGARTDEGHIRASAIAAGIPCITTISAAEAAVKAMEA
                     MAQDRDFDVEAIQDRFPEYGRPPKS"
     gene            complement(17104..18363)
                     /gene="glgC"
                     /locus_tag="PLD05_02485"
     CDS             complement(17104..18363)
                     /gene="glgC"
                     /locus_tag="PLD05_02485"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOP76327.1"
                     /translation="MATVKNEQIVTVILAGGKGTRLDPLTRDRAKPAVPFGGAYRIID
                     FTMSNCLNSGLRKILILTQYKAMSLDRHINMGWQKYFCRELGEFADVVPPQQRIDENW
                     YQGTADAVYQNIYAIEKEQPEYVVVLAGDHIYKMDYRKLVDYHIGCGADVTIGVLKER
                     CETARRRFGVMEVDTNNRVHGFQEKPDNPKPIPGEPEYCYASMGIYVFPSRFLFEILC
                     QDATRRGSTHDFGRDVIPSLIEGHRVFAYPFMDENRKKNSYWRDVGTLDSYYEANMDL
                     VSVEPELNLYDELWPIRTYQPNLPPPKFVFADPHRRGQALDSIVCNGTIVSGGTVIRS
                     ILGHRVRINSYAYVEDSILFTGVEIGRHARVRRAIIDKRVRIPEGMEIGYDPAKDKAR
                     GFVVTESGVTVIAKAETSSDLWEEVKL"
     gene            complement(18958..20913)
                     /gene="asnB"
                     /locus_tag="PLD05_02490"
     CDS             complement(18958..20913)
                     /gene="asnB"
                     /locus_tag="PLD05_02490"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOP76328.1"
                     /translation="MCGIAGGVWYEPTAAISAATLQRMTEVLQHRGPDDQGFYLKEIA
                     ADGHAPACGVALGHRRLSIIDLAGGHQPMSNEDETLWIVFNGEIYNFRALRQELEAAG
                     HRFRSRSDTEVLLHLYEEKGLHFLEFVNGMFGLALWDEPKRRLILARDRLGEKPLVYY
                     ADSQRLLFASELKALMEVPGVPREIDPFAVDAYFLYQYVPHPWTIYRGIKKIPPATMA
                     IYEPKNLTLHRYWDPDFSRVENGMALEEIKEELRSRLEKAVTMRLEADVPLGAFLSGG
                     IDSTIVVGLMQKNSREKVRTFSIGFPVAEYDETHYARIAAEAFGTEHREHRVNPDAIG
                     VLPSLVWHYDEPFADSSAIPTWYVAEKTRQHVTVALTGDGGDELFCGYTRYWATAFAE
                     GIASLPRWIRWIFNERLWRWAPRTRQRSFFGRVHRFTESLPLATPQRYIDWIAIFNRA
                     RRLALYTPEFQEQLTAVPEDFFLEIYSRFASRDIVSAISLADLFSYLPCDLLVKVDIA
                     AMAHSLETRPPFLDHEFVEFAGRLSGRLKLRGRKGKHVLIAAFRDLIPPPIQKRGKMG
                     FGVPLDHWFRGPLSELTRHILLDPRTLARGYFRREALEQLLSEHNNGLWNHSHRIWAL
                     LFFELWQRKWTDGEDVADLKRCLASVV"
     gene            complement(20935..22065)
                     /locus_tag="PLD05_02495"
     CDS             complement(20935..22065)
                     /locus_tag="PLD05_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP76329.1"
                     /translation="MKTVHVIPTLDRAGAEKQMVYLATGLKARGVDVHVCVLTRDGPY
                     REDLERAGIPITLIGKRGKIDLFALRRLQRVLTEVRPDIVQTWLFAANVYGRLAARRA
                     RVPHIIASERCVDLWKKPWHFWIDRRLARVTDRIIANSSGVRDFYVQRGISPEKIEVI
                     PNGIMVPQRDTTSRQSILAELGLPQDARLIGLVGRLWPQKGVRDAIWAADLLKRIRSD
                     VHLLIIGDGPEREALIRYRNSVEIQDRVHFLGHRNDVLRIMPHFDALWNTSRYEGQSN
                     AILEAMTLKVPVVASDIPGNRDLVVNEVTGFLVPLGPHFRAGVARFTKKILEDPDLAR
                     RLGEAGYQRVLTEFTVEQMVIRHEKVYQELLAGSPARDFRIA"
     gene            complement(22238..22783)
                     /locus_tag="PLD05_02500"
     CDS             complement(22238..22783)
                     /locus_tag="PLD05_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOP76330.1"
                     /translation="MNVLIDEATLQAGLDRLAAEIEKRYRGKPLTLVGVLLGSIVFLA
                     DLMRRLTIPYGVEMISSRQVTTEGNIQGPLFIQADLLAPEVAGRHVLIIDDIYDTGET
                     LWELIPQFDDFKVASVHAAVLLKKKNRQRVPLGPTLVGFEIPDVYVVGYGMDYRGYYR
                     NLPYIASLSPEEMKNLAQNGG"
     gene            23079..24740
                     /locus_tag="PLD05_02505"
     CDS             23079..24740
                     /locus_tag="PLD05_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76331.1"
                     /translation="MVRVAEQLLFYSCYWRRRFPLFVWAASLIWAWASAFSPIKAQLT
                     PEHPAVRQAVERALAYLASREAEEGYLQIGARALRGLAFLKAGHAHDHPVIVKSAEEI
                     IKQIPPSGLPKDFRMSNWDNYSTGLALIFLCNYDPQKFRQEIQLILQYSFFRQKNHGG
                     WGYPSNETGDTSQTQYQVLGLWEAIRAGFAVPSEVLDKVALWLVWTQDPSGGYGYQGN
                     FPPTPTLIPQSDVRIGMAVAGTGSAYVLASLLPGFRGTLERSDLPPALEKIAQARQAG
                     RGRLTIDPKLLRAAIQRGNAYIAKHFQVAPDRYRYYYIYTLERYFTFREAVEGPEPGL
                     FDWYTACAEYLLKNQQQDGSWMHQCGKVVDTSFAVLFLVRSTKQSMEGVRDYGPGVLV
                     GSRGLPKQTDNVIVKNGQVVALPLRTSAEDLAAVLNSPEMSDLVSAIEAVEAMPPEEI
                     RLYISDQAEKLRQLAGSDQPSAKIAAIAVMGKSGDLSYVPTLIYALGDSNPDVVIAAD
                     RALRRLTRTFGSGLQPGFSETERQAAIEQWKKWYRQIGPSADWED"
     gene            24838..25692
                     /locus_tag="PLD05_02510"
     CDS             24838..25692
                     /locus_tag="PLD05_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76332.1"
                     /translation="MEVTYRQPWTDSSYGLRVTAFDRAVSMLLALLILLGLCVIGLFI
                     IWVTGRNSSAQTAVPIVLQPIGEGGLPLGSDLDINTENPGQLEDLEEPLLRDALASVA
                     DVVATRLADLDNPVLSDPDSVEAGGTKGELARGSGTGGNGVRRRWEIFFDKGNTLETY
                     ARQLDYFGIELGILLPGNQVQYVSGFSQPRPQVRVGPADAEKRFYLTWQRGELQQADR
                     ELLARAGIDAGNRLILKFLPPEVEARLAALERAYAGGRADQVSSTRFGIRREGNGFAF
                     YVISQQYR"
     gene            25784..26512
                     /locus_tag="PLD05_02515"
     CDS             25784..26512
                     /locus_tag="PLD05_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP76333.1"
                     /translation="MKLDLTAIITYLGTADYIALAVVALWGAFCVVMVLRRIHRVRFA
                     NEQLQEEFLTRVEEGILRNDFESVARFCEEDPRALSQLTRLAIVHRRLGLSRLRTLLA
                     ERFQRDVLSDLEYRLSWIYTVIKSAPMLGLFGTVLGMMGAFAKLSGGEKVDPTKLADD
                     ISLALVTTAIGLAIAIPLVLCTASVSIQIKKLEDYVSSGLNRVLDVLRTAGIKQEITP
                     VPPVGPAVGSGAPRTPAERTFRKE"
     gene            26623..27111
                     /locus_tag="PLD05_02520"
     CDS             26623..27111
                     /locus_tag="PLD05_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP76334.1"
                     /translation="MPKSVWDKIAGEEAEAIVFRRPLQESVDFDITPMIDVTFLLLIF
                     FLVTSAIDVGKAVELPPARFGKGMNPSLALVITVAYQGEATPPAIYLADGKVGSPLSE
                     NAEQQRSDILAAVRESVSAGKPRVLLKAERKVKHKDIARILEIINEVPDVSTYIAVFE
                     RD"
     gene            27259..27783
                     /locus_tag="PLD05_02525"
     CDS             27259..27783
                     /locus_tag="PLD05_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP76335.1"
                     /translation="MGHSQSDDSLIDSSGATEPGETTLSFERVDVPEDAMDMTPMVDV
                     VFQLLIFFMMTAAFSLQKSLELPPSSPQDPTAQSMTLEDLDNESDFVIVRIDHENNIT
                     VDGQDAPSEHELLSKLREAKGGIARTGQAPRTGMLVLADPECRHETVVMVLDAGNAVG
                     MERVRLATVEDTMF"
     gene            28001..31507
                     /locus_tag="PLD05_02530"
     CDS             28001..31507
                     /locus_tag="PLD05_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76336.1"
                     /translation="MTDGRPEVPALAMTNIPAEELIKLLAEKDLVAPEVLEELRNQVA
                     MSRKGPKPIHAAALAKILVDKGYLSRLSAQRLMAKLESDWAQKTHRQKPDPLLLKPKP
                     ATPSPAEGEIRLADEEEPVIDLIALEDDASVPPPVPQKAATAQPPAEAKPQQTEPPVP
                     DEEKSQVDDLLSTLTPATTAPAAAPPVTWERRRKSPWESPLFLLGGGVLLLLVFVGIV
                     LLWSLNRRTGDELLSEADADFHSGSYTQAIYKYGKFLEDFPQHPQAGLARVRSGLARI
                     RQTLESSDPITAFQTVKQIVPEIAALPDFHAEADAEMAAVLPGLAQQIAQQALEKRDP
                     AIVSVGEEALSLCNRYVPKERFPAAKLADIQATLELAKRSISEKDALAATVRQVAECR
                     ASGKLDEAYAIFERFAREYPRSRNDPELQKAMSELAEAEKERVRFEPAQETKSPALPV
                     LPFREFGLYSSWIGGRAPCSESAIAVLGDQECVYAVQMATGQVLWQRFVGGIFAEYPS
                     NTYPRIAVGGQETVAVPDPSSQGVILVDAATGHISRFIRLGEPPASWVLTERSYLLVL
                     GRSGNLLILNPLESQKVGTFRFPQPAAYPPAVDPVTRRAFILGEHSHLFVIRLDSREC
                     EQVVYLGHERGQIVAPPALVSGFLVIPERRGSTEGRLRVVNIQGGATDGLRVVQTIDL
                     PRPVAGPLQVLGTRFVVITSDGQLRVYQLRGGDEKQPFGLLAEGKTPDPTASQKGWAV
                     PRFVLFHKDQVLAADMALTLFELQASAGKLVPRWVACQETLALSSPVVQDSTIFYIYR
                     YPDRPGVFITAVDVESGRVFWESQVADPPLMEAHLTEDGQKAQILGQSGNLYLLEPNA
                     ASSMLAGIRPDRVLRFPGLSEGKAPIGCVLPSKTRVIGILGNRELTVLEPERDRMRVA
                     SLPLPEGLGSAPVPFRNGVLVALEDGTLANLDVTTGRPLGEPYQCEMAAGARVTWSDL
                     VALDETTLLAANSAGWLLKLRWAEKPNGHFQAEHSRLFDAVIASPLAILDDTIIAVDS
                     KMRSLAISLSDLNTQRTQPLDSDLVWGPWSLSTAVCFATADGTLVLSTPQLEFTKVNL
                     EGELPVGKPVVTNNHLVFATRQGSVFCVDFARGTVVNRINLRMTCCTGPTIVGENVLL
                     GSLDGRWIMIPKTDVLHAATKD"
     gene            31488..>32251
                     /locus_tag="PLD05_02535"
     CDS             31488..>32251
                     /locus_tag="PLD05_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76337.1"
                     /translation="MLRPRTKLLLTTFHQLHRKRHAVQAAILAIISFLFCIYLYAEQA
                     KDSSSAGATSPAEQIAEAIKSLIDEKVPLYEQAPFDLIILDAANNHAVLKLAPLALPD
                     RKLPQPLPKTGYLDLELFDEPGERYRLPWRNIVEIRLFERLLIEKATQLIREKRFGEA
                     YDYLDHLSQFYPKAEGLSEVQQLFLYEEAMDALASKTFDRAYANLINLYRLNPQYPAL
                     ADHLSQAAEGLILRYLEIADYASARGVVDSLAQLTP"
     assembly_gap    32252..32351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32352..34081
                     /locus_tag="PLD05_02540"
     CDS             <32352..34081
                     /locus_tag="PLD05_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOP76338.1"
                     /translation="DYASARGVVDSLAQLTPQHPLVQRWRKVWQEQAEDLRTQIEAAL
                     AAGQPRQADRLLRQLRQIWPALTGLPELSNRVQEAYPRLRVAVTLPAISFDPNSLTDW
                     AARRSGRLVYRSLLEYVAPGGEGGVYQSQIIDWKIEELGRRLVFQLRPDRPPFHASGS
                     VYTTYDLAHQLLAMVDPVDQNFTSEWSVLLNRVRMVSPDVVIVELRHAHVRPEGLLRW
                     IPTPGWNLTGTGEALTPENGPFTRSKEESDDKTAVYLRTHSGQQAPGLLEIEEIAFSR
                     GRDAIRALKQGNVDMIDRVNPWELHLLENDASISIASYRVPLVHMLVPNFSRPLPANR
                     DFRRAMLYAIDRPRILKAILNQRNLPGCQVVSGPLPVGRRFDDPLGHGYDTQIAPRPY
                     QPSLALALADLAYRQTVEIAKNRGLPVPARKKLILVHPPHEIAREACSQIKRQWQLIG
                     WETTLVELHPGSPDLPSDFDFLYVEAAVWDPITDLARCFALIRRSQFFPTQVDAALSQ
                     LGQAVDWPDASSRLKTLHRRIYEDTTVLPLWQLTDYYAFRKGIQGIETERATLYQNVE
                     LWTLSDSGR"
     gene            34369..35715
                     /locus_tag="PLD05_02545"
     CDS             34369..35715
                     /locus_tag="PLD05_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76339.1"
                     /translation="MFSPYRVKVFIHFEPGLLSAEDDVHQFHKQVAAVLRPMLGNRWD
                     FEVTGFPTEVISRSLDLRTPPPGDRLPPSWKKITDADKITVVQTIRTGVGWTVQAREW
                     DVRLERWGPLRIVPTSTGSRLCDVVAQGIVSAHVPVGEIQVVDERTVRVAIRGSLLTE
                     ADEERVPSLDACDLFVAVARYEDREGNARAVIPLPWTVLKRSSDVGAEQPTGITCEVV
                     SGIRNALSARRRGRVKLYAQAVQPSGAQPVTLRLQSRDPPRGPLVGYEIYTQPLGEGS
                     PIFVGRTDAAGQAALPAGDASWQLISIKHGRRLLARVPLVPSEGSLAELTLPNDDPRL
                     VAETYLTGLQDELVDLVTLRTILIARMRARMIAEDWEAAQRIRDNLLKLKPREIFSSE
                     LTRQQQRIFCPDPVGQKQIDAMFSEMQRLVNIYLNPQEVESLVKQLASRQKPQPGS"
     gene            complement(35774..36244)
                     /locus_tag="PLD05_02550"
     CDS             complement(35774..36244)
                     /locus_tag="PLD05_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76340.1"
                     /translation="MPDLESVIFSTAVLAIAAAMMWRPAVRFWRRVRFEKARMLFHRE
                     RERLEARLIKQIAVPVVTGEMEWLDCDFEDEVTFLRDRRSGEYVAVVMITLGPETSWA
                     IDSDQESRGRGVAVFRFGKNRWISDPKVYMNLTPEEMVRAFSDRMELVRREMTR"
     gene            36515..>37674
                     /locus_tag="PLD05_02555"
     CDS             36515..>37674
                     /locus_tag="PLD05_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOP76341.1"
                     /translation="MSRRVSLGILLMCLGLIGLCDYASAASPSLWPQWRGPQRDGILS
                     DFTAPEKWPETLKQVWQVTVGSGDSTPALVDGRLYLHTRQGDEEVLLCLDAESGKEIW
                     TNKYPSPPVTGPAARHPGPRSSPAVAQGKVVALGAAGIVSCVDAATGKTLWRNDSFKG
                     VPRFFTAMSPIIVDDVAILHLDGPDTGSVVAFELASGTIRWRWDGDGPSYSSPVLATI
                     EGSRQLVMLTNKNLIGLDVQKGQPLWSVEFAPSGMNYNAATPIVEGSVIYFAGAGRGS
                     RAIRVEKNGSAFSVKEIWNNPELAPQFNSPVLVAGKLFGLSQRGNLYCLDAATGKTLW
                     VDDNRHSGFGAILGSPQALLAMTERNGLIVYAPKAEGFQPLATYQVSAGPVYA"
BASE COUNT         8325 a        10755 c        10096 g         8298 t
ORIGIN      
        1 aacaccacgt caccccagta tccttcgaca ggccaaactg ggtatggcct atacccctca
       61 acgacacttc gttgatccgg gatttgtatg actcattccg aggatatcgc catcgtagca
      121 tctccgcgga agtccaccgg gaacgtgttt ggggtccttg ggaggtagga atcacatgat
      181 gagccaacaa acgccgtctg ccgtgcgcct tcaaaacgcc tttctccgcc tgcaagacaa
      241 gtcacaaggg ctcgttgtta aggagccggc caccactggg ctatcggcca atgctcatga
      301 caccgaggaa acttgtgccg aggcagctac gagctttcgc cccgcattag aggttgacca
      361 attcgctccc cctccgttgg tcagccaact cttggagagc atttcgccca tactggcagg
      421 ttgcctccgt cagatacgcc aaagacttcc taaatccgag cgcgtcgtgg gccttatttc
      481 acctgaaaga ggccatggct gcacaactac agcgatcctg ctcgcgtctc gattatcaag
      541 ggaacgagca actgctctgg tgattgacgc aagcccggac cgggcgctgg ccgactccct
      601 atcgctggag atagagagcg gctgggagca ggccatgctg cgtcggcgcc ccctgagaga
      661 agtgatcatc cgttccaata gtgacggctt tgatattttg ccaggccaac cgggagcgtt
      721 ggcttcaaag gttacgggcg tccaacccac cgattggcca gatctcttaa aggcctacga
      781 ttggatcctc atcgattttg gttcgtcttt tgcccgaacc gcgtattcac ccaccggaac
      841 tgatctgtcg gaggatgatg gcgaagaaag caacgcggtg gtgcgttcgg ggacaggaac
      901 ttcggtacca tttgtctccc tttgctcatc ctttgtcatg gtacaaaggt gcgacagcgg
      961 gaccgcgcgg ttttcgctcc cggccggggc tgccgagatt ctcggtgtga ttgaaacctt
     1021 tgtccagccg acggccgagt ctcaagtctt ggtcaatccc aaagaggccg cgtaaacggg
     1081 tgtcagcgag cagaacagac cgcctcaagg tcgaacgatg tacggaaact actggcattt
     1141 tcgtgaacca ccgtttcgga atcacttccg gcgggccttc ttttttgcaa gcccctccca
     1201 tcaggcctct ctgctgaagc ttcgctatgc cattgagcag cgggaagaag cggccctctt
     1261 ggttggcgaa gctggcaccg gtaagagttt tctcacccac gtgctcaccg aatcgacgaa
     1321 cgaatcggtc gctccggtcg ttcgtacgtg ctgccttttt gatgccccag aagaactgat
     1381 gacacacttg gcggcagagt tcgttggacg gaatttcaac ggctcaggat atgccgctct
     1441 tcaggaactc gaagccttcc tcggacgcaa tcttgaggaa ggccaccatg ccgtgctgat
     1501 cctcgatgac tcgcatctct tgcaaagtat ccgatcgtgg gaagtcctgc gctcgctgat
     1561 gacgctgaag atccagggcg aaacaccctg gaccttgatt gtcctggcac agcccctctt
     1621 cctgccattg ctcgatcgac tgcctcaatt ggaagaattg tttgccttaa aagccttttt
     1681 gcgggcatgg acaccggagg aaacatccca atacatcaag cactgcctga ccacggccgg
     1741 atgcgatgag gatgtctttt ctcccgacgc tgtggaggtc gtagcacggt tttcctccgg
     1801 gataccgcgt cgcatcaatc gcttgtgtga cttgtctctc ctcgtggcgt tcgccgaaaa
     1861 acgctaccaa gtgacggctg cccacgtgga ggccgtccac gaagaactga tgaccgtgcc
     1921 ctgccagtga gagcgatctt caccgccatc agtgcttcag gcccatggta taatcgagag
     1981 catgggcaac gatctgggtt tcatgcgttc gcatctgagt cgcggacttc gtcttcaagt
     2041 tggtcgtcct caaagggcca tttggtgtgg tgtttgtgca gccctcgttg ccgcaggggc
     2101 tctgctcggc acctcgccgt ggacgccgat gacttgggac gaaggtgatg caatccgccg
     2161 cgcggagagc attcgcgatt ggttcagcca tgctttccgc atggaagcca gtggacctcc
     2221 tgccctctcg cgggaaggca ttacgcgcgg ttggccattt accgtccgtc gggaggggca
     2281 tcccgctttg tacggggcaa tcattgccct gggaagaagc ttatcgcccg ggtttctcga
     2341 tcccctcacc aggtatcggt ttggccccat tgtcctattc gccgcggcca tgggggccgt
     2401 aacatatcgc gtcgcccgta tagccggcgt aagccctgct gtggcggcat ttctgtgcat
     2461 ccttcttcaa ccgaggctct ttgctcacgc ccatttcgcc tcgtttgatg gccccctgac
     2521 agcctggtgg atgttggcct gggcgtgctc tcctttcttt tcaaccatgc gaacatcaca
     2581 caaaagaacg tcccgaagcg atctcgcccg catcttcctt tggggcctca tcctcggggc
     2641 aacgatgagt agcaaggcca cagggtggct cgtggttgtg ccctttctct tttgggcgat
     2701 agggcaacgc cagtggagtg tcctttttcg gacgatcgtc ggtatcctta tcggactggg
     2761 aaccttcgtc gtgttgaatc cgccgctctg gtacgatcca atctcgggcg ttttacgctt
     2821 cttccaactt aacctgagtc ggcacgagcg ccccgatctg aatatcacaa cgttcttcct
     2881 gggcgagctt tacaaccttg attatcccct gccatggtac aacacgttgt tctggacagc
     2941 gatcgtggtg cccattccca tcctggcgct cttcatcgtt gggctggtct attgggtccg
     3001 cacatctcgg cgcagtatcc tcacgcttgt tgtcttgcat tggctgacac ttattgtcgt
     3061 ccgcgcgatc cccgggacac ccccgcatga tggcatccgt ttgttcttgc ccgcttttcc
     3121 ttttctgggc atcttggcag cttttgggtt tcgcttcgtc acatccgccc ccatcattct
     3181 tcttcgacga tgggccatgg gatttttgcc actcccacga aggcaaagat caatccgagc
     3241 tgcgcaaccg ttggcggaaa ctagatcctt ctcgtggcgc aggaatttcc tccatacggc
     3301 acactccgtt ttacaaacgg tcatgtggtt gttggttttg acaccgctta ttaacttgat
     3361 ctgttttggg ccccagtggc tttcgtacta caacggcctc attggtgggc tacccggtgc
     3421 cactgccgcc ggtatggaac caacctatta ctgggatggt ctggatcgcg aagtctttct
     3481 gtggcttgaa attcactgct tggagagcga aaaggtctat tattcggcgt gcccgtggac
     3541 gaaccttcgc ttgcttcagg aatggggaat gctcaaacgg gcatttgcta cctcgccggc
     3601 ggatgctcaa tggtacgtcg tgcagaatcg ccccggggca tggttcccgg aagacaagtg
     3661 gctttttact cacggcgaag ccgcatttac caagtaccct ggccgtggtc cgatgtgccc
     3721 ttgggtggga cgggttcccg tcgtcaagat cttccccatt cgagagtatt ttcgcgcttg
     3781 ccaggctgtc cacgcagatc gggcgatgtc tgatcaataa aagctcacac cagaatcgat
     3841 gctcctgcgt cggcatgccc atcttgggga gcgtcatttc ccggcgtatg agcgtctcac
     3901 tgatcgtcga ctcggcacat ccttaacaga tctcactcgg ttgtgcgagg aatttctctt
     3961 ccacgtagcg catgatgagc cgtgcccctt cttggagtgg aaactggcga tcgacgttac
     4021 cctccacaaa ggcaaccttg ttcatcccgt aaacatccga ggtcgcttca tcttggccca
     4081 gcacgtatcc tcctgctgcc cgaatggccc gacatccgtt agctccgtcc cggcccattc
     4141 ccgtcataat gacaccaaga acccgccgcc caaagacttg ggcaacgctc gacatcatga
     4201 catcaacaga gggcttatgg ccgcttacgg gttcgccgtc aaaaatacga atcctagccg
     4261 agccggagcg gccctgcacc gccatatgaa atcccccggg ggcaacgtag gcatggttgg
     4321 gcttgactaa gtctcccgat tcggcctcct tgacagtcaa gcggctgacg gaattgagac
     4381 gccaagcaaa aggttttgtg aactggggcg gcatatgctg aacgacaaga atcgggggta
     4441 atggtggttt aagttcgcga aataggctgg tcaacgcggg ggggccgccc gtcgaaatcc
     4501 cgattgcaat acacgcccgg cttagtgtcg gcggtccttc gagcgactcc gtgggagact
     4561 tgaccacggg ctcggccatg cgcaagcttt gcgcccgttg ttttctgaga cgaacaatcc
     4621 ggttgacatc gctcgttgcg gcaaaacgaa tcttgcggat taactcttgt ccaaagacag
     4681 tcgccgcttc tgacttgtga gctggcttgg tcagataatc aatggcaccg cgctccaggg
     4741 catccaaggt gattgctgcc ccagcctgtg tcaaagagct aaccatgatc acggcaatcg
     4801 gccgtttggc caagagggtg tccaaagtgc ttagcccatc cataccgggc atttcaatat
     4861 ccagggtgat gacgtcgggt gctagttcgt cggccaagcg caatgcctcg cgtccgttgg
     4921 aggccatgcc aacgacctga atccccggtg cggtaaccaa gaaatccttg atcatctgcc
     4981 gcatgacgat cgagtcatcg acgatcagga cacggacggt tttggccatg catgatatcc
     5041 tttcctctct ggcgatttac tctcgggaca ttgatcgagt tgggtcaaac cgtcggccaa
     5101 tccgtctctc acggctaatc gcggaagaaa ccgacgtttc tcgagaaata tggcatattt
     5161 ttcgcagaga aaggtgtcat ctctggattc gcaccgtccg cagttggttt gatcacaaac
     5221 cttattgagg catcaggtga gaaaatcaac aggtttggaa gaatcactag ggatggcaca
     5281 atcgtttcgg ttaactctcc gtgaccttct cacgagtgcc ggaagagctt acgaatggcc
     5341 tcgatcatct cctgatgtgc gccgctttgg gagtactcgc ggagagaaga gagtggcgga
     5401 tgaaggagct tattgatgag acgatcgaat gccaaacgga tttcggcctg aattgcggga
     5461 tccaggtcgg aaagcttgtt aaacagacgc cgcaactctt gctctttgac ggactcccag
     5521 tccttgcgga gctgttgaat caccgggcca accgtccgtt gtcgcaagtt ggctagcacc
     5581 gagccgactt cctgggccac gatctcatcc gcccgaggca attctttatc ccgtgcctcc
     5641 cggttttttt gacacacatc catgaggtcg tccaagcaat aaagatagac gcctggcatg
     5701 gtggcaatcg cgggttcaaa atcccttggc acggccagat caaggacaaa gagcggacgc
     5761 tccaaccgtg cggcgatgac tcggctgagc atttcccgcg tcacgatggg ctgctccgca
     5821 cccgtggtgc tgaccacaac gtccgcctca cggaggacca cagggagctc ctcccagggg
     5881 cgaacttcgc cctggcatcg ttctgctaat tggtgggctc tctccaagtg acgattgatg
     5941 atgacgattc gctgtgcgcc ctcggcacgc aggtagcgaa tcgtttcctc tgccatcttg
     6001 ccggccccga tgaccagcac aagtttgtcc tcaaagcgtt caaaaacggc cctgccgaag
     6061 tcgctgacgg cgatgctggg aatgctgact cgccgttttt ggatttccgt ctccgatgct
     6121 accctccgtg ctgcgcggaa ggccccttgg aatacgatgt gcaaaagagg ccccaccgtc
     6181 cccatttccg tggcccactg gtaagcctgc tttacctgga agagaatctg gggctcaccg
     6241 aggaccatgc tttccagccc cgcggccacg cgaaataggt gttccagcga ctggggagtg
     6301 cagagggcaa aacaaaggtc ggcaaaatct tggcgcgtca gaccgtgaca ttcgccgagg
     6361 aagtcaacga catcggtcgc cggcggaatc gtcgccattt cgctagcaag atagatttcg
     6421 acccgattgc aagtggacag cagaacagct tcaacccagg gaaaccgttt ttcccaaagc
     6481 tgaagcgctg cccgcgtttg ctcgggcgaa aacgccaatt tctcgcgaat ggctaggggt
     6541 gctgtctggt gactgcaccc aacgacacaa atctccatca gtggcctatt ccgtctcaag
     6601 ttcgtgtcac atgttcctcc ggacggttga ggacatcgct atcctcacag caattcttta
     6661 ttcgacgtcc atcccacgaa cataaccctt tcattgcgtg ccagttgctg aatacccctg
     6721 ggtttttttg caatttagga aaccactttt tctcgacatc ccgggctcgg gcattagcga
     6781 aacggcggaa gatttaagac agaaagctct tttctttccg agatacttcc aaggatccat
     6841 cccttgccat tgcgtgcttt taaccggcag gccaccgtcg ggcaattccc gctgttggcc
     6901 atgccgcgta gcgccgaaga gcgtcattcc caataccagg agcatgaaga caaaactcac
     6961 gatggtaaga tagacaactc gtcgcccttc gcggagcggt cgatagacaa agcccaaaag
     7021 cagagatccg cccaaccaga aaaagagaat ccacgtggag agcaccacgg ggtcattcca
     7081 cggcaaatgc ccttgcagcg tgctatattg cacgacgttg agccaaagcc ccgaaaaaat
     7141 accgaccgcc agcatggcca ccgccacaaa aagggcattt ttggtcacat tgtgaagcca
     7201 ctctaagctc ggcagacgaa accgcgaggt cgctatcgcc cgacgcttca aacgatacac
     7261 ctgccctaag tacatcacgc ctgacagcag ggctacgcaa accgccaaga tcgccgtgag
     7321 gatcgagaag ccatggatga tcccccaact tcgtgatgct gccccgcggg caaaaggaac
     7381 cgtatctccc cacacaacac cgagcacgat aagcagcaaa ataaggggca agaaaaacac
     7441 tccaaagggc gtcgctgggc gaatgatcat ccaaaacaaa cagatcaggg ccaatcccca
     7501 ggcaatgccg tagtaccaat cctgccgtat cgagaggacg tgtcctccaa gttccacctg
     7561 ctcttgaaag agggcgattc gatagatgag ccagccggtg tgcactatca ggccgaggac
     7621 caacaatccg ccgatcaagc gcgtggaaac gtgccagcca cgagcccggc ccagttccat
     7681 gagaaacgcc aagccatagg cacacaccat gatggcaatc atttcaccca taacgtacac
     7741 ccaatcactg agacttatca acctccgcaa gacagtctgc cggatgtgtt tgggacctcg
     7801 gtcacatgga gaatatcacg gttccgcgaa ggatcaaaaa gtctcttcgg aagggagagg
     7861 gaggtcgcgg gacgccgacc catcgtcttc cgcttcagtc gcatccaccc aaccccattc
     7921 tttaagcttc ttccgcaagg tgttgcggtt caaccctaat tgagctgccg ccttaatctg
     7981 aacctgatta caccgagcca acacttgatg aatcaactcc ttttcgaccc gtccgacgat
     8041 ctcctggtac aactgtttgt ggtcgggagg aaactgtgcg agagcggcag caacgagatc
     8101 acgcgctagt tcgtccaaat ctttccgacg gtagcccatc cgtggcatac ggcgtcccag
     8161 gacggcgtcc ggcaacaggt cacacgtcac ttcatcgcca ggggccagaa tcacggcacg
     8221 ttccacgtaa ttctgcaact cacggacatt ccccggccaa tcgtagttct tgagggcctc
     8281 catggccttt gggtgcacga aggggacata gcggcggttc ttctcgttgt acagtcgcaa
     8341 gaaatgtccg accagctcgg ggatatcttc ccggcgttca cgcagcggcg gcagatagat
     8401 ggtgaccacg ttgagccggt aatacaaatc ctcgcgaaat cggcctttct cgacctcttc
     8461 cagcaagtcg cggttacttg ccgcgatcac ccgcacatcc acatggatag tccgcgtgtc
     8521 gcccacccgc tcaaactcgt gctgctgaag aacccgcaac agtttgactt gcaacttgaa
     8581 ggttgtggaa tcgatctcgt cgaggaagat cgtccccgta tgcgccgcct cgaaacgccc
     8641 agtccggttt tctatggcgc cggtaaacgc accacgcacg tggccaaaaa gctcactttc
     8701 caaaagcccc tcaggcaggg ccccgcaatt aacgcggaca aacggaccac ttcggcgcgg
     8761 gctgagctgg tggatcgcct tggcgatcaa ctccttgccc gtccctgtct cgccaaggat
     8821 caagaccgag gcatctgagt tggccacgcg acgcgtcaaa cgatagacct cccgcatggc
     8881 cggcgaagag ccaatcaact ccggaatggg aggggaatcg tctggcgtca acacagtggg
     8941 ctccgggttc tatttcgtga ccttgtcgag tcgagcctaa atctacgaat tattttatca
     9001 gccctgccca taagaagagg atgacaggct taacaatctt aggaatcgaa aggggaagcc
     9061 tgtctcacgg tggtaggaaa accatatggc ccgtttggaa gcatccccga aaacgttacc
     9121 acaaaagtca agctctgacc cggggttttt tcattccgtt ttttattcct tagcttgggt
     9181 tcctacagag cgtacctgtg tgggtgcttc gatgcaatcg aggattagtc ccaagacttt
     9241 gcgcacttcc tctggcgact gggcactttg attatatcgc tcgtattgcc ggaggatttg
     9301 ctgggtcgtc agttgaactc cacgtttctc taccgccttt cggaaggctc ccagggcctt
     9361 cacccgttga gcgatcggcc aatcactccg actggcggcc tcgacaagcg ctgactgggc
     9421 cttgggtgtg gccatctggc cgagaatctc taagctaact tcggatagtt gaggaacatt
     9481 gatcgctcgc agggcgacct cctcgatctc catctgcgaa atcccttccg gtgggtgttc
     9541 ggcgagcacg cgcaaccaag ccatggcctt ttgggcaaat tctaaccgct gttgagcgct
     9601 gacacgttga acacccggaa gcatcttagc ccgtgcaatc aaacctgcca aatcttgggg
     9661 atccctcggt gtggcgacac tcagggccac cggaatcgtc cgcaccgtac tatccgccag
     9721 gggtaaccaa tctgtatcgg cataaacgat caccgggacg cgagccaatc gtggctccgc
     9781 ccgcagtcgc tgcaaagtga ggctgagccc aggtcgctgg atcgccgcgg aaacgaggat
     9841 gacttcatag tcagggcttt gccgtgccat ctccaagaga gtacctccgt cggccgcggt
     9901 atccacacgg gcatagccga tcgtcttgag gtaagacgtc atttgggcgg cacgtctcag
     9961 gacaggatca gccacaagaa cgccggctat tccctccgag gaggcaaaat aggcgagttc
    10021 cttggtgacg cggctggccc cccgaaaact tccgcctgct gctaggctca aagctacatc
    10081 tgtcgcaaga aagcggagtt ctggatcagg ggcgtccagg caacgaacca gcgccggaac
    10141 gtctcggccc accaacacgc ccgcgtccgc atactccttc atccagcacg ccacggccca
    10201 tgaagcatgg taccattctc ttgtcagaca ctgattaagc catcggttaa catcctcagc
    10261 cgtcggtttc cctggctcca agatttgctt taccgtatcg gccatcgccc gaatgttggg
    10321 cgccgttgcc gcagacaggt tcgaccgctt tatctcggcc gaatcccatt ctacacgcgc
    10381 gaggtaaccc tctttctcca actggaggca aaccgccaga tcacggagct cgccatcgtc
    10441 cggtgttaat ctcgcagcat gttgggcgag ataggccgct cgctctcgac gccacatctc
    10501 gaacgacacc cgtttcgcaa cgatctgctt tgcggacgca tcccaccgta acagtgtgag
    10561 cacctcatga tccaacggaa ccgactcgat gagtatcgcc tcgacttccg cccgcaaaag
    10621 ccgggcagct tgccgcggtg tgggaagcga ctcacccaac tgctctattg ccctttccgc
    10681 cagttgctgc tctcccctcg ctccctccgg acccatggcc aagcagagga ggaacggggt
    10741 cgcacgggtc acgcccaaag cggcaatgcc cccaatgatc cccgcctgtt tttcctcggg
    10801 caacgtgaaa agtaatgaca aaagggcctc atctgaagcg gggcgaaaat gcagcaaaac
    10861 ataataaact cgaggccaag aggcgcgcca cgctgggtca attaaggcat cacagagcgg
    10921 cttcacggcc gcggactcgg ccttgattaa gtgcttggcc gcccgatacc aacaattgtc
    10981 aggtgcctcg cacagataat tcaccagcat ggccaaccgt tcggcatcgc accgtgtgtt
    11041 acggacggca tcgaacacga gctcggcgag gttcgtgccc tcgggatcca actcaggatg
    11101 gctcgcaaag cggaagaacg tcgcggaacc gaactcccga tcaagcgcca acagcgtggg
    11161 ggggtcaaga gggctttgta aaacgtttgc cagcatctgc ttggccaaat ccgggcggtt
    11221 aagactgagc aacacgtcgg ctgcccgaat tctttcccgc ggtgtcaatg gattggtcgc
    11281 caaaatcgcc gccacagcgg gatcctcttt agccgcgacc tgcatcggat ccgatccgga
    11341 agacgtttgc gccggggcgc catctgcagc cgggccacgc ttggctggtt ccgccggcaa
    11401 aaccttagca gacaagaaaa caaagaccac cgccgcgata accaccttgg tatctcggac
    11461 tgacctcatg acgcggtcct cttggtattg cttcacgtgc gcaaacacat agtcactgag
    11521 cgtggaggcc cccatccacc ggcaaaagcc ttggctttcg aaaacctagc atataggtcc
    11581 aactgatacg gccagaacgg ccccctagta ccccatgata attaacggga caacgattgt
    11641 cctcgaaaat ccagatatgc ctattaaggg tggtgtatta ggcaaccgtg tgggggctac
    11701 gcactaggcc ccaaggtctc gcgccagccg ctcccgccac ctttcaacct gcttgcgaac
    11761 ctcctccgga cacgttgacc cgtaactacg gaatgcttgc acggcgtttt tcacccccaa
    11821 aacggaatag acatttgcat ccagcgaagg atcagccatc cggaacacct cgaggggaag
    11881 gtccgccaaa cggcactggc gatccatggc ctcgcgaacg attcgcccca ccagcgaatg
    11941 cgccgttctc tgcggaatcc cgcgagaaat gaggtactcc atgagcgttg tcgcgtctaa
    12001 gtgtccccgc tccagggact cctcaatgcg ggagcggttc aactcggtca gctccacaag
    12061 gggaatggcc acctccaagc aacccacgac cgtgtcgtag gcgtcaaaga cccccggttt
    12121 atcctcctgt aaatcccgat gatacgccaa aggtagcccc ttcagaagga caaacaactg
    12181 ctgcaagtgg cccaagacac gggctgtttt tccccgaatc agttccaaaa catcgggatt
    12241 gatcttttgc ggcatgatgg aggaacccgt gcaaaatcct tcaggcaatt tcagaaaacc
    12301 gaattcctcc gaggaccaca caatccattc ctcggcccaa tggctgaggt gcactgccag
    12361 caaggccaga tcaaaaacaa attcgacagc aaaatcgcgg tcgctggttg catcgatgct
    12421 gttgggtgac ggctcgtcaa actgcaattc gcgggccgtc atgtgccgat caatgggcaa
    12481 gctagtccct gccaatgcgg cagagccgag cgggcaaatg ttgacgcgct tccggcaatc
    12541 caaaagacgc tgataatccc gctcgaattt ctcgcaataa gccagccaat agtggggtgc
    12601 caggacgggt tgagcccgac gcaggtgggt atatcccggc aagacacagt caaagtcggc
    12661 ttcacagcga ttgacaaacg cggcctggag cttgcgaacg agagggagga tcgtatcgat
    12721 cgcatctcgg acccagagcc ggaaatcggt ggcaacttga tcattacggc ttcgagccgt
    12781 atggagcttc cgccccacat cgcccaatcg ttcgatcaag acccgctcga tgtgcatgtg
    12841 gatgtcttcc caccccacgc ggaactcaaa gtttcccgcg tcgatttcct ctcgaatctc
    12901 ttcaagggcc ttgacgattt gagcggcttc atcccgcgtc agcaatccaa cggcggctaa
    12961 catgcgggcg tgggcgatcg aaccagcaat atcctgtcgg tacagccgac gatcaaagct
    13021 gatgctctcg gtgaacaact ccacacgttg gtcggttgct tcacgaaaaa caccgcccca
    13081 aggtttctct gtcacgggct agctcctcga tattcaaggg acgctggccg aatttggacc
    13141 agcttagtga ctcagacagc caaaagtggg caacaccatg atccaccaag acaccgggaa
    13201 cccaccgccg gctcgcgctc aatgaagatc gtccggtaaa caagccattc ggtggcaaca
    13261 gcggtaaacc ctttttctag ggcgtcgcga agcaatttgt caattgggct aggtgttgcg
    13321 gtgcctttga caggcattct cgggttgtgg caaacgccac caatcgcaga cagggcaacg
    13381 cactaccact caagggtggg ttgcgaatga ccaaaatttc ggcataacat gagaagattt
    13441 agctaatgca ctacgagccg tgcaaagaca cgaatgtcaa cgactttccg caaaaaagtg
    13501 ttgcttaaca aatcgttggt gatcgggggt tcttcgtctc tcctcgccgc ctttggtgag
    13561 aagaggtcca acgattagcc aagagtgctt catcgttgtc ccgttcagag atcgacagtg
    13621 tagggagata gtacggacgt gccacgccgg aacgacattc gcaagatttt gttgatcggt
    13681 tctggaccca ttgtcatcgg tcaggcttgt gaatttgact attcgggttc gcaggcgtgc
    13741 aaagcattgc gtgaggaagg ctacgaggtc gtcctggtca attccaatcc ggcgacgatc
    13801 atgacagacc cggaaatggc cgaccggacc tacatcgaac cgatcacttg ggaattcgtt
    13861 gcgaagatca tcgaaaggga acgccccgac gccctcctcc ccacggtggg aggccagacc
    13921 ggcttgaatg tggccatgga cctttaccgg cacggaatcc tnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn ngtcctgggg tggaaagaga tcgaactgga ggtcatgcgc
    14101 gatgcggacg acaacgtgat cattatctgt tcgatcgaga attttgaccc catgggcatt
    14161 cacacgggcg attcgatcac ggtcgctccc gtccaaacac tgaccgacaa ggagtatcaa
    14221 cgactgcggg acgccgctat tgccgtgatt cgcgaaatcg gcgtcgatac gggtgggtcc
    14281 aacattcagt ttgcgatcca tccgcacaac ggccggatcg ttgtcatcga gatgaatccc
    14341 cgcgtcagcc gctccagtgc tttggcttca aaagccaccg gttttcccat tgccaaaatt
    14401 gccgccaaat tggccgtcgg ctaccgcctc catgaactgg ccaacgacat tacgcgcaag
    14461 accaaggcct gctttgaacc gaccattgac tacgtcgtca cgaaggtccc acgcttcgcg
    14521 tttgagaagt tccccgaagc ggatccaacc ctcaccacgc agatgaagag cgttggggag
    14581 accatggcca ttgggcgaac cttcaaagaa tctttgcaaa aggccttgcg agggttggaa
    14641 gtcggctcat ttggactagg ttgtgacgga aaagacctct ggggcacacc aaagcagccc
    14701 agtattgacg agatccgcta ccaactgtcc caccccaatc cccagcgcat ctggtacatc
    14761 cggtacgcct ttaaggctgg aatgaccgtc gaggaagtca atgagttgac atccatcgat
    14821 ccctggttct tggcaaacat caaggaaatc gttgactttg aaaaccattt gcgaacatat
    14881 cgggacatca gcgaggtcag tgatgagacc ttgagggagg ccaagcggta cggattctcg
    14941 gatcgtcaat tggcccatat ttggtcgcgg gccgagatgg agattcgaga agagagaaaa
    15001 cgccggggga tcgtggccac attcaaggct gtcgatacat gtgcggccga gttcgaggct
    15061 tacacgccct attattattc gacctacgag gatgaagatg agaccccacc caagaagccg
    15121 ggcaaaaagc ggatcatgat tctcgggggt ggtcccaatc gaattggaca aggtattgag
    15181 ttcgactatt gttgttgtca cgccagttac gctctccgtg agttggggat ggaaacgatc
    15241 atggtcaatt ccaacccgga gacggtcagt accgactatg acacgagtga catcctcttt
    15301 tttgaacctt tgacgtgtga ggatatcctc aacatccagg atcgggtcca ggctgatggc
    15361 gtaattgttc agtttggagg tcagacacct cttaatttgg ccagggccct gcaggtggcg
    15421 ggggtcccga tcatcggcac cagtgtggac accattgagg atgccgaaga tcgagaaaaa
    15481 ttcgcagagg tcctgcggcg attgaacttg cgtcaacctc caaatgctac cgcgcggacg
    15541 ttctccgagg cccaattggc cattcaacga attggtttcc cggtcctcgt gcgaccgagt
    15601 tttgtgctcg gtggccgcgc tatggaaatt tgctacgaca tgaaccaatt tgaagaattc
    15661 gtgaaaaagg cctttgatgc cgctgaaggc caacctgtgc tcatcgatcg ctttctggaa
    15721 gatgccattg aggtcgatgt cgatgcgatc gcggatggcg atttggcgat tgtggccggg
    15781 attatggagc acatcgaaga ggcaggcatc cattcaggtg actcggcctg cgctattcca
    15841 ccgtatagct tgccgggacc tgtggtcgaa gagatccgcc aggcaacgca gctcatggcc
    15901 aaggccttga atgtgaaggg gctaatgaac gtgcagtatg ccgtcaaaaa ggaagacggc
    15961 cgaccggtgg tgtatgttct ggaagttaat ccacgggcaa gtcgtaccgt accatttgtg
    16021 tcgaaggcca ttggctggcc tttggcgaag tctgcatcgc tggtgatggt tgggattcca
    16081 ctgcgggaac aaggctggac caaagcccca acgccgacgc gcgtctcggt caaagaggcg
    16141 gtacttcctt tcgtcaagtt ttacggtgtt gatattgtgc tgggaccgga aatgaagtcc
    16201 accggcgagg tgatgggagt cagtcgctac ttctcgattg cctacgccaa gagccaaatc
    16261 gccgccggga cggctttgcc caggtctggt cgaatcttca ttagtgttgc agatcgaaat
    16321 aagcatcaaa ttgtggagct ggctcgccgc ctgcagaact tgggcttcga attactcgcc
    16381 acaaaaggaa ccgccgatcg gttggaacag aacggtatca ctgtgacccg tctcaagaaa
    16441 atcaaagaag gccatcccaa cgtcctcgat tacatcctga atggagatat ccagttgatt
    16501 atcaacaccc ccagcggcaa aggggcgcga acagacgaag gccatattcg ggcgtcagcc
    16561 attgcggcag gtattccttg tatcacgacg atttccgcag ccgaggccgc cgtcaaggct
    16621 atggaggcaa tggcccaaga ccgggacttc gacgttgaag cgatccagga tcgctttccc
    16681 gagtacggcc gaccgcccaa atcgtgagtc ccagtgggca tctccttttt cctatttcgc
    16741 gtatctttgc agaaccaatc gcgccggcag gcaaaaccgc gcggtttctc ctttgagaat
    16801 tggccttgac cctctttcag acctctaggt attctatcct caagaatgaa acttgtgttc
    16861 ggaggccaac gatcatttgc gggtaaaaca acggtttgga gaagactttc gcacacgggc
    16921 cgataacgcc caccgttgtt gggccacgga cggcacgacg caagtgtgac cacagatcgg
    16981 cacgtcagga ggacgactgc ctagtggcga atctggcctc ccatggctcc cggaagagac
    17041 ttctgtcctc ccccctggga cacggccttt ccgggggctt tttttgcgct cacccaggcc
    17101 gcgctacagc ttgacctcct cccacaagtc gctggaggtc tcagcctttg caatgaccgt
    17161 tacgccactt tccgtaacca caaatccccg tgccttatcc ttcgcaggat cgtagccaat
    17221 ttccatccct tccgggatac gaacccgctt atcaatgatg gcgcggcgaa ccctcgcatg
    17281 gcggccaatc tccaccccag tgaacaggat cgagtcctcg acataagcgt agctattgat
    17341 ccgcactcgg tgaccgagga tcgaacgaat aaccgtgccc cctgagacaa tcgtgccgtt
    17401 acatacgata ctatccagtg cctgtcctct gcggtgcgga tcggcaaaga cgaattttgg
    17461 tggcggcagg ttgggctggt aggtgcggat cggccagagt tcgtcgtaaa ggttcagctc
    17521 gggctccacc gagaccaaat ccatattggc ctcgtagtag ctatccaaag tgccaacatc
    17581 ccgccagtat gagtttttct tacggttttc atccatgaag ggataagcga atacccgatg
    17641 cccctcaatc aaggagggaa tgacgtccct gccgaaatcg tgcgtgctcc ctcgccgagt
    17701 ggcgtcctga cagagtatct cgaataaaaa tcgcgacggg aaaacgtaga ttcccatgga
    17761 ggcgtaacag tattcgggct cgccgggaat cggttttgga ttatccggct tttcctgaaa
    17821 accatggacg cgattgttgg tatcgacctc catgacgccg aaacgccgtc gggcggtttc
    17881 gcatcgctct ttcaacacgc caatcgtcac atcggcccca caaccgatgt ggtaatcgac
    17941 gagctttcgg tagtccatct tgtagatgtg gtcccccgct aggacaacca catactcggg
    18001 ctgctccttt tcaatggcat aaatattttg gtaaacagcg tcggccgtcc cttggtacca
    18061 gttctcgtca atccgctgct gaggtggcac aacatcggcg aactcaccca gttcccgaca
    18121 aaaatacttt tgccaaccca tgttgatatg ccggtcgagg ctcatcgcct tgtactgcgt
    18181 taggataaga atctttcgca acccgctgtt aagacaattg gacatcgtga aatcgataat
    18241 ccgataagcg ccgccaaatg gcacggccgg ctttgccctg tcgcgagtca aaggatcaag
    18301 tcgcgtccct tttccccctg ccaaaatgac cgtgacaatc tgttcgttct ttaccgtggc
    18361 catcatctcc ctccttgaca gacgaaccgc taaccagtgc accatcacca ttcaggagac
    18421 gctgctattc gaggattgac cctgttaaga gcctatccca taatctcccc tcgcccgttc
    18481 ggcgggagag gggtcggggg tgagggtgcc tcaaattgct gccgcttttg tcaatcgatt
    18541 ccgatgttcg tttaccctca ccccaaccct ctcccggagg gagagggcga ctttttgaga
    18601 taggctctaa gcaaacttgc tgtcgcggaa ccccctggtg tggttccgag gtttctatgc
    18661 ggcatctttt tcacctgtcc aaattgacag ggcgatgaat cttcaccgcg cagccccttt
    18721 atcccgtcga acgcaacggc acaactctta agccggtctc ttttatgccg ctcccttttt
    18781 cccggctcag tgcttacaaa ctcaccatct accgtctgtc gttcattatt gtatgaaata
    18841 tcaagccaaa gcccaacagt cagacaatta aggcccacct cgttgacgcg gttagctttg
    18901 cgctggataa caaggtgaaa tctcagcgac cgcgtagtca attgatcagc gcagtcctca
    18961 gacgacactt gccaggcagc gtttcagatc ggcaacgtct tccccatccg tccattttcg
    19021 ctgccagagc tcgaaaaaga gaagtgccca aatgcggtgg ctgtggttcc acaagccatt
    19081 gttatgctca ctcaacaatt gttctagtgc ctcacggcga aaataaccgc gagccagcgt
    19141 ccgtgggtcg agcaagatat ggcgagtcaa ctccgacagt gggccgcgga accagtgatc
    19201 cagtggaacg ccaaaaccca tcttgccccg cttttgaatg ggagggggaa tcaaatcacg
    19261 aaatgccgca atcagcacgt gtttcccttt ccgtccccgg agcttcaacc ggccggagag
    19321 tcgcccagcg aactcgacaa attcatgatc taagaacggc gggcgggtct ctaagctatg
    19381 ggccatcgct gcgatatcaa ccttaactag gaggtcgcag ggtaaatagg agaagagatc
    19441 ggccaggcta atggcagaaa caatgtcccg cgaagcgaac cgagagtaga tttccagaaa
    19501 aaaatcctcc ggcacggccg tcaactgctc ttgaaactct ggggtgtaaa gggccaggcg
    19561 gcgggctcgg ttgaagatag caatccagtc aatgtagcgt tgtggtgttg caaggggcag
    19621 agattctgta aagcggtgga ctcgaccaaa gaatgatcgc tgacgcgttc ggggtgccca
    19681 acgccatagt cgctcattaa agatccaacg aatccaacgc ggcaaggacg cgatgccttc
    19741 tgcgaaggcg gttgcccaat accgcgtgta accgcagaac agctcatcgc cgccgtcacc
    19801 cgtcaaggcc actgtcacat gttgtcgggt tttttctgcc acataccacg taggaatggc
    19861 ggaactgtcc gcaaacggct catcgtagtg ccaaacaagg gaaggcaata ctccgatcgc
    19921 atctggattg acgcgatgct ctcgatgttc agtcccaaag gcctcggccg caatccgcgc
    19981 gtaatgggtc tcgtcatatt cggccacagg aaagccaatg gaaaaggtcc ggaccttctc
    20041 ccgggagttc ttttgcatta agcccactac aatcgtcgag tcgatgcccc cggagaggaa
    20101 cgccccgagc ggcacatcgg cctccaaccg catcgtgacg gccttttcca atcgcgagcg
    20161 cagttcttcc ttgatttctt caagcgccat tccgttttcg acgcggctga agtccggatc
    20221 ccaatagcga tggagggtaa gattttttgg ctcgtagatc gccatcgttg cgggaggaat
    20281 cttcttgatt ccccgataga ttgtccacgg atggggcacg tactgataga gaaagtacgc
    20341 atcgacggca aaaggatcaa tttcccgggg aacaccgggt acttccatga gggcctttag
    20401 ttcgctggcg aagagtagcc gttgggaatc cgcataatag acaaggggct tttcccccaa
    20461 gcgatcccgc gccaaaatga gacgccgctt gggctcgtcc cacagggcta atccgaacat
    20521 tccattgacg aattcgagaa aatgaagtcc cttttcctcg taaagatgta gcaggacttc
    20581 cgtatcggaa cggctgcgaa aacgatgacc ggcggcttcc aactcttgcc gtaacgcccg
    20641 gaaattatag atttcaccat taaagacaat ccaaagtgtc tcatcctcgt tggacatcgg
    20701 ctgatgacca ccggccaggt cgatgatgga caatcgtcgg tgccctaggg ccacgccaca
    20761 agccggagca tgaccgtcgg ctgcaatctc tttcaggtaa aagccttgat catctggacc
    20821 gcgatgctgc aacacctcgg tcattcgctg aagcgttgca gcacttatcg cagcggtggg
    20881 ttcataccaa acgccaccgg caattccaca catggagtca cctcgctctt acattcatgc
    20941 gattctgaaa tccctcgccg gagagccggc caacaattcc tgatacacct tttcgtgccg
    21001 gattaccatc tgttccacgg taaattctgt gagcacacgc tgatagcctg cttcacccag
    21061 gcggcgggcc aggtcgggat cttcgaggat ttttttcgta aatcgggcaa ctccggcccg
    21121 aaaatgggga cccaatggca caagaaagcc cgtcacctcg tttaccacca ggtcccgatt
    21181 ccctgggatg tccgatgcca ccacggggac cttcagggtc atggcctcca ggatcgcgtt
    21241 cgattgtccc tcataacggc tcgtattcca tagggcgtca aaatggggca tgattcgcaa
    21301 aacgtcattc cggtgtccca agaagtgcac ccgatcttga atctcaacac tgtttcgata
    21361 ccgaatcaag gcctcacgct ccgggccatc gccaatgata aggagatgga cgtcgctccg
    21421 aatccgtttc aaaaggtccg cagcccaaat tgcgtcgcga acacctttct gcggccaaag
    21481 ccgtccaacc aatccgatca accgggcatc ctgcggcagt cccaattctg cgaggatcga
    21541 ctgccgcgac gtggtatccc gctggggaac cataatcccg ttgggaataa cttcgatctt
    21601 ctctggtgaa atcccacgct gaacataaaa gtcccggacg ccggaactat tcgcaataat
    21661 gcgatcggtg actcgtgcca gtcggcgatc aatccaaaaa tgccatggtt tcttccaaag
    21721 gtccacacac cgttcgctgg caatgatatg cggaacacga gcccgccgtg ctgccaaccg
    21781 accataaacg ttggctgcga aaagccacgt ttgcacgata tcgggtcgga cttctgtgag
    21841 gacccgttgc aaacgcctca aagcgaacaa atcaatcttg cctcgcttgc caattaacgt
    21901 gatgggaatc cccgcccgtt cgaggtcttc tctatacgga ccatcacggg tcaagacgca
    21961 gacgtggaca tccacgcccc gggccttcag ccccgtggct agatagacca tctgcttttc
    22021 ggccccagca cgatccaggg tgggaatgac gtggacagtt ttcatcggct tcaatatcaa
    22081 gcgtattctg caacgctagt agcttgttga tgatcacgcc aatcaggctg ggcgactgac
    22141 atttacaggt tgatcgtttc tggaaatagt caattacatt atccccgcgt acggtagtga
    22201 tgcgtcttgc cgtcttggta gctaagtggt tctcgcctca cccaccgttc tgtgcaagat
    22261 tcttcatttc ctccggagag agcgaggcaa tgtaagggag gttgcggtag tagccccggt
    22321 agtccatacc gtagccgacg acatacacat cgggaatctc aaaccccacc aaagttggtc
    22381 ccaacgggac acgctgccga ttcttctttt tcaataacac ggcagcatga accgaggcga
    22441 ctttgaaatc atcaaactgg ggtatcaact cccagagggt ttctcccgtg tcgtaaatat
    22501 cgtcgataat cagcacgtga cgcccggcaa cttctggggc tagaaggtcg gcctgaatga
    22561 acagcggccc ttggatgttg ccttctgtcg tcacctgtcg cgaggaaatc atttccacgc
    22621 catagggaat cgttagacgc cgcatgagat cggccaaaaa aacgatgctt cctaaaagaa
    22681 caccaaccaa tgtcagcggc tttccccggt aacgtttttc gatctccgcg gccaagcggt
    22741 ccagcccagc ctgaagtgtt gcttcgtcaa tcaggacgtt catagggtac ctctttgcgt
    22801 cctcctccac attcaccaca ttcgtaccgc cataacagaa attaacgttt acccatcgtt
    22861 acgaatgtgt ggaatactac ctgataacga tcatccatgg caagagccga taacgcgggc
    22921 aaccgctccg aacgttgaga cttatggacg tcattgacat tcccacggtt cgggactagg
    22981 atcacatttg ggccggttcg cagctcctgt tgcttgtcat cgagatcact atcgggctgc
    23041 gtttttgcca agggcgaggt cgttaagaaa gaaataggat ggtccgggtg gcggaacagc
    23101 tcctcttcta ttcttgctat tggcggcggc gatttccact gttcgtgtgg gcagcaagcc
    23161 tgatatgggc ttgggcgagt gcattctctc ctatcaaagc ccagctaacg ccagagcacc
    23221 ctgctgttcg gcaggcggtc gaacgtgctc tggcctatct tgccagccga gaagccgagg
    23281 agggctacct ccagattggg gcccgggctt tgagggggtt ggccttcctc aaagccggtc
    23341 atgcccacga tcatcctgtt atcgtcaagt ccgccgaaga gatcatcaaa caaatcccac
    23401 ccagtgggct gcccaaggac tttcgcatga gcaactggga caactacagc accgggttgg
    23461 cgctgatttt cctctgcaat tacgacccac aaaagtttag gcaggagatt cagttaatcc
    23521 ttcaatacag cttcttccgt caaaagaatc acggtgggtg gggctatcct tccaatgaga
    23581 ccggggacac ttcgcagact cagtatcagg ttctcggctt gtgggaagcc atccgcgcgg
    23641 gctttgccgt cccgagtgaa gtcttagaca aggtggccct atggcttgtt tggacacagg
    23701 acccaagcgg aggctacggc taccaaggga acttcccgcc gacacctacc ctgatccccc
    23761 aatctgacgt ccgcattggg atggcggtgg ccggaacggg atccgcatat gtgttggctt
    23821 cactcttacc tggtttccgg ggcacgctgg agcggagcga ccttcctccc gctttggaaa
    23881 aaatcgcaca agcccgtcag gcgggtcgtg ggcgattgac cattgatccc aaactactgc
    23941 gggctgccat acagcgcgga aacgcctata tcgccaagca tttccaagtc gcgcctgacc
    24001 gttaccgcta ctactacatt tacacgctgg agcgatattt cacatttcgc gaagccgtcg
    24061 aaggtccgga gcccggtttg ttcgactggt acaccgcctg cgccgagtat ctcttaaaga
    24121 accagcaaca agatggaagc tggatgcacc agtgcggaaa ggtggtggac acgtctttcg
    24181 ccgtcctatt ccttgtccgc tcgactaaac agagtatgga gggcgtgcgc gactacggtc
    24241 ccggtgtttt ggtcggttct cgaggattac ccaagcagac ggacaacgta attgttaaga
    24301 atggtcaagt ggtggcatta cctctacgaa catctgccga ggatttggca gcggtgctaa
    24361 actctcccga aatgtccgat cttgtttcgg cgatcgaggc tgtcgaggcg atgcctcctg
    24421 aagagatacg cctctatatt tccgatcagg ctgaaaagtt gcggcaatta gcgggcagcg
    24481 atcagccctc tgccaagatc gcagcgatcg cggtcatggg gaaaagtggc gatcttagct
    24541 atgtgcccac gttgatttac gcccttggag actcgaatcc tgacgtggtg atcgctgcgg
    24601 atcgagcctt gcgtcgtctg acgcggacgt tcggttccgg attgcagccg ggtttctctg
    24661 aaacggagcg acaggcagcg attgaacaat ggaagaaatg gtaccgtcag atcggccctt
    24721 cggcagactg ggaggattga gaaggttgga actgttttcc gcgatcggca atgcggcaag
    24781 aaataaagga atctgagcga gagtgtggca cccatttttc tagggtgaat gacccatatg
    24841 gaagttactt accgtcagcc ctggaccgac tcgagttatg gccttcgggt cacggccttc
    24901 gatcgggcgg tcagtatgct tttggcgcta ttgatcctac tcggactttg tgtcattggg
    24961 ttattcatca tctgggtaac ggggcgaaac tcttcggcac aaacggccgt tcctatcgtt
    25021 ctgcagccca tcggcgaggg aggacttcct ttgggctcgg atttggacat caacacggag
    25081 aatcccggac aactggagga cctagaagaa cccctattgc gagacgctct tgcctccgtt
    25141 gccgacgtgg tggccacgcg tctggccgat ctcgacaacc ccgtgctcag tgaccctgat
    25201 tcggtggagg ctgggggcac aaagggagag ttagcccgtg gttcgggcac aggtggaaat
    25261 ggtgttcgcc gtcgctggga aatcttcttc gacaaaggga acaccctcga gacttatgcc
    25321 cgtcaattgg actactttgg cattgaacta ggtatcctct tgccgggcaa ccaagtgcag
    25381 tacgtttcgg gcttcagcca acctcggcct caggtgaggg tgggaccggc cgacgccgaa
    25441 aagcggttct atctgacgtg gcaacgggga gagttacagc aggctgatcg tgaactgctg
    25501 gcccgcgcag ggattgatgc tggcaatcgt ctgatcctta agttcttgcc gcccgaggtc
    25561 gaggcacgtc tcgccgcttt ggagcgggcc tacgccgggg gtagggccga tcaggtgagc
    25621 tccacgcgtt ttggaatccg ccgagagggc aacggtttcg cgttctatgt gatctcgcaa
    25681 caatatcggt aatgcgtctg tccgccttat gccggtgcga tggaacgtta agggtgtcca
    25741 ctcgaacctt tcgaaactat gactagcaga tgacggaaca accatgaaac tggatttgac
    25801 tgccatcatt acctacctgg gaaccgccga ttatatcgcc cttgccgtcg ttgccctgtg
    25861 gggagcattt tgtgttgtca tggtcttgcg aaggattcat cgtgtccggt tcgccaacga
    25921 gcaattgcag gaggaattct tgacgcgcgt ggaagaaggt attctccgca acgactttga
    25981 gtccgtagcg agattctgcg aggaagaccc tcgggcactc tctcagttga cacgtttggc
    26041 gatcgtccat cgccgtctcg gtttatcgcg tttacggaca ttgcttgcgg agcgttttca
    26101 acgcgacgta ctaagcgacc tggagtatcg cctgtcgtgg atctacacgg tcatcaagag
    26161 cgctcccatg ctcggcctct tcgggactgt gttgggaatg atgggggcct tcgccaaact
    26221 ctccggcggc gaaaaggtcg atcccacaaa attggccgat gacatcagcc tggccctggt
    26281 taccacggcc atcggtctgg ccatcgccat tcccctagtc ctttgcacgg cttcggtcag
    26341 tattcaaatc aaaaaactgg aggattatgt cagttctgga cttaatcgcg ttttggatgt
    26401 cttgcgaacc gcgggcatta agcaggaaat cactccggtt ccgccggtcg gtccggcggt
    26461 cggtagcggt gcccctcgca cgcccgccga gaggaccttc cgcaaagagt aaacccagag
    26521 aacggtatct gtgcgaccgc ttagcatgtt tgggaagaaa gtccacacga cgattgctgg
    26581 tcaacagcgt ctatcaaact tggctgtgac gacaggtgtg ccatgcccaa gagtgtttgg
    26641 gacaagatag ccggtgagga ggccgaggcg attgtctttc gccgaccgtt gcaagagtcg
    26701 gtcgatttcg acattacgcc aatgattgac gtgactttct tactgctgat cttttttttg
    26761 gttacgtcag caatcgatgt cggaaaggca gtcgagcttc cacccgcccg atttgggaaa
    26821 gggatgaatc cctcgctggc cctggtgatc acggtagcct atcaaggaga ggcaactccg
    26881 ccggcgattt atcttgccga cggcaaagtg ggtagcccac tctcggagaa tgccgagcaa
    26941 cagcggagcg acattcttgc ggccgttcgg gaaagcgttt cggcgggaaa gccccgcgtc
    27001 ctgctgaaag ccgagagaaa ggtgaaacat aaggatatcg ccagaatcct agagatcatc
    27061 aatgaggtac ccgacgtgag tacctatatt gccgtatttg aacgggattg agcggtgggc
    27121 aaaagaagac aatacgcgtg agcgcggaat ctcatgcatc atgtcgtttg ccaacaatgc
    27181 ggaacaaaaa tagcggtcgc ggacacccta cgcgaccggg agatcatttg cccaaaatgc
    27241 ggcgaaacca ttcgatttat gggccattct cagagtgatg attcattgat cgatagttca
    27301 ggagccactg agccagggga gaccacccta tctttcgaac gagtcgatgt cccggaagat
    27361 gccatggaca tgaccccgat ggtcgatgtg gtgtttcaat tgctcatttt ctttatgatg
    27421 acggcagctt ttagccttca aaagtctttg gaactaccgc catcaagccc acaggacccc
    27481 acggctcaaa gcatgacatt ggaagatctg gacaacgaaa gcgattttgt catcgtgcgg
    27541 attgaccacg aaaacaacat taccgttgac ggccaagatg ccccgagtga acatgaattg
    27601 ctcagcaaac tcagggaagc caagggggga atagcccgca caggacaagc cccccgaacc
    27661 ggcatgctcg tgttggccga tccggaatgc cgtcacgaga cagtcgtgat ggtcctcgat
    27721 gcaggcaacg cggtgggcat ggaacgggtc cgcttggcca cggttgaaga cacaatgttt
    27781 taactctaca agaccgcaag tgacgaggcc gacattaagt cgtttcatca tggcacgacg
    27841 agaatgaggt ggattgtcaa agccgaaaag taactaagag tgttctggaa agccaagagg
    27901 tgctcgacgc gtgaccgttt tcatgatcac tcgagacgct aattaacatc aaccgtggca
    27961 tgtcccggac agtgttttct gtcgaatctg attgagtgta atgaccgatg gtcgtcctga
    28021 agttccggca ctggccatga caaatatccc tgcggaagaa ttgattaaac tgcttgcaga
    28081 aaaagacctc gtggcccctg aggtcttaga ggagttgcgc aaccaagtgg cgatgtcgcg
    28141 gaaggggccc aaaccgatcc atgccgcggc ccttgccaaa atccttgtgg acaaggggta
    28201 cctctcgcga ctatcggcac agcggttgat ggccaaactg gaaagcgatt gggctcagaa
    28261 aacgcatcgc caaaaaccgg atcctttgct cttaaagccc aagccagcga cacccagccc
    28321 ggctgagggt gaaataagac tggctgacga ggaagagcct gttatcgact tgatcgcctt
    28381 ggaggacgac gcctctgtac ccccgcccgt tccgcagaag gccgcaacgg cccagcctcc
    28441 tgccgaggcg aaaccgcaac aaacggaacc gcccgttccc gatgaggaaa agtcgcaagt
    28501 tgatgacttg ctcagcacgt taactccggc gacaacagcc cccgctgcag ccccccctgt
    28561 gacctgggaa cggcgccgta aaagcccctg ggaatcgccg ttgtttcttt tgggaggtgg
    28621 cgttcttctc cttttggtct tcgtcgggat tgtgttactc tggtcactta accgccggac
    28681 gggtgacgag ttgctgtccg aagccgatgc ggattttcat tccggttctt acacccaggc
    28741 catctataag tacggcaaat tcttggagga tttcccccaa catccccaag ccggacttgc
    28801 acgggtccgg agtggcctag cccgaatccg tcaaacactg gagagcagcg acccgatcac
    28861 ggcctttcaa accgtgaagc aaatcgtgcc cgaaatcgcc gcgctgccag acttccacgc
    28921 tgaggccgat gccgaaatgg cggcggttct gcctgggctg gcccaacaaa tcgcccagca
    28981 agccttggaa aagcgggatc cagcgattgt ctcggtgggc gaggaggcat tgagtctttg
    29041 taaccgatat gtccccaaag aacgattccc cgcggcaaag ttggccgaca ttcaagcgac
    29101 gcttgaactg gccaaacgaa gtatttcgga aaaagatgcc ttggcagcca cggtcagaca
    29161 agtcgcagaa tgccgcgcca gtggcaaact cgatgaagcc tatgcgattt ttgaacgctt
    29221 tgctcgtgaa tatccccgct ccaggaatga tcccgaattg cagaaggcaa tgtctgaact
    29281 ggcggaggcc gagaaagagc gggttcgctt tgaacctgcc caggagacaa aatctccggc
    29341 tttgcctgtg ttgccgttcc gggagtttgg tctttattca tcctggatcg gcggtcgggc
    29401 cccctgttca gagagtgcca tcgctgtcct cggtgatcag gaatgcgttt atgccgtgca
    29461 gatggccact ggccaagttc tctggcagcg gtttgtcggt ggtatcttcg cagagtatcc
    29521 ttcgaacacg tatccacgga ttgccgtggg cggccaggaa acggtcgccg tacccgatcc
    29581 atcttcccag ggcgtgatcc tggttgacgc ggcaacgggg cacatcagtc gcttcattcg
    29641 cttgggcgag ccacccgcca gttgggtgct gactgagcga agttacctac tcgtgctcgg
    29701 ccggtcagga aatctcctta tcctcaaccc acttgaaagc caaaaagtgg gaacatttcg
    29761 ctttccacaa ccggcggcct atcctcccgc tgttgatccc gtgacccgcc gggcgtttat
    29821 cctgggcgaa cattcccatc tgttcgtcat tcggcttgat tcgcgagagt gtgagcaagt
    29881 cgtttatctt ggccatgaac gtggacaaat cgtcgcaccg cctgcccttg tttccggttt
    29941 cctcgtgata cccgaacggc gaggttctac cgaaggacga cttcgggtgg ttaatattca
    30001 aggtggtgcg acggacggtc tgcgggtcgt ccaaacgatc gacttgcccc gcccggtggc
    30061 cggtccgctc caggtcctcg gcacgcgatt tgtggtcatt acaagcgatg ggcagttacg
    30121 ggtttatcaa ctccgcggcg gggacgaaaa gcaaccgttc ggtttgcttg cggaaggaaa
    30181 aacgccggat cctacagctt cccagaaggg gtgggcggtc cctcgtttcg tactctttca
    30241 taaggaccag gtgcttgcgg ccgacatggc cttgacgctc ttcgaactac aggcatccgc
    30301 cggaaaactc gttcctcgtt gggtcgcctg tcaggaaacg cttgccctct cgtcccccgt
    30361 tgttcaagat tcaactatct tctacatcta tcgttatccc gatcggccag gggttttcat
    30421 aacagcggtt gacgtggaaa gtggccgtgt tttttgggaa agccaggtgg ccgaccctcc
    30481 gctgatggag gcacacctta ccgaagacgg tcaaaaggcc cagattcttg ggcagagtgg
    30541 aaacctgtat ctccttgagc ctaatgcggc ttcatcgatg ttggccggaa ttcgacctga
    30601 ccgcgttctg cggtttccgg gactttcgga aggcaaggcg cccattggtt gtgtcctgcc
    30661 atcgaaaacg cgggtaatcg gcatcctggg aaatcgagaa cttactgttc ttgaaccaga
    30721 acgcgatcgc atgcgtgttg cgtcacttcc tttgccagaa gggctcggtt ccgctcccgt
    30781 tccgtttcga aatggcgttc tcgtcgcttt ggaggacgga accctggcca acctcgacgt
    30841 gaccaccggg cgcccgctag gtgaaccata ccaatgcgaa atggcagccg gagcaagggt
    30901 cacgtggagt gatcttgttg ccctggacga gacgaccctc ttagcagcca actcggccgg
    30961 gtggctcctc aagctacgtt gggccgagaa acccaacgga catttccagg cagaacacag
    31021 tcgccttttt gatgcggtaa tcgcctcacc attggcgatc ttggatgata ccatcattgc
    31081 cgttgactcg aagatgagga gcttggcgat ctcactgagc gaccttaata cgcagcggac
    31141 acaaccgctg gatagcgacc tcgtgtgggg gccgtggagt ctctccaccg ccgtatgctt
    31201 tgccacggcc gatggcacgc tggtcctttc cacaccccag ctcgaattca ccaaggtcaa
    31261 tttagaaggt gagctccccg tgggtaagcc ggttgtgacc aacaaccatc tcgttttcgc
    31321 cacacgacag ggatctgtct tttgtgttga tttcgctcgc ggcacggtgg tgaaccgaat
    31381 caacctccgt atgacttgct gcactggtcc gactatcgtc ggagaaaatg tcttgttggg
    31441 gagtctggac ggacgctgga tcatgattcc aaaaactgac gtgttacatg ctgcgaccaa
    31501 ggactaaact tcttctgaca actttccacc aactccatcg caaaaggcac gccgtacagg
    31561 cggccattct cgccattatc tcgttccttt tttgcattta tttgtatgct gaacaggcaa
    31621 aagattcgtc ctccgcaggc gcaacatccc cggccgagca aattgccgaa gcgatcaaat
    31681 cgctcatcga cgagaaggtc cccctttacg agcaggcccc cttcgatctg attatcctcg
    31741 atgccgccaa caatcacgcc gtgctcaagc tcgccccact tgctttgcct gaccgcaagc
    31801 tgccccaacc attaccgaag acgggctatc ttgacctgga gcttttcgat gaacccggcg
    31861 aacggtatcg ccttccatgg cggaacatcg ttgaaattcg gctttttgaa cgactgctaa
    31921 tagagaaggc cacgcaactg attcgcgaga aacgcttcgg tgaagcgtat gactatctcg
    31981 accacttatc gcagttttat cccaaggcag agggcttgag tgaagttcag cagctctttc
    32041 tttacgaaga ggccatggat gctttggcat cgaaaacttt tgaccgcgcc tatgccaact
    32101 tgatcaacct ttatcgtctg aatccgcaat atccggctct ggcggatcat ctttcccagg
    32161 ctgccgaagg attgattctc cgttatcttg agatcgccga ttacgcaagt gccaggggag
    32221 tcgtcgattc ccttgcacaa ctgacgcctc annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32341 nnnnnnnnnn nccgattacg caagtgccag gggagtcgtc gattcccttg cacaactgac
    32401 gcctcaacat cccttggtcc agcggtggcg aaaggtttgg caggaacaag cagaggactt
    32461 acgcacccaa atcgaagcgg cattggccgc cggccaacca cgacaggccg atcgtcttct
    32521 ccgtcagctc cgccagattt ggcccgcatt gaccggcttg cccgagctct cgaatcgcgt
    32581 tcaagaagcc tatccgcggc tccgtgtggc cgtaacgttg ccggccatct cattcgaccc
    32641 caattccctc acagactggg cggcgcgacg gtcgggcagg cttgtttatc gttcacttct
    32701 ggaatacgtc gcccccggag gggaaggagg cgtttatcag tcgcagatca tcgattggaa
    32761 gatcgaggaa ttggggcgtc gccttgtttt ccaacttcgc ccggatcgac cgccttttca
    32821 tgcgtccggt tctgtctata ccacatacga cctggcccat cagttgctag ccatggtgga
    32881 tcctgttgat cagaacttca cctccgagtg gtcggtatta cttaatcggg tgcggatggt
    32941 ctctcccgac gtagtcatcg tcgaattgcg acatgcccat gttcgccccg aaggacttct
    33001 ccgctggatt cccactccag gatggaattt gaccggcaca ggtgaggccc tcacgcccga
    33061 aaacggcccg tttacgcgat ccaaagaaga atcagacgat aagacggccg tctatctccg
    33121 cacacattcg ggtcaacagg cccccgggtt actcgaaatt gaggaaatcg ccttttctcg
    33181 aggccgtgat gccatccggg cactgaagca gggtaatgtg gatatgatcg atcgggtaaa
    33241 tccctgggaa ctgcaccttc tcgaaaacga cgcttctatc agcatcgcat cttatcgggt
    33301 tccacttgtg catatgctgg tacccaattt ttcgcggcct ttgcctgcca accgcgactt
    33361 tcgccgtgcg atgctctacg ccatcgatcg cccaaggatt ctcaaggcga ttctcaatca
    33421 acgtaatctg ccaggttgcc aagtcgtcag cggacccctg cctgtgggac gtcgcttcga
    33481 tgatcctctc ggccatggat acgacacgca aattgctcca cgtccctatc agccgtcgct
    33541 ggccctggcc ttagccgatt tggcctatcg acaaacggtg gagattgcga agaatcgcgg
    33601 tctgcccgtg ccagctcgga aaaagctgat cctcgtccac ccaccacacg agattgcccg
    33661 agaggcgtgt tcgcagatta agcgacagtg gcagcttatt ggctgggaaa ccacgttagt
    33721 ggaacttcac ccaggaagtc ccgacctacc atccgatttt gattttcttt acgtggaggc
    33781 cgcggtgtgg gaccccatca cggaccttgc ccgctgtttt gccttgatcc ggcggagcca
    33841 gttctttccc actcaggtag atgcggcact cagtcaactc ggtcaggcgg tggactggcc
    33901 agacgcatca agccggttaa agaccctcca ccgtcgcatt tatgaggaca caactgtgtt
    33961 gccgctctgg caacttacgg attattatgc gtttcgaaaa ggcattcaag gcatcgaaac
    34021 ggaacgtgcg accttgtacc aaaacgtgga attgtggaca ctcagcgaca gcggccgata
    34081 agcgtttcgc caaggaatga caacgaggta ggcggtcagc cccgggcgtt ttgatggatc
    34141 atttggcaaa agcagttgtg ccagtgtgaa acgaaaatga cccttgcatg tccaagaacg
    34201 accatcgcgg ttggtcctca atcgacgaaa gggtttggga gcccttctcc gcggccgggt
    34261 cgcttcattt tttctcatta cgccttgtta cgctttgacc ctcgcctcgt gctttgcggc
    34321 attttcttat ttagcctagc cagctccgct cactcgcagg taacgtggtt gttttcaccg
    34381 tatcgggtca aggtctttat tcacttcgag ccgggtcttt tgtccgccga agacgatgtc
    34441 caccaattcc ataaacaagt ggccgccgtg ttacgtccta tgctcggcaa ccgctgggat
    34501 tttgaagtga ctggttttcc caccgaggtc atctctcgat ctctcgattt acggacaccc
    34561 ccgccgggag atcggttacc accttcgtgg aagaaaatca ctgatgccga caagatcact
    34621 gtggtccaga caatccgaac cggtgtcggt tggaccgttc aggcgcgaga gtgggacgta
    34681 cgcttggaac gatggggacc gcttcgcatc gtgcccactt ccaccgggag ccgtttgtgc
    34741 gacgtggtgg cccagggtat tgtgtctgct catgttcccg ttggagaaat tcaagttgtg
    34801 gacgagcgga ccgtgcgggt cgccatccgt gggagccttt tgaccgaggc agatgaagaa
    34861 cgtgtaccca gcctcgatgc ctgcgaccta ttcgtggccg ttgcccgcta cgaagaccgt
    34921 gaaggcaacg ctcgcgccgt cattccgctc ccttggacgg tcctcaaacg atcgtcagat
    34981 gttggcgctg agcaacctac gggaatcacg tgtgaagttg tttcgggcat tcgcaacgcg
    35041 ttgagtgccc ggcggagagg tcgcgtcaaa ctttatgctc aggccgttca accttcagga
    35101 gcccagcctg tcacattgcg actccagagt cgtgaccctc cacggggacc tttggtaggc
    35161 tatgaaatct acacgcaacc cttgggtgag gggtccccca tattcgtagg acgtacagat
    35221 gccgccggcc aggctgcgct ccccgcaggc gacgcctcat ggcaacttat ttcgatcaag
    35281 catggtcgac gtcttctggc tcgagtgcca ctcgttccta gtgaaggttc ccttgctgag
    35341 ttgacccttc cgaacgatga cccgcgactc gttgccgaaa cctatctcac gggtcttcaa
    35401 gacgaactcg tggatcttgt cactttgcga accatcttaa ttgcgcgaat gcgtgcccga
    35461 atgatcgcgg aagattggga agccgcccaa cgtattcgcg acaatttgct gaagctgaag
    35521 ccgcgggaaa ttttttcgag tgaattgact cgccagcaac agagaatctt ctgtccggac
    35581 ccggttggtc agaagcaaat cgatgcaatg ttctccgaga tgcagcgtct ggtcaacatt
    35641 tacctcaacc cgcaagaagt cgaaagtctt gttaagcaac tggcatcgcg tcaaaagccc
    35701 cagccagggt catagcatca acgaagctgg cctcttggcc gaactacacc ccagggctac
    35761 acgatgcttc gccctatcgc gtcatttcgc ggcggacaag ttccattctg tccgaaaatg
    35821 cccgcaccat ctcctctggc gtgagattca tgtaaacctt gggatcggat atccagcggt
    35881 tcttgccaaa ccgaaacacg gccacgccgc gtccccggga ctcttggtca gaatcgatcg
    35941 cccaggaggt ttcgggccca agtgtgatca tgacaacggc cacgtattct ccgctccgtc
    36001 gatcacggag gaacgtgacc tcgtcctcga agtcacaatc aagccattcc atttcgccgg
    36061 tgacgaccgg aacggcgatc tgcttgatga gacgagcctc caagcgctcg cgttcacgat
    36121 gaaaaagcat ccgcgccttt tcgaatcgga cacgacgcca gaaccgtacc gccggccgcc
    36181 acatcattgc agcggcgatg gccaataccg ccgtcgagaa aatgaccgat tcgaggtccg
    36241 gcatctcaac gcccccaacc ccggaatgag gaccacctcg cctactagca ttctagagag
    36301 ggactccgag gaaatgcaac cagtgggact tccccgttct ttcggagtcc gctcctcacc
    36361 gttgctggat ctgggcgctc tcattgattg acactgtctc gggctataaa ctgatgctca
    36421 aacagaaagc ctttgccaat gaagtaaagt tccaacaagc ggcaaaaggt gggttctgtc
    36481 ttctcgacgg ctcaacttca gaaggaggtc cgccatgtca cgcagagttt ctctcggtat
    36541 tctcctcatg tgcctcggct tgatcgggct ttgtgattat gccagtgcgg catccccgag
    36601 cctgtggccc cagtggcgcg gtccgcaacg ggatggcatt ctctcggact ttacggcacc
    36661 agaaaaatgg ccggaaactc taaagcaggt ctggcaggtc accgtgggtt cgggcgattc
    36721 aacaccagcc ctcgtggatg gacgtcttta cctccacaca cggcaggggg atgaagaggt
    36781 cttactgtgt ttggacgccg aatccgggaa agaaatctgg acaaacaaat accccagccc
    36841 acctgttacg ggaccggcag cccgccaccc tgggcccaga agttctccgg cggtcgccca
    36901 gggaaaagtg gtcgccctag gtgcagcggg gattgtttcc tgcgtggatg ccgctacggg
    36961 taagacgctc tggcgcaacg actcattcaa gggagtgcct cggttcttca cggcgatgtc
    37021 accgatcatt gtggacgacg tggcgattct ccacctggat ggccccgaca ccggatcggt
    37081 cgttgccttc gagttggcta gcggtacgat ccgctggcgc tgggacggtg acggcccctc
    37141 ctattcctcg ccggtgctgg ccactattga aggctcacga cagcttgtga tgcttaccaa
    37201 taagaacctc atcggcttgg atgtccaaaa gggacaacct ctttggagcg tcgaattcgc
    37261 ccccagtggg atgaactaca acgcggcaac cccgattgtc gaaggttctg tgatctactt
    37321 cgcaggggca ggaaggggaa gccgggccat ccgcgtggaa aagaatggtt ctgcgttctc
    37381 cgtgaaagaa atctggaaca atccggagtt ggccccacaa ttcaactcgc cggttttggt
    37441 ggcaggaaaa ctctttgggc tgtcccaacg ggggaatctt tattgtctgg acgccgcaac
    37501 cggcaagacc ctttgggtgg acgacaaccg gcacagtggg ttcggcgcca tcttgggtag
    37561 ccctcaagcg ttgctcgcca tgacagaacg caatggcctg attgtatacg cacctaaggc
    37621 ggagggattc cagccgctgg cgacctacca ggtttctgct gggcccgtct atgc
//
LOCUS       DAOSUT010000016        36946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_784_length_36946_cov_9.647014, whole genome shotgun sequence.
ACCESSION   DAOSUT010000016 DAOSUT010000000
VERSION     DAOSUT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 36946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36946
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_784_length_36946_cov_9.647014"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLD05_02560"
     CDS             complement(<1..522)
                     /locus_tag="PLD05_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP76342.1"
                     /translation="MSTDNTHTMNLGVFLKVFASRAWPDILDAVRAHGLRWIHFTLQA
                     LVGESLPRTINSTTVSEARNDLEKRGLRIASFSGTFNMAHPDADYRQEYLKRFATACQ
                     ACRDLGGSTVALCTGTRDPDDMWRGHPENKSPQAWIIMRDTVAAALEIADRYDLTLAF
                     EPEINNVVDSAEKA"
     gene            730..2430
                     /locus_tag="PLD05_02565"
     CDS             730..2430
                     /locus_tag="PLD05_02565"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOP76343.1"
                     /translation="MPQTGVIHHDAAERLAALANNLYTLPAVALQILELVDRADTDTT
                     VLRQCVEKDPALAAKILRVVNSSLYGPSQPIGDLGQALAFLGVKPLKLLVLGFSLPPR
                     MFQGLEAKVLAYYWRHTLTKAVAARELSEELVRTSSEECFLAGLLQDIGLLVFIQCLG
                     APFQRFVEKVIEKQEDLYAREQEVFGFDHRAISARLLRHWGMPPLIVDIIDPSELPGI
                     SLGASLVQKRKILLAAEFAAKILGDERPEEWPKWENLTKQLRGSCGGPNAEELLKQIY
                     HKVGQLAEVLSLRLPPGQSYEKLLAAAHDQLAKVSEEVAGLMLRREKPQGSSLAESTD
                     HRSGEAEDLHRVLISCLESPSPMLSASLSENPREPIMENHFRLGQSSVVRSNTDRARE
                     GQDAFRTIQAATDDIAKLADPGLLGHLRATVAFCRAKRCGLSLLLADYHNPSDLVMML
                     GEEAFSKLQQFLLTACARLEHPDSLCWPYGDTGCAWILPDCERETAVLYGHQLIRTVG
                     GWRISGRVALSVGLGVGIAFVATPAPNFPAEDLLAAAQRCLFGSLASAGGVVKSIEIY
                     "
     gene            2526..3677
                     /locus_tag="PLD05_02570"
     CDS             2526..3677
                     /locus_tag="PLD05_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOP76344.1"
                     /translation="MRASDRRSPSRKPAAIRVTLTPAPSPAERARGDYGIGSKPILPA
                     GKSPLDYWQRPHEMQKDKRPCWDSDKPIRVAISACLLGQKVRYDGGHKHDPFLTETLG
                     QYVEWIPVCPEVEIGLGIPRPTLRLERRDDQICLVMPKENRDLTHTMQTFARRRVKAL
                     AEADICGYVLKKNSPSCGMERVKVYKPEGDPAGTHVGTFAGVLLEMMPYLPIEEEGRL
                     HDPRLRENWVTRIFAFRRLKNLWSQRWRLNDLVAFHTAHKYLLLAHSPEHYKRLGQLV
                     AHGKSLSREELRTRYEAEFMTGLKRLATPAKNANVLLHMLGFFKKVLPDDVRAEIRGL
                     IDDYRKGSVPLVVPVTLLAHYARRLNITYLLDQVYLYPHPKELALRSHV"
     gene            complement(3856..6513)
                     /locus_tag="PLD05_02575"
     CDS             complement(3856..6513)
                     /locus_tag="PLD05_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76345.1"
                     /translation="MNHDDASTSYQVPPRGLIPIVRFSPVTVRVSVGEATTVQDLVLN
                     RIYEVRVSDNDAGRTITIAPVQTGNVRPGVWLVPNDKKLPTSVTIPVAVFVDEEQPAR
                     QSLWEPRLRAQIAEASRFLEWFCRVRFQVVSAGSWISDNNRTTAEGVLDDFRAKVMSK
                     EARLFIGFSSQITVPADGRFHALPRLLDTHIVLPDVQATFSAQAQLMVLIHALGHFLG
                     AVDTLQEASVMNPDYLIKATEARQPDYFDPLNILIMNIVAEDMMFRGIRQVDQFSTGT
                     KDYLSALYRFIGQRTRRRDVQIFLDALDRPVPARERFLIQWWDGQQTSAPEILQWGSP
                     DAQPEAAGRPLFDEANPVRWILDTSLQDDVTRAEAWLELWAGDRLPGRVEKANPPGVY
                     EKEQLPAHLEVTPYTRVDWPSGPARATIRVLTSQVKRIVWKQVTDELIAGQAFLRDGR
                     SVPYRLVQWNGNEVRLLTSDGIQTIPLSELAELHLPAVDPWEEILHQRASLVPDGKGT
                     IVQLETNDGLIATTSLERFVIRSHGDKGLPENWYHCIQPVWSLDGLWVPHRSVVWRRF
                     FSAHEVPLYVISPASYREMCSLGGRWGFHVNRNLNGTIMASGSLVFGWGFGVPSGSEL
                     VFPLAPLVSGFETKVGLDKLVGDGGCIRAAVAIKDKLEHSIYESPLIVGSARVFQVTS
                     PTTMDNDDQAQLILRIDEAHEDRPPAADPWNVRDVADWGEPILLLDRTALATALRQRG
                     PQFIWAWSGWQPRAEDGPPRIIQDLDDTSPNYPVFRWLHVFDKEYAIQQTLTVPTNGR
                     ILAVFVCRPRNTPEYRAEVFANGELLTSSPVPERSGSNLPPPIEADVSQFAGQKTQLR
                     ILLRSKQQTGEIKAEWHAIELWKDDSSRR"
     gene            complement(6753..8147)
                     /locus_tag="PLD05_02580"
     CDS             complement(6753..8147)
                     /locus_tag="PLD05_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOP76346.1"
                     /translation="MKHVWFVLALLVCLPVSAQDFEVGIGRVNITPDKPIRLSGYAAR
                     NKPSVGVLQEIWAKTLAIRDKEGHRIVIITTDLIGFDHDFSQNLFNEAKKRFGLNREE
                     ILLTCSHTHSGPVVGGNLRTMYKLTEEEAATIDEYAKQLHEKLLTAIDQALQDMQPAV
                     LRVGHGQAGFAINRRELRGDNVVLGVNPTGPMDHDVPVLTVSTPNGKLRAILFGYACH
                     NTTIGGTTGEDFYKIHGDYAGVAQAELEQALPGVTAMFTILCGADQNPNPRGAVDYVV
                     QHGKSLAAAVQQVIQGPMEEVKGPIRAAVAEAELEFAPHTREMFQEELEKALAAKDIY
                     REGRARRMLELYDKGQPMRSLALPLQVVRFDKSLTILAIGGETCVEYALRCKREYPHE
                     NLVVIGYANEVMCYIPSKHVLLGGGYEPVSSMIYYGMPGPFAENVEEVIMASIHKLLE
                     RVGLQPTGTHTAAN"
     gene            complement(8200..9576)
                     /locus_tag="PLD05_02585"
     CDS             complement(8200..9576)
                     /locus_tag="PLD05_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76347.1"
                     /translation="MRRFSLLAMVILGFAISCLCAEEPSHSTSFRIATFRCDVTPPPD
                     GHPLIWLVPVKEVETPLEARGVVLDDGQRRYVVCAMDWCGVCNSTYEMFRSALAEGAG
                     TSPDDVALQTVHQHTAPYTDAGAQPFLDEVKSGIRYVDMDFLGIVATRLKESVKESLN
                     HFQVVTQVGFSASQVDRVASNRRAWTPDGRLVTRYSSCKDPKIRDLPEGLIDPLLRTV
                     TFANADGPIVRLHYYATHPQSFYGDPRASSDVPGFARARLEKEEGVFQIYFTGCAGNV
                     TMGKYNDGSREARDQLTERLYQAMVQAARTTKYQPLRSIGWQSVPFRFVTRSDPGYRE
                     EDLRSRLANPSVDPVQRVRAACGLSFFQRAAKSLSLQALYLNDAVIVHLPGECFVEYQ
                     LYAQSLRPDAFVAVAAYGDLGPGYICIAKAFEEGGYEPTATYLAPENENILKETIATL
                     LKVQRTKP"
     gene            10116..10784
                     /gene="purN"
                     /locus_tag="PLD05_02590"
     CDS             10116..10784
                     /gene="purN"
                     /locus_tag="PLD05_02590"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOP76348.1"
                     /translation="MPNESSVTKGHPFRIAVLISGGGTTLKNLIEKVKSGELPVSIVL
                     VISSNPQAKGLQYAEEEGIPAIVVDWNQYRDSNSFSAVIFKHCQEARANLIVMAGFLK
                     KLVIPNEYENRVVNIHPSLIPAFCGQGFYGYRVHQAVLDYGAKISGCTVHFADNLYDH
                     GPIILQRTVPVLDDDTPETLAKRVAAEEFKAYPEAIRLIAEGRVQVEGRKVRILPPPT
                     ESEL"
     gene            10891..>12425
                     /locus_tag="PLD05_02595"
     CDS             10891..>12425
                     /locus_tag="PLD05_02595"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOP76349.1"
                     /translation="MPLDPTLHPDWEIALEAESRMKTCYQLADELGLTRDELLPYGHY
                     IAKVDYLKVMERLKDGPDGKFIEVTGITPTPLGEGKSTTTIGLLQGIGKRKRRVTACI
                     RQPSGGPTMNIKGSAAGGGLSQVVPLAEFSLGLTGDINAVMNAHNLAMVALTARLQHE
                     RNYDDERLNKIGLRRLNIDPTRVEMGWTIDFCAQALRNIIIGIGGRMDGYMMQSRFQI
                     ASSSEVMAILAVAQSLEDLRKRIGQIIVAYDKKDRPITCEDLEVAGAMTAWLRSAINP
                     NLIQSIEGQPCFVHAGPFANIAIGQSSVIGDQVCLKLADYHITESGFGADIGFEKFWN
                     VKCRYLGKIPNVSVITTTIRGLKHHGVAAGAPPCLPGRPIPKEYFEENLAWLEAGAAN
                     LLHHINTVKKSGVHPVVAINVFPTDTQEEIRLLKRIAEQSGARVAVSNHWRYGGDGAL
                     ELADAVLDACEEPVHFRYLYNMDTPLSERIERIAREVYGADGVEYSREAEAKLKEFES
                     NPK"
     assembly_gap    12426..12525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12633..15290)
                     /locus_tag="PLD05_02600"
                     /pseudo
     CDS             complement(12633..15290)
                     /locus_tag="PLD05_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
     gene            16016..16393
                     /locus_tag="PLD05_02605"
     CDS             16016..16393
                     /locus_tag="PLD05_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76350.1"
                     /translation="MDRYSPVNWVTFMLSLALVAVLANPCQRAFAQTQAKSSSSKSSL
                     HLPHLPNPITALENGAKKVASAVGSLIPGKQQSTSSTRYYPPEGSKKKTEKKGLFSWL
                     APKPEPKKSDSVCDWIGREPLLP"
     gene            17176..18060
                     /locus_tag="PLD05_02610"
     CDS             17176..18060
                     /locus_tag="PLD05_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76351.1"
                     /translation="MVPLLVCIFTGWSGITLAVDVPRQVHPWGRFEPGAWKIMRATTE
                     SFEGGAQSVSITETRATLQEITAAGVTVRVEMLVEVAGKRFRPEPQTVRQGFHGELLT
                     SDSKIKDLGSTEVIVQGRSITCQVLEVETVNAGVRTLSKITYSPTVAPYVIQRDSIRT
                     ELAGNRILESSHMEVTILDVPCRVGLRVLSAAQTRTVQTFPRGSVVTLSLVSGDVPGG
                     VVCQTTKETDDTRKLVRRTTLELVDFGLRPGLRYSGGLRWRLLPREVYKLPQEWIQPG
                     LLPETQGITWPAIQANEG"
     gene            18315..19805
                     /gene="guaB"
                     /locus_tag="PLD05_02615"
     CDS             18315..19805
                     /gene="guaB"
                     /locus_tag="PLD05_02615"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOP76352.1"
                     /translation="MFAHEKIIGHAITFDDVLLVPGYSEVVPAEVDVSTQLTPRIRLR
                     IPILSSPMDTVTESAMAIALAQEGGIGIIHKNMSIARQTAEVEKVKRTANGIIDNPAT
                     LPPTASAAEARELMDQLNVSGIPIVLPDRKLVGIVTRRDLRFLGNLNVPVSEVMTKEN
                     LVTATGTITLSEAEKILMGKKVEKLLLVDEDFRLTGLITIKDIDNQRRFPNACKDEYG
                     RLRVGAAVGVHDYQRAESLIKTGVDVLCVDSAHGHSANVLETVRELKRQFSGHNVDII
                     AGNVATYEGARAVIEAGADAVKVGIGPGSICTTRVVTGVGVPQITAIMAAVEAAKATG
                     TPVIADGGIRYSGDITKAIAAGAHAVMLGGLLAGLDESPGQRILYRGRVYKAYRGMGS
                     LGAMVHGSSERYRQGDAHQPEKLVPEGVEGRVPYRGPLGPFVYQLVGGLKAGMGYCGA
                     RNIEELRTKARFIQVSVAGVRESHPHDITITQEAPNYTVDYAGEEH"
     gene            20014..21021
                     /locus_tag="PLD05_02620"
     CDS             20014..21021
                     /locus_tag="PLD05_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76353.1"
                     /translation="MASTASSDGLQWLPPQPSPVNSVTTPLSPVMQLSPTPQLPAITN
                     STSADSETESLAVALEAPIVDETNPAPVDDPMQDPFGDSTQVKSAPLPQHLAPSSPPQ
                     MLSQGNRPEPLEVLPTPPPLISQAAPTQRGPVTTEVLSQGPGEASKSTETRQTQKTPQ
                     ELLEGIPPAPVDTRPMEEQLAQTPPLKPDECPPFGRMRKISEITNDISAKPGKFPTEC
                     PLQEGEFTPRQWEPIVFTWKASGLCHKPLYFEHAAMERYGHNLGPILQYAEAGTHFFL
                     TIPILPYKMGLNPPDECIYPLGYYRPGSCAPWIIDPFPLSVRAGLAEAGLWTGMVFLI
                     P"
     gene            complement(21732..22769)
                     /gene="tilS"
                     /locus_tag="PLD05_02625"
     CDS             complement(21732..22769)
                     /gene="tilS"
                     /locus_tag="PLD05_02625"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOP76354.1"
                     /translation="MSKNNIISCLEERFLASFPLHGWAGCRTLVAVSGGPDSVALLHL
                     LLRCGGPREQIVVGHVNHRLRGHDSELDAEFVAELCHRLGVKLVMRAVDPPSGGGDGL
                     EEAARRLRYGCLREFAEQEGTRFVLTAHTADDQAETILYRLFRGTGLHGLRGIPRSRP
                     FGPAALVRPLLNFRRRELLDYLHELGETYREDRTNDSLTFARNRVRHLVIPAALQVHP
                     AAIDGVLKLGEVAQETWGFIQRQVELAIDACVKSRAKNLVVIDRGKLARLDPFLIQGL
                     LVELWRSQQWPMRDLSRKHILAMCRFVTSSPSKPGSDTSIHLPGHIECRVDEENVILR
                     LLTSRCDEHQA"
     gene            23664..25199
                     /gene="rny"
                     /locus_tag="PLD05_02630"
     CDS             23664..25199
                     /gene="rny"
                     /locus_tag="PLD05_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOP76355.1"
                     /translation="MAILCVLVAGLTFAVIRLLDYLRRKDAESEARRIIEQAERDAED
                     LKRRVQLELREKDLQERAAREREFQKIREELHQRERALEKREDALEEQASQLRRQEQI
                     VETTQRRLTERLEEVNRRKEELQKLLDLQRQVLHEVSGLSREEATKRLLDLLESQLQQ
                     EVGALVLKYEQKLQETCQQKAREILLTAIQRYAAAHTAETTTSTVDIPNDEMKGRIIG
                     REGRNIRAFEKATGVDVIIDDTPGVVIVSAFDTVRREVARIALNKLIADGRIHPSRIE
                     EIVAETQQELDRFIRQQGEAACQEVDVHGLHERLIYLLGRLHFRTSYSQNVLRHSVEV
                     AFLAGMIADEMGLDGALARRCGLLHDIGKAADHEAEGGHPKIGADLLRRYGEPPEVIH
                     AAAAHHEDMRIENPYTVIVTAADACSASRPGARRETLERYIKRMAELESIALGFPGVE
                     QAYAIQAGRELRVMVNARETTDEIAAKICRDIARAFEERLTYPGEIKVTVLREIRCTE
                     IAR"
     gene            25288..26139
                     /locus_tag="PLD05_02635"
     CDS             25288..26139
                     /locus_tag="PLD05_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOP76356.1"
                     /translation="MRLLLVGDVVGKPGRRIVTRALPLLRRHCRLDFIVVNAENAAGG
                     SGLTPELFYEIVEAGANCITMGDHIYSRRQLEAVLRNDSRIVRPANYPPEAPGREFTI
                     LTAANQEPVAVISLLGRVFMRPVDCYLQAADRVLDKLSSLVKVILIDFHAEATSDKQI
                     LGRYLDGRVSAVLGTHTHVPTADEQILPGGTAFQCDVGMTGPHESIIGRRIDRVLYTT
                     RTFEPTPFEVATEDVRLNGTIVEVDPLTGKSLGIWRLCLRESDLTETILSQVAKSVIL
                     PPARRTE"
     gene            26721..27371
                     /locus_tag="PLD05_02640"
     CDS             26721..27371
                     /locus_tag="PLD05_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76357.1"
                     /translation="MAMIALIRKLLALCLCVLPCVGLVGCSALATAMWITGASDVKPE
                     YDGFKDKKVAVVARPVVALTYRDAHVDKELARRIQLILTNRVRKAQWIDQQKVEKWLD
                     EHEWETFPEVGKGVGADLVIGVEIEDFSLYKGQTVYQGTATLRVAVYDCATNQKVYEK
                     SLSNINYPPNSVVPTSEVPESVFRREFLSVLAEAVARLFHSYDPHEFWALDTRTLR"
     gene            complement(27600..28610)
                     /locus_tag="PLD05_02645"
     CDS             complement(27600..28610)
                     /locus_tag="PLD05_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76358.1"
                     /translation="MSERGDRRDFLRRGLVGAAGVTAFGLEEKILLAALQQGANQQPA
                     TQDDRPMPFGQIGNLKISRMLIGGNLIGGWAHSRDLLYVSRLFKAYNTDEKVFETFAL
                     CEAHGINTVQLDPRCMDVFHRYLTERGGKMQALVCVNPDADTGKLKSQMESLLSQGAT
                     FFYTHGGATDQQMMNGRLDVVAKTVEAIKEMGVPGGVGSHSLSVPKACEENGIGADFY
                     VKTLHSDNYWSATPEELREDFGWFKPRRDEPFSFNDNMWCIDPEETIEFMKSVRKPWF
                     AFKVLAAGAIHPRMAFPYVLRNGADFVIVGMFDFQIAEDVAFFKDAYYKLGERRERPW
                     CA"
     gene            complement(28838..30292)
                     /locus_tag="PLD05_02650"
     CDS             complement(28838..30292)
                     /locus_tag="PLD05_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76359.1"
                     /translation="MNGCWLAKRGILFCLIFLGLVTSRFSKTPILGAESSQKPNIILC
                     MADDQGWGDVGYNGHPVLKTPNLDEMAAQGLRLDYFYAAAPVCSPTRGSVLTGRHPNR
                     YGCFSWGHTIRPQEITIAEALKRAGYTTGHFGKWHVGPVRADSQLCPGASGFDRWLST
                     PNFYDLDPLMSDQGRVVRKQGDSSIIAVEAAIEFIRQATKEGRPFLAVVWFGSPHSPH
                     RALPEDKAFYRDQPERLQDFYGEITALDRAVGNLRKFLRELGIHENTLLWYTSDNGAI
                     EVGSTGGLRGRKGTLYEGGIRVPCVIEWPAKIKTPRRSDFPCGTVDIYPTLLDIVGIT
                     IANQPPLDGISLVPLLEGREPPRRDKPLGFWVFPVGGISTPSEKILAQMLEEQEGRAS
                     ASPPPKDEDKITQQYSEDQLLGHAAWLDWPFKLHRIAGKGGAVKWELYNLATDVTEST
                     DLASQEPERVQTMGAALEAWQKSVLRSLNGEDYR"
     gene            complement(30341..32428)
                     /locus_tag="PLD05_02655"
     CDS             complement(30341..32428)
                     /locus_tag="PLD05_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76360.1"
                     /translation="MKRHRFLLVVTAGLLFCEVFSASSEQSLLAQSSLPVVSGDYPRV
                     FFFRQSEGLARQGRLSFDAWEKAFSRLMGIEGKVLDEEVPGTSANNIEFFVNFKRRYP
                     DQLVLLHFNGNARDPRFETEEYFAGHWIYHVGSKILSDVPAEEGTTEIKVEDPSCYLT
                     GIGRYRNSNDDIGLCELDDSGRPDWNRSEQVQLLAVNRDKGTILVKRGCYGTKPRSFA
                     AGRAYAAAHATEGPWGRNSHLLWYYNYATCCPKDKNGRTCSDILVAELGRRFGPGGEL
                     ALFDGLEFDVLHHSPRAGRRPVDCDADGKADGGVIEGVNVYGIGVIRFLEALRARLGE
                     DKLILADGQLDARTQRGFGLVNGIESEGFPWLRDWEMKQWSTGINRHNFWAQFGRPPV
                     FNYINHKYVRPGTQPGVEATPDVPFSIHRLVFAAAVMTDAAICYSFSPPKDPSSVIGV
                     WDEFWAGQERRLGWLGRPLAPTQTLATQAPDLLAPVLNQLKQKNANFGVLGARTRWEE
                     SGFVVEADEEGKDVVFEIPITISAREAYIECVASAQPRRGYPENMARYATCSLVPTET
                     QDTRTLVGSVGEQGTWVGRKPFSAGFYFPELKPGNYHLRLRVEGAEPVRVERLALYPA
                     ADARYRVFQHGLVLANPSLHEVSYDLRTIAPGRKFRRIKGSPEQDPVTNNGAAVGDMV
                     VLPARDALFLMAE"
     gene            complement(32777..33508)
                     /locus_tag="PLD05_02660"
     CDS             complement(32777..33508)
                     /locus_tag="PLD05_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOP76361.1"
                     /translation="MSECERFSGSDSDQAPTIAGTSGDRVTETLPSHPRVLVAPLHYE
                     PRYSYPLLVWLHGEESEGERQLLEVMPQLDLQYYVAVAPRGVGSNEDFGSNWPQTEEG
                     IVQAASCVFHAMTSAEKRFRIARDKVFIAGHQSGGTMAIRLAWRYPDLFAGAVSLCGP
                     IARTHRPLAQFAKIRRLPVLLTIGRDCDCYGPCQAAEDLKFLHAAGINVMLRQYPGCS
                     ELSGYMLADVNRWMMEIVAGTQIVR"
     gene            complement(33852..>34078)
                     /locus_tag="PLD05_02665"
     CDS             complement(33852..>34078)
                     /locus_tag="PLD05_02665"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661957.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOP76362.1"
                     /translation="EAIIKDKKRLLPCAAYCDREYGVGGYYIGVPVILGKDGVEKIIE
                     LELTPEERAAFEKSVEAVKNLVQTMNRLLR"
     assembly_gap    34079..34178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34318..>35444
                     /locus_tag="PLD05_02670"
     CDS             34318..>35444
                     /locus_tag="PLD05_02670"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOP76363.1"
                     /translation="MDIQQLVSELKEKRGGRIIMMVADGLGGLPLSPGGPTELEAAKT
                     PNLDRLASRGVCGLMTPIKPGITPGSGPGHLGLFGYDPQKYVIGRGVLEATGIGFAVG
                     PKDVAIRCNFCTLDAEGRITDRRAGRIPSEESAAVVSLLKAIRIPGVEVFVEPVKEHR
                     FVVVFRGEGLGANVADTDPQVVGVPPLDPKPLDEESKRTAEIAKQFVTEARKILANQP
                     KANGLTMRGFSKRPDLPTYAEAYGLKAAAIAVYPMYKGLASLVGMDLLGSPTTLDEEI
                     TTLEQAWRETDYDFFFVHFKYTDSRGEDGDFDAKVKMIEQFDAVMPRVEALRPDVLIV
                     TGDHSTPAKLKSHSWHPVPTLLVADTCRPDGAKEFSERACL"
     assembly_gap    35445..35544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(35689..36453)
                     /locus_tag="PLD05_02675"
     CDS             complement(35689..36453)
                     /locus_tag="PLD05_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP76364.1"
                     /translation="MEIHILGSGGYHPSSKRHTLCSVIPQAGVILDAGTAFFRLGRFL
                     DHKPWHVFLTHAHLDHVVGLTYYWGLAQQKEVGPVKIWADESTLKAIKDLLFSQPLFP
                     KIPPYEFIPVSPNESVAIATERAGCESVSVLPVPLSHPGGALGYRFQIGDRSVAYITD
                     TTLEALPVYGQVIQDVDLLIHEAFYPAGYEEMAQRTGHVTVAQAAEVARQVRAKRLVI
                     VHLNPIVDCDGEKEQDEVARIFSNTVIATDNLVVNI"
     gene            36874..36946
                     /locus_tag="PLD05_02680"
     tRNA            36874..36946
                     /locus_tag="PLD05_02680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36907..36909,aa:Ile,seq:cat)
BASE COUNT         8370 a        10428 c         9809 g         8039 t
ORIGIN      
        1 cgccttttct gctgaatcaa cgacattatt gatctccggt tcaaaagcca aagtgaggtc
       61 gtatcgatcg gcaatttcca gggccgcagc cactgtatcg cgcatgataa tccaggcctg
      121 cggcgatttg ttttcagggt ggcccctcca catgtcgtcc ggatctcgcg tccctgtaca
      181 gagggccacg gtcgatcccc caagatcacg acatgcctgg caggctgtgg caaagcgttt
      241 gaggtactct tgtcggtagt ctgcatccgg gtgggccata ttgaatgtgc cggagaacga
      301 cgcaatccgc aatccgcgtt tctccaggtc gttccttgct tcggaaacgg ttgtggagtt
      361 gatagttcgg ggaagcgact cgcccaccag cgcttgaagc gtgaaatgaa tccagcgaag
      421 gccatgggca cgaacggcgt ccagaatatc tggccacgct cgggacgcaa agactttcag
      481 aaaaaccccc aggttcatgg tatgtgtgtt atctgtgctc attttcgctg cttttccaaa
      541 aatcgccatc tttttctttc ggcacgagct agagttgaga tgacagctct catttggatt
      601 acccatgatc caaagcaatt ccctggacga tcgaaaatac cgtttccctt agtaaaacca
      661 atatcaccgt cataatcgat ccggcaaacc aaccgtgtgg aattgagcac atcgtttggc
      721 gtatcaggaa tgccccaaac tggagtaata catcacgatg cggcggagcg attagcagcg
      781 ctggcgaata acctttacac cttgccggcg gtggccttgc aaatccttga gctggttgac
      841 cgcgcggaca cggacaccac cgtccttcgg caatgtgtgg aaaaagaccc ggccttggcc
      901 gctaagattc tgcgtgttgt taatagttcc ctttatggtc cgagtcagcc gatcggcgac
      961 ctaggccaag cactggcgtt cttgggcgtc aagcccctca agctgttggt tttgggcttc
     1021 agcctgcctc cgcggatgtt ccagggcctc gaggccaagg tcctcgccta ctactggcga
     1081 cataccctca caaaagccgt tgcggcccgc gaattgagcg aggagctcgt acggacatct
     1141 agtgaagagt gctttctagc aggtctcctc caagacattg ggctactggt ttttatccaa
     1201 tgtcttgggg ccccttttca aagattcgtg gagaaagtga tcgagaaaca ggaagacttg
     1261 tatgcccgtg agcaagaggt gtttggcttt gaccatcggg cgatctcagc aagacttctg
     1321 cgtcactggg gcatgccacc gctcattgtg gacataatcg acccgtctga actaccaggg
     1381 atctcgcttg gtgctagtct tgtccagaag agaaagatcc tcctggctgc cgaattcgcc
     1441 gccaagattt tgggcgacga acggcctgag gagtggccca agtgggaaaa cctgaccaag
     1501 cagttgcgag ggtcgtgcgg tgggccgaat gctgaggagc tactgaagca gatttatcac
     1561 aaagttggtc aactcgccga ggttctttct ctccgacttc caccgggaca aagctatgaa
     1621 aagcttttgg ccgccgcaca cgatcagttg gccaaggtgt ccgaggaggt ggccggattg
     1681 atgctgaggc gtgaaaaacc tcaaggttcg tccctggcgg aatcgaccga ccaccgttcc
     1741 ggcgaagccg aggacctgca tcgcgtcctg atctcttgtc tggaatcccc ctcgccaatg
     1801 ctttctgctt ctttgtcgga gaacccacgc gaacccatta tggagaacca ttttcggctg
     1861 ggtcagtcgt cggttgtccg aagtaacact gacagagccc gcgaaggcca agacgccttt
     1921 cgtacaatcc aagcagccac agacgatatt gccaaactcg cagatccagg cctattgggt
     1981 cacttgcgtg ccactgtggc cttctgtcgc gcgaaacgtt gtggactcag cctccttctt
     2041 gccgattacc acaacccgtc cgacctagtt atgatgttgg gagaagaggc cttttcgaag
     2101 ttgcagcaat tcttgcttac ggcatgcgcc cgcctggagc atcctgattc tctctgctgg
     2161 ccttacggag atacaggctg cgcgtggatt ctgcctgact gtgagcgcga gacggcagtc
     2221 ctttatggcc atcagttgat ccggacggtc ggcggttggc gaatttcggg ccgggtagcg
     2281 ctttcggttg gtctgggtgt cggcatcgcc tttgttgcca cacctgcacc gaattttccc
     2341 gccgaggacc ttctcgctgc cgctcaacgc tgtctttttg gttcgctggc ctcggctggt
     2401 ggggttgtca agagtatcga aatctactaa aagatcacga tggatttttt gtcgaatcgg
     2461 ctaactgatg ccgcattaag aatctatccc aaaaagtccc cctctccctc cgggagaggg
     2521 ttggggtgag ggcaagcgat cgtcggagtc catcgagaaa accggcagcg attcgagtca
     2581 ccctcacccc cgccccctct cccgccgaac gggcgagggg agattatggg ataggctcta
     2641 agccgattct tcccgcggga aagtctccgc ttgactactg gcaacgacca cacgaaatgc
     2701 aaaaagacaa gcgaccatgc tgggattccg ataaaccgat tcgggtggcc atttctgctt
     2761 gcttattggg gcagaaggtc cgctatgatg gcgggcacaa acacgatccc tttctgaccg
     2821 agacgctggg tcaatacgtg gagtggattc ccgtctgtcc cgaggtggag atcggtctcg
     2881 gtatccctag accgaccctt cgtctggagc gccgtgatga ccagatatgc ctcgtcatgc
     2941 ccaaagaaaa tcgcgacctg acgcacacta tgcagacctt tgcccggcgt cgcgtgaagg
     3001 cgcttgcgga agcggacatt tgcggctacg tcttgaagaa gaattcgccc agttgtggga
     3061 tggagcgtgt caaggtttat aagcccgagg gagacccggc cgggactcat gtcgggacct
     3121 ttgccggtgt gttactagaa atgatgccgt atctccctat tgaggaagaa ggccgcctac
     3181 atgatccccg acttcgcgaa aattgggtta cgagaatttt tgcctttcgt cgcctcaaaa
     3241 acctttggtc gcagcgatgg agattgaatg acctcgtggc ctttcacacc gcccataagt
     3301 atctgctcct ggcacattcc cctgagcatt acaagagatt aggacaattg gtagcccacg
     3361 gaaaatcgct ttcgcgagag gagttacgga cccgctacga agccgagttc atgacaggcc
     3421 tcaagcgctt ggcaaccccg gcgaaaaatg ccaacgtcct ccttcacatg ctggggtttt
     3481 tcaagaaagt cttaccggat gacgttcgtg ccgaaatccg tggactgatc gatgactatc
     3541 gcaagggaag cgttcctctg gtggtccccg ttacccttct tgcccactat gcccggcgac
     3601 taaacatcac gtatcttttg gaccaggttt acctctaccc ccatccaaaa gaactggccc
     3661 taagaagcca cgtgtgaatt ctttctggta ggtactcatt cccgcacgat ggctacaaaa
     3721 cgcagcactc gcgcgggccc gaaagacaag cgtgacggcc gtcgattttc gactacgatg
     3781 tcgttccggg cgaaaactct ggagaatatg cgcctgacaa atttgtagcg gcacatccct
     3841 gtcaccgccg ctgtgttatc gacgagacga atcgtccttc cacagttcga tggcgtgcca
     3901 ctcggccttg atttcgccgg tttgttgttt acttcgcaac aatatcctca attgcgtctt
     3961 ttgaccggca aattgtgaga catcggcctc aattggcggc ggcaaattcg atccactcct
     4021 ttctgggacc ggactgctgg ttagcaactc accattggca aagacctcgg cacgatactc
     4081 cggcgtatta cgcggacggc aaacaaatac cgccagaatt cggccattag tgggaacggt
     4141 tagcgtctgc tgaatggcat actccttgtc gaaaacatga agccaacgga acacgggata
     4201 gtttggcgag gtgtcatcca aatcttgaat tatcctcggc ggcccgtctt cagctcgtgg
     4261 ctgccaaccc gaccaagccc aaataaattg aggccctcgt tggcgcaggg ccgtcgcgag
     4321 ggctgttcga tcaagcaata ggataggttc accccagtct gccacatcac ggacattcca
     4381 tgggtccgcc gcgggaggtc ggtcctcgtg cgcttcatcg atccgtagaa ttagttgggc
     4441 ttggtcgtca ttgtccatag tcgtggggct tgttacttgg aagacgcgag cagaacccac
     4501 aataagaggt gactcgtaga tggagtgctc taatttgtcc ttgatagcaa cagccgcgcg
     4561 gatgcagccg ccgtcgccga ccagtttatc cagtccaacc tttgtctcaa atccgctgac
     4621 cagcggtgcc aaaggaaaaa cgagttcgga accggaaggc acgccaaatc cccagccaaa
     4681 caccaatgac cctgaggcca tgatggtccc gttcagattg cgattgacgt gaaatcccca
     4741 tcgaccaccg agtgagcaca tctcgcggta agaggcagga gatatgacgt acagtggcac
     4801 ttcgtgagcc gaaaaaaacc gccgccacac caccgagcga tggggtaccc aaagcccatc
     4861 gaggctccac accggctgga tgcagtgata ccaattctct ggaaggccct tgtccccatg
     4921 ggaacggatg acgaagcgtt ccaacgatgt ggtagctatc aagccatcgt ttgtctcaag
     4981 ctgaacaatt gtgcctttgc catcgggcac caagctagcc ctttggtgca agatttcttc
     5041 ccacggatca accgcgggaa gatgaagctc cgccaactca gacagtggga tcgtctgaat
     5101 tccatcactc gtgagcagcc gtacttcatt cccgttccat tgcacgaggc ggtatggcac
     5161 gctcctgcca tcacgcaaga acgcctgacc ggcaatcagc tcatccgtaa cctgcttcca
     5221 gacgattcgt ttcacctgag aagtcaacac gcgaatcgtc gcccgagccg gtccggaagg
     5281 ccaatcgaca cgagtataag gcgtgacctc cagatgggcc gggagctgtt ccttttcgta
     5341 gaccccaggc ggattggcct tctcaactct cccgggaagg cgatcgccgg cccaaagttc
     5401 cagccaggct tccgcacggg ttacgtcatc ctgaagcgat gtatcgagaa tccagcgcac
     5461 gggattggct tcgtcgaaca atggccgacc cgcagcttct ggttgcgcat cgggacttcc
     5521 ccattgcaat atctccggag cgctcgtttg ttgtccgtcc caccactgga ttaagaaccg
     5581 ttcccgtgcc ggaaccggtc tatctaaagc gtccaagaag atttggacgt cccgtctccg
     5641 tgttctttgg ccaatgaaac gataaagtgc ggacagataa tctttcgtac ctgttgagaa
     5701 ttggtccacc tgcctgatgc cacggaacat catatcctcg gcaacgatat tcataatcag
     5761 gatattgagc ggatcaaaat agtccggttg gcgggcctcc gtcgccttta ttaggtagtc
     5821 cggattcata accgaggctt cctgaagggt gtccaccgcg ccgagaaagt gtcccaaggc
     5881 atggatcagc accatcaact gagcctgggc ggaaaacgtc gcctggacat cgggcaacac
     5941 aatgtgtgtg tcaagcagcc gaggtaaagc atggaaacga ccatctgcgg gcaccgtaat
     6001 ttggctacta aacccaatga acagcctagc ttcctttgac atgaccttcg cccgaaaatc
     6061 atcaagcacc ccttcagcgg tggtacgatt attgtccgaa atccagctcc ccgcgctcac
     6121 aacctggaac cgaacacgac aaaaccactc caggaagcgc gaagcttccg cgatctgggc
     6181 gcgaagccga ggctcccaca gcgactgccg ggcgggctgc tcttcgtcaa cgaaaaccgc
     6241 gacgggaatc gtcacggatg ttggcagctt cttgtcgttg gggaccaacc aaacgccggg
     6301 acgaacatta cctgtttgca ccggtgcgat cgttatggtc cttcccgcgt cattatccga
     6361 aacgcggact tcataaatcc gattgagcac caggtcttga accgttgtag cttctccaac
     6421 agaaactcga acagtcaccg gagaaaaacg cacgatgggt atcaatccac gagggggaac
     6481 ctgataactg gtcgaggcgt catcgtgatt caataaatcg atccgaatcg cgtggtcgct
     6541 gcgattacta ataacgacga cagccgccag agaagaacgg cccgtttcca agccaagggc
     6601 tagcgcgagc gcaatacaag acaataccgc tcgcggggcg acttggcgcc ttttcccggg
     6661 taactgttgg aaaaacatgc taaatcagta tgaagacaat gattctgtcc gaatgattac
     6721 aacggtggtc agccaatgct cgaccgcagc acctaattgg cggccgtgtg ggtgcccgtt
     6781 ggttgcaggc caacacgctc aagaagcttg tggatggagg ccatgatgac ctcttccaca
     6841 ttctccgcaa aaggccccgg catcccatag taaatcatac tcgaaactgg ctcataccca
     6901 ccaccaagaa ggacatgttt ggatgggatg taacacatca cctcattggc atacccaatg
     6961 acgacaaggt tttcatgagg atattcccgt ttgcaacgga gggcgtactc cacacatgtt
     7021 tccccaccga tcgccaagat ggtgaggctc ttgtcgaacc gcaccacctg caatggcaag
     7081 gctaacgacc gcatcggttg ccccttgtca tacagctcca acatgcggcg ggcacggcct
     7141 tctcggtaga tgtcctttgc agctaaagcc ttttccaatt cttcctggaa catttcgcgt
     7201 gtgtgaggag caaactcaag ctcggcttct gcgacggctg cgcggatcgg gccttttacc
     7261 tcttccatgg gcccttgaat gacctgttgc acggccgcgg ccaatgactt gccatgttgg
     7321 acaacataat caacggcacc tcgcggattt gggttctgat cagcgccgca aaggatcgta
     7381 aacatggccg tcacacccgg cagggcttgc tccaactcgg cctgggcaac gcccgcataa
     7441 tccccgtgaa tcttgtagaa atcctcaccc gtggtcccac caatggtcgt gttatggcat
     7501 gcatagccaa aaaggattgc ccgcagcttc ccgttcggcg tactaacggt caacaccgga
     7561 acatcgtgat ccatcgggcc tgtggggttc acccccagga ccacgttatc tcctcgcaac
     7621 tctcgtcggt taatggcgaa accggcctgg ccatgaccca ccctcaacac ggcgggttgc
     7681 atgtcttgaa gcgcctggtc gattgccgtc agcaactttt cgtgcaattg tttggcatac
     7741 tcatcaatcg tcgctgcctc ttcctcggtc agcttgtaca tggtccgcaa attgccgccg
     7801 accactggac cactgtgggt gtgggagcac gtcaaaagga tttcctcccg gttgagacca
     7861 aatcgcttct tggcctcatt aaacaaattc tgcgagaagt cgtggtcaaa tccgataaga
     7921 tccgtcgtga tgataacaat tcgatgtcct tccttgtccc gaatagccag cgtctttgcc
     7981 caaatctcct gcaaaacacc cacggaaggt ttattccgcg ctgcgtaccc cgacaatcgg
     8041 atcggcttat cgggtgtgat gttgaccctt ccgatcccga cctcgaaatc ctgggcggac
     8101 acaggaagac aaaccaaaag tgccaaaaca aaccatacgt gtttcatagt ttgttctcct
     8161 ctcaacgaac cgttgacgat gtacccccat gcgagttgat tatggttttg tccgctgcac
     8221 tttgagcaac gtggcaatcg tttctttcaa gatgttttca ttctccggag ccaagtaggt
     8281 agccgtcggt tcgtagccgc cctcctcaaa ggccttggca atacagatgt aacccggccc
     8341 cagatcacca tacgcggcca cggccacaaa tgcatctggg cgcaaggact gcgcatataa
     8401 ctggtactcc acaaagcact cgcccggcag atggacaatc acggcatcat tcaagtaaag
     8461 ggcttgtagg gataacgact ttgccgccct ttgaaagaat gaaagcccac acgcggcacg
     8521 aactcgttgc acaggatcga cacttgggtt cgccaaccgg ctacgcaggt cttcttcccg
     8581 atatcctgga tcggaccgag tcacaaagcg aaacggtaca ctttgccagc cgatggaccg
     8641 caagggttgg tactttgtgg ttcgcgcggc ctgcaccatc gcttggtata accgctctgt
     8701 taattgatcg cgggcttcac gactgccatc attgtacttc cccatcgtga cattcccagc
     8761 gcagccggtg aaataaatct gaaacacccc ctcctccttt tcgaggcggg cccgcgcaaa
     8821 acccggaaca tcactactag cccgcggatc tccgtaaaaa ctctggggat gtgtggcgta
     8881 gtaatgaagc cggacaatcg gcccgtcagc gttggcaaag gtcacggttc ttaaaagcgg
     8941 atcaataagc ccttcgggta gatcgcggat tttggggtct ttacatgagc tataccgcgt
     9001 caccaggcgg ccatcgggtg tccaagcccg ccgattcgag gccacgcgat ccacctgtga
     9061 agcactgaat cctacctgag tcacgacctg gaaatggttc agcgactctt tcacagattc
     9121 ctttagccgg gtcgccacaa tccccagaaa atccatgtca acataacgaa taccggattt
     9181 aacctcatcc aaaaatggct gcgcaccagc gtctgtgtaa ggtgccgtgt gttggtggac
     9241 tgtttgtagc gcgacgtcat cgggacttgt gcccgcccct tctgcaaggg cactgcgaaa
     9301 catttcgtaa gttgaattgc atacgccaca ccagtccata gcgcatacga catatcgcct
     9361 ttgaccgtca tccaagacaa cacctctcgc ttcaagggga gtctccacct ctttgacggg
     9421 gaccagccag attaaggggt gtccatcggg cggcggtgtc acatcgcagc gaaacgtggc
     9481 gattcggaaa gatgttgaat gtgacggctc ttcagcacat aggcagctaa tggcaaagcc
     9541 cagaatgacc atggccaaaa gtgagaaacg tcgcatcgtt tttcctccgc tcggctcggc
     9601 atcagacgat aagccaaggc cttcccgtca acccgacatt agtatatcca gaccgttctg
     9661 agtgtccaca ggagcagcgg gagcgaagcc ggtcgtgatt tgacttccac tcatgcgaag
     9721 tgcggccacg acaatcggtg gtcttccatt ggatcgggca gacactaggc ctgcccctcc
     9781 tagcgggtgg aaaacgcacg tccgattaga aaacaccacc atctcaagat gggcgctcac
     9841 cttacagcca cgccatctcg cggtcttcca tcataaggcc tacctacggc atgacgccca
     9901 actttccatt gtggtgtggg cttatcggtc cctcgggcag tatcttaacc acatgcgccg
     9961 tttcattttg gttgtgcgag cgtcttcgga aggcgaggtt aaccgcacgg aggcagttct
    10021 gaaggagcat tgtcagacgt tgacgcggtc attagagtga attacaaatc cttgctaata
    10081 acgctggtgc gagttgacga gataggagat gtgcgatgcc aaatgagtcg tcggtgacaa
    10141 agggccaccc ttttcggatt gcggtcctta tttccggagg cggaacaaca cttaagaacc
    10201 tgattgaaaa ggtgaagtca ggcgaattgc ccgtttcgat tgtcctggtc atatcgagca
    10261 atcctcaggc gaaagggcta caatacgctg aggaagaagg gattccggcg atcgttgtcg
    10321 attggaacca atatcgagat tccaactcgt tcagtgccgt catctttaaa cattgccagg
    10381 aggcgcgagc taaccttatc gtaatggctg gatttttaaa gaagcttgtc atccctaatg
    10441 agtatgaaaa tcgggtcgtg aatattcatc cctcactgat tcccgccttc tgcgggcagg
    10501 gcttttacgg atacagggtt caccaggctg tgttagatta cggagctaaa atcagcggat
    10561 gtaccgtgca ctttgcagat aatctttacg accacggtcc aatcatcctc cagcggaccg
    10621 tgccggtcct ggacgacgac acgccggaga cccttgccaa gcgcgtcgct gcggaggagt
    10681 tcaaggccta cccagaagcc atccgcttga ttgcggaagg tcgtgtgcaa gtggaagggc
    10741 gcaaagtccg catcttgccc ccgcccacgg aatcagaact ttgatgagcg acaccacagg
    10801 cgtgtctgtg cacttgttct ttgccgagtt atattagtcc gccaagtgtc gacaccgatc
    10861 gttaccgttt tagctgctgt gggaaatcgt atgccattgg atccaacgct tcatcccgac
    10921 tgggagattg ccctggaagc ggagtcccga atgaagacgt gttatcagct cgcggacgag
    10981 ctgggattaa cacgtgatga gctattgccg tacgggcact atatcgccaa ggtcgattat
    11041 ttgaaggtca tggaaaggct caaggatggc ccggacggca agttcatcga ggtcacaggt
    11101 attacgccaa cgccgttggg tgaaggcaaa tcgaccacga ccatcgggtt actgcagggg
    11161 attgggaaaa ggaagcgacg ggtgacagcg tgcattcggc aaccgtcggg cggtccaacc
    11221 atgaacatca aaggctcggc tgccgggggc ggactatccc aagtggtacc tcttgccgag
    11281 ttctctctcg gcctaacggg cgacatcaat gccgtgatga atgctcataa tctggccatg
    11341 gtcgccctca ccgctcgatt gcaacacgag cgaaactacg atgacgaacg cctcaacaag
    11401 attggcctca ggcggttaaa catcgatccc acgcgggtgg aaatggggtg gacgatcgac
    11461 ttttgcgccc aggcccttcg caacatcatt atcggcattg gcggtcggat ggacggctac
    11521 atgatgcaaa gccggtttca gatcgccagt tcgtcggagg tgatggccat tttggctgta
    11581 gcccaaagtt tggaggatct gcgcaagcgg attggtcaaa tcattgtggc ctacgataag
    11641 aaggaccggc cgatcacttg cgaagatttg gaggttgccg gcgcaatgac cgcctggcta
    11701 cgaagtgcca tcaatcccaa tctgatccaa tccattgaag gccagccttg ttttgtccat
    11761 gccggtccct tcgccaacat cgccattggc caatcctccg taattggcga ccaagtgtgc
    11821 ctgaaactgg ccgactatca cattacggag tccggctttg gagccgatat cggctttgag
    11881 aagttttgga atgtgaaatg ccgttatcta gggaagatcc ccaacgtctc ggtgatcacg
    11941 acgacaattc gcgggctcaa acaccacggt gttgctgccg gggcaccgcc gtgcttacca
    12001 ggacgtccca ttccgaaaga gtattttgag gaaaacctcg cctggctgga ggccggggcc
    12061 gccaatctgc tgcatcatat caatacggtc aagaaatcgg gtgtccatcc ggttgtcgcc
    12121 atcaacgtat ttcccaccga cacccaagag gagatccgtc tcctcaagcg tattgcagag
    12181 cagagtgggg ctagagtcgc ggtctccaat cactggcgtt acggtgggga tggggccttg
    12241 gagttggccg acgccgtgct cgacgcctgc gaagaacctg tccattttcg ttacctttac
    12301 aacatggata caccactcag tgaacggatc gaacgaattg cccgggaggt ttacggggcg
    12361 gatggcgtgg agtactctcg ggaggcagag gccaagctaa aggaattcga atccaatccg
    12421 aaatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtttg atcggtcgcc
    12541 acttgatgct gtccgttccc ggagatcgac aacgattgag caatcccttt caagctggca
    12601 ggcaacggac ctcccacaac taaccaagga tcagaagcag ccggatttcc ttgcacccct
    12661 ttcccatcct cagacgacac gccctgttcc gttccggaca caggcggctt accggtctgc
    12721 ggctgggtca cctcggagtg agccaccttt tccaattgct tgcgcatgtt ccgctctttt
    12781 tcacgatagc cgggcaagaa catcatcccc agccaccgga agatgtaatc aacgatactt
    12841 gtggccgtgc gaatctcggg atttttggtg aatcccatag gatcaaatcg ggtgtaggaa
    12901 aatttgttca catacacttc cagcggaaca ccatactgaa gactcatgga gactgccgtt
    12961 ccaaagcagt ccatcaagcc cccaatggtg ctgccttctt tcgacatcgt gatgaacaat
    13021 tcaccgggcc ggccatcggg ataaagcccc accgtgatgt aaccctcgtg tccattgatg
    13081 gaaaacttgt gcgtgatgga ccgccgggtg tcggggagtc gttcccgccg tggagccggg
    13141 agtggttcag aggacttttt cgggctggcc tttgacacca atggctggat ttccttggaa
    13201 ccgtcacgat agacagccaa ggccttcaag ccgagacgcc acccctccat gtaggcgttc
    13261 ataatatcgt caaccgtcac atcccgcggc atattgacgg tcttcgaaat agccccggag
    13321 atgaacggct gtgctgccgc catcatgagg atgtgggcct tccagcttat gtagcgcttc
    13381 ccacccggcg gcggtgttgc acagtcgaac accggcaggt gttccggttt gagatggggt
    13441 gccccctcga tgctgtcatg cttctcgatg tattgcacaa tctcgttgat ttggtcctca
    13501 gaatacccca aggtttgtag agccagggag accgtccgat tgacgatctt caaggtcccg
    13561 ccaccggcca attgcttata tttgacaagg gcgaagtccg gctcgatccc tgtcgtatcg
    13621 cagtccatca tgaaactgat tgtccccgtg ggtgcgagaa ccgtcatttg ggcattacgg
    13681 aatccatagc gtcgcccctg ggtcaggacc tcgtcccaaa tctgccgtgc caccttcttg
    13741 aggtattcgg gggcatgctc gagctgctcc accttttccc aatgcatctc catcacacgg
    13801 agcatcggct cgcggttcag ctcgaactcg gcaaaggggc caactgccgc cgccaattcg
    13861 gtacttgtgc ggcaggcagc tccatggagc agggccgtca ccaccccaca cagtcctcgc
    13921 gctgcgtccg aatcgtacgg caagccactg gccataatca ggctgcccag gttggtgtaa
    13981 ccaagcccaa tcggtcggaa gagatggcta ttcttggcga tcttcggtgt cggatagctg
    14041 gcgtgatcga cgaggatttc ttgggcaata aagacaatcc ggcacgccgc gataaatcgc
    14101 tccgcgtcaa acgttccatc ctcccgccgg aatttcatga ggttaacact tgcgaggttg
    14161 cacgcggagt catcgaggaa catatactcc gagcaggggt ttgacgcgtt gatccgaccc
    14221 gatttggggc atgtgtgcca acggttaata gtggtgtcgt actgcaggcc cggatcaccg
    14281 cacatccacg ccgattcggc gatctttcgg agcaagtctc tcgcgcggta tttcggccct
    14341 tccttggtgg ggtctgtcac ccagtgcgtg acccactctc ggtcggcctc cacggcctcc
    14401 atgaattcgt ctgttacccg caccgaaaga ttagcgtttt ggaacagcac ggaactgtag
    14461 gcctcttcgg gcgaatagcc attctggatc aacgttcggg ccttttgttc ctccttggct
    14521 ttacattcga tgaattccaa gatgtcagga tgggtcacct tcaaggattg catcttggca
    14581 gcccggcggg tcttgccccc acttttcacc acagccgcaa tctgatcgta caccctcatg
    14641 aaagacaatg ggcccgaggg ttttccgccc ccagaaagct tttcgcggaa agaccgcaat
    14701 gtggaaaggt ccgtccccgt gcccgacccg aacttgaaaa gcatggcctc gctccgggca
    14761 agctccatga tgtcttccat gttatcctgg acactttgaa taaagcacgc ggacgcttga
    14821 gggtactcgt agggattctc cggctgaacg gccgtctgct tctgcggatc ccaccgccag
    14881 gtgcaactag ctcccttgat accatattgc tgataaagcc ctacgttgaa ccacaccgga
    14941 gagttaaagg ccgcgtactg gtgtaaacag agccaagcca gctctcgata aaatcgctcc
    15001 ccgtcctctt cggtggcaaa atagccgtct tcaatacccc aatcggcaat tgttcggcac
    15061 acccgatgaa tcaattggcg gacactcgat tccctctgtg gggtgcccat ctccccgtag
    15121 aaatatttac tcgccacgat attggtggcc agttggctcc acgattcggg aacttcgcaa
    15181 tccggctgct caaagcacac gccgcctccc tcactcttga tcgtggccgt ccgcttgacc
    15241 catttcacag tgtcaaaagg acttgccaca tcgcttggac aaaaataggc cgcgatgtgc
    15301 aggccctgcc cggggggctg aggtttggca cgattctgct cctgagccgt tactttttca
    15361 gcaagcgtcg aaacctcacg gggatcgtct gtcatgagat ggtctcctta ctcaaaaaag
    15421 ccttccgctt cgcgtgccaa gcctcccaac gaagtcgaag ggaggttgat atacttatgt
    15481 acaccaaact gccgggacaa gcccttagtc ggacattcca ttgccagagt cacttcccga
    15541 agagaaatgc ctccacgtca accagcaaac ctggacctca aagaaggtcc aacacttaga
    15601 aaatcgccgc gtgcctcagt tatccgggtc accccaattc aacccattca tcccaaacga
    15661 cccatgttac ccactatcgg ttgtcacttg gggtagcgat ctccgctttt gggtgggatg
    15721 gcccatccta cttgtgtgtc gcaattatgc gaatctcacc acaaggtatt gtatcatatt
    15781 gcttagccaa acacaagata agccataatc taccacgcca agatcacttg tcaagaggcg
    15841 caggtcaatg attttggttg ctcccgtcta atctgcggaa tccctggcaa atccgccttc
    15901 taccgcccac ggccaatcag cttggcctgg acaaacacac ggcgtttttg gtaactgtta
    15961 caatccgttc cgttgatacg acggtaggac gcaatgtcaa cgtggaggct aggccatgga
    16021 ccggtattcg ccagtgaatt gggtgacatt catgctgtct ctcgcgttgg tggcggtgtt
    16081 ggcaaatccg tgccagcgtg cctttgcaca aacccaagcg aagagttcct catctaagag
    16141 ttccctgcac ctaccgcacc tgcccaaccc cattacagcc ctcgaaaatg gcgcgaaaaa
    16201 agtagcgagc gccgttggct ctctcatccc cggcaaacag caatccactt cctcgacccg
    16261 ttactatccg cctgaaggtt caaagaaaaa aacggagaaa aaaggcctct ttagctggct
    16321 ggccccaaaa cccgaaccca aaaaatctga ttccgtttgc gattggatcg gtagagaacc
    16381 cttgttgccg taatcgctgt ttttgattga gtacctcata gaagagcttt ttatggtatt
    16441 cgcctgagac ccgcaaaacg tggccattgt tttgggatgt tcaatgcagt tcaagcactt
    16501 cccccaactg gttgcttcga ttcttctgct agtcctcgca acggggtgtc ttaccgggtg
    16561 cgccacactc ctatcggcct cagctcaaac cgaccgatcg cttcagcaac gggtggccgg
    16621 tgaccccttt cccacggccc aacaagccgg cattcgctga tcaggttgca acgcaagtga
    16681 aagtggccca cagcacggtg cttagagtct ttcctaaaaa gtccccctct ccctctggga
    16741 gagggttggg gtgagggcaa gccgttgtcg gagtcgatcg agaaaaccga cctcgattcg
    16801 cgcaaccctc acccccaacc cctctcccgc cgagcgggcg aggggagctt gtgggatagg
    16861 ctcttggtaa gcgtgccgcc ggccgcttgc aatgtcactt ttcggggggt ataacaacga
    16921 caaaggcttg gggggtctgg cacggacggg tcgtggaata ccacatcggt ctttctcacc
    16981 agaaaggacg gtcgcttcgt cctcgtgaat cgaataaggt ctcgccacaa agctaacatt
    17041 cccgtcatgg ctctttcatc tgatcagccc gttgccaagc aagtttttat tgcgggtcgt
    17101 ggatcgaatt ccgattacgc tcatttatgt agacgcgatg ggcggtggaa tcgacagcgg
    17161 gccgggtggc cctggttggt cccattactc gtctgcattt tcactggatg gagtggcatt
    17221 acgctggctg tcgatgttcc ccgccaggtg cacccttggg gacgcttcga accgggggct
    17281 tggaagatta tgcgtgccac gacggagtcg tttgagggcg gcgcccagtc tgtaagtatc
    17341 accgagacga gggccaccct gcaagaaatc acggcggcag gggtcaccgt tcgcgtggaa
    17401 atgcttgtgg aggtggcggg gaaaagattt cggccagagc ctcagactgt ccggcagggc
    17461 tttcatgggg aactgctgac ctccgattca aaaatcaagg accttggatc gacggaggtg
    17521 atcgttcaag gtcgatcgat tacatgccaa gtcttggaag tcgaaaccgt caacgcaggc
    17581 gttcggactc tcagcaagat tacgtattca ccgacggttg ctccttacgt aatccaacgg
    17641 gattcgattc gcaccgagtt ggcaggaaat cggatcctcg aatcaagcca tatggaggtg
    17701 acgatcttgg atgttccctg tcgggtcgga ctacgggtcc tttcagcagc tcagactcgg
    17761 acagtccaaa catttccgcg cggctcggtc gtcacgctct ctttagtgtc cggggatgtg
    17821 cccggcggtg tggtatgtca gacgactaag gagacagacg acacaagaaa gctcgtgcgg
    17881 cgcacgacac tggagctggt ggattttggt cttcgcccag ggctcagata cagcggcggc
    17941 ctacgttggc gtttattgcc gcgcgaagtt tacaagcttc cccaggagtg gatccaaccc
    18001 gggttactgc cagaaaccca aggtatcacc tggcctgcca tacaggccaa tgaaggatag
    18061 ccccaccgat tgccaatgac ccgcttcacc gggggccaag caaaccacgg acggtagttt
    18121 gcgccagcgg agggtgtttc ccagcatctc tcctattggc cggagtggag attcacacgc
    18181 cgcttgccta ctacgtgttg tctgtcctac gctctgtttc accccgacgt ccttgtcatc
    18241 tcgatttgct tgttcaatac taaaacattc cccagaaaac acacacgaca cgcacgggtc
    18301 aatggaggcc acccatgttt gcccatgaaa agatcatcgg tcatgcaatc acttttgacg
    18361 atgtcttgct cgtccctggc tacagtgaag tggttccggc agaagttgac gtcagcacgc
    18421 aactgacgcc gaggatccgt cttcgcatcc ccatcttgag ctcgcccatg gacaccgtga
    18481 ctgaaagcgc tatggcgatt gcgcttgccc aggaaggggg gatcggcatc atccacaaga
    18541 acatgtcaat tgcgcgtcaa acggcagagg tggagaaggt caaacgaacg gcgaacggga
    18601 tcattgataa cccggccacg ctcccgccta ccgcctcggc ggcagaggct cgagagttga
    18661 tggaccaact caatgtctcg ggtattccga ttgtgcttcc cgaccggaag cttgtcggga
    18721 ttgtcacccg ccgagacctc aggtttctcg gcaacctcaa tgtgccggtc tcggaagtta
    18781 tgacgaagga gaaccttgta acggccacgg gaactataac gctcagcgaa gctgaaaaaa
    18841 tcctaatggg gaaaaaggtg gagaaacttc tgctggttga cgaagatttt agactgacgg
    18901 gacttataac gatcaaggac atcgacaacc agcggcgttt ccccaacgct tgcaaggatg
    18961 aatatgggcg gctccgcgtg ggggcggccg tgggtgtcca tgactaccag cgagcagaga
    19021 gtttaattaa gacgggagtt gacgtcctat gtgtcgatag tgctcacggt cattcggcca
    19081 acgtgctcga gacagtccgt gagctcaagc gtcaatttag cggccataac gtggatataa
    19141 tcgcgggcaa cgttgccacc tacgaaggcg cccgggcggt gatcgaggct ggggccgatg
    19201 ccgtcaaggt ggggattggt cctggatcaa tttgcaccac acgagtggta acgggcgtgg
    19261 gagtccctca aatcactgcg atcatggcgg ctgttgaggc tgccaaggca acgggaaccc
    19321 cagtgatcgc tgacggagga attcggtatt caggggatat cacgaaagcg attgccgccg
    19381 gggcgcatgc agtgatgctc ggagggctct tggcagggct cgatgaaagt ccaggccaga
    19441 ggatcctata tcgcgggagg gtgtacaagg catatcgggg aatgggttcc ctgggagcca
    19501 tggttcatgg atcaagcgaa cgatatcggc agggagatgc ccaccagcca gagaaattgg
    19561 ttcccgaagg cgtcgaaggc cgggtgcctt atcggggtcc ccttggaccg tttgtttatc
    19621 agttggttgg tggcctgaaa gcagggatgg gttattgcgg tgcccgaaat atcgaggagc
    19681 ttcgcacaaa agcccggttc attcaggtct cggttgctgg ggttagggag agtcatcccc
    19741 atgacatcac gatcacgcag gaagcgccca actacaccgt tgattacgcc ggcgaggaac
    19801 actgattcaa accccttttc gcggaagtca cccttcttcc gcgtagtttc tcggtgtgtt
    19861 ggtttgatca tcgtttttca gtgcttgtgc cccttggtcg agattggggc ctctgaatgg
    19921 acagcccgac ggaagccaac cggagaaaga cgacaagcca tagcaacttt cccatcgtct
    19981 tctaagcaag ttgttcttac ttcgcacgaa gccgtggcat cgactgcaag ttccgacggg
    20041 ttacagtggc ttccaccgca accgtctccg gtcaactccg tgacaacgcc tctttctccc
    20101 gtgatgcagc tttcaccaac accccaacta ccggcgatca caaactccac atccgctgac
    20161 tcggagacag agagtcttgc agtggcatta gaggccccca ttgttgatga aacaaaccca
    20221 gcacccgtcg atgaccccat gcaggacccg tttggggact cgacgcaggt gaagtctgcc
    20281 ccacttcccc agcacctggc gccatcttcg ccaccgcaga tgctaagcca gggcaatcga
    20341 cctgaaccac tggaggtatt gcccaccccg ccgcctttga tatctcaagc ggcgccaacg
    20401 cagcgtggac cagtcacgac ggaggtcctt tcacagggtc cgggcgaggc atcgaaatca
    20461 actgaaaccc ggcagaccca gaaaactccg caagaactct tagaaggtat tccccctgca
    20521 ccggtagaca cccgaccgat ggaggaacaa ctcgctcaaa ccccgcccct gaaacctgac
    20581 gagtgcccac cttttggacg gatgcgaaaa attagcgaga tcacgaacga tatttcggcg
    20641 aaacctggca agttccctac agagtgcccg ctgcaagagg gagaatttac ccctcgtcaa
    20701 tgggaaccca ttgtgtttac ttggaaggcc tccggtcttt gccacaagcc gctatatttc
    20761 gaacacgcgg ccatggagcg gtacggccat aacctggggc ccatcctcca atatgccgag
    20821 gcgggcacac atttcttcct gaccattcct atcctgccct acaagatggg tctgaaccct
    20881 ccggatgagt gtatctatcc gctaggctac tacaggccgg ggagctgcgc tccgtggatt
    20941 atcgatccct tcccactgag cgtccgggca gggctggccg aagctggcct ctggaccggg
    21001 atggtcttcc tcatcccgtg aaaggctgcc attagcctcg cgcagacgcc atttgtcgcg
    21061 gacgttgccc gtaaaatagc gacatcacga agcgagaatc actcgctaaa ctgacactgg
    21121 ccgctacaac agggattagg agttccgcag ggtggcaact gctgtctttc cgcactcttc
    21181 acagagacgg atggcgtact taagagcttg gttaactccg tcgagccaca cttcgggcat
    21241 ttcgccggct cattgccccg gacgagatat tcgaactgat tatgacattt tggacattca
    21301 tattcgtaga ttggcatagc tcgtttcact ccttgtagcg aaccctttgg gccacattca
    21361 tctgttcggg aaatcactcc agcggcgtcc cggtcttcgg gaacatatat gctcggttgg
    21421 cgtcttggca attcgaaagc cctgaccaat aatcgcgcct tgtcgccagc cagcctccgg
    21481 cacacggacc ttcccattac gaaaatgctc gaggccagcc ggaattctgt ggcgccgtgc
    21541 cacggcttgg ccttttttcc cttgccctgt gctgtttctc gaacacatag gtttatagct
    21601 taccgtaccg ttttttgatg cggaaagatc tccgcaagtt acaagggagc acacccgaac
    21661 gaagtctctg cccctatagc cctcgggccg gggcgagcgt gtcttttact gaagccccat
    21721 caccttgctg ttcaagcttg atgctcatca catcgagagg tcaacaatcg caggatgacg
    21781 ttctcttcat cgactcggca ttcaatatgc cctggcaggt gaatggaggt gtcgctcccc
    21841 ggctttgagg gggaggatgt cacgaaacga cacatggcca aaatgtgttt tcgactcagg
    21901 tcccgcattg gccactgctg cgaccgccag agttcgacca aaagcccctg gatgaggaag
    21961 gggtccaagc gggcaagttt cccgcggtcg atgaccacaa gattcttggc tctggattta
    22021 acacacgcgt caatagctaa ctcgacttga cgttggatga aaccccacgt ttcctgagcc
    22081 acctcgccca gttttaggac cccatcaatg gcagccgggt gtacctggag agccgccgga
    22141 atcactaagt ggcgtactcg attgcgggca aaggtgagcg agtcgttcgt tcgatcttcc
    22201 cggtaagtct ctcctagttc gtgaaggtag tccaatagct cccgtcgcct aaagttcaaa
    22261 agaggacgaa ccagggctgc tggaccaaag gggcggctcc gcggaatgcc ccttaacccg
    22321 tgcaaccctg taccacgaaa caagcggtaa agaatcgtct cggcttggtc gtcggcggta
    22381 tgggcggtga gaacaaatct cgtgccttcc tgctctgcaa attcccgtag acagccgtaa
    22441 cgaagccgcc gtgccgcctc ttcaaggcca tccccgcccc cgctgggtgg atccacagct
    22501 cgcatgacga gtttgacgcc caatctgtgg cagagttcgg ccacaaattc ggcatccagt
    22561 tccgaatcgt gtccgcgcaa tctgtggtta acatggccga cgacaatctg ctctcgtggt
    22621 ccgccgcacc gcaggagcag atggagaaga gccacactat ccggcccccc ggagacggca
    22681 accagagtcc ggcaccccgc ccacccatgt agggggaaag aggcgagaaa tcgctcttca
    22741 agacagctaa ttatgttgtt tttcgacacg agaacgaaat tgcttttggc agacaacgac
    22801 aggaaactgg caaatcgaaa gaagtttcgg aaaatgaaga tacggttggt cggaaatctc
    22861 aggttgcggc tatggcatta tgcgtatatc ataatatcgt aagcgtgctt gtttcgaggc
    22921 ataacaaccc ctcgaaccag ccgaatcgtg atttagggac ttgatgtatt ttactacggg
    22981 tagtaaaggc ctgagattcc gaggtatttg ggaactcaga ggaagaagcc gtttgtgtta
    23041 agcgcgatgg cttgacaggc tttcgttggt cgacgctctc gacaagcgaa agtctgttgc
    23101 accgtgagac tggtgtatgg ttgaagctgc cttgttggtc ttcggttttt tgtccctgct
    23161 ttgggtggta cgcatcccca gggctggtgg ccaagcggtt cacctagagc cggcttggta
    23221 cttctgggat gcatattcac tcgtcgggat atcgagtgcc attcgatcgc ggaaccgcag
    23281 catggtcttc tggccgttgg tctcgaagag acgtggggag gtacctctcc ttcgaaggct
    23341 cttggggcca attgaggaaa atgtcaggcc ggaagagacg gaggtcttgc catgcccggc
    23401 cacgggttcg gcacatccct ccgccagtgg ggcgaagccg ttggctttgg catcccgcgg
    23461 tggggacggc ttctcactcg gtttgtgctc acatgggtta gccactagcg tgcggctagg
    23521 acgctcatgg ctgatgaggt ttttcaactt ccggccgagc gttccccggt gagcgtctct
    23581 tcccaatcct ttcggaatcc gtgccctcgc ccggcaagat atgagagggc gcaactgtgt
    23641 ttggagaaaa tcagattctt ttcatggcaa tcctgtgcgt cttagtcgca ggattgacgt
    23701 tcgcggttat acggctatta gactacttgc ggcgtaaaga cgccgaatca gaagcccgcc
    23761 gcattattga gcaggcagag cgggatgccg aggatctgaa acggcgagtc cagttggagc
    23821 tgcgagagaa agacctccaa gagcgcgctg ctcgggaaag agagtttcag aaaattcggg
    23881 aggaacttca ccagcgcgag cgggcacttg aaaagcgtga agacgccttg gaagaacagg
    23941 ccagccaatt gcgacgccaa gagcaaatcg tcgagacgac acaacgtcgc ttgacagagc
    24001 gattggagga ggtcaatcgt cgcaaggaag agttgcaaaa actgttggac ctccagcggc
    24061 aagtcctcca tgaggtcagc ggactctcgc gggaagaggc caccaaacgg ctgttggacc
    24121 ttctcgagtc tcaactgcaa caggaggtcg gtgcactggt cctaaagtac gaacagaagc
    24181 tccaggaaac ttgtcagcaa aaagctcgtg agatcctgct taccgccatt caacggtatg
    24241 ctgccgccca cacagcggag acaaccacca gcaccgttga cattcccaac gatgaaatga
    24301 aaggccggat catcggacgc gaaggccgta acattcgggc attcgaaaag gccaccgggg
    24361 tcgatgtcat cattgacgat acaccagggg tggtaattgt cagcgctttt gacacggtcc
    24421 gtcgagaagt cgcccgaatc gctttgaaca aactcattgc cgatggacgg attcacccgt
    24481 cgcgaatcga agagatcgtt gccgaaacgc agcaggaatt ggaccggttt atccgacaac
    24541 aaggagaggc cgcctgtcag gaagttgatg tccatggact acacgaacgg ctcatttatc
    24601 ttttgggaag gctgcatttc cggaccagct acagccaaaa cgtgttgcgg cattccgtgg
    24661 aggtagcgtt cctcgcaggg atgatcgcgg acgagatggg cctggacggt gccttggccc
    24721 ggcgatgcgg cctccttcac gatatcggta aggcggccga tcacgaggcc gagggaggac
    24781 atccgaagat cggggccgac cttttgcgac gttacggcga accgcccgag gtgattcacg
    24841 cggctgccgc ccatcacgag gacatgcgta tcgagaaccc ctacacggtc attgttacgg
    24901 cagcggacgc ttgcagtgcc tcacgccccg gagctcgccg cgagactttg gagcgctaca
    24961 tcaaacgtat ggcggaactc gaaagtatcg cgctgggctt tccaggtgtg gaacaggcct
    25021 atgcgattca ggcagggcga gagctccgcg ttatggtcaa tgctcgcgag acgacggacg
    25081 aaatcgcggc aaaaatctgc cgcgatattg cacgcgcctt tgaggaacga ttaacctatc
    25141 ctggagaaat caaggttacc gtcctccgcg agattcgctg tactgagatc gctcgctgag
    25201 ctattgggga aacagagtac gggccatcac aggggcggtt cgccgggttt tcgccccgta
    25261 gcccgcagcc agagaaaggt ctacttcgtg cgattgttgc tggtaggaga tgttgtcggc
    25321 aaaccgggtc gccgcatcgt cacccgggca cttcccttgc taagacgcca ttgccgactc
    25381 gattttatcg ttgtcaacgc ggaaaacgcg gctgggggat cggggctgac acccgaactt
    25441 ttctatgaaa tcgtggaggc cggggctaat tgtatcacga tgggagacca tatctattcg
    25501 cgacggcaat tggaggccgt gctccgcaat gattcacgga ttgtgcgccc tgcaaactac
    25561 ccccctgaag ccccggggcg agaattcacg atcctcacgg ccgccaacca agaaccggtg
    25621 gccgtcatca gtcttctggg tcgcgtcttc atgcgaccgg tcgattgcta cctccaggcc
    25681 gccgaccggg tccttgataa gctttcgagc cttgtcaagg ttatcctgat agattttcac
    25741 gccgaagcga ccagcgataa gcagattttg ggtcgatact tggatggtcg agtctcggcc
    25801 gtcctgggaa cgcataccca tgtccccacg gctgacgaac agattctgcc gggtggtacg
    25861 gcgttccaat gcgatgtagg gatgacaggc ccccacgaaa gtatcattgg gcgaaggatc
    25921 gaccgcgtcc tttacaccac gaggacattt gaaccgacac cttttgaggt tgctacagaa
    25981 gatgttcggc tgaacgggac gattgtcgaa gttgatccgc tgactggtaa gtcgcttggt
    26041 atttggcgac tttgccttag ggaatctgac ctcacagaaa cgatcttgtc gcaagtcgcg
    26101 aaaagcgtta tcctacctcc cgcgagaagg acggagtgaa tgcgtctccg tacggttttg
    26161 cgccaaactt cgtcggcaca gccctgtgat atgtgtagcc cacactaccc gggcgatgag
    26221 ataagggtaa ccgctcgctg gccatggaca caacgactgg aaatcggggc gctaggcctt
    26281 ggattggctg gtttattgtt cgtggcccga tggttacaac cagccgcaga aggatttgga
    26341 acgcaccagc agttggggct accgccgtgc accatggtgg tggtttttgg caaacgctgc
    26401 ccgacctgcg gtattacgac agcctgggcc tattggacgc gaggcaaggt ccgggaagcg
    26461 ttgagctcga gtgtttcggg aacgttgttg gcggtgctgg ccatggtgac ggcaccctgg
    26521 ctgataatct ccgcctatcg cgggcaatgg gcatggtggg ccccaaagcc aggcaccgtt
    26581 accttagctt ttgttgtcct atggataggt ttgctatgtg agtggttagt gagactttgg
    26641 tgcaactaga ccggcagtcg gccatgggtt aaaagacccc atagcgggat tcacggacga
    26701 ataggtagag ttagcaagga atggctatga ttgctcttat ccggaagtta ctcgctctct
    26761 gtttatgcgt gctcccttgt gtgggccttg tcggatgcag tgccctggct acagcgatgt
    26821 ggattacagg cgcttcggac gtcaaaccgg agtatgacgg cttcaaggac aagaaagtgg
    26881 cagttgtggc acgccccgtg gtcgcgctca cctaccgtga tgctcacgtc gataaagaac
    26941 tcgcgcgaag aattcagttg atcctcacca accgcgtacg aaaggcccaa tggattgacc
    27001 agcaaaaggt cgaaaagtgg ctcgacgagc atgagtggga aacctttccc gaggtgggga
    27061 aaggcgttgg cgccgacctg gtgattgggg tcgaaatcga agatttcagt ctgtacaagg
    27121 gacagacagt ttaccaagga acagccacac ttcgggtagc tgtttacgat tgcgccacga
    27181 accaaaaggt ttacgaaaag tcgctcagca atattaacta tccacccaac tcggtcgtgc
    27241 ccacatcaga ggtgccggag tcggttttcc gccgcgagtt cctttccgtc ctggcggagg
    27301 ccgtcgcgag attgttccat tcttacgacc cccacgaatt ctgggccctg gatacaagaa
    27361 ccctacgata aaaaggcagc agcgcttaat gggaattcgg cggcccgcgc ggccgcgtca
    27421 tgtcgtcatg acttgcagca ttcagagcct atcccaaaat cccctctccc tccgggagag
    27481 ggttggcgtg agggcaagcg atcgtcggag tcgatccaaa aaaccagcag cgattcgagc
    27541 caccctcacc cccggcccct ctcccgccga acgggcgagg ggagcttgtg ggataaatct
    27601 taagcacacc agggtcgctc acggcgctca ccgagcttat agtaggcgtc cttgaaaaag
    27661 gccacatcct cagcaatttg aaaatcgaac atcccgacga tcacgaaatc ggccccgttc
    27721 cgtaagacat aagggaatgc catccgtggg tgaatcgctc cggcagccag caccttgaag
    27781 gcaaaccacg gcttacgaac cgatttcatg aactcgatcg tctcttccgg atcgatgcac
    27841 cacatgttgt cgttgaaaga gaatggctca tcacgccggg gtttgaacca gccgaaatct
    27901 tcccgaagct cctcgggtgt ggccgaccaa tagttgtcgc tgtgcaaggt cttaacgtaa
    27961 aagtcggccc cgataccatt ttcttcacaa gccttgggga cactcaagga atggctgcca
    28021 acaccgccag gtacccccat ctccttgata gcctcgaccg ttttagccac tacgtcaaga
    28081 cggccattca tcatctgctg gtccgtggcg ccaccgtggg tgtaaaagaa cgtggcaccc
    28141 tgactcagta gcgactccat ttgggactta agcttacccg tatcagcatc cggattaaca
    28201 cacacaaggg cctgcatctt gcctccccgc tctgtgagat atcgatggaa gacatccatg
    28261 caccttgggt cgagctgaac agtgttgatc ccatgcgcct cgcaaagggc gaacgtttcg
    28321 aacacttttt catcggtgtt gtaggctttg aagagccgtg aaacgtagag aagatcacgg
    28381 ctgtgcgccc agcccccaat aaggtttcca ccgatcaaca tccggctgat cttaagattg
    28441 ccgatttgcc caaacggcat tggtcggtca tcttgagtcg cgggttgctg attggcccct
    28501 tgttgaagag cagccagaag gattttctcc tcaagaccga acgccgtcac acccgccgca
    28561 ccgacaagac cgcgccggag aaaatctcgc ctgtcacccc gctctgacat ggctcgtttc
    28621 ctttcaacta tgaatcaccg tctttgagag gaacaatcgc caactcgata taacgccttc
    28681 tcgactgatg ctgcccaatt ttaggacagg tcatgcccga ccgcaatgtc acaaggttcg
    28741 accatcctcg ccacgtgtga tccgtatctc cgggacaccc atcgatggtg ggtcatcttt
    28801 gcgtggagcc tcccaccact gctaaaaacc ttgttgttta ccgatagtct tcgccattca
    28861 aactgcgaag gaccgacttt tgccatgcct ccaacgcggc ccccattgtc tgaacgcgct
    28921 caggctcttg cgaggccagg tctgtcgact ctgtcacgtc cgtggccagg ttgtataact
    28981 cccatttgac agctccgccc ttgcccgcaa tccggtgtag cttgaagggc cagtccaacc
    29041 atgccgcatg accgaggagc tggtcctctg aatattgttg ggtaatttta tcctcatcct
    29101 tcggtggggg agatgccgaa gcccttccct cttgctcctc caacatttgg gcaaggatct
    29161 tttcactcgg cgtgctaatc cccccgacag ggaaaaccca aaagcccagc ggcttgtccc
    29221 gtcggggtgg ctcccgacct tcaagcaagg ggaccaagct gattccatcg agtggcggct
    29281 gattggcgat tgtgatgccc acaatgtcca aaagtgtggg atagatatcc accgtgccac
    29341 aagggaagtc actgcgtcgc ggggttttga tctttgccgg ccattcgata acacagggga
    29401 cacggattcc gccttcataa agcgtgccct ttcgcccacg aaggcccccc gtggagccaa
    29461 cctcaatggc gccgttatca ctggtgtacc acaagagtgt attctcatga attcccaact
    29521 cgcggagaaa cttccgcaaa ttacccacgg cgcggtccag ggctgtaatt tccccgtaga
    29581 aatcttgcaa acgttccggc tggtccctgt agaaggcctt atcttctggc aaggcccggt
    29641 gcgggctgtg cggcgaccca aaccacacca cagcgagaaa aggtcgccct tccttcgtcg
    29701 cttgccggat aaactcaatc gcggcctcca cagcaatgat tgaactatcc ccctgttttc
    29761 gcaccacccg gccctgatca ctcatcagcg gatcaaggtc ataaaaattg ggcgtggaaa
    29821 gccagcgatc aaagccagag gccccagggc aaagttgact atccgcgcga accggtccca
    29881 cgtgccattt accaaaatgg ccggtggtgt aaccggcccg ttttaaggct tcggcgatgg
    29941 tgatctcttg tggccgaatt gtgtgtcccc atgaaaagca accataccga ttgggatgcc
    30001 gcccggtcag cacgcttccg cgggttggcg aacacacggg tgcggccgca taaaagtaat
    30061 cgagccggag accctgcgct gccatctcat cgagatttgg tgttttcaat acaggatgac
    30121 cgttgtaacc tacgtcgccc catccctgat cgtccgccat gcacaggatg atgttcggct
    30181 tttgtgatga ctcagcccct aggataggcg tcttagaaaa tctagaggtc accagcccca
    30241 ggaaaattaa acaaaaaaga atacctcgtt ttgccaacca acagccgttc atcgtcacat
    30301 actcccatcg ttgtgaatat gtctagtggg cctcgctggg tcactctgcc atgaggaaca
    30361 aagcatccct ggctggcaga acaaccatgt cacccacggc tgcgccgtta ttggtcacag
    30421 ggtcctgttc gggactgccc ttgattctcc gaaatttgcg gcctggtgca atagtcctca
    30481 gatcatagga aacctcatgc aaggatggat tggccaacac caggccgtgc tggaatacac
    30541 ggtagcgggc atcggcggcg ggataaaggg ctagcctttc cacacgaact ggctccgcac
    30601 cttcgacacg caaacggaga tgataattcc ccggttttag ctcgggaaaa taaaagccag
    30661 cagaaaacgg tttgcgacca acccacgtcc cctgctcgcc gaccgagccg accaacgtgc
    30721 gcgtgtcttg agtttccgtg gggacaaggc tacacgtcgc atacctcgcc atgttctccg
    30781 gatagcctcg gcggggttga gcactggcaa cacactcgat gtaggcctcg cgggccgaaa
    30841 tcgttatagg tatctcgaac acaacatcct ttccctcctc atcggcctcg accacaaacc
    30901 ctgattcctc ccaacgagtt ctggccccca atacgccaaa gtttgcgttc ttttgtttca
    30961 attgattcag cacaggagca agaaggtcgg gtgcctgggt tgccagcgtc tgggtaggtg
    31021 ccaggggccg accaagccag cccaaacgtc tctcctgtcc tgcccaaaac tcgtcccata
    31081 cgccgatcac agaggatgga tcttttggcg gagagaaaga ataacagatg gccgcgtccg
    31141 tcatgacggc agccgcaaat actaggcgat gtattgaaaa cggcacgtcc ggcgtggcct
    31201 cgacacccgg ctgcgtgccc ggtcgcacat atttatggtt gatatagtta aacaccggag
    31261 gtcgcccaaa ctgagcccaa aagttgtgtc tattgatccc ggtggaccac tgtttcattt
    31321 cccagtcgcg caaccacgga aaaccttcac tttcgatgcc gttgacaagg ccaaatcctc
    31381 gctgtgtgcg tgcgtctagt tggccatcgg ccaggatcag cttatcttcg ccgagccgtg
    31441 cccgcagagc ttccaggaag cgaatcaccc cgatgccgta cacattgacg ccttcaatca
    31501 caccgccgtc ggctttgcca tcggcatcgc aatcgactgg acggcgtcct gctctcgggg
    31561 agtgatggag cacatcgaac tccagcccgt caaacagagc caattcgcca cctggaccaa
    31621 atcgccgccc caattcagca acgaggatgt ctgaacaggt tctgccgttt ttgtccttgg
    31681 gacagcacgt ggcatagttg tagtaccaga gtaagtggga gttgcgtccc cacggacctt
    31741 cggtagcatg cgccgccgca taggcacgcc cggccgcaaa gctccgcggc ttggttccgt
    31801 aacaaccccg tttcacaaga atggtcccct tatcccgatt gacagctaga agttggacct
    31861 gctcgctgcg attccagtca gggcgacctg agtcatccaa ttcgcatagc ccaatatcgt
    31921 cgttgctgtt tcggtaccgg ccgatcccag tcaagtagca acttggatcc tctactttga
    31981 tttcggttgt accttcttcc gccggcacgt cggagagaat ctttgagccg acatgataga
    32041 tccagtgtcc ggcaaagtat tcctccgttt cgaaccgagg atcccgcgca ttaccattga
    32101 agtgcaacaa gaccaattgg tccggataac ggcgcttgaa gttgacaaaa aattcgatgt
    32161 tgttcgccga ggtccccggc acttcctcat cgagcacctt tccttctatt cccataagac
    32221 gtgaaaaagc cttctcccag gcatcaaagc tcaatcgccc ctgccgcgcc aagccttcac
    32281 tctggcgaaa gaaaaagacc ctcgggtaat ccccacttac aacggggagc gatgactgag
    32341 ccagcaacga ttgctctgag cttgcgctaa acacttcgca aaaaagcagg ccagccgtca
    32401 cgacaagcaa aaacctgtgc cttttcattt cagccctccc gacagtattt cgacgccaca
    32461 ggaatgcgaa catacaaccc aacctgcaat cctaatgccg attcacgggt tcggcgcacg
    32521 tcaacgccac acttgcgaca cccattcggc ggcgacggag cgcggccctc gaccgccatt
    32581 tgggcggtag gccatccccc gcgtccgctg tatcgtaccg ttggtttgcc gtattggttg
    32641 gcgacagcgg cagtttccgt aacgcacgca ttaggcgcgc aatgacgagc taataagcaa
    32701 agcgaaaagt tgcgtaaaga ttcggtattg aaaagctgga ccagcagtta ccaaacgctc
    32761 tcgaaatgtt cgcccattag cggacgattt gcgttccggc aacaatttcc atcatccacc
    32821 ggttgacgtc ggctaacata tagccggaaa gctccgagca gcccgggtat tgacgcagca
    32881 tcacattgat gcccgccgca tgcaagaact tgaggtcctc ggcggcctga cacggaccgt
    32941 aacaatcaca atcccggccg atagtcaaca agaccggcaa acgacggatc ttggcaaact
    33001 gggctagcgg gcgatgtgtt ctcgcaatgg gcccacacaa tgaaactgcc ccggcaaaaa
    33061 gatcagggta acgccaggcc aaacggatgg ccatcgttcc ccccgattgg tgacccgcga
    33121 tgaaaacctt gtcccgcgca atgcggaatc gcttttccgc ggatgtcatg gcatggaaca
    33181 cacaactggc cgcctgaacg ataccttctt ccgtctgcgg ccagttggaa ccgaaatcct
    33241 catttgagcc cacaccccgc ggtgcgactg caacgtaata ttggaggtct aattggggca
    33301 tcacttccag caattggcgt tcgccctcac tttcttcacc atggagccaa accaacagtg
    33361 gataactgta gcgcggctca taatgcagag gggctactaa gacacgtgga tgtgatggaa
    33421 gcgtttctgt tacacggtca ccgcttgttc ctgctatcgt tggtgcttga tcagagtccg
    33481 aaccagaaaa gcgctcacat tcactcatta tcacaaccac cttgtcaggg gaccgattga
    33541 tacttccggc aacactgttc ccaccgcagc aggaacaact ccaccctcac aagctccccg
    33601 ttaaacgaga ggccgtcaca aggtaacccg tggcctcaaa ccggggtgcg aataaaaact
    33661 gtactgcgat tcaccattcg tgtcaatcgt gatctgaaga gcacataaac gaccatggta
    33721 aacaacacag ccacgcaaaa ccaaacccac ttgatcaacc aattcttcaa cgcgagatac
    33781 cgcgggtcgc ggcatatcgg gtcccacgtt gtccccgttt acacaactta cccacgttta
    33841 ccagcacctt gtcatcgcaa taatctattc atggtttgaa caagattctt gaccgcctca
    33901 acgctcttct caaaagccgc tcgttcttcc ggagtcagct ctaactcgat gattttctcg
    33961 acgccatcct tgcccaggat gactggtaca ccgatgtagt aaccaccgac accgtactcg
    34021 cggtcacagt aggcggcaca tggcagcagc cgctttttgt ccttgatgat cgcctcaann
    34081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cgggcctctt gtcgcgcaca
    34201 tcggaagatg ttacgtaaaa aggtttggga atcgtcgaag cgtggccaat tcctcgtaat
    34261 ttgtcgaatc agtaaacaag ttcggcttgg agcaacgcga gaaaaggagt cgggaagatg
    34321 gatatccagc aactcgtttc ggagctaaaa gagaaacgcg gtgggcgaat catcatgatg
    34381 gtggccgacg gccttggcgg cttgcctttg tcgccgggag gtcccaccga gttggaggcg
    34441 gcgaagacac cgaatctcga ccgcctggcc tcccgcggcg tgtgcggact tatgacgccc
    34501 attaagcctg ggatcacacc gggaagtgga ccgggacacc tgggactgtt tgggtacgat
    34561 ccgcagaaat atgtcatcgg acgcggtgtt ctagaagcca ccggaatcgg tttcgccgtt
    34621 ggtcccaaag acgtggccat ccgatgcaat ttctgtactc tcgatgccga aggccgcatc
    34681 accgatcgtc gggcgggccg catcccaagc gaggaaagtg ccgccgtcgt tagcctgctg
    34741 aaggccatcc gcattccagg ggtcgaagtc tttgtcgaac ccgtgaaaga acaccgtttt
    34801 gtggtggtct tccgaggcga aggccttggg gccaatgtgg ccgacaccga tccgcaggtc
    34861 gtcggcgtgc cgccactaga ccctaagccg ctagacgaag aaagcaaaag aacggccgaa
    34921 atcgcaaagc agttcgtgac cgaagcccgt aagatcctgg caaatcagcc aaaagccaat
    34981 ggcctgacga tgcgtggttt ctccaaacgg cctgatttgc ccacctatgc cgaagcctat
    35041 ggcctgaaag ccgccgccat agccgtttat ccgatgtata agggcctagc cagcctcgtc
    35101 ggaatggacc tgctcggatc acccacaacc ctcgatgagg agatcaccac tctggagcag
    35161 gcatggcggg agacggacta cgatttcttc tttgtccact ttaagtacac agatagccga
    35221 ggggaggacg gcgatttcga tgccaaggtg aaaatgatcg aacagtttga cgccgtcatg
    35281 ccgcgtgtcg aggccttgcg ccccgacgtc ctgatcgtta ccggcgacca tagcacgcct
    35341 gccaagctca aaagccatag ctggcaccca gttcccaccc ttcttgttgc agatacgtgt
    35401 cgaccggacg gggcgaaaga attcagtgaa cgggcgtgcc tctannnnnn nnnnnnnnnn
    35461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35521 nnnnnnnnnn nnnnnnnnnn nnnnccgcgc gtcgaggagc taattatcgg acaagctgag
    35581 ttctgcgccg ccatcttgcc cgggttgccg aggcgacaga tcatcgctcc tttgccgtcg
    35641 gtactcagac gagccgcatc tttccgaaag aaacatcgcc atccgagatc aaatgtttac
    35701 aaccaagtta tctgtggcaa taaccgtgtt tgagaagata cgggccacct cgtcctgttc
    35761 tttttcacca tcacaatcca cgatcggatt aaggtgcacg atgaccaatc gctttgccct
    35821 cacctggcgg gcaacctcgg cagcttgcgc aaccgtcacg tggccagttc tttgcgccat
    35881 ctcctcatac cctgccggat aaaacgcctc atggatgagc agatcaacat cttgaatgac
    35941 ctggccatag acgggcaggg cttccaaagt tgtatccgta atgtaggcca ccgatcgatc
    36001 acctatctga aaacgataac ccagtgcccc tccaggatga gaaagcggca ccggaaggac
    36061 agaaaccgac tcacatcccg cccgctctgt ggctattgca acgctctcgt tcggactaac
    36121 cggaataaac tcgtacggcg gtatcttggg aaaaagcggc tgcgagaaaa gtagatcttt
    36181 gatagccttg agcgtgctct cgtccgccca gatcttcaca gggcctacct ctttttgctg
    36241 ggcaaggccc cagtagtacg tcaagccaac cacgtgatca agatgggcgt gtgtcaaaaa
    36301 gacatgccag ggtttgtggt ccaagaaacg gccgagtcgg aagaaggcag ttccggcatc
    36361 aaggattaca ccggcctgtg gaatcacact gcagagcgtg tgcctcttgg aacttggatg
    36421 gtaaccgccg ctacctagga tgtgaatttc cataattcac ccctatgagg atgccttatc
    36481 aatcaatgtc tcgccttttc gcatcgcgcc accagccgaa gcaactctcg tgtctttggc
    36541 gattctcggg tcccatcggg catcggtgcc acagccgggc acaacccttc ggccactctt
    36601 ggcgacgatc agaacgccca ttatcccccg gggacaacga tgtatcccaa atcacgtctc
    36661 gtcaactttc gttattcttt ccaaggtagc accccaatga tgacgatggt agccacaagc
    36721 atcaaacgtt aaccggggac ctgttactgc ggttccgcta cccgataaaa taagacaaaa
    36781 cggcttggaa ttgacgggat gaggccattc tgagtaagag gcataagact aaatcgcgaa
    36841 ttggccaaga ctatacgata aaggattcgt tttgggcccg tagctcagcg gtgagagcag
    36901 gggactcata atcccttggt cgggggttcg aatccctccg ggccta
//
LOCUS       DAOSUT010000017        36574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_803_length_36574_cov_9.662778, whole genome shotgun sequence.
ACCESSION   DAOSUT010000017 DAOSUT010000000
VERSION     DAOSUT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 36574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36574
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_36574_cov_9.662778"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1731)
                     /gene="der"
                     /locus_tag="PLD05_02685"
     CDS             complement(247..1731)
                     /gene="der"
                     /locus_tag="PLD05_02685"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOP76365.1"
                     /translation="MCPVAGFVPNGVFAKSGFGEEMAIPKVAIVGRPNVGKSSLLNWL
                     VGRRVSVVDARAGVTRDRVTALAKLDERLVELIDTGGIGLVDVDQLEDHVTRQIEIAL
                     HEADLILFVVDTRAGLTALDEEISRRLHTLQRPVICVPNKTDAPSLEPAAQEFSSLGW
                     PIVPVSAEQRRGKSELITAMLDLLPQELSEDDKAAIEPEMKIAVVGRRNVGKSTFINT
                     LAREERMIVSEIPGTTRDSVDVRFEMDGRVFVAIDTPGLRRRKSVRENVDFYGVCRAE
                     ASVRRADVVLLFFDATQDISGVDKQLAAYIAEQYKPCIFVVNKWDLMVGQIPTDVWVD
                     RLAKAFGTMSYVPVAFITGKTGKNVKRLIELAQSLFKQSRQRVSTGRLNRVLQRLLKS
                     NPPPLVGTKQVKIYYGTQVAVQPPTIVLVCNEPSLISSSYQRYLLNAMREELPYPEVP
                     IRLLLRKRESRRVASKIPALDERDEEPSDDDVWAEDWFESTEET"
     gene            complement(2157..3713)
                     /gene="trpE"
                     /locus_tag="PLD05_02690"
     CDS             complement(2157..3713)
                     /gene="trpE"
                     /locus_tag="PLD05_02690"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOP76366.1"
                     /translation="MSRKRYYPDFESFQQLAEGFDLVPVYRRLVSDSLTPVTAFHKLD
                     AGRCACLAESVTGGEKVGRYSFLATHPFLEIEAWERRVIVNSYTGVSASGAPLLETRQ
                     FISDNPLGELRKRLEGLRVRTLPELPPFCGGAVGYVGYDVVRYYERLPNPPPDDRNIP
                     DLSFGFYDRMVVFDHVTKSLIVVALAHLDRPGWTAEDAYADACRRIDEAVDQLTHSPC
                     ELEPFDICVETEPCVEFTSNFTREDFEEAVRKCIRYIEAGDIFQVVISQRLEVPLQAH
                     PFEVYRALRVINPSPFMFYVRSPSVTLVGSSPEILVRVVNRQVTTRPLAGTRPRGKTE
                     EEDRRLAEELLADPKERAEHVMLVDLGRNDLGRVCRYGSIRLTDVMTVERYSHVMHIT
                     SNVTGELAPGKTAFDALQACLPAGTVSGAPKVRAMEIIDELEPHRRGPYAGAVGYFDF
                     SGNMDTCITLRTIVVQGGKAYVQAGAGIVADSDPHREWQETMNKARGLLKAIEVAQQR
                     RPAFQVASVR"
     gene            complement(3907..5163)
                     /locus_tag="PLD05_02695"
     CDS             complement(3907..5163)
                     /locus_tag="PLD05_02695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOP76367.1"
                     /translation="MTTRHETAILDPQLYRPDFPILNRTIEDGRPFVYFDNAATTQRP
                     RQVIEAITTLYETHYGNVHRGIHRLADETTELYEGARERIARFLNASSETEVIFTSGA
                     TAGINLVARAWGDANVHEGDEILVTELEHHSNLVPWFQLAERTGAVIRYIPITDDGLL
                     VMEELERLLSSRTKLVAVTAASNVLGTVPPLPVIIKAAHSVGALVLVDGAQGVPHRKT
                     DVQALDADFLVFSGHKMLGPSGVGVLYGKREILESMPPFMGGGSMIREVRMDGFTPGD
                     VPYRFEAGTPPIVPAIGMTAAIDYLEQVGLERIDQYERQLTKRLHEELEKLEFVRILG
                     PTPEYKSGIVSFVVDRIHAHDVAQVLDSVGVAVRAGHHCAMPLHKRLGVTASVRASFY
                     FYNMASEIDRLIEGLDRAVRLFRRRK"
     gene            complement(5672..6097)
                     /locus_tag="PLD05_02700"
     CDS             complement(5672..6097)
                     /locus_tag="PLD05_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOP76368.1"
                     /translation="MDQDEIYQERILEHYESPYHKGRCSECTHCHEDKNPLCGDVVRM
                     ELRIIDGGKIREIYFDGEGCCISQAAASMLVELFDGKTVEDVKKFTAQDMLKYFGPRL
                     TPNRQKCALLPWRVLQAAIYSPCPQVPPIPEAPKESVAK"
     gene            6292..>7385
                     /locus_tag="PLD05_02705"
     CDS             6292..>7385
                     /locus_tag="PLD05_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOP76369.1"
                     /translation="MFQLVSPFEPAGDQPKAIKALCEGLDKGKRFQVLMGVTGSGKTF
                     TMANVIQHAQRPTLVMSHNKTLAAQLYAEFREFFPYNAVHYFVSYYDYYQPEAYIPQR
                     DIYIEKDASINEQIDRLRLAATSALMTRRDVIIVASVSCIYGLGSPEDYRTMTVPVIK
                     GQVLDRDEFLLKLVNIQYERNDVEFQPGRFRARGDCVDVFPSYEPFAYRIEFWGDEVD
                     QIASIHPVTGEVLQRHDQVFIYPAKHFVLPESRIQAAIVSIREELEQRVAELKSQGKI
                     LEAHRLSARTRYDLEMLQEVGYCPGIENYSRHLAGRPPGSPPDTLFSFFPDDFLLFVD
                     ESHVTIPQIRGMFGGDFSRKQTLVEHGFRLP"
     assembly_gap    7386..7485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7486..8106
                     /locus_tag="PLD05_02710"
     CDS             <7486..8106
                     /locus_tag="PLD05_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOP76370.1"
                     /translation="DLREGAFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSETS
                     LIQTIGRCARNVNAKVILYADKMTDSMQKAVEETRRRRKLQEEYNKAHGITPETIKKN
                     IRAGIEAEVAAHNQANALVGRSDEAQYITEEYIAELEAEMLAAAEALEFERAAAIRDR
                     IQLLRKQIGKPVTEAQLKSPQKRRRGGRRNTIHPSPGIDITQFRRK"
     gene            complement(8277..8486)
                     /locus_tag="PLD05_02715"
     CDS             complement(8277..8486)
                     /locus_tag="PLD05_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76371.1"
                     /translation="MKKLGSLLIVLALSSLFVGCPKQAPAPAGGAPAPAASSESAPAS
                     DESAQPAEQSQEAPAEQGTGGESQQ"
     gene            8689..9198
                     /locus_tag="PLD05_02720"
     CDS             8689..9198
                     /locus_tag="PLD05_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP76372.1"
                     /translation="MDEGSRQPTIEELVVQHAQTVFRYAYRLSGSVADAEDLTQETFL
                     LAAKNIAQLEHPARAQGWLLAITRNAFLRQRRGPHVTLLDDMAATIVESQLDSDDTDE
                     QLIQIALQSLPEQQRVMILMFYFEDMSYKDIARELGVPIGTVMSRLARAKAALRKKLD
                     ALTKKVAPV"
     gene            9233..10135
                     /locus_tag="PLD05_02725"
     CDS             9233..10135
                     /locus_tag="PLD05_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76373.1"
                     /translation="MASQPIDPKDEDLLEIIQLDRDDPQDAFAQRLLAILRENPALAK
                     RYEEIRRRDAEIGNRLKQGNKLSGLEERILARLGYQNEATDNHLGSSQTLDVAHPESG
                     ALRSSLCEGGASPVVTSRVVTGAPVPRVGRRQWLRWVGALATAGAVGGLGIWVWQRRK
                     EKPRISALALGNAATELFEATLDGFGTGRLLGRENPPQGYRLSRDVQVFRGTVITWRQ
                     VTLADLPAIVFDLSHPGGEKGSLFVIRLVLPDLPPMPPRRPCLRTSRSSAGWWQDNDL
                     ACVLVVLGGPRVYERFLAPPAPLT"
     gene            complement(10243..11208)
                     /locus_tag="PLD05_02730"
     CDS             complement(10243..11208)
                     /locus_tag="PLD05_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOP76374.1"
                     /translation="MMKLTRRGWFAGVGALVGGWLSGSGVATWAEERSSNSSLSVDGF
                     RFVHMTDLHIQPELKAAQGVAACLHHIQSLNPKPDFILTGGDLIMDALQQGEERASML
                     FDLLKKVFRDHNSLPVYHCMGNHDVFGWGKKQGVTEEHPKYGKKMYMEKMELERPYYA
                     FDHKGWRFFVLDSVQPSETELYQGGIDGLQMDWFRQELARKPKEMPAAIVTHIPFITV
                     TVLPDSAKEGRFQIGSASMCAGAQQMAQLLADNNVKLALSGHMHMVDEVSYRGITFAC
                     SGAVSGRWWKGVHKGFPEGYAIIDISPANQISYQYIPFGWQAVES"
     gene            complement(11351..12223)
                     /locus_tag="PLD05_02735"
     CDS             complement(11351..12223)
                     /locus_tag="PLD05_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOP76375.1"
                     /translation="MIATFEYIEQIVATCRNAARANEETPARRGNLIVLTPEDGSDVI
                     ITGDIHGNRRNFNLIRRVAALDKNPKRHLILQEVCHGGPTYPANAGCMSHTVLEDVAK
                     LKVQYPDRVHFILGNHELSEVTDYPIQKNKQMLNLLFRLGLQHVYGPAADRVRESYLA
                     FIKSCPLGVRLPGDLLVTHSLPEAVDRQGFDVTVFHRPLDMVEFLEHKSVFRLVWGRD
                     YRRENAMAFARLTQAAVLVHGHEPCPQGFATPNDVQLILDCSSDKAAYVMLPLDRQTP
                     WTLDEVVARVQLLA"
     gene            complement(12388..12876)
                     /gene="sixA"
                     /locus_tag="PLD05_02740"
     CDS             complement(12388..12876)
                     /gene="sixA"
                     /locus_tag="PLD05_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOP76376.1"
                     /translation="MNVYIIRHAWAEEPNEARWPDDSLRPLTKEGRAKFRRVARRLGR
                     FGVAPRVVVTSPFLRCVETAEILVSSWQEPMVLETVPFLAPQGDIHQLIEWTRTRMGQ
                     LSGDIAWVGHAPDVSKILATLIGGGTFHFAKAGIAAVQFKGPIAAGEGTLLFLIPPSL
                     LL"
     gene            complement(12980..14167)
                     /locus_tag="PLD05_02745"
     CDS             complement(12980..14167)
                     /locus_tag="PLD05_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOP76377.1"
                     /translation="MSRFSWRLLRRVSPRLLLKAGRLWLIPGLRSLSRFRKRVAASEL
                     FPPFFFLSLTDACNLRCHGCWITSQGQPRQLSRDDVNHFIAQAKVHGNHFFVLLGGEP
                     FLYKDLWTILRQNRDCYFQIITNALLLDEAAVATAKEVGNATILVSLDGFESANDARR
                     GQGTFQAIKEAFQRLRKGKILFGVATTITSENYEAALSPEYVNWVDEQGAIYLWYYIY
                     RPVGPDPMPQLALSRADILKVRRQLLRLRRISPILIVDTYWDADGKAVCPAALGLGYH
                     IGPAGDIELCPPLSFARETIRDHGGDVFHTINTSDFLRGFQKFALSRTRGCPILECPH
                     ELEAFLREHHARDTSGRDAFAELRASSPRASHHIPGEELPETMGTYRWLKKHLFFGLG
                     AYG"
     gene            complement(14365..15606)
                     /locus_tag="PLD05_02750"
     CDS             complement(14365..15606)
                     /locus_tag="PLD05_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP76378.1"
                     /translation="MTPKQRVLAALEHHEPDRIPIDFGGHRSSGIAAIAYARLRKYLG
                     LPERPIRVYDPIQQLAIIDEDVLNRFRVDTIELGRAFAQEDRYWVDWVLPDGTPCQMP
                     SWAVPERSDGGWVLRGPTGRILGRMPDGALYFEQCYWPFAEKDQPERAASALIDTIWC
                     GIPSPPGPIVEGPGGLDRLREGARRLRQQTDRAILFLFGGNLLECGQFLYRNDNFYML
                     LAGDRPRAEKVLDSLVEAHLANLERVLPVLDGLIDIIVFGDDLGMQSGPQISPKMYRE
                     LFKPRHAAMWKKAKELADVKVMLHCCGGVRELLDDLIDAGLDAINPVQISCKGMNPTE
                     LKRDFGKRITFWGGGCDTHRVLSFGTPEEVKQHVREQIESFAPGGGFVFQQVHNILAN
                     VPPENIVAMFDAVYEFGAMYH"
     gene            complement(15897..16841)
                     /locus_tag="PLD05_02755"
     CDS             complement(15897..16841)
                     /locus_tag="PLD05_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOP76379.1"
                     /translation="MHRVALLGHFIVYVIVRFLIFVVQMIPLAVGSWLAWCLAYLFTY
                     VLRIRYSVADENLRYAFAELSPSQRRDLILRMWHHLFLLVMEVAHTQAKIREENWRQR
                     ATLKNVEPLVKLLLEDRPVIIVTAHFGNFELGGYMLGLLGFPSATIARNLDNPFLDRF
                     VQRFRAARGQYLIPKVGGADQVEEMIQKGHTIAVLADQDAGRKGCFVPFFGRPASTFK
                     AIALLSLEYDAPIAVVYARRTGKPLHFEMGLVGILDPRDVSAPDAVTFVTQWYTSRLE
                     EMIRSAPEQYWWIHRRWKTQPKSPKLRIPSEQAVRSAA"
     gene            17126..18613
                     /locus_tag="PLD05_02760"
     CDS             17126..18613
                     /locus_tag="PLD05_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76380.1"
                     /translation="MFARWGGVLLAVWSLGIVFVGWPQGLACEPGPELRTSTPSVERP
                     NLLVIITDQQHAGMLSCAGNPYLRTPAMDSLAAQGARFELAYTANPVCIPARMSMVTG
                     RMPSAFQIRSNAEGKNPAPKEAIDQAMGWIFRRAGYKTFYGGKTHWMRGMTPQSTGFG
                     ELTKDTREELARKCAEFLSEPPAEPFLLVASFINPHDICLMAIDAFTQATGRPAIYPE
                     LQRDRECLAEALALPAGISEAEFFDRLCPPLPNNFEIPSNEPDGVTLSYLPPQSFRAY
                     VRQHWTERDWRLHRWAYCRLTERVDAEIGIVLDALRQHGLDRKTVVIFTSDHGDLDAA
                     HRLEHKSIPYEEAVRVPFIVAWPGVTKPGLVDRTHLVSVGLDLIPTLCDFAGIEKPPH
                     LLGLSVRPLAEGRTVDTWRESVVVESDAARMIRTARYKYVVYEKGTRREQLIDLKNDP
                     GEMENLAGQSAYDQILKEHRQLLWEWVTRIGDTIGQSFVIAPDER"
     gene            18718..20781
                     /locus_tag="PLD05_02765"
     CDS             18718..20781
                     /locus_tag="PLD05_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP76381.1"
                     /translation="MSRLGHTPDWSRRKFLTASTAGLAGLAIPVRSASARYDPPVHPQ
                     FQRLSQNLGLLRGTIHVGVVFNGNEALLIDCGDVGTFEWAAAAGIKVNKVLVTHHHRE
                     QVMGLLSEETNAEVLVSAAEEKYFTDVAQYWLAPSSRWHIYNQRPHHWMLPQSVKVAG
                     VLKGGESLTWGSATIKVLETPGHTDGSVSFLVAVDGLRVVFCGDLLYGPGQVPDLYCL
                     QKGLHRGQRRTSDYHGFLGARETVKESLHRVLGQQPDLVVTSAGGVIEKPEVGLQLLI
                     ERLDSLYDTYVSTSALRHYFPELFEEFQGRADHLPFRPQLAPPAYLRHIGTSWIIRSQ
                     DGAAFVMDCGSPRVIEALEKMITAGEITRVEGLWITHYHDDHVDAVPQFLEKFACPCY
                     AVRPVAEIITRPMAWRLPCISPSVVRVDRVLADGESFSWHEFRMTAFNFPGQTLYHGA
                     LFAENATDKLLFVGDSFTPAGIDDYCIQNRNFLHDGSGFLRCLDLISQLKPTHMFNCH
                     VDSAFTFRPEDCQAIKERLQQRRELLKAILPWRDFHFGTDELWVRCDPYEQTASPGDK
                     AVLDLVILNHLTEALDVHVRVVPPASWSGERLPSALRSWQDWQQVSIAGRKEGRISQE
                     IFVPKSTAPGRYVVMVDIAIPGETPSPRILCGVTEAVIVIQDVSTKTLFHNRPSPVRR
                     ERGRG"
     gene            21290..21739
                     /locus_tag="PLD05_02770"
     CDS             21290..21739
                     /locus_tag="PLD05_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76382.1"
                     /translation="MTWLKYVFGVTILLGMTAPVWAQNQEDKSQTPSVTSSGSGVLSP
                     AQQRAEWFKTMAAIAEEQAKPQPDQQKIAALQQKLFQLRQQGFAPGWQPGIGFQAPPT
                     MMRPCPWGGPGLGMGMGRGPGPGGPRGPGYGMGAPMGRGYRGGPRMW"
     gene            21920..23293
                     /locus_tag="PLD05_02775"
     CDS             21920..23293
                     /locus_tag="PLD05_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOP76383.1"
                     /translation="MPKISQVIAVFILIAVAVMFSLWQWYEYQADVAAAREVLHGHAE
                     GLCRAMVGAIRSHRRLGAFFDQQIQIILDELVDSPDVLAVQIADSAKTPIFRSGSSDV
                     LTLSVGGSFADVGYVYSASFQLAPLAAAGPQGGARGFGWGRWARGVAPEPDTSPFASG
                     GLFYATVVLDRKGFDNHVRRIARSRAGLCLTGWIALLLFGLTFRSSVRMVKADHAKRL
                     AELQARHFEELSQAASGLAHETRNPLGLIRGRAQRIAHREDPTSPTATDARLIVEECD
                     RLTARLTQFLLFAKPFTLQPVEVDADVVIEEVLLLLEPDCQSKDVRCVHHKSPQSVPI
                     VADRELTRQALFNLLHNAVKYTRPHSEITVISRVDGSNVIIEIIDQGPGVSPEHIPKL
                     FMPYFSTDSAGTGLGLSLVRKIAVAHGWDISYCPSPEGGAMFRLTIPLKTTGSGTHIG
                     EFGHTRR"
     gene            23314..24687
                     /locus_tag="PLD05_02780"
     CDS             23314..24687
                     /locus_tag="PLD05_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP76384.1"
                     /translation="MSPKTSDRLPTILIVDDERSHRALFRDLITNLGFAVEEASQAEE
                     ALEIVSRHPPQLILLDVRLPGQSGLELLPVLKEKVPTVPIILITAYGDVREAVAAMKI
                     GAVDYLLKPVDLEELEAIILDYLGCETASAPRPAVSLPPLGDVVCVSLGFRRVLETAY
                     LAARSDIPILILGPSGSGKEVVARLIQQWSHRKDGPFIVANCAGLPESIVESELFGHT
                     KGAFTGAQTERLGYFRAADGGTLFLDEIGDLPPPVQAKLLRALETGEIVPVGSERPIR
                     VDFRLLAATNRDLTTLVREGRFREDLLFRINAVELVVPPLKDRVEDILPLAEFFAKRF
                     TKRVVRFSPQAEACLLAYSWPGNVRELRNAVQRACLLSQGEIILPEHLPPSVALTSGI
                     TSDRQSTADEGRLSAMERATILATLAECRGNRTQAAKKLGISRRALIYKIRAIESQLN
                     HSSRDKD"
     gene            complement(24799..>25221)
                     /locus_tag="PLD05_02785"
     CDS             complement(24799..>25221)
                     /locus_tag="PLD05_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76385.1"
                     /translation="ENSKEYNDALISNDTWTGKIIAKLKELGLYDKTLVYVTADHGFN
                     EGETRHSYAPYVILATNDPLVKRDGMRQDIAPTILARFGLDLSRFQPPLDGEPLTQPA
                     TKPVLKAPETPPARPKAQSRQPGQRKLQKQAAPVSQAP"
     assembly_gap    25222..25321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25322..25887)
                     /locus_tag="PLD05_02790"
     CDS             complement(<25322..25887)
                     /locus_tag="PLD05_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOP76386.1"
                     /translation="MLRKIVLALSLVLLAFCPVLAQEKASAAKPRNIILIGWDGAQRA
                     HVMECLERGELPNLKQLSQEGALVNIDVVTGATDTKAGWTQILTGYNPEVTGVYSNGR
                     YRDVPKGLSIFERLKEHFGPDFVCVAVIGKREHCGEIREPFKRQLTDEEAAKIQAQQE
                     AAAKAKQAQGAKKQAAQPGAAQAKPAVPA"
     gene            26212..27324
                     /gene="speY"
                     /locus_tag="PLD05_02795"
     CDS             26212..27324
                     /gene="speY"
                     /locus_tag="PLD05_02795"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOP76387.1"
                     /translation="MQDNCGNKKDPLHDKLLGGRRILPPHVGSCETVADLIDRVFLAY
                     NAARLREAAQLFTRKMLNDDVTIGMSLAGALTPAGLGMSCVVPLIEAGFVDWIVATGA
                     NLYHDLHFAYNYKLVAGDFRLDDRQLHEVGVVRIYDVLLSLDECLIETDRRVQEMLSQ
                     PEFQRSMTTAELHYLMGRYAAEAEEKTGVGHVSILAAAYRCGVPIYTSSPGDSTIGMV
                     AAEVRLRGSQLVLDPLADVNETAAIYYWSKQEDRKSAVVIFGGGSPKNFLLQTAPHVN
                     DISGLETPGQDYFLQFTDARPDTGGLSGATPQEAVSWGKVDPDHVPDTVVCYIDSTVA
                     MPLLTAYALARHAPRPLKRLYDRRQELLDNLQKAIK"
     gene            27382..28617
                     /locus_tag="PLD05_02800"
     CDS             27382..28617
                     /locus_tag="PLD05_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP76388.1"
                     /translation="MGGKRRIKVHLTRLGQYYAILAAGIFLAAMVRQVNLLLLLAGLV
                     AAPLVFSVFLARRNLLAIKVRRRLPHRISAGDLLVVEIEIERTARRGHNWLLTLTDQV
                     IRMGEPDNDPRNRQKLSPQVTFFFLCPGRPQVASYRGRLVERGQYVFGPIRVTTRFPL
                     GLVEASLEVPIRQTLLVYPRLGILNFPAFQRSHFNHEGQTRLERRSDRASGDFYGLRQ
                     WRPGDSPRLVHWRSSLRHNDLLVRQFDRPRHRDVVIGLILRTPDDPSPGDLERVEKAI
                     RFVATLVHDLCRQGSHHIALGIAGKKTEWVIGPSSPGFRDRALELLALADANPQNRVD
                     SFMADIRRRASPESDVITVTSYEAEPLEDQILETIPSIKGEAGRQESESMRFVSSRGW
                     KGTYTVGTKEFDLIFLDQF"
     gene            28646..31066
                     /locus_tag="PLD05_02805"
     CDS             28646..31066
                     /locus_tag="PLD05_02805"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOP76389.1"
                     /translation="MALSSVRRLELSLQFSVACMATLGTTLLGLGQHSQLLPALALVS
                     SIVSFVFTDLLRVLVLRQWMTNLLMIIALSVVAVDLARASGFVNVLAMADLLVYLQCI
                     LQFQTKNTRVFRYLVTLSAFQVVSASAFYHGVLFGTLLIVYLLNSLIVMVMLAFYEVW
                     QKQMEHHKEGSQPQTVSHRLVGDRIPDSSPLGFNGRLLARTLFLGVGSLAFALVVFMV
                     VPRLSSRPWPGLSPAPLRTVGFDRQIALGELGFLVEDSTPVMRVEVTDMDGNPATVMA
                     PVYLRGGSYTFYRNGQWETNQFIVGGEVQNLKPLYRGPGQSLHRQRIILEPLDTSDVF
                     GIWPYGRWDDAPLVYDPLLTRLFRLSDRPSRISYEMTTSAIENERLVDIVPATEPVAC
                     EQYLQLPWVRVVPTWLGPRFEVDRTVYELRGWDRVVKLARQWAAESPWPANDHFHTAR
                     FFEQQLAYSGYYRYSLAGVTRNATLDPVVDFLTEHPEGHCEYFATALALLLRCQGIPT
                     RIVIGYKTYDYNTLGHFYQVRQRDAHAWVEVYLAPNQITQDLVGERPLELFRFGAWLR
                     LDPMPASALGLASSQGPWQRFWNAYNFLDYIWLKYVMQMDQPRQETEIYRPVRERVRK
                     QVDWFLDLRWWRQQVTLVVEQLREILANPLRITVLAIGLGFLALLVWKVVRILRNPTW
                     SLWLANRLPRRLRDSQLSEHLATAVYRHLEHLLSRQGYRRATGQTPRQFAGSVIQAAR
                     ADNSACPWADTVLYAVSLYYRLRFGNGLARPRDWDELGRVVAELDRTFSRKRTVRRAK
                     TAHSDGLK"
     gene            complement(31241..31600)
                     /locus_tag="PLD05_02810"
     CDS             complement(31241..31600)
                     /locus_tag="PLD05_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOP76390.1"
                     /translation="MSADDAMGQDVGEIQVRPRRKRRERTDTRHIPRYHVILWNDDDH
                     TYEYVIKMLMQLFGHPFEKAYQMAKEVDLSGRCIVLTTTKEHAELKRDQIHAFGRDPL
                     VAECRGSMTATIEPAAE"
     gene            31751..33148
                     /locus_tag="PLD05_02815"
     CDS             31751..33148
                     /locus_tag="PLD05_02815"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOP76391.1"
                     /translation="MDNAIARVKDFLEAHRTTAEQFLSEFLAIPSVSADPQHRPDMKR
                     AAEWVAQFFSQMGLAAELIQSPTGHPVVYAETPHCEGIPTVLIYGHYDVQPAEDPTLW
                     QTPPFEPTIRGGAIYARGASDDKGQVLTHLLSVGAWYQVTKSWPLNFKFVIEGEEEVG
                     SSVLEKFLDEQADRLACDCIVISDGNQFGPDQPAITYGLRGIAYFELRVKGPHRDLHS
                     GSFGGTVTNPATALTRMLGQFMTQDGRVAIPGFYDDVLPLSNEERRRLRELPFDEPAF
                     WASLGVETAWGEPGYTVLERRWARPTFEVCGLWGGYQGDGAKTVIPANAGAKISFRLV
                     PHQDPDRIREALLSYLAQICPPGVQYEVIDLHASPAVLTSLDSPYIKAAENAVKMAFS
                     RSPVFTREGGSIPIVSRFQQRLGADVLLLGWGQDDDNTHAPNEKFSLADFHRGTLASA
                     YLWEFIRQIGKATDS"
     gene            33278..34555
                     /gene="serS"
                     /locus_tag="PLD05_02820"
     CDS             33278..34555
                     /gene="serS"
                     /locus_tag="PLD05_02820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOP76392.1"
                     /translation="MLDRRFILENTDAVKRNCEIRGVVADVDRFVELERQRKELQSQL
                     DAINRQANEVAKSIGRCTTAEDRESKKEEGRRLREQAAQIEKQLQQIAAESDSILKQI
                     PNMTHPAAPIGDETASREVGRGKTPIRQFDFVPKDHVQIAEELDLIDFEAGARVTGHG
                     FYFLKNEAVLLELALQKFAIDLLRSEGFVVTTTPDLARNDILEGIGFLPRGPETQIYS
                     IENTDLSLIATAEITLGGYLADQVLEAEQLPIKMAGISHCFRTEAGAHGRATRGIYRV
                     HQFTKVEMFAFTLPDQSDDMLEYFRGLECSIFDQLGIPYRVIDTASGDLGGPAYRKYD
                     LEAWMPGRGEFGEVTSTSNCTDYQARRLGIRYRVKGEKGTRFVHTLNGTAVAISRALI
                     AILENYQQKDGSVVVPEVLRPFVGLERIGPRRS"
     gene            35700..>36574
                     /locus_tag="PLD05_02825"
     CDS             35700..>36574
                     /locus_tag="PLD05_02825"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOP76393.1"
                     /translation="MNEANPKAQTRRIVIFDTTLRDGEQSPGCSMNLQEKLEVAHALA
                     QLGVDVIEAGFPIASPGDFEAVKAIAESVRGPVICGLARCRPEDIDRAWEALRFAESP
                     RIHVFLATSAIHREHKLRMTKEQIIDVAVAGVRRAVGYCPNVEFSPEDAVRTEIDFLC
                     QVVEAAIDAGATTINIPDTVGYATPGQMFRLITTLRERVPNIGKAVISVHCHNDLGMA
                     VANSLAAVEAGAGQVECTINGIGERAGNCALEEVVMALRVRQDIYHADTGIVSQRLVP
                     TSRLVAGITGMVVQR"
BASE COUNT         8307 a        10150 c         9848 g         8069 t
ORIGIN      
        1 gcggcacgcc accgttgcct tccctctccg tgctgtgagt ttcaagatgc ttaagagact
       61 atcccaaaaa gtccccctct cccttcggga gagggttggg gtgagggcaa gcgaatgtcg
      121 gaatcgattg agaaaaccgg catcgattcg agtcaccctc accctcgccc ccgacccctt
      181 tcccgtcgaa cgggcgaggg gagattatgg gataggcttt aagcgggcac cgtcgatcag
      241 gcgtaatcaa gtttcttcgg tggattcgaa ccaatcctcc gcccacacat cgtcatcaga
      301 gggttcctca tcgcgttcat cgagggctgg gattttggat gcgacccgcc gactctctcg
      361 tttccgcaac aacaaacgga tgggaacttc cggataggga agttcttccc tcatcgcgtt
      421 caacagatat cgttgataac tggatgaaat gagcgacggt tcattacaca cgaggacaat
      481 cgtcggcggt tgaaccgcca cctgggtccc atagtagatt ttgacttgct tggttcccac
      541 caaaggtggt ggattgctct tgagaagacg ctggagtacc ctgttgagac gtccggtgct
      601 cacccgctgc cgagactgtt taaacaacga ctgggcaagc tcgataagtc gttttacgtt
      661 ctttcccgtt ttgcccgtta taaaagccac gggcacataa ctcatcgttc cgaaggcctt
      721 ggccagacgg tccacccata catccgttgg tatttgccct accatgaggt cccacttgtt
      781 gaccacgaaa atgcagggtt tgtactgttc ggcaatataa gcggccagtt gtttatcgac
      841 tccggatata tcctgtgtgg catcgaagaa aagcaaaacg acatcggcgc gtcgaacact
      901 tgcctcggcg cggcagacgc cgtaaaagtc aacgttttcg cgcacactct tgcgccgcct
      961 gaggcccggc gtatcaatag cgacaaagac ccgcccatcc atttcaaagc ggacatccac
     1021 actgtcgcga gttgtgccgg gtatttcgct gacaatcatt cgttcttctc gtgccagtgt
     1081 gttgataaac gtacttttgc cgacatttcg ccttccgacg acggcgattt tcatctccgg
     1141 ttcgatggcc gccttatcat cctcgctgag ttcctgaggc agaagatcga gcatggccgt
     1201 tatcaattcg ctcttgccgc gtctctgttc ggcacttact ggaacgatcg gccaacccag
     1261 cgaagaaaac tcttgcgctg caggttccag gcttggggca tcggtcttgt tcgggacaca
     1321 aatgacgggc cgctgaaggg tgtgcagccg gcgggaaatc tcctcgtcca gcgccgttaa
     1381 tcccgctcgg gtatcgacaa caaaaagaat caggtctgcc tcgtgtaagg ctatctcaat
     1441 ctgtcgggtc acatggtcct caagctgatc gacatccacc aggcctatgc ctcccgtgtc
     1501 gatcaattcc accaatcgct catccagttt ggccaaggcc gtaactcgat cgcgcgtaac
     1561 acctgcccgc gcatccacca cggacacacg gcgtccgacg agccaattaa ggaggctcga
     1621 ctttcccaca ttgggacggc caacgattgc cactttggga atagccatct cttcaccgaa
     1681 acccgatttc gcaaaaaccc cgttgggcac aaatccggcc accgggcaca tttccaatct
     1741 tctaaggctt atcccgaaat cttccctcgc ccgttcggcg ggagaggggc cgggggtgaa
     1801 ggtggctcaa atcgttgctg gttttctcga tcgactccga cgatctcttg ccctcacccc
     1861 aaccctctcc cggagggaga gggcgacttt ttgggatagg ctttaaatca acttgagtct
     1921 gtcacaactg gccaaaactt tccgcccgtt ctgtcctcag ccattgattg taccggagag
     1981 ttcacaataa gtttattcac ggcaaattgg ttttactgca tgggaatgac ctgagaacgt
     2041 ttttcattgt cgaggtggac catcgcgtaa cgaagagtag ccttgtggtt ctcacccgag
     2101 gctgatcagc ggcccatccg cggaacacct ggggcagcgc aatctgccac acgactttat
     2161 cgtacggatg caacttggaa cgccgggcga cgttgctgcg ccacttcgat ggccttgaga
     2221 agccctctcg ccttgttcat ggtttcctgc cattcgcggt ggggatcact gtcggctacg
     2281 ataccggccc cggcttgcac ataggccttt cccccttgca cgacgattgt ccgcaacgtg
     2341 atacacgtat ccatattgcc actgaaatcg aagtaaccca ccgcgccggc ataaggacca
     2401 cgccgatgcg gttccaactc gtcaatgatc tccatggccc gaaccttggg cgccccggat
     2461 acagtgccag cgggcaaaca agcctgaagg gcgtcaaagg ccgtcttgcc gggcgcaagc
     2521 tcccccgtca cgttggaggt aatgtgcatc acatgactat accgctcaac agtcatgaca
     2581 tcggtcagtc gaatcgagcc atagcggcaa acccggccaa ggtcgttgcg gcccaaatcc
     2641 acaagcatca cgtgctcggc ccgttctttt gggtccgcca aaagctcttc ggccaacctt
     2701 cggtcctctt cctcagtctt tccgcgaggt cgtgtccccg ccaagggccg cgtggtcacc
     2761 tgccgattga caactcggac aagaatctcc ggtgagctcc ccactaatgt gacgctggga
     2821 cttcgcacat agaacatgaa gggactcgga ttgatgacac ggagggcccg atacacctca
     2881 aacggatggg cctgaagggg tacctccaga cgctggctaa tgacgacctg gaagatatcg
     2941 cccgcttcaa tataacgaat gcactttcgc actgcctctt cgaagtcttc ccgcgtgaag
     3001 tttgatgtaa actccacaca gggttccgtt tctacgcaaa tatcaaaagg ctccagttca
     3061 caagggctat gcgtcagttg atccaccgcc tcatcaatcc gccgacaggc atcggcgtag
     3121 gcatcttccg cagtccatcc tggtcgatcg agatgcgcca acgccaccac gatgagagac
     3181 tttgtcacgt ggtcgaacac gaccatccgg tcataaaaac caaacgacag gtccgggata
     3241 ttgcggtcat ccggcggcgg attgggtaac cgttcgtagt aacggacgac atcgtatccc
     3301 acgtagccaa cggcaccccc acaaaatggg ggaagctcgg gcaacgtccg gacacgtaag
     3361 ccttccaggc gtttccgaag ctcacccaag gggttatccg aaatgaactg gcgcgtttcc
     3421 aagagcggtg ccccagacgc cgaaacgccg gtgtatgagt tcacaatgac tcgccgctcc
     3481 caggcctcga tttccaaaaa gggatgcgtc gccaagaaac tataacggcc aaccttttcg
     3541 ccgcctgtta cgctttctgc aagacatgca cacctgcccg catcgagttt gtggaatgct
     3601 gttacgggag tcagcgagtc gctaaccaaa cgccggtata ccggcacaag gtcaaatccc
     3661 tcggccagtt gctgaaacga ttcaaaatct ggatagtatc gtttacgcga cataaaagag
     3721 atctgtgatt ttttgaaatg gggcccccaa cgtctctcat taagtatcgt ctcacaatgc
     3781 gtgggataca atcatagcgt atcagtggga agaaagcgtc acaatagaac ctcagaagct
     3841 cgttctgacc tggcggccac caaatcattt gtgacatgtt gctacagcgc cttgtgacaa
     3901 cacgtgtcac ttgcggcgtc ggaaaagccg cacggcgcgg tccagacctt cgatcaatcg
     3961 gtcaatctcg ctcgccatat tgtagaagta aaagctcgct cgaacagagg ccgtaacacc
     4021 caggcgtttg tgcaacggca tggcacagtg atggccggca cgcaccgcca cgcccacact
     4081 gtcaaggacc tgtgcaacat catgggcatg gatgcgatcc acgacgaagc tgacgattcc
     4141 agacttgtac tccggcgttg gccccagaat acgcacgaat tccagtttct ccaattcctc
     4201 atgaagcctc tttgttagtt ggcgctcata ctgatcaatt cgctccaacc ccacttgctc
     4261 gagataatca attgcagccg tcatcccaat ggccggcaca atcggaggcg ttcccgcctc
     4321 aaaccgatag ggaacgtcgc ctggggtaaa gccatccatg cgaacttccc gaatcatgct
     4381 acccccaccc ataaatgggg gcatcgattc gagaatctcc cgtttgccgt acagcacacc
     4441 gaccccggag ggtcctagca tcttgtgccc gctgaagacc aaaaaatctg catcgagcgc
     4501 ctggacatcc gttttgcgat ggggcacgcc ttgggcaccg tcaacaagga ccaaggcccc
     4561 taccgagtgg gccgccttaa taattacggg aagtggaggg actgtcccga ggacattcga
     4621 cgcagcagtg acggccacaa gcttggtccg agaagacaaa agtcgctcaa gttcctccat
     4681 caccaggagg ccgtcgtcgg tgatcgggat ataccgaatc acggcaccgg tacgttccgc
     4741 tagttgaaac catggcacaa ggttggaatg gtgttcgagt tccgtaacca atatctcgtc
     4801 accttcgtgg acgttggcgt ctccccacgc tcgggcgacc agattgatcc ccgccgtcgc
     4861 tccagaggta aagatgacct ccgtctccga actggcgttc agaaaccggg cgattcgttc
     4921 ccgggcaccc tcgtaaagct ccgtcgtttc gtcggccaac cgatggatgc cgcgatggac
     4981 gttcccgtag tgtgtctcat aaagcgttgt aatagcctcg ataacctgcc gcgggcgctg
     5041 cgttgtagct gcgttgtcga agtacacgaa tggacgtcca tcctcaatcg tcctgttcaa
     5101 aatcggaaag tcagggcgat atagttgcgg atcaaggatc gcggtttcat gccgagtcgt
     5161 catgagcgcc tctcagtaag agtccttagg ttggctattc ttctgccgga agcgggatgg
     5221 ctgccccacc tcacgctagc catccactcc gcgattgcag cgatttttca attgtagcaa
     5281 accccgtcaa attaaggtgg gcgatcttct cagtccggag aaatcggccg caaaaactca
     5341 agaagagccc aatagttcgc cctctggtca tggacgctgt gaacgtcgct ccagcaccga
     5401 tgacgccgac gcgaaggtag ctgtggaatg acgggtttca ctaagagcct atcccaaggt
     5461 tccctctccc tctgggagag cattggggga acggcaagtg atcgtcggag tcgatcgaga
     5521 aaaccagcag cgattcgagc caccctcacc cccggcccct ctcccgccga acgggcgagg
     5581 ggaggcggtg agataggctg taagccccca ccgagccgaa tacgcgaacc gactgggcga
     5641 gaagccacaa agccgccggg tttttcgaga ctcacttggc aacgctttct tttggggcct
     5701 ctggaattgg gggcacttga ggacagggtg agtaaatggc ggcttgaagg acacgccagg
     5761 gcaacagggc acacttttga cggttgggcg tcaaccttgg cccaaagtac ttgagcatgt
     5821 cctgtgcggt gaacttcttc acatcctcga ccgttttgcc atcgaacagt tccacaagca
     5881 tcgaggcagc agcttggcta atacagcagc cttccccgtc gaaatagatt tcccgaatct
     5941 tacccccatc gataatcctc agctccattc ggacgacgtc accgcacagg gggtttttat
     6001 cctcatggca gtgggtacac tccgagcacc tccccttatg gtagggggat tcgtaatgct
     6061 ccaggatacg ttcttggtag atttcgtcct gatccattgt tttcacaact tccgcctaca
     6121 agcgaaaaac aagcgtcaac gacattctac tcttgggggg taagataggc aagttgcctt
     6181 atttaccgca acaagggcga tacaatactg tatgcaccgc tgatggtcgg aacaacccgt
     6241 gaccgacatc ccgcgacgtg ccggtcccta tatacgttga atcacaatgc catgttccaa
     6301 ctggtaagcc catttgaacc ggccggcgat cagcccaagg ctatcaaggc cctctgtgag
     6361 ggccttgata aagggaagcg atttcaagtt ttgatgggcg ttacgggctc gggcaagacc
     6421 ttcacgatgg ccaacgtgat ccagcacgcg caacggccta ccttggtcat gtctcacaac
     6481 aagacgttgg ccgcccagct ttacgccgag ttcagagaat tctttcctta caacgcggtc
     6541 cactattttg tcagctatta cgactactat caacccgagg cctatatacc ccagcgcgac
     6601 atttacattg aaaaagacgc ctcgatcaac gagcaaatcg accgcttgcg tcttgcggcg
     6661 accagcgccc tcatgacacg tcgcgacgtg atcatcgtgg caagcgtttc atgcatctac
     6721 ggcttggggt cgcccgaaga ctatcggacc atgacggtgc ccgtcatcaa gggtcaggtt
     6781 cttgaccggg acgagtttct cttgaaactt gtcaacattc agtacgagcg aaacgatgtg
     6841 gagttccagc cggggagatt tcgcgcacgg ggggattgtg tcgatgtttt tcccagctac
     6901 gagccattcg cctaccgaat tgagttctgg ggtgacgaag tcgatcagat tgcctccatt
     6961 catccggtaa ccggcgaagt gttacaacgg cacgaccagg ttttcatcta cccggcaaag
     7021 cacttcgtct taccagagtc acgtatccag gcagctattg tgagcattcg cgaggaactt
     7081 gagcagcgcg tggcggagct gaaatctcag ggaaagatcc tggaggccca ccggctcagt
     7141 gcgcgaacac gatatgactt ggaaatgctc caggaagtgg ggtattgtcc cgggatcgaa
     7201 aactacagtc gtcatctcgc aggacggcct ccgggaagtc caccggatac cttgttcagc
     7261 ttctttccgg acgatttcct cctgtttgtc gatgagtcgc acgtcacaat accccagatt
     7321 cgggggatgt ttggcgggga cttcagtcgc aagcaaaccc tggtggagca tggtttccgt
     7381 ctgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngattt gcgagagggg
     7501 gcctttgatg ttttggtggg ggttaacctc ttgcgggaag gcttggacct accggaggtc
     7561 tctctggtgg caatccttga tgccgataag gaagggttct tgcggagtga gacctcattg
     7621 attcagacca ttggccgttg cgctcgaaat gttaatgcca aggtcatcct ctacgctgac
     7681 aaaatgacgg attccatgca gaaagcggtg gaagaaacga ggcgacggcg aaaattgcag
     7741 gaggaatata acaaggcgca cgggatcact cctgaaacga tcaagaagaa catccgcgct
     7801 ggcatcgagg ctgaggttgc ggcccacaac caagccaacg ccctcgtagg gcggtctgac
     7861 gaggctcagt acatcaccga agagtatatc gccgagctcg aggccgaaat gctcgccgcc
     7921 gctgaggccc tcgaatttga gcgggccgcg gctatccgcg accgaatcca gcttttgcga
     7981 aagcagatcg gcaagcccgt cacagaggcg cagttgaaat cgccacaaaa gcgccgtcgc
     8041 ggtggacgca gaaacaccat ccacccttcc cccggaattg atatcacaca gttccggcga
     8101 aaatagaaca aacccgggct atccaaagag cggggtcaac aacgttgccc tttgcaataa
     8161 atggaggccc ttcgacgggg attagccccg ccgaagggcc ttccggacat agcgctcgat
     8221 acgtcggcct aactgacggc cagtttcaaa atgataatca ctttgaagcc aagaggttac
     8281 tgctggcttt caccgccggt gccttgctca gccggtgcct cctgggattg ctcggccggc
     8341 tgggcggatt catccgatgc gggagccgac tcagaagatg cggctggagc tggggcacca
     8401 ccggccggtg ccggcgcctg cttggggcag ccaacgaaca atgaactcaa agccaagacg
     8461 atcaacaagg atcccaactt cttcatccga gatacccttt ctcccaaaaa accgttaaag
     8521 gcacccccga ccgccggagg tcgcgtacaa aaaagtacga tgcgctccgt tggtgtttta
     8581 ttcccgcgaa cgttacattt aatggaagat acccggctgc gaatatgcca aaggtaagca
     8641 tctggggaat aatggccggt ggttgcgcat cttaatgggt agtcaaagat ggatgaaggc
     8701 tcgcggcagc ccacgattga ggaattggtg gtccagcacg ctcagacggt ttttcgctat
     8761 gcgtaccgtc tgtcggggtc cgttgccgat gcagaagatt tgacccagga gacattttta
     8821 ctcgcagcca agaatattgc ccagcttgag catccggcgc gtgcccaggg gtggttgctg
     8881 gccatcacac gaaatgcctt cctcaggcag cgaagagggc cccatgttac gcttttggat
     8941 gacatggcgg caaccatcgt tgaatcgcaa ctggattcgg atgataccga cgaacaattg
     9001 atccaaatcg ccctccaatc gctgcccgaa cagcagcgcg tcatgatcct catgttttac
     9061 ttcgaagata tgtcgtataa ggacatcgcg cgagaattgg gcgtcccaat cggtacggtc
     9121 atgagtcggc tggcgcgggc aaaggcagct ctgcgaaaga agctcgatgc gctcaccaaa
     9181 aaggtggccc ctgtttgaaa tcccaggatt taaaaaatga tgactaacaa taatggcctc
     9241 ccaacccatt gaccctaaag acgaggacct cctagagatc atccaattgg atcgagatga
     9301 tccgcaggat gcgttcgcgc agcggctctt ggccatcttg cgggaaaatc ccgccctcgc
     9361 caagcgctac gaggaaatcc gccgacgcga tgccgagata ggaaatcggc tcaaacaggg
     9421 gaacaagctg tcaggattag aagaacgaat tttagcccgg ttgggatatc agaacgaagc
     9481 gaccgataat cacctaggat cgtctcagac gctcgatgtg gcacaccctg agtctggggc
     9541 gttgcgttcg agtctgtgcg aggggggagc ttctccagta gtcacgtctc gtgtagtcac
     9601 gggagcacca gtgcctcgcg ttggccgtcg tcaatggtta cgttgggtgg gggctttggc
     9661 aaccgcgggc gcagtgggag gactgggaat ttgggtctgg cagcgccgaa aagaaaaacc
     9721 tcgtatctcg gcactcgccc ttggaaatgc agccaccgaa ttgttcgaag ccacattgga
     9781 cggttttggc accggtcgct tgcttggacg ggaaaacccg ccccagggtt accgattgag
     9841 tcgcgacgtg caggtatttc gcggaacggt gattacctgg cggcaagtca cactcgcgga
     9901 tttgcccgcc atcgtttttg atctcagtca tccggggggc gaaaaaggtt cgctgtttgt
     9961 gatccggctg gtccttcctg atcttccgcc catgccaccg cgacggcctt gtttgcgaac
    10021 aagtcgctct tcagccggtt ggtggcaaga caatgatctg gcttgcgttc tggttgtact
    10081 gggaggaccc cgcgtgtacg agcggttcct tgccccaccc gcacccttaa cctaaggatc
    10141 caggccttcg cgcggtctgg gcccgggttg ccagcctctt aagacaatga agttgggcat
    10201 cagatacgtt tgcaagcgtc cctcgggcac aaaaccgcga ggtcaggatt ctacggcttg
    10261 ccacccaaag ggtatgtact ggtaggaaat ctgattggcc ggactgatgt cgataatcgc
    10321 gtagccctcg ggaaagccct tgtgaacgcc tttccaccac cggcccgaga cggctcccga
    10381 acaagcgaat gtgatccctc gatacgatac ctcatccacc atatgcatat gacccgataa
    10441 tgctagcttg acgttgttgt cggcaagcaa ttgggccatt tgctgggcac ctgcacacat
    10501 ggacgcagaa ccgatctgaa atcgtccttc tttagcgctg tcggggagca ccgtcaccgt
    10561 gataaacggg atatgggtca cgatggcagc cggcatttcc ttgggtttgc gggccagctc
    10621 ctggcgaaac caatccattt gaaggccgtc gattcctccc tgatacaatt ccgtttccga
    10681 aggttggaca ctatccagga cgaagaaccg ccaacccttg tggtcaaagg cgtagtaagg
    10741 gcgttccagc tccatctttt ccatgtacat cttcttgccg tatttgggat gctcctcggt
    10801 aacaccctgc tttttccccc agccaaagac atcatgattg cccatgcaat ggtacacagg
    10861 gaggctattg tgatcgcgga agactttctt cagcagatca aacagcatcg acgcgcgttc
    10921 ctccccttgt tgaagtgcat ccatgatcaa atcgccaccc gtgaggatga agtcaggttt
    10981 tggatttagg gattgaatat gatgcaggca ggcagcgacc ccttgcgcgg cctttaactc
    11041 cggctgaatg tgaaggtctg tcatgtggac aaagcgaaag ccatcgactg aaagggagga
    11101 attagaggaa cgctcttccg cccaagtggc aacgcctgat cccgataacc aaccaccaac
    11161 cagcgcgccg acgcccgcaa accaaccgcg acgtgtcaat ttcatcatcg gtctcctctc
    11221 gatattggaa aagtgggaaa ggacaacaca ggtttatcga gattctatcc gatgaaaggc
    11281 cgatgaaaag tcttcgccgg tcgcgttaac cgttttttaa caagacagat cggcagccca
    11341 atatggtcgc ttaagccaac aattggacgc gggcaaccac ctcgtccaag gtccacggtg
    11401 tttgacggtc caacggcagc ataacatagg ccgccttgtc agaggagcag tccaaaatga
    11461 gctggacatc gtttggagtc gcaaaacctt gtggacatgg ttcatgaccg tgaaccagta
    11521 ccgccgcttg ggtaaggcgg gcaaaggcca ttgcgttttc gcgacggtaa tcgcgtcccc
    11581 aaaccaggcg gaaaacgctc ttgtgctcga gaaactcgac catgtcgagc ggacgatgaa
    11641 atactgtgac gtcaaagcct tgtcgatcga ccgcttcggg aagactgtgg gttaccagaa
    11701 gatcaccagg taagcggacc ccaagagggc aggacttgat gaaggccaaa tacgattcgc
    11761 ggacgcggtc ggcagccggc ccgtaaacat gttgcagccc cagccggaag agcagattca
    11821 acatctgctt gttcttttga attggataat ccgtcacctc tgacaattca tggttaccca
    11881 gaatgaaatg gacgcggtca gggtactgta ccttcaattt ggccacgtct tcgagcacgg
    11941 tatgagacat acaccccgca ttggcgggat acgtcggccc gccgtgacag acttcctgca
    12001 agatgaggtg ccttttcggg ttcttgtcca aagccgccac tcgccggatc aggttaaaat
    12061 tccggcgatt cccatggatg tcccctgtaa ttattacatc ggaaccgtct tccggggtca
    12121 aaacaatgag atttcctcga cgggcgggcg tctcttcgtt cgcccgggcg gcattccggc
    12181 acgttgcgac gatttgctca atgtattcga atgtggcaat catcctgata agcctcaagg
    12241 ctgatcatag caccaaagaa ttggccacct tatcgtcata tctgcgcgat atccgttatt
    12301 atcttggtca cgccggtgcg aagaaaggcc catgtgcgca cccaaaaaag ggccccatct
    12361 tcacagcaca acatgaaaga agactcttta tagcagcagc gacgggggta tgagaaaaag
    12421 tagcgtcccc tcgcccgcgg caattggtcc tttgaattgc accgcggcaa ttccagcctt
    12481 tgcgaaatgg aaagtgcccc cgccaatgag tgtggctaga atcttggaaa cgtctggggc
    12541 atgtccaacc catgcgatgt cgccggaaag ctgacccatc ctcgtacgtg tccattcaat
    12601 caattggtga atatcaccct gcggtgcaag gaacggtacc gtttccagaa ccataggttc
    12661 ttgccaactg ctcaccaaga tttccgccgt ctccacacac cgcaggaaag gactggtgac
    12721 tacgacgcgc ggggcaaccc caaaccgacc aagccgccgt gccacccgcc gaaacttcgc
    12781 ccgaccttct ttcgttaagg ggcggagact atcatccggc cagcgggcct cgttaggttc
    12841 ttccgcccag gcatgtcgta tgatgtacac attcatgctt tcaacccatt aagtgcgttt
    12901 tttgagatac gggctcgcga aaacctcaag aaatgtcctt agagaatatg gccctttacg
    12961 atcgcaacga tcctccacac taaccgtagg cccccaaacc gaaaaagagg tgttttttca
    13021 accaacgata ggtacccatt gtctcgggaa gttcttcgcc gggaatgtga tgacttgccc
    13081 gaggagagga tgcccgcagt tcggcaaaag catcccggcc acttgtatca cgggcatggt
    13141 gttcacgcaa gaacgcctcc agttcatgag ggcattcgag gatgggacat ccgcgagtcc
    13201 gagaaagtgc gaatttctga aaaccgcgga ggaaatcact cgtgttaatc gtatggaaaa
    13261 catcaccgcc gtgatcacga atggtttctc gcgcaaagga aaggggcgga cacagttcaa
    13321 tatcgccggc tggtcctatg tggtagccta atcccaatgc tgcagggcac actgctttgc
    13381 catcggcgtc ccaatatgta tcgacaatga ggatcggcga aatcctccgt aatcttagca
    13441 actgtcgtcg aaccttcagg atgtcggctc ggcttaaggc cagttgtggc attgggtcgg
    13501 ggccgacggg acgatatatg tagtaccaaa gatagattgc accctgctca tcgacccaat
    13561 taacatactc gggacttagg gccgcttcat agttctccga tgtgatcgtg gtagccacac
    13621 caaagagtat tttgcctttt ctcaggcgtt ggaaggcttc cttaatagct tgaaaagtcc
    13681 cttggccgcg acgcgcgtcg ttagcacttt cgaaaccgtc gaggcttacc aaaatcgtgg
    13741 cgtttcccac ttcctttgcc gtagccacag ccgcttcatc caagaggagc gcgttggtga
    13801 tgatttgaaa atagcaatcc cgattctgcc gtaggattgt ccaaaggtcc ttataaagaa
    13861 acggctcacc acccaataac acgaaaaaat ggttgccgtg aaccttggct tgggcaatga
    13921 agtgattgac gtcatcacga ctcagttggc gcggttgccc ctggcttgtg atccaacaac
    13981 cgtgacagcg aagattacag gcgtcggtga gggaaagaaa gaaaaacggg ggaaaaagct
    14041 ctgaggcggc cactcgtttc cgaaaccgcg acaaagaccg caatcccggg ataagccaca
    14101 aacgaccggc cttcaggagc aacctggggg aaacacgccg gagtaatcgc caagaaaaac
    14161 gactcaaaaa acccatctcc ctcgcaactc catcagttgg tcgatacagt cggcaatcgc
    14221 ttgtgacaaa agcctgccat aaaccgctat tttaacgtca cgattcttcg cagtcgcccg
    14281 tgtgttcaag tggcctacga tacctgcggg tccgcgctac ttccaacctc gactttcaca
    14341 acgtcccgac gataactccg gcaatcaatg gtacatcgcc ccgaattcgt aaacagcatc
    14401 gaacatggcc acaatatttt cgggcggcac atttgccaga atgttgtgaa cctgttgaaa
    14461 gacaaacccg ccaccgggtg caaaactctc aatctgttcc cgaacgtgct gtttcacttc
    14521 ctccggggtc ccaaaactca agacccggtg ggtgtcacaa cctccacccc agaaggtgat
    14581 ccgctttcca aaatcacgct tcaattctgt cgggttcatt cctttgcagg aaatttgaac
    14641 gggattgatc gcgtctaacc cggcatcaat caaatcatcc agcaactcac ggacaccacc
    14701 gcaacaatgg agcatcactt tgacgtccgc taactccttg gcttttttcc acatcgcggc
    14761 atggcgcggt ttgaagagct ctcgatacat cttgggcgat atctggggtc cggactgcat
    14821 ccccaagtca tccccgaaaa cgatgatatc aatcaatccg tccaaaaccg ggagaacgcg
    14881 ctcgagattg gctagatggg cttccacgag cgaatccaga accttttctg cccgcggcct
    14941 gtcaccggct aaaagcatgt aaaagttatc attccgataa agaaattgtc cgcactccag
    15001 caggtttccc ccaaacagaa acaggatggc acgatcagtt tgttggcgta ggcgtcgggc
    15061 gccttcccgg aggcgatcca atccgcccgg accttccaca ataggtcccg gaggcgaggg
    15121 tattccacac caaatcgtat cgatcaaagc ggaagccgcg cgctctggct ggtctttttc
    15181 cgcgaagggc cagtaacatt gctcgaaata cagggcacca tcgggcatcc gccctaggat
    15241 tcttcccgtc ggtccccgca ggacccaccc gccgtcactg cgttcgggca ccgcccaact
    15301 gggcatttga cacggtgtac catccggcaa cacccaatcg acccaataac gatcctcctg
    15361 ggcaaaggcc cgacctagct caatcgtatc gactcgaaag cgattgagga catcctcgtc
    15421 aatgatcgcc aactgctgaa ttggatcgta aacgcgaatg ggacgctcgg gcagccccag
    15481 atatttcctt aaacgggcgt aagcaatagc cgctatacca gaggagcgat gaccaccaaa
    15541 gtcaatcggg atccgatcag gctcatgatg ttccaatgcg gccaagactc gctgctttgg
    15601 tgtcattcga atggcctccc gcactgctag cgcatcccgt aagaacaaac cgcgtacgag
    15661 caggtgcaaa agttacccga agcgatgtga ttgatatttt agtatgtgaa aatagggaga
    15721 aaaagagccc ggtaaagacg agcaacttcg ccacgttggg ctcaatctca atcgcgtcac
    15781 aaggttaatc tcagtggcgc cacaaggtag gaaaacgctt cacaaggcgg gacaatacca
    15841 tcccatctga ccaggcgacc gatgcagcga gatgcccttc taccggtatg ccacagtcac
    15901 gcggcgctac ggaccgcttg ttccgaagga atccgcaact tcggtgactt cggctgggtc
    15961 ttccagcgac gatgaatcca ccagtactgt tctggggcag agcggatcat ctcctccagt
    16021 cgggatgtgt accactgcgt cacaaacgtg acagcgtcgg gggctgagac atctctggga
    16081 tcgagaatgc ccaccagccc catctcgaaa tgcaaaggct taccggttct ccgcgcgtaa
    16141 accacggcaa ttggggcgtc atattccagc gaaagcaggg caatggcctt gaaggtcgag
    16201 gccggtcgcc caaagaaggg aacaaaacag ccttttcttc ccgcatcttg atcggcaagc
    16261 accgcgatgg tgtgaccctt ttgaatcatt tcttcgacct ggtcggcccc accaaccttg
    16321 ggaataaggt attgtccccg tgcagcgcga aatcgttgca caaaacgatc gagaaaaggg
    16381 ttatccaggt ttctcgcgat tgttgcgctc ggaaacccca aaaggccaag catgtagccc
    16441 cccagttcaa agttgccaaa atgtgccgtc acaataatga caggtcgatc ctccaaaaga
    16501 agtttcacca gcggctccac attcttgagc gttgcccttt gacgccagtt ctcttcccgg
    16561 attttcgctt gtgtgtgggc tacctccatg accaaaagaa aaagatgatg ccacattcgt
    16621 aaaatcaaat cgcgacgttg agaaggagaa agctccgcaa aagcgtaccg gagattctcg
    16681 tcggccacgg aataacgaat ccgcaaaacg tatgtgaaaa ggtatgctaa gcaccacgca
    16741 agccaagacc ccacggcaag tggaatcatt tggacgacaa agatcaggaa tcggacaatc
    16801 acgtaaacaa taaagtgccc aaggagtgcg actcggtgca ttgatccctc cgtggcttgt
    16861 gagctgcgca gcacgttcaa cgcgatctac cccaagaatc tggccgcggc tattccaggg
    16921 cgaattgctg aaatgcgagt tgcaacttgc ataaacttag cggaaaacgg gcgacttcgg
    16981 caagatgtcc tggtagcagg ttaaatctgc tcggagcgcc accccgccgt gtcctgttgc
    17041 acagtaaagc tcgaatatta cactggtgac atcaggcaaa ggcaagcgct ttggtcacag
    17101 cggttttgtc ttgggagcgc aaatcatgtt cgcacgttgg ggaggtgttt tacttgctgt
    17161 ttggtcgctg ggtatcgttt tcgtgggatg gccccaagga ttggcgtgtg aaccagggcc
    17221 ggagttgcga acatcgacgc cctcggtcga aagaccaaac cttttggtca ttatcaccga
    17281 tcagcaacac gcggggatgc tgagctgtgc aggaaatccc tatcttcgca cgccggcgat
    17341 ggattctttg gccgctcaag gtgcgagatt tgagctggcg tacaccgcca acccggtatg
    17401 catacccgcg cggatgagta tggtaaccgg acgaatgccc agtgcgtttc aaattcggtc
    17461 caacgccgaa ggaaagaacc ccgcccccaa ggaggcaatc gatcaggcaa tgggctggat
    17521 cttccgccga gcgggctata agaccttcta cggcggtaag acgcactgga tgcgagggat
    17581 gacgccccaa agcacggggt ttggggaact cacgaaggat acacgggagg agttggccag
    17641 gaagtgcgcc gagtttcttt cggagccacc ggctgagccc tttctgcttg tagcatcctt
    17701 catcaacccc catgacattt gtctcatggc aattgacgcc ttcacacagg caaccggacg
    17761 acctgctata tatccggaac ttcagcgaga ccgcgagtgc ctggcagagg ccttggccct
    17821 tcctgccggc atttctgaag ccgagttctt cgaccgactg tgtcccccgt tgcccaacaa
    17881 ttttgagatt ccatccaacg aacccgacgg cgtcacccta agttacctgc ccccacaaag
    17941 ttttcgggcg tatgtccgac agcattggac agaacgggat tggcgtctcc atcgctgggc
    18001 ctattgccgg ttgaccgaac gcgtggatgc cgaaatcggg attgttctag atgccctccg
    18061 gcaacacgga ctggatcgaa aaacggtggt catctttaca agtgaccatg gcgacctgga
    18121 cgccgcccac cgtttggagc ataagtcgat cccttacgaa gaggctgtga gggtgccttt
    18181 tattgtcgca tggccggggg taacgaagcc gggcctggtc gatcggacac acctcgtttc
    18241 ggttggactg gacttgattc ccacactctg tgactttgcg ggaatcgaga agcctcctca
    18301 tttgctgggc ctcagcgttc gacctttggc ggagggtcgc accgtggata cgtggcgaga
    18361 atcggtcgtg gtagaaagtg acgccgctcg gatgatcagg accgcccggt acaagtatgt
    18421 ggtttatgaa aaaggcacga gacgcgagca acttatcgat ttgaagaacg atccgggaga
    18481 aatggaaaac ctggcgggtc agtccgcata tgatcagatt ctcaaggagc accggcaact
    18541 cctgtgggag tgggtgacac ggattggaga tacaattggt caaagctttg tcattgctcc
    18601 tgatgagcgt taaaccagaa tttaggcgtt aatgaccgaa aaacatcgcc aacttgcgcg
    18661 gaaagaactc gagaaacacg tccagttgcc aggaatcact gcgaggagct ttaccccatg
    18721 agtagattgg gacatacccc agactggtca cgacggaaat ttctcacggc gagtacggcc
    18781 ggacttgcag gtttggccat cccggtcagg agtgcctcgg cgaggtacga tcctcctgtt
    18841 cacccgcaat tccaaaggct ttcccaaaac cttggccttt taagggggac cattcacgtg
    18901 ggcgttgtct tcaacggcaa cgaggctctc ctaattgact gcggcgacgt gggcaccttc
    18961 gaatgggcgg cagcagcggg gatcaaggtc aacaaggtcc tggtgacaca ccaccatcgt
    19021 gaacaagtta tggggctact ctcagaagag acgaacgcgg aggtcctcgt ttccgcggca
    19081 gaagaaaagt acttcaccga tgttgctcag tattggctgg cgccgtcctc tcgctggcat
    19141 atttacaatc aacgtcccca tcactggatg ctgccccagt ccgtaaaagt agcgggtgtc
    19201 ctaaaaggcg gtgaatcact cacgtggggg tcggccacca tcaaggtctt agaaaccccc
    19261 gggcacactg atggttcagt aagtttcctg gtcgcggtag atgggctccg tgtggtattt
    19321 tgcggagatc tcctgtatgg gcccgggcag gtccctgatc tgtattgcct ccagaaggga
    19381 cttcatcgtg gccagcggcg aaccagcgac taccacggat ttctgggtgc cagagaaacc
    19441 gtcaaagaga gtctccatcg cgttcttggg caacagcctg atttggtagt cacctccgcc
    19501 ggcggtgtga tcgaaaaacc tgaggtaggt ttgcaacttc tcattgagcg actcgacagc
    19561 ctttatgaca cctacgtttc cacgtccgca ttaaggcact attttccgga gctttttgag
    19621 gagtttcagg ggcgcgcaga ccatctgcca tttcgtccgc agctagcccc acccgcctac
    19681 ctgcgccaca ttggcacaag ctggatcatc cgctcgcagg acggggccgc attcgttatg
    19741 gattgtggca gtccaagggt aattgaggct ctcgaaaaaa tgatcacggc cggtgaaatc
    19801 acgcgcgtgg aaggtttgtg gatcacccat tatcacgacg accatgtgga tgcggtacca
    19861 caattcctcg aaaaattcgc ttgcccttgt tacgccgtgc ggccagtcgc agaaatcatc
    19921 actcgcccca tggcctggcg actaccctgt atttcaccat ccgtagtgcg ggttgaccgt
    19981 gtcttggcgg atggggaatc tttctcctgg catgagttcc gtatgaccgc gttcaatttt
    20041 ccaggccaaa ccctttatca cggagccctg ttcgcggaaa acgcaacgga caagctctta
    20101 tttgtgggtg actccttcac ccctgcgggg attgatgact attgcatcca gaatcggaat
    20161 tttctccatg acggctcggg ttttctgcgc tgcctcgatc tgatcagcca actgaagccc
    20221 acgcatatgt ttaattgtca cgtcgattcg gcctttacat ttcggccaga ggattgtcag
    20281 gcaatcaaag agcgcctcca acaacggcgt gagcttctca aagccatttt gccttggcgc
    20341 gattttcatt tcggcacaga cgagttgtgg gtccgttgcg atccatacga gcaaacggca
    20401 tcaccgggcg acaaggcggt ccttgacctg gtgattctca accacctgac agaagcgctc
    20461 gatgtccacg tacgggtggt cccaccagca agctggtcag gggaaaggtt accaagcgca
    20521 ctgcgaagtt ggcaagattg gcaacaagtc agcatcgccg gtcgcaagga aggacgaatc
    20581 tctcaagaaa tctttgttcc caagtccacg gcaccaggac ggtacgttgt tatggtggat
    20641 atcgccatcc caggcgagac gccgtcgcct cgcatcctat gcggcgttac ggaggccgtg
    20701 attgtgattc aagacgtaag tactaagacc ctattccaca accgcccctc tcccgttcgg
    20761 cgggagaggg gccgggggtg agggtggctc gaatggttac tggttttctc gatcgactcg
    20821 gacgatggct tgccctcacc ctaaccctct cccagcggga gagggaactt tgggataggc
    20881 tctaacacgt cgcggtgata ggcgatttat ttcctttcca gagaacgctt gacttcattc
    20941 tcggatgacc tgtcctcggg aaccattcct tcacagaaag ccggttatgc gcgcgcaata
    21001 agtacacacc tgaccatcac tcccaagggc gccagagacc aatttcctct aaccgccaac
    21061 cggtgagtgt gcccaataga gcacgataac tgcatttgtc gcccggtagt tttgaccctt
    21121 tcgtggttca gttaggagta ttcgctgccg ctttagccaa aatcgctttc gctggagggt
    21181 tttggtacgc ggtatgcatg acagtaaaca agcagaggaa ccaaaccggt ttggaggcag
    21241 gttgagttga cgcggttttt tggaattggc ttgttaaagg agaaggacca tgacttggct
    21301 taagtatgta ttcggcgtaa caattctttt gggtatgacg gcacctgttt gggcacaaaa
    21361 tcaggaagat aaatctcaaa ccccatcggt gacatcaagt gggtccgggg tgctatcccc
    21421 tgctcagcaa cgggccgagt ggttcaagac tatggctgcg attgctgagg agcaagccaa
    21481 gccacagccg gaccaacaaa agattgccgc ccttcagcag aaactctttc agttgcggca
    21541 acaaggtttc gcaccaggat ggcaaccggg aattggtttt caggcaccac ccacgatgat
    21601 gcggccttgc ccgtggggtg gaccgggcct cggaatgggg atgggccgtg gaccaggccc
    21661 tggtggacca agaggccccg gctatggaat gggtgctccg atgggacgcg gttaccgcgg
    21721 gggaccacgg atgtggtaat caccatgcat cgaactgcga cgttatcgtt gcacaggtgc
    21781 gagattccga gttgacaaca tcctgaagat aggccaaaaa agcggtggag cttccggctc
    21841 gaccgctttt tctttttcag aaaagctcga atccattttg tacgttgcag catgaacaaa
    21901 cgccggtgag gcctgacgca tgccaaaaat ctcccaagtt atcgctgttt tcatacttat
    21961 tgccgtcgca gtcatgtttt ccctatggca atggtatgag tatcaagcgg acgtggctgc
    22021 ggcaagagaa gtcctccacg gccacgccga ggggctttgc cgggcgatgg tgggggcaat
    22081 tcgttcccac cgccgattgg gcgcgttttt cgatcaacaa atccaaatta tcctcgacga
    22141 actggttgac tcgcccgatg ttttggccgt tcagatcgcc gatagtgcga aaacgcccat
    22201 cttccgtagc gggtcgagcg acgtcctcac cctgagtgtt gggggcagtt tcgccgatgt
    22261 gggatacgtt tactcggcaa gctttcaact ggcgccgttg gcggccgctg gtccccaggg
    22321 aggggcccgt ggatttgggt ggggtcggtg ggctcgcggc gttgcaccgg aacccgacac
    22381 aagcccgttt gcgtccgggg gattgttcta cgctactgtg gtgcttgatc gaaaaggctt
    22441 tgacaaccat gtccgccgaa ttgccagatc acgggctggg ttatgtttga ctggctggat
    22501 agcattactt ctttttggtt tgaccttccg ctcaagtgtc cgcatggtca aggcagatca
    22561 tgccaagagg ctggccgagt tacaggctcg ccacttcgag gagctaagtc aggcagcgag
    22621 tggcttggct cacgaaaccc gcaatcccct gggactaatt cggggacgag cccaacggat
    22681 tgcgcatcgc gaagacccga catccccaac agcgaccgat gcccggctta tcgtcgagga
    22741 atgcgaccgt ttgaccgccc gcttgacaca gttccttctt tttgccaaac cgttcactct
    22801 tcaacctgtc gaagtcgatg cggacgtggt aatcgaggag gtgctacttc ttttggagcc
    22861 ggattgccag agtaaggatg ttcggtgtgt gcatcacaaa tcgccgcagt cggttcccat
    22921 cgtggcagac cgcgagttga cgcgacaggc actctttaac ctgctccaca acgccgtcaa
    22981 gtacactcgt cctcattccg agatcacagt tatcagccga gtcgatggct caaatgttat
    23041 aatcgagatc atcgaccagg ggccaggtgt tagtcccgaa catattccga aattgtttat
    23101 gccctacttc agtacagatt ctgctggcac gggacttggg ctttcgctgg tgcgaaagat
    23161 tgctgtggcc catgggtggg acatcagcta ttgtccgtcc ccggaaggcg gggccatgtt
    23221 tcgtctgaca atacccctca agaccacagg cagcggcacg cacatcgggg aatttggcca
    23281 caccaggcga taagtaagga ttgttagccc gatatgagcc ctaagacgtc cgatcgttta
    23341 cctacgattt taattgttga cgacgaaagg tcccatcgtg cgctatttcg tgatctcatc
    23401 accaacttag gttttgcggt cgaggaggcc tcgcaggcag aagaagcctt ggaaattgtt
    23461 tctcggcacc cgccgcagct tattctcctc gatgtgcgtc tgccaggcca atccggccta
    23521 gaactcttgc ccgttctcaa agagaaggtc cccacagtgc ccatcatcct catcactgct
    23581 tacggtgatg tccgagaggc ggtggcggcg atgaaaatcg gggccgttga ctacctccta
    23641 aagccggtgg acctggagga actggaggct atcattctcg attatctagg ctgtgagacc
    23701 gcttccgcac cgcgtccggc cgtctccttg cccccattag gcgatgttgt gtgcgtcagc
    23761 ttgggatttc gccgtgtctt ggaaacggca tacctggcag cccgttcgga cattccgatc
    23821 cttattttgg gcccaagtgg gagcggcaaa gaagtcgtcg ctcgactgat ccagcagtgg
    23881 agtcaccgca aagatgggcc ttttattgtg gctaactgtg caggattgcc ggagtcgatt
    23941 gtcgaaagcg aactatttgg gcacaccaaa ggggcgttca caggggccca gacggagagg
    24001 ctgggctatt ttcgcgcggc agacggtggt accctctttt tggatgaaat tggcgaccta
    24061 ccgccgcccg tccaagccaa gctcttgcgg gcgctggaga ccggtgagat tgtaccggtg
    24121 ggaagcgagc gtccgatccg ggtagatttc cgcttgttgg ccgccacgaa ccgcgatctg
    24181 acgaccctcg tgcgagaggg acgatttcgg gaggatttgc tttttcggat caacgccgtc
    24241 gagcttgtcg tgcccccgct gaaggatcgg gtggaagaca tcctcccgct ggcagaattt
    24301 ttcgcgaaac gtttcaccaa acgcgttgtt cgtttctcgc cccaagccga ggcatgcctt
    24361 ttggcatact cttggccagg caatgtgcgg gagctgcgta acgcggttca gcgggcctgc
    24421 ctcctgtccc aaggggaaat cattttgccc gaacatcttc cgccgtcggt ggctttaacc
    24481 tccggcatta cctctgaccg gcaatccacc gccgacgaag gccgcctttc ggccatggag
    24541 cgggcgacta tcctggcgac attggccgaa tgccgcggaa atcgcaccca ggccgccaag
    24601 aaactcggta tcagccgccg tgccttgatt tacaagattc gggcaattga atcccaactg
    24661 aatcattcgt ctagggacaa agactaaggt acgtcccgcc acaaatggcg tgtccaagtc
    24721 cacacaccct tcgcatggca tcttcaacct gccagtgttc gtagtaggct ccctagttcg
    24781 cccaacccac gtccccgatt acggggcttg cgaaaccggg gccgcttgct tctggagctt
    24841 ccgttggcca ggctgtcgcg attgcgcttt aggacgggca ggcggcgtct ccggggcttt
    24901 caggacgggc tttgtggcgg gctgcgtcag gggttcacca tctaatgggg gttggaaacg
    24961 gcttaagtcg aggccaaacc gcgcgagaat ggttggcgct atatcttgac gcatcccatc
    25021 ccgcttaacc aaaggatcgt tggtcgccag gataacatag ggggcataag aatgacgggt
    25081 ctcaccctca ttaaacccat ggtctgccgt cacatacacg agcgtcttgt cgtacaagcc
    25141 caactcttta agtttggcaa tgattttccc tgtccacgta tcgttcgaga tcaaggcatc
    25201 gttgtactct tttgagtttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 ngctggtact gccggcttag cttgggcagc acccggttgt gcggcttgct ttttcgcccc
    25381 ctgtgcctgc ttggcttttg cggccgcctc ttgctgggct tgaatcttag cggcctcttc
    25441 atctgtgagc tgccgcttga acggctcgcg aatctcgccg cagtgttctc tcttgccgat
    25501 gaccgccaca caaacaaaat cgggtccaaa gtgttccttg agtcgttcga agatgctcag
    25561 gcccttgggc acatcccggt agcgaccgtt actgtaaacg cctgtgacct ccgggttata
    25621 gcccgtcaga atctgtgtcc accccgcctt tgtatcggtt gcccccgtca caacgtcgat
    25681 attaaccagc gccccttctt gtgaaagttg ctttaagttg ggcaactcac cgcgctccaa
    25741 acactccatg acgtgggccc gttgggcacc atcccagccg atgaggatga tgttccttgg
    25801 tttcgcggcc gaggcctttt cttgtgcgag gaccggacaa aaggccaaaa gcaccaggct
    25861 caaagccaac acaattttgc gtaacatggc caccactcct ccgcgatctc ttggaaacgc
    25921 catcgaaaaa acaacactga caagccaccg ttccaggagg caggtaccct cccggatcgc
    25981 cgttccctcg gtaactgaat tgcgcggagt ggggtcacat gcacgctaaa aagggattat
    26041 ttgaatcact cgcggcttgg attcgatccc gaatcctggt taaataaaac gtttccaaca
    26101 tgtgctatcc acagcggatg cctcaatcgg aagacggtac ccgtcaagca cgacgcgaaa
    26161 ctttttacag cgaaacaggc ttactcttat tgagaggtag aggttgaagc gatgcaggac
    26221 aattgcggaa acaagaaaga cccgcttcac gataagctcc ttggtggaag gaggattctc
    26281 ccccctcatg tgggatcatg cgaaaccgtt gctgacctga ttgatcgcgt ctttttggcc
    26341 tacaatgccg cccgcttacg cgaagccgcg caattgttca caagaaagat gttgaacgac
    26401 gatgttacga tcggcatgtc actggcaggg gcattaacac ccgccggatt agggatgtca
    26461 tgtgtcgttc ctcttattga agccggattt gtggattgga ttgtggccac cggcgctaat
    26521 ttgtaccacg atttgcactt tgcctacaac tacaagctcg tggcgggcga ctttcggctt
    26581 gacgatcgcc agctccacga ggtgggcgtg gtccgaattt acgatgtcct cctgtctttg
    26641 gacgaatgcc tcatcgaaac tgaccgacgg gtccaggaaa tgctgtccca accagaattc
    26701 cagcggtcca tgacaacggc agaattgcat tacctcatgg gccggtatgc cgcggaagcc
    26761 gaggaaaaga ccggggtggg gcacgtctca atcctggcgg ctgcgtaccg ctgcggggtg
    26821 cccatttata ccagttctcc cggtgactcg acaatcggga tggtcgctgc ggaagtccgg
    26881 ttgcgggggt cacagcttgt gctggatccc ttggcggacg ttaatgagac ggcagccatt
    26941 tattactggt ccaaacaaga ggaccggaaa tccgccgtgg tgattttcgg tggtggaagc
    27001 cccaagaact tccttttaca aacggccccg catgtcaatg acatttcggg tctagaaacc
    27061 cctggccaag actacttcct acaatttacc gatgctcggc ccgatacagg aggactgtcc
    27121 ggggcaacac cgcaagaggc cgttagttgg gggaaagtgg atcccgatca tgtaccggac
    27181 accgtggtgt gttatatcga ttcgacggtt gccatgccgt tattgacggc gtacgccctt
    27241 gcccgacacg cacctcggcc gctgaagcga ctttacgacc gccggcagga acttcttgat
    27301 aatcttcaga aagccataaa ataaagactg acggtggagc cagccttacc aggcttatcg
    27361 aaagcccaag agttcgaccc aatgggcggt aaaagacgaa tcaaagttca tttgacgaga
    27421 ctggggcaat actacgccat cttggccgca ggtatctttt tagcggccat ggtccgtcaa
    27481 gtgaacctcc tgcttctttt ggcaggactg gtagcggcac cgctcgtttt tagcgtgttt
    27541 ctggcaaggc gtaacctttt agcaataaaa gtacgacggc ggctccccca tcgaatttcg
    27601 gcgggcgatc tgctggtggt cgagatcgaa atcgagagaa ccgctcgccg cggccataac
    27661 tggttgctga ccctcaccga ccaggttatc cgaatgggcg aaccagataa cgatcctcgc
    27721 aaccggcaaa agctgtcccc gcaggtcacg tttttcttcc tttgcccggg acgaccccag
    27781 gtggcctcat accgcggccg actggtggaa cgtggccagt acgtgtttgg accgatccga
    27841 gtcaccacgc gctttccgct ggggcttgtt gaagcctcat tggaggtgcc catccggcag
    27901 actcttcttg tttatcctcg tctcggaatc ttgaatttcc ccgcattcca gcgctcgcat
    27961 tttaaccacg aggggcaaac gcggttggaa cgccgctctg acagggcgtc tggagacttt
    28021 tatggtctgc ggcagtggcg gcctggcgac agccctcgac tggtccactg gcgtagctcg
    28081 ctccggcata acgatctatt ggtcagacag ttcgatcgcc cccggcatcg cgatgtggtg
    28141 atcggcctca tccttcgaac gcccgacgat ccaagtccgg gggacctcga gcgggtggaa
    28201 aaggccattc gtttcgtggc aactttggta catgaccttt gtcgccaagg gagtcaccac
    28261 attgcgcttg gcattgccgg aaagaaaacc gagtgggtaa tagggccaag ctcgccgggt
    28321 tttcgcgacc gcgctctgga acttttggcg ctggctgacg ccaatcctca aaatcgcgtg
    28381 gactcgttta tggccgatat tcgtcgccgc gcttcgccgg aaagtgatgt aattacggta
    28441 acgagctacg aggcagaacc gctcgaagat caaatacttg agacaatccc gtctataaag
    28501 ggcgaggcag ggcgtcagga atccgaatcg atgcgatttg tctcgtcgcg cggttggaaa
    28561 ggaacttaca cggtgggcac aaaagagttc gaccttattt tcctagacca attctaagct
    28621 ggttatgagt ccctgactac ttgcaatggc cttgagttca gtacgacgcc tggaactgag
    28681 tctccaattt agtgtcgcat gcatggcgac actggggacc actcttcttg ggctcggaca
    28741 acatagtcaa ctactgcccg cactggcttt agtctcctcc atcgtgagct tcgtgttcac
    28801 tgatcttcta agggtgcttg tcctccgcca atggatgacc aatctcctca tgattattgc
    28861 cctgagcgtg gtggccgttg acctggcccg tgcctcgggc tttgtcaatg tgttggccat
    28921 ggccgatttg ctggtttacc ttcaatgcat tcttcaattc caaacgaaaa acacccgtgt
    28981 gtttcgctac ctggtcacgc taagcgcctt ccaagtcgtt tccgcgagtg cgttttacca
    29041 tggcgtcctt tttggaacgc tgctcatcgt ttatctcctc aatagcctca tcgtgatggt
    29101 gatgctcgcg ttttatgaag tttggcaaaa acaaatggaa catcataagg aagggagtca
    29161 gccgcaaacc gtctcgcatc gcctggtggg tgaccggata cctgattcaa gccctttggg
    29221 gtttaacggc cgactgcttg cacggacatt gttcctgggt gttggttccc ttgcttttgc
    29281 tttggtcgtc ttcatggtcg taccacgcct gagttcccgt ccgtggccgg gactgtcccc
    29341 tgctccccta cggaccgttg gttttgatcg ccaaatcgcc ctgggagagc ttggatttct
    29401 ggtggaagac tcaacacccg tcatgagggt ggaagtcaca gatatggacg gaaaccccgc
    29461 taccgtgatg gcccccgtct atctgcgtgg aggatcctat acgttttacc gcaacggaca
    29521 gtgggaaacg aaccaattta tcgtgggcgg cgaggttcag aatctcaaac ctttgtaccg
    29581 gggtcctggt caaagtctgc accgccagcg gattattctt gaacctttgg atacatccga
    29641 cgttttcggt atctggccgt acggcagatg ggacgatgcc cctttagttt atgacccctt
    29701 gctgacccgt ctatttcgtc tttccgatcg acctagccga atcagttacg aaatgaccac
    29761 gtcggcaatc gagaatgagc gacttgtcga tattgttccg gctaccgagc cagtggcctg
    29821 cgaacaatac ctgcaacttc cctgggttcg agtcgttcct acttggttgg gaccacgttt
    29881 cgaggtggat cgaacagttt atgaattacg gggctgggac cgtgtcgtca agttggcgcg
    29941 tcaatgggcg gcagagtctc cctggcctgc caatgaccat ttccacacag cccgcttctt
    30001 tgaacagcaa ctggcgtatt ccggctatta tcggtactct ttggcggggg tcacgcggaa
    30061 cgccactctt gacccggttg ttgatttcct tacggaacac ccggaaggac actgtgaata
    30121 ctttgccact gccctggctt tactgttgag gtgccaggga atcccaacca ggatcgtgat
    30181 cgggtacaag acgtacgatt acaacaccct cggtcatttc tatcaggtcc gtcaacgcga
    30241 tgcccacgcc tgggtggaag tctatctagc accaaatcag attactcaag acctggttgg
    30301 cgaaaggccc ttggaacttt tccgttttgg ggcatggctt cgccttgatc cgatgccggc
    30361 ctccgcactc ggtttggctt cctcgcaggg tccctggcag cggttctgga acgcttacaa
    30421 cttcctcgat tacatctggc ttaagtatgt catgcaaatg gaccaacccc ggcaggaaac
    30481 ggagatttac cgtcccgtcc gtgagcgggt ccgcaaacag gtcgattggt ttcttgatct
    30541 ccggtggtgg cggcaacagg ttacactggt tgtcgagcaa ctgcgagaaa tacttgccaa
    30601 tcctctgaga ataaccgtcc tcgctattgg gcttgggttt ctcgctcttt tggtgtggaa
    30661 agtcgtccgg atccttcgaa atcctacttg gtcattatgg ctagccaatc ggctacctcg
    30721 acggcttagg gacagccagt tatctgaaca cctggccacc gccgtttaca ggcatttgga
    30781 acatctctta tcgcggcagg gataccgacg tgcaacaggc caaacaccac ggcagtttgc
    30841 cgggagcgtt attcaagccg cccgagcgga taatagtgcg tgcccctggg cggacactgt
    30901 gctctatgct gtctccctgt actatcgact gcgatttgga aatggtctcg cgagaccgcg
    30961 cgactgggat gagctggggc gagtcgttgc cgagctggac cgaacctttt ccaggaaacg
    31021 taccgtacgg agggcaaaaa ccgctcactc ggacggctta aagtaacagc ctatcctaaa
    31081 aagtctccct ctccctccgg gagagggttg gggtgagggc aagcaaatct cggaatcgat
    31141 tgagaaaacc ggcagcgatc cgagccaccc tcgcccccga cccctttccc gccgaactcg
    31201 tgagggggat tatgggatag gccccaagtc catgccgatt ctactctgca gccggttcaa
    31261 tggtggccgt catcgaacca cgacactcag cgaccaacgg atcgcggccg aaagcatgaa
    31321 tctggtcgcg cttcaattcc gcatgctctt ttgtcgtcgt caatacgatg caccgtcccg
    31381 aaagatccac ttctttggcc atctgatagg ccttttcaaa gggatgtcca aaaagctgca
    31441 tgagcatctt gatcacatac tcgtaagtgt gatcatcatc attccaaaga atgacgtgat
    31501 accgagggat gtgacgggta tcggtgcgtt ctcttcgttt ccgacgaggc cgaacctgaa
    31561 tctcgccgac atcttgaccc attgcgtcat ccgccgacat agccagcctc cagggtttca
    31621 acaagtcgac gtcactccag ccggtctagg tttctccctg acctggctgg atagtcggtc
    31681 accccaaaag caattatatt gaagttttcc agttagcgag caggccaaac ggagttgagg
    31741 aaaagccaat atggataacg ccatagcacg ggtgaaagat ttcctagaag cccatcgaac
    31801 cacggcagaa caatttctct cggaattcct cgccatccca agtgtcagtg ccgatcctca
    31861 gcaccgccct gacatgaaac gcgccgccga gtgggtggcc cagtttttct ctcaaatggg
    31921 gcttgccgcg gagctaatcc agtctcccac aggccaccca gtcgtctatg ccgaaacccc
    31981 acattgtgag ggtataccga ccgtgctcat ttacggacat tatgacgtcc agcccgccga
    32041 agatccaacc ctttggcaga caccaccgtt tgagcccact attcgtggtg gtgccattta
    32101 cgcgcgtggt gcaagtgacg ataaggggca ggtgctcacc cacttactgt cggtcggggc
    32161 ttggtaccaa gtgacgaaga gctggccgct taatttcaaa tttgtgatag agggcgaaga
    32221 agaggtgggc agctccgtcc ttgagaagtt cttagatgaa caagcagatc ggcttgcctg
    32281 tgattgtatt gtcattagcg atggaaatca gttcggaccc gaccagcctg cgattaccta
    32341 cgggctgcgt ggaatcgcct atttcgagct acgagttaag ggtcctcatc gggacctcca
    32401 ttctggatcg ttcgggggca cggtcacaaa tccggcaaca gctctgacaa gaatgttggg
    32461 acaattcatg acgcaggacg ggcgagtggc catccccggt ttctatgacg acgttcttcc
    32521 cctaagtaac gaggaacgtc ggcgtttgcg cgaattaccc ttcgacgagc cggctttctg
    32581 ggcctccttg ggtgtggaaa ccgcctgggg agaaccaggc tacaccgttt tggaaaggcg
    32641 ttgggcgcga ccgaccttcg aggtctgtgg cctgtggggt ggttatcaag gggatggtgc
    32701 caaaaccgtc attccggcga acgccggagc aaaaatcagt ttccgactgg ttcctcacca
    32761 agaccccgat cgcattcgcg aggcactgct cagctacctc gcccaaattt gtccgcccgg
    32821 tgttcaatat gaggtcattg acctacacgc ttcaccggcg gtcttgacgt ctttggacag
    32881 cccttacatc aaagccgcgg aaaatgctgt caaaatggcc ttctccaggt cccctgtttt
    32941 cacccgcgaa ggtggttcca ttcctatcgt cagccgcttc caacaacggt tgggggctga
    33001 tgtcctttta ctcggttggg gacaggatga cgacaacact cacgccccaa acgagaagtt
    33061 ttcgcttgct gactttcatc gggggaccct ggcgagtgcc tatctttggg agttcataag
    33121 acaaatcggg aaagcaacgg atagctgaaa gcccctcacg aaatgtacgg ctgctcgctt
    33181 ggaatcgtgg ccaaagtccc aaccaaaccg ttattcccca gctcggactg atgtgaagga
    33241 agtacggaac cggatgtgac atcaggagaa taaaagtatg ctcgacagac gctttattct
    33301 agaaaacacg gatgctgtca aacgaaattg cgagattcgt ggggtggtag cggacgttga
    33361 ccgttttgtc gagctagagc gacagcgcaa agaattacag agtcaactgg atgccatcaa
    33421 tcggcaagcc aatgaggttg ccaaatccat tggacgttgc acaacggcag aagaccggga
    33481 aagcaagaag gaagagggac gtcggctccg ggaacaagca gcacaaattg aaaaacagct
    33541 tcaacagatt gcggcggagt cggactccat tcttaagcag attcccaata tgacccatcc
    33601 cgccgcaccc atcggggatg agacagcttc ccgtgaagtg ggacgcggaa agacgcccat
    33661 tcggcaattc gattttgtgc cgaaggatca cgtccaaatt gctgaggaat tggaccttat
    33721 cgattttgag gccggggccc gtgtgaccgg acatggattc tacttcctca aaaacgaagc
    33781 cgtcctcttg gaattggctc tccagaagtt cgccattgac ctgttgcgtt ccgaaggctt
    33841 cgtggtgaca accacgcccg atctcgcccg aaatgacatc ctggagggca ttggttttct
    33901 gccgcggggg ccggaaactc agatctatag tatcgagaat actgatttga gcctcattgc
    33961 cacggcagag atcacccttg gtgggtatct ggccgaccag gtcctcgaag ccgagcagtt
    34021 acctattaag atggccggta tcagccattg cttccgaacg gaggcggggg ctcatggcag
    34081 ggcaacacgt ggcatctatc gcgtccacca atttaccaag gtcgaaatgt tcgccttcac
    34141 tctgcccgat caaagtgacg acatgctgga gtacttccgg ggcctggagt gcagcatctt
    34201 cgatcaattg ggaattccct atcgggtgat tgacacggct tctggcgacc tcggtggtcc
    34261 agcttaccgg aagtacgatc tggaagcatg gatgcccggt cggggcgaat tcggtgaggt
    34321 tactagcacc tccaattgca cagattacca ggccagacgc ttggggatcc gctatcgcgt
    34381 caagggtgaa aaaggcaccc ggttcgtcca cacgcttaac ggtacggccg tggccatcag
    34441 ccgggcactg attgcgatcc tcgaaaacta tcaacaaaag gatgggtccg ttgtcgttcc
    34501 ggaggtcctt agaccctttg tgggccttga gcggatcggt cctcggcgat cgtagttccg
    34561 cggtgccacg atgacacttc gccacttggc gcgattctgt gatttagagc ttatcccaaa
    34621 aagtccccct ctccctccgg gagagggttg gggtgagggc aagcgaacgt caggctcgat
    34681 tgagaaaacc ggcagcgatt caagtcaccc taacccccga cccctctccc gccgaatggg
    34741 agaggggaga ttatgggata ggcccttcgc ccctcccata aagtctaccc acttttgaga
    34801 ccccaactcg ccgggaaaca gggggttgga cgagaattgt tcgggccgca gcaattgtgg
    34861 cgaattaata acggaggtcc gtttcggaga atcgaccctg cagaaatggg gcctcacaga
    34921 tgcaagtcgc gtcaaaacca caaaataggc aaggcgtgca ttttacccca ggaagcgaac
    34981 atgtgaaggc gaccgcgctc gcaagctcaa accaggactg tgcaccatag ccgcaaaaaa
    35041 gtaactaaaa gttggtaaac tccgagccaa tcccgccttg acgagatgaa acctagggga
    35101 taaagttatt gaaagttgtg gaagacatga gggaaacaat gcacctgatc aattgcgtga
    35161 acctgctgtt gggcttgttg ttattgcctc gccaatgagg ctggccctac ggttcgcgca
    35221 gtagattagg taaaaatgga ctatcgaacc cagggccaag caaaccctgg gttttttgtt
    35281 tgacactaag agtctatccc aaaaagtccc cctctccctg tgggagaggg ttggggtgag
    35341 ggcaagggac tgtcggagtc cagcaagaaa accggcagtg attcgagcca ccctcacccc
    35401 cgacccctct cccgcccaac gggtgagggg agattatgag ataagcttta agcggcaaga
    35461 tggcctccag cgtaattgac cgctaagact cgcagaaggt gaccgtcagg cctgcaaacg
    35521 ttgtaggcag tacgggaaaa ttatggacct cgtgatccgc aaaagcacca taatacagat
    35581 ggaaaaacgg cgattggctc ccactcgggc atggttgtta ttgcgacgac cctacctacc
    35641 gcgtttgtga aggtgtcact ttgagttcgg agtctcttgg cgaatcaggg tcggaaacca
    35701 tgaacgaagc aaatccaaaa gcgcaaacac ggagaattgt tatcttcgat acgacgctcc
    35761 gcgacgggga acaatcgccg ggctgcagta tgaacctcca agaaaagttg gaggtggctc
    35821 acgccttagc acaactgggc gtggatgtta tcgaggccgg tttcccgatt gcctcccccg
    35881 gcgatttcga ggccgtcaag gcgattgccg agtcagtgcg tggtcctgtc atttgcggcc
    35941 ttgcccggtg ccgtccggaa gacatcgacc gcgcttggga agccctccgc tttgccgaat
    36001 ccccgcgcat tcatgtcttc ctcgcgacga gcgccatcca ccgggaacat aagcttcgca
    36061 tgacgaaaga acagatcatc gacgtggccg tggctggggt gcgacgagcc gttggatact
    36121 gccccaacgt tgagttctcg ccggaggatg cagtccgcac ggagatcgat ttcctctgcc
    36181 aggtggtcga ggccgccatt gacgccggtg ccaccacgat taatatcccc gacaccgtag
    36241 ggtacgccac ccccggccaa atgttccggt tgattacaac acttcgggaa cgagttccta
    36301 acatcggaaa ggcggtcatc agcgttcatt gccataacga cctgggcatg gccgttgcca
    36361 atagtctagc tgccgtggag gcgggagcgg gacaggtcga gtgtacgatc aatggcatcg
    36421 gcgagcgggc aggaaattgc gcactcgaag aagttgtcat ggccctccgg gtccgccaag
    36481 acatttacca tgccgacacc gggatcgtga gccaacggct tgtcccaacc agccgactgg
    36541 tggcaggaat taccggcatg gtggtccaac ggaa
//
LOCUS       DAOSUT010000018        34795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_863_length_34795_cov_9.483650, whole genome shotgun sequence.
ACCESSION   DAOSUT010000018 DAOSUT010000000
VERSION     DAOSUT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 34795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34795
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_34795_cov_9.483650"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLD05_02830"
     CDS             complement(<1..1088)
                     /locus_tag="PLD05_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76394.1"
                     /translation="MMRQCLILAGICFLGLGLTEAARSEEGSAERPNVLLIISDDHAW
                     TDYGFMGHPQIKTPSIDKLASESLVFTRGYVPSSLCRPSLATMITGLYPHQHGITGND
                     PALPDPKVNPMAGRANPQFARYYETLMERIEKVPTLPRILGEAGYVSLQTGKWWEGSY
                     RRGGFTEGMTHGDPRRGGRHGDEGLKIGREGLDPIFNFVRRAKGEGKPFFVWYAPMMP
                     HSPHTPPQKILEKYLALNLTPPQARYYAMCEWFDATIGQLLSFLETEGLRENTIIVYL
                     ADNGWIQDPAQPNRFAPKSKRSPYDGGVRTPIIFSWPGRIKPQRDDSSLVSSIDIMPT
                     LLHLCDIPCPQGLPGLNVLDREALSKRRA"
     gene            complement(1193..2389)
                     /locus_tag="PLD05_02835"
     CDS             complement(1193..2389)
                     /locus_tag="PLD05_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76395.1"
                     /translation="MVRKSLDEKLARIHADRSCRDFILADAKDADMAFGLAAPGRSPE
                     HHAAEAKFRTLDEYRQIIREIVAQGLVDIMLMSASTNELLTIRERIFDQSHVTPAVRA
                     NDATDIWLAGGGLGVYDRQPSRPFRTTTIDHMMCGKYECSPEERRLGADLGLYSVTFN
                     NDVELDLRTLEAYKEFRIEAEKKGFRHFLEVFSPNAPGENAPQDIPRFVNDHIVRTLA
                     GVTTAGRPLFLKIPYYGPEAMEALVRYDSSLVVGILGGSAGTTYDAFYMLWEAKKYGA
                     RVALYGRKINNAEDQLTFIKYLRLVADDEIEPAEAVRAYHSDLKKLGIKPYRSLEEDL
                     TLTPTASSYSGQSQTVGRQAAAVERGVRVTMSTWRQPEDDPDFSRMSPKEKALYNIER
                     WKRILG"
     gene            2973..4250
                     /locus_tag="PLD05_02840"
     CDS             2973..4250
                     /locus_tag="PLD05_02840"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOP76396.1"
                     /translation="MSQASNVLVEKAYQLAKERYASWGIDTEKVLTRLQSISISMQCW
                     QGDDVGGFESSGGLSGGGILATGAYPGKARTPSELRADADMAFRLIPGKHRFNLHAIY
                     LESGGVRVERDEIEPNHFRGWMDWAAERGLGLDFNPTFFSHPLAASGFTLTHPDDGVR
                     RFWVRHGQACRRIGEAFGRHLGKPCVTNIWIPDGMKDLPADRKRPRERLVRSLDEILS
                     VPMDPAYHRDAVEGKLFGIGSEAYVAGSHEFYLGYAITRKTLLCLDSGHFHPTESVAD
                     KISSVLLFTEGLLLHVSRGIRWDSDHVVILGDEVQAIANELVAGDYLGRVFIGLDFFD
                     ASINRIAAWVIGMRAMLKALLAALLTPWDHLREFEDSMNYTGRLVLMEAVKTLPMSAV
                     WDYYCLKHDVPLDDAFLSDILRYENEVLSKRGH"
     gene            4430..6823
                     /locus_tag="PLD05_02845"
     CDS             4430..6823
                     /locus_tag="PLD05_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76397.1"
                     /translation="MATLPNTSAKESIFARLDKLNEFERQPVSAEKLHSGRYFAGLFA
                     GEHVAATEFVIGALFVIWGAKAHDILVGLLLGNALAVLSWTLICAPIAVQSRLTLYWY
                     LRQIGGPVLTVVYNLLNAVLYCILAGCMITVAASAVRIPFGIPPQTGVLPTDPGFVVV
                     VIIVGAVVVTLAILGFKRLSQFAVVCSPWMLSMFVCGALIALPSVGHVRNWETFLAVA
                     NERIWTGTGPKVVNVSQQGRALVLELGPIQGHLRVLSEQQAVRGADPLPIGVTVPGFF
                     LSEESDGKAASFVPAEARLEPIYAETPAAPKTAINWKNLTFVSFLENASNENGKVGQQ
                     ASSLAERESAAGESQPSVGEANQERPQQQTRSQRRRGSRASQEGGEQPEESSTALPMP
                     ATPAISGWRIIVESPQVTSPQSVKYVMPKKRAGVICDENFVPLQSFQASITDGPRAMR
                     SPIGFWHIVFFAWICNLAMHLGLSDMAIFRFAKRSWYGLYSAFGMFLGHYIAWICAGI
                     MGAAAARLTMTPLMQLDSGQVAYLILGWAGVVAVVIAGWTTSNPTLYRAGLALQILTP
                     NWPRWVVTLLAGTITTIIACSPFVFIYLLDFVGIYGILLMPVGAIVVVEHWVFPLLGW
                     RRFWATYRGQWLNIPAILAWGVGLGLALVCWKFQWLHLFFLVIPVWFATALTYLILAG
                     LGGAARKTEGETSPTTPSSDSAGRESTPASTFAGEKKNLDPGTDTPGRRPLLMYAFGF
                     AAMVCLLGSYAFAWFVFAGYSTAEDVKPTLLYLALAYFVSGVIYLSLRERRTATSAA"
     gene            6879..>9835
                     /locus_tag="PLD05_02850"
     CDS             6879..>9835
                     /locus_tag="PLD05_02850"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOP76398.1"
                     /translation="MRRSIVVVCCSVFLALHSVGLMSGEELSPARVTYLRCEYRVNPL
                     GIGTTRPRLSWIIEDPRTGAAQTAYQIIVASSSEILAENRGDLWDSGKVISSEMNQIE
                     YAGKPLQSRMKCYWKVRIWDANQQATPFSEPAMWSMGLLDPQEMKARWIQASQPLVHP
                     AKGTQLDFGDCPWVWYPEENTDPREKAPAGPRYFRKVIELPSEKAIKRARLLLTADDS
                     FELFCNGQPAGRGAGYNAPQIADLTNRLQPGRNTLAIVATNASPSPAGVCGRLRVEFE
                     SGESSEWLIDSTWKAFPRAVAGWNQVDFDDSAWPFAVVVGKMGDKPWGAISAQGLDIF
                     GCGLFRKAFRIDKPIQSATLYMSALGVGEIHINGQLVNDEYFAPGWTDYRKRVYYNTY
                     DVTNLVRQGDNAIGAILAAGWYAGAIGWKSERFHFGRDPKLWVQLEIVTSDGERMTIV
                     SDRTWKYTFGPHVEAEFLAGETYDARLEIPNWSLPGLDESQWQPVQEANDVQVLLEAQ
                     RGVPVIRTGELPAKSVSEPKPGVFVFDLGQNFAGFARLKVRGPKGTAVQLRFAEMLNP
                     DGTIYTTNLRSARATDTYILKGEGEEVWEPRFTFHGFRYVEVTGYPGRPPLDAITGIA
                     INSDIPLVGSFRCSSEMVNQLYSNIVWTQRANFISVPTDCPQRDERLGWTGDAQVFIR
                     AATYNADVAAFFNKWLVDLEDAQGPEGDFPDVAPRVVALGGGTAAWADAGVICPWTIY
                     LVYHDRRVLEEHFDAMERWIAYCQKHSQNLLRPAAGYGDWLSIKADTPKDVLATAFFA
                     YSTHLTAECARALGLAEKAAYYDKLFEEIRAAFQKAYVSPDGRVKGDTQTCYVLALYF
                     NLLPEALRPKAAQYLVEDIRKRDTHLSTGFVGTAYLMPVLSETGNTPLAYRLLLNDTF
                     PSWGFSIRHGATSIWERWDGWTPEKGFQDPGMNSFAHYSFGAVGRWMFQYVAGIDTDG
                     PGFRRLILKPHVA"
     assembly_gap    9836..9935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9981..10799
                     /gene="proC"
                     /locus_tag="PLD05_02855"
     CDS             9981..10799
                     /gene="proC"
                     /locus_tag="PLD05_02855"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOP76399.1"
                     /translation="MLNKRIGMIGAGQMALALAAGFVRKGLVPPEYILASDPLENARQ
                     RFQEVTGARVTDNNLEVVRTQDIIILAIKPQVISKVFEELQGRISPEKLVISIVAGVT
                     LETLQRALGENARIIRAMPNTPALVGSGITAYAVGKNIQPADVQTVEAILSAVGVAVQ
                     VEERLMNAVTAVSGSGPAFVYLFIEALVDAGVRHGLPRELARQLVVHTVRGASEMVIQ
                     SGEHPALLRDRVTSPGGTTIAGLAALETNGFRGAVFAAIEAAVQRAEELSRGSR"
     gene            10895..>12111
                     /locus_tag="PLD05_02860"
     CDS             10895..>12111
                     /locus_tag="PLD05_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP76400.1"
                     /translation="MSSVEYSHNGPRNVIICLICAIALVFGATGFGQEVCRQSARAIP
                     IVADVDVAVIGGSTGAVAAAVSAANEGARVFLAAPQPYLGDDMTATLRLWLEPDEKPT
                     TDLARAVYSDPLRPAPEANRARYGFRYQLDVPSDPKHRDTSPPSMLTDGKFMHAPTES
                     VQYNSDATITADLETPQVVDRVNLIFFWRPSGEPGVGFKVAGIEVSVSDSGKDWVSIG
                     KADVTPPQAASGDPPLSVRVDVGQKKARFVKVFVRKAPDVERMLLGELEILGPAQEPV
                     EREQAKYLPPRPLHVKQLLDKVLLDANVQYLYSCYPTDVVRDASGRVCGVVIANRAGR
                     QVVLSKTIIDATDRATVARLAGAKFSKYPSGTHVFTRVVIGGEPVTGEGLEHRVIDPP
                     FVGQFPNRARTSSG"
     assembly_gap    12112..12211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12212..14239
                     /locus_tag="PLD05_02865"
     CDS             <12212..14239
                     /locus_tag="PLD05_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP76401.1"
                     /translation="VWILGGCAAVSREQAEKLLRPVHLMELGERLGREIARIASSTPK
                     PESPVVAVNPIQGNPIAGDVGEFLYGLRPVKKYPTIPQPETTIPVLGKYDVVVIGGGT
                     GGAAAGIGAARQGAKTLVVEYLTTLGGVGTAGAISSYYWGNRTGFTATVDEGRASWVI
                     EQKAEWWRSEILKAGGEIWMNCLGCGAYKDNGRVKGAIVVTPFGRGVVLADVVIDSTG
                     NADIAAAAGAETMYVDDTEPAMQGTGLPPRELGARYTNTDFTITDETDLMDVWHVFIY
                     SKLKYSSAFDQGRLVDTRERRRIVGEFTMTLLDQVLGRTYPDTICVAYSNFDTHGFTI
                     DPYLELEHPDRKGLRVNIPYRCLIPKGLEGLLVTGLGVSAHRDAIPLIRMQADIQNQG
                     YAAGVAAAWLAQQDLPVRQLDVRKLQQHLVEMGNLPESVLKEEDSFPLPDSVVAEAVA
                     RLKNNYDGAAVVLAHSEIALPKLREAYAKAQTDEERVIYAHVLAVLGDNTGLETLMAE
                     VRKFPEWDKGWNYQGMGQFGRALSRLDELIIALGRTRDPRAVPVILEKVRLLTPESEF
                     SHFRAVALALEMIGDRSAARRLAELLTMPGMSGYVHRTVQDAIRFDQSSPGGTNAVQT
                     RRDSIRELSLARALYRCGDYGGIGEQILRAYTEDLRGHLARHAQAVLNKGR"
     gene            complement(14297..16750)
                     /locus_tag="PLD05_02870"
     CDS             complement(14297..16750)
                     /locus_tag="PLD05_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76402.1"
                     /translation="MSLNEDARRSSGGAGNENQDADARLVELTELRATRLGFPFVDVR
                     KVQPSQEALDSVPARVAREYGLLPLERKSDHLLVAVSEPLLPEQSDRLRFTLDCPVRT
                     VLAVKEHLLKAIAKWYGEEKDAQEAESPSTGEGKESEKGAVEFIQVEPEESQAATEVV
                     IDPDSPEVTRAIQTIVGEGIRLGASRVALVSYHGVTKVGFRVEDGICSRNDLPGELLA
                     PIAARLIGMTNFYGTIKVVVRGTERRLKASCRTTPYGLALVIEFDKDESAIQAARERA
                     SLLSYPFTELEKPQIPADVLATIPRDIARTYYVLPLSVTNDVVAIAMAEPPGPQLQDE
                     LRFALGRPFQVMLAPEGRLRAAIERFYGPAEPEAVTVLLAELARPMTTAEVAGRLATW
                     ERPSPPQGHPAEAIFGYLRQMATEKLLELFEDIRRRPKLCIRSSKRWQLEVVIPRADL
                     FSVLPQAYRSYLENRLWLVREAIIARIFNIIVRHPEARHLAIAYALYVAACRLAEGER
                     NVPIDPVMLIDEWFNFLYCYALKTNPKIETNAALLNYLSNQSEELVRSIVGVTRDPSL
                     VQNIELAPRWLEALAQQTLVQDVIDETCGTAEAMLNLFVAEARHYRASHMLLLPHPEY
                     LEVAYRIQNRILSTVRVPACWFLPILSRLLANLVNHSEWQVTVAGKTIQAQVRLHVAA
                     EGLAALIEFMADSRQVEVAQMLAASTDCRFVRLHDVQVPEDILKLVDGGVARHLCVLP
                     IATDGETIILAVSQPPSSRHMQELQILLARPIQAVIAPEDELLAAIYRSYPPPYRYEP
                     SAAARYFFQRYTGLLNPVT"
     gene            complement(17360..17785)
                     /locus_tag="PLD05_02875"
     CDS             complement(17360..17785)
                     /locus_tag="PLD05_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76403.1"
                     /translation="MRFEKAVVFTLVFAIVYAVGVGCQKAGEKPQATKENQTRQMEAT
                     NQDWAGGPASTIEGEPGTATVQQATSDQSAVAEAAKAGDAGVGIQESAASEDDGGISL
                     GRMVGGVFRAARSVVPLPVSASPDRRTEPDDQAPAFPQQ"
     gene            complement(17919..18575)
                     /locus_tag="PLD05_02880"
     CDS             complement(17919..18575)
                     /locus_tag="PLD05_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP76404.1"
                     /translation="MSDDSPENRHVTRRGIPTAADWDLAYREGLPPWEMGCPSPELVA
                     FLDKGLLQKASVLELGCGTGADAVFMAQRRLDVTAVDFAPTAIERARTRAQRAGVNVC
                     FVLDDVFHFAYRTQTFDFIYDCAFYQYIRQWELSRLLDLLWRVTKPGSYYLCITYLGG
                     ESLVGIPPVSEDSIRWELGRLFEILELKQFNFYSALRRHDVSGLSVLMQRPLAKGTAH
                     "
     gene            19194..20315
                     /gene="pheA"
                     /locus_tag="PLD05_02885"
     CDS             19194..20315
                     /gene="pheA"
                     /locus_tag="PLD05_02885"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOP76405.1"
                     /translation="MPKGARTAKAFRLPAKPTVAKLQQALADLDQRLLELCNLRAEMS
                     VQLSQLRQEEGQPDFQLSDEDRQLAAVVEQNKGPLPPAAIKAVFRELISACRAMIKPL
                     RVAYLGPQYSYSHLAAIHRFGQQVDYVAVGSIAAVFEEVTRGHCQFGLVPLENSTDGR
                     IADTLDMFIRTPIRICGQVELEIHHSLLSKSSRSEIREVYSKPQALSQCRNWLAKHLP
                     GARLIEVTSTSTAAQLAAEKPGAAAIASIQAGIHYGLNTLAENIEDNPGNMTRFAVIG
                     NVICPRTGHDKTALMFQVEHRPGALAEAMNIFKRNRLNLTWIESFPVPGCRGAYYFFA
                     ELEGHESEPRVRRALASLSKKTQRLELLGSYPIPTPVST"
     gene            20452..20964
                     /locus_tag="PLD05_02890"
     CDS             20452..20964
                     /locus_tag="PLD05_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOP76406.1"
                     /translation="MKSETQAKESAPHLLRVISGGQTGVDRAALDAAIQLNIPHGGWC
                     PLGRLAEDGKIPDCYQLRETHSADYAVRTEWNVRDSDATLILTKGELSGGTRLTAIFA
                     LRYSKPLRVVNLEDNLPPKSVVDWLLSHNVRTLNVAGPRESTCPEIYQMAKAYLCDLL
                     QEWQRRCSQA"
     gene            complement(21019..21810)
                     /locus_tag="PLD05_02895"
     CDS             complement(21019..21810)
                     /locus_tag="PLD05_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOP76407.1"
                     /translation="MTPTQSGQSDSQLRVADRVVPQTNPVVEHPLMPAIRWAYQGLQD
                     LQKIQDYSAIMVKRERVNGKLNDPEYAFVKIRHKPFSVYMHFLEPASVKGQEVIYVEG
                     QNDGKMWAHPAGIKNKLIGTVSLDPTGPIAMQNNRYPITEIGILNLVRRLIEVGEQDM
                     KYGECEVKFFEGAKINDRTCTCIQVIHPVPRRNFRFHLARIYVDTEHNLPVRYESYDW
                     PTEPDGAPQLIEEYTYLNIKVNNGFTDLDFDIRNPNYAFRVKENQ"
     gene            22249..22905
                     /locus_tag="PLD05_02900"
     CDS             22249..22905
                     /locus_tag="PLD05_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP76408.1"
                     /translation="MADRTHPRVWTIVSAMFDQNCYVVANDDCQECVIIDPGLEPGKI
                     IRSISAQGLIPRAILITHGHADHIAGIEGVWRRWPEIEIVVGEQDAEKLTNPRLNLSA
                     DFGFPLTTPPPTKLVRDGDQLTIAGLEILVREVPGHSAGHVVYVVNQATPPLAFVGDV
                     IFAGSVGRTDFPGGSMEQLAEGIRSKIYSLPEETELWPGHGEPTTVGRERRTNPFVRG
                     "
     gene            complement(23010..23216)
                     /locus_tag="PLD05_02905"
     CDS             complement(23010..23216)
                     /locus_tag="PLD05_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOP76409.1"
                     /translation="MADAVYKKIQVVGTSSESFSDAAAKAVSKVAETEKNLSWFEVVE
                     LRGAISDGKITQYQVTVNVGCRVS"
     gene            23374..24690
                     /locus_tag="PLD05_02910"
     CDS             23374..24690
                     /locus_tag="PLD05_02910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOP76410.1"
                     /translation="MSGEQRRVVITGLGVICPIGTGATEIWQAVTNRQSGVSSPSCLP
                     TETLPIKAAGEARQFTGNIKDFGEMDKESQKNLRKSLKVMCRECQMGVAAAQLALNDA
                     SLTQGSFDPERVGTSFGSDYMVSSAEEFTEGIRACLDGNGVFEFHRWASDGMPRLFPL
                     WLLKYLPNMPASHVAIYNDLRGPSNSITMREASPAICVRDAMHAIQTDREDAAVVGVT
                     GTRLSLMKAMHAIQQEEVAIYDGEPQRACRPFDLHRSGMVPGEGAAAIILEELKTATR
                     RGASIYGEVLAASSAAVLERKCVARRREAMRQAMLNAIHEAGLKPTDIGFVMAHGLGT
                     QTCDVEEAQALSDVFGSRGVPIIAAKSYFGNLGAASGLVEMILGILALRHRQLFATLN
                     YETPDPACPVDVVTEPRPIEKMVFMNVNVTPQGQASAAIIAAYDGT"
     gene            24801..>25051
                     /locus_tag="PLD05_02915"
     CDS             24801..>25051
                     /locus_tag="PLD05_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76411.1"
                     /translation="MSSSDQSIIIRGARVHNLKGINLDIPKDRIVVICGPSGSGKTSL
                     AFDTLFVEGQRRYIESFSPRIRQRLDDLPPSEVDHISGLS"
     assembly_gap    25052..25151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25152..27643
                     /locus_tag="PLD05_02920"
     CDS             <25152..27643
                     /locus_tag="PLD05_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOP76412.1"
                     /translation="SKAATLTSVLADLRASGWARVIVGNRIVNLTESSLAEVEKQLVG
                     GESQRSEGFDKLNDHRSVYVVADRLTIQEKKVPGRFRETLDRLENEGKDVVTLFIQGN
                     EPLVTSSTAGAQAVTMIIEGERWIRYDFPLALCCATCHIDYELPHPDLFNFNTAAGAC
                     ETCQGLGTVDSLEASQIFPDRHLSLKDGAAAPFRSASFSRFHLRLLEFAKRIRIPIDR
                     PVGDLPQEYIDWLLHGSPDDSSSCIEQIFRELERYRYKPQIKTFLSRWRVPRTCPNCQ
                     GSRLRIQSLAVRLVPKTAMENFFGKGMTPPSPLDQTEKPLNIAEFCEKSIGDARAFLK
                     TVVPNGLNPGARLAVNEVLRRLQMLESLGLGYLTLSRPADTLSQGEFRRVLLSAALGS
                     SLVNVLYILDEPSLGLHPNDIHRLRDVITLLRDRGNTVVVVEHDEALLKCADWVIELG
                     PGAGERGGEVVFQGTLEDLQKSETLTGEYLSARRQIKIDRTRRTPAGWIKLVGATGHN
                     LKHVTAEFPLNVLCVVTGVSGAGKSSLIEETLYPALCQRLFGTSSPALPFEDLIGERQ
                     ISDVILVDQSPISKTSRSNPATILKVFDEIRALFAQTSEAKTRGLTPGHFSFNAGDGR
                     CGRCLGEGRLEIDMEFLPNMEITCPDCHGRRYRPDVLSVLYRGKSIADVLDLTIREAY
                     FFFRGHATIQSKLRRLMDVGLEYLRLGQATRTLSGGESQRLRLASYFTRSHKKATLFI
                     LNEPTTGLHPHDIVQLLRCFESLLAYGHSLIVIEHNLHVMVAADYIIDLGPGPAEQGG
                     SIVAQGPPEEIARTPESVTGRYLLDLLPQMS"
     gene            27730..28764
                     /locus_tag="PLD05_02925"
     CDS             27730..28764
                     /locus_tag="PLD05_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOP76413.1"
                     /translation="MSQLHLNRYIRQVQCAKFGEESQQRLQTSRVFICGCGALGTTLA
                     NHLVRAGVGFVRLVDRDLVNLHNLHRQVLFDEADAAQSLPKAIAAVEHLKRINSEVSL
                     EAIVADVRPNNIDQFAFDVDLILDATDNFETRFLLNDYSLKHKIPWIFAACLSTQGQV
                     YPIIPGRTPCLRCLVPELPLPGSVPTCEMAGILGPVVGVVASIQAMEAMKILAKKFDS
                     VVPDLIAIDLWPFRIQQIKVQSLTTQECPACHLGQYDYLSTVGLSLTTELCGQKSVQI
                     TPPCPAKCDLDHLSQQWSTLGTVERSPFFLRLRSAGWVLTLFPDGRAIIDGINDVALA
                     RSIYAQFVGM"
     gene            complement(28818..30680)
                     /locus_tag="PLD05_02930"
     CDS             complement(28818..30680)
                     /locus_tag="PLD05_02930"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOP76414.1"
                     /translation="MVCPPVVPLRVLRSIATVATVLFVLLSIAKTQAENVLTTLRREH
                     PRLIVLRTDEERVKKLLSSDPMAREIFQSIQREAESTLKQEPIRHRLIGPRLLDQSRR
                     CLSRMYLLGTSYRLTGDKRFADRAIREMLTAADFPDWNPSHFLDTAEMTHALAIGYDW
                     LYAVMTPEQRQKIREAIVEKGLKEGEKVYRKGTWWTKTPWNWNQVCNGGMTIGALAIA
                     DEEPQLAQYIVEQAVQSLPHAMANYAPDGAWAEGPGYWSYATHYTVYMLAALETALGT
                     DFGLSNQPGFDRAGDFRIHVIGPTRRAFNFADGGDRVGSAPIMLWLAAKFHKPLYAWH
                     ELYYVGSPQSPLHLWWYFKPVGNLDKEPTTRWFRNSDVVMMRTDWSDQAIYVGFKGGD
                     NSVNHSHLELGSFVLDGLGERWVLDLGPDDYNLPGYFGSQRWTYYRLGTEGQNTLLFD
                     GQNQNPRAKAPVLACYDTGVRKHAVVDLTAAYNPGNETASAKDSVQRGIAILGKDWVV
                     VQDEIMGHAGQTVQWQIHTEAKVQVRGKVAELALGGKTVKVFLLSPEKAVFQTLSANP
                     SRPQRQNPGITRLAVQTPVPQNGMTIAVAFQFNPSAKAEAPKIVPLKEWPGQIR"
     gene            31233..31697
                     /locus_tag="PLD05_02935"
     CDS             31233..31697
                     /locus_tag="PLD05_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76415.1"
                     /translation="MNRLSILSLAAVSLVIVAALAASAGPCDQPCAPPACQPACEQPA
                     CEPACEPACVRVGLVARLRAWCEARRAAAACCKPEACEPEACKPACEPACEPTCEKPA
                     RCCGLLARIRARRAAVECCEVAPAAEQPAPAASPAEAAPAAPQPAPAPAPAT"
     gene            32021..32485
                     /gene="nrdR"
                     /locus_tag="PLD05_02940"
     CDS             32021..32485
                     /gene="nrdR"
                     /locus_tag="PLD05_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOP76416.1"
                     /translation="MKCPYCREDDDRVTDSRIAENGYAIRRRRQCNRCGKRFTTYERV
                     ERRRLKVVKKDGSRVPYDRNKIKTGLEKACWKRPISDEQIESIVTAIEDDIEKNFENE
                     VESRYIGELAMKYLRELDQVAYVRFASVYRQFEDVHDFMEEVRPMLDQARSG"
     gene            complement(32600..>34795)
                     /locus_tag="PLD05_02945"
     CDS             complement(32600..>34795)
                     /locus_tag="PLD05_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOP76417.1"
                     /translation="EQDLDFSEVITNAYQPKEGEESPVVNIIGPRTEVVGKKLLSPIS
                     DHGQAALIILLLKGEIMSVENMKPLLAIYEKLDELRRSPQKPPGLELGVTGSGAVGTE
                     MLIAAEESIRNTEVATIGLIIIILLVVYRAPLLAVVPLLAIAAAVFTAFDLLAIIAGI
                     SLKTNAFEFAVFKTTKIFVVVVLFGSGTDYCLFLISRYKEELSKGLGTIQAAAKALDG
                     VSAALFASAWTTILGLGMMIFSDFGKFRNGGPAIAVALIVALAACLTLAPAILCVLGR
                     KIFWPFRVDLSPRDDTDDISLGKRGILGNYSQGLWNRLAGGVLKAPGLILAGSLLVMA
                     PFVWQAWEVDLTYDLLSELGPDRECVKGTNLLREYFPAGETGPVTVLVYHPDANFNEG
                     PDRYRRLPELVSTIYAMEYTDSAGHRIEPIQTVRSLVDPLGEKSGRSGLFGSLRRGVI
                     SGRAKTMYVSQTGPYQGKVARLDVVFPYDPFSIESIRLLDALENYLNEWAAKNPSWQG
                     AAFYFTGTTPGIRDLRAVTLSDQKLIQQLVPIAVLFVLILILRKPVLSIFLVLSVIWG
                     YYVTLGVTELLFAWLHGATYHGLDWKLPTFLFVILVAVGEDYNIYLVTRVVEEQARFG
                     FEEGLRSALRKTGGIITSCGIIMAGTFIAMISGSLRTMHQLGFALAMGVLLDTLVIRT
                     ILVPCVIVLLHKMSRQWRRKEGPPKGESLPLAQQLEPHIENQVPAKTLP"
BASE COUNT         7770 a         9544 c         9342 g         7839 t
ORIGIN      
        1 gcgcggcgtt tgctgagggc ctcacgatcg aggacattca agccgggaag cccctgggga
       61 catggaatgt cgcacaagtg caacaacgtc ggcataatgt cgatactgct caccaagctc
      121 gagtcatctc gttgaggctt gattcgcccc ggccaagaaa agatgattgg tgttcgcacg
      181 cctccgtcgt aaggtgatcg cttagatttg ggcgcgaatc gattgggctg agctggatcc
      241 tgtatccagc cgttgtctgc cagataaacg atgatggtat tttcgcgaag gccctcggtt
      301 tccaaaaagc ttagcaattg tccgatcgtg gcgtcaaacc attcgcacat ggcataatac
      361 cgggcctgag gaggggtgag atttagtgcc aagtactttt ccaaaatctt ctggggtggt
      421 gtgtgtggac tgtggggcat cataggtgcg taccaaacga agaacggttt gccctctccc
      481 tttgcccgtc gcacaaagtt gaaaatgggg tccaacccct ctcggccgat cttgagacct
      541 tcgtcaccgt gtcgaccacc acgacgcgga tcaccatgcg tcataccctc ggtaaaaccg
      601 ccacgtcgat aagatccttc ccaccattta ccggtctgaa gcgagacgta accagcctca
      661 cccaggattc ggggcaacgt gggtaccttt tctatccttt ccataagcgt ttcataatac
      721 cttgcgaact gtggattggc ccgtcctgcc atgggattca ccttaggatc aggcagagca
      781 ggatcattcc ctgtaatacc atgttgatgg gggtaaagcc ccgtgatcat tgttgctagc
      841 gagggacggc atagcgagct gggcacgtac ccacgggtaa ataccaggga ctcgctggcc
      901 aacttgtcga ttgagggtgt tttgatctgt gggtgcccca tgaaaccata gtccgtccag
      961 gcgtggtcgt cggaaatgat gagcaagaca ttaggacgct cggccgaacc ctcttcagat
     1021 cgggcagcct ccgttaaacc caatcccaag aaacatattc ccgccagaat caagcactgt
     1081 cgcatcataa aatacctccc acacggttaa aagacattcc tacgccactc tggggacaca
     1141 tggtgggttt ctctgcagaa cccaggtcag gtcaattgct gcccacgggg catcatccca
     1201 agatccgttt ccatcgttca atattgtaaa gggccttctc tttaggagac atccggctga
     1261 agtcaggatc atcctccggc tgccgccacg tggacatcgt gacgcgaacc cctcgttcaa
     1321 ccgctgctgc ctgacgacca actgtttgac tttgccccga ataactgctg gctgtcggag
     1381 ttagcgtcaa atcctcctct agcgagcggt acggcttgat acccaacttc ttcaagtcgc
     1441 tgtgatacgc tcgcacagcc tctgcgggtt caatttcatc gtctgcaacc agacgaaggt
     1501 acttgataaa cgtcagttgg tcttccgcat tattgatctt tcgaccgtaa agggccaccc
     1561 gtgccccgta ctttttggct tcccacagca tgtagaacgc atcgtatgtg gttcccgccg
     1621 atccaccaag gataccgacc acgagcgagc tatcataccg gaccaaggcc tccatagctt
     1681 ctggcccata gtaagggatt ttcagaaaaa ggggacgccc agcagttgtg acgccggcga
     1741 gagttcgcac aatatggtca ttgacaaacc gcgggatgtc ttggggagca ttttcgcccg
     1801 gtgcgtttgg gctgaatact tcgagaaaat gccggaatcc cttcttttcg gcttcgatgc
     1861 gaaactcctt gtacgcctcc agcgttctca aatcaagttc gacgtcgttg ttaaatgtga
     1921 ccgaataaag ccccaggtcc gccccgagtc ttctttcctc cgggctgcac tcatactttc
     1981 cgcacatcat gtgatcgatt gtggtcgtgc gaaacggccg cgatggttgt cggtcataga
     2041 cgcccaaccc acccccagcg agccaaatgt cggtcgcatc gttcgcccgg actgctggag
     2101 tgacgtggct ttgatcgaaa atcctttccc gtatcgtcag cagttcattg gtgctggcgc
     2161 tcatcagcat gatatcgacc aagccctgcg ccacaatttc ccgaatgatt tggcgatact
     2221 cgtcgagtgt tcgaaacttc gcctctgcgg catggtgttc cggactgcgt cccggggctg
     2281 ccagaccaaa agccatatcg gcgtctttgg catctgccaa gatgaaatcc cggcaggaac
     2341 ggtccgcatg aattcgagcc agtttttcgt ccagggattt tctcaccatg acatgtgcct
     2401 cccaagaggc ttgatctgcc ggccttggaa tcaaagccgc gtatgtccgt ttgtgttcac
     2461 aatcgacatt ttacacaaaa cccttcttaa ccatcgacca taacggtcgg cctgtcaagt
     2521 ttcctcaact ggcgccgcgt gttgcatcac actcacttga ctgccgaagg attaccccct
     2581 ccggtcaccg acctgagact ctccaggtca tcacgacccc ttggatttga ggatgcggtc
     2641 ggtagtgaaa ggccttttgc caacccaaac ccactgaaat tctccgcgcc gcgaaaacat
     2701 tttggaacca tccgcacaac cgtcatttca atggaaatcc acatcatcgg ttctaaccgc
     2761 acttgcctgc cacgccacga acagtttgtt ttcgcgaaag gaagaaggaa tttgaatctg
     2821 ctatccgatc cggaaccgcg ttcctccttg ggaagctcgt tcagagaccc cctggttcct
     2881 cggcatccga tgtatcacaa taatcacatc tttttgtcag tcgaatgcta attcttttgt
     2941 cacgggaccg gagacgttgt gaggaacggg tcatgagcca agcgtcgaat gttctcgtag
     3001 aaaaggctta tcagttagcc aaagaacgat atgcctcctg gggaatcgat accgaaaagg
     3061 tccttactcg cctacaatcc atctccattt ccatgcagtg ttggcaaggg gatgacgtgg
     3121 gtggatttga gagttcaggc ggactctcgg gcgggggaat cctggccaca ggagcttatc
     3181 ccggcaaggc ccgcacgccc tcggagctgc gtgctgacgc cgacatggcc tttcggttga
     3241 ttccaggcaa acaccggttt aacctccatg cgatttatct cgaatcgggg ggtgtgcggg
     3301 tagaaaggga cgagattgaa ccgaaccatt tccgcggatg gatggattgg gcggcagaac
     3361 gcggtttggg gcttgacttc aatcccacct tcttttcaca cccattggca gccagtggat
     3421 tcactcttac ccacccggat gatggcgtgc ggcgattttg ggtccgtcat gggcaggctt
     3481 gccgccggat cggcgaggca tttggaaggc accttggcaa gccatgcgtt acgaacattt
     3541 ggattccgga tggcatgaag gacttgcctg ccgatagaaa acggccaagg gaacggctgg
     3601 tgcggtccct cgatgagatc ctgtccgttc ccatggatcc tgcctaccac cgcgatgccg
     3661 ttgaagggaa actgtttggc atcggttcgg aggcctatgt cgctgggtcg cacgagtttt
     3721 acctagggta tgctatcacc cggaagacac tcctctgctt ggactccgga cattttcatc
     3781 ccacggaaag cgtcgctgac aagatttcct ctgtcctttt gtttacagag ggcctcctcc
     3841 tccacgtgag ccgcggcatt cggtgggaca gcgaccacgt ggtcatcctc ggcgacgagg
     3901 ttcaggctat tgctaacgag ctcgttgcgg gagattatct gggccgcgtc tttatcggtc
     3961 ttgacttctt cgacgccagt atcaaccgga ttgcagcttg ggtcattggc atgcgggcca
     4021 tgctcaaagc gcttctggct gccctgttga cgccctggga ccacctccga gagtttgaag
     4081 actctatgaa ctacaccggg cgtctggtcc tgatggaggc tgtcaagact ctgccgatgt
     4141 cggcggtttg ggattattac tgtttgaaac acgatgtccc ccttgatgat gccttcctgt
     4201 cggacattct ccgctacgag aatgaggtcc tatcgaagcg gggacattga agccaaagag
     4261 gatttctcgc tctgaaaatg atgaggcgaa aaggcttact cgcacggtga tgggtttttg
     4321 tgcattttct gatggatttc gcgcacgcgc ttgacaatag tcacccaagg tcctcatact
     4381 ttgcagcagg tttggctcgg cgaaaggttt tcagtgaaag gttctgacga tggccactct
     4441 gcccaatacc tcggcgaagg aatcgatttt tgcccgtcta gataaattga atgaattcga
     4501 acgccagccc gtgagtgccg agaaattgca cagtgggcgc tatttcgcag gcctttttgc
     4561 cggcgaacac gtggcggcta ccgaattcgt gatcggtgcg ctcttcgtca tttggggggc
     4621 aaaggcccat gacattctcg tcgggctact tctagggaat gcgctagcag tcctctcgtg
     4681 gaccctgatt tgtgcaccga ttgcggtcca atcgcggtta acgctgtatt ggtacctgcg
     4741 acaaatcggt ggaccggtgc tcacggtcgt atataacttg ctcaacgccg tgctgtactg
     4801 cattttggca ggatgcatga tcacggtggc agcttccgcc gtccgaattc cttttggaat
     4861 cccgccacaa acgggggttc ttccgaccga ccctggattt gtagtggtcg tcatcatcgt
     4921 cggtgccgta gtcgttactc tggccattct cggttttaag cgtctctctc agtttgcggt
     4981 tgtttgttca ccatggatgc taagcatgtt cgtttgtggg gccttgatcg cattgcccag
     5041 tgtggggcac gttcgtaact gggaaacgtt ccttgccgtc gcaaacgaaa gaatctggac
     5101 gggaaccgga ccgaaagtgg ttaacgtatc gcagcaagga cgggcccttg ttttggagct
     5161 tggaccaatc caggggcatt tgcgggttct tagcgaacag caggcggtaa ggggggcaga
     5221 cccattaccc attggtgtca ctgtacccgg tttcttcttg tcggaagaaa gtgacggaaa
     5281 agccgcgtct tttgtcccgg ctgaggctcg tcttgaaccg atttatgcgg agactccggc
     5341 agcccccaag accgccatta attggaaaaa tctaactttc gttagttttt tggaaaatgc
     5401 ctcgaacgaa aacgggaaag tcggccagca agctagcagc ttggcggaga gggagagcgc
     5461 tgccggcgaa tcccagcctt cggtcgggga agctaaccaa gagcggccgc aacaacagac
     5521 acggtctcag cgccgccgcg gttccagagc atctcaggaa ggcggagaac aacctgagga
     5581 gtcaagcaca gcccttccga tgccggcgac gcctgccatt tctggttggc gaatcattgt
     5641 ggaaagtcct caggtcacta gtccccaatc cgtgaagtac gtcatgccga agaaacgtgc
     5701 cggagtgatc tgcgatgaaa actttgtacc cttacagagt tttcaggctt ccattacaga
     5761 tggccctcga gcgatgcgtt cgccgattgg tttttggcat attgttttct ttgcttggat
     5821 ttgtaatctg gctatgcatc tgggcttgtc cgacatggcc atcttccgat tcgctaagcg
     5881 ttcgtggtac gggctttatt ccgctttcgg gatgtttctg ggacactaca tcgcctggat
     5941 ttgcgcgggg atcatgggag ccgcggcagc tcggttgaca atgacgcccc ttatgcaact
     6001 tgattcggga caggttgctt accttatcct tggctgggca ggcgtggtgg ccgttgttat
     6061 cgccggttgg acaacatcca atcccacgct ctaccgtgcc ggtctcgcgc tacagattct
     6121 cacgcccaat tggccgcgtt gggtcgtaac ccttctggct gggacgatta ctactatcat
     6181 tgcctgttct ccgtttgtgt ttatctacct tctcgatttc gtcgggatct acggtatttt
     6241 gctcatgcct gtgggagcaa ttgtcgtggt tgagcattgg gtgtttccgc tactcggctg
     6301 gcgacgtttt tgggcaacct accgaggaca atggctcaac atccctgcga tattggcctg
     6361 gggtgttgga cttggccttg ccctagtttg ttggaagttc cagtggctcc acctattctt
     6421 tttggtcatt cccgtttggt ttgcaacagc gttgacatat ctgatcttgg ccggactggg
     6481 aggggccgcc aggaaaacgg agggcgaaac gtccccaacc acgccgtcaa gtgattccgc
     6541 tggccgggaa agcacacctg ctagcacatt tgctggagaa aagaaaaatc tcgatccagg
     6601 tacggatact ccaggccgta ggccgcttct catgtatgct tttggctttg cagccatggt
     6661 ttgcctactc gggagctacg cctttgcatg gttcgtattc gcggggtatt ccacagccga
     6721 agacgtgaaa ccaacactgt tatatctggc attggcgtac tttgtctctg gcgtgatcta
     6781 tctctcgctg agggaacgac gcaccgcaac cagcgcagcg taagccaatg tcagccatta
     6841 aagaggtttc tttcatgttg tcataggagg atcgcggaat gcggcggtca attgttgtgg
     6901 tctgttgcag tgttttcttg gctcttcact cggtgggtct gatgtcaggg gaggagctaa
     6961 gtccggcacg tgtgacctat cttagatgcg aatatcgtgt gaatcccctc gggataggga
     7021 caactcgccc tcgcctcagt tggatcatcg aggaccctag aactggggca gctcagacag
     7081 cttatcaaat cattgtggca tcaagctccg agattctcgc cgagaatcgc ggcgacctat
     7141 gggattcggg caaagttatt tcctcggaga tgaaccagat tgaatacgca ggcaaaccct
     7201 tgcaatctcg gatgaagtgc tactggaagg tccgcatttg ggacgccaat cagcaggcga
     7261 cgccgttcag tgaacctgcg atgtggtcga tggggcttct cgacccgcag gagatgaagg
     7321 cccgttggat tcaagcctcg caaccacttg tccaccctgc caaagggacg cagttagatt
     7381 ttggagattg cccctgggtt tggtaccccg aggagaacac cgacccccga gaaaaagccc
     7441 ctgcgggacc ccgctacttc cgaaaggtaa tcgaattacc ctcagagaag gctatcaaac
     7501 gggcacgact gctactgacg gccgacgatt cgtttgaatt attctgcaac ggtcaaccgg
     7561 cgggacgtgg tgcgggttac aacgctccgc aaatcgcaga tctgaccaat cgtttgcagc
     7621 ccggtcgaaa caccttggcg attgtcgcca caaacgcctc gccgtcaccg gccggcgtgt
     7681 gtggtcggct tcgtgttgag ttcgaatctg gtgaatccag cgaatggctc atcgactcaa
     7741 cgtggaaagc ctttccccgg gctgtggctg gctggaatca ggtcgatttc gatgattcgg
     7801 cctggccatt tgccgtcgtt gtaggcaaga tgggcgacaa accgtgggga gcaatttcgg
     7861 ctcaagggct cgacattttc ggctgtggat tgttccgcaa ggcatttcga atcgataagc
     7921 ccatccaaag cgctacactc tatatgagtg ccttgggagt tggggaaatc catatcaatg
     7981 gtcagctcgt taacgatgaa tacttcgctc ccggttggac cgattatcgc aagcgagtgt
     8041 attacaatac ttacgatgtg acgaatcttg tccgacaggg cgataacgca atcggcgcca
     8101 tcctcgcggc cggctggtat gctggggcga tcggctggaa aagtgaacga tttcactttg
     8161 gccgtgatcc gaaactatgg gtccaactgg aaattgtcac cagcgatggc gaacgcatga
     8221 ccattgtcag cgatcgcacc tggaaataca ccttcggtcc tcacgttgaa gctgaattcc
     8281 tggcaggcga aacctacgac gcccggctgg agattcctaa ctggtcactg cccggactcg
     8341 acgagagcca atggcaaccg gtacaggagg ccaacgacgt ccaggttctc ctcgaagcgc
     8401 agcgcggcgt tccagttata aggaccggtg agttgcctgc caaaagtgtc tccgaaccta
     8461 agccaggggt gttcgtcttt gatctcggtc agaatttcgc gggttttgcg cggttaaaag
     8521 tccgcggtcc caagggaacg gcagttcagc tacgatttgc agagatgctc aaccctgatg
     8581 gaacgattta cacgacaaac ctccgctcgg ctcgcgctac cgacacctac atcctcaaag
     8641 gagagggcga agaagtttgg gagccacggt ttacattcca cggtttccgt tatgttgaag
     8701 tcactgggta tcctgggcgg ccacctttgg atgcgatcac ggggattgcc attaactcgg
     8761 atatcccttt ggtcgggagc tttcgatgct caagcgaaat ggtcaaccag ctctacagta
     8821 acattgtgtg gacgcaacgg gcaaacttca tttctgtccc aacagattgc ccgcagcgag
     8881 atgaaagact gggatggacc ggcgacgccc aggtcttcat ccgtgctgcc acttacaacg
     8941 ctgatgtggc agccttcttt aacaagtggt tagttgattt ggaagacgcc cagggacctg
     9001 aaggcgattt tcccgatgtg gcgccgcgtg tcgtggccct tgggggtggt acagccgcct
     9061 gggcagacgc cggggtcatt tgtccttgga cgatttactt ggtttaccat gatcggcgcg
     9121 tgcttgagga acactttgat gcgatggagc gatggattgc gtactgtcag aaacacagcc
     9181 agaatctact tcgtcccgcg gccggttatg gtgattggct ttcgatcaag gccgatacgc
     9241 ccaaggatgt gctagcaacc gcattttttg cctacagtac gcacttgacg gcggagtgcg
     9301 ccagggcact cggtctggct gaaaaagccg cgtattacga caaattgttt gaagagattc
     9361 gtgccgcttt tcagaaggcc tacgttagcc cggatggccg cgtgaagggt gatacgcaga
     9421 cttgctacgt tctggccctc tatttcaatc tgttgccgga ggcgttgcgc ccgaaggcgg
     9481 ctcagtacct cgtggaagat atccgaaagc gcgacacgca tctttccaca ggcttcgtag
     9541 gtactgcata tttgatgccg gtgctctcag aaaccgggaa cacgccgttg gcttaccgcc
     9601 tgttattaaa tgacacgttt ccgtcctggg gattctcgat caggcacggg gcaacatcca
     9661 tttgggaacg ctgggacggt tggacaccgg aaaaaggatt tcaggaccct ggcatgaact
     9721 ccttcgctca ttattcgttt ggggctgtcg ggcgatggat gttccaatat gtcgccggta
     9781 tcgacacaga tggcccggga tttcgacggt tgattctgaa gccgcacgta gccgannnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttatg agttcagcgt cttgccgcca
     9961 gaccaaaagg actgagtcac atgttgaata agagaatcgg catgattggc gcggggcaaa
    10021 tggctctggc cttggcagca gggtttgtcc ggaagggtct cgttccgccg gagtacattc
    10081 tggcttccga ccctctggag aacgcccggc aacgattcca ggaagtgact ggagcacgag
    10141 tgacggataa caatcttgag gtcgtgcgaa cccaagatat cattatcctg gccatcaagc
    10201 cccaagtcat ttcaaaggtt tttgaggaac tacaaggccg gataagtccg gaaaaactgg
    10261 tgatttccat cgtggcaggc gtgactttgg agacccttca gcgggcgtta ggagaaaacg
    10321 cccggatcat ccgggcgatg ccgaatacac ccgcgctcgt aggatctggt attaccgcct
    10381 acgccgttgg aaagaacatt caaccggcag acgtgcaaac cgtcgaggca atcctcagtg
    10441 ctgttggcgt cgccgtccaa gtggaagagc gcctgatgaa cgccgtgacg gccgtttccg
    10501 ggtctggacc ggcctttgtg tatttgttta tcgaagcatt agtggatgca ggcgtccggc
    10561 atggtttacc tcgtgagttg gctcgccaat tggttgtcca tacggttcgt ggggccagcg
    10621 agatggtgat ccagtcgggg gagcatcctg ccctgttgcg tgaccgggtg acaagccccg
    10681 ggggaaccac cattgcgggg ctagcagcac tggagacaaa cggcttccgc ggggcagtgt
    10741 tcgccgcaat cgaggccgct gtgcaacggg ctgaagaatt gtctcgaggt tcgcgctagc
    10801 gatagtatta gaatgagttg tgcaaaaact gcgacgagcg ataatcacta caagaaaagg
    10861 ccattcgtcc tgtgaacgct gaaaggaggg tgttatgtct tccgtcgaat attcgcataa
    10921 cggacctcgg aatgtaatca tctgcctgat ttgcgctatc gctttggtct tcggcgcaac
    10981 tggttttggg caagaagtct gtcgtcagtc ggcgcgggcc attccgatcg tcgctgacgt
    11041 ggatgttgcc gtcatcgggg gaagtacagg tgcggtagcg gcggcagtca gtgcggcaaa
    11101 tgaaggcgcc cgcgtctttt tggccgcacc gcagccctac ctgggcgatg atatgacggc
    11161 aaccctccgt ttgtggctgg aacccgacga aaagccgaca acggacttgg cgagggcggt
    11221 gtattccgac ccattgcgtc cggctcccga agccaaccgt gcacgttacg gattccgata
    11281 tcagttggat gtccccagcg atccaaagca tcgagacacc agcccgccgt cgatgctcac
    11341 cgatgggaaa ttcatgcacg cccccaccga aagtgtgcaa tacaacagcg atgccacgat
    11401 tacggccgac ttggaaacac cgcaggtggt cgatcgcgta aatctcatct tcttctggcg
    11461 accatcgggt gaaccgggtg tggggtttaa agtcgccggt attgaggtga gcgttagtga
    11521 cagtgggaaa gactgggtat ccatcggcaa agccgatgtt acacctccgc aggctgccag
    11581 tggtgatcca ccgctttccg tcagggtcga tgttggccaa aaaaaggctc gctttgtgaa
    11641 agtcttcgtc cgcaaagcac ctgacgtgga acgaatgctc ctcggggaac tggagattct
    11701 cggcccagcc caagagccgg ttgaaaggga gcaagccaag taccttcccc cgcgtccgct
    11761 ccatgtcaaa caactcttgg acaaggtgct tttggatgcc aacgtccaat acctctacag
    11821 ttgttatccc acggatgtgg tacgcgatgc gtctggccgg gtttgcggcg ttgtcattgc
    11881 aaaccgcgcc ggtcgtcagg tcgtcttgtc caagactatt atcgacgcca ctgaccgggc
    11941 tacagtcgcg cgtctggcag gagcaaagtt ctctaaatac cccagtggga cgcatgtttt
    12001 cactcgggtg gttatcggcg gagaacccgt aaccggggaa ggattggaac atcgtgtcat
    12061 cgatccccca ttcgtaggtc aatttcccaa tcgtgcccgc acgtcgagcg gnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgtggatt ttggggggat gtgccgctgt
    12241 gtcgcgagag caggcagaaa agcttttgcg gccagttcac cttatggaat tgggcgagcg
    12301 attaggccga gaaatagctc gaattgcatc ttctacacct aaacctgaat cgcccgttgt
    12361 ggctgtgaac ccaatccagg ggaatcctat tgccggcgac gtgggcgagt ttctctacgg
    12421 ccttcgtcct gttaagaaat acccaacgat tccccaacct gagaccacga ttcccgtttt
    12481 gggtaaatat gacgtggtcg tcattggggg tggaacgggc ggtgccgccg ccggtattgg
    12541 cgctgctcgc caaggggcca aaaccttggt tgttgaatat ttgaccacac tcggaggcgt
    12601 cggtaccgct ggggctatct cgtcctatta ctggggaaat cgtaccggct tcacagccac
    12661 ggttgatgag ggacgagcca gttgggtcat tgagcaaaag gcggaatggt ggaggagcga
    12721 gatccttaag gccggtggtg aaatttggat gaattgccta gggtgcggag cttacaagga
    12781 caacgggcga gttaaaggtg ccattgtggt aacacccttc gggcgtggtg tggttttggc
    12841 tgatgtcgtt atcgactcca ccgggaacgc ggacatcgcc gcggcagccg gggccgaaac
    12901 catgtatgtc gatgataccg aacccgcgat gcaaggaacc ggcttaccgc cccgtgagtt
    12961 gggtgctcgt tacacgaaca ccgatttcac catcactgat gagactgatc tgatggacgt
    13021 ctggcacgtg tttatctatt caaagctcaa atacagcagc gcctttgacc agggccgcct
    13081 ggttgatact cgtgaacgcc ggcggatcgt tggtgaattc acaatgaccc ttctcgatca
    13141 agtgttgggg cgaacgtacc cggataccat ctgcgtggcc tacagtaact tcgatacgca
    13201 tggattcacc attgatccgt atctggaatt ggaacacccc gacagaaaag gtcttcgtgt
    13261 caatattccg tatcgttgtc tcattcctaa aggcttggag gggcttctcg tcaccggact
    13321 gggagtaagt gcccaccggg acgccatacc tttaatccgc atgcaagccg acatccaaaa
    13381 ccagggttat gcggcagggg tcgcggcggc gtggctagcc caacaggacc ttcccgtccg
    13441 ccaactggat gttcgcaagc ttcagcaaca cctggtggaa atggggaatt tgcctgaatc
    13501 cgtcctcaaa gaagaagact ccttcccgct tccagactcc gtggtcgcgg aagccgtcgc
    13561 tcggttgaaa aacaattacg acggcgctgc ggttgtcctc gcccactcag aaatcgccct
    13621 cccgaagctg cgcgaggctt acgccaaggc tcagaccgac gaagaacgag tcatttacgc
    13681 ccacgttctc gcggtcttgg gagataacac gggattggaa acgttaatgg cggaggttcg
    13741 caagttcccc gagtgggaca agggctggaa ctaccaaggc atggggcaat tcggtcgggc
    13801 gctcagccgt ttggacgagc taatcattgc cttaggtcgg acccgcgatc cccgggctgt
    13861 gccggtcatc ctggaaaagg ttcgcctgct aactcccgag tcagagttct cgcatttccg
    13921 tgccgtggcg cttgcgctgg aaatgatcgg cgaccggagt gccgcccgcc ggctagctga
    13981 attgttgacc atgcccggca tgtccggata tgtccatcga acagtgcagg acgcgattcg
    14041 ctttgaccaa tcaagtccag ggggcaccaa tgcggtgcaa acgcgacggg attccatccg
    14101 tgagctctcg ttagctcgcg cactttatcg ctgtggggat tacggcggta ttggggagca
    14161 aatcctgcgc gcttacactg aggatctccg tggccattta gcgcgtcatg cccaggccgt
    14221 tctcaataag ggccgttagt catccattta tccgtgtcga aaggaaggac gaatgacttg
    14281 ggtgtgacta tttgtctcag gtcaccggat tgagcaagcc tgtataacgc tggaagaaat
    14341 acctcgccgc ggcagacggc tcatagcgat acggtggcgg ataggatcga tagatcgccg
    14401 cgagaagctc gtcctctggg gcaatcactg cttgaatggg acgggcaaga agaatctgta
    14461 gttcttgcat atgccgggag cttggcggtt gggacacggc aaggataatc gtctcgccat
    14521 ctgtggcaat gggcaagaca caaagatgcc gggcgacgcc gccatcgacc aacttcaata
    14581 tatcctctgg tacttgaacg tcgtgcagtc tcacgaatcg acaatctgtg ctcgcagcta
    14641 gcatctgagc cacctcgacc tgtcgcgagt cagccatgaa ttcgatcaag gcggcgaggc
    14701 cctcggctgc cacatgcaac cgtacttggg cttgaatcgt ctttccggca actgtcacct
    14761 gccactcgct gtggttcacc aaattggcaa gcaaccttga gaggatcggc aagaaccagc
    14821 aggccggcac acgcacggtg gacaaaatgc gattctggat ccgataagcc acctcaagat
    14881 actcaggatg cggaagaagc aacatgtggg atgcccggta atggcgtgct tcagccacaa
    14941 atagattaag catcgcctcc gccgtcccgc aggtttcgtc aatgacatcc tgcacaagcg
    15001 tctgttgcgc caatgcttcc agccaccggg gggctaactc gatgttctga accaacgatg
    15061 gatcgcgagt gacaccaacg atactccgca ctaattcctc cgactggttc gaaagatagt
    15121 taagcagggc ggcattcgtc tcaattttgg ggttcgtttt caatgcatag caatagagaa
    15181 aattgaacca ttcatcgatc aacatcaccg ggtcgatggg aacatttcgt tcaccctccg
    15241 caagtcgaca cgccgcgaca taaagggcat aagcaatcgc caggtggcgc gcctccggat
    15301 gtctcacgat gatattgaag atccgcgcaa taattgcctc tcgaaccagc caaaggcgat
    15361 tttccagata gctgcgatat gcctgcggta acactgaaaa cagatccgcc cgaggaataa
    15421 cgacctcaag ttgccagcgt tttgaagatc ggatacagag tttcgggcga cgccggatat
    15481 cctcaaacaa ctccagtaac ttttctgtcg ccatttgccg cagataccca aaaatggctt
    15541 ctgcaggatg gccctgaggt ggcgatggtc gctcccacgt cgctagacgc ccggctactt
    15601 ccgcagtcgt catcggccgt gccaactcgg cgaggagcac tgttaccgcc tccggttccg
    15661 ccggtccgta gaacctttca atcgccgcgc ggagccgtcc ctccggtgcc agcatcactt
    15721 ggaagggtcg ccccaatgca aatcgcaatt cgtcctgcaa ctgtggaccg ggtggctccg
    15781 ccatggcaat cgcaaccacg tcgttggtca ccgacaaggg caaaacgtag tacgttcgcg
    15841 caatgtcgcg cggaatcgtg gccaagacgt ctgctggaat ctgaggcttt tccagttctg
    15901 taaacgggta actcaagagg ctggcccttt cccgcgcagc ctgaatcgcc gattcgtctt
    15961 tatcaaactc gataaccagt gccaggccat aaggggtggt acgacaagac gccttcagcc
    16021 gccgctcagt tccccgaacc accaccttga ttgtcccgta gaaattcgtc atccctatga
    16081 ggcgtgcagc aatcggtgcc aataattcgc caggcaggtc atttcgtgaa cagatgccgt
    16141 cttcgacacg aaagccgacc ttggtgacac catgataaga caccagggcg acccgggatg
    16201 cccccaaccg aatcccttcc cctacgatgg tctgaattgc ccgtgttacc tctggcgaat
    16261 ccgggtcgat caccacctcc gttgccgctt gtgattcctc gggctcaacc tggatgaatt
    16321 ccactgcccc tttctccgac tctttcccct cgccggtcga tggagactcg gcctcctggg
    16381 catctttttc ttctccgtac catttggcga tcgcttttag tagatgctct ttcacggcga
    16441 gtactgttcg tacgggacag tccagtgtga atcgcagtcg gtctgactgt tcgggaagca
    16501 gtggctcact cacggcaacc agcaaatgat cacttttacg ctcgagcggc aacaacccat
    16561 attctcgagc aacccgggca gggacagaat caagggcttc ttgtgacggc tgaaccttac
    16621 gcacatccac aaacggaaag cccagtcgcg tggccctcag ttccgtcagc tctaccaacc
    16681 gtgcgtcggc gtcctggttc tcatttccgg ctccacctga actgcgtcgc gcatcttcgt
    16741 tgaggctcat tgcgcacctc cgatcacatt cccggactca tcgacgcccg tcgctatttc
    16801 agaaaatgag actcacacgt tttcacggcc gagagccgtt cccattaacc tttctcgtgg
    16861 acaggcatct ttcgccgaca aatacgcagg caagtcctca caacgtaaga atagcgcttc
    16921 cacccagcac ctgtgcctta ttatagcgtt tccctaatca cacctgcaaa ttgctacaaa
    16981 tgagttctcc aggcccaata ggaagcgctt gttccaaaaa gaaccagaaa agacggacgg
    17041 ccgcgattcc aagattcctt atcgccgcgc ctacgataat taccaacgcg ttatccacga
    17101 gatgaaccgt ggttggtccg ccggaacccc aatcggctaa cgacttttag tcccttgacc
    17161 ggcgatacct atcggtccaa cttagagcct atcccataat cttccctgac ccgttcggca
    17221 ggagggcggt cgggggtgag cgtgctcgaa tcgctgctgt ttttctctat caattccgac
    17281 gttcgcttgc cctcacccca accctctccc aaagggagag ggggactttt tgggataggc
    17341 tggtcacgtc gtgaatgatc tactgttggg gaaaagccgg tgcctggtcg tcaggctccg
    17401 tgcgtcgatc tggcgatgcc gaaaccggga gcggcaccac cgagcgagcc gctcggaata
    17461 ctccacccac catgcgcccc agggagatgc ccccatcatc ctcagaggcg gcgctctctt
    17521 gaatcccaac cccggcatcc cccgcttttg ccgcctccgc gacggccgac tggtcgctcg
    17581 tagcctgttg cacagttgcc gtaccgggct caccttcgat cgtgctcgcg gggcctccgg
    17641 cccaatcctg attcgttgcc tccatttgac gggtctggtt ttccttggtc gcttggggct
    17701 tctctccggc cttctgacac ccaactccca cagcgtatac tatcgcgaaa acgagagtga
    17761 acacgaccgc tttttcaaaa cgcatgagcc attccctttt gtctaacagg cttccaccgt
    17821 caacccctcg cacccacata acaatggtcc atcatacgcc acagcgttgg ctaagaaaag
    17881 gcttggcatg acacacacgg cacttaactg cagtgttctc aatgagccgt gcctttcgcc
    17941 agtggacgct gcatcaatac cgataagcca gacacatcat ggcgccgcaa cgcagaataa
    18001 aagttgaact gcttgagttc caatatttcg aacaggcgtc cgagttccca gcggatactg
    18061 tcctccgaaa caggtggaat gccgaccagg ctttcgccac cgagatatgt gatacagaga
    18121 tagtaagagc cgggctttgt cacacgccag aggaggtcaa gcagcctcga caactcccat
    18181 tggcgtatgt actgatagaa cgcgcaatca tagatgaaat caaaggtttg ggtgcggtat
    18241 gcgaaatgaa agacatcgtc gaggacaaag caaacattga cgcccgcccg ttgggccctt
    18301 gttcgggcac gttcaatcgc cgtcggagcg aaatcaacgg ccgtcacatc gaggcgccgt
    18361 tgggccatga agacagcgtc ggcgcccgtc ccgcaaccca gctccagcac acttgctttc
    18421 tgtaatagcc ctttatccag gaaagcaacc aactctgggg acggacaccc catttcccat
    18481 ggcggcagcc cttcgcggta ggccaaatcc cagtcggccg ccgttggtat tccccggcga
    18541 gtcacgtgac gattttcagg gctatcatcg ctcattgttt tgctcgcccg tgttgactcc
    18601 gagtgatgca aaatcccacc gacaatccga tatttgccgt cgatgcgaag caatcgcccc
    18661 taagatacca caaacaaaat ctggcttcta tgccaagatg ttacttacgt acccagcgcc
    18721 gtgtcatgtt cacagccaag atgccccttg ggtcccgtta tatcgttacg gcctttaccc
    18781 tataaatgga ttggctaata gactccttca aacttcagcg agctaaaaaa tgggaaatgc
    18841 aaccaatttc ccacgcttgc cctctgtctc cgatggggag tcaggacctc ttcaggtccc
    18901 gaccatccca gtgtcggcga agaaccatta cagaatccca ctcggcgaca aatcgatccc
    18961 ctccaaacgg tgcagtagct acactcctcg tgggtataaa ccaacctttc cccgatcagc
    19021 taccggtttc cccatatcgc gttctatccg atataacccc tgatggggga taaattaggc
    19081 cattgtgttc ttttcccagc gagcaccaat tgacaacgcc gtgcggcgta aatacaaggg
    19141 tgagataggg cttaggaccc aatttcctag accttttcaa cagggtttta ggtatgccca
    19201 aaggtgcaag aacggcgaag gcgtttcgct taccggccaa accgaccgtg gccaaattgc
    19261 aacaagcgct ggccgaccta gaccagcgtc ttctggagct gtgcaatctc cgtgccgaaa
    19321 tgagtgtcca actttcacag cttcgccagg aagaaggcca acctgatttt cagctttccg
    19381 atgaagacag gcaactggcc gcggtggtcg aacagaacaa gggcccctta ccgcccgctg
    19441 ccatcaaagc tgtctttcgg gagcttatta gtgcgtgtcg ggccatgatt aagccgttgc
    19501 gcgtggcata tttgggcccc caatacagtt atagccattt ggccgctatc caccggtttg
    19561 gtcagcaagt ggactacgtc gctgtgggga gcatcgccgc cgttttcgaa gaggtcacgc
    19621 gtgggcattg ccagtttggg ctggttccct tggaaaactc caccgatggt cgcattgctg
    19681 atacgctgga catgtttatc cgcacaccca tccgcatttg cggccaagtt gagctggaaa
    19741 tccatcactc cttgctttcg aagtcctcga ggtccgaaat tcgagaggtc tacagcaaac
    19801 cgcaggcgct atctcaatgt cgcaattggt tggcaaagca cctgccaggg gcacggctga
    19861 tcgaagtcac tagcacctcc accgcggccc aactcgccgc cgagaaaccg ggggccgcag
    19921 cgattgccag tatccaggca gggatacact atgggctaaa cacactggca gaaaacattg
    19981 aggacaatcc cggcaatatg acgcgttttg ccgtgatcgg taacgtaatt tgcccgcgga
    20041 cgggtcacga caagacggcc ctcatgtttc aggtggaaca ccgacccggg gcattagccg
    20101 aggccatgaa tatcttcaag cggaatcgct tgaatctcac atggattgag tcgtttcctg
    20161 tgcccggttg tcgcggggcg tactactttt tcgcggagct ggagggacat gagtctgaac
    20221 cgcgggttcg tcgggcactg gcaagtctaa gcaagaagac ccaacggctt gagttactcg
    20281 gctcttaccc aattcccaca ccggttagca cctgattgat gcaacttgcg gtccttcttg
    20341 acaacaagag tcttatgcga gttgacggct ccgctagtag ggtacctcgt tggtttcgtc
    20401 agtctgtttg ggccattggg aaacccggca caccttatgg gacgtggtcc attgaaaagt
    20461 gaaacacagg cgaaagaatc agcaccacac cttttgcgcg tcatttctgg tggacagacg
    20521 ggagtcgatc gagcagcgct cgatgcggct atccagctca atattccgca tggtgggtgg
    20581 tgccccttgg gacgactggc cgaggatggg aaaatccccg actgctatca gttacgagaa
    20641 acccattccg ccgattacgc agtccgaacg gaatggaacg tccgggacag cgacgccacg
    20701 ctgatcctca ctaaaggcga acttagcggc ggcacgcgac tgacagccat cttcgccttg
    20761 cggtattcta aacccctgcg agttgtgaat cttgaagaca atcttccgcc gaaaagcgtt
    20821 gtcgactggc tactctccca caacgtgcga acactcaacg tggctggccc ccgggaaagt
    20881 acgtgtcctg aaatctacca aatggcaaag gcatatcttt gcgatttgct ccaggagtgg
    20941 caacgccggt gttcccaggc ttaattcgtg cgcggattag cacggatagt tgctgaattt
    21001 ccccaactct cgctgccgct actgattctc tttcacgcgg aaggcatagt ttggattgcg
    21061 gatatcaaaa tccagatcgg tgaatccatt attcaccttg atgttaaggt aagtgtactc
    21121 ttcgatgagc tggggtgcac catcgggctc ggtaggccag tcgtaggact cgtacctgac
    21181 cggcaggttg tgctcggtat caacgtagat tcgggcaaga tgaaatcgga aattgcggcg
    21241 tggcaccggg tgaatgacct ggatacacgt gcacgttcgg tcgttgatct tcgctccttc
    21301 aaagaacttg acctcgcact cgccatattt catgtcctgc tcgccaacct caatcagccg
    21361 tcgaacgagg ttgaggatcc cgatttcggt aatcggatac cgattgttct gcatcgcaat
    21421 aggaccagtg ggatccagcg aaacggttcc gatgagtttg tttttgatcc cggcgggatg
    21481 cgcccacatc ttgccatcgt tttgaccttc aacgtagata acctcttgcc ccttgacgga
    21541 agccggctca aggaagtgca tgtaaacact gaagggttta tgccgaatct tcacaaaggc
    21601 atactccgga tcgttcagtt tcccgttgac ccgctcacgc ttgaccatga tcgcagagta
    21661 gtcttgaatc ttttgcagat cctgcagccc ttgataggcc caacggatcg ccggcatcaa
    21721 gggatgctcg acgaccgggt tggtttgagg taccacacgg tcggcgactc tcaactgcga
    21781 gtcactctgg ccactttgag taggcgtcat tggcgattga ccaaaagtgg tccggccaga
    21841 agcagtccag agaaggctga gacagctgac gagactgtaa accatgacgg ctgacacaat
    21901 tcgtcgagaa aaggatgaaa tacgttgtac catcgttatt cccctaaata acacaaaaaa
    21961 caagttattt ccttgacgcc actgcgggct tgcctaacac aatctccgtc cgaaattccc
    22021 tgccgcgacc aacctctcaa ggacacctcc gagtaagagg tatctatcta ggtgccatcc
    22081 atctggcgct acaacccgct attattgccg tatcgctgat taacatccgg aacattaaaa
    22141 tgatcgttct tagcgaaacg cattccgcag gaggatgcag ccccctaacc ggcattaatt
    22201 cctccgtcag caatcacgac gtattcactc taaattacag gagaactcat ggcggacaga
    22261 acccaccccc gcgtatggac gatcgtctca gcaatgttcg accaaaattg ctacgtcgtg
    22321 gcaaatgacg attgccaaga gtgcgtcatt atcgacccgg gtttggagcc gggaaagatc
    22381 attcgttcga tatccgctca aggtttaata ccccgagcca ttctcattac gcatggccat
    22441 gccgatcaca ttgcggggat cgaaggggtg tggcgccgct ggccagagat tgaaatcgta
    22501 gtgggagaac aggatgccga aaaactgacc aacccccggc tcaacctctc ggccgacttc
    22561 ggtttcccgc tgacgacccc acccccgacg aagctcgtcc gcgatggtga tcagttaaca
    22621 atcgctggtc tcgagatcct cgtccgtgag gtccccgggc actccgctgg acacgtggtg
    22681 tacgtcgtca atcaagccac gccaccgtta gccttcgttg gagacgtcat ctttgcggga
    22741 agcgtcgggc ggaccgactt cccgggcggg agcatggaac agttggccga gggcatccgg
    22801 agcaaaatct actcgctgcc ggaagaaaca gaactttggc ccggccacgg ggaacccacg
    22861 acggtcggtc gggaacgacg aactaaccca ttcgttcgcg gctaagcttc ggaacgcact
    22921 atttggggga ctaatccctt acaatgtgat tgcgaggtgt gggtcgcccc cgcttcccgg
    22981 caaagtctgt aagacgtccc caaaatgggc taactgacgc gacaacccac attgaccgtt
    23041 acttggtatt gcgtaatctt cccatccgaa atggcccctc gcaactcgac cacttcaaac
    23101 caggagaggt ttttctccgt ttcggcaact ttgctgacgg ccttagctgc ggcatcactg
    23161 aacgactcac tcgaagtacc taccacttga atttttttgt acacagcgtc tgccataagt
    23221 ttcacccttt cgtggcacct cgcctgatat ccaacttact gctaactaat acgctcacgg
    23281 aaatacagcg actcgacggt cgcgcgctac tttgataaga taaccggttc acccgcatag
    23341 aaaaaggatc gtgaatcgaa aagggttgtc tcgatgagtg gggaacaacg tcgtgtggtg
    23401 atcaccggcc tgggggtcat ctgcccaatt gggacgggtg caactgagat ttggcaagct
    23461 gtcacgaata ggcaaagcgg ggtctcctcc ccgtcttgct tgccaacgga gacattaccg
    23521 atcaaagcgg ctggagaagc cagacaattt acgggaaata ttaaagattt tggggagatg
    23581 gacaaagagt cccaaaaaaa tctccgcaaa tcgttgaaag tcatgtgccg ggagtgccag
    23641 atgggtgtgg cagccgctca actggccctc aacgatgcgt ccctcaccca ggggtcattc
    23701 gatcccgagc gggtcgggac cagcttcggc agcgactaca tggttagctc ggccgaggag
    23761 tttacggaag ggattcgggc ctgcctcgac gggaacgggg tgtttgagtt ccaccgctgg
    23821 gccagcgacg gcatgccccg tttatttcct ctctggcttc ttaagtactt gccaaatatg
    23881 ccggcaagcc atgtggccat ctataacgac ctccgaggcc cgagtaactc catcaccatg
    23941 cgtgaggcca gtccggctat ttgtgttcgt gatgcaatgc acgccatcca aactgaccga
    24001 gaggatgcgg ccgttgtcgg ggtgacggga actcgattga gccttatgaa ggccatgcac
    24061 gcgattcagc aagaggaagt tgccatttac gatggggaac cgcaacgggc gtgtcggcct
    24121 tttgatcttc accgcagcgg gatggtaccg ggggaagggg ctgccgcaat catcctcgag
    24181 gaactgaaga cggcgacccg tcgcggggcc tccatttacg gcgaggtcct cgccgcctcc
    24241 tccgctgcgg ttttggagag gaaatgtgtc gcccggcggc gagaagccat gcgtcaggct
    24301 atgctcaacg ccattcacga ggcgggattg aagcccacag atatcggatt cgtgatggcc
    24361 catggattag gtactcaaac ttgcgatgtc gaagaggccc aagcgctaag tgacgtcttc
    24421 ggctcgcgcg gtgtgcccat cattgctgcg aaaagctatt tcggcaatct tggggcagcc
    24481 agtggccttg tggagatgat cctcgggatc ctcgcgctgc ggcatcggca gttgtttgcc
    24541 acgctcaatt acgaaacccc ggaccccgcg tgtcccgtgg atgttgtcac ggagccccgg
    24601 ccaatcgaga aaatggtgtt catgaatgtg aatgtcactc cccaaggcca agcctctgcg
    24661 gccatcatcg cggcgtatga tggtacataa ccctaagtga acgaagacaa ggtttttgac
    24721 tgccggtctc aagtgggtag gttgagcttt gtcgagccga ggagactgcg cttgggttga
    24781 caggaaacat caccttgacc atgagctcgt cggatcaatc gatcatcatc cgcggggcac
    24841 gcgtccacaa tttgaagggg atcaatctag acatacccaa agacaggatc gttgtcatct
    24901 gcggtcccag tggaagcggc aagacgagcc ttgcctttga cacattattc gtcgaagggc
    24961 aacgtcggta tatcgaaagt ttctcgcccc gcatccgtca gcgattggat gacctaccgc
    25021 cgtcagaagt tgatcacata tcgggccttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn ncatccaaag cagccacttt aacctccgtt ttggcagatc tccgtgcttc
    25201 gggctgggcg cgtgtcatcg ttggaaatcg catcgtcaat ctaacggaat cttctctcgc
    25261 agaagttgaa aagcagctcg tgggtggcga gtcccagaga tcagaaggtt ttgacaagtt
    25321 aaacgaccat cggtccgttt acgtggttgc cgatcgtttg actattcagg agaaaaaggt
    25381 accgggtcgt ttccgcgaga cgctcgatcg attggagaac gagggcaagg atgttgtgac
    25441 gctctttatt cagggaaatg aaccgcttgt caccagcagc acagcgggtg cacaagccgt
    25501 aacgatgatc atcgaagggg agcggtggat acgatacgat tttccgttgg cgctatgttg
    25561 tgcgacatgc cacattgact acgaactgcc gcatcccgac ctattcaact tcaatacggc
    25621 agcgggggcc tgcgagacgt gtcaggggtt gggaactgtt gattcgttag aggcgagcca
    25681 gatcttccca gaccgccacc tcagccttaa ggatggggca gctgcaccat ttcggtcggc
    25741 gagcttttcg cgatttcatc tcaggcttct ggaattcgcc aagaggatcc gaattcccat
    25801 tgaccgcccc gttggtgact tgccacaaga gtacatcgac tggctgttgc acggctcgcc
    25861 agacgacagt tcgtcgtgca tcgaacagat ctttcgtgag cttgaacgtt accgctacaa
    25921 gcctcagatc aaaaccttct tgagccgttg gcgagttcca cgaacctgtc ccaattgtca
    25981 aggaagccgc ttacgtatcc agtccctggc cgtgagactg gtcccgaaaa cggcgatgga
    26041 gaactttttc ggcaagggaa tgacgccacc ctcaccgctc gaccagacgg aaaaacctct
    26101 gaacattgcg gagttctgcg aaaagtcaat tggcgacgcc cgtgcgttct tgaagacggt
    26161 ggtgcctaat gggcttaatc ctggagcgcg gctggccgtg aacgaagttt tgcggcggct
    26221 ccagatgctt gaatcacttg gactgggtta tttgacgttg agccgtccgg ccgacacgct
    26281 gagccaaggc gagttccgga gggtcctgtt gagcgccgct ctgggttcca gtctagtcaa
    26341 tgttctttac attctcgacg aaccttcgct gggacttcat cccaacgaca tccaccggct
    26401 acgcgatgtg attacgctat tacgtgaccg cggcaatacg gtggtcgttg tggaacacga
    26461 tgaagccttg cttaagtgcg cagattgggt tatcgaatta ggtccaggtg ctggtgaaag
    26521 aggtggcgag gttgtcttcc aggggacact cgaggatttg caaaagtcgg agaccctcac
    26581 cggagagtat ctctcggcga gacgacaaat caagatcgat agaaccagac gcacacctgc
    26641 cggctggatc aaactggttg gggccacggg acacaatctg aagcacgtca ccgcagaatt
    26701 cccgctcaat gttctttgtg tcgtaacggg tgtcagtgga gcaggaaaga gcagcctgat
    26761 tgaagaaacc ctttatcccg ccctgtgcca acgactgttt ggaacaagtt ctcccgccct
    26821 cccctttgag gaccttatag gagaacgaca gatctcggac gttattctcg tggatcaaag
    26881 cccgataagc aaaacatctc gttccaatcc cgcgacaatc ctcaaggtct tcgatgaaat
    26941 aagggccctg tttgcccaaa caagcgaggc caagacacga gggctgacgc cagggcattt
    27001 cagcttcaac gcgggtgatg gccggtgcgg gagatgcctc ggagagggac gccttgagat
    27061 cgatatggag ttcttgccca acatggagat cacttgcccc gattgccacg gtcgccgcta
    27121 tcgaccggat gtgctttcgg tcctttaccg cggcaaaagc atcgccgatg ttctcgactt
    27181 gaccattcgc gaggcctatt tcttttttcg gggccacgct acgatccagt ccaaactgcg
    27241 tcggctaatg gatgttgggc tggaatatct tcggctgggt caggccacgc ggaccctatc
    27301 cggtggagaa tcccaacggc ttcgcctggc gtcctacttt acacgctccc acaaaaaggc
    27361 aacccttttc attcttaacg aaccgacaac aggtcttcat cctcacgata tcgtgcagct
    27421 cctccgttgc ttcgaatcgc ttcttgccta cgggcattcg ctgatcgtca tcgagcataa
    27481 cctgcacgtc atggttgcgg cggactacat catcgacctt ggaccaggtc ccgccgagca
    27541 gggtggaagt atcgtcgccc agggaccacc ggaagaaatc gcacgcactc ctgaatctgt
    27601 aaccggtcgt tatctgctag atcttcttcc tcagatgagc tagctgggta tgattcacaa
    27661 tagggagcgc ccactttaag cccaacgctg gaggagaggg cggaaagcca aacgacgaga
    27721 caatctttta tgtcacagct tcacttgaat cgttatattc gtcaggttca atgcgcgaag
    27781 ttcggtgagg agtcgcagca acgcctgcaa acaagccggg tgtttatctg tggttgcggt
    27841 gcgctcggaa cgacgttagc caaccacctc gtccgtgccg gggtgggatt cgtgagactc
    27901 gttgatcgcg atcttgttaa tctccacaac ttgcatcgcc aggttctttt tgatgaggct
    27961 gacgctgctc aaagcttgcc caaggcgatc gcggctgtgg aacatctgaa gcgtatcaac
    28021 tcggaagtct cgctggaagc tatcgtagct gatgtgaggc cgaacaatat cgaccagttt
    28081 gccttcgatg tggaccttat tcttgacgcc accgacaatt ttgagacacg gtttttactc
    28141 aatgactatt cactcaaaca caaaattccg tggatttttg cggcctgtct gagcactcaa
    28201 ggacaggttt acccaatcat tccgggcagg acaccatgcc tgcggtgctt ggtccctgaa
    28261 ctaccgctgc cgggttcggt gccaacatgc gagatggcgg gcatactcgg accggttgtc
    28321 ggtgtcgtgg catccatcca ggccatggag gcaatgaaga tattagccaa gaaattcgat
    28381 tccgtggtgc ctgacctgat tgccattgat ctttggccgt tccgcatcca gcaaatcaag
    28441 gtccagtcac tgacaacgca agaatgtcct gcgtgtcact tggggcagta tgattacctt
    28501 tccacggtag gattgtctct gacaaccgaa ttgtgtggcc aaaaaagtgt gcagatcaca
    28561 ccaccttgcc cggccaagtg tgacctggat cacctaagtc aacaatggag tacactagga
    28621 acggtggaaa ggtccccgtt ctttctgcgg ttacggtcgg ctgggtgggt cctgacatta
    28681 ttccccgacg gtcgcgccat tattgacggc atcaacgatg ttgcccttgc acgatcgatt
    28741 tatgcgcaat tcgtcgggat gtgatccagc gacgggaacc tttccttcct atctcaaatg
    28801 gaacgtcggt agcactttta tcggatctgg ccgggccact ctttcagggg aacaatcttc
    28861 ggagcctccg ctttggcact ggggttgaat tgaaacgcta cggcgatcgt catcccgttt
    28921 tggggcaccg gcgtttgaac cgctaatcgg gtgatgccgg ggttctgtcg ctgaggccgg
    28981 gaagggttgg ccgaaagggt ctgaaacacc gccttctccg ggctgagcaa gaagacctta
    29041 acggtctttc cccccaacgc caactcggcc actttgccac gaacttgaac cttcgcctcg
    29101 gtatgaatct gccactgcac cgtttgaccc gcgtgtccca taatttcatc ttggacaacg
    29161 acccagtcct tgcccaagat agctatccca cgttgcacag aatcctttgc ggaagccgtt
    29221 tcattgcccg ggttgtaggc agccgttagg tcaactacgg catgttttcg gacaccagta
    29281 tcataacacg caagcacagg ggctttcgct cggggatttt gattttggcc gtcaaagagg
    29341 agcgtatttt ggccttcggt gcccaaacga tagtacgtcc atcgttgcga tccgaaataa
    29401 ccgggtaagt tgtaatcatc aggcccaagg tccagaaccc aacgttcgcc aaggccatcc
    29461 aacacaaaac ttcccagttc caaatggcta tgattgaccg agttatcccc gcctttgaac
    29521 cctacataaa tggcctgatc agaccagtca gtccgcatca tgaccacatc tgagttacga
    29581 aaccagcggg ttgtcggctc cttgtccagg ttcccgacgg gtttgaaata ccaccacaaa
    29641 tggagtggcg actgcgggct tcccacgtag tacagttcat gccaggcata gagcggcttg
    29701 tgaaacttcg ctgccaacca aagcataatc ggagcggatc ctacgcggtc cccgccgtct
    29761 gcaaagttga aggcccgcct cgtcggtccg atgacgtgga tgcgaaaatc cccagcgcgg
    29821 tcaaatcccg gttggttcga caaaccaaaa tcggtcccca gggccgtctc caaggccgcc
    29881 aacatataga ccgtgtaatg agtggcgtaa ctccaatatc ccggcccttc tgcccaagct
    29941 ccatccggtg cataattcgc catggcgtga ggcagcgact gcacagcctg ttcaacaatg
    30001 tactgagcta actgaggttc ctcgtcagca atggcgagtg caccgattgt cataccacca
    30061 ttgcatacct ggttccaatt ccaaggggtc ttggtccacc acgtaccttt ccgatacacc
    30121 ttttccccct ctttgaggcc tttttccacg atggcctcgc gaatcttttg ccgctgctcc
    30181 ggcgtcatga ccgcgtacag ccagtcatag ccaatcgcca aggcgtgggt catttcggcc
    30241 gtgtcgagga aatgtgaggg attccagtcg ggaaaatcag ccgccgtcaa catttcccgg
    30301 atagcacgat cggcaaatct tttgtcgccg gtcaatcgat acgacgtccc caagaggtac
    30361 attcgactca aacaacgacg actttgatcg agaagccgag gaccgatgag gcgatgccga
    30421 ataggctctt gttttaaggt gctttcggct tctcgctgga tagattgaaa aatctctcgg
    30481 gccattggat cgctcgaaag caacttcttc acacgttctt catccgtgcg aaggacgata
    30541 agccgtgggt gttcccttcg caaagtagtg agaacgttct cggcctgcgt ttttgcgatc
    30601 gaaagcaaga caaagaggac cgtcgcgacg gtggcaatcg agcgtaagac ccttaatggc
    30661 accactggcg ggcaaaccat ggttcactcc ttttctttac cgatcgtaca aagcttccag
    30721 gtgtcacgtt ccccttggaa caaccaaaaa gagaatgatc gagggcatca cgggcgcacg
    30781 tttggcggga ctacccaagt ttgcgtacga ggaacaacca tgcgcttagg ttgtgaactg
    30841 ttccgggtgc ctctaacatc acatgaacat tacatattct ttgcaatcgc tcgttagtgc
    30901 atatctcgtg aacagagtat agaatttgtc ggaaagcgtc aagcaattcg accacgataa
    30961 ttcatcttat tttctccatc caacgcctac ggaacagttt gcctaatcca tacccttgac
    31021 aaaggcgttg aggttgttag gctattcaaa ctaggcggaa agaaaagact tcttcgtcgc
    31081 accgcttttg aacagggcag cggtgagacg aagacggcct aacagcctgg tgtgttttgg
    31141 ccccgcaagc cgtcccgggg tcagacgagt gtcagaggca aagatgacgg catgtttcgg
    31201 agtgaatgtt ctcaaacgag gaggaacaac caatgaaccg tctgagcatc ttgagtctag
    31261 cggctgtgag tcttgtgatt gtcgctgcac tcgcagcaag tgctgggccg tgcgatcagc
    31321 catgtgcccc accggcttgc caacccgcct gcgagcagcc ggcatgcgaa ccggcgtgcg
    31381 aacctgcatg cgttcgcgtg ggattagtag cccgcctccg tgcctggtgt gaagctcgtc
    31441 gtgctgcagc agcctgctgc aagcctgaag catgtgagcc cgaagcgtgc aaacccgctt
    31501 gcgaaccggc atgtgagccg acatgcgaaa agcccgcccg ttgctgtggc ctgttggccc
    31561 gtattcgtgc tcgccgcgca gcggttgaat gctgcgaggt agccccggcg gccgagcaac
    31621 cggcacccgc cgcttcccca gccgaggccg cccctgcggc tccgcaaccg gcgccagctc
    31681 cggcaccagc cacctgaaat ggcagaggga aaaggcttcg cttaagtttc gggatttctg
    31741 aagcgaacga aagcccaagt tactataagt gcccagtgtg ccctgttcgc tgggacctga
    31801 cagctacagt gaaccagtcg cgcaccgagc cgatcccgat cagccggggt cggcttttta
    31861 ttgcgctagc cttccggccc ttccaggttt gtttgcctaa tgccacaatg aaggggagtg
    31921 acagacccgt gccaaggacg gggagggaaa acctcggaaa ggcccatcgg ccgacagaat
    31981 ctattggagt ccagggcaat tcctgctcgg aggcaagccc atgaaatgtc cctactgccg
    32041 cgaagacgac gacagagtta ccgatagccg aattgccgaa aatggatatg ccattcgacg
    32101 acggcgacag tgcaatcggt gtgggaagcg gttcaccacg tacgaacgcg ttgaacgaag
    32161 aaggttaaag gtcgtcaaaa aggacgggtc gcgcgtgccg tacgatagga acaagatcaa
    32221 gaccggcctt gagaaggcgt gttggaaaag gcccattagc gacgaacaga tcgagtcgat
    32281 tgtgacggcc atcgaggacg atatcgagaa gaacttcgaa aacgaagtgg aaagccggta
    32341 cattggcgag ctggcgatga aatatctgcg ggaattggac caggtcgctt acgtccgttt
    32401 cgccagcgtg tatcggcagt tcgaggatgt ccacgacttc atggaagagg tccgccccat
    32461 gttagaccag gcaagaagtg ggtgatcgct cgttttgaag tccacaaacg atacccttgt
    32521 gcacgaaacc aatgccgacc gcttggctgt ctgccaacgg catcttgaat ctatttcagt
    32581 acccgtccgt ctcgcgcttc tagggaagcg tttttgccgg aacctggttt tcaatgtgcg
    32641 gctccaactg ctgcgccagc ggaagtgatt cgcccttggg cggaccttct ttccgtcgcc
    32701 attggcggct catcttgtgt aataggacga tgacacacgg caccaaaatc gtccggatga
    32761 caagagtatc caagaggact cccatggcca acgcgaatcc caattgatgc atggttcgta
    32821 acgaaccgct gatcatagcg atgaacgtac ctgccatgat aattccacaa ctagtgatga
    32881 tcccacctgt cttgcgaagc gccgagcgaa gaccttcctc gaaaccaaat cgcgcttgtt
    32941 cttcgaccac ccgcgttacc aagtaaatgt tgtagtcttc tcccacagca accagaatga
    33001 caaataggaa ggtcggcaac ttccaatcca agccgtgata agtcgcgcca tgaagccatg
    33061 cgaacaatag ctcggtgaca cccaatgtca cgtaataacc ccaaatcacc gacagaacca
    33121 agaagatgga aagcactggt tttcgaagga tcagaatcaa aacaaatagc acggcaatgg
    33181 gaacaagctg ttggattaac ttctgatcac tcaacgtgac tgcccgcaaa tcccgaatgc
    33241 ccggtgtcgt acccgtgaaa tagaaggccg ccccttgcca agacggattt ttcgcagccc
    33301 actcattcaa gtaattttca agcgcatcta acagtcgaat gctttcgatc gaaaaaggat
    33361 cataaggaaa aacgacgtct aggcgggcaa ctttgccttg gtagggtccg gtctgagata
    33421 catacatcgt cttggcacga ccgctgatga caccacgccg tagtgagccg aatagcccgg
    33481 atcgcccgct tttctcacct aacggatcga ccaagctccg aaccgtctga atcggttcga
    33541 tccgatgacc ggccgagtcg gtgtattcca tcgcataaat cgtgctgacc aactccggca
    33601 accggcgata acggtccggg ccttcattga aattagcgtc cggatgatag acgaggaccg
    33661 tcaccgggcc cgtctcacct gccggaaaat attcccgaag aaggttagtc cccttgacac
    33721 attcacggtc cggcccaagt tcacttaata ggtcataagt aagatccacc tcccaggctt
    33781 gccaaacgaa aggggccatc acgagaagcg atcccgcgag gataagcccc ggtgctttca
    33841 acacgccgcc cgcaagccta ttccataaac cctgggaata attgccgagg ataccccttt
    33901 tccccaagct aatatcatcc gtgtcgtcac gcggggagag atcaacccgg aatggccaaa
    33961 agattttccg gcccaagaca caaaggatag ctggcgctaa agttagacaa gcagccaggg
    34021 cgacaattaa ggctacagca atcgcaggac cgccgttgcg aaatttccca aagtcggaaa
    34081 agatcatcat gccaagcccg aggatcgtcg tccaggcgct ggcaaatagt gcggcgctaa
    34141 cgccatcaag ggccttggcc gccgcctgga tcgtccccag tcccttactc aactcctctt
    34201 tgtagcgaga aataaggaac agacagtaat ctgttccgct cccgaacagt acaaccacaa
    34261 caaaaatctt tgtcgttttg aaaactgcaa actcaaaggc gtttgtcttc agactgatgc
    34321 ccgcaatgat ggcgagcagg tcaaaggccg tgaacaccgc agcagcgata gcgagtaagg
    34381 gaacgaccgc gagaagggga gcgcgataga ccaccagcaa gataatgatg atcaagccaa
    34441 tggtggccac ctctgtgttt cggatgcttt cttcggcagc gatgagcatt tctgtcccca
    34501 ccgcccctga tcccgtgact ccgagttcga gtcccggtgg tttctggggt gaccgcctta
    34561 gttcatccag cttctcatag atggccaaca aaggcttcat attctcgaca gacatgatct
    34621 cacccttgag caacaagatg atgagagctg cctgtccgtg atcagaaatg gggctgagga
    34681 gtttctttcc aacgacctca gtgcggggac caatgatatt cacaacgggc gattcctcgc
    34741 cttccttggg ctggtaggca ttcgtaatga cctccgagaa atcgaggtcc tgctc
//
LOCUS       DAOSUT010000019        34576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_872_length_34576_cov_8.968946, whole genome shotgun sequence.
ACCESSION   DAOSUT010000019 DAOSUT010000000
VERSION     DAOSUT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 34576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34576
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_34576_cov_8.968946"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(784..866)
                     /locus_tag="PLD05_02950"
     tRNA            complement(784..866)
                     /locus_tag="PLD05_02950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Ser,seq:cga)
     gene            1428..2642
                     /locus_tag="PLD05_02955"
     CDS             1428..2642
                     /locus_tag="PLD05_02955"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOP76418.1"
                     /translation="MAKCVLAYSGGLDTSVILSWLVEQGHEVHAVYVDLGQPCEDRET
                     ILAKARQIGAKTAQIIDAREELCRDFAFPVLQWQARYEDLYLMGTSIARPLISKVCLQ
                     VARQHGCDLFVHGATGKGNDQCRFQLAAEALQPGIRVLAPWRLAEFRERFPGRKEMIA
                     YCEQRGIPVKATASKPYSSDENCLHISYEAGRLEDLTVNGVELIEFGMTVPPQKAPDQ
                     SEKVTIAFESGIPVSVNGEKLSPYGIVNCLNRIGGRHGIGLVDMVENRFVGMKSRGVY
                     EAPGMTLLYFAHRQVEQITLDRDVMHLRDRLAPEVAELVYYGFWYSAKMDAFLAMIRE
                     IQKPVTGEITLVLYKGNMIVDRRSSPNSLYDEGIATMEGGGDYNQSDAEGFLRIQGLP
                     LRVQARIRPRSF"
     gene            complement(2756..3562)
                     /gene="trpA"
                     /locus_tag="PLD05_02960"
     CDS             complement(2756..3562)
                     /gene="trpA"
                     /locus_tag="PLD05_02960"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOP76419.1"
                     /translation="MSRISEVFKALRKEGKRAFMPFLTAGDPNLDFTVRLMHTLADEG
                     CSLLEIGIPYSDPIADGPVIQASYTRALTSGIRLSDIFRTFSQTLPKLPVPAVAMVSY
                     AIVLRQGLGAFLDRAKDCGLAGLIVPDLPIEECGELRERCLANGLDLVQLVTPTTPRT
                     RAVAIAQLSTGFLYYVSVAGITGERNELPPSVVENVTWLRQQTPLPVCVGFGVSRPEH
                     VRMIAAVADGIIVGSAFVRRIAEVPQKGEETVIQEIRQFARSLITAMSNQ"
     gene            complement(3632..4834)
                     /gene="trpB"
                     /locus_tag="PLD05_02965"
     CDS             complement(3632..4834)
                     /gene="trpB"
                     /locus_tag="PLD05_02965"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOP76420.1"
                     /translation="MRVTTNVPDQQGRFGKFGGRFVPETLMFALDQLTEAYEKAIADP
                     EFHQQLEYLYRHYVGRPSPLYFAERLSRLCGGAKIFLKREDLNHTGAHKINNALGQAL
                     LTIRMGKRRVIAETGAGQHGVATATACARFGLECVVYMGEEDIRRQRPNVFSMRLLGG
                     TIIPVTSGSKTLRDAINEALRDWMSSVETTHYCLGSVTGPHPFPRIVRDFQSIIGRET
                     RAQCLEQIGRLPDWIVACVGGGSNAAGMFYPFIEDDVALLGVEAGGRSPQLGDHAATL
                     SYGQPGVLHGTFSYVLQDEDGQTANVHSISAGLDYPGVGPEHSYWKETGRVTYTSVTD
                     QQALEAFQTLARYEGILPALESAHALAKAMELARSLPPDKVIVVCLSGRGDKDTQEVA
                     RLLGQQFA"
     gene            5081..5548
                     /locus_tag="PLD05_02970"
     CDS             5081..5548
                     /locus_tag="PLD05_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76421.1"
                     /translation="MTGSDTFLDPGFYELVHQILSNSQRRVLRERRIRPRFSFDVKQR
                     IAPWTNSRPEEKDFIPVRCFDLNTNGVAFFLEEPPSFRRLIVELSCPQRKVYLESEVA
                     HWEYVYLYPCGDIVPEEVVNELDEEERRDLGEAQRLVLVGCRFLARSKLCHEK"
     gene            complement(5684..8404)
                     /locus_tag="PLD05_02975"
     CDS             complement(5684..8404)
                     /locus_tag="PLD05_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP76422.1"
                     /translation="MVPSTHDGSRSLVRDGLAPHVKAFRDRLWGESQAAISEEYSPGK
                     GLQAALSLCRLRDHILHQLWKIHSSDKADAVWNEETDFYAVMALGGYGRGTISLHSDV
                     DLLILTTRREPSGWKGFISSYFRDIYDLGLKLGFLQLRARAALQKILQDPHFCTAVLS
                     ARFLWGHRPVFEAFQEAIRNKLRRNLKRVLASIERARCEERDRFGKTVFLLQPNIKRS
                     PGGLRDIQFIDWIRYFWELWEPVSSIPITDVLLREELEALSDTEDFLLQIRHLLHLWA
                     NRPEDVFSRSDQWLLAERWHCQATVNLLPVEVLMRDYFRRTEQVVLVAKRLAHALEHQ
                     LTPPKGIAKRSKGRTLVEVKGRLWLTESGREMLKRGWTGLFDLFEACQQLDLPFDASV
                     WIEIWKFRSLFASPPTSEIRQRFLQLLQSPHQTASLIRGLHQVHLLDNFLPDMEHARG
                     LLQFNQYHKYTVDEHCLNALEAATALAYQPGLLGETYRKTINKKILHLAIILHDLGKG
                     LAADHSEAGRSIARRTAEYLALEPDDAELLEFLVANHLRMNHLALRRDINDEQLIVQF
                     AAEIGSPDVLRMMYILTATDLMAVSPDNWTAWKADLLAELYQRTMRYLSDSGDVILTD
                     LDLQERRKAVENALGHDVARPFFSRQVRELPAGYLLATDVETIVRDLRWLASLGNCPV
                     DVRIEYRPSSQTLNLTIATRENVIEGIFHRLTGAITSCGLEILAAQIYTLADGWIIDR
                     YTVRDPYFVGEPPLDRYQDIRTAIVSALREKQFQPVFPKIWGMSPSSRVQVPQARVRV
                     QIDNVSHSDFTLIEVFANDRPGLLYAISRELFMAGCFIWRAKIGTFLDQVVDVFYVTD
                     QKGQKLLDPEHVQILRANLLQVVQSGWEEGSDTPAGSSSNPPTVRAHHDP"
     gene            complement(8407..8748)
                     /locus_tag="PLD05_02980"
     CDS             complement(8407..8748)
                     /locus_tag="PLD05_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOP76423.1"
                     /translation="MKKVEAIVRHFKLEDIKAALMEIGIQGMTVTEVRGFGRQKGHTE
                     TYRGTEYSVDLIPKVKIEVVVPDADLEQVLESIIQAAQTGQVGDGKIFVSDVSQAIRI
                     RTRETDDAALR"
     gene            complement(8795..8977)
                     /locus_tag="PLD05_02985"
     CDS             complement(8795..8977)
                     /locus_tag="PLD05_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76424.1"
                     /translation="MALLLFYAIKTTVGLRVKPETELAGLDISEHGILAYPEAFVVDT
                     GMPLGGSAALAQPRKK"
     gene            complement(9141..9758)
                     /locus_tag="PLD05_02990"
     CDS             complement(9141..9758)
                     /locus_tag="PLD05_02990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOP76425.1"
                     /translation="MMSAKIYTRSGDAGETGLHRGPRVGKDCLRIEVCGALDELNAVV
                     GLIRATGVPQVAEVILDEIQRRLFEMGTEIATPEAERHGCRFITPDHVKAVEAEIDRL
                     EEQLPPLNHFILPGGTPAAALTHFARTVCRRAERRLVALVREALDLGTPLSPTLLAFI
                     NRLGDLFFVLGRWLNQNAGRPETIWLPPSKPATESKNENKEADCP"
     gene            complement(9850..11433)
                     /locus_tag="PLD05_02995"
     CDS             complement(9850..11433)
                     /locus_tag="PLD05_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOP76426.1"
                     /translation="MAQPLSQISHWCENTNWLKRLAQYSIVCLLVVSLGCSRGEDTRK
                     AEQQKASPQDTSHTSEEVEIPLELWYVNYVLGERVGYERQSFDFADEDGRRLRKVSGE
                     SCLRVKRASTIIEMRIRHTCWETLEGWPVRFEVETDQGESTHRTKGEIRDGQLIVETE
                     VAGQAVQKSLPCPANCRGFLGLQESLFEKPMSPREERDLTTLVPGLNDIAYMTLKAND
                     YTSLQVGNRDVRTLSIQFTMTLNSGGIFRGQLWVDERGIIVKQETDSPPMTTILATRG
                     DALEQSPDPRLDLVRDIRIPVKGAPFPVERAAQAVYKISCDTEDLSQLLPSDCHQTVK
                     RLGPHTVELDVHPLSLSPEMPDCHESADPSTTQATLWVQSNAPEIIRMAEMAASGISD
                     PQDVAPALAKFVYRTVRNKGYDHAFLSALEVAERKQGDCTEHAVLLAALARARGLPAR
                     VAVGLVLQGDAFYYHMWTEILVNGCWVGFDATRERGIITPAYIKLGHNSLGTPDGLGA
                     FLPAMKLIGQLTIEIVQSK"
     gene            12100..12795
                     /locus_tag="PLD05_03000"
     CDS             12100..12795
                     /locus_tag="PLD05_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76427.1"
                     /translation="MGFLKKFIGSVLREGSQAPSRSSFEHLSEEELEAHLRIAKYGNF
                     TLTDAVRPSYDLQVVPCEGFRHDVYRSDNGQTVIPVLMASVSKEKLFDTFIELLDPLG
                     EEVDVVLETSHHRDRPGHHDLYREHIDMPVLKSILWEFEDVLLNDGCTGIAVLNADIP
                     LEVQFDEHKLLICYGSDLRPFERILKSHGIPNNEQIRFITEAEHVHSSTEHYSRRFEE
                     LKTYLGMDEGCYV"
     gene            13004..13942
                     /gene="xerD"
                     /locus_tag="PLD05_03005"
     CDS             13004..13942
                     /gene="xerD"
                     /locus_tag="PLD05_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOP76428.1"
                     /translation="MSPARRRLKPHVASPTRATAAWEDSFVHYLSAECHLSENTVAAY
                     RRDLRRFLEWLNARDLKGLTVKDLSDYASWLHEKGLAASSLARHIVSLRLFLRYLQLE
                     GVLTTNQAELLASPQVWERIPKVLTPQQVDALLDSPKPFDPCYRRNRAILEFLYATGC
                     RVSEVANLRMEDVHVEERYCLCRGKGNKERVVPLGERAIMRFQEYLEHERPKLAKKNG
                     GDPPWAFLSYRGRRIRRERIWELLKLYALRAGLPGDISPHVLRHSFATHLLAGGADLR
                     LVQEMLGHASIATTQIYTHVDFARLKAIHRRFHPRA"
     gene            complement(14165..14659)
                     /locus_tag="PLD05_03010"
     CDS             complement(14165..14659)
                     /locus_tag="PLD05_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP76429.1"
                     /translation="MKFRGSGKHVGGKIELMMTPMIDVVFQLLTFFIMTFKIVAPEGD
                     FFVKMPLSAPSAGVPSDLQLPPIKVRMTAGPGGELAGLYLSERRLANFNELREQIRAL
                     VGDSGGPGSAGDAEVELDCDYDLRYEYVIRAITAVSGYVSPDGQIVRLIEKIKFAPPR
                     SEGI"
     gene            complement(14661..15101)
                     /locus_tag="PLD05_03015"
     CDS             complement(14661..15101)
                     /locus_tag="PLD05_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP76430.1"
                     /translation="MPRRAARHIPEAEAEMTPMIDMTFQLIAFFMILINFSEGDQNQL
                     IRLPASELAKPPDTAPAWPITLQMTSKGTVFYGVDELTLENVQPVLEIEKELIRRRGG
                     DFRNTTIIIRADRFAKTGRVQELIRICQSTGFEKFTLRAKQESP"
     gene            complement(15179..15931)
                     /locus_tag="PLD05_03020"
     CDS             complement(15179..15931)
                     /locus_tag="PLD05_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP76431.1"
                     /translation="MPQLPGWLQASATAEEAADDQLDEPAPAAEAPSEGQVAAPEKSV
                     LAWFFESLGWTYTLAFLTISFSFVALLVMNLVACRREAVMPQALIEGFETLFNEKRFQ
                     EAYELAKNDESFLGQVLSAGLAKLSAGYPQALEAMQEVGEEQAMRMEHRLSYVGLIGT
                     ISPMVGLLGTVDGMVRSFSVIANSTTTPKPSELARGISTALITTLVGLTIAIPAVIVY
                     GIFRNRIARLVLEVGIVSEGLMSRFSSVAPKK"
     assembly_gap    15973..16072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16073..17046)
                     /locus_tag="PLD05_03025"
     CDS             complement(<16073..17046)
                     /locus_tag="PLD05_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76432.1"
                     /translation="MQAFVKTVFLVVAVTFPALIVSAADVVQQGNQVVAGKITQMTRT
                     EVVIETTSGPRTIPVNQIQSISWDGEPATLKNIRMAMQVSQYEDVITALERIKLDTVT
                     RPEIRQDIEFYRAAATAYLALRGTGSIDEAGKLVADFALNYPNNYHVWEATRLVADLL
                     VAKGTVDKAVEYYASLSQAPWPEYKAQAQTAIGWAYLGAGKIDEAEKAFDEVLVIQLS
                     GDDTPKVLATIGKARCLAEKGQTEPAVALIQDLLKRPDLTENAEVMGRAYVTLGLAYR
                     KANDAKRAIIAFLHTDLIYFRHSPSHIEALQNLLELWPQVGHPERAAEV"
     gene            complement(17109..>20096)
                     /locus_tag="PLD05_03030"
     CDS             complement(17109..>20096)
                     /locus_tag="PLD05_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76433.1"
                     /translation="LEALKSRGYYDTAVEYLDMLASDPTCPAELKEVIDYEAGVALVA
                     AAQTARNPQKRDQALKESLERLRKFIQEHPQHALADAARAQLGQGLVEQAKALLQGIE
                     TKKPDEVSRLRGEARKLIEQAKQHFDAYEKSAYERAKQFQGKVIDARNEQLAREREQA
                     YAEYLKARMFTIGAIYQLAQTYGKEEKQRAELLKQAAGEYEKFYEKYRDFSAGFFARF
                     YQGICAQELGQDNVAIDIFKETLTVPGDLPEIQRLRTEALARLIQIYRKQNKHQDIVS
                     AAVSWEEAVRPDVRNTDWGLEILYHGALSAIELAKSAEKNKEGRTAQQQRALAQRFLK
                     IVASVPGQYRLDAQSKLGELLGEERTIEDSPVVDFADGKARGEAAWGRFLAAMAQLDK
                     GEGDRTQIRQEANRARDQAISFYQKAIALYKDQPIEELNAIRYQLIYLFWDAGALEKS
                     AVLSEFLARNYPQNAGSQKAVEIAVKVYRNLFAQARKDGQDGSFELEKMRQLTEYALS
                     QWGNEREASECVLTLLESFFDLNLMNDAKEFLERIPLESSGRARAELRLGQVLWTQYV
                     KLAASQDARPSPEESDAILQEARRYLEQGLQKIRSGGGEVDYLVEYSVLLLAQLLVNT
                     GETDQAITLLEDATLGPLTLIRQGHAAVENAKLREEVMKVALRAYVGSQNLDKAFEVQ
                     EQLEKLVAGSGDAEATKRLTAVYVGLGRQLEEQLARLRSEGKTAEMEKVLEGFTKFLE
                     AIQERSASVDFRALNWVAETFLGLAKGLDPGGPQVPEQAKEYYKKAVETYIQLIKRCE
                     AEPSFGPEDAPLFLRVRLAAALRGWRLFDKAMEAILFVIDKQGKENRLDAQLEAAQIY
                     QEWAEQPGKEQYYEYAIRGGHEKNGRYLIWGWSGIARRVASDYNRFLDVFHQARYNLA
                     YCRFKLAQKATGAKRDQLLKEAELDIQRTYLLYPSLGGKEWYAKYDKLLRTIQQLRGE
                     KNPQGLKAFESRPRATPASSKAG"
     assembly_gap    20097..20196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20620..21762
                     /locus_tag="PLD05_03035"
     CDS             20620..21762
                     /locus_tag="PLD05_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOP76434.1"
                     /translation="MPIFTRYVTSELLKIFLTALLIMTLLLALGIGAREGISRGLPPL
                     LVMRMIPYLLPEILGITIPIAVLLAVSQVLGRMAGSNEIIALRAAGVSPSLVVRPTVI
                     FAFFVSLFTVWTYDLAATWGRPGARRVVVESVEQIAYGMLRTRKSYSSQKFSLVVKDV
                     VGKRLISPIITVQVQPESPAITLTAEEAELFTDRKAGALTIVCRKPVARIEGEATVTL
                     PETYWYPIPFETSYEPLHRDWLAMSEIPAAIRKLEGDARQLKLLLTEGGASEEQRRRW
                     NEQLQNLELQIRKLQTEPYRRWANGFSSLCFVLLGCPLAMYRRSENFLAIFFLSFLPI
                     VIVYYPLLMLANGLTSSGILPAVGFWIANLGIAIPGVVLLYYYCET"
     gene            complement(21860..22243)
                     /gene="acpS"
                     /locus_tag="PLD05_03040"
     CDS             complement(21860..22243)
                     /gene="acpS"
                     /locus_tag="PLD05_03040"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOP76435.1"
                     /translation="MRILGIGTDITECLRIARMIDRHGEIFLQRVYTPAEIAYCQNRK
                     QATQHFTGRWAAKEAILKALGTGWIRGISWLDVEIVNRPGGQPEVILRNGAKKIADQL
                     GIEKIQISISHCRTHAVAFAVAVGD"
     gene            complement(22383..23351)
                     /gene="trpS"
                     /locus_tag="PLD05_03045"
     CDS             complement(22383..23351)
                     /gene="trpS"
                     /locus_tag="PLD05_03045"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOP76436.1"
                     /translation="MIVVSGIQPTGRFHWGNYFGAIKQYVELQDQADAAYYFIANLHA
                     LTTVRDPQVLRQNTIDAAIDLLALGINPDRATLFIQSDVPEVTQLCWLLMTCTPLGLL
                     ERCHAYKDKRAKGLPADAGLFTYPVLMAADILAYDADTVPVGEDQVQHIEVCRDVASS
                     FNHLYGNVFVMPKAKIMENAARVPGTDGEKMSKSYNNTIGIFDDPKTVRKTIMRIVTD
                     SRPIEQPKAPETDHLFQLYSLFVGEREREEMAARYRRGGFGYGEVKQALADVAEKYFA
                     EAREYRKELESKPQFVREILGDGAAMARKKAAEVLQRAQNACGIKY"
     gene            complement(23432..24397)
                     /locus_tag="PLD05_03050"
     CDS             complement(23432..24397)
                     /locus_tag="PLD05_03050"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOP76437.1"
                     /translation="MIEANKECDLSIRIGGLVLPNPVMFASGTFGNIVDGYPIANLSR
                     LGAIVPKTVTFSPRIGNPPPRTVETPAGMLNSIGLDNDGLMAFLERRLPALSTIGPPV
                     IVSIAAKDEDEFVKMAEILDAAQGVSAVELNLSCPNVAGGIDFASDPRKCHVLVARVR
                     QRFSRAILAKLTPNTGDIATIARAAADAGADAITAVNTLLGLAVDWRRRRSRLGGFFG
                     GLSGPAIKPVALRCVYQIAHAVDIPVIAAGGVTNVDDAMEFFVVGASAIQVGTASFFH
                     PMAAWEIIDALPSALRELGAYSIREIVKTLCEPRKACDLRKTREC"
     gene            24783..26357
                     /locus_tag="PLD05_03055"
     CDS             24783..26357
                     /locus_tag="PLD05_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOP76438.1"
                     /translation="MNFEHKNDFPFCCGCRYPHSKQSVEDRQTTGVTRRDFLGAAAGG
                     VVASAVLATTTHGAWETASHRPPSVKTLQVQPVFNCHIYPRQEATSWRVTGAIQDEAE
                     LREEENRIRADLKKLQEMADFPMEIRPLVTVGTVEEANAVASQPCDVMLVFAARRNVP
                     VLEALIRPERWNLMFVRHRSGPLYYMYIGVHVHYLRKRRDFFAEPAMTIDDVVVDELP
                     ELLWRLRALFAVKNTLGQRIVAIGNPAGWGADGAQAPARAKEVWKFEYITVPYPELEE
                     RLKQARANERLVQHCQQQAEAYLRDAQVSLETERGFVERAFVLTEVFRTLLDEADANA
                     ITVHNCMTTIMPVSRTTACLPLTLLNDDGYLAFCESDLVAIPAGILLHYVTGRPVFFC
                     NPSFPHKGIVTVSHCTAPRRMDGKSLAPARILTHYESDYGAAPKVEMPRGQEVTVIDP
                     DFNGQRWLGFEAAIMDSPFFPICRTQLDLKIIGDWQRLIQEIRGFHWLLAYGRVLKTF
                     GYAVRKAGLDWLPIEA"
     gene            complement(26632..30720)
                     /locus_tag="PLD05_03060"
     CDS             complement(26632..30720)
                     /locus_tag="PLD05_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOP76439.1"
                     /translation="MIKCVSIFVALVGVIFSVSQTGFAGSVAFLIPAARTTPLGQEAQ
                     AALAWLQANRDCRVIYLEGPEQFVNDRGEPVPLTEFQIVWCHQGDLPTISADLQNPGT
                     LEALRNFVSQGGRLFLSGAAARLVIDMGLETGVMRTASPGRDGFQSALIPVVRDHPIF
                     AGLTARPGSPIFFTSGGYPAFADFYRGGGPKTGRLLARANYADENPLVEYRLGKGRVI
                     ILGWRIPNYALANDVHRRALDRLTANILDYLADDTKWVDWQESETSAISMAKAEIPST
                     SELAALKAAIDDLITSFGDRYREGHLFQDRWEKLKTACDDLRRRNSVDEQAIQQVRTS
                     FLELQQEALLANPLLDFDTLLYVERKEPKLGLPANWESHSSLPPTGYDNRICVISLRR
                     RNEPPQVLFEPAEGRYVGEIDLHFDAKRLLVSMPGSFNRFQVFELDLEHQEGRWRVTG
                     LRELPLILEPDIDNYDACYLPDDRVVFTSTAPFVGVPCVYGQSHVTNTYRWDPKDGHI
                     RQLTVDQEHNWHPSVLNNGRVLYLRWEYTDLPHAHSRILFSMNPDGTNQMAYYGSNSY
                     FPNSFFYARAIPGHPTKVVGIASGHHGTRRSGRLLIVDPAVGRHEAQGVVQEIPPTHQ
                     TIEPTIRDELVDGVWPQFLTPWPLSEKYFLVSCKPSPEQSWGIYLADIFGNLVLICEK
                     PGFALLEPIPLAPRPRPPVIPDRTDPLQKEALVYLVDVYRGPGLKGIPRGTVKKLRLV
                     SYEFSFRGMGGLLGAIGMDGPWDIKRVLGTVPVQEDGSAFFRVPAYTPIAVQPLDAEG
                     RALQIMRSWFTAMPGEVLSCIGCHEHPNDAVPNLQSLAVSHPPAKIEPWYGPPRGFSF
                     AREVQPVLDRYCVSCHNGQSESPGAFDLRGNVPLEGWSSAIAGHVSDKVGGKFSVAYA
                     NLHPFVRRPGIESDIHLLSPMEYHAATTELVQVLKKGHYGVNLPPEAWDRFYTWIDLN
                     APYHGTWSEIVGPETVMPLLARAREMRKRYTGMDDNWEEIPTITVFGTTIGTTPDEIT
                     RNDGNKLVSFTTEERSSGEKPPQDVTVPKLDDWPFSYEVARERQEKLKDWQRVITIGP
                     GVTVTMVRIPSGRFVMGNAEGEIDERPPCVVEIPHDFWISTCEITNRQFAVFDPTHDS
                     HVESLHGYQFGYHGFPLNQPDQPVVRVTWHEAVAFCDWLSNRQGVRFRLPTEAEWEYA
                     CRAGTAEPFWFGSLDDDFSPYANLADQRLSEFVMDTYIQVRTIANPNHFDDWILKDPR
                     WNDGGLVSVSVGSYRSNPWGLYDMHGNVWEWTLSLYRDYPYADNDGRNDMAASGLRVV
                     RGGSWYDRPKRATSSFRLAYPPYARVFNVGFRIVAESLPTAE"
     gene            complement(31055..31273)
                     /locus_tag="PLD05_03065"
     CDS             complement(31055..31273)
                     /locus_tag="PLD05_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76440.1"
                     /translation="MRKLIVAILGAFLLSSVVGCVVPIYSADPDRRVQQLIYTSEGLR
                     LMLDEWERAWMLDHPDHMTPYRTHGGII"
     gene            complement(31459..31755)
                     /locus_tag="PLD05_03070"
     CDS             complement(31459..31755)
                     /locus_tag="PLD05_03070"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOP76441.1"
                     /translation="MTKKDIVRRIAEELGVSQTLTREIVQRTFDAIIDTLTTEKRIEL
                     RNFGVFRIKTRRPRRAINPRTKESLQVPEKYVVRFKPGKELKKRLKAMSKKPRP"
     gene            complement(32156..32590)
                     /locus_tag="PLD05_03075"
     CDS             complement(32156..32590)
                     /locus_tag="PLD05_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOP76442.1"
                     /translation="MQRVSKKTALPWYHEGLRFECTGCGYCCSGQPGYVYVNQEEIKA
                     LAEFFRLSPAEFEERFVRTTGKAKSLIEQPNGDCVFLHPLTRKCLVYELRPRQCRSFP
                     FWPSILKTKRTWEEICRTCPGCGQGPLHSLEEIDRRKQMIDI"
     gene            complement(32627..>34576)
                     /locus_tag="PLD05_03080"
     CDS             complement(32627..>34576)
                     /locus_tag="PLD05_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOP76443.1"
                     /translation="GVIYLQPFFDTQDRHLFRKMKQVADEVYGYVLSVGGGIGPTLGC
                     GLARTPYMKQQWGHLYPIFEKIKKAFDPLNILNPGKIIETPDTGDIWKLARGRLFVGF
                     EAPSSEDDGNEDGAGFQRTGVHTTQGATSLPETQSRGERQDDIPAPAASTAGVIDFGD
                     RAAAEIDSQHYSYSPPSELTLNAPPTSQQDHIPGRLDDRSENLPRILESQLDWQPDLV
                     ANAVSRCSGCGDCRTQDHRVRMCPLFRALPWEECAPRSKSSLMRAVLTETLPLQSLTL
                     QDFREICDLCVHCHSCRIECSAQVDIPLLASEAKAAYTLAHGSAFADWLLNRIDGLAS
                     LLSRFAPLVNWGLANRQVRWLLEKLTGISQVRKLPRLHAVSFLHRAARKKLHRLDRLP
                     GRKVVYFVDVYANWFDPALAEALVAILAHHGISVFVPLQQRSAGTAAIASGEMNLARR
                     HARLNVAILAEAVRQGCEIVTTEPAAALTLIHEYPQLLQDEDSQLVAKHTHDAGEFLL
                     GLHRDGQLRTDFQNLPLRLGYHLPCRLRALGVGQPGRELLELIPGVTVEGLPNTCSGM
                     AGMFGIKAKNFRTSLRLGWSIISHLRDPAIQAASTECSACRIQLEQGTTKPTLHPIKV
                     LAAAYGLWPGGLQRLLRPGSSFVLS"
BASE COUNT         7748 a         9276 c         9430 g         7922 t
ORIGIN      
        1 ggagagcgcc tgctttgcaa gcaggaggtc gtcggttcga gtccgatcat ctccaaatcc
       61 ttattggtgg gcaggtctgt tgtaccgcaa gtgaatggct tgaacttgcg catcgttagg
      121 aattgacggg gcatgtcgcg ccggtactgt ctttttaggc agcgctattt tgtggcatgt
      181 ccagccacgg gacgagccgt tctactgatc ggcgcgcgga gagaacccgg cagcggtggc
      241 tttccgaaaa caggcaaagc acgagcaagt gcgatcaata cctgattcgg cgctcctctc
      301 ggtttcaaga agccttgtac tgcttttctg ctggccggct agagaccgag acatctccac
      361 caaggatttc gcccattatt tatttttagc cttgggaaga tgtccgagat attgcctact
      421 ttcccaaaag ccgcgttctt tcgcacactt cttcagcaaa aggaaactgg tcacaaaaag
      481 taccatcagg gggtggccga gagtttgtct agaaacgggt cgccccgcga gatttaactt
      541 aatggcacac gcgccgtgaa ggtccaacct tccgggaacg caatgttttg cgggagactc
      601 agacggaacg cacagaagct ggaagatccg gacgagcgta ggcccttttt cctacacgca
      661 cacaacaacg tgagccgacc gacctgccaa tagccggtgg ctctttgtgc acatctccca
      721 ctgcgcggcg cccatggttg tgtaacgatc accccttgcg cgcgtgatgt gtgataaaca
      781 aagcggaggg cacgggactc gaacccgcaa cccctttcgg ggcacctgat ttcgagtcag
      841 gctgctcacc aattcgcata ccctccggag tcgcttaaaa tacgggccga cccagtcagg
      901 tcgtgcccgt ccaataaaaa tgtatgttac ggcgaccgat ttttcaatgg ggatgggcgc
      961 gatgacacat cgtggggccg tgaggggcgg gattatgttc tgatctaggt tcgcggccat
     1021 cccttgtaaa cggctaaatc ggtcaatttt caacaacatg ccgcctgtcc gagttacgaa
     1081 cggagagccc ttaaatctgc ttggccggta ctccaaaggg gtgtccattt gtggaaaccc
     1141 ggggcatgtc ttctggaagt tctcgcaatg tactgtttca gctcagtgga ccgtgagcaa
     1201 aaacgggatg aattgcagtc tccacgtagc ctgtgtgcat ccgttctcgt cacctaacat
     1261 gtcgttccga gaacgacgtt cgccacgcca aacccgcccg acaccttggg actattccca
     1321 acagtcggta gaataaggtt tttccgcgtg ttgaatggaa aggccgctct ggctgtgcgt
     1381 tcaatacgac acgattcgta aaaatatgac ccgatggagt gacgagcatg gcaaagtgtg
     1441 ttctggccta ttcgggggga ctggatacgt ctgtcattct tagttggctg gtcgagcaag
     1501 ggcatgaagt tcacgcggta tatgtcgatc ttggacagcc gtgtgaagac cgggagacga
     1561 tccttgccaa ggctcggcag attggcgcga agaccgccca aatcattgac gctcgggagg
     1621 agctttgccg cgattttgcc tttccggttt tgcagtggca agcccgatat gaggaccttt
     1681 atctgatggg gacctcgatt gcacgaccgc ttatcagcaa ggtctgcttg caagtggcgc
     1741 ggcagcacgg gtgcgatttg ttcgtccacg gtgccacagg gaagggaaat gatcagtgcc
     1801 gttttcaatt ggcggcagag gcacttcagc cgggtatccg agttctagcg ccgtggcggc
     1861 tggccgaatt ccgggaacgt ttccccgggc gaaaggaaat gattgcgtac tgtgagcagc
     1921 gggggattcc tgtgaaggcc accgcttcca aaccgtatag ctcggatgag aactgcttgc
     1981 atatcagcta cgaggccggt cgccttgagg acttgacggt caacggagtc gagttgatcg
     2041 aatttggaat gaccgtacct ccgcagaagg ccccggacca gtcagagaag gtgacgattg
     2101 catttgaatc cgggattccg gtgagcgtga acggagagaa actgtcgcct tatggcatcg
     2161 tgaactgctt gaaccggatt ggcggtcgtc acgggattgg tcttgtcgat atggttgaga
     2221 accgttttgt tgggatgaaa agccgcgggg tgtacgaggc gccggggatg acccttctct
     2281 attttgccca tcggcaagtc gagcagatca cactggatcg ggacgtcatg catcttcggg
     2341 atcggttggc cccggaggtg gcggaacttg tgtactacgg gttctggtat tcggccaaga
     2401 tggacgcgtt cttggccatg attcgagaga ttcagaaacc ggttacgggg gagattacac
     2461 tggtcttgta caaagggaat atgatcgtcg atcgccggtc cagcccgaac agtttgtatg
     2521 acgaggggat agccaccatg gaaggcggcg gcgactataa ccagagcgat gcggaaggct
     2581 tcttacgcat ccaaggattg ccgcttcggg ttcaagcgag gattcggccg cgctcgtttt
     2641 gatggaagcc cttattctct cgctgtgaga gaaaagagaa atgcagcctg tttcccgcgg
     2701 gagcgtaatt tgtgcgggac acgcacgtac accccgcgtt acttgatgcc tggggttact
     2761 gattcgacat ggccgtgatt aacgagcgtg cgaattgccg aatctcctga atgacggtct
     2821 cctcaccttt ttgggggact tcggcaatac gacgaacaaa ggccgagcca acaatgatgc
     2881 catcggcaac ggcagcgatc atgcggacgt gttcgggccg gctgacgcca aaaccaacac
     2941 agacgggaag tggtgtctgt tggcgtagcc aggtgacgtt ctcaacgaca cttggtggaa
     3001 gttcgttgcg ttcccctgtg atgccggcca cggagacgta ataaagaaag cccgtgctca
     3061 gttgggcaat tgcaactgcc cgagtgcgcg gggtggtggg tgtgaccaat tggaccagat
     3121 caagcccatt ggccaggcaa cgttcccgta attccccgca ttcttcaatg ggtagatcag
     3181 ggacaattaa tcccgcgagc ccacaatctt tcgcccggtc caaaaaggcg cccaacccct
     3241 ggcgtagcac gatggcataa ctgaccatgg caactgcggg cacggggagc ttgggtagcg
     3301 tttggctgaa cgttcggaaa atgtcggaga gccgaattcc cgaggtaagg gcccgcgtgt
     3361 atgaggcttg aatgactggg ccgtcggcga tggggtcact gtagggaatg cctatttcta
     3421 aaagggagca cccttcatcg gccagggtgt gcatgagtct aacggtgaag tccagatttg
     3481 ggtcgcctgc cgtcagaaat ggcatgaacg cccttttacc ttccttccgc aaagctttga
     3541 acacttcact gatcctagac atataatgct gcttttcccg tgcagtcatc ttattgaaag
     3601 ctggtgtgtc tttcttttgg tctcaaatca cttacgcaaa ttgctggcca agaagtcgcg
     3661 cgacctcctg agtatctttg tcaccgcggc cagagagaca gacgacaata accttgtcgg
     3721 gcggcaacga gcgtgcaagc tccatggcct tggccaaggc gtgggcactc tctagagcag
     3781 gaaggattcc ctcgtatcga gccaacgttt gaaatgcctc gagggcctgt tggtctgtga
     3841 cactggtgta ggtcacacgt ccggtctctt tccaataact gtgttccgga cccacgccgg
     3901 ggtaatcgag acctgccgag atcgaatgga cattggccgt ctgtccatcc tcatcttgca
     3961 ggacataaga aaaggttcca tggagaacac ctggttgccc ataggaaagg gttgccgcat
     4021 gatcacccag ttggggcgaa cgcccaccag cttcgacccc caagagggcg acatcgtcct
     4081 cgatgaaggg ataaaacatc ccggctgcat tgcttccacc accgacacaa gccacaatcc
     4141 aatctggcaa gcgtccgatt tgctccaggc actgtgcgcg ggtttctcgt ccgatgattg
     4201 actgaaagtc gcggacgatc cttgggaagg gatgagggcc ggtgaccgat cccagacaat
     4261 aatgagtcgt ttcgacagaa ctcatccagt cgcggagtgc ctcattgatg gcgtccctaa
     4321 gggtcttgga accgcttgtg accgggatga tcgtgccccc gagaagtcgc atgctgaaga
     4381 cgttgggacg ctgccgccgg atatcttcct cgcccatgta aacgacgcat tctagcccaa
     4441 aacgggcaca tgccgttgcg gtggcaacgc catgctgacc cgcacccgtc tccgcgatca
     4501 cgcggcgctt tcccatgcgg atcgtgagca gggcctgtcc cagggcatta ttgatcttgt
     4561 gggcacccgt gtgatttaaa tcctcccgct tcagaaaaat cttggcaccg ccgcagagtc
     4621 tcgacagcct ttcagcgaaa taaaggggag aggggcgacc gacgtagtgc cggtaaagat
     4681 actccaattg ctggtgaaat tcggggtcgg caatggcctt ctcgtatgcc tcggtcagtt
     4741 gatcgagcgc aaacatcaag gtttcgggca caaatctgcc cccgaacttt ccgaatcgcc
     4801 cctgctgatc agggacgttt gttgtaactc gcatgggaga atcgccttta atccacacaa
     4861 caaccttatc aaggaccgaa aaaaccaatt ataacacttg tgcagcacgc caaaccaaaa
     4921 atcttatcgt aggttagaat tgagatgtca atcaaggcgt catcgaggcg gtgagagttg
     4981 aaagatcggc cgggcatggg tgactttgcc aaatagaaca cactcgacat atgggatgat
     5041 cgtactttcc gggaaatacc gtgcggggac atgaaaagct atgaccggat cggatacgtt
     5101 tcttgacccc gggttctatg agcttgttca ccagattctg tcgaactccc agcgacgggt
     5161 gcttcgagag cggcgaattc gtccccgttt ctcgtttgat gtgaagcagc ggatcgcccc
     5221 gtggacgaac agcaggccgg aggaaaagga ttttatcccc gttcgttgct tcgatcttaa
     5281 cactaatggt gtcgcctttt ttctcgaaga gccgcctagt ttccgacgac tcatcgtcga
     5341 gctgagttgt ccgcagcgga aggtctactt ggaatcggaa gttgcccatt gggaatacgt
     5401 ctatctttat ccgtgcggtg acattgtgcc cgaagaagtg gtgaatgaac ttgatgagga
     5461 agagcgacga gacctgggag aagcacaacg tcttgtcctc gtcggctgcc gcttcctcgc
     5521 tcgatccaag ctgtgccacg aaaaatagac gcacaagtgg tgggcccatg aggccgcgtt
     5581 cctgagttgt cccatcgagt agggggagcg gggcgccgat ctttggtcaa gaacaaggat
     5641 gggagatttc catcattttt agccctcaca gtgaggcgat gtgtcacggg tcatgatgcg
     5701 cacgaacggt aggtgggttg gaagacgaac ccgcgggggt atctgagcct tcttcccagc
     5761 cagattgcac tacctgaagg agattggccc gaagtatctg cacatgttcc ggatcgagca
     5821 atttctgccc cttttgatct gtgacgtaaa agacatcgac cacctgatcg agaaaggtcc
     5881 caatcttggc acgccagatg aaacagccgg ccatgaacag ctctcggcta atggcatata
     5941 gcagcccagg gcgatcgttc gcaaagacct cgattaacgt gaagtcggag tgactaacgt
     6001 tgtcaatttg gacgcgaaca cgcgcctgag gtacctggac acgcgacgat ggggacatgc
     6061 cccatatttt tggaaaaacg ggctggaact gcttctcccg aagtgctgag acgatcgccg
     6121 tacgaatgtc ctggtaacgg tccaaaggag gctcgcccac aaaatacggg tcgcgcaccg
     6181 tgtaccggtc gatgatccag ccatcagcca aggtgtagat ttgggcggcc agaatttcta
     6241 gcccacagct tgtgatggcc ccggtcaatc tgtgaaaaat tccctcgatg acattctctc
     6301 gagtggcaat cgtcagatta agggtctggc ttgatggacg atattctatg cggacatcca
     6361 caggacagtt gcccaacgat gcaagccaac ggagatcacg aacgatcgtc tcaacatccg
     6421 tagctaggag ataacccgcc ggtagttcac gtacttggcg tgaaaagaac ggccgagcga
     6481 catcatgtcc cagggcgttt tcgacagcct tccgccgttc ttggagatca aggtctgtga
     6541 ggatgacatc gccagaatcg gaaaggtacc gcatcgtccg ctgatatagc tctgccaaca
     6601 agtcagcctt ccatgccgtc caattgtccg gtgaaacggc catcaagtcg gtggccgtca
     6661 gaatatacat catgcgcagc acatcgggtg agccgatttc cgcagcaaac tggacgatca
     6721 attgctcatc gttgatgtca cggcgaaggg ccaggtgatt cattctgaga tgattggcga
     6781 caagaaactc cagtaattcg gcatcgtcgg gctcgagggc gagatattcg gccgttcggc
     6841 gggcaatcga tcgcccggcc tcgctgtggt cagccgctaa acctttgcca aggtcgtgca
     6901 gaataatggc caaatgaaga atctttttgt tgatggtctt tcgatatgtc tcacccagca
     6961 ggcccggttg ataggccaaa gcggtcgccg cctcgagggc gttcaggcag tgctcatcga
     7021 ctgtgtattt gtggtactga ttgaactgca aaagaccgcg agcgtgctcc atgtcgggta
     7081 gaaaattgtc caagaggtga acttgatgga gcccacggat gagtgaggcc gtctggtggg
     7141 gtgattgcaa aagttgtaag aatcgctgac ggatttctga tgtgggcggc gaggcaaaaa
     7201 ggcttcggaa tttccagatt tcgatccaca cgcttgcatc gaagggcaaa tcaagctgtt
     7261 gacaggcttc gaaaagatcg aagaggcccg tccagccacg cttcagcatt tctcggccgg
     7321 attccgtcaa ccacagtcgt cctttgactt caaccagtgt gcgtcccttg cttcgtttgg
     7381 caattccctt gggcggtgtc agttggtgct ctaaggcatg tgccaatctt tttgccacga
     7441 ggacgacctg ttctgtgcgg cgaaaatagt cacgcatgag cacttcgacg ggaaggagat
     7501 tgaccgttgc ttgacagtgc caccgctctg ccaggagcca ttgatcgctg cgcgaaaaga
     7561 cgtcctcggg gcggttagcc cacaggtgca agagatggcg gatttgtaag agaaaatcct
     7621 ccgtatcgct tagcgcctct agctcttcgc gcaataggac gtccgtgatc ggaatcgacg
     7681 acaccggttc ccaaagctcc caaaaatacc tgatccagtc aataaactgg atatcacgaa
     7741 ggccaccagg tgatcgtttg atgttgggct ggagtaagaa gacggtcttg ccaaatcgat
     7801 cacgctcttc gcatcgggca cgttcgatag aggcgagtac tcgcttgagg ttacgtcgta
     7861 acttattacg gatagcctcc tgaaaggctt caaacacagg gcgatgcccc caaagaaaac
     7921 gggcagacag aactgctgtg caaaaatgag gatcttgcag gatcttttgc aaagccgcac
     7981 gggcacgcaa ttgtaggaat cctagtttca gtccgagatc atagatgtcg cgaaagtagg
     8041 aagagataaa acctttccac cccgagggtt cccgccgggt ggtcaaaatc aagaggtcca
     8101 cgtcggagtg aagagaaatc gtgccccggc cataaccgcc taaggccatg acagcgtaaa
     8161 agtccgtctc ctcgttccaa accgcgtctg ctttatccga tgaatgaatc ttccacaatt
     8221 gatgtaagat gtggtcgcgg agacggcaga gagacaatgc cgcttggagg cctttacctg
     8281 gggaatattc ctccgagatt gccgcttggc tttctcccca gaggcggtcg cgaaatgcct
     8341 tgacgtgagg cgctagccca tcgcgtacaa ggctacgaga gccatcatgc gtcgatggga
     8401 ccatggctac ctgagggctg cgtcatcggt ttcgcgtgtc cttatccgaa tggcttggga
     8461 cacgtcactg acaaatatct taccatcccc gacttgcccg gtttgtgccg cttgaatgat
     8521 gctctcaagt acttgttcca ggtcggcgtc ggggacgacg acctcgatct tgacttttgg
     8581 gatgaggtcc acgctgtatt ccgtaccgcg gtaagtctcg gtgtgtcctt tttggcgacc
     8641 gaaaccgcgg acctctgtga cggtcatgcc ctggatgccg atctccatga gcgccgcttt
     8701 gatgtcttcc aatttgaaat gcctgacgat tgcttctact tttttcatat tgcagttacc
     8761 tcacatgctt tttgtggaag accattgaac acaatcactt cttacggggt tgggccaatg
     8821 ccgccgaacc tcccagaggc atccctgtat caacgacgaa tgcctcggga taggcgagaa
     8881 tgccgtgttc actgatatcg aggccggcca gctctgtctc tggtttgacc cgaagtccta
     8941 ccgtagtctt gatcgcgtag aaaagcaaca gcgccatgcc gaaagaccat taacaatgaa
     9001 cgttttcggg aaaaacagca aatcttccga aaaacaggga aaacaggtgt gaagacgacg
     9061 ggctccgaac gaccggtttt cgcccgacgc ttaattcgta caatgggtct ctgcttaaag
     9121 actgggcagg tccgcctaag ttatggacaa tcggcctctt tgttctcgtt cttgctttca
     9181 gttgctggtt ttgacggcgg cagccagata gtttcggggc gcccggcatt ctggttaagc
     9241 cagcgaccta atacgaagaa caggtcaccg agacgattga taaacgcgag aagagtaggg
     9301 gataacggcg tgccgagatc aagggcttcc cgcacgagag caaccagacg gcgttcagct
     9361 cgacggcaca cggtgcgggc aaaatgggtg agggcagcag cgggagtacc accaggcaag
     9421 atgaagtgat tcaacggcgg aagttgttct tccagtcggt cgatttctgc ttcgaccgcc
     9481 ttaacatggt caggtgtgat aaaccgacaa ccatgacgct ctgcttctgg ggtggcgatc
     9541 tcggttccca tttcgaaaag gcgacgttgg atctcgtcca aaatcacctc ggccacttgc
     9601 ggtacgccgg tagcacgaat cagtccgacc acggcattaa gttcgtcgag tgcaccgcaa
     9661 acctcaattc gaagacagtc tttacccacg cgaggacccc gatggagccc ggtctcgccc
     9721 gcatcacctg accgtgtata gatcttcgca gacatcatga tatcccgatc tgacaaatac
     9781 tgagaaagaa aataaaagtc gtttgcatcc aaacgcggct tgagacacag tctctacgaa
     9841 caactccttt catttgctct gcacaatttc gattgtgagc tgtcctatca atttcatggc
     9901 aggcaaaaac gcacccaagc catcgggtgt tcccaaggaa ttgtgtccca acttgatata
     9961 ggcaggggta atgatccccc gttcgcgtgt tgcatcaaac cccacccagc aaccgtttac
    10021 gaggatctca gtccacatgt gatagtaaaa ggcgtctccc tggaggacta gtcctacagc
    10081 aacacgggca gggagaccac gcgcgcgggc gagtgccgcc aggaggacag cgtgttccgt
    10141 gcagtcacct tgtttgcgct cagccacctc aagtgcggaa agaaacgcgt ggtcgtatcc
    10201 cttgtttctc acggttcgat agacgaattt ggccaaagcc ggggcaacgt cttgcgggtc
    10261 tgagattccc gaggccgcca tttcagccat gcggatgatc tcaggagcat ttgattggac
    10321 ccacaaggtg gcctgggtgg tggaaggatc ggccgattcg tggcagtcgg gcatctcagg
    10381 cgacagcgac aacggatgaa cgtccagttc cacggtatga ggaccgagtc gcttcaccgt
    10441 ctgatggcag tcggagggaa gcaactggga cagatcttcc gtatcgcagg agatcttata
    10501 aacggcttgc gcggcacgct ccaccggaaa tggtgctcct ttgacaggga tgcgaatatc
    10561 ccgaacgaga tcaagcctgg gatcagggga ttgctccaag gcgtctcccc gagttgcaag
    10621 gatcgttgtc atcgggggtg aatcggtctc ctgcttgaca ataatacccc gctcatcgac
    10681 ccacaactgt ccccgaaaga taccgccgga attcagtgtc atggtgaatt gaatggataa
    10741 ggtacggacg tcgcgattgc caacttgcag tgaagtgtaa tcgttggcct ttaatgtcat
    10801 ataggcaatg tcattcagtc cgggcacaag ggtcgtaaga tcacgctctt cgcggggaga
    10861 catcggtttt tcgaacaacg attcctgaag tcctagaaaa ccacggcagt ttgcgggaca
    10921 ggggaggctt ttctggacgg cttgacctgc cacctccgtt tccacgataa gctgcccatc
    10981 gcggatttcc ccttttgttc gatgggtcga ttccccctgg tctgtctcca cttcaaaacg
    11041 aacgggccag ccctccaacg tctcccagca tgtgtgacga atcctcatct cgataatcgt
    11101 gctcgcgcgt ttgacacgca ggcagctctc tccggagacc ttgcggaggc ggcgaccgtc
    11161 ttcgtccgca aaatcaaagg actgtcgttc ataaccgacc cgttctccca gcacatagtt
    11221 gacataccaa agctccaggg gaatttccac ttcttccgaa gtatgcgagg tgtcctgcgg
    11281 agaggccttc tgctgctcag cctttctcgt gtcctcacct cttgaacacc ccagcgagac
    11341 gacgagaaga cagacaatac tatattgagc caaccgtttg agccagttgg tattctcaca
    11401 ccaatgacta atctgcgaaa ggggttgggc catttgtcgt tttatgttcg ctaaaagacc
    11461 tcacgccaaa gccactgtgc gggtttcggt cgggcatcag cttcagtata tcgaaggcct
    11521 ctcgacatag gtcaagggtg acaccgacct gtgatcttct ttgtctgcat catcttgtct
    11581 ggtcgcaaga agcagcacgg ccggaggttc ttgggtaacc gcgagttttt gcgcggtctg
    11641 tgtgttcgtt gcaaatgagt cgtcatgagg ctgtgggcca ttgtctttga agaaacatcg
    11701 ggccgaattt ggtgctagag ttcttttagg agacgcgatg cgggacggga aacggctttt
    11761 ccagtagcct ggtcacgact tgtgcccata tcagtctctt ccccgagacg aaagcgtgtc
    11821 gcggaaagtg tggcgggatt tagttgatcg tacgggtttt gcgggataac caaaatcgca
    11881 agcgaaaaaa tggagagaac gagacgtgat ggagccctag atatggccta ggttacagca
    11941 ggggacggcg tgattggtcg cccaccccaa tatggaacgg tcaggatcaa aaagggttct
    12001 catgacgtga actacgcgaa atgacggttg taacgacaat acaaaattaa tcgggaaggt
    12061 cacgtcacgg taactgcaac aaaagggtct ggcacaacga tgggcttcct caagaaattc
    12121 attggcagcg tgttgcgaga agggtctcag gcaccgtcgc ggagcagctt cgagcatctc
    12181 agtgaggaag agcttgaggc ccacttgaga attgccaaat atggaaactt taccctgact
    12241 gatgccgtac ggccctcgta tgatctccaa gtggttcctt gtgaagggtt ccgccacgac
    12301 gtttatcgca gtgacaacgg gcaaacggtt atcccggtct tgatggcctc tgtctccaag
    12361 gagaagctgt tcgatacctt tatcgagctg ttggatcccc ttggtgagga ggtcgatgta
    12421 gtgcttgaga ctagccatca ccgggatcgg ccgggacatc atgacctcta tcgggagcac
    12481 atcgacatgc ccgtcttgaa aagcatcctc tgggagttcg aagatgtcct tcttaatgat
    12541 ggctgcacgg gcattgccgt gttaaacgcc gatatcccat tggaggtcca atttgatgaa
    12601 cataagctgc ttatctgtta cggctcggat ttgcggccat ttgagagaat tctgaaaagt
    12661 cacggaatcc ccaacaatga gcaaattcgt ttcatcacgg aagccgaaca cgtccattcc
    12721 tcgacagagc attactcccg tcgctttgaa gaactgaaaa cctacctagg gatggatgaa
    12781 ggttgctacg tttgacagat cgaccagctg aatgatgtct attccgatct gacgggtagc
    12841 cggggactac ttcggcattg ccattccatc cgatactctt gggccttatc tgtgccgaaa
    12901 gtttgggtga gatgtcggag ctcgggcgca atcaactttt cacagccggg ttgccgaagt
    12961 tgcctataga gaagaaatgt tgcccttaac cattgggtag gatatgagtc ccgcacggcg
    13021 tcggctgaag ccccatgtcg cctctcccac cagggcaacc gctgcgtggg aggatagctt
    13081 tgttcattat ttgtcggccg agtgccatct ctcagagaac acagttgcgg cctatcgtcg
    13141 tgatcttcgc cgtttcctgg aatggcttaa cgcgcgagac ctcaagggcc tgactgtcaa
    13201 ggacttgtcg gattatgcga gttggctcca tgagaaggga ttggctgcca gctcgttagc
    13261 gcggcacatc gtatcactac gtctgttcct gcggtatctt caactggaag gcgttttaac
    13321 cacaaatcag gccgagctac tggcgagtcc gcaggtttgg gagcgaattc ctaaggtctt
    13381 aactcctcag caggtcgatg ccttgctcga tagtcccaag ccgttcgatc cctgttatcg
    13441 gcgtaaccgt gctatcttgg agtttttata cgccacggga tgtcgggtga gcgaggtggc
    13501 caatctgcgg atggaagacg ttcacgtcga ggaacgatat tgtctctgtc ggggcaaagg
    13561 taacaaggag cgagtcgttc ccctagggga gcgtgcgatc atgcgttttc aggagtacct
    13621 ggagcacgag cgaccgaagt tggcgaagaa aaacggcggg gacccgccct gggctttttt
    13681 gtcgtatcgc ggacgtcgga ttcggcggga aaggatttgg gaactactga agctttatgc
    13741 cctgagagcg gggcttcccg gcgacatcag tccgcatgtg ttgcgccata gctttgcaac
    13801 gcatcttctg gcgggtggcg ccgaccttcg gcttgtccaa gagatgctgg ggcacgcctc
    13861 catcgccaca acccaaatct atactcacgt agatttcgca cgactcaagg ctatccatcg
    13921 gcggtttcat ccgcgggcct gaaccgtcgc cggtggcgtg gacgagtgct tgagagcttg
    13981 tggggcaatg ctcacacaca atgtttaagc gcccatccca taatctcccc tcacgcgttc
    14041 agcgggagag ggggcagggg gtaagggtgg ctcgaatcgc tgccggtttt ctcgatagat
    14101 tccgacgtgc gcttgccctc accccaacct tctcccggag ggagaggggt ttttaggata
    14161 gactctaaat gccctctgac cggggtgggg caaatttgat cttctcgatg agccgcacga
    14221 tttgaccatc ggggctaaca tatcccgaga ccgctgtgat tgcccgaatc acgtactcat
    14281 accggaggtc gtagtcgcaa tccagttcga cttcggcatc gccagcactt cctgggccac
    14341 cgctatcgcc caccaaggcc cggatttgct cgcgtaattc gttaaaattt gcgagacgcc
    14401 gttcggagag gtaaagtccg gccaattcac ctccaggtcc ggccgtcatg cggactttga
    14461 tcggaggcaa ctgcaaatcc gacggaacac ccgcgctggg tgccgaaagc ggcatcttaa
    14521 cgaagaagtc tccttccggg gccacgattt taaacgtcat aatgaagaac gtgagaagct
    14581 ggaagacgac gtcgatcatc ggcgtcatca tcagctcgat tttgcccccc acgtgttttc
    14641 cagacccacg aaacttcatg ctatggactc tcctgttttg cccggagtgt gaatttttca
    14701 aacccggtac tttgacagat gcggattaac tcctgaaccc ggccggtttt ggcaaagcgg
    14761 tccgcgcgaa tgatgatagt ggtatttcga aagtcaccgc cgcgacggcg gatgagttct
    14821 ttttcgatct caagcacagg ttggacgttt tccaaggtta gctcatcgac gccgtaaaaa
    14881 acggtgccct ttgatgtcat ttgcaacgtg atgggccagg cgggagctgt gtccggcggt
    14941 ttggccagtt cactggcagg caggcggatc aactgattct gatcgccttc cgaaaagttg
    15001 atcagaatca tgaagaaggc gataagctga aacgtcatat caatcatggg cgtcatttca
    15061 gcttccgcct caggtatgtg ccgggcagca cgacggggca taatctgcac ctctgcgatt
    15121 tagaaaacaa cgccaaatgt tgtctgtcga tgatcaatgg cgttaatgtt tccgacggtc
    15181 atttcttcgg ggccacgctt gagaatcgtg acatcagacc ttcgctaacg ataccgacct
    15241 caagcacaag tcgggcaatc cgattccgga aaattccata aacgatgact gccggaatgg
    15301 cgatggttag acccactaag gtcgtgatca aggccgtcga aataccgcga gccagttcgg
    15361 aaggcttggg tgtcgtggta ctattggcga tcacactgaa tgatcgaacc attccatcca
    15421 cagttcccag aagtcctacc atggggctaa tcgtaccgat cagtcccacg tagctgagcc
    15481 ggtgttccat ccgcatggcc tgctcctcgc caacctcttg catggcttcg agggcctgcg
    15541 ggtaacctgc cgacagcttt gccaaacccg ccgacaaaac ctgtcctagg aaagactcgt
    15601 cattcttggc aagttcgtaa gcctcctgga aacgcttttc gttgaacagt gtttcgaatc
    15661 cctcgatgag tgcttggggc atgacggcct cccggcgaca agccacgagg ttcatgacca
    15721 aaagtgccac gaaggagaac gagatggtga ggaacgctag cgtataggtc caacccaatg
    15781 attcgaagaa ccatgcgagg acagattttt ccggggcggc aacttggcct tctgaagggg
    15841 cttctgccgc gggggctggt tcgtccagtt ggtcgtcggc tgcctcttcg gccgtcgctg
    15901 acgcctgaag ccagcccgga agctggggca caaccgctag cagaacgaac aggctcaaaa
    15961 atgacccgag aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacctccgc
    16081 ggcccgctcg ggatgaccga cttgaggcca caattcgagc aagttctgaa gtgcttcgat
    16141 atgcgatgga ctgtgccgga aataaatgag gtctgtgtgc aaaaaggcaa taatggcacg
    16201 ttttgcatcg ttggccttgc ggtaggccaa gcccaacgta acgtaagccc ggcccatcac
    16261 ctcggcgttt tccgtgaggt cagggcgctt caacaggtcc tggattaagg ccacggctgg
    16321 ttccgtttgt cccttttcag cgagacaccg cgccttaccg atggtggcaa ggacctttgg
    16381 agtatcgtcg ccggagagct gaatgaccag cacctcatcg aaggccttct cggcttcatc
    16441 aatcttgcct gctccgagat acgcccagcc gatggctgtt tgtgcctggg ccttgtattc
    16501 cggccagggg gcctgactga gactagcata gtactcaaca gccttgtcca ccgttccctt
    16561 ggcgaccagc aaatcggcca cgagccttgt ggcctcccag acatggtagt tgttcgggta
    16621 gttaagggca aagtcggcca cgagctttcc tgcctcgtca atcgatcccg taccacgaag
    16681 cgccaaataa gctgtggccg ctgctcgata gaactcgatg tcctggcgga tttccggtcg
    16741 ggtcacggtg tctagcttga tacgctcgag agcggtgatc acgtcttcat actggctaac
    16801 ttgcatcgcc atccggatgt tcttcagggt tgccggctcg ccatcccagg agatactttg
    16861 aatctggttg acagggattg tacgagggcc gctggtcgtc tcgatgacga cttctgtcct
    16921 ggtcatttgg gtgatttttc cagcgacaac ctggttccct tgttgaacca catctgccgc
    16981 ggaaacgatc agcgcgggaa atgttacagc gaccacgagg aacacagttt ttacaaaagc
    17041 ttgcatcaaa aactccttct tagcttattt cacaactcta agacaaccag gttggtacct
    17101 tcacctgatc atccggcttt tgacgatgct ggcgttgccc gcggccgact ctcaaaggcc
    17161 ttcaaacctt gcgggttctt ttcaccgcga agctgctgaa tcgttcgcag taacttatcg
    17221 tacttggcgt accactcttt tccaccgagg ctggggtaca gaaggtaggt cctttggatg
    17281 tcaagctcag cttccttaag gagttgatcg cgtttagcac cggttgcttt ttgtgccaat
    17341 ttgaatcggc aataagccag gttgtagcgg gcctggtgaa aaacgtccag aaagcgattg
    17401 tagtcgcttg cgacccgtcg cgcgatacca ctccaacccc aaatcaggta ccgaccgttc
    17461 ttctcgtgcc caccacggat ggcgtattcg tagtactgtt ctttccccgg ctgttccgcc
    17521 cattcctgat aaatttgagc ggcctccagt tgggcatcca aacgattctc tttgccctgt
    17581 ttgtcgataa cgaacagaat ggcttccatg gccttgtcga acagtcgcca tccccgtaat
    17641 gccgcagcca ggcgaacgcg gaggaagagc ggcgcatctt ctggcccaaa gctaggttct
    17701 gcttcacaac gtttgataag ctggatatat gtttcgacgg cctttttgta atactccttg
    17761 gcttgttctg gtacttgagg accaccagga tccaatcctt tcgccaggcc caaaaaggtc
    17821 tcggccaccc aattgagggc gcggaagtca acactcgcgg accgttcctg aattgcttcc
    17881 aaaaacttcg tgaagccctc cagcactttc tccatttctg cggtttttcc ttcggagcgg
    17941 agccgggcaa gctgctcttc caattgacgt cccagaccca catagacggc cgttagtcgt
    18001 ttggtcgcct cggcatctcc gctgcccgcg acgagctttt cgagctgctc ttgaacctca
    18061 aaggccttgt ccagattctg acttcccaca tacgcccgca gagccacctt catgacctct
    18121 tctcggagct tggcattttc cacagccgca tgtccctgcc ggatcaaagt cagagggcct
    18181 agagtcgcat cttccaacag ggtaatggcc tggtctgttt cacccgtatt cacaagcaac
    18241 tgggccaaga gcaacacgga atactcgacg aggtaatcaa cctcaccgcc tcccgaccgg
    18301 attttttgga gtccttgttc caggtagcgc cgtgcctcct ggagaatcgc gtcgctctcc
    18361 tccggactag ggcgcgcatc ttggctggcc gctaatttga catattgcgt ccatagcact
    18421 tggcccagtc gaagttcagc ccgcgcgcgg cccgaggatt ctaagggaat gcgctcgaga
    18481 aattccttag catcattcat taggttcagg tcgaaaaagc tttcgaggag cgtcaacaca
    18541 cattcgctcg cctctcgttc gttcccccat tgggagagtg cgtactccgt caattgcctc
    18601 attttctcca attcaaagga cccgtcttgc ccatctttcc gcgcttgagc aaaaagattg
    18661 cgataaacct tgacagcaat ctcgacggcc ttttgggatc ccgcattttg tggatagttg
    18721 cgggctaaga actcacttaa gacagccgac ttttcgaggg ctcctgcatc ccagaatagg
    18781 taaatgagct ggtacctgat agcgttcaat tcttcgatcg gctgatcctt atacaaagcg
    18841 atagcctttt ggtaaaagct aatggcttgg tctcttgccc gattggcctc ctgacgaatt
    18901 tgggtacgat cgccttcacc cttatccagt tgtgccatgg cggccagaaa acgtccccac
    18961 gccgcctcgc cgcgcgcttt gccatcggca aaatcgacga cgggggaatc ctcgatggtt
    19021 ctctcctcgc ccaataattc tcccagtttg gattgggcat ctaaccggta ctgtcccgga
    19081 acactggcaa cgatcttgag gaatcgctgg gccagtgccc gctgttgttg agctgttcgg
    19141 ccttccttgt tcttttcagc actcttggcg agttcaatgg cagaaagggc gccgtgatag
    19201 aggatctcta acccccaatc ggtgtttcgc acatccgggc ggaccgcttc ctcccacgac
    19261 accgcagcag aaacgatatc ttgatgcttg ttttgctttc gataaatctg gatcaaccgt
    19321 gccagggcct cggtgcggag ccgctgaatc tccggcaaat caccgggaac cgtgagggtt
    19381 tccttgaaga tgtcaatggc cacgttgtcc tgcccaagct cctgggcaca gataccctgg
    19441 taaaatcgag caaaaaaccc agccgagaag tcacgatatt tttcgtaaaa tttttcgtat
    19501 tcgcctgcgg cttgcttgag gagttccgca cgctgcttct cctccttacc gtacgtctgg
    19561 gccagttggt aaatagcccc aatcgtgaac atgcgggcct tgagatactc ggcgtaggcc
    19621 tgttcgcgtt ctcgggccaa ttgttcattc cgtgcgtcaa tgactttgcc ctggaattgc
    19681 ttggcccgtt cgtaagcact cttttcgtaa gcgtcgaaat gctgcttggc ttgctcgatc
    19741 agtttgcggg cctccccacg caaacgactg acttcgtccg gttttttcgt ctcaataccc
    19801 tgaaggaggg cctttgcctg ctccaccaat ccctggccca attgggcacg ggctgcgtcg
    19861 gccagtgcgt gctgaggatg ctcctggata aatttgcgga gccgctcaag cgactctttg
    19921 agggcttgat cccttttctg cggattgcgg gccgtttggg ccgccgctac caaagccacc
    19981 ccggcttcgt aatcaatgac ctccttcagc tcggcaggac acgtgggatc ggaagccaac
    20041 atatccaggt actcgactgc cgtgtcgtag tatccacgag atttaagggc ttccaannnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgag gtgtaccgga agtctgagcc
    20221 aggagagacg agtatgcaaa actgccaaca agcgccaaag caaccggcaa tcgcggccaa
    20281 atcatcgaac gtgttgcctc catccggaac cagcaaaggg ttgctcccct tttccgcaag
    20341 gggtgatgta ccgttaccta tcttttacaa actgtcagcc ctccagtcca gtagtctagg
    20401 ggggctatta cttccgcaca gtctaatctc tcatcgtcga tccacagcgt cgcgggccgt
    20461 atgctgaaac tcatccgtgg attcgaaaat atgttccgat cgtgcaataa aggctgattg
    20521 cagaggcgat tgggatttac gtttcccggg gagaatggta acctatatgg ccattttgtg
    20581 tggtcgagaa acgtcttgga taccgttagg aatccaggga tgcctatttt tacacggtat
    20641 gtaacctcgg aactcttgaa gattttcttg acagcgttgc tgattatgac gctgttactg
    20701 gcgttgggga ttggggccag ggaaggcatc agccggggat tgccgcccct cctcgtgatg
    20761 cggatgattc cctacctcct tccggagatt ctcgggatca cgattcccat tgctgtcctc
    20821 ttggctgtca gccaagtctt ggggcgaatg gccggaagca atgagatcat cgcgctacgg
    20881 gccgctggcg tgtcgccttc gcttgttgtc agaccgacgg tgatttttgc cttttttgtg
    20941 agtctattta cggtctggac ctatgatctg gcggccacgt ggggccgacc aggtgcccgg
    21001 cgggtcgttg tggaatccgt tgagcagatc gcttatggca tgcttcgaac gagaaagagt
    21061 tatagcagcc agaagttttc cctcgttgtc aaagacgtcg tgggcaaaag gctcatcagc
    21121 cccattatca ctgttcaggt tcagcctgaa agccctgcga tcacgctgac ggccgaggag
    21181 gcagaattgt ttacggatcg gaaggcgggt gccttgacaa ttgtttgccg aaagcctgtc
    21241 gcccgaattg aaggagaggc caccgttacc ctgccggaaa cctactggta cccgatcccc
    21301 tttgagacct cttacgagcc tctccatcgt gattggttgg caatgtcaga gattccggct
    21361 gctatccgca aattggaagg cgatgcacgg cagttgaaat tgcttctcac ggaggggggc
    21421 gcatcagagg agcagcgcag acgatggaat gaacaactcc agaacttgga attacagatc
    21481 cgcaagttgc agaccgaacc ctaccgaagg tgggccaacg ggtttagcag cctttgtttt
    21541 gtacttttgg gttgcccatt agcgatgtat cgtcgcagcg agaatttcct ggcgattttc
    21601 tttcttagct ttcttcccat cgtgattgtt tactaccccc ttttgatgtt ggccaacggc
    21661 ttaacaagct ctggaatcct gccggccgtt ggcttttgga ttgccaacct ggggatcgcg
    21721 atcccgggcg tcgtcctcct ttactactac tgcgaaacat agtcgagaaa agatcaatga
    21781 tcggctcggt cactgcaaac tgtgaccggt tatccgacga ggtgaaatac gacaagatca
    21841 acttgcccaa tttttccaat tagtctccca cggccaccgc aaacgcaacg gcatgtgtgc
    21901 gacaatggga gatcgaaatc tgaatctttt caatgcctag ttggtcggca attttcttag
    21961 ccccgtttct aaggatgacc tccggttgcc ctcccggacg attgacgatt tcgacatcga
    22021 gccaactaat gcctcgaatc cagcccgtcc caagggcctt gaggatggcc tctttggcgg
    22081 cccatcggcc cgtaaaatgc tgcgttgcct gttttcggtt ttggcaataa gcgatttccg
    22141 cgggagtgta caccctctgt agaaagatct ccccgtggcg atcaatcatg cgagcaattc
    22201 gcagacattc ggtaatatcg gtcccaattc caaggatccg catgggcttg accctgtcgg
    22261 ttgagcaatt tcagtagcac cgtgcaagcg ggattttact ttgctccgga cggaccaaat
    22321 caaatggcct tctgatttgc tacggcgaaa tgtcgccaaa tcaggggcat gtcttgtaag
    22381 agtcagtatt taatcccaca ggcattctgt gcccgttgta ggacttctgc ggccttttta
    22441 cgggccatgg ctgcgccgtc gcccagaatc tcgcgaacga actgcggttt actttccaac
    22501 tctttgcggt attctctggc ctcggcgaaa tacttttcgg cgacatcggc gagggcctgt
    22561 ttgacctctc cgtagccaaa tccgcctcga cgataacgag cggccatttc ctcgcgttcc
    22621 ctttctccga caaacagaga gtaaagctga aacagatgat cagtttctgg ggctttgggt
    22681 tgctcgatag gccgcgaatc ggttacgatc cgcatgatgg tcttgcggac cgtcttcgga
    22741 tcgtcgaaga tgccgatcgt gttgttgtag cttttcgaca tcttttcccc atccgtgccc
    22801 gggacccgcg cggcattttc catgatcttg gccttcggca tcacgaagac gttgccatag
    22861 agatgattga aactggaggc gacgtcgcgg catacctcaa tatgttggac ttggtcctcg
    22921 cccacgggca ccgtatccgc atcgtaggcc aggatgtcgg ctgccatgag gacagggtag
    22981 gtgaaaagcc cggcgtctgc cggcagacct ttagcccgtt tgtctttgta agcgtgacag
    23041 cgttccaaaa gccccagtgg ggtgcacgtc atcaggagcc agcaaagttg ggtgacttcc
    23101 gggacatcgg actgaatgaa gagtgtggcc cggtccggat tgatacccaa tgccaggagg
    23161 tcgatggcgg cgtcaatcgt gttttgtcgc aaaacctgcg gatcacggac cgtggtgaga
    23221 gcatgcagat tggcgatgaa ataatacgcc gcatcggcct gatcctgaag ctcaacatac
    23281 tgcttaatgg caccgaagta attaccccaa tgaaaccgac ccgtcggctg aattcctgag
    23341 acaacgatca taaaactacc cgattattaa acgaacctca ccaaaccaca gattttcaca
    23401 acggatggcg atactgtatt ccagactcct ctcagcactc acgtgttttt ctaaggtcac
    23461 acgctttcct gggttcgcaa agtgtcttca caatctcccg aatcgaatag gcccccaact
    23521 cacgcaaggc agagggcaaa gcgtcgataa tttcccaggc ggccatggga tggaagaacg
    23581 atgccgttcc cacctgaatg gccgaggcgc ctaccacgaa aaactccatg gcatcatcga
    23641 cgtttgtcac gccgcccgcc gcaatcaccg gaatatccac ggcatgcgcg atttggtaaa
    23701 cacaccgaag tgcaacgggc ttgatggccg ggccacttaa tcccccaaaa aagccgccca
    23761 ggcgagagcg gcgcctcctc cagtccaccg ccaggcccaa caaggtatta accgccgtaa
    23821 tggcatcggc accagcgtcc gccgcagcgc gtgcaatcgt cgcaatatcg cctgtgttgg
    23881 gggtcagttt tgccaagatg gcccgactaa aacgctgcct gacccttgcc acaagcacat
    23941 ggcactttcg cggatcggat gcaaaatcga tgccgccggc tacgtttgga cacgaaaggt
    24001 ttaattccac tgccgacaca ccttgcgcgg catcgagaat ctctgccatt tttacaaatt
    24061 cgtcctcgtc tttggctgcg atactaacaa tcaccggtgg cccgatagtg gacaatgcag
    24121 gcaaccgccg ctccagaaag gccatcaaac cgtcgttatc gagcccaatc gaattgagca
    24181 tcccagccgg tgtttccacc gttcgtgggg gcggatttcc tattcgcgga gagaaagtca
    24241 cggtcttggg gacgatcgcc ccaagccgag ataggttcgc gatcgggtaa ccatcgacga
    24301 tgtttccaaa agtacccgag gcgaacataa caggattggg tagcacaagc cccccaatgc
    24361 gaatggatag atcacattcc ttgtttgctt ctatcacgga aaaatccgaa gaaaacgctg
    24421 gtcaagttga aaccggttgg atgcgcgtca aaaccggcca tttactctaa tctagccgtc
    24481 ttgttgactt acgacaacgg aaacccaacc ttgccccctg gctgacccga tcttccccac
    24541 tgaggctttc cgcttgccaa gaacgtcaaa cctagcccat gcgaaggccg gctatcactt
    24601 cttcagcttg gccatcgaca ccagatgcgg gatctgccac aaggctacct tttgcaagct
    24661 atttacatcg gtggcaggtc acggccagaa tatagctggc ctgcgaggtt agcggcgttg
    24721 tcacgtaccg accgaaaagt tgccgtgcga ttcacggtac cgccagagga ggggaagaga
    24781 caatgaactt cgagcacaaa aacgattttc ccttttgttg tggatgccga tatcctcata
    24841 gcaagcaaag cgtggaggat cggcaaacca cgggcgtgac gcgtcgggac tttttgggcg
    24901 cggccgccgg cggtgttgtg gcctcagccg ttttggcaac tacaacccac ggtgcctggg
    24961 aaacggcaag ccaccggcct ccctctgtca agacactcca agttcagccc gtcttcaatt
    25021 gtcacattta cccacgtcag gaagccacga gctggcgcgt aacgggtgcc attcaggatg
    25081 aagcggaact tcgggaagaa gagaatcgaa tccgagccga tctcaagaag ctgcaagaga
    25141 tggctgactt tcccatggag attcggcccc ttgtcaccgt gggaactgtg gaagaagcca
    25201 atgccgtggc atcacagccg tgcgatgtga tgttagtctt cgcggctcgg cgcaatgtgc
    25261 cggttcttga ggcactgatt cgccctgagc ggtggaacct gatgtttgtg cgtcaccgca
    25321 gtgggccact ctattacatg tacatcggag ttcatgtcca ctatctgcgg aaacgccgag
    25381 atttcttcgc cgaaccggca atgaccatcg atgacgtggt ggtggatgaa ttacccgaac
    25441 tcctttggcg acttcgcgcc ctatttgcgg ttaagaacac cctggggcag cggattgtgg
    25501 ctatcggcaa cccggccggt tggggtgccg acggtgcgca ggcccccgca cgggcaaaag
    25561 aggtctggaa attcgagtat attacagttc cctatcctga gctggaggag cgtctcaaac
    25621 aggcacgggc gaacgagcgt ctggtgcaac actgtcaaca gcaggcagag gcctatctcc
    25681 gtgacgccca ggtttcgttg gaaaccgaga gggggtttgt ggaacgggca tttgtactga
    25741 ccgaggtctt tcgcacgctt ctcgatgagg ctgacgccaa tgcaatcacc gtccacaatt
    25801 gcatgacgac catcatgccg gtttcccgca ctacggcttg tttgccgtta acactcctca
    25861 acgatgatgg gtatttggcg ttctgcgaat ctgacttggt ggccatcccg gcagggattc
    25921 tgctccatta cgtgacgggg aggcctgtat tcttctgcaa tccgtcgttt ccacacaagg
    25981 ggatcgtcac ggtttctcac tgcacagccc cccgccgtat ggacgggaaa tccctggccc
    26041 cggcacggat tctcacccat tatgaatcgg actacggggc cgcacccaag gtggaaatgc
    26101 cccgtggaca agaggtgacc gtcattgatc ccgacttcaa tggccagcgg tggctggggt
    26161 tcgaagctgc cattatggat agcccattct ttcccatttg ccgaactcag ctggatttga
    26221 agatcatagg ggattggcaa cgactaatcc aggaaattcg agggtttcac tggctcctgg
    26281 cctatggtcg cgtgctaaag accttcggct atgccgtccg caaggcaggt ctggactggc
    26341 taccaataga ggcttaggtt ctatccgaaa aagccccctc tccccccggg agagggtcgg
    26401 ggtgagggca agcgatcgtc ggagtcgagg gagaaaaaag caattcgagc cacttcaccc
    26461 caggtccctg tcgcggcgaa cgggcgaggg gagattatgg gataggctct tagtcctgtc
    26521 aggggtggga ggcaaccgcg taacactttc tgtagcgtgt attgtggttt tttagtcggc
    26581 acgctcccaa ggcggtggga gctcttttta tcgggttagg gctggttcct actattctgc
    26641 ggtgggcaaa ctttcggcga cgatgcggaa tcccacgtta aacacccggg cgtaaggcgg
    26701 ataggctaag cggaaggatg acgttgcacg ctttgggcga tcgtaccagg aacctccacg
    26761 aaccacccgc agtccggatg ccgccatgtc attgcggcca tcgttgtcgg catatggata
    26821 gtctcggtaa agggaaaggg tccattccca gacattaccg tgcatgtcgt aaagtcccca
    26881 cggattgcta cgataagagc cgaccgacac cgaaacaagg ccgccgtcat tccaacgggg
    26941 gtctttgaga atccagtcgt caaaatggtt aggattggcg attgtcctga cctgaatata
    27001 cgtatccatg acaaattcgc tcagtctttg gtctgccaag ttggcgtaag gggagaaatc
    27061 atcatccagt gaaccgaacc aaaacggctc ggccgtcccc gctcgacagg catattccca
    27121 ttccgcttcg gtgggaagcc ggaagcgtac tccttgccgg ttagaaagcc aatcacaaaa
    27181 ggcaacagct tcgtgccaag tgacgcggac cacgggctga tctggctggt taagtgggaa
    27241 tccgtggtaa ccaaactgat aaccatgaag ggattcgaca tggctatcat gcgtcggatc
    27301 gaacacggca aactgccggt ttgtgatctc gcatgtgctg atccaaaagt cgtggggaat
    27361 ctccacaaca cacggcggcc tttcatcgat ctcaccttcg gcattgccca tgacaaaccg
    27421 cccggaaggg atccgaacca tggtcaccgt cacaccgggc ccgattgtga tgaccctttg
    27481 ccaatctttc agcttctctt gtcgttccct ggccacctca tatgagaaag gccagtcatc
    27541 gagtttgggt accgtgacgt cttgcggcgg tttttcgccc gaagaccgct cctccgttgt
    27601 gaaactgaca agcttgttac catcgtttct ggtaatttca tcaggagttg tgccgattgt
    27661 tgtcccaaaa acggtgatcg tcggtatttc ttcccaatta tcatccattc cggtgtagcg
    27721 cttgcgcatt tcccgggcgc gggcaagtaa tggcatcact gtctctggac cgacaatctc
    27781 cgaccatgtg ccatgataag gcgcattaag atcaatccag gtgtaaaacc ggtcccacgc
    27841 ctccggcggc aagttaaccc cgtagtggcc ctttttcaaa acttggacca gttccgttgt
    27901 ggcggcgtga tattccatgg gtgaaaggag atggatgtca ctctcgatac ccggacggcg
    27961 gacaaatgga tggaggttcg cgtaagcaac ggaaaattta cctccgactt tgtccgagac
    28021 atggccggcg atagccgacg accagccctc cagcggcacg tttcccctaa gatcaaaggc
    28081 acctggtgac tcggactgac cattgtggca ggagacgcag taccgatcca gaaccggttg
    28141 gacttcccgg gcgaaactga acccacgcgg tggcccatac cacggttcga tcttagcagg
    28201 ggggtgggag acggctagac tttgcaggtt cggcactgca tcgttgggat gttcatggca
    28261 accgatgcag gagaggacct cgccgggcat cgccgtgaac caactccgca tgatttgcaa
    28321 ggcacggcct tcggcatcca aaggctggac ggcaatcggt gtatatgcgg ggacccggaa
    28381 gaatgcggag ccatcctctt gtactggaac ggtccccaac acacgtttaa tatcccatgg
    28441 gccatccata cctatcgcgc ccagcagtcc ccccatacct cggaagctga actcgtaact
    28501 gactagccga agttttttga cggtgccgcg agggataccc ttcaatccgg gaccgcggta
    28561 cacatccacc aaatagacaa gggcctcctt ttgtaaagga tcagttcggt cggggatgac
    28621 gggaggtcgg ggccgagggg caagagggat tggctctagc aaagcgaacc ccggcttttc
    28681 gcaaatgaga accaagttcc caaagatatc agccagatag attccccagg attgttccgg
    28741 tgacggcttg cagctcacca ggaagtactt ctcgcttaat ggccaaggcg tcaaaaactg
    28801 cggccaaacg ccatcaacca gttcatcccg gatggtaggc tcgatcgttt gatgagtggg
    28861 cggaatctcc tgaacaaccc cttgtgcctc atgacgtccc actgcgggat caacaatgag
    28921 caggcggccc gaccgccgtg ttccgtgatg gccactggca atgcccacaa ctttcgtggg
    28981 gtgcccaggg atcgctcggg cataaaagaa cgaattcgga aaatagctat tgcttccgta
    29041 gtaggccatt tgatttgtgc cgtcaggatt cattgagaaa agaattcgtg aatgcgcatg
    29101 gggaaggtcg gtgtattccc accgcagata aagaacacgt ccgttgttga gaacacttgg
    29161 gtgccagttg tgttcctgat cgaccgtcag ttgtcggatg tgaccatctt tcgggtccca
    29221 acggtaggtg ttggtcacgt gcgattgtcc atacacacag gggaccccaa caaatggcgc
    29281 tgtggaagtg aaaacaacgc gatcatctgg caagtaacag gcgtcatagt tgtcgatgtc
    29341 gggttccagg ataagcggca attcgcgtag gccggtgacg cgccaccgtc cctcctggtg
    29401 ttcgagatca agctcaaaga cctggaaccg gttgaatgat cccggcattg agacgaggag
    29461 tcttttggcg tcaaaatgca ggtcaatctc ccccacatag cgaccttcgg ctggttcaaa
    29521 gaggacctgt ggaggttcat tcctccgacg cagggaaatc acgcatatcc tgttgtcata
    29581 gcccgtcggg ggaagactcg agtggctttc ccagttggct ggtaagccca atttgggttc
    29641 ctttctctcg acataaagca gtgtatcaaa gtccaaaagg ggattagcga gcaaagcctc
    29701 ttgttgaagc tcaagaaacg acgttctgac ttgctggatt gcttgttcat caacagaatt
    29761 gcgacgccgt aaatcatcac aagccgtttt cagtttttcc cacctgtctt gaaaaagatg
    29821 gccttcccgg tagcgatcgc caaatgaggt gatcagatcg tcgatggccg cttttaaggc
    29881 tgccaactcg cttgtgctgg gaatttccgc cttcgccata gatatcgccg atgtctcgga
    29941 ctcttgccag tccacccatt ttgtatcgtc cgccagataa tcgagaatat tggcggtgag
    30001 tcgatctaac gctcggcgat gaacatcatt ggctagggca taattgggga ttcgccagcc
    30061 caggatgatg acgcggcctt ttccaagccg gtactcgacc agagggttct cgtcggcata
    30121 gttcgcacga gccaagagtc gccccgtttt tgggcctccg ccccggtaaa aatcggcgaa
    30181 cgcgggatat cccccggacg tgaaaaagat ggggctccct gggcgagcag tgagacctgc
    30241 gaagataggg tgatctctca ccacggggat aagcgcactt tgaaagccat cgcgcccagg
    30301 tgaagccgtt cgcatgacgc cggtttctag tcccatatca ataaccaacc gtgcggccgc
    30361 tcccgagaga aataacctcc caccttggct gacgaagttg cggagggcct ccagcgtgcc
    30421 gggattctgg agatcagccg agatcgtggg caaatcccct tggtgacacc aaacgatctg
    30481 gaactcggtc aatggtacgg gttctcctcg atcgttgacg aactgctctg gaccctcgag
    30541 ataaatcaca cggcagtcgc ggttagcctg gagccaggcg agagccgctt gggcttcttg
    30601 acccagcggc gttgtccgtg cggcggggat aagaaatgcc acagagccgg cgaatccggt
    30661 ttggctaaca gagaagatga cacccaccag cgcgacaaaa attgacacac atttaatcat
    30721 ccgcagacct cacatgccat cgggaatcct gaagatcggc gctactgcgc agcgactcgc
    30781 aattatccaa cgctcagtca aatccaagag ccaattttat taaaagagga ggccgaaagc
    30841 gacagttgag cgaggaaaca atgactagaa agagcatgaa cgagtataag tgtatcgttg
    30901 gggacaaaat aaaatcagcc ctgccggttg acgaccggca gggctgtgcg tgtcactgta
    30961 ccttacacca aacttgggca gaggcacggc actcaaatgg cttcctgcct tcacccagtt
    31021 gatgctgttg cttttgtggc aatcagacag agtatcagat gataccaccg tgtgttcggt
    31081 acggggtcat gtgatcagga tgatccagca tccaggcgcg ttcccattcg tcaagcataa
    31141 gccgtagtcc ctcggacgtg tagatcaatt gttggacacg gcggtcaggg tcggctgagt
    31201 agatgggaac aacacaaccc acgacactgc tgagcagaaa cgcaccgagg attgccacaa
    31261 tcagttttcg cattttgcca ctccgtcagc ataagttgac gagccaacct tgctcgtcat
    31321 ccgcgaacgt tctctccatg taaccggaaa cacacgccct gttgtttcgt gtatttctga
    31381 ggactcccag gggcttcggg gcctcattga tctcgcggta attcgaatgt cccgcccatt
    31441 ggaattcgcc gcggttggtt agggtcgcgg cttcttggac atggccttaa gccttttctt
    31501 tagttccttt cccggcttga atcgcactac atacttctct ggtacctgaa gcgattcttt
    31561 agtcctgggg ttgattgccc gacggggtct tcgtgtcttg atccggaaca cgccaaaatt
    31621 ccggagctca atccttttct cggtggtcaa tgtgtcgata atggcgtcaa atgttctctg
    31681 aacaatttct cgcgtcaggg tctgtgaaac acccagttct tcggcgatac gtcgtacaat
    31741 gtcctttttg gtcacgtgaa ttacctccgg cgaacccgac gagttttctc gatttcaact
    31801 accgatcgtt gtaaatctag ttcctgacga aactaaaatc aattacccgt cgcgccgtag
    31861 ggttctggtt cgctgaccac aagaaacctg gctttctgtg gccgattggg gacaactctt
    31921 aagtgacgcc accctgtctc atcggggtgt tccgtcttat ttgaccccga ccgcggtaca
    31981 acacggtcgt cacaggtctc acatccaata tcggccgaaa aatccgaaaa ctccaacttc
    32041 ccttgtgatt tctccgccac agagatgaca ggataggcaa agtgcgcaaa atgccagtgg
    32101 ggctgccttt ttctatgccc gaggcaacta ggctcgatcg gtttcgaaat tggtttcaga
    32161 tgtcaatcat ttgtttccgc cggtcgatct cctctagcga gtgcagtggc ccttggccgc
    32221 agcctgggca ggtccggcag atttcctccc aagtgcgctt tgtcttcaga atggatggcc
    32281 aaaaggggaa actccggcat tgtcgtgggc ggagttcata gaccaagcat tttcgggtca
    32341 gggggtggag aaagacgcaa tcaccgttgg gctgttcgat gagacttttc gctttcccag
    32401 tggtgcggac aaaccgctct tcgaattccg ccggggagag tctgaagaac tccgcaaggg
    32461 ccttgatctc ttcttggttc acatagacat atcccggctg accggaacaa cagtacccac
    32521 aaccggtaca ttcaaaccgc aggccttcat ggtaccaggg caaggctgtt tttttgctga
    32581 cacgttgcat agtcaaagat ttttctaagc gggaactacc ttggacttag gacaacacaa
    32641 atgatgaccc cggtcgcaac aatcgctgta acccacccgg ccacaaaccg taggccgccg
    32701 ccaagacctt aatggggtga agcgtcggct tggttgtgcc ctgctcaagc tgaatccgac
    32761 atgcgctgca ttccgtgctg gcggcctgga tggcgggatc gcggaggtga ctgatgatcg
    32821 accagccaag tcgcaggctg gtgcgaaagt ttttcgcctt gattccaaac atcccagcca
    32881 tccctgagca cgtgttggga agtccctcga cggtcacgcc ggggatcaac tcgagcaatt
    32941 cacgtcctgg ttggcccacc ccaagggctc gcaaacgaca tggcaagtgg tagccaagcc
    33001 gaaggggtag attctgaaaa tccgtccgca gttgtccatc acgatgaaga cccagcagga
    33061 attctccggc atcgtgggtg tgcttggcaa ccagttggga gtcctcgtct tggagaagct
    33121 ggggatactc gtgaatcagc gtcagggcgg cggccggttc cgtcgttaca atttcacatc
    33181 cctgccgtac ggcctcggcg aggattgcca cgttcagccg cgcgtgccgt cgcgccagat
    33241 tcatttcgcc ggaggcaatc gcggccgttc ctgccgacct ctgctgtagt gggacgaaga
    33301 cggaaatccc atgatgggcc aggattgcaa caagggcctc ggcaagtgct ggatcaaacc
    33361 agttcgcata aacatccaca aagtaaacga ccttccttcc gggaagccgg tcaaggcggt
    33421 gcagtttttt gcgagcagcg cggtggagaa aactgacagc atgtagccgc ggtaactttc
    33481 ggacttggga aataccggtc aacttctcga gcagccagcg gacttgccgg ttcgccagac
    33541 cccaattgac aagcggggca aatcgggaga gcagggaggc caatccgtca atccgattca
    33601 gcagccaatc agcgaaggcg cttccgtggg ccagcgtgta tgccgccttg gcttcactgg
    33661 ccagcagagg aatatccacc tgggcagagc actcaattcg gcacgaatgg cagtgcacac
    33721 agagatcaca aatctcgcga aaatcctgca acgttaatga ctggagggga agagtctcag
    33781 tgagtactgc ccgcatcaga ctggactttg atcgtggggc acactcttcc caaggcaatg
    33841 cccgaaacaa gggacacatc cttaccctgt ggtcctgcgt ccggcaatcg ccacagcccg
    33901 agcaccgcga cacggcgttt gcaaccaagt ctggttgcca atccaattgc gattccaaaa
    33961 tcctgggcaa attctctgaa cggtcatcca gtcgccctgg gatgtgatcc tgttgtgatg
    34021 tcggcggcgc atttagagtt agctcggacg gggggctata ggaataatgt tgagagtcta
    34081 tctcggccgc ggcacgatcc ccaaagtcta ttacgccagc tgttgatgcg gccggcgcag
    34141 ggatatcatc ttgtcgctca ccacgggatt gcgtttctgg gagagatgtc gcaccttggg
    34201 tcgtgtgaac tccggtccgc tggaagcccg cgccatcttc attgccgtcg tcttccgaac
    34261 taggagcctc aaacccaaca aacaaacggc ctcttgccaa tttccaaatg tcgccggtat
    34321 caggggtctc aatgattttt cctggattaa gaatgttaag cgggtcgaag gcctttttga
    34381 ttttctcgaa aatgggataa agatgccccc actgttgctt catgtagggt gttcgagcta
    34441 agccgcaccc aagagtcggg ccaatcccgc ctccgacact caaaacgtaa ccatagactt
    34501 cgtccgccac ctgtttcatt ttccggaaga ggtgacggtc ttgtgtatca aaaaatggct
    34561 ggaggtagat gacgcc
//
LOCUS       DAOSUT010000020        34435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_878_length_34435_cov_9.609395, whole genome shotgun sequence.
ACCESSION   DAOSUT010000020 DAOSUT010000000
VERSION     DAOSUT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 34435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34435
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_34435_cov_9.609395"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLD05_03085"
     CDS             <1..310
                     /locus_tag="PLD05_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76444.1"
                     /translation="ETWLLTDGLVTLEEMKALMPFVTCGGSVFKAQVVGYFAGGGPSC
                     RVEAIFDATVRPARLLFWRDMSHLGRGFQVDVLGTPSSSYGGAVIPGGSESGATLPGS
                     "
     gene            317..1879
                     /locus_tag="PLD05_03090"
     CDS             317..1879
                     /locus_tag="PLD05_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76445.1"
                     /translation="MAYWLAIEWNNEELRAVAAVRRRDRLVIEHAFAVPLTDAVRPSD
                     NGSPESREQTAENIGKRLAAALAARGIRRPTTFVAISRSLVELRQMSVPPVSDEELPD
                     LVRFQALREFTHVNEDWLIDYLLLDEDPSQPRNALVAALAPELFQDIQKTCQIAKVQP
                     ERIVLRPCATASLANRCVPPKPDEIRLLVDLLGEEVDLSVVVGNKLVFMRTARVTGDP
                     LKEADALVVLVGEIRRTMMAAQNQLGGGRANHLVLCGQTPAYQALGQTLRERLDIETT
                     CFDPFSHFDLGPEVAESLPDHPGRFTPLLGVLADEQEKVAPAVDFLHPHRRPEPPSQR
                     NRYALAGLGGLLIVVLAGMYGWLQLQELDSRIAGLRNRQAVLDRQLKQAAETRKLADA
                     IQAWSGGHVNWLEELRRLNEKIPPAEEVMLGKLTMASGARGGEISLEGFAARSESIEK
                     MEQALDDQQHRVISKGKAEDDSQKPYKLRFGTTVVIVPPSSASTSRPSSQRPATGPAA
                     SRTTPNAPLAGR"
     gene            1884..2840
                     /locus_tag="PLD05_03095"
     CDS             1884..2840
                     /locus_tag="PLD05_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76446.1"
                     /translation="MKLGRREQIMILIVGTVAAIVVGQLIWRLVATPFTARQKALATL
                     TSNVHTRERQLKELEKAKQKLEEWRARSLPADPVLARRLYQNWLLECSQKAQLENIQV
                     DSGEVRRERDLFYRLPFTVRAQGSLESLTRFLHSFYSRNYLHAIQRIAVTPLKDGKKL
                     DLLFTIEAAALDDAVAKDSLPTGELVEAKLDDVKEYTKTIVGRNVFVAYTPPPPREPP
                     RTPPRTPPRTPPPEPPPLDPIQFVYVNGIVEVNGQREVWLYSRTEDKTFKLTENQTFQ
                     VGKTQGRVLRIGIRDVEVEINGQTRTLTLGDNLLGKPISATG"
     gene            3264..5399
                     /locus_tag="PLD05_03100"
     CDS             3264..5399
                     /locus_tag="PLD05_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOP76447.1"
                     /translation="MPNRLIHEKSPYLRQHANNPVDWYPWGEEAFRRAKELDRPIFLS
                     IGYSACHWCHVMEKESFADPEVAAVLNQHFVSIKVDREEHPAVDAQYMEAVQIITGQG
                     GWPLSVFLTPDLQPFYGGTYWPREARFGMPGFLDVLRAIADAWEKRRHEVVAEADKVT
                     VLLSDISNTLPGRHLVSEEHVLQKAEQVLAEEFDPRWGGFGVAPKFPHPSVIRYLLTR
                     FAFQDSQMALTMGLRTLDAMADGGMYDHVGGGFHRYSTDAMWMVPHFEKMLYDNALLA
                     RAYLDAWRLTRRERYGQIVNETLDYLLRDMHHPDGGFYASQDADSEGHEGQFYLWTYE
                     EILNVLGPAAERFAEFYDITREGSFEGKNIPNRIRSANRWTALSVGERAQLEQEMAEL
                     RQRLRETRQMRTPPDHDDKIVLAWNAFAAEVFLLAGRVFDEERYLTAGTMALEFLLSH
                     LRDERGMFCHSWCQGVRGTPAVLEDEAALAVALITGYETMARRDWLEIAVTLVEDMRA
                     RFHDAATGVFFMTPADHPHLLCRRLDYFDNPTPSGTALAAEAMLRLGHLGGEPSYLAI
                     AEGALRSLAGWMQKSPIAVGHALFLRQTMVRPWTSWMFVLPSGEVGAVHEGKVPPPVS
                     RWLSSLMSGYNPSMIVSINWLGDGSPSSATDGLPGTKSLPDRPAVNGEPTLYICQGAS
                     CGAPIVGEQLIEKVVQDHVSDALTARNSD"
     assembly_gap    5615..5714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5715..6713
                     /locus_tag="PLD05_03105"
     CDS             <5715..6713
                     /locus_tag="PLD05_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76448.1"
                     /translation="QVITSQFTDLAATPGIGKKKLQGLLMLLERAAHTDPTSLPAETT
                     DQTSVAEGREKSPSEYLIRWQDVSELMWAEWRATVRKHGLHDIPLGRLAPALNRMTRV
                     LWNIPLGEFLDMTIEDLREERSYGERRLSALLEVFGLLHQILKNVEPQSYLAFELAPR
                     RIAAMQQWILQTWQSGKVPTEEEIKERFILPLLEQTRLDASEQTVMMVEQRLGINGPP
                     VSVRQLGRIFNLTRARIYQLFDELAEIMRVRWPLGRAYTQLLQSFIVYEYNRRGTGPD
                     ISQLTMAIEIYFPKPGERRQIVAAKGGLPDLSPGFATTMAETSPTAHIPEEMDEDW"
     gene            7107..8105
                     /locus_tag="PLD05_03110"
     CDS             7107..8105
                     /locus_tag="PLD05_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOP76449.1"
                     /translation="MADPGQRGFSPVQRILERYLPVWLVVLCTVAYFWPRLSGVPGDP
                     FLASKGYLKHMIAVTMFFIGSLLRPEELLDVFHRWPLVLFGTLVQYSSMPALAYLFGR
                     LMGLDGPFLIGVLMVGCVPGAMASNVLTLVARGNVSYSVSLTTSATLLSPLVVPFVMW
                     LTVGQFAEFPVVGTGIELLFMVVLPVGLGFTLSQLSRPWHKVGQRLGSIVANLVILWI
                     IAVVVAANRDTLQQLTGPLVGALLGINVGGYLAGFLAGWLVRLPVPMRRALTLEIGMQ
                     NAGLGTTLVLALFPDQPQAAVPCALYTFGCMFTGTLLARTMAMFPAPGQGSQPEAA"
     gene            complement(8123..9376)
                     /locus_tag="PLD05_03115"
     CDS             complement(8123..9376)
                     /locus_tag="PLD05_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76450.1"
                     /translation="MDARFRPVAAGLIVLLLTSGASYPTPNFVIHCGDPQLAKELGDA
                     AENYRKELARLWLGKTLPDWSRPCPVTVHVGPNLGAGGATTFVFDRGEVYGWQMTIQG
                     SRERLLDSVLPHEITHMILASHFRQPLPRWADEGAATTVEHISEREKHRRMLLEFLQT
                     RRGISFRRMFAMEEYPRDIMPLYAQGYTLAEFLIQQGGHRRFIQFLETGLKDNQWEAA
                     LSQWYPYKDLSDLQTSWVTWVAQGYPQLRPLPNPMDSSPQQPVLASLENQWRPPQSSS
                     TSAESVYQRSATQAAWNASASERTGVIAASYDGGARASQGRSDATQAIYSGGVELDVN
                     GWRPAPLPEAMEGTLGPMAMASSPQTSAVPSSPGVTAPCSCGQTVPTVDTPANAVQFN
                     QVAHPRTAEPSSRILLDWNRDTPWR"
     gene            9708..9953
                     /locus_tag="PLD05_03120"
     CDS             9708..9953
                     /locus_tag="PLD05_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76451.1"
                     /translation="MLRPQLRVYYGPQEDEQLEQSPHSGVTSVVNVPLREVLPLLVDA
                     VRSKRTWLKDFENEEITLSLDLYEVLLAYQHYRRPSA"
     gene            10532..11308
                     /gene="tpiA"
                     /locus_tag="PLD05_03125"
     CDS             10532..11308
                     /gene="tpiA"
                     /locus_tag="PLD05_03125"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOP76452.1"
                     /translation="MRRPFVAGNWKMNMERASAVALARAVADGARAYPQVDIAVCPPF
                     VYLEAVAQAIAGSPVALGAQNLYPEPKGAFTGEISGPMLKDMGCTFVILGHSERRAMG
                     ESSEFVNEKVFAALECGLKPIICVGETLRQRKEGRTLTVVRRQIDGSLSGLSAEQMRQ
                     CVIAYEPVWAIGTGENATPDQAEEVQAEIRRLLAERFGKDVADSVRIQYGGSVKASVA
                     ADIFEKPNVDGGLIGGASLKADEFLGIVAAAQKVKGRNGG"
     gene            11333..11746
                     /gene="secG"
                     /locus_tag="PLD05_03130"
     CDS             11333..11746
                     /gene="secG"
                     /locus_tag="PLD05_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOP76453.1"
                     /translation="MKFVFLILLVVTSLFLILLVLIQRGRGGGLAGAFGGFGGQSAFG
                     AKAGDTFTRITIGVAAFWIGLCIFTLLYFRWTHRSRLDLGPTGPSVTNTAPLAPTTLP
                     PAPGGEGSGASGGSSGTTPSGGTGQTGSGTQESGS"
     gene            11888..12781
                     /locus_tag="PLD05_03135"
     CDS             11888..12781
                     /locus_tag="PLD05_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOP76454.1"
                     /translation="MLLSMTGFGEARREHEGFSVRVEIRTVNARYLKISIRSNNAIPG
                     GLENEVEALIRRAIRRGTVNVGVFVDHEPQAGDYRINGQVLLGYLKQVSNLAVDHGLV
                     PPTTLDSFLLLPGVVSDLSDPAAQSERIRPVVIAAVEEALDRLQAMRRAEGHAMTEDL
                     RANCQRILELVERVEARAPQVVEDYRQRLQERMNQLLGPAAASLRPEDILKEASFFAE
                     RSDISEELVRLRSHVQQFQEILTKGGGEAPGRRLEFLTQEMVREVNTMGAKANDVEIS
                     HHVVEMKTLVERIREMVQNVE"
     gene            12794..13396
                     /gene="gmk"
                     /locus_tag="PLD05_03140"
     CDS             12794..13396
                     /gene="gmk"
                     /locus_tag="PLD05_03140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOP76455.1"
                     /translation="MSGQLPGRLVILSGPSGVGKTTVANRVLAESPVPMVRSVSVTTR
                     PRRPDEIDGVHYRFVSPEEFERLRAQQAFLECFQVFGNGHWYGTLRQAVLSALSAGKW
                     VLLVIDVNGALAVQEEFPDAVSIFLLPKDWQSLEERLRGRGTEPEEVVRLRLERAAEE
                     LRLAEKYRYRVVNDDLEQTVRDIIEILKKEAKEASRPETS"
     gene            13504..13764
                     /locus_tag="PLD05_03145"
     CDS             13504..13764
                     /locus_tag="PLD05_03145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOP76456.1"
                     /translation="MYDELRDDRLIDKVGGRFKLSTLIQKRLVALMKGAPPLVDLDTK
                     DRMKIVLREILEDKIYLDLGDEVRTVADLPEPKSTVRDLRSL"
     gene            13761..14309
                     /locus_tag="PLD05_03150"
     CDS             13761..14309
                     /locus_tag="PLD05_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOP76457.1"
                     /translation="MKGHQILIGVTGGIAAYKTAALVSKLVQAGAGVTVVMTQAATQL
                     IGPKTFEALTGRPVAVDLWAPIMSHAHIELADRGEVFCIAPATANFLAKAAWGIGDDL
                     LTTTVLAFSGPIVVAPAMNSRMWQKPAVQRNLRQLREDGFIIIEPGEGYLSCGMVGPG
                     RMAEPEEIFARLGELLKGNKTA"
     gene            complement(14489..15820)
                     /locus_tag="PLD05_03155"
     CDS             complement(14489..15820)
                     /locus_tag="PLD05_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76458.1"
                     /translation="MKNAQRNDRTGKLGRREFLQTSSVLGGGILLASPLARSVHAAGS
                     DIIKIALIGAGGRGTGAASQLLNADKGVKLIAVADAFENKATSAAQRLKKQFPDQVDV
                     PPDRIFTGLLCHQGALAADPDLVVLATPPGFRPMQYRAAIEAGKHVFMEKPCCVDAPG
                     YRSLMETNKLADEKGLFVAVGLQRRHEPRYIETIKQLQSGAIGKIHFIRVYWNGSGIW
                     NRPREPGMTEMQYQVHNWYHFCWLSGDNIVEQHIHNLDVANWVLGDQHPVEANGMGGC
                     TARYLGEFKGTGQIFDHHFVEFTYADGTKVFSQCRHMKNCFNAVTEFVHGEAGIAECS
                     GRIEKGGQTVWKYDGPRPNGHQQEQIDLIAALRAGKRYNEGYIGANSSMTAVLGREAN
                     YSGKVITWDELVARGKTEFPKELSWDAPAPVTKDAEGNYPIPMPGVYNPFA"
     gene            16079..17896
                     /locus_tag="PLD05_03160"
     CDS             16079..17896
                     /locus_tag="PLD05_03160"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP76459.1"
                     /translation="MRYLSFSSRLGRSLKMMVGSLAVLLIGLVGFSLGDPHPEAGPNR
                     AEGKWASPSPVQQVSISSVTAKTQAMLTAQVRSRSQRPNIVIILADDMGYSDLGCYGG
                     EIETPHLDQLAAHGLRFTQCYNTARCWPSRAAIMTGYYAQQVRRDTVPGVPSGGRGTR
                     PAWAPLLSEILKQHGYRCYHSGKWHIDSQPWNTGFDHGYVVNDHDRFFGPKNHAEDGK
                     PLPQPKPEDNFYLTTEIARFAIKYLKEHAQQHGEQPFFLHLCFTSPHFPLQAPQDVIA
                     HYMDRYRVGWNVIQEERGKRLLQMGLVKNRPPAMEIEVGPPYHFPEALKILGPCEVNR
                     PFPWNQLTQEQQAFQVAKMAIHAAMVHIMDQAIGEVLEQLKAMNALDNTLIMFASDNG
                     ASAEIMVRGDGHDPKAPPGSAQTFLCLGPGWSSASNTPFRRHKTWVHEGGISTPLIVH
                     WPAGIKSQGELRHTPIHFIDVVPTVLDILGISLPQEWNGQPRPPFPGVSFAPLFVQDG
                     SVSHDYLWFLHEGNRAIRVGDWKLVAAHDEPWELYNLAEDRGETENLIQKEPEKAKQL
                     EQLWNRLWEQFQADARRDIPSDQEKPSPAQKTPRKSAGQ"
     gene            complement(18173..19624)
                     /locus_tag="PLD05_03165"
     CDS             complement(18173..19624)
                     /locus_tag="PLD05_03165"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOP76460.1"
                     /translation="MKTRRTLTGILVLGLMALGSQLRAADLEIELIPELDLFGDQIEV
                     IQGYQWGGEMALAFGIYDTGASVVTLSAADRAFFELQGSVVPIKVPGGAQADAIDGVV
                     IGDVSMPMTFWADGLHAVSINWSSVSWEMEFTYDLSGGATVSGVQVFVGTEEGSPQLP
                     NIAGTPIHLGTLTGSTERPAAKIDMLGYEFDFGEFFSDDPFLGPLFQGIVFYMPDLAF
                     VPSAMRLAGIAGETTDPVRIPLSLFGPTNHPDPGDETTVAPNPVQAQVSLQEALMTVA
                     DALFLFDTGASVSVITTALAEQLGLNLNSPETTLDIVGAAGAPITVPGFTLDSLSIPI
                     DSDFDGLTDGVLRFLNAPVFVLDLVEGLDGILGMNLWNTSKEMLYDPFDPLGPSLQVT
                     FLTAAREIPSDEEWELLQLLAALDPMFGQLLGIPSGPLSFPRFEMRGAGGDLGGGGEE
                     VIPEPGSLTILVLGGGLLAIAVRRSRQRPAKIA"
     gene            20077..>20558
                     /locus_tag="PLD05_03170"
     CDS             20077..>20558
                     /locus_tag="PLD05_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76461.1"
                     /translation="MSNNPQSLLPPDDQQGSSSKTSQPASETNQGRDSQETEARPNRP
                     RILIGSQRDPAAYRPRPGRPLMPFRSKPTVPGQAGGKEDTGSPGDGLAGGSPQSPLPA
                     VTTQSEVKTETQRTSQEQETESASLPEPKMETAQPAEALPPSSPPLVSSDARLFDLPE
                     "
     assembly_gap    20559..20658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20659..21762
                     /locus_tag="PLD05_03175"
     CDS             <20659..21762
                     /locus_tag="PLD05_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOP76462.1"
                     /translation="ENVFVELGLREQGIVPLRSFREPPEVGQSVEVRVVRFVPDEGIY
                     ELVLPAAAADVADWADLSEGMIVEARVTGVNQGGLECEVNRIRGFIPISQIDIFRVEK
                     PEEYVGQVFTCVVLEADAQRKNLVLSRRAILEREREQAQRLLWDSLAPGQVREGVVRK
                     LMDFGAFIDLGGVDGLLHVSQLAWGRVKHPSEILREGQTVRVRIDKVDRENRRISLGY
                     RDLISNPWEDAEKKYVPNSVWRGTVTRLMDFGAFVELEPGVEGLVHISELALKRVSRV
                     SDVVKEGDVVDVMVLSFNPQERRISLSIRAALKLKDAQTQEQAETADAGTPAEAATPE
                     PPPAPPKKRKPTGPLKGGLGSAPSGGGIFGLKW"
     gene            21766..22725
                     /gene="lipA"
                     /locus_tag="PLD05_03180"
     CDS             21766..22725
                     /gene="lipA"
                     /locus_tag="PLD05_03180"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOP76463.1"
                     /translation="MDAEHSRDAVPIIEGRVGITAAPACATTVASSGLPRWLKRRIPK
                     GNGNHFTARLIESLGLATVCEHARCPNRMECYARKTATFMILGEVCTRRCGFCAVKKG
                     KTQPVDASEPQRVAEAVKALGLRHVVITCVTRDDLPDGGAEHFAQTIAAVRAVSGATI
                     EVLPSDFAQRMESVDRVCAARPDVYNHNSETVPRLYRTVRGRKANYRWTLEMFRHIKA
                     EFPGICTKTGLMLGLGETYQELLDTFADFVAAGVDILTLGQYLRPSSDCLPVVRYVPP
                     EEFEELAQRAKQIGFKQVFAGPFVRSSYHAGEVFGAVRSTSSS"
     gene            22770..23957
                     /gene="lpxB"
                     /locus_tag="PLD05_03185"
     CDS             22770..23957
                     /gene="lpxB"
                     /locus_tag="PLD05_03185"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOP76464.1"
                     /translation="MSRTIFISAGEPSGDQHGAALLKALRRRDAGISAIGFGGPKMME
                     AGLQALADLTAHAVMWFSRPLAQLRQFWRFYRMAVESLREHRPQVVILIDFPGFHWHL
                     AKAAKKLGITVFYYGLPQLWAWAPWRVKKLRRWVDHTFAWLPFEAPWLEHHGCRVTYV
                     GHPFFDEAASHTFDSTFVARFQGSRPLLVLLPGSRDQEVSASAPLMLRTVQHLREAIP
                     SLHVAVAAFRPEQAKRVQNLAEDIQLPLEVFAGKTPELIRAADCCLAVSGSVSLELLY
                     HEKPTVIVYRIGRLAYHVQSWFRRVKYITLVNLLKYGGEQAAYLSPRDHNTPDPENAL
                     FPEFLTYRDCSRQMADCLQRWLIDTAAQEEVIKELRQLKTSLAWEGAVDRAAEAIHGF
                     LVQ"
     gene            24145..24873
                     /locus_tag="PLD05_03190"
     CDS             24145..24873
                     /locus_tag="PLD05_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOP76465.1"
                     /translation="MSSFDPHETPYDLYFTLFGIPVRVHPLFWLVAVFLGLHADDLFS
                     LLAWVLAVFIAVLVHEMGHAVAMRAFGYYPSVTLYGMGGYTRYGMRAVGVLPPRVWGR
                     ILMTAAGPAAGFLLALVLALGLSVLRVPLVVKLGPPIGVMVLANLPSPWLTVFVYYIF
                     FTTIAWGVLNLLPVLPLDGGHIMREILSIRDPHWGSFRAGQISLVVAAAVAFWSVMSG
                     SYFLAILFAYLAYSNYQALAGPRF"
     gene            complement(24951..25733)
                     /locus_tag="PLD05_03195"
     CDS             complement(24951..25733)
                     /locus_tag="PLD05_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOP76466.1"
                     /translation="MSERFFVAQLTTTGEITLSGPEAHHLIHVMRGHVGQQVVLFDGL
                     GHEAVAEITAIQKKTAVLRIAEHGTVSRELPVAVTVASPLPKGDRERFFIEKLTELGV
                     TAFIPLITHRSVVQPDENVVNRLRRIVIEASKQCGRNHLMDVTAPCKWEKLIAEPQEG
                     VTRLFAHPFSVERDGPPQPLGSDRTSGLPASLEALLTSEKVLVAVGPEGGLTPEEVGQ
                     ALAKRWIQVDLGPRILRVETAAIVLAVLVGTIRQVRAQTQGS"
     gene            25918..26793
                     /locus_tag="PLD05_03200"
     CDS             25918..26793
                     /locus_tag="PLD05_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP76467.1"
                     /translation="MTREISAVANPVVTRCLEYWGIRSQPFSPQRSAETFFLSSGAEE
                     VLVRLDYVVREGYAGAVLVGSSGVGKTLLLRRALQTWRRHRRAVVFVNATGQDRLSLT
                     TALAYELGASRSEGPAAILWQELRDHVVARLWSYEACVIICDDVARGTPDVLGLLLAL
                     GQITTNGRRPTLIGSCRHDEIHYPTVHQQSWYDIHVELPPWSVEEVQQYLCDRLREAG
                     TDPAEVFTLSAMERLAELTGGNPRDVNRLAEWCLLVAAGAEVRPIGPELVTDVFEELY
                     SQVGDLVLPRSSAVK"
     gene            complement(26836..27459)
                     /locus_tag="PLD05_03205"
     CDS             complement(26836..27459)
                     /locus_tag="PLD05_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOP76468.1"
                     /translation="MINRHKPTEPRVLGVCRRRHRPRQSPSAWILLTWLALVPTSLII
                     LTATLGCAGYRIGNATLFRPDIQTVYVPVFQSTSLRPGLGERLTEAVIKEIELRTPYK
                     VVGSLDRADSILTGRITSETKRTVVENPYDDPREIATRLQVEVQWIDQRGRVLTPEEA
                     IPVPPELSTVVAETTLVPEVGQSLATAQQAALQRLAMQIVGMMENPW"
     gene            complement(27452..28885)
                     /locus_tag="PLD05_03210"
     CDS             complement(27452..28885)
                     /locus_tag="PLD05_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76469.1"
                     /translation="MTTLDIWPDDHPRDFGTETTCAGHPTLRGTVIVVSLLIALAIGE
                     GCRSMLELPDRAKQSGYDPQEPAEEGWLFRRLLGQQEGSEHNSPSASQTKTPPAASSP
                     GNKGSSSSETQLTSGTLPEDNANSLSQLPPVDQDTVIVPPVQSEEEETEDFILFELPS
                     RFFKMFKPKQDESLARSYYEKALDLYRQRRYEEAAPLFKKAARHWQRTILEEDALFLA
                     GESYFFADRYAQADECYQEVLKKYEFTRHLDTISKRQFAIGRYWEQVELSRPSFLGIV
                     QVGDKTRPTFDTWGRAIKAYQNVRLHDPTGPLADDAIMATANAYFLKQRYEDAAYHYD
                     LLRKDYPQSEHAVNAAFLAIKAHQLTYQGPLYDGTPLERAEQAADEILAVYNSKLGDE
                     REEVIRVKNQINEEKALRYFAIGQYYDNRKYYGAARYYYRATLREFPHTKAAVMAAER
                     IRQIDGLPDKPPNRFKWLTDLFERRDD"
     gene            complement(28857..>29664)
                     /gene="recO"
                     /locus_tag="PLD05_03215"
     CDS             complement(28857..>29664)
                     /gene="recO"
                     /locus_tag="PLD05_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOP76470.1"
                     /translation="GTAPMSAEQATALVLRTYDFSESSLVVTLFTREFGKIRALAKGA
                     KRPKNPFDFALDLLALCRIVFLHKSTEALHLLTEAKLLRRFRVERSGLEGWYAALYAS
                     DLVNAMTHDEDAQPMLFDLLEDSLNRFQTRTRLKDHLLRFEWRLLSLTGHGPSLDACV
                     SCGEEIKNIAATTQNSDDTQRKTSRSEVFFALTEGGVLCPRCRAGKRAVVSVHAEALA
                     TLRLLGEDSDKEVHIPPSIYGELRGLLNQYLCHLLGQRPRMHDYLGYLAG"
     assembly_gap    29665..29764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29766..30170)
                     /locus_tag="PLD05_03220"
     CDS             complement(29766..30170)
                     /locus_tag="PLD05_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOP76471.1"
                     /translation="MPVQMQLSRIIMSEISDEQYIFLKEVDGNRTFPIVIGAFEAQSI
                     YRAVRNVSLPRPMTHDLIINIFHELQVEPQDILINELRNHTYFARLRVRHNGSLVEID
                     ARPSDAIAVAVRYNPPLPIYVAEEVLAEALGG"
     gene            complement(30394..31104)
                     /locus_tag="PLD05_03225"
     CDS             complement(30394..31104)
                     /locus_tag="PLD05_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOP76472.1"
                     /translation="MTEVQKVQPAFRSERIVPRRRARIGWYPGADYQIIQAQAVLSLL
                     FGVLSILCFLTVYVFWIPLAGLGFGWVARKEIAKAPKERLGLGMAKTGMLLSLVFGIT
                     GCTWVMYWFLAAAPPGYKLITFAELQPNPDEPKLIPQFARDLEGKKVFVWGYMIPGEK
                     QYGITDFVMVGQVSHCQFCQAQLLPTQMIEVHLTHGIKLDYTTKKIGVGGVFTCSPDA
                     LKQQFGGIVYRIEADVVR"
     gene            complement(31375..32652)
                     /locus_tag="PLD05_03230"
     CDS             complement(31375..32652)
                     /locus_tag="PLD05_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76473.1"
                     /translation="MLHPLRSLLTVLGIFIGVASVIWLLAIGEGISRKAQEQIESLGA
                     TNIIIRSIKPPSEQVQTSGLVLQYGVTRADYARLVETIPTVVRAVRIRELRQRFSYNG
                     RMVDGRLVGCTPEYQDIVRLEIDRGRFIQDSDLQDLKNYCVLAAGTAEKLFPFEDPLG
                     KVIRVEEHFYTVVGVCKPKMPSAGIGGSFAAQDFSSDVYIPITTLWWRIGDVVFTRRA
                     GSTEAEQIELSQITLQVDKTANVVPTADVVKATLAKYHPIEDYGVTVPLELLEQARTT
                     RMMFMVFMGLIAGISLVVGGIGIMNIMLATVTERTREIGIRRALGAKRRDIIRQFLVE
                     TVVLSTLGGISGILVGFLAPLLSEGAIWALQFLAPRLYNSLPEIVKTMRPVVVPASVP
                     LAFGIAVAVGIIFGLYPARRAANMDPIEALRHE"
     gene            complement(32746..33489)
                     /locus_tag="PLD05_03235"
     CDS             complement(32746..33489)
                     /locus_tag="PLD05_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76474.1"
                     /translation="MRLGASIQEITKEYHLGGEVVHALRGVSFDVPEGDYVAIMGASG
                     SGKSTLLNLLGCLDHPTSGKLFLGDENVAEASDDRLAEIRASKIGFVFQSFNLIPQLT
                     VLENIEVPLYYQRRLSPKERARCKELAEMVGLGNRLYHRPTQLSGGQQQRVAIARALV
                     NDPLFILADEPTGNLDSATTEEIMALFDDLNRQGKTIILVTHEYDVGRRARRIIRLKD
                     GRIESIIDNPQAKPRSELPEDVAKAKAPQ"
     gene            complement(33486..>34435)
                     /locus_tag="PLD05_03240"
     CDS             complement(33486..>34435)
                     /locus_tag="PLD05_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76475.1"
                     /translation="RLPDPKRMQVKTKVNESKIGLVRVGLPAKIRVDALAGVEFDGEV
                     TDVGEYPLPSSFFNSNVKDYQVIVKINNPNEQLRAGLTAQVTILAQRESNVLQVPVQA
                     VFEYAGEFYCISIDQDGKLTPVKVELGASNDRFVVIRQGLQENQEIVLNAAAYREKVG
                     LAEQKTSPQQAWTEEQAKQASETAAEPAIRTRGPAGEATEEGAGREQLGQGEGAPRPG
                     GGGPGGFNPAMIADRIFERSDANRDGKLDASEIPEDRREDLLRHDANGDGVIDRQELI
                     SGLERFRAAGGPPGGPGGFGRPRGPEGRRGPEAEGGSRP"
BASE COUNT         7002 a         9407 c        10181 g         7545 t
ORIGIN      
        1 tgaaacctgg ctcctcaccg acgggttggt gactttggaa gagatgaagg ctttgatgcc
       61 ctttgtgaca tgcggcggca gtgtttttaa ggcccaggtc gttggatatt ttgccggggg
      121 agggcctagc tgccgggtgg aggccatttt tgatgctacc gttcgtccgg cccggctatt
      181 attctggaga gatatgagcc atttgggtcg gggatttcaa gtagatgttt tggggacccc
      241 ctcaagttcc tacggcggag cggtcatccc aggggggtcg gaaagtgggg cgactttacc
      301 gggaagttga taaggtatgg cctactggtt agctattgag tggaataacg aagaattgcg
      361 ggccgtggcg gcggtgcggc ggcgggatcg tcttgttatc gagcacgcct tcgccgtccc
      421 ccttaccgac gccgttcgcc cctccgacaa cggctccccc gaatcgcggg aacagactgc
      481 ggagaatatc ggcaaacgct tggcggcggc cttggctgct cggggcattc gccgtccgac
      541 caccttcgtg gccattagcc gttccctggt cgagctccgc caaatgtcgg ttcctcccgt
      601 gtccgacgaa gagctccctg atttggtccg atttcaggcc ctccgcgagt tcacccatgt
      661 taatgaagac tggctgatcg attatctcct tcttgacgaa gacccctcgc agcctcgaaa
      721 tgccttggtg gcagccctcg ccccggagct gttccaggat atccaaaaga catgccaaat
      781 cgccaaggtc cagcctgagc ggattgttct ccgtccttgt gcgaccgcca gtttggccaa
      841 tcgctgcgtg ccgccgaagc ccgacgagat tcgcctcctg gtcgatcttt tgggagagga
      901 agtcgatctc tccgtggttg tggggaacaa attggtcttt atgcggactg cgcgggtaac
      961 gggcgatccg ctaaaggagg ccgacgcgct ggtggtcctt gtgggcgaga ttcggcggac
     1021 tatgatggcc gcccaaaatc aattgggcgg cggtcgggcc aaccatttgg tcttgtgcgg
     1081 ccaaacaccg gcgtatcaag ctttgggcca aacactacga gagcgtcttg atattgagac
     1141 gacctgcttt gatccatttt cccatttcga tttgggcccg gaagtagccg agagtttgcc
     1201 cgatcatcca ggacgcttta ccccgctgtt gggtgttctg gccgacgaac aggagaaggt
     1261 ggctcccgcg gtggatttcc ttcacccgca ccggcgtccg gaaccgccgt cacagcggaa
     1321 tcgttatgcc ctggcggggc ttgggggcct tttgatcgtc gtcttggccg gcatgtatgg
     1381 ctggttacaa cttcaggaac tcgatagccg cattgcaggg ctgcgaaatc gtcaggcggt
     1441 gctcgatcgg cagttgaaac aggcggcgga aacccgcaag ttagccgacg ccattcaagc
     1501 ctggagcggc gggcacgtca attggctgga agaactgcgg cgccttaatg agaagattcc
     1561 accagccgag gaggtgatgc tcggcaaatt gacgatggct tcgggggcac gcgggggtga
     1621 aatcagcctg gaaggtttcg ctgcccggag cgaaagcatc gaaaaaatgg aacaggccct
     1681 ggatgatcaa cagcaccggg tgatctccaa gggcaaagcc gaggacgatt cccaaaaacc
     1741 ttataaactt cgttttggca caacggtggt cattgttccg ccgtcgtcgg cttcaacatc
     1801 gcggccttca tcgcagcgcc cagccacggg ccccgccgcc tctcgtacca ctccgaacgc
     1861 tccacttgcc gggaggtgac gccatgaaac tcggacgccg agaacagatt atgatcttga
     1921 ttgtgggcac cgtggcggca atcgtcgtgg ggcaactgat ttggcgtttg gtggccacgc
     1981 ccttcacagc ccgccaaaag gccctggcaa cgctcaccag caacgtccat acgagggaac
     2041 ggcaactcaa ggaactggaa aaggccaagc aaaagctcga ggagtggcgt gcgcgctccc
     2101 ttcctgccga cccggtgctg gcccgccgct tgtatcaaaa ctggttgctg gaatgttccc
     2161 aaaaggccca actggaaaac attcaagtcg atagcgggga agtccgacgg gaacgggatt
     2221 tgttttatcg gcttcccttt acggtccgtg cccaaggatc gctggaatcg ttgacacggt
     2281 ttttgcatag tttttattcc cggaactatc tccatgccat tcagcggatc gccgtcactc
     2341 ctttaaaaga cggcaaaaag ctcgatctgc tcttcaccat cgaggcggcg gctctcgacg
     2401 atgcggtggc caaggatagt cttcccaccg gcgagttggt cgaggcaaaa ctggatgacg
     2461 tgaaagaata cacgaaaacg attgttggac ggaacgtctt tgtggcctac acaccgccac
     2521 cgccgcggga accacctcgc acaccaccgc ggacaccgcc ccggacccca ccgccggaac
     2581 ctccgccgct tgatcctatt cagttcgtct atgtgaacgg cattgtagaa gtcaacggcc
     2641 agcgggaagt gtggctctat tctcgcaccg aagacaagac gttcaagctc acggaaaacc
     2701 agactttcca ggtgggcaag acccagggac gggtcctccg catcggtatt cgtgatgtgg
     2761 aagtggaaat caatgggcag acgcgaacgc tcaccctcgg tgacaatctt cttggaaaac
     2821 ccatctccgc caccgggtga tcggctttct gggagcaatc gccgctcacg cctcaccaaa
     2881 gtgtcttagg ggataccgac gttgacgcac acttagcacg cacggaaacc cggcatggtg
     2941 tgaatcggtc aggccggttg ctcttaccgc ggcaagaaga gccggtgccg gctgcggacc
     3001 ctagccccca cgggccaaat gactatgatg aatttgatac ggttgccgtg gcttttcgga
     3061 tactctggtc ccaacgagag tgttcggtac cgccgaggtc atgccgcccc gtcttaactg
     3121 gacaggcccc ctgccgcgaa gcagcgcttt tcggatcggt tgtgccggtt ggcaatcgcc
     3181 ccgaaaaggc taacagggaa gggaaaattg gcggagggca agtatccggg gttaccacaa
     3241 catggaggcg aaagcgaaag ccaatgccca atcggctgat tcatgaaaag agcccgtacc
     3301 tgcgacagca cgcgaataat cccgtggatt ggtatccctg gggtgaggag gcctttcggc
     3361 gggcaaaaga actggatcga ccgatcttcc tctccatcgg atattccgcc tgccattggt
     3421 gccacgttat ggagaaggaa agctttgccg atccggaagt tgcggccgtc ctcaatcagc
     3481 actttgtgtc tatcaaagtc gatcgagaag aacacccggc ggtcgatgcc cagtacatgg
     3541 aggctgtgca gatcattacg ggccaagggg gctggccgtt gtcggtgttc ctcacgcccg
     3601 acttgcaacc gttctacggc gggacgtatt ggccccggga ggcacgattt ggaatgcccg
     3661 gctttttaga cgtcctgcgg gcaattgccg atgcgtggga aaagcgtcgg catgaagtcg
     3721 ttgcggaggc ggacaaggtc actgtcctat tgagcgatat ctcgaatacc cttcccggca
     3781 ggcacctcgt atctgaagag catgtccttc agaaggccga gcaggttttg gcggaggaat
     3841 tcgatccccg ctggggcggc tttggcgtcg ccccaaagtt tccccatccc tcggtaattc
     3901 gctacctcct gacccgtttc gcattccaag attcccagat ggctttgacc atggggctac
     3961 gaacactcga tgccatggcc gacgggggca tgtacgacca tgtgggcggt gggttccatc
     4021 gctattctac cgacgccatg tggatggtgc cccattttga aaagatgttg tatgacaacg
     4081 ccctcttggc ccgggcgtat ttggacgcct ggcgattgac acggcgtgag cgctacggcc
     4141 aaattgtcaa cgaaacattg gattatcttc tccgtgatat gcaccatccg gacggcggtt
     4201 tctatgcctc gcaggatgcc gatagtgagg ggcacgaggg ccagttttac ctttggacct
     4261 atgaagaaat cctgaatgtg ctggggcctg cggcggagcg cttcgccgag ttttacgaca
     4321 tcacgcggga ggggtcattt gaaggcaaga acatccccaa ccgaattcgc tcagccaatc
     4381 gttggactgc gctctcggta ggagagcgag ctcagctcga acaagagatg gccgagcttc
     4441 gccaacggct tcgagaaacg cggcagatgc gcacgccacc ggaccacgat gacaagattg
     4501 tcttggcgtg gaacgcgttc gcggcggagg tattcctcct ggcaggacgg gtgttcgacg
     4561 aggagcgtta tctgacggca gggaccatgg ccctggagtt ccttctttcg cacctgcggg
     4621 atgagcgcgg catgttttgt cattcgtggt gccagggggt ccgggggaca ccggccgtcc
     4681 tcgaggacga agccgccctg gccgtggccc tcattaccgg ttatgagaca atggcccggc
     4741 gggattggct ggaaatcgct gtgacccttg tggaagacat gcgggcacgt tttcacgatg
     4801 ccgcgacagg ggtcttcttc atgacgccgg cagaccatcc ccaccttttg tgtcgtcgtt
     4861 tagactactt tgataaccct acacctagcg ggacggccct ggccgccgag gccatgttgc
     4921 gattggggca ccttgggggg gaaccctcct atctggcgat tgccgaaggg gctttgcgtt
     4981 cccttgccgg ttggatgcaa aaatccccga tcgccgttgg tcacgccctg tttttgagac
     5041 aaacaatggt tcgcccctgg acgagttgga tgttcgtcct accctcaggg gaggttggcg
     5101 ccgtccatga gggaaaggtg ccgcccccgg tgtcgcgctg gttatcctcc ttaatgagtg
     5161 ggtataaccc atccatgatc gtgtcaatta actggttagg ggatggtagt ccaagttctg
     5221 ccacagacgg gctacccggc acaaagtctc ttcccgatcg ccctgccgtc aacggtgaac
     5281 caaccctcta catctgccaa ggtgcaagct gtggagcgcc aattgtgggg gaacaactga
     5341 tcgaaaaggt ggtacaggac catgtttcgg acgcgttgac ggcacgcaat tccgactaat
     5401 aacccccaga tttacgcctt ccaccgtact ttgccaagtc gattgggcgg cgtccccacc
     5461 ttgacgatgt caaccttccg caagaaaatt attgatagac tttgctgacc acagtacctg
     5521 cctgaggatt tttgaaattc cgcaaacttc agtaattggg cgatcgggct tgccgagaat
     5581 ttttcgaaac ttctttgttg gccggaggtt taaannnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnncaggtc atcacaagtc aattcaccga tttagcagcc acgccgggta
     5761 ttgggaagaa gaagttacag gggctactga tgctattgga acgggccgcc cacacggatc
     5821 ctacgtcgct cccggcggag acaaccgatc agacatccgt ggcagagggg cgtgaaaagt
     5881 cgccctcgga atacttgatc cggtggcaag atgtctctga actaatgtgg gccgaatggc
     5941 gcgcgaccgt ccgcaagcac ggactgcacg atattcccct cggccgactt gccccggcgc
     6001 ttaatcgtat gacccgggtg ctttggaata taccgctggg agagttcctc gacatgacca
     6061 tcgaggacct ccgggaagag cggtcatacg gcgagcgtcg gctctctgcc cttctggagg
     6121 tatttggatt gctgcatcaa atcctcaaga atgttgagcc gcaatcatac ctggcgtttg
     6181 agctagcccc gcgccgcatt gcggccatgc aacagtggat cttgcagact tggcaatccg
     6241 ggaaagtgcc gaccgaagag gaaatcaagg agagatttat cctgccgctg ttggaacaaa
     6301 cgcgactcga cgccagcgag caaaccgtga tgatggtcga gcagcgcctg ggaatcaacg
     6361 ggccaccggt gagtgtgcga cagttgggcc ggatcttcaa tttgacgcgg gcccgcatct
     6421 atcaactctt tgatgaactg gcggaaatca tgcgggttcg ttggcccctt ggtcgtgcct
     6481 acacgcaatt gctccagagc ttcatcgtat atgagtacaa tcgccggggg accggacctg
     6541 atatcagtca gctgaccatg gccatcgaga tatacttccc caagcctggc gaacggcgcc
     6601 agattgtggc cgccaaggga gggctgccag atctcagtcc gggatttgcc acgacgatgg
     6661 ccgagacatc cccgactgcc catattccag aggaaatgga tgaggactgg tgatcgccgg
     6721 gccaagcgcc ggccatcgtg aaaccttttt cccagactta gagcctatcc cgaaaaagtc
     6781 cccctctccc ttcgggagag ggttggggtg agggcaagcg aacgtcagaa tcgtttgaga
     6841 aaaccagcag cgattcgagt caccctcacc cccgacccct ctcccgccga acgggcgagg
     6901 ggaggttatg ggataggctc ttattgaaag gcccaggata gggcctcttt tttttcggaa
     6961 aactctcaaa aacggacggc ggtgccgact gagccatttt ttccattttc gtgggtccgt
     7021 ttctttatct gacatgttac aatcggcgct gttggtcgcc tgcccagtct tcttcggagt
     7081 tgactacctg caaaaggacg atcaaggtgg cggacccagg tcaacgcggt ttttctccgg
     7141 tacagcgcat cctcgaacgt tatctgcctg tttggcttgt cgttttgtgt acggtggctt
     7201 acttttggcc aagactcagc ggcgtgccgg gagacccgtt tttggcgagc aagggttacc
     7261 tgaagcatat gatcgccgtc acgatgttct tcatcggttc actcttgcgc ccagaagaac
     7321 tgttggatgt ctttcatcgc tggccgttgg tcctcttcgg aacgttggtc caatattcgt
     7381 ccatgccggc gttggcctat ctttttggac gactgatggg actcgatggg ccgtttttga
     7441 ttggcgttct catggtcggt tgtgttcccg gggcgatggc ctctaacgtc ctgacccttg
     7501 tcgcccgggg gaatgtcagt tactcagtca gcctcaccac gagtgccacg ctgctttcgc
     7561 ccttggtcgt tccctttgtg atgtggctga cggtgggaca atttgctgag tttcccgtgg
     7621 tgggcaccgg catcgaactc cttttcatgg tggtcttgcc cgtgggactg ggattcacgc
     7681 tctcccaact ttcgaggcct tggcacaagg tgggccaacg gttgggctca attgtggcca
     7741 atctggtgat cttgtggatt atcgcggttg ttgtggcggc caatcgggac acgcttcaac
     7801 aactcacggg acccttagtg ggagcgcttt tgggtatcaa cgtcggtgga tatctggccg
     7861 gcttcttggc cggttggttg gtgcggcttc cggtgccgat gcggcgggcc ttaacactgg
     7921 agatcggaat gcaaaatgcc ggattgggga cgacactggt gctggccctc tttcccgacc
     7981 aacctcaagc agccgtgccc tgtgcccttt acacctttgg gtgtatgttt acgggaacac
     8041 ttcttgcgcg aactatggcg atgtttcccg cacctggcca agggtctcag cccgaagcgg
     8101 cataacgttt atgagtgcct gctcatcgcc acggagtgtc gcggttccaa tcgaggagaa
     8161 tgcgactgga aggttcggct gtccgcgggt gggccacctg gttgaattgg acggcattcg
     8221 cgggagtgtc aaccgttgga accgtttggc cacatgagca cggggccgtc acgccgggtg
     8281 acgaaggaac cgcggaggtc tgaggggaag aggccatcgc cattggtccg agtgtgccct
     8341 ccatcgcctc gggaagcggg gcgggacgcc aaccgttgac gtccaattcc acgccgccgg
     8401 agtaaatggc ctgcgtcgca tcggaacgcc cctgagaagc tcgggcccca ccatcatatg
     8461 aggccgcaat cacaccagta cgctcggagg ctgaggcatt ccaagcggct tgggtagccg
     8521 atcgttgata aacggattcg gccgatgttg aggacgattg gggcgggcgc cattgattct
     8581 ccagcgaagc gagcaccggc tgctggggag aactgtccat cgggttgggc agggggcgaa
     8641 gttgtgggta accctgggcc acccaggtga cccaactcgt ttgcaagtcg ctgaggtcct
     8701 tgtaggggta ccattggctc aaggcagcct cccattggtt gtccttaagc ccggtttcta
     8761 ggaattggat gaaccgtcga tgcccaccct gttggatgag gaactcggcc agcgtatagc
     8821 cctgggcgta caacggcatg atgtcccggg ggtactcctc catggcgaac atccggcgga
     8881 aggagatacc gcgccgcgtt tgcagaaatt cgaggagcat tcggcgatgc ttttcgcgtt
     8941 cactgatatg ctccacggtg gtggcggcgc cttcgtcggc ccaacgggga agcggctggc
     9001 ggaaatgact ggccaagatc atgtgggtaa tctcgtgcgg cagaacggaa tccagcagtc
     9061 gttcgcggga accctgaatg gtcatttgcc agccgtaaac ctcgccgcga tcgaagacga
     9121 atgtcgtggc tccgcccgcc ccaagattcg gccccacatg gaccgtaacg gggcatggtc
     9181 gtgaccagtc tgggagcgtt ttgccaagcc aaaggcgggc caactccttc cggtagttct
     9241 cggcggcgtc gcccaattct ttcgccaact gcggatcgcc acaatggatg acaaaattgg
     9301 gggtgggata gctcgcccca ctggtcaaaa gaaggacgat aagccctgcg gcaacgggtc
     9361 gaaaacgagc gtccatgctg attgcgactt ccttgtctag gtcgaggggt catcatcctt
     9421 gggaattgag acccctcact atcgttaacg gaaatacgca gccttcacgc cgtgtaactg
     9481 aggtccgcgt caatttacca cgcccgacaa aacccgcaaa gcccgatttc tggttgaaac
     9541 ccaaaatccc gtaaaaatcg ttaccgaagg aaaaattttc caaaggttcg gtcgagggtg
     9601 gtcgataatc aatcgcgccc aggggagtgg tttgtgtcca tggaacagtt gtgcaggcaa
     9661 gggaggccgc acaggtcaac caggtaagca tggaagagag gtcgcggatg cttcggcccc
     9721 aattgcgtgt gtattatggt ccccaggaag acgaacagtt ggagcagtcc ccccattctg
     9781 gggtgacttc tgttgtgaat gttccccttc gagaagtgct ccccctttta gttgacgccg
     9841 tccggtcaaa gcgaacgtgg ttgaaagatt ttgagaacga agagattacg ctttccctcg
     9901 atctgtacga ggtcctgctg gcttaccagc attaccgccg cccatcagcg tgatacccac
     9961 cagcgagggg aaataacctc cacgaaggat ggcaacaagc ctttctaccg gcgaagccgg
    10021 taaagaggac aaaccccatc acaagattgc ggtagccatt tttatcgcct ggacatgttc
    10081 caatccttgt aggggcaacc cttgtggttg ccccagcgtt tttgcgcacg gcgcgcacca
    10141 aatgaaagcg aagtcgagga gccggcggca aggatctgac gcgcagcccg cccctaggaa
    10201 tctatgggaa atgcataaag ggcgaaaacg taccccacct cgcggtggga ctcaggctgt
    10261 ttgcctcggc cacgagagta gtgggcccca ccgaatcgcg cgggcaaggt ctgccccttg
    10321 tcaaaaacag ggatatgggc caagatgagg gttttcggtc gcgctcgggg attgcgccga
    10381 ttaagcccgt cgtccgagaa ctgtcataac acctgggagg ctgaatctct cggttctgat
    10441 ggttggccat tggcgggctg tctcttgaaa ttggtttcgt attacctcca gtccggacaa
    10501 caggttgggt gaggaaaagg agaaaagcac tatgcggcgg ccatttgttg ccggtaattg
    10561 gaagatgaat atggaacgcg cctcagccgt agcattggcc cgtgccgttg ccgacggtgc
    10621 acgtgcttat ccgcaagtgg atattgccgt ctgcccgccg tttgtctatc tggaggccgt
    10681 tgcccaggcc atcgccggaa gcccggtcgc ccttggtgcc caaaacttgt atcccgaacc
    10741 gaagggtgcg tttaccggtg agatcagcgg gccgatgctc aaggacatgg ggtgtacgtt
    10801 cgtcattctg ggccacagcg agcggcgtgc tatgggcgaa tcgagcgaat tcgtgaatga
    10861 aaaggtcttc gccgctctcg agtgcggcct aaagcccatc atttgcgtgg gggaaacgct
    10921 ccgtcaaaga aaggaaggcc gaacactaac ggtagtgcgc cgtcagattg atggcagttt
    10981 gtcggggctg tcggccgagc agatgcggca atgcgtgatc gcttacgaac cggtatgggc
    11041 cattgggacc ggtgagaatg ccactcccga tcaggccgaa gaggtgcagg cggagattcg
    11101 tcgtttgctt gccgagcgat ttgggaagga tgttgcggat tcggtgagaa ttcagtacgg
    11161 tggaagtgtg aaggcctcgg tagccgcgga tatctttgaa aagcccaatg tggatggcgg
    11221 cttgattggt ggggccagcc tgaaagcgga tgagtttctc ggtattgtgg cagcggcgca
    11281 aaaggtcaaa ggccggaacg gcggttgata cgctgaggat cggaatagga ttatgaaatt
    11341 tgtctttttg atcttgctgg tggtcacctc gctgtttttg atcctgctgg tcctaatcca
    11401 gcggggtcgc ggcggtggat tggcgggcgc atttggcggc tttggcggtc aaagtgcctt
    11461 tggtgcaaag gccggtgata cgtttacgcg gatcacaatt ggcgtagctg cgttttggat
    11521 tggcttgtgc atctttacat tgctctattt ccgctggaca caccggagtc gattggacct
    11581 tggccccacc ggaccttctg tcacgaatac cgcgccgctt gcgccaacga cgttgccgcc
    11641 ggccccaggt ggagagggtt ccggggcaag cggagggagc tcggggacga cgccttccgg
    11701 tgggacaggg caaaccggtt cgggcacaca agagagcggt tcgtaaagcg agctgctcag
    11761 gggaggaacg cctaagttcc atcgggatgt tcaggtgcgg agcggttgtc cagcggaacc
    11821 ggcgtcgcta gatgagcggg gcatggtgag tgcctttcat tgagaggaga acgctacgga
    11881 ggcagccttg ctcctgagca tgactggttt tggcgaagcc cgcagggagc acgaaggttt
    11941 ctccgtgcgg gtggaaatcc gtaccgtcaa tgcccgctat ctcaagatca gcattcgcag
    12001 taataatgcg atacccggcg gtttggaaaa tgaagtcgag gccttgattc gccgggccat
    12061 tcgccgggga accgtgaacg tcggtgtgtt tgtggaccat gagccacagg ccggcgatta
    12121 ccggattaat ggtcaagtgc ttttggggta ccttaaacag gtgtcgaact tggctgtgga
    12181 tcatgggcta gttccgccga caactttgga ctcgtttctc cttttgccgg gggttgtgag
    12241 tgatctgtct gatccggctg cccaatcgga gcgaattcgc ccggtggtaa ttgcggctgt
    12301 ggaggaggcc ctggacaggc ttcaggcgat gcggcgggca gaaggtcatg ccatgacgga
    12361 ggacctccgc gcgaactgcc agcggatttt ggagttggtg gaacgtgtgg aagcccgtgc
    12421 tccccaagtt gttgaagact atcggcagcg tctgcaagag cggatgaatc aactgctcgg
    12481 tccggcggct gcgagcctga ggccggagga tatcttgaag gaggcaagtt tctttgccga
    12541 acgctcggac atttccgaag aattggttcg cctgcggagc cacgtgcaac agttccaaga
    12601 gattttaacc aagggcggag gggaggcccc aggtcgtcgg ctggagtttc tcacccagga
    12661 gatggttcgc gaagtcaaca ccatgggggc gaaggccaat gatgtcgaga tcagccatca
    12721 tgttgtggag atgaagaccc tcgtggagcg gattcgcgag atggtccaaa atgttgagtg
    12781 acggggcaaa gcgatgagtg gacaattgcc aggacgtctg gtgattctgt cgggtccctc
    12841 cggtgtggga aagacgacag tggctaatcg cgtcctggcc gagtcccctg tgcccatggt
    12901 tcgaagtgtg tcggtaacga cgcgaccgcg gcgacccgac gaaattgatg gtgtccacta
    12961 tcgttttgta agccccgagg agttcgagcg gctgcgagct cagcaggcct tcctagagtg
    13021 ctttcaggta tttggcaacg gacattggta tgggacgctg cggcaagctg tgctctcggc
    13081 gttgtcggcg gggaaatggg tccttttggt aatcgatgtc aacggggcct tggccgtcca
    13141 ggaggaattc cccgatgctg tgagtatttt cctgctgccg aaggattggc agtccttgga
    13201 agagcgtctc cgcggtcgtg ggacggaacc cgaggaagtc gttcggcttc ggttggagcg
    13261 ggccgcggag gaattgcgat tggcggaaaa gtaccggtat cgcgtggtga atgacgattt
    13321 ggagcaaacc gttcgggata tcatcgagat tctgaagaaa gaagcgaagg aagcatcccg
    13381 gccggaaacg tcttgaggga gacaagacaa aaaagagtgc gttgaatttt caaggagaca
    13441 tcaaacgcgt tgtgcgacga acctgcccac aacgttggtc ctattatcgg aggttgagcg
    13501 aagatgtacg acgaactgcg agacgaccga ttgattgaca aagttggtgg tcgtttcaaa
    13561 ttatcgacgt tgatccagaa gcggttggtg gcgctaatga agggcgcccc accgttggtg
    13621 gatttggata ccaaagaccg gatgaagatt gtgttgcgtg agattcttga ggacaagatt
    13681 tatctggatc tcggcgatga ggtccgaacg gttgccgacc ttcccgaacc gaaatccacc
    13741 gtccgcgatt tacgctcgtt atgaaagggc atcagatcct tatcggcgtg acggggggca
    13801 ttgccgccta caagacggcc gccctggtca gcaagctggt ccaggcaggg gcgggcgtga
    13861 ctgtggtgat gacacaggcg gcgacccagc ttatcggccc caagaccttc gaggccttga
    13921 cagggcggcc tgtggcggtg gatctttggg cccccataat gtctcatgcc cacatcgagt
    13981 tggcagatcg gggtgaggtc ttttgcattg ccccggcaac ggccaatttt ttagcgaagg
    14041 cggcttgggg cattggcgac gatttgttga cgaccaccgt tctggcattt tctgggccga
    14101 ttgtggtcgc tccggccatg aatagccgca tgtggcagaa gcccgccgtt cagcggaacc
    14161 tccgccagct tcgtgaggat ggtttcatca tcatcgagcc tggcgaaggt tatctcagtt
    14221 gcgggatggt gggccccggt cggatggccg agccggagga aattttcgcc cgccttggtg
    14281 aacttctcaa ggggaacaaa actgcttgag ggaccggcct aacccatcgc ttgggcctat
    14341 cccacaacct cccctcgccc gttcggcggg agaggggtcg ggggagaggg tggctcgaac
    14401 cgctgccggt tttctcaatc gattccgacg ttcactcgcc ctcaccctaa ccctctccca
    14461 aagggagagg gtgtttcagg atagggtttt aggcgaaagg attatatacg cccggcatcg
    14521 gaatggggta attaccttcg gcatctttgg taacaggggc tggggcatcc caagagagtt
    14581 ccttgggaaa ttcggtcttt ccgcgggcga ccaattcatc ccaggtgatg actttgccgg
    14641 agtaattggc ctcgcgtccg aggaccgccg tcatgctact attggccccg atgtagccct
    14701 cgttgtagcg ttttccggcc cgcagggccg caatcaggtc gatttgctcc tgttgatgtc
    14761 cgttgggacg gggcccatcg tatttccaaa cggtttggcc acctttttcg attcggccac
    14821 tgcactcggc gatgcctgct tctccgtgga caaattccgt gacggcgttg aagcaattct
    14881 tcatgtggcg gcactggcta aagactttgg tcccgtcggc ataggtgaat tcaacgaagt
    14941 ggtggtcaaa gatttgtccg gtgcctttga attcgcccag ataacgggcg gtgcacccac
    15001 ccatgccgtt ggcctcgact gggtgctggt cgcccaggac ccaattggcc acgtccaaat
    15061 tgtggatatg ttgttccacg atgttgtcgc cgcttaacca gcagaagtga taccagttgt
    15121 gcacctggta ctgcatttcc gtcatgcccg gctcgcgcgg tcgattccaa ataccactgc
    15181 cgttccagta cacgcggatg aaatgaatct tgccgatagc ccctgactgt aattgcttga
    15241 tcgtctcgat gtagcgcggc tcatggcggc gctgtaggcc gacggccacg aaaaggcctt
    15301 tttcgtcagc caatttgttt gtttccatca acgatcggta acccggtgca tcgacgcagc
    15361 aaggcttttc cataaagaca tgcttgccgg cctcgatcgc cgctcgatac tgcatgggtc
    15421 ggaatcccgg cggagtagcc aggacgacca aatcgggatc agcagccaag gccccctggt
    15481 ggcaaagcaa accggtgaag atccggtccg gtgggacatc gacctgatca ggaaactgct
    15541 tcttcaatcg ctgtgctgca cttgtcgcct tgttttcgaa ggcatcggcc acagcgatga
    15601 gtttgacgcc cttatcagca ttcaaaagtt gcgatgccgc gccggttccc cgaccaccgg
    15661 caccgatcag ggcgattttg atgatgtcgc ttcccgccgc gtggacactg cgggcaagcg
    15721 gcgaagcgag caggattccc ccacccaaga cactcgatgt ctgcaagaat tcccgccgac
    15781 ccaacttgcc tgtgcgatcg tttctttgtg cgttcttcat agttcgggcc tttcttagag
    15841 gatcgaggtg gaatggctgc aaatctccct caacatgata gcgcaaatgc ccttcggcgc
    15901 acgacgggca gccaaggtca tcgccccaac gctggttgta tggccgcggt acattataat
    15961 ggttgccaga cggttatgac acctgcggga tagttgcatg gattgacatc cggcgcaacg
    16021 cctgctcgaa aggcccgacg tttcagaagg tacaatccta attgtgggag gcaacgcgat
    16081 gcgctatctc agtttcagct ctcggcttgg ccgctccttg aagatgatgg tgggatcgtt
    16141 ggcagtatta ctgattgggc tcgtcggctt cagcttaggc gatccgcatc cggaagcggg
    16201 gcccaatcgt gccgagggaa aatgggcgtc cccctcaccc gtgcaacaag tttcaatctc
    16261 gtctgtgacg gcaaagacgc aggccatgtt gacggcccag gtgcgctcga ggtcgcagcg
    16321 accgaatatc gtcattatcc tggccgatga catgggttat tccgatctgg gttgctacgg
    16381 cggtgaaatc gaaacgccgc acttggatca attggctgcc cacgggttgc gcttcacgca
    16441 gtgttacaac acggcccgat gctggccctc gcgggctgct atcatgacgg gctactacgc
    16501 ccagcaggtt cgacgggaca ccgtgccagg cgttcccagt ggcggacgtg ggacccggcc
    16561 ggcctgggca ccgctccttt ccgaaatcct caaacaacac ggatatcggt gttaccactc
    16621 gggaaaatgg cacatcgaca gccagccatg gaacaccggt tttgatcatg gttatgtggt
    16681 caacgaccac gatcggttct tcggtcccaa gaaccatgcc gaagatggca aacctttgcc
    16741 ccagccaaag ccggaagaca atttttactt gacaacagag attgcccgct ttgccattaa
    16801 atacttgaaa gaacatgccc agcagcacgg cgagcagccg ttctttcttc atttgtgttt
    16861 tacatcaccc catttcccgt tgcaggcgcc gcaggacgta attgcgcact acatggatcg
    16921 ataccgtgtt ggttggaatg tcatccagga ggagcgggga aagcgtcttt tgcagatggg
    16981 cttggtcaag aaccgtccgc ccgccatgga aattgaagtc ggaccgccct accatttccc
    17041 ggaggcctta aaaatcctcg ggccatgcga ggtcaatcgc cccttccctt ggaatcagct
    17101 cacccaagag cagcaggctt tccaggtggc caagatggcg attcatgccg ccatggttca
    17161 tatcatggat caggccattg gggaggtgct cgagcaactg aaggcgatga atgccctcga
    17221 caacacgctc atcatgtttg cctccgacaa cggtgccagt gccgagatta tggttcgggg
    17281 tgatggccat gatcccaagg ccccgccggg cagcgcccag acgttcctct gccttggccc
    17341 cggctggtcg agtgctagca acacgccgtt caggcgtcac aaaacctggg ttcacgaagg
    17401 ggggatttcc acgccgctga ttgtccactg gcctgccggt atcaagagtc agggcgagtt
    17461 gcgacatacg ccgatccact tcattgatgt tgttcccacg gttttggaca tcttggggat
    17521 tagcttgccg caggagtgga acggacagcc gcggccgccg tttccgggtg taagtttcgc
    17581 tcccctcttt gttcaagatg gaagtgtctc gcacgattat ctctggttcc ttcacgaggg
    17641 aaatcgggcg attcgggtgg gcgactggaa attggtggcg gcccacgacg aaccctggga
    17701 gctttacaac ctcgccgagg atcggggaga aaccgaaaac ctcatccaaa aggagccgga
    17761 aaaggccaag caacttgaac aattgtggaa ccgcctttgg gagcaatttc aagctgacgc
    17821 gcggcgggac atcccttcgg accaagagaa accctcgccg gcccagaaaa caccgcgaaa
    17881 atcggcaggt cagtagaccg gttgcgatcg atgtccgtag cgggaaagca gttcgcatgc
    17941 agttgggcac gcttgttttt gaccgcaacc gttcggttac ctgcagcctg tcccataacc
    18001 ccccctcacc ccttcggcgg gcgagaggtt agggggtgag ggtggcttca gttactgccg
    18061 gttttctcaa tggattccga cgttcacttg ccctcacccc aaccctcttc cggagcgaga
    18121 gggggactct tggggatagg ctcttggaag acgtattgtg cgtgacgcta acctacgcga
    18181 tctttgctgg tcgctgtcga gagcgtcgca cagcgattgc aagcaggccg ccgccaagca
    18241 cgaggatggt caaactgcca ggttcgggga tgacttcttc gccaccgcct cccagatcgc
    18301 cgcctgcacc ccgcatctca aaccgcggga aggacaacgg accactcgga atccccaaca
    18361 attgtccaaa catggggtcc aaagcggcaa gcagttgtag caactcccac tcctcatcgg
    18421 acgggatctc ccgcgctgcc gtgaggaatg tcacctggag cgaaggcccc aggggatcga
    18481 aggggtcgta gagcatctcc ttggaggtgt tccaaagatt catgccgagg atgccgtcca
    18541 ggccttccac gagatcgagg acaaaaacgg gtgcgttgag aaaccgcagg accccatcgg
    18601 taagcccatc aaaatcactg tcaatcggga ttgataagct atccagcgtg aaacccggca
    18661 cggtaatcgg cgccccggca gcaccaacga tgtccagtgt cgtttcaggc gaattcaagt
    18721 ttagcccgag ttgttcggcc aaagccgttg tgataacact caccgacgcc cctgtatcaa
    18781 agaggaacaa ggcatcggct actgtcatga gggcctcttg gagggagacc tgggcttgca
    18841 cgggattggg ggccaccgtc gtttcgtcgc ccggatcagg gtgatttgtt ggtccaaaca
    18901 gcgagagcgg aatccgtacc gggtcggtcg tttcacccgc tatcccagcc agacgcattg
    18961 ccgaagggac aaatgccagg tccggcatat agaaaacgat gccctggaag agtggtccca
    19021 agaaaggatc gtcagaaaag aattcgccga agtcgaactc ataacccaac atgtcaatct
    19081 tggctgcggg acgctccgtt gagccagtta gggtgcccag gtgaatgggt gtccccgcaa
    19141 tgttggggag ttgcggactt ccttcttcgg ttccgacaaa cacctgaacg cccgaaacgg
    19201 tagccccacc cgataagtca taagtgaatt ccatttccca agagactgac gaccagttaa
    19261 tggagacggc gtgcagtccg tcggcccaaa aggtcatcgg catcgaaacg tcgccaatga
    19321 caacaccgtc gatggcgtcg gcttgtgcac ctcctgggac tttaatgggc acaacggacc
    19381 cttgcaactc gaagaaagcc cgatccgcag ccgagagagt taccacgctt gcgcccgtgt
    19441 catagatgcc aaaggcgagc gccatttccc cgccccactg ataaccttgg atgacctcaa
    19501 tttgatcccc gaagaggtcc aactcaggaa taagttcaat ttcgaggtcg gccgcgcgga
    19561 gttgcgagcc cagggccatt aagcccagga caaggatacc cgtgagcgtt cgccgcgtct
    19621 tcatggcaaa gttcccccga tgcgatcgcc aaccattccc ccgaggagtt tccctggcgc
    19681 ctgtaacagc cctcccaaat tccgcccggc attataatct ccccaaattc aattgcaagc
    19741 actcgaaatg attttctaca agcgtaggag tgtcatatcc cctcttctgg gggtggcctt
    19801 atcctggcag aatctcttcg agaaggcttc aaggggtgtt tcctcttgta agaggcggct
    19861 cccccttttt gccggggcag ctttgccggt gggcgaattg gcgatgtcgc acggcgcttg
    19921 ggcctgccgc atcgctgcct ggagcaaata cccgcagggc ggtagaataa agtgtttggc
    19981 attggccccg gaccgtggcc tttggcagta aaaggccgcg ggccatggtg gctttgcgat
    20041 ttggtgacca ccagttgtga tgtgagtttt ggtccgatga gtaacaatcc gcagtccttg
    20101 cttcctcctg atgaccagca gggttcctcc agtaagactt ctcagccggc gagtgagacg
    20161 aaccaaggaa gggattccca ggaaacagaa gcccgcccca atcgtccgcg aatcctgatt
    20221 ggttcacagc gggacccggc agcctatcgg cctcgcccag ggcggccgct gatgccgttt
    20281 cgaagcaaac ccacggttcc cgggcaggca ggaggtaagg aggatacggg ctctcccggc
    20341 gatggtctcg ccggtggttc cccacagtct cccctcccgg cggtgacgac ccaatcagag
    20401 gtcaagacgg agacccagag aacatcgcag gagcaggaga ccgagtcggc gtctttgccg
    20461 gagccgaaaa tggagacggc gcaacccgcg gaggcattgc cgccgagtag tccgccgctt
    20521 gtctcttccg atgccaggtt attcgatctt cccgaggann nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnga gaacgtcttt gtggagttgg ggctgcggga gcagggaatc
    20701 gttccactgc ggtcatttcg tgaaccgccg gaggtcggtc aatcggtgga ggtccgcgtg
    20761 gtacgatttg tccccgatga ggggatttac gagcttgtcc tgcccgcggc agcggccgat
    20821 gttgccgatt gggccgatct gtcggaagga atgatcgtcg aggcccgcgt gaccggtgtc
    20881 aatcaagggg gcttggagtg cgaagtcaac cgtatccggg gattcattcc tatcagccaa
    20941 attgatatct tccgggtgga aaagcccgaa gaatacgtgg gccaagtctt cacctgtgtc
    21001 gttctggagg ccgatgccca gaggaaaaat ctggtgctga gtcgccgggc tattttggag
    21061 cgtgaacggg aacaggctca acggttgcta tgggattctc tcgcaccagg ccaggtccgc
    21121 gagggcgttg tgcggaagct gatggacttt ggggccttca ttgacttagg cggcgttgat
    21181 ggtttgctcc acgtcagtca attggcgtgg ggacgtgtca agcatccaag cgagattctc
    21241 cgcgaagggc aaaccgtgag ggtccgtatc gacaaggtcg atcgtgaaaa tcgccgcatc
    21301 agtttgggtt accgtgattt gatttcgaac ccctgggaag acgccgagaa aaagtatgtg
    21361 ccgaattctg tttggcgggg aacggttacg cggctgatgg attttggggc cttcgtcgag
    21421 cttgagccgg gcgtggaggg cctggtgcat atctcggaac tggcactcaa gcgggtgagc
    21481 cgtgtctcgg acgtggtcaa agagggcgat gtcgtggatg tgatggtgct ttcctttaat
    21541 ccgcaggaac gacggattag cctttccata cgagcggcct tgaaactgaa agacgcccag
    21601 acgcaagagc aggccgagac tgctgacgcg gggacaccgg cggaggcggc aacccccgaa
    21661 cccccgcccg ctccccccaa gaagcgcaaa cctaccgggc cgctcaaggg aggcttggga
    21721 agtgcaccct ccggaggagg tatctttggt ttgaagtggt gatggatgga tgccgaacat
    21781 tcgcgtgacg ctgttcccat aatcgaagga cgagtcggga tcacggccgc accggcctgt
    21841 gccacgacgg ttgcatcgag cggtcttccg cgatggctca aacgccgtat tccaaaagga
    21901 aacggcaatc atttcaccgc ccggctgatt gagtccctcg gcctggctac cgtgtgcgag
    21961 catgcccgat gccccaatcg catggaatgt tatgcgcgga agacggccac cttcatgatc
    22021 ctgggggagg tctgtacacg ccgctgcggc ttttgtgccg tcaaaaaagg aaagacacag
    22081 cccgtggatg cttccgagcc ccagcgcgtc gcggaggccg tcaaggccct ggggttgcgt
    22141 cacgtggtga tcacatgcgt gacgcgggac gatttacctg atggtggggc ggagcatttt
    22201 gcccagacga tcgccgctgt gcgggcggtc agcggtgcca ccatcgaggt cttgccttcc
    22261 gatttcgcac aacgcatgga gtccgtggac cgtgtttgtg cggcccgacc agacgtttac
    22321 aatcacaact cggaaaccgt accgcggctc tatcgtaccg tcagggggcg caaggctaac
    22381 tatcgttgga cgctggagat gttccgccat atcaaggccg aatttccggg catctgcaca
    22441 aagacgggtt tgatgctggg attgggcgaa acctaccagg aattgcttga tacttttgcc
    22501 gattttgtgg cggctggtgt ggacattttg acattgggcc aatacttgcg gccctcgtcc
    22561 gattgcctgc ctgttgtccg ctatgtgccc ccggaggagt tcgaggaact ggcccagcgg
    22621 gcgaaacaga tcgggttcaa gcaggtcttt gccggaccat ttgtgcgatc gagttatcat
    22681 gccggtgagg tgtttggagc ggtgcggagt acctcttcat cctgatcggg agctatggga
    22741 acacggtgtc tcatcgcagg gaggcgtcta tgtcacgaac gatcttcatt tcggccggcg
    22801 agcccagcgg tgatcaacac ggtgccgcgc ttttgaaggc cttgcgacgg cgggacgccg
    22861 gcatatcggc catcggattt ggcggtccca agatgatgga ggcggggtta caagccttgg
    22921 ccgatcttac ggcccacgcc gtgatgtggt tttcccgacc gcttgctcaa cttcggcagt
    22981 tttggcgatt ctatcgcatg gcggtggaat cgcttcgcga acatcgcccc caggttgtca
    23041 ttttgatcga ctttccgggg tttcactggc acctggcgaa ggcggccaag aaactcggaa
    23101 tcacggtgtt ctattatggt cttccgcagc tttgggcctg ggcgccgtgg cgggtgaaaa
    23161 agctccgccg ctgggtggat cacacgtttg cctggcttcc ttttgaggcc ccctggttgg
    23221 aacatcacgg ttgccgtgtg acctacgtgg gacatccctt tttcgacgag gcggcatccc
    23281 acacattcga ctcgacgttt gtcgcccgct ttcaggggtc gcgtcctttg ctcgttctct
    23341 tgccgggttc gcgggatcag gaggtctcgg cctctgcccc gctcatgttg cggactgtgc
    23401 aacatttgcg tgaggccatc cccagtttac acgtcgcagt ggcggccttc cgaccggagc
    23461 aggcgaaacg agtccaaaac cttgccgagg acatacaact gcccttggag gtctttgctg
    23521 ggaagacccc tgaattgatt cgggcggcgg actgctgttt agccgtttct ggctccgtct
    23581 cgcttgaact tctttaccac gaaaagccta cggtgattgt ataccgcatc ggacgattgg
    23641 cttaccacgt tcaatcgtgg tttcgtcgtg tgaagtacat tacgctagtt aacttgctca
    23701 agtatggagg cgagcaggct gcctatcttt ctccccggga tcacaatacg cccgatcctg
    23761 agaacgccct attcccggag ttcttgacct accgcgattg cagccggcag atggccgatt
    23821 gcctccagcg atggttaatc gacacggcag cgcaggagga ggtgataaaa gaactgcggc
    23881 agttgaaaac atctttggcc tgggaagggg cagtggatcg agcggccgaa gcgatccacg
    23941 gttttctggt gcaatgatgc agtcccgggg tgtctggggc accgtaccct tttgacaaag
    24001 tagaattgtc aaacccacag tatttaacga aaatgggaac cattgtactt tctgtgagcg
    24061 gatggggcaa aacggtcatg tgggaaacgg gacgattagt ggtgctcata ggaactggta
    24121 aatgacaagg attgggatga ggttgtgagt tcattcgatc cccacgaaac gccctatgat
    24181 ctttatttca cgctctttgg tattcccgtg cgggttcatc cgctgttttg gcttgtggcc
    24241 gtgttcctgg ggctacacgc ggacgacctt ttctcgcttt tggcttgggt tctagcggtt
    24301 tttatcgcgg tgctggttca cgagatgggc catgccgtgg cgatgcgggc attcggctat
    24361 tatccatcgg tcacgcttta tggaatggga ggatacacgc gatatgggat gcgggccgtg
    24421 ggtgtgttgc cgcctcgggt ctggggacgg atcctgatga cggccgccgg acccgccgcg
    24481 ggatttctct tggcccttgt tttggccctc ggtttaagcg tcctccgggt gccgctcgtc
    24541 gtaaaacttg gtccgcctat cggcgtcatg gtccttgcca atttgccgtc cccttggtta
    24601 accgtgtttg tctattacat tttcttcacc accattgcct ggggtgtctt gaatctgttg
    24661 cctgttcttc ccctcgatgg tggccatatt atgcgggaga tactgagcat ccgggatccg
    24721 cattggggga gctttcgggc agggcagatt tcgttggtgg tggctgccgc cgtggcattc
    24781 tggtccgtga tgtccggcag ttatttctta gcgattcttt ttgcctatct tgcttattcc
    24841 aactatcaag ccctcgctgg cccgcgattc tgatccaatt ttgatgggcg taaacagcgc
    24901 ccgttagccg gttgcgcgac caattacggc aaactacttt ataggcctct tcatgacccc
    24961 tgtgtttggg ctcgcacctg ccggatagtg ccaacaagga ccgccaaaac aatggcggcg
    25021 gtttccaccc gcaaaatcct cgggccaaga tcgacctgaa tccacctctt ggcgagggct
    25081 tggccaactt cctcgggggt taatcctcct tccggtccca ccgcaacaag gaccttttcc
    25141 gaggtgagaa gagcctcaag acttgccgga agaccacttg ttcggtcgct gcccagtggc
    25201 tggggcggcc cgtctcgctc aacggaaaaa gggtgcgcga aaagcctcgt gactccctct
    25261 tgcggctcgg cgataagctt ttcccatttg catggggctg ttacatccat aaggtgattc
    25321 cgcccacatt gtttgctggc ctcgatcaca atccgccgga ggcgattgac gacgttctcg
    25381 tcgggctgga ccacactccg gtgggtgatc aggggtatga aggcggtgac gcccagttcg
    25441 gtcaattttt caatgaagaa tcgttcacga tcacctttgg ggagggggct ggccaccgta
    25501 acggcgacgg gcaattcccg cgataccgtc ccatgttccg caatccgcaa cacggcggtc
    25561 ttcttttgga tagccgtgat ctccgctacg gcctcgtgtc ccaaaccatc aaaaaggact
    25621 acctgctggc cgacgtggcc cctcatgacg tggatcaagt gatgggcctc gggaccgcta
    25681 agcgtaatct cgccggtggt ggtaagttgg gcaacaaaaa agcgttctga catcgtttga
    25741 cataggaggc ttggttgcgg atgaactgcg gcaataccgg agtatgccgg gccgctgtca
    25801 aactttatag attttagcag aaagtctgac gatgaagaga ataaggggta ttacggccac
    25861 gtactgcgaa gggccgaagc tcaaagtatc ctgacatcca cccgttatgg tgacaatatg
    25921 acgcgtgaaa tctcggccgt ggcaaatccg gtggtgacgc gatgcctgga gtattggggc
    25981 attcgcagtc aacctttcag cccgcagcgt tcggcggaga cgttcttttt atcgagcggg
    26041 gctgaggagg tcctggtccg tctcgactac gtcgtaaggg aggggtatgc cggggccgtc
    26101 ttggtgggtt catctggggt gggcaagaca ctccttctcc gccgtgctct ccaaacttgg
    26161 cggcggcacc gccgggccgt ggttttcgtt aatgccaccg gtcaagatcg gctttcgctt
    26221 accacggcat tggcctatga gttgggggcc agccgcagcg aaggaccggc cgccatcctt
    26281 tggcaggaac tgcgggatca cgtggtggct cggctttggt cgtatgaggc ctgcgtgatc
    26341 atctgtgacg atgtggccag aggcactccc gatgtcctcg gcctcctttt ggccttggga
    26401 cagattacaa cgaatggccg tcggccaacc ttgatcggct cttgccggca tgatgagatt
    26461 cattatccca ccgtgcacca acaatcttgg tacgacatcc acgtggagct tccgccctgg
    26521 tcggttgagg aggttcagca gtacttatgc gaccgactcc gtgaggctgg caccgatccg
    26581 gctgaggtct tcaccctatc ggccatggaa cgcctcgccg aacttacggg aggcaatcct
    26641 cgtgacgtga accgactggc cgagtggtgc ctccttgtgg cggcaggtgc ggaggtgcgg
    26701 ccgattgggc cggaactggt gacggatgtc tttgaggagc tatattcgca agtgggcgac
    26761 ttagtcttac cacggtcaag cgcggttaaa tagtttactc tgaggatcgg cgtactcacc
    26821 gcgcggatgc ccggtttacc acgggttttc catcatgccg acgatctgca tggcgagccg
    26881 ttggagagcg gcctgttggg ccgtggccaa ggattgaccg acttcgggga cgagggttgt
    26941 ctcggccaca acggtgctca gttccggcgg aacagggatg gcttcttccg gcgtgaggac
    27001 ccttccccgc tggtcaatcc attgaacctc gacctgcaag cgtgtggcga tctcgcgggg
    27061 atcgtcgtag ggattttcga caaccgtgcg tttggtctcg gatgtgatcc gccctgtcag
    27121 gatgctatcc gcccgatcca gcgagcctac gaccttgtaa ggggtacgca attcgatctc
    27181 tttgatcacg gcttctgtca gtcgttctcc taaccccggg cgaaggctcg tcgattgaaa
    27241 gaccgggaca tagaccgtct gaatatccgg ccggaagagt gtcgcatttc cgattcggta
    27301 cccggcgcag cccagtgttg ctgtgaggat gatcagactt gtgggcacca aggcaagcca
    27361 tgtcaaaaga atccaagcgg agggcgattg ccgcggtcga tgcctccgcc ggcagacgcc
    27421 gagcacgcgc ggttccgttg gcttgtgtcg gttaatcatc acggcgttca aaaaggtcgg
    27481 tcaaccactt gaagcggttg ggaggtttat cgggaagacc gtcaatctgg cgaatgcgtt
    27541 ctgcggccat gacggcggcc ttggtgtggg ggaactcccg cagtgttgcc cggtagtagt
    27601 agcgggcggc cccatagtat ttgcgattgt cataatactg gccaatagcg aagtagcgga
    27661 gggccttttc ctcattgatc tgattcttga cgcggatgac ttcctcccgc tcgtctccta
    27721 acttgctgtt atagacggcc aggatttcgt ccgcggcctg ctcggcccgt tccaagggag
    27781 ttccatcata gagtggccct tgataagtca actgatgcgc tttgatggcc aaaaaggccg
    27841 cgttgacagc atgttcactt tggggatagt cttttcgcaa gagatcataa tgataggctg
    27901 cgtcctcgta tcgctgtttt agaaaatagg cgttggcggt ggccatgatg gcgtcgtctg
    27961 ccaaaggccc ggtgggatcg tggagccgca cattctgata cgccttgatg gcccgtcccc
    28021 aggtgtcgaa ggttggccgt gttttgtcac cgacttgaac gatacccaaa aatgacggtc
    28081 gagaaagctc cacctgttcc caatatcgcc cgatagcgaa ttgacgtttg gaaatggtgt
    28141 ctaggtgacg ggtgaactcg tatttcttga ggacctcttg ataacactcg tcggcttggg
    28201 cgtagcgatc ggcaaagaaa taagattctc ccgccagaaa gagggcatct tcttcgagga
    28261 tggtccgttg ccaatggcga gccgcttttt tgaagagggg agccgcctct tcgtaccgcc
    28321 gctgccggta tagatcgagc gccttctcgt agtaagaccg agccagggac tcgtcctgct
    28381 tgggcttgaa catcttgaag aaccggctgg gaagttcaaa gaggatgaag tcctccgtct
    28441 cttcttcctc gctttgaacc ggcggcacaa tcacggtgtc ctgatcgacc gggggaagct
    28501 gcgagagaga attagcatta tcttccggaa gggtcccaga ggttaactga gtttctgatg
    28561 aagaagaccc tttgttcccg ggggaactgg ctgccggagg agtctttgtc tgggacgcgg
    28621 agggagagtt gtgctctgat ccctcctgct gtcctaggag acggcgaaaa agccaacctt
    28681 cttcggccgg ctcctgcgga tcgtatccgc tttgtttggc gcggtcgggg agctccaaca
    28741 tgctacggca tccctcgccg atcgccaggg caatcaatag gctcacgacg atgacggtac
    28801 cccgaagggt tgggtgtccc gcgcacgtcg tttccgtgcc gaagtctcgc gggtgatcat
    28861 ccggccaaat atccaaggta gtcatgcatc cgtggccttt ggcccaaaag atgacagaga
    28921 tactggttta gcaatcctcg taattctcca taaatcgatg gcggaatatg cacttctttg
    28981 tcggagtctt cccccaatag ccgcagtgtg gcaagcgcct cggcgtgaac cgagacgact
    29041 gcccgcttgc cggctcggca tcggggacag agcacgcctc cctctgtcaa ggcgaagaac
    29101 acctccgacc gggaagtctt tctttgcgta tcatcgctgt tttgagtcgt agccgcgatg
    29161 ttcttgatct cttcaccaca actcacgcac gcatccaacg aggggccgtg tcccgtcagc
    29221 gacagcaatc gccactcaaa tcgcagcaga tgatctttca gtcttgttcg cgtttgaaag
    29281 cggtttaggg aatcctccaa aaggtcaaac agcatgggct gagcgtcctc atcgtgggtc
    29341 atggcattga ccagatcaga cgcatataac gccgcatacc agccttccag gccactccgt
    29401 tctacccgaa agcgacgcaa tagcttcgcc tcggtgagga gatggagggc ctcggtcgat
    29461 ttgtggagga acactatccg acaaagggcg aggaggtcaa gggcaaagtc gaaggggttc
    29521 ttgggtcttt tagctccttt ggccaaagcc cgtattttgc caaattcccg cgtaaacagg
    29581 gtgaccacca aactgctttc gctgaagtcg tacgttcgca ggaccaaggc ggttgcttgt
    29641 tcggcactca tgggggcggt cccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29761 nnnncttagc cgccgagggc ctcggccagg acctcttctg cgacgtagat tggaagcgga
    29821 ggattgtacc gaactgccac ggcaatggca tcagaaggtc gggcgtcaat ctccaccaag
    29881 gagccgttgt ggcggacgcg tagcctggcg aaatacgtat gattgcggag ctcgttgatc
    29941 aagatgtctt gcggctcgac ctgcaattcg tggaagatat tgataatcaa atcgtgcgtc
    30001 atcggcctgg ggaggctcac attacggacg gcccgataga tgctctgcgc ttcaaatgcc
    30061 ccaatgacga taggaaaagt ccgattccca tccacctctt tgaggaagat gtattgctcg
    30121 tcgctgattt cgctcatgat aattcgggaa agctgcatct ggacgggcat cgagtgcctc
    30181 cgctgtcctg caaacgaccg cacatgcctt cggggacgtc tgttgcgctg aactttgctt
    30241 tcaaccccag ttttccacct tactttttta ttctatcgaa ggaacggtat cgctcaccag
    30301 acgcgggttg gaggatcggc gaaagtttgt ctcggcttgt ggtgctgcca attcttgccg
    30361 cacgatgggc acctacgcag tataggcaaa cctttaccga accacgtctg cctcaatgcg
    30421 atagacgatc cctccgaatt gctgcttgag agcgtctgga ctacaggtaa aaacaccgcc
    30481 cacgccgatc ttcttcgtcg tgtaatccag tttgatgccg tgcgtcaggt ggacttcgat
    30541 catctgcgtg ggaagtaatt gggcctgaca aaactgacag tgtgatactt gtcccaccat
    30601 cacgaaatct gtaatgccgt attgcttttc cccggggatc atgtatcccc agacaaagac
    30661 cttcttacct tctaggtccc gggcgaattg gggaatcagc ttgggctcgt cagggttcgg
    30721 ctgcagctcg gcaaaggtga tcaatttgta tcccggcggg gccgccgcca aaaaccaata
    30781 catgacccag gtgcagcctg tgatcccaaa caccaacgac aaaagcatcc ccgtcttggc
    30841 catccccagt ccgagacgtt ctttgggtgc tttggcgatc tcttttcgtg ccacccaacc
    30901 gaaacccagt ccggccaggg gaatccaaaa aacatagaca gtcagaaaac agaggataga
    30961 aaggacgccg aagaggaggc tcaacacggc ctgggcttga atgatctgat agtccgcccc
    31021 agggtaccac ccgatccgcg cacgacggcg tggaacgatt cgctcacttc gaaacgcagg
    31081 ctggactttt tgcacttcgg tcatgacgat gaatcccgaa aaacctcgca ttccgcggaa
    31141 atacgctcgt gtattccgac ggagcttaaa cctccgcggg tagcatggcc attaagaacc
    31201 tcgccttatg gcactggaca gcgaaaacgt gcttcccggg gggaataaac ccaccataaa
    31261 acctgatcgc aacgggccac cgtagtcttt cacctttccc cattattgcc gattcgcgga
    31321 aagagttcga ggaaattgca atggggcaag cggaaagacc cacggacggc gccatcactc
    31381 gtggcgcagg gcctcaatgg gatccatatt tgccgcacgt cgggcaggat acagcccgaa
    31441 gatgatcccc accgccactg caatcccaaa cgccaggggc acggacgccg gaacaaccac
    31501 cggccgcatg gttttgacaa tttcgggcag cgagttataa agtcgcgggg caaggaactg
    31561 caatgcccag atcgctccct cactcaaaag cggagccaag aaaccgacga ggatgcccga
    31621 aatgcccccc aacgtggaaa ggaccacggt ttccaccagg aattggcgga tgatgtctcg
    31681 ccgcttggca cccagggcac gccggatgcc gatttcacgc gttcgttccg tgacggtagc
    31741 cagcatgatg ttcataatgc cgatgccgcc cacgaccaac gaaatcccgg caatcaaccc
    31801 catgaagacc atgaacatca tccgcgttgt gcgtgcctgc tcaagcagtt ccaaagggac
    31861 ggtcacaccg taatcttcga tgggatggta tttggccagc gtcgccttga cgacatcggc
    31921 ggtggggacg acgttggcgg tcttatcgac ctggagggtg atttgactaa gttcgatctg
    31981 ctcggcctcc gtggagccgg cccggcgggt gaagacgacg tccccgatgc gccaccaaag
    32041 ggtcgtgatg ggaatataca catcgctgga gaagtcctgg gcggcaaaag agccaccgat
    32101 cccagccgag ggcatctttg gcttgcacac gccgaccacg gtatagaagt gttcttccac
    32161 ccggatgacc ttacccaatg ggtcctcaaa aggaaagagc ttctccgcgg tccccgccgc
    32221 aaggacacag tagttcttga ggtcctgaag gtccgaatct tggataaaac gtccgcggtc
    32281 gatttccaaa cgaacgatgt cctggtattc aggcgtgcaa ccaaccaggc gtccatcgac
    32341 cattcggcca ttgtaactga atcgctggcg cagctcccga atacggaccg cccgcacaac
    32401 cgtgggaatg gtctcaacga gtcgggcata atctgcgcgg gtgacgccat actgaaggac
    32461 aaggcccgac gtctgcacct gctcggacgg cggcttgatc gagcgaatga taatattggt
    32521 ggccccaagg ctttcaattt gttcctgggc cttgcgacta atcccctcac cgatggcgag
    32581 caaccagatg acgctcgcca caccgatgaa gatacccaat acggtcaaaa gtgaccgtaa
    32641 cgggtggagc atcaagctct tgagtcctag ttggatcgtc ctataccaaa ggatcaacat
    32701 ggtttcttac tgggatgtgt ggctgcattc cttggggaaa gcaagttatt gtggtgcttt
    32761 ggccttggca acgtcttctg gcagctcgct ccgtggcttg gcctgggggt tatcaataat
    32821 cgattcgatg cgtccgtctt tgagacgaat aatcctccgc gcccttcgcc ccacgtcgta
    32881 ctcgtgggta accaagatga tcgttttgcc ctgccgattg aggtcgtcga agagcgccat
    32941 gatctcttcg gtggtggccg agtccaaatt ccccgtcggt tcatccgcca gaatgaagag
    33001 cggatcgtta accagtgccc gcgcaatggc cacacgttgc tgctgtcccc ccgaaagctg
    33061 agtcggccgg tggtaaaggc gattgcccag gccaaccatc tcggccagtt ccttacagcg
    33121 tgctcgctcc ttgggtgaaa gccgccgctg gtagtacagg gggacctcga tattctccaa
    33181 cacggtcagt tggggaatca gattgaatga ctgaaagaca aaaccgatct tcgatgctcg
    33241 aatctcggcc aggcgatcgt cggaggcctc agcaacattt tcatcgccaa gaaagagttt
    33301 gcccgaggta gggtgatcca agcagcccaa tagattcaaa agcgtgctct tacccgatcc
    33361 agaggcaccc ataatggcga cgtaatcgcc ttcgggcaca tcaaaggaga cgccgcgcag
    33421 ggcgtgaacg acctcaccac ctaagtgata ctcttttgtg atctcctgga tggatgcccc
    33481 taacctcatg gtcggctgcc tccttcggcc tcaggaccac ggcgtccctc gggacctcgg
    33541 gggcgtccaa aaccgccggg accacccggg ggaccgccgg cagcgcgaaa gcgttccagc
    33601 cccgagatga gttcctgtcg gtcgatcacc ccgtcaccgt ttgcgtcatg ccgaaggagg
    33661 tcttcacgac ggtcctcggg gatttcggaa gcgtcgagtt tcccatctcg gttggcgtcc
    33721 gagcgttcaa agatgcgatc ggcgatcatg gccgggttga acccgcccgg acctccgccc
    33781 cccggccgcg gcgctccttc accttgacct agctgctccc ggccggcccc ctcctccgtg
    33841 gcttcacctg ccggtccccg tgtacgaatc gccggttcgg ctgccgtctc gctggcctgt
    33901 tttgcctgct cttcggtcca ggcctgctgc ggcgacgtct tttgctcggc caatcccacc
    33961 ttttcgcggt acgctgcggc gttcaagacg atctcttgat tttcttgcaa gccttgccgg
    34021 atcaccacaa atcggtcgtt gcttgccccg agttccacct ttaccggtgt gagctttcca
    34081 tcctgatcga tggaaataca atagaactct cccgcgtact cgaatacggc ctgcaccgga
    34141 acctggagca cgttgctttc tcgttgggca aggatggtca cttgggcggt tagtcccgcc
    34201 cggagttgct cattggggtt gttaatcttc acaatcacct ggtagtcctt cacgttcgaa
    34261 ttaaagaacg agctgggcaa ggggtattcg cccacgtccg tgacctcgcc atcaaactct
    34321 accccagcca aggcatccac gcgaatcttt gccggcaagc cgactcgaac cagaccaatt
    34381 ttcgattcat tgaccttggt tttgacctgc atcctcttgg gatcgggaag gcgga
//
LOCUS       DAOSUT010000021        33897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_896_length_33897_cov_9.654896, whole genome shotgun sequence.
ACCESSION   DAOSUT010000021 DAOSUT010000000
VERSION     DAOSUT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 33897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33897
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_33897_cov_9.654896"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1430
                     /locus_tag="PLD05_03245"
     CDS             129..1430
                     /locus_tag="PLD05_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76476.1"
                     /translation="MNLLPIVICYLLLALSSTIALAVILRRRSPPRRRVSDFVDIAAL
                     GSYAVLGVLGALSTAVGHPPGLPSAVKDALNWLLTPATVMTLWLLAMVILLWRPSIFS
                     SPWIFWHAATWGSLWFLLSMTDSHFAALVLKPDHIAVVFLVALTGFFTWLGLRQSVLN
                     DQRLDRGRKPREATKPEQTRVFVWPDLVYIELIAILGMTCVLGVWSLAVPAPLEAPAD
                     PAWTPNPAKAPWYFVGLQELLVYFEPWFAGVIVPVIITVGLCLIPYLRRRDQRSGYYS
                     LKGRRFSVAIFLFGLGLWVLLILIGTFFRGPDWRLYGLYESRMVRRFDELRAVPLSFL
                     VWRLVLRTADTSLEMTDIPSSPFLRELPGLVIIAFLFFVFPFFAKHTFGRKWADRLGK
                     TGYWTVAILLAMMTLIPIKMYANWLFDINYFIYLPEIGLSL"
     gene            1499..5419
                     /locus_tag="PLD05_03250"
     CDS             1499..5419
                     /locus_tag="PLD05_03250"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOP76477.1"
                     /translation="MDRKVDHHYPIWLLHAICLLACAAIIVIGVILATREQRQAWRGF
                     QRHGLRILESAMAHDAKSGRMTPQGECISFGTQKLPLAVMDDEPLAMTIGRMHELILP
                     DLPHRLGPVESPRRERCMTCHVLIDWTGDTLSVSDSSSQDRGPIWTRPTRVSEPISEV
                     FPLETILEIVLPVVITGKAGDGVPSNREEKADTSLTFGFSWVEPGLLKGDEPRIGTVL
                     RGSPADSAGLRPGDLICAIGNSPVETKDQAETLLSESVRQLKGLSQDLKGDFVKTYYP
                     APFGSGESVPGIAIKVRRGLPQPWAGHPHPELYVAERSPHPVRRFGCTICHDGQGLAL
                     DYRQAEHPSPIVEGSSLGKRCYGYAINVRESIEPMILTELAESRCLRCHDRAFDLAET
                     DIRWEPIAKHLVEGRRLVEKFGCFSCHEMQRDSAPGEPQLGPVSPATEIAEELLALSS
                     TPQELKEIAVRFLETPNDKELLERLRKETTRWQDLLESDTRRFPEDREIFVTRQQVRQ
                     LVGALQAANVISGGWSKVGPSLRYVASKLAPKTIERVISEPQSVRPESRMPQIFGLTD
                     HLSEGPAQHRKQFEQVEIAGIVAYLAGQSHPLPSNRTETRAPPQDKLGSRSLADAGRI
                     LFATQGCLACHRHRDFPEGTSDVGPDLSDLGQRLVCDTGREWLRLWLTSPQTLSPSTR
                     MPQPQFRDDAWIYRVFEPAESKPRRVTSSEVIDALVAFLLSGESSTNGSQQPEKLPVN
                     AEWLDEVVLEYLSSDLPREKAQGIVRNGATVADLGKYSLQVALELTELLGEPTSDKKL
                     RYVGRKAIARYGCYGCHNISGFEGWPPIGPSLSQFGSKPVALLDFGAVAEGLLSTPEG
                     RHIVQRRKTPLEDRLSSLDFLGRETWLWLKLISPRAFDFKAMEGKPILQHSRMGRFPL
                     SGEERQAVMTFILGLEGRPVPAKYDPYSASRKALALGREIIERRACDRCHALRPEQWF
                     FRFSEEDVVREMGGDYDPIGRQTEAEKASAMPPRRPLGYALAMGQAERDASGQIIEDR
                     DDFDNPLYFFMPWRPVAFGAHIWPVGEASIPIASTQLLGKRDQDGGAFAFMLYPTVVS
                     LIQQIQLSMGLKETMGCLPPPLYYEGVMVQPSWLSQYLKEPYPIRPAIPLAMPRYWLT
                     NEEVQILVDYFSALAEKTGESDTSWKTTETQLILREQQWPGRLDAAWHLIHDTKTYCA
                     KCHISSDNMEDRLALPTEAPRLDGVYRRLQHDFIRAWVVQPKAIRPYTAMPVNFPENG
                     IPLDRSVFDADSRVQLEAVVDLLQNYDWYLKQRVRQELGPAQSQ"
     gene            5646..6461
                     /locus_tag="PLD05_03255"
     CDS             5646..6461
                     /locus_tag="PLD05_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76478.1"
                     /translation="MINKFRSTRRAFLKSSLSLAVGTAAVGLTRKATFGITESARGEV
                     RGRFVYDGQPPERKKLKVDKDVDCCGKYDIRDESLMVGLENGLANVFVYVRTRGIPIP
                     PDMAEQIPKRVVLDNHECIFKPHCMAIWYPVQEFYIVNSDPIAQNVAFSPLGDLPANI
                     ILAPAPGDNIDAVWRFRRSQTEPVLIVCNYHPWESAYILPRDNPFFAISDFHGNFVIS
                     NVPVGEWEFQIWQERVRQLDLPQAPRGRLVVNVKPGVNDLGVIKVRPEQLNVV"
     gene            6552..7706
                     /locus_tag="PLD05_03260"
     CDS             6552..7706
                     /locus_tag="PLD05_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOP76479.1"
                     /translation="MGRHLSLSVIVAVLGILTCSCSPSPQFRLNTEGRDPATISPAQR
                     EAISSLLTDFFGTPDQPTVPRGVTLSLDDITKAAGPVGRDLRKVERGLYRKYCAACHG
                     ITGDGAGPFALFFDPYPRDFRKGVFKYTSTVLGAKPTTEDLEKILRNGMPGTGMPSYK
                     LLPDRDIRRLVEYVKYLSMRGETELYLLRLVLDENELLPLNKQSVLEDGVLAVAESWQ
                     LPQSDPDAWVVKPRPPILTTDGLKAAVERGRELYRDRRAKCATCHGPEGRGDGEQWDL
                     YDDWNTPKKGVTEQQTRDLARRFTLPIQRLRPRNFHQGIFHGGDAPEDIYLRIYLGIK
                     GTPMPAAGPAPGVPGVLTPDEIWDLTFYVLSLAGKLPQEITKNPSDGASP"
     gene            7722..8705
                     /locus_tag="PLD05_03265"
     CDS             7722..8705
                     /locus_tag="PLD05_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOP76480.1"
                     /translation="MKAPDSSINVQKTFSLPVTNWTCRLVWTLWGVALVLMALGHTVT
                     TYGAGMVIPDWPTTYGHLPFLLPPKEWFEHWDIFLAYSHRLVAIISGLLSLVVAAAFW
                     RTRQNLHKWLATAAVFLVCLQVALGGLRVLTGDLLLVTIHACTGPVYFGLVTVLAVIA
                     GSHRKRPSLDEPSPVKTRKRASVVSIFVASLAYLQLVLIAQLRHVSPQLIPTAPLVIV
                     WAQVIIWGILGAVILGICLFLPGSCRAALGRMGLWLISGLYMVNVLLGILAWIVNFNL
                     PPRFAHGVLTVEYTVVQAGAAQAVLTTAFVLVTSLLFATSLVLVVSNWLRE"
     gene            8982..10028
                     /locus_tag="PLD05_03270"
     CDS             8982..10028
                     /locus_tag="PLD05_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP76481.1"
                     /translation="MRDDQWQALLELVSQGSTVPRIGLLVDSPWVPGWYGITIREYLV
                     SDRLWLQANLKAVEEFPDVWFLPGFWSEYGMCTEPSAFGGRCRFGVNDFPHIEPMMRS
                     WEEVRSLTPPDCTRDGLLPFVIERLKLTEDEMGKRGHRVRFAVSRGPLNIASYLLGQS
                     EFLLGLKAEKELAHQLLRMVTDFIKQWVRYQKEVFPSIDGLLILDDLIGFIGPKDFEE
                     FVIPYFTEIFAAINVPVRMLHNDAYGLITARHLEKMGANIFHFSCDHTFQQVRELCGP
                     NVVLMGNIKPRDVLALGTVDDLRREIQEAWHTAGDRTRILWSAGGAVPPNVSSEKIRV
                     FCEELRKLAGASSV"
     gene            10137..12275
                     /locus_tag="PLD05_03275"
     CDS             10137..12275
                     /locus_tag="PLD05_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76482.1"
                     /translation="MGWVLIGGAGQAFCQMIGSSIGPEYHLSNDVRVNEVETAVRGKL
                     AQIRAAMEGGEALSAVEMIREVINQSGTRLVNITGELKGVPPRFVSVREYCQLLLTRM
                     PPDALASYRRQVDPTCRVWLDSGLRDRDPKPLQRLVDWELASSFADTALWFLGEWAIQ
                     DGNPALARSLWRQLLPTGRYLWEMPTWRAVPDSDYSAAAVRARLILCTILEGRLDEAR
                     QELTEFATLHGGERGRFAGQEIEYTVALQRLLEEAAHWPPSRESSDWPTLARSFERAA
                     ETAGPFDPIRLAWRAKLPPLPKVHVTLWSDTPSPEMIREKTRPLPYHPIVKQGRVFVP
                     VADGILGYELATGKPLWGASDPRIFASDLFAGREILVPVNTIGTLNWSATTDDHYLFA
                     RVGSPVTIMPTLLTDAIAQRSALVCLACDEEGKLRWQCEPPDQTWAFDGSPVVIEDRV
                     YAVMRRSDVPSHLYLACLSADQGNLLWRQFLCAANTPGRGFLYEMTHILPTVGEGMIY
                     LSTNMGAVAAVSATTGDIRWVTCYPRIDQGDLSTYEGYRYREPNPAVYWNGLLFLAPT
                     DSRQIFALDAFSGMIVWVCQAPTERIRHLLGVVGNRLIATGDRVYWIEATGPKAGQVV
                     ALWPEGAESLGYGRGLIAEEALYWPTLDAIYIIDALGAQPRKIFPLRPWHCEGGNLLW
                     ADGYLLVASSTELAVFQTSRPHVKTEIRSP"
     gene            12599..14083
                     /locus_tag="PLD05_03280"
     CDS             12599..14083
                     /locus_tag="PLD05_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP76483.1"
                     /translation="MPDGFQPGQGDGLEIAKTADRGWALHVSIPAGAPGHRNIRYMIP
                     ADRVRGKRLKVSANIKAQGISQPPNPWNGIKCMLQIVSPGETQWPQQNMPGGDFDWRR
                     ASFLAVIPDDVERVFLILGLENVSGDAWFDDVVIEDLGARKVPAAATSQPVFMGHRLP
                     RLRGAMIGPRVTDEDLLEFGQVWKANHVRWQLIWGGFPRSPADSASLKEYRDWLETAL
                     RRLDEALPVCRKAGILVTVDLHTPPGGRDSSSACRMFQVREFQEAFIQIWEEIARRYK
                     DSNVVWGYDLVNEPVEGAIPAGLLNWQELATETARRVRAIDDHHAIIIEPAPWGSPSS
                     LDLLEPLDVPGVVYSVHMYIPHSFTHQGVYNNPMGIVYPGMIDGRWYDRETLKKALSP
                     VVKFQRDFNVHIYIGEFSAIRWAPGNSAYQYLKDCIDIFEENGWDWAYHAFREWDGWS
                     VEHGPDPQDRRRTPELTDRAKLLRSWYSKNVAPSFSATPETNPK"
     gene            14164..15198
                     /locus_tag="PLD05_03285"
     CDS             14164..15198
                     /locus_tag="PLD05_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP76484.1"
                     /translation="MSDDDRKSLGFLAEAYQKLCAEMGKVIVGQETVIEQLMIGLFAR
                     GHCLLVGVPGLAKTLMIRTLASCLSLRFNRIQFTPDLMPSDITGTEVIQEDKATGVRE
                     LRFLPGPIFANLVLADEINRTPPKTQAALLEAMQEYQVTVGGKRHPLEPPFYVLATQN
                     PIEQEGTYPLPEAQLDRFMMNIQIDYPTEDEEMDIVLRTTSDISMTVESVLTGEDVLK
                     LQRVVRRIAVPEHVARYAVKITRWSRPQVPDGPKFVKDYVTWGGGPRATQCLVLAAKA
                     RAALHARPAIDIEDIRAVVLPVLRHRIILSFNAVAEGLKPDDIICRLLDSIGNPEDSL
                     IARGILRKLS"
     gene            15173..16078
                     /locus_tag="PLD05_03290"
     CDS             15173..16078
                     /locus_tag="PLD05_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP76485.1"
                     /translation="MGYSASFLEPQVLAGINGLVLRARGIVEGHVTGLHRSPFHGFSV
                     EFAEHREYSPGDDLRYVDWKVFGRTDKFYIKQFEQETNLSCYLLVDSSESMKFQSKGS
                     PLSKWAYAQCAAAALAYILLNQQDRVGLTIFSEKVLTHLKPEAGMPQWSNIISVLEQF
                     QPLGKTGLGGVLSEIAERAKQRGMVIVLSDFFTDLSEVEKGLKHLRYQRHDVIVFQIV
                     DRQEVTFDIEKPVVLRGLEGGATLAVDPAWLRRAYQTRFRQFCLQLQKICHDSGVDYV
                     FVETVNRFDKVLREFVSFRARCGRR"
     gene            16281..18515
                     /locus_tag="PLD05_03295"
     CDS             16281..18515
                     /locus_tag="PLD05_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOP76486.1"
                     /translation="MLQGGLVTLGFAFLQPAMLGWAVAALIPILIHLWRRHRYHDLEW
                     AAADFVLAALRTERRQILLRQYLLLLLRVLGILLVVFAAARPTFQKPMVAQTYHETTG
                     WVFVIDDSLSMQYTRDDGTLFEAALERIRNIVQSTPDGDAFLLLRGATPVRWAVRQAA
                     YNKGDFLAELNRLRCRDTAADWRTALQFVEQRLRELTQEEPRLGRFVVVLVTDGQETN
                     WRKLQDTPRASETSKAEDDKGADGNGQITNLAEFFVLLLGVEQPSNTAITELNAYPAN
                     VIVGQETAFSGSVTHYGPSETRPKRLEWFIDDKKIADQVLNWTSATEAFFSWTYQFDT
                     PGDHVVMVQLSADQLPADNQRRVVVSVKQERSVLCVDGRPSPLPFQGATGYLRVALGV
                     QRAASGRVSQRIDIIPEGRLAEVRLEEYESVFLCDVSRLTLGEAQALREFVESGGGLI
                     IFLGPRVNIQAYHESLGKSAFGSVNLLPGQLREPVSIQKSEIDPLGFRHPIVQVFQGR
                     WASSLLMVPIRTYVPLQLSREEDVEVALRLTNGDPLIVTESIGLGRVVVVTTSADLSW
                     TSLPLWTSYVPLIQEIQNYATSGRTASQTVVAGEAIGQLLPRSWQIDRHRLSLEPPWG
                     ERVEESLQLTGQYLRWSYANTEHSGIYRAEARLKSGNTQTTVFVANPPVAESDIRPWN
                     FVGFVERVLPQAKYVSELESLRITPTNVSSALRASPLSRWLLCFVLVVLLGELYLGRH
                     TPGT"
     gene            18577..>19327
                     /locus_tag="PLD05_03300"
     CDS             18577..>19327
                     /locus_tag="PLD05_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOP76487.1"
                     /translation="MSDWINRLGHTLMPHRFTDGTSQSVRVAGDWPWPVWITIVLIAL
                     IFFLIYRFYKKTRGDLSWKQRHLLFALRCITSAIVFLMLAQWILVTEQLGMPILAIIL
                     DDSLSMRIRDVDRQPKQGGEKANTGDPFDRERWNQAVDVLCGGPSPLLDELAQDYRLK
                     VYLLDGKTELKGPVSDIASTLRVMKPERATTPLGKAIHEVIDQLRGQPPVGVIVLTDG
                     VVNEGPGLEDAAKRASQELVPLYFVGFGAEKG"
     assembly_gap    19328..19427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19428..20744
                     /locus_tag="PLD05_03305"
     CDS             <19428..20744
                     /locus_tag="PLD05_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76488.1"
                     /translation="SRRILVKKDTIRILMVEEVPRFEYRFLRNALIREPGFDLTTVLL
                     GADPEYVEEMKGAMLAVPTSRDELWSYDVIILGDVAPNRVSPSTLEAVAAFVEEKDPP
                     GGLIVVGGPHAMPHEYNQSPLEKVLPCAVGVSPANIGQPKTAAHRLAVTREGLVMPWL
                     AFGRSPTETERVWHSLPPVYWLQSVIELKPGARVLVESREENQQEGRGEPVVVFQYVG
                     AGKVLFHATDETWRWRRGAGLAAYRHYWIEAVRFMARGKLESARTPRLVTDRSEYQAG
                     DSVHLMLECPKPAELPLRNGSVAIWLEYEGRTRREVTLQRHPLRGDLFEATLQNLAPG
                     QYYAWVPITSGIVPATGFAVVPPLQEWEATQMARKEMETAARLSGGGFYLPSEIRRLR
                     DDLPHGRGALIEMLPPRPLWNSPMVLLLLVLTLSLEWLLRKRYGLL"
     gene            20785..24615
                     /locus_tag="PLD05_03310"
     CDS             20785..24615
                     /locus_tag="PLD05_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76489.1"
                     /translation="MTPKFARHVETLRRRLRHRLVLRAWSWTILVFIAAVFSLGALDY
                     VLRFQERGLRFLATGTLLATTVWCFFRIVKPAMTLCIDRVALARWLEKFQGGAPISLA
                     AACDLVSLPPDDLRWGSPALRSMAIEQAEAHWTVPPWREVIERGPALWPSYLAAAALV
                     VAFSLLLVFPVVAGTALIRLVNPLSDLSYPQTTHLVVVHYPERLSRGDTFFLQVTEER
                     GQLPDEVRIAIRPLDGESHRAVNQLMKKVRNQFVFRQENVRTSFQFLITGGDDRSQPW
                     RRVEVVDPPQVSEWKVWLKPPEYTGWPPEEARGPLRALAGTEVQLSGRANRPLKRVVV
                     DCGPQQSFQGDVLPDQYSFRVPGGKNHRPEQERWILQTSGYYRIRLEAADGTFSVNEP
                     RWEVRVYEDAPPTVVLESGQRLLVMTPDGRLPLRVSARDDLAIRQINLVSRDSSGNSL
                     GPTIPLYQGPPFPPTQIRMAELETHADSVEKEYLFRLPVSAGNTGLTFRVVASVEDYK
                     GQASESEPLEIRVISPDEFNQRLASEEEKLLQQLAQLYESFVKISRDWERLSEKLRAE
                     KRLSRTEANQLLTLDTLERQAFRRIADPGLGILYELQDLAGMAEYQGVTQDRVERLKK
                     FRTDLEASWKQHGVPAESQLGMIANWARNLVERLLGDEQSLSIEKEWADVAQNADQFS
                     LHQAAWEKALEGLLQNVSGIRLVNTLRRELEKLLTEQLELIQQTRDLGQQTLGRALQD
                     LTPNEQDALTRIARRQTLLATKLEELVAKVLGGLQQSGNQDVTRQGFRPHEGSMSERE
                     DDLTIQSSQMVSQQGAVSQQSFEKPKGDAVPKEPQLIALIIDRINGHGLTALMRQAGL
                     AIEQNRLSEGLTLEEQSAEILRELIDLLDGKREDAGRRQQDLAEQENELRNLLRAQQD
                     LIRKFAEARQTAVEEALSTFIREQSKIEQRTRELADQMAPPLKRVAEPLLKEAIRRMQ
                     DAQTSARQKDVGKTEQNATLAQGLMEQALEAVGQERITQERNKAYDRWHEFVRRINGI
                     ISEQRNLLRKTQSLDIEKRKSQTPERLESWKKTVLELGRAQDALGQQVAQLERDIREA
                     PVIAAILTRVEEATRRAAKYLVGLECGVSTQEAQRMALSDLQLIARAIQGESSQVPPS
                     SAEQGTQPNAGDDQGPQSPRLVFLAPQIKLLRDLQASINQRTESLEKRRSQPAENPAV
                     LEQESERLSHEQAMVLNLVMKLRDDLLESASGEESRVTGPAREGAETPPATERQPNLP
                     KPGQPGSVRDLFNP"
     gene            24879..>25294
                     /locus_tag="PLD05_03315"
     CDS             24879..>25294
                     /locus_tag="PLD05_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76490.1"
                     /translation="MAATSEDSDPLVLVLELMKESQVRLARKDTGEQTRQIQSKIVGV
                     FDALLNQAMQSAQPQPSAGTTQNERQEPEEKTQTTQAQPRQETEAGSQRPTQQSGISQ
                     RGELNEVLQKVWGELPERERNAVLQHAAEAIVPKYRA"
     assembly_gap    25295..25394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25395..26311
                     /locus_tag="PLD05_03320"
     CDS             <25395..26311
                     /locus_tag="PLD05_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP76491.1"
                     /translation="AACIERGLQYLVSRQHVDGSFGSGAYRGNIAVTALAGMALMCEG
                     SLPNRGRFGEEVNRVLDYVLSNCHENGLILYVPSASRGPMYEHGFATLFLAECYGSSP
                     RDDLREKLSRAVRLIVTTQNNEGGWRYLPQPLDADISVTICQIMALRAARNAGIHVPK
                     ETIDRCVDYVKKCQNPDGGFRYMLIPGESAFARSAAGVVALYSAGIYEGPEIERGIEY
                     LSRFIPEPGVIRQEPYYFYGHYYCAQAMWQRGGQLWQRYYAGIRDELIAKQQPDGSWS
                     DPISPEFGTAMALLVLQIPNHYLPIFER"
     gene            26458..27654
                     /locus_tag="PLD05_03325"
     CDS             26458..27654
                     /locus_tag="PLD05_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOP76492.1"
                     /translation="MAGLSKCASQELLLTTGTIDEAKLMAVEGDSIVFQTKDGQRRIP
                     RDEIVCWGVLCEPRGGEWLCLHNGSLIAGKWLGLANGRLTWRNDYLGEFSVTVDQVAG
                     MIFRLPSSFIEKDLLIAQILDRTKGGYLLLANGDQLDGALLRRQDNGDLLVTTRFGER
                     TFSVEQLRGVSLRRVSAATQGPIDCAVGLKDGSRIIICKFTRGTEDPASKDLAPALGD
                     REIQGVLSNLLQNRIEDICFYQPFGNVVYLSDLAPETFIAVPFLEYVRPLKMDRNALG
                     GWLRTEEGAFLKGIGTYSACRVRYNLQGRFSRFAAEVAIDACTQGKGSVMCHVLVDGE
                     RKVSVGPIRGGDRPVPVVVDVTGAASLDLVVDFGERADEQDYVNWLNARLIPATTSLT
                     QEKGGD"
     gene            28010..28333
                     /gene="groES"
                     /locus_tag="PLD05_03330"
     CDS             28010..28333
                     /gene="groES"
                     /locus_tag="PLD05_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOP76493.1"
                     /translation="MAKKKGDKEESKLQLQPLADRVVVEREESEEKTPGGIVLPDTAK
                     KKPSRGVVVCVGPGRMLEDGTRAPMQVKVGDRVLFSAYAGDDFKIGDQELVLLREEDI
                     LAVIE"
     gene            28389..30002
                     /gene="groL"
                     /locus_tag="PLD05_03335"
     CDS             28389..30002
                     /gene="groL"
                     /locus_tag="PLD05_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOP76494.1"
                     /translation="MAKLIAYDQEARDALRRGVAKLAQAVKVTLGPRGRNVIIQKSFG
                     SPTVTKDGVTVAKEIDLEDVYENMGARMVREVASKTSDIAGDGTTTATVLAEAIFSEG
                     LKAVVSGINPIGLKQGIEKAVEDIVAKLKAMSIPIKTSKEMAQVAAIAANNDMAIGEL
                     LAQAMDKVGKDGVITVEESKTMETKVEFVEGMQFDRGYLSPYFVTDPQKMECVLEDCY
                     ILIHEKKISNARELVPLLEAVVNAGKPLLVIAEDVDGDALATLVINRLRGTLRCCAVK
                     APGYGDRRKAMLEDIAILTGGVALFESLGRKLEHVTLAELGRAKKVVVDKDNTTIIEG
                     AGKTEEIKGRIEQIRREIEKTTSDYDREKLEERLAKLAGGVAKLNVGAATEAEMKEKK
                     ARVEDALHATRAAVEEGILPGGGVALLRAAMQCKPDGLTTDEKVGYEIVLRACRSPIM
                     QIASNAGQDGSIVCEKVSEMKGNMGFNALTGQYEDLVKAGVIDPTKVVRVALQNAASV
                     ATLMLTSDALVAEKPKEEKSGGGHTPPEY"
     gene            30369..32267
                     /locus_tag="PLD05_03340"
     CDS             30369..32267
                     /locus_tag="PLD05_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76495.1"
                     /translation="MQPPLECRPLQIVHGLTPQRVVADSVAQILQQPRAQPISDEGMA
                     YYLRRGRGGIVCNVAFQDYLRSEANWQTLIEGVELCARLKMPVWIYDEKGYPSGAAGG
                     LVLEGNPEFEALVLAWEPRQTPAFFLRRAFEHTHAANNYHAVRRYINLMDDRATAKFI
                     QVTHEAYYKRLARHFGSTIVAFFTDEPSLMAVDLGAIPEPARSRVPIEDAPDPNVKPL
                     PTVPWCYDLENVYRSKWGEDLRAQQKSLFVGNDPEDRRIRRQFWSIVAELVADRYFGA
                     LRRWCQGHHVASSGHNLYEESILHHVPLYGNGLKAIAQMDVPGLDMLSSNPEVVIHSG
                     WLTAAMPVSGALLTGCRRVMTEVSDFSQKMSGAGPVGLPEMMATAAWQAAWGVTEFTL
                     YYNPEDRSVEENWQYGQFVGRLNAILREAMPRRRVALYYPIYDLWAEYLPVAGPLHQS
                     VRSEKVQQLIGSFMRLGQTMQRRQIPFVLIDHEYLQRSRVSSDGRLCVGNLELESVVM
                     PAGVELPHEAQAVIEQFQQAGGRVIRDATDAPLTAAKLVEAIRPLYHIEPANDRIVFG
                     EFEREGRTILILANVSREPYKGVLRAKQHKTWTALDPITGEIRAVTATEQGIPVHLDG
                     YKAILISQ"
     gene            complement(32403..32798)
                     /locus_tag="PLD05_03345"
     CDS             complement(32403..32798)
                     /locus_tag="PLD05_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOP76496.1"
                     /translation="MRHQHGGKRMAQPQPEHLRGDRYYSPPVDIVEKGDEILLIADVP
                     GTKSEDIDIQFEDGMLTVHAKVNPRYPEGVEFLQQEYGVGDYLRTFQVSEAIDASKIS
                     AEYENGVLTLHLPKAASLRRRKIEVTAKG"
     gene            complement(32846..33286)
                     /locus_tag="PLD05_03350"
     CDS             complement(32846..33286)
                     /locus_tag="PLD05_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOP76497.1"
                     /translation="MAVPPFGPPSLDQLRREVDRLFNNFLSELPEAGLGLTSRNRIPV
                     NVWETPEAFVVEAEVPGVTTDQVDISVVGSELTLRVNRPQPANEGVTYHRRERQTGEC
                     SRVIRLPGEFDVDRIQADLQNGVLTITLPKAETVRPRKIQVNVR"
     gene            complement(33550..>33897)
                     /locus_tag="PLD05_03355"
     CDS             complement(33550..>33897)
                     /locus_tag="PLD05_03355"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOP76498.1"
                     /translation="GETLQSESEDGGKTWSVPHPIGVWGLPSHLLRMADGRLLMSYGH
                     RRPPFGCQARISEDEGRTWSEPIVIYGDGAGGDLGYPSTVQLAGGDFVTIWYELMSGS
                     PFAVLRQARWRLA"
BASE COUNT         7377 a         8584 c         9876 g         7860 t
ORIGIN      
        1 gttacacttt tggcgggtga gaaaagacgg gcggatcagc ggacccctgt gaggcggact
       61 gcccgtgaac caagccgcgg tccgaaaagc cggcctcttg ggtcgcgaat catcctgacc
      121 gagacatcat gaacctgtta ccgattgtga tctgttattt gctgcttgcg ctgagtagca
      181 caattgcctt ggcggttatt cttcgtcggc gatcgccgcc acgccggcga gtatcggact
      241 tcgtcgatat cgcggcgctg ggcagctacg ctgttttggg agtgcttggt gcgctgtcaa
      301 cggcggtggg acatcctccg ggtttaccgt ccgccgtgaa agacgcttta aattggctgc
      361 tgactccggc cacggtaatg accttatggc tgctggccat ggtcatcctc ttgtggagac
      421 caagtatttt ctcttcgccc tggatctttt ggcacgctgc cacgtggggt agtctgtggt
      481 ttttactgtc gatgacggac agccactttg cggcactcgt tctcaagccg gatcacattg
      541 ccgtggtctt tttggtcgca ctgacaggct tcttcacctg gctagggctg cggcaaagtg
      601 ttctcaatga ccaacggtta gatcgtggcc ggaaaccacg agaggcgaca aaaccggaac
      661 aaacacgggt ttttgtgtgg cctgacctcg tgtatatcga actcatcgcc atcctgggga
      721 tgacatgcgt tttgggggtg tggtctttgg ccgtcccggc acccttggaa gccccggcgg
      781 accctgcgtg gacgcccaat cctgccaaag ccccctggta ttttgtgggg cttcaggaat
      841 tgctggtcta ttttgagccg tggtttgcgg gcgtcattgt gccggttatc atcacagtgg
      901 gcttgtgttt gattccctat ctgcggcgtc gcgatcagcg atcagggtat tactcgctga
      961 agggccgccg cttttccgtg gcgatctttc ttttcggtct tggtctgtgg gtgttgctga
     1021 ttctgattgg aacatttttt cgcggacccg actggcgttt gtatggactg tacgaatcgc
     1081 ggatggtgag gcgatttgac gaacttcgcg cggtcccact ctcctttctc gtttggcgtc
     1141 ttgtgttgcg cactgccgac acatcacttg agatgactga tattccaagc tcgccatttc
     1201 tgcgggaact tcccggcctt gtaatcattg cctttctttt ttttgttttt cccttttttg
     1261 ccaagcacac gtttggacgg aaatgggctg accgacttgg caagacagga tattggacag
     1321 tggcaatcct tcttgcgatg atgaccttga tcccgatcaa gatgtatgcg aactggttat
     1381 ttgacatcaa ttacttcatt tatctccccg aaattgggct gagtctctaa agcgagacga
     1441 cgttgaagcg ttcatgcctg cggctagggc gggtcagttt tcgaaaacaa cggcagtcat
     1501 ggatagaaaa gtcgatcatc attatccgat ttggctcctc catgcgattt gccttttggc
     1561 gtgcgctgcg atcattgtga ttggcgtgat cctggcaact agggagcaac ggcaagcgtg
     1621 gcggggcttt cagcgccatg gcttacggat tttggagtcc gccatggctc atgatgcgaa
     1681 gagcggacga atgactcccc aaggcgagtg catttcgttc ggcacgcaaa agctgcctct
     1741 agcggtgatg gatgatgaac ctctagccat gacgatagga cggatgcatg aacttatttt
     1801 gccggattta ccccatcggc tcggtccggt cgagtctcca cggagagaac gttgcatgac
     1861 gtgtcacgtt ttgatcgatt ggacgggcga cactctgtcg gtctctgatt cttcttcaca
     1921 agatcgagga cccatctgga ccaggcctac ccgagtctca gaaccgatat cggaggtttt
     1981 tcccctcgag acgattttgg aaatagtgct gcctgtcgtc atcaccggca aggcgggcga
     2041 tggggtaccc agtaacagag aggagaaggc tgatacgagc ttgacttttg gattctcctg
     2101 ggtcgagccg ggacttttga agggagacga accgcgaatt ggcacggtcc tgcgtgggag
     2161 tcccgcggat tcggcagggc ttcggccggg ggacctgatt tgtgccattg gcaactcccc
     2221 cgtggagacc aaagaccagg cggagaccct cttatccgag tctgtgaggc aactcaaagg
     2281 cctttcccag gatctaaaag gagatttcgt caagacctat tatcctgcgc ctttcggcag
     2341 cggggagagt gtcccgggga ttgctatcaa agttcgccgg gggcttccgc aaccatgggc
     2401 tggccacccc cacccagagc tttatgtggc ggagcgaagt ccccatccgg tcaggcgatt
     2461 tggttgcacc atttgccacg acggacaggg acttgcccta gactaccggc aagcggaaca
     2521 tccttcgccg attgtggagg ggagctctct tggaaaacgg tgctatggtt acgccatcaa
     2581 cgttcgggag tccatcgaac cgatgatcct caccgaattg gcggagagtc gttgtctgcg
     2641 ctgtcacgac agggctttcg atctcgccga aaccgatatc cgatgggaac caattgcgaa
     2701 gcacctagtc gagggacgac gccttgtcga gaagtttggg tgtttttcct gtcacgaaat
     2761 gcaacgtgat tcagcaccgg gtgaaccgca attaggcccg gtctcgcctg cgacagagat
     2821 agccgaggag cttttagcgc tgtcttccac tccacaagag cttaaggaga tagcagttcg
     2881 cttcctggag acgccaaacg ataaggaact cctagaacgg ctccggaaag agacgacgcg
     2941 gtggcaagat ctcttggaga gtgacaccag gaggtttcca gaggatcgag agatctttgt
     3001 gacaagacag caggtgcggc aactcgtcgg ggcattgcag gcggccaacg tgatctccgg
     3061 aggctggtcg aaagtcgggc caagtctgcg atatgtcgct tcaaaacttg cccctaaaac
     3121 gatcgaacgc gtaatcagcg agccgcagag cgtccgcccc gagagccgaa tgccgcagat
     3181 ctttggtctc acagaccatt taagtgaggg gccagctcag caccgaaaac agttcgagca
     3241 ggttgagatc gccggaatcg tcgcttactt ggctggccaa agtcacccgc tgccgtctaa
     3301 tcgcacagag acgcgcgcac ctccccaaga caagttgggt tctcggagcc tggcagacgc
     3361 ggggcgaatc ctttttgcca cacaaggttg cctggcctgt catcgccacc gcgattttcc
     3421 cgaaggcact tcggacgttg gtcccgactt gtcggacctg ggacagcgtc ttgtgtgcga
     3481 caccggtcgg gagtggctgc ggctttggtt gacttcgcca cagacacttt ccccttctac
     3541 ccggatgccg caaccacaat ttcgggacga cgcctggatt tatcgtgtct ttgaaccggc
     3601 cgagtccaag ccacgccggg tgacgagctc cgaggtcatc gacgctctag tggctttctt
     3661 gctatcgggc gagtctagta cgaatggttc tcaacagccg gaaaaactgc ctgttaacgc
     3721 tgaatggctc gatgaggtcg tcttggaata tctttcgtcc gacttgccga gggagaaggc
     3781 ccaaggaatt gtgaggaacg gtgccaccgt ggcggacctt ggcaagtact cgctccaggt
     3841 tgccttggaa ttgacagaac ttttgggtga accaacgagc gacaagaaac tgcgatacgt
     3901 gggacggaag gccatcgctc gatatggatg ctatggctgt cacaatattt ccggttttga
     3961 gggttggccg cccatcggac cgagtttgag tcagttcgga tccaagccag tggcgctctt
     4021 ggactttggg gcggtggcag aaggattgct ctctacgcca gaaggacggc acatcgtaca
     4081 aaggcgaaaa acacccttgg aagatcgcct ctcttctctc gacttcctgg gtcgcgaaac
     4141 gtggttgtgg ctgaagctca tttctcctcg ggcatttgat tttaaggcta tggaaggcaa
     4201 gccgatcctg caacattcgc ggatgggtcg atttccactt tccggcgagg aacgccaggc
     4261 ggtcatgacg tttattctgg gacttgaagg gcgaccagtg cccgcgaagt atgaccctta
     4321 ttccgcgtcc cgaaaggcac tcgccttggg tcgcgagatc atcgaacgcc gggcgtgcga
     4381 tcggtgccat gccctccgtc cggagcaatg gttctttcga ttttcggaag aagatgtagt
     4441 gcgggaaatg ggcggggatt atgacccaat tggaaggcaa acagaagccg aaaaagccag
     4501 tgccatgcca ccgcggcgcc ccctgggcta cgccctagct atgggccaag ccgagcgtga
     4561 cgcctccggg caaatcatcg aagaccgtga cgattttgac aatccactgt attttttcat
     4621 gccctggcgg ccagttgcct ttggtgctca catttggccg gtgggtgaag cgagtatccc
     4681 tattgcctcg acgcaattac tagggaagcg agaccaggat ggaggtgcgt ttgccttcat
     4741 gttatatccc acggtggtct ctctcatcca acaaatccaa ttgtccatgg gactaaaaga
     4801 aacgatggga tgcctgccgc ctccgcttta ttacgaaggc gttatggttc agccttcgtg
     4861 gttatctcag tatttgaagg aaccttatcc catccgaccg gccattccgt tggctatgcc
     4921 acgctattgg ttgacgaatg aggaggtgca aatccttgtt gactatttta gcgcgctggc
     4981 ggaaaagacc ggtgagtcgg atacaagttg gaaaacgacg gaaactcaat tgattcttcg
     5041 tgagcagcag tggccagggc ggctcgatgc ggcctggcac ttgatccatg atacgaaaac
     5101 gtattgtgcg aaatgtcata tttcttcgga caatatggaa gatcgcctgg ccctgccgac
     5161 agaggccccg cgacttgatg gagtttatcg acgcttgcaa cacgacttta tccgggcatg
     5221 ggtagtccaa ccgaaggcaa ttcggcccta cacggccatg ccggtcaact ttcccgaaaa
     5281 cggtatcccg ctggatcgaa gcgtcttcga tgctgacagt cgtgttcaac tggaagctgt
     5341 ggtggacctc ctccagaact acgattggta cctcaaacaa cgtgtgaggc aggagttggg
     5401 tcccgcgcaa tctcagtgaa cagcggcggc ttgaatttcc caggattgct ttgtgggtcg
     5461 aaaaacagac gcgatgtgct tttcagtcgg acaattatgt acaatggact gcacacggga
     5521 agtcctgcta tcctcatgct gattcccacc gatttgttgc ctaccgggca agaaaaaaga
     5581 ttgtttgttt ggtgattccc tcacttgcga tgagatcgta ctaatgtcgt atcagagaag
     5641 caaagatgat aaacaaattt cgaagcaccc gtcgggcatt tcttaagagc agtttgtcac
     5701 tggctgtcgg gacagccgcc gtagggttaa cgcgaaaagc cactttcggg ataactgagt
     5761 ccgctcgtgg cgaggtccgt ggccggtttg tctatgatgg ccaaccgccc gagcgaaaaa
     5821 agctgaaagt cgataaagat gtggactgtt gcggaaagta tgacatccgt gatgagtccc
     5881 tgatggtcgg tctggaaaat gggttggcga atgtgtttgt gtatgttcga acaaggggga
     5941 tccccatccc gccggatatg gctgaacaaa taccgaagag ggtagtgctc gacaaccacg
     6001 agtgcatttt caaaccgcat tgcatggcca tttggtaccc tgtccaggag ttttatattg
     6061 tcaactcgga cccgattgcc cagaatgtgg cgtttagccc gttgggagac ctgccggcta
     6121 acatcatcct tgcccccgcg ccgggtgata atatcgatgc agtctggagg ttccgccgaa
     6181 gtcagaccga gcctgtgctc attgtttgca attatcatcc ttgggaaagt gcctacattt
     6241 taccccggga taatccattt tttgctatta gcgatttcca cggtaacttt gttatctcga
     6301 atgtgccagt aggcgagtgg gaatttcaaa tttggcaaga gcgggtccgt caattggatt
     6361 taccacaggc cccccgaggc cgcctggtgg ttaacgtcaa accaggggtg aatgacctgg
     6421 gtgtgatcaa ggtcaggcca gagcagttga acgtcgtttg aggagaatgc ctcccaagac
     6481 ttggtggtgg cgttcgacgt ggctggtaaa tgtatgaacg cacatgacgg aggctaacct
     6541 ggatattaga tatggggaga catctgtcct tatccgtgat cgtcgctgta cttggaatac
     6601 tcacatgctc ctgctcgcct tccccacagt ttcgattgaa taccgagggg cgagatccgg
     6661 caacgatttc accggcccag cgagaagcca tctcgtcgct gctgacggat tttttcggta
     6721 caccagacca gccaaccgta ccgcgggggg tgacgttgtc attggatgac ataacgaagg
     6781 ctgcagggcc tgtgggccgt gaccttagaa aggtggagcg agggctttac cgaaaatatt
     6841 gcgcggcgtg tcacggaatt accggcgacg gtgcgggccc tttcgcgctt tttttcgacc
     6901 cttatccgcg cgattttcgc aaaggtgtgt tcaagtatac gtcaacggta ttgggcgcca
     6961 aaccgacgac ggaagactta gaaaagattc tgcgtaacgg catgccgggt acggggatgc
     7021 cctcctacaa actgttgcct gatcgtgaca tccgtcgcct ggtggagtat gtcaagtatc
     7081 tgagtatgcg aggggaaaca gagctctatc tcttacggct tgtgctcgac gaaaacgaac
     7141 tgttacccct caacaaacag tcagtcctcg aagacggggt gctggcggtg gccgaatcct
     7201 ggcaattgcc acaaagtgat cctgacgcgt gggtggtgaa acctcggcct cctatcttaa
     7261 cgaccgatgg tttgaaagcc gccgttgagc ggggccggga actgtatcga gaccgccggg
     7321 ccaaatgtgc gacatgtcat ggtccagagg ggcgtggtga tggcgagcaa tgggatcttt
     7381 acgacgactg gaacacaccc aaaaagggag taacagagca acagacccga gacttggcac
     7441 gccgtttcac tttgccaatt cagaggttgc gaccacggaa cttccaccaa gggattttcc
     7501 acgggggcga cgcacccgag gatatttact tgaggattta cttgggaatc aaaggtacgc
     7561 ccatgcctgc ggcagggccg gcgccaggtg ttccaggagt gttgacaccc gatgagattt
     7621 gggatttgac gttttacgtc ctttcgctgg cgggcaaatt acctcaagag attaccaaga
     7681 atccgtcgga tggcgcatca ccataacgtt gcgggtatgt gatgaaagca cctgatagct
     7741 cgatcaatgt tcaaaaaacg ttttcgttgc cggtcacaaa ctggacctgc cgtctggttt
     7801 ggaccctgtg gggtgttgcg ctggttttga tggcgctggg gcataccgtg actacttacg
     7861 gggccggcat ggtcatcccg gactggccca ccacctatgg acacctgcca tttctccttc
     7921 cgcccaaaga atggttcgag cattgggata tattccttgc ctacagccat cgactcgtgg
     7981 ccatcatcag tgggttactc tccctcgttg tggcggcggc tttttggagg actcgccaaa
     8041 atctccataa atggttagcc actgcggcgg tctttttagt gtgtctccaa gtcgctttgg
     8101 ggggattacg ggtcctcacg ggtgatctcc tcttggtcac catccacgcg tgtacaggac
     8161 cagtgtattt cggtcttgta acagttctcg ccgtcattgc cggttctcac aggaaacggc
     8221 cttcactcga tgagccgtcg ccggtcaaga cgcgcaaaag ggcatcggtg gtctcgatct
     8281 tcgtggcgag cttggcgtac ctccaactcg ttcttattgc ccaactccgc catgtgtcac
     8341 cccaactgat accaacggcg ccgttggtca ttgtgtgggc ccaggtgatc atttggggaa
     8401 ttctcggcgc tgtgattctg ggaatatgcc ttttcctgcc agggtcttgt cgtgctgccc
     8461 tgggacgaat gggcctgtgg ctcatttcgg ggctttatat ggtcaatgta ttgctgggga
     8521 ttctcgcgtg gattgtgaac tttaacctgc cgccacggtt tgcccatggg gtactcaccg
     8581 tcgaatacac ggtagtgcaa gctggtgcgg cacaggcagt gctaacaacg gcctttgttc
     8641 tggtcacttc tcttcttttt gccaccagcc ttgtcctagt tgtttcgaac tggctgcgtg
     8701 aatagctaac ctccgcataa aatggggctt cacacgaaga tttttcgtga caaaggccga
     8761 ggatgatgac gggcaaaaag tcggcacaga cggggggatg accggcactc tgtggttcgt
     8821 ctctggtgca ggaaacggtt gaatgacagc cggtgatcct gcggtatgag taagaagaga
     8881 ggattagaat cccggctcgg agcattccga agagatgtga gaaatagtat cataaaaagg
     8941 tagcttgcgt ttccttgggt cccagattaa ggagcgaacc agtgcgtgat gatcaatggc
     9001 aagcgctgtt ggaattggtt tcgcaggggt caacggttcc acgtatcggg ctccttgttg
     9061 actctccgtg ggtccccggc tggtatggaa tcaccattcg ggagtatctc gtcagcgacc
     9121 gcctttggtt gcaggccaat ttgaaggcag tggaagagtt tcccgatgtg tggtttctcc
     9181 ctgggttctg gtcggaatac gggatgtgca cggagccttc ggcgtttggt ggccgatgtc
     9241 gttttggtgt caacgacttc ccccatatcg aaccgatgat gaggtcttgg gaggaagttc
     9301 gaagtctcac gccgccggac tgcacacggg acgggctgct gccttttgtt attgagagat
     9361 tgaaattgac cgaagatgaa atgggcaaaa ggggccaccg tgtccgtttt gccgtcagcc
     9421 gaggaccgtt gaatattgcg tcctacctcc ttggccagtc ggagttcctc ttggggctga
     9481 aggctgaaaa ggagctcgcc catcaactgc tgcggatggt aacggacttc atcaaacagt
     9541 gggtgcggta ccaaaaagaa gtatttccca gcattgatgg attattgatc cttgatgatc
     9601 tcatcgggtt cattggtccg aaggactttg aggagtttgt catcccctac tttaccgaga
     9661 tattcgcggc cattaatgtc cccgtccgca tgctgcataa cgacgcctat ggtttgatca
     9721 cggcccgtca tcttgaaaag atgggggcca acatattcca tttttcgtgc gaccatacct
     9781 ttcagcaggt gcgggagctg tgcggtccca atgtggtctt gatggggaat atcaagcccc
     9841 gtgatgtcct agcccttggc accgtggacg atcttcggcg ggagattcag gaagcctggc
     9901 acacggccgg agaccggaca cgcattcttt ggtcagcggg tggggcagtg ccgccgaacg
     9961 tgtccagtga aaagatccgc gtattctgcg aagaattgag gaagcttgca ggtgccagtt
    10021 cggtgtaggt cgccaaaacg acaataaccc aagagggtac ctctgagcgt ccaacgggat
    10081 tgggcaaact gggtatgctg agacagcaat tcgctcattg gtggaaaacc tggctcatgg
    10141 ggtgggtatt gattggtggc gcgggccagg ctttctgcca aatgattggc agttccattg
    10201 gaccggagta tcacctttcg aacgatgtgc gtgtcaatga ggttgagacg gctgtccgcg
    10261 gtaagcttgc ccagattcgc gctgccatgg aaggtggtga ggccttaagt gctgtggaga
    10321 tgatccggga ggtgatcaac cagtcgggca ctcggcttgt caatatcacc ggtgaactga
    10381 agggggtacc gccgcggttt gtttcggtcc gggaatactg ccaactcctc ttgacgcgga
    10441 tgcctccgga tgcgttggcg agctaccgac gtcaggtcga tcctacgtgc cgggtttggt
    10501 tagacagtgg cttgcgcgat cgggacccga aaccactgca acgtttggtt gattgggagc
    10561 tggccagcag ttttgcggat acagccttgt ggtttcttgg ggaatgggcc attcaggacg
    10621 ggaatcccgc tctagcccga agtctttggc ggcaacttct ccctactggt cggtacttgt
    10681 gggaaatgcc cacctggcgt gccgtgccgg atagcgacta ttcggcggca gccgtgcggg
    10741 ctagattgat cctctgtaca atcctggaag gtcgccttga tgaagcgcgc caggagctta
    10801 ccgagttcgc tacgctccat gggggcgagc gcggccggtt tgctggccaa gagatcgaat
    10861 atacggttgc cctccaaagg ctcttggaag aggcagctca ttggcctccg tcacgtgaga
    10921 gttcggattg gccgacattg gctcgaagtt tcgaacgggc agcagaaacg gccgggccgt
    10981 tcgatccaat tcgccttgcg tggcgggcaa aactgcctcc gcttcccaaa gttcatgtca
    11041 ccctttggtc cgacacacct tctcccgaaa tgattcggga aaagacccgc cctttgcctt
    11101 atcatcccat tgtcaaacag ggtagggtat ttgtcccagt tgcagacgga atcctgggct
    11161 atgagcttgc taccggcaag ccgctttggg gggcaagcga ccctcgcatt tttgccagcg
    11221 atcttttcgc gggtcgggaa atccttgttc cggtgaacac cattgggact ctgaattgga
    11281 gcgctacgac ggatgatcac tacctattcg cccgagtggg ttcgcccgtg acgatcatgc
    11341 ccacacttct taccgatgct attgcccaga ggagcgcgct cgtttgcttg gcctgcgatg
    11401 aggaagggaa gcttcgctgg caatgtgagc cgcctgatca gacttgggcc tttgatggtt
    11461 cgcctgttgt gattgaggac cgtgtttacg cggtcatgcg acggagtgac gttccgagtc
    11521 atctttactt ggcgtgtctc tccgcggatc aggggaattt attatggcgg caattccttt
    11581 gcgcggctaa tacgccaggg cgaggctttc tttatgagat gacacatatt ctgccgaccg
    11641 tcggggaagg gatgatctat ctctcaacga acatgggggc ggtggccgcg gtgtctgcca
    11701 cgacaggtga cattcgctgg gtcacatgct atccgaggat cgatcagggt gatttgagca
    11761 cctacgaggg ctatcgctac cgcgaaccaa atccggccgt ctattggaat gggctcttgt
    11821 tcctcgctcc caccgatagc cgacaaattt tcgccctcga tgcgttctcg ggaatgattg
    11881 tctgggtctg ccaggcgccc actgagcgta ttcggcatct tttgggtgtg gtgggaaatc
    11941 gactgattgc tacaggagat cgagtctatt ggatcgaggc caccggtccc aaagcaggac
    12001 aggttgtcgc gctttggcca gagggcgcgg aatccctggg atatggtcgg ggacttattg
    12061 cggaagaggc tttgtattgg ccaaccctgg atgcgattta cattatagac gccctcggtg
    12121 ctcagccgag aaaaatcttc ccgctacgcc cgtggcactg tgaagggggg aacctccttt
    12181 gggcggacgg gtatttgcta gtggcatcat ccactgagct agctgtattt caaacaagca
    12241 gacctcatgt caagaccgag attcgttccc cgtaaggttg tttgttaaga tgatggaaca
    12301 ggggtgtggg ttgaaaagaa aacgctcgta tcaaggcaag gacaatctta tgacttggaa
    12361 gaaaatcgtc ctaaactttt cgcgcggtcc agtaattctc cccgttctgg gagggatcaa
    12421 attacctcgc gcgactgttg ggcatcgaga attccgatta tgtcgctcct ggtctttcgt
    12481 ggttgttgtg gcagttctgg ttttatgggc ttgccgtggg ctggacgctg gggaagtttt
    12541 gtttcgatgg gactttgagc cccagggggt ggacagtgtt ggcgagcgga tatcgaccat
    12601 gcccgatggt tttcaaccag ggcaaggaga cggcttggag attgcaaaga cggccgaccg
    12661 ggggtgggcg ttacatgttt cgatccccgc tggagcgccg gggcatcgga atatccgata
    12721 tatgatcccg gcggatcgag ttcgtggaaa gcggttgaaa gtttctgcaa atatcaaggc
    12781 acaaggtatt agccaaccgc ccaatccgtg gaacggcatc aagtgcatgc ttcaaatcgt
    12841 ttcgccgggc gaaacgcaat ggccccaaca aaacatgccg ggaggtgact tcgattggcg
    12901 gcgtgccagt tttttggcag tcattccaga tgatgtggag cgggtttttc tgatcttagg
    12961 tcttgagaac gtatcgggcg acgcctggtt cgatgatgta gtcatcgagg acttgggggc
    13021 ccgaaaggta ccggcagccg cgacaagtca gcctgtcttc atgggacatc ggttgccgcg
    13081 attacgcggt gcaatgattg gaccgcgtgt tacagatgag gaccttcttg aatttggaca
    13141 ggtttggaag gccaatcacg tgcgttggca actgatttgg ggtggcttcc cacgaagccc
    13201 tgccgatagc gccagtttga aggagtaccg tgactggcta gagacggcgc ttcgccggtt
    13261 ggatgaggcg ttgcctgtct gccgcaaggc tgggattttg gtaaccgtgg atctgcatac
    13321 gccgcccggt ggcagggata gttcctccgc gtgccggatg tttcaggtac gggagtttca
    13381 ggaggcgttc atccagattt gggaggaaat cgcacgacgt tacaaggata gcaacgtggt
    13441 atggggatac gatttggtca atgagccggt cgagggagcc atcccggcgg gcctgctcaa
    13501 ttggcaggaa ctggctaccg aaacggcccg gcgtgttcga gccatcgacg atcaccacgc
    13561 cattatcatt gagccggcgc catggggaag tccgtcatcg cttgatttac tggagccgct
    13621 cgatgttcca ggggtcgtct atagcgtcca tatgtacatc ccgcatagct tcacccatca
    13681 gggtgtgtac aacaacccca tgggaatcgt ttatcccgga atgatcgatg ggcgctggta
    13741 cgaccgagaa acactcaaaa aggcactcag cccagttgtg aagtttcagc gggatttcaa
    13801 tgtgcacatc tatatcggcg aatttagtgc cattcggtgg gcccccggca acagtgctta
    13861 ccaatacctg aaagactgca ttgacatatt tgaagaaaac ggttgggatt gggcctacca
    13921 cgcatttcgt gagtgggatg gctggagcgt agaacatggg cccgatcccc aagaccgccg
    13981 tcgaacacct gagttaactg accgggcgaa actcttgcga agttggtaca gcaaaaacgt
    14041 ggcaccttcg ttttcagcca ctcccgagac aaaccccaag tgattggtta acggcacgta
    14101 ggtttccgca agatatgagt gcgctcacct tattggcgat ggacattcgg agattcacca
    14161 cgcatgtcag atgacgatcg taaatcgttg ggattcttgg cggaggctta ccaaaagcta
    14221 tgcgccgaaa tgggcaaggt tattgtgggc caggagaccg tcatcgagca attgatgatc
    14281 ggtctgtttg ctcgtggcca ttgcctgttg gttggtgttc cgggattggc caagacgttg
    14341 atgatccgta ccttggcatc gtgtttgtcg ctacgattca atcgtatcca atttacgccc
    14401 gacttaatgc cctcagatat tacgggcaca gaggtaatcc aggaggacaa ggccaccggg
    14461 gttagggaat tgcgatttct gccgggccct atttttgcca atttggtgct ggccgacgag
    14521 atcaatcgta cgcctcccaa gacccaggcg gcattgctcg aggcgatgca ggaatatcaa
    14581 gtcacggtgg ggggcaagcg tcacccctta gaaccgcctt tttatgtcct agcgactcaa
    14641 aatccgatcg agcaggaagg aacctatccc cttccagagg cccaactgga ccggttcatg
    14701 atgaatattc agatcgacta tcccacggaa gatgaggaaa tggatatcgt cctgcgtacc
    14761 acatcggaca tatccatgac ggtggagtcg gttttgacgg gcgaagatgt gctcaaactt
    14821 cagcgagtgg tgcgtcgcat cgcggtgccg gagcacgttg cccgatatgc cgtcaaaatc
    14881 acgcgctgga gtcgtccgca agttcctgat ggtccgaaat tcgtgaaaga ttatgtcacg
    14941 tggggcgggg ggccgcgggc cacgcagtgc ctggttttgg ccgccaaggc acgagccgcc
    15001 ttgcatgcgc gtcccgccat tgatatcgag gacatacggg ccgtggtcct accagtactg
    15061 cggcatcgga tcatcctaag tttcaatgcc gtggcagaag gtctgaaacc agacgatatc
    15121 atttgccgtc tcctggattc catcggtaac cctgaggatt ctttgatcgc ccgtgggata
    15181 ctccgcaagc tttcttgaac cacaagtctt ggctggcatc aacggacttg tccttcgtgc
    15241 ccgtggcata gttgaggggc acgtcacggg cttgcaccgt agtccgtttc atggattttc
    15301 ggtggaattt gccgaacacc gcgaatattc accgggcgat gaccttcgct atgtagattg
    15361 gaaggtcttt ggtcgaaccg acaagtttta catcaaacaa tttgaacagg agaccaacct
    15421 ctcgtgctat ctgctggtgg attccagcga gagcatgaag ttccaaagca aaggtagccc
    15481 actcagtaag tgggcgtatg cccagtgtgc tgcggctgct ttggcgtata tcttgctaaa
    15541 tcagcaagat cgggtggggc tgaccatctt ttctgagaag gttctcaccc acctcaagcc
    15601 cgaggccggc atgccccagt ggagcaatat catcagtgtc cttgagcagt tccagccgct
    15661 gggtaagacg ggattgggtg gtgttttaag cgagattgca gaacgagcca agcagcgggg
    15721 catggtaatt gtgctcagcg atttcttcac agatctttcc gaggtcgaga aggggctcaa
    15781 gcatctccgc taccagcggc atgatgtcat cgtttttcag attgtcgatc gccaggaggt
    15841 gacgttcgat atcgagaaac ctgttgtgtt aagagggctt gaaggtgggg caacgctggc
    15901 ggttgatccg gcatggttac gccgtgcgta tcaaacaagg tttcgtcaat tttgccttca
    15961 gttacagaag atatgtcacg atagcggcgt ggattatgtg tttgtagaaa cggtcaacag
    16021 atttgacaag gttttgcggg aattcgtgtc gtttagggcc cgatgtgggc ggagatagta
    16081 tgttcattac ttagagccta ttccattatc tcccctcgcc cgttcggcgg gagaaggggt
    16141 tgggggtgag ggtggctcga atcgctgcgg gttttctcga tcgactccga cgatggctcg
    16201 ccctcatccc agccctctcc cggagggaga aggggacttt ttgggataga ctcttaatcc
    16261 cggcgcgcga tgagagaact atgcttcagg gcggtctggt aactttgggg ttcgcatttc
    16321 tccaacctgc gatgttgggt tgggctgttg ccgcccttat tcctattctc atccacttgt
    16381 ggcgccgaca ccggtatcac gatctcgagt gggcagcagc cgatttcgtt ttggcggcac
    16441 tgagaacgga gcgacggcaa atcctccttc gacaatacct tctgctgtta ttgagagttt
    16501 tgggaatact cttagtggtc tttgcggctg ctcgcccgac gtttcagaag cccatggtgg
    16561 cacaaactta tcatgaaacc acgggttggg ttttcgtcat tgatgattct ctttccatgc
    16621 aatacacacg agatgacggg accctcttcg aggccgcgct cgaaaggatt cgaaacatcg
    16681 tccagtcaac cccagatggc gatgcttttc tgcttttaag aggggcaaca cctgtgcgat
    16741 gggcggtgcg gcaggccgct tacaacaagg gtgatttcct cgcggaattg aaccgcttgc
    16801 gctgccgcga cacggcggcg gattggcgca cagcacttca gtttgtggaa caacggctga
    16861 gggaattaac ccaggaggaa ccaaggctgg gtcgctttgt agtcgtgctc gtgacggatg
    16921 gtcaagagac aaattggcga aagttgcagg acacaccccg tgcttcggaa acctcaaaag
    16981 ccgaggacga taagggggct gatggtaatg ggcagattac caatctcgcc gagtttttcg
    17041 ttctcctgtt gggggttgag caaccctcca acacggcgat aaccgagttg aatgcatatc
    17101 ccgcgaacgt gattgtgggc caggagactg cattcagcgg tagtgtgaca cattatgggc
    17161 cttccgagac gcgtcccaaa cggcttgagt ggttcatcga tgacaaaaag atcgccgatc
    17221 aggtcttgaa ctggactagc gcaacggagg catttttcag ttggacttat cagtttgaca
    17281 cgccgggcga tcatgtggtt atggtccaac tgtctgcgga tcaactacct gcggacaatc
    17341 agcgtcgtgt cgttgttagc gttaaacagg aacggtcggt tctatgtgtg gatgggcgac
    17401 catcgccgtt gccttttcaa ggggctaccg gttacctccg cgtggctctt ggcgttcaaa
    17461 gggcggcaag cggacgtgtg tctcaacgca tcgatattat cccggagggc cgacttgcgg
    17521 aggtccgcct ggaggagtac gagtcggttt ttctttgtga tgtttcgcga ctgacattag
    17581 gcgaggccca agcactccgc gaattcgtcg aatcaggtgg ggggcttatc atttttctgg
    17641 gaccgcgggt caacattcag gcgtaccatg aaagcctggg gaaatcggcc ttcggcagcg
    17701 tcaatttgtt gcccggacaa ttgcgagagc cagtgtcaat ccaaaaaagc gaaatcgatc
    17761 ccctcgggtt tcgccatccg attgtgcagg tgtttcaggg acgatgggca tcgtcactct
    17821 taatggttcc gattcgcaca tatgtaccat tgcagttgtc tcgcgaagaa gacgtcgagg
    17881 ttgcgcttcg actcactaat ggtgatcctc tcattgtgac cgaatcgatc gggcttggcc
    17941 gtgtggtcgt ggtgacaaca tcggcagatt tgagctggac atccttaccc ctgtggacct
    18001 cgtatgtgcc actgattcag gagattcaaa actatgctac aagtggtcgc acggcttccc
    18061 aaaccgtggt tgcgggcgaa gccattggcc agctcttgcc gaggagttgg caaatcgaca
    18121 ggcataggtt gtctctcgag cccccctggg gagaacgagt ggaggagagc ctacaactaa
    18181 ctggccaata cctgcggtgg tcgtacgcca ataccgagca cagtggcatc tatcgtgccg
    18241 aggctcgctt aaaatcggga aacacgcaaa ctacggtctt cgtagcgaat ccgccggttg
    18301 cggaatccga tattcggcca tggaactttg ttggtttcgt agaacgggtg ctgccccaag
    18361 cgaagtatgt gtcggagcta gagtcgctga gaatcacccc cacgaatgtc agttcggcct
    18421 tgcgggcatc acctctctcc cgatggctcc tttgctttgt ccttgttgtg ctcttgggag
    18481 aattgtattt gggccgtcat acaccgggta cttgagcgca agtctaggag tttgatgtca
    18541 cggtatttcg tgaaatgtcg tgcgtgattg tgtccaatgt ctgattggat taaccgattg
    18601 ggtcatacct taatgccaca tcgtttcacc gacgggacaa gccagtctgt gcgggtcgcc
    18661 ggggattggc catggcctgt ttggataacc atcgttctca tagctctcat ctttttcttg
    18721 atttatcgtt tctacaaaaa aacacgcggt gacttgagct ggaaacagcg tcatcttctc
    18781 tttgctttga ggtgcatcac tagtgccatc gttttcctga tgcttgccca atggatctta
    18841 gtgacggaac aactgggcat gccgatcctg gccattattt tggacgactc gttaagcatg
    18901 cgaataaggg acgtggatcg gcaacctaaa caaggagggg agaaggcaaa cactggggat
    18961 ccttttgacc gggaacgctg gaatcaggcc gttgacgtgc tctgcggtgg gccctcgccg
    19021 ctactcgatg agttggcaca ggactatcgc ctcaaggttt atctactcga tggaaaaact
    19081 gagctgaaag ggccggtttc cgatattgcg agtaccttac gcgtgatgaa gccggagcgt
    19141 gccacgaccc ctctggggaa ggcaattcat gaagtgattg atcaactccg tgggcaaccg
    19201 ccggttggcg tgatcgttct taccgacggg gtggtcaacg aaggcccagg ccttgaggat
    19261 gccgctaaac gggcaagtca ggagctagtt cccctctact ttgtgggctt tggggcggag
    19321 aaaggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc agaagaatcc
    19441 tggtaaagaa agataccata cgtatcctca tggttgagga ggtgccgcgt tttgaatatc
    19501 gattccttag aaatgccttg atacgcgagc caggctttga tttaacgacc gtccttctgg
    19561 gagccgatcc ggaatacgtg gaggaaatga aaggggccat gttggcggtt ccaacctccc
    19621 gcgacgagtt gtggtcctac gatgttatca tcttgggaga cgtggctccc aaccgagttt
    19681 cgccgagtac cctggaggcc gtagcggcct ttgtggagga gaaagaccca ccgggcggac
    19741 taattgtggt cggtggacca cacgccatgc cccacgagta caatcaatca ccacttgaaa
    19801 aggtcctacc ttgcgccgta ggggtatctc cggcgaacat cggacagccg aagacggcag
    19861 cccataggct ggccgtcact cgggagggcc tagttatgcc ttggctggcc tttggaagat
    19921 cacctaccga aaccgaacgg gtgtggcatt cgttaccgcc cgtatattgg cttcaatccg
    19981 tgattgagct caaaccgggg gcacgggttt tggtcgagtc tcgtgaagaa aaccagcaag
    20041 aaggccgcgg cgagccggtc gtggtctttc aatatgtcgg cgccgggaag gtcctgttcc
    20101 atgccacgga tgaaacgtgg cgctggcgcc gaggggcagg cctggctgca tatcgccact
    20161 actggatcga ggctgtccgc ttcatggcgc ggggaaaatt ggaatccgcg agaacgcctc
    20221 ggcttgtcac tgaccgctcc gagtatcagg caggggattc tgtccaccta atgttggaat
    20281 gtcctaagcc cgcggaactg cccctgcgaa atgggagtgt tgccatctgg ttggaatacg
    20341 agggacgtac tcggcgggaa gtcaccttgc aacgtcatcc tttgcggggt gatttgtttg
    20401 aagcaacgct tcagaacctt gccccaggtc aatactacgc ctgggttccc atcacctcag
    20461 ggattgttcc ggcgactggt tttgccgttg tcccgcccct tcaagagtgg gaagcaacac
    20521 aaatggcgcg aaaggaaatg gaaaccgcgg cgcgactttc aggcggcggc ttctatttgc
    20581 ccagcgaaat ccgccgtctt cgagacgatt tgcctcacgg caggggagca ttgatcgaga
    20641 tgctgccgcc acgccccttg tggaactctc ccatggtctt gctgctcctg gttctcacgc
    20701 tttccttaga atggcttctg cggaagcggt atggcttgtt gtagaataac gacaggagga
    20761 gtttcgcttt gtcctgagac aacgatgaca ccgaaatttg cgcggcacgt tgaaacctta
    20821 cgccggcgac ttcgccacag gctagtgctg cgcgcgtgga gctggacgat tcttgttttt
    20881 attgctgcgg tgttctcgct tggggcactg gactatgttt tgcggtttca agaacgaggg
    20941 ctccgctttt tggccaccgg gacattattg gcaaccaccg tgtggtgctt tttccgaatt
    21001 gtcaaaccgg cgatgacgct ctgcatcgac agggttgccc tcgcgcgctg gctggagaag
    21061 ttccaaggtg gtgcacccat ctcgttggcc gcggcatgcg acctcgtgag tcttcccccg
    21121 gacgatctca ggtggggctc gccagcattg cggtccatgg cgatcgagca agcggaagcg
    21181 cactggacgg taccgccctg gcgggaagtg attgaacgag gtccagccct ttggccgagc
    21241 tatcttgctg cggctgccct ggtcgtcgcg ttttctttgc tcctggtgtt tcctgtggtg
    21301 gcgggaacag cactgatccg ccttgtcaat cctttgagtg atctttccta tcctcagacg
    21361 actcatttgg ttgtcgttca ctatccagaa cgattatccc gcggtgatac gtttttcctt
    21421 caagttacgg aggagcgcgg acagttgccg gatgaagtga gaattgccat tcggcctctt
    21481 gatggcgaat ctcacagggc ggtcaaccaa ctcatgaaaa aagttcgaaa tcaattcgta
    21541 tttcgccagg aaaatgttcg caccagtttc cagtttctaa taaccggggg tgacgatcgt
    21601 agccagcctt ggcgccgagt ggaggtggtt gatcctcccc aggtttccga gtggaaggtg
    21661 tggctcaaac cgcctgaata cacaggctgg ccgcctgagg aagctcgcgg gccgcttcgg
    21721 gcattggcag gcactgaagt gcaactgtcc ggtcgcgcaa accggccctt aaagcgtgtt
    21781 gtggtggact gcggacctca acaaagcttt caaggcgatg tcctgccgga tcaatattct
    21841 ttccgcgtgc ctggtggaaa aaaccatcga cccgaacagg agcgatggat tctgcagacg
    21901 agcgggtact accgaatccg gttggaggcc gctgacggaa cgttctcggt caatgagccc
    21961 cgctgggagg tccgcgtgta tgaagatgct ccgcccacgg tggtcctcga gtcgggtcaa
    22021 cgattgctcg tgatgacgcc cgacggcaga ttaccgctgc gcgtcagtgc gcgggatgac
    22081 ctggccattc gacaaatcaa cctggtgagt cgtgacagca gtggaaactc tctcggaccc
    22141 acgattcctc tctaccaggg gcctcccttt ccgccaacgc agattcgtat ggcggagctt
    22201 gagacacatg cggactcggt cgaaaaggaa tacctgtttc gattacctgt atcggcagga
    22261 aacaccgggc ttacgtttcg agttgtggca agtgtcgaag attacaaggg acaagcatca
    22321 gagagtgagc cgcttgagat tcgagtgatt tccccagatg agttcaatca acgactggct
    22381 tctgaagagg agaaacttct ccaacagctt gcccaacttt acgagtcgtt tgtgaagatc
    22441 agccgcgatt gggagaggct ttccgaaaaa ttacgtgccg aaaaacgcct ctcgcgaaca
    22501 gaggccaatc aacttctcac gctcgatacc ttggaacgac aagcctttcg gcggattgcc
    22561 gacccggggt tggggattct ttatgagctg caagatctcg ccggtatggc cgagtatcag
    22621 ggagtgactc aggaccgcgt tgaacgattg aaaaagtttc gcactgattt ggaagcgagt
    22681 tggaagcaac atggggttcc cgcggagtcg caacttggaa tgatcgccaa ttgggctagg
    22741 aacttggtgg agcgcctctt gggagatgaa cagtcgctga gcatagagaa ggaatgggca
    22801 gatgtcgcac aaaatgccga tcaattctcg cttcaccaag cggcctggga aaaggcgttg
    22861 gaggggttgc tccaaaatgt tagcggcatt cggctggtca acaccctgcg aagggaattg
    22921 gaaaaacttc tcaccgaaca attggagctg atccaacaga cgcgcgactt gggccaacag
    22981 acgttaggac gagctcttca agacctcacg cccaacgaac aggatgcgct cacgagaata
    23041 gcgcggcgtc aaacgcttct cgcaaccaag cttgaggagt tggttgctaa agtgctgggg
    23101 gggctccaac agagcgggaa ccaggatgtt acccgtcagg gatttcggcc acatgaaggg
    23161 tcgatgagtg agcgcgagga cgatctgaca attcaatcca gtcaaatggt ttcgcaacaa
    23221 ggggcagtat cccaacaaag ctttgaaaaa ccgaagggag acgccgtccc aaaagagcca
    23281 caattgattg cgctaattat tgatcgtatc aatgggcacg gattgacggc tttgatgcgt
    23341 caagcggggc ttgccattga acagaatcgt ttgagcgagg gcctaaccct tgaggagcag
    23401 tctgccgaga tcctgagaga gttaatcgat ctattagacg gaaagagaga ggacgccggg
    23461 cgacgtcaac aagaccttgc cgaacaagaa aacgagttga ggaatcttct ccgtgcccaa
    23521 caggacctca ttcgcaaatt tgcggaagca cggcaaacgg cagttgaaga ggctttatca
    23581 acgtttattc gtgagcagtc aaagattgag caacgaacgc gggaacttgc ggatcaaatg
    23641 gcgccaccct tgaaaagggt tgccgaaccg ctgttgaaag aagcgatccg gcgtatgcag
    23701 gatgctcaaa cctcggcccg gcaaaaagat gttggaaaga ccgaacagaa cgcgacattg
    23761 gctcagggct taatggagca ggcgcttgag gctgtgggac aagaacggat aacacaagag
    23821 cgaaacaagg cgtatgatcg atggcatgag tttgtacgac gaatcaatgg gataatcagc
    23881 gaacagcgaa accttctgag aaaaactcag tcgctggata tcgagaaacg aaaatcccaa
    23941 acaccggaaa gactagaatc ttggaagaaa acggtgcttg aactgggtcg cgcccaggat
    24001 gccctgggac aacaagttgc ccaattggag agggatatcc gagaagcacc ggtaatcgcc
    24061 gcgattctga cccgggtgga ggaagccact cgaagggctg ccaagtatct ggtaggcctg
    24121 gagtgtggag tttcaacgca agaggcccag cgaatggcct tgagcgacct gcagttgatc
    24181 gcaagggcga tccaaggcga atctagtcag gtaccgccgt catctgccga gcagggaacc
    24241 cagccaaatg ccggtgatga tcagggaccg caatctcccc gtctcgtgtt tcttgcgcca
    24301 cagatcaaac tcttgcgaga cttgcaagca agtatcaacc agcggaccga gagtctcgaa
    24361 aaacgtcgct cgcaacccgc tgagaatccc gccgtgcttg agcaagagtc agagaggttg
    24421 agccatgaac aagccatggt cctgaacttg gtaatgaaac tccgcgacga ccttttggaa
    24481 agcgcgtcgg gcgaggaaag ccgggttaca gggccggcac gggagggagc ggagacgccg
    24541 ccggctaccg agcgtcaacc gaatttgcca aaaccggggc aacccggctc ggtgagagat
    24601 ctttttaacc cttgatgagg acgacattgt acagtcagtt tcttccgtta ttttttcaac
    24661 caaaccgtgc gaagatgagc ggtgtcgtga acttgcttcg ttttcaaaga gccctggcaa
    24721 tgatttgttt aagcgcgggc tttgtggggt actgttttct ttacagtgta tgcctcgggc
    24781 aaacccgcga gaccgggccg ttttcttatc aacaggacgc gcgttcgccg tcagcttcgg
    24841 cacgtgccag cacttcacaa gttgaaagag agcttggttt ggccgcaaca tctgaggata
    24901 gtgatccctt ggtcctggtt ctggagttga tgaaagaatc ccaggttcgc cttgcccgga
    24961 aagatacagg tgaacagact cgacaaattc agagcaagat tgtcggtgta tttgatgccc
    25021 tgctcaacca agcgatgcaa agcgcgcaac cacaaccatc ggcaggtaca actcaaaatg
    25081 agcgacaaga accggaagag aagactcaaa cgacccaggc gcagccacgt caagaaacag
    25141 aggccggttc gcaacgtcct acacagcaaa gtggcatttc ccaacgcgga gagttgaacg
    25201 aggtacttca gaaagtttgg ggtgagctcc cggagcgcga acgcaatgcg gtcctccaac
    25261 atgcagccga ggcgattgtg cccaagtatc gtgcnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnctgccg catgtattga gcggggtctg cagtatttgg tttcccggca
    25441 gcacgtggat ggttcattcg gttcgggcgc ttaccgaggc aacatcgccg tgacggcgct
    25501 ggccgggatg gctctcatgt gcgaagggag tttgcccaat cggggtagat ttggcgaaga
    25561 ggtcaatcgt gtcctggatt acgttttatc gaattgccat gaaaatggtc taatcctgta
    25621 cgttccttcg gcaagccggg gacccatgta cgaacatggt ttcgccacgc tatttctggc
    25681 ggagtgttat ggttcgagtc cgcgagatga tttgagagaa aagctcagcc gagccgtgcg
    25741 actcatcgtc actacccaaa ataacgaagg cggatggcgc tacttacccc agccattgga
    25801 cgccgatatt tctgtgacga tttgccagat tatggccttg cgggcagccc gcaatgcggg
    25861 aatccacgtt cccaaagaaa cgattgaccg atgcgtcgat tatgtcaaaa aatgccagaa
    25921 tcccgatggc ggcttccgat acatgctgat tcccggagag agcgcctttg cccggtcagc
    25981 ggctggcgtg gtggcgcttt atagcgccgg gatttacgag ggtccggaaa tcgaacgggg
    26041 cattgagtat ttgtctcgtt tcatcccgga gccaggcgtg attaggcagg aaccatacta
    26101 tttctatggt cattactact gcgcgcaggc gatgtggcag cggggtggcc aactgtggca
    26161 acgatactat gccggcatcc gcgatgagct tatcgcaaaa cagcagccgg atggctcgtg
    26221 gagtgatccc atctcaccgg agtttggaac agcgatggct ctgcttgtgt tgcaaatccc
    26281 taatcattac cttcccattt ttgaacgatg aagcgtctgg acgtcaaacc aatttggtgc
    26341 cctgaagagg aacgaaaaga cagggatatt gcaggccctt gtcgtggtgg ccggcgccct
    26401 cggcatatat ggctttttag ggttgtggtt accctttggg catgtggatg tttcggcgtg
    26461 gcgggtttga gtaagtgtgc ttctcaagag ctgctcctta caaccgggac gatcgacgag
    26521 gccaaattga tggccgtcga gggagacagc atcgtttttc aaaccaagga cggtcagcgg
    26581 cggatacctc gtgatgagat tgtttgttgg ggagtgctgt gtgagccgag aggcggtgaa
    26641 tggttgtgtt tgcacaatgg aagtctgatc gccgggaagt ggcttggtct tgcgaatggc
    26701 cggcttacgt ggcgaaatga ttaccttggc gagttttcag ttactgttga tcaagtcgcc
    26761 ggaatgatct ttcgcttgcc gtcgtctttt atcgagaagg acctcctgat cgcgcagata
    26821 ttggatagga caaagggtgg ttatcttctc ctggccaatg gcgatcaatt ggacggcgca
    26881 ctcctcagac gtcaggacaa cggggacctt ctggtgacca cccgctttgg agagcgtacg
    26941 ttttcggtgg aacagcttcg cggcgtttca ttgcggagag tatccgcggc aacgcaaggc
    27001 cccatagatt gcgcggtggg acttaaagac gggtcacgaa tcatcatatg caaatttacg
    27061 cgcgggacgg aggacccggc ctctaaggat ttggctcctg ccttgggtga tcgtgagatt
    27121 cagggcgtac tttcaaacct tctccagaac cgtatcgaag atatctgttt ttatcagccc
    27181 tttggcaacg tggtttatct gtcggatttg gccccggaga cttttatcgc agtgccattt
    27241 ttggaatatg tccggccgct aaagatggat cgcaatgccc tcgggggatg gttacgaaca
    27301 gaggaaggcg cctttctgaa gggaatcggg acctatagtg cctgccgggt ccggtataac
    27361 cttcagggcc ggtttagccg atttgcggca gaggtggcca ttgatgcatg cacccagggt
    27421 aagggcagcg tcatgtgcca tgtcttggtg gatggagagc gaaaagtgag cgtgggtccc
    27481 atccgggggg gtgaccgccc tgtgcccgtc gtcgttgacg tcaccggggc ggcaagtttg
    27541 gaccttgtgg tggattttgg tgaacgagcc gatgaacagg attatgtgaa ttggctcaac
    27601 gcgcgactta tcccggcaac tacgtcttta acccaagaga aaggcggcga ctgagttttg
    27661 cggtgcccga cagttggggg cttgctacgc tgccatgtca cgactccagc caacgtttgg
    27721 cccactacgg tgccaatttg gcagagacct cagataatag ccgacgcctt tgacaaaaac
    27781 gcaaggtttc taccagtccc gccacatttc ggtttcgaag cgttagtttc cttagataat
    27841 acacttacgt cgaagtcgca ggattatgct attttggcgc gcaaattgcg tcggagctga
    27901 gccgagtcat tattgactgg gcatgcggca atcatccaag gcgaatgtca aagaaaactg
    27961 ccgttttttg tgatagcttg acgttaccaa cattggaggt tttgcacaca tggcgaagaa
    28021 gaaaggcgac aaagaggaat ccaagcttca actgcagccg ctagcagatc gggtcgttgt
    28081 ggagagggag gagtcagaag agaagacacc cggtggcatc gtcctgcctg ataccgcgaa
    28141 aaagaaaccg tcacgcggtg ttgttgtctg cgtcggtccg ggtcgcatgt tagaagacgg
    28201 tacgcgggcg ccgatgcagg tcaaagttgg tgaccgcgtc ctcttcagtg cttatgcggg
    28261 tgatgacttt aagattggcg atcaagaact ggtcctgctg cgggaagaag acatcttggc
    28321 ggtgatcgag tgattgaccg tttccgccgg ttgaaattgc taggcagaca aagaggagta
    28381 gttgacctat ggctaaactc attgcttacg atcaggaagc gcgcgatgcc ctccgtcggg
    28441 gcgttgccaa actggcccaa gcggtaaagg tcacactggg gccgcgaggc cgaaacgtca
    28501 tcatccaaaa gagctttgga tctccgacgg tgaccaaaga cggtgtgacg gttgccaagg
    28561 agatcgacct ggaagacgtt tacgaaaaca tgggtgcccg catggtccgc gaagtcgcct
    28621 ccaagacgag tgacattgcg ggcgatggca ccaccacggc aaccgtccta gcggaggcta
    28681 ttttcagcga aggtcttaag gccgtggtct cggggatcaa cccgattggc cttaagcagg
    28741 gtattgaaaa ggccgtcgag gacatcgtcg ccaagctcaa ggccatgtca atcccgatta
    28801 aaacgagcaa agaaatggcc caggtggccg caattgctgc caataacgac atggccatcg
    28861 gggagctcct cgcccaagcc atggacaaag tgggcaagga cggggtcatc acggttgaag
    28921 aaagcaagac gatggagacc aaggtcgaat ttgtcgaagg tatgcagttc gaccggggtt
    28981 atctgtcgcc ttatttcgtg actgatcccc agaaaatgga atgcgttctc gaggattgct
    29041 acatcctcat tcacgagaag aagatctcta acgcccgcga gcttgtgcca ctcctcgagg
    29101 ccgttgttaa cgcagggaag ccacttttgg tcatcgccga agacgttgat ggtgatgcct
    29161 tggcgacgct ggtcattaac cggctgcggg gtacactccg ctgctgtgcg gtgaaggctc
    29221 cgggctacgg tgatcggcgg aaggccatgc tagaggacat cgccatcttg accggtggcg
    29281 tggccttgtt cgagagctta ggccgcaagc ttgagcatgt caccctggcc gaattgggtc
    29341 gcgctaagaa ggtcgtcgtc gataaggaca acacgacgat catcgaaggc gcaggcaaaa
    29401 ccgaggagat taaaggccgg attgagcaaa tccgtcgtga aatcgagaaa acgacgagtg
    29461 attacgaccg ggagaaattg gaagagcggc ttgccaagct ggcaggcggc gtggctaagc
    29521 tgaatgttgg tgcggccacg gaagccgaaa tgaaggagaa gaaggcccgc gttgaggacg
    29581 ccttgcacgc cacccgagcg gccgtcgagg aaggcatcct cccaggcggt ggagtcgcgc
    29641 tcctcagggc tgctatgcag tgcaaacccg atgggttgac caccgatgaa aaggtgggtt
    29701 atgaaatcgt cctgcgggca tgccgctcac cgatcatgca gattgcgtcc aacgctggcc
    29761 aggacggtag catcgtctgc gagaaagtca gtgaaatgaa gggcaacatg ggcttcaacg
    29821 ctttgacggg tcagtacgaa gacctcgtca aggccggtgt catcgacccg accaaggtgg
    29881 ttcgggtggc cctgcaaaac gccgccagtg tggcaactct aatgttgacc agtgatgccc
    29941 tggtggccga aaagcccaag gaagaaaaat ccggtggtgg gcacacaccg cctgagtatt
    30001 aatccactgc cccttaacgc gtgctgctca actcggatgg gcggcacgca atcgctgaag
    30061 tcgccccctt cccctccctt cgagagcgtc cccaaccccg ctggggacgc tcactttttc
    30121 ttcgggagct tcaaggcacg ttccttctta cctaaaatag cggcatgttg tcgcaacggc
    30181 tttttcacca cagtggcttt atttccggag atcacttcat gaggacgagc atccaaggag
    30241 atcgatgggc gaggaagact cgagtacgac tcttgaccgt tttctcgatc atcacctgcc
    30301 tcggctgcgg agtggccgct cttggcgcgg aggattcgcc tttgccaaag tcttggatgg
    30361 aggcatggat gcaaccacca ctggaatgcc gtccgctaca aattgttcat ggcttaacac
    30421 cccagcgcgt ggtggccgac agcgtcgcac agattctgca acagcccagg gcgcagccga
    30481 tatcggatga agggatggcc tattaccttc ggcgaggccg cggaggaatt gtgtgcaacg
    30541 tggcctttca ggattacctt cggtcggagg ccaactggca gacgttgatc gaaggcgttg
    30601 aactttgtgc ccgtttgaag atgcctgttt ggatctacga cgaaaagggt tacccaagtg
    30661 gtgcggcggg aggactggtt ctcgagggca atcccgagtt tgaagccctt gtcttggcat
    30721 gggaaccgcg gcagacgccc gcgtttttcc tccgccgggc attcgaacat acccatgccg
    30781 cgaataacta tcatgcggtt cgccgctata tcaacctcat ggatgatcgt gccaccgcta
    30841 agtttattca ggtgacgcac gaagcctatt acaagcgatt ggcccgccat ttcggttcaa
    30901 ccattgtggc cttttttacg gatgaacctt ccctcatggc cgttgacctg ggggcaattc
    30961 cggagcctgc gcgcagtcga gttcccatag aggatgcacc cgaccctaac gtaaagccct
    31021 taccgaccgt gccgtggtgt tatgatctag aaaacgttta tcggagcaag tggggtgaag
    31081 accttcgggc ccaacaaaag agcctttttg tcggtaatga tccagaagat cgtcgcatcc
    31141 gccgccagtt ttggtcgatt gttgcagagt tagttgcgga ccgctacttt ggcgctcttc
    31201 gccggtggtg tcaaggccat catgtcgcct cctcaggcca taatctgtac gaggaatcga
    31261 tcctccatca tgtccccctt tatgggaatg gtctgaaggc catcgcgcaa atggatgtcc
    31321 ccgggctgga catgttaagc tcgaatcctg aggtcgtcat tcacagtgga tggctgacgg
    31381 cggccatgcc ggtatctggg gccctgttga caggatgccg tcgcgtgatg acggaagtca
    31441 gcgatttttc tcagaagatg tcaggggcgg gaccggtagg gcttcccgaa atgatggcaa
    31501 cagcggcatg gcaagcggcg tggggtgtca cggagtttac gctttactat aacccggagg
    31561 accgctccgt ggaggagaat tggcagtacg gccaattcgt ggggcgcctt aatgcgatcc
    31621 tccgcgaggc catgccccgg cgcagggttg ctctttacta ccccatttat gacctgtggg
    31681 ccgagtatct tcccgtggca ggtcctctcc accagtcggt tcgcagtgag aaagtccagc
    31741 aactgattgg ctcttttatg cgcctcggac agaccatgca gcggcggcag attccgttcg
    31801 ttttgattga ccatgaatac ctacagcgat cacgggtaag ttccgacggc cgcctctgtg
    31861 tgggcaatct ggaactggaa agcgttgtca tgccggccgg ggtggagctg ccccatgagg
    31921 cgcaagctgt gattgagcaa ttccaacaag caggtggccg cgtcatccga gatgctacgg
    31981 atgccccgct aacggcagct aagttagtcg aggccatccg gcccttatat cacatagaac
    32041 cggcaaatga tcggatcgtc tttggagagt ttgagaggga agggcgaacg attttgatcc
    32101 ttgccaatgt ttcgcgggaa ccctacaaag gtgttctacg agcaaaacaa cacaagacct
    32161 ggacggcgtt ggatccgatt acaggtgaaa tacgggcagt caccgcgaca gagcagggga
    32221 tccccgttca ccttgatggc tacaaggcca tcctgataag tcaataaggc aatttcttgg
    32281 catatgcttc ccgtcacgcc cgtcaaaagg gaaaaccgag gtggcgagcc aacctcggtt
    32341 tgtctttcgt tgatctatgt tgtgacaacg ccccgaaccg gccgtcaaat ttattgagtt
    32401 tattaccctt tcgcggtgac ctcaatcttt cgacgacgga ggctagccgc cttggggagg
    32461 tgtagggtca agacgccatt ctcatattca gccgatatct tggaggcgtc gattgcctcg
    32521 ctgacctgga aggtccgcaa gtaatcccca acaccgtact cctgttgcaa gaactcgact
    32581 ccttcagggt agcgtgggtt aaccttggca tggacggtca acatgccgtc ctcgaattga
    32641 atgtcgatgt cttctgattt ggttcccggc acgtccgcaa tcaacaggat ttcatctcct
    32701 ttttccacga tgtcaacagg cgggctgtaa taacggtcac cacgcaaatg ttcgggctgg
    32761 ggctgggcca tacgttttcc accatgttgg tgtctcatcg ctatctctcc ttctcactaa
    32821 gcgatcgaca cgacatccac ttgcgttatc gcacattgac ctggattttg cgcggccgaa
    32881 cggtttccgc ctttggcaag gtgatggtta acacgccatt ttggaggtcg gcctggatgc
    32941 gatcgacatc gaactcccca ggaagtctga tgacccgact gcattcgccc gtctgtcgct
    33001 ctcgccgatg gtaggtcact ccctcattgg caggctgggg acgattgaca cgaagggtca
    33061 actcactacc gaccacagag atgtcaactt gatcggtggt gacccctggg acttctgctt
    33121 caacgacgaa ggcttccggg gtttcccata cgttgaccgg gattcggttc cggctcgtca
    33181 agccaagacc cgcttccgga agttcactca gaaagttatt aaacagtcga tcaacttcac
    33241 gccggagttg atccagggac ggcggaccga agggaggaac tgccatgaca cgtctccttc
    33301 ttcccaaaga cccagatacg aaccgaaaag ccttgtccca accaccacgg tcgatgtgtt
    33361 ctttcatgtc gggactgcaa aacctatgtt agcacgctag caaatactat gcccgttcgg
    33421 tgatgtgcgc gtaacatact taatgacaat aacttgcgcg cattggatgc gcaaaacatc
    33481 tgccacaatg gcagcgtcga gtattcgttt ccaagtgaag ttaccggttt gcgtgatggc
    33541 gacccaacct tatgccaacc gccaccgggc ttggcgtaaa acggcaaacg gagaaccgga
    33601 catcaactcg taccaaatcg ttacaaagtc acccccggcc aattgcaccg tgctggggta
    33661 ccccagatct ccgcccgcac catccccgta aatgacgatg ggctcagacc atgtcctacc
    33721 ctcatcctcg ctaatccggg cttggcaacc gaaaggtgga cgtcggtgtc cataactcat
    33781 aagaagacga ccatccgcca tccgcaacaa atgggaaggc aatccccaga ctccgatagg
    33841 atgggggacg ctccatgtct ttccgccgtc ctccgattcc gattgcaggg tttcgcc
//
LOCUS       DAOSUT010000022        32784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_931_length_32784_cov_9.322497, whole genome shotgun sequence.
ACCESSION   DAOSUT010000022 DAOSUT010000000
VERSION     DAOSUT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 32784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32784
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_32784_cov_9.322497"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLD05_03360"
     CDS             <1..360
                     /locus_tag="PLD05_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOP76499.1"
                     /translation="LVLPGCTGRKTKPKGSVEGKVTFNGHPYTEASVVFFNQETGQGS
                     SANIQPDGSFRLPERIEVGTYIVYLAPKMEEDPAAEPKPVSIDKTVPEKYWSESTSDI
                     RVEVKTGSNQFNIDLKP"
     gene            1016..>1798
                     /locus_tag="PLD05_03365"
     CDS             1016..>1798
                     /locus_tag="PLD05_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76500.1"
                     /translation="MHDLMELVTFIVDHTPALWQAADFIIPENALQGYWVSARSRFER
                     WASTLKELTSRSLTASREYRFRNQLAGILEEILTNEILTRVWTAALSLYDQQRKVDWA
                     GPIGQSAFLAQLELRVRSLGILSDGHFLPGEDIQKLLQLCERNDRWCDLLLSRLSEFG
                     DVSAYAVDPQRWQEFSADFKAIPTEKQRNQAWQLMRTSLLAAYAVPMFRYHMQLSQTV
                     LVAGYILDCFPQDVLEICQDLNFYRESRFVARCRRAQELLAEV"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..3721)
                     /locus_tag="PLD05_03370"
     CDS             complement(<1899..3721)
                     /locus_tag="PLD05_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOP76501.1"
                     /translation="MMGQRLRRALNGFSRHMVVWGRSVRLLGYSVLVGVGAGAAAVLF
                     TLALELVEHSALGRIMDYVPPPEHTADPIARFQLPRQWWAVLLVPTLGGLLCGVIVYF
                     LEPASEGHGTDAMIRAFHRLKGVIGPRVPLIKMITSIVTIGTGGSAGREGPMTQIGAG
                     IGSLLAQYLKLGDWDRRILMLAGAAGGLGAVFRTPLGGAMFSVEVLYSGTAIEFSAVA
                     PAFVASVVAYTIFTSVLGQTVLFVLPTAPDPQTGALPCAFHGAHELPFYLALALLCAA
                     VSYIFVNMFYFGRDRIFRLARLPQWLRPALGGLGLGIIVLVGYPHVMAGGYGWIQKAI
                     LASPDISLRLAISLVVLKMVATTLTVGSGGSGGLFAPALFIGTMTGAAFGMACQALFP
                     ASAPPVSATALVGMGGVLAGTFKVPLAALIMVSELTGSHDLLVPMMLVGAVEMVSLPG
                     NVSLCREQVTAFIDSPAHLGDFVVDVFKEMHVRDVCPFSTQPIVLREDTPLPQILRTV
                     ADAHQTTFPVVDRDNKLVGVFSLSDLRAALVGDGAGNLVRAIDLAVPVPIVTPDDDLH
                     TVLQIMAEKHLEELPVVMSHAGQQTISLIRRDFILAAYDRRV"
     gene            complement(3899..4486)
                     /locus_tag="PLD05_03375"
     CDS             complement(3899..4486)
                     /locus_tag="PLD05_03375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOP76502.1"
                     /translation="MVKRAPIARICVFFFITVMGVAADLTTKAWIFNRLGMPGEKPVW
                     WVIPDVFGFQTSLNEGALFGMGQGMTRWFAALSVVFAVGIVVWFFRGGAGHSWWMTTS
                     LAAITAGIFGNLYDRLGLHGLRWHEANGLHQVGDPVYAVRDWILVMIGRWPWPNFNIA
                     DSLLVCGVTALVVYTLFFSELACPSEEKVQVEEIK"
     gene            complement(4708..6228)
                     /locus_tag="PLD05_03380"
     CDS             complement(4708..6228)
                     /locus_tag="PLD05_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76503.1"
                     /translation="MKRHNERAANHEALRHSRRGFLAITAQLAAFGFVGCGRVTACAE
                     DSVSLFDGRSMEGWRASENKDSWRVEGGCLVADGPRSHLFYEGPVCDHNFRNFDLTLE
                     VLTKPGANSGVFIHTQYQETGSPSAGYEVQINNSYPMVGDNPEFRRTGGLFAVQDVFK
                     AFLPDDQWFQMRILVIGKRIRVWVNEFLMVDYVEPESPPRMKNRVGRRLSRGTIALQA
                     HDPNSRIHFRNIRIRPLPDQADPFADPRPDVSPYGITEGLIDRLSMSAIPATDFHIHL
                     RGGMTVEKAIERQAVTGINAGVLENHGRGWPLDNNEKLAAFLESVAGRPVFVGIQVND
                     RDWAEVIDPKLLDQLDYVLADTMIMPMPDDNSPPVKLWQTDQYTIDDPEAWMERYMKH
                     NLRVLSEPITILANPTWLPKPIEHLYDKLWTEERMRLLIETAVKRGIALEINAGKYPP
                     EKFIRLARELGAKFTLGTNNFDDRPISLARCFDMIDRVQLQGRNFLLLRPKDKQVG"
     gene            complement(6316..9054)
                     /locus_tag="PLD05_03385"
     CDS             complement(6316..9054)
                     /locus_tag="PLD05_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76504.1"
                     /translation="MKYFLTILITLVTCWISSPTWGETIWQEGESPVSHKMNRHPWWY
                     DQVKKEHLSGGDWISNFSEEKEGMVEYSVSLKDGGDYVLWLRINPVRSRVSLSVDGSP
                     WRELDLSAATQVVNIAADNKIDLRFIGWVNAGRLTLSRGDHTLAFRFHSELQNHGGLD
                     AFVLTNEPFSPSGLARPGTAETRADVVTEIGTWPFEPSVDPIRAGALLDLRSLNEQTA
                     GEKGFIRLSEDGMSFLRGDGQPIRFWAVVDYGWRMEPDVMRRHLRWLAKLGVNMVRVH
                     AQLCVEKEGAKLTDVDEKVIDQIQRYVAECKNHGIYVTISPYWSHTSKIPASWGLDSY
                     TGQSGPEGLIFFEEKLQDAYKTWVKELYTRPNPYTGIPLSQDPTVAIIQVQNEDSILF
                     WTSQNIREPFAGKLRRLFGRFLVEKYGSLEKAQDAWGDARHKQDDFASGEVGMEIIWR
                     MTSQAPKPNAAWAKRLADQLEFMSRLQHQFYADMAKFYRETLGCKQLLNATNWRTADP
                     VLLEDCERWTYTAMDVAALNRYTGVIHVGPMNGWRIDPNHYYISQSCLLNPEEFPGAV
                     KQTVGQPMILTEVAWVHPALYQTEGPFLAAVYMSLTGVDAFYWFSYGDEFWTADPVWP
                     WWKVGGLNSLKKWEGSVPTQAGMFPAAALAYRLSYIRAAEQPAVYEERSLQGMWERRV
                     PIIAEAGKFDPNRDPGDFAPQSPIRQEVDRLAFFVGPVHVKFEGDEKNSRMLDLSKFI
                     DRKKGIVRSMTGEISLDFRNGVCTVNTPKCQGCTGFLKQGGSRFSFDDMTIVSENDYA
                     TIMVVTMDNRPLRDSEKVLVQVGTTARMTGWTERDAEFESGGQKLRGKQIVDTGRPPW
                     RLAENRIKMTVKNSGLGRAVVLDVDGYPRQDLPLQRQGDGLTLEMPRDCLYVILMK"
     gene            9421..10386
                     /locus_tag="PLD05_03390"
     CDS             9421..10386
                     /locus_tag="PLD05_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOP76505.1"
                     /translation="MGEARIVSFINYKGGVGKTTTTYHIGCSLAEHHGHRVLLVDIDP
                     QSNLSLLCMSYDRYNQFRTQQGTIRDLYRRFRQESRLGTRDFIVREPIQLNNGPLRNL
                     DLLPCDLDMLGEDIGGILPTVSAARTGNAFQVLEQVAERVLREWDFLRLALDEVRSEY
                     DDILIDCPPNLYLMTQNALAASDWYVITVVPEHLSSVGVPILIRKIRDIRERIRRVAA
                     ILGREVSFARFAGIIAVKMRRAGQHQDVLNRLREDLSSYIGEGSLADPVFATATTELI
                     GYSEAAEQNLPVWHARGNNPAMAAERREYEAITEEYLRRCHSGEL"
     gene            10471..10989
                     /locus_tag="PLD05_03395"
     CDS             10471..10989
                     /locus_tag="PLD05_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76506.1"
                     /translation="MAKRQSASTLEVLEELSGVFNLLPTEEFRNEVTTSVQQIIGFLQ
                     DLRVAIEKIPTREQMIDRRIPESLDRVRDFLKPFESAGRARSSRQAQRSVKKVVDLEE
                     FKDHLMTLPIGQIEGALRDSGLTLEQLKEVLTRLGGSSRGARSKEQVIQRIVDEIRTR
                     RELSGLRDGATP"
     gene            complement(11108..12880)
                     /locus_tag="PLD05_03400"
     CDS             complement(11108..12880)
                     /locus_tag="PLD05_03400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOP76507.1"
                     /translation="MAVLVQKFGGTSVADAEKILAAARKAIRAKLQGYQVVTVVSAMG
                     HTSDHLIELAKQIDPEPPAREMDMLLSTGEQVSVALMAMAIHRLGHRAISMTGAQMGI
                     YTDSTHTKARIRSISTDRIRQLLDEGNIVIAAGFQGITDTADITTLGRGGSDTTAVAL
                     AAALRAETCEIYTDVDGVYTTDPRLVPEARRMSRISYDEMLELATAGAGVMHNRSIEF
                     AKRFSVPVHVRSSFSDLPGTIISWTPESRDVPVCGAAAILKEARITVRGVPDKPGNAA
                     VIFSRLAARNIPMDMIVQNVAAGGRADISFTVFQEELPAALRVVEETAREIGAEGVTY
                     DENVAKVSVVGLGMAKQPGVAQRMFRALADKGINILMITTSEIKISVLVQRDQAVEAL
                     RTVHQAFELHKEPPSVAEESRPEVVANDRERIIGRLHGMEDLVIESICLDETQGCVTL
                     SEIPDRPGLAAKLFRQVADRNIVVDMIVQSVGREDKAMMSFTVPQGSYEEAMTACQAF
                     AEEIGAAPPTGRPSVAKLSILGTGLKSHISVAGRTFQALAAAQINVEMISTSEVRLNV
                     VVSGDTGRQALAVLRREFAPELIE"
     gene            complement(13002..14219)
                     /locus_tag="PLD05_03405"
     CDS             complement(13002..14219)
                     /locus_tag="PLD05_03405"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOP76508.1"
                     /translation="MRKYAIVIPDGCADEPQESLGGKTPLEAADIPAMDAVARAGVVG
                     QANHVPRSLPPGSDVANLSLLGYDPVAHYTGRAPLEAAAQGIPLGPNDWAIRCNLVTI
                     IDQVMRDFTADHISTGEARELMTTLQEKLGTPQLQFFPGVSYRNLLIYRGGDKPAPFS
                     RDTVTVPPHDLTDQSVIDAYPRGPGSDLLTRLMCESAEIFANHPVNVARQKAGKLPAT
                     NIWLWGLGGAPQLPPFQQRYGVTGAMITAVDLLRGLAALFGWRRIDVPGATGYIDTDY
                     AAKGRYAIEALKEVDLVCVHIEATDEASHEGDAEAKIQALEAIDQKIVAPLWEALQSY
                     GDYRILVSPDHPTPLRLKTHAHGYVPFAIAGTGIPTSGATGYNERTAEESGIIFEEGW
                     RVMEYFLRGESPP"
     gene            complement(14296..15594)
                     /locus_tag="PLD05_03410"
     CDS             complement(14296..15594)
                     /locus_tag="PLD05_03410"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOP76509.1"
                     /translation="MDSLKVAIIGFGTIGSGVAKLLLEHGDRIARRAGRPIELVRIVD
                     KDLVRPRNVTVPKHLLTDDLDAVIKDPNIAVAVELIGGVEPTRSIVLSLLEHGKDVVT
                     ANKALLAEHGPELFEAARRLGRSISFEAAVAGGIPIIAAIGESLTANEITSIHAILNG
                     TSNFILTQMEERGQEYAAALAEAQRLGYAEANPSMDVSGADAAQKLAILAQLAFGAQI
                     NWRDIPRVGIDTVQAEDMRYARELGYHIRLLAVAELIDGELELHVSPTLVREGTPLGE
                     VRGAYNAVRVVGDAVGRVFFHGLGAGQMPTASAVVADIIDTAVGRAAITFRTLRLWSN
                     DGPHIGLRNPDMARSRFYLRFHVQDRPGVLAEIAGILGRNQISIASVIQHQTEEETEG
                     VVPLVIMTHTAAEGPMRKAMAEIDRLPVVRPGSVWMRVRD"
     gene            15876..17612
                     /locus_tag="PLD05_03415"
     CDS             15876..17612
                     /locus_tag="PLD05_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP76510.1"
                     /translation="MKTLVTMMCRDVYLTWVSWLLMLTLLASLGLVEASQGSGAERPN
                     VVLVITDDQGYGDLACHGNPVIKTPNLDRLYQESVRLTDFHVDPTCSPTRAALMTGRY
                     SCRTGVWHTIMGRSILRRDETTMADVFRRAGYRTAIFGKWHLGDNYPYHPWNRGFEEC
                     LIHFGGGIGQGPDYWGNTYFSPTLNHNGEWKKEEGYCTDIFFKRAMEFIQAHREEPFF
                     VYLPTNAPHAPLQVEERYAEPYRQAGLPDALAKFYGMITNIDENMGRLMGLLRELKLE
                     SQTILIFMTDNGTAGSGFNAGMRGKKGSQYDGGHRVPCFIRWPGRLPAGQDFTHLAAH
                     IDLFPTLLDLCDIPAPSEVKFDGISLKPYLAGGTATFPERTLFVQVNRIDHPVPWRLS
                     AVLTERYRLIDGQQLYDIKEDPGQTKDIAAANAEVVNELRARYEAWYADVSQRFGEYC
                     ELVLGSDEENPATLCSHDWHGESVPWDQSQMEKGMVGNGFWAVEFAKAGRYRFALRDR
                     PAHASTKLPQGKARLVVEGLFEEEKPIPANAEEIIFEANLPAGKARLQTFLTLPNGTQ
                     RGFYFVVVTRLP"
     gene            complement(17660..18997)
                     /gene="thiC"
                     /locus_tag="PLD05_03420"
     CDS             complement(17660..18997)
                     /gene="thiC"
                     /locus_tag="PLD05_03420"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOP76511.1"
                     /translation="MSTQIEAARAGIITPEMEYVAKSEHLPVETVRDEVARGRMVIPA
                     NKIHLKKDLRPIGIGIACRTKINANLGNSALQSDVACELEKVFCAISLGADTVMDLST
                     GPDITAIRQRIIDASPVPVGTVPIYQVAEELGDIAEMTPQHFLDVVEQQATQGVDYMT
                     IHCALLREHLPLVSRRVTGIVSRGGALIARWMTVHQAENPLYTHFDDLCDIMRQYDVT
                     WSLGDGLRPGCLADASDEAQFAELKVMGELGRRARERGCQVMIEGPGHIPMDQIERNI
                     RLQMEWCDEAPFYVLGPVVTDVAPGYDHISSAIGAAMAAWYGAAMLCYVTPREHLGLP
                     TLEDVRQGVVAYKIAAHAADVARHRPGARDWDDALSRARYRFDWNEQFRLAIDPERAR
                     QMWEETARPGTSPNAKYCSMCGPKFCSMRLSQDVAQSQQSANDPQPSHVPGDA"
     gene            complement(19235..20797)
                     /locus_tag="PLD05_03425"
     CDS             complement(19235..20797)
                     /locus_tag="PLD05_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOP76512.1"
                     /translation="MDFQTTLVSIWREACRHIEITESAAAIAQVLSAQMRFQTLVVRR
                     LDLSQMQLQTVAVAPKSPPGLGPRTTPLSRVSMSRLLDWFSRRVIWNIPQDGAFDGLD
                     AHELFGEIHAPMLIGGLLAGQTPAGILAVVGEPNRAAFSEEDVRLVQQLLEPFAVALE
                     NDQRLREMATLREAAEAEKRSLLAKLGRQSLSDAIIGAETGLKHVMERVELVAMSDAP
                     VLLLGETGTGKELIARTIHQRSPRAKGPFLRVNCGAIPRELIDSQLFGHERGSFTGAV
                     ETHQGWFERANHGTLFLDEIGELPLDAQVRLLQVLQDGTFERVGGCHPITVDVRVVAA
                     THRDLQEMVRQGQFREDLWYRLAVFPIYLPPLRERREDIPALARHFAQRAAVRFGLPL
                     QLPTEEDIRALLSYHWPGNIRELAAVIDRAAILGNGRGLEVLKALGVTAVPSVSKTET
                     LQPQEGDFPTLDEVMRRHIEEALRRCAGRIEGPRGAAALLGINPHTLRSRMRKMGICW
                     AKFRTENGVSRS"
     assembly_gap    20915..21014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21015..21929
                     /locus_tag="PLD05_03430"
     CDS             <21015..21929
                     /locus_tag="PLD05_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOP76513.1"
                     /translation="AKDWFEKTGETVEIRQSHGGSGKQARAVMEGLEADVVTLALAYD
                     IDSIATRTQLIDQNWQDRLPHRSCPYVSTIVFLVRKGNPKNIRDWNDLTRPDVEVVTP
                     NPKTSGGARWNYLAAWGYALRRSLGDLKKAKDLNSPAVAQAQKEAKEFVRQIFARVKV
                     MDTGARAATNTFAQRGIGDVLIAWENEALLTVKELGADKFEIVVPSVSILAEPPVAVV
                     DQVVDRRGTRQVAEAYLQFLFGIEAQRIIARHYYRPTLPEVAQEFAGQFPQLELFTID
                     DVFGGWQTAQKEHFDDGGLFDQIMAATR"
     gene            21939..22799
                     /gene="cysT"
                     /locus_tag="PLD05_03435"
     CDS             21939..22799
                     /gene="cysT"
                     /locus_tag="PLD05_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOP76514.1"
                     /translation="MTGRIDQYGFRGLFRDDVLPGFWLTFGITIAYLGVMVLLPFAAL
                     VAFVTGSADGWQVLQESRVWAAVRLSVGGALVAALIDAVLGVLTAWVLVRYRFPGRQL
                     LDALVDLPFALPTAVSGIALTTLFAPKGWLGAWLQPLGIQVAYTQLGILVALVFIGLP
                     FVVRAVQPAIEDLDPEWEEAAASLGASRWATFWRVIFPSLWPSVLTGFAMAFARGVGE
                     YGSVIFIAGNLPHRTEIAPLLIVIKLEQYDYHGAAVIATIMLLVSLGVLLAVGAFQNW
                     SQPWQKAATH"
     gene            22811..23749
                     /gene="cysW"
                     /locus_tag="PLD05_03440"
     CDS             22811..23749
                     /gene="cysW"
                     /locus_tag="PLD05_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOP76515.1"
                     /translation="MVSCELTDRELQNGPGVLDRAHDDLPSVPVRHQTPAATGSLSRL
                     LLTGIVVAFLGTFLVLPLIVVFVEAFRHGVTVYLAALTDRDALAAIRLTLFVAVAAIT
                     INTAFGLAAAWCLGKFRFPGRQLIIALIDLPISVSPVVVGLLLVLVYGRHGWLGSFLE
                     GHGLRIIFATPGIVLATAFVTLPLVVRELIPTMDAQGREQEEAARLLGANGWQVFLYV
                     TLPSIRWALLYGVILCTARAVGEFGAVSVVSGHIRGLTTTVPLHVEILYNEYRFSSAF
                     AVASLLTIVGVVSLAGKTLLERMARHRQMGIQHKAL"
     gene            23769..24854
                     /locus_tag="PLD05_03445"
     CDS             23769..24854
                     /locus_tag="PLD05_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HOP76516.1"
                     /translation="MQIDIIDVSKTFGRFVALDHIDLSISHGELVALLGPSGSGKTTL
                     LRIIAGLEWPDPGCGRLLFDGEDVTYQPAARRRIGFVFQHYALFRHMTVFDNVAFGLR
                     MQRRRLGLTKQEITDRVISLLRLVQLEHFRDRYPHQLSGGQRQRVALARALAVQPQVL
                     LLDEPFGALDAKVRRQLRTWLRKLHDEIGVTSIFVTHDQDEALELADRVVVMNEGRIE
                     QIGTPDEVYNHPKTPFVTEFIGSVNRFRGYRQGGHIRFGTTVVAEQGHEAVVFVRPHE
                     FTLSRFACGEGFWSRIVRSQMVGAQVRVWLEAESGEAFVAEIDRNQWDELEAGPGEQV
                     FVSPREIRCFDVQAASRVARSNRKVSA"
     gene            25023..26228
                     /locus_tag="PLD05_03450"
     CDS             25023..26228
                     /locus_tag="PLD05_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76517.1"
                     /translation="MAAFSSVSLRCRRPTVVWRRVLERQNGIRLCRFVVASVVLVGTF
                     LCGPRVLAQFFPPVVPPQPQERGRMGQFPPQMGRPGSVPGRNQPRGQGEQLPRIEASG
                     TVEVLTPAGLQIVSPSGQKWQLVLERECQVELTGKALPDVLRPGTVVSFTADIEKKTG
                     KATEKVASIAVCSIDQDHQLGVFPDQGLGGLPVGDGAAGGEMGGVGFGQGPNAFGLPG
                     GGFGSLGSPGEEPARPRRGRQNAQDEPPVERYQVCGRISTITKLGQITVVAPNPHFRA
                     PVQIELVENPEITLELSGRDALSLVRPGSKISGRGVQIGPTAARISELKVELVEPFTF
                     APPKKERPHAEEVEKPHPRPGVRTPSHRTPDEKASEGEKESPPTKETAPPDQSDPPKA
                     AGIELPPLP"
     gene            26249..27379
                     /locus_tag="PLD05_03455"
     CDS             26249..27379
                     /locus_tag="PLD05_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76518.1"
                     /translation="MSGGPSVASVQQSIGGDKPSQSFGAEVWQRLQRDRVARISLWIL
                     GTISVLALITPLLPLMPPRLVRTERQFQPPQFWPLFQHGFYDRPRWAASHSAEDEKQA
                     AEHLLSEGFGHLDPFSQALLRIRWMVFGEWSLAPICGTDKLGRDVFSRIMWGARVSLA
                     VGLVATLVSLLIGVTYGAIAGFVGGFLDRFMMRIVDILYSVPFIFLVIFIITIVSAEP
                     IRNFLETRWGLSRIIIFFFLVGAIYWLTMARVVRGQVISLKHELYVEAARALGASFWR
                     ILFRHIVPNLISIIVVYLTLTIPRVMLFEAFLSFLGLGVEPPDVSWGLLANEGLQAMT
                     PVRIYWWLIVFPGLALAATLFALNFLGDSLRDAIDPRLRNVR"
     gene            complement(27444..29147)
                     /locus_tag="PLD05_03460"
     CDS             complement(27444..29147)
                     /locus_tag="PLD05_03460"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF002727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOP76519.1"
                     /translation="MEREFTPGALRALAAAATWQLGETFDGLRPVTILMGLLAEPECR
                     AAALLRAIGIEETAILNRWPELRRSDRPPVITPESPPAFHAEAESILEAVDTYMDWGD
                     WPPIISTEHLLLGLVAGDEPVACWLREMGLEFHDLLRQILERYGTATKWRSPTVPLEL
                     SSSSEAQPEGEAFLPFDPEAAHRASPLSRQAPEITSSSESTPQRFDNREEKTRDSLGI
                     ATEMRLFRMVDAALNRAMEGFRVIEDYARFALDHPLLVREIKNIRHELAGLATAFPMP
                     VRLAARETQHDVGTEIKATTELHRMAVEDLVDANFSRVEEALRSIEECAKILAPQIVS
                     GIEQLRYRCYTVHRALRISETAAQRLQRVRLYVLVDARSSAEELRNLVGSLIDAGVHA
                     IQLREKHLCDRLLLERARLIREMTRGTNTLFIMNDRADLAVLSEADGVHVGQEELTVK
                     DARFIVGPRALVGVSTHNIEQARQAVLDGADYIGCGPTFPSTTKSFSEFAGLDFLRQV
                     AQEIRLPAFAIGGINEENLASVLATGIQRVAVSGCVIQAANPAEVARRLLARLERPSV
                     D"
     gene            complement(29296..31443)
                     /locus_tag="PLD05_03465"
     CDS             complement(29296..31443)
                     /locus_tag="PLD05_03465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOP76520.1"
                     /translation="MTLKEELIAPASDSAFDQLISSRLGVSAGLFTALKVRHAPTAVH
                     SLRVALVSSAWASFLKMEKEEQDRLELAALLHDVGMIGLPDQILLKPGPLTPEEMVYV
                     DRARFMTLEILRHVTDDQGLLRIVENVSAWFDGSRSGYVLSGRAIPEAARMIAIVEAY
                     DAMTTDHLYRPAMSEERAIAELYHFAGKQFDPDLVQDFVRFQREGLEASREAVAHRWL
                     SALDRTTVNQLWRWVSPARCIAPPELETVFQHRLLQWMRDGVIFVDTHKIILQWNHGA
                     ERLSGIMADAVRLRQWSPTILGFRNERGQPIDESDCPILTAINCGTQVLRRLTIKGRA
                     GKPVSVDVHVVPVILDDGVILGAVAILHDASSEISLEQRLEYFRDRVSKDPLTQLANR
                     AEFDRMLPLFVEQYQRRGLTFSLIICDLDRFKQVNDTYGHQAGDDAIRSLAAVFRSFY
                     RNGDLIARYGGEEFVMLCAGCDIATATRRAEEMRAALERTPQPRLNNRVVTASFGVTE
                     VQPGDTPETILRRADRALLMAKSRGRNVVVQLGAGNNGESIQKPVSRWWSWPHNSQEL
                     IWREDLISPIPLNLAMEKLHGFIADHLGRVVHLDRHHLEIEIEDRNPLRTRRSADRPI
                     CFGLRVDLSEVSTEVTQPSRTRLHLEVWPKSGRERRRREIVLRAHDLITSFHSYLMVS
                     EVTPEASATPWGRLKRLAARFLRTRPKTPTTDM"
     gene            complement(32248..>32784)
                     /locus_tag="PLD05_03470"
     CDS             complement(32248..>32784)
                     /locus_tag="PLD05_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76521.1"
                     /translation="MFQSIVEGRYVPWDLLVKDVPKDKHHDLDFIVSQLDWEKNVDPY
                     FKEHNYLEPIVDEENSGDGYTDRWIVYGLVDGKQLFSAKELTVMPGAKCVLRDPGASG
                     WITVQGCGKIGSLWLQTPIMIRFGEEPDDEVFITYEAAKAGVVVENTGTEPLVSLRYF
                     GPDVHPRVPNVGDYKKAR"
BASE COUNT         7065 a         9105 c         9202 g         7212 t
ORIGIN      
        1 ttggtgttac caggttgtac gggccggaag acgaagccca agggaagtgt tgaaggcaag
       61 gtcacgttta acgggcaccc ttatacggag gcctcggtcg tctttttcaa tcaggaaaca
      121 ggccaaggaa gttcggcgaa tatccaacct gatggttcct tcagacttcc cgaaaggatc
      181 gaggtcggca cctacatcgt gtatctggcg ccgaagatgg aagaggaccc cgcggcggaa
      241 ccgaagccag tctccattga taagacggtg ccggaaaagt actggagcga atcaacgagc
      301 gacatccggg ttgaggtaaa gacgggcagc aatcagttca atattgactt gaaaccttag
      361 catagcggct cggcctcggc cgggacatcg gtttctgttt gcacccgccc gcatccatcg
      421 tgaaggcatg tttccttcct gagcgatgaa tgggggcggg gtttctcatt attagggtca
      481 gagaattcgg gcacagggaa aacccctaaa aggcggttag agtccacctt ttcgcaaaat
      541 cttgccgaca aatgtcgttt tactcctttc cggaaagctc tcgccgacac gtctggcgat
      601 ccaagaagct tttaaatttc ctttatcagc tctgacgtct tccctttgca cgcttgcgat
      661 gaagagccgg accctgtatc ccacacctgt cggctaccaa aatcggcggg gtgaccatga
      721 tgtctttttt caccacctta ggcacgcaga ggtgccgaaa ctcgtcaacg ggatataacc
      781 taatggcgcc ttcccgccgg tattgggtgt ttcccgggca gccgagagcc tttcgtgcta
      841 gcccacgaag ccgctcgttt tgcccccaag cgctaggtgt cgtactgctt tgagagtggg
      901 acctcgacaa ataggattgc gatgaggtgt atggcattcc atttgcacac ggggaggaat
      961 cgagcaaaat gatcatccaa actgtcgaca gagggccccc gcggtgggtg cgtttatgca
     1021 cgacctgatg gaacttgtga ccttcattgt tgatcatacg cctgcccttt ggcaggcagc
     1081 cgacttcatc attcccgaaa acgcccttca aggctactgg gtcagtgcga ggagtcgttt
     1141 cgaacgttgg gcctcgacgc taaaggaact cacgtctcgc tcattgacag cctctcggga
     1201 atatcgtttt cggaaccaat tggctggcat tctggaagag atcctcacga atgaaattct
     1261 cacgcgggtg tggacggctg ctctttccct ttatgatcaa cagcggaagg tcgattgggc
     1321 agggccgatc ggtcaaagtg cttttctcgc tcagctagaa ctgcgtgtcc gttcgctggg
     1381 gattttaagc gatggccatt tcctcccggg ggaggatatc cagaaactcc tgcaactctg
     1441 tgaacggaat gaccgttggt gtgacctcct tctcagtcgc ctcagtgaat tcggtgatgt
     1501 ctcggcgtac gccgttgacc cacaacgatg gcaggagttc agcgcggact ttaaggccat
     1561 tcctacggaa aagcaacgca accaagcctg gcaattgatg cggacatcgc tcttggccgc
     1621 atatgccgtg cccatgttcc ggtatcacat gcaattgtcg cagacagtcc ttgtggcggg
     1681 atacattttg gattgcttcc ctcaagacgt ccttgagata tgtcaggatt tgaacttcta
     1741 ccgtgagtct cgttttgtgg cccgatgccg tcgcgcccag gagttgctgg cagaagtgnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ccgccggtcg taggcggcca
     1921 agatgaaatc acgacgaatg aggctaattg tctgctgccc cgcatgcgac ataacgacgg
     1981 gcagttcttc gaggtgtttt tcggccataa tttggagcac cgtgtgaagg tcatcgtcag
     2041 gggtgacaat gggcaccggc accgcgaggt cgatcgcccg caccaggttg cctgctccat
     2101 cgcccacgag cgccgcgcga agatcactca atgaaaagac ccccacaagc ttgttgtccc
     2161 gatcgacgac aggaaacgtc gtttgatgcg cgtcggccac agtacgaagg atctgcggca
     2221 acggtgtgtc ttctcgcagg acgatcggtt gtgtgctgaa agggcagacg tcacgcacgt
     2281 gcatctcttt gaacacgtca acgacaaagt cccctaaatg ggccgggcta tcgatgaagg
     2341 ctgtgacttg ctcccggcaa agggaaacgt ttccgggaag agagaccatt tccacagcac
     2401 ccaccagcat cattggcacg agcaaatcgt gggaacccgt gagctccgaa accataatca
     2461 gggctgctaa aggaactttg aatgtgcctg ccaggacacc gcccattccc acaagtgccg
     2521 tggctgaaac aggtggggct gaagccggaa acagcgcttg acaagccatt ccaaaggccg
     2581 cccctgtcat tgtccctata aaaagggcag gagcgaacaa accgccactt cctcccgacc
     2641 ccacggtaag agtcgttgcc accatcttca gaacgacaag gctgattgcc aaacgaaggg
     2701 atatgtcggg gcttgcgagg atggccttct ggatccatcc gtaaccacct gccatgacgt
     2761 gaggatagcc cacaaggacg atgataccca agccgagacc tcccagggcg ggtcgcagcc
     2821 actgcggcaa gcgagcgagc cggaagatac ggtctcgacc aaagtaaaac atgttaacaa
     2881 atatgtagga aactgctgcg cagagcaaag caagcgccag atagaagggc aattcatggg
     2941 cgccgtgaaa ggcgcacggc aaggccccgg tctggggatc aggggcagtt ggtaagacga
     3001 ataaaacggt ttgccccaga accgaggtaa aaatagtgta agcaacgaca gaagccacga
     3061 atgctggtgc cacggcggaa aactcaatgg ccgtcccgga ataaagaact tccaccgaga
     3121 acatcgctcc acctagcggc gtgcgaaaga ctgcccctaa tccaccggct gcccccgcca
     3181 gcatcagaat acgtcgatcc cagtctccta actttaggta ctgagccagc aatgaaccga
     3241 taccggcgcc gatctgtgtc atcggacctt cgcgaccagc agaaccgccc gtccctattg
     3301 tcacgatgct cgtaatcatt ttgatcaacg gtaccctagg tccgatgacc cctttcagtc
     3361 ggtgaaatgc ccgaatcatg gcatccgtgc catggccctc tgacgcaggc tccagaaaat
     3421 aaacgatcac accgcatagc aaacccccaa gcgtggggac caacagtact gcccaccatt
     3481 gccggggaag ctgaaagcgg gcgatgggat ccgccgtgtg ttccggaggt ggaacatagt
     3541 ccatgattct gcctaaggca ctatgctcaa caagctcaag agcgagcgta aaaagcacag
     3601 ccgccgcgcc ggcgcccacg ccaacaagca cactataacc cagcagtcgt accgacctgc
     3661 cccaaaccac catgtgacgg gaaaaaccgt tcaaggcgcg ccgaagtctt tgacccatca
     3721 tcaccaaccg accaaagcga tgttttgtcc cgcctccgtc ctcctcaccg tttggcgaag
     3781 acggaaaccg aacggcattc gaaacgttgg ttggacacat ggtgtgcaag tcacaaagct
     3841 gtttattaga cgtggatggt attcttagga tgtgatggaa acgtacttac aagattgatt
     3901 actttatttc ctcgacctgt actttttcct cgctggggca tgctagctcc gagaaaaaca
     3961 aagtatatac caccaacgcc gtgacgccgc aaaccaaaag gctgtcggca atgttgaagt
     4021 tcggccaggg ccaacgaccg atcattacta gaatccaatc tcggacggca tagactgggt
     4081 cacccacctg gtggagtccg ttggcctcat gccatcttag gccatgaaga ccgagacggt
     4141 cgtagagatt tccgaaaatc ccggcggtaa tggccgcaag gctcgtcgtc atccaccaac
     4201 tgtggccggc accaccacgg aaaaaccaca cgacaatccc cacggcgaac accacagaga
     4261 gggcggcaaa ccaacgcgtc atgccttgac ccatcccaaa gagcgccccc tcattaaggc
     4321 ttgtttgaaa gccgaaaaca tcgggaatca cccaccagac gggtttctcg ccgggcattc
     4381 ccagacgatt aaagatccac gcctttgtcg taaggtctgc cgccacgccc atcaccgtga
     4441 taaagaaaaa aacgcagatg cgggcgattg gtgctcgctt gaccactgat cattcctttc
     4501 taacgcttcc ccaaatcctt atcctcgcgg ctggacctca acacaacgat ctcttgatgg
     4561 ttctaaaaaa gcaccaagaa aatgtaaagg ggatttcctg gcaaaatgga acattcccta
     4621 agcaacattg ggacaatcgc accagcgctt cccaaaagtg cccgcgcaga cgctgccagg
     4681 ccgggaacca ccaaaggccc gcgtcggtta tcccacttgt ttgtccttcg gccgcaacaa
     4741 aaggaagttt cgaccctgta actgcacacg gtcgatcatg tcaaagcagc gggcaagtga
     4801 gatcggacga tcgtcgaagt tattggttcc caaggtaaat ttggccccta gctcccgcgc
     4861 caggcggata aacttctcgg gaggatactt gcccgcattg atctcgaggg cgataccccg
     4921 tttgacagcc gtctcaatga gtagccgcat ccgttcctcg gtccaaagct tgtcataaag
     4981 atgttcgatg ggtttgggca gccaggtcgg attggcgagg atggtaatcg gctccgagag
     5041 gacccgcaga ttgtgcttca tgtaccgttc catccacgcc tcgggatcgt cgatggtata
     5101 ttgatccgtc tgccacaatt tgaccggtgg actgttgtca tccggcatgg gcatgatcat
     5161 ggtatcggca agcacgtaat cgagctgatc gagaagttta ggatcgatca cctccgccca
     5221 atcacggtcg ttgacctgga tccccacgaa tacggggcgt cctgccacac tctccaagaa
     5281 agccgcaagt ttttcattat tgtccagggg ccaaccgcgc ccgtggtttt ccagcacccc
     5341 agcattgatg ccggtgacgg cctgtcgctc gatggccttc tccacggtca tcccgccccg
     5401 gaggtggata tggaaatcgg tagccggaat ggccgacatg gacaggcggt caatgaggcc
     5461 ctccgttatg ccgtagggtg agacatccgg tcgggggtcc gcaaaggggt ctgcttgatc
     5521 cggcaagggg cggatacgaa tgttacgaaa gtgaatgcga ctattgggat cgtgggcttg
     5581 cagggcaatc gtgccgcggg aaagccgtcg ccccacacga tttttcatcc gaggcgggga
     5641 ttctggttcg acatagtcca ccatcaagaa ctcattgacc cagacacgaa tccgcttgcc
     5701 gattaccagg atccgcatct ggaaccactg atcatccggc aaaaaggcct taaaaacgtc
     5761 ttggaccgca aacagtcccc ccgtgcggcg aaattccgga ttgtcgccca ccatgggata
     5821 agaattattg atctgaacct cataaccggc gcttggggaa ccggtctctt gatactgcgt
     5881 gtgaataaaa acgccggaat tggccccggg cttggtcagg acttcaagcg tgagatcgaa
     5941 attgcggaag ttgtggtcgc acacggggcc ttcgtaaaac aggtggctcc gtgggccgtc
     6001 ggccacaaga cagcctcctt ccacccgcca actgtctttg ttttccgatg cccgccatcc
     6061 ttccatcgag cgaccatcga acaagctgac cgaatcttcg gcgcaggccg tgactctgcc
     6121 acagccgacg aatccgaagg cagccagttg ggccgtgatc gccagaaacc cccgtcgtga
     6181 atgccgcaac gcctcgtgat ttgcagctcg ttcgttatgc cgtttcacag gatggttcct
     6241 ttatatgctg gtggggtaag acgaaaatat ctatccgagc tgtgcccggc ctatatgaac
     6301 gacaaacaat ctttctcact tcatcaagat gacatacaag caatcccgtg gcatttccag
     6361 agtcagacca tctccttgcc gttgcaaagg aaggtcctga cggggataac cgtccacatc
     6421 gagcaccaca gcccgcccta atccggaatt cttgacggtc atcttaattc ggttttctgc
     6481 caatcgccac ggtggccgac cggtatcgac gatctgtttg ccgcggagct tttggccgcc
     6541 cgattcaaac tctgcgtcgc gctccgtcca gcccgtcatt cgtgccgtag tacctacttg
     6601 gacaaggacc ttttcggaat cacgcaaggg acggttgtcc atcgtcacca ccatgatcgt
     6661 ggcgtaatcg ttttccgaca cgattgtcat gtcatcaaac gagaagcgac tacctccctg
     6721 tttcaggaaa cccgtgcaac cctggcactt gggagtgtta acggtgcaca cgccgttgcg
     6781 aaaatcgagc gagatttcgc ccgtcatact ccgcacgatt ccctttttgc gatcaataaa
     6841 cttggataga tccaacattc ggctattttt ttcatcgcct tcgaatttga catggactgg
     6901 tccgacaaag aatgccaggc gatcgacttc ctgtctgatc ggtgactggg gcgcgaaatc
     6961 cccggggtcg cggttgggat cgaatttgcc agcttctgct atgatgggca cgcgcctttc
     7021 ccacattccc tgaaggctcc gctcttcata gacggcgggc tgctccgcag ctcgaatgta
     7081 gctcaagcgg taggccagcg ctgccgcagg aaacattccc gcttgagtcg gtaccgaacc
     7141 ttcccatttc ttaagcgagt tcagtccccc gaccttccac cacggccaga cgggatcggc
     7201 cgtccagaat tcatccccat agctaaacca gtaaaaggca tcgacccccg tcaaagacat
     7261 atacacggca gccaggaacg gcccctctgt ctgataaagg gccggatgga cccaggccac
     7321 ttcggtcagg atcatcggct ggccgacagt ctgtttgacg gcgcccggaa attcctccgg
     7381 attgagtaaa cacgattggc tgatgtaata atggttggga tcgattcgcc agccgttcat
     7441 gggccccaca tgaatgacgc cggtgtagcg gttcaaggcc gccacgtcca tggccgtgta
     7501 ggtccagcgc tcacagtctt caaggagcac agggtcggcc gtccgccaat ttgttgcgtt
     7561 gagaagttgc ttgcagccca gcgtctcgcg atagaatttg gccatgtccg cgtaaaactg
     7621 atgttgaagt cggctcatga actcgagttg atcggcaagg cgcttggccc aggcagcgtt
     7681 gggctttggg gcctgcgatg tcatccgcca aatgatttcc atgccgactt cgcctgaggc
     7741 aaaatcgtct tgtttgtgac gggcatcccc ccaagcgtct tgggcctttt ccagcgaacc
     7801 gtatttctcg acaaggaatc ggccgaacag tcgccgcagt tttccggcga acggctcacg
     7861 gatattctgt gatgtccaga agaggatact gtcctcattc tgcacctgga taatagcgac
     7921 cgtagggtct tgagaaaggg gaatgcccgt gtaagggttg gggcgtgtgt ataactcttt
     7981 cacccaagtc ttgtaggcat cttgaagttt ttcctcaaag aaaatcaagc cctctggacc
     8041 ggactgccct gtgtaactgt ccagtcccca actggccggg attttgctgg tgtgagacca
     8101 atagggagaa atcgtgacat agattccgtg gtttttgcac tcggcaacgt atcgctgaat
     8161 ttgatcgatg actttttcgt ccacatcggt gagtttggcc ccttccttct ccacgcagag
     8221 ttgggcgtgc acgcggacca tgtttacccc caatttcgct agccaccgca agtgacgacg
     8281 cattacatcg ggttccatgc gccagccata atccacgacc gcccagaagc gaatcggttg
     8341 gccgtctccg cggagaaaac tcattccgtc ttcagaaagc cggatgaatc ccttttctcc
     8401 agccgtttgt tcgttcaaag agcgaagatc gaggagcgcc cccgcgcgga ttggatccac
     8461 actcggttca aagggccagg ttccaatctc tgtaacgaca tcggctcgag tctccgccgt
     8521 accgggacgt gccaggccgc tcggtgaaaa tggttcattc gtgagcacaa aggcatctaa
     8581 gcccccatga ttttgaagtt cactgtggaa gcgaaacgcc agcgtgtggt ctccgcgaga
     8641 taacgtcaac cttcccgcat taacccaacc aataaaacgg aggtcaattt tgttgtccgc
     8701 cgcaatgtta acaacttgcg tggccgcaga gaggtccaac tctcgccacg gcgaaccatc
     8761 gacgctcaaa gaaacgcgac ttctcacagg attgatgcgg agccagagga cgtaatcgcc
     8821 gccatccttc aggctcaccg aatactcgac cattccctct ttttcttcgg aaaaattgct
     8881 aatccaatcc ccacccgaga gatgttcctt tttgacctga tcgtaccacc aggggtgccg
     8941 gttcatcttg tggctaacag ggctttcacc ctcctgccag atggtttcac cccaggtcgg
     9001 cgaagaaatc cagcaagtaa ccagcgtgat taggatagtc aggaaatact tcatgatttg
     9061 ccgcctcatt ccgcaatcat gcattgcaag gactaccgta gattcacgcg gatcttccct
     9121 acggcgttat ctgatgtgtg aacgttcggt gctgcccagc tcaaaggttc tccgtaaagt
     9181 tgagcaaggc tgagaacatt cgcttttccg gggatacctt aattcaatct gggccattgt
     9241 aaagaacttg aggacctcaa ggaagtacac cgcggaccgc acggctgaac cgcacagttg
     9301 accgcccaaa aaagccccgt tacgctggtt gcatctaggg gctttggaaa gccatcacca
     9361 ggtgaccaca acgcacgaac gacgttaaag ttcatgctgt ctgattcgag gagctgagaa
     9421 atgggcgagg cgcggatcgt ctcctttatc aactacaaag gcggagtggg caagacgacc
     9481 acgacttatc acatcgggtg tagtttagcc gagcatcatg gtcatcgcgt gttgctggtc
     9541 gatattgatc ctcagtcgaa tctttcgctg ctttgtatga gctatgaccg gtacaatcaa
     9601 tttcggaccc aacagggcac aattcgcgat ctttatcgtc gatttcggca ggagagccgc
     9661 ctgggtacac gggatttcat cgtccgcgag ccgattcagt tgaacaacgg tccgctgagg
     9721 aatctcgatc tgctcccgtg tgatctggat atgctgggcg aagatattgg gggaatactg
     9781 ccgactgttt ctgccgcgcg gacggggaac gcatttcagg tcttggaaca ggtggcggag
     9841 cgcgtgttgc gagaatggga ctttttgcgt ctggcacttg atgaagtccg cagcgagtat
     9901 gacgatatcc tcatcgattg cccgcccaat ctctatttga tgacccagaa tgccctggcc
     9961 gcctctgact ggtatgtcat taccgtggtt ccggaacacc tttcgtcggt tggggtgccc
    10021 atcctcattc gtaagatacg agatatccgc gagcggattc ggcgagtggc ggccatcctt
    10081 gggagagagg tttcgtttgc ccggtttgcc ggtattattg ctgttaaaat gcgtcgggca
    10141 ggccagcatc aggacgtgct caaccggctg cgtgaggatc tatcgagtta tattggcgag
    10201 ggtagtttgg ctgatccagt tttcgctaca gccaccacag aattgatcgg ctacagcgag
    10261 gcagcggagc agaatctccc cgtgtggcat gcgcggggaa acaatccagc catggcggcg
    10321 gaacggcggg aatacgaagc cattaccgaa gaatacctga gacgctgtca tagcggtgag
    10381 ctatgagaca gtggcaatga aataaaagga cgcccttttc cagggcgctt cgtaggagtg
    10441 tttcccaatg tcaatgtgaa aggatggatt atggccaaaa ggcagtcagc ttcgacgtta
    10501 gaggtgcttg aagagcttag tggcgttttt aacttgttac cgactgagga gttccggaac
    10561 gaagtcacta catcggtgca gcagattata gggtttttgc aggatttgcg tgttgcgatt
    10621 gaaaagatcc ccacccggga acagatgata gataggcgta ttcctgagtc acttgatcgc
    10681 gtcagggatt tcttgaagcc cttcgagtcg gccggcagag ccagatcgtc gcgtcaagcg
    10741 cagcggagtg tcaaaaaagt agtcgatctc gaggagttca aagaccatct gatgaccctt
    10801 cccattgggc agattgaagg cgcactgcgg gattcaggat tgacattgga gcaactgaaa
    10861 gaggtgttaa cgcgtctcgg gggttctagt cgtggtgcta ggagtaaaga gcaggttatc
    10921 cagcggatcg tggatgagat tcgtactcgg cgggaactga gtggcttacg ggacggggcc
    10981 accccttaaa accagtaaga tcaatacctg tttgaccagg gaaatatgca atcacggtgg
    11041 ggacatcgca ccattgcgtg tccccaccgt atctgatttt tacggagcta aaagctggtt
    11101 gccggcgtca ctcgatgagt tctggggcga attcacggcg tagtacggcg agggcctgcc
    11161 gtcccgtatc gccgcttacc acgacattca ggcgaacttc gctggtactg atcatttcca
    11221 cattgatttg ggcagcagcg agggcctgga aggtgcgacc ggccacacta atgtggctct
    11281 taagcccggt accgaggatc gacagtttgg caacactcgg gcgacccgtg ggtggtgcgg
    11341 ccccaatctc ttcggcaaag gcctggcacg ctgtcatggc ctcctcgtac gacccttgtg
    11401 gtaccgtgaa gctcatcatg gctttatctt ctcgccccac gctttgcacg atcatatcga
    11461 cgacgatgtt cctatccgcc acctggcgaa acagcttggc agcaaggccg gggcgatcgg
    11521 gaatctcgct aagcgtgaca catccctggg tttcgtccaa acaaatcgac tcgatgacaa
    11581 ggtcctccat accgtgaagg cgaccgataa tccgttcgcg gtcgttggcg acgacctcag
    11641 ggcggctttc ctcggccaca ctcggcggtt ccttgtgcag ctcaaaggct tggtggaccg
    11701 tgcgtaaagc ctccacggcc tgatcccgtt gcaccagcac ggaaatcttg atctcactgg
    11761 tcgtgatcat gaggatattg attcccttat cggccaaggc ccggaacatc cgttgggcca
    11821 cgcccggttg tttggccatt cccaaaccaa ccaccgagac cttggcaaca ttttcatcat
    11881 aggtcacacc ctcggcgccg atttcgcgag ctgtctcttc cacaacccgc aaggcggcgg
    11941 gaagctcctc ctgaaacacc gtgaaggaga tatccgcccg accgcctgcc gccacgtttt
    12001 gcacgatcat gtccatggga atattccggg cagccaaacg cgagaaaata acggctgcat
    12061 tgccgggttt atcgggcaca ccacgaacgg tgatgcgtgc ttccttaagg atcgcggccg
    12121 ccccgcacac aggaacgtcc cgcgattctg gcgtccacga aatgatggtt cccggcaagt
    12181 cgctgaaact actccgtacg tggacgggca ctgagaaccg cttggcaaac tcgatggagc
    12241 gattgtgcat caccccggcc cccgccgtcg ccaattcgag catctcgtca taactgatgc
    12301 ggctcattcg gcgggcttct gggaccagtc ggggatcggt cgtatagacg ccatcgacat
    12361 ccgtgtaaat ctcgcaggtt tccgcccgta acgccgcagc tagggcaacc gccgtggtgt
    12421 cgcttccccc acgtcccagg gtcgtaatat cggcggtgtc ggtgatccct tggaatcctg
    12481 ccgcaataac aatattgccc tcgtcgagca gttgtcgaat ccggtctgta gagatggatc
    12541 gaatgcgggc cttggtgtgg gtactatccg tgtaaatgcc catctgggcc cccgtcatac
    12601 tgatggcccg atgccctagg cggtggattg ccatggccat gagggcaacg ctgacttgct
    12661 cgccagtcga aagcagcata tccatttccc gggccggagg ctcagggtcg atttgcttcg
    12721 ccaattcgat tagatgatcg ctagtgtgac ccattgcgct gacaacggtc acaacctgat
    12781 aaccctgaag cttggcccga atggcttttc gggcagccgc taggattttt tctgcatcgg
    12841 cgacacttgt cccaccaaac ttttgaacta acactgccat tgtgcgcaat ccttgttcca
    12901 atctttgact caactgtttc taactgcgta ttccaaaaag gaatctcttt cacccaatca
    12961 agtaccctgc tggtggtcac gaacctgtcg tgagcagcca ctcaaggtgg cgattcaccc
    13021 cgaaggaaat actccatcac acgccaaccc tcttcaaaga taatccccga ttcttccgcc
    13081 gtccgctcgt tgtagcccgt cgcgccactg gtgggaatcc ccgtcccagc aatcgcaaag
    13141 ggaacgtagc cgtgggcgtg agttttcaac ctgagtggcg taggatgatc gggtgaaaca
    13201 agaatcctat aatccccata actttgaagg gcctcccaca agggggccac gattttctga
    13261 tcgatcgcct ctagggcttg gatttttgcc tcggcgtcac cctcgtgcga ggcctcgtca
    13321 gtggcttcga tgtgaacgca tactaggtcc acttctttta gggcttcaat agcgtagcgt
    13381 cccttggcgg cgtaatctgt atcgatgtaa ccggttgctc caggaacatc aatccgtcgc
    13441 catccgaaca aggcggccag gccgcgaaga aggtcaaccg ccgtgatcat cgccccggtg
    13501 accccatatc gttgttgaaa cggcggtaac tgcggggctc ccccaagtcc ccacagccag
    13561 atattcgtag ccggcagctt tcctgccttt tgccgggcca cattgactgg gtggttggca
    13621 aagatttccg cgctttcgca cattaaccga gtcaacaagt cactgccggg accacgggga
    13681 taagcgtcaa tcacgctttg atcggtcagg tcgtgagggg gaacggtcac ggtgtcacgc
    13741 gaaaacggcg ccggcttgtc cccacctcga tagattaaaa ggttacgata gctcacacca
    13801 ggaaagaact ggagttgggg cgttcccagc ttttcctgaa gggtggtcat caactcgcgg
    13861 gcttccccgg ttgagatgtg gtcggccgta aaatctcgca tcacctgatc gatgatcgtg
    13921 accaggttgc agcggatcgc ccaatcattg gggccgagag gaatgccctg ggcggctgcc
    13981 tccagtggtg cccgacctgt gtaatgcgca accggatcgt agccgagaag actcagattg
    14041 gccacatccg agcccggcgg cagggagcgt ggaacgtgat tggcctggcc gacaacccca
    14101 gcgcgggcaa cagcatccat ggccggaata tcagcggcct caaggggagt ctttcccccg
    14161 aggctttcct ggggttcgtc ggcgcagcca tcgggaataa caattgcgta ttttctcata
    14221 tcgcttgaac cattgtctgg tagatttact acgaggaagc tcaggaatta aaggttcaca
    14281 gccacaaaac tcctatcaat cccgcacccg catccacaca ctgcccggac gaaccaccgg
    14341 caatctgtca atctcagcca tcgccttccg catggggcct tccgccgcgg tatgagtcat
    14401 gatcaccaat ggcacaacgc cttccgtctc ttcttctgtc tgatgttgga tgacagatgc
    14461 gatggaaatc tggtttcgac ccaagatgcc ggcgatctcg gccaggacac ccggccgatc
    14521 ttgaacatgg aagcgaaggt aaaaccgact tcgggccata tccgggttac gcaagccaat
    14581 gtggggaccg tcgttgctcc acaatcgcag cgtgcggaat gtgatggccg cccgaccaac
    14641 cgcagtgtcg atgatatcgg ctaccaccgc cgaggcggtc ggcatttggc cagctcccaa
    14701 gccatggaaa aagacccgac caacggcatc acccacgacg cggacggcat tgtatgcccc
    14761 gcggacctca ccaaggggtg tcccctcccg gaccaatgta ggcgatacgt gcaactcaag
    14821 ctcaccgtcg attagttcag ctacggccaa caggcgaatg tgatacccaa gctctcgagc
    14881 ataccgcatg tcctccgcct ggacggtgtc gatccccacc cgcgggatat ccctccaatt
    14941 aatttgggca ccgaaggcga gctgagcgag aattgcgagt ttttgggccg cgtcggcacc
    15001 gcttacatcc atggaaggat ttgcctcggc gtagcccagt cgctgggcct ccgccaaagc
    15061 ggcggcgtac tcctgaccac gttcctccat ctgagtcagg ataaagttac tggtaccatt
    15121 aaggatggcg tggatcgagg taatttcgtt ggcggtcagg ctttcgccga tagccgcaat
    15181 gatcggaatc ccgcccgcta ccgcggcctc gaaggatatc gaacgcccca aacgtcgtgc
    15241 agcctcaaat agctcggggc cgtgctccgc caatagggcc ttgttggccg taaccacgtc
    15301 tttgccgtgc tccaaaagcg acaggacaat ggaacgcgtt ggttccacgc cgccgatcag
    15361 ttcgaccgcc acggcaatgt tggggtcttt gattacggcg tccaaatcgt cggtcaaaag
    15421 atgctttggc accgtgacgt tgcggggccg gacaaggtcc ttgtccacaa tccggacaag
    15481 ctcgatcggc cgccccgccc gccgagcaat ccggtcaccg tgttccaata gcaattttgc
    15541 gacaccagag ccgatggttc cgaagccgat aatagcgact ttaaggctgt ccatattggg
    15601 tgcctattcc catgattgcc acgaaaggcc acttttccga taatcatctt cgacaaaatg
    15661 gaacgccttg ccaggcatgg gcggcctgtt ctgctaaaag ctgtaaaccg ttatcctagg
    15721 ttgacgctcg aaaagccgtc aagatgttcc cggccgcgga agggcctttg ccgcttggtt
    15781 ggttgtacgc catcctttaa gccagcgcaa gaatgaaacg acttttggaa cagatgctct
    15841 cttagatcag atttccgttt acggggaggt ctcagatgaa aacattggtg actatgatgt
    15901 gccgggacgt gtacctgacc tgggtttcgt ggttgttaat gctgacctta ttggcttcgc
    15961 tgggacttgt tgaggcaagc caggggagtg gtgcggaacg ccccaatgtc gtgttggtga
    16021 ttacggatga tcagggctat ggcgatttgg cctgccatgg taatccggtc atcaagaccc
    16081 ccaatttaga tcggctctac caggaaagcg tgcggctgac tgattttcac gtcgatccca
    16141 cgtgctcgcc cacccgtgcg gccttgatga caggccgtta ctcctgccga accggggttt
    16201 ggcatacgat catgggccgt tctattttgc ggcgagatga gacgaccatg gcggatgtct
    16261 ttcgccgtgc ggggtaccga acggctatct ttggaaaatg gcacctcgga gacaattacc
    16321 cctatcaccc atggaaccgg ggatttgagg aatgcctcat ccatttcggg ggtggcatcg
    16381 gtcagggacc ggactattgg ggaaacacat acttctcgcc aacactcaac cataatggtg
    16441 aatggaagaa ggaggaaggt tactgtacgg acatcttctt taaaagggcc atggagttca
    16501 tccaggcgca ccgcgaagaa ccgttttttg tctatcttcc cacgaatgcg cctcatgccc
    16561 cgcttcaggt tgaagagcgc tatgctgaac cgtatcgcca agccgggctg cctgacgctt
    16621 tggcaaaatt ctatgggatg attaccaaca tcgacgagaa tatggggcga ctaatggggc
    16681 tattgcgaga attgaaactg gaatcccaaa caattctcat cttcatgacg gataatggga
    16741 ccgccgggag tggtttcaac gccggaatgc ggggaaagaa aggctcccaa tacgacggtg
    16801 gtcatcgcgt cccctgtttc atccgctggc caggacgtct cccggctggc caagacttca
    16861 cccacttggc ggcacacatc gaccttttcc ccacactgct tgatctgtgc gacatcccgg
    16921 cccccagcga agtgaaattt gacgggataa gcctcaaacc gtatcttgcc ggcggaactg
    16981 ccacgtttcc cgagcggaca ttattcgtcc aggttaaccg tattgatcat ccggttccct
    17041 ggcgattgtc tgccgttctg actgagcgat accggttgat tgatggtcaa cagctttacg
    17101 acatcaagga ggatcccgga caaacaaagg atattgcggc ggccaatgcc gaggtcgtga
    17161 atgagcttcg ggcgaggtac gaagcctggt acgcggacgt ctcccagcga tttggcgagt
    17221 attgcgaatt ggtgctcgga tccgacgagg aaaacccggc cacgttgtgt tcccacgatt
    17281 ggcacggcga atcggttcca tgggaccagt cgcagatgga gaaggggatg gtgggcaacg
    17341 gcttttgggc tgtggaattc gccaaggccg ggcgatatcg ttttgccctc cgcgatcgcc
    17401 cggcccacgc ttccaccaag ttgccccagg gaaaggcccg cttggtcgtg gaaggactgt
    17461 ttgaagaaga aaagccaatc ccagccaatg ccgaagaaat catctttgag gccaatttgc
    17521 ctgcggggaa ggcccgcctg caaacattcc taacgcttcc taatgggacc cagcgaggct
    17581 tctatttcgt ggtcgttact cgactacctt gacagccgtt gccgagtgtt caaactgcaa
    17641 ggtgtcacgg ctggtgagct taagcgtccc ccgggacatg ggaaggctgg ggatcattgg
    17701 ctgactgttg cgattgggcc acatcttgcg agagccgcat cgagcaaaac ttaggcccac
    17761 acatgctgca atatttggca ttgggcgacg taccagggcg ggcggtctct tcccacattt
    17821 gccgtgccct ttccggatcg atggccaatc ggaattgctc gttccaatca aaccggtaac
    17881 gggcgcggct taaggcgtcg tcccagtcgc gggcacctgg tcgatgccgt gccacatcgg
    17941 cagcgtgggc cgctatcttg tacgccacaa cgccttgacg gacatcctcc agtgtgggca
    18001 ggcccaggtg ttctcgaggt gtgacataac agagcatggc ggccccgtac caagcggcca
    18061 tggcagcccc aatggcgctt gagatgtggt cgtatccggg ggcgacgtcg gtaaccaccg
    18121 gtcccaggac gtaaaacggg gcctcgtcgc accactccat ttggagccgg atgttccgct
    18181 caatctggtc catggggatg tgtcccggcc cttcgatcat gacctggcag cccctctcac
    18241 gggcccggcg accgagttca cccatgactt tcaactccgc gaattgggcc tcatcgctgg
    18301 catcagcaag acaccctggc cgaagcccgt ctccgaggct ccacgtgaca tcatattgcc
    18361 gcatgatgtc gcagaggtca tcaaagtggg tgtagagggg gttttcggcc tggtggacgg
    18421 tcatccagcg ggcgatcaag gccccgccgc ggctgacaat gcccgtcact cgccgcgata
    18481 ccaaaggcag atgctcccgt agtagggcgc agtgaatcgt catgtaatcg acgccctggg
    18541 ttgcctgctg ttccaccaca tccaagaaat gctgcggggt catctcggca atgtccccga
    18601 gttcttcggc aacctggtaa atagggactg tccccacggg cacaggcgag gcatcgatga
    18661 ttcgctgccg aatcgccgtg atgtccggac ccgtggagag gtccatcacg gtatcggcgc
    18721 ccaagctaat cgcacaaaag accttttcca gttcacacgc cacatcactt tgaagggccg
    18781 agttgcccaa attggcattg atcttcgttc ggcatgcgat ccctatcccg atggggcgca
    18841 gatccttctt aaggtgaatc ttgttggccg gtatcaccat tcgcccgcgg gcaacttcat
    18901 cccgaacggt ttccaccggg aggtgctccg atttggcaac gtattccatt tcgggtgtga
    18961 taatccccgc ccgggcggct tcaatctgag ttgacatttt ttcgacgatt ccctgacttc
    19021 taaactcggt taacaacgaa accctcttcg gttaacggca agcagggccg actctttaat
    19081 catattggat tccccaaaac gaacaaccaa gcacacagga agtttgctga ttccatcttt
    19141 gggattttgg aaggcgtttt gtgggacgcg aaagctggtc ttgccgtaag tcccttcaac
    19201 gtgagggttt gttttaagat gatcagatag agattcatga ccttgaaacc ccgttctcgg
    19261 tacggaattt ggcccagcag attcccatct ttctcatgcg tgatcgcagg gtgtggggat
    19321 tgattcccaa tagggcggcc gccccgcggg gcccttcgat ccgacccgca caacgccgca
    19381 atgcctcctc gatgtgcctt cgcatcacct catccagtgt aggaaagtca ccctcctgtg
    19441 gctgcagagt ctccgtcttg gacacgctgg gcaccgcggt gacgcccagg gccttcagga
    19501 cttccagccc gcgaccgttg ccgagaattg cagcgcggtc gatgaccgcg gccaattcac
    19561 ggatattgcc cggccagtgg taggaaagca acgcccgaat atcctcctcg gtgggtagct
    19621 gcaacggaag cccaaaccgc acagcggccc gctgggcaaa atgtcgggct aaggcgggga
    19681 tatcctcccg tcgctcccgg aggggaggca aatagatggg gaagacggcc aaccggtacc
    19741 agaggtcttc tcggaattgg ccctggcgaa ccatctcctg taagtcgcga tgagtggcgg
    19801 ccacgacccg cacgtcaacg gtgatcggat ggcaccctcc cactcgctcg aaagtcccgt
    19861 cttgaagcac ctgaagcaag cggacttggg catcgagggg taattccccg atctcgtcca
    19921 agaataacgt cccgtgattg gctcgctcga accacccttg atgggtttct accgccccgg
    19981 taaagctacc ccgttcgtga ccaaagagtt gtgagtcgat caattcccga ggaatcgcgc
    20041 cgcagttcac acggagaaag ggccctttag cgcgggggga ccgttggtgg atggttcggg
    20101 caatcagttc cttgcccgtg cctgtctctc ccagaagaag aacgggcgca tccgacatcg
    20161 ccaccagttc cacccgctcc ataacgtgtt ttagccccgt ctcggccccg ataatagcat
    20221 cgctaagcga ttgccgaccg agtttcgcca gcagtgaccg cttttctgcc tcggcagctt
    20281 ctcgcagggt ggccatctcg cgcaatcgtt ggtcattttc cagagcaacg gcaaacggtt
    20341 cgagaagttg ctggaccaga cgaacatctt cctcggagaa ggccgcccgg ttgggttccc
    20401 ccacgaccgc taggatcccc gcgggcgttt gtccagccaa caagccaccg atgagcatcg
    20461 gggcgtggat ttcgccaaac agttcgtggg catcgagccc atcaaatgcc ccatcttggg
    20521 ggatattcca tataacgcgc cgagaaaacc aatcaagaag ccgggacatg gataccctgg
    20581 agaggggtgt tgttcggggg cccaagcccg gtgggctctt cggagccacg gctacggttt
    20641 gcaactgcat ctggctgagg tcgagtcgcc gcacgacgag tgtctgaaac cgcatttggg
    20701 cggacaaaac ttgggcgatg gccgccgccg actccgtgat ctcgatatgc cggcaggctt
    20761 cccgccagat gctcacaagc gttgtctgaa agtccatgga aaggggccct tttgcaatcg
    20821 aatctcgctc gactcgggga atctagcagc gtgcccatta tatttcacgg cgagccgtta
    20881 ggctagacgg atccgcgccg tgatatttca cggcnnnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnngccaag gattggtttg aaaaaacggg cgagacggtg gagattcggc
    21061 agtctcatgg tggttctggc aagcaagcac gagcggtgat ggaggggctc gaggcggatg
    21121 tcgtcaccct agccctggct tatgacatcg acagtatcgc tacgcggacg caactgatcg
    21181 accaaaactg gcaggaccgt ctgccccatc gaagctgtcc ctatgtttcg acgatcgttt
    21241 ttctcgtgcg aaagggaaat cctaaaaaca tccgggactg gaacgacctg acgcgaccgg
    21301 atgtggaagt cgtaacgccg aatcctaaga cctcaggcgg ggcgcgctgg aattatctcg
    21361 cagcctgggg gtacgcccta agacgatcgc ttggcgatct gaaaaaggcc aaggacttga
    21421 attcgcccgc ggttgcccag gcccagaaag aagccaagga gtttgttcgg caaatcttcg
    21481 cccgggttaa ggtcatggat accggagcca gggccgcaac gaacacgttc gcccaaaggg
    21541 gtatcggcga cgtgctgata gcctgggaga atgaggccct gctgacggtc aaggaactgg
    21601 gggcggacaa gtttgagatt gtcgtgccca gtgtcagcat ccttgccgaa ccgccagtag
    21661 ccgtcgtgga tcaagttgtc gatcgccggg gaacgaggca ggtggcagag gcttatcttc
    21721 agttcctttt tgggatcgag gcacagcgga tcattgcgcg acattattat cgaccgactc
    21781 tgcctgaggt agcccaggag ttcgcagggc aattccctca attggaacta ttcacaattg
    21841 atgatgtctt tgggggttgg caaaccgctc agaaagagca ctttgatgat ggcggtcttt
    21901 ttgaccaaat catggcagcg acacggtgag ataaggtgat gaccggtagg atcgatcaat
    21961 acggctttcg cggccttttt cgggatgatg tcttgccggg cttttggctg acattcggga
    22021 tcactattgc ctacctggga gtgatggtcc ttctgccctt tgccgctttg gtcgcttttg
    22081 tgacgggtag tgccgacggc tggcaagtct tgcaagagtc aagagtctgg gcagccgtcc
    22141 gcttgagtgt gggtggcgct ctggtggctg ccttgattga tgccgttttg ggcgtgctga
    22201 cggcctgggt ccttgtgcgg tataggtttc cagggcggca attgctagat gccttggtcg
    22261 atttgccgtt tgcccttccc acggccgtct cggggattgc gctgaccacg ttgtttgccc
    22321 cgaagggatg gctgggagct tggctacaac ctttggggat tcaggtggcc tacacgcaac
    22381 ttgggattct cgtcgcgctg gtgttcatcg gtttaccgtt tgtggttcga gccgttcagc
    22441 cggccatcga ggatttggac ccggaatggg aagaagctgc ggccagtttg ggtgcttcaa
    22501 gatgggcaac cttttggcgg gtcatatttc ctagcctttg gccctccgtg ttgacggggt
    22561 ttgcgatggc ctttgcacgt ggggtgggcg aatatgggtc ggtgatcttc attgctggga
    22621 atctccccca tcgcacagag atcgctccgc ttttgatcgt gattaagttg gagcagtacg
    22681 actatcacgg ggcggccgtg attgcgacga tcatgttgtt ggtttccctt ggggtgctgc
    22741 ttgccgtggg agccttccag aattggagtc agccatggca aaaggccgca acacattgag
    22801 cagataaacc atggtttcat gcgaattgac agatcgagag ttacaaaacg gtccgggtgt
    22861 cctggatagg gcccacgatg acttaccgag tgtgcctgtt cgtcaccaaa caccggccgc
    22921 cacggggtct ttgtcgcggc tattgttgac gggcatcgtc gttgcgttct tgggaacctt
    22981 tttggtattg ccgttgatcg tggtctttgt ggaggccttt cgtcacggtg tcaccgtcta
    23041 tcttgccgcg ctcaccgatc gcgatgccct tgcggccatt aggttgacgc tgttcgtagc
    23101 cgtagcggcg atcacaatca acacggcctt tggattggcg gcagcctggt gtttggggaa
    23161 gtttcgtttt cccgggcggc aattgatcat cgcccttatt gatttgccga tttctgtttc
    23221 gcccgtagtg gttgggctac tgctggtcct cgtttatggt cgtcacggct ggttgggtag
    23281 tttcttggaa gggcacggcc tgcggatcat ttttgctacg ccgggcattg tcttggctac
    23341 ggccttcgtc acgttgccgc ttgtagttcg ggaattgatc cccacgatgg atgcccaagg
    23401 acgcgaacag gaggaggccg cccggctcct tggtgccaat ggatggcagg tctttctgta
    23461 cgtgacactg cccagcattc ggtgggcttt gttgtacggg gtgattttgt gcacggcgcg
    23521 ggccgtgggc gaatttgggg ccgtctcggt ggtctcggga cacattcggg gtcttacgac
    23581 gaccgtcccg cttcacgttg agattcttta caacgaatac cgattttcat cggcttttgc
    23641 tgtggcctcc ctgctgacga ttgttggggt ggtgagccta gcgggaaaga cccttttgga
    23701 acgaatggcg cggcaccggc aaatgggaat ccaacacaaa gccttgtaac cagaatggga
    23761 acatcgctat gcaaatcgac atcattgatg tttcaaagac atttggacga tttgtcgccc
    23821 tcgatcatat tgatctgtcg atttcccacg gcgaactggt ggcgctactg ggtccttcgg
    23881 gctcgggcaa gacaacgctc cttcgaatca tcgctggttt agagtggcct gatcctgggt
    23941 gtggacgcct cctttttgat ggtgaagacg tgacctatca gccggctgct cggcggcgga
    24001 ttggatttgt ctttcagcat tatgcccttt ttcggcacat gaccgttttc gacaatgttg
    24061 cctttggact gcggatgcag cgccggcggc ttgggctgac aaagcaagaa attaccgatc
    24121 gcgtgataag tctgctccgt cttgttcaac tggaacactt ccgcgaccgt tatccgcatc
    24181 agctttccgg ggggcaaagg cagcgggtgg cattggcgcg ggcgctagcc gttcaacccc
    24241 aggtcttgct cctcgacgag ccgttcgggg cacttgatgc caaggttcgg cggcagcttc
    24301 ggacatggtt gcgtaagctt cacgatgaga ttggtgtgac cagcatcttc gtcacgcatg
    24361 accaggatga ggccctggaa ttggccgacc gcgttgtggt catgaatgaa gggcgcatcg
    24421 agcagatcgg gacgcccgac gaggtttata accatcccaa gacgccgttt gtgaccgagt
    24481 tcattgggtc ggtcaatcgt tttcggggtt atcgccaggg gggacacatc cgctttggaa
    24541 ccaccgtggt cgcggaacag ggacacgaag ccgtcgtttt cgttcgcccg cacgaattca
    24601 ctttgagccg gttcgcctgt ggcgaaggct tttggtcgcg gattgtgcgg agccaaatgg
    24661 tgggggcaca ggtgcgagtt tggttggagg cagaatctgg cgaggctttt gtggccgaaa
    24721 tcgaccgcaa tcaatgggac gaactggagg cgggtcccgg tgagcaggtg ttcgtttcac
    24781 ctcgtgaaat ccgctgcttt gacgtacaag cagcatctcg ggtggcgcga tcaaatcgga
    24841 aagtgtccgc ttgaggtggc ggcttcgcct ttccgaaagt cggcgtctgc cctgtcacgc
    24901 ggaagatggg tgtgccttcg cgaaagcttt tgtggcgaga actggcaaac gcagattcgt
    24961 ttcggtatgc tgaaggtggt gaactctagc cagctgctgt cctaaattgg ggaaacgaag
    25021 ttatggccgc cttctcaagt gtctcgttgc gttgccgtcg tccaactgtg gtttggcgac
    25081 gggtgctgga acgccagaat ggcattcggt tatgtcggtt tgttgtcgcg tcagtggtgt
    25141 tggtggggac cttcttgtgc gggccaaggg tgcttgccca gttctttccg ccggtagtcc
    25201 cacctcaacc gcaggaacgt gggcgtatgg gccagtttcc acctcagatg ggacgtccag
    25261 ggagcgttcc cggccgaaat cagccacggg ggcagggcga acaactccct aggatcgagg
    25321 cttccggaac ggttgaggtt ctgacgccgg caggcctgca gatcgtttcg ccttctggcc
    25381 aaaaatggca gcttgttttg gaacgtgagt gccaggttga actgacgggg aaggcattgc
    25441 ccgatgtcct tcgaccgggc acggtggtga gttttaccgc cgatatagaa aagaaaaccg
    25501 ggaaggccac ggagaaagtg gcgtcgatcg cagtttgttc catcgatcaa gatcaccagt
    25561 taggggtgtt tccagatcaa ggactgggtg gtcttcccgt gggtgatggg gcagccggtg
    25621 gtgagatggg tggcgtgggc ttcggtcagg gcccgaatgc gttcggtctc cccgggggcg
    25681 gcttcggcag tttgggtagt cctggggaag agcctgcgcg gcctcggcga ggacgacaga
    25741 atgcccagga tgaaccgccg gtagaacgtt accaggtctg cggacgaatt tccaccatca
    25801 cgaagttggg tcaaatcacg gtcgtagcac ccaatccgca cttccgtgcc cccgttcaaa
    25861 ttgagcttgt cgaaaacccc gagattactc tggaactttc cggtcgggac gcgctctccc
    25921 tcgttcgacc gggttccaag attagtggca ggggtgtgca gattggtccg acggcggcac
    25981 gtatatccga gttaaaggtg gaacttgtcg aaccattcac gtttgcaccg cccaagaagg
    26041 aaaggccaca cgcggaggaa gtggaaaagc cgcatccgcg gccgggagtt cgaacgccca
    26101 gccatcgtac gccagacgaa aaggcaagtg aaggagagaa ggagtcgcca ccaacaaaag
    26161 aaacagcccc gccggatcag agtgatccgc caaaggcagc cggcattgaa cttccaccct
    26221 tgccttgaga tagcatcctc tcctagcgat gagtggtgga ccgtctgtag cgtccgtcca
    26281 acaatccatc ggcggggata agccgtcgca atcctttggc gccgaggtgt ggcaacgttt
    26341 gcagcgggat cgtgttgccc gcatttccct ttggatcctt gggaccattt ccgtcttggc
    26401 cctgataacg ccattgttgc cactcatgcc acctcgcctg gtccggaccg agcggcaatt
    26461 ccaaccgcca cagttctggc cgctgttcca gcatggattt tacgacagac cccgatgggc
    26521 ggcatcacac tccgcagaag atgaaaaaca ggctgccgag cacttgctgt cggaaggctt
    26581 tgggcatcta gacccgttca gtcaagccct ccttcgtatc cgctggatgg tctttgggga
    26641 atggtccctg gcccccattt gcgggaccga caagctgggg cgagacgttt tctcccggat
    26701 tatgtggggg gcacgcgtat cgttagcggt gggccttgtg gctaccttgg tctcgctcct
    26761 tatcggcgtg acctatggcg cgattgccgg ttttgtgggc ggtttcttgg accgcttcat
    26821 gatgcgtatt gtggatatcc tctattctgt cccatttatt tttttggtca tcttcatcat
    26881 cacgattgtc agtgctgagc cgatccgcaa ttttctggaa actcgctggg gactcagccg
    26941 tatcatcatt ttcttttttc tggtcggggc catctactgg ttgacgatgg cccgggttgt
    27001 tcgcggccag gtgatttcgc tcaagcatga gctgtacgtt gaagccgccc gggccctcgg
    27061 ggccagtttt tggcggattc tttttcgcca catcgtcccc aatttgataa gcatcatcgt
    27121 ggtctatttg acgctgacca tcccgcgggt gatgcttttc gaggcatttc tatcgttttt
    27181 gggcttaggg gtggaaccac cggatgtctc ttggggactg ctggctaacg agggacttca
    27241 ggccatgaca cctgtccgca tctactggtg gttgattgtc tttcccggcc tggctttggc
    27301 ggctacgctg tttgccttga acttccttgg tgatagcctt cgggatgcca ttgatccaag
    27361 attgagaaac gtcaggtgat gacgctcgtc acgatctctt tctggtgaag ccagcaaaca
    27421 ggaaggccgc ggtaaccgac gtctcaatcc acagaagggc gttccagacg ggccaagagc
    27481 ctccgagcca cctcggcagg gttagccgct tgaattacgc aaccactcac ggccacacgt
    27541 tgaattcccg tggccaatac ggacgcgagg ttttcctcgt tgatcccacc gatggcaaac
    27601 gccgggagac gaatctcttg ggcaacctgg cgaagaaaat ccagaccggc aaactccgag
    27661 aaactcttcg tggtggaggg gaatgtcgga ccgcagccaa tatagtcggc cccatccaaa
    27721 acggcttgtc gggcctgttc gatgttatgg gtggaaactc ccacaagggc acgggggccc
    27781 acgatgaaac gggcatcttt aactgttagt tcttcctgcc ccacatgaac cccgtcagcc
    27841 tcacttaaga cggccaagtc tgctcgatca ttcatgataa acagcgtgtt ggtcccacgg
    27901 gtcatctcgc gaatgaggcg ggcccgttcc aggagcaggc gatcgcagag atgtttctcc
    27961 cggagttgga tggcgtggac cccagcatcg atgagtgaac ctaccaggtt tcgcagctct
    28021 tccgccgagg acctggcatc caccagcaca taaaggcgga cacgctgcaa ccgttgtgcg
    28081 gcagtttcac tgatacgaag cgcccgatgg actgtataac agcgatatct caattgctcg
    28141 atcccggaaa cgatctgagg ggccaaaatc ttggcacact cctcaatgct ccgcaatgcc
    28201 tcttccaccc gcgagaaatt agcatcgaca aggtcctcca ccgccatgcg gtgaagctcg
    28261 gtggtcgctt tgatctccgt ccccacatcg tgttgggtct cacgggcggc cagacgaaca
    28321 ggcatgggaa aagccgtcgc caaaccagcc aattcgtgac gaatgttctt gatttcgcga
    28381 actagcaaag gatgatcgag ggcaaaacgc gcataatctt cgatcacgcg aaacccttcc
    28441 atggcccgat tgagcgccgc atcgaccatg cggaaaagcc gcatctcggt ggcgatccct
    28501 agtgagtccc tcgtcttttc ctcgcgattg tcaaagcgtt gaggcgtcga ttcagaagaa
    28561 ctcgttatct ccggtgcttg tcgtgacaac ggacttgccc tgtgagctgc ttcaggatca
    28621 aaaggcaaaa atgcttcccc ttccggctgg gcttcagaag aggaagataa ctccaggggg
    28681 acagtcggac tacgccattt cgtggccgtc ccatatcgtt ccaagatttg acggagtaga
    28741 tcgtgaaact ccaggcccat ttcgcgcaac caacaagcca cgggttcatc tccggccacg
    28801 agcccaagga ggaggtgctc ggttgagatg atcggcggcc aatctcccca atccatgtat
    28861 gtgtcaaccg cctcgagaat ggactccgcc tcggcgtgga aagcgggcgg tgattctggc
    28921 gttatcacgg gaggacgatc tgagcgcctc agttcgggcc agcggttgag gatggcagtt
    28981 tcctcgatac caatcgcccg aaggagtgca gcagcccggc attccggctc tgccaaaagc
    29041 cccatgagaa tggtgaccgg tcgtaagcca tcaaacgtct caccgagttg ccacgttgcc
    29101 gcagcggcca aagcccgcaa cgcaccggga gtgaattcac gttccatagt ggggttcaaa
    29161 ttctctgaaa taggaagaat ccaactcttc tgattagctt acaaaggcct ttggtagaga
    29221 gaaagcgatc ctccgaggca ccccataagg gcccgcgggg tttagtgaga ccgtttacag
    29281 gaaatggcct tgctgttaca tgtcggtggt cggtgttttc ggccgggtac ggaggaagcg
    29341 agcggcaagc ctcttgagcc gtccccaagg ggtggcactg gcttccggtg taacctcgct
    29401 caccattaag taggagtgaa agcttgtgat caggtcgtgc gcgcgaagga caatctcgcg
    29461 tcggcggcgt tcgcgtccac tcttcggcca aacctccaga tgaaggcgtg tccgggaggg
    29521 ctgtgtgact tcggttgaca cctcgctcag gtcaacgcga agtccaaagc aaatggggcg
    29581 gtcggccgaa cgtcgcgtcc gcagggggtt tcgatcctcg atctcgatct ccagatggtg
    29641 gcgatccaag tggacaacac ggcccaaatg gtcggcaatg aacccatgga gtttctccat
    29701 cgccagattg agtgggatag gactgataag atcctcacgc cagattaact cctgggagtt
    29761 gtggggccaa gaccaccaac gggaaaccgg tttttggatc gattccccat tgttcccggc
    29821 tcccaattgc acgaccacat tccggccacg ggatttcgcc atcagcaagg ctcggtcggc
    29881 tcgacggagg atggtctcag gggtatcgcc cggctggact tccgtcacac caaaactggc
    29941 agtgacaacg cggttgttga gtcggggctg gggagttcgt tccaacgcag cacgcatttc
    30001 ttcggctcgc cgtgtcgccg tggcaatgtc gcatccggcg cacagcatga cgaactcctc
    30061 gccgccatat cgggcaatga ggtcaccgtt ccgataaaaa ctgcggaaca cggcggcaag
    30121 gctacggatg gcatcatcgc cggcctggtg gccgtaggta tcattcacct gtttgaaacg
    30181 gtccaaatca caaatgatca agctaaatgt tagcccacgt cgctgatact gttccacgaa
    30241 caggggcaac attcggtcga attctgcgcg attggccaat tgggtcaggg gatccttaga
    30301 aacccgatcg cggaagtact cgagccgttg ttcgagagaa atctctgacg aggcatcgtg
    30361 caggatcgcc acggccccca agatgacacc atcgtcgaga atcaccggga cgacatgcac
    30421 atcgaccgat acaggtttgc ccgcacgtcc cttgatcgtc aaacgccgca ggacttgggt
    30481 cccgcagtta atggctgtga gaattgggca atccgattcg tcgatcggct gcccgcgctc
    30541 gttacgaaaa ccgaggatgg tcggtgacca ctgtcgcaaa cggacggcat cggccatgat
    30601 accactgagg cgttcggcgc catgattcca ttgcaggatg attttatgag tgtccacgaa
    30661 gataacgcca tcgcgcatcc actggagtag gcgatgctga aagacggtct ccaattcagg
    30721 tggggcgata caacgcgccg gcgacaccca tcgccatagt tggtttacag tggtacgatc
    30781 cagggcagaa agccagcgat gggcgaccgc ttcccgcgac gcttcgagtc cttcccgttg
    30841 gaaacgaaca aaatcctgga ccagatcggg atcgaactgc ttccctgcga aatgatagag
    30901 ctcggcaatc gcccgttctt cactcatggc ggggcgataa agatggtccg tcgtcatcgc
    30961 gtcatacgct tcgacgatgg caatcatgcg agcggcttcg gggattgccc gaccggacag
    31021 cacatatccc gaacgactgc cgtcgaacca tgccgaaacg ttttccacga ttcgtagtaa
    31081 gccttggtcg tcggtcacgt gcctgaggat ttccagcgtc atgaagcggg cgcgatcgac
    31141 ataaaccatt tcctcgggtg tgagagggcc aggcttcaag agaatctgat ctggcaggcc
    31201 aatcatcccc acatcatgta acagggcggc gagttcgaga cgatcttgtt cctctttctc
    31261 catcttgagg aaactggccc aggccgagga gaccagcgcg acccgcagcg aatggacagc
    31321 ggtgggggca tgacgcactt tgagcgcggt aaacagcccc gcggagaccc ccagccttga
    31381 agaaatcaac tgatcaaaag ctgaatccga agccggggca atgagctcct ctttcagagt
    31441 caagggggga atatcccttg ctgccgcggt cttgttttcg gacctcaaat gaccggtaga
    31501 gacgtcagga gtcattagcg acacgtttgg ggtcgcctgc gtttccgctg ccgatgatgt
    31561 cgatgccaag ttgctcatca gtggccccca atgtccccac ttcatctgcg gccagcctca
    31621 tgcacgaccg tcgccgaact tggtggtcgc ggatgaagat cagcaccgcc gcacacgaaa
    31681 tcacgtgcaa caagaggtta cgctttaacg accgacaaga atatccctga ctgacaagtc
    31741 tgtgaaacac accctattaa aggctaggtc agaatgggcc cggcgtcaaa tgagaagccc
    31801 gtaatccctt tttcatcccc atgggattgc agtgaccacg tttgctatat cgcttcccaa
    31861 aaacgtccgg aggcgggcaa agcctaccgg ccccactaaa gggtggaaat gactggaagc
    31921 cgcctttggt tccttagagc ctatcccata atcttccctc acccgttcgg cgggagaggg
    31981 gtcgggggtg agggtgactc aaaccgctgc tggttttctc aaacgattct gacgttcgct
    32041 tgccctcacc ccaaccctct cccggaggga gagggggact ttttggaata ggctcctagc
    32101 cgggaaaaag accaccctaa ccggtggtca agtggccgga cggacacaaa aaacaaaacg
    32161 cgcaccatgg cgtgcgggtc gccgagaaat cgaattgtta ccaaacttgg ttcacattct
    32221 ggccattcct tgtttggccg ccggtggtca acgggccttc ttgtaatcac ccacgttggg
    32281 aacgcgggga tggacatccg ggccgaagta gcggaggctc acgaggggtt ccgtaccggt
    32341 gttttccacg acgacaccgg ccttcgccgc ctcataggtg ataaacacct cgtcatccgg
    32401 ttcctcgcca aagcgaatca tgatcggtgt ttgcagccaa aggcttccga tcttgccgca
    32461 gccctgcaca gtgatccagc cgctggcacc cggatcgcgg aggacacact tggccccggg
    32521 catgaccgtc aactctttgg cggagaagag ttgtttgcca tccaccaagc catagacaat
    32581 ccagcggtcc gtatagccat cgccggaatt ctcttcatcc acgatgggtt ccaagtagtt
    32641 atgttccttg aagtacggat cgacattctt ttcccagtcg agctggctaa cgataaaatc
    32701 gagatcgtgg tgtttatctt tgggcacgtc tttgaccaag agatcccacg gaacataacg
    32761 cccttccacg atggattgga acat
//
LOCUS       DAOSUT010000023        32562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_940_length_32562_cov_9.793552, whole genome shotgun sequence.
ACCESSION   DAOSUT010000023 DAOSUT010000000
VERSION     DAOSUT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 32562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32562
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_32562_cov_9.793552"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1705)
                     /locus_tag="PLD05_03475"
     CDS             complement(152..1705)
                     /locus_tag="PLD05_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOP76522.1"
                     /translation="MNLRLISHILGVISLIVAGFMTASLPWSLPICGQVTHFDRRGFF
                     GVLAAILVSLIVSGLLLWYGRRARSDRLLRREALAAVGLAWLVATILGALPYICSGTC
                     RGIDDSGKPIRMTVFDAIFESASGYSGTGATVLADVENPDLVPRSVLFWRSETHFLGG
                     LGIVVLFVAILGMGSAGKQLIRAEVATPTQTSTHEQTRRAAMAFGTVFVVLNLILTFL
                     LMIQGLSIYDAICHAFGTVATGGFSTYNDSIGHFKSPLIDFTVVLFMLLGCTNFGLLY
                     FAAKGDIKRLVEDVEFRTYLAICMLATISVSICLAVQHPAFSSDMGRAIRCALFQCVS
                     IQTNTGFATDDFNEWPPYAKGILLMLMYVGGCAGSTSCSVKVIRYIFLFKICALEIEQ
                     AYHPNVVRIVRVGGRPVTDADLLKSVLVYFGLVVLTTAVAWLAILAVEPSSTWGVHPD
                     RKLLDCFSAVAATLNGVGPGLGLFGPLSNYGAMHAPAKCILTLLMLLGRLEYFPLLVL
                     VMPRFWKSA"
     gene            complement(1732..2844)
                     /gene="aroB"
                     /locus_tag="PLD05_03480"
     CDS             complement(1732..2844)
                     /gene="aroB"
                     /locus_tag="PLD05_03480"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOP76523.1"
                     /translation="MDESGVATVNVNLGERSYPIHIGNNLLAKRTSLLAGLDRVSHAV
                     IITDSNVGPLYLDQVSEIFQEAGVRTDSYVVPAGEKSKGLASAESIWNWMLDVKADRG
                     SFVVALGGGVVGDLAGFAAATFARGVRLLQIPTSLLAQVDSSVGGKVGVNLPKAKNMV
                     GAFYQPCAVWIDVSTLRTLPQREYLSGLAEVVKYGVILDSNFFSFLEEHVDDLLAQDP
                     AVLVSVVKTCCQLKATIVEQDEREVTGLRAVLNYGHTFAHAIETLTGYNQWLHGEAVA
                     IGMACAAKLAVRLGMFPEELAQRQESLLSSLHLPIRIRGLAADKILECMMRDKKVQHG
                     HLRFVLPTTIGRVVLVPDVSRQQVESVIDEMQDLSL"
     gene            complement(2864..3121)
                     /locus_tag="PLD05_03485"
     CDS             complement(2864..3121)
                     /locus_tag="PLD05_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76524.1"
                     /translation="MTMANELDPYREALVVEQVTIWPDDGYGLDRQNRQKVEAALHSH
                     PKSATELTYVRLPTGFCRRIRVTPEDITRVLGTGPLPSQHD"
     gene            complement(3322..4659)
                     /locus_tag="PLD05_03490"
     CDS             complement(3322..4659)
                     /locus_tag="PLD05_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76525.1"
                     /translation="MALRSSRREFLQASAVAGVGFWVAGGVRAEESKSPNEQIQFACI
                     GMGGKGQSDSADAGNHGVVVAICDVDDRTLEGASKRFPNAKKYFDFRKMLEEMGDKID
                     AVTVSTPDHTHAAAALMAMRMGKHCFCQKPLTRCIYEARLMGQVAREKKVQTQMGNQG
                     TANPALRRAAALLKKNILGTVKEVHVWTNRPIWPQGGERAAEQPVPPYLHWEEWIGPA
                     KFRPYAPGYHPFSWRGWWDFGTGALGDMACHTLNMPYAGLDLRNPVSVQAETSGHNRD
                     SFPKWSIITYEFAANDWRPALTLKWYDGGKLPPKELFTEFEDPKAPTASGALIIGEKG
                     KMYSPGDYCSEFKIVPKDLVVPEVEFERSPGHFEEWVAAIKTGKPAMSNFPDYASALT
                     ETVLLGNLAVWVANEPGVGPKVEWDAVNMKCTNISGLEEIVKPVYRPGYNLDA"
     gene            complement(5114..5575)
                     /locus_tag="PLD05_03495"
     CDS             complement(5114..5575)
                     /locus_tag="PLD05_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76526.1"
                     /translation="MCGSELQAKPKEKATGQERPGRILLVDDDRAAIEPLHIQLQIEG
                     FEVLLTETGQQGLDLARSWHPDVIVLDLCLPDVHGLEICQALMTTSATADIPIIILSG
                     MEGRDLPRRCRAAGGMYYVAKPYDPNVLLLLISQAAADHRLWHDAGSQNSE"
     gene            5852..6136
                     /locus_tag="PLD05_03500"
     CDS             5852..6136
                     /locus_tag="PLD05_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOP76527.1"
                     /translation="MIDPQLLSLLACPETRQPLSLGDSALLSQINQAIAVGKLRNRAN
                     CQVTQPLEAILVRQDGKVGYPVWDGIPTLLIDEGIPLGQLEMGGVENTSA"
     gene            6207..6551
                     /locus_tag="PLD05_03505"
     CDS             6207..6551
                     /locus_tag="PLD05_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOP76528.1"
                     /translation="MSPKTVFKKIIDREIPAEIVYEDQECLAFKDINPQAPIHVIVIP
                     KKEIPNLEELREEDEALIGHLFIAMQRIAQQMGISTGYRVVLNNGPDAGQEVMHMHFH
                     LLAGRKFDWPPG"
     gene            6565..>7572
                     /locus_tag="PLD05_03510"
     CDS             6565..>7572
                     /locus_tag="PLD05_03510"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOP76529.1"
                     /translation="MPLGLDGGVYIGIAYSMGYPDQDPLAWIEISLKELEDAGLRRQC
                     RTRECSQGRMIRFGERLLLNFGSNDYLGLAADTRLIQAVSEAIHREGWGSGASPLICG
                     RSISHAALESALARLEGTDAALVFPTGFAANSGTIAALVGPGDAVYSDKKNHASLLDG
                     CRLSRADFRVFPHADVDTLDRLLAKPHRHRRRLVVTDGLFSMDGDLAPLDRLVEVCRR
                     HSAMLLVDEAHATGIFGRLGRGVAEHFGVESEVQVRVGTLSKALGSVGGFVVGRRSLI
                     EWLINKGRPYMFSTAPPAAACCAVLTALNIVKTEPHRRETVLRQSDMLRKALRDDGWN
                     VG"
     assembly_gap    7573..7672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7673..10933
                     /locus_tag="PLD05_03515"
     CDS             <7673..10933
                     /locus_tag="PLD05_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HOP76530.1"
                     /translation="VLWQIGEVDKSPKGFALAPNQFAQYRGDAVFLVGVSSPEKDWPY
                     VQPGPLDVWAGSRTHQSTIIFSLEDAPLGPCELVLHLANTHGQHPPRMTVAVNNKVVH
                     EQVLPKGGPDPTITGDLSQAKPFEMRVPIAKEDLHSGTNTVTIASVAGSWIIYDAVVF
                     LAPPETRLSSPRDVTIVQRWESPPALIRHNGGLSQVVELTVLRGGEPTNASVSYEGKI
                     CWTGPIAPGENEICVPVPRVSKSTQGTLTVEVGNQTIWRQAVLLEPVREWEVYLLPHS
                     HVDIGYTELQTKVEQDHWRYYEEALEAWNATATFPPEARFKWNVEVLWAVDSYLRQAS
                     PERKEAFLQGVRDGAIGLQALYGNELTGLCRPEELLRLLDYAGRLEETYHFTIDSAMI
                     SDVPGYTWGIVTALAQAGVKYFSIGPNGGHRIGNTLRVWGDKAFWWKGPDGGSRVLCW
                     IPRRGYWRGFGGRDELMSYLKQLETEKYPFDMVQIRHCQGDNSGPDTGISKFVKEWNE
                     KYAFPKLIIATCSEMMRRFEARYGDVIPEVSGDFTPYWEDGAASSALETGLVRRAAEQ
                     LSMAEALWAMLRPSSYPDDRFYEAWRNVLLYDEHTWGAHNSITEPDSAFAQGQWAIKR
                     QFALDAARQAEELLTQSVASFGERNKSGRFLMVLNPCGWTRTDLVFVAPVSGHVAVLD
                     PEGRTVPSQRLATGELVFLAEDIPAFGGRRYELVALEVSDKGAPSPPVSGEEVLMSSS
                     RYELSIDASTGNIRRLFDKVSDRKIIASDGKYAGAEFLYVAGRDPKNVQRVSGGAKVN
                     WLDNGPLVQRVLVESAAPGCRNLRRTYEVVEKPDYVRIEFIIDKEKVRTPEGVHIAFP
                     FSIAGGEINLGLAWAVVNPHRDQLPGACKNYFTVQHWADVSNAEYGATWVSVEAPLVE
                     LGEIRMDLPNPFDPSTWLTELEPTQTLLSYVMNNYWETNYKADQEGPTPFVYYVRGHQ
                     GPCDPTAAARFGMERSRPLVAVFCQENAAKEIPSLISLNSPKVLPVECKPTRNGTGII
                     VRLFNFGDQPEEVTLGPGGQAKWKEIWRTDLSETKKEAIGGKILIKPRELVSLRLE"
     gene            complement(10985..11692)
                     /locus_tag="PLD05_03520"
     CDS             complement(10985..11692)
                     /locus_tag="PLD05_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76531.1"
                     /translation="MPLTVRCPSGHAFLAPPNFVGREVRCPYCQQTTVVDVAVSEHQT
                     SPAKPSSPGAAGGGEPSAPPNSAQGDPSSAEIINPVFNEVPSKVPTSRLSVGIVLGLL
                     AVAMSSLIPAALAIAQNCGGRWPTGVVAISALQIGAVFLLVILPDWTSLKIVGLLFGI
                     AAALFGMGAAFLAFAVESRLHAMGIESTLRDAAARWMISLMAIQATTTFLCFRAAETW
                     RRRVSIIFARKPRRATT"
     assembly_gap    11998..12097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12098..13075
                     /locus_tag="PLD05_03525"
     CDS             <12098..13075
                     /locus_tag="PLD05_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76532.1"
                     /translation="SRGKIPFWIGPSLQQIEPSGKVGEVLASFATAASIGFTWADGEP
                     APQKYCFDLADSQLMWCKQAGVLRGIGPLICLTPDRLPGWLSSDVPFSVIETAALNFV
                     AQTVRRFRAKVDFWEVLGPLNVTMPFRISEEKMALFAARVIQTARNLDPGVVIAISVR
                     QPWLEDLRSHECDYPAPFIVDALLRAGVGLDGIVLEMQLGYVPQGTFLRDRLDLARQI
                     DYWSKWGLPLFIRFCVPSRNDADPLCTSPFQTFPWPWSETEQSRWARDYVMMLVGCPL
                     VRGVFWSPWCDFEPHEFPHGGLFDLTQRPKEVTRFLAEFRKEWLAEPIR"
     gene            complement(13328..13780)
                     /locus_tag="PLD05_03530"
     CDS             complement(13328..13780)
                     /locus_tag="PLD05_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOP76533.1"
                     /translation="MNRRQAIVHMRQILLKRRDALRRALAGDLSLLRQLRTEAGGDIV
                     DFALDSVQDEISSQLAEIESRELARIEKALQRMDEGQYGVCEYCGCNIPMARLTALPY
                     ATLCVKCQREAELEGETVDRREVDWSRILDSNGSDNDITINDIELDVP"
     gene            14683..16629
                     /locus_tag="PLD05_03535"
     CDS             14683..16629
                     /locus_tag="PLD05_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76534.1"
                     /translation="MSTLCLETTSLVRNVQPERETEFVVKGSSRDGEVIRLKGRLITI
                     GSAPTCDLRLVAKGIRPLEGFLVRGRVNTVCRAYEGQFTLNGTPTSEGVIRPGDCLGV
                     GPVRLVFIPGGQRQTDAGSEGALPVGPEGVKERVSQMPRFPLRDDVSQLEANAVPDLP
                     TDKELPGDCAERACPVKPEADYLSANEVREIIESYLREAHLGGTANLAVVQAEVNDLR
                     QAMEETKDAVRKLQTELQSWIEKAAGWDEIAGRIAPTLDETVQETRRQQSVIEELVCQ
                     VRAELERLDRMEAEVVRRQIDLATERSRLEQTASALAFREQALADSGSVTSWTSESSQ
                     MKSPVGLGDDRLYLPGGPEPICDTDSGSSSRSEPAWPEDPIGRETIAYVSEPREPTDT
                     MPPEEGKVVDERLHCNNQAETPPKCDRAVLSLPESGRATTDSDPDHEVVIQDYLARLL
                     GKPVPRPPEKKDTFPKNADAGPPDGGAPGSGEKAEAGGKLPKLAANMPRQRAITRRRV
                     RAPEKNNNLAAMRELANLSSQAAIDRYAKAKLQRLQRGKLAVILVSSLCGLVLVLLDL
                     WVSIGQLGRSAMVLSFIVMVIYSIQYGILTGRLVINSKGQLQLAERRIGREMRKLLPS
                     NKPSLAEAGRDDEAQIEATPERVV"
     gene            17138..>18180
                     /gene="thrC"
                     /locus_tag="PLD05_03540"
     CDS             17138..>18180
                     /gene="thrC"
                     /locus_tag="PLD05_03540"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOP76535.1"
                     /translation="MTVGEGQTLLHPSDRVARYVGLNPGRLFLQYEGMNPSGSFKDNG
                     MAAAFTHARLIGATRAACASTGNTSASLALYCAVTGLMKAIIFIGSGKIAYGKLSQAL
                     DYGALTVQIAGDFDDAMKRVQEVSRSLGIYLVNSVNPFRLEGQKTIMFRVLEGLRWEV
                     PDWIAVPGGNLGNSSAFGKAFRELKELGLIPRVPRLAVINATGANTLYRLYEQQGVRW
                     NQGRVDKTQVGEFYQVMDQEQRRASTIASAIEINRPVNLMKCLQALETCQGVVREVTD
                     QEILDAKAQVGAGGLGCEPASAASVAGVKRLVAEGVIGRDERVVCVLTGHQLKDPTAT
                     VAYHTTDQEMFNKV"
     assembly_gap    18181..18280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18281..19009
                     /gene="dapB"
                     /locus_tag="PLD05_03545"
     CDS             <18281..19009
                     /gene="dapB"
                     /locus_tag="PLD05_03545"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOP76536.1"
                     /translation="LAMNEPDFEIVAAIDRADHPRLGQDAGVVAGVSPLGVSLKSSLE
                     TPADVMIDFSHPSATDKVIELCRLHRMALVFATTGISPEQEKALVELSRQVPIVRAPN
                     MSLAVNLAMKLCQIAAHSLREHDADVEIIERHHRFKEDSPSGTALQFGKTIAEIMGQT
                     IHRHGREGRPGRRPHEEIGYHAVRVGDNPGEHTIIFGMLGETLEITVRASNRDAYAKG
                     ALAAARFIVKQPAGLYGMQQVLQL"
     gene            complement(19048..20832)
                     /locus_tag="PLD05_03550"
     CDS             complement(19048..20832)
                     /locus_tag="PLD05_03550"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOP76537.1"
                     /translation="MYWTKTFIPTLKESPEGAEIPSHVLMLRAGLINQVMAGAYTYLP
                     LGIRSLRKAEAIVRQEMDAAGAIELMMPSMSPIGLWEQTGRVQAFGDVLVRAELKRHN
                     RKVTVVLGPTHEEIVTDLVAHHISSYRQLPITLYQITPKFRNEERPRFGVLRTSEFLM
                     KDAYSFDASVEGLNRSYEAMYRAYCRIFDRCCLSYIPVEAESGPIGGDASHEFMVPAD
                     NGEDKIVHCKMCGYAANLERAEIGSRKGDETSNRMQPLELVATPGASTIEQVSRFLQC
                     RPQDLIKTLIYLADGQPVAVLIRGDHEANENKIRRAFQAKEIVLADPKTIERVTGAPV
                     GFAGPIGLKENIPIIADYDIQYVRNGVTGANQADAHYRGVNLGRDFQVTRFADLRCAV
                     DNDPCPRCSGILRLRPAIEIGHVFKLGVKYSEALNARFLDADEQLKPIIMGCYGIGVN
                     RILAALIETTHDENGIVWPVALAPYEVVIVPVNVTDEQTRQVATALHDELSKEEIDVI
                     LDDRDVRAGVKFKDADLIGFPLRIVVGERGLAQNSVEVKWRWDKNSQMLPLDSVVREV
                     AQWIREERKTGQRFKSAVYGNRSGSPTR"
     gene            complement(21283..22749)
                     /locus_tag="PLD05_03555"
     CDS             complement(21283..22749)
                     /locus_tag="PLD05_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOP76538.1"
                     /translation="MADISAEVLAERAHDVGLLTDWEIRQIWSELGRKSVSLDEFIQF
                     LVRREYLTNYQAEKLLNGDRDGFFYRDYKVLYLVGAGTFARVFRAVHKDTKEVVALKV
                     LRRRYSDNPNCYQQFVREGRLGMALRHPNIVPVFDVCSYNGTHFLVMEFVEGQNLREF
                     VKVRKKLDPIVATRIMVDITSGLDYAFRQGLTHRDLKLSNVLISSNGTAKLVDFGLAA
                     VDEFLEAALPEIPANARAIDYAALEKATGVPRNDPRSDIYFLGCMYYHMLTGDPPLSE
                     TKDRMKRLSRNRFYEIIPIHQREPSTPKVVAGVINKAMSLDPETRYQTPGEMLIDLRI
                     AAKRLAQGEADVDLTAAKGGNADSNQPTVLVIEPNVEMQNVLREGLKKLGYRVLIISS
                     PQRALDRIRDETEGIDCIVIDAGELGESAVTAFNQIGDDPRTHMIPAVLMLEQHQRDL
                     AGMANRSDHRQVVTLPLTMKQLRGVVAGLVSKQRDFAK"
     gene            23083..23155
                     /locus_tag="PLD05_03560"
     tRNA            23083..23155
                     /locus_tag="PLD05_03560"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23116..23118,aa:Gly,seq:gcc)
     gene            23201..24733
                     /gene="tig"
                     /locus_tag="PLD05_03565"
     CDS             23201..24733
                     /gene="tig"
                     /locus_tag="PLD05_03565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOP76539.1"
                     /translation="MVASDGFRQDDEELKQASGLSEVEDADLMAPAATDESAGEAEES
                     TETPLEFEFDVEAAGPCKRKIIVTIPRKEVDRYFQEEFDKIRDEAQVPGFRPGRAPRK
                     LLEKRYRNELREPVKARLMLDAFTALADRANLAPISDPDLDPDRIELPDEGDFAFEFS
                     VEVRPEFEIPNWKGLKLTKTVYSYTPQYFEAYLREQLKSEALLEEVEEPAQLGDYITC
                     DISLFHEGRVLNSSDGERLLIRPRLTFMDGVIEDFGERMVGVQRGESRTCDVRVAESC
                     SDPELAGKTIQARFKVHAVLRRRSAENLEAFAQTMGYGSVEELRDAYERRLRSRLDQM
                     TYESLRKQILDQLLDVVPFDLPQDLVKRQTERELRRRAVELVRAGYSQEDIQPHLNAI
                     AREARTQVVRLLREHFLLERLAEAENIEADEDAIDAEVERIAMQTGRSPRQVRAELDQ
                     EEAWDVIRNAIVERRMIEKIIEVAEITEEQKTLTAREEAEETALDLGVVRPKPSAEET
                     SD"
     gene            complement(24810..24891)
                     /locus_tag="PLD05_03570"
     tRNA            complement(24810..24891)
                     /locus_tag="PLD05_03570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24855..24857),aa:Leu,seq:tag)
     gene            complement(25053..26054)
                     /locus_tag="PLD05_03575"
     CDS             complement(25053..26054)
                     /locus_tag="PLD05_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOP76540.1"
                     /translation="MTESVDHAATATPLHGVYQIIAEEMGEVDRLLRHELQDSHPYVD
                     RMLKHGLQLGGKRLRPALLLLAGKICGDINQTHIKLATAVELVHTASLIHDDVLDDAR
                     MRRHMATMNALWDNEASVLLGDYLLARAICLASSVDSVYACQVIAQASHEMCQGELRQ
                     IATRGKFDISEEEYIDIVAQKTASLYRACCRLGAYYAGATSRVVNDLEQFGTDLGIAF
                     QIIDDILDVLGDERRAGKSLGSDLDKQKPTLPIIRLLSVGNEDQRKELLTLLREQPEG
                     YRNRIRQMLAEQDAIPYAQDRAKGFVIAAMGRLSHFTPSPVLEAVRQLATFVISRDH"
     gene            26477..29464
                     /locus_tag="PLD05_03580"
     CDS             26477..29464
                     /locus_tag="PLD05_03580"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76541.1"
                     /translation="MSTWRPTRCFRWLVPAVLPVFLCTYMISGESCWGAVRIEKVITN
                     TCGEVRATSETKFGFYVFAGDDGAQLAVMPSAGTILAVLPARTHVTLSLRVSGSNEAE
                     KWELAVTSRVAGAAHVSVSFGQTTLSSRVTQDKPWTLPLPKGDPSELCEVTINPESDT
                     SRAVLLLELRNLGQLPQGDLIELTPGDPQVTPPPVCPNLHPEIQTAMIEWDWRQQDAL
                     DSCSPSLEAVHKAIARLIQQGDVLVDDLSQMGVQSDLFAQWRDVCGKWRNDDFQKDVA
                     SDVRAQLAVWQRIRTLKRQIALLNPYLAGKKLVFVKHVPSMFSHQLTQYTGNCARPGG
                     GLFVLEEPGRSMLARNLISGNFPLGSYQFCDVSPDGKKVLFSFCEVPSAPPDREAALD
                     RFFHLWEMDWDTRACRRLTDGSFDHFAGKYLADGRIVCVSTRRGGFHRCGRGPCPVHT
                     LTLLERDGSNARVISFHETHEWDPVQLLDGRILYTRWDYVDRNAVFYQQLWTVRSDGT
                     YVQAYYGNNTFNPVGIWEARPIPGTHAVMGTAAAHHAMTAGSIIRLDVCRGIDGPEPI
                     TRLTPDALFPESEVPVINRSGGRWFNPVGVTPPPLPEEQKRFPGHCYRTPFPLSEKYF
                     LVAYSYDPLIGEPDANQVNMFGIYLMDVFGNKELLYRDLNISSLWPVVVGNKPGTQPG
                     EAKVAVHLANHIIAGADEVASQSGRVSPTNESSPQAEGEFLLTNVYTAWPRLPPVKIT
                     RLRIVQVLPKSTWHANDPPLGLPNISPGKQVLGTVPVEPDGSAYFRAPAGKALSFQAL
                     DEMGQAVQIMRSVTYVQPGEIASCIGCHEPRNTAPAISHLPLAATRPPSEITPGPPGS
                     RPLSFPILVQPVLDRHCVRCHGENEPGGGIVLSGVPQGRYTKAYLALAPQVPYADWAG
                     RPGDFRVVNSEPVTQPDFFGARGSKLLSMLLEGHYDVHLDQDDLERLVTWMDCNALFY
                     GTFNPEDQKRQQKGEVILGPALE"
     gene            29926..31149
                     /locus_tag="PLD05_03585"
     CDS             29926..31149
                     /locus_tag="PLD05_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HOP76542.1"
                     /translation="MIRKVCDDGVGYSVVDLTDVRHVFAAAVPRCTGDLTTQTWDALR
                     TIQAVMKEESTLGSIVHQVVFLAEYDKVDECHEVMREFYGADFPATTYVPQKPCQGGI
                     VSIEALGVGRKRGDVVIDRRNEQLVIASHNNVSWVHCANIVPDPGIDGVYHQALNAFQ
                     KMNDMLGRAGVRFDQVIRTWLYLGGIVEGDGEGQRYKELNRARTDFYQGIPFLSDRLP
                     VHLKEAYPASTGIGADGHYLIMSCIALQTPRTDIVAMPLENPRQTPAFGYGCQYGIKS
                     PKFSRAMALSCGAYATIFISGTASIINSETQHIGDVERQTDETLDNIAALISEENLSR
                     HYLPQLGTTLDQLALVRVYIKRAEDYPKVRAICERRLGNVPTIYAVADVCRPELLVEI
                     EGIAFSQRGCQSGQC"
     gene            complement(31202..32122)
                     /locus_tag="PLD05_03590"
     CDS             complement(31202..32122)
                     /locus_tag="PLD05_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOP76543.1"
                     /translation="MEYRKMMRIAVTGSSGLIGSALVNHLRSLGHEVRRFVRREPQAA
                     DEISWDPERKLIDVRTIEGLDGIIHLAGASIAARRWSRSYKQLIRSSRIDSTQLLVEA
                     LQKTASPPRFFLCASAIGYFGDRGEEVLTEASPRGTGFLATVVEEWEATARYAEEYGV
                     RVVNLRFGQVLARHGGVVASLLPVFRLGLGGPIGQGLAWWSWILLEDVCRAVEFLLDH
                     AEIRGPVNITSPNPVRNRDFTKAFAKAIHRPAVLRVPTLLLKLLFGQLAQEVLLASAR
                     VIPVRLQESGFSFAYADIDKAFAKIFETVL"
     gene            complement(32204..>32562)
                     /locus_tag="PLD05_03595"
     CDS             complement(32204..>32562)
                     /locus_tag="PLD05_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOP76544.1"
                     /translation="ATGEVKRVMRVGQVIGLRPEKKDYYIQLHANTWPGVIKRIRDSN
                     IRNYSIYLAELEGKLYLFSYFEYVGDDFEADMAKIAADPETQRWWKETDPCQIRLPDT
                     PEGKWWKPIPEVFHTD"
BASE COUNT         7278 a         8589 c         9160 g         7235 t
ORIGIN      
        1 gccctcaccc taaccctctc ccagagggag agggggactt tgggataggc tctaagttgc
       61 gtacagagct tgtcacagtg gggcgcgcgg ctcagttcgg gctgcttcgg caatgtgggg
      121 gcgggcaact tggtatttgc gcgaatttca ttcacgcgct tttccaaaaa cgcggcatca
      181 cgaggaccag caggggaaag tattccaatc ggccgaggag catcaaaagg gttaagatgc
      241 atttggccgg tgcatgcatt gccccgtaat tggacagggg tccgaaaagg cccaggcccg
      301 ggccgacgcc attaagtgta gccgccacgg cggaaaaaca gtctaaaagt ttgcgatcgg
      361 gatgaacgcc ccaggtgctg gacggctcca cggcgaggat cgcaagccat gccacagccg
      421 tggtgagaac caccaggcca aaataaacga ggacactttt aagaagatcg gcgtcggtga
      481 ctggtcgtcc cccaacccgc acaatacgca ccacgttggg atgataggcc tgctcaattt
      541 ccagggcaca gattttgaag agaaagatgt agcgaataac cttcacactg caacttgtgc
      601 tcccggcaca cccgccaacg tacatcaaca ttaggagaat gcccttggcg taaggcggcc
      661 attcgttgaa gtcatctgtg gcaaaaccag tattcgtttg aatagagaca cattggaaaa
      721 gggcgcaacg gatagcacgg cccatgtctg aagaaaatgc cgggtgctgt accgccaggc
      781 aaatcgaaac cgaaatcgta gccagcatac aaatagctag atatgtccgg aactcgacgt
      841 cctccacaag ccgtttgatg tcaccttttg cggcaaagta caggaggcca aagttggtgc
      901 aacccagcag catgaagagc acaacggtga agtcaattag ggggctttta aagtgcccga
      961 tgctatcgtt gtaggtacta aatccgccgg tggctacggt gccaaacgca tgacagatcg
     1021 cgtcatagat ggataatccc tggatcatca aaaggaaggt caagatcagg ttgagcacga
     1081 caaacacagt cccgaaggcc atcgcggcac ggcgagtttg ttcgtgtgtg gaggtctgcg
     1141 taggcgtggc aacttccgct cgaatcaact gtttaccggc agagcccatg ccgaggatgg
     1201 ccacgaacag gacgacaatc cccaagccgc ccaaaaaatg tgtttccgat cgccagaaga
     1261 gcacggatcg gggaacgagg tcaggattct cgacatcggc gaggacggtt gcccctgtcc
     1321 cactgtagcc ggacgccgac tcgaagatcg cgtcgaagac ggtcatccgg atgggttttc
     1381 ccgaatcgtc gatacctcgg cacgttcccg aacagatgta tggtaaagcc cctaggattg
     1441 ttgccacgag ccacgctagt cccacggccg ccaaagcctc gcgccgcagg agtcggtcgc
     1501 ttcttgcacg acgaccgtac caaagaagca agccgctgac aatcagtgat acaaggatgg
     1561 ccgcaagaac gccaaaaaat cctcgtcggt cgaagtgtgt tacttgacca cagatgggga
     1621 gcgaccaggg caagctggcc gtcatgaatc ccgccacaat caatgaaata accccaagga
     1681 tgtgagaaat gaggcgaagg ttcataaccc ctatccagta tcggctgtgg atcaaagcga
     1741 caggtcttgc atttcgtcaa tgacggactc cacctgctgc cgggacacgt ccggaacaag
     1801 cacgacgcga ccaatcgttg taggcaggac gaaccgcagg tgcccatgct ggactttctt
     1861 gtcccgcatc atacattcca ggatcttgtc cgcggccaac ccgcgaatcc ggatcggcaa
     1921 gtgcaatgat gaaagaaggg attcctgtcg ttgagccagc tcctcgggaa acatccccag
     1981 ccggacggcc aattttgccg cacatgccat gcctatcgcc acagcctctc catgaagcca
     2041 ttgattgtac ccagtcaatg tttcgatggc gtgagcaaat gtatggccat aattgaggac
     2101 cgctcgcaac cccgttactt cgcgttcgtc ctgttccaca atcgtagcct tcagttggca
     2161 acatgttttc acaacggaaa cgagaacggc ggggtcctgt gccaacagat catccacgtg
     2221 ttcttcaaga aaagagaaaa aattggaatc aagaataaca ccatacttga ccacttcagc
     2281 caacccagag agatactcac gctgggggag cgttctcaac gttgagacat cgatccatac
     2341 tgcgcaaggt tgatagaaag caccgaccat gttcttggct ttgggaagat ttacgccaac
     2401 tttgccgcct acagagctat cgacttgcgc gagaagactg gtcgggatct gcaacagccg
     2461 gacacctctt gcaaacgtcg ccgccgcaaa gcctgccagg tcaccgacga cgcctccccc
     2521 gagggcaaca acgaaactac cccggtccgc cttcacatcg agcatccaat tccagataga
     2581 ttctgccgat gcgagaccct tgctcttttc gcccgcggga accacatagc tatctgtgcg
     2641 gacgcccgct tcttgaaata tctcagagac ctggtccagg tatagaggcc ccacattgga
     2701 atcggtaata atgaccgcgt ggctcacgcg atcgagcccg gctaataggg atgtccgttt
     2761 cgcgagcagg ttattgccga tgtgaatggg ataggaacgc tcgccgagat taacgttaac
     2821 tgtggcaacc ccgctctcat ccatggtgtt tctccggtgg tgtttaatca tgttgggaag
     2881 gcaaaggccc cgttcctaaa acgcgggtaa tgtcttcagg tgttactcgg atacgtcggc
     2941 aaaagcccgt gggcaaacgc acatatgtca attcggtagc cgacttcgga tggctgtgca
     3001 gcgccgcttc aaccttttgg cgattctggc gatccaagcc gtaaccgtcg tctggccaaa
     3061 tcgtgacctg ctcgacaacg agggcttcgc ggtacggatc caattcatta gccatcgtca
     3121 tcacactttt ccgacacgtg ttcaaagccc cacaaaccga gttaatatac ctcgtgcgtt
     3181 gcgtattaga aattctaatg gttatgaaga gaaaaaggga ccgaccagcc tgacggagga
     3241 cggtcccttc ggtgaccctc aaactcaaac gttgaccgtg gcatcggtgg cctggctttg
     3301 cgatgcctcg gtgttcatcg atcaggcatc gaggttatag cccggtcgat ataccggctt
     3361 gacgatctcc tcgagcccgg agatgttcgt gcacttcatg ttaacggcgt cccactcgac
     3421 cttcggtccg acgccgggct cattggctac ccatacggcc aggttgccca agaggacggt
     3481 ttcggtgagc gcgctggcat agtccgggaa gttcgacatg gccggcttgc ccgtcttaat
     3541 cgctgccacc cattcctcga agtgaccagg cgatcgttcg aattccactt cgggaacgac
     3601 caggtccttc ggcacaatct tgaactcgga gcagtaatcg cccggcgagt acatcttacc
     3661 cttctcaccg atgatcaggg caccgcttgc ggtcggggcc ttggggtctt cgaactcagt
     3721 gaacagctcc ttgggcggca acttgccacc gtcataccat ttcagggtca aagcgggacg
     3781 ccaatcgttg gccgcaaatt cgtaggtgat gatcgaccac ttggggaagc tatcccgatt
     3841 atggcccgag gtttcggcct ggaccgacac cggattgcgg agatccaaac cggcatacgg
     3901 catgttcagc gtgtggcagg ccatgtcgcc caacgcaccg gtgccgaagt cccaccaacc
     3961 gcgccacgag aacgggtgat aacccggagc atagggacgg aacttcgcgg gtccaatcca
     4021 ctcttcccag tggaggtaag gtggaacggg ctgttccgca gcgcgttccc caccttgcgg
     4081 ccaaatgggc cggttggtcc agacatgaac ctctttgacg gttcccagaa tgtttttctt
     4141 caagagggct gcggcccgcc gcaaagccgg attcgccgtc ccttggttgc ccatctgggt
     4201 ctgcaccttc ttttcacggg cgacttgacc catcaaacgg gcttcgtaga tgcaacgggt
     4261 caagggcttc tggcaaaagc agtgcttgcc catccgcatg gccatgaggg ccgctgccgc
     4321 gtgcgtatgg tctggcgtgc tgacggtcac cgcgtcgatc ttatcgccca tttcctcgag
     4381 catcttccga aagtcaaaat acttcttggc gttcggaaat cgcttggatg ctccctcaag
     4441 cgtccgatca tcgacgtcgc aaatggcaac gaccacccca tgattgccag cgtccgccga
     4501 atcactctgg cctttaccgc ccataccaat gcaggcaaac tgaatctgct cgttcggcga
     4561 cttgctctcc tcagcgcgga caccaccggc gacccagaac ccgaccccgg caacggctga
     4621 cgcttgcaag aactcacgac gacttgaacg caatgccata acgaatcctc ctcaagtaaa
     4681 cgtatgtttg tatggaccgg aagacctggt ctgccaagtc ttgaccaatt acgcgatagg
     4741 ataactcatt caaaaggctc ctgcaagttc cgcggaacca gggaggttaa atgacttcga
     4801 ggggacacct gcccgcgctg cgaatgtgaa cgccgaagaa ttacgagcgg cacctaacaa
     4861 tcactgataa ggcgatgata tcggtacgat gtccgcaaca tcttgacccc ccattgtagg
     4921 acggatgtgt ctcttgatca tatctctgaa tttttaaccc ctaggagaaa cgggcatcgc
     4981 tagtttgtac tggtggctcg tgtgattgag ggacgcagcg atgcgcgttt ctcaagggat
     5041 atggtgatgg aaggcgacga aacgagtgat taagggttac tcgccgtggc ggaggaactt
     5101 atgagaatgc aaatcactca gagttttgac tgcctgcgtc atgccacagt cggtggtcag
     5161 ccgcggcctg gctgatcagt aagagaagga cgttggggtc atatggcttc gccacatagt
     5221 acattccacc tgcggcgcgg caccggcggg gtaggtctct accttccata ccgctcagaa
     5281 taatgatggg gatgtcggcc gtagcagagg tcgtcattaa tgcctgacag atttctagac
     5341 catgcacgtc gggcagacaa aggtcgagga caatcacgtc ggggtgccac gaccgggcga
     5401 gatccagccc ttgctggccg gtctcggtta acaggacttc aaacccttca atctgcagtt
     5461 ggatatgaag cggctcaatt gcggctcgat catcatccac cagcagaatc cgaccaggtc
     5521 gttcctgccc ggttgccttt tctttcggtt tggcttgaag ttctgatccg cacatttgtg
     5581 gcgaaatttc cataaatacc ggcaagcaag ctacatctta gcattgagag cataggtcaa
     5641 gaaattggcc ttggcaaacg gaaaaaaaga aaatgcaaaa tagcgccctc gtgttctcag
     5701 gagataatgt ggtatatgat gaatattccg gaaggacgac ggttaatcgt agaacgttgc
     5761 cattttcttg ggctattgag acaatcgatg tccggttgtt cacgttgcgt cttttgacag
     5821 ctgtcaggaa cttcaagcca acgatcgaca gatgatagac ccacaattgc tttcactcct
     5881 ggcctgtcct gaaactaggc agcccctttc tttgggagat agcgctcttc ttagtcaaat
     5941 caatcaagcc attgcggtag gcaaattgcg taatcgtgcc aattgccagg tgacgcagcc
     6001 cttggaagcg attctggtta ggcaagatgg gaaagtgggt tatcctgtct gggatgggat
     6061 tccgacgttg ctcatcgacg aaggaattcc actcggtcag ctcgaaatgg gtggtgtcga
     6121 gaatacgtcc gcgtaagtgc ttcgaacagt ggttctggtg ttggcagttc agggaggctg
     6181 ggttttagac gcttggagag tgtgccatgt cgccgaaaac agtcttcaag aagatcatcg
     6241 atcgcgagat tccggcagaa atcgtttatg aggaccagga gtgtttggcc ttcaaagata
     6301 ttaatcccca ggcaccgatt catgtcattg tcatccctaa aaaggagatt cccaacttgg
     6361 aagaattaag ggaagaggat gaagcactga taggacacct cttcatcgcg atgcaacgaa
     6421 ttgcccaaca aatggggatc agcacggggt atcgggtcgt ccttaataat gggccagatg
     6481 ccgggcagga ggtgatgcac atgcattttc acctgctggc tgggcgcaag ttcgactggc
     6541 cacctgggtg attggtcttg cgggatgcca ttggggcttg acggtggggt ctatatcggt
     6601 attgcatatt ccatgggtta ccctgatcaa gacccactcg cttggattga aatcagcttg
     6661 aaagagcttg aagatgctgg tttgcgtcgg caatgtcgga cgcgtgaatg cagccaaggc
     6721 cgaatgattc gattcggcga acggctgctc ctcaattttg gatcgaacga ttacttgggg
     6781 ctagctgcag atacgcggct cattcaggcg gtctccgaag cgattcatcg ggaaggctgg
     6841 ggaagcgggg ccagtccgct gatttgtggc cgttctatta gccatgcggc cttagaatcc
     6901 gccttggcgc ggttggaggg gacggacgcg gctcttgttt tcccgacggg ctttgcggcc
     6961 aattcaggca caattgccgc cttggtaggg ccaggtgatg ccgtttacag cgacaagaaa
     7021 aaccacgcaa gtttgctcga tggttgccgt ctcagccggg cggatttccg cgtctttccc
     7081 cacgccgacg tggatacctt ggaccgcctt ctggcaaaac cccaccgtca ccgacgacgt
     7141 ctcgtagtga ccgatggcct cttcagtatg gatggtgacc tggcaccgct cgacaggctg
     7201 gtcgaagtgt gtcgccgtca ttcagcaatg cttctagttg atgaggcgca cgccacaggg
     7261 atctttggca ggctagggcg cggcgttgcg gaacacttcg gcgttgagtc cgaagttcaa
     7321 gtgcgtgtcg ggacacttag caaggcactg ggatcggtgg gcggatttgt ggtcggcaga
     7381 cgctctctga tcgaatggct catcaacaaa gggcgaccat atatgttttc aacggctccc
     7441 ccggccgccg cttgctgtgc ggtgctcacc gctctgaaca tcgtgaaaac agaaccccat
     7501 cgccgagaaa cagtgcttcg gcaatctgac atgctgcgca aagccctgcg ggacgacggg
     7561 tggaatgtgg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgctttg
     7681 gcagattggg gaagtggaca aatctccgaa gggtttcgcc ctggctccca atcaatttgc
     7741 ccaatatcga ggggatgcag tctttcttgt gggtgtttca tcgcctgaaa aagactggcc
     7801 ttacgtgcaa ccgggtccgc tggacgtttg ggcaggatca aggacgcatc aatcgaccat
     7861 cattttttcc ttggaagacg ctcccctggg cccgtgcgag ttggtacttc accttgcgaa
     7921 cacccacggt caacatccac cgcggatgac tgttgcggtc aataacaaag tggttcacga
     7981 acaggtacta cccaagggtg gccccgatcc gaccattacc ggagaccttt ctcaagcaaa
     8041 accgtttgag atgcgcgtgc cgatcgcaaa ggaagacctg cattcaggga cgaacacagt
     8101 tacgattgcg tcggtggcgg gtagttggat tatttatgac gcggtcgttt ttctcgctcc
     8161 tccagaaaca cgactgagct cgcctcgtga tgtcacgatt gtccaaaggt gggaatcacc
     8221 accggcgttg atccggcata atgggggcct ttctcaagtg gttgaattaa cggttctccg
     8281 tggcggagag ccaacgaatg cctctgtaag ctacgaaggc aagatctgtt ggacggggcc
     8341 aattgcgccg ggcgaaaacg agatttgcgt tcctgtcccg cgggtttcta aatcaacgca
     8401 gggtacgctc acagtcgaag ttggcaacca aacgatttgg cgtcaggcgg tccttttgga
     8461 gccggtgagg gaatgggagg tctatcttct tccacattcc catgttgata tcgggtacac
     8521 ggagcttcaa accaaggtgg agcaggatca ctggaggtat tacgaggaag ctcttgaggc
     8581 gtggaatgct acggccacct ttccgccgga ggctaggttc aaatggaatg tcgaggtcct
     8641 ttgggcggtt gatagctatc tgcggcaggc ctcgccggaa agaaaagagg cctttttaca
     8701 gggcgttcgc gacggtgcca ttggcctgca ggccctttac gggaatgaac tgacgggttt
     8761 gtgccgtccc gaggagcttt tgcgattact cgactacgcg gggagattgg aagagacgta
     8821 tcattttacc attgactcgg ccatgatcag cgacgtgccg ggttacacct ggggaattgt
     8881 gacggcgctg gcccaggcgg gcgtcaagta cttttcgatt ggcccaaacg gcggacaccg
     8941 catcggaaac acgctccgtg tttggggtga taaggctttt tggtggaaag ggccagacgg
     9001 ggggagccgt gtcctctgct ggattcctcg ccgtggttac tggcgaggtt ttggaggtag
     9061 ggacgaactg atgagttacc tgaaacaact ggaaaccgaa aaatatcctt tcgacatggt
     9121 ccaaatccgc cattgccagg gcgataactc agggccagac accggaatca gtaaattcgt
     9181 gaaagaatgg aacgaaaaat atgcatttcc caagctgatt attgccacat gttccgagat
     9241 gatgcgacgt tttgaagccc gatatgggga cgtcatccca gaggttagcg gtgattttac
     9301 gccctattgg gaggacgggg cagcgtcgtc ggctttggaa acaggtcttg tccgtcgtgc
     9361 tgccgagcag ctttcgatgg cggaggccct gtgggcaatg ctccgacctt cgagctatcc
     9421 tgacgatcga ttttatgagg cctggcggaa tgtacttctt tacgacgagc acacatgggg
     9481 tgcccacaat agcatcacgg aacccgacag tgcctttgcc cagggacaat gggcgataaa
     9541 acggcagttt gccctggatg ccgcacggca ggccgaagag ttgttgacgc aatcggtcgc
     9601 tagctttggc gagcgcaaca aaagcgggcg gttcctgatg gtccttaacc cgtgcggttg
     9661 gacgcggacg gaccttgtct ttgtagcacc agtgtcggga catgtggcgg tattagatcc
     9721 ggaaggaaga accgttccaa gtcagcgact tgccacaggg gaactggtgt tcttggcaga
     9781 ggatattccc gcttttggtg gccgacgata tgagcttgta gccctagagg tgagcgacaa
     9841 aggtgcccca tcgccccctg taagcggtga agaagtcctt atgagctcgt cgcgttacga
     9901 actcagtatc gatgcctcaa cgggaaacat cagacgtcta tttgacaaag tttccgatcg
     9961 gaaaatcata gcttctgacg gaaaatatgc gggcgccgag tttctgtatg ttgcggggcg
    10021 agatccaaag aacgtacaga gggtgtccgg tggtgccaag gttaactggt tagacaacgg
    10081 gcctttggtc caacgggtcc ttgtcgagtc agccgcgccg ggctgccgaa atctccgaag
    10141 aacctacgag gtcgtggaaa aacctgatta tgtccgcatc gagttcatca ttgataaaga
    10201 gaaggtgaga accccagagg gagttcacat cgcatttccc ttttccattg ccgggggtga
    10261 aatcaacctt ggattggctt gggcggtcgt caatccccat cgggaccaac ttcccggcgc
    10321 ttgcaagaac tactttaccg tccagcactg ggccgacgtc tcgaacgcag agtacggagc
    10381 aacctgggtt tccgtggagg caccgcttgt ggaactaggc gaaatccgca tggatctccc
    10441 caacccgttt gatccgagca cctggctgac cgaactggag cccacgcaaa cgctgctctc
    10501 ttacgtgatg aacaactact gggagacaaa ctacaaggcc gatcaagagg ggccgacgcc
    10561 ctttgtttac tatgtccggg ggcaccaagg cccttgtgac ccgaccgccg ccgcccgctt
    10621 tggcatggaa cgaagtcgcc ccctcgtggc cgtgttttgt caggaaaacg ctgcgaaaga
    10681 gataccttct ttaatctcac taaattcgcc caaggtgtta ccggttgagt gcaaacccac
    10741 ccgcaatggg acggggatta tcgtgcgtct cttcaatttt ggagaccaac cagaggaggt
    10801 tactcttggc cccggtggcc aggcgaagtg gaaggagatc tggcgaaccg atctgagcga
    10861 aaccaaaaaa gaggcaattg gtggaaagat tctgatcaaa cccagagagt tagtctcgtt
    10921 acgcctggag taaccaagcg acttgtgatt ggcaaggaaa ggacctgctg gtgtagcttg
    10981 gaagtcatgt ggtagcccgt cgtggttttc gcgcaaatat aatgctgacg cgacgtcgcc
    11041 aggtctctgc cgcgcggaag cagaggaacg ttgtggtggc ctggatggcc atgagggaga
    11101 tcatccatcg ggctgctgca tctcgtaggg tactttcgat gcccatcgca tgaagtcgcg
    11161 attccaccgc aaaggccaga aatgccgcgc ccatgccaaa gagggccgcc gcgatcccaa
    11221 aaagaagccc cacaattttc agagaagtcc agtccggcag gatcaccagg agaaagaccg
    11281 cgccaatttg aagggccgaa atcgccacta ctcccgttgg ccaacgtcct ccacagttct
    11341 gagcgatggc aagtgcggcc gggatcagac tgctcatggc cactgccaac agtcctagca
    11401 ctatgccgac cgacaaccga gaggtgggga cctttgaagg aacttcatta aaaacggggt
    11461 tgatgatctc ggctgagctt gggtcccctt gagctgaatt tggcggggcg gatggctcgc
    11521 caccaccggc agctcccggt gaagagggtt tcgcggggga ggtctggtgc tcactaaccg
    11581 cgacgtccac aaccgtcgtt tgctggcaat acgggcatcg aacttctcga cccacgaagt
    11641 tagggggtgc aaggaatgcg tgtcccgatg ggcagcgaac cgtcaacggc atggcagtct
    11701 catgggctag attaacacga gaggcccctg acatcgctca gtttaatcgg tgtggcgggg
    11761 gcttccaatg acgtggggtt aagaattcca ccagtctttg gccagcgaga cgcgtaaatc
    11821 gccttaggcg gtaccggaaa caccctttgc tagacaaatt gcccgggaaa taattgggga
    11881 tgcatggtac ccctaaaagt ggattcagcc gtcctgtgat gtgattgagg gaccatgttg
    11941 caaaaaggtt atcttgttta tgggaataat gcagtttcag gtttccgatg aggccccnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc cggggcaaga tcccgttttg
    12121 gatcggtcca agtttgcaac aaatcgaacc atcgggcaag gttggggagg tcttggccag
    12181 ttttgccact gccgcgagca tcggttttac ctgggcggat ggtgaaccag caccgcagaa
    12241 gtattgtttt gacctcgctg attcacaact catgtggtgt aagcaagccg gggtgttgcg
    12301 cggtattggt ccactgattt gtctcacccc ggaccgcctt cccggttggc tatcgtcgga
    12361 tgtcccgttt tcggtaatcg agacggcggc tctcaatttt gtggcccaga cggttcgacg
    12421 ctttcgggcc aaagtggatt tctgggaggt gctcgggcct ttgaatgtga caatgccttt
    12481 ccgcatctct gaagagaaga tggcactgtt tgccgcgcgt gtcattcaaa cggcacgcaa
    12541 cctcgaccca ggtgtggtca ttgccatcag cgttcgacaa ccgtggctgg aagatttgcg
    12601 atcgcatgag tgtgactatc ccgcaccttt cattgtggat gcactcctcc gcgccggcgt
    12661 tggtcttgac gggattgtcc tcgaaatgca gcttggctac gtgccacaag ggacgttcct
    12721 tcgggatcgt ttggatttgg ctcgccagat cgactactgg agcaaatggg ggcttcccct
    12781 gtttattcgg ttttgtgttc ccagccgaaa tgatgcagat ccgctttgca caagcccatt
    12841 tcagacgttt ccctggccat ggagtgaaac cgagcaaagc cgttgggccc gagattacgt
    12901 tatgatgctt gtgggttgtc ctctggtccg tggtgttttt tggtcaccgt ggtgtgattt
    12961 cgaaccgcac gagtttcccc acggtggttt gttcgacctg acccaaaggc ccaaagaggt
    13021 cacacgattt cttgccgagt ttcggaagga atggctggca gagcccatcc gctgatccaa
    13081 gttcgctctt ttgcatcaca aatgatagtc gcaaaagttg tggcggacgc catggttgga
    13141 aacacgtggg aaagaagacc acggtgaaaa tgacgcaaag cgagaaagcg caaaaaaagg
    13201 caaacgcctt gcgtttgcct tgaagatccg cccgaatgtt cctatcgcgt ctcaagcaac
    13261 catcgcgatc ctcggtgttc acgaccgtgt tagcatcgtg agccaccaag ggaacgatgg
    13321 cattagatca cgggacgtcc aattcaatat cgttgatcgt gatatcgttg tccgatccgt
    13381 tcgaatcgag gatgcgactc caatcgactt cccgccgatc gaccgtctcg ccttcgagtt
    13441 cggcttcccg ctggcatttc acgcacagcg tcgcataagg aagggccgtc agacgggcca
    13501 tcgggatatt gcaaccgcag tattcgcaaa ctccatattg cccttcgtcc atccgttgga
    13561 gggccttctc gatgcgtgcc agctcccggc tctcgatctc ggccagttga gagctaatct
    13621 cgtcctggac cgagtcaagc gcgaagtcaa cgatgtcgcc gccggcttcg gtacgcagct
    13681 ggcgcaagag actcagatcg ccggctagcg cccggcgaag agcgtcccgc ctcttaagga
    13741 gaatctgacg catgtggaca atagcttgtc gtcggttcat tggaattcca cctcagtgtt
    13801 tgctgtagcc caatgtcctg ttgatacgag tgtttccgct tttcagttca aaccgcccgc
    13861 cccccgcttc cacgcatact tcctgccaaa ctccccgctc cctactgggg cacctaccac
    13921 tatcttgacc ggttgatgcc tcccaagact tcggttcacc tctaagtcaa gttgcccaag
    13981 gaggcctttc ctcaccggta cattccgaaa tcaccacctc gggtcgcttt ttgcgccctt
    14041 acggaaatcc ggctatcttc ggcaaacgta gcgctaaggt tcaccaacgc aaatatagta
    14101 cgtaaacctt gcgggttggt ttcgtttagc cttttccgac tggtcgattt tcccttactt
    14161 gtacccggat tgagcaaatt tgcagcattt acaggtcgtt atgggctaag tcatgcaaaa
    14221 accgtgccga ctagcggcaa taatcaagga atcccgataa acacggtttt gctgtttatc
    14281 ccggttattc tgctgagcgt tatgggataa tcgacaaaac gaaacaatat atccaggaga
    14341 aatgaaaact taaacccgcc cctccgcacg aatttgacac cgccctgggt gcaacctaca
    14401 tctcattagg atttctgctg ggaagccgcc ctatctgcgg acaccaacct ccatgagacg
    14461 cagcgaacgg ccctccagtt tcaggaaatc cgcaatttcc tagcctagtg caccacactg
    14521 aataatggct ggccggaaca cgttgtgaca aggaaatggg agtaggtccc gttggtaatt
    14581 tcgctttccc ctcacaatcg cccgcagata atcctagtcg cgggcggcat tttgtaaata
    14641 gccgctaagg gttatccaat accacactta aggtcggtaa aaatgagtac actttgcttg
    14701 gaaaccacgt cgttggtgag gaatgttcag cccgagcgcg aaaccgagtt cgttgtgaaa
    14761 ggttcaagtc gcgatggaga agtcataagg ctgaaaggca ggctcatcac gatcggatcc
    14821 gccccaacct gcgacttgcg actcgtggct aaaggcatca ggcctttgga aggttttttg
    14881 gtcagagggc gcgtcaatac agtgtgtcga gcttatgaag ggcagttcac gctcaacggg
    14941 acacccactt cggagggggt tatccgcccc ggtgactgtc ttggagtcgg accggtgcgg
    15001 ctcgtcttta tcccgggagg gcaacgccaa acggatgccg ggtctgaagg tgccctgccg
    15061 gtcggtcccg agggagtaaa ggagcgggtt agccaaatgc caagatttcc tttacgagat
    15121 gatgtgtcgc aacttgaagc aaatgcggtg cccgatcttc ccaccgacaa ggaattgccg
    15181 ggagactgcg cggagcgtgc ttgccccgtc aagcctgaag cggattattt gagtgcaaat
    15241 gaggtgcgag aaatcattga gagctacttg agagaggccc atttaggcgg tactgccaat
    15301 ttggcagtcg ttcaggccga ggtgaacgac ctccgccagg cgatggagga gaccaaggac
    15361 gctgtgagga agcttcagac tgaattacag agctggattg aaaaggccgc cggttgggat
    15421 gaaatcgctg ggcgaatagc ccccaccttg gatgagacgg ttcaagagac gcgtcgccag
    15481 cagtcggtga tcgaggagct tgtttgtcaa gttcgggctg agcttgaacg actggatcgc
    15541 atggaagccg aggtcgtgcg gcggcaaatc gatctggcga ctgaacgttc gcggctggag
    15601 cagacggcct cggctttggc cttccgagaa caagcccttg ccgattcggg ctcggtaaca
    15661 tcgtggacaa gtgaatcttc ccaaatgaag tcgccggttg gtctgggtga cgatcggttg
    15721 tacctcccag gtggcccgga acctatttgc gacaccgact ctggcagcag tagcaggagt
    15781 gaaccggcat ggcctgaaga ccccatcggg agggagacaa tcgcttacgt atccgaaccg
    15841 cgagaaccga cggataccat gccaccggag gagggcaagg tagttgacga aagattgcat
    15901 tgcaacaacc aagcggagac accacccaag tgtgataggg cagttttaag cctgcccgaa
    15961 tctggaaggg ctacgacgga ttctgatccg gaccacgagg tcgtcattca agactatctc
    16021 gcccgccttt tggggaaacc tgtaccgcgg ccacctgaga agaaagatac cttcccgaaa
    16081 aatgccgacg cgggcccccc tgatggtggc gcacccggta gcggtgagaa ggcggaggcg
    16141 gggggaaagt tgccgaagct tgcggccaac atgccgcggc aacgggcgat cacaagacgc
    16201 cgtgttcgcg caccagagaa aaacaacaat cttgccgcga tgcgtgaatt ggcgaacctg
    16261 tcatcgcagg ccgcgattga tcgatacgcc aaagccaagc tgcaacgcct tcagcgcggg
    16321 aagctagcag taattctggt ttcatcgctc tgtggtctgg tcctcgttct gctcgatctc
    16381 tgggtgagca tcggacaatt gggccggtcc gcgatggttc tcagtttcat cgtcatggtg
    16441 atatattcga tccaatacgg cattctaaca ggccgtcttg tcattaattc caaggggcaa
    16501 cttcaactag ccgaacgtcg gatcgggcgg gagatgcgta aactgcttcc cagcaacaaa
    16561 ccttctctgg cggaggcggg ccgagacgac gaggcccaaa ttgaggcgac acccgaaaga
    16621 gtggtttagc ttcgggtaga ttggctaaaa gttttgtaag ccatcgggtg ggcgtctggc
    16681 gacgggttgg ttcaatactg cgcaaggagc acagaagcaa ctcagttgca caacgcaccg
    16741 cgggatatct tgcacgtttc cccgactgtg tgcgaaaatc cgcaagtaac gacatgtgcc
    16801 aagatgtgcg gttgtctgtt gctatgcaca ttctggatcg cgcttctttg cttttgctaa
    16861 gccagcggaa aggagcctag ccatcaatcc gacggattat gcctttcagc ggtgtatttt
    16921 cccagattgc ggggcaacgt ttggcgttga ggaaacccat atcggttgca cgcgatgtgg
    16981 cgggttgctt gaggtagcct acgaatggga taggctgcca gttccgtcct ctttacgcga
    17041 gtttgaacgg ctgtggggtc aaaggcacga tccgttcgct ttcagcggcg tgtggcgatt
    17101 ccacagtctt ttgccatttt cgccgagaga aaaatgcgtg accgtcggag agggccagac
    17161 gctcttacat ccctctgatc gagttgcccg atacgtggga ctcaaccccg gacggctgtt
    17221 tctgcagtat gaggggatga atccgtcggg gagtttcaag gacaacggaa tggctgccgc
    17281 atttacccat gccagattaa tcggggccac gcgggcagca tgtgcctcca ccgggaacac
    17341 cagcgcctct ttggcgcttt attgtgcggt caccggcctg atgaaagcca tcatctttat
    17401 tggttccggg aagattgcct acggaaagct ctcccaggca ctggattatg gggcactgac
    17461 ggttcaaatt gccggggatt ttgatgacgc gatgaagcgc gtccaggagg tcagccgttc
    17521 cttgggcatt tacctcgtta acagtgtgaa tccgtttcgg ttagaagggc aaaagacaat
    17581 catgttccgg gtcctggagg gactacgctg ggaggtcccg gactggattg ccgtccctgg
    17641 tggcaatttg ggtaatagta gtgcttttgg taaggcgttt cgggaactta aggaattggg
    17701 gttgatcccg cgcgtcccaa gattggccgt cattaacgcg acaggagcca acacgctgta
    17761 ccgcctttat gaacagcagg gtgtcaggtg gaaccagggg cgagtcgata agacccaggt
    17821 cggggagttc taccaggtaa tggaccagga gcagcggcgg gcgtcaacga ttgccagcgc
    17881 catcgaaatt aaccgaccgg ttaatctaat gaaatgtctc caggcccttg aaacctgcca
    17941 gggggtcgtc cgcgaggtga ccgatcaaga gattctggat gccaaggccc aggtgggtgc
    18001 aggtgggttg ggatgtgaac cggccagtgc ggccagcgtt gccggggtga agcggctcgt
    18061 cgccgagggg gtcattggtc gcgatgagcg ggttgtgtgc gtgctcacgg gacatcaact
    18121 gaaggatccc acggccaccg tcgcctatca taccaccgat caggagatgt tcaacaaggt
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggcgatga atgaaccgga
    18301 ttttgagatc gttgccgcta ttgaccgcgc ggatcatccg cgactggggc aagacgctgg
    18361 cgtggtggcc ggggtttctc cgttgggggt ttcgctgaaa agttcgctgg agacgcctgc
    18421 tgacgtgatg atcgatttct cgcatccgtc agccaccgat aaggtcatcg agttgtgccg
    18481 tcttcatcga atggcgctcg tctttgccac cacgggaatc agcccggagc aagaaaaggc
    18541 cttggtagag ttgagccgtc aggtgcccat cgttcgtgct cccaacatga gtcttgcggt
    18601 gaatcttgcg atgaagctct gtcagattgc ggcacatagt ttgcgcgagc atgacgctga
    18661 tgtggaaatc atcgagcgtc accatcgttt taaagaggat tcgccaagtg gtacggccct
    18721 gcaatttgga aagacgatcg ccgaaattat ggggcaaacg attcaccggc atggacgtga
    18781 gggccgaccc gggcggcgac cgcatgagga gatcggttat catgccgtgc gcgtgggaga
    18841 caaccccggg gaacacacaa tcatttttgg aatgttgggc gaaacgctgg agattacggt
    18901 ccgcgccagc aaccgtgatg cctacgccaa gggtgccctc gctgctgccc gcttcatagt
    18961 aaaacagcct gcgggcctgt atgggatgca gcaagtcttg caactatagt tgatcgcggt
    19021 tatctgcggc ggtacccgca agataattca ccgtgtcggt gagccacttc gattgccgta
    19081 gaccgcggac ttaaatcgtt ggccggtctt gcgctcttcg cgaatccact gagcaacttc
    19141 gcgaacgacg ctgtccaggg gaagcatttg gctgttttta tcccaccgcc acttgacttc
    19201 aacgctgttt tgagccaaac ctcgctcgcc aacaacaatc cgcagcggga aaccaataag
    19261 gtcggcgtcc ttaaacttca ccccagcccg aacatcgcga tcatccagga tgacatcaat
    19321 ttcctctttg gatagctcgt cgtgcagcgc ggttgcaacc tgtcgggtct gttcgtcggt
    19381 cacgttcacg gggacaatga cgacctcgta gggggccaac gccaccggcc agacgatgcc
    19441 attctcgtcg tgggtggtct cgatcaaggc cgcaagaatc cgattcactc cgatcccgta
    19501 gcagcccatg ataattggtt tgagttgttc gtccgcatcg agaaaccggg cgttaagggc
    19561 ctcgctgtat ttcacgccca acttgaagac gtggcctatc tcgatggcag gccgcagtcg
    19621 cagaatgcca ctacaccgcg gacacggatc gttatccacg gcgcatcgta aatccgcgaa
    19681 ccgggtcact tgaaagtccc gccctagatt gaccccacgg tagtgagcgt cggcctggtt
    19741 ggccccggtt acgccgttgc ggacatattg aatgtcgtaa tcggcaataa tcgggatatt
    19801 ctctttcagg ccgattggcc cggcaaagcc caccggggcc cctgtaacgc gttcgatggt
    19861 tttgggatcc gccaggacga tttctttggc ctgaaaagcc cggcggatct tgttttcgtt
    19921 ggcttcgtga tcgccccgaa tgagtacggc gaccggttgc ccgtcggcga ggtaaatcaa
    19981 tgtcttgatg aggtcctggg gccgacattg caagaagcga cttacctgct caattgtgct
    20041 tgccccaggg gttgccacca gctcgagtgg ttgcatgcgg ttggacgttt catccccctt
    20101 gcgggaaccg atctcggccc tctccaggtt cgccgcgtaa ccacacattt tgcagtgaac
    20161 gatcttatcc tcgccgttat ccgcgggcac catgaactcg tggcttgcgt caccgccgat
    20221 cggaccactt tccgcttcca ctgggatgta agacaaacag caccggtcga aaatccggca
    20281 atacgcgcga tacatcgcct cgtaacttcg gttcagtcct tccaccgaag cgtcgaagct
    20341 gtaagcgtct ttcatcaaga actcgctcgt tcgcaacacg ccaaaacgtg gtcgctcttc
    20401 attgcgaaac ttcggtgtga tttgatagag cgtgataggt aattgacggt agctggaaat
    20461 atgatgggct accaagtcgg tgacgatctc ctcgtgtgta ggaccgagga cgacggtaac
    20521 cttccggtta tgacgtttca attccgctcg aactaaaaca tcgccgaagg cctgaactcg
    20581 ccccgtttgc tcccaaaggc cgattggact catgctgggc atcatgagct cgatggcgcc
    20641 cgccgcatcc atctcttggc ggacaatcgc ctccgccttc cggagggaac gaatccccag
    20701 aggcaggtac gtgtaggccc ccgccataac ttggttgatc aatccggcac gaagcatcag
    20761 aacatgacta gggatttcag ctccttcagg cgactctttc aacgttggga taaaggtctt
    20821 tgtccaatac attgcgtttt acctcgatcg ttccgtgcca tgtcgtcaca acgtccggct
    20881 ccgcgtcgaa attgctgatc ttcgacacgt tgccgggctc gtatttgggc ttgttctcgc
    20941 cataagcccc gaaaacgacg attctagacc gcagagcgta aattggacaa gatcggccga
    21001 atggaagaaa cttctcgatt gtgcgaaagg cagtacttct cggaaatcgc tcgcggaggg
    21061 gatccgacca tcgtcgggaa ggctttttat gcaagaccat ctagatggaa aagcgactca
    21121 acggggctgc cttacgcctt ctcttgtcct tccggcgcag acccgttgtc ttttgatccc
    21181 aaagcaaacg acgccggctt ttccctctgg tacggccccc ctcatgacga aagtgcactt
    21241 tcttggggat cagatgcccg tttcgtgcga caaagacaga gattatttgg caaaatcgcg
    21301 ctgttttgat accaacccag caacgacacc gcggagttgt ttcatcgtca gcggcaacgt
    21361 tacgacttgg cggtgatcag accggttggc cattcccgcc aagtcacgct ggtgctgctc
    21421 aagcatcagg actgccggaa tcatgtgcgt gcggggatca tcgccaattt ggttgaaagc
    21481 ggttactgct gattcaccca attcaccggc atcaatgacg atgcagtcaa tcccctcggt
    21541 ctcatccctg atccggtcga gtgctcgctg gggtgacgaa atgatcagga ctcggtagcc
    21601 aagtttctta agcccttcgc gcaagacatt ttgcatctcg acattaggtt caatgaccaa
    21661 aactgtgggt tggttcgaat cggcgttgcc ccccttggct gcggtcaggt caacatccgc
    21721 ctcaccttgg gcgagccgtt tggcggcgat gcggaggtcg atgagcattt ctccgggggt
    21781 ttgatatcgg gtctcgggat caagagacat cgccttgttg atgacacccg ccacgacttt
    21841 cggtgtcgag ggctctcttt gatggatcgg aattatctca tagaaacggt tccggcttaa
    21901 ccgtttcatg cggtcctttg tttcggaaag cggtgggtcg cccgtaagca tgtgatagta
    21961 catacagcct aagaaataga tatcgctccg cggatcgttg cgggggacgc ctgtcgcctt
    22021 ctcaagtgcc gcatagtcaa tggcccgcgc gttggcgggg atttcgggca aagcagcttc
    22081 taggaattca tcgactgcag ccagtccgaa atcgaccagc ttggccgttc cattgctgct
    22141 gataaggacg ttgctgagtt tcaagtcacg gtgggtcaac ccttgacgaa aggcgtagtc
    22201 cagcccggat gtgatatcta ccatgatccg ggtggcgacg atggggtcca atttcttgcg
    22261 gactttaacg aactcgcgaa ggttttgccc ctccacaaat tccatgacaa ggaagtgcgt
    22321 cccgttgtac gaacagacgt cgaacacggg aacgatattg ggatgccgaa gggccatccc
    22381 caagcgacct tccctcacga attgctggta acaattgggg ttgtcactgt agcgccgcct
    22441 caagaccttg agggccacaa cctccttggt gtccttgtga actgctcgga aaacacgggc
    22501 gaatgtaccc gctccgacca gataaagaac cttataatcg cgataaaaga aaccgtcacg
    22561 atcaccgttt aggagttttt cggcctgata gttggtgagg tattcacgcc gcaccaagaa
    22621 ttgaatgaac tcatccagcg agacgctctt tctgcccaac tctgaccaaa tctggcggat
    22681 ctcccaatcg gtgaggagac caacatcatg ggctcgctcc gccagcactt ctgctgaaat
    22741 gtcggccata atctctccct ctgttccgag tattggctca attatgggcg agttaacccg
    22801 ctctgatcag cggcacaaaa tctgggtaaa tgccccttcc aatgaaatac cccgagcacc
    22861 caccgtcccc ctaaaagcgg taaaccggta tctatcatgc tttatcttac tttgcacccg
    22921 ccgagatggc aacgagattg gaaccctctg aaagtggggt ttcgatggaa aatgtcggct
    22981 ctgcccctat gcaagagcgg tggagtttcg ccccccttgc gtgaacgttt cttagccgta
    23041 taatgccaac taagagagag gcgataacga aaatcgatcg atgcgggtgt agctcaggtg
    23101 gtagagcacg acgttgccaa cgtcgttgtc gtgggttcga gtcccatcac ccgctttttt
    23161 actttgtagg tttgggtgat ttagatggtc aggggtttcc atggttgcct cggatggttt
    23221 ccgtcaagac gacgaagaat tgaagcaggc ttccggcctg agcgaagtcg aagatgcgga
    23281 tttgatggcg cctgccgcca cagatgaatc ggcgggcgaa gcggaagaaa gcacggagac
    23341 accactcgag ttcgagttcg atgtggaggc tgccggtccc tgcaagcgga aaatcatcgt
    23401 gacgattccg cggaaggagg tcgatcgcta tttccaggaa gagttcgata agattcggga
    23461 cgaggcacaa gttcctggtt tccgtcccgg tcgggcacct cggaaactct tggaaaagcg
    23521 ctatcgtaat gagctgcgcg agccggttaa agcgcgtttg atgctggacg cctttaccgc
    23581 ccttgcggat cgggcaaatc ttgccccaat tagcgatccc gacttggacc ccgaccggat
    23641 tgaattgccg gacgaaggtg attttgcatt tgagttttcg gtggaggtac ggccggagtt
    23701 tgaaatcccc aattggaagg gcctcaagct caccaagacg gtttactcct acacacctca
    23761 gtactttgaa gcgtacttgc gcgagcagct taagagtgag gccttgctcg aagaggtgga
    23821 agagccggcc caactgggcg attatatcac ctgtgacata tccctctttc acgaaggtcg
    23881 cgtcttaaac tcctccgatg gtgagcgatt gttgatccgg cctcgtttga cattcatgga
    23941 tggtgtgatc gaggatttcg gggagaggat ggtcggcgtc caacggggcg agtcgcgtac
    24001 ctgtgacgtg cgggtggcgg agtcttgttc tgatcctgag cttgccggca agacaattca
    24061 agcccggttt aaagtccacg cggtactgcg acggcggagt gctgagaatc tagaagcgtt
    24121 tgcccagacg atggggtatg gctccgtcga ggagttgcgg gatgcttacg agcggcgtct
    24181 ccgctctcgc ttagatcaaa tgacgtacga gagtctgcgt aagcagattc tcgatcaact
    24241 tttggatgtg gttccatttg atttgcccca ggatctcgtc aagcgccaga cggagcgaga
    24301 gcttcgcagg cgggctgtgg agctggttcg ggccggttat tctcaagagg atatccaacc
    24361 gcatctcaat gcgatcgcgc gggaggccag gacccaagtc gttcgtctgt tgagagaaca
    24421 tttcctgctt gaacggttgg ctgaagcgga gaatatcgaa gccgacgagg acgctattga
    24481 cgcggaagtg gagcgaatcg cgatgcaaac cggtcgctct ccacggcagg tccgcgcgga
    24541 gctggatcaa gaagaggctt gggatgtcat ccgtaatgcc attgtcgagc ggaggatgat
    24601 tgaaaaaatt atcgaggtag ccgagatcac tgaggagcag aagacgttga ccgctcgcga
    24661 ggaagccgaa gaaacggccc tcgacctggg agtagttcga ccgaagccaa gtgccgagga
    24721 aacatcagac tgagacaaaa gatgcgtcca agccaaactc gtgagaccgc ccttagtggc
    24781 ggtctttttt attcagtggc ttgaaagaat gcggacggag ggactcgaac cctcacccct
    24841 tgcgggacaa gatcctaagt cttgcgtgtc tgccaattcc accacgtccg cacaactaag
    24901 acgttggata ggcctaaccg tcaacttttc tgtcgaccct gctttgattc tactaagttc
    24961 ccgcgaccgt tgccagatcg gtcgaaggca ccagaaagcc gcgcaattac gcgttgcgtg
    25021 aacacgccgt cgagcagaaa cggcttccaa gatcagtggt ctcgtgagat gacaaaggtc
    25081 gcgagttgtc ggacggcctc gagaactgga ctgggggtga aatgagacag tcgccccatt
    25141 gcagcaatca cgaaaccttt cgcacgatct tgggcgtagg gaattgcgtc ttgttctgcg
    25201 agcatttggc gaatacggtt tcggtagcct tccggctgct cacggagcaa tgtcaacaac
    25261 tcttttcgct ggtcttcatt acccacggac aacagtcgaa tgatgggcag cgttggcttc
    25321 tgtttgtcca gatcactacc caaggatttt ccagcccgcc gttcatcacc tagcacgtca
    25381 aggatatcgt cgatgatttg aaaggcgatt cctaagtcgg taccgaactg ctcaaggtcg
    25441 tttaccacac gggaggtcgc accggcataa taggcgccga ggcgacagca tgcccgatac
    25501 aacgaggccg tcttttgtgc aacgatgtca atgtactcct cttcggagat gtcgaacttc
    25561 ccccgggtgg caatttgtcg caattctccc tgacacatct cgtgactggc ctgggcaata
    25621 acctgacagg cataaactga atccacggaa ctggccaggc aaatggccct tgccaggagg
    25681 tagtcgccca acaggacgct ggcctcgttg tcccagaggg cattcatggt tgccatgtgc
    25741 cgacgcatcc gggcatcgtc caaaacgtca tcatggatga gactggccgt gtggacgagc
    25801 tcgacggctg ttgccagttt gatgtgcgtc tgattgatat caccgcagat ttttcccgca
    25861 agcagaagaa gagccggccg caggcgtttg ccgcccaact gcaagccgtg cttgagcatt
    25921 cgatcgacgt agggatgaga atcttgtaat tcgtgacgca acaagcgatc gacttcgccc
    25981 atttcctcag cgatgatctg gtaaacgcca tgtaaaggcg tggctgtggc ggcatgatcg
    26041 acgctttctg tcattcggcc atcctttaga aacacctaga ttgattctct tctgtcgcta
    26101 tctgattttt tataggcgca agtcttcgtg gcgtgccggt tcttccggtt ttacaggcat
    26161 gccacaatcc cactcaattt gtttcatcga cacatttagt gtacttgcaa cgattaagcg
    26221 ctggcaatcg cttatgggac cgttttgttc gacggcaagc gggggtcgca agaaccggct
    26281 tgagccaagc ggcgtcccaa actcactact tctctcttct ttactactgt cagactgtca
    26341 aaactgtttg atcttcgcca ccagaagtgg tagcatcttg gttaacggcc agcagtgggc
    26401 ggcttccaaa gaattggaag cccaactttg cgggggccgg cggtattggc tttacccatg
    26461 tgaggagcgg aaaccaatga gcacgtggcg gcctacgcgg tgttttaggt ggcttgtgcc
    26521 cgctgtccta ccggtgttcc tctgcaccta catgatttcg ggcgaatcct gttggggcgc
    26581 cgttcgaatt gaaaaggtca tcacaaacac atgcggcgaa gtgcgggcga ccagcgaaac
    26641 gaaattcgga ttctacgtct ttgccgggga cgatggggcc cagttggccg tgatgccgtc
    26701 ggcggggacg attcttgccg tccttccagc acgaactcat gtcacattgt cgcttcgtgt
    26761 ctcgggttcg aatgaggcgg aaaagtggga attggctgtg acttcccgcg ttgccggggc
    26821 tgcccacgtt tccgtgtctt tcgggcaaac gactctttct tcgcgggtca cccaggataa
    26881 accgtggacg ctgccgctgc cgaaaggtga tccttcagaa ctgtgtgagg tgacgataaa
    26941 ccccgaaagc gatacgagca gagcggtgct tcttttggaa ctacgaaacc ttgggcagtt
    27001 accacaaggc gacctcattg agctgacgcc cggtgatccg caagtgactc ccccgcctgt
    27061 gtgtcctaat ttgcatccgg aaatccaaac cgccatgatc gagtgggact ggcggcagca
    27121 ggacgctctg gattcatgtt ccccatcgtt agaggcggtt cataaagcca ttgcgagact
    27181 catccagcag ggtgatgttt tggtagatga tctctcgcaa atgggggtgc aatcggatct
    27241 atttgcccag tggcgggacg tgtgcggaaa gtggcgaaac gacgatttcc agaaggacgt
    27301 ggcatcggac gttcgtgccc aactggccgt gtggcagcgc atacggacgt tgaaacggca
    27361 aattgcgctt ctaaatcctt atcttgctgg gaagaaactg gtgtttgtga agcatgtccc
    27421 cagcatgttc agtcaccagt tgacgcagta cacaggcaat tgcgcgcggc ccggtggggg
    27481 gctttttgtg ttggaagaac caggtcggtc gatgttagcc cgtaacctaa tttcgggtaa
    27541 ttttccgctg ggaagttacc agttctgtga cgtttcaccc gacggcaaaa aggtgctttt
    27601 ttctttctgt gaggtgccat ctgccccacc cgaccgtgag gcggcccttg atcgcttttt
    27661 ccatttgtgg gagatggact gggacactag ggcctgccga cgtttaaccg atggatcctt
    27721 cgaccatttt gctggcaagt atctggccga cggtcggatc gtttgcgttt cgacccgtcg
    27781 gggtggtttc catcggtgtg gtcgagggcc ttgtcctgtt cacacattaa cgcttttgga
    27841 aagggatggc tccaacgcga gggtgatttc cttccacgag actcacgaat gggacccggt
    27901 tcagcttttg gatggccgga ttctttacac acggtgggat tatgtagata ggaacgccgt
    27961 cttttaccag cagctttgga ctgttcggag tgatggcaca tatgttcagg cttattacgg
    28021 taacaacaca ttcaaccccg tcggtatttg ggaggcccga ccgataccgg gcacacatgc
    28081 ggtgatgggc acggccgccg cccatcatgc catgaccgcg ggatcgatca ttcgcctcga
    28141 cgtatgccgg ggaattgatg ggcccgagcc aataactcgc ctaacaccgg atgccctttt
    28201 tccggagagt gaagtccccg tgatcaatcg gtcgggcggg cggtggttca acccggttgg
    28261 ggtgacgccc cctcccttgc ccgaagaaca gaagcgattt ccgggtcatt gctaccgaac
    28321 gccatttccc ctgtcggaaa aatacttcct tgttgcctac agttatgacc cgttgattgg
    28381 cgaaccggat gccaatcagg tcaacatgtt cggcatctat ctgatggatg tttttggtaa
    28441 taaggaattg ctgtaccgcg acttaaacat ctcgagtctc tggcccgtcg ttgtcggaaa
    28501 caagcccggt acgcaaccgg gtgaggctaa agtggcagtt catttagcga atcacattat
    28561 cgcgggtgca gacgaggtgg catcacagag tggacgagtc tctccaacga atgagagctc
    28621 acctcaagcg gagggagagt ttctattgac caatgtttac acggcatggc cacgactacc
    28681 accggtcaag atcacacgac tgcggattgt ccaggtgctg ccgaaatcga cgtggcacgc
    28741 caacgatccg ccgttgggac tgcccaatat ctctccgggg aagcaggtct tgggtacggt
    28801 ccccgtggag ccggacggct ccgcatattt tcgggcccca gcgggaaagg ctttgtcctt
    28861 tcaagccctc gacgagatgg gccaggcggt tcagatcatg cggagtgtta cttacgtcca
    28921 accaggcgaa atcgcttctt gcatcggttg ccatgagccg cggaataccg cacctgccat
    28981 cagccatctt ccgcttgcgg caacacggcc accgtcagag atcacacccg ggcccccggg
    29041 ctcgcgaccg ttgagctttc cgatcctcgt tcagccggtg ctcgaccggc attgtgttcg
    29101 atgtcacgga gaaaacgaac cgggtggcgg catcgtcctc agtggggttc cacaagggcg
    29161 ttatacaaag gcctatctcg cgcttgctcc ccaggtgccc tatgccgact gggcaggccg
    29221 tcccggcgat ttccgggttg tcaactctga acctgtcacg caacccgatt tctttggtgc
    29281 tcgaggatca aagctcctga gcatgctctt agaagggcac tatgatgtcc atctagatca
    29341 agatgacctg gaaaggctgg taacctggat ggactgcaac gccctgtttt atgggacatt
    29401 caatcctgag gaccaaaaac ggcagcagaa aggggaagtc attttgggac cggcattgga
    29461 gtagtgccag tgcgctgtga agggaaagcg ctattctacc tttggaggga gtgggcaaaa
    29521 aacggagaag attgttgcaa atgcgatatg atgcggttag actgtaaaca acatcgtagt
    29581 ttttgccttc atgctgaccc ccgcatttgc gacacaagaa tcggaacaaa ttcctacctt
    29641 ggattacctc ggccatttgt tcagggaagt tgtcattttt tgcaacgcaa cagtgtaaat
    29701 aatagtggcc gcttggtgtt ttgatgccgc accgagatta caactttcac gccgcgggct
    29761 gcggacggat cgtgcgtcag tgacgaatcg ccacctgttg ctttgtgaac actgtgttaa
    29821 caaagggcga attcccgcaa cgttcgggcg taaaacctgg tgcgaaccca ataaccttta
    29881 gcagaaggca ttctcacggt gaccctccaa acgcgagaac gcagcatgat tcggaaagtc
    29941 tgtgatgatg gggtcggcta ttcggttgtc gatctcacgg atgtccgaca cgtgtttgcc
    30001 gcggctgtgc ctcgttgcac cggggacctc acaacgcaaa cgtgggatgc ccttcgcacc
    30061 attcaggcgg taatgaaaga agagagcaca ttaggctcca tcgtccatca ggtggtcttc
    30121 cttgccgaat acgataaggt tgacgaatgc cacgaagtaa tgcgggagtt ttacggggcg
    30181 gacttcccgg caacgacgta tgtgccgcag aagccctgcc aaggggggat tgtttccatc
    30241 gaagcactag gcgtgggacg gaaacgtggc gatgtcgtca ttgaccgacg aaatgagcag
    30301 cttgtgatcg cgtcgcataa caacgtctca tgggtgcact gcgcgaacat tgtgcccgat
    30361 cctggaattg acggggtcta tcatcaagcc ttgaacgcct tccagaagat gaacgacatg
    30421 ttgggaaggg ccggggtgcg attcgatcaa gtcattcgca cgtggctata tctgggtggg
    30481 attgtcgaag gagacggcga aggacagcgt tacaaggaac tcaatagggc ccgcacggac
    30541 ttttatcaag gaattccatt tctttccgac cgtcttcccg tccatctgaa agaggcctat
    30601 cccgccagca ctggcattgg ggccgacggg cattatctca tcatgagttg cattgccctt
    30661 cagacgccgc ggacagatat cgtcgcaatg ccgctcgaaa acccccgaca aacaccggcc
    30721 tttggttacg ggtgccagta cgggatcaaa agtcctaagt tctccagggc aatggcgctt
    30781 tcctgtgggg cgtatgcaac cattttcatt tcgggcactg caagtatcat caactccgaa
    30841 acacagcata tcggggatgt ggaacggcag acggatgaaa cgctggacaa cattgccgca
    30901 ctgattagcg aagagaactt gagccgccac tatttgccgc aattgggcac aacattagac
    30961 cagttggcac ttgtgcgggt gtatatcaaa cgggctgagg actaccccaa ggtccgcgcg
    31021 atttgcgagc gaagacttgg gaatgtcccc accatttacg ccgtggccga cgtctgtcgt
    31081 ccggaattac tggtggaaat cgaggggatc gcgttttccc agcgagggtg tcaatcgggt
    31141 caatgctaaa aaatcttgtc gggtaaagca ggaacccctc acaccggcga tgatcggctg
    31201 ttcacagcac ggtttcgaaa atcttggcaa acgccttgtc aatgtctgcg taggcgaagg
    31261 aaaatccgga ttcttgcaat cgtaccggta taacgcgggc acttgctaaa agcacctctt
    31321 gggcaagctg tccgaaaagc agtttcaaca ataatgttgg gacgcgaaga actgccggac
    31381 gatggatcgc cttggcaaag gccttggtaa agtctcggtt acgcaccgga ttcggactcg
    31441 ttatgttcac agggccgcgt atttcggcat gatccaggag aaactccacg gcacgacaaa
    31501 catcttcgag gagaatccaa ctccaccatg cgaggccctg gccgatcggg ccgcctaagc
    31561 caagacggaa caccggcaga agcgaggcaa ccacaccgcc atgccgggca agaacttgtc
    31621 caaatcgcaa attgaccaca cgaacaccat actcctcagc atagcgggcc gtggcttccc
    31681 actcctccac aaccgtagcg agaaagcccg tcccccgggg cgatgcctcc gtcaggactt
    31741 cctcgccgcg gtcaccgaaa taaccaatgg ccgaggcaca cagaaaaaaa cgaggcggcg
    31801 atgcagtctt ctgtaaggcc tctaccaata gctgggttga atcaatgcga ctgctacgga
    31861 tcagttgctt atatgagcgc gaccagcgac gtgccgcgat gctcgcgcct gccagatgga
    31921 taataccatc cagaccctcg atggtcctaa cgtcgatcag ctttcgttcg ggatcccagg
    31981 agatttcgtc ggctgcctga ggctcccgcc gtacgaaacg acgaacctca tgccccaacg
    32041 acctcaagtg attgacgagc gcactgccaa tcaagcccga gcttcccgtc acggcgatcc
    32101 gcatcatctt gcggtactcc atgaaaatac cccatcctgc gcaattaagc aggcctgggg
    32161 tgtgttgcca tcgacgtcaa ggaaagcgat ggatcaatct ccatcagtcg gtgtggaaca
    32221 cctccggaat tggcttccac catttgcctt caggggtatc cggcaagcgg atttggcagg
    32281 gatcggtttc cttccaccag cgttgggttt ccggatcagc ggcgatcttg gccatgtcgg
    32341 cttcaaagtc gtcaccaacg tactcgaaat agctgaatag gtaaagtttg ccttctagtt
    32401 cagccagata aatcgaatag ttgcggatgt tcgaatcgcg aattcgctta atgacgcctg
    32461 gccaggtgtt ggcgtggagc tggatgtagt aatccttctt ctcaggacgt aggccgatga
    32521 cctggccgac acgcataact ctcttgactt cgccggtagc gg
//
LOCUS       DAOSUT010000024        32169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_962_length_32169_cov_9.627172, whole genome shotgun sequence.
ACCESSION   DAOSUT010000024 DAOSUT010000000
VERSION     DAOSUT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 32169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32169
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_32169_cov_9.627172"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..972)
                     /locus_tag="PLD05_03600"
     CDS             complement(88..972)
                     /locus_tag="PLD05_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP76545.1"
                     /translation="MTARLTRRSALGTLAGTVAAASLSTRVTAAGQDSPAPLKGNINH
                     SVSRWCFGKVPMEELCAFCKKIGIKSIELLGPKDFPIIKQFGLTCAMVSSGTSLTSGF
                     NKRENHDGLVAKYEEMIPIVAEAGFKNLICFSGNREPGLDDETGLKNCEVGIKRILGL
                     AEKHKVILVMELLNSKVNHKDYMCDHTAWGVELCKRIGSENFKLLYDIYHMQIMEGDL
                     IRTIRENIQYIGHFHTGGNPGRHEIDETQEIYYPAVMRAIVETGYTGFVGQEFVPSNP
                     EPAAALASLEKCIRICDV"
     assembly_gap    1361..1460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1461..1721
                     /locus_tag="PLD05_03605"
     CDS             <1461..1721
                     /locus_tag="PLD05_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76546.1"
                     /translation="QKTLLEALAVEPRNVRALNALGHLREISGEKELALAHYEQSLQV
                     NQKQPELALRVSALRNELRVAQSTSPTQVSSNPWVPRTNRVR"
     gene            1871..3226
                     /locus_tag="PLD05_03610"
     CDS             1871..3226
                     /locus_tag="PLD05_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76547.1"
                     /translation="MLFRKLSLLLSAGLLLGLGVSLSARGEASFQQPAPRHVVVLLRN
                     RNVIEGELVAEGDIYRIRVSQGEIFVPHREVLAVADSIKALYEHERSRLFGREAEPHL
                     EMAIWCAEHGLEEEAWFELSQARLIDPRHPGLPLATRRVEMALLRKSQLSPSGKTRDE
                     STRAQASRAAQNAEAGARGENDKLGLPQVPDPNALFRGLPPGTGEQFVRVILPIVNHS
                     CASAGCHSKPDMNPQIRLLRVPTSRPPLRGEIAQNLQTVLGWVNFEHPGASPLLQVPL
                     EPHGGSPVPLFSGTAMKQYRELVDWVYAVTRRQAPPPPPGESSDAEAATLTGCSPPMS
                     AAGAAGVSLDGAFPILDFDVQQASHEISADSRGPKVVPATGSSPVNPVGAGGAVSAAT
                     PSDNPAGKPLPLLSDRPLFQSNPEPMPGIRWMFARPGSFPPLGSGVVTPELSPASSED
                     R"
     gene            complement(3252..3749)
                     /locus_tag="PLD05_03615"
     CDS             complement(3252..3749)
                     /locus_tag="PLD05_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOP76548.1"
                     /translation="MISADDNPGAHSDSPNIDGNPMNQPTGTIRKRGVVAVIAEEGKL
                     LLIRRAANVPAPGKWCFPGGAIEPGETPEMALIRELREELGVDIKPTRVLWESVTPWG
                     VFLMWWQASLPSNARLTPNPKEVAEVFWMKPHDLVAARDLLDSNRQFLEKVLSGEITL
                     DLDGC"
     gene            3936..4433
                     /locus_tag="PLD05_03620"
     CDS             3936..4433
                     /locus_tag="PLD05_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOP76549.1"
                     /translation="MGSENIPFDANEHGPSSEPPLVQGFIVFLESGISQLNNLLRRFD
                     VSRCRVLALHVTSQCGAAMVRIIVSHPEEGRIILERAGLPIADYRLVGVVLPDGVQPL
                     LRVYLPLIQANISVTATHVVPLTGRGKNAVLLEVDAPSRTIEILLAHGFQLIDEKMLA
                     QESPS"
     gene            4490..5908
                     /locus_tag="PLD05_03625"
     CDS             4490..5908
                     /locus_tag="PLD05_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOP76550.1"
                     /translation="MRVSTRRLFSTRLVIFVVASVLAQLSLVNGELARAVEDQATAEQ
                     YAQLAEEIEGHFLKEVLPFWFPRCLDQEYGGFRPHFRSDGSLGPLNDKTIVFQSRMTW
                     VAAEVAQRYSELAETYRNYARHGLKFLRTVMWDREYGGFYWGVDQNGQVKPSYGQEKH
                     LYGISFGLYAAANVYRATGDADALQLAQDTFAWLEKHAWDRKHGGYWEAFARNGTPLI
                     SPPPPGSDGRANPQRDLLGTPYGFKSMNSHIHILEALTELHRAWPSEEVTNRLREVFF
                     IVRDKIAVEPGCLNLYFTPDWRAVPDHDSFGHDVETAYLLLEAAERLHLANDPATIHR
                     AKSLVDHALDYGWDNRLGGFYDKGTAFGQPYGLEKIWWTQAEGLNALLLMHELFGDDD
                     PRYWPQFVKQWQFIKQYQIDHNTGEWFDTVSAEGQPQIKDLGHIWKAAYHNGRALMEC
                     GTRLRRLAGMNGLTPHEKSGKR"
     gene            6221..8119
                     /gene="dnaG"
                     /locus_tag="PLD05_03630"
     CDS             6221..8119
                     /gene="dnaG"
                     /locus_tag="PLD05_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOP76551.1"
                     /translation="MELPFAGAVIEGLPTWEPISTMPLTPYSDVKEQIRQAIDIVDLV
                     ERYIPLRRQGRGFVGLCPWHDDHRPSLQVNPQRQSFRCFVCDIGGDIFTFMMKIENVE
                     FPEAMAMLAEMAGIPLPRGIGRGSAVSQQKQDLYRVMAWAEQQYHQCLLEAPEAAPAR
                     EYLASRQISPASIAQFRLGFSPLQPDWLLRRARRDQIDPKDLVTTGILVGTPGRDLID
                     RFRGRVLFSIRDSQGRTVGFGGRILPHMAEQVPAKYVNSPETPLFSKSRLLYGLDVAR
                     DAFRRTGTALVMEGYTDVIMAHQCGFHNAVAVLGTALTDAHLQVLQRFVDRVILVLDG
                     DEAGRRRAAQVLELFVARQIDLRVLTLPDDLDPCDFLLKYGAPGFQDALDTRTVDALE
                     HAFAAATHGVDLERDIDGVTRAVDRLLMVMAKAPAKLGQHTAQQQAREWKILSRLSVM
                     TRIDESVLRKRLEELRRGGRSRTAAVATPAPKTTLDGWHRTFLEFLVTFPDAGEKLRA
                     AFQPEQIEIPLVRRIFEIALDLLANGKPADFDHLMLAVDDPLVHNILVDVDQTASEKR
                     LAEQNPEEILRGLLRAHEERVARARGSQLTHVLRQGGLDPEQQIDLLKRIVEQERNRQ
                     KWSEPMDG"
     gene            8230..9888
                     /locus_tag="PLD05_03635"
     CDS             8230..9888
                     /locus_tag="PLD05_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP76552.1"
                     /translation="MKHGDKELRRLIAQGRAQGYLTYDQVIEYLPDQGSRPEKLDRLI
                     TALEEAGIEMVNRPPRSETSANAAGEPQDEDPLLKDPLLRDLLPGEKAKWNSDPIRLY
                     LSQMADIPLLTRQQEVALAKKIEVTRKRFRRTVMGCHLVLKQAVQTLEAVQRGDLPFE
                     RTLKMSLTDQLTKNQILGRMPHNLRTLRALMAENEKDFRRMINRRTPEEVKAKARRRF
                     LARRARMLILVEELGLRTRRVQSMMQQMRDILKRMETIRARLQELKTQPGELGERERL
                     RRELMALMLCTQETPRSLRIRCEKMETQFRDYSEAKRELCNSNLRLVVSVAKRYRNRG
                     LSFLDLIQEGNTGLMRAVDKFEYRRGFKFSTYATWWIRQAITRALAEHSRTIRIPVHI
                     IDQIGKLKNTSRRLMHELGREPTPEEMAQATALPAEDASRLLEMGRQPTSLDRPIGED
                     EDNCFSELLADHTLDKPENIAHRDLLREKIDNLLKTLTYREREIIRLRYGLGDGYNYT
                     LEDVGRIFKVTRERVRQIEAKAMEKLQHPVRAQQLAPFVDGLVG"
     gene            9976..10713
                     /locus_tag="PLD05_03640"
     CDS             9976..10713
                     /locus_tag="PLD05_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76553.1"
                     /translation="MSSQALTIPEILRRLHGLHQQMDQLRGRLMKGPRLAQAHQEQIR
                     QAENNLDQAKKAYNDTRLASHRKQLEYEQKLADIQRRKGRLLECDDNREYQLLKDQIA
                     ADEMAASVLADEAIELMTRVEEMAVKVQEAEAALAAAKDAAAKWQAEFEKQEPVIRQE
                     IAQVEQELKHLETLLTGDVRELYQRALRNKGSSALAPLRGEFCGGCNQHVPLQMRSQL
                     LLGNAIWCRSCGRLLYLPENENPHSRS"
     gene            complement(10710..12716)
                     /locus_tag="PLD05_03645"
     CDS             complement(10710..12716)
                     /locus_tag="PLD05_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOP76554.1"
                     /translation="MVRTKDILGPEGRIAVRLPGYEHRPQQLQMAEAVERAITSQRPL
                     IAEAGTGVGKSFAYLVPTILAVAGELDAHDIQRAVISTHTISLQEQLLNKDIPFLRSV
                     VPYEFTAVLVKGRGNYISLRRLATALRRSRTLFADEEWEQLQAIQQWAAQTTDGTLSE
                     LSFRPFATVWDEIASDKDNCMGSRCPHYNQCFYFAARRRAQRAQILIVNHALLCVDLA
                     LRREGANILPEYDLLVIDEAHTLESVASEQFGTEVSNTQIMYNLRKLYNERTQRGLFV
                     HYRSPEGQKAVMDCLYRCDEFFAQVFDWYEKQKPGNGRVRQRDVFRNTLTEGLQKLAS
                     IVHKEAARIEKEEERQDLVAVRDRLQSLATQVDQWIRQSHEGYVYWVEVQEARQVPRI
                     ILGAAPIDVGPILREHLFQEIPRVILTSATLAVAESGFEYFKSRIGLTRADTLQVGSP
                     FNYQEQAELIIVRDMPDPSLDSSQFEKATVVALKRYIARTEGRAFVLFTSYDFMRRIA
                     AALQSWFAKENYTLFSQAEGLPRSQMLERFKTTPRAVLFGTDSFWQGVDVPGEALQNV
                     IITKLPFAVPDRPLVEARLEAIKAAGRSPFQEYQLPEAVIKLRQGFGRLIRSRADRGI
                     VVILDPRVYQKSYGRVFLRSLPPCRIKEEQLFDSGRLPACDPSS"
     gene            12954..13547
                     /locus_tag="PLD05_03650"
     CDS             12954..13547
                     /locus_tag="PLD05_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76555.1"
                     /translation="MAIEFYCSQCRNRLRVDDSAAGRQARCPVCNAIMIVPAVGVIQE
                     PPGAVRSEFEGYIPSVGPTRVSPPPPGTMGGTTLGSGRPGPEPATYQPYVTPQHYGEV
                     VGRSAEKREEGWGGAALVLGLFGLIAWCCPIIGVVIGGLALVFGIVSLGKGGGPMAII
                     GLILGIICLVLSIFNALLGMALMLADAEVVSLVMTDL"
     gene            13780..14778
                     /locus_tag="PLD05_03655"
     CDS             13780..14778
                     /locus_tag="PLD05_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76556.1"
                     /translation="MPITFQCPACYAMLKVPDDTVGKQAKCPRCGQIVTISAGAPAAP
                     SSDMGAAVPPPIGGEPPRPQEPTSVNPYAPPQAQTSYLAPIDRGTIEPQIISFSETFN
                     VTWRLFFDNIGPFLGMGALLFGLTFVGNMFVNFISLFFAAAGDDEAMIFLGIIVLIFS
                     VLMAAVSMWLQGGMLRYSVELAKGMRPNFGRIFLPVNQFLGYFAVVILLSLIVTAGFI
                     LLILPGIILMLMLFCAPAVYLDGKAGVIESFSVSRTITRGNRLTIFGLVLVNTILAFL
                     ATVLTCGLAGILAPAFFSLLTAVIYTRSAGLFASARPPMVQMPPPSWMPGPSPGLR"
     gene            complement(14873..>16076)
                     /locus_tag="PLD05_03660"
     CDS             complement(14873..>16076)
                     /locus_tag="PLD05_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76557.1"
                     /translation="NSCMVEGTNMSHRVPHLGLRYLFVFSGLLTPVLFAYLIFSPGAL
                     LGAAQQASPDYVAPLVLFEGKADATKVEQPSTSPSGRSQGHGKKQRPQTAEGVDESAG
                     KTSSGEQKANRPQFVRLLVRKNEPQYLQTAIVSYVVRDDSSQGKTLSAGETGRSQPAW
                     PKDLRVDLVGVIHIADRGYYEQLNKILRKYDVVLYELVAPEGTRVTPGQRPAHPVSAL
                     QQSLTNMLGLAYQLDVIDYHRPNFVHADLSPEEFYQSMKEKGESTWAMLLRAFGYEWA
                     RSLSRQSTPNDADLVRALFSKDRQLQLKRLFAQQLSEDVEGQIRALEGPQGSTLISGR
                     NERVLAVLEDQAKKGAKSVAIFYGAGHMPHFHDRLTQIGFAVEKTRWLNAWDLRSPVA
                     DSTERTGN"
     assembly_gap    16077..16176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16706..30697
                     /locus_tag="PLD05_03665"
     CDS             16706..30697
                     /locus_tag="PLD05_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76558.1"
                     /translation="MLANLWASLRQARNKSHSSRERRRLRVDALEERTLLSVTPVDIL
                     DKLVNQSLIPSGGTIAGQSLAVDADGDFVVVWTRYDTLTDPNTGLPIIDPLTGQPRTE
                     ANIYARYLTNEVQRITLPSGVLNDTVPTQYGRFSLIYGGTTLVQKLTISATYEPYVTT
                     QQNIAGTFVLQADANGDGIIAATERATIVYDETTDVNTLASTIQTALQGIGGPLQNAR
                     VSAVNAKEFVIEYGSAALGINQPKLSVGNVNFTSGFYPSVEVSTAREPILISNIPVSP
                     DNPALTAQALAQAFNTRLQAYALGPIDFPPPDRVPSATEGPYTDPLVAAVSIPQISVT
                     AVNATTFDIRFIGDSGKLDHPELIISAVSDELGNNLLPSPEATVTTLKQSSPEFRVNP
                     EEPDDPFTPGPDKYNQTNPAVAIDADGDFIIVWESEVPDSVAFASVSDIFARRFRPVG
                     TANLGVSQFIVDMDADGTPETPISGILPLVSPLKTVTQTLTVDAFLPGTLTGNFRLSL
                     AGRQTEDIAFDSTQLATTAANIQGALVMAGFQDVTVQVISSTDPYKFRVTFRQTFSGP
                     NGLADAILQYVDPSAGVPLSTRASVSSQLAPGSDLYTFQVNTETLRAQFAPAVAMDML
                     GNFVIAWANSAQDFSYYNGIKAQIFDRNGNKIGEEFLVNTEDTAVYLNPAIGMARTGE
                     FVIAWERTHDVNYLTGGAYATAVQAKAYNLAGQVILPQFGVGGGGDPAIAFDSADHFL
                     ITWQVVGDPDNTGQTTSGVRGIVYDLSGNTIRDVFRANSATFDPADDTLWPLYQGGAQ
                     GGLDADGDMFVVYDGYGPDVSQNVYGLTTAVQQLLRSRINATTNADLLNYFDPQNESL
                     LGAGLPSNGDVDGVIETILIRALNRGANAEQIGRLRAILDSVAGLLRGEANGVMVSRF
                     DASPTMAYTTLFSDSVVNTLRDGHNTRYLILMNQALNWESFIIRLYRDGFGGYEDVTI
                     NNIQTNQNWNITLTRDRIDELLEGAARTGTNWAETRYEGSIDVRILSAFEVASRIGTP
                     WEIPVANPNQYVIYEVTFQGEGHDWPFSMVGVWPGRAGGNDVPPPLMIVETYGDRGVT
                     QADASMGVTPEGSFVVIYTQYEQNTDGTVTNATIRYRQFQETADTAGPFVTDILAPTG
                     TSLWGGATVQGPVNQIVVTFNEELLGGDPQSVLDSVLNPENFVLYRNGVEIPRGIVKV
                     DFGMNAASLLNGKAYDLDGDGVPDGTYQLYALPTNKWEAVLTVDGNGALEAGTPGLGD
                     GDYQIVLRTPSGATSGLRDKAGNPLNSTGFLPAGANTSLSFHVTVSAQDQPVLDLNAR
                     TQPESPNAVARDADGDYVVVWTQTDASGRNRVYFRLYDRDGTPADLDVNGNGVIDPFE
                     IDNAPATPVTTSSTFAGDDQQYASVAMTPDGDFVITWTNTRSGNADIYARRFSANGQA
                     LGEAFLVNTVTANDQKWSDVAMDAEGNFVIVWSSWGQEPGGVGNRWGIFARRYDQLGQ
                     PVGLEFQVNTIVAGDQVLPSVSMDYRGNFAVAWQTAGEIALRTFDATGVAREAAEILL
                     GQTIVGQKAYPDVALAASGQYVVVSWAGPDGNGNGVYVDLLQWDPAGLVWTPVGTSQL
                     VNTTVVGEQSYASVAMAVNGSFVVSWSGIGTQVGQEDQLESGVFYQQFSNALTKVGGE
                     RRVNQVVAGKQWAPSVGADADGNFIVVFTGPSAVNPALTTVYRFDSRVYLNQGDVSAP
                     IVTKVLTGEGVLVTEGARVAPGPQELVVVLSEAMSERLADINGDGLIDNRDGPAPDSV
                     LNVQNWKLYRDGAEVAGAIKSVTFRYNALTRRYEAVLGLDSNPVTTAIEPLVPGEYQL
                     VVKDVMTDAYPYFRLTDPVTGRQLDGDRDGVAGTAGGAAGFVRNFMVIGGVPGQDVSV
                     IPGVPTQNARTQPESPNAVARDADGDYVVVWTQTDASGRNRVYFRLYDRDGTPADLDV
                     NGNGVIDPFEIDNAPATPVTTSSTFAGDDQQYASVAMTPDGDFVITWTNTRSGNADIY
                     ARRFSANGQALGEAFLVNTVTANDQKWSDVAMDAEGNFVIVWSSWGQEPGGVGNRWGI
                     FARRYDQLGQPVGLEFQVNTIVAGDQVLPSVSMDYRGNFAVAWQTAGEIALRTFDATG
                     VAREAAEILLGQTIVGQKAYPDVALAASGQYVVVSWAGPDGNGNGVYVDLLQWDPAGL
                     VWTPVGTSQLVNTTVVGEQSYASVAMAVNGSFVVSWSGIGTQVGQEDQLESGVFYQQF
                     SNALTKVGGERRVNQVVAGKQWAPSVGADADGNFIVVFTGPSAVNPALTTVYRFDSRV
                     YLNQGDVSAPIVTKVLTGEGVLVTEGARVAPGPQELVVVLSEAMSERLADINGDGLID
                     NRDGPAPDSVLNVQNWKLYRDGAEVAGAIKSVTFRYNALTRRYEAVLGLDSNPVTTAI
                     EPLVPGEYQLVVKDVMTDAYPYFRLTDPVTGRQLDGDRDGVAGTAGGAAGFVRNFRVV
                     SPPSPPAPDVPVVPVAGDAILRTQPESPNAVARDADGDYVVVWTQTDASGRNRVYFRL
                     FDRDGTPADLDLNGNGVIDASEVNNAPATPVTTSSTFATDDQQYASVAMTPDGDFVIT
                     WTNTRSGNADIYARRFAASGQPLGEAFLVNTFTVNDQKWSDVATDAEGNFIVVWSSYN
                     QLDIPGAQNTWDIFGRRFDRFGQPLGVEFLVNVTTTGDQTLPSVAMDIQGGFVVAWQC
                     NPGREGKDVYARSFWPDGSPQAEGVGAGWYYGEFLVNQTLAGDQVSPDVAMSPSGDQF
                     VVTWTGPDSDSSGVYFRQFSRTVDIFAPLRRVYNSNISAPGLRLGHPVISTGPYTVQV
                     PYTVVDSITINDVAQIQDLNVTINIQHRRASDLLIELRHVDTNTEIVLSNRNPQRPAG
                     TYASGANYINTTFDDEAATTIVTGSPPFTGSFVPMPDALSAFDGQSLAGTWELVITDM
                     YYDYNNDPRDWPFVLDGYLISWQLSFATVPPSSAEILVNETTAGPQFAPSIAMAYDGS
                     FVITWTGTGLVPSQEDISGTGVFYRKFDALAAPTTGETRANNTIQGDQRFSSVAMDAD
                     GNFIIVFTGPVASAPGLTTVFQFDSRVHLLANELIPFEPDSAAPLVTSVQTNDGRPIY
                     TNAILLQPVTQIVVAFSENLNTDLLDTDGDYVLDTPGLSSILNPKNFELIKDGQSLPF
                     SVIAVDFGWNAVSRKYEARLTLDGDTVMPGVQALGKGVYALRISDAVVDLVNHRLDAD
                     RDGTPGDASGQGYYQIDFAVGPLSQTYEHLVNQSVEYVQAFAVSQGPGLAVDRNRKTL
                     AVDAEGNFAVVWMSFGQDDRSDSTGAGVYVRVFDRAGVPITPEILVNQTVVGSQTNPV
                     IAMDADGDFVVVWESQDNVVDGSRGIWARLFNSVGQPLTDEILVNTLTAGDQYSPSVA
                     MDDFGNFAVVWATRAQPFSYYNDIKGQLFSYRGEKIGSEFLVNEQNIAGTTLLPGSNE
                     VHPAVAMDPQGNFVVAWEQVVQQRNGVGLDTVIVGRLFQSNGAPLTGEFAISQGDTGL
                     VDPEHDTDDASGGADIQRRARNPQVIMDRLGNFSVVWEAYQDNDIGQQGADSYGIYFR
                     RFNSDATPADDEHQANLVVTETTQGAVAPGIPSRYFAYNQLNPSIAMDANGQYVVVWD
                     GNGSAPHVLSVTDKLYTDWDRDGVWIRQFQATTAGGDLEFVSPQTRVPYTSYGPQQFP
                     SVVKTLDGDIIVVWSGRGIGDPNGIFFRKYTSIDAAGPIVTDVYAGEGIRLVGSRSGN
                     VRVSQPVNTFTVVFSEDMMRTGPDSVTNPQNYLLIKDGSEIVAIKSVTFGLNPQTNKW
                     EAVLTVDSDSLAPGDQPLPSGNYELRVLSPVLAAGGTPGRSGVRDKVGNALNESGLSQ
                     GGQNFTLNFQVVVVGTSPGTPGTGATDFAVNTTQADDQTEPVVAIDSRTGNVIVVWTS
                     RSTEQYPDGSTALATDILCQIYNSFGEPIGPEFVVNNDTIGNQFQPAVAVDGLGRFIV
                     TWAGEGQSDADGGIWARIFDSQGSALGQQFRVNQFRQYTQHSPKVATDSAGNFVITWS
                     SYGQSDDIDAVYARLYTQSGQAKGNEFRLNTTTAGYQRNSDVAMDDAGNFVAVWMSDG
                     QDGSAHGVVGRVFNANGTARTGEFLVNQYTPNEQRDPRVAMNGAGDFVVTWTSYGQDG
                     SGYGVYARCYGSNGVAKGNEFRVNQTTLYWQFQPDVAMDAQGNFTIVWTTTGQDNDLA
                     NDNGIYARMFAADGSDRSIPNLGKLGEFRVNAFVTGNQNTPAIGMDRAGNFVVAWSGP
                     STDPDPIVAATNGTDVFARWFQLPSLLRMSQTANELVIEGTNGNDVFEFTAGTSLTKS
                     TLKVNGQTVSIPSGVSRVVFDAKGGQDKITITGTSGADTATFSPTQTTFEFGSSQTLV
                     VLNGEQVKIDGKGGTDALRVYDSPRTDNYTATPTRVTGSDNLSSYSLEATNFENVVLT
                     SRAGGEDVAMLYDSPGDDTFTATPTYGEMVGTGYKLRAEGFAFLHGYASAGGTDTAML
                     YDSPGDDEYVCTPDYNILRGENFYLRAKYFDYVHAYATAGGYDKATIKGSAGNDKINL
                     DQKVVRLNSGSYVHRGKFFEEVTVRGCGGQDTAYVTDGIVLPGTVSRGTPPISLDASQ
                     IVYLRQLYKIQTTNEGGNGTNRTIRPAADAVFTAYWP"
     gene            31317..>32169
                     /locus_tag="PLD05_03670"
     CDS             31317..>32169
                     /locus_tag="PLD05_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76559.1"
                     /translation="MYCQRKAMRTIVGVFLLALATPALAQVKDMQYFAPAQVENFGGG
                     RRANEGFFISGEYLRWSISKPDVTSVGYPGLTRQVYYDTDISREQTNTLDTSFIGAKF
                     TDGSRVDLGYIEGHEGWLFSGTFLSTLGRTHGVASADIVFADKEFGDPPRYYLQGYVD
                     DALTNIQNLPVRFTAPYIEDIEADEGGDPDSLPEPSWGVMMENLTEHWSTELNYIYRC
                     HPTRRGATWELFLGVRYFELNEDFNVWAPYSYVPYDPTADENDPAGVGFPTRVGILAD
                     SYWFTKAE"
BASE COUNT         6941 a         8374 c         9424 g         7230 t
ORIGIN      
        1 gccggttttc tcaatcgatt ccgacgttcg cttgccctca tcccaaccct ctcccggagg
       61 gagagtgaga ctttttggga tacttaatca gacgtcacaa atccgaatgc atttttccag
      121 ggacgccaag gcggcagccg gctccgggtt actggggaca aactcctggc caacgaagcc
      181 ggtgtagccc gtttccacaa tcgcccgcat cacggccggg tagtaaatct cctgcgtttc
      241 atcgatctca tgtcggccgg gattgccacc cgtgtggaag tgaccaatgt attggatgtt
      301 ctcgcgaatg gtccggatca gatcgccttc cataatttgc atgtgataaa tgtcgtaaag
      361 gagcttgaag ttctcggagc cgatccgctt gcacagttcg acaccccaag ccgtgtgatc
      421 gcacatgtaa tccttgtgat tgaccttgct attcaggagc tccatgacga ggataacctt
      481 gtgcttttcc gccaggccga gaatccgctt gattccaact tcgcaattct ttaggcccgt
      541 ttcgtcgtcc aggccaggtt ctcggttacc cgagaagcag atcaaattct taaatccggc
      601 ctcggctacg atcgggatca tctcttcgta ttttgccacc aaaccatcat ggttttcccg
      661 tttattgaaa ccactcgtca aactcgtccc cgaggacacc atggcgcatg tcagaccgaa
      721 ctgcttgata attgggaaat cctttggccc caggagctca atcgacttga tgccgatctt
      781 tttgcagaag gcgcatagct cttccatggg gaccttgcca aaacaccacc gggagaccga
      841 atggttgatg tttcccttca aaggtgctgg ggagtcttgg ccagccgcgg tcactcgagt
      901 gctcagcgag gcagccgcca ctgttccggc aagagttcca agggctgaac gacgggtcag
      961 acgtgctgtc atgggagttc tcctatcctc gagaaagtgt ttgtgtagca cctaagagcc
     1021 gctaagactg ttcaggtggt caaatgaacc agactcattt tacacaacgg ggcagatgac
     1081 tcaaataacc catttttcgt agggtttcgg tttttcggtt aaatgaatgc atttaccggt
     1141 caaaaaactg tcttgcccca aaaagggcct attgtgtatc ctcccgtggt ttggaaatga
     1201 ttgttggagc agggtttcag tgatggccga ggacgtccgg tgtggtagct ccccatttcg
     1261 ttgaacccga aagaacgtgt tattcgcggg gccttatgat ggtccttgcc gccagccttt
     1321 tatgccttgg cggctgcaca agtatgactg cgcagtcact nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn caaaagacgc ttctagaggc ccttgctgtg gaaccccgta
     1501 acgttcgggc actcaatgcc ctggggcacc tccgcgaaat aagcggggag aaagaattgg
     1561 cactggccca ttacgagcaa tcactacaag tgaatcaaaa gcaacccgag ttggccctcc
     1621 gggtctcggc cttgcgtaat gagctgcgag ttgcccaaag cacctcaccg acccaggttt
     1681 cctcaaatcc ctgggttccg cggacaaatc gcgttcgata gtggcgatgg ccacgctctt
     1741 ttcgatgggt aaagaatcga cattgaccgc cagccgcttg gttggctacc tttcccgcta
     1801 ggcagcggga aagaaatgaa aaccccggct cgttagtcca tgacgcaccg caccatccga
     1861 gggggaccat atgttattcc ggaaactcag cttgctcctg agtgcaggac ttctgctagg
     1921 gctgggagta tctttatccg cacgggggga ggctagtttt cagcagccgg caccacgaca
     1981 cgttgtcgtg cttttgcgga atcgcaacgt catcgagggc gaattggttg cggagggcga
     2041 tatctatcgc atccgtgttt ctcagggcga aatttttgtc ccacaccgtg aggtcctcgc
     2101 cgttgccgac tcgatcaagg ccctttacga gcacgagcga tcacgactct ttgggcgtga
     2161 agccgaaccg catttggaaa tggccatttg gtgcgcagag cacggactgg aagaggaggc
     2221 ctggttcgag ctttctcagg cacggttgat tgatcctcgt cacccggggc tgcccttggc
     2281 aacgcgccgg gtggaaatgg cccttctccg aaagtctcag ctcagtccct ccgggaagac
     2341 acgggatgaa tcaacgaggg cgcaagcctc aagggcggca cagaatgccg aagcgggggc
     2401 gcgcggcgaa aacgataagt tgggcttacc ccaggtccct gatcccaacg cgctttttcg
     2461 cggtctgcca ccggggacag gggaacagtt tgtccgtgtc attctgccga ttgttaacca
     2521 ttcttgtgcc tcggccggat gccacagcaa gccggatatg aatcctcaga ttcgtctctt
     2581 gcgggtccca accagtcggc cacccttgcg aggggaaatt gctcaaaacc ttcagaccgt
     2641 gcttggctgg gtcaattttg aacatccggg ggcaagccca ttgctccagg taccgcttga
     2701 accccatggt ggctcgccgg tgcctctctt ttctgggacg gctatgaaac agtatcggga
     2761 acttgtcgat tgggtgtatg cggtgacccg tcgccaagcg ccgccaccgc cacctggcga
     2821 aagttcagat gcggaggccg ccaccctaac cggttgtagt cccccaatgt cggctgccgg
     2881 tgccgcggga gtttctctcg atggcgcgtt ccccatcctc gatttcgatg ttcagcaagc
     2941 gagccacgag atatctgcgg attcaagagg ccccaaggtg gttccggcaa caggttcctc
     3001 gccagtcaat ccggtcggag cagggggggc ggtctcagcc gccaccccaa gtgataaccc
     3061 agcgggaaaa ccgctgcccc tcttgtctga tcgtccccta tttcagtcca atcccgagcc
     3121 gatgcccggc attcgctgga tgtttgctcg tccagggagt tttcccccgc ttggttccgg
     3181 cgtggtaact ccagaattgt ctccggctag ctctgaggat cgctgaggat cacctcctgt
     3241 gtgccgcgcg attagcaccc gtcgaggtct agcgttattt caccagacaa gaccttttcc
     3301 aagaattgcc ggttgctatc aagcagatcg cgggcggcga ccagatcgtg aggtttcatc
     3361 cagaagacct ccgcgacctc ctttggattc ggtgttagcc ttgcgttcga agggagagac
     3421 gcctgccacc acatgaggaa gacaccccag ggtgtcacgc tttcccaaag gacccgtgtc
     3481 ggtttgatat caacccctaa ttcctcgcgg agctctctga taagtgccat ttccggcgtt
     3541 tcgcccggtt cgatggcgcc cccaggaaaa caccattttc cgggagcggg gacgttcgcc
     3601 gcccgacgga tgagaagaag ttttccttcc tcagcaatga cggccaccac cccgcgcttt
     3661 cgtatcgtcc ccgtgggctg attcatgggg ttaccgtcaa tattgggcga atcggaatgc
     3721 gcaccggggt tgtcatcagc agaaatcatg gaattcgttc caagttggaa aaacaaatca
     3781 gggcatgaaa aaactctagt cgatttgggg aattcgccag tgacttggat tgttccgcaa
     3841 ggggcggaaa caagataaac taagcagaat catcatccat acataatagc gggttgcgtt
     3901 cccgctgttc aggtcttcta gtacgagcgt atcccatggg ttccgaaaac atccccttcg
     3961 acgcaaacga acatggccct tcgagcgagc cgcccctggt tcagggattc attgtttttc
     4021 tcgaatccgg gatcagtcaa ttaaacaatt tgttacggcg gtttgatgta agccgttgtc
     4081 gggttctggc gctacacgtg acgtctcaat gcggggccgc catggtcagg atcatcgtca
     4141 gtcatcccga agaggggcgc atcattctag agcgggccgg gctgcccatc gccgattatc
     4201 ggcttgtggg ggtcgtcctt cccgacgggg ttcagccact tcttcgggtg taccttccgt
     4261 tgatccaagc aaacatatcg gtcacggcca cgcacgttgt gcccttaact gggcgtggca
     4321 agaacgccgt attgctggaa gtcgatgccc cttcgcggac aattgagatc ctcttggcgc
     4381 atgggtttca attgatcgac gagaaaatgc ttgcgcaaga atcgccctcg tgaaaggatt
     4441 gggggatcgg gagtgacgtg tggtctgaca aacgatggga gcctcaacga tgcgtgttag
     4501 cacaaggaga ttgttctcca cgcggcttgt catctttgta gtcgcaagtg tgttggcgca
     4561 actatcgctg gtcaacggcg agctggccag ggcggtagaa gatcaagcga ctgccgagca
     4621 gtacgcacaa ttggccgagg aaatcgaagg tcattttctc aaggaggttt tgccgttttg
     4681 gtttccgcga tgtctggatc aggagtacgg cggctttcgt ccacactttc gtagcgatgg
     4741 ttctttgggc ccgctcaacg ataagaccat cgtcttccaa tcacgaatga cgtgggtggc
     4801 agccgaagtg gcgcagcgct attcggaatt ggccgagaca taccgcaact atgcccgaca
     4861 tggattgaaa tttctccgca ccgtcatgtg ggatcgggaa tacggcggtt tttattgggg
     4921 agttgatcaa aatggacaag tgaagccgag ttatggccaa gaaaagcatc tttatgggat
     4981 atccttcgga ttgtatgcgg cagccaacgt ttatcgtgcc acgggcgatg ccgacgcctt
     5041 gcagttagcc caggatacgt ttgcctggct ggaaaagcat gcgtgggata gaaaacacgg
     5101 cggctattgg gaagcctttg cacggaatgg cacgccgctg atttcaccac ccccaccggg
     5161 aagcgacggc cgggctaatc ctcagcgcga tctgctaggt actccctacg gcttcaaatc
     5221 gatgaacagc catattcaca ttttggaggc cctcaccgaa ctgcaccgcg cttggccaag
     5281 cgaggaagtt accaaccggt tgcgagaagt attctttatt gtccgcgaca aaatcgccgt
     5341 ggagccaggc tgcctcaatc tctatttcac acccgattgg cgagccgtcc cggaccacga
     5401 ttcgtttggg catgatgtgg aaacggccta tcttttgctg gaggccgccg aacgcctcca
     5461 tctggcgaac gatccggcga cgattcaccg ggcaaaaagc ctcgtcgatc acgcactgga
     5521 ctacggttgg gacaatcgcc tcggcgggtt ttacgacaag gggacggcct ttggtcagcc
     5581 ttacggattg gaaaaaattt ggtggacgca ggccgagggg ctcaatgcgc tgcttctaat
     5641 gcacgaactt tttggagatg atgacccccg ctactggcca cagtttgtca aacagtggca
     5701 gtttatcaag cagtaccaga tcgaccataa tacaggcgag tggttcgaca cggtgtctgc
     5761 cgaaggccaa ccccagatca aagacctggg gcacatctgg aaggcagctt atcacaatgg
     5821 tagggcactg atggagtgtg gcacgcggct tcgccgcttg gcggggatga atggcttaac
     5881 tcctcatgag aagtccggca aaagatgacc ttttgttggg ggaattttcg accttgaccg
     5941 ggagataaag gtctttctat aatcgcctgc ccgcgcgaag gtgtgttcgt ggcgaaagtc
     6001 gctccgcgaa ttagcgactg ggctagcgat tgcaatccgg ccggcgcccg cggcgaaatc
     6061 cggcacgggc tcagccaacc cacggtgggg caaaggcaaa caagggatgc ccacggtttt
     6121 gtttgacctg gggttgatgg ataattgaca ggaaaggtga cgcaaaagag gaaggttccg
     6181 tcaacctcaa tctgtgtgct cggaggcgga acggcggcaa atggaattgc cctttgctgg
     6241 tgctgtcatc gaagggttgc ccacgtggga gccgatttcc accatgccgc tcactcccta
     6301 ttcggacgtc aaagagcaaa tccggcaggc catcgatatc gttgacctcg tggagcgcta
     6361 tatcccctta cgtcggcaag gccggggttt tgtggggctt tgcccttggc acgacgacca
     6421 tcgcccgagc cttcaagtca atccccagcg gcagagtttt cgttgttttg tctgcgatat
     6481 tgggggggat atcttcacct ttatgatgaa gatagaaaat gtcgagtttc ccgaggccat
     6541 ggcgatgctg gccgaaatgg ccggtatccc tttaccgcgg ggcatcggca ggggttccgc
     6601 tgttagtcaa caaaagcagg atctttaccg cgtcatggcc tgggcggaac agcagtacca
     6661 ccagtgcctt ttggaggctc cagaggcggc accggcccgc gagtacctgg cgagtcgtca
     6721 gatttcccct gccagtattg cccaatttcg tctgggattt tcgcctctcc agccggactg
     6781 gttattacga agggctcggc gcgatcaaat tgatccaaaa gacttggtga caacgggcat
     6841 cctcgtgggt accccgggcc gcgatctgat cgaccggttt cgcggacgag ttttattttc
     6901 catccgcgat agccaggggc gaacggtggg atttggcggg cgaatcctgc cccacatggc
     6961 ggagcaagtg ccagctaagt acgtcaattc cccggaaacc cccctcttct ccaagagccg
     7021 cctcctttat gggctggacg ttgctcggga tgccttccgg cgaacgggta cggccctagt
     7081 gatggaaggc tacaccgacg tgatcatggc ccatcaatgc ggttttcata acgcggtggc
     7141 ggtgttaggg acggccttga cggatgccca tctccaggtg ctccaaagat tcgtggatcg
     7201 tgtgatctta gtgctggatg gggatgaagc cgggcggcgc cgtgcggctc aagtgttgga
     7261 actatttgtg gcacgacaga ttgacctacg ggttttgacg ctcccggatg atctcgaccc
     7321 gtgtgacttt ctcttgaaat atggggcacc gggatttcaa gatgcgttgg acacacgcac
     7381 ggtcgatgcc ctcgagcacg ccttcgccgc ggcgacacac ggtgtggatc tcgagcggga
     7441 cattgatggt gtcactcgcg ctgtcgatcg tttgctgatg gtgatggcga aggccccggc
     7501 caaactcggt caacacacgg ctcaacagca agcccgcgaa tggaaaatcc tctcgcggct
     7561 gagcgtgatg acgcggatcg acgaatcggt gctgcgaaaa cggctagaag agcttcgccg
     7621 cggtgggcgc agccgaaccg cagcggtggc caccccggca cctaaaacca ctctcgatgg
     7681 ttggcatcgg acgtttcttg agtttttggt gacatttccc gatgcggggg agaagctgcg
     7741 ggcagccttt cagccggagc aaattgagat tcccctcgtg cggagaattt ttgaaatcgc
     7801 acttgattta ttggccaacg gcaaacccgc cgatttcgac catttgatgt tggcagttga
     7861 tgaccctctg gtgcataata tcctcgtgga tgttgatcag acggcgagtg aaaagaggct
     7921 tgccgagcaa aatccagagg aaatcctacg tggtttgtta cgggcccatg aggagcgtgt
     7981 ggccagggcc cgcggtagcc aattgaccca tgtcttaaga caaggaggac tagacccaga
     8041 gcaacaaatc gacctactga agcgaattgt cgaacaagag cgaaatcgcc agaagtggtc
     8101 cgaacccatg gatgggtgag gcttttggcg tcaggaagct tgtgactccc gcaagcctgt
     8161 tgattcatcg gcttcctgaa aggagtgagt gctttctttc ggcacggaac gcgcgaaagg
     8221 agacgacccg tgaagcatgg cgacaaggag ttgcgtaggc ttattgctca aggacgagcc
     8281 caaggttatc tcacgtatga tcaagtcatc gaatacctcc ccgaccaagg ttctcgaccg
     8341 gaaaagcttg atcgcttgat cacggccctg gaagaagccg gcatcgaaat ggtcaatcgt
     8401 ccgccgcggt cggagacctc ggcaaatgcc gccggagaac cgcaagacga agaccccctc
     8461 ctcaaagatc ctcttttgcg agatctgctc cccggagaaa aggccaaatg gaacagcgat
     8521 cccatccgtt tgtaccttag ccagatggcc gatatccccc tactgacgcg gcaacaggaa
     8581 gtggccctgg caaagaagat tgaggttact cgcaagagat tccggcgcac ggtgatggga
     8641 tgtcatctgg tcctgaagca agccgttcaa acgctggaag ccgttcaacg gggcgatctg
     8701 ccctttgaac ggaccttgaa gatgtccctc acggaccagt tgacaaaaaa ccagattttg
     8761 ggccgaatgc cccacaattt acggacactc cgcgcgttga tggcggaaaa cgagaaggat
     8821 ttccgccgca tgatcaaccg tcggacacca gaagaagtga aggctaaggc tcgccggcgt
     8881 tttctcgccc gtcgggcgag gatgctcatt ctcgttgagg aactcggact ccgcacgcga
     8941 cgtgtccagt ccatgatgca acaaatgcgg gacatcttga aacggatgga gacgatccgg
     9001 gcccggctgc aagaactcaa gacgcagccc ggtgaattgg gcgaacgcga gcggcttcgt
     9061 cgggagctaa tggccctcat gctatgtacc caggagacgc cgcggagttt gcgaattcgc
     9121 tgcgagaaga tggagacgca attccgcgat tacagcgaag ccaagcggga attgtgcaac
     9181 agtaacctgc ggctggtcgt ctctgtggcc aagcgctacc gtaatcgcgg cctgagtttc
     9241 ctcgacctga tacaggaagg aaatacgggc ctcatgcggg cagtcgataa atttgagtat
     9301 cgccggggct ttaagttctc gacctatgcc acttggtgga ttcgacaagc catcaccagg
     9361 gcgttggcgg agcactctcg cacgattcgg atccctgtac acatcatcga ccagattggt
     9421 aaactgaaaa atacgtcgcg ccggttgatg catgagctgg gcagggagcc aactcccgag
     9481 gaaatggcgc aggccaccgc gttgccggca gaagatgcgt cccgcctgct cgaaatggga
     9541 cgtcaaccca ccagcctgga tcgaccgatt ggcgaggacg aggacaattg cttcagtgaa
     9601 ctgctggctg atcacacgct tgataagccg gagaacatcg ctcaccgtga tttgctgcgg
     9661 gagaagattg acaatctgct caaaacgctt acctaccgtg agcgcgaaat tattcgcctc
     9721 cgctacggtt tgggcgatgg ctataactac acgcttgaag atgtcggtcg tatcttcaag
     9781 gttacacggg aacgggtccg gcagatcgaa gccaaggcca tggaaaaact tcagcatcct
     9841 gtacgggccc agcaattggc gccttttgtg gacggactgg tcggatagta ccatgtccgt
     9901 cgtgggtgac ggatatcagg cgggctgtcc taatggacag ccctttttta ttagttatca
     9961 aggagatctc tcgccatgtc atcgcaagcc ttgacaattc ccgaaatctt gcgacgcctc
    10021 cacggtctcc atcagcagat ggatcaactg cgggggcgtt tgatgaaagg accacgatta
    10081 gcacaagccc atcaggagca gattcgccag gccgagaaca atctcgacca ggctaagaag
    10141 gcctacaacg acacccgact tgccagccat cgcaaacagt tggaatacga gcaaaagctg
    10201 gccgacatcc aacgccgcaa aggccgtctt ttagagtgcg atgataatcg cgaatatcaa
    10261 ctgctaaagg accagatcgc tgctgacgaa atggcagcca gcgttctagc ggatgaagct
    10321 atcgaattga tgacgcgtgt cgaagaaatg gctgtcaaag tccaggaggc tgaggctgcc
    10381 ctggccgctg ctaaggatgc cgccgccaag tggcaggccg agttcgagaa gcaagaaccg
    10441 gtcattcggc aagagattgc tcaggtcgaa caggaattga agcaccttga aacccttttg
    10501 acgggggatg tgcgagagct ttatcaacgg gccctccgca ataaagggag cagcgccttg
    10561 gcacctctca ggggcgagtt ttgtggcggg tgtaatcagc atgttcccct acagatgcgg
    10621 agtcagttgc tacttggaaa tgcgatctgg tgccgctcgt gcggacgact cctttatttg
    10681 ccagaaaatg aaaatcccca ttcgcggtcc taacttgagg gatcgcaggc cggaagccta
    10741 ccagagtcga aaagttgttc ttctttaatt cggcacggtg gaagcgaacg caggaagact
    10801 cgtccgtagc tcttctgata cacccggggg tccaagatga caacgatccc acggtccgcg
    10861 cgggaacgaa tcagcctccc gaacccttgg cgcagcttaa taacggcctc cggcaattga
    10921 tactcttgaa acgggctgcg tcctgcggcc ttgatcgctt ccaggcgtgc ttctacaagc
    10981 ggacgatcag gcacggcaaa gggtagtttt gtgataataa cgttttgcag ggcttcacct
    11041 gggacatcga ccccctgcca aaaactgtcc gttccaaaca gtacggcccg gggggtggtc
    11101 ttaaaccgtt ccaacatctg tgatcggggc aagccctcgg cctgagaaaa tagtgtgtag
    11161 ttttctttgg cgaaccaaga ttgcagcgca gcagcgattc gccgcataaa gtcataactt
    11221 gtgaagagca cgaaggcacg cccctctgtc ctggcgatat atcgcttcaa ggccacgacc
    11281 gttgccttct cgaattgtga agaatcaagg gagggatcgg gcatgtcgcg gacaatgatg
    11341 agctccgctt gctcttgata attaaagggg ctacccactt gaagcgtgtc cgcccgggtc
    11401 aggccgatcc ggctcttgaa gtactcaaaa cccgattctg ccaccgctaa tgtggcactt
    11461 gtcaaaataa cgcgcggaat ttcctgaaac aggtgctcgc gcaaaattgg accgacatca
    11521 atgggagcgg ctccgagaat aatgcggggg acttgacggg cctcctggac ttctacccag
    11581 tacacgtagc cctcgtggga ttgccggatc cactgatcaa cctgagtggc taacgattgc
    11641 agacggtccc gcacggccac caagtcttga cgttcctcct ctttttcgat ccgcgctgct
    11701 tccttgtgaa cgatcgaggc gagcttctgc agaccttctg tcagcgtatt gcgaaagacg
    11761 tccctttgcc gcacccgccc attgccgggc ttctgctttt cgtaccagtc aaacacctgc
    11821 gcgaaaaact cgtcgcaccg atagagacag tccatgacag ccttttgtcc ctccgggctt
    11881 cgataatgaa cgaaaagccc gcgttgcgtc cgctcgttgt agagcttacg cagattgtac
    11941 atgatctgcg tgttgcttac ttctgtcccg aattgttccg aagccaccga ctccaatgtg
    12001 tgggcttcgt caatgaccag caggtcgtac tcgggaagga tgtttgctcc ttctctacgg
    12061 agtgcaaggt caacacacag cagggcgtga ttgacgatga ggatttgcgc ccgttgtgcc
    12121 cgccgccgcg ccgcaaaata aaagcattga ttgtaatgcg gacagcggct tcccatgcaa
    12181 ttgtccttgt cgctagcaat ttcgtcccag accgtggcga agggccgaaa cgaaagttcc
    12241 gagagcgttc catccgtcgt ttgggctgcc cattgttgga tggcctgaag ctgctcccat
    12301 tcctcgtctg cgaagagcgt ccgcgacctc cttaaagccg tggctaaccg ccggaggctg
    12361 atgtaatttc cccgtccttt gaccagcacg gcggtgaact catacggcac gacactccgc
    12421 aggaacggaa tatctttgtt aagaagctgc tcctgaaggc tgattgtgtg ggttgaaatg
    12481 acagcccgct ggatatcgtg tgcatccaac tcgccagcga cagccaggat cgtcggaacg
    12541 aggtaggcaa aactttttcc cacgcctgtc ccggcctctg caatcagcgg acgctggctg
    12601 gtgatggcgc gctccacggc ctcggccatc tggagctgct ggggccggtg ctcatagcca
    12661 ggtaagcgga cggcgatccg accttcaggt cctagaatat ctttcgttcg caccatgtcg
    12721 acgatgtttc cagtataact tcacaaatgc cgtgttcagg aaggtcagtg gcagtatctt
    12781 cgaggaagtt gcacggccct tccctttaag ttatcctaaa gcagactatt cggccattgc
    12841 agaagaactt aaccttcacg aaacgctcaa gatttgggga gttcttgcga aagagaatta
    12901 ggagggcctg gtccggctcg gccaggacgc acaacaagca aacgagatct agcgtggcaa
    12961 tcgagttcta ttgctctcag tgtcgaaatc gcctccgcgt cgatgactcg gcagcagggc
    13021 ggcaagcccg gtgtccggtt tgcaacgcca ttatgattgt gccggctgtc ggtgtcattc
    13081 aagagccgcc cggtgcagtg cggagcgaat tcgaaggtta tattcccagc gtaggtccga
    13141 cgcgggtttc accaccaccg cccggaacga tgggcggcac cacgctaggc tctggcagac
    13201 ctggtccaga gccggctaca taccaaccct atgtcacgcc tcagcattat ggcgaagtcg
    13261 tggggcgatc ggccgagaag cgcgaggaag ggtggggagg tgcggctttg gtccttgggt
    13321 tatttggact tattgcctgg tgctgtccca ttatcggtgt ggtcataggt ggcctcgccc
    13381 tggtttttgg aattgtctcc cttggcaaag gcggcgggcc tatggcaatt atcggtctta
    13441 ttctgggcat catctgtttg gttttgagta ttttcaacgc gctcctaggc atggcgctga
    13501 tgctggccga tgctgaggtg gtgtcgctgg taatgaccga tctgtgatcg aaacgtatgt
    13561 cctttgtttc ttacagaagg cgacaatatc cgtaataaga gtccatccca gaacgtcccc
    13621 ctctcccgcc gggagagggt tggggtgagg gcaagcgatg gtcggaatcg atcgagaaaa
    13681 ccggcagcga ctcgagccac cctcaccccc gacccctctc ccgccaaacg ggcgagggga
    13741 gactatagga taggctctaa atagcagggg aacctcaaaa tgcctattac gtttcagtgc
    13801 cccgcgtgtt atgcgatgct taaagtaccc gatgacaccg ttggaaaaca ggccaaatgc
    13861 ccccgctgcg gccagattgt gacgatttct gctggtgcgc cagccgcacc aagttctgat
    13921 atgggcgctg ccgtcccgcc acctatcggg ggagaaccac ctcgtcctca agagcccaca
    13981 agcgttaatc cgtatgctcc accacaggcc caaacttctt atctggcacc gatagaccgc
    14041 gggaccattg agccacaaat tatctctttt agcgaaacat tcaacgtaac gtggcgactg
    14101 ttcttcgata acatcgggcc atttctcggg atgggggcac tgcttttcgg gctaactttt
    14161 gtagggaata tgttcgtcaa ctttatcagt ttgtttttcg cggcagcggg agacgatgaa
    14221 gcgatgatct ttcttggcat catcgtactc atcttttccg tgctcatggc cgcggtaagc
    14281 atgtggttac aaggcggcat gctccgctac agcgtcgagc ttgccaaagg gatgaggccc
    14341 aactttgggc ggattttcct cccagtcaac caattcctgg gatatttcgc agtcgtaatt
    14401 ttgttgtcgc ttatagttac tgccggtttt attctgctga tcttaccggg aattatccta
    14461 atgctcatgc ttttttgtgc cccggccgtc tatctggatg gcaaggctgg ggtcatcgag
    14521 tcattttccg tatccagaac cattacccgg ggaaatcgtc ttacgatttt cggcctcgtc
    14581 ttggtcaata cgatccttgc ctttctggcg accgtgctga cgtgtggact tgccggtatt
    14641 cttgctcctg cgttcttttc gttattgacg gcggtcattt acacgcgatc tgcagggcta
    14701 ttcgcatcag cacgtccccc gatggtccag atgccgcctc cgagttggat gccggggccg
    14761 agtccagggc ttcgctagcg atttctccaa tcgtttcggg agaagagcgt ggggaagaag
    14821 atcagcgata acggcaaaca gcgcaagagg atgcgctgtt gcgtgattct ccttagttgc
    14881 cagtcctctc ggtgctgtca gccaccgggg agcgtaaatc ccaggcattg agccagcggg
    14941 tcttttccac cgcaaagcca atctgtgtca ggcgatcgtg gaaatggggc atatggccgg
    15001 ccccgtaaaa aatcgcgacg gattttgcgc cctttttcgc ttgatcctcc aacacggcca
    15061 agacccgctc gtttcgcccg ctaatgagtg tggacccctg gggaccttcc aatgcccgaa
    15121 tttgaccttc tacatcctcg gataattgct gcgcgaaaag tcgcttcagt tgaagttggc
    15181 ggtctttgct aaacaaagca cgtaccaagt cggcatcatt cggcgtgctt tgtcttgaaa
    15241 gactccttgc ccattcgtaa ccgaaggccc gaagcagcat cgcccaggtg ctttcgcctt
    15301 tttccttcat ggattgataa aactcttccg gcgagagatc ggcatgaaca aaattggggc
    15361 gatggtaatc gatcacgtca agctgatagg ccaggcccaa catatttgtc agagattgtt
    15421 ggagtgccga gaccgggtgg gccggccgtt gtcctggtgt aacccgtgtc ccttccggcg
    15481 caaccagttc gtagaggacg acatcgtact tgcgaagaat cttgttgagt tgttcatagt
    15541 agcctctgtc tgcgatgtgg atgaccccaa ccagatccac cctcaggtct ttaggccagg
    15601 caggttgcga gcgacctgtt tctcccgcgg aaagtgtttt gccttgtgat gaatcatctc
    15661 gaaccacata actgacgatg gctgtttgaa gatactgagg ttcgtttttc cgcacgagca
    15721 gccggacgaa ttgaggccga ttggccttct gttccccgga ggaggttttc cctgccgact
    15781 cgtcgacccc ctccgcggtt tggggtcgct gcttctttcc gtgaccctgt gacctcccgg
    15841 aaggagacgt ggagggttgc tcgacttttg ttgcgtctgc tttgccttcg aacagcacca
    15901 gcggcgcgac gtagtctgga ctcgcctgtt gggctgcacc tagtaacgcg cccggggaga
    15961 aaatgaggta ggcgaagagc acgggcgtga gcaacccgct aaagacaaac agataacgaa
    16021 gcccaagatg gggaacccga tggctcatat tcgttccctc taccatgcac gagtttnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggt acagggacca gcagtttggg
    16201 tcagggcgcc cttttgtaat ccggttttca aacgctcatg agatccacaa acggggtggt
    16261 taaagggagc gcttggccgg atgtctctta ttttggaccc ggtcatggca agagaagctt
    16321 cggttaatgt cccatcccgc gtatggacca cgccgatcgt ggcgttgact tgacgcttta
    16381 ggatgccgta atcatgataa aacagtcgag atggctattt tagcaaagat atgcaaggtg
    16441 gccgccagtt cctattgccg ggtgatgatc gcttcaaagg aggccgtgct tttctactca
    16501 aagaggagtt gggtgatccg gtgggaacgt catatgggtg gtcttacgga gtgactgggc
    16561 acaccacagt agggggtaat ggggtggcga tcaagaggcg agtgtgaaca ccaagtcagc
    16621 actttttgac ctttggcaga ctaacggttg aggacatctc actatgttga aacagcgggt
    16681 tgctcgtcag cgaaggaagg gttctatgct tgccaacctg tgggcttcgt tgcgtcaggc
    16741 tcgaaacaaa tctcattcct cacgggagcg gcgacgcctg cgtgttgacg ccttggaaga
    16801 gcgtacgctt ctcagcgtga cgcccgtgga tattctcgac aagcttgtga accagtcgct
    16861 tatcccgtcg ggagggacaa ttgctgggca atcgctggcg gtggacgctg atggcgattt
    16921 tgtcgtcgtt tggacgcgct atgacacgct gaccgacccc aacaccggtc ttcccatcat
    16981 tgaccccctg acgggccaac cccggaccga ggccaacatc tatgcccggt atttgacgaa
    17041 tgaagttcag cggattaccc ttcccagcgg cgtgttaaac gacacggtgc ccacccaata
    17101 cggccgattc tccctcattt acggtggcac gacccttgtt caaaaattga ccatttcggc
    17161 cacttatgag ccatacgtta ccactcaaca gaatatcgcc ggtacgtttg ttctccaggc
    17221 tgacgcaaat ggcgatggga tcatcgcagc gacggagagg gcgaccatcg tttatgacga
    17281 aaccacggat gtcaatacgc tagccagcac aattcaaacg gcccttcagg ggatcggtgg
    17341 acctctccaa aatgcgcggg tgagcgcagt caatgccaag gagtttgtca tcgagtatgg
    17401 cagtgccgcg cttggcatta accaaccgaa gctttcggtg gggaatgtca actttacgag
    17461 tgggttttat cccagcgtgg aagtgagcac ggcgcgggag ccgatcctga tttccaacat
    17521 tcctgtttcg cccgacaatc cggctttgac ggcgcaggca cttgcccagg cgttcaatac
    17581 gcggcttcag gcttatgccc ttggaccgat tgattttcca ccgcccgatc gtgtgcccag
    17641 cgctaccgaa gggccctaca ctgacccact tgtggccgct gtgagcattc cgcagatctc
    17701 cgtgaccgcg gtgaatgcca ccacgtttga tattcgattc atcggagatt ccggcaagct
    17761 agatcaccca gagctgatta tcagtgctgt gagcgacgag ctaggtaata acctcctgcc
    17821 ttctccagag gctaccgtca ccaccctgaa gcagtcgagc cctgagttcc gggtgaatcc
    17881 cgaggagccg gatgatcctt tcacaccagg accggacaag tataaccaaa ccaatccggc
    17941 cgtggccatt gatgccgatg gcgacttcat catcgtgtgg gagagcgaag ttcccgattc
    18001 cgtggccttt gccagcgtct cggacatttt tgcccgccgt ttccgtccgg tcgggacggc
    18061 caatttgggc gtctcgcagt tcattgtgga tatggacgcg gatggcactc ccgaaacacc
    18121 catcagcggt attttgccgc ttgtttcacc tcttaagacc gtgacccaga cgttgacggt
    18181 tgatgccttt ttgccgggca ccttgacagg aaacttccgt ttgagtttgg caggtcgcca
    18241 aacggaggac attgcgttcg atagcaccca gttggctacc acggcggcca acatccaggg
    18301 tgccttggtg atggccggtt tccaggatgt cacggttcag gtcatttcct ccactgatcc
    18361 ctacaagttc cgcgtgacat tccggcagac cttctctggt cccaatggcc tggccgacgc
    18421 cattctgcaa tatgtagatc catctgcagg ggtgcctctt tcgacacggg ccagcgtgtc
    18481 atcacaactg gctccgggat cggaccttta taccttccag gtgaatacgg agacgctgcg
    18541 tgcccagttt gccccggcag tggcaatgga catgctcggc aactttgtca ttgcgtgggc
    18601 caatagcgcg caagacttca gttactacaa cggtattaaa gcccaaatct ttgatcgcaa
    18661 cggtaacaaa atcggagagg agttcctggt caacaccgag gacacggctg tttacctcaa
    18721 tccagcgata ggaatggccc gtacaggtga gttcgtcatc gcctgggagc gaactcacga
    18781 tgtcaactac ctgacgggtg gggcctatgc aacagcggtc caggccaagg catataacct
    18841 tgctggccag gtgattctcc cacaattcgg cgtgggcggc ggtggtgatc ctgctattgc
    18901 tttcgactcg gccgatcatt tccttatcac ctggcaagtg gtgggcgatc cggacaacac
    18961 gggtcagaca acctcaggtg tccgcggaat tgtgtatgac ctgagcggaa acaccattcg
    19021 ggacgtcttc cgggcgaaca gtgctacgtt cgatcctgcg gacgacacat tgtggcctct
    19081 ttatcagggt ggggcccagg gcggattgga tgccgacggc gatatgtttg tcgtatatga
    19141 cggctatgga cccgatgtgt cgcaaaatgt ctatggattg acgacggccg tccagcaatt
    19201 attgagaagc cggattaacg ccaccacgaa tgctgatctc ctcaactact ttgatcctca
    19261 aaacgagtct ttgctgggtg ccggattacc aagcaatggt gacgtcgatg gagtcataga
    19321 aaccattctg attcgtgcgc tgaaccgcgg ggccaatgcg gaacagatcg gacggttgcg
    19381 ggcgattctc gacagcgtcg caggactgtt gcgcggtgaa gccaacggcg tcatggtcag
    19441 ccgattcgac gccagtccca cgatggccta cacgacgctg ttcagcgata gcgtggtcaa
    19501 cacgctccgt gatggccata acactcggta tctcattctc atgaatcagg cgctaaattg
    19561 ggaatccttt atcatccgct tgtaccgcga tggatttggc ggctacgaag acgttaccat
    19621 caacaacatc caaaccaatc agaactggaa cattacgctc acgcgggatc gaattgacga
    19681 attgctggag ggtgcagcac gcaccggaac caactgggct gagacccgct acgaggggtc
    19741 cattgatgtc cgcatcttgt cggcctttga agttgccagc cgtatcggaa cgccatggga
    19801 aataccggtt gccaatccga atcagtacgt gatctacgaa gtcaccttcc agggtgaagg
    19861 tcacgattgg ccgttttcta tggtgggtgt ttggccaggg cgtgctggcg gaaatgatgt
    19921 tcccccacca ttgatgatcg tcgagaccta cggcgatcgt ggtgtgacgc aagcggatgc
    19981 gtccatgggg gtcacgccgg agggttcgtt tgtcgttatc tatacgcaat acgagcagaa
    20041 taccgatggg acggtgacaa acgctacgat tcgttaccgc caattccagg aaacagcaga
    20101 caccgccggg ccgttcgtga ctgacatcct ggctcccacc ggcacaagcc tgtggggcgg
    20161 tgccacggtg caaggacctg ttaaccagat tgtggtgacg tttaatgaag agctgttagg
    20221 cggcgatcca caatccgttt tggatagcgt ccttaatcct gagaactttg tcctctatcg
    20281 taacggcgtt gagattcctc gagggatcgt gaaagtcgat ttcggcatga acgccgcgtc
    20341 gctcctcaac ggaaaggctt atgatctcga tggcgacggt gtgcccgatg gaacgtatca
    20401 gctttatgcc ctcccgacga acaagtggga ggcggtcctc accgtagatg gcaacggcgc
    20461 cttggaagcc ggaacgcctg gattagggga tggggattac caaatcgtgt tgcgaacgcc
    20521 ctcgggggcg acctcaggcc ttcgcgacaa ggccggtaac ccgctgaatt caacgggttt
    20581 cctccctgct ggcgccaata cctcgttgag tttccatgtt acggtatcag cccaggatca
    20641 gcccgtgctt gatctcaatg ctcggacgca gccggagagt ccgaacgcgg tggcacggga
    20701 tgctgatggg gactatgtgg tggtttggac gcagacggac gcgagtgggc ggaatcgggt
    20761 gtatttccgg ctgtatgatc gggatgggac gccggcggac ttggatgtga acgggaatgg
    20821 ggtgatagat ccgtttgaga ttgacaatgc tccggcgacg ccggtgacga cgagttcgac
    20881 gtttgcgggt gacgatcagc agtatgcgtc ggtggcgatg acgccggatg gggattttgt
    20941 gattacgtgg acgaacacac ggagtgggaa tgccgacatt tatgcgcgtc ggttttcggc
    21001 gaatggtcag gcgttgggcg aggcgttctt ggtgaacacg gtcacggcga acgaccagaa
    21061 gtggtccgat gtggccatgg acgccgaggg caattttgtg attgtgtggt caagttgggg
    21121 ccaagagccg ggtggtgtgg ggaatcgttg gggcattttc gcgcggcgtt atgaccaatt
    21181 gggccagcca gtcggtttgg aatttcaggt gaacacgatc gtggctggtg atcaggtgtt
    21241 gccgagcgta tcgatggact atcggggcaa ttttgcggtg gcttggcaga cggcgggtga
    21301 gattgcgttg cgaacgtttg acgcaacggg ggtagcccgg gaggcagcgg agatactgct
    21361 aggccagacc attgttggcc agaaggcata tccggacgtg gccttggcgg catcggggca
    21421 gtatgtagta gtgagttggg ctgggccgga tggcaacggc aacggggtgt atgtggactt
    21481 gttgcaatgg gaccctgcgg ggcttgtgtg gacgccggta gggacgagcc agttggtcaa
    21541 caccacggtg gttggtgagc agagctatgc ttctgtggcc atggctgtga acgggagctt
    21601 tgtggtctcc tggagcggta ttgggacgca ggtgggtcaa gaggatcagt tagagtcggg
    21661 cgttttctat cagcaattct ccaacgcgct gaccaaggtc ggtggcgaac gtcgagtgaa
    21721 ccaagtggta gctggaaagc agtgggcgcc gtcggtgggg gcggatgcgg acgggaattt
    21781 catcgtggta tttacgggtc cgtcggcggt gaatccggcg ttgacgacgg tgtatcggtt
    21841 tgacagccgg gtgtatttga atcagggcga cgtatcggcg cccattgtga cgaaggtctt
    21901 gacgggcgag ggggtgttgg tgacggaggg ggcgcgggtg gcaccaggtc cgcaggagtt
    21961 agtggtggtg ctaagtgagg cgatgtccga gcggttggcg gacatcaatg gtgatggact
    22021 aatcgacaat cgggatggtc cggcgccgga tagcgtgttg aatgtgcaga attggaagtt
    22081 gtatcgggat ggggcggagg tagcgggggc gatcaaatcg gtgacgttcc ggtacaacgc
    22141 gttgacacgg cgatacgagg cggtgttggg gctggacagc aatccggtga cgacggcgat
    22201 cgagccattg gtaccgggtg agtaccaatt ggtggtgaag gatgtgatga cggacgcgta
    22261 tccgtacttc cggttgacgg atccggtgac aggtcgtcag ttggatgggg accgtgatgg
    22321 tgtggccggg acggcaggtg gggcggctgg atttgtgcgg aatttcatgg tgattggcgg
    22381 ggtacctggt caggatgtgt cggtgattcc tggggtaccg acgcagaatg ctcggacgca
    22441 gccggagagt ccgaacgcgg tggcacggga tgctgatggg gactatgtgg tggtttggac
    22501 gcagacggac gcgagtgggc ggaatcgggt gtatttccgg ctgtatgatc gggatgggac
    22561 gccggcggac ttggatgtga acgggaatgg ggtgatagat ccgtttgaga ttgacaatgc
    22621 tccggcgacg ccggtgacga cgagttcgac gtttgcgggt gacgatcagc agtatgcgtc
    22681 ggtggcgatg acgccggatg gggattttgt gattacgtgg acgaacacac ggagtgggaa
    22741 tgccgacatt tatgcgcgtc ggttttcggc gaatggtcag gcgttgggcg aggcgttctt
    22801 ggtgaacacg gtcacggcga acgaccagaa gtggtccgat gtggccatgg acgccgaggg
    22861 caattttgtg attgtgtggt caagttgggg ccaagagccg ggtggtgtgg ggaatcgttg
    22921 gggcattttc gcgcggcgtt atgaccaatt gggccagcca gtcggtttgg aatttcaggt
    22981 gaacacgatc gtggctggtg atcaggtgtt gccgagcgta tcgatggact atcggggcaa
    23041 ttttgcggtg gcttggcaga cggcgggtga gattgcgttg cgaacgtttg acgcaacggg
    23101 ggtagcccgg gaggcagcgg agatactgct aggccagacc attgttggcc agaaggcata
    23161 tccggacgtg gccttggcgg catcggggca gtatgtagta gtgagttggg ctgggccgga
    23221 tggcaacggc aacggggtgt atgtggactt gttgcaatgg gaccctgcgg ggcttgtgtg
    23281 gacgccggta gggacgagcc agttggtcaa caccacggtg gttggtgagc agagctatgc
    23341 ttctgtggcc atggctgtga acgggagctt tgtggtctcc tggagcggta ttgggacgca
    23401 ggtgggtcaa gaggatcagt tagagtcggg cgttttctat cagcaattct ccaacgcgct
    23461 gaccaaggtc ggtggcgaac gtcgagtgaa ccaagtggta gctggaaagc agtgggcgcc
    23521 gtcggtgggg gcggatgcgg acgggaattt catcgtggta tttacgggtc cgtcggcggt
    23581 gaatccggcg ttgacgacgg tgtatcggtt tgacagccgg gtgtatttga atcagggcga
    23641 cgtatcggcg cccattgtga cgaaggtctt gacgggcgag ggggtgttgg tgacggaggg
    23701 ggcgcgggtg gcaccaggtc cgcaggagtt agtggtggtg ctaagtgagg cgatgtccga
    23761 gcggttggcg gacatcaatg gtgatggact aatcgacaat cgggatggtc cggcgccgga
    23821 tagcgtgttg aatgtgcaga attggaagtt gtatcgggat ggggcggagg tagcgggggc
    23881 gatcaaatcg gtgacgttcc ggtacaacgc gttgacacgg cgatacgagg cggtgttggg
    23941 gctggacagc aatccggtga cgacggcgat cgagccattg gtaccgggtg agtaccaatt
    24001 ggtggtgaag gatgtgatga cggacgcgta tccgtacttc cggttgacgg atccggtgac
    24061 aggtcgtcag ttggatgggg accgtgatgg tgtggccggg acggcaggtg gggcggctgg
    24121 atttgtgcgg aatttccggg tggtatcccc accatcgccg cctgcccctg atgttccagt
    24181 tgttccagtc gcaggtgacg caattctgcg cacccagccg gagagtccga acgcggtcgc
    24241 ccgggatgcc gatggcgact acgtcgtggt ttggacgcag accgacgcca gcgggcggaa
    24301 tcgggtgtac ttccggctgt tcgaccggga tggcaccccg gcagacttgg atctcaacgg
    24361 taacggtgtg attgacgcct ccgaagtcaa taacgcccca gcgacaccgg tcacgaccag
    24421 ttccaccttc gcgacggacg atcagcagta tgcctcggtg gcgatgacac cggacgggga
    24481 cttcgtcatc acctggacca acacccgcag cggaaatgcc gacatttacg cccgacggtt
    24541 tgcggccagc ggccaacctc tgggcgaagc cttcttggtg aacactttca cagtcaacga
    24601 ccaaaagtgg tcggatgtgg ctacggacgc cgagggcaac tttattgtgg tttggtctag
    24661 ctacaatcag ttggatatcc ctggtgcgca aaacacgtgg gacattttcg gaaggcgatt
    24721 tgatcgtttt ggacaaccgt tgggcgtgga atttctcgtc aatgtgacga cgacgggtga
    24781 ccaaaccctt cccagtgttg caatggacat tcaaggtgga ttcgtagttg cttggcaatg
    24841 caatcccgga cgggaaggaa aggatgttta tgcacgttcg ttctggcccg acggatcccc
    24901 tcaagcggag ggtgtgggtg caggatggta ctacggcgag ttccttgtga accagacact
    24961 tgccggagac caggtctctc ccgacgtagc aatgtcacca tctggcgacc aattcgtggt
    25021 tacatggaca ggaccagact cagattcttc tggagtgtat ttccgccaat ttagtagaac
    25081 tgttgatatt tttgcaccat tgcggcgggt atataactcc aacatttcgg ctcctggact
    25141 gcgccttgga cacccggtaa tatcaactgg tccgtataca gttcaagttc catacaccgt
    25201 tgtagattca attactatta atgatgtggc gcaaatccaa gacctcaacg taacaattaa
    25261 tatccagcac cgacgggcaa gcgacttgtt gattgagctt cggcacgtcg acacaaatac
    25321 cgagatcgtc ctttcgaacc gcaatcctca gcgacccgcc gggacttacg cttccggagc
    25381 gaattacatc aacaccactt ttgacgacga ggctgcaacg actatcgtca ccggcagccc
    25441 accctttacc ggaagttttg ttcccatgcc cgacgccctt tcagcgttcg atggtcaaag
    25501 cttagcgggc acatgggaac tcgtcattac tgacatgtat tatgattaca acaatgatcc
    25561 acgagattgg cctttcgtgc tcgatggtta tctcatctca tggcagctaa gctttgctac
    25621 ggtacctccg tcgtctgcgg aaatccttgt caacgaaacc acggccggtc ctcagttcgc
    25681 tccctcgatt gctatggcat acgacggaag ttttgtgatt acttggacag ggacggggct
    25741 agttccaagt caggaagaca tttctgggac aggcgtcttt taccgaaagt ttgacgcttt
    25801 ggcagccccc accacgggtg agacgcgggc taacaacacg atccaaggcg atcaacgctt
    25861 ttcctctgtt gcaatggacg cggacggcaa tttcataatt gtgttcactg ggccagtggc
    25921 aagcgcgcct ggattgacca ctgtgttcca gtttgatagc cgagtccatc ttttggcaaa
    25981 cgaattgata ccgttcgaac cggactcggc agctcctttg gttaccagcg ttcagacaaa
    26041 tgatggacga ccgatataca ccaatgccat tcttttgcag ccagtcaccc aaatcgtggt
    26101 ggctttcagc gagaatctca acaccgatct tttggatact gatggagact acgtcttaga
    26161 tacgcctggg ctcagctcaa tcctcaaccc caaaaacttt gaattgatca aggatggtca
    26221 atccttgccg ttttcggtta ttgcggttga ctttggttgg aatgcggtca gccgcaaata
    26281 cgaggcgaga ctcacgctcg acggggatac cgtcatgccg ggtgtgcaag cactcggaaa
    26341 aggggtctac gctctgcgaa ttagcgatgc cgtggtcgac ctcgtgaacc accgcttgga
    26401 cgccgataga gatggaacac ccggcgacgc ttctggtcaa ggatactatc agattgattt
    26461 cgcagttggt cccctgtcgc agacttatga acatctggtc aaccagtcgg tcgagtatgt
    26521 acaagctttt gcagtatctc agggaccagg actcgcggtg gatcgaaatc gcaaaacgct
    26581 tgccgtcgat gctgagggaa actttgctgt tgtttggatg agtttcgggc aggatgatcg
    26641 gagcgattca actggagcgg gcgtttatgt gcgggtgttt gaccgggccg gggtgccgat
    26701 aacgccggaa atccttgtca accagacggt tgtcggaagc caaacgaatc ccgtgatcgc
    26761 aatggacgcg gacggtgact tcgttgtggt gtgggaaagc caagataacg tagtcgacgg
    26821 aagccgtggg atttgggcaa gactcttcaa ctccgtggga cagccactta cggatgagat
    26881 tctagtgaat acccttaccg caggcgacca gtacagccca tcggtggcaa tggatgactt
    26941 cggcaacttc gccgtggttt gggcaacgcg tgctcaaccc ttcagctact ataacgacat
    27001 taaggggcaa cttttcagct accgaggtga aaagatcgga tccgagtttt tggtcaacga
    27061 gcaaaatatt gctgggacaa ctcttctgcc agggagtaac gaagttcatc ccgctgtggc
    27121 aatggatccc cagggcaact tcgtcgtggc ttgggaacag gtggtccagc aacggaatgg
    27181 tgttggtttg gacactgtta tcgtcggaag actgttccaa tcaaatggag cgccgcttac
    27241 tggtgaattt gcgatcagcc aaggtgacac cggtttggta gatcccgagc atgataccga
    27301 cgacgcatct ggtggtgctg atatccaacg tcgcgctcgg aacccgcaag ttatcatgga
    27361 ccgtttggga aacttcagcg tggtttggga ggcttatcag gataatgaca ttggccagca
    27421 gggtgccgat agttatggca tatacttccg ccgatttaac tccgatgcca cgccagcaga
    27481 tgatgaacat caggccaatc tcgtggttac agaaaccacg caaggagcgg tcgcacctgg
    27541 tattccaagc cgttatttcg cttataacca attgaaccca tcgattgcaa tggacgctaa
    27601 cgggcaatat gtggttgtct gggatggcaa cggttcggct ccccacgttc tctcagtcac
    27661 ggataagttg tacaccgatt gggaccgaga tggtgtgtgg atccgccaat tccaggcaac
    27721 gactgccgga ggtgatttgg aatttgttag ccctcagact cgcgtaccgt acacaagtta
    27781 cgggccgcag cagtttccgt ccgtcgtgaa aactttggac ggggatatca tcgtggtgtg
    27841 gagcggacgc ggaatcggag acccgaacgg aattttcttc cgaaaataca cttcaatcga
    27901 cgctgctggc ccgattgtga ccgacgtcta cgcgggtgaa gggattcgtc ttgtaggttc
    27961 gcgatctgga aatgtgcgcg tcagccaacc ggttaataca ttcaccgttg tattcagtga
    28021 agacatgatg cgtaccggtc cagactcggt gaccaatcct cagaattacc tattgatcaa
    28081 agacggctcc gaaatcgttg ccattaagag cgtaacattt ggccttaatc cgcagaccaa
    28141 caaatgggaa gcggtattaa ccgttgacag cgactcacta gccccgggtg atcagccttt
    28201 acccagtggc aactatgagt tgcgagtttt gtcgcctgtt ctcgccgcgg gaggcacgcc
    28261 gggacggagt ggagtacgag acaaagtggg caacgcgctc aatgaatccg gactgagtca
    28321 gggcggtcaa aacttcacgt tgaatttcca ggtcgttgtg gtcggcacca gccctggaac
    28381 accgggaacc ggcgcaacgg actttgccgt caatactacg caggctgatg accaaacaga
    28441 gccagtggta gcaattgatt ccagaactgg caatgtaatc gtcgtgtgga ctagccgaag
    28501 caccgaacag tatccggatg gaagcacggc actcgccacc gatattttat gccagatcta
    28561 caattcattc ggcgagccca tcgggccaga gtttgtcgta aacaacgaca caattggcaa
    28621 ccaatttcaa cctgccgttg ccgttgatgg tttaggtcgg ttcatcgtaa catgggctgg
    28681 cgaggggcag tctgacgcgg atggcggcat ttgggcacgc attttcgatt cccaaggcag
    28741 tgcgctggga cagcagttcc gggttaatca attccggcaa tacactcagc acagccccaa
    28801 ggtagctacc gattccgcag ggaacttcgt tatcacctgg agtagctatg gacagtcgga
    28861 cgatattgac gctgtttacg cccggcttta cacccagtcc ggacaggcca aggggaatga
    28921 gttccgcctc aacaccacca cggccggtta ccagcggaac tccgatgtgg ccatggatga
    28981 cgctggcaac ttcgtcgccg tctggatgag cgacggacag gacggtagcg cccatggagt
    29041 tgtgggacgc gttttcaatg ccaatggcac ggcgcgaacg ggcgagttcc tcgtcaatca
    29101 atatacgccg aacgagcagc gcgaccctcg ggtggccatg aatggagcag gagatttcgt
    29161 ggtaacctgg acaagttatg ggcaggatgg tagcggctac ggagtgtatg cccgatgcta
    29221 cggttccaat ggcgttgcca agggaaacga gttccgggtc aatcagacca cgctgtactg
    29281 gcaatttcag cccgatgtgg ccatggacgc ccagggcaac tttaccatcg tgtggactac
    29341 gacgggacaa gacaacgacc ttgccaatga caatggcatc tacgcccgga tgttcgcagc
    29401 cgacggttcg gaccgttcga tccccaactt ggggaaactg ggagaattcc gcgtcaatgc
    29461 ctttgtgact ggcaaccaga acacaccagc gatcggtatg gacagggctg gcaactttgt
    29521 cgtggcctgg tcgggtccct cgacagaccc tgatcccatc gtggctgcca cgaacgggac
    29581 agacgttttt gcccgctggt tccagctccc atctctcctc cgaatgtcgc aaaccgcgaa
    29641 cgagttggtc atcgaaggca ccaacggcaa cgatgtgttc gagttcactg ccggtacgtc
    29701 cttgaccaaa tcgaccttga aagtgaatgg tcaaaccgtt tctattccgt caggtgtttc
    29761 gcgggtcgta tttgatgcca agggtgggca ggacaaaatt accatcacag gcaccagtgg
    29821 tgccgacact gcgacgtttt cacccaccca gactaccttc gagttcggct cttcccagac
    29881 gctggtggtg ctcaatgggg aacaggtgaa gattgacggc aaagggggaa cggatgccct
    29941 gcgggtttac gactcgccac gcacggacaa ttacaccgct acgccgaccc gcgtcacggg
    30001 gagcgataat ctgagttcgt actctttgga ggcgacgaat ttcgaaaacg tggtgcttac
    30061 gtcacgtgct gggggtgagg acgtagccat gctctacgac tcacccggcg acgatacctt
    30121 tacggcgaca cccacgtacg gcgaaatggt tggcacaggc tacaagctgc gggcggaagg
    30181 tttcgccttc ctgcacggct atgcctcggc aggtgggact gataccgcca tgctttacga
    30241 ttcccctggt gacgacgagt acgtctgcac cccggattac aacatcttga gaggtgagaa
    30301 cttctacctc cgggcgaagt atttcgatta cgtccatgct tacgccactg ccggcggata
    30361 tgacaaagcc accattaagg gctccgctgg gaatgacaag attaacctgg accagaaggt
    30421 ggttcggctc aattccggct cttacgtcca tcgtggcaag ttcttcgagg aggtgaccgt
    30481 ccgtggttgc ggtgggcaag acaccgccta tgtgactgac ggcatcgttc taccaggaac
    30541 cgtcagccgt ggcaccccgc cgatttccct ggacgcctcc cagattgtct atctccggca
    30601 gctttacaaa atccagacga ccaatgaggg aggtaatggt accaatcgca cgatccgtcc
    30661 cgccgcggac gccgtgttta cggcctactg gccgtgattt gaaacgtcct ggaagggcgg
    30721 gaacctttcg ccccttaaga tccgggaacc atagcacggt cgcacttgat gacagaccaa
    30781 aagctttcga agcccaccat tatggtgggc ttttttgttg gtcaaccttg agtttttatt
    30841 ggtatgatca tggcagatgt accccatccc acgtaggttg cccggtcttg gccgagtccc
    30901 ggcgtagcga agccgcgatt ttcacccacc ggtaggggca attcatgaat tgcccctacc
    30961 atcctgagac agcgacccct tcaacatggc tgtaaaactg gttggttgtg aagatggggt
    31021 atacgcggag tgtgtacaat ctaggtgtgc taaaagagga ttgtaagcgt ggataatgtg
    31081 ggcagaacaa acggatggaa ggggattttt cgaagtgccg ctccgaacag cgacgaatct
    31141 aggaaatact gacgccgttg gattgcttgg tattttcgtt aagttttgcc attctttttc
    31201 aactctcaag cacgagtttt ccgatacaat cctcgcaacc ccatgttgcc ggttctccac
    31261 gaggagtgat tgtggcgagg acggaacatt tgcaaacatt gaggtggacc aggactatgt
    31321 actgccaacg caaagcgatg cggacgattg tcggggtttt tcttctggct cttgcaaccc
    31381 cagccttggc ccaggttaaa gacatgcagt atttcgcacc ggctcaggtc gaaaacttcg
    31441 gaggaggccg gcgagccaac gagggcttct ttatcagcgg ggaatacctc cggtggagca
    31501 tttcgaagcc ggatgtaaca tcggtcggtt atccagggtt aacccgccag gtgtactacg
    31561 ataccgacat ctcacgggag cagacaaaca cccttgacac gagtttcatc ggggccaaat
    31621 tcaccgatgg aagtcgtgtg gaccttggct acatcgaggg ccacgaggga tggcttttca
    31681 gcgggacatt cctttcaaca cttggtcgaa cccatggtgt ggccagtgcc gacatcgtat
    31741 tcgccgataa ggagttcggg gacccgccgc gctattactt acaaggttac gttgatgatg
    31801 cgctaacaaa tattcagaat ctgcccgtca ggttcaccgc accctacatc gaggacattg
    31861 aagcggacga aggcggtgat cccgattccc tgcccgagcc ctcttggggc gtgatgatgg
    31921 aaaacctcac cgagcactgg agcacagagt tgaactacat ataccgttgc caccccaccc
    31981 ggcggggcgc aacctgggaa ctattcctcg gtgtgcgata cttcgagcta aatgaagact
    32041 tcaatgtgtg ggcgccttac tcctatgtcc cgtacgatcc aactgcggat gaaaacgacc
    32101 cagctggtgt cggattccca acgcgtgtgg gtatcttggc tgatagttac tggttcacaa
    32161 aggccgaaa
//
LOCUS       DAOSUT010000025        32001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_968_length_32001_cov_9.349997, whole genome shotgun sequence.
ACCESSION   DAOSUT010000025 DAOSUT010000000
VERSION     DAOSUT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 32001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32001
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_32001_cov_9.349997"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1941
                     /locus_tag="PLD05_03675"
     CDS             55..1941
                     /locus_tag="PLD05_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOP76560.1"
                     /translation="MTQFRYLVPLHQAQDWIRDGDLLLFRRRGLIAAAGRGEHSHAAL
                     AGWWGETLCCLEVRGRSGGRVVTLASQVEQFPGQIDVFLADPDNRWPEFDRRQTVQAM
                     LQFAGTRYGYRNFLRVALRHIPGIRLIMSPKTDDSAMDTLGPICSQACVMATRLGGGV
                     DPVPQLADELTEPADLARSPFYRYRLTLIPDDVTQPTPEPQPSSAEPPRWLRLLTLVG
                     VFLLIFVLTGRATQGQSSCPGGYCYGPGAAGKASDTTPPAGGLSVSQPIPEPLSRATA
                     RLENHLPGGAIVYGSGVLIARDDRWGWVLTCDHLFREGVGQVRVVLPSSSGQVFEGRV
                     FARDSLADLALVGIAAPQTQPIALADTHPSPGQTVAWAGYGRTGRLHAAMGQVLGYVS
                     TNVSGNRETLQITGAAQDGDSGGPIVDAQGRLVAIVWGTDGYRTVGTYCGRIRAFLRN
                     FFAPREEQVPVLGPAVPPVPSNPPQDPPGNPHDPLPPGSSSGDSLLSRLRDRLEGLQG
                     KVEAAESGQRSLSERLRHLETTLGVVSDLRQRVEKAEEVVGRENIRAVIRETAADLLR
                     DRGPSWASALLSAVLTALGWTGPPSLALAFAARLAVRLLERRLRTSHRAEENVPSNPN
                     IVTQ"
     gene            1953..2210
                     /locus_tag="PLD05_03680"
     CDS             1953..2210
                     /locus_tag="PLD05_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76561.1"
                     /translation="MTPIDWGVLGAFLSTSILILLPWMLKVHARLAVALTRLENLESR
                     LAAMAEKMDELVQAEHHRATSEALWETRLQTLETRFGESEL"
     gene            2269..2835
                     /locus_tag="PLD05_03685"
     CDS             2269..2835
                     /locus_tag="PLD05_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76562.1"
                     /translation="MHIRDRIKELRRIPARQLRPNPRNWRVHPAKQQHALRGILAEIG
                     YADALLARELPDGTLELIDGHLRAETTPDAIVPVLVLDVSEAEANKLLALLDPLAGLA
                     EQDHDALAQLARDIEFESPVLQQLLAETTQAADNPPQESPPETETSAVDPVPELFQVL
                     VECRDEDDQRRLFERLSAEGYTCRLLTL"
     gene            2880..4052
                     /locus_tag="PLD05_03690"
     CDS             2880..4052
                     /locus_tag="PLD05_03690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP76563.1"
                     /translation="MKKHVVEVVLETPLDRSFRVMQVAGMFDLPLEKVTRERIAVEIP
                     QQTEDWQLGLIVGPSGSGKSTLARLLFGDRVSRTFTWPDDQAIVDAFGQWPIKEIVGL
                     LTAVGFSSPPAWLRPYRVLSNGEQARCDLARAFAEALQSGPVEDAQPWHVLLPPTADR
                     SRPLVVCDEFTSVVDRHVARAIAAGLAKRLRKGTLPCRFVAVTCHYDVADWLTPDWVI
                     DMATREFSRRSFRRPEITLAVVPCRREAWRAYRRHHYLSGSLPSAAESYLALWDGEAV
                     AFCAVASQIGRRHYRRISRLVVLPEYQGMGIGSAFLEAMARYYRKRGQRVGITTSHPA
                     MIAHLARSPQWRLRSLRKWGSRGGGRNLPQYRGSFGRAVMSFEFIGHASYRQPTAA"
     gene            4122..4616
                     /locus_tag="PLD05_03695"
     CDS             4122..4616
                     /locus_tag="PLD05_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76564.1"
                     /translation="MTETQGQETARRGLDEFRKREILAILSVGCSRRTAARYVGCSPS
                     TIRRTAERDPAFAEALHKAESKAQILFMQNIAAAARKEQYWRAAAWALERLNPEDYAA
                     RSPHAVTLEQIRTLVSEFARIIVEEVPIAAHRKKILRRLERLGREWDVPPSVAVNRTD
                     PSDE"
     gene            4630..6252
                     /locus_tag="PLD05_03700"
     CDS             4630..6252
                     /locus_tag="PLD05_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76565.1"
                     /translation="MPRNTFAESDLVLNQPQAAGMLTLLQSSLASLHGRTRRLHHSPC
                     EDQRFLDWAREYLPQHFRLPPSRMHRVVARHLEKAVAHRGMKLNVLGPRGSAKSTLVS
                     LAYPLWCLLEGKESYIWLVSDTRHQARAHLENIKAELLDNPQLCARYGPIRRKGTVWR
                     AEKVVLPSGAAIEAFGTGQRLRGRRQRADRPSLIIADDIQNDGHMTSSRLREHSRTWF
                     HGTLMAAGSPITNVVHLATALHYDALAMELSRTAGWQTLIFRAIERFPRRMDLWDRWQ
                     EIYCDADRPQAVHEARHFFLQHRREMEADAVVLWPEREDLYTLMCLRAEIGWAAFARE
                     KQNAPVSPELCEWPDEYFNEEIWYETTPRNLKLRVAVLDPSKGTDAKRGDYSALVFLG
                     VDAQGQMYVDADLARRPIAQMVTDAAEWCLRWRVQAFGVEANQFQELLAEPLEAEFRR
                     RGLMGFSPWLIRNHVNKLVRIRRLGPLLARRTLRFRQGSPGARLLVEQLRTFPIGDHD
                     DGPDALEMAIRLVGELARPAPDLSSTQRMVVW"
     gene            6324..7688
                     /locus_tag="PLD05_03705"
     CDS             6324..7688
                     /locus_tag="PLD05_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOP76566.1"
                     /translation="MLPYVRHEGKAPACVVRLAEIFHDPEDWDLEGRRLWWPLGCRST
                     SVPFQTETELDHIRSECRQLAVENEFAINGHENRISYVVGTGHRYQVMPRPGCRVPKS
                     ILAQAEAVLDRFLRENRWHARQQEIMRRKDRDGEAFLRFFPGDDGILRVRFINPEDIR
                     TPTQYSQRPEHSFGIATDPSDVETVHGYWVCEQWVPADQMQHRKANVDANVKRGLPLF
                     YPVRKNLRRAEKLLRNMSTVAGLQSAVALIRKHTGGRSSVERFLHDAAPPVPGEPSAS
                     TGYTSVQRFAPGTILDATAGVEYEFPVAAIDAARYVTLLQAELRAVASRLVMPEFMLT
                     SDASNANYASTLVAEGPAVKMFQRLQAEMIEEDHVVLERALQTAAHAGLLPVDVLQQV
                     TIKAVPPSLVVRDRLREAQADAILFRCGALSRETLALRHSLEPNTTNDSPMDESIRGE
                     QRNP"
     gene            7741..8616
                     /locus_tag="PLD05_03710"
     CDS             7741..8616
                     /locus_tag="PLD05_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76567.1"
                     /translation="MSHTHICEYLGWGDCPMKVDAHNGVIYGVKVLGLISRNGRRYSE
                     QALRQALPLYEGARVNINHPRGDAAAPRDYRDRLGFLRTVRYRDGEGLFADLHFNPKH
                     DLAERLAWDAEHAPHNVGLSHNVLARVEHQGQIPVVTEIHRVLSVDLVADPATTSGLF
                     EAKSPASSESAQAAAHGDGGEPPEMLELARLRGELALRRHIREWHLLPPDETASEGEQ
                     KMGPRLWEILISETDDSRRRQLLAELANLARPQTSHQTWQSSRPTSRDPLGVCECPNT
                     AAFVAAITGRGASWK"
     gene            8803..9282
                     /locus_tag="PLD05_03715"
     CDS             8803..9282
                     /locus_tag="PLD05_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOP76568.1"
                     /translation="MAHVMRWRYGDTQPVVATVDADSNIELGDLLFWDGDDAKPASLQ
                     PDQGTEAANQQLFASKFLGVAMQASPAGESSPIRVATAGVFEFTTPGGSFDLGDWVGV
                     DENDAGTVLENQRLVKVTSSQLAIGRVARRSMDGHTVWVAICSTVMAGGVKGTTATT"
     gene            9501..10673
                     /locus_tag="PLD05_03720"
     CDS             9501..10673
                     /locus_tag="PLD05_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76569.1"
                     /translation="MPAISYRELRRRYELDGAEKTIHHLSEALEKKHLRPDDFSLRDL
                     AESLIPEGRQWVKALDPRQSSPVSIMEAGDAVDVTAFLNVTGQIIYAQILETYRHEAF
                     VASRLVRTIPTRLDGEKIPGTSGIPETMTEVLPGMPYPHVGFSERYVETPSTTKHGLI
                     VAVTREAIFFDRTHIVLRQAAEVGETLGRLKEKRILDVIIGAVNTYRENGVAYNTYYA
                     AGSGGPWVNVLANNPLEDWTSVDRAEQLFSEMTDPLTGEPILIRPNTVLVPSALRHTA
                     RRVFHATAISFAPDGSATATTFANPLSDYRVWDSQLLYQRIVASGVSAEVAKTWWLIG
                     DFARAFAYMENWPLTVTQSAPGSEADFTQDIVVRFKASERGTAAVLDPRYVVRCSG"
     gene            10727..11539
                     /locus_tag="PLD05_03725"
     CDS             10727..11539
                     /locus_tag="PLD05_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOP76570.1"
                     /translation="MARLFNGTSQFLRRVDGTGLTLPLTLAAWVYPQRNTAYEEVLAL
                     ARSSDNSTGWFLQIRGPDGMRVAAVTASQNTFAIARSTGSYALNQWQHVAGVFSESNA
                     RRVYLNGVPGALDTTVLNVASVDRMLIGAWERLGGWLAYFGGAVAEAAIWQGALGDSD
                     LAQLAAGYSPLLVRSQDLIAYWPLGGPFGNNDQDHSGNGFDLTAYGDPTWADHPPIVY
                     PQPPEPPPAEAFLGVTGSVITAGAVAGMGFHAGNQAGSVHARLALGQVRIPR"
     gene            11570..11962
                     /locus_tag="PLD05_03730"
     CDS             11570..11962
                     /locus_tag="PLD05_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76571.1"
                     /translation="MSAAEIYGVVFQNATAAFLARVLDYDGQPVTPSQIASVEYTVAE
                     HLDDMTETTPIDGHTAVALNPSDVLFATLQKGGLWDVDDVGYNFRHVIDVSQAPAFPT
                     AGSHYRITYRLTPQTGQVIVVRFRVKAI"
     gene            12014..12229
                     /locus_tag="PLD05_03735"
     CDS             12014..12229
                     /locus_tag="PLD05_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76572.1"
                     /translation="MADDRDVIQLIRSQTLELIQQITAQPKPSYELDGQRVSWTEYLG
                     RLIEVVNWCDRQLAAETPCEIRSLGLT"
     gene            12241..12882
                     /locus_tag="PLD05_03740"
     CDS             12241..12882
                     /locus_tag="PLD05_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76573.1"
                     /translation="MRTDLIADDLSQFADRLETITHIRRGQNRGVTIQGAFRRAVPPR
                     REDRDDGWSGADMVWHLPEQAVPGGVRPGDHLVDGAQRRWTVLVVSPTASGRIIAWTR
                     DWSAAFSTGRTVDVERAEITQGASGEAIPRWTTAAAGVPAEFVSCHCDRSETGTDRLP
                     VYRVLVLWDGPQGRYRLKTDDGQYYSVLKVERPALLGHPTVFYVSPAPANHPT"
     gene            12926..13330
                     /locus_tag="PLD05_03745"
     CDS             12926..13330
                     /locus_tag="PLD05_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76574.1"
                     /translation="MITLEQALRTRWQDDPTLAALLPVNYVWTNWSDVAQVPRAEITA
                     AGEDRVWLTSRAEVAERVRVTVEVWHNSFEKLRSTINRIKELFDRASFDLGDNARILR
                     LTYQRDDMARQGYGLWRGEITFDGLALRPIVA"
     gene            13387..13896
                     /locus_tag="PLD05_03750"
     CDS             13387..13896
                     /locus_tag="PLD05_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76575.1"
                     /translation="MTTVLRSTLLAKLGWTWQDEQDRAVVADSNQWTWRTALNEGTQP
                     GEANAIWYAVGKTLASGASTEWLLDALSRPLFGKTIVQGFSSVKVLWLANRGESTGTL
                     VLGGASSNPWPGPLSSGSSTLCVAPGGVLFAVHPAAGWGVSSSAKAIKLLAEAGSVTY
                     DIILLGVAS"
     gene            13999..14847
                     /locus_tag="PLD05_03755"
     CDS             13999..14847
                     /locus_tag="PLD05_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76576.1"
                     /translation="MPVTFRELAGSPEETYTLEGFRARRTFLVPWEERHAFAAEILGS
                     GGDHGGRPWSRYPGKESVFAVAIRFSPADPEALRTLTEESPDVQEVLAEYSGSFAQAI
                     VEYTTITPEEREDTPSPPLQTHLSYRMEYGLMERPLLASGFFAEDDPQTPLAAELPLT
                     HIIPYTDHYLVWQQVINPPWQAIRELQGTVNEEVFLNATPGTLLFLGATTNKLFRGSF
                     DEGVSPFCWEIRYHFRELAIKHGGTVYGWNHLHRDQPAGYVAIADPEGPLYDSGDFSR
                     LFLPEV"
     gene            complement(15186..16388)
                     /locus_tag="PLD05_03760"
     CDS             complement(15186..16388)
                     /locus_tag="PLD05_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76577.1"
                     /translation="MSASPQAEFRKPLFWILAGVIVSVLVIALAVYLWPAPEVDVTYG
                     TMGWLGRKSINGTWVLAEMVRRSGHSVSRSTHLSPMLKEQADCLIWFAADPNVPHESV
                     VTWLEDWLKAKPGRTLIYVGRHFEATSLYWKKLLERFPDQYDPVRQKEIARRQALEEQ
                     FLQLISNVEGQCAWFSFKPLPDVVRPKSLEMHPQWRVPVNPQDLEIELRSQMIPAQDA
                     EVLIRSEKHVLVARLPRGSSQILLVNNGSFLLNMMLVNREHRKLARRLVQAIDESGTS
                     GPKRVCFLELRPGYQVVTGPTEEPSPYRFLTVYPLNVIVLHLFLLAVLFALARFPRLG
                     PGYEPDLVLRTSFDAHLAAMARWLRRTRDYQFVQEVISQWERISAPRGQGGRGLRERP
                     HKSRLLRY"
     gene            complement(16385..17080)
                     /locus_tag="PLD05_03765"
     CDS             complement(16385..17080)
                     /locus_tag="PLD05_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOP76578.1"
                     /translation="MGLLLAVGLLFAYGLVANATLAEAPEDLARQSLRQGNFPWYDAE
                     KDDIKPTRVRKPRELPEPSKVSRSRVPWSAVGIILIVLAIAAMVILGYWAWRRWAPCQ
                     GGKEEAARVAAQLRELPLVPEDSPQETDLLQASERAAAAGDYRRAIIWLYAYQLAELD
                     RHRLIRLMKGKTNRQYLRELGRESPVAPLLERTIVTFEEAYFGGRSLSAEQYEACRRE
                     LPQFQQWLKEIAA"
     gene            complement(17350..18258)
                     /locus_tag="PLD05_03770"
     CDS             complement(17350..18258)
                     /locus_tag="PLD05_03770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP76579.1"
                     /translation="MDGTVLGASENPSFEPFVTDDYELIDFGDSRRLERFGSVILDRP
                     SPAAEGPPLYPDRWSQAHARFERTRGNRGRWVDRCHVPERWTIRWGPLVLELKRNESG
                     HVGLFPEQVVNWSWLYHHITQSEKVGPVLNLFAYTGGSTLAAALAGAEVVHVDAARNV
                     VAWAKRNAVLSGLENLPIHWIVEDARKFLRREVRRERRYWGIILDPPTYGHGPRGESF
                     QLEKDIVGLLEAVFALLDWDRGFLLMTCHTPGYGTQKLTRLFHDLRPDLWQRGQITVR
                     MMTIPSSSGRPLPSGMVVRWQSSPPR"
     gene            complement(18281..18544)
                     /locus_tag="PLD05_03775"
     CDS             complement(18281..18544)
                     /locus_tag="PLD05_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76580.1"
                     /translation="MAEIPVGRVTHYFSHLGVAAIEITHGTLSVGDTIHIKGHTSDFY
                     QPVTSMQIEHQTVTQAQAGQSVGIKVVEHAREHDVVYKVVPEE"
     gene            19363..20595
                     /locus_tag="PLD05_03780"
     CDS             19363..20595
                     /locus_tag="PLD05_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76581.1"
                     /translation="MDCFRFLHTGELWLDRPIRVRGGLPPSLMARVLEGPRRTWERIC
                     QLALREDVDLVVLSGNLVDGSRASPADLDAVLAGCETLAQNDIAAIWMAGKAEEQGPW
                     PAELALPDTVHVFSGEEVQQRQVRTRKGTIDVLVWPTSYPWEASREAFLPHQPGQFVL
                     GLARCQSLEELPNIIGVSYWALGGPVPRTDSLPSTVAHAAGPPLIRDADDPALPTVTI
                     AEWNINRRIALREVSVAAIRYATVVLLISPETQWAGLSSQLVQELESRRASSDADWLI
                     AIKLQGPVEALIRWKREGLDAQLLEFVQSRYLQEPPYLWPTDVVWDPVDLGPDEWLKE
                     TSFRGEFLRQVHAIYENLVIGQTDAVAALAGPISPGQAVDAIVPIWEEILRQAAFEGL
                     EALSHVSASVKGEASACS"
     gene            20586..24098
                     /locus_tag="PLD05_03785"
     CDS             20586..24098
                     /locus_tag="PLD05_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP76582.1"
                     /translation="MLLKRLAIDRFGIWHNWEVEDIPRGLAVFFGPNETGKSTILEFV
                     RGMFFGLAARSRFLLGEQGDIGGTLVLEHLGREVRLSRHWSQDEGERVQVVVDGQPVL
                     PEELMRVLCPVDEATFNAVFALGLEDLAYLRTLEEGETAKLLYELSLGADRAVLWRVL
                     TNLHQRLAQDQDDTELKELEAERARLVRTTEEAGLETRQYVRWRVTQRKLQAERQTVM
                     QRLDSQQREQQRCEAVLDLLPKWQQYREMRLQWKRLGPSRRISEKALRKLDRWKERMT
                     AAKHNLAEAREALGQYDEALAGLPVDPVVLEHAAEIEALRLHRDEMVACKERLEELNR
                     RQQELLQARSALWEGLASVTQIGNISTPNDGLPQADQRGTAQDWSALRGWIRRWQKAV
                     LERDRLAEEDQRQRAAQNALKEQVEAALQKHGVADAQVALSQYSEQVTGLRRVRQLAE
                     QQAAVKQRVEQVTTEYHRQVAQSLPSWQTWAGVGSLFIPGLTLVFLSLLALLGGPGAG
                     NLGLVTLLLGAAMTGGGIGLKFYHEHRQRVNLENLKKDLETLTTELQSVSQEYQRLAA
                     EQNVSQADLDQLISQLEGRIRELEPVAVLQAKFAEMTQPGQTHRETLHRLEQKERAIR
                     AKFNELAHRLGLPSADDVTQALQIWEVGHRLRALDRRLTVVTAKLKKEEERLAAWQKR
                     VLRLARLCGRSDETDDVVQVLDGLVRHYEIHLENQRRRQDLLRTAKQTKQKVHRAKRR
                     LKKIALRYRATLRQLGVRNPQEATALRQTWEQAQTLRRQAGEIHRELKAALAAAGIAE
                     DVPLARLFQETKAKHAELIQAMEATKEELHALEEKQAHVEAELARLVQSRDVDQAKLA
                     LAALEHQIVRRVRRQQTSRLLAELLERVRQHYELENQPETLRRASVFLAQMTEGRYVR
                     VWTPISERVLLVEDATGKNWRVDELSRGTREQLFLSFRLAIVERSAEQGIRLPLVLDD
                     VLVNFDFQRAAAACRTLRAFAEASHQVLLLTCHDHIARLCTEMSVPVAHLETTGGGKA
                     TPPFFRGYFPPLKTADTPIAPTTPETPRLSGSQEEKTVADGREKVLSQEPSRSRRRRK
                     QTPNGKAVETEKVLGDHKQPGEMASPSAGQVFFQSNCTWLTEAEPYADVNEEGNPQGE
                     AKGDRSSAEPVGNHRAKQRRARAA"
     gene            complement(24138..25214)
                     /gene="purM"
                     /locus_tag="PLD05_03790"
     CDS             complement(24138..25214)
                     /gene="purM"
                     /locus_tag="PLD05_03790"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOP76583.1"
                     /translation="MGKVTYKSAGVDLDLYRQAMARIPQLLERTVTPRVINLPFGFAG
                     LFRLDFPWPLFARNYKDPVLVACTDGVGTKLKVAAMMGIFNTVGIDLVAMSVNDALCC
                     GAEPLFFLDYVAMPKDDPPLLEKLLEGIVNGCREADCALLGGETAILPDMYQPGDFDL
                     AGFCVGVAEARAIITGQAITPGDAVLGLASSGLHSNGFSLVRKTVFDLAGLRVTDHVP
                     ELGTTVGEALLVPTRIYVRPIRSVLRYYRVKSVVHGIAHITGGGIRENLARIVPPGVR
                     IVIDRDAWPVPPIFRWIQKLGETDDAEMENVFNLGLGMILVVSDYYAASIRKQLEDLG
                     VPCWQIGRAEAADESGVVWAQSSA"
     gene            25894..26733
                     /locus_tag="PLD05_03795"
     CDS             25894..26733
                     /locus_tag="PLD05_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOP76584.1"
                     /translation="MAVFARLWPVGLLLVGTIVGNGCATNPATAAAQNQAAQQQAALA
                     RQIEEYQTRSTALDRDNQQLQQLLAQAYQKNKLLEDQVGLLQKQLADMNNQIARLQQE
                     KAEQQKQVQTLTASLQRSGSVTIQPNNSLLQTLPAINIAGVQVRRDGDVIRVELPADQ
                     LFETSTFQFRPGAVQMITSVAAEIARLYPEQIIGVEGFTDTDPVPPGLWRNHTQMSIS
                     WAAAVHDVLVTQTALRPEQLFVAGHGSAYPIMSNGTAAGRARNRRVELVIYPEKYRSP
                     GRS"
     gene            complement(26739..28028)
                     /gene="aroA"
                     /locus_tag="PLD05_03800"
     CDS             complement(26739..28028)
                     /gene="aroA"
                     /locus_tag="PLD05_03800"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOP76585.1"
                     /translation="MMAEASDFVEIPLSGPVNATVQPPGSKSITNRALICAALADGPS
                     LLQGALDSEDTQVMVRALQSLGVAVRHDTKQREIAVEGTRGVIPARQADLFLANSGTS
                     LRFLTAICTLGEGQFRLDGTPRMRERPIQDLVDALNPLGANVRTELPTGCPPVIVEAH
                     GLPGGETEISGEISSQFLSGLLMAAPYARRDVTLLVRGDLVSKPYVEMTLAVMKAFGV
                     TGENEAFQRLSVRSGQMYRGRTYAIEPDASAATYFFAAAAVTQGRITVTGLGENSLQG
                     DVRFVDCLAAMGCEVTMGDDQITVEGRPLRGISVDMNAISDTVPTLAVVALFAEGPTL
                     IRGVKHIRHKETDRIHALALELRRLGAQVEEFEDGLRIIPASYHPAEIHTYQDHRMAM
                     SFAIAGLRIPGIKILDPGCTAKTYPEFFEDLRAIIRP"
     gene            complement(28131..29396)
                     /locus_tag="PLD05_03805"
     CDS             complement(28131..29396)
                     /locus_tag="PLD05_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76586.1"
                     /translation="MIKVRMHIYLAALLVGACCGTWELAQGEDWPAFRGGLAAGVLCG
                     GEDIPPVLSPENQLLWKAPLPGRGPSSPIVVKDKVIVTAAVGYRQNELHVLCFDAKTG
                     KQEWDFRFWATGSTVCNPFGGVAASTPVSDGRRIVALFSSNDLVCLDMEGRPLWFRGL
                     GFERPYLRNDVGMASSPVIEGDVVIVQAESLLDAIVCGLDLKTGQTVWLIDRPKQALW
                     CTPLVVRCPSPSERTPETFVLLQSRKDFQAVCPYTGEVAFAYDHWCDTVSSAAAYGDL
                     VALPAAGIHALRLSSSPARFEQLWISDRLRCGNTSPLVWNNKIYLVKSPNIFACADGK
                     TGDILRQVRLGGSFWASPLLVGRYIYAVNYEGTVFVMALDDAGLPSLAGQWDLESGVL
                     ASPASDGRQLYFRTNQFLYAFRLGDSGKE"
     gene            complement(29434..30606)
                     /locus_tag="PLD05_03810"
     CDS             complement(29434..30606)
                     /locus_tag="PLD05_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP76587.1"
                     /translation="MTRIKLSRPVPWQDVVFALGLVLGLLAAQYATGDIPGDIAILLS
                     SGQSTTKPEDVSEAWKRLVSAGPDALGPLFVAARQADPASRNWLLSAADQIVAREHGA
                     GRQLSQEVILKLLEDTQNPPQGRWWAYQWLARQDPAQARQILIRFLNDPASELRRAAI
                     EAELQALGDIQNVPVNDPKNVEIVRKLKQLFDAARDQDQVENLAEMLRKRGVSVDLAG
                     HYGYLRTWWIVGPFDNQQDVGFDAIYPPEGQSPPDFSATYQGKHGPVTWKKVTTPDEH
                     GTLDLNKVLGEEKGVVAYAVAALVSQTEQAAEIRAATPNAIKIWCNGQPLGAFHVYHS
                     GFEDDQYRVVCRLQPGVNWIMIKVCQNEQTQDWAQYWTFRLRVCDELGKGLGRQPD"
     gene            complement(30641..31912)
                     /locus_tag="PLD05_03815"
     CDS             complement(30641..31912)
                     /locus_tag="PLD05_03815"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOP76588.1"
                     /translation="MQNVLTIVLGGGRGTRLYPLTKFRSKPAVPIAGKYRLIDIPLSN
                     CINSDLSRIYVLTQFNSASLHRHIRATYNFDVFHRGFVEILAAQQTLTSSDWYQGTAD
                     AVRQNLAYINRDDVDYVLILSGDQLYRMDFRELIAAHEKKGADVTIAALPVMKRAASS
                     LGIMWIDEHGQVLGFLEKPQTEEELRYMQTDPDWLSQFGIQAEGREYLANMGIYLFSR
                     KTLVDVLEMTDYRDFGKEVFPASIRTRRVFVYLFDGYWEDIGTIGAFFEANLALTKPN
                     AAFSLEHPNAPIYTRARFLPPSRIESATIQSSLVADGCIIGSGSVIENSIIGLRSVIG
                     RNCVIRNSIIMGNDFYDLEQTRDGKPPMIIGEGSYIENAIVDKNCRIGKNARIVNTTG
                     LTEMPENQYCMIRDGVVVIPKETILPDKWCL"
BASE COUNT         6584 a         9703 c         9308 g         6406 t
ORIGIN      
        1 gggcgacagg cccggtgact ctgttacccc cgatgttcaa cggagggccg cctgatgacc
       61 caatttcgtt acctcgtgcc gctacaccaa gcccaggact ggattcgcga cggcgattta
      121 ctcctctttc ggcgccgcgg tctgattgcc gctgcgggtc ggggggagca ttcccacgct
      181 gctttggccg gttggtgggg ggagaccctc tgctgcctcg aagtgcgggg acgttccggc
      241 ggtcgtgtcg tcacgttggc cagccaagtc gaacaattcc cagggcaaat cgatgtcttt
      301 ctcgccgatc cggacaaccg ctggccagaa ttcgaccgcc gccagacggt gcaggccatg
      361 ctgcaattcg cgggaacacg ctacggctac cggaattttc tccgcgtggc cctccgccac
      421 atcccgggga taagactcat catgtcaccc aaaacggatg attccgccat ggacacgctg
      481 ggtcccattt gcagccaagc ctgtgtgatg gccacgcggc tcggcggtgg tgtggacccc
      541 gttcctcaat tggccgacga attgaccgaa ccggccgacc tcgcccgcag ccccttctat
      601 cgttatcggc tcacgctcat ccctgatgat gtgacccaac cgaccccgga gccgcagccc
      661 tcctccgccg aacccccgcg atggctacga ctgctgacac tcgtcggtgt ctttcttctc
      721 atctttgttt tgacgggaag agcgacccag ggccaatcct cgtgtcccgg cggttactgt
      781 tatggccccg gtgcagcagg caaggccagc gataccacgc cccctgcggg tgggctttcg
      841 gtttcccagc cgatccccga accgctgtca agagccaccg cccggctgga gaatcatctc
      901 cccggtgggg ccatcgtgta cggctccggc gtcctcattg cccgggatga tcgctggggt
      961 tgggttctaa cctgcgatca tctcttccgc gaaggcgtcg gccaggtcag ggtggttctg
     1021 cctagctcca gcggacaggt attcgaaggc cgcgtctttg cccgcgactc gctggccgac
     1081 ttggccctcg tcggtatcgc cgccccgcag acccagccca ttgccctggc cgacacgcat
     1141 ccctccccag gccaaaccgt ggcctgggcg ggttacggcc ggacgggtcg cctccatgcc
     1201 gccatgggcc aggtcctcgg ctatgtcagc acaaatgtct cagggaatcg ggaaaccctc
     1261 cagatcacag gtgcagcgca agatggtgat tcgggtgggc ccatcgtgga tgctcaagga
     1321 cgtttggtgg ccatcgtctg gggcacagat ggttatcgca ccgtgggcac ttactgcggg
     1381 cggattcgag cctttcttcg caactttttc gctccccgcg aggagcaggt gccggttctc
     1441 ggtcccgccg taccgcctgt cccatcaaat cctccgcagg acccgccagg aaatcctcac
     1501 gatcccctac cgccagggtc gtcctcagga gattcccttt tgagtcgatt acgcgatcgg
     1561 ctagagggcc ttcaaggaaa ggtcgaggcc gccgaatccg gccagcggag cctcagcgag
     1621 cgccttcgcc atcttgaaac gacgcttggc gtggtcagcg acctgcgtca gcgggtggaa
     1681 aaggcagaag aagtggtcgg gcgggaaaac attcgggccg tcatccgcga aacagcggct
     1741 gacctgctga gggatcgggg gccctcctgg gcaagcgccc tcttgtcggc cgtgctcact
     1801 gccctgggtt ggaccggccc tccttcgctt gccctggcct ttgccgcacg actggcggtg
     1861 aggctcttgg aacgccgtct ccgcaccagc caccgcgcag aagaaaacgt tccatcaaac
     1921 cctaacatcg tcactcagta aaggagaaga caatgacgcc cattgactgg ggtgtgctcg
     1981 gcgcattcct tagcaccagt atcctcatcc tccttccctg gatgttgaag gtccatgccc
     2041 gactggccgt ggccttaacc aggttggaaa acctggaaag tcgcctcgcc gccatggctg
     2101 agaagatgga tgaactcgtc caggcagaac accatcgggc gacaagcgag gccctttggg
     2161 aaacccggct gcaaaccctc gaaacccgtt tcggtgaaag tgaactgtga cggaaaagaa
     2221 cagaccactt cgccaggata agccacagaa acagctaagg taggtcccat gcacatccgt
     2281 gatcgaatca aggaattgcg gcgtattccc gcccgccaat tgcgccccaa tccccgaaac
     2341 tggcgtgttc atccggccaa gcagcagcat gctttgcgcg gcatcctcgc cgaaatcggt
     2401 tacgccgatg ccctgttagc ccgcgaactc cccgatggta cccttgaatt gatcgacggc
     2461 cacctccgcg ccgagacgac gccggacgcc attgttcccg tgcttgtcct cgatgtctcc
     2521 gaggccgagg ccaacaagct actcgctctt ctcgatcccc tggccggtct ggccgaacaa
     2581 gaccacgacg cccttgccca acttgcccgt gacatcgaat ttgaaagccc tgttctccaa
     2641 cagctccttg ccgaaacaac gcaggctgcc gacaacccac cacaggagtc gccaccggag
     2701 acggaaacga gtgccgtcga tcccgtcccg gagttattcc aagtcctagt ggaatgccgc
     2761 gatgaggacg accagcggag gctgtttgaa cggctttccg ctgagggcta cacctgtcgc
     2821 ctcctgacgc tctaagccac tgccgatttg ctacgtccgc cttaaattgc gagtcaccca
     2881 tgaaaaagca cgtcgtcgaa gtggttctcg aaacgccgct cgatcggtcc ttccgcgtca
     2941 tgcaggtcgc cgggatgttt gatcttcccc tggaaaaagt gacccgcgag cggattgccg
     3001 tcgagattcc ccaacaaacc gaggactggc aactgggctt gatcgtcggg ccgtcgggaa
     3061 gtggcaaatc gaccctcgcc cgcctgctat ttggcgatcg cgtcagccgt actttcacct
     3121 ggcccgatga ccaagccatc gtcgatgcct ttggccaatg gcctatcaag gagatcgtgg
     3181 ggcttttgac ggccgtgggc ttcagttcgc ccccagcgtg gttgcggccg taccgggtcc
     3241 tcagcaacgg agaacaggcc cgctgtgatt tggcccgcgc ttttgccgag gcccttcaat
     3301 cgggtccggt cgaagatgca cagccttggc acgtccttct cccacccaca gccgatcgca
     3361 gccggccgct tgtcgtctgt gacgaattca cgagcgtcgt tgatcgccac gtggcccgag
     3421 ccattgccgc gggcttagcc aaacggcttc gaaaggggac acttccctgc cgatttgtgg
     3481 ccgttacctg tcactacgat gtggccgact ggctcacccc ggattgggtc atcgatatgg
     3541 ccacgcggga gttttctcgg cgatcctttc ggcgcccgga aatcaccttg gcagtcgttc
     3601 cttgccggcg tgaagcgtgg cgggcctatc gccgtcatca ctatctaagc ggcagtctcc
     3661 cctcggccgc cgaatcctat ctggccctat gggatggcga agccgtggcc ttctgtgccg
     3721 tggcttccca gatcggtcgg cggcactacc gccgcatcag tcgtttggtt gtcctcccgg
     3781 aatatcaagg gatggggatc ggctcggcat ttctcgaagc gatggcacgt tactatcgaa
     3841 agcgggggca acgtgtggga atcacgactt cacatcccgc catgattgcc catcttgctc
     3901 gttcgcccca atggcgactg cgttccctcc gcaaatgggg ctcgcgcggt ggtggacgaa
     3961 acctgccgca ataccgtggt tccttcggtc gtgcggtcat gtcctttgag ttcatcggcc
     4021 atgcctcgta ccgacagccc accgccgcgt aatggcgaag ccgttccaga ggatcaggaa
     4081 ccgcggaaac gacctaagac tcttggcgga aggaaacagt tgtgacggaa acccagggac
     4141 aagagacagc gcgtcgcggt ttggacgaat tccgcaaacg ggaaattttg gcaatcctca
     4201 gtgtgggttg tagtcgtcgc acagcggccc gttatgtggg atgttcaccc tccacgattc
     4261 gccgcaccgc cgaacgtgat cccgcctttg ccgaggccct ccacaaggcc gaaagcaagg
     4321 cccaaatcct ctttatgcaa aacatcgcgg cagctgcccg caaagaacag tactggcgtg
     4381 ccgcggcctg ggcgctggaa cgcctcaacc cggaagacta cgccgctcgt tccccccatg
     4441 ccgtgacatt ggagcaaatt cgaaccctgg tctcagagtt tgcccgcatc atcgtggaag
     4501 aagtccccat cgccgcccac cggaaaaaga ttctccgccg cttggaacgc ctgggtcggg
     4561 aatgggatgt ccctccctct gtggccgtca atcgcactga cccttcggac gaatgaaagg
     4621 acgcccaaga tgccgcggaa cacatttgcc gagagtgacc tggttcttaa ccagccgcag
     4681 gccgcgggga tgctcacctt gttacaatcc tccctggcaa gcttgcacgg ccggacccgg
     4741 cggctgcacc attcgccctg tgaagatcaa cgattcctcg attgggcccg agaatacctg
     4801 ccgcaacact tccggttgcc accttcgcgc atgcatcggg tcgttgcccg ccatttggaa
     4861 aaggccgtcg cgcaccgcgg gatgaagctc aatgtcctag ggccgcgggg gtctgccaaa
     4921 tcgacattgg tttccttggc ctatccccta tggtgtttgt tggaaggcaa agaaagctac
     4981 atctggttgg tctccgatac tcggcatcaa gcgcgtgccc atttggaaaa catcaaggcc
     5041 gaactgctgg acaaccctca gctttgcgca cgatacggac cgatcaggcg gaaaggcacg
     5101 gtctggcggg cagaaaaggt tgtccttccg tcaggggccg ccattgaggc cttcggcacc
     5161 ggccaacgct tgcggggacg acgacaacgc gccgatcgcc ccagcctgat catcgccgat
     5221 gacattcaaa acgacggaca catgacgtct agccgcttgc gggaacattc acggacctgg
     5281 ttccacggca cgttgatggc cgcgggcagc ccgatcacca acgtcgttca cctcgctacg
     5341 gccttgcact acgacgccct ggccatggag ttatcccgaa ccgccggttg gcagacgctg
     5401 atctttcgcg ccattgaacg ctttccgcgg cgaatggacc tttgggatcg ctggcaagaa
     5461 atctactgcg acgccgatcg gccccaggcg gtccatgagg cccgtcattt cttcctccag
     5521 caccgtcggg aaatggaagc cgatgcggtc gtcctttggc cggagcgaga agacctttac
     5581 accctgatgt gcttgcgggc tgagatcgga tgggcggcct tcgccagaga aaaacaaaac
     5641 gctccggtct cgccagaatt gtgcgaatgg cccgacgaat acttcaacga agaaatttgg
     5701 tacgaaacga ctccgcgcaa tctaaaactg cgagttgccg tccttgatcc cagtaagggg
     5761 accgacgcca agcgtggtga ttactcggcg ttggtctttc tgggagttga tgctcagggc
     5821 cagatgtacg tggatgcgga cctggcccgt cgcccaattg cccaaatggt caccgatgcc
     5881 gccgagtggt gtctccgctg gcgggtccag gcctttggcg tggaggccaa ccagtttcaa
     5941 gaactccttg ccgagccgct ggaagcggag tttcgtcgcc gtgggctcat ggggttttca
     6001 ccctggttaa tccgcaacca tgtcaacaaa ctggtgcgga ttcgtcgttt ggggccgctt
     6061 ctcgcgcggc gaacacttcg cttccggcaa ggctcgcccg gtgctcgact cctggtcgaa
     6121 cagctccgca cctttcccat tggtgaccac gacgatggcc ccgacgcctt ggaaatggca
     6181 attcgcctcg ttggcgaact cgcgcgaccg gcccccgacc tctcctccac gcagcggatg
     6241 gttgtctggt gatcgtagcg attgccttgg ttgtttcctg ttcgagccat cacgacggtc
     6301 agtaaaagaa gaaaggaaga accatgctgc cgtatgtccg gcacgagggt aaggcccccg
     6361 cctgtgtcgt tcgtttggcc gaaatcttcc acgatcccga agactgggac ctggaaggcc
     6421 gtcgcctttg gtggccgctg ggctgccgaa gtacgagtgt tcctttccag acggagacgg
     6481 aactggacca catccgcagc gaatgccgtc aactggccgt ggaaaacgag tttgccatta
     6541 acggccacga aaaccgaatc agttacgtgg tgggcaccgg ccaccgctac caagtgatgc
     6601 cgcgaccggg gtgtcgtgtg ccgaagtcca tcctcgccca agcagaggcg gtcctcgatc
     6661 gattcctccg cgaaaaccgc tggcacgcac gacagcagga aattatgcgt cgcaaagacc
     6721 gcgacggtga ggcatttctc cgctttttcc ccggcgatga cggtatcctg cgcgttcgtt
     6781 tcatcaatcc agaggatatc cgcacgccga cccaatacag tcagcggcct gagcacagct
     6841 tcggtattgc tactgatcca agtgacgtag aaaccgttca cggttactgg gtttgcgaac
     6901 aatgggtccc cgccgaccag atgcaacatc gcaaggccaa cgtcgatgcc aacgtcaaac
     6961 gcggtctgcc gctgttttat ccggtgagga agaaccttcg ccgggcggag aaactgctga
     7021 gaaacatgag caccgtggcc gggctccagt cggccgtcgc cctgattcgc aaacatacgg
     7081 gcggacgcag cagcgtggaa cgctttctcc acgatgccgc cccgccggtt ccgggcgagc
     7141 cgtccgccag caccggttac acctcggtgc aacgttttgc cccgggaacc attctcgacg
     7201 ccaccgcggg agtggaatac gagtttcccg tggccgccat cgatgccgcc cgctatgtca
     7261 cacttctcca agccgaattg cgggcagtgg ccagccgcct cgtcatgccg gagttcatgc
     7321 tcaccagtga tgccagcaac gccaattacg cctctacatt ggtggcggaa ggtcccgccg
     7381 tcaagatgtt ccaacgactt caggccgaga tgatcgagga agatcacgtc gttctggagc
     7441 gggctttgca aacggcggcc cacgccgggt tgctcccagt cgatgtcctc cagcaggtca
     7501 ccatcaaggc tgtgccgcca tcgttagtgg tccgcgatcg cctcagggag gcccaggccg
     7561 atgccatcct cttccgctgt ggtgccctct cccgcgaaac cctcgccctc cgtcatagct
     7621 tggaacctaa caccacaaac gattccccga tggatgaaag catcagagga gaacaaagga
     7681 acccgtaaaa accgttgtca cagattttct ctcgttttcg tgtcttaaag aggtccaacg
     7741 atgagccaca ctcacatttg cgagtatctt ggatggggcg actgtcccat gaaggtggat
     7801 gcccacaacg gggtcattta cggggtcaag gtcttgggcc ttatctcccg caacggacgc
     7861 cgctacagcg agcaagccct tcgccaggcc ttaccgctgt acgaaggggc cagggtcaac
     7921 atcaatcatc cgcgtggcga cgccgcagcc ccccgcgatt accgcgatcg cttgggattt
     7981 ctccgtacgg tgcggtatcg agatggagaa ggcctctttg ccgatctcca cttcaacccc
     8041 aaacacgacc tggccgagcg acttgcttgg gatgccgagc acgcccccca caacgttggg
     8101 ctatcccaca atgtccttgc ccgagtagaa caccaaggtc aaatcccggt tgtcacggag
     8161 attcatcgcg tcttgagcgt cgatctcgtg gccgaccccg cgacgacctc aggccttttc
     8221 gaggccaagt cccccgcgtc gtccgaaagc gcccaagccg cggctcatgg ggacggcggt
     8281 gagcctcccg aaatgctgga gttggcccgc ttgcggggtg aattggcgct gcgccgccac
     8341 atccgagagt ggcatttgct cccgccggat gaaacggcct cggagggcga gcagaagatg
     8401 ggaccacggc tctgggaaat cctcatctcg gaaacagatg actcgcgacg gcggcagctc
     8461 ctggccgagt tggccaacct ggcgcgtccg cagacgtccc atcaaacgtg gcagtctagc
     8521 cgcccaacaa gtcgagaccc ccttggggtg tgcgaatgtc ccaataccgc cgcctttgtg
     8581 gcagcgatca ccggccgagg tgcctcatgg aagtaaatgg gagaaaagac cggccgagca
     8641 ttactcggcg ccaaacgtac cgatcttcaa ccaggtttga ctctttacac gactgcgaag
     8701 cccctctgcc cgtctctgcc ggagatggca cttgaggaag cagcgacgtg gcctgggcca
     8761 ctgttgacct tcaacacgtt taccgagtga aaggagattt ttatggctca tgtgatgcga
     8821 tggcgttacg gcgacacgca acccgtggtc gctacggtgg atgcggacag caatatcgaa
     8881 ttgggcgatt tgctgttttg ggacggcgac gacgccaaac cggcgtcgct ccagcccgac
     8941 caggggaccg aggcggccaa tcagcagttg ttcgcctcca agttcttggg cgtggcgatg
     9001 caggccagtc ccgccggtga gagtagtccc atccgggtgg ccaccgctgg ggtattcgag
     9061 ttcaccacgc ccggcggttc gtttgacctg ggtgattggg ttggcgtcga tgagaacgac
     9121 gccggaacgg tcctcgaaaa ccaacgcctg gtgaaagtca ccagttccca attggctatc
     9181 ggtcgagtcg cacgccgttc gatggacggt catacggtgt gggtcgccat ttgctcgacc
     9241 gtcatggctg gtggtgttaa aggcacaacc gcaaccacct aagagcttat cccacaacct
     9301 cccctctccc gttcggcggg agtagggcct ggggtgaggg tggctcgaat cgctgccggt
     9361 ttctcgatcg actccgacgg tcgcttgccc tcaccccaac cctctcccag ggggagaggg
     9421 aaatttggga taggcttcaa acgtgtagct tcttggtatt tgaggatcca cccatttggt
     9481 tctttgatca ggggtaaatc atgcctgcga tttcctatcg tgaactgcgt cggcgctacg
     9541 aactggacgg tgccgagaag acgatccatc atctcagcga ggccttggag aagaagcatc
     9601 tccggcccga tgacttcagt ctccgtgatc tggccgagtc cctcattccc gaaggccgcc
     9661 agtgggtcaa ggccctcgat ccccggcagt cctcgcctgt ctccatcatg gaggccggtg
     9721 acgccgtgga cgtgacggcg ttcctcaacg tgacgggaca gatcatctac gcccagattt
     9781 tggaaaccta ccgccatgag gcgtttgtgg cctcccgctt ggtgcggacc atccccacgc
     9841 ggctggacgg agaaaaaatc cccggcacgt ccggcatccc ggaaacgatg acggaagtcc
     9901 ttcccggcat gccgtatcct cacgtcggct tcagcgagcg ctacgtcgag acgccctcca
     9961 caaccaagca cggcctgatc gtggcggtca cccgggaagc gatcttcttt gatcggaccc
    10021 atatcgtcct ccgccaagcc gcggaagtgg gcgagacact cggtcgcctc aaagaaaagc
    10081 ggattctcga tgtcatcatc ggtgctgtga acacctaccg ggaaaacggt gttgcgtaca
    10141 acacctatta tgccgcggga tcgggcggcc cctgggtcaa cgttctcgcc aacaatccgc
    10201 tggaggactg gaccagtgtc gatcgggccg aacagttgtt ttcagagatg accgacccgt
    10261 tgaccggcga acccattctc atccggccaa acacggtgtt ggttcccagc gccctgcggc
    10321 acacagcccg acgggtcttc cacgccacgg cgatctcctt cgcaccagac ggttcggcca
    10381 cggccaccac gtttgccaat ccgctgtcgg attaccgcgt ctgggatagc cagttgctct
    10441 accaacggat cgtggcatcg ggagtcagtg cggaagtcgc caagacatgg tggctgatcg
    10501 gtgattttgc ccgggccttt gcctacatgg aaaattggcc gctgacggtg acgcaatcag
    10561 cgccgggaag cgaggccgat tttacccagg acatcgtcgt ccgtttcaag gccagcgagc
    10621 gggggaccgc ggcagttctc gatcctcgtt acgttgtccg ctgtagcgga taagaaagtg
    10681 aaagcgttga tgcccgatcc acgccaacac attacgaggc taccccatgg cacggttgtt
    10741 caatggaacc agtcagtttt tgcggcgggt cgatgggacg ggcctgacgt tgccgctcac
    10801 cctagccgct tgggtctatc ctcagcggaa cacggcctac gaggaggtcc ttgcccttgc
    10861 ccgatccagc gataacagca ccggctggtt tctgcaaata cggggacccg acgggatgcg
    10921 tgtggccgcc gtcacggcct cgcagaacac cttcgccatt gcccgcagca ccggcagtta
    10981 cgcccttaat cagtggcaac atgtggcggg ggtgttttcg gagtcaaatg cccgacgtgt
    11041 ctatctcaac ggcgttcccg gcgcgttgga cacgactgtg ctgaacgtgg cctccgtgga
    11101 tcgcatgctg atcggcgcat gggaacgact gggtggatgg ttagcctatt ttggcggtgc
    11161 cgtggccgag gccgccatct ggcaaggggc cctcggtgat tccgacctcg cccaattggc
    11221 cgcaggttat tcgcctctat tggttcgttc gcaagatttg attgcctact ggcctttggg
    11281 cggacccttt gggaataacg accaggatca ctcgggcaat ggcttcgact tgacggccta
    11341 cggtgatccc acctgggctg accatccccc aatcgtctat ccccagccac cggaaccgcc
    11401 accggcagag gccttcctcg gtgtcaccgg gtccgtgatc acggcggggg ctgtggctgg
    11461 tatgggcttt cacgcgggga accaagcggg atcggttcac gcccgccttg ccctcggcca
    11521 ggtccgcatc cctcgttgat atcttcttca ccagaaacag gagccaaacg tgtccgccgc
    11581 tgaaatttat ggcgtcgtct tccaaaatgc cacggcagcg ttcctcgccc gcgttttgga
    11641 ctacgatggc caacccgtca cgccatctca aattgcgtct gtcgagtaca ccgtggctga
    11701 gcaccttgat gacatgaccg agacgacacc gattgacggc cacaccgccg tggcgctcaa
    11761 cccaagtgat gtcctctttg ccaccttaca gaagggcggc ctttgggacg tggatgacgt
    11821 ggggtacaac ttccgccacg tgattgacgt ctcgcaggcc ccggcctttc ccacagcggg
    11881 gtcccattac cgaatcacat atcgcctgac accacagacg gggcaggtca ttgtcgtccg
    11941 ctttcgggtc aaagcgatct agatccgaca cacgtggtca agtcttccca gcatagcaaa
    12001 ggaggaactc cccatggctg atgatcgaga tgtgatccag ctcatccgca gtcaaacgct
    12061 ggagcttatc cagcagatta cggcccagcc caagccgagc tacgagcttg acgggcagcg
    12121 ggtctcttgg accgaatatt tggggcggct catcgaggtc gtcaactggt gtgaccgaca
    12181 acttgctgcc gagacgcctt gtgaaatacg ctccttggga cttacgtgag gacacacacc
    12241 atgcgcacag atttgattgc cgacgacctt tcccaatttg ccgatcgact tgaaaccatc
    12301 acccacattc gccgggggca aaaccgcggg gtcaccatcc agggagcctt ccggcgggcc
    12361 gttcccccac gccgcgagga ccgcgatgac ggttggtcag gggccgacat ggtttggcac
    12421 ctcccggagc aagccgtgcc gggcggtgtg cgacccggtg atcacctggt cgatggcgcc
    12481 cagcgccgtt ggaccgtcct ggtcgtctct cccactgcca gtgggcggat catcgcctgg
    12541 acccgcgatt ggtcggcggc gttttccact gggcggactg ttgacgtaga acgcgccgag
    12601 atcacacaag gcgccagcgg cgaggcaatt ccccgctgga ccacggcggc tgcgggcgtg
    12661 ccggcggagt tcgtttcctg tcactgtgac cgatcggaga ccggcaccga tcgcttgcct
    12721 gtgtatcggg ttctggttct ttgggatggc ccccagggcc gctaccgact taagaccgat
    12781 gatggtcagt actacagtgt cttgaaagtc gagcgtccgg cactccttgg ccatcccacc
    12841 gttttctacg tctcgcccgc accggcaaac catcccacgt aaatgccatg ttgaaccgtt
    12901 tttctcccaa ggaggaccta cgaccatgat caccttggaa caggccctgc gcacccgctg
    12961 gcaagatgac cccaccttgg ccgcgctgct tcctgtcaac tatgtgtgga ccaattggtc
    13021 ggatgtggcc caggtgccgc gggccgaaat cacggccgct ggcgaagatc gggtttggct
    13081 cacctctcgt gcggaagtag ccgaacgggt ccgcgtcacc gtggaggtct ggcataactc
    13141 gttcgagaaa cttcgctcga ccatcaatcg gatcaaagag ctattcgacc gcgcatcgtt
    13201 cgacttgggc gacaatgccc gcatcttgcg actcacctat cagcgggatg acatggcccg
    13261 gcaaggttat gggctgtggc gaggggaaat cacttttgac ggtttggccc ttcgccccat
    13321 tgtggcctga tatgtgggaa gaacgccgag ctttcatctc gcgacaccaa taatcttgga
    13381 ggaactatga caaccgtctt gcgaagcacg cttttggcca aactgggatg gacctggcag
    13441 gatgagcagg atcgcgccgt ggtggccgac agcaatcaat ggacttggcg aaccgccctt
    13501 aacgagggaa cccaacctgg ggaggcaaat gccatttggt acgccgtggg caagaccttg
    13561 gcttccgggg caagcaccga atggttgctc gacgccctct cgcgacccct ttttggcaag
    13621 accatcgtcc aaggcttctc cagtgtgaag gtcctgtggc tggccaatcg cggtgagagc
    13681 accggtacgc tagtcctggg cggggcaagt agcaaccctt ggcccggtcc gttgagttca
    13741 ggaagcagca ccctgtgcgt ggcccccggc ggtgtcctct tcgctgtcca tcctgccgcc
    13801 ggctggggtg tttcatcctc ggccaaggca atcaaacttc tggccgaggc cggaagtgtc
    13861 acctacgaca tcattcttct cggtgtggcc agctaggtcc gcgccgatgc gagtgtcttc
    13921 cgccgattga tattcaaaca cggtggtcgt cgaagattcc ttggtcattc cctcgaggcc
    13981 ccatcctgga gagcttccat gcctgtgacg ttccgcgaac ttgctggttc acctgaagag
    14041 acctacaccc tggagggctt tcgcgcacgg cggaccttcc tggtcccttg ggaagaacgt
    14101 catgcctttg cggcggaaat ccttggcagc ggtggcgacc acggtgggcg gccctggtcg
    14161 cgctaccccg gcaaggagag tgtctttgcc gtggccattc gattctcccc cgccgatccc
    14221 gaagcccttc gcaccctcac ggaggaaagc cccgatgtgc aggaagtgct ggccgagtac
    14281 tccggctcct ttgcccaggc catcgtggaa tacactacca tcacgcctga agagcgtgag
    14341 gatacgccca gcccaccgct gcaaacgcat ctctcctatc ggatggaata tgggctgatg
    14401 gaacggcctt tattggcgtc gggcttcttc gccgaagatg atccccaaac acctctggcc
    14461 gccgaacttc ctctgacgca catcatcccc tacaccgatc actatttggt gtggcagcaa
    14521 gtgattaatc ctccctggca agccatccgc gaattacagg ggacggtcaa cgaggaggtc
    14581 ttcctcaatg ccaccccggg gacgctcctc ttcctcgggg ctaccaccaa taagctgttc
    14641 cgcggaagct tcgacgaggg cgtttccccc ttctgctggg aaatccgata tcactttcga
    14701 gagctggcca tcaagcacgg tggcaccgtc tatgggtgga accacctcca tcgagatcag
    14761 ccggctggat acgtggccat tgccgatccc gaaggcccgc tctacgacag cggcgacttc
    14821 tcccgcttgt ttcttcccga agtgtgaatt gcccgagcca acatgatgat ccacactgcg
    14881 gcatataagc catggtttgc cccacgtttg tccggcacgt atcccagtga tggcactaag
    14941 agcctatccc ataatcttcc ctcatgcgtt cggcaggaga ggggtcggag gtaagcgtgg
    15001 gtcgaattgc tgccggtttc ctcgatcgat tccgacgttc gcttgccctc accccaaccc
    15061 tctcccggag ggagaggggg actttttggg ataggcgctt aacgccggtg agggctccga
    15121 gttgcctgcc gcgtggatcg ttgatcagag gtcaaccatt tgattctcgc cgtgtcttta
    15181 gcggctcaat accgcaggag gcgtgacttg tgtggtcttt cccgcagacc tcgcccacct
    15241 tggccacggg gcgcactgat tcgctcccat tggctgatca cctcctgcac gaactgatag
    15301 tcccgggtac gtcgcaacca acgggccatc gccgccagat gagcatcgaa ggacgtccgc
    15361 aagaccagat cgggctcata gccggggccc agtcgcggga agcgggccag ggcaaaaagg
    15421 acggccaaga gaaacagatg caaaacgatc acattcagcg gatacaccgt cagaaaccgg
    15481 tagggtgaag gttcctccgt cggaccggtc accacctgat agccggggcg cagttcgaga
    15541 aagcaaaccc gtttcggccc agaggtgccc gattcatcaa tggcctggac caaccgcctt
    15601 gcaagtttgc gatgctctcg attcaccaac atcatgttaa ggagaaacga cccgttattg
    15661 acgagaagga tctgggatga gcctcgtggc aaacgggcaa ccagcacatg tttttccgag
    15721 cggatcagga cctcggcatc ttgggccgga atcatctgcg agcggagttc gatttccaag
    15781 tcctgcggat tgaccggaac ccgccattga gggtgcatct ccaagctctt gggacgaacg
    15841 acatccggga ggggtttgaa cgaaaaccac gcgcactggc cctcgacatt cgagatgagt
    15901 tgcaagaatt gttcctccag agcctgtcgc ctggcaatct ccttttgacg cacgggatca
    15961 tactgatcag gaaaccgctc caggagcttt ttccagtaaa gggatgtggc ctcaaagtgg
    16021 cggcccacat aaatcagcgt ccgcccgggt ttggccttga gccaatcctc caaccaggtg
    16081 acgacacttt cgtgggggac attcggatcc gcggcaaacc agattaggca atcggcctgc
    16141 tctttaagca tcggacttaa gtgtgtgctt cggctcaccg agtgccccga ccgacgcacc
    16201 atctccgcca acacccacgt gccgttgatg cttttccggc ccagccaccc catcgtgccg
    16261 tatgtgacat caacctcggg cgcaggccag agatagacag ccaacgcgat gaccaagacc
    16321 gaaacgatga ccccggcgag aatccaaaat aggggctttc ggaattccgc ctggggcgaa
    16381 gcactcatgc cgcgatctcc ttcaaccact gttgaaactg cggcaattct cgacgacacg
    16441 cctcgtattg ctcggcactc agggaacgac cgccgaaata ggcctcttca aaggtcacga
    16501 tggtccgttc cagaagagga gcgaccggcg actcgcgtcc caactccctc aggtattgcc
    16561 ggttggtctt ccccttcatc aggcgaatca ggcgatgacg atccaactcg gctagttgat
    16621 aggcataaag ccagataatg gcccgccgat agtctcctgc cgctgctgcc cgctcactgg
    16681 cctgcaaaag atccgtctct tgcggggagt cttccggaac aaggggcaac tcgcgcaatt
    16741 gggcagccac ccgcgctgcc tcttccttgc ctccttggca gggggcccat cgccgccacg
    16801 cccagtagcc caaaatgacc atggcggcaa tggccaaaac gattaagatg attcccactg
    16861 ccgaccaagg gacacgggag cgagaaacct tggaaggctc aggcaactcc ctgggcttcc
    16921 gcacgcgggt aggcttgatg tcgtccttct ccgcatcata ccaagggaaa ttgccttggc
    16981 ggagggattg gcgggctagg tcttccgggg cctcggcaag cgttgcattt gccacaagcc
    17041 cataggcgaa caaaaggccg acagcgagga gtaaacccat tggcccgagg ccgatctttc
    17101 gacaagtgcc gcgttttaac cccatcgaca ttcggtcgtg tgcgtcttgc ggtccgagac
    17161 aacttggctt tttacagtgc ttcttcatta tgacgggaag aaaatcgtcg ctcaattgat
    17221 gccgcgactc agcattttcc tattgcaatg ggctatcctc gctaagcagc cccgttttac
    17281 gttgaggcgc atagcgcggc gatccgttac ccccttcttg ctacggtggc tcgggcttgc
    17341 tgggagtgct taccgtgggg ggctgctttg ccaacggaca accatcccgc taggcaacgg
    17401 tcgtccgctg ctgctgggga tcgtcatcat ccgcacggtg atctgacctc gctgccaaag
    17461 gtcaggccgc aaatcgtgaa agagacgcgt caatttctgc gtgccgtagc ccggcgtgtg
    17521 acatgtcatg agaaggaatc cgcggtccca atcgagcaag gcaaagaccg cttccagaag
    17581 tcccacaatg tccttttcca attgaaacga ctcaccccgc ggaccatggc cgtatgtcgg
    17641 cgggtcaaga atgatccccc aataccgtcg ctcgcggcgg acctcccggc gaagaaattt
    17701 gcgggcatct tcgacaatcc aatgaattgg cagattctcc aatccggaaa gcacggcatt
    17761 tcgttttgcc caggcgacca cattgcgggc agcatcgacg tggacaacct cggcccctgc
    17821 caaggccgcc gccagggtgc tcccgcctgt gtaagcgaag agattcaaca ccggccccac
    17881 cttctcactt tgggtaatat ggtgataaag ccagctccag ttgaccacct gttccggaaa
    17941 aagtcccacg tggcccgatt cattccgctt tagctccagg acaagcggcc cccagcggat
    18001 cgtccatcgc tccggcacgt ggcaacggtc aacccaccgg ccacgattac cacgagtgcg
    18061 ctcaaatcgg gcatgggcct gggaccagcg gtcggggtaa aggggcggac cttccgccgc
    18121 cgggctgggc cgatcgagga tcacactccc aaagcgttcc agccgccgac tgtcaccgaa
    18181 gtcgatcaac tcgtagtcat ccgtgacaaa gggctcgaag gacgggtttt ccgaagcacc
    18241 caacaccgtt ccgtccacga tcgttcctcg agctggccaa ttattcctcg ggcacgactt
    18301 tgtacacgac gtcgtgttct cgcgcatgct caacaacttt tatgccgacg gactgtccag
    18361 cctgggcctg tgtcaccgtt tgatgttcga tctgcataga agtcaccggc tgatagaagt
    18421 cactggtatg ccccttaatg tggatagtgt cgcccaccga gagcgttccg tgggtaattt
    18481 cgatggcggc cacacccaga tggctgaagt agtgcgtcac acgtccgacg gggatttcgg
    18541 ccatggcgaa ggctcctttc tggcaaaatg gcacccgttc cgtgtgaccg gattgaaggt
    18601 ccacccaaca accgcaatca cacgccacag atcgcccaaa cgcgaacaac gtcacatcca
    18661 aagccgctcc gcaacgcggg cagagaattg ccatggcaca aaccctttgg cttccagtat
    18721 ggcggatgtc atgacgaaag acaagcgcgt ggatcttggc aggtcaacgc cacagtggtc
    18781 acgacgtgcc acgccggcgg gcgtccctcc agatttgatc gagcgcggcg caccaacgtt
    18841 gagaattcca catgacgtga gccaggttgg ctcaataagg gcctatcgca taatctcccc
    18901 tcgcccgttc ggcgggagag gggtcggggg taagggtggc tcgaatcgct gccggttttc
    18961 tcaattgact ccgacggtcg ctcgccctca ccccaaccct ctcccggagg gagagggggc
    19021 ttggggtaga tcttaagaag gggaagactt gtgcttcgcc cgcactggga atggaacaca
    19081 tagagtagat caaattggaa cacacacggt ccatggggaa caagcggttt aggctggcgg
    19141 cttgtatccc agatcgtcca cggcgcgtca gatggcccaa ctcgcttgcg ataaggggct
    19201 aaggccaatt gccgcgtcaa aacgatatgg cgtcattccg gcgttttggc tacatttggc
    19261 acgttgcgtg ttggtcacaa ggtgcgccag catctggaaa atgggggatt tggcaagaat
    19321 agaaagaatt tcagcaacag aaaaagtttc aacgaggcca gcgtggattg ttttcgcttt
    19381 ttgcacacgg gcgagctttg gttggatcgg cctatccgcg ttcgcggggg tctgccaccc
    19441 tcgctgatgg cccgcgtgct tgaaggtccc cggcggacct gggaacgcat ctgccaactt
    19501 gccttgcgtg aggatgtcga tctggtggtg ctttccggca atttggtaga tggctcgcgt
    19561 gcctcgcccg ccgatctgga cgccgtcttg gccggttgtg agacgctcgc tcaaaacgac
    19621 attgctgcta tttggatggc cgggaaagcg gaagagcaag gcccctggcc ggccgagttg
    19681 gccctgccgg acacggtcca cgtattctcg ggagaggagg tccagcagcg gcaagtccgg
    19741 acacggaagg gcacgatcga tgtgcttgtc tggcctacct cctatccgtg ggaggccagc
    19801 cgcgaggcct tccttcccca tcagccgggc caattcgtgc tgggactagc tcgctgccag
    19861 tcgctggagg aattgcccaa tatcatcggc gtcagttatt gggccttagg tggtccagtg
    19921 ccacggacag actcattgcc ctccaccgtg gcgcacgctg cgggtccccc gctgattcga
    19981 gatgccgacg atcccgcgct ccccaccgtg accatcgccg agtggaatat taaccgccgg
    20041 atcgcccttc gcgaggtttc tgtggcggca atccgttacg cgacggtggt gttgctgatt
    20101 tcgccggaga cacaatgggc gggcttgtct tcccagttgg ttcaagaatt ggaatcccgt
    20161 cgggccagca gcgatgccga ttggctcatt gccatcaaac ttcaagggcc ggtcgaggcc
    20221 ctcattcgct ggaagcggga agggttggac gctcaacttt tggagtttgt tcagagccgc
    20281 tacctccaag agccgccgta cctttggccg acggatgtcg tctgggaccc cgtcgatcta
    20341 ggaccagacg agtggcttaa ggaaacgagc tttcgtgggg agtttctccg ccaggtgcat
    20401 gcgatttacg aaaaccttgt cataggacag acggacgcgg tggccgcttt ggcggggccg
    20461 atttcgccag gccaagccgt cgatgccatc gttccgatat gggaggaaat acttcgccag
    20521 gcggcgtttg agggactgga ggccctgtcc catgtgagtg cctcagtaaa gggagaagcg
    20581 tcggcgtgct cctgaagcgt ttagccatcg accgatttgg gatttggcac aactgggagg
    20641 tggaagacat ccccaggggt ttggcggtct tttttggacc aaacgaaacc ggcaaatcga
    20701 caatcctgga attcgtgcgg gggatgtttt tcggcttggc ggcgcggtcc cgctttctcc
    20761 tgggcgaaca aggtgacatt gggggaaccc tggtcctcga gcatctcggc cgcgaagtcc
    20821 gcctctcgcg acactggtcc caggatgagg gagagagggt ccaggttgtc gtggacggcc
    20881 aacccgtctt gcctgaggaa ctcatgcggg tcctttgccc cgtcgatgag gccacattta
    20941 acgcagtatt tgccttgggg ctggaagacc tcgcttatct tcgtacccta gaggaaggag
    21001 aaaccgccaa gctcctctat gaacttagtc tcggcgcgga tcgggcggtc ctatggcgag
    21061 tcctcaccaa tcttcaccag cggcttgccc aggaccagga cgacacggaa ctcaaagagc
    21121 tggaagcgga acgtgcgcgg ctcgttcgta ctaccgagga agcggggctg gagacacggc
    21181 aatacgtgcg gtggcgggtc acgcagcgga agctccaggc ggaacgacag accgtcatgc
    21241 aacggcttga ctcccagcaa cgggagcaac agcgctgcga ggcggttctt gatcttctcc
    21301 caaagtggca gcaatacagg gaaatgcggc tgcaatggaa acggctgggg ccgtcgcgac
    21361 ggatttcaga aaaggcctta cgaaagctcg accgttggaa agaacgaatg actgcggcca
    21421 agcacaacct tgccgaggcc cgcgaggcac ttggccagta cgatgaggcc ctcgctggct
    21481 tgccggttga tcccgtggtt cttgagcatg cggccgagat tgaggccctc cgtctgcacc
    21541 gcgatgagat ggtcgcctgc aaggagcgac tagaagaact gaaccgacga caacaagaac
    21601 tccttcaggc tcgatcggca ttgtgggaag gtttggcaag cgttacccag atcgggaaca
    21661 tctccacgcc gaatgatggc ttgccgcaag cggatcagcg gggcaccgcg caggattggt
    21721 ccgccctccg tgggtggatc aggcggtggc aaaaagcggt cctggaaagg gatcgactgg
    21781 ccgaagagga tcagcggcaa cgcgccgcgc aaaacgcact caaggagcag gtggaagccg
    21841 cgctgcaaaa acacggtgtg gccgacgccc aggtcgcctt gtcacaatac agcgagcagg
    21901 tgaccggcct gcgacgggtc cgacaactcg ctgaacagca ggccgccgta aaacagcggg
    21961 tggaacaagt caccacggaa tatcatcggc aggtggccca atcgcttccc tcgtggcaga
    22021 catgggccgg tgtggggagc ctctttattc ccgggcttac gctggtcttc ctgtcgctat
    22081 tggcccttct cggtggacct ggagcgggaa acctggggtt ggtcacgctg ctcctcggcg
    22141 cggcgatgac gggcgggggt attggactga agttttacca cgaacatcgc cagcgggtta
    22201 acctggagaa tttgaaaaag gatttggaga ccctaactac cgagttgcag tccgtatctc
    22261 aggaatacca acggctggcc gctgagcaaa atgtctctca ggcggacctg gatcaactga
    22321 tctcccaact ggaagggcgc atccgtgagc tggaacccgt cgccgtcctt caggcgaaat
    22381 ttgccgagat gacccaaccg gggcaaacac accgcgagac cctccacagg ctggaacaaa
    22441 aagagcgggc aatccgtgcc aagtttaacg aattggccca tcgtttgggt ttaccttcgg
    22501 cggacgatgt gacccaagcg ttgcaaattt gggaagtggg gcatcggctg cgagccctcg
    22561 atcgccgcct gacagttgtt acggcaaaac tgaagaagga ggaggaacgc ctcgctgcct
    22621 ggcaaaaacg ggtcttacga ctggcccgac tttgtgggcg ctcggatgag acggacgacg
    22681 tggtccaggt cctcgatggc cttgtgcgtc attatgaaat ccatttggaa aaccagcgcc
    22741 gtcggcaaga cctgcttcgg acggccaaac agaccaaaca gaaggttcac cgcgcgaaga
    22801 gacgcctcaa gaagattgcc cttcgttatc gggcgacact tcggcaattg ggcgtgcgga
    22861 acccccaaga ggccacggcc cttcgccaaa cttgggagca ggcccaaacc ctgcggcgac
    22921 aggccggcga aatccatcgc gaattgaagg ccgcccttgc tgccgctggg attgccgaag
    22981 atgtcccgct tgctcgcctc tttcaagaga caaaggccaa acatgccgag ctgatccagg
    23041 ccatggaggc tactaaggaa gaactccatg ccctggagga aaagcaggcg cacgtagagg
    23101 cggaacttgc cagacttgtc caatcgcgcg acgtggatca ggccaagttg gctctggccg
    23161 ccttggaaca ccaaatcgtc cgccgtgtcc gaagacaaca aacttctcgc ctccttgcgg
    23221 aactgctgga acgagttcgc cagcattatg aactggagaa ccagcccgag acgttgcgaa
    23281 gggcctctgt ctttctcgcc caaatgaccg agggacgtta cgtacgggtt tggacgccta
    23341 tcagcgaacg cgtccttctc gtggaagatg ccactggcaa gaattggcgt gtcgatgagc
    23401 tgtcgcgggg aacgcgggag caactcttcc tcagctttcg cctggccatt gtggagcgca
    23461 gcgccgagca aggaattcgg ttgccgctgg ttctggatga tgttctggtc aatttcgatt
    23521 ttcaacgtgc ggcggcggcc tgtcgcacat tgcgggcgtt tgccgaggca tctcaccagg
    23581 tccttctttt aacctgtcat gatcacattg cgcgattgtg caccgaaatg tcggtccccg
    23641 tcgcccacct cgaaacaacc ggcggtggca aggctactcc gccttttttt cgcggatact
    23701 tccctcccct gaagacggct gacacgccga tcgctcccac aacacctgaa acgccccgtt
    23761 taagcggttc acaggaggaa aaaacagttg ccgacggtcg agagaaagtg ttgtcacagg
    23821 agccatcgcg gtcacgccgg cggaggaagc agacccctaa cggaaaagcg gtcgaaaccg
    23881 aaaaggtgct cggcgatcac aagcaacctg gcgaaatggc ttccccttcc gccggtcagg
    23941 tcttcttcca aagcaactgt acctggttga cggaggccga accctacgcc gatgttaacg
    24001 aggagggaaa tccccagggt gaggcgaaag gggatcgctc gtcggcagaa ccagtgggga
    24061 atcatcgagc aaagcagcgc cgggcgcgtg cagcgtgatg acgtttttgc gacaatacct
    24121 tcacggtcgc atctgtgcta cgcggagctc tgggcccaaa caacgcccga ctcatcggcg
    24181 gcctcggccc gaccaatttg ccaacacggc acccccaggt cttccaattg cttgcggata
    24241 ctggcagcgt aatagtcgct gaccacgagg atcataccca agccgaggtt aaagacgttc
    24301 tccatttcgg catcgtcagt ctcgcccaat ttttggatcc agcggaagat gggcggcact
    24361 ggccaggcgt cccgatcgat gacgattcgc acgcccggcg ggacgattct ggcaagattt
    24421 tcccgaatcc ccccgcccgt gatatgggca atcccatgaa ccacgctctt cacccgatag
    24481 taccgtaaca ccgagcgaat gggacggacg taaatccgcg tgggaaccaa aagggcctca
    24541 cccacagtgg tgcccaattc cggcacatga tcggttaccc gcagcccggc aagatcaaag
    24601 actgtctttc tcacgagact gaacccattc gagtgcaatc cactggacgc cagaccgagg
    24661 actgcgtcgc ctggcgtgat ggcctgaccc gtgataatcg cccgggcctc agccaccccc
    24721 acgcaaaagc ccgccagatc aaaatcaccc ggctggtaca tgtccggcag gatcgccgtt
    24781 tccccgccga gcagggcgca gtcagcctcg cggcatccat tgacgatccc ctccaggagt
    24841 ttttccagga gtggcggatc gtccttgggc atggccacat agtcgagaaa gaagagcggt
    24901 tcggcgccgc aacagagggc gtcgttgacg ctcatggcca ccaagtcaat ccccacggtg
    24961 ttaaagatcc ccatcatggc ggccaccttt aatttggtcc ccacgccgtc ggtgcaggcc
    25021 accaggacgg ggtctttgta gtttcgggca aaaaggggcc aggggaagtc cagccgaaag
    25081 agtcctgcga aaccaaacgg caaattgatg acgcgaggcg tgacggttct ttccaacaat
    25141 tgggggatac gcgccatcgc ctggcggtag agatcgagat cgacgcctgc tgacttgtag
    25201 gtgacttttc ccatgggatt gacttcggat agacggcaga ttgaggacag tcaatacaaa
    25261 aaattgtaac gggctttcgc cgatgtgact atggaaccgc gtgccacgct gtcgctccga
    25321 ggatgagccg catcgtgata agggcctttc tcataatctc ccctcacctg ttcggcggaa
    25381 gaggagtcgg gggtgagggg gctcgaatct ctgccggttt tctcaatcga ttccgaggtt
    25441 cgcttgccct caccccaacc ctctcccgga gggaaagggg gcttttcgct cagtgcccat
    25501 cgtcgtgagc ccttatcgca agatgggggc tcgtcgtgct agaacatcgc gccacatcgg
    25561 gaaaaaacac gccaccttac ggtggggagc cggggttctt gcgataggag ctctcggggt
    25621 aggaataaaa actggtcttg agtgatttgc catcattttg ataacctttg ctccaaaatg
    25681 gccgagaggt gcgtgtcatc ccccgaaggg attgcacggg tgaatggtgt catgaggcgg
    25741 gtcaagttgg gcctttcccg attccaaaaa catcggaccc atgccccgtc tgttggcgaa
    25801 ctgggccaag gatgtcgccc taagtagcat ggatcaacgg taatggagtc ctagacggtg
    25861 tatttcggcc gtaccagaaa gcgagcctcg tttgtggcgg tgttcgcccg gctttggccg
    25921 gtgggcctcc ttctggtcgg taccatcgtg ggcaacggct gtgcgaccaa tcctgcaacg
    25981 gcggcggccc aaaatcaggc agcccagcag caagcggccc tggcccggca gatcgaggaa
    26041 taccaaacgc gatcgaccgc cctcgatcgt gacaatcagc agttacagca gttgctcgcc
    26101 caggcttatc aaaagaacaa acttcttgaa gatcaggtcg gactgttaca aaagcaattg
    26161 gcggacatga acaaccaaat cgctcggctg caacaggaga aggccgagca acagaagcag
    26221 gtccaaactt tgacggcctc gttacagcgg tccggaagcg tgaccattca gcccaacaat
    26281 agtcttctgc aaacccttcc ggccatcaac attgcgggtg tccaggtgcg gcgagatggc
    26341 gatgtcattc gtgtggagct tcccgcggac caactgttcg agacgagcac gttccagttc
    26401 cggccggggg ctgtgcagat gatcacatct gtggccgcgg agattgcccg actctacccg
    26461 gagcaaatca tcggcgtgga aggttttacc gacaccgatc cagttccccc gggattgtgg
    26521 cggaaccaca cgcagatgtc gatctcctgg gccgcagcgg tgcacgatgt cctggtcacc
    26581 caaacggcct tgcgcccgga gcagcttttc gtggccggtc acggatcggc ctatcctatc
    26641 atgtctaacg gcacggcggc aggacgggca cgcaatcgcc gcgtggaact ggttatctat
    26701 cccgagaaat accgttcccc cggccgttcg tgacatcgct agggccggat gatcgcccgc
    26761 aggtcctcga aaaactcggg atacgtcttg gccgtgcaac cgggatcgag gatttttatc
    26821 ccgggaatgc gtaatccagc gatggcgaag ctcatggcca tgcggtgatc ctggtaggta
    26881 tggatttccg ccggatgata ggaggcgggg atgattctga gtccgtcctc gaattcttcc
    26941 acctgggccc ccaggcgtcg cagttcaagg gccagggcgt ggatgcggtc ggtctctttg
    27001 tggcggatgt gctttacccc gcgaatgagc gtcggcccct cagcgaagag ggccacgacg
    27061 gcaagagtag gcactgtatc gctgatggcg ttcatatcca cggagatgcc gcggaggggg
    27121 cggccttcca cagtgatctg gtcatcgccc attgtcacct cgcagcccat cgcggccagg
    27181 caatcgacaa agcggacatc accctgaagg ctattctcgc ccaggccggt tacggtgatc
    27241 cggccctggg tgacggccgc ggcggcgaag aagtacgtgg ctgccgaggc atcgggctca
    27301 atggcgtagg ttcggccgcg atacatttga ccagatcgca cggaaagccg ctgaaaggcc
    27361 tcattttcgc cggtgacccc aaaggccttc atcacggcga gcgtcatttc cacgtagggc
    27421 ttcgagacca aatcaccccg cacgaggagc gtcacatcgc ggcgggcata aggggcggcc
    27481 atcaacagac cactgaggaa ttgactagag atttccccag aaatctccgt ctctcctccc
    27541 ggcagaccat gggcttcgac aatgacgggc ggacatcccg tgggaagctc tgttcgcacg
    27601 ttagccccca gcggattaag ggcatccaca aggtcctgga tgggccgttc tctcatgcgg
    27661 ggcgtcccat ccagtcgaaa ttgaccctct cccaaggtgc agatagccgt caaaaagcga
    27721 aggctggtgc cactgttggc gagaaagaga tcggcttggc gagcggggat cacgccgcgg
    27781 gttccttcca ccgcgatttc ccgctgcttg gtatcgtggc ggaccgctac gcctaagctc
    27841 tgcaatgccc gcaccatcac ctgcgtatct tcactatcca acgccccttg caagagggac
    27901 ggcccgtcgg ccaacgccgc acagatcagc gcacggttgg tgatactctt ggagcccggt
    27961 ggctgaacgg tggcgttaac cggtccagac aaggggattt cgacgaaatc gcttgcttcg
    28021 gccatcatgc tttctctagc aatgtttggt caacaatcgt tgaagatccc gggagatctg
    28081 ttgccaaaaa ccaatcggca tccgagaaca ttgcccagta gcccgtgtct ttattctttt
    28141 ccggaatccc ccaatcggaa agcgtataag aactgatttg tccggaaata gagttgccgt
    28201 ccatcgctgg cgggtgaggc cagcacgcct gattcgaggt cccattgccc ggcgaggctg
    28261 ggaagccccg cgtcatccag tgccatgacg aacaccgtac cctcgtaatt gacggcgtag
    28321 atgtatcggc caaccaaaag cggagaggcc caaaagctac cccctaggcg gacttgccgc
    28381 agaatgtcgc cggtctttcc atcagcacag gcgaagatat tgggggactt gaccaggtat
    28441 atcttgttgt tccagacaag agggctggta tttccacagc ggaggcgatc ggaaatccaa
    28501 agttgctcga acctcgccgg ggaagaagat agccgcaagg cgtggatgcc cgccgcgggc
    28561 aaggcaacca aatcaccata agccgctgcg gaggatacgg tgtcgcacca atgatcatag
    28621 gcaaaggcca cctcgccggt gtaagggcag acggcttgaa agtcctttcg tgattggaga
    28681 aggacgaaag tctcaggggt tcgctcggag ggcgatgggc agcgcacgac caacggcgtg
    28741 caccaaaggg cttgcttcgg acgatcaatc agccaaacgg tctgtcctgt ctttagatcc
    28801 agtccgcaaa caatggcatc gaggaggctt tctgcctgga cgataaccac atcaccctca
    28861 atcactggag aggaggccat tcccacatca ttgcggaggt aggggcgttc aaatcccagc
    28921 ccacggaacc agaggggacg tccctccata tcgaggcaga cgaggtcatt agacgaaaag
    28981 agggccacaa tccgccgacc atctgagacc ggcgtcgagg ccgccacacc gccgaaggga
    29041 ttgcacacgg tggaaccagt cgcccaaaag cgaaaatccc actcctgctt gcccgttttt
    29101 gcatcgaagc agaggacgtg caactcgttt tgacgataac ccacagcagc cgtgacaatc
    29161 accttatcct tcaccacgat gggcgaggac ggaccgcggc ccggcagtgg ggccttccac
    29221 aaaagttggt tctcggggga gagtaccggc gggatgtctt cacccccgca gagcacgccg
    29281 gccgctagac cgccacgaaa cgccggccag tcctccccct gggccaattc ccacgtgcca
    29341 cagcaggcgc cgaccagcaa ggccgcaagg tagatatgca tccgaacctt gatcattgcg
    29401 gaaccttccc accatgttca aaaagcaggc cgctcaatcg ggttgacgac caagtccctt
    29461 gccgagctcg tcgcagaccc gcagccgaaa cgtccaatat tgtgcccaat cttgcgtctg
    29521 ttcgttctga cagactttga tcatgatcca attgacgcca ggttgcaggc ggcagaccac
    29581 gcggtattga tcgtcctcaa aaccggagtg atacacatgg aaggccccca agggttggcc
    29641 gttgcaccag attttgatgg cgttcggcgt tgccgcgcga atttccgcgg cttgttccgt
    29701 ctgagaaacg agggcggcta ccgcgtaggc aacaacaccc ttttcctcgc cgaggacttt
    29761 gttgagatca agggttccat gttcgtcagg ggtcgtcact tttttccagg ttactggccc
    29821 atgttttccc tgatacgtgg cggagaagtc tggcggggat tgcccctccg gcggatagat
    29881 ggcgtcaaaa ccaacgtctt gctgattatc aaacggcccg acaatccacc acgtgcgcag
    29941 gtatccgtaa tgaccggcca ggtccacgga cacgccccgt ttgcgcagca tttcggcgag
    30001 gttttcaact tgatcttggt cccgggcagc gtcaaacagt tgtttgagct tccgtacaat
    30061 ctcgacgttc ttgggatcat ttaccggaac gttttgaata tcacctagcg cttgaagctc
    30121 cgcctcaatg gccgcacgtc gcaactcgct ggcgggatca ttgaggaatc ggatcaggat
    30181 ctgccgagcc tgggcggggt cttgccgagc gagccattgg tacgcccacc agcgaccttg
    30241 cggaggattt tgcgtatcct cgagcagttt aaggatcact tcctgcgaga gttgtcgccc
    30301 agcgccgtgt tccctcgcta caatctgatc cgccgccgaa aggagccaat tgcgggatgc
    30361 gggatcagcc tggcgggcag ccacaaataa gggccccaag gcatcggggc cggcgcttac
    30421 caaacgcttc caggcctccg agacgtcctc aggcttcgtg gtggactgac cgctgcttaa
    30481 taggatagcg atgtcccctg ggatatcgcc tgtcgcatat tgcgcagcga gtaatcccaa
    30541 aaccaacccc agtgcaaata ccacgtcctg ccagggaacg ggacgagaca gtttgattcg
    30601 ggtcatggga tgcctctctg tgaacctcgg caacatggga tcacagacac catttatcgg
    30661 gcaagatggt ttctttgggg atcacgacca cgccgtcgcg aatcatgcaa tactggtttt
    30721 ccggcatttc ggtcagcccc gttgtgttga cgatgcgggc attcttgccg atgcggcaat
    30781 tcttatccac gatggcattc tcaatgtaag agccttcgcc gatgatcatg ggaggtttgc
    30841 catcgcgggt ctgctcaaga tcgtagaagt cgttgcccat gatgatcgag ttgcgaatga
    30901 cacagttgcg accgatgacg ctccgcaggc cgatgatgct attctcaatg accgacccgc
    30961 tgccaatgat acagccatcg gcgaccaaac tagactgaat ggtggccgac tcaatgcggg
    31021 atgggggcaa aaacctcgct cgtgtgtaaa tgggggcgtt ggggtgttcc agcgaaaacg
    31081 cggcgttggg ttttgtcagc gcgaggttcg cctcgaaaaa ggctccaatc gttccaatat
    31141 cttcccaata accgtcgaac agataaacga agacccgccg agtgcgaatc gacgccggaa
    31201 atacctcttt tccgaaatcg cggtaatccg tcatttcgag gacatcgacc agtgtcttgc
    31261 gggaaaagag gtaaatcccc atattggcca ggtattcacg cccctccgcc tggatgccga
    31321 attggctcag ccaatcggga tcggtttgca tgtaccgcaa ttcttcttcg gtttgaggtt
    31381 tttcgaggaa gccgaggacc tggccgtgct cgtcaatcca catgatcccc agacttgatg
    31441 cggcgcgttt catcacgggc agggcggcga tcgtcacatc tgcgcccttt ttctcgtggg
    31501 ccgcaatgag ctcccggaag tccatgcgat acagttgatc gcccgaaaga attagcacat
    31561 agtccacatc atcccgattg atatacgcaa gattctgccg gacggcatcc gccgttccct
    31621 gataccaatc ggagctggtc aatgtctgct gggccgccag gatttccacg aatccccgat
    31681 ggaagacgtc gaaattgtac gtcgctcgga tatggcgatg gagacttgcc gaattgaatt
    31741 gggtgaggac ataaatgcga ctcaagtcgc tgttgatgca attggaaaga gggatgtcaa
    31801 tgagccgata tttcccggca atcggtaccg ccggtttgga acgaaacttc gtcagtggat
    31861 agagtcgtgt gccacgacca cctcccagaa cgatggtcag gacgttttgc atagacttcc
    31921 cttacacgct agaacccagt ctcagctctt tgacgccttc tgcccatact agccgaaatc
    31981 attcccgatg caagtcttgg c
//
LOCUS       DAOSUT010000026        31544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_980_length_31544_cov_9.968687, whole genome shotgun sequence.
ACCESSION   DAOSUT010000026 DAOSUT010000000
VERSION     DAOSUT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 31544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31544
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_31544_cov_9.968687"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLD05_03820"
     CDS             <1..204
                     /locus_tag="PLD05_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76589.1"
                     /translation="ELITNLPSQTKNVETKHHRKLADSLQRQVDYSGKGQALAKILHG
                     FTCVWPVFAAQPAATHKTGLHFD"
     gene            582..1592
                     /locus_tag="PLD05_03825"
     CDS             582..1592
                     /locus_tag="PLD05_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOP76590.1"
                     /translation="MKTIGVDSAKGHRVLAALAASCFVWGLLADVTRAQGTNGAEGPF
                     TVEKTEHGVSVKYDGKLWANYVADGEFKPYVWPIIGPTDQPVTREWPMGEGLNERHDH
                     PHQKSMWFTHGDVNKTDFWAEGPNRGRIVHREFVQVSGGKNAVIVTRNDWLLPDGTKT
                     LEDTRKLIFSRSGDHRIIDFVIRLTATADSVTFGDTKEGTFGLRVAESMAVDSKKGGQ
                     IMNSEGQTDAQAWGKKAKWVDYSGPVGDQIVGIAVLNHPTSFRFPTHWHVRTYGLFAA
                     NPFGWHDFVGRSDVSGAYTLGKGQSITFAYRIILHKGRAEDARIAQAFEEYAAQPVGD
                     LP"
     gene            1869..>2338
                     /locus_tag="PLD05_03830"
     CDS             1869..>2338
                     /locus_tag="PLD05_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76591.1"
                     /translation="MSQIVAKSPNGLPGVPGGLLNNFAQWVADIGARTMEWTIGLGSI
                     SLFFGRTLRWLVTKRPRRETILWNFYQIGVLSLPVVALTGTFVGMVLAVQSYAQFKML
                     NLETRLGAVINMSLVRELGPVLAATMLAGRVGSAMAAELGTMRVTEQIDALSVMG"
     assembly_gap    2339..2438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2442..2615
                     /locus_tag="PLD05_03835"
     CDS             2442..2615
                     /locus_tag="PLD05_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76592.1"
                     /translation="MGCHHGFHCDPGAEGVGRAATYAFVHSFVMILLLDLILGIGLDA
                     LYNVLFPQGPRLV"
     gene            2728..3612
                     /locus_tag="PLD05_03840"
     CDS             2728..3612
                     /locus_tag="PLD05_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76593.1"
                     /translation="MIAQATTEGTISSPSSKDPVLLEVEQLTVAFGRQVVLRGINVQI
                     RRGETVAILGESGCGKTVFLKTLVALQRPTKGRVLFDGRDLFQLRPHELAEQRIRMGF
                     VFQQAALFDSMTIGENIAFPLRQHTRLTEDEIYEVVKARLLEVGLPENVLRKKPGEIS
                     GGMRKRVGLARALALEPELMLYDEPTTGLDPIMSDVINELILRTRRQHPVTSVIVTHD
                     MRTATKVADRIIMLYPLTRLKPGESQILYDGPASEIESAADDRVIQFVRGEARERLME
                     MAREESENAVEIGGAAHG"
     gene            3605..4783
                     /locus_tag="PLD05_03845"
     CDS             3605..4783
                     /locus_tag="PLD05_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOP76594.1"
                     /translation="MDERLMQFRIGVMVLASILIAAILIVTFGEWRWPFPAEYTVYIR
                     FPEAPGVAERSPLRKSGIVIGYVRSVELTPEGNVLVTARVERKYTLRHNEVCRLNRAL
                     LGDSILEFSLSPDPNAPKDIVKDGDVIKGVVPPDPIIVLTNLQERLVTAINSVNETSQ
                     ALGDTVRSVNRILTENEQQLKELLSNANQTVTAAQQTVTNANEIIGDPELKGRLKEAL
                     SRVPQMVDEIRATLDETNQTIRTARANLENLRGVTEPLAQNGPAIVQRLDNSLTNLER
                     VSAQLAEFSEALNREDTTLGKLVRDPTLYQKLDRTMTNIQSLTQDLRPVISDLRVFSD
                     KIARHPEMLGVRGALERQAGTKGLPNLPSFTNQGQVETYPIEVPASNQRFSVLPGTLR
                     "
     gene            complement(4892..5782)
                     /locus_tag="PLD05_03850"
     CDS             complement(4892..5782)
                     /locus_tag="PLD05_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76595.1"
                     /translation="MDPVEREEKLRTVLVALDRKEFLRALSLADQWVTESPSDPVAYS
                     MRAYTLLNLQRSRDALEDARRARELSPDDVTVLSLFAQAAAAEHRLGWAQEAWEKAVA
                     QSGGSPQILREYARFMVHYRGPRLALEAAQRAQQADPKNSVGWSLLAAAQLRMHQYQE
                     AENSLRQALALDPNNVQAQSLMAKLLLLKGKQDQALALSQLLEDDAEARPIAEWIRRE
                     IKKRDLERILVERALDMSAHRPDPGVFLRFRLMWWIVAYIGALALALFLDSLIEGQVF
                     FIFAAVFFVVHVLIWLLLFH"
     gene            complement(5789..7132)
                     /locus_tag="PLD05_03855"
     CDS             complement(5789..7132)
                     /locus_tag="PLD05_03855"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOP76596.1"
                     /translation="MTISVFWKVCLVWGIILVTFYSGIGALDQRFASVSVAEEQASQP
                     RSAAPWSVLQRVLNNIFASEDAFPVLPQRLEESEASSAPVYEAGTYDRGELRIVNQVP
                     VLILRGKPEEIAIQEAKLTSDAIANLVIAPQEFLRQLNYPGGWEGLIARGRNFWRYVP
                     ENYRRELVTMAEAAGLNHDELFAINVLPDIYRSIACSSLIVLPERSATGKVLFGRNLD
                     FFSPRQLYRYTIVKVYESDGGGHRFVSIGFPGFVGCLTGMNDAGLCLAVHEVHFTNDG
                     SPICSPHGIPYAMMTRQIMEHCSNVEEAIAHLRKSPRTTLLNIALCDPKRAVVAEITP
                     KTVAVREAERGLLACTNHFCTPELKVFVISRRYGELLEAYNIPKIDVATVQKKLDDVN
                     LGFLSIQSVVFEPEDLVMHLSAGFPPVTKRPYHRLEMRTFLQSEAKASSPTISSR"
     gene            complement(7347..8759)
                     /locus_tag="PLD05_03860"
     CDS             complement(7347..8759)
                     /locus_tag="PLD05_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76597.1"
                     /translation="MKFRRQFFVIMAVMATGWFSAVVSRGEESLPYVRISPRDPRYFE
                     LSDGKPYIPIGLNMIRPPTDSLDGMEVWFSKLSAEGGNFVRIWLSSPFFDVEHERSGV
                     FDEERAKRIDGLLALAKEYGIRLKLCTEHFRHLGEGTQAWAAKPQHLIANGGPAKDTA
                     DFFLGEAGRAQYKRKLAWYAARYGSDPIIFGWELWNEMDAVRVNVWEPWTAEMLPELH
                     RLFPQNLAMQSLGSYDHENKRERYRRLCELPGNDVLQVHRYLDLGAAWKICHGPVAVF
                     AAEAIKDLRSFGIQKPILLAESGAVEPSHSGPFKLYEKDKDGIILHDVLFAPFFAGAA
                     GPGHIWHWDVYVDKNNLWWHFGRFAEVVRDLDPPAEHFQPVELEHPRLYVYALKGTGK
                     TLIWCRDRENAWQTELAEGKPPATLHGIKLELPPDFRVQESKTVRFYDPWKNAWEVGK
                     TESPLLLPDFRRSLVIRIEY"
     gene            complement(8780..10081)
                     /locus_tag="PLD05_03865"
     CDS             complement(8780..10081)
                     /locus_tag="PLD05_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76598.1"
                     /translation="MDTSLSRRMFLKSQAAVAAGAVAAGVLSAEETPPQGAPLRIGVI
                     GVGGRGTYLLRLLLEQGAEVPAVCDIRPARLEIAGKLIAEMRDGQRPATYGDTPTDYR
                     RMLERDDLEAVLIATPMQWHAVMAVDALRAGKHVLSEVAAAMTVQECWDLVNAVEETG
                     KIYMLAENCCFWRPVLAIGQMIAAGLFGQLTFAECGYVHDCRALAFEADGTLTWRGEI
                     KRDHRGNWYSTHSLGPVAKWLGINKGDRLVSLVAAETGRAAMRYYVEQRFPEGHPARQ
                     IDFRAGDSTTVLIKTAKEVLIDVRYDVMSARPHPTTVYYTIQGTKAVYDSRIDSVWIE
                     GRSKAKAWEPAGPYVEEFDNPLWKEHAEKAQKTGHSGGDYFVIQQFAEAVRKGGPVPV
                     DVYDAAAWSSVMPLSEQSLNEGGKPVEIPDFTRGKWEQIAS"
     gene            complement(10230..11372)
                     /locus_tag="PLD05_03870"
     CDS             complement(10230..11372)
                     /locus_tag="PLD05_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOP76599.1"
                     /translation="MRAIFLLGISFTLLPIFVHAADTPETIVAGLQAHNRALHIKDGW
                     IRDPFIVLAPDGYYYLTGTTALPGDARERNDILNTGLGPQSIVGWKVRLWKSPDLIHW
                     EYCGEPFSLKDGVWAEVQPERFRQVPESAWRLWAPELHWLGNRWALVHTSPSPVRASN
                     LSLTEGPEIKGPWKNPMGEKIGHRHDPSLFRDDDGTWWLVWGATEIAPLKPDFSDFAQ
                     QPIKIGPTEPDGTLGTMGHEGCFIKKFGRKYVLFGTGWSTRQMRKGSYNLYYATADRI
                     EGPYSVRRFVGRFLGHGTLFQDKAGHWWCTAFYNANVPPLSREEARGKDLSETAQTIN
                     PQGVTIVPIFIKTLPDGDVFIRAKDPDYANPGPDELQQFDYWEPQE"
     gene            11615..13186
                     /locus_tag="PLD05_03875"
     CDS             11615..13186
                     /locus_tag="PLD05_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP76600.1"
                     /translation="MNFWAMPSMKANKLALSWLAWMLTIVALVATSSATETSQQRRNI
                     LLICVDDLKPVLGCYGDPVAKTPHMDRLAARGMRFELAFCNQAVCAPSRNNLLLGSRS
                     TSIGIYGLSENFRRAVPDAVTMPQYFMKYGWRTEAVGKVFHTGHGNHDDAASWSVPSV
                     IEKVVEYVDPQNSAGGQLTREEAYFTNQILDRIGSLPRGAAWEKLDVADQAYADGRIA
                     DEGIKRLRAAKERRQKEGTPFFLALGFVKPHLPFTAPKKYWDLYSPTEFKLASYQEPP
                     KGAPPVANKRGGEILNYDPLTMENLKTDEIQRTLIHGYYAATSFADAQIGRVLDELDR
                     LGLAESTIVVLWGDNGFHLGDHGIWTKHTNYEQAVRIPLIIVAPGVTPPGSVTRQVAE
                     NVDIFPTLAELAGLPRPTGPQPIDGVSLVPVLRNPDTRVDDHAYHCFPRGAVMGRAIR
                     TERYRLVEWKKIGAPAETAEYELYDYQTDPEERENLIDKLPDVAAQLKKILAQYPEAA
                     IPVTQRRQGNPVVKP"
     gene            13254..15182
                     /locus_tag="PLD05_03880"
     CDS             13254..15182
                     /locus_tag="PLD05_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76601.1"
                     /translation="MACKVRSLLLVTILGIVPFVCLCPTLRAWANEGKPYLRPNIVYI
                     VSDELGYYELSCMGHPHFQTPNIDRLAAEGIRFTQALAGSSLCAPTRCCLMTGKHSGH
                     TSIRTNGGGTPLRPEETTIASVLKQVGYATGGFGKWGCGGRGSTGVPELHGFDIFFGY
                     YDQVHAHSYYPPYLIRNSEEVPLPGNRGLSEGTTYSHYLIVEEAKKFIRENKDRPFFC
                     YLPVTPPHGIHDIPETDPAWAIYRDKPWPLDARKYAAMVNMVDRHVGEIVDLLKELGL
                     REKTIIFVSGDNGGAEYFRDADHPRGFHAPNVNPVTGVQFRGGKGNLYEGGLRVPMIV
                     CWPGRIKPGRVSDLLWYFPDVLPTLAEIAGATPPPDIDGISIVPELLGEEVVGRKQSQ
                     HDYLYWELSGQIAVRRGTWKAIRPGAGKPWELYDLSKDISETMDLSAEHPKILKELIA
                     LAEKAHQPAVEGDFLQPELNEKDRRAKFGDAVPPEKINVALVQNMPQAGLIPRKNYRI
                     VSCSSESISNGRLAQHAIDGNPRTWWHTQFSPEVKRPPHELVIDLGSEHIIRGVRYLA
                     RQDATWNGTIKDCDLTVSATPEFPDEPTVKLQFSKTRECQERLFPEVRGRYVRLRIFS
                     EVNGGPWASIAELGFVGE"
     gene            complement(15200..15940)
                     /locus_tag="PLD05_03885"
     CDS             complement(15200..15940)
                     /locus_tag="PLD05_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOP76602.1"
                     /translation="MTQSREVSSPPGHPEVTSSLADRSSPATGGFSPRDASKEKTSQP
                     GKRHHFLRRLYDWVLHWADTPYGTPALFFLAFAESSFFPIPPDVLQIALSVSKPKRSF
                     FYAAISGTGSVLGGVLGYIIGFALWAAVGGFFMRYVPGLTAENIDYVGRLYQQNAFWA
                     IVCAGFTPIPYKVFTIAAGIFHEYVSLTILILGSVVGRFGRFFLVAACLYFFGPGVKA
                     LLEKYFDWAALGLAALAILGFVAIKWLL"
     gene            complement(16209..17255)
                     /locus_tag="PLD05_03890"
     CDS             complement(16209..17255)
                     /locus_tag="PLD05_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOP76603.1"
                     /translation="MASQMKRTRNSLAGVNLPDLEAALRRLIAAIPAGHVVSCGTLAE
                     VLGDFRAARWIGSWLGDHQHDPGCCCHRVVRAGGRWGPSPLSQEDREKLLLKEGVLLT
                     SSGADPRCLMDAAQLRALCPEVATKSPLLELQALQVRLCREIRLCPLEREPQSVGGVD
                     VSYRGDMGVAAYCRIAWPSGEKLWDTHIALPTRFPYIVGYLAFRELPLLQAVLDKAEQ
                     EGQLAEVIMVDGSGLLHPRGMGIASHLGLLLNRPTIGVTKTLLCGKCETEDLSPGQSA
                     PIVYRDQVLGAVLRSRTGHAQPVFVCPGHRIDVASAVKIVSPLFGAHRLPEPIYWADR
                     ISRSLARRFIPQNR"
     gene            complement(17287..18567)
                     /locus_tag="PLD05_03895"
     CDS             complement(17287..18567)
                     /locus_tag="PLD05_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOP76604.1"
                     /translation="MAKLDAHDACSAPIRQAGSQGRCHSSQRIGLTFSLYARNAVFFV
                     ILMLMIDTWLVSGIVYPIRVAGPSMMPSLDGPRFEIACPQCGYPLCWCAATQSFANCL
                     ERARCPNCGTRLARLMTSGNQQPQPNVRAGNPVVILKGLWRAWKRWDTVAFYNAGEHG
                     GLTVKRIVGLPGERTEIRDGVLWADGRLQPRPWEVQRSMCVPVNLPRNREDSVIHLDA
                     ASSLSQWEVEATHNTVTTYCHFHPALDQYEKPTRNLILRFSLERFSPGTRLHIRSRFG
                     SGHVEATLTLPSEFSVVCFDESPDGTSTKREQISGKLPPLIGEGEFAVSLVDQHFLLI
                     VNDRVITQMFYAARPLAKPIQRPFEVIVERGSVAIHNLGLWKVSCYDDEHRWADPLPG
                     YYVLGDDPHVSLDSRHFGIVAPDRVIGRVIKWPF"
     gene            complement(18643..19425)
                     /locus_tag="PLD05_03900"
     CDS             complement(18643..19425)
                     /locus_tag="PLD05_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76605.1"
                     /translation="MLRIRLAADLGPIDLPFQQALDHIREINLEAVELDLLKLMPVED
                     FTRTAVREIRKNLADRNLRLAAVRFRTLRGFDDPLGLEQRIAQAKKSMELTYQLGGNL
                     LLIRLGCLPKDRSSTDWQVLEEVVKEFCRFGERAGAIVVAEAERSPPEELAALLNGVP
                     EGGFGVDINPGELAVHGFDPVSAIETLGSRVVTFHATDATMRPGGHGYELAPLGSGGI
                     DYPPILSALEAKGFSGFLILRPCGERDPLTEVRRAKEFIYSL"
     gene            19625..22258
                     /locus_tag="PLD05_03905"
     CDS             19625..22258
                     /locus_tag="PLD05_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76606.1"
                     /translation="MMDYLSIAAFMLIVGFGGDPPKGSPLVPPAAQSTDFDTAYRCDY
                     SESFDQNYDNWPDYWIRHTGPRYPLYVKAEIVGEPSPEAARCLRVFLDGGSFAATSPK
                     FPVSSLFSYQVNVWVKTRGIVNDRVWVSYVVLDAHDKPLATFRSQPLGETPSWTLLQL
                     GPFEVDVPAASKAVVELHVEADVNRDVKAEVWFGNVHVHRMPSLRVVLDQPLGLYRLP
                     TRPVVNCHLSGVRSADTPLRITILNAEGKTVHDVELHPKYEKVETAVASAESAIHSQQ
                     VFSSQVQWQPDPLTPGFYWLVARIPWKDDAFRETRIPFVLMEAVPGSTSAFFGWSLPQ
                     GLEPLGARRLAELITESGVGWIKFPVWYDEQTSPAVLQDVPVLLDRLSNHGVKIIGVL
                     IPPQSVREALGPIAPSHTAGVFLRDQKVWEPSLTLSFVKMASLVPFWQIGGDNEHISA
                     NDPSLQKSFVQLKNALGQVVADIRLGVPWRWLEPPPKANIGFISLSEEPPLTAAELEN
                     YLARQSIASEKVFLLVELKPLSRQDYSIGLRVQDLVLRMASVRRASNVIGFHPNPFDP
                     DCGLFYPNGAPAELYLPWRTTIHAIGEGECLGSVALPAGSSNLIFARDDEGVMIVWNN
                     RTVRENVYLGEDIRVTDVWGTTLPYQGGPQGQSFLVDEWPRFVHGVSIPIARWRQEAN
                     IAVTRLPSIYGVPHRNKIRWQNTFDRAVSGEVTIVTKPGWRVTPQRFPFHLTPGEKVE
                     RDFVITLPFDAEAGPQPVRLDFKVGTDQFTEFSSYHFIEVGMGDVYVEITTRLDESGD
                     LIVQQVLVNDTPETVSFRCELFVPDRRRQFCLIQGQAPGRHVQTYRFPRGEELIGKTL
                     WLRAEEIGGSRILNYRVIATR"
     gene            22415..23056
                     /locus_tag="PLD05_03910"
     CDS             22415..23056
                     /locus_tag="PLD05_03910"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP76607.1"
                     /translation="MWRHLGFIVLAAAISCCLVSSVFVPTVAAEEEGFQPLFDSKTLN
                     GWEGDPRFWSVKDGVIVGSTDENKTTHNTFLCTTKTYKNFILKLEFRLRNHNSGVQIR
                     SKRLDDFIVAGYQADIADKRYMGILYEERGRGILADVNPEEVAKHVKPNDWNEYVITC
                     DGPKIRIQLNGFTTVDYEEKSPEGAKEGIIALQLHVGPPMQIEFRNIRIKELP"
     gene            23105..23677
                     /locus_tag="PLD05_03915"
     CDS             23105..23677
                     /locus_tag="PLD05_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76608.1"
                     /translation="MSDDVTFDPYRKWLGIPPEEQPPNYYRLLGIAPFESDPDVIVNA
                     ADRQMGHVRRFQAGRYAQVCQKILNELAAARVCLLDPVRKRAYDQKLFFEFRAAGREL
                     PFPVATPLAQREIPPPWGSEGVPATSGGSFSAVGEKGGAIAGPSVLSLYRRRRKSIWR
                     DPTTAILLTVLFLMVVVVAILVIFRQHFLG"
     gene            23741..25312
                     /locus_tag="PLD05_03920"
     CDS             23741..25312
                     /locus_tag="PLD05_03920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76609.1"
                     /translation="MAQSPLRDFSGREIGEFKILRRLGVGAMADVYLAEQPSLRRQVA
                     LKVLRLDLAQDETYLERFRREAQAAAKLIHANIVQIYEVGSENNIHYIAMEYVPGLNL
                     RQWLQKNGPADLRTALIIMRQVVAALVKAAEEGIVHRDIKPENILVTHNLEVKVADFG
                     LARLRPAEDGLELTRVGMTMGTPLYMSPEQIQGKPLDIRSDIYSLGVTCYQLLTGEPP
                     FKGENAMVVGVMHLTKPPEPLESKRPDLPPSFCRLIHRMLAKDPADRFSSPKVLLAEL
                     RKIYAEHVGEAWPENLPDLESSELSATMTAPAIKKLEEAMRLSQRRTTRRWILAVVAL
                     WVVGLAVGTLIGYWRINEPPLLAGTPTQPTLTVPKYDSIFAQWLHASQLGTEEAWRAI
                     IEYFPEKTYWTSRAKMRLAQIYLREDRLDEALRMFQELAASDDSEPELRAFGLAGMTG
                     VYVLQKRDQEASRALNELWPIRQYLRDPLMQRLVDYSIKSIRTRLGPMTAGDWDTWLN
                     EIKEEASINGPQDRP"
     gene            complement(25424..25711)
                     /locus_tag="PLD05_03925"
     CDS             complement(25424..25711)
                     /locus_tag="PLD05_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76610.1"
                     /translation="MSNSEPTKDKPQSRKIAGLVGIGLDGDDGQTRVTRGKNFVLWGG
                     SQETHAVMQETAIKVNERLDQKGKRMEDVSVNELRDIIHDVADSMGLKRRD"
     gene            complement(25730..26419)
                     /locus_tag="PLD05_03930"
     CDS             complement(25730..26419)
                     /locus_tag="PLD05_03930"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP76611.1"
                     /translation="MDVRLVVANGRYSGKSIRVTGSKFFIGRGEDCNLRPASDLVSRH
                     HCVLILDGDYLAVRDLGSKNGTFVNGQRVRTETPLKDGDHLLVGVLEFIVKTGAEAPK
                     AKPKVETVQEAVARTASASSTEFDVDQWLSEDEVTAESPADADTRTIQVKPAENAGGG
                     PPAPHATPEKPVDEETRKALASLFSGEEGKEEPAPGGKKQGDSGKFKQPQAADTRQAA
                     ASVLKSLFRRT"
     gene            complement(26896..28197)
                     /locus_tag="PLD05_03935"
     CDS             complement(26896..28197)
                     /locus_tag="PLD05_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76612.1"
                     /translation="MWTWFEQYRMDPTTWLYVASLMTIGIYFRFSRLFSIRNLDLIGI
                     IFFAPGLLLITHGLERAGYSWLFAVSALYLIRLLVDPAFARRPLMEPNLSSEGMTFMA
                     CALMFFLVANILTDRLVESQSRPETPVTESPIWRVPAHRGPGYLPFYRAITFTPQVPA
                     SRTASEGNLENLENAETSTLIVGAAIKIVAIAAQCFIVFGLILFGLRHFDNVKTGLAA
                     ASLYLLLPYTSQLTARLDHIVPGALIVWALVMYRRPMISGMLLGAATIVFYPFFLLPL
                     WIGFYQRRGLIRFLGGYLISVGTLVILLFFLSPTWHDFGNDLAAMFGKHLLRTEGADG
                     FWEYHWPYFRIPIFAGFAVLCVSLGLWPSEKNLANLLSGTAAVLLAAQFCLIHQGGLY
                     MAWYAPALILTVFRPTLEDRTAVRAVPRYWFGALPKLPGQL"
     gene            complement(28321..29793)
                     /locus_tag="PLD05_03940"
     CDS             complement(28321..29793)
                     /locus_tag="PLD05_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP76613.1"
                     /translation="MKRREFHQAAIGLGTAGLASFLGSTSRGEEAPPQEGDWYMEPPK
                     KLPVRRFDVVIAGGGTAGVVAALAAAAQGVKTALIEAKGYTGGTVTEGGTALHSFYNL
                     WKAFPGVEKRQVIRGFPQKIIDRLLSLGACSGHAEMEKGFNYDSVCTAIDTEMYKLVT
                     MEMLAEAAVEMFLNTMVVGAIRDGNRLLGAVTESRAGREAFFAHSFVDCTAYGDLAAF
                     AGADFTEPNDYAVANSIGLANVNVEKYYEFLESHDALTELARGLRSGQDGQIVRLEGR
                     LVNLPEDFRREATEIGMSTVTTTVHDNYFMFIKLNLKLPVSPTNRDAVTWAELELRRR
                     QARAVELFRKYVPGCEKAFIARTAPRLNIRRARCIRCDYDVTREDVLEGRHFPDEVFV
                     YGFHDMAPRYQIKDGGTYGMPYRALCVSGLDNLWCAGMLITSDYVAHMSTRNTVCSMA
                     QGQAAGTAAALCAQQKLTMRDLPYKLLRQTLEKDDVVFES"
     gene            complement(29872..30585)
                     /locus_tag="PLD05_03945"
     CDS             complement(29872..30585)
                     /locus_tag="PLD05_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOP76614.1"
                     /translation="MTQPCERRAFLHQVGLGSAMIGAGLGGSLLAEEAESQRQSKTVK
                     IVAVSCSPRKGKTTAQGLKIVLDAAKAVAPEKIETVLIELADLRIPVEPALGIPLPPG
                     ERDDFPAVAEQIADPRLKALIVGSPVHYANMSTLCKAFIDRLGQVFRKEFKLSGKLAG
                     ALAVGAARNGGQEYVLDSIHRALMCHEMAIVGDGRPTGHFGATLWNNGGNDDISTDEF
                     GVKTAQNLGRHIAELALKL"
     gene            31339..>31544
                     /locus_tag="PLD05_03950"
     CDS             31339..>31544
                     /locus_tag="PLD05_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76615.1"
                     /translation="MGSLVCFCTLVALLLATGCRTMDFYDRGLEQPAPTQELEPPREL
                     SKVSLPSYRLEPPDVVQIEVPKMVP"
BASE COUNT         7229 a         8799 c         8620 g         6796 t
ORIGIN      
        1 gaactcataa cgaacctccc tagtcagacg aaaaatgtgg aaacgaaaca tcaccgtaaa
       61 cttgccgact cacttcaacg gcaggttgac tattcaggca agggacaagc cctcgccaaa
      121 atcttgcacg ggttcacttg tgtttggcct gtttttgcgg ctcaacctgc agcgacacac
      181 aaaactgggc ttcattttga ttgatacaac ggctcataac aagtccccgc aaggagtctc
      241 cgtgtttgat cgtaagctta ggggatgtca acatcggcaa aatcggctga aaacaactaa
      301 gagcttatcc caaaaagtcc ccctctccct ccgggagagg gttggggtga gggcaagcga
      361 acgtcgggct cgattgagaa aaccggcagc gattcaagtc accctcaccc cggacacctc
      421 tcccgccgaa cgagagaggg gagattatgg gataggccct aaggcgatct gcgcccagaa
      481 gatgctgagg tagtggcaaa cacaatgccc gacgattaaa ctgggcaggt aattaatgtg
      541 taaactgact tgcgagtcac cgaatgtgag gagacatcac tgtgaagacg attggtgttg
      601 attcggcgaa aggccatcga gtgctcgctg ctttggccgc gagttgcttc gtatggggtc
      661 tcttggcaga tgtgacgcgt gctcagggaa caaatggggc cgaggggcct tttacagtgg
      721 agaagacaga gcatggtgtg agcgtcaaat acgacggcaa gctttgggca aactacgtgg
      781 ccgacggaga atttaaacct tatgtctggc ctatcattgg tcccacggac caaccggtca
      841 cgcgggaatg gcccatggga gaaggcctca atgaacgtca cgaccatccc catcaaaaat
      901 ccatgtggtt tacccacggc gatgtgaaca agaccgactt ttgggcggaa gggcctaacc
      961 gtggtcggat cgtccatcgc gagttcgtcc aggtctctgg gggaaagaac gctgtcatcg
     1021 tcacccgaaa tgattggctg ctccccgacg ggacgaaaac cctcgaggat acccgcaagc
     1081 tcatcttctc tcgttccggc gaccaccgca tcatcgattt cgtcatccgt ttgacggcga
     1141 cggccgacag tgtcaccttc ggcgatacca aagaggggac ctttggcctg cgggtggccg
     1201 agagcatggc ggtggatagc aaaaaaggtg gccaaattat gaatagtgaa ggccagactg
     1261 acgcgcaggc ttgggggaaa aaggcaaaat gggttgacta ttccgggcct gttggagatc
     1321 agattgtggg aattgccgtg ttaaatcatc ccaccagttt ccgattccca acgcactggc
     1381 atgttaggac ctatggtttg tttgcggcaa atccatttgg ttggcacgat ttcgtgggcc
     1441 gctccgatgt aagtggcgcc tacaccctcg gcaaaggcca gtcgattacg ttcgcctacc
     1501 ggattattct ccacaaaggc cgggctgaag atgcccgaat cgcccaggcc ttcgaggagt
     1561 acgccgccca accggtgggt gatctgcctt aaaagaccct tttcggcgcg tgagtcacca
     1621 gtggcccgcg acggattcgt ggggcgggga gtatgcgctt gccaagaacc tgccgtatct
     1681 tgcccaattt ctttagcctt gccagtcgtt tcaagttgta gaactgttac gattgattga
     1741 aggccggtcg ggacgggcgt cgaagtacta gatcagggat gttgcgcccc acggaggggc
     1801 tgcgcagacg tttcgcctga aaatagagat gagaaaaggc ccagcctttc cggcttggga
     1861 aagagacaat gagccaaatt gtggcaaagt cccccaacgg attaccgggc gtacccggcg
     1921 ggttgctcaa taattttgcc cagtgggtgg ccgatatcgg tgcccgcacg atggagtgga
     1981 ctatcggcct gggtagcatc agcctgtttt ttgggcggac acttcgctgg ctggtgacaa
     2041 aacgtccgcg ccgagagacg atcctttgga acttctatca gattggggtc ctcagcctgc
     2101 cggtggtggc cctaacaggg acgttcgtag gtatggtcct ggccgttcaa agttacgccc
     2161 aattcaaaat gctaaacttg gagacgcggc ttggggccgt tatcaacatg tcgctggttc
     2221 gtgagcttgg ccctgtcctt gcggccacga tgttggcggg ccgggtagga agtgctatgg
     2281 ccgcagagtt gggaacgatg cgggtgacgg aacagattga tgcccttagt gtgatgggnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat tgtgggctgc catcatgggt
     2461 ttcattgtga tcctggtgcg gagggtgttg ggcgggcggc gacctacgca ttcgttcatt
     2521 cgtttgtgat gatcctcctt ttggatttga ttttgggaat tggcctggat gccctttaca
     2581 acgtactgtt tccgcaaggt ccgcgtctcg tgtaacttgg cgagattgtc aaacgtcctc
     2641 aagcttcgtg taccgggaga cctttcggag gaacgaaatg cggtattggc aggggcgtta
     2701 tggcttaacc cccattgatt gtgtttcatg atcgctcagg caacgacgga aggaacgatt
     2761 agtagcccat catctaagga cccggtgttg ctcgaggtcg agcaactgac cgttgccttc
     2821 ggacggcagg tggtcctgcg cgggataaat gtacaaatcc gtcgcgggga aaccgtggcc
     2881 atcctgggag aaagcggctg cggaaagacg gtttttctca agacactggt ggcactgcag
     2941 cggccgacga aggggcgtgt cctatttgat gggcgagacc tgtttcaatt acggccacat
     3001 gagttggccg aacagaggat tcgcatgggg tttgtgtttc aacaagcggc cttgtttgac
     3061 agcatgacga ttggtgagaa catagccttt ccgttgcgtc agcacacccg attgacagag
     3121 gacgagattt acgaggtggt caaggcccgc ttgctcgaag tgggactccc cgaaaacgtg
     3181 ctgcggaaga agccgggaga aatttccggc ggcatgcgga aacgggtggg gttggctcgg
     3241 gcactcgcgc tggagccgga gcttatgcta tacgacgagc caactactgg cctcgaccct
     3301 atcatgagcg atgtgatcaa tgaactcatt cttcggacgc gacgtcagca ccccgttacc
     3361 agcgtgatcg taacccacga catgaggacg gccacgaagg ttgccgatcg aatcatcatg
     3421 ctttatcctc tgacgcgact taaaccggga gaatctcaaa tactctacga tggtccggcg
     3481 agcgaaattg agtcggcggc agatgaccgt gtcattcagt ttgtgcgggg cgaagcgagg
     3541 gaacggctga tggaaatggc ccgcgaggaa tcggaaaacg cggttgaaat cggaggggcc
     3601 gcccatggat gagcgtttga tgcaatttcg catcggcgtg atggtccttg ccagcattct
     3661 gattgcggcc atcctcattg tcacgttcgg ggagtggcgc tggccatttc ccgcggaata
     3721 cacggtttat attcgcttcc cagaggcccc tggtgtggcc gagcgatccc cgttgcgaaa
     3781 aagcggcata gtaatcggct acgtgagaag cgtcgagcta acaccggaag gaaatgtcct
     3841 ggtgaccgcc cgcgtggaac gaaaatacac gttgcggcac aacgaggtgt gtcgtctgaa
     3901 tcgagccctc ctgggggatt ccattctgga gttttccctc tcgcccgatc caaacgctcc
     3961 taaagacatc gtcaaggacg gcgatgtgat aaagggggtt gttcccccgg accccatcat
     4021 cgtgctgacc aatctccagg agcgcctggt gacggcaatc aattccgtga acgagacatc
     4081 gcaagcccta ggcgataccg ttcgatcggt caaccgaatc ctcacggaga acgaacagca
     4141 gcttaaagaa ctgctaagta atgccaatca gacggtgaca gcggctcaac agacggtgac
     4201 caacgccaat gaaatcatcg gcgatcccga attgaaaggt cgcttaaagg aggccctgag
     4261 ccgagttcca caaatggtcg atgagattcg cgccacctta gacgaaacga atcagaccat
     4321 tcgaacggca cgagcgaacc ttgagaatct gcgtggcgtt acagaaccgc ttgcccaaaa
     4381 tggtcccgcc attgtgcaac gccttgataa cagcctgacg aacctggagc gcgtcagtgc
     4441 ccaattggcc gagttcagcg aggccctcaa tcgggaggat acgactttgg gtaagcttgt
     4501 ccgggacccc acgctttatc aaaagcttga tcggactatg acgaacatcc aatcgttaac
     4561 gcaagacctt cgaccggtaa tcagcgacct gcgggtattc agcgataaaa ttgctcgtca
     4621 tcccgaaatg ctgggcgtcc gtggtgccct ggagcgtcaa gccggaacaa aaggccttcc
     4681 gaatcttccg tcctttacca atcaagggca ggtcgagaca tacccgatag aggttcccgc
     4741 gagcaaccag cggttctccg ttcttcctgg gacgttacgc tgaccgtgtg gactttcctg
     4801 gttggtgatt tctaggtggc cattccgcgt gcaaaggggc ccttcgtctg gttgtggcga
     4861 gctatcggcc ttggcaggga ggggaattgc ttcagtgaaa gaggagcagc cagatcagca
     4921 cgtggacgac gaaaaacacc gccgcaaaaa tgaagaagac ttggccctcg atcaaagagt
     4981 cgaggaataa tgctagggcc aaggcaccta tgtaagcaac gatccaccac atcaggcgaa
     5041 accgaagaaa aacgccaggg tcgggacggt gagcgctcat gtctagggca cgttcgacaa
     5101 gaatccgctc caagtcacgc ttcttaattt cacggcgaat ccactcggcg atggggcggg
     5161 cctcggcatc gtcctcgaga agttgactca aggccagagc ctgatcctgc ttccccttga
     5221 gcagcaggag cttagccatg agcgattggg cctggacatt gttcgggtct aaagccaggg
     5281 cctgacgtag ggagttttca gcctcctgat actgatgcat ccgtaattgt gccgctgcca
     5341 gaagactcca ccctaccgag ttttttggat cggcttgttg cgcacgttga gctgcctcta
     5401 aggctagccg ggggccgcga taatggacca tgaaacgggc atattcgcga aggatttgcg
     5461 gggaaccacc actttgggcg acggcctttt cccatgcttc ttgggcccaa cccaggcggt
     5521 gttcggccgc ggcggcttgg gcaaaaaggc ttaggaccgt gacatcgtcg ggcgataatt
     5581 cccgagcacg gcgcgcatcc tcaagggcat cgcgggaacg ctggaggtta agtaacgtgt
     5641 aggcccgcat actgtaggca acaggatcgg acggcgattc tgtcacccat tggtcggcga
     5701 ggctgagcgc gcgaagaaat tccttgcgat caagtgcgac aagcaccgtc cgcagtttct
     5761 cttcccgctc aaccgggtcc atagcccatt accttgagct aatagtcgga gatgaggcct
     5821 tggcctctga ctgaaggaac gttctcatct ccaatcggtg atagggtcgc ttcgtcacag
     5881 gaggaaaccc cgctgatagg tgcatcacaa ggtcttctgg ctcgaagacg acggactgaa
     5941 ttgacaagaa gcccaagttg acatcgtcca atttcttctg aactgtagcg acatcaattt
     6001 tgggaatgtt atatgcctca agcaattctc cgtagcgtcg cgaaatcaca aacactttaa
     6061 gttcaggtgt gcaaaagtga ttggtgcacg ctagcaatcc ccgctcggcc tcgcggactg
     6121 cgacggtttt tggcgtgatt tcggcaacga cggctcgctt agggtcgcaa agggcgatgt
     6181 tcaaaagggt tgtacgaggg cttttcctaa gatgggcgat ggcctcttca acgttggaac
     6241 aatgctccat gatttgccgc gtcatcatgg cgtagggtat tccatggggg gaacatatcg
     6301 gtgaaccgtc gttagtaaag tgtacttcat gcaccgccag acacagacca gcgtcattca
     6361 tgccggttaa acacccaaca aaaccgggaa agccaatcga gacgaaacga tgccccccgc
     6421 cgtcggactc atagaccttc acaatcgtgt accggtaaag ctggcgtggc gaaaagaaat
     6481 cgaggttgcg accaaagagg acctttccgg tggcacttcg ttcgggaagg acaatcaagg
     6541 aggagcaggc aatactgcgg taaatatcag gcaacacgtt gatggcaaaa agctcgtcgt
     6601 gattcaaacc agccgcctcg gccatagtca ccagttcccg ccgatagttc tccggcacat
     6661 atctccagaa attccgtcct cgcgcgataa gcccctccca gcccccggga tagttcaact
     6721 ggcggaggaa ctcttgaggg gcaatgacta aattcgctat ggcgtcggat gttagttttg
     6781 cctcctggat ggcaatttct tctggttttc cacgcaaaat caagaccgga acctgattca
     6841 cgatacgcaa ctcgccccgg tcatatgttc ccgcttcata aactggggcc gacgaagcct
     6901 cagactcttc aagtctttgc ggtagtaccg gaaaagcgtc ttccgaggca aagatgttat
     6961 tcagcacacg ctgcaagact gaccagggtg ctgccgaccg aggctggctt gcttgctctt
     7021 cggcaacaga caccgacgcg aaacgctggt caagcgctcc gattcccgaa tagaaggtaa
     7081 ccaggataat tccccagaca agacaaacct tccaaaacac agaaatcgtc actcgagctc
     7141 ccttgctgga catcattcgc tgacccttgc tcttagagta gtgtcacaca aaacccttac
     7201 ttcgagaccg aaaaccgtat gccgcgccct gcattgtcca tcctagacga gatttttcaa
     7261 tgcggcaaat aggccgtgca atatacaatg ccgccgggtg ggactctccg tctcaaccca
     7321 ttcgtgaaaa tcttacccaa aaccactcaa tactcaatgc gaatcaccag tgatctgcgg
     7381 aagtccggga ggagaagggg cgactcggtt ttgcccactt cccaggcgtt tttccacgga
     7441 tcataaaacc gcacggtttt gctctcttgg acgcgaaagt caggcggaag ttccagcttt
     7501 atcccgtgaa gggttgccgg cggttttccc tcggccagtt ccgtttgcca ggcgttctcc
     7561 cgatctcgac accagatgag tgtttttcca gtgcccttga gggcgtagac gtagagccgc
     7621 gggtgttcca gctccaccgg ttgaaagtgc tcggctgggg gatcaagatc gcggacgacc
     7681 tcggcaaatc ggccaaaatg ccaccacaga ttgtttttat caacataaac atcccagtgc
     7741 caaatgtggc ctggtcctgc ggcgcccgca aaaaacgggg caaaaagaac gtcgtgaagg
     7801 atgataccgt ctttgtcctt ttcataaagt ttgaagggac cgctgtggct tggctcgaca
     7861 gccccgcttt ctgcgagaag gatcggcttt tgaatgccaa acgagcggag gtctttgatc
     7921 gcctccgcag cgaagacggc gactgggccg tgacagattt tccaggctgc cccgaggtca
     7981 agatagcgat gcacttgcag gacatcgttg ccgggaagtt cacatagccg ccggtagcgt
     8041 tcacgtttgt tttcgtgatc gtaacttccc agactttgca tggccaggtt ttgcgggaaa
     8101 aggcggtgaa gctccggaag catctcggcc gtccacggtt cccatacgtt gaccctgacg
     8161 gcgtccatct cattccataa ttcccacccg aagatgatcg gatcactgcc atagcgagcg
     8221 gcgtaccagg ccagctttcg tttatattgg gcacgccccg cttcgcccag gaaaaagtcg
     8281 gccgtatctt tggccggccc accgttggcg attaggtgct gcggtttagc cgcccaagcc
     8341 tgcgtccctt caccgaggtg ccgaaagtgt tccgtacaca acttgaggcg aatcccatac
     8401 tctttggcca gggccagcag gccgtcgata cgtttggctc gctcttcatc gaataccccg
     8461 cttcgttcat gttccacatc aaaaaacgga ctgcttagcc aaatccgcac gaaattgcct
     8521 ccctcggcgg agagcttgga aaaccacact tccatcccgt ccagactatc ggtcggcggg
     8581 cgaatcatat tcagcccgat gggaatatag ggcttgccgt cggaaagctc aaagtaacgc
     8641 ggatcccgcg ggctaatgcg aacatagggc agcgactctt ctcctcgaga gacgacggca
     8701 gagaaccacc ctgtcgccat aacagccatg atgacaaaaa actgccttcg gaacttcatc
     8761 gtgggattct cctcgtccgc tagcttgcga tctgctccca ttttccacgg gtaaagtcgg
     8821 ggatttctac gggtttgccg ccttcattga gagactgctc agaaagcggc ataacgctgc
     8881 tccaagcggc ggcgtcatag acatcgaccg ggacaggccc acctttccgg acggcctccg
     8941 cgaactgctg aatgacaaag taatcgccgc cactgtggcc ggtcttttgg gctttttccg
     9001 catgttcttt ccacagagga ttatcaaact cttcgacata tggcccggca ggttcccagg
     9061 ccttggcctt ggagcgccct tctatccaga cggagtcgat ccgcgaatcg taaaccgcct
     9121 ttgttccctg gattgtgtaa taaacggtgg tagggtgcgg ccgagcactc atgacgtcgt
     9181 atctcacatc gatcagcact tccttcgctg ttttgataag cactgtggtc gagtcccccg
     9241 cccgaaagtc gatttgccga gcaggatgtc cctcgggaaa ccgctgctcc acataatacc
     9301 gcattgccgc ccggcccgtt tcagcagcca ccaagctgac tagacgatcg cctttgttaa
     9361 tgcccagcca cttggccacc ggaccaaggg agtgcgtgga ataccagttc cctctgtggt
     9421 cgcgcttgat ttctccccgc caagtgagcg tgccatcggc ctcgaaggcc aaggcccggc
     9481 agtcgtggac atatccgcat tccgcgaacg tgagctgtcc aaagaggccc gccgcaatca
     9541 tctgaccgat ggcgagcaca ggtcgccaga agcagcaatt ttcggccagc atataaatct
     9601 tgcccgtttc ttccacggca ttgacgaggt cccaacactc ctggaccgtc atagcggcgg
     9661 ccacttcgct gagcacgtgc tttcctgcgc ggagggcatc cactgccatc acggcgtgcc
     9721 attgcatggg cgtagcgatg agcaccgcct ccaagtcatc acgctctagc atacggcgat
     9781 aatcggtggg cgtgtcgcca taagtcgctg gtcgctgccc atctcgcatt tcggcgataa
     9841 gtttcccggc aatctccagt cgtgccggac gaatatcgca gactgccggg acctcggcac
     9901 cctgttccaa aagcaatcgc aaaaggtatg ttccccgacc acccacacca ataacgccta
     9961 ttcgcaaggg agcaccttgc ggcggggttt cttcagccga aaggacacct gctgcaactg
    10021 ccccggcggc aactgcggcc tgactcttga ggaacatgcg gcggctaaga gatgtgtcca
    10081 tttggggcaa cctccggagg cttaatcgtt gggccttgct cacttttgcc cctattatgc
    10141 ttattcctcc gggcaaagaa aagcttccaa gactcgacac agagccctgg tggtcgggcc
    10201 tggactgttg tcccgacttt ctcgaaagac tactcctgag gttcccaata atcgaactgt
    10261 tgcaattcat ccgggccagg gttggcgtaa tcggggtctt tcgcccgaat gaacacgtcc
    10321 ccatcgggaa gtgtctttat aaaaatgggc acaatcgtca cgccttgggg attgattgtt
    10381 tgtgccgtct cggagagatc cttgccacgg gcttcttcgc gcgaaagggg gggcacattt
    10441 gcattataaa aggcggtaca ccaccaatgg ccggccttgt cttgaaagag ggtgccatgt
    10501 cccaaaaacc gccccacgaa gcggcgaaca ctgtatggcc cctcgatacg atccgctgtg
    10561 gcatagtata ggttataact tcccttacgc atctgccggg tggaccagcc cgtaccgaag
    10621 agcacatact tgcgtccgaa cttcttgatg aagcatcctt catgtcccat cgtgcccaga
    10681 gttccgtccg gttcggtggg gccgatctta ataggctgtt gggcgaaatc actaaagtca
    10741 ggtttaagcg gagcaatttc cgtagccccc cagacaagcc accatgtgcc atcatcatct
    10801 cgaaagagcg acggatcgtg gcgatgacca atcttttcgc ccatggggtt cttccatggc
    10861 cccttgattt ctgggccttc cgtgagggaa agattggagg cacgaacagg cgacggactg
    10921 gtgtggacca acgcccaacg attgcccagc cagtgcaatt ctggcgccca gagacgccaa
    10981 gccgattccg gcacttgtcg gaagcgttcc ggctgcacct cggcccaaac cccgtctttc
    11041 aaactgaatg gctctccgca gtactcccag tggatcaggt ccggactctt ccaaagacgc
    11101 accttccatc ccacaatgct ttgggggccc agtcccgtgt tcagaatgtc gtttctttcg
    11161 cgggcatcgc caggcagggc cgtggtgccc gtcagatagt agtaaccatc cggcgcgaga
    11221 acaataaaag ggtcccgaat ccagccatcc ttaatgtgaa gggcccgatt gtgggcctgc
    11281 agaccggcaa cgatcgtttc cggtgtgtcg gccgcatgga cgaatattgg cagcagcgta
    11341 aagctgattc ccagcagaaa aatagcgcgc atcgtaacct ccccaagttt aaacgagatt
    11401 cttgcaaaca acaggctgac gccacgcccc tgaaggcctg cgcgtccatc atgatattgg
    11461 cactgtagaa gacctgccga catgtggcaa ggggcttgcg cacttgtaca ttcgatttgc
    11521 gcaaaagtcc attgctgaca ccagcgcgct tgaccacaat gaggggtgtt ggtcgtcgtg
    11581 agcacactat ttctatgggg aggagacaac gactatgaat ttctgggcaa tgccttcaat
    11641 gaaagcgaac aaactggctc tttcttggtt ggcatggatg ttgacaattg tcgctttggt
    11701 cgcgacctct tcggcaacgg aaacaagtca gcagcgccgc aacatattgc tgatctgcgt
    11761 cgatgacctc aagccggtcc ttgggtgtta tggtgatccc gtggctaaga cgccgcacat
    11821 ggatcggctg gcggctcgtg gcatgcgatt tgagctggct ttttgcaatc aggccgtgtg
    11881 tgccccatcc cgcaataact tgctgttagg ctcgcgttct acatccattg gaatctatgg
    11941 cttgagcgaa aactttcgcc gagcagtacc cgatgccgtt acgatgcctc agtatttcat
    12001 gaagtacggc tggcggaccg aagcggttgg taaggttttc cataccggtc acgggaacca
    12061 cgacgatgca gcatcttgga gcgtgcccag cgtcattgaa aaggtggtgg aatatgtgga
    12121 tcctcagaat tcggcaggag gacaactgac gcgagaggag gcctatttta ccaatcaaat
    12181 actggaccga atcggcagtt tgccccgcgg tgctgcctgg gagaaacttg atgtcgccga
    12241 tcaagcctat gccgatgggc gaatcgccga cgagggtatc aagcgattac gagcagccaa
    12301 agaacgccgc cagaaggagg gtacaccatt tttcctagcc ttgggcttcg ttaagccgca
    12361 tctgcctttt acagcaccaa agaaatactg ggatctctac agtccgactg aattcaagct
    12421 ggcctcttat caagagcccc ccaagggggc gccgcctgtg gcaaataaac gcggtgggga
    12481 aatcttgaac tacgatcccc ttactatgga aaacctgaag accgatgaga ttcagcgcac
    12541 gctgatccac ggctattatg cggccacaag ttttgcggac gcccaaattg gtcgggtgct
    12601 tgatgaactt gaccgactag gactggcaga atcaacgatc gttgtcctgt ggggtgataa
    12661 cggtttccat ttgggcgatc acggtatctg gaccaaacat accaattatg agcaggcggt
    12721 ccgaattccg ttgatcatcg ttgctccggg tgtgacaccg ccaggatcgg taacacggca
    12781 agtggctgaa aacgtcgata tctttccgac actggccgag ctggccggac taccgcgccc
    12841 caccggcccc cagccaattg acggcgtcag cctggtcccg gttcttcgca atcccgatac
    12901 tcgcgtggac gatcacgctt accactgttt tccgcgaggt gccgtgatgg gtcgtgccat
    12961 tcgtacagaa cgttatcgac tggtggagtg gaaaaagatc ggtgccccag cagaaacggc
    13021 agaatatgag ctctacgact accagacaga ccccgaagaa cgagaaaacc tcattgacaa
    13081 gctgcccgat gtcgcggccc aactgaagaa gattcttgcc cagtaccccg aagcggccat
    13141 ccctgtcacc cagcgacgcc aaggcaatcc tgtggtgaaa ccatgaggcc tacacctata
    13201 atcgacaata ctttgtggac agttttgatc gcttttttgg ggaggattac tcgatggcgt
    13261 gcaaagttcg gtcattgctg cttgttacca tcttgggaat tgttccattt gtctgcttgt
    13321 gcccgaccct gagggcttgg gccaacgagg gaaagcccta tttgcggccc aacattgtgt
    13381 atatcgtttc cgatgagttg ggttattacg agctctcgtg catgggacat ccccactttc
    13441 aaacgcccaa tatcgatcgt ctggccgctg aggggattcg gtttacacaa gcacttgccg
    13501 gaagctcact ctgcgcgcca acacgatgct gtttgatgac gggtaaacac tcaggacata
    13561 cttcgatccg caccaacggt ggtggaacac cactccgacc ggaggaaacc acaatcgcct
    13621 cggtgctcaa acaggttggt tatgccactg gtggatttgg caaatggggg tgtggcggac
    13681 gtggttccac aggggtgcca gaacttcacg ggttcgacat ctttttcggt tattatgatc
    13741 aagttcacgc ccactcctac tatccgcctt acctgattcg caacagtgag gaggtccccc
    13801 tgccaggcaa ccgcggcttg agcgagggca ccacatactc gcattatctc attgtggaag
    13861 aggccaagaa atttatccgt gagaacaagg atcgtccctt tttctgctat ttgcccgtga
    13921 caccaccgca tggtattcac gatattcctg aaaccgaccc cgcttgggcg atctaccgcg
    13981 acaaaccttg gccgctggat gccagaaaat acgccgccat ggtcaatatg gtggaccgcc
    14041 atgtggggga aatagtggat cttctcaagg agctgggact ccgtgagaaa accatcattt
    14101 tcgtatccgg tgacaacggt ggcgccgagt atttccgaga tgctgatcat cccagagggt
    14161 tccacgctcc caatgtgaac ccggttaccg gcgtccagtt ccgcggagga aaaggcaatc
    14221 tgtatgaggg cggactccgc gtccccatga ttgtctgctg gccaggccgg atcaaacccg
    14281 gtcgggttag cgaccttctt tggtacttcc ccgatgtcct gcccacgctt gccgagattg
    14341 ccggggcgac gcctccgccc gacattgacg gaatatctat cgttcccgag ctccttggag
    14401 aagaggtagt cggtcgcaaa caaagccagc acgattatct gtactgggaa ttgagtggac
    14461 aaatagccgt acggcgagga acctggaagg cgattcggcc aggggcggga aaaccgtggg
    14521 agctttatga tctgtcgaag gacatcagcg aaacgatgga cctgtcggct gaacatccga
    14581 aaatactcaa ggaattgata gccctggcgg aaaaggccca tcagccggcg gtggaaggtg
    14641 atttcctgca accggagttg aacgaaaaag accgccgcgc gaagttcggc gacgctgttc
    14701 cgcccgaaaa aatcaacgtc gcgcttgtcc aaaatatgcc gcaggctggg ctgattcctc
    14761 gcaagaatta tcggatcgta tcgtgcagca gcgaatcgat atccaacggt cgtttggccc
    14821 agcacgctat tgacggcaat ccccggactt ggtggcacac ccagttttcg cctgaggtca
    14881 aacgcccacc tcacgagttg gtcatcgacc tggggagtga gcacatcatc cgtggtgttc
    14941 gctatctcgc ccgacaggat gccacatgga atgggacgat caaggactgt gacctgacgg
    15001 tcagtgcaac accggagttc ccagacgaac caaccgtgaa attacaattc agcaagacga
    15061 gggaatgcca ggagcggtta ttcccagagg tccgaggccg ttacgtccgc ttgcggattt
    15121 tctcggaggt caacggaggg ccttgggcct ccatagccga gcttggcttt gtgggcgaat
    15181 agccatctgg tgatcggatt catagtaacc acttgatagc cacgaatcct aggatggcaa
    15241 gggcggcaag gcccaaggcg gcccaatcga aatatttttc cagcaaggcc ttaacgccag
    15301 gaccaaagaa gtagagacaa gcggcgacga ggaaaaaccg cccgaatcgg cccaccaccg
    15361 aacccaggat gagaatggtg agcgaaacgt actcgtggaa gataccggct gcaattgtaa
    15421 acaccttgta cgggatcggt gtgaagccgg cacacacgat cgcccaaaaa gcgttttgct
    15481 ggtaaagccg gcccacgtag tcgatgtttt cggcggtaag accgggaaca taccgcatga
    15541 agaaaccgcc taccgccgcc caaagggcga agccaatgat gtatcccaac accccgccca
    15601 agaccgagcc cgtcccactg atcgcagcgt agaaaaagga tcgcttgggc ttggagaccg
    15661 acaaggcaat ctgaaggacg tctggcggaa tcggaaagaa agagctttcc gcaaatgcca
    15721 agaaaaatag agcgggcgtt ccataggggg tatcggccca atggagaacc cagtcataga
    15781 ggcgacgaag aaaatggtgt cgcttgccag gttgcgacgt tttttctttt gatgcatccc
    15841 gcggggagaa accaccggtc gctggcgatg accgatctgc gagcgaagaa gtaacttcgg
    15901 gatgacctgg gggagaggaa acttcgcgtg actgtgtcat aagtttttca accaagactc
    15961 tgaggactaa cccactaccc gattatcctt cacagataaa caggcggcaa ggcaaaaggt
    16021 cgttgtttgg gggatttaag agcctatctt aaaaaagtcc ccctctcctt ccgggagagg
    16081 gttggggtga gggcaagcga acgtcggcat cgattgagaa aaccggcagc aattcgaggc
    16141 accctctccc ccgacccctc tcccgcggaa cgggtaagaa gagattatgg gatagactct
    16201 tatgtgtctt aacgattctg cggaataaac cgccgcgcca ggcttcgtga aatcctatca
    16261 gcccagtaga tgggctcggg aaggcggtgg gccccgaaca agggacttac gatcttcacg
    16321 gcactcgcca cgtcaatgcg atgaccggga cagacgaaca ccggctgggc gtggcccgtc
    16381 cgactccgga gcacggcccc caggacttga tccctgtaaa cgatcggggc actttggccg
    16441 ggcgataggt cttccgtttc acatttcccg cacagcaatg ttttcgtgac gccgatggtg
    16501 ggacgattga ggaggagacc cagatgactg gcgatgccca tgccccttgg gtgaagaagg
    16561 ccactgccat cgaccatgat gacctccgcc aattgcccct cttgctcggc cttatccaga
    16621 acggcctgga ggagtggcag ctcacgaaag gcgagatagc ccacgatata agggaatcgc
    16681 gttggcagtg cgatgtgcgt atcccagagt ttctcgccgc tcggccaagc gatgcgacaa
    16741 tacgccgcca cacccatgtc ccctcgatac gacacatcaa caccgcctac cgattgcggc
    16801 tcacgttcca gcgggcaaag tcgaatttcg cggcagagcc tgacttgcaa ggcctgaagc
    16861 tccaaaagcg gtgacttcgt tgcgacttct ggacataacg cacgtagttg cgcggcatcc
    16921 atcaaacacc ttggatccgc tcccgacgat gttaacagaa cgccttcctt caggaggagc
    16981 ttttcccgat cttcctggga aagtggggaa gggccccatc ggcctccggc tcgtaccacg
    17041 cgatggcagc agcaacctgg gtcatgctga tgatcgccca accaggagcc aatccaccgg
    17101 gcagcgcgga aatctcccaa aacctccgca agcgttccgc aacttacgac atgaccggcg
    17161 ggaatcgcgg cgatgagacg tcgcaaggcc gcctcaaggt caggcaaatt aacgccagcc
    17221 agcgagttcc ttgtccgttt catttggctt gccatgggcc aatgtccgca tatgggtaag
    17281 cgacctttag aacggccatt tgatcactcg gccaataacg cgatcggggg cgacaatccc
    17341 gaaatggcga ctatccagcg agacatgggg atcatcgccc aacacgtaat aaccgggcaa
    17401 aggatccgcc cagcggtgtt cgtcatcgta acagcttacc ttccaaaggc cgagattgtg
    17461 aattgcgacc gaaccgcgct ctactattac ttcaaaaggc cgttgaatgg gcttggcgag
    17521 gggacgggcc gcatagaaca tttgcgtaat cacacgatca ttaacgatca ggagaaaatg
    17581 ttggtcaacg agggagactg caaattcacc ttcaccgatc agcggtggca gcttccccga
    17641 gatttgttcg cgtttcgtgc tggtaccgtc agggctctca tcaaaacaca caaccgaaaa
    17701 ctcggatggc agagtcagtg tggcctcgac atggccggag ccgaagcgcg agcggatgtg
    17761 aagccgtgtt cccggtgaaa atctttccag ggaaaaacga aggataaggt tccgcgttgg
    17821 tttttcgtac tgatccaagg ccggatgaaa atggcaatat gtcgttaccg tgttgtgggt
    17881 tgcctccacc tcccattggg acaacgacga tgccgcatcg aggtgaatca cagaatcctc
    17941 cctattgcgg gggagattga ccggcacaca catgctccgc tgaacctccc aagggcgggg
    18001 ttgcaagcgg ccgtccgccc aaagaacgcc gtcgcgaatc tcagtccttt ccccgggaag
    18061 ccccacaatc cttttcacgg taagaccgcc gtgctcgcca gcgttataaa aagccacggt
    18121 gtcccagcgc ttccaagctc gccaaagccc tttcaagatc acgacgggat tgcccgcccg
    18181 aacgttcggc tgcggctgct ggtttccgct ggtcatcagt cgggctagcc gggtcccgca
    18241 gttgggacat cgggcgcgtt cgaggcaatt agcgaatgac tgggttgccg cacaccaaca
    18301 caggggatag ccgcattgcg gacacgctat ctcaaacctt gggccatcca gtgagggcat
    18361 catggaggga ccggcgacgc gaatgggata gacaatcccc gacaccagcc aggtgtcaat
    18421 catgagcatg agaatcacaa aaaacacagc attccgcgca tacagcgaga aggtaagtcc
    18481 aatccgctga gacgaatggc aacggccctg gcttccggcc tgtcggatcg gggcggaaca
    18541 agcgtcgtga gcatccaact ttgccatcgg cggcgccgtc acacgcttac ctcacgttct
    18601 tggcaaaaag aggcagaact ggagaaatgg gcggttatac tctcaaaggc tgtagataaa
    18661 ctccttggcc cgccggacct cagtgagggg atcccgctct ccgcatggcc tgagaatcaa
    18721 gaaccccgaa aaacctttag cctccaaggc actcaggata ggcggataat caatgccacc
    18781 actccccaaa ggtgctaact cgtagccatg tccccccggt cgcatcgtgg catctgtagc
    18841 gtggaatgtg accacccgtg agcccaaagt ctcgatcgca gaaaccgggt caaaaccatg
    18901 aactgccagc tctccgggat tgatatctac cccgaagccc ccctctggaa ccccgttaag
    18961 gagggcggcc aactcctccg gcggcgagcg ctcggcttct gccacaacga tcgctcccgc
    19021 tcgttcacca aaccggcaaa actccttgac gacttcctcc agcacttgcc aatcggtaga
    19081 agaccgatcc ttcggcagac atcccaaccg aatcaggagg agattccccc ccaattggta
    19141 cgtcagttcc atagacttct tggcctgcgc gatgcgctgt tccaacccca ggggatcatc
    19201 aaagccgcgc aacgttcgga atcgtactgc cgcgagtcga agattccggt cggctaagtt
    19261 ttttcggatt tcacggacgg ccgttctggt aaaatcttcc actggcataa gctttaatag
    19321 atcgagttcc accgcctcca aattgatctc gcggatgtgg tcaagagctt gctgaaatgg
    19381 taggtcaatg gggcccaagt ctgcggcaag tcgaattcgc agcaccgttt tgatcctcct
    19441 aaacgttttt caatgcttaa gccgaagcgc ttccaggaga actttagacc acccgcagca
    19501 aagtccgatg atacaaattt gcccaacgtg gcaacgcacc ccacgtaagg attcacacac
    19561 cacggtgtgg agagtgatca acgctcgaaa accgcggggc cgcgctcccg gacggacaca
    19621 caatatgatg gattacctgt cgattgcggc tttcatgctc attgtagggt ttggagggga
    19681 tccacccaag ggttctccct tggtgccccc agcggcgcaa tcgaccgatt ttgatacggc
    19741 ttaccgttgc gactattcag agagttttga tcaaaactac gacaactggc ccgattactg
    19801 gatacgtcat actgggccga gatatccgct gtacgtcaag gccgagatcg ttggagagcc
    19861 ttcgcccgag gcggcgcgtt gtctaagggt gtttctcgac gggggttcct ttgccgcaac
    19921 atcacccaaa tttcctgttt cttctctttt cagttaccaa gtcaatgtgt gggtgaagac
    19981 ccgggggatc gtgaacgacc gtgtttgggt gagttatgtc gtcttggatg cccatgataa
    20041 gccccttgcc acgttccggt cacaaccctt gggtgaaacg ccaagttgga cactccttca
    20101 attggggcct tttgaggtgg acgtcccggc ggcttccaaa gcggttgtcg aactacacgt
    20161 ggaggccgac gtcaatcgtg atgtgaaggc cgaggtgtgg ttcggcaacg ttcacgtcca
    20221 tcggatgccg tcgctccgcg tcgtcctcga tcagccgttg ggcctttatc gactgcctac
    20281 ccgcccggtg gtgaactgtc acctctccgg tgttcggtcg gccgatacac cgcttcgcat
    20341 cacgattctt aatgcggagg gaaagactgt ccacgatgtt gagctccatc ccaagtatga
    20401 aaaggtggag acggcggtgg catcggctga atccgcgatt cacagtcaac aggtgttctc
    20461 gtctcaggtc cagtggcaac cggacccatt aacgcccggt ttctactggc tggtggcccg
    20521 cattccctgg aaagacgatg cctttcgtga aacccgaatc ccatttgtgc tcatggaggc
    20581 tgtcccgggg agtacaagtg cgttctttgg gtggtcactg ccgcaaggat tagagccact
    20641 gggtgcaagg cggcttgcgg agttgatcac agaatctggc gtaggttgga tcaagtttcc
    20701 tgtgtggtat gacgagcaaa cctctcccgc cgtccttcaa gacgtccccg tgctactgga
    20761 tcggctaagc aatcatgggg tcaagatcat tggtgtgctt attcctcccc aaagcgttcg
    20821 tgaggcacta ggtcccatag ctcctagcca cacggccggg gtattcctcc gcgatcagaa
    20881 ggtgtgggaa ccttcgctga cgctgagctt cgtgaagatg gcctctttgg ttccgttttg
    20941 gcaaatcggg ggggacaacg agcatatttc ggccaatgat ccatcgctgc aaaagagctt
    21001 tgtccaactg aagaacgctt tagggcaggt cgtcgctgat atccgtttgg gcgtaccttg
    21061 gcggtggttg gagccacccc cgaaggccaa catcggcttc atctctttgt ctgaagaacc
    21121 accgttgacg gccgctgagt tggagaacta tttggctcgg cagtcgatcg cttcggagaa
    21181 ggtcttcctt cttgtcgagc tcaagccact gagtcgccaa gactactcga ttggacttcg
    21241 cgttcaagat ctggttttgc ggatggccag tgtgaggcgg gccagcaacg tcattggctt
    21301 tcaccctaat ccttttgacc ccgactgcgg attgttctat cccaatggtg cgcccgcgga
    21361 gttgtatctc ccttggcgga cgaccatcca cgccattggg gaaggcgagt gcttggggtc
    21421 ggtagcccta cccgctggat cttccaacct gatttttgcc cgagacgatg aaggtgtcat
    21481 gattgtctgg aacaatcgaa cggtccgaga gaatgtgtac ctgggtgagg atattcgcgt
    21541 gacagatgtc tgggggacga cattgcctta tcaaggtggt ccgcaggggc agtcattttt
    21601 ggttgacgag tggcctcggt ttgttcacgg cgtttccatc cccatagctc gttggaggca
    21661 agaggcaaat atcgcggtca cccgtttgcc cagcatctat ggtgtgcctc atcgcaataa
    21721 gattcgctgg cagaatacat ttgatcgggc ggtttcgggt gaggtgacga tcgtgaccaa
    21781 accggggtgg cgggtgacac ctcagcgatt tccttttcac ttgacgccgg gtgagaaagt
    21841 tgagcgcgac tttgtgatta cccttccgtt tgatgccgag gcgggccctc aacccgtgcg
    21901 gttggatttc aaggtaggaa cggaccaatt taccgagttc agtagctatc atttcattga
    21961 agttggtatg ggcgatgttt acgtggagat caccacgcgg ttggatgaat ccggtgacct
    22021 tattgtccaa caggtgcttg tcaacgacac gccggagacg gtgtccttcc gctgtgagtt
    22081 gtttgttccc gatcgacgtc gccagttctg tttgattcaa ggccaagcgc cgggtcgcca
    22141 tgtacagacg taccgctttc cacgcggaga agaactgatt ggaaagaccc tctggcttcg
    22201 cgcagaagaa atcggaggga gtcgtatcct taattatcgt gtgattgcga cccgctaatc
    22261 gctcgtcgga cgtagctatg cctgcccggg atagagacag acttgagtct ggtccaacgg
    22321 ggtggtattc tggctgtagg ttacggtctc tttaagcacg tgcagattgt gcgcgttcgc
    22381 tgtcctgagg tccagttcca gaggagcaca aacgatgtgg cgacaccttg gctttattgt
    22441 tttggcggcg gcaatctcgt gttgtctggt atcatctgtg tttgtgccaa ccgtggcggc
    22501 agaagaggaa ggttttcaac cgctctttga tagcaagaca cttaacggct gggaaggaga
    22561 cccgcgattt tggtcggtga aagacggcgt gattgtggga agcaccgatg agaacaaaac
    22621 cacccacaac acattcctct gcacaacgaa gacttacaaa aacttcattc tgaaactgga
    22681 atttcgcttg cgaaaccaca attcgggcgt ccagattcgc agcaaacggt tggacgattt
    22741 catcgtggct ggttatcagg ccgacattgc ggacaaacgc tacatgggga tcctctatga
    22801 ggaaagaggc cggggaatcc tcgccgatgt gaatccagaa gaagtggcga agcacgttaa
    22861 gcccaacgac tggaatgagt acgtgatcac gtgcgatggg ccaaagattc gcatccaact
    22921 caacggcttt acgacggtcg attacgaaga gaaaagccca gaaggggcga aagaaggcat
    22981 cattgcattg cagctccacg ttggtccgcc gatgcagatt gagtttcgaa acatccgaat
    23041 caaagagcta ccgtgaagct ggggtttccg gttcgatcac gtcatacgag ccgcatttga
    23101 ttcaatgagt gacgatgtca cattcgatcc gtaccgaaaa tggctaggga tcccgcctga
    23161 ggagcaaccg ccgaattact atcggctgtt aggaatcgcc ccgtttgaat cagacccgga
    23221 tgtcatcgtc aatgccgctg atcgccagat gggacacgtt cggcggttcc aggcaggtcg
    23281 gtatgcgcag gtatgccaaa agatcctcaa tgagttggcc gccgcaaggg tttgcctcct
    23341 agatccggtc cgcaaacggg catatgacca gaagctcttt ttcgagtttc gggcggcggg
    23401 acgggagttg cccttccctg tggccacacc gcttgcgcag cgagaaatcc ccccgccatg
    23461 ggggtcagag ggggtgcctg caactagtgg cgggtctttt tccgcggtag gagaaaaggg
    23521 gggagcgatc gctggcccaa gtgtcctgtc gctctatcgc cgacgccgca agagtatttg
    23581 gcgggacccg acgacggcaa ttcttttgac cgtgctgttt ttgatggttg tggtcgtggc
    23641 aatccttgtt attttccggc aacattttct gggatagctt accgtggtgt gagcgtcccg
    23701 aaccattgtg gcgaaccatc ccctggtagg agatgacagc atggctcaat cacctctgcg
    23761 agacttttct ggacgcgaaa tcggcgagtt taaaatcctt cgccgtttgg gtgttggggc
    23821 catggcggac gtctatctgg ccgagcagcc ctcccttcgc cgccaggtgg cgttgaaggt
    23881 cctccgtctt gacttggccc aggacgaaac gtatttggag cgattccggc gtgaagccca
    23941 ggctgctgcc aaattgatcc acgccaacat cgttcagatc tacgaagttg gctcggagaa
    24001 caacatccat tacattgcca tggagtatgt gccggggctg aacctccgcc agtggctgca
    24061 gaaaaacggt cccgccgatt tgagaaccgc cctcatcatt atgcgtcagg tcgtggccgc
    24121 cctggtgaaa gccgccgaag aaggcatcgt tcatcgtgac atcaagccgg aaaacatcct
    24181 ggtcacgcat aatttagagg tcaaagtggc ggatttcggc ctggctcggt tgcgaccggc
    24241 tgaagatgga ttggaattga cccgcgtcgg aatgacgatg ggaacgccac tttatatgag
    24301 cccagaacaa atccagggaa agcccctgga tatccgcagc gacatttact cgttgggggt
    24361 gacgtgctat cagcttctca ccggtgaacc acccttcaaa ggggaaaacg ccatggtggt
    24421 cggcgtgatg cacttgacca agccccccga accattagaa agcaaacgtc cggacctgcc
    24481 gccaagtttc tgccgcctga tccaccggat gctcgccaaa gatcccgcgg atcgtttttc
    24541 ctcccccaag gtccttttag cggagttgag gaaaatctat gccgagcatg tcggcgaagc
    24601 ctggccagaa aacctaccgg acctcgagtc gagcgaattg tccgccacga tgacggcccc
    24661 ggccatcaag aaactcgagg aggcgatgcg tctttcccag cgccgaacga cccgccgttg
    24721 gatacttgcc gtggtcgcgc tttgggtggt ggggctggca gtcggcacgc tcattggtta
    24781 ctggcggatc aacgaaccac ccctgctcgc cggcacacct actcagccaa ctctcactgt
    24841 cccaaaatac gatagcatct ttgcccaatg gttgcatgct agtcagttgg gcacggaaga
    24901 ggcatggcgt gccattattg agtactttcc ggaaaaaacc tactggacat cgcgggccaa
    24961 gatgcgattg gctcaaatat acctccggga agaccggctg gatgaggccc tccgcatgtt
    25021 ccaggaactt gccgcgagcg atgattccga gccagaactg cgggcgtttg gcctggccgg
    25081 aatgaccggc gtttatgtgc tgcaaaagag agatcaggaa gcatcacggg cactcaacga
    25141 gctttggccg atccgccagt atctccgcga tcccttaatg cagcgcttgg tcgattattc
    25201 catcaaatcc atccgcacaa gactcggtcc tatgaccgcc ggcgactggg acacctggct
    25261 gaatgagatc aaggaagagg ccagcatcaa cggtccacaa gaccggccgt gagtggccag
    25321 ttgagaaacc ttgccccttg ttcctggttg tcgcctatac cccaagaaac aaagcaagcc
    25381 gcaagtaggc gtaatgccac tgcgggtatg agttgagggc agctcaatcc cggcgtttga
    25441 gtcccatgga atcggctaca tcgtggataa tgtctcgcaa ctcgtttacg gacacatcct
    25501 ccattctctt acccttttga tcgaggcgtt cgttgacctt aatggctgtc tcctgcatca
    25561 cagcatgtgt ctcttgactg ccaccccaaa ggacaaaatt ttttcctcgt gtcacgcggg
    25621 tctggccatc atccccatca aggccgatcc ccacgaggcc cgcaattttt cgcgattgcg
    25681 gtttatcctt ggtcggttcc gaattgctca cgtgcttcct ccgcgtgatt tacgtgcgtc
    25741 taaagaggga ctttaaaacg ctggccgccg cttgccgtgt atcagccgcc tggggttgct
    25801 tgaacttgcc cgagtctcct tgtttcttcc caccgggcgc cggctcctcc ttaccctctt
    25861 caccgctgaa gaggctggca agagctttgc gagtttcttc gtccacgggt ttttccggcg
    25921 tggcatgggg agctggcggc ccaccacctg cattctccgc gggcttgacc tgaatcgtcc
    25981 gcgtgtcagc gtcggcgggt gattcggcgg ttacttcgtc ctcgctcaac cattgatcga
    26041 catcgaattc ggtcgagctg gccgaggctg tccgggcaac cgcttcctgg acagtctcca
    26101 ccttcggctt ggccttgggg gcctcggcgc ccgttttcac aatgaactca aggacaccga
    26161 ccaagagatg atcgccatcc ttgaggggcg tttcggtcct gacccgctgg ccgttaacaa
    26221 aagttccgtt ttttgacccc aggtcccgca ccgcgagata atcaccgtcc aaaatcaaga
    26281 cacaatggtg gcgactgacc agatcgcttg ctggacgaag gttacagtcc tcgccgcggc
    26341 caatgaaaaa cttcgaaccc gtcacccgaa ttgacttgcc cgaatagcgg ccgttcgcaa
    26401 ctacaagtct gacatccatc gccatacgcc tattctaaaa aaggtcacgc aacgtaaacg
    26461 ttgccaaaaa ccgacgacct cgaacgttat tcttgtgttc cggaatccaa gcacggaaca
    26521 cacccaggcc ccccaactcg cgatctcccc gttgggatcg atcatgtgca ccgagtgtct
    26581 ggcccgcccc tccgttccaa ctctcgtcgg cgcttccatg gcacggcgac ctacccttag
    26641 ctacctctta ccactgccga agctccgcgt cggcgatgag tgcacgacac actgcctttt
    26701 caccctctat catcgtagca aggacggaca cacaagtcaa agccgcccat cggttttttg
    26761 ggccgattta ctgaaaatgc gcgcatgatc ctaactgtgt tccggaaaaa tatttacggc
    26821 tcaactgtct ctctcatgtt cctttgccgc gttccgcaag gtggaccatc ccggacgccg
    26881 gtgctgtgct gatcctcaga gctggcccgg aagtttcggc aatgcgccaa accaatagcg
    26941 tggtacggcg cgaaccgccg ttcggtcctc aagcgtgggc cgaaaaaccg ttaaaataag
    27001 tgcgggggcg taccaggcca tatacaaacc gccttggtgg atcaaacaga actgagccgc
    27061 cagtaacacg gcggccgttc cactgagcag gtttgccagg ttcttctccg acggccaaag
    27121 gcccaaactc acacaaagaa ccgcgaatcc cgcgaagata ggaatccgga agtacggcca
    27181 atgatattcc caaaagccat cggctccctc ggttcgcaaa agatgttttc caaacatagc
    27241 cgccagatca ttgccaaagt catgccacgt cggtgagagg aagaacagaa gtattaccag
    27301 ggtaccaaca ctgataaggt agcccccgag gaatcgtatc aggccgcgtc gttgataaaa
    27361 accgatccaa agcggcaaaa gaaagaaggg atagaagaca atcgtcgcgg ctcccagcag
    27421 catcccggaa atcatagggc gacggtacat caccaaggcc cagacgataa gtgcaccggg
    27481 gacaatatga tccaatctcg ctgttagctg gctggtgtaa ggcaatagca aatataggga
    27541 ggccgctgcc aagcccgttt tcacgttatc aaaatgccgc aaaccaaaga gtatcaagcc
    27601 gaacacaatg aagcactggg cagcaatcgc aacaatctta atggccgctc cgacaattaa
    27661 cgttgatgtc tcagcattct caagattctc gaggttaccc tcggatgcag tgcgtgaagc
    27721 cgggacctga ggcgtaaatg tgatggcccg ataaaacggc agataaccag gaccacggtg
    27781 agcaggaacg cgccagatag ggctttcggt caccggcgtc tcgggacgag attgtgactc
    27841 caccagccga tcggtcaaga tgttggcaac gagaaagaac ataagggcgc acgccataaa
    27901 cgtcatgcct tcggacgata agttcggttc catcaaggga cgacgcgcga aggcgggatc
    27961 gaccaaaaga cggatgagat acagggcact cacggcaaat agccaagaat atccggcccg
    28021 ctccaaccca tgcgtgatga gcaataaacc tggcgcgaag aagataatgc cgatcagatc
    28081 aagattgcga atggaaaaaa gacgcgagaa ccgaaaatag atcccaattg tcattagcga
    28141 cgcgacataa agccacgtcg tgggatccat acggtattgt tcaaaccaag tccacatagt
    28201 ccgagattat ctagtccagg gacattgctg actacctcca gccggtccgg cttgcgaaaa
    28261 acctgccacg gtttgcccgc ttgaccgctt tgccctaacc cacccgatga cgggacgtca
    28321 ttagctctcg aacacaacat cgtctttttc gagtgtctgc cgaagcagct tataaggcaa
    28381 gtccctcatc gttaactttt gttgagcgca aagggcagcg gccgttccgg cggcttgccc
    28441 ctgggccatg gaacaaactg tgttccgcgt ggacatgtgg gcaacataat ccgacgtaat
    28501 caacatccct gcacaccaaa gattgtcgag acccgagacg caaagggcac gatacggcat
    28561 gccatatgtg ccgccatcct tgatttgata gcggggcgcc atgtcatgaa aaccatacac
    28621 gaagacctcg tcggggaagt gacgcccttc caacacatcc tcacgcgtta cgtcgtaatc
    28681 gcagcggatg caacgagcac gccgaatatt caaccgtggt gcggtcctcg cgataaacgc
    28741 cttttcgcaa cccgggacgt atttccggaa gagttccaca gcacgggcct gccgtcggcg
    28801 gagctctaac tctgcccacg tcactgcatc tcgattcgtc ggactgacgg ggagcttcaa
    28861 attgagctta atgaacatga agtaattgtc atgaaccgtc gtcgttacgg tggacatgcc
    28921 gatttcagtc gcttcccgac ggaaatcttc agggagattg acaagtcgtc cttcaagtcg
    28981 cacgatctgg ccatcttgac cacttcgtaa accccgggcc agttcggtca acgcgtcatg
    29041 gctctctaaa aactcgtagt atttctccac gttgacattg gccagaccaa tgctattcgc
    29101 cacagcgtaa tcattaggtt ctgtgaaatc tgccccggca aatgcggcca aatctccgta
    29161 tgctgtacaa tccacaaaac tatgagcaaa aaaagcctcc cgaccggccc gactctccgt
    29221 gaccgctccc aagagtctat ttccgtcgcg gatagcccct acgaccatcg tgttgagaaa
    29281 catctcaacg gcagcctcag ctagcatctc catcgtgacc agcttgtaca tctccgtatc
    29341 aatggctgtg cataccgaat cgtagttgaa tcccttctcc atttcggcgt ggcctgaaca
    29401 cgcccccagg gataggagcc ggtcgatgat cttctgcggg aatccccgga taacctgcct
    29461 tttttccaca cctggaaagg ctttccaaag attgtagaaa ctgtggaggg cggtaccccc
    29521 ttccgtaacc gtaccgcccg tataaccctt agcttcgatc aaggccgtct tcacgccttg
    29581 agccgccgcc gcgagtgccg cgacgacacc agccgttcct ccgccggcga tcacgacgtc
    29641 aaatcgccgg accggcaatt ttttcggcgg ttccatgtac caatcgcctt cttgaggcgg
    29701 agcttcttcc cctcgcgaag tgctccccag aaaactcgcc agaccggcag ttcccaatcc
    29761 aatcgcggcc tgatgaaatt cgcggcgttt cataaataac caacctcgct gtgaaatccc
    29821 gtcacagtct cgtaaagatt tgaccttagt ttaagagctg gtaggggcga cttatagctt
    29881 tagagccaac tcggcgatgt ggcggcccag attctgggca gttttaacac cgaattcgtc
    29941 ggtagatatg tcgtcatttc cgccattgtt ccacaatgta gccccaaagt gacctgtcgg
    30001 acgaccatca cccacgattg ccatttcatg acacatgagc gcccgatgga tcgaatccaa
    30061 cacatattct tggccaccgt tgcgggcagc ccctaccgcc aaagcgcccg ccagttttcc
    30121 agacagcttg aactcctttc ggaacacctg gccgagacga tcgatgaaag ccttacacag
    30181 cgtactcatg ttagcgtagt gtacaggcga acctacaatg agtgccttca accgcggatc
    30241 ggcaatctgc tcggccaccg ccggaaaatc gtcgcgctcg ccaggtggca aagggattcc
    30301 caatgcgggc tctacgggga tgcgcaaatc ggccagttca atgaggaccg tctcaatctt
    30361 ttctggagcc accgctttcg cggcatccaa cacaatcttc agtccttgag cggtggtttt
    30421 gcctttccgt gggctgcagg acaccgcaac aattttcacc gtcttcgatt gacgttggga
    30481 ctcagcttcc tccgccagca gagatccccc taagccagcc ccaatcatag cagaacctaa
    30541 accaacctga tgcaaaaaag cgcgtcgttc acatggctgt gtcatgtcat ctctccttcg
    30601 gaagcctacg cgtctgaacc cctggtcttc accaacgaaa gcaccgtgcg acgcacggac
    30661 cgtcccccct tcgtcggaac acgcagcatt ttagtcagcg cactgacaat tgcaacagtg
    30721 gtcccattgg agagacgccc gctccagaga aaacccacat actgtcgtgg cgtgctatcc
    30781 tggtagtcca ggcgctgtcg tggccgcacc aaatgacgcg gtaaccgttc ctgccacatg
    30841 gaacctaacg gtgtgcgtcg cttagcgttc ggtttccctc cggaaacgga gctatttccc
    30901 gaatgccaaa atgacgtggt aaactgccga cggtccagat ctcttccaaa tcagccgtgc
    30961 ccggggcggt cactcgcgaa aatctctgga aggctttcct gtcaggcttt gtcgaagggg
    31021 cctactcggg gtagacatta cgaccatgtt gcggccattc cacccaaatt gcgggcggct
    31081 aatagaacat ctgggggcat tttctttttt caccaagttt ccggtaaagt ttgccgatta
    31141 ttccgacagc ggcgaataca gagagagtat cgcttaatcc ggttgtacgg gttcagtgag
    31201 ccgttccgct ggaactcaaa aggatgttac ccgaccatgg accccctgga tcagaccaga
    31261 aaatgcctgc gatacgctac cagaaaagat ccccgctccc cgaagaatca attatcatgt
    31321 ttatcgcata agtggggatt gggatcgctt gtctgtttct gcaccctcgt ggcgctcctt
    31381 ctcgccacgg gctgtcgcac catggacttc tacgaccgtg ggctggaaca gccggcccct
    31441 acccaggaat tggaacctcc gcgagagctg tccaaggtct ccctcccctc ctatcgcctg
    31501 gaaccgcccg atgtcgtcca gatcgaggtc cccaagatgg tccc
//
LOCUS       DAOSUT010000027        31540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_981_length_31540_cov_9.226806, whole genome shotgun sequence.
ACCESSION   DAOSUT010000027 DAOSUT010000000
VERSION     DAOSUT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 31540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31540
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_31540_cov_9.226806"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..716
                     /gene="nadD"
                     /locus_tag="PLD05_03955"
     CDS             66..716
                     /gene="nadD"
                     /locus_tag="PLD05_03955"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOP76616.1"
                     /translation="MKLGIFGGSFDPVHYGHLLLAESCREQLNLDKVFFVPAGIPPHK
                     RETRLTPAIHRLAMLELAIAGEADFEICRYEIDRDEVSYTVDTLRWFRDQFPEAELYL
                     LVGADMLNDLPNWREAAEICRLALPVGVHRGGAAPPAPERLLPLLSSERLEQFKKTRV
                     DMPLIALSASEIRRRVQHGQSIRFRVPKAVEQYILHQGLYRNSTPEELTNSSQGGC"
     gene            complement(756..1178)
                     /locus_tag="PLD05_03960"
     CDS             complement(756..1178)
                     /locus_tag="PLD05_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76617.1"
                     /translation="MNLSSAGAGRGLPAAAQPDRKDPARMVKARKNIDGKGVSARLAF
                     GTIARFSQVSLVRRAVMEGQDIIIRVDAMKKELQELGCEIREDWLGGKDCTLCEIRGK
                     RIVFIDLESSPQELSASLARLVAELKATLIGQAASTDV"
     gene            1212..2135
                     /locus_tag="PLD05_03965"
     CDS             1212..2135
                     /locus_tag="PLD05_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76618.1"
                     /translation="MQNCKGAPVEQNPADLPLQQQIAVLKERLAHAQRYTALGELVST
                     TTHEFNNVLTTIINWAKHGLRHKDTATRDKAFEAILNSALRGAKITQTILGMARKRQN
                     TLEPTDLQQLLADTLLLLEREMNKYHIRVEVFAQPVPPVMADGNQIQQVLLNILVNAR
                     QAMPRGGRVVIRLQHEPDSPFVDLQIRDFGTGIPPDVLPRIFDPFFTTKSGPDASGKG
                     GSGLGLSACRDIIEAHNGRIRVESAVGKGTCFTIRLPVATQNASSQSAGAAGNEVGNH
                     ISPQAASPAPPVFSVSDVNQTHCSASLSPPT"
     gene            complement(2214..>2810)
                     /locus_tag="PLD05_03970"
     CDS             complement(2214..>2810)
                     /locus_tag="PLD05_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76619.1"
                     /translation="SPPPVLSCYYPNPIFVRAKNPEQFWEALVDIIDDDFEIESESPV
                     RQLQTVFTSGRIDTRPKVAATILEPWHHDSPDRYSRWEATLQSLRRRAEVRVDPRDGG
                     YWITVLVFKELEDVNQPLAATTSSATFRNDSSLTRVISPIGEQQVNAGWIPLGRDTAL
                     EQRLIEKIAARFGAAPTTETAPPLVDIPSLGSPPVPTP"
     assembly_gap    2811..2910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2911..3290)
                     /locus_tag="PLD05_03975"
     CDS             complement(<2911..3290)
                     /locus_tag="PLD05_03975"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76620.1"
                     /translation="METAKQISVKLVNKPGRLAQLLDAMAKAKVRCRALVVMDSGDRG
                     TVRFVPTEWERAKRVLEELNLPYELADVLLVDVPKQPGGFRKICERLAEHHLNIDYAY
                     HSFGSELGAKGGGVAVIKVNDLNKA"
     gene            3760..>4211
                     /locus_tag="PLD05_03980"
     CDS             3760..>4211
                     /locus_tag="PLD05_03980"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOP76621.1"
                     /translation="MLIIETPGARLPRPIIWPPYPPPHPPIPIPPPRPVPPPPEISYS
                     VESLSVNGRINNQVAQVEVAQTFRNTGSRTLEASFVFPLPYDGAIDRMTLMVDGREIP
                     ARLLKADEARREYESIVRRNRDPALLEWVGTGMFKTSVFPIPPGQSRTV"
     assembly_gap    4212..4311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4312..5868
                     /locus_tag="PLD05_03985"
     CDS             <4312..5868
                     /locus_tag="PLD05_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOP76622.1"
                     /translation="SAKLLSYKSPDEEDGFFLLLAAPQIATQDGKPLPKTVMFVLDRS
                     GSMSGKKIEQLRQAMQFVLNNLREEDLFNIIAYDSDVEAFRPELQRFNDKTRREALAF
                     VEGLVAGGSTNIDAALRTAFSQLQDDKRPTYIVFMTDGLPTTGVTNEMQIVENAKNAN
                     TVKARIFCFGIGYDVNSRLLDKLARTGRGYTEYVRPDENLETRISSFYRRIEAPVLTD
                     VTVDVVFDEPPGPPPVYRLNPQPPFDLFAGEQLALVGRYRKSGRAEVVLKGRIDDEQK
                     EFRFPVDFVARSGDESKAFVEKLWAIRRVGDILDEIDLRGKNPELIDELTRLAMKHGI
                     VTPYTSFIANENVPIHDLTQSRRETESRLRALEAAEGYGGFAQRSFKGRMQQAQAADA
                     ASALRSFGGLGAEFAAPSAAAPGGAGMMGGVAASAPALAPAEEEIARAATGETMRYVG
                     NRVFYKRQGQWIDGTLKPEQQQNPIRIKQFSEEYFNLVRQYGRQVSQYLVFDEPTLVN
                     INNQAYLIEP"
     gene            6132..7133
                     /locus_tag="PLD05_03990"
     CDS             6132..7133
                     /locus_tag="PLD05_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP76623.1"
                     /translation="MSGRIPLRALSELVSDEENDTFALLSSKEELTTRDGKPYWMVTF
                     RDAQVEFTFPIWCDSPWAAACREQWAVGTFYKLRVVRRETRYGPQLEIHRIRETVDQD
                     RADGFDPLMCLPQSRFPAEELFQRLEALVQKEIKSPSVLQLLVMILHTFRESILTLPA
                     ARSHHHAYVGGWLEHTLSVTETCVFLADKYIQLYPDMKPPLDRDLVIAGAVLHDIGKL
                     RELEFQITTTEYSPEGALLGHIVLGRDFVRDFAPKAGLDSEQQLRLEHLIIAHQRLAE
                     WGSPRPPMTPEALIVHFADDLDAKFHMFWKALQHDSKEGPLTSDRNPLRQAIFRGLK"
     gene            complement(7173..8048)
                     /gene="prmC"
                     /locus_tag="PLD05_03995"
     CDS             complement(7173..8048)
                     /gene="prmC"
                     /locus_tag="PLD05_03995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOP76624.1"
                     /translation="MTQAEVWTIGRLLRWTTEFLQQRGSETPRLDAEVLLAHAHGWER
                     IQLYTHYEDVPGEEVRSRFREMIRRRAEGVPVAYLVGEREFFSLKFRVTPDVLIPRPE
                     TEFVLVTLLERARQSGQGGWRICDVGTGSGVLAICAAKHLPDSQILAIDKSPQALAVA
                     KENAIRHHVADRIEWLESDLLEAVPSEMTFDFIISNPPYVSEAEFANLPTTVRNYEPY
                     QALVAGPRGTEVIERLIPQAAEKLVTGGYLIFEISPMICQRVCELVEADGRFRLGPVV
                     RDLARLPRVIQAQRI"
     gene            complement(8316..9389)
                     /gene="prfA"
                     /locus_tag="PLD05_04000"
     CDS             complement(8316..9389)
                     /gene="prfA"
                     /locus_tag="PLD05_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOP76625.1"
                     /translation="MRKELDEKLARFEELERLLHDPAVLSDPSRMATIMREHGSLAKV
                     AVKYRKFKEINQQILELRQWIEGEDPELRDLAEEELPRLRQEREALWDELLELALGGE
                     DANRTRCIMEIRAGTGGEEAALFARDLFEMYRRYAESKGWKLEILGMNPTELGGFKEI
                     SFGLEGEEVFRRLQYESGGHRVQRVPETEAKGRIHTSLATVAVLPEPEDIEIDIKPED
                     YRRDTYHASGPGGQHVNKTSSAIRLTHYATGIVVQCQDEKSQHKNFAKALRVLKTRVY
                     EYYRRAKEAERAQERRSKIGSGDRSERIRTYNFPENRVTDHRINLTLYQLDQIMEGNL
                     DPLIEALLDYERNELRLTFGSLD"
     gene            complement(9539..9763)
                     /gene="rpmE"
                     /locus_tag="PLD05_04005"
     CDS             complement(9539..9763)
                     /gene="rpmE"
                     /locus_tag="PLD05_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOP76626.1"
                     /translation="MKEGIHPKYVECKVTCGCGNSFVTRSIKPELKIDICNVCHPFFT
                     GKLKYVDTTGRIEKFKSKFAAGYSSVGKKK"
     gene            complement(10142..11785)
                     /locus_tag="PLD05_04010"
     CDS             complement(10142..11785)
                     /locus_tag="PLD05_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76627.1"
                     /translation="MLFVFLGLCLAGCPRPRQPETTSDTTSAEQTATLHRDELFEIAI
                     ANLNRLHQFDGTDMALQVADRLDQWIKTQPPLKEWEVDPLIKTLPEGFVNAELLQDLE
                     KLSLDREDGLFLQSAVWLRDIAAWARGEDPSELARACRLFDWVVLNIQLDSQDQARQL
                     PSGDLLVQKPWETLLLGHGSAIDRAWVYVLLCRQEGLDAAVLSVNRAPAGAEPRWDMW
                     AVGVLINGKIYVFEPALGMPIPARDGVQVVDGEGLVITPATVDELAVDDSLLRRMDAD
                     PRNPYPVRASQLEQVMVYVEGSAPYLSRRMEMIQRMLSGEESLVISAHPSDLAMRFRG
                     LAHVRDVQLWALPYLVEKQRKEFEGVVAKYLFNQIAPFMLTSETGIMPLWRGRILHLR
                     GNLVGEEGAAHYYQLARPPERLIDSGQLAADEQRMYTIAKLNASYWLGLIAVDQGNRK
                     SAEDYLINRTAMVLPDQPWHSGVLYALGRLNEKLGDYSQAITTYRSEFQLPNRFGNLV
                     RARLIEELTGAKPLEISPEPTTNKADKASQPDKTSGKQE"
     gene            complement(11887..12717)
                     /gene="kdsA"
                     /locus_tag="PLD05_04015"
     CDS             complement(11887..12717)
                     /gene="kdsA"
                     /locus_tag="PLD05_04015"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOP76628.1"
                     /translation="MPNRIVSVGNYRCGPGESLLLIAGPCVIESEPLTREVAFFLAEF
                     SKSRGIPIIFKASFDKANRTSLHSFRGPGLSQGLAILARVREESGLPVTTDIHEPAQA
                     QPVSEVCDLIQIPAFLCRQTDLLLAAAQTGKPVHVKKAQFLAPWDMRYVIEKLEGAGC
                     QNILVGERGTFFGYGRLVNDLRSIPILHDLGVPVIFDATHSVQEPGGQQGRSGGDRRM
                     VAPLARAAVAAGADGVFLETHPRPDEALSDGPNMVPLADLPRLVETLSELREWLKERI
                     "
     gene            complement(12710..13741)
                     /locus_tag="PLD05_04020"
     CDS             complement(12710..13741)
                     /locus_tag="PLD05_04020"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOP76629.1"
                     /translation="MEQDRLPVDSRLVVVLHNPVAGRRPSDRRIEQLVNALREKGFSL
                     HVSTNLSQATDLANREFEKGNLRCLVGAGGDGTASELANRVQPGVPVCFFPTGTSNLV
                     ASYLGMKKRIPFVVSLIETGATVCLDAGLANGRIFLAMVSCGFDAAAVEMAHRKRVAG
                     HRQGRGGFSGFIIPICRLINTYPFPEMTVEVSDGYEGGSSGEPSSSQRTIKGRWVFAF
                     NLPKYGWGLPIAPQANGMDGLLDLCVYGGGGFWRGLWYTVTTQVGIHERVCDFVRIWG
                     TRFRVTSNEPVPYQIDGDPAGYLPLDIGLAPGRVRIVVPERTAEMLQKQRRQSESLSR
                     VKNGSLVCA"
     gene            complement(14382..17732)
                     /locus_tag="PLD05_04025"
     CDS             complement(14382..17732)
                     /locus_tag="PLD05_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOP76630.1"
                     /translation="MGAAARYEIIGTIATGDFATVYRGRDRELGRDVAIKEIHPHFRR
                     DPKQLERYWREAQLLASLQHPHVITIYDVVKSRGWLILELMRTSLQKSIEKGPIDLDF
                     LREILIGSLEALDFLHRNGIVHGDIKPSNLLLDNQGRVKLGDFGLARRATDEHGSLLK
                     GTTKYMAPELISAQFGPVGPASDLYSLGFTAYELICGPDFELLFPTLSTFGRDKQIAW
                     MMWHASADQKLPPIHRVLEGVPDDLAEVVDRLVQKNQQDRIPSAAVALQRLKRPAAEV
                     PPPPVSEEASEEELAERKRKRATRMIAIAAVACSLVISALMLWPSQKQSPPPAVSVEP
                     LSGRVENVLLDARLLIVDRGALGKEEIEIRAWDEVFVNDKKSLLRDLQPGDQIKITVL
                     RDDQGRQIRRIEALRPERFAGRIEEMSPEMGTLSVTGSPGEPKLVVRVPNTAAIRLNG
                     NETIDGKTLGLADLAVGDRVELEYQRSENELIALKVDAYRVMRSKGVVEVVDASKQSI
                     RLALSDSGKTLTLPWAPDCVVTVNGLTAIGGQLQKPTDLLPNDQVEIEHDDQIRRIDA
                     YRVLGVAGVIQQVHFDANAIDVQLTGDGKVAQFLVPDTCTITIAGSPATIVDLRPGDQ
                     VDIQHDTPGTTTGRALKVAAIRPADQTRFALLIGVEEFDDQNIPAARGIVEGVRRLAD
                     VLIQRGAFPRNQTLVLTNPSRVRVEQAVSSFLKNVPGNADVVVYYAGYAAKDQEGRVF
                     LLCKESHLQQLATSALSVQFLADSLEQASAKSKILLVDVSPEKAKASGQAFVSAEEAL
                     KAVQGPPGMAPFRTVTAIAACAEGQEAMLAGDGTVFGAALIEAYQGKADENRDGRIEA
                     GELYGYLSRQVPQLAQDKQRPRIFLPDNRPPRLSEEAKTAIRQLAAMVLQENLDSRVV
                     NDIYRTALKLAGREPEPRVLYALIQIKMGQTADAQRVLETLKLERPRELVAYLGLAWV
                     KLVRRDYNGSVNELNAFVQNWEASQVKTDPGQIVTQLDGSKVFHFIGQLREFAERVSE
                     DAERPSSVVFQNLDNGVKQLGESLAQAYTDGRTQIKELLDRFDQELSRAADQATAVKL
                     RYERRQLGRYTAFPFEEISRQVIAGLDR"
     gene            complement(17822..18811)
                     /locus_tag="PLD05_04030"
     CDS             complement(17822..18811)
                     /locus_tag="PLD05_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76631.1"
                     /translation="MPIKIHCPLCGTEMTVGDTALDRTVGCPWCRHRFRADAVLAQGA
                     KPTSPKGEVPLATPPTAPKGAEPAKKPARTGETPGPVGRVATPDGAAQPVSKEEARLS
                     KPVSVGPGQPSPGKTAGRDEGRGVLADKAGQASERAAGVAPGKPSPGESPAGGDTSLT
                     MPRRRVARLILTETAEPKWTLAPDGSLPSLHLEERSDEERGKEKRRSSNPLLLILVLC
                     SSLATSLILLFVDTSPTTQGSPEEKARARQILAAEYYSHLNPNAPLAEYQRLLREAHW
                     AHSRGDYKREKELYRQVLLLLYAERGRNTYTGLTGSRTRDQQLEELLRILLKD"
     gene            complement(19169..20101)
                     /locus_tag="PLD05_04035"
     CDS             complement(19169..20101)
                     /locus_tag="PLD05_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP76632.1"
                     /translation="MEFGEFQHSQSLLLVACFMWGLAAFLLTRWFIQVLQTEDLQQGA
                     EWRYDVSRINELRRQDRFFRLFQPLIQLLARFNRAAFRDALPEIQRHINCAGLPRFWL
                     AEEYLAKLELIAFLLLPIYVYVAVRAVGAGGILIAVVFAGLTVFLLRLRLADRAGRRL
                     QAIKRRLPFLLDLLTLLMEAGSTFLNALAQAVEEYRGHAIAEEFGRVLADIRMGKTRY
                     EAFSAMRERLADDEITSIIGSILQGETLGTPLASIFRTQADVLRIKRSQRAEMIAGEA
                     GVNMLLPGILVMAAAVLILVGPFLMNYLYFGMSL"
     gene            complement(20191..21045)
                     /locus_tag="PLD05_04040"
     CDS             complement(20191..21045)
                     /locus_tag="PLD05_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP76633.1"
                     /translation="MSDNRDLLFLGSLATGLAIALIVYGGRETIAQAFLKMEADIAEK
                     LRRLRVHTRHLRFWLLAWLGGVVAAFVGLTFVVGSLIFALIAAVFLLAVPWYLLRRLA
                     ELYHRKIEDQLADAMVTLANAVRAGLSLAQALEILAAQCPRPINAEFRQIVAEYNLGK
                     PLDRTLIEAKERLKSENFALFAAALLASHEHGGRLNETVERIAGSVLELQRLERKVLS
                     ETAQARKSAIYMAFAPVVFLIGFYFMDPENTMLLFTQPLGHLLLGMAIILDVVAYLWA
                     RALLRPDI"
     gene            complement(21047..23023)
                     /locus_tag="PLD05_04045"
     CDS             complement(21047..23023)
                     /locus_tag="PLD05_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOP76634.1"
                     /translation="MKIWFNRINESHRTMIEVDAAEVTIGRDASNTVVLASPLVSRRH
                     AIVRCLEDGRLQLENLGLNSCLVGDEEVLGGQTVVFSPGTRVRIWPYTLTFEAEKAVN
                     ISRQELEAHLRSLVADLELRIHRKLLERLDLYEFENRPTNDPQSILLLENNIEDICRE
                     LRVFEPENEPILEEITGLMLRDYLVNSLIMESGKDSLFEMTGLNANEFDIPATLVPER
                     ETELAGILNFIRERLRLSECRDTTEQVQRIEEHFNVVFQLVRPHLHAELRKYLILRTL
                     KKDLKDTIFGFGPLQDLLRADSITEIMVVGKDQIYVERNGVIEKSGRRFISDKVTEAI
                     IERIVAQVGRRIDKSQPLVDARLPDGSRVNAIIPPLAVKGPCLTIRKFPVQRMTMDDL
                     IEMGSISKAAATFLRASVIDRKNILVSGGTGAGKTTLLNVLSSFIPFKERIVTIEDTV
                     ELRLHQEHVVTLEAKPPNVEGQGAYTIRDLVRNALRMRPDRIIVGECRGPEALDMIQA
                     MNTGHDGSLTTLHANTAREVIERLEVLILMAAELPIYSIHRQIASAIDLIVHITRLPG
                     GRRVITEITEVVGVDPETKNVILHDIFNFRNGVSLEPTGYLPTFIDSLVEKDLLDLDF
                     LYGKDDHQKEMFSMAEAVGGPAGSGPLEEKRLRI"
     gene            complement(23094..23864)
                     /locus_tag="PLD05_04050"
     CDS             complement(23094..23864)
                     /locus_tag="PLD05_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76635.1"
                     /translation="MKGIHGVVLGVAVGIAAALFNWAYITRATGDIDAVAFVGIAPGK
                     DLEAGHILAEEDLVPVPIPAIPARRLKDFGYLYADRASIVGLRITRPIPGGSLLLQDD
                     LRPPPPKLRFGGQPVGNTEEIAMFIPIDVRTIVPSLLEPGDMVSFIVGGPTPTAGVGN
                     PDPAAGGEGSAAGSSLSGGPQVIGKFKVLAIGNRLSSVEVHRAYQLPQGQENILTISV
                     RLEGGRLEPKAERLWKMLELTNFRQVGVILHSREASGN"
     gene            complement(24013..25116)
                     /locus_tag="PLD05_04055"
     CDS             complement(24013..25116)
                     /locus_tag="PLD05_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76636.1"
                     /translation="MAGDHPSHPEIPTELERKDSPQTDGVNLSPSRALAVDSELGWSK
                     LAKRITGWTSNLLVTFLILAAGLVFGVQVLLWWKDTATEDQTTVNRDTRESLQVSRRW
                     AEFYGGGGGFSCISFQGEEKAAIRRCEETCLEAVQRAVVRPGLPDAAERRVLAKLASI
                     LPIREEPNGSRVYRYPGDVPLWVGVKLGEKKSEGTDRAGAVDTDATDAGSLPPGSRIV
                     AWAFLMPVTEREWTIYLLSPAAEGPQKGTLGSLGEEEFLFPVPPGATVVSRLSPGEGA
                     FFGAFEESTPGASVTWPLFLDRELERQGWVPVGDWLGGAARKRRVFGRGTQTTEQSFL
                     SIEITQDAHRPARGLVFGFRWTTEKRERSSEPQ"
     gene            complement(25106..26173)
                     /locus_tag="PLD05_04060"
     CDS             complement(25106..26173)
                     /locus_tag="PLD05_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76637.1"
                     /translation="MTKFSPLRWAAEVNCSQSLPRGGTVFGKRGLAPLEFVIAVPLFL
                     FIMALMINFGTVSAWRVRGLAVARQAVWADRPPRNGGTVPRPDYWPIPANLGAGADYD
                     APVLDDPRVNLPVARGPTLGTFVVNDHLLDPTKGFRRGTSEIERSFPMLASLGGYQLS
                     SRAPILDNCWRYRQTGLPYWWHDHWAHRVTALYRLPTAGGNYLALYVQAALAILRMPQ
                     RNDLLILDRDPEFPAYAARFGWRGGGSPDFHPGLSHFCSLDLSVAEERVENLIDRIAG
                     VSPKQGPPQVNHVPSLAERMAGAYISLYRRVIQELQNQLNAVPPPSASQIAAIQAEIA
                     DLQQKIDTLEAFRQSLQNHGR"
     gene            complement(26220..27686)
                     /locus_tag="PLD05_04065"
     CDS             complement(26220..27686)
                     /locus_tag="PLD05_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOP76638.1"
                     /translation="MLSKGDVRTIAVMRKCAARLGCRLRSLSADQDGAMSVVSVFAVL
                     LLTILLGMVMNVGRVVDHKIRLQNAADAVAYSGGVVIARGMNALAFTNHLLCDVFALT
                     AYMREARDRHAESYVPAILDAWNQEAPVFAQSNFEKFIPLASAIPAKTPLEQALVDGF
                     CSWAAAASPVILPTLEFILQEELIPEYQRAVVAAFPDIAQQAAMEVARRNGRPDFGRG
                     EMLGVLWRTNVVPVGGPGEFVQRTLPVVDPVMDQFPNQADYFNTAVEQRRNLSHHYLR
                     LWNNRSLIFFDREAKMSQFASLWRSFTCGQLEQLLSEYPASNLPFVIQTPGDEVVDPN
                     SHLDQYFTFVGVAYWRPMRATLPGLFQHPMGSDTIAYAQVRVFIPRARLIWHYYVDTP
                     ENILGGIPGDFAELPPDDAEQSEPAQGEWRVVREDVPTHWDLLNQNWTCALQPATVWS
                     LPLIVQTKPPLPEFAGWNVQLPSLPGWTTADIQYVSPH"
     gene            complement(27807..28793)
                     /locus_tag="PLD05_04070"
     CDS             complement(27807..28793)
                     /locus_tag="PLD05_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOP76639.1"
                     /translation="MLRATLIACLPWAGVLLAACAVAWLLLRAQKPQANFSRLLTLHR
                     DEAGAVQSLSFVLTLPFFVLLMLFIIQVSQIMIGTVVVHYAAYAAARAAIVWIPAKVG
                     DWELENCIGPGYVLDPNAPDQVIPITDPTSSEYGPTSGGLTFIIPYDPASPKYQKIVN
                     AAILGVMPICPSRDLGLTVSGSLGTAAQLLQQAYLANVPGASSIPRIPRRLENKLAYA
                     RLATELELRFFHSNREPPLAPYFLEDDIYEFRPNELGFQDTVTVTLTHHMALLPGPGR
                     FLARPVRRTDEVAEQIERRNGIATYPLRASISMGLEGEKSVIPYTYSIVAFW"
     gene            complement(28838..29419)
                     /locus_tag="PLD05_04075"
     CDS             complement(28838..29419)
                     /locus_tag="PLD05_04075"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOP76640.1"
                     /translation="MLGILLILLAVASMTDVLWGRIPNKITYPGIISGLLLNVLGTLA
                     VRLGWARAEHLSALGWIGMGQSVLGFLACGLVMLACYVFFRIGGGDVKLIAMLGTFLG
                     PDRGITAMLWTFVLGACLGLIALVWRVGPWRLLVLAGRHLLWSIRLGRWDPLTPEERA
                     QLQPPLFLAPSALAATVIVGFELVERYVLAVGP"
     gene            complement(29493..29699)
                     /locus_tag="PLD05_04080"
     CDS             complement(29493..29699)
                     /locus_tag="PLD05_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76641.1"
                     /translation="MKKASLWQLLRQIHADDRGAVSLETILIIGAIALPILIFLIKVG
                     WPRIKGYFTQGLQELEAERANAIQ"
     gene            30501..30956
                     /locus_tag="PLD05_04085"
     CDS             30501..30956
                     /locus_tag="PLD05_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOP76642.1"
                     /translation="MRAALVVAIVLLGMNPIGRGSEPVSYSEQFAYSAAYRQSLETQQ
                     PFLVIVGAQWCPACRKLESEVIPKIKEMGLTKQVAFAVVDYDAETKVAQQLTRGGPIP
                     QVLVFRRGEQGWKSDRLVGYPSQKAVISFIQAALSSKDQAPAGQQASNN"
     gene            31013..>31540
                     /locus_tag="PLD05_04090"
     CDS             31013..>31540
                     /locus_tag="PLD05_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOP76643.1"
                     /translation="MLPPIERHNIRQLEELNQRGGRMLTVVDLIEAGTISPEMAAAVM
                     EALARGASILTGARPSGAGKTTVLAAFLNLLPPDVTIVTVDDPAVVGQALARPPEHPT
                     CYLVHEIGAGHWYGYLWGQAVSDYLRLTRGPHRIASCLHADTLEELTHILTSPPLCVD
                     PSDLHRVGILGFMRMD"
BASE COUNT         7071 a         9413 c         8050 g         6806 t
ORIGIN      
        1 ggtttcgtgt cgattctcgt gttcccacgc ttgcagaata aaacacagta tcgccaattt
       61 gaacaatgaa attaggaatc tttggtggta gttttgaccc tgttcactat gggcatctgc
      121 ttctcgctga atcttgtcgg gaacagctca atttagacaa ggtattcttt gtccccgccg
      181 ggatcccccc ccacaaacgg gaaacccgtc ttacgccggc catccatcgc ttggcgatgc
      241 ttgaactagc cattgcgggc gaagcagatt ttgagatttg ccgttacgaa atcgatcggg
      301 atgaagtcag ctacaccgta gataccttaa gatggtttcg cgaccaattt cccgaggcgg
      361 aactgtacct cctggtcggt gcggatatgc tcaacgattt gcccaattgg cgcgaggccg
      421 ccgaaatttg tcggttggcg ttgccggtcg gggtgcatcg cgggggagcg gcccctcctg
      481 ctcctgaacg gttgttgccc ttgttgtcct cagagcgtct tgagcagttc aagaagaccc
      541 gtgtcgatat gcccttgatc gccttatcgg cctctgagat ccgccgccgt gtgcaacatg
      601 gacagagtat ccgatttcgt gtccccaagg ccgtagagca gtatatcctt catcaggggc
      661 tttaccggaa ttccactccc gaggaactga caaactcctc tcagggtggt tgttaggcgg
      721 ttgttgtttt cagaagccca gccgacagcc gagtattaca cgtccgtcga agctgcttgg
      781 ccgatcagag tagcctttag ttccgcgacg agccgcgcga gtgaggcgga caattcttgg
      841 ggtgagcttt ccaagtcaat aaaaacaatc cgcttccctc gaatttcaca gagtgtacaa
      901 tctttcccgc caagccaatc ttcccgaatt tcgcaaccca gctcttggag ttcctttttc
      961 attgcatcga cccggatgat gatatcctgg ccttccatga ccgctctccg aactagggaa
     1021 acctgggaaa atctagcgat tgtaccaaag gcaagtcgag cggagacacc ttttccgtcg
     1081 atattctttc tagctttgac catccgcgcc gggtcttttc gatcgggctg agcggcggcg
     1141 ggaagacctc gccccgctcc cgcggatgac aaattcatat ccaccatggc ttaggcagtg
     1201 tatcgacagc catgcaaaac tgcaagggtg ctcctgtgga acagaatcct gctgatctcc
     1261 ctctgcaaca acaaatcgcc gtgctaaaag aacggttagc ccatgcgcag cgctacacgg
     1321 ccctgggcga acttgttagc accactacgc acgaattcaa taacgtcctc acaacgatca
     1381 tcaattgggc caaacacggg ctaagacaca aggacacggc cacgcgagat aaggcctttg
     1441 aggccatcct gaattctgcc ctccgcgggg caaagatcac gcaaacgatt ctcggcatgg
     1501 cccgcaagcg tcaaaatacc ttagaaccga ctgacctcca acaactcctc gccgatacgc
     1561 tccttttgct ggagcgggag atgaacaagt accatattcg tgtggaggtc tttgcccaac
     1621 ccgtgccacc tgtgatggcc gacggcaacc aaattcaaca ggttctcctt aatatccttg
     1681 tgaacgcccg gcaggctatg ccgcgcggcg gccgtgtggt cattcggctc caacatgaac
     1741 cagattcccc gttcgtcgat ctccagatcc gcgacttcgg gacgggaatt cctcccgatg
     1801 tccttccacg gatctttgat cccttcttta caacaaagag tggtccggac gccagtggca
     1861 aaggaggttc cgggctggga ctgtcggcct gtcgagacat cattgaagcc cacaatggcc
     1921 ggattcgggt agaaagcgcc gtaggcaagg ggacctgttt caccatccgt ctgcctgtcg
     1981 ctacccaaaa cgcttcaagc caaagcgctg gggcggcagg gaacgaagtc gggaaccaca
     2041 tatccccaca ggcggcatcc ccggcaccac ctgttttctc ggtaagtgac gtcaatcaaa
     2101 cgcactgctc cgcctcgttg tcgcccccca catgaggtct tcgcaacttc gtacctggca
     2161 ggagtttgca gaatagctgc ttcaacctct cgggccgcgg gatctcttgc ttttcatggg
     2221 gtcggcactg gtgggctacc caagctcggg atatcaacca agggcggggc tgtttccgtg
     2281 gtaggtgctg caccgaatcg ggcagcaatt ttttcgatga gtcgttgttc aagagcggtg
     2341 tccctgccca agggaatcca ccccgcgttg acttgctgct caccgatcgg gctgatgacc
     2401 cgcgtcaaag aactgtcgtt gcgaaatgtg gccgagctcg tcgtagccgc caagggttga
     2461 ttcacgtctt cgagctcctt aaagactagg acggtgatcc agtaaccccc atcccgggga
     2521 tccacccgga cctccgcgcg gcggcgaagg ctttgcagag tggcctccca ccgcgaatag
     2581 cggtctggcg aatcgtgatg ccagggctct aagatggtag ccgcgacttt gggacgagtg
     2641 tcgatccggc cggaagtaaa aaccgtctgc aattgccgaa ccggtgactc gctttcgatc
     2701 tcgaaatcgt catcaatgat gtccaccaaa gcctcccaga actgctcggg gttctttgcg
     2761 cggacaaaga tgggattagg gtaatagcaa ctgaggaccg gcgggggcga nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccttgttga ggtcattcac ctttatcaca
     2941 gcgacacccc cacccttggc accaagctca ctgccaaacg aatggtaagc atagtcgata
     3001 ttgagatggt gttcggccaa tcgctcgcag atcttgcgga atccccctgg ctgcttgggc
     3061 acatccacga gcaagacgtc cgccaattcg tagggcaagt taagctcctc aagcaccctc
     3121 ttggcacgtt cccattctgt gggaacgaat cgcacggttc cccggtcacc cgagtccatg
     3181 accaccaaag cacgacaccg gaccttggcc ttggccatgg catcgagcaa ttgggcgaga
     3241 cgtccaggct tgttgacgag cttcactgag atctgcttgg ccgtttccat cgtttctccc
     3301 tttcgttgaa aaaccgggtt ggccaacaac acccctcacg gagaaggctt acagggcccc
     3361 tcccgtgcca cggaaatcac ttgatcgata tcccatggcg cgggcggaca cccgtaaaaa
     3421 ggatacgcta caaggcccaa gcaagtcaac aatctgaagg acattcccca gcggacggag
     3481 gttcatccaa ttgcgagggg gaacttttcc ggggttggca acgtcagtct aatgaagacg
     3541 gtcctgcgct actgcaccga ggtgcctgac tccactcgtc tgaaaacgca ggttcgtaac
     3601 cggatgtggg tccgctcgac cctacgatca gttttctcga gaacaaggag agggacgatg
     3661 tttcgatgga cggctaggcg cacggtaact ttcatattaa gaatgttggg tgtgagcgga
     3721 tgtgtgttgg cgttaaccct tcctgcttgg ggacaaggta tgttgatcat tgaaacgccg
     3781 ggagcgcgcc ttcctcgccc tatcatctgg ccaccttacc ctcctcctca tccgccgatc
     3841 ccgattcctc cgccgcggcc tgttccgcca cccccggaga tcagttactc cgtggaatcc
     3901 ctctccgtga atggacgcat caataaccag gtagctcagg ttgaggtggc gcaaacattc
     3961 cgtaacaccg gtagccggac cctcgaggcc tccttcgtat tcccgttgcc ctacgacggg
     4021 gctattgatc ggatgaccct catggtcgat gggcgggaaa tccctgcgcg actcctcaaa
     4081 gccgacgagg cccgccgaga atatgaatcc atcgttcgtc gcaaccgcga tccagctctt
     4141 ctggaatggg tgggcaccgg aatgtttaag acgagtgtct tccctattcc gccgggccag
     4201 agtaggactg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcgccaaa
     4321 ctcctcagtt ataagtcgcc ggatgaggag gacggctttt tccttctttt ggctgcccct
     4381 caaatcgcta cgcaagacgg caagccgttg cccaaaacgg tgatgttcgt cctcgaccgt
     4441 tccggcagta tgagcggcaa gaagatcgaa cagctccggc aggccatgca atttgtgctc
     4501 aacaatctcc gcgaggaaga ccttttcaac atcatcgctt atgacagcga tgtggaggcc
     4561 ttccgtcccg agttgcagcg gtttaatgat aagacccgac gggaggccct ggcgtttgtc
     4621 gaaggattgg tggcaggagg tagtacaaat atcgacgccg ccctacggac cgccttttcg
     4681 cagttgcaag atgacaaacg gccaacctat atcgtcttta tgacagatgg tcttcccacg
     4741 acaggcgtca ccaatgaaat gcagattgtg gaaaatgcca agaatgcgaa caccgtcaag
     4801 gcacggatct tttgtttcgg tatcggttat gatgtcaata gccgattgct ggacaaactg
     4861 gccaggactg gccgcggtta tacggaatac gttcggcccg atgagaacct ggagacgcgg
     4921 atcagctcct tctatcgccg cattgaggca cccgtcctga cagatgtcac cgttgatgtg
     4981 gtcttcgatg agccaccggg acctccgccc gtctatcgtt tgaatccgca accgccgttc
     5041 gatttatttg ctggtgagca gcttgccttg gtgggacgct atcgcaagtc gggtcgcgct
     5101 gaggtcgttc tcaagggccg gattgacgac gagcaaaagg agttccgttt tcccgtcgat
     5161 tttgtcgccc ggagcggaga tgagtcgaag gccttcgtgg aaaagctttg ggccattcgg
     5221 cgcgtgggcg atatccttga cgagattgac ttacgcggga agaacccgga gttgattgac
     5281 gaactgaccc gattggcaat gaagcatggt attgtgactc cttacacatc cttcattgcc
     5341 aacgagaatg tgccaattca cgatttgact cagtcgcgac gtgaaacgga atcacgactc
     5401 agggccttag aagccgccga gggatatggc ggtttcgccc agcggtcatt caaggggcga
     5461 atgcaacaag cccaagcagc ggatgcggcc tcggcgcttc gctcattcgg cggcctcggg
     5521 gctgagttcg ccgccccctc cgccgccgca cctggtggcg ccggtatgat gggtggtgtg
     5581 gcggccagtg cccccgcgtt ggcgcccgct gaggaggaaa ttgctcgtgc ggccacaggg
     5641 gaaacgatgc ggtatgttgg taaccgtgtc ttttacaaac gccaagggca atggattgat
     5701 ggcacactaa agccagaaca gcagcagaac cccatccgca tcaagcaatt cagcgaggag
     5761 tatttcaacc tggtgcgtca gtacgggcgg caagtctcac aataccttgt gttcgacgaa
     5821 ccgacgctcg tcaatatcaa caaccaggct tacttgattg aaccttaaca taacaggtca
     5881 ggagcatcgg tcacgttcct tggggacaac gttgctcggg ccagactagt tgtggggacg
     5941 tgcttcggcg cgtccctttt tatgcgcagc cggtgtcgtg attgtcgata aagaaaatgc
     6001 gaaaacgtga aactgtcttc tcggtcctct tggcaacggc tttcttcttg cgtacatttg
     6061 cggctgattt ggaacttatc gcaggcatca tgagaaaaag gggtatggtg tgaattgatg
     6121 aggtgacaac tatgtcggga cgcatcccgc tcagggcatt aagtgaatta gttagcgatg
     6181 aggaaaacga tacctttgcc ctgctcagta gcaaagagga actgaccacc cgcgatggca
     6241 agccgtactg gatggtcacc ttccgtgacg cccaggtgga gtttactttc ccgatctggt
     6301 gcgattctcc ctgggctgcg gcgtgtcgcg agcaatgggc agtggggacg ttttataaac
     6361 tacgggttgt tcgtcgcgag acgagatacg gtcctcaact cgaaatccat cgcatccgag
     6421 aaaccgtaga tcaagaccga gcggacggtt ttgatcccct gatgtgccta cctcaatcgc
     6481 ggtttcccgc tgaagaactc tttcaacgtc tcgaggcact tgttcaaaaa gaaatcaaat
     6541 cgccgtccgt gctccagctc ctcgtgatga tcctccatac gtttcgcgag tcgattctta
     6601 ctctcccggc cgcacggagt caccatcatg cctatgttgg gggttggttg gaacacacct
     6661 tgtccgtcac cgagacatgt gtgtttctcg ccgataaata catccaactt tatcccgaca
     6721 tgaaaccgcc tcttgaccgc gaccttgtca ttgcgggtgc cgttctccac gacatcggga
     6781 aactgcgaga gttggagttt cagatcacca caacagagta ttcgccagag ggggcgttgc
     6841 tcggacatat cgtccttgga cgggattttg tccgtgactt cgcgcccaag gcgggccttg
     6901 attcagagca gcaacttcgt ctggaacact tgatcatcgc ccatcaaagg ctagcggaat
     6961 ggggttctcc acggccgccc atgacgccgg aggccctcat cgtccacttc gccgatgacc
     7021 tcgacgcgaa attccacatg ttttggaagg cacttcagca cgactcaaaa gaaggtcctc
     7081 tgaccagcga tcgcaatccc ctgcggcagg ccatttttcg gggtttaaag taactctgag
     7141 ccccatatcc ttggcggcgg gttctattct acttatatcc gctgggcttg gatcacgcgg
     7201 ggcagacgcg ccagatcacg gacaacaggc cccaaccgga atcgcccgtc ggcttccacc
     7261 aattcgcata cccgctggca gatcatagga gaaatctcaa agatcaaata gccccctgtt
     7321 accagctttt ccgctgcttg aggaatcagc cgctcaataa cctccgtacc tcgcggtccg
     7381 gcaaccaagg cttgataagg ttcatagttg cgcacggtgg tcggcagatt cgcaaattct
     7441 gcctcgctga catagggcgg gttggaaatg atgaaatcga aagtcatctc agacggaacc
     7501 gcctcgagga gatcgctttc cagccattcg attcgatcgg ccacgtgatg acggatcgca
     7561 ttttccttgg ccactgccag ggcctgggga ctcttgtcga tggccaggat ttgactgtcc
     7621 ggcaaatgct tggccgcaca aatggcaagg accccggagc cagttcccac atcgcaaatc
     7681 cgccacccgc cctggccaga ttgcctcgct cgctcaagca aggtaacgag gacaaactcg
     7741 gtctccggtc gcggaatgag aacatcgggt gtgacccgaa acttcaggga gaaaaactcc
     7801 ctctccccaa ccagataagc caccggcacg ccttcggctc ggcgacgaat catctcacga
     7861 aagcgactac ggacctcctc gccggggaca tcttcgtagt gggtgtacag ttgaatccgc
     7921 tcccaaccat gagcgtgggc aagaaggact tcggcgtcca gtcgcggggt ttctgaaccg
     7981 cgctgctgaa gaaactcggt cgtccagcgg aggagccggc caatggtcca aacctcagct
     8041 tgcgtcatgc tcggcgtctt tcatcttcca gcacacgctt ggccaccaca gaacctgcgt
     8101 tagagcgtat cccgtaatct cccttacccg ctcgggggaa gaggggtcgg gagtgacggt
     8161 ggctcgaatc gctgccggtt ttctcaatcg attctgacgt tcgcttgccc tcaccccaac
     8221 cctctcccga agggagaggg gggctttttg ggatagggtt ttattgtgcg gcgtcggtac
     8281 aaagagggca agaatcgccc cctattggta gcttatcaat ccagggaacc gaaggtcaga
     8341 cggagctcat tacgctcgta atcgagcagg gcttcgatga gtggatcaag gtttccctcc
     8401 atgatctgat ccaactggta aagcgtcaga ttgatccggt ggtccgtcac gcgattctcc
     8461 ggaaaattgt atgtgcgaat ccgttcactg cggtcccccg agccgatctt gctccgccgt
     8521 tcttgggccc gctcggcctc tttggcccgt cggtagtatt cataaactcg ggtcttgagc
     8581 acccgcaggg ccttagcaaa atttttgtgc tgactttttt catcctgaca ctgaacaacg
     8641 atgcccgtcg cataatgcgt gaggcgaatt gccgaggagg tcttgttcac gtgctgcccg
     8701 ccgggtccgc tggcgtgata cgtgtctcgg cgatagtctt ccggcttgat gtcgatctcg
     8761 atgtcttccg gctccggcag gacggctacg gtggccagcg aggtgtgaat acggcccttg
     8821 gcctcggttt ccggaacccg ttgcacccga tgcccgccgc tctcgtactg gagcctgcga
     8881 aaaacctctt ctccttccag accaaaacta atttccttga agccaccaag ttcggtagga
     8941 ttcatgccga ggatttcgag cttccacccc ttggactccg catatcgccg gtacatctcg
     9001 aagaggtcac gggcaaataa ggccgcctcc tcaccccccg ttccggctcg aatctccatg
     9061 atgcaccggg tacgattggc atcttccccg cccaaggcca actcgagtaa ctcatcccaa
     9121 agggcttccc gttcctgccg gaggcgtggc aactcctctt cggctaaatc ccgcaattca
     9181 ggatcttccc cttcgatcca ttggcgaagc tcaagaatct gctgattgat ctccttgaac
     9241 ttgcgatact tgacggctac cttggccaac gaaccgtgtt cccgcatgat ggtggccatc
     9301 cggcttggat cagacaagac cgccggatca tggaggagcc gctccaattc ctcaaatcgc
     9361 gcaagtttct cgtcaagctc ttttcgcata caagtagaac gtcagcagaa tgggacgata
     9421 cgtcccaccc atcggtccaa tgagcacaag agacccctct tcgcaaccaa gcacgaaccc
     9481 tatcaccggc cgcaactctc gacccacgcg gtcaaaaacg atggtccctt cccgctcatc
     9541 atttcttctt accaacgctc gaatagcctg ccgcaaattt cgacttaaat ttctcgatgc
     9601 gacctgtcgt atcgacgtac ttcagcttgc cggtgaaaaa ggggtgacac acgttgcaaa
     9661 tgtcgatctt caactccggc ttgatgctac gggtcacaaa actgttaccg caaccgcatg
     9721 tcaccttgca ctcaacatat ttggggtgaa ttccctcttt cattcaactc aacctcctca
     9781 ggtgtgtcat tattactaca gttgggagga cgtttcccaa cgaacgtttc gcgatggtac
     9841 cctcgccagt atctgcaagg cggggctggc ccactcatct tactcggccg cattctctta
     9901 cgagacgaca gccccctaga caaggccgcc cggcgacgtc ccaaaaactt gattttacct
     9961 ttttgccaga aaggccacaa taagccactg ctgccgaaac ggggcttcac ccagcgaatt
    10021 tggcaccata aagccccggc cttgcgggcg atggtcatct cttccgaaag cctctagggc
    10081 cgaaccgccc aacctcaaac aggcatactg cctgcgattg gggttaaact cccacctcga
    10141 cctattcttg cttcccggac gtcttgtccg gttgtgacgc cttatccgcc ttgttggtgg
    10201 tgggctctgg gctgatttcg agaggctttg cgccggtaag ctcttcgatc agtcgagcac
    10261 gcacgagatt tccaaaccgg ttcgggagtt gaaattcact ccggtaggtc gtgattgcct
    10321 gcgagtagtc ccccagcttc tcattgagtc gccccaaggc ataaaggacc cccgagtgcc
    10381 agggctgatc cggtaagacc attgcagtcc gattgatcag gtaatcttcc gcactttttc
    10441 gatttccctg atccacggca atcaacccga gccaatacga ggcattcagt ttcgcgatcg
    10501 tgtacatacg ctgctcgtcc gctgcaagct gcccactgtc aatcaggcgt tccggaggac
    10561 gggccaactg atagtaatgg gccgcccctt cttcccccac gagattcccg cgcaaatgga
    10621 ggattcttcc cctccacagt ggcatgatcc ctgtttcgga tgtcagcatg aagggcgcta
    10681 tttggttaaa aaggtacttg gcaacgacgc cctcaaattc ctttctctgc ttttcaacca
    10741 agtacggaag tgcccagagc tggacgtcac gaacatgcgc caggccccga aatcgcatcg
    10801 ctaaatcgct gggatgcgcg ctgatcacca aggactcctc gccagaaagc atgcgttgaa
    10861 tcatctccat gcgtcgcgac aggtaaggtg ccgatccttc cacatacacc attacttgct
    10921 caagttggga tgcccgaacg gggtacggat tgcgcggatc ggcgtccatc ctgcgcaaga
    10981 ggctgtcatc taccgccaat tcgtccaccg tcgccggggt gatgaccagc ccctctccgt
    11041 ccacgacctg gacgccgtct cgggcaggga taggcatccc aagtgccggc tcgaaaacat
    11101 agattttgcc gttgatgagc actcccacgg cccacatgtc ccaacggggc tccgccccag
    11161 ccggtgcccg attgacactc aaaaccgcag cgtctaggcc ctcctgacgg cacagcaaga
    11221 cgtaaaccca cgcccgatca attgctgaac catgccccaa aagcaaggtc tcccagggtt
    11281 tttgaacgag cagatcaccg gatggcagtt ggcgagcttg gtcctgggaa tcgagctgga
    11341 tgtttaaaac cacccaatcg aacaaacggc atgccctggc cagctcgctt ggatcctcac
    11401 cccgtgccca ggcggcaatg tcccttaacc aaacggccga ttgcagaaag agcccgtcct
    11461 cccggtctaa acttagcttc tccaaatctt gcaacagttc ggcattaaca aacccctccg
    11521 gaagcgtctt gatcagcgga tcgacttccc actctttgag cgggggttgg gtcttgatcc
    11581 attgatcgag ccgatcagct acttggaggg ccatgtctgt gccgtcaaat tgatgaagac
    11641 ggttcagatt ggctatcgca atttcaaata gctcgtcccg gtggagcgtt gccgtctgtt
    11701 ccgcgctggt agtgtcgctg gtcgtttctg gttgtcgcgg cctcgggcac cccgcaaggc
    11761 acaagcccag aaaaacgaac agcaaaaata tccatcctgc cttcctgcga gagaaaacct
    11821 ccgaacaccc ggctcctagc cgtcgcctta gggctggata cgaaatgccg cgaaacttct
    11881 ttgtgatcat attcgttctt ttaaccattc acgcagttcg gacaatgtct cgaccaatcg
    11941 aggcaggtcg gcgagaggta ccatattcgg accgtcgctc aaggcctcat caggccgggg
    12001 atgggtttcc agaaaaactc cgtccgctcc ggctgccacg gctgctcgcg ccagcggtgc
    12061 gaccatccgc cgatctcctc ccgatcgtcc ctgctgacca ccgggttcct gtacgctatg
    12121 agtggcatca aagatcacgg gaacccccag gtcgtgcagg atggggatac tccgcagatc
    12181 gttgacaagg cggccgtatc caaaaaacgt tccccgctct cccacaagga tgttttggca
    12241 acccgcccct tccaatttct caatcacgta tcgcatatcc cacggtgcta aaaactgggc
    12301 ctttttcacg tgaaccggtt ttcctgtctg cgccgccgcg aggagaagat cggtttgccg
    12361 acagaggaaa gcgggaattt gaatgaggtc gcagacctca ctcaccggct gtgcctgagc
    12421 cggctcgtga atgtccgttg tgacgggcag gccgctttct tcccgaaccc tggccaaaat
    12481 cgccaatccc tgggaaagcc ccggtccgcg aaaggaatgg aggctcgtgc ggttagcctt
    12541 atcaaacgag gccttaaaaa taatgggaat ccctcgcgac ttggaaaact cggccaggaa
    12601 aaacgcaacc tcccgggtta acggttcact ctcaatgaca cacgggccgg cgatcaagag
    12661 gagcgattcc cccggcccac accggtaatt tcctacactg acgattcgat taggcacaca
    12721 ccaaactccc gtttttcacc cgcgaaagag attctgactg ccgccgttgt ttttgtaaca
    12781 tttccgccgt tctttcgggc accacaattc gtacccggcc cggcgccagc ccaatatcga
    12841 gcggcaagta gcctgccgga tcgccgtcaa tctggtaagg cacaggttcg ttcgaggtga
    12901 cgcgaaatct tgtcccccaa atgcgaacaa agtcacagac ccgttcatga atccccacct
    12961 gcgtggtcac ggtgtaccac agtccgcgcc aaaatcctcc gcccccatag acgcaaaggt
    13021 ccagaagccc gtccatgccg ttggcttgag gggcaatggg gagcccccac ccgtacttcg
    13081 gcaaattgaa cgcaaacacc cagcggccct taatggttct ctgagacgag gaaggctccc
    13141 cgctcgaacc accttcatag ccatcgctca cttccacggt catctcggga aatggatagg
    13201 tattaattaa gcgacagatg gggatgataa agccggagaa tcctccccgg ccttgacggt
    13261 gtcctgccac ccgcttccgg tgcgccattt ccacggccgc ggcatcaaaa ccgcaactga
    13321 ccatggcgag gaagattcgt ccgttggcga gcccagcatc caaacacacc gtggctcccg
    13381 tttcaattaa tgacacgaca aacgggatgc gttttttcat accaagatag ctggccacta
    13441 agttcgacgt ccccgttggg aaaaaacaga cggggacgcc cggttgaact cgattggcca
    13501 actcgcttgc cgtaccatcc ccgccggcac ccaccaggca gcgcaagttg cctttttcaa
    13561 actctcgatt ggccaaatcg gtcgcttggg aaagattcgt gctcacgtgg agggaaaaac
    13621 ccttttctcg gagggcattc accaattgct caatcctccg atcggacggc ctccgccctg
    13681 cgaccgggtt atgcaagaca acgacaagcc tcgagtcaac cgggagtcta tcctgttcca
    13741 ttaagcatcg cctgaacgaa cgagggacca gctttgccca aaacgaatca ttacaaccac
    13801 aaatgctata ataccccttt ttccgcctcc tgccaccgta cgacggagct agcttaccga
    13861 accatctccg caaacccgat caacccactg aggtgggacg attagtgtgt tccggcacat
    13921 actctttagt gtagcaaaat tgatcaatcg gatgtccggg tgcggcctgc aaaagccact
    13981 tttcgtagcc gattgctgca agtgttgcaa aataatacac caccgccggt ttcgcgacct
    14041 ggccacaatc ttacggctaa tccataaatc ctttttaata atacacttat atcgaacaac
    14101 cgtgtttccg tgataaccgg aacaggagtt gctcgagaat ctagtgacaa ctcaggctgg
    14161 cccgtggggt ttccggcgag agggtgtcat gcgggtacct tgccctaccg catgacgtgg
    14221 ccactgtggg ggtggcacgc cggaagccca ctttttttat cagtccagtg attttctcct
    14281 cctccgtgtg tttccaccga agaaagcctc atctggcgcc ccctcaacaa tgagcgccac
    14341 aaagggcgtc ccgaagttgt gggcctcccc gcctgacgac gctaacggtc taaccccgca
    14401 ataacctgtc gggagatttc ttcgaaagga aatgcggtgt agcgtcccaa ttggcgacgt
    14461 tcgtagcgga gcttcactgc cgtcgcttga tcggccgccc gactcaactc ctggtcgaac
    14521 cggtccaaca gctctttgat ctgcgttcgc ccgtcggtat aggcttgggc gagcgattcc
    14581 cccaactgtt tgacaccatt gtccaaattc tgaaatacga cgctactcgg tcgttccgcg
    14641 tcctcagaaa cccgttctgc aaattctcgc agctgtccga taaagtgaaa cactttgctg
    14701 ccgtccagtt gggtaacgat ctgaccagga tcagttttca cctgagaggc ttcccaattt
    14761 tggacaaagg cattgagttc attaacacta ccgttgtaat cccgcctcac cagtttcacc
    14821 catgccagcc ccaggtaagc gaccaattct cgcggccgtt ccagtttcag tgtctcgagc
    14881 acccgttggg catctgcggt ttgacccatc tttatctgga tcagcgcgta tagaacccgt
    14941 ggctccggtt ctctgcccgc tagcttcaac gcggtgcgat aaatgtcgtt aacaactcgg
    15001 gagtcgaggt tttcctgaag caccatggct gccagttgtc gaatagcagt cttggcctcc
    15061 tcagagagcc tcggtggtcg attatcaggg aggaaaattc gaggcctttg cttatcttgc
    15121 gccaattggg gcacctgccg tgataaataa ccataaagtt ctcccgcctc gatccgtcca
    15181 tctcgattct cgtcggcctt tccttgatat gcctcgatca gggctgcgcc gaaaacagtc
    15241 ccgtcccctg ctagcatcgc ctcttggcct tcggcacacg cagcaatggc agtcactgtg
    15301 cgaaacgggg ccattcccgg cggtccctgc accgctttaa gggcctcctc cgccgagaca
    15361 aacgcctgtc ccgatgcctt ggctttctcc ggagaaacat ccactagcag gatcttgctt
    15421 ttcgcgctcg cttgttcaag gctatcggca agaaattgga cggacaaagc gctcgtggca
    15481 agttgttgca gatggctttc tttgcacaaa agaaaaacgc ggccctcttg gtcttttgcc
    15541 gcgtaaccgg cgtaataaac gacgacatcc gcattacccg ggacgttttt caaaaacgaa
    15601 ctgaccgcct gttccacgcg aacgcggctc gggttcgtca gaacaagcgt ttgattccgc
    15661 ggaaacgccc cccgctgaat gagcacgtcg gccaaacgtc ttacaccctc cacaatacca
    15721 cgagcggccg ggatgttttg atcgtcaaac tcttccacgc caatcagcag cgcaaagcgt
    15781 gtttggtcag caggtcggat cgcggcaacc tttagtgccc gccctgtggt tgtgccgggc
    15841 gtatcgtgct gaatatccac ctgatcccct ggccgtaggt cgacaatcgt ggccggcgac
    15901 cccgcgattg taatggtaca tgtgtccggc accaaaaact gcgccacctt gccatcgccc
    15961 gtcaactgaa catcaatggc gtttgcgtcg aaatggactt gctggataac ccccgccacc
    16021 cccaacacac ggtaggcgtc aatgcgccga atctgatcat cgtgctcgat ttccacctga
    16081 tcattaggca gaaggtctgt cggcttttga agttgcccac caatggccgt aaggccgttg
    16141 actgtgacga cacaatcagg ggcccagggt aatgtaagcg tcttaccaga atcgctcaag
    16201 gcaagtcgga tcgattgttt ggaagcatca acgacctcca cgacaccctt ggatcgcatc
    16261 acccgatatg cgtcaacctt taaggcaatt aactcgtttt ccgaccgttg atattccagt
    16321 tcaacccggt cccccacggc gagatccgca aggcccaatg ttttgccgtc aatcgtctcg
    16381 ttgccgttca agcggattgc agcggtgttg ggaactcgca cgacgagctt aggctcccct
    16441 ggactacccg taacgcttag tgttcccatt tccggggaca tctcttcaat gcgtccggca
    16501 aatcgttcag gacgcaaagc ttcaatccgc cgaatttgtc gcccctgatc gtcgcgcagg
    16561 acggttatct tgatctggtc acccggttgg aggtcacgca gtaacgactt tttgtcattg
    16621 acaaagactt cgtcccaagc gcgaatctct atttcctctt tgcctagggc tcctcggtca
    16681 acaatgagca atctggcgtc cagcaggaca ttctccacac gaccggaaag gggctccacg
    16741 ctgacggctg ggggtgggga ttgcttttgg ctgggccaca gcatcaatgc cgagatgacg
    16801 agagaacaag cgaccgcggc aatggcaatc atccgtgtcg cacgtttcct tttccgctca
    16861 gccaattcct cttcggaggc ctcttcagaa acggggggtg gcggtacttc ggctgctggc
    16921 cgcttcagtc gctgaagcgc tactgccgcc gaggggattc tgtcttgctg attcttttgc
    16981 acgagacgat cgacgacttc ggctaggtcg tcaggtaccc cttctaagac gcgatggatt
    17041 ggcgggagtt tttggtcagc cgaggcatgc cacatcatcc aggcaatttg cttatcccga
    17101 ccaaaggtac ttaatgtggg aaaaagtaat tcgaagtcgg gaccgcatat caactcgtag
    17161 gccgtgaatc ccaacgaata gaggtcgctg gcaggcccca cgggcccaaa ctgggcactg
    17221 atcagctccg gggccatgta ctttgtagtc cctttgagga ggctgccatg ctcgtccgtg
    17281 gcgcggcgcg ccaagccgaa atccccgagt ttcactcggc cttggttgtc caaaagcaaa
    17341 ttgctcggtt taatgtcacc gtggacaatc ccgtttctgt ggaggaaatc aagggcttcc
    17401 aagctgccaa tgaggatttc tcggagaaag tccagatcaa tcggaccttt ttcaatgctt
    17461 ttttgaagac ttgtccgcat taactccagg atcagccaac cccgcgattt gacgacatca
    17521 taaatcgtaa tgacatgggg atgctgcaag gacgccagga gctgcgcttc acgccaatac
    17581 cgctcgagtt gtttgggatc acgccggaaa tggggatgaa tctccttgat ggcaacgtcc
    17641 cgccccaatt cccgatcccg cccccggtaa accgtggcga aatcacctgt ggcgattgtt
    17701 ccgatgattt catatcgtgc ggcggcgccc atgggcaacc tcgacttgcc tcccgtggtt
    17761 cgaacgttct tgatcgcctc tcgtttctaa tcccgccgag ggagcggcct gacgccgaga
    17821 ttcaatcctt aagaaggatc cgcagcaatt cctcaagttg ctgatcccgg gtgcgactcc
    17881 ctgtcaatcc agtataggta tttcggcccc gttctgcgta gagtagcaaa agtacttggc
    17941 ggtagagttc tttttcccgt ttatagtcac cccgactgtg tgcccaatga gcctctcgca
    18001 agagacgctg atattccgcc aagggggcat tgggattgag gtgagaataa tactctgcgg
    18061 caaggatttg gcgggccctg gctttttctt cgggacttcc ctgcgtggtc ggtgatgtat
    18121 caacaaaaag caggatcaag cttgtcgcta aactgctgca caacaccaaa atgagaagaa
    18181 gaggattact cgatcgtctc ttttccttcc ctcgctcctc gtcgctgcgc tcctcgagat
    18241 gcaagctcgg aagactgccg tccggggcaa gcgtccactt gggttcggcc gtttctgtaa
    18301 ggatgagtcg ggctactctt cgccgtggca tcgtcagtga ggtgtcgccc cccgcaggtg
    18361 attctccagg gctgggtttc cccggcgcca cgccggccgc tcgttctgac gcttgacctg
    18421 ccttatcagc aagaacaccg cgtccctcgt cccgcccggc tgtttttccg gggcttggtt
    18481 gtccaggccc gactgagacc ggtttggaca gcctagcctc ttctttgctc acgggctgcg
    18541 cggccccgtc aggagtcgcc acccggccaa ccggtcccgg tgtttcccca gtcctcgctg
    18601 gcttcttcgc tggctcggca cctttaggtg cagttggggg agtcgccagg ggaacctctc
    18661 cctttggcga ggtcggttta gctccctgag ccaagacagc gtccgctcga aagcgatgac
    18721 ggcaccacgg acagcccacc gttcggtcga gggctgtatc tccgaccgtc atttccgttc
    18781 cgcataaagg acaatggatt ttaatcggca tcgaaaggtt cgaccgatgt gctctgtttg
    18841 atgttaaccc gataggaatt ccttctcagc ttttcatccg gcattgcttg cccaagtcat
    18901 cgttggattc ggctacgatt gccttaacaa aagccagtca accaaatctc ctgagagcct
    18961 atccataatc tcccctcacc cgttcggccg gagaggggtc gggagtgagg gtagctcgaa
    19021 tcgctgccgg ttttctcaat tgattccgac gttcgcttgc cctcacccca accctctccc
    19081 ggagggagag ggcgactttt tgggataggc tctgaatttc gctggccaag tcgtcacacc
    19141 cctggcttac atgccatgaa aataacgctc acaacgacat tccaaaatac agatagttca
    19201 taaggaacgg gcctaccaag atcaacacgg cggcggccat gaccaaaata cccggcagca
    19261 acatattgac gcctgcctcc ccggcaatca tttctgctcg ctgggatcgc ttaatccgta
    19321 acacgtcagc ctgggtgcga aagattgaag ccagaggtgt ccccaatgtt tcaccctgta
    19381 ggatcgaccc aataatactc gtaatttcgt catcggccag gcgctctctc attgccgaga
    19441 aggcctcata gcgcgttttg cccatgcgaa tgtcggccag cacgcgaccg aactcttcgg
    19501 caatggcatg tccgcgatat tcttcgaccg cttgtgccag ggcattgaga aatgtggatc
    19561 ccgcttccat caagagcgtc agcagatcca gaagaaatgg caaacgccgc ttgatggcct
    19621 gaagccgcct ccctgcacga tccgccaggc ggagtcgtaa aaggaatacc gtcaaaccag
    19681 caaacaccac tgcaatcaga atgccacctg cccccactgc acgcacggcc acgtaaacat
    19741 aaatcggcaa aaggaggaag gcgatcaatt ccagcttggc caaatactct tcagccagcc
    19801 aaaaccgggg caaacctgca caattgatgt gccgctggat ctccggtaag gcatcacgaa
    19861 aggccgcccg attgaagcgg gcgagcagtt gaatcaacgg ctggaaaagc cgaaagaacc
    19921 ggtcctgacg gcggagctcg ttgatgcggc tgacatcgta acgccattcc gctccttgct
    19981 gcaaatcctc agtttgaagc acctgaataa accaccgggt caacagaaac gccgccaacc
    20041 cccacatgaa gcaggccact aacaacagcg actggctatg ttgaaactct ccaaattcca
    20101 taatgcatca ccgataggat gattgctcgt aaatggattt gtcgatcact tcccaagtcc
    20161 gcttggatta actacctcct ctttctcaca tcaaatgtcc gggcgcaaca aggcgcgggc
    20221 ccacaggtag gcaaccacat ccaaaatgat ggccattcct agcagcaaat gtcccaacgg
    20281 ttgcgtgaac aagagcattg tgttttccgg gtccatgaaa taaaaaccga tgaggaagac
    20341 caccggggca aaggccatgt agatggccga cttgcgggcc tgggccgtct ccgaaagcac
    20401 cttccgttcc agccgctgca attcgaggac cgaaccggcg atccgctcaa cagtctcgtt
    20461 tagtcggcca ccatgttcat gactcgccaa aagggctgcc gcaaaaaggg caaaattctc
    20521 acttttaagc cgctccttcg cctcgatcag cgtccggtcg agcggctttc ctaggttgta
    20581 ctctgccacg atctgtcgga actcagcatt aatcggccga gggcactggg ccgccaggat
    20641 ttccaaggct tgggcaagcg aaaggcccgc gcgcacggcg ttggcaaggg tcaccatggc
    20701 gtcggccagt tggtcttcga ttttccgatg gtataactct gccaagcgac gtaacagata
    20761 ccacggcaca gccagaagga aaaccgccgc aatcaaggcg aagatcaaac tacccacaac
    20821 gaatgtcaaa ccgacgaatg ccgcaacaac tccgcctagc cacgccaaga gccaaaatcg
    20881 aagatgccgc gtgtgcaccc gcaatcggcg aagcttttcg gcaatgtcag cctccatttt
    20941 caagaaggcc tgggcaatcg tctctcggcc accgtacacg attagtgcga tggccaagcc
    21001 tgttgccaag gatcccagaa aaagaaggtc ccgattatcg ctcatctcag attcgcaacc
    21061 gtttctcttc taacgggcca cttcctgccg gaccaccaac ggcctctgcc atcgagaaca
    21121 tttccttttg gtgatcgtcc tttccataca gaaaatccaa atcgagcaag tctttctcaa
    21181 ccaacgagtc gataaacgtc ggcaaatagc ccgtcggctc aagtgagaca ccgtttctaa
    21241 agttgaagat gtcgtggagg atcacatttt tcgtttcggg atcgacgcca acaacttctg
    21301 taatctctgt gataacgcgg cgaccacccg gcaaacgtgt gatgtggaca atcagatcga
    21361 tcgccgatgc gatttgacga tggatagaat aaatcggcaa ctccgctgcc atgaggatca
    21421 agacttctag ccgttcgatc acctctcgtg ccgtattggc atgcaaggtg gtcagagagc
    21481 catcgtgacc agtgttcatc gcctggatca tgtcgagggc ctccggccca cggcattcgc
    21541 ccacgatgat tcggtcaggc ctcatgcgca aggcattccg taccagatcc cgaattgtat
    21601 aagccccctg tccctccaca ttcggcggtt ttgcttccag ggtcaccaca tgttcctgat
    21661 gcaatcgcag ctcgaccgta tcttcaatgg tgacgatacg ctctttgaag gggatgaaac
    21721 tactcaagac gttgagcagt gtcgtcttac cggcaccggt acccccactg acaaggatat
    21781 tctttcgatc gatgacggat gcccgcaaga acgtcgcagc cgccttgcta atcgagccca
    21841 tctcaatcag gtcatccatt gtcatccgct gaacgggaaa cttgcggatc gtcaggcatg
    21901 ggcccttcac ggcaagaggc ggaatgatgg cgttcacacg ggatccatcg ggcaaccggg
    21961 catccacaag gggctgactc ttgtcgattc gtcggccaac ctgggccaca atccgttcga
    22021 tgattgcctc ggtcacctta tccgagataa agcgccgccc cgatttctcg attacaccgt
    22081 tccgttctac gtagatctgg tctttcccca ccaccatgat ctctgtgatc gagtccgccc
    22141 ttaatagatc ctgaagcgga ccaaaaccaa agatggtatc tttaagatct ttcttcaagg
    22201 ttcgaaggat taggtacttt cgtaactccg catggagatg tggtcgaacg agttggaaga
    22261 caacgttgaa atgttcctca attcgttgaa cctgctctgt ggtatcgcga cactcggaga
    22321 gccgcagtcg ctcccgaatg aaattaagaa tacccgccag ttccgtctct cgttctggga
    22381 cgagtgtcgc cgggatgtcg aactcgttgg catttaagcc cgtcatctca aagaggctat
    22441 ccttgcccga ttccataatg aggctattga caaggtaatc acggagcatc aatcccgtga
    22501 tctcttccaa gatcggctcg ttttctggct caaagactcg caactcgcgg caaatatcct
    22561 cgatattgtt ttccaaaagc aagatgctct gcgggtcgtt ggtgggccga ttctcaaact
    22621 cgtacaggtc cagtcgctcc agcaacttcc gatggatgcg caactcgagg tcagccacca
    22681 gggagcgaag atgggcctcc aattcttgcc gcgagatgtt gacggccttc tcggcctcaa
    22741 atgtcaaggt gtaaggccaa atccgcactc ttgtgcccgg tgaaaaaaca accgtctgcc
    22801 cgcccaaaac ctcctcatca cccaccaggc aactgttaag ccctaagttc tccagttgta
    22861 aacgcccgtc ttccagacaa cgcacaatgg catggcgacg cgagacgagt gggctggcca
    22921 aaacgacggt gttcgaggca tcccggccaa tggtaacctc ggccgcgtcc acctcgatca
    22981 tcgtgcggtg actttcgttg atccggttaa accagatttt catgagctgc aacccattct
    23041 ctgttgcccg cgaaacggtg cgtcctgcat acccgttcga tcattcgatc acgtcaattg
    23101 ccagaggctt cccgcgagtg taggataaca cccacctgtc ggaagtttgt cagctccaac
    23161 atcttccaaa ggcgttcggc cttaggctcc aggcgtccac cttccaaacg aacgctgatg
    23221 gtcaggatgt tctcctgtcc ctggggcaac tggtaagccc ggtgaacctc cacgctgctc
    23281 aagcggtttc cgatggccaa gaccttgaac ttaccgatca cttggggacc accggacaac
    23341 gaactgcccg cggcggaacc ctccccgccg gcggccggat cgggattacc cacacccgca
    23401 gtgggggtcg ggccacccac aataaacgac accatgtcac ccggttccaa aagcgacgga
    23461 acgatcgttc gcacatcaat cgggataaac atggcgattt cctccgtgtt ccccaccggt
    23521 tggcccccga accgcaactt cggcggcggt ggacgcaggt catcctgcag gaggagcgaa
    23581 ccaccaggga tgggacgtgt aatccgcaat cccacaatac tcgcccgatc ggcgtataaa
    23641 tagccaaaat ctttcaagcg tcgtgccggt atggccggga tcggcacggg cacaaggtct
    23701 tcctctgcca agatgtgacc ggcctcgagg tctttgccgg gggcaatccc gacgaaggca
    23761 accgcgtcaa tatcacccgt tgcccgagtg atgtaagccc aattgaaaag tgccgcagca
    23821 atccccacgg ccacgcccaa gacgactccg tgaatccctt tcatcgccta tcgctctcac
    23881 acttcgcgag tctcggagga catttgctcc ctacgtcaat gactaaaccc ggtttacgaa
    23941 ctacgcgtag ccctcctttt ccattctaac cgcaaaccac cagccctgtc ccctgttcca
    24001 atcacaagcc atttactgtg gttccgaact tctctcccgc ttctccgtgg tccaacggaa
    24061 cccgaacacc aaaccccgtg ccggtcggtg ggcgtcttga gttatttcga tgctgagaaa
    24121 actctgttcg gtcgtttgag tgcctcggcc aaaaacccgg cgtttcctcg ccgccccccc
    24181 caaccaatcg ccgaccggca cccaaccttg ccgctccagt tctcgatcca aaaaaagcgg
    24241 ccaggtcaca gaggcccccg gcgtggactc ttcgaaggcc ccgaaaaatg cgccctcgcc
    24301 tggagacaac ctcgaaacaa cagtcgcccc cggcgggaca ggaaacaaaa actcttcttc
    24361 accaagcgac ccaagtgttc ctttttgtgg cccttccgct gccggtgaaa gcagatagat
    24421 cgtccactcc ctctccgtga ccggcattaa aaaggcccag gccacaatgc ggctcccagg
    24481 gggaagtgaa cccgcgtcgg ttgcgtcggt gtccactgca ccagcccgat ctgtaccttc
    24541 actttttttc tccccaagtt tgactccgac ccaaagggga acgtctcccg gatatcgata
    24601 cacccgactg ccattgggtt cctccctgat cgggaggata ctagccaact ttgccaagac
    24661 acgcctttcc gccgcgtcag gcagcccggg gcgcaccacc gcacgctgga ccgcctcaag
    24721 acatgtttct tcacaacggc ggatcgcggc cttttcttcg ccctgaaatg agatgcaaga
    24781 aaaaccgcca ccgccaccgt aaaactccgc ccatcttctg gagacctgca ggctttctct
    24841 ggtgtctctg ttaacagtgg tctgatcttc cgtggccgtg tctttccacc acaagagcac
    24901 ctgcacgcca aacaccaggc ccgcggctag aatcaaaaat gttacgagca aattggaggt
    24961 ccaaccggtg atccgcttgg ccaatttgct ccaccccagc tccgaatcaa ccgccaacgc
    25021 cctactcggg gaaagattca cgccatcggt ttggggagaa tctttccgct ccaattcggt
    25081 aggtatctca gggtgagacg gatgatcacc ggccatgatt ctgcaagctt tgccgaaacg
    25141 cttcgagagt gtcaattttt tgttggagat cggcaatttc ggcctggatc gccgcaatct
    25201 gactggccga tggtggtggg acggcgttca attggttttg caactcctga atcacacgcc
    25261 gatacaaact gatatacgct ccggccatcc gttccgccag cgatggtacg tggttcactt
    25321 gcggcgggcc ttgtttcggc gacacgccgg cgattcggtc gatcaagttc tccacgcgct
    25381 cttcggctac actcaagtcc aatgagcaaa agtgactgag tccgggatgg aagtccggtg
    25441 atcccccacc ccgccagcca aagcgggctg cataggccgg gaattccggg tcacgatcga
    25501 gaatcagaag atcattccgt tggggcatcc tcaggattgc caaggccgcc tgcacgtata
    25561 acgccaagta attcccaccg gccgtgggca atcgatacag ggccgtcacg cggtgcgccc
    25621 aatgatcgtg ccaccaatac ggcaagcccg tctgccgata tcgccaacaa ttatccagga
    25681 ttggagctct tgaactcagt tgataacccc caagactcgc cagcatcggg aaagatcgtt
    25741 caatctcgga cgtgccccgc cgaaagccct tcgtaggatc aagcaaatga tcgttgacaa
    25801 caaaagtgcc gagggtcggt ccgcgagcca caggcagatt gacgcgcggg tcatcgagca
    25861 caggtgcgtc gtaatctgct cccgctccca aattcgcagg aataggccag taatcaggcc
    25921 gggggaccgt tcccccattg cgcggagggc gatccgccca caccgcttgc cgggccacgg
    25981 ccaggccgcg gacccgccag gcagaaaccg tcccaaaatt gatcatcaac gccatgatga
    26041 acaagaacaa cggcacagcg atgacgaatt cgaggggggc caggccgcgt ttgccgaaga
    26101 ccgtgccgcc acgggggaga ctttggctgc aattgacctc cgcagcccat cgcagcgggc
    26161 taaatttggt catcgttcct ttgtcggttc gaaccataca cgcacgctgt tgcaatacct
    26221 cagtgaggac tcacgtactg gatatccgcc gtcgtccacc cgggaaggct gggaagctgc
    26281 acattccatc cggcgaattc cggaagaggc ggctttgttt ggacaatgag cggcaaactc
    26341 cacacagttg ccggttgcag ggcacacgtc caattctggt tgagcaaatc ccagtgcgtg
    26401 gggacatcct ctcgaaccac cctccactcg ccctgagcag gttcggattg ctccgcgtca
    26461 tctggtggaa gctcagcaaa atcgccagga atcccgccca aaatattctc aggtgtgtcc
    26521 acgtaataat gccatatcag ccgggctcgt ggaatgaaca cccgcacttg agcgtaagcg
    26581 atcgtatccg atcccatggg atgctgaaac aatcccggca acgttgccct catcggacgc
    26641 cagtaggcca cgccaacaaa cgtgaaatac tgatcgagat gggaattcgg atcgacgacc
    26701 tcatctcccg gtgtttgaat cacaaacggc aaattgcttg ccgggtattc gctcaacagt
    26761 tgttccaatt gcccacacgt aaagctccgc cacaacgagg cgaattgact catcttcgcc
    26821 tcgcggtcga aaaaaatcag cgaacgatta ttccacaacc gcagatagtg atgagacaga
    26881 ttccgtcgct gctccacggc cgtgttgaaa taatcggcct gattgggaaa ctgatccatg
    26941 acgggatcga cgaccggcag cgtcctttgt acaaattcgc ccggcccacc gacgggaaca
    27001 acgtttgtcc gccaaaggac acccaacatc tccccccggc caaaatcggg tctgccgttc
    27061 cgcctggcaa cttccatggc cgcctgctgg gcaatatccg gaaaggccgc caccaccgcc
    27121 cgctgatact cgggaatcag ctcctcttga agaatgaatt ccaacgtagg aagaatcacc
    27181 gggctcgcgg cggccgccca actgcaaaaa ccatccacca aagcctgttc taacggggtc
    27241 tttgcaggga tagccgatgc cagtggaata aacttttcaa aatttgactg agcaaatacg
    27301 ggcgcctctt gattccaggc atctaagatg gccgggacat aactctcagc gtgtcggtct
    27361 cttgcttccc gcatgtaggc cgtaagggca aagacatcac aaaggaggtg attggtgaag
    27421 gccaaggcgt tcatgccgcg ggcaatcacc actcccccgg aataggccac agcatcggcc
    27481 gcattctgga ggcggatctt atggtccacc acccgcccca cattcatgac catcccaagc
    27541 aagatagtga gcaagagtac ggcgaaaacc gaaacgacac tcatcgcccc atcttgatcg
    27601 gcagaaagcg atcgcaaccg acatcccagc ctcgccgcac atttccgcat cacggcgatt
    27661 gttcgcacat caccctttga gagcatattt tttcgccggc agcacgcctt tcctcgatac
    27721 cgtttccgcg cagcctgaga acccacaaca cttacaaatc acacataacg gcagttcagc
    27781 cgaccttcgt caaggaatca acttcctcac cagaaggcga caatcgaata tgtgtagggg
    27841 attaccgatt tttctccttc gaggcccatc gaaatggacg cccgcaacgg ataggtggca
    27901 atcccattcc gacgttcaat ctgctcggca acctcatcgg tccggcgaac tggtcgcgcc
    27961 aggaatcggc ccggcccggg caaaagcgcc atatgatgcg tcagtgtcac cgtcaccgta
    28021 tcctgaaatc ccaattcatt gggccggaat tcgtaaatat cgtcttcgag aaaatatggg
    28081 gcgaggggtg gctcgcgatt cgagtgaaaa aagcggagtt ccaactccgt cgcaagccgg
    28141 gcataggcca gcttattttc caaccgccgc ggtatccgtg gtatgctcga ggccccgggc
    28201 acattggcga gataggcttg ctgcaaaagc tgggcagccg tgccaaggga acccgatacc
    28261 gtgagcccca aatcccgtga gggacaaatg ggcatgaccc ccaaaatggc ggcgtttaca
    28321 attttctgat atttagggct ggctggatca taaggaatga taaacgtcaa accaccactg
    28381 gtaggtccgt attcggatga agttggatct gtgatgggaa tgacctgatc cggggcgttc
    28441 ggatctaaca catagccggg ccctatgcag ttttccaatt cccagtcgcc caccttggct
    28501 gggatccaca caatagccgc ccgcgcggca gcatacgccg cataatggac gacgaccgtg
    28561 ccgatcatga tctggctgac ctggataata aagagcatca atagaacaaa gaagggcagc
    28621 gtaaggacaa agctaaggct ttgcacagcc ccggcttcgt cacggtggag agttaagagc
    28681 cggctgaaat tggcttgggg cttttgcgcc cgcagaagaa gccacgcaac agcgcatgcc
    28741 gccaggagga ctccggccca aggaagacag gcaatcaaag tggctcggag catagaccta
    28801 ccctctctca ccgacagaga gagcttatcc aggccgacta cggccccaca gcaaggacat
    28861 acctctcaac caattcaaat cccacaatca cagtggcagc caaggcactc ggtgccaaaa
    28921 aaagtggagg ttgaagttgc gctcgttcct ccggtgtcaa cgggtcccaa cgtccgagac
    28981 gaatcgacca caaaaggtgg cgtcccgcca gcacgaggag ccgccacggt ccaactcgcc
    29041 acaccagggc aatcagtccc aaacacgccc ccaggacaaa cgtccacaac attgccgtga
    29101 tgccccgatc tggtccgagg aaggtcccca acatggcgat taactttacg tcacctcccc
    29161 cgatgcgaaa aaacacataa caggccagca tgaccagccc gcacgccagg aaacccaaca
    29221 ccgactggcc catgccaatc cagcccaacg cactcaaatg ctcggccctc gcccaaccca
    29281 accgcacagc caatgtgccc aagacattca gcaacaagcc gctgatgatc cctgggtagg
    29341 taatcttgtt cggaatcctc ccccacaaaa catccgtcat cgatgccaca gccaacagga
    29401 tcaagaggat tccgagcacg ggacctcctc aggtcaagcc acatctccag ggcacatacc
    29461 acatgccggc ggcaatcttg cgatgcctac cgtcactgta tggcgttggc ccgttcggct
    29521 tccaattcct gaagtccctg cgtgaagtaa cccttgatcc tcggccaacc tactttgatc
    29581 aggaagatca aaataggcag ggcgatggcc ccgatgatga ggatcgtttc caaactgacg
    29641 gctccccgat catcggcatg aatctgtcgc aacaattgcc acaaactcgc tttcttcata
    29701 ggctgctcct cacacaccct tctctgttgc cggaaactca caaaccgcaa accctacctt
    29761 atcacataaa gggccagcca atcaacacgt acaccatctg ataagtcaac cagataagcc
    29821 gcggacccgc ccacatacaa aacgccgcga cgggaagaat caccccaacc aagaggacgt
    29881 aatccaacgt cgaaacagcc ccccgccgat aacatcgtcg gatgccaaag gctcggctcg
    29941 aaaagatggg cactcgacgt tcggccttca tcgctcccgt cccctggtgg tttgatcacc
    30001 ctccaaccgc cgctcgcggg gtccactttt attatatcga aaatcccccc gaatggcgaa
    30061 gtggaaacca gacattttgc ctttcgaata tgtccatgaa tttgacaccg ctgcgtcgga
    30121 tttggtttcg atttttcatt ggcttggtaa catcttaccg gagttaaagc catccgcgtc
    30181 agccaagatt gtctccacat ctacagcgtc atgcgactgg tatcctaccc gacatagagc
    30241 cttccaactt gggcaagaca ggaaaaccta tttggtcagg caaagtattc cgaaaacgtt
    30301 gaacctactt gtcttggcaa aattgtcggt tacactagaa cacttcctgc caaaccgttg
    30361 accggtttga aatcacccgc atgatgcaca ggcaagccga ccttgcctat cgaaacggac
    30421 atcctccctt gcgttgtttc ttgaagggct ttgcccacgc atcgtttggc gaaaggactg
    30481 ttgagaggag gagtgtttcc atgcgtgccg ccttggttgt tgcgatcgtg ttattgggga
    30541 tgaatccgat tggccgtggt tcggagcctg tttcctattc tgagcaattt gcctattctg
    30601 ctgcgtatcg gcaaagcctc gaaacccagc aacccttttt ggtcattgtg ggagcacagt
    30661 ggtgtcctgc ttgccgaaaa ctcgagagtg aagttattcc caaaatcaaa gagatgggcc
    30721 ttacaaagca ggttgccttt gctgtggtgg attacgacgc cgagaccaag gtggcccagc
    30781 aactcacccg gggtggcccg attccccagg tgctcgtttt tcgccgcgga gagcaaggct
    30841 ggaaatcgga tcggctcgtc ggatatcctt cacaaaaggc agtcatctcg ttcattcagg
    30901 cggccctgtc atcaaaagat caagcgccgg ccggccaaca ggcaagcaac aactgagcag
    30961 acttttgctc ttggccggaa tagagaccct cggcataggc aagcacaaga cgatgcttcc
    31021 accgatcgaa cgacacaaca tccgccaact ggaagagctt aatcagcgcg gcggccgtat
    31081 gctgacggtt gtggatctga ttgaggctgg gactatctcg cccgaaatgg cagccgccgt
    31141 tatggaagcc ttggcccgag gggcctcgat cctgacaggt gcgcgtccga gcggtgcggg
    31201 caagacgacc gttctggccg cctttctcaa cctactcccg ccggatgtca ccatcgtcac
    31261 ggttgatgac ccggccgtcg ttggacaggc tttggcacgg ccacccgaac atccgacctg
    31321 ttatctggtc cacgaaatcg gcgccggcca ctggtatggc tatctttggg gacaagccgt
    31381 ctctgactac ttgcgcttga ccagaggtcc tcatcgcatc gcctcgtgcc tccatgccga
    31441 taccctggag gaactcactc acatcctgac tagcccacca ctttgtgtcg atccatccga
    31501 tctgcatcgg gttggtatct tgggatttat gcggatggac
//
LOCUS       DAOSUT010000028        31134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1002_length_31134_cov_10.177483, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000028 DAOSUT010000000
VERSION     DAOSUT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 31134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31134
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_31134_cov_10.177483"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..980)
                     /locus_tag="PLD05_04095"
     CDS             complement(462..980)
                     /locus_tag="PLD05_04095"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOP76644.1"
                     /translation="MTLSEELERLHRLHECGALTDEEFAAAKAQLLKNSADLKPPPLV
                     FPRLRDRLQSGRFWAMLLHLSLLLNFLMPGVALIITFLIWQIGKDRFAELDEHGKNVI
                     NFVLSYGIYALILFLLFIIPVVSIPVIGAAIVMPMFYLFMAIVALLALFAAWKANQGQ
                     VWRYPLCFRFFE"
     gene            complement(1082..1918)
                     /locus_tag="PLD05_04100"
     CDS             complement(1082..1918)
                     /locus_tag="PLD05_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76645.1"
                     /translation="MNARDAQHPAVETVVPEAAAYQPLSVAAVVALLLGLVSFTALLT
                     PILWAVAFAGVVVSIFAWVRIHRFDPPLTGKTAAIIGLVLSLFNSAAGPAHWYVRRTM
                     LVQEARQVAELFLDFLKQNQPHKAHQLSLAAAQRSPLDDNLWTKYAPGTSARKDLEDF
                     LERREVRAIILLAKDPRTLVRFFDVEGVWRENDEDRVALSYAVTYVDPQDKKRTFFVE
                     IALNRYSMRTQPVSDWYISNISSLTPPRTLEAEKAAWDEKQKKQRPLAANPDDETPEP
                     IE"
     gene            complement(2012..2656)
                     /locus_tag="PLD05_04105"
     CDS             complement(2012..2656)
                     /locus_tag="PLD05_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOP76646.1"
                     /translation="MWLLMLIQIVIIAACVGFLYREGMWGNFIRFVNVLFAAVLATNF
                     FEPLARILEELISPKLTYFYDFLAFWLVYAVSFLVLHVATAAASRVKVRFNHYLNMGG
                     GIFLALVIGLTMCSVLKFSLHLAPLGTKPFLASVVDEDGDPLGAQWAGIMRGLSSGVF
                     SRSVAAEAEGIYGGSVAKFPGAKNIFEVYLERAKALEAQVNNGGGFTTEGAPPR"
     gene            complement(2749..4533)
                     /locus_tag="PLD05_04110"
     CDS             complement(2749..4533)
                     /locus_tag="PLD05_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOP76647.1"
                     /translation="MDILMINSKSLVLRRLAWVIVLVVIASGWADTVLLAAPWFSPWL
                     TVGQAGSGWSGPGHYINFWKFLAFFLVFLFWVKAGDWVSQDSQRLDLRPNTWNTVIFF
                     VFVAAFVSGLLIPVFPVSLLLVLLAAIVPFAIYVVQRNGKVGAEERVFTVLHFKQIIG
                     RLSGKKVVVPLSPDDLPPPIRFEPIAKTQQEKNLRLIPARQHPGYQAAREFIAQTLDR
                     RGEAVDLMVAPTATTMRYMIDGTWVNMPPIPPETAMGAVEVLKILCGLNPQDRANRQQ
                     GKLDLKYHDVPLSLTFASQGVTGGERVVIQFVEKKPRFRKLEDLGMRPKLQEMIKSFL
                     ETPQGIIVFSAPPGHGLTTTVTCALLTGDRYSREWISIEDEQKTYEVIENVQPHIIKA
                     EDRQNLEQVLDKIFHLEPNVVVYRDVPDGKVLERLAQETENNRLIITTVRAKDCCDAL
                     QKLMALPPPSKTFPEKVIGVVNQRVMRRLCDKCRQPYAPPAELLQRLGIPPGRIQAFF
                     RPRVPTPEDKEVCPQCQGLGYFGRAAVFEVLAVNDNLRKLLARRPDYATMRKAARAAG
                     MATLQEEGVLLMAQGITSYEELMRVLQG"
     gene            complement(4533..5681)
                     /locus_tag="PLD05_04115"
     CDS             complement(4533..5681)
                     /locus_tag="PLD05_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOP76648.1"
                     /translation="MATTQETVRPVFGKRPRNLEIDKLFRALVKFEGSDLHLKVGRPP
                     FVRVQGSLRPLNLPPIDEDEMVRLILPMLDERLRRILEEEGGCDFAHVVDVDGVSWRF
                     RVNVLQQMGYLGLVARRINNYIPDFEKLNLPPILENLCKFDQGMILLAGVTGSGKSTT
                     IASMLNWINRHYRKHILTLEDPIEFTFTEDKCLINQREVGIDVKNFIIGMKHAVREDP
                     DVMLVGEMRDRETFETAIQAAETGHLVFGTIHASSAPSTISRILDLFPQSMHRAIRSA
                     IAFNMKAIIAQKLLPSINPEVKRVPAVEIMIFNPMVKKLILEEQDQKLADIIRMSKEE
                     GMQDFTMSLKSLVERRLIDRATAFEVAPNPEALQMALKGIEVKAPGII"
     gene            complement(5834..6601)
                     /gene="hisF"
                     /locus_tag="PLD05_04120"
     CDS             complement(5834..6601)
                     /gene="hisF"
                     /locus_tag="PLD05_04120"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOP76649.1"
                     /translation="MLAKRIIPCLDVDRGRVVKGTRFVNLRDAGDPVEVAARYEEEGA
                     DELVFLDITASHEGRDIILDVVRRTAEVVFMPLTVGGGIRTLDDIRRLLNAGADKVSI
                     NSAACRNPDFVYQASRRFGSQCIVVNIDPKRVIKDRREFWEVHINGGRVGTGLEAVAW
                     AKEVERLGAGEIVLTSMDADGTQKGYDLEITKAVADAVTIPVVASGGAGCPEHMADAI
                     LKAGADAVLAAGIFHFGQFTITETKQYLKRCGIPVRL"
     gene            7053..8033
                     /locus_tag="PLD05_04125"
     CDS             7053..8033
                     /locus_tag="PLD05_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOP76650.1"
                     /translation="MVTLKKICGALVLGILIGWWTPSVAQVQVDPQLPDYKPVQGISG
                     NLKSVGSDTMNNLMTLWAEGFRRFYPNVQIEIEGKGSSTAPPALIAGTAQFGPMSRPM
                     KSAEIDQFEARYGYKPVALVTAIDMLAVYVNKDNPLKGLTLQQVDAIFSKTRRGGYPH
                     DIRTWGQLGLTGVWSQQPISLYGRNSASGTYGFFKEHALFGGDYKDEVKEQPGSSSVV
                     QGVASDRYAIGYSGIGYKTADVRALPLAPDANSPFVPAEPDHAYTGDYPLARFLYVYV
                     NYKPGTTLDPLRAEFIKFIFSKQGQAEVIKDGYFPVPAVIAKKSLASVGL"
     gene            8106..10766
                     /locus_tag="PLD05_04130"
     CDS             8106..10766
                     /locus_tag="PLD05_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOP76651.1"
                     /translation="MTTVHRRKTSWATKFVDRLAGWLITLGGIGTICAVVGVCVFLVW
                     VVLPLFKPARLIPIHFVAQSANESSGGSVAPSGNQTVPFDAKLLAVGLDETMTIIWSV
                     DEDGNLIVQQFADQDVLFKKDLSSHGRPRTLAVDTRSGYIAFGYDQGRIQFTRVHVAV
                     TPAQIEDVPIDIREQVTGKPPYKALSGDGACWFAQFGNIYRLEVRVEEEPDVTVGEAA
                     VDRLAFSLRAEVRNIAALTADGILHIGRTDKKVNFLTGEESVRFTGGALNLQDAGISA
                     IPDFLQISGVADSTYLIWRNGRTLRLDTRDLTNPRIAEEVSLLSPGEGEISAVSFLIG
                     KMTLIVGSTTGTVSAWFPIKPAHAETADGVHLVRAHTYRPGQGAVVALGVSHRSRLFA
                     ALFADGTVRVYHATTEQVVTEGCISEVVPVAGQELRDLPATGIALAPKDDGVFAVLGG
                     HPSLWRLVAPHAGVTWKSVFGKVWYEGYEKPAFVWQSSGGTDDFEPKYSLVPLIFGTL
                     KATVFAMLFAVPIAFLAAIYTSEILPREVRSVVKPTIELMAGLPSVVLGFLAALVFAP
                     WVESRLLQVLSLFYVIPFTLLLGGHLTNLCPHNWQQIVRRYRAWFIVLAAGVGVILAQ
                     WVGPTAETWLFSGDIKAWLDGRTGRGEPGWVLPLLPLTSLAVGWMVLRVVEPMIEGRR
                     PAISRTAWAALKLALFIVGAVVAVALAWAIGWLLVSGIGLDPRGLFLGTYIQRNAFIV
                     GFVMGFAVIPIIYTIADDALTAVPDHLRAASLGAGATPWQTAIRIVIPTAMSGLFSAF
                     MIGLGRAAGETMIVLMAAGNTPIMDWNIFNGFRTLSANIAVELPEAVQYSTHYRMLFL
                     AALCLFVMTFVINTLAELVRLKFRKKAYQL"
     gene            10838..12601
                     /gene="pstA"
                     /locus_tag="PLD05_04135"
     CDS             10838..12601
                     /gene="pstA"
                     /locus_tag="PLD05_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOP76652.1"
                     /translation="MAQSVVVSLKEKINNQWRDRPRSQGSLLSAYGEPAIWLTGGALV
                     ICLAMILGLLLLILINGLRTFWPARVVHIKLRNGQAVLGEITRSEWYRPDPAFLEQLS
                     PDQRALYVEAIEAHDGWVKRELIRIGNYELTNEHFRWVALPEIAPGGGTFPDWALVIE
                     RVTWGRFYGFPVGFVVGETVTATEPQQIWQEFQKHHPRVRQRWRRIRQLETRTLGDIN
                     YALEKSRLRLRTVELREGKSSPAYQELAQQFARQEAELQQEYDSVRTEIESLRRENSQ
                     YGLRLRTADGQEKDIGLADIVRAYPANQLSLGERLALYGSRVWEFLSDEPREANSEGG
                     IFPAIFGTAFLTLLMSLAVVPLGVVAALYLREYAKAGVVVSTVRVAVNNLAGVPSIVF
                     GVFGLGFFCYVVGGTIDQVFFSARLPNPTFGTGGILWASLTLALLTMPVVIVATEEAL
                     AAVPRSMREGSYACGASRWQTIRRIVLPRAMPGVLTGAILAMARGAGEVAPLMLVGAV
                     KLAPELPLDHRFPYLHLERSFMHLGFHIYDLGFQSPNSEAAKPMVFSATLLLILLVAA
                     LNLGAMVLRSRLRKEMRATHL"
     gene            12914..13690
                     /gene="pstB"
                     /locus_tag="PLD05_04140"
     CDS             12914..13690
                     /gene="pstB"
                     /locus_tag="PLD05_04140"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOP76653.1"
                     /translation="MHAEFAQETPILEIDHFSLSYGPKQVLFNISMIIPRGKVTAIIG
                     PSGCGKSTLIRCINRMNDLIESVRIEGDIRLDGTSVFDPAVDVTEIRKRAGMVFQKPN
                     PFPMSIFENVVYPLRIQGVRDRRVLAEVCERSLRAAALWDEVKDNLHQNAMQLSGGQQ
                     QRLCIARALAGDPEVLLLDEPCSALDPIATNKIEDLIRELAGTYTIVIVTHNMQQAAR
                     ISDFTALLYLGRLIEYGPTRRIFSAPHLPETEAYVTGRFG"
     gene            13776..14060
                     /locus_tag="PLD05_04145"
     CDS             13776..14060
                     /locus_tag="PLD05_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76654.1"
                     /translation="MTLSGIDLLSFVVLAQEGTSATSWAPGYLVTILVLLFVMMVTLA
                     PSRRADKPKQFEFTGTSWGKVVTGETEKKQEPQGPPPGVFAPGQLAQKRK"
     assembly_gap    14292..14391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14392..15293)
                     /locus_tag="PLD05_04150"
     CDS             complement(<14392..15293)
                     /locus_tag="PLD05_04150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP76655.1"
                     /translation="MQPEQGAATPSFESESHRIPVPFLDLARSHRPLRDQIVEALARV
                     CDSGAFVLGPEVEHLERILAQYCHCEHAIACASGSDALLLALMALGIGPGDEVIVPSF
                     TFFATASAVTRLGAKPVFADIDPATYNISPREVARLIHKNTKAIIPVHLFGQAAEMEP
                     ILRLAQQHHIFVIEDAAQSIGAEYHQRRVGSLGQIGCFSFYPTKNLGAMGDAGMITTH
                     DAELAEKLRVLRVHGMKPRYYHHWIGINSRMDAFHAATLLVKFNCLETWIAERQQIAD
                     RYYQLFAEYKLAQVILPQVLPGRR"
     gene            15555..17336
                     /locus_tag="PLD05_04155"
     CDS             15555..17336
                     /locus_tag="PLD05_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76656.1"
                     /translation="MQQRSFVNIFGLIFLPWAIGCLLASVVGYVGLTWQERSLKQAQA
                     EEWERFISGLIEGEIGSQPKITDLQESRLLQSLIQNTRLELAVFTASGKYVVGSVTNP
                     PPNIPSEVQMVLKTSQRFTTWTEKRGQGRPQLRMAWPRQVEGKTHIIWAAIDLGTVTG
                     NGPSLRGLLAWNVVFFAIGLPFAWGLTWWLRRRLDVVTKSALEGEPSLRPWDLWEWHA
                     LAHRLRDYVAQQRQQIEVLQGIARDHQAVLEHLPDGLLFVTPAGRVAKHNPAAGQLLR
                     FPGTLAEGLSIRECLRKPEFLTWVEDLLSLKRPPVLSLHNDDPEQHLEIRGAELKGPD
                     QRVHGFLLIIRDMTRTWAADRVRRDFVANVSHELRTPLTSIKGFLETLLDGALEDPPQ
                     ANRFVRIIHEQTERLERIVNDLLILARLEGEHEQPITREPVRVEELVLGAVDACRYQA
                     EQRGMQLHWEVPKDLTIRVNGRLFHQALVNLLDNAIKYSDPNKEIRILVRPGEKELIF
                     EVQDQGWGIEARHLPRIFERFYRVDPSRSRQLGGTGLGLAIVKHVAQIHGGRVTVDSR
                     VGVGTTFRIHLPVEHVLHERPTDRQSE"
     gene            complement(17348..17914)
                     /locus_tag="PLD05_04160"
     CDS             complement(17348..17914)
                     /locus_tag="PLD05_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76657.1"
                     /translation="MVAKIEIPCPCGKSVPVTINQCGMIVTCSCGRELAVPSRRELLG
                     TRQTIDEDASVEEEKAPLSGWVRLFQWISGSLAVLALIGLIVLYIYRPRLPDFHKLPP
                     AAAYQYFKVLRTGERSPWLEMEARYVRDHNIWLGLRDIVAVVGIIAIVLFCGVTLAGW
                     WETRSNRKEEDEIVEPPGAESEGTESPS"
     gene            18287..>18735
                     /locus_tag="PLD05_04165"
     CDS             18287..>18735
                     /locus_tag="PLD05_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOP76658.1"
                     /translation="MDNLLGDNLRRIMSQLGLTINEVAERTKLDKRTIRGILEGSKRP
                     HPKTIGQLAEGLGVSVDELYIDPTRLLYRRLDKQTNPVVEKVIQEYPELFADWTTAEF
                     EELYSRFGMGGAMTEEGVLEAARKTNRRRILQHKLAVLLETSQAELI"
     assembly_gap    18736..18835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18836..20298)
                     /locus_tag="PLD05_04170"
     CDS             complement(<18836..20298)
                     /locus_tag="PLD05_04170"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HOP76659.1"
                     /translation="MDHEAQPVKRSQVEKLKEESQYLRHPLAEEVNNDQPSFSESAAQ
                     ILKFHGSYQQDDRDARKLQRASGGGKVYFTMIRVKVPGGVLTAEQFLTQLDLCDQLGN
                     GTARITDRQDLQLHGVLKGNLRETIRRINEVQLTTLGACGDVVRNVMCCPAPLRHNRV
                     REQLQNLAQEITRHLLPRTPAYHEIWLTDPDTGEKEQVTEPSGEVAEVEPIYGKTYLP
                     RKFKIAFGLPDDNCVDLYTNDLGFLAVVENGEVVGFNVLVGGGLGVTPSNKNTFPALS
                     QPLVFVRPAEVVPVAEVVVKIYRDYGERGDRKRARLKYLIADRGLPWFREMTERYLGR
                     SLPDPLPLGVTEVDDHMGWHEQGDGRFFYGLNLENGRIVDRDAFRLKSALREICQRLH
                     PSIRFTPQQSVLFCDLEASARSEIEQILRRYGVPLSEEISNVRRWSMACVALPTCPLA
                     VTESERVLPKLIDDLEREVAKLGLDAERFTVRMTGCP"
     gene            complement(20492..21199)
                     /locus_tag="PLD05_04175"
     CDS             complement(20492..21199)
                     /locus_tag="PLD05_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOP76660.1"
                     /translation="MNAAFRLQVADGCLGIKCCSDCPHPRCLRDRFLIGAAVASGVLQ
                     RPTLVLNRNWQPIHVATVARALVMVYQGTALVVDPEDFSTYSWSDWAQLAPADGESFI
                     QGVSFRLRVPEVITLREFDRVPYSGVPFSRRNLFKRDHNTCQYCGRQFPPDQLTIDHV
                     IPRARGGESTWENCVLACVDCNRRKGNRTPEEAGMRLKRRPTRPKWRPVYAAKEIRIE
                     TWTRFVSEAYWNVTLEK"
     gene            21559..22311
                     /locus_tag="PLD05_04180"
     CDS             21559..22311
                     /locus_tag="PLD05_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOP76661.1"
                     /translation="MAGHSHWASIKHKKAAVDAKRGKIWSKVSKAIIIAARIGGGDPK
                     MNPRLRVAIEEAKAVGMPKDNIMRAIKRGTGEIEGAALEEVTYEGYGPGGVAIMCDVL
                     TDNRNRTAAEIRKCFELHNGKLGGAGCVAWMFETKGLFLIPADQVNEEQLMDVALEAG
                     AEDIKQVGDKFEVTCDPAKFSDVSEALDRANIPCDSRSVTRIPKDYVDLDVETARKVL
                     KLMEELDEHEDVQNVSSNFNIPDEVMAELSRS"
     gene            22357..22779
                     /gene="nikR"
                     /locus_tag="PLD05_04185"
     CDS             22357..22779
                     /gene="nikR"
                     /locus_tag="PLD05_04185"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOP76662.1"
                     /translation="MSKIVRFSISLERDLLKKFQRFCREERFATRSEAIRQLIHDFLA
                     RRAWVEDAEDVAGTLTLVYDHHRSQLRDQLMKLQHDHTDLIVSTLHAHLTAHLCLEVI
                     ILRGPAKQLREIAARLRGLKGVHKGELVIAAVPQESPA"
     gene            22980..24080
                     /locus_tag="PLD05_04190"
     CDS             22980..24080
                     /locus_tag="PLD05_04190"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOP76663.1"
                     /translation="MLKGNAIVGQSGGPTAVINASLCGVIEGCRESSQIERCLGMRFG
                     IQGFLESDVIDLDRESAETISRLRTTPSSALGSCRYKLRDEDFPRLLEQLKRYNIRYF
                     FLIGGNDTMDTIHRLEEFCRKANYPMLGVGIPKTVDNDLFGTDHTPGYGSAARYVAIS
                     VRQAGMLARDMKRVDQFVIFQTVGRSAGWLAAASALARSAEDDAPHLILLPERPFDRD
                     RFLAAVKQTYDKYGWCSIVCGEGITYPDGTPVSAARVTDQFSNIEFGAMGGTSAAMML
                     HRMISQAFGWRGEFQVCESLQMCAADRVSDIDLEEAYACGREAVRLANEGVSGMMVSI
                     QRESSSPYRYRLGVVPLAEVAIKAKPMPDEFY"
     gene            24244..25287
                     /locus_tag="PLD05_04195"
     CDS             24244..25287
                     /locus_tag="PLD05_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOP76664.1"
                     /translation="MELQLIRKEQAQPPFFVGVDLGGTNIKAGVTDDRGAVLWVGQEP
                     THVERGPEDGARRIADLIHRAIHEAGLTPQDIAGVGLGSAGTMDIPGGRLVVPPNLKG
                     WEYFPIRDRVSEHTGMPITFANDATAAAFGEKWVGAGHRFHSMILLTLGTGIGCGIIV
                     GDLLLDGEHSHGGEYGHTVIDCRPDARVCSCGRRGHFEAYASATAVTKRAEEALAQGR
                     VSMLTRRIQEGAKLDPKLIAEMAEAGDELSRQLILETAEYLGIGIVNLMHTIDPNGIL
                     IGGAMTFGGKESRIGRIFLERVKHEIFLRAYPAVGDTTEIDFATLGGNAGFIGAAGIA
                     RLEKMKAQFPPAK"
     gene            25327..26475
                     /gene="proB"
                     /locus_tag="PLD05_04200"
     CDS             25327..26475
                     /gene="proB"
                     /locus_tag="PLD05_04200"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOP76665.1"
                     /translation="MTIDLVRQEIATEARLIVVKVGTRVVTHPDGRLNYERIEQLAEE
                     IHCVRQTGRKVVLVSSGAVGAGVGRLGLPGRPTDLSELQAVAAIGQSLLVEAYDQTFR
                     RYNDVAAQVLLTADVIENRTSYLNARNTLLALLNRFDAVPIINENDTVAVEELRMTFG
                     DNDRLAALVTNLLQAQLLVLLSDVEGLYSGDPNDPKSELIPLVRKIDDSIFDLVRDKK
                     TGLSKGGMASKLKAAQLVTTGGGNVIIASGKTPGVLKKIIAGEVVGTLFFAKGPTVAA
                     RKRWFGLSIQPRGQLIVDAGAREAIEKRGKSLLPIGILDVVGQFQKGDLVALRDPDGC
                     EFGKGLSNYSAEDVRRIKGLRTRQIAQVLGWLPYEEVIHRDNLLVTRP"
     gene            26781..28574
                     /locus_tag="PLD05_04205"
     CDS             26781..28574
                     /locus_tag="PLD05_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76666.1"
                     /translation="MAMDDKLPNSHQPKPPPLGAKEKPADKPELDPKTSAPVESQPSG
                     AAAQEKTVSGRKVTRSPKAENSQATTTQGISAAAQQREPSSAQAAVQSQEERPRSRVT
                     TLLVEILHWMPSWFVSMVVHAVALLVMAMITIPGPMQDAVQQLVVAPGENEQLEEIEE
                     LLTEPPEDINLATDLSPVEVASTIDSSQDISPFNDEAAAMASFELNPIGLEHAPQTDL
                     LASIGALSGDAFSGRGSGKERMVRTAGGSEGSERAVALALKWLADHQMPDGGWSFEHQ
                     KAGGCNGQCRNPGNLPDARAAATALALLPFLGAGQTHREGQYAKNVRAGLYYLITNRM
                     KISPEGGSFLEKGGNMYSHGLAAIVLCEAYGMTHDKVLAAPAQQAINFICNAQDPVGG
                     GWRYQPRQKGDTSVVGWQLMALKSGAMSYLQVPKVTIQKAQAFLDSVQANDGATYGYL
                     DPGADPGTTAVGLLCRMYLGWDKNHPGLKKGIAQLSAWGPSTDKSGPSKNNFYYNYYA
                     TQVLRHWEGEEWKKWNSVMRDYLVNTQAQKGHETGSWFFEDCSDHEKERGGRLGGRLY
                     FTAMATLILEVYYRHMPLYRKQATAENFPLD"
     gene            28699..29343
                     /gene="lexA"
                     /locus_tag="PLD05_04210"
     CDS             28699..29343
                     /gene="lexA"
                     /locus_tag="PLD05_04210"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOP76667.1"
                     /translation="MGKNDIAALSSLTPRQREILDFIRDWIEQFGYAPSVRDIGRHFG
                     IRSPNGVRGHLKALEAKGVIERDPRRSRALRLTPAARGRVSRSGKGFTLPLVGRIAAG
                     QLHEAIENAETFDFQSLFSSKSADHFVLRVHGDSMIEAQIADGDYVIVRRQETATPGS
                     IVVALTDENEATLKYWYPTKRGAKLVPANKRMAPIFVKNAKVLGVVVGVVRKLA"
     gene            complement(29474..31045)
                     /gene="purH"
                     /locus_tag="PLD05_04215"
     CDS             complement(29474..31045)
                     /gene="purH"
                     /locus_tag="PLD05_04215"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOP76668.1"
                     /translation="MSMIVKPRRALISVSDKSGLGDFARGLCDFGLEIFSTGGTRRFL
                     QEQGIPVTDVAAYTGFPEMMEGRVKTLHPKVFGGILARRDHADDVRALEEHHILTFDV
                     VVVNLYPFRQTVARPNCTEADAIENIDIGGPSLVRAAAKNHAFVAIVTSPAQYELVLK
                     ELREHGGTTLALRRQLAAAAFAHTAEYDQAIATWFAQRVEEGPFPSRLFFTFERWTPL
                     RYGENPHQQAAAYRMPEVHGPSVVTARPLHGKELSYNNLLDLDSALEIVRHFREPAVA
                     VIKHNNPCGAAVAATLAEATRNALAGDPVSAFGSILGMNRTVDAETAEVLSEPGLFIE
                     AIIAPHFDAKAFEILTSKPKWKKNVRLLEVGDLTEERERWAYRHLTGGLLVQEADNLA
                     DDESQWQVVTQVRPDESLLPDLRFAWAMVRFVKSNAIVLARNGMLVGVGAGQMSRVDS
                     VEIAIRKAQDRAQGSVLASDAFFPFPDSIEHAAQAGIRAIIQPGGSVRDEEVIAACNK
                     HGLPMIFTGRRHFKH"
BASE COUNT         6997 a         8774 c         8476 g         6687 t
ORIGIN      
        1 gtctcgattt cgaggaactc cgccagcaca tcccgctgag tcagtgcggc cagggtggcc
       61 gacagaccaa tcgtcctgtg ggccgaatgg aaaataaacc gtcgccgatt gactcgattc
      121 atcgtctcct cccgctagat ccgatgcccc gcacccaggc cccatccacc caatctgttc
      181 tttttagatg ctccacgtct ctattattca ccccgtcgtg gcccgtgtcc aataagagag
      241 acaggtctcg cgcccgtttt gcctcgactg agaacgacag taagagctcg gcaggatagt
      301 tgctccaagc gggttgggag agggacggaa aaccatgaag ttatagagaa tgtaacgcca
      361 aacttaccgg ccctcctgat ttcccgatcg caaccccaaa acttttccca atctgaatcg
      421 ttcctctttt ggttaacgtc attgtcttgt gtaaaggtgc ctcattcaaa gaagcggaaa
      481 cacaacggat accgccaaac ctgtccttgg ttggccttcc acgccgcgaa caaagccagc
      541 agcgcgacaa tcgccatgaa cagataaaac ataggcatga caatcgccgc accgataact
      601 ggaatgctga caaccggaat aataaacagc aagaaaagga tcagcgcata gatgccgtaa
      661 gacaagacaa aattgatcac attcttgcca tgttcatcca attccgcgaa gcggtccttg
      721 cctatctgcc agatcaagaa tgtgatgatt agggcgaccc ccggcatcaa aaagttcagt
      781 aacagggaca aatgaaggag catggcccaa aatcggccgc tctggagccg atcgcgcaaa
      841 cggggaaaca cgagcggcgg tggtttcaaa tccgcgctgt ttttcagaag ctgggccttt
      901 gcggccgcga actcctcatc agtcaatgca ccacactcgt gaaggcgatg gagccgttcc
      961 aattcttccg aaagggtcat gatcatgatc ctcccctgct cttgggaatc accacagtcc
     1021 ttacacgacc cgttgggaga ccactttagg ccacaccaag gttgcccaac tcgtggtctc
     1081 tttactcgat cggctccggt gtctcatcat cggggttggc ggccaaagga cgttgctttt
     1141 tctgcttttc gtcccaggcc gctttctccg cctcaagagt tcgcggcggc gttaaactac
     1201 taatgttgct aatataccag tcagagactg gctgcgtcct catggaatag cgattgagag
     1261 cgatctccac aaagaacgtt cgcttcttat cttggggatc gacgtaggtg acagcatagc
     1321 tgagcgctac ccgatcctcg tcattctccc gccaaacccc ctccacatcg aaaaagcgaa
     1381 ccaatgtccg gggatccttc gccaagagaa taatcgcgcg aacttctctc cgttcgagaa
     1441 aatcctcgag gtcttttcgg gcactggtcc ccggggcata cttcgtccag agattatcat
     1501 ccagcgggga gcgctgggcc gccgctagac tcaactgatg ggccttatgg ggctgattct
     1561 gtttcaggaa atcgaggaac aattccgcca cctgccgggc ttcctggacc agcatggttc
     1621 gccgcacgta ccaatgggcc ggcccggccg cagagttgaa cagactgaga accaatccta
     1681 taatggcggc ggttttgccc gtcaatggtg gatcaaatcg gtgaatacga acccaggcaa
     1741 agatgctgac cactacccct gcaaaagcca ccgcccaaag gatcggggtt aacaaggccg
     1801 taaaagacac caaccccagc agcagtgcca cgacagccgc gacacttagg ggctgatacg
     1861 cagccgcttc gggaactacc gtttccaccg caggatgttg ggcatcgcga gcgttcaatt
     1921 tgccatctcc ctcatctctt accttgtcat ggccccacac tcggtaccca ttttcaagtt
     1981 aaccagccac gtctacgatc agcacagatc attaccgcgg aggagcccct tcggtggtaa
     2041 agccaccacc attgttcacc tgggcctcca acgccttcgc ccgttccaag taaacttcaa
     2101 aaatattctt ggcacccggg aacttggcaa ccgagccgcc gtaaatgcct tctgcttcag
     2161 ctgccacact ccgcgaaaag acgcccgaac ttagcccccg catgatcccc gcccactgag
     2221 ccccgagagg atccccatcc tcatcgacga cggaggccaa aaaaggcttt gttcccaaag
     2281 gggccaggtg cagcgagaac ttcaacacgg agcacatggt taggccaata accaaagcga
     2341 ggaaaattcc gccacccatg ttaaggtaat ggttaaaacg gacctttaca cgggacgccg
     2401 cggccgtggc cacatgcagg acaagaaacg atactgcata caccagccaa aaagccagga
     2461 aatcataaaa gtaggtcaac ttgggactta ttagttcttc caagatcctg gccaagggtt
     2521 caaagaagtt cgttgccaaa acagcggcaa acagaacatt cacgaaccga atgaagttac
     2581 cccacatccc ctctcgatat agaaatccca cgcaagccgc aatgataaca atctgaatta
     2641 gcatcagaag ccacatcgtc ggtctccaca actgttagcc ctcggtcatc cgcgttcacg
     2701 agggacgtga tggcacgatt cgctttccta atcttcaaac ttttctcatc aaccctgaag
     2761 aacccgcatc aattcttcgt acgaggtgat tccttgagcc atgagcaaga cgccctcctc
     2821 ttgcaaggtc gccattcccg cggcccgggc ggcctttctc atggttgcat agtccggtcg
     2881 ccttgccaac agcttgcgca ggttatcgtt gaccgcaagg acctcgaaga cggcggcacg
     2941 cccaaagtat cccaacccct gacactgcgg acacacctcc ttatcctccg gggtgggaac
     3001 acgcggccgg aaaaaggcct gaatccgccc tggcgggatc cccaaccgct gcaataactc
     3061 tgccggtgga gcatacggct gacgacactt gtcgcacagt cgtcgcatca cccgctggtt
     3121 gacaacgccg atgacctttt ccggaaaggt cttcgacggc ggcggcaaag ccatcaattt
     3181 ctgcagggca tcacaacagt ccttggcgcg gaccgtggtg ataatcagcc gattgttctc
     3241 cgtttcctgg gccagccgct ccaagacctt tccatccggc acgtcgcggt aaacaaccac
     3301 attaggttca aggtggaaaa tcttgtccaa aacctgttct agattctggc ggtcttcagc
     3361 cttaatgatg tgtggctgaa cgttttcaat tacttcgtac gtcttctgtt cgtcctcgat
     3421 gctgatccac tcgcgcgaat accgatcccc agtcagcaga gcacacgtga ccgtggtcgt
     3481 caggccatga cctggtgggg cagaaaagac aatgatcccc tgaggggtct cgagaaaact
     3541 tttaatcatc tcttggagtt tcggtcgcat ccccaagtct tctagcttgc ggaatcgcgg
     3601 ctttttctcc acgaattgga taacgacacg ctcgccaccg gtgacgccct gcgacgcgaa
     3661 ggtcaaactt aacgggacgt cgtgatactt caaatcgagt ttcccctgct ggcggttggc
     3721 acggtcttgt gggttaagtc cgcacaaaat cttcaggacc tccacagccc ccatcgctgt
     3781 ctccggcggt atcggtggca tattcaccca agtaccgtcg atcatatagc gcatagtggt
     3841 ggccgtaggg gcaaccatca gatcgacggc ctctccgcga cgatccagcg tctgggcaat
     3901 gaactcgcgg gcggcctgat atcccggatg ctgcctggcc gggatcagcc gtagattctt
     3961 ttcctgctgc gtcttggcaa tcggttcaaa tcgaatgggc gggggaagat catccggcga
     4021 cagcgggaca acgaccttct tgcctgatag tcggccgatt atctgcttaa aatgcaatac
     4081 ggtaaaaaca cgctcttcag cccccacttt accgttgcgt tgaaccacat agattgcgaa
     4141 cggtacgatg gccgcaagga ggacgagcaa caaacttaca ggaaacaccg ggatgagcag
     4201 ccccgatacg aaggccgcca caaacacgaa aaaaatcacg gtgttccatg tgttcggtct
     4261 taagtccaga cgttgactgt cttgactaac ccagtcaccg gccttaaccc aaaacaaaaa
     4321 cacgaggaaa aatgcaagaa atttccagaa gttgatgtaa tgccccggtc cggaccaacc
     4381 actgcccgct tgacctaccg ttagccaggg agaaaaccag ggagcggcca gcagaacggt
     4441 atcggcccaa cccgaagcga tcacaaccaa gacgataacc caagccaacc ttcgcaaaac
     4501 caagctcttg ctgttgatca tgaggatgtc catcaaataa tcccaggtgc cttgacttcg
     4561 atccctttca aagccatctg cagggcctcc ggattggggg caacctcgaa ggccgtcgcc
     4621 cgatcaatca gtcgccgctc caccagactc tttaagctca tcgtgaaatc ttgcataccc
     4681 tcttctttac tcatccggat aatgtcggcc agcttttgat cttgctcttc caggatgagc
     4741 ttcttgacca tggggttaaa aatcattatc tcaaccgccg gaacccgttt gacctctgga
     4801 ttaatcgacg gcagcagttt ctgggcgatg atggccttca tgttgaaggc gatcgcgcta
     4861 cgaattgccc tgtgcatcga ctgtgggaac aagtcgagaa ttcggctaat cgtactcggt
     4921 gcgctggagg cgtgaatggt cccaaaaacg aggtggccgg tttcagcggc ctggatcgcc
     4981 gtttcaaacg tttcgcggtc acgcatttca ccgaccagca tcacgtcagg gtcttcacgc
     5041 accgcgtgtt tcatgccgat gatgaagttc ttcacatcaa tcccaacttc ccgctgattg
     5101 atcaggcatt tatcctccgt gaacgtgaac tcaatcgggt cctcaagtgt gaggatatgc
     5161 ttccgataat gccgattgat ccagttgagc atggaggcaa tcgttgtact cttccctgat
     5221 cccgtcacac cggctaggag gatcataccc tgatcgaatt tgcaaaggtt ttccagaata
     5281 ggcggcaggt tcagtttctc aaaatcgggg atgtaattgt tgatccgtcg ggccaccagt
     5341 cccaagtaac ccatttgctg gagaacatta acacgaaagc gccatgagac gccatcgaca
     5401 tctaccacat gggcgaagtc acaaccgcct tcttcttcga ggattctgcg taaccgctcg
     5461 tcaagcatgg gcagaatgag ccgcaccatc tcgtcttcat caatcggcgg taagttcagg
     5521 gggcgaagcg acccttgaac ccgcacgaac ggcggccgtc ccaccttcag gtgtaagtca
     5581 ctcccttcaa acttcaccaa tgcccgaaac agcttgtcga tttccaaatt acgcgggcgt
     5641 ttcccaaaaa cgggacgaac ggtttcctgt gtggtcgcca taatgtgacc tcgattcccc
     5701 gaatttccga aatacccacc ttgcgcgcca tgacgtatcc tgcgcgctaa gacttctcaa
     5761 ccccagcgtc tctctagcct tcttcaagcc attatgccct ggattgttcg tcaggaaaac
     5821 acgggcacaa tcgctacagc cgcacaggga ttccgcagcg cttcaagtac tgcttggttt
     5881 cggtaatcgt aaattgaccg aaatgaaaga tcccggcggc gagaacggca tccgcgcccg
     5941 ctttgagaat ggcatccgcc atgtgctcgg gacagccggc tcccccactg gctaccaccg
     6001 ggatggtgac ggcatcggcc acggcttttg tgatctcaag atcgtagccc ttctgcgtac
     6061 cgtcggcatc catcgaagtg agcacaatct cgcccgcccc aagtctttca acttccttgg
     6121 cccaggccac ggcctccaaa ccggtgccca ctcgaccacc gttgatatga acctcccaaa
     6181 actcccggcg atccttaatc acccgttttg ggtcgatgtt taccacgatg cactgactgc
     6241 cgaatctgcg agaagcctga taaacaaagt caggattacg acatgcagcc gaattaatgg
     6301 agaccttatc ggcccccgca ttgaggagac gccgaatatc gtcgagagtc cgaattccac
     6361 cccctaccgt aaggggcata aaaacaacct cggcggtgcg tcgaaccacg tcgagaataa
     6421 tgtcgcggcc ttcgtggctg gccgtaatgt cgagaaaaac cagttcgtcg gcgccttcct
     6481 cttcgtaccg tgcggctact tccacaggat cgcccgcatc ccgcagatta acaaatcgcg
     6541 ttcctttaac cacccgacca cgatctacat caaggcaggg gatgattcgt ttggctaaca
     6601 taatctactc ttggcagttg caaacccgcc ggttaaccgg ggcttaagcc catcgggact
     6661 agtatgttgc ttgctaccgg tgaaaccgcc cggttctctg tccatttttg ctcatgtacc
     6721 taaaaatccc attggtgacc gcaagcaggt atgacttctt ctcactgtaa atggcctaac
     6781 agacatggtc aacggatgga cttccgcctt ccactgccaa aatgtgaagt agtgtaaagg
     6841 cgaagttagc caaacatatt ccttaagtcg ccgggcacgt cctaaccctt ttttaacaat
     6901 ctcttaacaa atccttaaca tttctcggtt atcatgctaa aacggtgggc caagatgggt
     6961 gagcacacaa aaatccgatc tgcgtgattt cggcaacgaa cccgcaagat gggtggcttt
     7021 tccaagcgaa acgcataaag aggagacgag gtatggtcac gttgaaaaaa atttgcggcg
     7081 ccttggtgct cggcatcctt atcggatggt ggacaccgtc ggtggcacaa gttcaggttg
     7141 acccgcaact gccggactac aaacccgttc aagggatttc gggcaacctg aagagtgtcg
     7201 gatcagacac catgaacaac ctcatgacgt tgtgggcaga aggcttccgc cgattctacc
     7261 ccaacgtgca gatcgaaatc gaaggcaaag gttcatcgac cgccccacca gctctgattg
     7321 ccggcacggc gcaatttggc ccgatgagtc gacccatgaa gagcgccgag atcgatcaat
     7381 tcgaggcccg ctatgggtat aaaccggtcg ccctcgtcac ggccatcgat atgctggccg
     7441 tttacgtgaa caaggacaac cccttgaaag gtttgacctt acagcaggta gatgccatct
     7501 tctccaagac caggcgaggc ggttatcccc atgacattcg cacctggggg cagcttggtt
     7561 tgaccggtgt gtggagtcag caaccgatca gcctttatgg acgtaattcg gcctcgggaa
     7621 cctatggttt cttcaaggag cacgccctct ttggcggtga ttataaggat gaggtaaaag
     7681 agcagcctgg cagttcctcg gtggtccagg gtgtcgccag tgaccgttac gccattggat
     7741 acagcggtat tggctataaa acggccgacg tccgagcctt gccgttggca cccgatgcta
     7801 actcgccctt cgtaccggct gagcctgatc atgcctacac gggtgattat ccgctggcaa
     7861 gatttctgta cgtttacgtc aattacaagc cggggacaac gctcgatccc ctgcgggccg
     7921 agttcatcaa gttcatcttc agtaagcaag gccaggccga ggttattaaa gacggttact
     7981 tcccggtgcc ggcggttatc gccaagaagt cgctggcctc ggtaggtcta taggccgagt
     8041 gttgttacca gcatacgtct gaatcatctg ggcctactgg tcttgactag gatccgatcg
     8101 ctcccatgac taccgtccat cgacgtaaga cctcatgggc tacaaaattc gtcgatcgcc
     8161 tcgccgggtg gttaatcaca ttggggggaa tcgggacgat ctgcgccgtg gtgggcgttt
     8221 gcgttttcct cgtctgggtc gttctccccc tctttaagcc agcaaggttg atcccgattc
     8281 actttgtcgc ccagagcgcg aacgaatcga gcggcggctc ggtggctcca tccggtaacc
     8341 aaaccgtccc attcgacgca aaattattgg ccgtgggtct ggatgaaacc atgacgatca
     8401 tctggtctgt cgatgaggac ggcaatttaa tcgtgcagca atttgcggat caggatgtcc
     8461 tcttcaaaaa agacctttct tctcacggta ggcctcgaac tttagcggtg gacacgcgct
     8521 cgggttatat cgcattcggc tatgaccagg gtcgaatcca attcacacgt gtccatgttg
     8581 cggtgacccc cgcacagata gaggatgttc ccatcgacat ccgcgagcaa gtgacgggga
     8641 aacctccata caaagccctc agcggggacg gcgcatgctg gtttgcgcag ttcggcaaca
     8701 tctatcgctt ggaggttcgt gtcgaggaag agcccgacgt caccgtgggc gaggccgccg
     8761 tggaccggct tgccttttcc ctccgggccg aggtgcgcaa cattgcagct ctgacggcag
     8821 acgggattct gcacatcggc cggaccgata agaaagtcaa ctttttgacc ggcgaggaaa
     8881 gcgttcggtt taccggtggc gcgttgaacc ttcaagatgc gggcatttcc gcgatccccg
     8941 acttccttca gatttccggc gtggcagact ccacctatct gatctggcgc aacggacgta
     9001 ccttgcgtct cgatacacga gaccttacca acccacggat tgccgaagag gtctcgttgc
     9061 tttcgccagg agaaggcgaa atcagtgcgg tatcattcct catcgggaaa atgacgctca
     9121 ttgtgggcag caccacgggg acggtatcag catggtttcc cattaaaccc gcgcacgcgg
     9181 agaccgcgga tggcgtccac cttgtccgag cacacacgta ccggccgggc caaggagcgg
     9241 tggtcgcttt gggcgtttct caccgaagtc gtcttttcgc ggcccttttt gccgacggaa
     9301 cggttcgggt ttatcacgcg accactgaac aagtggtgac ggagggatgc ataagtgaag
     9361 tcgtcccggt cgcagggcag gagcttcgtg atcttccggc tacgggaatc gctctcgccc
     9421 ctaaagatga cggcgttttc gcggtgcttg gagggcaccc aagtctttgg aggttagtcg
     9481 cgccccatgc cggtgtcacc tggaagtctg tcttcggaaa ggtctggtac gagggctatg
     9541 agaagccggc ctttgtttgg caatcttccg gcgggaccga cgattttgaa cccaaatata
     9601 gccttgtccc cctcattttt gggacactga aggcaaccgt cttcgccatg ctcttcgcag
     9661 tccccattgc ctttctcgca gctatttaca caagtgagat tctgccgcgt gaagtccgca
     9721 gcgttgtgaa acctaccatc gaactgatgg ccggattgcc aagtgttgtg ctcggttttt
     9781 tggcagcgtt ggtgtttgcc ccatgggtcg agtcacgatt gctccaagtg ctgtctttgt
     9841 tttacgtcat cccgttcaca ctcctcttgg ggggacattt gaccaatctt tgtcctcaca
     9901 attggcaaca gatcgtccgc cgttatcgcg cttggtttat tgtcttggcg gcgggggtgg
     9961 gggttattct ggcccaatgg gttggcccca ccgcggagac ctggcttttc tccggtgaca
    10021 tcaaggcttg gctggatggt cgcacgggcc gcggcgagcc aggctgggtt ctccccctgc
    10081 tccccttgac gtcactcgcc gtcggatgga tggtgctccg ggtggtcgag cccatgatcg
    10141 agggtcgtcg tccggcgatc tctcgcacgg cttgggccgc actgaaactg gctcttttca
    10201 tagtgggggc agtcgtagcc gtcgcgctag cgtgggcaat cggctggcta ttggtttccg
    10261 gaatagggct tgatccgcga ggcctatttt tagggacata tattcagcga aatgccttta
    10321 ttgttggctt cgtgatgggg tttgccgtta tccccatcat ctacacgatt gcggacgacg
    10381 cccttaccgc tgttcccgat cacctccgcg ccgcttcgct tggggcaggg gccacacctt
    10441 ggcaaacggc gatccgcatt gtcattccaa cggccatgag tgggctgttt tccgccttca
    10501 tgattggtct gggacgggca gccggcgaaa cgatgattgt gctcatggcc gcgggcaaca
    10561 ctcccatcat ggattggaac atcttcaacg gttttcgaac gctttccgcc aatatcgccg
    10621 tggaattacc cgaggccgtg caatacagca cccattatcg catgttgttt ctcgcggccc
    10681 tttgcctttt tgtcatgacc ttcgtgatca acacactggc cgaattggta aggctgaaat
    10741 tccgcaagaa ggcctatcaa ctctgaaaag attatgatct tcgagatttg gagtatgctt
    10801 aagcatgcac ggtttttgat tgtccggtga gccaatcatg gcgcagagtg tcgtcgtatc
    10861 cttaaaggaa aagatcaata accagtggcg cgatcgccct aggtcgcaag gaagcctgct
    10921 ttctgcctac ggggaaccag cgatttggct gaccggggga gccctggtga tctgcctggc
    10981 gatgattctc ggcctgctac ttctcattct gattaatggg ctgaggacct tttggccggc
    11041 acgggtggtg cacattaaac ttcggaatgg tcaggccgtg cttggagaaa tcacccgcag
    11101 cgaatggtat cgcccggatc ccgctttctt ggagcagcta tcgccagatc aacgtgcgct
    11161 gtacgtcgag gcgatagagg cacacgacgg gtgggttaaa cgggaactga tacggattgg
    11221 gaattacgag cttacgaacg agcattttcg ctgggtggcg ctgcccgaaa ttgcaccagg
    11281 cggtggaact tttcccgact gggccttagt catcgagcgc gtcacttggg gtcgctttta
    11341 cggatttcct gtggggtttg ttgttggcga gactgtgacg gccacggaac cccagcaaat
    11401 ttggcaagag tttcagaaac atcatccgcg ggtccgtcag cgatggcgac gcatccgcca
    11461 attggaaacc cgcacactgg gcgatatcaa ttacgccctc gaaaaatcac ggctgcggct
    11521 acgaaccgtc gagctgcggg aagggaaaag ctcccctgcc tatcaggaac tagcccaaca
    11581 atttgcccgg caagaagcgg agttgcaaca agagtacgat tccgtgcgga ccgagattga
    11641 gagccttcgc cgagagaata gccaatacgg cttacggttg cggaccgccg atggccaaga
    11701 aaaagacatc ggcctggcgg acattgtgcg tgcgtatccg gccaatcagt tgagtttggg
    11761 ggagcgacta gccctttacg gctctcgcgt gtgggagttt ctttccgatg aaccgcgaga
    11821 ggctaacagc gagggaggaa ttttcccggc tatcttcggc acggcctttt tgaccctcct
    11881 catgagtctg gcggttgtgc ctctcggtgt ggtggcggca ctgtacctga gggaatacgc
    11941 caaagccgga gtggtggtga gcacagtccg ggtggccgtt aataatctgg ccggggtccc
    12001 cagcattgtc tttggcgtat tcggcttggg atttttttgc tatgttgtag gcggcaccat
    12061 cgaccaggtc tttttcagcg cacggctacc caatccaacg tttgggacgg gtggcatcct
    12121 ttgggcatcc ctgacgttgg ccttattgac aatgccggtc gtcatcgtgg ccaccgagga
    12181 ggcccttgcg gctgttccgc gatccatgcg ggagggctct tacgcctgcg gtgcgagccg
    12241 gtggcagact atccgccgga tcgttctccc acgggccatg cccggagttc ttaccggggc
    12301 cattttggcc atggcgcgag gggcaggcga ggtagcccca cttatgctcg tgggcgccgt
    12361 caaattggca cctgagctcc ctctcgatca tcggtttccc tatctccact tggaacgcag
    12421 tttcatgcac cttggctttc acatctacga cttgggtttc caaagcccca acagcgaggc
    12481 cgccaagccg atggtcttct ctgccacact gctactcatt ttgcttgtag ccgctctcaa
    12541 cttgggggcc atggtcttgc gctcacggct gcgaaaagaa atgagggcca cgcatctttg
    12601 attggcagtc gaggaatggg ttataatgaa gggtcgcgtc ttgatgaacg gcgaagaaaa
    12661 gcggacggca aacggccccc ttacccgctt gatcgcggat ggaccggccg tgacccaccg
    12721 aaaacggcgt cgggagactc ttatgccaga ccagtggccc gatgagagtc cgggtacagg
    12781 caaactcata cagacggaaa cagaaacagc ctcccctgtg gctttaggga cgcctgttcc
    12841 gttggcaaag gtcctctcgg ccggtgcttc ccggttggat gccattgccc gcggcgaacc
    12901 ctttgaaagc gagattcatg ccgaattcgc ccaagaaacg ccgattttag agattgatca
    12961 ctttagcctc tcctacggcc cgaagcaggt cctcttcaac atctcgatga tcattccgcg
    13021 tggaaaggtc accgcaatca ttggtcccag cggctgtgga aaatcgacgc tcattcgctg
    13081 tattaaccga atgaacgatc tgatcgagtc ggtacgcatc gagggggata tccggctgga
    13141 tgggacttcg gtcttcgacc ccgcggtgga tgtgaccgag attcgcaaac gggcagggat
    13201 ggtgttccaa aaaccgaacc cgttcccgat gagtattttc gaaaatgtcg tctatccgtt
    13261 acgcatccaa ggtgttcggg atcgaagagt tttggcagaa gtctgcgaac ggagtctgcg
    13321 ggcagccgcc ctttgggacg aggtcaagga caacctccac caaaacgcaa tgcaactgtc
    13381 cggaggacag cagcagcgat tgtgtattgc gcgggccctg gccggagatc cggaggtcct
    13441 tctcctcgat gaaccgtgct ctgcgcttga cccgattgca acgaataaga tcgaagacct
    13501 cattcgggaa ctggcaggga cttatactat cgtcattgtg acccacaaca tgcaacaggc
    13561 ggcacggatc agcgacttca ccgcccttct ctatctcgga cggctcattg agtacggccc
    13621 aacgcgccgg atattcagtg ccccgcatct gcctgaaacc gaggcttacg tcaccggacg
    13681 ttttggctaa gcgggctttg ttttcaagaa tgcggggacc atgtaaaatc gagtggagaa
    13741 gtgcacggaa aataacccat tcaggagttg actatatgac gttatccggg atcgatctat
    13801 tgtcgttcgt ggtgctggcg caagagggta ccagcgcaac ttcgtgggca ccaggctatc
    13861 tggttacgat tctagtgctt ttgttcgtca tgatggtcac gctggctccc agtcggcggg
    13921 ctgacaagcc caaacaattc gagttcacgg gaacatcctg gggcaaggtg gtgaccggcg
    13981 agacggagaa gaagcaggag ccccaagggc ctcccccggg ggtttttgct ccgggacaat
    14041 tggcacaaaa gcggaaataa acttccggtt ccggggtgga atcccaggtg tgaaaacctc
    14101 tggttatccc gatcgggtgt ttgttcgaga atggacgagg gttttcagtc tgcttcgcct
    14161 ttggaaactg aagcttgtcg catcccgcgc agcgtggccg ggtgtttcag gaagtacggc
    14221 tggccgacct gaatccgcgg cggatggctt ttaaagaact ccgcaatggt ggcgacaacg
    14281 gtgcgttgtt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgccggcgg
    14401 ccaggaagga cctgtggcag aatgacctgt gccagtttgt attcggcaaa gagctgataa
    14461 tagcgatcgg caatctgttg tcgttcggca atccaggttt ccaggcaatt gaacttaacc
    14521 aagagcgtgg ccgcgtgaaa agcatccatg cggctgttga tgccaatcca gtgatgataa
    14581 tatcgtggtt tcatgccatg gacccgtaag acccgcagct tttcggcaag ctcggcatcg
    14641 tgcgtggtga tcattccggc gtcgcccatg gctcccaggt ttttcgtagg ataaaagcta
    14701 aaacagccga tctggcccaa agatcccaca cgccgttgat ggtactcagc cccaatcgac
    14761 tgtgccgcgt cctcaatgac aaagatgtga tgctgttggg ccagcctcaa aatgggttcc
    14821 atttccgcag cttggccaaa aaggtgaaca ggaataatcg ccttggtgtt tttatggatc
    14881 agtcgggcaa cttctcgggg agagatgttg taggttgccg gatcgatatc cgcgaacacg
    14941 ggttttgcgc ccagtcgggt gaccgcactc gctgtggcaa agaacgtaaa actgggaacg
    15001 atcacctcgt cgcctggccc aattcccaag gccatgaggg caagcagcag ggcatcgctc
    15061 cccgaggcac aggcaatagc gtgctcgcag tggcaatatt gggcgaggat ccgctccagg
    15121 tgttcgacct caggtcccaa cacaaaggcc ccggaatcgc aaacccgtgc caaggcctcg
    15181 acgatctgat ctcgcaaggg tctgtgactt cgagcaagat ccaagaatgg aaccggaatg
    15241 cgatgagatt cgctttcgaa actcggtgtc gccgctccct gctctggctg cataaaagga
    15301 cccttccttg gttgccggca caatttctgc tggatgatca gtttagtgcg ttcgctttag
    15361 cgtagagttt tggagtcgcg ttcgtcaaga caaatttttt acccaaagtg gagaggttcc
    15421 ccctaaggcc gtccggcttc actgctgctc agccgaacaa ggaaaggaca ttacacataa
    15481 tagcccggga tgctcagacg atgataagtc tatcagatgg caatagacca cctttatagg
    15541 ggatcgcgcc gcccatgcaa caacgatctt ttgtgaacat cttcgggttg atcttcctac
    15601 cctgggcgat tggctgtctt ctggcatcag tggttggcta cgtcggtctt acgtggcagg
    15661 agcgatcact gaaacaggcc caggccgagg agtgggagcg atttataagc ggtctcatcg
    15721 aaggggagat cggctcgcag cccaaaatca ctgacttaca agagagcagg ctcttgcaat
    15781 ccctgattca gaacacccgt ttggagctcg cggtgttcac ggcctcggga aaatatgtgg
    15841 ttgggagcgt gaccaatcca ccgcccaaca tcccgtcaga agttcaaatg gtcttaaaaa
    15901 ccagccagcg gttcaccacc tggacagaga aacgcgggca aggtcgccct cagttgcgca
    15961 tggcgtggcc aagacaggtc gagggtaaga cacacatcat ttgggcggcc attgacctcg
    16021 gaacggtgac cggcaatggt ccttccctcc gcggtctctt ggcgtggaat gttgtctttt
    16081 ttgcgatagg gctccccttt gcgtgggggc tgacatggtg gctgaggcga cggttggacg
    16141 tcgttacaaa atccgcccta gaaggcgaac cttccctccg cccatgggac ttatgggagt
    16201 ggcacgccct tgcccaccgc ctccgtgact atgtggcaca gcagcgccag cagatcgagg
    16261 ttttgcaagg tattgcacga gaccatcaag cagtcctgga acatctccca gatggtctcc
    16321 ttttcgtcac gccggcaggt cgtgtcgcta agcacaatcc ggcagcaggc caactgttgc
    16381 gatttcccgg tactctggcc gagggtctat ctatccgaga atgcttgcgt aaaccggagt
    16441 tcctgacctg ggtcgaggat ctcttgtcgc taaagcgccc ccctgtgctg tcgcttcaca
    16501 atgacgatcc cgagcaacac ttggaaatcc ggggagctga acttaaaggc cctgaccagc
    16561 gcgttcacgg cttccttttg atcattcgcg acatgacacg gacttgggca gcggaccgtg
    16621 tgcggcggga ctttgtggcc aacgtgagcc acgagctacg caccccgctg acctccatca
    16681 aagggtttct cgagacacta ctggacggcg cccttgagga tccaccccaa gccaatcgat
    16741 tcgtaaggat catccacgag cagaccgaac gtcttgaacg gatcgtcaat gatcttttga
    16801 ttctcgcccg actagaaggt gaacacgagc aacccatcac aagggaacct gtccgcgttg
    16861 aggaactcgt tctgggtgcg gtcgatgcct gccggtacca ggccgaacag cgcggcatgc
    16921 aactccactg ggaggtcccc aaggacttaa ccatccgtgt gaacggtcgc ttatttcatc
    16981 aggccctggt caaccttctc gataacgcca tcaaatacag cgatccgaat aaagaaatcc
    17041 gcattctcgt ccgccccggc gaaaaggagc ttatctttga ggttcaagac caaggttggg
    17101 gtatcgaagc ccgacacctg ccgagaatct ttgagaggtt ctatcgcgtg gatccaagcc
    17161 ggagtcgcca attgggaggc actggcttag gactggccat cgtcaaacac gttgcccaaa
    17221 ttcacggggg gcgtgtcacg gttgacagcc gtgtcggtgt gggcaccacc ttccgcatcc
    17281 acttgcctgt cgagcacgtc ctccacgaaa ggcctacaga ccgacaatca gaatgaagcg
    17341 agctgggtca cgatggcgac tcggttccct ccgattcggc cccaggcggt tctactattt
    17401 cgtcctcttc tttacgattg ctacgggttt cccaccaacc cgctaaagtg acgccgcaaa
    17461 agagaacgat cgcaataatt ccaacaaccg ccacaatatc ccgcagcccc agccaaatgt
    17521 tgtggtcgcg gacgtatctc gcttccatct ccagccacgg agaccgctca cccgtgcgca
    17581 agaccttaaa atactgatac gccgcggcgg gcgggagttt gtgaaagtcc ggcagccgtg
    17641 ggcgatagat atacagcaca atcagcccga taagtgcaag cacggccagg gagccgctta
    17701 tccactgaaa tagtcgaacc caccctgaaa gaggggcttt ttcctcttca actgaggcgt
    17761 cctcatcaat ggtctgtcgc gtaccaagca attctcggcg cgaagggacc gcaagttcgc
    17821 ggccacatga acaggtgacg atcattccgc actggttgat tgtcacgggt accgatttgc
    17881 cgcatgggca ggggatttca atctttgcta ccataagaac aacctgcgag tgtttgacca
    17941 cgcatggccc ggctggaaga ccaccctacc ttttttagca taaattgctg tcggacgctg
    18001 tacctgagat acagatcggc gtaaagttaa tcatagagag cggcgtccta tggcatagca
    18061 aggcagcact tacgtctgtt aaaatttttc catatcggtt ctcgaaggcc ttgaactcct
    18121 ttgtcaacca ctgtttcatg cctcgattgc gaaaaccgca cgataactta acgttggtta
    18181 ttacgtagga cgaaccgggt tggccttttc cccgatgtcg gcaacatctg aggcaggggg
    18241 taacccatga agaccgtggg ctcctcggcg gagggagtaa cgcatcatgg acaacctgct
    18301 tggtgataac ctgcgacgga tcatgtctca actcggccta actatcaatg aggtcgccga
    18361 acggacaaaa ctcgataagc ggacaattcg gggcattctg gaagggagta agcgacctca
    18421 tcccaagacg attggccaac tcgccgaagg attgggggtc tcggtcgatg agctgtacat
    18481 cgatccgact cgcctcctct atcgaaggtt agataagcag accaaccccg ttgtggaaaa
    18541 agtgatccag gaatatcccg agttgttcgc cgactggacg acagcggaat tcgaagagct
    18601 ctatagccgc tttgggatgg gcggtgccat gaccgaggag ggggtcctgg aagcggcccg
    18661 aaagaccaac cgacgccgca tcctgcaaca caaactggcc gtcttactgg aaaccagtca
    18721 ggccgagctg atcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttgg
    18841 gcatcccgtc atgcggactg tgaaccgttc cgcatccaac ccaagctttg caacttcacg
    18901 ttccaggtca tcgatcagtt tgggcaatac ccgttcgctt tcggtgacag ccaacggaca
    18961 ggtgggaagg gccacgcacg ccattgacca gcggcgaacg ttggaaattt cctcactcaa
    19021 aggaacaccg taacgccgca agatctgttc aatctccgat cgggccgagg cctccaaatc
    19081 gcaaaagagg acgctttgct gcggcgtgaa tcgaatactt gggtggagtc gttggcaaat
    19141 ctcccggagg gcacttttca aacggaaggc gtcccgatcc acgatacgcc cattttcgag
    19201 attgaggcca taaaagaatc gcccatcacc ttgctcatgc caacccatat ggtcatcaac
    19261 ctcggtgaca ccgagtggta atgggtcggg caaagaccga cctaggtagc gctccgtcat
    19321 ctctcggaac caaggtagcc cgcggtccgc aatgaggtac ttgagtctcg cccgcttccg
    19381 gtcgcctcgc tcaccataat cgcgataaat tttgacgacc acttccgcca ccggaaccac
    19441 ctccgcaggc cggacaaaca ccaggggctg actcaaggcc gggaaggtat ttttgttact
    19501 cggcgttacc cccaagccac cgcctaccag cacattgaaa ccaactacct cgccgttttc
    19561 gacaacggct agaaaaccga gatcgttagt gtaaagatca acgcagttat cgtcgggtag
    19621 cccaaaggcg atcttgaact ttcgcgggag ataggttttc ccatagatcg gctcaacctc
    19681 cgcaacctct cctgacggct cggtgacttg ctccttttca cccgtatccg ggtctgtaag
    19741 ccaaatctca tgataggccg gcgtccttgg cagcaggtgg cgagtaatct cctgggccaa
    19801 gttttgaagt tgctcccgaa cacggttgtg ccgcaatggc gcggggcagc acatcacgtt
    19861 ccgaaccacg tcgccacagg caccgagcgt ggttagctgc acctcattga tccggcggat
    19921 agtttctcgc aagttcccct tgagaacgcc gtgcaattga aggtcctgcc ggtcagtaat
    19981 gcgtgccgtg ccgttaccta gctggtcaca aaggtcgagt tgagtaagaa actgttccgc
    20041 cgtcagtaca ccacccggga ccttcacccg aatcatggtg aaatacacct ttcccccgcc
    20101 cgaggccctt tgaagctttc gggcatcacg atcgtcctgc tggtacgagc cgtggaactt
    20161 aagaatctgg gcagccgact cggaaaagct gggctggtcg ttgttcacct cctccgcaag
    20221 gggatgccga aggtactgac tttcctcttt cagcttttcg acctgacttc gcttgactgg
    20281 ttgggcttcg tggtccatgc gtgcatcgtt cctttctcgt cgtatccgga gagacaaatt
    20341 tacatcatta tgttgatcac attactgaca cattaaattc tacagccaca attgacgcca
    20401 gaaaagacct ggcaacggtt ttttgcagaa aaaggccgat agcgaaatta cgccctgaga
    20461 tgagaaaacg cggcacgagg aggaagtaac ctcacttctc cagcgtcaca ttccagtagg
    20521 cctcgctgac gaatcgtgtc cacgtctcga tgcgaatctc ttttgcggcg taaacgggac
    20581 gccatttggg gcgggtgggg cggcgtttca atctcatgcc cgcctcctcg ggggtacgat
    20641 tccccttccg acggttgcaa tccacacagg caaggacgca gttttcccag gtggattccc
    20701 ctcccctggc ccgcgggatt acatgatcga tcgtgagctg atcgggaggg aattgccgtc
    20761 cgcagtattg acaggtgtta tgatcccgtt tgaagagatt ccttcgactg aacggaactc
    20821 cggaataagg aacccggtcg aactcccgca aagtaatgac ttctggtacc ctcagtcgga
    20881 aggaaacccc ttgaatgaag ctttctccat ccgccggtgc caactgtgcc caatctgacc
    20941 agctataggt gctaaagtct tctggatcga cgaccaaggc cgtcccttga tagaccatga
    21001 ctaaggcccg ggcaaccgtc gccacgtgga tgggttgcca attccgattg agcaccagtg
    21061 tgggccgctg aagtacacca ctcgccacag ccgcacctat gaggaagcgg tcccgcaggc
    21121 agcgtggatg gggacagtct gagcagcact taatgcccaa acacccatcc gctacctgaa
    21181 gacggaaggc agcgttcacc cggccaaacc cacacgatcg gcctcccgga ggcgttcgct
    21241 gatccgattc cgccttggac cacactggtt ttagtttacg aattgataag gaaaaaaaca
    21301 acaatcccgc cttttctccg gggaaaccgg acggtgaatt cttacgacac ggtcacggca
    21361 gatgattttt gtcctgccac tcctagaggc acgccccaat gtcccctcgt tcttgttcaa
    21421 agctgtggcc cagtgatata gtgatatagt gagcgtgaac gacgtctcgg agcttccttc
    21481 cgctatccaa ccgtcgccgt agcaaaggag gctgcctcct gcggaaaagt actgcgaaca
    21541 acaagggttg agcaatttat ggctggtcat tcacactggg caagtatcaa acacaagaag
    21601 gccgccgttg atgccaaacg cggcaagatc tggagcaagg tctccaaggc gatcatcata
    21661 gccgcccgca ttggcggtgg tgatcccaag atgaatcccc gccttcgtgt cgccatcgag
    21721 gaagccaagg ctgtcgggat gcccaaagat aacatcatgc gcgccattaa gcggggcacg
    21781 ggcgaaattg agggagcggc gcttgaagaa gtcacttacg agggctacgg gccgggcggc
    21841 gtcgccatca tgtgcgacgt gctcaccgac aatcgtaacc gcacggcggc ggaaatccgc
    21901 aagtgctttg aactccacaa cggtaagttg ggtggagctg gctgtgtggc gtggatgttt
    21961 gaaaccaaag gtttgttcct catccccgca gatcaagtga atgaggaaca actcatggat
    22021 gttgccttgg aagcgggtgc cgaagacatc aaacaggtcg gtgacaagtt tgaagtcacg
    22081 tgcgatccag cgaagttctc cgatgtcagt gaagcgctcg accgggccaa tattccgtgt
    22141 gattcacgct ccgtgacccg tatccccaag gactatgtgg atttggacgt cgagacagcc
    22201 cgcaaagttt tgaaactcat ggaagaactc gacgaacacg aggatgtgca gaatgtatcg
    22261 tcgaatttca atattcccga cgaggttatg gcggagctat cgcgctcgta gtcgaggcaa
    22321 cgcaccaagg gtggcaccga tctaaaggag gatcccgtgt caaagatcgt tcgtttctcg
    22381 atttcgctgg agcgtgatct gttaaagaag tttcagcgtt tttgccggga agagcggttc
    22441 gcgactcgga gcgaggccat ccgccagttg atccacgact tccttgcgcg aagggcctgg
    22501 gttgaagatg cggaagacgt cgcggggacg ttgacgttgg tctatgacca tcaccggtcg
    22561 caactccgcg accaattgat gaaacttcag cacgatcata ccgacctgat cgtgtcaact
    22621 cttcacgctc acttgacggc ccacctgtgt ctcgaggtca ttattctgcg aggacctgcc
    22681 aaacaattgc gagagattgc tgcacggttg cgaggtctca agggggttca caaaggcgag
    22741 ttggtcatcg cggccgtccc ccaggaatca cctgcctgaa cgtttgaatg ctcggatttg
    22801 gccgcgtacg gcgctggagc caggtttagt tcctccgccg ggcggcatgc ggtcttgacg
    22861 attgctctgg caatgggcaa agtggattga tccgtggctt gtatgggcaa aacgcgggcc
    22921 aaggtgggga gtcaaacaac gttccggtcg atgaactgac tgaatcctga ggtgcgacca
    22981 tgctgaaagg aaatgccatc gtcggtcaaa gtgggggtcc aacggccgtc atcaatgcca
    23041 gtttgtgcgg cgtgatcgag ggttgccgcg agtcttccca gatcgaacgt tgcctgggaa
    23101 tgcggtttgg catccaaggc tttctggaaa gcgacgtcat cgacctcgat cgggaatcag
    23161 cggaaacgat cagccgcctg agaacgaccc caagttccgc tctgggaagt tgccggtaca
    23221 agctgcggga cgaggatttt ccacgacttt tggaacaact taagcggtac aacatccggt
    23281 acttcttcct gattggcggt aacgacacca tggacacgat tcaccgcctg gaggaattct
    23341 gccggaaagc gaattacccc atgttgggtg tcggcatccc gaagacggtg gataatgacc
    23401 tcttcggcac cgatcatacg ccaggttatg gcagtgccgc ccgctatgtg gccatttctg
    23461 ttcgacaggc aggaatgctt gcccgtgaca tgaagcgcgt ggaccagttc gtgatcttcc
    23521 aaacggtggg gcgctccgcg gggtggttag cggcggcttc tgctctggcc cggtccgccg
    23581 aagatgatgc cccccacctg atcctcttgc ccgaacgtcc cttcgaccgg gacaggttcc
    23641 tggcggccgt caagcagacc tatgacaagt acggctggtg tagcatcgtc tgcggagaag
    23701 gaatcacata tcccgacggc acccccgtca gtgcggcccg ggtgaccgac cagttttcca
    23761 acatcgaatt tggggccatg gggggaacaa gtgccgccat gatgcttcac cgcatgataa
    23821 gtcaggcatt cggctggcgc ggagagttcc aggtctgcga atcacttcag atgtgcgcag
    23881 ccgatcgcgt cagcgacatt gaccttgaag aggcgtatgc gtgcggtcgg gaagccgtcc
    23941 ggctggcaaa tgagggtgtc agcggcatga tggtctccat tcaacgggag tcttcctcac
    24001 cctaccgcta tcgtttagga gtcgtgccac tggctgaggt agccatcaag gccaaaccca
    24061 tgcccgatga attttattga taaggaaagg ctctatgtca cggatgcctt tgtggagtat
    24121 gcccggccat tggtgggacc tttgccggaa tatgtccggc tcgcaggtta tcctgtgaat
    24181 tagggcgcca ggcaggcaga gacacaatct tcggaagatg ataccagact tacgaggctg
    24241 acaatggaac tacagctcat tcggaaggaa caggcacagc cccccttctt tgtgggagtc
    24301 gatttgggtg gaaccaacat caaagccggc gtaacggatg atcgaggtgc cgtactttgg
    24361 gtcgggcagg agcctacgca cgttgaaaga ggcccggagg acggcgcacg gcggatcgcc
    24421 gatcttatcc accgagctat ccacgaggct ggactaacgc cccaagatat cgctggggta
    24481 ggtctgggct ccgctggaac catggacatc ccgggcggcc gactcgtggt ccctcccaat
    24541 ctcaaaggtt gggaatactt tcccattcgt gaccgcgtta gtgaacatac cggaatgccc
    24601 atcaccttcg ccaacgacgc cacggcggcg gctttcggtg aaaaatgggt aggggcaggt
    24661 catcggttcc atagcatgat cttgctgacc ttaggaacgg gcatcggttg cggcatcatc
    24721 gtcggggacc tgctcctgga tggtgaacac agtcacgggg gcgaatacgg ccacacggtg
    24781 atcgactgcc ggccggacgc gcgggtttgc tcatgcggac gacgtggcca ttttgaggcc
    24841 tacgccagcg ccactgccgt caccaagcga gcggaggagg ccctggccca agggcgggtc
    24901 agtatgttaa cacgtcgcat tcaggaaggt gcaaagcttg acccgaaact aatcgcagaa
    24961 atggccgagg ccggagacga actctctcgg caattgatcc tcgaaaccgc ggaataccta
    25021 ggcattggca ttgtcaattt gatgcacacc atcgatccca acggtatcct cattggtggc
    25081 gcgatgacat tcggcggcaa ggagagccgc atcggccgta tcttcctgga acgagtcaaa
    25141 cacgaaattt tcctgcgagc ttatcccgct gtgggagaca caacggaaat cgactttgcc
    25201 accctcggtg gaaacgcggg ctttatcgga gccgcgggga tcgcccgcct cgaaaaaatg
    25261 aaagcccaat tcccacccgc gaagtaaggg tgcaataaca cagtgtcaca acacatggcg
    25321 aaacctatga ccatcgatct tgttcgtcaa gaaatcgcca cagaggcccg acttatcgtt
    25381 gtcaaggttg gaacccgcgt tgttactcac cctgacggcc gactgaacta cgaacgcatc
    25441 gaacagttgg ccgaggaaat ccactgtgtc cgccaaacag gtcgaaaggt cgtcctggtc
    25501 agctccggtg ccgtgggtgc cggggtgggc cgattggggc tgccggggcg cccgacggat
    25561 ttgtcggaac tccaagctgt tgctgccatc ggtcaaagcc tcctcgtcga ggcctacgac
    25621 caaaccttcc gccgctacaa cgacgtggct gcccaagtcc ttctgacggc cgatgtcatc
    25681 gaaaatcgaa cgagctacct caacgcgcga aacacccttc tggccttgct caaccggttc
    25741 gacgccgtgc ctatcatcaa tgagaacgac acggtcgccg tcgaagaact tcgcatgaca
    25801 tttggtgata acgatcgcct ggcggcactt gtcaccaatt tgcttcaggc tcaactcctc
    25861 gtgttacttt cggatgtgga aggtctttac tccggcgatc cgaatgatcc caaatcggag
    25921 ctgatccctc tcgttcgcaa gattgacgat tccatctttg atctggtgcg ggacaaaaag
    25981 accggtttga gcaaaggtgg aatggccagc aaactgaaag cggctcaact ggtcacaacg
    26041 ggtggaggta acgtgattat tgcctccggt aagacgccgg gagtcctgaa gaagatcatc
    26101 gctggggagg ttgtcggcac cttgttcttc gccaaaggtc ccactgtggc cgcccgaaaa
    26161 cgctggttcg gccttagcat ccaaccgcgc ggacagttga tcgtcgatgc cggtgcccgg
    26221 gaggccattg aaaaacgcgg caaaagcctt ctcccgatcg gaatcctcga tgtcgtggga
    26281 caatttcaaa aaggtgatct ggtcgctctg cgcgacccag acggctgtga attcgggaag
    26341 ggattgtcca actattccgc ggaggacgtc cgccgcatca aggggctcag gactcgacaa
    26401 attgcccagg tcctcggctg gctgccgtac gaagaagtga tccaccgcga taatcttctc
    26461 gtcacccggc cttgatcgcg ccggcggatg atccacgatg atgagccgtt ttcacgacca
    26521 cgttgccaga aaaagcggga ccggcgcctt gtgacaagag gcggggttgg caacgctggt
    26581 atcgttgttg tgtcattggt taaaataaca ttggtaggtt tgactatgcc catgatcaac
    26641 gagccaccac cactgaccta cccaaggtgt ttcggcggcg aaaagaaaac accccgggca
    26701 gcgtcataag gtgaatgatt tccctgtcct aaaacactgg gtgattgttt ccggaacgga
    26761 gaatttcgag gcacagtgtt atggcaatgg acgacaagtt gccgaattcc catcaaccga
    26821 aaccccctcc cctcggagcc aaggaaaagc ctgcggataa accggagctc gatcccaaga
    26881 cttctgcccc ggtggagagc cagcccagtg gtgctgcggc gcaggaaaag actgtgtctg
    26941 gccgtaaggt aacacggtct cccaaagccg agaattcaca ggccacaaca acgcagggga
    27001 tcagtgcagc agcccagcaa agggaaccgt catccgctca agccgctgtt caaagccagg
    27061 aggaacgtcc ccgctcgcgc gtcacaacgc tgcttgtgga aatccttcac tggatgccaa
    27121 gctggtttgt cagcatggtc gtccatgcgg tggccttgct ggtcatggcc atgatcacaa
    27181 tccccggacc gatgcaagat gccgttcagc aacttgtggt ggcacccggt gaaaatgagc
    27241 agttggaaga gatcgaggaa ctcctcaccg aaccgcccga agatatcaat ctggccacgg
    27301 acctttcgcc tgtggaggtg gccagcacca ttgatagctc acaggacatt tccccgttca
    27361 acgacgaggc ggccgcgatg gcctcgttcg agcttaatcc catcggcttg gaacacgccc
    27421 ctcagaccga tcttttggcg tccatcgggg ctttgtccgg ggacgccttt tccggccgag
    27481 gatcaggcaa ggaacgcatg gttcgcacgg ccggtgggag tgaagggagc gagcgggccg
    27541 tggccttagc gttgaagtgg ctggccgatc atcagatgcc cgacggcggc tggagctttg
    27601 agcaccaaaa agccggtggt tgcaatggcc agtgccgcaa tccagggaat ttgccggatg
    27661 cgcgggctgc ggcgactgcc ctggcccttt tgccgtttct cggtgctggt caaacgcatc
    27721 gcgaagggca gtatgcgaaa aatgtccggg ccggattgta ctacctgatc accaatcgca
    27781 tgaagatctc gcccgaggga ggtagctttc tggaaaaagg cggcaacatg tactcccatg
    27841 gtctggcagc cattgtgctt tgtgaggcct acggaatgac gcacgacaag gtccttgcgg
    27901 ccccggccca gcaggccatc aatttcattt gtaatgcgca ggatccagta ggcggcggtt
    27961 ggcgctatca accgcgacaa aagggagaca cgtcggtcgt cggctggcaa cttatggctc
    28021 tgaaaagtgg tgccatgagt tatcttcaag tccccaaggt gaccattcag aaggcccaag
    28081 cctttctcga ttctgtccaa gccaacgatg gggccaccta cggatatttg gaccccggtg
    28141 ccgatcctgg cacgaccgcc gtcggcctcc tctgtcggat gtaccttggc tgggacaaga
    28201 atcaccccgg actgaaaaaa ggcatcgcac agctcagtgc atgggggcca tcgaccgaca
    28261 aaagcggacc gagtaagaac aacttctact acaactacta cgcaactcag gtcttgcggc
    28321 actgggaagg cgaagaatgg aaaaagtgga acagcgttat gcgcgattat ctcgtcaaca
    28381 cgcaggcgca aaagggtcac gaaacgggaa gctggttctt tgaggactgt tctgatcacg
    28441 agaaagaacg cggtggccgc ctcggtggcc gcctctactt cacggcgatg gcaacgctca
    28501 ttctcgaggt ttactatcgc cacatgcccc tgtatcgcaa acaggcaaca gcagaaaact
    28561 tcccgctcga ttgagagcac ggctcaatca ggtgagcagt aaatcgcttg ccgcctcttg
    28621 acacaccgac gcgctggttg tacacttgta cacaagccag cctggggatt aggttctttc
    28681 aagacgagcg aatgagccat gggaaaaaat gatatcgccg ccctttcgtc attgacgcca
    28741 agacagcgag agattctcga ttttattcgg gattggatcg agcagttcgg ctacgcccca
    28801 tcggtacggg acatcgggcg tcatttcggg atccgctcac ccaatggcgt gcggggacac
    28861 ttgaaggccc tggaagcgaa aggtgtcatc gaacgcgatc cgcggcggtc cagggccttg
    28921 cgtctgacac ctgccgctcg tggcagggtc tcacgatctg gtaaggggtt tacgctgccg
    28981 ctcgtagggc gcattgccgc cggccaactt cacgaagcta ttgaaaatgc cgaaaccttt
    29041 gatttccaaa gccttttcag cagcaagtcg gctgatcatt tcgtgctccg tgttcatggc
    29101 gattccatga tcgaggccca aatcgccgac ggtgactatg tcatcgtgcg acggcaggaa
    29161 acggccacac ccggcagcat cgtcgtggct ttgaccgatg aaaacgaagc cacgctcaag
    29221 tactggtatc ccacaaaacg gggagccaag ctcgtccccg ccaacaagcg gatggccccc
    29281 atcttcgtca aaaacgccaa ggtcctgggg gttgtcgtgg gcgtggtcag aaaacttgcg
    29341 taacacccct tccgccgcat gattgagacg ggtgtcgttc tagcctcgtt atgacgccaa
    29401 ttgcggcgac aacattgtcg cccgtcgtgt gttatcgcac cgcgccgcga tgggacggcc
    29461 gcatcagtta gtatcaatgc ttaaagtgcc gccgtccggt gaagatcatc ggcaacccat
    29521 gcttgttaca ggcggcaatg acctcctcat ctctcacgga ccctccgggc tggatgatcg
    29581 cgcggatgcc ggcctgcgcg gcgtgctcaa tggaatcagg gaaggggaaa aaggcatccg
    29641 aggctaggac gcttccctgg gcccggtctt gcgccttgcg gatggcaatt tctacagagt
    29701 cgacccgact catttgcccc gctcctaccc cgacgagcat tccattccgg gcaaggacaa
    29761 tggcgttcga cttgacaaat cgcaccatgg cccaggcaaa gcgcaggtcg gggagaaggc
    29821 tttcatccgg ccgaacttgt gtcacgacct gccattggct ttcgtcgtcg gctaaattat
    29881 cggcctcctg aacgagaaga ccgcccgtca gatgccgata cgcccatcgt tcgcgttcct
    29941 ccgtcaggtc ccccacctcc agcaggcgga catttttctt ccacttgggt ttgctggtca
    30001 gaatctcaaa ggccttggca tcaaaatgcg gggcaatgat cgcttcaata aacaagccgg
    30061 gttcagaaag gacctcggcc gtttccgcgt ccaccgtgcg attcatcccc aggatcgaac
    30121 caaacgcgct gacggggtcg ccggccagtg cgtttcgtgt ggcctccgcc aacgtggccg
    30181 ccacggccgc cccacaaggg ttgttgtgct tgatcacggc caccgcgggt tcccgaaagt
    30241 ggcgcacaat ctccagggct gaatcaaggt caagcaaatt gttgtaggaa agttcttttc
    30301 catgcaacgg ccgggccgtc accaccgaag gaccatgaac ctcgggcatc cgatacgccg
    30361 cggcttgttg gtgaggattt tctccatagc ggagaggcgt ccaccgctca aatgtgaaaa
    30421 acagccgcga gggaaaaggc ccttcttcca cacgttgggc aaaccaagtg gcaatggcct
    30481 gatcgtattc cgccgtatgg gcgaaagccg ccgccgccaa ctgccgccgc aaagccaagg
    30541 tagtcccgcc gtgttcacgg agttctttaa gcaccagttc atattgggca ggacttgtga
    30601 cgatggccac aaaggcatga ttcttggcag ccgcacggac aagggatggt cccccgatgt
    30661 caatgttttc aatggcatcc gcctctgtgc agttggggcg ggcaactgtt tgccgaaacg
    30721 gatagagatt caccaccacc acgtcgaacg tcaagatatg atgctcctcc agtgcccgca
    30781 catcatccgc atgatcccgc cgtgcaagga ttcctccaaa gaccttggga tggagcgtct
    30841 tgacgcgccc ctccatcatc tcgggaaagc ccgtgtaagc ggccacatcc gtgacaggga
    30901 tcccttgctc ttgaagaaag cgccgtgtac cacctgtgct aaagatttca agcccaaaat
    30961 cgcaaaggcc ccgcgcgaaa tcgcccagac ctgacttgtc gctcacgcta atcaacgccc
    31021 gccgcggttt gacaatcatg gacattcact ccagacgcga tgaaaacacg acccaatcaa
    31081 gcgagatggt aaaagcctta ttttaaggtc tattggccga aggtggaacg aagg
//
LOCUS       DAOSUT010000029        30408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1021_length_30408_cov_9.273647, whole genome shotgun sequence.
ACCESSION   DAOSUT010000029 DAOSUT010000000
VERSION     DAOSUT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 30408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30408
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_30408_cov_9.273647"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..649
                     /locus_tag="PLD05_04220"
     CDS             338..649
                     /locus_tag="PLD05_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76669.1"
                     /translation="MTRKKYTIRVVTRGADGEIRIRDYDSPEDLLKRHTQVGIDDCCT
                     DLSVRGLPVVKGLIGPIPEAPGIVRYESPEVFETLTKEWSMTKITRRRTRTQRPDLNV
                     K"
     gene            complement(779..2041)
                     /gene="lysA"
                     /locus_tag="PLD05_04225"
     CDS             complement(779..2041)
                     /gene="lysA"
                     /locus_tag="PLD05_04225"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOP76670.1"
                     /translation="MAERKTLRCDIAGIDVVSLAEQFGTPLYVYDAETIRLRFNELRK
                     FDVVRYAQKACSNLAILSLLRQLGALVDAVSAGEIHRALVAGYAAVGDPPPIVYTADI
                     FDRDSLELVVEKGVHVNCGSRDMIQQLGERAPGREITLRINPGFGHGHSRKTNTGGEF
                     SKHGIWFKELGEALAQAKELGLSVTGLHMHIGSGSDFEHLAKVCSAMEEVAFAVGPSV
                     RSISAGGGLPTPYRDSDQRIDLERYFELWNTTRRKLESAFGHPVRLEIEPGRYLVAEA
                     GYLVTEIRAVKKQADNLFYLVDAGFNNLARPVMYGAYHPISVVPRDRNFARPEYDVIV
                     GGPLCESGDIFTQEEGGVVVTRRLPQASVGDYLVIECAGAYGFVMGSNYNAKPLCAEV
                     LIDGGRPQLIRRRQSYRELWEGESIPQG"
     gene            complement(2097..2897)
                     /locus_tag="PLD05_04230"
     CDS             complement(2097..2897)
                     /locus_tag="PLD05_04230"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOP76671.1"
                     /translation="MERMLLVAVDIGNSRIKCGIFYPEGWRRKSPEVNCVFSAGLYDP
                     AWRDFADPLFAAVAEVPRVTWWIASVNRPVTTDFLEILRAARPKDEVMLVSSSDLPLE
                     VAVPHPDRVGIDRLAAAVGANTLRDPNRPAIVVGVGTAVTVNAISKEGVFLGGAISCG
                     AAMSARALHEFTDLLPLIPTEWQTAPESIGKETPSALAAGIFWGLVGTVRELIERQTE
                     VLQGPPEVFLTGGGAKPLVGHLGDDVRFVENLTLIGIAAAARAVLDKG"
     assembly_gap    2997..3096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3109..4125)
                     /gene="obgE"
                     /locus_tag="PLD05_04235"
     CDS             complement(3109..4125)
                     /gene="obgE"
                     /locus_tag="PLD05_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOP76672.1"
                     /translation="MFVDEVEIFVQGGRGGDGCVSFRREKYVPKGGPDGGDGGDGGSV
                     ILVAEEGVDSLALLAHRKHWIAEDGERGGPADRHGRSGEALIIPVPPGTLVFDAEHNL
                     LLKDLQRPGEKVVVARGGKGGKGNAAFKSPTNRAPRECTPGTEGEKRRLRLELRLIAD
                     VGLVGKPNAGKSTLLSRLSRARPKIAAYPFTTRFPHLGRVVVDGERSFVLADIPGLIE
                     GAHRGAGLGHEFLRHIQRAGILLHLIEPEPYDGSDPISNYFSIRDEMEKFDPALVQRP
                     EIVVVTKADLPSVHSVAQKLEERINKPVLTVSAVTGENLHVLVETIWKTLQESRASRR
                     GSER"
     gene            complement(4241..4492)
                     /gene="rpmA"
                     /locus_tag="PLD05_04240"
     CDS             complement(4241..4492)
                     /gene="rpmA"
                     /locus_tag="PLD05_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOP76673.1"
                     /translation="MAHKKGQGSSRNGRDSNAKYRGLKRFGGQFVKAGSILVRQVGTR
                     FQPGLGVGVGRDYTLFALTDGYVKFDRGGRRVNVVPSLN"
     gene            complement(4561..5568)
                     /locus_tag="PLD05_04245"
     CDS             complement(4561..5568)
                     /locus_tag="PLD05_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76674.1"
                     /translation="MASVFRMTTGVALAGLLLTVCVSGGAQALEPAEAGDPSADPAAA
                     AQPEQPANEPCPEPPMPSPAETPKEDVLPVPEQILGVPRRLAPGVLIKIPPDIQVSET
                     HNRQDIVELVSIDPQLEWARNVDFRRNVYCLEFEFKLPRLITVDIPQPSGRLQQKPIW
                     YMVYRVTNRGGALRPVLEDDGTYRIESVDVPVLFIPTFYLEGKAASGEKRYPDRVIPL
                     AKPIIQAREDRAIQFFDSTETARTLQVGQSMWGVAMWEDVDPTINCFSIIVAGLSNAY
                     KWTDPPGAYKVGDPVGQGRVFQRKMLKINFWRPGDEFIVKEEYVRMGQPGAVDYEWIY
                     R"
     gene            complement(5767..6369)
                     /gene="def"
                     /locus_tag="PLD05_04250"
     CDS             complement(5767..6369)
                     /gene="def"
                     /locus_tag="PLD05_04250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOP76675.1"
                     /translation="MSESQKTLRLIQYPHPTLRYKAKPVRKVDSELFTFIREMFDLMY
                     EHNGIGLAANQVDLPYRLFVINLTADPSERGEELVFINPEIVRRSGSEEAEEGCLSLP
                     DVRAMVRRSKEVQIVAYTPSGDEINWRLSGLLARAFQHELDHLNGVLFVDHLSPSESL
                     SVRPILQDLEKEYAEALSKGQVRPLEDVMARLKELEVART"
     gene            6710..7729
                     /locus_tag="PLD05_04255"
     CDS             6710..7729
                     /locus_tag="PLD05_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOP76676.1"
                     /translation="MGRVFAQIRLIRDKAQPGEPGLTSTPVSWFVSFVLHALVLLLVA
                     LLWKENAQHGLSQEESREVGIALRTDNPEHRYLSESHRRGTENGQSGSSSQEKEGPSL
                     DDVILDSVPADPRKNLPAHHSSGLGVVNDPLSSAGGSTGVTGMLSGGGFGRSTGSAGS
                     ASLFGIQSPGNKFVYVFDRSGSMGGVGRTALDFAKAELISSLKTLNQTQQFQIVFYNE
                     RPLVFNPSGTPGRLAFATDENKRRAINFIQSVTASGGTQHDTAILLAIQLQPDVIFFL
                     TDADEPRLSDAKLEQIHRWANGIVIHTIEFGMGPRTSTDNFLSRLARQNGGQYVYIDI
                     TQAIR"
     gene            7840..>8374
                     /locus_tag="PLD05_04260"
     CDS             7840..>8374
                     /locus_tag="PLD05_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76677.1"
                     /translation="MITSLLMPLGNVRLAAVALQPTHASSSEKQSTQVTDFVEAVGAS
                     GVVQRLPGVIVDVTGKGVTLQGLDGSIRQIPCEKVIHIEANRSATYWESQQALKESRI
                     NEALSLLYQARSDEPRAWVRREMTAQIVRLLHANGRYGDAAREFLAAEFASDPSSPYW
                     SSIPLVWFPEVSLSSEIQ"
     assembly_gap    8375..8474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8475..8698
                     /locus_tag="PLD05_04265"
     CDS             <8475..8698
                     /locus_tag="PLD05_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76678.1"
                     /translation="AMLAYLKPPILWPENRRIAARCLWEVASLLAKDQTEFGGVARRL
                     QTERLYQELATSYPESPWSRQIPKSQGTN"
     gene            complement(8768..9340)
                     /locus_tag="PLD05_04270"
     CDS             complement(8768..9340)
                     /locus_tag="PLD05_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOP76679.1"
                     /translation="MLPAKDIKRGMIVSYQDAPCLIESVQVQTPSARGAATLYKFRAR
                     NLLSKQKVDFTLKGTEGLEEANFERRPVTFMYADADSVHFLDSVDYNEYVLPKEQLSD
                     ELNYLKEGLEGIFALVFEGACVGIQLPVAVELKIVQCDPYVRGASATGRTKPAVLETG
                     LVIQVPEYMSEGEVVKVDTRTGEFLSRVSS"
     gene            complement(9408..9905)
                     /locus_tag="PLD05_04275"
     CDS             complement(9408..9905)
                     /locus_tag="PLD05_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76680.1"
                     /translation="MSMFQDSNYHWRETCFVYFSRENRPTLQQVIRTLRTVGPQYQVM
                     NAQADQAGRFESITVIAPDAFSAMDICYLDGPEIVEEVEKQVKDLSASDLTAEERKQL
                     ARLKTCNARLDVFHFERLMDDDEDDESDEPGDFFDPSALIIVLEAFCKLTHGVAIDPQ
                     SGTFL"
     assembly_gap    10287..10386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10387..11554)
                     /locus_tag="PLD05_04280"
     CDS             complement(<10387..11554)
                     /locus_tag="PLD05_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOP76681.1"
                     /translation="MNEGNSTVLPDLADSSSQQVGNAQTSSEPSPEASAPSETGEKRT
                     TESPAPLEGPPSAPPVIVAITPEGLVVASQDPEALNQFENLVRRMVSQDVTQYGGPEL
                     VVYYLKNSKAESVASILDQILGGGTLTTSGSGGGSLIGEIARAAFGNVGGGLVGSLLG
                     IDTGTTSSTSSTSSTRTTSRIQITPDSRLNALIVQAYPQDLQLIEEILRVLDQPESPQ
                     DVAVQPKTIIIPVKNTQAEEIANILRSVYQDRLVTAASANRGPSPQEIIQLLRGGGRG
                     GPGGPGRNQPTEEVQRMSIGVDVRTNSLIISAPERLLNEVRQLVEQLDQKAATDSEET
                     TQVVTLQRASPESVRAALSALLGSGVQIRGSTSSSRVTSSSSTSSSGRSSSFSGR"
     assembly_gap    11640..11739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11740..14618)
                     /locus_tag="PLD05_04285"
     CDS             complement(<11740..14618)
                     /locus_tag="PLD05_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOP76682.1"
                     /translation="MKSRIGFRLTWGKTLLVLIVTLWQLSVCGVTAQAADYDVVGVLA
                     IAVEPDSAEQLGLTPNQLAQLQDLIADKEAEILASSSELRNLTPAERSARLADFRLAA
                     EEAGLRVLSAQQRAKLEQIRLKRYGLRVLTDPVVARRLQISPEQAATIQQTLAQRATN
                     GGVADNGGAGGDEIIQVLNESQRQAFRSMLLGELPASPAAPANGVVMNSEAQPVDLPD
                     DEEAPSVSADLDDDAPVGMATPVDAEPSEDIPPAPVANETATKSEESHNTASAAPSGE
                     SKDTDQPAATASQSAVEERTPESQTPSPPSDSQVPPGPAAGTTETANQPAIAPVEKSD
                     ESAAETPKEGEAKDHPETKPGENQPSTLAPAEPTKVEPPKVEDTQSGTSASPSPVVPP
                     PDAKGEGPAPQAVTVMPEPPKPAEPKPAVGGPSPGDVKGRFGERPGPLPPGLPGTIPG
                     MAPPPAPQPAAPRPEQVAGPGQTPSADQQIPQLPSADVKLRFNFRYQPWKEVLDWLAR
                     QAGLSLVMEAPPPGTFNYVDDHEFTPAQAIDLLNSVLLTKGYVLVRRERMLILFNVDD
                     GIPPNLVDTVSPEDLDNRGDYEMVSCLFQLRRMTPEEAEQEVKKLLGPQGSVVVLAKA
                     KQLFVTETAGRLKTIRRMLDAIENPSANDMVRTFRINPSTVDQVLSIIRQMFQIPADQ
                     TANADGTLRFAIDPSASRLLVSGSPEMLDRFSQLLQALDPSATDGGSALESTPQLEVY
                     PITSADPQMVLQVLQTLLAGLPDVRLAIDPKTGALVAWARPAQHATIRATLQQLQGEV
                     GQVEVIPLRVVDPQLAVLAISKLFGTGDSASGGAPTVDADLSTRMLLVKGTKGQIEQI
                     RQLLIKMGEPADTSGSANLSLMRTIPMDAATAQAVLDQMQNLWPQVRPNRLQVIRSVV
                     PADAGRPSPAANDVEQSRTGNEQTNGGIESQRIPLPSVMTSSSRLV"
     gene            complement(14916..16694)
                     /locus_tag="PLD05_04290"
     CDS             complement(14916..16694)
                     /locus_tag="PLD05_04290"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76683.1"
                     /translation="MTMTTGNVEGICIWDLPSQQAEARAFRQVWLRMARTVVKQALVT
                     AWLRIWLIILLGGGLWLGLFWVFAEGFQFLETAIPYSDLVEQIIRYVFGTFFMALMVM
                     LIVSAAILLYGGLFRSPDTLFLLTQPVRIERLFASRLFDTVILSSWAFIILSSPLLLG
                     FALVQEAHVLFYVLMVPYLIAFVYIPCCLGALFCLIVAQHVPRLKSFLVVTAIICLVV
                     FAVILLNWISWLPRNDALTVEWLQNMLNRLSVTQFRLLPSWWLTTGLMSAARGNIVDG
                     VMFLVVILANAMGLHALCVVVAGKYYRTVFFRLADSGRKKRNAHTGLVDHLLLHRVPR
                     VAPAIRQLIVKDIRLFRRDPVQWSQLLIFIGLLIFYFVNIRRMYYEQFYFAWMNLISF
                     LNVAVVALLLSTFTTRFIYPLISLEGPRFWILGLMPISRPAIVWSKVAFALCLCLIPC
                     CGLVFLSDLMLGVDALVHLAHQATTVMCCVGLTAIAVGLGARYPNFRAVSAARIASGF
                     GGTLNLVISTAFIIVVLGLGSLPAHVRIVAESPGLLPTVTARVEFNPLVVAWLNYGPL
                     GAVVFTIVIGGITLWVGLQHFRRLEF"
     gene            complement(16756..17646)
                     /locus_tag="PLD05_04295"
     CDS             complement(16756..17646)
                     /locus_tag="PLD05_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76684.1"
                     /translation="MIVFSDVTKRFGPKTAVEHLNLSIPRGELFAFLGPNGAGKTTTI
                     RMLVGLLRPNEGTVAICGFNTATQPREAARLIGYVPDQPYLYDKLSGREFLEFVGQLR
                     GLPLPVLKDRIDREIERFGMSEFVDDLTETYSHGMRQRLVFASAVLHDPSVLIVDEPM
                     VGLDPKSAKLVKDLLKSKAAEGASVFMSTHTLSIAEEIADRIGIIHKGRLIFAGSVEE
                     LRALRASDHASLEEIFLELTDEAATELSQSDPAWASKDPPSHSDPVEQPHSDWKNSEI
                     LAKTETKEVLGRAVEERSNT"
     gene            complement(17643..18581)
                     /locus_tag="PLD05_04300"
     CDS             complement(17643..18581)
                     /locus_tag="PLD05_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76685.1"
                     /translation="MYFSRAYLVSVGVFWLGAMGWLVMEKIVPTLLVGNPPRYSDIVD
                     SYRQQKAIGWTIQWRDREIGWALIRTEVLGDDLTEVKNVLQFRELPIHEIFPLIGQVL
                     AAELPVSRLRLRVETNLLFDPLKRLSSFQTRLKTPNSQEPLVVVRGSIDKGKLQLSIQ
                     SGVFVYDTELPFQSNTLLTDSFSPQATLPNLREGQKWTIEVYSPIQLPQQAVGARPLE
                     VVHAEVQGQEMILWQQELHKCWVVVYKSDPAESLNSRREPRGRMWVDSKGRVLKQEAR
                     VGGGKIVLVRLGEKETARLVRGLDAESWPTANKGIK"
     gene            18812..19546
                     /locus_tag="PLD05_04305"
     CDS             18812..19546
                     /locus_tag="PLD05_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76686.1"
                     /translation="MVPTLFLIALAGTAVDVGWKPTPDGRIEYIIQVTPEEVQNLNIG
                     DIIAASDVPPGLPPIAAYRIVVGRGPLPRELPDHPSTTEPRETIASDPRQDNDPSKVT
                     RVAQPAVGVFPADAEQLPAIPTEAGPTSGSSQPSDPAKARNASSEDAVPSNAAENAPD
                     RPQPENLSNDSSSQSHVLPVVAAILFALCLASVALAAWIATDYRRKYRELVKNVDSSL
                     LPPNDSQESPAPSEDTNTPKVQQSPS"
     gene            complement(19564..21672)
                     /locus_tag="PLD05_04310"
     CDS             complement(19564..21672)
                     /locus_tag="PLD05_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOP76687.1"
                     /translation="MQITRMRKYSYGLAKACTFGAISALLLTTIVDSFAYSQDFFGAL
                     NQNKFRVTGALTRVEPVGYILLVKAEIEPGWHIYSLTQKGGPVATKIDLAFPEGVAPA
                     GAFVVFPDPERKTEAAFPGLIVETHSGTVVWAVRLGIEHTLSPSKLAIEGSLKAQPCR
                     ADACLPPTTQKFTARWMDQGFVPQYVLDHLITKLKELAQETPPEGPDKTSAMSETLNA
                     GGNQTPNESGLTQLTAGKTQDDKELPWLPFTLARFRQLVGPEFQPERLQIQSSFSRRG
                     RWDLPLFIALGFLGGIILNVMPCVLPVIGLKLLAFLQQAGESRFKAFWLNVWYSVGLL
                     SVFALLAVLAITLQFGWGHLFRLPMFNVAMAAIVFAMALAFLGVWTMPIPGFVGSGKA
                     VELAQKEGAAGAFFKGVFTTILATPCTGPFMGSALAWSFTQPPLVVFTVFMSVGLGMA
                     FPYLVIGAFPELIRFLPKPGAWMETFERIMGFVLLGTVVFIFTFLDWPYVVPTVGLLF
                     GLWAVCWWIERAALRGRRIEKIRAWGEGLAFLVVVWIILFPGLPLGEKRITGLLGVME
                     RRFQERAFQVAWEAANQRNPRPALLDNGNPTTGGGYTVMVDCTADWCLTCKTLEATVL
                     NTERVKAVIRKNNVILLKADWTHAAPEVTQFLDWLGFRQVPVLAIFPAEKPNQPIVFT
                     GGYTQKDVIDALNRAGPSRN"
     gene            22201..22548
                     /locus_tag="PLD05_04315"
     CDS             22201..22548
                     /locus_tag="PLD05_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOP76688.1"
                     /translation="MKLILAIVQPPKLESIRGALERIEVSRLTLCDAQGYGRQRGHTA
                     SYRGHEYKAKLLRKVALEIVVNDDFLDRTINTIAHVARTGSEGAIGDGKIFVLPVEEA
                     IQIEDGTRGPGAV"
     gene            22567..22968
                     /locus_tag="PLD05_04320"
     CDS             22567..22968
                     /locus_tag="PLD05_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76689.1"
                     /translation="MTFRGIFLDRTSVSTTWKRKKFERSWDLACYRLVMPLFYQMIHG
                     RIPMETSDHSSEGSFERIDLTSAPDCGSDVRRGKGRPYLGILFRCCGIYLRIYLNRDG
                     TAFEGRCPRCLRVVRIGVSPDGSDDRFFSAF"
     gene            23140..24429
                     /locus_tag="PLD05_04325"
     CDS             23140..24429
                     /locus_tag="PLD05_04325"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOP76690.1"
                     /translation="MAKKNLVVAQSGGPTPVINNSLRGIVEAARDLDAIGTVYGAKHG
                     IEGVLKEELINLSSQPAEEIALLRYTPVAGSIGTCRYKLKPQQTEDFERVVEVFRAHN
                     VGYFLYIGGNDSMDTAQKIADLAHERGLDLVAVGVPKTIDNDVGDSEFQLVDHTPGYG
                     STAKYWTHMIQYANEENAGSCPADPVLVMQAMGRKIGFIPAAARLADPERQMPLLIYL
                     AECSCPLEKIADEVNDQLRRHGRCLVVISEGFDVGDVGEIKDAFGHTSYSSSKMTVCQ
                     ILVNYLNERGLPTKGAARGNVPGTDQRHSMAYASTVDLEEAYKVGQQAALLAASGQSG
                     YMATILRNPGPIYSARYDKVPLRVVANSERKFPKQWIAENGHDVTDDFIRYAKPLIGE
                     DMVNLPMVDGRQRMTRLQPIYAEKKLPPYVPQADRGK"
     gene            24512..25381
                     /locus_tag="PLD05_04330"
     CDS             24512..25381
                     /locus_tag="PLD05_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOP76691.1"
                     /translation="MISLPRQKDFLVGIDSDGCAFDTMELKHKECFIPNIIYYYNLQG
                     VSKYAREVAEFVNLYSKSRGINRFPALIETLERLRRRPEVKARGVKVVVPESLRKWMQ
                     SEKKLGNPALEQAVQATNDPELKQALEWSRAVNRSVEWMVHGVPPFPFVRESLEKLSK
                     VADIIVVSATPEEALKREWAEHGLDKYVRAILGQESGTKKDFLGLSSQYPSGHSLMVG
                     DAPGDQKAAEANGALFFPINPGEEEKSWQEFFEKGIDRFLNGSFSGDYQKELLAHFDQ
                     CLPIDPPWVEEAD"
     gene            25447..26904
                     /gene="gnd"
                     /locus_tag="PLD05_04335"
     CDS             25447..26904
                     /gene="gnd"
                     /locus_tag="PLD05_04335"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOP76692.1"
                     /translation="MNGQCDIAVIGLEVMGRNLALNIESRGYRVAVYNRTVEKMEKFI
                     EGPAKGKNIVGCRSLEELVAALAKPRKVMMMVKAGPAVDALIDQLIPLLEPGDILIDG
                     GNTHFADTERRTAYVESKGLLYIGTGVSGGEEGALKGPSLMPGGSEKAWPIVKPIFQA
                     IAAKVGPNKDIPCCDWVGPRGAGHYVKMVHNGIEYGDMQLIAEAYWLLKHGLGATNEE
                     LYDIFARWNEGELNSYLIEITRDIFSVVDKETGQYLVDLILDKAGAKGTGKWMSQLAL
                     DLGVPSTLVTEAVHARNLSALKEERVRASKILSGPPIQRYQGDRQEFIEQIRQALYAS
                     KIVSYAQGFVQLREAAKEHQWPLNFGSIAMLWRGGCIIRAVFLERIKEAFDENANLEN
                     LLLAPYFKQAVEKAQAAWRNVVCFALSNGIPIPAFAAALTYYDGYRSERLPANLLQAQ
                     RDYFGAHTYERVDKPGQGPFHTDWLSERRPPKNIG"
     gene            26973..27758
                     /locus_tag="PLD05_04340"
     CDS             26973..27758
                     /locus_tag="PLD05_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP76693.1"
                     /translation="MTTAEYLEDLFGLHGQVAVVIGGAGELGGALCEGILRAGAHVVI
                     ADLLEEACRRRLEQLREFSDKVSYCLVDVTKRESLENLLEFAKKIKGRVEILVNCAGI
                     NAGSSFLEASPEMWDKIMAVNLKGVFEACQVFIAHMLEAGGGSILNIGSVTSTLPLSR
                     VFAYAASKAAVVNLTKNIANEFGDKGIRVNAICPGFFPAEQNRRLLDEERVRNIINGT
                     PMHRFGEPHELIGAALLLLSPKAGSYITGAAFHVDGGFTAAWF"
     gene            complement(27775..28971)
                     /locus_tag="PLD05_04345"
     CDS             complement(27775..28971)
                     /locus_tag="PLD05_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP76694.1"
                     /translation="MASPEVKFWNSPAGFADPRSGHGSVGNGPNRPLASTVSTASDTE
                     DEFDIEDLQSARVRKRDLITTLQQLAILAEAGVNVATALQATIEEEKNSTLRSILKQV
                     KKDIESGQDFSAALSRYPKVFNNTVVALAKAGEKTGALGAMLARAAGYLQAEYETTAK
                     VRAAMVYPAIMLVMAVGVTVFLLTFVLPRFIPMFEARHVALPKITVMTLAASNFLRGH
                     WKTFLALAGGVFAILAIMRRSQSGREYLDRIKLSVPLLGPIYRRIAISRSCRTLSTVL
                     YAGVPILDGLNLAAAISENTIIRRAWLTVRDGIMEGRRVCDCMKTETIFPRPLVQMVA
                     AGEDAGNLDQVLGKVAEFYERELEVTIKTATSLIEPAMIIIMGIVVGTIGLSLLLPIF
                     SLSRPA"
     gene            complement(29062..>30408)
                     /locus_tag="PLD05_04350"
     CDS             complement(29062..>30408)
                     /locus_tag="PLD05_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOP76695.1"
                     /translation="PIVAPYRDIRRMIASLPNSSVFVIDDILDGTDVAEVTLVEETIE
                     DIGNLAEIAGQSPVIRLVNYILFNAVKEGASDIHIEPSERCVRVRYRVDGRLQKAIEL
                     PAHLGAPITSRIKIMAGMDISERRLPQDGRVNVLLENRKIDLRVSTFPGQHGEKTVIR
                     ILDTRHVSLNLDDLGFSEDVLGKLKAAIRAPNGIILVTGPTGSGKSTTLYACLNAISS
                     IEINICTVEDPIEYHLPLVNQFQVQDKIGLTFSKALRTLLRQDPDVIMVGEIRDEETA
                     RTAIQAALTGHLVLSTLHTNDAASAVTRLVNMGIESYLISAALNVVLAQRLVRRICPK
                     CKHPYDPPRPLRKALAGMGCTCEVFYRGVGCRNCRNTGYSGRIGIHEILVLNDELRDM
                     IAAGASAHQIRKAAEQQGMINIRTDGFRKVQEGLTTIDEVLTAVGDIQDLSRMSGQ"
BASE COUNT         6968 a         8528 c         7893 g         6619 t
ORIGIN      
        1 tcgagccacc ctcacccccg acccctctcc cgctgaccgg gagaggggag attctgggat
       61 aggctttaag atgattgtca cgacgcggca ggccaagagc gttcctcgcg gtaattctta
      121 gagtgtcgat tagttcgatc ccgcttcgta gataatccgc cgcaaatgtc tgacggcgca
      181 taccaccgcc gctggtacgt ctgatgactg ttctatcagc gggacagttc aaacaggtca
      241 ttccgggcag gacaggggct tgtgaccagc atggagttcc actccggtag gtccgatgga
      301 tagggataag gagcccttaa gaggcgagga ggagaccatg acgaggaaga aatacaccat
      361 ccgtgtcgta acgcggggtg ccgatggtga gatccgcatt cgcgactacg acagccccga
      421 agacctgctg aaacggcata cccaggttgg catcgacgac tgttgtaccg acctgagcgt
      481 ccgtggtctt cctgtcgtca agggccttat cggcccgatt ccggaagcgc ccgggattgt
      541 ccgctatgaa tccccggagg tgttcgagac gctgacgaaa gaatggtcca tgaccaaaat
      601 cacacgccgc cgtacgcgga cgcagcgacc agacctcaat gtgaaataat gctgggttgt
      661 tgggaacgtt cgggatggag cacccggcct cgtgccgggt gtcttcattt gaggatgtct
      721 ttgggagcaa acttcccggt ttcgtagaag taaatggctc gttccgagga gcaagcgctc
      781 aaccttgggg gatggactct ccctcccaca gctcacgata actttggcgt ctgcggatca
      841 gttgcggacg tcccccgtcg atcagcacct ccgcacaaag gggtttggcg ttataattcg
      901 aacccatgac aaatccgtag gccccggcac attcgataac gaggtaatcg cccaccgatg
      961 cttggggaag ccgccgcgtg accaccacac cgccttcctc ttgcgtaaag atgtcgcccg
     1021 actcgcacaa ggggccaccg acgattacgt cgtactctgg ccttgcgaaa ttccgatcac
     1081 gtgggacgac gctaatggga tggtaagccc cgtacatcac agggcgggca aggttgttaa
     1141 aaccggcatc aaccagataa aagagattat cggcctgttt cttcaccgcc ctaatctctg
     1201 tcactaagta gcccgcttcg gccacaagat accgccccgg ttcaatctcc aatcgcacag
     1261 gatgaccgaa cgccgattcc aacttcctcc gagttgtgtt ccaaagctca aaatagcgtt
     1321 ccagatcaat ccgctggtcc gaatcacgat agggtgtcgg aagcccgccg cctgcgctga
     1381 tggaccgcac acttggtccc acggcaaaag cgacttcttc catggcgcta cacaccttgg
     1441 ccaggtgctc aaagtcactt cccgatccga tgtgcatgtg aagccccgtg acgctcagtc
     1501 caagttcttt ggcctgtgcc agtgcctcgc caagttcttt aaaccatatc ccgtgtttgg
     1561 aaaactcacc accggtgttg gtctttcggc tatgaccgtg cccaaaaccg ggattgatcc
     1621 gcaaagtgat ctcgcgccct ggcgcccgct cacccaactg ctggatcatg tcgcgcgaac
     1681 cacagttcac gtgaactcct ttttccacaa ccagctcgag agaatcccga tcgaaaatat
     1741 cggcggtata gacaattggc gggggatcac ccacggctgc gtaaccggcg actaacgcac
     1801 gatgaatttc cccggcacta acagcatcaa ccagtgcccc caattgccgc agcaaggaca
     1861 ggatggcgag atttgaacag gccttttggg cgtagcggac aacatcaaac ttgcgaagtt
     1921 cgttaaaccg aagacgaatc gtttccgcgt catacacgta aagcggtgtt ccgaattgct
     1981 cggcaaggct aaccacatcg attccggcaa tgtcgcaacg tagtgttttt ctttcagcca
     2041 tatgtgttac cttttccatg atgtcgtatg ataacttcgc tgccacatgt gacttctcat
     2101 cctttgtcta acaccgctcg ggccgctgcc gcaataccaa taagtgtcaa gttctcaacg
     2161 aaacggacat cgtcacccag gtgtccgacc aagggtttgg ctccccctcc tgtcaaaaaa
     2221 acctcaggag gaccctgaag gacctcggtc tggcgttcga taagctcccg cactgtgccc
     2281 accaatcccc agaagatacc ggcggccaag gccgagggcg tctctttccc gatgctctca
     2341 ggcgctgttt gccattctgt tggaatgagt ggcaataaat cggtgaattc atgcaaggcc
     2401 ctggcagaca tcgcggcgcc gcaggaaatg gctccgccca gaaagacgcc ctccttcgaa
     2461 atagcattga ccgtaacggc ggtccctacc ccgaccacaa tggccggacg atttgggtct
     2521 cgcagtgtat tcgccccaac cgccgcggcc agccgatcaa tccctacccg atcaggatgg
     2581 ggaacggcaa cctccagggg caaatctgat gacgacacca acatgacttc gtccttgggc
     2641 ctagccgctc gaaggatttc cagaaaatcc gtcgttaccg ggcgattgac actggcaatc
     2701 caccatgtaa cgcgcggcac ttccgcaacg gcggcgaaca gaggatctgc gaaatcgcgc
     2761 cacgctggat cgtaaagccc agctgaaaag acacagttga cctcgggaga cttccttctc
     2821 cagccttcgg gatagaatat cccacacttg attcggctgt ttccaatgtc gactgcgacc
     2881 aacaacatcc gttccatgag gccgtaccat gcgatgaatc ctggcaaatc gtccgagata
     2941 atcctcccat tatggagatt cttcgcagtt cactgcaatg tccgtggata agttgcnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggt gcaagaagtt agcgttcgct
     3121 cccacgccgt gacgctcgag actcttgcag cgtcttccaa atcgtctcca ccagcacgtg
     3181 aaggttctcg cccgtcactg cggaaactgt gaggacaggt ttgttgatcc tctcctcaag
     3241 cttttgggct accgagtgga cactcggcaa gtcggccttg gtgacaacca caatttcggg
     3301 acgctgaacc agtgctggat cgaacttctc catctcgtct cgaatgctga aatagttcga
     3361 aatggggtcc gagccgtcgt aaggttccgg ctcaatgaga tgcagtagga tacctgcccg
     3421 ctggatgtgg cggaggaact cgtggcccaa acctgcgccg cgatgcgcac cttcaatcag
     3481 tcccggaata tcggccaaca cgaaactgcg ctcgccgtca accacaaccc gaccgagatg
     3541 gggaaacctc gtggtgaacg ggtaggctgc aatcttcgga cgtgcccgac tcaaccggct
     3601 caggagtgtg ctcttgccgg cattaggttt acccaccaat cccacatcag cgatcagccg
     3661 caattcgaga cgcaaccgtc gcttttcgcc ttcggtaccg ggtgtgcatt cccgcggtgc
     3721 ccgattggtt ggcgacttga acgccgcatt cccctttccc cccttcccgc cccttgccac
     3781 cacgaccttt tcgcctggtc gctggaggtc cttaaggagg aggttatgct cggcatcaaa
     3841 gacaagcgtt cccggcggga cgggaatgat aagggcctca cctgatcgcc catgccggtc
     3901 ggcaggccca ccacgctcgc cgtcctcagc gatccaatgt ttgcggtgtg ccaatagggc
     3961 cagactgtcg actccctcct ccgcaacgag gatgacactt cctccatctc ccccatctcc
     4021 gccatccggt ccccccttgg ggacgtactt ttcccgccgg aagctaacac atccgtctcc
     4081 gccacgcccc ccttgaacga aaatctcaac ttcatccaca aacattgtca gcgaacatgt
     4141 tgaaaaatgg cggccgacat cgtggagcta cggaaatacc gcgcacaaaa aaggagacca
     4201 actcggtctc ctaaagcagc cttccaacga aatcgaacaa tcaattcagc gaggggacga
     4261 catttacccg acggcctccc cgatcgaatt tcacatatcc atcagtgagg gcaaacagcg
     4321 tatagtcacg cccaacgcca acacctaaac caggctgaaa ccgggtccca acctgtcgca
     4381 cgaggatgct cccggctttg acaaactgcc ccccgaaacg cttcaagccc cgatacttgg
     4441 cgtttgagtc gcgtccgtta cggctcgaac cttgtccctt cttatgcgcc atggcttaca
     4501 cccattccat atcgaatcaa atggagaact ttcactttcc aaccgctata gaactcattt
     4561 ttaccggtaa atccattcgt agtcaaccgc cccgggttgt cccatgcgga cgtactcttc
     4621 cttaacgata aactcgtcgc ctggtcgcca aaaattgatc ttgagcattt tcctttggaa
     4681 gacgcggcct tgaccgacgg gatccccgac cttgtacgcc cctggcggat ccgtccattt
     4741 gtaggcattg cttaaccctg ccacaataat cgaaaagcaa ttgatcgtcg gatcgacatc
     4801 ttcccacata gccacacccc acatggactg cccaacctgc aacgtgcggg ctgtctcggt
     4861 ggaatcaaaa aattgaatag cccgatcctc ccgtgcttga atgatgggct ttgccaatgg
     4921 gatgacgcga tcgggatatc gcttctcgcc acttgccgcc ttgccttcga ggtaaaatgt
     4981 tgggatgaat aacaccggca catcaacact ttcgatccga taagtcccat cgtcttccag
     5041 gacgggacgc aaggccccac ctcggtttgt aacgcgatag accatatacc aaatgggctt
     5101 ctgctgcaaa cggccactgg gttggggaat atccacggta atgaggcggg gcagtttgaa
     5161 ttcaaactcc aggcaataca cattgcgtcg gaaatcgaca ttgcgggccc attcgagttg
     5221 cggatcaatg ctgacaagct ccacaatgtc ctggcggttg tgagtctccg agacctggat
     5281 atccggcggg attttgatca aaacgcccgg cgccaaacgg cggggaaccc ccaaaatctg
     5341 ttctggaacg gggagcacat cttcctttgg ggtttcggct gggctaggca tcggtggctc
     5401 aggacacggc tcattggcgg gttgctccgg ctgcgccgct gcggcgggat cggcagacgg
     5461 atccccggcc tccgcgggtt ctaacgcctg agcccctccc gagacgcaaa ccgtgagaag
     5521 caagccggcc agtgctacac ccgtggtcat tctgaaaacc gatgccatcg atgagtcctc
     5581 gcgttgctcc taacgaataa cacaacctcc ccaaccgacc tggctgacgg ggtgtgaagc
     5641 aaatcgccca tgccaaccaa ctatgcccct ctctctagtt taattagagc atatcctttc
     5701 gccaaattgt ctcaacgtgt cgtccacatt gctagccata cccctttaca acacgccgat
     5761 cgcgagctac gtacgggcaa cctcaagttc tttgagccga gccataacgt cttccagagg
     5821 acgtacctgg ccttttgata aggcttccgc gtactctttc tccaggtctt gcaaaatcgg
     5881 acgaacgctg agactttcgg atggcgaaag atgatcgacg aaaagcacgc cgttcagatg
     5941 gtcaagttcg tgctgaaagg cccgggctag cagccctgat agccgccaat tgatctcgtc
     6001 cccactcggc gtgtaggcga caatctgcac ctctttggac cgcctgacca ttgccctgac
     6061 gtcaggcaag ctcaaacagc cctcctcagc ctcttccgaa cctgaccgcc gaacaatctc
     6121 ggggttgatg aacactagct cttcccccct ttctgacggg tctgctgtca aattgattac
     6181 aaataaccga tagggaagat caacctgatt tgcggccagg ccgatcccgt tgtgctcata
     6241 catcaagtca aacatttctc tgataaaagt aaaaagctca gaatccacct tccggacagg
     6301 ctttgcctta tacctaaggg tgggatgtgg gtactggatt aatcgaaggg tcttctgtga
     6361 ttcgctcatt tcttgcgtca tctctgctta cgtaacgatt acgcgtctcc tgaggtcatt
     6421 ttgggccttg tcggcctatc cgcccccgac ggtgcggcca cctaacattc acggtgttgc
     6481 tactttcggc tgtatcggct ctctagagtt tccaaaatct tcactggcaa cgcgagcttt
     6541 gcctcgaact cggcttgcga acggttgata actattatat gccatccgtt taaaataagt
     6601 gcggatggcc gtgaattttt cacagcccgc tgtggcccat cgaaccggtg ctgcttgtcc
     6661 accgatcgcg ctcgggcccc ttttggaagt cggcggaaat gagctcgtca tgggtcgagt
     6721 atttgcgcaa ataagattga ttcgagacaa ggcacaacct ggcgagcctg gtctgacaag
     6781 tacaccagtc tcctggtttg tttcgtttgt attgcacgcc ttagtccttc tcctggtagc
     6841 cctcctttgg aaagagaatg cgcaacatgg gttgtcccaa gaagaatcac gagaggtggg
     6901 catcgcccta agaaccgata acccggagca ccgatacctt tcggaaagtc atcggcgggg
     6961 aaccgaaaac ggacagagcg gttcttcctc tcaggaaaag gaaggcccat cgctggacga
     7021 cgtgattctc gattcggttc cagccgaccc gcgaaagaat ctaccggcgc accactcttc
     7081 agggctcggc gttgtcaacg accctctctc ttccgccggc ggctcgacgg gggtgacggg
     7141 gatgttgtcc ggcggcggat ttggccgttc gacgggctct gcgggaagtg cctcactgtt
     7201 tggaatccag agcccaggaa ataagtttgt ttatgtgttc gaccggtcgg gaagcatggg
     7261 gggagtgggt cggaccgcgc ttgatttcgc caaggccgag ttgatcagca gtttgaagac
     7321 cctcaatcag acgcaacaat ttcagattgt cttctacaat gagaggccac tcgttttcaa
     7381 tccaagtggg acaccgggga gattggcgtt tgccaccgac gaaaacaagc gtcgggcaat
     7441 taacttcatc cagtctgtga cggcaagcgg cggcactcag cacgataccg ccatcctgtt
     7501 ggccattcaa ttacagccgg atgttatctt tttccttacg gacgcggatg agccgaggct
     7561 ttctgacgcg aagctggagc aaatccaccg ctgggcaaac ggaatcgtca ttcacacgat
     7621 tgagttcgga atgggcccca ggacaagcac agacaatttt ttgagccggc tagcccggca
     7681 aaacggtggt cagtatgttt acatcgacat tacccaagcg attcggtaat cgagaatcat
     7741 aaggtcatga gatttgcaac gggaaaacga cggcttcgcc cctgccctct cctactcggg
     7801 agcgatgcaa ccaaggggtt ttctttccag tgtctcatca tgattacatc gcttttgatg
     7861 ccgcttggga atgtgcggct ggccgcggtg gcgctccagc caacacatgc gtcttcttca
     7921 gaaaaacaat ccacgcaggt aaccgatttc gtggaggcgg tgggggcttc aggggtcgtt
     7981 cagcgtctgc caggtgttat tgtggacgtg accggaaagg gtgtcacctt acagggcctc
     8041 gatggttcga tccgccaaat accatgtgaa aaagtaatcc acatcgaggc aaatcgttcg
     8101 gcgacctatt gggaatccca acaagcgtta aaggaaagcc gtatcaatga ggcgttgtcc
     8161 ctgctctatc aagcccgttc ggacgaacca cgagcatggg ttcgccgtga gatgacggca
     8221 caaattgtta ggttgcttca tgcgaacggg cgatacggtg atgctgcgcg ggagtttttg
     8281 gcggcagaat ttgcgtccga tccaagttcg ccgtattggt catccattcc gctggtttgg
     8341 tttcccgaag tgagtttgtc atcagagatt caacnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnngggcaa tgctggctta tcttaaacca ccgatcctct ggccggaaaa
     8521 tcggcgtatc gctgcgcgat gcctttggga ggtagcgtcc cttttggcga aggatcaaac
     8581 ggagttcggg ggcgttgcac gccggttaca gactgagagg ctctaccaag aattggcaac
     8641 tagctatccc gagtcaccgt ggtcccgtca aatcccgaag tcccaaggaa ccaattgagc
     8701 gaccttttcc gcagggatag gttcagagcc ttctgacgtt tgtcactgct tgccgtgcct
     8761 gtttagttca cgatgacacc cgagagagga actcccccgt ccgtgtatcg actttgacga
     8821 cttcgccttc cgacatgtac tctggaacct gaatgaccaa cccggtttcc agaacagctg
     8881 gcttcgtgcg acctgtggcg gaggcacctc ggacgtaagg atcgcactgg acaatcttca
     8941 gctcaacagc caccggcagt tgaatcccaa cgcatgctcc ctcaaaaaca agggcaaaaa
     9001 tgccttccag tccctctttg agataattca gctcgtcgct gagttgctcc ttcggcaaga
     9061 cgtactcgtt gtagtccacc gagtcgagaa aatggaccga atctgcatcc gcgtacatga
     9121 aggttacggg gcggcgttca aagtttgcct cttccagccc ctctgtccct ttaagcgtaa
     9181 aatcgacctt ttgttttgac agcagattgc gggcacgaaa cttgtaaagc gtggcggcgc
     9241 ctcgcgcgct gggcgtctga acttggacgg actcaatcaa acagggtgcg tcctgatacg
     9301 aaacgatcat tccacgtttg atatcctttg ccggtagcat cgaaaaaatg tctccctttg
     9361 tgtcccaatc tccccgaacc tttgctttcc tctagtactt aaccaaatta caagaaggtg
     9421 cccgattgag gatcaatggc gactccgtgt gtcaatttgc aaaaggcctc aaggacgatg
     9481 atcagtgcac tgggatcgaa aaaatcacca ggctcgtcgc tttcgtcatc ctcatcgtcg
     9541 tccattaatc gttcgaaatg aaacacatca agcctcgcgt tgcaggtctt tagtcttgca
     9601 agttgtttcc gttcttccgc tgtcaagtca gacgccgaga ggtctttcac ctgtttttca
     9661 acttcttcca caatctccgg gccgtccaga tagcaaatgt ccatggctga aaaggcatcg
     9721 ggcgcgatca cagttatcga ctcaaaccgc cctgcctgat cggcctgcgc attcataacc
     9781 tgatattgag gccccacggt tctcaaggtc cttatgacct gttgtagggt cgggcgattt
     9841 tcgcgagaga agtaaacaaa acacgtttcc cgccagtgat aattactatc ctgaaacata
     9901 gacatggcgg tgttccatcc ctcggtttta tgtcggtccg ctttacggac tgtcaaaagt
     9961 ggtcctgttc ccccgtgcct aggcacagct gacagcgaaa acttatttta cgttctggcg
    10021 actaccctgc caaacccatc tgcgtcgaga atcgtcgaaa acgcattcgc gaaaaaagcc
    10081 cccactttca agcgagaaac gggagaaggt ggcgacgata accccatctc actgattcgc
    10141 gctcagctcg cgcaatgcac gtctctcagg acgcccattc gggcgggacc cgcgaagata
    10201 tgaagatgaa cctaacgccc gcccgaacgg ccggagaagc ctgacgatct tccgaactgc
    10261 gaggagcttc cgaaggggct tgagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnngtct gccagaaaat gacgagctcc gaccagaact gcttgttgaa ctggatgatg
    10441 tgacccgcga ggagctcgta ctaccgcgaa tttggacccc cgatcctaga agcgccgaca
    10501 aggcagctcg aacggattcg ggactggctc tttgcaaggt tacgacttgt gtcgtctctt
    10561 cggaatccgt cgctgccttt tggtcaagtt gctccacaag ttgacgaacc tcgtttagca
    10621 atcgctcggg tgcagaaatg attagggaat tggtccgaac atcaacgcca atagacatgc
    10681 gttgaacctc ttccgtgggt tgatttcgtc cggggccccc tgggccaccc cgcccaccac
    10741 cccggagaag ttgaatgatt tcctgcggac ttgggccacg attcgcgctg gcagccgtca
    10801 caagccgatc ttgatacacc gaacgcaaaa tattcgcaat ctcttccgcc tgggtattct
    10861 tgaccgggat gataatggtc ttcggttgaa ctgcgacatc ctgcgggctt tccggttgat
    10921 caaggacccg caaaatctcc tcaatcaact gcagatcctg cggataggcc tgcacgatga
    10981 gggcattcaa tctagagtcc ggcgtgattt ggatgcggct cgtcgttctc gtggaactcg
    11041 tgcttgaggt cgaggaagtt gtccctgtgt cgatgcccaa aagtgagcct accaatccgc
    11101 caccgacatt tccaaaagcc gcccgggcta tttcaccgat tagggaccct cctccggaac
    11161 cgctggttgt cagcgttccc ccgccaagaa tttgatcgag gatagatgcc acggactcgg
    11221 ccttcgagtt cttgaggtag taaaccacca actcaggacc accatattga gtcacatctt
    11281 ggctaaccat ccgccgaacg aggttttcga actgattaag cgcctccggg tcctgacttg
    11341 ccaccaccag tccttccggc gtgatggcca caatgacggg tggtgcactc ggcggacctt
    11401 cgaggggtgc gggagactct gtcgtacgct tttccccggt ttcgctgggt gcgctggctt
    11461 cggggctggg ctccgacgag gtttgtgcgt taccgacctg ttgcgagctg ctgtccgcca
    11521 agtccggcag tacagtcgaa tttccctcgt tcacgacttt tgactcctga gagacgaaaa
    11581 caacaggtgc ccacggtcgc ccggtaggcg agtcaccaga ggaagacatg acaccaaggn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccaggcggct gctggacgtc
    11761 atgacactag gcaagggaat tcgttgcgat tcaattcccc cgttcgtttg ctcgttgcca
    11821 gtccgtgatt gttccacgtc gtttgccgcc ggtgacggtc ggcctgcgtc agccggtacc
    11881 acagatctga tcacctgcaa ccggttgggt ctcacttgcg gccagaggtt ttgcatctgg
    11941 tcgagcacgg cttgcgcggt tgccgcgtcc atggggatgg tccgcatgag gctgagatta
    12001 gcactgccgg aggtgtcagc gggttccccc atcttgatca gaagctgacg aatctgttcg
    12061 atctgcccct ttgtcccctt gacaagtaac attcgtgtcg acaaatcagc gtccaccgtt
    12121 ggggcaccgc cggaagcgct gtcccctgtg ccaaacaact tgctaatggc aaggacagcc
    12181 aattgtggat cgacgacgcg gagcggaata acctccactt ggccaacttc accctgtaac
    12241 tgttgcaacg tagctcgaat cgtcgcatgc tgggctggcc ttgcccatgc caccagggcc
    12301 cccgtctttg ggtcaatcgc cagccgtacg tcgggcaagc ccgccaacaa cgtttgcaat
    12361 acctgcaaga ccatctgcgg gtcggccgat gtgatgggat acacttccaa ctgtggcgtg
    12421 ctctctagtg ctgacccgcc atcggttgcc gagggatcaa gtgcctggag cagttggctg
    12481 aagcgatcga gcatctcagg cgacccggat accaaaagcc tactagcgct aggatcaata
    12541 gcaaaacgca gcgtgccgtc agcgttagcc gtctggtccg ccggaatctg gaacatctgg
    12601 cggataatac tgagcacctg gtcaaccgtc gaaggattga tccggaaggt ccgaaccatg
    12661 tcattagctg agggattttc aatcgcgtcc agcattcttc gaatggtttt gagccgccct
    12721 gccgtttccg tgacgaatag ctgcttcgct ttcgctaaga cgacgacgct cccttgcggt
    12781 cccaagagct ttttcacctc ctgctctgct tcctcgggtg tcattcggcg gagctggaag
    12841 agacagctaa ccatttcgta atcccccctg ttatctaagt cttccgggga gaccgtatcg
    12901 accaaattcg ggggaatccc gtcgtccacg ttgaaaagaa tcagcattcg ttctcgccgc
    12961 acaaggacat agccctttgt caaaaggaca ctattgagaa gatcaatagc ctgcgcaggc
    13021 gtgaactcgt ggtcatcgac atagttgaat gtccccggcg gcggagcctc catgacaagc
    13081 gataagcctg cctggcgtgc aagccaatca agcacttctt tccacggttg gtagcgaaag
    13141 ttaaaacgca atttcacgtc cgctgacgga agttgcggaa tctgctggtc agcacttggc
    13201 gtttgaccag gtccggcgac ctgctccggt cgtggtgccg ccggttgggg agctgggggt
    13261 ggtgccatac cagggatagt gccgggcagt ccaggaggta gagggccagg tcgctccccg
    13321 aatcttccct tgacatcccc aggacttggc cctccgactg ctggctttgg ttcggcgggt
    13381 ttcgggggtt cgggcattac ggtcacggcc tgcggcgccg gtccctcacc cttcgcatcc
    13441 ggcggcggaa ccacgggcga tggagatgca ctcgtcccgg actgtgtgtc ctccactttt
    13501 ggtggctcca cctttgttgg ctccgcagga gctaaagtgc tcggctgatt ctcgcccggt
    13561 tttgtctcag gatgatcttt cgcttcaccc tccttcggcg tctctgcggc agactcatcg
    13621 ctcttctcaa cgggcgcaat agctggttgg tttgcggttt cggttgtccc cgcagcaggc
    13681 cccggaggta cctgcgagtc ggatggcgga ctaggtgtct gcgactcagg agtgcgttcc
    13741 tctactgccg attgggacgc ggtagccgcc ggctgatccg tatcctttga ttccccactt
    13801 ggtgccgcgc tcgcggtgtt gtggctctcc tcagactttg ttgcagtctc attggccacg
    13861 ggggccggcg gtatgtcctc cgacggttct gcatcaacag gcgttgccat ccccacgggc
    13921 gcgtcgtcat caagatcggc agaaacggac ggtgcctcct catcgtctgg caggtcaaca
    13981 ggttgagcct cggaattcat gaccacacca tttgctggag cggccggaga agccggcaac
    14041 tctcccaaga gcatcgaacg aaatgcctga cgttgtgatt cgttcaggac ttggataatc
    14101 tcatctccac ccgcgcctcc gttatcagct accccaccat tagttgcgcg ctgggcaagc
    14161 gtttgctgga tggtggccgc ctgttccgga gagatttgca aacgccgggc cacgaccgga
    14221 tcggtaagta cccgaaggcc ataccgcttt agtcttattt gttccagctt ggcccgctgc
    14281 tgggcgctca gcactctgag gcccgcttct tcagcagcca atcggaaatc ggcaagacgg
    14341 gcacttcgtt ccgccggtgt caaattcctc agctccgagg aagaggcgag aatctccgcc
    14401 tccttgtcgg caatcaggtc ctgaagttgg gctaattggt ttggcgtaag ccccagttgt
    14461 tctgccgaat ctggttcgac agcaatcgcc aggaccccca ccacgtcgta atccgccgct
    14521 tgcgcggtta ctccgcagac ggacagttgc cagagcgtca caatgagaac tagcaatgtt
    14581 ttaccccacg tcaaacgaaa accgattcgt gatttcatcg gcgtatatcc tcaaagacaa
    14641 aaacccaacc cttgcagttg cccgaaaaaa ccatttcggg tcagagcaac atcctcaaaa
    14701 cgcctcggtt tttctttttc aagcaacact tgatgattgc cagcggaagg aagctaaacc
    14761 caaccctgat cgtaagcaga aagcctctac ttcgatcgta gcatcgaaac ccccataatt
    14821 gctaaccatt accaagtgag aaagtttcgt tttgtgtgcg tctctagcgc atcacctata
    14881 tagggcattt cgtgctgcac ggatcgtggt agcggtcaaa actcgaggcg acggaagtgc
    14941 tgcaaaccaa cccagagcgt aattccccca ataacgatcg taaaaaccac tgccccaagc
    15001 ggcccataat taagccacgc cacaacgagg ggattgaact cgacccgggc cgtcacggtc
    15061 ggcagtagcc cgggactctc tgccacaatt ctaacgtggg ccggtaatga tcctaggccc
    15121 aagaccacaa taatgaatgc cgtactaatg actaaattaa gggttccacc aaatccggag
    15181 gcaatccggg cggccgacac ggcacgaaaa ttgggatacc ttgcccctag cccgacggca
    15241 atggcggtaa gccccacaca acacattact gttgtggctt ggtgagccaa gtgaaccaaa
    15301 gcgtccaccc ccaacattaa atcgctgagg aacaccaacc cgcagcaggg gataaggcac
    15361 aaacaaagcg caaaggcgac tttgctccaa acaatcgccg gccgtgagat cggcataagt
    15421 cccaaaatcc aaaaccgggg accttcaagg ctaatcagcg ggtaaataaa tcttgtggta
    15481 aaggttgaca gcagcagggc gacaacggcc acattcaaga agctgattaa gttcatccac
    15541 gcgaaataaa actgctcata gtacatccgc ctgatgttga caaaataaaa gatcaaaagc
    15601 ccgatgaaga tcaacagctg cgaccactgt accgggtccc gccggaacaa ccggatgtcc
    15661 ttcacaatca attggcgaat ggcgggggcc acccggggaa cgcggtggag gagtaaatga
    15721 tccaccaagc ccgtgtgggc attccttttc ttccgtcctg agtccgccaa ccggaaaaag
    15781 acagtccggt agtactttcc cgcaaccaca acgcataacg cgtgaagtcc catcgcattt
    15841 gccagaatga ccaccaagaa catcacacca tcaacgatat tgccccgggc ggcagacatg
    15901 agccctgtgg tcaaccacca acttggcaac agacggaact gcgtaacact tagccgatta
    15961 agcatattct gtagccactc cacggtcagg gcgtcattcc tcggaagcca actgatccaa
    16021 ttgagcaaaa tcacggcgaa aacgaccaag cagatgattg ctgtgacaac tagaaacgac
    16081 ttcagccgtg gtacgtgctg ggcaacaatg agacagaaca gggcacctag acagcacggg
    16141 atatagacga acgctatcaa atacgggacc ataaggacat aaaacaggac atgggcctct
    16201 tgcaccaggg caaaccccaa tagcagggga cttgatagga tgatgaaggc ccagctactc
    16261 aggatgaccg tatcgaagag ccgtgaggca aaaaggcgct caatccgcac gggttgagtc
    16321 agaagaaaaa gggtgtccgg agatcgaaaa agtccaccgt aaagaaggat ggctgccgac
    16381 acgatcagca tcaccatcaa ggccataaaa aacgtcccga agacgtagcg gataatctgc
    16441 tcgacgagat cgctgtaagg aatggccgtc tcaagaaatt ggaacccctc cgcgaagacc
    16501 caaaacagtc cgagccacaa tcctccgccc aacaggataa tcaaccagat tcggagccac
    16561 gcggtgacca acgcctgctt gacaacggta cgcgccatcc gtagccacac ttggcggaac
    16621 gctcgcgcct ccgcttgctg gctcggcaag tcccaaatac aaatgccttc cacgttaccc
    16681 gtcgtcatag tcatcgattg atttccaaac ggaccccggc agagtctttg ccagtttcct
    16741 cacttgttgg ggatttcacg tgtttgagcg ttcctcaacg gcgcgcccaa gcacttcctt
    16801 tgtttcggtc ttcgcaagga tttcgctgtt tttccaatct gaatgtggtt gctccaccgg
    16861 gtcggaatga ctgggtgggt ccttggacgc ccacgctggg tcactttgtg acaattctgt
    16921 cgcggcttcg tctgtaagtt ccaaaaagat ctcttctaac gaggcgtggt cacttgctcg
    16981 cagagcccgt aattcctcta ctgagccggc aaaaatgagt cttcccttgt gaatgatccc
    17041 gatccggtca gcaatttctt ccgcaatact caaggtatgg gtggacataa acaccgatgc
    17101 gccttccgcg gccttgctct ttaaaaggtc cttcaccagc ttggcactct tcggatcgag
    17161 gccaaccatc ggttcatcaa cgatgaggac gctcggatca tgcaagacgg ccgaagcaaa
    17221 gacaagccgc tgccgcatcc cgtgggagta cgtctcggtc agatcatcca caaactcgct
    17281 cataccaaaa cgctcaatct cccggtcaat acgatccttg agtaccggca ggggaagtcc
    17341 ccgaagctga cccacgaact caagaaactc gcgtccggat aacttgtcgt aaagatacgg
    17401 ttggtcagga acgtagccaa tcagccgggc ggcctcacga ggctgcgttg ccgtattaaa
    17461 accacaaatc gccacggtgc cttcattggg acgaagcaaa ccgacgagca tacgaatggt
    17521 cgtcgtctta cctgccccat taggtcccaa aaacgcaaac agttcccccc gcggaatgct
    17581 aaggttcaaa tgttccactg ccgtttttgg tccaaaccgt tttgtaacgt cagaaaagac
    17641 gatcatttga ttcccttatt tgcggtcggc cacgactctg catccagccc acgaaccaat
    17701 cttgccgttt ctttttctcc caaacgaaca agcacaatct tccccccgcc taccctagcc
    17761 tcttgtttca atacccgccc ttttgagtcc acccacatgc gaccccgcgg ctcacggcgg
    17821 gagtttagcg actctgcagg gtcgctctta tacacaacga cccagcattt gtgcagttct
    17881 tgctgccaga gaatcatctc ctgaccctgt acctctgcgt ggacaacttc caagggtctc
    17941 gcccccacgg cttgttgggg aagctggatc gggctgtaca cctctatggt ccacttctga
    18001 ccctctctca aattaggcaa agttgcttga ggcgaaaacg aatccgttag cagcgtgttc
    18061 gattggaaag gcagttccgt atcatacaca aatacgcccg attgaatgga aagctgcaat
    18121 tttcctttgt caatgctgcc ccgcacgaca acaaggggct cctgggaatt aggcgttttg
    18181 agacgcgttt ggaatgatga aagccttttc aagggatcga acaagaggtt agtttccacg
    18241 cgcaagcgca accggctcac cggcaattca gcggccagga cctggccaat caaaggaaat
    18301 atctcgtgga tcggtaactc gcggaactgc aagacgtttt ttacctccgt gaggtcatcc
    18361 ccgagaacct ccgtccgtat cagcgcccag ccgatctccc tatccctcca ttggatggtc
    18421 catccgatcg ccttttgttg gcggtaacta tcgacaatgt cagagtaacg gggagggttt
    18481 cccactaaca gggttggcac aattttctcc atgaccagcc aacccatggc tcccaaccag
    18541 aagaccccaa cacttactaa atacgcacgg ctgaaataca tatgaaaagg caccttctcc
    18601 catccgaaac ccatgacccc acttgatcat tttaccaaac ttgtaagatg aggaaacaat
    18661 tacccgaagg tcgtgcggtt gcatcaaacg tgacaatgaa tcacaatcca aagtggttgt
    18721 cgttgcccgg gcgagatttg ccccgttgtt acagacgcct gagatgccaa agggcaggcc
    18781 tggcttttag gataaaccga tcactgcacg tatggttccc acgctattcc tcatcgcttt
    18841 ggcagggacc gcggttgacg tcgggtggaa gccgacgccc gacgggcgga ttgaatacat
    18901 tattcaagtg acccccgaag aagtgcaaaa tctgaatata ggagacatca tcgccgccag
    18961 cgatgtgccc cctggattgc ctccgatcgc cgcctatcgg atcgttgtcg gccgaggtcc
    19021 tttgccgcgg gaattgcccg atcacccctc cacaacagaa cctcgtgaaa ctatcgccag
    19081 tgatccgcga caagacaatg atccaagcaa agtcacccgt gtggcgcaac cggccgtagg
    19141 tgtcttccca gccgacgcgg aacagctccc agccattccc accgaggctg gtccgaccag
    19201 cggcagctca caaccgagtg acccggccaa agcacgcaat gcgtcatcag aagacgcggt
    19261 accctcaaat gccgccgaga atgcacctga tcggccacaa cccgagaatc taagcaacga
    19321 ctcttcatcc cagtcgcacg tcctcccggt agtggccgcg atcctcttcg ccctctgttt
    19381 ggcttctgtt gcccttgcgg catggatcgc taccgactat cgcaggaagt atcgagaact
    19441 tgtcaagaac gtcgattcaa gtctcctgcc cccaaacgat tcccaggaat cccctgcccc
    19501 atccgaggac accaacacgc ccaaagtgca gcaatcgccc tcatgaaatc aacacacggt
    19561 gagttagttc cgtgatggac cagccctatt gagtgcgtca atcacgtcct tctgggtgta
    19621 gccgccggta aagacgatag gttggttggg cttttctgcg ggaaatatgg ccaagacagg
    19681 aacttgtcgg aagcccagcc aatccagaaa ttgggtcacc tcgggggcgg catgggtcca
    19741 atcggctttc aaaaggataa cattattttt gcgaatcacg gcctttactc tttcagtgtt
    19801 aaggaccgtg gcctccaagg ttttacaggt aagacaccaa tcggctgtgc aatccaccat
    19861 cactgtgtaa ccgccgccgg tggtcggatt tccattgtca agcaaagctg gccgaggatt
    19921 cctctggttc gcagcttccc aagccacctg gaaggcacgt tcctgaaacc gccgctccat
    19981 cacaccaagc aggcccgtga ttcgtttttc ccctagtggg agacctggaa acaagatgat
    20041 ccaaacgacc accaagaacg cgaggccctc gccccatgcg cgaatctttt ctatgcggcg
    20101 acctctaagc gccgcccgct caatccacca acacacggcc caaagcccaa aaagcagtcc
    20161 tacggttgga acaacatacg gccaatccag aaacgtaaag atgaagacca cggtccccaa
    20221 tagcacgaag cccatgattc gctcaaacgt ctccatccaa gcccccggtt ttgggagaaa
    20281 acggatcagc tctggaaacg ccccaatcac cagataggga aacgccatcc ctaatcccac
    20341 actcatgaag accgtgaaga cgacaagggg cggttgcgtg aatgaccagg ctaaagccga
    20401 gcccataaaa ggaccggtgc aaggcgtggc caagatggtt gtaaaaaccc ccttgaaaaa
    20461 ggcgccagct gccccctctt tttgggcaag ttcaacggcc ttgccggatc ccacaaaccc
    20521 cgggattggc atcgtccata ccccgagaaa tgcgagcgcc atggcgaaga cgatcgctgc
    20581 catggccacg ttgaacatgg ggagtcgaaa gaggtgtccc cagccaaact ggagcgtaat
    20641 ggccaacacg gcaagcaggg cgaagaccga cagtaggccc acactatacc aaacgttgag
    20701 ccaaaacgcc ttaaagcgac tttcccctgc ctgttggaga aacgccagga gttttagtcc
    20761 aatgaccggt aacacgcacg gcatcacgtt caaaataatc ccccccagga aacctagggc
    20821 gataaacagc ggcaggtccc accgtccccg ccgagaaaac gagctttgga tctgtagccg
    20881 ctctggctga aactcaggtc ccacgagttg tcggaaccgg gccaacgtga atggcagcca
    20941 cggtagttcc ttgtcatctt gcgtctttcc cgccgtcaat tgcgtaagac cagattcgtt
    21001 tggagtctga ttcccaccag cgttcaaggt ttccgacatg gcggatgtct tatcgggccc
    21061 ctcggggggc gtttcttgcg ccaattcctt cagcttggtg atcaggtgat ccaggacata
    21121 ctgcggcaca aagccttggt ccatccaccg cgctgtgaac ttttgtgtcg tcgggggcaa
    21181 acaggcatct gcccgacatg gttgtgcttt aaggcttccc tcgatagcga gtttcgatgg
    21241 ggaaagagta tgctcgatac ccagtcttac ggcccacaca acggtcccag aatgggtctc
    21301 tacaattagc cccggaaaag ctgcctctgt cttgcgttcc ggatcaggaa agacgacaaa
    21361 cgcacctgct ggagctaccc cctcgggaaa agccaaatca attttcgtcg caacaggccc
    21421 gcccttttgc gttagtgagt agatatgcca acccggttcg atttcagcct tcaccaggag
    21481 gatgtaacct acgggctcga cgcgcgtgag tgcaccggtc actcgaaact tgttttggtt
    21541 aagtgcacca aaaaagtctt gactgtaagc aaacgaatca acaattgtgg tcagcaacag
    21601 cgccgagatt gccccaaacg tgcaggcttt tgccagaccg tacgagtact ttctcatcct
    21661 tgttatctgc atgaaacaac cgtctcctgc gactccgcaa ggaaaatttt cacgatggat
    21721 aaagccaaga aggcttacta tgccggccat tcgcaccaaa ccgatgcgta caaggcattt
    21781 tctcatctta tgtgtaagga tggtcgaccg gccaggggcg ttacaatccc gctttcatct
    21841 tcccatcttg aaaatctcga cggttctcat aggcggtctt catgagcaat gcccactcgg
    21901 catttgagag gtaaagcccg gacctactat gttgcccaag ctgtgtatat ttcctggtcg
    21961 ttaacagcgg cgagtttcgc aaggaccacc cagctttgcc ccctggcagt ttcactttcg
    22021 cacaaagacg atcattctcg gtcagaatga atttgaatca acaaaactgc ctctctggcc
    22081 attgtgtgcc gaaaacaaac acagcgcttg acgcagtagc aggcaagaat atgattggac
    22141 ggacatcaag ccgatattcc acatgattgt ttgtttcggg aagtttcgaa aagagacaca
    22201 atgaagctaa ttttggctat tgtccaacct ccgaaactgg aatcgatccg aggggccctc
    22261 gagcggatcg aggtctcacg tctcacgctc tgcgatgccc aggggtatgg gcgacagcgc
    22321 gggcacacgg cgtcgtatcg gggacatgaa tacaaggcaa aactcctgcg aaaggtggcc
    22381 cttgagattg tggtcaacga tgactttctc gaccgcacaa tcaatacgat tgcccacgtg
    22441 gcgagaaccg gttcggaagg cgccattggt gacggaaaga tctttgtgct tcccgttgaa
    22501 gaggcgattc aaatcgaaga tggcacacgg ggcccggggg ctgtttgacg ccgaagaagt
    22561 atcattttga catttcgcgg aatctttctc gacagaacct cggtttccac gacatggaaa
    22621 cgcaaaaaat tcgaacgaag ttgggacctc gcttgctatc gcttggtaat gccgcttttt
    22681 tatcagatga tccacggtcg tattcccatg gaaacttcag accattcatc cgaaggctca
    22741 tttgagcgca ttgacctgac cagtgcgccc gactgtggca gcgatgttcg gcggggaaag
    22801 ggtcgcccct atcttggcat tcttttccgc tgttgcggaa tttacctccg catttacttg
    22861 aatcgtgatg gcacagcgtt tgagggccgt tgcccccgat gcctgcgagt ggtccgaatc
    22921 ggcgtttcac ccgatgggag cgatgatcgg ttcttttctg cgttctgaag cggacaagtc
    22981 tctcttccgt caggtcctcc gcccaaaacg ggctagccgc cggttgtgag ctcctcgttt
    23041 tgccgataaa atcgcagttt cgagtgttga ttgggcaagc gtgcaagcga ccagagcgac
    23101 aaaattggca tatggagcgt cgtagagcga ggtaggtcta tggcaaaaaa gaatctcgtc
    23161 gtggcccaaa gtggtggccc aacgcccgtc attaacaaca gccttcgcgg gattgtggaa
    23221 gcagcccggg atcttgacgc catcggtacc gtttacggag ccaagcacgg gatcgaaggg
    23281 gttctcaagg aagagctgat caatctctct tctcagccgg cagaagaaat cgcattgctt
    23341 cgctatactc ccgtggcagg atcgatcgga acctgtcgct acaaattgaa gccacagcaa
    23401 acggaagatt tcgagcgcgt ggtcgaggtg tttcgcgccc ataatgttgg atacttcctc
    23461 tacattggcg gcaacgactc gatggacacc gctcaaaaga ttgctgacct ggcgcatgag
    23521 cggggacttg acctcgtggc agtgggtgtt ccgaagacca tcgataacga cgtcggagac
    23581 agcgagttcc agttggtcga tcacactcca ggctacggct caacggcgaa atattggacg
    23641 cacatgatcc agtacgcgaa tgaagaaaat gctggttcct gcccggctga tccggtcctg
    23701 gtcatgcaag cgatgggtcg aaaaatcggc tttattccgg ccgccgcccg cctagcagac
    23761 cccgagcgac agatgccgtt gttgatttac cttgccgagt gctcgtgccc cctcgaaaag
    23821 attgcggatg aagtcaacga tcaattgcgg cggcacggcc gctgtctggt tgtcatcagc
    23881 gaaggttttg atgtcggcga tgtcggcgaa attaaagatg ccttcgggca tacctcatat
    23941 agttccagca agatgacggt ctgccaaatc ctggtcaatt atttgaatga gcgtggtctg
    24001 ccaaccaagg gtgcagcccg cggtaatgtc ccgggaacgg atcaacggca ttcgatggcg
    24061 tatgcctcca ccgttgactt ggaggaagcg tacaaagttg gccagcaggc ggcattactg
    24121 gcggcttcgg gtcaatccgg ctatatggcg accattctgc ggaacccagg cccaatctat
    24181 tccgcacgct acgataaggt tccgcttcgt gtggtggcca atagcgaacg aaagttcccc
    24241 aaacagtgga tcgccgagaa tggccacgat gtgacggatg actttatccg ttatgcgaaa
    24301 cccttaatcg gcgaggatat ggtgaattta ccgatggttg acggtcgcca gcggatgacg
    24361 cgactccagc ccatttacgc ggaaaagaaa cttccgccct atgtgccgca ggcagaccgc
    24421 gggaagtgaa taggatgtgt ggagcggatt tccgccggtg tgatgacaat cacgtgtttg
    24481 ccaacagttg tcgttgatcg tggagtgccc catgatttca ttgccccgac agaaagattt
    24541 cctggtaggt attgactccg atggttgcgc tttcgatacg atggagctca agcacaagga
    24601 gtgtttcatc ccaaacatca tttattacta taacctccaa ggcgtgagca aatacgccag
    24661 agaggttgcc gagtttgtca atctctattc caagagtcgc ggcattaatc gtttcccggc
    24721 gctcattgag acccttgagc gtctccggcg gcgtccggaa gtaaaagcac gcggagtcaa
    24781 agttgtcgtc ccggaaagcc ttagaaaatg gatgcaaagc gaaaagaagc ttggcaatcc
    24841 tgccctggag caggctgttc aagcgacaaa cgatcccgag ctcaagcagg cactggagtg
    24901 gtctcgggcg gtcaatcgtt cggtagaatg gatggttcat ggagtgccgc cgttcccatt
    24961 tgtccgcgag agccttgaaa aattatccaa ggttgccgat atcatcgtgg tttcagcaac
    25021 tcccgaggaa gcgcttaaaa gggaatgggc tgaacatgga ttggataaat atgttcgggc
    25081 aattctgggt caggaaagcg gcacgaagaa ggatttcctg ggccttagct ctcagtatcc
    25141 gagtggccac agtttgatgg ttggggacgc cccgggcgac caaaaggcag cggaagccaa
    25201 cggtgccctt ttcttcccga tcaaccccgg cgaagaggaa aagagctggc aagaattctt
    25261 cgaaaaaggt atcgaccggt tcctcaacgg aagtttttcc ggcgattatc aaaaggagct
    25321 tctcgcacat ttcgatcaat gtctgcccat cgatccgcct tgggtcgagg aagccgactg
    25381 attcgactta gggtccagtt tcaataccta tcgatttagg ccactcttgt gatgtgaggt
    25441 gcgaacatga acggtcaatg tgatattgcg gtgatcggcc tggaggttat ggggcgtaac
    25501 ttggcgctca acatcgaaag ccgcggctat cgagttgcgg tctataaccg cactgtggag
    25561 aaaatggaga aattcatcga ggggcctgcc aagggaaaga acattgttgg ttgtcgctcc
    25621 ctcgaagagc ttgtcgcagc ccttgccaag ccgcggaagg tcatgatgat ggtcaaggcg
    25681 ggaccggctg tcgatgcctt gattgaccag ttgatcccac ttctggagcc gggtgatatc
    25741 ttaatcgacg gtgggaatac ccatttcgcc gacaccgaac ggcggacggc gtacgtggaa
    25801 tcgaagggct tgctctatat cggaacgggt gtttccggcg gcgaggaagg cgctctcaag
    25861 ggaccaagtt tgatgccggg cggcagtgaa aaggcctggc ccatcgtcaa gcctatcttt
    25921 caggccatcg cggccaaggt tggcccgaat aaggatatcc cctgctgtga ttgggtgggg
    25981 ccccgtggag ccggccacta cgtcaaaatg gtccacaacg gtatcgaata cggggatatg
    26041 caactgattg ccgaggcgta ctggctcctc aaacacgggc tgggggctac aaacgaagaa
    26101 ttgtacgata ttttcgctcg ctggaacgag ggggaactga atagctatct gatcgaaatc
    26161 acacgcgaca tcttcagtgt ggtggataaa gaaacgggtc agtacctcgt ggacttgatc
    26221 ttggacaagg ctggtgccaa gggaacaggc aagtggatga gccagcttgc tctcgacctc
    26281 ggggttccaa gtacgcttgt caccgaggca gtccatgcac gaaacctctc ggccttaaaa
    26341 gaggaacgag tgcgtgcctc taagattttg agtggtccac cgatccagcg ctatcaaggc
    26401 gaccgacaag agtttatcga acaaattcgt caggcccttt atgcctccaa gattgtcagc
    26461 tatgcccaag gatttgtgca gttacgagaa gcagccaaag agcaccaatg gccgctcaat
    26521 tttggcagca tcgccatgtt gtggcggggc ggctgtatca ttcgggccgt gttcttggaa
    26581 cgaattaaag aggcatttga tgaaaatgcc aatctcgaaa acctgctcct ggccccgtac
    26641 ttcaagcagg ctgtcgaaaa ggcccaagct gcgtggcgca acgtcgtgtg ctttgccctc
    26701 agtaacggta ttcctatccc cgcctttgct gcggcgttga cgtattatga tggttatcgt
    26761 tcggaacgct tgcctgccaa tctgttacag gcgcagcgag actacttcgg agcgcataca
    26821 tacgagcgag tggataagcc ggggcaagga ccgttccaca ccgattggct gtcagagcga
    26881 cggccaccga agaatatcgg ctaattttgc ccaggttgcc aaatcgtggc ataaaacgtg
    26941 atgactggcg tgctaatggc gaggacataa ccatgacaac tgcagaatat ctggaggatt
    27001 tgttcgggct tcacggccaa gttgccgtgg tgattggggg tgccggagaa ttgggcggtg
    27061 ccttgtgcga ggggatcctg cgagcgggag cccatgtggt cattgccgac cttcttgaag
    27121 aagcgtgccg ccgccgtctg gagcaactcc gcgaattcag cgacaaggtc agttactgtc
    27181 ttgtcgatgt gacaaagcgt gagtcgctgg agaacctact cgagtttgcc aagaagatca
    27241 agggacgcgt cgagattctg gtcaattgtg ccggaatcaa cgcgggaagc tcgtttctcg
    27301 aggctagtcc cgaaatgtgg gataaaatca tggcggtcaa tctcaaaggg gtctttgaag
    27361 cgtgccaggt tttcattgcc cacatgttgg aagcaggtgg cgggtcgatc ctcaatatcg
    27421 gctctgtcac gtcaacattg ccgctttccc gtgtctttgc ttatgccgcc tccaaagcgg
    27481 cggtggtcaa cctcaccaag aatatcgcta atgagttcgg agataagggt attagggtca
    27541 atgcaatttg cccgggcttc ttccctgcag aacagaatcg ccgacttctc gacgaggaac
    27601 gggtccgcaa tatcattaac ggcacgccca tgcatcgctt tggagagccg catgagctga
    27661 tcggggccgc cctgttgctt ctgtcaccaa aagccggaag ttacattacc ggtgccgcgt
    27721 tccacgtcga cggtggcttt actgctgcgt ggttctaatt cagcggaggt gattttacgc
    27781 gggacggctc aagctgaaaa tcggcagcag caacgatagg ccgattgtac ccacaacaat
    27841 ccccatgatg atgatcatgg cgggttcaat gaggctggtg gctgttttga tggtgacttc
    27901 gagttcgcgc tcgtagaatt cggcgacctt gccaagaacc tgatctaggt tgccggcatc
    27961 ctcgccagca gcaaccatct ggacgagggg gcgaggaaag atcgtctctg tcttcatgca
    28021 gtcgcacaca cgacgtccct ccatgattcc gtcgcgaacg gtgagccacg cccgtcgaat
    28081 gatagtgttc tccgatattg ccgccgccaa attaagacca tcgagaattg ggacgcccgc
    28141 gtacaggact gtgctcagtg tgcgacagct ccgagagatc gcaatgcgac gataaatcgg
    28201 tcccaaaagg gggacggaca atttgattcg atcaaggtat tccctcccgc tttggctgcg
    28261 tcgcatgatt gccaggatcg caaacacacc acctgccaaa gccaaaaacg ttttccagtg
    28321 cccccgcaaa aagttcgatg cggcaagcgt cataacggta attttcggga gcgcaacgtg
    28381 tcgcgcctcg aacatcggta tgaatcgggg aaggacaaac gtaagtagga aaaccgtaac
    28441 acccactgcc atcaccaaca tgatggcggg atacaccatc gcggcgcgaa ccttcgccgt
    28501 ggtctcgtat tcggcctgta gatagccagc ggcacgtgca agcattgctc ctagcgcccc
    28561 tgtcttttcg cctgccttgg ccagggcaac aacagtgttg ttgaaaacct tgggatagcg
    28621 tgaaagagcc gcggaaaagt cctggccact ctcgatatcc ttcttaacct gtttaaggat
    28681 acttctcagc gtgctgttct tttcttcttc gatagtagcc tgaagagctg tcgcaacatt
    28741 gacgccggct tccgcaagaa tggcaagctg ctggagtgtc gtaatcagat cccgtttacg
    28801 aacccgggcc gactgaagat cctcgatatc gaattcatcc tcggtgtcgc tggccgtcga
    28861 aactgtcgag gctaacggcc gatttggacc attccctacg ctcccgtgtc cggaacgcgg
    28921 gtcggcaaaa cccgccggcg aattccagaa cttcacttct gggcttgcca tagtccgtgt
    28981 attcccccta cgagcacttc tgattggtgg ccattaccga cttcaaggtt gcgtcgataa
    29041 gggcttttta aggcagtctt gttactgccc ggacatgcga gacaaatctt ggatatcccc
    29101 cacggccgtg aggacctcat caattgtggt gaggccctct tggacctttc ggaaaccgtc
    29161 cgtgcggata ttgatcatcc cctgttgttc ggcggctttg cggatctgat gtgcactggc
    29221 cccggctgcg atcatatccc gaagttcatc attaaggacc aagatttcat ggatcccaat
    29281 ccggccgcta tatccggtgt tccgacagtt gcggcatccc acaccgcgat aaaacacctc
    29341 gcacgtacac cccattcccg caagggcctt acgaagaggc cgcggaggat cgtatgggtg
    29401 cttgcatttg gggcaaatcc tgcggacgag ccgttgtgca agaacgacgt tgagcgccgc
    29461 agagatcaaa taggactcga tccccatatt cacaagtcgt gtcaccgccg atgccgcatc
    29521 gttcgtatga agggtgctca acacgaggtg acctgtgagg gcagcttgga tcgctgtacg
    29581 ggctgtctcc tcgtcgcgaa tttcgcccac catgatcaca tccggatctt gccgcaacag
    29641 cgtgcgaagc gccttggaga acgtgaggcc aattttgtct tggacctgga attgattgac
    29701 cagtggcaga tggtattcaa tcgggtcctc gaccgtacag atattgattt ctatggatga
    29761 aatggcatta agacaggcat aaagggtcgt agattttccg ctaccggtgg ggcccgtgac
    29821 taagatgatc ccgttgggag cccggatggc tgccttcagc ttgccgagaa catcttccga
    29881 aaaccccaga tcgtcaagat ttagcgaaac atggcgcgtg tctaagatcc gaatgaccgt
    29941 cttttcgccg tgctgcccag gaaatgtgct cacacgtagg tcgatcttgc gattttccaa
    30001 aaggacattc acgcggccat cttggggaag tcgccgttcg ctaatgtcca tgccagccat
    30061 gattttgatt cggctggtga tcggcgcacc gagatgggca ggcaactcga tggccttttg
    30121 caaacgacca tccacgcgat acctcacacg aacacaacgc tcgctcggtt cgatgtgaat
    30181 atcgctggcc ccctccttaa ccgcgttgaa caaaatatag ttcacaaggc gaatcaccgg
    30241 agactggccc gcgatttcgg cgaggtttcc gatatcctca atcgtctcct cgaccaacgt
    30301 gacctctgcc acgtcggttc catcgaggat atcgtcgatc acgaagacac tcgagttggg
    30361 taacgacgca atcatccgcc gtatatcccg gtagggggca acaatcgg
//
LOCUS       DAOSUT010000030        30318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1024_length_30318_cov_9.434887, whole genome shotgun sequence.
ACCESSION   DAOSUT010000030 DAOSUT010000000
VERSION     DAOSUT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 30318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30318
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1024_length_30318_cov_9.434887"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6236)
                     /locus_tag="PLD05_04355"
     CDS             complement(<1..6236)
                     /locus_tag="PLD05_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOP76696.1"
                     /translation="MKLWDTLRDFSSKYRPSAKGSRKKSEKKPRTHRPRDLRVERMED
                     RVLLSINPATLDRVWQDTLQRAIEQAADLDRYSDAQLQSTTDWVVGIGDDINPAQVAT
                     LLGAQYLPTPTTIKGAYVFRFSSSVDWQTAIQRLQTSELVNYFYPLVPQQRSLCLIPN
                     DPLFVQQWHLLNTGQSGGVPGADLNVVTVWDAYQGDGVVIGIVDDGLDTGHVDLLPNY
                     RSDLSYDYYAGDADPQHELPNDTGHGTAVAGTAAAAGNNATGVAGVAFDAQIAAIRLI
                     SGATTDLQEAQALSHRQDVISIYNNSWGPAGPGDIGFSVFAGPGPLTLAAIQQGVTTG
                     RNGLGNIYVFAAGNDGYLLDNVNYDGFANLRYVIAVGALNENGQQAFYSEPGACLFVV
                     APGGEMADPNDVGSGIITTDWTGPNGSSTGDYTGVQGTSFAAPAVSGVIALMLQANPN
                     LTYRDVQYILAQTARKVDPSDPDWTTNAAGYHINHKYGFGAIDAAAAVQAARNWKPVL
                     PEIALDSGIFQVNQVIPDNSQVGISSTISMASDINRVEWVEVTVDITHLSPGDLEIVL
                     VSPSGTQSVLATPHIHNPFDTGVQWTFTTNRNWGETSGGDWTLIVRDRVGGVVGRLNS
                     WRLTVYGQQVPGTPPDLVAVIPNEGSLIEEGAELLVAPRELILRFNEGQQLDPRTLGA
                     IQFVRAGGDDSFAEGNEVVVPIGYIGIGDKPNEVIVRFAENLPDDKYRLVIKGTGANP
                     LMNLDSPPLPFRYDPDLKTGRDLVMEFDINVGPQVLAVVPQPVYRGDFEITAPAGNQI
                     TDGQTFRVGDGTYQVTFEFDTGNGVRAGNVPIVYASGDSASLIAQKIGTAILNQGLNL
                     TVLVNGAVVTLRGSQVSVDRQNSPLRVKSLYQERNVIEVYFNNDKLDRNAAQDPRFYQ
                     LIFTRETLTPSDDLVVTPASVTYDAAKNLVRLVFAQDLAHYGTGAARLRIGSAYQPIQ
                     TWNMSEGADAASSFYAATVISGFGTGNLGQSVIISGSIVTPIAYNLEWPGAIDEPGHR
                     DLPNYPPPSIQGEDHFGGESSPDGSTQIPSVAYYFPTGSFNLITEAQKQRTREIFEIY
                     GQYLGIQFYEAETGGLAIITGDLALMGLQSAPGGVAGVAGGGMALMDYAENWGASEFG
                     GGWFQVAMHEIGHLLGYGHSYDLEPFVIMGRSEDAQSAVGSFEPVFPGVADIIHGRHM
                     FRPDNMDIDMYRFDLNERGLVDIEVLAERLNNSSLLDATITLYREVQTLQKYVVIENG
                     VPVEKEFTQTTYEVVARNDDFYSEDSAISVYLPAGRYYIGVSASGNTQYDPTLDNSGI
                     GGVTQGEYKLRVTYKPGGVDPNNPDTFREPNLANPSRLIDSTGVYFDGDLDGIPGGDY
                     NFWFTVQPESQTIFVDKLSGSSAPTGSIDNPFSTIAAALAVAQPGTIVRIVGNNFAND
                     DPSNPATLQDNIPYEIGRRLVDNAPLSDGVRMDVPKGVTVMIDAGAMIKLFRSNIMVG
                     SIAEGIDRSGGALQVLGTPFHSVYFTSYYDESLGRDPEPTNSTTPLSGDWGGIVFKND
                     LDYQFIRAYDPASGLPPRQVLEDQGIFLNYLSHADIRYGGGEVIVNGVRSVYNPVHMV
                     EARPTVVFSRITKSADAAMSADPNSFADTHYQSWDPNQPYTVEYDRVGPMIRGNYVVD
                     NTINGLYVRIKTPAGGATKKLTTFARWDDWDIVHYVPENLIIAGTPGGPLMRPSSAPD
                     VTLVDDFHLRAPAASPQLDGQYFSIFDGRTRVVFEFDAGDGVVTGRVPVSYTSTSTAQ
                     QIAASLAAAINAARLARGLEVAATSSGDTVTLEQRGAQLKVEGLGNREARIDARLMVD
                     PGVVVKLQGSRIETEIGAQIIAEGRAGFEETVPGYKVVFTSVLDTRYGAGGTFNTTQN
                     TSGQQPAPGNWAGIAFAPLSEGSIDQAIIAYAGGQSAIEGGFAGFNPIEIRQAHVRIA
                     NTRFANNGANSAGDRNGRGFISPATIYVRGAQPVIVNNLFINNQGHVLSIDVNALNAW
                     EVPDWGRSTGFIDAFSQYDGNFGPLVRENRMTGNGINGMQVRAGTLNGQGVWDDTDIV
                     HVVYG"
     gene            6939..7283
                     /locus_tag="PLD05_04360"
     CDS             6939..7283
                     /locus_tag="PLD05_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76697.1"
                     /translation="MKTQVLMWVGATVFLLACGAFLWAAAEPGLIGPRVTQPTTQTPG
                     WIVTAAVGDNRQQIVIADPAQQSLAVYHVDLTTGRIALKSVRHVRYDLLMTEFNTDSP
                     SPRELEALLKSP"
     gene            7345..8514
                     /locus_tag="PLD05_04365"
     CDS             7345..8514
                     /locus_tag="PLD05_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOP76698.1"
                     /translation="MAQKFYTTREAAELLGIAPADLNSMRERNEIRGFRDGMDWKFRA
                     EDIEALRLKLRADATRGAPHQTPSSDEEILLLDDDLKPGDLGTSGSGTVVGGLSGLDA
                     GKSDVKVAGSDVNLAGSGVGISGKPEESSSSVSPFDELAPLETPAETGKSPVGTGSDS
                     AVNLAGEGLEDDDVVIGGSGSGSDITLGSDSGISLMDTNDSGISLEGGLELVPSEESL
                     ELDEDDMLTLAAGADTEAPTQLRTDEEFMLTPFEEAESEETESGSQVIALDTEPATGS
                     SPGMAELLGAEAAAPLGVAPTIPGAVPGAAAPGIGAFPGPVPGVAPVMEPSMVVPVGE
                     APWGTLWNTMLIVCTVLLALCGMMCFDLVRNMWSWNGPYSLNSTIMDAILSVLPK"
     gene            8893..11136
                     /locus_tag="PLD05_04370"
     CDS             8893..11136
                     /locus_tag="PLD05_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76699.1"
                     /translation="MSRVRLRRPDHPLPLQFFLALIEFLASLRLAVTLLTLLILVLAV
                     ATFIETSYGAAAARFAVYRAWWFTVLGGLLAINILFAAIVRFPWKRYQTGFVITHAGI
                     LVLLAGAYWTALEGIDAQLSIYEGMIGHIAYQDTVHFEIDLTKKSRSPNQDNPSAGST
                     ENAADSRPSERRALRVAVPFHCGPFNWCDYSSGLRHLLWSPPGRLPVLPWQLAWRDKG
                     VIFTEGDLSLEVLDYYADSRRVRGGYLAGYIKANTATSAQGTWFRPWEPIELDVQSRR
                     TEAGITTAIGSRVTLSNGIRVVLTAARSQAELDGFLLGTPVEPLDHEGQVVLVHNQIR
                     YVLPVRQLRAATGDTPLEGSGLRITHFEKDVPLQAVQLQLKNDEGVEDEVILFADLPE
                     FNRQGQKLAVAGAYWVNFPSLLSDVGQAVRLHPRATTGATRARVDLCQGPGGKLYYRI
                     WQSPQVLKTGEINRDDRESILLVGNDLDVRLVVNTFVPWDPEHPEAETVVPLPFRKGA
                     RMTQPRVKVRLSAGGKTHETWLAVLPRGADRPIDAEEIATVASDTEQMRVTVKYDAVD
                     VGFRLKLREFQRKLDPGTNQPSYYASLVDLYVPERIAKEHGFENPRWKDQTIIDPSDG
                     EPLQLVHPRILITLNHPANIVDPITGRSYRLYQESFSGPFRPGDPIYEQVVGKEKGPP
                     ELYLSTLSVNYDPGRGLKYAGSLLIIGGIVLMYLMRVYIFRPRVRPPFRNEQLEQSVA
                     AGPVSVT"
     gene            11341..14370
                     /gene="ccsA"
                     /locus_tag="PLD05_04375"
     CDS             11341..14370
                     /gene="ccsA"
                     /locus_tag="PLD05_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOP76700.1"
                     /translation="MRRLLFAVHQYFRIVVLAWLCPVVIGMSATAGADDRPIDWSSWK
                     RLPLFEGGRIMPLDTFARRIVEKLTGKPQPILGPPMTPDGQLDRQHPLFPDGLLRKWP
                     AVELLFSWLAESEKWEDVPFLQASHQDLRERLLKVPLVDASGKRLSYVSPRDVENSME
                     FEAYLRDLSQRRREVESRGQRFRMSGLDLHAARLWDAYATFRLLTYQPRLDSRPWFPP
                     NVKVSHPIPRRLESLIDSWTEIEPQLQQLADLGVFSSGQSQTAGPGEPGEAPSGDQSG
                     SQEHLVARARAEFAPLLRAAESEEVTVAELDEALTRLQPLLREIALCVESLQNRAFSV
                     EAPDATKQLRLDDLRSNIRLLAEKSRRLADEVEVCVPMLYDTGVGPHVLPAMIPACLN
                     SATNGDEPVSPWLSLGAILYGSKNVLRGYDIDRVNQVRSFFQMAAAAYADRRSHDRTR
                     RVEENLDQFSAALAKLGRSIEAERQRLPVDESHRELLALTAYPPAGFTDWEVFYNEFD
                     PFRWSWIINLLAFGILLLGVGRLRQPSLWIGVLVLTVAQVVTIYGFGLRVAISGWAPV
                     TNMFESVVFTSLVIGLLGVWFVIRPLVGKGIREGWEWVSLPHEPLTGKREARTWQDKG
                     TFVNVLRGAGVLARLALGGLLFYYLAVVPYDVGKSRAVFDLVPDITRSLGSPTFSSIV
                     MNLLGWFVGMCVLLSVVWLVPRFAVGMILSLLWTIPVDLKTAELGPRLNEVVLRRPFA
                     VAAAGLAFLAAVVAYYTPVWDKGIEPLQPVLRDRLWLFAHVLTITAGYGAGLLAWGLG
                     NISLLYFLFGRYQAVPRGEIIVHGEAHRSVDGGGRDDGLTISPPLSCFTLATYMYKAI
                     QFAVILLTAGTILGGVWADRAWGRFWGWDPKEVWALISILVYMALLHGRYIGWVNTFG
                     LAAGSVFGMISVVWAWYGTNYLMPAGLHAYAGEGGGGGLYVVGAFTANLLFVGLAWMR
                     YRAEMAKGQGAVDARRGEETEGHPDESGIASTSVSGQ"
     gene            complement(14455..16038)
                     /locus_tag="PLD05_04380"
     CDS             complement(14455..16038)
                     /locus_tag="PLD05_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76701.1"
                     /translation="MRDSVAKVMLIGILLLIWTVPLALILGFTLWWRPGMGIRTECDR
                     IAHAIGFSCAIERYQTPRPGVKRYSHLTLRNTLTGSPVCVIENALSTRKSNKPHIDAS
                     GVSLSEEAWLRLWEICEEWLLGKRDFAAETFTLTASEIQVVPKLDEARPNPQPLVLCS
                     SLQTEFSSPSRQRAIKCRIVCSAEDQRQPPLPDLRPSDGPLQSTLSMVWQSTCKDGQF
                     YLTKQVRVEEPGIPLVVLKPLWNGLHEIPSSVRWAGTIEATVSHSCESLSCWSATISG
                     KLLWSREEATQSSPSLFDCLDAEGSVLLNRVVFINGTLKSADITVHAGPGQIKRAFLT
                     FLHDSFHTSVQAYEGNQSSMMQRGDPSWPLGSLHPGKPLPFEVPSDLALMQANQQGAH
                     PFRETDQLAGILQGDDVPFQHLVLRFDCDETGVRLMPADGGKLSWVLLESGAGWGVTV
                     SEETLGKRVTWGQILNNLTPPQQEPWPDHRWQQAFVQFLLRCVSVDSVLGGPKNGTDA
                     SASVGVLPTLAPSHTDRGM"
     gene            complement(16171..17319)
                     /gene="aroC"
                     /locus_tag="PLD05_04385"
     CDS             complement(16171..17319)
                     /gene="aroC"
                     /locus_tag="PLD05_04385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOP76702.1"
                     /translation="MLRYWTAGESHGKGILALVDGFPAGVELNLEIINRELLRRQGGF
                     GRGGRQRIEQDQAEILSGWWRGVTIGSPIAIWVANRDYKIEKMDDLTRPRPGHGDLAG
                     AVKYFSSIRGILERASARETVGRVAAGALARQLLSACQIDLLGYVVEVGPIKFPAKEG
                     HLDMLRQLRDSSELYTLHPEGEEEAKDYIRKCQKEGDTVGGVVEVRVENVPFGLGTHA
                     QWDRKLDGRLAQAVMAIQAIKGVEIGLGFEAARRPGSQVHDPIHFDPSQVNSRTLGFV
                     RATNHAGGLEAGMSNAQPIVLRAAMKPISTLARPLESVNLETKQPETAAYERSDVCAI
                     SAASVIVENVVAFEIAAALVDKFGGDHIEEIRQRIKLFEQMNRERLGL"
     gene            17784..20126
                     /locus_tag="PLD05_04390"
     CDS             17784..20126
                     /locus_tag="PLD05_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOP76703.1"
                     /translation="MRASQAAMLANVVRDVFYWACFSIFMTSVISQVGVSLASEPGIG
                     QIETLPGTEPLDWSGDIASRMIDAIDAFLLRQTEHTAKLRQQAWASVFSPDQWPSDWL
                     QAKRERLAQILGVKDPRVSPVQMTILSPPGQSRPLAEGAGYDVYAVSWDVFTDLKAEG
                     ILLYPKHDQPKACVVAIPDADVSPEALIGLELGVPPASQYARRLAESGCVVVIPSLIS
                     REISKRGNVSLTHREFIYRPAYELGRHIIGYELAKILAAIDYFDSAFPTLGGNFGVIG
                     WGEGGMLALYAGALDARLGVVGVSGYFGPREHLWQAPVDRNVFGLLKDFSDAEISLLI
                     YPRSCVIEAARGPEVTIPPKLGGAPGELKTPSLEEVKNEFDRANRILQQAGKSFHLSL
                     VPSENGSGPYGTEPFLRAFLASFGTPITLSAEADPPRRIRDDPWRATRTERAIAELDR
                     HIQRLLVESPEIRKRFWAKLDTSSLERFSQSVEWYRKYFYDEIIGRFDIPLADAKPRT
                     RKVYDTLSWEGFEVVLSVFDEVFAYGILLIPKGIKPGERRPVVVCQHGLEGRPQETIE
                     GDHRAYHNFAARLADEGFVVFAPQNPYIFGDRFRTLQRKANPLGKTLFSIIVPQHQQI
                     VNWLSTLEFVDPGRIAFYGLSYGGKTAMRVPPLVPQYCAVICSADFNEWVWKNASTKS
                     RYSYVWTGEYEIFEFALGETFNYAEMATLIAPRPFMVERGHFDGVAPDETVAYEYAKV
                     RFLYNALLKIGDRTEIEWFAGPHEIHGVGTFQFLHKNLRF"
     gene            20825..21862
                     /locus_tag="PLD05_04395"
     CDS             20825..21862
                     /locus_tag="PLD05_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOP76704.1"
                     /translation="MDRTHRLLVSQVAVPYLRRHLRRLSRALRGVPSAKDPEAVHQTR
                     VACRRLRAALRVFRNDIGKRTTRRWRKVFRKLGRVFGAARDLDVEIASMLGQMGQVRE
                     ENILHGLAFWLAHLEEQRHRKQPELIRAIRKFRRKAAILEMGQWIKRQSREMVAAGSS
                     QGGLPVSITKQHRKLLSERLADLLRTAQSLEDPQDIEGHHAMRIAAKKLRYSMEALAP
                     CLGVIGEVTIEALRSLQSHLGEIHDYDVWVERIRQVLDMISGGKFDGPRWLNPDRFMP
                     GLVFLQNKYQELREVRFRQLQNLWHDEKIQGLWQRLQASGFQAISEDNAKYADGARNR
                     EDHPDVKTQHP"
     gene            21958..23532
                     /locus_tag="PLD05_04400"
     CDS             21958..23532
                     /locus_tag="PLD05_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOP76705.1"
                     /translation="MSDLVDSHMTTNEGEDTVHPVAVIDIGTNAIRMVIAQVFPSGEF
                     EVLERLQRPIRLGKDTFSRGRIGAQSMRSVVAILRDYQAVMNSYGVQQVRAVATSSLR
                     DAANADNVLDRIYLACRLNVEIIGIAEEGRLTVAAVREVVEDLPEVNQGKTLIVDVGG
                     GSTILTMLQEGEIVNSIGLRFGSVRILEMLSLQSEPPERVAEVLQQHIRTQIANVERS
                     LTLQDCDTFVAVGGDVRFAARVVGHPTRSEKLFVVEKDQFRSLLRQCSRHTPNQLAHK
                     YNLPFIDAETLLPALMTYWELLCRTSAERVIVSQVSMRDGLLLELAREATGRKDNALQ
                     EGIVRAAEAVGQRYRVDLDHARLVAGIAIKLFDFLVADHGLGPRQRLLLQVAALLHEI
                     GGFISASAHHKHSYYIISNTEIFGLNRLETQIVAHVARYHRRSPPKPSHLDYMALSRE
                     QRVIVNKLAAILRIADALARGNVRQAEDVEFHHEADELIITLHNIENLVFQKRAIAVK
                     SDMFEDVYGMKVRLQE"
     gene            23789..25936
                     /gene="ppk1"
                     /locus_tag="PLD05_04405"
     CDS             23789..25936
                     /gene="ppk1"
                     /locus_tag="PLD05_04405"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOP76706.1"
                     /translation="MKKHTLSSNPELFINRELSWLEFNERVLQEGLSDSVPLLDRLKF
                     LAIVGTNLDEFFMIRVASLKQLKKAGKTRRDVAGMTPDEQLAAIGARVHKMVADHGRG
                     IREVFGKLNEKGIQVLAPEEWSDNDRRQLRAYFDRELTPTLTPLAMSDLVPAPALAGL
                     QIHLGIVLLPTQHKKDEVKIAVVPVPQKTPRFVALPSEGKQRFALVEDVIAAFVGELF
                     RGYRIAHVSPFRITRDADVSIQDDDASDLLNTVEEAVLARRRRAAVRLEIRPPVDQRL
                     YDFLVSSLQLEENDVYLVDGFMGTSFLYEIVNLPGFDHLREPEWPPQPVRDLQGWDDL
                     WDAITDHDVMLFHPYETFSPVIEFVKQAATDPSVLAIKQTLYRTSGDSPIVEALEHAG
                     RNGKEVTVLVEVKARFDEERNVQWARRLEDAGCHVIYGVAGLKTHAKAALVVRREHGR
                     IRRYVHLSTGNYNDKTARIYSDIGLMTCHPEITADISSFFNLLTGYSEAVGWQRLTVE
                     PGQLKERFISLIEREAEVATADHPGLIMAKVNSLQDKDICVALYRASQAGVKIKLNIR
                     GICCLRPGIKGVSENIEVRSIVDRFLEHARIFYFANGGNEEVYLSSADWMERNLERRL
                     ELVFPVVDPRLQRRLIEILHTYFADNVKAWELLPDGTYRRVSGHGPRVRAQEVLYRQI
                     VEVSRASPRELRYRPLKSDRAAEEQRQSDTKSR"
     gene            complement(25946..27346)
                     /locus_tag="PLD05_04410"
     CDS             complement(25946..27346)
                     /locus_tag="PLD05_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOP76707.1"
                     /translation="MGKKNGKKHHFVAVDVGATKILAVVCDPCGVIRSRDRRETPRSG
                     KPEDVIASIESAIDTVVRKSDLYFSEISAIGVAIPGVVNPKRGEILRTPNSCLSGVSL
                     EPHLTAKFGKRVVLGNDCNFGTLGEAWLGAGREASSVFGIFVGTGIGSGFVRKRKLWR
                     GAREVAGEIGHIVMGMGGPVCGCGNRGCFEALAGRNAIEREIREAVARGESTILTDLL
                     QGDLSVIKSRVLRMALDQKDPLVTRVISRASEVIGYACLTVRHLLDPEWIVLGGGVVE
                     ACGDYMWETIKRIIDADQLPGAQQRGGICLSALGDDAVVLGGVVAAARAVGCNVFKLV
                     RNVEYPQVKMTSRDTLKVDKKTFTNDVVILSDGRVKKWPPEKKGDRPNSGSSTADLAE
                     TLCRGGTELVILGTGPDGDTWDVTPFFQYFEKRRISVLALPTAQAVGSYQRSKSRKTA
                     FFRLGGATPSTSENRT"
     gene            27615..29234
                     /locus_tag="PLD05_04415"
     CDS             27615..29234
                     /locus_tag="PLD05_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76708.1"
                     /translation="MWCSRANKRVQSVIGQGVLVSFLVTVTVVVSATRAFAQGKADNL
                     WQLCRDKAAIHRFSTFFTAQNVRDYLATDEGRQSAIAWCRQTGVTRVFIESFRDGYLV
                     PKDLLAQASHVFRAAGFDVSGCVTTTNVGKPSTGWQIISCYTDRATQDKLAEIFKYTA
                     SLFDEIMIDDFLFCDCQCAECDAARKSRTVNIGERSFPVDSDTWSAYRCELMVQVSRQ
                     LILEASRSVNPNVRIIIKYPQWYDQFHERGYDVVRQTADFDLIWVGTETRDYDNPRWG
                     RKVQYEAYFIMRWLGGIGGEKCGGGWFDPFGTTEHTYLEQARQTVLAGAKESMLFCYG
                     ALQRDTGPRNVEVLRQHIPDLLRTAEQVRRRSIIGIAAYKPPHSSPGKEAYVFDFVGM
                     LGLPLVPCHEFPREAKAAFFSVHALTDPRFEVELSRLVERGVPILLTDGLARQLKNQE
                     LLKKSNVHVLQVQGDPHRLLKFSEDEVNQLRQAMLGPWSITVRGPNKLGVYIFDDGSY
                     VLENFNDQAIRVEFNGVQHDIPARDWVQVWK"
     gene            complement(29250..30254)
                     /locus_tag="PLD05_04420"
     CDS             complement(29250..30254)
                     /locus_tag="PLD05_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOP76709.1"
                     /translation="MMNMKNLLVALIFICLPNCALAENWATKMFETLEHDFGVVARGA
                     KAEYRFVFKNLYMEDVHVQSARSSCGCTLVRVENPSLKTYETGAIVATVNSRAFVGAR
                     GATITVVFDQPYYAEVQLRVRSYIRSDIVFDPESVNFGTIDCGSPAERTITIRYSGSR
                     SWRITDVVSPLPYLTAILSEPSILGSETVYRLTVKLAEEAPPGTLADHLILKTNDRTS
                     DAVPLRVEGTIVSPISVTPSTLFLGRMKPGEKVSKQLVVRSKQPIRISKVDCEPCNGV
                     LEADLSRAGNPATVHVIPITYTAGEELGQIVANIRIHTDAPSVNESPLLARAVAVVQK
                     "
BASE COUNT         6609 a         7966 c         8641 g         7102 t
ORIGIN      
        1 ccataaacaa catggacaat gtcggtatcg tcccaaacac cctgtccgtt cagcgtgccg
       61 gcacgtacct gcatgccgtt aatgccgtta cccgtcatgc ggttttcgcg gacgagcggg
      121 ccaaagttac catcatattg cgagaaggca tcgatgaacc ccgtgcttcg gccccagtcc
      181 ggaacctccc aagcattgag ggcgttcacg tcgatgctaa ggacgtgtcc ctgattgttg
      241 atgaacaggt tgttgacaat gaccggttgg gcaccgcgga cataaatagt tgccgggctg
      301 ataaacccgc gcccgttgcg gtcccctgcg gaattggccc cgttatttgc aaagcgcgta
      361 ttggcgatac ggacatgggc ctgccgaatc tcgatcggat taaacccggc gaaaccaccc
      421 tcgatggctg attgaccacc ggcgtaggca ataatcgctt gatcaatgct gccctcggaa
      481 agcggggcaa aagcgatacc cgcccagttc cctggtgctg gttgttgccc acttgtattt
      541 tgggtcgtat tgaacgttcc accggcacca tagcgggtgt caagcacgga ggtgaaaacg
      601 accttgtatc ccggcaccgt ctcctcgaat ccagcccgac cctccgcgat gatctgggca
      661 ccgatctccg tttcgatccg gctgccctgc aatttgacga cgacaccggg atccaccatc
      721 aaacgggcgt cgattcgagc ttcgcgattt cccaggcctt caactttaag ctgagcacca
      781 cgctgttcga gtgtaacggt gtctccgcta ctggtggcgg caacttcaag cccccgagcc
      841 aggcgggctg cattaatcgc agcagccaaa gaagccgcaa tttgttgagc agtgctcgtg
      901 gaggtgtaac ttacgggtac gcgccccgtg accacgccat ccccggcatc aaactcaaag
      961 acaacgcgtg ttcggccatc gaagatggag aaatactggc cgtccagttg cggacttgcc
     1021 gccggcgcgc ggagatgaaa atcatcgacc aaagtcacat caggagccga cgacggtcgc
     1081 atcaagggac cgcccggtgt tccagcgatg attagatttt cagggacata gtgaacaatg
     1141 tcccaatcgt cccaccgggc aaaggttgtc agtttctttg tcgcaccacc ggccggggtc
     1201 ttgatacgca cataaaggcc gttgatggtg ttgtccacga cgtaattgcc gcggatcatt
     1261 gggccaacac ggtcatattc cacggtgtag ggttgattgg gatcccaact ttgataatgc
     1321 gtgtcggcaa agctgttggg atcggccgac atggcggcat cggcgctctt tgtgatacgg
     1381 ctgaagacca ccgtcggccg ggcttcaacc atgtgaacgg gattatagac cgagcgcacg
     1441 ccattgacga tgacttcgcc accgccatac cgtatgtccg catggctcag gtaattcaag
     1501 aaaatgcctt ggtcttcgag gacctggcgt ggcggcaagc ccgaggccgg gtcataagcg
     1561 cggatgaact gataatcgag atcattcttg aagacgatac cgccccaatc gccggagagc
     1621 ggcgtcgtac tgttagtcgg ctcaggatcc cgaccaaggc tttcgtcgta gtaggacgtg
     1681 aagtacaccg agtggaacgg tgtgccgagg acttgcaaag caccgccgct ccggtcgatc
     1741 ccttcggcaa tgcttcccac catgatgttc gaacggaaca gcttgatcat ggccccggcg
     1801 tcgatcataa cggtgacgcc cttgggcacg tccatccgca ccccgtccga tagcggcgca
     1861 ttatcgacca ggcggcggcc gatctcgtac gggatgttgt cctgaagggt ggctgggttg
     1921 ctaggatcgt cgttggcaaa attgttgccc acaatccgca cgatagtgcc gggctgagcg
     1981 acggccaagg cggctgcgat ggtgctgaaa ggattgtcaa tcgaaccagt gggggctgaa
     2041 gaccctgaga gcttatcgac aaagattgtc tgcgactctg gctgcaccgt gaaccagaag
     2101 ttgtaatcac caccggggat gccatccaag tcaccatcga aatagacacc ggtcgaatcg
     2161 atcagccgtg acgggttggc gaggttcggt tcacggaacg tatcgggatt gttcggatct
     2221 acaccgcctg gtttataggt cacgcggagt ttgtattcac cttgtgttac ccccccaatg
     2281 cccgagttgt ccagtgtcgg gtcgtactgt gtgtttccac tggcactgac gccaatgtaa
     2341 taccgccctg ctgggagata aacactgata gccgaatcct cgctgtagaa gtcgtcattc
     2401 cgagccacga cctcatacgt ggtctgcgtg aattctttct ccactgggac gccgttttct
     2461 atcaccacgt acttctgcag cgtctggact tcgcgataaa gagtgatcgt cgcgtcgaga
     2521 aggctcgagt tgttaagtcg ctctgccagg acctcgatgt ccacgagacc gcgctcgttc
     2581 aggtcgaacc ggtacatgtc gatgtccatg ttgtccggcc ggaacatgtg gcgcccgtgg
     2641 atgatgtcgg ccacgccggg gaagaccggc tcaaagctgc cgacagcgct ttgggcgtcc
     2701 tcagacctgc ccatgatgac aaagggctcg aggtcgtaac tgtgaccgta accgaggagg
     2761 tggccaattt cgtgcatggc gacctggaac catccaccgc caaattcgct ggcgccccag
     2821 ttttcggcgt agtccatcag ggccatgccc cctccggcaa caccggccac cccgccaggc
     2881 gcagactgca agcccatcaa agccagatcg cccgtgatga ttgccagccc acccgtttcc
     2941 gcttcgtaga actgaatacc gaggtattga ccgtaaatct caaagatttc tcgcgtccgc
     3001 tgtttttgcg cctcagtgat gaggttgaag cttcccgttg ggaagtagta cgccaccgag
     3061 gggatctgtg tcgaaccatc tgggctagat tcccccccaa aatgatcctc accttgtata
     3121 ctcgggggcg ggtaattcgg cagatcgcga tgccctggct catcgatcgc gccaggccat
     3181 tcgagattat aagcgatcgg cgtcacaatc gatcccgaga tgataacgct ctgtcccagg
     3241 tttccagtgc cgaagccgct aatgaccgtc gcagcgtaaa aactagatgc ggcatcggcc
     3301 ccttctgaca tattccatgt ttggatgggt tgatacgcgc tgccaatgcg gaggcgtgct
     3361 gcgcccgttc cgtaatgcgc aagatcttgc gcaaacacca gccggacaag attcttcgcg
     3421 gcatcgtaag tgaccgacgc tggtgtaacg acaagatcgt cgcttggagt gagtgtttct
     3481 cgagtgaaga tcaactgata gaagcgcgga tcttgagcag catttcgatc aagcttgtcg
     3541 ttgttgaaat agacctcgat gacattccgc tcctgataca ggctcttgac ccgcaatgga
     3601 ctgttctgac gatcgacaga gacctgactt ccacgtaatg tgacaactgc gccgttgacc
     3661 aaaaccgtca ggtttagccc ttgatttaaa atagcggttc caatcttctg cgcaatgagc
     3721 gatgcggaat caccgcttgc atatacgata ggtacattgc ccgcgcgtac accattcccc
     3781 gtgtcgaatt caaaggtgac ctggtaggtt ccatcgccca cacggaacgt ttgaccatcg
     3841 gtgatctggt ttcccgcagg cgcggtaatc tcgaagtcac cacggtaaac gggttgcgga
     3901 acgacggcca acacttgagg tccgacattg atgtcgaact ccatcactag gtcacgacct
     3961 gtcttaaggt cggggtcata acggaatggc aaaggcggcg aatcgagatt cattaacggg
     4021 ttggcacccg tgcctttgat cacaagacgg tacttgtcgt ccggcaggtt ctcggcaaaa
     4081 cggacgatga cttcattcgg tttgtcacca atcccgatat aaccgatcgg gacaacaacc
     4141 tcatttcctt cggcaaagga atcatcacca ccagctctca cgaactgaat ggcgccaagg
     4201 gtccgcgggt caagttgctg tccctcgtta aaccgcagaa tcaactcgcg aggagcaacg
     4261 agtagttcag ctccttcctc gatcaggctc ccttcgttgg ggatcactgc cacgagatcc
     4321 ggcggcgttc caggaacctg ttgtccgtaa acggtcaagc gccaggaatt gagccttccc
     4381 acaacgccac ccactcggtc ccgaacgatc agtgtccaat cgcctcccga cgtttctccc
     4441 cagttacggt ttgttgtaaa cgtccactga acccctgtat cgaatggatt gtgaatgtgt
     4501 ggagtcgcaa ggactgattg tgtaccggaa ggcgagacca aaacaatctc aagatcgcca
     4561 ggacttagat gagtgatatc gaccgtgacc tcaacccatt cgacccggtt gatgtcggaa
     4621 gccatgctaa tcgtgctgga aattccgacc tggctgttat ccggaatgac ttggtttacc
     4681 tggaagatac cggagtccag ggcgatctct ggcagaacag gcttccaatt cctcgcggct
     4741 tgcacggccg ccgctgcgtc gatggcccca aatccgtact tgtggttgat gtgatacccg
     4801 gcagcattcg tcgtccaatc gggatcggag ggatcaacct tgcgagctgt ctgcgcgagg
     4861 atatattgga catcccggta tgtaagattc gggtttgcct gaagcatcaa ggcaatcaca
     4921 ccggaaacgg ccggggctgc gaaagacgtt ccttgaacac ccgtgtaatc gcctgtgctg
     4981 gacccattgg gaccggtcca atctgttgtg ataatccccg acccgacatc attgggatca
     5041 gccatttcac cacccggcgc aacgacaaac aagcacgccc cgggttccga gtaaaaggct
     5101 tgttgaccgt tctcattcag tgcaccgacg gctatgacgt agcgcaggtt tgcaaacccg
     5161 tcgtagttaa cgttgtccaa taggtagccg tcattaccgg cagcgaagac gtagatgttg
     5221 cccaaaccgt ttcggccagt ggtgacaccc tgttgaatgg cggcgagggt caaggggcca
     5281 ggtccggcaa agaccgagaa gccaatgtcg cccggaccag cgggccccca actattgttg
     5341 taaatggaaa taacgtcctg gcggtgcgaa agggcctgcg cctcttgaag atcggttgtg
     5401 gccccggata tgagacggat ggccgcgatc tgggcgtcaa aagctactcc cgccacgccg
     5461 gtggcattgt tacctgccgc ggcggcagta cccgccaccg cggtaccgtg accagtgtcg
     5521 tttggaagtt cgtgttgtgg atcggcatcc cccgcgtaat agtcatagct cagatcggac
     5581 ctgtagttag ggagcaaatc cacgtgtccg gtatcgagac cgtcatcgac gatcccaata
     5641 acaaccccat caccctgata agcgtcccag acggtcacca cgtttaggtc tgcgcccggg
     5701 acaccaccgc tttgaccagt gttcaacaga tgccactgtt gaacaaacag gggatcgtta
     5761 gggatcaagc aaagtgaacg ctgttgagga acaagcgggt agaaatagtt caccagttct
     5821 gaagtttgca gccgttggat ggccgtctgc caatcgacac tgctcgagaa gcgaaaaacg
     5881 tacgcgcctt tgattgttgt gggagtgggc aaatactgcg cgccgaggag cgttgcaacc
     5941 tgtgcggggt tgatatcatc cccaatccct accacccagt ctgtggtaga ctgtaactgg
     6001 gcatcactat acctatcgag gtcggcggct tgttcgatcg cccgctggag tgtgtcctgc
     6061 cacacccgat ccaaagtggc tgggttgatg ctcaaaagga cacgatcttc cattcgctcg
     6121 acgcgcaagt cccgggggcg atgtgtcctc ggcttctttt ccgatttctt acgtgaaccc
     6181 tttgcgctgg gacgatactt tgaggagaaa tcgcgcaagg tgtcccaaag tttcattgtt
     6241 gaatctccct cgagcaatgg atatttgcgg ccgacagccg tatcaaagac aatccctcat
     6301 ttaagcgacg atcacgaaac taacgtatct accgattaaa attggggaac ccgcaagatt
     6361 caacccaaat cctccaggtc tcccactttc gcagtcgttt gtcaattgca attcagcact
     6421 cgacccgtca agtgggcagg gcggctgggt tgccacgctt acgggtgcac cgcccggaca
     6481 caccggtcgg ctaagtgctc cccatgcgca cgataagcgc acagttcgtc gtctttagac
     6541 gatcttgcaa aatcgttagt tccggaaaaa tgggccggtc cgacgccgta ccactccggt
     6601 tcctatccat accacccgtt agacgtatta gtcgtccagc ggctggattc tctggtacag
     6661 gtgccgattt tctgatctgt cggagccaaa gacgcgcttg ggtaggcaga gtcgttggct
     6721 atgacgagcg aacgacatgg ttgacgttcc caactttcgg acgcaatcag ccatttctcc
     6781 gcagccggta accctggaga gttgagtcgg cggcgtggcc gggttaaatt aagcggtgtg
     6841 gaatagtgtt cccaaattgg tggaatttcg ttgacgtggg actgggtcgt tttagaataa
     6901 atgagagaga acgacccacc ggaaatggat ggcttgtcat gaagacacag gttttgatgt
     6961 gggtcggagc gactgtgttt ttgctagcgt gcggcgcgtt tctctgggct gcggcggagc
     7021 cgggtttgat aggtcctagg gtaacccagc cgacgacaca gactccaggc tggatcgtca
     7081 cggcggcagt tggtgacaac cgtcagcaga tcgtaatcgc agatccagcc caacagtcgt
     7141 tggcggtgta ccatgtggac cttacaacag ggcgaattgc cctgaagagt gttcgccacg
     7201 tgcgctatga cctattaatg accgaattta acaccgattc gccttctccg cgggagttgg
     7261 aggccttact caagtctcct tagcgatgcc aatggctggg gaacggtagg gggacaccaa
     7321 accgttatag tgaggtgtga aacaatggcc caaaagtttt acacgacgag ggaagcagcg
     7381 gaactgcttg gaatcgctcc ggcggatttg aattcgatgc gggagcgaaa cgagattcgt
     7441 ggttttcggg acggcatgga ctggaagttc cgagccgagg atatcgaggc cctccgcctg
     7501 aaattgcgtg ccgatgcgac gcgcggtgct ccacatcaga cgccatccag tgacgaagag
     7561 attctcctcc tcgatgacga tctgaagcct ggagatctgg gaacgagtgg ttcggggacg
     7621 gtggttggag gcctttcagg tctcgatgct ggaaagagcg atgtcaaggt agcgggaagt
     7681 gacgttaatc tggccggaag tggtgttgga atctcgggca agccggaaga gtcctcttcc
     7741 agtgtttctc cctttgacga attggcaccg ttggagactc ctgccgagac cggaaaatcc
     7801 ccagtgggta ccggctcgga ttcggccgtg aatctggccg gggagggtct ggaagatgat
     7861 gatgttgtga tcggcgggag cgggagtggc agcgacatta cactcggcag cgatagcggc
     7921 atttcgctga tggatacgaa tgacagtggg atctcgctgg agggtgggtt ggagctcgtc
     7981 ccctcggaag agtcgctcga actggacgag gacgatatgt tgacgttggc agcgggggct
     8041 gataccgaag cgccgacgca actgcggacc gacgaagagt ttatgctaac cccgtttgag
     8101 gaggccgaaa gcgaagaaac cgaaagcggt tctcaagtca tcgcactcga cacagaaccg
     8161 gctacgggtt cctcgcccgg catggccgaa ctcttggggg ctgaagcggc ggcacctttg
     8221 ggtgtggcgc cgacaattcc tggggctgtg cctggagcgg ccgcgcccgg aattggcgca
     8281 tttcccggtc cggtcccggg ggtggcccca gtgatggagc caagcatggt ggttccggtg
     8341 ggcgaggcgc cttgggggac gctgtggaat acgatgctca ttgtgtgcac cgtgttgctc
     8401 gcgctttgtg gcatgatgtg cttcgacctt gtccgcaata tgtggagttg gaatggcccc
     8461 tactccttaa acagtacgat catggatgca attctcagcg tattaccgaa gtgacacccg
     8521 ttagtgcgtc ttgtacctgg cgggcgaggt aaacgcaccg ttttccgcgg tgcgtttttt
     8581 gttgcatctt gaatgtgatg tccgcccttg taacggcgcg tccaagcggt gcctagcctt
     8641 tttcttgccg ctgtctctat aatcagataa gtccattggc agttcgtgtt acagctattt
     8701 atgtgagttg cacgagggct tggcggtaag acctgtcaaa gttcctcatg gccgggcaag
     8761 ctttgatgtt cccctcgggt tacggttccg cgactgtgtg gacctctctg gctcgtgatg
     8821 tgtgaagatc gctgcgacgg gaagaaattc attggaatat tccatctcaa tccttgattc
     8881 gagtagtctg gcatgtctcg tgtacgtctt cgacgacctg accatcctct tccgcttcag
     8941 tttttcctcg cccttatcga gtttctcgcg tcacttcgcc tggctgtcac actgttgact
     9001 ttgctgattc tcgtgttggc cgtggccacg ttcattgaaa cgagctatgg cgctgctgcg
     9061 gctagattcg ccgtgtatcg ggcgtggtgg tttacggtcc tcgggggatt gttggcgatc
     9121 aatatccttt tcgccgcgat tgtgaggttt ccttggaagc gatatcaaac gggttttgtc
     9181 atcacacatg ctgggattct tgtcttgctg gccggtgcgt attggaccgc acttgaggga
     9241 atcgatgccc agctttccat ctatgagggc atgattggcc atatcgctta tcaggatacc
     9301 gttcatttcg agatcgacct cacgaagaag tcgcggagcc caaaccagga taacccctcg
     9361 gctgggtcca ccgaaaatgc ggcggattca aggccttccg agagacgggc gttgcgggta
     9421 gcggtgcctt tccactgtgg gccttttaat tggtgcgatt attcatcggg tttacgacac
     9481 ttgttgtggt cgcctccggg acgattgccc gtcttgccgt ggcaacttgc ctggcgggac
     9541 aaaggagtca tattcacaga gggtgatcta tctttggaag tgctggatta ttacgctgac
     9601 tcgcgacgtg tcaggggcgg ttatctggcc ggctatataa aagcgaatac tgcaacgtcg
     9661 gctcaaggta cgtggtttcg tccctgggaa cctattgagt tagatgttca gtccagacgt
     9721 acggaggctg gcataacgac ggcaattggc tcgcgggtga cgctgagcaa tggcattcgg
     9781 gttgtcttga cggccgcacg ctctcaggcc gagcttgacg gtttcctgct gggaacgccc
     9841 gtcgaacctc ttgatcacga ggggcaagtg gtcctggttc acaatcagat tcgctatgtc
     9901 ctacctgtac gccaattgcg ggcagcaaca ggtgataccc cattggaggg ctcaggttta
     9961 cggatcactc actttgaaaa ggacgtgccc cttcaggccg tgcaattgca gctaaagaac
    10021 gacgaaggtg ttgaagatga agtgatcctt tttgccgatc ttcccgagtt taatcgacaa
    10081 ggacaaaagc tggctgtcgc cggcgcatat tgggtcaatt tccctagtct tctgtccgat
    10141 gtcggacaag cagtacgtct tcatccgcgc gcaaccacgg gggcgacacg ggcacgagtg
    10201 gacctttgtc agggtcctgg tggcaaattg tattaccgaa tttggcagtc gcctcaggtt
    10261 ttgaaaacag gagaaataaa ccgcgatgac cgagaatcga tcctgctcgt cggaaatgat
    10321 ttagatgttc gtttggtagt caacacattt gtcccatggg atccagaaca tccggaggcg
    10381 gagacggttg ttcctttgcc gtttcgtaag ggtgcgcgca tgacccaacc gagggtcaag
    10441 gtgagattat ctgccggggg aaagacgcac gaaacgtggt tggcagtcct cccaagaggg
    10501 gccgaccgac ccatcgatgc cgaagagatt gccacagtgg ctagtgatac cgagcaaatg
    10561 cgagtgactg ttaaatacga tgcggttgat gtgggctttc ggcttaagct aagggaattt
    10621 cagcgcaagc ttgatcccgg tacaaatcag ccgtcgtact acgccagtct ggttgacctc
    10681 tatgtgccgg agcggattgc caaggaacat ggctttgaga atccccggtg gaaagaccaa
    10741 acaatcatcg acccaagcga tggcgaaccg ttgcagttgg tgcatcccag gatcttgatc
    10801 acattaaatc atccggcgaa catcgtggac ccaattacgg gacgcagtta ccgcctttat
    10861 caggagagtt ttagcggtcc gtttcgaccg ggcgatccga tctatgaaca agtcgtgggc
    10921 aaagaaaagg gaccgccgga gctttatctc tcgacattga gcgttaacta tgacccgggg
    10981 cgagggctga aatatgccgg gtcgctgctc atcatcggtg gcatcgtcct gatgtatttg
    11041 atgagggttt acatctttcg cccgcgggta aggccaccgt ttaggaatga acagctcgag
    11101 caaagtgtgg ctgccgggcc ggttagtgtg acgtgaacgt atgaatagta agactcaaga
    11161 acagatccag atacttcgac ccacaacgca aggttaccaa attagcggag cgtgtttcac
    11221 cgaaaaggat tttctttatc tgtttgaatt gttagcggca ataagatggt tttctgtcgg
    11281 gcaaattgcg gacctgtcca tgccagtata cgaagaatcg gttcattgat gacgatggaa
    11341 atgcgcaggt tattattcgc ggttcaccaa tactttcgta ttgtcgtctt ggcttggttg
    11401 tgcccggtcg taatcggaat gtcggcgacg gctggggcgg acgatcggcc catcgattgg
    11461 tcctcgtgga agcgacttcc tctcttcgaa ggtggtcgta ttatgccgct tgataccttt
    11521 gcccgtcgta tcgtggaaaa gctgactgga aagccgcagc cgattcttgg tcctccgatg
    11581 acgcccgatg gccaattgga taggcagcat ccgttgtttc cagatggtct tctgcgaaaa
    11641 tggccagcgg tcgaactgct tttcagttgg ctggctgaat cggaaaagtg ggaggacgtg
    11701 ccgttcctgc aagcgagtca ccaagatttg cgtgagcggc ttctcaaagt gccattggtt
    11761 gatgcatccg ggaaacgcct gagctatgtc tcgccacggg atgtggaaaa ctccatggag
    11821 ttcgaggcct atctccgtga cctcagccag cgacggaggg aagttgagtc ccgaggccaa
    11881 cgcttccgga tgagcggtct ggacctccac gcggcacgat tgtgggacgc atatgccaca
    11941 tttcgcctac tgacatatca gccacgtctt gatagccgtc cctggtttcc acctaacgtc
    12001 aaggtgagcc atcccattcc gcggcgtttg gaatctctaa tcgattcctg gacagagatt
    12061 gagccgcaac ttcaacagct cgcagatttg ggggtatttt catccggtca gtcacagacc
    12121 gcggggccag gtgagccagg ggaagcccct tctggagacc agtctggcag ccaagaacat
    12181 ctcgttgccc gtgcgagggc cgagtttgcc ccgcttctcc gcgctgcgga gtctgaggag
    12241 gtaacagttg cggaactgga tgaggcgctt acccgattgc aaccgctgct ccgcgaaatt
    12301 gccctgtgtg tggaatcgct gcaaaatcga gccttttcag tcgaggcacc cgacgcgacc
    12361 aagcaacttc gtctggacga tctccgcagc aatatccgcc tcctggcaga aaagtcgcgg
    12421 cgtctggcgg acgaggtcga ggtctgcgtt ccgatgttgt acgataccgg cgttggcccc
    12481 cacgtgttgc cggcgatgat ccccgcttgt cttaattcgg caacgaacgg tgacgagccg
    12541 gtatccccgt ggctaagttt gggcgccata ctctatggtt caaagaatgt gctccgaggg
    12601 tatgacattg accgggtgaa ccaggtccgc agctttttcc agatggccgc cgcggcatat
    12661 gccgaccgtc gatcgcacga tcgaacgcgc cgcgtagaag agaatctcga tcagttctcc
    12721 gcagcgttgg ctaagttagg acgatccatt gaagcggaac ggcaacgttt gccggtggat
    12781 gagtctcatc gagaattgtt ggctttgact gcttatccgc cagcagggtt taccgattgg
    12841 gaagtatttt acaacgagtt tgacccattt cgctggtctt ggatcatcaa tcttttggcg
    12901 tttggcatat tgctccttgg cgtgggacgt ctccgtcagc cttctttgtg gatcggcgta
    12961 cttgttctta ctgtggccca agtggtgacg atctacgggt ttggccttcg cgtggcgatc
    13021 tctggctggg cgcctgtgac caatatgttt gagtcggttg tattcacgtc gctagtgatt
    13081 ggtctgctcg gcgtgtggtt tgttatccgg ccgttggtgg gcaaagggat tcgcgagggc
    13141 tgggagtggg tgtctctgcc gcatgagccg ttgaccggca aaagggaagc gaggacttgg
    13201 caggacaagg ggacctttgt aaacgtgctt cggggagctg gcgtcctcgc acgattggct
    13261 ttaggcgggc tcctgtttta ctatctcgcg gtcgttccct atgatgtggg caagagtcgg
    13321 gccgtgttcg atttggtgcc ggacatcacg cggtctttgg gaagtccgac tttctcctca
    13381 atcgtgatga acctcttggg gtggtttgtg ggaatgtgcg ttctcctgtc cgtcgtgtgg
    13441 ctcgtgcctc gctttgccgt gggaatgatt ttgtcgcttc tctggacgat tccagtggac
    13501 ttgaagaccg cagaactggg accgcgcctc aacgaagtgg tgcttcggcg accgttcgct
    13561 gtggcagccg ccggattggc ctttttggca gcagtcgttg cctattatac ccctgtgtgg
    13621 gacaagggga tcgagcccct gcagccggtc cttcgtgatc ggctgtggct ttttgcccat
    13681 gtcctgacga tcacggcagg atatggtgct gggttactcg catgggggtt gggaaacatc
    13741 tcgttgcttt atttcctctt tggccgttat caggctgtgc cccgaggcga gataatagta
    13801 cacggtgagg cacaccgatc agtggatgga ggtggacgcg atgatgggtt gactatttct
    13861 ccaccgttgt catgctttac gctggcaacc tacatgtaca aagccattca attcgccgtg
    13921 atccttctca cagccggaac aatcttgggg ggtgtttggg cagatcgggc gtggggtcga
    13981 ttttggggat gggaccccaa ggaagtttgg gccctgatct ccattctcgt ttatatggcc
    14041 cttttgcacg ggcggtacat cgggtgggtg aatacctttg gtttggcagc cggatcggtc
    14101 tttggcatga tttccgtcgt ttgggcctgg tatggcacca actacctaat gcccgcgggc
    14161 ttgcacgcct acgccgggga aggtggtgga ggcggtttgt acgttgtcgg ggccttcacg
    14221 gccaatttgc tttttgtcgg tctcgcctgg atgcggtatc gtgcggaaat ggcgaagggg
    14281 caaggagccg tggacgctcg aagaggtgaa gagactgagg ggcatcctga tgaaagcggc
    14341 atcgcctcga cctctgtttc tgggcagtaa ggacagttta ggccttgctt ggttggctac
    14401 ctacttttcg taaaacgaga ggatgctttt gcgggtcggt cgggtgaagc tcgattacat
    14461 tccccgatcg gtgtgagacg gtgccagagt gggcaaaacg cccaccgagg cggatgcgtc
    14521 ggtgccattt ttcgggccac caagtacgga atccacagag acgcaccgta gcaaaaactg
    14581 cacaaaggcc tgctgccacc ggtgatcagg ccatggctct tgctgagggg gcgtcaaatt
    14641 gttcaagatt tggccccacg tgacccgctt gccaagggtt tcttccgaaa cggtgacgcc
    14701 ccagcctgct ccagattcca gcaagaccca actgagcttg ccgccatcgg ccggcataag
    14761 tcttaccccg gtttcgtcgc aatcaaacct caagaccaaa tgttgaaagg ggacatcgtc
    14821 tccttggagt atccccgcaa gctggtctgt ctctctgaaa ggatgggcac cttgctgatt
    14881 tgcctgcatt aacgcaaggt cactcggcac ttcgaaaggg agaggcttgc cgggatgaag
    14941 tgatcccagc ggccaacttg ggtcccctcg ttgcatcatc gaagactggt ttccctcata
    15001 tgcctgtacc gaagtgtgga atgagtcgtg aaggaacgta agaaaggcac gtttgatttg
    15061 tcccgggcca gcatgcacag taatatcagc cgatttgagg gttccattta tgaagaccac
    15121 cctgtttaag agcacggaac cctccgcgtc caggcaatcg aatagcgatg gtgatgactg
    15181 ggtggcttct tcccggctcc aaagaagttt tccagaaatg gtcgcggacc agcaagacag
    15241 ggactcacag gagtgactaa cagtcgcctc aattgttccg gcccatcgaa ccgacgatgg
    15301 tatttcgtgc agcccgttcc acaagggttt tagaaccacc agaggaatgc ccggttcctc
    15361 cactcttacc tgttttgtca ggtagaactg cccatccttg catgttgatt gccataccat
    15421 acttagcgtg ctttgcaatg gaccgtcgga cgggcgcaag tcgggcaacg gcggctgccg
    15481 ctggtcttct gccgaacaga caattcgaca tttgatagca cgctggcggg acggcgatga
    15541 aaactcggtt tggagtgacg aacataacac aagaggttga ggatttggtc tggcttcatc
    15601 gagtttgggg acaacttgga tttcactcgc tgtgagtgtg aatgtctctg cggcaaaatc
    15661 acgtttgccc aataaccatt cttcacagat ttcccaaagg cgtaaccatg cttcttcact
    15721 caaagaaacg ccggaggcgt caatatgcgg tttgttgctt tttcttgtgg acaacgcgtt
    15781 ttcaatcacg caaaccgggc tccccgtgag ggtattgcgg agagtcaggt gtgagtatcg
    15841 tttcacgccg ggtcgaggtg tttgatagcg ttcgatagca cacgagaagc ctatagcatg
    15901 tgctattctg tcgcactcgg tacgaatgcc catgcctggc cgccaccaga gggtaaaacc
    15961 gaggatcagc gcgaggggga cagtccatat caggagcagg attccaatca gcatcacttt
    16021 tgctaccgaa tcccgcatgc gcatgacctc cgtgacgccg gtgcgattct cctgtgtaga
    16081 aaggcccttt ggaaaggtgc ccgaacagca agcgtagccg ctgccacacg gtcccaattc
    16141 ccggcagctg cttctagaaa tgctgcgcgg ttacaatcct agcctttccc ggttcatttg
    16201 ctcaaaaagc tttatacgct ggcggatttc ctcgatgtgg tccccgccga atttgtcgac
    16261 gagagccgcc gcaatttcaa aagccacgac attttccacg attacgctgg ctgctgagat
    16321 ggcacatacg tcgctccgtt cataggcggc cgtctcgggt tgcttggtct ctaaattaac
    16381 ggactccagc gggcgtgcca gtgtgctaat cggtttcatg gccgccctca acacaatagg
    16441 ctgggcgtta ctcatgcctg cctcgaggcc ccccgcatga ttagtggcgc gcacaaaacc
    16501 aagcgtccgg ctgtttacct gcgaaggatc aaaatggata gggtcatgaa cttgtgaccc
    16561 tggccgtcgg gcggcctcga atcccaggcc aatttcgacg cccttaatag cctgaatagc
    16621 cattacagcc tgtgccaacc gcccgtccaa ttttcggtcc cactgggcat gggtccccaa
    16681 tccaaagggt acattttcga cacgaacttc gaccacgccg cccaccgtgt caccctcttt
    16741 ttggcatttc ctgatgtagt ccttggcctc ctcttcacct tccgggtgta gcgtgtaaag
    16801 ttcggacgaa tcccgaagtt gtcgtaacat atcgagatgg ccttccttgg ccgggaattt
    16861 gatcggtcca acctcgacaa cgtatcccaa aaggtcaatc tgacaagcac taagtaattg
    16921 ccgggccaaa gcccccgcgg ccacacgtcc aactgtttcc cgtgcacttg cccgctcgag
    16981 aatcccgcga atggatgaga aatacttgac agcgccggcc aagtctccat gcccgggacg
    17041 gggtcgtgta aggtcgtcca ttttctcgat tttgtaatcg cgattagcca cccagatagc
    17101 aatggggctt ccaatcgtga cccctcgcca ccagccactg aggatttctg cttggtcttg
    17161 ttcaattcgt tggcgtcccc cgcgaccgaa tccgccttgt cgccgaagta attcgcgatt
    17221 gatgatctcc agattgagtt ctaccccggc gggaaaccca tcaacaaggg caagaatccc
    17281 cttaccatgt gattcaccgg ctgtccaata ccgtagcata actctttttg tcctcgatat
    17341 ttgggcaatt cgcgttgagc tttgagttca aaagtactgt aattgcaagg atttttcttt
    17401 tgcattcccc acattgtatc gccctatgga aggttgtccc agagcgaatg accgttcctc
    17461 actgcacagc acacgacggc gcaggtcgct tcaagcgttt ttgtgccgga gggtcacgcg
    17521 ccaccgcgat cagaacgaat aattcccgcg agggttggac acggctttac cgcacaaatc
    17581 agtggcagcc acaacggacg tgtcgcatgc agttgggaca aatttgagcg cttcgttaag
    17641 acagcacacc ttttgtgctc gggcatatgg ttgagcctta cgggttctcg tacactggtt
    17701 agcggcagaa atcggatggt gtgtcggtca tggcaagccg acgtcctggt tggacggaaa
    17761 ctatactgag cggaggttca atcatgagag ctagtcaggc agcgatgctg gcgaacgtcg
    17821 ttagggacgt gttttattgg gcatgtttct ccatcttcat gaccagtgtt atcagccaag
    17881 tcggtgtttc tttggcctcc gagccgggaa tcggccagat tgagacgttg ccgggcactg
    17941 agcctctcga ctggtcgggc gacatagcct cgcggatgat cgacgcgatc gacgcattcc
    18001 tccttcgcca gactgagcac accgctaaat tgcgacaaca ggcctgggca tccgtatttt
    18061 cccccgatca atggccaagc gactggctcc aggcaaagcg ggagcgtctc gctcaaattc
    18121 tcggcgtgaa ggatccgcgg gtctcgcctg tgcagatgac catccttagc cccccaggac
    18181 aatcccggcc cttggcggaa ggggcgggct acgatgttta tgccgtgagt tgggacgtgt
    18241 ttacggacct gaaggccgaa ggaatccttc tctatcccaa acatgaccaa cccaaggcgt
    18301 gtgtcgtggc gattcccgat gccgatgtat cgcccgaggc cttgatcggc ctggaactcg
    18361 gtgtcccacc tgcttcgcaa tatgctcggc ggcttgcgga aagcggatgt gtggttgtga
    18421 ttccgagctt gatttcgagg gaaatcagca aacggggcaa tgtgtcattg acgcaccgtg
    18481 agttcattta tcgcccagct tatgagctgg gacggcacat cattggctat gaactggcga
    18541 aaattctggc tgcgattgac tactttgata gtgcttttcc taccttgggt gggaacttcg
    18601 gagtgatcgg ttggggcgaa gggggaatgc tcgccttgta cgccggtgct ctcgatgccc
    18661 gtcttggggt tgtgggagtg agtggctatt ttggaccgcg cgaacatttg tggcaggcac
    18721 ctgtggatcg taatgtgttc gggttgctaa aagacttttc ggatgccgag atcagtcttc
    18781 tcatctaccc acgatcttgt gtaatcgagg cagctcgagg gccagaggtg acaattccgc
    18841 ccaaacttgg tggcgcaccc ggagagttaa agaccccatc gttggaagag gtcaaaaacg
    18901 agtttgaccg ggcaaaccgc atccttcaac aggcagggaa gtcttttcat ctgtcgctgg
    18961 tgccgagcga aaatggttct gggccgtatg gtaccgaacc atttctccgt gcttttcttg
    19021 cctcatttgg tacacccatt actttatcag ccgaagccga ccccccgcgg agaattcggg
    19081 acgatccgtg gcgagcgacc cgcactgagc gtgccattgc cgagttggac cgacatatcc
    19141 aacggttact cgtcgaatcc ccggaaattc gaaaacgctt ttgggccaaa ctcgacacga
    19201 gttccctcga gcggttcagc cagtcggtgg aatggtatcg aaaatacttt tatgacgaga
    19261 tcatcgggag gtttgacatc cccttggcag atgcgaaacc gcgaactcgc aaagtctatg
    19321 ataccctcag ttgggaaggg tttgaggtgg ttctatccgt gtttgatgag gtgtttgcgt
    19381 acgggattct ccttattcca aaaggaataa aaccgggtga acgccgcccc gttgtggttt
    19441 gccagcacgg attggagggt cgcccccaag agaccatcga aggcgatcac cgcgcgtatc
    19501 acaattttgc cgcgcgcctc gctgatgagg gatttgtcgt ttttgcgccg caaaacccgt
    19561 atatcttcgg ggatcgtttt cgcacgctgc aaaggaaagc caaccccctg ggaaaaacat
    19621 tgttttctat catcgtgccc caacatcagc aaattgtgaa ttggttgagc acgttggagt
    19681 ttgtcgatcc aggccgcatt gcattctatg ggttatccta cgggggcaag acggccatgc
    19741 gcgtgccgcc cttggtaccg cagtattgtg ccgtaatttg ctcggccgat ttcaacgaat
    19801 gggtctggaa gaacgcatcg acgaaaagcc gatatagtta cgtctggacg ggcgagtacg
    19861 agatttttga gttcgcattg ggcgagacgt ttaattatgc ggagatggct acgctgatcg
    19921 caccgcggcc gtttatggtc gagcgcggcc actttgacgg tgtggccccc gacgaaacgg
    19981 ttgcctacga gtatgctaag gtccgttttc tttacaacgc actcttgaag ataggggata
    20041 ggacggaaat cgaatggttc gccggacccc atgagattca cggagtcgga acttttcaat
    20101 ttctgcacaa gaacttgaga ttctagaaga cctggcggga agacaaagag cctatcccgt
    20161 aatctcccct caaccgttcg gtgggagagg ggtcgggggt gagggtggct cgaatcgctg
    20221 ccggttttct caattgattg cgacgttcgg ttgccctcac cccaaccctc tcccggaggg
    20281 agagggggac tttttggtag actctaaaga cgagaaaagc acgcagatag gcgagagggg
    20341 actgcttaat ggattggcga agttccgggt aaaagagtgt cgcacttatg cccagagcca
    20401 taagcttttt gtcaattgtc gcaatctcgg gcgaggactc taaaagctac acttcgggta
    20461 agcgacgcgt tgtacgatct ttgacgttcg tgccgacaaa ataggcaata tggataggat
    20521 cggtcgggac tgatagaaag gggatatggc ccgctggttc ggccatgacc aggctgggcg
    20581 ggtgatggca gcattaagaa agctttccct ttaagcaaaa ggtacatatt tggaaaatct
    20641 tgcctcttcg gatttctggg ccttcgctta tctctttttg cagggatgat ctggtcaaat
    20701 tcggtatgtc gaagaaacaa cagaactccg cgaggataga atggacgggt ggccgaactg
    20761 gctgccggca gcctgataaa agcggtggat tttcgcgggt tcctatctga aggtttcact
    20821 cagcatggac cgaactcacc gtttgctcgt atcgcaggtg gcagtgccat atcttcgacg
    20881 gcacctgcgc agactttcga gggccctgcg gggtgtgcct tcggccaagg accccgaggc
    20941 tgttcatcaa acacgggttg cctgtcggcg gttacgggca gccctccgag tatttcgaaa
    21001 cgatatcggc aagagaacaa ccaggcgctg gcgcaaggtt ttccgaaagt tagggcgtgt
    21061 ttttggtgct gcccgtgatc tggacgtaga aatcgcctcc atgctgggcc agatgggtca
    21121 ggtgagggag gagaatattc tccacggtct cgccttttgg ctcgcacacc tcgaggaaca
    21181 gcggcatcga aagcagcctg agttgattcg agcgattcgc aaattccgca ggaaggcagc
    21241 gatcctagaa atgggccaat ggatcaaaag gcagtcccga gagatggtag ccgcgggttc
    21301 ctcgcagggg ggattgcccg tgtcgatcac gaaacagcat cgcaagcttc tgtcggagcg
    21361 attggcggac cttttgcgaa cggcccagtc gctcgaggat ccgcaggata ttgaaggtca
    21421 ccacgccatg cggatcgcgg caaagaaact acggtactcg atggaggcac tggccccgtg
    21481 cctgggtgtg attggggaag tgaccattga agcgcttcgc agtcttcagt cacatctggg
    21541 cgaaatccac gattacgacg tgtgggttga gcgaatccgt caagtgctag atatgatttc
    21601 tggcgggaaa tttgatggcc caaggtggct taatcccgat cgttttatgc cgggcctggt
    21661 ttttttgcaa aacaagtacc aggaacttcg cgaggtccgg ttccgtcagc tccagaattt
    21721 gtggcatgat gaaaagattc agggattgtg gcaacggctt caggcctctg gttttcaagc
    21781 catctcggag gacaatgcaa agtatgcgga tggtgcgaga aaccgtgagg accacccgga
    21841 tgtgaagacc caacatccct gaagaagtga atgtcacttt ttgagcaggt tttctcaatg
    21901 gcttgtcgcg aattagaaaa gccaaccgaa aaaacgtttg tatgaaaaat cgttgttatg
    21961 tcggaccttg ttgatagtca catgacgaca aacgagggcg aagacaccgt ccacccggtg
    22021 gcggtgatcg acatcgggac gaatgcaatt cgcatggtga tcgcccaggt ttttccttcc
    22081 ggggagtttg aggtcctgga acggttgcag cgacccatca ggctggggaa ggataccttt
    22141 tcacgtggga gaattggtgc ccaaagtatg agatcggtgg tggctatcct ccgcgactat
    22201 caagcggtta tgaatagcta cggggtgcag caagtaaggg cggtagctac cagttcgcta
    22261 cgtgacgccg caaatgccga caatgtgctg gatcgcattt atctggcttg ccggttgaat
    22321 gtagagatca tcggaatcgc ggaagagggg cgactgaccg tggccgctgt tcgcgaagtg
    22381 gtagaagacc tcccagaagt caaccaaggg aaaaccctca ttgtggatgt cggcggcggg
    22441 agcacgatcc tgacgatgct ccaggagggt gagattgtca attccatcgg cttaagattc
    22501 ggctcggtac gaattctcga aatgttgtct ttacaatcgg agcctcccga acgtgttgcc
    22561 gaggttttgc agcaacatat tcgcacgcaa atcgccaatg tcgaacgcag tctgaccctt
    22621 caagattgcg acacctttgt ggcagtaggt ggagatgttc gctttgcagc gagagtagtg
    22681 ggtcacccca cgcgttcgga gaagctcttc gtagtggaaa aggaccagtt ccgttcgcta
    22741 cttcggcagt gtagccgtca tactcccaat cagcttgccc acaaatacaa tctgccgttt
    22801 atcgacgccg agacattgct acccgctctt atgacatatt gggaactgct gtgtcgcacc
    22861 tcagcggaaa gagtcattgt gtcgcaggtc tcgatgcgag atgggttact tctggaactg
    22921 gcccgagagg cgacaggccg gaaggacaac gcccttcaag aggggattgt tcgggcggcg
    22981 gaggcggtcg gtcagcgcta tcgggtggac ctcgatcatg cccgtctggt tgcgggcatc
    23041 gcgatcaaac tgttcgattt cctggttgcc gaccatggcc tgggacctcg gcaacgtctg
    23101 cttcttcaag tggctgcctt gcttcatgaa attggcggtt tcatcagcgc cagtgcccat
    23161 cacaaacaca gttattacat tatctccaat accgagattt ttggtctaaa tcgtcttgag
    23221 acgcaaattg ttgcccatgt ggcgcgttac catcgccgca gtccacccaa gccgtcccac
    23281 ctcgattaca tggcgctttc ccgggaacaa cgcgtcattg tgaacaagtt ggcggcgatt
    23341 ttgcgcatcg ctgatgccct ggcgcgcgga aatgttcgtc aagcggagga tgtcgagttc
    23401 catcacgagg ccgacgaatt gatcattacg ctacacaaca tcgagaatct cgtttttcag
    23461 aagcgggcca ttgctgtcaa aagtgacatg tttgaagatg tctatggtat gaaagttcga
    23521 ctgcaagaat gacgttcgat ctcaacgaag taatttgttg tataggcctt ggcttaaagc
    23581 ctatcccata gtctcccctc tctcgtccag cgagagaggg gtcgggggtg agggtggctc
    23641 aaatcgctgc cggttttctc aatcgattct gacgttcgcc tgccctcacc ctagccctct
    23701 cccctaggga gagggggctt ttttgggata agctcttagc aaaaaagttt cctttttgca
    23761 gatcgaaatg ggtaaaaaaa ggataagaat gaaaaaacat accttatcat cgaatcctga
    23821 attgttcatc aaccgggaac ttagctggtt ggaatttaac gagcgagtcc tgcaggaagg
    23881 cctctcggat agtgtcccgc ttcttgatcg cctcaagttc ctagcgattg tggggacgaa
    23941 cctcgacgag ttcttcatga tccgggtggc tagtctcaaa cagttgaaaa aggctggcaa
    24001 gacgcgtcgt gatgtggcag gaatgacacc ggacgagcag ttggcggcta ttggtgcgcg
    24061 ggtccacaag atggttgcgg atcacgggcg gggtattcgt gaggtgtttg gcaaattaaa
    24121 cgaaaaagga atccaggttc tcgcacccga ggagtggtca gacaatgatc gtcggcaatt
    24181 gcgggcctac ttcgaccgtg aactgacgcc gacgctgaca cccttggcca tgagcgatct
    24241 tgttccggct cccgccttgg cgggtcttca gatccatctc gggattgtct tgctgccaac
    24301 gcaacataaa aaggacgagg tgaagatcgc ggtcgtgccg gtgccgcaga agacgccgcg
    24361 ttttgttgcg ttaccgtcag aaggaaaaca acgatttgcc ttggtcgaag atgtgattgc
    24421 ggcgtttgtc ggcgagctct ttaggggtta ccgaatagcc catgtttcac cttttcgtat
    24481 cacgcgggat gctgatgtct ccatccagga tgatgacgcc agcgacttgc tcaatacggt
    24541 cgaggaagcg gttcttgctc ggcgccggcg agccgcggtg cggttggaaa ttcgcccacc
    24601 agtggatcag cggctttatg attttctcgt cagttcactc cagttggagg agaacgacgt
    24661 ttacctggtt gatgggttca tggggactag tttcctctac gagattgtca atctgccggg
    24721 gtttgaccat ctgcgggaac cagaatggcc accccaacct gtgcgtgatc tgcagggctg
    24781 ggacgacctc tgggatgcga tcacggatca cgatgtgatg ctcttccacc catacgagac
    24841 gttttcacct gtcatcgaat ttgtcaaaca agcggctacg gatcccagtg tccttgcgat
    24901 taagcagaca ctctatcgaa ccagtggtga ttcgccaatt gtggaggccc tggaacatgc
    24961 gggtcgcaac ggcaaggaag tgaccgtgct ggtggaagtg aaggcccgtt ttgacgaaga
    25021 gcgcaacgtc caatgggccc gtcgtttgga ggatgcgggg tgccatgtca tttatggagt
    25081 ggcggggctc aaaacccatg ctaaggccgc actcgtcgta agaagggaac atggccgaat
    25141 ccggcgctat gtacatctca gcacgggtaa ctacaacgac aagacagccc gcatctactc
    25201 ggacatcgga ctaatgacgt gtcacccgga gatcacagcg gacatctcca gcttttttaa
    25261 ccttctcaca ggatactcag aagccgtggg atggcagcgg ctcacggtag aaccaggcca
    25321 actcaaggaa cggttcatta gtctcatcga acgagaggcc gaagtggcga ccgccgatca
    25381 tcccggcctg atcatggcaa aagtcaattc actccaggac aaagatatct gtgtggcact
    25441 gtaccgggcc agtcaggcag gggtaaaaat taaactcaac attcgcggga tttgctgcct
    25501 tcgtcccggt atcaagggag tttccgaaaa cattgaggtt cgttcgatcg tggatcggtt
    25561 cctggaacat gcccgaatct tttactttgc caatggtggc aacgaagaag tgtatctctc
    25621 gagcgcggac tggatggagc gaaacctcga aaggcgcctg gagcttgtgt ttccggtagt
    25681 cgatcccagg ttacaacggc gactgatcga gatcttgcat acctattttg ccgacaatgt
    25741 caaagcgtgg gagttacttc cggatggtac ttaccgtcgc gtctcaggcc atggcccgag
    25801 ggttcgcgcg caagaggtct tgtatcgtca aatcgtggag gtgagccggg cttcgccgcg
    25861 ggaattgcgg taccgtccac tgaaaagtga ccgcgccgcc gaagagcaaa ggcaatctga
    25921 taccaaatcg agatagtccg atcaattacg tgcggttttc ggaagtggaa ggcgtcgcac
    25981 cacccaatcg gaaaaaggcg gtctttcgag actttgatcg ctgataggaa cccacggcct
    26041 gggccgtagg aagggcaagc acgctgatac gccgcttttc aaaatattgg aagaatggtg
    26101 tgacatccca cgtgtcgcca tcgggccctg ttccaaggat cacgagctcg gtaccgccac
    26161 gacaaagagt ttcggcaaga tctgccgttg acgatccgct attgggtctg tcgcctttct
    26221 tctcgggcgg ccatttcttc actcggccat cactcagaat caccacatca tttgtgaatg
    26281 tctttttatc gactttaagc gtatcgcgag aggtcatttt gacttgtgga tattccacat
    26341 ttctcaccaa cttgaagaca ttacatccca cggccctggc ggcagccaca accccgccca
    26401 acacaacggc gtcgtcgccc agcgcggaaa ggcaaatacc cccacgttgc tgggcacccg
    26461 gcagttgatc agcatcaata attcgcttga tggtctccca catgtaatcc ccgcaggcct
    26521 cgaccactcc ccctcccaga acgatccatt cgggatccaa aagatgtctc acggtcaggc
    26581 aggcgtaccc gatcacttcg gaagcacgcg agatgactct tgtcaccagt gggtcttttt
    26641 gatccaaagc catccgcaaa acgcggctct taatgacgct gagatcgccc tggagaaggt
    26701 cggtaaggat ggttgattcg ccacgcgcga cggcctctcg aatctccctt tcaatggcat
    26761 ttcgtccagc gagggcctca aaacagccac ggttaccaca accgcacacg ggaccaccca
    26821 tccccatgac gatatgcccg atctcgcctg ccacctcacg ggcgccccgc cacaatttgc
    26881 gcttgcgaac aaatcctgag ccaatcccgg ttcccacgaa gattccaaac acactactgg
    26941 cttcgcggcc tgcacccagc caagcctcgc ccaaagtgcc gaaattgcag tcgttaccca
    27001 gaacgaccct cttgccgaat ttcgcagtga gatgcggttc cagcgacaca ccggaaagac
    27061 aactattggg tgtccgtagg atttcgccac gtttgggatt caccacaccg gggatggcca
    27121 cgccaatcgc ggatatctct gaaaagtaga ggtccgattt tctcacaacg gtatcaatcg
    27181 cgctttcgat ggaggcaatg acatcttcgg gcttgccgct gcgaggcgtt tcccgacgat
    27241 ctcggctgcg gatcacgccg caagggtcgc acacaacggc caagatcttc gttgccccga
    27301 catccactgc tacgaaatga tgttttttcc cgtttttctt tcccatattt cgtctccacc
    27361 aagaggcggt atcgatatgg ccgagccatc atgcgtgttg gctagcccat ttacaatctt
    27421 agcccatatc gctaaacatt cattatgcca gataacgcga gagaataggc agatggtgcg
    27481 ggtctcgggc gatgagccat ccatttcttt cgatgcgaag cagttaggat ggctcacgca
    27541 gtccgcggtt tggtgactaa cctcagggag gaatatcccc tgcttttaac ctacgacagg
    27601 ccgggaggtg tttcatgtgg tgttccaggg caaacaagcg cgtccagtcg gtaattggtc
    27661 aaggagtgtt ggtatcgttt ctcgttacgg ttaccgtggt cgtctccgcc acaagggcct
    27721 ttgcccaagg caaagctgac aatctttggc aattatgtcg ggataaagcc gcgattcatc
    27781 ggttctccac gttcttcacg gcacaaaatg tccgcgatta tttggcaact gatgaggggc
    27841 gtcagtcggc catcgcatgg tgccgccaga ccggcgttac gcgggtcttc attgaatcat
    27901 tccgtgatgg ttacttggta cccaaagacc tgttggctca agcgagtcat gtcttccgtg
    27961 ctgccggatt tgatgtctcc gggtgcgtga cgacaacaaa cgtgggaaag ccttccacgg
    28021 gttggcagat catttcctgt tacactgacc gagccaccca ggataaactg gccgaaatct
    28081 tcaaatacac ggcgtcgctc ttcgatgaga ttatgattga tgattttctc ttttgcgatt
    28141 gtcagtgtgc agaatgtgac gctgctcgaa agagtcggac ggttaacata ggcgagcggt
    28201 catttcccgt ggattctgac acatggtcgg cttaccgctg cgaattgatg gtccaggtat
    28261 cgcgccagct tattttagaa gcgtcccgaa gcgtcaatcc caacgttcgg ataattatca
    28321 agtatcccca gtggtacgat caatttcatg agcgcggtta cgacgtggta aggcaaacgg
    28381 ccgattttga cctcatttgg gttggtacag aaactcggga ctacgacaat ccacggtggg
    28441 gacgcaaggt ccagtatgag gcctatttta tcatgcggtg gttgggcggt atcggtggcg
    28501 agaagtgtgg tggcggatgg tttgatcctt tcgggacgac ggaacatacg tatttggagc
    28561 aagctcgaca aaccgtcttg gcgggggcaa aggagtcgat gcttttctgt tacggggccc
    28621 ttcagcggga tacaggtccc cgaaatgtcg aggtgttacg acagcatatc ccggacctcc
    28681 tccgtacagc agagcaagtg cgtcgccgct cgatcatagg catcgcggcc tacaagccgc
    28741 ctcatagttc acctggcaaa gaagcctacg ttttcgattt tgtgggaatg ctgggattgc
    28801 ccctcgtccc gtgccacgag tttcccaggg aggccaaggc agcgtttttc tcagttcacg
    28861 cgctgaccga tccgcgattt gaggtggaac tatcccgact tgttgagcgc ggtgtcccta
    28921 tccttttgac agatggtctg gcaaggcaac tgaaaaacca agagctgctc aaaaagtcga
    28981 acgtccacgt gttgcaggtt caaggggatc cgcaccgctt attgaagttc tcggaggacg
    29041 aagtcaacca acttcgccaa gcgatgttag gcccttggtc gataacggtt cgtggaccga
    29101 ataagctggg cgtttacatc tttgacgacg gtagctatgt gctagagaac tttaacgatc
    29161 aagcaatccg cgtggaattc aatggcgtcc agcacgacat ccctgcccgg gattgggtcc
    29221 aagtctggaa ataggtttaa agcgaatcac tacttttgga ccacggcgac ggcccttgcc
    29281 aggagtgggg attcattaac ggagggggcg tccgtatgaa tccggatgtt ggccacaatc
    29341 tggcccaact cctcaccggc ggtatatgtg atagggatga catgcacggt ggccggattg
    29401 ccggcacgtg agagatcggc ctccagaacg ccgttgcacg gttcgcaatc gaccttcgaa
    29461 atgcgaatcg gttgttttga acgcacaacc agttgtttgc tgactttttc gccgggtttc
    29521 attcgtccga gaaagagggt gctgggtgtc acagaaatgg gcgagacaat ggtcccttca
    29581 actcgcaagg ggaccgcatc tgacgtccga tcgtttgtct tcagaatcag gtggtcggcc
    29641 agcgtacccg gcggtgcctc ttctgccaac ttcacggtga gacgataaac tgtctccgaa
    29701 ccaagaatcg atggttcgga cagtatagcc gtcaaatatg gtaatggcga aacgacatcg
    29761 gtgatccgcc aacttcggct tccagagtac ctgatggtga tggtccgctc ggcgggactt
    29821 ccacagtcaa tggttccaaa attgaccgat tccgggtcaa aaacaatatc gctccggatg
    29881 tagctcctga cccgcagttg aacttccgca tagtagggtt ggtcaaaaac aacagtgatc
    29941 gtagcaccac gggccccaac gaacgctcgg gagttgacgg ttgccacaat ggcgccggtc
    30001 tcgtaagtct tgagagaagg attctcgaca cggaccaacg tgcagccaca cgaggatcgg
    30061 gcactctgga cgtggacgtc ctccatgtag agattcttga aaacgaagcg gtactccgct
    30121 ttggccccgc gagcaaccac tccaaagtca tgctcgaggg tttcaaacat tttggtcgcc
    30181 caattctccg caagggcgca attaggcaag cagatgaata tcagcgcgac aaggaggttc
    30241 ttcatgttca tcatgaacgt gtcctttcgg agggctaatt caattatacg ccagtgaaag
    30301 tttctcgccg gggcttag
//
LOCUS       DAOSUT010000031        29686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1055_length_29686_cov_9.099794, whole genome shotgun sequence.
ACCESSION   DAOSUT010000031 DAOSUT010000000
VERSION     DAOSUT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 29686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29686
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_29686_cov_9.099794"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1634)
                     /locus_tag="PLD05_04425"
     CDS             complement(66..1634)
                     /locus_tag="PLD05_04425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP76710.1"
                     /translation="MVRSDAPVPPGNSESQATEPDDRGTTGRTRGLWGFLSRLFSRTP
                     EGIESTLEWLMRVSEKWEEKAAPSPSVIVAAGLVMEVMMHFGYREAAWRIGRWLQERQ
                     LPDGSLSESRAAEPVFSATAAAARGWLAALPELNQFEGAAQKACVFLRQWVGEEGRLL
                     PPRNRAWNYEPTTLFNEPPDLSPLLWAGRRWPDTDWTTVVLRAIDYWRHHGHRRVPAE
                     QSSAIVRRAIFFLDWGHRQDAIPLVEKLDRRQTAAGAVPEQDNGSVILLATVAQAALL
                     WFRMFNETRGEQAYRFVEKRRLPDGSFPRSARGTSENDREPDRLAAKYFLEATLWRVR
                     CAYRGRIKNEEPLDAEDPRLLAVRSLVATMPQGSSLADLGCGTGRYLKFLSNWFPHHQ
                     WFGIDFVPEALRQVPRGIKTIEGNLLRIPVSDQSFHAVFSVDAVSHALLPRQAVLEMA
                     RVTRPGGYILVIDRQQKSRSLGADPWEQPVTAEQIVDWLRPVARQTKVQQFPISAGTL
                     GRTPYFAVLVQRGR"
     gene            1858..2475
                     /locus_tag="PLD05_04430"
     CDS             1858..2475
                     /locus_tag="PLD05_04430"
                     /inference="COORDINATES: protein motif:HMM:NF020279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HOP76711.1"
                     /translation="MSQFEENSRSRDFGETVPPERSQRRGCLWILVFVGVLVIIGCVA
                     LCLGVFAVVLSMGKEPYQMAVRLVTQDAQVVEKLGKPIEVASWFPQGSIHVRGDQGDA
                     NLTFQIRGPQGKATVNVVARRIKGKWGLTSLDVTYPDGTRQPIDVSAEGDSELDAPQW
                     QPGAQVPTESTQPAGEEPSQSEDQGREEGPAPPSLEVKIPELPKL"
     gene            complement(2487..3965)
                     /gene="glgA"
                     /locus_tag="PLD05_04435"
     CDS             complement(2487..3965)
                     /gene="glgA"
                     /locus_tag="PLD05_04435"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOP76712.1"
                     /translation="MRILFASSEAVPFATTGGLGDVCGSLPFALANLGHDVRVIIPAY
                     RGIWYAGREIRSLGLEFIVSVGSKTVSGRLLESRADNVTFYFVQQDYYYDRDALYNVH
                     GQDYADNCERFVFFDRAVMEAIRLLDFVPDILHVHDWQTGLVPAYLKIEYSQSPLYRG
                     IGSVLTIHNLAYQGRFWHWDMLLTGLDWKYFNWRQMEFYGQLSFLKTGIVFADTISTV
                     SPRYAQEIQTPEFGWGLEGVLRSRRDVLYGILNGIDDTVWNPETDPHIACRYNAQTVF
                     QGKAICKKALQEEMGLPVRPDAPLISMIGRITHQKGFDMVADILPYWLEKEDAQWVIL
                     GTGDTALEQRFREFADRWPQKIAVRIDFCHPLAHRIHAGADIFVMPSRFEPCGLAQLQ
                     ALRYGTVPVVREIGGLADTIVDCRSENAAGGRANGFSFSSEDPWELSRTLQRACETYR
                     QPDLWRQLIQNGITQDWSWRHSAPRYIEMYEDTLRRVRGTNQ"
     gene            complement(5139..5504)
                     /locus_tag="PLD05_04440"
     CDS             complement(5139..5504)
                     /locus_tag="PLD05_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76713.1"
                     /translation="MGTRPVGNTVISLSVTGYHQDVPRFGWVHGDRYRFGTWSPDSQR
                     RQVPIAIDLQDGRWGGETVGKLHQYGVLGISHNMPIGDHKAVIRADRDQRSSALENCS
                     GVQGKNAGHGGMGRRWCAC"
     gene            complement(5903..6136)
                     /locus_tag="PLD05_04445"
     CDS             complement(5903..6136)
                     /locus_tag="PLD05_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76714.1"
                     /translation="MNGEELSELAALSLHPWASASEAPYGTLVLVAAMFWGVTPDDSA
                     AYECSPGEPCLPSGDDPAETPLPAPWGPATSGT"
     gene            7497..7832
                     /locus_tag="PLD05_04450"
     CDS             7497..7832
                     /locus_tag="PLD05_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76715.1"
                     /translation="MSSSAGNSGSTTAENGNSADDAEQASKGQERANASSTASEPTEK
                     QPSPTKTTPGWESMIGIAGLLILFIQSMRWLSLLYDRSYYQRRRSYRPVRRRTTWTPT
                     TPPAYRWYY"
     gene            7979..8980
                     /locus_tag="PLD05_04455"
     CDS             7979..8980
                     /locus_tag="PLD05_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOP76716.1"
                     /translation="MTLLYYDPFFLQHETGAHPERAERIAGVLEAFEDQKLLQQCRRP
                     TWKAVSRSRLTLIHSPRYIDEVWAVAKSGGGDLDHDTICSPSSYDVALFAAGAVCDAV
                     ERVVRGEDRRALCLIRPPGHHALRERAMGFCIFNNIAIGAAVAIWELGLERVLIVDWD
                     IHHGNGTQAAFWEDPRVGFLSIHRYPFYPGTGTEDEIGEGLGKGYTKNIPVAYGISRV
                     DYRETLAQALEEFAAFVKPQLVLLSAGFDTHRLDPIGNLGLETEDYEPLTAMVLDIAD
                     TYAEGRCISVLEGGYNRLVTPECLSVHLRTMLERDQGERGRSVSDDLAQKEDTDRNT"
     gene            8993..10486
                     /locus_tag="PLD05_04460"
     CDS             8993..10486
                     /locus_tag="PLD05_04460"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOP76717.1"
                     /translation="MSKPLLECRAIVKRFGGILALDRVDFDLYPGEVHGLVGSNGAGK
                     STLMKILAGAIPDYEGTVLLDGQPVNLRDPHTALKLGIAMVYQEFSGIGQLSVAENLF
                     LGRQPLTKWGIVDWRAMVSWAEEYLHDLGIKLDVSRRLDRYPLVVRQMVEIIKGAYRG
                     ARVLILDEPTSALSPPEIERLFELIRRLKSRGVAMIFISHFLEDTLRISDRVTVLREG
                     RRVLTRLCHEVTKQDLVRAMVGERCEEMAMADQQVVTLPPPSQNPPVLAAEDLTLRGV
                     FEGIRLQVAPGEILGLYGFVGAGHQELVQAVAGAQLPTRGRILLDGQPLPLGKPRAAV
                     RKGVVLVTADRAAGLFMRGEVYKNVTMAYLEQAVGEWITARREVMAVQPVLERVACSP
                     PDPWMKAGQLSGGNQQKVVFAKWLLGPVRVLLLEEPTRGMDIAAKEDVMRLVQDLAKQ
                     GVAVILASTEPELILRYAHRIIVFSRGRITASWAGETVTKQDLMRAA"
     gene            10587..11597
                     /locus_tag="PLD05_04465"
     CDS             10587..11597
                     /locus_tag="PLD05_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76718.1"
                     /translation="MANWRGIFRRWEPQDIAPVVSLAALIVFFSIAAPGFFRWNTLVT
                     AMQQGAVLAIVSAGLTFVLLAAEIDLAVGAVALWTACCCGWLFQNWATQTTEGRFVAS
                     PGFVATVIVLPLLTSLLLGTLSGFLTVQSRLPSFIISLAMMFISQGAARYITRGQAFR
                     VPELLNYVGNRGLEHEGLTVVPYSAMLAAVIMLVGHIVLQYTRFGRYVYMTGGNRQAA
                     RLAGIPTGVIVTACLAISAVTAGIGGLVNAGRMTSVTLDQNQDLLLSALACIVLGGTS
                     LFGGEGSMGKTLVGVATFTVLNIGLNSIRLDDFARPFLMGVILLASLLLNAILSRRQQ
                     WS"
     gene            11848..13113
                     /locus_tag="PLD05_04470"
     CDS             11848..13113
                     /locus_tag="PLD05_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HOP76719.1"
                     /translation="MNREKTPAPFDVTELRQQFPALSQRRDGQVCVYFDGAAGTQVPH
                     RVVDAVCRYLIETNANHGGVFATSRESDAILEAARRLLAEFLGTPDSDTIVFGPNMTT
                     LTFALSRSLMKTWKPGDEIVVTDLEHDANVTPWVLAAREVGVNVKSVRINAADGTLEL
                     EDLHSKLSRKTRLLAVTCASNALGTVTPIAEIVHLAHQVGALVFVDAVHYAPHRLIDV
                     MAWDCDFLVCSGYKFFGPHVGVLWGKREWLVSLPCYKVRPAPDEIPYRWMAGTQNHEG
                     IAGLHAALEYLMEIGHRAHPEPSTPRECFAAAFRAIQEHEQELTKVLLEGLAALPEFK
                     VWGISDLTRLRERVPTVAVTHRRLTPKELAEFLALRGIFVWHGNFYALPVTQALGLEP
                     DGLLRISMVHYNTKEEVYRLLAAFRELRD"
     gene            13221..14000
                     /locus_tag="PLD05_04475"
     CDS             13221..14000
                     /locus_tag="PLD05_04475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP76720.1"
                     /translation="MEVVRQSVYDYPKYYDVLFGSDWKAEYDFLCDCFEYYAKRPVRR
                     VFEPACGTGRLLFRFAQAGYEVAGLDLNEKAVEYCNRRFQRLGLSAHVWVGDMADFRV
                     KRKYDAAFNTINSFRHLTDDKSAMGHLACMREALAKGGLYILGFHLTPNRPPKCTEES
                     WVVRRGHLQVNSWMKTLSLDRQRREEKVYLQFDVYTPRRSFRIVNEFVFRTYTWQQFH
                     RLLQRVGGWEIAGTHDFAYRIDEPITVDDYTEDVVYVLRRV"
     gene            14089..14898
                     /locus_tag="PLD05_04480"
     CDS             14089..14898
                     /locus_tag="PLD05_04480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOP76721.1"
                     /translation="MTSAQHSQSELERFLRIADMAVDAAAEVLLTKFGRVEAQLKGYA
                     DFVTEADMGAQQAVVEIIHREFPHHDFLGEEGSRGGSSQSSYRWILDPLDGTTNYVHG
                     VPFFAVSLALEYEGRPIVGIIYDPMLRERFTAVTGWGAQLNGQPIRVSHVKRLSDALV
                     SIGLPARVVPDSVDVHWMIQSAVHCLSIRRTGSAALNMAYLAAGRFDISWALTTKAWD
                     VAAGAILIEEAGGVVTGLDGQPLNAQGGPYVAAANRDLLDQFLAVVGPFRK"
     gene            15009..16571
                     /locus_tag="PLD05_04485"
     CDS             15009..16571
                     /locus_tag="PLD05_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76722.1"
                     /translation="MSHEHTTPHGKIRPHCCPCCDSSVPINLGEFTRRTFLGGATLGT
                     ALFAGLTWAKLAEETPELPMPPGRLPLKVKPVFLYHVYQPRPQTSWRPWGGIQTEEAA
                     AEEVKRIQGELEKLQAAADFPMEIAPLTATKNAAELAQMPDVQSADVLLVYANDGDLN
                     AIAALKKDTIFFIRHKSGPVYLYYEIISPRFLRQHTDELKVPGMDNDDVVIDNYDEVL
                     WRLRALCGLKNARNQRIIAVGGAGAWAQPPKVVPELVKKIWNTEIIDLPYPELGKMIQ
                     AARSDQAAVNLAKRRAEEYLKDPMVKLETDRTYVENAFLLDQVFRKVMAQANCRMITI
                     HGCMGTIMPLAETTACLTLSTLNDDGYLAFCESDFVVIPSGMLLCGISGLPPFLNDPT
                     YPHDKIITLAHCTAPRKMNGKEKEPARLLTHFESDYGAAPKVEMRNGQVVTNILPDFK
                     SERWVGLRGEIVAHPFLPICRSQIDIKYTCDDKILADRMPGFHWMTIYGDYIREAGYA
                     LKRVGIKFEVLG"
     gene            complement(16657..17859)
                     /locus_tag="PLD05_04490"
     CDS             complement(16657..17859)
                     /locus_tag="PLD05_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOP76723.1"
                     /translation="MKAPFGRCERSFDKSGDRRKGDLVWTVLWGLFLVWEAAVGFGQD
                     VAPVHHVPWLGDVQMGLALGNDQNIPMNLLVRPDGSRIARAEQWRAYREGIRKWWLEF
                     LKLPERDIHRSLATMQVMEKAETDGVVRCLIRYETPGNWPTEAFLLFPKDTNPLESNK
                     TLLPAVIVFHSTTNDTIRQPAGLTAETTKAFGLQLAKRGYVTLCPRCYLWSDGPPYDY
                     QAQVRRYQRSFPGSKGMGKMLADGMAAVDLLTQLPGVDGQRIGAIGHSLGGKEVLYLA
                     AFDERIRATVSSEGGVGLSFSNWDAPWYLGPEIREPQFGHDHHELLALIAPRAFLLIG
                     GDSADGEKSLPYLQSAWQVYRIYTERPALGLWNHRQGHSVPHEAEQRIAEWFDVYLRE
                     KVISPSTE"
     gene            complement(18063..19652)
                     /locus_tag="PLD05_04495"
     CDS             complement(18063..19652)
                     /locus_tag="PLD05_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP76724.1"
                     /translation="MRPGLSKLRYRMRWDRGPEAFRMGIDLLAAALLITICLSGAVFA
                     ADGVDNPNIVVILADDLGYGDVSSYNPDRGKIPTPNIDRLAKEGMRFTDAHSSSGVCS
                     PSRYTLLTGRYHWRTRLQAGIVNVFGEPLIAPDRMTIGTLAKARGYRTACFGKWHLGW
                     DWPIPAEEMKYFRVARNQKETPPPTKEQVEAWQRVFSQRIPGGPTERGFDEYFGTDVP
                     NWPPFCFIKNDRTVGIPSVLLPDRFLGAEPRMASLPGPALPDWRFGDILPTITDRACE
                     FIRQCGEDGKPFLLYFPLTSPHEPLAVNEPWKGKSGLNVYADFVMETDAMVGRVLDTL
                     DEIGVTDKTLVIFTSDNGKARYTGAEALEKMGHFSSGPFRDYKASVYEGGHRVPFIVR
                     WPKVVPPGSVCIQLVHQADLFATLAEILEVPIPPNAGEDSFSLLPLFRGENRSVRPHA
                     VSCASSGVPSVREGPWKLILNKNPELYQLDNDPGETANLADRYSDRVEAMKKLLEKII
                     RDGRSTPGPQQKNDVPVKRYP"
     gene            complement(19960..20280)
                     /locus_tag="PLD05_04500"
     CDS             complement(19960..20280)
                     /locus_tag="PLD05_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76725.1"
                     /translation="MLPFTNGFGNVLDVRQRSCGRVETRRIVWRLKAQRLPHAQRDSP
                     FTRRVWKVIPLGSSPRRATRCPALGNHEASIANALETTARPHTSRMVVVKPAALGYFL
                     LQAN"
     gene            20538..20669
                     /locus_tag="PLD05_04505"
     CDS             20538..20669
                     /locus_tag="PLD05_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76726.1"
                     /translation="MTADKGLPRKSRRIVRRPATRFPHLLKRMRQRLLDRPKKGRAT"
     gene            20735..22582
                     /locus_tag="PLD05_04510"
     CDS             20735..22582
                     /locus_tag="PLD05_04510"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOP76727.1"
                     /translation="MGYRTLRECVKDLLATRQMIRIDVPVDPDLEAAEIQRRVFRAGG
                     PALYFASVRGSRFPMVSNLFGTMERIRYIFRDTLDQLSKLVSLGLDPSDWLRRPRLFL
                     KVPWWGWKAWPKVVSGGPCLKHEIRLQQLPKVRSWPNDGGAYITLPIVYTEDPTQPGF
                     AHSNLGMYRIQITGGKYDPECEVGLHYQIHRGIGIHHARAIEAKQKLRVNVFVGGAPA
                     MTVAAVMPLPEGMSELFFAGLLGGHRIPMIRRKGELPIYAEADFVLTGYIEPKKLLPE
                     GPFGDHLGYYSLVHDFPVMHVEHVYHRPNPIWPFTVVGRPPQEDSMFAQLIHELVGPV
                     IPKAIPGVRAVHAVDAAGVHPLLLAIGSERYTPYDERKRPQELLTLANALLGHGQLSL
                     TKYLFIVAGEDNPDLDVHDVDDFFRHVLERVDWRRDVHFQTCTTVDTLDYTGGALNQG
                     SKVTIAAVGKPRRTLPVALDSRIKIPEDLGFSDPRVMLPGILVIQGPPYRRNEKGEDE
                     SIRQFCARYSRHDAVNLFPLIVIVDDSEFAARTLNNFLWTTFTRSDPAGDIHGIEEFV
                     SKKHWGCHGSLVIDARAKPEHPPPLVEDPEVVRRVEALAAPGKPLHGII"
     gene            complement(22862..23719)
                     /locus_tag="PLD05_04515"
     CDS             complement(22862..23719)
                     /locus_tag="PLD05_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76728.1"
                     /translation="MSRSMWRIAALVGAFLLGLSATWLLGADPVVKNQNPPAPVATAE
                     TIDLFAAIDQKLVEVKLIPKDSTECNVVVKNKTDKPLTIRMPETFAGVPVLAQFGGIG
                     GEMGYGGRRGRGGYGGGGYGGGAQGFGGGFGGGYGGYGGGYGGYGGGMWNVPAEKVVQ
                     AKIPIVCLDHGKPDPNPRIPYEIKPIESYAKCPEVNEVLKMLASGTIPQRVAQAAAWH
                     FQNGLSLEELARKEIRTAIGLRRPYFSPEEMRAAIQVVAQANRVVMNSGERSVGTPSP
                     GEVEAARQN"
     gene            24340..25644
                     /locus_tag="PLD05_04520"
     CDS             24340..25644
                     /locus_tag="PLD05_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76729.1"
                     /translation="MSQSTRRTFLKVSAGISASTLTARQYARAWEANRRLVIGIIGCG
                     GRGGGLAQAFAEISDIAYVCDPDRSRCERLQGAVKAKKAVTDPRQVLDDRSVDAVVIA
                     TPDHWHAPLAILVCEVGKHVYVEKPLSHNFRESQLLVEAARRARVVVQHGTQARSSPL
                     INKAIQLLHTGVIGDVLVAKAWNVQRRVNIGHEQPSEPPPGVDYDMWVGPAEFVPFQK
                     NRFHYTWHWWYNFGTGDIGNDGVHELDYGRWGLGVSGLPNHVMAEGGKYFFDDDQEFP
                     DTVTCAFEWTGDGKIGNKRQLILEMRIWSTNWPYGEENGVEFYGTEGRMVLAKDRKIE
                     IYGERDRFRDDIKLDDLPQMPPSHQADFVEAIRTGKKPTADVIEGHHSVALTHLANIA
                     VRVGRALRVDPQRETIIGDEEASRLLRRNYREGGHWAIPKGL"
     gene            complement(25787..27601)
                     /locus_tag="PLD05_04525"
     CDS             complement(25787..27601)
                     /locus_tag="PLD05_04525"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOP76730.1"
                     /translation="MLRTSTTTFFVLATFVLFPMQSSPAQELVIAENGKSEYCIVIPE
                     QASEPVRHAADELARFIGEMTGANLPIRTENFQPAAECEILLGNNKRLQELGLEIDWA
                     KLGKEGYTLKTVNKKLVIAGGEPRGTLYGVYGLLEDHWGCRWFTPDCSRIPRYDRLVI
                     GPLDETVVPVLEYREPFTYDCFDGDWCARNRMNSSSARLGPQHGGKVRFGAGFFVHTF
                     DRLVPPEKYFDEHPEYFALVKGKRLKTRTQLCCTNEDVIRLVTEGILKAIENDSEAFV
                     FSVSQNDWYNYCECENCQALAKAEESQIAPVLQMVNRVAEAVEKRYPDKAIETLAYQW
                     TRKPPKTMRPRPNVIIRLCSIECCFSHPLATCDLPANRAFVEDLQGWAKIGNRLWVWD
                     YVTDFRHYYLPFPNQRVRNDNIKLFVANNVKGIFEQDTYNTPNSELAALGGYMTAKFL
                     WNPDYDENLAMNEFLEGYYGAAAPLIRKYIDLLHDRVEKENIHCSIWIPPTHNHLTDE
                     LLVEADQLWQAAEEAVKDDPTLLKRVRIGRMSVDYAILERARTAPDKEPLGSLAKVRF
                     QPFIDMIQPAGVTRLNEWNPLNLDEYRTKLASALRIKP"
     gene            complement(27734..28060)
                     /locus_tag="PLD05_04530"
     CDS             complement(27734..28060)
                     /locus_tag="PLD05_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76731.1"
                     /translation="MLIAEGYDTRDPEGYKTPPPMIALSFKSPLIDLCSSGRHYGVKV
                     DDISLLRLITWVDAMCPYQGEEEIREIPDPEFQGIDWLAIRPRIKTAPKVVRPGPVDE
                     THLPDR"
     gene            complement(28315..29343)
                     /locus_tag="PLD05_04535"
     CDS             complement(28315..29343)
                     /locus_tag="PLD05_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOP76732.1"
                     /translation="MGILTGWLDLVFPPVCVVCGKPVAIETGLAEWRPLLEERVRRHP
                     DRAYDLLWLGGKPPQPGVSNFLCPHCLAILSFSGWISCPKCGGQVDDESPSPDGCDWC
                     RRERFAFKGVVSLGPYAGPLGKVILRMKHPSGERLAHCMARLFCCERGEKLRELAIDC
                     VVPIPLFARQRRRRRINSPEILAETIAKYLGVSLETTLLRRVRDTRPQRTLKPRERWK
                     NVRDAFSVEVSIHDVWRSKLATFWHHAIIRQGPTADTSHDQDEHAKLTSAVRANRGPY
                     HPRLRDRRVLLVDDVLTTGATCHAAAEALLRAGAREVFVAVLARGQGDDDSVAAAMPL
                     HETEFRPW"
BASE COUNT         6509 a         8427 c         8231 g         6519 t
ORIGIN      
        1 ttgacagagg tggggcttcc tcgccccgaa gccatttctc aatagcacaa tttgctgtac
       61 ttaaatcacc ggcccctctg aaccagcacg gcaaaataag gagttcggcc aagggttccc
      121 gctgagatgg ggaactgttg cacctttgtt tgtcgggcaa cgggccgtaa ccagtccacg
      181 atttgctcgg cagtcacagg ttgctcccag gggtctgctc ccaaactgcg agacttctgc
      241 tgacggtcga tgaccaagat atatccacct ggccgggtca cgcgggccat ttcaagcacg
      301 gcctgccgcg gcaagagggc atgggaaacc gcatccaccg aaaagacggc gtgaaaactc
      361 tgatcggaaa cgggaattcg cagtaggttg ccttcgatgg ttttgattcc gcgtggtacc
      421 tgccggagtg cctcaggcac gaaatcaatt ccgaaccact gatgatgggg aaaccaattt
      481 gacaggaact ttaggtaacg tccggttcca cacccaagat cggccaagga gctcccttgg
      541 ggcatggtcg caaccagaga acggaccgca agcaggcgag gatcttccgc atcgagaggt
      601 tcttcgtttt taattcgtcc tcgataagca catcggaccc gccaaagcgt cgcctcaaga
      661 aaatactttg ccgccaagcg gtcaggctca cgatcatttt ccgaagtgcc ccgggcactt
      721 cgcggaaaac ttccatcagg tagtcgcctc ttctccacga atcggtacgc ctgctcaccc
      781 cgcgtttcat tgaacatgcg aaaccaaagg agcgccgcct gggcaacggt ggccaagagg
      841 atgaccgacc cgttgtcttg ttcaggtacg gcccccgcgg cggtttgccg ccgatcgagt
      901 ttttcaacca acggaatagc atcctggcgg tggccccaat cgagaaagaa gatggcccgg
      961 cgaacgatgg ccgagctctg ctcagccggg acacggcggt gcccgtgatg acgccagtaa
     1021 tcgatggccc ttagaaccac ggtcgtccaa tccgtatcgg gccaacggcg tcctgcccat
     1081 aaaaggggcg acaaatcggg cggctcatta aacagtgtcg tcggttcgta gttccaggcg
     1141 cgattacgcg gcggcaagag gcgcccttcc tctcccaccc attgcctaag gaaaacacac
     1201 gccttctggg ctgccccctc gaattggtta agttccggca gggccgccag ccaaccgcgt
     1261 gcggcagccg ctgtggcgga aaaaaccggc tccgcagccc gggactccga aagcgagcca
     1321 tcaggaagtt gcctctcttg aagccaacga ccgatccgcc aggcagcctc gcggtagccg
     1381 aagtgcatca tcacctccat gaccaggccc gcggccacga tgacggacgg cgagggcgcg
     1441 gccttttcct cccatttttc cgacacccgc atgagccact ccagagtgga ttcgatcccc
     1501 tctggagtgc gactgaaaag gcgagaaaga aagccccata atccccgggt tcggcccgtt
     1561 gtaccgcgat cgtccggttc ggtggcctgg gattcggaat taccaggtgg tacgggagca
     1621 tccgatcgaa ccatggcggc atcctcgagt ttcctcgcca attggcgggg actggggacc
     1681 atcctatgaa gccctcagtc gggccatttg tgtcgcattc gtgaagcctt ttgccaaccg
     1741 gttttatctc ccgagacaaa atggcctttc ttttcggggc cggtgaacct tatgataagc
     1801 actggtttgc cgaatctcaa gaaaagttgt gccacctcac ataaggagcc ttttccaatg
     1861 agtcagtttg aggagaattc gcggtccagg gattttgggg aaacagttcc gcccgagaga
     1921 tcgcagcgcc gcggttgcct ttggattctc gtctttgtcg gcgtccttgt gatcatcggt
     1981 tgcgttgccc tttgtttggg cgtgttcgcc gtggtccttt caatgggcaa agaaccttat
     2041 caaatggcgg tgcgcctggt cacccaagat gcgcaggtcg tggaaaaact ggggaagccg
     2101 attgaggttg ccagctggtt tccgcaaggg agcatccacg tccgagggga tcaaggagac
     2161 gccaatttga cgttccagat tcggggacca caaggaaagg ctaccgtcaa tgtcgtcgcg
     2221 cggagaatca aaggaaaatg gggcttgacc tcgctcgacg tcacgtaccc cgatggcacc
     2281 cgacagccta ttgacgttag tgcggaaggc gattcggagt tggatgcccc tcagtggcag
     2341 cccggcgccc aggttcccac ggagtcaacc cagcccgcgg gcgaggaacc atcccagtcc
     2401 gaagatcaag gtagggaaga aggtccggct ccgccgtcac tggaggtaaa aatccccgaa
     2461 cttcccaagc tgtgagggga cagcctctac tgattagtgc cgcggactcg gcggagggtg
     2521 tcttcgtaca tctcaatgta ccgcggagcg ctgtgccgcc acgaccaatc ctgagtaatc
     2581 ccgttctgga taagttgtcg ccaaaggtcc ggctgtcggt aggtttcaca agctcgctgc
     2641 aatgttcgcg acagctccca gggatcttcc gaggaaaagc taaacccatt ggcccggcct
     2701 cccgccgcgt tctccgagcg gcaatcgacg atggtgtcgg ccagtccgcc aatctcgcga
     2761 acgaccggaa ccgtaccata acggagcgct tggagttggg cgaggccgca cggttcaaat
     2821 cggctgggca taacgaagat atctgcaccg gcatgaatgc gatgggccag ggggtgacaa
     2881 aagtcgatcc gtactgcaat tttctgcggc cagcgatcgg cgaactcacg aaatcgctgt
     2941 tccaaagcgg tgtccccagt tcccaaaatc acccattggg catcttcttt ttcgagccag
     3001 tagggcaaga tgtcggccac catgtcgaaa cctttttggt gcgtgatccg cccaatcatg
     3061 ctgatgaggg gtgcgtccgg ccgaacgggt agccccatct cttcctgtag ggccttcttg
     3121 caaattgcct ttccttgaaa aaccgtctgc gcattataac gacaggcgat gtgggggtct
     3181 gtctccggat tccacacggt gtcatcgatg ccgttgagaa taccgtagag cacgtctcgt
     3241 cgagaacgaa ggacgccttc cagtccccac ccgaactcag gggtttgaat ctcttgggca
     3301 tatcgtgggc tgaccgtgct aatggtatcc gcaaacacga tacccgtttt caaaaagctt
     3361 agttgcccat agaactccat ttgtcgccaa ttgaagtatt tccaatcaag gccggtcaaa
     3421 agcatgtccc agtgccagaa gcgaccttga taagccagat tgtgaatcgt caggacgctt
     3481 ccaatccctc gatagagcgg cgactggcta tattcgattt tcaagtaggc cggtacaaga
     3541 cccgtttgcc aatcgtgaac atggagaatg tcgggcacga aatcgagcaa ccggatggcc
     3601 tccatgaccg cacgatcgaa gaagacgaac ctttcgcaat tatcggcgta atcctggcca
     3661 tgaacgttgt agagagcatc ccgatcgtag taataatctt gctggacaaa gtagaaggtc
     3721 acattatccg cgcggctttc gagcagtctg cccgagacgg tcttgcttcc cactgaaaca
     3781 atgaactcaa ggcctaggga acggatttcc cgacccgcat accaaatgcc gcggtaagcg
     3841 ggtatgatca cgcggacatc gtgtcccagg ttggcgagtg caaagggaag gctgccgcag
     3901 acatccccta atcccccggt ggttgcgaaa ggcacggctt cactgcttgc aaatagaatc
     3961 ctcactggcg tcacctccgg acggcttacg gtcttagaga gcatgccgaa tcactcggca
     4021 aaacattttg cccagtcgcc acaacaaggc agaaaccctt aacagtattc tggcgctgag
     4081 ggatacccca ttaagtctag ctcaggattt cggggaagga agtttttgtc gatacaaatg
     4141 ggcacttcgc tttcaactca taaaaccatc aaatatacca gattcatctg aattttacgg
     4201 ctgcctgtgg tgacgttgga tgacgaacaa ggctcctttc caaagggaaa acggaccggc
     4261 gtggggccaa tatcgcagcg atacggctta gagcctatcc cataatctcc cgtcgcccgt
     4321 tcggcaggag aggggttggg ggtgagggtg gctggaatcg ctgccggttt tctcaatcga
     4381 ttcccacatt cgtttgccct caccccaacc ctctcccaga gggagagggg gactttttgg
     4441 cataggctct cggtcgaggc ttatcttctc aaccggtatt gatttgcgga aatgtcgtca
     4501 ccaagagagt gcttcatgtc gttcgggtca tatcgaagac aagtagccta tgcgatgtat
     4561 tagctgacgg ggagaaacgg gtgatttcag tcgttttcct tggacgtgtt gtgagtcaga
     4621 ttttggtctt gcggaccacg ggaggcatgc tcattttgtg ttcgggaatg ttgacaaaaa
     4681 gaaacttcgg gcaacgccct ccttatgccg aatgctagat gaaggggtct tgccccggtc
     4741 agaaaagtct cgccacagga tggttctttt cctccgctca tggctgtgga ggagcacgga
     4801 tgccgcaggg atggcacctt gattgatttg acaataaagc ggcattacgg ctcgctgatt
     4861 caccaaacgg tgaagtgatc aattcgcaga tctgttaaag cggcagcagt ttgcggttgt
     4921 cgtaacggac atttgtcccc attggtttga cccgtttggg gatgcgtgtc tagggtttaa
     4981 gccgttcagg atgtccatga atgttttttg tccgatgata gcttcaactc tcgcgacgtt
     5041 tgcgtaatgt gaaaacgccg gtccttcaaa tcctttgtcg aatcttcctg tggctcgtcc
     5101 tttgtgggag cgcgggggag gtttgtgcct ggctttggtc aacaggcaca ccagcgacgc
     5161 cccatcccgc cgtggcccgc atttttgcct tggacacccg agcagttttc aagggcactg
     5221 gaacgctggt cgcggtcagc tcttatcacg gccttgtgat caccaattgg catgttgtgc
     5281 gagatgccaa ggacgccgta ttggtgcaat ttcccgacgg tttcgcctcc ccagcgacca
     5341 tcttgaaggt cgatcgcgat tgggacttgg cggcgctggc tatctggcga ccacgtgcca
     5401 aaccggtacc gatcgccgtg gacccaccca aacctgggga catcctgatg atagccggtt
     5461 acggacaagg agattaccgt atttcctacg ggccgtgtac ccaatatttg tctcccgccc
     5521 caaatcttcc cgctgagttc gtagaggtgc ggaccaccgc gcgtcacggc gattctggcg
     5581 ggcccatctt taatgcccgg ggtgaattgg caggagtcct ctttggctcc ggatccgagg
     5641 acatgatcct ctttcggctg ggatccggat ataccgttgg ggcctactgc ggtcgggtac
     5701 gaagcttctt attaccgttg cgcgacgtgt tcaataatct cccgccgccc gacatcctcc
     5761 tagctcaggc aatgagtgcg ggagctcccc cggttactac gaacggcagc acccaggtac
     5821 cttcacaggc agggagtgcc ccaactgccg gatcttacgg gattgctgct acgacctctg
     5881 gggctgcggg aagttcccct tttcaggtgc cgcttgtcgc ggggccccag ggagcgggaa
     5941 gtggcgtctc cgccgggtcg tcccctgatg gaagacaagg ctcgcccgga ctgcattcgt
     6001 acgccgcact gtcatcaggg gttacgcccc agaacatcgc tgccaccaag accaacgtgc
     6061 catatggggc ttcggacgcg ctggcccagg gatggaggga aagcgccgcc aattccgaga
     6121 gttcttcgcc gttcacggct cgctcgaatg agctcgccgg tgggcagcaa gggtcgtccg
     6181 acggccaaag tcacagtggc gagccctatc ggagttccta cccaggtcaa aacaacactt
     6241 atagctccgg ttcgtcggca gggacagaat cctcctatgc ctattctggg cgggagagca
     6301 cgcgatcaga ctggtcgtac ggaccatcct ctccctacgg cagggatact agcgaaataa
     6361 ctaattccat ggcaaacagc agaaatgagg gcccctctta tggcggcggt tcgtcttggg
     6421 actccacttc caaagcctcg agtgccgcaa gtgaatatgg gtacgcttcc tccacttctg
     6481 actacggata caactcctct ggttccgatt acggatataa ctcctccggc tctggccacg
     6541 cgtcatactc gtacgatagc cagaaaggtc agcaaccttc gggcgaatcg tatcgtcaag
     6601 agagtaactc gttcgcctct gatgaaagaa ggagtaccgc tcaagggggt tatgccacgg
     6661 ctggagaaaa caataagcgt aacggcgaac gttcacgtga caacaatttt gcccgaaatt
     6721 cttcgtcggg ctatgacagc tcacaatcgc cttatgggtc ggaagaagat aacgtcccgc
     6781 cgtattctta cggcttggaa cgtaccggaa tatccgagta tgcggctggg aacgattcgt
     6841 ggggttcaga agatggtacg ccggccggcg gtctatcgtc cacgaactcg ttgcggcgag
     6901 atcggcaaaa ttcgcgaagc tacacggcga gtatcaatac cggatcaaat tcaacgaagt
     6961 catcggagag ggacgactcc gacgaggatc ggtattcatc acccgatcaa tcagatcttt
     7021 ccaaaactat ctccccgaaa caatctaaca actcagcctc caccggctcg tattccggat
     7081 actcggccat ccctgtctat gacgaagacg acaacggttc ccgcaatcgc ggaagtgcca
     7141 caacagcctc caaaggctca tcttcttggg acacgaactc ggatcacagc ggtacttcct
     7201 ccgaggacgg tgaccgcggt gggtcggcgt ctcgttcaag cggttccaat tcccggtcct
     7261 cgtgggatca atccgggacg tcgtatcgtg agtccaattc tggaacttcc ggctcaggaa
     7321 actcgtcccc gaaaggctat caaggaggta aaaaacctcg cagctccagt tcatcccatt
     7381 gggatggatc cgaggaacat tcgagcaatt cctggagcag tagcagttca ggacgcgcaa
     7441 cgtcccggaa ctcttcggag acgggtacca gtgattccct gtctcacgga gcatcattgt
     7501 cgagttcagc cggcaattct ggcagcacaa ccgccgagaa tggcaattcc gcggatgatg
     7561 ctgagcaagc gtcgaaaggc caagagcgtg caaatgctag cagcaccgca agcgagccaa
     7621 ccgagaagca accttcgcct acaaaaacaa caccgggctg ggaaagtatg atcggcattg
     7681 ccggtttgct catcctcttc attcagagca tgcgttggct cagcctcttg tacgatcgaa
     7741 gttactacca gcggcggcgg agctatcgac ccgtccggcg gcggacgacg tggacgccga
     7801 ccactccgcc cgcatatcgg tggtactatt gaacgaccat gctcacttct cctgattttc
     7861 tctggccgag aaactagaat aaccgttaag aggtatttcc gcaaacccat acaaccgagc
     7921 acgacgcgga aaataacgat ctttttcctg tgcacgtgcc agtcggctgt ggtgccttat
     7981 gacactcctt tactatgatc ctttcttctt acagcacgag accggagccc acccggagcg
     8041 ggccgagcgg atcgctgggg tgttggaagc ctttgaagat cagaaacttc tccagcaatg
     8101 ccggcgtccc acatggaaag cggtctcccg aagtcggctc acattgatcc attcgccccg
     8161 atatatcgac gaagtctggg ctgtggcaaa atcgggtgga ggtgatttgg atcatgacac
     8221 gatatgcagt ccctcgtcgt atgatgtagc acttttcgcg gcgggagctg tttgtgatgc
     8281 cgtcgagcgc gttgtccgtg gcgaagatcg ccgtgcgctt tgtttgattc gtcctccggg
     8341 tcaccacgcc ctgcgcgagc gtgcgatggg gttttgcatc ttcaacaata tcgctattgg
     8401 cgcggcggtc gccatctggg aattggggct ggaacgtgtc ctgatcgtcg attgggacat
     8461 tcaccatgga aacggcactc aggcagcatt ttgggaggac ccacgtgtgg gattcctctc
     8521 gattcatcgc tatccgtttt atccgggcac cggaaccgag gacgaaattg gcgaaggact
     8581 aggtaaaggt tatacaaaga acattcccgt agcatatggg atctcccgcg tcgattaccg
     8641 ggaaacgtta gcacaggcct tggaagagtt tgccgccttt gtgaaaccgc aacttgtcct
     8701 tctcagtgcg ggcttcgata cccaccggct tgatcccatt gggaatctcg gcctcgaaac
     8761 cgaagattac gaaccgctca cggcaatggt gctggatatc gccgatactt atgcggaagg
     8821 tcgatgtatc agcgtcctcg aaggagggta caaccgcctg gtcacgccag agtgtctttc
     8881 cgtgcacctt cgtacgatgc tcgaacggga tcaaggggaa cgggggcgtt ctgtctccga
     8941 tgatctcgcc cagaaggagg acacagacag aaacacgtga ggagaccaga gcgtgtcgaa
     9001 acctctcttg gagtgtcgtg ccatcgtaaa gcgatttggg ggaattctcg ccctcgatcg
     9061 agtggacttt gatctgtatc ccggcgaagt tcacggattg gtgggcagca acggtgccgg
     9121 caagagcacg ctcatgaaga ttctcgccgg tgcgattccc gattatgaag gaacagtact
     9181 tctcgacggg caaccggtca atttgcgcga tcctcacacc gcgttaaaac tggggatcgc
     9241 catggtgtat caggagtttt cggggatcgg gcaactgtcg gtggccgaga acctgtttct
     9301 cggtcggcag ccgctcacga aatgggggat tgtggattgg cgggccatgg tttcgtgggc
     9361 tgaagaatat ctccacgatc tcggaatcaa gctcgatgtc tcccgccgac tggatcgcta
     9421 tccccttgtc gtccggcaaa tggtcgaaat catcaaaggg gcgtatcggg gcgccagagt
     9481 gctgatcctt gacgaaccga caagtgccct cagccctccg gaaattgaac gactgttcga
     9541 gttgatccgg cggctcaagt cacgtggagt ggcgatgatc ttcattagtc atttcttgga
     9601 ggataccctt cggatctccg atcgggtgac ggtcttgcgc gaaggccgcc gtgttctcac
     9661 acgtctctgc cacgaggtaa ccaagcaaga tcttgtacgg gccatggtgg gggaacgctg
     9721 cgaggagatg gccatggccg accaacaagt ggtgacccta cctcccccat cccagaatcc
     9781 accggtgttg gccgctgaag acctgacact ccgtggtgtc ttcgaaggaa ttcgtctcca
     9841 agtggcgccg ggtgaaatcc tgggactgta cggctttgtg ggggcagggc atcaggaatt
     9901 ggtccaggcc gtggccgggg cgcaactgcc cacccgtggg cgcattctgc ttgatggaca
     9961 gccgttgccc ctgggtaaac ctcgcgcggc tgtgcgaaag ggggtcgtct tggttacggc
    10021 cgaccgtgcc gccggactgt tcatgcgtgg tgaggtgtac aagaacgtca cgatggccta
    10081 tctcgaacaa gccgtcgggg agtggatcac cgcccggcgt gaagtcatgg ccgttcagcc
    10141 cgtcttggag agggtggcct gctctccgcc agacccttgg atgaaggccg gccaactctc
    10201 cggcggaaac cagcagaagg tggtcttcgc caaatggctg ttgggaccag tccgcgttct
    10261 tcttttggaa gaaccaaccc ggggcatgga tatcgccgcc aaggaagatg tcatgcgatt
    10321 agtccaggat ttggcaaaac agggcgtggc cgtgattttg gcatcaacgg agccggagtt
    10381 gattctccgc tatgcccacc gaattatcgt ctttagccgc gggcggatca ccgccagttg
    10441 ggcaggagag acggtcacca aacaggacct gatgcgggca gcatagtcgg ctggatcgtc
    10501 cttcccgcgg aaacctccac gcgtatcgaa caacttataa tcggatctta taccaattcg
    10561 agaagatttc agcagggtga aatgcgatgg ccaattggcg aggaatcttt cgacgttggg
    10621 aaccacagga tattgccccc gtcgtttcgc tagcagcgct cattgttttc ttttctatag
    10681 ctgcccccgg cttttttcgc tggaatacgc ttgtgacggc catgcagcag ggggcggtct
    10741 tggcgatcgt cagtgccggc cttacctttg tcttgcttgc tgccgagatt gatctagctg
    10801 tgggcgccgt ggctctttgg acggcctgct gttgtgggtg gctctttcaa aattgggcaa
    10861 cacaaaccac tgagggacgt tttgtcgctt ctcccggctt tgtcgccacg gtgattgtct
    10921 tgccgctctt aaccagcttg cttttgggaa cgctgtcagg gtttctcacc gtgcaatccc
    10981 ggctgccaag tttcatcatc tccctggcca tgatgttcat ttcccaaggg gccgcaaggt
    11041 atatcacgcg gggccaggcc ttccgcgtcc ccgagctgct taactacgtg ggaaaccgtg
    11101 gtctggaaca cgaaggtctg actgttgtcc cctacagtgc catgttggcg gcagtgatca
    11161 tgttggtcgg ccatatcgtg ttacagtaca cgcgatttgg ccggtatgtt tacatgactg
    11221 gtggaaatcg gcaagccgct cgattggcag ggattccgac aggcgttatt gtgacagctt
    11281 gcctggccat ctctgcggta acggccggga tcgggggctt ggtgaacgcc ggccgtatga
    11341 ccagcgttac actcgatcag aaccaagacc tcctcctcag tgccctggcc tgcatcgtcc
    11401 tcggcgggac aagtcttttc ggcggagagg gtagcatggg caagacgctc gtgggtgtgg
    11461 cgacttttac agtcctcaat attggcctga actcgattcg cctggacgat ttcgcccgtc
    11521 ccttcctgat gggtgtcatc cttttggcgt cgctgctgct gaatgcgatc ctctctcgca
    11581 gacagcagtg gtcgtgatga aaaggcctcg tattgttcac ggaccaggca tcagtgacaa
    11641 tgcgaatgga taagagccta tcccgtaatc tgtcctcgcc cgttcggcgg gagaggggtc
    11701 gggggtgagg gtagctcgaa tcgctgccgg ttttctcgat ggattccgat attcgtttgc
    11761 cctcacccca accctctccc ggagggagag ggggactttt tggaataggc tgtaagtcgg
    11821 taacaggcct ttgatgaacg gattaccatg aatcgcgaga agacccctgc acctttcgat
    11881 gtcacagaat tgcggcaaca gtttccggcc ttgtctcaac ggcgggacgg tcaggtgtgc
    11941 gtttattttg acggggcggc agggacgcag gttccgcatc gggttgtgga tgcggtgtgt
    12001 cggtacctca tcgagaccaa cgccaatcac ggcggcgtct ttgccacaag ccgggagagc
    12061 gacgccatcc tggaagcagc acgccgattg ctggccgagt ttctcggcac gccggattcg
    12121 gacacgatcg tttttgggcc caacatgaca accttaacct ttgccctctc ccggtcgctc
    12181 atgaagacct ggaaacccgg ggacgaaatc gtggtcaccg atctggaaca cgatgccaac
    12241 gtgacgccct gggtcctcgc cgcccgagag gttggagtca acgttaagtc ggtgcggatc
    12301 aatgccgcgg atggcactct tgagttggaa gacctccact caaagctgag tcgcaagaca
    12361 cggcttttgg ccgtcacatg tgcttctaat gccctgggga cggtaacacc gattgccgaa
    12421 atcgtccatc tcgcccacca ggttggtgcc cttgtctttg tcgatgccgt ccactacgca
    12481 ccgcaccggt tgattgatgt tatggcgtgg gactgcgatt ttctggtctg ttcgggttat
    12541 aaattctttg ggcctcatgt gggcgtcctt tggggaaagc gcgaatggct cgtatcgctg
    12601 ccctgctaca aggtgcggcc agcaccggat gagatcccct atcgctggat ggccggcacg
    12661 caaaaccacg aaggaattgc cggcttgcac gccgcacttg agtacctcat ggagatcggt
    12721 caccgggccc acccggaacc gagtacaccg cgggagtgtt ttgccgctgc gtttcgggcc
    12781 atccaagagc acgaacaaga gctgaccaag gtcctgttgg agggcctggc cgcgctgcct
    12841 gagttcaaag tttggggcat aagtgatctt acgcgactcc gcgaacgcgt gcccacggtg
    12901 gccgtcaccc atcggcgact cacgcccaaa gaattggcgg agttcctggc cttgcgcggc
    12961 atctttgtgt ggcatgggaa tttttacgca cttccggtca cgcaagcact gggtttggaa
    13021 cccgatggcc tgctcaggat ttcgatggtc cattacaata ccaaggaaga ggtctatcgg
    13081 ctcttggctg ccttccgcga gttgcgcgat tgaaccaaac cgacaaggcg cggggactca
    13141 tagcgaggct agaacggggt ttgcgtcgtc ccggccgtcc aagtttggct gttcctcgct
    13201 tcggagcttt tggaagcact atggaagttg tccggcaaag tgtttatgac tatcccaaat
    13261 attacgacgt tctcttcggt tcagactgga aagccgagta cgacttcttg tgcgactgct
    13321 ttgaatacta tgccaagcgt ccggtcagac gggtattcga gcctgcctgc ggaaccggaa
    13381 gattactctt ccgttttgcc caagcgggat acgaagtcgc cggacttgat ctcaacgaga
    13441 aggccgtcga atactgtaac cgcagattcc agcgtttggg cctctcggct catgtctggg
    13501 tcggcgatat ggcagatttt cgcgttaagc gcaagtatga tgctgccttc aacaccatca
    13561 acagctttcg tcacctaact gacgacaaaa gcgctatggg acatcttgcc tgtatgcggg
    13621 aagcgttggc caaaggcggt ctttacatct tgggctttca cctgaccccc aatcggccac
    13681 caaagtgtac cgaggaatcc tgggtcgttc gacgcggcca ccttcaggtc aactcctgga
    13741 tgaaaacact gagcctcgac cggcagcggc gggaagaaaa agtttacctt cagtttgacg
    13801 tttatacgcc ccgtagatcc tttcgcatcg tcaacgagtt cgtcttccgc acatacacgt
    13861 ggcaacaatt ccacagactt ctgcagaggg ttgggggctg ggaaattgcg ggcacccacg
    13921 attttgcata ccggattgat gaaccgatca ctgtcgacga ttacaccgaa gatgtggtgt
    13981 acgtcttgcg ccgggtgtag gaactagcac gacctccgtc gcaagattga tacacacaaa
    14041 gcacaccagc caaaaaggat ttgaaacctg tttcactgag aggaaagcat gacatcagcg
    14101 cagcacagtc aatcggaact agaacgtttc ttacgaattg ccgacatggc cgtcgatgcg
    14161 gcagccgagg tgctcctcac caaattcggt cgggtggaag ctcaattgaa aggctatgcc
    14221 gactttgtga ctgaggccga tatgggtgcc caacaggccg ttgtcgagat cattcaccgc
    14281 gagtttcccc atcacgactt tctcggtgaa gaagggagcc gggggggatc gtctcagtcg
    14341 tcataccgct ggattctaga ccctctcgac ggtacgacga attacgtcca cggtgtgccc
    14401 tttttcgccg tctcgctggc tctcgagtac gaagggcggc ccatcgtggg cattatctac
    14461 gatcctatgc tccgggagcg gtttacagcg gttaccggct ggggggcaca actgaatggt
    14521 caaccgattc gggtcagtca cgtcaaacga ttgagcgatg ccctcgtctc catcggactt
    14581 cctgcccgcg tggttcctga ttcggtggat gttcactgga tgatccaatc cgccgtgcat
    14641 tgcctatcca tccgacggac ggggtcggca gccctgaata tggcctatct ggcggcgggg
    14701 cggttcgata tctcatgggc gctgacgacc aaggcttggg atgttgcggc gggagccatt
    14761 ctcatcgaag aggcaggggg cgtggtcacg ggtcttgatg gccagccctt gaatgcccag
    14821 ggtgggccgt acgtggctgc cgccaatcgc gatctactgg atcagttcct cgccgttgtc
    14881 gggccgtttc gcaaatagtc acgctttgag ttgcaaccgc ccctgaggaa cgttatccta
    14941 aaagcgccgc ttgggattag ttcggccatg tccgtttgat atttggtcag tcaccgacga
    15001 gggagtccat gtctcacgag cacaccacgc cgcacggaaa gatccgtccg cactgttgcc
    15061 catgctgtga ttcgtcagtt ccgatcaacc tcggcgaatt cacaaggcgg acctttttag
    15121 gaggggccac tttgggcacg gccctgtttg ccggtctgac ttgggcaaag ctcgcggagg
    15181 aaacgcctga gctgcccatg ccccctggca ggcttcctct gaaggttaag cccgttttcc
    15241 tgtatcacgt gtaccagccg cgtccccaaa cgagttggcg tccttggggt ggaattcaga
    15301 ccgaagaagc tgccgcagag gaggtcaaac ggattcaggg cgaactcgag aaactccagg
    15361 cggctgcgga cttccccatg gaaattgcac cgctcaccgc gactaaaaac gccgcggaat
    15421 tggctcaaat gccggatgtc caatctgcgg acgtcctttt ggtgtatgcc aacgacggcg
    15481 atttgaacgc catagccgct ctcaagaaag acaccatctt ctttatccga cacaagtccg
    15541 gcccagtgta tctttattac gagatcatca gtcctcgttt cctacgtcag cacaccgacg
    15601 agctgaaagt cccgggcatg gataacgacg atgtcgtgat cgacaattac gatgaagttc
    15661 tctggcgcct gcgcgcgctt tgtgggctga aaaatgctcg taatcaacgg atcatcgcag
    15721 taggtggagc cggggcgtgg gcccaacccc cgaaggtggt tcccgaactc gtcaagaaga
    15781 tttggaacac agaaatcatt gacctgccct atccagaatt gggcaagatg atccaggcag
    15841 cccgctccga tcaggcggcc gtgaacctgg ccaaacggcg agccgaagag tatctcaagg
    15901 acccaatggt caaattggag acagatcgca cttatgtgga aaacgcgttt cttttggatc
    15961 aagttttccg caaggtgatg gcccaagcca actgccggat gattaccatc cacggatgca
    16021 tgggaacaat tatgccgctg gccgaaacca cggcctgtct cacgctgagc accctcaatg
    16081 atgacggtta cctggcgttt tgcgaatcgg acttcgtggt gattccttca gggatgctcc
    16141 tctgtggaat ttctggcttg ccgccgttcc ttaatgaccc gacctatcct cacgacaaga
    16201 tcatcacgct ggcccattgc acggccccac ggaagatgaa tggcaaggag aaggagcccg
    16261 cgcgacttct cacgcatttc gagtcggatt acggtgctgc gcctaaagtc gaaatgcgga
    16321 acggccaggt tgttacaaat atcctgccag acttcaaatc ggaacgctgg gtgggcctgc
    16381 ggggcgagat cgttgcccat cctttcctgc ccatttgccg ttcgcaaatc gatatcaagt
    16441 acacctgcga cgacaagatc ctggccgaca ggatgcccgg cttccactgg atgacaatct
    16501 acggcgatta catccgtgaa gcgggctacg ccctgaaacg ggtcggcatc aagttcgaag
    16561 ttctcggctg atgcaatcga gtctttcgac gcttctacgg gccgcaccgt cctctgggcg
    16621 gtgcggtctt ttctttaaga gcagcttaag gtactgctat tccgtggatg gggaaatgac
    16681 cttttcccgc agatagacat cgaaccattc tgcgatgcgt tgctcggcct catggggaac
    16741 gctgtggcct tgccggtgat tccataggcc caaagccggc cgctctgtgt agattcgata
    16801 gacttgccac gccgattgca gatacggcag acttttttca ccatctgccg agtcgccacc
    16861 aatcaaaaga aacgcccgcg gggcaatgag ggccaggagc tcatggtggt catgtccaaa
    16921 ctgtggctcg cgaatctctg ggcctaaata ccacggggcg tcccagttgg agaaacttag
    16981 cccgacaccg ccctcgctcg aaaccgtggc cctaatccgc tcgtcgaagg ccgctaggta
    17041 gagcacttcc tttccgccca gagaatggcc aatggccccg atgcgttgcc catcaacacc
    17101 tggcaactgg gtcagcaaat caaccgccgc catcccgtcc gctaacatct tgcccatccc
    17161 cttcgaaccg ggaaatgacc gttgatagcg gcgaacctgt gcctggtaat catacggcgg
    17221 tccatcactc cacaaataac aacgcggaca taaggtcaca tatccccgct tggccagttg
    17281 aaggccaaag gccttcgttg tttccgccgt tagcccggcc ggttggcgga ttgtgtcatt
    17341 cgtcgtggag tgaaaaacga tcaccgccgg caaaagggtc ttgttcgatt ctaaaggatt
    17401 ggtgtcctta gggaagagaa gaaacgcctc tgtgggccaa ttacccggcg tttcgtaacg
    17461 aatcaaacac cgcaccacgc catccgtctc cgccttctcc atgacctgca tcgtggccaa
    17521 actgcggtgg atatcacgct caggcaactt cagaaactcc aaccaccact tgcgaatacc
    17581 ttcacgatat gcccgccact gctcggccct cgcaatgcga gagccatccg gcctgacgag
    17641 caagttcatg ggaatgtttt gatcattgcc caaagccagt cccatctgaa catcgccgag
    17701 ccaagggaca tgatgaaccg gcgctacgtc ctggccaaaa cctaccgccg cttcccatac
    17761 cagaaaaaga ccccataaaa ccgtccaaac caaatcgcct ttacgtctgt ctccgctttt
    17821 atcgaaactt ctctcgcacc ggccaaatgg ggctttcacg gctgcgcctc cttaaatagg
    17881 acgacgactc tcgagcatcg gacggcccga aaacatctcg ctggtgccgg agctctcttt
    17941 ccaaggcatc aaccgacgtg ttattcagcg atagctgtgc agaatcttct gacggtaatg
    18001 ataaaatcta gccaaaacga tcttacacca aatctttggc cattcccttt ggcaataact
    18061 gcctacggat atcgcttcac tgggacgtcg tttttttgtt gagggccagg cgtacttcgg
    18121 ccatctcgaa taatcttttc taaaagtttt ttcatggcct caacgcgatc ggaataacgg
    18181 tcggccagat tggcggtttc gcccggatca ttgtcgagct gatatagctc agggtttttg
    18241 ttcaagatta gtttccacgg cccctcgcgg acgctaggga ccccgctgct agcacaactg
    18301 accgcatggg gccggacaga ccgattctct ccccgaaaca gcggcaaaag actgaagcta
    18361 tcctctccag cgttgggtgg gatgggaacc tctaggatct cggccaacgt ggcaaagagg
    18421 tcggcttgat gcaccaattg aatgcacaca ctgccgggcg gcacgacctt cggccagcga
    18481 acgatgaacg gaacacgatg gcccccttcg tacaccgatg ccttatagtc ccggaaaggt
    18541 ccactggaaa aatggcccat cttttccagt gcttcggccc ctgtatagcg ggccttgcca
    18601 ttgtcactgg tgaagatgac aagggttttg tcggtcaccc cgatttcgtc gagggtatcc
    18661 agcacacgcc cgaccatggc atccgtctcc atcacgaaat ccgcatacac gttgaggccg
    18721 ctttttccct tccatggttc attcaccgcc aatggctcat ggggactcgt caaagggaag
    18781 taaagcaaga acggttttcc atcctcccca cattgacgta tgaattcgca tgcccggtcg
    18841 gtaattgttg gaaggatgtc cccaaaacgc cagtcgggca aagcgggtcc cgggaggctg
    18901 gccatccgtg gttcggcgcc gagaaaacgg tcgggaagca gcacgcttgg gattcccacc
    18961 gtccgatcgt ttttgatgaa acaaaacggc ggccagttcg gcacatcggt gccaaagtac
    19021 tcgtcaaatc cccgttccgt tgggccaccc ggtattcgtt gggaaaacac cctctgccaa
    19081 gcttctacct gttccttggt tggtggtggc gtttctttct ggttgcgggc aacacgaaaa
    19141 tacttcatct cctcggcagg gataggccaa tcccagccca aatgccactt accaaaacag
    19201 gccgtgcggt acccacgggc cttggccaac gtgccgatcg tcatccggtc gggagcaata
    19261 agcggttccc caaacacgtt cacaatccct gcttgaagcc gggtccgcca gtgatagcgt
    19321 cccgtcagca gcgtgtaacg ggagggagaa caaacgccac tgctggaatg cgcatcggta
    19381 aatcgcatcc cctctttggc aagccgatcg atgttggggg tcgggatctt tcctcggtcc
    19441 ggattatagc tggatacatc tccgtacccg aggtcgtccg caagaatcac cacaatgttt
    19501 ggattatcga cgccatcggc ggcgaacacg gccccggaca aacagatcgt tatcaggaga
    19561 gcggcggcga gaaggtcaat gcccattcgg aaggcctcgg gccctctatc ccaccgcata
    19621 cgataccgca acttcgacaa acccggcctc atggcgctac ctccccagaa atgcctattc
    19681 caaaatggtc aagaaattcg cctattctgt ttcaagcttc tctcaccaca caaaggccca
    19741 ccgcatctca tcacgcagcg gaaccaacat gatttgggcc gcgtttacgt aatcccgctg
    19801 acggccctct tgaaggagcc cccccgggcc gaatggtaat tcccctcaac ctttaccgaa
    19861 aacaccgatc tgcttgaggc atggttcagt gttgccaaca gcatatccaa acggatacgc
    19921 aaaataaaga gaccggggtt ttcgcctgtg gttgatcctt cagttggcct gtaacaaaaa
    19981 gtatcccaac gccgccggct ttacgacgac catacgcgat gtatggggac gcgccgtggt
    20041 ctccagtgcg ttagcgattg atgcctcatg atttcccagg gcaggacaac gcgtggctct
    20101 ccggggtgag gaacctaagg gaatcacttt ccatacccga cgtgtgaacg ggctatcgcg
    20161 ctgagcgtgc ggcaaacgct gggcttttaa ccgccaaaca attcgccgcg tttccacgcg
    20221 cccgcacgac cgctgtctca catccagcac gttcccgaac ccatttgtga agggcaacat
    20281 ggggttttct ccttagatcg ggacggagat tttcgtggcc gcacggcgaa gtgtttgatg
    20341 agttgtatct cactcgcttc gccgcgcatg aatgtgtccg tcccagtaca ataaaagtgc
    20401 agggtagcag gggagaattg gggtccaaga atgatggttt tctgaacaca tccgacgatc
    20461 atgggccaac ggaaaccctg cgaacgaggt gtgcgtccaa acatctgtgg agcatgtttt
    20521 ttgcaagggt caccatcatg acggcagaca aaggattacc acgaaagtca cggcgaattg
    20581 tccgtcgtcc ggcaacgcga tttccccatc ttttgaaacg catgcggcag cgattgttgg
    20641 accgccccaa gaagggacga gcaacctgag gcaacgcgtg agcgtgtgat ggtgcgttga
    20701 gcaacttggc ggcaacttcg gcaaaggatg atccatggga tatcgaacac tgcgcgagtg
    20761 tgtcaaagat ttgctagcaa cccggcagat gatccgcatc gacgtgccag ttgaccctga
    20821 tcttgaggcc gccgagatcc agcggcgcgt cttccgtgcg ggggggccgg cgctgtactt
    20881 cgcctcggtg cgagggtcgc gcttccccat ggtgagtaac ctttttggga cgatggagcg
    20941 aatccgctat atctttcgcg atacgctgga tcagttgtcc aaactcgtgt cgctgggctt
    21001 ggatccctcc gactggttgc ggcgaccaag gctttttctg aaggtgccgt ggtggggatg
    21061 gaaggcctgg ccgaaggtcg ttagcggcgg cccttgcttg aaacatgaaa tccgattgca
    21121 gcaacttccc aaggtccgtt cctggcccaa tgacggcggg gcgtacatca ccctcccgat
    21181 cgtttacaca gaggacccca cgcaaccagg tttcgcccat tcgaatctgg ggatgtacag
    21241 gattcaaatc accgggggaa aatatgaccc ggagtgtgag gtcggattgc actatcagat
    21301 tcaccgcgga attggcattc atcatgcgcg tgccattgaa gccaagcaga aactccgcgt
    21361 caatgtcttc gtgggtgggg cacctgccat gacggttgcc gccgtcatgc cacttcccga
    21421 agggatgagc gagctattct tcgctgggct tttgggcgga catcgtattc ccatgattcg
    21481 ccggaaaggg gaactcccga tctacgccga ggccgatttc gttctcaccg gctatattga
    21541 acccaagaaa ctactgcccg agggaccttt cggtgaccac ctggggtatt acagccttgt
    21601 gcacgatttt ccggtgatgc atgtagagca cgtgtaccat cgcccaaatc ccatttggcc
    21661 gtttacggtc gtggggcgac caccccaaga ggattcgatg tttgcccagt tgattcacga
    21721 gcttgtcggt cccgttattc ctaaagccat cccaggtgtg cgggccgtcc atgccgtcga
    21781 cgccgccggg gtccatcctc tcttgttggc catcggtagt gagcgatata cgccatacga
    21841 cgaacgaaaa cggccacaag aattgttgac attggccaat gccctgttag gacacggcca
    21901 gttgtcattg accaagtact tgtttattgt cgccggcgag gataaccccg atctggacgt
    21961 ccacgatgtg gatgacttct tccgacatgt tttagaacgg gtggattggc ggcgggatgt
    22021 gcatttccaa acctgcacga cggtggatac gctggattac acaggcgggg cattgaacca
    22081 gggctcaaaa gtgaccattg ccgcagtggg aaaaccgcga cgcacgcttc ccgtcgcgct
    22141 cgattcacga atcaagattc ccgaagacct ggggttttcc gatcctcggg tgatgttgcc
    22201 cgggatcctg gtgattcaag gtcctcccta ccgccgcaac gaaaaagggg aagacgaatc
    22261 gatccggcag ttttgtgcac ggtattctcg gcacgatgcg gtcaatcttt tccctctcat
    22321 tgtgatcgtg gatgatagcg agtttgccgc ccgaacgctg aacaactttc tctggacaac
    22381 cttcacccga agcgatcccg ccggggatat tcacggcatt gaagaattcg tatcgaaaaa
    22441 acattgggga tgccacggtt cgttggtcat tgacgcgcgg gccaagccgg aacacccgcc
    22501 gcctctggtc gaggatcccg aagttgtccg ccgtgtcgag gcgctggccg ccccgggcaa
    22561 accactccac ggcatcatat gagcggtacg tcatttgccg gttgcgtgac agtccaacca
    22621 tccgtaaccg tatggaggat cggtgagcta cgcgagccaa acaagcttgc acgccccaca
    22681 tgttctgtga aaccgatcgc aggaaaagtc tgtctcgggt tgagcgcgcc gtgtaaatcg
    22741 ccttttttga ttacagccac ccggtagatc atcctctgcc gaggatagaa aagaaaaacg
    22801 accgcatcca tggtcttgaa tgcggtcgga atagccggtg gtcaatgata ccggtcttga
    22861 ctcagttctg ccgtgcggcc tctacttcgc cgggcgatgg cgtgcccaca gagcgttcgc
    22921 cactgttcat cacaacccgg ttagcctgag ctaccacctg aattgccgcg cgcatctcct
    22981 caggtgagaa gtacggacgg cgtaacccga tggcagtccg gatttctttt cgggccaact
    23041 cctcgagact caaaccattt tggaaatgcc aagccgcggc ctgggcaacg cgctgcggaa
    23101 tcgttcccga agccagcatc ttcagaactt cattcacctc ggggcacttg gcatacgatt
    23161 cgatgggctt gatctcataa gggatgcgtg gattgggatc tggctttcca tgatcaaggc
    23221 aaacgatggg gatcttcgcc tgaaccacct tctcagcagg gacgttccac atcccgccac
    23281 cataaccacc atagccaccc ccgtatccac cgtaaccccc gccaaagcca ccaccgaatc
    23341 cctgggcacc accgccgtag cctccaccac catagccacc ccgccctcgt ctgccgccat
    23401 agcccatttc acccccaatg ccgccaaatt gtgctagcac cgggacaccg gcaaacgttt
    23461 ctggcatacg gatcgtcaat ggtttgtccg tcttgttctt gacaaccacg ttgcattccg
    23521 tggaatcctt ggggatcagc ttgacctcaa cgagcttctg gtcaatcgcg gcaaacagat
    23581 caatcgtttc cgctgttgca accggagccg gcggattctg attctttacc actggatccg
    23641 ctccgagtaa ccacgtcgcc gaaaggccca aaagaaacgc accgacgaga gcggcgatcc
    23701 gccacatgga acgactcatg gtaatccctc ctcaagtgac ccaataactg ggctcgtctt
    23761 tttcacaaaa cctcttcctc tttcagcgta atcaaaaaaa tcggaaaagc aatgcaaaac
    23821 aacgaaagtt tgccaatttg cacccggctc tcggccaaag ggcctgctta acctttcgac
    23881 gccgaagttc tggtacaagt ttcgcgtgcc ggggttaccc ctctcattgg ttactctata
    23941 taaccctttg gccaggtgta acatgaaggt acccaggatt cattggggac ttccgcatag
    24001 cgtcccttat ggttggggct tgcaaaccgc ttatcaagcg tcaaagataa tgaccggttt
    24061 ggcagtgcat ttattttaga gcccatccca taatctcccc tcgcccgttc ggcgggagag
    24121 ggggcggggg tgagggtggc ttgaatcggt gccggttttc tcgatcgatt ccgatgttcg
    24181 cttgccctca ccccaaccct ctcccggagg gagacggggc tttttgagat gggctcttag
    24241 ccagccaaac aactcatcct tttgggagaa ctcgtgtacc caaggaaatt gtcggcgaaa
    24301 tcgtccaaca cggtaacctt ctactgggag gggaatgcct tgagtcaatc aacgcgtcgc
    24361 acgtttttga aggtctcggc ggggatttct gcttccaccc tgaccgcccg ccaatacgcg
    24421 cgggcatggg aggccaatcg tcgcttggtg attgggatca tcggctgtgg gggacggggc
    24481 ggaggccttg cccaggcatt tgccgagatt tccgacatcg cctatgtctg cgaccccgac
    24541 cgttcccgct gcgagcgctt acagggggca gtgaaagcca agaaagctgt caccgatccg
    24601 cggcaggtcc tcgatgaccg atccgtcgat gccgtggtca tcgcaacgcc cgaccactgg
    24661 cacgcaccct tggccatttt ggtctgtgag gtaggtaaac acgtttacgt cgaaaagccc
    24721 ttgagccata acttccgcga gagtcagctc cttgtcgagg ctgcccggcg ggccagggtc
    24781 gtcgtacagc acggcacgca ggcgcgatcc agccctctca ttaacaaagc cattcaactg
    24841 ttgcacaccg gcgtgattgg agacgtcctg gtagccaagg cgtggaatgt gcaacgccgt
    24901 gtcaacattg ggcacgagca gccctcagaa ccgccgcccg gtgtggatta cgacatgtgg
    24961 gtaggacctg ccgagtttgt gcccttccaa aagaatcgtt tccactacac atggcactgg
    25021 tggtacaact tcggcacggg agacatcggc aacgatggtg tgcatgagct tgattatggc
    25081 cgatggggat tgggggtttc gggtttgccg aatcatgtca tggcagaagg gggaaagtac
    25141 ttcttcgacg atgaccagga gtttcccgac accgtgacct gcgccttcga gtggacaggc
    25201 gacggaaaaa ttggaaacaa acggcaattg attctcgaaa tgcgtatctg gtccaccaat
    25261 tggccgtacg gcgaggaaaa tggcgtcgaa ttttacggca ccgagggccg aatggtactt
    25321 gccaaggaca ggaagataga aatctacgga gaacgtgacc gcttccgcga tgacatcaaa
    25381 ctggatgacc tcccgcagat gccgcccagt catcaagccg attttgtcga ggcgatccgc
    25441 acaggaaaaa agcccaccgc ggatgtcatt gaagggcacc attccgttgc cctaacccat
    25501 ctggccaata tcgctgtgcg tgtggggcgg gctctgcggg tcgatcctca acgggaaaca
    25561 attatcggtg atgaagaagc cagccggctt ttgcgacgaa actaccgaga aggtggccat
    25621 tgggcgattc ccaagggcct gtgatcgagt tgtccaaagc ccacagttag accgaatgac
    25681 atcacgcctt ttggggagat gctgagccgc ttcaattacc gtccaggccc aaccgccccg
    25741 acggcaattg ttcgcaggat gacaatcggg cagcgatatc ctggctttag ggtttgatcc
    25801 gcaaggcact ggccagcttt gttcgatatt catcgagatt gaggggattc cactcgttga
    25861 gccgcgtcac gcctgccggt tgaatcatgt caataaacgg ctggaaacgt accttggcca
    25921 gggagccgag cggttcctta tcgggggccg tgcgggcacg ctccaggata gcataatcca
    25981 cactcattcg accgatgcgg acccgtttga ggagcgtagg gtcatccttg acggcctctt
    26041 cagcagcctg ccatagttgg tcggcctcca ccagcaactc gtcggtcaaa tggttatggg
    26101 tgggtggaat ccagatgcta cagtgaatgt tctctttctc aacacgatcg tggagcagat
    26161 cgatatactt ccgaatcagg ggggcggcgg ctccgtaata tccttcgagg aattcgttca
    26221 tggcaaggtt ctcgtcgtaa tcaggattcc agaggaactt ggccgtcatg taaccaccga
    26281 gggccgccaa ttcgctgttc ggcgtgttgt aggtatcctg ctcaaagata cctttcacgt
    26341 tgtttgccac aaatagtttg atgttatcgt tacggacccg ctggttggga aacggcagat
    26401 aatagtgccg aaaatcggtg acataatccc agacccagag ccggttcccg atcttggccc
    26461 agccttgaag gtcttccaca aatgcccgat tggccggtaa atcgcaagtc gccaaaggat
    26521 gcgaaaagca acattcgatg gaacacagcc ggataatcac attcggtcgg ggacgcattg
    26581 ttttcggtgg tttgcgtgtc cactgatagg ccaaggtctc gatggccttg tctggataac
    26641 gtttttcgac agcttcagcc acgcggttaa ccatttgaag gacgggggcg atttggctct
    26701 cttcggcctt ggcgagggct tgacagtttt cacattcaca atagttgtac caatcgttct
    26761 gagacaccga gaatacaaac gcctccgaat cgttttcgat ggccttgagg atcccttccg
    26821 taacaagccg gataacatcc tcattggtgc aacataactg cgtgcgagtc ttaaggcgtt
    26881 tccccttgac cagggcaaaa tactcggggt gctcgtcaaa atacttctcg ggaggaacaa
    26941 gccgatcgaa ggtgtgaaca aagaaaccgg ccccaaaacg aaccttcccg ccgtgttggg
    27001 gccctaaccg agcagaagat gaattcatcc gattgcgggc acaccaatcc ccgtcaaaac
    27061 agtcgtaggt aaacggctcg cggtattcca acactggaac caccgtttca tcgagcggcc
    27121 caatgactaa ccgatcataa cgcggaatcc ggctgcaatc tggcgtgaac caccggcatc
    27181 cccagtggtc ctccaataac ccatagaccc cgtaaagcgt tccccgtggt tccccgcccg
    27241 cgatgaccag cttcttgttg acggtcttca atgtgtatcc ctcttttcct agctttgccc
    27301 agtcaatctc aaggcccagt tcttgcagcc gtttattatt tccgagaaga atctcacatt
    27361 ccgccgcagg ctggaaattc tcggtgcgaa ttggcagatt ggcccccgtc atctcgccga
    27421 taaatcgggc caattcgtcg gccgcatgcc gaacaggttc ggatgcctgc tctgggatga
    27481 caatgcaata ctccgacttg ccgttctcgg caatcacaag ctcctgagct ggcgatgatt
    27541 gcatcggaaa aagaacaaaa gtcgccaaaa caaaaaacgt agttgtcgag gttcggagca
    27601 tggctgattc ctccttactg gtgagagatc aatctcccgt ggcaggctac atgtgaacac
    27661 cacaagcgac tcgacgccct gcgattcgat gtgaccacaa tgaacgccgg agccattttc
    27721 ggacatccac agtttagcga tctggcaagt gggtttcatc caccggtccg ggacgaacca
    27781 ccttgggggc tgttttgatc cgggggcgga tggccagcca atcaatgcct tgaaactcag
    27841 gatcgggaat ttcgcggatt tcttcttccc cttgataggg acacattgca tccacccagg
    27901 taatgagtcg caaaaggctg atgtcatcaa ccttgacccc gtagtgacga ccgcttgagc
    27961 aaagatcaat gagcggactt ttgaaggaaa gagcgatcat cggcggcggg gttttgtaac
    28021 cctcgggatc gcgggtatca tagccttccg cgattaacat gccagcaatc ccgaaccctg
    28081 gggggaccac atggggtttg cgataaggtt gccccaagtc ggctgaccaa tgggcaaaaa
    28141 gtatggttcg gcgaatccca gaaaacccgg tcgcgcggtc aaatctaaga ccttgaccgc
    28201 ctacccttcg taatatgccg cggcgtcgca acgcctgagc cccatcgtaa gaaggggtgc
    28261 tttttgatcc tctgtgacgg tgatatccgc ttcctccagt cgacctgcgg gcaatcacca
    28321 ggggcgaaac tcggtctcgt gcagaggcat ggccgccgcc accgaatcgt catccccttg
    28381 gccccgtgcc agaacggcga cgaagacctc acgcgcccct gcccgcaaaa gggcctcggc
    28441 cgcggcgtga catgtcgccc cggtcgtcaa aacatcatca actaggagga cccgacgatc
    28501 ccgcaaccga ggatggtatg gcccacggtt agcgcgaacc gcggatgtta actttgcgtg
    28561 ctcgtcctga tcgtggctcg tatcagcagt gggcccctgt cggataatcg cgtggtgcca
    28621 aaatgtcgcc aacttcgacc tccatacgtc gtggatactt acttccacgg aaaaggcgtc
    28681 gcggacattt ttccaccgct ctcggggttt caatgtccgt tgcgggcggg tgtcgcggac
    28741 ccgtcgaaga agagtggttt ccaatgatac gccaaggtac ttagcgatgg tttcggccag
    28801 gatttccggg ctgttaatac gacgacggcg tcgttgtcgc gcaaagagag gaataggaac
    28861 tacacagtcg atcgcaagct cacggagttt ctcgcctctt tcgcagcaaa aaagccgcgc
    28921 catgcaatgc gctagccgtt cgccgctggg atgcttcatc ctgagaatga ccttgcccaa
    28981 tggccccgca tatggcccta acgaaacgac acctttaaac gcaaagcgtt cccgccggca
    29041 ccagtcgcaa ccgtcaggcg agggcgattc gtcgtccacc tggccaccac acttgggaca
    29101 acttatccaa cccgaaaacg aaagaatcgc caaacaatgt ggacagagga aattgctcac
    29161 gccgggctgt ggtggttttc cgccaagcca aagcagatca taagcccgat cgggatgacg
    29221 ccgcaccctc tcctcgagca aaggccgcca ctcggccaag cccgtttcga tagctacggg
    29281 cttcccacag acaacgcaca ccggcggaaa gacgagatca agccagcccg tcaatattcc
    29341 caagcgaaac gatctttcac tgcctctgtg tggctgttgc atatgccaaa tggagaaagc
    29401 ggacatagag cctatcccta atagtccccc tctccctccg ggagagggct ggggtgaggg
    29461 caagcgaacg tcggaatcta tcgagcaaac ccacagcgat tcaagccacc ctcacccccg
    29521 accccctccc gccgaaaaag cgaggggaga gtgtgggata ggctcttacc ccatcatctc
    29581 gggcaaaccc atccggtgta tcactcgcgc cggcctgata ttggccgcgt ctgctttgtc
    29641 attctattac tgaacaacga ctgttgccat gcttggaaga caatgt
//
LOCUS       DAOSUT010000032        29620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1057_length_29620_cov_10.519432, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000032 DAOSUT010000000
VERSION     DAOSUT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29620
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1057_length_29620_cov_10.519432"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..557)
                     /locus_tag="PLD05_04540"
     CDS             complement(138..557)
                     /locus_tag="PLD05_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOP76733.1"
                     /translation="MLDLSSCPCAGVTLDRLIQPAILTVLAEGPRHGYAVAEEINRRM
                     AFGKETPDISGIYRFLKLMEERGLVVSSWDVSHGGPARNCYEITPEGLRCLAQWIKTL
                     ERYRDGISKLLRAARSASKKAESRMKKIRLKQGHRIT"
     gene            825..3659
                     /locus_tag="PLD05_04545"
     CDS             825..3659
                     /locus_tag="PLD05_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76734.1"
                     /translation="MIVWVVAVVALNACGANEAGETGPLTRRVPLEANMLKGETEFVG
                     NVAALVDEQSLVGDPPTGKPVTGWDFDYRYTQTLPHCVLIDLAVERPIARVWFFDTFN
                     VGKLRLEVGEPGKWQEVATVTTERFQSWVPISVDRTCRYLRLSRLDAAANIAEIAIDE
                     YTPAGLAELEARREAARRREAALRAAAEEARHRPRIDLGPPFGELPLVDEINCGSDSQ
                     GHAFSENPVGVSEVRSILGRPCRVLRPLPDRAAYVAYRIGQFKLLRPGGTYVLVIDYP
                     EDAPRHWVIINGGCETRIGFHTGATLPDALHAKYVDSLPESVRLPLSGEYRQWKMVFR
                     LHDRFPQLGTPRGEATRPLLPEDGFPVIIAQFSSPNIPLSQGAAVSRIRLFEVPDETR
                     LECPIRFPPEPLPRRHIFWREEMADGVIASRNEEKRGVRNPLDWYRFKLSTMKFLGIP
                     TYCKDLLEFGACQGWDPTPYGGNRWVHFNDFHKGLWEQIVEMMGQAGKEILPYYEYAG
                     SRGDQGLGYQRRAKPLTRDDAFTHISWVESANADITDPETWGDFRKMLDLTVARLAHK
                     ARFVGVWLRPRGQLPIGFGDATRKRFAEETGLNHTPSREELQSDTILCKQYYDWWFEK
                     RREFLIHVRDYIREHGAQPDAVVLYTSLLAESGPSFPTWDKILVTDDPATWQTLLQTP
                     LYGQGKKWRVLSLSEVVERGLYLEALLSPPLNWGGWEWHHADPAPDPERYASTKGVLM
                     THAFNRFYTVLSPQTFDRFRGPSGLAIVRHYPLNEDMMLDEADKPILDYFTADIEHAG
                     PYCMAEEALAVAHGDPWYIGYLTGNSLARGFPETVRAFNRAFLSLPALPSEVVSGLTS
                     DKDIVVRRIATEGYGTYFALVNTASCDKPRVALRLPEGTYEDTVSGKILRADDVATHD
                     FVPFELWTIRLLPRDSNQSPAVAPSTDTTP"
     gene            4016..5401
                     /locus_tag="PLD05_04550"
     CDS             4016..5401
                     /locus_tag="PLD05_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOP76735.1"
                     /translation="MKWQLWQSALRRFIVTGVVGLTVLCGLWYFGTGLQAEEALPAPA
                     APVQPPAKDVEIKPLEGTPQELLKFLEDLNAQQGPADPQAMMEFQRKRAEVFMQVADK
                     ILSQDKLPAETLDGAFQFKFRGLLVLLQMGDTQARELLPKLAEQLMAKAEEILKGTPT
                     EDAAQRAVQWAMMAPRLGGPDFLPRVIALPKRLEELGFRQLATQLRGGLLMLRLRMGQ
                     AEDRAELAKDVTAFLEDAWTRFSQAEKITHEDVAMVVPVLQYLEFARLDVNLSDYYGR
                     FGGLFAKNADEQIAELGQQLQGISRRLGLVGKKMELAGKTVDGKDFDWSQYRGKIVLV
                     DFFATWCGPCRAEMPNVKENYERFHSKGFDVVAISIDENRADLERYLQENKLPWVVIH
                     NQDPKAQGPANPATYYGITAVPTVMLVDKDGTVLTFDARGPQLGKKLEELLGPPEPPA
                     KDEKQGTTEKQ"
     gene            complement(5555..6391)
                     /locus_tag="PLD05_04555"
     CDS             complement(5555..6391)
                     /locus_tag="PLD05_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOP76736.1"
                     /translation="MLIIQPHYHAIARTTQDYERMASAGVVAVAEPAFWAGFDRRYPE
                     TFIDYFRQISEFEPTRAAQYGIRHFCWVAVNPKEAENPELSRAVLAHMPEFFQKPTVL
                     GVGETGLHKATKNECEIFEAQVDLALRFGQLLLIHTPHLEDKLYGTKRILEILKGFPL
                     DPDRVWIDHVEEHTIRDVKDAGYWVGFTLYPITKCSFKRAVDMLERYGWERTLVNSSA
                     DWGPSDPATLHDCIFEFRRRGHSLQEALEVFYNNPVRFLSQNPKFDLPLVKLETVGPN
                     AG"
     gene            complement(6500..6745)
                     /locus_tag="PLD05_04560"
     CDS             complement(6500..6745)
                     /locus_tag="PLD05_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76737.1"
                     /translation="MLTRSQVVDHSFLELRCYLLEIAATLDRYDRAPEDSAEHADDRW
                     SKIKQALQILTQERAQPDRTEELLMLFSDRSRLDGRP"
     gene            6956..7093
                     /locus_tag="PLD05_04565"
     CDS             6956..7093
                     /locus_tag="PLD05_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76738.1"
                     /translation="MKRTYFARIVGVKLLWKKAIKTQRQEGRGRWSLRIASPARAVER
                     P"
     gene            7090..7896
                     /locus_tag="PLD05_04570"
     CDS             7090..7896
                     /locus_tag="PLD05_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76739.1"
                     /translation="MSYDASAQALRCPFCGSTELKSQPDGVSLTPKYVVPFEVTKDQA
                     RGLLYQRMRSGFFRPSDLVQRSAITEMMAVFVPYWIFSANTHTYWTADTSDVPFHARG
                     EWRPLFGEQRHQHQAIKIVASKVLSWEESEGLGQYDLSRGVPPEQVDLDNVVVEQFAM
                     PRKYARTHARQLIENAEKVYCANSLLRGRFRNLRVNVKIEQMRGEPILLPVWILAYRY
                     RDRVYRFVVNGQTGRAYGAFPTSPWKVLLAVVLFLLAAVIVLALLAQLAG"
     gene            8015..8815
                     /locus_tag="PLD05_04575"
     CDS             8015..8815
                     /locus_tag="PLD05_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOP76740.1"
                     /translation="MNERREIPHPWRLADLTYYDVRQEHYEVAVLPLGATEPHNLHLP
                     YSMDTREGDLVGEMICSAAWRQGAKVILLPTIPYGTETNQRAFPFAMNVNPSTLNALL
                     ADLIETLVHQGILKVVLLNSHGGNDLKGFLREMYGKSPSKLFLCNWYTVFHDVYHQIF
                     NEPDDHAGEMETSLALAYWPELVARRTDGTLLADSGVKQPTRFEAVNRGWVSITRPWH
                     LLTTNAGAGNPHAASVEKGQRVMEILVERLAKFLVELSQSPLDERFPF"
     gene            complement(9725..11062)
                     /locus_tag="PLD05_04580"
     CDS             complement(9725..11062)
                     /locus_tag="PLD05_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76741.1"
                     /translation="MKSKATRRQFLGSSAAGVAAVWLGSQTAVTGQQAAPPSEIVNVG
                     LIGCGGEGRAVATAHSRLGDARIVAVCDVNSKRLADARQQFGGEKIAAYDDYRRMLDS
                     KDVDAVIVATNDHWHVLPTIHACQAGKDVYVEKPLSVFIAEGQAAVKAAEKYKRIVTI
                     GTQQRSWPHYQQAAEMIKEGKLGIITEVRVWDFDCMYPGFGNPPDSDPPPELNWDFWL
                     GPAPKVPYNPNRYNHFYWFFDYGGGWQVDWAVHHYQIVHWFLEAKGPISAAAMGGFYC
                     FENTNTEWPDSFVAICEYGPTPVAPKGFVMQYTFSGAARRQQRSHAKCFYGTKGSMII
                     DRSGFTITYEAGENRVPTEEFTVENVFKKETHVSSLQAHARVFVDCIKSRQKPPADVE
                     VGHQATIPGHLMNISWKVGRKIRWDPEKEEIIGDAEASSLLSKPYREPWKLEV"
     gene            complement(11304..>13220)
                     /locus_tag="PLD05_04585"
     CDS             complement(11304..>13220)
                     /locus_tag="PLD05_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HOP76742.1"
                     /translation="RCLFVLGLIAGIATVGWAEDFRQWSDVTGKFTVQARFLRVDGET
                     VILEREDGVKVGIEIHKLSPQDRQYLQELTSVNPFKVLGEPPKASDSTLFQPLFINQV
                     GTVVIDWGHVRPIPVNPTVGWQLTVTPREAVTIPAKVIGLPPKLDFFEKVSGLIVNST
                     SKTALVQCTTNRPGNPITTNRLILCNLEIGVLGAILISRNETALSVYPARFGDRQYFL
                     LQQNEFGFGNRATLTIAGYPPVASSAKKESAISLSSFSRQLSWAPYEKESGLARDVKW
                     ADFADERTLITCSGTGLILIWNLGNMQPEASLQTTMGLVPAISPDGHWLAFATRDQIG
                     ILDIRKREIIALQNAPREQIFPVLAFSPTGKKLASLINHRNIQIWDVASGKLEADFTL
                     PSGIEFQGKLVFPHDDFVLGGNRYLFHWKSQLFLWDYDNIEAACSLGDVTCAVVTKKR
                     SGTADHLELVTAHIPHAAAQKVLDLALHNPETFAFHKGMPVKLDLSAIPEDAREKVRH
                     TLTQRLKEMGCEVQEEARVTLAAGVGGPKERTVSYWHSGDYKVKEYTLWIRILVDGQV
                     LWETSAGGVPFILLLKPGENVGDKLRELTAKPNYQWFETVTLPQYLQHVPTKGSVALG
                     RGPLTIEPFKGTRL"
     assembly_gap    13221..13320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13321..15017)
                     /locus_tag="PLD05_04590"
     CDS             complement(<13321..15017)
                     /locus_tag="PLD05_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOP76743.1"
                     /translation="MAWSRLRRTEVALRFVIGLVAATVLCFLIQAWNPPPLLRLDAEL
                     ARDSMVGLPAGTLQWHRPLLQDNEPPPQEGSETATPRAWSKLVLRFLGVWTLVVFLFG
                     VGGLYIFFRERRVLVSLNRLLTLLGLVILSVVIARWLAADIPYTECIPLLLLAQTLAV
                     AYHREFALTLGGIVLVLFLLVTGYEVGMGILWASVLSVAILQLDSVRTRTKLLTVSAV
                     AASVGMVVRFLLALNNDVPLDMALLGELWRIALACIVSGFLMHGLLPFIERFFGILTD
                     MSLLELSDLSHPLLRELARRAPSTYNHSINVGAIAEAAADSIGARGLLVRVGAYFHDI
                     GKMLKPNYFIENQREAENRHETLEPTISSLIIIAHVKDGADLARQHHLPEPIIDFIEQ
                     HHGTTLVEYFYERAKEKREGQAENNKVDENNFRYPGPAPQTKEAAVLMLADAVESAVR
                     SLKDPAPSRIEAVVRDIAERKLHEGQFDETNLTLRELRTIEDSLIRSLVAMYHGRIKY
                     PERRTEERRETERRTESSGGAAVGVTKLAGSPKNGQSTPNSEKAVPGESANAVPAGK"
     gene            complement(15097..16149)
                     /locus_tag="PLD05_04595"
     CDS             complement(15097..16149)
                     /locus_tag="PLD05_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOP76744.1"
                     /translation="MSERSIAVVNWETLVGMFGPCDQHVRMIREALGVRVTANDNRIH
                     VSGEASAVSQATEILERLQVMVRRNGPVAAEEVRRLLLEVQTGPIQEDVSPIEVFSGR
                     RVYPRTPGQAHYVKAIQNHDIVFCIGPAGTGKTYLAVAMAVAALREQRIRKIVLVRPA
                     VEAGESLGFLPGDLQAKISPYLRPLMDALREMIDHDTLRRYADEDRIEVLPLAYMRGR
                     TLNEAFMILDEAQNTTVAQMKMFLTRMGHRSKIVISGDTTQTDLPAHTRSGLVDAWTR
                     LRQIRGIAQVELTTADIVRHPLVQAIVDAYEQHPSKRDPKARETTQESLPANQSDTAN
                     DASTSPGEVLNKETTH"
     gene            complement(16496..16846)
                     /locus_tag="PLD05_04600"
     CDS             complement(16496..16846)
                     /locus_tag="PLD05_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76745.1"
                     /translation="MPPIEIVCSNGHRLKVKDEFAGRTGACPICGVKVKVPPKTRPLT
                     EDDVLKFLGDAPPPPTEPEPAAEVPKIVRTDSPARLPKKICEKCHREILAATRICPYC
                     HTYLGFSDIGAGAR"
     assembly_gap    17045..17144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17458..19056)
                     /locus_tag="PLD05_04605"
     CDS             complement(17458..19056)
                     /locus_tag="PLD05_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76746.1"
                     /translation="MATIVSNPNARLADCIPGLERGVRSKSAARVLAAGGWAWEGFLP
                     WAVVLILGLLWAPNPVSADDLLDAIRRQAAEYRQKLENLAQWCESVGLTAEAERTRRW
                     LHPIAEDRLLIPILPQKIGFETPPETFSPDQVEWYRRWVQLRKEQADSLFAFARGAVR
                     QGRAGLALDLAMAALHENPDHEAIRRVFGYQKYSGYWRTSYEVRRLRAGDVWHEQFGW
                     IPKAYVARYEKGERYFNGRWLPAEEEAKLRSTLQTGWVLESEHYRVLTNHSQEEGVKL
                     LKSLEQLNELWSRLFIRFYASEPQVMALFDARSRPAWTPPRQHKVILFRSRDEYNQAL
                     VSVVPQIAITSGMYDSRTQTCYFFAGPQADPTTIYHEATHQMFQEVGKAVSHPGARGN
                     FWIIEAAAMYMETLRTDEDFLVLGGIDTPRLLAARVRFLQDNFYVPLREFVTLGIKEW
                     QSDPRIGALYSQAAGLMQFLMHYDGGRYRDAAVTYLAAVYSGVDTAETLSQVTKRTYA
                     QLDQEYAAYLKSLPALPPMEIVSP"
     gene            complement(19566..21461)
                     /gene="mutL"
                     /locus_tag="PLD05_04610"
     CDS             complement(19566..21461)
                     /gene="mutL"
                     /locus_tag="PLD05_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOP76747.1"
                     /translation="MSRIKRLPQSVINKIAAGEVIERPASVVKELMENSLDAGASRID
                     VAVEKGGVDLIRVADNGHGIHADELELAVASHATSKIETAEDLCHVRTLGFRGEALAS
                     IAEVSHLRIRSRSSDSDMGGELFVEGGVIRQLVPCGCPVGTVVEVRNLFFNTPVRRKF
                     LRSTTTELAHISETVARIALAHPHVHITLMHNDRPVLQLPATDNLLERIAALYGRELA
                     EGLIPVSSEDGPVRLFGYVAHPRFARRHAKMQFLFLNGRPIRDRNLQHALNEAYRGLL
                     TVDRQPIAFLTLEMPPELVDVNVHPTKQEVRFLDGGRLYGQLLGTIRKEFLTTDLITR
                     LPAGTESDQQTADTPHDPRQELAIRRQIVDWAKGELDRQRAAIQTAGVSPLSYPTSEG
                     VSPVGDAPASQNVIAGEQPQAESPLAASQTNTASLGRPSPQGRLPMDQSPPIRALQVH
                     NRYLVTESEDGVVVIDQHALHERILYEQLRQNIAQGRLPSQQLLIPEPVDLTPVEASL
                     ALENRELLAELGLNVEPFGGNTVLVHSYPALLRTMAPGELLRAVIAALGESPRDVDRT
                     ILLEKILHSMACKAAVKAGEQLNPDEINALLEQSRQVLHSHHCPHGRPAMLVFTREEL
                     DRYFHRT"
     gene            complement(21603..23027)
                     /locus_tag="PLD05_04615"
     CDS             complement(21603..23027)
                     /locus_tag="PLD05_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP76748.1"
                     /translation="MTERVTHLERSTKSQDTASAPPVKVLIIDNDQDHAETMAEALER
                     AGCVCRTATSGPEGAKLIREEEFDVIITDLVMNEIDGLGILTLAKQEQPETEVILVTG
                     HGTIQSAVTAMREGAFNYLLKPLDLAQLRAVVQKAAENVRLRRANVELHRRLDETFGF
                     EGIVGNSPQMRAVLEKVRRIAPTNATVLILGETGTGKELIAQAIHQNSPRKNKPFVAL
                     NCAALSENILESELFGHVKGAFTDACSDRVGKFEYAHGGTLFLDEVGDMPMATQIKLL
                     RVLENGEITRVGSNDPIKVNVRVLSATNQDLEALVEAGRFRRDLYHRLNVVSIRLPSL
                     VERSQDIPILTDHFIKTFSKKHGKQIKGMTPAARHRLQTHNWPGNVRELRNVIESMVV
                     VDYDGILDVDDLPEHLAGDNGRQVDPGNVSLAALVGRSMDELEKMFIAETLRATGGNR
                     EEAARMLGIGERTLYRKIKEYGLS"
     gene            complement(22996..23778)
                     /locus_tag="PLD05_04620"
     CDS             complement(22996..23778)
                     /locus_tag="PLD05_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76749.1"
                     /translation="MGGEAMRTRDEPSQKLLDQYTELARLAGALAHEIKNPLSTIRLN
                     LELLAEDFAQPETPRERRILARIEMVQKECDRLQTILEDFLKFTRADRLNLEPANLNE
                     EIRRALRFFAARAKESGIEIIDFLSSDLPTVLLDREAFQAALLNLLINAQQAMPNGGE
                     LVIRTVPSPDGVVIDLIDNGCGMDAETLAHAFDAFYTTKRGGTGLGLPTARKIIEAHG
                     GSISIQSEVGRGTQVTIKLAIPRQIPGEVVTTHDRAGDASRT"
     gene            complement(24010..26199)
                     /gene="pnp"
                     /locus_tag="PLD05_04625"
     CDS             complement(24010..26199)
                     /gene="pnp"
                     /locus_tag="PLD05_04625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOP76750.1"
                     /translation="MKVRVERRIGAQTLWIETGELGKQAAGCCLVGYGDTVVLCAVTT
                     GAPREAVDFFPLSCDYRERYAAAGKFPGGFLKREGRPSPREVLISRLIDRPIRPLFPE
                     GFRDEVQVQAIVMAADRQNDPDVLAMIGSAAALGISPIPFDGPVGSVRVGLIDGQIVP
                     FPTQEMLEKSDLDLVVSGSKEGILMIEGFGKEVPEDVMGDAIMAAYEYVKQVIDLQEE
                     LTGKVQPTKNPFEPPPPSPLYEELKARYYEAFRQAKQTPGKQERAAACEALKQQALQE
                     MIPDPTAEGAIPLSEFERAWHDLERRVVRDLILSGTRPDGRGVKELRPITCKVDVLPR
                     VHGSALFQRGETQAMITVTLGTPRDEQIVDGIIDEYSKKFMLDYYFPPFCVGECRPIR
                     GPSRREIGHGNLAERSVEPVLPDPDVFPYTIRIISDILESNGSSSMATVCGATLGLMA
                     AGVPIRNPVAGISIGLIKESEDNWLLLTDIIGDEDHFGDMDFKVAGTQNGITGIQLDL
                     KVRSINEAIVRATLAQAREARIEILRKMLSVISRPRQDISVFAPRLLRTKIEPDKIGA
                     LIGPGGKTIRGIQEKTRSTIEVEDDGTVIIAASSLEDAQAALKQVEAVTATVEVGKIY
                     DGRVTSIKDFGAFVEILPGKDGLCHISELADEYVANVSDICKVGDTVPVKVIAIDDQD
                     RVKLSRKSALRELAQKREGEGNVEPPPPPPPKRVPPAHRRQDRDRNR"
     gene            complement(26344..26613)
                     /gene="rpsO"
                     /locus_tag="PLD05_04630"
     CDS             complement(26344..26613)
                     /gene="rpsO"
                     /locus_tag="PLD05_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOP76751.1"
                     /translation="MTITKEQKRQLIEQFRTKEGDTGSPEVQIAILTSRINLLTDHLK
                     VHRKDFASRRGMLMMVSQRRRLLDYLKRRAPQRYLEIIQRLGLRK"
     gene            complement(26840..27049)
                     /locus_tag="PLD05_04635"
     CDS             complement(26840..27049)
                     /locus_tag="PLD05_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76752.1"
                     /translation="MTATQGEKKLNSKGRMLLHLAQVKEQLAAKYARLAKTVSSRVRR
                     QIYLNRVKKLTLQAKNARILAEQQG"
     gene            complement(27158..28333)
                     /locus_tag="PLD05_04640"
     CDS             complement(27158..28333)
                     /locus_tag="PLD05_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP76753.1"
                     /translation="MSTQRTWGGGEEQARLIVRGLTARACDVLTAAPADSALASLLRQ
                     LGFPVFSLPHRARGPSAWLALRRAMRWFRPHVLFMNDPHAVIHGLLASLGLPVARIGA
                     RRTVFRLRSGWLYRQMLDGVVCSSLAAAQACRQAGILSQRISIIYDGVDLGRIRQANP
                     IRGRQVLTELARNCGGAPPSGSDQGDPESVHSGNGSPGTRFIVHVGKLTPAKGQDDLI
                     TAFAGLAKDHSDVAVVLIGDGEWRSHLEARMATLGLSNRVFLPGFRSDALDIVAAADV
                     FVFPSREEGLGGALIEALLLGVPVVASSAGGIPEVLNDNPPLGLMVPPADPAVLQGAI
                     KEVLNLPEEQRGALTRRAKAIATMRFSEDQMVSQLAELLHRVVSSKHLSPHSSPAPR"
     gene            complement(28515..28745)
                     /locus_tag="PLD05_04645"
     CDS             complement(28515..28745)
                     /locus_tag="PLD05_04645"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOP76754.1"
                     /translation="MEFTSVGKFLVLIGVFLVIIGGLIWLLGGLPLHLGRLPGDIHIR
                     GERWSLHIPLATCIVLSILLTLVMNIAIRLFR"
     gene            complement(29234..>29620)
                     /locus_tag="PLD05_04650"
     CDS             complement(29234..>29620)
                     /locus_tag="PLD05_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76755.1"
                     /translation="FDYVHAYSLKGGRDVAQLKGSDGDDTFVGWPEWARLSGSNYFLR
                     VKFFEEIQVDPGAGFDSAILYDSPGADTLVATATSLTLSGDFGSISYLYQLQNFEKVQ
                     AKSRGGTNTKSVDPAALTYLLLTGSW"
BASE COUNT         6308 a         8232 c         8327 g         6553 t
ORIGIN      
        1 cgatcgcttg ccctcacccc aaccctttcc cagagggaga gggggacttt ttgggataga
       61 ctctaaatgc cgttccactt ggcgatctgt ttttcaggaa ggctcaacct tacggtgatc
      121 acggtagctt ccctcggtta ggttatccga tggccttgtt ttaacctaat ttttttcatc
      181 ctgctttccg ctttctttga ggcactgcgt gctgcccgca agagcttgct gatcccatcc
      241 cgataacgtt ccagcgtttt gatccattgc gcgaggcagc gtagcccctc aggcgtgatt
      301 tcgtagcaat tcctcgccgg cccaccatgg gacacatccc aggaagaaac gaccagcccc
      361 ctttcctcca tgagtttgag gaagcggtag atgcccgaaa tgtcgggagt ctctttacca
      421 aaagccatgc ggcggttgat ttcttccgcc acggcgtatc catgtcgagg tccctcggcg
      481 agcacagtca ggatggccgg ctgaataagc cgatctaaag tgactcccgc acaaggacaa
      541 ctgctaagat ccaacatagt ccttcctcat atattggagt tattcgaaca atcgcgttat
      601 ttagcgtagt tacccaaaaa gcacgtgtca agcgactgtg tcggcctgca taaggagcct
      661 tgttggctag attgtccaaa gcttctttgg taactagact ttcaaggaat gtcttgcaat
      721 ccctcgttgt tgtcttcatc agggagttca catcaatggg attttgtccg gtatctcgtg
      781 gcttccattt gggtggccag cgaacacgct tggagtgtgt gacgatgata gtgtgggtag
      841 tcgcggtggt ggcgctcaat gcatgcggcg caaatgaagc tggagaaacc ggcccgttga
      901 cgcgtcgcgt gccactggaa gcgaatatgt taaagggcga aacggaattc gtaggcaacg
      961 tggcagcctt ggtggatgaa caatcgctgg tgggcgatcc cccaacgggg aaaccggtga
     1021 cggggtggga cttcgactac cgatacacgc aaacgttgcc ccactgtgtg ctgatcgatt
     1081 tggcagtcga acgaccaatt gccagagtgt ggttttttga caccttcaat gttgggaaat
     1141 tgcggctgga ggttggcgaa ccgggaaagt ggcaagaagt cgccacggtg accaccgagc
     1201 gttttcagag ctgggtgccc atttccgttg accggacctg ccgttatttg aggttatcgc
     1261 ggttggatgc tgcggcgaat attgccgaga ttgccatcga cgagtacaca cctgcgggct
     1321 tggccgaact cgaggcacgt cgggaagccg cccgccgacg agaggcagct ctccgtgccg
     1381 ccgcggagga ggcccgccac aggccccgca tcgatcttgg accacccttt ggagagttac
     1441 ccctggtgga tgaaatcaac tgtggaagtg actctcaggg gcacgccttt tctgaaaatc
     1501 ccgtgggtgt gtcggaagtc cggtcgattc ttggacggcc ttgtcgcgtt ctacgtccct
     1561 tgcccgaccg agccgcgtat gtggcctatc ggattggtca gtttaagctc ttgcggcctg
     1621 gtgggaccta cgtgttggtc attgactatc cggaggacgc tccgcgacat tgggtcatca
     1681 tcaatggagg ttgcgaaacg cggattggtt tccacacggg agctacgctt cccgatgcgt
     1741 tacatgcgaa gtatgtggac tcactaccgg agtcggttcg ccttcccctt tccggagaat
     1801 accggcagtg gaagatggtc ttccgactcc acgatcgctt tccccaattg ggcacccctc
     1861 gcggggaagc gaccaggccc ctcctccccg aagatggctt cccggtcatc attgcccaat
     1921 tttcctcgcc gaatattcct ctgtcgcaag gggcagcggt gagccgcatc cggctgttcg
     1981 aggttcccga tgagacacgt ctggaatgtc ccatccgttt tccaccggag ccgttacctc
     2041 ggcgacacat tttttggcga gaagagatgg ccgacggtgt gatcgcttct cgtaatgaag
     2101 agaaacgtgg agtgcgcaac ccactcgatt ggtaccgctt taagctctcc acgatgaagt
     2161 tcctgggtat tccgacttac tgcaaggatt tgttggaatt cggcgcatgc caaggatggg
     2221 atcccacacc ctacgggggt aatcgctggg tgcatttcaa cgactttcat aagggtcttt
     2281 gggagcagat tgttgagatg atggggcaag ccggaaagga gattctgccc tactatgaat
     2341 acgcaggcag ccggggagat caggggctcg gatatcaacg ccgagcgaaa ccgttgacgc
     2401 gggatgacgc ttttacccac atttcctggg tcgaaagcgc gaatgccgat attacggatc
     2461 cagaaacgtg gggagatttt cgaaagatgc tggacctgac ggtagctcgg cttgcccaca
     2521 aggcacgctt tgtcggcgtt tggttgcgac ctcgtggcca actacccatt ggtttcggcg
     2581 acgcgacacg aaagcgcttt gccgaagaaa ccgggctaaa tcacacgcct tcgcgtgaag
     2641 aactgcaaag tgatacgata ctttgcaagc aatactacga ttggtggttt gaaaaacggc
     2701 gtgagttctt gattcacgtg cgggattaca tccgcgaaca tggcgcccaa ccagatgccg
     2761 ttgtgctcta cacctcgttg cttgcggaat ctggcccgtc ctttccgacc tgggacaaga
     2821 ttctcgtaac tgatgacccg gccacctggc aaacgctgtt gcaaacgccg ctatacggtc
     2881 aaggtaaaaa atggcgcgtt ctttcactct cggaagtggt cgagagaggc ctgtacttgg
     2941 aggcccttct ctcgccccct ttgaattggg gaggatggga atggcatcat gcggaccctg
     3001 ctcccgatcc cgagcgttat gcctcaacaa aaggcgtcct gatgacacac gccttcaatc
     3061 gtttttatac cgtgctctcg ccgcaaacgt ttgaccgctt tcgcggtccc agtggattgg
     3121 ccattgtccg tcactacccc ctcaacgagg atatgatgct tgacgaggcc gacaaaccga
     3181 tcctggatta tttcacggcc gacatcgagc atgcggggcc ttactgtatg gccgaagagg
     3241 ccttggctgt ggcccatggc gacccgtggt atatcggcta cttgacggga aattcgcttg
     3301 cccgcggttt tcccgagacc gtccgcgcgt tcaatcgggc gttcttgtcg ttaccggcgt
     3361 tacccagcga ggtcgtctcg ggtctgacct ccgataagga cattgtggtt cgccgtattg
     3421 cgaccgaggg ctacgggaca tattttgcct tggtaaacac agcaagttgc gacaagcccc
     3481 gcgtggccct tcggctgcca gaaggcactt acgaggatac cgtttcgggc aaaatccttc
     3541 gtgctgacga cgtggccacg cacgatttcg taccctttga gctttggacg atccgacttc
     3601 ttccgaggga ctcgaaccaa tcgcccgcgg tggctccctc aacggacacg actccctgag
     3661 tgtggcatgc atgtcatggg cgatggatag agaagacagt gtttatacct tcctcgggca
     3721 tcatagcgtt gaaatagatt aaagtagtag gccatgcgga cttttgtgat gccatctgat
     3781 cacacctggc aaaaacgatg gcggtgcggc gcatttcatg ttccaaactg ttcgcggtgt
     3841 gcgttcctat atagacagta acttgtgcgg agaataatgc ctctccggta ggttcttacg
     3901 cctaccttgt tttctgccca caactggcat agtcttatag ctgatgaccc cctgggtttc
     3961 ctcgtgtggt tcgagcggat gacttatcgt tccccaattt gaaaggaaag tacgcatgaa
     4021 gtggcaattg tggcaatcgg cgttgcgacg tttcatcgta accggtgtcg tcggtttaac
     4081 cgttctttgt ggcctttggt actttggcac gggccttcaa gccgaggagg cattgccggc
     4141 accagcggct cctgtccagc ccccagccaa ggacgtggag atcaagccgc tggagggaac
     4201 gccgcaagag ctccttaaat tcttggaaga cctcaacgct cagcaaggtc ctgccgatcc
     4261 gcaggccatg atggagttcc aacgaaaacg ggcagaggtc ttcatgcagg ttgcggacaa
     4321 gattttgtcg caggataaac tccccgcgga aacgctggac ggggccttcc agttcaagtt
     4381 tcggggtttg ttggtcctgc tgcagatggg tgatacccag gcccgcgagt tattgcccaa
     4441 gttggccgaa cagttaatgg ccaaggccga ggaaatcctt aaggggacgc cgaccgaaga
     4501 cgctgcccaa cgagccgtgc aatgggccat gatggcgccg cggttgggcg gcccagactt
     4561 tttgccgagg gtgattgctc tccccaaacg attagaagag ttgggatttc gtcaactggc
     4621 gacgcaactt cgtgggggat tgctcatgct ccgtctgcgg atgggccagg cggaagatcg
     4681 ggcggaattg gccaaggatg taaccgcttt tctcgaggac gcttggacgc gtttcagtca
     4741 ggccgagaag atcactcatg aagatgtagc catggtcgtg cctgtgctcc aatacctgga
     4801 attcgcaaga ttggatgtga acctcagcga ctattacggc cggtttggcg gtctcttcgc
     4861 caagaacgcg gatgagcaaa ttgcggaatt ggggcagcaa cttcaaggaa ttagtcgccg
     4921 tcttggtctc gttggcaaga aaatggagct tgccgggaag acggtggatg gcaaagactt
     4981 cgactggtct caataccgag gcaagatcgt cctggtagat ttctttgcta cgtggtgtgg
     5041 gccctgtcga gcggaaatgc ctaacgtcaa agaaaattat gagcgtttcc actccaaggg
     5101 ttttgatgtg gtggccatca gcatcgacga aaaccgagct gatctcgaac gataccttca
     5161 ggaaaacaag ctgccctggg tggtgattca caatcaggat ccaaaggctc aagggcctgc
     5221 caatccggca acatactacg gaataacggc cgtaccgacc gtcatgctag ttgacaagga
     5281 cgggacagtc ctcacgtttg atgcccgcgg tccgcagttg ggcaaaaagc tagaagagct
     5341 tcttggcccg ccggaacctc ccgcaaaaga tgaaaagcag ggaaccaccg agaagcagtg
     5401 attggcttgc gcagtgcata tgagcgagcc gcattaggcc gagacaatca cctggcggtc
     5461 ataaaccgcg tgtaaagaca ggccgcggta atcgcccatt cgtggaagca agggtgggca
     5521 atcacaaggg taatccctgc cgggattccg tctttcaacc ggcgttgggt cccacggtct
     5581 ccaatttgac caatggcaga tcgaatttgg ggttttggct caaaaagcgg acggggttgt
     5641 tgtaaaagac ctcgagcgcc tcttggaggc tatggccacg gcggcgaaat tcaaaaatgc
     5701 agtcgtggag cgtggccgga tcgctgggac cccagtcggc cgatgagttg acaagcgttc
     5761 gttcccatcc atagcgttcc agcatgtcca ccgccctttt gaaggaacac ttagtgatgg
     5821 gataaagcgt aaaccctacc caataccccg cgtccttgac gtcgcgaatg gtgtgttctt
     5881 ccacatggtc gatccaaacc cgatcgggat cgagtgggaa gcctttgagg atttccagaa
     5941 tccgctttgt gccatacagc ttgtcctcaa gatgaggcgt gtgaatgagc aggagttggc
     6001 caaatcgcag ggccaaatcg acctgcgctt caaagatttc gcactcgttt ttggtcgctt
     6061 tgtgcagccc cgtttctccc actccgagga ccgttggctt ttggaagaac tcgggcatgt
     6121 gggcgagaac agcccggctt aactcgggat tttcggcctc tttaggattg acggccaccc
     6181 agcagaaatg gcggattccg tactgagccg cccgcgtggg ttcaaactca ctgatctgac
     6241 ggaaatagtc gatgaatgtc tcaggatacc ggcgatcaaa acctgcccaa aatgccggtt
     6301 ctgccacggc caccaccccg gccgatgcca tgcgttcgta atcctgggtt gtccttgcaa
     6361 tggcgtgata gtgaggctga atgatcagca tagggcatca actcgtttag gggggaattg
     6421 ctctgttcac ctctctggtg tcttggcact acgatcttgc gatccgaaac atgacgatca
     6481 ataactccgg ccttttagct catgggcgac catcaaggcg ggaacgatcg gaaaacaaca
     6541 tgagtaactc ttcggtgcgg tcgggttggg ccctctcttg cgtaaggatc tgcagggcct
     6601 gtttgatctt tgaccagcga tcgtccgcat gctcggccga atcttccgga gcacgatcat
     6661 aacgatcaag tgtcgccgct atttccaaaa gatagcagcg caactccaaa aacgagtgat
     6721 cgacaacttg cgaacgtgtt aacatcgtca ctcccgcatc gcaggactga gtagaagccg
     6781 atcctttctt tccacgcttg tcgccattat aacaaatctg tgcaaggaaa aaagaatgaa
     6841 tgagcacggg attctatcgg agcagccttt cggcccaagc ggttgcgcct tctcatttta
     6901 agaattgctc aaatcatgtt ggaacgtcag tgccctagct gcgcgagcct gcttgatgaa
     6961 gaggacctat tttgcccgaa ttgtgggcgt gaagctcctc tggaagaagg cgatcaagac
     7021 acagcgccaa gagggccgag gccgatggtc tttaagaatc gcttcacctg ccagggctgt
     7081 ggagcggcca tgagttacga cgcctcggcg caggcccttc gctgtccctt ttgcggctcc
     7141 acggaattaa agtcacaacc tgatggcgta agtttgaccc ccaaatacgt cgttccattt
     7201 gaggtcacaa aggaccaggc ccgcgggctg ctgtaccaac ggatgcgatc ggggtttttc
     7261 cgtcccagtg atttggtcca gcgatctgcc attacggaaa tgatggccgt ctttgtgccc
     7321 tattggattt tctccgccaa cacgcatacc tactggacgg ccgacacctc ggatgtccca
     7381 tttcacgccc ggggagagtg gcgaccgctg tttggggagc agcgtcatca gcatcaggcg
     7441 atcaagatcg tggccagtaa ggtcctttcc tgggaagaat cggagggcct gggacagtac
     7501 gatctttcgc gcggtgttcc gccggaacaa gtcgatcttg acaatgtcgt tgttgaacaa
     7561 ttcgctatgc cgcgaaagta tgctcgcacc catgcccggc agttgatcga aaatgccgag
     7621 aaggtctatt gtgcaaactc gttgcttcgc ggtcgatttc gcaatcttcg cgtgaatgtc
     7681 aagatcgagc agatgagagg ggagccgatc ctccttcctg tttggatttt ggcttatcgc
     7741 taccgggatc gtgtctatcg ttttgtggtc aatggtcaaa ccggtcgtgc ctatggtgcc
     7801 tttccgactt caccttggaa ggtccttctg gccgttgtgc ttttccttct ggccgctgtg
     7861 atcgtgttgg cactgttggc acagctggcc ggttaatttc agcacaggcc gcgagccatc
     7921 ggttcattgt tcggcgtcct tgttcggcga tgacgccgag gcagtaaaat ggtccgtagg
     7981 tcattttcgc ggttcagtgg caagggataa tcgcatgaac gagcggagag agattcctca
     8041 tccttggaga ttagcggatt tgacatacta tgacgtccgt caggaacatt acgaggtggc
     8101 cgtgttgccg cttggggcga cggagccaca caacctccat ctgccgtaca gtatggacac
     8161 gcgggaggga gacctggtag gcgagatgat ctgctcggcg gcgtggcgtc aaggggccaa
     8221 ggtcattttg ctgccgacta tcccctatgg gactgagacc aaccagcggg cgtttccttt
     8281 tgcgatgaat gttaacccca gtacccttaa cgccttattg gcggacttga ttgagacctt
     8341 ggttcatcag ggaatcctca aggtggtcct gctcaacagc cacggcggca atgacttgaa
     8401 aggctttttg cgagagatgt atggtaaatc cccatccaaa ctctttcttt gtaattggta
     8461 caccgttttt catgatgtgt atcatcaaat cttcaatgag cctgatgatc acgccggcga
     8521 aatggaaaca tcgttggcct tggcgtattg gccggagttg gttgcacggc gaactgatgg
     8581 gacacttctc gccgatagtg gtgtgaagca accaacgcgt tttgaggccg tcaatcgtgg
     8641 ttgggtcagt atcacgcggc cgtggcatct tttgacgacc aatgccggcg caggaaatcc
     8701 tcacgcggca tcggtggaaa aaggccaacg ggtgatggag atccttgtcg aacggctggc
     8761 caagttcttg gtggagcttt cccaatcgcc gctcgatgag cgatttccgt tttgaaaatc
     8821 ctgtggctct ttaaaaaatc ggccagaaaa aatgctgcga accgcaccgg cattacttgt
     8881 gtcagacttg aatgtagggg ctaggcaaga tgttgtaacg aaatcgtttt tagtgcgtca
     8941 atttaaagag agtgtgtggc aggcgaaaag aggtcgcaag atgcacgttc gtaggttcgc
     9001 gtaaaggtga atgcctgtag cggttgagag gtcatgtttt tcgtatatgg ccttcgaaaa
     9061 tgggtacgtt cgatacccga atgcgacgag aaatatatga cttatgttat ttcaggataa
     9121 tgacttacgc cataattcga atggtacgct tgcccttggc gtgaattggg tttgactttt
     9181 ttacaggcgt gtgtatatcg tatactgtgg cgaccggaag gttgccaagg ctggagcgaa
     9241 aagcgttagc ctctttatgg gaggacagcc tatgtgccga gcacgagcgc gatctgatcc
     9301 tggcacgcgg gcgtccggcg gtggagtcgg gcgcccgaac aaatggtgaa gatcccgtgg
     9361 tcttttctgg tgaaatggca caggagggtc tatcgagaaa gtgacacaga ctttctgccc
     9421 gggaggacct tcggtgttct gagaggcgtt aaatctggac agtcggtccc gaaaagagtt
     9481 gattcaccct gtcccctccg cgagggaaag ccgcttggca aaccgagtca gccttttttc
     9541 ggccgactga gatagcgtgc tcgggcacgt cagccgccaa atgatggttt caccggagaa
     9601 gtcctctcta aaaagacgac taagacgaaa acgatcggac ggcctcgaca tgaggccgtc
     9661 cgcttttttt ggtacggctt ctaccaatca gccggaagag tcgctgactt ctcccacggg
     9721 tggatcagac ctccagcttc cacggttctc ggtagggttt gctcaatagg ctgctggcct
     9781 cggcatcgcc gatgatttct tccttttcgg ggtcccagcg aatcttgcgt cctactttcc
     9841 acgaaatgtt catcagatgg ccggggatcg tcgcctgatg gcccacctcc acgtccgccg
     9901 gcggcttttg ccgactcttg atgcaatcga caaagaccct ggcgtgggct tggaggctag
     9961 agacgtgcgt ttcctttttg aacacattct ccacggtaaa ctcctccgtg ggcacacggt
    10021 tctcgccagc ttcgtaggtg atggtgaacc cactgcggtc gatgatcatg gatcctttcg
    10081 tgccatagaa gcacttggcg tgggaccgtt gctgtcggcg ggccgcaccg ctgaaggtgt
    10141 attgcatgac gaaacctttc ggggccacag gcgtgggacc atattcacag attgccacga
    10201 acgagtcggg ccactctgtg ttcgtgtttt caaaacaata aaacccaccc atggccgcgg
    10261 ccgagatcgg tcccttggcc tccaagaacc agtgaacgat ctggtaatgg tgaaccgccc
    10321 aatcgacctg ccagcccccg ccgtagtcaa agaaccagta gaagtggttg taacgattgg
    10381 ggttgtaggg aaccttgggc gccggaccta accagaagtc ccaattcaat tccggtggcg
    10441 gatcgctgtc gggcggattg ccaaagcccg gatacatgca atcaaagtcc cacacccgaa
    10501 cttccgtgat gatccccaat ttgccttcct tgatcatttc ggcggcctgt tggtagtggg
    10561 gccaactccg ttgctgcgtg ccgatggtca caatccgctt gtatttttcc gcggccttca
    10621 cggcggcctg cccttccgca atgaagacac tgaggggctt ctcgacatag acatccttac
    10681 cggcctggca ggcgtgaatc gtcggcaaga catgccagtg atcattggtc gccacaatga
    10741 cagcatccac atccttactg tccagcatgc gccggtagtc atcgtaggcg gcaatctttt
    10801 ctccaccgaa ctgttgtcgg gcatcggcca gacgcttgga gttgacgtca cagaccgcga
    10861 caatccgggc atcgcccaac cgggaatgtg ccgtagccac ggcccgccct tcacctccgc
    10921 agccaatcaa gccgacattg acgatttcac tgggcggtgc cgcctgctga cccgtcaccg
    10981 ccgtttgact tcccaaccag acggcagcca caccagccgc cgaactcccg aggaattgac
    11041 gccgcgtcgc cttgcttttc atagggttct tcctccttct tggcaacggt taaagaacat
    11101 accttcaggc tgacaacgaa cgtccgcacc aaacatcagg gccgaacgtt ctggcgggac
    11161 ctgtggcaag aggtcctcgt caaaatgacc cgctgggagg gtgtgccaca attattagca
    11221 aaccgattgc ctcggtcaaa ttgggccaaa ctcgccacga agaattgggg cgcatctcct
    11281 ccgccccgtg gagcatggag cgactacagc ctcgtccctt tgaacggttc aatggtcaat
    11341 ggaccacgtc ccaaggcgac cgagcctttg gtgggaacat gttgcaaata ttggggcaaa
    11401 gtcactgtct caaaccattg ataattcggc ttggccgtca actcacggag tttatctccc
    11461 acattttcgc caggtttaag aaggaggata aacggcacac cacccgcgga ggtctcccac
    11521 agaacttgac cgtccaccaa gatgcggatc cagagcgtat actctttgac cttgtaatcg
    11581 cccgagtgcc agtatgagac cgttcgttcc ttagggccgc cgacgccggc cgccagtgtg
    11641 acccgggctt cctcctgaac ttcgcaaccc atttccttca gccgttgggt gagtgtatgg
    11701 cgaacctttt cgcgggcgtc ctccgggatt gccgacaggt caagtttgac gggcatacct
    11761 ttgtggaagg caaaggtctc gggattgtgt aacgcaagat cgagaacctt ttgggcagca
    11821 gcatggggga tatgggccgt gaccaattca aggtgatctg ccgtccccga gcgtttttta
    11881 gtgacaacgg cacatgttac atcgcccagc gagcatgcgg cctcgatatt gtcgtaatcc
    11941 cagaggaata gttgcgactt ccaatggaaa agataacgat tgccgccgag gacgaaatca
    12001 tcgtggggaa aaacgagttt cccttgaaat tcaatgccgc ttggcaatgt gaagtcagcc
    12061 tccagttttc cgctggccac atcccaaatc tggatgtttc gatggttaat caaagaggct
    12121 agcttttttc ccgtcgggct aaaggcaagg acgggaaaaa tttgttcccg aggggcattt
    12181 tggagggcga tgatctcgcg cttgcggata tcgagaattc cgatttggtc cctagttgca
    12241 aaggccagcc agtgtccatc cgggctaatc gcgggaacga gacccatggt cgtttggagg
    12301 ctagcctcgg gctgcatatt gcccaggttc caaatgagaa tcaatcctgt tccgctgcac
    12361 gtgatgagcg tgcgctcgtc ggcaaaatca gcccatttca cgtcccgggc taggccgctt
    12421 tctttttcat acggggccca gctcaactgg cgactgaagg acgaaaggga gatggcgctc
    12481 tcttttttgg cgctgctggc cacgggagga tagcccgcaa tcgtcaaggt cgcgcgatta
    12541 ccaaaaccga actcattttg ctgaaggagg aaatattggc gatcaccaaa acgcgccgga
    12601 tagaccgaca acgcggtctc gttacgcgag attaggatag ctcccaagac cccgatttcc
    12661 aaattgcaga ggatgagccg gttcgtagtg atggggtttc cgggacggtt cgtcgtgcac
    12721 tgcaccaggg ccgtcttgct ggtggaattg acgattaagc cggacacctt ctcgaaaaag
    12781 tcaagtttcg gcggaagccc gattaccttg gccgggatag tgacggcttc ccgaggtgtc
    12841 acggttagct gccaccccac agtcgggttg acagggatgg gtctgacatg accccagtcg
    12901 atgaccaccg taccgacttg gttgataaaa aggggttgaa atagcgtgct atctgaagct
    12961 tttggaggtt ctcccaagac tttgaagggg ttgacgctgg tcagctcttg gaggtactgg
    13021 cgatcttgtg gacttaactt gtgaatttcg atgcccacct tgacaccgtc ctcacgctcc
    13081 aggatgacgg tctcgccatc taccctgaga aaccgagctt gaacagtaaa tttgccggtg
    13141 acgtccgacc attgccggaa gtcctccgcc cagcccacgg tggcgatacc tgcgatcagt
    13201 cccagcacga agagacaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 tctttgcccg cggggacggc gttagcagat tcgccgggaa cagctttctc gctatttgga
    13381 gtggactgcc cgtttttggg cgatcctgcc agtttcgtca cccccaccgc cgcaccgccg
    13441 gacgattcag tccgccgttc tgtttcgcga cgctcctcgg ttcgccgctc tgggtatttg
    13501 atgcgcccgt ggtacatggc caccaacgag cggatgagac tatcctcgat ggtccgcaac
    13561 tcgcggagcg tgaggttggt ttcgtcgaac tggccttcgt ggagctttcg ctcggcaata
    13621 tcgcggacga cggcctcaat ccgcgaaggg gccgggtcct tgaggcttcg cacggcactt
    13681 tccacggcgt cggccaacat gaggacggcg gcctccttgg tctgaggtgc tggcccagga
    13741 tagcggaaat tgttttcatc gaccttgttg ttttccgcct gtccctcccg tttttccttt
    13801 gccctttcat aaaaatactc gacgagtgtc gtcccgtgat gctgttcaat aaagtcaatg
    13861 atgggctcgg gcagatgatg ctgccgggcc agatcggccc catctttgac gtgggcgata
    13921 ataatcaggc tgctgatcgt cggctcgagg gtctcgtggc ggttttcggc ttccctttgg
    13981 ttttcaatga aataattggg tttaagcatc ttaccgatat cgtggaaata cgcccccaca
    14041 cggacgagca agccccgggc gccgatagag tcagccgccg cctcggcaat cgctccgacg
    14101 ttaatcgagt gattataggt gctgggggca cgtcgtgcca gttcgcggag cagcggatgc
    14161 gagagatcgc tcagttccaa aaggctcatg tcggtgagaa tgccgaagaa ccgctcgatg
    14221 aacggaagca gtccgtgcat gagaaaaccc gacacaatgc acgccagggc aatccgccac
    14281 aattcaccca agagggccat atcgagcggg acatcattgt tcagcgcgag caggaaacgg
    14341 acgaccatcc ctacgctggc cgcgactgca ctgacggtga ggagtttcgt gcgtgttcgg
    14401 accgaatcga gttgaaggat ggctacagaa aggacgctgg cccaaagaat gcccataccc
    14461 acttcgtagc cggtgacaag cagaaatagc accaagacga tgccaccgag cgttagggcg
    14521 aattcgcgat gataagcgac ggccaaggtc tgggccaaaa gcaaaagggg gatgcactcg
    14581 gtgtagggaa tgtcggcggc cagccaacgg gcaatcacaa cactgagaat cacaaggccg
    14641 agaagggtga gcagtcggtt gaggctaaca aggacccgcc gttcgcggaa aaagatatac
    14701 aaccctccca ccccgaataa aaacaccacc aaggtccaca cgcccagaaa tcgcaggacc
    14761 aatttggacc aggcccgggg cgtagccgtt tctgatcctt cctggggagg tggctcattg
    14821 tcctggagca aagggcgatg ccactgaagc gtgccggcgg ggagaccgac catgctgtcg
    14881 cgcgcgagtt cggcatcgag ccggagcagg ggtggtggat tccacgcctg tatcaagaaa
    14941 cacagcactg tggcagccac caggccgatg acgaaccgca gggccacttc ggtgcggcgg
    15001 aggcggctcc atgccacttc ccactttccc ggtaccctta ccaggccggc cagtctctcg
    15061 ctccgcttgc gttttgttcc tccaaaaaac atgaggttaa tgagtcgtct ccttgtttaa
    15121 gacctcaccg ggcgaagtcg acgcatcatt ggcggtatcg ctttggttcg ccggtaggga
    15181 ttcctgtgtc gtctcccggg ccttggggtc ccgttttgac ggatgttgct cgtaggcatc
    15241 gacgatcgct tggaccaaag gatgccgaac aatatccgcc gttgtcaact cgacctgggc
    15301 aattccacga atctgacgca ggcgggtcca ggcgtcaacc agaccactgc gagtatgggc
    15361 cggaagatcg gtctgtgtcg tatcgccaga aatcacgatc ttcgagcgat gccccatccg
    15421 cgtgaggaac atcttcatct gggcgacggt tgtattttgg gcctcgtcga ggatcataaa
    15481 ggcctcattg agtgtgcggc cccgcatgta ggccaggggg agaacctcga tccgatcctc
    15541 atccgcgtac cgtcgcagcg tatcatggtc gatcatttca cggagggcat ccatcaaggg
    15601 gcgaagatag ggcgaaatct tggcctggag gtcccccggc agaaagccca aactctcgcc
    15661 ggcctccacg gccgggcgaa caagaacgat cttccgaata cgttgttcac ggagggcagc
    15721 cacggccatg gccaccgcca aataggtctt gcccgttccc gccggaccga tacagaaaac
    15781 aatgtcgtga ttctggatcg ccttgacgta gtgggcctgg ccaggcgtcc ggggatagac
    15841 gcggcgtccg ctaaagacct cgatcggtga gacgtcctcc tgaatcggcc ccgtttggac
    15901 ctcgaggagt agtcggcgaa cctcttcggc ggccactggc ccgttgcgcc ggaccataac
    15961 ttgaagccgc tccagaatct ccgtggcctg gctgacggcc gaggcttcac cggacacgtg
    16021 aattcggtta tcgtttgccg tcactcgcac acccagggcc tcgcggatca tgcggacatg
    16081 ctgatcacac ggcccaaaca tcccgacaag ggtctcccag ttgaccactg caatggatcg
    16141 ttcactcatc aacttctgtt gacacctcca cgttttacct caaccaaatc ttgatcacgc
    16201 gacttgccgc ctaagggcga cagatggttc atcgtgcaga tttcttaaga ttgtactata
    16261 accaatcata tgcatcacgg gcttgttgaa tctgctcctt cctcggacaa gtagcaaaaa
    16321 gaaacgcatg attttcggca caggacttgc acgggaataa gcttacggga atgagaaaag
    16381 aataagcccc atcgctccgc gggcccctat tctccttccg tcggccgaca gcctctctta
    16441 atacctgacc gggacggacc gccgctcgtg aatccggcaa gacaattctc atacatcatc
    16501 gggcaccagc cccaatatcc gaaaagccaa ggtaggtgtg gcaatatggg caaattcggg
    16561 tggcagcgag gatctcgcga tgacatttct cgcagatctt tttcggtaat cgggcagggg
    16621 agtccgtgcg aacgatcttg gggacctcgg cggctggttc cggttcggtc ggcgggggtg
    16681 gcgcgtctcc taaaaacttt aaaacatcgt cttcggtcaa cggacgggtt ttagggggga
    16741 ccttaacctt aacaccgcaa atcggacagg cacctgtcct ccccgcgaac tcatctttga
    16801 ccttcaaacg gtgtccgttc gaacaaacga tttcgatcgg tggcataact gttcccctct
    16861 gtagtgggga aaggggcaca ttgattcggc caattcatcc tcatcatcat ccttctctac
    16921 atccgcgatc attccttcgt cgcacctgcc gggccctgct gggtgacgcc gaaccgccca
    16981 cggtcagcgg ttcactcagc aatgccttgc cgcaaggagg ggcgacagct ggcccgcttg
    17041 ataannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagccaa caaggcgtcc
    17161 gtttttcaag attacaaacc aaacgaacgg tgtgcttatc gaaaaccgtc gtccgtgttc
    17221 gggctattcg gcgaatagca gagagagcgt ttcttcaggt tgtccgtgct ccggcactcc
    17281 gctattacaa acttgatttc tgctattgca ggcatacagc cagcgccggc cttacgacaa
    17341 cccacattgt attttaccca cagtgcatcg tgtgtcaaaa tgatcgcttt ggttttgttt
    17401 cgtttctggc attaaggtgg gaaattttct tggcatgccc cgtcgaaagg cgtgttttca
    17461 gggactcaca atttccatgg gtggaagggc tggtaaagat ttcagataag ctgcgtattc
    17521 ttggtcgagt tgggcgtagg ttcttttcgt cacctggctg agtgtctcgg ccgtatccac
    17581 gcccgaatag acggccgcca aatatgtcac ggccgcatcg cggtagcgac caccgtcata
    17641 atgcatgagg aactgcatca agcccgccgc ttggctataa agcgcgccga tgcggggatc
    17701 gctttgccac tccttgatcc cgagggtgac aaactcccgg aggggcacat agaaattgtc
    17761 ttggagaaag cggacgcggg cggccaagag ccggggcgtg tcgattcccc cgagaaccag
    17821 aaaatcctcg tccgtgcgga gcgtttccat atacatggcc gcggcttcga taatccagaa
    17881 gttacctctt gcgcctgggt gactgaccgc tttgcccact tcctgaaaca tctggtgcgt
    17941 ggcctcgtgg tagatcgtcg tgggatccgc ctgcggaccg gcgaaaaagt agcaggtctg
    18001 ggtccggctg tcgtacatgc ccgaggtaat ggcaatttgt gggaccaccg acacgagggc
    18061 ctggttatat tcgtcccgac tgcgaaagag aatgaccttg tgttggcggg gaggtgtcca
    18121 cgcgggacga gaacgggcgt caaacaaggc catgacctgc ggttcgctgg cgtagaaacg
    18181 aatgaataag cgactccaca actcgttgag ttgttccaac gacttgagca gctttacccc
    18241 ctcctcctga ctgtgattgg tcagcacacg ataatgctcg ctttccagca cccaaccagt
    18301 ctggagcgtg gagcggagtt ttgcctcctc ctcggcggga agccagcgtc cgttgaaata
    18361 gcgttcgcct ttctcatagc gggcgacata cgccttggga atccagccga actgttcatg
    18421 ccacacgtcc cctgcccgga gccgccgcac ttcataactc gttcgccagt agcctgaata
    18481 tttttgatac ccgaataccc gccgaatggc ctcatgatca ggattctcat ggagggcggc
    18541 catggcaagg tcgagggcca atcctgctcg accttgacga acagccccgc gggcaaaggc
    18601 aaagaggctg tccgcttgtt ccttgcggag ttgaacccaa cgacgatacc attcaacctg
    18661 atccggcgaa aatgtctccg gtggggtttc gaacccaatc ttttgcggca agatcggtat
    18721 gagaagtcgg tcctccgcaa tcgggtgcaa ccaccggcgg gtccgctcgg cctccgcggt
    18781 aagaccgacg gattcgcacc actgcgctaa gttttctagt ttctgccggt actcggcggc
    18841 ctggcgccgg atggcatcga ggaggtcatc cgcacttacg gggtttggtg cccacaatag
    18901 gccaaggatt aaaacaaccg cccagggaag aaaaccttcc cacgcccatc cgccggccgc
    18961 caaaactctc gccgcactct tgcttcggac gcccctttcc aagccgggaa tgcaatctgc
    19021 caatcgagcg ttgggatttg agacaattgt ggccaaccta acccctcctt gccgaaggta
    19081 tcaattctca cgaataagcc gcgacgatcc tatcagttat gacgtttttg cagggaatta
    19141 gtttcctggg taactgagag gcggccccat caacacgatc atccgccgtc tcttttctcc
    19201 cttatttgag cttaccacac cattgtgggg aacgccgtta aataggtaac cccgataagt
    19261 gagtaagcga aaagcaggga aagactgccc taagaaggta tcctatactc tcccctcgcc
    19321 cgtttggcgg gagaggggtc gggggtgagg gtggctcgaa tcgctggtgg ttttccgatc
    19381 aactccgacg atcgcttgcc ctcaccccaa ccctctcccg gagggagagg gggacttttt
    19441 gggatggact cttagccacg cgattccccg accgtttcgt cccatcggca gcggtgggcc
    19501 caccgacgag ttgttctcat gcacgatctt ctcatccaca atcgcgcgcc ggccaaaaaa
    19561 aggacttatg tcctgtgaaa ataacgatca agctcttccc gcgtgaagac aagcatggcg
    19621 ggccggccat ggggacagtg atgcgaatgc aacacctggc ggctctgttc cagtagggcg
    19681 ttgatttcgt cgggattgag ttgctctccc gccttgacgg ccgccttgca cgccatcgag
    19741 tggaggatct tttccaggag gatcgtccga tcgacatcgc ggggtgattc tcccaaggca
    19801 gcgatgaccg cccggagcaa ttcccccggt gccatggttc gcagcaaggc ggggtaactg
    19861 tggaccaaca ccgtgttgcc gccaaagggt tccacgttga gtcccagttc ggccaaaagc
    19921 tcgcggtttt ccagggccag actggcttca acaggtgtca gatcaaccgg ctcagggatg
    19981 aggagttgtt ggcttggaag gcgtccctgg gcgatatttt ggcggagctg ttcgtagagg
    20041 atgcgttcgt ggagtgcgtg ctgatcaatc accacgaccc catcttcgct ttccgtgacg
    20101 aggtaccggt tatggacctg gagcgcccgg atgggcggcg attgatccat gggcagacgt
    20161 ccttgcggtg acggtctgcc aagcgaagcc gtgttggttt gtgaggcggc aagtggcgac
    20221 tccgcttgag gctgctcccc cgctatcaca ttttggcttg ccggtgcgtc acccacaggc
    20281 gaaacgcctt ctgatgtggg gtagctcaac ggagagacac ccgctgtctg gatggcggca
    20341 cgctggcgat ccaattcccc tttggcccaa tccacgattt ggcgacggat ggcgagctct
    20401 tgtcggggat cgtgtggggt gtcggccgtc tgttggtcgg attccgttcc ggcggggaga
    20461 cgcgttatta aatctgtggt gaggaattct ttgcggattg tgccgagcaa ctgcccgtac
    20521 agccgacctc cgtctaagaa tcgcacctcc tgcttggtgg gatggacgtt gacgtccacc
    20581 aattccggcg gcatctccag cgtgaggaat gcgatgggct ggcgatcgac ggtcaacagg
    20641 ccgcgatagg cttcgttgag ggcgtgctgg aggttccggt cgcgaatggg acgcccgttg
    20701 agaaaaagga attgcatttt ggcatgccgg cgggcaaacc gagggtgagc cacatagcca
    20761 aacaggcgga ccggaccatc ttcgctagaa accgggatca atccctcagc caactctcgc
    20821 ccgtagagtg ccgcgatccg ctccaggaga ttatccgtcg cgggcagttg aaggacgggg
    20881 cgatcgttgt gcatcagcgt aatatggacg tgaggatgtg ccagtgcgat gcgggcaacc
    20941 gtctcggaaa tgtgcgccaa ttctgtcgtt gttgagcgga ggaacttgcg acggacaggt
    21001 gtgttaaaaa agaggttgcg gacctcgacg accgtcccca cggggcagcc gcaaggaacg
    21061 agctgacgga tgacaccgcc ttccacaaaa agctcacctc ccatgtcgga atctgagcta
    21121 cggctccgaa tccggagatg tgagacctca gcgatagaag ccaaggcctc gccccgaaaa
    21181 ccgagcgtgc gaacgtgaca gaggtcttcc gcagtctcga tcttgctcgt ggcgtgactg
    21241 gcgaccgcca gttccaattc gtcggcgtgg ataccgtgcc cattgtcggc cacgcggatt
    21301 agatcgacac cgcctttttc cacggcgaca tcaatacggg atgcccctgc gtcgagggaa
    21361 ttttccatca attccttgac cacgctggcc ggtcgctcaa tgacctcgcc cgccgcgatc
    21421 ttgttgatca cactttgggg aagccgtttg attcgcgaca tggacgttcc ttacactccg
    21481 cagggccgat ctttcaaaga agacgctctc aatgttgttt ggcttgaatc ctgaattcac
    21541 atcaaccaaa caaagccccg accgtgcgaa aagcctccga gtgcggggaa ggctctccct
    21601 gcttatgaga gaccgtactc tttgatttta cgatagagcg tgcgttcgcc aatgcccagc
    21661 atccgggcgg cctcctcgcg gttgcctccc gtggcccgga gcgtctccgc aatgaacatc
    21721 ttttccaact cgtccattga tcgccccacc aaggccgcca gcgacacgtt gccggggtcc
    21781 acttggcggc cattatcccc ggccaaatgt tcgggcaggt catccacgtc gagaatcccg
    21841 tcgtaatcca caacgaccat gctctcgatg acatttcgca attcgcgaac gttgcccggc
    21901 caattgtggg tttgcaagcg atgtcgtgcg gccggcgtca tccccttgat ctgtttgccg
    21961 tgctttttcg aaaacgtttt gatgaagtgg tcggtgagaa tggggatgtc ttgggacctc
    22021 tcgacaagac ttggcaagcg gatgctgacg acattcaagc gatgatacag gtcgcgacgg
    22081 aagcggccgg cttcgacaag tgcttccagg tcttgattgg tggccgagag aacgcggacg
    22141 ttcaccttaa tcgggtcatt tgagccgaca cgagtgattt caccgttctc caagacgcgg
    22201 aggagtttga tctgagtggc catcggcatg tcaccaacct catcgaggaa gagcgtgccg
    22261 ccgtgggcgt attcaaactt gccgacacga tcagagcagg catcggtaaa cgcccccttg
    22321 acgtggccga acaattcgct ctccaggata ttttcactca acgcagcgca attgagggcc
    22381 acgaaaggct tgtttttgcg agggctgttt tgatgaatgg cctgggcgat caactccttg
    22441 cccgttccgg tctcgcccaa aatgaggacc gttgcgttgg tcggggcaat acggcggacc
    22501 ttttccaaaa cggcccgcat ctggggactg ttgccgacaa tcccctcaaa accgaacgtt
    22561 tcatcaagcc gccgatggag ttccacattg gcccggcgta agcggacatt ctccgcagcc
    22621 ttttgaacga cggcacggag ttgggccaaa tcgagtggct tgagcaaata gttgaaggca
    22681 ccttctcgca tcgcggtaac ggccgattga atcgtcccgt ggcccgtcac gaggatgact
    22741 tctgtttcgg gttgctcctg tttggccagg gtcaaaatcc ctaggccgtc gatctcgttc
    22801 atgaccaaat cggtgatgat gacatcaaac tcttcctcgc ggatgagctt ggccccttcc
    22861 gggccggatg tcgccgtacg acaaacgcag ccggcgcgtt caagggcttc ggccatagtc
    22921 tcggcgtgat cctgatcgtt gtcgatgatc aggaccttca ccggcggtgc ggatgccgtg
    22981 tcttgtgatt tcgtgctacg ttcgagatgc gtcacccgct cggtcatgag ttgtcaccac
    23041 ttctccagga atctgccggg ggatggcaag tttgatagtt acctgtgttc cgcgccccac
    23101 ttcactttgg atggagattg atccgccgtg ggcttcgatg attttacgtg cggtgggcaa
    23161 acccaagcct gtccccccgc gctttgtggt ataaaaggca tcgaaggcat gggccagtgt
    23221 ctctgcgtcc atcccacagc cgttgtcgat caggtcaatg accacgccgt caggcgaggg
    23281 aacggtgcga atgacgagtt caccaccgtt gggcatggcc tgttgggcat tgatcaggag
    23341 attaaggagg gccgcctgaa aagcctcccg atcaagcaga accgtgggca gatcacttga
    23401 gaggaaatca atgatttcga tcccggactc tttagcacgg gcagcaaaaa aacgcaaagc
    23461 ccggcgaatc tcttcgttca aattggccgg ttctaaattt aaccgatccg cccgcgtgaa
    23521 cttcaaaaaa tcctccaaaa tagtttgcag acggtcgcac tctttctgga ccatttcaat
    23581 acgagccaaa atgcgtcgct cgcggggggt ctcaggttga gcgaagtcct ctgctagcag
    23641 ttccaggttc aggcggatcg tggaaagggg gttcttgatc tcgtgggcca gcgcgccggc
    23701 caaccgggcg agctccgtat attgatcaag gagcttttgt gaaggctcgt cgcgagttcg
    23761 cattgcttcg ccgcccattg ataaaccccc aaaatcctct tacgtgctac gacctaagtc
    23821 gtacccgacc gatccgtttt tcctgccgac gcccggactc ctgcaaacga ttatcttagc
    23881 agaaatcccc tcgattgatg agatccaagg ggaatgagca cggaatcccg aaatctcggc
    23941 gcagaacgat cgaacccaga acgatccaac tacgagcgac tggctttgcc gttgctacgc
    24001 cgtgatgggt tagcggtttc ggtcgcggtc ttgccgtcga tgggcaggcg ggaccctctt
    24061 agggggtggt ggcggaggcg gctcgacgtt accttcgcct tcccgtttct gcgcaagttc
    24121 ccggagggca cttttgcgac tgagcttgac ccgatcctga tcgtcaatgg caatcacctt
    24181 gacgggcacc gtgtcaccga ccttgcagat atcggagaca ttagcgacgt attcgtcggc
    24241 cagttcgctg atgtgacaga gtccatcttt tccgggcaag atttcaacaa aggctccgaa
    24301 gtctttgata ctggtgaccc gaccgtcata gatcttgccg acctcaactg tggccgtcac
    24361 ggcttccact tgcttgaggg ccgcctgagc atcttccaaa ctcgaggcgg caatgataac
    24421 agtcccgtcg tcttccactt cgatagtgga gcgggtcttt tcctggatgc cacggatggt
    24481 cttgccgccc ggtccgatca aggcgccgat cttgtcgggc tcgatcttcg ttcgcagtaa
    24541 acgcggggcg aagaccgaaa tatcttgccg gggccgcgaa atgaccgaga gcatcttccg
    24601 caggatttcg atgcgggcct cgcgagcctg ggccaatgtc gcccgaacaa tcgcctcatt
    24661 gatcgagcgg accttgaggt ccaattgaat gcctgtgatc ccgttctggg ttcctgccac
    24721 cttgaagtcc atgtcaccga agtggtcctc atcgccgatg atgtcggtga ggagcagcca
    24781 attatcttcc gactccttga tcaggccgat ggaaatacca gcgaccgggt tgcggatggg
    24841 cacacctgcc gccatgaggc ccaacgtcgc cccgcacacc gtggccatcg aactggaacc
    24901 gttcgactca aggatgtcgg agataatgcg gatggtgtag gggaagacat ccgggtcggg
    24961 caacaccggc tcgacgctgc gttccgccag gttgccgtga ccgatttccc ggcgactcgg
    25021 cccacggatc ggccgacatt cacccacaca aaacggcggg aagtagtaat ccagcatgaa
    25081 cttcttgctg tattcgtcga tgatgccatc gacgatctgc tcgtcgcgag gcgtgcccag
    25141 ggtcacggtg atcatggcct gggtttctcc ccgctggaaa agggccgaac catggactcg
    25201 cggcagcaca tcgaccttgc aggtgatagg ccgcaactcc ttgacccccc gaccatcagg
    25261 ccgtgttcca ctgaggatca aatcgcgaac gacccgccgt tccaggtcgt gccaagcacg
    25321 ctcgaactcg cttagaggga tggcaccttc tgccgtggga tcggggatca tctcctgcaa
    25381 ggcttgctgc ttaagtgcct cgcaagcggc cgcccgctcc tgctttcccg gtgtttgctt
    25441 ggcctgacgg aaggcctcat agtagcgggc cttcaattct tcgtagagcg gactgggcgg
    25501 gggtggctca aacggattct ttgtgggctg gaccttaccg gtcaattcct cctgaagatc
    25561 gatgacctgc ttgacgtact cataggcggc catgatggca tcgcccatga catcttccgg
    25621 cacctccttg ccgaagccct cgatcatcag gataccttcc ttcgacccgg agacaaccag
    25681 gtccaggtca ctcttctcca acatttcctg ggtggggaag gggacaatct ggccgtcgat
    25741 cagtcctacc cgcaccgaac caaccggccc gtcgaagggg atgggggaaa tccccagggc
    25801 ggccgcggaa ccgatcatgg ctaagacatc cgggtcgttc tgccggtcgg ccgccatcac
    25861 gatggcctgg acctgcactt cgtcccgaaa tccctcggga aagaggggcc gaatcggtcg
    25921 gtcaatcaaa cgcgagatca ggacttcacg cggcgaaggg cgcccttcac gcttgaggaa
    25981 gcccccgggg aactttccgg cggccgcgta ccgctcacgg taatcacaac tgagggggaa
    26041 gaaatcgacg gcttcccgcg gtgcccccgt ggtcacggca cacagaacca ccgtatcccc
    26101 gtaaccgaca agacaacacc ctgccgcctg tttgcccaac tcccctgttt caatccacag
    26161 cgtctgggcc ccaatgcgtc gttcaactcg aactttcaaa gctcaacacc tttccaacaa
    26221 gaaggccatt tcggcgcctg agaggtcgca ctttgacgag ggcgtgtccg ggcggatcca
    26281 accaccattc attaagatac gaatgcggac acaccaggac agaagcggag cccgtgccgc
    26341 acatcacttg cggaggccaa gacgttgaat aatctcaagg tatcgctgag gtgctctgcg
    26401 cttgagatag tccaacagcc gccgtcgctg acttaccatc attagcattc cgcggcggct
    26461 ggcaaagtcc ttgcgatgaa ccttcaaatg atcggtcaaa aggttgatcc gcgaggttaa
    26521 gatcgcaatc tgcacctcgg gtgaacccgt atcaccttcc ttcgtgcgaa actgttcgat
    26581 caattgacgc ttttgctctt tcgtgatggt catctgaact ccgcaagtct ttgtccgctt
    26641 ggttggttcc gcctcacatt ccctcaaaaa ggcgatttta cctccacctg gtcagccgat
    26701 tcacacatcc acaaatccca cagctatcat caaacgctag tttaacccta acagcggcct
    26761 cggaaaaggg ggtttctgtg gacaacccgc cgcagagaag cccaccgttt gctgtcactt
    26821 tcgggccgga gcgccgagat caaccttgtt gctcggccag gatgcgggcg tttttcgcct
    26881 ggagcgtcag cttctttact cgattaagat aaatctgccg ccgcacccgg ctgctgaccg
    26941 tcttggcaag ccgggcatac tttgcggcta actgttcctt gacttgcgcc aggtggagca
    27001 acatgcgtcc tttgctattg agcttttttt cgccttgtgt agctgtcatt attcgcaccc
    27061 tctgagtctt acggctaact taccgacgta aatgatgcct tgtgtgcttg ctatcttgcc
    27121 attcatacta gtgaataccc actttgtgca atccccctta gcgaggggca ggggaagagt
    27181 gaggcgacaa atgtttggaa gagaccaccc ggtggagcaa ctctgccaat tgtgatacca
    27241 tttgatcttc cgagaacctc attgtggcaa tggctttggc gcgacgggtc agcgcccctc
    27301 gctgctcctc gggcaggttt agtacctctt tgatcgcgcc ttgcaatacg gcagggtcgg
    27361 cgggaggcac catcagaccg agcggcgggt tatcgttcag aacctccgga ataccgcccg
    27421 cagagctggc gacgaccggt acacccagaa ggagggcctc gattaaggcc ccacctaagc
    27481 cttcctcacg ggacgggaag acgaacacat cggcggcggc cacgatgtca agggcatccg
    27541 accgaaaacc gggtaaaaac actcggttgc tcagccccag cgttgccatt cgtgcctcaa
    27601 gatgtgagcg ccactcaccg tctccgataa ggaccacggc cacatcggaa tggtctttgg
    27661 ctagtccggc aaacgcggtg ataaggtcgt cctgaccttt ggcgggggtt agcttcccca
    27721 catgaacaat gaaccgtgtt ccgggggagc cgttgcccga atgcacgctt tcaggatctc
    27781 cctggtcgct tcccgacggc ggagctcccc cgcaatttcg ggcgagctca gtgaggactt
    27841 gtcgcccacg aatgggattt gcctgacgga ttcggcctaa atccaccccg tcatagatga
    27901 tcgagattcg ttgggagaga atcccggctt gacgacaggc ctgggcggcc gccaaggagc
    27961 tacaaaccac accgtccagc atttggcggt acaaccagcc cgagcggaga cgaaacacgg
    28021 tacgacgggc cccaattcgg gcgacaggta agcccaaact ggccaaaagg ccgtgaatga
    28081 cggcatgggg gtcgttcata aaaaggacgt gcggccggaa ccagcgcatc gcccgccgga
    28141 gagctagcca cgccgacggt ccacgtgccc gatgaggaag ggaaaacacc gggaagccca
    28201 attggcgcaa aaggctcgcc agggcgctat cggccggggc cgccgtcaag acgtcacacg
    28261 ccctcgcggt caatccccga acaataagtc gagcttgctc ctcgcctccg ccccaggttc
    28321 tttgagtaga caacaaaaga acccgcaagc gtggtcgtgc cccggtagga tttgcataac
    28381 ggttggctaa catagatcac caacgcaaaa ccaaaaggct cgacatatgg gagccacgtt
    28441 ccaccctagg gagtcgagcc cttctcctct gccccggtga tatgtgttgc ccggcgatat
    28501 cgctcctaag aaggttaacg gaacaatcga atggcaatat tcatcaccaa cgtcaggagt
    28561 atgctcagga caatgcacgt ggctaagggg atgtggaggg accagcgctc accgcggatg
    28621 tgaatatcgc cgggtaggcg tcccaggtgc agtggcaggc cccccagcaa ccagatcagg
    28681 ccgccaatga tcaccaggaa caccccgatc agaaccagga atttacccac cgaggtgaat
    28741 tccatagcct ttctcgttcc ccgatacctt tgagcctatc ccacaacctc ccctctcccg
    28801 ttcggcgaaa gggtccgggg ttgagcgtgg cacgaatcgc taccggtttt ctcgatcgac
    28861 tccaacggtc gcttgccctc accccaaccc tctcccagag ggagagggaa ctttggcata
    28921 ggctctttac tgcgaaatga taatcactcc acacaatctt ttgtcattct atgcgcactg
    28981 acgtcaaaag gaagcgccga gaaaagggga ttccgtggta ctcgtgacgc gctcccgcga
    29041 gcttgatgcc agggagcttt tccaaccacg ggtgttttcc atgaatcatt tggataacac
    29101 cgtcaggata aggcatccaa aaaaaaacgg cacaccgtaa cactgaccgt gggttgggtc
    29161 agtgtagcgg tgcgccggtg cgggttggga ttctgctaac cattggcctg tcccaccctt
    29221 tatgaggggc acactaccag cttccggtca ggaggaggta ggtcagggca gccggatcga
    29281 cggacttggt gttggttccc ccgcgggatt tggcttgaac tttttcaaaa ttctgcaatt
    29341 ggtacagata gctgatcgag ccaaaatcac cggacaaggt tagactggtc gccgtggcca
    29401 ccagagtgtc ggcacccgga gaatcgtaca agatagctga gtcgaacccg gcccccggat
    29461 cgacctggat ctcttcgaag aatttcactc gcaggaaata gttactcccg gaaagccttg
    29521 cccattctgg ccaaccaaca aacgtgtcat cgccgtcgga gcctttgagt tgggcgacat
    29581 cccgaccacc cttcagggaa taggcgtgga cataatcgaa
//
LOCUS       DAOSUT010000033        28291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1105_length_28291_cov_9.806878, whole genome shotgun sequence.
ACCESSION   DAOSUT010000033 DAOSUT010000000
VERSION     DAOSUT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 28291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28291
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1105_length_28291_cov_9.806878"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLD05_04655"
     CDS             complement(<1..608)
                     /locus_tag="PLD05_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HOP76756.1"
                     /translation="MSTTDLRKTNVRRRLGDILVEKGYLRPEQLQAALEQQRSSGRTR
                     LLGEILLSLGYCSEEQIAECLAEEYGLPFTRLEMRLFDQKIVDLLPREYIENNSVLPL
                     FRVRDVLTVAVAEPSNLFLFQEIEHLTGLRVRPIVAPYRDIRRMIASLPNSSVFVIDD
                     ILDGSDVAEVTLVEETIEDIGNLAEIAGQSPVIRLVNYILFNA"
     gene            complement(635..976)
                     /locus_tag="PLD05_04660"
     CDS             complement(635..976)
                     /locus_tag="PLD05_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOP76757.1"
                     /translation="MQLAYEIFGDIAVVHAPEELSEDSVPALFAALEQIQVRHIVLDL
                     DRTELLDSAGLTAIVNAHEMLQQKQGSLRLVASQPINRKILHITGIEQTIPVFNTVVE
                     AAKTLHGGVSE"
     gene            complement(1028..1414)
                     /locus_tag="PLD05_04665"
     CDS             complement(1028..1414)
                     /locus_tag="PLD05_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76758.1"
                     /translation="MSKQVLLCDDEIAILKAAEFKLKKAGFAVRCADNGETAWTMVCE
                     AAPDLIISDYQMPRLDGIQLLARIRNYSVTKATPFILLTGKGFELDGEELRRTLNATE
                     LLPKPFSPRALLEKVISILGQPSCGV"
     gene            complement(1424..2941)
                     /locus_tag="PLD05_04670"
     CDS             complement(1424..2941)
                     /locus_tag="PLD05_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76759.1"
                     /translation="MFSRMVQATSNYGSRLAPAVGVGLLGLCCIFACLGPDYFRGYGQ
                     VVAGVAVAFQVVLVLRVTIQHQRAVTAIRESLVRLINLLRKSGSEQVSFTIPRGRAAR
                     EWGDKYRTLWDEIGRVHEELTLLKQQCATAEVRSRHYSLLVQRLNDLLDAFPDPVLII
                     DPSGRVVSCNRAAQSRLNIAAKSEVPANHSHDSALVDDLVEQVKKAVGANAVFDWQTA
                     GPEGRSYHYRVVVREIGSSENGNRLECGKDRAVFLQDLTPLQELRKRHAEFVSAASHE
                     MKAPLAAIRAYTEMLLDGEAGDEHTREEFISTIEAQAQRLQRLVENLLNTARIEAGIL
                     KVHKRPISVNEILQEAVRLMTAAANEKDIALLLEPSPLYLPINADRDLMLQAVINLIS
                     NAIKYTPNGGKVTVRSQLQDQTVVIEVEDTGVGMSPADVTRVFEKFYRVEAHKNMAPG
                     TGLGLPLTKHIVEDVHGGTISVRSKVNEGSVFSIALPKASAAADRARTSQELATV"
     gene            complement(3613..5910)
                     /locus_tag="PLD05_04675"
     CDS             complement(3613..5910)
                     /locus_tag="PLD05_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76760.1"
                     /translation="MSNCHGRPMKRLRRFLEIIAACGLATVAAYWLAMSDVGPGAELV
                     THTKASTSPAETALQTSPNREANSYSRPSNVEQLPSYEPGPQVYIQNKVQSEDKPNKA
                     VAGTVVNPFISNSPSTATAFPDRLVTTGYDLPARSEPDSVMVSPQETESRTIVPVNYE
                     VDPSSPVADVGSPVRLRVTSLEESPDRGRVAPITYQEANPSAVSERGLSNVDDPTLQV
                     PPGGNSEALSGLQSDAQGRSGPSQERSTQEVVPSPLPDIREMTINPPQAEITVNQAGI
                     QLELRDADIRDAVHTLAKLAKLNVVMSSSVQGKVTVSLNTLTPRDAIFAVLRVPGFVS
                     QEHEGVLYIGTEQEIQNLGYITDQIATRIYRPNYTPAKELESLIKPLLTPQVGLITVS
                     TAAENGIAPDPSNAGGDRYAGTDVLIVRDYQRVLKEVDRVVQEVDVRPAQVAIEAMIL
                     SVNLDDKDQFGVSFELLRNKGYIKLGWGLVPQSLSDIKFDNNGLKLAFLDGSIGAFLN
                     ALESIGDTNVIATPRLLVLNKHRAEILIGKELGYVSTTFTETSSSQTVDFLKVGAQLV
                     IRPFISNDGLIRMEVHPELSTGSVQITNNFTVPNKDTTQVTTNIMVPNGVTVVIGGLM
                     REDLEKTASQIPFFGNLPLIGAAFRNKMETTRKQEIIVLITPYIIDDYRMATDGATAQ
                     ASWQRRQEVAGEEMSGLNKQNASKHFLEKAKTAYLQGRCDAAKYWAEMAVHFDPSNLE
                     AIAFRDQLAGVATQNTSATLRPSNR"
     gene            complement(5942..6589)
                     /locus_tag="PLD05_04680"
     CDS             complement(5942..6589)
                     /locus_tag="PLD05_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76761.1"
                     /translation="MSKWIKKIKREFKRSPGKAAVLGGLAVVAVAFWGYRLAAAPRSV
                     SQQASVSGMGQAETETDGPVFPTQEGSIVASSEEAKVDLTPWHVLQAWRAQPFQAETA
                     SAQLARDPFVMKSPPVHQSAEESLGKSDVDDVSPATLGLRFTGVVIAGDWRAAILENR
                     LLWEGSAFKVHRNGRSYVVRICEVTPTAIKLTIDGAVFEQSLPELGLSPKGSEAP"
     gene            complement(6661..7206)
                     /gene="pilO"
                     /locus_tag="PLD05_04685"
     CDS             complement(6661..7206)
                     /gene="pilO"
                     /locus_tag="PLD05_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOP76762.1"
                     /translation="MSTPMVVLGKRNMVLVAVVAAITIALIVANMALGYRNTMLRQSI
                     GALRSRIQGQEVQLLAEKASDPQATEIEDFLTDWERRQIDVKSISEFTTQLAHEATSA
                     GLQVVRITPDKWSTTGWLSSCPFNLEFRGTFQQLLKFLRHWDYVGKAAVIHGLDIERA
                     AEAGNNLQIRIKFTVYGEKLG"
     gene            complement(7203..7976)
                     /locus_tag="PLD05_04690"
     CDS             complement(7203..7976)
                     /locus_tag="PLD05_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76763.1"
                     /translation="MSIQFDFLPKEYHQTNLRRRNVPWLVSMVLLVVLVGSGGLVVQM
                     GTRRQLWTTYQQLTQQCMANEVKLAQYQHQKAKKRDALRKARLLQPILLGYPRSWLLE
                     AIVSSTPEDVVLDQVRLTLKEEQQVTESQEAPVDFLRSGSGGSADKATEELIRAQEKL
                     SRAWIEVVIDGRSVSTTAVQDFCSQLSHFPVIEEINLQTVEGMSNEKIGQGSTHFVLW
                     GRCRHLPPPAAFVRPDQVPAMTTGPLANVGRHGSKANLP"
     gene            complement(8048..9115)
                     /gene="pilM"
                     /locus_tag="PLD05_04695"
     CDS             complement(8048..9115)
                     /gene="pilM"
                     /locus_tag="PLD05_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOP76764.1"
                     /translation="MTGFSVFRRLSPIGMDIGHRRVRAVQLNGDRSAVVRAMEMEITA
                     SEADDSDRSALIAKIIREVLREGRFQCRNVVFAMSHPDLSVQNIRINDAPEKPLTESV
                     LEEVAARANINQENVEIRCIDAGLVQQGEIQRREVVLLTCRKTAISERIQIANQAGIR
                     LVGLDAEPAALARAVLQQYRRQSDRTQTMLLIRMGAEHTLVMILRGDQPLFAKYLNFG
                     GHDLDQALVRQFQIPIESALVLRQGYGERRRDHRDPEVVRTVEECLEPLWNSWLEEIA
                     RCARYYQVTFRGQPIERVILAGSEATPELREKLESFLDLPTEVLDPFRAIRCHETVDA
                     ARDWDLAIGLALYSPATPCLV"
     gene            complement(9170..9787)
                     /locus_tag="PLD05_04700"
     CDS             complement(9170..9787)
                     /locus_tag="PLD05_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP76765.1"
                     /translation="MKTIQEHRRVGEAICPKDGRFAFTLMEVLIVIMLMAIVVALVIP
                     SAAPQTEQRLRAAAHLVAAEVELARDLAITYNTKYKLFFDIKANRIVLTHAGENTALD
                     QLPSELVSNTASDPTERVLDLSSAPGLWGQVRIAACASFDPMLKPQDEIIIGPTGTPI
                     DGKSFLIWLRDGEAQWKRYITIVIDQSTGRVSIGSMNGMAPPFGE"
     gene            complement(9753..10058)
                     /locus_tag="PLD05_04705"
     CDS             complement(9753..10058)
                     /locus_tag="PLD05_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76766.1"
                     /translation="MSKKSFFLACTLTVIIIMCMLVLGERVVVPAPSVAEQPFANSVE
                     QRAQMISELRAIRQLLSDQNTLLREQNRLLSEQLNVLKKISSGESKRENHPGAQASR"
     gene            complement(10089..11660)
                     /locus_tag="PLD05_04710"
     CDS             complement(10089..11660)
                     /locus_tag="PLD05_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76767.1"
                     /translation="MNYLCCHLSRTMSRWRFRLGFHPDLGTVRNPRSRRRGFILLLVI
                     LTIISAMALTYVVASTTRLSMRKVRNAQLQKAAIAATETGLSVGLANMCSSKWGGIGS
                     QISGACGLNQAFEVTYEHGDPTLDAASPEWTDCPYRVTIRAIGKAWHPEEPEYPAIYR
                     RSWVVQLVPRAVAAEPSDWSRILNYTFFQTAVTASSVEIPVRIVGKCRFQSTLNLAVK
                     YPTYNPRVRYMSDLYEMQANGYGDWRTFTGRIYFPVSSQPLDTIDLLVNRLRVFINDA
                     WVNLPSSDLALPQQPTSYRLYPGGKSYSIPQLGSALENTTLSPDPDNNPCGILYAPGN
                     IAIRSNVTIKGSLLCNGDVRIEGTNIVISGQPIWGIQADVPVELPVIVCKNLHFKAGS
                     ACQINGMVGLFGELQAPKAPTLTIVEITGRVFATAIKIDSLSPWDSFDWDKALTQFIK
                     EKDSLTGENRYFPFWLRQFGLDVTPRLIIQPPQEKRYFHWKKTADPVYVPANSDTTSI
                     DPNPGLRWELIYELD"
     gene            complement(11669..12616)
                     /locus_tag="PLD05_04715"
     CDS             complement(11669..12616)
                     /locus_tag="PLD05_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76768.1"
                     /translation="MFLASSFDSRITRSLRWVRHSDRRDGQAEKVGLRRKPLASDHDP
                     HRRGISHQTPKSYGVGFTLVEMLAAVALTGLIVVGIVAFAQLAEDSFATGQDSSETIQ
                     IARTCFQQIESMLNAAWGNELFPGALVVPRLVSGTQFPETLVIWCPVTGQPRNPDGLP
                     CVDELVVLSPDSSRPHELRKYRVSNNSATVPSPSNVSAWQSLIHGIQSQSPAQSQLLT
                     DRLRTFTVNNVTLGAIRFRVETVPSDSEWADSSVTWSTLSWPQGLYGPNRGVRRVSVH
                     TEIQLRKKTGSLTPQPEASCSFWHSHAYLYTVRKEKRLQ"
     gene            complement(12594..13238)
                     /locus_tag="PLD05_04720"
     CDS             complement(12594..13238)
                     /locus_tag="PLD05_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOP76769.1"
                     /translation="MYLSQAIVNLRTCQELRRHDHLLVGVAFTLIETIIALGLMAVVV
                     GAVTYSLVSSLQVAEENQELAIAEGLAQDLMNEILNRRYCELGTSPYDPYLRPGSVEK
                     YPGTRELFDDIDDYNGWVEEPPQDRWGIPLGEEDGVGGRRPYPARAASLSGFRREVIV
                     SYVSSQDFSKTLPFGSVSDYRKIEVIVSRRYPNNVVRQLVKLTRVVGHVPSEQF"
     gene            13631..13996
                     /locus_tag="PLD05_04725"
     CDS             13631..13996
                     /locus_tag="PLD05_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76770.1"
                     /translation="MKMRLIFLAIVLGLGMTSLVLSGERPRYVRGVGLAGLASPSCGV
                     CADCCPTCPAPQAPCPVRGPMGWGFPHHGWRLGLGCIGRDPGQNIQPGPPTAAITYPY
                     YTLRGPRDFLQRDLSPIGP"
     gene            complement(14063..15424)
                     /gene="mgtE"
                     /locus_tag="PLD05_04730"
     CDS             complement(14063..15424)
                     /gene="mgtE"
                     /locus_tag="PLD05_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOP76771.1"
                     /translation="MSNEAHTIAKRIIEALDRRDHEELVRLLQKVPEGELPQRLAQLT
                     EADQRRLLEAIPAEVASSLLQELPESQVTRLLEELAPSQAAAILDHLPSDEQADLLGA
                     LPDEEASKLLEIMNPEEASDARRLLQFAPDTAGGLMITEYLAYTETCTVEDIVEDLRI
                     NAARYRSYDVQYFYTVDAEGRLTGVLRVRDLLLSEPDTQIATIAVRQPVSVATDTSLD
                     KLEHLFAHYQFYGLPVVDRDGRLVGLVRRYDVEEAIAARANRNFLKVVGISGGEELRS
                     MPLRDRIKGRTSWLTVNLILDLIAASVIPFFEETISAVIALVFFLPIISDMGGNTGIQ
                     SIGVTLRELAAGLITPRDAWRTFWRELPVAVINGLVVGFLLAGVALVWRGNPYLGILI
                     GSALTLNTVIAACLGAVLPLGLRRIGIDPAVASGPILTTIADLTGFFMVLGGAAVILP
                     YLT"
     gene            complement(15457..16635)
                     /locus_tag="PLD05_04735"
     CDS             complement(15457..16635)
                     /locus_tag="PLD05_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOP76772.1"
                     /translation="MIAQESSVSLSTWDEFRSLMPICGRYVYFDHAAVAPLPGPTRQA
                     IESWLLEATEDGDTRWPEWAQRLEAFRRDIAEFIGALPEEIALVRNTSHGISIVAEGF
                     PWQAGDNVVIPAQEFPANQYPWLALESFGVEVRRVPPKHGVIDLKALEEACDARTRIL
                     SVSWVDYLSGFRIAPAELAALAHRYGAYFFLDAIQGLGVFPIDVHQVEIDFLAADGHK
                     WLLGPEGAGVFYCRAPLWEMLRPTSIGWNSVEHPFEFDRIDPNWKKSAARFEGGSQNI
                     IGFLGLHASLSLLRRYGPAAISQRVLDLTDAICEVLRSLGAILLSRRDEGVASGIVTF
                     SIPDLDVIRLREALLRRRIVLSVRQKALRVSPHAYNNEHDVAAFGEALREAVDECRAS
                     "
     gene            complement(16679..18481)
                     /gene="pepF"
                     /locus_tag="PLD05_04740"
     CDS             complement(16679..18481)
                     /gene="pepF"
                     /locus_tag="PLD05_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOP76773.1"
                     /translation="MAKAQGLPPRSARKPEDCWDLSSLFPSDEAWEAALHAWEEAIPG
                     YERFRGKLSQGANVIAECLEFDYRMDREGERLGTYAFLKTAEDTTNPHYQRMRGRFLS
                     IATRANQAASFIRPELLAIPRRKMEQFLKDAALRSYEVPLRRILRWRPHTLGASEERI
                     LAMQTQMAETPSQIFHQLNDGDLRFGYVKDEKGRTRELTHGNFLSFLQSPDRAVRGAA
                     FETFYEKYAEHQNTLAATLSGSVQKDVYYARVRNFPSAREAALFPDNIPTQVYDQLIE
                     TVRRYLPSLHRYYDLRRRKLRLRQLCVYDTYVPLVPRLRLRTSWDQAVALVLKALAPL
                     GDEYVSILEKGFQSRWCDRYENRGKQSGAFSAGTYDGAPYILMNYQPDVFDHVFTLAH
                     EAGHSMHSYYSAHHQPYHLYQYTIFVAEVASTFNEQLLHRYLMGQAKSDEERAYYINH
                     EIDEIRGTIFRQTMFAEFEKMIHEHVEKGDALTVETFRTMYRSLLNDYFGPDVKVPDL
                     LELECFRIPHFYNAFYVYKYATGLSAAIALADRVLQGGKNELDAYLAFLSAGCSKDPL
                     DLLRDAGVDMERPDPVAAAMERFDTLVDELEKIL"
     assembly_gap    18806..18905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18906..19850
                     /locus_tag="PLD05_04745"
     CDS             <18906..19850
                     /locus_tag="PLD05_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76774.1"
                     /translation="EATDLDAIAIFTEAPNHVKHTVAAMNHGKHVVCAVPAAMTLEEC
                     QTLLDTVKKTGLTFMMAETSYWQQHTISARKFVEQGEFGEIFAMYSMYRHAGLESLWF
                     EDGKPTWRHGFPPMHYPTHCTAHCIGVTGERLVAVSCTGWGDDSPLLKGNPYNNPFWN
                     ETAEFVTDKGHSFRVEVWWAGAHVGCERAEWYGTKMSFICENRLGTQATLIFAGEQKE
                     LDSGGFVRAASRVEPYEIPQWWATEMLPEPLRHDSGHEGSHTFITHEFIDALICERRP
                     AVDVYEAIAYTAPGIVAHQSALRDGERLTIPDFGKAPA"
     gene            complement(19923..21413)
                     /locus_tag="PLD05_04750"
     CDS             complement(19923..21413)
                     /locus_tag="PLD05_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP76775.1"
                     /translation="MIARIVSVTIIVCALLTTGQGRAVGQALSGKPSVRNVILLVSDD
                     HRYDFLGFMTQCPSFLETPNLDRMAREGAHCVNAFVATSLCSPSRASILSGLYMYHHQ
                     VVDNQRPVPPGTVFFPQILQQAGFQTAFIGKWHMGHDDDSPQPGFDYWASFRGQGEYF
                     DPILNINGNRRQIKGYNTDVLTDLAIDWLKRGRDPSKPFFLYVGFKAVHYPFTPAPRH
                     QGRYHGKRIDYPETMANTEQNYRSQPKWVRERRFSIHGIDHMETGQFDKDPVPNFDEL
                     YWNYCETVFSLDEDIGRILDAVAAQGLDQNTVIIYMSDNGFALGEHGFYDKRDAFEVS
                     IRVPLLVYAPGLIRPGTKIEAMVQNIDIAPTVLELCGVALPATKPFDGRSFLPLLLGQ
                     QVPWRDHILYEYYWEWNFPATPTMFAIRTDRYKYVYYHGVWDYDSFHDLQTDPHERYN
                     LIDVPAYQELIQKLKQQLFDELESSGALMMPIRPPAGERLDQRKLE"
     gene            complement(21484..>22541)
                     /locus_tag="PLD05_04755"
     CDS             complement(21484..>22541)
                     /locus_tag="PLD05_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOP76776.1"
                     /translation="FFWGHRNATVISCELEDKKTMNRLPLLGCVMCLCLQTATFESVA
                     WQIPVSGPDPTVIAVDSNGALSYYAFVTGRGIPVLHSTDLKSWQPIGRVFEQAVPDWA
                     RREVPGATGIWAPDISYHEGRYYLYYAVSTFGSQRSVIGLAVNRTLDRESPDYRWVDC
                     GKVIESSPNGCDYNAIDPALFVDNDGRWYLFFGSFWSGIKAIELIPGEGKPKPDAKIV
                     SIAGRPHHPTHAIEAPFVVFREGFYYLFASWDRCCDGADSDYKVVVGRAKQVLGPYVD
                     AQGKPMLDGGGTLILAGNDRWRGPGHNCVLHTETGDWIIHHTYDMQNLDKHRILQVRP
                     LSWTEDGWPRVGQPLSN"
     assembly_gap    22542..22641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22642..23704)
                     /gene="wecB"
                     /locus_tag="PLD05_04760"
     CDS             complement(<22642..23704)
                     /gene="wecB"
                     /locus_tag="PLD05_04760"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOP76777.1"
                     /translation="MAPILRALRSYPDIRPILIHTGQHYDPQLSDVFFQDLEMPPPDI
                     HLNVGSGSHAQQIARILEKMEEVLADGAPDGGRFSRIIVVGDVNSTLAAALAAAKLEI
                     PVAHVEAGLRSFDRSMPEEINRLVTDTLADMLFVSEPSGVENLRQEGHSNDQIHLVGN
                     VMIDTLFALLPRAQGLDTLARLGLRPKEYAVVTLHRPANVDQRETLGGLLKVLADVAL
                     QLPIVFPIHPRTQKRIESFGLQHLLDNHKSSQGLNVTPPLGYLEFLSLTSQAKVVITD
                     SGGLQEESTALGIPCLTVRPNTERPITVTEGTSTLIGNDPDKLRTCLEEVLNGRYKTG
                     RCPDLWDGHAAERIAAILAH"
     gene            complement(24481..26250)
                     /locus_tag="PLD05_04765"
     CDS             complement(24481..26250)
                     /locus_tag="PLD05_04765"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOP76778.1"
                     /translation="MSLRRLFSTVRYYRLGQIFYRLIGRAKALRKHQTIRNLCARSPL
                     EVRAGLPMIADPVADGPRLIVDGNAFVLRLLNSEYSSEGVIDWMAIEKQIPTRLGRFH
                     LHYHEYFLPKSREDVLPASASLIWNCIAQWIDTFSGSSGHVSLDAWHPYVISRRIPVW
                     VKLFSLCPPPGDLARLILTSLTAQARFLRRNLEFDLGGNHLMQNLRGLAVAGAFFYGS
                     EAEEWLATVVRRLEQQCREQILEHGEHFERSPMYHVDVLLAMADIRDALAAARKTYPT
                     FLDQYIEKMATFLGKILHPDGQIPLFGDSTLDLTPQPRQVFTRLQMGFPSLCPPQPQS
                     YAVGDYWIYREQENFLIFDGGPVGPDYLPAHAHADLLTVEASWAGTRLFVDGGVYDYE
                     DSPERAYCRSTAAHNTAEINGQNQCDVWSRFRMGRRGWPGKLQTGLTGRFHWARCTHN
                     AYRHLNVPSVGRWIVCTEGGPWCIVDSCKGSPSVTMASRLRLAPGWIPSHETPNRLTL
                     ERGSVRLLVENILGCGPIKCDIGNYYPEFQKAVQIPVLYYETTNRGSCQLAWLVSGEP
                     FGPFVFKPLSREGALAGNSVSKS"
     gene            complement(26625..27932)
                     /locus_tag="PLD05_04770"
     CDS             complement(26625..27932)
                     /locus_tag="PLD05_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOP76779.1"
                     /translation="MHILFLTHYFPPEVNAPASRTYEHAVRWVRAGHRVTIITCNPNC
                     PTGILFPGYKNRLRPQRENMDGMEVVRVWTYLAPNAGTVRRIVNYLSFMVSAVLVGLW
                     LEKPDVIVATSPQFFCGWAGVLLSRLKRLPFVLEIRDIWPESIETVGAIRFRPVLRFL
                     QFLERVMYRSADHIVAVGEGYRQKILEKVNIPQRISVITNGVDLERFRPQPLDKELRR
                     KWNGEGKFVCAYVGTIGMAHGLEVVLDAAERLRDQGRNDIVFWLVGEGARKAYLENEA
                     RQRGLKDRVIFTGRLPKEEMPRVLASADACLVHLRKTELFETVIPSKIFEMMAMGKPI
                     IMGVRGEALRIVEESGAGVAMEPESADSLLMAIEHIRSADHFGNSRTSSVRQYLEDHF
                     SRDALATKMLSIISQLVSPGQQHRNDERPTEEQLQDSMTPVSH"
     gene            complement(27968..>28291)
                     /locus_tag="PLD05_04775"
     CDS             complement(27968..>28291)
                     /locus_tag="PLD05_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76780.1"
                     /translation="GALVRLMELPEARGQVFNVGNREEVSIKELAERVICLTGSRSQI
                     RYIPFAEAYGADFEDMQRRVPDLTKIERMIGYRPRYSLDDIIRDIVEYYANKLGLSTR
                     LGTLR"
BASE COUNT         6689 a         7810 c         7148 g         6444 t
ORIGIN      
        1 gcgttgaaca aaatatagtt cacgagccga atgaccggtg actggccggc gatttcggcg
       61 aggtttccga tatcctcgat cgtctcctcc accaatgtga cctctgcgac gtcgcttcca
      121 tcgaggatat cgtcgatcac aaagacactc gagttgggta gcgacgcaat catccgtcga
      181 atatctcggt aaggagccac aatcggtcta acgcgcagcc ccgtgaggtg ttcaatctcc
      241 tgaaagagaa acaggttgga cggttctgcc acggcgactg tcaacacatc gcgaacccga
      301 aagagcggta gtacggagtt gttttcaata tattcccggg gtaaaagatc gacaatcttt
      361 tgatcaaaga gacgcatctc aagccgagtg aacggcaatc cgtactcttc cgcaaggcat
      421 tctgcaatct gttcttcgga gcagtatccc agggaaagca aaatttcacc aagtagcctg
      481 gtgcggccac ttgaacgttg ctgttctaga gctgcctgaa gttgctcggg acgaagatac
      541 cccttttcga cgaggatatc gccaaggcgc cgtcgcacat tcgtttttcg caaatccgtc
      601 gtggacatgt ttgattccat tacctgacgc gtggttattc cgatacccca ccgtgcaacg
      661 tcttggctgc ttcaacaact gtgttgaata caggaatggt ctgttcaatt cccgtgatat
      721 gaaggatttt gcggttaatt ggttggctgg cgaccaggcg gagactgcct tgcttttgtt
      781 gaagcatctc gtgggcattg acaatggccg tcagaccggc actatccagc aactcggtcc
      841 tgtccaagtc aagcacaatg tgcctgacct gaatctgctc caatgctgca aaaagggccg
      901 gcacactgtc ttcgcttagc tcctctggag catgaacgac cgcaatgtca ccaaaaatct
      961 catatgccaa ttgcatcgtg gttccttaaa cggtgttcat tctcttaggc tcaaccttgt
     1021 gctcagctta caccccacac gagggttggc ccaaaattga gatcaccttt tcgagtaaag
     1081 cccgggggga aaaaggcttt ggcaacagct ctgttgcatt gagggttcgt ctcaattctt
     1141 caccatccaa ttcgaacccc tttcctgtca gcaggataaa gggagtggcc ttggtcactg
     1201 aataattccg aatacgcgcc aggagttgaa tcccgtccag tcgcggcatt tgatagtcac
     1261 tgataatcaa atcaggagcc gcttcgcaca ccatggtcca tgcagtttcc ccgttgtccg
     1321 cacagcggac ggcgaaacca gcctttttca acttgaactc ggctgccttc aaaatggcaa
     1381 tttcgtcatc acaaagaagc acctgcttgc tcatggaatc tgctcacacc gttgcaagtt
     1441 cctgagacgt ccgggcccgg tccgccgcag cagaggcctt tggtagtgca atgctaaaca
     1501 ccgaaccctc gttcacttta ctgcggacag aaatggtccc accatgaaca tcttctacaa
     1561 tgtgtttcgt cagcggcagg ccaagacctg tcccgggcgc catattctta tgagcctcga
     1621 cacgataaaa cttctcgaag acacgcgtta catcggcagg tgacatgccg acgcccgtgt
     1681 cctccacttc aatgacgacg gtctggtcct ggagttgact ccgcacggta acttttccgc
     1741 cattgggcgt gtatttgatt gcgttcgaaa tcaaattgat gacggcttgc agcatcaggt
     1801 cacgatcggc attgatgggc aggtagagcg ggctaggctc aagcaatagt gctatatcct
     1861 tttcatttgc cgcggcggtc attagacgga ccgcttcttg aaggatctca tttaccgaaa
     1921 taggccgttt gtggaccttg agaatccccg cctcgattcg ggctgtgtta agcaagtttt
     1981 caactagcct ttgcaacctt tgggcttgtg cttcgatagt ggagatgaac tcctcacggg
     2041 tgtgttcatc tccagcctcg ccgtccagga gcatttcggt gtaggcccga atagccgcta
     2101 atggtgcctt catttcgtgg ctagcggcgg acacgaactc tgcgtgccgt tttcgcagct
     2161 cctgaagtgg tgttaaatcc tgcaagaaaa ccgcgcgatc ttttccacac tcaagacgat
     2221 ttccgttttc cgaggaaccg atctcccgga ctaccacccg ataatggtaa gagcggcctt
     2281 ctggacctgc cgtctgccag tcaaacacag cattggcacc cacggccttt tttacctgtt
     2341 ccaccagatc gtcgaccaag gcgctatcgt ggctgtggtt ggcgggcacc tcagatttgg
     2401 cagcaatgtt cagtcgactt tgggcagcac ggttacagga gacaacacga cctgacgggt
     2461 caatgatcag tacggggtcg ggaaacgcgt cgagtaaatc attgagcctt tggacgagga
     2521 ggctgtaatg tctcgagcgg acctcagcgg tggcgcactg ttgtttaaga agggtgagtt
     2581 cctcatgcac tcggccaatc tcatcccata acgttcggta tttatcaccc cattccctgg
     2641 ccgccctgcc ccgcggaatc gtgaaagata cctgttcact tccggattta cgcaataagt
     2701 ttatcagccg taccaatgac tcccgaatcg cagtaacggc ccgttgatgt tggatcgtca
     2761 ccctgaggac aagaacgacc tgaaacgcga cagcgacccc agcgacaacc tggccatatc
     2821 cacggaagta atccggacca aggcaagcga agatgcaaca taatccaagt aggccgactc
     2881 ccacggccgg agcgagccgc gacccgtagt tcgatgttgc ttgaaccatt cgactaaaca
     2941 tgggcaactc tcggttgaga tgctgccatt aaccggggct aattccaaca acgaagtaag
     3001 cccacccagg agggggcgtc tgggatgagg attcacattc ctcattagaa ggctagtacg
     3061 aaactagagg ggtgtccact gcaaagtcgt cgtcggggga cgatgagttt aaagtcctcc
     3121 gtgagtcttt ccagactagg tagacccaac tgagactgcc cttctttttg ttacaaccaa
     3181 ttccttcggt gtcaccagca gttcggatac actcgataca tccggcacgc tccacctact
     3241 actgacctcc aaaggagcta tagcgactcc gagcttcgct taacttggcc gccacttcgt
     3301 tctcgatggt ctgccttcga tagcgtcgca tatccgagag agactgccga tgaacatcag
     3361 gtgtatctat tggagcacat tgagcctcag catgagttgg ccatggatgc ggctggactg
     3421 agcgacatcc tagggaacaa agaagcccca aaaccgagag actaaaaccg atcaccagca
     3481 aacgacgctt gcccgccaac atttgttgca tcagatcaac ttagagcatg acgcttgtta
     3541 tactaccggg cactttctcg gaaatgtcaa gaaactcttc acaataacag acaaaaacct
     3601 gcggcagaat tatcagcggt ttgacggtcg taaagtcgcg ctagtatttt gcgttgccac
     3661 accagctagc tgatcgcgaa aggcaatagc ttccaggttc gacggatcaa agtgcacagc
     3721 catttcggcc cagtatttcg cagcgtcgca acgtccctgg agatacgccg tttttgcttt
     3781 ctccaggaaa tgctttgagg cattttgctt atttagtcca ctcatctcct ctcccgccac
     3841 ctcttggcgt cgttgccatg acgcttgggc cgtggcaccg tctgtagcca ttcgataatc
     3901 atcgataatg tagggcgtga tcaagacaat gatttcttgc tttctcgttg tttccatctt
     3961 gttccggaac gccgcaccga tgagaggtag atttccaaaa aaggggattt gcgaggccgt
     4021 cttttccaag tcttctcgca tcaaaccacc gatgacgacc gtcacgccgt tggggaccat
     4081 gatgttcgtc gtgacctgcg tcgtgtcctt attaggaacc gtaaagttgt tcgtaatctg
     4141 cacggagccc gtggagagtt ccgggtgaac ctccatccga atcaggccat cgttggaaat
     4201 gaacggccga atcacaagct gggctcccac cttcaaaaaa tccacggtct gcgaggaact
     4261 ggtctcggta aacgttgtgc tcacgtaccc aagttctttt cctattagaa tttccgctct
     4321 gtgtttgttg agaacgagaa gccggggcgt agcgatgacg ttggtatcgc cgatcgactc
     4381 taaggcgttg aggaaggcac cgatgctccc gtcgagaaag gccaacttaa gaccattatt
     4441 gtcaaacttg atatcgctta gactttgggg gacaagtccc caaccgagct tgatgtatcc
     4501 tttatttcgc aatagctcaa agctcacccc aaactggtcc ttgtcgtcaa gattgacgct
     4561 gagaatcatg gcctcgatgg cgacctgcgc cggccggaca tcaacctcct gaacaacgcg
     4621 gtcaacctcc ttgagaaccc gctgataatc tcggacaatg agcacgtccg ttcccgcgta
     4681 tctgtcaccc ccagcattgc ttgggtcggg cgcaatcccg ttctctgccg ctgtggaaac
     4741 ggtaatcaga cctacttggg gtgtgagaag aggctttatc agcgactcta actcctttgc
     4801 cggcgtgtag ttcgggcgat aaatgcgggt ggctatctgg tcggtgatgt agcccaggtt
     4861 ttgaatctct tgttccgtcc cgatataaag gacaccctca tgctcttgtg atacgaaccc
     4921 cgggacccta agaacggcaa aaatggcgtc gcgcggggtc agagtgttga gagagacggt
     4981 gaccttacct tggacactgc tcgacatgac gacattgagc ttggccagct tggcaagggt
     5041 gtggacggca tcccggatgt cggcgtcacg tagttcgagt tggattcccg cctgattgac
     5101 ggtaatctcc gcttgaggcg gattgatcgt catctctctt atatcgggca atgggctggg
     5161 gacaacttcc tgggttgagc gctcttgaga aggtccgcta cgcccctgcg cgtcagattg
     5221 caatccggag agagcttcgc tatttccgcc cggcgggacc tgaagcgtcg ggtcgtccac
     5281 gttggataag cccctctcgg atactgcact tgggttcgct tcttggtacg taattggtgc
     5341 aacgcggcct cgatccggtg actcttcaag cgatgtcaca cgaagccgaa cgggactgcc
     5401 gacatcggct accggcgaac tgggatccac ctcgtaattg accggcacga tggttcgcga
     5461 ttctgtctct tgcggggaaa ccataacgga atccggctca gaccgtgccg gcaagtcgta
     5521 acctgttgtg actaaccgat ctggaaaagc tgtagccgtt gagggggaat tgcttataaa
     5581 aggattgact acagtaccgg cgacggcttt gtttggcttg tcttcggact gcaccttatt
     5641 ctgaatataa acttggggac caggctcata cgagggtaat tgctccacat tggatggccg
     5701 agagtatgaa tttgcctcac gattagggga agtttgtaag gccgtctctg cgggagatgt
     5761 cgatgccttc gtatgggtca ccagctcagc accgggccca acatccgaca tggcaagcca
     5821 ataggctgcc actgtagcca ggccacaagc ggcgataatc tccaaaaacc tgcgtagccg
     5881 tttcatcggt cgtccgtgac agttactcat ccatgtcttt agctatgagg ttgccgctta
     5941 tctaaggtgc ctcgctgccc ttggggctca gcccaagctc gggcagcgat tgttcgaaaa
     6001 cagccccgtc gatggtaagt ttgatagccg tcggcgtaac ttcacaaatc cggacaacat
     6061 aagaccgtcc atttcggtgg accttaaatg cggagccttc ccaaagcaag cggttttcga
     6121 ggatcgctgc ccgccagtca cccgcgataa ccacgccggt aaagcgaagt cccagcgtcg
     6181 ccggagaaac gtcgtcaaca tcagatttgc ccaacgactc ttctgctgac tggtgtacag
     6241 gtgggctctt catgacaaaa gggtcccgcg cgagctgcgc cgaagcagtc tcggcttgga
     6301 aaggctgagc acgccatgcc tggagtacat gccaaggcgt taagtccact tttgcttcct
     6361 cgctactcgc cactatggat ccttcttgcg tgggaaagac cgggccatcg gtttccgttt
     6421 ccgcctgtcc cattccgctc accgaagcct gttgggaaac cgagcgagga gccgcggcca
     6481 accgataccc ccaaaaagcc acggcaacca ccgccaaacc tcccaaaaca gcggccttgc
     6541 ctggacttcg cttaaactct cgttttatct tttttatcca tttgctcatg cgtcgcgtca
     6601 cctttgggtg ctgggcgcca catcattcat cggaaatcct tcggattttc cctaaactaa
     6661 ttaacccaat ttttcgccat aaacggtaaa cttgatccgg atctgcaaat tgtttcccgc
     6721 ttcggcggct cgctcgatgt caagtccatg aatgaccgcg gccttcccca cgtagtccca
     6781 atgcctgaga aatttcagca gctgttggaa agtgccccta aattccagat tgaacgggca
     6841 agaactaagc cacccagtgg ttgaccattt atctggcgtg attctgacga cttgcaaccc
     6901 cgccgaagtg gcttcgtgcg ccagttgcgt ggtgaactct gagatggact tgacgtcaat
     6961 ctgacggcgc tcccagtccg ttaggaagtc ttcaatctcc gtggcctggg ggtcggaggc
     7021 tttctccgcc aacaattgga cctcctgccc ctggatacgg ctacgcaatg caccgatcga
     7081 ctgtcgtaac atcgtgttcc ggtaaccgag ggccatgttg gcgacaataa gcgcgattgt
     7141 gattgccgcc accacggcaa ccagcaccat atttcttttt ccgagaacta ccattggggt
     7201 actcatggta agttcgcctt agatccatgc cggcctacgt ttgccagcgg ccccgtggtc
     7261 atcgctggaa cctgatcggg ccgaacaaaa gcggcaggcg gcgggagatg ccgacatcgg
     7321 ccccacagaa cgaagtgggt tgatccttgc cctatctttt cgtttgacat cccttccacc
     7381 gtttgcaagt tgatttcttc gatcacggga aaatgagaga gttggctgca aaaatcttgc
     7441 accgccgtgg tcgacacact tcgaccatca atcacaacct cgatccaagc ccgcgacagt
     7501 ttttcttgag cgcggataag ctcttcagta gccttatccg cagaaccgcc tgaaccggag
     7561 cgtaagaagt caaccggcgc ctcttgagac tcggtgactt gctgctcctc cttaagagtc
     7621 aatcttactt ggtcaaggac aacgtcttcc ggtgtacttg agacgattgc ttccagtagc
     7681 cacgaccttg ggtaaccgag caagataggc tgcaaaagcc gggccttccg aagggcgtct
     7741 ctctttttgg ctttttggtg ttggtattga gccaacttca cctcgtttgc catacactgt
     7801 tgagtcaatt gctggtatgt agtccacaat tgacgacgcg ttcccatttg aacgactagc
     7861 cccccgctgc caacgagcac taccaaaaga accattgaca ccagccaggg gacgttccgc
     7921 cgcctgagat tggtttgatg gtattctttc ggtaagaaat caaattggat cgacatcgtt
     7981 caaacaactt aactcattct aagttggccc cgctcttacg aaccgctttt cctttcggct
     8041 gtatcctcta aaccaagcac ggagtcgcgg gagagtaaag cgccaagcca atggccaggt
     8101 cccaatcccg tgccgcatcg accgtttcgt gacatcgaat cgcacgaaac ggatcgagca
     8161 cctccgtggg caagtcaaga aaactttcaa gcttttctcg cagttccggt gtggcctctg
     8221 agccagctag gatcacacgc tcaataggct ggccacggaa cgtcacctgg taataccggg
     8281 cacaccttgc aatctcctca agccaagagt tccacaatgg ctcgagacac tcctcaaccg
     8341 ttcgtactac ctctggatcg cgatggtctc gtcgtcgttc cccatatcct tggcgaagga
     8401 ccaaagccga ctcgattgga atttggaatt gcctgaccaa ggcttgatct agatcgtgcc
     8461 ccccaaagtt cagatacttt gcaaacagtg gttggtcacc tctcaaaatc attacgagcg
     8521 tgtgttcggc ccccattcgg atgaggagca tcgtttgggt tcgatcgctc tgccgccgat
     8581 attgctgaag tacagcccgc gctaaggccg ccggctccgc atcgaggccc accaatctga
     8641 tccccgcttg gttcgctatc tgaattctct cgctgatagc cgtcttccga cacgtcagaa
     8701 gaaccacttc acgccgttgg atctcgcctt gttgcacgag acccgcatcg atacaacgta
     8761 tctccacgtt ctcctggttg atgttggcgc gcgcagcgac ttcctccaaa acactctcgg
     8821 tgaggggttt ttcaggtgca tcattaatgc gaatgttttg caccgataag tccgggtggc
     8881 tcattgcgaa gacgacattg cggcattgga accggccttc acgcagaact tcccgaatga
     8941 tcttggctat cagtgccgag cgatcagaat catctgcttc cgaagcggta atctccatct
     9001 ccatcgctcg gaccacagcc gaccggtcac cgttcaattg gacggcgcga acccgacggt
     9061 ggccaatatc cataccgatc ggtgatagtc ttcggaagac agaaaaaccg gtcatcgctt
     9121 tcacattact cttctttgct gtcgttgatt actcccggca agctgggttt cactctccaa
     9181 agggaggggc catcccgttc atagaaccga tagatactcg gcctgtactt tggtcaatga
     9241 caatggtgat atatcgtttc cactgggctt ctccgtccct caaccaaatc agaaacgatt
     9301 tgccatcaat gggggtacct gtgggtccaa tgataatctc atcttgaggt ttcaacatcg
     9361 gatcaaagct cgcgcaggcc gcgatacgga cttgtcccca aagcccgggt gcactcgaaa
     9421 gatcaagaac tctctcggtc ggatccgatg ctgtattgct gacgagttcg ctcggtaatt
     9481 gatctaaggc cgtattctct ccggcatggg taagaacaat acgattcgcc ttaatatcaa
     9541 agaagagttt gtacttggtg ttataggtaa tcgctaagtc ccgcgccagt tccacctcgg
     9601 ctgccaccag atgggccgcc gcgcgaagtc gctgctcggt ttgaggggct gcgcttggta
     9661 ttaccaatgc cacgacgatt gccatgagca taatcacaat gagaacttcc atcagcgtaa
     9721 aggcgaatcg accatccttc gggcagatgg cctcaccgac tcgcctgtgc tcctggatgg
     9781 ttttcacgtt ttgactctcc ggacgaaatc ttcttaagaa cgttcaattg ttcggagagc
     9841 aaccggtttt gctctcggag cagggtattc tgatcggaca acaattgccg gatcgcccgc
     9901 agttcgctaa tcatctgcgc ccgttgctcc acactattgg caaagggctg ctcggccaca
     9961 cttggtgctg gaacaacgac cctctctccc aacactagca tgcacatgat gattatgact
    10021 gtaagcgtac aagccagaaa aaagcttttc ttcgacatga tcgttacact ccctctaata
    10081 tgaatttcct aatccagctc gtagatgagc tcccatctca gtccgggatt cgggtcgata
    10141 gatgtcgtat cggagttggc ggggacatag accgggtcgg cggtcttctt ccagtggaaa
    10201 tatcgcttct cctgaggtgg ctgaataatt aaacggggcg tcacatcaag tccaaactgt
    10261 cgcaaccaga acggaaaata cctattctcg cccgtgagcg agtctttttc ctttatgaat
    10321 tgggtcaatg ctttatccca atcaaaagag tcccatggcg acaatgaatc gattttgatc
    10381 gctgtagcaa agactcggcc tgtgatctca acgatcgtca aggttggcgc cttgggggcc
    10441 tgcaactcac caaaaaggcc aaccattccg ttgatctgac atgccgaccc ggccttaaaa
    10501 tggagattct tgcatacgat aacaggtaac tccacgggga cgtcggcttg gattccccag
    10561 atcggttgcc cactgatcac aatgtttgtt ccctcgatcc ggacgtcccc attacagagc
    10621 agactccctt tgatcgtgac gttggaacgt atcgcgatgt ttcctggcgc atacagaata
    10681 ccacacggat tattgtccgg gtcaggcgat agcgtcgtgt tttccaatgc cgaccccagt
    10741 tgcggaatcg aatagctttt gcccccagga tacaaccggt acgatgtggg ctgctgcggt
    10801 aaggcaaggt cgctactggg caggttgacc cacgcgtcat ttataaaaac tcgaagccga
    10861 ttgacaagaa ggtcaatggt gtcaagaggt tgggacgaga cagggaaata gatacggccg
    10921 gtaaacgttc gccagtctcc atatccattg gcctgcatct cgtaaagatc gctcatgtac
    10981 ctcacccgag gattgtacgt cggatacttg actgccaggt tcagagtact ttgaaaccga
    11041 cacttgccca caattcgcac aggaatctcc acagatgaag ccgtgaccgc tgtctggaaa
    11101 aaggtgtaat taaggatcct cgaccaatcg gaaggctccg ccgctacagc ccgaggcaca
    11161 agttgaacaa cccagctcct tctgtaaatg gcggggtatt caggttcttc tgggtgccat
    11221 gccttgccga ttgcgcgaat ggtcacccga tacggacaat cagtccattc cggtgacgcg
    11281 gcatcgagcg tcgggtcacc atgttcgtag gttacctcaa aagcttgatt taagccgcag
    11341 gccccgctta tttggctgcc aatgcccccc cacttggaag aacacatgtt ggccaatcca
    11401 accgacaagc ccgtctctgt ggcagcgatg gcagctttct gcaactgggc gttgcgaacc
    11461 tttcgcatgc tcagccgcgt cgttgaagca acgacgtacg tcaaagccat tgccgaaatg
    11521 atcgtcagaa tcacgagtag gagaatgaac ccccgacgcc gcgaacgggg gttacgcacc
    11581 gtgcccaagt caggatgaaa cccaagtcgg aatcgccagc gactcatggt ccgtgaaaga
    11641 tggcaacaaa ggtaattcat gataacagct actggagtct cttctctttc cgcactgtgt
    11701 acaaatacgc atgggaatgc cagaagctgc aactggcttc cggttgcggt gtgagcgagc
    11761 ccgtcttctt gcggagttgt atctctgtgt gcaccgaaac ccgccggact ccgcgatttg
    11821 gaccgtataa cccctgtggc caggaaagtg tgctccatgt aacgctggag tcggcccatt
    11881 cactatcact ggggactgtt tccactcgga aacggatggc cccaagcgtg acattgttaa
    11941 ccgtgaatgt tcgtagccga tcagtcagaa gctgactttg tgcgggcgat tgactttgta
    12001 ttccgtgaat gagcgattgc cacgctgaaa catttgacgg actaggtact gtagcactgt
    12061 tgttcgagac gcgatatttt ctcaattcat ggggtcgaga actatccgga ctgagcacca
    12121 caagttcatc gacacacggc aaaccatctg gatttcgcgg ctggccggta actgggcacc
    12181 agatgacgag tgtctctgga aattgagttc ccgaaaccaa cctcggcacc acaagtgcac
    12241 ccgggaatag ctcattaccc caagcagcgt tcagcatgga ttcgatctgc tgaaaacatg
    12301 ttcgcgcaat ctggattgtc tctgaactat cttggccagt tgcaaacgag tcttcggcca
    12361 gttgggcaaa cgccacgatg ccaacaacga ttagaccagt caaggcgacg gcagcgagca
    12421 tttcgacaag cgtgaacccc accccatagg atttgggcgt ctgatgcgag attccgcgac
    12481 gatgtgggtc atgatccgac gccaacggct ttcgtcgcag accaaccttc tcggcctggc
    12541 cgtctcgtcg atcggagtga cgtacccaac gtaacgatcg ggtaatgcgg gaatcaaaac
    12601 tgctcgctag gaacatggcc gacaactcgt gtaagcttga ccagttggcg cacgacgttg
    12661 ttgggatacc gacggctgac gatgacttcg atctttcgat aatccgaaac cgaaccaaaa
    12721 ggtagggttt tcgagaaatc ttgggaacta acgtaactga caatgacctc acgacggaag
    12781 ccgctgaggc tcgcggctct agcaggatat gggcgccttc cgcccacccc gtcttcctct
    12841 ccgaggggaa ttccccagcg gtcttgcggt ggctcttcca cccagccgtt ataatcgtca
    12901 atgtcgtcaa aaagctcgcg cgttcccggg tacttttcga cactgcccgg acggaggtag
    12961 ggatcatacg gtgacgttcc caattcacag taacggcggt tgagtatttc gttcatcaag
    13021 tcttgggcta acccctcggc gatggccaac tcttgattct cttcagccac ttgcaaagaa
    13081 cttaccaacg aatacgtcac cgcacctacc acaacggcca tcaagccgag agctatgatg
    13141 gtttcaatca acgtaaaggc tacccccacc aacaggtggt cgtgtcggcg taattcctgg
    13201 catgtccgca aattgacgat cgcttgactc agatacattt ttttctggcc ataaaacggg
    13261 tcacgtttac gatgtcacat aaatataggc cgcgatgcaa tcttcggggc gacctatctt
    13321 gccggagaac ttgggcatcc cccaaggggc ctattaccca taaaatgcat cttgttgagc
    13381 ccggttgccg ctggtaggag gacgtcgagg gtttcgccaa aaccgccata tcttggtaat
    13441 attgtggaaa tgcgaaactt aacccgatac gcctttgacg aaacgttgga ttgtgccagc
    13501 gcttctatcc gatgatagta ataagggttt tgggggccgt ttttggacgc aacgcaggcg
    13561 aatgacccgg cggtcacaat tcccgatgtg aatcgagcga aacagaacaa gaaagaacga
    13621 ggaccaacct atgaagatga gactaatttt cctggccatt gtcttgggcc ttgggatgac
    13681 atctctagtt ctatcaggcg aaaggccacg atatgttcgt ggcgtggggc ttgccggact
    13741 ggctagccca agctgtggcg tatgtgcaga ttgctgtcct acctgcccag ccccccaggc
    13801 gccttgccca gtgcggggac cgatgggttg gggattcccg catcacggtt ggcggctggg
    13861 gctcggatgt atcggtcgtg atcctggcca gaatatccag cctggtccac cgacggccgc
    13921 cattacttac ccatattaca ccctccgtgg gccgcgggac ttcctgcaac gtgacctaag
    13981 tccaatcggt ccgtgattgc tcgacgcgaa cttttttcgg ctctgtcgta aaccgcagaa
    14041 ccggtggtgc ccgccaccgg ttttatgtta ggtacggtaa gataacggcc gcgccaccaa
    14101 ggaccatgaa aaagcctgtc aggtccgcaa tggtggttag gatggggcct gaggcgaccg
    14161 ccggatcgat tccaatacgg cgcaagccaa gtggcaaaac ggcccccaga catgcggcaa
    14221 taaccgtgtt taacgtcagg gccgagccga tgaggattcc caagtaagga ttccctcgcc
    14281 ataccaacgc cacgccggcg aggaggaatc cgacgaccaa gccgttaatc acagcgaccg
    14341 gaagctctcg ccagaatgtt cgccaagcat cccgaggggt gatcaaacct gctgccagct
    14401 cgcggagcgt gacgccaatg ctctggatcc ccgtgtttcc gcccatatcc gagataatgg
    14461 gtagaaagaa aactaaggcg ataacggcac tgattgtctc ttcgaaaaac gggatcacgc
    14521 ttgctgcgat aagatccaaa atgaggttaa ctgtcaacca tgaggtgcgg cccttgatac
    14581 ggtcgcggag gggcatggac cgcaattctt ccccaccaga tatccccacc accttgagaa
    14641 agttccgatt cgccctggcg gcaatcgctt cctccacgtc gtaacgccta accaatccca
    14701 ccagtcggcc gtctcggtcc acaaccggga gcccgtaaaa ctggtaatgg gcaaagagat
    14761 gctcgagctt atccaaagaa gtatccgtgg cgacactcac cggctggcgg acggcgatag
    14821 tcgcaatctg tgtatcaggt tcagaaagca aaagatctcg cacccgcagg acgccggtaa
    14881 gcctaccctc agcatcgacc gtatagaaat actgaacgtc gtaactacga taacgagcag
    14941 cattgatcct taggtcttcg acaatatctt ccacggtaca agtttcggta tatgccaggt
    15001 actccgtgat catgagaccg ccggcggtat ccggggcaaa ttgaagaagt cgcctggcgt
    15061 cagaggcttc ttctggattc ataatttcta acagtttgct ggcctcttcg tccggcagcg
    15121 cccccagcaa gtcggcctgt tcatcactcg gaagatgatc gagtatggcc gccgcctgcg
    15181 atggcgctag ctcctcaagc agccgtgtta cttgactctc tggcagttcc tgaagaaggc
    15241 tggacgcaac ctcggccgga atcgcttcca gcaaccgcct ttgatcagcc tcggtcaact
    15301 gggcgagcct ttgcggtagt tcaccctccg gaaccttctg aagaaggcga accaactcct
    15361 cgtgatcgcg ccggtcaagc gcttcaataa ttctttttgc gatagtgtga gcttcgttcg
    15421 acataaaaga ttcacgtcct taaaaatctt tctcgatcaa ctggcccggc attcatccac
    15481 cgcctcgcgg agtgcctcgc caaatgctgc aacgtcatgc tcattgttgt aggcatgtgg
    15541 acttacacgg agggctttct gacggacact cagaacaata cgccgtcgca gcagagcctc
    15601 ccggagacga ataacatcga gatcaggaat tgagaatgtc acgattcccg aggcaacgcc
    15661 ttcatcacgc cggctgagaa gaatggcacc gagacttcgc aacacctcgc agatggcgtc
    15721 tgtgaggtcg aggacccgtt ggctgattgc cgctgggcca taacgtcgaa gtagcgacag
    15781 agaagcgtgc aatcctaaaa atccaatgat gttttgcgat ccaccctcaa atcgggcagc
    15841 agattttttc cagtttgggt cgattctgtc gaactcaaaa ggatgttcga cgctgttcca
    15901 tccaatgctt gtaggacgaa gcatttccca cagcggagcg cgacaataaa agacccctgc
    15961 cccctccggc ccgagcagcc acttatgccc atctgccgca aggaagtcga tctccacctg
    16021 gtggacatct atcgggaaca cccctaaacc ctgaatcgca tccaggaaaa aataagcccc
    16081 atatcggtgg gccaatgcgg ctagctccgc cggggcaatg cgaaaacccg aaagataatc
    16141 cacccagctc acagacaaaa tgcgcgtccg ggcatcacag gcctcctcga gggccttcaa
    16201 gtcgataaca ccatgcttgg gcgggacacg tcgcacttca accccgaaac tttccagggc
    16261 aagccacgga tattggttgg caggaaactc ttgggccggg atgactacat tgtcgcccgc
    16321 ttgccaggga aaaccttcag ccacgattga tatcccatgg ctcgtattgc ggaccagggc
    16381 aatctcttcg ggcagtgcac cgatgaactc ggctatgtca cggcgaaagg cctcgaggcg
    16441 ctgggcccat tctggccaac gggtgtcacc atcttctgtt gcctccagaa gccacgattc
    16501 gatggcttgt cgagtcggac ctggcagggg tgccacagcc gcgtgatcaa aatacacata
    16561 ccggccacag atgggcatca aagaacgaaa ctcatcccaa gttgaaagcg aaacagacga
    16621 ctcttgcgct atcatgtcga cgctcgtttc tcgcttcaaa aatgccgatc ccagcctgtc
    16681 acagaatctt ttcaagttcg tcaaccagcg tatcgaaccg ctccatggcg gccgccaccg
    16741 ggtcggggcg ttccatatcc acccctgcgt cccgcaaaag atcgagagga tccttagaac
    16801 aacccgcgga aagaaacgcg aggtaggcgt ccaattcatt ctttccacct tgaaggacgc
    16861 gatcggccaa cgcaatggca gcggacaggc ctgtggcgta cttatagaca taaaatgcgt
    16921 tgtaaaaatg cgggatgcgg aagcattcca gttcaagaag gtcaggcact ttcacatctg
    16981 gcccaaaata atcattgaga agcgagcgat acatcgtccg gaaagtttcg accgtcagcg
    17041 catcaccttt ttcaacgtgc tcatgaatca tcttttcaaa ctcggcaaac attgtttgac
    17101 gaaagatggt cccgcggatt tcgtcgatct catggttgat gtaataggcc cgctcctcat
    17161 ccgatttggc ctgccccatc agatagcgat gcagcaactg ctcattaaat gtgctggcaa
    17221 cttcagcaac aaatatcgtg tactggtaaa ggtgatatgg ctgatgatgg gccgaatagt
    17281 agctatgcat cgaatgcccg gcttcgtgag ctaaggtaaa gacatgatca aacacatcgg
    17341 gttgatagtt catcaggata tagggtgccc catcgtacgt cccagcacta aatgccccac
    17401 tctgcttccc acggttctcg tagcgatcgc accaccgtga ctggaacccc ttttctagaa
    17461 tactcacata ctcatcacca aggggcgcaa gggcctttag tacaagggca accgcttggt
    17521 cccagcttgt ccgtaatctt agcctaggga ccaggggaac atatgtgtca taaacacata
    17581 actgacgcag acgtagcttc cgccggcgga ggtcgtagta acgatgcaac gatggcaggt
    17641 accgacggac ggtctctatt aactggtcat acacctgtgt ggggatatta tcaggaaaca
    17701 atgcggcctc acgggccgag ggaaaattac gaacccttgc gtaatagaca tctttctgga
    17761 cggaccccga tagcgtcgcc gcaagcgtgt tttggtgctc ggcgtatttt tcgtaaaatg
    17821 tctcaaacgc tgctcctcgg acagctcgat ccggcgactg aaggaaactt aggaaatttc
    17881 cgtgcgtgag ttcgcgagtc cggcccttct cgtccttaac gtagccaaat cgcaaatcac
    17941 catcattgag ttgatggaaa atctgagagg gggtctcagc catttgtgtt tgcatcgcca
    18001 gaattcgttc ttctgatgcc cccagcgtgt gaggtctcca gcggagaatt cgtcgcaacg
    18061 gcacttcgta tgaacgaagc gcggcatcct tgagaaattg ctccattttc ctccgaggga
    18121 tggccaacag ctctggccga ataaaactgg cagcctggtt cgcccgggtt gcgatgctca
    18181 agaaccgacc gcgcatcctc tgatagtgcg gattagtcgt gtcctcggcg gtcttgagga
    18241 aggcgtaagt ccccagccgt tcgccttcac gatccatccg ataatcgaat tccaagcatt
    18301 cggcgatgac attcgcacct tggctcagct ttccacgaaa tctctcatac ccgggaattg
    18361 cttcctccca tgcatggagc gctgcttccc aggcctcatc actcggaaaa agacttgaca
    18421 aatcccagca atcttccggt tttcgagcac ttcgcggagg taaaccttga gctttcgcca
    18481 tggaacatcc tctcctgagt ttgtatcgca ccttgttctt gatctcgtgc tgccgatctc
    18541 ccataattgg ggctcgtacg gcgtcctttt gcggattatg gtttgtttgt gccgtagcgc
    18601 caacgcgggg tcacttcgga ccgagaaagc cgcttttcgg tccagtatta cgaagaagcc
    18661 gaaccgcgac gggacattaa gccatagcaa cacattgttc gtagagggtc tttttatgac
    18721 cagaaagaac tcaagacgta cgtcaattgg gttggtgcac aaagatgagc aatcgaggcg
    18781 gtcattcctt aaaatgacgg ccgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnngaagc cacggacctc gacgcaattg ccatttttac cgaggccccc aatcatgtga
    18961 agcatactgt cgctgccatg aaccacggca aacacgtggt ttgtgccgtg ccagccgcga
    19021 tgaccctgga agagtgtcag accctgctcg ataccgtcaa gaaaaccggc ctgaccttca
    19081 tgatggccga aacaagctac tggcagcagc atacgatttc ggcacgtaaa ttcgtcgagc
    19141 agggggagtt tggtgaaatc tttgccatgt attcaatgta tcgccatgcg ggacttgaat
    19201 cgctttggtt tgaagatggc aagccgacat ggcgacatgg ttttcctccc atgcactatc
    19261 ctacccattg cacggcgcat tgtattggcg tgacgggcga acgccttgtg gccgtctcct
    19321 gcacgggctg gggtgatgat tcaccactcc ttaagggtaa tccctacaat aaccccttct
    19381 ggaatgaaac ggccgagttc gtgaccgaca agggacactc ttttcgtgtc gaggtctggt
    19441 gggcaggggc ccatgtgggg tgcgaacggg ccgaatggta tggcacaaag atgagcttca
    19501 tctgcgaaaa ccgcctggga acccaggcga ccctcatctt tgccggcgag cagaaggaac
    19561 tcgatagcgg cggattcgtc cgtgctgcat cgcgcgttga gccttacgag attccccaat
    19621 ggtgggcaac cgagatgctt cccgaacctt tgcggcacga ttcagggcac gagggatctc
    19681 acacgttcat cacacatgaa ttcattgatg ctctgatttg cgaacggcga cccgcagtgg
    19741 atgtgtacga agctatcgcc tacacagccc cggggattgt ggcacatcaa tcggccttgc
    19801 gcgacggcga acggctcacg attccagact ttggaaaggc cccggcttga cggacccgcc
    19861 tcagcagttg gccgacacgg gtttttgccg aacagttcgt agctacaccc gcggtacaag
    19921 attcactcga gcttccgttg gtcgagacgt tccccagccg ggggccttat cggcatcatg
    19981 agcgccccgg aggattccaa ctcgtcaaag agttgctgtt tgagtttctg gatcaattct
    20041 tgataggctg gcacatcgat gaggttgtat cgctcgtggg ggtccgtctg aagatcatga
    20101 aaactgtcat aatcccaaac gccgtggtaa tagacgtact tgtaacggtc ggtgcggatt
    20161 gcgaacatcg tgggcgtagc cggaaaattc cactcccaat aatactcgta gagaatgtgg
    20221 tcccgccacg gtacctgctg acctaacagc agcggcaaga acgaccgacc gtcgaagggc
    20281 tttgtggcgg gcagagccac cccacaaagt tctaggactg taggagcaat gtcgatattt
    20341 tgaaccatcg cctcaatctt cgtcccggga cggataagac caggtgcata gaccaaaagc
    20401 ggaacgcgga tcgagacctc aaaggcatcc cgcttgtcgt agaagccgtg ttctcctagg
    20461 gcaaagccat tatcggacat gtaaatgata acggtatttt gatccaagcc ttgagcggcg
    20521 acggcatcaa gaattcggcc gatgtcttcg tccagagaaa acaccgtctc acagtaattc
    20581 caatagagtt cgtcaaaatt cggcacaggg tccttgtcaa attgaccggt ctccatgtga
    20641 tcgataccgt gaatgctaaa tcgtcgttct cggacccatt tgggctgcga gcggtaattc
    20701 tgctcagtgt tggccatcgt ttcgggatag tcgatcctct tcccgtgata tctcccctgg
    20761 tgccgaggcg ccggtgtgaa ggggtaatgg actgccttga agcccacgta aaggaagaac
    20821 ggcttggaag ggtcacgtcc tcgcttcaac cagtcgatgg ccaagtcggt cagcacgtcg
    20881 gtgttatacc ccttgatttg tcttcgattt ccattgatgt tcagaatcgg atcaaaatat
    20941 tccccttgcc cgcgaaaact ggcccagtag tcaaacccag gttggggcga atcgtcatca
    21001 tgccccatgt gccactttcc aatgaaggcc gtctgaaatc cggcttgctg aaggatctga
    21061 ggaaagaaca cggtccccgg aggcacaggc cgttggttgt cgacaacctg atgatggtac
    21121 atatacaggc ccgacaaaat cgaagcccgg ctcggagagc ataatgatgt tgcaacaaat
    21181 gcgttaacac aatgtgcacc ttcacgggcc atccgatcta ggttaggcgt ctccaaaaaa
    21241 gacgggcact gagtcatgaa tcccaaaaag tcgtagcgat gatcgtcgct caccaaaagg
    21301 atcacatttc gcacagatgg ctttcctgat agcgcctggc caacggctcg tccctgcccc
    21361 gttgtcaata aagcgcaaac aatgatggtg acgctgacaa ttctcgctat catgggaacg
    21421 actccacgaa aacgcgttgc acaaatacca cgggcctaag gcagcccgcc taacctgcct
    21481 aatttagttt gaaagaggct gaccgacacg cggccaaccg tcttccgtcc acgaaagagg
    21541 ccgaacttgc aagattcgat gtttatccag gttttgcatg tcgtaggtat ggtgaatgat
    21601 ccagtcgcct gtctcggtgt ggaggacgca attgtgcccc gggccacgcc aacggtcgtt
    21661 tccagccaag atcagtgttc cacctccgtc aagcatcggt tttccctggg catccacata
    21721 aggccccaac acctgcttcg cccgtcctac aaccaccttg tagtcgctat cggcaccatc
    21781 acaacagcga tcccaggacg cgaaaaggta atagaatcct tcacggaaca ccacaaacgg
    21841 agcttcaatc gcgtgggtag ggtggtgcgg ccgtccagcg atcgaaacaa tctttgcgtc
    21901 tggcttgggt ttgccctcgc ctgggatcaa ttcaatggct ttgatccccg accaaaacga
    21961 gccgaaaaac agataccatc taccgtcatt gtcaacaaac aaggcggggt cgatggcatt
    22021 atagtcgcat ccgttcgggg acgactcgat gaccttacca caatcaaccc accgatagtc
    22081 agggctttct cgatcgaggg ttctgttgac agcaaggccg atcacagatc gttggcttcc
    22141 aaatgttgac acggcgtaat acagatagta acgcccctca tgatagctta tgtcgggggc
    22201 ccaaattccc gtagcaccgg ggacctctcg ccgagcccag tccgggactg cttgctcaaa
    22261 aacacgccca attggttgcc aagacttcaa gtcggtcgag tgaagaaccg ggattccgcg
    22321 ccccgtgaca aaggcgtaat aggacaaagc cccattggaa tccacagcga tcaccgtggg
    22381 atccggtcca gacaccggaa tctgccaggc cacggactca aaggtcgccg tctgaagaca
    22441 taggcacatt acgcagccta ataggggcaa acgattcatt gtctttttat cctcaagctc
    22501 acaggaaata accgtcgcat tacggtgacc ccagaaaaaa gnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn ntgtgggcga gaatagccgc aatccgctcc gccgcatggc
    22681 cgtcccacaa gtctggacag cgaccggtct tgtagcgacc gttcaaaacc tcctccaaac
    22741 acgtccgaag tttatcagga tcgtttccta tcagtgtgct agtcccttcc gtgacggtga
    22801 tggggcgctc ggtatttggc ctcaccgtta agcagggaat gccgagcgct gtgctttcct
    22861 cttgaagccc gcccgagtcg gtaatcacca ccttggcttg agaggtcagc gagagaaact
    22921 cgaggtagcc gagtggcggc gtgacgttca atccttggga ggacttgtga ttatcaagca
    22981 aatgttgaag gccgaaagac tcaatgcgct tttgcgttcg gggatgaata ggaaagacta
    23041 tcggcagctg taaagccaca tcagccagga cctttagaag cccgcccagc gtttcacgtt
    23101 gatcgacatt tgccgggcgg tgcaacgtca cgaccgcata ctctttggga cgcaaaccca
    23161 accgggcgag cgtgtcgagc ccctgggcgc gaggcaagag cgcgaaaagg gtgtcgatca
    23221 tcacgtttcc cacgagatgg atctgatcat tggaatgccc ttcctggcga agattctcaa
    23281 caccagaagg ctcagaaacg aacaacatgt ctgccaacgt gtcggtgaca agacggttaa
    23341 tttcttcagg catgctgcgg tcaaagcttc gcagccccgc ctcgacatgg gcaacgggga
    23401 tttccagctt ggccgctgcc aaggccgcgg caagagtgga attgacatca cccaccacga
    23461 tgatacggct aaagcggccc ccgtcaggcg cgccgtcagc aaggacctct tccatctttt
    23521 ccaagatgcg ggcaatctgc tgcgcatgtg acccggaacc aacgtttaga tgaatatccg
    23581 gcggcggcat ctctaaatct tggaaaaaca catcactgag ctgcggatca tagtgctgac
    23641 ctgtgtggat aagaattggc cgaatgtccg gataactccg cagtgcccgg agaatcgggg
    23701 ccatcttcat aaaattgggc ctagcgccga caacacaagc tagctttcgc accataggaa
    23761 tttggccctg tcacgccttt tattaaaaac tctcaacgca cgtttacctc gctcgaaaac
    23821 tcgcgaatac tccttttcgc acggaaagcg tcgtctttag cacacctcgg aaaaatgcga
    23881 tctatgtacc gtcttctatt gctatcttag ctgacagagt gttcaaatgc acaaataacg
    23941 tttgtatcaa gtgtatcaat tattcttagt acaaacttgc cactgaaatt gaatcggccc
    24001 gcgctatgag ggacaaaccg aaaaaaccta aaacacttga aagccgcaga ccggtgcaat
    24061 caaaaacgtg tcttcttatt ttgagaattg aatttagagc acagtcaatg cgaaacggat
    24121 cattgtttcg ctagtggcta gccggtctgt ggaatacggg tgaattgtta aaacaagcct
    24181 tacctggcca ttgacgcgaa caggtacccg ccggttatgg ctattgttct cgagatggaa
    24241 acgaggtgtt tctcgtttcg ctgaggtcca tcgaaaacag gaaatccaac gcgcggtatg
    24301 atctaacgcg ttggttgttc ttagaatctc ccctcgcccg ttcggcggga gaggggtcgg
    24361 gggtgaaggc ggctcgaatc gctgccggtt ttctcgatcg atttcgacat tcgcttaccc
    24421 tcaccccatc cctcgcccga agggagaggg acactttttg cgatagactg taagtccgca
    24481 tcaagatttg cttacgctat tccctgcgag agccccctcc cggctgagag gtttaaacac
    24541 aaagggcccg aaaggctcgc cagaaactaa ccacgcaagt tggcatgaac ccctgttggt
    24601 cgtctcatag tacaaaacgg gaatctggac cgctttctga aactcgggat aatagtttcc
    24661 gatgtcacat ttgatgggcc cgcaacccag aatgttttca accaacaagc gaaccgatcc
    24721 gcgctccaaa gtaagtctat tcggtgtctc atgcgatgga atccatcctg gtgccaatcg
    24781 tagtcggctg gccattgtga ctgaaggtga tcctttacac gaatcaacga tgcaccaggg
    24841 tcccccttca gtgcagacaa tccatcggcc gacgctcggt acgttcaagt gtcggtaggc
    24901 attatgagtg caccgcgccc agtgaaatct tcccgttagc cctgtctgca gtttccctgg
    24961 ccagccccgg cgccccattc gaaaccgcga ccacacatca cactggttct gcccattgat
    25021 ttccgcagta ttgtgcgccg ccgtgctgcg gcagtaggct ctttccggag aatcctcgta
    25081 gtcataaacc ccgccgtcca cgaatagcct tgttcctgcc caggaggcct ccactgtcag
    25141 caagtcggcg tgggcgtgag cgggcaagta atccgggccc accggccccc cgtcgaagat
    25201 caaaaaattt tcttgttcac gatagatcca atagtcacca acagcatagg actgtggctg
    25261 tggcgggcaa agactaggaa agcccatttg cagtcgggta aacacctgtc tcggttgtgg
    25321 agtcaagtca agggtgctat caccgaaaag aggaatctgg ccatcggggt gcaagatctt
    25381 tcctaggaaa gttgccatct tttcaatata ctggtcgaga aaagtggggt acgtcttacg
    25441 agccgcagcc agggcatcac gaatatcagc catcgccagc agaacatcca catggtacat
    25501 cggcgatcgt tcaaaatgct cgccatgttc caaaatctgt tcccggcact gctgttcaag
    25561 tcgtctcacc accgtcgcta gccattcctc agcctccgat ccgtaaaaaa aggcgccggc
    25621 tacggctagt ccccgcaagt tttgcattaa atgatttcca cctaggtcaa actcaaggtt
    25681 tcttcgaaga aaacgggcct gagccgttaa ggacgtcaaa atcagtcttg ccaaatcccc
    25741 tgggggtggg cacagagaaa agagttttac ccacaccgga atccgtcgcg aaatcacgta
    25801 tgggtgccaa gcgtcaagcg aaacgtggcc cgatgatccg ctgaaagtat cgatccattg
    25861 ggcaatgcag ttccagatta gcgaggccga cgcaggtaat acatcctctc gagacttggg
    25921 caggaaatac tcgtggtaat ggaggtgaaa tcgccccaga cgcgtcggaa tctgcttctc
    25981 gatcgccatc caatcgatga caccctccga ggagtattcg gagtttaaaa gacgcaaaac
    26041 gaaagcgttg ccgtcaacaa taagccgcgg cccatcggct actgggtcgg caatcatggg
    26101 aaggcccgcg cgcacttcca gcggcgatcg ggcacaaagg ttacggatag tctggtgttt
    26161 cctgagcgcc ttggcccgcc ctatcaaccg ataaaagatc tgccccaacc gataataacg
    26221 aacggtagaa aataggcgac gaagagacac ggcttcattc gctaaacggc atagttgaag
    26281 tcacaaagac gcttttgcct tcgctcaaag aaagcccaca tttctaggat caaaagttcg
    26341 cataaaaatt gaatgcaggt tgccagttgg aaatcttttg gaaaaatcga gtcggtcccc
    26401 aacgaccctg gattcgtttg atcagcgaga aaacccaact gcgtgactga tatgaggcct
    26461 taaggccgac gtcaagttcg acatactttc gatgagttga aagaaaacga atgaccttga
    26521 aaaccgatcg ccaacccgtg tcgtcgaagg cgagcagccc gccttcccaa agtatcgccc
    26581 tgatagtcgt cgattgtgca atgaactcgc ctttgctcat ccgatcaatg actaaccgga
    26641 gtcattgagt cttgtaattg ctcctcagtt ggccgctcgt catttctatg ttgttgtccc
    26701 ggtgacacca gttgagagat tatagatagc atttttgtgg ccaacgcgtc cctggagaaa
    26761 tgatcctcta aatactgccg aacagatgag gtcctagaat taccaaagtg gtcggcgctc
    26821 cgaatgtgct caattgccat tagcaatgag tcagccgatt ctggctccat tgccacacca
    26881 gcgccggact cctccacaat gcggagagcc tcgccgcgca cccccatgat gatcggcttt
    26941 cccatggcca tcatctcaaa aatcttgctc ggaataacag tttcaaatag ctctgttttt
    27001 cgaagatgca ccagacacgc atctgcgctg gccagcacac gaggcatttc ctcttttgga
    27061 agccgacctg tgaaaatcac acgatccttc agcccacgct gccgcgcctc gttttcaagg
    27121 tatgctttgc gggccccttc cccgaccaac cagaagacaa tgtcattccg gccttgatcc
    27181 cgcaagcgtt cggcggcgtc taatacgacc tccagaccgt gcgccattcc aatggtacct
    27241 acatacgcac acacaaactt gccctcgccg ttccatttcc ggcggagttc cttatcgagg
    27301 ggctgcggtc gaaatcgttc caaatccacc ccatttgtga tgaccgaaat acgctgcgga
    27361 atgttgacct tctccaatat cttctgccgg tatccctcgc caacggccac gatatggtct
    27421 gcggagcgat acatcactcg ttcaaggaat tgtaggaaac gtaacacagg ccgaaaccga
    27481 atggcaccta ccgtttctat ggactcgggc cagatatcgc gaatctccaa gacgaatgga
    27541 agacgtttaa gccgagacaa caggacaccg gcccaaccgc agaaaaactg tgggctggtc
    27601 gccacgatca cgtccggctt ttccagccat aaaccgacca gtactgcgct taccataaag
    27661 gaaagatagt tgacaatccg tcgcaccgtt cccgcattgg gagctaagta cgtccagaca
    27721 cggacaactt ccattccgtc catgttttct cgctgtggtc ggagtctatt tttgtaacct
    27781 ggaaagagaa tccctgtggg gcaattagga ttacacgtga tgatagtaac cctgtgacca
    27841 gcccgcaccc agcggacagc atgttcgtac gtccgacttg ctggcgcgtt cacctccgga
    27901 gggaaatagt gagtcaggaa aagtatgtgc atcttatcac ctgcccactg ctcgctagct
    27961 tggatcgtta gcgaagcgtc cctaatcgag tacttaaacc aagtttattc gcgtagtatt
    28021 ctacaatgtc tctaataatg tcgtccaaag aatatcttgg ccgataacca atcattcttt
    28081 cgatcttcgt caggtctggg acacggcgct gcatgtcctc aaaatcagct ccataagcct
    28141 ccgcaaaggg gatataacga atttgtgagc gacttccagt caggcagata actcgctcag
    28201 ctagctcctt gatagaaact tcctctcggt tgcctacatt gaaaacctga ccccttgcct
    28261 cgggcaattc catgagccta accagggcac c
//
LOCUS       DAOSUT010000034        28073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1113_length_28073_cov_9.796738, whole genome shotgun sequence.
ACCESSION   DAOSUT010000034 DAOSUT010000000
VERSION     DAOSUT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 28073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28073
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_28073_cov_9.796738"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLD05_04780"
     CDS             complement(<1..187)
                     /locus_tag="PLD05_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76781.1"
                     /translation="MNRIWLALLGTVGMAVGFHALAETTYTYRDLLMQMVDPRAAAVL
                     PEDGEFCKQWSSWDRASR"
     gene            complement(411..683)
                     /locus_tag="PLD05_04785"
     CDS             complement(411..683)
                     /locus_tag="PLD05_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76782.1"
                     /translation="MGFLARFDDPATVEAICRKIRISQEKHGSRWLAIVGHADCGGNP
                     VSPEEHHRQIRAAVEFLRQRFPTMTVIGLWLGEDWIVQEIAGEEGG"
     gene            complement(658..801)
                     /locus_tag="PLD05_04790"
     CDS             complement(658..801)
                     /locus_tag="PLD05_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76783.1"
                     /translation="MSFATVVNCMDGRTQGPVISYLKERFGVDYVDNSTEAGPDGLFS
                     PVR"
     gene            complement(846..4025)
                     /locus_tag="PLD05_04795"
     CDS             complement(846..4025)
                     /locus_tag="PLD05_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOP76784.1"
                     /translation="MCDTRNVPQSRRGYDCGCQASGLPRRTFLQLATTAAALAAWKGP
                     IVAGPFEPADTADHFVPVDKKLSPEWIAQLFARGESTWYSGEDLKNIGMPIGGICAGQ
                     LYLAGDGRLFHWDIFNRHNFSGYGANNYQQMPPPDYPVEQGFAISVQTDGKEIRRTLD
                     QAGFPEVRFCGEYPIGYVDYHDPNFPVQVRLEAFSPFIPLNEEDSALPATILAFSIKN
                     PGPETKTVTLMGWLENAVACHTGISFWGERLTHAKRYSQSVALVHSVQPSPPPEEPRP
                     VKVLADFEGENYGNWTVEGEAFGQGPARGTLPNQQTVSGFLGKGLVNTYLGGDRPHGR
                     LVSPPFVIDRRYISFLIGGGGHAGKTCMNLLVDGQVVRTATGTNNERLQWQNWDVREF
                     EGKTAQIEIVDRESGGWGHINVDQIELRDTPRPAPEGPLEKQPDFGQMALVFFLPPAS
                     DEVGKYELLAVADGGGPLDVETLWHQFNPLQGETTLGKQRLHEKLRGALGCRVSIPPG
                     KTAQLTFAVLWHFPNRPERGNYYAVKFPTLDDLARYLEANLPRLTEQTKLWYATWYGA
                     TLPHWLLDRLFSTVSNLATGTCQWWANGRFWAWEGVGCCHGTCAHVWNYEHALARLFP
                     RLERSVREMQDFNPEAGFDEQTGAVRFRGEGWKLWAGDSQGGTILKAYREHQCSRDDG
                     FLRRNWPRIKKALEFLFTQDGDLNGLIEGEQHNTYDINFYGPNTMVGSLYLAACRAGE
                     EMARELGDAEFAARCRKVFEAGREATMKELFNGEYFIQKVDLKEHPKHQYADGCLSDQ
                     LFGQGWAHQVGLGYIYPPEAVKSALKAIWVYNWAPDVGPQNEAHPPQRWFARPGQAGL
                     FTCTWPKSKHLGPDSVLYRDEVWTGIEYQVAGHMVWEGMLLEALAICRGVHERYHPRS
                     HNPWNEVECGDHYARALASYGVFLALCGFEYHGPKGHIGFAPRMNADDFAAAFTAAEG
                     WGTFTQKRQGNTQENTLQVLWGRLRVNSIALEAPGENRVKAVVRIKSGTGEKEVGVSR
                     MEQTGRRVVIYLAVPLQLETGQTVTVHLT"
     gene            complement(4252..14934)
                     /locus_tag="PLD05_04800"
     CDS             complement(4252..14934)
                     /locus_tag="PLD05_04800"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOP76785.1"
                     /translation="MRVSSLFGFRQGGVSRGQQRKAKRSRRTRRQLLLEPLEQRHLLS
                     VVTITANDDPGGNGANNGIADLFRLVRNADDLEVYVNGNFVTSAPLAGLTAVQVGGSS
                     DADELQIDLSGGAFSTTIQFDGEALGLNVAPQDSLTLLGDPGAPIGRVTYAAGPLFGG
                     GADDGFIILDPDDNNGPGGGWNVGFATWNGDELMIQFANLSPVYDVVPAAQVDVFATH
                     AAETISILDGILVSGYQTAKVSASTFEDYFFANKTRVTVNSLNGGDTFILNYNVTVPG
                     LQLLDLYGNELTGGTLNPDDAQAETFQILATGPGTVANRMYGQRGAEYYTNSLTSYLD
                     PIQSNVQMHGGEGISIIDLSDVNDPTGDNIFIWGNKIVGAATGTIELYFATIEDIRFE
                     ATSGDDVITVLSTFADFQYRISGGPGNDTFNVGSYDTDPSPGVSLAWGPYGTGLNTIG
                     QIKGQLTIIPGPAWDAGDDTLYVDDSGGLAGYGAAMISDAAGGAVLGRTTTLLNFDAA
                     APIKYEYTPGGSELENLFIHTSQAADNLVVNATTARNTEIDLGPGSDFAVIAGDYVAG
                     ANVFKGGSGADTLWLHVLADLGGWAVLPLTQLQIEGNDPGGTVGGGDVVSVRELGVGV
                     PRALDFAYSGSGDVLLTGLAMPVDIRTAEVVEYLGGSGNDDTLSVTGTAADDLITVAP
                     LSGDAARVFLGGDPWDGPAADGDFFDQFPGVAGGGAGPDLHIAGAANLYVDAAGGSSN
                     RLYIYAPSEDDLTDGASVIVPGLGSGNAYDEISVTDSSVTINNNSLGALLPITFDSAA
                     LVQAIPTDRGLVVNAGFEATPDAAGLADQIYAMPSSLFAIMVNGGDPVPAFAPNGDRL
                     DIGGWGDVDIWADKNGTVSVAISGVGTFPVDFSSIENLAFWVWGAVNIYGDNNDPGVD
                     QNDLFEIVGTGYHSFALSINGNAPIQFFGVTDLNIFGDDATGTPSVGPNDVDTLVITP
                     FADNSPQGWGINISFDEGNPTDGTSTVPDLIIYNGIAGVSERIELVPTGPGDGQLRVT
                     NAADGSLVTVINYISNTGFIINGNDGSAGDTDSLTLHGTSGDDAIHIVDAQNANVSGI
                     YSVQFTNFAVLNVAGGDGYDTFHVNPSTLQVVIDGGTPDGVIQSDGDHLYVWPGLASY
                     HLILGPEGDNGTVAVTGAAPITFDRIEGLGFQGILYTLPDLNEPNDTIAEATILGSIP
                     HLTLRDLTIHTNEWNTTNDDYFQITARDSGALLVNVMFTDDGIGGNQVAGALSAELLD
                     RLGNVIATAVPTAYGVQIVAPVVSQQRYYVRVFSTDNDPNTYTLEIENFAAPAPTAIV
                     MHPAYDTGRSDSDGITAADDPVMLIQAWLSDLAEKGVPVLSAADVAAGLPGLAVEVFV
                     NGISAGFADPFGPSNNVFAFALPSGLLSAGLPGGYAGNPTAFGWLNLISAAVRVFDAQ
                     TVPASATRELGIPLPVTYDPNVPDASLLTLALATSCDSGVVGDGVTNIRTPTFQGVSE
                     INTIVRLYANGQLVGQATTGSDLSDGILGNGLGVFSITAAPLTDGVYFYAVTVEDVAG
                     NITGIVDSPIVILQIDTTPPQIPTIDLLDQFDTGWSDKDNVTKGDLTQGPGVVDVRVS
                     GELGTTAVIKDGETVIDTFVFAAAFMTRTLTLGEGPHPLTIEVTDTAGNRTQSEQLLI
                     TVDTIAPDPASISLAPYSDSGIPGDGITNVSAPAFVGLAEANARVRLYVDDGTGPVLA
                     GWTIVQSDESDGDPTNGLGVWEITVEPLADGEYTVWVEVEDLAGNISDQSEAIEITID
                     TTSPQRPTLDLQDADDTGWYDLDNVTIGDPLQLPAMGIVDLRISADPSSLVIIKDGNT
                     PVAAFVFDLAFDSTDGVIDGFGLYTISFPVNEFLFGIPAEGPHPLSVESVDAAGNVAQ
                     SEQLLVTIDYTPPAASAPALAGYSDSGISGDGITNVSAPAFVGSAEANARVRLYVDDG
                     TGPVLAGWTIVQSDESDGDPTNGLGRWEITVEPLTDGTYVVWVEVEDLAGNISDEQDN
                     LALGTEVTIDTTPPQIPTIDLVDQYDTGWYDKDNVTTGDPNALGTVRVRISAEYGTLV
                     LIKDGEVVIDSFVFDAAFDATDGVPGDLFGVRTIDFVLNQALFSIPAEGPHPLTVEAV
                     DVAGNFRQSEQLLVTIDFTPPLAPSAPDMLPDSDTGAFDDDNVTSIKNPAFWGVGEPN
                     SLVRIYVVNSLGQERLVGQATVGSDESYGDPTDNRGIWEVTIEPLDDGVYDVFAELED
                     QAGNISVWSAGLRIEIDTYAPNTPYLDLITADDSGRSNVDNITRVNTPNFTAATSDPN
                     ELIHLFAANLRYRVYDRTEGSGDVLIYDTGALVTSNSITTGALGPLADGVHNLKLEVE
                     DRAGNISADFLLTIVVDTEAPPAAFGLFTEVTDGLHPHSDTGLVGLPALADDRITSDT
                     TPTVWGQAEANSIIRVFVDANRNGRLDGTDVLLGQTTAVPLDGNQAEPFGYWEITSVV
                     DLNDPLIFAQRDGARTLFMQAVDVAGNESEVQRLDIFLDTRGPRVESVFITSAPAFDL
                     FNPKPSDGPTPLVDQLSIVITDGPDRAVGWLYNALALNGAGEFAVDPGHFSLVGDANG
                     VIPIAGITFVADPLVPGAPARGTLVLTFAEPLPDDRFTLTISDELRDPAGNRLDGESN
                     AVEPHETPSFPSGDGMPGGRFVARFTVDSRPEVGVWAAGSVWVDTNGNEVFDPENLDF
                     TNRDIVYAIGLTSDDIFAGNFALSPGDVADGFDKLAAYGRVNGAFRWLIDTNNDGVPD
                     INLVEPAAVNGLPIAGRFDNNDANGDEVAVVTAFPPAGEPSYWYFDTDHDYQVDNMLT
                     SQLRGYPVVGDFDGDGFDDLATWMDDTFYVDLANGVRRGWDGVADYTFRFGFIGIRER
                     PVVADMDQDGFDDFGLWVPDRTGQINDTTGEFYFLVSGGQSVLNRIVNDPIKGYPKID
                     FVPKPFGNDIFIAFGNQYALPVVGNFDPPVVPMATTTTNLMGTDGDDVLEFVAGDTAA
                     NWVVRLNGIVQTLDPATTDLVVDLGGGNDQVTFTGAAGDDVIDVSPGRFVFTGTGYTV
                     TVLNAEVLQADAGGGSDSANFTGTDGDDTFTARPGSAQIIAAGLNAQVNNVETIRATA
                     GSGGTQDVATLYDSAGDDVGQITPTGAVLSGTGFRLEASGFDFTHLVAKNGGNDAVLF
                     VGTAGQDELVARPDYSTMTLADGTFYRAKGFKAVEIAAPAGNQSSIKFVDSAGDDTFQ
                     ADPTTATLRGAGYEIVAHRVAYVHAYSRNGGFDSAYLYGSDGDDRIVVDQQFMKIYGD
                     SYLLRAKGFELTIADALDGADSAKVLDTAGDDTVTAEPYTFRLQTRKQEFRGYGVETQ
                     DIRSIYGGTDTVSLYDSTYDDVLTAQPNYAGLTGPGYAITVRAYDSVYAYSRSGHDIA
                     HLTGSSGDDQFVGDAVSGRLSGLGFLLRAKNFSEVYVQSGGGRDSAVLKATAAVDQIF
                     ANGLDAVLENATFKYALRAFSRVQVNGSGANDLAEVWNGQILHQEYDLDGNPLPEPDV
                     AVMVTLNDVAQVRVRSDVPPQDETVDGLGQTFKFFW"
     gene            15688..16701
                     /locus_tag="PLD05_04805"
     CDS             15688..16701
                     /locus_tag="PLD05_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76786.1"
                     /translation="MARYGVGMLAFLAFCILPIGSIWGQSACQPACAPACQPACQPAV
                     TCVQKTIMVPQWVVEMRKVRCIERVPEEREITVNVVKWVPETKMVERTRTQWVLEART
                     KEVKCVTYQPVWKTEVCEYIVMVPKIEKVQGVRKVCKMVPVTETRKVCVDEGHWEERP
                     CEPVCGICRHCHRRVRVVQSAACCPPANACQPACAAPKPVCVCKVWVPNPVVKEIQVT
                     CMKPQWVEEPCEYTKCTWVPEKRTREVKRCVWERQESVKQVTYSVCVPRTVVEKVPVT
                     VCKKVCEPVVKKCTVMVPRCVEKEVPVCVRKMVPTTITVPVRPCGLHHRVALRRAAPC
                     CDP"
     gene            complement(16950..18836)
                     /locus_tag="PLD05_04810"
     CDS             complement(16950..18836)
                     /locus_tag="PLD05_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOP76787.1"
                     /translation="MGRKLVFGTLVCLVIIMADLPMRCAPSGFGELFASDLRQAWESP
                     APHEVQLGGEIGRRIRCTIEENLLKLDLDRDFLQAFRVRNKKDGYVGLGKLLDSIVRL
                     AAHTKDARLIEMKDRIVGELCQLQEPDGYLGIMEPQSRMWKLWDIHEMSYLVYALASN
                     HRFFKEQRSLECGKRLADYIIRNWEAHPEAKPGGGSITVYMAVTGLENAFLLLAEETG
                     ETRYRDFVVNFRQLPEWDARIVVGRHGDIEGHIYAYLCRSIAQLRLSSVYPEERLWRA
                     SRQALDFLLHKEGLVITGECGDHECWHDTQAGTINLGETCATAYLIRWLDELLRREKK
                     SLYGDLMERAIYNGLFAAQSPDGRSIRYYTPFDGPRKYFPSDTYCCPNNYRRIIAELP
                     NFVAYATPEGIAVNLYTEGRVTLHLPSGQRVAVIQRTDYPSDGRVTIALDLGAEESFE
                     TRLRIPKFCDHARIDLPGGESLEAKGGDWAVLKRVWKPGDVVTLNLPMGLRFVKGRRA
                     QVGRVAIMYGPLVFCLNRKDHPDLKDVDLRLLTIVPESLTGPFRDDSVRPGGLACEVK
                     AWSPGAWYPQAQPAYTLRLTEFPDPSGEAIYFHVPNPYDVKFVDDELITPVDVEDISS
                     VAQR"
     gene            complement(19220..19717)
                     /locus_tag="PLD05_04815"
     CDS             complement(19220..19717)
                     /locus_tag="PLD05_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76788.1"
                     /translation="MSTASTIARYYDSLMEEAPPLLEWDDWPIRDDWSVLIVFLLALA
                     GVSLAVFWVTERFLWTAIVDILLIASFARALLKATYRMGPYGVERIVFGRLQRVPWRA
                     IRAIQIEDHGVFLFDTHNPCPADALRAVFVPFGPHRDAVLARLEYYLAQGTRGTESLS
                     QAAIL"
     gene            complement(20188..21603)
                     /locus_tag="PLD05_04820"
     CDS             complement(20188..21603)
                     /locus_tag="PLD05_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP76789.1"
                     /translation="MRAALLLLGILAVGVVELSAVAADRPNVLIIMADDCTYNDLPVY
                     GGENAYTPNIDRLAKEGLVFDRAYLAMAICQPCRSELYTGLFPIRNGCAWNHAASRPG
                     IQSLPHYLGRLGYRVGLAGKVHVTPKEVFPFENVPGFCPNCTADPTPPHDLAGIREFM
                     TRKKDQPFCLVVALVEPHVPWVMGDRSRYPVKKLRLPPHIADTPKTREDFASYLAEIT
                     YMDGQVGEILGVLDEAGLREHTLVLFTSEQGAQFPGCKWTNWDLGVHTALIARWPGRV
                     PAGKRTPAIVQYADVVPTLIELAGGQPKEMGDFDGTSFVEVLLGRKDSHRTYAYFVHN
                     NFPEGPPYPIRSVSDGRWHYIRNLTPQEMYIEKHLMGMLGGSVPHNAYWPTWIAHSWN
                     RPDVYRLVKRYCVRPAEELYDTSADSYEMTNLADRPEVAEIKKRLSEELDRWLAEQKD
                     PGISVDTPEAYQAMKDGKPQF"
     gene            complement(21769..25146)
                     /locus_tag="PLD05_04825"
     CDS             complement(21769..25146)
                     /locus_tag="PLD05_04825"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOP76790.1"
                     /translation="MRELTRGVCFGTFLALGSAIFALGDEPLLVLEDVPSGGMVCVNV
                     DLGPVLKRCGLSRVAWQNLRVFTEDNVLVPAQFIPEPFTRSNTSATGAEFSGAGILIL
                     RLPEPPPTRLVLRSEQTADSDSDSAQAFDEKVSMPYLSVEHNAAYGGFFQKVWIGQSA
                     LEFPVRFADRVHHTKEGSFSAAGYSSAQVKLLSRGPLGVHVRTQTRYVRGEQSPTSQP
                     QACYDWFYFYDLPVIWVRVVATQAEGFRWNEHHFLEIQFSENTANRWMGGEPLQEGTL
                     TRTAKSHPLTHIGMVRNEQVGLGIFCGGSVLVYDSPGGDGHYIQAHGDRAWQPWETAK
                     ITKEAYIFVGEQGDFDGRIREVPGLVPKQVDAIVTTREIHDRLAAARAKLSPLLLAAT
                     SYCERTGHWEDALRAAEGTMPEFLTGIAAGNLSLVMEKLPNGLKGPVAYDVRQDRLFV
                     REDLPLFCIRARHAVSKEELVLSADAGWNKVTCQLGDGHSSLKVRWQDFSDARLKGIA
                     VEVEAIAEESRQAIRWRLKVENESTEWSIWSVVFPQVAIAEPGRDAWVVYPKGVGIAE
                     QGAWERALQFRGRYPSGWVTMQFVGAYAADNSTGLYLAVHDPWGSTKEIQLQSRPEDH
                     IVTWAVEHPAENMGQAGNHFELPGEAVFQAFAGDWFDMALIYRDWVRREARWYPKLGA
                     DGREDTALWMRELCLWALGGGAPADCVERVKRFAEYFGVPCGFHWYNWHQIPFDNDYP
                     HYFPTKEGFAEAVAELQKSGVHVMPYINGRLWDTRDRGLEDFEFSSRAKPAVSKNEVG
                     EPYREQYSSKESDGSPVSLGVMCPSTSLWQETVQGIVARLFSECGVHGVYIDQIAAAS
                     PTLCFDASHGHPLGGGHWWTTSYWQLLEGIRKAMPSERMITTECNAEPYIRWFDGYLS
                     WHWQYPNQVPLFPAVYGGTIQMFGRYYGGTGSGLRPTEPAGRNRDIALRMRASQQLVF
                     GEQLGWINPAVVDEPQNASFLKKLVLLRSQAVRFFYAGEMVRPPRVAGNIPQLRADWQ
                     WQGSMWVTTDAVFTGAWAIPRERKAIAFMVNAAEEELTVRVEADTRQWGLNASKIRTT
                     CVLPEGRQQTEVGPSTWAVEMTLPPCSAQAWEFEAIE"
     assembly_gap    25820..25919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26181..26333
                     /locus_tag="PLD05_04830"
     CDS             26181..26333
                     /locus_tag="PLD05_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76791.1"
                     /translation="MPGLSPGLPRSAGKRDHGPNGKFSFPVMATWLGHTEAIAEAHYW
                     QTTEAH"
     gene            27338..27919
                     /locus_tag="PLD05_04835"
     CDS             27338..27919
                     /locus_tag="PLD05_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76792.1"
                     /translation="MRVSDLGLPATAIAPVVTPNNGKTNQGQQTSEGATSQRLQPPQG
                     DQAPKPLHRLAQVRIRQGVSRRTMARRMKVDIRTVREEEDPTRDLPLSALYRWQRALQ
                     VPLIELLSDIEEPLSAPVLKRARLLRMMKTVRAIQLRAKQPVVRQLAELLAEQLLEIM
                     PELADVSPWPMGRRPRTSDCGPTADRIFKVRFR"
BASE COUNT         6360 a         8341 c         7505 g         5767 t
ORIGIN      
        1 agcggcttgc ccgatcccaa cttgaccatt gtttgcagaa ctcgccgtcc tccgggagga
       61 ccgctgccgc ccgaggatcg accatctgca tgagtaagtc gcgataggtg tacgtggttt
      121 cggccagggc gtgaaaccct acagccatcc cgaccgttcc cagaagcgcc aaccaaatgc
      181 gattcataga accctccgca ttgtgtcaaa gacctaatga cgtgaccacc acgaggcgat
      241 cgtaaccttc gatttagagc ctatcccaaa ttccctctcc ctttgggaga gggttggggc
      301 gaaggtaagg catcgtcgga gtcgatcgag aaaaccagca gcgattcgag ccaccctcac
      361 cccgtcccct ctcccgccga acgggcgagg ggacgttgtg ggatagcctc ttatccacct
      421 tcttcaccgg cgatctcttg gacgatccaa tcttccccta accacaaacc gatgaccgtc
      481 atggtgggaa acctttgccg aaggaattcc acggcagccc gaatttgccg atgatgttcc
      541 tccggcgaga cgggattccc cccgcaatcg gcgtgcccga caatggccag ccatcttgag
      601 ccgtgtttct cctgagaaat cctaatcttg cgacaaatgg cctcaaccgt agcggggtca
      661 tcgaaccggg ctaaaaagcc catcggggcc cgcttccgtg ctgttatcca cataatccac
      721 accgaaccgt tctttaagat aactgatcac aggcccttgt gttcggccat ccatgcagtt
      781 gacaacagtg gcaaaactca tggcctttag ccctttccaa gatacctgct gtcgcttaat
      841 cccgatcaag tcaggtgaac agtcacagtc tggccggtct ctaactggag aggtactgcc
      901 agatagatca ccacgcgccg gccggtttgc tccatgcgag aaacgcccac ctctttctcg
      961 cccgtgccgg actttatccg cacaacggct ttgactcggt tctcgccggg ggcttccaag
     1021 gcgatgctgt tcacgcgaag gcgtccccac aggacttgaa gagtgttttc ctgcgtattc
     1081 ccttgacgtt tctgcgtgaa cgtgccccaa ccttcggccg ccgtaaaggc tgccgcgaaa
     1141 tcatcggcat tcatccgcgg ggcaaatccg atatggcctt tggggccatg atactcaaac
     1201 ccgcacaggg cgaggaatac gccgtagctg gcgagtgccc gcgcgtagtg gtcaccgcat
     1261 tcgacttcgt tccacggatt atgactgcga ggatgatacc gctcgtgaac accgcggcaa
     1321 atcgccaagg cctcgagtag catgccttcc cataccatgt ggccagcaac ttgatattcg
     1381 atccccgtcc aaacctcatc gcggtacaaa acgctatctg gtccaaggtg ctttgatttc
     1441 ggccaagtac acgtgaaaag ccccgcctgt cctggtcggg cgaaccaccg ctgaggcgga
     1501 tgtgcctcat tctgcggccc gacgtccggt gcccaattgt aaacccaaat cgcctttaac
     1561 gcggacttga cggcctcggg cggatagatg taaccgagcc ccacttgatg ggcccagccc
     1621 tgcccgaata actgatccga caaacatccg tccgcgtatt gatgcttggg atgctccttg
     1681 aggtccacct tttggataaa gtactcgccg ttaaatagct ccttcatggt ggcctcgcgg
     1741 ccggcttcaa ataccttacg acaacgcgcg gcgaattccg cgtcgccaag ctctcgggcc
     1801 atctcttcgc cggcacggca cgccgccaaa tagagcgaac cgaccatagt gttcggccca
     1861 taaaagttga tgtcgtacgt gttgtgctgc tcaccctcga ttaggccatt gagatcgcca
     1921 tcctgggtaa agaggaattc cagggccttt ttgatacgtg gccaatttcg ccgcagaaag
     1981 ccatcatccc gcgagcattg gtgttcccga taggccttga ggatcgtacc gccttgggaa
     2041 tcaccggccc aaagcttcca gccttcacct cgaaagcgaa ccgcaccggt ctgctcgtca
     2101 aaacctgcct ccggattgaa atcctgcatc tcgcggacgg accgttccaa ccgagggaat
     2161 aggcgggcca aggcgtgttc gtagttccac acgtgggcac acgtcccgtg acagcaaccc
     2221 acaccctccc acgcccaaaa ccggccgttc gcccaccact gacatgtccc ggtggcaagg
     2281 ttggataccg tggaaaacag tcgatccagt agccaatgcg gcagtgtcgc accataccag
     2341 gtggcatacc acaatttcgt ctgttcggtc aaacgaggga gatttgcctc caaatatcga
     2401 gctaaatcgt ccagtgtggg aaacttcacc gcgtagtagt tgccgcgttc tggccgattt
     2461 gggaaatgcc acaaaaccgc gaaagtcaac tgggcggtct ttccaggggg gatgctaaca
     2521 cgacacccca gcgccccccg aagtttttcg tggagccgtt gttttcctag ggtagtttct
     2581 ccttgcaaag gattgaattg gtgccacagc gtctccacat caagcggtcc accgccatcg
     2641 gccaccgcga ggagttcgta cttgccgact tcgtcggacg ccggtggtaa aaagaagacg
     2701 agggccattt ggccgaaatc gggttgcttt tccaaaggtc cctcgggagc aggtcgcggt
     2761 gtatcccgaa gttcgatctg atccacattg atatgccccc agccgccgga ttcccgatcg
     2821 acgatctcaa tttgcgccgt cttgccctca aattcacgaa cgtcccagtt ctgccactgt
     2881 aaccgctcat tgttggtgcc cgtggccgtt cgcaccactt ggccgtccac taacagattc
     2941 atgcatgtct ttccggcgtg gcctccgcca ccgataagaa agctgatata ccgtcggtcg
     3001 atcacaaagg gtggcgagac aagtcggcca tgcggtcgat cgcctcccaa atatgtgtta
     3061 actagcccct ttcccaggaa accgctcacc gtttgttgat tggggagcgt tccgcgagcc
     3121 ggtccttggc cgaaggcctc accctccacc gtccaattgc cgtaattttc cccttcaaag
     3181 tcggcaagaa ccttcaccgg ccggggttcc tctggcggtg gtgagggctg gacggaatga
     3241 actaaagcta ctgactgaga gtatcgcttc gcatgagtaa gccgttcacc ccaaaaagaa
     3301 ataccggtgt ggcacgccac ggcgttttcc aaccagccca tcaatgttac ggtcttggtc
     3361 tcgggaccgg gattcttgat tgaaaaggcc aagatcgtgg ccggcagtgc cgaatcctcc
     3421 tcgttgagag ggataaacgg cgaaaaggcc tccagccgga cttgcacggg aaagttagga
     3481 tcatgatagt ccacataacc gatagggtat tcaccgcaga aacgaacctc tggaaaacct
     3541 gcctgatcca aggtccggcg aatctctttt ccgtccgttt gaaccgaaat cgcaaatccc
     3601 tgctccaccg gataatccgg cggcggcatc tgttgataat tgttggcccc gtagccggag
     3661 aagttatgtc ggttaaaaat gtcccaatga aacagtcggc catcccccgc aaggtaaagc
     3721 tggcccgcac aaattccgcc gataggcatc ccgatgtttt ttaggtcttc gccggaatac
     3781 cacgtggatt caccacgagc gaaaagttgg gcgatccact ccgggctcag tttcttatca
     3841 acgggaacga aatggtcggc ggtatcggct ggctcaaagg gacctgcaac gataggccct
     3901 ttccatgcgg ccaatgcggc cgccgttgta gccaattgca gaaatgttcg acgtggcaaa
     3961 cccgaggctt gacagccaca gtcgtaacct cgccgtgatt gcgggacgtt gcgagtgtca
     4021 cacatatttc ggctccctgg caactggaca tgtcacatgg tgtcggggat gcgaatgaca
     4081 ttctacggtg cccgcatcgc attcacaaca atgaccgacc acaaaatgcg agacctgact
     4141 gagaagaata aacaaaaaac gccgtgccag atttcccagc acggcgtaac gaagcttgct
     4201 gtcatggcct ggccctaagc acgagccagc gtcacttgaa agctccgaag attaccagaa
     4261 gaacttgaag gtttggccaa gtccgtccac ggtctcatcc tgaggtggaa catctgatcg
     4321 tacacgaacc tgtgccacgt cgttcaaggt gaccatcacc gcaacatcag gttccggaag
     4381 cggattgcca tcaaggtcat attcctggtg gagaatctga ccgttccaga cttccgccaa
     4441 atcattggcc ccgctgccgt ttacctgcac acgggaaaat gctcgcaggg catatttgaa
     4501 cgtggcattc tctagcacgg catccagacc attggcgaaa atctgatcga ccgccgccgt
     4561 ggcctttaat acggccgaat ccctaccgcc accggattgg acatagacct cactgaagtt
     4621 ctttgcccgc agcaggaacc cgaggccgga gagacggccc gaaacggcat caccgacgaa
     4681 ctggtcatcg ccactgcttc cggtcaagtg agcaatgtca tgtccgctcc gcgagtaggc
     4741 atagacggaa tcgtaggccc tgacggtgat tgcgtaaccc ggccccgtga gacccgcgta
     4801 gttcggctga gctgtcagca catcatcata ggttgaatcg tacagactga ccgtgtcggt
     4861 tccaccatag atagaacgga tatcctgggt ttccacgccg taacctcgga attcctgttt
     4921 tcgggtttgc aggcggaatg tataaggctc agccgttacg gtatcatcac cggccgtatc
     4981 gagcactttg gctgaatcag ccccatccag ggcatcggca attgttagtt cgaatccctt
     5041 ggcgcgtaac aggtaggaat ctccgtagat tttcatgaac tgctgatcga cgacgatgcg
     5101 gtcgtctcca tccgacccgt acagatacgc cgagtcgaag ccaccgttcc gggaataagc
     5161 atgcacgtag gcaacccgat gggcaacaat ctcatatccc gctccacgca acgtggcggt
     5221 cgtcggatcg gcttggaacg tgtcgtcgcc ggcgctatcg acaaatttga tcgagctttg
     5281 gttgccagcc ggggcggcga tttccacggc cttgaagccc tttgcccgat agaacgtacc
     5341 atccgccagg gtcatcgtgc tgtaatcggg acgagctacg agctcatcct gtccagcggt
     5401 gccgacaaat agcacggcgt cgttgccacc gttcttggcc acaagatggg taaagtcgaa
     5461 tccgcttgcc tcaagccgga aacctgtccc gctgagaacc gcaccggtcg gcgtaatctg
     5521 ccccacatcg tcacccgcgg agtcgtagag cgtggccaca tcctgcgtac caccacttcc
     5581 agccgtcgcc ctgatggtct cgacgttgtt cacctgggca ttcaggccag cagcaatgat
     5641 ttgggcactt ccaggacggg ccgtgaacgt gtcgtcacca tccgtgccgg tgaagttggc
     5701 cgaatcactt ccgccgccag cgtcggcctg gagaacctcc gcgtttagca ccgtcacggt
     5761 gtagccggtc ccggtaaaca cgaagcgacc cgggctgacg tcaataacat cgtcgcccgc
     5821 tgcccccgtg aaagtcacct ggtcgtttcc gccgcccaaa tcgaccacca ggtcggtggt
     5881 ggcgggatca agtgtttgca cgatcccatt gagtctcacg acccaatttg cggcggtatc
     5941 gcccgccaca aactccaaga catcgtcgcc atcagtaccc ataagattgg tggtcgtggt
     6001 ggccattggc accacgggcg ggtcaaaatt gccaaccacc ggcagggcat actgattgcc
     6061 gaaagcgata aagatgtcat tgccgaacgg tttcgggaca aagtcgatct tgggataacc
     6121 cttaatcgga tcgttgacaa tccgattcag caccgactga ccgcccgaca cgaggaagta
     6181 aaactctccc gtcgtatcgt taatctggcc agtccgatcc gggacccaca ggccgaagtc
     6241 gtcgaagccg tcttgatcca tgtcagccac gacgggccgt tcccgaattc caatgaagcc
     6301 gaagcggaag gtgtaatcgg cgacgccgtc ccaaccgcga cggacaccat tggccagatc
     6361 aacatagaac gtgtcgtcca tccaggtggc caggtcatcg aagccgtcgc cgtcgaagtc
     6421 acccaccaca ggatagcctc gaagctgact cgtcagcatg ttatcgacct ggtagtcgtg
     6481 atctgtgtcg aaataccagt acgaaggttc tcctgccggc gggaaagccg tgaccacggc
     6541 cacttcatcg ccgttagcat cgttattatc gaaccgtccg gcgatgggca gtccgttcac
     6601 cgcagcgggc tccacaagat tgatgtccgg aacaccgtcg ttattggtgt caatcaacca
     6661 tcggaacgcg ccgttcacgc ggccataggc agccagttta tcgaatccgt ctgcaacatc
     6721 gcccggactg agtgcaaaat taccagcaaa gatatcatca ctcgtcaaac cgatcgcata
     6781 gacgatatcg cgattggtaa agtctaggtt ttcgggatcg aagacctcat ttccattggt
     6841 atcgacccag actgatccag ccgcccagac acccacttcc ggtcggctat ccacggtgaa
     6901 gcgcgccaca aacctcccac ccggcatgcc atcgccgctg gggaaggaag gcgtctcgtg
     6961 tggctccacc gcattcgatt cgccgtccaa gcggttgccg gccggatccc gcagctcatc
     7021 ggaaatcgtc agcgtgaagc gatcatccgg caacggttca gcgaatgtga gcacaagggt
     7081 gccacgcgcc ggcgcgccag ggaccaaagg atcggccaca aacgtaattc cagcgatcgg
     7141 gatcacgcca ttggcatcac caaccagcga gaagtgccca ggatcaacgg cgaactcgcc
     7201 agccccgtta agggctaaag cattataaag ccatcccacg gcgcggtccg ggccatccgt
     7261 gatgacgatg gaaagctgat ccaccaacgg ggtcggccca tcgctcggct ttggattgaa
     7321 gagatcgaat gccggcgctg aggtgataaa gacgctttcc acacggggtc cccgcgtgtc
     7381 caagaagata tcaagcctct ggacctcgga ctcatttccc gccacatcta ctgcctgcat
     7441 aaacaacgtt cgcgcgccat cccgttgggc gaagatcagc gggtcattca aatctacaac
     7501 cgaagtgatt tcccagtagc cgaagggttc tgcctgattg ccgtcgagcg gtacagccgt
     7561 ggtttggccg aggaggacat cggtcccgtc gagtctgcca ttgcggtttg catcgacgaa
     7621 aacccggatg atgctgttgg cctctgcctg accccaaaca gtgggcgtcg tatcgctagt
     7681 gatacggtca tcggcgagag ctggtaaccc aacaagcccc gtatcgctgt gaggatgcaa
     7741 accatctgtg acctctgtga aaagaccaaa cgcggctggc ggcgcttccg tgtcgaccac
     7801 aatggtcaac aagaagtccg cgctgatgtt gcccgcacgg tcttcgactt ccagcttcag
     7861 attgtgcact ccgtcggcca acggtcccaa tgcgccggtg gtaatcgaat ttgaagttac
     7921 tagcgcacca gtatcgtaga tcagaacgtc accgctgcct tcggttctgt cgtaaactcg
     7981 ataccgcagg ttcgcagcaa acaagtggat aagttcattg ggatcgctcg tggccgccgt
     8041 gaagttcggt gtgttcaccc gcgtaatatt atccacatta ctccgaccgc tgtcgtcggc
     8101 cgtgatgaga tctagatacg gcgtgtttgg ggcataggtg tcaatctcga tcctcaagcc
     8161 cgccgaccaa acactgatgt tgccggcctg gtcttccaat tctgcaaaga cgtcatagac
     8221 accatcatcg agcggctcaa tggtgacctc ccaaatcccc cggttatccg tcggatcacc
     8281 atagctctcg tcagagccca cggtagcctg ccccaccaat cgttcctggc caagactgtt
     8341 gacgacataa attcggacca gcgaattggg ttcgccaact ccccagaaag ccgggttctt
     8401 aatgcttgtc acattgtcat cgtcaaaggc acccgtatcg ctgtcgggta acatatccgg
     8461 cgcggaagga gccaacggtg gagtgaagtc gatggtcacc aacaactgct ctgactgccg
     8521 gaagttaccg gccacatcaa ccgcttccac cgtaagagga tggggtccct ccgccgggat
     8581 tgagaagagg gcttgattca acacgaagtc gatcgtccgc acgccgaaca gatccccggg
     8641 cacaccgtcc gtggcatcaa aggcggcgtc gaagacgaag ctgtcgatca ccacctcacc
     8701 gtctttgatg agcacgaggg ttccatactc cgcagagatg cggaccctta cggtccccaa
     8761 ggcgttcgga tcgccggttg tgacgttgtc cttgtcgtac caacccgtat cgtactggtc
     8821 caccagatca atcgtgggaa tctggggcgg ggttgtgtca atagttactt cggtccccag
     8881 ggcgaggttg tcctgttcat cactgatatt gcccgccagg tcttccacct ctacccacac
     8941 tacgtaggtc ccatcggtga gtggctccac cgtgatctcc cagcgcccca gtccgttagt
     9001 gggatctcca tcgctctcgt cggactgcac aatcgtccat cccgctaaca ccggtccggt
     9061 tccatcatcc acatacaagc ggaccctcgc gttggcctcc gccgaaccaa caaatgccgg
     9121 ggccgaaaca ttcgtgatac cgtcgcctga tatgccagaa tcgctataac ccgcaagcgc
     9181 gggggcactt gccgccggag gtgtgtaatc gatcgtgaca agcaactgct ccgattgtgc
     9241 cacgtttccc gcagcatcaa ccgattccac gctgagggga tgggggcctt ccgccggaat
     9301 gccaaagagg aactcattga ccgggaagct tattgtgtag aggccgaacc catcaatcac
     9361 cccgtcggtc gaatcgaaag caaggtcgaa aacaaacgcc gcaacaggag tgttaccatc
     9421 tttgatgatc accaagctac tgggatcggc tgagatgcgc aggtcaacaa ttcccatcgc
     9481 cggcaattgc agcggatcac cgattgtcac gttgtccaga tcgtaccaac cggtgtcgtc
     9541 ggcatcctgg agatccagcg ttggccgctg cggagacgtg gtatcaatcg tgatctctat
     9601 cgcctccgat tgatcgctga tgttacccgc caggtcctcc acttccaccc aaaccgtgta
     9661 ttcgccgtcg gccaaaggct ccacggtaat ctcccacacc cccagtccgt tagtgggatc
     9721 tccatcgctt tcgtccgact gaacaatggt ccaaccggct aacactggtc cggttccatc
     9781 atccacatac aagcggaccc tcgcattggc ctccgccaaa ccaacaaatg ccggagccga
     9841 aacattcgtg ataccgtcgc ccggtatgcc agagtcgctg tatggagcga gactaatgct
     9901 ggcgggatcc ggagcaattg tatccacggt gatcaaaagt tgctcagatt gagtccgatt
     9961 tcctgcagtg tcagtgacct caatggtgag aggatgcggt ccctctccga gggtcagtgt
    10021 ccgcgtcatg aatgccgcgg caaacacgaa ggtatcgatg acggtttcgc catccttgat
    10081 cactgccgtt gttcccagct cgccagaaac ccgaacatct accacaccag gcccctgcgt
    10141 caaatcgccc ttcgtgacgt tgtccttgtc ggaccagccc gtgtcgaact ggtccaacag
    10201 atcaatcgtt ggaatctgag gcggcgtggt atcaatttgg aggataacga tagggctatc
    10261 aacgatgccc gtgatgttgc ccgccacgtc ttccacggtc accgcataga agtaaacccc
    10321 gtcagtcagt ggtgctgctg tgatagaaaa cactcccaga ccattaccga gtatcccatc
    10381 gctcagatcg gagccggttg ttgcctggcc cacaagttgg ccattggcat acaatcggac
    10441 gatggtatta atctcgctta caccttggaa cgtgggggtg cgaatgttgg taacaccatc
    10501 gccgacaact ccgctgtcac atgatgtggc cagcgccaac gtgaggagac tcgcatcggg
    10561 gacattgggg tcataagtta cgggaagagg aatccccagt tctcgtgtgg ccgaggctgg
    10621 aacggtctgg gcatcgaaca cccgcaccgc cgcactgatc agattgagcc aaccgaaagc
    10681 cgtgggatta cccgcgtaac cgcccggcaa gccagcagac aaaagtccgc taggcaaagc
    10741 aaaggcaaac acgttgttcg acgggccaaa tgggtcggca aagccagcgc tgatcccgtt
    10801 gacaaacact tcgacggcca gaccaggcaa cccagccgcg acgtctgccg cgctaagaac
    10861 cgggacacct ttctccgcca ggtccgacaa ccacgcctga atcagcatga ctggatcatc
    10921 ggcagccgtg atgccatcgg aatcgctgcg gccggtatca tacgccggat gcatcacgat
    10981 cgcggtcggg gccggggccg cgaagttctc aatctccaac gtgtaagtgt tgggatcgtt
    11041 gtccgtactg aagactctca catagtatcg ctgttgcgac acgaccggtg cgacaatctg
    11101 gacaccataa gcagtcggga cggccgtggc aatcacgttg cccagcctgt ccagaagctc
    11161 cgccgaaagc gcgcccgcga cctggtttcc accaatccca tcgtccgtga acatgacatt
    11221 caccaataag gccccactgt cgcgggcggt gatctggaaa taatcatcgt ttgtggtgtt
    11281 ccattcattg gtgtgaattg tcaagtcccg aagagttaaa tgtggaatgg aaccaagaat
    11341 ggtggcttct gcgatcgtgt cgttcggttc gttcaagtca ggtaacgtat aaaggatgcc
    11401 ttggaaacca aggccttcga tccggtcaaa tgtaatcggg gcggcccctg tcaccgccac
    11461 tgtgccgtta tcaccctctg ggcccaagat gagatggtag cttgccaatc ctggccatac
    11521 gtacagatga tcgccatcag attggatcac cccatcaggg gtgcccccat caatcaccac
    11581 ctgcaacgtg gacggattga cgtggaacgt gtcgtagccg tcgccgcccg ccacgtttag
    11641 cacagcaaaa ttggtaaact gaaccgagta gattccgctg acgttcgcgt tttgagcatc
    11701 gacaatgtgg atcgcatcat ccccggaagt gccgtgcaag gtcaacgaat ctgtatcgcc
    11761 ggccgaaccg tcgttgccat tgatgatgaa accggtgttg gaaatgtaat tgatcactgt
    11821 aaccagcgaa ccgtccgctg cattggtcac ccgcaattgc ccatcgccgg gaccggtcgg
    11881 aaccaactcg atgcgttctg aaacaccggc aattccgttg tagatgatga gatcaggcac
    11941 ggtactggtg ccatccgtcg gattcccctc atcaaaacta atgttaatgc cccagccttg
    12001 cggcgaattg tcggcaaacg gtgtgatgac gagggtatcc acatcgttcg gaccgacact
    12061 cggcgtgcct gtcgcgtcgt cgccgaagat attgagatcg gttacaccga aaaactgaat
    12121 tggcgcattc ccgttaatag agagcgcaaa cgagtggtag ccggtaccca caatctcaaa
    12181 gaggtcgttt tggtccacgc cgggatcgtt gttgtcgccg taaatgttga ccgctcccca
    12241 aacccaaaag gcgagattct cgatcgagct gaaatcgaca gggaaggttc ccacgccgct
    12301 aatggccacg ctcaccgtcc cgtttttatc tgcccaaatg tccacgtcgc cccacccccc
    12361 aatgtccagt cgatctccgt tgggtgcaaa ggcgggcacg gggtcgccac cgttgaccat
    12421 tatggcgaag agactggagg gcatggcata gatttgatcc gcaaggccgg cagcgtccgg
    12481 cgtggcttcg aatccggcat tcacgaccaa gccgcgatca gtcggaatgg cctgcaccaa
    12541 agctgcagaa tcgaacgtga ttggcaataa cgcccccaaa gaattgttgt tgatcgtaac
    12601 cgaggagtcc gtcacggaaa tctcatcgta ggcgtttcca gaacccaacc ctgggacgat
    12661 gaccgacgca ccatcggtga gatcatcctc gctgggagca taaatgtaga ggcgattgga
    12721 ggatccgccc gcggcatcga cgtacagatt cgccgctccg gcaatgtgga ggtccggtcc
    12781 ggccccgccg cccgccacac cggggaattg atcaaagaaa tcaccgtcgg ctgctggacc
    12841 atcccacgga tcgccgccca gaaagacccg cgccgcatcg ccgcttagcg gtgccaccgt
    12901 gatcaaatcg tcagccgccg tgcccgtgac gctcagcgtg tcgtcattgc ctgaacctcc
    12961 gaggtactcc accacctccg cagtgcggat atcgacgggc atggcaagcc ccgtcaaaag
    13021 aacatcaccg ctgcccgaat acgcgaaatc cagtgcgcgt ggaactccta cacccagttc
    13081 gcgcaccgag acgacatccc cgccgcctac tgtcccgccg ggatcgtttc cttcgatctg
    13141 gagttgggtc agcggcaaca ccgcccagcc ccccagatca gcgagcacgt gcagccatag
    13201 agtatcggcg cccgatccac ccttgaagac gttcgcgccg gcaacatagt caccggcaat
    13261 cacggcgaag tcgctgcccg gcccaaggtc gatctccgta tttcgcgccg ttgtggcatt
    13321 gaccaccaaa ttgtccgcgg cctgcgaggt gtggatgaaa agattttcca actctgaccc
    13381 gccgggcgtg tattcgtact taattggcgc cgcagcatcg aaattgagga gggtcgtggt
    13441 ccgacccaag acggcaccgc ccgccgcatc gcttatcatg gcggcaccgt agcctgccaa
    13501 ccctcctgaa tcgtccacgt acagcgtatc atcgccagcg tcccacgcgg gtcccgggat
    13561 aattgtcagc tgacccttaa tttgtccgat cgtgttcagt cccgttccgt acggtcccca
    13621 agccaaggag acaccaggcg agggatctgt gtcgtaagaa ccgacgttaa atgtgtcgtt
    13681 accaggccca ccggagattc ggtactgaaa atcggcaaac gtggaaagca ccgtgatgac
    13741 atcgtcgccg gatgtcgcct caaaccggat gtcttcgatc gtggcaaagt acagctcaat
    13801 agtgcccgtc gccgctccca caatcttgtt gccccagatg aagatgttat caccagttgg
    13861 gtcgttaaca tcggaaagat cgatgattga aataccctct ccaccgtgca tttgcacatt
    13921 actttggatg ggatcgagat agctggtgag actattcgtg tagtattctg caccgcgctg
    13981 accatacatg cgattcgcaa cggtgcccgg gccagtcgcc aggatttgga atgtctcggc
    14041 ctgggcatca tccgggttga gcgttccacc ggtcaattca tttccgtaaa ggtccagcaa
    14101 ttgaaggccg ggcacggtga cgttgtagtt aagaataaac gtgtctccgc cgttgaggct
    14161 gtttaccgtc acacgggtct tgttggcaaa gaagtagtct tcgaacgtcg atgcagaaac
    14221 cttagcggtt tgataaccac tgaccagaat gccatcaaga attgaaatcg tctcggcggc
    14281 atgggtcgcg aagacatcaa cctgggcggc tggcacaacg tcgtacactg gcgagagatt
    14341 ggcaaattga atcatcaact cgtcgccgtt ccaagtcgca aagccaacgt tccaacctcc
    14401 gccgggtccg ttgttatcat ccgggtcaag aatgatgaac ccgtcgtccg caccaccgcc
    14461 gaataatggc cccgcggcgt aagtcactct gccgataggg gcaccagggt cacccaaaag
    14521 cgtgagcgaa tcttgggggg caacattaag tccgagggcc tctccatcaa actggatcgt
    14581 cgtcgaaaac gctccaccgg agaggtcgat ctgtagttcg tctgcgtccg aggaaccacc
    14641 gacttgcact gccgtcagcc cggccaaagg agctgaagtg acaaagttcc cgttgacgta
    14701 aacttcgaga tcgtcagcgt tgcggaccag ccggaataaa tcggcgattc cgttattggc
    14761 accatttccg cccgggtcgt cgttggccgt aatggtaacc acactcaaca gatggcgctg
    14821 ttccaagggc tccaaaagca actgccttcg cgttctgcga gagcgtttgg ccttcctttg
    14881 ttgacctcgt gaaacaccgc cttgccgaaa accgaagagg ctgctaactc gcatggttgc
    14941 tcactccaca aaagaaatta ggccgctgct aaaaaccccc ctgaaacgca gacttggtca
    15001 aaactcaacg tcaaccgcga ttgtgttccg ttcgaaaagc gagagtggcg acttgcccaa
    15061 aaaacttctc aagtcgcgca aatccttaaa atccgcaaag tcgacaaagc tcctcaaatt
    15121 ggcaaacctg acaaaattta taatctgcgg cacccccgtt gtcaacaact ttcttggcca
    15181 gccccgaatt agggcgaata ccggaaaaac caggcccccg tccattgggg tatatgcccg
    15241 agttggcctg ctcagcggag ggaccaaaaa aacctttggt gtccgttctc tcagggcacc
    15301 tcttttggct gcgactgtaa agacaaaaaa cgactcgcgc gaaccagcat ggcgaataga
    15361 atggcggact tcaagtgaag gccgtgctgg ggaaaaaccg gggcccgtaa tgcgttgcgg
    15421 gggcttgacc cgcccttgac cctccgataa catcaagctg gacaaaggag gaaaaattta
    15481 tcgacggaat gggatgtatc gattggggtg aggtatttga cgagggggcg acggctaagc
    15541 aatattcccc tgtgcggcat tgtttcgcaa gctttggctc ggggaaggtc gaaggcggcg
    15601 ggtgacacac aatcagggta gatcgggagg gttccgatcg ggtgatgtta cccacactgt
    15661 aaccctagct ttgaggagga gtgagtcatg gctcggtacg gcgttgggat gctggcgttt
    15721 ctcgcgttct gcattttgcc gataggctca atctggggtc aatcggcgtg tcaacccgcg
    15781 tgtgcaccgg cctgtcagcc ggcttgccaa cccgcggtga cctgcgtcca aaagacgatc
    15841 atggtcccgc aatgggtggt cgaaatgcgt aaggttcgat gtatcgaacg ggttccggaa
    15901 gagcgggaaa tcacggtgaa cgtggtgaaa tgggtccccg aaaccaagat ggtcgagcgg
    15961 accaggacac aatgggtcct cgaggcacgc acgaaggaag tgaaatgcgt cacctaccag
    16021 ccggtctgga agaccgaggt ctgtgagtac atcgtgatgg tgcccaagat cgaaaaggtc
    16081 cagggagttc gcaaggtctg caagatggta cccgtgacgg aaacccgcaa agtctgcgtg
    16141 gacgagggcc actgggaaga gcggccttgc gagccggtct gcggcatttg ccgacattgc
    16201 caccgacggg tcagggtggt ccagtctgcg gcatgttgcc cgccagccaa cgcgtgtcag
    16261 ccggcctgtg ccgcacccaa gcccgtttgt gtctgcaaag tgtgggtacc aaatcctgtt
    16321 gtcaaggaaa tccaggttac ctgcatgaag ccccagtggg tggaggaacc ctgcgaatac
    16381 accaaatgca catgggtacc cgaaaagaga accagggaag tcaaacgctg cgtttgggag
    16441 cgacaggaat ccgtcaagca ggtgacctat agcgtatgtg ttcctcgtac ggtggtcgaa
    16501 aaagttcctg tcaccgtgtg caaaaaggtt tgtgagcctg ttgtgaagaa atgcacggtc
    16561 atggttcccc gttgcgtgga aaaggaagtc cccgtttgcg ttcgcaagat ggttcccacg
    16621 accatcacgg ttcccgttcg gccgtgcggt ttgcatcacc gcgttgccct gcggcgggca
    16681 gccccatgct gcgatccata agggaaaggc aaacgcttgt ttttgacgaa ggttttcgcg
    16741 caattgtgat cgccttatga tcgtcaaagg agcgtttgac ctagcgtggt cacccccgtc
    16801 ttagaaccga tgggcactgg cgccgactcg tgtgagtcgg cgttttttgt ttgttgtgcc
    16861 ccttcgccag gtatagattt cgtcggtggc tccgagacgg acaaaggctc tgcgagacac
    16921 atgaaacaca cagttgccaa gatcattttt caacgctggg ctacggacga aatatcctcg
    16981 acatcaaccg gcgtgatcaa ttcatcgtcc acgaatttga cgtcgtatgg gtttggcaca
    17041 tggaagtaga ttgcctcccc ggagggatcg ggaaactcgg ttagtcgcag agtgtaagcc
    17101 ggttgcgcct gggggtacca cgctccgggg gaccacgctt tgacctcaca cgccaatccg
    17161 ccaggtctta ctgagtcatc acgaaaaggg ccagtcaaac tctcagggac gattgtcagc
    17221 agccgcaaat ccacgtcttt caagtcaggg tggtcctttc gattgaggca aaacaccagg
    17281 ggaccataca tgatcgcgac acggcccact tgggcacgtc gccctttgac aaaccgaagc
    17341 cccatcggca gatttagggt gaccacatct cccggcttcc agactcgttt gagaaccgcc
    17401 cagtctccac ctttagcttc gagcgattcg cctcctggga ggtcgatcct ggcatgatca
    17461 cagaactttg gaatgcggag tcttgtttcg aaactctctt ccgcccccag atccagcgcg
    17521 atggtaacgc gaccatcgga gggatagtct gtcctctgga tgacggcgac ccgctgccca
    17581 ctcggaagat gaagcgtcac tcgtccctct gtgtagagat taaccgcaat cccctccgga
    17641 gtcgcatagg ccacaaaatt cggcaattcc gcgataatcc gccggtaatt gttgggacag
    17701 caatacgtat ccgacgggaa atacttccga ggaccatcaa aaggtgtata gtagcgaatt
    17761 gaccggccat cgggcgactg agcggcaaaa agaccgttgt aaatggcccg ctccattaag
    17821 tcgccataaa ggcttttctt ttcccgacgc aacagctcgt ctagccagcg gatcaaatac
    17881 gccgttgcac aggtctcccc caagttgatc gtaccggcct gcgtatcgtg ccagcactca
    17941 tggtctccgc attcaccggt aataacaagt ccttctttgt gcaggaggaa atccaaagcc
    18001 tggcgacttg cccgccaaag ccgctcctcg ggataaacag aactgagtcg aagttgggca
    18061 atactgcggc aaaggtaggc gtaaatgtgc ccctcgatgt caccatggcg cccaaccaca
    18121 atccgcgcat cccattcagg aagctgccgg aaattgacca caaaatcgcg gtaacgcgtt
    18181 tcgcccgttt cctccgccaa cagtaaaaag gcgttttcca atccagtaac agccatatag
    18241 accgttattg aaccaccgcc gggtttcgcc tccggatgag cttcccaatt tcggatgatg
    18301 taatctgcca aacgtttccc acattccaaa gatcgttgct ccttgaaaaa acgatggttc
    18361 gaggcgaggg catacacaag gtacgacatt tcgtggatgt cccacaactt ccacatgcgg
    18421 gactggggtt ccataatccc aaggtaacca tccggctcct gcaattggca caactcacca
    18481 acgatgcgat ccttcatctc gatcagacgg gcatcttttg tatgagcggc taatcgcaca
    18541 atgctatcga gcagctttcc caggccgaca tatccatcct tcttgttacg aactcggaac
    18601 gcctgtaaaa aatcccggtc cagatcgagt ttcagcagat tttcctcgat ggtgcagcga
    18661 attctccgcc cgatttcacc accaagctgc acctcgtgag gggccggcga ttcccaagct
    18721 tgtctgaggt cgctcgcgaa aagctcgccg aaccccgacg gcgcgcatcg catcggtagg
    18781 tccgccataa tgatcactaa acacaccaac gtcccaaaaa cgagtttacg acccatctcg
    18841 catctcctct cgtgagtcgc tccgccgagt ttcctgccat gccaattgtg caaaaatgtg
    18901 cgtgtcaact tggctgttat tatagcgtta aagatatccc tcggtcgggt agcccccggg
    18961 cgacaccggg aattgagaaa tgggcggttg aaaggacgat acgtaaaagg aagacgccgc
    19021 gaacaaattt ccatgctacc ccgttaagga accggatgat cccacggcct cgcgtcacca
    19081 caaagatgac cccgagatgg gttgttcggc cgtcagtagg ccgtgattcc ggtcgggtga
    19141 tttcaggaat tgcttcaagg actcgtcggg cgaaagagcc attgggtcac gtcggagtca
    19201 agcgagcgga agcgaagcgt tacaagatcg ctgcttgact gagcgattcc gttcccctgg
    19261 tcccttgcgc tagatagtac tccagacggg ccaacacggc atcccgatgt ggcccaaaag
    19321 gaacgaacac cgcccgcagg gcatcggctg gacagggatt gtgcgtatcg aacaggaaaa
    19381 cgccgtggtc ttcgatttga atggcccgga tggcccgcca cggaacccgt tgcaaacgcc
    19441 caaaaacaat cctctccacg ccatagggcc ccatgcgata agtcgcctta agaagggctc
    19501 tcgcaaacga ggcgatcaga agtatatcga caatggccgt ccacagaaac cgttcagtga
    19561 cccagaacac ggcaagagat accccggcca aggcgagcaa gaagacgatc aagaccgacc
    19621 agtcgtcgcg aatcggccag tcgtcccact ccaaaagggg cggggcctct tccatcaaag
    19681 aatcgtaata tcgcgcaata gtgctggcgg tactcatatc acctatttta acttggcttt
    19741 ggtgctaagt ctgccgcgat tttcgccgca atcaaacaga ttctgaatag gtccgcgggc
    19801 tgccccggct gcctacgccg gcggttcgac gtaacaatcg aggcattaca caccagcagg
    19861 ttacgccgcc agcctcacga tatatgtatt gctgttttct tgggtccact ttgtgcacaa
    19921 actgtggtaa cgctgccgtc acatcacccg ccgaccactc acgttagctg ctggaaactg
    19981 taaatgtgag ctagaccccg cttttctatg taagagccta tccccaaaag cccccctctc
    20041 cctccgggaa atggttgggg tgaggccaag cgaacgtcag aatcgattga gaaaaccggt
    20101 agcgattcga cccatgctca cccccgactc ctctcccacc aaacgcgtga ggggagatta
    20161 tgggataggc tctacatcaa aacgtgatca gaactggggt ttaccgtctt tcatggcctg
    20221 gtaggcctcg ggcgtgtcca ccgagatacc ggggtctttt tgctcggcca gccatcggtc
    20281 gagctcctca gacaaccgct ttttaatctc agctacttcc gggcgatcgg cgagattggt
    20341 catttcgtat gagtctgccg aggtgtcgta aagctcctcg gcgggacgga cacagtaccg
    20401 cttgaccaac cgatagacgt ccggccgatt ccaagagtgg gcgatccaag tcggccaata
    20461 agcgttgtgg gggacactcc cgcccagcat gcccatgagg tgtttctcga tgtacatctc
    20521 ctgtggtgtc aagtttcgaa tataatgcca gcggccgtca ctcacagaac ggattggata
    20581 gggagggcct tccgggaagt tattgtgcac gaaataggca tatgttcggt ggctatcttt
    20641 acggccaagg aggacctcca caaagcttgt gccgtcaaag tcacccatct ctttgggctg
    20701 gccccctgcc agttcaatca atgtcgggac cacgtcggca tattggacga tggctggggt
    20761 ccgtttcccc gccggaacac gccccggcca gcgtgcaatc aaggccgtgt ggacacctag
    20821 gtcccagttg gtccacttgc agccagggaa ctgcgcccct tgctccgatg tgaacaaaac
    20881 cagtgtgtgc tcgcggagcc cggcctcatc aaggactccc aggatttcac ccacctgccc
    20941 atccatgtag gtaatttcgg ccaagtagga ggcgaagtct tcccgcgtct tcggggtgtc
    21001 ggcaatatgg ggtggaagcc gtagcttctt gacgggatac cgcgaccgat cccccatcac
    21061 ccagggcacg tgcggctcaa ccaaggcgac caccaggcaa aatggctgat ccttttttcg
    21121 ggtcataaat tcgcggatac cggccaaatc gtggggcggt gtgggatccg ccgtacaatt
    21181 gggacaaaaa ccgggaacat tctcaaaagg aaatacctcc ttaggtgtta cgtgtacctt
    21241 accggcaagt cccacccgat aacccaaacg accaaggtaa tggggtaaac tctggatacc
    21301 agggcgggag gccgcatgat tccaggcaca accgttccgt atgggaaaca ggcccgtgta
    21361 aagctccgac cgacacggtt gacagatggc catcgccaga tacgctcggt caaatacgag
    21421 cccttccttc gccaggcgat caatgttggg tgtataagcg ttttcgcccc cgtaaacggg
    21481 caagtcattg taggtgcagt catctgccat gatgataagg acatttggac gatcggccgc
    21541 cactgccgac aactcgacta caccaaccgc caaaatacct agaagtaaca gagccgctcg
    21601 catagctacc tcccacatcc gcaattcaga atgaggcacc aaaagcctga gactcataat
    21661 aggctaacta gacggctccc ggaagtacat cgagcctagc agcttgagtg aaaaaaccca
    21721 acgccgccac caatccattt gcgtccaaac cggttgaatc agataattct actcgatggc
    21781 ttcaaactcc catgcctggg cagagcaagg gggcagggtc atttccacag cccatgtgct
    21841 gggccccact tccgtttgct gccgaccttc gggaagcaca caggtcgtgc gaattttaga
    21901 agcattcaat ccccactggc gcgtatcggc ttccacgcgg accgtcaatt cctcctccgc
    21961 ggcattcacc atgaaggcga tcgcctttct ttcccgagga attgcccatg cacctgtgaa
    22021 cactgcgtcc gtggtcaccc acatagagcc ttgccattgc caatcagcgc gaagctgagg
    22081 aatgttcccg gcaacgcgag gtggccgaac catctccccg gcataaaaga accgcaccgc
    22141 ttgcgagcgg agcaaaacga gcttcttaag gaacgaggca ttctgcggct cgtccaccac
    22201 cgcggggtta atccaaccga gctgttcccc aaagacaagc tgttgcgacg cccgcatgcg
    22261 gagggcaatg tcgcgatttc gtccggcggg ttccgtcggc cgcaggccac tccccgttcc
    22321 accgtagtat cgcccaaaca tttggatggt gcctccatac acggcaggaa acagtggaac
    22381 ctggttcgga tattgccagt gccaactgag gtagccgtca aaccaacgga tgtacggctc
    22441 ggcattacac tcggtggtaa tcatccgttc ggacggcatc gccttgcgaa tgccctcgag
    22501 caattgccag taggaagtcg tccaccaatg accgcccccc aggggatgac cgtggcttgc
    22561 atcgaagcag agtgttggcg aggccgcggc aatttggtca atatacacac cgtggacgcc
    22621 acactcggag aacagtcggg cgacaattcc ctggacggtc tcttgccaaa gggacgtgga
    22681 aggacacatc acacccaagg acaccggact gccatcgctc tctttgctgc tgtattgttc
    22741 ccgataaggc tcaccaactt catttttgga aactgccggt tttgcacgcg aagagaactc
    22801 aaaatcttcg agcccccggt cacgtgtatc ccagaggcga ccattgatgt aaggcatcac
    22861 gtgcaccccg ctcttttgaa gttccgccac ggcctcggca aagccctcct tcgtgggaaa
    22921 gtaatgggga tagtcattat caaatgggat ttgatgccaa ttgtaccagt gaaaaccgca
    22981 cggcacgcca aaatactcgg cgaatcgctt cacgcgttcc acgcagtccg ccggggcccc
    23041 tccaccaagc gcccataggc aaagttctcg catccaaagg gctgtgtctt cccgaccatc
    23101 ggcacccagt ttggggtacc agcgtgcttc gcgccgcacc cagtcgcggt aaatcaaggc
    23161 catgtcaaac caatcaccgg caaaagcctg aaataccgcc tcgccgggta actcgaagtg
    23221 gttgcccgct tggcccatgt tctccgcggg gtgctccact gcccaggtaa caatatggtc
    23281 ctccggccgg ctctggagtt gaatttcctt tgtgcttccc cagggatcgt ggaccgccag
    23341 gtaaaggcct gtgctgttat cagccgcata agcccccacg aactgcatgg tcacccagcc
    23401 actgggatag cgaccgcgaa actggagtgc ccgctcccac gccccctgtt cagcgatgcc
    23461 cacgccttta ggataaacca cccaggcatc gcgccctggc tcggcaatgg caacctgggg
    23521 gaacaccacg gaccaaatgc tccactcggt gctctcgttt tcgactttta gccgccagcg
    23581 gatagcctgg cgcgattctt cagcaatcgc ctcgacctcg acggcgatcc ctttgaggcg
    23641 cgcatcactg aagtcctgcc agcggacctt caacgaagaa tgcccgtccc ctagttgaca
    23701 ggtaacctta ttccagccag cgtcggccga caacacgagc tcttccttgc tgacagcatg
    23761 tctcgcccga atgcaaaaca agggcaggtc ttctctgacg aacaaccgat cctgacgaac
    23821 atcataagcc accgggcctt tgagaccgtt cggaagtttt tccataacca gcgaaagatt
    23881 gccggccgcg atgcccgtca aaaactcggg catcgtacct tccgcagcgc gcagggcatc
    23941 ttcccagtgt ccggttcgct cgcaataact cgtcgccgcc aagaggagag gcgaaagttt
    24001 tgcccgcgcc gcggcaaggc ggtcatgaat ctcacgagtt gttacgatgg catccacctg
    24061 cttggggaca aggcctggta cctcgcgaat ccgcccatca aagtcccctt gctcccccac
    24121 aaagatataa gcctccttgg tgattttggc ggtctcccac ggttgccacg ccctatcgcc
    24181 atgggcttga atgtaatgcc cgtccccgcc gggcgagtca tagaccaaca cgctcccacc
    24241 gcaaaagatg cccaatccta cctgctcatt ccgaaccatg ccaatatggg tcagcggatg
    24301 cgacttcgcg gtccgcgtga gcgtaccttc ttgaagaggt tcaccgccca tccaccggtt
    24361 cgccgtgttt tcggaaaact gaatctccaa gaagtggtgt tcgttccagc gaaagccctc
    24421 cgcctgggtt gcaacgacac gtacccagat cacgggaaga tcgtagaaat agaaccaatc
    24481 gtaacaggct tgaggctgcg aggttggaga ttgctcccca cgcacatacc gcgtttgagt
    24541 gcggacatgg acgccgagag gaccgcggct cagcagtttg acctgggcgg aagaataacc
    24601 cgctgccgaa aagcttccct cctttgtgtg atggacgcga tcagcaaatc gcacgggaaa
    24661 ttctaacgca gattgaccaa tccacacttt ttgaaagaac ccaccatacg cggcattatg
    24721 ctcgaccgaa agataaggca tcgacacttt ctcgtcgaat gcctgggccg agtctgagtc
    24781 gctatctgct gtctgctcag agcggaggac aagccttgtg ggtggtggtt ccggcagtcg
    24841 aaggataagt atgcccgcac cggaaaattc cgcaccggtg gccgatgtgt ttgacctggt
    24901 gaacggctcg ggtatgaatt gggcggggac cagcacattg tcttccgtaa acaccctgag
    24961 gttctgccaa gccacgcgag acaacccaca ccgttttaag acaggtccca ggtcaacgtt
    25021 aacgcacacc attccaccgg aagggacatc ctccaaaacc aagagcggtt catctcccag
    25081 agcgaagatg gcactgccaa gagccaggaa cgtgccgaag cacacaccac gtgttaactc
    25141 gcgcatgggc caaccctttc cttatcactt ccgacgtggc tgagtattcg ccgcggccct
    25201 gatcggtggg ggcgaatgcc aagatatgtc atcattcggg cgctatcaag caccagagga
    25261 gtattgaaaa ccaacaggga aggtccttgt tcgcgatcgc gccgctcatg gtttgcccgg
    25321 tgccatttcc acatgatcgt gcactgcaac aacttcccca gcgtagtttt cacggaccga
    25381 tgtaattgtt gtagcctcat ccacttcgcc cgggacaatg tgcgtgagga cgagcttctt
    25441 ggcacccgat tgctgcgcta tggagcagac ctcttggaaa ctagggtggc cccggacggc
    25501 gcaatgacgc ggtcgcttcg ggacttgccc acggaaatgt acagccggct gcttcggctg
    25561 ggcctgacat caccacggaa aaaaccatat cccgaacctg gagcaatttc tccgacagta
    25621 cgcgatcgcc cgacccgacg tcaaagccgc caccaaactc gcatggcagg cccttgtgcc
    25681 tgcccaaggt ccccacgccc agcgtaggcc cccgtgcgta gccgatgccc aggtggttgt
    25741 tcaacaggag ggcaaccaca gggggttgcc cctacacgca ccgcatgaaa atcgtagggg
    25801 caggcccccg tgcctgcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    25921 ttgcccctac acgcaccgca tgaaaatcgt aggggcaggc ccccgtgcct gccctatgcc
    25981 ttttgcgtgt cccagtcccc tgtgcctgcc ctagaacgtg aattgcgccc ggggcgccgt
    26041 cgaatccaaa cgacgggtgc ccgccctaac gcgaaccgtg gggactgcgc ataaaagggg
    26101 caaccagggg ggcacaatcc agggcaacca cggggggttg cccctacaca acccaccgaa
    26161 tccgtagggg ggaggccccc atgcctggac tgtccccagg ccttccacga tctgcgggca
    26221 agcgtgacca cggacctaac ggcaagtttt ccttccccgt gatggccacc tggcttgggc
    26281 ataccgaagc cattgccgaa gcgcactact ggcaaacgac cgaagcccac tagcgatcag
    26341 ccccagactg ggagccaacc ccccaaatgc ctatcccata atctccgttc acccgttcgg
    26401 cgggagaagg ctcaggggtg aagatagctc gaatcgctgc cggtttgctc gatcgactcc
    26461 gacgttcgct tgccgtcacc ccaactctct cccggaggaa tagggggact ttttgggaca
    26521 ggctccctgg gatattctcc tgagccctgt tgcacccact gctgaagtaa actgcagtgg
    26581 tgagggaaat cgtcacgaaa gtggcgaaat acttccccga actataaccc ctgccgtacc
    26641 ttgcaaggta aatcacttct tggcaatgac ttagggataa acggcgtttg atacgaactg
    26701 gacaggattc taaacagccc ggagcctcgc tcattgaacc tggttatttc gggacgcggc
    26761 cgggcttgtc cacacagtgg tcacaattat aatggcaaat ggagatagtc tggcttgctc
    26821 acaggatggt taggccgctt tatccacctg tgatgaggga cgcatatcgg ccaggtagca
    26881 atgatcatta ttatcacgaa ggcgtcccac cgagacgacc gcttggtcga tcctaatatt
    26941 cggtgtttcg tgtggatggt gtgggttgtc agagagagga gctgaaagga cgctagaaag
    27001 aggtcgttgc ccgaagcgtg gaagacagcg taacccgtta tagtcctcac aattatattt
    27061 tgtgctcaac ggaaacctca tctcgggcag accttggaca ggcgcgcagg gttatgatgt
    27121 atgaccttcc tcctgtcagc atagttggga cgtcctttct gcgattgtct ctgaggtggc
    27181 aaggatcacc caggtgacgt ctggcaaccc agccgggatt tgaggagtcg ctcgtcaaga
    27241 ttcatggctt ctcattggcg ctacatgagc gaccgattcc tcttccggtc tttggcggtc
    27301 ttttgccctg tctttctggg gctaaaggag ggagaccatg cgcgtctcgg atttgggttt
    27361 gccggccact gctattgccc cggtggtcac gcctaacaac ggcaaaacga accaagggca
    27421 acagaccagc gagggtgcga ccagccaacg tttacaaccc cctcaaggag accaagcgcc
    27481 gaagcctctc caccggttgg cacaagtgcg gatacgtcag ggggtttctc gtcgcaccat
    27541 ggcccgtcgg atgaaggtcg atatccgcac cgtccgagaa gaagaggatc cgacgcgaga
    27601 ccttcccctt tcggcactat atcgctggca aagggccttg caggttcccc tgattgaact
    27661 gctttccgac atcgaggaac cgctgtcggc gccggtcctc aaacgtgccc gtttgctgcg
    27721 aatgatgaag acggtgcggg cgattcagct tcgggcaaaa cagcctgtcg tgcggcaatt
    27781 ggccgaactt ctggcagaac aattacttga aatcatgccc gaactggccg atgtcagccc
    27841 gtggccaatg ggccgccgtc cacgaaccag cgactgtggc cccacggcgg atcgcatttt
    27901 caaggtccgg tttcggtaat cggcaattag cagcttgata cggctcgcgg tgtgcccaag
    27961 ctttggggaa agcgctgtgt cgctttagag cctatcccgt aatctgccct cgctcgtccg
    28021 gcgggagagg ggtcgggggt gagggtagct cgaatcgctg ccggttttct cga
//
LOCUS       DAOSUT010000035        28025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1117_length_28025_cov_9.574687, whole genome shotgun sequence.
ACCESSION   DAOSUT010000035 DAOSUT010000000
VERSION     DAOSUT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 28025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28025
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1117_length_28025_cov_9.574687"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(633..1982)
                     /locus_tag="PLD05_04840"
     CDS             complement(633..1982)
                     /locus_tag="PLD05_04840"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76793.1"
                     /translation="MAAKRPKVSRRVFLKWSGAALPLIPTFVPSHVFGQTAPSEAVCL
                     GIIGTGRQTFNVNIPQFQNQPDCRIVALCDVDRWRLEQAKARIDEFARKQYQITTGAA
                     TYVDYHDLLAREDIDAVLIATPDHWHGVMAVDAMRAGKDVALEKPIIRTFSEGKRLAE
                     TAARLNRIFRVDSEFRVGAPARRAYSVLRENLLGKILRVKVGVPQTDVACPPQPEMPV
                     PEELDYARWQGAGVTQAPPIPYTLNGVHPRHDLKGRPGWMRRLNYCDGMITNWGTHLL
                     NGALWCLGLDRSWPTEIEGTGIYPPKDSFWNVLLQFRVIYRFENGLEIEYHTERPYMR
                     FEGEKGWIEADFSKFQASDDALVKKSFPLTDSPHPRFTSEKRDFLDSVKSREPTWEPA
                     DVGHHVTSICLMGHIVVFLGEKLRWDPDRESFVDNPEANKYLSEPIVAPNPRFQKPA"
     gene            complement(2051..2932)
                     /locus_tag="PLD05_04845"
     CDS             complement(2051..2932)
                     /locus_tag="PLD05_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP76794.1"
                     /translation="MMSLRMIAYAKPLSSLIVATSLLSSASQHILAAEPEFGVLTTTQ
                     EMRQMPPAVLAALLRETGYQSVGAICLAQQLPEWVETFRRKDLRVGNVYIKTIVKPDA
                     CEFECPLDDVFKALEGSNAVVMLNIHEAGTPVDNAVIAQHLRPWAEKAAKAGVQLAIY
                     PHVGFRVAKDEDALAIAKTVNHPHFGVCFNLCHFLKQHEMRELRPTLEKGLPYLKMVT
                     INGSAIGDTQNMNWDKLIQPLDEGEFDLSAFLHTLYCELGYRGPCYVQCYGLTVPAKE
                     LLERTLTAWKQIMPRCP"
     assembly_gap    3281..3380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3450..3989
                     /locus_tag="PLD05_04850"
     CDS             3450..3989
                     /locus_tag="PLD05_04850"
                     /inference="COORDINATES: protein motif:HMM:NF022061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76795.1"
                     /translation="MKLGYLCLVSVISVALVSGFAWAEPGKKAKKGEKAPPTPEELFA
                     QMDTNNDKAVNLDELLAMKQVKGDKEFAQKLLALWDENKDGKATKDEFNKFLESQAQQ
                     REKQFAKVDLNADKSLTLDEIKSMKQVGGDETKAKAFLERLDDDKDGKVSLKEFTRVR
                     AIFQLPPQEKEKKEKKGAN"
     gene            complement(4214..4789)
                     /locus_tag="PLD05_04855"
     CDS             complement(4214..4789)
                     /locus_tag="PLD05_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76796.1"
                     /translation="MIHVCWLPLFVLSTMLYGPSPGTFSTVVQRRLMVVPYSEVCKVE
                     MRLPGAYCHRVFETHMVPYFAQFPIVEIDRSSAQFWASMLYRSDFARYGDQWDWRARL
                     SYATYLAGLRNGLIVPKPQLHPAGAGSRTECPPGERPHPVVVLNLFCGEKTEGTMDRV
                     IAPPLLEPKPTVRITTSSAVLPMRVTNPFIP"
     gene            complement(4940..5302)
                     /locus_tag="PLD05_04860"
     CDS             complement(4940..5302)
                     /locus_tag="PLD05_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76797.1"
                     /translation="MQKVLVQLWSQEDGVLTFEWILLITILVIGVVGGLSAVRDAVIT
                     ELGDVVEAVISLDQSYLLLDPWEIRVPDCEADGASGSSYQDSAGMSQERLGNCNQGCF
                     LEQDQDLGDCSPYIERPF"
     gene            5883..7640
                     /locus_tag="PLD05_04865"
     CDS             5883..7640
                     /locus_tag="PLD05_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOP76798.1"
                     /translation="MNKLTNLLLEKEILLESGTNELEILVFDVGQYVFGVNVAKVREV
                     LPAPTITHLPNAHPSIRGVFKLRNQVVPCVSLADYLGQYDGGDDRESVCILTDLNQQQ
                     TAFLVDRVERIHRLSWENVLAVPPLEDLAQTPVTALARCQDRLILMLDFEMILDQVTE
                     HYFRTESVENPASLPREQAVILLVEDSPTVREAITQTLSASGYSRLVVFENGKHAWEW
                     LEKKVNDQSSHGCLCDLVIADIEMPQVDGLHLTKRIKSHPQLKDIPVLLYSSIITPEN
                     RRKGEAVGADAQVAKPDLCHVVEYADNLIFGRRDNSGSHFSNVKDVSNVERTPAVANP
                     TSMPQAETHSNDEQCVSAGLAEEPTSMAEPQVAGSIEPEANVERPSGAGDKNCLSGHY
                     DQCVVSPAVTDEHESMDNCSRQVEVVASESSESEVSDADDDEAIIGLRPDVVENERLW
                     NTFACELQHRWQALSELRRQISAKGETFEPAKDFDIVVDVARILHTIKSAAMVVPIEI
                     VTRTTHALESLIEAGSDRNEWPAQAFNRYCDWLEMFLNPVDQTWDSWRETGNYLAADL
                     EQQLAQPAEAPQTTTSCQG"
     gene            complement(7800..8606)
                     /locus_tag="PLD05_04870"
     CDS             complement(7800..8606)
                     /locus_tag="PLD05_04870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76799.1"
                     /translation="MPVAQGTWTTVFRATVASNPPNRPPRYAIKTLRTDLTDTAIARG
                     HLSREAEVGRTVVHPHVVPILAWRLTGDWPYVVMPWLEGTSLQQLLSQRGAIPTADAI
                     WFVRQIAEGLAALHGAGWLHADLKPSNIMVNPEGHVTIIDFGFAQRWEEGSLSPREVV
                     GTLDYMAPECIFRHAPWGPKSDLYSLGVIFFEVLTGQRPYCVRSLREAARVHRQRRVL
                     DVRILNPSVSHEVSRLIRQMMSPIPERRPASAAAVAEHLVRLEIEHFTFR"
     gene            9061..9783
                     /locus_tag="PLD05_04875"
     CDS             9061..9783
                     /locus_tag="PLD05_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76800.1"
                     /translation="MHTDDTTAPFVQMRNVSKLFVMGDVVVEALRDITLDVYHREFLV
                     VLGPSGSGKSTLLNLLGGLDTPTEGQVLFEGRDLAEFDRRELTWYRREIVGFVFQFYN
                     LVANLTALENIMLAADLVSDPLDPVDLLEQVGLADRADSFPAQLSGGEQQRVAIARAM
                     VKNPPLILCDEPTGALDDMTGRQILRLLLNLKEDMGKTVVVITHNTAIAQMADRVIRL
                     RSGRIVEVTEQASPLPPEKIQW"
     gene            9831..12209
                     /locus_tag="PLD05_04880"
     CDS             9831..12209
                     /locus_tag="PLD05_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76801.1"
                     /translation="MNPLHRKLIRELRASGGVIFSVILITAIGTACFVAMRSAHRSLS
                     QARDHYYAKGRMADFWVSVKRMPKSELRRLSNIPGVAEVHPRIRSPVTVDLPTSEKPL
                     AGVVLSLPDRGLPALNQIQILRGEYFTSRRRNEVIVNDVFANAHRLRLGQSIFVNIAG
                     RRQELVLVGTAFSCEFIYPLGRAAIVPDRTGFGVFFLKESFAEEITDMKGATNEVLGR
                     VFPRYANSKRALLHEIERRLDEFGVLATTPLEDYPSNAFLSQEIEGLATFALLIPLIF
                     LGISAVILNVVMTRRVEQQRIAIGTLKALGYGDAVIGWHIMEFALLAGVIGAAIGCGV
                     GQWLSAGMTSIYRQFYEFPRLDAPIYWDIHLEAILVGLICAAIGGWRGARTALALPPA
                     AAMRPRVPASGRHILLERFGWLWRHVHPAWRMALRYMFRHPIRTATSLLACLLGASVM
                     ALIFCMIVGVNHLMDFHFRWLYRSDVTLFLKSERGSDALMEVAHLPGVRKVEPLLGMP
                     VTLRHEGKEKLISVTGIQRTATLTLPRDRQGHHITIPPTGFLMSRQLAKMMGVKPGEY
                     IDWQPMRGDRRWYRTQVVNLMDDYVGTAVYADLTFLRKCLGEGSALNSLQLAIDAGHE
                     QRRAFVKKIRDWPEIEAMIYRADMENNLRKTLLQNIWVILSIEISFAGALFFGAILNG
                     AIISLEERRRELATFYVMGYTPWQIGGLLGRESVVVVIGGLILGIPVGYFFARLVVAS
                     YQSEVLRMPLLFPPQVWIQTAIWGCLFSLVSHFVVQIMIHRSRWAEAVKVAE"
     gene            12479..13669
                     /locus_tag="PLD05_04885"
     CDS             12479..13669
                     /locus_tag="PLD05_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOP76802.1"
                     /translation="MTRLRDTVLLTMPFSGRIEAIPWIEGTEVKKGQVLARIVPSDVE
                     QMLEEARAVVARIEAAIRTQKDNRVEETAIAQAQAYVESMKSTVAAAAARVEASEARY
                     NYAQKYAARIAGLVQSGGASQDDLDRTELLKIESALSYQQDRLVHAALVSLQAATNLL
                     PVLIQKYVERKALVEDELVQQLAEAKARLAMVEENARRATMVSPYDGIVLHRYVSQER
                     FLAAGTQLLELGRWHDLEIETEILTQDAVRIKQGMLVDVFLGEDLARPVCRGKVSRIH
                     PAAFTKLSSLGVEEQRVKVTVELRPEDIDLLRRDYNVGVGYRVWVRVILQEKPNVLLV
                     PRISVVREETDRWVVWVWNKRRVERRVVTVGIMNDSFAEILSGLAEGEEVVLTPETGG
                     AASH"
     gene            13792..14622
                     /locus_tag="PLD05_04890"
     CDS             13792..14622
                     /locus_tag="PLD05_04890"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOP76803.1"
                     /translation="MGIRFYCPNGHKLHVKAFQAGMRGICPYCGAKVQIPLHSTRPST
                     KELKARRAAGGPEHAAIPRGDTAMPESRQPGVDSSPEKAPDGQGVLPGEQEQLTPFID
                     LDKVLAARNLGDQGGLRSSPSPLEGDLAISTAESVRSRNSVTEEWGSPGESARSFEKD
                     PLAEPNVVWYVRPPSGGQYGPAAPEVVRTWIAENRITPDTYVWREGWRDWQLARDVFP
                     EVLAKALPEVPVEDQPERRLIRRSVRSSWAQYAIVAFAAIVICVLGGLVWLFVSRSSP
                     "
     gene            14783..15676
                     /locus_tag="PLD05_04895"
     CDS             14783..15676
                     /locus_tag="PLD05_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP76804.1"
                     /translation="MTDAPKQNRRGFLVSSGITAATLMMAPSVVPTTAQAKEASTPRA
                     PRFRLALASYTLRLFPLDQALAMTKRVGLKHICLKSFHLPLDASADQIEAAKKQVAEA
                     GIDLYAGGVINMATEAEVDQAFQYAQLAGMRVIVASPRPPVLERVNAKVKETGIAVAI
                     HNHGPGDKFFPTPESVIEQVKNLDKRIGLCMDVGHTARIGADPIQDARRFADRLLDVH
                     IKDINVAAPEGRCVECGRGVLDLPKFLKVLIEIDYAGMVAFEYEKDEKDPLPGLAESV
                     GYVRGVLDSLLGISDRSAQLE"
     gene            complement(15742..16449)
                     /locus_tag="PLD05_04900"
     CDS             complement(15742..16449)
                     /locus_tag="PLD05_04900"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOP76805.1"
                     /translation="MGDPEILYEDNHLLAVNKPAGLATMGLPEDQETLLSWLKSWLKR
                     RDAKPGNVYLGVVSRLDVPVSGVVLIAKTSKAAARLTEQFRTRSVSKIYWAIVEGRVP
                     QPAGTLRHFLRSDERFRRVHVTHAHDPDGQEAVLSYKQLAASRGLTLLEVHPHTGRKH
                     QIRIQLAVMGCPILGDQKYGAKQAFAEGIALHSRRIEFTHPVRKESMAIEAPLPVSWA
                     KLPFAWSEISSRSFRHE"
     gene            complement(16637..17227)
                     /gene="hisB"
                     /locus_tag="PLD05_04905"
     CDS             complement(16637..17227)
                     /gene="hisB"
                     /locus_tag="PLD05_04905"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOP76806.1"
                     /translation="MTPRTSQIERKTLETEIAASINLDGKGESRIDTGIGFFDHMLTL
                     FAKHSCIDLEIRCRGDLHVDQHHTVEDTGIVLGTCFRNALGDKKEIRRYGHWTLPMDE
                     VLATAAVDFGGRAYFVFNAQFSRETIGNFETELIREFWQAFAHCAEANLHICVHYGTN
                     NHHIAEAIFKATARAIRMAIERDPRGEGIPSTKGVL"
     gene            complement(17243..18280)
                     /gene="hisC"
                     /locus_tag="PLD05_04910"
     CDS             complement(17243..18280)
                     /gene="hisC"
                     /locus_tag="PLD05_04910"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOP76807.1"
                     /translation="MRPNILKMEGYVPGEQPQEDGYIKLNTNENPYPPSPRVAETITE
                     VLRRGLQKYPDPLATKFRKTAAAVLGVDPDWILAGNGSDDVLTIVTRAFVGEGDQIRY
                     PYPSYLLYSVLAQLQNADSEFVLFEKDWSLGSSFWKEKERLKLIYLANPNSPSGTMIS
                     KDVIKREIEHLRCPILIDEAYVDFAEESCLDLVREYEHVMISRTLSKSYALAGLRFGF
                     LIAQPHIIRELRKVKDSYNCDALAIAGATAALSDQEWLQETRRKILATRERLMRELRG
                     LGFDVLPSQANFVWCSHPERASKEIYERLKARKVLVRYMNYPGWGDGLRITVGTDAEV
                     DGLLNSLREIL"
     gene            complement(18349..19695)
                     /gene="hisD"
                     /locus_tag="PLD05_04915"
     CDS             complement(18349..19695)
                     /gene="hisD"
                     /locus_tag="PLD05_04915"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOP76808.1"
                     /translation="MLNIRWVDCRTEQWRKVFDELRARLSLRGDIVTPESQEKTVKIF
                     GEPLTPAQVVERICHDIRTRGIEAAIEYTRKIDAVELTPETVRVPVDRIKAAHEQADP
                     AFLQCIRRIRSNIEEFQRAILHHDVRVTRPGGYLLERYLPLTRVGVCVPGGAAAYPST
                     VLMTVIPALVAGVREIAVIAPPTRFGAFNPDILATCAELGVWEVYSLGGAQGVAALAY
                     GTEVTPRVDKIVGPGNLFVALAKKYVFGEVDIDSIAGPSEVAIIVDDTANPTFIAFDL
                     IAQSEHSPGVSVLIGWDEDVLRRIVMTVEECLEKVDRGPLARRCLEDFGAVVLVKDQD
                     QACQVADELAVEHLQISTRNAEALAQRIPHAGAIFLGHYTPVALGDYAAGPSHVLPTG
                     GTARFASALSANDFLRATSILYFDQSGLEHLADDVAYLATKEGLTAHRESVAVRRG"
     gene            20122..>21402
                     /locus_tag="PLD05_04920"
     CDS             20122..>21402
                     /locus_tag="PLD05_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76809.1"
                     /translation="MSAEFVIQCPTCLARLRVGSAKAAGAILPCPKCGSMVAIPLLAG
                     IPNGSGEELSGLGKLTEVQEKEEAKQTAGDSGLVEAEQAADRSNEKAVGISDQKRQES
                     IAEQGKDDSAEKPQTTGKAECENIAECSAESYAYKIRRIVFAVSALCVLVLAVWFWWI
                     GHGGNDVPEPPDIQKTEEIAKGDLTTPTAVEQQEEGHASWVPSGADFFIYEDTSADLT
                     GHDRGVVANVFALSEASARADQLLSVLGLKREVVAGRFISADARFATTVVVFQLKQDQ
                     RAEQLNVLGEPWENVGPVQLRQLAVGPERFAFAVVAENCVAIGQPEILKRLLSGDHDP
                     NIKAILEQKLKSKPRYLMVIRPTLIRHRGMMAWFDAWPEIDQLWQNLWAGANLFIFSI
                     ERSEQQVIWTCEWIAKSPDDKDSLEKSVRGLLTRV"
     assembly_gap    21403..21502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21503..23398
                     /locus_tag="PLD05_04925"
     CDS             <21503..23398
                     /locus_tag="PLD05_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76810.1"
                     /translation="VMGEWRHWAEIAMTRRHGHLGKAMSEYESKQQMFPMAAGGGVLL
                     PPETRLSWIADLLPYLGREDWAKALQKGYSWNSAQNQEVTQRYLPDVTNPLLGPTQST
                     AGYFDSHVVGVTGVGEDSGELPPSHPRAGVFNYRHPVRRSDITDGLSNTIAMLGVENK
                     RGPWAAGGLSTARGLTKPPYVNGSDGFGSAMPDGMLATMADGSVRFVRKDVDPRVLEQ
                     LATIAGGEPVTVDLLGSRASTAKGEAAPSGKNGTSGPVGVAAARLPYIPTMGTWRDPC
                     GSSLETASDNDGFSDTSWRRRVAQVRFRASLALAISLLTQWSRVPVCVDPESAEWLRL
                     TGNEEIQVEMQNVTFEDITRAMGEEVGLSLVPCKQSVVLVAADRIRDHVRYEFQMEEG
                     ESGTFPSSTTLEQVCDWFNEVSPGGFDKPAQVAKEGDRLVIDGPFPVVFEMRRMLEHR
                     AAMGSTNERLDAEDEATISLNFFEPTRLTDIAVAINRTAANKVFFDWTSLARIDVTPD
                     TEVSISATDEPLSGVLERLAELIQAQVHAWDDGPFVVCAPNRLTSGVLRIYYVGDILE
                     KGTTPGQLREQLIRECAPESWADGGGTGMLIYDAPAQAFVVLNSAIVHREISSWLQKR
                     REGQQGQ"
     gene            23469..23957
                     /locus_tag="PLD05_04930"
     CDS             23469..23957
                     /locus_tag="PLD05_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOP76811.1"
                     /translation="MDRRRKFLRRFSKRSLLAVVKRYRDAVRNEPAVHRTAAWWLAYY
                     QRSVAELLLIIWAGMGLFVGFRQLGDGLSLWFNTSSPRSYAFIVEVNSADWPELTCLP
                     GIGEILAKRIVMTRQEMGSFSRSEDLRTVKGIGPKTVRKIAPFLRFNNDRPIERLPAT
                     RK"
     gene            24072..24704
                     /locus_tag="PLD05_04935"
     CDS             24072..24704
                     /locus_tag="PLD05_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOP76812.1"
                     /translation="MAKVLITSGPTREYLDPVRYLSNASSGRMGRALAEVFLEHGHQV
                     FIVSGPVEIEYPNGADVVPVITTEEMLQECQRRFEQCDGLIGAAAPCDYRPMTFSPEK
                     LRKSGHTLAVELVETPDILATLATRKGSRWIVGFALETHDVHNRAMEKLRKKQCDLIV
                     INRPAAIGAQTTEVEIADANGVILGNWTGSKEAIARRLYEIVAQRFLRRG"
     gene            24802..25764
                     /locus_tag="PLD05_04940"
     CDS             24802..25764
                     /locus_tag="PLD05_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOP76813.1"
                     /translation="MADRHDVVSAGILFADVACEPIERLPRAGELIPTKRIQLGLGGC
                     AANVAVDLKKLGISVGVSGCVGDDFFGQFISQVLDREGVDTGGIRSVPGMTTAATMMV
                     NVKGEDRRFISTPGANVVFKAEDIPTAWTHAARVFYVGGYLMMPGLETDDFVYLLRDL
                     RAKGVITVVDVVLMERRDFWPILRKVLPEVDYFLPNDDEGEIITGVADPLEQAQRFHE
                     AGAKNVVITLGEKGCLFYGEGAKFTAGVYPTTFVSGAGAGDAFDAGFIAGLLWGGSPE
                     DCVRWGSALGASCVRAIGTTEGVFTREEAVAFMASHELPISKLK"
     gene            25824..26813
                     /locus_tag="PLD05_04945"
     CDS             25824..26813
                     /locus_tag="PLD05_04945"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOP76814.1"
                     /translation="MRIGVLTSGGDAPGMNACIRAVVRAAISMGHEVVGIYRGYAGLL
                     REEFYKDERGHPMMNLRSVSDISRRGGTILHTSRCKEFYTDDGLRKAAEVLRRNKIDA
                     LIPIGGDGTFRGAVALAKYWDGQIVGCPGTIDNDLIGSDYTIGFATAVHTAVEAVDKL
                     RDTADAHERMFLVEVMGRHSGYIALYTAIAAAAEIACIPETPTDTGEIVRLLHDLKLR
                     GKTSVMIIVAEGDERGGAAVLQKALCEVKCPYEVRVLALGHLQRGGSPVPQDRILASR
                     LGDFAVHAILQGKSGVMAGEQKGELVLTPLEETFAAHRPVPEAYVKLLETLAT"
     gene            complement(27103..>28025)
                     /locus_tag="PLD05_04950"
     CDS             complement(27103..>28025)
                     /locus_tag="PLD05_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOP76815.1"
                     /translation="PYGVRYWGIGNEMYGNWQLGHMPLEQYVEKHNAFVRAMREKDPH
                     IKVVAVGNVGPWTEGMFRHCADFIDLMSEHFYVGAKKDLIEHVNQAAAQVRRIADAHR
                     RYRRDILGGREIPVALDEWNYWYGEHVFGELGTRYFVRDGLGIAKALNEFARNTDVYA
                     MANYAQTVNVIGAIKTTKTAAAFETTGLMLVLYRRYFGQIPVATASQNPLLDAQAALL
                     KDNKTLAVAIVNPTGEKQLCTLRVAGATVGGSGKVWDVAGNDPMDYNDPGKPPRIELR
                     SRGEVSHADSFEVAPYSATIVQFPIVRAGN"
BASE COUNT         6296 a         7191 c         8011 g         6327 t
ORIGIN      
        1 gtcgattccg acgttcgctt gccctcaccc cagccctctc ccagagggag agggggactt
       61 tttgggatag actctacggc ggaatcgctc tgcggatctc gtcgccgacc gaggcccgac
      121 gtacccaagg ggccaaaata aaaaaacact ccacctcaga ccgggcctgg tatttcaggc
      181 caagcggtcc aagacggagt gctcttttct aggctgacag tgccgctacg gatgccagcg
      241 agctactaaa ttgtcattcg gtgcgggccg cacaaactcg ttccccccgc tagaaccgaa
      301 aatctaaaag cgcccatcga agggggaaac ttgccgcacc gattgcctat attttaagaa
      361 gctcacctct gctttcaaga ccgggatgca aaagtggcaa gacctcattc ttcaaggctt
      421 ggacctctgg ccgagaaccg cttaagagcc tatcccataa tccctgctcg cctgttgggc
      481 gggagaaaag tcgggggtga gggtggctcg aaccgctgcc ggttttctca atcgattcga
      541 cgttcgcttg ccctcacccc aaccctctcc cggagggaga gggggagttt ttgggataga
      601 ctctcatccc ccagtccgta gatcccgaac cgtcacgcgg gtttttggaa acgtggattt
      661 ggtgctacga tcggctctga cagatacttg ttggcctctg gattgtccac gaatgattcg
      721 cggtccgggt cccagcgaag cttttcgccg agaaagacga caatatgacc catcagacat
      781 attgacgtca catgatggcc gacatcggct ggctcccacg ttggttcacg acttttaaca
      841 gaatccagga agtcgcgttt ttcgctagtg aatcggggat gtggtgaatc ggtcaaggga
      901 aatgatttct tcaccaacgc atcgtctgag gcttgaaatt tactgaaatc ggcttcaatc
      961 cagccttttt caccttcaaa ccgcatgtac gggcgctccg tgtgatattc aatttctaaa
     1021 ccgttttcaa aacggtaaat cacgcgaaac tgcaacagga cattccaaaa gctgtctttt
     1081 ggcggataga ttcccgttcc ttcgatttca gttggccagc tcctgtcaag tccaagacac
     1141 catagcgccc cgttaagcaa gtgcgttccc caattggtga tcattccgtc acagtagttg
     1201 aggcgacgca tccagcccgg gcgacccttg agatcgtgtc gcgggtggac accgtttaag
     1261 gtgtacggga tgggtggggc ctgagtcaca ccagcacctt gccagcgagc gtaatcgagc
     1321 tcctcgggta caggcatttc aggttgggga ggacacgcaa catcggtttg cggcacgccg
     1381 actttgacgc gaagaatctt acccagcagg ttctcgcgca gaacagagta tgcccggcgt
     1441 gcgggggcac ccactcgaaa ttcgctgtca acacgaaata tccgattcaa gcgtgcggcg
     1501 gtctcggcga gtcgttttcc ctccgaaaac gttcgaataa tcggcttttc caacgccaca
     1561 tctttgccgg ctctcatggc gtcaacagcc atcacaccat gccaatgatc tggtgtggca
     1621 attaggacgg catcgatatc ctcccgtgca agcagatcat gataatccac gtaagtggca
     1681 gcacctgttg tgatctgata ttgctttctg gcgaattcgt cgatgcgggc cttggcctgt
     1741 tccaaccgcc agcgatcgac atcgcagagc gccacaatcc ggcaatcggg ttgattctga
     1801 aactggggaa tattcacatt aaaagtctgt cggccagttc cgataattcc cagacaaact
     1861 gcctcgctgg gtgccgtctg gccgaaaacg tggctcggca cgaaagtggg gatcagtggc
     1921 aacgctgctc cggaccattt taagaagact cgtcgcgaaa cttttggtcg cttggctgcc
     1981 ataggtttct tctcccaaaa tcgaacaaaa tcgaattcac caagcacggt ttttccaaca
     2041 tccactacga ctagggacag cggggcataa tctgcttcca ggccgtaagt gttcgctcaa
     2101 gtagctcctt tgccggaacg gttaagccgt agcactgaac gtagcatggg ccacggtatc
     2161 caagttcgca gtaaagggta tgaagaaagg cagaaaggtc gaattcacct tcgtccaacg
     2221 gctgaatcaa tttgtcccaa ttcatatttt gggtgtcgcc aatggcagaa ccgttgattg
     2281 tgaccatctt cagatatggg agtccttttt ccagggtcgg tcgaagctct cgcatctcat
     2341 gttgtttaag aaaatgacag aggttgaaac aaacgccaaa gtgggggtga ttgacggttt
     2401 tggcaatggc aagcgcatct tcgtccttgg ccacacgaaa acccacatga ggataaattg
     2461 ccaactggac gccagccttt gccgcttttt ctgcccaagg gcgcaaatgt tgagcgatta
     2521 ctgcgttatc gacgggcgtc cctgcctcgt gaatgttgag catgacaaca gcgttggaac
     2581 cctccaatgc tttgaagacg tcatctaacg gacactcgaa ctcacacgcg tcgggcttga
     2641 cgatcgtctt gatatagaca tttccaaccc gaagatcttt cctgcgaaac gtttcgaccc
     2701 attccggcag ttgctgggct aagcagatag cccccacaga ttgataaccc gtttcgcgaa
     2761 ggagcgctgc cagcaccgcc gggggcatct gtcgcatctc ttgcgtggtt gtcaagacgc
     2821 caaactccgg ttcggcggcc agaatgtgct gtgatgccga gctcaacaag cttgtcgcaa
     2881 caataagaga gctgagtggc ttggcgtaag cgatcatccg caagctcatc atgagtatcc
     2941 tcctgctgta ggggatagca aagtggaagc agagttgtta acaccggcac ggtgattgat
     3001 attcacccca acggtgcgcg ccaacctagg atagaggatt ttgaatgtca ccacaatgag
     3061 tttggcaacg aatcgtttgt ggtggatgcg ctagtcgtgc cgtatagcct ggggacaaag
     3121 ggacgttcaa tcgggaaagg cgggggccgc gctagccgcg caccgtcgga tccgagaatc
     3181 gcctgagcgg ccagggcaag gcggcggctc agcggtcttg ccctgccaaa gaccggaagt
     3241 cacccgcccg gttagaaaat ggcccaaggt cgcgatgagg nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn gttattcggc cgcatcggtt gcggtcgatt ggggattaag
     3421 ggtcggctcc ttttgtggag gtgttaccta tgaagctcgg ttacctgtgt ttggtgtcag
     3481 ttatctctgt tgccctggtg tcgggatttg cttgggccga accaggcaag aaagcgaaga
     3541 agggcgagaa ggctccgccc acgccggaag aattgttcgc tcaaatggat acgaacaatg
     3601 acaaggcggt taatttggac gagcttttgg ccatgaagca ggtgaaagga gataaagagt
     3661 ttgcccaaaa gcttctagcg ttatgggatg aaaacaagga tggcaaggcc accaaggacg
     3721 agtttaacaa gtttctggaa tcccaagcgc aacagcgcga aaagcagttt gccaaagtgg
     3781 atctcaatgc ggacaagtcg ctgacattgg acgagatcaa gtccatgaaa caagtgggcg
     3841 gcgacgaaac aaaggccaag gcgttcttgg agcgactcga tgacgacaag gatgggaagg
     3901 tttcattgaa ggagtttaca cgggtccgtg caatcttcca attgccgcca caggaaaaag
     3961 agaagaagga aaagaagggc gcaaactgat aggcggcggt aagtcacgct taccaagcta
     4021 atcttccctt ctgagaatcg tttcgattag tgagaaaagc cccgttgggc ggtgagccgg
     4081 cggggttttc gttttctcat gtatgccaaa ccggcctgtt ataacaggtt cctcgtcagg
     4141 cggggcttat cagtttgttc tgtccagacc ggccataacg cagggttatt gcgatgccat
     4201 tatggcgtca tcctcaaggg atgaaaggat tcgtcacgcg catcggtagg accgcggaag
     4261 acgttgtgat gcgaaccgtg ggtttgggct ccaacagtgg cggggctatc accctgtcca
     4321 ttgtgccttc tgtcttttcg ccgcagaaaa gattgagcac cactaccgga tgtggccgct
     4381 cgcccggtgg gcactcagtc ctacttccgg caccggcagg atgaagttgc ggcttaggaa
     4441 cgatcagacc gtttcgaagg cccgccaaat aagttgcata gctaagtcga gcccgccagt
     4501 cccattggtc accatatcgg gcaaaatcgg agcggtaaag catgcttgcc caaaactggg
     4561 cagaggatcg gtcgatttcg acgataggaa actgggcaaa atagggtacc atgtgtgttt
     4621 cgaagacgcg gtgacaataa gcccccggca gtcgcatttc caccttgcac acctctgagt
     4681 agggaaccac catcaaccta cgttgaacca cggtagaaaa tgtgccgggc gatggcccat
     4741 agagcatggt cgagagcaca aacaacggga gccagcagac gtgaatcatc tctgacgctt
     4801 ctcctcacaa gtgggggatg taacagtcgt tacattcagc ataatcagaa tcagccgatc
     4861 caacaaacaa tgaccgccgt acctgtttag gtacaacggt cgcggattct ttttaggcaa
     4921 gtgagccccg atgacttact cagaacggcc gctcgatata cgggctgcag tcccccaggt
     4981 cctggtcttg ctccaggaaa cacccctggt tacagtttcc gagccgctcc tgcgacattc
     5041 cggcggaatc ctggtaagaa gagccgctgg caccatcggc ttcgcaatcc gggacacgaa
     5101 tttcccatgg gtcgagaagg agataggact ggtccagaga aatgacggcc tcaaccacat
     5161 cacccagctc agtgatgacg gcatcccgga cggcgctcag tcctcccaca actccgataa
     5221 cgagaatcgt gatgagcaga atccattcga aggtgaggac accatcttcc tgggaccata
     5281 attgcacgag gactttctgc acggagattt cctcccttta ctagcctgcc tgtgatttgc
     5341 tcccgctcgc tcatttacga tataccacgc cttaggtttg agatcgctgt gcaaatggga
     5401 tgccgcacgg acaattcccg gcaggtaaat ttcacaatgc ggcataacta actctcggta
     5461 aggcacttac ggcgtaaatc ctcgcccggc cggaaggttg gcagaaaggc ccgcatccgc
     5521 gaacatgtag tccggcaaaa ggtttgtttc aaaatcacaa cgttttgtat cggacatagg
     5581 tgccgcggtt tacagcttga aacgaggtca gccacgacat gaccgtaatc accccgcggt
     5641 ttcaatagtt gtaacgatcg tagcggatgc aaaaaggaac aacgagcgcg tatgacgccc
     5701 cttctggaat gatcaggcgc cgccgtggaa ttcagaacct aagtttgaga gaacccacct
     5761 aataactgcc ccccaagcga ggcgaatctg tttttcattc ccacccaggc gggggttact
     5821 ctacgggcgt ttgcaccgtg ctttgtgtgg tcaagtagtg ccagcaaaaa ggaagacgag
     5881 ttatgaataa gctcaccaac ctgcttctgg aaaaggaaat cctgctggag agcggtacga
     5941 acgaactcga aatcctcgta ttcgacgttg gtcaatacgt ctttggcgtt aatgtggcaa
     6001 aagttcgcga ggtactgccc gcacctacca tcacccattt gcccaacgcc caccccagca
     6061 ttcggggagt cttcaagctg agaaatcaag tcgttccgtg tgtctccttg gcggattact
     6121 tgggtcaata tgacggtggt gacgatcggg aatcggtatg cattcttaca gacctgaacc
     6181 agcagcaaac ggcgtttctt gtagatcgtg tggagaggat tcaccggctg agctgggaaa
     6241 acgtgctggc tgtaccgccg cttgaggatc tcgctcagac gcctgtcacg gcactagccc
     6301 gctgccaaga ccgcctcatc cttatgctcg actttgaaat gatccttgac caggttaccg
     6361 aacactactt ccggacagaa tcagtggaaa atccggcctc gctgccccgc gagcaagccg
     6421 tcattttgtt ggtggaggac tcgccgaccg tgcgcgaggc catcacgcaa acactttctg
     6481 ccagtggata ttcccggctg gttgtatttg agaacggaaa gcacgcctgg gagtggctgg
     6541 aaaagaaggt caatgaccag tcatcgcacg gttgcctttg cgatctcgtc attgccgata
     6601 tcgaaatgcc ccaggtagat ggtttgcatc tgacaaaaag gatcaagagc catccgcagc
     6661 taaaggacat accggtcctg ctgtactcct ccattatcac gccggaaaac aggcggaagg
     6721 gggaggccgt gggtgccgac gctcaggtcg ccaaaccgga cttgtgtcat gtggtggaat
     6781 acgccgacaa ccttatcttt ggccggcggg acaatagcgg gtcacatttt agcaacgtaa
     6841 aagacgtcag caatgtggaa cgcactcctg ctgtggcgaa tcctacttca atgccacaag
     6901 ccgagacgca ctcgaacgat gagcagtgcg tgtcagccgg actggccgag gagccaacaa
     6961 gcatggccga accccaagtt gctgggtcca ttgaaccgga agcaaatgtg gaaaggccat
     7021 ccggcgccgg cgacaaaaac tgtctgagcg gtcactatga ccaatgtgtc gttagcccag
     7081 cggttacgga cgagcacgaa tcgatggaca attgttcgag gcaagtcgag gttgttgcga
     7141 gcgagtcctc cgaatcggag gtttccgacg cggacgacga cgaggccatc attggtttgc
     7201 ggccagacgt ggtcgagaac gagcgactgt ggaatacatt tgcttgtgag cttcaacatc
     7261 gctggcaagc actatcggag ctgcgaaggc agatttctgc gaagggagag acgttcgagc
     7321 ccgccaagga cttcgacatt gttgttgatg tggcgcggat actccacacc attaagtccg
     7381 cggcgatggt tgttccaatc gagattgtta ctcggacgac tcatgccctt gaaagcctga
     7441 ttgaagccgg ttccgatcgc aacgagtggc ctgcccaggc gtttaatcgt tattgcgatt
     7501 ggctggaaat gttcctcaat ccggttgatc agacgtggga ttcttggaga gagactggaa
     7561 actacctggc agccgatctt gaacaacagt tggcccaacc tgcggaagca ccccagacca
     7621 cgacatcttg ccaaggataa actgtggcta tcgcacgtgg cacgagacaa ggcggaccgt
     7681 gcaccggacg ccgccgttgg ttctgttcat ctggctgagg tcgatttacg acacacgcca
     7741 aaaggagcgg tcaatccgca ggagtccctt tcagtaccgc cgaacacggc aacaccttgt
     7801 taacgaaatg tgaagtgttc gatttccagg cgaaccaggt gttctgccac ggctgctgcg
     7861 gaagcaggac gccgttccgg tatgggtgac atcatctggc gaattaggcg actcacttcg
     7921 tgtgagacag aaggattcag gatgcggaca tccagaactc gccgttgccg atgaacccgg
     7981 gcggcttctc gaaggctgcg aacgcaatat gggcgttggc ctgtcaggac ttcaaagaaa
     8041 atcacaccca ggctataaag gtcgcttttg ggtccccagg gggcatgtcg aaagatgcat
     8101 tccggggcca tgtagtccaa cgtgccgacg acttcccgcg gagacaaact cccttcttcc
     8161 caccgttggg caaaaccaaa atcgatgatg gtgacatgac cctcgggatt gaccatgatg
     8221 ttggacggct taagatcagc gtgaagccaa cctgccccgt gcagtgccgc caggccttcg
     8281 gctatctgtc gaacaaacca aatggcgtct gcagtaggaa tcgccccgcg ttgactaagc
     8341 aattgttgta aggacgtccc ttctagccag ggcataacga catacggcca atcgccagtc
     8401 aatctccaag cgaggatggg cacaacgtgg ggatggacca ccgtccgccc gacttccgcc
     8461 tcacgggaaa ggtgacctcg ggcgatggca gtgtcagtca gatctgtcct caatgtcttg
     8521 atggcatacc gagggggccg attgggtgga ttcgacgcca cagttgcccg aaacacggtt
     8581 gtccatgtac cctgcgcgac gggcatgagg agaagccact cccccagggt acgcgggaca
     8641 aaaccttggt ctgccctccc tatgttttcg aagtgcgatc gggcttggat ggcgtaagtt
     8701 aacatagaca cattgcgttg cgagtcaaac cccggctgac aaactctctt gctcgttcat
     8761 cggccaacag cggtaagagg ctccacgtgg gcaaagccgc gtgagatggt gggaacaatc
     8821 cttccaacag tcataatgcg tgcacaccga gcaaccgcca gtaaccgacc aaggccagaa
     8881 actcgataaa ggccaaaagt ccttacgaaa atcccgcgca atccgtttgg tcgcaggcta
     8941 taatggacat ggaaaaacgc ctttttcagg cggtgaactt tgacagcaaa catttgccat
     9001 agcgctttga aagatatggc cttgcgcgaa tcgacaaaat gcgacgatca gtccaaaagt
     9061 atgcatactg acgacactac agccccgttt gtccagatgc gcaatgtgag caaacttttt
     9121 gtcatgggcg acgtggttgt cgaagctctt cgcgatatca ctctggatgt ttatcatagg
     9181 gaattccttg tcgttttggg gccaagcggc tcaggcaaat ccaccctgtt aaatcttttg
     9241 ggcggactcg acacgcctac agaagggcag gtcctgtttg aaggacgcga cctggcagaa
     9301 tttgatcggc gagaactgac gtggtatcgg cgggaaatcg ttggttttgt gttccaattc
     9361 tacaacctgg ttgccaatct tacggcactg gaaaacatca tgctcgctgc cgaccttgtc
     9421 agtgatcctc tggatcccgt ggatcttcta gagcaggtag ggctagccga ccgggcggat
     9481 agctttcccg cccaactttc cggtggcgag caacagcggg ttgccattgc ccgagcgatg
     9541 gtcaaaaatc cgccgctaat cctttgtgat gaaccaacgg gcgcgttaga tgatatgact
     9601 ggacgtcaaa ttctgaggtt gctactcaac ttgaaagagg atatggggaa aacggtggta
     9661 gtgatcacgc ataacacggc gattgcgcag atggcggatc gtgtgatccg actacgcagt
     9721 gggcgaattg tagaggtcac ggaacaagcg tcgccactgc cgcccgaaaa gattcagtgg
     9781 taacggtgac catggttctc aacaggacgt gacgacgcgg agacaaagag atgaatcccc
     9841 tgcatcgaaa actcatccgc gagttacgag cttcaggggg tgtcatattc tctgtcatct
     9901 tgatcacggc gattgggacg gcctgtttcg tggcgatgag atcggcccac cgaagtttga
     9961 gccaggcgcg tgatcactac tatgccaaag ggcggatggc cgacttttgg gtctcggtaa
    10021 agagaatgcc gaaatcggag ttgagacgtc tttccaacat tccaggggtt gcggaagttc
    10081 atccgcggat tcgttcaccc gtcactgtcg atttgccgac ctcagagaag cctctcgcag
    10141 gggtggtgtt gagtctcccc gacaggggtt tgccggcgct caatcaaatc cagatcctgc
    10201 ggggagaata cttcacttcc cgacggcgaa atgaggtcat tgtgaacgat gtgttcgcca
    10261 atgcccatcg gctccgcctc ggtcaatcta tctttgtcaa cattgcagga cggcgacaag
    10321 agctggtgct cgtcggtacg gcgtttagct gcgagtttat ttacccactc ggtcgagcgg
    10381 ccattgttcc ggaccgcaca ggcttcggtg tcttctttct caaggagagt ttcgccgagg
    10441 aaatcactga catgaagggg gcaacaaacg aggtgttagg acgggtattc cctcggtatg
    10501 ccaactccaa gagggctctc cttcacgaaa tcgaacggcg actcgacgaa tttggggtcc
    10561 tcgccacgac acctctagag gattacccct ccaatgcatt tttaagccag gaaatcgaag
    10621 gcttagcaac gtttgccttg ctcatcccgt tgatctttct agggatttcg gccgtgattc
    10681 tgaatgttgt catgactcgg cgtgtcgagc aacagcgcat cgccatcgga acactcaaag
    10741 ctctaggtta tggggacgca gtcatcggct ggcacattat ggagtttgct ttgctcgcgg
    10801 gcgtgatagg ggcagccatt gggtgtggcg tcggccagtg gttgtcggcc gggatgacaa
    10861 gtatctaccg ccagttctac gagtttcctc ggttggatgc gcctatttat tgggatatcc
    10921 atttagaggc gatcctcgtc gggctcattt gcgccgctat cgggggttgg cggggagcga
    10981 gaacggccct tgctttgcct ccggcagcgg ctatgcgacc acgggtacca gcttcagggc
    11041 ggcatatcct cctggagcgg ttcgggtggc tgtggcggca tgttcatccc gcctggcgga
    11101 tggcgctccg gtacatgttc cgccacccca tccgaacggc aacgtcacta cttgcttgtt
    11161 tgctgggtgc aagcgttatg gcacttattt tttgcatgat tgtgggggtc aaccacttga
    11221 tggactttca tttccggtgg ttgtatcgaa gtgatgtgac gctgtttctc aagagcgaac
    11281 gcggtagtga cgctttgatg gaagtggccc atttgccggg cgtgagaaag gtggagccac
    11341 tcttgggcat gcccgtgaca ttaaggcatg aaggcaagga gaagttgatt tccgtgaccg
    11401 gcattcagcg gacggccaca ctgaccttgc cccgagatcg ccaggggcat catattacta
    11461 tcccgcccac ggggttcctc atgagccgtc aactggccaa gatgatgggg gttaaacccg
    11521 gcgaatacat cgattggcaa ccgatgcgcg gtgatcggag gtggtatcga acacaggtgg
    11581 tcaatctgat ggatgactac gtcggtaccg ccgtctatgc cgatttgact tttttgagaa
    11641 aatgccttgg cgaagggtcc gcccttaaca gcctccagtt ggcaattgat gccggtcacg
    11701 aacaacgaag ggcattcgtc aagaaaatcc gggattggcc cgaaatcgag gccatgatct
    11761 accgcgccga tatggagaac aacctacgaa agaccctcct tcagaacatt tgggtcatcc
    11821 tttcaattga aatctccttt gccggggccc ttttttttgg agccattttg aacggtgcca
    11881 tcatttcttt ggaggagcgg cgtcgcgagc ttgcaacgtt ttatgtgatg ggctacacgc
    11941 cttggcagat tggcggtttg cttgggcggg aatccgttgt tgtggtcatc ggcgggctga
    12001 tcttggggat accggttggc tatttctttg ccaggctggt tgtcgctagt taccaaagtg
    12061 aggtattgag gatgcctctc ttgtttccgc ctcaagtatg gatccaaaca gccatctggg
    12121 ggtgtctgtt tagcctggtt tcccatttcg tcgtccagat catgatccac cgcagccgat
    12181 gggctgaggc ggtcaaggtc gccgaatagg catgattttc gcggaaaggc gtgttcggag
    12241 tactagcgtc atgctcgaaa aactagttaa ggggttctga agaatgtcca ccataaaccg
    12301 cgggctgcct tccaaaagca gaaacaagaa gttcaagaat tgggttggac ttggtgttgc
    12361 aagtgtgctc gtgcttgggg gcatagcatt tattttgtcc tctcggactt cctcccgaga
    12421 gatggagaca acaccttgtc ggcggggaaa cattcaagag tttgtcagcg agaaaggagt
    12481 gacacgactg cgggacaccg tgctgctgac gatgcccttt tccggtcgca tcgaggccat
    12541 tccatggatc gagggaacgg aggtgaaaaa agggcaagtg ttggcaagga tcgtccccag
    12601 cgatgttgag caaatgttgg aagaagccag ggccgtggtg gcgcggattg aggctgccat
    12661 tcgaacgcaa aaggacaatc gggtggagga gacggccatt gctcaggccc aggcttatgt
    12721 ggaaagcatg aagtcaactg tcgcggctgc cgcagcccgg gttgaagcca gcgaagctcg
    12781 ttacaactac gcccaaaagt acgcggcacg tattgctggt ctcgttcagt ctggcggtgc
    12841 ttcgcaggac gaccttgatc gcacggaact actgaagatc gaaagtgccc tttcttacca
    12901 acaggaccgc ttggttcacg ccgccctggt ttctctccag gccgccacaa atctgttgcc
    12961 tgtacttatc cagaagtatg tggagcggaa agccctcgtt gaagatgaat tggtccaaca
    13021 actggcagag gccaaggcca ggctggcgat ggttgaagag aacgctcgtc gtgccaccat
    13081 ggtcagcccc tacgatggta ttgtcctaca tcgctacgtg agccaggagc gatttctcgc
    13141 tgcgggaacg caactgttgg agttgggacg ctggcacgac cttgagatcg aaacagaaat
    13201 cctcacccaa gatgccgtgc gcatcaaaca aggcatgttg gtggatgtat tccttggaga
    13261 agaccttgcc aggcccgtgt gtcgcggcaa agtgagtcgc atccatccgg cagccttcac
    13321 aaagctgagt tccttgggag tggaagagca acgggtcaag gtgaccgtcg aactgcgccc
    13381 ggaagatata gacttacttc gacgtgatta caatgtaggg gtcggatatc gcgtctgggt
    13441 ccgggtcatc ctccaggaaa agccaaacgt gcttctcgta ccgagaattt ccgtggtccg
    13501 cgaggaaact gatcgttggg tcgtttgggt atggaacaag cggcgcgtgg agcgtcgggt
    13561 cgtcaccgtt ggcatcatga acgactcctt cgcggagatt ctttctggct tggccgaggg
    13621 cgaagaagtc gtgttaacgc ccgaaacggg aggtgcagca tcgcattaat gattaataca
    13681 aagtacaatg aatagtagtg tgccagcggt gggttgcatc ccaattgcgg gggagttacg
    13741 cggccagtcg tggacttggt ggcttgccgc gatcggcgtc aggcgtcatc catgggaata
    13801 cgcttttatt gtccaaacgg ccataagctc catgtgaagg cctttcaagc cgggatgcgg
    13861 ggaatttgcc catattgtgg ggcaaaagtg caaatcccgt tgcacagtac gcgacccagc
    13921 acaaaggaat taaaggcccg gcgagccgca ggtggtccgg agcatgctgc gattccgagg
    13981 ggcgacacag caatgcccga aagccggcaa cccggtgtgg actcatcgcc ggaaaaagcc
    14041 ccggacgggc aaggggtgct gcccggtgag caagagcaac tcacgccgtt tatcgactta
    14101 gacaaggtgt tggcagcacg taatcttggt gaccaagggg ggctgcgatc ctcaccaagc
    14161 ccattagagg gcgatttggc gatttccacg gcagagagtg tacggtctcg caattccgtg
    14221 accgaagaat ggggttcgcc aggtgaaagt gccaggagtt tcgaaaaaga tcctttggca
    14281 gagccgaatg ttgtctggta tgttcggcct cccagcgggg gccaatacgg cccggcagca
    14341 cccgaagtcg ttcgcacgtg gattgcagaa aaccggatca cacctgatac ctatgtctgg
    14401 cgtgaaggct ggcgagattg gcaattggcg agagatgtct ttcctgaggt gcttgctaag
    14461 gcccttccgg aagtccccgt tgaagaccag ccggagcgcc gtctcatacg caggtctgtg
    14521 cgtagttcat gggcacaata cgctattgtg gcgtttgctg cgattgtcat ttgcgttctg
    14581 ggcggtctgg tttggttatt cgtctcgcgc agctctccat agcggtcagt tgggggcagc
    14641 ccccgcctat tgacggctag attccaagaa gaataatggg aacctgagcg acggtttggc
    14701 agggcccggt ggcatgtctc tgtaaccgtt tttcaacctc cgacgtgatc tgagacacct
    14761 cttgtatgaa ggagttttct caatgacaga tgcacccaaa caaaaccggc gtggttttct
    14821 cgtcagttcg ggaattacag ccgcaacgtt gatgatggct cccagtgttg ttcctacgac
    14881 ggcacaagct aaagaggcct caaccccgag ggcgccgcgc tttcggttgg cccttgcttc
    14941 ctatacatta cgtctcttcc ccttggatca ggccttggcc atgaccaagc gggttggctt
    15001 gaagcatatc tgccttaagt cgttccactt gcccttggat gcctcggctg atcagattga
    15061 ggcggctaag aagcaggtgg ctgaggcggg tatcgacctc tacgctggtg gcgtcatcaa
    15121 catggcaacg gaggccgagg ttgatcaggc atttcaatat gcccagctag cggggatgcg
    15181 ggtcatcgtg gcttcgccac gtccccccgt tcttgaacgt gttaatgcca aggtcaaaga
    15241 gacagggatc gcagtggcaa ttcataacca tggcccaggg gataagttct tccccacccc
    15301 tgaaagcgta attgagcaag tgaagaacct ggacaagcga attgggctgt gcatggatgt
    15361 cggtcatacg gcccggattg gagcagatcc catccaagat gcccgccgtt ttgccgacag
    15421 gcttttggac gtgcacatta aggatatcaa cgtagctgcg cccgaaggcc gttgcgttga
    15481 atgcggacga ggtgtcctcg atctgccaaa attcctcaaa gtgctcattg aaatcgacta
    15541 tgcaggaatg gtggcttttg aatacgagaa ggatgaaaaa gatccgttgc cgggattggc
    15601 cgaatccgta ggttacgtgc gaggtgtgct cgattccctg ttgggcattt cggatcggtc
    15661 ggcccaattg gaataatgcc gcgaggtttt atccccgaaa aagtttgccc tttgctcggg
    15721 tgtcttgggg tcaaacgtaa gttactcgtg tctgaatgat cgtgagctga tttctgacca
    15781 ggcaaacgga agtttggccc aactcacagg aaggggggcc tcgatcgcca ttgattcctt
    15841 tcggacggga tgtgtgaact cgatccggcg cgaatggagg gcgatccctt ccgcaaaggc
    15901 ctgttttgcg ccgtatttct ggtccccaag gatggggcag cccataacgg ccagttggat
    15961 gcgaatctgg tgcttccgtc ctgtgtgggg atgcacttcg agcagtgtca acccgcggga
    16021 cgctgcaagt tgtttatacg aaagcacggc ttcctggccg tcagggtcgt gggcatgcgt
    16081 gacgtggact ctgcgaaaac gttcatcgga tcgcagaaag tggcgcagtg tgcccgcagg
    16141 ctgaggaacg cgcccttcga cgatggccca atatattttc gagacactgc gggttcgaaa
    16201 ctgctcggtc aagcgcgccg cggccttgga cgttttggca atcaagacaa ctccgctgac
    16261 gggaacatcg aggcggctta caacccctaa ataaacattt ccgggctttg catctcgtct
    16321 tttgagccag ctctttaacc aactaagaag tgtctcctga tcctcgggta accccatggt
    16381 tgccaaaccc gcaggcttgt tcacagccaa aagatgattg tcttcataga ggatttcagg
    16441 atcgcccata accaatcatt cggcgatgac tagttgcaac agttccacac tgccttctgg
    16501 ggtgctagcg ccagccgccc agggcaaggg aaaaagggga caccattcac gaacgtttgt
    16561 tgtacgaatc tcgaagattc ttcacgcgag ccgaggtgaa caaccacgac gtgaccgcgg
    16621 tccgctaatc gtacgttcac aagacccctt ttgtgctggg gattccttcc ccgcggggat
    16681 cgcgttcaat agccatgcga atagcccggg ctgttgcttt aaagatcgcc tcggcgatat
    16741 ggtggttatt tgttccatag tggacgcaga tgtggagatt ggcctcggca cagtgagcaa
    16801 aggcttgcca gaattcccga atcagttccg tctcgaaatt gccaatggtc tccctggaaa
    16861 actgggcgtt gaagacaaaa tacgctcgcc ctccaaagtc cacggcagcg gttgccaata
    16921 cctcgtccat gggcagtgtc caatggccgt aacggcggat ctccttcttg tcgccaaggg
    16981 cgttgcgaaa acaggtgccc aacacaatgc cggtatcctc caccgtgtgg tgttgatcga
    17041 cgtgcaaatc gccgcggcat ctgatttcca aatcgatgca cgagtgcttg gcaaatagcg
    17101 tcagcatgtg atcgaagaag ccgatcccgg tgtcgatccg tgactcccct ttgccgtcaa
    17161 ggttgatgga agcggcaatc tcggtttcca gcgttttgcg ttcgatttgg cttgtgcgcg
    17221 gtgtcatatt gctatcatcc aatcaaagaa tttcgcggag gctgttgagc agaccatcga
    17281 cctcggcgtc cgtgcccacg gttatgcgca gcccatcccc ccatcctggg tagttcatat
    17341 accgaacgag gactttccgg gctttgagcc tctcgtaaat ctccttcgag gcacgctcgg
    17401 gatgcgaaca ccaaacaaag ttagcctgcg aggggagcac gtcaaatccc agaccccgca
    17461 actctcgcat caaacgttct cgagtcgcca agatcttccg ccgcgtttcc tgaagccatt
    17521 cttgatcact cagggccgcg gtggctccgg caatagcaag cgcgtcgcag ttgtaagaat
    17581 ctttgacctt acgtagttct cggataatgt ggggctgggc aatcaagaaa ccaaacctca
    17641 agcccgccaa agcgtaagat ttgctcaagg tccgggaaat catgacgtgc tcgtattccc
    17701 gaaccaagtc aaggcaagac tcttcggcaa aatcgacata agcctcatca atgaggatag
    17761 gacaccgcaa gtgctcaatc tcgcgtttga tcacatcctt gctaatcatc gtccccgacg
    17821 ggctgttggg gttggcgagg taaatcagct tcaatcgttc cttttctttc cagaatgagc
    17881 ttcccagact ccaatccttt tcaaacaaaa cgaactcact gtccgcgttt tgcagttgcg
    17941 caaggaccga gtaaagaagg tagcttggat aaggataccg aatctggtca ccttctccca
    18001 caaaagcgcg agtgacgatt gtcaaaacat cgtcgctgcc gttgcccgca agaatccagt
    18061 caggatccac tcccaggacg gcagccgccg ttttccgaaa tttggtggcc aatggatcgg
    18121 gatacttctg aaggccgcgg cgaaggacct ccgtgatggt ctcggccacg cgaggcgacg
    18181 gcggataggg gttttcattg gtattgagtt tgatatagcc gtcctcctgg ggttgttccc
    18241 ctgggacgta accttccatc ttcagaatat ttggacgcat gaacgaataa accatacaac
    18301 cgacggggta attcatgaaa taagccgatt tttcttgcca aacctctctt aaccgcgacg
    18361 cacggcgacg ctttcccggt gggccgttaa cccttccttg gtcgctagat aggccacatc
    18421 atctgctaaa tgttccagtc cggactggtc aaaataaagg atacttgttg ccctgaggaa
    18481 atcattagcc gacaaggcac tggcaaatct cgcggtcccg cctgtcggta gcacatgtga
    18541 cggccctgcc gcgtaatccc ccaaggccac gggggtgtag tgtcctaaaa agattgcacc
    18601 cgcgtggggt attctctggg ccaacgcttc agcattgcgg gtggagattt gtaggtgctc
    18661 aaccgctaac tcgtcggcca cctggcacgc ttgatcctga tccttgacca acactactgc
    18721 gccgaaatcc tccaggcacc gtctcgccag ggggccccga tccacctttt ccaaacactc
    18781 ttcgactgtc atgacgatcc tgcgtagcac gtcctcatcc caaccaatca agacactcac
    18841 gcccggtgag tgttctgatt gggcgatgag atcgaatgcg ataaatgtgg ggttggctgt
    18901 gtcatcgacg ataatggcga cttcacttgg tccggcgatg gagtcgatat cgacctcacc
    18961 aaaaacatac ttctttgcca gggccacaaa taaattgcct ggcccgacaa ttttgtcaac
    19021 tcgcggtgtt acctccgtcc cataggccag ggctgcaaca ccttgtgccc cgcccagcga
    19081 atagacctcc caaacaccaa gttcggcgca tgttgccaaa atgtcgggat tgaacgcccc
    19141 aaatcgggtc ggtggggcta ttacggcaat ttcccgcacg ccggccacca gtgccggtat
    19201 gactgtcatc aaaactgtcg atgggtaggc cgccgcccct ccaggcacac aaacccccac
    19261 ccttgtcagg ggaagatacc gctccagcag gtagccacct ggtcgagtga cccggacgtc
    19321 gtggtgcaaa attgcccgct gaaattcctc gatattgctg cgaattcgtc ggatgcactg
    19381 gagaaacgcg ggatctgcct gttcgtgtgc cgccttgatc ctgtccaccg ggaccctgac
    19441 cgtctccggg gtcaactcca ccgcgtcaat cttcctggtg tattcgatcg ccgcctcaat
    19501 tccgcgggtc cggatatcgt ggcatatcct ttccacgact tgagctggcg tcaaaggctc
    19561 cccgaaaatc ttgaccgtct tctcctgcga ttctggcgtg acaatgtctc cacggagact
    19621 cagcctcgcg cggagttcgt caaacacctt tcgccactgc tcggtgcgac aatcgaccca
    19681 acggatatta agcattctcg gcatcccacc ataaaaagga cctggcaacg cgactgagag
    19741 taaccaatat ttatcaaggt agcttgggaa agccgttact ccaagtggcg gaaagtatgc
    19801 gcctggcatc ggcctggtca ttgggtgaca tctgttgttg acagatttcc caaacattgg
    19861 gttcggaatt ggccgttgga cgagtggcct ggcagcagcg gtgtgcggta actagtccgc
    19921 tttatggtgg tacaataagt cgcaatagtg cgtaagtgac tcataagaag cggcaagaaa
    19981 cggtggcttt ttggtaacga tcaattcagg gtaacctcgg gtaccatgcc ggcgggttga
    20041 aggcgtaccg gcgtagggtg accgagtggg cccgcacttg cgcagtatcc ttagcgacga
    20101 tttgtcagag aagacgcatt cgtgagtgca gaatttgtca ttcagtgccc cacctgcttg
    20161 gctcgcttga gggttggctc ggcgaaggca gcgggcgcca ttttgccgtg tcccaagtgt
    20221 gggagcatgg ttgctatccc cctcctagct gggattccaa acgggtctgg ggaggagttg
    20281 tcagggttgg gcaagctgac tgaggttcag gagaaggagg aggcaaaaca aaccgcgggg
    20341 gatagcgggt tggttgaagc ggagcaggca gcggaccgtt ccaacgaaaa agcggtgggg
    20401 atttccgatc aaaagaggca agaatccatc gccgaacagg gaaaggatga ctcagcggaa
    20461 aaacctcaga ccacgggcaa ggcagaatgc gagaacatcg cggaatgttc tgccgaatcg
    20521 tacgcgtaca agatccgccg cattgtcttt gccgtttcgg ccctgtgtgt tctggtcctg
    20581 gcggtgtggt tttggtggat tgggcatggg ggcaatgatg ttcccgaacc cccggatatc
    20641 caaaagacgg aggagatcgc aaaaggcgac ttgaccacgc ccacggctgt ggaacaacaa
    20701 gaggagggcc acgcctcttg ggtccccagt ggtgcggact tctttatcta cgaagatacc
    20761 agcgctgatt taaccgggca tgatcggggc gttgtcgcta atgtctttgc gttatctgag
    20821 gcatctgcga gggcggatca gcttttatcg gttttagggc tcaaacgcga agtggtggcg
    20881 gggcggttta tctcggcaga cgcgcggttt gcgactaccg tcgtggtttt tcagttgaaa
    20941 caggatcagc gggcggaaca gctcaatgtc ttgggggagc cgtgggaaaa cgttgggccg
    21001 gtccagttgc ggcaattggc cgttggtcct gaacgttttg cctttgctgt tgtggcagag
    21061 aactgcgtgg caattggcca gccggagatt ctgaagcgat tattgtcagg cgaccatgac
    21121 cccaacatca aagccatttt ggagcaaaag ctgaaatcca agccccggta tctcatggtg
    21181 attcggccca cgctcattcg ccatcgtgga atgatggcgt ggtttgacgc ttggccggag
    21241 atcgaccaat tgtggcagaa cctttgggcc ggtgcgaacc tatttatctt ttcgatcgaa
    21301 cgttcggaac aacaagtcat ttggacatgc gaatggattg caaaaagtcc cgatgacaaa
    21361 gattccctgg aaaagagtgt gaggggactc ttgacgagag tgnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nngtaatggg ggaatggcgg cactgggcgg agatcgcgat
    21541 gactcggcgt catggccacc tgggcaaggc gatgtccgag tacgaaagta agcaacagat
    21601 gttccccatg gcagccggcg gcggtgttct gctacctcca gagacgcggc taagttggat
    21661 tgccgattta ctgccctatt tggggcgcga ggattgggcg aaggccctgc aaaagggtta
    21721 ctcgtggaat agtgcccaaa accaggaagt gacccagcgc tacttgccgg atgtcactaa
    21781 tccgctctta gggcccacgc agtcgacggc aggctatttt gactctcatg ttgtgggtgt
    21841 tacaggtgtg ggggaggata gcggcgagtt accgccgagt catccgcggg caggggtgtt
    21901 caactatcgc cacccggttc gccggtcgga tattaccgac ggtctatcca acacaatcgc
    21961 catgctggga gtagaaaaca agcgtggccc ttgggcggcg ggcggactat cgacggctag
    22021 ggggttaacc aaacctccgt atgtgaacgg ttcggatggt ttcggctcgg ccatgccgga
    22081 tggtatgctt gccaccatgg cggacggttc tgttcgcttc gtgcgaaagg atgtcgatcc
    22141 ccgcgtgttg gaacaattgg caacgatcgc cgggggagaa ccagttaccg ttgatctctt
    22201 gggttctcgg gcgagcacgg caaaaggcga agctgcgcca tcggggaaaa acggtacgtc
    22261 cggcccagta ggcgtcgctg cggcaaggct tccctatatc cccaccatgg gaacctggcg
    22321 cgacccgtgt ggttcctcct tggaaacggc gtccgacaat gatggtttct ccgacacgtc
    22381 ttggcggcga cgtgtggcac aggtgcgttt ccgcgcttcc ttggccttgg ccatcagctt
    22441 gttaacgcaa tggtcgcgtg tccctgtgtg tgtggatcct gaatcggctg agtggttacg
    22501 cctgaccgga aatgaagaga ttcaagttga gatgcagaac gtcacgttcg aagatataac
    22561 aagagccatg ggagaagagg taggcttgtc acttgtgcca tgtaaacagt cggtggttct
    22621 tgttgctgcc gatcgaataa gggatcacgt ccgttacgaa tttcagatgg aggaaggtga
    22681 atcagggact ttcccatcgt caaccacgtt agagcaagtc tgtgattggt tcaatgaggt
    22741 gagccctggt ggtttcgaca aaccggcaca ggttgcaaaa gagggtgatc gcctggtaat
    22801 tgatgggcct tttcccgttg ttttcgaaat gcgaagaatg ctagagcatc gggctgctat
    22861 gggcagcacc aatgagcgtc tggatgccga agacgaggcg acgattagtc ttaacttctt
    22921 cgagccaaca aggctgactg atatcgcagt agccatcaat cggaccgcag caaacaaggt
    22981 gttcttcgat tggacctcgc tggccagaat tgacgtaaca cccgatacgg aggtttctat
    23041 ttcggcgacg gatgagccgc tctcgggtgt cctcgagcga cttgccgagt tgatccaagc
    23101 tcaggtccat gcgtgggacg atgggccatt tgttgtttgc gcacccaatc ggctgacttc
    23161 gggcgtttta cggatttatt atgtcggtga tatccttgag aaagggacaa ctccgggaca
    23221 gttgcgcgaa caacttataa gggagtgcgc tccagaaagc tgggccgatg gcggggggac
    23281 ggggatgctc atctatgatg cacctgccca ggcctttgtt gtgcttaaca gtgccatagt
    23341 acatcgtgag atatcctcct ggttacaaaa acggcgagag ggccaacaag ggcaatgagc
    23401 ggagagtact tacacgtggt atctcctgcc tgcttgctga agcccaagca taaatggggt
    23461 cggagagtat ggacaggcga cgcaagtttt tgagacgatt ttcaaaacgc tctctattgg
    23521 cggtggtaaa gcgatatcgc gacgctgtgc ggaacgagcc tgccgtccat cgtactgccg
    23581 cgtggtggtt ggcgtattat cagcgaagtg tggctgaact tctcctcatc atttgggcgg
    23641 ggatgggctt attcgtcggt tttcggcagt tgggtgatgg tctctcgttg tggtttaaca
    23701 cgtcgtctcc tcgttcatac gccttcatag tggaggtaaa ctctgccgat tggcccgagt
    23761 tgacctgcct gccaggcatc ggggaaattt tggctaaacg gatcgtgatg acgcgccagg
    23821 aaatgggcag tttttcccgg agtgaggatc tgaggactgt taagggtatc ggccctaaga
    23881 ctgttcgcaa gatagcgccg tttctaagat tcaacaatga tcggcctatt gagcgattac
    23941 ctgccaccag gaagtgaaat ccaccctgag acgtgcggag taaaaaaggt gtcgggtgta
    24001 ccagcagggc agggtgttag atttttcgtt cttgaatgtt tgtagtcatc gcctgtgatt
    24061 ggaatgagat catggcaaaa gtgttgataa cctcaggacc cacccgcgag taccttgatc
    24121 cggtgcggta tctctcaaat gcctcaagtg ggcggatggg cagggccctt gccgaggtgt
    24181 ttctcgagca tggccatcag gtgttcatcg tttcgggtcc tgttgagatt gaatacccca
    24241 atggcgcgga tgtggtgccg gtcataacga cagaagaaat gttgcaagaa tgtcaaagac
    24301 gtttcgaaca atgtgacggc ctaatcgggg cagccgcgcc ttgcgattac cgtcctatga
    24361 ctttttcccc cgagaagctg cgcaaatcgg gtcacaccct tgcggtggaa cttgtcgaga
    24421 cgcctgacat tcttgcgacg cttgccacga ggaaagggtc tcgctggatt gtggggtttg
    24481 ccttggagac ccatgatgtc cacaaccgcg cgatggaaaa acttcgaaaa aagcaatgtg
    24541 atttgatcgt cattaaccga ccagccgcaa tcggggcaca gaccactgaa gttgagattg
    24601 cagatgccaa cggcgttatt ttgggaaatt ggacaggttc caaggaggcg attgcccgac
    24661 ggctgtacga aattgttgcc caacgttttc tcagaagggg ataagaagcg tttggcggcg
    24721 tcttgaccac cgaaaagcgg ttcggtgaaa taatgtcttc tgttaatcgt gagacttggg
    24781 accaaggtga ggagatgctc catggccgat cgccatgatg ttgtatccgc gggtattttg
    24841 tttgccgatg tggcttgcga accgatcgag agattgcctc gtgctggaga gttaatcccc
    24901 acaaagcgga ttcaactggg cttgggaggg tgtgccgcga acgtcgccgt ggacctgaaa
    24961 aagctcggca tctcagtggg tgtaagcggc tgcgttggtg atgatttctt tgggcaattc
    25021 atcagccagg tgcttgaccg cgagggtgtg gatacaggtg ggattcgttc tgtgcccgga
    25081 atgaccacgg cggcaacgat gatggttaac gtgaaaggtg aagatcgccg attcatcagt
    25141 acgccagggg ccaatgtggt ctttaaggcc gaagatattc ccacggcctg gactcatgct
    25201 gcccgtgttt tttacgtcgg tggatatcta atgatgccgg ggctggagac cgatgatttc
    25261 gtgtaccttc tgcgcgacct gcgggcaaaa ggcgtgatta cggtggtcga tgttgttttg
    25321 atggagcgac gggatttttg gccaatcttg agaaaagtcc tccccgaggt cgattatttt
    25381 ttgcctaacg acgacgaggg ggaaattatt acgggtgtag ctgatccgtt agagcaggcc
    25441 cagcgttttc acgaagctgg cgcgaagaat gttgtcatca ccttgggaga aaaaggatgt
    25501 cttttctatg gagagggggc gaagttcacg gcgggtgttt atcccaccac gtttgtcagt
    25561 ggtgcggggg ccggagatgc gtttgatgcg ggattcattg cgggattact gtggggcggg
    25621 tctcccgagg attgtgtccg gtggggcagc gctctggggg caagctgtgt ccgggcgatt
    25681 ggaacaacag aaggtgtttt cacgcgggaa gaggccgtgg cttttatggc ttcacacgaa
    25741 cttcctatct caaaactgaa atgaattgag gtggatttcg tgcaaccgaa ggtacttccc
    25801 ggaaagagat aggagtgagt gatatgcgga tcggcgtttt gacgagtggt ggtgatgcgc
    25861 cggggatgaa tgcctgtatt agggccgtcg ttcgggctgc catttcgatg ggccacgaag
    25921 ttgtcggtat ttaccgcggt tatgcagggt tactccgcga agagttctac aaggatgagc
    25981 gcggtcatcc aatgatgaat ttgcggagtg tttctgatat ttcacggcgt gggggaacga
    26041 tcctccatac tagccgttgt aaggagttct acacggatga cggattacgc aaagcagcag
    26101 aggtcctccg gcgtaacaaa attgacgcgt tgatccccat tggtggtgat ggcacgttcc
    26161 gcggggcggt ggctctggca aagtattggg acggacaaat tgtcggctgc cctggcacaa
    26221 ttgataacga tctgattggt tcggactata cgattggctt tgcgaccgcc gttcacacgg
    26281 ccgttgaggc cgttgacaaa cttcgcgaca cggcagacgc ccatgaaaga atgttcctgg
    26341 tcgaagtcat ggggcggcat agtggctaca tcgcccttta tacggcgatc gccgcagcag
    26401 ccgagattgc ttgtattccc gaaacaccga cggacaccgg ggagattgtc cggctcctcc
    26461 acgatttgaa gttgcggggg aaaacatccg tcatgatcat tgtggccgag ggtgatgagc
    26521 gcggaggggc agccgtcctt caaaaagctc tctgcgaggt gaaatgcccc tatgaggtca
    26581 gagtccttgc cctgggacat ctacagcgcg gtgggagccc cgtccctcaa gaccgtatcc
    26641 tcgcctcgcg acttggagat ttcgccgttc acgcgattct ccaaggaaag tcgggcgtga
    26701 tggctgggga acaaaaaggt gagcttgtct tgactccgct cgaggaaact ttcgccgcgc
    26761 atcggccggt gcccgaagcg tacgtcaaac ttctggaaac ccttgccact tagagactat
    26821 cccacaacct cccctctccc gttcggtacg agaggcgccg ggagtgaggg gggctcaaat
    26881 cgctgccggt tttctcgttc gactccgact atcgtttgcc ctcaccccaa ccctctccca
    26941 gagggagagg gagctctcgg ataggctgtt agagatttcg gctttcaggc ataaacgaga
    27001 accgaccccc tcgcctggaa atggtctcta ttgcgaggtt ccctaatgga actttattac
    27061 gaggctccct aatgggacag agcagcgccg tcgccggttg gattagtttc cagcccggac
    27121 aatgggaaac tgaacgattg tcgcgctgta cggtgcgacc tcgaacgaat ccgcatggga
    27181 aacctcgccg cgactgcgca gctcaatacg agggggttta cccgggtcgt tgtaatccat
    27241 cgggtcattt ccggccacgt cccagacttt accactgcca ccgacggtag cccctgccac
    27301 gcgcaatgtg cagagttgtt tttcgccggt cggattcacg atcgccacag cgagcgtttt
    27361 gttgtctttc agaagggctg cctgtgcatc gagcagcgga ttttgtgacg ccgtcgcaac
    27421 cggaatttgg ccgaagtacc gtcgatagag caccaacatc aatccggtcg tttcaaacgc
    27481 ggcggccgtt ttggtggtct tgatcgctcc aatgacgtta acggtctggg catagtttgc
    27541 cattgcgtaa acgtcggtat tgcgagcgaa ctcgttaagg gccttggcga tgccgaggcc
    27601 atcccggacg aagtaccgcg tcccaagctc cccgaagacg tgttcgccgt accaatagtt
    27661 ccattcatca agggccacgg ggatttcacg cccgccgaga atgtcacggc gataacgccg
    27721 atgggcatcg gcaattcgcc ggacctgcgc ggccgcttgg ttcacgtgct cgatcaggtc
    27781 ttttttggcc ccaacataaa aatgctcact catcaggtcg atgaaatccg cacagtggcg
    27841 aaacattcct tctgtccatg gtcccacatt gcccacggcc acgaccttga tgtgcgggtc
    27901 cttttcccgc atggccctca caaaggcatt gtgtttctcg acatactgtt ccaacggcat
    27961 atgccccaat tgccagttac catacatctc atttccaatg ccccaatatc gcacgccata
    28021 gggtt
//
LOCUS       DAOSUT010000036        27979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1119_length_27979_cov_9.433892, whole genome shotgun sequence.
ACCESSION   DAOSUT010000036 DAOSUT010000000
VERSION     DAOSUT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27979
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1119_length_27979_cov_9.433892"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1309)
                     /locus_tag="PLD05_04955"
     CDS             complement(239..1309)
                     /locus_tag="PLD05_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HOP76816.1"
                     /translation="MEAYRKQPHPPIDILEGNVLDSRLPELCEFYEGLGCPFLSRHPA
                     WLRVLQRGLHHRPVCLVAGEQTSAPLAAILPLCLVKSRLFGAFLVGLPYLNLGGPLGA
                     ATLSGHLIDGAADLADRWNVRYLELRHEEPVSHPALTVTVQSKVHMRLPLPESRDALW
                     QSFSPKVRNQIRKAEKLGLQVHWGRHEQLGEFYRVFARNMRDLGTPVFPKALFGAILD
                     EFPQAEIIVVRYHNRTLAGGLLLHGSGVTEIPSASSLRPWNWTNANMLLYWSALSRTI
                     ERGQQMFDFGRSTIDSPTYRFKKQWGAVPYPAHWQYYVRQGKVGEMRPDSPRFRALIQ
                     IWRRLPVWLTRWIGPWIVRGIP"
     gene            complement(1499..1738)
                     /locus_tag="PLD05_04960"
     CDS             complement(1499..1738)
                     /locus_tag="PLD05_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76817.1"
                     /translation="MSQWGAGLPEGEYLVSVTKFEERPVAEEEEEGPYNPALANMEPP
                     PPKSLIPMKYAQPEQSGLKASVGKSGGTFDFDLTD"
     gene            2380..3396
                     /locus_tag="PLD05_04965"
     CDS             2380..3396
                     /locus_tag="PLD05_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOP76818.1"
                     /translation="MKVKQADRDSWLAKCARQGSGKLHLVRYINGGYQVRVVEKICLA
                     GKDTMTPITMASSIAEHVQRAARRIQEISTLPQVAVNIMRVVEDPRSSAADLKEVMEI
                     DAALSARVIRCVNSSAYGVRHKITNLQQAIAYLGLKQIRNLAITATVADLFRKSESIG
                     RYRRTDLWKHLVASGLCARMLAMRLRFHNFEDMFLAGLLHDIGIILIDQHFHESFVRV
                     IQSIKDGTTLVEIEKGILGFAHTELGEAVGVQWGFPDATRAAIRYHHQTASYRGEHLA
                     LVRCVEVAHLICTLKGMSSIGTNLLRFNPESLSGLGMTANDVKVVADDLDREMAEQMS
                     LLQI"
     gene            3444..5258
                     /locus_tag="PLD05_04970"
     CDS             3444..5258
                     /locus_tag="PLD05_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76819.1"
                     /translation="MELAPKNLSDLLAAVRGPSEPSVSDSPLASQFEHIALLGWFTID
                     EATGTATPSRSLMMHITPGELETLTREILARLGQSNAIACSVGLAAGECWAFACRFVT
                     PFAAVVACVKRTSPEETLPTAEIDIFRWMTLLLATGIIRLENEYWELATRVEHHHAEQ
                     EMLRQAQIESLEETVLEREARLQQQQLRQLWEQKYRESQEANRAKTEFLSNISHELRT
                     PLTAILGYVDLASEAQDHHLIQDYLRRIRRNSDNLLKIVNDILDLAAAEAGRIKPRII
                     PFDPCQVAREAIELITPEVEQRNLKVHLEYFYPIPNLIHSDPVRIRQILLHLLSNAVK
                     FTEKGEVRVEVRWVQVDGGQSEIQYSVIDTGIGFSCDALPELLEPFRQGDNSPHRRFG
                     GNGIGLSIVRRFVQLLGGRLEVASSPGKGSRFTVAFEAPLPPGVSLRMANDDGSQQAV
                     PPSAAEQTAWGMMVPLPHARVLLVEDGLDNRRLITRILERAGVQVTAVENGLQGYQEA
                     VASAAEGRPYDVILMDMQMPVMDGYQATERLRAEGWNRPIIALTAHALPEDRERCLQA
                     GCDDYLAKPIRREALLEAIRRFLPAPQTASEVTSEAAS"
     gene            complement(5264..7831)
                     /locus_tag="PLD05_04975"
     CDS             complement(5264..7831)
                     /locus_tag="PLD05_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOP76820.1"
                     /translation="MNDEYFEGVNSPENQPLPSMQVGSSPENVQESGATEGEINICAL
                     QGQQEYTADKLEHLSDQEHVRKRPSMYIGDTGSRGLHHLVFEVVDNSIDEAMAGYATQ
                     LWVTINPDGSVTVEDNGRGIPVDVHPALGISTLEGVMTLLKFGGKFSKGAYYTSGGLH
                     GVGVTVVNFLSEWCVVEVYRGGAVWRQEYERGKPLGPVRRMGNTHRTGTKTTFKPDPL
                     IFPNTDLNGSIIQRRLQELAFLNKGVKIHFRDLRTNQSETYCYQRGIVEFVEYLNRAS
                     EPLHREVIYLNEEVEGVTIELALQYSSEFTENFHSYVNNICTTEGGTHVSGFRAALTR
                     TFNQYGRDNNLFKDLIPTGDDFREGLTGVLAVRVPHPQFEGQTKTKLGNSEVEGLVAS
                     TVGEFLSKYLEENPKTARAIIDKALLAAEARESARKARQLVRERKSALSGGGLPGKLR
                     DCTSREVDRCELYLVEGDSAGGSAEGGRIREFQAILPLRGKIINAYKAREDKVLASDE
                     IRSIISAIGVGVGDEIDLSKRRYGKIVIMTDADVDGSHIRTLLLTFFYRQMYDLIKAG
                     MVYIAQPPLFRVRRKKEVYYVQTEEEMKNQLLEAGLADAVFEPGDGRTITGEELKKLC
                     RTLAAIENAVLALEKRGISLKEHAARRDPVTGRLPVYHVFLDQNEYWFTSRQELDDFL
                     EKEEMERGETLMVEDERNGEEESKEEQQPARRLHIVELHEVRTINSHLQMLREAGFDI
                     DSLYPIERTGEEKPRYLLRRGDSVQGLEDLRGLLAALRSAGEKGLQITRFKGLGEMNA
                     EELRETTLDPANRTLIQVTMEDAGAADEMFRVLMGEKVLPRREFIEKHALEVQNLDV"
     gene            complement(7907..8209)
                     /locus_tag="PLD05_04980"
     CDS             complement(7907..8209)
                     /locus_tag="PLD05_04980"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOP76821.1"
                     /translation="MPREPHHISQVVADLLARTGVAQPQATRELEAAWREIVGGVLAD
                     VSRPVRIYRARLEVVVAHSTALQELTFRKGEILQQLNQRWPDRRVKDIKFRVGLLK"
     gene            complement(8257..9366)
                     /gene="dnaN"
                     /locus_tag="PLD05_04985"
     CDS             complement(8257..9366)
                     /gene="dnaN"
                     /locus_tag="PLD05_04985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOP76822.1"
                     /translation="MKVVADREKLAHVVQLAGAICPGRGTRPILENLFLDCDDHTGTI
                     MATDLEVGIRAEVPDLTVEVPGKTLLPFRLTASVLREINEDQIRLEADGQKVLLLSSR
                     AEFQFPTPDPLEYPLVPAFQGKAYHSFTARTLREMLVRTEFAVELDSTRFNLGGVFLE
                     LAENSAIAVATDGRRLARQEGAAKQVGKHMPTDHVIIPARAVQLIQRIIGDGEEEVKV
                     AIEEGKIFVITGSSSFYARLIDARFPKWREVFPKDSARISVELPVGELAAALRQAAIV
                     VDSKHPGVWTTFTSGKLVLATRGSEYGESRVEVPISYDGEELTLRLDPRFMLEFLKVL
                     DNDATFTWKVWQPETPVLAETTDGYAYIVMPLISS"
     gene            complement(9672..10352)
                     /locus_tag="PLD05_04990"
     CDS             complement(9672..10352)
                     /locus_tag="PLD05_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOP76823.1"
                     /translation="MSAPRILVVEDDPQIQELVSYNLAREGFVVLPALSAEAALRILE
                     EHPVDLVILDLMLPGMDGMTFCRRIKAQSKTQDIPVIMLTAKSEESDIVAGLEIGADD
                     YIPKPFSPRLLIARIRAVLRRSPRPGDEECYTAGPLTIDRSRHEVRCNDQTIALTPTE
                     FAILDLLASHPGRVLSRAEIAESVHGEPFVTTDRAVDVHIAALRRKLGSHGQLIETVR
                     GIGYRFRD"
     gene            complement(10417..10905)
                     /locus_tag="PLD05_04995"
     CDS             complement(10417..10905)
                     /locus_tag="PLD05_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOP76824.1"
                     /translation="MQSLTNEQRLAMWLNQTGFSLPSPAEPKGLYRPVVVCNNLAYTS
                     GHLPVDASGQMIKGTVGQDLTEEQGRQAAVWAGLGILSSLRATLGNLNDVRRVIKITG
                     LVQSAQGFHGQPAVLNGCSQLFAEVFGPENGVGVRTAFGTPTLPLGAAVEIEAIFELG
                     TE"
     gene            complement(11001..11879)
                     /gene="hisG"
                     /locus_tag="PLD05_05000"
     CDS             complement(11001..11879)
                     /gene="hisG"
                     /locus_tag="PLD05_05000"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOP76825.1"
                     /translation="MSERILRLGLPAGSLQDATADLFRRAGYKITYVNRSYYPVIDDE
                     EIECVLIRAQEMARYVQDGVLDAGLTGYDWIVETNADVIEVAELVFSKVSRRPVRWVL
                     CVPENSPVQSVKDLEGKRIATEAVGITQRYLEKHGVKAKVEFSWGATEVKPPRLADAI
                     VEVTETGSSLRANNLRIVDEVLTSTPRFIANRESYKDPWKRQKIEDIVLMLRGAMAAE
                     GKVSLMMNVPRAKLQDVIAVLPALQKPTVADLSDPNWVAVITVCDESVVRQIIPRLKA
                     AGAQGIVEQPVTKLIE"
     gene            complement(11966..12385)
                     /gene="hisI"
                     /locus_tag="PLD05_05005"
     CDS             complement(11966..12385)
                     /gene="hisI"
                     /locus_tag="PLD05_05005"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOP76826.1"
                     /translation="MSQGGQSNNEDAVLRGPDFSRNNGILPAIAQDAVTGEVLMLAYM
                     NEESFAETLASGRAVYFSRSRNRLWRKGEESGHYQIVKEIYIDCDNDAILLKVEQVGG
                     AACHTGYRSCFYRVLTPDGLRPVGQPVFDPEKVYGKK"
     gene            complement(12685..15606)
                     /locus_tag="PLD05_05010"
     CDS             complement(12685..15606)
                     /locus_tag="PLD05_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76827.1"
                     /translation="MFRYGSAFAVLVCTLGWNIALTELPEPPGGTFRIPAGKTGDGVP
                     VIASWTDDAGPDETFLLVGAGLTDRVYVWGLDPSCPGGREIRPKVQFCTGQMLAATLP
                     ERAYDGVFVVWVENDVGRSRPIVLNAPQPWWCHPRQVQAGGTFRVFGRNLARRPHFGQ
                     SFIYLESENGSGRWLPVVKCEKYQVTAAMPKDVPAGRYRLWFHAGCGGTWGWGGPLVV
                     EVVGPKPEVPVIAFAGGDLQAAIDQLAERGGGILNLPEGVFSLRGTLVIPAGVTVWGQ
                     GRDKTILQAPSDPAIPFARFYPPAWDQGPTGVHTPGDTMVYRVVFPRAGQWAVWMRYA
                     TDMSPWGQPGVSGRMTIALDDQPPVPLENLPNTGSFRTFRWSRAAELVASAGEHRLTW
                     KNVKGGGIHIDAFAFSDDPQWEPVEGQLPESGPHLVVVQGEDVIAFQSKDGSLPGNEK
                     ACVWLAGDGGGLMHLTVLGSVQTNLGIVVRHPQYPQWIRNCRIEDVLVKDCQGKQAEN
                     CGVRLFHAESAVVRGCELWGRSPLFLSGVRQCDFSGNRLVSVTLWGGNAEGYINGRNE
                     TIEECIIEDNICAVPPGAQGGGPTGRRMIWFSTGRGSVSHNWIAGNREDHAIFGGVAG
                     TDQNVGEMILFEACQRIAYWGPIVTANSDNVTLPAKLAKTPEEWLGNVKRESLAYDAE
                     GNETPFYPPEEENEREPVLTQYYVTVLEGPALGQTRRIVARQGTTYHLDRPWQVVPPA
                     GSRIVLSTAFYQNHIVKNRTVDGMTGIQLWISCIENIVSDNEVARQRKPAVYLYGVCT
                     TLASSMPMTWNRGIGPLYFNHIEGTRAEETSCGIFLTSGESAGLPLLFPRCLGNVLRH
                     NGLVKNRTDGVLLIGNRKETKENGYTSVMGTIAEFNLVRDALCGFHVSNAVESTVLRR
                     DHVYFWYPVSLGAKSPVAFQVDDADSTTVLELNSVEGISGGLSQDIIPLLRGGERTDL
                     P"
     gene            complement(15720..16157)
                     /locus_tag="PLD05_05015"
     CDS             complement(15720..16157)
                     /locus_tag="PLD05_05015"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOP76828.1"
                     /translation="MKIIQLSLFLENKPGQLLRICQILAQAQLNLVTLCLADTQQFGI
                     LRLILRDWQKAKELLEAHGFVATTTEVVAIEVEDRPGGMARILEILAERQINIEYLYA
                     FTFKRGDKAVVVFRFDRPDEAIAALQQSGQNVLGPVEIYGDAA"
     gene            complement(16189..17532)
                     /locus_tag="PLD05_05020"
     CDS             complement(16189..17532)
                     /locus_tag="PLD05_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOP76829.1"
                     /translation="MSSNLESWSDGDSPVHPLSAVDFLPRPQLQAMQLYRLQGIVRWT
                     YERVPLFRQRMDERGLKPKDIRELQDIEKLPFSQKADLHACYPTGLFACSMQEIVRLH
                     ASSGTKSKPTVVAYTQKDLDLWASVMARTFLLCGIRRGDIIQNAYGYGLFTGGLGFHS
                     GAEALGATVIPISTGNSDRQIMIMKDFGCTAICCTPSYFLHLIERARDLGVDLKKHPV
                     RVGIFGAEPWTEAMRQRIQEEAGIKAHDCYGLSEIIGPGVAAECPVQQGLHIFEDHFY
                     PEIVDPNTGEVLPEGEEGELVLTTLTKQAMPMIRYRTRDITRLISAPCPCGRTIRRIA
                     RIRRRSDDMLIIRGVNVFPSQIEEALLTVEGTLPHYQIVLTREDNLDQMEVRVEVTPG
                     VFTDTVGAMEQLRQRLIHQIETTVGIRVRVRLVEPQSLQRSEGKAKRVLDLREQQ"
     gene            complement(17545..18045)
                     /locus_tag="PLD05_05025"
     CDS             complement(17545..18045)
                     /locus_tag="PLD05_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOP76830.1"
                     /translation="MSTERVTNIVVAGVGGQGVLRATDVIAETAFLAGYDVKKTEVHG
                     MSQRGGGVVSDVRFGPRVLSPMVPDGEADFLLVLEPTQLEPNRHRLKPDGVIIGCDRI
                     NVASLTHPKTLNVALLGVLSQYLDLSCELWCDALRRCFPPHLYSVNEEAFELGRQADG
                     KACRRE"
     gene            complement(18027..>19061)
                     /locus_tag="PLD05_05030"
     CDS             complement(18027..>19061)
                     /locus_tag="PLD05_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOP76831.1"
                     /translation="IPAYARPAHRRLRQRLKEIAQWNETSGLNLVLTGTSDLGIIASG
                     VAALHAREAAPQTRLLKLGMSYPLPVQTVKEFVRGVKRCVVIEEGDPVIEELCLTHGI
                     PVEGKPEEDRFGELSVSRVRRILNGDCPPPPPPASTRPPQLCPGCPHRMVFEALRDLG
                     CIVAGDIGCYTLGVLPPFEAMDTCTCMGASIGVGLGLRHVLPPDQARRVVSVIGDSTF
                     VHSGLTGIVEMMYNPPPGGHVVLILDNRTTAMTGLQDHPATGRTLSGQPTHELSLEGV
                     CRAIGVEDVTVFEEHSNKESFRALLREKLSQAKPCVIIVRRECLLAMRRRKQESLAAQ
                     QERVTCQPSE"
     assembly_gap    19062..19161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19486..21663)
                     /locus_tag="PLD05_05035"
     CDS             complement(19486..21663)
                     /locus_tag="PLD05_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOP76832.1"
                     /translation="MRRIAVGLFLVALIALAGFPWAGAEDAVVRPDIVLDDFERETYA
                     PWIAEGEAFGPGPAAGTLPRQMEVTGYHGQRLVNSYYNGDGTVGTLTSPPFTLQRHYL
                     AFLIGGGGYHDETYMELVVDGQSVRRAVGPNTAPGGSEELDWAVWDVRELEGKTAVIR
                     IVDRRTGGWGHINVDYIVQTDRPPEQLLQPLETRVTQRYFLLPVAGNGPRTHCRIIHK
                     DNVVRYFDIQIAEDDSQIRFWASIDLQGLEGEIVSWFIRPRAARDLVAKCLVQSNAPR
                     WPPNLYHEAFRPQFHFSPRVGWTNDPNGLVYYDGEYHLFFQHNPFGIPWGNMTWGHAV
                     SRDLVHWEELGDAILPDERGTIYSGSAVVDINNTSGLGKKENPAMCAFYTAAGQHSYQ
                     PCPFTQCMAYSLDRGRTWTKFSGNPVVNFIADGNRDPKVFWHKETQRWIMVLYVRRDA
                     FSIFSSPNLKEWRFETEVPFPTAHECPELFELPVNGNPQDTRWVLWSASGNHMIGRFD
                     GHTFKPESEVLRSEWGKNCYAGQTWNEVPDGRRLFIGWMNSDGSAYPGMPFNQQMTIP
                     REFTLRTTEQGLRLFAWPVKELERIRREKVAVKEIRLVEGSPYELTLGELLDIQMAIK
                     PATAKVVMINVRGISLEYRPQDGVLACLGAQAGDLPKTDVFDLRMLVDRTSLEVFVLG
                     GRYVMSFCWPFDAANRTVRLEAHGGNAEVVSLEGYRLASIWEE"
     gene            complement(21695..23065)
                     /locus_tag="PLD05_05040"
     CDS             complement(21695..23065)
                     /locus_tag="PLD05_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOP76833.1"
                     /translation="MKLNRYLLLCVIVTSLGGLLFGFDTAVISGTEKALQRLFHLSGF
                     WHGFTVASALIGTVIGSLVASYPADRWGRRLTLIVIAILYFVSAVGSALAWDWYSLVF
                     FRFIGGLAVGGASVVAPMYIAEISPPQWRGRLVAIVQFNIVFGILLAFFSNFCVTLFN
                     LGDLEWRWMLGVEAFPAAVFWLLLYLTPQSPRWLVVCGRDQEAVDVFVKLGVPPQEAQ
                     ELVSEIRSGLERTNGNEPLFCPRYMRLVLLALAIAAFNQLSGINAILYYAPRIFQMAG
                     AGKYTAFLQSVAVGGTNLIFTMLAMSVIDRFGRKFLMLVGSIGYILSLSAVATAFYIY
                     GAQFDTFGNIVVLVSLIVFIASHAFGQGAVIWVFISEIFPNTVRAKGQALGSFTHWFM
                     CAVISWTFPVIAEQSGGHAFAFYAAMMVLQLLWVIFIMPETKGIPLEEIEQRLLTKRS
                     RSSQPV"
     gene            23952..25550
                     /locus_tag="PLD05_05045"
     CDS             23952..25550
                     /locus_tag="PLD05_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP76834.1"
                     /translation="MAITEVDLSGRNRARRQDCETPITVTCRVAQDTTSQPSEAKTYL
                     GERQETVANLSQKCQKLSVIVPVYNERWSLAEVIRRVLEAPVTLEKEVIIVDDGSTDG
                     SYEVALELAAQDRRIQVIRHQRNAGKGAALRAGIEAVSGDVVIIQDADFEYDPTDYPT
                     LLEPILQGKADAVYGSRFVGHCRRVLFFWHTLANKFLTLVSNMVNDLNLTDMETGYKA
                     IRTDVLRSLRLTANTFTIEPELTCRLAQCGARIYEVPISYTGRTYDEGKKIRARDGLK
                     ALAAILYYRFIDTQFTHDEALATLQSVKKAKAYHRWLIDIVRPFLGKRILEAGAGIGN
                     LSTLLLNAERLILVDRNPRYCEILRQRFSGRANISVELADLADIELYQHHRLDGLDTV
                     FCANVLEHIADDQRVLSHFYQVLEEGGRCILIVPAGPKLFGSLDEAVGHFRRYSEHDL
                     VQKVRAAGFEVVHCRRFNRLGSLAWFISSRVLRRRTLSPHAMKWFSRLVPLAKLLDWL
                     LPVRGMSLVVVGQKPRQQRSKLAA"
     gene            25630..26466
                     /locus_tag="PLD05_05050"
     CDS             25630..26466
                     /locus_tag="PLD05_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76835.1"
                     /translation="MRLSLGLCVLGGVLVSASGATLVLAALKITGMVPSLGGVALPHR
                     VGWPMLTLLVGMGLLAAGWPSPKSPLLQRWQDFIRSFSQLWNHGNLIWRIVITALIGH
                     LAISLAYFLGAPVDLHETFVERANLQGTLLSIRAGQRYVHWEFLARLWKETIPPRAAV
                     AYRGHWEGPLVAYELYPRRLYLLPEDGRRLAANWHNHRWLAEKTKGKSLADRSTDEFW
                     TQRTIWPSLDLETFIQQRHIEFLLTFDEADEKGCSLQAMPDSLRQEGLKKDSTPPLSS
                     PQ"
     gene            26463..27920
                     /locus_tag="PLD05_05055"
     CDS             26463..27920
                     /locus_tag="PLD05_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76836.1"
                     /translation="MTQVVLTATYFGFLLVIWLAGRMWLRLFSSLFSQCPRDDQPGNA
                     EAFLVGSAMLGLASYLVIAIFGRMPPLGYCLLVAVHCAMALVGWCVARRPAGPQWKRG
                     LKTAFFIWAVILGLYAAQLPLTGYDARAIYGLKAKIVADGANLWGPDFRDPYRLHFGS
                     NYPLLIPILESFLFRFRMILSGGDPWGDVGLPLIFYGFIVAGTLLVTKVTERLAPGWG
                     IFSGVIWAITPMVWRWTEGAGLSGSADLPFAVFLAAGVFHLVEGWSSQDRFSLLLAGI
                     FFGATILIKQEGLIGLGLGTVTIFVDQILWQRSRQKDNTPMVPNRGPSKLASFGVFLV
                     ILIPFLLFHRLVHAEMPQQIYMRSYAAALSWEWLQQITDRPATILSFAMKELINNHWG
                     LAWPALVVALLLKRDRPLSPTVRRIRLFVLLLMAAYTGIFAVTPFPLLYHLHTAYARL
                     MLHALPLAIVVLVEQLAASLDFTHFGHGNTPESTR"
BASE COUNT         6193 a         8300 c         7375 g         6011 t
ORIGIN      
        1 ccacgggcac gcgccccacc gtgcccgtgg ttctttattt tgccaaaaag tggttcgggc
       61 cgaggtgtgg cggcccattt tggagtccgc cccaccgcct cccctctccc gctcggcggg
      121 aaaggggcgg ggggtgaggg tggctcgaat cgctgctggt tttctcgatg gactgcgacg
      181 atggcttacc ctcaccccaa ccctctccca aagggagagg gaattctggg ataggctctc
      241 aaggaatgcc acgcacaatc caggggccaa tccagcgggt cagccacacg ggcaaacgtc
      301 gccagatttg aataagggcg cgaaatctgg gactatcggg acgcatttcg cccaccttgc
      361 cttgccgcac ataatactgc caatgagctg ggtacggcac ggctccccac tgttttttga
      421 agcgatatgt gggactgtcg atggtgctgc ggccaaagtc gaacatctgc tgaccgcgtt
      481 caattgtccg agagagagcc gaccaataaa gcaacatgtt ggcatttgtc cagttccacg
      541 gtcgaaggga acttgcgctg gggatttccg tcacgcccga gccgtgcagt aataagccac
      601 cggcgagtgt gcgattgtga tatcgcacca caataatttc ggcctggggg aattcgtcca
      661 ggatggcgcc aaaaagggcc ttgggaaaga cgggcgtacc cagatcacgc atattccggg
      721 cgaaaacgcg atagaattcg ccaagttgct cgtgtcgtcc ccagtgaacc tgcaggccga
      781 gcttttcagc cttgcgaatc tgatttctga ccttgggcga gaaactttgc cacagggcgt
      841 cacggctttc tgggagggga agtcgcatat ggacctttga ctgcaccgtg acggtcaacg
      901 cgggatggga aaccggttct tcatgtcgca attccagata tcggacattc cagcgatctg
      961 ccaggtcggc agccccgtca atgaggtgcc cactcagcgt agccgccccc aggggtccgc
     1021 caaggttcag gtagggtaat cccaccagaa aggccccgaa taagcgactt ttgacaagac
     1081 agaggggcaa gatggccgcc agaggagcag acgtctgctc gccggcaacc aggcacactg
     1141 gccgatggtg caaaccacgc tgaagaaccc gcaaccacgc cggatgccgt gaaaggaagg
     1201 gacacccgag gccctcataa aactcacaaa gctcaggcaa tcgagaatca aggacgttgc
     1261 cttccaaaat gtcaattggt ggatgaggct gcttccgata tgcttccacc ggatcacgac
     1321 cgagctaacc tagccctgca gttttccacc gcccaaaccg tgaaaatgca tcatcacgca
     1381 ctgccccgca agacgccgcg ccatcggacg aaaaaaatag tcgctcaccc ccttgggcat
     1441 gagcgaccac acttctcatg tgccctacca tttacactac aatcagtcgg gacgcgattc
     1501 aatctgtgag atcaaaatcg aaagtaccac cggacttgcc cacactggcc ttgagacccg
     1561 actgttccgg ctgggcgtat ttcatcggga tcaagctctt gggcggcgga ggttccatat
     1621 tggccaaagc cgggttgtaa gggccttcct cctcctcttc ggcaaccggg cgctcctcga
     1681 atttcgtcac gctcacaagg tattcgcctt cgggcagccc agcaccccac tgcgacatga
     1741 tctgataatt tcccgaggcg tcggtacgag cagcaccgct agcattgccg agggggcttt
     1801 aacaatccat cccacaacct cctctcaccc gttccgcggg ggatgggtcg ggggtgaggg
     1861 tggctcgaat tcctgccggt tttctcaatc gattccgacg attgcttgcc ctcaccccaa
     1921 ccctctcccg gagggagagg gggacttctt gggatagact ctaatcaacg cgcaggcaca
     1981 acagcatgcc cctacgagtg ggtcaaaaac atatacgtga ttacccctca ccttccgggg
     2041 ggcgtaggcc tatagggctc gggacgattg cgattgcggg cttgggcggc taaaaatcgg
     2101 ccaagggatc atcgaaaatc gcccgcatct tgtaacggga gatcaagatg cttgcaatgc
     2161 cgcttcatga cgttgggggg tggcaccgat aaattcggaa atccggtctt gtcgaatagt
     2221 tccactttac ttctcttttc gctaagagcc ggtctttcaa caccgttcaa caatggcctg
     2281 aattcgcctc tgcgatacaa accgtgacct atactttcac agaactccat agcatcctac
     2341 caaaagaaca ccccaaaaac acggaagaaa cccatctgga tgaaggttaa acaagctgac
     2401 cgcgattcgt ggttggccaa gtgtgcccgg caaggatcag gcaaactcca tttggttcgg
     2461 tacatcaatg gtggttacca agttcgtgtt gttgagaaga tatgtcttgc ggggaaggac
     2521 acgatgacac ccataacgat ggcaagctct attgcggagc atgtgcagcg cgcggcgagg
     2581 cgaatccaag agatttcgac actcccgcag gttgctgtca atatcatgcg cgtggttgaa
     2641 gatcctcgtt cgagtgcagc cgacctcaaa gaagtgatgg aaatcgatgc ggccctcagt
     2701 gcccgcgtca tccgttgtgt gaattcgtcg gcctacggtg ttcggcacaa gatcaccaat
     2761 ttgcaacagg ccatcgccta tctcggatta aagcaaattc gtaatctggc gatcacggct
     2821 accgtggcgg atctcttccg caagagtgag agcattggcc ggtatcggcg aaccgatctc
     2881 tggaagcacc tggtcgccag cggtctctgt gctcgcatgc tggccatgcg actacgcttt
     2941 cacaactttg aggacatgtt ccttgccgga ttattgcacg acatagggat tattctcatt
     3001 gatcagcatt ttcacgagtc gtttgttcgc gtcatccagt cgataaagga cggcacgaca
     3061 ctggtggaga tcgagaaggg cattctggga tttgcccata cggagttagg ggaagccgtg
     3121 ggggtgcaat ggggctttcc cgacgcaacc cgcgcagcga ttcgctatca ccatcagacg
     3181 gccagttatc ggggagagca tttggcattg gtgcgctgtg tggaggtggc gcatctcatt
     3241 tgtaccttga aggggatgtc atccatagga acgaatttgt tgcgtttcaa tccggaatct
     3301 ttaagtgggc tgggtatgac ggcaaatgac gtgaaggtcg tggctgacga cctggaccgc
     3361 gaaatggccg agcaaatgtc actcttacaa atctgacggt tgccccagta aggttggcag
     3421 gcgattgaaa aaggcatgat ggtatggaac ttgcgccgaa aaacctcagt gatcttctgg
     3481 ctgccgtgcg gggaccttct gaaccttcgg tgtcagatag tcccttggcc agccagtttg
     3541 agcacattgc cttgcttgga tggttcacca ttgacgaagc cacgggcacc gcgacgccct
     3601 cccgcagcct gatgatgcac atcacgccgg gcgaactcga gaccttgact cgggagattc
     3661 tggcccgctt gggccaatcc aatgcgatag cttgctccgt cggccttgcg gcgggtgaat
     3721 gttgggcctt tgcttgtcgg tttgtcactc cattcgcagc cgttgtcgcc tgcgtgaaac
     3781 ggacgtcccc cgaggagacg ctccccacgg cggagattga cattttccgt tggatgacgc
     3841 ttctcctcgc cacgggaatc attcggttgg aaaatgagta ctgggagttg gccacgcggg
     3901 tggagcacca ccacgccgag caggaaatgc tccgccaggc ccaaatcgag tcactggaag
     3961 agactgtcct tgaacgcgag gcccgcctgc agcagcagca acttcgacaa ctgtgggaac
     4021 aaaagtatcg tgaatcgcag gaagccaatc gggcaaagac agagttcctc tccaatatca
     4081 gccacgaatt acgcacgcca ctgacggcta tcctcggata cgttgacctg gcgagtgaag
     4141 cccaagatca tcatctgatt caagactatc tgaggaggat tcggcgcaat agtgacaatc
     4201 tgttgaagat cgtcaacgat attctggacc tcgctgcggc ggaggccggt cgcatcaagc
     4261 cacgcattat cccctttgat ccctgccagg tcgcgcgcga agccatcgag ttgatcaccc
     4321 cggaagtgga acagcgcaac ctaaaggttc acctggaata cttttacccc atccccaacc
     4381 tgattcattc cgatccagtc cggattcgcc aaattcttct tcacctcctg agcaatgcgg
     4441 tgaagttcac cgaaaagggc gaagtccgcg tcgaagtgcg gtgggtccag gtggacggcg
     4501 gacagagcga gattcaatac agcgttatcg acacgggcat tggattctcc tgcgatgcgc
     4561 taccggagtt actcgagcct ttccgccagg gagacaattc cccgcaccgt cgttttgggg
     4621 gaaacgggat agggctttcg attgtgcgac gtttcgtcca actgctggga ggccgactgg
     4681 aagtggccag cagccccggc aaaggcagcc ggtttaccgt agcttttgag gctcccctgc
     4741 caccgggtgt ttcactgcgt atggcaaacg atgacggttc gcaacaagcg gtgcctcctt
     4801 cggccgcgga gcaaacggcc tgggggatga tggtgcccct tccgcacgct cgggtcttac
     4861 ttgtggaaga tggcttggac aaccgccgcc tcatcacacg catcctggaa cgtgccggag
     4921 tgcaggtgac ggctgtggaa aacggactac aagggtacca agaggccgtt gcatcggcgg
     4981 ccgagggaag gccttacgat gtcatcctca tggacatgca aatgccggtg atggatggct
     5041 accaagctac cgaacgcctc cgcgccgagg gttggaatcg accgatcatc gccctgacgg
     5101 cccacgccct gcccgaggat cgggaacgct gtcttcaagc gggatgcgac gactatttgg
     5161 ctaagccaat ccggcgagag gccctgttag aggccatccg ccgatttctt cctgcccctc
     5221 aaaccgcctc agaagtgaca tccgaggcgg catcctgagg gaattagacg tcgagatttt
     5281 gaacctccag ggcgtgcttc tcaatgaact ctcgccgcgg caagaccttt tctcccataa
     5341 ggactcggaa catctcgtcc gctgcccctg cgtcctccat ggtcacctgg atcaacgtgc
     5401 ggttggcagg gtccaacgtc gtctcgcgga gttcttccgc attcatttct cccaaacctt
     5461 taaaccgggt aatttgtagc cctttttccc cggcagaacg aagggcggct agtaacccac
     5521 ggaggtcttc cagcccctgc accgagtccc cgcggcggag gagatagcgg ggtttttcct
     5581 ctcccgtcct ctcgatggga taaagggaat cgatatcgaa tcccgcctca cgtagcattt
     5641 gcaaatgact gttgatcgtg cggacttcgt gaagctccac gatgtgcaag cgacgggcag
     5701 gttgttgttc ttccttggac tcttcctccc cgttgcgttc atcctcgacc atcagcgtct
     5761 cgccccgctc catttcctct ttttcgagga agtcatcgag ctcttgccgg ctggtgaacc
     5821 agtattcgtt ctggtctaag aacacgtgat agacgggcag acgacccgtg acaggatcgc
     5881 gccgggccgc atgttctttc aagctgatgc ctcgcttttc cagggccaat acggcatttt
     5941 cgatggccgc caacgtgcgg caaagctttt tcaattcctc gcccgtgatc gtgcgaccgt
     6001 cacccggctc aaatactgcg tccgccagac cggcctcaag gagctggttt ttcatctcct
     6061 cttcggtctg cacgtagtac acctctttct tgcgacggac gcggaaaagc ggcggctggg
     6121 caatatacac catccccgcc ttgatcaggt catacatttg ccgatagaaa aacgtcagca
     6181 gaagggtccg gatgtgcgaa ccgtccacgt cggcatcggt catgatcaca atcttgccgt
     6241 accgccgttt gctaagatcg atctcatcgc ccacgccaac gccaatcgcc gaaatgatac
     6301 tgcggatttc gtcgctggcc aggactttgt cctcgcgggc tttataggcg ttgatgattt
     6361 taccccgcaa gggcaagatg gcttggaact cgcggattcg gccaccttcg gcactcccgc
     6421 cggctgaatc gccctccacc aaatacaact cgcaacggtc cacttcgcga ctggtacaat
     6481 cgcggagctt accgggcaag ccaccgccac tcaaagcgct ttttcgctca cggaccaatt
     6541 gcctggcttt tcgagcactt tcgcgggctt ctgctgccaa cagggcctta tcaataatgg
     6601 cccgcgcagt cttgggattc tcttccaaat acttggagag aaactctccc accgtcgagg
     6661 ccaccaggcc ttcgacttca ctattaccga gcttcgtctt tgtttgccct tcaaactgcg
     6721 ggtgtggaac gcgcacagcc aggacacccg tcagcccctc tcgaaaatcg tctcccgtgg
     6781 gaattaggtc cttgaatagg ttgttatccc gcccgtattg attaaacgtg cgggtaaggg
     6841 ccgcgcggaa acccgaaacg tgcgttcccc cttccgtggt gcagatattg ttgacgtaag
     6901 aatgaaagtt ctcggtgaac tccgaagagt attgaagggc cagttcgatg gtaacacctt
     6961 ccacctcttc attgagataa atgacctctc gatgaagtgg ctcgctcgcc cgattcaaat
     7021 attccacaaa ctcgacgatt ccccgctggt aacaatacgt ctcggactga ttggtccgca
     7081 aatcgcggaa gtgaatcttc acccctttgt tgagaaaggc cagttcctga agacgtcgct
     7141 gaatgatact gccattaaga tcggtgttag gaaagatcag cggatcgggc ttgaaggtcg
     7201 ttttggtgcc cgttcggtgg gtgttaccca ttctgcgaac ggggcccaag ggcttgccac
     7261 gttcgtattc ctgtcgccag acggcccccc cgcgataaac ctcgacgaca caccactcgg
     7321 acaagaaatt gacgaccgtc acgccgactc cgtgcagtcc ccctgaggta tagtacgcgc
     7381 ccttgctgaa cttgccgccg aatttgagaa gcgtcatcac accttccagc gtggaaatgc
     7441 cgagggcagg atgcacatcg acggggattc ctcggccgtt atcttcgacc gtcaccgaac
     7501 cgtctgggtt gatggtgacc caaagctgcg tcgcgtaccc ggccatggcc tcgtcaatgg
     7561 agttatccac gacttcaaag acgaggtgat ggaggccgcg cgaaccggtg tccccaatat
     7621 acatgctggg acgctttcgc acgtgctctt ggtcgctaag gtgttccagt ttgtcggcgg
     7681 tatattcctg ctgcccctga agagcacaga tattgatctc cccctcggtt gccccacttt
     7741 cttgcacgtt ttccggtgat gagccaacct gcatcgatgg cagcggttga ttctccggac
     7801 tattcacacc ttcgaaatac tcgtcattca ttgtcacaac tcgattgttt tctcaacgct
     7861 ctcccatccg tgccgaggcc gacaaagacc ccggactcat tctccgtcac ttcaacagcc
     7921 cgacacgaaa cttgatgtct ttcactcgtc ggtcaggcca tcgttggttc agttgctgca
     7981 aaatctcgcc tttgcgaaag gtcaattcct ggagggctgt ggaatgggct accacaacct
     8041 ccagccgtgc ccggtaaatc cgcacgggac gcgacacgtc ggcaaggacg cctcccacaa
     8101 tctcccgcca tgctgcctcg agttccctcg tagcctgagg ctgcgccaca cccgtccttg
     8161 ccagcaagtc agccactacc tggctaatat ggtgtggttc acgcggcaca gcgtcactcc
     8221 ttttcccagc gtcctcaacc cgaccatccc agctcactac gaggaaatca acggcatcac
     8281 gatgtaagcg taaccgtccg ttgtctcggc tagcacggga gtctccggtt gccaaacctt
     8341 ccatgtgaac gtggcatcgt tatcgagcac cttcaggaac tcaagcatga aacgtgggtc
     8401 caaccgcagc gtgagctcct ctccgtcgta actaatgggc acctccaccc gggattcacc
     8461 atactctgaa ccgcgggtcg ctagaacgag cttaccgctg gtgaaggtag tccagacacc
     8521 tgggtgtttg gaatctacga cgattgccgc ctgacgcaga gccgccgcca actcgcccac
     8581 cggcaactcc acagatatcc ttgccgagtc cttgggaaag acctcccgcc acttgggaaa
     8641 acgggcatca atgagccgtg cgtaaaagct cgacgaccct gtaatgacga aaatctttcc
     8701 ttcttcaata gcaaccttga cctcttcttc gccatcgccg atgatccgct ggataagttg
     8761 cacagccctc gccgggatga tgacgtggtc agtgggcata tgcttcccta cctgtttggc
     8821 cgctccctct tgcctggcca ggcgacgccc atcggtcgcc actgcgatgg cactgttctc
     8881 cgccagttct aaaaagaccc cgcctagatt gaatcgcgtg ctgtcaagct ccacagcaaa
     8941 ctcggtccgc accaacatct cgcgcaaagt ccgggccgtg aacgagtggt atgccttgcc
     9001 ttgaaatgca ggcaccagag gatattcgag cggatcaggc gtggggaact gaaactcagc
     9061 gcgagaactc aataacaaca ctttctgtcc gtcggcttcc aaccgaattt gatcttcgtt
     9121 aatttctcga aggacggatg cggtcaaacg gaaaggaagc agcgtcttgc cgggcacctc
     9181 gaccgtcagg tcgggaacct ccgcccgaat tcccacctcc aggtctgttg ccatgatggt
     9241 gcccgtgtga tcatcgcaat cgaggaagag gttctccaaa atcgggcgag taccccggcc
     9301 aggacaaatc gccccagcca attgaacaac gtgggcaagc ttctcacgat cagcgacgac
     9361 cttcatggct gcctcgattc cgaaaaatgc gtccgaaaac ggtgctatgt cattgggaat
     9421 tgcgggtctc aaggaaccag aaaacattat cccacaaaag ccccaaatcc tcgagtctcc
     9481 cccttagcga ggtactggtt tgaacccatg gatgccccgc cgttagtggg ggactgaatg
     9541 cgacttcaat gggacaaagc gtacccgcaa gagcacgtag ctggtacccg acgcgccccc
     9601 gtcgcaccgc attcgcagct tgtgagcgtc gtcctagaga tggctgccac taagaccggg
     9661 cggtcaaatt ctcagtcacg gaagcggtac ccaatcccgc gaactgtctc aatgagctgc
     9721 ccatgcgatc cgagctttcg acgcaaagcc gcgatatgga catcgacggc ccggtcggtc
     9781 gtcacaaacg gttccccgtg gaccgactcg gcaatctccg cccgcgagag gactcgtccc
     9841 ggatgcgaag ccaaaaggtc aagaatagca aactcggtgg gcgtcaaagc aattgtttga
     9901 tcattgcacc gcacctcgtg gcgggaacga tcaatcgtta aaggccccgc cgtgtaacat
     9961 tcctcgtcac caggccgggg ggatcgacgt aacacggcac gaattcgggc aataagtagc
    10021 cgcgggctaa acggcttggg gatataatcg tcggccccaa tttccagccc agccacgatg
    10081 tcgctctctt cactcttggc agtcaacatg atgacgggaa tatcttgggt cttggattgg
    10141 gccttgatac ggcggcaaaa tgtcatgccg tccatgccgg gcaacatgag atcgaggatc
    10201 acaagatcga ccgggtgctc ttccaatatc ctcagggccg cttcggcaga caaagcgggc
    10261 aagacgacaa agccctccct ggccaaattg tagctcacca gctcctgaat ctggggatca
    10321 tcctccacaa ccaggatgcg gggtgcgctc ataattggcc tcgttgacag tggcccacag
    10381 gtgaccaatc ccggacgaac tgtcgtgacc tatgggctat tcggtcccca gttcaaagat
    10441 ggcctctatc tccacagcgg cccccaaggg caacgttggt gtgccaaagg cggtccgcac
    10501 tcctacccca ttttccggtc caaatacctc cgcgaaaagc tgactgcatc cattaaggac
    10561 ggccggttga ccgtgaaatc cctgggccga ctgcaccaag cccgtgattt tgatcacccg
    10621 gcgaacatcg ttcaggtttc ccaatgtggc cctcagtgat gagagaatcc caagccccgc
    10681 ccaaacagcg gcctggcgtc cctgctcctc ggtcaagtcc tgacccaccg tccctttgat
    10741 catttggcct gaggcatcca ccggcaaatg cccggaggtg taggccaagt tattgcacac
    10801 gaccaccgga cggtagagac cctttggctc ggcaggagac ggcagcgaga aacccgtctg
    10861 gttgagccac atcgcgagcc gttgctcatt tgtcagggac tgcacggatt agttcctctc
    10921 gtagtcctca acttgtctcg cagagatatc cttcgatatc cttccgcgat cgcgacctgg
    10981 gcgttggtca agatgccctt ctattctata agcttggtca ccggctgctc cacgatccct
    11041 tgggccccgg cagcctttag cctggggata atctggcgca cgacggattc gtcgcatacc
    11101 gtaatcacag ccacccagtt agggtcggag agatcagcca cagtgggctt ttgcaaggcc
    11161 ggcaagacag caatgacatc ttgtagcttc gctcgtggta cgttcatcat gaggctgacc
    11221 ttaccttcgg ccgccatggc accgcgaagc atgagaacga tatcctcgat cttctgccgt
    11281 ttccacgggt ctttgtagga ctcccgatta gcaataaacc gcggggtgct cgtcaagacc
    11341 tcatccacga tccgtagatt attcgctcgc aaggagcttc ccgtttccgt gacttccacg
    11401 atggcatcgg ccaagcgggg cggtttgacc tctgtagcac cccaactaaa ctccaccttg
    11461 gcctttacgc catgtttctc caaatagcgc tgggtaattc ccacggcctc ggtggcgatg
    11521 cgcttgcctt ccaggtcttt aaccgactgg acgggcgaat tctccggcac acacaacacc
    11581 cagcggactg ggcgacggct cactttcgag aagacaagtt ccgcaacttc gatcacgtcc
    11641 gcgttcgtct caacaatcca atcgtacccg gtcaaaccgg catccagaac accatcttgg
    11701 acgtagcgcg ccatctcctg agcgcgaatc aggacgcact cgatctcctc atcatcgatc
    11761 acgggatagt aactgcggtt gacataagtg attttgtaac cggcacgacg gaacaagtcg
    11821 gcggtggcgt cttgaagact tcccgccggc aaaccaagac gcaagattct ttctgacatt
    11881 cctaatctga cctcgtaact acgaaactca attttggtaa cccaccaaga agttattcga
    11941 ttcccagcag ggtacgatga tcgtcctact ttttcccata cactttttcc ggatcaaaca
    12001 cgggctgacc gacaggccta agcccatccg gcgtgagaac acggtagaaa caggagcggt
    12061 aacccgtgtg acaggccgcc cctcccacct gttcaacttt caaaaggatc gcgtcattgt
    12121 cgcagtcgat gtagatttcc ttgacaatct gataatggcc actttcttct cccttccgcc
    12181 agaggcgatt tcgactgcga ctaaaatata cggcccgacc agatgccagc gtttcggcaa
    12241 agctctcctc gttcatatag gcgagcatga ggacttcccc cgtaacagcg tcctgcgcga
    12301 tcgctggcaa gataccgtta ttccgcgaaa aatcggggcc ccggagcacc gcatcctcgt
    12361 tattcgattg accgccttgg gacatcacca caattccttt tttctgacaa ccacaattgt
    12421 catttcagtc gttcagcatc tggcagaagt cggcttcccg cctgtcccga tattttgtcc
    12481 ccccgtggac gttgaaacca tccattttaa ctgagtccgg tcaaaatccc actgggaacg
    12541 cgaaaagagg caaaggcgtt actcacccac ccggatgggg cattcccgct cgaattatgc
    12601 attcggcttg ggaatggcca atccgtttga ccgccgcttt gccagggcga atacgccaaa
    12661 tgccgaatag catcttgagg gctttcacgg taaatcggtt cgctctcccc ctcttagcag
    12721 ggggataatg tcttggctta atcctcccga aatcccttcc acggaattga gctccaagac
    12781 cgttgtagaa tctgcgtcat ccacctgaaa tgccacgggg gactttgccc ctagcgagac
    12841 gggataccag aaatagacgt gatcgcgcct gaggaccgta ctttccaccg cgttgctgac
    12901 atggaaaccg caaagggcat cgcggacaag gttgaattct gcaatggtgc ccatgacgga
    12961 agtatatcca ttttccttcg tttcctttcg gttacctatc aacagaacgc catccgttcg
    13021 gtttttcaca agtccgttgt gccttaggac gttacccagg catcggggaa agagtagcgg
    13081 cagtccggcg gactcgccgc tggtaagaaa gatcccacag ctcgtttcct cggcccgggt
    13141 cccctcgatg tggttgaaat agagagggcc aattccgcga ttccacgtca tgggcatgga
    13201 ggacgccaac gttgtacaca cgccatacaa ataaacggct ggttttcgct gccgcgccac
    13261 ttcgttatcg gaaacgatat tctcaatgca ggaaatccaa agctgaatgc ccgtcatacc
    13321 gtccaccgtg cgatttttaa caatgtgatt ttgataaaag gccgtggaga gaacaatgcg
    13381 gctccctgcc ggtgggacaa cttgccaggg acggtccagg tgatacgtcg tgccctgccg
    13441 agccacgatg cggcgcgtct ggcccaatgc cggaccctca agcaccgtca catagtattg
    13501 ggtcagcacg ggctcgcgtt cgttctcttc ctccggagga tagaaaggcg tttcattgcc
    13561 ttcggcatca taggccagac tctcgcgttt gacatttccc agccattcct cgggcgtctt
    13621 ggcaagcttg gcggggagcg taacgttatc gctgttcgct gtgacgatcg gtccccaata
    13681 ggcaattcgt tgacatgcct cgaagaggat catttcgccg acgttctgat cggttcccgc
    13741 gacaccgcca aagatggcgt gatcttcacg attcccggca atccaattgt gcgagaccga
    13801 tccccgcccg gtggaaaacc agatcatgcg ccgcccagtg ggaccaccac cctgggcccc
    13861 aggcgggaca gcacaaatat tgtcctcaat gatgcactcc tcgatcgtct cgtttcggcc
    13921 gttgatatag ccctcggcgt ttccacccca aagggtcaca ctaactaagc gattaccact
    13981 gaaatcacat tgccgaacgc cagaaagaaa gagaggtgat cgtccccaca gctcgcatcc
    14041 ccgtaccacg gccgattccg cgtggaacaa tcgcacaccg cagttctcgg cctgttttcc
    14101 ctggcaatcc ttaaccagga cgtcttcgat ccggcaatta cgaatccatt ggggatactg
    14161 cggatgccgg acaacgattc cgagattcgt ttgcacgctc cccaggacgg tgagatgcat
    14221 gagtccccca ccgtcacctg cgagccagac acacgccttt tcattccccg ggagacttcc
    14281 gtctttggac tgaaacgcga tcacgtcttc accttgcacc acgaccagat ggggaccgct
    14341 ctcgggaagc tgaccctcaa cgggttccca ctggggatcg tcggaaaagg caaacgcatc
    14401 gatgtggata ccaccaccct taacattctt ccacgtcaag cgatgttccc ccgccgaggc
    14461 taccaactcc gctgcccgac tccagcgaaa ggtcctgaaa ctgcccgtgt tgggcaaatt
    14521 ttccagtgga acaggcggtt ggtcgtcaag tgcgatggtc atcctgccgg aaacaccggg
    14581 ttgaccccat ggcgacatgt cggtggcgta ccgcatccaa acggcccatt gccccgctcg
    14641 tggaaaaacc acacgataga ccatcgtgtc acccggcgtg tgaacacctg tgggcccttg
    14701 atcccatgcc ggaggataga accgtgcgaa ggggatggcc ggatccgatg gggcctgaag
    14761 aatggtcttg tcgcgtcctt gaccccaaac cgtgaccccg gccgggatga ccaatgtccc
    14821 acgcagtgag aagacccctt cgggcaagtt caagattcca cccccgcgct cggccaattg
    14881 gtcgatcgcc gcctgcaagt cgccgccagc gaacgcaatg accgggacct ctggtttagg
    14941 tcccacaacc tccacgacca gcgggccacc ccagccccaa gttcccccgc aaccggcatg
    15001 aaaccacaat cgatagcgcc ctgccggaac atccttcggc atggcggccg tcacttggta
    15061 tttctcgcat ttcaccaccg gcaaccaccg tcctgagccg ttttctgatt cgaggtaaat
    15121 aaaactctga ccgaagtgcg gacggcgggc aagattgcgt ccaaagacgc ggaaggtccc
    15181 tccggcctga acctgccgtg ggtgacacca ccagggctgc ggcgcgttga ggacgatcgg
    15241 tcgggatcgc ccgacatcgt tttccaccca cacgacaaag acgccgtcgt aagcccgttc
    15301 cggcagcgtc gccgccaaca tctggccggt gcagaattgg acttttggtc gaatttctcg
    15361 gccaccgggg caagacgggt caagccccca aacataaacg cgatcagtca agcccgcgcc
    15421 gacgaggaga aatgtttcat ctggcccagc atcgtccgtc caagaggcaa tcacgggcac
    15481 accgtcccct gtctttcccg caggaatccg aaaggtcccc ccaggcggtt cgggcaactc
    15541 ggtcaaggcg atgttccatc ccaacgtgca gaccaacacc gcaaacgcgc taccatagcg
    15601 gaacatcgaa ggtttcctcc acctccagcg aaaaccagaa ctggccttcc ctgtggatac
    15661 ttccggttgg gaaggattct ctcgcaggtt tctaatggca aggaagattt ccctaggctt
    15721 caggccgcgt cgccataaat ctctaccggt ccaaggacat tctgtcccga ctgctggagc
    15781 gcggcaatag cctcatctgg gcgatcaaaa cgaaatacca ccacggcctt gtcgccccgt
    15841 ttgaaggtaa acgcgtatag gtactcgatg ttgatctgac gctcggcgag gatttccaaa
    15901 atacgggcca tgccgccggg gcgatcctcg acttcaatgg caacaacctc tgtcgttgtc
    15961 gcgacaaaac cgtgtgcctc gaggagttcc ttcgcctttt gccaatcgcg aaggatcagc
    16021 cgtaagatac caaactgctg agtatcggct aagcacagcg tgaccagatt gagctgggcc
    16081 tgggcgagga tttggcaaat gcggagaagt tgtcctggct tattttccag gaataacgag
    16141 agttggataa ttttcatgac gtcgctcctt caattgcgtc acccggcgtc attgttgctc
    16201 tctcaaatcg agcacccgct tggccttccc ctcgcttcgc tgtagggact gcggctcaac
    16261 caggcgcaca cgtacccgaa tacccacagt ggtttcaatc tggtgaatca gtcgctgccg
    16321 cagttgttcc atcgcaccca cggtatcggt aaagacccct ggtgttactt ccacacggac
    16381 ttccatctgg tcgagattat cttcccgcgt tagcacaatc tgataatgcg gcagtgttcc
    16441 ctcaaccgtc aaaagggcct cttcaatttg tgacggaaag acgttgacgc ctcgaatgat
    16501 gagcatgtca tcactacggc gtcgaatccg ggcgatacgg cggatcgtgc gaccacacgg
    16561 gcatggcgcc gaaatcagcc gggtaatatc ccgggtacga taacgaatca taggcatggc
    16621 ctgcttggtc agggtggtca aaaccaattc gccttcttct ccttcgggaa ggacttcccc
    16681 cgtattagga tcaacgatct ccggatagaa atgatcctca aaaatgtgca agccctgctg
    16741 aacgggacat tcagcggcca cgcccggccc aatgatctcc gaaagtccat aacagtcatg
    16801 ggccttgatg cccgcttcct cttggattct ttgccgcatg gcttccgtcc aaggctctgc
    16861 cccaaagatc cccacgcgca cagggtgctt tttcaaatca acgccgagat cgcgggcacg
    16921 ttcaatcagg tggaggaagt aactcggcgt acaacagatg gcggtgcaac cgaagtcttt
    16981 catgatcatg atttgccggt ccgagttccc cgttgaaata ggaatgacgg tggcccccaa
    17041 ggcctcggct ccagaatgga aacccaagcc tcccgtgaat aacccgtacc cgtaggcatt
    17101 ttgaatgatg tcaccgcggc gaatgccaca aagcaagaac gtccgggcca tgacacttgc
    17161 ccacaagtcc aggtcctttt gtgtataagc caccacagtg ggcttgcttt tggtgccact
    17221 cgaggcgtga agccggacaa tttcctgcat cgaacaggca aataacccgg tcggataaca
    17281 tgcgtggagg tctgcctttt ggctaaaagg tagcttctcg atgtcctgaa gctcacgaat
    17341 atctttaggc ttcaggccac gttcgtccat cctttgacgg aacaggggta cgcgttcgta
    17401 cgtccagcgg acaatcccct gtaacctgta caattgcatc gcctgtaatt ggggccgggg
    17461 caagaaatca acagcactga ggggatgcac cgggctatcg ccgtcactcc aggattccag
    17521 gttcgaactc atcgtcatgg ctcttcattc ccttcggcaa gccttcccat cggcttgtcg
    17581 tcccaattca aatgcctcct cgttgacgga atacaaatgg ggtggaaagc acctgcgcag
    17641 ggcgtcacac cacagttcgc acgacagatc gaggtactga ctgagaacac ccagcagggc
    17701 cacattcaag gttttgggat gggttaacga tgcaacgttg atccgatcgc agccgatgat
    17761 cacgccatct ggctttaggc gatgccgatt cggttccagt tgcgtcggct ccaaaaccag
    17821 caagaagtcg gcctctccat ccgggaccat cgggctgagc acgcgggggc cgaaacggac
    17881 atcgctcacg acacccccac ctcgttgact catgccgtgg acctcggttt ttttgacgtc
    17941 gtaacccgca agaaaggctg tctcggcaat tacatccgtt gcccgcagga ccccctggcc
    18001 gccgactccg gccactacaa tgttggtcac tcgctcggtt gacatgtcac acgctcctgc
    18061 tgagccgcca gagattcctg ttttcgtcgc ctcatggcca ggaggcattc acgtctcaca
    18121 atgatgacgc acggtttggc ctgtgacagt ttctcccgca gaagtgcccg aaaactttcc
    18181 ttattggagt gctcttcaaa aacggtgacg tcctccaccc cgatcgcccg acagacgcct
    18241 tccaaactca gctcatgcgt cggctgcccg ctcaaggtcc ttcccgttgc cggatggtcc
    18301 tggagcccgg tcatggcggt ggttctgttg tccagaatca agacaacatg gcctcccggc
    18361 ggcgggttat acatcatttc cacaatgccg gttaacccgc tgtgcacaaa cgtgctatcg
    18421 ccaattacac taaccacgcg acgcgcttga tcaggcggaa gcacgtgccg cagtcccagc
    18481 cctacaccga tgctggcccc catacaggtg caggtatcca ttgcttcaaa gggaggtaaa
    18541 acgcccaggg tgtagcaacc gatgtcgccg gccacaatgc aacccaggtc gcggagggcc
    18601 tcaaagacca tccgatgcgg acagccggga cacaattgcg gtggtcgggt cgaagcaggt
    18661 ggaggcgggg gcggacagtc accattcaaa atacggcgga cacgggaaac actgagttcg
    18721 ccaaagcgat cctcctcggg cttgccctcc acaggaatac catgagtcag gcacaattct
    18781 tcaatgacgg gatcaccctc ttcgatgacc acgcaacgtt taacgcccct cacgaactcc
    18841 ttgaccgttt gaaccggcag ggggtaactc atcccgagct tgagaagccg ggtttgaggt
    18901 gctgcctccc tggcatggag cgccgccacc ccagaggcaa tgattcctag gtcgcttgtg
    18961 ccggtaagga ccagattcag gccgcttgtc tcattccatt gggcaatttc cttgagccgt
    19021 tggcgaagcc gacgatgagc cggccgtgcg tacgcgggga tnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn ncagaattgt atggacaagc gatcgtccgt tggtatcatg
    19201 aaagttggca tgtgacacat tgaaaggaac gttgggaacc ccacgggata cagtaaacgg
    19261 cctcttctct gttccggccc cgtcgtttcg ccttaaccgc gagcggcggg gttttttgtt
    19321 ttgcccacca ttgagtgcgc caaccttggg tgagacccgc gcggctgccg aaacctttgt
    19381 gtgtcatccc cacccggcac gcggcgcgtc atttagctac tgtccgcaaa tccaacgcgc
    19441 agtgctctca ccttttgccg gtgaaaaccc gttggcaact cggggttatt cttcccaaat
    19501 ggacgccaac cggtagcctt ccaaagaaac gacttctgca ttgccgccgt gagcttctaa
    19561 tcgtaccgtc ctgtttgccg catcaaaggg ccaacaaaac gacatgacat accgcccacc
    19621 tagcacaaat acctccagcg atgtgcgatc cacaagcatg cggagatcaa atacatcggt
    19681 ttttggaaga tcgcccgcct gggcaccaag acacgccaga acaccatctt gaggacgata
    19741 ttccagggaa atacctcgca cattgatcat gacgactttt gccgtcgcgg gtttgatagc
    19801 catctggatg tcgagcaatt cgcccagggt caactcgtag ggcgatccct caacgaggcg
    19861 aatttccttc accgcgacct tttcccggcg gattcgttcc aattccttga caggccacgc
    19921 aaagagccgt aagccctgtt cggtggtgcg gagcgtgaat tcccgcggaa tggtcatctg
    19981 ttgattaaac ggcattccgg gataggcgga gccgtcggaa ttcatccagc cgataaaaag
    20041 gcgtcgccca tcggggacct cattccacgt ttgcccggcg tagcaattct tcccccattc
    20101 gcttcgcaac acctcgcttt ccggtttgaa tgtgtggcca tcaaagcgac cgatcatgtg
    20161 attacccgac gccgaccaca acacccagcg ggtgtcttga ggatttccat tgacaggcag
    20221 ttcgaacagt tcggggcact catgggccgt ggggaacggg acttccgttt cgaaccgcca
    20281 ctctttcagg ttaggtgaag aaaagataga aaatgcgtca cggcgaacgt acaagaccat
    20341 aatccatcgt tgggtctctt tatgccaaaa aacctttgga tcacgattgc cgtcggcaat
    20401 gaagtttacc accgggttgc ccgaaaactt tgtccatgtt cgtcctcggt ccagcgaata
    20461 agccatgcac tgagtaaacg gacacggctg gtaagaatgc tggcccgctg ccgtgtaaaa
    20521 cgcgcacatg gccgggtttt cttttttgcc cagacccgaa gtgttgttga tatccacgac
    20581 cgccgaaccg gagtagatgg tgccgcgctc atccgggagg atcgcatcac ccagttcttc
    20641 ccagtgaacg aggtcgcgac tcacagcgtg tccccacgtc atgttacccc aggggattcc
    20701 gaaaggattg tgctggaaaa agaggtgata ctcaccatcg taatacacca aaccgttggg
    20761 gtcgttggtc cagcctaccc gcggcgaaaa atggaactgt ggtcgaaacg cttcgtgata
    20821 gagattgggc ggccaccgcg gtgcgttgct ttgcaccagg cattttgcca ccaggtctcg
    20881 agcggcacgc ggtcggataa accacgagac gatttcgcct tcgagtcctt gaagatcaat
    20941 actggcccaa aagcggattt gagagtcatc ttcggcgatc tggatatcga agtaacgcac
    21001 gacattgtcc ttgtggatga ttcggcaatg cgtccggggg ccattcccgg ccacaggcag
    21061 aaggaaatat cgctgcgtca ctcgtgtctc tagcggctgg aggagttgct caggcggtcg
    21121 atctgtctgc acgatgtagt ccacgttgat atgcccccaa ccgcccgtgc gtctatccac
    21181 gatacggatg accgcggttt ttccttccag ttcgcggacg tcccaaacgg cccaatcgag
    21241 ctcttccgat ccaccgggag cggtgttcgg tcccacggcg cggcgaacac tttggccatc
    21301 caccaccagt tccatgtagg tttcatcgtg atatccgcca ccaccgatca ggaaagccag
    21361 atagtgccgt tgcaaggtga aaggtgggct ggtcaacgtg cccaccgtgc cgtcgccgtt
    21421 gtaataagaa ttgaccaagc gttggccatg atagccagtg acctccattt gtcgtggaag
    21481 agtgccggcg gcaggccccg gcccaaacgc ctcaccctca gcaatccacg gtgcgtaggt
    21541 ctcccgctca aagtcatcca gtacgatatc gggccgcacg acggcgtctt ctgcgcctgc
    21601 ccaagggaag ccagctagag cgatgagcgc caccagaaag agacccactg cgatccgcct
    21661 catggccttg cctccatcca aaatggccta ataatcacac gggttgcgaa gatcgtgacc
    21721 gctttgttaa aagtctttgt tcgatctctt ctagggggat gccttttgtt tcgggcatga
    21781 tgaatatgac ccacaacaat tgcaggacca tcatggccgc gtagaacgca aaggcatgcc
    21841 cacccgattg ttcggcaatg acgggaaatg tccatgaaat gactgcgcac ataaaccagt
    21901 gggtaaaact ccccagggcc tgccccttgg cccggactgt gttggggaag atttcgctga
    21961 taaacaccca aatgacggcc ccctgcccaa acgcgtggga ggcgataaac acaattaagc
    22021 tcacgagcac aacgatgttg ccgaatgtgt cgaactgcgc tccatagatg taaaatgctg
    22081 tagccaccgc ggaaaggctc agaatgtagc cgatggagcc aacgagcatg agaaacttcc
    22141 gcccgaagcg gtcgatgacg ctcatggcca acattgtgaa gatgaggttt gttcccccga
    22201 ctgccacaga ctggaggaag gccgtatatt tgcccgcccc cgccatttgg aaaattcgcg
    22261 gcgcgtaata caagatggca ttgatccctg acaactgatt gaaagcggca atcgccaggg
    22321 cgagaaggac aagccgcata taacgggggc aaaacagtgg ctcattgcca ttcgttcgct
    22381 ctagtccgct acgaatttcg ctaaccagct cctgcgcttc ctggggagga acccctagtt
    22441 tgacaaacac atcgacggcc tcttggtctc gcccgcaaac caccaaccag cgagggcttt
    22501 gcggcgtgag gtagagcaac aaccaaaata ctgccgcggg aaaggcctca acgcccagca
    22561 tccaccgcca ttcgaggtct cccaggttga atagcgtcac gcaaaagttc gaaaagaaag
    22621 ccaacaggat accaaacaca atgttgaatt gaacaatagc caccagccgt ccccgccact
    22681 gcggtggaga gatttcggca atatacatgg gagccacgac cgacgcccct cccaccgcca
    22741 gtccaccaat gaaccgaaag aacaccagcg agtaccaatc ccaagccaag gcactcccca
    22801 ctgccgagac aaaatagaga attgcgatga cgatgagggt caaacgccgt ccccatctat
    22861 cggccgggta actagccacc agcgagccga tgaccgtgcc gatgagggca ctggccacgg
    22921 taaagccgtg ccaaaagcca gatagatgaa acaatcgctg aagtgccttc tccgtgccgg
    22981 aaatcaccgc cgtgtcaaag ccgaaaagca acccacccag cgaggtcacg ataacgcaca
    23041 acagaagata gcggttaagt ttcattggtc agctccgcct ggctactcac gaaacggcca
    23101 ttgacgcacg atagcttaca gcctatccga cgccctcccc tctcccgttg gacgggagag
    23161 gggccggggg tgagggtggc tcgaatcgct gccggttttc tccatcgatt ccgacgttcg
    23221 cttgccctca ccccaaccct ctcccggagg gagaggggag actcttcggg atagactcta
    23281 aatacgaaga aacaccgcac gcgtcaccca ccagacaacg caagcccgcg tgtccgtccc
    23341 ctgcgtccag cccgccatgg ctgggtcata attcccccaa agataacagg gtccacccca
    23401 gacatcaacg cgagggccta cccaccgtta cgcattcact gacgaagctc acgaaggccg
    23461 aagaagcctc aagcctacaa attgccatat cctcggaaca cgtgacctta ggtttacaag
    23521 atccctctct ccagctcatc cgccaacaaa tcacctatgt tcgcccgaag catcgtaaag
    23581 ccccctgtgg ttgccccgtt tggcgcaggg agggtcgccg tccccggcgg ccaaatcacc
    23641 gggcaacacg gacgcccgtg ggaaacaacc gatcaacacg gtgatcgaaa ataccacggc
    23701 caagatgcct gtgccacgca cagggcgcag ggcaggcaca ggggcctgcc cctacggttt
    23761 tgttgcggcg cacgtagggg cacccccccg tggtcgcccc gcttggcgca gggagggtcg
    23821 ccgtcgtcgg cgaccaaatc acctttgctc ttgacttttc gctgtttgtc aaatcaaaag
    23881 gatgtcttat aatcaacttc gatgggacgg aacggtggaa atcgtacggt tacagcaagg
    23941 gagggatccc aatggccatc acggaagtcg atctatccgg ccgaaatcgc gccaggcggc
    24001 aagattgtga aacacccata acagtcacat gtcgtgtcgc ccaggacacg acttcgcaac
    24061 cgtcagaggc aaagacctat ttgggagagc ggcaagagac ggttgccaac ttgtcgcaga
    24121 aatgccaaaa actttccgtg attgttcccg tttataatga acgctggtcc ctggctgagg
    24181 tcattcgccg cgttttagag gcccctgtaa cacttgagaa agaagtcatc atcgtcgacg
    24241 acggatcgac cgacggttct tacgaagtgg ctctcgaatt ggccgcgcaa gatcgccgaa
    24301 tccaggttat tcgccatcag cgaaacgccg gcaaaggggc agccctgcgg gcaggcatag
    24361 aggccgtctc tggcgacgtc gttatcatcc aggacgccga ttttgaatac gatccgaccg
    24421 attatcccac gctcctggaa ccgattctcc agggaaaggc cgacgccgtt tatggctccc
    24481 gttttgtcgg ccattgtcgc cgcgtccttt tcttttggca caccttggcg aacaagtttc
    24541 taacccttgt ttccaatatg gtcaacgacc tcaacttgac cgacatggag acgggctaca
    24601 aagcgatcag gacggatgtc cttcgcagcc tgcggttgac ggccaacacc tttaccattg
    24661 agcctgagct aacgtgccgt ctcgcccaat gtggggcgag gatttacgaa gtgcctattt
    24721 cctacacagg tcgaacatac gatgagggga aaaagattcg tgctcgagac ggccttaagg
    24781 ccctggccgc catcctctat taccgcttca tcgacaccca gttcacccat gatgaggccc
    24841 tggcgacact tcagtctgta aagaaggcca aggcatatca ccggtggctt attgatatcg
    24901 tccgcccatt tcttgggaag cgaatcctgg aggccggggc cgggatcggc aatttatcaa
    24961 cactccttct caatgccgaa cggttgatcc tggtagatcg gaaccctcgc tattgcgaaa
    25021 tactccggca acgattctcc ggtcgggcga acatctccgt cgagctcgcg gacttggccg
    25081 acattgaact gtatcaacac catcgcctag acgggctaga caccgtcttt tgcgcaaatg
    25141 ttctggagca tattgctgac gatcaacggg tcttgtcgca cttctaccag gttcttgagg
    25201 aaggcggacg gtgcatcttg atcgtacccg ccggaccaaa gctcttcggc tccctggacg
    25261 aggctgtggg gcacttccgc cgatacagcg aacacgattt ggtgcaaaaa gtccgcgcgg
    25321 cggggtttga ggtcgtccac tgccgacggt tcaaccgatt ggggagcctc gcgtggttta
    25381 tttcatccag ggttttgcga cggcgaacct taagccctca cgcgatgaag tggttttccc
    25441 gcctcgttcc cctggccaaa ctgctggatt ggctacttcc cgtacgggga atgtcgctgg
    25501 ttgttgtcgg ccaaaagcca cgtcaacaaa ggtcgaaact ggcggcataa agcagatgcc
    25561 ctatcatggt gaatgttagt ccttgtcatg gcaaacgtaa tgatccacgc tgacaaagga
    25621 caaaagagga tgaggctttc ccttgggcta tgcgtcctcg gtggtgtttt ggtgagtgct
    25681 tccggcgcga cgctggttct cgcggcgctg aaaataacgg gcatggtccc ttcactcggt
    25741 ggtgtcgcgc tgccccatcg cgtgggttgg ccgatgctga ccctcctggt cggaatgggc
    25801 ttgcttgccg ccggctggcc ttcgccgaaa agtcccctac ttcaacgttg gcaagacttc
    25861 attcggtcct tttctcaact atggaatcac gggaatctaa tttggcggat tgtaatcacc
    25921 gctctaatag ggcatttggc gatttccttg gcctattttc tcggagcgcc ggtggactta
    25981 cacgaaactt ttgtggaacg ggcaaatctc cagggaacgc tcctcagcat tcgcgcggga
    26041 cagcgctacg ttcactggga gttcctggca cgcttatgga aggagacaat acctccacgg
    26101 gccgccgtgg cctaccgagg acattgggaa ggaccactcg tcgcttacga gctttacccc
    26161 cgcaggttgt atttgctccc cgaagatggg cgccgcctcg ctgccaattg gcacaaccac
    26221 cgctggctcg ccgagaaaac caagggaaaa tccttggccg atcgatccac ggatgagttt
    26281 tggacccagc ggacaatatg gcccagtctc gacctcgaga cgttcatcca gcagcgccac
    26341 atcgagttcc tgttgacatt cgacgaagca gacgaaaagg gctgctcatt acaagcaatg
    26401 ccggattctt tacgtcagga aggcttgaaa aaggactcga cgccgcccct atcatccccc
    26461 caatgactca agtagttctc acggcaactt atttcggttt tcttctggtc atctggctcg
    26521 ccggccggat gtggttgcga ctattttcct ctcttttttc gcaatgtccc cgcgatgacc
    26581 aacccggtaa cgcagaagca ttcctcgttg gaagtgccat gctagggctg gccagttacc
    26641 tcgtgatcgc catcttcggt cggatgccac cgctgggcta ctgtttactc gtggcggttc
    26701 actgcgcaat ggctctcgtt ggatggtgtg tcgcccgaag gcctgccggt ccccaatgga
    26761 aacgaggttt aaagaccgcg tttttcattt gggcagtcat cctgggcctt tacgcggccc
    26821 aattgccgtt gaccggctac gatgcccggg ccatttatgg gcttaaggcc aaaatagtgg
    26881 cagatggggc caatctttgg ggcccagact tccgcgatcc ctatcggcta cactttggca
    26941 gtaactatcc cctgctaatc cccattctag aaagcttcct ttttcgtttt cgcatgatct
    27001 taagcggtgg cgatccatgg ggcgacgtgg gtctgccgct aatcttctat ggattcatcg
    27061 tggcggggac actactcgtg accaaggtca cagagcggct ggcaccaggt tgggggattt
    27121 tcagcggcgt gatctgggcc ataactccca tggtctggcg atggacggaa ggggcagggc
    27181 tctccggatc ggccgatctt cctttcgccg ttttcttggc cgcaggcgtc ttccatctcg
    27241 ttgagggctg gtcctcacaa gatcgtttct cactactctt ggccgggatc tttttcgggg
    27301 ccacaatctt gatcaaacaa gaaggcctca tcggcctagg acttggaaca gtcacgatct
    27361 ttgtggacca aatcttgtgg caacgatcgc gtcagaagga caacactcct atggtgccca
    27421 atcgtggtcc aagcaaactc gcttcttttg gtgtctttct tgtgatactg attccgtttc
    27481 ttcttttcca ccggctggtc cacgcggaga tgccccagca aatctacatg cgatcgtatg
    27541 ccgccgctct ctcttgggag tggttgcaac aaatcacgga tcgacccgca accattttgt
    27601 cttttgcaat gaaagaactg atcaacaacc attggggact agcctggccg gctttggttg
    27661 tggcactttt gttgaaaagg gaccgccctc tttcgcccac tgtgcggaga atccgcttgt
    27721 ttgtgctcct tctaatggca gcttataccg gcatcttcgc agtgacacct ttccctctac
    27781 tgtatcacct gcacaccgcc tatgcacgat tgatgcttca cgccctgccc ttggccattg
    27841 tggtattggt ggagcaattg gctgccagtc tggactttac ccactttgga cacggcaaca
    27901 cgccggaaag caccaggtga tgcggaaatt cttcgctcgc gcaacagtaa acaaatccgg
    27961 caactacgtg cggcttgaa
//
LOCUS       DAOSUT010000037        27833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1125_length_27833_cov_9.606379, whole genome shotgun sequence.
ACCESSION   DAOSUT010000037 DAOSUT010000000
VERSION     DAOSUT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27833
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_27833_cov_9.606379"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..2069
                     /locus_tag="PLD05_05060"
     CDS             384..2069
                     /locus_tag="PLD05_05060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP76837.1"
                     /translation="MSNEANTQSSSGDAPTIEFAPSIGVADSQLPSEESPALRCGPVK
                     FVTGSGQDWTCEIDAVLRRRLRLALWVLGLGFAVFLPVHYYFGEIATRTGGFLFLGHG
                     SLTLVLVGCAVWLTLARQSPSFTTLRLVEVAAFGLTGVFFGFLSWNSMEKQCLEIGQV
                     RFEGGFWLILIYTYALFIPNSFWRSAIVIGSIALVPIVLLYVGWLFHPELARAMTTEH
                     TVVIPLVFVVAGITALLGVDTIHHLHRQSFEANRLGQYYLKQLLGRGGMGEVYLAEHK
                     LLKRPCVIKLIRPDKAGDPRLIARFEREVRTMARLSHWNTVEIFDYGISAEGVFYYVM
                     EYLPGLDLGQVVAQFGRQHPARIIYILRQVCDALREAHSLGFIHRDIKPANIFLAHRG
                     GVSDVVKVLDFGLVKPISHRGESLDLTLDGAVTGSPLYMSPEQVLGEDNLDARTDIYS
                     LGAVAYYLLTGRPPFSGEKPIKVMLAHVYETVTPPEKVVPDLPPDFCQVVLRCLEKDP
                     DRRFANVDELDHALLSCADADGWDREKAAAWWRRHLPSDAQPKLEVSQVSAPV"
     gene            2293..3717
                     /gene="leuC"
                     /locus_tag="PLD05_05065"
     CDS             2293..3717
                     /gene="leuC"
                     /locus_tag="PLD05_05065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOP76838.1"
                     /translation="MPNTPTGPKTLFEKIWDAHVVHTDEGGQSLLYIDLHLVHEVTSP
                     QAFEGLRLAGRKVRRPERTVATVDHNVPTTDRSLPIADPISKQQIDTLRRNCQEFGIR
                     FYDIHDIHQGIVHVIGPELGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLA
                     TQTLLQAKPKTFEVRVKGQLAQGVTAKDLILYLIGQLTTRGGVGYCIEYTGEAIRRLS
                     MEERMTVCNMTIEAGARAGLIAPDETTFSYLRGRPFAPQGEAFEKAVAEWRQLATDPG
                     ARYDRVEVFDAGVVEPQVTWGTNPAQVTGVTGVVPDPAQFLDAVERKAAENALQYMGL
                     EPGTPITEIRIDKVFIGSCTNGRIEDLRAAAAVVKGYRVADGVQALIVPGSGLVKRQA
                     EQEGLDVIFKNAGFEWREPGCSMCLAMNPDRLEPGERCAATTNRNFEGRQGRGGRTHL
                     VSPAMAAAAAVVGHFVDVRNWDYR"
     gene            3763..4356
                     /gene="leuD"
                     /locus_tag="PLD05_05070"
     CDS             3763..4356
                     /gene="leuD"
                     /locus_tag="PLD05_05070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOP76839.1"
                     /translation="MKPFVRHTGLVVPLDRSDVDTDQIIPKQFLKRIERTGFGQFLFY
                     DWRFREDGTPNPEFILNAPQYQGATILLARRNFGCGSSREHAPWALADYGFRALIAPS
                     FADIFYNNCLKNGLLPVRLDENLVDDLFRRCREKPHYQLTIDLEACLVTDDFGFRASF
                     DIDPFRRKCLLEGLDDIALTLQHEDKILAYEKARGIV"
     gene            4612..5187
                     /locus_tag="PLD05_05075"
     CDS             4612..5187
                     /locus_tag="PLD05_05075"
                     /inference="COORDINATES: protein motif:HMM:NF022273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HOP76840.1"
                     /translation="MVLWDAVRIFFATLWDKRVAEGVRSLLRGGQVAEPQRPSVEPDR
                     PVTVAPPKPPLAKPREKLTKSEALILLETLQREARFVDFIKEDLSGYSDAQIGAAVRD
                     IHRDCAAVLERLFAIRPVVTATEGADFELPHDFDSGRFRIVGNVVGSGPYHGKLVHHG
                     WEATTCQLPLWQGSESAVRIIAPAEVEVVGQ"
     gene            5315..7126
                     /locus_tag="PLD05_05080"
     CDS             5315..7126
                     /locus_tag="PLD05_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOP76841.1"
                     /translation="MKPTFVIGIDLGTTNLVLSFTPYDADEAHPQILEIPQLVAPGTI
                     ERRTTLPSFLYLARDHEIASRSLDLPWASDQTFAVGEFARRQAAESPERTISAAKSWL
                     CYSRVDRRQPILPWGAPPEVPRISPVTAAQRYLEHLIAAWHHQFPDAPIRQQRVVLTV
                     PASFDAAARELTREAALAAGLPEDLILLEEPQAALYAWLADTGPRWRKLLKVGDQVLV
                     CDVGGGTTDFTLVAVAERDGELALERLAVGNHLLVGGDNMDLAMSHLVSQYFAEKGIQ
                     LNPWQTVSLWHACRVAKETLLGENPPEKYPVTILGRGSRLIGGTVSVEVDRESIAQFL
                     LNGFFPICDVTDRPERRRASGFREIGLPFESDTAVTRHLAAFLQSHGSSKGQPARPTH
                     VLLNGGVFKAKPFQERLLQVLTHWFSESPSPQLLSGVHDLDHGVAKGAAYYGWAKDHG
                     GVRIRGGTARAYYVGIETAGPAVPGIPRPLRLLCVVPRGMEEGTATDVPSDEIGLIVG
                     EKVIFRFFSSSTRKDDRPGDVHESWSPDEIEETDSLEGCLPADEGVQGDYVPVRLESR
                     VNELGMLELWCLSTTDDKKWKLEFSVREDADAEVSPT"
     gene            7165..10113
                     /locus_tag="PLD05_05085"
     CDS             7165..10113
                     /locus_tag="PLD05_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOP76842.1"
                     /translation="MANIDEIETKPPRFVVGMDLGTTNSAVCYVDTAESPWQIRTLSI
                     PQLVAPGVVESREILPLFHYEPAPGEFDPALLRLPFGPAQPQYIVGVLARDHGANVPG
                     RLISSAKSWLCHSGVDRTAPLLPWHGAPDVTRLSPVEVSARYLGHIVQVWNHQFPNYP
                     LAEQDFVLTIPASFDEIARQLTVKAAALAGLNRITLIEEPQAAFYSWIYRHAQSWPQV
                     IKPGQKILVCDIGGGTTDLTLIRVRRAEDGTFRFHRVAVGEHLILGGDNLDLALAHHI
                     ESKLGEDEKLDPQTWSVLVRKCQRLKEELLGPQAPERLSVSIPGRGTRLIGGARRVEL
                     ERAEVVRLLVDGFFPIVELNDKPNVRRIGFQEFGLPYAPDPAVTRYLAAFLTAHRYVA
                     LDETNPAYDPARPDLILFNGGLFFSEVIRQRVLDVVTYWFAKEDKSWQPIAIENPRLD
                     LAVAEGAAYYGMVRRGEGVRISAGLARSYYLGVDVPHPNVVAASVARQGKARFSWTTE
                     EEANVSGASPSAAQIEEASTRECLGFGMCVIPSGVEPGQEVSLKEKTFRLRVGEPVEF
                     PVYYSGTRLTDQPGDLIPVYREELTPLPPIRTVLKTGKRSSASELDVTIHAQLSEIGT
                     LELWCAQVEGRGTWRLEFDVRSAVQTDLRPHEGLGESEGVLDEETASRCLALIDHVFS
                     AEGRDPPENLLKRMSQVTEMNRKDWPPTFLRRIWQRLIENEVGRRRSAAHEARWLNLT
                     GFALRPGFGLAADDWRVSETWRILHGKLFHPTPACRAEWWVLWRRIAGGLTAGHQQAL
                     ANPLLASFRSFHKQQTTGTGNSDFPYSSHEATEILRLLGSLELIAPHWKVELGDMVLD
                     LLPKKKLEHLHEAMLWLLARLGARVPMYGPLNCLVPADVAVRWLDHLVKIDPPSPLMP
                     FVVMQLARLTQDRYRDLPQKARDRALKYLAEHNASDHLLRLVREGGHLETDEQTQLFG
                     ESLPKGLRVA"
     gene            10342..>11073
                     /locus_tag="PLD05_05090"
     CDS             10342..>11073
                     /locus_tag="PLD05_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOP76843.1"
                     /translation="MSRCLGIGTIVIAVLLTGIACGQEIVAHRGASADAPENTLAAFR
                     LAWEQGADAIEGDFRLTADGHVVCLHDADTKRVTNDQVNWKVSQVTLEQLRTLDVGRW
                     KGETFAGERIPTLAEVLAIIPPEKKFFLEIKGGPELVEPIERVLRSSGVDLKQVVVIA
                     FDQDTVLEVRKRLPELKVYWLVSYKRDKATGRWSPTVEEVFQTLARIKPHGLDTEGNP
                     EVVNRAFVERLRREGYEFHVWTIDDP"
     assembly_gap    11074..11173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11174..13024
                     /locus_tag="PLD05_05095"
     CDS             <11174..13024
                     /locus_tag="PLD05_05095"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOP76844.1"
                     /translation="PLKGFRSYKKNAYGLIERRYIKWSDIEVCAGDTVERIIAYTNKI
                     TEIDGKRFEDLNVKLVPRVYLDWNGTPGRQYWPADLQTFDYDSPAFQERLRKLVEKLG
                     QAWDNDPRIFAVQMGLIGYFGEHHNPAPTPQQRRLLVEAFQKAFKNKPILVRHTDPEF
                     MEAGFGIYYDTFGNLDREPPHGSKKQFPWQATHVYPDIWKLAPIEGEVEYNWQNTRPS
                     AKPDETFGRTPNETMKVPAFRRYMIDKIRRYHASYLGWISGYDDTDPEVVAGASELQK
                     VFGYRFVLDSVSYPLRIEPGDELVVKFTVRNTGSAPFYLDWPVAVGLLNPVTKRPVWF
                     APLKGVDLRRWLPGEDWDSGAFAYRLPPRVYSEEGHAAVPEHIAPGRYIVALAILDRQ
                     GGMLPSARFAVENYFRGGWHSFGYIGVGQSPGEPTLEGISFDSPAFDDSLHYVVPEEL
                     RRVRTPPIPAIKPVTPWTPDPSRELINPWRYWTLDSGDTFLDKEVHHDGPVEGPAGRR
                     VIQVKGAFGPWATLNYEFPPNTPLTEGSYRLSFLVRGTPKESIEIELVENGRTVAKVG
                     QVQLEAQWRSHTIEFKLAKKLDEKGSLRFRLPRNVEGSIELTDTHLKRLE"
     assembly_gap    13292..13391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13392..15269
                     /locus_tag="PLD05_05100"
     CDS             <13392..15269
                     /locus_tag="PLD05_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76845.1"
                     /translation="IRIGTLNELAEGVRRPFIERRGIKFNIPLDVRTPSYSDNGDSFQ
                     ANIPEVWSTAFWHEFLDEMARHRYNVLTLWNLHPFPSLVRVPEFPEIALDDVWRTRLR
                     LDDTFSMSGRDMVRPEMLQDVEIVRRMTIDEKIAFWRHVMQYAQDRGIEVYWFTWNIF
                     VWGTDGKYGITADRNNPVTIRYFRASVRELVRTYPLLAGIGITAGENMGRDGEGLTKE
                     QWLWQTYGEGIRDALAEQPGRQFRLIHRFHMTKLGEILQEFRNYPGPFDFSFKYSIAH
                     MYSIPNPPFIKSALEGMPPGMKTWLTVRNDDIYSFRWGDPDYARAYILNMPGPDRLAG
                     FYMGPDGYCWGREAIDVDGELPRQLVMKKQWYSFLLWGRLSYDPQLSNDHFRKILAAR
                     FPEVPSDQLFAAWVEASQVFPYITRFFWGDIDLRWFPEACTRHPAPKGFYTVKDFILG
                     ETMPGSGVLSIREWLSQGEKAGETAGVTPLQIAETLETHSREALRLLKELKPKEKPRR
                     ELQRTLGDLEAMAYLGQYYSAKIRGAVALAEFDRDGKPEQQEAAVKHLEIALAHWKHY
                     ADIAHSQYKPQLLNRIGFVNLHEIAGYVEKDIAIAKQWQPGTLKDIPPGPRTADVPFR
                     P"
     gene            complement(16197..17216)
                     /locus_tag="PLD05_05105"
     CDS             complement(16197..17216)
                     /locus_tag="PLD05_05105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP76846.1"
                     /translation="MPADHADRLSRRDFVCQASAFAGAAVLGSVVSSEQARSVESPSV
                     GPLPRRTLGRTGESITIFTLGTAPCGNARPVDEIAEVVNAAIDLGVTSIDTAPAYVRA
                     EEGVGKVMKTRRKEVFLATKVPADSVEEGQRSLEKSLRLLQTDYVDLLYYHSVGNRKV
                     EGALDPDGAFTWLVKQKQAGKCRFIGISGHNLPGRFAQFLETGEVDVLLVVLSYVDRH
                     TYNFEEKVLPIARKHNVGIIAMKVLGGADPKKGGYGNPQSVGRLASARPDRVETAIRY
                     ALSLPGVTSVNLGINSVEQLKQDLVYFQRFEPLSEAEMDQLLEEGRELAAAWGAHFGP
                     VVEEA"
     gene            17595..17975
                     /locus_tag="PLD05_05110"
     CDS             17595..17975
                     /locus_tag="PLD05_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOP76847.1"
                     /translation="MTKQNQIYYCEQCGNMVEVLEAGQGQLVCCEVPMKRIAENVGDA
                     AQEKHVPAVTREGKSLHIKVGSVIHPMTPEHLIEWVEMFQEDRCIRVFLKPGQAPELR
                     VPAAQAPATVRAYCNLHGLWKVDV"
     gene            complement(18248..19978)
                     /locus_tag="PLD05_05115"
     CDS             complement(18248..19978)
                     /locus_tag="PLD05_05115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOP76848.1"
                     /translation="MDRFEDKPREYQANSFASSQLQESAHRHTWMPPEDFSSSLQGIS
                     PATFRVLIVDDDALILRLLERWLTKAGYQVSQAKDAETALQMVREEPPHILITDWEMP
                     GMKGPDLCREMRLLRLPHYVYVLIVTSHNESANLITALSQGADDFLAKPIREAELMAR
                     LGAAIRILSAQRQLQEMAQRDALTGLLTQRSFHDFLEKEFEVARRTKRPLSCAMVDLD
                     FFKRINDIHGHQAGDYVLRTTAQLLLASCRASDIVCRYGGEEFCVLLPDATEENAVKW
                     AERFRAELGATPFTIGKTTLSLTCSIGISQVYDDTLTPEQLVDQADQALLCAKRAGRD
                     RLVRYHEISLSEEVNLSDKHRQGDLFEGILARHVMSPIVAPLRGDETVDQAAEFFLRS
                     RISSAPVVDETGRLIGMMSEKDLLSAMTSVHGWSRPISDLMRPHVITYPLDAPVRIIY
                     EFLCRVSIRRVIITDNGVPIGAISRGSLLRWFRNLVLSKAYAKEDAGVPYNSGDTRLA
                     ALREALAAISLEAQQLRQTLDKNAPDVVAHVVGAASRIQELATDMLAFANIARDEAIS
                     PALGAAALWD"
     gene            complement(20143..23841)
                     /gene="smc"
                     /locus_tag="PLD05_05120"
     CDS             complement(20143..23841)
                     /gene="smc"
                     /locus_tag="PLD05_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOP76849.1"
                     /translation="MPLKSVLPGEAGFVVHVMLKALEIYGFKSFADRTRFEFRPGITA
                     IVGPNGSGKSNVVDAIKWVLGEQSIKNLRGKEMTDVIFNGSATRRPMNFAEITITLDN
                     SRGILPLDSPEVHITRRLYRSGESEYLINRQTCRLRDIRDLLAGTGLATQAYSVIEQG
                     KIDQLLQATPLERRVVFEEAAGISRFKSRKLEAQRRLERLEQNLLRLNDIVEEVEGRL
                     RVVRSQAGKAQRYQRYADRLKELRTHLARMDWHRLNRELARLEEEGSQLNRDQETLVE
                     EAEQLERRLKEIESQDSHLAQQLTDLQSQLAADRETIAALEADLEHGRARDRELEKEL
                     GRLRKVLAELNLRAGELCSHRQSTAEALRAAEEQYRDLMRQVAEGERSLTNDLEALDR
                     RRTEYEERRAEHWEVLQKVLAADNRLQQLQREHENLQASYERNSRESEEIKRIVGETE
                     QRLDELKNHSADLLQRLQTTLQDLHRLSEERDRCRQELQQIREEWSALHQRRTAAAER
                     ARLLREWEEQLEGLSLGVKDFLHRVQTSESHLGDSVCGLVADLLQTELQVALLVDVAL
                     GDAAEWIVLRNSMPWLDQLRRGSAALNGRVCLLCLDQFRKKADGQSMPTDISSLGQEP
                     GVLGLAEDFVQVEEEFLPVRHFLLGKTWFVESLDTALALASRYADLRFVTLAGELITS
                     EGLIVAGQQSSGLGLVSRRSELRNLTRSIAQWDQQLQQYQEAIATSEHQLKTVEQRIA
                     NLEEQRRSLSQELKDCEQRQQELQRMLQEYLGRCGALEGEIAAAAEGVERIVQQQQQA
                     LLEKTQWEEKAAELSIAITQLGEEIAQLEANVQDRHGVLTALKIELAKSEERLAALQN
                     QLRRQEEAEAERLKEVTEIKNRLAETEKRLMASQRELLSLEARLAELYGRKDELSRVW
                     QETAEARRSLQKERQKHQQQLQDVRRRLRQIEERQHKLELDLNSVRHEKSTLIQRVEE
                     DYGISLNDLDHPCSPEESQDRTEIQREIDDLKRKIHNLGNVNLEALEELQQLEERYNG
                     LSSQCRDLTSAKNSLERIIERINADSRRLFAATLQTVSEHFGTLFRELFGGGQASIEL
                     EPGRDILESGIEIVARPPGKEPRNISLLSGGEKSLTCVALLLAIFRSRPSPFCVLDEV
                     DAALDEANIDRFTKVLRDFLSITQFVIVTHSKRTAICADTLYGVTMQESGVSKRVSVR
                     LEDLSDQGEILSAGSVQSFSTSPEEKAA"
     gene            complement(24098..24502)
                     /locus_tag="PLD05_05125"
     CDS             complement(24098..24502)
                     /locus_tag="PLD05_05125"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOP76850.1"
                     /translation="MYRVTREIDFCYGHRLLQYEGKCRYLHGHNGRLLVTFETDSLDK
                     LGMVLDFADIKRVLQGWIDENLDHRMILHREDPVVPVLQSMGEPLYLMAENPTAENIA
                     RHIFDYVRAAGLPVVETQLWETPRCSAIYRPA"
     gene            complement(24584..>25015)
                     /locus_tag="PLD05_05130"
     CDS             complement(24584..>25015)
                     /locus_tag="PLD05_05130"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOP76851.1"
                     /translation="QKVPSADSPDEAVFLPGRNLLLLTVAGLWCRQHGVTRLAIGVLD
                     SNPFADAAEGFFTKLEDLLNVYGPPEVRIIRPLAGMSKTAIMHWERTLPLEETFSCIA
                     PIDGRHCGRCNKCAERQRAFQEAGIPDRTLYAFSKIEHGHS"
     assembly_gap    25016..25115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25263..25832
                     /locus_tag="PLD05_05135"
     CDS             25263..25832
                     /locus_tag="PLD05_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP76852.1"
                     /translation="MVLSEIDRHLLERCLERKPRAWEDFVDRFLGLVIHVINHTARSR
                     SLRMTPELRDDLCSEVFLTLLKDDFAVLRRFRGQCSLASYLCVVVRRVVVKALLQRMG
                     DSHLQESSSVPPPKGLTTSSEEERINNREEAERLLSLLEGNEATVVRMFHLEGRSYQE
                     ISEATGIPTNSIGPILSRARSKLRRITTA"
     gene            complement(25900..27447)
                     /locus_tag="PLD05_05140"
     CDS             complement(25900..27447)
                     /locus_tag="PLD05_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76853.1"
                     /translation="MTVWRSLVGLLVGVTWLSVSVVSGADYYCPSCATCQANVQFFGH
                     YPTRWRPWPGESRPDIHFPQSIGAEPTKRPAGESRPELPKETLQPIIPTPRRPFPEPS
                     PADTMPEPQGEAYPPPSAPMPPAVPSLNTATPPSPPPVPPAEAPPQMPPTSVPPATSL
                     PLSPSPQPQPETGNLPGLMPSTPPPAQPMPPAGAAPNPMPAGSPGTSGVIISGPQLSG
                     MPYSGQSVSSSTYSAPQISPMPPTPPWVIAGTNDLASRPSGKTTGTSGVAQSSKPGPL
                     TIVNPTVVASTPTTPMDRPLPQNKLEISRSESVPDIAVAPWLPSVATSNYTPLPGKTP
                     SISTHVAQGNPSTATFATPEPGPAVTVWAEDSAESRVTPAGFTVPSAKDATVANSPTA
                     PVASVTANDVVPALNGYCPVELVENEAWVKGQARFAVEYQGKTYFCGGATQKRKFQTN
                     PERYVPVCNGRDPVLLVDQGVLSEGKIEHCAVYDGRLYMFSSTASLARFRLNPQHYAR
                     TALQFQP"
     gene            complement(27508..>27833)
                     /locus_tag="PLD05_05145"
     CDS             complement(27508..>27833)
                     /locus_tag="PLD05_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76854.1"
                     /translation="AQVGGISQVSSKRIWIARPSPAGTGCEQILPVDWNAITRGGNTA
                     TNYQVLPGDRIFIAHDDMIAFHAWIDKTVAPVERLFGFTGLGTSTVRSLQTMGRGYNR
                     TRSGW"
BASE COUNT         5885 a         7550 c         7844 g         6254 t
ORIGIN      
        1 gggtgagggc aagcgatcgt tggagtcgac cgagaaaacg ccgccgattc gagctacgtt
       61 cagccctgct ccctctcccg cccaacgggc gaggggaggt tgtgagatag actcttggaa
      121 cgttccgcgg cttcacgtac tgggggtagg aaggatagaa agactgggaa aaacaggtgg
      181 ggcgtatccg gccaaccggg gccatagtga aaggacgcct tgcctcgcgg ggtcagacgc
      241 tggcgaagca gctttaagat gtcccgccgc caggccaccg tataatatgg tgatttgagc
      301 tgctcggaca cgggttttgc cgtgcgggcg ttttggggca cgatgaactt ccaagtttgc
      361 cccgtcagta gggaatccat aacgtgtcga acgaggccaa tacgcaatcg tcatcgggcg
      421 atgcccccac tatcgaattt gctccttcga ttggggtggc tgacagccaa cttccatctg
      481 aagagtcacc agcgctccgc tgtgggccgg ttaagtttgt gaccggctct ggtcaggatt
      541 ggacctgtga aatcgacgcc gtcctgcgga ggcgactgag acttgccctt tgggttttgg
      601 gcttgggctt tgctgtgttc ttgccggtcc attattactt cggtgagatc gctacccgca
      661 cgggcgggtt cctctttcta ggccatggct cgctcacgct cgtcctggta ggttgtgccg
      721 tgtggttgac actagcacga cagtcgccga gtttcacgac actacgtctt gtggaagtgg
      781 ctgcctttgg cctaacgggc gtcttttttg ggttcctatc atggaactcc atggaaaaac
      841 aatgcctcga aattgggcag gtgaggtttg aaggtgggtt ttggcttatt ctcatctaca
      901 cgtacgcgtt gttcattccg aactctttct ggcgatcggc cattgtcatc gggtcgattg
      961 cccttgtgcc gattgtcttg ctgtacgtgg gctggctctt tcatcccgaa ttggcccggg
     1021 ccatgacgac ggagcacacc gttgtcatcc cgctcgtttt cgtagttgcg ggaatcacgg
     1081 ccctcctcgg agttgacacc atccatcacc tgcatcgcca gtcatttgag gccaatcgat
     1141 tggggcagta ttacctcaaa caactcctgg gaagaggtgg aatgggtgaa gtctacttgg
     1201 cagagcataa gctcctcaag cggccatgcg tgatcaaact tatccgcccg gataaggccg
     1261 gcgatccccg actcatcgca cgatttgagc gcgaagtgcg gacgatggca cggttgtccc
     1321 actggaatac cgtcgagatt ttcgactacg gtatcagtgc ggaaggggtt ttctactacg
     1381 ttatggagta ccttccgggt ttggatcttg gacaagttgt ggcgcaattc ggtcggcagc
     1441 atccggcacg gattatttat atcttgcggc aggtgtgcga tgcccttcgc gaggctcata
     1501 gtttggggtt tattcaccgt gacatcaaac ctgccaacat ctttttggca caccgaggcg
     1561 gggtctccga tgtggtgaag gtcctggatt ttggattggt gaaaccgatt tcccatcgcg
     1621 gtgagtccct cgatctaaca ctcgatggtg ccgtgacggg ctcgccgctt tatatgtcgc
     1681 cagaacaggt tctaggggaa gacaatcttg atgcccggac ggatatctat tccctagggg
     1741 cggtggccta ctatttgttg acaggtcggc caccgttttc gggagagaaa ccgatcaagg
     1801 tcatgttggc ccatgtctat gagactgtca cgccgccgga gaaagtggtg ccggacctgc
     1861 cgcctgattt ctgccaggta gtccttcgct gtctggaaaa ggatcccgat cggcgattcg
     1921 caaacgtgga tgaactcgac catgccctcc tgagctgtgc tgacgcggac ggttgggatc
     1981 gcgaaaaggc cgccgcgtgg tggagacgtc atctgccaag cgatgcccag cccaagctgg
     2041 aggtttcgca agtgtcggcc cccgtttgag tggaccagcg acctccccca ctcatccgcg
     2101 attagtcccc ccagcgccaa ttgatccaat ggccagttgc cctgtacgct catgggtgac
     2161 gtccttcaag gggtctgatg gcctacgcta gggggaaacc taaccaaatc gagcacgtca
     2221 gcgtaaaata tacaatcttt gtgacctcgc gaactgtcac ttgatgcata ggcttcatgt
     2281 ggatatcgca ctatgccgaa cacaccgact ggaccgaaga cgctcttcga gaagatttgg
     2341 gatgcccacg tcgttcacac cgatgagggg ggccagtccc ttctctatat tgacctccat
     2401 cttgttcacg aggtgaccag tccccaagcc tttgaggggt tacggctggc cgggcggaaa
     2461 gttcggcgtc cggagcggac cgtggccacc gttgaccata atgtgcccac tacagatagg
     2521 tcactcccca tcgcggaccc catctcgaag caacaaattg atacgcttcg gcgaaattgt
     2581 caagaattcg gcatccgctt ttatgacatt cacgacatcc atcagggaat tgtccacgtc
     2641 attggtccag agttgggact gacacaaccg gggatgacaa ttgtttgtgg ggacagccac
     2701 acagcgaccc acggggcgtt tggagcgctg gcgtttggga tcggcaccag cgaggtggag
     2761 cacgttttgg ccacgcaaac gctccttcag gccaagccta agacgttcga ggtccgggtg
     2821 aaaggacagt tggcccaggg tgtgacggca aaggatctga tcctctattt gatcggtcaa
     2881 ctcacgaccc gcggcggcgt gggctattgc atcgaataca ctggggaagc gattcgtcgc
     2941 cttagcatgg aagagcggat gacggtttgc aatatgacga tcgaggctgg ggctcgggct
     3001 ggccttatcg ccccggatga gacgacgttt tcctatcttc gggggcgtcc cttcgctccg
     3061 cagggcgagg ccttcgaaaa agccgtggct gagtggcgtc aattggcgac cgatcctggc
     3121 gcccgctatg accgtgtaga agtcttcgac gcaggggtcg tcgaaccaca ggtcacttgg
     3181 ggcaccaatc ctgcccaagt gacgggagtc acgggtgttg tgcccgatcc ggcccagttt
     3241 ttggacgcgg tggaacgcaa ggccgcggag aatgccctcc agtatatggg gctagaacct
     3301 gggacgccga tcaccgaaat ccgtattgac aaggtcttca tcggctcatg cacgaacgga
     3361 cgcattgagg atttgcgggc agcggcggct gtggtcaaag gttatcgggt ggccgacggt
     3421 gtccaggccc tcattgtccc aggcagtggc ctagtgaagc gccaagcgga gcaagaggga
     3481 ttggatgtca ttttcaagaa cgcgggtttt gaatggcgcg agcccggatg tagcatgtgt
     3541 ctggccatga atccagaccg tcttgaaccg ggcgagcggt gtgcggccac aaccaatcgc
     3601 aatttcgaag gccgacaagg acgcggtggg cggacgcatc ttgtgtctcc cgccatggcc
     3661 gcggcggctg ccgtggttgg gcattttgtc gatgtccgca attgggatta ccggtgaggt
     3721 cccccggcgc tcgggtcgca ttttttgtgg gagttaactc acatgaaacc attcgtacgg
     3781 catacgggat tggtagtgcc cctcgatcgg tcagacgttg atacggacca aattattccc
     3841 aagcagttcc tcaagcgaat tgagcgaact gggttcggac agttcttgtt ctacgactgg
     3901 cgcttccggg aggatggaac ccccaatccg gaattcatcc ttaatgcccc acagtatcaa
     3961 ggtgccacca tcttgttggc gcggcggaat tttggctgcg ggtccagtcg tgaacacgcc
     4021 ccctgggccc tggcggacta tggctttcgg gcactgattg cgcccagttt tgccgacatt
     4081 ttctacaaca attgcctcaa gaacgggcta ttgcccgtgc ggctggacga aaatcttgtc
     4141 gatgatttgt ttcgacgctg ccgagaaaag ccgcattatc agttgacgat tgacttggaa
     4201 gcctgcctgg taacggacga ctttggtttc cgcgcgagct tcgatatcga tccttttcgg
     4261 cggaaatgtc tgctcgaggg cctggacgat attgccctga cgttacagca tgaggacaaa
     4321 atcctcgctt atgaaaaggc ccgggggatc gtgtgatccc ctccgctggg caaaccgaac
     4381 tatgatgccc acgagaggac aaaaaacaga ttcgccgggc caagtggctt tcccaaaagg
     4441 cccgagtgtc ccacgactcc ggtctttcgg tagaattgac ggtagcgtca aaggttggcc
     4501 gaagaaccgc tggccaatga gggcttgccg tagcgaggtg ttgcgttggg aaaaaccgtc
     4561 cggcaacccc ttacatgggc aaatttccgc tagtaagtac aggagcctcc gatggttttg
     4621 tgggatgccg tccgaatatt tttcgcgacg ttgtgggata aacgggtggc tgaaggcgtc
     4681 cgctccctcc ttcgtggtgg acaagttgcg gagccgcaac gccccagtgt ggagccagat
     4741 cgccctgtca ccgttgcacc accaaaaccg ccgttggcaa agccccgtga aaaactgacc
     4801 aagagcgaag cgctgatcct cctggaaacc ctgcaacggg aagcccgctt tgtggacttc
     4861 attaaggagg acctcagtgg atactcggat gcccagattg gtgccgctgt ccgggatatc
     4921 caccgagatt gtgccgctgt gttggaacgg ttgtttgcca ttcgccccgt agtaacggcc
     4981 acggaagggg ctgattttga actgccacac gatttcgact cgggccgttt tcgcatcgtg
     5041 ggaaatgttg tcggctctgg accctaccac ggaaaactgg ttcatcacgg ctgggaggcc
     5101 acaacttgtc agttgcctct ttggcaaggt agcgaatctg ccgtcaggat cattgcaccc
     5161 gccgaggtgg aagtcgtcgg ccagtagctc tgtggctggt ccacgttagg taaccgtgcc
     5221 tccgccacga ccgtgtgtga ggtttggttc ggcctaaccg aagagctgtg ccgcttgcaa
     5281 gcgaacaacg tctcaagagg gggagttacg aagaatgaaa ccaacgtttg tcattgggat
     5341 tgacctggga acaactaact tggtgttgag cttcactccg tacgatgcgg atgaggctca
     5401 cccacagatt ctggagatac ctcagcttgt ggcgccgggt accatcgaac ggcggacaac
     5461 gctgccgtcg tttctctatt tggcgcggga ccatgagatt gcgtcgcgga gtctcgacct
     5521 gccctgggcg tccgatcaga cgttcgccgt gggggaattt gctcggcgac aagcggcgga
     5581 gtctcctgaa cggaccatca gtgcggcgaa atcatggttg tgctacagtc gcgtggatcg
     5641 acggcagccc atccttccct ggggggcacc accggaggtc ccccgcatct cgcccgtaac
     5701 ggccgctcag cggtatctgg agcacctcat agcggcttgg caccatcagt ttccggatgc
     5761 tcccattcga cagcagcgag ttgtgttgac agttccggca tcttttgacg ccgcagccag
     5821 agagctgacc cgcgaagcag ccctcgccgc gggattgccg gaggacttga tcctcttgga
     5881 agaaccgcaa gcagcccttt acgcctggct tgccgataca ggtccgcgat ggcggaaact
     5941 gcttaaggtg ggcgatcaag ttttagtgtg tgacgtgggt ggagggacaa cagatttcac
     6001 gctcgtggcc gtggccgaac gggatggtga attagcactg gaaaggctgg ctgttggcaa
     6061 tcatcttctc gtcggtggtg acaacatgga cctggcgatg tcccatctgg tcagccagta
     6121 ctttgccgag aaggggatac aactcaaccc ctggcaaacg gtttcgttgt ggcatgcctg
     6181 ccgtgtcgct aaggagactt tactgggcga gaatcccccg gagaaatacc ctgtcacgat
     6241 cctcggccgg ggcagccgat tgattggcgg gaccgtttcc gtcgaggtgg accgggagag
     6301 tatcgcccaa tttctactca atggtttctt ccccatctgt gatgtgacgg atcgcccgga
     6361 acggaggcgg gcctccggct tccgcgagat cggtcttccc tttgaatcgg ataccgctgt
     6421 gacgcgccat ttggcggcgt ttctccagtc gcatggctcg tccaaaggcc agccggcacg
     6481 accaacgcac gtcttactga atggaggggt cttcaaggcc aaacccttcc aggaacgttt
     6541 gctacaagtc ctcactcact ggttttcgga aagcccatcg ccacaattgc ttagcggggt
     6601 gcatgatttg gatcacggtg tggccaaagg cgccgcttat tacggttggg ccaaggatca
     6661 tgggggcgtc cgcattcgcg gtggaacggc acgtgcctac tacgtgggca tcgaaacggc
     6721 cggcccggca gttcccggta ttccgcgccc cttacgtttg ctctgtgtgg tcccgcgggg
     6781 catggaggaa ggcaccgcga cggatgttcc ctcggatgaa atcggcctta ttgtcggtga
     6841 aaaagtcatc tttcgattct ttagttcttc cacacgaaaa gatgatcggc cgggagacgt
     6901 ccacgaatcg tggtctccgg acgagattga agagacggat tcgcttgagg gttgtctgcc
     6961 cgccgatgaa ggtgtccagg gagattacgt ccccgtgcgg ttggaaagcc gagttaacga
     7021 gttgggaatg cttgagttgt ggtgcttaag cacgacggac gacaagaagt ggaaactcga
     7081 attcagcgtg cgggaagacg cagacgctga agtctcccca acctaatagt cttccactct
     7141 cgatggggtc gatcagaaga tgccatggca aacatagatg agatcgaaac aaaacccccg
     7201 cgcttcgttg tggggatgga cctcggaacg acgaattccg ccgtttgcta cgtcgatacg
     7261 gcggagtctc cttggcaaat tcggacctta tccatccctc aattggtcgc accaggcgtg
     7321 gtggaatcac gggaaatcct gccattgttt cactatgaac cggcgcctgg cgaattcgat
     7381 cctgctctgc tccgtctccc ttttgggccg gcccagcccc aatatatcgt cggtgtcctg
     7441 gcacgggacc acggcgccaa cgtaccgggg cggctcattt cctccgctaa atcgtggttg
     7501 tgccattccg gagtggatcg cacggcaccg cttcttccct ggcacggcgc cccggatgtc
     7561 acacgtttgt cccctgtgga ggtctcggca cgctacctcg gacacattgt gcaggtctgg
     7621 aaccaccaat ttccgaacta tccgttggcg gagcaagatt ttgtccttac cattccagcc
     7681 tcatttgacg agatcgcgcg acagctaacc gtcaaggcgg cggcactggc tggtttgaat
     7741 cggatcacac tcatcgagga acctcaggcg gccttctatt cgtggattta ccgccacgcc
     7801 caaagttggc cgcaagtgat taaacccggc cagaagattc tggtgtgtga catcgggggt
     7861 ggaacaacgg acctgacgct cattcgggta cgtcgcgcgg aggatggcac atttcggttt
     7921 catcgtgtgg cggtgggtga acacttgatc cttggtggtg acaacctcga tctggctttg
     7981 gcgcaccaca tcgagagcaa actcggggaa gacgaaaagc tcgatcccca gacatggtcg
     8041 gtcctggtgc ggaagtgtca gcggctcaaa gaggagttac taggtccgca ggctccggag
     8101 cgattaagtg tcagtatccc cggtcgggga acgcgtctga tcggtggcgc caggcgtgtg
     8161 gaactggaac gcgcggaagt tgtcagattg ttggttgatg gattcttccc tatcgtcgag
     8221 ttgaatgaca agcccaacgt tcgccgtatt ggttttcaag agtttggcct tccttatgca
     8281 cccgatccag ccgtaacacg ttatctagcg gcatttttga ccgctcatcg ctacgtcgct
     8341 ttggatgaga caaaccccgc ctacgatccc gcccgcccag atttgatctt gttcaatggg
     8401 ggcctgttct tctccgaagt aattcgccaa cgggtcctgg acgtggtaac gtattggttc
     8461 gcgaaggagg acaagtcgtg gcagccgatt gcgattgaga acccgcggct tgacctcgcg
     8521 gtggccgagg gagccgccta ctatggcatg gtccgtcgcg gcgaaggcgt gcggatttcg
     8581 gctggtttgg cccgcagcta ttatctcgga gtcgatgtcc cccatccgaa cgtcgtggcg
     8641 gcatcggtcg cccggcaggg aaaggcacgt ttctcgtgga cgacggaaga agaggccaac
     8701 gtgtccggag catcgccatc ggctgcgcag attgaggaag cctccactcg tgaatgtctc
     8761 ggctttggga tgtgtgtcat tccgtcgggc gttgagccgg gtcaagaagt ttctctcaaa
     8821 gaaaagacat ttcgcctccg tgttggcgag ccggtggagt ttcccgtgta ttattcgggc
     8881 acgcgcctca ccgatcagcc gggcgatctt attcccgtgt accgggaaga gctaacacca
     8941 ctgccgccta tcaggaccgt cctgaaaacc ggaaaacggt cctctgccag tgagctcgac
     9001 gtaacaatcc acgcccagct ttcagaaatc ggaacgctgg aattgtggtg cgcccaagtt
     9061 gaaggacgag gaacctggcg gctggagttc gacgtccgtt cggcggtaca gacggatctc
     9121 cgaccgcacg aaggactggg agaaagcgag ggtgtgctgg atgaggagac ggcatctcgt
     9181 tgcttagctt tgattgacca cgtcttttct gcggaaggac gcgatccgcc agagaacctg
     9241 ctcaagcgga tgagccaggt gacggaaatg aaccgcaagg attggccgcc aaccttcctt
     9301 cgtcgcattt ggcaaaggct catcgagaat gaggtgggac ggcggcgaag tgccgcccac
     9361 gaggcacggt ggctcaatct cacgggtttt gctcttcggc ccgggttcgg cctggcggcg
     9421 gatgactggc gggtgtccga gacctggcgc atccttcacg gcaagttgtt ccaccctacc
     9481 cccgcgtgcc gggccgagtg gtgggtcctt tggcgccgca tcgccggtgg gttaaccgcg
     9541 ggccatcaac aggccctggc gaacccactc ttggcctctt tccgatcctt ccacaagcag
     9601 cagaccaccg ggaccggaaa ttcagatttc ccgtattcca gccatgaggc gaccgaaatc
     9661 ctccgtctcc ttgggtcgct cgaattgatc gctccacact ggaaggttga acttggcgac
     9721 atggtcttgg acttgttacc caagaaaaaa ctggaacatc tccacgaggc catgctttgg
     9781 ttgctcgcaa ggcttggtgc aagagttccc atgtatggac cactcaattg ccttgtcccg
     9841 gcagatgtgg ccgtccgatg gctggaccac ctcgtcaaga ttgacccgcc atcgcctctc
     9901 atgccctttg tcgtcatgca gttggcacgc ttgacgcagg atcgataccg agatttgccc
     9961 caaaaagccc gcgatcgtgc cctcaagtat ttggcagagc acaacgcctc agatcattta
    10021 ttgcgactgg tccgcgaagg gggacatttg gagaccgacg agcagacaca acttttcggc
    10081 gaaagcctcc cgaaaggctt acgagtggca taagatcacg cgaacataca ggtttgacca
    10141 gaaaaagact tacttcccgc aaaggacggc aagcagacct aaatggggca aaatggggag
    10201 tgctcaggtt caatcgaaaa tctttgcagg cacatatggc gatcctcttc tggttggaac
    10261 atactaggga cttgtaggaa taagaccagt gagactgtct tcttgtggga aagacaacag
    10321 caaaaaaggg ttaactgcga gatgtctcgt tgcttgggaa ttggaacgat tgtgattgcg
    10381 gttctcttga cgggcatagc gtgtgggcaa gagattgtcg cccatcgcgg ggcttctgcg
    10441 gatgcgcccg agaatacttt ggcggctttt cgactggctt gggaacaggg ggcggacgcg
    10501 attgagggtg attttcggct aacggcggac ggacacgtcg tgtgcctgca cgatgccgat
    10561 acgaagcgcg tgacaaacga ccaggtcaac tggaaagtgt cgcaagtgac gctggaacag
    10621 cttcggacgc tggacgtcgg ccggtggaag ggcgaaacct tcgccgggga gcggattccc
    10681 acgctggcgg aggtcctcgc cattattccg cctgagaaaa aattcttcct cgagattaaa
    10741 ggcggcccgg agcttgtcga gccaattgag cgggttttgc gatcttccgg ggttgatctc
    10801 aagcaggtgg tcgttatcgc gtttgatcag gacactgttc tagaagttcg caagcgactc
    10861 ccggagctta aggtttactg gttggtctca tacaagcggg ataaggccac gggtcgctgg
    10921 agtcccaccg tcgaagaagt cttccaaacg ttggcccgga tcaagcctca cgggttggac
    10981 actgagggca acccggaagt cgtcaatcgg gcgtttgtgg aacgcttgcg ccgtgagggc
    11041 tatgagttcc acgtctggac catcgacgat cctnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnncccctca aaggctttcg ctcctacaag aagaacgcct acgggctaat
    11221 tgagcggaga tatatcaagt ggagtgacat tgaggtgtgc gctggggata cggtggagcg
    11281 catcattgcc tacacgaaca agatcaccga aatcgacggc aagcgattcg aagaccttaa
    11341 cgtcaaactt gtgccgcgcg tttatcttga ttggaacgga actccgggaa ggcagtactg
    11401 gccggccgat cttcagacgt ttgactacga cagtcctgct tttcaggaac ggttaaggaa
    11461 attggtggaa aaactggggc aggcatggga taatgatccg cgtatctttg ccgtacagat
    11521 ggggctaatc gggtactttg gcgagcatca caatcccgcg ccaacccctc agcagcgtcg
    11581 cctcctggta gaggcttttc agaaggcgtt caagaataag cctatcctcg ttcggcatac
    11641 ggacccagag tttatggaag ctgggtttgg tatctactac gacacctttg gcaatctcga
    11701 tcgcgaaccg ccacatggct caaagaagca gttcccttgg caagccaccc acgtttaccc
    11761 tgacatctgg aaactcgctc cgatcgaggg cgaggttgag tacaattggc aaaacactcg
    11821 cccgagtgcg aaaccggacg agaccttcgg acgtacgccg aacgagacaa tgaaagtacc
    11881 agcttttcgg cgatacatga tcgacaagat tcgccgctat cacgcaagtt atctcggctg
    11941 gattagcggg tacgacgaca ccgatccgga ggtggtggcc ggtgcgtcag agcttcaaaa
    12001 ggtctttggc taccggtttg tcctggattc cgtgagctat ccgctcagga tagaacctgg
    12061 cgatgaactt gtcgtcaaat tcactgtacg aaacactgga agcgctccat tttatcttga
    12121 ttggcctgtg gctgtcggac tcttaaaccc ggttacgaaa cgcccggtct ggtttgcccc
    12181 gctgaagggg gtcgatctcc gcagatggct gcccggagaa gactgggata gcggtgcgtt
    12241 cgcttatcgc ttgccgccca gggtctattc cgaggaaggc catgcagcgg taccggagca
    12301 catcgcaccg ggacgttata tcgtggcgct tgccatcctt gaccgccagg gtggcatgct
    12361 tcccagtgct cgattcgccg tggaaaacta ttttcggggt ggttggcatt cgtttggtta
    12421 tattggcgtt ggtcaatctc cgggtgaacc cactcttgag ggtatttctt ttgacagccc
    12481 cgcattcgac gacagccttc attatgtggt ccccgaggaa ctccgtcgtg ttcgcacgcc
    12541 accgataccc gctataaaac cggtcacgcc gtggacgcct gatccaagtc gcgagctgat
    12601 caatccctgg cggtattgga cgctggattc gggcgacacc tttttggaca aggaggtaca
    12661 tcacgacggc ccggtagaag ggccagccgg acgacgcgtt atccaagtga aaggggcttt
    12721 cggaccatgg gctaccctta actatgaatt cccgcctaac acgccactga cggaaggaag
    12781 ctaccgtctg tccttcctcg tccgaggaac tccaaaggaa tctattgaga ttgagctagt
    12841 ggaaaacgga cgaactgtgg caaaagtggg ccaggttcag cttgaagcac aatggcgctc
    12901 acacacgatc gaattcaaac tggcgaaaaa gctggatgaa aaagggtcct tacgatttcg
    12961 cttaccacgg aacgtcgaag gctccatcga gctgactgac acccacttga aacgcctaga
    13021 ataagagcta agtgtctatt atccgtctga atgaccggga aggagttgat cgccgagtca
    13081 tttattgcga accgagacgc tgattcagga ataccctcgc aaggattcga gggaagtcct
    13141 ccaatttacc ggctctatga atatggtcgt tttcaagcgt tcagaacgag aaaaccgatt
    13201 ggcactgtgg ggaggagcgt aaagatgtct ccaaaaactt gtctagccac cgtgatactc
    13261 gtggcaatca ctttggcgct ttcttgcggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nattcgcatc ggcacgctga acgaattggc cgagggcgtg cgtcggccct
    13441 tcattgaacg tcggggtatt aaattcaaca ttccgcttga tgtgcggacg cccagttatt
    13501 ccgacaatgg tgacagtttt caagcgaata tcccggaggt ttggagcacc gcattttggc
    13561 atgaattcct cgacgagatg gcgcgccatc gctacaacgt gctcactctt tggaaccttc
    13621 acccatttcc atcccttgtg cgggtaccgg agtttcccga gattgccttg gacgatgtat
    13681 ggcgaacccg cttgcgattg gacgatacgt tttcgatgag cgggcgggat atggtgcgtc
    13741 ccgagatgct tcaggacgtg gaaatcgtcc gccgcatgac gatcgacgaa aaaatcgctt
    13801 tttggcgtca tgttatgcag tacgcccagg accggggtat cgaggtgtat tggttcacct
    13861 ggaatatctt tgtttggggt acagatggga agtacggaat cacagcggac cgcaataatc
    13921 cggtcaccat tcgatacttt cgcgcaagtg tcagggaact ggtgcggacc tatccccttc
    13981 tggccggtat agggatcacg gcaggagaaa atatgggccg cgatggcgaa ggcttgacca
    14041 aagaacaatg gctgtggcaa acatacggcg aaggaatccg cgatgccctg gcagaacagc
    14101 cgggcaggca gttccggctc atccatcggt tccatatgac caagttgggt gagattcttc
    14161 aggagtttcg gaactatccg gggccctttg attttagttt caagtattcg atcgcccaca
    14221 tgtattccat cccgaatcca ccctttatca aatccgcact ggaaggaatg cccccgggga
    14281 tgaagacctg gctgacggtc cgcaatgatg acatctacag cttccgctgg ggggaccctg
    14341 attatgcgcg agcatatatc ttgaacatgc cggggccgga tcgactggcg ggcttttaca
    14401 tgggtcccga tggctattgc tggggccgag aagccattga cgtcgatggt gagcttccgc
    14461 gacaactcgt gatgaagaag cagtggtaca gctttttgct ttgggggcga ctgagctacg
    14521 atccccagct ttctaacgac catttccgta agattttggc tgccagattt ccggaggtgc
    14581 cttccgatca gttgtttgcc gcttgggtcg aagcgtcgca ggtgttcccc tatatcacgc
    14641 gattcttctg gggggatatt gacctgcggt ggtttcccga ggcctgcaca cggcaccccg
    14701 ccccaaaggg cttttacacg gtcaaggact ttatccttgg ggagaccatg ccggggagcg
    14761 gtgtgctgag tattcgcgag tggctctctc agggcgaaaa ggctggagag accgcagggg
    14821 tcacaccctt gcagattgcc gaaactctgg aaacgcattc acgagaagcg ttgagattgt
    14881 taaaggaatt gaaaccaaag gaaaagcccc ggcgcgaact tcaacggaca ttgggcgatt
    14941 tggaggccat ggcgtatctg ggccagtact attccgcaaa gattagggga gcggtggcct
    15001 tggcggagtt cgaccgtgat ggcaaacccg aacagcaaga agccgctgta aagcatctcg
    15061 aaatagccct ggcgcactgg aagcactatg cggatattgc ccacagtcag tacaaacccc
    15121 aattgctcaa ccggatcgga tttgtaaatc ttcacgagat tgcggggtat gttgaaaagg
    15181 acatcgccat tgccaaacaa tggcagcctg ggacgcttaa ggacatccct cccggacccc
    15241 gtacagccga tgttcctttc cgcccctagg tgacccagta gagccgatcc aggcagattt
    15301 tttggcggac cctgtccgct ttctcttaag gttcccgagg cgaaacgtgc cggggcgtgc
    15361 tacgccctcg ggcgttttct ttttgccaca cagcgttgtt gggttgttgc tcaggctccg
    15421 cacacgtcga acttgtttga ttggcatcag ctcgcacaag ttcataatta aacgacactt
    15481 acgcctattc tgatgaggcc cagatgtcgc ggcgcggtga ttgcgacact ccgtcacgcg
    15541 ggggagtttt ttcgaaggat cgcgtccgtc ggttcggctg ctgacccgtt gatcaaggcc
    15601 gcgtgcgacc atggcttttc gtatccgccc aagtttcctc tgggtcattc tgcttgcact
    15661 gactgccctc ggggctttcg ccgcggtcgt accaacatcc tctccaaggg cgacgggcta
    15721 caatgagcca gactctcttt ggcgtaagac gaaggacggc tgggaaatcg cctggtggct
    15781 tcagccgccg aaaactttcc acaaaccggt gatccatccc ctcgccgtgg cggcatgtca
    15841 attactggtc gtgtcgttac tgggactgat caaccaccat cagaccagcc gcgatcggca
    15901 atcctcgcgc gtcctccaag gtcaatccgc tggagtgagg agaatagaca aaaccgcagc
    15961 acccacgact ttcccgtcgt ttgtgtcccg tggtgatgcg cccaagcgag actctgagag
    16021 cctttcacac caacggggtt tggatcgcat ttaacgccgc ggatctcttc ttcgcgagct
    16081 ccgccacgtg gaccgctttg tgagggggca agggtatggt ggacatcctt ccgcggccgc
    16141 gcggcactcc tcgcccgtac tactggcact cgccggcgtt ggagtcgtga tcagccctag
    16201 gcctcctcaa ccactgggcc gaaatgggct ccccaagcgg cggccaattc gcgtccttct
    16261 tccaatagtt ggtccatctc ggcctcgctc agcggctcaa agcgttggaa atagaccaaa
    16321 tcttgtttca gttgttctac gctattgatc cccaaattaa cgcttgtcac ccctggcagc
    16381 gaaagggcgt accggatcgc tgtttcaacg cggtccggcc tggccgacgc aagtcgtccc
    16441 acggactgtg ggttaccgta gccgcccttt ttggggtccg caccgccgag gaccttcata
    16501 gcgatgatcc ccacgttatg tttgcgggct atagggagaa ccttctcctc gaagttgtag
    16561 gtgtggcgat caacataact gagaacgacc agcagaacat ccacctctcc cgtctcgaga
    16621 aactgcgcaa atcggcccgg cagattatgc ccggagatcc caataaaccg acatttccca
    16681 gcctgcttct gttttaccaa ccacgtgaat gccccgtccg gatcgagggc cccttccacc
    16741 ttgcgattac caacactgtg atagtaaagc aaatctacgt agtcggtttg caggaggcga
    16801 agggattttt ccagggagcg ctgaccttcc tccacagagt cggccgggac tttcgtggcc
    16861 agaaaaactt ccttgcgacg cgtcttcata acctttccga caccttcttc cgcgcgaacg
    16921 tacgcgggcg cagtgtcgat cgaagtcacg ccaaggtcaa tggccgcgtt gacgacctcg
    16981 gcaatttcgt ccacgggtcg ggcatttcca cacggggcgg tgccaagggt gaagatggtg
    17041 atggactctc cggtacgtcc tagcgttcgc cgaggaagtg gtcccacgga aggagattcc
    17101 acagaccggg cctgctccga cgatactaca ctgcctaata cggcggcacc ggcgaacgca
    17161 ctcgcttggc aaacgaaatc acgacgcgaa agacgatcag catgatcagc gggcatggct
    17221 aactcctgat actttgtgtt gtcaatgcaa gtaacgacct tccctcctaa tcgccacaca
    17281 accgtaaaat atccggggct tggggaccct cgtaccatta cggctatgtg gccaggcatt
    17341 taagtcaccc ataattgttt cgttttctaa cccaaagtca aaagagtgac aacagggagc
    17401 cggagcgagt gatcgtctta catattctgc ctcttcaatg atggccagca ggcgcgctca
    17461 tcgcgtaatt cagtttgcat gagttgccaa tgaattgcat cggctcttaa ttgatggcat
    17521 aatcggggcg ggttccctgg tgttttgggc tgacagtccg ttcgcgatgc gtgtcactga
    17581 gaaggaggtg aggtgtgact aagcagaatc agatctacta ctgtgagcag tgcggcaaca
    17641 tggtggaggt cctggaggcc ggacaggggc agcttgtttg ttgcgaagtg cccatgaaac
    17701 gcatagcgga aaacgtgggc gacgccgctc aggaaaaaca tgtgcctgcg gtgacacggg
    17761 agggcaaatc acttcatatc aaagtgggaa gcgttatcca tccaatgacg cctgagcatc
    17821 tcatcgaatg ggtcgagatg tttcaagagg atcgctgcat ccgcgtgttc ttgaagccgg
    17881 ggcaagcacc ggaattgcgg gtacccgctg cccaggcacc cgctaccgtc cgcgcgtact
    17941 gtaatttaca cggcttatgg aaggttgatg tctgagcggg gaaatatgtc atccccagac
    18001 aacgactgtg ccggcaaata cggcaagggc aagaaacgca caaacgagga gagtttgaat
    18061 cgctatcacc gtcaggtcct ctagcacctt tccaatcggg agaaaaattg gccccgagtc
    18121 tttcatcggg atttgtttag cgaaccacga ggagaaaggt agttcgccag ataaagtctg
    18181 tgatccgcaa atctttcccg atcagaattt tttgggccat gagaagacaa atggttccgc
    18241 agtttcctta gtcccagagc gcagcggcgc ccaaggcggg actgatcgct tcatctcgag
    18301 cgatattggc aaaggcaagc atatctgtgg ccagttcttg gatgcgactg gcggccccca
    18361 caacgtgtgc cacgacgtca ggggcattct tgtcgagggt ttgccgcaat tgctgtgcct
    18421 ccagggagat cgcggcgagg gcctctcgga gggctgctag ccttgtgtct ccagaattgt
    18481 agggaactcc tgcatcttcc tttgcatagg ccttggaaag aacgagattc ctgaaccagc
    18541 gcaggagcga accccgactg atggcaccga tggggacacc attgtccgta atgatcaccc
    18601 gtcgtatcga aactcggcat agaaactcat aaataatccg tacgggagcg tccagtggat
    18661 aggtgatgac gtggggtcgc atgagatcgg aaatcggacg actccaccca tgtacagatg
    18721 tcatggccga cagaaggtcc ttttctgaca tcataccaat cagtcggcct gtttcatcca
    18781 cgacaggagc cgaactaatg cgcgatctga gaaagaactc agcagcctga tccaccgttt
    18841 cgtcgccacg taatggggca acaatcggcg acatcacatg gcgtgccagt atcccttcga
    18901 acaaatcacc ttgccggtgt ttatcgctca ggtttacctc ttcacttaag ctgatctcat
    18961 gataacgaac gagccgatcg cgaccggcac gtttggcaca aagaagggcc tgatctgcct
    19021 gatcgacgag ctgttctggg gttagcgtgt catcataaac ttgagagatt ccgatactgc
    19081 atgtcagaga aagcgttgtt ttcccgatcg taaatggtgt ggcgcctagc tccgcgcgaa
    19141 aacgttcggc ccacttgacg gcgttttctt cagttgcgtc tggcaaaagc acgcagaact
    19201 cttcgccccc ataacggcac acgatgtcgc tggcccggca acttgccaga agcaactggg
    19261 ccgttgtcct taagacgtaa tcccctgcct ggtgaccgtg gatgtcgtta atacgtttaa
    19321 aaaagtccaa atcaaccatt gcgcagctaa ggggccgctt tgtcctccgt gcaacttcaa
    19381 actctttttc gagaaaatca tgaaagctcc gctgcgtcag caaacctgtc agggcatctc
    19441 tttgggccat ctcttggagc tgtcgctggg cggagaggat acggatggcc gcccccagtc
    19501 gggccattaa ctcggcttcc cgaataggtt tggcaaggaa gtcatcggcc ccttggctaa
    19561 gcgccgtgat tagattggcc gactcgttat gggaggtcac gattaggaca taaacataat
    19621 ggggaagcct caggagcctc atttcacggc acagatcagg ccccttcatc cccggcattt
    19681 cccagtcagt gatgagaatg tgaggcggtt cctcgcgaac catttgcaag gccgtttccg
    19741 catccttggc ctggctgact tgataaccgg ccttcgtcaa ccaacgttcg agtaaccgca
    19801 agattagggc atcgtcatcg acgattaaga cgcgaaatgt ggcaggcgaa attccttgca
    19861 acgacgaaga aaaatcctcc ggcggcatcc aggtatgcct gtgggccgat tcctggagct
    19921 gtgacgaagc aaaggaattc gcctggtatt ctcgtggttt gtcttcaaac ctatccatgt
    19981 tgcgcactgt cattgctcct taggttcgcg acgtcaaaaa aacatagact accgtttcaa
    20041 ggaaggcgtg ataacaccgg cagttttttc cggttaaata gatactttca atttctcagg
    20101 tgtggtgggt ggcggatttt gcggaacttt ttggaatagc ggttatgccg ctttttcttc
    20161 gggcgaagtg gagaaggact gtaccgaacc tgctgagaga atttccccct gatcgctcaa
    20221 atcctccagc cgtacggaga cccgttttga tacacccgac tcttgcatcg tgaccccgta
    20281 cagcgtatct gcgcagattg ccgtgcgttt ggaatgcgta acgataacaa attgcgtgat
    20341 gctgagaaaa tcccgcagaa cctttgtaaa acgatcaatg ttagcctcat caagggcggc
    20401 atcgacttcg tccaaaacgc agaagggact cggacgactt cgaaagatcg caagaagcag
    20461 ggctacgcat gtgagactct tttctccgcc actaagcaag gagatgtttc gtggttcctt
    20521 ccctggcggg cgggctacga tttcgatgcc actttccagg atgtcccgcc ctggctcaag
    20581 ctcgatgctt gcctgaccac cgccgaaaag ttctcggaag agggtgccaa agtgttcact
    20641 gaccgtctgc aatgtcgcgg caaaaagccg ccgactatcg gcattgatcc gctcgataat
    20701 tcgttccaag gagttttttg ccgaggtcaa gtcccgacac tgggacgaca agccgttgta
    20761 gcgttcttcc agttgttgaa gttcctcgag ggcttcgagg ttgacgttcc ccaaattgtg
    20821 gattttacgt ttcagatcgt cgatttcccg ctggatttca gtgcggtcct gcgactcctc
    20881 aggggaacac ggatggtcga gatcgttcag agaaatccca taatcttctt cgactcgctg
    20941 gatgagtgtt gacttttcgt gccgcacaga gttgagatca agttccagtt tgtgctgcct
    21001 ctcctcgatt tgacggagcc gtcgccgcac atcctgcaat tgttgctgat gtttttgacg
    21061 ttctttctgc agggaacgac gggcctccgc ggtctcttgc caaacccgcg aaagttcatc
    21121 cttccgcccg tacaattcgg ccaggcgggc ctccagactc agtagttccc gttgtgaggc
    21181 catcaaccgc ttttcggttt cagccaaacg gtttttgatt tccgtgacct ccttaagccg
    21241 ttcggcctcg gcttcctctt gccgtcgcag ttgattctgg agggccgcga gccgttcttc
    21301 gctcttggcc aattcgattt tcaacgcggt caacacaccg tgacgatctt gaacattcgc
    21361 ttccaattgg gcaatttctt ctcccaattg agtgatggcg atggacaact ccgctgcctt
    21421 ttcctcccat tgcgtcttct ctaaaagtgc ctgctgctgc tgttggacaa ttctttcaac
    21481 cccctcggca gccgcggcaa tttcgccctc cagtgctcca cagcgtccca aatactcctg
    21541 caacatccgt tgcagctctt gttgccgttg ctcacagtct ttcagttcct gactgaggga
    21601 acgacgctgt tcctcaagat tggcaattcg ttgctccaca gtcttgagct gatgttcact
    21661 tgtggcaatg gcttcctggt actgttgcaa ctgttggtcc cattgggcaa tcgaacgcgt
    21721 cagattacga agttcgctcc ggcgcgaaac cagcccaagt ccagaactct gttgcccagc
    21781 tacgataagc ccctcggaag tgattaactc cccggcaagg gtcacaaatc tcaagtcggc
    21841 ataccggctg gccaaagcta gggccgtgtc caaggactcg acaaaccaag tcttgccaag
    21901 cagaaaatgg cgaaccggca ggaattcttc ttccacctga acgaagtctt ccgccagccc
    21961 caggactccc ggttcctgcc ccagggaaga aatgtcagtg ggcatgctct ggccatcggc
    22021 cttttttcga aattgatcaa gacacaacag acaaacccgg ccgttgagtg ccgcagaacc
    22081 gcggcggagt tggtccagcc aaggcatgct gttgcgcaag acgatccatt ccgcggcatc
    22141 acccagggcc acatccacca aaagggctac ctgaagttct gtctgcaaca gatcggccac
    22201 caagccacaa acggaatcgc cgagatggct ctccgatgtc tggacgcgat gaagaaaatc
    22261 cttgacaccg aggcttaagc cctcaagttg ttcctcccac tcccgcagca gccgtgccct
    22321 ttctgcggca gccgttcgtc tttggtgcag ggccgaccat tcttccctaa tctgttgtaa
    22381 ctcctgacgg caacgatcgc gttcttcaga caaccgatgc aaatcctgca atgttgtctg
    22441 aagcctttgg aggaggtcgg cggagtggtt cttcagttca tcgagccgtt gctctgtttc
    22501 gccaacaatc cgtttaatct cttccgattc gcggctattc cgttcgtaac tcgcttgcaa
    22561 attctcatgt tctcgctgga gctgctgtaa acgattgtca gccgcgagga ccttttgcaa
    22621 gacttcccaa tgctcggctc gccgctcctc gtattcagtt cgacgacggt caagcgcttc
    22681 caaatcgttc gttaaggacc gttcaccctc cgcaacttgc cgcatcaaat cgcggtactg
    22741 ttcttctgcc gcgcgaaggg cctcggccgt tgattggcgg tgcgagcaga gttcccctgc
    22801 ccgcaggtta agctcggcca acacctttcg cagccgacct agttcctttt ccaactcacg
    22861 gtcacgtgcc cggccgtgct caagatcggc ctccaaggcc gcgatggtct cacgatctgc
    22921 agccaattgc gattgcagat cggttaactg ttgggcaaga tgcgagtcct gagattcgat
    22981 ctccttgaga cgcctttcca gttgttcggc ctcctcgaca agcgtctctt ggtctctgtt
    23041 aagttgtgat ccttcttcct ccaggcgggc cagctcccga ttgagccggt gccagtccat
    23101 tcgggcgagg tgagtccgca attccttcag acggtcggca taacgttgat atcgttgagc
    23161 cttgccagcc tgcgatcgaa caacacgcag gcgaccctcg acttcttcga cgatatcatt
    23221 gagtcgaagg aggttctgtt ccaggcgttc caaccgccgc tgcgcttcga gcttgcggct
    23281 cttgaagcgg ctaatcccgg cggcctcctc aaagacaacg cgacgctcca gcggcgtggc
    23341 ctgcagaagc tgatcgatct ttccctgctc gattaccgaa taggcttgcg tggccaaacc
    23401 tgttcccgca agcaagtccc gaatatcgcg cagacgacag gtctggcgat tgatgaggta
    23461 ctcactttcg ccgcttcgat acaaacggcg cgtgatatga acctccgggc tgtcgagcgg
    23521 taaaatgccc cgagaattgt ccagcgtgat ggtgatctcc gcgaagttca tgggacggcg
    23581 ggtcgccgaa ccgttgaaaa ttacgtcggt catttccttg ccgcggagat tcttgatgct
    23641 ctgctcgccc agcacccact tgatggcatc gacgacgttc gatttcccag acccgttagg
    23701 gcccacaatg gccgtgatcc cgggcctgaa ctcgaaccga gtccggtcgg caaagctctt
    23761 aaacccgtaa atctcaaggg cttttagcat gacgtgaaca acaaaaccag cttcaccggg
    23821 aaggacgctc ttcaacggca ccgagccaca atgtccaaag atgatccagc tagtaaggat
    23881 ttctttagtt acccggcgtc cacaggacgg cggtaagata gcccaaatgg ggtatcgagt
    23941 gacaaaacgt tatcccctca aggatacaga atccactaag tcgtgacgag atcggtttga
    24001 cgcgtcggac acctcttaaa tgtgtacgag catacatcta tagcggggta atgacgcgct
    24061 ctttaggtgt cttcgagggg ccccggcgtc tcaaccatca tgccgggcgg tagatggctg
    24121 aacatctggg tgtttcccac agttgggtct ccacgacggg caagcccgca gcccgcacgt
    24181 agtcaaagat atgccgggcg atattctctg cggtggggtt ttcggccatc aaatagagtg
    24241 gctcgcccat ggactgtagc acaggcacga ccgggtcctc tcggtgaagg atcatccgat
    24301 gatccagatt ctcgtcgatc cacccttgga gaaccctctt aatatccgcg aagtcgagaa
    24361 ccatccccag tttgtcaaga gaatccgtct caaaagttac aagaagtcgc ccattatgac
    24421 cgtgcaggta acggcacttg ccctcgtatt ggagcaaccg atgaccataa caaaaatcaa
    24481 tctcgcgtgt tacgcggtac atacttttcc ctttggcgtg agtgcctccc tgatttgcgc
    24541 gtgtatgacg tttatggtgc ctaagcccag agaaaactct taactagcta tgcccatgtt
    24601 caatcttgct aaatgcatac agtgtccgat ccggtatccc ggcctcctga aatgcacgct
    24661 gccgctccgc gcatttattg caacgcccac aatgtcggcc gtcaatcggc gcaatgcatg
    24721 agaacgtctc ctcaagtggt aatgtccgct cccagtgcat gatggcggtc ttgctcatgc
    24781 cggccaacgg ccgaatgatc cgcacttcag gcggaccata aacgtttagg agatcttcca
    24841 gcttcgtgaa aaagccttcc gctgcatcag cgaagggatt gctatcaagt accccgatgg
    24901 ccaaacgagt cacaccgtgc tgccggcacc agagaccggc cacggtgagg agtaggagat
    24961 tccgtccggg caagaagacc gcttcatcag gcgaatcggc gctgggaacc ttttgnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcat ctcccacttg cggcattttt
    25141 ctcttgctta catctgtggg gggcatttaa aatccatata ggatgagatg gccacccggc
    25201 ggggtggaca cttttggtac atgaatcggc gggccaggta aaaacacatc gggagaacaa
    25261 gcgtggtcct ctccgagatt gatcgccatc tcctggaacg ctgtctcgaa cgcaagccgc
    25321 gggcatggga agactttgtc gatcgttttc tgggcctcgt aatccacgtc atcaatcata
    25381 cggcccgcag ccgaagtctg cgaatgacgc ccgagctccg cgacgacctc tgctctgaag
    25441 tctttttgac cctcctgaag gacgattttg ccgttcttcg acgttttcga gggcagtgca
    25501 gccttgccag ttatctttgc gtcgttgtcc gaagggttgt cgtcaaggcc cttttgcagc
    25561 ggatgggtga ttcacacctt caagaatcct ccagcgttcc accgccaaaa ggattgacga
    25621 catcatcgga agaagaacgc atcaataacc gtgaggaagc cgaaagacta ctcagcctcc
    25681 tcgagggaaa tgaggccacc gtggtccgca tgttccacct ggaaggccga agctatcagg
    25741 aaatcagtga ggctacaggc attccgacca acagcatcgg ccccattttg agccgggccc
    25801 gcagcaaact ccgccgaatc accacggcgt agcctcatca agaataccga tagtcaaagc
    25861 ggccgttgta cgatctctcc ttgcgatttc ttactcttct tatggctgga actgaagggc
    25921 agtgcgagcg taatgctggg gatttagtcg gaaacgggca agggacgcag tgcttgagaa
    25981 catgtatagt cgcccatcgt aaaccgcgca gtgctcgatt ttgccttccg ataggacacc
    26041 ttgatccacg agtaggacag ggtctcggcc attgcatacg gggacgtatc gttccgggtt
    26101 cgtttggaat ttccgttttt gtgtggcccc accacaaaag tacgtcttgc cttgatattc
    26161 caccgcaaac cgagcctgcc ccttaaccca tgcctcgttt tccacgagct ccaccgggca
    26221 ataaccgttt aacgcgggga ccacatcatt ggccgtgact gaagcgaccg gtgctgtcgg
    26281 agaatttgcc actgtcgcat ccttcgcgct tgggaccgtg aagcctgcag gtgtgaccct
    26341 actttctgcc gagtcctcag cccaaacggt gacggccgga cctggttcag gcgtcgcaaa
    26401 ggtggcggta cttgggtttc cttgggcaac gtgcgtggaa atgctgggtg tttttccagg
    26461 cagtggcgtg tagttgcttg ttgccacact gggaagccac ggcgctacgg cgatgtccgg
    26521 taccgactcg ctacgagaga tttccaattt gttttgcggc aaaggacggt ccatcggagt
    26581 agttggggta cttgcaacca cggtggggtt aacgatggtc aacggccctg gttttgacga
    26641 ctgggcaaca ccgctcgtcc cggttgtctt tcccgaaggc cgcgacgcca aatcatttgt
    26701 tccggcaata acccaaggtg gtgtgggcgg cattggggaa atttgtggag cactgtaggt
    26761 tgaggaactc acagattggc cggagtacgg catgcctgaa agctgcgggc ccgaaataat
    26821 cacccctgac gttcccggag agccggcagg catcggattt ggggccgccc cagccggtgg
    26881 cataggttgc gccggaggag gcgtgctcgg catcaaaccg ggcaggttgc ccgtttctgg
    26941 ttgaggttgc ggcgaggggc tcagtggcaa cgaagtcgcc ggtggcacac ttgtgggagg
    27001 catttgggga ggtgcttccg ccggtggcac aggtggtggg gacggtggtg tggccgtatt
    27061 aagggaagga actgccggcg gcatgggcgc agatggcggc ggataagcct ctccttgtgg
    27121 ttccggcatt gtgtcggcgg gagacggttc aggaaacggc cgtcgcggtg tggggatgat
    27181 tggctgcaga gtctctttgg gcaattcagg gcgagactct cctgccgggc gcttcgtcgg
    27241 ctccgcgcca atggattgag ggaaatgaat atccggacgc gactctccgg gccaaggtcg
    27301 ccaacgtgtc ggataatgcc caaagaactg aacatttgcc tggcaggtgg cgcacgatgg
    27361 acagtagtag tctgccccgg acacaacact tacactaagc caggttaccc cgacaagcaa
    27421 tcccaccaat gaacgccaaa cggtcatcct ttttgtctcc taaatacaga caccgtatgt
    27481 tgtctcacaa agggttatgc caagtcatta ccagccgcta cgggtgcggt tatacccacg
    27541 gcccatggtc tggagggaac ggactgtgga ggttcccaga ccggtgaacc caaacagacg
    27601 ctccaccggg gccactgtct tgtcgatcca ggcatgaaag gcaatcatgt cgtcatgggc
    27661 aatgaagatc cggtccccag gcagtacctg gtaattggtc gccgtattgc caccccgcgt
    27721 aatggcgttc caatccacgg gcaaaatctg ttcgcagcct gttccagccg gggaaggccg
    27781 ggcaatccaa atccgtttgc tggagacctg ggaaatccca cccacttggg caa
//
LOCUS       DAOSUT010000038        27759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1128_length_27759_cov_8.880811, whole genome shotgun sequence.
ACCESSION   DAOSUT010000038 DAOSUT010000000
VERSION     DAOSUT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27759
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_27759_cov_8.880811"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="PLD05_05150"
     CDS             complement(<1..1581)
                     /locus_tag="PLD05_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOP76855.1"
                     /translation="MTKASVWQRIALRAAEGTPVRKVVASGAGRPWFFCGVFATRRGW
                     RKDRRGQRGMILLVVLVTVAILSLAALTLSDLMLAEREGVKVAVQIAQARAAAMSGVE
                     AARFFVSMDEEWQNDNGGWFNNPSWFAGVVVQEGADPRETSLFSIIGPDWSDYGASAI
                     RYGLENESAKLNINSLLLADKTVENGGRQLLMALPGMTEDIADAILDWLDADDEPREF
                     GAELEYYTTLPTPYTPKNGPLETIEELLLVRGVTPELLFGADRNRNGVIDPGETIPEV
                     FANLSADDPVAYRGWSAYLTLFSMELNVRPDGSPKIDINQSDLEKLYDEIEAEFGPEL
                     ATFIVAYRQNGPYTGEEQGEPVPLNASLDFSRQAKVKFDTVLDLIGAKVRVQFAGEQE
                     PRVLDAVVPEDPVALRVILPVIMANLTATSSKVIPGRININLAPPSILYGIPNLDPGV
                     ADLILEARPSDPTFIDDENYYYETWLLTEGLVTLEEMKALMPFVTCGGSVFKAQVVGY
                     FAGGGPSCRVEAIFDATVR"
     gene            complement(1578..3404)
                     /locus_tag="PLD05_05155"
     CDS             complement(1578..3404)
                     /locus_tag="PLD05_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76856.1"
                     /translation="MNLTKRQTISFGSKAGMSIAPWGAGQSGGFLALARKLRRTRGLT
                     LLEVLLSLGLIAILLAALGTAVDFHYRVVQSARHQVEETQLARVLLDRIAQDIRGAVS
                     YDPVNFEELIPGLVTKSGSSSNLSSLAEEAGIDPSLLLESGNEMGTYSTGGDWTSTTL
                     PRSVPGLYGGSNWIEIDVSRLPRPDQWQSEYALAADGLLVDRISDVKTVAYFVLSPEE
                     TINYSFVSMPGVVGFDQQGGLVRRELDRAITAYAAMTGELEAVDQQLQPIASEVAAIS
                     FAYFDGSAWCDSWDSDAQGSLPKAVEIRLYIRSSQGSQSQNGGILSGLSPVADVTTDS
                     GSLSLGLLDDLSNCRVYRTVVYLPAAHETGLLGATSGSESEEETVSEGEEEMAPEEEP
                     PPEQMPEESDEEGPGMGPGPGGDFGGGPGQGGQGERDRGGDRSGGWGGRGERGGGDFG
                     RPGPRQGEGPSSGGFGPGGGPGGGFGGNRGGGGFGGPGGGDFGPGGGFGGGRSGGGMG
                     PPGGFVRGGGGPPGGGFGGGSGGPTGGRPSGGFTPGGGFGGSGAGGGRSGGGMGPPSG
                     FVPGGGGPSGGGFGGGPMGGGPSGGFTPGGSSGGAFGGGPRQ"
     gene            complement(3657..4199)
                     /locus_tag="PLD05_05160"
     CDS             complement(3657..4199)
                     /locus_tag="PLD05_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP76857.1"
                     /translation="MFTCEKKLPTSNKPDRHRRDGFTLLEVLLALAILLTSLAAIGQL
                     VFNAYQNALRVKNLTRAQLICEGILAELNAGSLPLETIADAPVEDIDDTVEPGWLYSI
                     DIGELDTNGLLSVRVTVYQDPSLFARPVELTLTQWMIDPTVEFVQPGEKTGSEGMESS
                     ATSESASTEESTGQEGTGSR"
     gene            complement(4261..4983)
                     /locus_tag="PLD05_05165"
     CDS             complement(4261..4983)
                     /locus_tag="PLD05_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76858.1"
                     /translation="MSLVFGRTYAARKVQWTVVPPGRSKRACGDVPRLAFTLLEVVIV
                     LVLVVILSGVAFNALMRPMAARRLQLAADQIRAEWIRARNRAMANGQTYYFQCELGGN
                     GYVLVPYDASVPGTDVYDPSMGTQDMTDVPLMQIPGGGSRKELPEKITFVDVQVEGGE
                     LTAAITEDPTLSSPPSDGSSILFFPDGTTSSALITLENEFGRRIDISLRGLTGAVLVG
                     ELYDSESAGMTELPAAPSVAGS"
     gene            complement(5032..5484)
                     /gene="gspG"
                     /locus_tag="PLD05_05170"
     CDS             complement(5032..5484)
                     /gene="gspG"
                     /locus_tag="PLD05_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOP76859.1"
                     /translation="MRQTKGFVRKKLSRTTRKGLTLIEVLIVLTILLIIASLAVTNLI
                     AVQRRAYINAARTQIKAFEQPLHAYYLDFNEYPPSLDALITPPADLADPTKWNGPYLD
                     AQAVPLDPWGREYQYLYPGQYNTDKPDIWSMGPDGVDGTEDDITNWGQ"
     gene            complement(5960..7171)
                     /locus_tag="PLD05_05175"
     CDS             complement(5960..7171)
                     /locus_tag="PLD05_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP76860.1"
                     /translation="MPEFSYVAVDANGQRVTGTIAAATKREALTAIASRELYPLEVRS
                     EAPARPLFSFGRRSVPAQAMAVVYGQLADLLRSGVPLLRALNIVKSQSVHPTLKEVLG
                     EVARRVEDGMGLGDAMARFPGVFSEMASSMVRAGSEGGFLEEALYRVAEFTEATEDLR
                     KRVKGAMAYPMFLIVIGSLLVSGLLIFLVPQFEGMFQNLRERNELPGLTEGLLAFSQF
                     LQAWGIWVVVVIILGFFSVRAWLRSETGRRLWDRYVLKVPILGGIVLNFAVARFCRVL
                     GTLLKNGVPILRALEIASEATGNVLLGEAVHKATKNISQGQRLAEPLAACKLFPPLVV
                     EMIAVAEEANTLETVLLQIADSLERRTWRQLDVGVRLLEPILLLLVAMAVLVVVVALL
                     LPVFKMSGAVG"
     gene            complement(7246..8946)
                     /locus_tag="PLD05_05180"
     CDS             complement(7246..8946)
                     /locus_tag="PLD05_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOP76861.1"
                     /translation="MEAGEILVRKGLLTPQQLQLARRHQTEGKRLDQAAVGMGLVSEE
                     DALRALGEELGIEYIDLSEATIDPSILKNFPVKFIYRESLFPIREENGRLLVATSDPF
                     NLYPLDELATATGKTVVPVLASQAEIAKLIKTYLGVGGETIEELMAQGEEEESAVELL
                     DEIETDGSELSKLAQEPSVVRLVNEILLEAIDLRASDVHLESQPSGLKIRYRIDGVLH
                     PQPVPPEINRFHAAIISRLKIMARLNIAERRLPQDGRIQLRVAGREVDVRVSVIPMIH
                     GEGIVLRLLDKGAMEFDLRKLGMEQDVYEKFRELIQLPHGILLVTGPTGSGKTTTLYS
                     ALLEIRDESIKIITTEDPVEYQLEGINQIQVHPKIGLTFAHSLRSILRHDPDVILVGE
                     IRDLETAENAIQASLTGHLVFSTLHTNDAAGAYTRLVDMGVEPFLVSSTVEAVMAQRL
                     VRTLCPECKEPYRPRPEELPKDFPREILEQGVTLYRAVGCRACRHVGYRGRVGIFELL
                     VTSERIRELAHDRASSWVIRQTAIEEGMITLRQDAWRKVLSGRTTIEEIERVTRAEEH
                     "
     gene            complement(9491..10258)
                     /locus_tag="PLD05_05185"
     CDS             complement(9491..10258)
                     /locus_tag="PLD05_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76862.1"
                     /translation="MRKYVLSLVTVVGMAFALVAGTAMAQPPGGPGGPGGRGPGMFMM
                     GPGGGVGYAQLLRIEKVQKEIEMTDDQKAEVEKILEKSRERMRELFQGGGQDREAARQ
                     RFQQAQQETQKAIEGVLLPDQVKRLKEIRLQVIGIGAAMMDQEVRKELGVTEEQAQKI
                     RDAVQKVMEELRGQRQQGERPSPEQMRERMQQMQQKMEEAVMSQLTEEQKNKWKSMIG
                     EKFELDRSELFGGFRGGPGGAPGGAPGGQRRGGNRGA"
     gene            complement(10416..11729)
                     /locus_tag="PLD05_05190"
     CDS             complement(10416..11729)
                     /locus_tag="PLD05_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP76863.1"
                     /translation="MPLWKIAWRSIQHRAVASALTAFSMALGVALVVAVLVIYHVLDR
                     SFRRSSQGYDMIVGPAKGSPLEIVLSTVYYLQAPRGTIPYEIYEELSHGRFATEVAAA
                     IPVCIGGSYAGFPVVGTVPELFEEFRYLGDRPYEFEPGGRNFSPDAPFEAVIGATVAR
                     RAGLRVGDTFRVTHGLAEQGGHEHPDEFTIVGVLKPTGTPNDRALFVNIEGFWTLHQH
                     DARQGKASEEEESVNPMDGDGDEATSETEAHDENHRELTAVLVKRDWTNSAKALALPM
                     QVSKEPGVQSVIPTEEVAKLFEGLLGNIQTILLIFAVLIVVVAGIGLMVSIYNSMNER
                     KLEIAIMRALGAKRSTVMAVILLESILLSLGGGAIGVLLGHGLIGAFSGVILEETGVR
                     VSALSFQTVELILVPGLIILASVVGYLPALVAYRTDVAKAIASGV"
     gene            complement(11817..12515)
                     /locus_tag="PLD05_05195"
     CDS             complement(11817..12515)
                     /locus_tag="PLD05_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76864.1"
                     /translation="MVFPQDESPLLVLRDVRKSFQEPDGGELPILDIPEFTVEAGEQV
                     VIVGESGCGKTTLLHIIAGISRPDEGQVRINGWDITLLSEAECDRFRAENIGYVFQTF
                     NLLPAFTALENVLLGMTFGRGKPDVARAKHLLERVGLAHRMHHKPRQLSVGEQQRVSV
                     ARALANRPKLILADEPTANVDSGNQQAVLDLLREGCREENVALILVTHSPEVARQFPR
                     IVRLPDINLVLTRK"
     gene            complement(12717..14726)
                     /locus_tag="PLD05_05200"
     CDS             complement(12717..14726)
                     /locus_tag="PLD05_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP76865.1"
                     /translation="MNHFLQVVQIALRRPLWIVAIAVTALFVGVMWGGNITAIYPIVE
                     VAFRGESLHYWIDLKIQECDEEIARLQEEIAAAGGTEADGPSADGSDREAGTSHRPVR
                     SGQLSQLLVKRDWYLWLRPWILRLTPNDAFSTLLFILSLLLIGTVLKSVALVINSVLV
                     ARVAHQTVFELRRRFFAHVLRLELSKPGDEGVAHLMSRFTHDINCIYSGLSVLLGKMV
                     REPLKMLACLVGAAWVCFPLLVFSLLVVPPAAWAIWKLGKLLKRANRRAMEEMAQLYG
                     TLEEALRSLRVVKAYTMEPYERRRFHETSRRYLNKGMRIAVFDALTHPLTEILGMSII
                     SLAMAAGAYLVLNEKTHLLGLTVSSRALTMPELVLFYALLVGAADPARRLSDIFASLQ
                     AAMAASDRVFAMLEREPEIKDRPLARRIPRHRRDLVFDHVWFSYTPDQPVLRDINLEI
                     PFGETIVIVGASGSGKSTLLSLIPRFFDPNRGEIRLDGVPLPDIRLRSLRKQIGLVPQ
                     EPVLFNDTILNNIKYGSPQATDEQAIEAARAAHAHTFIEQLPQGYNTLLGPMGCGLSG
                     GQRQRIALARAMLRDPAILILDEATSQIDVESEQLIHQALERFAQGRTTIIVTHRLNT
                     IAMADRILLLSGGQIVGLGKHHELLEQCELYRRLFAGSMWELRAA"
     gene            complement(15097..15669)
                     /locus_tag="PLD05_05205"
     CDS             complement(15097..15669)
                     /locus_tag="PLD05_05205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOP76866.1"
                     /translation="MKVLVVIGHPRPGSFCHAIAETAVKSLQSKNHEVIFHDLYQERF
                     DPLLPADQIPKDATVPPEIEEHCRQLREADGYVIVHPNWWAQPPAILKGWIDRVFRQG
                     VAYDFSAEGVVGHLKGKRALVFTTSNTPREDELRLYGDPLENLWKNCIFGFCGLTHFR
                     RRNFESIVMSTYEQRVGWLEEVRQIVESFF"
     gene            complement(15759..16217)
                     /locus_tag="PLD05_05210"
     CDS             complement(15759..16217)
                     /locus_tag="PLD05_05210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOP76867.1"
                     /translation="MPVLFYYVHTVQPSEIDPLNHANNVVYVQWLQDAAVAHSAARGW
                     PPERYLALGAGWVVRSHTIEYIHPARLGDEIVVQTWVADFKKVTSRRRYRIYRVNDGC
                     LLAQAETLWAFVTYATGQPTRIHPEVVASFKLPEGDPPPYRPGELSLVKS"
     gene            complement(16575..17315)
                     /gene="ispD"
                     /locus_tag="PLD05_05215"
     CDS             complement(16575..17315)
                     /gene="ispD"
                     /locus_tag="PLD05_05215"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOP76868.1"
                     /translation="MPRFAVILPAAGASSRFRDPHYKKPFAPLAGRAVWLHSLHRFTQ
                     RDDVKQLIVAIAPDDREEFLRRFGADIALLGVEVVDGGRERVDTVSNALTRVREEIDF
                     VCIHDAARPCISDLWIDAVFQKAVETGAAILAVPVTATLKKVDDSGKIVETRSRDKLW
                     EAQTPQVFRRDWLLEAYQKRGDFVPTDDAQLVERLGKAVYVVSGSPLNIKITTKDDLR
                     LAEQILKILPKPRASGPLHPFADDDLWR"
     gene            17830..17905
                     /locus_tag="PLD05_05220"
     tRNA            17830..17905
                     /locus_tag="PLD05_05220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17866..17868,aa:Pro,seq:ggg)
     gene            18423..19016
                     /locus_tag="PLD05_05225"
     CDS             18423..19016
                     /locus_tag="PLD05_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOP76869.1"
                     /translation="MLHQQIDWELAEREAWALRRQKGIPNNRRLFVLACMDERLPVED
                     ALGIRVGDAHVFRNAGGLVTDDAIRSAMLTTQFFGTKEIIVVNHTECGMMTATGDYLT
                     NLLREKGIDPDAVQVDPALPELKLPPGTFPKWIRTFTDVDEICLRQVELLRSSPLIPA
                     DVEIHGYVWEVESMRLRRPGVRLAEKVNTAKAMGARG"
     gene            complement(19225..21210)
                     /locus_tag="PLD05_05230"
     CDS             complement(19225..21210)
                     /locus_tag="PLD05_05230"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HOP76870.1"
                     /translation="MASGHPYRDFEEYDALKAADHVPEPVQVFGPGPEGRLPFDDTML
                     RYDPSGHLFGMSQDAGMGWDPAETRRPQFVILSTLGGLRDSEGKPIALGYHTGHFELD
                     EAVAEAAKEIRRLGGMPYAGFCSDPCDGRTQGTPGMFDSLPYRNDAAIVFRRLARSIP
                     RAVGVLGIATCDKGLPAMMMALAGLRDLPVVIVPGGVMLPTTRGEDTAKVQSIGARYA
                     QNEITLEEAQAAACHSCASPGGGCQFLGTAATSQVIAEALGLALPHSALAPSGQPLWR
                     DVARRSAQALVYLAKKGLAGKAILTEAAAHNAMVLHAAFGGSTNLILHLPAVLYETGL
                     PRPSLEDWERVNRQVPRLVDVLPNGPKNHPTLYVYLAGGVPEVMLHLRDLGLLRLDAL
                     TVTGRCLGEILDWWERSPRRHRLRELLRRETGIDPDEVILSPNRAVALGMTGTSCFPR
                     GNLAPGGSVVKSTAIHRRVLDDNGVFDRVLRARVFVREKDAVAAIKGQTSHPVQPGDV
                     VVLIGRGPMGSGMEETYQITSALRDLPWGHEVAVITDARFSGVSTGACIGWVTPEALT
                     GGPIAKVRDGDRIRIVIDTRALQGSINLVGDAEREFSPEEAEHVLKSRTPHPELRPDE
                     HLPEDTRLWAMLQHISGGPWAGCVFDREAILRRLQQT"
     gene            complement(21226..22263)
                     /locus_tag="PLD05_05235"
     CDS             complement(21226..22263)
                     /locus_tag="PLD05_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HOP76871.1"
                     /translation="MWKIHVAQAIRDPAELCSLLDLPPELADEARTAMGNFPLLLPRP
                     MLSRIKPGNPADPVLRQFLPTMPETAAEPGFSTDPLQEQSACPLPGVLAKYEGRILIL
                     ATRACPVHCRYCFRRYFPDTPPTSSPRQTGSRKLPNNPQDVEGSPPTSDGYITSICRY
                     VQSRPDCHEVVLSGGEPLLIDDQQLGTWFSLFSQIAHIQRVRIHTRLPVVIPQRVTPQ
                     LIALFRGAPFRSVVVFHINHPQEIDDAVAEAITRLGHSGAILLCQSVLLKGVNDDPDV
                     LATLYERIAGLRVLPYYLHQLDRVLGAHHFEVPVDRGKKIIEELRKRLPGYLVPRYVR
                     EEPGKDAKVPL"
     gene            22596..23168
                     /gene="efp"
                     /locus_tag="PLD05_05240"
     CDS             22596..23168
                     /gene="efp"
                     /locus_tag="PLD05_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOP76872.1"
                     /translation="MATYNTSEFRKGLKVQLDGEPYLMIECFFVKPGKGQALYKCKLK
                     NLIRNTVIDRTYKSGDTIEAADIEEIDAQFLYRQGDQFVFMDNNTFEQYELTKDQVDE
                     AWKYLKEGMPCTMLLFNGKPISVTPPNHVVLKVEYCEPGARGNTATNVTKPCTVETGA
                     EIIVPAFVEIGDLIKVDTRTGEYIERVKAE"
     gene            23186..24187
                     /gene="epmA"
                     /locus_tag="PLD05_05245"
     CDS             23186..24187
                     /gene="epmA"
                     /locus_tag="PLD05_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOP76873.1"
                     /translation="MSWGRAPDDFRPTANWEVLRLRAELLRVIRQFFDERQFLEVETP
                     ILSADTVVDRHLDPFWTADVNRPADITPQTRLYFLQTSPEFGMKRLLAAGSPKAIYQI
                     SRVFRRGECGPLHNPEFTLVEWYRVGDDYHTGMDLLAELCEVLFGRGRPRIYTYGEVF
                     TRFVGIDPYSAAADQLRQRAEALGVAYPASLSLEDRDGWLDLLFTEKVQPHLGREQPV
                     IICDYPASQAALAIVRKGPPSVAERFELFVNGIELANGYHELRDPEELRQRITTANAQ
                     RTREGKSPLPPTNRLLQAMEAGLPACVGVALGFDRVVMIRAGANSIREVLAFPFDRA"
     gene            complement(24357..26111)
                     /locus_tag="PLD05_05250"
     CDS             complement(24357..26111)
                     /locus_tag="PLD05_05250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOP76874.1"
                     /translation="MCTVRFPLRNAVIGLMMGLPLIAWAGGTVEEPPDWIFHHGRIVT
                     VDKDFRVVEAMAIRGDRIVAVGGNDEVMRLAGDQTRLVDLRSKMVLPGLIDTHSHPVG
                     AAVYEFDHEIPTMETIADVLAYIRQRAAVVPEGKWIVVQQVFVTRLKDRRFPTQQELD
                     EAAPKHPVMFRTGPDAALNSLALQLCGIDRNFEITDGKPGFVEKDPVTGEPTGILRSC
                     TRLVKASTSARSPTPEEHREALRKLLLAYNEVGLTSITDRGTTDRSIELFKQLLDRGE
                     LTCRMFLTYYVDAQEPLETIESKIKKVIAHPLHQYNPWIWLRGFKFYLDGGMLTGSAY
                     MRQPWGVSKIYGITDPEYRGVLFLEPEKLYQIVKLALANGLQPTAHSVGDGAVLALAE
                     AYARVNQDFPVKPLRPCICHANFMSPEAIDLMAKCGIVADLQPAWLYHDGATLRAHFG
                     EERLRYFQPYRTLAERGVIVGGGSDHMQKIGRRRSINIYDPWLGIWTVLTRLPRWSDS
                     PLHPEERITRQQAIQLYTINGAYLTFEEDKKGSLEPGKYADFIIIDRDVLSCPVDDIA
                     DTQVLETWVGGRKVYPQS"
     gene            26457..27554
                     /locus_tag="PLD05_05255"
     CDS             26457..27554
                     /locus_tag="PLD05_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76875.1"
                     /translation="MRAEFGNRSHFGFTLVELLVVIAIIGILIALLLPAVQAAREAAR
                     RSQCTNNLKQLAIAVHNYVDSYGVFPAKKQGTNGGADCTQHNGNYGSGWMRLLPFYEQ
                     KSLYDQWAAPQTFGTGLYPPFGPCPWGPSDQGYQIYFQQVPTLMCPSDPKIANKSATA
                     YGRSNYKFSVGDSVPHDGAVGNNESADTRGIFANLAARITFAAVVDGTSNTVMLSERL
                     FPADARASNQGIAYNLGSSQPPRDCYLTLDPANKKLYLSSLSVATWGSKWAHGATSQI
                     GFNTVLPPNGPACASLANDNTTYIILPPTSQHPGGVNVAMADASVRFVSDTVDTGNLS
                     QFWPTSHDAPSPYGVWGALGSKAGGEPVQGF"
BASE COUNT         6149 a         8518 c         7504 g         5588 t
ORIGIN      
        1 acgaacggta gcatcaaaaa tggcctccac gcgacagcta ggccctcccc cggcaaaata
       61 gccaacgacc tgggctttaa acacactacc gccgcatgtc acaaagggca ttaaggcctt
      121 catctcttcc aaagtcacca acccctccgt gagaagccag gtttcataat aataattctc
      181 atcatcaata aacgtgggat cggaagggcg agcctcaaga atcaaatccg caacacccgg
      241 atctaggttc ggtatcccgt aaaggattga cggcggtgcc aaattgatgt tgattcgacc
      301 tgggatgacc ttactcgatg tggccgtcaa attggccatg atcaccggca aaatcactcg
      361 gagcgccacc gggtcttccg gcacgacggc atccaacacc cgcggctcct gctccccggc
      421 aaattgcaca cggaccttgg ccccgatgag gtccagaacc gtatcaaact tgaccttagc
      481 ctggcgggaa aaatcgaggc ttgcgttcaa ggggacgggt tcgccttgtt cctctccggt
      541 atagggcccg ttctggcgat acgccacaat aaacgtcgcc aattccgggc caaattcggc
      601 ctcgatttcg tcgtacagtt tctccaggtc actctggttg atgtcgatct tgggagaacc
      661 atcagggcga acgttcagtt ccatgctgaa aagcgtcaaa taagccgacc acccacggta
      721 cgccacggga tcatccgcgc tgagattggc aaagacctcg gggatcgtct ctcccggatc
      781 gatgacgcca ttgcggttac ggtcggcccc aaacaatagc tccggcgtca caccacgtac
      841 aagcagcagt tcctcgatgg tttccagtgg cccattctta ggcgtgtaag gggtgggaag
      901 cgttgtgtaa tactccagct cggccccaaa ttcccgcggt tcgtcatcgg catcaagcca
      961 atcgaggatc gcatccgcta tatcctccgt catccccggt aaagccatga gcaactggcg
     1021 accaccattt tccacagttt tgtcggccaa aaggagactg ttgatattca gcttggccga
     1081 ttcgttctcc aggccatagc gaatcgccga ggccccatag tcgctccaat cgggcccgat
     1141 gattgaaaat aggctggttt cccggggatc agccccttcc tggacaacca ctcctgcaaa
     1201 ccacgacggg ttgttaaacc aaccgccgtt gtcgttttgc cattcctcgt ccatggagac
     1261 aaaaaaccta gcagcctcca cgcccgacat agcggcagca cgtgcctggg cgatctggac
     1321 ggcaaccttc accccctcac gttcggccaa catcaagtcg ctcaaggtaa gagcggccaa
     1381 ggaaagtatc gccaccgtga cgagaaccac caaaaggatc atgcccctct ggcctcggcg
     1441 gtccttccgc catccccgtc gcgtcgcgaa cactccgcag aaaaaccacg ggcgaccagc
     1501 accgctggca acaaccttcc tcacaggcgt gccctcagcc gcccggagcg cgattcgttg
     1561 ccaaacggac gccttggtca ttgccgcgga cctcctccaa aagccccgcc agagctacca
     1621 ccaggggtga aaccaccgga tggtccgcct cccatcggtc cgccaccaaa accaccgccg
     1681 cttggtccgc cacctcctgg gacaaacccg ctaggcggtc ccatcccacc gccggaccgc
     1741 cctccgccgg cacctgagcc gccaaatccg ccaccagggg tgaaaccacc ggaaggcctg
     1801 cctcctgtcg gcccaccaga tccaccaccg aagcccccgc caggcggtcc cccaccacca
     1861 cgcacaaagc cacccggagg ccccatccca ccgccggacc gtccaccacc gaaaccaccg
     1921 ccggggccaa aatcccctcc acccggtccg ccgaatccac cgcccccacg gtttccgcca
     1981 aaaccgccac ctgggccacc tcctgggccg aacccaccag acgatgggcc ttcaccctgc
     2041 cggggacctg gacggccgaa atcaccacca ccccgctcac cgcgaccacc ccaaccaccg
     2101 cttcggtcac ctccgcggtc tcgctcgccc tgaccacctt ggcccggacc gccgccaaag
     2161 tctcccccag gaccgggtcc catgccgggg ccctcctcat cagactcctc gggcatctgt
     2221 tccggcggag gttcctcctc tggtgccatc tcctcttcgc cttccgagac cgtctcctcc
     2281 tccgattcgc tacccgatgt cgctccaagg agtcctgtct cgtgtgccgc tggcagatag
     2341 accaccgtcc gataaacgcg gcaattgctc aaatcgtcca ataaacccaa cgataagctc
     2401 ccgctgtccg ttgtcacatc ggcaacgggc gaaagcccgg agagaatccc cccgttttga
     2461 gattgactcc cttgcgaaga ccgaatgtac aatcgaatct ccaccgcctt gggaagcgat
     2521 ccttgggcgt ccgaatccca cgaatcacac catgcgctcc cgtcgaaata ggcaaagcta
     2581 atcgcggcga cttctgaggc gatcggttga agttgctgat ccacggcctc cagctctccc
     2641 gtcatggctg cgtaagccgt gatggcccga tcaagttccc gccgcaccaa tcccccctgc
     2701 tggtcaaacc caacgactcc cggcatgctc acgaacgagt agttgatggt ctcctccggc
     2761 gagaggacga aatacgcaac cgtcttcacg tcgctgatgc ggtccaccaa aagaccatcc
     2821 gccgcgaggg catattcgga ttgccattga tcgggccggg gcaaacggct aacgtcaatc
     2881 tcgatccagt ttgagccccc atataacccc gggacagaac gtggcagcgt tgttgatgtc
     2941 cagtcgcctc ccgtactata tgtccccatc tcgttacccg attcaagaag cagactggga
     3001 tcaattccag cctcctcggc cagcgaggaa aggttgctac tgctccccga ctttgtgacc
     3061 agacccggga tcaattcctc aaaattgaca ggatcatacg agaccgcccc gcggatatcc
     3121 tgggcaatcc gatcgagcag gaccctcgcc aattgtgtct cttccacctg atgccgggca
     3181 gactgaacca cccgataatg aaaatccacc gccgtgccca gggcagccaa caagatggcg
     3241 attaaaccga gtgaaaggag tacctccaag agcgtcagac cacgggtgcg gcgcaacttc
     3301 cgagccaggg cgagaaaacc accgctttgc cccgctcccc agggcgcgat gctcattccc
     3361 gctttgcttc cgaagctgat tgtctgtcgc ttggtaagat tcatatgtcg ccttccgtag
     3421 agtctgatgg tcacacctca cccggctcca ccaagagcct attccatact ctcccctcgc
     3481 ccgttcggcg ggagaggggt cgggggtgag ggtggctcga atcgccgccg gttttctcga
     3541 tcacctccga cgatcgctag ccctcacccc aaccctctcc cggcgggaga gggggactat
     3601 ttgagacagg ctctaaatac cgttctgccc gtcctgcccg ttcgttcccc gcatttttac
     3661 cgtgagcccg tgccttcctg gcctgtcgat tcttccgtcg aagcagattc cgaagtcgcg
     3721 gagctttcca tgccctcgct ccccgtcttt tcacccggct gcacgaactc cacggtgggg
     3781 tcaatcatcc attgggtcag cgtcagttca acaggtcgag caaacaggga cgggtcctga
     3841 taaaccgtca cccgaacgct caaaagcccg ttggtgtcaa gttccccgat gtcaattgag
     3901 taaagccagc ccggctcaac cgtgtcgtca atatcttcca ccggtgcatc ggcaatcgtt
     3961 tcaagcggca aggaaccggc gttcagctcg gcgagaatcc cttcgcaaat caattgggcc
     4021 cgcgtcaggt ttttgacccg cagggcgttc tggtacgcat tgaacacaag ctgcccaatc
     4081 gccgccagcg aggtaagcaa aatggccagc gcgagaagga cttccaacag cgtgaaccca
     4141 tcccgccgat ggcgatcggg tttgttgctg gtcggaagtt ttttctcaca tgtgaacatg
     4201 acaacacttc tgccgatcgt gggcccgaag ctcatcccaa cccagcgaat ggcaaccaca
     4261 ctatgatccc gccaccgacg gggcggcggg cagttccgtc atcccggccg attcagagtc
     4321 atacaactca ccgaccagga cggccccggt caagccacgc aaagaaatgt caatccgtcg
     4381 gccaaactca ttctccaacg tgatcagggc cgaactcgtt gttccatctg ggaaaaacag
     4441 gatcgacgac ccgtccgatg gtgggctcga gagcgttgga tcctccgtga tagccgccgt
     4501 tagctccccg ccttccacct gaacatccac gaatgtgatt ttttccggaa gctctttccg
     4561 cgaaccacca cccgggattt gcatcagcgg cacatcggtc atgtcttgag tccccatgga
     4621 agggtcataa acatccgtcc cgggcaccga ggcatcgtag gggacaagga cgtaaccgtt
     4681 gccgcccaat tcacactgaa agtaatacgt ctgaccattg gccatcgcac gatttctggc
     4741 ccggatccat tcggcccgga tttgatctgc ggccagttgc aggcgtcgtg ccgccatggg
     4801 acgcatcagg gcattgaaag ctacgcccga caggatcacc accaggacga ggacaatgac
     4861 cacctcgagg agggtaaagg caagacgggg gacatcgccg caggcccgct tagaccgacc
     4921 aggcgggaca acggtccatt gcaccttccg cgcggcatag gtccgcccga acaccaaact
     4981 catcgagact tgtcccctcc gctcccaaaa cgcgtcttaa caccatgtgg cttactgacc
     5041 ccaattggtg atgtcgtcct cggtgccgtc aacaccatcc ggccccattg accaaatgtc
     5101 cggcttatcg gtgttatatt gcccaggata gaggtactgg tattcccgac cccacggatc
     5161 aaggggaacg gcttgggcgt ccaagtaggg accgttccat ttggtggggt cggcgaggtc
     5221 cgctggcggc gtaatgaggg catcaagcga cggcggatac tcattgaaat ctaggtaata
     5281 cgcatgaagg ggctgctcga atgccttaat ttgagtcctc gccgcgttga tgtacgccct
     5341 tcgctgcacc gcgatcaagt tcgtcacggc cagcgaggca ataatgagca ggatcgtaag
     5401 gacgatcaac acctcaatca gcgtcaaacc tttgcgagtg gtccgcgaca gtttctttcg
     5461 cacaaagcct tttgtctgtc tcatcttccc atccttttgt cgttgccatc ccattcgtgt
     5521 ggtcggtgcc aatcacccaa tcggcgggga tcgccgccgt ggtctgggcg tgacacttcc
     5581 ctatcgtata aacaatccaa aaccgcttga ggtaccattt tccatacaac gattttttca
     5641 gagggaattt ggcagcaagt ccgctgccaa aaagcctggc aggttttgcg aataagagcc
     5701 tatcccagaa tctcgcccca cccgttcggc ggggaaggtc gggggtaagg atggctcgaa
     5761 tcgctgctgg tcttctcaag cgattccgac gttcgcttgc cctcactcca accctctccc
     5821 ggggggagag ggagcttttt cggatagact ctaaaacgac tggcctgatt tacactcctt
     5881 taggtgggac gtttgtatcg ctcgggtggt tagtcaccca ctgcgggtca acaccggggt
     5941 gcggcgagtt aacctcattt tacccgacag caccgctcat cttaaacacc ggtaacagca
     6001 gcgccacgac cacgaccagc accgccatgg ccaccagcag caacaggatg ggctccaaca
     6061 agcgaacccc cacatccagt tgtcgccacg tccgccgctc caatgaatcg gcaatttgca
     6121 agaggacggt ctccaacgta ttcgcctcct cggccacggc gatcatttcc accactaagg
     6181 ggggaaagag tttgcacgcc gccagcggtt ccgccaggcg ctgaccttgt gaaatgtttt
     6241 tggtggcttt gtgaaccgcc tctcccagca ggacgtttcc cgtggcttca ctggcaattt
     6301 ctaacgcccg caggatgggc acaccatttt tcaacagggt ccccaaaact cggcaaaatc
     6361 gggctaccgc aaagttgagc acaatcccac ccagaatcgg gaccttcaga acataacgat
     6421 cccacaaccg ccgccccgtc tcgctcctca gccaagccct cacactgaag aagcccaaga
     6481 tgatcacgac aacgacccag atgccccatg cctgaaggaa ttggctgaag gccagcagcc
     6541 cttctgtcag gcccggcaat tcattccgtt cccgcaggtt ctggaacatc ccctcaaact
     6601 ggggcacaag aaagatcaaa aggccgctga ccagcaatga cccgatcacg atgaggaaca
     6661 tcggataggc catagccccc ttaacccgtt ttcgcaggtc ttccgtcgcc tcggtaaact
     6721 cggccacacg gtagagggcc tcttcgagga acccaccttc gctccccgca cggaccatac
     6781 tgctggccat ctcgctgaag acacccggaa accgggccat ggcatcacca aggcccatac
     6841 catcctcaac gcgccgggca acttccccga gaacctcctt gagtgtcggg tgaaccgact
     6901 gacttttcac aatgttcaag gcacggagaa ggggcacgcc gcttcgcaga aggtcggcca
     6961 gttgaccata aacgacagcc attgcctggg caggaacaga tcgtcggcca aagctgaaaa
     7021 gaggccgcgc gggggcctcg cttcgcacct ccaacgggta aagctcccga ctggcgatgg
     7081 ccgtcagggc ctcccgtttt gtggcggctg cgattgtccc cgtcacccgc tggccattcg
     7141 catcaacggc gacgtaactg aactcaggca ttttgggcgg ccgctatgat catctttgac
     7201 cgggttctat ctcgcggtga ctggtaccga ggggagggcc atgggttaat gctcttcggc
     7261 ccgcgtcacc cgctcgatct cttcaattgt agttcgccca ctcaatactt tccgccatgc
     7321 gtcctgccgc agggtgatca tcccctcttc gatcgctgtc tgccggatca cccaactgct
     7381 agcccggtcg tgagccagtt cccgaatcct ttcgctggtg acaagaagct cgaaaatacc
     7441 cacccggcct cggtaaccca cgtgccggca cgcccgacat cccaccgccc gatataacgt
     7501 gacgccctgc tcgaggattt cccgcgggaa gtccttgggc aattcttccg gtcgtggccg
     7561 atagggttcc ttgcattcgg ggcaaagtgt cctgaccaat cgttgggcca tgacggcctc
     7621 aaccgtgctg gagaccaaaa acggctccac gcccatatcc accaaacggg tatatgcccc
     7681 ggcagcatcg ttggtgtgca aggtgctaaa caccaggtgg ccggtcaacg acgcctgaat
     7741 cgcgttttcg gccgtctcca ggtcacggat ttcgcccacg aggatgacat ccgggtcgtg
     7801 acgcaagatg ctgcgaagcg aatgggcaaa ggtcagtccg atcttgggat ggacctgaat
     7861 ctggttgatc ccttccaact ggtactcgac agggtcttcc gtggtgatga tcttgatcga
     7921 ttcatcccta atttcaagca aggcactgta aagggtcgtg gtcttacccg acccggtcgg
     7981 ccctgtcacg agcaaaatgc cgtgtggcag ttggatcagc tcacggaatt tctcgtaaac
     8041 atcttgctcc atccccagtt tccggaggtc gaactccatc gcgcccttat ccagcaaacg
     8101 aaggacgatc ccttcgccgt ggatcatggg gatgacggag acacgaacgt caacctcgcg
     8161 tccggccacg cggagttgga tgcgcccgtc ctgcggcaac cgccgctcgg caatattcag
     8221 ccttgccatg atcttgaggc ggctgatgat agcggcgtgg aaccggttga tctcgggcgg
     8281 gactggctga ggatgcaaaa cgccgtcaat ccggtaacga atcttgagcc cagacggctg
     8341 cgattcaagg tgcacgtcac tcgctcgcag atcgatcgcc tctaacaata tttcattgac
     8401 caatcgaacc accgagggtt cctgggccaa cttcgatagc tcggaaccat cagtttcgat
     8461 ctcgtcgagg agctcaacag cgctttcttc ttcctccccc tgagccatca actcctcgat
     8521 ggtctcaccg cccacaccca agtaggtctt gatgagcttg gcaatctcgg cctggctggc
     8581 caggacaggg acgaccgttt tccctgtggc ggtggccaat tcgtcgaggg ggtaaagatt
     8641 gaacggatca ctggtcgcaa cgagcagtcg gccattctct tcccggatag gaaagagcga
     8701 ctcccggtag atgaatttca cagggaaatt cttgaggatg ctgggatcaa tggtggcctc
     8761 ggaaagatca atgtactcga tccccaattc ctcaccaagc gcgcgaaggg cgtcctcctc
     8821 ggagaccagc cccatcccca ccgcagcctg atccagtcgc ttaccctcag tctgatggcg
     8881 tcgggccaac tggagctgct gcggggtaag aagacctttg cgaaccaaaa tttctccggc
     8941 ttccataatc acgctatcat tcccattttg gtgacgccaa ccgtcaaatt ttgcccgaat
     9001 tacgccaccg acaaacgctg cccacgaagg ccaaacctaa aaaacagtca cctggatcaa
     9061 aagctactga tctcctgtct cgcaccgctc aaatcaaaac cttggcttgg cgaaacaaac
     9121 ctatcgttat cagggaggtt atttttctgg atggcccgat cggaaagatg caaacactta
     9181 acgcgggaga gttcagccga taccccacac tcattccatt gcatgagagc gctgaaccgc
     9241 acgtcccaaa actctgtcaa cacattataa gagcaatcaa ggcgtgacca aatagttaac
     9301 acatttcgtt gacaaaaggc tacaaataaa gacgctggac tgcaaatact cccaacatca
     9361 ttaaaggagc ccccgattcc gtggggattt attgcccgcg aacagcgtaa tgttctagca
     9421 taatttgcgg gaatcggggg ctttccttgt gatggagccg acgcagcggg acaggcccgc
     9481 gggggcaatc tcaggcgccc cggtttccgc cacggcgctg accaccagga gcgccgcccg
     9541 gtgccccgcc aggaccgccg cggaagccac cgaacaattc ggagcgatcc agttcaaact
     9601 tttcgccgat catgctcttc catttgttct tttgttcttc cgtcaactgg ctcatgacgg
     9661 cctcttccat cttctgctgc atctgctgca tccgctctcg catctgctca gggcttggac
     9721 gctcaccctg ttgccgctgg ccgcgcaact cttccatgac cttctggacg gcgtcacgga
     9781 tcttttgggc ctgctcctcg gtcacgccca attctttccg gacttcctgg tccatcatgg
     9841 ccgcgccgat tccgatgacc tgcagacgaa tctccttgag tcgcttgact tgatcgggaa
     9901 gcagaacgcc ttcaatggct ttctgggttt cctgctgggc ctgttggaac cgctgccgag
     9961 cagcctctcg atcttgtccg cctccctgga acaattcccg catcctttcc cgcgacttct
    10021 caaggatttt ctctacctca gccttctgat catccgtcat ttcgatctct ttttggacct
    10081 tttcaatgcg aaggagctgc gcgtaaccca cgccgccacc cgggcccatc atgaacatgc
    10141 cggggccgcg gccaccgggc ccaccaggcc ctcccggtgg ttgggccatc gccgtgcccg
    10201 ccaccaacgc gaatgccatg cccaccactg tcacaaggct caagacatac ttcctcatga
    10261 gacgacctcc gaacaggaaa caccaaacac ctcaaacacg atccgaaaga ccggtattca
    10321 tgaaaagcga accggtcaga aggtgtccac caccctccaa gagatttaac agcggtgggg
    10381 gcagaaagtt ccgcggcgga accataaacc ttttttcaga ccccgctggc gatggccttg
    10441 gcaacgtcgg tgcgataggc taccaacgcc ggcaggtaac ccaccacgga ggcgagaata
    10501 atcaacccgg gcaccaggat tagctccacc gtttgaaagc tgagggcact aacccgcaca
    10561 ccggtctctt ctaaaatgac ccccgagaac gcccctatta ggccatgtcc caacagaacg
    10621 ccaatggccc cacctcctag cgatagaagt atcgactcaa ggaggataac cgccattacg
    10681 gtgctcctct tcgcccctag tgcccgcatg atggcgatct ccagtttgcg ttcgttcatc
    10741 gagttgtaaa tgctcaccat taggccaatg cctgctacga ccacaataag aactgcaaaa
    10801 attagcaaaa tcgtctggat gtttcccagc aatccctcaa agagcttggc cacctcttcg
    10861 gtggggatga cgctttgaac cccaggctcc ttgctcacct gcatgggcaa agccagtgcc
    10921 ttggccgagt tcgtccagtc ccgcttaacc aggacggccg tcaattcgcg atgattctcg
    10981 tcatgagcct cggtctcgct cgtcgcctcg tctccatctc cgtccatcgg gttaacgctt
    11041 tcttcctctt cggaggcctt accctgccgt gcatcgtgtt gatggagcgt ccagaagcct
    11101 tcgatattga caaacaaggc ccgatcgttc ggggtccccg tgggctttaa aacccccaca
    11161 atcgtaaatt catcgggatg ctcgtggcct ccttgttcgg ccaggccgtg ggtcacgcgg
    11221 aacgtgtccc ccacccgcag gcccgccctc ctagccacgg tcgcgccgat cactgcttcg
    11281 aaaggtgcgt cgggcgagaa attccgtccg cccggctcaa actcgtaagg ccgatccccc
    11341 agataacgga actcctcaaa aagctccggg acggtcccca ccacagggaa gccggcataa
    11401 cttccgccaa tacaaacggg gatggcagcc gccacctcgg tagcaaagcg tccgtgtgaa
    11461 agttcttcat aaatctcata aggaatggtt ccccgcggcg cctgcaagta atagacggtg
    11521 ctcaggacaa tctctagagg actgcctttc gcgggaccga cgatcatatc gtacccctgg
    11581 gaactccgcc ggaaagaccg gtctaacacg tgatagatga ccagcacagc aaccaccagg
    11641 gccaccccca gggccatgga aaaggccgtc aaagccgagg ccaccgcgcg atgttgaata
    11701 ctccgccagg ctatcttcca aagaggcatc ttatcaggac cctatgacgt tctttgatgg
    11761 cacaactaag ctaattgacc gttttcgact gttaggcgta acatcatttc caaacgtcac
    11821 ttccgtgtca ggaccaagtt gatgtcgggg aggcgaacga tgcggggaaa ctgtcgggca
    11881 acctccgggc tatgcgtcac caggatcaac gccacgttct cttcccggca cccctcccga
    11941 agaaggtcta acaccgcctg ctgattaccc gaatcaacgt tggccgtcgg ttcatcggct
    12001 aagatcagtt ttggccggtt ggctagggca cgggcgacag atacccgctg ctgttccccc
    12061 acggatagtt gccgtggctt gtgatgcatg cgatgcgcca accccacgcg ttccaaaagg
    12121 tgtttggcgc gggcaacgtc gggttttccc cgaccaaacg tcatccccag gaggacattc
    12181 tccaaggccg tgaaggccgg aagaaggtta aacgtttgaa acacgtagcc gatgttttcc
    12241 gcccggaaac gatcgcactc agcctccgat aggagggtaa tatcccaccc attgatgcga
    12301 acctgcccct catccggtcg gctaatcccc gcaataatgt ggaggagcgt ggtctttccg
    12361 cagccacttt ctccgacaat caccacctgc tcgcccgcct ctaccgtgaa ctccgggata
    12421 tcaaggatgg gtagttcacc gccgtccggc tcttgaaacg acttgcgaac atcacgcagc
    12481 accaagagcg gtgattcgtc ttgtgggaat accatggccg accagactta tgtcaaaaga
    12541 aacagacaca aatacaacgt attttcccag ggcttgagtt cgatctgatt gcaaatgcta
    12601 cgtccgcgga tagaccccag ttcgctcatt gtacacgccc acgccgtcca gcggggttat
    12661 ttggcgcggg taaaggtatc cgtgatgagg gtcacaaggc cgggcgctgg taactgttaa
    12721 gctgcacgca gttcccacat cgaaccggca aacaagcggc ggtaaagttc gcactgttca
    12781 agaagctcat gatgttttcc caagcccaca atttggccgc cgctgagaag aaggatccgg
    12841 tctgccatgg cgatggtatt tagccggtgc gtgacgatga tggtggtccg tccttgggcg
    12901 aaccgttcca gggcctgatg gatgagctgt tcactttcca catcaatttg actggtggct
    12961 tcatcaagga tgaggatggc cggatccctt aacatggccc tggccagggc gatccgctgg
    13021 cgctgccctc cagagagtcc gcaccccatt ggcccgagaa gcgtgttgta cccttgggga
    13081 agctgctcga taaacgtgtg ggcatgggcg gcgcgggcag cctcgatcgc ctgctcgtcc
    13141 gtcgcttgtg gcgaaccgta tttgatgttg ttcaggatcg tgtcgttgaa gaggaccggc
    13201 tcctgcggga ccaggccaat ctgttttcgc aaagagcgca agcgaatatc gggaaggggc
    13261 acaccatcta agcgaatttc ccctcgattg gggtcaaaga accggggaat gagactgagc
    13321 aaggtactct tcccgctgcc acttgctccc acaatgacga tcgtttcacc aaacgggatt
    13381 tccaaattta tgtcgcggag aacgggctga tcgggcgtgt aggagaacca cacgtgatcg
    13441 aagaccaaat cccgccggtg acgaggaatt cgccgagcca aagggcggtc cttgatctcg
    13501 ggttcgcgct ccagcatggc aaacacacgg tcagaagccg ccatggccgc ctgaaggcta
    13561 gcgaaaatat cggaaagtcg tctcgccggg tccgccgcgc caacaagcag cgcataaaac
    13621 agaaccaatt ccggcatcgt caaggcacgg ctggagacgg tcaagccaag cagatgcgtc
    13681 ttctcgttca gaacgagata cgcaccggcc gccatggcaa ggctgataat cgacattcct
    13741 aagatttctg ttaggggatg cgtgagggca tcaaaaacgg cgatccgcat gcccttgttc
    13801 agatagcgac ggctcgtctc atgaaagcgc cgcctttcgt acggttccat ggtgtaggcc
    13861 ttcaccacgc gcagcgatct taaggcctct tccaatgtac cgtaaagttg ggccatttct
    13921 tccatggccc ggcggttggc ccgtttcagg agcttcccca atttccagat cgcccaggcg
    13981 gcaggcggaa cgaccaagag tgaaaaaacc agcagcggaa aacagaccca ggccgccccc
    14041 actaaacagg ccaacatctt aagcggttcg cggaccatct tccctaagag aacggaaaga
    14101 cctgaataaa tgcaattgat gtcgtgcgtg aagcgactca tgaggtgggc cacaccttca
    14161 tcacccggct tactcaattc cagccgcaga acatgcgcga agaaccgacg gcgaagctca
    14221 aagaccgttt ggtgggcgac gcgggcaaca aggaccgaat tgatcaccag cgccacactt
    14281 ttcagcacgg tcccaattag cagcaagctc aaaatgaaaa ggagcgtgga aaacgcatcg
    14341 tttggcgtga ggcgaagaat ccacggccgc agccacagat accaatctcg cttaaccagc
    14401 agttgcgaaa gttgccccga tcggaccggt cgatgcgatg tgccggcctc acggtccgac
    14461 ccatcggccg agggtccgtc cgcttccgtg ccccccgcgg cggcgatctc ctcttgcagc
    14521 cgggcgatct cctcgtcgca ttcctgaatc ttcaaatcaa tccaatagtg gagtgactct
    14581 ccgcgaaagg ccacctctac aatcgggtag atcgctgtga tgtttcctcc ccacatgacg
    14641 ccaacaaaca gggccgtgac ggcaatcgcg acaatccata aggggcggcg gagagcgatt
    14701 tggacaactt gaagaaaatg gttcatcctt gaacactgac tccgtcgtct tcaaaaacca
    14761 agctcgtccg aaagatgcca ggcaagcagg caagcaatcg agagtctatc ccaaaaaagc
    14821 cccctctccc gccgaccggg cgaggcgaga ttatgggata ggctctaacc cgcgcgaaaa
    14881 aacacacggc tttcgttcgg gctaacgcct tttttaccaa atctttgcag ctttacaaag
    14941 atcaatccga gaccagataa acccccgtcg gccattctgg agggacgcca tctgggaggg
    15001 acggggtccc ccgcgtcctt taaaaaactc ctaccgtttg cgacgaacgc gctctgtcgc
    15061 gtccgggcgt ctcatggcga ccgacggggt cccggtttag aaaaagcttt ccacaatctg
    15121 acgaacctct tccaaccagc cgacgcgctg ctcgtaggtg ctcatgacga tcgactcgaa
    15181 attacgtcgc cggaaatggg tgagaccaca gaaaccaaaa atgcagttct tccacaaatt
    15241 ctccaagggg tcgccgtaaa gccggagttc gtcttcgcgg ggtgtgttgg aggtcgtaaa
    15301 gacgagtgcc cgcttcccct tcaaatgccc aacaacgccc tctgccgaga aatcatacgc
    15361 cacgccctgg cgaaagaccc ggtcgatcca ccccttgagg atcgcaggcg gttgtgccca
    15421 ccaattagga tggacaatca cgtatccgtc ggcctctcga agctggcggc agtgttcctc
    15481 gatctccggt ggaacggttg catctttggg aatctgatcc gcgggcaaca gaggatcgaa
    15541 ccgttcctga tacaaatcgt ggaagatgac ctcgtggttc ttgctttgga gagacttgac
    15601 cgccgtttcg gcgatggcat ggcaaaaact gcccggtcgc ggatgcccaa tgacaacgag
    15661 gaccttcatc gctttcctcc gtaaggacgt tgcctcactc aacttgttga tcgatcacac
    15721 ctcttgatcc tcaccacaac ggtcagccga ctccgccatc acgatttaac gagcgaaagc
    15781 tctcccggtc gatagggtgg cggatcgcct tccggcaact tgaacgaggc gaccacctcc
    15841 ggatgaatcc gcgtcggttg acccgtggca tacgtcacaa acgcccaaag ggtttccgcc
    15901 tgggccaaca aacacccatc gttaacccga taaatccgat agcgccggcg agaggtgacc
    15961 tttttgaaat cggcaaccca cgtttgcacg acaatctcgt caccgagcct cgcagggtga
    16021 atgtactcga tcgtgtgaga cctaaccacc caaccggccc caagggccag ataacgctcc
    16081 ggtggccaac cgcgggcggc agaatgagcc accgcggcgt cttgcaacca ctgcacatac
    16141 accacgttat tggcgtggtt cagcgggtcg atctcgctgg gctgaaccgt atggacataa
    16201 taaaacagaa ccggcatagg tcacgcactt ctcggcaaac cccattcgga acgcttaaac
    16261 ccacaaaacc cctatttcct taaaagtcta tcccaaaaag tccccctctc cctctgggag
    16321 agggttgggg tgagggcaag ccaacgtcgg aatcaattga ggaaaccggc ggcgattcga
    16381 gccactttca cccccgaccc ctctcccgcc gaacgcgtgg gggaagatta tgggataggc
    16441 tctacactgg atcattttct acgctcaccg gcgcgataaa gtttacccgc gatgccaagc
    16501 cagctcagca ctgcggcaca caccgttgcc cattttcttc agcttgaggt gcttttttca
    16561 tcgacggtga gcccttatcg ccaaaggtcg tcatcggcaa agggatggag cggaccggac
    16621 gcccgcggct tcggcaaaat cttgagaatc tgttcggcca gacgcaagtc atccttagtt
    16681 gtgatcttga tattgagtgg cgaacccgac acgacataca cggcctttcc caggcgttcg
    16741 actaactgcg catcgtccgt ggggacaaag tcgcctcgct tttggtaggc ctcaagcagc
    16801 cagtcccgcc gaaagacctg gggcgtttgg gcctcccaca acttgtcccg ggaacgagtc
    16861 tcgacgatct ttccgctgtc atcgaccttt ttcagtgtcg ccgtcacggg aacggcaagg
    16921 atcgccgccc cggtttccac cgccttttga aaaacggcgt caatccaaag gtcgctgatg
    16981 cacggtcggg cggcatcatg aatgcaaaca aaatctatct cctcgcgaac ccgcgttaag
    17041 gcgttagaaa cggtgtccac ccgttcgcgc ccaccgtcca ccacctcaac acccaacaag
    17101 gcgatgtcgg cgccaaaacg tcggaggaac tcttcccgat cgtcgggcgc gatggccacg
    17161 atgagttgct ttacatcgtc ccgctgggta aagcgatgga gcgaatgcag ccacacagcg
    17221 cggcccgcaa gtggcgcaaa cggctttttg taatgaggat cacgaaaacg tgaactggca
    17281 cccgcggcgg gcaaaatcac ggcaaatcgc ggcacgaaaa cgcctcctca aaccatctcg
    17341 aaaacaccca tcaaccagat gcccggtcat gagtcgctca gattaccgtc aaccctctta
    17401 gacccaagcc gggactggtc cttgactcat gataagacat gggaccgcct ttctcctaga
    17461 gaacccgcgg aaaatgcgct gtcgaagacc gggggcggtg gcccgggaag gcgtaagagt
    17521 ctatcccaaa aagaccccct ctccctccgg gagagggttg gggtgagggc aagcggtggt
    17581 cggaatcgat cgagaaaacc ggcagcgact cgagccaccc tcacccccga cccccctccc
    17641 gccaaacggg cgaggggaga tcatgggata ggctctaagt cggtgtcggc caacaagcca
    17701 ctccaggcgg ggtttcagat ttcctcacgc cttgctgcac aaagccgttg ccgtcgcact
    17761 cgttttgcgg taaattaaag ggtgctggtg ggatgaacac tcggaagaat ccccaaacgg
    17821 taacgattcc ggggcgtggc gcagcctgga ttagcgcgct tgcttggggt gcaagaggtc
    17881 accggttcaa atccggtcgc cccgattcgt cggccgatcg acgcatctgt cgatcggcct
    17941 ttctcgtttc ttgtcttgac ggttgcccgt gccagggaat gaaaaacgca tagccgctcg
    18001 ccgaacactc ctgccttccg gtggcagcgt gcgattgcag cggggtctca cgattcgtgc
    18061 agagatcggg cttgtccttc gccatgaacc tcgagtacga agcgcacaat gggttgggtt
    18121 gagcgacgcg ttgcctacag tctgaagcgg gcgcgacacc gcatggcccg ccatgacgag
    18181 acagtccatt ggtggtgatc acccttgggg cagcctcgca ttgagcctgc caggaacgga
    18241 tttcgtcaaa taaaggtaac taaactagtg tagtattatt acttgtcttt tccgcctgat
    18301 cgcgtaaaat gaccggtgaa gtcgatcgcg tatcagaaca tttaataaac gcgatcaggt
    18361 aagtaattta taggatcggt ttgtttacgg gttactatcc atttttgaag gagcctcaaa
    18421 ccatgcttca ccaacagatt gactgggaat tggccgaacg cgaggcctgg gcattgcgac
    18481 ggcagaaggg tatccccaac aatcgtcggc tctttgtcct agcgtgcatg gacgagcggc
    18541 tacccgtgga ggacgccctc ggcatccgcg tcggtgatgc ccatgtcttt cgcaacgccg
    18601 gtggcctggt caccgatgat gcgatccgct cggccatgtt gaccacccaa ttctttggca
    18661 ccaaagaaat catcgtggtc aatcacaccg aatgcggcat gatgaccgcg acgggtgact
    18721 atctgacgaa cctcctgcgg gaaaaaggga tcgatcctga tgccgtgcag gttgacccgg
    18781 ccttgcccga gctgaaactc ccgcccggta cttttcccaa gtggatccgg accttcaccg
    18841 atgtcgatga aatttgcttg cgacaggtcg agcttttgcg aagctcaccc ttgatccccg
    18901 ccgatgtcga gatccatggt tacgtttggg aagtcgagtc gatgcgattg cgtcggccgg
    18961 gcgtccggct ggccgaaaag gtaaacaccg ccaaggccat gggggcgcgc ggttaaccgg
    19021 tctggtccgc tcgccgcaag atcgctcgct tcccaggtgt aacgccgggt cgagagttca
    19081 tccccctgac cgtcaagtgt cgggaaaggg tggacaataa acaccaccgc ggaaaaacgc
    19141 gcggaggccg ggacccgcct tcctcgcaaa tggtgggacg tttccaacgg caggacgcgg
    19201 ggaaccgcgt ccctcccact gatgttacgt ctgctgcaac cggcgaagga tggcctcccg
    19261 gtcgaaaacg catccggccc aggggccgcc gctgatatgt tggagcatcg cccaaaggcg
    19321 ggtatcctcc ggcaggtgct cgtctggtcg caactcggga tgcggggtcc gcgacttgag
    19381 gacgtgttcg gcctcttcgg gcgagaactc acgctcggca tcacccacca gattgatact
    19441 gccctgcaag gcccgggtgt cgatcacaat ccgaatccgg tcgccatcgc ggaccttggc
    19501 aatcggacca ccggtaaggg cctcgggcgt gacccagccg atgcacgccc cggtactcac
    19561 gcccgagaat cgggcatcgg tgatgacggc cacttcatgt ccccacggta ggtctctcag
    19621 ggcggaggtg atttggtagg tttcctccat gccggacccc atcgggccgc gaccaatcaa
    19681 gaccacgaca tcgccgggct gcaccggatg cgacgtttga cccttgatgg cggcgacggc
    19741 atctttttcg cggacaaaga ccctcgcccg caggacacga tcgaagaccc cgttgtcatc
    19801 gaggacacgg cggtggatcg ccgtgctttt gaccacggat ccgccgggtg ccaaattgcc
    19861 ccgcggaaaa catgatgtgc ctgtcatccc caaggccacc gcccgatttg gcgaaagaat
    19921 gacctcgtcc ggatcaatgc ctgtctcacg ccgcaagagt tcacgtagcc gatgcctccg
    19981 cggcgatcgt tcccaccaat cgaggatttc ccccaagcac cggcccgtta cggtaagagc
    20041 atccaaacgg agcagtccca ggtctcgcaa atggagcata acttcgggga ccccgccggc
    20101 caaataaacg tacaaggtgg ggtggttctt gggaccatta ggtaacacat cgacaagtcg
    20161 cggcacctgc cggttaacgc gttcccagtc ttccaaactg gggcgaggaa ggcccgtctc
    20221 gtagagcacg gcgggaagat gcagaatcaa attcgtcgag cccccgaacg cggcatggag
    20281 caccatcgcg ttatgggcgg cagcctcggt caaaatcgcc ttccctgcga gacccttttt
    20341 cgcaagatac acgagggctt gggcggaccg tcgggcaaca tcgcgccaca agggttgacc
    20401 cgagggggcc agtgcgctgt gcggcaaggc aagccccaga gcctcggcaa tcacctgaga
    20461 cgtggccgcg gttcccagga attgacaccc acccccgggc gaggcgcagc tgtggcaagc
    20521 cgcggcttgg gcctcctcaa gcgtgatttc attttgggca tatcgggcac caatcgactg
    20581 gaccttggcg gtgtcctcac cgcgggtcgt gggaagcatc acgccgccgg ggacaatcac
    20641 cacgggcaaa tccctcagcc cggccagggc catcatcatg gcgggcaagc ccttatcgca
    20701 agtggcaatt cccaaaacgc cgactgccct gggaatcgac cgcgccagcc gccggaagac
    20761 gatggccgca tcgttgcggt aaggcaagct gtcaaacata ccgggtgtgc cctgtgtgcg
    20821 gccgtcgcat gggtcagagc aaaaccccgc gtagggcatt ccgcccagcc tacgaatctc
    20881 tttcgcggcc tcggctactg cctcatccag ctcaaagtgt cctgtgtgat agcccaaggc
    20941 gatgggtttt ccttcagaat cccgcaaccc tcccagcgtg ctcaggatga caaattgagg
    21001 ccgacgagtc tcggcggggt cccagcccat cccggcatcc tggctcatcc caaaaagatg
    21061 accactagga tcataccgca acatagtgtc gtcaaacggc agccgcccct caggacctgg
    21121 cccaaagacc tggaccggtt ccggcacatg atccgccgcc ttcagtgcgt catattcctc
    21181 aaaatcgcgg taaggatgac ctgatgccat gcacagcctc aacaactaca aagggacctt
    21241 agcatccttg cccggctcct cgcggacata tcgcgggacc aaatacccgg gcaggcgttt
    21301 ccgtagctct tcgatgattt tcttccctcg gtccacgggc acctcaaagt gatgggcccc
    21361 cagcacacgg tcaagctgat gaaggtaata cggcaagacc cgcaggccgg cgatccgctc
    21421 gtagagcgtc gccaagacgt cggggtcatc gtttaccccc tttaagagga cactctgaca
    21481 caagaggatg gcgccggaat ggcccaaccg agtaatggcc tccgccaccg cgtcgtcgat
    21541 ctcttgcgga tgattgatgt ggaatacgac aaccgaccgg aacggggcgc ctctgaaaag
    21601 ggcgatgagc tgcggcgtaa cccgctgggg gatcacaaca ggcagccgag tgtgaatccg
    21661 cacgcgctga atatgggcaa tctgcgaaaa caaggaaaac caagtcccca actgctggtc
    21721 gtctattagg agcggttcac ccccgctgag gacgacctca tgacaatctg gccgcgattg
    21781 gacatatcgg caaatggacg tgatataccc atccgacgtc gggggcgagc cttccacatc
    21841 ttgagggtta tttggcagtt tgcgactgcc ggtttggcgc ggtgaggacg tcggcggagt
    21901 gtcgggaaag tatcggcgga aacagtaccg gcaatggacg ggacaagcgc gagtcgctag
    21961 gataagaatt cgaccctcgt attttgccaa aacgccgggt agggggcatg ccgactgctc
    22021 ctggagcgga tcggtggaaa accccggttc cgcagccgtc tcaggcatgg taggcaagaa
    22081 ctgcctgagc accggatcgg ctgggttgcc tggtttgatt cgggacagca tgggccgggg
    22141 caagaggagt gggaaattcc ccatcgccgt gcgtgcctcg tcggccagct ccgggggaag
    22201 atcgaggaga gaacacagtt ccgcgggatc acgaatggcc tgggcaacgt ggatcttcca
    22261 caaaggcgtt ccctctccgc ccgtcgtcct ggagggttca gattgacaag cgatttgatt
    22321 tggcatggtt gatggcgctg ttggcgtcgt gttggacatg cgacagattc ggttcagaga
    22381 actggacgcc atccgctgcc cgatgccatt cagttgtttt cttgccgctt atggtgatca
    22441 tcgggctggg gtaaacggat tcctagcgaa ctacgaataa tatgggtgaa ataaagcgga
    22501 tcggtatgct cgtgacacca gccgacgcat cccgtttcga caaccgataa ccgtattgta
    22561 ggaagaactt tgacaagcga aaaagggtgt gacacgtggc gacctataat accagtgaat
    22621 ttcggaaagg acttaaggtc caattggacg gcgagcccta tttgatgatt gagtgtttct
    22681 tcgtgaagcc gggcaaggga caggcccttt acaagtgcaa gctcaagaat ctcatccgca
    22741 acacggtcat tgaccgcacg tacaaaagcg gcgacacgat tgaggcggcc gacattgagg
    22801 aaatcgacgc ccaattcctt taccggcagg gcgatcagtt cgtgttcatg gacaacaata
    22861 cctttgaaca gtacgagctg accaaggatc aggtggacga ggcatggaag tacctcaagg
    22921 agggcatgcc ctgcacgatg ctcctattca atggcaagcc gatttccgtc acgccaccca
    22981 atcatgtggt cctcaaagta gagtattgcg agccgggtgc ccgcggaaat acagccacca
    23041 atgtcaccaa gccatgcacg gtcgagaccg gtgctgaaat catcgtcccc gctttcgtgg
    23101 aaatcgggga ccttatcaag gtcgatacgc ggaccggcga gtacatcgag cgcgtcaagg
    23161 cggaatagcg gcggaacacc atttcatgag ctggggacga gcacctgatg attttcggcc
    23221 cacggccaat tgggaggtgc ttcggcttcg tgctgagtta ctgcgggtaa ttcgccagtt
    23281 tttcgatgag cgtcaattcc tggaggtcga gacgcccatc ctttcggccg acacggtggt
    23341 cgatcgccat ctcgatccct tctggacggc cgacgtgaat cgaccggcgg acattacgcc
    23401 gcaaacgcga ctctactttt tgcaaacctc tcccgagttc gggatgaagc gtcttttggc
    23461 ggcgggcagt cccaaggcga tctaccagat tagccgcgtt tttcgccgcg gagagtgtgg
    23521 cccccttcat aatcccgagt tcaccctggt ggagtggtat cgcgtaggcg acgattacca
    23581 cacggggatg gaccttctcg ccgagttgtg cgaagtcctc tttgggcggg gtcgtccgag
    23641 gatttatacc tacggtgagg tcttcacacg attcgttggc atcgatcctt acagcgccgc
    23701 tgccgaccaa ctacggcaaa gggcggaagc cttgggcgtt gcctatccag cctcactctc
    23761 tctggaggac cgcgacggct ggctcgatct gctttttact gagaaagttc agccacattt
    23821 gggacgggag cagcccgtca tcatctgcga ctatccggca agccaggcgg cactggccat
    23881 cgtgcggaag ggccccccgt ccgtggccga acgctttgag ctgttcgtca acggtatcga
    23941 actggccaac ggctatcatg agctgcgcga tccggaggaa ctgcgtcaac ggatcaccac
    24001 ggccaacgcc cagcgtacga gagagggcaa aagccctctt cccccaacga accggttact
    24061 tcaggctatg gaagccggat tgccggcctg tgtcggcgtc gcattagggt ttgatcgcgt
    24121 ggtgatgatt cgggcagggg ccaattcgat ccgcgaggtc ctggcatttc cctttgaccg
    24181 agcttagagc ctatcccaaa gttccctctc cctctgggag agggttcggg tgagcgcgag
    24241 cgaccgtcgg agtcgatcga gaaaaccgcc agcgattcga gccaccctca cccgcggccc
    24301 ctctcccgcc gaacggcaga ggggcggttg tgcgataggc tcttagatca aacggcctac
    24361 gattgcggat acacctttcg gccaccgacc caggtttcga gcacttgggt atcggcgatg
    24421 tcgtccaccg gacagctcag gacatcccga tcgatgatga taaaatcggc gtacttgccg
    24481 ggttcaagcg agcctttttt gtcttcctca aatgtcaagt aggcaccatt gatcgtgtaa
    24541 agctggatgg cctgctgccg cgtgatgcgt tcctcgggat ggagcggcga atcgctccag
    24601 cggggcaacc gggtaaggac ggtccagatt cccagccaag ggtcgtaaat attgatggac
    24661 cggcgtcgcc ctatcttttg catgtgatcg gacccaccgc ctacgatcac gccgcgctcg
    24721 gccagggttc ggtacggctg gaaatagcgg agccgctctt cgccaaaatg ggcgcggagc
    24781 gtggcgccgt cgtggtaaag ccaggcgggc tgaaggtcgg ccacaatacc acatttggcc
    24841 atgaggtcga tggcttccgg actcataaaa ttggcgtgac aaatgcatgg gcgaagcggt
    24901 ttaacgggga aatcctggtt gacccgcgcg taagcctcgg ccaaagccaa gaccgccccg
    24961 tcacccaccg aatgggccgt cggttgcaga ccattggcca aggccagttt gacgatttga
    25021 tagagctttt ccggttcgag gaagagcacc ccgcgatact ccggatcggt aatgccgtaa
    25081 atcttgctca cgccccaagg ctgccgcatg taggcactgc ccgtgagcat gccgccatcc
    25141 agatagaact tgaatccgcg aagccaaatc cagggattgt actgatgcag ggggtgggca
    25201 atgactttct tgattttcga ttcgattgtt tcaagcggtt cttgggcatc cacgtaatag
    25261 gttaaaaaca tacggcaagt cagctcaccg cgatccaaca actgcttgaa aagttcgatg
    25321 gaacggtcag tcgttccgcg gtctgtgata ctcgtcaagc ccacctcgtt atatgccagc
    25381 aacagcttcc gcaacgcctc ccgatgttcc tccggagtcg gtgatcgggc ggatgtgctg
    25441 gcctttacca gtcgcgtgca actccgcaag atgcccgtcg gttccccggt gacaggatcc
    25501 ttctcaacga agcccggctt accatccgtg atctcaaaat tgcggtcgat gccacacaat
    25561 tgcaaggcta gcgaattgag cgcggcgtcc ggtcccgtgc ggaacatgac gggatgtttg
    25621 ggagctgcct catccagttc ttgctgcgtc ggaaaacgcc ggtccttcag ccgggtgaca
    25681 aagacttgct ggacaacgat ccactttccc tcaggcacga ccgccgcccg ttgccgaatg
    25741 tacgcaagca cgtcggcgat cgtttccatg gtggggattt cgtgatcgaa ctcgtaaacc
    25801 gctgccccaa caggatgcga atgcgtgtcg atcaagccgg ggagcaccat cttggaccgc
    25861 aaatcgacca gacgggtctg atctccggcc agccgcatca cctcatcgtt gccaccgacg
    25921 gccacaatac gatcgccgcg aatggccatg gcctccacaa cacggaaatc cttgtccacg
    25981 gtgacgatcc ggccatgatg gaaaatccaa tccggtggtt cttccaccgt cccaccggcc
    26041 caggcaatca gcggcaggcc catcatcaac ccaatgacgg cgttccgaag ggggaagcga
    26101 acggtacaca tgaccatgct ccttttgctg gcttgcgatg tttcaccgag cttcggctaa
    26161 caatcgtgcg accgacaaaa accctcgttg gtttccttaa cgtttatcaa tgcgcggccg
    26221 cgggcaaagc tattataccc acttgtaagg ttatgccgag gtggctgctc cccccgattt
    26281 cggcttgcac gcaaagtcaa tgcggcaaaa aatcaaaaaa taatgcgatc ggtccagcta
    26341 ccaaattcag aaaacttgct gtcgcagtaa ctcagatgta taattgggcc gaggcgagct
    26401 ggggacggat ccagacggat ccaactttat ggttctttta aggtcggagg tgtgtcatgc
    26461 gcgcggaatt cggtaacaga agccatttcg gttttacgtt agtggaactg ctcgtggtaa
    26521 ttgcaatcat cgggatcctg attgcgttgc ttttgcccgc ggttcaggca gcgcgagagg
    26581 ctgcaaggcg gagtcagtgc acaaataatc tgaaacagtt ggcgatcgca gtgcacaatt
    26641 acgtcgatag ctatggggtg tttcccgcca agaagcaggg gacaaacggg ggggccgatt
    26701 gtacgcaaca taacggcaat tatggctctg gctggatgcg acttttgccc ttctatgagc
    26761 aaaaatcgct ttacgatcag tgggctgctc cccaaacgtt tggtacaggg ttgtatccac
    26821 cctttggacc ctgtccgtgg ggaccgtctg atcaaggcta tcagatttac tttcaacagg
    26881 tgccgaccct tatgtgccca tccgatccca agatcgccaa caagtcggca acggcttatg
    26941 gacgatcgaa ttacaagttc tccgtgggcg attcagtgcc gcatgacggc gcggtgggca
    27001 ataacgagag cgcggatact cgcggtatct ttgctaatct tgcggcaagg attacgttcg
    27061 ccgcagtagt tgacgggaca agcaacacag tcatgctctc ggagcgattg ttcccggctg
    27121 acgcgcgagc gagtaatcag gggatagcgt ataacttggg atcaagccaa ccgcctcgcg
    27181 attgctacct gacgctcgat cctgctaaca agaagctcta tttgtcttcc ctgtcagttg
    27241 cgacgtgggg tagcaagtgg gcccatggcg cgacctctca aatcggcttt aacactgtgc
    27301 ttcctccaaa tggccccgcg tgtgcatcgt tggccaacga taataccact tacatcattt
    27361 tgccaccgac aagccagcac cccggtggcg ttaatgtcgc tatggcggac gcctctgtcc
    27421 ggttcgttag tgataccgtg gatacgggga atctgtcaca attctggccc acgagtcatg
    27481 atgctcccag cccctacggg gtttgggggg ctttgggttc gaaggccggc ggagagcctg
    27541 tgcaggggtt ctgattggtc agcgattatg ttcggcgcga tgggaaaaag tggggtttct
    27601 gagggcaacg catagggacg atagagcgtc ttgaatggct tgaggaggga ctttcatgaa
    27661 tcaccctgtt gtcagaattg gcgttttttt gtcgcccgca ctgttgcttt tgttgtacac
    27721 agcgtgctcc aggcaaaggc caaaaaatca acctgaaac
//
LOCUS       DAOSUT010000039        27740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1129_length_27740_cov_9.925014, whole genome shotgun sequence.
ACCESSION   DAOSUT010000039 DAOSUT010000000
VERSION     DAOSUT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27740
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_27740_cov_9.925014"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..4383
                     /locus_tag="PLD05_05260"
     CDS             64..4383
                     /locus_tag="PLD05_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOP76876.1"
                     /translation="MADRLAADGRERRSFKAQSGGKAEDKDKELLGKLTANRLRVWDS
                     RQDFLTPSVENLQRVGGTTRQNFLVLDNKGRLQYFGQNQLAQADVRALAREFQKRGIV
                     LYPEWTPHETAFWDPMVETNDSGKAVVKVVVPDRATSWKLLAKGVTVDTRVGESEAEL
                     QVKKGLFAEVRVPTAFHEGDHVDLPVTIHNDELEQGQIRLVSRVTIGTRTITESRSLE
                     VTRKGLLTVSLPLKAELPEEVRQTIRSAETPEQLAQALQQNLELEVSLHVGDDRTPVD
                     VVRYNVPLQPRGVMEFTSTSGSASSDTTAWLELAPSPDAPWMMEILIGPTIERSLSDI
                     VLGSSQRFLPPCGEGSSLLDRTTSDLMAAVAVLNYFRSIPASADEIVQMVDTRIRIAV
                     STLVAAQRQDGGWSWSGAKRMRMGASDVEVASHPESSARAFWALHLARQAGYPVPQAT
                     YDNALQYLEKAMTQIRADDMEVRSVLIHALALAQRADFALVNRLHRERQNLSLAAKLY
                     LALTLAVMQRQPMASEVLKLVDLSKAEEAAQTPLPRSVALSLTEAHALHALVLQAISP
                     ESPELRTDVEWLLSHRSGHRWLPDRATGLAALVLGPWFAKTRFIDEKYRLTVFVNDFQ
                     VGQWDFDQTSLSQSVLVPPKFFVGGRERIQFRLEGRGRFTYQCLARRFVNPEQARRGH
                     YGSWLRPAPLEVDGREVPRGISSGNEGVFVKPGWSLPHSRVTQLPVGKQAIFEITAAP
                     RVLTAGPVIDQFVLTVPIPAGTSVVPNTIVGPFDRYELRAQEIVFFFARPRSEAVTVN
                     FRVTGTIPGDYVVEPAVIRNALDPTAYAVIPLSDAKDQTSASLKVLPEGVSSTDPYFL
                     SPLELIALGKSAYAKKDWKSAEKYFAELFTNPNWQVMDETVKSLAPLLVDIYLELDQP
                     DKVVHYFEILKVKWPDYEIPFDKILRIAACYERLGEYERSFLIFRAILESSFVNDSYT
                     AGFLDEQNEFVRSVQLMNRLLAEYPPEPYAAAARYALAQRVYAKANEATADMALRKAK
                     ITRVHLISAAWEMLDRFLTAFPEDPAADQAAFAAAHALLDCEFYSQAAEAAGRYAQRY
                     AESSLLDSFWYLIGYCHYADGRHEQAIEMCRKVADWQRKDPATGRTEESKNKWQALYI
                     LGQIYHSLGQPLQAINYYQLVSDRFSDAKEAIDYFKRKAVALPEVTFIKPGEPVTLTL
                     RSRNITQCELKAYRIDLLKFTLLKRDFSGIVGINLAGIRPEYEETIQLGEAQDFRDHE
                     HQVKLPFDKDGAYLIVCRGDDLYASGLALISPLRMEVQEDAVSGRVRVTIKNVDGNFV
                     NNVHVKVVGSRNAEFVAGRTDLRGVFVADGIQGRVTVIAQKDSNQYAFYRGETDLLPA
                     PARPGAPPAPSERKPQAAGEDRRELLRGLEESNRMLQQQQIENLRQNYQQQRKGVEAS
                     KAF"
     gene            complement(4562..6208)
                     /locus_tag="PLD05_05265"
     CDS             complement(4562..6208)
                     /locus_tag="PLD05_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOP76877.1"
                     /translation="MQWAVCARHEQCFLPVSDQTVLELGHVVLLLGQDLPQGAHAGKT
                     PAENSSLIRKILAIFSRDPLALATVLGSIPPASESGHDEDAPEVALGRAILQGFWACL
                     EDDRHSPPLKLVEPRAAEKFSIDKGRILQTVRKIFLIQQLTRNWAELHQSQAVPLIPF
                     AAVVTVSRLFLGDCSVDFVAELRRVLNPKDHKTLDVALEVVETCHGILGQLGEGAKSL
                     QLDSQLLAPLEGTQACDPGEAKQATDGTACSADAVTGAYASLIGSEEVWRAAEHRACR
                     LARRWGRSVPGCRGLWETVLLVTRRLARLNARFAEALEHAKLDAMAEFAAGAGHEINN
                     PLAIISGHAQVLLRQVTHPEWQRMLAAIVAQAQRAHEMIADARLFARPPKPVFAPVNY
                     RDIVQTVIKEYTPPAESRHVQLRLATAVEAQDRFQGMADRSQILVALGAIVKNALEAV
                     PEKGTITLTLDADTEEVRISIADDGPGIDPKVREHLFDPFFSGRQAGRGLGMGLSKAW
                     RIVTLHSGTIEVQSAKGSGAIFSIRLPRNPPGGGPSSSGP"
     gene            complement(6313..6930)
                     /locus_tag="PLD05_05270"
     CDS             complement(6313..6930)
                     /locus_tag="PLD05_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76878.1"
                     /translation="MAKKRNSQSPLASKTVFTTGEAAKICKVSQQTIIRCFDSGQLKG
                     FRVPGSRFRRIPRDQLIAFMRENGIPIDNLENTKHKILIVDDDQELVELIRDVLERDG
                     RFEVQSVNNGFGAGMMVKEYHPDLIVLDIMLPDINGKDVCKVVRSDPNLSEIRILCIS
                     GMVEEDRIEELKAAGANDFLHKPFEIEDLVERICQLLDIPSRATT"
     gene            complement(7621..8703)
                     /locus_tag="PLD05_05275"
     CDS             complement(7621..8703)
                     /locus_tag="PLD05_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76879.1"
                     /translation="MARQKSSPKRTTQKMTPASRTQQVIRLSELLSKAYRKRPADVER
                     PVLEQLLFAALLENAHYEAALRVFNALKEEFFDWNEVRVSTVRDLVDIATGLPDPTAA
                     AHRIKQPLQDIFESTYSFDLDDLKKVPIGQAVERLQKIDGVTRFMIGYVVQNALGGHT
                     IPLDAATLQVLNILGLADESEVASGVVGGLERAIPKSQGRDFAWALHEFAAEFWADHH
                     SPQVTKIMKAFDPQSLQRLEAWEAQATRQAEPSREAVAESTAVAEVAAATTTGQAEGQ
                     TGRGRRKKGEAETPVEPVVTSQQAVQPAAKEQEAATVSVPPGEKGRRGRPPASGEAAS
                     ERKSRRGRSPKGVSDSEKKRGRKKAT"
     gene            complement(8808..9239)
                     /gene="rbfA"
                     /locus_tag="PLD05_05280"
     CDS             complement(8808..9239)
                     /gene="rbfA"
                     /locus_tag="PLD05_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOP76880.1"
                     /translation="MSSRRALKLAEAVRQVVSTAILFELKDPRVRDVTVTYVEVAEDL
                     RSAKVHVSVMGDEKKQQLTLRGLQNAAGFLQSRCAEKIETRWTPRLQFVLDKGVKHSI
                     EVCRILAEVLPPTPSDQAAAEITEEEQAEEGSENQDESAED"
     gene            complement(9342..12584)
                     /gene="infB"
                     /locus_tag="PLD05_05285"
     CDS             complement(9342..12584)
                     /gene="infB"
                     /locus_tag="PLD05_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOP76881.1"
                     /translation="MTQRIYSLAKELKLESKELMDICRELNLGKASALASLSDEEVHL
                     IKEHIKRARGAGARPATASTVSEPAPVFRREDYIPPAGTKPGKVPVLDRKIERPSPRP
                     EVPPSERPTPVTPTEPSPAVTATVEVSAPPTAGEVPLEPSGQVPVAPEVPPPLASETL
                     ETVVPTTGAPSAEVVEPPVAVEEKGPPRAVEVETPSVGPAVEVAELTAPEAVAAETPV
                     VAPAEVSVEGIARAEEVSPAQATAATAVSEPSTAVPEVPLVTEVSDREITPQTTQEVT
                     SEVTAVAPSSERKEEPPPPGLPPTEAERLAAIRPIPVLPRSRREAASQPGQPAEKKKK
                     TKAEPPLPRLVKLAPLPTPKPAPVPKPKEPAPQKPEIKLPVDAIRASKMGAMPLAEHL
                     LRQQEEKRKGKKKEKEETKGDISVPVLPRQKGRRTKEEELEAGEGPIGKVKRKKEAVR
                     VEDEEEAHEELEATSVQPTRLRRTRSVSTAAPRKSSVVLELPCTVRTFAEALGLPAQK
                     VLAKLFEMGTLANINATLTQEMAELLAVELGVDVVFKAETDPEEELNAIWNAPDPEDS
                     LRPRPPVVTFLGHVDHGKTSLLDKILGINVAAKEKGGITQHIRAYQIVKNGRPITFLD
                     TPGHEAFTAMRARGANCTDIVVLVVAADDGVMPQTEEAINHARAANVPIVVAINKIDL
                     PGTRAERVMQQLTGLGLVPTEWGGDIEVIRTSALTGEGIPELLDTLITLGDLYELKAN
                     YDRPACGTCLEASLQPGRGVVAKLLVQKGTLRPGDIVVCDVAHGRVKAMYDPLQPHKR
                     IQEATPATPVDLIGLDAVPEAGSRFVVLDDIAKARKIAEHRMETVHRREIMEARRHVT
                     LENLLEQLSEKEKKTLHIILRADTRGSIEAIQKEIAKLEHPEVKIQVLQALVGGITVA
                     DVHLADASDAVIIGFNVVPDEDARELAERLKVQIRRYDIIYQVADDLKAALEGLLQPE
                     KREVELGRALIQKVFHISRIGNVAGCRVLSGTITRDARLRVIRQNRIVGDYPIESLKR
                     EKDDVREVREGFECGIKLAGFDDIKEGDILEAYRIEEVKRTLETASQSTT"
     gene            complement(12869..14212)
                     /gene="nusA"
                     /locus_tag="PLD05_05290"
     CDS             complement(12869..14212)
                     /gene="nusA"
                     /locus_tag="PLD05_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOP76882.1"
                     /translation="MNPDELLRIVDAIHREKNIEKEVVFKSIEAALATVARKRYGDQA
                     DISISIDRKSGVISGRYGDQAIDPEQLSRIGAQAAKQVILQRIREAERDVLYEEYQEL
                     LDQVVTGTVQKVEPRAIIVSLPRTEAILPKSEQIPGETRHPNERIRAVVTEVKKAGTR
                     LRIVLSRIRPVFVVRLFEQEVPEIADGVIEIKSIAREPGYRTKVAVASVDQRVDCVGA
                     CVGLRGNRIRNIVEELSGERIDIIRWDDDLMTLIPNALQPAKVDEVILCQMLGRAIVL
                     VPEDQLSLAIGRWGQNVRLASKLCGWDIEIMTRDELDEQLEQAVLGFSSLEGVTPELA
                     DRLVGEGFLSYDDLSVIEPEDLMEMGGLTEEQVQHIIAQAEAKAEEAEQLAREEKRRQ
                     RQLEREREAALAEGVTSEVPPSAESGEAVQDQEEAGPKEDFTQEASSGLGEGTSS"
     gene            14784..15599
                     /locus_tag="PLD05_05295"
     CDS             14784..15599
                     /locus_tag="PLD05_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP76883.1"
                     /translation="MAWPFLRIGNQTAFSAHHPDDPFRFAIEQGFDAFEWFNDKRHGW
                     GWCQEDVDDYDREQIRQLGEKHSIVFSVHARCQANINTPQGRDDVLRSIEFAKDIGAV
                     LVNVYYHGDQGPQGFAASLAPLVEAAAPANIRISVENTVFVSPHELNELFAALDATDW
                     CRPGAVGVCFDMGHANLCAATRYDYVGFLNQLESNVPIIHLHVHENWGDGDSHLPLFT
                     GPAAQDDSGVRTMLGILRERGYDGLIIMEQWPQPADQLITTRNRLRELLHAVS"
     gene            15840..16949
                     /locus_tag="PLD05_05300"
     CDS             15840..16949
                     /locus_tag="PLD05_05300"
                     /inference="COORDINATES: protein motif:HMM:NF017003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HOP76884.1"
                     /translation="MNARMWLGSVAVCLLTASAALAQYGPYYGAGTAPAWQGVMPTGY
                     FEGNNGQALAPTPDQGTATTSLGPCPTGCLPTWQVWGEYLYLRPSDADVPWAVAINGA
                     IQQGSVPIQVSRVANADIDFDSGFRFGVGRALDECASVGVSYTFFESHSNDSLATDDP
                     YVLRSLVAHPGTLTAAADYLRGIASNDVDFQLIDADYRHVFRSNCYGSMNWLVGARYA
                     QFEQDFNALFRNNGIETVLTESRFYGAGLRLGLEGERHSQKCGLLIYGRSTANFVAGD
                     FRARYQQSQSYDVIEVDTRLRASRVVPILDLELGSGWVSQDGRCMFTVGYMVSAWFNV
                     LDADTFIRGVQANDFSDLDDTVVFDGLVGRAEIRF"
     gene            17413..19551
                     /locus_tag="PLD05_05305"
     CDS             17413..19551
                     /locus_tag="PLD05_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP76885.1"
                     /translation="MSHRRAFPCAIICGLVLGVMSIKSSSLAQDFYVSPEGNDRWSGR
                     LATPNAEKTDGPFASLARAQQAVRELRNQQPDRDRPIVVAIRGGRYFLSEPLRFGPED
                     SGTEKSPTIYRAHENERPIISGGVSLRGWTVDSQGRWHLMLDDVKAGKWNFTQLFVND
                     QRRYRPRLPKKGYYTIAERVPPPADNPVKGDDRLQYAEAEFNPQWSNLGDVEVNIFHQ
                     WSMSRMRIATIDASKKLVVFRRGTGFDSHWAHFLKGNRYYIENVADALTEPGEFYLDQ
                     KKGELIYIPRPGEDPQRADVIAPRLENLVIFACDGANKRWVEHLQFHGLIFAHSNWVC
                     PENGQFYPQAEVGLDGTISAIGARNVVFDACAVIHTGTYAMAFGEGCRNCRVENCELV
                     DLGGGGVKIGHAGSANWQGASQRPQGEEALVSHITVKNCTIAHGGRIHPAAVGVWIGH
                     SAYNTVEHNDIFDFYYTGVSVGWTWGYADSPAHHNAINYNHIYNIGQYVLSDMGGVYT
                     LGVSPGTTVNHNKIHDVYSFSYGGWGLYTDEGSTHIEMAYNLVYRTKTGSFHQHYGKE
                     NRIHNNILVNSVEHQIQRTRTEEHISFFFERNIVAWDNDSPLLGSNWKDNNFRMDYNL
                     YWHGGKPVVFPGNLTLEQWQKERGQDVHSIIADPKFVDPAKDDYRLQPDSPAFSLGFK
                     AFDPNEAGRTSPPQLTANLPEVPTGLAKGE"
     gene            19594..21354
                     /locus_tag="PLD05_05310"
     CDS             19594..21354
                     /locus_tag="PLD05_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOP76886.1"
                     /translation="MTTVRCIIRALWVTSCLAWLVLCQGAVKGEESTSIWSAPPESLA
                     KWQAMRFGLFIHWGPVSLKGTEIGWSRGAQVPIEEYDNLYKQFNPTKFNADEWVSIAK
                     EAGMKYLVITSKHHDGFCIWDSEYTDYDIMSTPFKRDILKELSEACRRQGIVFCAYHS
                     ICDWYHPDYPLGSPGGRTKKPNPNMERYVQYLHNQIAEIIRKYGPLGVIWFDGDWEEP
                     WTHEHGLALYKYIRTLQPDILINNRIDKGRRGMKGDTAGPQFVGDFDTPEQRVGAFNR
                     ERPWETCMTICRQWAWKPNDELKTLSQCLEILLRTVGGDGNLLLNVGPMPDGQIEPRQ
                     VERLKEIGAWLKKYGDGVYGTRGGPFKPGPWGASTCKENKIYVFIFNWPKEGPLVLPA
                     INQKIVSAVARTGGDVKVEQSNTQITVSLSPEQRDPLVTVLELTVSGTAFDIPPVAVP
                     ALSGALSVNRPTKASNVFQKQVARFGPQMAVDDDSETRWATDAGTHSAWLEVDLERPC
                     VVDRAVIEEAFAPRVQEFQIECAQGDEWVPCFKGTTIGERGEFRFRAVTGQRFRLNIL
                     KATEGPTIWEFQLFGSEKKE"
     gene            21374..22198
                     /locus_tag="PLD05_05315"
     CDS             21374..22198
                     /locus_tag="PLD05_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOP76887.1"
                     /translation="MSRSWIRVDSLDDPRLAPYRNLPERTLRGESIFITEGWLVTERL
                     LKSTYETESVLLAEDCAAEYADQVPDSIPLYVGPRELLYQVVGYQFHQGVLAVGRRRI
                     LPSVRDVLPPPDRPGPLMVVVCPQITKPENIGLVFRSAAAFDVDAVILGERCAEPFSR
                     RILRVSMGAVLHLPMAKTNNLREELAILKREWNVALVASVLDPTAVSLPDFSPPERVG
                     LLLGHETEGLREGWADLCDYSVTIPMSSLVDSLNLGVAAGIFLYEFRRNGLKRRSA"
     gene            22342..23772
                     /locus_tag="PLD05_05320"
     CDS             22342..23772
                     /locus_tag="PLD05_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOP76888.1"
                     /translation="MGSGAILNDWDSLWENDIRFPQCHSHEQLARLFPEINVSFVASF
                     NENHAPLAFWCDDHWPEQPAITLKNLRSFLPETMTPTEWRPFAYGEGQNILGFVTRFQ
                     APEECYQMKSPPAGFVVGLYRGTEQAFCAAAPLLIPFARALGLSAWLADRLLLSCTKI
                     EQMEQEKISLKQSHSEAVSMALEEHELVLLAEEKQKVLQELIEAKEAAYRAKSQFLAN
                     ISHELRTPLHAILSYAAFGIKKIETADKPTLLRYFQQIETSGKSLLAFFNDLLDLSKL
                     EAGKMRYERSVSEVVWLIHSVLEEFRSLAEQKRIKLEYIGPQCFRTELDPTRFAQVIR
                     NLISNAVKFTREDGHVWVTLVPQEDHFLLRVDDEGCGIPDGELDSIFESFYQSSRTRT
                     SAGGTGLGLAICREIVLAHGGRIWAENRPEGGARFSVFWPITRTTDHLDSQGNPHAPP
                     ATVVGADDEASSSNTQDVLVTATAEA"
     gene            23956..24948
                     /locus_tag="PLD05_05325"
     CDS             23956..24948
                     /locus_tag="PLD05_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP76889.1"
                     /translation="MKTRIILCRFNQIVVSIPSLMLLLVAMGVGRDGIASESQPGQQV
                     SKKIVFIAGRPSHGFGSHEHNAGCLLLAKKLKEALPNVETVVYTNGWPQQTDALTDAD
                     AIVIFADGGGGNIIAQHLDELQKAIDKGAGLAVLHYALDIAAPEGREKLKSWIGGYYE
                     TYWSVNPHWRAEFKALPEHPITRGVRPFAIDDEWYYHMRFVDDTTRLVPILTAIPPDS
                     TRERPDGPHSGNPTVRSRKGMPEHVAWAYERPDGGRGFGFTGGHHHWNWAHDQFRTLV
                     LNAIAWCARIEIPEQGLRTTTPTIEELLANQDEPPPANFQPETIKRQIESWNQQ"
     gene            25041..26966
                     /locus_tag="PLD05_05330"
     CDS             25041..26966
                     /locus_tag="PLD05_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP76890.1"
                     /translation="MTCQPVPFFRSSRAILWPALLVTLSLALGRAAFGEEKAPEAAPP
                     QGLRILFLGDNPWNGGTLRRVFEAFAATYRRNIEITEIPAKDRPLGEVIAGLIESTPE
                     KGRFDVVVAFDHPLRQVVQRDRAELITEIDTLEGLAPRIVWILSPYRKPTLRVETERQ
                     KILGRLPLPQGFSTLPLIRLFHQTRKLAGGAAWVNDLSLDRPTSFSDYLTAALLAQVL
                     WGPAPQFPNDIRISDTASLRIPEEALPVLAKVLATLEITLPKGPALANAFAPELSPPQ
                     RFVGNGLPLTLTSGVIPGFFDWNADGRLELVLCGRETGQWEIWTWPTPDKPLSLAQAA
                     RFPAETVNPDLAAMIKAGATVFQVVDFDYDRHPDLLIGTNEGKLALCRGQAGMVFGRP
                     EVCMLEDGKPFQVPYLHRAFLGELTKDGVWDLVLGDGAGQVFLTSLVEQGGRFLWGQP
                     RPVTIEGLTLQVDGEASPVIADWDLDGLPDLIVGCRNGGVLWIRNVGSNEQPAWAPPV
                     SLVWPPEEEETLLIPYRNGMELWTPTPGWSTQPSVADLDGDHLPDLIIGDSNCRVVKY
                     RDFTAEEQKEVDALLKKRREIVEKLSQSPPEEIARQKAALWEITLELIQKSTDRRYER
                     CGWVWYLRRQSLATPDE"
     gene            27226..>27740
                     /locus_tag="PLD05_05335"
     CDS             27226..>27740
                     /locus_tag="PLD05_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76891.1"
                     /translation="MAEHGNRRQFLNYGLLGAMGAAAASSLEERILLAALQEGAPEKA
                     AQTPVDDRPMPCGRIRNLTISRMLIGGNLIGGWAHSRDLLYVSRLFKAYNTEERIFQT
                     LALCESVGINTVQLDPACINVFHRYLKERGGKMQALVCIHPDADAGKVRSQIEDLLNK
                     GATFLYTHGEVT"
BASE COUNT         5824 a         8068 c         7727 g         6121 t
ORIGIN      
        1 gccgctcccg caccagcaca acctccgcaa tctgccgcgg aaaagcaaca ggatgagaag
       61 gatatggcag atcgacttgc cgcagatgga cgcgagcggc ggagttttaa ggcccagagc
      121 ggcggcaagg ccgaagacaa ggataaagaa ttgctgggga aattgactgc gaatcgcctc
      181 cgggtatggg actcccggca agactttttg acgcccagcg tagaaaacct ccagcgcgtc
      241 ggcgggacaa cacgtcaaaa tttccttgtc ctcgataaca aggggcgatt gcaatacttc
      301 ggccaaaacc aactcgctca agcggacgta agggccctgg ccagagagtt ccaaaaacgt
      361 ggcatcgtcc tttatcccga gtggacaccc cacgaaacgg ctttctggga cccgatggtg
      421 gaaaccaacg acagtggcaa ggccgtggtc aaggtcgtcg ttcctgaccg tgccacctca
      481 tggaagctgt tggccaaagg tgttaccgtc gatacccggg tgggtgagtc cgaggcggaa
      541 cttcaagtca agaaaggcct ctttgccgaa gtcagagtcc ccaccgcctt ccacgaaggc
      601 gaccacgtcg atcttccggt gaccatccat aatgatgagc ttgagcaagg tcagatcagg
      661 ctcgtcagtc gggtgacaat cggtactcga acgatcacgg agagccgatc gctcgaggta
      721 acccgcaaag gcctcctgac agtaagccta ccccttaagg cggaacttcc cgaggaggtg
      781 cggcaaacca tccgcagtgc cgagaccccc gagcaattgg cccaggccct tcagcaaaat
      841 ctcgagttag aagtcagtct ccacgtcggc gacgacagaa cgccggtcga tgttgtccgc
      901 tacaatgttc cgcttcaacc ccgcggggtg atggaattta cctccacaag cgggtcggcc
      961 agcagcgata ccacggcctg gctcgaactg gctccctctc ccgacgcacc gtggatgatg
     1021 gagattttga tcggtcccac gatcgagcga agtcttagtg acattgtctt gggaagtagt
     1081 cagcgtttcc taccaccctg cggggaaggc agctccttgc tggaccgaac gacatctgac
     1141 ctcatggcag ccgtcgccgt gctgaattac ttccgctcga tccccgcatc agccgacgag
     1201 atcgtgcaaa tggtggatac gcgaattcgc atcgctgtga gtacgcttgt cgctgcccag
     1261 cgtcaggatg gcggttggtc ctggagcggg gcgaagcgga tgcggatggg ggcgagcgat
     1321 gtcgaggtcg cgtctcatcc cgagtcatcg gcgcgggcat tttgggccct ccacctggca
     1381 agacaggcag gttaccccgt tccccaagcc acctatgata atgcgcttca atacctcgag
     1441 aaggctatga cgcagattcg ggcagacgac atggaagttc gttccgtgct cattcacgcc
     1501 ctggccttgg cccagcgggc cgatttcgcc ctcgtcaatc gtctccatcg ggagcgtcaa
     1561 aacctctccc tcgccgccaa actctacctt gcgctgacac tggccgtcat gcaacggcaa
     1621 ccgatggcct cagaagtcct caagctcgtc gacctctcca aagctgaaga agcagcgcaa
     1681 acaccgctac cccggtccgt agccttgtcg ctgaccgaag cgcatgccct ccacgcgttg
     1741 gtcctccagg ccatttcgcc tgaatcaccg gaattgcgaa ccgacgtgga gtggctgctc
     1801 tcccatcgat caggccaccg ctggctgccg gatcgagcaa cgggtctggc ggcactcgtc
     1861 ctgggcccct ggtttgccaa gactcgtttt atcgacgaga agtatcgcct aaccgtgttc
     1921 gtcaacgact tccaagtggg acaatgggat tttgatcaaa cgtccctctc gcaatcggtc
     1981 ctcgttccgc cgaaattctt tgtcggagga cgcgaacgca ttcagttccg cttagaaggc
     2041 cgtgggcgat ttacctatca gtgtcttgct cgacggttcg tcaatcccga gcaggctcgc
     2101 cggggacact acggatcatg gctgcggcct gcccccttgg aagtcgatgg ccgcgaagtt
     2161 ccccggggca taagtagcgg caacgagggg gttttcgtca agccgggctg gtcgttaccc
     2221 cacagccgtg taacgcaact cccggtgggc aaacaggcca tcttcgagat cacggccgcc
     2281 ccccgcgtac tgaccgcggg cccagtcatt gatcaattcg tgctaacggt gcccattccc
     2341 gccggcacat cggtcgtacc caatacgatt gttggcccgt tcgatcggta cgagcttcgg
     2401 gcgcaagaaa tcgtattctt ctttgcccgg ccaaggtccg aggccgtgac ggtcaacttt
     2461 cgcgtgacgg ggaccattcc gggagattat gttgtggagc cggctgtcat ccgcaatgcc
     2521 ctggacccca cggcctacgc ggtcattcct ttgtccgacg ccaaagacca gacgtcggct
     2581 tcgttgaagg tcctccctga gggtgtttcc agcacggacc cgtacttcct ctcgccgttg
     2641 gaactgattg ccctcggaaa atccgcttac gcgaagaagg attggaaatc agcggaaaag
     2701 tatttcgcgg agctgtttac caatcccaac tggcaggtca tggatgaaac ggtcaagtcg
     2761 ctcgctccgc ttttggttga tatctacctg gaattggatc agcccgacaa ggtggtccac
     2821 tatttcgaga ttctcaaggt caaatggccg gattacgaaa tccccttcga caagattctc
     2881 cgtatcgccg cgtgttatga gcgattggga gaatacgagc ggagcttcct gatctttcgg
     2941 gctatcttgg agagtagctt cgttaacgac agctacactg ccggctttct cgacgaacaa
     3001 aatgaattcg ttcgcagcgt gcaactcatg aatcggcttt tagcggagta tcccccggaa
     3061 ccctacgcgg cggcagcccg ttacgccctg gcgcaacgcg tctatgccaa agcgaacgaa
     3121 gccaccgcgg atatggctct gcgcaaggcc aagatcacgc gggtgcatct tatttccgcc
     3181 gcctgggaga tgctcgatcg gttcctgacg gcctttcccg aggatcccgc ggcagaccag
     3241 gccgccttcg ccgccgccca cgccttgctg gattgtgaat tctacagcca ggccgccgag
     3301 gcagccgggc gttatgccca acggtatgcc gagagctcgc tcctcgacag tttttggtac
     3361 ctgatcggtt actgtcatta cgccgatggt cggcacgaac aggccatcga gatgtgtcgt
     3421 aaggtcgccg actggcaaag gaaagaccca gcaactggcc gcactgagga aagcaaaaac
     3481 aaatggcaag ccctctacat ccttggccag atttaccaca gtctaggtca accgttgcag
     3541 gcaatcaatt attaccagtt ggtctccgat cgcttctccg acgccaagga ggcgattgat
     3601 tacttcaaac gcaaggcagt cgcgctgccg gaagtcacat tcattaaacc cggtgagccc
     3661 gttacgctga cgctgcgatc gcgtaacatc acgcagtgcg aattgaaggc ctatcgcatc
     3721 gacttgctaa agttcaccct cttgaaacgc gacttttcgg gaatcgtggg aattaacctg
     3781 gccgggatcc gccccgaata cgaggaaacg atccaattgg gcgaagctca ggactttcgc
     3841 gaccacgaac atcaagtcaa gctgccgttc gacaaggatg gtgcctactt gattgtgtgt
     3901 cggggcgatg acctttacgc cagcggattg gctctcattt cgccgctgag gatggaggtt
     3961 caggaagatg cggtctccgg ccgtgttcgc gtgacaatca agaatgtgga cggcaacttt
     4021 gtcaataacg tccacgtcaa ggttgtcggc agccgcaatg cagaattcgt ggcaggccga
     4081 accgatttgc gcggtgtctt cgtggcggac ggaattcaag gcagagttac ggtgatcgcc
     4141 caaaaggaca gcaatcagta tgccttctat cggggagaaa cggacctcct cccggcgccg
     4201 gcccgtcccg gagcaccccc ggctccgagc gaacgcaaac ctcaggccgc cggggaagat
     4261 cgccgcgaat tgctgcgggg actggaagaa agtaacagaa tgttgcaaca gcagcagatc
     4321 gaaaacctgc gtcaaaacta ccagcaacag cggaaaggcg tagaagcctc caaggcattt
     4381 tgaacaatca cgacacttta gcctcgcctc cggatactag gtatccactc cagtaaatgg
     4441 ctgggatgca aagataccct taagccccac ccgataggat ccgcgcatta tcgccattct
     4501 gccggacagg cgcggcaatc ggcggtttta agagtttcgt caaattctcg ccacatccct
     4561 cttacgggcc agaagatgat ggcccacctc ccggaggatt ccgcggcagt cggatggaaa
     4621 agatcgctcc acttcccttg gccgattgaa cctcgatcgt tccactgtgg agcgttacga
     4681 ttcgccacgc cttcgacaat cccataccta aaccgcggcc ggcttgtcgt ccggaaaaaa
     4741 acggatcaaa aaggtgttcg cggactttag ggtcaatccc cggcccatca tctgcgattg
     4801 agatccgcac ctcttctgtg tcggcatcca gggtcagtgt gatcgtcccc ttctctggga
     4861 cggcctcgag ggcattcttc acgatcgccc cgagggcgac gagaatctgc gaccggtcgg
     4921 ccatcccttg aaaacggtcc tgtgcctcga cggccgtcgc cagccttagc tgcacgtgcc
     4981 gcgattccgc cgggggagtg tattccttta ttaccgtttg cacgatgtcc cgataattca
     5041 ctggtgcaaa cacgggcttc ggtggccgcg caaagaggcg ggcatcggca atcatttcat
     5101 gggcacgctg ggcctgcgcg acaatcgcgg cgagcatccg ttgccattcg ggatgagtca
     5161 cctggcgcaa aagaacttga gcgtgtcccg agatgatggc cagcggattg ttgatttcat
     5221 gtccggcacc ggccgcgaat tccgccatgg catcaagctt ggcgtgctcc agggcctccg
     5281 caaaacgggc gttgagcctg gctagccgtc gcgtcacaag taggactgtc tcccaaagcc
     5341 ctcgacaacc ggggacggat cgcccccatc ggcgtgccag ccgacacgca cggtgttcag
     5401 ccgcccgcca tacctcttca gatcctataa ggctcgcata agcaccggtc acagcatcgg
     5461 cagaacaagc cgtgccatcc gtcgcctgct tggcttcacc aggatcacag gcttgcgttc
     5521 cttcgagcgg ggcgagcagt tgcgaatcca attgtagcga cttcgcaccc tcgccaagct
     5581 gcccaagtat cccgtgacag gtctccacaa cctcaagggc aacgtcgagg gtcttgtgat
     5641 cctttgggtt caggactctc cgcagttccg ccacgaaatc cacagagcag tcccccaaga
     5701 aaagcctcga gacggtgacc accgctgcaa agggaatgag cggcacggct tgggactggt
     5761 ggagttccgc ccagttccgg gtcagctgct gaatgaggaa gattttccga acggtttgaa
     5821 gaatccggcc cttgtcaatt gaaaatttct ctgctgctcg cggctcgacc aacttcaggg
     5881 gcggggagtg cctgtcgtcc tcaagacagg cccaaaaccc ctgcaaaatc gctcggccta
     5941 gggccacctc gggcgcgtcc tcatcatggc ccgactcgct ggctggtgga attgacccaa
     6001 gcaccgtggc aagggccaac ggatcccgcg agaagatggc cagaatcttc cggatcaacg
     6061 aggagttctc cgcgggtgtt ttaccggcgt gcgcaccttg agggaggtcc tgcccgagga
     6121 gcaagacaac gtgccccaac tccaacactg tttggtcaga gacggggagg aagcattgct
     6181 catgtcgcgc acagacggcc cactgcattg caagcccctc ctgctggaac tgcctctatc
     6241 accggtcaaa ggcagaccac tcccgcggat tcttgtttcc tgcaagcgag ccgtctcgca
     6301 ctggtgaatg aatcaggtcg tcgctcggga cggaatatcc aggagctgac aaatgcgttc
     6361 cacgaggtct tcgatttcaa acggcttatg gaggaaatcg ttggcccccg ccgccttcag
     6421 ttcctcgatg cggtcttctt ccaccatccc ggagatgcac aaaatccgga tttcgctcag
     6481 attgggatcc gagcggacga ctttgcagac gtcctttccg ttgatatccg gcagcatgat
     6541 atcaaggaca atcaggtcgg gatggtattc cttcaccatc ataccggcgc caaatccgtt
     6601 attgacgctc tgcacttcaa accgaccatc ccgctccagg acgtcccgga tcagctcgac
     6661 gagttcttga tcgtcgtcca cgattagaat cttgtgtttc gtgttttcca ggttgtcgat
     6721 ggggatacca ttttctcgca tgaaagcgat caattgatcc cgggggatac gacgaaatcg
     6781 gcttcccgga acgcgaaatc ccttcagttg gcccgagtca aagcagcgaa taatcgtctg
     6841 ctgactgacc ttacagattt tggccgcttc accagttgtg aagacggtct tacttgcgag
     6901 tggtgactga ctgttccttt tcttggccac ggttctcgcc ggaatcttcc aaaggagacc
     6961 ccagcccatc ggtcggcagt tggctgagtg tctctggtcc tccgtgttac tccatccggg
     7021 aaagaagccg caggttcacg gtttcccgca aaccttgcac ctcccgtagc tgcctacaag
     7081 cagcaggctc atccggttct gccgacttta ccgaatttgc caaccgcgga tcattatact
     7141 ttcaccaaaa tccctgtcaa tacgactttt gcatctttaa ctctcttgcc cacaccgtgt
     7201 tacgtcccca agacttcccc atgcgaactt tttaccgaat cacagatgaa aagccgcgca
     7261 atttgcggta attggcgttg atggaaagcg gctagccccg acctctcgtg gcggtaccgg
     7321 cagctagccc cgcgaatgcg cggaggggtc agcttttctc ggctgagcca agtgggtcta
     7381 atcagtcccg gcccggaacc gtggggaatc tagccttcga cttttaccta tctcatgatc
     7441 tcccccagaa atacccacct tatgagacag tcgccgaatc tttccgatgg ggcggcaaca
     7501 tcacggccct catggcgaat cggccgccac gagacgacct attaccccta ttcttcccca
     7561 ttttgggcga tgcctgccta gggcgcggaa ctcatataac agagacaagc gcttttttga
     7621 ttatgttgcc tttttacggc cccgtttttt ctcggagtcg gatacccctt tcggcgaacg
     7681 cccgcgccgg gatttccgct ccgatgccgc ctcaccggag gcgggcgggc gaccccgtcg
     7741 ccctttttcc ccaggtggaa cagagacggt agcggcttcc tgctcttttg ctgcgggctg
     7801 cacggcttgt tgagacgtga ccaccggttc gaccggggtc tcggcctcgc ctttctttcg
     7861 ccggcctcgc ccagtttgac cttcggcctg tcccgtcgtt gttgcggcgg ccacttccgc
     7921 aaccgccgtc gattcggcaa cagcctcgcg ggagggctct gcttgacggg tcgcttgggc
     7981 ttcccaggcc tccagccgtt gcagcgattg cggatcaaac gccttcatga tcttagtaac
     8041 ctggggggaa tgatggtccg cccaaaactc cgcggcaaac tcgtgaaggg cccatgcaaa
     8101 atcccgccct tggctcttgg gaattgcccg ttccaaacca ccgaccacgc ccgaggccac
     8161 ctcagactcg tcggccaaac caaggatatt caggacctgc aaagtagccg catcgagtgg
     8221 aatggtatgc cccccgagcg cattctggac gacgtagccg atcataaagc gggtgacgcc
     8281 atcaatcttc tgaagccgtt ccacggcctg tccgatcggt acttttttca gatcatccag
     8341 gtcaaaagaa tatgtggact caaagatatc ctgcaggggc tgcttgatcc ggtgggcagc
     8401 cgcagtagga tcgggcaacc cggtggcgat gtcaactaag tcccgaaccg tgcttacccg
     8461 tacctcgttc caatcaaaga actcctcctt cagggcattg aacacccgaa gggcagcctc
     8521 atagtgggcg ttttctagca gtgctgcgaa gagaagctgt tcaaggaccg ggcgttccac
     8581 gtcggccggt ctttttcggt aggctttgct taaaagttcg gagagacgta tcacctgttg
     8641 cgtgcgactc gcgggtgtca ttttctgcgt ggtccttttc ggcgaggatt tttgccgggc
     8701 catgggaaat ctcccgatca aaggtcccct cgtccttatt ccaaaaacgt gttaagcgtt
     8761 tttaatggct ggtcaatcgg tgacccatgg ttttgttgcg gggtgagcta atcctctgct
     8821 gattcatcct ggttttcgct gccctcctct gcttgttctt cctcggtgat ctccgcggcg
     8881 gcctggtcac tcggcgtggg cggcaagacc tcggcaagga tgcgacagac ttcaatggaa
     8941 tgtttcaccc ccttgtcgag gacgaactgg agacgcgggg tccagcgtgt ctcgatcttt
     9001 tcggcacaac gactttgcaa gaatcctgct gcattctgca agccgcggag ggtcaactgc
     9061 tgttttttct cgtctcccat gacggaaaca tgaaccttgg ctgagcgcag atcctcggcg
     9121 acctccacgt aggtgaccgt cacatcccgc acgcgtgggt cttttagctc aaagaggatc
     9181 gccgtactaa caacttggcg cacggcttct gccagtttca gggcgcgtcg cgaactcatg
     9241 acgacctcct cttggaaaat acctggcaca gaagcgggcg agtcatgggt accacatgaa
     9301 agaaggacat cccttcccac agatcatctc gaccttcgaa ctcaggtggt cgattggcta
     9361 gccgtttcca gggtccgttt aacctcttca atccgataag cctccaggat atcgccctcc
     9421 ttaatgtcgt cgaaaccggc cagtttgatg ccacattcaa atccttctcg cacctcccgg
     9481 acgtcgtcct tctctcgttt caaggactcg atcgggtaat cgccaacgat tcggttctga
     9541 cggatcacgc ggagtcgggc gtcgcgcgtg atggttccgg aaagtacccg acatccggcc
     9601 acattgccga tacggctaat atgaaacacc ttttgaatca aggcccgacc tagttcaacc
     9661 tctcgttttt cgggctggag caggccttcc aaggccgcct tcaaatcgtc cgcaacttgg
     9721 tagatgatgt cataccggcg gatttgcacc ttcaggcgtt ccgctaactc gcgggcatcc
     9781 tcatcgggga ctacattaaa gccaatgatc acggcatcgg aggcatccgc cagatgcacg
     9841 tcagcgactg tgatcccacc cactaaggcc tgcaaaactt ggatctttac ctcgggatgc
     9901 tcaagcttgg cgatctcttt ctggatggct tcgatcgaac cccgggtatc ggctcggaga
     9961 atgatgtgca gcgtcttttt ctctttttcg ctcaactgtt ccaaaaggtt ctcgagcgta
    10021 acgtgcctcc gggcctccat gatttcacgt cggtgaaccg tttccatgcg atgctcggcg
    10081 attttccggg ctttggcgat atcatccagg accacgaagc gactgcccgc ctcagggacg
    10141 gcatccaaac cgatcagatc gaccggtgtg gccggggttg cctcctggat tcgtttgtgc
    10201 ggttgcaggg gatcatacat cgccttaacc cgaccatgag cgacatcgca aacgacgatg
    10261 tcacctggcc ggagggtccc cttttggacc aggagtttcg ccaccacgcc gcgacccggc
    10321 tggagcgaag cctctagaca cgtcccgcac gcggggcgat cgtagttggc ctttagttca
    10381 tacagatccc ccaacgtgat gagggtatcg agcaactcgg ggatgccttc accggtcaag
    10441 gcacttgtcc ggattacctc gatgtcgcct ccccactccg tgggtactag acctaggccg
    10501 gtcaactgct gcatgacgcg ctcggccctc gtccccggca gatcgatctt attgatggcc
    10561 accacgatcg gcacattggc agcacgcgcg tgattgatgg cttcctcggt ctgcggcatg
    10621 acaccgtcgt cggctgctac gaccaagacg acgatgtcgg tgcagttggc cccgcgtgcc
    10681 cgcatggccg tgaacgcctc gtgtcctggg gtatcaagaa aggtaatggg ccgaccattt
    10741 ttgacaattt gataagcccg aatgtgttgt gtgatgccgc ccttttcctt ggccgccacg
    10801 ttaatcccga gaatcttgtc cagcagcgag gtcttaccgt ggtccacatg gccgaggaat
    10861 gtcacaaccg gcggtcgcgg acggaggcta tcttcaggat caggggcatt ccagatggcg
    10921 ttgagctctt cctccggatc ggtttccgcc ttgaagacga catcaacccc caattccacg
    10981 gcaagaagtt ccgccatttc ctgggttaag gtggcgttaa tgttggcgag cgtccccatt
    11041 tcaaagagct tagcaagcac cttttgagcg ggtaggccca gtgcctcggc gaatgttcga
    11101 acggtacaag ggagctctaa cacgacgcta ctttttcggg gggctgctgt ggaaacacta
    11161 cgcgtccgcc gcaatcttgt gggctggacc gaggtggctt ccagttcttc gtgggcctct
    11221 tcctcgtcct ccacgcggac ggcttctttc ttccgtttta ccttgccgat gggcccttct
    11281 ccagcctcaa gttcttcttc tttggtccgc cgtccctttt ggcggggcaa gaccggcacg
    11341 gagatatcgc ccttggtttc ctctttttcc ttcttcttgc cctttcgctt ctcttcctgc
    11401 tgccgcaaca agtgctctgc caggggcatg gcccccatct tggatgcccg aatggcatcg
    11461 acgggcagct tgatctcggg cttttgtggg gccggttcct tgggcttggg cacaggggcc
    11521 ggcttgggtg tgggaagtgg tgcaagttta accaatcggg gaagcggtgg ctcggccttg
    11581 gtctttttct tcttctctgc tggttgacca ggctgggatg cggcttctcg ccgtgatcgc
    11641 ggaagaacgg gaatgggacg gatggccgct aaacgctcag cctctgtcgg cggcaaaccc
    11701 ggtggcggtg gctcttcttt ccgctccgaa gagggcgcaa cagcggtcac ctctgaggtc
    11761 acttcctggg tagtctgagg ggtgatttcc cggtccgaca cctccgtaac caacggtacc
    11821 tcgggcacgg cggtcgacgg ttcacttact gcggttgccg ccgtcgcctg ggctggagag
    11881 acctcctccg cacgcgcaat tccctcgacc gaaacttcag cgggggcaac aaccggagtc
    11941 tccgcagcga ccgcttccgg cgctgtcagt tcggccactt caacggctgg accaacgctc
    12001 ggggtttcca cctcaacagc gcgaggcggg cccttctcct ccactgccac gggcggttcc
    12061 acaacctctg cgctcggcgc accggtggta ggtaccacgg tctcgagagt ctcgctcgcc
    12121 aacgggggtg gaacctccgg ggcaaccggc acttgaccgg acggctcgag aggtacttca
    12181 cctgcggtgg gaggcgcgga gacctctact gttgctgtaa cagcgggcga gggttcggtt
    12241 ggggtaacgg gggtaggccg ctcggagggt ggcacctccg gccgcggcga tgggcgttca
    12301 atcttgcggt cgagaaccgg cacctttccc ggctttgtcc ctgccggtgg gatatagtcc
    12361 tctcgcctaa aaacgggagc gggttcactg acggtcgaag cagtggccgg gcgagcacct
    12421 gccccgcgag cccgtttgat gtgctccttg atgagatgga cttcttcatc cgagaggctg
    12481 gccaatgccg aagctttgcc gagattcaac tctcggcaaa tgtccatcaa ctctttgctt
    12541 tcaagcttca gttctttagc taagctgtaa atacgttgcg tcacgtactc ccctccggat
    12601 ttcaccgatc agcatcggga aaccctctcc catacattaa ctaaccatcg gcagcctcct
    12661 tcgcctagcc ggagacaagc ccatccgctt gtcccgaccg gtgagaaaac ccgacagaac
    12721 taccaactat cacggtctcc gacctgctgc ccctcccatg accccgttgc cgcatggggc
    12781 acccctccca tgaggataac accccagaga ggtaaaagct acgtgcgagc atcccccgtg
    12841 atgccctgtg ttaaagctta accaagcctc aggacgaggt tccttccccc aaaccggaag
    12901 acgcttcctg ggtgaaatct tccttcggcc cggcctcttc ctggtcctga accgcttcac
    12961 cggactccgc tgacgggggc acttcacttg taacaccttc cgccagcgcg gcctcacgct
    13021 cccgctccaa ttgtcgctgc cgtcgcttct cttccctggc caattgttcg gcttcctcag
    13081 ccttcgcttc tgcctgggcg atgatgtgtt gaacctgctc ctcggtcaaa ccacccatct
    13141 ccatcaggtc ttcaggctca atcaccgaca gatcatcgta ggacagaaaa ccctcgccga
    13201 ctaatcgatc ggccagctcg ggggtaacac cctccaagga ggaaaaccca aggacggcct
    13261 gttccaattg ctcgtccagt tcatcgcggg tcatgatttc aatatcccac ccgcacaatt
    13321 tgctcgccag tcgcacattc tgtccccacc ggccaatggc caaggaaagt tgatcttccg
    13381 gcacaaggac aatcgcccgg cccaacatct ggcagaggat cacctcatcc accttggccg
    13441 gctgcaaggc attagggatg agggtcatca aatcatcatc ccagcggatg atatcgatcc
    13501 gttcgcccga cagttcctcg acgatattgc ggatgcgatt acctcgcaaa ccgacgcacg
    13561 ccccgacgca atccacccgt tgatccacgc tggccacggc gactttcgtc cggtaacctg
    13621 gctctcgggc aatggattta atttcgataa cgccgtcggc gatttcagga acctcttgct
    13681 caaaaagccg gaccacaaaa acagggcgaa tgcggctcaa aacaatccgc aaccgcgtac
    13741 cggctttctt gacctcggtc accaccgcgc ggattcgctc gttgggatgg cgtgtttcgc
    13801 ccgggatttg ttcactcttc gggaggatgg cctccgtccg gggcaaactg acaatgattg
    13861 cccggggttc caccttctga accgtccccg tgacgacctg atccagtaac tcttggtatt
    13921 cttcgtagag gacgtcccgc tcagcctccc ggattctttg aaggatgacc tgtttggcgg
    13981 cctgggctcc gatccgcgag agttgctctg gatcgatcgc ctgatcccca tatcgcccgc
    14041 tgataacccc acttttccta tcaatcgaga ttgagatatc ggcctgatcg ccgtaacgct
    14101 tgcgcgcaac ggtagctaag gccgcctcaa tgctcttaaa gacgacctct ttttcgatgt
    14161 ttttttcccg gtggatcgca tccacgatcc gcaatagttc gtcggggttc attgatgacc
    14221 tgtttgtgac tgaaaggttg aaactcctgg ccggtcctgc cgccggaccc ttacactaac
    14281 aaaaaaaccg cccgttgccg tcgtgcagca agtcgccgca caaccggctc gcgacggtta
    14341 atcaaacgaa accttcagcc tttgccgttc cgcgtcctgg cgcccttcgt gcaaaacggc
    14401 catcaacctt gcaaagctgg cggcaactta cctgcctcgg cagtcatttc aagatatcgc
    14461 tcccgcggtc gggtgtcaag ccgtacccaa tcggcagatt atcccgttgt caacctcata
    14521 actcgacgcg ccaatcctat tttctccagt cgaatgcaaa ctgcccacat aaccgggaac
    14581 accgaaatcc cctaaagtat agcgactggg accttagagt taggccacat acctccttag
    14641 actggataca gtctgcgcgg gccggttttt tgaaacgtgg ctactgacaa ggcgagcgtg
    14701 gtggcggttt attgcctttc tagtatccgc gtccttatac tggatgcgga ttgaggcagg
    14761 cattaatccc cggaggacca cttatggcct ggccatttct gcgaatcggt aaccaaacgg
    14821 cgttcagtgc ccatcacccc gacgatccct ttcgtttcgc gatcgagcag ggatttgacg
    14881 cctttgaatg gttcaacgac aagcggcacg gctgggggtg gtgtcaggaa gacgtagacg
    14941 attacgatcg cgagcaaatc aggcaattgg gcgagaaaca ctccatcgtc ttcagtgtcc
    15001 atgcccgttg ccaggccaat atcaacacgc ctcaggggag ggatgacgtc cttcgcagca
    15061 tcgaatttgc taaggacatt ggggcggtgc tggtgaatgt ctattatcat ggcgaccagg
    15121 ggccccaggg ttttgcggcc tcgttggccc cattggtgga ggccgctgcc cccgctaata
    15181 ttcgtatcag cgtggaaaat acggtcttcg ttagcccgca cgaattgaac gaactatttg
    15241 ctgcactcga cgccaccgat tggtgccgtc ccggcgcggt gggggtttgt tttgatatgg
    15301 ggcatgccaa tttatgtgcg gccacccggt acgactacgt gggttttctt aaccagttgg
    15361 aatccaacgt gcccattatc cacctccatg tccacgaaaa ctggggagac ggggatagcc
    15421 atctgccgct tttcactggg ccggcggctc aggacgactc cggcgtgcgg acgatgctgg
    15481 gtattctgcg agaaaggggt tacgatggtc ttatcatcat ggaacaatgg ccccagccgg
    15541 ccgatcaatt gattactacg cgcaaccgtt tgcgagagct tctccacgct gtgtcgtagg
    15601 ctatttcgcg aaagattctc cccgctgagt gctaccccag tatggcatcc agacttcggc
    15661 tgagtgtaac gctgagacgg taaaataggg gttctacgca ggctattccg gtaaatcggc
    15721 tttttctggt cctggatgtt cccaagaagc gatacactcc atacgagcag ccggatcagg
    15781 atggccggtt gtctatggtc tgtagcagtt ggttggtcaa attgcacttg agggatgcca
    15841 tgaacgcacg gatgtggcta ggcagtgtgg cggtttgcct gttgacggca agtgcggcgc
    15901 ttgcgcaata cgggccttac tatggagcgg ggacagcgcc agcttggcaa ggtgtgatgc
    15961 ccactgggta tttcgaggga aacaatggtc aggctttggc gccaacgccc gatcagggta
    16021 ctgcgacgac atcgttgggg ccttgtccca cagggtgttt gcccacgtgg caggtctggg
    16081 gagagtacct gtatctgcga ccaagcgatg ctgatgtccc ttgggcggtg gccattaacg
    16141 gggctattca gcaggggagt gttcccattc aagtgagtcg ggtggcaaat gccgacattg
    16201 actttgactc cggcttccgt ttcggtgttg gtcgcgccct tgacgagtgt gccagtgttg
    16261 gcgtcagcta cacattcttt gaaagtcact ccaacgattc cttagccacc gatgatccgt
    16321 acgtccttcg ctcgctggtg gcccatcccg gaacactgac agcggctgcc gattatctcc
    16381 gtggaatcgc cagcaacgac gtggacttcc agttgatcga tgctgactac cgccatgtgt
    16441 tccggagcaa ttgctacggc tccatgaact ggctggttgg tgcccgttac gcccagttcg
    16501 agcaggattt caacgcccta ttccgcaaca acgggattga aaccgttctc accgaaagca
    16561 ggttctacgg ggccgggttg cggttgggtc tggaaggcga acgccactcc cagaagtgcg
    16621 gcttgctgat ctatgggcga agcacggcca actttgtggc aggcgatttc cgtgctcgtt
    16681 accaacagag ccagtcctac gacgtcattg aggtggacac ccgtttgcgt gccagccgtg
    16741 ttgtccctat cctcgacctg gaattgggta gcggctgggt aagccaagat ggtcgttgca
    16801 tgttcactgt gggctacatg gtcagtgcct ggttcaacgt cctcgatgcc gacaccttca
    16861 ttcgcggcgt ccaggccaac gacttcagcg acctcgacga tacggttgtg tttgacggtc
    16921 ttgtgggtcg ggccgaaatc cggttctgat ctcgcccctg actggccaac gtagcacagc
    16981 acatccttca agtgcaatct cacccgccga gttttctcgg cgggttactt ttttcatgga
    17041 gcatctcttc gccaaaggca cggtggcgtg tgttcccggt tgttgtaact ctcggcgagg
    17101 cgcggtataa cctttcctta aattgacagt ctcaagatga atcggggact tagagcccat
    17161 cccataatct cccctcgccc gttcggtggg aaaggggtcg ggtgtgaggg tagctggagt
    17221 cgctgccggt tttctcgatc gactccgacg accgcttgcc ctcaccccaa ccctctcccg
    17281 gagggaaagg ggcttttgag ataggctctt gtgacgcggc gaacaccagg agagtcaaca
    17341 ccctttgacc ggagcaagaa gcttagcccg cctttttggc ccacatgaac gaggagcatc
    17401 gccgtgcgtt ttatgagcca tcgtcgtgcc ttcccttgcg caatcatttg cggtcttgtg
    17461 cttggggtga tgtccatcaa gagctcatcg ctggcccagg atttttatgt gtcgccagag
    17521 ggaaatgatc gttggtcagg caggctggcc actcccaatg cggaaaaaac ggatgggccg
    17581 ttcgcctcgc ttgcgcgagc ccagcaagcg gtacgggaac ttcgcaatca acaacccgac
    17641 cgcgatcggc cgatcgtcgt agccatccgc ggtgggcggt atttcctcag cgagccacta
    17701 cgttttggcc ctgaagattc cggaacggag aagtcaccca cgatttatcg ggcacacgaa
    17761 aacgagaggc ctatcatttc gggtggagtc tcccttcgag gctggacagt ggacagccag
    17821 ggtcgttggc atttgatgct cgacgatgtc aaagcgggaa aatggaattt cacgcaactg
    17881 tttgtcaacg accaacggcg ctatcgaccg agattgccga aaaagggtta ttacaccatt
    17941 gccgagcgcg tgcccccacc tgcagacaac cccgtcaaag gtgatgaccg tttgcaatac
    18001 gccgaggccg agttcaatcc gcagtggtcc aatttgggcg atgtggaagt gaatattttc
    18061 caccagtggt cgatgtcccg catgcggatc gccaccattg atgcgagcaa gaagcttgtg
    18121 gtgttcagac gcggaacggg tttcgattcc cattgggccc attttcttaa gggaaaccgt
    18181 tattacatcg aaaacgtggc agatgccctc acggaaccgg gggagtttta tctggatcaa
    18241 aagaaaggcg aattgatcta tattccccgg ccaggcgaag acccgcaaag ggccgatgtc
    18301 atcgctccgc gtttggagaa cctcgtcatc tttgcctgtg acggcgccaa caaacgatgg
    18361 gtggagcatc tccagttcca tggactcatc tttgcccatt ccaactgggt gtgtcctgaa
    18421 aacggacagt tctatcccca ggcggaagtt gggctagatg gcacaatctc ggcaatcggc
    18481 gcacgcaacg tcgtcttcga tgcctgcgcg gtgatccaca cgggcactta cgccatggca
    18541 tttggtgagg gttgccgcaa ttgccgagtt gagaactgcg aactagtgga cctgggtggt
    18601 ggcggcgtta agattggcca tgcgggctcc gccaactggc agggggccag ccaaaggccg
    18661 cagggcgagg aggcccttgt ttcgcacatc actgtaaaga attgcaccat tgcccacggt
    18721 ggtcggatcc atcctgcggc ggtgggcgtg tggatcggcc attcagccta caacacggtg
    18781 gaacacaacg acatcttcga cttctactac accggcgtct cggtggggtg gacctgggga
    18841 tatgccgaca gtccggccca tcataacgcc atcaattaca accacatcta caacatcggg
    18901 cagtatgtgc tctccgatat gggtggcgtt tatacccttg gtgtttcacc aggcacgacc
    18961 gtcaatcaca acaagattca cgatgtgtac tcgtttagct acggtggctg gggactgtac
    19021 acggatgagg gttccacgca catcgaaatg gcgtacaact tggtgtatcg cacaaagacg
    19081 ggcagctttc accaacatta cgggaaggag aaccgtatcc ataacaacat ccttgtcaac
    19141 tcggtcgagc atcaaattca gcggacccgt accgaagaac acatttcgtt cttcttcgag
    19201 cgaaatatcg ttgcatggga caatgatagc ccgctgttgg gaagcaattg gaaggacaac
    19261 aatttccgca tggattacaa cctttattgg cacggtggca agccggtcgt tttccctggc
    19321 aacttgacgc tggaacaatg gcaaaaagaa cgcggtcagg acgtgcactc gatcattgcc
    19381 gatccgaaat tcgttgatcc ggccaaggat gattaccggc tccagccgga ttcaccggcc
    19441 ttctcgctgg gtttcaaggc ctttgaccca aacgaggcag gtcggaccag cccgccgcaa
    19501 ctgacggcga atctgccgga ggttcccacg ggtcttgcca agggagagta aaacgctgtc
    19561 gtttacaata acacattttt ggagggtctg agtatgacca cggtgcgctg catcattcgt
    19621 gccctttggg taaccagttg tctcgcttgg ctagtgcttt gtcagggagc ggtgaagggg
    19681 gaggaatcga cctcgatctg gagcgctcct ccggagtcgc tcgccaaatg gcaggccatg
    19741 cgctttgggt tgtttattca ctggggccct gtcagtctta agggcacgga aatcggttgg
    19801 tcacgcggtg ctcaggtccc catcgaggaa tatgacaacc tgtacaaaca atttaacccc
    19861 acgaagttta atgccgacga gtgggtctcc atcgccaagg aggccggcat gaagtatctc
    19921 gtgatcactt ccaagcatca cgatggtttt tgcatttggg actcggaata taccgactac
    19981 gacatcatgt ccacgccttt taagcgggac attttgaagg aactgtcgga ggcctgccgt
    20041 cgtcagggga tcgtcttttg cgcctaccac tccatctgcg actggtatca cccggattat
    20101 ccgttgggct cacctggtgg ccggacgaaa aagcccaatc ccaatatgga gcggtatgtt
    20161 cagtatttgc acaatcaaat agccgaaatt attcgcaagt acggtccgct gggcgtcatt
    20221 tggttcgatg gcgattggga agagccatgg acccatgaac acggcctggc actttataag
    20281 tacatccgca cattgcagcc ggatatcctc atcaacaacc gtatcgacaa aggtcgccgg
    20341 gggatgaaag gcgacaccgc agggccacag tttgtgggcg attttgatac accggaacaa
    20401 cgcgtcgggg ccttcaatcg ggagcgcccc tgggaaacct gtatgaccat ctgccgccaa
    20461 tgggcttgga agcccaacga tgagttgaaa acgctctcgc aatgcttgga gatactcctc
    20521 cgcacggtgg gtggagatgg gaatctcctt ttaaatgtcg gccctatgcc ggatgggcaa
    20581 atcgaaccgc gtcaggtgga acgcctcaag gagatcggtg cctggctgaa gaaatacggc
    20641 gacggagttt atggaactcg gggtggtccc ttcaaaccag gtccctgggg ggcttccacc
    20701 tgtaaagaga acaagattta cgtgttcatc tttaactggc ctaaagaggg accgcttgtt
    20761 cttccggcca tcaatcagaa gattgtctct gcggttgcca gaaccggcgg agatgtgaag
    20821 gtcgaacaaa gcaacacgca gatcacggtg agtctctcgc ccgagcagcg cgaccccttg
    20881 gtgacagtct tggagctaac cgtcagcggc acagccttcg atatcccgcc ggtggccgtg
    20941 ccagcactca gtggtgccct cagtgtgaac aggcccacca aggcctccaa tgtctttcag
    21001 aagcaggtcg cgcggtttgg tccgcaaatg gccgtggatg atgactccga aacccgttgg
    21061 gccaccgatg ccggaacgca tagcgcttgg ctcgaggttg acctggaacg gccgtgcgtt
    21121 gtggatcgag ccgtcatcga agaggccttc gcccctcgcg tccaggagtt tcaaattgag
    21181 tgtgcccaag gcgacgagtg ggtcccgtgc ttcaaaggga cgaccatcgg cgagcgcggt
    21241 gagtttcgct tccgggctgt gacagggcaa cggtttcggt tgaacatcct caaggcaacg
    21301 gaaggcccga ctatctggga gttccagctt ttcgggagcg agaagaaaga ataaagaggt
    21361 cttttgccga tttatgtccc gctcgtggat ccgcgttgat tcgctggacg atcctcggct
    21421 tgccccatat cggaatctcc cggaacgcac gctccgtggc gagtcaatct tcatcacaga
    21481 gggttggctc gtcacggagc gcttgttgaa aagcacctac gaaacggagt ccgtcttgct
    21541 ggccgaggat tgcgcggccg aatatgccga tcaagttccc gattcaattc cgctttacgt
    21601 tggaccgcgg gaactccttt atcaagtggt gggctaccag tttcatcagg gcgtcctcgc
    21661 cgtgggccga cgacgtatct tgccgtccgt gcgggacgtt ctaccgcccc ctgatcgacc
    21721 gggccccctc atggtggtcg tttgtccaca gatcacaaag cccgagaaca tcggcctggt
    21781 gtttcgctcc gcggccgcgt tcgacgtaga tgccgtcatt ttaggtgagc ggtgcgctga
    21841 accgttttcc cgacgcatcc tccgcgtttc tatgggcgcg gtgcttcacc tgccaatggc
    21901 aaagaccaac aatctccggg aagaattggc gatcctcaag cgggaatgga atgtggcctt
    21961 ggtggccagt gtactggacc ccacggcagt ttcattgcca gacttttcgc cccccgaaag
    22021 agttggcttg ctattggggc atgaaaccga aggacttcgg gaagggtggg ccgatctgtg
    22081 cgattactcc gtgaccattc ccatgtctag tctggtggac tccctcaact taggggtggc
    22141 agcgggcatt tttctctatg aattccgaag aaacggccta aaacggcgat cggcttgagg
    22201 gattcccctg gtgccggacg gctgtgtcct actttttgag ttaagctttt ctggacgccc
    22261 ccaaaggcga taggtgcgta cgcgcctaag gtgtgccatt tcacaaatct taaccacgat
    22321 cggaaatccg aaagacgaaa gatgggcagc ggcgccatcc tgaacgactg ggattcattg
    22381 tgggaaaacg atatccgttt tccccagtgc cactctcatg agcagctcgc gcgtcttttt
    22441 cccgaaatca acgtaagttt tgtggcatct ttcaacgaga accatgcgcc gcttgcgttc
    22501 tggtgtgacg atcactggcc agaacagccg gcgatcaccc tcaagaatct ccgttcattc
    22561 ctgccagaga cgatgacccc aactgagtgg aggccgtttg cctacggtga aggccagaat
    22621 atcctcggat tcgtcactcg gtttcaagcg ccagaagaat gctaccagat gaaatctcca
    22681 ccggcgggtt tcgtcgtggg attgtatcgc ggaaccgagc aggctttctg cgcagcagcg
    22741 ccgctcttga ttccttttgc ccgcgcgctg ggcctatcag cgtggctggc agatcgtttg
    22801 ctattgagtt gtacgaaaat cgaacaaatg gagcaggaga aaatctcgct taagcagtca
    22861 cattcagaag ccgtgagcat ggctcttgag gagcacgaat tggttcttct ggccgaagaa
    22921 aaacaaaagg tcttgcaaga actcatcgag gccaaggaag ccgcctatcg ggcaaagagc
    22981 cagtttttag ccaatatctc ccacgaattg aggacgcctc tgcacgccat tctcagttat
    23041 gctgcatttg gcataaagaa gatcgaaacc gcggacaagc ccacccttct tcggtatttt
    23101 cagcaaatcg agacaagcgg aaaatccttg cttgcgtttt tcaatgatct tctcgatctc
    23161 tccaagctgg aggccggcaa gatgcgttac gagcgatcgg tctccgaggt tgtctggctc
    23221 atccacagcg tcttggaaga gttccgttcc ctggccgagc aaaaacgtat caaactggaa
    23281 tacattgggc cgcagtgctt tcgcaccgaa ttggacccca cacgatttgc ccaggtgatt
    23341 aggaacctca tcagcaatgc tgtgaagttt acacgagagg acggacatgt ctgggtaacc
    23401 ctcgttcccc aggaggacca tttcctcctc cgcgtcgatg acgaagggtg cggaatcccc
    23461 gatggtgaac tggacagcat cttcgagagt ttttaccaat cttcgagaac aagaacatcg
    23521 gccgggggaa cagggctagg attggcgatt tgccgggaaa tcgtcttggc tcatggaggc
    23581 cgcatctggg cggaaaaccg acccgaaggt ggcgcgcgat tctcggtttt ctggccaata
    23641 actcggacaa cagaccactt ggattcacaa ggcaaccccc acgctcctcc cgcaactgtc
    23701 gtaggcgcgg acgatgaagc gtcgagttca aatacgcaag acgttctcgt caccgccacc
    23761 gctgaggcgt agcccggcgc cgcagcaaat tcgtgaaagg ggtgcacgtt ccattgaaac
    23821 gagcagggtt ctcgcgcaaa atagcccctg atcaactaaa atgttgttgt cggcagattg
    23881 tacttgaaag cgaatcgctc gcgtgctgtc gattgtttgt gtaatcagtt tgcccatatg
    23941 tgggaggcct tgcccatgaa aactcgcata attctctgtc gctttaatca aatcgtggta
    24001 tcgattccct cgttgatgct ccttttggtg gcaatgggcg ttggcagaga cggtatcgcc
    24061 tccgaaagcc agcccggtca gcaagtttcc aaaaagatcg tgtttattgc gggccgaccg
    24121 agtcacggct tcggcagcca tgagcataac gcaggatgtc ttttgttggc caagaagctc
    24181 aaggaagcct tgcccaacgt cgaaacggtt gtttacacca acggttggcc ccagcagacg
    24241 gatgccctga cggacgcgga tgcgattgtc atctttgccg acggtggtgg tggcaacatc
    24301 atcgcacaac acctggacga acttcagaag gcgattgaca aaggagctgg tctagcggtt
    24361 ttacactacg ccctggatat tgctgcgccc gagggacgcg aaaaactaaa atcctggatc
    24421 ggtggctatt atgaaacgta ctggtcggtc aatccgcact ggcgagctga gttcaaggct
    24481 ttgcccgagc accccatcac ccgcggtgtc cgccccttcg ccattgacga cgaatggtac
    24541 taccatatgc ggttcgtcga tgacacgaca agacttgtgc ccattttgac ggccatcccg
    24601 cccgacagca cgcgagaaag acccgacggc ccccacagcg gcaatccgac ggttcgctct
    24661 cgaaaaggca tgccagagca cgtggcgtgg gcttatgaac ggcctgacgg cggccgcggt
    24721 tttggtttta ccggagggca tcaccattgg aattgggcgc acgatcagtt ccggacgctc
    24781 gtcctgaatg ccatcgcctg gtgtgcccga atcgaaatcc cagagcaggg cctgcgcacg
    24841 acgactccca cgatagagga gttactggcc aatcaggatg agccgccccc ggcgaatttc
    24901 cagccggaaa cgatcaaaag acagatcgag agttggaatc agcaatagtc atcgtctcac
    24961 ggcgaagaga gacctaaccg cgtgagaaaa cgaaacgtcg gcccatgtgc cgcagtcgag
    25021 gacgttgagc tgctccgaga atgacctgtc aacctgtacc tttcttccgc tccagccgtg
    25081 cgattttgtg gccggccttg ctggtgacct tgagcttggc cctgggcagg gccgctttcg
    25141 gcgaagagaa ggcccccgaa gccgcaccgc cgcagggtct tcgcatcctc tttcttggag
    25201 ataacccatg gaatggcggc acgcttcgcc gcgtgtttga ggcctttgcc gcgacctaca
    25261 ggcgaaacat cgagatcacg gaaatccctg cgaaagaccg ccccctgggc gaggtcatcg
    25321 ccggtctaat tgagagcacg ccggaaaagg gacgcttcga cgtcgttgtg gcgtttgacc
    25381 atcccctgcg gcaggtggtc cagcgggatc gggccgaact gatcacggag atcgatacgc
    25441 tcgagggact ggccccgaga atcgtctgga tcctttcccc gtaccgaaaa ccgacactcc
    25501 gtgtggagac cgagcggcaa aaaatcctcg ggcgattgcc cctgcctcaa ggattctcta
    25561 ccttaccgct gatccggctg ttccatcaaa ctcggaagtt ggccggtggg gcggcctggg
    25621 tcaacgacct aagtttggat cgccccacga gcttcagcga ctatctcacg gccgccctcc
    25681 ttgcccaggt cctttggggt cccgctcctc aatttccgaa cgacattcgc atcagcgata
    25741 cggccagcct acgcatcccg gaagaggcgc tgcccgtgct cgccaaggta cttgccactc
    25801 tggaaataac tctccccaaa ggcccggccc ttgccaatgc gttcgccccg gaactgagtc
    25861 cgccccagcg gtttgtcggc aacggcctac ctctgacgtt gacctctggc gtaattccag
    25921 gtttttttga ttggaatgcc gacgggcgat tggagcttgt gctgtgtgga cgagaaacgg
    25981 gccaatggga aatctggacg tggccaactc ccgacaagcc gttgtcgttg gcccaggctg
    26041 cgcgatttcc cgccgaaacc gtgaacccgg acttggcggc gatgatcaag gctggggcaa
    26101 ctgtctttca agtggtggat ttcgattatg atcgccaccc cgatttgctg atcggaacaa
    26161 atgagggcaa gcttgccctc tgccgcggcc aagcgggtat ggtatttggg cggccagaag
    26221 tgtgcatgct ggaagacggc aagccgtttc aggtccccta tttacatcgg gcatttctcg
    26281 gtgaactaac caaagatggt gtctgggacc tcgtgctcgg tgatggggca gggcaggtgt
    26341 tcctgacatc cctggtggag cagggaggtc gttttctctg gggccagccg cgccccgtta
    26401 ccattgaagg acttaccctg caagtcgatg gtgaggcctc gcccgtaatc gccgattggg
    26461 accttgatgg cctgcccgat ttgattgtcg gctgccgaaa cggaggcgtc ctttggattc
    26521 ggaacgtcgg ttcaaatgag cagcccgcct gggcaccacc ggtcagcctc gtctggcccc
    26581 cggaagaaga agagacgttg ctcattccct atcgaaatgg aatggaactt tggacgccga
    26641 caccgggatg gtcaacgcaa ccatccgtgg ccgacctgga cggcgatcat ttgcccgatt
    26701 tgatcatcgg cgattccaat tgccgggtcg tcaagtatcg agattttacg gcagaggaac
    26761 aaaaggaagt cgatgcttta ctcaagaaac gcagggaaat cgtcgagaaa ctcagccaat
    26821 caccaccgga agaaatcgcc agacaaaaag cggcactttg ggaaatcacg cttgagttga
    26881 ttcaaaaatc aaccgataga cgctacgaac gatgcggctg ggtttggtac ctgcggcgcc
    26941 aatcgttggc tacccccgat gaataacacc agcgccaacg ttccaagtgg catcatattg
    27001 ccatcttccg aagcgccaac gttccgaacc gccaacgtag gccggtgagg gagtgaaacc
    27061 ggttgacgtt cacttgacct gacagcgcaa attgtcaaaa attacaatcg agctgaagcg
    27121 aagaattgaa acaacagccg ggcaagagcc tctggcagag tcaggtttta tttctctccg
    27181 ttttcaattt gggcggtcca attaatcaaa tcgataggtg caacgatggc agagcacggt
    27241 aatcggcgtc agtttttgaa ctatggacta ttaggagcca tgggcgctgc cgcggcttcc
    27301 agtttagagg aacgtatcct cctcgccgcc cttcaggagg gcgcgccgga aaaggcggcg
    27361 cagactccgg tggatgaccg cccgatgcct tgcggtcgta tccgcaatct caccattagt
    27421 cggatgttaa ttggaggcaa tctcatcggc ggttgggcgc atagccggga tttgctttat
    27481 gtctcccgat tattcaaggc ctataacact gaggaacgca tattccagac gttggccttg
    27541 tgtgagtccg tagggatcaa cacagtgcag ctcgatccgg cttgcatcaa tgtctttcat
    27601 cgctacttga aagagcgagg cggtaagatg caggccctgg tgtgcatcca ccccgatgca
    27661 gacgcgggaa aagtccggtc gcaaatcgaa gatttgctca acaaaggcgc cacgtttttg
    27721 tacacgcatg gtgaggtgac
//
LOCUS       DAOSUT010000040        27616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1140_length_27616_cov_10.465331, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000040 DAOSUT010000000
VERSION     DAOSUT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27616
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_27616_cov_10.465331"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PLD05_05340"
     CDS             complement(<1..173)
                     /locus_tag="PLD05_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOP76892.1"
                     /translation="MGLASALTTALTGLTAAETTIDVVGNNLANSNTVGFKASSAAFA
                     TQFLQTLALGAMPT"
     gene            complement(326..733)
                     /locus_tag="PLD05_05345"
     CDS             complement(326..733)
                     /locus_tag="PLD05_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOP76893.1"
                     /translation="MIVNSAAATRQATNQNPTGHDAFRDLDLNSFLSMMIAELQNQDP
                     LNPMDNAQLLQQVSQIREIESNLRLVSTLESLVRAESLASGTNLIGRTVKGLDTNGKE
                     VVGVVEKVSADEKGFYVHFDNTTVSMRNIKEVI"
     gene            complement(784..2421)
                     /locus_tag="PLD05_05350"
     CDS             complement(784..2421)
                     /locus_tag="PLD05_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOP76894.1"
                     /translation="MLDRIGFAPEIGIIDSLSAIQTRGLGFSDQAEEVGSSFWNVLAS
                     EIQGTEESSRPSVTARCAETIPGGTKNGAHQGESVADTRDEKLIEPCPGKMPKEGAAQ
                     EHDQALHDDSDVERSGKNSGEGAALGQDAEPQTSTNPYQVVVCCQKGQGAEPGTLVAD
                     TLVKESAPETSHMQDKASDVTADVVGIGQSVLPLTVQLITSEENSTDQIEETGLGFES
                     EVKPFGKKLNKGGPQAQIMPSAVPSSEAVEEANQTGGTVNQERATGDSETVSEQLRSA
                     FPAVAVSGGQSDVIGQTVSRKKESAVLSGQDNGANPTVSQKLTSGTTEMTPQNAGLVT
                     SYDPSVNASLAVSNERDERVKTVEGAKQEGAGFVFTRKDLARTEVSTGPKTGEQSLAQ
                     GPSMRADQAEQIITVQRVAQAIRLAHERNGEIRLRLHPPELGALRLQMRVQEGVLTAR
                     LEVETLAAREVLLDNIAGLRERLAEHQIRVEHFDVALMNDSLGGQTKGREEFLGSSHP
                     SRHRGASAEPKSSEEVGGQIPSIPTRRLGDRQFDVFI"
     gene            complement(2531..3181)
                     /locus_tag="PLD05_05355"
     CDS             complement(2531..3181)
                     /locus_tag="PLD05_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76895.1"
                     /translation="MMRFLETILSVLLYFFAAMAIGALCLFIHLWFAWKLTPEKIRTA
                     IAAARGTLPPPVTAIAGPVGPKEPAEEPSYSDILAKRASSYRDLELKEKMLASLKDEL
                     LQWETTLSKKEEVLGQQEKAFQERLAQMKAEAESAGLEAVRRTLESLRPTQAKQLIEE
                     MLAQKEEDQVVTLLQGMSDSKRARIIAEFRTEEEMARIAEVLRRLRQGGELGTSQQ"
     gene            complement(3178..3630)
                     /gene="fliJ"
                     /locus_tag="PLD05_05360"
     CDS             complement(3178..3630)
                     /gene="fliJ"
                     /locus_tag="PLD05_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOP76896.1"
                     /translation="MRRFTFRLETVLRIREQERDERKRELEQAYAARDILLRQRDALE
                     AERQHLGAALRESLQQGSIDADQMLAWRRYEAVLNAQREYLTKQLSAIEGEIEKRQLA
                     LMEANREVRILEVLRDKQRTAHEKQERAREGKKLDEIANRRSHGLREP"
     gene            complement(3682..5073)
                     /locus_tag="PLD05_05365"
     CDS             complement(3682..5073)
                     /locus_tag="PLD05_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOP76897.1"
                     /translation="MIDLKSHLERIISAEIEGSVTQSLGITMIAHGFSVPVGSLAEIE
                     IPHGGNVLAEVIGFKNDSTILFPLGRPEGVRPGCRVRLARTLHWLRVGNDLLGRVVNA
                     QGHVIDGKAQPFLKHRVLFDQPAPHPCERPRIQDPLPTGIKAIDGLLTCGRGQRLGIF
                     AAAGVGKSVLMGMMARYTAADVIVIGLIGERGREVNDFIERDLGPEGLKKSVVVVATS
                     SEPAILRVQAAYAATAIAEYFRDQGKDVLLLMDSLTRFALAQREIGLAAGEPPTTRGY
                     PPSVFALLPRLVERAGRTPRGSITAFYTVLVEGNDPDEPVADAARGLLDGHIWLSRSL
                     ANRGHYPAIDVLESLSRLMPDVVEPRHYQSALQVRDLMGVYREHEDLITIGAYRRGSH
                     PKVDLSIQLRDTINAFLRQNVGQRAEFNETRDSVIKLGEQIQETLRAATTQQSGKEST
                     GRHAGVNGPPVRS"
     gene            complement(5104..5778)
                     /locus_tag="PLD05_05370"
     CDS             complement(5104..5778)
                     /locus_tag="PLD05_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOP76898.1"
                     /translation="MPMEARSSVIKVHDGAGVAQATPFNFEDMAERARAYLDGVRKQA
                     VQIVAQAQKEAAEIRKRAEIEGRRSGTEQAERVIRSELEKSLTTVLPALREAVSQINQ
                     ARQAFLAEWEKSLLRLAGAIAVKLVRQAVIETPEVAVPLIRETLELAAKAPRVRIRLH
                     PEDEKALRPVVTKILQELSPAAVGEVLPDPAITRGGCRVETDFGSIDQQFEKQVQRII
                     EELTGQ"
     gene            complement(5837..6877)
                     /locus_tag="PLD05_05375"
     CDS             complement(5837..6877)
                     /locus_tag="PLD05_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOP76899.1"
                     /translation="MAHAGLRKAAIFIDCLDAKTADLVLDRLPREYADQVRSTWMALG
                     RIPAEEREQVIREFMQTTAQPSRETTLTSTPPQWEGTELILQSSMNAPAKKNEDTEGS
                     PPFRLLREAEADKLVGILQGERPQTIALVLAHLPPHQAGAVLVRFPPAMQVDIIRCLI
                     SLEETDPEVLREVDRALESKLSQLVAIQRRRIAGITAVNGIVHQSQEHVSQQILDNLA
                     NFAPDLAERLSPIPKIDFNQFIRLDESALATIFEKADDELVVLSLWGAPLGLVEKVLQ
                     QFPTWRAEAIRRELADLGPVRLSDVEEARRRLAEYASKLAACRRIRLPPELQSHPVAT
                     GSARSPGSTYGL"
     gene            complement(6900..8540)
                     /locus_tag="PLD05_05380"
     CDS             complement(6900..8540)
                     /locus_tag="PLD05_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76900.1"
                     /translation="MDFINKTWDQLKDVFLSLTPGTRVTTVLLLVVVLVSLGYLFTYS
                     GSGGSTYLMNGAHFSPTDLPAMEAAFAAKGLNDYRIEGTRIRVPLGKQDKYVAALAEG
                     NALPYNYLDILEKTINESSAFESRYKLEERTKIALQKELSRVISQMAGVQNAVVMFSR
                     LNQGGLRRETITTASVAIKPIGGQELSEQVVRSIRYYVAKSIGGIKPEEVVVTNMDTG
                     ANYYVTNDEPLQAAEGTYALKRSWETYFAEKVKTALGYVPGVLVSCNVELDPTKYQRQ
                     EQVKIDPKAVPLDVTETTKTRQSTSDPGGRVGYVAQGNTPRSLSGGNTPTPSETEEET
                     QRTEKNTVGQTVIQQETAGLLPKRVTVSVAIPASYFRRIWEQSQPTQANNPQGPTPQE
                     LDKIRTEEMTKIRNHVMALLPVVENVQDPSQLVTVTDFVDMPLPPPQPVSIVEQTFDF
                     LRVYWPTLGLMGLVVFSLIILRSAVRATPSAPAGVAPAVAAAAEETAAQEEQKKSEKK
                     KWRTSETGKSLRDEVAELVADDPETAANILKTWIGNPI"
     gene            complement(8625..8921)
                     /gene="fliE"
                     /locus_tag="PLD05_05385"
     CDS             complement(8625..8921)
                     /gene="fliE"
                     /locus_tag="PLD05_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOP76901.1"
                     /translation="MNLIRPISPSSVTPVGPTGPGRVTQSGEFKDFLLRSINEVNSMQ
                     QEADRAVERLITGEDVTPAEVLTAVQKADIAFKLMMQIRNKLVQAYQELQNIRV"
     gene            complement(8921..9343)
                     /gene="flgC"
                     /locus_tag="PLD05_05390"
     CDS             complement(8921..9343)
                     /gene="flgC"
                     /locus_tag="PLD05_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOP76902.1"
                     /translation="MFSALDISTSALVAQRTRMTAIAGNIANISTTHNELGQPEPYKP
                     RYVVFQADTSIGQYGASGVKVAAVRVESDRPPRYKYEPGHPDAIQSGPMKGYVAYPDI
                     NMMTEMTDALEAARAYEANLGTIEITKDLTQQTLRILA"
     gene            complement(9449..9862)
                     /locus_tag="PLD05_05395"
     CDS             complement(9449..9862)
                     /locus_tag="PLD05_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOP76903.1"
                     /translation="MWNGLFTQTTIPLLSAVADFSQVRHTVLAGNLANLDTPGYQAVD
                     LPVQDFQGYLKEAVAARHGRGPSMSPGDAVYLRRASSPQWSKEARPILRHDGNDISLE
                     QQVSEMVKNQILHNTAITIMTNQFRLLLTAISEHV"
     gene            complement(10077..11501)
                     /locus_tag="PLD05_05400"
     CDS             complement(10077..11501)
                     /locus_tag="PLD05_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP76904.1"
                     /translation="MTRDKRTELNYRKYDTQPGTVLVVDDHTPSRQAIADILRQAGHH
                     VEMCSSAAEAMSALNRADYDVVVTDLNMPGMSGLDLVRYVEKQQLRTRPIMVTAYATV
                     SSAVEAIRHGAFDYIEKPFDANQLERLVAEAVRHSRLRLAGQANRTGDHTIKAVNLVG
                     SSSLMTELRQRIAQVAGTSETVLIYGESGTGKELVARAIHVQSSRAAGPFVSVNCPAL
                     SAHLMESELFGHEKGAFTGADCDRVGRFEMANGGSLLLDEVTEIEPPLQAKLLRVLQE
                     KAFERVGSSRTLHVDVRVLATTNRDLEKEIAEGRFRQDLYYRLAVLPIWVPPLRQRKE
                     DIPELITHFQQQCAQRLARPVPELDDSALALLMEYDWPGNVRELENLITRICVLTTET
                     TVRADQLRKWLPNVTARPKFENIPVGMSIQEMERYLIEATLERFGGHREKTAKALGIG
                     VRTLTEKLRQYGYAPREKRFSRAG"
     gene            complement(11557..12411)
                     /locus_tag="PLD05_05405"
     CDS             complement(11557..12411)
                     /locus_tag="PLD05_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP76905.1"
                     /translation="MNQKTDLELSAETDLGTILEAWHAATIRLEQTHEALQAEVRRLT
                     AELERKNRELARKDRLADLGRMAAHIAHELRNSLVPVALYLSLLERRTEDEGSREILH
                     KVIEAVQAAETTVTDLLQFAADREPQSVLFPLLGLVEEVLAGLEPQIQAQQIKTVVEV
                     SQALYVWADRELMRRVLLNLVLNALDAMPHGGTLTIEAHAEEGDLWLRVSDSGSGLDE
                     EHLHRIFEPFYTTKPNGTGLGLAIVECLTELHGGHVQASNNPEGGAQFVVVLPQRAVL
                     SGHQQEGC"
     gene            complement(12486..12935)
                     /gene="flgN"
                     /locus_tag="PLD05_05410"
     CDS             complement(12486..12935)
                     /gene="flgN"
                     /locus_tag="PLD05_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOP76906.1"
                     /translation="MPHSANVYESLADLIVQKHRCLKRVHELGQRQMGCVTSGDIEGL
                     LEILAVKQRVLGDLQTIDHRLKEIGAYGDEVDAIPQEWREAIAGVLIESRALLEEILR
                     TESECAHILEQRKQDIAKQLAELHDFVRARTAYQQPGDESGQLDLTL"
     gene            complement(13119..15566)
                     /locus_tag="PLD05_05415"
     CDS             complement(13119..15566)
                     /locus_tag="PLD05_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP76907.1"
                     /translation="MSEPEPSPNTAAESAQAAPVEQGGRVSLRTRLVAMATSVVKSKF
                     RLALAVGIGLTLIAVGAGVGLWFFLRGSPDNSQAEQILSALKRGDYPLVRELTDKVLA
                     TEDVSPDQLRVALSARFFAVAREADAAKGEDRRRLLDVAINYGEQAASVGFTRGWEEE
                     AAFLLGRCLHYRARFAESRKWLREAGKRTVDKQARVYWLLAESHLLDPQGIKEEALKF
                     NEAFLQISRLDVQAKNAGWMQRAKIQIALGNPDDATSSLTKLSPQAAQSPEAQFLRGW
                     IDYELGKRLLTSQEENKAGQDRLRSAAERLERVRRLVDADESLRSRATYLLGLVFEGL
                     GEYDKAIAAWEGCHSEYPGTYEAWAARLRLAQLYQETSRYQQASEAFISCFREIVDSQ
                     EFFNPWVKWSEAQTLVRDALATYKEKQLYEIAMNVIDSLEKSFAEAEVHELRATVLSA
                     WGETLLRQAQSESSDRQINITWAKRCFRLAGDEFTRLARTRSGTRFYTEDLWQAAEHY
                     YRGGSYSRASRVYREYLRNEARRRNPIALLRLGECLLALGRVDEALLVLNECIEFHRK
                     DAASYSARLLAAHAHMEKGSLDKAEEMLLANLSGELAPTSVEWRDSLFTLGEMYYQLG
                     RDQDAIPRLEEAIRRYPEDPRSTKAKYYLAEIYSRLAWNRMMLSPSSSAATPDMATIR
                     NDFLRSLHYYRQVQSDLDFQWTTEEPSEDTRKMLRNTYFAIGRVCYHLGQFAEAVAAF
                     TTITNRFQAEPEVLDAYVEIARCRRAMGQNPDARIALEQAKIVLTRFPNEIPFERTTP
                     FSRAEWATRLDKLLQTP"
     gene            complement(15961..17460)
                     /locus_tag="PLD05_05420"
     CDS             complement(15961..17460)
                     /locus_tag="PLD05_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76908.1"
                     /translation="MRESCGLGRFPRRDVLQMAGLAGVSAMVNTLVARASEDSSAVAQ
                     EVVLPPIEKKPPRIWAVFLYPPADVVLAGKMEDSWAPHQWFTWPGNQFEPEAQQAKFT
                     GVIQKMASDLGIDLVIHPEALYQKAKVDEFIAQARAAELDAVLVINFWNTLSWAAARI
                     ATEAAPTAIVYHSLGSNHQLPPESLRKAEGLYYIHSMENFAEIERGLRAVRAKKMLAQ
                     SRVLRVSGAVKELTESQEPSLGTAIVAIPAAEFNDLFDSISPTPQLQEEAAAFRRMAS
                     KVVDVSEEAMVDAIRAHHAVQHIMRRYGADAITIQCLMLKHRKPCYSFAWHNGQLVPC
                     GCENDINATLTLMLGRWLLDKGGFQHNPEFDTSENLYFGAHCTCAWKLHGPQGPSQEF
                     FVRPFFHQLPKTPALDVQWSPGEPVLLTKYLTGRNTLMCYRGKVISSPTCPPTGGCAT
                     RVLVELEGVADVCEAYAGPHPVLFCGDRGDARCFKAFAKMYRFTLLGNA"
     gene            17961..19838
                     /locus_tag="PLD05_05425"
     CDS             17961..19838
                     /locus_tag="PLD05_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76909.1"
                     /translation="MMSRSILRIAACFLAVVVAVFNEHLLADQQPERPNIVWLTSEDN
                     AIFWYRLYNPDGAPMPNVERLAKEGLVFNNCYSCAPVCSPARSTIISGCYVPRLGAHW
                     HRKILPVTLPAGLHMFPWYLRQAGYYTTNNHKEDYNFAASEKADVWDESSPRATYRNR
                     KPGQPFFHVQNYTTTHESQLFPERPNDQLITDPKSVKLFPYHPDTPIFRNKYAHYFDL
                     HRQLDAQIGTFISQLEADGLLDDTFIFHFGDNGGVLPGSKQYAREDGLHVALVVYVPR
                     KWQHLVPAPRGSRINGVIEFVDLAPTVLNLAGIPIPPQMDGRPFLGPGVTLEELDQRD
                     TAFGHADRFDEKYDMVRFLRKGRFTYWRSYQPFNFDGLYNEYRYKQAAYREWRDLYEK
                     GQVNDRQKAFFEPRPPECLFDLEKDPHETHNLAGDPAYANVLVTMRRLMQERVKSLPD
                     LSFIPEPIFIKESGGDGFGYASKHKARIARLVDIADLQLLPFEEAYPSLQQALASDDP
                     LERYWGIITCSAFGKKAMPLAEKIRRLAESDECSFVRTRAAEFLGLTGLGDPGPILID
                     VLKKIDDPAGANLILNTVVLLRDGAPRLQIDLSPLRNASWFTASGEVSRRVNYLFGQG
                     Q"
     gene            19997..20962
                     /locus_tag="PLD05_05430"
     CDS             19997..20962
                     /locus_tag="PLD05_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76910.1"
                     /translation="MTGFTSWNRRAFLRQFGALCCGGIGLSLNVRGETSGEEARLAFR
                     PEGDGFAFDTGLLRGTLRQQGRPIGMAPLYYKDFSSDLSQPYGVCSHYRLLDAETRYG
                     VAAWDWSGTAELLSDGSVKALWTRDETHPFDLQAHYRWHAPDVLEVLTTVVAAKPLQK
                     FEVFLASYFNGFADSFVWARSSPAASGEATFMKAEQKNGVWQMFPRDELAIKLIQDGR
                     WSRPPHPVNWAIMPFFAAPLAMRRDAETGLVAVVMSRPEDCFAVATPYSGEGHRSIYF
                     SLFGRDLEPEQPVTAEVRLIIGHALIDEVIVDRYRQYAGGEKRNA"
     gene            complement(21055..22440)
                     /locus_tag="PLD05_05435"
     CDS             complement(21055..22440)
                     /locus_tag="PLD05_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOP76911.1"
                     /translation="MATFLRGVTMSLAVAMLLVGLSSCCVAAEDSGLPWKIGLARKDI
                     TPQQPIWLAGYASRNKPSEGVLAPLYATALAIEDSQGNRGVLITADVIGFRKEVAEAA
                     ARAIMEKTGLAREQILFNWSHTHTGPVIGLSRPAGYSMGEEDQKVVETYTRGLIAQYA
                     DLAAEAIADLKPARLAWGVGVVHFPMNRREFTETGVRLGVNPRGYADRSVPVLKITDE
                     KDQLRAVLFGCACHNTTLTGQHYVISGDYAGFAKAFIEEKLPGVMALFMIGCGGDANP
                     YPRGEIEHAKQHGQELGTEVCRVVGEKLSPVSGPLKTALAEVELPLLPPPDREKLESM
                     AKGPSHLSETAKRMIETLQRGESPPSHYKAPIAVWQFGNDLTLVAISGEVVSDFVPLI
                     ENEIGHVRLWIAGYCNEVFGYLPSAKVIREGGYETRGVGTLGFFAPEAEKVVVETVRK
                     LSEQVGRPPLP"
     gene            22779..23825
                     /locus_tag="PLD05_05440"
     CDS             22779..23825
                     /locus_tag="PLD05_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76912.1"
                     /translation="MSCHRFDHAVRGLRLLVIATVVLGTVPWQAMGMELAKTGTRRTV
                     VSIDGTRWLINGQVTYPGSPAEGLLMNVRMVNATFEDLNRQDFDPEANTERFLAHLPD
                     YVAHGIRAITLNFQGGHAGYEGPLNSAFNPDGSLREEPLKRIKRVIEACDQQGVVVIL
                     GCFYQRQDQILKDEEAVKTALVNAVRWIEQQDFTNVVLEVANEFPHRGFDHAILRKPE
                     GQVELIRLAKKTSPRLLVSTSGIGDGRFPDVVAEVADFILIHFNGVPVEEIPERIAAL
                     KRFGKPIVCNEDDKTGSVAAQAAELCVKNGASWGLMLSKLNQYWPFEFHSAADDPVVY
                     AKLKELTTAGDRNK"
     gene            23922..26075
                     /locus_tag="PLD05_05445"
     CDS             23922..26075
                     /locus_tag="PLD05_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76913.1"
                     /translation="MTRTIVWQKKTMRPTHLLHVLVLGFIVPWANVYGEQASPPSLIV
                     AVRSYPEPGKIVASVVVPEVADIAENAELTLQVTLLAPDEKVLLQSHQTTQVGMREEV
                     ILDAAHIAPGEYKIMAVLVGPGSQELLRSQHPVKWPGRSELFRGIRILNNLVWELLNV
                     EATGDAPISGEYVVQLPYDRWLFVRLTLQDGQDDEVKVSLRDSRGVLPLVTHPGFLPG
                     TIEAMRFAKAGRYVIEVTANKGKVKQIVVRAVPEIQHSRYPTSVPYIDQGVRYDWEYL
                     ERHVLPHVNTIITSGPSEEEIRHLQDWKERGGKVIVYTGRPGLHGKKELEASPEVCAN
                     YWASQAGYTLPFSDGILVDEFYHNEDPAYPAYTEAVRLLNTRFPGKAFYPYAAGRFGK
                     DQGSIPFAKVCIEGGGYPCWEAYIAEWPTLKLALNKLREYPYEHVIPFEEKLPGVTKK
                     LIWVFGVFSFPWPYADGYANVNYNAYLDMQFQFIATHPALFGLGGMHIWRSGYCDEER
                     LRWCARLFRHYALEGNTNRLSADPYMLTHIENPDFVEGLKHWEVSPARENSVFATAQK
                     GFGKFIGRYYRGPDSFLVTRRDPEKPNVVRQVIRNLEPGRLYSVKMFTGDYGAFQSGN
                     STRELHAVSLQIKGAELLSGPQYRYQQAFPTRERLGNFTEQKPFWLNLHWLVFRATAP
                     TAELLISDWKAPQNPGGPQDQTLLYTYVEVKPYLD"
     gene            26510..26686
                     /locus_tag="PLD05_05450"
     CDS             26510..26686
                     /locus_tag="PLD05_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76914.1"
                     /translation="MTLYVTIHAGRVILLKDARNGRVTIPSHIGRVAVPGDQISGRRG
                     RRPSLLFTDATRSL"
     gene            26868..>27616
                     /locus_tag="PLD05_05455"
     CDS             26868..>27616
                     /locus_tag="PLD05_05455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP76915.1"
                     /translation="MVQIVRHVALCSVLLFAANGKAFGQNTAQPVVSDEQIPDGSLDP
                     FLGKPDLQKQILFSGERFPNIVVTKKGTVLAVWGSQSVRCRRSEDGGQTWEKEIAIAV
                     PGFHGGGVTVDETNGHIFVFVQAQHPPAPLTVYRSRDDGKTWEQTPVTIHPDQKGHTP
                     SMHMAEHGITLCYGPAKGRLIRPARVYDRPRGYNTAIYSDDGGVTWHTSAPFPDEGTG
                     EGAIAELTDGRLYYSSRKHHFENIADFSWKR"
BASE COUNT         5910 a         7955 c         7314 g         6437 t
ORIGIN      
        1 gtaggcatcg cccccagggc cagcgtttgc aagaactggg tggcaaacgc tgccgaagag
       61 gccttgaagc caacggtgtt ggagttggcc agattgttgc cgaccacgtc aatggtggtt
      121 tccgctgccg ttaagcctgt caacgcggtt gtgagtgcgg aggctagacc catatctatg
      181 gctccttgga ctcgctattg acaatccccc acagagcgtc tctttcaacg tgtatctagt
      241 ttgatctccg tcaccgttcg acgccctccg attcgtaaat caaagttgat tcatcacagg
      301 tattcgttcg tgagcgatcc cctctttaga taacttcttt gatgtttctc atggaaactg
      361 ttgtgttatc aaagtgcaca tagaagccct tctcatcagc cgagaccttt tctacaactc
      421 ccacgacctc cttaccgttc gtatccagtc ccttgacggt tcggccaatc aaattggtcc
      481 cgctggcgag actctcggcg cggactagcg attccaacgt gctcacgagc cgcaagttag
      541 actcgatttc ccgaatttga ctgacttgct gcaagagctg cgcattgtcc ataggattga
      601 gggggtcttg gttctgcaac tctgcaatca tcatgctgag gaaactattg agatccaggt
      661 cgcgaaaggc gtcgtgaccg gtaggattct ggttcgtggc ttggcgagtc gcagctgctg
      721 agttaacgat cattgttcac tccatctcaa cagatttcag atcgctgact gtcttgttcc
      781 atctcaaatg aaaacatcaa attggcggtc tcccaggcga cgcgtgggaa tactgggaat
      841 ctggccgccg acttcttcag aggacttcgg ctcagccgag gcaccccggt gccgcgacgg
      901 gtgggatgaa cccaggaatt cctcacggcc cttggtttgg cctccgagag aatcgttcat
      961 cagtgccacg tcaaaatgct ctacgcgaat ttggtgctca gcgagacgct ctcgcagtcc
     1021 ggcgatgttg tcaagaagga cctcccgcgc cgccagggtt tccacttcaa gacgggccgt
     1081 gagaactccc tcctggactc gcatctgaag tctcagcgca cccaactctg gcggatggag
     1141 tctcaggcga atttcaccat tgcgctcgtg tgccaagcgt atcgcctggg ccacgcgctg
     1201 aacggtgatt atctgctcag cttgatccgc acgcatcgag ggaccttgcg caagagattg
     1261 ttcacccgtc tttggccccg tggaaacctc tgtcctggct aaatcctttc gggtgaagac
     1321 gaaccccgcc ccttcttgtt tcgccccttc gaccgttttt accctttcat cccgctcatt
     1381 ggacactgcc agactggcat tgacgctagg atcgtaggaa gtgacaaggc cggcgttctg
     1441 tggcgtcatc tccgtcgtac cactcgtcaa cttttggctt acggtggggt tcgccccatt
     1501 gtcctgccct gaaagtaccg ccgattcttt cttccgcgaa acggtttgac caataacatc
     1561 tgactgtccc cctgacacag ctacggcagg aaatgcggaa cgaagttgct cggatacggt
     1621 ctcactatcc cccgttgccc gctcctggtt gaccgttccc ccggtttgat tggcttcttc
     1681 gaccgcttcc gaagatggta ccgcgctcgg cattatttga gcctggggcc cacctttatt
     1741 cagctttttc ccgaaaggtt tgacttccga ctcaaaacct aatccggttt cctcaatctg
     1801 atcggtacta ttttcttcgc tcgtaatcaa ctgcaccgtg aggggtaaaa cactctgccc
     1861 tattccgaca acatccgccg tcacatcgct cgctttgtct tgcatgtggg atgtctctgg
     1921 agcggactcc tttacaagag tgtctgcgac gagcgtgcca ggctcggccc cctggccctt
     1981 ttgacagcaa acgactacct ggtagggatt cgtggacgtc tggggctccg cgtcctgccc
     2041 aagcgcggca ccctcgccac tgtttttgcc cgacctctcg acatcgctgt catcgtgcaa
     2101 agcttggtca tgctcttggg cagcaccttc ttttggcatt ttccctgggc aaggctcaat
     2161 aagcttctcg tcgcgagtat cagcaacact ttctccctgg tgggccccgt tttttgtccc
     2221 acccggaatc gtttcagcac atcgagcagt gacactaggg cgcgaggact cttccgtccc
     2281 ctgaatttcc gatgccaaaa cgttccaaaa gctagaaccc acctcctcag cctggtcaga
     2341 gaatcctagg cctcttgtct ggatagcaga caacgaatcg ataatgccta tttcaggagc
     2401 gaacccgatt ctgtcgagca tgatatgttg ccagttcttg tgattgtgtt agttgtaata
     2461 ttccgtattg tgttcagctg tatctaaatc attctgccgc cctcccgatc gcgacctccc
     2521 aagaatccag ttactgctgg gaagttccca attccccgcc ctggcggaga cgcctcaaga
     2581 cctcggcaat cctggccatt tcttcctcgg tacggaactc tgcaatgatg cgggcacgct
     2641 tagagtcgct catgccttgc aaaagcgtga ccacttggtc ctcttccttt tgtgccaaca
     2701 tttcctcgat taattgcttg gcctgagtcg ggcgaaggga ttccaaggtt cgtcgcacgg
     2761 cctccaatcc ggcactttcc gcctcggcct tcatctgggc caagcgttcc tggaatgcct
     2821 tttcctgttg cccaagaacc tcttcttttt tgctcaacgt cgtttcccat tgcagaagct
     2881 catccttgag ggaagcgagc atcttctctt tcagttccag atcgcggtag ctgctcgctc
     2941 gcttggcgag gatatcgcta taagaaggtt cctcggctgg ctctttggga ccgaccggcc
     3001 cggcaatcgc ggtaaccggc ggcggaaggg ttccgcgggc cgcggcgatg gctgtgcgta
     3061 tcttctcggg ggtcaacttc catgcgaacc aaaggtgaat gaaaagacag agtgctccta
     3121 tcgccattgc ggcgaagaag tagagaagca ccgaaagtat ggtctccaag aacctcatca
     3181 tggctcgcgc aagccgtgcg atctgcggtt tgctatttca tccagtttct tcccttcccg
     3241 tgccctttcc tgcttttcat gagcggttcg ttgcttgtcg cgcaaaactt ccaaaatgcg
     3301 gacctcccgg ttggcctcca tcagtgccag ttgccgtttt tcaatctctc cctcgatcgc
     3361 cgacagttgt ttggtcaagt attcgcgctg ggcattcaat acggcctcat accgtcgcca
     3421 agcaagcatt tgatcggcgt caatactgcc ttgttgcaaa ctttcccgaa gtgccgctcc
     3481 caaatgctgg cgctccgcct ccagtgcatc ccgctgccgc agaaggatgt ctcgcgccgc
     3541 atatgcctgt tctagttctc gtttcctttc atccctttcc tgttcacgga tacgtagtac
     3601 agtttccagt ctgaaggtaa atcggcgcat ggaggtttgg ataccattta gtgtccttgt
     3661 ttattgatca aagtttccga ttcatgaacg caccggtggt ccattgaccc cagcgtgccg
     3721 tccggtggac tccttcccgg actgttgcgt cgtagcggcc cgcaaggtct cctgaatttg
     3781 ttcacccagt ttgatcacgc tgtctctggt ttcattgaat tccgcccgct gacccacgtt
     3841 ttgtcgaagg aaagcgttga tcgtatccct tagttggatg gaaagatcga ccttcggatg
     3901 gctgccccga cggtaggctc caattgtgat caaatcttcg tgttccctgt aaacacccat
     3961 caggtccctc acctgcaggg cggactgata atgtcgaggt tcaacaacgt cgggcattag
     4021 ccggctaaga ctctccaaga catcgatggc tggataatgc cctcggttgg ccaagctccg
     4081 cgagagccaa atatgtccgt cgagtaatcc gcgtgccgcg tcagccaccg gttcgtcagg
     4141 gtcattacct tccaccaaca cggtgtagaa cgccgtgatg cttccccggg gtgttcgtcc
     4201 tgcccgctca acaagtcgcg ggagaagggc aaaaacactg ggcggataac cgcgtgtcgt
     4261 agggggttcg ccggcggcca gtccgatttc cctctgggcc agggcgaatc gcgtaaggga
     4321 gtccatgagc aaaagcacat ccttaccctg atcccgaaaa tactccgcga ttgccgtggc
     4381 tgcgtaggcc gcctggactc gcaaaatggc cggttcgcta ctagtggcaa ccactaccac
     4441 gcttttcttc aggccttccg gccccaaatc cctttcgata aagtcattga cctcccgccc
     4501 ccgctcacca atgagtccga tgacaatgac atcggccgcc gtataacggg ccatcatgcc
     4561 catcaggaca ctcttgccca ccccggctgc tgcaaaaatg ccgagacgct gtccccgccc
     4621 acaagtgaga agaccgtcaa tggctttaat ccccgtggga agcggatcct gaatcctggg
     4681 gcgctcgcac gggtggggtg ccggttgatc aaaaaggaca cggtgcttaa gaaacggctg
     4741 agccttgccg tcgatcacgt ggccctgggc gttcaccacc cggccgagca agtcattccc
     4801 cacgcgtaac caatgcagtg tcctggccaa ccgtacccga caccccggcc gcacaccttc
     4861 tggacgaccc aaaggaaata aaattgtcga gtcgttcttg aatccgatga cctccgccaa
     4921 cacgttaccg ccatgaggaa tctcgatttc cgcaagagac cccactggga ccgaaaagcc
     4981 gtgggcgatc atggtgatgc cgaggctttg cgtcaccgat ccttcgatct ctgcggaaat
     5041 gattcgctct aaatggctct taaggtcaat cattttcttg gcacgcagtc ttccccaatg
     5101 ttttcattgg cctgtcaatt cctcgatgat ccgttgcacc tgtttctcga actgttggtc
     5161 tatggagcca aagtcggtct caacccgaca tcccccccgc gtgatcgcgg gatcaggcaa
     5221 gacctcgccc acagccgccg gggataattc ctgaaggatt tttgtcacaa cagggcgaag
     5281 agccttttcg tcttctgggt gcaatcggat tcgcacccgc ggggccttgg ccgccagctc
     5341 gagcgtctcg cgaatcaacg ggacggcaac ttcaggggtt tcaatcacag cttgccgcac
     5401 gagcttaacg gcgatggccc ccgccaagcg taaaagcgat ttttcccact ccgccagaaa
     5461 tgcctgcctc gcctggttga tctgtgatac ggcctcccgc aaggcaggga gaaccgtggt
     5521 aaggcttttc tccaattcac tgcgaataac ccgttctgct tgctccgtcc ctgatcgtcg
     5581 tccttcgatc tcggcgcgtt tacgaatttc ggctgcttct ttttgggcct gggcaacgat
     5641 ctggacggcc tgtttccgca cgccgtccag gtatgccctg gcgcgctcgg ccatgtcttc
     5701 aaaattgaac ggcgtcgcct gggcgactcc tgcaccatcg tgaaccttga tgactgagga
     5761 ccgagcttcc attggcatcc caaccaaaac tcacttcgct atcagatgcc ttttctccca
     5821 caagacacag tctgcatcag agcccgtaag tactccccgg cgaacgtgcc gacccggtag
     5881 ccaccggatg cgattgcaat tccgggggca gtcggatgcg gcgacaagcc gccaacttgc
     5941 tggcgtattc tgcaaggcgg cggcgggcct cctcaacatc actcagtcgg accggtccca
     6001 aatcggccag ctcccggcga atggcctcag ccctccacgt cgggaactgc tggagcactt
     6061 tttccacaag gcccaaggga gccccccaaa gcgagaggac aaccaactcg tcatcagcct
     6121 tttcaaaaat cgtcgccagg gccgattcat cgagacggat gaactggttg aaatcgatct
     6181 ttggaatcgg cgagagtcgc tccgccaaat cgggagcaaa gtttgcgaga ttatccagaa
     6241 tctgctgact aacatgctcc tgagattgat gcacaatccc attgaccgca gtgatcccgg
     6301 caatccgtcg tcgttgaatg gccaccaact gagatagctt ggactccaag gcacgatcga
     6361 cttctcgaag gacctcagga tctgtttctt cgaggctgat caagcacctg atgatgtcca
     6421 cctgcatcgc gggaggaaac cggaccagca cggccccggc ctggtgtggc ggcaagtgcg
     6481 ccaagaccaa ggcaatcgtc tgagggcgtt caccctgaag aatccccacg agtttatcgg
     6541 cctcggcctc ccgcaatagc cgaaagggtg gtgatccctc agtgtcttcg ttcttctttg
     6601 ccggtgcatt cattgacgac tggagtatca attctgtgcc ttcccactgg ggaggcgtgc
     6661 tggttaacgt tgtttcgcgc gatggttggg ccgttgtttg catgaactct cgaatgacct
     6721 gttcccgctc ttcagccggg attcgtccga gggccatcca agtacttcgg acttgatccg
     6781 cgtactcccg cggaaggcga tccagtacta agtccgcggt cttggcgtct aggcaatcga
     6841 taaaaatcgc agctttgcgc aatccagcgt gggccatatc ttgctttcag gtcctctgat
     6901 taaatgggat tgccgatcca cgtcttgaga atgttggcag ccgtttcggg atcatcggcc
     6961 accagctctg ccacttcgtc gcggagtgat tttcccgtct ccgatgttcg ccacttcttc
     7021 ttttctgact tcttttgttc ctcttgagct gctgtttcct ctgccgctgc ggctaccgcg
     7081 ggagccaccc ctgccggagc cgagggggtt gcgcgcaccg ccgaccgcaa gataatcaaa
     7141 ctaaagacca ccaggcccat cagacccagc gttggccagt aaacccggag aaagtcaaac
     7201 gtttgctcca caatgctgac tggctgtggc ggcggtaatg gcatatcgac aaaatcagtg
     7261 acagtgacca attgagaggg gtcctgtacg ttttcgacga cgggcaacag ggccatgacg
     7321 tggtttctga ttttggtcat ctcttccgtt cgtattttgt cgagctcctg tggcgtcggc
     7381 ccctgtgggt tgttggcttg tgtcggctgt gattgctccc agatacggcg gaagtaactg
     7441 gccgggatgg caacagaaac ggtcacccgc ttgggaagaa ggcccgccgt ctcctgctga
     7501 atcaccgtct gtccaacggt gttcttttcc gtccgttggg tttcttcttc tgtttcgctc
     7561 ggtgttgggg tattgcctcc actgagagat cgtggtgtgt ttccttgggc gacgtagccc
     7621 acacgtcctc ctgggtcgct tgtggattgc cgggtctttg tagtttccgt cacgtccaac
     7681 ggaacggcct ttgggtcgat tttgacctgc tcctggcgtt ggtactttgt gggatcaagt
     7741 tcgacattac aacttaccag gactccgggg acgtatccca aggcggtttt gaccttttcc
     7801 gcaaagtagg tctcccacga acgtttcaaa gcgtaggtcc cctcagcagc ttggagaggt
     7861 tcgtcgttgg tcacatagta gttggcccct gtgtccatat tggtgacgac aacctcttcg
     7921 ggtttgatgc caccgatact cttggcgacg taataacgaa tggatcgcac gacttgttcc
     7981 gaaagctcct gccccccgat cggcttaatg gcgacactcg ccgtggtgat ggtttctcgc
     8041 cgtaaaccgc cttggttaag tcgtgagaac attacaacgg cattctgcac gcctgccatt
     8101 tggctgataa cccgcgagag ttccttttgc agggcgatct tcgtccgctc ctccagctta
     8161 tagcggcttt cgaaggcact ggactcattg attgtctttt ccagaatatc caggtagtta
     8221 taaggcaggg catttccttc agccaaggct gccacgtatt tatcttgctt tcccagggga
     8281 acgcggattc tggtgccctc gattcgatag tcgtttagac ccttggcggc aaacgcagcc
     8341 tccatagctg ggagatcggt tggcgagaag tgggcgccgt tcatgagata ggtcgatcca
     8401 ccgcttccgc tgtaggtgaa aagatacccg agactcacaa ggacaacgac gagaagtagg
     8461 acggtcgtca cccttgttcc cggcgtcagg gaaaggaaga catctttcag ttgatcccag
     8521 gtcttattga tgaagtccat ccttggacat ccatatcact aatcgtcgtt aaccgagtgg
     8581 tatcacttca ctaatggcac tctcattcct ctttgccacg aatttcagac ccggatgttc
     8641 tggagttcct gataagcctg caccagttta tttcgaatct gcatcattag cttgaaggcg
     8701 atgtcggcct tctgcacggc agttaagacc tccgcaggcg tgacatcctc ccccgtgatg
     8761 agacgctcca ctgcccggtc ggcctcctgt tgcatggagt tgacctcatt gatcgagcgg
     8821 aggaggaagt ccttgaactc acccgactga gttacccgac cgggtccagt cggcccaaca
     8881 ggagtgacac tacttggact gatgggacga atcaggttca tcaggccaga atcctcaacg
     8941 tctgctgcgt caggtcttta gtgatctcaa tggtgccaag attggcttcg taggctcggg
     9001 cagcctcaag ggcgtctgtc atctccgtca tcatgttgat atctggatag gcgacgtagc
     9061 ccttcatcgg ccctgactgg atcgcatccg ggtgtcccgg ctcatacttg tagcgcggtg
     9121 ggcgatcact ttcaacgcgc actgctgcca ccttgacccc gctggcccca tattgtccga
     9181 tgctggtgtc ggcttgaaag accacgtacc ttggtttgta cggttccggt tggcccagct
     9241 cattgtgtgt ggtgctgatg ttggcaatat tcccggcaat ggcggtcatg cgtgtccgct
     9301 gcgcaaccag cgcgctcgta ctgatgtcca atgccgaaaa catatctcac ctcatcagta
     9361 gatattcaag ccaatggcgt actcaccttg cctgagcgta cgccaggcta ggcgatgacc
     9421 acctctaaaa cgatatgggg tcgaatcatt acacatgttc actgattgcc gtcaagagta
     9481 aacgaaactg gttggtcata atcgtgatcg ccgtattgtg caaaatctgg ttcttgacca
     9541 tctcactcac ttgttgttcc agcgaaatgt cattcccgtc gtgccgaagg ataggccgag
     9601 cctccttgct ccactggggc gaactggccc ttcgaagata gacggcatct cccggtgaca
     9661 tcgacggccc ccgtccatgc cgggcggcca ccgcctcttt caaataaccc tgaaaatctt
     9721 ggaccggtaa atcgacggcc tggtagcctg gggtatccag attcgccaga ttaccggcca
     9781 gtacggtatg gcgaacctgg gagaaatcgg ccaccgccga taaaaggggg atcgtcgttt
     9841 gcgtaaacag tccgttccac atgaccaatg tttctcagct ctaggcttag gagtgcataa
     9901 gcaactataa tgccaaacct cgcggaccag gacttctggt cttctgttgt cacaaagtgg
     9961 tgcaaatcat gcagtttgga aagtatctcg cggcgacctt ttgcatgtta tcctgctcgc
    10021 ggtttgtcac accggcgaga attgccgaat gactccgcaa aaattgccga tcatcttcac
    10081 ccggcgcggg aaaaacgttt ttcgcggggt gcgtagccat attgccggag tttctctgtc
    10141 agggttcgca caccaatgcc caaggccttc gccgttttct cgcgatgtcc cccaaatcgc
    10201 tcaagggtgg cttcaattag gtaccgttcc atctcctgga tactcattcc tacggggata
    10261 ttttcgaact tcggcctcgc ggttacattg ggtagccact tgcgaagctg gtcggcacgt
    10321 actgttgtct ctgtggtcaa gacgcaaatt cgtgtaataa gattctctag ttcccgcaca
    10381 ttgcccggcc aatcgtactc cataagtaaa gctagagccg agtcgtcgag ctcaggcacg
    10441 ggtcgggcca gccgttgggc gcactgttgc tggaaatggg taataagttc cggaatatcc
    10501 tcctttcgtt gacgcagggg cggaacccaa atgggtagca cggctaggcg gtaatacaga
    10561 tcttgccgaa atcgcccttc cgcaatttcc ttttccaagt cacgattcgt ggtagcaaga
    10621 acacgcacat cgacatggag cgtacggctg gaaccgaccc gctcaaaggc tttttcctga
    10681 aggacccgca gcagcttggc ctggaggggc ggctcgatct cggtcacttc gtcaagcagc
    10741 aacgagccac cgttggccat ctcgaacctt cccacccggt cgcaatctgc acccgtaaac
    10801 gcgcccttct cgtggccaaa cagttcgctt tccataaggt gcgccgaaag cgccggacaa
    10861 ttgacactga caaacggtcc cgctgcccgt gaactctgaa catgaatggc ccgcgccacc
    10921 agttcctttc ccgtgccgct ttcaccgtag atgaggacgg tctcactcgt gccggcaacc
    10981 tgggcgatgc gctgtcgcag ctccgtcatc agggaactgg atcccaccaa gttcacggct
    11041 ttgattgtgt ggtcccccgt tctgttggct tgcccagcga gtcgaagtct gctgtggcgt
    11101 actgcttctg caaccaggcg ctccagttga ttggcatcaa atggtttttc gatatagtcg
    11161 aaggcgccgt ggcgtatggc ctcaacagcg gaggaaacgg tggcatacgc cgtgaccatg
    11221 atgggacggg tccgcagttg ttgtttctcc acgtagcgga ccagatccag ccccgacatg
    11281 cccggcatat taaggtctgt caccaccacg tcatagtcag cgcgattgag ggcactcatc
    11341 gcctcggctg cactggaaca catttccaca tgatggccgg cttgccgaag aatatcggcg
    11401 attgcctgcc gagacggtgt gtggtcatcc accaccaaca ccgttcccgg ctgggtgtcg
    11461 tatttgcgat aattcagttc agttcgtttg tctcgtgtca tgggcctctc tattaaactc
    11521 ccggaaatgc ttcaaagtta tccggaaacg atgatcctaa catccctcct gctggtggcc
    11581 ggacaatacg gctcgctggg gtaacacgac cacaaattgt gcgccgcctt ctgggttatt
    11641 gcttgcttgc acgtgtccgc catgaagttc cgtcaaacat tccacaatgg ccaatcctag
    11701 acccgtacca ttgggcttcg tcgtatagaa aggttcaaaa attcggtgca ggtgctcctc
    11761 gtccaacccc gaacccgagt cgctgaccct cagccacaag tcaccctctt ctgcatgagc
    11821 ctcgatcgtg agggtccccc cgtggggcat ggcgtcgagg gcgttgagca ccagattgag
    11881 gaggacccgg cgcattagct cgcgatctgc ccacacatag agggcttgag aaacctccac
    11941 gaccgttttg atctgctgtg cctggatttg aggctcaagc ccagcaagca cctcttcgac
    12001 gagccctagc aagggaaata gaacactctg cggttctcga tccgctgcaa actgaagtaa
    12061 gtcggtcact gtggtttctg ccgcttgaac cgcctcaatg actttgtgga ggatctcgcg
    12121 ggatccctca tcctcagtcc ggcgctccaa aaggctcaga tatagggcca cggggaccaa
    12181 actgttgcga agctcgtggg caatgtgggc cgccatacga cctaggtctg ccaagcggtc
    12241 cttccgcgcc aattcacgat ttttgcgctc cagttccgcg gtcaatcggc ggacttctgc
    12301 ctgaagggcc tcgtgggttt gctcaaggcg gatcgtggcc gcatgccatg cctcaaggat
    12361 cgtgcccaga tcagtttcgg cacttagttc caaatccgtt ttctgattca cagaagatcc
    12421 tccgatgatc gtttgccgat tgttaccact tccgtttggt tttcgaacaa ctctgcccga
    12481 tggattcaca atgtgagatc cagttgcccc gactcatcac cgggttgctg atatgccgtg
    12541 cgggcccgca caaagtcatg aagctcggcc aactgtttgg caatgtcctg cttgcgttgt
    12601 tccagaatgt gggcgcattc actttctgtg cgaaggattt cctccaataa ggcccggctt
    12661 tcaatcagca caccggcaat ggcttctcgc cattcctgtg gaatggcgtc aacttcgtct
    12721 ccataggcgc caatttcttt aaggcgatga tcaatggtct gcaaatcccc gaggacacgc
    12781 tgcttgaccg caagtatctc aaggagaccc tcgatatcgc ccgatgtgac gcaccccatc
    12841 tggcgctgac ccagctcgtg tacccgtttc aaacaccggt gcttctgaac aatgagatcc
    12901 gctagggact cataaacatt cgcagagtga ggcataaagt tcacacgttc tgtaggcctt
    12961 tgatcaatgt cttctccgta aattcattcc tgctttcaac ggcttccgct gctcaactgc
    13021 caggagcaac ttagagccta ttccataatc tcccctcgcc cgttcggcgg gagaggggac
    13081 tttttgagaa agcctctcaa ggttgacttt tgaactgatc atggtgtttg caacagtttg
    13141 tccagacgcg tggcccattc cgcgcgtgaa aaaggagttg ttcgttcaaa tggaatctca
    13201 ttgggaaaac gagtgaggac gattttcgcc tgttccaggg caattcgtgc atctggattc
    13261 tgtcccatag ctcgacgaca gcgagcgatc tcgacatagg catccaggac ctccggctcc
    13321 gcttgaaatc gattggtaat ggtcgtgaag gccgccacgg cctcagcgaa ttgacctagg
    13381 tgataacaca ccctaccgat agcgaagtac gtgttgcgga gcattttgcg ggtgtcttcg
    13441 cttggctcct ccgttgtcca ttgaaagtcc aaatcactct ggacttgccg atagtagtgg
    13501 agcgaccgca agaaatcgtt gcggatcgtg gccatgtcgg gagtggcagc gctactggag
    13561 ggagacagca tcattcgatt ccatgccagt cgagagtaaa tctcagcaag gtaatacttg
    13621 gccttggtgc tgcgggggtc ctcagggtac cgccgaattg cttcctccaa gcgggggatc
    13681 gcgtcctggt cacgcccgag ctgatagtac atctcaccaa gggtgaataa cgaatctcgc
    13741 cattcgacac tcgtgggtgc cagctcacca gaaaggtttg ccaggagcat ctcctcggct
    13801 ttatcgagcg accccttttc catatgtgcg tgtgcggcca aaagtcgcgc agaataggag
    13861 gccgcatctt ttcgatgaaa ttcgatacac tcattaagga cgagtagtgc ttcatcgacc
    13921 cgccccaaag ccagtaggca ctcgcccagt cgcaacaagg caattgggtt acggcgccgg
    13981 gcctcgttgc gaagatactc tcggtaaacc ctcgatgccc gtgagtaact ccccccacga
    14041 tagtaatgct cggcggcctg ccacagatcc tctgtgtaaa accgcgtccc acttcgagtg
    14101 cgtgccagtc gtgtgaactc gtccccggcc agccgaaaac atcgtttggc ccacgttatg
    14161 ttgatttgcc ggtcggacga ttcgctttga gcttgtcgga ggagcgtctc accccacgct
    14221 gataagacgg tggcccgcaa ctcgtgcacc tcggcctcgg cgaacgactt ctccaaggag
    14281 tcgatcacgt tcatcgcaat ctcgtagagt tgcttctcct tgtaggttgc aagggcatct
    14341 ctcacgaggg tctgggcttc agaccacttt acccacgggt taaagaattc ctgtgaatca
    14401 acgatctccc taaaacaaga gatgaaggct tcactggcct gctggtaccg tgacgtctcc
    14461 tggtataact gggccaggcg caggcgggcg gcccaagctt cgtaagtccc cggatactcg
    14521 gagtgacatc cttcccaagc cgcaatggcc ttgtcgtatt caccaaggcc ctcgaaaacc
    14581 agccctaaca aataagtagc cctcgagcgg agcgactcgt cggcatcgac caacctccgc
    14641 acgcgctcaa gcctctccgc agccgaccgc aatctgtcct ggccagcttt gttttcttcc
    14701 tggcttgtaa gaagcctctt tcctaactcg tagtcgatcc aaccgcgaag aaattgggcc
    14761 tcaggtgatt gcgccgcttg tgggctgagc ttcgtcagcg acgacgtggc atcatccgga
    14821 tttcccaggg caatctgaat cttcgcccgc tgcatccacc cggcattctt tgcttgcaca
    14881 tcaagacgcg aaatttgcaa gaatgcctca ttgaacttaa gggcttcctc cttaattcct
    14941 tgcggatcta gtagatgaga ctcggcgaga agccaataga ctcgggcctg cttatccacc
    15001 gtgcgtttac cagcctcgcg cagccatttt cggctttctg caaatcgggc gcggtaatga
    15061 aggcaccgtc ccaaaagaaa tgccgcctcc tcttcccacc cgcgggtaaa gccaaccgat
    15121 gccgcctgct ctccataatt aatggccaca tccaagaggc gacgtcggtc ttcgccctta
    15181 gccgcgtcgg cctctctcgc tacggcaaaa aaccgtgctg acagggccac ccgtagttga
    15241 tcaggcgaca cgtcctcagt ggcaaggact ttatcagtaa gctctcggac caaggggtaa
    15301 tcaccccttt tcaaagccga aaggatttgc tcggcctggg agttatctgg tgaacctcgc
    15361 agaaaaaacc acagaccaac ccccgctccg accgcaatca aggttagccc aatccccact
    15421 gccagggcca agcgaaactt ggacttaacc acgctcgttg ccatggccac aagccgagtc
    15481 cgcagtgata ccctgccgcc ctgttcaacg ggggcggcct gggcactttc ggcagctgtg
    15541 ttgggggagg gttcaggttc ggacatgggt catgaattgt cgtctcctac aaaggcttcc
    15601 gccgaccaag taaaggctat cgcttccaca gatcactacc gtcccagaca caaacgctgc
    15661 attctttgca ggtttacccg cactgatggc atatatccgg ggagaaagta tgtacagaat
    15721 gaaccgtcgc ccgtcaacat caaacggtaa gacgtgtcaa tctgctgaag actgaaaaag
    15781 aaggcaattt cggcgattcg gccaacgaaa tggcagatgt gtcaccgcca cacaatgcga
    15841 caagatggcg actttaagga atctagctac ttttgcaggt cctcagtggg gtaccaccga
    15901 ccgagcgcat agccatgtcg gcgggcctat tccgatttgc gtcaaccgcc tctcagaggc
    15961 tcaggcattc cccaaaaggg taaatcgata catcttggcg aatgctttaa aacacctagc
    16021 gtcgccgcgg tcaccacaaa agagaacggg atgcggaccg gcgtaggctt cgcaaacgtc
    16081 ggcaaccccc tccagttcga ccagaactcg cgtggcgcac ccgccggttg gggggcatgt
    16141 tggcgaactg atgacttttc cccgataaca cattaacgtg tttcggcccg tcaaatattt
    16201 tgtgagaaga accggttcgc ccggcgacca ctggacgtcc aaggccgggg ttttcggaag
    16261 ttgatggaaa aacgggcgga caaaaaactc ctgcgatggg ccctgtggcc catgaagttt
    16321 ccacgcacag gtgcagtgtg ccccgaaata gagattttca ctggtgtcga actcgggatt
    16381 atgctggaaa ccgcctttgt caaggagcca tcgccccagc atgagggtga gcgtggcatt
    16441 gatatcgttt tcacagccgc agggcaccag ttgcccattg tgccaggcaa aactgtaaca
    16501 aggcttgcgg tgcttcagca tcaggcattg aatggtgatg gcatcagcgc cataccgacg
    16561 catgatgtgc tgcacagcgt gatgggcacg gatcgcgtcc accatcgctt cctctgaaac
    16621 gtccacaact ttgcttgcca ttcgccgaaa ggcagcggct tcctcctgaa gttgtggagt
    16681 tggagaaatc gagtcaaaca gatcgttgaa ctcagcagcc gggattgcca cgatggccgt
    16741 accgagactg ggctcctggc tttcggtcaa ctccttgaca gccccgctaa cgcgaaggac
    16801 tctgctttga gccagcattt tcttcgcgcg aacagcccgc aatcctcgct caatctcggc
    16861 aaagttttcc atggaatgga tgtaatagag accttccgcc ttgcgaagag attccggcgg
    16921 aagctggtga tttgatccca aggaatgata cacgattgcg gttggcgcag cctccgtagc
    16981 aattcgtgct gccgcccagg acagcgtatt ccaaaaattg ataaccagca cggcgtcaag
    17041 ctccgcggca cgggcctggg caatgaactc gtccaccttg gccttctggt atagggcctc
    17101 gggatgaatg acgagatcga tacccaaatc gctggccatt ttttgaatga cccccgtgaa
    17161 cttagcctgc tgcgcttccg gctcaaactg attccccggc caagtgaacc attggtgtgg
    17221 ggcccaagag tcctccatct tgcccgcaag cacaacatcg gccggcggat ataggaacac
    17281 cgcccagatc cgcggcggtt tcttttcgat gggtggaaga acgacttcct gagccaccgc
    17341 cgaagaatcc tcagaggcac gtgcgaccaa cgtgttgacc attgctgaaa cccctgcgag
    17401 gcccgccatc tggagaacat cgcgtcgggg aaaacgaccc aacccacaac tttcacgcat
    17461 agattgacct cgcatcgatc ccagcaatcc cgcttttccc cacaccttca atcggccaga
    17521 actcccccaa taaaacgtgc tatcatcata ccgtgggggt tgggggcaaa caaccttcga
    17581 cctcagcaaa gcgactggaa caccagggaa ccgtttgcga tgtgacttcc tttcacctca
    17641 tacggcttcg ctacctgagt ttgccgtgat cacggcggta tgtgaccctt tataatgtcg
    17701 ggccggcaca agccctaccg ctaaggacgg cgggagaacg cacagtcgat cagcaaatac
    17761 cgcatctcac gatgcgcgct catggcattt tgagtcggtc actgcatgtc gtactgggtg
    17821 gttcttgata caatagggcg gtgaactggg agcggcgccc cgttgcggct caccgcattt
    17881 gcccagattc aagaatcttt gggttagtct cctgtggaag tacccgattg gttgaccgtt
    17941 ctgtgtgtgg ggaggatttg atgatgagtc ggagcattct tcggatcgcc gcctgcttcc
    18001 ttgctgttgt tgtggcggtt ttcaatgaac atttgcttgc tgatcaacaa ccggagcgac
    18061 ccaacattgt atggttaaca agtgaggaca atgctatctt ctggtaccgc ctttacaacc
    18121 ctgacggggc gccgatgccg aatgtcgaaa ggctagcgaa ggaaggcctc gtgttcaata
    18181 actgttattc ctgtgcgccc gtttgctcgc cggcaaggag cacgatcatt tcgggctgtt
    18241 atgtcccccg gcttggtgcc cactggcatc ggaaaatcct tcccgtcacg ctccctgcgg
    18301 ggcttcacat gtttccgtgg taccttcgcc aagccggtta ctacaccacg aacaaccata
    18361 aagaggatta caattttgct gccagtgaga aagccgatgt ttgggatgaa tcatctcccc
    18421 gtgcgacata ccgcaatcgc aagcctggtc agccattttt ccatgtacag aactatacga
    18481 ccacgcacga aagccaactt tttccagagc ggcccaacga tcaactgatc actgatccca
    18541 agtcggtaaa gcttttccct tatcatccgg acacacctat cttccggaac aaatatgcac
    18601 attattttga tcttcaccgg caattggatg cacaaattgg gacattcatc agccaactcg
    18661 aggcggatgg tctgctcgac gacacgttca tttttcactt tggagacaac gggggtgtcc
    18721 ttcccggtag caaacaatac gcccgagagg acggactcca cgtggccctt gtcgtctatg
    18781 tgccacgaaa atggcagcac ctggtcccgg cgccgcgagg ctcgcggatc aacggagtca
    18841 tcgagttcgt tgacttggcc cctacggtgt tgaacctagc cggtattccc atcccgccgc
    18901 aaatggacgg gcgtcccttc cttgggccag gcgtgacgct ggaggaactg gatcaacgag
    18961 acacggcctt cggtcatgcc gaccgctttg acgagaaata cgacatggtg cgattcctcc
    19021 gtaaggggag attcacctat tggcggagtt atcagccgtt caatttcgac ggcctctata
    19081 acgaatatcg atacaaacag gcggcttacc gcgaatggcg cgatctttac gagaagggcc
    19141 aagtcaacga cagacaaaag gccttctttg agcctcgccc acctgagtgt cttttcgatt
    19201 tggaaaagga cccgcacgaa acccacaact tggcaggtga tcccgcctac gccaatgtat
    19261 tagtgacgat gcggcggcta atgcaagagc gtgtgaaaag cctgcccgat ctcagcttta
    19321 tacccgaacc catctttatc aaagaaagcg gcggagacgg gtttggatac gccagcaagc
    19381 ataaggctcg gattgccagg ttggtcgata ttgccgatct gcaactattg ccgttcgagg
    19441 aggcttatcc atctttgcag caagccttgg cgtcggacga tcccttggag cgctattggg
    19501 gcatcatcac gtgcagcgct tttggcaaaa aggcaatgcc ccttgcagaa aaaatccgcc
    19561 ggctcgctga gtccgacgaa tgctcgtttg tgcgtactcg cgctgcggaa tttctgggat
    19621 tgaccggcct aggtgaccct ggccctatcc tcatcgacgt tctgaagaag attgacgatc
    19681 cagccggagc aaacctcatc ctcaacacgg ttgttcttct tcgggatggc gcacccaggc
    19741 tgcaaatcga cctgtcgccg ttgcgtaatg cttcttggtt taccgcgtcg ggtgaggttt
    19801 cgaggcgtgt caactatctc tttggccagg gccaataaga ccatcatttg tgtttcttcc
    19861 tggactttaa acgctgcgag attgtcgatt cataaggtcg ccgaaagact tgtcggtgca
    19921 cacagttcga ttgaaacgcg tgtcggcttg gtaaccgtag gaccaaccgc gaaagacagc
    19981 tcaggggagc gatgacatga caggttttac atcttggaat cgtcgggcgt tcttaaggca
    20041 attcggcgca ctctgctgtg gtggcatcgg actcagctta aacgtccgag gcgaaacatc
    20101 gggagaggaa gcccgcctgg cgtttcgccc tgagggcgac ggatttgcct ttgacacagg
    20161 attgctccgc ggaacactgc gtcagcaagg gcgccctatc ggaatggcgc ctctttacta
    20221 caaagatttt tcctccgatt tgagccagcc ctatggtgtg tgttcgcatt atcggctgct
    20281 tgatgcggaa acccgttacg gtgtcgccgc ttgggattgg tcggggacgg cagaactcct
    20341 ctccgacgga agcgtcaagg ccctgtggac acgtgacgaa actcatccct ttgacttaca
    20401 ggcgcactac cgctggcatg ccccagatgt gcttgaagtc cttacgactg tcgtcgcagc
    20461 caaacctttg caaaagttcg aggttttctt ggcatcctac tttaacggct ttgcagacag
    20521 cttcgtctgg gcacggtcct ccccggcggc aagtggggag gctaccttta tgaaagccga
    20581 gcagaagaac ggcgtttggc agatgtttcc gcgggatgag ttggctatta aactcattca
    20641 ggatggtcga tggtcgcgtc ctccgcatcc cgtgaactgg gccatcatgc cattcttcgc
    20701 agccccgttg gccatgcggc gcgatgccga aacaggcttg gtcgccgttg ttatgtcccg
    20761 cccggaagat tgcttcgcag ttgccacgcc ctattccggt gagggccatc ggtccattta
    20821 cttctccctg ttcggccggg atttagaacc cgagcaacct gtcacggcgg aggtccgcct
    20881 gatcatagga catgcgctga ttgacgaagt cattgtggac cgataccgcc aatatgctgg
    20941 tggagaaaaa cgaaacgcct agccccctgg catcagcatg caccaccaag gaatcgccct
    21001 cacgatgcaa gtttcgatcg gtcacgcgta aggccgaatc atcctcgatc ggtgctatgg
    21061 cagcggggga cgtcccactt gctccgacaa tttccgcaca gtttctacaa cgactttctc
    21121 ggcttctggg gcgaaaaagc caagtgtgcc aacgccgcgg gtttcgtagc cgccttctcg
    21181 gatgaccttt gcggaaggca gatatccgaa cacctcattg cagtagcccg ctatccaaag
    21241 ccgaacatgc ccgatttcgt tttcgatcag cggaacaaag tcggagacaa cctcacccga
    21301 tatggccacc aatgtcaagt cgttgccaaa ctgccagaca gcgatcgggg ccttataatg
    21361 cgacggcgga gattcgccgc gctggagcgt ctctatcatt cgcttcgccg tttcgctcag
    21421 atgggaaggc cccttggcca tgctctccaa cttctcgcgg tcgggcgggg gaaggagtgg
    21481 aagctcgacc tctgcgaggg ccgtcttcag aggcccggat acaggactca gcttttcccc
    21541 aaccacccgg cacacctctg ttcccaactc ctggccatgc tgcttggcgt gctcgatttc
    21601 gccccgcgga tacgggttcg catcgccgcc gcagccaatc atgaaaaggg ccatcacgcc
    21661 cggcagtttt tcctcgataa aggccttggc aaatccggcg taatcacctg agatgacgta
    21721 atgctggccc gtaagcgtcg tattatggca tgcacagccg aaaagcacgg cacgcaactg
    21781 atccttctca tccgtaatct ttaggacggg aacacttcga tcggcataac ctctgggatt
    21841 gacacccagt cggacaccgg tttccgtgaa ttcacggcga ttcatgggga aatgcacgac
    21901 gccaacgccc cacgcaagtc gggccggctt caaatcggcg atggcctcag cggccaggtc
    21961 agcatactgg gcaatcagcc cgcgggtata tgtttccaca accttttggt cttcctcacc
    22021 catgctgtac ccggccgggc gcgacaaacc gatcaccggt cctgtatgcg tatgcgacca
    22081 gttgaaaagg atctgctctc gtgccaggcc cgtcttctcc atgatcgcac gcgccgctgc
    22141 ctcggcaacc tctttgcgaa agccgatgac atcggctgtg attagcacgc cgcggttgcc
    22201 ttgactatcc tcaatcgcca gcgccgttgc gtagagcggc gcgaggaccc cttcggaggg
    22261 cttgttgcgc gaggcgtaac cagccaacca aatcggctgt tgaggcgtga tatccttcct
    22321 cgccaagcct atcttccacg gtaaaccgct gtcttctgcg gcaacacagc aggaacttaa
    22381 ccccacgagc agcattgcga ctgccaaaga catggtcacg ccacgcaaaa aggtagccat
    22441 cgcagcacct cctcaggcaa aacccaccac ccaacatcca aaaagcaacg caacttgcta
    22501 atcatcttat ctcaaaaagg ttcctcaaac catcagcaac cgcggccaac gatgggttgg
    22561 agaagctccg cacaacctag ccggatcatc ggtacacaaa aacagccaac aagccaggag
    22621 gagcaacccg ggcggcttgg caatcgtcgt cattcggtcc cgatatagtt gatcatggcg
    22681 attgaatcac ctaaagtgat ggcatccttc ggaacatccc acaggacgtg cgaagccaca
    22741 tgtttgctac tttagactcg ccacgggagg agatgacaat gagttgccac cggttcgatc
    22801 atgcagtgcg cggattgcgg ctattggtta tcgccacggt cgttctgggc acggttccct
    22861 ggcaagccat gggaatggaa ttggccaaga caggcacccg acgtaccgtc gtctcaattg
    22921 atggaacgcg gtggctgatc aatgggcagg tcacatatcc aggcagtccg gccgaagggc
    22981 tcctcatgaa tgtgcggatg gtcaacgcaa cattcgagga cttaaatcgc caagattttg
    23041 accccgaagc aaatacggag cgttttttag cccatttgcc cgactacgta gcccatggaa
    23101 tacgtgcgat cacactcaat ttccagggtg gacacgcagg ttatgaagga ccgcttaact
    23161 cggcgtttaa ccctgatggg tcgttgagag aggagccact taagcggatc aaacgggtta
    23221 tcgaagcctg tgatcagcaa ggcgtggtcg tcatcttggg ctgtttttat cagcgacagg
    23281 atcagattct caaagatgaa gaggctgtca aaacggcctt ggtcaacgcc gtccgctgga
    23341 tcgagcagca agacttcacc aatgtggtcc tcgaagtggc gaacgagttc ccccatcggg
    23401 gttttgacca cgctatcctg agaaagcccg aaggccaagt ggagctaatt cggctggcca
    23461 aaaagacaag ccctcgccta ctggtttcca ccagtggtat cggcgatggt cggtttcctg
    23521 acgtggtggc cgaggtggcc gactttatcc tgattcattt caatggtgtc cctgtcgagg
    23581 agatcccgga acggattgcc gcgctcaagc gatttggaaa gccaatcgtt tgtaacgaag
    23641 acgacaagac aggaagcgtc gcagctcaag ccgcagagct atgcgtcaag aatggtgctt
    23701 catggggact gatgcttagc aagctgaatc aatattggcc ctttgagttc catagtgcgg
    23761 ccgatgatcc ggtggtctat gcaaaactca aggaattgac aacagccggt gatcgaaaca
    23821 agtaggcgaa ccctggtgtt gagcgattgt ttcggtaaaa acctcctgtt tgtgtgtcgc
    23881 tttgagcatc cgatggacat caacgcaagg agatttgtgc catgacgaga accattgtct
    23941 ggcaaaagaa aacgatgcgg ccgacccact tgctgcatgt gctggttctg ggatttattg
    24001 tcccatgggc caatgtttat ggggaacagg caagcccgcc gagccttatt gtggctgttc
    24061 gcagctatcc agaacccggc aagatcgtgg cctccgtggt cgtgcctgag gttgccgata
    24121 ttgccgagaa cgccgaattg actctccagg tcactctgct tgcacccgat gagaaagtct
    24181 tactccagag ccaccaaacc acccaagtgg ggatgcgaga ggaagtcatc ctggatgccg
    24241 cccacatagc gcctggcgag tacaagatca tggccgtgct cgttgggccg ggttcgcagg
    24301 agttactgcg atcgcaacat ccggtgaagt ggcccggacg aagtgaattg ttccgcggca
    24361 tccgcattct caacaatctt gtttgggaac tattgaacgt cgaggcaaca ggcgatgcac
    24421 ccatatcggg cgaatacgtg gtgcagcttc cttacgatcg ctggttgttt gtgcgcctga
    24481 ccctccagga cggacaagac gacgaggtga aagttagcct ccgcgacagc cgtggtgtgt
    24541 taccccttgt gacacatccg ggctttttac ccgggacgat cgaggcgatg cgttttgcaa
    24601 aggcaggacg ttacgtgatt gaagttacgg cgaacaaggg aaaggtgaaa cagattgtgg
    24661 ttcgcgccgt gccggagatt caacatagcc gctatcccac gagtgtgcca tacatcgatc
    24721 aaggtgttcg ctacgactgg gagtatttgg aacgccacgt cttgccgcat gtcaacacga
    24781 tcatcacgtc gggaccttcc gaagaggaaa tccgccattt gcaggattgg aaagagcgcg
    24841 ggggcaaagt tatcgtctat acaggcaggc cgggtcttca cggcaagaaa gagcttgagg
    24901 cgtcgcccga ggtctgtgcc aactactggg cgagccaggc cggttatacg ctgcctttct
    24961 ccgacggaat cttggtggat gaattctatc acaatgagga cccagcatac ccggcgtaca
    25021 cagaagctgt caggcttctc aatacccgtt ttccaggtaa ggcgttctat ccgtatgcgg
    25081 ctggccggtt tggaaaggac caaggcagta ttccgttcgc caaagtctgc atcgagggtg
    25141 gcggctaccc atgctgggag gcctatatcg cggaatggcc aaccctcaag ctggcgctca
    25201 acaagctacg agaatacccg tacgaacacg tgatcccatt tgaagagaaa cttccgggag
    25261 ttaccaagaa attgatttgg gtctttgggg tgttttcgtt cccctggccg tatgccgatg
    25321 gctatgcgaa cgtcaactat aacgcctacc tggatatgca gtttcagttt atcgccaccc
    25381 atccggcctt attcggtcta ggcggtatgc atatctggcg aagtgggtac tgcgacgagg
    25441 aacgactgcg atggtgtgct cgtctcttcc gtcactacgc attggaagga aacacaaatc
    25501 gcctgagcgc tgatccatac atgctcacac atatcgagaa tcccgacttc gtggaaggtt
    25561 tgaagcattg ggaggtttca cccgcccgcg aaaattccgt ttttgctact gctcaaaagg
    25621 gatttggcaa gtttattggt cgatactacc gtggtccgga cagctttctc gtcacgcggc
    25681 gcgacccgga aaagcccaac gtggttcggc aggttattcg gaacctcgaa ccaggacggc
    25741 tttactcggt aaaaatgttc acaggggact acggggcttt ccaaagtggg aactctactc
    25801 gcgagttaca cgccgtgtcc cttcagatca aaggcgcaga attactttcc ggaccgcagt
    25861 accgttatca acaggccttt ccaacacggg aacgcttggg caacttcact gagcaaaagc
    25921 cgttttggct taatttgcac tggcttgtgt ttcgggcgac ggctcccact gcggagctgc
    25981 tgatctccga ttggaaagcc ccgcagaatc caggtggacc gcaagaccaa acgctgttgt
    26041 acacatacgt cgaggtaaag ccctacctcg actagggtga ccagcgaaat gaatggaacc
    26101 tctgcctgcg taataagaac ctatcccacg acctcccctc tctcgttcag cgggagaggg
    26161 gtcgggggtg agggtggctc aaatcgctgg cgttttctcg atcgactcca atgatcgctt
    26221 gccctcaccc caaccctctc ccagagggag agggaacttt gggataggct ccagatagcc
    26281 gcaccagagt cgcggggcag atcggccgtc ttgagcccga gcccgtccga cgagtgagct
    26341 tcggtcacac gccgtgacca ccctattcct tgacgtgcgg tgccgctgcg tcattttccg
    26401 catcacgtca caaccggcct tggccaagtc cgctacacgt tgttccgggc cattactgcc
    26461 gcgtcttccg ctgaaccccc tctgtcggat tcttgtgcca gcaagaagga tgacactata
    26521 cgtgaccatt cacgcgggac gggtgatcct tctgaaagat gcccgtaatg gccgggtcac
    26581 tatcccgagc cacatcggga gagtcgccgt ccccggcgac caaatcagcg gacggcgagg
    26641 acgccgtccc tccctcctct tcaccgacgc caccaggtcc ttatgatgca gccctctcgc
    26701 gtcccaagaa gtggcgatct gcgctgtgaa cggttacgat gatactgctg aaggcctatg
    26761 cttgcgcaca gattccacgt gtctattgta aactgccgcg tcggtgggct atggtcggga
    26821 gagtcgcatc gtttgggtca cttgaggtcg aacatatgag aactgttatg gttcagattg
    26881 ttcggcatgt cgcgctttgc agtgtcctgc ttttcgctgc taatggcaaa gcgttcggac
    26941 agaatacggc tcagccagtt gtgtcggacg agcagatccc cgatggctct ctcgatcctt
    27001 ttttgggaaa gccggatctt caaaaacaga ttttgttcag cggagagcga tttccgaata
    27061 ttgttgtcac caaaaaaggg acagttctcg ccgtatgggg aagtcagtct gttcgttgtc
    27121 gtcgaagcga ggatggtggg caaacctggg agaaggaaat tgcaatcgca gtcccaggat
    27181 ttcatggggg tggtgtaact gtcgatgaaa caaatggtca catctttgtt tttgttcagg
    27241 ctcagcatcc cccagcacca ttaaccgttt accgaagtcg tgatgacgga aaaacgtggg
    27301 agcaaactcc cgtgacgatt catccggacc agaaaggcca tacgccgtcc atgcacatgg
    27361 ccgagcacgg gatcaccctg tgctatggtc cggccaaagg gaggctaatc cgcccggcaa
    27421 gagtctatga tcgaccacgg ggctataaca ccgccattta cagcgatgac ggcggcgtca
    27481 cgtggcacac aagtgcccct tttcccgatg agggcacagg ggaaggcgcg atcgccgagc
    27541 tgaccgacgg ccgcctgtat tacagctcac gcaagcatca ctttgaaaat attgccgatt
    27601 tctcttggaa gcgttt
//
LOCUS       DAOSUT010000041        27345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1159_length_27345_cov_9.571821, whole genome shotgun sequence.
ACCESSION   DAOSUT010000041 DAOSUT010000000
VERSION     DAOSUT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 27345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27345
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_27345_cov_9.571821"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..2617
                     /locus_tag="PLD05_05460"
     CDS             554..2617
                     /locus_tag="PLD05_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP76916.1"
                     /translation="MRVIRQLMFWVACGFAWGVVTQAVFARSGAGERPNILVLIADDQ
                     RYDALSVVQKEQGERGRFPWFQTPHLDRLAAEGVRFRNAFVVNSLCAPSRAVMLTGRY
                     NHLNGIASNFRPFPLENVTYATILKQAGYVTGYFGKWHMDSQRERPGFDFFASFIGQG
                     VYFNCPLLVQGVETRTEGWVDDVATDYALKFIRETTAEKKPWLVVLGFKSPHGPFTPP
                     ERAKERFTGCLARRTPNFDVRPPYLPEARTQQTSGEASESAEVPVNLDYFRCISGVDD
                     CVGRILAGLEEYGVAEDTLIIFQSDNGFYLGEHGLGDKRSAYDESLRIPLLARYPKAI
                     VKGLVRDEMVLNLDLAPTILDFAGLPIPSQMQGQSWRPLAEGKQVPWRKAWLYEYFAE
                     KQRNSRVPDIVAVRTESAKLITYPGHDDWTELFDLKNDPYETRNLYRDPAAATLRAAM
                     EAELERLKKDVDYRVPDYVDRPDWWGGSPPDPNAPPEVCLDYQLGEAGQGDTIVDRSG
                     RGNNGVNRGTVVRQSSDGQTVRYFDGNGLIEVPNSPTLNPADMSWTVEVVFRPEAADG
                     VLVARGGQSQGYALWLREGRPVWTVVVSNQVISVESAEQVTGWATLTGVITPQRQAAL
                     YVNGTLVGRKPLPDFISTDPRDSMQIGADTGSPIVPAGTQRFKGEMKRVTIYRGARPP
                     QTTGQ"
     gene            complement(2808..4049)
                     /locus_tag="PLD05_05465"
     CDS             complement(2808..4049)
                     /locus_tag="PLD05_05465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP76917.1"
                     /translation="MVTVEKTPTIATPPLAIEGGSPVRKAPFPPWPVFEPDEVTAVVR
                     VLQSGKVNYWTGQEARRFEEEFAQAVECRYAVAVANGTVALEAALEALGIGPGDEVVV
                     TCRSFVASAACVLRVGATPVFADVDRDSQNITAETIAPVLTPRTRAVIAVHLAGWPCD
                     MDPIMELASRAGLAVIEDCAQAHGARYKGQPVGSLGHIAAFSFCQDKIITTGGEGGMV
                     TTNDPQLWRKVWELKDHGKNWDLIRQPGNGTFRWVHESLGTNWRMTEMQAAIGRVALT
                     KLENWVGIRRANAAILRRRLAHLPALRIPSPPPTVEHSYYKFYAFLRPEFLKPGWDRD
                     RILAAIQAEGIPCGAGVCPEIYREKLFQDRALGPAAPLPVAHELGQTSLMFLIHPTLE
                     ERDMIDTATAIEKVLTAATVG"
     gene            complement(4113..5207)
                     /locus_tag="PLD05_05470"
     CDS             complement(4113..5207)
                     /locus_tag="PLD05_05470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP76918.1"
                     /translation="MGHIRLILPFQRQAWYDVLFRCERFDVYHLPEYYLTVSTVEAGK
                     PFLLAYEEENYVAALPVLIRPVDSVPELKGLGWCDATSAYGYPGLLTNCRPDAPKADE
                     FRRRWQDALHQLLRSLHIVALFVREHPLFSATWMWHPMAIVKPLGQTVVLDLQRPEEE
                     QLRAMRQNHRRNIRDAIKCGITVVEDESLERLGEFQRLYEATMLAVNAEPYYFFPQSY
                     YEGLRHHLHGRVKLLFAVLDGEPVAGAIFFHCGNVLQYHLGGSNPALGRERAMGMKLI
                     MDTVRRWGSANGFRWFHLGGGLGARQDSLFHFKAGFSDLRCDYHVVNYVVDQEAYGEL
                     VRRRFGDDSAASLTNGWFPAYRCPTVRRAA"
     gene            complement(5223..6470)
                     /locus_tag="PLD05_05475"
     CDS             complement(5223..6470)
                     /locus_tag="PLD05_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOP76919.1"
                     /translation="MPLRVLFINQHFAVPHQGAGTFPFTVMSELVRRGHEVTVLTTDQ
                     HSSHGRSYVTEENGICVHWACIPYANRMRYWRRIVAFFEFTAWCLAKIRRLAPFDVVY
                     ASSTPLTVALPGVYASRRWAVPFVFEVRDLWPEVPIAMGALRNPVSIAAARWLERFAY
                     RNAAFVIARSPMMADGVVETGYPRERVRVVPNACDFRRFDVPAEAGRSFRQENQWLAD
                     RPLVLYAGTFGAVNDCSYIVRLAAEVEPLLPEARFLLVGQGREENLIRNLAQELGVLD
                     RTLFIHPPVPKSEVPRLFSAANLTMSVTADIPALHKNCANKFFDSLAAGRAIAINHEG
                     WLADLIREHDCGLVLPARDIPAAARMLAEALRNRQWLREAGRRAYQLGRKQFDAIRCI
                     DEIEAVLYAAVGDHRITYRRAAA"
     gene            complement(6764..7999)
                     /locus_tag="PLD05_05480"
     CDS             complement(6764..7999)
                     /locus_tag="PLD05_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP76920.1"
                     /translation="MRLLILSSVIAVTICLVGRGGIDGKSLYADTVTLHGGGRIVGQI
                     INSPEGQTDKTVTIQTEDQAIISIPKSEVKEIISLSEVQAKYQRLRQQTPDTVEGHWK
                     LAEWCRDHNLSKEREVHLRRIIELDPEHQRARALLGYQKVGDQWKTRDEIMKSMGYVL
                     YKGRWMLPQQVQLEQEREQQTAVQRDWSLRIHRLIKGLETDAVTAEREIKAIRDPAAV
                     KPIVLAMRNESREAVRLLFTDVLGQIGTPEAARALAIWAIEDPVEEVRLHALEALAPL
                     KSGEIVSYFVGRLRDKDNAIVNRAAQALQRMGDPSAIPALIDALVTRHKIFIPGTRPP
                     TGMAAGFSSSSTGKSGMSFGAGSGGDTPKVVERPFSNPAVLEALVELSGGQNFGYDVR
                     LWKHWYATQKQAEATKAMQ"
     gene            complement(8260..9297)
                     /locus_tag="PLD05_05485"
     CDS             complement(8260..9297)
                     /locus_tag="PLD05_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOP76921.1"
                     /translation="MSNQLSRRSVLGALAAGGFLAHGGFARFGIAEAHQPFTAAQALE
                     KIKAGRKIPVILDTDIGDDIDDTWALVLLLKSRQLDVRLVVGDNRNALYRGKLIAKLL
                     EVAGRTDIPVGLGVGLEDRPGRQSPWIGDYDLKKYPGKVHEDGVQAIIDTIRSSPEPV
                     TLICIGPVQNIAEVLKRAPDVAQNARFVGMHGSVYKGYGGSKEISAEYNVKVDPKALQ
                     AVFAASWEVTITPLDTCGLVHLTGEKFRKVYESSDKLVRAVMDNYQVWRETQAKKQLP
                     PPERSSTLFDTVAIYLAQSEELTEMETLSLTVTDDGFTRIDPNGRPVRAAVRWKDLGA
                     YEDWLVEHLIS"
     gene            9972..11342
                     /locus_tag="PLD05_05490"
     CDS             9972..11342
                     /locus_tag="PLD05_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76922.1"
                     /translation="MSISRRTTRRRFLQGSVGLGVTAIILSQRRYAVGFESANERPVF
                     ATIGLRNQGWEITQKTFKFADFAALADVDAKVLAENVQKTEQRQGKKPDAYKDYRYVL
                     DRKDIDAVMIATPDHWHTKIAVEAMYAGKDVYCEKPLTLTIDEGKLIEKVVKETGRVF
                     QVGTMQRTECDKRFLIAVALVKHGRIGKVRKVTCGINGMEPSPPIPEAPVPEGLDWDF
                     WLGPAEKVPYRALPELRTGYGGGVPYYSNCHYSFRNWHEYSGGKLTDWGAHHVDIAVW
                     AIGATDTGPSKVTPLNYTLACEYKDGYPVVHDRYNVATQFNIRAEMPGDIELIITSEG
                     DNGILFEGTEGRFFVNRGRITGKPVEELQDNPLPEDALEQIYGGPISANHSANFIEAM
                     RARKQPISDVWSHNRMLEICHLANIAMRLGRELRWDPVKREIVGDPQANSFLSRPSRK
                     GYEIKI"
     gene            complement(11512..11667)
                     /locus_tag="PLD05_05495"
     CDS             complement(11512..11667)
                     /locus_tag="PLD05_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76923.1"
                     /translation="MSADVWVIEDDDPNSRGGLARDAVPRVRHSGGTPRPDQTKEIAP
                     PHADAHP"
     gene            complement(11863..12393)
                     /locus_tag="PLD05_05500"
     CDS             complement(11863..12393)
                     /locus_tag="PLD05_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOP76924.1"
                     /translation="MPIYEFYCDKCHVIYNFFSRRINTDKRPDCPKCGRPKLERRVSR
                     FAIGRGGGSENKSGDDAEADDDLLPPGFDEEKFEEAMMELATEAENLDEDDPRQMARL
                     MRRLSQKTGLQFGSAMEEAIRRLEAGEDPETIEEELGDVLEQEEPLFQQSGKSLRTLY
                     EHLKPPRVDEKLYDLD"
     gene            12586..13452
                     /gene="rfbD"
                     /locus_tag="PLD05_05505"
     CDS             12586..13452
                     /gene="rfbD"
                     /locus_tag="PLD05_05505"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOP76925.1"
                     /translation="MPIAVTGVKGQLGGELCRQLGDRAVGLDLPEFDLLDYDRVRKTI
                     RELQPELLIHAAAYTAVDKAEDEPEKCFAINATATGVLVDLARELDCPFLYVSTDYVF
                     GGEPERSEPFREEDVPSPQGVYARSKYEGELKTLVWEKHYIVRTCGLYGQLGPHSAGN
                     FVETMIRLANEGRPLRVVDDQFCTPSYVVHVARAIIFLIQTDMYGTFHIVNDGATTWY
                     RFAREIFRQLKMDVDITPISTEEYGAKAPRPRFSVLDTSKYNRFFGAPPLPHWKEALA
                     EFLARRAEFLGH"
     gene            13584..16118
                     /locus_tag="PLD05_05510"
     CDS             13584..16118
                     /locus_tag="PLD05_05510"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76926.1"
                     /translation="MTNHVWPKMMSFCALGLTSGISKRRSMLKAVTLVGYISVGMFAF
                     LLSGQVLAATVEEDQKPWKPGPLLQKMLEGPLANVEEIVFAVRVPGRDHWYVTFGNYS
                     DYSETPAKALGFKFEDGVYWGYGEGARLCALNLRTGRIRILLEDSKGGIRDPQVHYDG
                     QKILFSYRKGGTHPFHLYEINVDGTGLRQLTDGPDDDIEPTYCPDGSIIFCSSRCHRF
                     VNCWYTRVAILYRCDADGKNIRPLSSNNDHDNTPWMLPDGRVLYMRWEYVDRSQVHFH
                     HLWTMNPDGTFQTVFFGNMYGGVAMLDAKPIPNTNKVVVSWSPGHGRPEHLGPVAVVD
                     PSLGPDRQDAVKILGKERNWKDPYAISEDCFLVAHPRGLFVMDGEGTAELIYQLSPEE
                     QKTFQCHEPRPIVPRPRERIIPPRVDLTKATGTLVLEDIYRGRNMEGVPRGAVKKLLV
                     LKQLPKPVNFSGGMEPLTIGGSFTLAEIVGEVPVEPDGSANFEVPALQSLFFVALDEN
                     DIAIKRMHSFVTLQPGEVTSCVGCHEVRTHAPHAVSTDLLALRHPPHQIQPISDVPKV
                     LDFPRDIQPILDRHCVACHQPDRREGKIDLTGDHTARYSISYWTIRVHNLVSDGRNEP
                     RGNRPPYSYGSAASRLMKLIDGSHYGAKLSPLEVKTIRLWIETSACYPGTYAALGCGF
                     YPVQLPWGELGQRCGNCHMGEIQTAEGPKKSLIFRGGFPGATEPLSNLDCPEKSYILL
                     APLAKAAGGLELCGKPIFADTQDPLYQKTLAAIQDAHRRLQEGKRFDMPGFRPNQHYI
                     REMQRFGFIPKDLGPNDPIDYYKVDRDYWDSFLYQPMKAGTLVTGE"
     gene            complement(16161..17024)
                     /locus_tag="PLD05_05515"
     CDS             complement(16161..17024)
                     /locus_tag="PLD05_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76927.1"
                     /translation="MKDILGPKKPGRSLHAEISVDGQDWQSVITLAKNTIVTLCTTTL
                     KCGDRQLRVTSPWREVCRFDDRQVSHREYERRLSGGIRHQRQVTLCPRDDFFLIAETF
                     LASRGTTWEYQLEIGLSRNTAVHPLPETQELELAVGDYAGAVLPLAVPEWKSLTPQPG
                     LVFEGDHIVARYATTGRAFFFPLCFLFWKDKRAAGNPPLAESSANGQPLPVTWRQLTV
                     SECLRKVSADEAVGFRFQLEKRQWLLYRSLTPPANRAVLGHNLVSEFLLARFTPKGQV
                     IPLVEVELDEE"
     gene            17368..17838
                     /locus_tag="PLD05_05520"
     CDS             17368..17838
                     /locus_tag="PLD05_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76928.1"
                     /translation="MNTDRADGRFAVIGDITRDHLAGLSIRRKPYQGTTEPAVARSLD
                     EQGEIMLRRIVLATLLAVLIGTFFQQVASAQQAYGRQWGRTYSTQDWQRFYHYPYVYY
                     PQNYWGPDYYRSSEDLYYRYPPEMRIPVYNRNWQNFYPEPRRYYQGHHFIMDIF"
     gene            complement(17987..19024)
                     /locus_tag="PLD05_05525"
     CDS             complement(17987..19024)
                     /locus_tag="PLD05_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOP76929.1"
                     /translation="MQRRHFLETASALICSIPFGFSVSFAEETSPKTEWPDAFTQNKR
                     LGRGVNIIGYDPLWRDRSRARFREEFFPLIRQAGFQHVRINLHPFRDNPQGGEGAGLR
                     PEYLETMDWAVDHALKAGLLVVLDFHEFTAIGRNPDKLKDRYMACWQVIAERQKNRPP
                     EVLFELLNEPNSAMTPELWNQWSREALALVRRRNPKRTVIIGPGQWNNIAALDKLELP
                     ADDPNIIVTVHYYSPFDFTHQGASWAGKADKVGIHWGSEEEKAAVRADFDRAQAWAER
                     HKRPLYLGEFGVYDKAPQEDRVTWLQFVTREAERRGWSWAYWQFDGDFILYDIPKNQW
                     VEHILKAIIPE"
     gene            complement(19117..20544)
                     /locus_tag="PLD05_05530"
     CDS             complement(19117..20544)
                     /locus_tag="PLD05_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOP76930.1"
                     /translation="MGNPTGFLQYPRVEVDHRPVAERIRDWHEIDRPLAERVLNEQAA
                     RCMDCGIPFCHMAGCPVKNRIPEFNDLVYRGRWREAAQNLHSTNNFPEITGRVCPAPC
                     EPACTLSINSQPVTIKHIEYQIAEKAFAEKWVRPLKPTHRTGKRVAIVGSGPAGLAAA
                     QQLCRLGHEVVVFERSDRIGGLLRYGIPDFKLEKHVIDRRLEQMVAEGVHFEPNVWVG
                     RDITADQLRRDFHAVLLAMGAGKPRPLTVPGADAKGVHFAMDYLTQQNRRVAGDPIPT
                     SGPNSIYARDKHVVVIGGGDTGSDCVGTAIRQGAKSVLQIEILPKPPEGKNPETPWPL
                     WPRIMRTSTSHEEGCTRRWSVLTKALTVHNGQVTHLHGCEVEWVRGPRGWEMREKPNT
                     DFTVPADLVLLAMGFLHVEHDGLIEQFGLELDNRGNVRVTRWMTSVPGVFAAGDTVKG
                     ASLVVHAINHGREAARAVDKYLQSL"
     gene            complement(20537..25108)
                     /gene="gltB"
                     /locus_tag="PLD05_05535"
     CDS             complement(20537..25108)
                     /gene="gltB"
                     /locus_tag="PLD05_05535"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOP76931.1"
                     /translation="MNQQNRRRPPQAYGLYDPQYEHDACGIGAVVNISGKADHRIIDL
                     GKQILLNLMHRGASVADETTGDGAGILFQIPHDFFLEEMASDKIDLPAPGEYGVAMVF
                     FPREDEVRLRCETLLESEVVTSGLTLIGRREVPTDNSCLGEIARACEPRIFQFFIHGN
                     GLRGEELERRLFITRKRLEHRVRGELGEAAADFYIPSMSSLTIVYKGMFIAPQLAAYY
                     PDLSDSRLKSALAIVHQRYSTNTFPSWKLAQPFRMIAHNGEINTLRGNVNHLAAYEKK
                     MTSPLLGQDLSRLCPIIEPGTSDSGAFDNCMELLVRAGRSAPHALMMMIPEAFGPRYH
                     ISTDKRAFYEYHAAIMEPWDGPAAIVFTDGRLVGGTLDRNGLRPCRYVVTDDGLVILA
                     SETGVIEFPPEKIRQKGRLQPGKMFLVDTKEQRIISDNEVKGKIIRQRPYRRWLEQNR
                     IELRGLFLPPTDVESEKGPLEQRLRAFGYTVEDLNMILAPMARDGQEPVGSMGNDAAL
                     PVLSDKPKLLFSYFKQLFAQVTNPPIDPFREGLVMSLMSFTGKHRNLLDETPEHCRQL
                     KLPHPILTNDDIRRLREANHPHLKVATVSILMPAKCENPGATLRETLEKIVDSAERVI
                     RDGASLIILSDRGVNAEWVPIPSLLATSALHHGLLRRGLRGEAGIIVESGEPREVMHF
                     CLLCGYGANAINPYMAFEAIAALHRNKDLPPNFSLDEAIEQYITAVKKGILKTMSKMG
                     ISTLRSYHGAQQFEAVGLDRSVVDAYFCGTASRIGGIDLQVIAEEALARHRDAFGPRE
                     PAALPLDFGGEYHERVDGERHLWNAETVSKLQHAVINNDWETYLQFAERVNCQNEELC
                     TLRGLFEFEPGEPVPLEEVEPASSIVRRFYTGAMSHGSISREAHEIMAIAMNRLGALS
                     NTGEGGEDPARYRPLPNGDSLNCGIKQVASARFGVTIEYLANAKILQIKMAQGAKPGE
                     GGQLPGHKVTEEIARLRHAMPGVSLISPPPHHDIYSIEDLAQLIYDLKCANPEAMVSV
                     KLVAEVGVGTVAAGVAKGNADEILISGHDGGTGASPWSSIKHAGIPWEIGLAETQQTL
                     VLNGLRDRVLVQVDGQMRTGRDVVIGALLGAERFGFGTAALVSLGCVLMRKCHLGTCP
                     VGIATQMPELRARFAGKAEYLIRYLLFVAEEVRRWMAQLGFRRFDDMVGRVDRLRVRR
                     AIDHWKAKGLDFSAVLAPPPVPEGTPLRRVRPQTDKHQDHLDRVLLPQVQAAIDNMQP
                     MRLEMPIRNIHRTVGTTLSYHIVKKHGSRGLPDGTIHLVFHGSAGQSFGAFLAPGITL
                     ELIGDANDYLGKGLSGGRIVVRTPPGSPFDPAENVIVGNTVLYGATSGEVFINGLAGE
                     RFAVRNSGATAVVEGVGDHGCEYMTGGVVVVLGKTGRNFAAGMSGGIAYVLDEYQLFD
                     TLCNLDLVELESVWQPQDQQQLRQLIERHYAWTGSEQAKRLLDKWPESVGKFVKVIPI
                     EYRKALERIREREQVRAEQLPATEEVFSG"
     gene            complement(25458..26807)
                     /locus_tag="PLD05_05540"
     CDS             complement(25458..26807)
                     /locus_tag="PLD05_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP76932.1"
                     /translation="MIKQKNFFDGFSASLCRINARFLVILVLFGPEMISRADNWPAWR
                     GPEGTGVSRETNLPLSWSEEANQGIVWKTAIPEWGNSTPVIWENALFITTQAEDRLLV
                     LRLDKTTGKIVWTRQVGTAETPENKPRGEQKFHRLNNNASPSVVTDGEHVVAHFGNGD
                     LAVFTFDGELLWKRNLQADYGKYTIWWGHANSPVIVDGLVVSVCMQDSLADLQDKPHP
                     SYVVAHDLKTGKEVWYTPRMTGAMRESCDAYTTPLVRRLPDRTEIVVMGAEQLDAYDA
                     RTGKQIWYLPGLTGNRTITGPTIENDIVFATIGMRGATFAAKIGGEGRLPPERILWRY
                     EGNNPDSPSPVYSHGLLFLAADNGIVQCLDAKTGELKWRERLGREFKASPLAAEDRIY
                     FLDTSGRCTVVRAAPTFEVLSVNEVKDRSIASPAVSDGHIFIRGRQFVYCIGRPFTR"
     gene            complement(26879..27337)
                     /gene="ndk"
                     /locus_tag="PLD05_05545"
     CDS             complement(26879..27337)
                     /gene="ndk"
                     /locus_tag="PLD05_05545"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOP76933.1"
                     /translation="MERTLVLLKPDCVQRRLMGRIISRFEDKGLNIVAMKMIRVTPEL
                     ARKHYAEHVHKAWYPSLEEFITSGPIVAMAVEGPEAIRVVREMVGATNALAAAPGTIR
                     GDFGCSRQMNLVHASDGPESAARELAIFFNNEELCPYTPTIQSWLRAKDE"
BASE COUNT         5861 a         7814 c         7734 g         5936 t
ORIGIN      
        1 tgtgggcggg gaggaccgga tcgatgaagt ctcaccaccg gtgaatacca cgcttgttgt
       61 cgggatcggc tggctgccgc tgtgagcggt gtgtcgttcc cacgttctct cacaaccgct
      121 tgaccatgct gcctcccagc gggtgctagc cttacggcca ctggtagttc taattcgtta
      181 ctactctttg cggctggttt tggcatccac caagtccgcg tcatttttga aagcggttgc
      241 cagcgtttgg cctcgtcgcg gccttctatc gacgaggggt tgtaggtctt gtgacgactt
      301 tgggcctact tgctgaaaaa gaccaaggtc gtcagaacat gtcccctcgt accgaaggcg
      361 cgtgactcct ctgagccttg ctggatagca ctgcagatgg gatatacacg ccatgttggt
      421 ggtactagtc taggtcccga atgttttcag gttagactag gaggtgcttt tggcattact
      481 cgggcaatta gaagtgaaag caccgggtct tgcttgagag gcgaaggcac tttcttgagg
      541 gaggacatag gcgatgcgcg tcattcggca gttgatgttt tgggttgcgt gcggatttgc
      601 ctggggggtt gtgacccagg cggtttttgc tcgaagtgga gctggggagc gtcccaacat
      661 ccttgttctg attgcggatg accagcgcta cgatgcgcta agtgtggtcc agaaggagca
      721 gggagaacgt gggcgatttc cttggtttca aacacctcac ctcgatcgtc tagcggcaga
      781 aggcgtccgt tttcgcaatg cctttgttgt gaattcgctt tgtgcgccct ctcgggcggt
      841 gatgttgaca ggtcggtaca accacctgaa cgggattgcc tctaactttc ggccatttcc
      901 cttggagaac gtgacctacg ccacgatcct caagcaagcg gggtatgtca cgggttattt
      961 tggcaaatgg cacatggata gccaacgaga gcgtcccggt tttgatttct ttgccagttt
     1021 tatcggtcag ggcgtgtact tcaattgccc actgctcgta cagggtgtgg aaacgcggac
     1081 cgagggctgg gttgatgacg tggccaccga ttatgccctc aaattcatcc gtgaaacaac
     1141 ggccgagaaa aaaccgtggc tcgtcgttct cgggtttaaa tcccctcatg gtccgttcac
     1201 gccaccggaa cgggcaaagg agcggtttac cggctgtttg gccagacgaa caccgaattt
     1261 cgatgtccgg cctccctacc ttccggaagc aagaacccag cagaccagcg gtgaggcctc
     1321 ggaatcggcg gaggtgccag ttaatctcga ctatttccgg tgtatcagcg gggttgatga
     1381 ctgtgtcggg cggattctgg ctggtttgga agaatacggg gtggcggagg acacgttgat
     1441 catcttccag agcgacaacg ggttttatct gggcgaacat ggcctcggag acaagcgaag
     1501 cgcgtacgac gaaagcctgc gcatccccct cttggcccgc taccccaaag ccattgtgaa
     1561 gggcctagtg cgggatgaaa tggtcctgaa ccttgacctg gcgcccacga ttctggattt
     1621 tgctggcctc cccatcccca gtcagatgca gggacaaagc tggcgcccac tcgcggaggg
     1681 gaaacaggtg ccgtggcgaa aggcatggct ctacgaatat tttgcggaga aacagcgaaa
     1741 tagccgggtt cccgacatcg ttgccgttcg aacagagtcg gccaagctga ttacctatcc
     1801 gggtcacgac gactggacgg agctgtttga cctgaaaaac gatccctatg aaacccgaaa
     1861 tctttaccgc gacccggctg ccgccacatt gcgggcggcc atggaagcgg agttagagcg
     1921 acttaagaag gacgttgact atcgggttcc cgattacgtc gatcgtcccg attggtgggg
     1981 tggttcaccg ccggacccca atgccccgcc ggaagtttgc ctggattacc aactgggtga
     2041 agccggccaa ggcgatacaa ttgtcgatcg ttcaggccgg ggcaataacg gggttaaccg
     2101 tggcacggtt gtccgtcagt cgagcgacgg tcaaaccgtg cgatatttcg atgggaacgg
     2161 cctgatcgag gtgcccaact cgccaacgct aaacccggcc gatatgtcgt ggacggtcga
     2221 ggtggtcttc cgtccggaag cggcggatgg ggtgctcgtt gcccgaggcg gtcagtccca
     2281 gggctacgcc ctttggttgc gcgaagggcg accggtctgg accgtggttg tcagcaatca
     2341 agttatctcg gtggagtccg cggaacaagt taccggttgg gcgacgctga ccggggtgat
     2401 cacaccacag cgacaggcgg cgctttatgt caacggaaca ttagtaggga gaaagccgct
     2461 gcccgatttc atctcgaccg atccccgcga cagcatgcag attggggcgg atacgggcag
     2521 cccgatcgtt cccgccggaa cccagcgatt caagggagag atgaaacgtg tgacgattta
     2581 tcggggggca cgcccgccac aaacaaccgg ccagtaagga tcggcattga gagcctattc
     2641 caaaaagtcc ccatctgcct ccgggagagg gttagggtga gggcaaggga tcgtcggagt
     2701 cgagcgagaa aaccggcagc gattcgagtc accctcaccc ccgatccctc tcccgccaaa
     2761 cgggcgaggg gaggttgtgg gataggctct aaactaatcc agcgtggtta cccgacggtg
     2821 gccgcggtta gaactttttc gatggcggtc gctgtgtcga tcatgtcgcg ttcttccagt
     2881 gtgggatgaa tgaggaacat caggctggtt tggcccagct cgtgggccac aggaagcggc
     2941 gcggcggggc caagggcgcg gtcttggaag agcttttcgc ggtagatttc tgggcaaaca
     3001 ccggccccac aagggatgcc ctcggcttga attgcggcca ggatgcgatc acgatcccag
     3061 cctggcttga ggaattcagg cctaaggaag gcgtaaaact tgtaatagct gtgctcaacg
     3121 gtgggcggtg ggctgggaat ccgcagcgcc ggcagatggg caaggcggcg ccgaagtatt
     3181 gcggcgttgg cacgacggat accgacccag ttttccagct tggtcaaggc cacccggccg
     3241 atggcggcct gcatctcggt cattcgccaa ttcgtgccta aagactcgtg aacccagcga
     3301 aatgtcccat tccccggctg tcgtatcaaa tcccagtttt tgccatgatc tttgagctcc
     3361 catactttgc gccacagttg gggatcattt gtggtgacca tgccgccttc gcccccggtg
     3421 gtgatgattt tgtcctggca aaatgagaag gccgcaatgt ggcccagtga gccaacgggc
     3481 tggcccttat aacgcgcccc atgcgcctgg gcgcaatcct cgatgacggc cagtcccgcg
     3541 cgggaggcca attccataat ggggtccata tcgcatggcc aacctgctag gtgaacggca
     3601 atgaccgcgc gggtccgggg cgtgagaacg ggggcaatcg tttcggccgt gatgttctga
     3661 ctgtcgcgat ccacgtccgc aaaaacgggc gtggcaccca cccggagcac gcaagcggca
     3721 gaggccacaa aactccggca ggtgacgacc acttcgtctc ccggaccgat ccccaaggcc
     3781 tccagggcgg cctccagggc gaccgtgccg ttggccaccg ccacggcata gcggcattcc
     3841 acagcttggg caaactcctc ttcaaaacgg cgggcttctt gccctgtcca gtagttcact
     3901 ttcccggact gtaatactcg caccacagca gtgacctcat ctggctcgaa gaccggccac
     3961 ggcgggaacg gagccttccg aacaggggaa ccaccttcga tggctagtgg aggtgtggca
     4021 attgttggcg tcttttccac agttaccata ttgtggaatc tcctctcttt gatttgtgat
     4081 tgaggggtaa gcgtggcggt ttcgcgcaag actcatgctg cccgtcttac ggttggacac
     4141 cgatacgcag gaaaccaccc gttggtcaag gaggcggccg aatcgtcacc gaagcggcga
     4201 cggaccaact cgccgtacgc ctcttgatcg acgacgtaat taacgacgtg atagtcacat
     4261 cgcaggtccg aaaaaccggc tttgaagtga aatagtgaat cctgccgtgc tccgaggcct
     4321 ccccccagat ggaaccagcg gaagccgttg gcactacccc agcggcggac ggtatccatg
     4381 atcagtttca tgcccatcgc ccgctcacgt ccgagcgccg gattgcttcc accgagatga
     4441 tactgaagca cattgccaca gtggaagaag atcgcgccag ccacgggttc gccgtcaaga
     4501 accgcaaaga gcagcttcac ccggccatgg aggtgatgcc gcaagccctc atagtacgac
     4561 tgcggaaaga aatagtacgg ttcggcattg accgccaaca tcgtagcctc gtagagccgt
     4621 tggaactcgc caagccgttc cagcgactca tcttcgacga ctgttatgcc acatttgatg
     4681 gcgtcgcgga tgtttctgcg gtgattttga cgcatcgccc taagttgctc ctcttcgggg
     4741 cgctgcaaat cgaggacgac cgtttgtccc aagggtttga cgatggccat cggatgccac
     4801 atccacgttg ccgaaaagag cggatgttct ctgacaaaaa gggcgacaat gtggaggctg
     4861 cgcaaaagct ggtgaagcgc gtcttgccac ctgcgtcgaa actcatcggc ttttggggca
     4921 tcgggccggc aattagtcag taggccggga taaccatacg ccgatgtcgc atcgcaccag
     4981 cctagtcctt tcaattcagg gaccgaatcg acaggccgaa tcaaaacggg cagcgcggcg
     5041 acgtaattct cttcctcata ggcaaggagg aaaggttttc cagcctctac cgtgctaaca
     5101 gtcaagtaat actccggcag gtgatacaca tcgaaccgtt cgcagcggaa gaggacatcg
     5161 taccaggctt ggcgttgaaa ggggagaatc agtcggatgt gtcccatctc tggtgcctct
     5221 gtttaggctg ccgctcgacg gtatgtgatc ctgtgatcgc cgacggcggc atacaaaaca
     5281 gcttcgattt catcgataca acggatggcg tcaaactgtt ttcggccgag ttggtacgcc
     5341 cgtcgtccgg cctcccgcag ccattgccgg ttgcggaggg cctcggccag catccgcgcg
     5401 gcagcgggga tatctcgcgc aggcaagacc aaaccacagt catgctctcg gataaggtct
     5461 gccaaccagc cttcgtggtt gatggcaatg gcccggcctg cggccagact gtcgaaaaac
     5521 ttgttggcac aattcttatg aagggccgga atatctgcgg tgaccgacat ggtcagattg
     5581 gcggcggaaa acagccgcgg gacctcactt ttgggaaccg gcggatggat aaaaagggtt
     5641 cggtcaagta cgcctagctc ttgggcaagg ttgcgaatga ggttctcctc ccgaccttgc
     5701 cccacgagca ggaaacgcgc ctccggtagg aggggttcga cctcggccgc cagccgcacg
     5761 atgtaggagc aatcgttgac ggccccaaaa gtgccggcat aaagcaccaa cgggcggtcg
     5821 gccaaccact ggttctcttg acggaacgaa cgacccgcct cggcagggac gtcaaaacga
     5881 cgaaaatcgc aggcgttcgg tacgacgcgg acccgctcgc gcgggtaacc tgtttccaca
     5941 acaccgtccg ccatcatggg cgaacgcgcg atgacgaagg cggcgtttcg ataagcgaac
     6001 cgctcgagcc agcgagcggc ggcgatgctc accggattgc gtaacgcgcc catcgcaatg
     6061 ggaacctcgg gccaaaggtc gcggacctca aaaacgaagg ggacagccca tcgtcgtgag
     6121 gcgtaaaccc caggcagcgc cacagttagg ggcgtactgg acgcatagac gacatcgaaa
     6181 ggtgctaatc gccttatctt cgccaggcac caggcggtga actcgaaaaa ggccacaatc
     6241 cgccgccaat atctcatgcg attcgcatac ggaatgcacg cccagtgaac gcagataccg
     6301 ttctcttcgg tgacatagct ccggccatgg gacgaatgct gatccgttgt taataccgtg
     6361 acctcatgtc cgcggcgaac aagttctgac atcaccgtaa agggaaaagt gcccgccccc
     6421 tggtgcggca cggcgaaatg ctggtttata aataacacgc gtaaaggcat tgcttgactc
     6481 cgacgacctt gatcgtccgg aagacacacc tggcccacaa tcgttggaag acaaaaatgc
     6541 catcccgccg cgcgtggtag ccagggtctt gcgcaggcgg tgatcatatc agagccagcg
     6601 gaaacggggc aaccccattt ctcaatgggg taagcgcgca ggtgggtagc gaaactgtgg
     6661 gagaaggtta agcgacaaac agctccatcg gccggcccgc gtcggcactt gcgtcaccgc
     6721 gaaaaggcac gccttgtcac agtttctgtg ataccgccga tttttactgc atggccttgg
     6781 ttgcctccgc ctgcttctgc gtggcgtacc aatgcttcca caggcggacg tcatacccga
     6841 agttctggcc accgctcagc tcgacgaggg cttctaacac ggcaggattc gaaaaaggtc
     6901 gttccacaac tttaggagta tcgccgccgg aaccagctcc aaacgacatt cccgacttgc
     6961 ccgtgctgga actgctgaat cccgctgcca tgccggtggg gggacgagtg ccggggatga
     7021 aaatcttgtg ccgggtcacg agggcatcga ttaaggcggg aatggccgac gggtccccca
     7081 tgcgctggag agcctgtgcg gcacggttaa cgatggcgtt gtccttgtcg cggagtcgcc
     7141 caacaaaata gctcacaatc tctcctgact tgagaggtgc aagggcctcc aaggcatgga
     7201 gccgaacctc ctcgacggga tcctcaatgg cccaaatcgc tagcgcccgg gcggcctccg
     7261 gcgtaccaat ctgaccgaga acatccgtaa acaagagtcg caccgcctcc cgactttcat
     7321 ttctcatcgc aagaacaatc ggcttgaccg cggccggatc acgaatggcc ttgatttccc
     7381 gctcggcagt taccgcatcc gtttccagcc ccttaatcaa gcgatgaatc cgcaaagacc
     7441 agtcgcgctg gaccgctgtt tgctgctccc gttcctgctc cagttggacc tgctggggga
     7501 gcatccaccg ccccttataa agaacgtacc ccatcgactt catgatctcg tcgcgtgtct
     7561 tccattgatc ccccaccttt tggtagccca atagggccct ggccctttga tgttctgggt
     7621 ccaattcgat gatccgccgc aaatggacct cacgctcttt agaaagattg tggtcgcgac
     7681 accactccgc aagtttccag tgcccttcca cggtgtcagg tgtctgctga cgcagtcgtt
     7741 ggtacttcgc ttgaacctcg cttaaactga tgatttcctt gacttcggat tttgggatcg
     7801 agattatcgc ctgatcctcg gtctggatgg ttaccgtttt gtctgtttgg ccttcaggcg
     7861 aattgatgat ctgccccact atacggccac cgccgtgcag ggtcacggta tccgcgtaaa
     7921 gcgatttgcc gtcaatgccc cctctaccta ccagacaaat agtgacggca attaccgaac
     7981 ttaaaattaa aagcctcata tccggcgcct cgcggcagaa tagacacgtt attcgacggc
     8041 ctttgaccgt ctcttgttcc ttatctcaag tataagccgg tccatgatcg ctggccaatc
     8101 gaaagttcca gcggatgcga cgacgcgcgc cccttcagaa ctgatggaac cccgcgcaca
     8161 gcgttaaata acggggcaat ccaggggttt gcgtctacca atgcacgacg aagtgctggg
     8221 ggaccacgca ctgtcgcggc cgggaacgac tgtgggcgtt taactgatga ggtgttccac
     8281 caaccaatct tcgtaagcgc ccaaatcctt ccaccgcaca gccgcccgaa cgggtcgccc
     8341 gttgggatcg atccgcgtga aaccgtcgtc ggtgacggtc agcgagagtg tttccatctc
     8401 ggtaagttct tcagattgag cgaggtagat agccaccgta tcaaagaggg tactgcttcg
     8461 ctctggaggt ggaagctgct tcttggcctg cgtttcccgc cagacttggt aattgtccat
     8521 aacagcacgg acaagcttgt ccgaggactc atagaccttg cggaattttt cgcccgtcag
     8581 atggaccagc ccacacgtat ccagcggggt gatcgtgact tcccagcttg ccgcaaagac
     8641 ggcttgaagg gccttgggat caactttcac gttgtactca gcggagatct ctttgcttcc
     8701 gccgtagccc ttgtacacgc ttccatgcat cccaacaaag cgggcatttt gagcgacatc
     8761 tggcgctcgc ttgaggacct ccgcaatgtt ttgcaccggt ccaatgcaaa ttagggtcac
     8821 gggttccgga ctgctacgga tggtgtcgat aatggcttga acgccatctt catggacctt
     8881 accgggatat ttctttaaat cgtagtcgcc gatccagggg ctttgccgcc cgggtcgatc
     8941 ctcgaggcca acgcccaagc ctacggggat gtccgttcgc ccggccactt caagcagctt
     9001 agctatgagt ttcccccgat agagagcatt gcgattgtca cccaccacaa gtcgtacatc
     9061 gagttgccgg gatttcaaaa gaaggaccaa cgcccaggtg tcgtcgatgt catccccgat
     9121 atcggtatca aggatcaccg ggattttcct gccggccttg attttttcca aggcctgggc
     9181 agcggtgaac ggttgatgag cttcggcaat cccaaaccgg gcgaatccac cgtgcgcgag
     9241 aaatcccccg gcggccaggg ctcccaagac cgaccgacgc gaaagctgat ttgacatcgt
     9301 tgcgactccc aggatacgga ccaaatctcc cagaaaacct acattctcgg tgttaccaca
     9361 cacgtgaaga aaacatgctg catggatcgg caagtcaata agattatcgc ctggttcatt
     9421 cacgaaaaca actattcgta tttgcccagc gtccgcgcag attccggggg caaccacagg
     9481 gggttgcccg tacgattgat gccattccca agcagaaggg tgattgaaat cacgtggcac
     9541 gggcatcttg cccgtgtcat tgtgcgtcac ggtgttgagc gcttgtctcc aacggggatc
     9601 ggtgttgtcc agtgatttgg tcgccgggga cggcgacccc ccatcgcgaa attgggaggg
     9661 acggcgccct cgccgtccgg gtcgatatac ccgttgacca ggacgaataa gagcccattg
     9721 agaacctgta ggggcaggcc gccgtgcctg ccccaaattt ccacccccgt gcctgcccaa
     9781 atcgcctcca tgaatttgtt ccgagttttt gctacactta cgttaatcgc gggtgcgtca
     9841 gcgcactatg cagggttggg tctatcgtcc ccaccgcacc cacagatagg ttcgcgtttc
     9901 cgttgtcttg catcaactgt tcgggatgtc ccgacaggta acatcgcatc atttcgaaag
     9961 ggatttagct tatgtcgatc agccgtcgca caacacgtcg tcgttttttg cagggaagcg
    10021 tcggactggg agtgacagcg attattcttt cgcagcgtcg ttatgcggtg gggttcgagt
    10081 ccgcgaacga gcggcccgtc ttcgccacga tcggactgcg taatcaaggc tgggaaatca
    10141 ctcagaagac cttcaagttt gccgacttcg cggccttggc ggacgtcgat gccaaggtgc
    10201 tcgccgaaaa cgtgcaaaag actgagcaac ggcagggcaa aaaaccggat gcttacaaag
    10261 actatcgtta cgtgctcgac cgcaaagata tcgacgcggt gatgattgcc acgcccgacc
    10321 actggcacac caagatcgcc gtggaggcga tgtacgccgg gaaggatgtc tattgcgaga
    10381 aaccgttgac cttaacgatc gacgaaggga agctgatcga aaaggttgtg aaagagaccg
    10441 gccgcgtctt ccaggtcggc accatgcagc gaaccgagtg cgacaagcgg ttcctgatcg
    10501 ccgtggcgct cgtaaagcac ggacggatcg gcaaagtccg aaaagtcacc tgcggcatca
    10561 acggtatgga gccctcgccg cccatccccg aggcacctgt gcccgaaggt ctggattggg
    10621 acttctggct cggacctgcc gagaaagtgc cctatcgcgc gctgcccgaa ctgcgcaccg
    10681 gttacggcgg tggcgtgccc tactacagca actgccacta ctcattccgc aattggcacg
    10741 agtactcggg cggtaagctg accgactggg gcgcgcatca tgtggacatc gccgtctggg
    10801 ccatcggcgc gaccgacacc ggccccagca aggttactcc gctgaactac accttggcct
    10861 gcgagtacaa ggacggctat cccgtcgtgc acgatcgtta caatgtagcc acgcaattta
    10921 acattcgggc cgaaatgccc ggcgacatcg aattgatcat cacaagcgaa ggcgacaacg
    10981 gcatcctctt tgaggggact gagggccgtt tctttgtgaa tcgtggccgc attaccggta
    11041 agcccgtcga agagctccaa gataacccct tgcccgagga tgccttggag caaatttacg
    11101 gcggaccgat tagcgccaac cacagcgcca atttcatcga ggccatgcgg gcccgcaaac
    11161 aacccatctc cgatgtgtgg tcgcacaatc gcatgctgga aatctgccac ttggccaaca
    11221 tcgccatgcg actgggccga gagctgcggt gggatcccgt caagcgcgaa attgtgggcg
    11281 atccgcaagc caattctttc ctctcacgtc caagtcggaa aggctatgag atcaagattt
    11341 aacgccctcg agtgaagatg cgcaggcatt cttggctccg gctagcctgc cactttgatc
    11401 tatcgcactt caccgcgccc gcaagctgat tgaagccgtg taggggcgtt gttaacctag
    11461 aaaaaggccc agggcttgac tttttgggca ggggataggc cctgattgtg cctagggatg
    11521 ggcgtccgcg tgcggtggag cgatttcttt tgtctgatcg ggccgagggg ttccgccgga
    11581 atgacggacg cgggggaccg cgtcccgtgc caatccgccc cgggagttgg ggtcatcgtc
    11641 ctcgatgacc cagacatcgg ccgacatcca ggcccattcc cgacgctctc gggcaggcac
    11701 gggggcctgc ccctacgggt tctgaatggg cccctattcg cggtcaacat ccggacggcg
    11761 aaacgccgtc cctcccatga gtcggccatt ctccaaatcg atttacggac cgcaattttt
    11821 tctcgctttg ttgctctccg cgaaaccagt ggggcgtgtt gattagtcca agtcgtagag
    11881 tttttcgtcc acgcggggtg gtttaaggtg ttcgtaaagg gtgcggagcg atttaccgct
    11941 ttgttggaaa agaggctctt cttgctcaag gacatcgccc agctcttcct cgattgtctc
    12001 cgggtcttcc ccagcttcga gcctgcggat agcttcttcc atggcactac cgaattgaag
    12061 acccgtcttt tgggaaaggc gtcgcatcag gcgggccatc tgacgcggat cgtcttcgtc
    12121 caggttttcc gcctcggtgg ccaattccat catggcctct tcgaattttt cttcgtcaaa
    12181 gcccggcggt aacaagtcgt catcggcttc ggcatcgtcc cccgatttgt tctccgagcc
    12241 gcccccgcgt ccaatggcga accgcgagac ccttcgctcc aatttaggcc gaccgcattt
    12301 gggacagtcc ggacgtttgt cggtattgat ccggcgtgaa aaaaagttgt aaataacgtg
    12361 acacttgtca cagtagaact cgtaaatcgg catggtgctc acctccgttc tagcggcctt
    12421 cccgatttgg attaacaccc ccgtttgtca aaaatcgcgt ttttcctttc tcgccttccc
    12481 atttatacta gcatgaatag cctcttaccg gcgagattgt tactagtaga agcgggaatg
    12541 aggacagaag cgactgtgag gactaggtag tttgtagggg atgttatgcc aatcgcagtg
    12601 accggcgtca aaggacaatt gggaggagag ttgtgccgtc aattgggcga ccgagccgtg
    12661 gggctcgatt tgcccgagtt cgatctcctc gattacgatc gtgttcgtaa gacgatccgg
    12721 gaactgcaac ctgagctttt gatccatgcg gcggcctata cggctgtgga taaggccgaa
    12781 gatgagcccg aaaagtgttt tgccatcaac gccacagcca cgggggtttt ggtcgatctg
    12841 gcaagggagt tggactgtcc ctttctctat gtgagcaccg attatgtctt tgggggcgag
    12901 cccgaacgga gtgagccgtt tcgtgaagag gacgtaccct ctccccaggg tgtctatgct
    12961 cgaagtaagt acgaagggga gctaaaaacc cttgtttggg aaaagcacta catcgtgcgg
    13021 acgtgcggtc tttatggcca attggggcca cattcggcgg gcaattttgt ggagacaatg
    13081 attcgcttgg cgaacgaagg ccgtccccta cgggtggtgg atgatcagtt ttgcacccct
    13141 agttacgtgg tccatgttgc ccgtgcaatc attttcttga tccaaaccga tatgtatggg
    13201 acgttccata tcgtcaatga cggggcaacg acgtggtatc gcttcgcccg tgagatcttc
    13261 cgccaactga agatggacgt tgacattaca ccgatcagca cggaggaata cggtgccaag
    13321 gccccgcgac cgcgcttcag tgtattggac acttccaaat acaatcggtt ttttggtgcc
    13381 cctccgttac cccattggaa agaggccctc gccgagttcc tcgcacgtcg tgctgagttt
    13441 ttaggtcatt gaggccgctg tggcgactgc gcttgatttt tactttgccg tccgtcaaac
    13501 tgaacaggcg tttgattgat cctgcctgcg gacctgcctg gtcccggcca aagtcctgca
    13561 cgctattttt ggggagcctt gccatgacga atcacgtctg gcccaagatg atgtcattct
    13621 gtgccctcgg gttgaccagc ggtatctcga agcggcggtc catgctgaag gccgtcaccc
    13681 tggttggata tatttcggtc ggcatgttcg cttttttgct ttccggccag gtgttggcag
    13741 caacggttga agaggaccaa aagccgtgga agccggggcc gcttcttcaa aaaatgcttg
    13801 agggtccctt ggccaacgtg gaagagatcg ttttcgctgt ccgcgttccg ggccgagacc
    13861 attggtacgt gacctttggc aattactcag attattcaga aacaccggcg aaagccctcg
    13921 gctttaagtt cgaagacggt gtttattggg gctatggaga aggcgcccga ctgtgcgctt
    13981 taaacctacg caccggtagg attcgcatat tgctcgagga ttcgaagggg ggtattcgcg
    14041 atccccaagt ccattacgat ggccaaaaga ttctgttctc gtatcgaaag ggggggacgc
    14101 atccttttca cctttatgaa atcaacgttg acgggacggg tctccgccag ttgaccgatg
    14161 ggccagatga tgacatcgag cccacgtatt gtcccgatgg aagtatcatc ttttgttctt
    14221 cgcgttgcca ccgatttgtc aactgttggt acacccgagt ggcgatcctt taccggtgcg
    14281 atgctgacgg aaagaacatc cggccgcttt ccagcaataa cgatcacgac aacactcctt
    14341 ggatgttgcc ggacggtcgt gtcctttata tgcggtggga atatgttgac cgcagccagg
    14401 tccatttcca tcacctgtgg accatgaatc cggatgggac attccaaact gtctttttcg
    14461 gcaacatgta tggcggtgtt gcgatgttgg atgccaaacc gattcccaac accaacaagg
    14521 tggtggtgtc gtggtcaccg ggccacggtc gtcccgaaca cctagggcct gtggctgtgg
    14581 tcgatcccag ccttggaccc gatcgtcagg atgcggtcaa gattctggga aaggaacgca
    14641 actggaaaga cccgtacgcg atcagcgagg actgcttttt ggtggcccat ccccggggat
    14701 tgtttgtcat ggatggcgag ggtacagccg aactgattta tcaattgtcg ccagaggagc
    14761 aaaagacgtt tcagtgtcac gaaccaaggc ccatcgtgcc gcgcccccgg gaaaggatca
    14821 tcccgccgcg cgtggattta accaaggcca cagggacttt ggtgctggag gatatctacc
    14881 gtgggcgaaa catggaaggt gtgccgcgcg gagcggtcaa aaaacttctc gtcctcaagc
    14941 aattgccgaa accggtcaat ttttcgggcg gaatggagcc gttgacgatt ggcgggtctt
    15001 tcaccctggc tgaaattgtg ggtgaagtgc ccgtcgagcc agatgggtca gccaattttg
    15061 aagtccctgc cttgcagtcg cttttctttg tggccttgga cgaaaacgat atcgccatca
    15121 aacggatgca cagctttgtg acgcttcagc caggggaagt gaccagttgt gtgggctgcc
    15181 atgaggtccg aacccacgca cctcacgcgg tcagcactga tttgttggcc ctgcgtcatc
    15241 ccccgcatca gattcaacca atcagcgacg tgccaaaagt cctggatttt ccccgtgata
    15301 tccagcccat tctcgatcgt cactgtgtgg cctgtcacca gcccgatcgc cgagaaggaa
    15361 agatcgacct gacgggtgat cacacggcgc ggtattcgat aagctattgg actatccgcg
    15421 tgcacaatct ggtctcggat ggccgtaatg aaccgcgggg gaatcggcct ccttacagct
    15481 atggcagtgc ggccagccgg ttgatgaagc tcatcgacgg cagccactat ggggcaaaac
    15541 tcagtccttt ggaagtcaag accattcgcc tgtggattga gactagcgca tgctatcccg
    15601 gcacatacgc cgcgttggga tgtggtttct atcccgttca gttgccttgg ggagagctgg
    15661 gacaacgctg cggaaattgc catatggggg aaattcaaac ggcggaaggc cccaaaaagt
    15721 ccttgatctt ccgcggcgga tttcccggtg cgacggaacc gcttagtaat ctcgattgtc
    15781 cggaaaaatc gtacatcctt ttggcaccct tggccaaagc tgctggcgga ttggagcttt
    15841 gcggaaaacc gatcttcgca gatacccagg atccactgta tcaaaaaacc ctggccgcca
    15901 tccaagatgc ccatcggcga ctacaggaag gtaagcgatt cgacatgccg ggcttccgtc
    15961 ccaatcagca ctatatccgg gagatgcaac ggtttgggtt cattcccaaa gaccttggac
    16021 ccaacgatcc gatcgactat tacaaagtcg atcgcgatta ttgggattcg ttcctctatc
    16081 agccgatgaa ggccggcacc ttggtgacgg gggaataatc agcttgtggt cgttccggtt
    16141 tccctgccac tagtagagtc tcattcttca tcgagttcga cctcaaccag ggggatgacc
    16201 tgtcccttgg gcgtaaaccg cgcaaggagg aactcgctaa cgaggttgtg ccccaggacg
    16261 gcccggtttg caggtggcgt caaggagcga tacagcagcc actgacgctt ttcgagttga
    16321 aagcgaaacc ccacggcttc atctgccgag acttttcgaa ggcactcact gaccgtcagt
    16381 tggcgccaag ttaccgggag gggttgaccg ttcgccgagc tttccgccaa gggtgggttg
    16441 cctgcggctc gcttgtcttt ccagaagagg aaacacaggg gaaagaagaa agcccggccg
    16501 gttgtcgcgt accgcgcgac gatgtggtct ccctcgaaaa ccagaccagg ttgtggcgtc
    16561 aagcttttcc attccggaac ggcgagcggc agaaccgccc cggcgtaatc ccccacggca
    16621 agctcaagct cttgggtttc gggtaacggg tggacggccg tgttgcgcga aagcccaatt
    16681 tccaactgat actcccaggt tgtgcctctc gaagcgagaa acgtctccgc aatgaggaag
    16741 aaatcgtctc tggggcaaag cgtgacctga cgttggtggc gaattcctcc ggacagtcgc
    16801 cgttcgtatt cgcggtggga aacctgtcgg tcgtcaaaac ggcagacctc tcgccagggt
    16861 gaagtcactc ttaactgccg atcgccgcac ttcagcgtgg ttgtacaaag ggtgacaatc
    16921 gtatttttgg ccagtgtgat gaccgattgc caatcctggc catcgacaga gatttccgca
    16981 tggagcgagc gccccggttt tttcggacct aggatatctt tcatgatgcg tgcccgaaga
    17041 agatgatcaa tccaacgtat agggtagaac aaggcgaatc cgccatggat gtgggttgcc
    17101 gcagtaatga tttcccattc tacccagatt ggtaccaagc gcggacccgg ccagggaaag
    17161 gcgtcttttt ctcgcggggc ccgcgccact gcgtccgtcg tgctacgagt tgagaatcta
    17221 ttgtcgcacc cggccaccag ggcgcgtgga tcttcattat gggtgggcca aaaaactgcc
    17281 tagattgcgc aaccggaata gattgtccga atttcctcga caatggtggt ctgccgttct
    17341 ggattgaatg accgttcgga atattccttg aatactgacc gcgccgatgg ccgatttgct
    17401 gtaataggtg acatcacgcg agaccatttg gccgggctat caatccggcg aaagccgtat
    17461 caaggcacca ctgaaccggc ggttgcacga tcactggacg aacaggggga aatcatgttg
    17521 cgacggatcg ttttggcgac tttattagct gtccttattg gtaccttctt ccaacaggtg
    17581 gcctccgccc agcaggctta tgggcgacaa tggggacgca cctacagcac ccaagactgg
    17641 cagcggtttt atcattaccc gtatgtctac tatccccaaa actattgggg cccggattac
    17701 taccgcagct cggaagatct ctattatcgt tatccacctg aaatgcgaat cccggtttac
    17761 aatcgcaact ggcagaattt ctacccagaa ccgcgacgct actaccaggg acaccacttc
    17821 atcatggaca tcttctaacg acacgaggca ggccaggcga gtactcgcca gtaaacaatt
    17881 cgcttgccaa ccggcgaaaa caaaaaacct gcctaagtac gatactcggg caggtttttt
    17941 cattggttaa aagcctttgg gccgacgcgc cattcggacc gggatattac tccggtatga
    18001 tggccttaag gatgtgctca acccactggt ttttggggat gtcatacagg ataaaatctc
    18061 catcaaattg ccaataggcc cagctccacc ctcggcgttc cgcttctcgc gtgacaaatt
    18121 gcagccaagt aacgcggtct tcctgcgggg ccttatcgta aacaccgaat tcacccaaat
    18181 agaggggccg cttgtgacgt tccgcccatg cttgggcccg atcgaagtcc gcccggaccg
    18241 ccgctttttc ttcctcgctg ccccaatgga tgccgacctt atcggccttt cccgcccagg
    18301 atgccccctg atgagtgaag tcgaagggac tgtaatagtg aacggtgacg atgatattcg
    18361 gatcgtccgc cgggagttcc aatttgtcga gggctgcgat gttattccac tggccggggc
    18421 cgatgatgac cgtccgtttg gggtttctgc ggcggaccag agccagggcc tctcgcgacc
    18481 attgattcca cagttcggga gtcatagcgg agttgggttc attgagcagc tcgaaaagga
    18541 cctcaggcgg gcggttcttc tgccgttcgg cgatcacctg ccagcaggcc atgtaacggt
    18601 ccttaagctt gtcgggattt cgaccgatgg ccgtgaattc gtgaaagtcc aataccacga
    18661 ggaggccggc ctttaaggcg tgatccacgg cccaatccat ggtctccagg tactccggtc
    18721 gcaatcccgc cccttcacca ccctggggat tgtcgcgaaa cggatgtaag ttgatccgca
    18781 catgctggaa gcccgcttgt cgaatgagcg ggaaaaactc ttcgcggaat cttgcccgcg
    18841 aacgatcccg ccacagcgga tcatagccga taatattcac gcctcggccc aggcgtttat
    18901 tttgagtaaa ggcatcgggc cactcagtct tcggagatgt ctcttccgcg aaactcaccg
    18961 agaacccaaa aggaatcgag cagatcaacg ccgaagccgt ttcgaggaag tgtcgtcgct
    19021 gcatagttca cctcaaggaa ggaaaaaacc atcgcctggg aggtttacga acgatggcaa
    19081 taccgggggt tgccaccaat tgaatcaaga taagcattac agggactgta ggtacttgtc
    19141 aacagcgcgg gcggcttccc gaccatgatt gatcgcgtgc accacgagcg aagccccctt
    19201 gacggtatca cctgcggcaa agaccccagg gacggaggtc atccagcgtg tcacgcggac
    19261 gttaccccga ttgtctagct caaggccgaa ttgctcgatc agtccatcgt gctccacgtg
    19321 caaaaacccc atggccagga ggaccaaatc ggcaggcacg gtgaagtcgg tatttggttt
    19381 ttcccgcatc tcccagccgc gaggaccgcg gacccactcc acctcgcacc catgcagatg
    19441 ggtgacctgc ccattgtgga cagtaagtgc cttggtcaag acactccatc ggcgggtaca
    19501 tccttcttcg tgggacgttg acgttcgcat aatccgcggc cagagtggcc agggcgtttc
    19561 ggggttcttg ccctcgggag gcttggggag tatctcgatc tgaagcacag acttggcgcc
    19621 ttgacgaatg gctgtgccca cacagtcact tcccgtatca cctccaccaa tgacgacgac
    19681 gtgcttatcc cgggcataaa tgctgtttgg gccgctggta gggatcggat cacccgccac
    19741 ccgccggttc tgttgggtga gataatccat ggcaaagtga acacccttgg catcagcgcc
    19801 agggacggtg agtggccgtg gcttcccagc gcccatggcc aggagaacag catgaaagtc
    19861 tcgtcgaagt tggtcagccg tgatatcgcg tcccacccaa acgttcggct caaaatggac
    19921 gccctccgcc accatttgct ctaggcgacg atcgatgacg tgcttttcta gcttgaaatc
    19981 gggaatgccg tagcgaagca gtccgccaat gcggtccgag cgttcgaaca cgacgacctc
    20041 gtgtcctagg cgacacaatt gttgggcagc agccaaaccg gcaggaccgg agcccacaat
    20101 ggctacccgc ttgcctgttc ggtgcgtcgg tttgagggga cggacccatt tctcggcaaa
    20161 cgccttttcc gcaatctggt attcgatatg tttgatcgtc accggctggg aattgatgga
    20221 cagcgtgcaa gcaggctcac agggggcagg gcatacccgg cctgtaatct ccggaaagtt
    20281 gttcgtggaa tgcaggttct gagctgcttc ccgccatcga ccccgataga ccaagtcatt
    20341 gaattccgga atacgatttt tcaccgggca ccccgccatg tggcagaacg ggatgccaca
    20401 gtccatacag cgagccgcct gctcgttcag gacccgctca gccagcggac gatcaatctc
    20461 atgccaatcg cggatgcgtt cggccacagg ccgatggtcc acttctactc gcggatattg
    20521 tagaaaaccg gttggattac ccactgaaaa cctcctctgt tgccgggagt tgttcggcgc
    20581 ggacctgttc tcgttcgcgg atacgttcca atgccttgcg gtactcgatg gggatgacct
    20641 tgacgaactt tcccacggat tccggccact tatcgagcaa tcgcttagcc tgctcactcc
    20701 cggtccaggc gtaatgccgt tcgatcaact gacgaagttg ctgctggtct tggggttgcc
    20761 atacggactc tagttccacg agatcgagat tacacaatgt gtcgaaaagt tgatactcat
    20821 cgagcacgta ggcaatcccg ccactcatgc ccgcggcaaa gtttcgccct gtcttcccaa
    20881 ggaccaccac cacgccgcct gtcatgtatt cgcaaccgtg gtcgccgaca ccctccacca
    20941 cggccgttgc cccactgttg cggacagcga accgctcgcc ggctaggccg ttgatgaaga
    21001 cttcgcccga agtggcccca taaagcaccg tgttgcccac gatgacgttt tctgcgggat
    21061 caaacggact gcctggtggc gttcgaacga caatccgccc accggaaagc cctttcccta
    21121 aataatcatt ggcatcacca atcagctcca gtgtgatccc aggtgcaaga aaggccccga
    21181 aactctgtcc ggcagaaccg tgaaatacca agtgaattgt cccatccggg agtccgcgac
    21241 tgccgtgttt cttcacgatg tgataactca gcgttgtccc cacagtgcga tggatattgc
    21301 ggataggcat ttccagccgc ataggctgca tgttgtcaat ggccgcttgg acctgcggca
    21361 aaagcacacg atctaaatga tcttgatgct tgtcggtctg cggacgaacg cggcgaaggg
    21421 gcgtgccttc cggcactggg ggtggggcca gcaccgcaga aaagtccaaa cctttcgcct
    21481 tccagtgatc aatagcgcga cgcacgcgga ggcgatcgac ccgaccaacc atgtcgtcaa
    21541 aacgtcgaaa gcccagttgg gccatccagc ggcggacttc ttccgccaca aacaagagat
    21601 agcgaataag atattccgcc tttcccgcga accgtgctcg caactctggc atttgggtgg
    21661 caatccccac gggacaagtc cccaagtggc atttccgcat tagaacgcaa cccaatgaga
    21721 ccaaggccgc cgtcccaaaa ccaaatcgct cggcgccgag caaggccccg atgacgacgt
    21781 cgcggccggt ccgcatttga ccatccactt ggaccagtac ccgatcgcgg agaccgttga
    21841 gcactagggt ctgttgcgtc tccgccagcc cgatttccca aggaataccg gcgtgcttga
    21901 tcgaagacca cggactggcc ccggtgcccc cgtcgtggcc actgatgagg atctcatcgg
    21961 catttccctt ggccacacct gctgcgaccg ttcccacgcc tacctcagcc acgagcttga
    22021 cggagaccat ggcctcgggg ttggcacact ttaggtcgta aatcaactgg gccaggtctt
    22081 cgatggaata aatgtcgtgg tggggcggag gcgagatcag ggacacgccc ggcatcgcgt
    22141 gccgcaatct cgcaatttct tccgtcacct tatgaccggg caattgtccc ccctcacccg
    22201 gctttgcccc ctgggccatc ttaatttgca gaatcttggc attggcaagg tactcgatcg
    22261 ttacaccaaa tcgggcactg gctacctgtt taatcccgca attgagggag tctccgtttg
    22321 gcagcggacg ataacgggcc gggtcctccc ctccctcgcc cgtgttcgac agggcgccca
    22381 agcggttcat ggcaattgcc ataatctcgt gggcttcccg gctaatagaa ccatgggaca
    22441 tggccccagt gtagaagcgt cggacgatcg agctggccgg ttcgacctct tccagcggaa
    22501 cgggctcgcc aggctcaaac tcgaacagtc cccgcagcgt gcaaagctct tcgttttggc
    22561 aattaactcg ctcggcaaac tgaagatagg tctcccaatc gttgttgatg acggcatgct
    22621 gaagctttga gacggtctcc gcattccaca aatggcgttc cccgtcaacc cgctcgtggt
    22681 attccccgcc aaaatcgagc ggcagagcgg ccggttctcg cggaccgaag gcgtcgcggt
    22741 gtcgggccaa ggcctcctcg gcaataacct gaagatcgat cccgccgatc cgcgaagcag
    22801 tgccgcaaaa gtatgcatcg accacagaac gatccaggcc aacggcttca aactgctgtg
    22861 ccccgtggta actccgcaag gtggaaatcc ccattttcga catcgtcttc aggattccct
    22921 ttttcaccgc cgtgatgtac tgctcaatgg cctcgtcaag gctgaaattc ggcggcaggt
    22981 ccttgttgcg gtgaagcgct gcgatggcct caaatgccat gtacggatta atagcgttgg
    23041 ccccatagcc acacagcagg caaaagtgca tcacctcacg gggttcgccg ctttccacga
    23101 taattccggc ttcgcctcga agtccgcgac ggagtaatcc gtgatgcagg gccgaagtcg
    23161 ccagaaggct cgggatcggc acccattccg cattcacgcc gcgatcgcta agaataatga
    23221 gcgaggcccc atcacgaatg acacgctcgg ctgagtccac aatcttctcc agcgtttcgc
    23281 ggagggttgc cccagggttt tcgcacttgg cgggcatgag gatcgaaacg gtggcgacct
    23341 tgagatgcgg atggttggcc tcgcgcaatc gccgaatgtc atcgttcgtc aaaatgggat
    23401 gcggcagctt cagttgtcgg cagtgctccg gtgtctcgtc caaaagattg cgatgctttc
    23461 cggtgaacga catcaaagac atcaccaggc cttcgcggaa tggatcgatg ggcggattgg
    23521 tcacctgggc gaaaagctgc ttgaaatagc taaacaacaa cttgggctta tcggaaagca
    23581 caggcaaagc tgcgtcgtta cccattgacc ccacgggttc ctgcccgtcc cgtgccatag
    23641 gcgcgagaat catgttcagg tcttctaccg tgtacccaaa tgcccgcaaa cgttgctcca
    23701 acggtccctt ctcggattca acatcggtgg ggggaaggaa gagcccgcgc agttcaatcc
    23761 ggttttgttc cagccagcgc cgatagggcc gttgtcgaat gatcttcccc ttgacctcgt
    23821 tgtccgaaat aatccgctgt tccttcgtat ccaccaggaa catcttgccg ggttgaaggc
    23881 ggcccttctg acgaatcttc tccgggggaa actcgatgac accggtctcg ctggcgagga
    23941 tgaccagtcc atcatccgtg acaacataac ggcaaggccg caagccgttg cggtccaacg
    24001 taccccctac cagtcggcca tcggtaaaga cgatcgcggc gggcccatcc caaggctcca
    24061 tgatggcggc atgatactcg taaaatgccc gcttgtcggt gctgatgtga taccgcgggc
    24121 cgaaggcctc ggggatcatc atcatgaggg catgtggcgc cgatcgccct gcccgaacca
    24181 atagctccat gcaattatca aacgccccag agtcacttgt gcccggctcg atgatgggac
    24241 aaagcctgct caaatcctgg cccaatagcg gcgatgtcat cttcttttca taagccgcca
    24301 gatggttcac gttgccacgc agtgtgttga tctcgccatt gtgtgcgatc attcgaaaag
    24361 gctgcgccaa tttccaactg gggaaggtgt tggtgctata tcgctgatga acgatggcca
    24421 aggccgattt cagacgagaa tccgagagat ccggataata tgcggccaat tgcggggcga
    24481 taaacatccc cttatagacg atcgttaggg aggacatgga agggatataa aagtcggccg
    24541 cagcttcccc aagttcgccc cggactcgat gttccaagcg ttttctcgtg atgaacagcc
    24601 gccgctccaa ttcctctccc cgaagtccgt ttccgtggat gaagaattga aagattcgtg
    24661 gctcacaggc ccgggcgatc tcgcccaaac aggaattgtc ggtagggact tcccgccgcc
    24721 cgataagcgt tagtccagag gtgacgacct cgctttcaag taatgtttcg cagcggagcc
    24781 ggacctcgtc ttcccggggg aaaaagacca tagcgacgcc gtactcacca ggagccggca
    24841 ggtcaatttt gtcggaagcc atttcctcca ggaagaagtc atgcggaatc tgaaagagga
    24901 tgcccgcccc gtcgcccgtg gtttcgtctg ccaccgaggc cccgcgatgc atcaggttaa
    24961 gcaaaatctg tttgccgagg tcgatgatac gatgatccgc cttaccggat atgttgacca
    25021 ccgcaccaat cccacacgca tcgtgctcgt attgtgggtc ataaagtccg taagcctgtg
    25081 gcggtcgtct tcggttctgt tgattcattg ccattcaacc ttgattgcgt agtatcgtcg
    25141 tttcgtgaat tgtattgcga agcaggacaa agttaacctg actttggcgc tgttttgatt
    25201 gtgagaggct tttcccttgt gcgggcaacg gcgtcctggc agcgtcggcc ctaataggac
    25261 ccagcagtcg cacacgtgat gaggctgatc acgtgccgat cggacgcctc agcagacgcc
    25321 ccattcctca tcattgtcaa ggtgccatga gaacttgcca aggctgctct tgttatgtga
    25381 cggggcaatt tcgctcaagg ttcgcggcaa agagttgcca caagatcggc taaaaagcct
    25441 ccggggagat gaaggagtta acgagtgaaa ggtcggccaa tgcaatacac gaactgccga
    25501 ccgcgaatga agatgtgccc atctgacacg gcgggcgatg cgatgctccg atccttgacc
    25561 tcgttcacgg acaagacctc gaaggttggc gccgcccgga cgaccgtgca tcgcccagag
    25621 gtatccaaga agtagatgcg atcctcggcc gccagcggtg atgccttaaa ctcacgtccc
    25681 agtcgctcac gccacttcag ttcgcctgtc ttggcatcga ggcattgaac gatgccgttg
    25741 tccgcggcga ggaacaagag cccgtggctg taaacaggtg aaggactgtc gggattattc
    25801 ccttcgtagc gccaaaggat tctctcaggg ggcaaacgcc cctcgccgcc gatctttgcc
    25861 gcgaaggtgg cgccccgcat gccgatggtc gcgaaaacga tatcgttctc gatggttgga
    25921 cccgtgatcg tccgattacc tgtcaggccg gggagatacc aaatttgctt gcctgtccgt
    25981 gcgtcgtagg catccaattg ctcggccccc atcacgacga tctcggtgcg atcgggcaag
    26041 cgacgcacca gcggcgtggt gtaggcgtca caactttctc gcatggcccc cgtcatcctt
    26101 ggtgtgtacc agacttcttt gccggtcttc agatcatgag caacgacata actggggtgg
    26161 ggcttatctt ggaggtcggc cagggaatcc tgcatacata ccgagacaac aagcccatca
    26221 acgatcaccg ggctattggc gtggccccac caaatcgtgt atttgccgta atcggcctgg
    26281 agattgcgct tccaaagaag ctcgccgtca aatgtaaaaa cggccagatc gccattgcca
    26341 aaatgggcaa cgacgtgctc accatcggtc acaaccgagg ggctggcatt attgttcaga
    26401 cggtgaaatt tctgttcgcc tcgtggcttg ttctcgggag tctccgccgt tccaacctgg
    26461 cgggtccaaa cgatcttgcc agtggtcttg tcgagtcgca ggaccaaaag tcgatcctcg
    26521 gcctgcgtgg tgatgaacag cgcgttttcc caaatgacag gcgtactatt tccccattcg
    26581 ggaatggcgg tcttccacac aatgccctgg ttggcttcct cggaccatga tagcggaagg
    26641 ttcgtctcgc gggagacacc cgtgccctcg ggtccgcgcc aggcaggcca attgtctgcc
    26701 cggctgatca tctccggccc aaaaagtacc aatatcacca aaaaccgggc atttatccgg
    26761 cacaaacttg ccgaaaatcc gtcgaaaaaa tttttttgct ttatcattga cccaccagtt
    26821 tttggaccgc taaggtgttt tgggctcgtt tagtcggggt ccatgcccca tttggggatt
    26881 actcgtcttt tgcacggagc caagactgaa tggtaggggt gtaggggcac agctcttcgt
    26941 tattgaagaa aatcgccaat tcccgggcgg cggactccgg gccgtccgag gcgtgtacga
    27001 gattcatctg gcgcgagcag ccaaaatcgc ctcgaatagt ccccggtgcc gcggccaggg
    27061 cattcgtcgc ccccaccatc tcgcggacaa cacggatagc ttcgggcccc tcaactgcca
    27121 tcgccacgat tggccccgaa gtgatgaact cctccaacga gggataccat gctttgtgaa
    27181 cgtgctccgc gtagtgcttt cgtgcgagtt ccggcgttac gcggatcatc ttcatggcaa
    27241 cgatgttgag gcccttgtct tcgaaccggc tgatgatccg acccatcaaa cgacgttgca
    27301 cacaatcagg cttcaacaaa acgagtgtac gttccatgtt tctct
//
LOCUS       DAOSUT010000042        26816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1187_length_26816_cov_9.471283, whole genome shotgun sequence.
ACCESSION   DAOSUT010000042 DAOSUT010000000
VERSION     DAOSUT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 26816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26816
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1187_length_26816_cov_9.471283"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLD05_05550"
     CDS             <1..433
                     /locus_tag="PLD05_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76934.1"
                     /translation="IPLILVTTVWYALTAVMGTSLHHHLGWHPVVGTPVSSSGCYGSC
                     GFKDSEGSYLLSSTAFAKDCNTANIGRHLRMVATFRTTVGAHQWSQCPICQFQVQPRT
                     IQNIFQYVAGAYSCWVSQPRGVAVVVFTPDFSWHSRAPPTV"
     gene            660..1634
                     /locus_tag="PLD05_05555"
     CDS             660..1634
                     /locus_tag="PLD05_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP76935.1"
                     /translation="MLRPIDGSNSDFKSRSDQTHIGHLGFTLVELLVVIAIIGLLIGL
                     LLPAVQAAREAARRAECSNNLKQIGLAIQMYYDTNRRLPAGWTAYTADGRQPQVNGQP
                     GWAWGAVILPYLEQMNLYQGGIVLKAPIYDPVNEQAIQTFLTIYRCPSDLSQDRVFEA
                     HKLHEGHDHDQGHAGDEYDELLLARSNYVGSFGTTDIHVCEDTPPGGSCKGDGVLFHN
                     SYLDYSAISDGLSQTFLVGERTVALGYATWTGVLPGDACAPAAVLASAESPPNLRKAH
                     AHNFSSQHPQGANFLLCDGSVHMISQTIDLNVFRALATRSGNETPGPF"
     gene            complement(1655..2848)
                     /locus_tag="PLD05_05560"
     CDS             complement(1655..2848)
                     /locus_tag="PLD05_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP76936.1"
                     /translation="MSHVLRVAIVDPHDAGREELKRLLLGLDSIWLEAECSRYEFFAD
                     VVAQTNPEIGIVALDFDQEKALDLVSKVTQQSPDCAIFVVSSSHDGTVILKAMRAGAK
                     EFLPQPVRIDDLLAAIERLTERRFGRGDHKARGSTVIAMTGVTGGVGVSTIAVNLGCA
                     LARNDKNSVVLVDLDLCLGDADVLLDAIPDYTIVDVAQNVTRLDFSLLKRSLTKHSSG
                     LFLLPRPIQLEDVALVSAEDIQRVVGLLKASFTHLVMDLSKAFTPLDMMAMEMAQHIV
                     LVTQLDLPCLRNVVRLLMSFNNIQGMAEKVKIVVNRTGLESGQINIKKAEEIIGREIF
                     WQIPNDYRACSESRNNGVPLLESAPRSPVTHAIVGLAEALCGESAKTAEKKPAKSGLF
                     KLFAK"
     gene            complement(3124..4842)
                     /locus_tag="PLD05_05565"
     CDS             complement(3124..4842)
                     /locus_tag="PLD05_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOP76937.1"
                     /translation="MIRLKGRKRLLLGLAITLALVAALPATGEVAPGPPEPSSPNQVV
                     RQITGPSQREEMTVNTSRILTLDKRIPQAQVNNPDILDITALSPTQIQIFAKKAGVTQ
                     VNLWDEKGNIYTIDVIVFGDARELDLLLKSQFPNCSIKVIPIASGVLLSGYVDQQEHV
                     SRIIQIAEEYYPRVINNLTLGGVHEVLLHVKVMEVSRTKLRALGFDWTQISGQNVIRS
                     SVSGLISAANAANPAAGTAATAVTSGGETFFFSVFDGNDAFFGVLEALRQDNLMKVLA
                     EPTLVTVSGRPAFFQVGGEFPIIVPQSLGTVSIEYKRYGTQVDFVPIVLGNGRIRLEV
                     RPRVSEIDSSRSVVINSTTVPGLRVREVDTGVEMQAGQTLAIAGLVETRVEAQRRGVP
                     VISDLPYIGSAFRRVAERSNDVELLILVTPELVDAMNAEEVPPCGPGTETASPTDWEL
                     FMKGYIEVPRCCDPSATNGAVNPAAGQPTPAPGPSAQSAQTGNAASVRVARSNDGSGV
                     PGLQAASPYAVSEGSSTTTFGKPGVVPQTRSARFVNDSSSPKPQPRPSEPSPLPGFKG
                     EIGYDN"
     assembly_gap    5120..5219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5220..5812)
                     /gene="cpaB"
                     /locus_tag="PLD05_05570"
     CDS             complement(<5220..5812)
                     /gene="cpaB"
                     /locus_tag="PLD05_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOP76938.1"
                     /translation="MRTKSVLLLLLALGCGLVAAIGVKQVLATKDQATASPTVEMQAI
                     VVAAEDIAMGDPVKIEQIKLEDWPKSKIPEGALFKAEEAEGRRTKTKIYKGSPILDDQ
                     LLAKGAVDAGAAPWIPKGYRVVSVKVDAVSGSASMLRPSDRVDVVVHFPANPNKGIPK
                     DYTRTILQNVRVFAVDDKYILTEDEKTKKSLPAKTVSL"
     gene            complement(5857..6366)
                     /locus_tag="PLD05_05575"
     CDS             complement(5857..6366)
                     /locus_tag="PLD05_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOP76939.1"
                     /translation="MRFASLSKSPALTNCQDRGMGETSQARARSRRWLRCSPRTPRAG
                     VAVVEFALVAPLFFLLVLGMIEFGRMVMVQQVLVNASREGARAGIVQGATNTDVLAVV
                     QSYLEGGGIHGATVNVSPDPIDLAGYGDPVTVAVSVPFRQVSWLPTPIFLGDTELRAT
                     TTMRREAIQ"
     gene            complement(6402..6980)
                     /locus_tag="PLD05_05580"
     CDS             complement(6402..6980)
                     /locus_tag="PLD05_05580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOP76940.1"
                     /translation="MLERLTETADQPWMIWAVCWFVSVILVVAAWIDGRKLKVPNWLT
                     FPLILSGWAASGYFFGWSGLYSSWLGTAVGLGLLLPAYAIGGMGAGDVKLLAGVGAWI
                     GMAHTFWAFVLTVVIGAILAVAMVAARGAWGKHIRQFFAILFEILIVRDPEKLSALAA
                     ERKPKMLLLPYGIPIAIGTIVYFLVNGMLFPV"
     gene            complement(7333..7524)
                     /locus_tag="PLD05_05585"
     CDS             complement(7333..7524)
                     /locus_tag="PLD05_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOP76941.1"
                     /translation="MRSLALKLQRFLVSEDGPTAVEYAVMLALIVVVCLTAISSLGTS
                     ANSTFQSVSDSIQSTAGGG"
     regulatory      complement(7792..7875)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8479..9711
                     /locus_tag="PLD05_05590"
     CDS             8479..9711
                     /locus_tag="PLD05_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP76942.1"
                     /translation="MNVIENQSVDQIARESPTSLEVANEAQPPVGVIPSESPTALPTT
                     LLVRAVLWLIAFVSIILAVPYLAEEISYAIVRGRLRAEADSAKAWLQQAPELENRYRH
                     VIKTVFPGVVGIETVRETTVARLPRRPFPLPDFREESMGSGVIVDTQGYIVTSLHVVE
                     QAKEITVRLADGRLIKDPTIVGTDPLNDLAVLKIPSKGLMAVPWGDSDELEVGDTVLA
                     IGNPYGLTRTVTAGIVSAKERRAQSQAGGFQEFLQTDAAMNPGNSGGPLVNLRGEIVG
                     INSAIYGEAYRGISFAIPSRVARHVYEQIRRDGQTRHGWLGVQMGDVDQSTAQAHRLD
                     RPQGALVLSVLPGSPAEAAGIQPGDIIIAWDGKPLEDAADLGVAVARTPVGSEVTVEL
                     HRGQELKKLTVRVGQRPM"
     gene            complement(9931..13026)
                     /locus_tag="PLD05_05595"
     CDS             complement(9931..13026)
                     /locus_tag="PLD05_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOP76943.1"
                     /translation="MKIADFAVRRPVATSCLIIGLTLLGLNAFRKMGLELMPKIDIPY
                     ITVVTIYPGASPEELEIDVAKRIEDAVSSIEGLKHVSSICMENVCQTELEFEMEVDVD
                     IAATDVREKIDLIRSELPEDVEDPIIQKFDINAKPVATMALVGDVPLDELYDYADNEL
                     RDRLTVIQGVADVQLIGGAKREVQLRLDREKLAARGLTTMDVVKAVQASVKTIPAGRI
                     REAGTEYSVKFDADYETVAGLNDLEIANHEGKRCYLRDVGEAVLATEEVRQLATLDDK
                     QCIAIKVIKKADANPVAVANAVKQALERINRELPSGMQLVWVTDDATFIEAMNLDAWK
                     NVAIGIGLTAAILFFFLYELRSLIIVSITMPLTIIIGLFFMQAVGYTLNTSTLIAIGL
                     SVGILVTNSIVVLESIIKRLEELGDPKEAARIGANEVFVAVLASAITNVVVLFPLAVM
                     KTRVGLFIAPLAMTMFILTLVSLFISFTLTPMLCSLILKPQKRSRSLLRRIGEGFNRF
                     LEANVSAYRWLLGFTEHYRVVGALVLLAVVGMFVHSLWVAGRLGTGFFTETDRGEVYV
                     RLEFPTYYDLAATTAKINEAKRRLEGLPELKHMLTTIGKAEAVLGQSSEGVYLGQMLL
                     VFSQRTERELTIYQLMDIVEQRLQDFPDARIVVSQASLMGGQSNPVEMEIAGPDLQKL
                     DQLALASLRLAREIPGVLSPDTSVREPKPEIRVRPRRPILADINSPAVGMGLTLRGNL
                     EGIKAGTFKSRETARNYDIVVKFMEQEGKQQVAGFMFPGVEGHALILATLGTIEERKI
                     PIQIVRKDKQRISKVTAQLAPDLPLGKAVTLISERVNREVQLPPGYRYSFGGIYEVMQ
                     EGLGGLFEAFIISVLLVFLSLAAIIESFKRTFLVVVTVPMALIGTFWALAIAGQSLDI
                     FAVMGIVMMTGIVVNNAILIIDQFNVLIAQGVPRHQAMIQAACDRFRPIVMITLAAVL
                     GMLPLALGRGIGAEPRVGVGVATVGGILVSGILTQIVIPILYDVATRGGNNRKMAAHT
                     A"
     gene            complement(13023..14267)
                     /locus_tag="PLD05_05600"
     CDS             complement(13023..14267)
                     /locus_tag="PLD05_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOP76944.1"
                     /translation="MNRKKLLQGIISLGFAALVVGVGIALAMLHSWMFGDEKTPSATQ
                     TSAVSSGAKVGDRDAVPVVVQAARTMVFEHRLDVSGNVLAKRFALVSARIPGTLDAIF
                     VDEGDFVEAGKTKLFQTDSVKLTQAVAIAQEQVTVAECAVREKEALLEKTLVAQQVAE
                     ADLARYKDLAQRNAIARQVVEHQEAQVRQLQADVRHVQTLIELSKAQLEQARLNLKIA
                     EKDLGDSLVVAPISGRVSLRMKEPGEMAGAGTPVLRIDDLSLVEVSIFVPSEYYDQVV
                     VGETKLRARVAGRDLGELPVTFKSPVIESRLRTFQVKALVQAPPPEVVPGALAQVSLI
                     LGRRQGLGVPKQAVQQRSGKNIVFIVEDNKAKAVAVELGWETEGWQEIRAGMSAVAPV
                     ITSGQSLVDDGTPVRVLEEAGR"
     gene            complement(14433..15146)
                     /locus_tag="PLD05_05605"
     CDS             complement(14433..15146)
                     /locus_tag="PLD05_05605"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOP76945.1"
                     /translation="MAPSLTEKCRELAVSAMKDGISQAALEVLKQYGYEGLTMDRVAE
                     VAGIAKGSLYHYFRNKQELVTYLFEQTIEPAIQAGEEIFQRVVPAVEKLEAMVRMWFD
                     YFSQHRALFDFLFRDPTVRELCLTSRRSKNGLAIDRFSAILRQGINEGVFRPLDVTYA
                     AEMIIGSLQFVIERQLETGEAHPIEVSVRRLMDIVLHGIGASSASAESPSAIGQRCEA
                     PQVEGKTGESSEPSQVAAE"
     gene            15734..17626
                     /locus_tag="PLD05_05610"
     CDS             15734..17626
                     /locus_tag="PLD05_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOP76946.1"
                     /translation="MNERTRPGRLEATDCRTAIARRNLPVLRVDKLPITSLAFRFSSF
                     IFLILLTAPGCSTSLREWWANGFKVGPNYCRPSAEVGEAWIDSDQPGVKTSGDVTYAH
                     WWTVFNDPTLNELVYTAYLENLPLQIAAWRIMEARYQLGVARGYLWPQEQQAMGAFSR
                     NQISKEGFPMSKFSEIPGFKMSYDDWVAGFSLAWELDFWGRFRRTIESAEAHVQAQEE
                     DYNAALVLLQAEVASTYIQLREIDERLELARKNVALQKDTLAIAKARFEQGVVSQLDV
                     HQAQAILSNTESMIPMLEAMRRVSENRLCTLLGQPPQDLDPILGPSGKVPDVPPEVVV
                     GIPADLLRRRPDVRRAERLAAAQCARIGIAEAEFYPHIALTGQLAYQAENFSDLFSGR
                     AFTGMVGPGFRWNILNYGRIRNSVLMEDARFRQAILGYQETVLRAAEEAEDMITAFLM
                     EQRRLRALQQATEATAQASQLAVAQYQQGLIDFQRVIDSQRALVQQQDALAESRGKVA
                     LNLIGVYKALGGGWEMRRNPSAGIVLTRLQTKTGGSTQPAPAPQPTAPSSSGGTLPMA
                     PTDGQNVSPPAAPAPPPEKAPAELPTSPAPPQLPPAPTTRWGDQDVPPMVVPTVGELP
                     EINISG"
     gene            complement(17859..19931)
                     /locus_tag="PLD05_05615"
     CDS             complement(17859..19931)
                     /locus_tag="PLD05_05615"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOP76947.1"
                     /translation="MFVRWYGPALVVLGFVWWASSLASPASEGGTPPGPATHIVEDGG
                     QWVLENAILKVTLNPQTGAWEVLDKRVGHTWASFVEDPKRQNVLRNIKTLDDETLSCE
                     TTIFWRDNKPIAGTVTLRLPRDSADLFMTVDMPDRDVAFSNQRFWTPLFYHSPDGAIA
                     IADYCNGHLYPVAEEQLPRRWFNTSRLDMPWVGVCDVKTGIGYMIIAETSDDGYLELR
                     KVEKDGQSAWVPWIGWSPSMGKFSYPRKLIWHFSDQGSYVALAKRYRTYAQQLGLIVP
                     FAEKLKANPELRRLFGAPDVWGNANLSFAQEAKNAGIRRLLVQGRMPPDQMKQVNELG
                     YLTSEYDNYTDVLPVENEDQIDRSHDLVPQNVVLKSDGERMKAWLTFDKKTQYMKRCP
                     ALWLRTAQRDIPKILSTYPFLGRFIDVTTAEDLYECYDPAHMLTKAQKRQCGVDLLSY
                     VRSLKLVVGGEHGIWWAVPYVDYFEGLMSCNAYFSWPAGHLIPPKRKDEEFTSPWGNK
                     LPPWSQYERWGIGHRERAPLWELVFHDCVVTTWYWGDSSDFLLQAAPEITPKKDAFNV
                     LYGTIPLLWADRRKGSWLNNREVFLRTYRNTCILHEVIAGTEMLNHEFLTDSRDVQRT
                     TFSDGTVAIVNFGSQPYELHVGDKSYLLPQNGFYVKGLRIEQIRELVNGRVVTTINSQ
                     DYQYREEE"
     assembly_gap    20138..20237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20238..20852)
                     /locus_tag="PLD05_05620"
     CDS             complement(<20238..20852)
                     /locus_tag="PLD05_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOP76948.1"
                     /translation="MPEVRLERLRPQEIQDRMNACGTLFLPIGTIEWHGLHNVVGLDA
                     IKAHMLCIRAAQRGGGLVAPPLFGGVGGLNQPHTFVMEPEDQIYSVLLRPWLEKLTME
                     AARQGFKAVIILTGHYGAAQQIVVREVGARMTRCLGIPVLATPEYILALDEGYLGDHA
                     AWGETSLMMYLDPPTVDLSRLGEPPHQGVGGRDPKQFANAEDGRR"
     gene            complement(21150..22070)
                     /locus_tag="PLD05_05625"
     CDS             complement(21150..22070)
                     /locus_tag="PLD05_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOP76949.1"
                     /translation="MESLEIDTVYCLDCIEGMRKIPDGTVDLVFADPPFNIGYQYDVY
                     RDELDDDEYLDWCLRWTGEIARILKPDGTFWLAIGDEYVAELKTMIQREHRLVLRNWV
                     IWYYTFGVHCKNKFTRSHAHLLYWVKDPKRFTFNADEIRVPSARQLVYADRRADPKGR
                     IPDDTWILRPQDLPDGFRPEEDTWYVPRVCGTFRERQGFHGCQMPEQILGRIIRACSR
                     KGELVVDPFAGSGTTLAVAKKLGRRFLGFELSPDYTAKIRERLASIREGDPLEGSAEP
                     LQSAPPTARGRRLAMKEPDGQGPSRFLFES"
     gene            complement(22263..23210)
                     /locus_tag="PLD05_05630"
     CDS             complement(22263..23210)
                     /locus_tag="PLD05_05630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP76950.1"
                     /translation="MTSYRPFRNYDPPKLLRIWSECAGKPGLIQPLTLPYLERSVLNK
                     PYFDPNGLFLAFEDDQPVGFAHAGFLPQATQGELAREQGITCMLMVSKACDQQVVANE
                     LLDRCEDYLARGGARVLYGGSPNPCHPFYLGLYGGFRLPGIMQNDTLLTTLFGARGYQ
                     VVGGYLRFQVSRDQYRHLLDSNIIGRFSGFNIQQTTDPVPVSWCEALCFCESEIIQYT
                     LINPANQQPIARVRFCRLDPNPHPRPAVGLAEVRTAAGWANTGADSFLMEESFSSLWA
                     EGIGVVEAQCCQSDVTMVQMLREFGFQQRTSGVVYRKSI"
     gene            23965..25764
                     /locus_tag="PLD05_05635"
     CDS             23965..25764
                     /locus_tag="PLD05_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOP76951.1"
                     /translation="MLKVNLVQLPIPPLGPEIVRGNVPLASAYLKRYAQRVAGVDVEI
                     EILTADLTDRAGDAALVRELSQRGPNLVGFSCYLWNVERSLWLAERLKALHPELRVVI
                     GGPEVTLDNTWVLEHPAIDWAVVGEGEATFADLLKALTAEPRWLQHPTELPPIPGLWN
                     RATHSFGGARSQLPTLNEVGRVYLDGLLDPRWHDTLFLETIRGCIFQCAFCYYPKSFE
                     GLRFMDEDLVLANLHLAHQAGISEVFLLDPTLNQRRDFLSFLKLLARGNPERRFSYSA
                     ELRAEGIRAEHAGIMKAANFAEVEIGLQSVSREAQGLMHRRVNYQAFEEGVRALLEAG
                     IRVRADLIIGLPGDTAETIRQGFRYLLSLGLSNEPQIFRLSVLPGTELRRRSKELGLD
                     YQPHPPYHVLGTSKLSIVEIAQLMREAEFSFGRRFDPWPVSDEEFSPTILKERGSFWE
                     INLDRPEPTELPDIQIAHTLWIKARHFAGRAKDVRYWADRVLNENPHVTLWIVLDISD
                     AQTLPEEDDIQSILRACYDREHSYWDWYFAPELPPGPSGAKRLVVYSGRSLKGRRRWL
                     RQRFSPSADLVEAPECQAKGRGRRRRTCDSGNE"
     gene            25778..26188
                     /locus_tag="PLD05_05640"
     CDS             25778..26188
                     /locus_tag="PLD05_05640"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOP76952.1"
                     /translation="MESEPKTQIVEQVAIVVVCRDDHVLVGIRPDGVPFAGLAEFPGG
                     RLQPGESWEEAARRETLEETGLSVRVEALLGTVSTRYEDRILELRFFAASPVSPAASP
                     REPFRWIRRDALRADCFPPANRTIIEQIQKGHGQ"
BASE COUNT         5912 a         7943 c         7152 g         5609 t
ORIGIN      
        1 cataccgctg atcctcgtta ccacggtgtg gtacgccctg acagccgtga tgggtacgtc
       61 gttacaccat cacctgggct ggcacccggt ggttggtacg cctgtcagtt cttcgggatg
      121 ctacggttct tgtggattta aggattccga gggcagttat ctcctctctt cgacggcctt
      181 tgccaaagat tgtaatacgg cgaatatcgg tcgtcatctg cggatggtgg ccaccttccg
      241 cacgactgtt ggcgcccatc aatggtcaca atgccccatc tgtcagtttc aggtccaacc
      301 gcggacgatt cagaatatct tccaatacgt cgcgggagcc tacagctgtt gggtgagtca
      361 gccccgcggc gtggcggtgg ttgtttttac accggatttt tcctggcaca gtcgtgcccc
      421 tcccaccgtg tgaatgctgc cttgtcaaag ttcgctgact ccagcgacga agccgtggcc
      481 cgaccggtca attggcgttg attacgggcc tggcaacgca tgccccagaa caacatgtgt
      541 gcgtggcagt gttgccaacc agcaatgacg cgcatgctgg atgatacgtg agcattgaga
      601 cgttttcttt tcggcgattt cttcgacgca gttttttcgc acggttgagg aggttttcca
      661 tgctcagacc gatcgacgga tcaaatagcg attttaaatc ccggagtgac caaacacaca
      721 ttggccacct tggtttcaca cttgtcgagt tgttggtggt gattgcgatc atcggcctgc
      781 tgatcggatt actgttgcca gccgtccaag ctgcccgaga agcggctagg cgggccgagt
      841 gctctaacaa cctcaagcaa atcggcttgg cgattcaaat gtattacgac accaaccgga
      901 gattgcccgc gggatggacc gcctacaccg ccgacgggcg gcagccccaa gtcaatggtc
      961 aaccgggttg ggcctggggg gctgttatct tgccctactt ggagcagatg aacctctatc
     1021 aagggggcat cgtgcttaaa gcgcccattt acgaccccgt caacgagcag gccatccaaa
     1081 cctttttgac catctaccgc tgcccatccg atttgagtca ggatcgagtc ttcgaggccc
     1141 ataagctgca cgagggccac gatcatgacc aagggcacgc gggcgatgag tatgacgaac
     1201 tccttttggc gcgttccaac tatgttgggt catttgggac aaccgatatc cacgtctgcg
     1261 aagatacgcc acctggcggg tcatgcaagg gagatggagt tcttttccac aacagttact
     1321 tggattattc ggccatctcg gacggtctta gtcaaacgtt tttggttggg gagcgcacgg
     1381 tggccttggg ctatgccaca tggacgggcg ttcttcctgg tgatgcttgt gcccccgctg
     1441 ccgtgctagc ctctgctgaa tctcccccga acctgcggaa ggcacatgcc cacaacttta
     1501 gcagccagca tccacagggc gctaatttcc tgctctgtga cggctcggtc cacatgatca
     1561 gtcagaccat cgacctcaac gtgtttcggg ccttagccac ccgatcgggc aacgagacac
     1621 caggaccgtt ttgagcccgc ctggggtgtt gccatcactt ggcaaagagt ttgaagaggc
     1681 cgctcttggc tggcttcttc tcggcggtct tggccgattc gccgcaaagg gcctccgcca
     1741 gtccaacgat ggcgtgggtc acaggagaac ggggtgccga ctctaagagc ggcactccgt
     1801 tattgcgtga ttcgctacat gcgcggtagt cgttcggaat ctgccagaag atctcccggc
     1861 caatgatctc ttcggccttc ttgatgttga tttgaccgct ctccaggccg gtgcggttaa
     1921 ccacgatctt gaccttttcc gccatcccct ggatgttgtt gaaagacatt agcaaccgga
     1981 ccacattacg caagcaggga agatccaact gtgtgacgag aacaatgtgc tgggccatct
     2041 ccatggccat catgtcgagg ggtgtgaagg ctttggaaag gtccatgaca agatgggtga
     2101 aactagcctt gagcaagcca accacccgct ggatatcctc tgcgctcacc aaagcgacat
     2161 cttccaactg aatcgggcga ggcaacagga ataggcccga actgtgtttg gtcaaggaac
     2221 gtttgagcaa ggagaaatct agtcgggtca cattttgggc aacatcgacg atggtgtagt
     2281 ctggaatggc atccagcaag acgtcggcat cgcccaggca caagtccaaa tccactagga
     2341 cgactgagtt cttgtcattc cgcgctagag cacaaccaag attgacggca atcgtcgaga
     2401 ctccaacacc gcccgtcacg ccggtcatcg cgatgacggt agatccccgg gccttatgat
     2461 cccctcgccc aaaccgccgc tcggtgaggc gctcgatagc tgccaggagg tcatcaatcc
     2521 gcaccggctg cggaagaaac tccttcgccc ctgcccgcat tgccttcaaa atcacggtcc
     2581 catcgtggct agaactgacc acaaaaatcg cacaatcagg cgactgctgc gtgaccttgg
     2641 agacgaggtc gagggccttt tcttggtcaa aatccaaggc cacgatgccg atctcgggat
     2701 ttgtctgggc tacgacatca gcaaagaatt cgtaacgcga gcattcggcc tccagccaga
     2761 tactgtctaa tcccaacagg agacgtttca gttcctcgcg accggcatca tgaggatcga
     2821 cgatggcaac acgcagcaca tgactcatag tcacccgata ccttagttag aaaccacacg
     2881 tttccccagc gtgtgcccgc ggaagatcgg gctgaggaga acttgtttat cacatgacgc
     2941 ctcaccatca aaaatatggc ttattttccg gggtgaggaa aactattctt gctaattagg
     3001 ttgggcaaaa agaagtgaag aactatcgag atgccgactt cacgggcgag caccgtccgc
     3061 tcgaaggatc ctctcgctta aacccgcacg gaccatcatt ccgtggccat ccctctccta
     3121 ggttcaattg tcataaccaa tttcgccctt aaatccgggc agcggagatg gctccgacgg
     3181 tcgtggctgc ggctttggcg aggaagagtc gttgacaaac cgggccgagc gagtctgtgg
     3241 cacaacccct ggtttgccaa acgtggtcgt ggatgatccc tccgagaccg catacggtga
     3301 agctgcttgc aatcccggga ctccgctgcc atcgttcgat cgtgccaccc ggactgacgc
     3361 agcgtttcct gtctgtgccg actgcgccga aggaccaggt gcaggcgttg gctgtcctgc
     3421 agcggggtta accgcgccat tggtcgccga aggatcgcag cagcgtggga cctcaatata
     3481 acctttcata aaaagttccc aatccgtggg actggccgtt tccgttcccg gtccacacgg
     3541 tggcacttcc tcggcgttca tggcgtcaac cagctcgggc gtgaccagaa tcagcaactc
     3601 cacatcattg ctgcgttcgg ccacacgccg aaaggcagag ccaatgtagg gaagatccga
     3661 gatgacgggc acgccgcggc gttgggcttc cacccgcgtc tccaccaacc cggcgatcgc
     3721 cagcgtctga cccgcttgca tctcgacccc ggtgtcaacc tctcgaacac gcaggccggg
     3781 aaccgtcgtc gagttgatga cgacgcttcg tgaggagtcg atctcactaa cccgcgggcg
     3841 gacctctaaa cgaattcggc cattacccag cacaatcggc acaaagtcca cctgcgtccc
     3901 atatcgcttg tattcaattg agaccgtacc caagctttgc ggcacgatga tgggaaactc
     3961 cccaccgacc tggaagaacg ccggtcgtcc gctgacggtc accagtgtcg gttcggctag
     4021 gactttcatc aagttgtcct gtcgcaaggc ctccaagacc ccaaagaagg cgtcgtttcc
     4081 atcgaaaacg ctgaagaaaa acgtctcgcc gccactcgtc acagccgtgg ctgccgtgcc
     4141 ggcggcaggg ttggccgcgt tagccgccga aatcagacca ctaacgctcg agcggatcac
     4201 attctgaccg gagatttgcg tccagtcgaa ccccaaagca cgcaattttg tccgcgagac
     4261 ctccatcact ttgacgtgga ggaggacctc gtggacccca cccaaggtta ggttattgat
     4321 gacacgcggg taatattcct cggcaatctg gatgattcgg ctaacgtgct cctgctgatc
     4381 gacgtatccc gaaagcaaaa ctccgctggc aatgggaatg accttgatac tgcaattagg
     4441 aaattggctt ttcagtagga ggtccaactc cctagcatcg ccgaaaacga tgacgtcgat
     4501 ggtgtaaatg tttccttttt catcccagag attcacctgg gtcacgcccg cttttttggc
     4561 aaagatttgg atttgtgtcg gagaaagggc tgtgatgtcc agaatatcag gattgttgac
     4621 ctgtgcctgg ggaatccgct tatctagcgt gagaatgcgg cttgtgttaa ccgtcatttc
     4681 ctcccgctga ctaggtccgg taatttgacg aacgacctgg ttgggagacg agggttccgg
     4741 cggtcccggc gcgacctctc ccgtcgccgg aagagcggcc accagggcaa gagttatagc
     4801 cagacctagc aaaagccgtt tccgtccttt aagtctgatc atccgtgatc gtcctcaaaa
     4861 caaggctccg ccaacaagaa tccatcgcgg ccggttttag attgtcggac acgacatcct
     4921 tgtcgcaccc aacaacagcc tcccgcgctg gtgcgcttgc cctctaagaa tacttaacgg
     4981 cttttttgta ttttaacttt actgatcact ccctcgggga ggcggtaatt cttccggcgg
     5041 caactcggga aggggcaaat cgacctgggg aggagcatca ctcatccctt cggcactcac
     5101 aacccgccaa agcgtccccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     5221 gggaaaccgt ttttgccggc aaggatttct tggttttttc gtcttctgtc aggatgtact
     5281 tgtcgtccac tgcaaagacc cgcacgttct ggaggatggt ccgcgtgtaa tctttcggaa
     5341 tccccttatt ggggttggca gggaagtgca caaccacgtc aacccggtcg ctaggacgca
     5401 acatactggc acttccgctg acggcatcca ccttgaccga gacaacccga taacccttgg
     5461 gaatccatgg agcggcgcca gcatccaccg cgcctttggc gagaagctga tcatccaaaa
     5521 tgggagaacc cttgtagatc ttggtctttg tgcggcgacc ttcagcctcc tcggccttaa
     5581 ataatgcccc ctcaggaatc ttcgactttg gccaatcctc gagtttgatc tgctcaatct
     5641 tgacgggatc ccccatggca atgtcttcag ccgccaccac aatcgcctgc atttcaacgg
     5701 ttggggatgc cgtggcctga tccttagtcg ccaaaacctg tttgacgccg atagcagcga
     5761 caagtccgca acctagggca agtagtagca ataggactga ttttgtccgc ataattggca
     5821 aaaacctcag tcaaaatgtg ttttctcaca aatggcttat tggatggctt cccgccgcat
     5881 cgtcgttgtc gcgcgcaatt cggtgtcccc taaaaaaatt ggcgtcggta gccaactgac
     5941 ttgacgaaat ggcaccgata ccgcaaccgt taccggatca ccataacccg caaggtcaat
     6001 cggatcggga ctaacgttaa ctgttgcccc gtgaataccg cctccttcta ggtagctctg
     6061 aacgaccgcg aggacatccg tgtttgttgc gccttgaacg atgccagcgc gggcaccctc
     6121 ccgcgaggcg ttcaccaaca cctgctggac catcaccata cgtccaaact cgatcatccc
     6181 taagacaaga aggaaaaaca gcggtgcaac cagcgcaaac tcgacaacgg ccacccccgc
     6241 ccgaggtgtt cggggactgc accgcaacca acggcgggac cgagcgcgcg cttgtgaggt
     6301 ctctcccatt ccgcgatctt gacaatttgt tagagcagga gacttagaca agctggcgaa
     6361 tctcatagaa cacctctcca tttgccatga ccgcaaccgg tttagaccgg gaaaagcatg
     6421 ccgttgacga gaaagtacac aatggtccca atagcgatcg ggattccgta cggtaatagg
     6481 agcattttgg gttttctttc cgcagcaagg gctgaaagct tttcgggatc gcggacaatt
     6541 aatatctcaa aaaggatagc gaagaactgc cgaatatgtt tgccccaagc tccccgagcc
     6601 gcaaccatgg cgaccgcaag aatcgcccca atcactaccg tcaggacaaa cgcccaaaat
     6661 gtgtgagcca tgccgatcca ggccccgaca ccggctagca gcttgacatc gccggcaccc
     6721 atgccgccga tggcataggc tggcaagagg agccccaaac ccacggctgt gcccagccaa
     6781 ctggagtaca atcccgacca tccgaagaaa tagccgctcg ccgcccaacc gcttaggatc
     6841 agagggaacg tcagccagtt tggcaccttc aacttccgac catcaatcca ggctgccaca
     6901 accaggatga cgctgacaaa ccaacacact gcccaaatca tccacggttg atctgcggtt
     6961 tccgtaagtc gctctaacat agttggttcg gctccatgaa gattgttagg cgaagatcaa
     7021 aactcctgag tgttgtttgt ttacaaacgt gccgtaaaca tgtactgcaa aacggtggcg
     7081 agcaacgtta gccacagggc cagcagcgaa aatgcgtgtc cagcgagttc cgggggcaca
     7141 agcggatcca tggtggttcc tcccattatt tcagccctaa caacatttag ttcgaaaacc
     7201 ctaacggacc gtcatgctct tctaatacaa gtctaggtca aaaaaagccc tcggaatcga
     7261 attttcgatt ccgagggtca ttgaatcccg ccggacagcc cgcttaacac agcggggcta
     7321 agttacccat catcaaccgc caccggctgt gctctggatg ctgtcactga cactctggaa
     7381 ggtgctgttg gcggatgtgc cgagcgagga aatcgctgtc aagcacacaa ccacgatcag
     7441 agccagcatc accgcgtact caacagcggt cgggccatct tccgaaacga ggaaacgttg
     7501 caacttcaaa gcgagcgatc tcatacagtt cctccaagac aagcgggaca tgtttgactg
     7561 gtgtctgcgt ccttccgcaa acccagtcgg aaaaggggga ccgacggtac acgccacata
     7621 cctgtggcgc cggcctgtcc tgtaccgtcc gcatcccaac aaccaaacca aaatgcctct
     7681 tccccgtatg ccgcgatgct cacccggttt tccggatgcg cgccgccgca gccggcatgt
     7741 cccggttgtc ggcaggcgga tgtccaggat caccctggac tgcatcaccc tttcggcagc
     7801 ccggcgaccg ttttgtcacc aacccggccc gtagctttgc gccccggcct tacgaccggt
     7861 ttgccttttt cgaggatgag gcgacaggcc tttctgacag gggactcagt ggccattcca
     7921 ctcgagtctt cacccctgcc gcatgacaag ccattcagcc gtcatctcag cgacttctta
     7981 cttcgacctc tcagccgtcg ttcaaagccg cctctgtaaa gaaccataga acatctgcag
     8041 gccttgtcaa atgaaaaact tgaaccaaaa accgccgttc ccgcgagaaa ttcggccctc
     8101 tttagcgacc ggtattctga cggtatcagt tcgtccggtt gtgccaggga catcccttct
     8161 cgttcgtctc cccggaacgc cacatgcacc aacccgtcaa actgatttag agtctatccc
     8221 aaaaactccc gctctccccc tgggagagga ttggggtgag gggaagcgat cgtcggaatc
     8281 ggttgagaaa accggcagcg attcgagcca ccctcacccc cggcccctct cccgcggaac
     8341 gggcgagggg agatcgtggg ataggttctt agcggctttc gatgcgcctt gcggatagaa
     8401 tgaacgttga gggaatggtt cgggataggt catctcctgt ctctgttgag gtaccgatac
     8461 tttgtggaat tgtgcgcgat gaatgtgatc gaaaaccaaa gcgtcgatca aatcgcacgt
     8521 gaatcaccca cctcattgga ggtcgccaac gaagcgcaac cgccagtcgg ggtgataccc
     8581 tcagagagtc ctacagcgct ccccaccacc ctgctcgtgc gggctgtgtt atggctaatc
     8641 gcctttgtca gcatcatctt ggccgtgccg tatctggccg aagagatttc ctacgccatt
     8701 gtccgcggcc gattgcgggc agaggccgat tcggccaagg cctggctcca acaggcacca
     8761 gaattggaga atcgctatcg ccacgtcatc aagaccgtct tcccaggcgt agtgggaatt
     8821 gaaacggtcc gcgaaaccac cgtggcccgc ctgcctcgca ggccgtttcc cctgccagac
     8881 tttcgcgaag aaagcatggg atccggcgtc atcgtcgata cgcagggtta tattgttacc
     8941 agtctgcacg ttgtggaaca ggctaaagag atcacggttc gcttggcgga cggccgtttg
     9001 atcaaagacc ccacaattgt aggaacggat cccctgaatg atttggccgt cctcaagatt
     9061 ccctccaaag gcctgatggc cgtcccgtgg ggcgacagtg acgaacttga agtcggcgat
     9121 actgtcctgg ccataggcaa cccgtacggt ttgacacgga ccgtgacagc cgggatcgtc
     9181 agtgccaaag aacggcgggc acaaagccag gctggtggct tccaggagtt tctccaaaca
     9241 gatgccgcta tgaaccccgg caacagcggt ggtccactgg tcaacctgcg cggggaaatc
     9301 gtgggaatca attccgccat ctacggcgag gcctaccgcg gcatcagttt cgccattccc
     9361 agtcgtgtgg cacggcacgt gtacgagcaa attcggcggg atgggcaaac gcggcacggt
     9421 tggttgggtg tgcaaatggg cgatgtcgat cagtcaacgg cccaagccca ccgattggat
     9481 aggccacagg gtgccttggt cctctccgtg ttacccggct cacctgcgga agccgcgggc
     9541 attcaacccg gcgacataat catcgcgtgg gacggcaaac ccttggaaga tgccgcggac
     9601 ctcggtgttg ccgtggcaag aacacctgtg ggcagcgagg tgaccgtaga acttcaccgc
     9661 ggacaggagc tgaagaagct caccgtccgt gttggccagc gacctatgtg atagctcgtt
     9721 gggcgatacc tccgtgtctc ctactccgtc atgtgatccg gggacaattt agagtctatc
     9781 ccaaacagtc cgcctctccc tctgggagag ggttggggtg agggcaagcg atcgtcaaag
     9841 tcgatcgaga gaacccgcag cgattcgagc cacgctcacc cccggccctt ctcccgccga
     9901 acggaagagg ggaatttgtg ggacaggctc tcaggctgtg tgggcggcca tcttacgatt
     9961 atttcccccg cgggtagcca cgtcgtagag aatcgggatt acgatttgcg tcaaaatgcc
    10021 cgacacgaga atgccaccca ccgtggccac accgacgccg acccgtggct ccgccccaat
    10081 tccgcgacct agggcaaggg gcagcatacc caatacggcc gccagggtga tcataacgat
    10141 tggccggaac cggtcgcagg cagcctgaat catcgcctga tgccgcggca ctccttgggc
    10201 aatcaggacg ttgaactggt caatgatcaa aatggcattg ttgacgacga ttcccgtcat
    10261 catgacgatg cccatcacgg caaagatgtc caagctttgc ccggcaatgg ccaatgccca
    10321 gaatgtcccg atcagcgcca tgggtacggt cacaacgacc aggaaggtcc gcttgaagga
    10381 ttcgatgatc gccgccagtg acaggaagac caggagcaca gagatgataa aggcctcaaa
    10441 taggccccca agaccctctt gcatgacctc gtagatccct ccgaagctgt aacgatagcc
    10501 agggggaagc tgcacctcac gattcacccg ctcgctgatc agcgtgactg ccttacccaa
    10561 gggtaagtcg ggagcaagtt gtgctgtcac tttggaaata cgctgtttat ctttgcggac
    10621 gatctggatg ggaatctttc gttcctcgat ggtacccagc gtggcgagga tcagggcatg
    10681 accctcaacc cccgggaaca tgaaaccggc tacctgttgt ttaccctctt gttccatgaa
    10741 tttgacgaca atatcgtagt tgcgtgccgt ctcacgcgat ttgaaggtcc ccgccttgat
    10801 gccttcgagg ttcccgcgca acgtcagtcc catgccgacc gctggcgagt tgatgtcggc
    10861 caaaatcggg cggcgtggtc gcacccgaat ctcgggctta ggctcgcgga ccgaggtatc
    10921 cggcgataag acccccggaa tctcccgggc caaccgcaaa ctcgctaacg caagctggtc
    10981 cagcttttga aggtccggac ctgcaatctc catttccacg gggttggatt gaccacccat
    11041 cagcgacgcc tggctaacca caatgcgggc atcaggaaag tcttgaagcc gttgctcgac
    11101 aatgtccatt aactgataga tggtcaattc gcgttctgtc cgctgggaaa aaaccagcag
    11161 catttggcca aggtaaacgc cttcgctgga ctggccgaga acggcctctg ccttaccgat
    11221 ggtggtgagc atgtgcttga gttccggtag gccctcaaga cgccgtttgg cctcgttgat
    11281 cttggcagtg gtggcggcca aatcgtaata ggtggggaat tccaagcgaa cgtaaacctc
    11341 gccgcggtcg gtttccgtaa agaaacccgt ccccaatcgg cctgccaccc aaagggaatg
    11401 gacaaacatc cccacaacgg caagcaagac cagagcaccc accacccgat aatgctcagt
    11461 aaatcccaaa agccaccggt acgccgagac attggcctca aggaaccgat tgaatccttc
    11521 accgatccga cggagaagcg aacgactccg tttttgcggc ttgaggatca gcgagcacag
    11581 catcggtgtc agcgtgaacg aaataaacag cgacaccagc gtcaggataa acatcgtcat
    11641 cgccaaaggt gcgatgaaaa gcccgacgcg ggtcttcatg accgccagcg gaaagagcac
    11701 caccacgttg gtgatggcac tggcaagaac ggccacgaac acttcattgg ctccgattcg
    11761 ggccgcctct ttcgggtctc ccaactcttc aagtcgcttg atgatcgact ccagcaccac
    11821 gatgctattg gtgaccaaaa taccgaccga caacccgatg gcaattaacg tagatgtgtt
    11881 gagggtatat cccacggcct gcatgaagaa gagaccgata ataatcgtca gcggcatcgt
    11941 gatcgagacg ataatgaggg agcgaagttc atacaggaag aaaaagagaa tcgccgctgt
    12001 caggccaata ccgatggcca cgtttttcca agcgtccaag ttcatggctt caataaacgt
    12061 ggcgtcatca gtcacccaaa caagctgcat gcccgacggc aactcgcggt tgattcgctc
    12121 aagagcctgt ttcacggcat tggccaccgc gacggggttg gcgtcggcct ttttgatgac
    12181 cttaatggca atgcactgtt tgtcgtcgag ggtagcaagc tgccggacct cttcggttgc
    12241 cagaactgcc tcgcccacat cacgaagata acagcgtttc ccctcgtgat tggcgatttc
    12301 cagatcgttg aggcccgcca ccgtttcata atcggcatcg aacttgaccg aatactccgt
    12361 ccctgcttcg cggattcggc cagccggaat agtcttaaca ctagcctgca cggccttgac
    12421 gacatccatg gtggtcagcc cgcgcgccgc cagtttttcg cggtccaatc gcagttgaac
    12481 ttcccgcttg gcgccaccga tgagctggac atccgccact ccctgaatca ccgtcaagcg
    12541 atcgcgaagc tcgttgtcgg cgtaatcgta aagttcatcg agcggaacgt cgcccaccag
    12601 ggccatcgtc gccaccggct tggcgttgat gtcaaacttc tgaatgatgg ggtcttcgac
    12661 atcctccggc aattccgacc ggatgaggtc aatcttctca cgcacgtccg tggccgcaat
    12721 atcgacatcc acctccattt cgaattccag ctcggtctgg cacacatttt ccatacagat
    12781 ggaggaaacg tgtttgagac cctcaatgga ggagacggca tcctcaatcc gcttggctac
    12841 gtcgatttcc aattcctccg gtgaggcacc cggataaatc gtcaccacag tgatgtaggg
    12901 aatatcaatc ttgggcatca gctccaagcc catcttgcga aaggcattca ggcccaggag
    12961 cgttaaccca atgatgagac agctcgtggc aacgggacgg cgaacagcaa agtcggcaat
    13021 cttcatcgac ctgcctcctc caagacacga accggcgtgc catcgtcaac cagcgattga
    13081 ccactggtga tcacaggcgc cacagcagac atccccgcac gaatctcctg ccagccctcc
    13141 gtctcccatc ccagttccac cgcaacggcc ttggccttat tgtcttccac aatgaacaca
    13201 atgttcttgc ccgaacgttg ctgcacagcc tgctttggta cgcccaaccc ttggcgtctg
    13261 cccaggatta ggctcacttg agctaaagca ccggggacaa cctctggcgg aggagcttgc
    13321 acgagggctt tcacctgaaa tgtccgcaac cgagattcga tcacaggact cttaaacgtg
    13381 acaggcaact cgcccaaatc acgtcccgcc acccgcgccc gcagtttcgt ctctcccaca
    13441 accacctgat cgtaatactc ggacgggaca aaaatactca cttcaacaag ggaaaggtca
    13501 tcaatgcgta aaacgggcgt gcccgctcca gccatttcgc ctggttcctt catccggagc
    13561 gagacgcgac ccgaaatcgg tgccacgacg agtgaatccc ctaagtcctt ttcggcgatt
    13621 ttcagattca agcgcgcctg ctcaagctgc gccttgctca gctcaataag tgtttggaca
    13681 tgccggacat cggcttgcag ttgacgcacc tgtgcctctt gatgctccac aacctgccgc
    13741 gcaatcgcgt ttcgttgggc aaggtccttg tagcgagcca aatcggcctc ggccacctgt
    13801 tgggcaacga gcgtcttttc caggagggcc tctttctcgc ggaccgcaca ttccgccacg
    13861 gttacctgtt cctgggcgat ggcgaccgcc tgggtcaatt tcacggaatc cgtctgaaac
    13921 aacttcgtct tgcccgcctc tacaaagtcc ccttcatcaa caaagatggc atccaacgtt
    13981 ccgggaatcc gtgccgagac cagggcaaat cgcttagcca agacgttccc cgagacgtca
    14041 agccgatgct cgaagaccat cgtccgtgcc gcttgaacga ccaccggcac cgcatctcgg
    14101 tcccccactt ttgcccctga agacaccgcg gaagtctggg tagcggatgg ggtcttttca
    14161 tcaccaaaca tccagctatg aagcatcgcc aatgcgatcc cgacgcccac cacaagggcg
    14221 gcgaacccca aactgatgat cccctggagc agtttcttcc gattcatggc gtattcccct
    14281 taacacgctc ttgaagtcca acttggcaca tcccgaccct caaatttccc ccacaaggcg
    14341 tctcctcatg ccctgcccca caacaaaaat tgcggcacac cttgtgattg cctcctcgca
    14401 attctcctgc ttctctgcgt ttggccccgc ggttactcgg cagccacttg agaaggctcg
    14461 ctgctttccc cagtcttccc ctcaacctga ggtgcttcac atcgctggcc tattgcagac
    14521 ggactctccg cgctagccga agaggcaccg atcccgtgaa gcacaatatc catcaggcgt
    14581 cgcactgaga cctcaatggg atgggcctca cccgtttcaa gttgtcgctc gatgacaaat
    14641 tgcaaagagc caataatcat ctccgccgcg taggttacat ccaggggacg aaacactcct
    14701 tcgttgatcc cctgccgcaa aatcgcacta aagcgatcga tggccagccc gttcttggac
    14761 cgccgggagg tcaaacaaag ctcgcggacc gtaggatcac gaaaaaggaa atcgaagagc
    14821 gcccggtgct gagaaaaata gtcaaaccac atgcggacca tggcctcaag cttttccaca
    14881 gccgggacca cccgctggaa aatctcttcc cccgcttgga tggccggctc gatcgtctgc
    14941 tcgaagagat aggtcaccaa ctcttgctta ttgcggaagt agtgataaag actccccttg
    15001 gcgatcccgg ccacctctgc cacccggtcc atggtcaagc cctcatatcc gtactgtttg
    15061 aggacctcta gggcggcctg actgatgcca tctttcatgg ccgaaacggc aagctcgcgg
    15121 catttttctg tcaaactggg agccataact tgtccctacc tttactgaca acacctctgt
    15181 tgttagctcc gctcaatcag cggttaacca ccgtcgggga ttcggcgtgt catttaagaa
    15241 cctttcccat aacctcccct cggccgttcg gcgggagagg gatggcccgt gcggatgact
    15301 ccactcgctg ctggttttct caaacaactc cgacgatcgc tcaccctcac cccaaccctc
    15361 tcccggaagg agagggggac tttttgggat ggactctaag ttaacaccgt gaagatcggt
    15421 ccactaacgt gtatcggcgc cgacacccaa gacccaatgg ttcgattctc tgacgttact
    15481 acgcaaagtt tggacgatcg ttcatatatc tatgatcata gtacatcttg ttgaccgcta
    15541 agtcaatcgt gcgaccgtcc tgttttctgg ttaactgtcc agcccggatt taccgaagtt
    15601 actcaatgca actctatgcg cggttttttg ggaaacatcg attgtgtgat cactgccgat
    15661 tcgtccgccg ggcgaaccta actaaccaat ttgcccagtt tcccgttcgt cttgatccac
    15721 agggggaatc gtcatgaatg aaaggacgcg gccgggccgt ctggaagcta ccgattgccg
    15781 aacggctata gcgagaagga atttacccgt cttaagggtg gataaactcc ccattacttc
    15841 gctagcgttc aggtttagct cgttcatctt cctcatcttg cttacagcgc caggatgctc
    15901 gactagtctt cgcgaatggt gggccaatgg cttcaaagtt ggaccgaact attgtcgccc
    15961 ctccgcagag gtgggcgagg cctggatcga cagtgaccaa ccgggggtga aaaccagtgg
    16021 ggacgtcacc tacgcccact ggtggacggt gtttaacgac cccacgctca atgagcttgt
    16081 ttacacggcc tatctggaga atctgccgct ccagatcgca gcctggcgga tcatggaggc
    16141 ccgctaccag ctcggtgttg cccgaggcta cctctggcct caggagcaac aggcgatggg
    16201 agccttctcc cgtaaccaaa taagcaaaga aggcttccct atgtcaaaat tttctgaaat
    16261 tcccggtttt aagatgtctt acgacgactg ggttgcggga ttcagtttgg cttgggaact
    16321 ggatttttgg gggcgattcc gccgcacgat cgaatcggcc gaagcccacg ttcaggctca
    16381 ggaagaagac tataacgcgg ctctcgtcct cctccaggca gaagtggcct cgacctatat
    16441 ccaactgcgc gagattgatg aacgtctgga gcttgcccgc aaaaacgtgg ccctccaaaa
    16501 agacacgttg gctattgcca aggcccgctt tgaacagggt gttgtcagcc agcttgatgt
    16561 tcatcaggct caggccattc tctccaacac agaatcgatg atccccatgt tggaggcgat
    16621 gcgccgggtg tcggagaatc ggctatgcac gttgcttggc caacccccgc aggacttgga
    16681 tcccatcttg gggccatccg gcaaagtccc ggatgtaccc ccagaagtgg tcgtaggcat
    16741 cccggccgac ctgctccgtc gccgtcctga cgtgcgaagg gcggagcgtc tggccgccgc
    16801 ccaatgtgcc cgcatcggca ttgccgaagc cgaattctat ccgcacattg ctctcactgg
    16861 ccaactcgcc tatcaggctg aaaacttcag tgacctattt agtggcagag cgtttacggg
    16921 catggtgggt cccggattcc gctggaacat cctcaattac ggacgaattc gcaacagcgt
    16981 cctgatggaa gatgctcgct tccgtcaagc tatcctcggt tatcaagaga cagtccttcg
    17041 ggcggccgaa gaagccgagg acatgatcac cgcttttctc atggagcagc ggcgactgcg
    17101 tgcattgcag caggccaccg aggccaccgc tcaagcctcc cagcttgcgg ttgctcaata
    17161 tcaacagggc ttgatcgact tccaacgggt gatcgactca cagcgggcct tggtgcagca
    17221 gcaggatgcc ctagctgaat cgcgtgggaa ggttgccctc aatttgatcg gcgtttataa
    17281 ggccctgggc ggtggttggg aaatgcggcg gaacccctct gccgggatcg tcttgacccg
    17341 gttgcagacc aagacgggcg gatcaactca gcccgcaccg gctccgcaac cgacggcacc
    17401 ctcctcctca ggtggaacac ttccaatggc tccgacggat gggcaaaacg tgtcaccgcc
    17461 agccgcaccg gctccaccac ctgagaaggc accggccgaa ttgcctacct cgcccgcacc
    17521 gccccagttg cctccggcac cgacaactcg ctggggtgat caggacgtac caccgatggt
    17581 cgttcctacg gtgggggaac tccccgaaat caatatctcc gggtgaaatc ctctcactga
    17641 cagccggcca ccgcaaggca acccggctca cccgagcaac cttcaatctg gccgtgctcc
    17701 gaatgggtta attacgctac aaggccgaca cgcgctcaga caggcttgga cgtccagcta
    17761 acatcgaccg cggcggtagc ccgaccaatc aagtcccgcg atatctttgg accaccaggg
    17821 tgtttaacca ccgcttgtgg cgataaagct aggaaccact attcttcttc ccggtactga
    17881 tagtcttgag agttgatggt cgtaacgaca cgaccattaa caagttcacg gatctgttca
    17941 attcggaggc ctttcacgta aaagccattc tggggtagga gatagctctt atcgcctaca
    18001 tggagctcat acggttggct cccgaaattg acgatggcca cagttccgtc cgaaaaggtc
    18061 gtccgctgca cgtcgcgact atcggtgagg aactcgtgat tgagcatctc cgtcccggcg
    18121 atgacctcgt ggaggatgca cgtattgcga tacgttctca agaagacttc acggttgttg
    18181 agccaagacc ctttgcggcg atccgcccac agaagcggga tggtgccgta caatacgtta
    18241 aaggcatctt tcttgggggt gatttctggg gccgcttgca gcagaaaatc gctcgaatca
    18301 ccccagtacc aggtcgtgac gacgcagtca tggaagacaa gctcccaaag cggtgcccgt
    18361 tctcgatggc caatccccca tcgctcatat tgactccatg gcggtaactt gttgccccag
    18421 ggactggtga attcctcatc tttgcgcttc ggtggaatga ggtgcccggc gggccaggag
    18481 aagtaggcgt tgcagctcat aaggccctcg aagtaatcga catagggcac cgcccaccag
    18541 ataccatgtt ccccgcccac cacgagcttc agagaacgga cataactcag cagatccaca
    18601 ccgcactgcc gtttttgggc cttggtgagc atgtgcgcgg gatcgtaaca ctcgtaaagg
    18661 tcctcggcgg tggtcacatc aatgaagcgg cccaggaagg gataagtgga caagattttg
    18721 gggatgtccc gctgcgcggt gcgcagccac agcgcgggac agcgcttcat gtactgagtt
    18781 ttcttatcga aggtcagcca ggccttcatc cgctctccgt cggacttgag gacgacattt
    18841 tgcgggacaa gatcgtgaga tcggtcgatc tgatcctcgt tctctacggg caacacatcg
    18901 gtgtagttat cgtattcgct cgtcagatac cccaactcgt tgacctgttt catttggtca
    18961 ggcggcattc gaccttgcac aagtaaccgg cggatgccgg cgtttttcgc ctcctgggca
    19021 aacgataggt tagcatttcc ccacacatca ggggcgccaa aaagacgcct caactcggga
    19081 ttcgctttga gcttttccgc gaacggtaca atgaggccca gttgttgggc atacgtccga
    19141 tagcgcttgg ccagggccac ataactccct tgatcggaga agtgccaaat cagcttgcgg
    19201 ggataagaaa acttgcccat gctgggggac cagccaatcc aaggtaccca cgcggattgg
    19261 ccatctttct ccaccttacg caactccaga taaccgtcgt cactggtctc cgctatgatc
    19321 atgtacccaa tcccagtctt gacgtcgcaa acacccaccc agggcatatc caaccgcgac
    19381 gtgttgaacc accggcgggg gagttgctcc tcggccaccg gatagaggtg accattgcag
    19441 taatcggcaa tcgcaatcgc cccgtcggga ctgtgataaa agaggggcgt ccagaatcgc
    19501 tggttggaaa atgccacgtc gcggtccggc atatcgaccg tcatgaacag atcggccgaa
    19561 tcgcggggaa gccgaagcgt cacggtcccg gcaattggct tgttatcccg ccaaaaaatg
    19621 gtggtttcac aggagagcgt ttcgtcatcg agtgtcttga tgttccgaag gacgttttgc
    19681 cgtttggggt cctcaacaaa cgacgcccaa gtgtggccaa ctcgcttgtc gagcacctcc
    19741 caagcgccgg tttgcggatt gagggttacc ttgagaatcg cattctccag gacccattgg
    19801 ccaccgtcct cgacaatgtg cgtggccggt cccgggggcg tccccccttc ggaagcggga
    19861 gaggctaatg aggatgccca ccagacgaac cccaacacaa ccaacgccgg tccataccaa
    19921 cgcacaaaca tgggtttctg ccgagagcaa gcgcgaaata ccatggcaca cctcacatcg
    19981 cgtcaaggga agcaaccatc gaactcggtg gatcatcagg caggttttgc aagcactaag
    20041 cggccggcaa cacctcggcc aattgtcaca caacgcccgt agggctgaac actgtgatac
    20101 cttatccggt ctgtgtcggc aaccgggtac cacgtttnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnccg tcggccatct tccgcgttcg caaattgctt ggggtcccgg
    20281 ccaccgactc cttgatgcgg cggttcaccc aaacgggata agtccaccgt cggcgggtct
    20341 aaatacatca tgagcgaggt ttcaccccaa gcggcgtgat cacctaaata accttcgtcc
    20401 agggcgagga tgtattcggg cgtcgccagc actgggatac cgaggcaacg ggtcattctc
    20461 gcgccgacct ctcgcaccac gatctgttgt gccgcgccgt aatgaccggt cagaatgata
    20521 acggctttaa atccttgtcg ggcggcctcc atcgtaagtt tttccagcca tggccggagc
    20581 aaaacggagt agatttgatc ctctggttcc atgacgaagg tgtgaggttg attgagacct
    20641 cccaccccac cgaaaagtgg tggcgccacc aggccgcccc cgcgttgcgc tgccctgatg
    20701 cacaacatgt gtgccttgat cgcatccaaa cccaccacgt tgtgcagccc gtgccattcg
    20761 atcgtaccaa tcggtaggaa caacgttccg cacgcattca tgcggtcttg aatttcctgc
    20821 ggtcggagcc tttccaggcg aacctccggc acaccgactt ggaaccccgt cgcggaagcc
    20881 ggggaggttt ctgcatcttt ggcattctca tttgcggcga ggacctagga acctcccgtg
    20941 gctgaggcaa ccatcccgac catggccgcg ccggcgtttt gtacaaacgt gcgtcgtcgt
    21001 aaaacattgg acatcacacc actcccattg cctcggcata agcgaaatga ggggaccgcc
    21061 ccttcgggcg agtgtcaccc cacagaatgt cctgacggcg agtactgtga taccgtgctc
    21121 tgaatccgct gacaactggg acggatcttt cacgattcga agaggaatcg gctcgggcct
    21181 tggccgtcgg gttctttcat agccagtcgg cgaccgcggg ccgtaggagg agcactctga
    21241 agcggctcgg ccgagccttc caacggatcg ccctcccgga tgctggccaa gcgctcgcgg
    21301 attttcgccg tatagtccgg cgagagctca aaccccagga agcgacgtcc cagcttcttg
    21361 gcaacagcca gtgtggtccc acttcccgcg aacgggtcca caacaagttc acctttgcgg
    21421 ctgcacgctc gaataatccg ccccaaaatc tgctccggca tttggcagcc gtggaagccc
    21481 tgccgttccc ggaatgttcc acagacacgc gggacatacc aggtgtcctc ctccgggcgg
    21541 aaaccatcgg gcaagtcctg cggtcgaagg atccacgtgt cgtccggaat gcggcctttg
    21601 ggatcagcgc ggcggtccgc atagaccaac tggcgggcag aagggacacg aatctcatcg
    21661 gcgttaaagg taaaccgttt cgggtcctta acccagtaca acaggtgagc atgcgaccgc
    21721 gtaaacttgt ttttgcaatg cacgccaaat gtgtaatacc agattaccca attgcggagg
    21781 acgagccggt gttcccgctg gatcatcgtt tttagctcgg ccacatattc atcaccgatg
    21841 gccagccaga aggtgccatc gggcttcaag atgcgcgcaa tttcgcccgt ccagcggaga
    21901 caccaatcca gatactcatc atcatcgagt tcatcgcggt aaacgtcgta ctggtaaccg
    21961 atattgaatg gcggatcggc aaagacaaga tcaaccgtac cgtcggggat cttcctcatc
    22021 ccctcgatac agtcaagaca atacacggtg tcgatctcta acgactccat tgtggtcctc
    22081 gcaagacccg caatccctcg agatccgtca tcctgatgct tgcccatggt gttggaagcc
    22141 tgacatgcgc aaaaacgcac gcgccccgaa cccaccgatt atgcgattgt tcatcctacc
    22201 agcgtcaaga agaccaagcc agcggtgtaa caagccgaat gcgcagcaaa gcgaagaatc
    22261 agtcaaatag acttccggta aacaactccg ctggtgcgct gctgaaaacc aaactctcga
    22321 agcatctgca ccattgtcac gtcgctttgg caacactggg cctccacaac gccaatgccc
    22381 tcggcccaaa gcgaggaaaa agactcctcc atgaggaacg aatcggcccc agtgttagcc
    22441 caaccggcgg cggtgcgcac ttctgccagg cccacggccg gtctggggtg aggattagga
    22501 tcaaggcggc aaaatcgaac acgagcaatc ggttgctgat tggccggatt gatcaatgtg
    22561 tactgaatga tttccgattc gcaaaagcaa agggcctcgc accaagagac gggcacagga
    22621 tcggtcgttt gttgaatgtt gaagcctgag aaccggccga tgatattact atcgaggaga
    22681 tggcgatatt gatcccgact cacttgaaaa cggaggtatc cacccacgac ctgatacccc
    22741 cgagctccga aaagcgtcgt aaggagggta tcattctgca tgatccccgg caatcgaaaa
    22801 ccgccgtaaa gaccgagata aaagggatgg cagggatttg gcgacccccc gtaaaggacc
    22861 cgcgcaccac cgcgtgccaa ataatcttcg cagcggtcga gcaattcgtt ggcaaccacc
    22921 tgctgatcac acgccttgga gaccatcaac atgcaggtaa tgccttgctc gcgggcaagt
    22981 tctccctggg tggcctgggg aagaaacccg gcgtgggcaa accccacagg ctgatcgtcc
    23041 tcgaaagcca aaaacaaacc gttcgggtcg aagtacggct tgttgaggac actccgttca
    23101 aggtagggga gggtcaatgg ctgaatcagc cctggcttgc cggcgcactc cgaccagatg
    23161 cgcaacagtt ttggcgggtc atagttccga aaaggtctgt agctagtcat tcgcccaatg
    23221 ttccgaaagg cgcttcccga cgttgtggca acatcgcatg gaatgggtga gggaccaccc
    23281 gaaatttggc tggattgtgt ggatgaactt gccttccctt ttcaatcgtc cactgactcc
    23341 tctcagtcca attttaacag gtggactcgt tgagagacca taccaagccg aatgggtctc
    23401 gacacagttt taccgcagtc gagcaaatcg tggcggttgt tcgccgggac catcactggg
    23461 gaacccccag ggttacccct cactagtttg tagggcagct caggacacag tggacgcgga
    23521 gccacgcgtc cttccagatt tcaaggaaca caacggggaa ggaaacgggg caagcccaag
    23581 cgtcatcctt gtggggaacc gcagaaaccc tatgacagaa acccctgccg ccggccccct
    23641 ctgcgagtca tctcctcaca ccttctcgcg gccgtctata cttaaaagaa cgctggtgga
    23701 tccccatcgc aaaagtctgc cattggcaag gtgctaaggc ctatcccaaa aaagtccccc
    23761 tctccctctg ggaaagggtt ggggtgaggg caagcgattg tcggaatcga tcgagaaaac
    23821 ccgcagcgat tcgagccacc ctaaccccca acccctctgc cgccgaacgg gcgagaggag
    23881 attatgggat aggctctaac taggagcgtg aactggtccg ttctagcatc tgggctttca
    23941 ctacaatcaa ggcaggtgtc ccacgtgctc aaagtcaatc tggttcaact tcctatccca
    24001 ccccttggcc ctgaaatcgt tcgcggcaat gtcccgctcg cctccgctta tctcaagcga
    24061 tatgcccagc gcgtggcagg ggtcgatgtc gagatcgaaa ttctcaccgc cgatctcacc
    24121 gatcgggcag gggatgccgc cctcgtgcgg gagctatccc aacgggggcc caacctcgtc
    24181 ggcttctctt gttatctgtg gaacgttgag cggtcgttat ggctggccga gaggctaaag
    24241 gcactgcatc ccgagcttcg cgtggttatt ggtggtcccg aggtcaccct ggataacacc
    24301 tgggtacttg agcatccggc gattgactgg gccgtggtcg gcgaaggtga agcaactttt
    24361 gccgacctcc tcaaagccct gacagccgag ccgaggtggc ttcaacatcc aacagagctc
    24421 ccacccatcc cgggactttg gaaccgggca actcattcgt tcgggggagc acgttcccaa
    24481 ctgccgactt tgaacgaagt tgggcgggtc tatttggatg gcctccttga cccgcgatgg
    24541 cacgacaccc tgttcttgga aaccatccgc ggctgcatct ttcagtgtgc tttttgctac
    24601 tatcctaaga gttttgaagg ccttcggttc atggacgagg atttggtcct agccaacctc
    24661 caccttgccc accaagcggg gatcagcgag gttttcttgc tggacccgac gctcaaccaa
    24721 cggcgagatt ttctttcatt tcttaagctc ctggctcgcg ggaatcccga aagacggttc
    24781 tcttactccg cggaactgcg tgccgaaggg attcgggccg agcacgccgg gatcatgaag
    24841 gccgccaatt tcgccgaggt ggagatcggt ctccagtccg taagccgcga ggcccagggg
    24901 ctaatgcatc gtcgggtcaa ctatcaggcc tttgaagagg gtgttcgcgc ccttttggag
    24961 gccgggattc gtgtccgggc agacctgatt ataggcctgc cgggagatac ggcggagacg
    25021 attcggcagg gattccgcta tctcctttcg ttgggccttt ctaatgaacc ccaaatcttt
    25081 cgtctttcag tgcttccagg gaccgagctt cgtcgccgga gcaaagaact agggctggat
    25141 taccagccgc atccgccgta ccacgtcctg gggacatcga aactttccat cgtggaaatc
    25201 gcccagttaa tgcgagaggc ggaattcagt tttggtaggc gcttcgaccc ctggcccgtt
    25261 tccgacgaag agttcagtcc cacgatcctt aaagagcgcg gcagcttttg ggaaatcaat
    25321 ctcgatcggc ccgagccaac cgagcttccc gacattcaga tcgcccacac gctgtggata
    25381 aaagcccggc attttgcagg ccgagccaag gacgttcgct actgggctga ccgagtgctc
    25441 aacgagaacc cccatgtcac gctttggatt gtgcttgata tcagcgacgc acaaacctta
    25501 cccgaggagg acgatatcca gagcatcctt cgggcctgct atgaccggga acacagttac
    25561 tgggattggt atttcgcgcc ggaattgcct cctggaccat cgggagcaaa acggttggtg
    25621 gtttacagtg gccggtcact caaaggccga cgccgatggc tcaggcagag gttttctccc
    25681 tcggcggatt tggtcgaagc cccggaatgt caagccaaag gccgcggcag gcgaagaagg
    25741 acttgcgatt caggaaatga atagggtggg catttcgatg gaatcggagc caaaaactca
    25801 aatagtcgag caggttgcca ttgtagtggt gtgccgggat gaccacgtct tggtgggaat
    25861 tcggcctgac ggcgtcccct ttgccgggct tgccgaattt ccgggcggac ggctccaacc
    25921 gggagaatcc tgggaggagg ccgctcggcg ggaaacccta gaggaaaccg gcctctccgt
    25981 tcgcgtcgag gccctcttgg gcaccgtttc cactcgatac gaggaccgta tcctggaact
    26041 gcgttttttc gccgccagcc ctgtatcccc ggcggcctca ccgcgggaac catttcgctg
    26101 gattcgtcgg gacgccctcc gtgccgattg ctttcccccg gccaaccgga ccattattga
    26161 gcagatccaa aaaggacacg gccaatgatc cgggccgcgg ccaagggcct caagacgttc
    26221 cttttcccaa tacttatgtg aaggttcccg ttagcagtga aggatccttg tcttcgggcg
    26281 gcccacctat gttttgcacc caaagcctat cccaaaaagt ccccctctcc caaagggaga
    26341 gggggttttt ttacgatggg ccacgaaata tacgcggcca gcccggtagt tgcagcaccc
    26401 acccggcgcg gatgccatgc atatcgcatc ggacttggat ttcgcacgga cccgagcgct
    26461 ggttgaatca tgggctaggc acgggagcgg aaatttgggc aggcacaggg gcctgcccct
    26521 acggggtttg aatgggtgcg tcaatcgtag gggcaacccc ctgtggttgc cctgtttgtc
    26581 cgcggtacgc gcgatttgcg tttcgccggg tgcgcgggct gctttgactt tgtgcggacc
    26641 cggcagttgt ttgggttctg gggcaggcac aggggcctgc ccctacgggt ttcaatcgga
    26701 tcatcaatcg taggggcaac cccctgtggt tgcccagttt ggccaaaggg acactgggga
    26761 aggtcgccgt cgccggcgac caaatcatcg gacggcggcg accccgtccc taccgt
//
LOCUS       DAOSUT010000043        26788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1191_length_26788_cov_9.457861, whole genome shotgun sequence.
ACCESSION   DAOSUT010000043 DAOSUT010000000
VERSION     DAOSUT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 26788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26788
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_26788_cov_9.457861"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLD05_05645"
     CDS             <1..285
                     /locus_tag="PLD05_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76953.1"
                     /translation="TLANHNAAHIPDPPALGDRHDAYPLGWAIRREGNGLFPPTIVPS
                     DRPPSDPQLVQTLLEQSSFPPGLSRYLLQPGTNGYPDVDTYRDLEFYLEE"
     gene            558..4118
                     /locus_tag="PLD05_05650"
     CDS             558..4118
                     /locus_tag="PLD05_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76954.1"
                     /translation="MPRQRSQRLSLVVNPAKAATSFCRGYGWLAWLAGAALVFAFVVT
                     STTLWALEPAPAAVGGSYLLESPMDLSPIGPAGTSTGSQGTISGENTTPPKPPAPSSP
                     GGVTPGTSQPAAIQEVKPEQIYIRDVDGSLKLMLGWTMAQFEELVRLRDGLEQRNQAP
                     PYVIEEIHVQGKCFENYAELSVRVRVRLRGDQPYKIPLRLEQLIPRELPRRVNGQPLL
                     FSYSRQDGGYTLYVQGTADSVEEIIFQGWAPISHVGSEARLVLSVPEAPISDLELDVP
                     LGDIEPSVTPGAMILGIDPVEGGRRLKVTGLGPGFVLTWMTPPSAEWDTATVLESNGA
                     ISVRVTERMVDFDARLNVRSYGIPFDRFRVTLPIGARPLPFSSTSYTVQEVKREDQAG
                     ASQSDRTTVEVLLAKKTTGPVEVRLTAQCEKEPHAWIDIGGFSVEGAVRQSGYLSVMP
                     SSDQALIWGEWKGVRQVSHIPTAANGEASETVVHLEYFMQPCTVVLKSVPRVAYVSIA
                     PRYRVELTTEQARLQARLNYDVQGADVIALDIDLAGWQLQEALLGEQSTVLAVTGDPK
                     GRATLALPQPMSGKLEITLLATRDRPLENKPLMLSFPQPKAQVVQPAEVLIMAPANLE
                     LRADYKESEGVEQALPQATEKGVSAVNVVSFRLNRPDARFVADWTTHEQDVSVDIVTR
                     VRPEGAQLTVEQQIRYQVRYEPWTGAWRFRVPSGIEQITVLLDGRPVTLTPVAAETPT
                     QSAAESEKPSSGVILDSAGNVFAVLPIGPILGTTELVFRYIVPGGLASPEATRWQIPL
                     VDPLDGRLQQHRLSISASVGWNIESVVAPWQIRETTSLSAVSASQIECETKEHTTQIV
                     LRPRVGTFGETNVVVVERMLLQTRLDRLMRQDRCAIRFSTRQPTLTLELPRGARSDRV
                     QIWFDGRPVQARPTSRTSLIVPLTVTSPGPGTGETSTEHLLELWYELPRFRHSWGRTY
                     LECLRLDPHAIVQRISWEIVLLPDEHIIWNGAEVTSEYRWGWTGYFWGRLPTISEENL
                     ERWAGVAEGWLHVSGQANRYLFGALRAVDGVNIWCLRRPWIVGGCSAAVVFTVLGLVY
                     FGPLARKAVLLGAVVIVTTLVLLRPDVALLAGQASALGLALAILSLVLYRAVSAETAA
                     LRANAWSERTALETVICPRTAEVGENCSPLAAERPTEVHR"
     gene            4129..11787
                     /locus_tag="PLD05_05655"
     CDS             4129..11787
                     /locus_tag="PLD05_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76955.1"
                     /translation="MAKSANHRAYHPTSLWRGHAPWVCWLVIAVSLGTLGFSQEPGEL
                     ETQPTPPATSLPLAEATTDLAPLRFRRVFVPKDVLDQVRDPVPYFPLNAAEFEKMIAA
                     ANRQVVFRGQHARIVTAIYRANVRDHDLVGLEGEWLVELFHGTSAILPLGKLNIGLSD
                     PVWVDLGGAPVSLLSDQEGNVSLYVERSGKVGFRWSAHGQTVGAGGSIGFDLTFPPAI
                     RTELELYTSDRFDVAAEGGLVFPMSSSSGGLAGYRLIISDPSRFGMVLQPRRPPAQPV
                     PRGDYREEIAYELTLQGLEATWQWSIDVVDSPLEGLKIRVPQHVRIMEISLGDVALPW
                     EYIQRNDSLTDTIRVRFPEPLTGHNRRVTLRGLTSLQLDGPIVLPTLALEGLFWRSGV
                     STVIVRMPLAIQDVKLRSARLRDVRTLSAGPLGTVLEVVHDTSGADMTLFCRLTAPKV
                     DETTVVVTKITPSEVQAEVRSALRSDGDGCFELTWPISPAWTILDVSSSDQSLLAGWQ
                     FRPGGDKPSKGGALTVRLKKSLPTDKPALLRMRLRRDPPTWDHAVLFDEMIPLVASAM
                     PSTAHLYAALILDPAMAAGRTIEVEPDVEPIGPVELTSLANLYETGSSLQLFDLSELD
                     VKPKVRLVPRQFRYQAQINGKIEINGENLLQSFRVTIEPDQTGVNELCVFWSEVGVDR
                     QFDWFLIESEGRETPLSVSEQSFGPCHNIKTSVVSLPKTMQGPFVVKAQRRTALQPGP
                     IPLLLLPNATHYEALITIIGDARSQSGPTAFGEWRASGLQLVDRTAALSETGQPTVAS
                     YRYWGTADGGPASIFRLELVSHETGIPGGTWASRGIVAIRLDASGLLERWHIFEMKGT
                     LQEELVFRRSVEEPLPEKMIVLVDEEQASFQTASDEKGPSLRIHIPSGRQPQRLTIWE
                     RFSRPSFFIVCPLRVRQLEPAFPVKEWQTSVWLPPVYEALRVSEFHDWGVYWQDWAVR
                     LFGPLKRPPDQPLFLPDRINRMIFDPSLPPPNLAMRRAIQFQEELGKHLQELAEITNT
                     SGLQSQRQTLSPASASVAEDLPAGSAMPKSTPAATKTGKSILWRDLLSDSFLDRVHNL
                     PPGIRTVAFLVDLAALKDLGIQPVDPIPLDDLGDMGQFTDPALKAEKFLTHHSLTIVV
                     TQSAVLLTSLATTASHRNEVQWTNPPIYKLDSHSQWWQEIERAVAAGDGRRYCRIRLW
                     NDLPATVQPTTNAGADPGIAFAAQGWNATAVPQAELEGNEFVTLMIFRNDTLQATRWL
                     GLLVAFFAAWLGGFQRRFRIVTASLVFAVVVLFVPPPFVPLASGFFLGSLLALAASLL
                     VGSSDVKGKGQPIDQGPSPQPQTSGGPLPAEPVPTNLGKPQPSGTSLISVGIVAIFLC
                     CFGDAKSFATSPEAPEPPPTLLSQNGSAVSHPAEDRGPSRGSTLGALSLIAQTTQLPG
                     RESSATTPPGVTAPGSATLPPPYRVFIPVDETGKPVGQEVYVPEGLYKELQKRTSPTT
                     AKPSGWHFGSATYRGTLAATPAGDRWELSQLTANFEVWVVDAPTQLQIPLGSAEAVPQ
                     LTDILLDGRPIVATWDQSRLQVNLPESGRYRIQVPIALTPRIDAGTSEVRLRIPSVAN
                     TRLELAAPFAVNRIEVPSARGRVESDPESGRWTAELGAISELVIRWPASSDPLSDTGP
                     QADILYRLRLGPSQATWELRWKFHLNNAQIDQLQVLCEPSLRLDQASADSQVRIETLV
                     ADSDRQILQMGFDSPLKDEGTLMAVFTEPLPTGSGGLRLPDVQLMGIRIARRTLFIQA
                     HPGVSLDVRESSGWRLADLVTESKRWGIPPGEVGEVYEYLGGPMFWGAWVQPQPVRPT
                     MDEHVQLLATQDEILMRWQARFTSSGGISGCHRVRIPEGFSVRKVAVITGATEQPYPW
                     SVSDDQTLLLLTDRLGPDSQLLIEGRLSLPLSEDWHLQRPTLADAEVQNLTVMLFRAP
                     NVFVEVTKHVGLELVSSVQQPTGDQQLGVFLNAYAARQPQAVDLTLRIQANQPQITAE
                     QFIRVTEAGNRLQAEIRLDVTIINGFLQELKLDLPPQWQIGPAGGERTASPLELKTEW
                     GLTNAGPAGYETGRVKKRLTFAAPQKDTASVALLGSPTLDDHGCVPIPWIGLSEVSSL
                     RSYLIVPKRLEWPSDLANIYGLRPVPQEELKGINIKADETAYELTQTMILPHIVGKPS
                     PDARWEDAEIRIQVSPEGHILGLLTVWFTSSPAEGYQIQMPKGIVPVNAWLDHRCVPV
                     YQRPDGTTHIATDTVKDHRLDLLFAGSVARRGQSLSLALPQLKNAEKAESGRVYLLLQ
                     GASSWNPSLAPGEPVTAWDYHLTRAREAAERLLQLSEGAAGPAPPQGTFGELLKQYLL
                     ARDEARRALLLAPQTAAISYAEVRLQNLELQVSDLLQSAVGIEATLASPGAIAGETLG
                     DALPSSDMQSWYFECEDLPTQIRLQQTVSAQKWGDLLIGSVVIFAVGSIGWLIRRINR
                     PLSVWMMRFAHPLAVLFGIGWWLWLRPSFVGWLLILAALILLIRSRWQWVPKEDAIVP
                     LIVQQKLPTTGSPR"
     gene            12145..12873
                     /locus_tag="PLD05_05660"
     CDS             12145..12873
                     /locus_tag="PLD05_05660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOP76956.1"
                     /translation="MSRKVYIFQNARGEPPGQIVDYLDWVGVRFELIPLFHIRESVSP
                     LDWREVCGLVVLGGPMNVDEVEAYPFLMWERLQLKEAVRREIPVLGICLGAQLLARAL
                     GAKVYPNPVREIGWYRVTLTEGCQSDPLFAGASSPATVFQWHGDTFHLPEGAVLLATG
                     SQCRHQAFRYGNNAWGLQFHVEVTPMLIRSWMELATQNNELAEIGLSASQIEQETPHR
                     FGEMQRLARIVLKRFAEQCRQCSD"
     gene            13279..14331
                     /locus_tag="PLD05_05665"
     CDS             13279..14331
                     /locus_tag="PLD05_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP76957.1"
                     /translation="MLTSRALIEMALAGQPTPRPATGPLAVHFCAKLSGISLRQYTLD
                     PKQLARSVIEYYRRFRPDAVWLSADTWVNAEAMGARVGFPDDDQPAAGLGPALIQSPE
                     DLDAIPPPDVTRHGRWPAMLEAVRLVRQQLGDDVCLVACFDQYPFSLASQLLGLNRMM
                     LAIFDDRPFVERVMEKCLEYTLAYAVALAEAGADILSGGDSPAGLIGPRSYREVAQPL
                     EKRLIERLKERVRVPVSLHICGNATPLLADMAATGADILEIDYPVAIEDAAQILGSSV
                     ALWGNLDPVGLLAQADADTVYRETQALLRRVRQCGIRRFVVSSGCTLAMETPEENLDA
                     FFAAARDFGPWGDKNG"
     gene            14415..15512
                     /locus_tag="PLD05_05670"
     CDS             14415..15512
                     /locus_tag="PLD05_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP76958.1"
                     /translation="MATSVFGVLVHGAGWVSSQHIAAYQKNPATEIRAICDRSLERAQ
                     ARAKSAGLTNVAIYDDFEKALRHPGIDIVSICTPQHVHCENVLAAAAAGKHMVIEKPV
                     AINLTQLRMMQQAVKKAGVRTVVSFVLRWNPLFRTLKALLADGALGQPYYVETDYLSY
                     NGSWWSGWNDARTIEQGVSALLVGGCHAVDALRWFASTEQFKAADAEEVFAVSGGRRK
                     GQTREYNPLTNTWTDNAPPMEYDGLEVVLVKFTNGVVGKVSANAECIMPYRFPIRIFG
                     DRGTAFDNRVWSHKFPDQTDWVTIPTIAPDSSDVTHHPFQAEIDHFVECLQKGTESHC
                     NLDDAAKTHEIVFAALECYRQRRPIRLPLAE"
     gene            complement(16890..18170)
                     /locus_tag="PLD05_05675"
     CDS             complement(16890..18170)
                     /locus_tag="PLD05_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76959.1"
                     /translation="MVTRDGVLIVVFVQCLTWGYAAGTQSAELGPRAGDVYKEYILHN
                     SGDRAWRVTSPEARDSGAQKFLPNPIWELEIDDLEGAIRAEVMLDRWGGHLDTTNPAI
                     RFNKQEWLSLAPPSMGNGVSLPENYYFQDNPVIAVPLEHLKEGKNIIEGICGHKKPGG
                     WGQWGLYSLMLRVYFDPTKKPHLKGRIVSPLSQQTIEENPVITIAVDKEDQLDRIEVF
                     AWYEGYDENGDGRFLDWHGGWYQPNRNEPAEWRDHVGTVLKAPLQVTWNTRWVPDQPP
                     GQIRLIARIRDKSGTWSVTEEVTGLTLHRPNETVTLHCVRDFPPEFGVRVGQRRSCSI
                     RLPESYNPEAVVEAGLHYRTWHGWDKHHAPYKLNDYERPHEGNNHHYHYHIHSIPPQV
                     LRPGDNIFEIFSETDHHMLEILWPGPALIIRAKK"
     gene            complement(18251..19570)
                     /locus_tag="PLD05_05680"
     CDS             complement(18251..19570)
                     /locus_tag="PLD05_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP76960.1"
                     /translation="MAPEAGDDAKIFIWYGDGEGSFRKTIAWHGLAIHEGRAGDFNGD
                     GRPDILVKPYSHRAPRIDVLLNMGTQDLPLDRWTRVEVGQLRHRAVFVNAADINGDGL
                     LDIVAGAWWWENPGAVGTPWRQHSIGLPLRNMATVYDFDGDTLPDVLGTKGEGSEANR
                     EFVWVRNTGRGEFEILHNVHYSGGGDFLQGCVVGFLPNGLSVFLSWHRDGGGIHILSV
                     PEAPANSPWSSQLISQTVSTPPQGEDLALGDIDRDGDLDLLLGDKWLRNDNGQWTTVV
                     LGHIEQGEPDRCRLADVNRDGRLDAVVSLEKGTDVLWFEQPVDPMSVWHKHRVGVVDG
                     QGFSMDVADFDGDGYPDVVVGEHRGFKVNRVIVFRNPGRLQPRNWSDWPAVVIDEGSS
                     GTIDHHDGTLAYDIDRDGDLDIVSIGWHNPKVWLFVNKAIRKTSNAE"
     gene            20198..21334
                     /locus_tag="PLD05_05685"
     CDS             20198..21334
                     /locus_tag="PLD05_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOP76961.1"
                     /translation="MNARKLRKLVVDRLKEAGFLISANGTIHLPLVDKESLRRMHKPA
                     TQLEIARRRDWLLRHLSEYLCFFADGDEIVPERITPALVEVKTDEQHNLFRVARLLWS
                     IPFSKGYGRRLRFLVVDQANGKLIGLLALQSPPLSFPARDRLFCYPPGKKTELVNQTM
                     DIQTLGAVPPYARLLGGKLVALAAASNEVRQTYRRKYSGRATEMEKRTLPPHLVALTT
                     TSAFARSSLYNRLTYKGMRIAESLGYTEGYGTFHLMELYQLFREFLEGQGISTRGGFG
                     TGPRQKWQTMVRALERLGFSSELLRHGIKREVFLFRLIDNLENYMEGRDTEPVYRDLP
                     FSDLVAWWRERWLLPRAVRVDGWREWRKEEIKSLLLIEQEKATK"
     gene            21331..22482
                     /locus_tag="PLD05_05690"
     CDS             21331..22482
                     /locus_tag="PLD05_05690"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOP76962.1"
                     /translation="MMEPVERPFGDLPAALVDEVLKRGEDLSQKLLSDFEQVRTRRQE
                     WRESLKGARLLRRDAELRYVPIPTTCGVDGSYAVERLLASDLVAAAAVAVEGLTPPSE
                     TRHWPEPRHRVWVEIEAHETETGTILRALMIGLELELASQAPHEVVFLDGSLTTALIY
                     FNQALNKSRDTSHLRTRNHLLEQTPSFLEAYSTILTSTRSDRCWVAVPKYTTKREIGQ
                     EMGWPETHDDRGLLSFLLEPGEFTSPRSLQQPSEPWHLNLDPLDCASRKATDHIVEKI
                     IASLGEIQVVYYRPFPWLPALRVEMSRAVAENPARLATVLHAIRHQCGTSAVLEPYPL
                     YMADRMVKHLPKAIPTFRQLTSQYIAETYHGDIGEVFLGLHGYRTESGV"
     gene            22565..24301
                     /locus_tag="PLD05_05695"
     CDS             22565..24301
                     /locus_tag="PLD05_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOP76963.1"
                     /translation="MALDILAGAVNKKLVGELALFRYHQDGLSHYALGQITEVKLRNV
                     WHEDPTMRSLIRQRGRLDTVSERQDTHLGEMIISAVFSAGSNGYRPSILGTVPATGTP
                     VHLVNNDVLSELLSDYHKQLFYLGHVYGSKPVRLPLWFKHFDTGPEGAGEAYHLGIFG
                     KTGSGKSVLAKMILLAYARYGGMAILVIDPQGEFAKEIRGEAVPGGFQLPMKKVLEKL
                     GKPVRVHGVKDLVLDRWDLFAQILYESPFFEQLSIPKGENRQLAAETLTDRLQRAKVT
                     LKDLHARESFDRAWQLLGDENVQKVFYRTEGSRARFRSMYDQADPHFFFNTYWLPVTQ
                     LFREDRPGAQKVEGLLASLFNPVQTQRPLVVIDLSVEQATQASSKVENLPLFGGDQSQ
                     SQGNALFWNETIQSLVIKRLLEGVRIAAEYAYKQNRSLNTLVLMDEAHRLAPREDPES
                     DEKRAVRGILIDAARTTRKYGVGWMFVSQTLSSLHREILEQLRIFFFGFGLGMGAEFK
                     ALSELVGGRSKALDLYQLFRDPHSSFDIASREYSFMTIGPVSPLSFAGTPLFFNAFNS
                     VAEFLKANSLNE"
     gene            complement(25385..26224)
                     /locus_tag="PLD05_05700"
     CDS             complement(25385..26224)
                     /locus_tag="PLD05_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76964.1"
                     /translation="MMVQGHLLEFDRQTIHFYSNTLQAIAAGYLVASVFLFHLPVWGQ
                     VAGTAVLLVGYWLLMLLVPFRGHPAGTLEEKVNLALYIDEQVLGPFRDGTTYTWILSS
                     MAFAGMVLMGVFAGHLLRSRWSRGMKVVWLVLWGVGCLAVAWLWSGGFDQWFQMTLVG
                     NWRFPIIKHLFTSSMVLWAAGWSYLLLALFYLVIDVLGHRWVGWFFEILGANAITAYV
                     AWNLVSFPTIAERFVGGLARYLATFGAPLANVGEALNPVGAFLILWLLLFFMYRKGSF
                     VRV"
     gene            complement(26291..26560)
                     /locus_tag="PLD05_05705"
     CDS             complement(26291..26560)
                     /locus_tag="PLD05_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76965.1"
                     /translation="MHSSTPAAPTTGRIAAIDALRGFDMFWIAGGREAFLAILAIFVS
                     PLPDWFKAQIEHARWEGFTAWDLIMPLFLFVVGLVMPLAFARRIE"
BASE COUNT         5799 a         7536 c         7477 g         5976 t
ORIGIN      
        1 acgctggcca atcacaacgc tgcgcacatc cccgatccgc cggccttagg agatcggcac
       61 gacgcttatc cgctgggctg ggcgattcgg cgagaaggaa atgggctgtt ccccccaaca
      121 atcgttccca gcgatagacc accttccgat ccacagctcg tgcaaaccct gcttgagcag
      181 tcctcttttc caccgggatt atcgcgttac ttgttacaac ccggcacgaa tggctacccg
      241 gatgtggaca cttaccgcga tctggagttt tatctggaag agtaaaccgt tggcaaggca
      301 cgatccatct tctcttgaga ttttcgacgc cacgtatcat tgttcgatca cgaataggta
      361 acggagcacc tattctagag cgattgcgtg ctgccacaat gacgacacat tccgtcaaaa
      421 gacttccttt gcggaaagtc tttggattag gtaaactaaa tggaaggtcc cgcctcttct
      481 gataaggtga aattttgggc ttgtcattga cattgtcatt ttagctggga accgaggaag
      541 gacttcacga cttggtggtg ccgaggcaac gcagtcagcg actcagcctg gttgttaacc
      601 ctgcaaaggc tgccacgtct ttttgccggg gctatggatg gcttgcatgg cttgcagggg
      661 ccgccctagt ttttgccttt gtggtgacat ccacgacgct ttgggcgctg gagccggctc
      721 cggctgcggt ggggggaagc taccttctag agtctccgat ggatctttcg cctattggcc
      781 cggccgggac atccactggt agccagggaa caatctcggg agagaatacg acgcccccca
      841 agcctccggc accaagttca ccgggaggag tgacccctgg aacatcccag cccgctgcca
      901 ttcaagaggt caagcccgag caaatctaca tccgcgacgt tgacggtagt ctgaaactca
      961 tgcttgggtg gaccatggcc caatttgagg aattggtgcg tttgcgggat ggactagagc
     1021 agcggaatca ggcgccccct tacgttatcg aagagattca cgttcaaggg aagtgctttg
     1081 aaaattatgc cgagttatcg gttcgagtgc gggttcgcct gcgcggggat cagccgtaca
     1141 aaatccccct cagactggag caactcatcc ctcgcgagct tcctcggcgg gtcaatggcc
     1201 agcccttgtt gttttcctac agccgtcagg acggaggtta caccttatat gttcaaggga
     1261 cggcagattc ggtggaagaa atcatattcc agggatgggc acccatctct catgtaggga
     1321 gcgaggcacg actcgtcctc agtgtgcccg aggcaccgat ctccgatttg gaactcgatg
     1381 ttccgctggg tgacattgaa cccagcgtta ccccgggggc gatgatcttg ggcatcgatc
     1441 cggtggaagg tggtcgccgc ctaaaagtga caggacttgg gccgggtttt gtcctaacct
     1501 ggatgactcc ccctagcgcc gagtgggata ccgctacggt cttagaaagc aacggggcta
     1561 tttctgtgcg agtgacggag cgcatggtgg atttcgatgc acgcctcaac gtgcggagtt
     1621 atggaatacc gttcgaccgt tttcgcgtaa cgctgccgat tggtgcccga cctctacctt
     1681 tttcttcgac gagttacacg gtccaagagg ttaaacgaga agatcaggcg ggagcgtcgc
     1741 aatcagatcg caccacggtt gaggtcctcc tggccaagaa aacgactggc ccggtcgagg
     1801 tccgcttaac cgcccaatgc gagaaggaac cccacgcgtg gatcgacatt ggagggttct
     1861 ccgtggaagg cgccgtccgc cagtccggct atttgtccgt catgccttcc agtgaccaag
     1921 ccctgatctg gggcgagtgg aagggggttc ggcaggtttc tcacattccg accgcagcaa
     1981 atggagaggc ctccgagacc gtcgtacacc ttgagtattt tatgcagccg tgcacagttg
     2041 tcctgaagag tgtccctcgg gtggcctatg tgagcattgc cccgcggtat cgtgtggaac
     2101 tgacgaccga acaggcacgg cttcaggcaa ggctgaacta tgatgtccag ggggcagacg
     2161 ttatcgcgct ggacatcgac cttgcaggct ggcagttgca ggaagccctt ttgggcgagc
     2221 agagcaccgt attggccgtg acgggtgatc ccaaaggacg cgcgaccctg gctttgccgc
     2281 agcctatgtc tggtaaactc gaaatcaccc tcctcgctac tcgagacaga cctttggaaa
     2341 acaagccgct tatgctgtct tttccgcaac ccaaggccca ggtcgttcag cccgccgaag
     2401 tcctcatcat ggccccggcc aatctggagt tgcgggcaga ttacaaggaa tcggaaggag
     2461 tggaacaggc gcttcctcag gcaactgaaa agggagtgtc cgccgtcaat gttgtctcat
     2521 tccgcctcaa tagaccggac gctcgcttcg tcgccgattg gacgactcat gagcaagacg
     2581 tctccgtgga catcgtcacc cgagtcaggc cagaaggcgc tcaattgact gttgagcaac
     2641 agatacgcta tcaggtccgt tacgaaccct ggacaggtgc ttggcggttt cgcgtcccct
     2701 cagggatcga gcagataact gttcttttgg acggtcggcc ggtcacttta acgccggtcg
     2761 cagcggaaac cccgacacaa tcggcggcgg aatcagagaa gccatcttca ggcgttattt
     2821 tagactccgc tggcaacgtt ttcgcggtgt taccgattgg acctattctt ggcacaacgg
     2881 agttggtatt tcgctacata gtccctggtg gattggcgtc ccccgaagca acacgctggc
     2941 aaattcccct ggtcgatccg ctggacgggc ggcttcaaca gcatcgattg tcaatttcgg
     3001 cttccgtcgg ttggaacatc gaaagtgtgg tcgcaccgtg gcaaattcgc gagacaacgt
     3061 cgttaagtgc ggtttctgcc tcgcaaatcg agtgtgaaac caaagagcat acgacacaga
     3121 ttgtcctacg tcctcgggta gggacattcg gggagacaaa cgtggtggtc gtggaacgga
     3181 tgctcctcca gacacggttg gaccgtctga tgcgtcaaga ccgctgcgcg atcagattct
     3241 ccaccaggca gcccactttg acattagaat taccacgcgg ggcccggagc gaccgcgtgc
     3301 aaatttggtt tgatggccgc ccggtccagg cacggccaac aagtcggaca agccttatcg
     3361 tgccacttac ggtcactagt ccgggtccag gaaccggcga aacgagtacc gagcatctcc
     3421 tggaattgtg gtatgaattg ccacgattcc gccacagttg gggacggacc tatttggagt
     3481 gcttacggct cgatccccat gcgattgtgc agcgcatttc gtgggaaatc gtccttcttc
     3541 cggatgaaca catcatttgg aacggtgccg aggtcacttc ggagtaccgg tggggctgga
     3601 cgggttactt ctgggggcgt ctccccacga tcagcgagga gaaccttgag cgctgggccg
     3661 gtgtcgccga gggctggctg cacgtgtcag gccaggcgaa tcgataccta tttggtgcac
     3721 tgcgtgccgt tgatggggtc aatatttggt gtttacgtcg gccttggatc gtgggaggtt
     3781 gctccgcggc tgtggtcttt accgtcttgg ggcttgttta tttcgggccg cttgcccgaa
     3841 aggcagtcct cttgggagcg gtggtcatcg tgacgaccct ggtccttctt cgcccggatg
     3901 tggcactcct ggccggtcaa gcctccgcct tggggctagc cttggcgatt ctctcgctgg
     3961 tgctttatcg ggcagtgtcg gccgagacgg cggcgctacg cgccaatgcc tggagtgagc
     4021 ggacggccct cgagacagtc atttgtccga ggacggcaga agttggggag aattgttcgc
     4081 cccttgcggc ggaacgcccc acggaggttc atcggtaggg atgtgaagat ggccaagtcc
     4141 gcaaaccacc gagcgtacca tcccacttcc ctttggcggg gccacgctcc ttgggtgtgc
     4201 tggctcgtca tcgctgtttc ccttggcacg ttaggatttt cacaagagcc gggggaattg
     4261 gagacccagc caacaccgcc tgccacgagc ttgcctttag cagaagcaac aaccgatctc
     4321 gcgcccctgc gattccgtcg cgtgtttgta cccaaggacg tccttgacca agttcgtgat
     4381 ccggtcccgt attttcctct caatgcggcg gaattcgaga aaatgattgc tgcggccaac
     4441 cggcaggtgg tctttcgcgg acaacatgcc cgcatcgtga cggctatcta tcgggcaaac
     4501 gttcgtgacc atgacctggt cggcttggaa ggcgagtggt tggtggaatt gtttcacggc
     4561 acctcggcga ttctccccct tggcaagctc aatatcggcc taagcgatcc cgtctgggtt
     4621 gatctcgggg gtgccccggt ctccttgttg tccgatcagg aaggcaacgt cagtctctat
     4681 gtggaaaggt cgggaaaggt tggttttcgt tggtcggcgc acgggcaaac ggtgggggct
     4741 gggggttcca ttggctttga cttgacgttc cccccggcga ttcgcaccga gctggaactc
     4801 tacacatcag atcgtttcga tgttgcggcg gaaggcggcc tcgtgtttcc catgtcttcc
     4861 tcctccggcg gattggccgg ttaccggcta attatcagcg atccatcacg cttcgggatg
     4921 gtcctccagc cgcggcgtcc cccggcacag cctgtacctc gaggagacta tcgggaagaa
     4981 atcgcctacg agttgacctt acaaggtctc gaagctacgt ggcagtggtc aattgacgtc
     5041 gttgacagtc ccttggaggg tttgaaaatt cgtgttccgc aacacgtccg catcatggaa
     5101 atttccctgg gagatgtggc cctgccgtgg gaatatatcc aaaggaatga ctcgctaacg
     5161 gatacgatcc gcgttcggtt ccccgaacca ttgaccggtc ataatcggcg ggtcacattg
     5221 cgaggtctga ccagcctaca actggatggc ccgattgtgc ttcccacgtt ggctctcgag
     5281 ggattattct ggcgatcggg ggtgtccacg gttattgtga ggatgcccct ggccattcag
     5341 gacgtgaaac ttcgctccgc tcgcctaaga gatgttagga ccttatcggc tggaccgctg
     5401 gggactgtac tggaagtcgt tcatgatact tcgggtgcgg atatgacgct tttctgcagg
     5461 cttaccgccc ccaaggtaga tgagacaact gtggttgtaa ccaaaatcac cccctcagaa
     5521 gtccaagctg aagtccgatc cgccttgcgg tctgacgggg acggttgctt tgagctgacg
     5581 tggccgattt cgccagcatg gacaatcctt gatgtctcgt catccgatca gtccttgctt
     5641 gcgggctggc aattccgtcc agggggtgat aaaccgagca agggtggtgc actcacggtg
     5701 cggctcaaga aatcccttcc tacagacaag ccagccctgt tgaggatgcg actccgtcgt
     5761 gatccgccca cgtgggacca cgctgtacta ttcgacgaga tgattcccct ggtcgcttca
     5821 gctatgccgt caacggcaca cctttacgct gcgctgatcc tcgaccctgc catggcagcc
     5881 ggcaggacca tcgaagttga acctgatgta gaacccattg ggccggtgga attaacctcg
     5941 ttagcaaatc tttacgagac gggttcctca ctccagttgt tcgatctttc ggagttggat
     6001 gtaaaaccca aggtacggct tgtcccgagg caattccgct atcaagctca gatcaacggc
     6061 aaaatagaga tcaatggcga gaaccttctt cagtcatttc gcgtgacgat tgaaccagat
     6121 caaactggcg ttaatgagtt atgtgtcttc tggagcgagg tgggggtgga ccgccagttc
     6181 gattggttcc tcatcgaaag tgaggggcgc gaaacgccgc tctccgtgag cgaacagtct
     6241 ttcggcccgt gtcacaacat aaagaccagc gtggtttctt tgccaaaaac catgcaagga
     6301 ccttttgtgg tcaaggctca acgccgcact gccctgcaac ctggtccgat tcccttgttg
     6361 cttttaccta acgcgacgca ttatgaggcc ctcatcacca tcattggcga cgcgcgctcg
     6421 caatcggggc caaccgcttt cggggaatgg cgagcgagtg ggctacaact ggtggatcga
     6481 accgcggctc tcagtgaaac ggggcaaccg accgtcgcat catatcgcta ctggggtact
     6541 gcggacggcg gcccggcgag cattttccgg ctggagttgg tctcgcacga aaccgggatt
     6601 ccaggaggga catgggcctc tcgcgggatc gttgcgatcc gacttgacgc gtcggggctg
     6661 ctggaacgat ggcacatttt cgagatgaaa gggactttgc aggaagaact ggtttttcgc
     6721 cgctcagtcg aggaaccgct gcctgagaaa atgattgtcc ttgttgacga agagcaagca
     6781 tcatttcaga cagcaagcga cgagaaaggc ccatcgttgc ggatacatat tccatctggt
     6841 cgccagccgc agcgattgac aatctgggaa cgcttttccc gaccatcctt tttcattgtg
     6901 tgcccgctgc gagttcggca actcgagccg gcatttccgg tcaaggagtg gcagacgagt
     6961 gtctggcttc ctcccgtcta tgaagctctg agggtgagtg agttccacga ttggggcgtc
     7021 tattggcagg actgggcagt tcgccttttt ggtcccttga aacgtccgcc ggatcagcca
     7081 ctgtttcttc ctgatcgtat caaccgaatg atctttgatc cctccctccc gccgcccaat
     7141 ctagccatga ggcgggccat ccaattccaa gaagagctcg gaaaacacct gcaagagctc
     7201 gcagagataa caaacaccag cggtttacag agccaacggc aaacgttgtc gccagccagt
     7261 gcctcggtcg ccgaggattt gcccgcggga agtgcaatgc cgaaatcgac acctgccgcg
     7321 actaagacgg gtaagagcat cctgtggcgt gacttgctat cggatagctt cctcgatcgc
     7381 gtccataacc tcccgccggg aattaggacg gtggccttct tggtcgattt ggctgccctg
     7441 aaggacctgg gaattcaacc ggttgatccc attcccctgg atgatttggg tgatatgggc
     7501 cagtttactg accctgcatt gaaggccgag aaattcctta cgcaccacag tttgaccatc
     7561 gtcgtcacgc aaagcgccgt cttgctgaca tccctcgcca caacggcgag ccaccgaaat
     7621 gaagtccagt ggaccaatcc gcccatttat aaactcgatt cccacagtca atggtggcag
     7681 gaaatcgaac gggccgtggc agccggtgat ggccgtcgct attgtcggat acgcctttgg
     7741 aatgacctac cggctacggt ccagcccacg accaatgccg gtgccgaccc tggcatcgcc
     7801 tttgccgcac aaggttggaa tgccacagcg gtcccacaag ctgagctgga ggggaatgag
     7861 tttgtcacat tgatgatctt tcgcaatgac actttacaag ccacgcgctg gttgggctta
     7921 ctcgtcgcct ttttcgccgc ctggctaggc ggttttcaac ggcgctttcg cattgtgacg
     7981 gccagccttg ttttcgcagt cgtggtcctc tttgtcccac cgccttttgt tcccctcgct
     8041 tcagggttct ttttagggag tctcctggcc ttggccgcat cgcttttggt cgggtcttcc
     8101 gatgtcaagg ggaaaggaca accgattgac caagggcctt cgccccagcc gcaaacaagc
     8161 ggtggtccct tacccgcaga acccgtacca acgaatttag gaaagcccca gccgagtggc
     8221 acgagtctca tttctgtggg catcgtggca atctttctat gttgtttcgg agatgcaaag
     8281 agtttcgcca cctcccccga ggcgccagag ccaccgccca cgttactatc gcagaacggc
     8341 tcggcagtct cccatcccgc agaagatcgc ggtccatcgc gcggaagcac gttgggggcc
     8401 ctctcgctga tcgcacaaac gacgcagctt cctggccgcg agtcgtcggc cacaactccc
     8461 cctggtgtca ctgcccccgg ttcagctacg cttccgcctc cttatcgcgt ttttattccg
     8521 gttgatgaaa cgggaaaacc ggtaggacag gaagtctatg tccccgaagg gctttacaaa
     8581 gagcttcaga aaaggacttc tcccaccacg gcaaagccca gtggatggca tttcggctcc
     8641 gcgacctacc gtggaacgct tgcggccacc ccggcgggag accgctggga actttcgcaa
     8701 ctgacggcca actttgaagt gtgggttgtg gatgccccaa cgcagctcca aatacccctg
     8761 ggaagtgcgg aagccgtccc tcaactgaca gatatcttgc tcgatgggcg gccgattgtg
     8821 gccacctggg atcaatcgcg gctccaggtg aacctcccag aaagtggacg gtaccgaatc
     8881 caggtcccca tcgcgctaac accgcgaatc gatgcaggga ccagcgaggt ccgcttgcgg
     8941 attccgtcgg ttgcaaacac gcggcttgaa ctggcggctc ccttcgccgt caatcgtatc
     9001 gaagttccct ccgctcgggg acgtgtggaa agcgacccgg aaagcgggcg atggaccgcc
     9061 gagttgggcg caatctcgga acttgtcatt cgctggccgg ccagtagtga tcccctctcc
     9121 gacacaggtc cccaggccga catcctgtac aggttgcgat tgggcccgtc ccaggcaacc
     9181 tgggaattgc gatggaagtt ccatttgaat aacgcccaaa tcgatcaact ccaggtattg
     9241 tgtgaaccaa gtttgcgtct cgatcaggcc tctgctgata gccaagtgcg tattgaaact
     9301 ctcgtcgctg acagtgatcg ccaaatcctc caaatgggat ttgattcacc tctcaaggat
     9361 gagggcacct taatggccgt atttacggaa ccgcttccca caggctcagg tggcttgcga
     9421 cttcccgatg tccaattgat gggaattagg attgcgagga gaaccctctt catccaggcc
     9481 catcctggtg tttctcttga tgtacgtgag tcatcgggct ggcgcttggc cgatcttgtt
     9541 accgaaagca aacgctgggg aatacctcca ggcgaggtgg gcgaagtcta cgaatacctg
     9601 ggcggtccca tgttttgggg tgcttgggta cagcctcagc ccgtacggcc aaccatggat
     9661 gaacacgtgc aacttctcgc tacccaagac gagatcctca tgcgatggca agctcggttt
     9721 acctcctccg gcggaatatc cggctgtcac cgtgtccgca tccccgaggg tttcagtgtc
     9781 cgtaaggtgg ccgtgattac cggcgctacg gagcaacctt atccctggtc cgtttccgac
     9841 gatcaaacgc tccttcttct cacggatcgc cttggtcccg acagtcaatt actcattgaa
     9901 ggacgtttgt ccctgccact gagtgaggac tggcaccttc agcggcccac attggctgac
     9961 gctgaagtgc aaaacctaac cgtgatgctt ttccgcgcac ccaacgtctt cgttgaagta
    10021 accaagcacg tcggcctgga actggtctcc tctgttcaac aacctaccgg cgaccagcaa
    10081 ctcggcgtgt tcctcaacgc ctatgccgca cgacaaccgc aagcagtgga cctgaccttg
    10141 cgcattcagg ccaatcaacc ccaaattaca gcggagcagt tcatacgggt aacggaagcg
    10201 ggaaatcgac tccaggcaga gattcgactc gacgtgacga tcattaacgg gttcttgcaa
    10261 gagcttaaac tcgatttgcc gccgcagtgg caaattgggc cggctggggg tgaaaggacc
    10321 gcatctccac tggaattgaa aacggagtgg ggcctgacca acgctggtcc cgcgggctat
    10381 gagacaggcc gcgtgaagaa aagactgacc tttgcggccc ctcaaaaaga cacagcgtcc
    10441 gtcgcgctct taggcagccc cacgcttgac gaccacggtt gtgtgcccat cccttggatt
    10501 ggtctctcag aagtttcctc cctccgatcg taccttattg tgccgaaacg tctggagtgg
    10561 ccaagcgatc ttgcgaacat ctacgggttg cgacccgtcc ctcaagaaga gttaaaagga
    10621 atcaacatca aggcagacga gaccgcatac gaattgaccc aaacaatgat ccttccgcac
    10681 atcgtaggca aaccctcccc tgatgctcgt tgggaagatg ccgaaatacg aattcaagtc
    10741 tctcccgagg gacacatctt gggcctgctt actgtgtggt tcacaagttc gcctgcggag
    10801 ggctatcaaa tccaaatgcc aaaggggatc gttccggtta atgcttggct cgaccaccgg
    10861 tgcgtgcccg tttaccagcg ccccgatggc acaacccaca ttgccacaga tacggtcaaa
    10921 gatcaccggt tggacctcct gttcgccggc agcgtcgctc ggagaggtca gagccttagc
    10981 ctggcacttc cgcaactgaa gaatgcggaa aaagcagaat ccgggcgggt ctatcttctg
    11041 ttgcagggcg cctccagttg gaatccgtcc ctcgcccctg gtgagccggt cactgcgtgg
    11101 gactatcact tgacccgcgc gcgagaggcc gccgagcgtt tgctccaact ctcagaagga
    11161 gcggcaggtc ctgcgccacc tcagggtacc tttggggaac tgctaaagca gtacctcttg
    11221 gcacgagacg aagcacgacg tgcccttctg ctggcacctc aaaccgctgc tatcagttac
    11281 gcggaagtaa ggcttcaaaa tcttgaattg caagtgagcg atttgttgca atctgccgtg
    11341 gggattgaag ccacactggc gtcccctggg gccatcgcag gcgaaaccct gggtgacgcg
    11401 ctaccatcgt cggatatgca atcctggtac tttgagtgtg aggaccttcc cacacaaatc
    11461 cgccttcaac aaaccgttag cgcccaaaaa tggggagatt tgcttatcgg cagcgttgtg
    11521 atcttcgcgg taggtagcat aggctggctg atccgtcgca tcaatcggcc actttccgta
    11581 tggatgatgc gatttgccca tcccttggcc gttctcttcg ggatcggttg gtggttgtgg
    11641 cttcgtccga gctttgtcgg ctggcttctc atccttgctg cccttattct gcttatccga
    11701 tctcgctggc aatgggtgcc taaagaagat gcaatcgtgc ccttaattgt gcaacagaag
    11761 ttgccaacaa cgggcagtcc gcgataattc cacgtggtgt gtgacacgtt gcctccctta
    11821 cttccacttc ttcgcttgcc aactggccgt gtcatgcttt tcggcacggc acttgctgtg
    11881 ggacttgttt atagcctatc ccaaagctcc ctctctcccc ctgggagagg gttggggtga
    11941 gggcagacga ttttccgagt cgatcgagaa agccgacagt gatttgagcc ccctcacccc
    12001 cggcccctct cccgctgaac gggcgagggg aggttgtggg ataggctctt agtccacagt
    12061 ggctgtccca tggatgggtc catttagtga aagcggcttg acctagttgt gtgccgagtc
    12121 cgacttggcg atggatgagg tgttatgtcg cgtaaagtct atatcttcca aaacgcccgt
    12181 ggcgaacccc ccgggcagat cgtcgattat ctcgactggg tcggcgtacg tttcgaactg
    12241 atcccactgt tccatatccg cgagagtgtg tcccctctgg attggcgtga ggtatgcggg
    12301 ctggttgtcc tcggcggccc aatgaacgtg gatgaggtag aggcataccc ttttctgatg
    12361 tgggaacgac ttcaactgaa agaggcagta cgccgcgaga ttcccgtcct cggaatctgc
    12421 ttgggggccc aattgcttgc ccgcgcactt ggggccaaag tctatcccaa tccggttcgc
    12481 gaaatcggct ggtacagagt aacgttgacg gaagggtgcc aaagtgaccc gctgtttgcg
    12541 ggcgcctcct caccggccac cgtctttcaa tggcacggcg atacgtttca ccttccagaa
    12601 ggtgctgtgc tcctggcaac gggatcacag tgtcgccacc aggccttccg ttatggtaac
    12661 aatgcctggg gcctgcaatt tcatgtcgag gttacgccta tgcttatccg atcttggatg
    12721 gaattggcca cgcaaaacaa cgagttggcg gagatcggac tctcggccag ccaaatcgag
    12781 caggaaacac cccatcgctt tggcgaaatg cagcgactgg ccagaatcgt cctgaaacgt
    12841 ttcgccgaac aatgccgcca atgttccgat tagccgtgtc tcacacagac tgatggtgac
    12901 tcagagagcg gctaagcctc gcggtccacc cgagggttcg cacgctggat cacgacgatc
    12961 gataccacgg ccacaatacg caaataaccg gcatccgcca tcttcaatag tggtcccatc
    13021 tctccgtctc catccagtca tttccatatg gtaaactgtg gcggacgtgt tggtcatctt
    13081 tcatcgctaa gagcctatcc caaagttccc tcgcccccct atgagagggt tggggtgagg
    13141 gcaaacgatt ttccgagtcg atcgagaaaa ccgacagtga tttgagcccc ctcacccccg
    13201 gcccctctcc tgccgaacgg gcgaggcgag gtttgtggga taggcgctaa gccttaattt
    13261 caacggaagg cccaacctat gctgacttcg cgggcactca ttgaaatggc cctggccggt
    13321 caaccgacac cgcgacccgc aaccggacca cttgcggtgc acttctgtgc caagttaagt
    13381 gggatttcgc tgcgtcagta cacgctcgat cctaagcaac tcgcccgcag tgtgatcgaa
    13441 tattatcgcc gctttcgccc agatgcagtc tggttgtccg ccgacacgtg ggtcaacgct
    13501 gaagcgatgg gcgcccgtgt cggttttccc gacgatgatc aaccggccgc cgggttgggc
    13561 cccgccctga ttcaatcgcc tgaggacctc gacgccatcc cgccgcccga cgttacccgc
    13621 cacggccgat ggcccgccat gctggaggcc gtaaggctcg tccgccaaca attgggcgac
    13681 gacgtatgtc ttgtggcatg ttttgatcaa taccccttct ctttggcgag tcaactattg
    13741 gggctcaatc gcatgatgct tgccattttt gacgaccggc cctttgtgga gcgtgtcatg
    13801 gaaaaatgtc ttgaatacac gctggcatat gccgtggccc tcgccgaggc cggggcggat
    13861 attctaagtg gtggtgattc gcccgcggga ttgatcggtc cccgctcgta tcgagaagta
    13921 gcacaacctt tggaaaaaag gctcattgag cggcttaaag agcgtgtccg tgtgccagtg
    13981 tcgttacaca tctgcggcaa cgcaacgccg ctgcttgctg acatggccgc cacaggtgcc
    14041 gatatcctgg aaattgacta tcccgttgcc atcgaagacg cggcacagat cctgggatca
    14101 tcagtcgccc tttggggcaa ccttgaccct gtgggtttgc tggcccaggc tgatgccgat
    14161 accgtctatc gagaaacgca agcacttctg cggcgtgttc ggcaatgcgg cattcggcgg
    14221 ttcgtcgtca gttcgggctg caccctggcg atggagacac ctgaagagaa tcttgatgcg
    14281 tttttcgccg ccgcaagaga ttttggacct tggggagata agaacgggtg aacgactgct
    14341 gcccgccttt tgcttagtat ggtcatcgct tttaaagatc gtgaatacga agagtaagag
    14401 tgggagacgc tcgtatggct acctcagtat ttggtgtgct tgtccatggt gcgggatggg
    14461 tttcgagtca gcacatcgcg gcctaccaga agaatcccgc cacagagatt cgtgccattt
    14521 gcgaccgctc tctcgagcgc gcccaagcaa gggccaagag tgcaggccta accaacgtgg
    14581 caatctatga cgatttcgag aaagccctcc ggcatccggg gatcgacatc gtttcgatct
    14641 gcacgcctca acacgtccat tgcgagaacg ttttggctgc cgctgccgcc ggcaagcata
    14701 tggtgatcga aaagccggtg gccatcaatt tgacgcagct ccgcatgatg caacaggccg
    14761 tcaagaaggc aggcgtgcgg acagtggtaa gtttcgtcct ccgctggaac ccgttattcc
    14821 gcacgctgaa ggccctcttg gccgatgggg ccttgggcca accttactac gtggagacag
    14881 attatctcag ttacaacggc agttggtggt ccgggtggaa tgatgcaaga accatcgagc
    14941 agggagtgag cgccttgctg gtgggcggat gccacgccgt ggacgccttg cggtggttcg
    15001 cctccacgga gcagttcaaa gcggccgatg ccgaagaggt ctttgccgtc tctggtggac
    15061 gccgaaaagg tcaaacccgc gagtacaacc ccctcacaaa cacctggacg gacaacgccc
    15121 cgcccatgga atatgacggt ttggaagtag ttctagtcaa attcaccaac ggcgtcgtgg
    15181 gaaaggtctc ggcaaatgcg gagtgtatta tgccctaccg gtttcctatt agaatctttg
    15241 gtgatcgcgg taccgcattc gataaccgcg tgtggtcgca caaattcccg gaccagacag
    15301 attgggtcac catcccgacg attgccccgg attcaagtga cgtaacccat caccccttcc
    15361 aggccgagat cgatcacttc gtcgagtgcc tacaaaaggg gaccgaatcg cattgcaacc
    15421 ttgacgacgc ggcaaagacg cacgagatcg tgtttgcggc actggaatgc taccgccagc
    15481 ggcgccccat tcgcttgccc ctggcggaat gagcgctccg ccttttgcga gaacgcatat
    15541 cgtttggcga actcgacgct tggatactca gtagcttcca ctaggcagag catgtcgctg
    15601 tggctttcgg ggaatcgtgg ctcggggtgc ccttgcggat tattgcctcc ctcagaaact
    15661 ggctttgtga ggaacaacct cgcgcatcac gatcggaccc gcgtttggct ttggctaggc
    15721 ctaggctctt ccggtggagg cactttggga ttttgcgcca gacattgact taccgacggc
    15781 gtaacctgtt taacgacccg ttcagcgggc taatagagac aagcacgccg cgggcggctg
    15841 ttgaccaggc tttatcggac acgaagcaat caggggatta agcgaaggcc caagaccgac
    15901 caccagcgcg tcaagagcct atcccgttat ctctcctcac ccgtttggcg ggagaggggc
    15961 cgggggtgag ggtggctcga atcgcgctgc cggttttctc gttcgactcc gacgatcgct
    16021 tgccctcacc ccaaccctct cccagaggga gagggggact tttggggata gactgttaac
    16081 ggtttgggaa gcctggaatg ctttgattgg gtgagccaaa cggagaaaca ccacaggatg
    16141 ccgcagataa gtcttcagcg ctggggttgg atcgaagatt gctctcctcg gaagcactgg
    16201 gccgtgcaaa cgtggcgaga tacgcggtcg cggtgtgtgt cgataaggtc ctgagggcag
    16261 tgaaagatgg attcctgttg gtcttggcca actccacagg tcaaaaaggg ggccccgggg
    16321 ttgggcaaag gtcgtagcgg gacaaccggt gaaagcccga gccacacacc gggccacggc
    16381 aggcacgggg gccagcccct acggtttcga tgggccacgt gtaggggcaa ccccccgtgg
    16441 ttgccccttt ttgcgcagcg cccacggttc gtgtttgggc gggcacccgt tgtttggatt
    16501 tgatggggac ccaggcgatg tttacggtgt agggcaggca cgggggcctg cccctacggt
    16561 tttggtgcgg tgcgtgtagg ggcaaccccc tgtggttgcc ctgattgtgc cccccgtggt
    16621 tgccgagact acgataattc cccggggtgg ccggtgatct ggcgttagtc ttgtgttgaa
    16681 gcaccccgtc ggcagttaac cgcggctgtc cgccagatat ttccgcccaa agtccgagat
    16741 ggcccagatt ccccgcgatg aatcggcttt gagaaaccct tcctcggcca tactatggcg
    16801 cgcccattgg gcggcgctca attattttgg tctggcaggt tcacagcaca taccggaacc
    16861 tgctgcggac tcaagatttc gttcgcaggt catttttttg cgcggatgat aagagcgggg
    16921 ccaggccaaa ggatttcgag catgtgatgg tcagtctcgg agaaaatctc gaagatgttg
    16981 tcaccaggcc gcaacacctg aggaggaata gagtggatat gatagtgata atggtggtta
    17041 tttccttcgt gcggtcgctc atagtcgtta agtttgtaag gtgcatgatg cttatcccaa
    17101 ccgtgccacg tgcggtaatg tagcccagcc tccacgacgg cttcggggtt ataactttcg
    17161 ggcagcctaa tcgaacatga acggcgctgg ccgacacgaa caccaaactc cggtggaaaa
    17221 tcgcgcacac aatgcagggt gaccgtttca ttggggcgat gcaatgtcaa ccccgtcacc
    17281 tcttccgtga cagaccaagt cccagacttg tcgcgaatgc gcgcgatcaa acgaatctgt
    17341 ccgggaggct ggtcgggaac ccaccgggta ttccaagtga cttgcaacgg cgccttgagc
    17401 accgttccga cgtggtcacg ccattcggcg ggctcgttac gattcggctg ataccaaccc
    17461 ccgtgccaat caagaaagcg gccatctccg ttttcgtcgt agccctcata ccaggcaaac
    17521 acttcgatcc ggtctaattg atcttcctta tcgacagcta ttgtgattac cgggttttcc
    17581 tcgatggttt gctgggaaag cggagaaaca atccggcctt ttaaatgtgg tttctttgtt
    17641 ggatcgaaat acacccgcaa cataagggaa tacaggcccc attgtcccca tcccccaggc
    17701 ttcttgtgtc cgcaaattcc ctcaatgatg tttttgcctt ctttaagatg ttccagcggc
    17761 accgcaatca caggattgtc ttggaaataa taattctccg gaagcgagac gccatttccc
    17821 atcgaaggag gagctaggct cagccattcc tgtttgttaa agcgaattgc gggattggtg
    17881 gtatccagat gtccacccca acggtcgagc atcacctcgg cgcggattgc tccttcaagg
    17941 tcgtctattt ccaactccca gattggattg ggtaagaatt tttgggctcc ggaatcgcgt
    18001 gcctcaggac tggtcacccg ccaggcacga tcgcccgagt tgtgaaggat atattcctta
    18061 tagacatccc cggcgcgagg acctaactcg gccgactgag tccctgccgc ataaccccac
    18121 gtcaggcatt ggacaaaaac gacaattaaa acaccatctc gtgtgaccac ggtcttcctc
    18181 catagagtgc ttaattttca cattctgtca cttgttatcg tgcaccagcg cgaacttgcc
    18241 cctgtaacac tcactcagcg ttagatgtct tgcgaatggc tttattcaca aaaagccaca
    18301 ccttgggatt gtgccaacct atggatacga tatccaggtc accatcacga tcaatgtcgt
    18361 atgccaaagt tccatcgtga tggtcgattg tgcccgaact tccctcgtca atcacaaccg
    18421 ccggccaatc actccagttc ctcggttgaa ggcgtccggg attccgaaaa acaatcaccc
    18481 ggttcacctt gaaaccccga tgttccccga ctaccacatc ggggtagccg tccccatcaa
    18541 aatcggccac gtccatactg aatccttggc catcaaccac gccgactcga tgcttatgcc
    18601 atacactcat gggatcgact ggttgttcaa accataacac gtcggtgcct ttctccaagc
    18661 taacaacagc atccaggcgc ccgtcacgat tgacatcagc caggcggcag cggtctggct
    18721 cgccctgttc aatatgtccg agcacgaccg tggtccactg gccgttgtca tttcttagcc
    18781 acttgtcacc caacaaaaga tctaggtcac cgtccctgtc aatgtccccc aaagccaaat
    18841 cttcaccttg aggtggtgtg cttaccgtct gacttatcaa ctgtgagctc catggggaat
    18901 tcgcgggagc ttcaggtacc gaaaggatgt ggatgccgcc gccgtctcga tgccaggaga
    18961 gaaatacact caaaccattt ggaaggaagc cgacgacgca gccttgcaga aaatctccac
    19021 cgcccgagta gtgtacgttg tgcagtatct cgaactctcc ccgccccgta ttgcggaccc
    19081 acacgaattc ccgattagcc tcgctccctt cgccttttgt ccccagaacg tccggaagcg
    19141 tgtcaccgtc aaagtcgtaa accgtggcca tgttccgaag cggcaaacca atggaatgtt
    19201 gtcgccatgg ggtaccaacc gcaccaggat tttcccacca ccatgcccca gccacaatat
    19261 ccagcagacc atcgccattg atgtccgcgg cgttcacaaa gacggcacga tgccgcagtt
    19321 gcccgacctc gactcgtgtc caacggtcca gaggtaaatc ctgggtcccc atattcaaaa
    19381 ggacgtcgat gcggggagcg cgatggctat agggttttac caggatgtcc ggacgcccat
    19441 cgccgttaaa gtcgcccgct cttccctcat gaatggccaa accatgccac gcgattgtct
    19501 tgcgaaagct tccctcaccg tcaccatacc aaatgaaaat ctttgcatcg tcaccggcct
    19561 caggcgccac gaaccgccgt cggtatcact acgacaccga gcggttctac cgggaattgg
    19621 gataaaggtt gggcgggttc ggcacgcaat cgtccgggga caatagtaat ggtcaatcta
    19681 ttcagtcggt gtcgtagcca gccaggatcc gtcttcactt tttggttttg agttctgctg
    19741 ggaagaagaa cgagaagcca cacctcaagc gtcacacatc acgcaagaaa cactgcgagc
    19801 tgctgaagcg cttgaccgaa tggtgcaagc ttagtcaccg ctacaggtta aaggacttgt
    19861 ttagggactt gaacgccaag gtactcgttg ctacaagtac tatggggagt ggcagattgt
    19921 ccccgcgtga gccgcttttt ccgaatggtg atgcgcgctc aggcgggacg gccacacacc
    19981 ttagggcgac ccaagacgaa gaaggggctt tggaggctta gggcccgttg cggaatcgag
    20041 aatttctgaa aaacccgatg tggtaaactg catgctgcca tctaggcggt agtagttgga
    20101 taaacggcct tcccagcgcg acgcatggtc gtaaaggtgt attgcctaga cgatctcgtt
    20161 catcaaacgc aatatgacag gggtcattag agtgttcatg aatgcaagaa aattaagaaa
    20221 actcgtagtg gacagactta aggaagcggg ttttttgata tcagctaatg gcacgatcca
    20281 tcttccgttg gttgacaagg aaagcctgcg tcgaatgcac aaaccggcta cgcaattgga
    20341 aattgcgcgc cgtcgggatt ggcttcttcg acatctctcc gagtatcttt gtttctttgc
    20401 cgatggtgac gaaatcgtac ctgaacgcat tactcccgcc ttggttgaag ttaaaacaga
    20461 tgagcaacac aatctctttc gtgtagcacg gttactttgg tctatcccat tctctaaggg
    20521 ctacggtcgg cggttgcgat tcttagttgt tgatcaggct aacggaaaac tgattggcct
    20581 gctggctttg cagtccccgc cgctcagttt tccggcgagg gatcgcctgt tttgttaccc
    20641 acctggcaag aagacagaac tggtcaatca gacgatggac attcaaactt taggtgcggt
    20701 accaccctat gcgcgtttat tgggagggaa gcttgttgcg ctagcagcag cctcgaatga
    20761 ggttcgccaa acctatcgac ggaaatactc cgggcgtgcc acggagatgg agaagcgtac
    20821 gctgccacct cacctcgtgg ctcttaccac aaccagtgct ttcgcccgaa gcagccttta
    20881 caaccgccta acctacaagg gaatgcgtat cgccgagtcg ctcggttaca cggaaggcta
    20941 tggcactttt catttgatgg aactttacca gctgttccgc gagtttttgg agggtcaagg
    21001 aatttccacg cgaggtggct tcggtaccgg gcctcgtcaa aagtggcaaa ctatggtacg
    21061 agcgttggaa cgattgggct tctcgtcgga gttgcttcgc cacggtataa aacgtgaagt
    21121 gtttctattt cgcttaattg acaacctaga gaattacatg gaagggcgtg atactgaacc
    21181 cgtttatcgt gacctcccat tctccgattt ggttgcctgg tggcgagaac gctggttgct
    21241 gccgcgagca gtgcgtgtgg atgggtggcg agagtggcgc aaagaggaga tcaagagtct
    21301 attgctgatc gaacaggaga aggccacaaa atgatggaac ctgtagaacg tccctttggc
    21361 gacctccctg ctgctttagt ggacgaggtc ctcaaacgcg gagaggatct tagccagaag
    21421 ttgctaagtg atttcgagca ggtgcgcact cgtcgccagg aatggcgaga aagcctgaaa
    21481 ggggcacgct tgcttcgccg tgacgctgaa cttcgatatg tgcctattcc gacgacgtgc
    21541 ggtgtagatg gctcctatgc ggtggagcga ctgctggcta gcgacttggt cgccgcggct
    21601 gccgttgccg tagaaggctt aacaccgcct tcagaaacgc gacattggcc agaaccccgt
    21661 catcgcgttt gggtagagat agaggctcat gaaaccgaaa caggcacgat tttacgggcc
    21721 ttgatgatcg gcctagagct ggaactagcc tctcaggccc cgcatgaggt tgtattcctg
    21781 gatgggtctc ttacaactgc tcttatttac ttcaaccagg cgttgaacaa aagtcgagat
    21841 acttcacatt tgcgtactag aaaccacttg cttgagcaaa caccctcatt tctggaagcg
    21901 tactcgacga tacttacctc aactcgaagt gaccgttgtt gggtggcggt acccaagtac
    21961 acaacgaagc gggaaattgg gcaggagatg ggatggcctg aaacacacga cgaccgtggg
    22021 ctcttaagct ttctgctcga accaggtgaa ttcacaagtc cccggtcact acagcagccg
    22081 tccgagcctt ggcacttgaa tctggatccg cttgactgtg cttctaggaa agcaaccgac
    22141 cacatcgtag agaagatcat cgcgtcattg ggcgagatcc aggtcgtcta ctatcgtccc
    22201 tttccttggc ttccagccct gcgagtagaa atgagccgtg cagtggcaga aaatccagcg
    22261 cgcttggcta cggtactgca tgcgattcgc caccaatgtg gtacgtcggc cgtgctagaa
    22321 ccctatcctc tctatatggc ggatcggatg gtaaagcact tgccgaaggc gattcctaca
    22381 tttcggcaat taaccagtca atatatcgcc gagacttatc acggagacat cggggaggtt
    22441 tttctaggcc tccatggcta tcgcactgag tcaggagtat gatccgatgt cgcagaatct
    22501 tgatcaactt gtagataagg ccgaacggat tggcgtcgta gggtccccat cctctaccag
    22561 tgaaatggcg ctggacatct tggcgggagc cgtcaacaag aaactggtag gggaactggc
    22621 gttgtttcgc tatcaccagg acgggctttc tcattatgcg ttggggcaga ttaccgaggt
    22681 taaactccgc aacgtctggc atgaggaccc aacaatgcgc agcctgatcc gtcagcgcgg
    22741 gcggttggat acagttagcg aacgtcagga cacccatttg ggagagatga tcatcagcgc
    22801 tgttttctcg gccggatcca atggctatcg ccccagcatc ctgggcactg tccccgccac
    22861 gggtacacct gttcatcttg tcaataatga tgttctgtcc gaactcctct cagactacca
    22921 taaacaactt ttctacttgg gacacgtcta cggttcaaag cccgtgcggc tgccgctttg
    22981 gttcaagcat ttcgataccg gtcccgaagg tgctggagag gcctaccatc tgggcatttt
    23041 tggcaaaacc ggttctggta agtctgtgct cgccaagatg attctgctgg cttatgcccg
    23101 ctatggcggg atggcaattc tcgtcattga tcctcagggt gaatttgcca aggaaatacg
    23161 gggtgaagca gtaccgggcg gatttcagtt gccaatgaag aaggttctag aaaagctcgg
    23221 gaaaccagtc cgagtgcatg gggtgaagga cttggtacta gatcggtggg atctgttcgc
    23281 tcaaatcctc tatgagtcac ccttttttga acagttgagc attcccaagg gagagaatcg
    23341 acagttggca gcagagacac tgaccgatcg cctgcaacgc gctaaagtta cgctgaagga
    23401 tttacatgcc cgcgaatcgt tcgatcgcgc atggcaactg ctcggtgacg aaaatgtgca
    23461 aaaggtcttt taccgtacag aagggtcgcg agctcggttt cggtctatgt atgaccaagc
    23521 cgatcctcat ttcttcttca acacctactg gcttccggtt acacaactgt ttcgtgaaga
    23581 tcgaccaggg gcacagaagg ttgagggatt attggctagc ttgtttaacc ccgtccaaac
    23641 ccagcgtccg ctcgttgtga tcgatttgtc ggttgagcag gcgacacagg ccagtagcaa
    23701 agtagaaaac cttccccttt ttggcggaga tcagtcgcag tcccagggga atgccctatt
    23761 ttggaacgag acgatacaat cactggtcat taaacggtta ttggaaggtg tgcgtatagc
    23821 ggcagaatat gcttacaagc agaaccgcag tctcaatacg ctagttctaa tggatgaagc
    23881 acatcgcttg gcaccccggg aagatccgga gagcgacgag aaaagagcgg tgcgtgggat
    23941 tttgattgac gcagcacgca ctactcgtaa atatggagtc ggatggatgt tcgtcagcca
    24001 gacgctctcc agtctgcatc gcgaaatctt ggagcaatta cgcatctttt tcttcggttt
    24061 tggtcttgga atgggagccg agttcaaagc attgagcgaa ctggtaggag ggcgaagcaa
    24121 agcccttgac ctctatcagt tgtttcgtga cccacattcg tcgttcgaca tcgcaagtag
    24181 agaatattca ttcatgacta ttggcccggt ctcgccactc tcgtttgccg ggacaccctt
    24241 gttcttcaac gctttcaata gcgtggcgga gttcctgaaa gccaattcac ttaacgaata
    24301 agcccagctt agttatgctc ggcgcggtgg ttcccagcgt cctcccgccc accaaccaga
    24361 cgtggcagtt gccaaccatc tggctcctca gaaagagggg cccagtcatt gtactagcca
    24421 ccgggtgcag catcgtgacg gtcaacaggc aacaggataa ggcaaagtgg gcgttggtgg
    24481 tgtgattgcc gtgctgttgc cgcacttaca gcatggccgc caccgtaaaa gggtagttca
    24541 tggcaattgg aacgaccttc aataaccagt tcacgggcct aagcgtggtc gcgtgacgcc
    24601 ttgactcagc gtgccagtgc accagatcac ttctgaaggg atccttgtac cagcgcactc
    24661 tgagatgtcg gatgattttt acttcgcgat cccgtaccag ccaactgatc ccgttcgaga
    24721 agcttatgtg catgcctacc ggccaccaat agcattaggc aagcatgcgt tctgggtatg
    24781 gaatcgcaac cgtgccctgg ggtgtaccgg ctgatcgtaa tgagacaaca cgtgctaaaa
    24841 tccacgccgc gagacttcct tcttttctgc agccagtcat cgtggtccta ggtggcagta
    24901 ttacgtgagg agcaaaaaga gattcggctc aacgccgcga cggtccgtcg cgggctccat
    24961 catatgcagc ttgtctgagg tccaccatgc tcagtggtcg attcccggca tgtggcatga
    25021 cagccggctg acttgtcgcg tctggacaac ccccgccagg gtctcccccc gtttcccaag
    25081 attcacgtag tctagaacta cgctttgtct cataatggca gggcaaggca ggaatacgtc
    25141 gccgattggg ggcgtcggat caaatgcgct ttttgcccaa atctgcccca aaagcaatca
    25201 cacggagcgg gtacgttagc acctgcagga ggtgattatg tgtaatcatc gtcattgcac
    25261 gctaacaaac tgcgaaaagc aacacgtcgg cgggcagcca cccagcgcac gtcttaccaa
    25321 caaacttagg gctttcgcac catcttcacg cgcaatgagc gtgatgcttg cgagatgaag
    25381 tcatttaaac cctcacaaag ctccccttgc ggtacataaa gaacaggagg agccacagaa
    25441 ttaaaaacgc ccccaccggg ttaagggctt cgcccacgtt ggccagcggc gccccgaacg
    25501 tcgccaggta tcgggccagg ccgcccacaa atcgctcggc gatcgtggga aaactgacca
    25561 gattccacgc cacgtacgct gtgatggcat tggccccgag gatctcgaaa aaccaaccca
    25621 cccagcgatg gcccagcacg tcgatcacca aatagaacag cgccagcagc aagtagctcc
    25681 agccggcggc ccacagcacc atcgagctgg tgaaaaggtg cttgatgata ggaaaccgcc
    25741 agttgcccac gagggtcatt tggaaccact ggtcaaaacc acccgaccac agccatgcca
    25801 cggcgaggca tccgacaccc cacagcacaa gccacacaac cttcattcct ctagaccagc
    25861 gtgagcggag gagatgccca gcaaagacgc ccatcaggac catcccggca aaggccatac
    25921 tgcttaatat ccatgtataa gttgtaccgt cgcggaaggg gccaagcacc tgttcgtcga
    25981 tatacagggc taggttgacc ttttcttcca gcgtaccagc cgggtgacct cggaagggca
    26041 ccagcagcat cagcagccaa tagccaacta gcagcaccgc agtcccggcc acttggcccc
    26101 agaccggcaa gtgaaaaagg aacaccgagg ccaccaggta gcccgcggca atggcttgaa
    26161 gggtgttcga gtagaaatgg atggtctggc ggtcaaactc cagcagatgc ccctgcacca
    26221 tcatgcccag aatccacagc acgagcaccc ggcgaagcac tcggaggtaa agtgatttct
    26281 tcgtctggcc ctactcgatc cgccgagcaa acgccaaggg catgaccagg cccaccacaa
    26341 acaaaaaaag cggcatgatg aggtcccatg ccgtgaagcc ctcccagcgg gcatgctcga
    26401 tctgggcttt gaaccaatcg ggcaggggtg agacgaaaat agccaggatg gccaaaaagg
    26461 cctctcgtcc tccggcaatc caaaacatat cgaagccccg gagggcatcg atcgcggcga
    26521 ttcgccccgt ggtcggtgcg gcaggcgttg agctatgcat ggcgcggtct cctcgtacac
    26581 ggctccaggg aacatgtgaa gcaacccaag gccccggcgc ccgccaacgg cttgccggaa
    26641 acatgcgcgg catcggctcg ccagtccagc ggggttcacg ctcgcgatta tgattggcca
    26701 attagatcaa gacaagctgg ggccgcccag ggtatttcga cagggcatag gggttaagac
    26761 aggcccaggg cctagggcag gcacgggg
//
LOCUS       DAOSUT010000044        26737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1194_length_26737_cov_9.502698, whole genome shotgun sequence.
ACCESSION   DAOSUT010000044 DAOSUT010000000
VERSION     DAOSUT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 26737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26737
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_26737_cov_9.502698"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLD05_05710"
     CDS             complement(<1..902)
                     /locus_tag="PLD05_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76966.1"
                     /translation="MRKWVNDRIWWRFGPAAGMLLVVILAADGCVAPATNTAPGAPVL
                     PPADESGGYYREVPGPILAGDCSEFMVTPQETAAAVGSEVILVASVRGQDQFMLTNQR
                     VEWTITPGGVGHFVDFRRGTFMDYLVGDFTRPRKITEHYVITSTSREYLRLTRGTVAP
                     NDDVIVRPGQAWVSVTSPVEGTTAINVYVPCVHSWDRRVQTVFIHWVDAQWTLPPPAI
                     VPAGSRHQLATTVTRLRDRSPCAGWKVRYEIIGGVSALFAPNGTTSVEVPVDSTGRAT
                     AEIYQAQPLGGSTQVAVQIIRPADA"
     gene            complement(1897..2787)
                     /gene="xerC"
                     /locus_tag="PLD05_05715"
     CDS             complement(1897..2787)
                     /gene="xerC"
                     /locus_tag="PLD05_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOP76967.1"
                     /translation="MARFLRFLAVEKNASPLTIKSYREDLTALLEYLTEAYQGRCPSP
                     SQITTLDLRGYVAAMHEANYSFATVARRLASLRSFFRFGMREGWVKDNPAKPLRNPRK
                     PRNLPHVLSTEEVRRLLEAPPANDPMGLRDRAILETLYSAGLRISELVGLNDSDLDLR
                     AEVVRVRGKGRRERLAPLGSHAVRAIRRWLAVREIHPSFVDNPDRPLFVNRFKLRLTT
                     RSVARMLEKYLIQTGLDQRTTPHSLRHSFATHLLNAGADIRSVQELLGHKSLVTTQIY
                     THLTTANLKAVYEKAHPRAR"
     gene            complement(2883..3128)
                     /locus_tag="PLD05_05720"
     CDS             complement(2883..3128)
                     /locus_tag="PLD05_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76968.1"
                     /translation="MSREINVIALIKGQERYIFLYDDDNRLEMIRTLGRYAANPELSF
                     SWYDAAVLSQKVRQEARRRALQNRFKAPQPADPEDIF"
     gene            complement(3213..4238)
                     /locus_tag="PLD05_05725"
     CDS             complement(3213..4238)
                     /locus_tag="PLD05_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76969.1"
                     /translation="MANVLYQQQRTADIPRGFTRLGGSHSAPWMLDRRLSVALILTVL
                     VFPAGCSLFQKPEWRPLVHNPFPQIHKIAVAPFFNLSTDPTLDGRKVAIAYYNELQEV
                     PGFEVIPVGVVERAMFQHRISLNNPQDVRHLAQLLGADAVVIGAVTEFSPYYPPRCGL
                     QVEWYAANPGFCPVPPGYGLPWGTKEEKDIPSRLVLESQLAYAREALRNQSPQAADAS
                     ATDAAAQPGFEIPQTGEGKSQASLSPEGLIPKLAQAGDSACGAGEEPFMKHTRVYNGG
                     DSQLVKLLQDYAKFRDDARLGGWQSYLTKSEEFLRFCCRVHIWEMLTARGGADESELV
                     WSTTSGR"
     gene            4580..7336
                     /gene="fliD"
                     /locus_tag="PLD05_05730"
     CDS             4580..7336
                     /gene="fliD"
                     /locus_tag="PLD05_05730"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOP76970.1"
                     /translation="MGRIQTNVGLITGVPIGETVNQLMLIESRPKENLESANKKIDAE
                     RTAITELSVLFLAAQYPIKNLLKDAVYTKRSATSSNPSALNVRVTGTPSVGTYVFTPI
                     RTAQSEQWLSSGVRESTTPLGGGVLSFRYGPSIDRPLALDAIRGGQGFERGVIRITDR
                     SGASAEIDLTTVQTLDDVIEAINRNMRINVLAEVVGDHIRLTDRTGMTASNLRVQDLG
                     NGRAAASLGLAGIDVAESTADGSDIVWLTTDTLLSDLNDGLGISASSTLPDISFQLRD
                     GTTGTIDFDPKPENGEERQTEKTLGDIIARINAAAPGKLQAELSADGKRLVLRDLTEG
                     TNEFKLEALYGSQALHDLGLDVAAEGDTITGQRLIGSLKSPALSQLNGGQGIGTLGLI
                     RVTDRMGQQAVVDLSTADTLEDVIRAINAAEIGVTASVNSARNGIVIQDTSGGTGHLI
                     IESLDDNQSAEKLHIAVNDAVSSKDSGDLHLRVISVNSRLADWNGGQGVSLGRFTVTD
                     SKGVTGTIDLAAIQAQTVGDVIAAINRTAANVIASINETGDGILIRDLANGSGKLVIN
                     DLGGTTAKDLGLLRESTLRDFNGDSDYVIDGSLTYRIELDKNDTLTTLTEKINGLKAG
                     VKASILYDGSTRPYRLSLVGERGGNVAQLVLDDSQLGIDLQRITQARDAQLALGYVGS
                     AGAVVLSSRTSTFSGIVPGLELDVLQGTNTPVNVQVQTSDADLVASVQTFVDNFNKFW
                     KRYKELTKYDTETQKGEVLASDPTAIRLGSEVSYLLSSRFAGAGKIQSLAEIGISINQ
                     DGTLSFDSSALQAKYAEDPEAVKKFFTDATFGFAKKYDKLSESLAGENVSLISQRLNA
                     LSQKYEDNSEKIKWWNEKLERRRQQLLLYFYRLENTIAKLKANLDTLSALQPISYDWL
                     RKSK"
     gene            7412..7957
                     /locus_tag="PLD05_05735"
     CDS             7412..7957
                     /locus_tag="PLD05_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOP76971.1"
                     /translation="MSASVGDSYREGEILTAPPQRLHLMLIEGAIRYTERTRHFWREN
                     QNDAAFESLLRAQQIVSEILAGIKISDEFPLARKVAAIYTFIYRALVQAGLTREEKYL
                     DDALRVLHIQRETWEEVCRRLGGSSPSTDDIRISEAAASVAAKETIRSTPAAPHARPP
                     LTNLGTPDYPQEYATGFSWEA"
     gene            7995..8858
                     /locus_tag="PLD05_05740"
     CDS             7995..8858
                     /locus_tag="PLD05_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOP76972.1"
                     /translation="MPKDVTLIRVGHSPDPDDAFMFFALAKEKIPTGRYRFTHELADI
                     ETLNRRALQGELELTAVSFHAYAFVRKHYILCHCGASMGDGYGPIVVARSSMTVNDLR
                     QTPIAVPGRWTSAFLALRVFLQDEFPFVIVPFDEIPRAVASGSYQGQPIGAGLLIHEG
                     QLTFGQQGLKLIVDLGAWWKEQTGLPLPLGGNAIRRDLGQQVIADVHHLLRESIDYAL
                     SHRDEALAYALNFARDLDRPLADRFVGMYVNEWTRDLGEAGRQSVALFLQKAYESGVI
                     AEPVVPEFVPM"
     gene            complement(9079..9303)
                     /locus_tag="PLD05_05745"
     CDS             complement(9079..9303)
                     /locus_tag="PLD05_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOP76973.1"
                     /translation="MAEGVIKKLVVDRGFGFIQTSGIRKSDVFFHFSAVEGARFEDLR
                     EGQSVEYELSSDAPNPGRGKGPRAVSVRPK"
     gene            complement(9739..11013)
                     /locus_tag="PLD05_05750"
     CDS             complement(9739..11013)
                     /locus_tag="PLD05_05750"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOP76974.1"
                     /translation="MWPALPTWMCWTLLVVGGAIFGAAANWLADALAWQPRRISPFFP
                     TESGRRRRPIEYLPILGWLFRTGDSERHGRFFWIRPLAVELVCAVAIPLLYWWEVIQA
                     GLVPSGVPRPADGVLHAMFARHLLLIFFMLVASLIDWDEKLIPDSVTIPGTLLALILA
                     ASLSTSQLPHGIVHRAVFLGDDVMEFGKPEYSYLTFSAPYAWPKAFGGQPHGHGLSLG
                     LACWWLWCFALMPRRWYRRKGFWKSLKMMCARLYRSRVTGGLVVMGLIGSAGILFTWI
                     VGGSHWQALLSALVGMAASALLAWVVRLVGSMVLDREALGFGDVTLMAMLGAYLGWQP
                     GIILFFLAPFAGLVVAIIILILRRETEIPYGPFLCLGALVTIVFWRPIWGFASRIFEL
                     GFILLLLAAACLVLLALLLLVIRFIRERLFGV"
     gene            complement(11013..11555)
                     /locus_tag="PLD05_05755"
     CDS             complement(11013..11555)
                     /locus_tag="PLD05_05755"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOP76975.1"
                     /translation="MNIVLIGYRATGKSTVARLLAQRLGWQWVDLDVEIERQAGKSIA
                     QIFAEDGEPVFRDWESRVVAEFCGRSGWVIAAGGGAPLREENRKAMRANGRVVWLTAS
                     PETIHRRMTTDPTTGARRPNLTSQGGIAEIIELLSKREPIYRETADWVVDTEGKSPDA
                     VVQEILEHLRPVMDQVGVNP"
     gene            complement(11909..>12301)
                     /locus_tag="PLD05_05760"
     CDS             complement(11909..>12301)
                     /locus_tag="PLD05_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOP76976.1"
                     /translation="ATVTLTDGDAEKARLLAEKLDCAYVPWDDRHSVACDILANCTPV
                     GMHPNVDESPFDAKYLRSHMLVFDAVYNPENTLLIKDARARNCKVVTGVEMFVRQACM
                     QFKLFTGRDGPADLMREVLKRATSAAKW"
     assembly_gap    12302..12401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12402..12629)
                     /locus_tag="PLD05_05765"
     CDS             complement(<12402..12629)
                     /locus_tag="PLD05_05765"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOP76977.1"
                     /translation="MEQHDRRSIICVTIARGRHRHVIAEHRHLAEHGAKLVEIRLDYI
                     NGPVNVKRLLAERPCPVIMTCRRERDGGRFNG"
     gene            complement(12920..14197)
                     /gene="rho"
                     /locus_tag="PLD05_05770"
     CDS             complement(12920..14197)
                     /gene="rho"
                     /locus_tag="PLD05_05770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOP76978.1"
                     /translation="MGRKRRNGAMRSKNGQNGSAAEANASRNGGSASQDTKTTVTAVE
                     TLDSTELHEGGGVLELHPSGYGFLRDPAANYERRMSDPFVPGSMIEKYGLRQGVYIRG
                     LVQPGKKGQGPRLREIHDVDGLPPQEYVNVKNFDELTPINPEEWLRLETGPEPLTTRV
                     MDLLTPIGKGQRALIVAPPRTGKTVLLQHISQAIATNYPEMKLIILLIDERPEEVTDF
                     RRVCKNAEVVASSLDCEIESHVRLSQLIVERCRRLAEMGKDVFLLMDSITRLARAFNK
                     WVGNTGRTMTGGIDVKAMDVPKKLFATARAFEEGGSLTIVATALIDTGSRMDELIFQE
                     FKGTGNMELVLDRRLADRRIWPAIDITQSGTRREEKLLPPDVLRAVTILRRMLSSMNP
                     VDAMEQLTAKLAKYKSNREFIELITGAHANVSD"
     gene            complement(14944..16218)
                     /locus_tag="PLD05_05775"
     CDS             complement(14944..16218)
                     /locus_tag="PLD05_05775"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOP76979.1"
                     /translation="MVWRWSLVEWVTLVMACAGLSSVVCHAEERPYRRISLATYQDKM
                     EAAWIGQMVGVGWGGPTEFRAVGRILREDELPAWKPEMVNQFNQDDLYVEMTFLRTLE
                     EYGWEVDIRQAGIDFANSRYRLWHANRAGRDNLRSGIAPPDSGHPQFNKHADDIDYQI
                     EADFSGIIAPGLPNTVIRLGNIFGRLMNYGDGLYGGQFVGGMYAEAFFENDPLKIVEA
                     GLRCIPAESQYAECIRDVIAWWKENPQDWQATWQKIEEKYNRNPRYRRASCMKELGNI
                     DAKINGAYIVMGLLFGKGDIDQTVIISTRCGQDSDCNPSNAAGILFTTIGAKQLPERF
                     TSKLNREAVFSHTAYNFDRLVEVCTKLAREAVQRAGGKIEKDDQGQEVWLIPVEEPVP
                     NALEQCWEPGPVANSRFTPEEQQKILYPPGEQ"
     gene            complement(16675..16998)
                     /gene="rpmB"
                     /locus_tag="PLD05_05780"
     CDS             complement(16675..16998)
                     /gene="rpmB"
                     /locus_tag="PLD05_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOP76980.1"
                     /translation="MSQKCEICGKTPAIGKRVTTRGKAKYLGGVGTKITGISPRQFRP
                     NLQRIRITDENGAPRRVRVCVQCIRSGRVTKRIKNRPFALPADAAALLAAAREKKAQK
                     KGKKS"
     gene            17891..18289
                     /locus_tag="PLD05_05785"
     CDS             17891..18289
                     /locus_tag="PLD05_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOP76981.1"
                     /translation="MSDFNLPEPGAEQQEEKTAQQPTPEAQAQQPAGPPRVTVQIDDS
                     KIVASYANFCRVTGTPEEVIIDFGLNPQPIGIPSQPIAVSQRIVMNFYTAKRMLHALQ
                     LTIQRHEATFGVLETDIQKRVRPMPPRPNA"
     gene            18917..19975
                     /locus_tag="PLD05_05790"
     CDS             18917..19975
                     /locus_tag="PLD05_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76982.1"
                     /translation="MTARQMRRHANEDQGGSAPAPGPDALASGSPSVAQAGSPENQEE
                     LPPGVVATAEWLVSFCHEALLQQQTPEALAARAYLQMQGLAWSGIADLPIGVLNDASK
                     ARDIYQDRQMGSVEDIEYWLSDARLSRVLLGPIRDGRGKLVTLWARPVEPTQRTLLYR
                     RPWQERVAVYGAECLSTVDPVPVYVVEKILDALVLRSHKIQPAVAFGRRFDQVPAQSW
                     AWLGRNVAGPIVLIPVGSHIPSTIFRRVRIQVERLIDPPEIWVLPPKRMFAPLGRMAA
                     VLKSPEFQEFIRDRAVPLLGRKRTIQLTQGVAAMHRQSASTGPARYLGTTQEGPKIDP
                     NQRPKDLRPGSPDWLAFD"
     gene            20754..22352
                     /locus_tag="PLD05_05795"
     CDS             20754..22352
                     /locus_tag="PLD05_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP76983.1"
                     /translation="MALYEADHLFDELELRSMEDEEELEFQGRGLLEMAESYADDELA
                     EDEEAISEEAYQAAANLEESEEDTILTADEEESWSDDPVRMYLTQMGEIPLLSRQEEI
                     ALAKKIEITRAKFRRKLMECDYVLQQAVRTLARVDEGKLPFDRTVQVSVTDHLEKEQI
                     LGRLPHNLRTLQAILKRNRQDYLTALSKSKPMEERRAAWRRLGRSRRRAGRLAEELGL
                     RTQRLEPMIKALEEFSRRVDELKARIQEHANAGRPLEERKPWIKEYRDILRLTQETPT
                     SLRNRVNYVKKIYAEYQEAKRALSEGNLRLVVSIAKKYRNRGLSFLDLIQEGNAGLMR
                     AVDKFEYRRGFKFCTYATWWIRQAITRAVADQSRTIRIPVHMAETMTKVRNASRRLYQ
                     EKGHEPTLEEMAAAAEAKTEETRRVLTMNRYPISLDRPVGNSEDSHFGDLLPDTSAES
                     PLDGATHDMLREKINKVLRTLSYREREIIKLRYGLGDGYCYTLEEVGHIFKVTRERIR
                     QIEAKAVRKLQQPSRSQELVGFLD"
     assembly_gap    22353..22452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22694..23374
                     /locus_tag="PLD05_05800"
     CDS             22694..23374
                     /locus_tag="PLD05_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76984.1"
                     /translation="MVKHSWLLFLWPGMAALWVRPTLTAWLGSAAAAVVFHLALAATI
                     LWPEWLSPQERMLVWIALGVAWLSGVAWSYQFLRREEARQHRRPAEDRFPAATEAYLR
                     GNLAAAESLVREILRECPRDVEAALLLATIWRRREQFDAAEKLLRNLARLDLAIPWLW
                     DIERELLRIQKSREEKADEPSPQPEEEQTPVEEPVAGEPPPSSQKTVPLPERVPAGRT
                     AQPATKAA"
     gene            24168..26720
                     /locus_tag="PLD05_05805"
     CDS             24168..26720
                     /locus_tag="PLD05_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOP76985.1"
                     /translation="MQLANQEAQRFNHEYVGTEHILLGLIKEGSGVAANVLRNLDVDL
                     KKIRLEVEKLVQTGPDMVTMGRLPLTPRARKVIEYATEEARNLNHNYIGTEHLLLGLL
                     REQEGVAAQVLMNLGLKLEEVREEVLNLLGHGSEAAETPDRNAGLGQDEGTGEPSARA
                     SRSRTPALDSFGRDLTELARQGKLDPVIGREKEIERTMQILCRRTKNNPVLLGEAGVG
                     KTAIVEGFAQRIVKGDVPEILLDKRVVVLDLAMMVAGTKYRGQFEERIKAVMNEVRRA
                     GNVILFIDELHTLVGAGGAEGAIDASNVLKPALARGEIQCIGATTLDEYRKYIEKDSA
                     LDRRFQVVMVEPNTKEETIAILKGLRDRYEKHHHVQITDDAITTAVELSSRYITGRCL
                     PDKAIDVIDEAGARVHLKSLTRPPDLKEIDEEIERLNKEKEEAVANQDFEKAAKLRDQ
                     ADKLKKKKANMMHEWRERSASVDGIVDAEVVAEVVSKMTGIPLTRMTTEDSIRLLKME
                     EELHKRVISQDEAIKSICKAVRRSRSGLKDPKRPVGCFIFAGPTGVGKTLLAKALAEF
                     MFGDEDALIQIDMSEYMEKHNVSRLIGAPPGYVGYEEGGQLTEKIRRRPYSVILLDEI
                     EKAHPDVFNMLLQVMEEGRLTDSFGRNVDFRNTILIMTTNAGAEAIKNEAAFGFQKPD
                     DDTSYENMKRRVIEQIEKVFRPEFLNRVDDIIVFRHLTQKDLFDVVELELGKVRSRLA
                     ERGLSLVLTEEAKKLIIKRGTNLDFGARPLRRAIENYIEDPLAEELLKGEFEGKDTIV
                     VKVKDVGGKKQLVFEGVVGQPDLSSEGETSGDQQPEVAGVSGGEGSPSPSQE"
BASE COUNT         5862 a         7683 c         7551 g         5441 t
ORIGIN      
        1 gcatcggccg gtcggataat ctgcaccgcg acctgggttg atccccccaa gggctgggcc
       61 tgatagattt cggccgtggc gcggcctgtg gaatccacgg ggacttccac gctcgtggtt
      121 ccgtttggcg cgaacagcgc gctgacccca ccgatgatct cgtaacggac cttccaccca
      181 gcgcaaggtg aacgatcgcg gaggcgggtc accgtggtcg ctagctgatg gcgagaaccg
      241 gccggcacaa tggcgggcgg tgggagcgtc cattgggcat caacccaatg aatgaagacc
      301 gtctggaccc gacgatccca gctatgcaca catggaacgt aaacgttgat ggccgtcgtg
      361 ccttccaccg ggctggtgac gctgacccaa gcctgccccg ggcgaacgat gacgtcgtca
      421 ttcggggcga ccgttcctcg agtcagccgc aggtattcgc gggaagtgct ggtgatgacg
      481 taatgttcgg tgatcttgcg gggccgggta aaatccccaa caagatagtc catgaaggtg
      541 ccacgtctaa aatcgacaaa atgcccaaca ccgccgggcg tgatcgtcca ctccacccgc
      601 tggtttgtca gcataaactg gtcctgcccc cgcacactcg ccaccaggat gacttcgctg
      661 ccaacggcgg cagccgtctc ctgaggggtc accatgaatt cgctgcaatc cccggcaagg
      721 atgggcccgg gaacttcgcg gtagtatccg ccagactcat ctgccggagg caagacgggc
      781 gcaccgggcg cggtgttggt ggcaggcgca acacaaccgt cggctgccaa gatcacgaca
      841 agaagcatcc cggcagcggg gccgaatctc caccaaatgc ggtcgttaac ccatttcctc
      901 atgggtactg ctatggaatg gccggtctcc ccaatccggt caatgcattg ttaaacctcc
      961 ggggaccgtc acataaagga cacaaggcac gcgccagcct caccaaggcc accgtcgcgg
     1021 agcaggttga cccctcatgg caacggtcaa aggtccaccc attagggaga atcgtaccca
     1081 gcccatccca gtgattacgt caaatccccc tggcgaatgc caaccctgcc ctggtgacac
     1141 acacttttgg ggatgataag ggattttcca agtttcttga agaccggttt ttgtcgctct
     1201 gcccgaaagg ggggatacag gcggatcgac aattggtacg ccgggggaca tgaaggcgcg
     1261 tgcccctcca aatcccgtgg aacacgtcag gcaaaaaaac ggggcaacca caagggttgc
     1321 ctctgtgggg atggcctctc tcgctggttg acaagagggg atcgcccttg gcgagttacc
     1381 taaacgcggc caagctggac caatttggca aaatgctcaa aagaccggtc ataggtcgcc
     1441 tcggcatcgc gggggaagca acatcctggc aattgccggt tcttaaggtg gtactgaagg
     1501 cagtcgcaac atatcccttt ccgggaacaa ggctcatagg aacaggtaca aatcgtcagg
     1561 ttccgttctt tcttgcattc catcggaaaa ctccttcttc accaggtcat cgcgttgcta
     1621 gacaaacggt ttcctctttt cttacgcggc tacttgccga tgatcaaggg gcttcccaca
     1681 ccgcgttgtt tggagctctt gtcacgctca ttggcaccgg aaaagtgaca aatgaaaaga
     1741 cctaccacca aaacgttatc tcccagcaag gagcgtgcgg taatctctcg cccggcatgt
     1801 tcgtccgcaa cgcgcaaaga gcggcctgcg ctttagaaag gcctgggata gtgagtcggc
     1861 aagctccgca aaattgccca ccgctagtga tgctgcctat cgggcacgag gatgggcctt
     1921 ttcatagacg gctttcaggt tggctgtcgt tagatgcgta taaatctggg tcgtcactag
     1981 gcttttgtga ccgaggagct cttggacgct gcggatatcg gcccctgcgt taagcaaatg
     2041 cgtagcgaag ctgtgccgca gtgagtgggg cgtggtccgc tgatcgaggc cggtttggat
     2101 gaggtatttt tccagcatcc gcgcaacact ccgcgttgtc aggcgaagct tgaagcgatt
     2161 gacaaaaagg ggtcggtcag gattatccac aaacgagggg tgtatctccc gtaccgccag
     2221 ccaacgacgg atggcacgca cagcgtggga gcccagcggg gccagccgct ccctgcgccc
     2281 ttttcctcgg acgcggacca cttccgcccg caaatcgaga tcggaatcgt tcagtcccac
     2341 gagttcgcta atacgcaggc cagcagaata gagagtttcc aggatggcgc gatctcgtag
     2401 gcccatcgga tcgttggcgg gcggggcctc caagagtcgc cggacctctt ctgtcgaaag
     2461 gacgtggggc aaattcctcg gcttgcgagg attgcggagg ggcttcgcgg ggttgtcttt
     2521 tacccagcct tcccgcatcc caaagcgaaa gaagcttctc agactggcga gacgcctcgc
     2581 gacggtggca aaggagtaat tggcctcgtg catggccgcc acatagccgc ggagatcgag
     2641 tgtcgtaatc tgactgggag aaggacaacg cccttgatag gcctcggtca ggtattctag
     2701 cagagctgtg aggtcttctc gataactctt gatcgtcagg ggcgaggcgt ttttctcaac
     2761 cgcaaggaat cggagaaacc tcgccatcgc cgtctgcatc gtcaactcgc ccgatgagcc
     2821 ttgcggtcct ctgtaatgat gattgttcat cgagaaggta cagcgtcaat gtctctgccg
     2881 ggctaaaaga tgtcctccgg atcggcaggt tgcggggcct taaagcggtt ttggagggca
     2941 cgtcgtcggg cctcttgacg aactttctga ctgagaaccg cggcatcgta ccaactgaaa
     3001 ctcaactccg gattggccgc ataacgtccg agcgtgcgga tcatctccag acggttatca
     3061 tcgtcgtaaa gaaagatgta ccgctcctgg cctttgatca atgcgatgac gttaatttct
     3121 cggctcacga tcaggcgtct cctgcggtca atagctgcga gcttgagttc ctgcctgcac
     3181 caattctacc caaattgaat gccttattcc ttttatcggc cggatgtcgt gctccaaact
     3241 agttcacttt cgtcggcccc acccctggcc gttagcattt cccaaatgtg aacccggcag
     3301 caaaaccgga gaaactcttc gctttttgtg agatatgact gccatccccc caatcgggca
     3361 tcgtcccgaa acttcgcata gtcctgaagc aatttgacca gttggctatc gcccccgttg
     3421 taaacccgtg tgtgcttcat gaagggctct tcgcccgctc cgcaagcaga atctcctgcc
     3481 tgggcaagtt tggggatgag cccctcgggc gagagcgaag cctggctttt tccctcccct
     3541 gtctgtggaa tttcgaagcc cggctgcgca gcggcatctg tcgcggaagc atccgcagct
     3601 tggggcgact ggttccgcag ggcctcgcgg gcataggcta gctgtgactc caggaccagc
     3661 cgggagggga tatctttttc ctccttcgtt ccccatggca aaccgtagcc tggtggaacg
     3721 gggcaaaagc cggggtttgc cgcgtaccat tccacctgaa gcccacatcg cgggggatag
     3781 taaggtgaga attccgtcac ggccccaatc accacagcat cggcccccag caactgggca
     3841 agatggcgga catcttgggg attattgaga ctgatgcggt gctggaacat tgcccgttcg
     3901 accaccccca cagggatgac ctcaaagccg ggaacctctt gaagctcgtt gtagtaagca
     3961 atggccacct tccgcccgtc caaagtcgga tctgtcgaaa gattgaaaaa cggggctaca
     4021 gcaatcttgt ggatttgagg aaaaggattg tgaacaaggg gccgccattc cggcttctgg
     4081 aatagcgagc aacctgcggg aaacacgagg actgtcaaaa tcaatgccac cgataaccgg
     4141 cgatccaaca tccaaggggc cgaatgggac cctcccaacc gggtgaatcc ccggggtatg
     4201 tcggccgtcc tttgttgttg gtaaagcacg tttgccattg tgagccgaac tggtccaatc
     4261 tcgattgttc ccgcaaatct gatagggccc tgcctccggc acaatcgacc ctgccggcgc
     4321 aagccgttct gcccaacgcg attgacttat cggcaatgtt cccgcagatt ctccaaacat
     4381 ttttattggc aaactcaacc aaaaacggtg gctggacgta gtcgtctgtt ttgaatttcc
     4441 catcttttgc attttaacga acagcgtggg aactaggttt tcttatggga agggtcttcc
     4501 tcaatgcagg ccgccgtgat ggtcgatgtc aaaaaaggcg gactttgcca acgggtgaaa
     4561 ttgtgcgcga ggacttcgca tgggccggat tcagaccaat gtcggcttga taacgggcgt
     4621 ccccattggg gagaccgtca atcagcttat gttgattgag tcccgcccaa aggagaacct
     4681 cgagtcggcc aacaagaaaa tcgacgccga gcggacggcc atcacggaac tttccgtgct
     4741 ctttttggca gctcagtacc ccatcaaaaa tcttttgaaa gacgccgttt acaccaagcg
     4801 gtccgccacg agcagcaatc cctcggcgtt gaatgtccgt gtcaccggca ccccaagcgt
     4861 cggtacctat gtgttcacac cgatccgtac ggcgcaatcc gagcagtggt tatcaagcgg
     4921 cgtgcgggag agcacaacgc ccctgggcgg gggcgtgtta tcttttcgtt atggcccaag
     4981 tatcgatcga ccattggctt tggatgcaat tcgtggcggc caaggctttg agcgaggcgt
     5041 catccgcatc accgaccgaa gtggcgcctc cgcggaaatc gacctgacca ccgtgcaaac
     5101 gcttgacgat gtgattgagg ccatcaatag gaacatgcgg atcaacgtct tggccgaggt
     5161 ggtgggcgac cacattcgcc taaccgaccg cacggggatg acggcctcca atctccgcgt
     5221 gcaggacttg ggcaatgggc gagcggcggc ctccttgggt ctggcgggga tcgacgtggc
     5281 cgaatccact gccgacggaa gcgacatcgt ttggctaacc accgacacac tcttgagcga
     5341 cctcaacgac ggactgggta tttccgccag ttcaacgttg cccgacattt ccttccaatt
     5401 gcgagatggg acgaccggga cgatcgactt tgatcctaag ccagaaaatg gtgaagagcg
     5461 acaaacagag aagacgctgg gagatatcat cgctcgcatc aatgcggcgg ccccgggtaa
     5521 gcttcaagcg gagctgtcgg ccgacggaaa acgacttgtc ctccgcgatc ttacagaagg
     5581 aactaatgag ttcaaattgg aggccctcta cggttctcag gcccttcatg acctggggct
     5641 tgatgtggcg gccgagggcg acaccatcac cggtcagcgg ctgatcggtt cgctcaaatc
     5701 accggccctt tcccaactca acggcggtca gggaatagga acgttgggac tcatccgtgt
     5761 gaccgaccgc atgggacaac aggccgtggt cgatctctcc actgccgaca ccctggaaga
     5821 tgtcatccgg gcgattaacg cggcagagat cggcgtcaca gccagcgtga attcggcccg
     5881 caatggcatc gtgatccagg ataccagcgg cgggacgggt catctgatca tcgagagcct
     5941 tgacgacaac caatcggcag agaagttgca catcgctgtg aacgatgcgg tatcctccaa
     6001 ggacagcggc gaccttcacc tgcgagtgat ttccgtcaat tcccgcctgg ccgattggaa
     6061 cggtgggcaa ggcgtatccc tcgggcgttt taccgtcacc gacagcaagg gtgttaccgg
     6121 gacgatcgat ctggcagcga ttcaggccca gacggtgggc gacgtgatcg cggccatcaa
     6181 tcgtacagcg gccaacgtca tcgccagcat taacgagacg ggcgacggca tcctcattcg
     6241 ggatttggcc aacggttcgg gaaagctggt gattaacgac cttggaggaa ccacggccaa
     6301 agacttgggg ctacttcgcg aaagcacact acgcgacttc aacggtgaca gcgattacgt
     6361 cattgacggt tccctgacct accgcattga attggacaaa aacgatacac tgaccaccct
     6421 tacggagaag atcaacggcc tcaaggctgg tgtcaaggcg agtatcctct acgatgggtc
     6481 aacccggccg taccgcctgt ccttagtcgg cgagcgcggg gggaatgtgg cccaactcgt
     6541 cctcgatgat tcgcagttgg ggatcgacct ccagcggatc acccaggcgc gggacgcaca
     6601 attggccctg ggatacgttg ggtccgccgg ggcggtggtg ctttcctcac gaacgagcac
     6661 cttctcgggg attgtgccgg gattggagct ggacgtgctc caggggacga acacaccggt
     6721 caatgtccag gttcaaacga gtgatgccga tctcgtggcg agtgtgcaga cattcgtcga
     6781 taatttcaac aagttttgga agcgctacaa ggaactgacc aaatacgaca cggagacgca
     6841 aaaaggcgag gttctggcct cggatcccac ggccatccgg ttagggagcg aggtctcgta
     6901 cctcctcagt tcgcgttttg ctggtgccgg gaagattcag tcgctggcgg agatcggcat
     6961 ttcgatcaat caagatggga ccctaagctt tgatagctcg gccttgcagg caaaatacgc
     7021 tgaggatccg gaggccgtca agaagttttt caccgacgcc acgtttggtt ttgccaaaaa
     7081 atatgacaaa ttgagcgaga gcctagccgg tgaaaatgta tcgctcattt cccagcggct
     7141 caacgccctt tcccaaaagt acgaagataa ctctgagaag attaaatggt ggaatgagaa
     7201 gctagagcga cgcaggcagc aacttttgct gtatttctat cggttggaga acacgatcgc
     7261 caagttgaag gcgaatttgg acacgttgtc ggcgctccag cccatttcct atgattggct
     7321 ccgcaaatcc aagtgagatt gatggagcca cttagaccca acatcaatcc cgcatgataa
     7381 ccttggagat ttgccggaag ggactctgcc tatgtctgct tcagtgggtg atagctaccg
     7441 cgaaggcgag attctcacgg cgccacctca gcggctgcat ctgatgctca tcgaaggtgc
     7501 catccgttat accgaacgca cgaggcactt ctggcgagag aaccaaaatg acgcggcctt
     7561 tgagtcgctc cttcgtgccc agcaaatcgt cagcgaaatc ctcgcaggca tcaagatttc
     7621 ggatgagttt cccctcgcac ggaaagttgc ggccatttac accttcatct atcgcgcgct
     7681 cgttcaggcc ggtttgaccc gcgaagagaa gtacctcgac gacgccctcc gggtccttca
     7741 catccagcgt gaaacgtggg aagaggtctg ccgtcgcctt ggtgggtcct cgccttcaac
     7801 ggatgatatc cggatcagcg aggccgcggc ttctgtagcg gcaaaggaaa cgatccgcag
     7861 taccccggcc gcccctcatg cccgcccacc cttgacaaac ctcggcaccc ccgattatcc
     7921 ccaggaatat gccacggggt tttcctggga ggcctagcag gccacaccct cttgccgcgt
     7981 gagaggattt cctgatgccg aaagatgtca cactgattcg ggtaggacat agccccgatc
     8041 ccgatgatgc cttcatgttt ttcgccctgg cgaaggagaa gattccgacg gggcgctatc
     8101 gttttaccca cgagttggct gatatcgaga cgcttaatcg ccgggcgctc caaggcgaac
     8161 tggaattgac cgccgtcagc ttccatgcct atgcctttgt ccgcaagcat tacatccttt
     8221 gccactgtgg ggcaagcatg ggggatggct acggccctat cgtggtagcc cgatcgtcga
     8281 tgaccgtgaa tgatctgcgg caaacgccga tcgccgttcc cggacgctgg actagcgcct
     8341 tccttgccct tcgcgtcttc ctgcaagatg agtttccctt tgtgatcgtc ccctttgatg
     8401 agattccccg cgccgtggca agcggtagct atcagggaca acccatcggt gctggactgc
     8461 taatccacga aggccaactg acctttggcc agcaggggct gaagctgatc gtcgatctcg
     8521 gggcgtggtg gaaggaacaa accggactgc cgttgccgct cggcggaaat gcgatccgcc
     8581 gcgaccttgg acagcaggtg atcgccgatg tccatcacct gctccgcgaa agcatcgact
     8641 acgcgttgtc ccaccgcgac gaggcccttg cttatgccct aaattttgct cgtgacttgg
     8701 acaggcctct tgccgatcgt tttgttggta tgtatgtgaa cgagtggacc cgggacctcg
     8761 gcgaggcggg acggcaatcg gtcgcgctct ttctgcaaaa ggcttacgag tcaggagtca
     8821 ttgcggaacc ggtcgtcccg gagttcgtac cgatgtgagg gaagcctttc gagaaaccgg
     8881 cgcgcaaaaa aaacagggag cccttgctcc ctgttttcgt catctatcga cgataaaact
     8941 ccagcttact gctcgacgtc aagccagatg tcatctgccc aactgatcct gagtttacac
     9001 gagcggatgc gacggggcag ccgaaaaaga tgtgaagcca gccatgagct tcacggcgtc
     9061 tcacacggag actaaggact acttaggtcg cacggagact gcccgggggc cttttccccg
     9121 acccggattc ggtgcgtccg agcttaactc gtactcaacg ctttgacctt cgcgaaggtc
     9181 ttcaaagcgg gcaccttcca ctgcggaaaa gtggaaaaac acatcggact tccggatgcc
     9241 gctggtttgg atgaaaccaa aaccacgatc cacgaccaac ttcttaataa caccctcggc
     9301 cattaaagcc actcctcaaa aacgtaacga gcggagttac ctccgcacac acaaaacaac
     9361 tcggactgtc tcatgtcgtg agcaacccgc tcgttggccg ttagaccaag gagtggctca
     9421 gctaacacaa aaccatccga actcccaaag aatgtcccgg atcgatcttg gggcccaccg
     9481 gtccattctc acgacaacta aaactctagc ctaaagacag ccttcaggca agaggcatct
     9541 ttccgtttta tcggaatatt tcactttttt ccgaacccct cttttgtggg ttttgctcat
     9601 caaaaccgct caccccgttg ttttcgcctc agtgagcatc ttttcaacga tttatttcgc
     9661 attcgccaaa cgcgcccata aatttgctca catttctcca cccttcgatg gtgagcaacg
     9721 acttttcgca gaatgagact acacgccaaa aagtctttcc cgaatgaacc ggatcaccaa
     9781 tagcaacaag gccagcaaaa ccaggcaagc cgccgcgagc aggaggagga taaaccccaa
     9841 ttcaaagatt cgggaggcaa atccccagat aggtcgccag aagacgatcg ttacgagcgc
     9901 cccgaggcac aaaaacggcc cataggggat ttctgtttct cgtcgcaaga tcaggataat
     9961 gatggccacc actaacccgg cgaaaggggc caggaaaaat aaaataatcc ccggctgcca
    10021 accaagatac gccccgagca tcgccatgag tgtcacatcg ccaaagccga gggcctcacg
    10081 atccaggacc atggaaccca ccaggcggac gacccatgcc aagagggcgc tggccgccat
    10141 ccccaccaag gccgacaaaa gagcctgcca atgcgatcca ccgacaatcc aggtgaaaag
    10201 aatccctgcc gagccaatca gtcccatcac caccaggccg cccgtcacac gcgaccggta
    10261 gagccgcgca cacatcattt tgaggctctt ccagaaccct tttcgccggt accaacgtcg
    10321 gggcatgagg gcaaaacacc atagccacca gcaggccaaa cctaacgaaa ggccatgtcc
    10381 atgtggctgg ccaccgaagg ccttcggcca tgcatagggc gcactaaacg taaggtagct
    10441 atattcgggt ttgccaaatt ccatgacgtc atcaccgagg aaaaccgccc ggtgcacaat
    10501 tccgtgggga agctgtgacg tcgaaaggct agccgccaga attaaggcga gcaaggtccc
    10561 cgggatggtt acggaatccg gaatcagttt ttcgtcccaa tcaatgagtg aggccacaag
    10621 cataaagaag atcagcaaaa ggtgccttgc aaacatggca tgcaaaacac catcggcagg
    10681 gcgtggcaca cccgagggga ccaatcccgc ttgaatcact tcccaccaat acaaaagggg
    10741 tatggcaacg gcgcagacga gctccacggc cagcgggcga atccaaaaaa agcgaccatg
    10801 gcgctcacta tcccccgtac ggaaaagcca gcccagaatg ggcaaatact cgatcggtct
    10861 gcggcgcctc ccggactccg tggggaaaaa cggcgaaata cggcggggtt gccaagccaa
    10921 ggcatcggca agccagttgg cggcggcccc aaagatcgcc cctccaacta ccagaagggt
    10981 ccagcacatc caggttggca aggcaggcca catcacgggt tgactcccac ctgatccatg
    11041 acgggtcgga ggtgctccag aatttcctgg actacggcat ccggtgattt tccttcggta
    11101 tccacgaccc agtcggccgt ctcccggtag atgggctcgc gcttgctgag gagctcaatg
    11161 atttctgcga tccctccctg gctcgtgaga ttggggcggc gggcaccggt tgttggatcg
    11221 gtggtcattc ggcgatgaat cgtttcgggt gaggccgtca gccacaccac tcgcccgttg
    11281 gcccgcatcg ctttgcgatt ctcctcgcgt agcggtgcac cgccaccggc agcaatgacc
    11341 caaccggagc gtccgcagaa ttccgccacc acgcggcttt cccaatctcg aaagacgggt
    11401 tcgccatcct cggcaaagat ttgggcgata gacttaccgg cttgccgttc aatctccaca
    11461 tccaaatcca cccactgcca acccaatcgt tgggcaagaa gccgggcaac cgtacttttc
    11521 cccgtggcgc gatacccgat gagcacaatg ttcataactt gggcaaagat aggaaagccg
    11581 attcatcgtg tgtcaaaatc tcattttccg cttgtctcca tcatactgat cggacgagct
    11641 ttgcgaaaga tttgtttact gcggcttgcc gcctgacaac acgctcttgc cagccatggc
    11701 ttgaaagcct atctgataat ctcccctcgc ccgttcggcg ggagaggggt cgggttgaga
    11761 gtagctcgaa tcgctgccgg ctttcttaat cgattcgacg ttcgcttgcc ctcaccctaa
    11821 ccctctcccg aagggagagg gcgacttttt gggataggct cttgagagcc tacgatcggt
    11881 tgcggcacgt gttcctacgg aggccgcgtc accattttgc ggcggaggtg gcgcgtttga
    11941 ggacttcccg catcaaatcg gccggaccat cccgcccggt aaacaacttg aattgcatgc
    12001 acgcctgccg cacaaacatc tcgacgccgg tgacgacttt gcaattccgc gcgcgggcat
    12061 ccttaattag cagcgtgttt tcgggattat acactgcgtc gaagacaagc atatggctcc
    12121 gcaaatactt ggcatcaaat ggcgattcat ccacgttggg atgcatcccg accggtgtgc
    12181 aattggccag gatgtcacaa gccacagaat gtcgatcatc ccacgggaca taggcacaat
    12241 cgagtttctc cgcgagcaat cgggcctttt cggcatcgcc gtcggtcaat gtgacggtgg
    12301 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgttgaa ccgaccgcca
    12421 tctcgctccc gacgacacgt catgatgacg gggcacggcc tttcggcgag aagccgcttg
    12481 acgttgaccg ggccgttaat gtaatccagc cgaatctcaa ccagtttcgc cccatgttcc
    12541 gccaaatgac gatgctccgc tatgacgtgg cgatgacggc cccgtgcgat ggtgacacaa
    12601 atgatactcc ggcgatcatg ttgttccata ataggccctc ataggatcag acgaacacaa
    12661 cgtggaccgc gttggcttcg atacgtcgct tctccccgtg cgtgctacga cgcttgtgcc
    12721 tttccagact ctcagatggc catgctcttt tctcgcccat cgaccgggga acggcccatc
    12781 tcatttatgg tacacttggc gcgaaccgtt caccacctga ctcgggagaa ggatgcctca
    12841 ccaggaagcc gtcatgatcg tatttgcgaa cgagagatgc acgctagact cgatgccagg
    12901 tcgaggactt tccctcacat caatcactga cgttggcgtg cgcacccgtg atcagctcga
    12961 tgaattcccg attggacttg tacttggcca acttggccgt caactgttcc atggcatcca
    13021 cgggattcat gctggacagc atccggcgga ggatcgtaac ggcccgcagc acatccggcg
    13081 gcaagagctt ttcctcacgc cgcgtgcccg attgggtaat atcaatcgcc ggccagatac
    13141 ggcggtccgc caaccggcga tcgaggacca gctccatgtt gcccgtgcct ttgaattcct
    13201 gaaagatcag ttcgtccatg cggctgcccg tgtcgatcaa ggccgtggcc acaatggtca
    13261 gtgagccgcc ttcctcgaaa gcccgggccg tggcgaacaa cttcttgggc acatccatgg
    13321 ccttgacgtc aataccaccg gtcatcgtcc gaccggtgtt tcctacccat ttgttgaacg
    13381 ctcgggccaa ccgcgtgatc gaatccatga gcaggaagac atccttcccc atttctgcca
    13441 aacggcggca ccgctccaca attaactgag acagtcgcac gtggctttcg atttcgcaat
    13501 ccaggctgct ggccacaact tccgcgtttt tgcacacccg acggaaatcg gtcacctcct
    13561 cggggcgttc atcgatcagc aagatgatca acttcatttc cggataattc gtggcgatgg
    13621 cctgactgat gtgctgcaag agcaccgttt tgccggttcg cgggggggcc acgatcaagg
    13681 ccctttgccc cttaccaatt ggcgtgagaa ggtccatcac gcgggtggtg agcggttccg
    13741 gtcccgtctc cagtcgcagc cactcctcgg gattgatggg cgttaactca tcgaagttct
    13801 tgacgttgac atattcctgc gggggtaggc catccacgtc gtgaatctcg cggagtcgtg
    13861 gtccctgtcc cttcttgccc ggctggacca gacctcgaat atagacccct tgccggagcc
    13921 catacttctc aatcatgcta cccggcacga agggatcact catccgccgc tcgtagttgg
    13981 ctgccggatc acgaaggaac ccgtagccac tggggtggag ctccagcacg ccaccaccct
    14041 cgtggagttc cgtgctgtcg agcgtttcca ccgcagtgac cgtagtcttg gtgtcttgcg
    14101 atgccgaacc gccgttgcga gaagcatttg cctcagcggc agagccattt tgtccgttct
    14161 tcgaccgcat agcaccgtta cgacgttttc gacccattga atcaccgaaa gaaaaaggat
    14221 tcttctcccc tacaaagctt gttaaaagag caactgacgc atgtctgcca agaaaatcat
    14281 tttcctttta gcgcagcctc atgccgacga aatcatcaag aaaggccccc actaccgtcg
    14341 gcgaaaggct ctcttaaaag gaatgagcac gcaacgccat tgacggtctc gtcagaccaa
    14401 gcgcttgata gctgctttca ccaaatccaa taacgattct tgtaagccca ccggtagccg
    14461 cgttgtgcgt ttcgtcgttc gcagcgatgt gttgatcgac cggaagtcgc ccagcgaaat
    14521 gaacgaatca acaccgagct gtctttgtcg agccgcctcg atcgctcaag ttcttgagaa
    14581 acgcctacgg cgcttcagcc gtgtttctca caaagagaga gggaaaaatt tcagaaaccc
    14641 ctgatttccc aatggctacc cgacgcttag agccttacca ctacaatcat atcgtaaggc
    14701 cccaccgtgt caagacagct tccgcccttt acacaagttt aacaaaactt ttctttggtg
    14761 cgctttctcg gcgactgaat cgtttgtgac ggctgtcccg attgcgccag ccatccccca
    14821 gccccgatat ccgaggacgg acgggcgcaa cctagttccg gctaccttca ttatcgactc
    14881 aacggtttcg ctagatttat ttttcgtcgg ccgctgccct gccgcggaac caactagttc
    14941 ctctcattgt tcacccggtg gatagagaat cttttgttgc tcctccggtg tgaaacggct
    15001 gttggcgacc gggcccggct cccagcactg ctcgagggcg tttggcactg gctcttccac
    15061 gggaatcagc cagacctctt ggccttgatc atccttttcg atctttcctc ccgcccgctg
    15121 gacggcctct cgcgccagtt tggtgcagac ctcaaccaac cggtcgaaat tgtatgccgt
    15181 atgactgaac accgcctcgc gattgagctt gctcgtaaac cgttcaggca gttgcttggc
    15241 cccgatcgtg gtaaagagaa ttcccgccgc gttcgaggga ttgcaatcgg aatcctgacc
    15301 acaccgggtg gaaatgatca ccgtttggtc aatatcgccc tttccaaaaa gcaaccccat
    15361 cacgatgtag gcaccattga tcttggcgtc gatgttcccc aattccttca tgcaggacgc
    15421 acgccggtat cgcggattgc gattatattt ctcctcgatc ttctgccaag tggcctgcca
    15481 atcttgcgga ttctccttcc accaggcgat gacatcgcgg atacactcgg catattggct
    15541 ttccgcgggg atgcaccgca gaccggcctc gacgatcttt aaggggtcat tttcaaaaaa
    15601 ggcctccgca tacattccgc ccacaaattg cccgccgtac agcccatccc cgtagttcat
    15661 cagccgtccg aagatattgc ccagcctgat caccgtgttg ggcaatcccg gggcgatgat
    15721 gcccgaaaaa tcggcctcaa tctgatagtc gatgtcgtcc gcatgcttat tgaactgagg
    15781 atggccagaa tctggcgggg caatgcccga tcggagattg tcccgtcccg cccgattggc
    15841 atgccacagc cgatagcgac tgttggcaaa atcgatcccc gcctgccgaa tgtccacctc
    15901 ccagccgtac tcttccagag ttcgcaaaaa ggtcatctcg acatacaaat catcctgatt
    15961 gaactggttg accatttcgg gcttccacgc cgggagctcg tcctcccgca ggatccgccc
    16021 caccgcgcga aattctgtgg ggccacccca ccccacaccg accatttggc caatccaggc
    16081 tgcctccatt ttgtcttgat acgtcgccag cgaaattcgc cgatacggcc tttcctccgc
    16141 atgacagacc acgctcgaaa gccccgcgca cgccatcact agggttaccc attcgaccaa
    16201 cgaccatcgc cacaccatgg aaagtcctcc cttggtttgc acaggctcac cttgcagaca
    16261 caacccccac cgagcggctg acttcggcag gaccgccgat ttccttccga cggcggtcta
    16321 ttatatgccc ctcgggcagc caccgcaaac gactcagtgg taggcaatcc cctagaggcc
    16381 cagcccactt tgacgatccg cccggaacgg atgaacccag cggcgtggcc ggccgagagg
    16441 gcacgtcacg cttccttcaa caccgcgatg gaaaaagcgg aggcctaaag gcgactatct
    16501 gagaaggaaa ccgcggcagt cgccaagcaa acgctgcttt cgcgacaagc tatgtttccg
    16561 cgatgcggag aacgagagag atgttcactt gtacatatca gcccagaaaa aactagccaa
    16621 tccgggctag atcagacacc ccggttcttt tcagaagcgg cgaacaccgc tcgcctacga
    16681 tttcttgccc tttttctggg ctttcttttc ccgggcagcg gccagaaggg cggcggcatc
    16741 ggcagggagt gcaaacgggc ggttcttgat ccgcttggta acccgcccac tgcgaataca
    16801 ctgcacgcaa acccgtaccc gccgtggggc accattctca tccgtgatac ggatccgctg
    16861 gagattcggt cggaactgac gcggcgagat accagtgatc tttgtaccca cacctccgag
    16921 gtacttggct ttaccccttg ttgtgacccg ctttccaatc gctggcgttt ttccgcagat
    16981 ttcgcacttt tgactcatcg ttatcagtcc cagcttattc aagggtggcg ctgatctcgc
    17041 gcctatctct tccttttgtc ctcttgtcct ctggcaaaca gacgatggca gggaaaacac
    17101 tcaatatact gaatccccct gcgatgacaa gtggagcggg ttggatcgag caccaaccgt
    17161 aggagctgag ggccgggccc ccgtgcccgc cctagaaaac cggacgctac gccaacgatt
    17221 gccaacccgc ggtccctccc gggattctgg gggtaaccac ggggggttgc ccctacggtt
    17281 agaatcgagt ctaagcccct aggttggcga tagacagctt ttccctcgtt cgcctggctg
    17341 cggaaaaaga ttcgtgggtt actttacctt gacgtggagc ctacgcggga aagcagggcc
    17401 ctccacccgg cgcgcggagc cggtaatcta ctgagtgttg ctctggaagt gttgcggaag
    17461 aatagctccc gctgtaaaca gcaaagaggt tacacaatgg catcgcccta taataacgca
    17521 ccataacccc accaaagggg gggcaagagt gctgttgtgc gtcacttatt cagcaaccct
    17581 gccacattgc tatggcgtta tgattatgtc aaattgatga tatcggcagg ttgtgcagca
    17641 tctcggagtt ggctcatctg tactgccgat ctggagcggt tccgggccgc ttcgtcgttt
    17701 cgtgatcagc acattctctt gggtatgcta tattccacta ggggttgaca cctgaactgg
    17761 tcgttttggc cccagcagct caaagcacgt tttttgagcg atggggatgc aggcaagggt
    17821 cggccagcgg ttggtcgcac cctatggata ggtcgtgttt gagggaattt tttgacgaaa
    17881 ggaatgagct gtgtcggact tcaatcttcc tgaacccggt gccgagcaac aagaggaaaa
    17941 gacggctcaa cagccgaccc cagaggctca ggcccagcaa cccgcgggac cgcctcgcgt
    18001 gaccgtgcag atcgacgata gcaaaatcgt cgccagctac gccaatttct gtcgcgttac
    18061 ggggacaccg gaggaggtca tcatcgactt tggcctcaac ccgcaaccga ttggcatccc
    18121 gtctcagccc atcgcggtga gccagcggat cgtgatgaac ttttacaccg ccaagcggat
    18181 gctccacgct ttacaactga ccatccagcg gcacgaggcc actttcggcg tgctggagac
    18241 agacatccaa aagcgtgtgc ggcctatgcc cccgcggcct aacgcgtgaa caaccagatg
    18301 ccaagagccg gcgatcttgt gtgggtcctg gacacagcgt cccattaggt cgtggcagaa
    18361 agaccaattc gcccgccgtt ttctgatggt gggcgtttta ttttcgtccc ggcttagggt
    18421 ctaccccgta gtcttccctc gcccgttcgg cgggagaggg gtcgggggtg agggtgcctc
    18481 gaattgctcg cggttttctc aatcgattgc gacgttcgct tgccctcacc ccaaccctct
    18541 cccggaggga gagggggact ttttgggata ggctcttatc gaaggaccgc tcgggtaaaa
    18601 cgaacgaaac gaccatcgtc cttgctgatc ggcagatcgg accgcaagga cctacttact
    18661 cccagagcca gaatgttccg gtcctttgcg tgcgctagca cggagtaaca gcgttcgcgc
    18721 tttccaaaaa actcgaaaat agcaagctgc gcaccagttt ggcgtttgcc ccatctggcc
    18781 ttcccgggat gggcgacctc ccggacgaac acccgtgtct tgtccgccat tcgattgcta
    18841 aaatggcgtt gactcctcgg ctgtctttgg cctgggtgtt gtccgatagc gactaccagg
    18901 aggagggctt ttctctatga ctgcgcggca gatgcgccga cacgccaatg aagaccaagg
    18961 cggttcagct ccggcaccgg ggccagacgc tttagcatct ggaagcccct cggtggcgca
    19021 agccggttca ccggagaatc aagaggagct tccgcccggc gttgtcgcca ccgctgagtg
    19081 gttagtctct ttttgtcacg aggccctttt gcaacagcaa acccccgagg ccctagccgc
    19141 gcgggcatat ctccagatgc agggcctagc ctggtcgggc atcgccgatt tacccattgg
    19201 cgttctgaac gatgcgagca aggcccgaga tatctaccag gatcgccaga tgggttctgt
    19261 tgaggacatc gaatattggc tcagtgatgc ccggttaagt cgtgtccttc ttgggccgat
    19321 ccgggatggt cgcgggaaac tggtgacgct ttgggcacga cccgtcgagc caactcaacg
    19381 gacccttttg taccgtcgcc cgtggcagga acgcgtcgcc gtttatgggg ccgagtgtct
    19441 ttctacagtt gaccctgttc ccgtttacgt tgtggagaag atccttgatg ccttggtttt
    19501 gcgatcccac aagatccagc cggcggtggc gtttgggcgg cgattcgacc aggtgcctgc
    19561 tcaaagctgg gcgtggctgg ggcggaacgt ggcaggcccg atcgtcttga tcccggtggg
    19621 ttcccacatc ccgagcacca tatttcggcg cgtgcggatc caggtggagc ggttgattga
    19681 tccgcccgag atttgggtgc tgccccccaa gcggatgttt gctccgcttg gtcggatggc
    19741 tgcggtgctc aaatccccgg agtttcagga gtttatccgc gatcgcgcgg tgccgctgct
    19801 agggcgaaag cggacgattc agttgaccca gggcgtggcc gcgatgcacc ggcagtccgc
    19861 cagcacggga ccggctcgtt acctgggcac gacgcaggag ggaccgaaga tcgacccgaa
    19921 ccagcggccg aaggaccttc gccctggcag cccggattgg ctggcgtttg attgaacggc
    19981 ctaagcggta gggtacacga attggccacg cttgatcgga agtggcctgc tctctcgaag
    20041 atgtgagaaa ctgtgccgtg cggacgtatg aaccttaaag gggggagaag cgataatcag
    20101 ataaataggg aataaaaccg acagggggag aggattctgc cgggccgcac tcggcaagat
    20161 gacgcaggag gaaatcggca gaagataggc ggaatggcaa aaggacagaa agtccgccgg
    20221 tcgcaacgcc caaggatggc tgccgtttcc ggtgggtgac gatgttctcg cgtttttccg
    20281 ctggacattg gcgattcttt gggtagaatt aaacgacggg ccccatgcga acccagcctc
    20341 ggtcgaagag gcgcgcgggt aacgaattgc taaaagcatc ccgccttaaa gatcactttg
    20401 gggcacggcc gttcctgtcg ttaccgttgg atcgtgtgcg ttttggcgat tcgggaacat
    20461 ttgggaggct ttgccccgct catccgcggt ctgatgtctc cgtcctacgt tgacgatgat
    20521 ggtgcatcgg cggagttgcc gtttgaaggt ggtggggaag gtttgagtag ccaatactct
    20581 gtttggtgtc ctgaacgaaa acgcacgatc cttctcgtaa ctatgtggcg ccattgggtg
    20641 gaaaaacgac ccccctttgg tcgtccgggg cggccccagt cgctcaaccg gtcgtagtgg
    20701 tgatgggagt aaacctaact gtttcgggcg tgtcggtgat tttctagagg agtatggcct
    20761 tgtacgaagc cgatcatctg tttgacgaac tcgaattgcg atcgatggag gacgaggagg
    20821 aactggagtt ccagggaagg ggcttgctgg agatggccga atcctacgcc gatgatgaat
    20881 tggccgagga cgaggaggcc atcagcgaag aggcgtatca agccgccgcc aatctggagg
    20941 agtctgagga agatacgatc ctcacggccg acgaggagga gtcctggtcc gatgatcccg
    21001 tccgcatgta cctgacgcaa atgggcgaaa tccccttgct ctcgcgtcag gaggagatcg
    21061 cactggcgaa gaagatcgaa atcacgcgag ccaagtttcg tcgcaagctg atggaatgcg
    21121 actacgtcct tcagcaggcc gtgcggactc tggctcgggt ggatgaaggg aaattgccct
    21181 tcgatcggac cgttcaggtg tcggtcactg atcatttgga aaaggagcaa atcctcggcc
    21241 gtttgccgca caacctgagg acgcttcaag ccattcttaa gcgaaaccgg caggactatc
    21301 tcacggcctt gagcaagtca aagccgatgg aagaacgccg ggcggcgtgg cggcgtctgg
    21361 gacgttcccg acgtcgggcc gggcgtttgg cagaagagtt gggcctccgc acccagcgcc
    21421 ttgagccgat gatcaaggcc ttggaggagt tcagccgtcg ggtcgatgag ctgaaggccc
    21481 gtatccaaga gcacgccaac gctggacgac cactcgaaga acgcaaaccg tggatcaagg
    21541 agtatcgtga catcctccgg ctgacgcagg agaccccgac gagtctgcgg aatcgcgtca
    21601 actacgtcaa gaaaatctac gcagagtacc aggaagcgaa gcgggccttg tccgagggaa
    21661 acctccgcct ggtggtttcc atcgccaaga agtaccgcaa tcgcggcctg agcttcctcg
    21721 atttgattca ggaaggcaac gctggtctca tgcgggcggt ggataagttc gaatatcgcc
    21781 gcggattcaa gttctgcacc tatgccacgt ggtggattcg tcaggcgatc acgcgggcgg
    21841 tggccgacca aagccggacc atccgcatcc ccgtccacat ggccgagacc atgaccaaag
    21901 tccgcaacgc ttcgcgacgg ctctatcaag agaagggtca tgaacccacc ttggaagaga
    21961 tggccgctgc cgccgaggcc aagaccgagg aaacccggcg tgttctcacc atgaaccgct
    22021 atccgatcag tctcgatcgc ccggtgggaa atagcgagga cagccatttc ggggaccttc
    22081 tgccggacac ctctgcggaa agcccgctgg atggtgccac ccacgatatg ctccgggaaa
    22141 agatcaataa ggtgctgcgg accctcagct accgtgagcg ggagatcatc aagttgcgct
    22201 atgggctggg cgatggctac tgctacacct tggaagaagt gggccatatc ttcaaggtga
    22261 cccgtgagcg tatccgccaa attgaggcca aggctgtgcg aaagctccag caaccaagcc
    22321 gcagccagga attggtcgga ttccttgatt gannnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nntgtattaa aaacggcaat cagacggcag ccgtccgatc aaaattgtcg
    22501 gttatggata agcaaggtga gcgcgtggct acggtgagga tgagtgacga aaaaagcgct
    22561 tgcgggcgag tttgcgattg aagcagtctg gctgacgggc tagaattggc ttcagaattg
    22621 gaatggtgcg gcgaacaaag tccccgcggc cgcggttgac gtgcggctgc ttgttgaaga
    22681 attaaggtca cggatggtca aacactcctg gcttcttttt ctttggcccg ggatggcggc
    22741 cctgtgggtt cgtcccacgc ttacggcctg gctgggaagc gcggcggctg ccgtggtctt
    22801 ccacctggcc ctggcggcaa ccatcctttg gccggagtgg ctttcgccgc aagagcgaat
    22861 gcttgtgtgg atcgccctag gggtggcctg gctcagcggg gtggcctggt cgtatcagtt
    22921 cctccgccga gaagaggcac gacagcaccg tcggcctgcc gaggaccgtt tcccggcagc
    22981 tacggaagcc tatctccggg gaaatctcgc ggcggccgag tccctggtgc gggaaattct
    23041 gcgggaatgt cctcgtgatg tcgaggcagc tttacttctg gccacaatat ggcgacgtcg
    23101 ggaacaattc gatgccgccg agaaactgct gcgaaacctc gcccggctgg acttggccat
    23161 cccctggctt tgggacatcg aacgagaact tcttcgaatc caaaaatctc gtgaagaaaa
    23221 ggccgatgag ccctcgccac aacccgagga ggagcaaact cctgtagaag aaccggtggc
    23281 cggggaaccg cctccttctt ctcaaaagac cgtccccttg cccgaacgag tgccggcagg
    23341 gaggacggcc cagccggcca ccaaggccgc ctgaggtgcg tcgcttttag agccaatccc
    23401 aaaaaagtcg ccctctccct ccgggagagg gttggggtga gggcaagcga acgtcagaat
    23461 tgtattgaga ttgagaaaat tggcagcgat tcgagccact ctcaacccga ctcctctccc
    23521 gctgaacggg agaggggaga ttttgagaca cgcttttagc caaaccagtc tggtcggtgc
    23581 ctaacgtgat cgggggtttt gggggcatat gagaaatctc tgtgcgaata gcacaacaga
    23641 tgcgagaaat cagatataat tcggtggagc cccgaagact tggccgcggg gcgaacccgg
    23701 tgtgccaagc ttcggaggat gggaactaag cccaacaagc cctgcgctgt catcatgact
    23761 acgcagaact gccacaatga cagtttgcca aaatggaggg atggagtaaa ctttggcagg
    23821 caagcaggca tcggcctgca cataatcagc ccagtgtgcg tagaaattta cgaaggtttt
    23881 ggttgtagaa aatcacgtag tgcggttttg ggcgggttat cccgggccaa aacaagtttt
    23941 gatggtacgg gagttgcaac aatgttctgg gtgggtcagc ccacctgaca agttgacagc
    24001 aacttggtgc aggggtgtcc tgcccaagtg cgggaagggg atttaaaaga aacacaagcg
    24061 aggttgtcgt cccagtggta agttggcgaa gccttcgcat catttgagtt aaggagtttt
    24121 gagagattat ggcgttcgaa agattcaccg atcgtgcccg aaaggtcatg cagttggcca
    24181 accaggaggc gcagcgcttc aaccacgaat acgtggggac ggagcacatc ttgctcggtc
    24241 tcatcaagga aggcagtggt gtcgctgcga acgtcctgcg caatctcgac gtggatctga
    24301 aaaagatccg ccttgaggtg gaaaaacttg tgcaaacagg accggatatg gtcaccatgg
    24361 gccgtctgcc gctgacgccg cgggcccgca aggtgatcga gtacgccacg gaagaggcgc
    24421 gaaacctcaa tcacaactac atcggcacgg aacacctcct cttagggttg ctccgcgagc
    24481 aagaaggtgt cgccgcccag gtcctcatga atctcgggtt gaagttggaa gaggtccggg
    24541 aagaggtcct caacctcctc ggtcacggca gtgaggccgc cgagactccc gatcgcaacg
    24601 cagggttggg tcaggatgaa ggaacgggcg aaccgtcggc gcgggcaagt cgttcccgaa
    24661 cacccgcctt ggatagcttc ggtcgcgact tgaccgaact ggcacgccaa ggcaagctcg
    24721 accccgtcat tgggcgggaa aaggaaattg agcgcacaat gcagattctc tgccggcgga
    24781 cgaagaataa tcctgtcctg ctgggtgagg cgggcgtcgg taagacggcc atcgtcgagg
    24841 gctttgccca acggatcgtg aaaggtgacg tccccgaaat cctgctcgat aagcgggtcg
    24901 tggtcttgga cctggccatg atggttgccg ggacaaagta ccgcggtcaa ttcgaggagc
    24961 ggatcaaggc cgtcatgaat gaagtgcgtc gcgctgggaa cgtgatcctc ttcatcgacg
    25021 agttgcacac gctggtcggt gccggtggcg cggaaggcgc cattgacgcc tcgaacgttc
    25081 tcaagccggc cttggcccgt ggtgaaatcc agtgcatcgg tgccacgacg ctcgacgaat
    25141 accgcaaata catcgagaag gacagcgccc tcgatcggcg gttccaggtg gtcatggtgg
    25201 agccgaacac caaagaggag acaatcgcca ttctcaaggg cttgcgtgac cggtacgaaa
    25261 agcatcacca cgtgcagatc acggacgatg ccattacgac ggcggtggag ctttccagcc
    25321 gctacatcac tggccggtgt ctgcccgaca aggccattga cgtgatcgac gaggccggtg
    25381 cgagggtgca cctgaaatcc ctcacccgac cgcccgacct caaagaaatc gatgaggaaa
    25441 tcgagcggct gaataaggaa aaagaggagg ccgtggccaa tcaagacttc gaaaaggccg
    25501 ccaagctccg cgatcaggcc gataagctca aaaagaagaa agccaacatg atgcatgagt
    25561 ggcgggagcg ctcggcctcc gtggacggca ttgtggatgc cgaagtcgtg gccgaggtgg
    25621 tctcgaagat gacgggcatc cccctcacgc ggatgacgac ggaggattcc atccgcctgc
    25681 ttaagatgga ggaggagctg cacaagcgcg tcatcagcca ggatgaggcc atcaagtcca
    25741 tttgcaaggc ggtccgccgt agccgaagcg gtctgaaaga ccccaagcgg cccgtcgggt
    25801 gcttcatctt tgccgggcct acgggcgtgg ggaagacctt gctggccaag gccctcgccg
    25861 agttcatgtt cggcgatgag gacgccctta tccaaatcga catgagcgag tacatggaga
    25921 agcacaacgt cagccgcctc atcggggccc cgcctggcta tgtgggctac gaagaaggcg
    25981 gacaactcac ggagaagatc cggcggcgtc cgtactcggt gatcctcctc gatgagatcg
    26041 agaaggccca tccggacgtc ttcaatatgc tgctccaggt catggaagaa ggccgcctga
    26101 ccgacagctt cggtcgcaat gtggacttcc gcaacacgat cctcatcatg acgaccaatg
    26161 cgggcgccga ggccatcaag aacgaggccg ccttcggctt ccagaagccg gacgatgaca
    26221 ccagctacga aaacatgaag cgacgggtca tcgagcagat cgaaaaggtc ttccggccag
    26281 aattccttaa ccgcgtcgat gacatcatcg tcttccgtca ccttacgcag aaagacctct
    26341 tcgacgtggt cgaactggag ctcggaaagg tccgctcacg gttggcggag cgaggtttgt
    26401 cgctggtcct cactgaagag gccaagaagc taatcatcaa gcgcggtacg aaccttgatt
    26461 tcggtgcacg tccgctgcgt cgggcaatcg agaactacat cgaggacccg cttgccgagg
    26521 aactcctcaa aggtgagttc gagggcaaag acactatcgt cgtcaaggtg aaggacgtcg
    26581 gcgggaagaa acaactggtc ttcgagggtg tcgtcggcca gccggacttg tcgtcggaag
    26641 gtgagacatc cggtgatcag cagccggaag tcgccggcgt ctccggcggc gaagggtcac
    26701 cctcgccgtc ccaggaatga cggaaagacc gccgtcc
//
LOCUS       DAOSUT010000045        26359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1213_length_26359_cov_9.588085, whole genome shotgun sequence.
ACCESSION   DAOSUT010000045 DAOSUT010000000
VERSION     DAOSUT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 26359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26359
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1213_length_26359_cov_9.588085"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="PLD05_05810"
     CDS             <1..1741
                     /locus_tag="PLD05_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOP76986.1"
                     /translation="YQQRLIQSNAVDFDDLLIHLAILLRQHPEIRKRLDDRYRFVMVD
                     EYQDTNLVQYAIARAISVDHPNLAVTGDPDQSIYGWRGANLNNILEFEKDFPNVRVVR
                     LEQNYRSTKRILAVAARLIAHNVKRKEKDLFTENAEGRPVRLMTYANQEDEARDIARQ
                     IAQAVATGQRRYRDFALFYRINALSRSFEIAFREFGIPYQIVNGVEFFQRKEIKDIIA
                     YLQLINNPRDDVAFLRIINTPPRSIGETSVKRLRQFAAEQRLSLLEAARLADRIGEFS
                     TRTVGAFRGFVNMMDRLAAAADGPVEELLGLVMTESGYRAMLEKSSREEDLERLANIE
                     ELLTAARQFDERHQEEANLERFLEETALVSDTDKMDDRSDRVTLMTLHASKGLEFPVV
                     YLVAVEQGILPHERSRNNDDELEEERRLMFVGITRAKEELQISLAEYRDFRGQRQLTI
                     PSQFLMELPRSELEIVQSPRAVGRVSTFETATGSWARVVSSRFENGNRSQSGSSTSAI
                     PPRTPSTSASRRGPLTPDDFVLDMRVVHPRYGVGRIVALSGSGESRKATVDFGGSIGR
                     MKFVLSQSPLKPK"
     gene            1764..2582
                     /locus_tag="PLD05_05815"
     CDS             1764..2582
                     /locus_tag="PLD05_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HOP76987.1"
                     /translation="MMEENHSIEQPTHISQEAERRWRPLPPIERRILGVLVEKAKTTP
                     SAYPMTLNAIVTGCNQKSNRDPVMELNEEVVDRALENLRQMGAVGRVQGLGRVDKYRH
                     YLYEWLNVEKEELSIMAELLLRGPQTEGELRAHVSRMDPIPDLPTLRQHLDRLTKKGL
                     IVHLTPPGRGHTVAHNLYPEPELARLKQRYAALAVTDTAFADSEPPSSSLSAPAATAT
                     MARAGGDTVGRSAETSPPPQAHEIDALRSEIAALKETLTRVVGDVERIKRELGL"
     gene            2859..3995
                     /locus_tag="PLD05_05820"
     CDS             2859..3995
                     /locus_tag="PLD05_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HOP76988.1"
                     /translation="MKATSVLRVRGLPMACVWAACAAATWAVFVTTLTVAEEMASREQ
                     NVPNLAQQVPYPRITLSVCYEVDPTWPQRPAELVWGQTPSVFVDNQDRVWVYTRAVPP
                     VHVYDAATGKLLFTWGEQVFGKYLEKMLAHGIKIDHRGHVWLCDVGNHAVFELDQTGK
                     ILRILGTPGEPGCDQSHFYKPTDMAITPQGHVFVTDGYGNARVVHFDDQGRYVGEWGT
                     LGVKPGEFNLPHAVVVDSKGRVYIADRNNARVQVFDQSGKLLDAWQDLVVPWGFWITA
                     DDQIWVCGCSPMPWRETDEVLSCPPKDQLFMRFDPTGRVRQLWTVPKGIDGQEQPGEL
                     NWVHGIALDSQGNIYACDIIGKRVQKFVRKSPGWSVPFVPKPKE"
     gene            complement(4115..5278)
                     /locus_tag="PLD05_05825"
     CDS             complement(4115..5278)
                     /locus_tag="PLD05_05825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOP76989.1"
                     /translation="MPWLLTVIPCSLLISWLGGFVLRRYGPRWGLVDRPGARKVHDRP
                     MPTSGGIAIWLGLIVPLAVLQGMLWLTFDARPAPQRAPEVIAGLVARDYLPEEIVRHL
                     PGVWAQSGRLWTVLGGATLVMLLGLADDRWKLPWWLRLVAEFILATATVAAGFRLTVF
                     IDIPILTWILSALWIVALINAFNMIDNMDGLSAGVAAIACTILAAVMLLTPKELGGEP
                     QLFVAGFFLLVLGALLGFLWHNWPPARLFMGDAGSYLIGYLLAVISMAATFAGEHTPR
                     HAVLAPLCALAVPIYDLVTVVTIRLRAGASPFVGDTRHFSHRLVELGMTKAQAVLTIY
                     LMAATCGLGALLLHQVDTFGAVVILLLVGCIVSLIAILETTGRRAQRRRNNKT"
     gene            complement(5263..5823)
                     /locus_tag="PLD05_05830"
     CDS             complement(5263..5823)
                     /locus_tag="PLD05_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOP76990.1"
                     /translation="MGVPTAPDPVLLLLAVFSRYDEAIEWARQRIVEEWGPIALTSPP
                     FPFTHTDYYTPTMGPGLRKVFFAPADLIDPGRLPAIKIQTNAWESEYAEMKRFPEPRP
                     VNIDPGYLTLGKLVLASTKDFAHRIYLGLGIYGEVTLFYRHHRWEHHEWTFADYRQPE
                     YHEFFSRCREYLHERLREVTAPCRGS"
     gene            6164..7345
                     /locus_tag="PLD05_05835"
     CDS             6164..7345
                     /locus_tag="PLD05_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOP76991.1"
                     /translation="MIRQELIILCLALVVPATQRTWAIELSGVFVPKFTVEKAPELEK
                     WFDASDKWGGADGVFSIPLDEHRLLFTFGDTFTTRQRRKMVNNSIAIMQLEGRTPVDV
                     AFYFGQTTDGEIRSFAVPEDHRGLFWLAAGFRDGDRLHLFAAHIELTPMGGPFGFRQI
                     GEALLTVNNPDDPPPQWHIQQQALPYFRSDDQGTAVMGSAVCVPAEANSQYVYLYGFE
                     EEKTPLFPIKHAIVARVAKGRADHINEWEFYCGPSQLPPNAQPQDFIIRAENWTRDFS
                     RATRLAREVASEYSVSYVPCLQAYVMVYTRLGLSQEIMMRRAPTPVGPWSEALRLYRV
                     PDLPEDQKAFYYAAKAHPALAADDELVISYVANSFDFGRLVRTPEIYRPWFIRVRFIP
                     E"
     gene            complement(7520..8320)
                     /gene="hisN"
                     /locus_tag="PLD05_05840"
     CDS             complement(7520..8320)
                     /gene="hisN"
                     /locus_tag="PLD05_05840"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOP76992.1"
                     /translation="MGAASEIQGRLDFAVEAARAAGEITLRYFRRADLAVERKADASP
                     VTLADREAEAYLREAIHKRFPQDGIVGEEWGTDEGSSGFRWIIDPIDGTKSFIHGVPL
                     YATLIGVEWEGRAVIGVDRIPATGEVIFAAKGRGAWYQIGEDPPQPARVSRCENLAEA
                     AWVTSEVLNFDKINRREAFHRLQSATRLFRTWGDGYGYLLVGTGRIDFMIDPLMEIWD
                     AAAIQPIIEEAGGIFTDWRGQPVFTSGNGIGSNGLLHNQVLDVLRGTD"
     gene            complement(8341..>8550)
                     /locus_tag="PLD05_05845"
     CDS             complement(8341..>8550)
                     /locus_tag="PLD05_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76993.1"
                     /translation="HGQAQGPAPMMDGFPGRLTPVGATPCGCPNGPVIAATSWAGTGA
                     CPYNVFGYFCDNGGFGECKPIWATI"
     assembly_gap    8551..8650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9061..10023
                     /locus_tag="PLD05_05850"
     CDS             9061..10023
                     /locus_tag="PLD05_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOP76994.1"
                     /translation="MTLCRFTCPECKQSFTMELPDDTDLAKVTCPQCQSILTVKVPSE
                     PPPIPEVPTDQEPPSQLVVPPPIPPTLPPEREASSGQIPLPSDILTQGHRNSEMASSP
                     ETSVVPPALAPYAPPTAPVSVAAEHACPLCGRSDPKYFVRGKMLYGHKVCRKCYLSFL
                     NRRQFAFAFDIVAYFILLLGVNFLEEFTVGFVLSATQAELIEQQLEAILLAFNVLNYV
                     LYALWFLFKDGFNGTSLGKAIMGLFVINEATGKRISFGGSAKRNWIIVLLGLVPFAWL
                     IIAAIMGKGYRIGDRMANTKVVWKKYAHSPVFAPNRAVLSSPGL"
     gene            10174..11592
                     /locus_tag="PLD05_05855"
     CDS             10174..11592
                     /locus_tag="PLD05_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP76995.1"
                     /translation="MSTRWRRTLIFVGISLTLMGIAWAPLAYAQRVRFPTASLTQDPG
                     QSIYQQVPPAGGTGVSAPSAASGTTAPGYQSTITQPSGVPSYAPPAGVSGPPIQGYGT
                     TAPGVTGSGPYPSPWDPIPTAPPPTGTAGSAVGGINYPPGQVPYYAPAGPTVPPAPGA
                     VYQGTTPPPPTWDPYAVPGNPSPSVFPQGGIFPGVQGDSFYNTMIRFREATGFRFTWI
                     GGSDLGVNDLELYSTFAFPFLTTSGPPLRVTPGFAFHWWDGPVSSGLPESADMPSVTY
                     DAYLDAGWNPQVSPVLGGELNVRVGVYSDFKRVTSDSLRLQGAGFLTLALTPNWKLKG
                     GVIYLDRVRLKMLPAGGAIWVDNPQNPTMELALVFPSPRFAMRLPQQTTADVWWYVRG
                     DYGGGSWTIKRTSGANIGATEQVDYNDLRLATGLEFYRLRGLRGYAEVGLAFSRELVY
                     EAGWTDKYKPDAAVFLSGGLAF"
     gene            11823..13886
                     /locus_tag="PLD05_05860"
     CDS             11823..13886
                     /locus_tag="PLD05_05860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOP76996.1"
                     /translation="MSTEQPLDPQLIEQTRQQIRQLVAEVAELSSRTDLTPDQYCAEF
                     LPRVVSALAAFGGVFWTVEGPGRISLTYQMNLQQTRLAEDEEARQKHNRLLQRVLQTR
                     EAILVPPQSGSGENNGEANPTDFLLLLGPVGTELEVVGVVEIFQRPDAPPAAQKGYLR
                     FLMQMCDMASDYFKSLQLRSLSDRQSLWSRLEEFTRLIHQSLDPAETAYTLANEARRL
                     IECDRVTISVRRGRRQVIEAISGQDVVNKRSNVVRLLGDLATVVATARENVWFCGDTS
                     QLAPQIEDALQQYVDESHSKTVIVIPLGRPAPPPSDEAEADRPADPPPPVGTMIVEQI
                     EDSRITPQLRQRVEVVAQHGAVALANAVEHNEIFLMPLWKAIGRSKVITEARMLPKTA
                     AVGIVIVLLLLTLLFAPWPYKLHARGTLEPVLRRDVFAGIDGIVDEVLVVHGQHVNQG
                     DILARLRNSELKQQILEIEGQLAANQKQVATIRRELADLRMLSAQERNRLTGQLAELE
                     ARQISLAAQLAILREKEKELTVLSPISGDVVTWDVYNRLINRPVNRGQALMRIADTSG
                     EWELELHMSEDRMGEIALARQQVKAQDPSQDLTVEYVLATDPGRTRTARVKDVYVSAE
                     VLPEEGNVVMIKVAVNKDDFDATQLRPGATVSAKVHCGTRSMGYAWFRDLIAFIQTRV
                     LFYLW"
     gene            13933..14808
                     /locus_tag="PLD05_05865"
     CDS             13933..14808
                     /locus_tag="PLD05_05865"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOP76997.1"
                     /translation="MAWLWVAVMVLATETNANTITLANCPVSIIDEAQVPARVQGQLV
                     ELTVKEGDSVTKGDVLGRIDDAVLRKTLEVKQHQLAAAQKEADNDVNVRYAKWANLVA
                     DAEYQQAVDANNRAPGAVPQAEMRRLLLERGKTSLAIEQAAHDLEVAKLTVQVRQAEL
                     AEVEEQLEMYRIVAPLDGVVVKRFFHAGEWVKPGDTVFHIIRMDRLRIEGMVRADQYT
                     PGQLANARTIFTVKLPHLGEQRFEGQTVFVSPVVEVTGEFTVWAEVTNIRDPKNGQWL
                     LRPGLTGQLTIQLNP"
     gene            14912..17158
                     /locus_tag="PLD05_05870"
     CDS             14912..17158
                     /locus_tag="PLD05_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOP76998.1"
                     /translation="MVTLADSLLSSSARKLSIRKRADLVAQKQRYEGETYYVIKDPVG
                     LNYFRFQEEEFFILNQLDGQSSLDEIKARFEAEFPPQKITLEELQQFVGMLHRSGLVV
                     AVVPEQGRQLLKRRRERRKRELLSALGNILAIRFRGIDPERILTAMYPWVRWVFHPAF
                     LIACVVLWIAALLLIASEFRVFLNRLPTFHQFFTPTNALWLALTLAVTKIIHEFGHGL
                     SCKHFGGECHEMGIMLLVLTPCLYCNVSDSWMLPNRWHRAAIGAAGIFVELTLAALAT
                     FIWWFSEPGLINHLALNVMFICSVSTLVFNGNPLLRYDGYYVLADILEIPNLRQKATQ
                     LLSRTAAHWFLGIKPPDDPFLPQRNRALFVLYAIAASIYRWVVVLSILLFLNRFFKPY
                     RLEIIGQMIALGALYGLVVQPLYQVGKFFYIPGRWNQVKKPRLYMTLTGLGLVLAFIL
                     FVPLPYRVMCTCEIQAKDAAPVYVDVSGRLEEVLVRPGQAVRLGDSLARLSDYDLEIQ
                     IARLETLQRQYEARLAVLERRRSEDEAAANELPALREALQTVTQQLAEKHRDRDRLLL
                     RAPCDGVVLPPPWKTVTADKNDPLPDWSGTPLEPRNIGAFLREGELFCLIGQPQNMEA
                     ILVIDQADIEFVQPGQLVRLKLDELPWRTLRGTIEDVAKREVEAASPRLAGKAGGELA
                     TKTDPRTGVEKPISTVYQARVPLVNDENLLIPGLRGRAKIHVDRQHWLTIGQRVWRFV
                     TRTFNFRL"
     gene            complement(17362..18786)
                     /gene="hydG"
                     /locus_tag="PLD05_05875"
     CDS             complement(17362..18786)
                     /gene="hydG"
                     /locus_tag="PLD05_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOP76999.1"
                     /translation="MSHVTRSRRLSQSAVDFIDEPYLFSLLEQPAQAGEVREVIAKSL
                     SKEPLTVPETAKLLTIQQPDLWEEVFDAARRLKRDVYGNRIVLFAPLYIGSECTNDCV
                     YCAFRRSNPNVVRRTLSPDDIESQVCHLLDRGHKRLILVFGEHPRYSPEFIADCVRRV
                     YAVKRPRANIRRVNINAAPLDHEGFATVKAAGIGTYQIFQETYHHETYAKVHPRNTRK
                     GDYLWRLDALSRAMEAGCDDVGIGALFGLFDWRFEVLGLVSHAWHLQRHYNVGPHTIS
                     FPRLRRAAGVTLDETYRVSDEDFKRLIAVLRLSVPYTGLILTAREPAELRRAVMEFGV
                     SQIDAGSRIEVGGYTEVGDAQVLEREQFELGDLRPLDEVIRQLMTDGYIPSFCTACYR
                     LGRTGEHFMEYAIPGFIQRFCTPNALSTLMEYLVDYASRETYETGVRLIENELAKLPD
                     GPRKTALIERLRRIRETEERDLYF"
     assembly_gap    18917..19016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19044..20132)
                     /gene="hydE"
                     /locus_tag="PLD05_05880"
     CDS             complement(19044..20132)
                     /gene="hydE"
                     /locus_tag="PLD05_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOP77000.1"
                     /translation="MFGTTVKEPALTREELLTWLREEDPARLSQLWQWADRVRQEFVG
                     GEVHLRGLIEISNHCVRLCAYCGLRAPNTELVRYRMSGDEILACARQAAALGYGTVVL
                     QAGEDPGLTRDFVREIVRRIKETTGLAITLSLGERDEEELWAWRQAGADRYLLRFETS
                     QAELYQRYHPPKPGSTVDRLYLLKRLREMGYEVGSGVMIGLPGQTYAGLANDLWLFRE
                     LDLDMIGVGPFIVHPRTPLADPAFARILPDGEQVPASELMTYKVIALSRILCPRANIP
                     STTALATLNRKNGRELGLVRGANVVMPNLTPPQYRIRYEIYPNKACLRETPDECHACL
                     QMRVESLGRVVGRGPGTSPNYRYRMAKD"
     gene            20497..21804
                     /locus_tag="PLD05_05885"
     CDS             20497..21804
                     /locus_tag="PLD05_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOP77001.1"
                     /translation="MPRRNLQILVLAIIICLLCAYRSSRYTRVLTYALEQIQFKALEP
                     RSPQQLTEGALRGMVGVLDPYSSYISEEDLPKFEEELDRQFAGIGVEIFLDPQSQRLC
                     VASPLPNTPAQKAGIKPGDWIVAIDGQSTEGMTLEEAGKKMRGEPGTQVVLSVQTPGE
                     EKPHEVKLTREIIRAETILGDTRNPDGTWNYVLQSHPEIGYVRISTFGEETTAQLKKI
                     ILDLKKKNIRGLILDLRDNPGGRLEEAIAMCDLFIESGVIVTTRYRNGQIKRQYTASG
                     KPVCPDLPMSVLINHYSASASEIVAACLQDHGRATIVGERSFGKGTVQELIQLEAGLG
                     MLKLTTAAYWRPSGKQIHRSANAGEDQEWGVRPDPGKEVKLDRAEQDMLARYRALRDI
                     YLPPGSPKPAGFDSIPADFRDRQLEVAVESILKGQETSAKSAE"
     assembly_gap    21940..22039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22040..22942
                     /gene="dxr"
                     /locus_tag="PLD05_05890"
     CDS             <22040..22942
                     /gene="dxr"
                     /locus_tag="PLD05_05890"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOP77002.1"
                     /translation="AVLEAVSADDVDMVLSAFVGIAGLRATWAALEAGKTVALANKES
                     LVVAGSLITQLARKRKAQILPVDSEHSAIFQALHGGQEREVRRIILTASGGPFRSRKL
                     TELASVTVEEALAHPTWKMGKKITVDSATLMNKALEIIEARWLFGVEADQITVVIHPQ
                     SIVHSMVEFVDGSVIAQLSPPDMRLPIQYALYFPERRPGIARKIDWATAMDLRFEPPD
                     LERFPAIRLGLEAARIGGTAGAVLNAANEAAVECFLQGRLRFDQIVPVCAKVLEAHPF
                     DPSPDLDAIFGLDAWARKEVERWI"
     gene            22933..25143
                     /locus_tag="PLD05_05895"
     CDS             22933..25143
                     /locus_tag="PLD05_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOP77003.1"
                     /translation="MDINSLFFAFLGGAAWLYVLMVIVGLGLIIFVHELGHFVVAKLC
                     GVKCEKFYLGFDFFGLRLFRFRWGETEYGIGIIPLGGYVKMLGQEDNPARLREEFERA
                     KAAGQEGAKSSTGESLANMEEALYNPRSYLAKSVPQRMAIISAGVAMNLLFAFIAAVL
                     AYFIGVQEIPCGVGQLAPGEGAWRAGILPGDKILEINGQKTHRFQDLQQSISLARRGQ
                     VVTMRIERPTPNGLKVLDLELMPDRIRVVPTIGVTNPLLPILAEPPCYPGSAAAEVIT
                     SLPPGATVKAIHGEPVTGFADIARSWAKSWGQPVELMLEKKAASGTPLEPQKVVLQPT
                     PVRDLGLIFKVGRIVAVRAGSPAEVAGVLAGDELMSLTCPENVNWNGNFMRLPYALQK
                     ALRNRPEGIPLQLTVKRSNGSEKAGVALVAMRSATAADKNSSNEGTPLKLDVVAKTAE
                     FFADLNTGDSPFEIPQLGIAVEILPQIEAVEPHSPAAAAGLQPGDELISVTAIPPNLD
                     PESKIGELARHFSMTKFTVRFDQPRGEEKGTWPFFFRELQRMPTGTEVDLVVRRPQPD
                     PAQEYQEVKVHLVPRVADDWFLAERDLLFEAMTFDLRAAGLGEAITMGATETWTSVTL
                     IYRVLNSLGRGDVSPRALVGPIGLVQAAYRVASQGLGRFLLFLTLLSANLAVINFLPI
                     PVLDGGHMAFLIYEGIRGKPPSETVYVGLSYLGLAIILGIMLWVFGLDLGFISR"
     gene            25197..26270
                     /gene="folK"
                     /locus_tag="PLD05_05900"
     CDS             25197..26270
                     /gene="folK"
                     /locus_tag="PLD05_05900"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOP77004.1"
                     /translation="MTDLASSAPRLVAIGMGSNLGDSWTILRQACKALSELPCTQLRA
                     TSRIRLTQPIRVSDQGSPFFNAVALLETALPPRELLDGIMAIEQRFGRERNRHWASRT
                     LDLDILLDEAGPFYDQRLLIPHPRLGSRLFFLACLEELLPDWRHPWCGLTVRQMVEIL
                     STRPPYCVLIWETFLPTHEASSLTTHSGEIQAGEADWAALWQACVAANKVFQAGCPHL
                     EDSGGSVSAGVHLTHPPESCIFNAEEVQHRLAMGQSLAEIVSFAWLEHRKQDHNGRDT
                     PLPRVMILGPRAKDLLWPHEANASDRPLVSPSFELANLSWLRDQVTPVVFSEADDPRT
                     WAEHIIAAIDASAEVGHVLGDLG"
BASE COUNT         5529 a         7584 c         7497 g         5449 t
ORIGIN      
        1 ttatcaacag cggctcatcc agtccaatgc cgtggacttt gacgacctgt tgatacattt
       61 ggccatcctc ctccggcagc atccggaaat ccgtaagcgt ctcgacgatc gctaccggtt
      121 tgtgatggtc gatgagtacc aggacacaaa tctggtgcaa tatgccattg cgcgggccat
      181 ctccgtcgat catcctaacc tagccgtgac gggcgatccc gaccaatcga tttacggctg
      241 gcgcggtgcc aatctgaaca acattctcga attcgagaag gatttcccca acgtgcgcgt
      301 ggtgcggctc gagcagaact atcgcagcac aaagcggatc ttggcagtcg ccgcacggct
      361 tatcgctcac aacgttaaac gcaaggaaaa ggaccttttc accgaaaatg cggaaggtcg
      421 ccccgtccgc ctaatgacct acgccaatca ggaggatgaa gcccgcgata tcgcccgaca
      481 gattgcccag gctgtggcaa caggtcagcg ccgttaccgg gattttgcgc tgttttaccg
      541 gatcaatgcc ctgtcccgga gctttgaaat tgcctttcga gaattcggta tcccctatca
      601 gattgtcaac ggtgtggaat tcttccagcg gaaagagatc aaggacatca ttgcctatct
      661 ccaattgatt aataatccgc gggatgacgt ggcctttttg cggatcatca acacaccgcc
      721 gcggtcgatc ggtgagacga gcgtcaagcg gctacgacaa tttgccgccg agcagcggtt
      781 atccttgcta gaggccgctc gcctggcaga tcggatcggc gagttcagca cccggaccgt
      841 gggggccttt cgcggctttg tcaatatgat ggatcgtctt gcagcagccg ccgatggccc
      901 agtggaagaa ttgctcggtc tggtcatgac ggagtcgggc taccgcgcga tgttggagaa
      961 atcctcacgc gaggaagacc ttgagcgcct ggccaacatt gaggaactcc tcaccgccgc
     1021 tcgccagttt gacgaacgcc accaagagga ggccaatttg gaacgctttc tcgaagaaac
     1081 ggccctggtc agcgacaccg ataaaatgga tgaccgatcc gaccgcgtga cgctgatgac
     1141 cctgcatgcc tccaaaggac tggagtttcc cgtggtctat ctcgtcgccg tggaacaggg
     1201 aattctgccg cacgaacgca gccgcaataa cgatgacgaa ctggaagaag aacgacgact
     1261 catgttcgtg ggaatcacgc gggccaagga agagttgcaa atcagcctgg ccgagtaccg
     1321 cgatttccgt ggccaacggc agttgaccat tcccagccaa ttcctcatgg aactgccgcg
     1381 atccgaattg gagattgtac agtcgccccg ggcggtgggg cgagtctcga cttttgagac
     1441 ggcaaccggc tcgtgggcac gcgtcgtctc ctcacggttt gagaatggca accggtcgca
     1501 aagtggttcg tcaacgtcgg ccatcccgcc gcgaacccca tcgacttcgg cttcacgccg
     1561 gggaccactc acgccggatg acttcgtact cgacatgcgt gttgtgcatc cccggtacgg
     1621 cgtcggccga attgtggccc tgagtggttc cggggaatcg cggaaggcca ccgtcgattt
     1681 tggcggtagc attgggcgga tgaaatttgt cctctcgcaa agtcccttga aacccaagtg
     1741 attgtgagaa aaggatatcg ctgatgatgg aggaaaacca tagcatcgag caaccgactc
     1801 acatatcgca agaggcggaa cggcggtggc gccccctgcc tcccatcgaa cgccgtatcc
     1861 tcggagtgct cgtcgaaaag gccaagacga ccccgtcggc ttatcccatg acactcaatg
     1921 ccatcgtgac aggctgcaat caaaaaagta atcgtgatcc tgttatggag ttgaacgagg
     1981 aggttgtcga tcgtgccctg gaaaacctcc ggcaaatggg ggctgtcggc cgggtccaag
     2041 gcctgggtcg tgtcgataag tatcgccatt atctttacga gtggctcaat gtggaaaaag
     2101 aagaactttc catcatggcc gagctccttc taagaggtcc acaaacggaa ggggaactgc
     2161 gtgcccatgt gtcgcggatg gaccccattc ctgatttacc caccttgaga cagcacctgg
     2221 atcgcctgac gaaaaagggg ctcattgtgc atctgacacc gcccgggcga ggtcacaccg
     2281 tagcccacaa tctctatccg gagcccgagt tggctcggct gaagcagcgg tacgcagcac
     2341 tggccgtgac ggacactgcc tttgccgatt ctgagccgcc gtcatcttcg ttgtcggctc
     2401 cggccgccac ggcgacgatg gcgcgagcag gtggcgacac agtgggccgg tccgcggaaa
     2461 cgtctccgcc cccgcaggcg catgagatcg atgccctccg gagtgagatc gcagccctca
     2521 aggagacgtt aacccgtgtg gtgggagacg tagagcgaat caaacgcgaa ttggggctgt
     2581 aagccttgcg caaacccgcc ggcacctatg ggcgacacca ggtttgcctc tgcgacccgt
     2641 gtttcctctt tccatttagg ggccatgtcc gtgtgagcgg aataattcgc cgggtcacac
     2701 accgttctgg cggggaacga ctttgggtca ccaatgggga tgaccgccac gggggcgcgt
     2761 gaccttttct ctgctgcgct ttactttccc tcattgcctc gacacaatag tgggctgaga
     2821 tttcccgcgt gttcaaaatc ctatcaggag tgacgctcat gaaagcaaca tctgtcttga
     2881 gagtgcgagg gttaccgatg gcatgcgtgt gggcggcttg tgccgcggca acttgggcag
     2941 tatttgtcac gactcttacg gtcgccgagg agatggcatc acgggaacaa aacgttccca
     3001 accttgccca gcaggtgccc tatccacgga tcaccctttc cgtctgttac gaggtggatc
     3061 caacctggcc gcaacgtccg gccgaacttg tttggggcca gacgccttcc gtttttgtgg
     3121 acaaccagga ccgtgtttgg gtttataccc gcgccgtgcc gccggtccat gtgtatgacg
     3181 ccgctacggg aaagcttctc tttacctggg gagagcaggt cttcggcaag tatttggaaa
     3241 agatgctggc ccatggcatc aaaatcgacc accgcggcca tgtgtggctt tgtgatgtgg
     3301 gcaatcacgc cgtttttgaa ttggatcaaa ccggcaagat tcttcgcatc ttggggacgc
     3361 ccggcgaacc cggctgcgat caatcccact tctacaagcc caccgacatg gccatcacgc
     3421 cccaaggaca tgtctttgtg acggacggtt acggcaatgc ccgcgtggtc cacttcgatg
     3481 atcagggacg ctatgtcggc gaatggggaa cgttaggcgt taaaccgggc gagttcaatc
     3541 tgccgcacgc cgtcgtggtg gattccaagg ggcgtgttta tattgccgat cggaataacg
     3601 cgcgggtaca ggtttttgac cagtctggaa aactccttga tgcgtggcaa gacctcgtgg
     3661 ttccctgggg attttggatc accgccgacg atcaaatctg ggtctgcggt tgttcgccta
     3721 tgccgtggcg ggaaaccgac gaggttttaa gctgtccgcc caaggaccaa cttttcatgc
     3781 ggtttgatcc cacgggtcgg gtgcggcaac tttggaccgt gccgaaggga attgatgggc
     3841 aggaacagcc cggcgaactg aattgggtcc acggcatcgc cctagattcg caagggaaca
     3901 tttacgcttg cgacattatt ggcaaacggg tccagaagtt tgtccgcaaa agtcccggct
     3961 ggtcggtacc cttcgtgccc aagccgaagg aataatccac cggcggacaa tcgtcagcgc
     4021 tgcgggtccg accgcgaaag acgggaacta tccggtcggt tgggagcgat tgtggcaacg
     4081 cgcttgttgg ccatagtggc tcgcaggatg gccgttatgt cttattgttg cgtcgccgct
     4141 gggcgcgtcg tcctgtggtc tccaggatgg caatcaaact gacgatgcag cccaccagga
     4201 gcaagatcac cacggcccca aaggtatcca cctgatggag gaggagtgca cccagcccgc
     4261 acgtagccgc cattaggtag atggtcaaca cggcctgtgc cttggtcata cccagctcga
     4321 caagccggtg cgagaagtgc cgcgtgtcac cgacaaaagg gctggcgccg gcccgcaggc
     4381 ggatggtcac caccgtgacg aggtcgtaga tgggcacggc cagggcacag aggggcgcca
     4441 acacggcgtg gcgtggtgtg tgttcgccgg caaaggttgc cgccatcgat atgacggcca
     4501 ggagataacc gatcaggtaa ctgccggcat cgcccatgaa aagtcgcgca gggggccaat
     4561 tgtgccacaa aaagcccagc aaggccccca agaccaggag aaagaaacct gccacgaaca
     4621 actgtggctc gccgcctaac tctttgggcg tcagcaacat cacggcggcg aggatcgtgc
     4681 aggcaatggc ggccacgccg gccgaaagcc catccatgtt gtcgatcata ttaaaggcgt
     4741 tgatgagggc cacgatccac agggcactaa gtatccaagt caaaatgggg atatcaatga
     4801 acacggtgag gcgaaagccg gccgccactg tggccgttgc caggatgaac tcggctacga
     4861 gccgcagcca ccaggggagt ttccaacggt cgtcagccaa tcccaggagc ataaccagcg
     4921 tggcgccgcc caagaccgtc caaagccgac cgctttgtgc ccaaacgccg ggtaaatggc
     4981 ggacaatctc ctcgggcaag taatcgcgcg cgaccagtcc ggctatgacc tcaggtgccc
     5041 gctgcggggc cgggcgggcg tcgaaggtaa gccaaagcat tccctgcaac acggccaacg
     5101 gcacgatgag ccccaaccag atggcaattc cacccgaggt gggcatgggc cgatcgtgga
     5161 ccttccgcgc cccaggccga tcgacaagcc cccacctggg accgtaacgt cgcagcacga
     5221 acccgcccag ccaacttatg aggagggaac agggaatgac agtcaggagc cacggcacgg
     5281 cgcggtcacc tcccgcaaac gttcatgaag atactcccgg cagcgggaga agaactcgtg
     5341 gtactctggc tgccggtaat cggcaaacgt ccattcgtgg tgttcccagc ggtgatggcg
     5401 ataaaacagg gtgacctcgc cataaatgcc caggccaagg taaatccgat gggcaaagtc
     5461 cttggtcgaa gccaggacca atttgcccaa cgtcaaatag ccggggtcga tattcaccgg
     5521 ccgcggttcc ggaaatcgct tcatctcggc gtattcgctt tcccatgcat tggtttgaat
     5581 cttgatcgcc ggcaaccggc cggggtcgat caaatccgcc ggcgcgaaaa agaccttccg
     5641 gagaccaggc cccatcgtcg gcgtgtaata atctgtgtga gtgaagggga agggcggact
     5701 cgtgagggcg atcggtcccc attcttcgac aatccgctgg cgggcccatt cgatggcttc
     5761 gtcataccga ctgaagacgg ccaggagaag caacacggga tcaggggccg ttggaacacc
     5821 catttttaca tctcctcaag ggaaggaaca acgtactcaa gggatttccc agcgatgtca
     5881 tttccggaga cgatgactct tgcttgattc taccatcatc cctcacacta atgggaaatc
     5941 ggtctcggcc attcagagtc tatcccaaaa agtccccctc tccctctggg agagggttag
     6001 ggtgagggca agcgatcgtc ggagtcgatt gagaacaccc gcagcgattc gaaccgccct
     6061 cacccccggc ccctctcccg ctaaacgggc gagggaagat catgggatag gctcttagct
     6121 gtccaatcat tacgacttgc ttgcttggaa aggaatacgt cgcatgatcc ggcaagaact
     6181 gattatcctg tgtttggcac tcgttgtacc cgcgacgcaa cgcacttggg cgatagaact
     6241 gtcgggcgtt ttcgtgccaa aattcactgt ggaaaaggcc cccgagctcg aaaagtggtt
     6301 tgatgccagt gacaagtggg gaggggccga cggcgtgttc tcgatccctc tcgacgagca
     6361 ccggctgctc ttcaccttcg gcgacacgtt taccacccgc caacggcgaa agatggtcaa
     6421 taactcgatc gccattatgc aattggaagg gcgaacgccc gttgacgtgg cattctactt
     6481 tgggcaaacc accgatggcg agatacgctc cttcgccgtt cccgaagacc accgcgggct
     6541 gttctggctg gcggccggct ttcgggatgg ggaccggctc cacctgtttg ccgcccatat
     6601 cgagctgacg cccatgggtg ggcctttcgg cttccgtcag atcggggaag cccttctcac
     6661 cgtgaacaat cccgatgacc cgccaccgca atggcacatc cagcagcaag ccctcccgta
     6721 ctttcggagc gacgatcaag ggacggcggt gatgggttcg gccgtatgtg tgccagcgga
     6781 ggccaattcc cagtacgtgt acctttatgg cttcgaagag gaaaagacgc cgctttttcc
     6841 catcaaacat gccatcgttg cccgcgttgc caaaggtcgg gcagatcaca ttaacgagtg
     6901 ggaattctat tgtggtccga gccagctccc cccaaacgca cagccgcaag actttataat
     6961 ccgtgccgag aactggacga gggacttttc tcgggcgacc aggctggcca gggaagtggc
     7021 gtcggagtat tccgtgtcgt atgttccctg ccttcaagcg tacgtcatgg tctatacccg
     7081 gctcggcctt tcgcaggaga tcatgatgcg gagggcaccg actcctgtcg gtccatggag
     7141 tgaggccctt cggctctatc gggtgcccga tctgccagaa gatcaaaagg ccttttacta
     7201 tgccgccaag gcccatcccg cccttgccgc cgatgatgag ttggtgatca gttatgttgc
     7261 gaattcgttt gactttggac gtctggtacg gacacccgag atttaccgtc cctggttcat
     7321 ccgcgtgaga ttcattcccg agtaacacca tcagccccag ccccactgta aggcgggagc
     7381 cttttcatcc atggccgatc gacccgctgt ttgcaccggc ggctggcaac atgttcggac
     7441 gcgagggcgt ggggcaccag gtagggcaat ccgccctcat gtctcggagg cgaacggccc
     7501 tcggaatcat cccatttgat caatctgttc cgcgcaggac atctaggacc tgattgtgga
     7561 gcaggccatt actgccgatc ccgttgccgg aggtgaagac gggctgaccc cgccaatccg
     7621 tgaagatccc gccggcttct tcgataatcg gctggatggc cgccgcgtcc cagatttcca
     7681 taaggggatc aatcataaaa tcgatccgtc cggtgcccac caaaaggtaa ccgtagccgt
     7741 cgccccatgt gcggaaaagg cgtgttgccg attgaagtcg gtgaaaggct tcacggcggt
     7801 tgatcttatc gaagttgagg acttcgctcg tcacccaggc cgcctcggcg aggttctcgc
     7861 agcgtgaaac acgggccggt tggggcgggt cttccccgat ctgataccac gcgccgcgtc
     7921 ccttggccgc aaaaatgact tcgcccgtcg ccggaatccg atcgacccca atcaccgccc
     7981 gcccttccca ttccacgccg atcaatgtgg catacagcgg cacgccgtgg atgaacgact
     8041 tggtgccgtc gatggggtcg ataatccaac ggaagccgct acttccttcg tccgtccccc
     8101 attcttcgcc gacaattcca tcctgaggaa acctcttgtg gatggcctct cgcaggtagg
     8161 cttcggcctc ccggtcggcg agtgtgacag gcgaggcatc ggccttccgc tccacagcca
     8221 aatcggcccg gcgaaaatag cgcagcgtga tctctccagc tgcgcgggca gcctccacgg
     8281 caaaatccaa acggccttgt atctcgctgg cagctcccac gttgaatatc ctatccaatg
     8341 tcaaatggtt gcccagatcg gtttgcattc accaaaaccg ccattatcgc agaaataacc
     8401 aaacacatta taggggcagg cccctgtgcc tgcccatgat gtggccgcga tcaccggtcc
     8461 atttgggcaa ccacaggggg ttgcccctac gggcgttaac cgaccgggaa aaccatccat
     8521 cataggggca ggcccctgtg cctgcccatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn taggggcagg cccctgtgcc tgcccatggt gtggcccgtc caccaacccg
     8701 ttcgggcaac cacggggggt tgcccctacc ggcgctaacc gaccgggaaa gccatccatc
     8761 gtacgggcag gcccccgtgc ctggccacat atggcaacgc aacgctgcca gacatataat
     8821 cgtcaccggc aactgccggt atgacgctcg tcaacggatc atccaaaacc gattgcgggg
     8881 gccaattcag agcacaagct ctgtggtaat cgctttcaat cccaaggacg agcagcgtta
     8941 ctcgctgaaa ccgcaccgtc tcggaagtac acaacgcact gcaaatcaac acgtggcaaa
     9001 agataaccca acacaattaa cgttgtttct agtcgattgt ctgttttcgg agagtaaaac
     9061 atgactcttt gtcgcttcac ttgtccggag tgcaaacagt cctttacgat ggagcttcca
     9121 gatgataccg acctggcaaa agtcacctgc cctcagtgcc agagcatttt gaccgttaag
     9181 gtgccgagcg aacccccgcc gattccagag gtaccgacag atcaggaacc accatcgcag
     9241 ttggtcgtac cgcctccaat cccaccgact ttacctccgg aacgcgaagc atcgtccggc
     9301 caaattcctt tgccgtcgga cattcttacc caaggccatc gcaactcaga gatggccagc
     9361 tcgcccgaga caagcgtcgt cccgccggcg ctcgcaccgt atgcaccgcc gaccgccccg
     9421 gtctcggtag cggccgagca tgcctgtccc ctttgcggcc gctcggatcc gaaatacttc
     9481 gtccgcggta agatgcttta tgggcacaag gtctgcagga aatgctatct gtcgtttctt
     9541 aaccgccggc aattcgcctt cgcgttcgat attgtggcct atttcatact gctgttggga
     9601 gtgaatttcc tagaggaatt cacggtgggc tttgtcctca gcgcaaccca agctgaattg
     9661 attgagcaac aattagaagc gatcctactt gcgtttaacg ttcttaacta tgtcttgtat
     9721 gcgctttggt tcctctttaa ggatggcttc aacggtactt cgctcggtaa agcgatcatg
     9781 ggcctcttcg taatcaacga agccacaggc aaacgcattt cttttggagg atcggccaaa
     9841 cgcaattgga ttatcgtcct ccttggtctc gtgccgtttg cgtggctaat tattgccgca
     9901 atcatgggaa aaggatatcg cattggggat cggatggcta acacaaaagt ggtgtggaaa
     9961 aaatatgctc acagcccggt gtttgctccc aatcgtgccg ttttatcatc ccctgggtta
    10021 tgaagcacgc cgcctcttct tttaactttt gtcttgacgt catttcggat tactggtatt
    10081 taccgccaca ttgacggtcc ctgtgtcatg gagacgctag cgcccccttg gcgccgctgg
    10141 tgctatttat cgtcgatcag gagccaaatc gcgttgtcca cccgttggcg acgaaccctc
    10201 attttcgtag gtattagtct gaccctgatg gggattgcct gggcaccgtt ggcctacgcg
    10261 caaagggtcc gctttcccac ggcttccctt acccaagatc cgggccaatc gatctaccag
    10321 caggtcccac cggctggcgg cacaggcgtg tcggccccat cggcggcgtc cggaaccacc
    10381 gctcccggtt accaaagtac gatcacccag ccatccggcg tcccatcata cgcaccacct
    10441 gctggagtaa gtggcccgcc aatccagggg tatggcacga ccgcgcccgg cgtgacggga
    10501 agcggaccct acccgtcccc ttgggatccc attccaactg ctcccccgcc tactgggacg
    10561 gctggctcgg cagtgggtgg gatcaactat ccgccgggcc aagttcccta ttacgcccca
    10621 gcagggccga ccgtcccgcc agcgccggga gccgtctatc aggggacaac acccccgccg
    10681 cccacttggg atccctacgc ggtccctgga aatccgtcac ctagtgtgtt tccgcagggt
    10741 ggtatttttc cgggcgttca gggggacagc ttctacaata cgatgatccg tttccgcgaa
    10801 gccacaggct ttcgcttcac ctggattggg ggatctgatc tcggtgtcaa tgatttggag
    10861 ctttactcga cgtttgcctt tccttttttg accacatcgg ggccgccact gcgggtgacg
    10921 cccggctttg cattccactg gtgggatggg cctgtgtcgt caggcctgcc cgaatccgcg
    10981 gacatgccct cggtgaccta cgacgcctac ctcgacgccg gttggaatcc gcaagtctcg
    11041 ccggtcctgg ggggagaatt gaacgttcgg gtaggggtgt attccgattt taagcgcgtc
    11101 acctccgaca gccttcgact tcaaggggcg ggatttctga ccttggccct cacgcccaat
    11161 tggaaactca aaggcggtgt catctatctg gaccgcgtac ggctaaaaat gcttccggca
    11221 ggcggggcca tttgggtcga taatccgcag aatcccacca tggaacttgc cctagtcttc
    11281 cccagtcccc ggtttgccat gcgactgccc cagcaaacga cggcggacgt ctggtggtat
    11341 gttcgtggtg actacggtgg gggttcctgg acgattaaac gcaccagtgg cgccaatatt
    11401 ggggctacgg aacaggtgga ctataacgat ttgaggctgg ccaccgggct ggagttttac
    11461 cgtttgcgag gtttgcgcgg ctatgccgaa gttgggttgg ccttttctcg cgaacttgtt
    11521 tacgaggccg gttggaccga taagtacaag cccgacgctg cggtcttctt aagtggcgga
    11581 ttggcgtttt agcccttcgc ctgcttcggc tgcccctttc gcccaggatc gtctcaccga
    11641 gcccgatcga tttgtaaggt atagtcttga caagggcctc ccttcccaat cccatttccg
    11701 caggaatcgc ctgcaatgtg cggtaatccg ctacaatagg attgggggga tggcggcaaa
    11761 aggtcagaag gtatcgtttt ctgacccagg atacgaacca gcgcactaga tagcagcgcg
    11821 gcatgagcac cgaacaacct ctcgatccgc aactgatcga acagacgcgg cagcaaatcc
    11881 ggcaattggt ggcggaggtg gccgagcttt cctcccgaac cgatctgacg cccgatcaat
    11941 actgcgcgga gtttttgccg cgggttgtgt cggcccttgc cgcctttggg ggcgtctttt
    12001 ggacagtcga aggccccggg cggattagcc tgacttatca gatgaacctc cagcagactc
    12061 gactggcgga agacgaggag gcccgacaaa aacacaaccg cctgctccaa cgggtactac
    12121 aaacgcggga agcgatcctg gtaccgccgc aatcaggcag cggagaaaac aacggcgagg
    12181 ccaaccctac ggatttcctc ttacttctgg gcccggtggg gacggagctg gaagtcgtgg
    12241 gggttgtgga gattttccaa cgtcccgatg ccccgcccgc agcccagaag ggttatctcc
    12301 gctttctcat gcagatgtgc gacatggcca gcgattattt caaatcgctg cagttgcgca
    12361 gtctgtcgga tcggcaaagc ctatggtcgc gattggaaga gtttacgcgg ctcattcatc
    12421 agagccttga tccggcagag acagcctaca cgctggccaa cgaagcgcgc cgcttgatcg
    12481 aatgcgaccg agtcacgatt tctgtgcgac gtgggcgtcg gcaagtcatt gaggccatct
    12541 cggggcagga cgtagtcaac aagcggtcca acgtggtgcg tctgttggga gatttggcga
    12601 cggtcgtcgc gacagcgcgg gagaacgttt ggttctgcgg cgacacatcg caattggcac
    12661 cacagatcga ggatgctctc cagcagtacg tcgatgaatc gcattccaag acggtgattg
    12721 tcatcccgct gggtcgtccg gcacctcccc catcggacga agccgaggcc gatcgaccgg
    12781 cggacccccc gcctccggtg ggcacgatga ttgtcgaaca gatcgaggac agccgtatca
    12841 cgccgcagct acggcagcgg gtggaggtgg tggcccaaca cggtgccgtg gccttggcca
    12901 atgccgtgga gcataacgag attttcctga tgccgctgtg gaaggcgatc gggcgatcta
    12961 aggtcatcac cgaagcccgc atgttgccca agacggcggc cgtgggaatc gtcatcgtcc
    13021 tccttctttt gactctgctt tttgctccct ggccgtataa gcttcatgcc cggggaacac
    13081 tggaacctgt cctgcggcgc gatgtgttcg cgggaattga cggcattgtg gatgaagtgt
    13141 tggtagtgca cgggcaacac gtcaatcagg gcgacatctt agcccgtttg cggaatagtg
    13201 aactgaaaca acagattctc gaaatcgaag gacagctcgc cgccaatcaa aagcaggtgg
    13261 ccaccatccg gcgagaactg gccgatctgc ggatgttaag tgcccaggag cggaaccgtc
    13321 tgacgggcca gcttgccgag ttggaggcac gccagatcag tcttgcggca caattggcga
    13381 tcctccggga aaaggaaaag gaattgaccg ttctcagtcc catcagcggc gatgttgtca
    13441 cgtgggacgt gtacaatcgg ctcatcaatc gtccggtcaa tcgtgggcaa gccctcatgc
    13501 ggattgccga cacgtcgggc gagtgggaac tggagctcca catgtcggaa gaccgaatgg
    13561 gtgagatcgc cttggcccgg caacaggtga aagcacaaga tccgagccag gacttgacgg
    13621 tggaatatgt cctggccacc gaccctggtc ggacgcggac cgcgcgggtg aaagatgttt
    13681 atgtgtcggc cgaggtcctt cccgaagagg gaaacgtggt catgatcaag gtggccgtca
    13741 acaaggatga tttcgacgcc acacaacttc ggcccggggc caccgtcagt gccaaagtcc
    13801 attgtggaac gcggtcgatg ggatacgctt ggttccgcga cctgatcgca tttatccaaa
    13861 cgcgtgtctt gttctatctg tggtgatccg ttgagttgag gaaatacgag accaaagatt
    13921 tcggagtggc atatggcttg gttgtgggtt gcggtgatgg ttttggcgac ggaaacgaat
    13981 gcaaatacca tcacgctggc caattgtccg gtctccatca tcgacgaagc ccaggtcccc
    14041 gcccgggtgc agggacaact ggttgaactg accgtcaaag agggcgactc cgtcacaaag
    14101 ggcgatgtgc tcggacgcat agatgacgca gtcctccgca agaccctgga ggtcaaacaa
    14161 caccaattgg cagccgccca aaaagaggcc gataacgacg tcaatgtccg ttatgcaaaa
    14221 tgggccaatc tcgtggccga tgcggagtat caacaggcag tggacgccaa caatcgggcc
    14281 ccgggagctg tcccccaggc agaaatgcgc cgcttgctcc tggaacgggg aaagacctcc
    14341 ctggccattg agcaagccgc ccacgatctg gaagtggcca agctcacagt tcaggtaaga
    14401 caggcagagt tggccgaggt cgaagaacaa ctggagatgt accgcatcgt cgcccctttg
    14461 gacggggttg tcgtgaaacg ctttttccac gccggggaat gggtcaagcc gggcgatacc
    14521 gtgttccata tcatccggat ggaccgcctg cggatcgaag ggatggtccg ggccgatcag
    14581 tacacgccag ggcaactggc caacgcgcgg acgattttca ccgtcaaatt gccccacctt
    14641 ggggaacaac gctttgaagg ccaaaccgtc tttgtcagtc ctgtggtaga ggtgaccggc
    14701 gagttcaccg tttgggcgga agtgaccaat atccgtgacc cgaaaaatgg ccagtggctc
    14761 cttcgcccgg ggctgacagg ccaactgacg attcagctta atccctaata tgtcgcttcc
    14821 tgtgccggca ggacaaaggg aatcaggaag ccagcggcat gggacaaagc tgaccgaaga
    14881 agattcctgt atcgtgtgaa atgaacctgc tatggtaacg cttgccgata gcctgctttc
    14941 cagttcagcg cggaagttat cgatccgcaa gcgggcggat ttggtggccc aaaagcagcg
    15001 ctatgaaggc gagacatatt atgtcatcaa agaccccgtc gggttgaatt acttccgctt
    15061 tcaagaggaa gagtttttta ttctcaacca acttgacggg caaagcagtc tggacgagat
    15121 caaagcccgc ttcgaagcgg aattccctcc ccagaaaatc accctggaag agcttcagca
    15181 gttcgtcggc atgttgcacc gcagtggcct ggtggtggcc gtggtaccgg agcaaggccg
    15241 acagttactc aagcgtcgtc gtgaacgccg caaacgggaa ttgctcagcg cccttggaaa
    15301 catcttggcg atccgctttc ggggaatcga tcccgaacgg attttgacgg ccatgtatcc
    15361 gtgggtccgc tgggtttttc atccggcctt tttgatcgcc tgtgtggtgc tgtggattgc
    15421 cgcccttttg ctgattgcct ccgaattccg ggtattcctc aaccgcttgc caaccttcca
    15481 tcagttcttc acaccaacca acgccctttg gctggccctg accttggccg tgacaaagat
    15541 cattcacgag tttggacacg ggctgagctg caagcacttt ggcggggaat gccacgagat
    15601 ggggatcatg ctccttgtgc ttaccccttg cctctactgc aatgtttccg actcctggat
    15661 gttgcccaac cgctggcacc gcgcggcaat cggtgctgcg gggatctttg tcgagctgac
    15721 actggcggct ctggcgacct ttatttggtg gtttagcgaa ccggggttga ttaaccattt
    15781 ggccctcaac gtgatgttca tttgctccgt aagcacgctc gttttcaacg gcaatccgct
    15841 actgcgatac gatggctatt acgtgttggc cgatatcctg gaaattccca atctgcgaca
    15901 aaaggccacg caactcctca gccgaacggc ggcccattgg ttcctgggga tcaagccccc
    15961 agatgaccca tttttgccgc aacggaacag ggccttgttc gtgctctatg cgattgccgc
    16021 ctcgatctac cgctgggtgg tggtgctttc cattcttctg tttttgaatc ggtttttcaa
    16081 accttatcgg ctggagatca ttgggcagat gattgccctg ggagcgttat acgggctggt
    16141 ggtccaaccg ttgtatcagg tcgggaagtt tttctatatt ccgggaaggt ggaatcaggt
    16201 gaagaagcct cgactttaca tgactttgac cggtttgggg ctggtgctgg cctttatcct
    16261 atttgtgccc ttgccctacc gagtgatgtg cacatgcgag atccaagcca aggatgcggc
    16321 ccccgtgtat gtggatgtct ccggacggtt ggaagaggtc ttggttcgac cgggacaggc
    16381 ggtccgcctt ggggattccc ttgcgcgtct gagtgactac gatctcgaga tccagatcgc
    16441 ccgactggaa accctccaac gtcagtatga agcccgcctg gccgttctgg aacgccgccg
    16501 ttctgaggac gaggccgccg ccaacgaact ccccgcgttg cgcgaggccc tccaaacggt
    16561 cacccaacaa ttggcagaaa aacaccgaga tcgcgatcgc ttgctgctac gggccccatg
    16621 cgatggcgta gtcctaccgc caccgtggaa gacggtgacc gcggacaaaa atgacccact
    16681 tcccgattgg tcgggtacgc ccctggagcc tcggaatatt ggggccttcc tccgcgaggg
    16741 tgagctcttc tgcctgattg gacagccgca aaacatggag gctatcctcg tcattgatca
    16801 ggccgacatc gagtttgttc agcccggcca gttggtccgc cttaagctcg atgagctacc
    16861 ctggcggaca ctcaggggca cgatcgaaga cgtggccaag cgcgaagtgg aagccgcgtc
    16921 accgcgattg gcgggcaagg ctggcggaga attggccacc aagaccgatc cgcgaacagg
    16981 cgtggaaaaa cccatctcta ccgtctatca ggcccgtgtc cctctcgtca atgacgagaa
    17041 cctgctgatt cctggtttgc gcggtcgtgc caaaatccac gtcgatcgcc agcattggct
    17101 gaccatcggt caacgggtgt ggcggttcgt aacgcggacc ttcaatttcc gtctctagga
    17161 caccgtcttc accccatttg gaggctatcc caaaaagtcc ccctctccct ccgggagagg
    17221 gttggggtga gggcaagcga acctcggaat cgattgagaa aaccagcagc gattcgagcc
    17281 accgtcaccc ccgacccctt tcctgccgaa cgcgtgaggg aagattatgg gataggctcc
    17341 aaacggggcg atagaccgtt ctcagaaata gaggtctcgt tcctctgttt cgcggatacg
    17401 acgaagccgc tcgatgaggg ccgtcttgcg agggccgtcg gggagtttgg ccaactcgtt
    17461 ttcgatcagt cgaaccccag tctcgtaggt ttcccgactt gcgtaatcga ccaaatactc
    17521 catgagggtg ctcagggcat tgggcgtgca aaaccgctgg ataaatcccg ggatggcgta
    17581 ctccatgaag tgttcgccgg tccgtcccag acgataacaa gccgtgcaaa aactgggaat
    17641 atagccatcg gtcatcaatt gccgaatgac ttcatccagg ggccgaagat cgcccaactc
    17701 gaattgctca cgttccaaga cctgggcgtc tcccacttcc gtgtaacctc cgacttcgat
    17761 gcggcttccg gcgtcgatct gcgaaacgcc gaactccatg acggcccggc ggagttcggc
    17821 cggttcgcgg gccgtgagaa tcagtccggt gtagggcacc gaaagccgta acacggcaat
    17881 gagccgtttg aaatcctcgt ccgagacgcg ataggtctcg tccaaagtga cccctgccgc
    17941 tcgtcgcaat cggggaaaac tgatcgtgtg cggtcccaca ttgtagtgcc gctgaaggtg
    18001 ccaggcgtga ctgaccagtc ccaggacctc aaaccgccaa tcgaatagcc caaacagggc
    18061 accaataccc acgtcatcac aaccggcctc catggcccgg ctaagggcat cgagccgcca
    18121 cagataatcc cctttgcgag tatttcgggg atgaaccttg gcataggtct cgtgatgata
    18181 tgtttcctgg aagatttggt acgtcccgat ccccgcggcc ttgacggtgg caaatccctc
    18241 gtggtcgaga ggcgccgcgt tgatgttcac gcggcggata ttcgcccggg gccgtttgac
    18301 cgcatagacc cgccgaacgc aatccgcgat gaactctggc gaataacggg gatgctcacc
    18361 aaagacaagg atcaacctct tatggccacg atccaagaga tggcagacct ggctttcgat
    18421 atcgtcgggc gaaagcgtcc tccgcaccac attcgggttg ctccgccgaa acgcgcaata
    18481 tacgcagtca ttggtgcatt cgctgccaat gtaaaggggg gcgaaaagga cgatccgatt
    18541 accgtaaaca tcccgcttca accggcgtgc ggcgtcaaag acttcctccc acaagtccgg
    18601 ctgttgaatg gtgagcagct tggccgtctc cggtaccgtg agcggctcct tgctcaaact
    18661 tttggcaatg acctcacgga cttcgcccgc ttgagcaggt tgctctagga ggctaaacaa
    18721 atatggctca tcaatgaaat ccaccgcact ttgacttaag cggcgggatc gtgtcacgtg
    18781 cgacatgtcg atacctcaac aatgcaacca tgattgtgca gcgactccgt cccatggaag
    18841 gccaggccta acgaaggccc aaccaatttc ctctggcaga acgcatagtc aatcctttgc
    18901 catccgatac cgataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacca
    19021 atttcctctg gcagaacgca tagtcaatcc tttgccatcc gataccgata attgggcgat
    19081 gtccccggac cacgtccgac gacgcgtcct aacgattcca cacgcatctg taaacaggcg
    19141 tgacactcgt ccggcgtctc ccgaagacac gccttattgg ggtaaatctc gtaacgaatc
    19201 ctgtattggg gtggcgtcaa attgggcatg acaacattcg ccccgcggac aagtcccagc
    19261 tcccgtccgt tcttgcgatt gagcgtggcc aaggcggttg tgctggggat gttcgcccgc
    19321 ggacagagaa tccgcgacaa cgcgatcacc ttgtacgtca tcaattcgct ggcaggcacc
    19381 tgctccccgt cgggaaggat cctggcaaag gccggatcgg ctaggggtgt ccgcgggtgg
    19441 acaataaatg gcccaacgcc gatcatgtcc aagtccaatt cccggaatag ccacagatcg
    19501 ttcgccaatc cggcataggt ctgtcccggt aggccgatca tgacgccgct gcccacctca
    19561 tagcccatct cacgcaagcg cttgagcagg taaagccgat caacggtgga gcccggtttg
    19621 ggtgggtgat agcgctgata caactccgct tgcgatgtct cgaaacggag gaggtaccga
    19681 tcggcccccg cctgccgcca cgcccaaagc tcttcttcgt ctcgttcgcc caaactaagg
    19741 gtgatggcaa ggcccgtggt ctctttgatt cgtcgcacaa tctccctgac gaagtcgcgc
    19801 gtcagccccg ggtcttcccc ggcttgcagg accacggtgc catagcccag cgcggctgcc
    19861 tgtcgggcac aggccaaaat ctcgtccccc gacatacggt accgcactag ctccgtattg
    19921 ggtgcccgca ggccacagta cgcgcataac cgaacgcaat gattcgaaat ctcaatcaat
    19981 ccccgcaggt gaacctcccc gccgacgaac tcctgacgaa ccctgtcggc ccactgccaa
    20041 agctgggaca gcctggctgg atcctcctcc cgtaaccacg tgaggagttc ttcgcgtgtt
    20101 agggcaggtt cttttaccgt ggttccaaac ataggggagt tttccccctt catttacgcc
    20161 gcatggacaa gttagtcttt tctgcaaaat ggcttcgagt tccgcgccgc ttggcaaatc
    20221 tagagcgcct tttgaattat acgactccca gccgagagaa gtgcttcctt gccctctcct
    20281 cacctttccg ccaagggcac aatcgtacgt cgcgcggtga tcattcttcc cttttttctc
    20341 aatggcgggt aacctcaaaa aagggaggtt cgtacggtct gcgcccaatc caagaccgat
    20401 tgccacacac cggccccgga aggatcgttc tgacctgcga gctgcccgtg gttacctgat
    20461 tgcgtcttag ggaactaccc caagagggat tgatatatgc cgcgtcgaaa tcttcaaatc
    20521 ctcgttttgg ccattattat ctgtcttctg tgtgcctacc ggagttcgcg atatacccgg
    20581 gttttgacgt acgcgttgga acaaatccaa tttaaggccc tcgaaccgcg atcgccgcag
    20641 caacttacag aaggtgccct ccgcggcatg gttggtgtcc tcgacccgta ttcaagctat
    20701 atcagcgagg aagacctacc caaattcgaa gaggaactcg accgccagtt tgccggtatc
    20761 ggcgtggaaa tcttcctcga cccacagagc cagcggttgt gcgtggccag ccccttaccg
    20821 aataccccgg cacaaaaagc cgggatcaaa cccggcgatt ggattgtggc catcgatgga
    20881 cagtccaccg aggggatgac ccttgaggag gccgggaaga agatgcgggg tgaaccgggg
    20941 acccaggtcg tgctttccgt acaaaccccg ggcgaagaaa aaccccacga ggtgaaactg
    21001 acccgcgaaa tcatccgcgc ggaaacaatc cttggcgata cccgaaatcc cgacggcaca
    21061 tggaattacg tcctccaaag ccatcccgag atcggctacg ttcgtatctc gaccttcgga
    21121 gaagaaacga ccgcccaact gaaaaaaatc atcctcgatt tgaaaaagaa aaacatccgc
    21181 ggattgatcc tggacctgag ggacaatccc gggggaaggc tggaagaggc cattgccatg
    21241 tgtgatctat ttatcgagtc cggcgtcatt gtgacgacgc gctatcggaa tggtcaaatc
    21301 aagcgacaat acaccgccag cgggaaaccc gtttgtcccg acctcccgat gagcgtcctc
    21361 attaaccatt acagcgccag cgccagcgaa atcgtcgccg cctgtttgca ggatcatggg
    21421 cgagcgacta ttgttggcga gcggtcgttc ggcaaaggga cggtgcagga gctcatccaa
    21481 cttgaggcag gcctcggtat gctgaaactc accaccgcgg cgtattggcg gcccagcggc
    21541 aagcagattc accgctccgc taatgcaggc gaagaccagg aatggggcgt caggccggac
    21601 cccggcaagg aggtgaaact agatcgggcg gagcaagata tgctagcgcg atatcgggca
    21661 ttgcgcgata tttacctccc gccggggagc cctaaacccg ccggcttcga cagtatcccg
    21721 gcagattttc gcgatcgcca actggaagtc gccgtggaat caattctcaa ggggcaagaa
    21781 acttcagcaa aatcggcaga ataaaacggg gttgcctgac agggaaaagt tgcggataaa
    21841 aagacttttg tggaataaca gcgaacgtgt caaagaggtg acgtggctgc acaggtgaag
    21901 tcccaagagt caacattatc ggaaagttgg ggaaatcgcn nnnnnnnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnng ccgtcctgga ggccgtttcc gcggacgatg tggatatggt
    22081 cctttcggct tttgtgggaa tcgcggggct gagggccacc tgggccgcct tggaggccgg
    22141 gaaaactgtg gccctggcca acaaggaaag tctggtggtg gccgggtccc tcatcacgca
    22201 attggcccga aagcgaaagg cccaaatcct tcccgtggac agtgaacatt cggcgatctt
    22261 tcaggccctc cacggcggcc aggaacgaga ggtacggcgg attatcttga cggccagcgg
    22321 cgggccattc cgcagtcgaa agctcaccga gctggcctcc gtcacggtgg aagaggcgct
    22381 ggcccatccc acttggaaaa tgggaaaaaa gatcacggta gattccgcca cactgatgaa
    22441 taaggccctg gagatcattg aagcccgctg gctatttggg gtggaggccg accaaatcac
    22501 ggtcgttatc catccccaat cgatcgtcca ttcgatggtc gaattcgtgg atggctcggt
    22561 gattgcccaa ctgagtcccc ccgatatgag acttcctatt cagtatgcgt tatactttcc
    22621 tgaacgacgg cccggcatcg cccgcaaaat cgactgggcg acggccatgg atttgcgatt
    22681 tgagccaccg gatttggagc gatttcctgc cattcgcttg ggactggagg ccgctcggat
    22741 cggggggacc gccggagccg tcctgaatgc cgccaatgaa gccgctgtag aatgttttct
    22801 tcagggacgc ctgcgatttg atcagattgt cccggtgtgt gccaaggtac tggaagccca
    22861 cccctttgac ccatcccctg atcttgacgc gatattcggt ctagacgcct gggcccgtaa
    22921 ggaggttgaa cgttggatat aaactcgctc ttttttgctt tcctgggtgg tgccgcgtgg
    22981 ctgtacgtgc tgatggtgat cgtcggcctc gggctgatca tctttgtcca cgagttaggt
    23041 cattttgtgg tggccaagct ctgtggcgtc aaatgcgaga agttttatct gggattcgat
    23101 ttcttcggcc tgcggctttt tcgttttcgc tggggcgaaa cggaatacgg aattggcatc
    23161 atcccacttg gcggttacgt caagatgttg gggcaggaag ataaccccgc ccgactccgc
    23221 gaggaatttg aacgcgccaa ggccgccggt caggaaggag ccaagtcctc caccggcgaa
    23281 tcgttggcca acatggaaga ggccctttac aacccaagga gctacctggc caagagtgtc
    23341 ccccaacgga tggccatcat ttccgcaggc gtggccatga accttctttt tgccttcatc
    23401 gcggcggtgc tggcctattt tatcggggtt caagagattc cctgtggtgt gggccaactt
    23461 gcgccgggag agggggcatg gcgggcgggg attctgccgg gtgacaagat tctggagatc
    23521 aacggtcaaa agacccaccg cttccaagat ctccagcagt ccatctcgtt ggcgcggcgg
    23581 gggcaagtcg tcaccatgcg gatcgaacga cccaccccaa acggtctcaa ggtattggac
    23641 ctagaactta tgcccgaccg aatccgcgtg gtgccgacca ttggcgtgac aaatccactg
    23701 ttaccgatcc tggccgagcc gccgtgttat ccggggagcg ctgccgccga agttatcacg
    23761 agcttaccgc caggcgccac ggtcaaggcc atccatggcg agcccgtcac tggcttcgcc
    23821 gacattgctc gatcctgggc caagtcctgg ggtcagcccg tggagctaat gctcgagaaa
    23881 aaggctgcga gcggaacgcc tctcgagccc cagaaggttg tcctccagcc aacgccggtt
    23941 cgtgacttgg ggttgatttt caaggtggga cgaattgtcg cggtgcgggc gggctccccg
    24001 gctgaggttg caggggtcct ggcgggcgac gaattgatga gtcttacctg tcccgaaaac
    24061 gtgaactgga acggcaactt catgcggcta ccttacgccc tccaaaaagc cctccgcaat
    24121 cggcccgagg gaatccctct ccaactcacg gtaaagagat cgaacggctc ggagaaggca
    24181 ggcgtcgcct tggtggccat gcgttcggcc accgccgccg ataaaaacag ctccaacgaa
    24241 ggcactccgc tgaagcttga cgtagtggcc aaaaccgccg agttcttcgc cgatctcaat
    24301 acgggcgata gtcccttcga gatccctcaa cttggcattg cagtagagat tctgccccag
    24361 attgaggcgg tagaaccgca ttcgccggcg gcggctgccg gattacagcc gggggacgag
    24421 ctaatcagcg tgaccgccat cccgcccaac ctcgaccccg aatcaaagat cggagagctt
    24481 gccagacact tctccatgac gaagtttacc gtccgcttcg atcaaccgcg aggtgaagaa
    24541 aaaggcacct ggcccttttt cttccgggaa ttgcagagga tgccgacggg gactgaggtg
    24601 gatctcgtgg tacgacgtcc ccagccggac cctgcccagg agtatcagga agtaaaggtc
    24661 catctcgtcc cgcgcgtcgc ggacgattgg ttcttggccg agcgcgactt gctttttgaa
    24721 gcaatgactt tcgacctcag ggcggcaggc ttgggagaag ccatcaccat gggggccaca
    24781 gagacgtgga cttcggtaac cctgatctat cgcgtcttga acagcctggg gagaggtgat
    24841 gtctcgcccc gggctttggt cggccctatc gggttggtcc aggcggccta tcgggtggcc
    24901 tcgcagggcc tcggtcggtt tttacttttc ctcacgctgt tgagtgccaa tttggccgtt
    24961 atcaatttcc tgcccattcc ggtcctcgac ggagggcaca tggcgtttct tatttacgag
    25021 ggaattcgcg ggaaaccgcc gagtgaaacc gtctatgtcg gcctatccta tctgggattg
    25081 gcaatcatcc tggggatcat gctctgggtc tttggcctgg acctgggctt catctcccgc
    25141 taagggcctc gcaggcaatc gggcttgcca cgtccaaatt ggagtgatgg gaattaatga
    25201 cagaccttgc gagctccgca ccacgcttgg ttgccattgg gatgggatcg aacctcggtg
    25261 attcgtggac gatcctccgc caagcgtgca aggccctcag cgagttgccg tgcacccagc
    25321 ttcgcgctac cagccgcatc agacttaccc agcccattag agtgtcagac caaggctcgc
    25381 ccttttttaa tgcagtcgcg cttctggaga cggcccttcc tccgcgggaa cttttggatg
    25441 gaatcatggc gatcgaacaa aggttcggtc gcgaacgcaa tcgtcactgg gcatcgcgga
    25501 cacttgactt ggacatcctc cttgatgagg ccggcccgtt ctacgaccag cggctcctga
    25561 ttccccatcc gcggttaggg tcgcggctat tttttctcgc ctgcttggaa gagctccttc
    25621 ccgattggcg acacccctgg tgcggtttga ccgtgcggca aatggttgag attctttcca
    25681 cccggccacc atattgcgtg ttaatttggg agacatttct gcccacccat gaggcatcga
    25741 gcttgacgac ccacagcggc gagattcagg cgggcgaggc agactgggcg gccctatggc
    25801 aggcgtgcgt ggccgcgaac aaggtgtttc aggcaggatg cccacatctc gaggactcgg
    25861 ggggatcagt ctcggccggt gtgcacctta cccacccacc ggaaagttgc atatttaacg
    25921 cggaggaggt tcaacaccgc ttggcgatgg gtcaatcgct ggcggagatt gtgtcattcg
    25981 cttggttgga gcatcgcaag caggatcata atgggcgaga cactcccctg ccccgcgtga
    26041 tgatcctcgg tccccgggcg aaggacttgt tgtggccgca tgaggcaaac gcatcagatc
    26101 ggccgcttgt gagtccctcc ttcgaattgg caaatctctc ttggcttcgg gatcaggtta
    26161 cacccgtggt gttctccgag gcggatgatc cgcgaacttg ggcggagcac atcattgcgg
    26221 ccattgatgc ttccgccgaa gttgggcacg tgttgggcga tttgggataa gggatgctcc
    26281 taagcccaaa aagcccccct ctcgcggagg gacaggggga ctttttagga tagaccttta
    26341 gggtgccaga ggtacggtc
//
LOCUS       DAOSUT010000046        26057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1225_length_26057_cov_9.466656, whole genome shotgun sequence.
ACCESSION   DAOSUT010000046 DAOSUT010000000
VERSION     DAOSUT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_26057_cov_9.466656"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..759
                     /locus_tag="PLD05_05905"
     CDS             289..759
                     /locus_tag="PLD05_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOP77005.1"
                     /translation="MMSQTGEVSKTSIKEILLAKGSHVYTIDPDATLQDVVEELVQHN
                     IGALVVCHRDPDRGEKILGIITERDILRRCAYSTGPLSEVRVSEVMSRNLITAQPDDT
                     VAHALQLMTIHRVRHLPIVLDGQLVGIISIGDAVKAQLEHLMLENRFMKDYIQG"
     gene            complement(774..2075)
                     /locus_tag="PLD05_05910"
     CDS             complement(774..2075)
                     /locus_tag="PLD05_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOP77006.1"
                     /translation="MSWLRLVLFVAVPFMGSLLYALEAPSVNYDESKVPAYTLPDPLV
                     CEDGTVVRDAATWMQKRRPELLQLFQTHVYGRSPGRPADLSFVLKSRDDNALGGKAVR
                     KEVRIHFSAKKDEGPGMDLLIYLPKEAPRPVPVFLGLNFRGNHAVHPDPAISIPTSWM
                     RPGEGVVNNRATEAGRGTAASRWPIEMVLEHGYGVATAYYGDIDPDFDDGFQNGVHPL
                     FYKEGQTRPAPDEWGSIGAWAWGLSRAMDYFETDDDIDEKRVAVLGHSRLGKTALWAG
                     AQDERFAIVISNNSGCGGAALSRRRFGETVARINTSFPHWFCDNFKKYNNNEDALPVD
                     QHELIALIAPRPVYIASATEDLWADPRGEFLAAKGADPVYKLLGTEGLPVEDMPPPDQ
                     PVMGRIGYHLRTGKHDITAYDWEQYIKFADKHFGRTTSKVE"
     gene            complement(2390..3097)
                     /locus_tag="PLD05_05915"
     CDS             complement(2390..3097)
                     /locus_tag="PLD05_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP77007.1"
                     /translation="MPSLILEQITKTYVTSAEPVEVLRGIDLSVPSSDSLAIVGPSGS
                     GKSTLLNIIGGLDRPTSGRVIVDGEDITRMSELSLSKFRNQKIGFVFQDHHLLPQCSV
                     LENVLIPSLAQGRPSKEHLQWAEYLLERVGLSHRAHHRPSELSGGERQRAALARALIL
                     RPVLILADEPTGNLDSVAGRKVAELLLELQAENQAILITVTHSPFVASMMSRRLHLED
                     GRLVERPQNVSLTSNQL"
     gene            complement(3193..4173)
                     /locus_tag="PLD05_05920"
     CDS             complement(3193..4173)
                     /locus_tag="PLD05_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP77008.1"
                     /translation="MTDPHSVTRRGFLGSLSAGGIMAGPTLLHFAQVTDSEPQVASSY
                     RLIDTHVYLFQWPFRLIHSAETAELVRSLKNAGVTCAWAGSLESLLHRDIASVNARLH
                     EECRKYGDGFLVPCGTINPMLPDWTEDFRQCVENYKMRVLRLFPGYHGYTLDIPEVGR
                     LLALAHKCEIGIQITIEMEDVRAQNPLLPVKPVEVTPLGRWLEDFPALRLMLLNCHRA
                     VPPQQLAKLVTIGWVYVDLGMLEGIAGLERLTSVIPIERICFGSFAPVFYFESALLKL
                     AESHLPGGSLDAICYQNAETFSHRQPPYPRKGGFPMPYQRTCAVWLASSG"
     gene            complement(4170..5012)
                     /locus_tag="PLD05_05925"
     CDS             complement(4170..5012)
                     /locus_tag="PLD05_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP77009.1"
                     /translation="MDFSPRLADIKQRLFPFPLEIWDGHTHLLGLPGKTPEERLERLL
                     ECASRLGIGRVCVFMGTKWQYDPTPDEMQEANNEVRRAIERFPSRAFGFVYVNPNHQV
                     ASLDEIRRHVRDGPMVGVKLWVARRCCDSALDPIVALAHDCDAVVLQHTWIKTTGNLP
                     GESTPHDLACLARRHPDAVFIAAHSGGGNWEIGLRAFCETPNVYAELSGSDPTSGFTE
                     MAMRLLGPRRVIFGSDAPGRSFATQLGKVLGASVDDDTKRLILADNLKKLLAPILKKK
                     GVPV"
     gene            5552..7552
                     /locus_tag="PLD05_05930"
     CDS             5552..7552
                     /locus_tag="PLD05_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77010.1"
                     /translation="MDRQRHAGIGLSFWPRIFLIIIVSLVAIGQTTIGAELAEQDRHT
                     SLQSGVVNAGEVKPTATRPNILWLTLEDVNCHFGCYGDSYATTPNIDRLAKEGIRYTN
                     AIAGASVCTPCRSTLITGMYSVTLGSQHLRSPIRLPKEVRCFPELLRRAGYYCTNNVK
                     QDYNFECPPGVWDESSPRAHWRNRPDGQPFFAVFNTMVTHQSRVRMSDDEFAKDIATL
                     EPVTRHDPATVPLPPYYPDTPGIRKEMARYYDMISAADRWVGKHLQDLADAGLKEDTI
                     VFVFGDNGAGIPRHKRALYDTGVRVPLLIWFPKKFAHLAPAQPGSVVSEIVSFVDFAP
                     TILRLAGVEPPELMQGQAFLGTHGGSRLHAYLFRDRVDEVFELSRGVRDQQFKYIRNF
                     MPHRPYMEISEYCEPAQMVRDLRRLAAEGQLSGPAALYLRETKPIEELYDLKNDPYEL
                     HNLTADPKYAQILQRMRQELRTYVLSLRDTGFLPESDMFRRAGDQTIWEMAQDDEKYP
                     LARIYAAADAVGRPEKSYQDFEAFFADADPAVRYWGVIGLMSMTELPQRAVSRLYELL
                     DDSSPEVRIASAEALCRLQKPEKAVQVLGKELRSSDPYVRLLAASTLAVINGHAAGAR
                     DPILEIIGEKGTGDMWMFTQWALARCCRQLNWPIPAHVPTQY"
     gene            complement(7659..9011)
                     /locus_tag="PLD05_05935"
     CDS             complement(7659..9011)
                     /locus_tag="PLD05_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP77011.1"
                     /translation="MLTRCCLRFPAWSISVLCVLVWATGLSATFGLNEDLRETPIVRA
                     IRSARPAVVNIRGEKSLITTAGNSTRPDAGRRVNGMGTGVVIDERGYIITNHHVVDGV
                     REIEVTLHDGTRYIGKLVARDPKTDLAIIKINPDQPLQVIQIGTSADLMPGESVIALG
                     NAYGYEHTATRGIISALHRAVQVSDSQYYDDLIQTDASINPGNSGGPLLNINGEMIGV
                     NVAVRAGAQGIGFAIPVDRVVEAAAELLAQINDQKLYYGVRFRSAPPTGPLTVSEVDP
                     NSPAAAAGLQPGDVITEVANRKVRNALDFQRALLEKSADDVLPVVVTRNGEGNQQLML
                     TLSKPKTATLDDEIWMMLGLRLEPIPEETFRQKFQTRYRGGLLITEVRSGSPAAAQGL
                     RPGDVLVGMHLWETISLDNVEYIIHRPDFASFLPLKFFLLRGNETYYGYFSVALRSEQ
                     "
     gene            9694..9924
                     /locus_tag="PLD05_05940"
     CDS             9694..9924
                     /locus_tag="PLD05_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77012.1"
                     /translation="MALTRKTGRVGRGKNFACDDPMEAVKDSGKPSGPKSASGGGVTG
                     KPFPERKRGDPRQVSDAPRQGEQGRQLAVRER"
     gene            complement(10242..11333)
                     /locus_tag="PLD05_05945"
     CDS             complement(10242..11333)
                     /locus_tag="PLD05_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77013.1"
                     /translation="MKSTDWLSASSGGNGNPIPVGVIGAGRLGTFHARKLARLRDVRL
                     VGVADPLVDRREAVAKECRCSAFADYRDLLPHCAAVVVAVPTSLHHRTAMDCLEAGVH
                     VFVEKPITETPQQAHQLWSTAQRKNLVLQVGHIERFNPAYRAVQPLLGDIQLIEASRC
                     GPFSFRVTDVGVVMDLMIHDLDLILAMVRKPVLRTDSVGVSLLGRHEDLAIARIEFED
                     GPVALLKASRIEQEPIRTMRIWHRAGQAIVDFARRTASVCEYPEEIRSGAFRIEELSP
                     QELNSLKETFTSRFFPWRQLEIASYDPLEAELRNFIDAVRTGTLPLVTGGDGLAAVQL
                     AERILDGIRARESLIRPIVPIPRSLPKAA"
     gene            complement(11384..12259)
                     /gene="lpxC"
                     /locus_tag="PLD05_05950"
     CDS             complement(11384..12259)
                     /gene="lpxC"
                     /locus_tag="PLD05_05950"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOP77014.1"
                     /translation="MLSLRPQQTIARAVAVEGFGYWSGTDVRVEFRPAPPNSGIVFVR
                     EDLRHRPRIPARLEYRMETPRRSSLEFSGARVEMVEHVMAALGGLQIDNCEISVTAAE
                     MPGCDGSALPFVEALLQAGIIKQSAPRAVRVLTRVVRVEDRNSWFEARPGIANECFMR
                     YLLDYGPDGPIGRQSFGLALTPESFCRELAPCRTFMLKEEAEWLLSQGLGRRTTPRDV
                     LVFDADGPIDNQLRFPDECVRHKLLDMVGDLALCGCDFVGRFSAYRSGHRLNAEMGRN
                     LLNQFPLVERIRSCA"
     gene            complement(12473..13168)
                     /locus_tag="PLD05_05955"
     CDS             complement(12473..13168)
                     /locus_tag="PLD05_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOP77015.1"
                     /translation="MLQKKWLALAAAGLVVAGVIVYQLRAQQVTTPTAPVRSATLVRN
                     IAVVDVEYIFKNHLRFKQEMSVLRSEATAVEQWAQREQEAIKSLAEQLKDHQPGTATY
                     NQIDDTLVKRQKDFEAEIIKKRKEFLLREAKLHYATYQEIVNEIQYLAAANGIAVVLR
                     FNGQQVDPNSPDDILRYINQLIVYQAPELDLTPLVLQRLNARAQSTTSSASSTSSPVV
                     PGQGNFPRPGTLR"
     gene            13650..15509
                     /locus_tag="PLD05_05960"
     CDS             13650..15509
                     /locus_tag="PLD05_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOP77016.1"
                     /translation="MELTRYQVTVLPLGRTIEVLAGSRFVDIARTLGLAISLPCGGEG
                     VCGKCRVWVRSGMAEPTAADRRHLTESELEQGQRLACQLAVRSDVVIEIPETLLLRAS
                     HQILTKTGEVRLEKDTPLVTKRTVNLTETPSATDLVAIVRHALPQVGLPLCVLQDLSQ
                     QLLEIPSTRLAVLAEGCLLALESPEEASPTLGVAVDLGTTTLAASLLDMTTGAELAVC
                     GRLNPQTEFGDDVISRIQFASETPWGTQRLQHAVVSAINEMIEELIGQAGVDRQMIYQ
                     VVCAGNTTMQHLFCGLSPQHLGRFPFRATTLEAQRFFASEVGLNVHPRGRVYVFPAIG
                     GFVGGDTVAGIVTTGLDQSRSPALLVDLGTNGEIVLWDGQVLRAAATAAGPAFEGARI
                     KCGMRATEGAIERVWWDETAQQLRYHVIGGTDPVGLCGSAILDVLALLRQWGIVTAEG
                     RLQGGAALRRTLPEDLAHRLIDDQGTPAFIIVQNSRGDARGDIVLTQRDIRQLQLAIG
                     AIRAGTEMLLRSAEIAPEDLQIVYLAGGFGNYIRRRNAQLVGLLPRHIPTERIQFCGN
                     ASLFGAKMALLSREVCRRAEDVSRKVEHIELASHPDFHWKFAEAMIFPDCQPL"
     gene            complement(15572..15904)
                     /locus_tag="PLD05_05965"
     CDS             complement(15572..15904)
                     /locus_tag="PLD05_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOP77017.1"
                     /translation="MGLSLTPVAASEIKRIIEEQKLEPGTVLRMAIGGGGCSGLQYSL
                     GFDTEYDPKLDARFESNGVSLVTRKKFALHLDGTVIDFLDGPAGRGFVINNPNYPRGG
                     GCPGCGGH"
     gene            16354..17277
                     /locus_tag="PLD05_05970"
     CDS             16354..17277
                     /locus_tag="PLD05_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOP77018.1"
                     /translation="MELQLIDYFRQRIPQYPCVRLGIGDDAAIIDLDIPQCVVTTDIL
                     MDGVDFRLSEVTPEAIGRKALAVNLSDLAAMAASPKACVVGLVLPEKGGVEIAKRLYD
                     GLLPLARDFQIAVAGGDTNSWEGPLVISVTAIGLPSPGGGLCRAGAKPGDRIIVTGDF
                     GGSLLGKHLTFTPRVWEAIYLYRSYEIHAGIDVSDGLALDLWRLTVESRCGAVIQAEK
                     VPISEASHRMVAQVKSRMKPLDHALHDGEDFELILAVPPAEAARMLRDQPLSVPLTDI
                     GEFVPDRGLWLEKGKKRTPLEPKGYQHKFSL"
     gene            17490..18014
                     /gene="tsaE"
                     /locus_tag="PLD05_05975"
     CDS             17490..18014
                     /gene="tsaE"
                     /locus_tag="PLD05_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOP77019.1"
                     /translation="MITRSEEETRQLGALIAQCLPRPSVIGLMGTLGSGKTRLVQGIA
                     HGCGVREAAVISPTFVLIHEYPGDVPVFHFDVYRLKDVAEFLDLGPEEYFQRPGISVI
                     EWADRVEEYLPPHRLIVEIEVVSESDRCFLFRPQGQEYEKAVDQIIEMWRRAAGDPMR
                     KLVGKDCPNSREEG"
     gene            18192..18542
                     /locus_tag="PLD05_05980"
     CDS             18192..18542
                     /locus_tag="PLD05_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77020.1"
                     /translation="MSRPDDEAFNLDAILGAGAPEVEGMPGAPPVSPDLAPPAAEAPS
                     EAPAEAKPPKPSLFARLRRIDIFTVMLGLSLLAIIIAVVMLAIELSRYQWDTSARSAR
                     QTVRWVTVTSEHLV"
     gene            complement(18704..19966)
                     /locus_tag="PLD05_05985"
     CDS             complement(18704..19966)
                     /locus_tag="PLD05_05985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOP77021.1"
                     /translation="MLNSEIVITGIGVVSPIGIGREEFTQSLSHRKSGVGPIGLFDSS
                     GLPVHSAAEVRDFDPRQFIPNRKSVKVMARDSQMGVAASILAYKDARLDSAGVDPERI
                     AVVLGADRICGALEDSKPTYEACLVDGQFHFDRWAPLGMPATFPLVFLKVLPNMIASH
                     ISIAIDARGPNNTIHHGDLSALLAIIEGASLLERGLADVVLVGGASSQMNPFDWARFV
                     SLGRFSRAVDNGRASPRPFDKHRDGEVRGESAAVLVLERVNHAKARGATIWGTIRGWG
                     RCYAPLDGDLNSFPSLSRAISLALQDAAIESKDVAQVNAHGLGTEEDDIRESHGIRRV
                     LSDVPVTAPKSYFGNTGAAGGAVELAASLVLGKDRLVPPTLHYDEADPRCPINVVRGA
                     PQPLASPFLVAVNWTLAGQAAALVVEVA"
     assembly_gap    20151..20250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20348..20887
                     /locus_tag="PLD05_05990"
     CDS             20348..20887
                     /locus_tag="PLD05_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77022.1"
                     /translation="MRTSKAFILLSAIFGLATIIGCRMCASPHDSCGPVVSAGSSGRG
                     CTSARAGSAFAVTAYSDHTDENSPGYASAPHVAGLDSDRRTVSSSKGPLPRSPHRPLT
                     PEDIDPFVKLGIPPENILSVTDRPADEPIAQETSPSPQTLVHADPKQPKAPATNADRE
                     SVTSAGWTRIGERTSGTLR"
     gene            21277..22878
                     /locus_tag="PLD05_05995"
     CDS             21277..22878
                     /locus_tag="PLD05_05995"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOP77023.1"
                     /translation="MSDSNRPDVKTSGVTMAEAPVRLGEMLLRMGPGMIIAAAVVGSG
                     ELILTPRTGAEAGFTLLWIILLGCVTKVFVQVELGRYAIESGQTTLAAINRLPGPRWR
                     VHWMLWYWVVMFVVSLAQLGGIAHGVGQALAIALPIDGSFRQLLKEQEEWDREAAQIR
                     RELEASFEQPKGSGSAGTASVDKKTVETEVAQRMQRPRPGYENREGLLTDDLIWAIII
                     CLVTIPILVYGRYGMIQWVCTILVVGFTLVTIFCVIGIQFKPEWAISWDDLREGLSFR
                     LPGTANRWLALSTALATFGIIGMGTNELITYPYWCLEKGYARFTGPRDNSPGWAKRAR
                     GWMRVMRADAFLSMVVYTFVTAAFYLLGAAVLYRSELIPAGSQMISYLSEMYVPVLGE
                     WTKAFFLFGAFAVLYSTFFSATAGHTRVAADAVAIVRRGRSQPDRPPQSWVTAFSIGF
                     PLFSLVTCLFYREPVTLIVASGFMQAIMLPMLAGAALYYRYRECHEALRPGLIWDICL
                     WISAIALLVAGLFGAGMGLHRLLAH"
     gene            complement(22875..24095)
                     /locus_tag="PLD05_06000"
     CDS             complement(22875..24095)
                     /locus_tag="PLD05_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOP77024.1"
                     /translation="MSEARLAQFSEIAALCRDDDPDFAVHVVEQLINWASSLGASDLH
                     LNPMADCLEVRIRLDGVLHRAGSLPNFAQRKIVTRIKVLADLLTYRTDIPQEGRIRLP
                     GVGPEIRVSTFPSLYGERIVTRFFPEETRFKVLNDLGFPEGIVQILRRALTETSGAIF
                     ITGPAGCGKTTTMYACLRELTEETIPRNLMTVEDPIEVAIPGVIQSQVDPRVGLDLAA
                     ALRYLMRQDPEVVAVGEIRDRTTAEVAVEASLTGHLILSTYHAGGAAEAIGRLLDMGI
                     EPYLIRSGVLVVVSQRLLRQVCECAEWTGDSGQMLGLPVDQVRVAKGCEECRHTGYVG
                     RFPIAEVLVLQDEAIGRAVLDRSDVITLERVAMERGMVTLWENAITAVKEGRTTPLEV
                     RRVLGWGDYWKLTA"
     gene            24337..25395
                     /locus_tag="PLD05_06005"
     CDS             24337..25395
                     /locus_tag="PLD05_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77025.1"
                     /translation="MSEAEKPRPENLHNRCEGETDRNAIDSENVMESAADRDSDGSGT
                     EILKNELVPMDHDEEKRSAFLTGEEILAPEAVEKVEVAAAESSDAPENDSGLELPEDE
                     LLDLPRSTDARVISCTDTDVSSAESADQTGELAQQEALEVETSAVSEPAVTEMTAEEQ
                     PAAEGPSDQEGTGATEVVTTEPEAESTILMVRVPGDERWFPLSEVIVQTTGEPFGESA
                     ARWISRLYRQSLESTPEGPEMSSGPSTAVGEVVFPSITTAPEPTDYRERLKKAAPKKS
                     IVKELLGIVLGGIGGLLIAYYALNWFGGPRYDFAKIPLPGLHHTYKHAPPWLKDLLEG
                     KYWTTSGEDSSADEQVEP"
     gene            complement(25418..25873)
                     /gene="rpiB"
                     /locus_tag="PLD05_06010"
     CDS             complement(25418..25873)
                     /gene="rpiB"
                     /locus_tag="PLD05_06010"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOP77026.1"
                     /translation="MRIVVGSDHRGSTLRHKVIAYLSRLGQEVIDVGVDSQNGVDYPD
                     VAAEVARRISGSEGERGLLICGTGLGMAIVANKFPGVRAAPCHDELMVEIARRHNDLN
                     VLCLAADLVGELLAIRLIDVWLQTEFEGGRHARRLQKIAQIESNGHYRK"
BASE COUNT         5768 a         7020 c         7348 g         5821 t
ORIGIN      
        1 tgcaactccg ggcccaacac cggtgggcga tattctcgcg gtagggggaa atttggacgg
       61 ccctgtcatg cacagggtgt gacgggcttg actactcttc caccggtcga gacgccctct
      121 agtccttaat ggtctctgtt gccgccttta gccgggtgcc agacggcgag cgacttgact
      181 tctcagttgg cgggttgaat aatcgaggtg gcattttagt ctgttggaac tggcgcgcga
      241 cgccagctct ccaccagcag tccgaggttt tggaccggga ggtcggccat gatgagtcag
      301 acgggagagg ttagcaaaac ttcgataaaa gaaatcctgc tggcaaaggg aagtcacgtt
      361 tatacgattg atcccgatgc taccttgcag gacgttgtcg aagaattggt ccaacacaac
      421 atcggtgcgc tagttgtgtg ccaccgtgat ccagaccggg gtgaaaaaat cctgggcatc
      481 atcaccgagc gcgatatctt gcggcgatgt gcctattcca cgggcccgct aagcgaggtc
      541 cgtgtttcgg aagtcatgtc gcggaacctc attacagccc agccggacga cacggtcgca
      601 cacgccttgc aacttatgac gattcatcgt gtccgtcatc ttccgattgt cctggatggg
      661 caattagtcg gaattatctc gatcggggat gcggtaaagg cccaactcga gcaccttatg
      721 ctcgagaatc gtttcatgaa ggattacatc caaggctgaa cccgaatctc gtcctactcg
      781 accttggatg tcgtacgccc gaaatgctta tcggcaaatt tgatatattg ttcccagtca
      841 tacgctgtga tatcgtgttt gcccgttcgc aagtggtaac caatacgacc cattaccggt
      901 tggtctggcg ggggcatatc ttccacgggt aggccttccg ttcctaggag tttatacact
      961 ggatctgcgc ccttggctgc gagaaactcc ccgcggggat cagcccaaag gtcttccgtc
     1021 gcactggcga tatagacggg tcgcggagct atcagggcaa tcagctcatg ttgatccacc
     1081 ggtaaggcgt cctcattgtt gttgtacttc ttgaaattgt cgcagaacca atgaggaaaa
     1141 ctggtattga ttcgcgcgac ggtttcgccg aaacgccggc ggcttagggc cgctcctcca
     1201 caacctgaat tgttggaaat gacaatcgca aagcgttcgt cttgcgcgcc cgcccacaaa
     1261 gccgtcttgc ccaaacgcga atggcctagg acggctacgc gcttttcgtc gatgtcatcg
     1321 tcggtctcaa agtagtccat ggcgcgactg agtccccatg cccaggcccc aatcgaaccc
     1381 cactcgtcgg gtgcgggcct ggtttgaccc tctttgtaaa agagcggatg cacaccgttt
     1441 tggaatccat cgtcgaaatc gggatcaatg tccccgtagt aagccgtggc aacaccgtag
     1501 ccgtgttcca aaaccatctc gatgggccaa cgactggccg ccgtaccacg gcctgcctcg
     1561 gtggcacggt tattgacgac accttcgccg ggtcgcatcc aactcgttgg aattgagatc
     1621 gctgggtcgg gatggaccgc atgatttccc cgaaagttca agccaagaaa cacgggcacc
     1681 ggtcggggcg cctcttttgg caagtagatg agaagatcca ttccagggcc ttcatctttc
     1741 ttagccgaga aatgaatccg gacctccttt cgcacagctt taccccccag ggcattgtcg
     1801 tcgcgacttt taagaacaaa agacaaatct gcagggcggc ccggagaccg gccataaacg
     1861 tgggtttgga acagttgcag cagttctggc cgacgctttt gcatccatgt ggcggcgtcg
     1921 cgaaccaccg tcccgtcctc gcagacaagc ggatcgggca aggtatacgc gggcaccttg
     1981 ctctcgtcgt aattgactga tggcgcctct aatgcataaa gaagactgcc cataaagggc
     2041 acagccacaa acaagaccaa ccgaagccag ctcatgttac acctccaacg atcggtgaca
     2101 ttgttaggat tctgagaatg cactttcgac acgatacgtg tccactgtgt ggccgatagt
     2161 ttaacgagat tatggtcaga atacaacgaa cccaaattgt ccaaggtcgg cttctcgctt
     2221 tacctggctt gccaagcaag gtgtacggcg agtggcaacc agagatctag tcggccggtg
     2281 tgctcgttta ttgacagact tgctctcgca aatcagggca tagcatcgcg tgctggctaa
     2341 ctatatcaga acgctgatgt tgatgtgcgc tggaaaaaag tgacttatct cataactggt
     2401 tggatgtaag cgaaacgttt tgcggtctct cgacgaggcg gccgtcttca agatgtaagc
     2461 ggcgcgacat catggaagca acgaatggac tatgagtaac ggtgatcaat atggcctggt
     2521 tttcggcctg aagttccagc aacagttcgg ccaccttgcg tcccgcaaca gaatctaggt
     2581 tccctgtcgg ttcatcggcg aggataagga cagggcgaag gatcaatgcc cttgccaagg
     2641 cggcccgctg ccgttccccg cccgagagtt ctgatggtcg atggtgggct cgatgcgaga
     2701 ggccgactcg ctccaaaagg tattctgccc attggaggtg ttccttgctt ggtctgcctt
     2761 gtgccagcga ggggatcagg acattttcta agacggagca ctgaggaagg aggtgatgat
     2821 cttgaaacac aaatccaatt ttctgattcc tgaactttga caacgaaagt tctgacatcc
     2881 gcgtgatgtc ttctccatcc acaatgacgc gcccactcgt cggccgatcc aacccgccga
     2941 tgatattgag gagggtgctc ttgcccgagc cgctcggtcc cacaatggcc aagctgtcac
     3001 ttgatggcac tgaaaggtcg atgccccgga ggacctcgac cggttccgct gatgtcacgt
     3061 aagtcttggt aatttgttcc aaaataagag atggcatgaa acacctcagc agtgatgatg
     3121 attttgccga accacacaag ggtccaccat caagtataac ggactcggcg cgcgggcaga
     3181 ggctgagatt ggctatccgg aactggcaag ccaaaccgcg caagtccttt ggtaaggcat
     3241 cggaaagccg cctttacgtg gatacggcgg ttgccggtgt gagaatgttt ccgcattttg
     3301 ataacagatg gcgtccaaac tccccccggg caaatgggac tcggccagtt tgagcaaggc
     3361 cgactcaaaa tagaacactg gggcaaaaga accaaagcat attcgctcga tggggattac
     3421 acttgttagc cgttccaatc cggcgattcc ttccagcatg cctagatcga cataaaccca
     3481 tcctattgta acgagtttag ccagttgctg tggtggaact gcacggtggc aattgagcaa
     3541 catcagccgg agggcgggaa aatcctccag ccaacggccc aagggcgtta cctcaacggg
     3601 cttcaccggc aagagtggat tctgggcacg aacatcctcc atctcgatgg tgatttggat
     3661 gccgatttca cacttgtgcg ctaaggctag gagtcgtccc acctccggta tgtcgagggt
     3721 ataaccgtga tatcctggaa agagcctgag aacgcgcatc ttgtagtttt caacacactg
     3781 gcgaaaatcc tcggtccagt ccggcaacat gggattgatc gtcccacatg gcaccagaaa
     3841 gccatcgcca tatttgcggc attcctcgtg aagtcgcgca ttgaccgatg caatatccct
     3901 atgcagtaac gattcgagac ttcccgccca tgcgcaggta acccctgcgt tcttgaggga
     3961 cctaaccaat tccgcggtct ctgcactgtg tattagacga aacggccatt ggaaaagata
     4021 aacatgggta tcgatcagcc gataggaaga agcaacttgt ggttcactgt ccgtgacctg
     4081 agcaaaatgc agtaacgtcg gccccgccat aatgccgccc gcggacaacg aaccgagaaa
     4141 accacgacgg gttaccgagt gcggatcggt catacaggga ctcccttttt cttcagaatg
     4201 ggagccagga gtttcttgag attatctgcc aggatgagtc gtttggtgtc gtcatcaact
     4261 gaggctccca agaccttccc caactgcgtt gcgaagcttc gcccaggggc gtcgcttccg
     4321 aagatgactc gcctgggacc aagaagtcgc attgccattt cagtaaaacc cgaggtcgga
     4381 tcactaccac ttagttcggc gtagacgttg ggtgtttcgc aaaaggctcg tagcccgatc
     4441 tcccaattac ccccgccact gtgggctgca ataaagacag cgtcggggtg gcgtcgggct
     4501 aagcacgcca gatcatgagg ggtggactct cccgggagat tgcctgtggt cttaatccat
     4561 gtgtgctgta ggacaacagc gtcacagtca tgggccaaag ctacaatagg atcaagggcc
     4621 gaatcgcaac agcggcgcgc tacccagagc ttgacgccga ccatgggacc atcgcggaca
     4681 tgacgacgaa tttcgtccaa gctggccacc tgatggttag gattcacata cacaaagcca
     4741 aacgcccggg agggaaaccg ttcaatggcc cggcggacct cgttgttggc ttcctgcatt
     4801 tcgtctggtg tgggatcgta ctgccacttt gtgcccatga agacgcatac ccggccaatc
     4861 cctaagcgcg atgcacactc caagaggcgt tcgagccgct cttcgggcgt cttgccgggt
     4921 aatccaagga gatgagtgtg tccgtcccaa atctccagcg ggaatggaaa caaccgctgt
     4981 ttgatgtcgg caagccgtgg tgagaaatcc attgtcctag cgccttacta ccggaaatct
     5041 tcgtcaatca gatctcactt gaactcatcc gcggcttaaa gcccaaccca aagttccctc
     5101 tccctctagg agagggttga ggtgagggta aacccacgtt ggactcgatg gaaaaaaaca
     5161 ggagcgattc aagccagttc ccaggcccct ctctcgccga acgggatagg ggacgtgata
     5221 ggatagtctc ttagcacata ctaccaagaa ggatactcaa atcacacggc tgttcgccct
     5281 ggaaatgaca gcgccttcct ccgaactgaa aaatgatctg gtaatcctgc gatagccttg
     5341 gagaaagcgg aacccatgtc ctctcggcgg tttactcacg gcacgaccgc cggtattgta
     5401 ttgttaggtg cggtggatca caagagcttg aggtgggtca cgtccggatg agatgagcgg
     5461 aaacgtttgt cagggccttt gactgaggtt tcttgcctta gacttaggat ttacgggagc
     5521 aaaagggaga agccggaggg ggaacgacca catggatcgg caacgtcatg caggcattgg
     5581 gctgtcattt tggccaagga tattcttgat tattattgtg agtttggttg cgataggcca
     5641 gaccacgatt ggtgccgagc tcgcagagca ggatagacac acaagtctcc aatcaggtgt
     5701 tgtcaatgca ggggaggtta aacctacagc gacccggccg aatatcctgt ggctgacgct
     5761 ggaggacgtc aactgtcatt tcggctgtta tggagattcc tacgccacca caccaaatat
     5821 cgatcgtctg gccaaggagg gaatccgata cacgaatgca attgccggag ctagcgtctg
     5881 cacaccatgc cgttcgaccc taatcaccgg gatgtactca gtcacgctcg ggtcgcaaca
     5941 cctccggtcg cctattcgtt tgccgaagga ggttcgctgt tttccagaat tgttgcgtag
     6001 ggccggctat tactgtacga acaacgtcaa acaggactac aactttgaat gcccacctgg
     6061 cgtttgggac gaatccagtc cgcgggctca ctggcggaat cggccagacg gtcagccatt
     6121 ttttgccgtt tttaatacga tggtcaccca ccaaagccgg gttcgaatga gtgatgacga
     6181 gttcgccaaa gatatcgcga cattggaacc tgtaacacgg catgacccgg caaccgtacc
     6241 gttaccgccg tattaccccg acacgccggg catccgtaaa gagatggccc gatactacga
     6301 catgatcagt gccgccgaca gatgggtcgg gaagcacctg caggacttag cggatgccgg
     6361 tctgaaagaa gacaccattg tttttgtgtt cggggacaat ggggcgggca taccgcggca
     6421 taagcgtgcc ttgtacgaca ccggggtccg cgtcccgctt ctcatctggt ttccgaagaa
     6481 gtttgctcat ttggccccgg cgcagccggg cagcgttgtc tcggagatcg tgagctttgt
     6541 ggacttcgct ccgacgatcc ttcgtttagc aggagtagaa ccaccggaac tgatgcaggg
     6601 gcaagccttt ctcgggacac atgggggctc aagactccac gcctatctgt tccgcgatcg
     6661 tgttgatgaa gtcttcgagt tgagccgtgg ggtccgcgat cagcaattca aatacattcg
     6721 caacttcatg ccgcatcgtc cctatatgga gatcagtgag tattgcgaac cggcgcagat
     6781 ggttcgtgat ctccgccgcc tcgctgccga gggccagctt tcaggtcctg ccgccttgta
     6841 cttgcgagaa accaaaccga ttgaagaact ttacgatttg aagaacgacc cctacgaact
     6901 gcataatctg acggccgatc caaagtacgc ccagattctg cagcggatgc ggcaggagtt
     6961 gcgaacctac gtgttatctc tccgcgacac aggctttttg ccagagagtg atatgttccg
     7021 tcgggccggc gatcagacta tctgggaaat ggctcaggac gatgagaagt atccactggc
     7081 tcgaatctac gcggctgccg acgcggtcgg acgtccggaa aagagctacc aagattttga
     7141 agcctttttc gcagatgctg atcctgcggt tagatactgg ggggtgattg gcttaatgtc
     7201 catgacagag ctgcctcaaa gagctgtttc tcggctctac gaactcctcg acgacagttc
     7261 acctgaagtg agaatcgctt cggccgaagc cctgtgccgg cttcaaaaac cggaaaaggc
     7321 tgtccaggta ttaggcaaag aattacggtc gtcagatcct tatgttcgct tgcttgccgc
     7381 ttcaacactt gccgtcatca atggccatgc ggccggtgcc cgcgacccca ttctggaaat
     7441 cattggtgaa aaaggaaccg gcgacatgtg gatgtttacc caatgggcct tggcacgatg
     7501 ctgccgacaa ttaaactggc caattccggc gcatgttccc acccagtatt gatggtgaac
     7561 cggtatgttt ttccaaccgt gaaaagtccg agatgccggc tgggcgacta atcgctgaaa
     7621 ggagccttag taacgcgagc gacaattcct gaatactgtt actgctcgga gcggagggcg
     7681 accgagaagt agccgtagta ggtttcgttt ccacgaagga gaaagaactt gagcggaaga
     7741 aagctggcga agtcggggcg atgaatgatg tactctacgt tatccaagga gattgtttcc
     7801 cagaggtgca tgcccaccaa gacatcgccg ggacggagac cttgagctgc cgcgggactg
     7861 ccactgcgca cctcggtgat aagaagtcca ccccgatagc gcgtctgaaa tttttggcgg
     7921 aaggtttcct ccgggatcgg ttccagacgt aagccgagca tcatccagat ctcgtcgtcc
     7981 aaagtcgcgg tcttgggttt ggacagtgtg agcataagtt gctggttgcc ctcgccattt
     8041 cgcgtcacta cgaccggaag cacgtcgtcg gcagactttt ctagcaaggc tcgttgaaag
     8101 tccagggcgt tccggacctt gcggttggcg acttcggtga tgacatcccc gggttgaagt
     8161 cctgcggcgg ctgccgggct gttgggatcg acttcagaca cggtcaatgg gccagttggt
     8221 ggagcggagc ggaaccgcac accgtagtag agcttttgat cgttgatttg ggcaaggagc
     8281 tcggcagcgg cctcgacaac ccgatccacg ggaatggcaa aaccaattcc ctgcgccccg
     8341 gcacgaacgg cgacgttgac cccgatcatc tcgccgttga tgttaagcag gggtccaccc
     8401 gagttgccgg gattgatgct tgcgtccgtt tggatcaagt cgtcatagta ctgagagtca
     8461 ctgacttgca ctgcccggtg aagagcgcta atgatcccac gtgtggcggt atgctcatag
     8521 ccataggcgt ttcccagggc aattacggac tcgccaggca tgaggtcggc agacgtcccg
     8581 atttggatga cttgcagagg ctgatcggga ttgatcttga tgatggcaag atcggttttg
     8641 gggtcccggg cgacaagttt gccgatgtac cgcgttccgt catgaagcgt tacctcgatt
     8701 tctcgaacac cgtccacgac atggtggttc gtgatgatat aaccacgctc gtcgatgacc
     8761 acgccggttc ccataccgtt gacacggcgg cctgcatcgg gccgtgtgga attgccagcg
     8821 gtagtgatga gcgatttctc accgcggatg ttcaccactg cgggacgggc acttcggatg
     8881 gcccgaacga taggggtctc ccgcaggtct tcattaagac cgaatgttgc ggagagcccc
     8941 gttgcccaaa ctaaaacaca gagaaccgag atagaccagg ccggaaatcg caggcagcat
     9001 cttgtgagca tgttctacgg caggccgttg ctaaagggtc gtggaaaacc gtgccaacag
     9061 tgtgactttc cttcttcaag tgtgggaaga aggctcaaaa gtgtgcatcg gcgagagaat
     9121 cagcatcctt gatacacgtg ctcgtaaccc acaaagtctc taagctttgt ttcgtttgcc
     9181 cacattgcca tcggttaact catgcaatga gtgcttgcct gggaacacaa agtggaaact
     9241 gctcgttccg cacgatcgac gtgctctaag aatgcccgag tagaacgggc agccccaacg
     9301 gaggatgcta ggacttacct ttgcatcccg gttgttcggc gggctgacgt ggcggtgggg
     9361 ggctggcttg ggagaatcac acgaggttct tcccccgtat gcgcgatggc gctagaaaaa
     9421 ggcccctgaa acagccagga aacctggtgg cttggagcaa acgacacgtc cgacacattg
     9481 ggagaaggct cgtcatccct accggctttg tcactggcag tgctggatcg ccaacggctg
     9541 gactcataca tccacatccg gggtgtaaca gttcgtgttt gatcgttgaa ctccccgggc
     9601 ggcagaggcg gcggcgtagg aatcacttcg ggtacggagg gggaagaatt taggtccgtc
     9661 tctttagctg atgtctcgcg ggactgctcc cctatggcat tgacgcgaaa aacaggccgg
     9721 gtgggaaggg gaaagaactt tgcatgcgat gacccgatgg aggcggtgaa ggattctgga
     9781 aaaccctctg gtcctaagtc cgcatcgggc gggggagtta ccggaaaacc gtttccagag
     9841 cgaaaacgtg gcgatccccg gcaggtttcc gacgcaccca ggcagggtga acaaggccgg
     9901 cagctagcgg tgcgagagcg ataacacttg cagcccgcca ggagtatcaa acaaatcaga
     9961 agcgaccgag agcaaaaatg agccacctga gtgaatcggt cttgtccaat cgagaaacac
    10021 aagacaacac aacgatttcg gacaccctca ggtgtcatcg tcttgcgtcc tgtcaaacgt
    10081 tgaggaatat tccggttaca aggcttgcac gaaagaagag gatacttccg actggttagt
    10141 atgggcgatt taaagaagag cggttgagag ttccaaggca cttagggatt gaaaaggctg
    10201 acagtagccc gaatgtagtg ccttcctgct tgctatttgg tttacgctgc ctttggtaaa
    10261 ctgcgtggga tgggaacgat gggacgtata agcgattcgc gagccctaat gccgtcgaga
    10321 atcctttccg ccagttgcac ggcggctagg ccgtctccgc ccgtaactag gggaagggtt
    10381 cccgttcgca cggcgtcaat aaagttgcgc aattcggcct caaggggatc gtaagacgcg
    10441 atttcaagct gtcgccatgg gaagaatcgt gacgtaaatg tctctttgag actattcagc
    10501 tcttgtgggc taagttcctc gatacggaag gcgccagacc ggatctcttc cggatattcg
    10561 cacaccgaag ccgttcgtcg cgcaaagtcc acgatcgctt gccctgccct gtgccagata
    10621 cgcatagtgc ggattggttc ttgctcgatc cgcgaggcct tcaggagggc aaccgggcca
    10681 tcttcgaatt cgattcgggc tatggccagg tcctcatgtc tgcccaagag ggacacaccc
    10741 acggagtcgg tccgcagaac gggcttcctg accatcgcca gaatcaggtc gagatcatgg
    10801 atcatcagat ccatcacaac gcccacatcg gtaactcgga aactgaaggg accgcaccgg
    10861 gaggcttcga tcagttgaat gtcacccaag agtggctgca ccgcccggta ggccggattg
    10921 aaccgctcga tatgcccaac ttgcaaaaca agattctttc gttgggctgt gctccacagt
    10981 tgatgggcct gttggggtgt ctcggtgatc ggtttttcca caaacacgtg gacccctgcc
    11041 tcaaggcaat ccattgctgt gcggtggtga agagatgtgg gcaccgccac cacgacggca
    11101 gcacagtggg gtaagagatc acgataatcg gcgaaggcgg aacaccggca ttctttagcc
    11161 acggcctcgc gacgatcgac gagcggatcg gctacgccaa ccagcctgac gtcgcgaagc
    11221 cttgcgagct ttcgtgcgtg aaacgtgccg aggcgtccgg ccccgatcac gcccacgggg
    11281 atgggattgc catttcctcc agatgatgct gacaaccaat ctgtgctttt catcctgttt
    11341 tgtaggtgca gttatgatgt ggggttaggc agccttctag acctcaggca caggatcgaa
    11401 tccgctccac gagaggaaac tggttgagga gattccggcc catctcggca tttagccggt
    11461 gtccacttcg gtacgctgaa aaacgcccca caaagtcgca gccgcaaagg gccaaatccc
    11521 caaccatatc caaaagtttg tgtcgtacac actcatcggg aaatcgaagc tgattgtcga
    11581 tggggccatc ggcgtcgaac accaacacat ctcgtggtgt cgttcgtcgt cctaggcctt
    11641 gtgagagaag ccactcggcc tcctctttaa gcatgaacgt ccggcaggga gcgagttctc
    11701 gacaaaatga ctcgggcgtc aaggctaggc cgaagctctg acggccaatg gggccatcag
    11761 ggccgtaatc gagcaggtat cgcatgaaac actcattagc gatgccgggg cgggcctcga
    11821 accagctatt tcgatcctcg accctgacaa ctcgggtcag gacacgcacc gcccgcgggg
    11881 ctgattgctt gatgatccca gcctgaagca gtgcctccac gaacggtagg gccgagccat
    11941 cacagcctgg catttcggcg gccgtgaccg aaatttcaca attgtcgatt tgtagtccgc
    12001 cgagcgcggc catcacgtgt tcgaccattt cgaccctggc accactaaac tccagggaac
    12061 tgcggcgggg cgtttccata cgatattcga gccgtgctgg gatgcgtggg cgatgtcgaa
    12121 ggtcctcccg tacgaagaca atccccgaat tgggaggggc tggccggaat tccacacgga
    12181 cgtccgttcc ggaccaatat ccgaaacctt caacggcaac agcacgtgcg atggtttgtt
    12241 ggggtctcag agaaagcatt aacgtctggc gtctgtcgat cgaagatgtt ttaaggacgt
    12301 cacataggtc aacaataccc tgccgatttt gcctaacctg gcggccgttt ggtagccccc
    12361 aggttaggca catctctcgt acaagctaaa atttctcaat cgtggaacgc gcggggcgaa
    12421 agatcaaaga aacgcctttt ttgcacctcc gcacaccgca gttcaagttc gctcagcgga
    12481 gcgttccggg acgaggaaag ttcccctgtc cgggaacaac tggcgacgat gtgcttgacg
    12541 ccgacgatgt ggtcgattgt gcccttgcgt tcagtcgttg cagcacgagg ggcgtcaaat
    12601 ccagttccgg ggcctggtaa acaatcagtt gattgatgta ccgcaagatg tcatcaggtg
    12661 aattggggtc aacctgctga ccattgaatc ggagaaccac ggcgattccg ttggcagcag
    12721 ccaaatattg aatctcgttg acaatctctt ggtaggtggc gtagtggagt ttcgcctctc
    12781 gcaagagaaa ctctttacgt ttcttgatga tctcagcctc aaagtctttt tggcgcttga
    12841 caagcgtatc atcgatttgg ttataggttg cagtccccgg ttgatgatcc ttgagttgtt
    12901 ctgccaacga cttgatggcc tcctgttcgc gctgtgccca ctgttcgacc gccgtcgcct
    12961 cagaccgcag gacactcatc tcctgtttaa atcgcaggtg gttcttgaaa atgtactcca
    13021 catcgaccac ggcaatgttt cggacaagcg ttgctgaccg cacaggtgcc gttggagttg
    13081 ttacctgttg ggcacgcaac tgataaacga tcacgcccgc aacgacaagc cctgctgctg
    13141 ccaatgccaa ccatttcttc tgcaacatcg aaatcctcct tgatcgtggt gaatggtcgc
    13201 tcctgaccaa ttttccaaag cgtatcgaat tcctgactct gcgtcgagat caaattgggc
    13261 aacgaccgat ggcatgtgac aaaaaggttt accggatagt gtcattaggt accacccgtt
    13321 atggtgggct cgaccctcca gcgtttttga tgcctgcctt cccttagctt tctcgaagaa
    13381 aacagggtgg cgagccgtct cacgttaact cacttccgac agacgccatt cttccaccga
    13441 actgggctca aacggcagcc cattgtgggg attctaggaa cattcgtaaa gggcaattcc
    13501 gtttagcacg atgcaggcaa agtgtaaccc cagaagcaga gtgccacctg gcgggaaatg
    13561 accggcttgg tccattcatg gtaagctaag ggtttgcggt tttacttcca acgacagaat
    13621 tcgaattggc attattaagg ttggcaaacg tggaattgac tcgataccaa gttacggttt
    13681 tacccctggg gcggacgatc gaggttctgg ccgggtcacg atttgtggat attgcccgca
    13741 cgttgggatt ggccattagt ctgccctgtg gcggagaggg tgtgtgcggc aaatgccggg
    13801 tctgggtgcg gtccggaatg gcggagccga cagctgccga ccggcgtcat ttgacggagt
    13861 cggagcttga gcaaggacag cgcctggcct gtcaattggc tgtgcgatca gacgtggtta
    13921 ttgagatccc cgagacgctg cttttgcggg catcacacca gattttgacc aaaacaggcg
    13981 aggtccgatt ggaaaaggac accccactgg tgacaaaacg gacggtcaac ctaaccgaga
    14041 ccccgtcggc aaccgatttg gtggcgatcg ttcgtcatgc cttgccccag gtggggttac
    14101 cgctctgcgt gttgcaggac ctgtctcagc aactattgga aattccgtca acgcggttag
    14161 ccgtgcttgc ggagggttgt cttttagcgc tggaaagccc cgaggaggct tctccgacct
    14221 taggtgtggc tgttgacttg gggacaacca ccttggccgc ttcccttttg gatatgacaa
    14281 ctggagcgga actggccgta tgtgggcgac taaatccaca gacggaattt ggggatgacg
    14341 tcatttctcg gatccaattt gccagtgaga caccttgggg tactcaacgc cttcaacatg
    14401 ccgtggtttc tgccatcaat gagatgattg aagagctgat tggccaggcc ggggtcgatc
    14461 gacaaatgat ttatcaagtc gtttgtgccg gcaacactac catgcagcac cttttttgtg
    14521 gactatctcc ccagcatctg gggcgatttc cctttcgggc aaccacgttg gaagctcaac
    14581 gtttctttgc cagcgaggtc gggttgaatg ttcatccccg tggccgggtt tatgtgtttc
    14641 cagccatcgg cggatttgtc ggaggagata ctgttgccgg gattgtgacc accggcttgg
    14701 accagagtcg ctcccctgcc ctacttgtcg atctcggcac gaatggtgaa atcgtcctct
    14761 gggatgggca agttttgcgt gcggcagcca cggccgccgg accggcattc gagggggccc
    14821 ggatcaagtg tggcatgcgg gcgacagagg gcgccatcga aagggtgtgg tgggacgaaa
    14881 ccgcccaaca gcttcgttat catgtcatcg gcggaacaga tcccgtgggc ctttgtggtt
    14941 ctgcaattct cgatgtcctg gcgctcttgc gtcaatgggg aattgttaca gcagaaggcc
    15001 gattgcaagg gggcgcggca cttaggcgga cacttcccga agacttggcc catcgactca
    15061 tcgatgatca gggtacacca gcgtttataa tagttcagaa ttccagaggc gacgcgcgcg
    15121 gcgatatcgt cttgactcag cgtgatatca ggcagttgca gcttgccatc ggggccatcc
    15181 gtgccggaac cgaaatgctc ctacggtcag cagagatagc accggaggac ctgcagatag
    15241 tttacctggc gggcggtttt gggaattata tccgtcgccg aaacgcgcag ttggtcggtc
    15301 tgttgccacg ccacatccct acggaacgga ttcaattttg tggcaacgct tcccttttcg
    15361 gcgcgaaaat ggctctcctg tcgcgggagg tctgccggcg ggcagaggac gtttcgcgga
    15421 aggtggaaca catcgagtta gcgtctcacc ccgacttcca ctggaagttt gcagaagcca
    15481 tgatctttcc cgactgccag ccactataag agcgcataac tcagcgcttg ccagcgagga
    15541 caacgcccgt gcgtaccgaa aaaatctttt ctcaatgacc cccgcaacca gggcaaccgc
    15601 ccccccgggg gtaattgggg ttattgatga caaatccccg ccccgctggc ccatcaagaa
    15661 aatcaatcac tgtgccgtcc aagtgaaggg caaacttctt gcgcgtaacc aacgaaacgc
    15721 cgttcgattc aaagcgggcg tccagtttcg ggtcgtattc cgtatcaaat cccaaggagt
    15781 actgcagacc cgagcagcca ccaccgccaa tggccatgcg taggacagtc cccggttcca
    15841 gtttttgctc ttcaataatt cgtttgattt ctgacgccgc gactggggtt aagcttaatc
    15901 ccatagactc gtgatcctcc aacactaaat tctttctaac agacgtttcg agcacgccga
    15961 gttgaccggt tttggaattg ttccaatctt tgatgaatca tacgcaaatc gtggcgattt
    16021 tccaaggatg tgatccacaa aggggagagc acgagtggcg atggcttggc agcggagagg
    16081 tctagtctct tggaaggcgg ggatgctggg tttccaacct acgattgctg ccccgctaaa
    16141 accgtgtgat ctccaatgac cggcgaaccc ggcaaaatgg cgatcccctg tttcccctgg
    16201 tggtttcttc gcggtaagct taaacaaaga gaaccggttg acggtattcg acggctgatt
    16261 aacgtcgagt ggggacgcgg tttatttttg gttctctcct cgaatgttcg tgcgcgcacc
    16321 tgttgcgtgc tttgagtttt tggagtcgat ctcatggaac tccagcttat cgattacttc
    16381 cggcagagaa taccccaata cccctgtgtc cgcttgggga ttggcgacga tgctgcgatt
    16441 attgacctcg atatacccca atgcgttgtg accacggaca tcctgatgga tggcgtggat
    16501 ttccggctga gcgaggtaac cccggaggcc attgggcgga aggcccttgc ggtcaacctg
    16561 agtgacctgg cggccatggc ggcctcgccc aaggcgtgtg tcgtaggact tgtgcttccc
    16621 gagaaaggtg gggtcgagat cgccaagcgg ctctatgatg gtttgttgcc cctggcccgt
    16681 gattttcaaa ttgctgttgc tgggggagac accaatagtt gggaggggcc actggtcatt
    16741 agtgttacgg ctatcggttt accctctccc ggcggggggc tttgccgtgc cggtgcgaag
    16801 cccggggatc ggattatcgt caccggtgac tttggcggat cccttctggg caaacacctc
    16861 acattcacgc ctcgggtttg ggaggccatt tatctctacc gttcctatga aatacacgca
    16921 ggaatcgacg tgagtgacgg attggcgctg gatctctggc gactgaccgt ggaaagccgc
    16981 tgtggtgccg tcattcaggc ggagaaggtt cccatttcgg aggcttctca tcgcatggtg
    17041 gcccaggtca agtcccgaat gaagcctttg gatcatgccc tccatgatgg tgaagatttt
    17101 gagctgatct tggccgtgcc tccggccgag gcggctcgga tgctccgcga tcaaccgttg
    17161 tcagtcccgt tgaccgacat tggcgaattt gtcccggatc gaggtctttg gctggaaaag
    17221 ggaaaaaaac ggaccccact ggaaccgaag ggataccaac acaagttttc cctgtagaaa
    17281 agagagcttt gaacggcatg cccaaagtac gggtcgtctt ctatcgactg aaacgttttc
    17341 gggtcttagc cttttattgg gcgtatgact gatcggttag cgtatggcgt cgcgcccgta
    17401 cgatggaggg ctcgctggtg cgcaggagtg caaatgaagt tctttcctta ttggtgaggt
    17461 gaaaccgagc cgtggatgcg cgtggaaacg tgataacacg ttctgaagaa gaaacacgac
    17521 agctgggagc gttgattgcc caatgtttgc cccggccgtc tgtgatcggg cttatgggga
    17581 cgcttggctc aggcaagacc cggctggtcc aggggattgc ccacggctgc ggagtccgcg
    17641 aagcggcggt gatcagtcca acgtttgtct tgatccacga atatccgggt gacgttcccg
    17701 tcttccattt tgacgtgtac cgcctgaaag atgtcgcgga gtttctcgac cttgggccgg
    17761 aagagtactt tcaacgtccg ggaatttctg tcatcgaatg ggcggaccgt gtcgaagaat
    17821 atctcccgcc gcatcgcttg attgtggaaa tcgaagtggt ttcagaatcg gatcggtgct
    17881 tcttgttccg tcctcagggg caagaatacg aaaaagctgt tgatcagatc atcgagatgt
    17941 ggcgaagagc ggcaggtgat ccgatgagaa aattggtcgg caaagactgt ccaaattcgc
    18001 gggaagaggg ttaaccagtt cgttttcgct tttccgagga gggtataaaa tcttcttggg
    18061 agaaaaaaag ttgcagagac ccagcgcggc aatctatact cgaaagtagg cctgaacccg
    18121 ttgcaaggat gtggttgtcc gatcacacgc tgcttgtttg tgtctgcctt tgagatttag
    18181 gagggctggt ggtgtcccgt cccgacgatg aagcctttaa cttagacgcc attttagggg
    18241 caggcgcccc tgaggtagaa ggcatgccgg gcgccccgcc ggtaagcccg gatttggctc
    18301 cacccgcggc cgaggctcct tcggaggctc ccgcggaggc caagccgccc aaaccatcgt
    18361 tgtttgcgcg attgcgcaga atcgacattt ttacggttat gctcggcctg tcgctgttgg
    18421 ccatcatcat tgccgtggtc atgctggcaa ttgagttgag ccggtatcaa tgggatacct
    18481 ccgcccggtc ggcccgccag acagttcgat gggtaacggt gacttctgag caccttgtct
    18541 aggtgaagcg aatacttgtt gattgtctgg gactaattgg aatctgctgc ctgctaggtc
    18601 cgcttcgcat gcttcctgtg atcgccctac cagcttggat gggccagctt ccccctatcg
    18661 ttgaggcgac ggtgtcggac ctatcatgcg ccgtttgtca gacctacgcc acttccacaa
    18721 ccagggcggc tgcctgaccg gccaaggtcc aattgacagc caccagaaaa ggcgaagcga
    18781 gcggctgcgg ggcgcctcga acgacgttga tagggcatct cggatcagct tcatcatagt
    18841 gcaaggtagg cgggacaagc cggtctttgc cgagaacgag gcttgcagcc aattcaacgg
    18901 caccacctgc cgctccagtg ttaccaaagt aactcttggg cgcggtgacc gggacatcgg
    18961 agaggaccct gcgaatccca tggctttccc ggatatcatc ttcctcggtc cccaatccgt
    19021 gagcgttcac ctgagccacg tccttggatt caatggcagc atcttgtaaa gccagggaga
    19081 tggcccggga aagtgaggga aaggaattca agtcaccgtc gagtggtgcg taacaacgtc
    19141 cccagccgcg aattgttccc caaatggtgg ccccgcgtgc ctttgcatga tttacgcgtt
    19201 cgaggacgag aaccgccgcc gattcccccc gcacctcacc gtcgcggtgt ttgtcaaaag
    19261 gtcgtggact cgcccggccg ttgtccacag cccgcgaaaa ccgccccaga ctgacgaatc
    19321 tcgcccaatc aaatgggttc atttgggagc tcgcaccacc cacgaggacc acgtccgcta
    19381 gcccgcgttc aagtaatgag gcgccttcga tgatggccag caatgccgac agatcgccgt
    19441 gatgaatcgt gttattggga cctcgcgcat caatcgcaat tgagatgtgc gaggcaatca
    19501 tgttgggaag gacctttaga aacactaacg gaaaggttgc aggcatgcca agcggtgccc
    19561 agcgatcgaa gtgaaattga ccatccacca ggcatgcctc ataggtcggc ttgctgtcct
    19621 cgagggctcc acatattcgg tcggccccga ggactaccgc gatacgctcc ggatccacgc
    19681 cggctgaatc caacctcgcg tccttgtatg ccaaaatgga ggctgccaca cccatttggg
    19741 agtctcgcgc catcactttg acacttttgc ggttggggat gaactgtcgc ggatcgaagt
    19801 ctcggacttc ggcagcggaa tggacaggca agccggacga atcaaatagc ccaatcggtc
    19861 ccaccccgga tttccggtgc gaaagtgact gcgtaaattc ctcccgtccg ataccaatcg
    19921 gactaacgac cccgatgccg gtgataacaa tttcagaatt taacattgtc aaaaatcaac
    19981 aggaatcgtt ttgggaatcc tcaatcacga ttgtccctac ccactacatc ggcaccaccg
    20041 gcccacactc aaagtcacaa gttggtgcgc aaatgacgtc actatgcgac caccgcaaat
    20101 gcgtttcggt aggttaccct cggaacccct catttttagg ccaaaacctt nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaagtttgcc aattattacg gtcgcggcat
    20281 ttgtgaggcc gataacagtt gtaggttctg caatgtgacg tactccacct gaagtaggag
    20341 gggaacgatg cgaacaagca aggcattcat actgctctct gccatctttg gactggcgac
    20401 cattattggt tgtcggatgt gtgccagtcc ccacgattcc tgcggacccg tggtctcggc
    20461 gggatcctct ggccggggtt gtacctcagc ccgggcggga tccgccttcg ctgttacggc
    20521 ttactcggat catacggacg aaaacagccc gggatatgct tctgccccgc acgtcgccgg
    20581 gcttgattcc gatcgcagaa ccgtatcttc ttcgaaaggt cccctaccca gatctccgca
    20641 tcgcccgtta accccggagg atatcgatcc gtttgttaaa ctcgggattc ctcccgagaa
    20701 tattctttct gtcacagatc gaccggcgga tgagcctatc gcccaagaga cgagtccttc
    20761 accgcaaacg ttggtgcacg ccgatcccaa gcagcccaaa gcacctgcca cgaacgcaga
    20821 ccgagagagt gtcaccagtg caggctggac tcgcattggc gagcggacgt cgggcacact
    20881 tcgctgaaaa cagagtgttt ctcgagcagc aagaccaggc ggtgtgccca gggcaaggtc
    20941 cgtcagtcat agcgtattgt ttctttgcga cagcttgcga cgccgcgcat gatccgcatc
    21001 ttctcgccga tctcttctcg acaagccaag gaatttgcgt taggccgacg cgccgcctaa
    21061 aatgacctca tcgtctctcg ggtggtaaca aaaggaagcc gcggctcgtc cggcggcttt
    21121 tcttcgacgc cggtgtggta ccacggaaat ctcaggcgca accgacgcat gcatcgccat
    21181 caggggagcg aaggcgggct atctgcgaaa tcgcctggat ccgcacactg gccgggtttt
    21241 ggaaagggtg gtccatcttg ccgaggagaa acgaccgtga gcgactccaa tcgtccagat
    21301 gtgaagacaa gcggcgtgac catggcagag gccccagtca ggctaggaga aatgctcctg
    21361 cgaatgggcc ccggtatgat cattgccgct gcggtggttg ggtccggcga gctgattttg
    21421 actccgcgca ctggcgcaga agcaggcttt acgctcctgt ggatcattct gcttggttgt
    21481 gtcaccaagg tatttgttca ggtggaattg gggcgttacg ccatcgaaag cggccagacc
    21541 actttggcag cgatcaaccg tctgcctggg ccacgctggc gggtccattg gatgctttgg
    21601 tattgggtgg ttatgtttgt ggtgagcctt gctcagcttg gtgggatcgc ccatggagtt
    21661 ggccaggcct tggctatagc acttcccatt gatggaagct ttcgacagct tctcaaagag
    21721 caggaggaat gggaccgaga ggctgcacaa attcgacggg agttagaagc atcgtttgaa
    21781 caaccgaagg ggtctggatc ggcaggcacg gcgagcgttg acaaaaaaac cgttgaaaca
    21841 gaggtggcgc agcgcatgca acggccccgt ccgggttacg aaaaccgaga gggcttactg
    21901 acggatgacc taatctgggc catcatcatc tgcttggtga cgattccaat tctcgtctat
    21961 ggtcgctatg ggatgatcca atgggtgtgc acaatcctcg tggtcggttt cacgctggtc
    22021 acgatattct gtgtgatcgg tatccagttc aagcctgagt gggccatctc ttgggatgat
    22081 ttgcgggagg ggctttcatt tcggcttccc ggaactgcca atcgttggct agcactttct
    22141 acagccttgg ccacattcgg aattatcggc atggggacca acgaattgat tacctacccc
    22201 tattggtgcc tagaaaaagg ctacgcccgc ttcacgggac cgcgcgataa ctcaccgggt
    22261 tgggcaaaac gagcgcgggg atggatgcgg gtgatgcggg ccgatgcgtt tctgtcgatg
    22321 gtggtttaca cgttcgtgac ggcagccttc tatcttctcg gggccgctgt cctataccgt
    22381 tcggagctga ttccagcggg cagtcagatg atctcttacc tcagcgaaat gtacgtcccg
    22441 gtgctcggtg agtggacaaa ggccttcttt ttgtttggcg cgttcgccgt gttgtattcc
    22501 acgtttttct cggcaacggc tggccacacc cgtgtggcgg cggacgctgt cgccattgtg
    22561 cgacgtggaa ggtctcagcc ggatcgaccg cctcaaagct gggtgaccgc ctttagcatt
    22621 ggctttccgt tattcagttt ggtgacgtgc cttttctatc gggaacccgt gacactcatc
    22681 gttgccagcg gcttcatgca agcgatcatg ttgcccatgc tggccggggc ggccttgtat
    22741 taccgctatc gagaatgcca cgaagcattg cgcccaggac ttatctggga tatttgcctt
    22801 tggatttcgg cgatcgccct cctggttgcg ggacttttcg gggccggaat gggcttacac
    22861 cgcctgttgg cacattaggc cgtcaacttc cagtagtctc cccaccccag aacgcgccgt
    22921 acctcgagcg gggtcgtgcg cccttctttc accgcagtta tggcattctc ccaaagagtc
    22981 accatccccc gttccatcgc tacccgctcc aaggtgatga catctgaacg atctaacacg
    23041 gctcggccaa ttgcctcatc ttggaggaca aggacctcag cgatgggaaa acgtccaacg
    23101 tatcccgtgt gacgacactc ctcgcaacct tttgctactc gcacctggtc cacaggtaac
    23161 cccagcatct gacctgaatc acctgtccat tccgcgcact cgcatacttg tcgcaagagt
    23221 cgttgcgaaa ccacgactag cacaccactg cggatcaggt atggctcaat gcccatatcg
    23281 agaagccgtc caattgcctc ggcagctccg cccgcgtgat aggtgctcag gatcaagtga
    23341 ccggtcaaag aggcttccac ggccacttcc gccgttgttc gatctcgaat ttccccaacc
    23401 gccacgacct cggggtcctg cctcatcaag taccgcaaag cggcagccaa atccaagcct
    23461 acccgtgggt caacctggct ttggatgaca ccgggaattg ccacctcaat aggatcttca
    23521 accgtcatta ggttccgcgg gatcgtctct tcggtgagtt cccgcaaaca ggcatacatg
    23581 gtggttgtct tgccacaccc agcgggtccg gtaataaaga tagcaccgga tgtttccgtc
    23641 aaagccctgc gaagaatctg aacgatcccc tccggaaaac caaggtcatt aaggacttta
    23701 aaacgggtct cttcgggaaa gaaacgcgtc acaatacgtt ccccgtaaag ggaaggaaac
    23761 gtgctcacgc gaatctcagg tcctacgcct ggtagcctga tccggccttc ctggggaatg
    23821 tcggttctgt acgtcaacag gtctgccaaa accttgatcc gggtgacaat ttttctctga
    23881 gcgaagttgg gcaggctgcc cgcacgatgg aggacgccat ccaagcggat ccggacttcg
    23941 agacagtcag ccatgggatt gaggtgcagg tcgctggccc caagactgct ggcccagttg
    24001 atcagttgtt ccacaacgtg aacggcaaaa tccggatcat cgtctcgaca aagtgctgca
    24061 atttcggaaa actgtgccag tctggcttcg ctcatggcgt tgcgtcccct ggtttaggcc
    24121 ggtgtgttca cagagtttcc acgccctggc ggacagatta aaatcaagtt aagtcctatt
    24181 tgatcataac cgacatgaag gttgaaaccg agagggtggc tagggcggca aggaaagccg
    24241 gggtagcatc ttcctaactt cgtaggcgag gaacgaagcg ctcttggatt tccgggggat
    24301 cgtcatcaaa gcacgctggg tctaggggaa cagattatgt cggaagctga aaaaccgcgg
    24361 ccagagaatc tgcataatcg atgtgagggt gaaactgatc gaaatgccat cgattccgag
    24421 aacgtcatgg aatcggctgc agacagagat tccgacggtt ccgggaccga gatcctcaaa
    24481 aatgagctgg ttccaatgga tcacgatgag gagaagagat ctgctttttt gacaggtgag
    24541 gaaattctag ctccggaggc tgttgagaag gttgaggtcg cggcggcaga gtcatcagat
    24601 gccccagaaa atgacagcgg cctagagttg cccgaagatg agctcctcga tctgccgcgt
    24661 tcgacggatg cccgtgttat ctcttgtaca gacacagacg tgtcttcggc agagagcgcg
    24721 gaccaaacag gggaattagc acagcaagaa gcgttggagg tcgagacatc agctgtctcc
    24781 gagccggctg tcacagaaat gactgccgag gaacagccag ccgcggaagg cccttctgat
    24841 caggagggca ccggcgccac cgaggtggta acgacagagc ccgaagcgga atcaacgatt
    24901 ttgatggtga gagtaccagg agacgagcgg tggtttccct taagcgaagt cattgttcaa
    24961 actacgggtg aaccctttgg tgaatccgcg gctcgctgga tcagtcggct ctatcggcaa
    25021 tcattggaat cgacacctga gggaccggag atgtcatcgg ggccgtcaac cgctgtgggt
    25081 gaggtggtct ttcccagtat caccacggcc cccgaaccaa cggattaccg ggaacgactg
    25141 aagaaggctg cgccgaaaaa atcgatcgtt aaagagttgc tagggatcgt gctggggggg
    25201 atcggagggc tccttattgc gtattacgcg cttaactggt tcggtggacc gcgttacgat
    25261 tttgcgaaga tacccctacc gggacttcac cacacgtaca aacatgcacc gccgtggctt
    25321 aaagatttgc tcgaaggcaa gtattggacg acgagcggtg aggatagtag tgcggatgaa
    25381 caagtggaac cgtgacggac gtttcaacga agacggtcta tttgcggtaa tggccgtttg
    25441 actcaatttg ggcgattttt tgcaaacgtc gagcgtggcg gccgccctca aattctgttt
    25501 gcagccatac gtcaatcaag cgaatggcca ggagttcgcc aaccaggtcc gctgcaagac
    25561 ataatacgtt gagatcattg tgccggcgag cgatctcaac catcaactcg tcatgacacg
    25621 gtgccgcccg taccccaggg aacttattgg ccacgatcgc cattccaaga ccagttccgc
    25681 agatgagaag gcccctttct ccttcgctgc cactaattcg ccgagcaact tcggcggcca
    25741 cgtcggggta atccacccca ttctggctat ccacaccgac gtcgataacc tcctgcccga
    25801 ggcgactgag ataggcaatc accttgtgtc gcaaggtgga acctcgatgg tcgctcccga
    25861 cgacgattcg catggtctgc ctatctcccg acgcttatgt tacacttgtg tcctcgcccc
    25921 attctgccgc tcttggcgag cgaaactcca ccaaacggtt cgccggaacc atttgtcctg
    25981 tttttcctca cgatcaattc tagaccccag cttaaccttc cactatattc tcaattccgt
    26041 catcttccaa gaatggt
//
LOCUS       DAOSUT010000047        25964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1233_length_25964_cov_9.282141, whole genome shotgun sequence.
ACCESSION   DAOSUT010000047 DAOSUT010000000
VERSION     DAOSUT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 25964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25964
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_25964_cov_9.282141"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLD05_06015"
     CDS             <1..234
                     /locus_tag="PLD05_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77027.1"
                     /translation="RKSPGKRAVLLHPTVGFSKYLLHCAGAVVAGNRLVEATAHPSRG
                     LCREGRRPRRPLHLIWTGQVKLNHGEIFRSERF"
     gene            545..2389
                     /locus_tag="PLD05_06020"
     CDS             545..2389
                     /locus_tag="PLD05_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77028.1"
                     /translation="MTMSVRIYLLVTILLIIGRSPNIVALERPAEPVYEVVSPLGERV
                     VEAGAMAPRLETLNGKTICMVWNHAFKADVTLPAIAEELKKRFPDIKIVPYYSMPLAP
                     LPELPGSPTKESENLRAALKAMGADAVIVGNGGCGICTPRACYATVVAEQLGIPAVVV
                     TNPGFDEQARTMAQYSGIPSLQVAVYPDPFDLETEAQLVEKSKTILVPQIIDALTKSI
                     AAAKDSVKIDSRKIVFTGTLDEINRYFTALGWSDGLSISPPTVDRIEEFLKYTDYGPH
                     EEIAVLPPANLKATPWTIAANGVMAGCRPEYMPILIAAVQAMGDPAFRYRSHGASTHS
                     ITSFFWVNGPLARQLGIDHGQGLIAHPVNAAIGRAMSLIERNIAGLRIKETQMGSFGK
                     MMSWVLAEDEQAMIQWGWEPYHVEKGFDRNANTITAGTSTVWGRNLVPATSEPEIITQ
                     LIAYDAVNTEAFASGFTGQSRRYVLIGPGVAEILAGGGYTKGTLRRDIMRMGRKITYE
                     AVFSQVYGSFGRVQRSFEEELRRALRYSEKGKLPPWYPRFPGWEEIETVPAVGMIEFI
                     VCGDPIRNKVQILAGGPGIASKEIKLPANWDELMQKAGYRPLREFYLQ"
     gene            complement(2733..>2963)
                     /locus_tag="PLD05_06025"
     CDS             complement(2733..>2963)
                     /locus_tag="PLD05_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77029.1"
                     /translation="GTTMTHAKLVPKDAGDRVPPRLATDRTNKRDHTADATEREGDYG
                     GQLRETVPDRFAESKERDAIPNGPRTPLDRRR"
     assembly_gap    2964..3063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3547..6363
                     /locus_tag="PLD05_06030"
     CDS             3547..6363
                     /locus_tag="PLD05_06030"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOP77030.1"
                     /translation="MSRSLKRCVGVLFLFSLGWVGHNLLAEQAGISPWSKNPYYWQYN
                     GKPVMLIGGSKDDNLFQIPDLKEHLDEMVAVGANYIRNTMSDRPDKGFEVYPFKKLEN
                     GKYDLNQWNDEYWQRFENLLKWTKERNIFVQIEVWDRFDYSRDNWEPHPYNPKNNINY
                     TYEESGFAEHYPDHPGSNKQPFFFTTPYQRNNKVVFPYQERFVDKMLSYSLQYDHVLY
                     CIDNETSGEEEWAKYWAEHIRRKAAQIGKKVCVTEMWDDWDLKAPRHRRTLDHPELYA
                     FADISQNNHQKGQTHWDNFQWVRDYVKNQPRPLNSVKIYGADTGRYGNDQDGVERFWR
                     LLLGGAAAVRFHRPDSGLGLNDKAKASIQAARKVESLVPWWELRPAMELLGNREANEA
                     YAAAKPGEACVVFFCNGGEVTVNLSQRAAGEAKLRWINVSDGQWGPEGTTKAGGLVTL
                     KTPQAGPWVAVILRNSPAVAARQSGSSDVKRSVPATLISWAEKAPQKQTAHGVLRVCP
                     ANPRYFTDDSGKAILMVGSHTWNNLVDMGPGDPPPAFDFPAYLDFLEKYNHNFIRMWT
                     WELLTWDTAGNREPKAQVHVAFPHPWKRTGPGTARDGKPKFDLTQFDENYFSRLRERV
                     KLAQARGIYVSIMLFEGWGLQFSPNAYESHPFHPDNNINGIKGDLDGDGKAVEIHELK
                     DPRILEIQKAYVRHVIDTVNEFDNVLYEISNENHPPSTDWQYEMIRFIKAYESEKGRQ
                     HPVGMTFQYRGGSNKTLFDSPADWISPNPEGGYRDDPPAADGSKVIINDTDHLWGIGG
                     NRTWVWKSFMRGMNPIFMDPYDGRVLSRGSNLAWTEDVRKAMGDVRKWSQRVDLTSMT
                     PRPDLASTKYCLANPGKEYLVFFPEKTDGTEIQLVPGRYELRWFDTRARQEQPNGTLA
                     TSKESMFVKVPADGEWLLHVKAVQ"
     gene            6473..7858
                     /locus_tag="PLD05_06035"
     CDS             6473..7858
                     /locus_tag="PLD05_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77031.1"
                     /translation="MSRWSGSTALLGLVGIGLSLLHGSTGWASEVNRPNFLVIIADDM
                     ACEDCGAYGHPHIKTPNIDQLAREGMLFERAFLTCSSCSPSRTSLLTGRYPHATGAAE
                     LHQPVPPDQLLVTTPLREAGYFTAAAGKWHLGPHVKSQFDVVQEGGRDGAYEYWLPVL
                     KERPKDKPFFIWLATTDPHRPYGPNRIPEPHTPQDVIVPPYLPDVPEVREDLALYYDE
                     IGRLDTWVGRIREELIAQGVWDQTMVVFLSDNGRPFPRCKTTVLDSGVRTPLIVRWPG
                     RIPAGSVSSRLVSAVDLAPTILELAGITPGPSFQGRSFAKLFDDPTAKIRDYVYAEHN
                     WHDYQAYERSIRNERFLLIKNWLPQLPRTPPADAVRSPTYDAMKRLRAQGKLTPPQME
                     PFVVPRPEVELYDCENDPHNLTNLADRPEYSTIKSELLRALETWREQTGDSVPEPLTP
                     DKFDRETGKRL"
     gene            8526..8792
                     /locus_tag="PLD05_06040"
     CDS             8526..8792
                     /locus_tag="PLD05_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77032.1"
                     /translation="MNFFTWIREGVRQAVLLGVADAVDSLGTSPDGGDYRQRIGEYLR
                     ASPDAGQWKRLAISSPAASGGRKKLGRGLEDIQAVVNPPNDARK"
     gene            complement(8928..10169)
                     /gene="fabF"
                     /locus_tag="PLD05_06045"
     CDS             complement(8928..10169)
                     /gene="fabF"
                     /locus_tag="PLD05_06045"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOP77033.1"
                     /translation="MKRRVVITGMGVVTPLSCQVEDLWERLCRGQSGIRLIEGFDTSI
                     FRVKFGGEIRNWNPSGYIDAKEAKRLDRFAQYAIVAATDAIRQAGLDFNREDPYRCAA
                     IIGSGVGGLGEIEMQHRRLLEKGPERVSAFTIPKMMLNAASGCVSIQFGIKGPSYGVV
                     TACASASSAVVEAAKGIQFGEIDVAITGGTEAAVTPLGIAGFTAMSALSERNDAPEKA
                     SRPFDRDRDGFVLSEGAGIVVLEELEHAKRRGATILGELLGYGCSSDAGHITQPDENG
                     TGAARAMADALRNAGLNPEDIGYINAHGTSTPLGDRAETVAIKNVFGDYAKKVSVSST
                     KSQLGHLLGASGGVELIISLLAVRKGVIPPTINYETPDPECDLDYTPNVPRECRMKAA
                     MSNSFGFGGHNVSLIVGEFRD"
     gene            complement(10166..10438)
                     /gene="acpP"
                     /locus_tag="PLD05_06050"
     CDS             complement(10166..10438)
                     /gene="acpP"
                     /locus_tag="PLD05_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOP77034.1"
                     /translation="MASIEERVINIVAESLATEKDKITRDSHFVNDLGADSLDLAELI
                     MALEDEFDISIPEEVTSEIQTVGQAIDHIKAAVEGEGSTATSDEHA"
     gene            complement(10710..11480)
                     /gene="fabG"
                     /locus_tag="PLD05_06055"
     CDS             complement(10710..11480)
                     /gene="fabG"
                     /locus_tag="PLD05_06055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOP77035.1"
                     /translation="MEKDSPRIQVNLHGKVACVTGAGRGIGRAIALTLGRAGAAVACV
                     DVNGQAAESVAEEIRALGKAQAVGFACDVTNSEQVTQTVEAIVEKLGGLHILVNNAGI
                     TRDGLLLRMKDDQWDSVINVNLRGTFLFTRSAARYLIRAGGARIINIASISGLMGNPG
                     QANYSASKAGVIGFTRTVAKELASRQVTVNAVAPGFIATEMAQALGEQVLEEIKKRTP
                     LGRLGDPQDVADVVLFLASEAASFITGTVIRVDGGLAE"
     gene            complement(11609..12517)
                     /gene="fabD"
                     /locus_tag="PLD05_06060"
     CDS             complement(11609..12517)
                     /gene="fabD"
                     /locus_tag="PLD05_06060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOP77036.1"
                     /translation="MGRIAFLFPGQGAQTVGMGRRLYESLPAARALYERASKIVGYDL
                     ARVCFEGPAEDLDSTVISQPAIFVTSLAALESLRTEAPDVVLSCEATAGLSLGEYTAL
                     VFAGVLEFEDGVWLVQKRGEAMQDASDATPGGMVSILGMERSQVESLCEQARGEETLQ
                     IANLLCPGNIVISGSLRACERAAELATRMGAMKAVPLAVAGAFHTPIMRPADERLAAA
                     LEKVRLRKPRIPVISNVDARPHDDPEEIRQLLIRQVIEPVRWEDSMRYLIGEGFDSFY
                     EVGPGRVLRGLLRRIERSVTCQNIEV"
     gene            complement(12529..12753)
                     /gene="rpmF"
                     /locus_tag="PLD05_06065"
     CDS             complement(12529..12753)
                     /gene="rpmF"
                     /locus_tag="PLD05_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOP77037.1"
                     /translation="MAVPKRRQSNARTGARRAHDYLVVPPPSRCPNCNESVPTHVMCP
                     KCGYYMGRIVTDFEDIQRRQNQRQAKGKNL"
     assembly_gap    12959..13058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13067..13139
                     /locus_tag="PLD05_06070"
     tRNA            13067..13139
                     /locus_tag="PLD05_06070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13100..13102,aa:Arg,seq:cct)
     assembly_gap    14481..14580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14581..17004
                     /gene="cas10"
                     /locus_tag="PLD05_06075"
     CDS             <14581..17004
                     /gene="cas10"
                     /locus_tag="PLD05_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOP77038.1"
                     /translation="RLRIGPKDKRLTIIHPLSGQSSELEVGTLEEEKVAAAIKELLDG
                     IDSPKQRFLALWRLLPERLARIGSWYRLLPADLRNPDHTIWHYLDAAAGLKMAISEGD
                     EGALLSFALGPVQSFIEAARSVRDLWAGSMLLSWLTFQGMLPILEAYGPTAILFPSLR
                     EIPLVDWWLIEQGLNKVDVPRSEWIRLPCLPNRFLALVPCESGGEQAEDLAERCQLAV
                     RDAWRGLAKSVREEIERSLAKLGEDFSGWVARWDTQIEGFFSVTTAVLPLRQCNEAMI
                     ARLFGASNFDEAFPKLAAVRRLAEAIPPGDRPGYDQTSAGRWQAQVEMSARLMEALRA
                     VRHIPPSTRDTPVPGKCSLMGTFEQMGPAKLEESRRFWETMAQCVHIGLDRIRKRERL
                     CGVAIVKRFAPHFLAQKLKIDERLLRTPDTATVAATRWLKEACDRGYELDPEAIRERY
                     DVWSGQWLFWTRKDQGQDEDEDAVPDDVWEEIQKARKDKSLGPPPTYYAILMMDGDWM
                     GRWLSGEMAPPIENVLHPETLSYFRQLNDPGAEAALRAPRPLGLVDHAVMSQALTNFA
                     LFVVPKIVKEHHGTLIYAGGDDVLAMLPASTAVACARTLRLAFSGDPRVNGGARPGYY
                     RLGNRELLMMGPRATSSCGIAVIHYKEDLRFALQQARRAEKAAKRAGRNLLQIAICRR
                     SGEHTSVLCPWEFVPTVERWVQAFLRGASDGWTYRLRAELKTLAAVSREAVRAELSRL
                     VERSEQSTKDLLSESENISAGQMLVRQFDDYLSQLLAPPRSLDEEQAMEGFVHLCQAA
                     SFLARGRTE"
     gene            17004..18209
                     /gene="cmr3"
                     /locus_tag="PLD05_06080"
     CDS             17004..18209
                     /gene="cmr3"
                     /locus_tag="PLD05_06080"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr3"
                     /protein_id="HOP77039.1"
                     /translation="MNQSILSAKTRLVALRLDPLDVLFFRDGRPFHEASRGESGLPRP
                     QTLAGALRTHLLRRAGCDGDGFRKLGEYLRAGQSLEQAITSVCGAGWIARVEVRGPWL
                     CEEVNGTLEILVSIPANVQEIEGGMETSSVSESTTAEKNVRSGVRGNSDRRWVRFDPL
                     EPELDLPGWKPPVAGMRPLWPRLHARSQRRSGYLRPAGLAKFLAGEDPPPETVVSPED
                     LYGFDQRTGTAIQPDQLTIEEHLFYAVSLLALRPGVAFYAEVNLPSDAPSEILRGETL
                     LDFGGEGHKVLAREVAPFMWPDPPDASNGRVLLLLTTPVVFSKQWCPRLPAGTRLVAA
                     AVTGYEAFSGWDLARGGPKPTRFAVPAGSVYFLETSDQRVLISSLAESDEDRQLGFGC
                     VVQGVWNYV"
     gene            18202..19170
                     /gene="cmr4"
                     /locus_tag="PLD05_06085"
     CDS             18202..19170
                     /gene="cmr4"
                     /locus_tag="PLD05_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOP77040.1"
                     /translation="MSNSLNGRSAATLFLHAQTGLHPGTGTALGVVDLPVQRERHTQW
                     PTIAASALKGILRDVCREKAKADFPNGETLSPRRWANERHQKLVAAFGPGIPEESSEH
                     AGAVALTDARILAFPVRSLRGVFAWVTCPAAVQRLNRDLALTGLQPLDDIPAVLDNEI
                     ACTSSSPLLVSRNPDKVVLEEFDFNRIQATCDQLAEWMAHHAVADAGTQSRLRWHLAV
                     LSDNDFSYFVRHATEIVARIALDYERKTVKEGALFYEEFLPAETIFYTLILASESRKP
                     DVKLSAAEILEFVRRCVEAAGVIQIGGDETTGKGLCTVRWLQNEEA"
     gene            19174..19584
                     /gene="cmr5"
                     /locus_tag="PLD05_06090"
     CDS             19174..19584
                     /gene="cmr5"
                     /locus_tag="PLD05_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOP77041.1"
                     /translation="MSHGTRDRLTIDQQRARHAWRAVESLPVDSEEADDYAREAKKLP
                     VRIITSGLGQALAFVNAKKAKKQGLAKLEEHLTDWAKQRLEVAFPDNKTLLDVIVHND
                     SDFLRRATEEMLAYLVWLNRFTEAKGLPKKGEQD"
     gene            19584..22010
                     /gene="cmr6"
                     /locus_tag="PLD05_06095"
     CDS             19584..22010
                     /gene="cmr6"
                     /locus_tag="PLD05_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HOP77042.1"
                     /translation="MAPTGIPLPESVVKLIQSVPLNERHPGLQLDKYIEAPASQEQQK
                     ESLKSVLEAATDEHLLQEVTRRREAILQQLGAVRFTAKTAGPMTLHLARASALENAGI
                     CLHSLYGFVYFPGSGLKGMARAYAETVWLPLQSDQQKAWRQIEDVFGWASNPTRRQQI
                     SDPNHPAQVRRVNDSDPKSSEISASAGNIVFHDAWPVRWPKLQLDIVNNHHTEYYRNE
                     QAAPGDWEDPEMVSFLAIGPGEEFNFALSKRRSDVADKLLYLAALWLAGALTYEGLGA
                     KTAAGYGAFIVSSPPVPAPKPPAREVFEATLELVTPAFLAGPNQNADNCDLRPATLRG
                     LLRWWWRTMHAGYVDLPTLHRLEAAVWGDTNCGGPVRITVQREDPSSPSPVPVPGKSI
                     RKDKNNRDVLRPDQEFIRQQKLALPPDQKTTHGFLYLSYGMDEMPAGKPEERRRRFIM
                     PPGAKWRVRLMARDGFYRPVGASASAKPVRIPAAVILEQAKAALWLLCHYGGVGSKGR
                     NGFGSLVLLDDGDTQNKRGELERAAAEFRHICGVNEQIEPHCQIPALRTALPEVLVST
                     PWKNHWFVLDQLGYSLQSFAQKYKRKWIKEALGLPRRIGISDDDGSQSKHYQRELDQK
                     TKQPVVWLGQKHPYRGNRKPKDMRHAAPVHFHIARSADGTYQIRVIAFPCAVLPDWKT
                     SNDVLQKLLAHLREDLQKRCDENSRDSTLVSPAIVSRALPRIPEKRPAGTPVQVKILA
                     ERPKGGYEVQEEGKPQGTLTVGTPPTERPEVGDIVSVEVHNDDPRRPQYRWPQNRPPM
                     QSDKYPSSRR"
     gene            complement(22058..23020)
                     /locus_tag="PLD05_06100"
     CDS             complement(22058..23020)
                     /locus_tag="PLD05_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77043.1"
                     /translation="MGVLVLTRLWTMELIGEGRLRPWLGPAIRGALAWAVKEAVCRWP
                     PLERDDRYKTCRGCQYLGDCAYGSTFEAEGTLSEGAPSGMADGQRAITIRPPFPVPEY
                     GRPGDRMTLCATFLGERAAAAADVINRVLLESDRGFELGRDRVGFRLSAYLTEKDAPG
                     ALQCIEPEHLPASPEDCRGYVPAVRLDLITPLFLKEEPVEGGKARAVLKPTLGQLVRG
                     CLRTVGRAFAAFGKGLDGRVDFAGLKKCAEAVPTQAAFWQPFRQRHHSTRQRQRYDLV
                     GVTGGAVFGRVPLCLLPWLVWGGRLGIGEHRVTGAGCWEVTLLA"
     gene            complement(23038..23382)
                     /locus_tag="PLD05_06105"
     CDS             complement(23038..23382)
                     /locus_tag="PLD05_06105"
                     /inference="COORDINATES: protein motif:HMM:NF020530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1874 domain-containing protein"
                     /protein_id="HOP77044.1"
                     /translation="MAECHRVKGKVYVLNAPVLSDYGDWRFEGPISVAEAVELLSEGF
                     ISAVGHVGAAEFLSKRLGMPVSVQRFRAEMQPGDRALVLRIKCRLDEGAILTAEEMEK
                     LPVELGILTRLA"
     gene            complement(23606..24955)
                     /locus_tag="PLD05_06110"
     CDS             complement(23606..24955)
                     /locus_tag="PLD05_06110"
                     /inference="COORDINATES: protein motif:HMM:NF021189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HOP77045.1"
                     /translation="MTPKTILICTVGTSLVQPNLERLKSQFEGGSLAPQHRQLVEAYI
                     SGDTRGVAQALRRLPPTDRLCGAEINSIAGMIERGYVDPKCGLYFLHSETERGRQISQ
                     ILKEYYRAVDHQPVELIEVADLQDDEPKRFRSKGLRNLVREIATVVRRHSPLACAINA
                     TGGYKAQIAIAVVLGQALGVTVYYKHELFSEVIAFPPLPVALDFQLWMKASGLLSELA
                     SDTHELLPASRYQDEWDERYESLVERVKIDDTEYLELSPAGQIFYETFKERFHSVKDL
                     VVPPLAPTKRPPRLERAGWPGQHPEIEAFMRRVTEEVPFVVQCETFYFNPDLSERTRF
                     LVSAKGIVGVYSDGRYCVKFLVETTAQTDGQRAAAVAALNEWLKDPNYFRSREEIKAT
                     EAIKERDEALAAWERTQAQLRELQQQNEELRRERAKLQETIERLSAELAQARGQHQG"
BASE COUNT         5481 a         7440 c         7187 g         5556 t
ORIGIN      
        1 cggaaatcgc caggaaaacg tgccgtgttg ttgcacccga cggtaggttt ttccaagtat
       61 ctcctccatt gtgcgggagc agtggttgcg ggtaatcgtc tcgtggaggc gacggcgcac
      121 ccctcgaggg ggttatgtcg ggagggtcgc cgtcctcggc gaccgctgca tctcatctgg
      181 actggccagg tgaaactcaa tcatggggag attttccgta gcgagcggtt ctgagccgtt
      241 ttgtcgccgc aaagaattca catatcgcag gagattggtc actgagtgcg cacgaccgag
      301 tccgcccgac agacaattcc acgatggccc cctcgctcag gatagcggcg atgagctgct
      361 tcgacttcga gatcatcgga acctagagtt ccgaaaaggg ttatatcttc aagagagaaa
      421 acgtcaccaa attatgttcg gggcttctgt tcggggttcc ccggttggat cgggcgcctg
      481 ctgatcgtga gttgggggcc aaaactgcgg tcacgaaaat ggacgtttag ggagggttcc
      541 tcaaatgacc atgtcagtgc gaatctatct tctcgtgaca atcctattga ttatcggtcg
      601 gtcgccgaac atcgtggcct tggagcgtcc tgccgagcct gtgtacgagg tcgtgagtcc
      661 tttgggggag cgggttgttg aggcgggggc catggcccct aggttggaaa cgctcaacgg
      721 caagacgatc tgcatggtat ggaatcatgc gttcaaagcg gatgtgactc tcccggctat
      781 tgccgaggaa ctgaaaaaac ggtttcccga cataaaaatt gtcccctatt attccatgcc
      841 tctagctccc ttgccggaac ttcctggatc acccacgaaa gagtccgaga atctgcgagc
      901 ggctttgaag gccatgggcg ctgatgccgt gattgtggga aatggcggct gcggcatttg
      961 cacgccccgc gcgtgctatg ccacggtggt ggccgagcag ctcggcatcc ccgccgttgt
     1021 ggtgacaaat cctggcttcg atgaacaagc ccggaccatg gcccaatact cgggcattcc
     1081 gtcgctccag gtcgccgtat atcccgatcc ttttgatctg gaaacggagg cgcaactggt
     1141 cgagaaatcg aagacgatcc tcgtgcctca gattattgat gctttgacaa aatcgatcgc
     1201 ggcggcaaaa gattctgtta aaatcgatag ccggaagatc gtatttactg ggacactcga
     1261 tgagattaat cgctatttca cggccctcgg ctggagtgat ggattatcca tttcgccgcc
     1321 gaccgtggat cgaatagagg aatttctcaa atacacggat tatggccctc acgaagagat
     1381 cgcggtccta ccgccagcaa atctcaaggc gacaccgtgg acaatcgccg ccaatggtgt
     1441 catggccggt tgccgtccgg agtacatgcc gattctaatt gccgcggtgc aggcgatggg
     1501 tgatcctgct ttccggtaca gaagtcatgg ggctagcacc cattcgatca caagtttctt
     1561 ttgggtcaac gggccccttg cgagacaact cggtattgat catggtcagg gactcattgc
     1621 gcatcccgtc aacgctgcca ttggacgtgc catgagcctc attgagagga atatcgcagg
     1681 gctaagaatt aaggaaaccc agatgggtag cttcggcaag atgatgtcgt gggtcctggc
     1741 ggaagacgaa caggctatga tccagtgggg gtgggaaccg taccatgttg agaagggatt
     1801 tgatagaaat gctaatacca tcactgcggg caccagcacg gtttggggcc gaaatcttgt
     1861 gcccgcgaca tccgagcccg aaataattac ccaactcatc gcctatgacg ctgtcaacac
     1921 ggaggccttt gccagcggct ttacgggtca aagtcggcgc tatgtgctaa ttggacccgg
     1981 cgttgccgaa atccttgccg gtggaggtta cacaaagggt accttgaggc gagatatcat
     2041 gagaatgggt cgaaagatca cttacgaggc ggtcttttcc caggtctatg gctcgtttgg
     2101 ccgagtgcaa cgttccttcg aggaggaact tcgacgggcg ttgcgctaca gcgaaaaagg
     2161 gaaactgccg ccgtggtacc ctcgattccc cggttgggaa gagattgaaa ccgtgccggc
     2221 cgtgggcatg attgaattca tcgtctgcgg agatcctatc aggaataagg ttcaaatcct
     2281 cgccggtggc ccgggcatcg cgagcaaaga aatcaaattg cccgccaact gggatgaact
     2341 catgcagaaa gcaggatacc gtccgctgcg agagttctat cttcagtagc gatggtttac
     2401 ggaaaagccg ttggcggcca gtttccggta gtgcgttgga tctgtcagcc tgtgtctgag
     2461 ttcgcgagct aaatacctcc acctgggcag ttcagtgccc taactggcga gcttttaccc
     2521 atcgcccgaa aactctcggt ttgccgggaa aaatcgccgt gttgttccac ccagcgttgc
     2581 cttttttcaa ggattgcctg tatcgggagg gtcgccgtcc tcggcgaccg ctggatctat
     2641 tgcatctatc ctcggcgacc gcgcgaggat cagcgtctgc gatgacccaa tcaccggacg
     2701 gcgtggacgc cgtccctccc ggggctacac tatcatcgac gcctgtcaag tggcgtacgg
     2761 gggccgttag gaatggcgtc ccgctccttc gattcggcga aacggtcagg taccgtttct
     2821 cgtaattgtc cgccgtagtc accttcgcgc tctgtcgcgt ccgccgtgtg gtctcgcttg
     2881 ttagtcctgt ccgttgccaa tctgggaggg acgcggtccc cggcgtcctt tggtactagt
     2941 ttggcgtgcg tcatggtcgt tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnntagggca ggcacggggg cctgccccta cggtttcaat cggtgcgtgt aggggcaacc
     3121 ccccgtggtt gcccctttat gcgcagcgcc cagggttcgc gtttatgcgc tcattcgttg
     3181 tttgaatttg acggggaccc gggcgctggt tacgttctgg ggcctgcccc tacggtttcg
     3241 atggaatgcg cgtacgggca accccctgtg gttgctcacc gtggtcactt tcgcctggtg
     3301 gcacgccccg cccccacgag aatctgttga gacaggcaat aaccgtgagg cagatggacc
     3361 agactgccgc cgtgttttat taggggcagc acatggggcg ttatggttgg gcagtgcttc
     3421 cctcttctca acgggcgaca atcttagcca aagggttgct tttgcttgtg gctgtccaca
     3481 taatagggca cacctgatga gttccacatc gttttccctc aactcaccta tgtcgggagg
     3541 caaatcatga gtcgttcact gaagcgatgt gtcggggtgt tgtttttgtt ttctcttggg
     3601 tgggtaggtc acaacctact ggccgaacag gcgggcatct ctccttggtc gaagaacccc
     3661 tattattggc agtacaatgg caagcccgtc atgctcattg ggggaagcaa agatgacaac
     3721 ctcttccaga ttcccgatct gaaagagcac ctcgacgaga tggtagccgt tggggccaac
     3781 tacattcgga atacgatgag cgatcggcca gacaagggct ttgaggtcta tcctttcaaa
     3841 aagctagaga atggcaaata cgacctgaac cagtggaacg acgaatactg gcagcgattt
     3901 gagaatcttc ttaagtggac gaaggagcgc aacatcttcg tacaaatcga ggtttgggat
     3961 cgattcgatt actcgcggga caattgggag ccccatcctt ataaccccaa aaacaatatc
     4021 aactacacct acgaggaatc gggatttgcg gaacattatc ccgaccaccc gggcagcaac
     4081 aaacagccct tctttttcac cacgccctat caacgtaaca acaaggttgt gttcccttat
     4141 caagagcggt tcgtcgataa gatgctctcc tattccctgc aatacgatca cgtgctttac
     4201 tgcatcgaca atgagacgtc gggcgaagaa gaatgggcaa aatactgggc ggagcatatc
     4261 cgacgaaagg ccgcacagat cggtaagaag gtctgcgtga ccgaaatgtg ggacgactgg
     4321 gatcttaaag cgccgaggca tcggcgaacg ctggaccatc ccgaacttta tgcatttgcc
     4381 gacatctctc agaacaatca tcaaaagggg caaacgcact gggataattt ccagtgggtt
     4441 cgggattatg tgaaaaatca accgcggcct ctcaacagcg tcaaaatcta cggggccgat
     4501 acaggtcgtt acgggaacga tcaagatggc gtcgagcggt tctggcgact ccttctcggg
     4561 ggcgccgcgg cggtccgttt ccaccggcca gactctggat tgggcctgaa cgacaaagcg
     4621 aaggcttcga ttcaggccgc gcgaaaggtg gaatcccttg tcccctggtg ggaacttcgg
     4681 ccggccatgg aactgctcgg caatcgtgaa gccaacgagg cctatgcggc ggccaaaccg
     4741 ggcgaggcgt gtgtggtctt cttctgcaat gggggcgagg tgaccgtgaa cctctcccag
     4801 cgggcggcgg gagaagcgaa actccgttgg ataaacgtct ccgatggtca gtgggggcca
     4861 gaaggaacaa cgaaggccgg cggcctcgta acgctcaaga cgccccaggc cggaccgtgg
     4921 gttgccgtta ttctccggaa ctccccagca gtggcggccc ggcagagtgg gtcaagcgat
     4981 gtgaaacgct ctgtcccggc aacgctgatt tcctgggcag aaaaagcccc ccaaaaacaa
     5041 accgctcacg gtgttttgcg cgtttgcccg gcgaatccgc gttacttcac cgacgactcg
     5101 ggaaaggcga ttctcatggt aggttcacac acatggaaca atttggttga catgggaccg
     5161 ggtgatccgc cgccggcgtt cgattttccg gcctacctgg actttctcga aaagtacaat
     5221 cacaacttca ttcgcatgtg gacgtgggaa ctgctcacct gggacacggc aggcaaccgt
     5281 gaaccaaagg cccaggtgca cgtcgccttt ccgcatccgt ggaaacgaac cggtcccggc
     5341 actgctcgtg atggcaagcc aaagtttgac ctgacacagt tcgatgaaaa ctatttttcg
     5401 cggcttcggg aacgtgttaa gttggcccag gcaaggggaa tctacgtctc aatcatgctt
     5461 tttgagggtt ggggccttca attctcacca aacgcctacg aatcgcatcc ttttcatcct
     5521 gacaacaaca tcaacggcat caagggcgat ctcgatggcg atggcaaagc cgtggaaatc
     5581 cacgaattga aagatccccg aatcctcgag attcaaaagg cctatgttcg tcatgtcatc
     5641 gatactgtca acgagtttga taacgttctg tacgaaattt ccaatgagaa tcatccccct
     5701 tcaaccgact ggcaatatga gatgatccga ttcatcaagg cctatgagag cgagaaaggc
     5761 cggcaacacc cggtggggat gacgtttcaa taccgcggtg gcagtaataa aacgctattc
     5821 gacagtccgg cagactggat ttcgccgaat ccagaagggg ggtatcgaga tgacccgccg
     5881 gctgccgacg gttccaaggt cattattaac gacacggatc acctttgggg gattggtgga
     5941 aatcgcacat gggtttggaa atccttcatg cgcggtatga atcccatctt tatggatccc
     6001 tacgatgggc gagttctttc gcggggatca aacttggcct ggacggaaga tgtgcgaaag
     6061 gccatgggtg acgtgcggaa atggtcgcaa cgtgttgacc tgacgagcat gacgccacgg
     6121 ccggacctcg cgtcgaccaa atactgtcta gcgaatcctg gcaaagagta cctggttttc
     6181 ttcccggaga agaccgacgg tacggagatc cagttggtgc ccggtcgtta cgagcttcgc
     6241 tggtttgaca cccgtgctcg gcaagagcaa cctaacggaa ctcttgccac gtcgaaggaa
     6301 tccatgtttg tcaaagtccc ggccgatgga gaatggctcc tccacgtgaa ggcggtgcag
     6361 tgaacaagcg ttgtgtccca atcaggcggc tcacgatgac gccgtccgtt tggtgagcaa
     6421 gagattttgg tcattaggcc tattagggga ggattttcac atgagggtgt ttatgtcgcg
     6481 ttggagcggc tccacggcgc tattggggct tgtgggaatc gggttatctt tgctacatgg
     6541 gagcacggga tgggcaagcg aggtaaatcg ccccaatttt ctggtcatca tcgccgacga
     6601 catggcgtgc gaggactgtg gggcctatgg ccatccccac atcaaaaccc ccaatattga
     6661 ccaactggca cgtgaaggga tgctttttga gcgggccttt ctgacgtgca gttcatgcag
     6721 tcccagccgg accagtctcc ttacaggtcg ctatccccat gccacagggg cggcggaact
     6781 ccatcaaccg gtaccgccgg accagcttct cgtcaccact ccactccgtg aggcgggcta
     6841 tttcacggcg gcagccggaa agtggcatct cggtccccat gtaaagtcac agtttgatgt
     6901 tgtccaggaa ggcggacgcg acggggcata cgagtactgg ctgcccgttc ttaaagaacg
     6961 ccccaaagat aagcctttct tcatctggtt ggccaccact gatccccatc ggccatacgg
     7021 ccccaatcgt attcccgagc cacacactcc gcaggatgtg atcgttccgc catacttgcc
     7081 ggatgttcca gaagtgcggg aagacctggc cctttactac gacgaaattg gtcgattgga
     7141 tacgtgggtc ggtcggattc gggaagagct gatcgctcag ggggtttggg atcaaacgat
     7201 ggttgtcttt ctttccgata atggcaggcc tttcccacgt tgcaaaacga cggtccttga
     7261 tagcggtgta cgaactccct tgatcgtccg ctggccggga aggattcctg ccggtagtgt
     7321 gtcatcgcgt ttagttagcg cggtggactt ggcaccaacg atcctggagt tggccggtat
     7381 tacgcctggt ccttcatttc agggacggag ctttgccaag ctgtttgacg atcccaccgc
     7441 aaaaattcgg gactacgttt acgccgagca taactggcac gattatcagg cgtacgaacg
     7501 gagtatccgc aacgagcgat tcctcctcat caagaactgg ctaccgcaac ttccacggac
     7561 accacccgcc gatgccgtac gaagtcccac ctatgatgcc atgaaacggc tgcgagctca
     7621 ggggaaactt acaccgccgc aaatggagcc cttcgtggta ccgcggccgg aggtggagct
     7681 gtatgattgt gaaaatgatc cgcacaatct gaccaacctc gcggaccgac cggaatactc
     7741 gaccataaag tccgaactcc tgagggcctt ggaaacctgg cgagaacaga cgggtgactc
     7801 tgttcccgaa ccattgacgc ccgacaagtt tgatcgtgag acgggaaaac ggttgtaaat
     7861 ctgggataaa agggccgtca ttaatgccta tcccataatc tcccgtcgcc cgttcggcgg
     7921 gagacgggtc gggggtgagg gtggcttgaa tcactggcgg ttttctcaat cgattccgac
     7981 gatcgcttgc cctcacccca accctctccc ggagggagag ggagactttt tgggatggac
     8041 tctaaatcca cgggataaca gataagccag gcaacgcaga taatcgaggc atttcgaccg
     8101 ggtagtgcgc actggcggtt ggtgatagcc gtttcgcgcc cgaacaaaaa acagacatga
     8161 cggcgtccct tcctccggat tgagcagtca gtcctcatct caggatgggg ggaccaattg
     8221 tacgttctcc aaccgtgata cgggcaaaac ttatggccga tccaccgtgt gggagatcac
     8281 gcgccccggt ttggcctttt tgcccagtgg agggccacac tttgtcgtaa ccgtaccaaa
     8341 ctgtgcgtct tccaatttcc cgcgggggaa aacctacgga ttacttcttt tttccgcagt
     8401 tacccactcg gccacctgaa ggaaatcgct ctggttcggc gcgacggatt ttgctaatct
     8461 ctcatctcct tgggtccgcc gtacaggagc agattccttt agaaagaaaa ttgcaggagg
     8521 aacacatgaa cttcttcaca tggattcgcg aaggtgtccg tcaagccgta ctattgggtg
     8581 ttgctgacgc cgtcgatagt ctggggacgt ctccggatgg aggtgactat cggcagcgga
     8641 tcggcgaata tctgcgggca agcccagatg ccggacaatg gaaacgatta gccatttcat
     8701 cgcccgctgc cagcggcggc cgaaagaagt tgggacgggg actcgaagat attcaggccg
     8761 ttgtgaatcc tcccaatgat gcccgcaagt aactcccacg gattgccttt cgcaattcgt
     8821 tagtatcgca gcggcgacca ggggcgaaaa atgaacaaac ccggctcgga cgtatcttcc
     8881 ggaccgggtt tattttttga cgtcttcgct tacgtcggcg aaagagccta atcccggaac
     8941 tcgccgacga tgagggaaac gttgtgtccc ccaaaaccga agctattgga catggcagcc
     9001 ttcatgcggc actctcgcgg cacattcggc gtatagtcga ggtcacactc tggatcgggc
     9061 gtctcgtaat tgatagtcgg cggaatcaca cccttcctga cggcaaggag agagatgatc
     9121 aactctacgc cgccactcgc ccccaacaga tgtccgagct gactcttggt gctggacacg
     9181 ctcaccttct tggcgtaatc gccgaacacg ttcttgattg cgacggtctc agcccgatcg
     9241 ccaagtgggg tactggtccc atgagcattg atatagccga tatcctcagg gttcaagccg
     9301 gcgttacgca gggcatccgc catagcgcgg gcagctcccg tcccgttttc atccggttgg
     9361 gtgatgtggc cggcgtccga actgcacccg tagccaagca actcaccaag gatcgtcgcc
     9421 ccgcgccgct tggcgtgctc cagttcctcc aagaccacaa tcccggcacc ttcgctgaga
     9481 acgaatccgt cgcggtcgcg atcgaacggg cgactggcct tttccggggc gtcattgcgc
     9541 tcggaaaggg cgctcattgc agtaaaaccg gcgatgccca atggggtaac agcagcttcc
     9601 gtaccgcctg taatggccac gtcaatctcg ccaaattgga ttcccttggc cgcttccaca
     9661 actgcgctcg aagcactggc gcacgccgtg acgaccccgt aacttggtcc tttgatccca
     9721 aattggatcg agacacagcc actcgcggca ttcaacatca ttttgggaat cgtgaatgcc
     9781 gatacccgct ccgggccttt ctcgagaaga cggcgatgct gcatttcgat ctcgcccagg
     9841 ccgccgacgc cagagccgat gatggccgcg cagcgatacg gatcctcgcg gttgaaatcc
     9901 aacccggcct gccggattgc gtccgttgcc gccacgatcg cgtattgggc aaaccggtcg
     9961 agccgtttcg cctctttggc atcgatataa ccgctgggat tccaattccg gatttcccct
    10021 ccaaatttca cgcgaaagat ggaggtgtca aatccttcaa tcagacggat cccgctttgc
    10081 ccccgacaga gtcgctccca gaggtcttcc acctggcaac tcagcggtgt gacgacaccc
    10141 attccggtaa tgacgacacg tcgcttcatg cgtgttcatc cgaggtagct gtgctgcctt
    10201 caccttccac tgcggccttg atgtggtcaa tggcctgtcc gacagtttga atttcggacg
    10261 tcacctcttc cggaatggaa atgtcgaact cgtcctcaag cgccatgatc aactcggcca
    10321 aatcgagcga gtcggcaccc aagtcgttca caaagtgaga atcgcgggtg attttatcct
    10381 tttccgttgc caaactctct gcaacgatgt tgatgacacg ttcttcaatc gatgccacga
    10441 cagtcatcct ccttttcttc tcaattgcca accgccgatg gggacaagca cgcccaaacg
    10501 gccaatactc cactgactca cttccggatt gaagcgattc ggaaacgacg tctcccccaa
    10561 aacaaaatac gccccttttt gcctcaaccg caattaaatt acgttacagg gttttctctt
    10621 aagtgtccat accgagaagc ccctcgtgaa gcggcaccac atcctttccc cactggccgc
    10681 caacccacgc ggcgctggcc tgttccctat tactcggcca gaccgccatc aactcggatg
    10741 accgtccctg tgataaaaga agcggcctca cttgccagga ataaaacgac atcggccaca
    10801 tcttgcggat cacccagccg ccccaaaggt gtgcgtttct tgatttcttc cagcacctgc
    10861 tctcccaaag cctgggccat ttctgtggca ataaaacctg gcgccacggc attcaccgtg
    10921 acctgtcgcg aggccagctc cttggctacc gttcgggtaa acccgatgac tcccgccttg
    10981 gatgccgaat agtttgcttg gcccggatta cccatcaacc ccgaaatact ggcaatgttg
    11041 ataatccggg ctccaccggc acggatcaaa taccgggctg cactcctagt aaacaggaac
    11101 gttcctcgta agttcacatt gatgacactg tcccattgat catccttcat ccgcaacaag
    11161 aggccgtccc gcgtgatccc ggcgttattc actaggatat gtaagccacc cagtttctca
    11221 acaatggcct cgacggtttg ggtaacttgt tcactgtttg taacgtcgca tgcgaaccca
    11281 actgcttggg cctttcccag cgcgcggatt tcttcggcca ccgattccgc cgcttggccg
    11341 ttcacatcaa cacatgccac ggcggcccct gcacgtccca acgtcagggc aatcgcccgg
    11401 ccaatgcccc gtcccgcccc cgtaacgcag gccaccttcc catgtaaatt gacttggatc
    11461 cgcggagaat ctttttccat gacctattct cggcctcaca ctgatactcg cttctggtgt
    11521 ccccttgccg aaaaaatctt ggcttgcgct atccctattt ggacatttat gcctaactct
    11581 tgtggcaaca gccttggcca atactcgatc acacctcgat attctggcac gtgacactcc
    11641 gttcaatccg acggagcagc ccgcgcagca cccgccccgg ccctacttca taaaagctat
    11701 cgaatccctc gccgatcaaa taccgcatcg agtcttccca acgaaccggt tcgatgacct
    11761 gccgaatcag cagttggcgg atttcctccg gatcgtcgtg ggggcgggca tctacatttg
    11821 aaataacagg gattcggggt ttccgcaatc tgactttctc taacgccgcc gccagtcgct
    11881 cgtcagccgg ccgcatgatc ggcgtatgaa acgcccctgc cacggccagg gggactgcct
    11941 tcatagcccc cattcgcgtc gccaactcgg ctgcccgctc gcaagcccga agggatcccg
    12001 aaatgacaat attccccggg caaagcaaat tggcaatttg aagggtttcc tcgcctcggg
    12061 cctgctcaca aagggattcg acctgtgatc tctccatgcc aaggatgctc accatgccgc
    12121 ccggagtggc gtctgaggca tcctgcatgg cttctcccct cttctgaact agccataccc
    12181 cgtcttcgaa ctccaaaacc ccggcgaaca ccagggctgt atattcccca agactaaggc
    12241 cggccgtggc ctcgcaggaa aggacgacat caggcgcctc cgtccgcaac gactcgaggg
    12301 ccgccaggct cgtcacgaag atcgccggtt ggctgattac ggtggaatca aggtcttccg
    12361 ccggaccttc aaagcatacg cgagccaggt catacccgac gatcttgctc gcccgctcat
    12421 acaacgccct cgcagcgggg agggactcat aaagacggcg tcccatcccc accgtttgcg
    12481 ccccttgacc aggaaatagg aaagcgattc gtcccaaagt cgcctcacct ataagttttt
    12541 gcctttcgct tggcgctgat tttgccgccg ttggatgtct tcaaaatctg tcacaattcg
    12601 gcccatgtag tacccgcact tcgggcacat aacatgcgtc ggtacgctct cgttacaatt
    12661 gggacaccga ctaggcggcg gcaccacaag gtagtcgtga gctcggcgag cacccgtcct
    12721 cgcgttactc tgtcttcttt ttgggacagc cattgagatc cctcacgtga gccaagaaga
    12781 attttccgga taacgttggt cggtcagctt gacgatagcg gcgcgcaaac ccgctcttcc
    12841 gcccaaaccc ctagggcacg atatactgtt tgcgcccgcc aaatgcgtaa aagctttatc
    12901 ttaagaccct tgtccatatg cgtcaacgcc gggcgacagc cgaggaaact gccgcattnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca agttatgcgc ctgtagctca
    13081 ggggatagag cggcggtttc ctaaaccgta ggtcgcaggt tcgaatcctg ccaggcgtac
    13141 ctttctcgcg ccaacacgaa gccgttaagc gggggtaaag tctctgcagg ccacagctca
    13201 tcgtgccctt cccgcgggcc tagaataaga gtccgaattc tcctctcagg gccttgagtt
    13261 ttcggcaaac ccgaggcttg ctatgtcttc gccccggtag ggctactcgg ctggaaacga
    13321 gtgtttcctg aagtagcgaa aagggatgtc gagtcccaat tcgtcaagag gttttgctat
    13381 ggttgggtcc aaagtgcaac cattgatacg tttacgccct accctatttc gggaaactga
    13441 aactggcttc gcgtgtatgc aaaggccact cgtgcaattc gtagtagggt gcgtcgcgga
    13501 gttaccacaa agatttcttg agcccggtaa aaccactttc agtcctaaac gtatggtacg
    13561 tcacgctccg ataaccgcta catgagatcg tgacggactc gttcggcacg aaagaccaac
    13621 cttccagaat gccgatctct accgtcttgt gcggtccaat ggtgacgtca agcgtcgcct
    13681 tctggttgaa atccttattg gacatgacca attgcacggt aagggaccta cccgttaggt
    13741 tcgtgagtag gagcacacta gttgatgggt ctagcagcga ggggcgcatg gacacgtcga
    13801 tcacagttgt gatcgaaggt gtggtgctgt cgcaacccga gagcgtcgta acgagggcta
    13861 ttacaatggt gacagttgca aagtggacag gccgcataat atgcccccaa cgtttggcga
    13921 atgtaacggt tgacggggtt cctattcttc cacgccggct ggaatcccac ggcagccgac
    13981 cgacagtcgt gattttaaat gccggatttg gctcgtcaag cgctctgcaa aaatcgcgga
    14041 tttcttgtgt tcgccgggcg ccgattcgct ctaagctggc gttcgtttag aaagacgcgc
    14101 tctgtcgcct ccgcggtccg gtaccgtcgg ttggtcatat ctgctggcaa tctgggaagg
    14161 acgcggtccg ccgcgtccgc cgtgtttttg ggggggcgtt tgacccgatt cggctacgac
    14221 gccgcgtggc ggtccgtcgc cacgggggag atgcggtcac ttgcctctgc tgtgtcatac
    14281 cacgggtttg ttgtgttcgt cggcagcact agtaggtttc gcgcttaacg cactagggtt
    14341 gcgccgagcc gatatttacc cccttagtga gaaaagggcg gttatttcct taccctaggc
    14401 tgcggtggcg gctatctgga aacatcggag cggtttgggt agattatgtg ccatctgacc
    14461 acgattttgt gtcggtggat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 aggctgagga ttggcccgaa agataagcgg ttgacgatca ttcaccctct gagtggtcag
    14641 agttccgagt tagaagtcgg gacacttgag gaggagaaag ttgcggcggc gatcaaggag
    14701 ctcctcgacg ggattgattc gccgaagcaa cggtttctgg cgttgtggcg gctcctgcct
    14761 gagcggctcg cacgaattgg gtcatggtac cgcttgctgc ccgcagacct aaggaacccc
    14821 gatcacacga tttggcatta cctagatgca gcggcagggc taaagatggc gatatctgag
    14881 ggggatgagg gggctttgct ttcgttcgcc ttagggccgg ttcaatcctt tattgaggcc
    14941 gctcggtccg tgcgggacct ttgggcagga agcatgctgc tatcatggct cacctttcaa
    15001 gggatgttgc cgatcctgga ggcgtatgga ccgacggcaa tcctttttcc ttctctacgc
    15061 gaaattcctc tcgtggactg gtggctcatc gagcagggcc tgaataaggt ggatgtgcca
    15121 cgttcggaat ggattcgctt gccttgcttg ccgaatcgat tcttggcctt ggttccctgc
    15181 gaatcgggag gggaacaagc tgaagacttg gccgaacggt gccaattggc ggtgcgggac
    15241 gcatggcgag gtttggccaa atctgttcgc gaggaaatcg aaagatcgct ggcgaagtta
    15301 ggggaagact tttcgggctg ggtggcacga tgggacacac agatcgaagg tttcttttcc
    15361 gtaacgacgg cggtgctccc gcttcgccaa tgcaatgagg caatgatcgc ccgcttattc
    15421 ggtgcgtcca atttcgatga ggcatttccg aagttggccg ccgtacgtcg gttggccgaa
    15481 gctattcctc cgggagatcg ccccggttat gaccaaactt cggccggtcg atggcaggcc
    15541 caggttgaaa tgtcggcgcg actcatggag gctctgcgcg ccgttcgcca tattccgcct
    15601 tcgacacggg acacgcctgt gccaggcaaa tgtagtttga tgggcacctt cgagcagatg
    15661 ggaccagcga agttggaaga atcgcgccga ttttgggaaa ccatggccca gtgcgtccac
    15721 atcgggttgg atcgaattcg caagcgagaa cgcctttgtg gggtagccat cgttaagcga
    15781 tttgcaccgc attttctagc ccaaaagctc aaaatcgacg aacgtttgtt gcgtacgccc
    15841 gacacagcta cggtggcggc gacccggtgg ctgaaggaag catgcgaccg cggctatgaa
    15901 cttgatcccg aggcaatccg tgaacgttat gacgtgtgga gtggccaatg gctgttctgg
    15961 acaagaaaag accaaggcca ggacgaagac gaggacgcgg tcccggacga tgtgtgggag
    16021 gaaatccaga aagcaagaaa agataagtcg ctcggtccac cgccgaccta ttacgcgatt
    16081 ttgatgatgg acggcgactg gatgggacgt tggttgtctg gggagatggc accaccgatc
    16141 gaaaacgtgt tacaccccga gacgctatcc tatttccggc agttgaacga cccaggggcg
    16201 gaagcggctt tgagggcacc gcgtcccttg ggcttggtgg accacgcagt gatgagtcaa
    16261 gccctgacca attttgcgct tttcgtggtg ccgaagattg ttaaagagca tcacgggacg
    16321 ctgatctacg caggtggcga tgacgtcctg gccatgctac cggcctcgac agcggtggcc
    16381 tgcgcccgca ccttgcggct tgcctttagc ggtgacccac gggtcaacgg tggtgcccgg
    16441 ccaggttact acaggctggg gaaccgcgaa ttgttgatga tgggcccaag ggcaactagc
    16501 tcgtgcggaa tagccgtaat ccattacaaa gaagacctgc gatttgccct tcaacaggcc
    16561 cgacgggccg agaaggccgc gaaacgtgcc ggccgcaacc tgcttcagat tgccatctgt
    16621 cgccggtcgg gcgaacacac ctcggtcctt tgcccctggg agttcgtacc cactgtggaa
    16681 cgctgggttc aagccttttt gaggggcgcc tcggatggct ggacttaccg gttgcgtgcg
    16741 gagctgaaga cccttgctgc ggtatctcgg gaagctgtgc gggccgagtt gtcacgccta
    16801 gttgaacgtt ctgaacaaag cacgaaggac ctgctgagcg aaagcgaaaa tatctcggcc
    16861 ggccaaatgc ttgtcaggca gttcgacgat tatttgtcac agcttttggc gccgccgcgt
    16921 agcctcgatg aggagcaggc tatggagggc ttcgtccacc tttgccaagc ggcgtcattt
    16981 ttggcccggg ggagaaccga gtgatgaacc aatcgatttt atctgccaaa actcggctgg
    17041 tggccttgcg actcgatccg ctggacgtgc tttttttccg cgatggccgg ccgtttcacg
    17101 aggcctcacg cggggagtca gggttgcccc gacctcagac gctggcgggg gccctacgaa
    17161 ctcatctctt acgtcgcgca gggtgtgatg gggacggttt ccgtaaattg ggggagtacc
    17221 tgcgggctgg ccaatctctg gagcaggcaa tcacatccgt ctgtggcgcc ggctggattg
    17281 cgagggtcga agtccgtggt ccctggcttt gcgaagaggt caacggcaca cttgaaattc
    17341 tggtctcgat ccctgcaaac gtgcaagaga tcgaaggcgg tatggaaaca agctcggtaa
    17401 gcgagtccac caccgccgag aaaaatgtcc gatctggggt acggggtaat agcgaccggc
    17461 gttgggtacg atttgaccca ctggagcccg agctggatct gcccggatgg aagccgccag
    17521 tagcaggaat gcgcccttta tggccgcgcc tgcatgcccg gagtcagcga aggtccggct
    17581 accttagacc tgctgggttg gcaaagtttc tggccggcga ggaccctccg ccagaaaccg
    17641 tcgtctcgcc cgaggacctg tacggcttcg accagcgtac aggcaccgca atccaaccgg
    17701 accagttaac aattgaggaa catctgttct acgccgtcag tctgttggcc ttgcgacctg
    17761 gggtcgcttt ttatgccgaa gtgaatctgc cgtccgacgc accatcggag attttgcgcg
    17821 gggaaacact gctggatttc ggcggcgaag ggcacaaggt tctcgcaaga gaagtggcac
    17881 catttatgtg gcccgatcct ccggacgcat cgaacggtcg ggtgttatta ctgcttacga
    17941 cccccgttgt gttcagtaag cagtggtgtc ctcgtttgcc cgcgggaact cgattggtcg
    18001 cggccgctgt gaccgggtac gaggcctttt ccggctggga tttggcccgg ggcggtccaa
    18061 agcctactcg gttcgccgtc cccgccggaa gtgtctattt tctggagact tccgaccaac
    18121 gagtgttaat cagctctctg gccgagtcgg atgaagatcg tcaattgggc ttcggctgtg
    18181 ttgtacaagg agtttggaac tatgtctaat tcacttaatg gccgcagtgc agccaccttg
    18241 ttcctacacg cccaaacggg gctgcatccg ggcacgggca cagcgcttgg tgtggtcgat
    18301 ttaccggtac agcgtgagcg gcacacgcag tggcccacca ttgccgcctc ggccctcaag
    18361 gggattctgc gggatgtctg ccgcgaaaag gcgaaggctg atttcccaaa tggcgaaaca
    18421 ctctcgccac gtcgctgggc taacgaaaga caccaaaagc tggtggcagc ttttggcccg
    18481 ggcattccgg aggaaagctc tgagcacgcc ggcgccgtcg ctttgactga tgcgcgcatc
    18541 ctggcctttc cggtccgctc gctccgcggg gtatttgcct gggtaacttg cccagccgcc
    18601 gtgcaacggc tcaaccgcga cctggccttg acgggacttc aaccgctcga tgatattccc
    18661 gccgtgttgg acaacgagat cgcctgtaca tccagctcgc cgctcctggt gagccgcaac
    18721 ccggataagg tcgtgctgga ggaattcgat ttcaatcgca ttcaagctac atgcgatcag
    18781 ttggccgagt ggatggccca tcatgccgta gccgacgccg gcacccagag tcgtcttcgc
    18841 tggcacctgg ccgtcctctc agacaacgat ttttcgtact tcgtgcggca tgcaaccgag
    18901 atcgtcgccc gcattgcctt agactatgag cgaaaaacag ttaaggaggg tgccttattc
    18961 tacgaggagt tcttgcctgc cgagacgatc ttttacaccc ttatactggc cagtgaaagc
    19021 cgcaagccgg atgtgaaact ctcggctgcc gaaatcctcg agttcgtccg gcggtgcgtc
    19081 gaggccgccg gcgtgattca gattggtggc gacgaaacca ctggtaaggg actgtgtacc
    19141 gtccgttggc ttcaaaacga ggaggcatga tccatgagtc atggtacacg agatcgcttg
    19201 acgatcgacc aacagcgggc ccgacacgcc tggcgggccg tggagagtct gccagtggac
    19261 tccgaggaag ccgacgacta tgcccgcgaa gccaagaagc ttcccgtgcg aatcatcact
    19321 tccgggctag gccaagcctt ggcgtttgtt aacgccaaga aagccaaaaa gcagggcctg
    19381 gccaaactcg aagagcacct gaccgactgg gccaagcagc gtctggaggt agcctttcct
    19441 gataataaga cgttgctcga tgtcatcgta cacaacgata gcgatttcct tcggcgggcc
    19501 accgaagaaa tgttagccta tctcgtgtgg cttaatcgct ttactgaggc caaggggtta
    19561 cccaaaaaag gggagcagga ctgatggcgc caaccggaat tccgttgccc gaaagcgttg
    19621 tcaagctaat acaatctgtg ccgctaaacg agcggcatcc tggtcttcag cttgacaagt
    19681 acatcgaggc tcccgcttcg caggagcaac aaaaggaatc gcttaaatca gtgctcgagg
    19741 cggctaccga cgagcacctg cttcaagagg taactcggcg acgtgaggcc atcctccagc
    19801 agctcggggc tgtgcggttc actgcgaaga cggccggtcc aatgaccctt catctcgccc
    19861 gggcttccgc cctggaaaat gccggcatct gtctgcattc gctctatggg tttgtgtatt
    19921 ttccgggctc cggacttaaa gggatggcac gggcttatgc cgaaacggtt tggctaccgt
    19981 tgcaatccga tcagcaaaag gcgtggcggc agatcgaaga tgtttttggc tgggcatcga
    20041 accccactcg ccggcaacag atcagcgacc cgaaccaccc cgcccaagtt cgccgggtga
    20101 acgatagcga tccaaaatca agcgaaatct cggcctccgc ggggaacatc gtctttcacg
    20161 acgcttggcc agttcgctgg cccaagctcc aattggacat cgtcaataac catcacaccg
    20221 agtattaccg caacgagcag gctgccccag gcgactggga ggacccggag atggtgtctt
    20281 ttctggcgat tgggccaggc gaagagttta acttcgcgct ctccaagcgg cggtctgatg
    20341 tggccgacaa gttgctatac cttgcggcgc tttggttggc cggtgccttg acgtacgagg
    20401 gccttggagc caagacagcg gccggctatg gcgccttcat tgtttctagc ccgcctgtgc
    20461 ctgcgcccaa gccaccggcc cgagaagtct tcgaggcaac gttggaactg gttacgcccg
    20521 cctttctcgc cggaccaaat cagaacgccg ataattgcga cttgcggccg gccacactcc
    20581 gcggcctttt gcgctggtgg tggcgcacaa tgcacgccgg ctatgtggac ctaccgacgc
    20641 tgcaccggct ggaagctgcg gtctggggcg acaccaattg cggcggcccc gtccgtatta
    20701 cggtccagcg cgaagacccc agctcgccgt ctcccgtgcc cgtaccgggc aagtcgatcc
    20761 ggaaagataa aaacaatcgg gatgtgcttc gaccagacca agagttcatt cggcagcaaa
    20821 aacttgccct gccgcctgac caaaaaacga cccacggttt cctttacctc tcctacggga
    20881 tggatgagat gccagcgggt aagccggaag agcgcagacg ccggtttatt atgccccctg
    20941 gggcgaagtg gcgggtgcgc ctgatggctc gtgacggctt ctatcggccc gttggcgcga
    21001 gtgcctccgc caaacctgtg cggatccccg cggccgtgat tctcgagcag gctaaggcgg
    21061 cgctttggct tttatgtcac tacggtggtg tgggttctaa aggccgcaat ggatttggtt
    21121 ccttggtatt acttgacgac ggagacacgc agaacaaacg aggcgaactc gagcgggccg
    21181 cggcggagtt tcggcacata tgtggggtta acgagcaaat agagccacat tgccaaatcc
    21241 ctgctcttag gacagcgcta cccgaggtgt tggtttctac cccgtggaaa aaccactggt
    21301 ttgtactcga ccagctaggc tatagccttc agtcctttgc ccaaaaatac aagcggaagt
    21361 ggatcaagga ggctttgggc cttccgcgga ggattggcat ttcggatgac gacggttcgc
    21421 aaagtaaaca ttatcagcgc gaattggatc agaagacaaa gcaaccagtc gtctggctag
    21481 gccagaagca cccataccgg ggcaaccgaa aaccaaagga catgcgccac gccgccccgg
    21541 tccatttcca cattgcccgc agcgccgacg gaacctacca aatccgcgtc atcgcattcc
    21601 cttgtgcggt ccttccggat tggaaaacca gcaacgatgt gctccaaaaa ctccttgctc
    21661 accttcgcga ggacctgcag aaacgatgtg atgagaactc ccgggattca acactggtgt
    21721 ctcctgctat tgtgtctcgc gcgctcccgc gtattcctga gaagcggcct gcgggaacgc
    21781 cggtgcaggt gaagatcctg gctgaacgcc ccaagggtgg ttacgaggtc caggaggagg
    21841 gaaaacccca gggcacgttg accgtgggca ccccgcctac cgaacgccct gaagtcggcg
    21901 acatcgtttc tgtcgaggtc cacaatgacg atccgcgccg accgcaatac cgttggccgc
    21961 aaaatcgacc tcccatgcag tccgacaagt atccttccag ccgtcgctag ccgccctgag
    22021 agggcaatcg tcaaggccga ttgtcaatcg cgagtgccta cgccaagagc gttacttccc
    22081 agcagccggc cccggttacg cgatgttcgc cgatccccag gcggccgccc caaacaagcc
    22141 acggcagcag gcacaacggc acgcgaccaa agaccgcccc gccggtgacc cccacaagat
    22201 cataacgctg cctctggcga gtgctgtgat ggcgttggcg gaaaggctgc caaaaggccg
    22261 cctgggtggg aacggcctct gcgcacttct tcagccccgc aaaatccacg cgaccatcta
    22321 gccccttgcc gaaggccgca aaagcccgcc ccaccgtccg caagcacccc cgcaccaatt
    22381 gtcctaaagt gggtttcagc accgcccggg ccttgccgcc ttcaaccggc tcttctttca
    22441 gaaaaagcgg agtgataagg tccaagcgaa ctgccggcac atagccacga cagtcctccg
    22501 gcgacgcagg caagtgctcg ggctcgatgc attgcaaggc gcccggggca tccttttcgg
    22561 tcagatatgc cgaaagccga aagccaaccc ggtcccttcc aagctcaaac ccccgatctg
    22621 actccaacaa gacacggttg atcacgtcgg cggctgccgc cgcccgctcg cccaaaaatg
    22681 tcgcacacaa cgtcatgcgg tcgccaggcc gaccgtactc cggcacagga aaaggcggac
    22741 gaatcgtgat cgcccgctgc ccatcggcca tgcccgaggg cgccccctcc gacagtgttc
    22801 cctccgcctc aaatgtgctc ccataggcac agtcccccag atattggcaa cctcggcagg
    22861 tcttatatcg atcatcgcgt tcaagcggcg gccagcggca cacggcctcc ttgaccgccc
    22921 aagctagtgc cccgcgaatg gctggtccca gccacggccg gagtcgcccc tcgcctatca
    22981 attccattgt ccacaaccgg gttagaacaa gcacgcccac accccgatcc ccaccactta
    23041 ggccagccga gtcaatatcc ccaattccac cggcagcttc tccatctcct ctgccgtcaa
    23101 aatcgctcct tcgtccaatc ggcatttaat acgcagaacc aaagcgcggt cgcccggctg
    23161 catctccgca cggaaacgtt gaacactcac gggcataccc aatcgtttgc tgagaaactc
    23221 ggcggcaccc acatgtccca ccgccgagat aaaaccctcg gataagagtt cgactgcttc
    23281 cgccaccgaa atcgggcctt caaaccgcca atcgccgtag tcagacagca ccggtgcatt
    23341 caacacatac accttgccct tcactcgatg acactcggcc attagccact cctcccttgc
    23401 cgtaggtgta tcatgatagc tcagaaagag aagatcgaaa aggttatcgc gtggaaagcc
    23461 aataccgcca gggcttcttc gccccggtca gcccgcgctg gcagcggtgc cgttgtgtgg
    23521 ctggctcctc agcggcctgc cgccattgct ctgccgccac ctgttggtcc tgcgacacgg
    23581 cttttagccg accgacgtcg cggtgtcatc cctggtgttg gcctcttgcc tgcgctaact
    23641 ctgcagacaa acgctcgatc gtttcttgta gcttcgcccg ttcccgccta agctcctcgt
    23701 tctgttgctg caactctctc aactgcgctt gcgtccgttc ccacgccgcc agggcctcat
    23761 ctctttcctt gatggcctcc gtggccttga tttcctcccg cgaacggaag taattagggt
    23821 ctttcagcca ttcattcaac gccgctaccg cagcagcccg ttggccatcg gtctgggccg
    23881 tcgtttcgac caaaaacttc acgcagtacc gaccgtcgga atacacgccc acgatccctt
    23941 tggcggaaac caagaaacgg gtccgctcgg ataggtccgg gttaaagtag aatgtctcac
    24001 attgcacgac aaaaggcact tcctcggtga cgcgccgcat gaacgcctcg atctccggat
    24061 gttgcccagg ccagccagcc cgctccagcc gtggcggtct tttcgttggg gcgagcggcg
    24121 gcacaacaag atccttgacg ctatgaaagc gctccttgaa agtctcgtaa aaaatctggc
    24181 ccgcgggcga cagctccagg tactcggtat catcgatctt gacccgctcg accagactct
    24241 cataccgttc gtcccattcg tcttgataac gcgacgccgg aagaagttcg tgcgtatctg
    24301 acgccaattc tgacagaagg ccactggcct tcatccacaa ttgaaaatcc aaggcgaccg
    24361 gcagtggggg aaacgcgatg acttcgctaa atagctcatg cttgtaatac accgtgaccc
    24421 ccagagcttg ccccaagacc acagcgatgg cgatttgcgc tttgtagccg cccgtggcat
    24481 tgatcgcgca ggcgaggggc gaatgacgtc gcaccacggt cgctatttcc cgaaccagat
    24541 tccgcaaccc tttactgcga aagcgcttag gctcatcgtc ctgaaggtca gccacttcga
    24601 tgagctcaac cggctgatgg tcaacggccc ggtaatattc tttaagaatt tgggagattt
    24661 gtcgccctcg ttcggtctcc gaatggagaa aatacagacc gcactttgga tcgacatatc
    24721 ctcgctcgat cattccggcg atagagttaa tttccgcacc gcagagacga tccgtaggtg
    24781 gcagtcgcct gagcgcctgg gccacccccc gcgtgtcccc agaaatatag gcctcaacca
    24841 actgcctatg ctgcggggcc aatgagccgc cctcgaactg cgatttcagt ctctcgagat
    24901 tcggttgaac gagacttgtg cccacagtgc agatcagaat tgttttgggt gtcatcttcc
    24961 acctgcgctt actttgtcaa gacgaccgga ctaaacctca taggtcaagt cgaagatccc
    25021 tcgtaggggc aggcccctgt gcctgcgata tgcgttccca tgaccttgtc aacggccgag
    25081 tggcaaacgg aagctttccc cagccgctcg agtggcatta agttaacaac ttatcaagaa
    25141 ggtcaaaagg gcttccccgc gcagtaggaa aaacgattcg gattttttat tactgacgat
    25201 ttttcctccg actaaatcgc tcgaaatact cgtacggacc ccggtttatt tgtttggaca
    25261 gatcttgtcc aacttcgccc ccagaaaggg ttgacaacag cgttcggctc gccggatcgg
    25321 ccatgatccg gcccccgttc cctaagcccg ccggcaaaac gcatggtctt cggcgaccgg
    25381 gcgagactca gcgttcgcgg tgacccaatc actagacggc ggggacgccg tccctctcgg
    25441 ggctattcaa tgattgggca gttgtcgcca cgtgtgccag gggctgtaag caatgatttg
    25501 cccgtccgtt agtggagcaa accggccaga tgcagctagt agctattgcc cggcctgagt
    25561 acctcccccg cctggccgcc gaacgtccct gcgaaccaat ctttgggatt cagctgcgca
    25621 tccatcctgg tcgtaaacac cactgtccat ggcttgttct ccgcggtgga tagcgggttt
    25681 acaatcactc cctgccctag agcgaagccc gttgttattt tcaggtccgt tttgggagaa
    25741 cgcttctgct gacgcgctac gcttgtggcg aggacctatt taaagcctat cccataatct
    25801 cccctcgccc gttcagcggg agaggggtcg gggtgagggt ggctcgaatc gctgccggtt
    25861 ttctcaatca attccgacgt tcgcttcccc tcaccccaac cctctcccgg agggagaggg
    25921 ggactttttg ggataaacgc taatgagaaa gattagacaa agga
//
LOCUS       DAOSUT010000048        25932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1238_length_25932_cov_9.911079, whole genome shotgun sequence.
ACCESSION   DAOSUT010000048 DAOSUT010000000
VERSION     DAOSUT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 25932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25932
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_25932_cov_9.911079"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLD05_06115"
     CDS             complement(<1..1168)
                     /locus_tag="PLD05_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77046.1"
                     /translation="MSENLERLKQLLAEMFQLDQAELDFGIYRIMNAKREEITRFLDR
                     DLLPQVREVLGEYEKDSRAAIAAELERAREQAKSLGFEDPAQAPRVKELQARYNAAFD
                     LEAAEQEIFSHLYNFFRRYYHEGDFISLRRYKEGVYAIPYEGEEVKLYWANHDQYYIK
                     TSEYLRDYTFKLPSGRRVHFKLVQADTEKDNNRATNGNERRFILGHGDGGTGGQGDVW
                     DIVNDELVLYFRFVPDGAKRKQAELNAEAVRRITDIISGSPSLRDAWSPLLEKRPTEK
                     NPDRTLLEKHLADYTARNTFDYFIHKDLGGFLRRELDFYIKNEVMHLDDIENETAPRV
                     EQYLSKIKAIRRIAHKIIDFLAQIENFQKKLWLKKKFVVETQYCITLDRIFAIED"
     gene            complement(1180..4080)
                     /locus_tag="PLD05_06120"
     CDS             complement(1180..4080)
                     /locus_tag="PLD05_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOP77047.1"
                     /translation="MSTAYHAKYFAYELTRIGGTGMERLSRSLFDACVDLNPHQIEAA
                     LFALRSPISKGVLLADEVGLGKTIEAGLVLCQLWAERRRRLLVICPASIRKQWALELT
                     EKFNLPTVVIDAKEYRERQGRGMPNPFEADEVVVTSMHFASAHASDIRSAQWDLVVID
                     EAHKLRNAYRDSNRMGQNIRWALEDRRKVLLTATPLQNSLLELYGISTIIDDHIFGDL
                     PSFRSQFVNAGGNVEELRNRLRTFSIRTLRRQVVEYIQYTERRLITRPFKPTEQEHKL
                     YEAVSAFLQRTDTYALPYQQRHLTALIVRKLLASSPHAVAATLEAMRDRLIAIRDKLP
                     VPEDFAERLIADEEMEHELLDEMTDGLLQRSGDEDAEKRPQVTEAEQAEPVIDRQKLL
                     AEIEELDRYAQWARSIGIDTKTRSLIKALEIGFEKMTEMGAAQRAVIFTESRRTQAYL
                     KDFLEANGYAGRVITFNGSNNEPETTAIYERWVEANKETGRVTGSRSVDVRTAIIEHF
                     RDHGQILIATEAAAEGVNLQFCSLVVNFDLPWNPQRIEQRIGRCHRYGQKHDVVVINF
                     LNEKNEADRRVYELLQEKFSLFSGVFGASDEVLGSIESGVDFERKVLDIYQQCRTPDE
                     IEQAFNALRAELDEKIRSKLAETRQKLFEHFDEDVHAHLRLNLEGARQQLDRVGMMFW
                     RLTRFVLDGRATFDEANLAFDLKTPPHDGIQPGYYRLISKDHENVTGAFLYRLSHPLG
                     EYVVETGKTLATPVAHVTFDISGRPTRISLIEALKGRSGWLHLQRLTIDSFEREEYLL
                     FSGIDDDGRSLDQETLEKLFHCEATAEPLSSLPSEVEARLAAEAERHAKATISRSLET
                     NNRHFQEARERLEKWADDMVLAAEKELKDTKERIKALSRQARLATTTEEQYALQKQIQ
                     ELEKQKRRQRQRIFDVEDQIMAKRDQLIDNLEKRMQQRTSVEPLFTIRWSVV"
     gene            complement(4146..4451)
                     /locus_tag="PLD05_06125"
     CDS             complement(4146..4451)
                     /locus_tag="PLD05_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOP77048.1"
                     /translation="MKNEGMPGLIRELRQRLNLTQEQLAEKVGVASSTVNHGQNGKRV
                     PLPFLVKRLVELKYEFGRKLEESERYKAVKALARKTDHLLFLTAIRTEGEFRSPLWC"
     gene            complement(5452..5525)
                     /locus_tag="PLD05_06130"
     tRNA            complement(5452..5525)
                     /locus_tag="PLD05_06130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5489..5491),aa:Arg,seq:ccg)
     gene            5932..7323
                     /locus_tag="PLD05_06135"
     CDS             5932..7323
                     /locus_tag="PLD05_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HOP77049.1"
                     /translation="MLKRLSSSLLENFKTKPQYPTAPIPTDGLPPGVPYIVGNEAAER
                     FSFYGMRAILTIFMTQYLLDAHGRLAPMSESEAKFYFHLFNAGVYFFPIVGAILADIF
                     WGKYSTIVGLSLVYCLGHVALAWDETRLGLFVGLTLIAVGSGGIKSCVSAHVGDQFAS
                     RNSHRMGEVFAWFYLAINLGAASSTLLTPWLLVVAGPGLAFAVPGILMALATAVFFAG
                     RAKFAHIPPSGKKFFYELISPEARSTILRLIPLFLCVAMFWALFDQSGSAWVLQAEKM
                     NRRWLGIQWESSQIQAVNPLLVMIMVPLSTYVIYPLVNRVWQFTLLRRVSVGMYVAAC
                     SFCVTAWIEHTIAAGARPSIGWQVAAYVVITAAEILISVTCLEFAYTQAPRTMKSVIM
                     GLYLLSVSLGNSFTAVVNKFIELTGGDRPGGWAQGPGYYWFFVLAMALTGVVFTIVSR
                     FYREKTYLQASET"
     gene            7649..7987
                     /gene="rpsJ"
                     /locus_tag="PLD05_06140"
     CDS             7649..7987
                     /gene="rpsJ"
                     /locus_tag="PLD05_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOP77050.1"
                     /translation="MSATINEIIRIRMESYDHAVLDQSAREIVDTVKRTHSKVHGPIP
                     LPTRIERYTVLSSPHVDKKARQQFEIRTHKRLIDILEVTPATIEALNKLTLPAGVDIK
                     IKATSRGRSR"
     gene            8148..8837
                     /gene="rplC"
                     /locus_tag="PLD05_06145"
     CDS             8148..8837
                     /gene="rplC"
                     /locus_tag="PLD05_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOP77051.1"
                     /translation="MAIGLLGRKVGMTQIFDDAGNVIPVTVIQAGPCHILQIRTPQRD
                     GYEAIQLGYLDKPRHRANRAERGHVARLESKRQKLLAAAGIQAPPKANCEPKRFIKEF
                     RTSIEGFSVGQVLTVDLFKDVAAVDVTGTTKGRGFTGVMKRHGFSGQRASHGVKNVHR
                     HPGCTGCNTSPGRVVPGRRMAGHYGHEKCTIRNLKLVRIDSERHLLLVKGAVPGPVGG
                     LIIIRPTNKVG"
     gene            8913..9551
                     /gene="rplD"
                     /locus_tag="PLD05_06150"
     CDS             8913..9551
                     /gene="rplD"
                     /locus_tag="PLD05_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOP77052.1"
                     /translation="MVSIPVYNREGQQVDTYEIDPAELAPRINKQLLHDAVVMYRANL
                     RLGTAKAKSRSEVAGSTRKLYRQKGTGRARAGMRRTPIRRGGGRAHPKRPQDWSYRLP
                     KKALRLATRMALASRIADNEVILIDELKFEAPKTREAAGILNKLGLAGMRLLVTLDNY
                     DPIAYKSLRNIDKVETLPVSDINAYEVLRPKRILMTRAALDMFRAKVVAGTA"
     gene            9571..9885
                     /gene="rplW"
                     /locus_tag="PLD05_06155"
     CDS             9571..9885
                     /gene="rplW"
                     /locus_tag="PLD05_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOP77053.1"
                     /translation="MLRNTPQPAVSGLEPHQVIIRPLITEKSHHKAERYNQYAFEVSP
                     LATKADIKKAVEELFNVKVLRVNIQNRRGKERRYRWRKAYTKAWKKAIVKLDPEYRIN
                     LY"
     gene            9920..10777
                     /gene="rplB"
                     /locus_tag="PLD05_06160"
     CDS             9920..10777
                     /gene="rplB"
                     /locus_tag="PLD05_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOP77054.1"
                     /translation="MGIRAYKPTSPGRRGATVSDFAELTPGAEPEKSLLVPLKKKGGR
                     NNQGIITCRHRGGGHKRLYRIIDFKRNKDGIPARVDSIQYDPNRSARIALLKYADGEK
                     RYIIAPDGLKPGDTVMSGPGAPPNIGNALPLSEIPLGMQVHNIELIPGKGGVMCRSAG
                     CAATLVARDGDWAQLTLPSGEIRRVSARCRATIGVVSNADHEGIVLGKAGRKRWLGRR
                     PHVRGTAMNPIDHPHGGGEGRTKGGRHPVSPTGKPAKGGYTRKRGKPSNKAIVRRRRS
                     VRYGQLPIR"
     gene            10829..11098
                     /gene="rpsS"
                     /locus_tag="PLD05_06165"
     CDS             10829..11098
                     /gene="rpsS"
                     /locus_tag="PLD05_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOP77055.1"
                     /translation="MGRSLKKGPFVDPNVFAKVAKLNEAGKKEPIKTWARACTIVPEF
                     VGHTFLVHNGKTHVKVFVTEDMVGHRLGEFAPTRIFRGHGGRGKK"
     gene            11189..11554
                     /gene="rplV"
                     /locus_tag="PLD05_06170"
     CDS             11189..11554
                     /gene="rplV"
                     /locus_tag="PLD05_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOP77056.1"
                     /translation="MSISNMSRMYRALHRYARISPRKARLVADVVRGKQVDEALAILK
                     TMPHRAARMIEKVVSSALANSADLQDPFPRELHVIRIWVDEGPRLKRLRPLSRGMATI
                     IQRRMCHIGVILGPREYLK"
     gene            11585..12322
                     /gene="rpsC"
                     /locus_tag="PLD05_06175"
     CDS             11585..12322
                     /gene="rpsC"
                     /locus_tag="PLD05_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOP77057.1"
                     /translation="MGQKVNPIGFRTGITLDWKSRWIAPKREFADLLLEDYKIRRYIL
                     ERKEPSAEKGGRGERRAYPMISKIEIERTRDEVKVIIFTARPGVLIGSKGGKIDQLQQ
                     ELQELTGRRINLKIEEITRPELVAQLVAESIAEELERRVSFRRAMKKAMETAMAQGAR
                     GIKVQLSGRLGGAEMSRREKQIMGSVPLSTLRANIDYGFAEATIAQGQIGVQVWINLG
                     MFSEVDSHGVDAKTGQTSKKSKRAYKG"
     gene            12261..12677
                     /gene="rplP"
                     /locus_tag="PLD05_06180"
     CDS             12261..12677
                     /gene="rplP"
                     /locus_tag="PLD05_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOP77058.1"
                     /translation="MALMPKRVKHRKSQRGRIRGNATRGNRVVFGEYGLQAVQGGWIP
                     AATIEAGRIAAQQYLRSEGRLYIRIFPHKPITSIPLETRMGKGKGEPEYWAAVVKPGT
                     ILFELGGISEEAARLCFSRLAHKMPVKVRFVRRQTM"
     gene            12753..12962
                     /gene="rpmC"
                     /locus_tag="PLD05_06185"
     CDS             12753..12962
                     /gene="rpmC"
                     /locus_tag="PLD05_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOP77059.1"
                     /translation="MKARELREMSTEQLEALLRETTEALFRLRIQKQTERLASPMEPR
                     MKRRLIARIKTILNERARQEASAAK"
     gene            13030..13398
                     /gene="rpsQ"
                     /locus_tag="PLD05_06190"
     CDS             13030..13398
                     /gene="rpsQ"
                     /locus_tag="PLD05_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOP77060.1"
                     /translation="MPRRREIGIVTKANTPQLRRVEVIRLVQHPRYKKYVRHRTICYV
                     HDENNESQVGDRVEIMESRPLSRLKRWRLVRVVERGRAELLQKRRVVEEELLEASRTG
                     TEAREASPASQETQQSDQQG"
     gene            13462..13830
                     /gene="rplN"
                     /locus_tag="PLD05_06195"
     CDS             13462..13830
                     /gene="rplN"
                     /locus_tag="PLD05_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOP77061.1"
                     /translation="MIQMQTRLDVADNTGVKELMCIKVLGGSRRRYATLGDIVVCSVK
                     GVIPGSTFQKGAVVRAVIVRTKKPKRRPDGSYVRFDSNAAVIVDNDGNPRGTRIFGAV
                     ARELRERNFMKIVSLASEVV"
     gene            13830..14183
                     /gene="rplX"
                     /locus_tag="PLD05_06200"
     CDS             13830..14183
                     /gene="rplX"
                     /locus_tag="PLD05_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOP77062.1"
                     /translation="MWIRIDDTVEVISGDDRGVRGRVLRVDRKQGRVVVEGVNIVLKH
                     VRRSRQARQGGRLSKERPIDISNVMLVCPSCKQRTRVGARFLADGSKVRYCKKCDANI
                     GQISRPKSRSQTANA"
     gene            14204..14749
                     /gene="rplE"
                     /locus_tag="PLD05_06205"
     CDS             14204..14749
                     /gene="rplE"
                     /locus_tag="PLD05_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOP77063.1"
                     /translation="MEPRLLEKYRKEVIPILMEKTGRTNRLSLPRLEKIVINMGVGEA
                     KKEKKYLDEAVDALTNIAGQKAVITRARKSVAGFQVRQGMAIGCKVTLRGRRMYEFLD
                     RLISIALPRVRDFRGLNPRAFDGHGNYALGLTEQMVFPELNPDKYSRNQGMDICIVTT
                     ARNDREALELLKALGMPFRAE"
     gene            14790..14975
                     /locus_tag="PLD05_06210"
     CDS             14790..14975
                     /locus_tag="PLD05_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOP77064.1"
                     /translation="MASKSKIAKFRKKPKFSTRFRNRCRICGRPRAVYRKFGICRICF
                     RKLADNGMIPGVVKASW"
     gene            14992..15387
                     /gene="rpsH"
                     /locus_tag="PLD05_06215"
     CDS             14992..15387
                     /gene="rpsH"
                     /locus_tag="PLD05_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOP77065.1"
                     /translation="MMTDPIADVLTRIRNAVRVERPHVDVPTSNLKRGLADVLKREGY
                     VWDWEEIPSQPRAILRIYLKYGPNGEQVIRHIRRISKPGRRVYSRAADLKPVLNGFGI
                     RILSTSRGIMSDREARQKKVGGEILCEVW"
     gene            15434..15979
                     /gene="rplF"
                     /locus_tag="PLD05_06220"
     CDS             15434..15979
                     /gene="rplF"
                     /locus_tag="PLD05_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOP77066.1"
                     /translation="MSRIGKRPVPIPQGVTVTIEGRVIRVKGPKGELTFEHRPEVSVS
                     VDTARSAVVVERVQETRLARALHGLTRALVNNMVIGVTQGYEKRLEISGVGYLAAVQK
                     GVLQLRVGYANELQVPIPEGLEVTCPDQQHIVIRGVDKQKVGNFAAAVRALRKPDPYK
                     GKGIRYQGEHIRMKEGKVSAK"
     gene            16102..16464
                     /gene="rplR"
                     /locus_tag="PLD05_06225"
                     /pseudo
     CDS             16102..16464
                     /gene="rplR"
                     /locus_tag="PLD05_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            16611..17102
                     /gene="rpsE"
                     /locus_tag="PLD05_06230"
     CDS             16611..17102
                     /gene="rpsE"
                     /locus_tag="PLD05_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOP77067.1"
                     /translation="MASDLEPIRGQLIEKVLSVRRCAAVVKGGRRFSFTALVVVGDGM
                     GKVGWGYGKANEVPPAVAKAIKDGMRRMKTVTIVDGTIPHQVKGSFGAAEVVLVPAGP
                     GTGVIAGAAVRAVCEAAGIKNILTKSYGNTNPINLVKATMNALEQLRSKAEVERLRGT
                     SLS"
     gene            17099..17593
                     /gene="rplO"
                     /locus_tag="PLD05_06235"
     CDS             17099..17593
                     /gene="rplO"
                     /locus_tag="PLD05_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOP77068.1"
                     /translation="MNIDQVNRGVVTHKKRKRVGRGPGSGHGKTCGRGHKGARSRSGW
                     REKAGFEGGQMPLIRRVPKRGFNNARFALRIAEINLEVLEKAFADGDEVTPEILEQRG
                     LVKRVYDELKILGNGELTKKLSVSAHRFSRSAKEKIEKAGGKVIVLPGKTPVEEKKKA
                     VKGA"
     gene            17644..19005
                     /gene="secY"
                     /locus_tag="PLD05_06240"
     CDS             17644..19005
                     /gene="secY"
                     /locus_tag="PLD05_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOP77069.1"
                     /translation="MWEKIRVIFTIRELRQKILLTLLLLAIYRIGEQIPVPGINTEKM
                     TAIFRSQAAGGLRDLLDQVTVFAASRLDQATIFGLGIMPYISASIIFQLLASVYPPLE
                     KLKKEGPAGYKKINEYTRYATVGICIIQSWMYVAWLAAQGFVRPEYTYGEGQGVYFSW
                     FLTAVITMTAGTTFLMWLGEQIDEYGIGNGISLLIMAGILARMIPALYQELRPVFDEG
                     LELSGTAGKIGFDTLMILLVLFVSVVAGVVYITRGQRRILTQSAKHVRGRRVYGGARD
                     YLPLLVNQAGVMPIIFASSLLLFPLTFFQTLAHWYPNSSWLAAAANVFHRGDGFWYNA
                     MYIGLIYFFCYFWTAITFNPNEIAEQLKDNGSIIMGYRPGRRTAEYLEKVMVRITYVG
                     AGFLAAVAIIPTIVADWLGVNYQIAQFYGGTSLLIAVSVAMDLVQKIDSHIVMRNYKG
                     LLE"
     gene            19086..19649
                     /locus_tag="PLD05_06245"
     CDS             19086..19649
                     /locus_tag="PLD05_06245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOP77070.1"
                     /translation="MRLVFIGPPGAGKGTQAERIVSRYMIAHLSTGDMLRAARNAKTP
                     LGIQADAYMSRGELVPDDLIIDLIRERLAADDCQRGYLLDGFPRTIAQAEALDKMLGE
                     KKTPLDVVLELRVPEEELFRRLAGRGRADDKPEVIRQRLVAYREQTAPLLDYYQEKGL
                     LKTVDGLGTVDEVFERIRSILDEFAGA"
     gene            19703..20524
                     /gene="map"
                     /locus_tag="PLD05_06250"
     CDS             19703..20524
                     /gene="map"
                     /locus_tag="PLD05_06250"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOP77071.1"
                     /translation="MITLRSRSEILSMRRAGLVVWKALELVKSMVRPGLMTKEIDQAV
                     EDFFKRSGAQPLFKGVPGRVPFPAVTCISVNEEVVHGIPGDRQIKEGDIVSVDTGCRV
                     DGWCGDAAVTIPVGRPAPEVLKLVEVTRQTLDLAIELMGRSRLWSEVAAAMQRYVEKH
                     GFSVVEAFVGHGIGRSMHEEPQVPNFVNALFLKKGDFALQPGLVIAIEPMVNLGTKKV
                     RLCRDHWTQVTADGKPSAHFEHTVAMTEFGPYVLTGPLGPMDEKLGFDPDRHHFA"
     gene            20675..20788
                     /gene="rpmJ"
                     /locus_tag="PLD05_06255"
     CDS             20675..20788
                     /gene="rpmJ"
                     /locus_tag="PLD05_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOP77072.1"
                     /translation="MKVRASVKRLCSNCKVVRRQGRIYVICSNPRHKQRQG"
     gene            20844..21227
                     /gene="rpsM"
                     /locus_tag="PLD05_06260"
     CDS             20844..21227
                     /gene="rpsM"
                     /locus_tag="PLD05_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOP77073.1"
                     /translation="MPRLLGVDIPGNKPTVIALRYIYGVGPAIAKELCYKAGIDPHIR
                     ANQLREDELARLATLLDKDYTVEGQLRRQIAQNINRLKDINCYRGIRHRRGLPVRGQR
                     TRTNARTRKGPRKTVAGKKGVKDLR"
     gene            21293..21679
                     /gene="rpsK"
                     /locus_tag="PLD05_06265"
     CDS             21293..21679
                     /gene="rpsK"
                     /locus_tag="PLD05_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOP77074.1"
                     /translation="MAKIGPKRKKIRRHVTQAIVHIKTTFNNTMVTVTDLKGETLCWA
                     SAGTCGFKGSRKSTPYAGQMAAQQAAEKAIKMGVKEVEVKVKGPGAGRESAITALQTA
                     GLVVKSIEDVTPLPHNGCRPKKRRRV"
     gene            21765..22391
                     /gene="rpsD"
                     /locus_tag="PLD05_06270"
     CDS             21765..22391
                     /gene="rpsD"
                     /locus_tag="PLD05_06270"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOP77075.1"
                     /translation="MGRYTGPVCRLCRREGMKLFLKGTRCDTDKCAFERHKATPGMQG
                     VLRGKPTDYALHLREKQKLKRFYGIYERQFRRYFEMATRSPTNTGEVLMSLLERRLDN
                     IVYRLGFALSRAQARQLVAHGHILVNGRKVTIPSYLVSPGDVVSVKNRPRSLDAVQRN
                     LAERQPMVPDFLNLITGPVPEGHVLRNPTLEDVSLPIQPQLVIEFCSR"
     gene            22438..23430
                     /locus_tag="PLD05_06275"
     CDS             22438..23430
                     /locus_tag="PLD05_06275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOP77076.1"
                     /translation="MRVQWRGLELPSQVVCDRSTLTDRYGKFIAEPFERGFGVTIGNS
                     LRRILLSSLEGSAVTKVKIQGALHEITSLPGVVEDVTDIILNVKALVVRNHSSQPRTL
                     RINRCTKGVVTAADIEPDDQVTIINKDHVIATLTDDVPFVMEMVVENGRGYVPASEHT
                     PQISEIGVIPVDAMFSPVVKVRYEVEETRVGQKTNYDKLTMEIWTDGSITPEMALVEA
                     AKILRKHLNPFVQYTELGPKVFADSRSVLSQDSTLDSKLSVSLADLKFSTRTLTSLES
                     AGIHTVRDLVAMTEQQLLDLRNFGETSLAEVKDRLATLGLRLGMRTSPSPGGVV"
     gene            23505..24167
                     /locus_tag="PLD05_06280"
     CDS             23505..24167
                     /locus_tag="PLD05_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOP77077.1"
                     /translation="MRHRKKGRYLGRSPSHRRALLRNLASSLFLTEGDFLFEHEEPYV
                     KGRIITTLAKAKEVRPLVERCITIAKRVLPLLEEAKRMEPTAPRNSEAWRRWRQSDAW
                     YEWNRKMAPVVAARRRILRMLGNNKEAVRVLFEKIAPRFVDRPGGYTRIVRLAKRRVG
                     DAGVKAFIEFVGQNDRVKQRKTIRPEFSVETPKPSEPAPVKEATIDTPEAANVSQENP
                     SN"
     gene            24499..25410
                     /locus_tag="PLD05_06285"
     CDS             24499..25410
                     /locus_tag="PLD05_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77078.1"
                     /translation="MRLPTGDYQRIDWKTSHGMTTRVAAHFERMCALLVILGMASGCT
                     HLRMPASSPLVPAQMSSDSVALDIIFAHVPPSDDVLNQIWTELDEQFLPQETRKGLYQ
                     NGFRIGRCAGPVPQSLAEVMKLTDSGPAYSEVNTADAMTLTDDDTPTRRHLQLRTGCR
                     TEILASAVYEELPFLRREDGRLVGRTLPKAQGVFALTTRPGTYGGTRIRLVPEVHFGE
                     PRIQPIAKQGMIRWESQRDRIVLDDLIVETELHPGDLLVLTCLPNCPGSLGNRFFTVS
                     SQGKMQQKIIIIRLSQTQASSLFTANE"
     gene            complement(25565..>25932)
                     /locus_tag="PLD05_06290"
     CDS             complement(25565..>25932)
                     /locus_tag="PLD05_06290"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOP77079.1"
                     /translation="IQKALPEGYPLVMHGASSVPKEWVDRINAAGGALKQTSGVNEDD
                     YAKAAALGVCKINIDTDGRLVWCAVYRETFRDKPEDFDPRTPGKVFMAAYAEYIIHKN
                     KKLGSAGQLKEVAAFVRGG"
BASE COUNT         5716 a         6914 c         7745 g         5557 t
ORIGIN      
        1 cgtcctcgat ggcgaagatg cgatccagcg tgatgcagta ctgggtctcc accacaaact
       61 tcttcttgag ccagagcttc ttttgaaaat tctcgatctg ggccaaaaag tcgatgatct
      121 tgtgggcaat ccggcggatg gccttgatct tggacaggta ctgctccacg cggggagcgg
      181 tctcgttttc gatgtcgtcg aggtgcatca cctcgttttt gatgtagaag tccagctcgc
      241 ggcgcaggaa gccgcccagg tccttgtgga tgaagtagtc gaacgtgttc cgcgcggtgt
      301 agtccgcaag atgcttctcc aaaagcgtgc ggtcggggtt cttctcggta ggccgttttt
      361 cgagaagggg cgaccaggcg tcccggagac tgggcgaacc gctgatgatg tccgtgatgc
      421 gacgcaccgc ctcggcgttc agttcagcct gcttgcgctt tgccccatcg ggcacaaaac
      481 ggaaatagag caccagttcg tcattcacga tatcccagac gtccccctgt cccccagtgc
      541 cgccgtcccc gtgtcccaga ataaagcggc gttcgttgcc gtttgttgcc cggttgttgt
      601 ccttctctgt gtcggcctgg acgagcttga agtggacccg ccggcccgag ggaagcttga
      661 acgtgtagtc gcgcaagtac tcgctggtct tgatgtagta ctggtcgtgg ttggcccagt
      721 agagcttgac ctcttccccc tcgtagggga tggcgtagac gccctccttg taacggcgta
      781 gggagatgaa gtcgccctcg tggtagtacc ggcgaaagaa gttgtagagg tgcgagaaga
      841 tttcctgctc ggcggcctcg aggtcaaagg cagcattgta gcgtgcttgc agctctttca
      901 cgcgcggggc ctgggcgggg tcctcaaacc ccaggctctt cgcctgctcc cttgccctct
      961 caagctcagc cgcaatggcc gcccgtgagt ccttttcata ttcaccgagc acctcgcgta
     1021 cctgcggtag aaggtcccgg tcgagaaagc gggtgatctc ctcccgcttg gcgttcatga
     1081 tacggtagat gccgaagtcc agctcggcct ggtcgagctg gaacatctcc gccaaaagct
     1141 gtttcagtcg ctccaggttt tcggacatgt tcatcctcac tacacgacgc tccagcggat
     1201 cgtgaacagg ggttcgacgc tcgtccgctg ctgcattctc ttttccaaat tgtctatcaa
     1261 ttgatcgcgc ttggccatga tctggtcctc gacgtcgaag atgcgctggc gctgccggcg
     1321 tttctgcttt tcgagttcct gaatctgctt ttgcaaggca tattgctctt cggtagtcgt
     1381 ggccagtcgc gcttggcggc tgagcgcctt gatccgttcc ttggtgtcct tcagctcctt
     1441 ctcggccgcc aggaccatgt catcggccca tttctcgagc ctctcccgcg cttcctggaa
     1501 atgccggttg tttgtttcca gcgagcggct gattgtggcc ttggcgtggc gttcggcttc
     1561 cgcggccagt cgggcttcga cctcggacgg taaagaagaa agcggctcgg ccgtggcctc
     1621 gcagtggaat agtttctcga gggtctcctg atcgagagac ctgccatcgt cgtcgatgcc
     1681 ggaaaagagc aggtactcct cccgctcaaa ggagtcgatg gtgagtcgct ggaggtgcag
     1741 ccaaccggag cggcctttga gggcctcgat cagagagata cgcgtgggcc gcccgctgat
     1801 gtcgaacgtg acgtgggcga ccggagtggc cagcgttttg ccggtctcga ccacgtactc
     1861 gcccaagggg tgcgacagac gatagaggaa tgctccggtg acgttctcat ggtccttcga
     1921 gatcaggcgg taataccctg gctggatgcc atcgtgcgga ggcgttttga ggtcgaacgc
     1981 caggtttgcc tcgtcgaacg tcgcccgccc atccagaaca aaccgcgtga gccgccagaa
     2041 catcatccct acgcggtcga gctgctggcg tgcgccttcc aggttcagtc gaagatgggc
     2101 atggacatcc tcgtcgaagt gttcgaaaag cttctgccgg gtctcggcca gcttggaacg
     2161 aatcttctcg tcaagttcag cccgcaaggc attgaaagcc tgctcgatct cgtctggcgt
     2221 ccgacactgc tgatagatat ccagcacctt ccgctcgaag tcgacgccgg actcgatgct
     2281 gcccaggacc tcgtcggaag cgccgaacac gccgctaaag aggctgaact tctcctgtag
     2341 cagttcgtag acgcgccggt cggcctcgtt cttctcgttg aggaagttga tgacaacgac
     2401 gtcatgcttc tgcccgtacc gatggcagcg cccgatccgt tgctcgatcc gttggggatt
     2461 ccagggaagg tcgaagttta ccaccaggga gcagaattgg aggttcacac cctcggcggc
     2521 cgcttccgtt gcaatcagga tttgtccatg gtcgcggaag tgttcgatga tggccgtacg
     2581 gacatccact gaccgcgacc cggtcactcg accagtttcc ttgttggctt ccacccagcg
     2641 ctcgtagatg gcggttgttt ccggttcatt gttgctcccg ttaaacgtga tgacgcggcc
     2701 tgcgtacccg ttcgcttcca ggaagtcctt gagataggcc tgggtccgtc tcgactccgt
     2761 gaagatcacc gctctctggg ccgcccccat ctcggtcatc ttctcgaagc cgatttcaag
     2821 ggccttaatc agcgagcggg tcttcgtgtc gatcccgatg cttctcgccc actgggcgta
     2881 gcggtccagc tcctcgattt ccgcgaggag cttctggcgg tcgataacgg gttctgcctg
     2941 ttcggcttcg gtaacctgag ggcgcttttc cgcatcttcg tcgccagaac gctgcagaag
     3001 tccgtcggtc atctcatcca gcagctcatg ttccatttcc tcgtccgcga tgaggcgctc
     3061 ggcgaagtcc tcggggacgg gcagcttgtc acggatggca atcagtcggt cgcgcatggc
     3121 ttcgagcgtg gccgccaccg catgcggcga tgaggcgaga agcttgcgga cgatcaatgc
     3181 agtcaggtgt cgttgctgat agggaagcgc gtaggtgtcc gtgcgctgga gaaacgccga
     3241 caccgcctcg tacagtttgt gctcctgctc cgtgggcttg aaggggcgcg taatgagacg
     3301 gcgctctgtg tactggatgt actcgacgac ctgccgccgg agagtgcgaa tggagaacgt
     3361 ccgaagcctg ttccgcagtt cctccacgtt cccaccggcg ttcacgaact ggcttcggaa
     3421 ggacggaaga tctccgaaaa tgtgatcgtc gatgatggtc gagattccgt agagctccag
     3481 caaggagttc tgaagcggcg tggccgtcag gagcactttg cgccggtctt caagtgccca
     3541 ccggatgttc tggcccatac gattcgagtc ccggtaggcg ttgcgcagct tgtgagcctc
     3601 gtcgatgacc accaaatccc actgggcaga acgaatatcg gacgcgtgag cactggcaaa
     3661 gtgcatggac gtgaccacga cctcgtccgc ctcgaagggg ttgggcattc cccggccctg
     3721 tcgttcccgg tactccttcg cgtcgatgac gaccgttgga aggttgaact tctcggtcag
     3781 ctcaagcgcc cactgcttgc gaatggaggc ggggcagatg acgagaagcc ggcgccgtct
     3841 ttcggcccaa agctgacaaa gaaccagacc ggcctcgatg gtcttcccga gaccgacctc
     3901 gtcggcgaga aggacgccct tggagattgg ggatcggagg gcgaacaggg ccgcctcgat
     3961 ttggtgcgga ttcagatcca cgcacgcgtc gaacagggac cgggaaagac gctccatccc
     4021 cgtgccgccg atgcgggtca gctcatacgc aaagtacttc gcgtgatagg cggtgctcat
     4081 cactacgtct tccccgtcag tctcttgctc tcgttttcgt caatcctgta tctcatatta
     4141 ccagatcaac accagagggg agatcggaat tctccctcgg tgcggattgc cgtgagaaac
     4201 agcaaatgat cggttttgcg ggcgagggcc ttcaccgcct tgtaccgctc gctctcttcc
     4261 agcttcctgc cgaactcgta tttcaactcg accagtcgct tcacaagaaa cgggagaggc
     4321 acccgcttgc cgttctgccc atggttcacg gtgctggagg cgacaccaac cttctccgcg
     4381 agctgctcct gcgtgagatt cagccgttgt cgaagttcgc ggatcagccc cggcatgccc
     4441 tcattcttca tggtgatatc tcgcaccctc gtttgcgaac gcggcgaaga atatttatat
     4501 gcatatatgc cgccatgata gccctcatgc gacgcggctg tcaacgtttt gaatggcctc
     4561 agatggtccg agagactttg cctcttttca ccagtgaaga gcaaatgccg aggtccccac
     4621 acgcctgttc aaaagccact tttcacccct aaccacaccg ggtattgagc cgacgggcaa
     4681 gctgaccgag caagccgccc gcttcgtgtt gatcaaccgc cggataacgc agggcacctg
     4741 gagtgaagcc gcccgcggtg cgagcaaatt tcgatcgcca ttacttcctg cgcacagcaa
     4801 ggccgttcag ccttctagtt cttgttggca acggtcgaag ctcctcttcg cggtgctccc
     4861 tgccctcgtt gtcgccatca ggcacttgat tgcgccctca ctgcggttct ttatcatccg
     4921 ggcttgtggc ttggcgttcc ccctgtgtca ggctgtcagc ggttacacgc cccagcaacg
     4981 gagcacagtg agaacatacc acgtggaaag tatatgcgcc ggtgtgtgac gagtagcttc
     5041 ctatccaggt gcgcaggtca tgtcgatgga gagacaccaa ccattcggac gactccgaat
     5101 ttggagccgt tggcaatcgc cgtgtggggc ctcaccagtt ggtgccctgt gcttgccata
     5161 cgggccgacc gccacggccc gccagaccaa ggtaggtggt aggcccagcc gactggtgct
     5221 tcccgtggtg ctctcttttc cggcgaggtg ccatctatgg tgctagactg cttgccgggc
     5281 gttgcatcgg tttgcagcga tttgcacggg aagcgggccg agagttcggc acagcgtcgg
     5341 attgcgtggc cgacacaagt ggcgaagtgg gctagagttc tggccagaaa gcaagacgcc
     5401 gcaagcattt agcacgtgcg gcgtccgagg caagggtgag gcctgaagaa gcgtccccga
     5461 caggactcga acctgtaacc ttcggctccg gaggccgacg ctctatccaa ttgagccacg
     5521 gggacaatac ccacaacacc gtctagcaga ccacaagcga aacgttcatc acctcagcgg
     5581 tctgctagta gtccatttta agccaaccga ggtcttcttg ggaagattgc ccgtcggcat
     5641 atctggacaa ccatttcagg acagcagttg gaacgcgtcg ccaaaggccg attcgagggg
     5701 acttgccgga gtggtgcggg gccgaggtgc ttggggctcc tgcgacagaa tggatggctt
     5761 ggggagttac tcggttctct tttgcggacc tgggaaaagg ggctttcaat ctgatggagc
     5821 tgcgggattt ggcgacttcc ggacaatttg ctaagctgac cattttggtc actcaattcg
     5881 tttggaatca tccaaagagt ctgccctcgg caagtacgca gagatcattt tatgctcaaa
     5941 cggctttcgt cctcccttct cgaaaacttc aaaacgaagc cccagtatcc gaccgcgcct
     6001 atcccgacgg atgggctacc gcccggtgtg ccctacatcg tcggcaacga ggccgccgaa
     6061 cgatttagct tctacggcat gcgggccatc ctgacgattt tcatgacgca atacctttta
     6121 gacgcccacg gccgcttggc ccctatgtcg gagagtgaag ccaaatttta tttccacctg
     6181 ttcaacgccg gggtgtattt tttcccgatt gtgggggcca ttttggcgga catcttctgg
     6241 ggaaaatact ccacaattgt ggggctttct ctggtgtatt gcttgggaca cgtcgccttg
     6301 gcctgggatg aaacgcggtt ggggctattc gtggggctaa cacttattgc cgttggatca
     6361 gggggaatta aatcgtgcgt ctccgcccat gttggggatc aattcgcaag tcgcaatagc
     6421 caccggatgg gtgaagtttt cgcgtggttc tacctggcca tcaaccttgg ggcagccagt
     6481 tccacgctac ttacaccgtg gttgttagtc gtcgccggtc cgggcttggc ctttgctgtg
     6541 cctggcatcc tcatggcatt ggccacggct gtctttttcg ccgggcgggc taagtttgcc
     6601 catattcccc cgagcggtaa gaagttcttt tacgaactga tcagtcccga ggcccgatcc
     6661 accattctgc gattgattcc gcttttctta tgcgtggcga tgttctgggc cctgttcgat
     6721 caatccgggt cggcatgggt ccttcaggcg gagaagatga atcgccgttg gctaggaatc
     6781 cagtgggaat cctcacagat tcaggccgtc aatccgctgc tggtgatgat tatggttccc
     6841 ctttcgacct atgtcattta ccccttagtc aatcgggtat ggcaatttac acttttgagg
     6901 cgtgtttccg tcgggatgta cgtggcagca tgctcctttt gtgttacagc ctggatcgag
     6961 cacacgattg ctgcgggggc aagaccgagc attgggtggc aggtcgcggc ctacgtagtg
     7021 atcacagcgg cggaaatcct catctccgta acctgcctgg agttcgccta cactcaggcc
     7081 ccgcgtacca tgaagtcagt gatcatgggt ttatacctgc tgtccgtatc gcttgggaac
     7141 tcattcacgg ccgtggtcaa taagttcatt gaactgaccg gtggcgaccg acccggcggt
     7201 tgggcgcagg gacccggtta ttattggttt tttgtgcttg cgatggcgtt gacaggcgtc
     7261 gtatttacga ttgtttcccg gttttaccga gagaaaacct accttcaggc atctgaaaca
     7321 tagacgccat agcctttgcc acaccgtctt tagccgcggg cttttctccg aagttcgatt
     7381 cccgtttttg cgttccgggg ggcttggcaa aagtggccgg tagatgcttt aatacgggaa
     7441 cccagtgctt aaaggcgctt cgcgatcgtg tgacgcttct gtgctttccc acctgtgatc
     7501 gggataaccc tttccgcagc gagcgccgag cgcgacgcca ggtccccatt gagatgcctg
     7561 gccgaagcat gtagaatcga ggtttttcac cttttgcgga aatagttggc cagtcggtgt
     7621 cgccgggcgt ggcgaggata gcagatttat gtcggcaacc attaacgaaa ttattcgaat
     7681 tcggatggaa tcctacgatc acgcggtctt agaccagagt gcccgcgaga tcgtcgatac
     7741 ggttaagcgg acccattcca aggtccacgg cccaatccct ttgcccactc gaattgagcg
     7801 atataccgtc ctctcaagtc cccatgtgga caagaaggcg aggcaacagt tcgaaatccg
     7861 cacacacaag cggttgatcg atatccttga ggttacgcct gcaaccattg aagcgctaaa
     7921 caaattgaca ctcccggccg gtgtagacat caagatcaag gcaaccagcc gggggcgaag
     7981 tcgctaaaac ctaaaacctt gtgcagtgtt gggttgtttt caacgctgaa accaaaggcc
     8041 agaaccagac gataaacatc catcgcgtgt atggactgga agccccagag gtaatctggg
     8101 accaatccca cgatcctcga gggcaatgta gataaggtag ataaacaatg gcgatcggat
     8161 tacttggccg caaggtcggc atgacgcaaa tttttgatga tgcagggaac gtgatccccg
     8221 taaccgtgat ccaagcgggt ccctgccaca ttttgcagat cagaaccccg caacgcgacg
     8281 ggtacgaagc catccagcta ggctaccttg acaagccccg ccatcgagca aatcgggcag
     8341 agcgcgggca tgtggccagg ttagaaagca aacgtcaaaa gctcctcgcc gctgccggca
     8401 tccaagctcc tcccaaagcc aactgcgagc cgaagcgttt catcaaagag ttccggactt
     8461 ccatcgaagg tttctccgta gggcaggttc tgacagtcga tcttttcaag gatgttgccg
     8521 cagtggacgt gaccggcacg accaagggtc gcggctttac aggtgtgatg aaacggcacg
     8581 gcttttccgg ccagcgagca agccacggtg ttaagaacgt ccatcgccac cctggttgta
     8641 ccggctgtaa cacttctccc ggtcgcgtcg tccctggtcg gcgaatggca ggccactacg
     8701 gacacgaaaa atgcacaatt cgcaatctga aacttgtgcg gattgattca gagcgtcacc
     8761 tcctcctggt caagggtgca gttcccggac cggtaggcgg attaatcatt attcggccga
     8821 caaacaaggt ggggtaagcg aggggaagca acattctgta ggaccaaaaa cgagagaaat
     8881 gacgttaggt cccacaaaga agcaggtgta tcatggtcag tatcccagta tataaccgcg
     8941 aaggccagca ggttgacacc tatgagatcg atcctgccga gctggcgccg cgcatcaaca
     9001 aacagctatt gcatgacgca gtcgtcatgt accgggccaa cctccggctg gggacggcaa
     9061 aggcaaagag ccgttccgag gtggccggtt cgacgaggaa gctttatcgt caaaagggaa
     9121 ctggtcgggc gcgagcgggt atgcggcgga cacccattcg gcgtggtggt gggcgtgccc
     9181 atcccaagcg acctcaggat tggtcttacc gcctcccgaa aaaggccctt cggcttgcta
     9241 cccgaatggc tttggcgtcc cgcatcgctg ataacgaagt gatccttatc gacgaattga
     9301 agttcgaggc tccgaagacc cgggaagcag cggggatcct caataaactt ggattggccg
     9361 gaatgcggct gctggtgacg ctcgacaatt atgatccgat tgcttacaag agccttcgca
     9421 atatcgacaa agttgagacg ttgccggtgt cagacatcaa cgcgtatgag gtccttcggc
     9481 caaagcgcat cctaatgacc cgtgcagcgc tcgacatgtt tcgtgccaag gtcgtggccg
     9541 gtaccgcttg agtgagaagg agatcgagcc atgttacgca atacccctca gcctgctgtc
     9601 tccggtttgg agccacatca agtgatcatc cggccgttga tcacggaaaa gagccatcat
     9661 aaggcggagc gctacaacca atacgcgttt gaggtctcgc ccttggcaac aaaggcggat
     9721 atcaagaagg cagtagaaga attgttcaat gtgaaggtcc ttcgggttaa cattcagaat
     9781 cgccgcggca aagaacgacg ttatcgctgg cgcaaggcct acacgaaggc gtggaagaag
     9841 gccattgtta aactggatcc agagtaccgg atcaatctct actaagcaga tcacactttg
     9901 aggatacgag gccagtatca tggggattcg tgcctacaag ccgacatcac cggggagacg
     9961 gggagccacc gtcagcgatt tcgcggaact gacgcctggc gctgagccgg aaaagagtct
    10021 ccttgttccc ctgaaaaaga aaggtggccg aaataaccaa ggcatcatca cctgccgaca
    10081 tcgcggtggt ggccacaaga ggctttatcg gatcatcgac tttaaacgga acaaggatgg
    10141 gattccggcg cgggtggatt ccatccagta cgatcccaat cgcagtgcgc ggatcgcctt
    10201 gctgaagtac gccgacggcg aaaagcggta catcattgcc ccggatgggc tcaagccggg
    10261 cgataccgtg atgagtggtc ccggcgcgcc cccgaacata ggaaacgccc tgccgctatc
    10321 ggagattccc ttgggtatgc aggtgcacaa cattgaactg atacccggga aaggtggcgt
    10381 catgtgtcgc tccgcgggat gcgcagccac gctggtggcc cgtgacggag attgggccca
    10441 attgacactg cccagcggcg agattcgtcg tgtctcggct cgttgccgag cgacgattgg
    10501 tgtggtcagc aacgccgacc atgaagggat tgtcttgggc aaggctggcc gaaagcggtg
    10561 gctcggtcgg cgaccacacg tccgtggtac tgccatgaac ccgatcgatc acccccacgg
    10621 tggtggtgaa ggacgaacca agggcggacg gcatcccgtc agccccacag gcaagcccgc
    10681 taagggcgga tatacccgga agcgcggtaa accatcgaat aaggcgattg tccggcggcg
    10741 tcggtccgtg cgatatggtc agcttccgat acggtgagaa aaacagtcaa tagccaagtg
    10801 attttggatt tcaggcggaa aaacgactat gggacgttca ctgaagaaag gtccgtttgt
    10861 cgatcccaac gtcttcgcca aggtggctaa gctcaacgag gcgggaaaga aggaaccgat
    10921 taagacgtgg gcaagggcat gtaccatcgt gcccgagttc gtcgggcaca ccttcctggt
    10981 gcacaacggc aagacacacg tcaaggtatt tgtcacagaa gatatggtcg gacaccgcct
    11041 gggcgagttc gcgccgacgc ggattttccg cggccacggc ggacgtggga agaagtagtt
    11101 ttggaggtat aggccaagag gcgatttggc aagctccgaa ggttccagta gatagcagcc
    11161 tcggagcaaa aaggacaagg tcggaatcat gtccatttcc aatatgtcgc gaatgtatcg
    11221 agccttacat cggtacgccc gaattagccc gcgcaaggcg aggctggtcg ctgatgttgt
    11281 gcgtggcaaa caggtcgatg aggcccttgc tatattgaag accatgcccc atcgcgctgc
    11341 gcgcatgatt gaaaaggtgg tgagcagcgc cttggccaat tcggctgacc tgcaggatcc
    11401 gttcccgcga gagttacatg tcatccgcat ttgggtggat gagggtccac gattgaagcg
    11461 acttcgtccg ctgtcccgcg gaatggccac gatcattcaa cggcggatgt gccatatcgg
    11521 cgtgattctc ggacctcgtg aataccttaa gtaatttggg tgggattatt gaggatacct
    11581 ggttatggga caaaaggtaa accccatcgg ttttcgcact ggtatcacgc tcgactggaa
    11641 gagccggtgg atcgcgccga agcgcgagtt cgctgatctc ttgctcgaag actataagat
    11701 ccgtcgttac attttggagc ggaaggagcc atccgcggaa aaagggggac gcggcgaacg
    11761 acgtgcctat cccatgatct cgaaaatcga gatcgagcgg acgcgcgatg aagtgaaggt
    11821 gatcatattc acggcccgtc ctggtgtgct aatcggatca aaaggcggga agatcgacca
    11881 gctccagcag gagctccagg agctgaccgg gcggcggatc aatttgaaga tcgaagagat
    11941 cacacgtccc gagctggtgg cccaactggt ggccgaaagc atcgcggaag aactggagcg
    12001 ccgcgttagc tttcgacgtg ccatgaagaa ggccatggaa acagccatgg cacaaggtgc
    12061 ccgaggaatt aaggtccagc tctccgggcg gcttggcggg gccgaaatgt cgcggcgtga
    12121 gaaacaaatt atgggatccg tcccactctc cacgcttcgg gcaaatatcg attacggctt
    12181 tgccgaagcg acaattgctc agggacagat tggcgtccag gtgtggatca atttgggaat
    12241 gtttagcgag gttgatagcc atggcgttga tgccaaaacg ggtcaaacat cgaaaaagtc
    12301 aaagagggcg tataaggggt aacgcgacac ggggtaaccg cgtcgtgttc ggcgaatacg
    12361 gcctccaggc tgttcagggt ggatggatcc ctgctgccac gattgaagcc ggacgtattg
    12421 ccgcccagca atacctccgc tcggaaggtc ggctgtacat acgaatcttc ccccacaagc
    12481 cgattacctc aatccccttg gaaacacgca tgggtaaggg aaagggggaa cccgaatact
    12541 gggcagccgt ggttaagccc gggactatcc tatttgaact cggcggtatt tccgaggagg
    12601 ccgctcggtt gtgtttttcg cggttggccc acaagatgcc ggtgaaggtc cgatttgtcc
    12661 ggcgccagac catgtaatgg cttggccagt gggatgaaac caacaggaga tagggaccgt
    12721 ttaagtcggg tcaagggtga aaggtaacag acatgaaagc ccgcgaactt cgggagatga
    12781 gcaccgaaca actcgaagcc ctgctccgcg agaccaccga ggccttgttt cgtttgcgga
    12841 ttcaaaagca aacggagcgc ttggcgtctc cgatggaacc gcggatgaag cggcgcttga
    12901 ttgcccggat caagaccatt ttgaacgagc gagcccgaca agaggcatcc gcggcaaagt
    12961 aatctggaca acaccggagg cgctgaagaa tcgtccggca gggaaaacat ttgcgaggtg
    13021 aaaaacaata tgccaaggcg acgtgaaatc ggcatcgtaa caaaggcgaa tacaccccag
    13081 ttgcgtcggg tggaagtcat ccgacttgtt cagcatcctc gctataaaaa gtacgtgcga
    13141 catcgtacga tttgctacgt tcatgacgag aacaacgagt cgcaagtggg tgaccgagtc
    13201 gagattatgg agtcgagacc cctatcgcgg ctcaaacgct ggcggttggt acgtgtcgtg
    13261 gagcgcgggc gtgccgaatt gctccaaaag cgacgtgtgg ttgaggagga attgctggag
    13321 gccagtagga ccgggaccga agccagggag gcttcgccgg cttcacagga aacacaacag
    13381 tcggatcagc aggggtaatg ttgccgactt gttcgcagca ttcgatggtc ggcaggaatt
    13441 gcggaaacaa gggtgatcgc catgatccag atgcaaacca ggctcgatgt cgccgacaac
    13501 acaggcgtca aggagctgat gtgtattaag gtcctcggcg ggagccgccg acgttatgca
    13561 accctcgggg atattgttgt ctgcagtgtg aagggcgtga tccccggtag cacctttcaa
    13621 aaaggggctg tcgtgcgggc cgtgatcgtt aggacgaaga agccgaaacg gcggcccgat
    13681 gggagttacg tccgttttga cagcaatgcc gccgtgatcg tcgataacga cggcaatcct
    13741 cggggaacac gtatttttgg tgcggttgct cgggaattga gagaacggaa tttcatgaaa
    13801 attgttagcc tcgcaagcga ggtggtctga tgtggatacg aatcgatgac actgtggaag
    13861 tgattagtgg ggacgaccgg ggtgttcgtg gccgcgtctt gcgggtggat cggaaacagg
    13921 gccgcgtcgt tgtggaaggc gtgaacatcg tcctgaaaca cgtgcggcga agccggcagg
    13981 cgcgccaggg cggccggctg tcgaaggagc ggcctatcga catttccaac gtcatgttgg
    14041 tctgcccttc gtgcaagcag cggacccgtg ttggggcacg cttcctcgcc gacggctcca
    14101 aggttcgtta ttgcaagaaa tgtgatgcga acatcggaca gatttctagg ccgaagtcgc
    14161 ggtcacaaac tgcgaatgcc tgatatcggg ggataagctg aatatggaac cgcgtctttt
    14221 ggaaaaatac cgaaaagagg tcatcccgat cctgatggaa aagacgggcc ggaccaaccg
    14281 gttgtctctg ccccgcctgg aaaagattgt gatcaatatg ggggtcggtg aggccaagaa
    14341 agaaaagaag tacctcgatg aggccgtgga tgcccttacc aatatcgcgg gccaaaaggc
    14401 cgtgatcacg cgggcgagga aatccgtggc cgggttccag gtacgccagg ggatggcgat
    14461 cggttgtaaa gtcaccttgc gtggtcggcg gatgtatgag ttcctcgacc gtttgatctc
    14521 catcgctctc ccccgtgtcc gcgactttcg cggattaaac cctcgggctt tcgacggaca
    14581 cggcaattat gctctgggcc tgaccgaaca aatggtcttc cctgaactga accccgacaa
    14641 gtattcgcgg aaccagggca tggatatctg catcgtcacc accgcccgga acgatcgcga
    14701 ggccctggag ttgctcaagg cattggggat gcccttccgt gcggaatgat gagacaagag
    14761 gaaaacctgg gtttttagga tagcggccaa tggcgagtaa atccaagatt gcaaagtttc
    14821 gtaaaaagcc aaagttctcg acgcgatttc ggaaccgatg ccgtatttgt ggtcggccac
    14881 gcgcggttta ccgaaagttt ggtatctgcc ggatttgctt ccgcaaattg gccgacaacg
    14941 ggatgattcc aggcgtagtc aaggcgagtt ggtagagggt aatacacgac catgatgacc
    15001 gacccgatcg ccgatgtgct gactcggatt cgtaatgcgg tgcgtgttga acgaccgcac
    15061 gtggatgttc ccacgtcgaa cctgaaacga ggtctggccg atgtgctgaa gcgcgaaggc
    15121 tatgtttggg attgggaaga gattccaagt cagcctcgag ctattttgcg aatttacttg
    15181 aaatatggac ctaacggcga acaggtcatc cgccatatcc gacggattag caagcccggg
    15241 cgacgggttt acagccgtgc ggcagacctt aagccggtcc tgaacggctt tggaattagg
    15301 attttgagca ccagccgcgg tattatgagc gaccgcgagg cgcgacagaa aaaagtcggc
    15361 ggcgaaatct tgtgcgaagt ttggtaattt ttggtcaatg ccttaggact ccatcatagg
    15421 aaatctgtta gccatgtcgc gtattggaaa acgacccgtt ccgattccac aaggcgtgac
    15481 cgtcacgatc gagggtcggg taattcgtgt caaaggcccc aagggtgagt taacgttcga
    15541 gcatcgcccg gaggtgtccg tgtcagttga tacggcacgt tcggccgtcg ttgtcgaacg
    15601 tgttcaagag acccggctgg cccgggccct gcacggacta acacgggccc tcgttaacaa
    15661 tatggtgatt ggagtcaccc agggctacga aaagaggttg gagatttccg gcgtgggtta
    15721 tctggctgcg gtgcagaaag gggtattgca actccgcgtg ggttatgcca acgagcttca
    15781 agttcccatt cctgagggac ttgaggtgac ctgtccggat cagcaacaca tcgtgattcg
    15841 cggtgtggac aaacaaaagg ttggcaattt cgccgcggcg gtcagggcgc ttcgtaagcc
    15901 cgatccgtac aagggtaaag gcatccgtta ccagggcgag cacatccgta tgaaggaagg
    15961 aaaagtgtcc gccaagtagt ccccgggaaa cgatgacgtg tcgcatgaac gcgtgaggat
    16021 tgtttcggga gtcgcccagc gggtgcggta gaacaacagg aaataacgag cgagagtgca
    16081 agtcaaaaag agaggccagt cgtgagtcag ctcaagcgat tgcaaagacg tcgattgcgg
    16141 cgagtctttc gagtgcggaa caagctccgc agggtctcga accgaccgcg actcagcgtg
    16201 ttccgcagca acaagcatat ttatgcccaa gtcatcgacg acagccaagg acggacgctg
    16261 gccgcggcaa gtacggtgga gccggcgatc cgcgagatgg tcggtggcta cggtggcaat
    16321 gtgaaggccg ctgccgtggt gggaaaagtg atagcggaac gtgcccttgc ggcggggatc
    16381 acgaaggtcg ttttcgaccg cgggccgtat aagtatcatg gtcgggtggc cgctctagcc
    16441 gatgccgcac gagacgccgg cctggatatt ggcccgaaga aagcaccgga agcggagccc
    16501 gccgaggaaa aaccgaagaa gaagggcgag aaggcaacct ctggaaaagg cgagaaagct
    16561 ggaaagaagg gttgatcaac aacgagaaca ttccctgtaa aggttgaaac atggcgtcag
    16621 atttggaacc cattcgtggt caattgatcg agaaggtcct cagtgtccgt cggtgcgccg
    16681 ccgtcgtgaa gggaggccgc cggttcagct ttacggccct cgtcgttgtt ggtgatggga
    16741 tgggcaaggt cggctggggc tatgggaaag ccaacgaagt gcctcctgcc gtggcgaagg
    16801 ccatcaagga tggcatgcgg agaatgaaga ccgtgacgat tgttgatggc acgattccgc
    16861 accaggtcaa aggttcgttc ggggccgctg aggtcgtcct ggtcccggct ggacccggaa
    16921 ccggtgtgat tgcgggcgcc gcggttcggg cggtgtgcga agccgccggg atcaagaaca
    16981 tcctgacgaa aagttacgga aacaccaacc ctattaatct cgtgaaagca acaatgaacg
    17041 ccctggagca actccgttcc aaggctgaag tggaacgctt gagagggaca tcgttgtcat
    17101 gaatatcgac caggtcaatc gaggagtcgt cacccacaag aaacgcaagc gagttggccg
    17161 tggcccgggt tccgggcacg ggaagacgtg cgggcgggga cataagggtg cccgctcgcg
    17221 gagtggctgg cgagaaaagg caggcttcga aggcggtcag atgccgctca ttcgccgggt
    17281 tccgaagcgc ggcttcaaca atgcccgttt tgccctgcga attgcggaga tcaatctcga
    17341 ggtgctggag aaggccttcg cagatggcga tgaggttacg cccgaaattc tcgaacagcg
    17401 cggccttgtc aaacgggttt acgacgagtt gaaaatcctg ggtaatggcg aattgaccaa
    17461 gaaactctcc gtttcggcgc accgttttag ccgttccgcg aaggaaaaga tcgaaaaggc
    17521 aggcggcaag gttatcgtct tgcccggcaa gacgcccgta gaagaaaaga agaaggcggt
    17581 caaaggagca taagcgtaaa cgcgtcacga gtgacctcgg cgctgtgatg gacggaccgg
    17641 cctatgtggg aaaagattcg tgttatcttt acgattcggg aactgagaca gaaaatcctg
    17701 ctcaccctac tgctcttggc gatctatcgg atcggtgagc agattcctgt tcccggtatc
    17761 aacaccgaga aaatgactgc catcttccgc agtcaggcag cgggcgggtt gcgcgatctg
    17821 ctggaccaag taaccgtctt cgctgccagc cggttggacc aagccaccat ctttggactt
    17881 ggtattatgc cgtacatctc ggcgtcgatc atcttccagc ttcttgcgag cgtctatccc
    17941 cctctggaga agctcaagaa ggagggaccg gccggttaca aaaaaatcaa cgaatatacc
    18001 cggtacgcca cggtggggat atgcattatc caaagctgga tgtatgtcgc ctggctcgct
    18061 gcgcagggat tcgtcaggcc cgaatatacc tatggtgaag gccagggagt gtatttctct
    18121 tggttcctga ccgccgtgat caccatgacg gcggggacaa cgttcctcat gtggctgggc
    18181 gagcagatcg acgaatatgg aattggcaac gggatcagct tgctcattat ggccggtatc
    18241 ctggcaagaa tgattccggc cctttaccaa gagttgcgcc cggtcttcga tgagggactt
    18301 gaactaagcg gcactgcggg aaagatcggc tttgacacgt tgatgatttt gctggtgctc
    18361 tttgtgtccg ttgtggccgg tgtggtctat attacgaggg gccaacggcg catccttacc
    18421 cagagcgcca agcacgtccg gggacgccga gtttacggtg gcgcccgcga ttacctgccc
    18481 cttttggtca atcaggcggg ggtgatgccg atcattttcg ccagcagtct cctcctattt
    18541 ccgttgacat tcttccaaac cctggcccac tggtatccca attcaagctg gttggcggcg
    18601 gcggctaacg tgttccaccg gggggatggg ttctggtaca acgcgatgta cattggtttg
    18661 atctatttct tctgctattt ctggaccgct atcaccttca acccgaatga gattgccgaa
    18721 cagctcaagg acaacggaag catcattatg ggatatcgtc cgggacgaag gacggcggag
    18781 taccttgaga aggtcatggt gagaatcacc tatgtggggg caggcttttt ggcggcggtt
    18841 gccatcatcc ccaccattgt ggctgattgg ttgggagtca attatcagat cgcccagttt
    18901 tatggtggaa cgagtttgct catcgccgtg agtgtggcta tggatttggt acaaaagatc
    18961 gatagccaca tcgtcatgcg gaattacaag ggattgctcg agtagtattt tcgctgggca
    19021 gtcaggtaag cgtgagagtc tccttcgttg taccattggc aagtcggttt taggattatc
    19081 cagcgatgcg attggtattc attggtccac cgggcgcggg aaaaggcacg caggcggaaa
    19141 ggatcgtctc gcggtacatg atcgcccacc tttccactgg cgacatgcta cgggcggcga
    19201 gaaatgcaaa aactccattg gggattcaag ccgacgctta tatgtcgcgg ggcgagctgg
    19261 tccccgatga tttgattatt gatttaattc gagagcgatt ggccgcggac gactgccagc
    19321 gtggttacct gctcgacggc tttccaagga ccatcgccca agccgaagct ttagacaaaa
    19381 tgctcgggga gaaaaagacg ccactggacg tcgttttgga attgcgggtt cccgaagaag
    19441 aattgttccg gcgcctggcc ggtcgtgggc gagccgatga caaaccggaa gtcatccgac
    19501 agcggcttgt cgcttaccga gagcagacgg cccccttgtt ggactactac caagagaagg
    19561 gattgttgaa gaccgtcgat ggtctcggga ccgtggacga ggtcttcgag cggatccgga
    19621 gtatcctcga cgagtttgcg ggagcttgag gggtgagttg ggccaggaga gtcgcaccag
    19681 tgggactggg agtgagccag gtgtgataac gttgcggtca cgatcagaaa ttctcagcat
    19741 gcgaagggcg ggccttgttg tatggaaagc cctcgagtta gtcaagtcca tggtgcgacc
    19801 gggcctgatg acaaaggaga tcgaccaggc cgttgaggat tttttcaaac ggtctggtgc
    19861 ccagccgctg tttaaaggtg ttcccggacg tgtccccttc cctgccgtga cctgcatctc
    19921 cgtgaatgag gaggtcgtcc atggcatccc gggcgaccgc cagatcaagg agggtgatat
    19981 cgtcagcgtg gacacgggtt gccgtgtcga tggctggtgt ggcgatgcgg ccgtgacgat
    20041 ccctgtgggc cgtccggctc cggaagtgct caagctggtg gaggtcactc ggcagaccct
    20101 tgaccttgcc atcgagctga tgggacgaag tcgattgtgg agtgaagtgg ccgcggccat
    20161 gcagcggtac gtcgaaaagc atggattcag tgtagtcgag gcgtttgtag gccacggaat
    20221 cggccgatcc atgcacgagg aaccccaggt tcccaatttt gtaaacgcgt tgtttttgaa
    20281 aaagggcgat tttgcccttc agcccggcct ggtgattgcc atcgaaccga tggtcaattt
    20341 gggaactaaa aaggtacggc tctgccggga tcattggacc caggtaacgg ctgacggaaa
    20401 accgagtgct cattttgagc acacggtggc catgaccgag tttggcccat atgttttgac
    20461 cgggccatta ggccccatgg atgagaagct cggttttgat cctgatcgac atcattttgc
    20521 gtgatttcgg gagcggtcgc ggaggaaata ggaaataaag ggaaaagact aagcaaaccg
    20581 gcggcctgcg ggggccttga gtcgccaaag caaggcgctc tatactaaag gtttcgacag
    20641 cactgccaac cacgagataa cggggattca gaatatgaaa gtgcgagcga gtgtcaagcg
    20701 tttatgcagt aattgtaaag ttgtccgcag gcagggccgg atttacgtca tttgctccaa
    20761 tccgcgtcac aagcagcggc aagggtgatt atttggaaaa atagaagaaa cggttgactg
    20821 attgatcacc aggagcggca aatatgccac ggttgcttgg ggttgatatt cctggaaata
    20881 agcccacggt tatcgcgttg cggtacatat acggagttgg gccagccatc gccaaagagt
    20941 tatgctacaa ggcggggatt gatcctcaca ttcgtgcgaa tcaactccga gaggatgaac
    21001 tggcgcgcct ggccacgttg ctggacaaag actacaccgt tgagggccag ttgcggcgtc
    21061 agatcgccca aaatatcaat cggctcaaag acatcaactg ctatcgtggg attcggcacc
    21121 gtcgtggcct tcctgttcgg gggcagcgca cacggacaaa cgcccgaaca cgaaaaggcc
    21181 cgcgaaagac ggtcgctggc aagaagggtg tgaaagacct gcggtaagct ggatttcgtc
    21241 gcgatattta tcagggttat aagggtcgga aaagactgcg aggttttgga aagtggctaa
    21301 gatcggtcca aagcgaaaaa aaatccgaag gcacgtcacc caggccatcg tccacatcaa
    21361 gacaaccttc aacaacacga tggtcactgt taccgacctg aagggtgaga cgctgtgctg
    21421 ggcgagcgcg ggaacgtgtg gcttcaaggg aagccgcaaa agtaccccgt acgccgggca
    21481 aatggctgct cagcaggcgg ccgagaaggc catcaaaatg ggggtcaaag aggtagaagt
    21541 caaggtgaaa ggaccaggcg ctggtcgaga aagcgccatt acggccctcc agacggcggg
    21601 tcttgttgtc aagtcgattg aggatgtcac cccgctgccg cataacgggt gccgtcccaa
    21661 gaagcggcgt cgggtgtgat gggaaacacg ctgaaaaatg atgggttgag gttgtctacc
    21721 aactgagtca gctttcatcg tcaagacaag gactgcgtta aaacatgggt cgatatactg
    21781 gaccggtttg ccgactctgt cgccgcgagg gaatgaagtt gttcctcaag ggcactcgct
    21841 gcgatacgga caagtgtgcc ttcgaaaggc ataaggccac acccgggatg cagggtgtcc
    21901 tgcgagggaa acccaccgat tacgccctcc acctgcgaga aaagcaaaag ctcaagaggt
    21961 tctacggcat ctatgagcgg caattccggc gttatttcga gatggcaact cggtcgccga
    22021 ctaacaccgg cgaggtgctc atgtcgctgc tggagcggcg tctggacaac atcgtttacc
    22081 gtttgggatt cgctttgtca cgggcacagg cccggcaatt ggtggcgcac ggccacatct
    22141 tagtcaacgg ccgcaaggtc accattccca gttacttggt gagccccggc gatgtcgtta
    22201 gcgtcaagaa tcggccgcgg agtttggatg cggttcagag gaatctggcc gagagacaac
    22261 ccatggtgcc cgatttcctg aacttgatta ctggcccggt tccggagggg catgtgcttc
    22321 ggaacccaac gttggaggat gtgtcactcc cgattcaacc tcaacttgtg atcgagttct
    22381 gctcgcggta agaaagacaa agggtaaccc aaggtttaag tctaccggag gccttccatg
    22441 cgggtacagt ggcgcgggct cgagttgccg agccaggtgg tttgcgaccg atccactctc
    22501 accgaccgat acggaaagtt tattgccgaa ccatttgaac gcggcttcgg cgtaaccatc
    22561 ggtaatagct tgcgtcgcat cttgctttcc agtttggaag gcagcgccgt gaccaaggtg
    22621 aagatccagg gggccttgca cgaaatcacg tcccttcccg gggtcgtgga ggatgtgacc
    22681 gatatcatcc tgaacgtcaa ggccctggtg gtgcgaaacc acagttccca gccaaggaca
    22741 ctgcgtatta accggtgcac caagggggtg gtcactgccg ccgatatcga acctgacgac
    22801 caggtaacaa tcatcaataa ggaccacgtc atcgcaaccc tgaccgatga cgtacctttc
    22861 gtgatggaaa tggtggtaga gaatggtcgt gggtatgtac cggccagtga acacacaccc
    22921 cagatttccg agatcggtgt gattccggtc gatgccatgt tcagccccgt ggtcaaagtt
    22981 cggtacgagg tcgaagaaac ccgtgtcgga cagaagacca attacgacaa attgacgatg
    23041 gaaatttgga cggatgggtc aatcaccccc gagatggccc tcgtagaggc cgccaagatc
    23101 ctccgtaagc acctcaatcc gtttgtccag tacaccgagc ttggtcccaa ggtcttcgcc
    23161 gacagtcgat ccgtcctgag ccaggattca acgctcgaca gcaaactgag tgtctccctg
    23221 gccgatttga agttttccac aagaacgctg acgagcctgg aaagtgccgg tatccatacc
    23281 gtgcgggacc tggtggcgat gacagaacag caactcctgg atttgcggaa tttcggggag
    23341 acgtcgctgg ccgaagttaa ggatcgcctg gccacattgg ggctgcgatt ggggatgcga
    23401 acatcgccat ctccgggtgg cgttgtttaa tgtgtcaaag aatcattgta gtaggaaata
    23461 gagagattag ttttgcggga taaccgtccg gttaggatgt aaccatgcgg catcgaaaaa
    23521 aaggtcgata tttaggccga agtccaagtc accgtcgtgc cctgctgcga aatttggctt
    23581 cctcattatt cctgacggaa ggagatttcc tctttgaaca tgaggaacct tatgtaaaag
    23641 gtaggattat caccaccttg gccaaagcca aggaggtcag gcccctggtt gagcggtgca
    23701 tcacgattgc caagcgagtc ctcccgcttt tggaagaggc caaacgcatg gagccgactg
    23761 cccctcgtaa ctccgaggct tggcggcggt ggcgacaaag cgacgcctgg tatgaatgga
    23821 accgcaagat ggcaccggtc gtggctgcgc gacgccggat cttgcggatg ttgggaaaca
    23881 acaaggaagc cgttcgggtg ctgttcgaaa agatcgcgcc acgcttcgtt gaccgtcccg
    23941 gtggttatac ccgaatcgtt cgcctagcca agcggcgggt cggggatgca ggtgtgaagg
    24001 cgttcattga gttcgtgggc caaaacgatc gcgtcaagca acgaaagact atccgtccag
    24061 aattcagcgt cgaaacaccc aaaccgtcgg aacccgcccc tgtgaaagag gccacgatcg
    24121 ataccccaga agctgcgaac gtttcccaag agaatccatc aaactgaacg ctgcgaactc
    24181 tttctgccct ccccttccca ccccgccggt ttttcgaaac cggcgggttt tttgttcgac
    24241 cgggccacat taatcacggg tttctctagc acggaagatt ggaggcacat ccattgggcg
    24301 aggggtaaac ccatttttta ttgcacgcca tccccctcac ccaccagacc acccaactgg
    24361 ggagagtcgc attgattttt ttgggaattc ttggtactgt cctgtccgcg ctccatggac
    24421 tagcgctgac aggattcttt ccatttggca cagtactgtg gacgagcccc cttctgggga
    24481 gactcgaaaa gtcttcaaat gcggttaccg actggtgatt accaaaggat tgattggaag
    24541 acctcgcacg ggatgacaac gagagttgcc gcccattttg aacggatgtg tgccctcctg
    24601 gtgattcttg ggatggccag tggttgcacg catctccgca tgcccgcgag tagtcctttg
    24661 gtcccagccc agatgtccag cgacagcgtc gctctcgaca tcatctttgc ccacgtccct
    24721 ccctccgatg acgtgctaaa ccaaatctgg accgagctcg acgaacaatt cctgccccag
    24781 gaaactcgga agggcctcta ccaaaacggt ttccgcatcg ggagatgtgc aggaccggtg
    24841 ccgcaatcgc tggccgaagt aatgaagctc actgacagcg gaccagccta cagcgaggta
    24901 aacacggccg atgccatgac gctaacggac gacgataccc ccacccggcg tcatctccaa
    24961 ttgcggacgg gatgccgcac cgagattctc gcctccgccg tttacgagga actccctttt
    25021 ctccgccgcg aggatggccg gctcgtggga cgcactctcc caaaggccca aggtgtattc
    25081 gccctgacca cgcgtccggg gacatatggt ggtacccgaa tccgacttgt ccccgaagtc
    25141 cattttggcg agcctaggat tcagcccatc gccaagcagg gtatgatccg atgggaatcg
    25201 caacgagacc gtattgtttt ggatgatctg atcgtcgaga cggaacttca ccccggggat
    25261 ttgcttgttt tgacgtgcct tcccaactgc ccaggcagtc tgggaaaccg cttttttacg
    25321 gtgagctccc aagggaaaat gcagcaaaag attattatta tccgtttgtc gcaaacgcag
    25381 gcctcgtcac tcttcacagc gaacgaatag gattcgtctt ccccttcaag ggcgcaactc
    25441 gggtagaacg cgacttgctg tcttacggat cggcgagaaa agagccgcga cccggacgct
    25501 gccgaatcgc ggcacgttct ttcttagcac cgtttaccgg acaacgagtc agcgagtttt
    25561 tgcttcaacc accgcgaacg aaagcggcaa cttccttgag ctggcccgcc gaccctagtt
    25621 tcttattctt gtggatgatg tactccgcat aggcggccat gaagaccttg cccggtgtcc
    25681 tcgggtcaaa atcttcaggc ttatcgcgga atgtctcgcg atagacggca caccatacga
    25741 gcctgccatc agtatcgatg ttaattttgc acacgcccag cgcggccgcc ttcgcgtagt
    25801 cgtcttcatt cacgccgctc gtctgcttta gtgctccgcc ggcggcattg atgcgatcca
    25861 cccattcctt gggaacacta ctggcaccgt gcatgacaag gggataaccc tccgggaggg
    25921 ccttttgaat ct
//
LOCUS       DAOSUT010000049        25553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1266_length_25553_cov_10.113774, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000049 DAOSUT010000000
VERSION     DAOSUT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 25553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25553
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_25553_cov_10.113774"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1185)
                     /gene="thpR"
                     /locus_tag="PLD05_06295"
     CDS             complement(610..1185)
                     /gene="thpR"
                     /locus_tag="PLD05_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOP77080.1"
                     /translation="MKNKVRTFVAVEIDDAVRNRAAKFIKQLRQSGADVAWVAPENMH
                     LTIKFLGDVEYKDVYHVCRAIEQSLADVEPFSIEIKGTGAFPSLSRPHVIWLGVELGA
                     SEMAALSERVEDALSGLGFRREARRFHAHLTLGRVRKSGRAIEHLVAMLREHEDTDFG
                     PSVVDQVVIFSSELHPTGPIYEPMGHVELKG"
     assembly_gap    1369..1468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1594..2937)
                     /gene="glmM"
                     /locus_tag="PLD05_06300"
     CDS             complement(1594..2937)
                     /gene="glmM"
                     /locus_tag="PLD05_06300"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOP77081.1"
                     /translation="MAEPIISVSGLRGIIGESLTPEVAMRFAAAWAATAPAGAIVLAR
                     DGRASGTMIHAAVLAGLLGTGRTVLDANIAATPTVGVLVQHYRAAGGIQITASHNPPE
                     YNGLKLFSSEGRVIPSTLGQEVIKRYREGRLEWVRAARVGTREICPDTVTPHLNAILK
                     TVDVNRIRACHFRVLLDSNHGAGSVLGLPLLEALGCEVVPRGEIPNGAFEHPPEPLAE
                     NLLSICDAVRQCQADIGFCQDPDGDRLAIIDASGRYLGEEYTLSLCLDHRLKTRKGPV
                     VTNCASSRMNQDIAQKYGVPFFRSAVGEANVVDAMIAHDAVFGGEGNGGPIDPRVVLV
                     RDSFVGMACVLDAMASTGCTVAELAANLPRYAIVKTKVEFPTEKLEAAYHAVMRQFRD
                     ARADRLDGLRLDWPDRWVLLRPSNTEPIVRIIAEAPLEEEAKQLCGTVANVLKGL"
     gene            3660..>4840
                     /locus_tag="PLD05_06305"
     CDS             3660..>4840
                     /locus_tag="PLD05_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOP77082.1"
                     /translation="MVVEYDVIRDSLARLEEAEQNNSVSPSAARNIRRWLTEAEFTEY
                     APQIVEMIQAGRWQELNDVFWQIIPFGTGGRRGRMFPVGTNAINDRTIGESAQGVADY
                     VRSVFGEKALSCVIAYDTRHRSRHFAELCAEVMVASGFTVFFLDGFRSTPELSFAVRY
                     KKCDCGIMVTASHNPPSDNAVKVYWNTGGQVLPPHDQRMIEAVMGVRAIRRVPFAEAV
                     KNGQVVFCQDEVDAAYFEGLRRQSHEGPRNLSILYSPLHGVGTTAVLPALKNAGFTQI
                     EVFGPHAEPSGDFPNVPNHVANPENKQVFEPLIARAKEIGADIALATDPDCDRLGCAV
                     PETYERNAAWTTLTGNQIGSLLTDYVIRKAQEAGQLTPQHYVVETLVTTRLLGRIARK
                     AG"
     assembly_gap    4841..4940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5200..6573)
                     /locus_tag="PLD05_06310"
     CDS             complement(5200..6573)
                     /locus_tag="PLD05_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOP77083.1"
                     /translation="MLRTDEILSTIRMLHAEHLDVRAVTLALNVEDCAAPNVDHMCRK
                     LRDKITSRAARLVEICDRVGAKYGIPVVNKRLAISPVSTLLAGHGKGAALQVAKTLDA
                     TATACGVDFLGGFTALVHKGISPGDQVVMESLPEVLASTQRVCASVNVASRKAGINMD
                     AVREMGFIIHRTAQLTADRKGFGCAKLVVFANIPEDNPFMAGAYMGAGEPEATVNIGI
                     SGPGVVGSALKRRLERGDKVTLGDLAEEIKITSFRVTRVGELIGREVAAELGVAFGIV
                     DLSLAPTPTVGDSIGEIIKTLGIEKIGAPGSTAAVAMLNDAVKKGGLFASSSVGGLSG
                     AFIPVSEDAALAQAVGAGHLVLEKLEAMTSVCSVGLDMIAIPGDTPPETIAALIADEM
                     AIGVINDKTTACRLIPVPGAVAGEWVDFGGLFGAAPVIEVRNSGASAAFIRFGGRIPA
                     PLQSLGN"
     gene            complement(6792..8159)
                     /locus_tag="PLD05_06315"
     CDS             complement(6792..8159)
                     /locus_tag="PLD05_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77084.1"
                     /translation="MSLQRIGQRSRTSLTGQNRRDFLKTSATATAAALTTTSLARFVH
                     AAENNLIQVALIGCGGRGTGAAANALSTQGPIKLVAMADVFQDRLNASLNNLKNSFGD
                     KVDVPPERQFLGLDAYKKAIEALNPGDLVLLATPPAFRPIHLEFAVEKGRHVFMEKSF
                     AVDGPGIRRVLAAGKVAEQKNLKIAGGLMSRHSLPLEQIIERIHDGALGNVISVWAYR
                     MHGPVGFRRRQPGESELAHQIRNYSCFTWLNGSFIVDWLIHNIDVACWVKGDWPVSCQ
                     GQGGRQVRNEPDQLFDHYAAEYTFADGTRMFAQARHMANCWGFWGVVVHGATGSAVLG
                     EGIADPRMYKGYKRVAEDEIWKYEGPPCNPYQRELDLLVDAIRNDKPYNETERCAKSC
                     LAAILGRMAVESGQEVTWEEALQSELSLAPGIDTWKSLDDPAPVQPDENGRYPIAMPG
                     FTKAF"
     gene            8397..9854
                     /locus_tag="PLD05_06320"
     CDS             8397..9854
                     /locus_tag="PLD05_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77085.1"
                     /translation="MTTVLITLLALTTVADAAPRPNVVYIVTDDQRYDALSCAGHPFL
                     KTPHCDQLAQEGARFINAFVTTSLCSPSRASSISGLYAHSHGVIDNFTEFPDDLPSLP
                     KQLQAAGYETAYIGKWHMGEDNDAPRPGFDYWASHKGQGKYFDNEFNINGQRVMLSGY
                     YTHRITELAVDWLKKPRSKPFLLMIGHKAPHTPFTPEPKYEHIFDHIDIPYPPSAFQL
                     EGKPDWVKERLVTWHGIYGPLFGFREKFPDTRPEAQQDFARMVRAYLATIKSVDDSVG
                     EIVATLKATGQFDRTIVIFTSDNGFLLGEYGMMDKRTMHEPSIRIPLVVRYPPVIKPG
                     TIIKEMVLNVDLAPSILDLCGAPPLPKTHGRSWRPLLEGRTEGWRKSFYYEYNYEKQF
                     PYTPNVRGVRTERWKYVHYPTGDGTPDRHKAELYDLQNDPEERYNLIDDPRYQSVLEE
                     LRQELLRLQKETDALPDRMPIDEGIKTELPEQSIR"
     gene            9948..11237
                     /locus_tag="PLD05_06325"
     CDS             9948..11237
                     /locus_tag="PLD05_06325"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77086.1"
                     /translation="MSTHKLSRRYVLKSLATGAVSAVAIPTVIPRSALGDDATPGANE
                     RITVGVIGTGGRSRLLIEQLPSQGQILAVCDCFLERAEQVNAEKGQGRWAVYQDHRQL
                     LERKDIDAVIVGTPDHTRVLVCIHACQAGKDIYAEKPLTLYIREGRVLVNAVRKYKRV
                     FQVGSQQRSMAMNRIACEFIRSGKLGKIRVVQGCNYTGPRFYEGLPEEPIPAKLDWDR
                     WLGQTPYRPYNRQLHYGWMAWWDYSGGEMTNWGAHGLDQIQWALGKDDTGPVDIRPQG
                     SPRDMNAAVHMKYADGTPVRLELSPGPAGGAIFSGENGRIEINRNKFTCNPPDLIKDL
                     PPQEEIDKWRDEVALWQAKYHMENWMNCIRTREKPVADVEIGHRSITIAHLANIARYL
                     GRRLQWDPVKEVFIGDDEANSLVERPQRAGYELPQIV"
     gene            11748..13931
                     /gene="nuoF"
                     /locus_tag="PLD05_06330"
     CDS             11748..13931
                     /gene="nuoF"
                     /locus_tag="PLD05_06330"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOP77087.1"
                     /translation="MKQDVWDALKNLREKAERVYQERMGQGQIFIQVGSATCEHAAGA
                     IEVADEFRKHIAASGRNDIILRRTGCTGRCSREPIVGVFRAGEMPIKYQLVNRELVHK
                     IFTQHVLGGEPLLDHVLDGPIERLSKYEILNCGSLRCGWKGEQICGGPMANQLKAMGV
                     PPDVCRVTSASCFGACSIELAGKCTHILVRPQNVLYRITSLADLEEVVREHIGKGQIV
                     ERLRVKEEPVSQDFFELYGEIAFFNRQTRIALRHNGIIDPTSIEEYFHYRGFEALAKV
                     LAQDDPDWIVDEVTRSKLRGRGGGGFPTGQKWALARKSPEKTRYIICNADEGDPGAFM
                     DRSMLESDPFNVIEGMIIGAFAIGAHRGFVYVRAEYPLAVERVDHAIRMCREWGVLGK
                     NILNSGFDFDIEIRLGAGAFVCGEETALIHSIEGERGQPRIRPPYPTEVGLWGKPTVI
                     NNVETFANIPAIIIYGADWFSRIGTNASGGTKVFALAGKVKQTGLVEVPIGITLREVV
                     MDIGGGCPNDKKVKAVQTGGPAGGCIPASMLDLRVDFDTLNKAGSIMGSGGMIVLDED
                     DCMVDIAKFFMRFSEDESCGKCTPCREGTTRMVEILERITTGKADESDLEKLERLAKL
                     VRKASLCGLGRAAPNPVLSTLTHFREEYLAHVRDKRCPAKKCTALIHYEIDPEKCVGC
                     TACARNCPVECISGERRQPHVIDQSRCIKCGRCFQVCRFDAVMRS"
     gene            14084..15853
                     /locus_tag="PLD05_06335"
     CDS             14084..15853
                     /locus_tag="PLD05_06335"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOP77088.1"
                     /translation="MIAKRRKKITLTIDGNQVTVPEGTTIMQAAEQIGIRIPRLCYHP
                     DLSLAGSCRVCIVEVKDMGHYMASCSSPVWEGMEVRTNSPEIREARRDIVELLLDNHP
                     MDCQTCERDGNCELQNLAYSLGVRERLYEGKRKEFPIEDSSVSVVRNPNKCILCGRCV
                     RVCAEIQGVYNLSQHGRGFYTVVAPAHKANMDDSVCIQCGQCINVCPTAAFLEKSSTE
                     AVWKALADPNLHVVVQTAPSIRAAIGEGFGYPPGTPCTGKMITALRLLGFEKVFDTDL
                     GADLTIVEEAHEFLTRLQTGGPLPMITSCSPGWISFLEKFYPELIPHASTCKSPMSML
                     STLAKTYYADRMNIPPENIFMVAVMPCVAKKYEAQRPEHLMPDGRPYTDAVLTTRELI
                     WMIKCYGIDFTRLPDGQFDAPLGIASGAGDIFGTTGGVMEATLRAASRILTGKKPDRL
                     EFTEVRAVEGLRDTYVAIGERNVHVAVANGLNNAKQVLNKVVSGEEHYDIIEVMACPG
                     GCIGGGGQPYPSENYHVLDPQLLRKRAAALYTIDGSKELRESYENPALEELYETYLGS
                     PGSVKAHQLLHTQYRPRLPRGIR"
     gene            15850..16413
                     /gene="nuoE"
                     /locus_tag="PLD05_06340"
     CDS             15850..16413
                     /gene="nuoE"
                     /locus_tag="PLD05_06340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOP77089.1"
                     /translation="MNPHCASSTNNWPEVKAVAERLLGDDVVQFIEECRKTSEPNSYL
                     IAVLHKVQSKFGYLGASHLDAVAQLMQIPAAKVSGVASFYHYFRLQPRGKFIINLCLG
                     TACYVKGADRLAEKLKEELGIDFGETTSDGMFTLEGARCLGACSLAPVMLINDDMHGT
                     VTPDQIPIILDRYLKKAKQETASKSAS"
     gene            16725..17582
                     /locus_tag="PLD05_06345"
     CDS             16725..17582
                     /locus_tag="PLD05_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOP77090.1"
                     /translation="MHAKSRFGIGVTWLGKVAILSAAFSFLLGLTPAVAEESSALPPV
                     ESLLPPDPLPALPDGKKWQLVWHDEFEGLQIDTTKWEIYGDWKRRDGYWVKEDSFLDG
                     KGHLVIRTKKSGDRYTSGAIATKGKFEPRFGYLVCRCTHPKQPGMWAAFWMFGPGVTR
                     VGDEGRDGTEIDIMEMPYRDGRLTSNLHWDGYGKDHKHAGTTFRMPEILEGFHTYGLW
                     WKPDEYVFDVDGKETWRTSAGGVCQVPLYIKLTVEIGSWAGDIKTAQLPEDWLIDYVR
                     VYQMFDKEP"
     gene            17733..19952
                     /locus_tag="PLD05_06350"
     CDS             17733..19952
                     /locus_tag="PLD05_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOP77091.1"
                     /translation="MIRMPWLFLLIVSGLTQGVVNGEQWKPAPAPLMTPWAKDVTPDN
                     VWPEYPRPQMVRSQWQNLNGLWDYAICPKEQEKPSAWDGKILVPFCVESALSGVGKAV
                     QPTERLWYRRTFEKPKVAADQRVLLHFGAVDWETTVWVNGQEVGRHTGGYDPFTFDIT
                     SALRDGENEIVLAVWDPTDTGWQPRGKQVLNPKGIWYTAVTGIWQTVWLEVVPSQYIA
                     SLKLVPDVDQGLLRVTVNAPTGLGVEVQAFFDGQSVGHAHGTTGSTIELKIPNPKLWW
                     PDEPNLYDLDVKLIQNGQVIDQVKSYFGMRKVSVEKDERGINRLMLNGKFVFQLGPLD
                     QGWWPDGLYTPASDEAMKYDIVMTKKFGMNMARKHVKYECDRWYYWCDKLGLLVWQDM
                     PSGNVNRNEESRANFRRELKAMIDARFNHPSIIMWVPFNEGWGQHDTCDIVAWIKDYD
                     PSRVVNEASGWHDNGCGDISDMHKYPGPGVRPIEEKRACVLGEFGGLGMPVPGHLWRE
                     QGSWGYVAYKDFEALTDAYVRLLTGVRMLIPEGLCAAVYTQTSDVEIEVNGLMTYDRA
                     VTKIDIARAAEAARKLYLPPPVVKTLVPTSQNEPQTWRFTTEQPTGEWFAPDFDDSSW
                     KEGPGGFGTEGTPGTVVRTIWKTPDVWLRRSFELSEVPSEGELWLNIHHDEDAEVYIN
                     GVLVASPKGFLTNYTLMPLAAGRIEDILRRGKNVIAVHCHQTQGGQYIDVGLSHVTE"
     gene            complement(20063..20680)
                     /locus_tag="PLD05_06355"
     CDS             complement(20063..20680)
                     /locus_tag="PLD05_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77092.1"
                     /translation="MVMYRVVVIETPEDWNPSSLDDVPPQPPEPKVQLGEYPELFPAV
                     QAAIEHNRQSQNQRDRRWAVVCEVGQGGKTWRGLRICTPLRYKIASIWWPAGWEPLSP
                     FDVPLCVCRTQGTLQEDVLTYDQALATMKALNLQAMDRASTLWYVMIAVENEPVSRSI
                     SYDPAGLQTTVEIRKIHIAQPAEGGRGDCSHCPARSLDCTTAANQ"
     gene            21036..23954
                     /locus_tag="PLD05_06360"
     CDS             21036..23954
                     /locus_tag="PLD05_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOP77093.1"
                     /translation="MKAFHLPCQVNLIRVGTLGSVLFLLATLSTGDSVYGNDNVPTGP
                     VVDVQMTEEMLVADFLRQAELRESKAHRGSAQHPSEDARGGCDGIKNGQWGFHTNREP
                     QPWWQVDLGREYPITKVMIFNRCDTVAHRASRLHVLVSGDGQSWTKVYEHDGTVFYGQ
                     TDGKPLVVDLPGVAARFVKIQLPDTEYLHLDEVEVYSSEDQEQANIALGKRADQSSLS
                     QWSVYHPEVPQEGPIFPLEKVLERGLALAEELLEMPSLVEPARGIVEREKAYLKELAS
                     RVARSKLSSEERRQLYCDACLAVRRMSWQNPLLDFRDLVFVKRKPPIFHHMSDQHYGW
                     WCQGDGGLFILEDWKSEHPRLRCLTASSPSGSVLRPDISYDGRRILFAYARYYPGLHQ
                     LANKLDKNNVPEDAFFHIYEINVDGTGLRRLTYGKYDDMDARYLPDGRIVFLSTRRGR
                     QTQLLPRPVNYPGESANPDCYVRCGGGPERPVAIYTLHVMDGNGENIRPISPFESFEW
                     HPSVDFSGRILYARWDYVDRHNQPFMGLWATLPDGTGVQAIYGNFTWNPLSIFEARAV
                     PGSHKIVFTASAHHSITGGSLVLLDPRRGFDDEAPLTRLTPEVCFPESEGWPLHYYAN
                     PYPLSETFMLVSWSDQPLADHAGRMSDAGSGIYLYDTFGNLNLIYRDPTIGCETPLPL
                     KPRAKPVVASAETTETSAEEGYFVVSDVETGMTGIARGTVKRLRLVGIPVKTHPTMNY
                     PELGLTREDPGKFVLGTVPVESDGSAYFRVPAGVSLFFQALDEKGMAVRTMRTAVYVQ
                     PGQVVSCVGCHEPRNSTPLNKPIAALNREPSLILPGPEGSWPFDYDRLVQPVLDRHCV
                     KCHAPGEKAAAFDLTPGASYKTLVNYGHPSLAEAVATAYRRGRSLPGEGLARMNPLWV
                     VLEKGGRISELTAEEKERLIIWMDLYGQRRGYFDEQQYQELLQLREKWKALLATGAAE
                     "
     gene            24018..25277
                     /locus_tag="PLD05_06365"
     CDS             24018..25277
                     /locus_tag="PLD05_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOP77094.1"
                     /translation="MQAEVIANGDEITSGRILDTNSQWLSIRLEDLGIRVMYHTCVGD
                     DLQAMVAVFRQAIERSDVVVTTGGLGPTADDLTREALANVTGRQLIELPEALEHVRRF
                     FAQRQRPMPERNRIQAAFPVGSEMIRNPNGTAPGIAMTIPREDRSPCHFFCLPGIPSE
                     VYEMWPEVEERLRKIGAGKEYILHRDIKCFGAGESQVESMLPDLIRRGNDPLVGINAS
                     KNTIILRISTKGPNRQACEEKILPTVEIIQQCLGDLIFGYDEEELQDVVLARLSELGK
                     TLAIVETDTSGMVADWLYQSKAQSQGKPFRGGIVLASEDSVHRIVGSPSASSGQPVPS
                     DRQGLTVLLASHVRTLFGADIGLAVSSLKPTELSRVVSFAVGVVSGETSFQQEIRFGS
                     HPDFVRLTMGKHMLNTLRLWLKGKLTS"
BASE COUNT         5688 a         6836 c         7180 g         5649 t
ORIGIN      
        1 ggcccggcgg tggaaatcaa ccctcactcg tttcgttctc cctcacccgg ctcgctggcg
       61 ctggttaccc tctccccgag ggaaaggccg aaccacactg ttccatccgt cacacggaat
      121 tgcagctatc attttggttg acataggcac cgcgcatttt gatggcgact tcgaatcttc
      181 cttgttctgt gaggcgagtg gaggttactc ttcaagatga acctagcttg ctctgtgaga
      241 tgtacgcaga ttaccctgcg aggtgaaccg cggtcattgt cttcgtctat cgcttttgag
      301 ggattttggc cgatagctgc gtgccgacca ggtctgtgac ctgccctttc accattatgg
      361 cgaccgtaac tatcggcatt tccgccctcc gaagttgcca gtccaatacg tggtgatcta
      421 tttatggtag gttcagtcgc aacggtcagt tgcttgcacg tcgggcggtt ttccgcataa
      481 caggctctat gaagggtagc gctgactggc accccaggcc aagccgtccg ctttcggttg
      541 tacttttatc gctgttcggc gtgcgggtac taccaagact ccgaatcgct acgtgtgccg
      601 aatgcccgtt caacccttaa gctccacatg gcccatgggc tcgtaaatgg gacctgtggg
      661 atgaagttcg ctggaaaaga tcacgacctg gtccaccaca cttgggccaa agtcggtatc
      721 ctcgtgttcc cgcaacatgg cgacgaggtg ctcaatggcc cggccacttt ttctcacccg
      781 accaagggtt aggtgggcat ggaagcgtcg ggcttcacgg cgaaagccta ggccactcaa
      841 ggcatcttcc accctttcgc tgagggccgc catttcgctg gcccccagct ccacacctaa
      901 ccagatgacg tgaggccgac tgagtgaggg aaatgccccc gtacccttaa tctcgatgga
      961 aaagggctca acatcggcga gcgactgctc gatggcgcgg cacacgtgat aaacatcttt
     1021 atattccaca tcgccgagaa atttgattgt cagatgcata ttctctgggg ccacccaggc
     1081 tacatccgcg cccgattggc gaagttgctt gatgaacttg gcagctcggt tacggacggc
     1141 gtcatcaatt tccactgcca caaatgtccg caccttgttt ttcatggtac gtcttcccct
     1201 gaacccatcc gctacctttt catatctaga acctatctca caacctccac tcacccgttc
     1261 ggcgggagac gggtcggggg cgaggagctc aaatcgcccc cggttttctc aatcgattcc
     1321 gatgttcgct tgccctcacc ccaaccctct cccggaggga gagggggann nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cagagttaca caatggcctt cattcagtac
     1501 gcctatgctg caaactcgac cttaactcgc tttttcagag cctctgagcc tttgactaat
     1561 tttacccgtt tcacaccaaa gtaatgagct tcctcatagt cctttcaaga cgttggcaac
     1621 agttccgcac agttgcttag cttcttcctc aaggggtgct tctgcgatga tcctgacgat
     1681 cggctcggtg ttactaggtc ggagaagcac ccatcggtcg ggccagtcca gcctcagacc
     1741 gtcgagacgg tccgcccgag cgtcgcgaaa ttgacgcatc acggcatgat aggcggcctc
     1801 aagtttctcc gtaggaaatt ccactttcgt cttgacaatt gcgtaacgag gcaggttggc
     1861 cgccaattcg gcaacggtgc accccgtact cgccatagca tccaggacgc acgccatacc
     1921 cacaaagcta tcccgaacta ggacgacccg cggatcgatg ggacctccgt taccctcgcc
     1981 accgaagacc gcatcgtgag caatcatggc atccacaacg ttggcctcgc ccacggcgct
     2041 gcggaaaaaa ggcacgccgt atttttgtgc gatgtcctga ttcatccggc tactagcgca
     2101 attggtgacc accggaccct tgcgagtctt aagacgatga tcgagacaca aagagagggt
     2161 gtattcttcg ccaaggtatc gtccggacgc atcgatgatg gctagccgat ctccatcggg
     2221 atcctggcag aacccgatgt cggcttggca ttgccgtacg gcatcgcaga tactcaggag
     2281 attctcggcg agcggttccg gcggatgctc aaaggccccg ttgggaattt caccgcgggg
     2341 caccacttca cagccaaggg cttctaacaa gggcaatcca agcacactgc cggcaccgtg
     2401 atttgaatcc aggaggaccc ggaaatggca agcccggatg cggttaacat cgacggtctt
     2461 aagaatcgca ttcaaatggg gagtaaccgt atcgggacag atttcacgag tcccgacgcg
     2521 ggccgctcgt acccattcca aacgcccttc gcgatagcgt ttgatcacct cttgtccgag
     2581 tgtgcttgga atgacacggc cctcggaact aaacaatttt agtccgttgt attcgggcgg
     2641 attgtggctt gccgtgattt gaatgccacc cgccgcccga taatgctgca ccaaaacacc
     2701 aacggtaggg gtggccgcaa tgttcgcatc cagcacagta cggccagtgc ccagcagccc
     2761 tgcgagcacc gccgcatgga tcatggtccc gctggcccgt ccatcgcggg cgaggacgat
     2821 tgcccctgcc ggtgcagttg cagcccaagc cgcggcaaat cgcatggcca cttctggcgt
     2881 gagactctcg ccaataatac cccgcaagcc agaaacgctg ataatcggtt ctgccattgt
     2941 gcaacctctt tcccaaggaa ccgtggacct cagaagtcca tcttaccccg tcttttgggg
     3001 gttgatcgct tgttacccgc ggcttttgtc cctacaatgc aaaggtcgga tgatagtacg
     3061 tccggtccgt tgcaggaatt ggcgttagat ggtgaaggta ttcgtaatta ttgcgatttc
     3121 gcgggccagg acgaagtaca atcgcaatcg tgatggcgga atcttacaca aactggcggt
     3181 tcagtgtaca gagtgaccgc tgagcaaata ggcaaacagg tagtttgggt agtttgggtt
     3241 attccaagga ggtggacaca caaccagaat gatgcttagc agtcttgggt aacaattggg
     3301 tgcaattgat ctcgcgtttt cgagcacgct gaacacagag aaaggtccaa cgatggcttg
     3361 ttcaggcctt gtcctccgcg agggaaaaag ccggttggtc cggcgttccg cagggttatt
     3421 cctcggcgaa tctcgcagaa ggcatatcgc ttccagaaaa acacccaggt tgttcgctcg
     3481 gtttcctcga acaagtcacc tccaggcgaa agcggggcgc aaatggaaga tctccatttg
     3541 ttttcttagc tagtttccgg ggtggcgctt cccaggctgg acacacacga gcaaaggtta
     3601 gcgtctcttt ggacaggtcc atcacttttc ttaagtggaa aaactctcgg aggcaaagga
     3661 tggtcgtgga gtacgatgtc atccgggata gtctggcgag gctggaggag gcagaacaga
     3721 acaatagcgt ctctccctcg gcagcgcgga acattcgtcg gtggctgacg gaggctgagt
     3781 ttaccgagta tgccccacag attgtggaaa tgattcaagc ggggcgatgg caagagctga
     3841 atgacgtgtt ttggcagatt atcccgttcg ggaccggcgg tcgtcggggt cgcatgtttc
     3901 ctgtggggac gaacgccatc aatgatcgca cgatcggaga gagtgctcaa ggcgtcgccg
     3961 actatgttcg ctcggtcttt ggggagaagg ccctctcttg tgtgattgcc tacgataccc
     4021 gccatcgttc ccggcatttt gccgaactct gcgccgaagt tatggtagct tctggattta
     4081 cggtgttttt cctcgatgga tttcgcagca cgcccgagct atcgttcgct gtacggtata
     4141 aaaaatgtga ctgcggaatc atggtgacgg ccagccataa tccgccttct gataatgcgg
     4201 tgaaggtcta ttggaacacc ggtgggcagg tattgcctcc acacgaccaa cgaatgatcg
     4261 aggccgtcat gggagttcgg gcgatccgcc gggttccctt tgccgaggcg gtgaagaatg
     4321 gccaggtcgt tttctgccag gatgaggtcg atgctgccta ttttgagggc cttcggcggc
     4381 aatcgcatga gggtcctcgc aatttgagca tcctttactc gcctttgcat ggggtaggga
     4441 cgacggcggt cctgccggcc ttgaaaaacg ccggatttac ccagatcgag gtttttggcc
     4501 cccacgccga gcccagcggg gacttcccca atgtccccaa tcacgtcgcc aatcccgaaa
     4561 acaagcaggt ttttgaaccg ctcattgccc gggcaaagga aatcggggct gacatcgccc
     4621 tggcgacgga tccggactgc gaccggctgg gttgtgccgt cccggagacg tacgagcgga
     4681 acgcagcatg gacgacattg accggtaacc agatcgggtc acttttgacg gactatgtga
     4741 tcagaaaggc ccaggaagct ggccagctga cgcctcagca ctatgtcgtg gagacgctgg
     4801 tgaccacacg gttgctgggg cggattgcgc gaaaggccgg nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn cggccttcag gcacggagcc gaaatgtaag ttctatgttt
     4981 tcggttatct cccgccctcg gcccttggcg atttgcacca agctcgagac aatctccagc
     5041 gccaactgaa gttgattgcc gaagacctca aggcattctc gttcgaaggc gtctcgtcat
     5101 cctagtcgta agatgcaagc cagtggtgcg acgctacccg tcgtcctgaa atcggtttcg
     5161 ctccaccgat cagggcaggt tgctttggag tgtattttct caattcccaa gtgactgaag
     5221 tggggcgggg atacgtcccc caaaccggat gaaggccgcg gaggccccgg aattacgaac
     5281 ttcgataacg ggagcagcac cgaacagccc gccaaaatcg acccactcgc ccgccacggc
     5341 gcccggcacc gggatcaatc ggcaagctgt cgtcttgtca ttgatgacgc cgattgccat
     5401 ttcgtcggca atcagggcgg cgatcgtttc tggcggtgtg tctcccggga tggcaatcat
     5461 gtcaagcccg acggaacata cggacgtcat ggcttcgagc ttctctaaga cgagatgtcc
     5521 cgccccgaca gcttgggcaa gagcggcatc ctcactaacg gggataaagg ccccgctcaa
     5581 tccgccgacc gagctggagg caaaaagtcc cccctttttc acggcatcat tgagcatggc
     5641 gaccgccgct gttgagcccg gggccccgat tttttcgatc cccagggtct tgatgatttc
     5701 gccgatactg tcacccaccg tcggtgtcgg agccagagag aggtccacaa tcccaaaggc
     5761 gacgccgagt tctgcggcca cctcgcggcc aataagctct cccacccggg taacacggaa
     5821 gcttgtaatc ttgatttcct cagcaagatc ccccagcgtg actttgtcgc cgcgttcgag
     5881 gcgtcgtttg agggcactac caacaacacc gggccccgat atgccaatat taacagttgc
     5941 ttctggctcg cctgccccca tgtaggcacc cgccatgaag gggttatcct cagggatgtt
     6001 agcgaacaca acgagtttgg cacatccaaa gcctttgcgg tcggctgtca gttgtgccgt
     6061 tcgatggatg ataaaaccca tttctcggac ggcatccata ttgatcccag ccttccgcga
     6121 ggccacgttg accgaggcac ataccctctg tgtgctcgct aggacctccg gcaggctttc
     6181 catcactacc tgatcccctg gcgagatgcc tttgtgaacg agggccgtaa aacccccaag
     6241 gaagtcaact ccacacgccg tggccgtggc atcaagggtt tttgctacct ggagagccgc
     6301 ccctttcccg tgtccggcga gaagtgtcga aacgggtgag attgccagcc gtttattcac
     6361 caccggaatc ccgtacttgg cccccacacg atcgcaaatc tccaccagtc gtgccgcccg
     6421 tgaggtaatc ttgtccctca atttacggca catgtggtca acattggggg ctgcacagtc
     6481 ttccacgttg agcgccaatg taaccgcacg tacgtccaga tgctcggcat ggagcatccg
     6541 aatcgtcgaa agaatctcat ctgtgcgaag catcgttttg tattccggat gatctaccgc
     6601 ttcccaaaac taaagagaac cccgggccga caatcaaccc cggggttttc cgcttcaaga
     6661 actgtattcg tcgcacgttc ggcaaaaacg agctgggcca gcgcaaatag cgctactttc
     6721 ccgcgaagaa acatccccaa ccgtctcccg ggtttcgtga cgcgtgctac taccaaatta
     6781 atgtagcgtc tttaaaaagc ctttgtgaag cccggcatgg cgatggggta tctcccgttc
     6841 tcatcgggtt gaacgggcgc tggatcgtca agactcttcc aagtgtcgat accaggagcc
     6901 agcgaaagct cagattgtaa ggcttcttcc catgtcacct cttggcccga ctcaacggcc
     6961 atccggccca agatcgctgc taagcacgac ttggcacagc gttccgtctc gttgtagggc
     7021 ttatcgttgc ggatagcatc gacaagcaga tcgagttccc gttgataggg attacacggt
     7081 ggaccttcgt acttccaaat ctcatcctcc gcgactcgct tgtatccttt gtacatccgc
     7141 gggtcggcaa tcccttctcc gagcacagca gagcccgtgg ccccatgcac aacaactccc
     7201 caaaatcccc agcaattggc catgtgtcgt gcttgggcaa acatgcgtgt tccatcggca
     7261 aaggtgtact ctgcggcata gtggtcgaaa agctggtcag gctcattacg gacctgccgt
     7321 cctccctgac cttggcagga gacgggccaa tcacctttga cccaacaagc cacgtcaata
     7381 ttgtggatga gccaatccac aatgaaacta ccgttcaacc aggtgaaaca gctatagttg
     7441 cgaatctggt gcgctagctc actctctccc ggctgacgac ggcggaatcc aaccgggcca
     7501 tgcatccgat aggcccagac cgaaatcacg tttccaagag ccccgtcatg gattcgttca
     7561 atgatctgtt ccaacggcag actgtggcgg ctcatcaagc ccccagcgat cttgagattc
     7621 ttctgctcgg cgaccttgcc cgccgcgagt actcggcgaa ttcccggccc atccacggcg
     7681 aatgacttct ccatgaagac atgtcggcct ttttccacag cgaattctag gtgaatgggt
     7741 cgaaatgctg gaggagtggc caaaaggaca aggtcgcccg gattcaacgc ctcgatcgcc
     7801 ttcttgtagg cgtcaaggcc cagaaattgg cgctccggcg gaacgtcaac tttgtccccg
     7861 aagctattct tcagattatt gaggctcgcg ttgagccgat cttgaaacac atcggccatg
     7921 gccacaagct taatgggacc ctgagtggag agggcgttcg cggcggcccc ggttcctcgg
     7981 ccaccacaac caataagggc aacctgaatg aggttattct ccgcagcgtg gacaaagcgg
     8041 gctaacgagg tcgtcgtgag cgcggcggcc gtagcagtcg ccgaggtctt caggaaatca
     8101 cggcggttct gcccagtcaa tgaggtcctt gacctttggc cgatcctctg caaactcatg
     8161 ggccttctcc ttctgaacgt ttggatacaa tcggacggca ccatcacatg gccattgaga
     8221 gaactggcaa cacattgcgt tgcggcaacg cattgaagat aatccaaacg atggtgggcg
     8281 tcaatcaaac gacgtaaccg tcggtggcgt ccttgacttg cgacggatta catcgttgtc
     8341 caagtcatat tgggtaactt ctttccctgt cacgattttt gctggagaac gcaaccatga
     8401 cgaccgtgct cataaccctc ctggcgttga ctaccgtggc cgatgccgca cctcggccca
     8461 atgtcgtcta tattgtgacc gatgatcaac gatacgacgc cctaagctgt gcggggcatc
     8521 cttttttgaa aacaccccac tgcgatcaac tggctcagga aggagcacga tttatcaatg
     8581 cctttgtgac cacctcgttg tgttcgccca gccgcgcatc ttcaatctcg ggcttgtacg
     8641 cccactccca cggggtgatc gacaacttta ccgagttccc cgatgacctc ccaagtttac
     8701 cgaagcagct ccaggcagcg ggttacgaaa ccgcctacat cggcaagtgg cacatgggag
     8761 aagacaatga cgcaccgcga ccgggtttcg attactgggc tagccataag ggacaaggga
     8821 aatactttga caacgagttt aacatcaacg gccagcgggt catgctatcc gggtattaca
     8881 cccatcgtat cacggagctg gctgtcgatt ggctgaaaaa gccccgttcc aagccattcc
     8941 tgctgatgat cggccacaag gctccccaca cgccgttcac ccccgaaccc aaatatgaac
     9001 acatctttga ccatattgat atcccctatc ctcccagcgc gtttcaattg gagggcaaac
     9061 cagattgggt caaagaacga ctcgttactt ggcatgggat ttatggcccg ctgttcggtt
     9121 ttcgggaaaa gttccccgat acgcgtcccg aggcccagca ggattttgcc cggatggtcc
     9181 gggcctatct cgccaccatc aaatcggtgg atgacagtgt gggcgaaatc gtagcaactc
     9241 taaaggcaac cggtcagttt gatcggacga tcgtgatttt cacatcagat aatggattcc
     9301 tcctcggaga atacgggatg atggacaaac gcactatgca cgagccaagc atccggattc
     9361 cccttgttgt gcggtatccg ccggtgatta aacctgggac gatcattaag gagatggtgc
     9421 taaatgtgga cctggcccca agcatccttg atttgtgcgg tgcgccgcct ttgccaaaga
     9481 ctcatggccg gtcttggcgt cccctcctgg aaggccgcac tgaaggatgg cgcaaatcct
     9541 tctattatga gtacaactac gagaaacaat tcccttacac acccaatgtg cggggcgtcc
     9601 gcacggaacg gtggaaatat gtgcattatc cgaccggcga tgggacgccg gatcggcaca
     9661 aagccgaatt atacgacctg caaaacgacc cggaagagcg atacaacttg attgacgatc
     9721 cgcggtacca atcggttctt gaggagcttc gtcaagagct gctgcgttta cagaaggaaa
     9781 cagatgccct tccggaccgc atgcctattg atgaaggcat taagacagaa ctacctgagc
     9841 agtccatccg ttgagctatt gtgctcgtat gggtcaaacc gtttgatgat catcctagtg
     9901 gaagattccc gacacaattt actccttgag ataggaactg tgaaaccatg agcacccaca
     9961 aactttcgcg ccgttatgtt ctgaagtcat tggcaactgg tgctgtttcg gcggtggcca
    10021 ttccgaccgt gatacctcgt tccgccctcg gagacgatgc aacccccggt gccaatgagc
    10081 ggatcacggt tggtgtgatc ggaacaggtg ggcgatctcg gctgctcatc gagcaactcc
    10141 cctctcaagg gcagatcctc gccgtatgcg attgcttcct ggaacgggcc gagcaggtca
    10201 acgccgaaaa gggccaaggc cgttgggccg tttatcaaga tcatcgtcaa ctgctggagc
    10261 ggaaagatat cgacgccgtc atcgtgggca cgcctgacca tacccgcgtg ctggtgtgta
    10321 tccatgcctg tcaggcaggt aaagacattt acgccgagaa acccctgacg ctgtacattc
    10381 gcgaaggtcg tgtccttgtc aacgccgtgc gtaagtacaa gcgggtcttc caggtgggaa
    10441 gtcaacagcg atccatggcc atgaaccgaa ttgcctgtga gtttatccgc tcaggcaaac
    10501 tgggcaagat acgcgtggtt caaggctgca attatacagg gccgcgcttt tacgagggcc
    10561 tgcctgaaga gccgattccc gcaaagctgg attgggaccg atggctaggc cagacgcctt
    10621 atcgtccgta taaccggcaa ctccattacg gttggatggc ctggtgggat tactcgggtg
    10681 gcgaaatgac gaactggggt gcccacggcc tggaccagat tcaatgggca ttgggcaaag
    10741 atgacaccgg gcccgttgat atccgtccgc aaggaagtcc cagggacatg aacgcggccg
    10801 tccatatgaa atacgctgat gggacacctg tcaggttgga gctatcaccg gggcccgcgg
    10861 gcggtgctat tttcagcggg gaaaacggac ggattgaaat caaccgtaac aagttcacat
    10921 gcaatccgcc ggacttgatc aaagatttgc cacctcagga agagatcgac aaatggcgcg
    10981 atgaggttgc cctttggcaa gctaagtatc acatggaaaa ctggatgaac tgcataagaa
    11041 ctcgggaaaa accggtggcg gacgtggaga tcggccatcg ctcgatcacc atcgcgcact
    11101 tggcaaatat cgcccggtac ctaggtcgcc gactccagtg ggaccccgtg aaagaggtct
    11161 ttattggcga cgacgaggcc aatagtttgg tggaacgacc acaacgggcg ggctacgaac
    11221 ttccacaaat tgtctaaaga tagggagact gtgcccaaga ggcccacgtt aactgggccg
    11281 ggcatatttg tgaattttta aagtgccgtg accgtaagtg taagctggcg gccgcttggg
    11341 tgttcggctc aagcaggtca ggttacactc gcggtgggga cgggctagtt catgctgcgg
    11401 gtcagttgac caagctggca tcggcaacgg agggcgtgcc gcgccgaaca acgttgagat
    11461 aggggacgta ctacaaaagg ggacggcgag agcatcgccc atgcttatct atggcgttcg
    11521 tccgaaagac gctacaataa atgcctgttg tatttgatcc cacgcactga tgacatcgtt
    11581 aggaaatcat tgcgagagac gatcattgga ttcgttcttg aaaaagctgc tctctagaag
    11641 ctggatgctc tgtctttttt gggaaaagat ggcgttttct gttcaagatt gtcaagatta
    11701 gccgaatcct ttgtcgcacc gaggccagga ttagtcgagg ctgagccgtg aaacaagacg
    11761 tttgggatgc actaaagaac cttcgggaaa aggccgagcg ggtttatcag gaaagaatgg
    11821 gtcaggggca gatcttcatt caagtgggat cggccacctg tgaacacgcc gctggcgcga
    11881 ttgaagttgc agacgagttt cgaaaacaca tcgcggcctc gggtcgcaac gacattatcc
    11941 tccgtcgaac aggttgcacc ggccgatgta gtcgcgagcc gatcgtgggt gtcttccgcg
    12001 ccggcgaaat gcccatcaag tatcagttgg tcaatcggga gctcgttcac aagattttca
    12061 cccaacacgt cctcgggggt gaaccgttac tcgatcacgt tctggatggc ccgattgagc
    12121 ggctttcaaa atacgagatt ttgaactgtg gttccctacg gtgtggctgg aaaggggaac
    12181 aaatttgcgg cggccccatg gccaatcagt tgaaagccat gggggtcccg ccggatgttt
    12241 gccgggtaac cagtgcaagc tgctttggtg catgcagcat tgaactagcg ggaaaatgta
    12301 cccatattct cgttcgaccg caaaacgtcc tgtaccgcat cacgagcctg gccgacctcg
    12361 aggaggtcgt gcgcgagcat attggaaagg gacaaattgt cgaacgcctc cgcgtcaagg
    12421 aggaaccagt tagccaagac ttcttcgagc tgtacggcga aattgccttt ttcaaccggc
    12481 agacccggat cgcccttcgc cataacggga tcatcgaccc gacaagtatc gaggaatact
    12541 tccactatcg tggttttgag gcgttggcga aggtccttgc tcaagacgat cccgactgga
    12601 ttgtggatga ggtgacccgt tcgaaattgc gtgggcgtgg tgggggtggc tttcccacgg
    12661 gtcaaaagtg ggcgttggca cgcaaatctc ccgagaagac ccgctatatc atttgcaacg
    12721 ccgacgaagg cgatcccggg gccttcatgg atcggagtat gctcgaatcc gatccgttca
    12781 acgtcattga gggaatgatt atcggggcct ttgccattgg ggcccaccga ggatttgtct
    12841 atgtccgggc ggagtacccc ctggccgtgg aacgggtgga tcatgccatc cgcatgtgcc
    12901 gcgaatgggg agtcttgggc aaaaacattt tgaactcggg tttcgacttt gacattgaaa
    12961 tccggctcgg tgccggggcc tttgtctgcg gagaagagac cgcgctcatc cactccatcg
    13021 aaggtgagcg aggacaacca cgcattcgac ctccctatcc caccgaggtt ggtttgtggg
    13081 ggaagcccac cgttatcaat aacgtggaga cattcgccaa catcccggcg atcatcatct
    13141 atggggctga ttggtttagc cgaatcggta ctaacgccag cggtgggacc aaggtgttcg
    13201 ccttggctgg aaaggtcaag caaacagggc tggtcgaagt gcccattggg attactctcc
    13261 gtgaagtcgt catggacatt gggggtggct gccccaatga caagaaggtc aaagctgtcc
    13321 aaacgggtgg gccagcaggc gggtgtatcc cggcctccat gctggatctg cgggtcgatt
    13381 tcgatacgct caacaaagcc ggctcgatca tgggctccgg gggcatgatc gtcctggatg
    13441 aagacgactg catggtcgat atcgccaagt tttttatgcg attttctgaa gacgaatcgt
    13501 gcggcaagtg tacgccttgc cgggaaggca cgacgcggat ggtcgagatt ttggagagga
    13561 tcaccacggg gaaagccgac gaaagcgatc tggagaagtt ggaacggttg gccaagttgg
    13621 tgcgaaaggc gtcgctgtgt gggctgggaa gggccgctcc caaccctgtg ctgagcacat
    13681 tgacccactt tcgggaagaa tacctggccc atgtccggga caagaggtgc cccgccaaga
    13741 agtgtacggc cttgattcac tatgagattg atccggagaa gtgcgtgggc tgcacggctt
    13801 gtgccaggaa ttgccccgtg gaatgcatct cgggtgagcg gcgacaaccg catgtcatcg
    13861 atcaaagtcg ttgtatcaag tgtggacggt gtttccaggt ttgtcgcttc gacgcggtta
    13921 tgcgatcttg agcagcaaat cgtaaaacga ggtacttcct cggggtgttt gcaaggcttg
    13981 gtgatatgct gagcagattg agcttgtaga aatgtctcgc tgattcgtaa gcgtcaagat
    14041 ggtgttaaca ggtcgaaaac actaggctgc ggacaggcga tccatgattg ctaaacggcg
    14101 aaaaaagatc actttgacaa ttgatggtaa ccaggtaacc gttccggaag gcaccaccat
    14161 catgcaggcc gcggaacaaa ttggaatccg tattccgcga ttgtgttacc acccggattt
    14221 gagcctcgcc ggatcgtgtc gcgtctgcat tgtggaagtc aaagatatgg gccattatat
    14281 ggcctcgtgc agctctccgg tttgggaagg gatggaggtc cgcaccaatt ccccggagat
    14341 tcgggaggct cgccgcgata ttgtcgagct acttctcgac aatcatccca tggactgcca
    14401 aacatgcgaa cgggacggca attgtgaatt gcagaacctg gcctattcct tgggggtccg
    14461 tgaacgactg tacgaaggca aacgcaaaga atttcccatc gaagactcga gtgtttcggt
    14521 ggtccgcaat cccaacaagt gtattctgtg tgggcgttgt gtgagggttt gtgcggagat
    14581 tcagggtgtt tataacttga gccagcacgg ccgaggtttc tacaccgtgg tagccccggc
    14641 ccacaaggct aacatggacg attcggtttg catccagtgt ggccagtgca tcaatgtttg
    14701 cccgacggcg gcctttctag aaaagtccag tacggaagcc gtgtggaagg ccctggccga
    14761 tccgaacttg cacgtcgttg tgcagacagc cccttcgatc cgtgccgcca tcggcgaagg
    14821 gttcggctat ccgccaggaa caccgtgcac gggcaagatg atcaccgcct tgcggctttt
    14881 ggggttcgaa aaggttttcg acaccgacct cggggccgac ctgacgatcg tggaggaagc
    14941 ccatgagttc ctgacacggt tgcaaactgg cggcccactt ccgatgatca catcgtgttc
    15001 gccgggctgg atcagcttcc tggaaaagtt ctatccggag ttgatccctc atgcctcgac
    15061 gtgtaaatcc ccgatgagca tgctttccac cttagcgaag acatattacg ccgatcggat
    15121 gaacatccct ccggaaaata tcttcatggt ggccgtgatg ccgtgcgtgg ccaaaaagta
    15181 cgaagcccaa cggcccgaac atttaatgcc cgacggtcgc ccctataccg atgcggtgtt
    15241 aaccacgcgg gaactgattt ggatgatcaa gtgctacggg atcgatttca cgcggcttcc
    15301 cgatggtcaa tttgatgccc cattggggat tgcttcggga gcaggtgata tctttggaac
    15361 gaccggcggg gttatggagg cgacgctgcg ggcggcctca cggattctca ccgggaaaaa
    15421 gcctgatcgg cttgaattca ccgaggtgcg ggccgtggag ggcctccgcg atacctatgt
    15481 cgctatcggt gagcgaaacg tccacgtggc cgtggcaaat ggcttgaaca atgccaagca
    15541 ggtcctcaat aaggttgttt ccggcgaaga acattacgac atcatcgaag ttatggcctg
    15601 tcccggagga tgcatcggcg gcggcggaca accgtatcca tccgaaaact atcacgtgct
    15661 cgacccgcag ttgttgcgaa agcgggctgc cgctctttac accatcgatg gttccaaaga
    15721 attacgcgaa tcctacgaga atccggcgct cgaggaactc tacgagacct atctcggaag
    15781 tccgggatcg gtcaaggccc atcaactctt gcatacgcaa tatcgaccaa gattaccgcg
    15841 agggatccga tgaatccgca ctgtgcttca tccacaaata attggccaga ggtcaaagca
    15901 gtcgccgagc gtctgttggg cgatgatgtg gtacagttta tcgaagaatg ccgcaagact
    15961 tctgagccaa acagctactt gattgcagtc cttcataaag tgcaatcgaa gtttggatac
    16021 ctgggggcta gccacctgga cgctgtggcg caattgatgc agattcccgc cgccaaggtc
    16081 tccggcgtcg ccagtttcta ccattacttc cggcttcaac ctcgagggaa attcattatc
    16141 aatctgtgtt taggcacggc ctgctatgtg aagggcgccg atcgactggc cgaaaaactg
    16201 aaggaagagc tgggcattga tttcggcgaa accacctctg atgggatgtt caccctggaa
    16261 ggtgcccgct gtctcggtgc ttgcagcctg gcaccagtga tgcttatcaa tgatgacatg
    16321 catggaacgg tcacgccgga ccagattccc atcatccttg accgatacct gaaaaaggcc
    16381 aagcaggaga ccgccagcaa gtcggcaagt taggtcgtct ttaggaccaa ccggtcaagg
    16441 gaaacacatg gcttgaaagc ggcacggtcc tttgtgacaa ttgtcagcgt gctgtttgtc
    16501 gcacgcagaa agtcattttt atccaacgcc tgcgagagaa gaacaatcgt gggaaaaata
    16561 gttcggtttg ctatcttttt cccggatatg cttcacagtg caggtagggc ctagtatctg
    16621 gccaaagcgc aagtaaccgt gtcgtttttc cgcaaggccg ttccacaagc cggtgggacc
    16681 agcatattgt gttggatcga gcagcgaaaa aggagagcca tattatgcac gcaaaatccc
    16741 gtttcggcat cggtgtcact tggctcggaa aggtagcgat cttatctgcg gctttcagtt
    16801 tcctgttggg attgacgccc gccgtcgccg aggaatcgag cgccctgccc ccggtggaat
    16861 ccttgctccc gcccgatcca ctccccgctt tgcccgatgg caaaaagtgg caattagttt
    16921 ggcacgacga gtttgaggga cttcaaattg atacgacaaa atgggagatt tatggcgatt
    16981 ggaaacggcg agatggttat tgggtcaagg aagattcatt tttggatgga aagggacact
    17041 tggtcatccg cacgaaaaag tccggcgacc gctacacctc cggggcaatt gccaccaaag
    17101 gcaagttcga accccgtttt gggtacctgg tttgccgttg cacgcatccc aagcaaccgg
    17161 gtatgtgggc agctttctgg atgttcgggc ctggtgtgac acgtgtcggc gatgaaggcc
    17221 gcgacggaac cgaaatcgac atcatggaaa tgccctatcg cgatgggcgt ttgacaagca
    17281 atctccattg ggacggctac ggtaaagatc acaaacacgc ggggacgacc ttccggatgc
    17341 ccgagattct ggagggcttt cacacctatg ggctttggtg gaagccggac gaatatgtct
    17401 tcgatgtcga tgggaaagaa acgtggcgaa cgagcgctgg aggggtctgt caggtccccc
    17461 tctacatcaa gctgaccgtc gaaatcggct cttgggctgg agacattaag acggcccaac
    17521 ttcccgaaga ttggttgatt gactatgtgc gcgtctatca aatgttcgat aaggagccgt
    17581 gatcggtttt tgcgcaaaag tgatatcgat ttgcgcaaaa gtgcattgcg aagcggggat
    17641 ctcgtagtta ggttgagata ttcaggcaat cgtgcgtggg cacgatatgg tattcaaatt
    17701 ggatgggacg ttgagatgag gagacaacat ccatgatccg catgccatgg ctcttccttt
    17761 tgatcgttag tggcctgacg caaggcgtcg tcaacgggga acaatggaag ccagcgccgg
    17821 caccactcat gaccccatgg gcaaaagatg tcaccccaga taatgtgtgg ccggagtatc
    17881 cgcgtccgca gatggtccgc tcgcagtggc aaaaccttaa cggattgtgg gattatgcca
    17941 tttgtcccaa agagcaggaa aagccctcgg cctgggacgg taagattctt gtgccgtttt
    18001 gtgtggaatc ggctttgagc ggcgtcggca aggcggttca gccgacggaa cgtctgtggt
    18061 atcgccggac atttgaaaag cccaaagtgg cggccgatca acgtgtgctc ctccatttcg
    18121 gggccgtcga ttgggaaacc acggtctggg ttaacggtca ggaggtaggc cgccacaccg
    18181 ggggatacga cccgttcacc ttcgacatca cttccgccct ccgcgatggc gaaaacgaga
    18241 tcgtcctcgc cgtttgggac ccaaccgata cgggatggca accccgcgga aaacaggtcc
    18301 tcaaccccaa aggtatttgg tacacggctg taacgggaat ctggcagacc gtctggctcg
    18361 aagtggttcc ctctcaatac atcgcgtcgc tgaagctcgt tcccgatgtt gaccagggac
    18421 tactccgggt gactgtaaat gccccgaccg gtcttggtgt ggaagtccaa gccttttttg
    18481 atggtcagtc cgtgggccac gcgcatggga ccacaggaag cacgattgaa ctgaagattc
    18541 ccaatcccaa gctgtggtgg ccggatgagc cgaacctcta tgacctggat gtcaaactca
    18601 ttcaaaacgg tcaggtcatc gaccaggtca agagctattt cggaatgcgt aaggtctctg
    18661 tggaaaaaga cgaacggggt atcaaccggt tgatgctcaa cgggaagttt gtgttccagt
    18721 tagggccgct cgatcaagga tggtggcccg acggcctgta cactccggcc agcgacgagg
    18781 ccatgaagta tgacatcgtc atgacgaaaa agtttgggat gaatatggct agaaaacacg
    18841 tcaaatacga gtgcgaccgg tggtactact ggtgcgacaa gttaggtctt ctcgtttggc
    18901 aggatatgcc gtcgggcaat gtcaaccgca acgaggaaag ccgtgccaac ttccgccgcg
    18961 aactgaaggc aatgatcgac gcccggttta atcatccgtc gatcatcatg tgggtcccct
    19021 tcaacgaagg gtggggacag cacgacacct gtgatatcgt cgcctggatc aaggattacg
    19081 atccaagccg ggttgttaac gaagccagtg gttggcatga caatgggtgc ggggacattt
    19141 ctgatatgca caagtatcct ggccccggtg tccgacccat cgaggaaaaa cgcgcttgtg
    19201 ttctcggaga atttggcgga ttaggcatgc cggttcccgg ccacctatgg cgtgaacaag
    19261 gaagttgggg ctacgtggct tacaaggact ttgaggccct cacagatgcc tacgtgcggc
    19321 tccttacggg cgtccgaatg ctcattcccg aaggtttgtg cgcggccgtt tatactcaga
    19381 cttccgatgt tgaaatcgag gtgaacggcc taatgaccta cgatcgggcc gtgacaaaga
    19441 ttgatatcgc tcgcgcggcc gaggcagcca gaaaactcta tttgccgccc ccggtggtta
    19501 agacgctcgt tccgacctcc caaaacgagc ctcaaacgtg gcgtttcact acggaacaac
    19561 cgacgggcga atggtttgcc cccgacttcg atgattcttc atggaaggaa gggccgggcg
    19621 gatttggaac cgaaggcaca ccgggtaccg tggtccgtac catttggaag acccctgacg
    19681 tttggctgcg acgatctttt gaattgagcg aggttccgtc cgagggcgaa ctgtggctca
    19741 atatccatca cgatgaagac gccgaggttt atatcaacgg tgttttggta gcaagcccaa
    19801 agggttttct gacaaactat acgctcatgc cgctggccgc tggaaggatc gaggacatct
    19861 tgcgacgcgg caagaacgtg attgccgttc actgtcatca aacccagggg gggcagtaca
    19921 tcgatgtggg tttaagccac gtcaccgaat gaataccccg gaggcatggc tgagccacta
    19981 actggttgtt ctcagcagtc cgaatgagaa gtgaacatca tttaatcagg atacctgatg
    20041 acatcagtcg ggtatcctgt ttttattggt tcgccgccgt cgtgcagtcg agacttcgtg
    20101 ccgggcaatg agagcaatca ccgcggccac cctcggcagg ttgggcaatg tggatcttgc
    20161 gaatctcaac cgtcgtttga agccccgccg ggtcatacga gatggaccgc gagacgggtt
    20221 cgttctcgac ggcaatcatc acataccaga gagtactagc acggtccatg gcctgaaggt
    20281 taagggcctt cattgtggcc aaggcctgat cgtatgtaag gacatcctct tgcagggtac
    20341 cttgggtccg gcaaacgcaa agggggacgt caaaaggcga caggggttcc cacccggccg
    20401 gccaccagat ggaggcgatc ttgtatcgca gcggcgtaca aatccgcaag ccgcgccacg
    20461 ttttcccgcc ttgtcccacc tcgcagacca cggcccaacg gcggtcgcgc tgattttggg
    20521 attgccgatt gtgctcaatc gccgcctgaa ccgccgggaa tagctccggg tattctccga
    20581 gctggacttt cggctccggt ggctgaggtg gcacatcatc aaggctagaa ggattccaat
    20641 cttccggagt ttctatgacc accacgcgat acatgaccat tgagataacc tcgggaaaga
    20701 tttgaacaat acgtcgcagc gttcaggcgg catcacgctt ggtcgcttcg ttcatggtat
    20761 aggctttcca ggcattgata aagtccccga acaaaggact gactccccgg ggaaaggcac
    20821 ctgccaagtt gtgcttttcg ctcctgtcag gtaatatgtc aagcagtctt ggtgtttatc
    20881 gctgcaggtg tgggacggat gtttaagcgg aggttgcaca ctccggaggt agtaatgccg
    20941 gatccgttgg ggtacggccg attctggaca tataggctgc gagcttccaa ttaattgtag
    21001 gccaggcatt cgctggaagg ctcggagggt ccaaaatgaa agcgttccat ttgccctgtc
    21061 aagtcaacct catccgcgta ggtaccctcg ggagcgtgct tttccttctg gcgactcttt
    21121 ctactgggga ttccgtgtac ggcaacgata acgtgccgac gggccccgtt gtcgatgtcc
    21181 agatgaccga ggagatgctg gtcgccgatt ttcttcgtca ggcggaattg cgcgaatcaa
    21241 aggcgcatcg cggctcggcg caacatccct cagaagacgc ccggggtggt tgtgacggga
    21301 tcaagaatgg ccagtggggc ttccacacca atcgcgagcc ccaaccgtgg tggcaggttg
    21361 accttggcag ggagtacccc atcaccaagg tcatgatctt caatcgctgt gacaccgtag
    21421 cccaccgggc aagccgtctc catgtgctcg tctcgggcga cggtcaatcc tggacgaagg
    21481 tctatgagca tgacgggacg gtgttttacg gccaaaccga cggaaaaccc ttggttgtcg
    21541 atcttccggg agtggccgcg cgatttgtca aaatccaact ccccgatact gagtatttgc
    21601 acttggatga ggtagaggtt tattcctcgg aggatcaaga acaagcaaac atagccctgg
    21661 gcaaacgggc tgaccaaagc agtctcagtc agtggtcggt gtatcatccg gaggtgccac
    21721 aggaagggcc gatttttccg ttggaaaagg ttctcgagcg aggtctggcc ttggccgagg
    21781 aactcctgga aatgccctcc ttggttgaac ccgcccgagg gatcgtcgag cgtgaaaagg
    21841 cttacctgaa agagctcgca tcgcgtgttg cccgttcaaa gctttcttcg gaggaaaggc
    21901 ggcagctcta ctgcgatgcc tgcttggccg ttcggagaat gagttggcaa aatccgctcc
    21961 tcgatttcag ggatcttgtg ttcgtcaagc gaaaaccacc tatcttccat cacatgtcgg
    22021 atcagcatta cgggtggtgg tgccaaggcg atggcggcct gttcatcctt gaggactgga
    22081 aaagcgaaca ccctcgcctg cgttgtctca cggcttcgtc accttcggga tccgtgctgc
    22141 gtcctgacat ctcttacgat ggacggcgta ttctcttcgc ctacgcgcgg tattatcctg
    22201 gtctgcatca attggccaac aaattggaca aaaacaacgt ccccgaagat gccttctttc
    22261 acatctacga aatcaacgta gatggaacag gtttgcgtcg gctgacttac ggcaaatatg
    22321 atgacatgga tgctcgttat ctgcccgatg ggcggatcgt cttcctttcc acccgacgcg
    22381 gacgccagac ccagcttctg cctcggccgg tgaactatcc gggggaaagt gcgaacccgg
    22441 attgttatgt tcgttgtgga ggtggtcccg aacggcccgt cgccatttac acacttcacg
    22501 tcatggatgg aaacggcgaa aacatccgtc cgatttcgcc gtttgaaagc tttgaatggc
    22561 atccgagcgt tgatttttcc gggcgaatcc tctatgccag atgggactat gtcgatcgtc
    22621 acaatcagcc attcatgggg ctttgggcaa ccttgcctga tggaaccggt gtgcaagcaa
    22681 tttatggaaa ctttacttgg aatcccttga gtatcttcga ggcccgggcc gttccgggtt
    22741 cgcacaagat cgtctttacg gcctcagccc atcattccat cacgggggga agtctggtcc
    22801 ttttagaccc ccgtcggggt tttgacgacg aggcaccttt gacccgcttg accccagaag
    22861 tgtgttttcc cgaaagtgaa ggctggcccc tgcattatta cgccaacccg tatcctctga
    22921 gcgaaacctt catgctggtc tcttggagtg accagcccct tgccgatcac gcgggtcgaa
    22981 tgtccgatgc cgggagtggt atttacctgt atgacacctt tgggaatctc aaccttattt
    23041 atcgggatcc aaccatagga tgtgaaactc ccctgccatt aaaaccacga gcaaaaccgg
    23101 tcgtggcgtc tgccgagacc acagagacaa gtgctgaaga gggatatttc gtggttagtg
    23161 atgtggaaac aggcatgacg gggatcgcgc gagggacagt aaagcgtctg cggctcgttg
    23221 gtatccccgt caagacgcat ccgacgatga attacccaga gctagggttg acccgcgagg
    23281 acccaggcaa atttgtccta gggaccgtcc cggttgagtc cgatgggtcg gcctatttcc
    23341 gagttccggc tggcgtttcg ctgtttttcc aggccctcga cgaaaaaggg atggcggttc
    23401 ggacgatgcg gacagcagtt tatgtccagc cggggcaagt ggtatcctgt gtgggttgtc
    23461 atgagccgcg taattcaact ccgctgaaca aacctattgc cgcgttgaat cgcgaaccgt
    23521 cattgatctt accgggccca gaggggtcct ggccgttcga ttatgacagg cttgtccagc
    23581 ctgtcctgga ccggcattgt gtcaaatgcc atgcccccgg tgaaaaggcc gccgcttttg
    23641 atttaacacc gggcgcctcg tacaagacgc tggttaacta tggacatcct agcctggccg
    23701 aggccgttgc tacggcctac cgacgaggac gatcacttcc aggagaagga ctggcccgta
    23761 tgaatcccct gtgggttgtt ttggaaaaag gcggacgcat ttcggagttg acggcagaag
    23821 agaaagagcg cttgattatc tggatggacc tgtacggcca gcggcgaggt tatttcgacg
    23881 agcaacaata tcaagagctt ttgcagctcc gcgagaagtg gaaggcactg ctcgccactg
    23941 gtgctgcgga atagtttgac cttttttggc aagccagacc atacaaaaag tactcactct
    24001 acaaaacgga tgaatctatg caggcagaag ttatcgcaaa cggtgacgaa attaccagcg
    24061 gacggattct cgataccaac agccaatggc tcagcattcg cctggaagat ttgggcattc
    24121 gtgtcatgta tcacacgtgc gtaggtgacg atctccaggc catggtggcg gtctttcgtc
    24181 aagccatcga gcgatcagac gtggtggtga caacgggagg attagggcca accgcggacg
    24241 atctgacgcg tgaggcgctg gccaatgtca cggggcgcca gctcatcgaa ttgccagagg
    24301 ctctggaaca tgttcgacgc tttttcgccc agcggcagcg ccccatgccc gagcgcaacc
    24361 gcattcaagc agcttttccc gtaggtagcg agatgattcg gaaccccaac ggaacggcac
    24421 cggggattgc catgaccatt ccgcgagagg accgaagtcc gtgccatttc ttctgcttgc
    24481 cggggatccc cagcgaggtt tacgaaatgt ggccggaagt ggaagaacgg cttcgcaaaa
    24541 tcggggcagg gaaagagtac atcctccacc gcgatatcaa gtgctttggt gcgggcgaaa
    24601 gccaagtcga gtcgatgctc cccgatttga tccggcgagg taacgatcct cttgtgggaa
    24661 tcaatgcctc caaaaacacg atcatccttc gcatatcaac caagggtccg aatcgccagg
    24721 catgtgaaga gaaaatcctc cccacggtcg agatcatcca gcaatgcttg ggcgatttaa
    24781 tattcggtta cgacgaggaa gaattacaag atgttgtttt ggcacgtctc agcgaactcg
    24841 gaaaaaccct cgccatcgtg gagacagaca ccagcggtat ggtggcggat tggctttatc
    24901 agtcgaaggc ccagtcccaa ggcaagccat ttcgaggtgg tatcgtcttg gccagtgagg
    24961 acagcgtgca ccggatcgtg ggatcccctt cggcaagcag cggtcagccg gtgccaagtg
    25021 accgacaggg attgacggtc ttgttggcca gtcatgtgcg aacgctgttt ggcgcggata
    25081 ttggcctggc cgtcagttcg ctgaagccta cggagctgtc gcgagtcgtt tcgtttgctg
    25141 tgggtgttgt ctccggggaa acatcttttc aacaagagat tcgttttgga agccacccgg
    25201 actttgtccg tttgacgatg ggcaaacata tgttgaatac cctccgattg tggctgaaag
    25261 gaaaactaac gagttgatcg tccgtttctc aacgcccctg ttgcgtatgg cggtgttttg
    25321 agactagaat ctctatcacg gttaacggtt cgaaaccaga agagacgcat ctgaggcggg
    25381 gaccttaaaa gtgaggaacg agttatgtct gaaaaaatgc tgctgagagt tcggctatcg
    25441 gcaatgatgt tcctccagtt cttcgtgtgg gcgtgctgga tggtgcccat cggaacgtat
    25501 ggacccgagg ccctggggct gacgggcgcc cacatgggtt ggatcttttc cac
//
LOCUS       DAOSUT010000050        25373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1277_length_25373_cov_10.178490, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000050 DAOSUT010000000
VERSION     DAOSUT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 25373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25373
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_25373_cov_10.178490"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1049
                     /locus_tag="PLD05_06370"
     CDS             183..1049
                     /locus_tag="PLD05_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOP77095.1"
                     /translation="MNTVRDIAKYDSGSDMTTVVNHLIYDAFGRVTAESNPAVDSLFL
                     FTGRPFDSQTQLQNNLNRWYDPSVGRWLSEDPLGFSAGDANLYRHVRNSQTIYVDPSG
                     LFLETLWDEANIGLGIVSVAKDLYEGQWRQAAVDAGGLAVDVAATLVPFVPGGASAGI
                     KATRATAKAADKAVDAARAVDRVGDAARAARAATGQLHPAISTKVHKALEDHPTLSGA
                     YRRRDPRLVTRGADKQAHYGYQRWHRELDDEVVAWLKRNRDATVEQFEAWLRWRYSQD
                     DLRKRFPGGLPK"
     gene            1046..1969
                     /locus_tag="PLD05_06375"
     CDS             1046..1969
                     /locus_tag="PLD05_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77096.1"
                     /translation="MKRPSVRFHVLHNGPGLFGCDAIQADDAVIGEPPRCEACGHPVG
                     MRAWLPPVRVNFEVCRDGYCDFVFPPVNDILVSSRFKQIYESEGLTGLSGFDPVEIVK
                     IKPKRRAKEPPPAYFRVFVARSQAVVDPEASGFDPPADKPVCPVCRIGSNRKRWKKLV
                     IQPGTWSGEDVFFPRGCTGDIIVSERFKMVCEAHGVTNALLIPVEEYGYDFYPHETKD
                     WDIRKYEETLAVLRTMNRAGRLDDYVEAMEEMRKYVLADPKFDWLEELRERFEGKIDP
                     IGDAAHDAYYNLIAPWYAKYVRKPKGPSRRT"
     gene            2098..3051
                     /locus_tag="PLD05_06380"
     CDS             2098..3051
                     /locus_tag="PLD05_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77097.1"
                     /translation="MRPGCAKEAGRTSGTPSPKQRRQGDSSRRNLRSASATGSADSFY
                     HRQWQDHWWLDAETWASGGTYQNATYDDYNYQYRWTKTWQGQGDGAVSSSASNHVTGL
                     ENGSESGWWTHTVPEGSGSGTGSSSNTSWGSPWSHSYDETITYPGFYDYRYYGRDYGG
                     GSGEEDHIAYVGDVFHESPGSDTGFFPFACSGEDPFFAQAEGGGHYAILVDAVANSTG
                     EQEDLLPRASRRSATSPRAVALPNADRLATEQVFGADARVGHLSRDGTAEPIVTAAEE
                     SGSQQQGADSEDSSAWWWLNPWSYPGPRHIIKEPLHNSHVL"
     gene            3114..4073
                     /locus_tag="PLD05_06385"
     CDS             3114..4073
                     /locus_tag="PLD05_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOP77098.1"
                     /translation="MANREQLTFASARFDVYRRPTRRDRFLQQMGKAMPWDSLIGLIA
                     PFYPKEGQPGRPVTPLVLMIKIYFLQIWFNLSDPEAEELMHDSLAVQRFLNLDLGRDR
                     PPDETTINRFRNLIKEHKLGEKILKTVNEHLERAGITIKKGTIVDATVISAPTSTKNE
                     RQSRDPEMGSTKKGDQWYFGAKLHVGVDSRTKVIHSVSLTPANVHDSQMLSELLHGGE
                     RRVYGDKAYVGQKEAIRKKAPRARRNAPLGEKELRSNRWKSRIRSRVEHVFGVIKHIF
                     GWVKVRYRGIEKNLQYAYGVAAAVNVYIHRRVLSEKTAALSPA"
     gene            4471..5487
                     /locus_tag="PLD05_06390"
     CDS             4471..5487
                     /locus_tag="PLD05_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77099.1"
                     /translation="MFECKIRVRVVLLCLVTLQGVISWLTIHADEKTQSDNQLLEQVC
                     ACLLDLEKSYHRIKITGVERYEAGPVGEYVESLVTFWNRDAMRRLDREIIGPVNDRRI
                     GTVQAAVIKPRLAFEVQKAPKAENYVVTAMGPTPIPFELEADFRWYGKFILAPVSVGE
                     FALSKLLHSPDVLLADVSWREFDNSKCLVLDLRRDAPQGFVKGEVIVDPKLSFAVRRW
                     ELEMRGGYRGVCELEYTTRFGQIPIVEWGIIEWKKASDGSVVERYEWALKDVAFDELS
                     DEVFSMHAFGLPESRPIYRLPWLWAVHVILFAALVSALILVRYFRRRQWVRDEADEGP
                     ASSI"
     gene            6452..6901
                     /locus_tag="PLD05_06395"
     CDS             6452..6901
                     /locus_tag="PLD05_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77100.1"
                     /translation="MTRRRIFWLPAGVCVSVLVGMALFVWAGPCYQDHIEDSAEWGCQ
                     LWPVIRPDGVAACPSDFNSYEVPGAPSCRDGIGSRNIVVGTYGNMPSGRMEQVYGCQW
                     CYIYFACEPEGPFLYGNRWTYHCSIVQRFDRGGQWCNMHSSGSMCPQ"
     gene            6910..8073
                     /locus_tag="PLD05_06400"
     CDS             6910..8073
                     /locus_tag="PLD05_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77101.1"
                     /translation="MGKKFMKPWAKGTDTTHVRLMVRFVFSCILLASFVGVIAKTGKC
                     MAETELEAQLLRAVSAAHDRLVQSTEHVAGELKIIRKEGDASREETIRFAKDGPKLRL
                     EYSRLQGPPQAGPEWAKKERSHIVREIFIHSGKRFYHLEGYLNENSGVLYYSDFEDAE
                     FRVRCNSDFLYWLTILYRFPETSVLDLLQRPIAKVETRPWREVPNALWITGKPMVSEE
                     GTETDWTVVLNPDLLYAAVFYEFQARNATGGVSLRTSCEIEPQRLGEDTLLPRRVLRR
                     AENPGKTPTEWVYQFNFTSVGQVDPSLFEEESLKTLLGTIARVEVLPDGRRVTAGVFH
                     NELPLRAKSPEQRVISRRSVTISVITVGSIIVLLLFVIGSRVKKGSKRLHMKE"
     gene            8142..9170
                     /locus_tag="PLD05_06405"
     CDS             8142..9170
                     /locus_tag="PLD05_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOP77102.1"
                     /translation="MDFIYGTLFIWRLATLSCVILSAGCGRVSGAPGGIYASPAVVDA
                     GKVNLAKQTEVSGDFRVVNSTSRIVKIVDTEVSCGCTRLMLSATSIAPHSSIRVQLVA
                     DLSDGQVGRRTFKALLITDSKSVPAVALELACHVTAEKRAGSVPINLGTLGPGEAFEQ
                     IRSVVSGRAKALRVAAIDAPDEVRRHLTVEPRCIADRMELIIRGRAPEKPGSFTIRVD
                     LLAEGADWEVAQIELQGNVEKDLLLPPVIYMGFVEPGSSSEREVSLKVRPGFQCQARL
                     QQVVLGMAEPLLSVSIGNPGPSDPSLRFALRHPGTAGVFERPLKIILVSKEGKTYELH
                     TEVRARFL"
     gene            9393..9821
                     /locus_tag="PLD05_06410"
     CDS             9393..9821
                     /locus_tag="PLD05_06410"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOP77103.1"
                     /translation="MTTARTMITDEQWEKIAGSFDPPAKAGQSRRDRRVIVEAILWIL
                     RTGTAWRDLPSEFGPWQTAYRYFRTWSKSGLWQRIFYALRALGIEAGEVGVEAWHVDG
                     TVVRASRAAAGGGEKGPRGACRPCVRPVARRVLHQGAHRV"
     gene            9769..10233
                     /locus_tag="PLD05_06415"
     CDS             9769..10233
                     /locus_tag="PLD05_06415"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOP77104.1"
                     /translation="MLGRSRGGFSTKVHIVCDNQGHALEVVVSGGQVHESQMLEPLLA
                     KTEAGLTDEQGVLAAWPVALCGDRAYPARWIDELLGVWEIRPVIPGKLNERRERSPRF
                     FDRVLYRCRNVVERLVGWLKENRRLCVRHEKLATHILSMIYLALLHRYLKVL"
     gene            10636..11565
                     /locus_tag="PLD05_06420"
     CDS             10636..11565
                     /locus_tag="PLD05_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77105.1"
                     /translation="MSGLVGEVENAGRSLSRDGRRSHPWDGLRVAVACLLLVAAGLKA
                     YQSFGGKVLVANSIFEHRWFILGQTTGEVGLALWLVTGWYARVVRWLLAGLFFVFAEY
                     SLYRAAQGKASCGCFGAISVTPWLTFLLDSVVVTSALWVKEVSTVKWPRKWSTWVCLM
                     AGGTALWVGALVGASRLWPVEMAQDLGVRIGDRRILLQPDAWVGRTFPLLSYMSGQPK
                     EIQHGRWLVVLMNPGCGRCWMQLQAYEERAEKEGTPLVVVWLSDWRSVISDGEVKVKE
                     GTFWTTLEPMVEWLGETPVAIRLVDGRVESVEH"
     gene            12150..13127
                     /locus_tag="PLD05_06425"
     CDS             12150..13127
                     /locus_tag="PLD05_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77106.1"
                     /translation="MDNKTHARIIEQLLDNIRKCESVTFTAVNTENLEYPDGRRVEIR
                     TVLRYWGKGDKFRAEVIDGTKGDAATPTFVTAYDGEKHQSVRHDLPSLGYSKESHLVN
                     PSITVNPLLLAFGWLHNPRSPVTWGEIRRRETWETAFARAVQRGRAVRENVECEIVDF
                     PGLIPNTINRVYFAREFRFFPMAAESIDQRSNEVFYNQHVSLYYVEEERVWIPAVIQT
                     SFDAAKLSHKVGGAVKSECVYRITPESVAVNIPLDDSLFILRAPPGGIVTDIDKVKKL
                     ANSDRVGKTSGRHFESSWTFLIGNVFLLGLVCVLLYVRWYWYRKRKVTT"
     gene            13296..14186
                     /locus_tag="PLD05_06430"
     CDS             13296..14186
                     /locus_tag="PLD05_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77107.1"
                     /translation="MSRANGERATHDRLYAVTQRLAPLRTIDLDKLSEKQKVLLRIPV
                     KTQEIRDFLGEPEAWENAEISQVQLVLRFLIVQMDVAIADGQWGLAKQTVLDLVTFAQ
                     TLSRTGDVTHFEKAVTCCRAAARGVEQVLPHISPDEIRQLEKTLDDLKTQWGAVTQVV
                     EAYRRRDTNRDPNLAELQEGIGTYDVLRKRLETVAKKCQAFIVCAMAACRIYEKNHPM
                     GDCSHVSTRVQNDAILNDLIDPFSGTGLRKKISSAGLVVYSVGPNLRDDGGVTGGDPG
                     DIEATDTPDDIAVTVRCGTE"
     gene            14503..14901
                     /locus_tag="PLD05_06435"
     CDS             14503..14901
                     /locus_tag="PLD05_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77108.1"
                     /translation="MKTSVATLLTCLVAVCGMILIAWDASWVVGACSDTIVRPNSPSE
                     TTVQACPPAPISRNCPGSGTVVFTGFFQCDQPNAGTACQGAGVPSFTYCKKVCNCLET
                     VNPATGQLICDPSPVCEWFAAEQKREVACH"
     gene            15862..16350
                     /locus_tag="PLD05_06440"
     CDS             15862..16350
                     /locus_tag="PLD05_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77109.1"
                     /translation="MIRSQVAWEIGSKDNPVKMLDMVESDLTRVLSADVWFRSRLRYT
                     RWRNGQVVLRENCQIEALSLNSDVPLQTFALDGISFLEPGTEVFWNRKKPPPYTGRPL
                     IWDGQSIVPDPKYMVGEGARVQPGRVRWDVLIGANVALLAALATLVSLRRYFRLRKSP
                     SK"
     gene            16456..17211
                     /locus_tag="PLD05_06445"
     CDS             16456..17211
                     /locus_tag="PLD05_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOP77110.1"
                     /translation="MERYRERICLASAIVTGLVAAAAAILTFPRAFPTASDNQPDLLK
                     ASPPIVSLGRVTEGVYPVELTLVNQSSESLIVRGAHASCDCTEISLQPVELHPGEGTT
                     LKFRWNTQYRSGLTRGKFVIEYVRKSDPNKALFKLIPLESDVLADFIVSPDQIVFEET
                     RAETVTVRIERVAAEALHIKKAFVGHPAFRIHVDERAEIITVSFDPQKWVREWGLIEM
                     IIQTDSQHRPVVRPPIRVQWRDSQENKEERARS"
     gene            17238..18053
                     /locus_tag="PLD05_06450"
     CDS             17238..18053
                     /locus_tag="PLD05_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP77111.1"
                     /translation="MHRPILSVIIPVYNEAGTIERVLERVIDVWPEDKEIIVVDDGSQ
                     DGTAELLRQWYTQHCSVHGDCRERRNVRVMLLFHDRNRGKGSAIRTGLQYATGHYVVI
                     QDADMEYDPADYRNLLAPLLNEEADVVYGSRRLGERMYSELNCMQKWVQIPRTWANPY
                     YHGVTILNLLVRMWFRLRVTDEATCYKVLPLEVLRAMDLECERFEFCPEVTAKIARMG
                     LRLKEVPIQYRGRSRRQGKKIRFRDGWEAICTLWRYRSWRPSKRPETEEKRCR"
     gene            18058..19902
                     /locus_tag="PLD05_06455"
     CDS             18058..19902
                     /locus_tag="PLD05_06455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOP77112.1"
                     /translation="MMVEEWRVKILRWGREPLEAIWRQRIAWSVILLLLAHSAILAYG
                     AWLHSPGWDELGHLPAGLAHWLYGDFRFYKVNPPLVRMVATLPLLPDPPKIDWESVVV
                     IPNGRPEFSVSGQLFDQGFEQGCWYFTIARWVCLPFSLLGALVVFRWSKELFGTKSAL
                     IALTLWSFSPLVLTNAQMITPDLGATALAVTSQYLFRQWVREPAWERAFSCGIVLGLA
                     LLSKFTLVILPPLWCGLWFATRSWKHNSSFRALFAQLLRETLQLVVVFMIALYIINLF
                     YTCDGSFTRLGDYTFMSRTLSGSSPEEAYTWGNRFSGTFWGQLPVPLPEQYVLGIDFQ
                     KRDFERGYPSYLRGQWKHGGWWYYYLYGLLVKEPIGFWLLFILAIGLAVPFCEFRVQW
                     REELMLWTPAIVVLALVSSQTGFNHHLRYILPAMPFIFVGTARVGGVFTCLPRTFTGL
                     LLRLGVVTAIAWFIVGSVRYSAHSHAYFNELAGGPAHGHDHLLDSNIDWGQDLLPLKR
                     WLDKHPEIKLSGIAYSVPAPFVQLEKIGPGFDRLPPGLPPGCAPEGAHIEPGWYVVFI
                     REMREQNSPYWYFMRLTPESRIGWTAYVYHLTEDKIQEVMRSLTKAKL"
     gene            19962..21518
                     /locus_tag="PLD05_06460"
     CDS             19962..21518
                     /locus_tag="PLD05_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77113.1"
                     /translation="MSRRSFWKVLYASIALGMVLLSAVIVRIPYLATRSVWYDEASSW
                     QMSQFSLPELWYACQLNVHPPLYYLVLKAWSFVWGDSPVALRSLSIACSALAIVGIFT
                     IGWLSGGGNGVKDARDFDNGKLYVAIVAATLMAVNAFQVNAGIEARMYALGTAFAVWS
                     VVATIRVFRSNSTLWWIVLAFLLLSCTYTHHYCLLVAASEVAVLAVYGLMRWLRHSDH
                     SVIRRLGGLVTGVGFGFLPGVLLLHIQLQRVRENYWTVPLGKDVIVKTFSQFWQPIEP
                     ANAYESGLCVFVVLLLCTALTIRHRSIEKCLVSCCAWLPIVLAGIVSSLVTPIWEGRF
                     FRFNEPFFLVVIPLGLQQISQRQTLRWGFAAIVVAVVSYGTWVFWNARQVEQRGGMRA
                     ATEFVLQNSRPCELVLATSNIHYFPAKYYLRGQREIKLLRDAVREFWGHHLIRPRDLI
                     GLAQLERFRERGIWLLGHSSRPREIAWTDDGQDHREIFHYDFGVPQWTVYVIHIQGRV
                     ASSENKQGNK"
     gene            21580..22269
                     /locus_tag="PLD05_06465"
     CDS             21580..22269
                     /locus_tag="PLD05_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77114.1"
                     /translation="MHTKLGEVEAMRWSICWCLVVAISLSMLVSACQHGGLSDSDRLK
                     QAAQQVQLGYTDRIDMGQKSNIGDQDLELLAAVEGLHFLRVDGSRITDEGLAYLVSQK
                     ELIDLYASNTRLSDEGLKILSACSSLETLVIDKTQVTDAGLAYLAELPNLRRLSLWKC
                     FITDDGCRHLAKLPQLQVLSLDETLVTDAGMKYLTGLKNLRRISLWKTRVSDQGVAIL
                     RSALPDLEINR"
     gene            22266..23273
                     /locus_tag="PLD05_06470"
     CDS             22266..23273
                     /locus_tag="PLD05_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77115.1"
                     /translation="MSMNRVRLDRSIGDCRGFTLIEILVVITIIGVLTMLLLAAVQAS
                     REAARRAQCQNNLKQLALAMINHESAMRFYPSGGWGYRWVGDPNRGSGRRQPGGWAFV
                     ILPYLERRDLFEQALDIHDPSELRWCLAQVNAVVVPTFYCPSRRAAELYPILQSPAHY
                     PYNLDLLENAVKIDYAANAGDFLLDVGAGPVSIAQGDHPAYPWPDYPATGVCFLRSEI
                     SIDHVLDGTSNTYLVGEKNIPRLHYDTGDWRGDDQSAYVGDDYDTIRWANASLLPLND
                     VEAENDDARFGGPHPGVVQMAFCDGSVRPIDLSIDALVHQQQANRKDGQVNNTVRSNG
                     N"
     gene            complement(23563..23700)
                     /locus_tag="PLD05_06475"
     CDS             complement(23563..23700)
                     /locus_tag="PLD05_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77116.1"
                     /translation="MKLLGISAWTLMIWTNVRKSRVKTLVAWMTAALRAERISIANLD
                     K"
     gene            23907..24335
                     /locus_tag="PLD05_06480"
     CDS             23907..24335
                     /locus_tag="PLD05_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77117.1"
                     /translation="MHGFRNAVESLWRDLVDSHFDEEHFISGVMRDCGHDATGVSKTD
                     FQEMLNTGSCAAMQEVGWCCNEVLSYYLANHLGSAEFVRALFVALCAVRAIQAGFAHW
                     DFIFDKILMLLVQNMDKTDYHFRMEFLRNILCENSEGTRS"
     gene            24510..24941
                     /locus_tag="PLD05_06485"
     CDS             24510..24941
                     /locus_tag="PLD05_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77118.1"
                     /translation="MIVPLEHGFLRKKVPGVARLLPVLYNHWYKSECLKLLRRRTKDE
                     VEHAWRAAVVPNRVAGVVTRTVRDEYCWLPEALFVPADECFVVFCWWRLALSDGLEAE
                     VCIVEIERELEVGIAEEVIRLLPTLKLGDFCRRISNTLVGR"
     gene            24991..>25373
                     /locus_tag="PLD05_06490"
     CDS             24991..>25373
                     /locus_tag="PLD05_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77119.1"
                     /translation="MPMAIHGNRTLRFIDANQNGQLDQGDTDITQYTWDHRNRLVKVE
                     HRASYAAAVDRVIENAYDYANRWVRRRLDSDGDGHTDQRRIFVYDGNQIVLDFRRTGS
                     GQVQAGDLEWRYLWGPAVDQILAEE"
BASE COUNT         5702 a         5789 c         7651 g         6231 t
ORIGIN      
        1 gacctaccgc ggagcggagg cggtgctccg acttcgcgcc gctcagttcg acggcgactt
       61 cggccctctc tgggatgccc gccttcgcct agctacctaa cttcaccacg aatattcagc
      121 cacacgcgga aaacgttgac aatggggctg atgaaacagt acagtggacc ttggcggacc
      181 acttgaacac ggtccgggac attgccaagt acgattcggg cagtgacatg accaccgtgg
      241 tcaaccacct catctacgac gcctttggcc gggtgacggc cgagagcaat ccggcggttg
      301 atagtttgtt cttgttcacg ggtagaccct tcgatagtca gactcaactt caaaataacc
      361 tcaaccgctg gtacgatcct tccgtcggtc gctggctgag cgaggacccg ctgggtttct
      421 cggcggggga tgccaatctc taccgccacg tccgcaattc tcagacgatt tacgttgacc
      481 cctcggggtt gtttcttgag acactgtggg acgaggcaaa catcggcttg gggattgttt
      541 ccgttgccaa ggacctatac gagggacagt ggaggcaggc agcagttgac gcaggcggcc
      601 ttgcggtcga tgttgcagcg acacttgttc cctttgtccc cggcggggca agcgcaggga
      661 tcaaggcgac ccgagctacc gcgaaagcgg ccgataaggc cgtggatgcg gctcgggcgg
      721 tggacagagt cggagacgcc gcacgggctg cgcgtgccgc caccggacag ttgcatcctg
      781 ccatctcgac gaaagtgcat aaagctctcg aggaccatcc cactttaagc ggcgcgtatc
      841 ggcggcggga cccgcggctt gtgacccggg gcgcggataa gcaggcccat tacggctacc
      901 aacgctggca cagagagcta gatgatgaag tcgtggcatg gcttaagcgg aaccgggatg
      961 cgacggtgga acagttcgaa gcctggctgc ggtggcgtta tagccaggat gatctgagga
     1021 aacgttttcc cggggggtta ccaaaatgaa gcgtccgtct gttcgttttc acgttctcca
     1081 caacgggccc ggtctgtttg ggtgtgacgc cattcaggcc gacgacgcgg tgatcggcga
     1141 gccgccgcgt tgtgaggctt gcggccatcc cgtgggaatg cgcgcttggt tgccgccggt
     1201 gcgggtcaac ttcgaggttt gccgggacgg gtactgtgac tttgtatttc ctccggtgaa
     1261 cgacattctc gtctcctctc ggttcaagca gatctatgag tccgaagggc taaccgggct
     1321 ttccggtttt gaccctgtgg agatcgtgaa gatcaaaccc aagcggcgag ccaaggaacc
     1381 gcctcctgcg tattttcgcg tcttcgtggc gcggagccaa gccgtggtgg atcctgaagc
     1441 atccggtttc gatccccctg cggacaagcc ggtctgcccg gtatgccgga tagggtctaa
     1501 tcgaaagcgt tggaagaaac tggtcatcca gccaggaacg tggtcgggag aggatgtctt
     1561 tttcccgcgt ggctgcacgg gtgacatcat tgtctccgaa cggttcaaga tggtctgtga
     1621 ggcccatggg gtgactaacg cgctcttgat cccagtggaa gagtatggtt acgactttta
     1681 tcctcatgag accaaggact gggacatccg gaagtacgaa gagacgttgg ccgtgctgcg
     1741 gaccatgaac agggctgggc gattggatga ttatgtggag gcgatggagg agatgaggaa
     1801 gtacgtgctg gcagacccca agtttgattg gcttgaagag ctgcgggagc gctttgaggg
     1861 aaaaatcgac cccataggag acgccgcaca tgacgcctat tataatctaa tcgctccctg
     1921 gtacgcgaag tacgtgagaa agcccaaggg gccaagccgg cgcacgtgac taagtgctcg
     1981 ttgagatgat ggagtagcct actcccgtct gcctttgggc cagccgacag gtggcagctg
     2041 cagagggcag caagcgagat gcggggtccg gcatgagtcc aagcccgcat ttcgggtgtg
     2101 cggccaggct gtgccaagga agccggccgc acctcaggga caccgtcccc gaaacaacgg
     2161 cgtcagggcg attcctctcg gaggaatctg cgctcggcct cggccacggg ctcggccgac
     2221 tccttctacc accgccagtg gcaggaccac tggtggctcg atgccgagac ctgggccagc
     2281 ggcgggacct accagaacgc cacctacgac gactacaatt accagtaccg gtggaccaag
     2341 acctggcagg gccaggggga cggtgcggtg agcagttcgg cgagcaacca cgtgaccggc
     2401 ctggagaacg gctccgagag cggctggtgg acgcacacgg tgccggaggg gagcggctcg
     2461 ggcacgggct cttcgtccaa caccagttgg ggtagcccgt ggagccactc ttacgacgag
     2521 accattacct atcccggctt ctacgactat cgctactacg gccgggacta cggcggcggc
     2581 tccggggagg aggaccacat cgcctacgtg ggcgacgttt tccatgaaag ccccggcagc
     2641 gacaccggtt tcttcccgtt cgcctgcagc ggcgaggacc cattctttgc acaggcggag
     2701 ggcggcgggc actacgccat cctcgtcgac gcggttgcga attcgaccgg agaacaagaa
     2761 gacttgctac cgcgtgcttc gcgcaggtcg gctacaagtc cacgggcggt tgccttaccc
     2821 aacgcggacc gccttgcaac cgagcaggtt tttggggcgg atgcaagggt aggccatctc
     2881 tccagggacg gtacggctga accgatcgtg acggccgcgg aggaatctgg ttcacagcaa
     2941 cagggcgcgg attcggagga cagcagcgcg tggtggtggc tcaatccttg gtcatatcca
     3001 ggtcctcggc acattattaa ggaacctctg cataactccc acgtattata atggagtgga
     3061 gttttttgcg aaggtaagga cggaggatgc cttaattgcg gggaattgcc cagatggcca
     3121 atcgcgagca actgactttt gcctcggcaa ggtttgatgt ctatcgtcgt ccaacccggc
     3181 gtgaccggtt tttgcagcaa atgggaaaag cgatgccgtg ggacagtctt atcggtctca
     3241 tcgcgccgtt ctatcccaag gaagggcagc cgggacgacc ggtgacgcct ttggtactca
     3301 tgatcaagat ctactttctc cagatatggt ttaacctttc tgaccccgag gccgaggagt
     3361 tgatgcacga ttcgttggct gtgcagcgtt ttttgaacct ggacctgggg cgtgatcgtc
     3421 caccggatga gaccacgatc aaccggtttc ggaatttgat caaggagcac aagttgggcg
     3481 agaagattct gaaaactgtc aacgagcatt tggagcgagc agggatcacg attaagaagg
     3541 gtactattgt ggacgccaca gtgatctcag ccccgacctc gacgaagaac gagaggcaga
     3601 gtcgtgaccc ggagatgggg agcacgaaga agggggacca gtggtacttt ggggccaaat
     3661 tgcacgtggg agtggatagt cggacgaagg tgatccattc ggtgagtttg acgccggcga
     3721 atgtgcatga tagtcagatg ttgtccgagc ttttgcatgg gggagagcgg cgggtgtatg
     3781 gggacaaggc gtatgtgggg caaaaggagg cgattcggaa gaaggccccg cgggcgaggc
     3841 ggaacgctcc tctgggggag aaggagttgc ggagcaatcg ctggaagagt cggatacgga
     3901 gtcgggtgga gcacgtgttt ggggtgatca agcacatctt tggatgggtg aaggtccggt
     3961 atcgggggat agagaagaat cttcagtatg cctatggggt agcagcggcc gtaaatgttt
     4021 acatccaccg gagggtcttg agtgaaaaga cggcagcact ttccccggct tagggcgctc
     4081 cccttgcgcg cccagattgg ccggaggcga taaatcaggc caactgggca agatagaaag
     4141 ttatctggta atagaatcta atcctccagt atcacgcaca atggcaaatc gagatattcg
     4201 caacctattt ggtaattgtg cagaggttcc taaatcatgt accgggtgta actggtcatg
     4261 tgaacctggc acccggtgcg acactgccaa tggggagaac tgtcctgtga actgtatctg
     4321 taccttacgt gctgggggga cttcgtgcga ccttatttat tgagcgtcag gtaacgagcg
     4381 catcactgga cgatagtccg tgtatgtctt gtatctttcg ggatgctgaa aacctaaatg
     4441 gcgggcttgg tggccaggtg gtggacaact atgttcgaat gtaagattcg ggtaagagtt
     4501 gtgttgttat gccttgtcac gctgcaaggg gtaataagtt ggctgactat acacgccgac
     4561 gaaaaaacgc agagtgacaa ccagctactg gaacaggttt gcgcatgcct cttggatctg
     4621 gaaaaaagtt accatcgcat caaaatcacg ggtgttgagc ggtacgaagc cggtccagtg
     4681 ggagaatatg tggagtctct tgtgactttt tggaatcggg acgccatgcg acggcttgac
     4741 cgagaaatca ttggaccggt gaatgacaga cgaatcggca cggtgcaggc cgccgtgatc
     4801 aaaccgcgcc ttgcattcga ggtgcaaaag gctccaaagg cggaaaacta tgtcgtgacc
     4861 gcgatgggtc caacaccgat cccattcgag ctggaggccg attttcgttg gtatggcaag
     4921 tttatactgg ctcccgtctc tgtcggcgaa tttgcgctat caaaactgtt acacagtccg
     4981 gatgtactgt tggcagatgt cagttggagg gagtttgata attcaaaatg tcttgtattg
     5041 gacttgcgtc gagacgcacc tcagggtttc gtaaaaggag aggttatcgt tgacccgaag
     5101 ttaagttttg cagtgcgtcg ctgggagctt gaaatgcgag gcggctatcg tggggtgtgt
     5161 gagctggagt acaccacccg attcggtcaa atcccaattg tggagtgggg gataatcgaa
     5221 tggaaaaagg cctcggatgg cagtgtcgtc gagcggtacg aatgggcact caaagatgtc
     5281 gcatttgacg aattgtcgga tgaggtattc agcatgcatg cgtttggatt gcctgagtct
     5341 agaccaattt ataggctccc ttggctctgg gccgttcacg tgatcttgtt cgcggcgctt
     5401 gtaagtgcct tgattttggt ccgatatttt cggcgccgtc agtgggtgcg ggatgaggct
     5461 gacgaaggac cagccagttc tatctaatag ggaagccgga gtgaatgctt gcccactagc
     5521 agagattcca gacgtaagac atacccagtt ctcagtgaac caggacttgc ggcgtatcat
     5581 aatgagttcc cgcttcaaca gcgcgaagat tcctttgttc ggattcagaa tcgcttagta
     5641 cgtggcggtt gtttggaggt ctcttatccg aaacagcgta aggcgaaata cctggagact
     5701 gattgtgggg agcatctgag gtgccggggt cggtacaatt gtacacatca gcccacgggg
     5761 tgacttaaaa cgacactttc gtgggggttg acacgacaca cccacacata atgatgggca
     5821 gcttataaca aagcggggga atcagcttgt cgggagcttg tcgatgagtg ggttggtggg
     5881 cgaggtcgaa aatgcgggcc gtagtttgag ccgagatggc cgcagaagtc acccatggga
     5941 tggcctgcgg gtggctgtgg cgtgcctgct tctggtcgca gccggattaa aggcttacca
     6001 atcctttggg ggaaaagttc tcgtcgcgaa ttcgatcttt gaacaccggt ggttcatcct
     6061 cggccaaacg accggagaag tgggtttagc gttatggctg gtgacgggat ggtatgcaag
     6121 agtcgttagg tggcttctcg caggactatt ctttgtattt gctgagtatt ctctgtatag
     6181 ggtagcgcaa gggaaggcaa gctgtggatg ccttgacgcc ttctctttct ctgtgtctcg
     6241 ctggttgacc tttgcactcg acagtgtaat tgttatcgtg ttacgccttg caaaacagac
     6301 gacaacggca aggtggccgg tgaaaaaagg acggtgggct ggcctaatca gattggggat
     6361 gcccctctgg gcgacaagtt ttgcagaagc tggttctaat tgtatgtctt gtttgtcagg
     6421 ctagaggtct tagacacagg agatggaggg tatgacgcga cgaagaatat tctggctgcc
     6481 ggcaggtgtt tgtgtgtcag tgctagtagg aatggcttta ttcgtttggg caggaccttg
     6541 ctatcaagac cacattgagg attcggcaga atgggggtgt cagctttggc ctgtgattcg
     6601 gccagatggc gtggcggcat gtccttcaga tttcaactct tatgaggtac ccggtgcgcc
     6661 gagttgccgc gatggcatag gttcacgcaa tattgtcgtc ggtacctacg ggaatatgcc
     6721 cagcgggcga atggagcagg tctatggttg tcagtggtgt tacatttatt ttgcttgcga
     6781 acccgaaggg ccatttctgt acggcaacag atggacctac cattgttcga tcgtccagag
     6841 gtttgatcgg ggtggtcagt ggtgcaacat gcattcatcc ggctctatgt gcccacagtg
     6901 aacttgatat tgggtaagaa attcatgaaa ccttgggcga aaggtacgga cacgactcac
     6961 gttcggctta tggtacgatt cgttttctcg tgtatattgc tggcatcctt tgtaggagtt
     7021 atcgccaaga ctggcaaatg catggctgaa accgagttgg aggcacagtt gcttcgggct
     7081 gtttcagccg cccatgacag actggttcaa agcacagaac atgttgctgg agagctcaag
     7141 ataatccgca aagagggaga tgcgtcacga gaagaaacga tcagatttgc aaaggacgga
     7201 cctaaacttc gcttagaata ttcgcggttg caggggccac cgcaagctgg gccggagtgg
     7261 gcaaaaaagg agcgatctca catcgttcgc gaaattttta tccattccgg aaagaggttt
     7321 tatcatttgg agggttattt gaatgaaaac agcggggtcc tttattattc ggattttgaa
     7381 gacgctgaat tccgggtaag atgtaattct gactttctct attggcttac catactttac
     7441 cggtttcctg aaacgtctgt gcttgattta ctccagcgtc caattgccaa ggtggagacc
     7501 agaccctgga gggaggttcc caatgccctc tggattactg gcaaaccgat ggtttctgaa
     7561 gaaggaactg aaaccgattg gacagtggta ctcaatccgg acctgctgta tgcggccgtc
     7621 ttttatgagt ttcaagccag gaatgcaacg gggggtgtgt ccctcaggac ttcatgtgag
     7681 attgaacccc agcgattagg cgaggacacc ttgctgcctc ggcgagtgct gaggcgggct
     7741 gagaatccag gcaagacacc gacggaatgg gtgtatcaat tcaatttcac ttcagtaggt
     7801 caggtcgatc cgtcgctatt tgaagaagag tccttgaaga cattgctcgg gactatcgcg
     7861 cgggtggagg ttcttcctga tgggcggcgt gtaaccgcag gggtctttca caatgaactt
     7921 cctttacggg ctaaatcgcc tgagcagaga gttatctctc gccgttcggt gacaatatct
     7981 gtcattacgg ttgggagcat tatagtgctt ctgttattcg tgatcggttc aagagtgaaa
     8041 aaaggctcga agcgtttaca catgaaagaa tgattttggc ataacgacct agatgcgagg
     8101 cacatactcc gagtaactct ggctgtataa tgaatatgaa aatggacttc atatacggga
     8161 cactgtttat ctggcgctta gcgacgctga gctgtgtgat tctgtcggca gggtgtggac
     8221 gtgtatcggg agctcccggt ggcatctatg ccagcccggc agttgtcgat gctggcaagg
     8281 tgaatttggc gaaacagacg gaagtttccg gggatttcag ggtcgtcaat tcgacctccc
     8341 ggattgtgaa aatcgtggac acagaggtct cctgcggttg cacgcgactg atgttgtcgg
     8401 cgacgagcat agccccgcat tcctctatca gggtgcaact cgtggcggat ctttcggatg
     8461 gacaagtcgg tcggcgaacg tttaaagcac tcctgattac cgattctaag tctgtaccgg
     8521 cagttgcttt ggagcttgca tgtcatgtca cggcagagaa acgggccggt tctgttccga
     8581 ttaacttggg gaccttgggg cctggtgagg catttgaaca aattcggagt gttgtttctg
     8641 gccgggccaa agctcttcgc gttgccgcaa tcgacgcacc cgacgaagtc cgcaggcatc
     8701 tcacggtaga gcctagatgc atcgcggaca gaatggagct gatcattcgg ggacgtgccc
     8761 ccgagaaacc ggggagcttt acgattcgtg tcgatttatt ggcagaaggc gcggactggg
     8821 aggtagcaca gatcgaactt caaggaaatg ttgagaaaga tctacttcta cccccagtca
     8881 tttatatggg ctttgtggag cctgggagtt ccagcgaacg agaggtgtcg ctcaaagtgc
     8941 gccctggatt tcaatgccaa gctaggttgc aacaagtggt cttaggtatg gccgaaccac
     9001 ttctttccgt gtcaatcggc aatcctggtc caagcgatcc atcactgcgg tttgctttgc
     9061 ggcacccggg caccgcaggg gtttttgaga ggcctttaaa aatcatcctt gtttcaaaag
     9121 aaggaaagac gtacgaattg cacaccgaag tgcgagcccg gttcttgtaa tggtaacttt
     9181 gggcagcctc ctaatcgaat caaaattacg tgtcgattgg gcccagcgac gataacaaac
     9241 gataacggga gtggcggacg agggaagagt caaaccgcta ggctctgtct gaaaaccggt
     9301 cttttcggca tgggagaatc tggtgtcatg cttgtcaatg agaggcgttg tgacaatggc
     9361 tgttcataag tcgactatct tacacggagg gtgtgactac ggcgcggaca atgatcacgg
     9421 acgaacagtg ggagaaaatc gcgggctcct ttgatccgcc ggccaaggcg ggacaatcac
     9481 ggcgagatcg gcgtgtcatt gtggaggcca ttttgtggat tctgcggacg ggaactgcct
     9541 ggcgggattt gcccagtgag tttggcccct ggcaaacggc ttatcgctac tttcggacat
     9601 ggagtaaaag cggactgtgg caaagaattt tctacgcgtt gcgggcattg gggatcgaag
     9661 cgggtgaggt gggagtggag gcctggcatg tggatgggac cgtggtccga gcgtcgcggg
     9721 ccgcagcggg aggcggagaa aagggtcccc gaggagcgtg ccgaccatgt gttaggccgg
     9781 tcgcgaggcg ggttctccac caaggtgcac atcgtgtgtg acaaccaagg acatgctttg
     9841 gaggtggttg tctcgggcgg tcaggtccat gaatcgcaga tgcttgagcc gctgctggcg
     9901 aagacggaag caggactgac tgacgagcag ggagtgttgg cggcctggcc ggtggcgttg
     9961 tgtggcgatc gcgcttaccc tgcccgctgg atcgacgaat tgctgggggt ttgggagatt
    10021 cggccggtga ttccgggaaa gttgaatgag cgtagggagc ggtcaccacg gttctttgac
    10081 cgagtgcttt atcggtgccg gaatgtggtg gagcggctgg ttggctggtt gaaagaaaac
    10141 cgccgcctgt gtgtccgtca cgagaaacta gcgacccata ttctctcgat gatctatctg
    10201 gcattacttc acaggtattt aaaagtacta tagaaaacgg cttttcagac agagcctaat
    10261 agggaagccg gagtgaatgc ttgcccacta gcagagattc cagacgtaag acatacccag
    10321 ttctcagtga accaggactt gcggcgtatc ataatgagtt cccgcttcaa cagcgcgaag
    10381 attcctttgt tcggattcag aatcgcttag tacgtggcgg ttgtttggag gtctcttatc
    10441 cgaaacagcg taaggcgaaa tacctggaga ctgattgtgg ggagcatctg aggtgccggg
    10501 gtcggtacaa ttgtacacat cagcccacgg ggtgacttaa aacgacactt tcgtgggggt
    10561 tgacacgaca cacccacaca taatgatggg cagcttataa caaagcgggg gaatcagctt
    10621 gtcgggagct tgtcgatgag tgggttggtg ggcgaggtcg aaaatgcggg ccgtagtttg
    10681 agccgagatg gccgcagaag tcacccatgg gatggcctgc gggtggctgt ggcgtgcctg
    10741 cttctggtcg cagccggatt aaaggcttac caatcctttg ggggaaaagt tctcgtcgcg
    10801 aattcgatct ttgaacaccg gtggttcatc ctcggccaaa cgaccggaga agtgggttta
    10861 gcgttatggc tggtgacggg atggtatgca agagtcgtta ggtggcttct cgcaggacta
    10921 ttctttgtat ttgctgagta ttctctgtat agggcagcgc aagggaaggc aagctgtgga
    10981 tgttttggcg ccatttcagt taccccgtgg ctgacgtttc ttcttgatag tgtcgtagta
    11041 acgagcgcgt tgtgggtgaa agaggtgtcc acggtgaagt ggccaagaaa atggtccacc
    11101 tgggtttgtt taatggcagg cggcacagca ctgtgggtgg gtgccctcgt gggtgccagc
    11161 cggttgtggc cggtggaaat ggcacaagac ctgggagttc ggattgggga caggagaatt
    11221 ttgctccaac ctgatgcctg ggtgggaaga acatttccgc tattgtcgta catgagtggg
    11281 cagccgaaag agatccaaca cggccggtgg ttagtggttc taatgaatcc ggggtgcggt
    11341 cggtgctgga tgcagttgca ggcgtacgag gaacgtgcag aaaaagaagg gacaccactt
    11401 gtggtagtgt ggctatctga ttggcggagt gttatctcag acggcgaggt gaaagtaaag
    11461 gagggaacat tttggacaac actcgagccg atggttgaat ggctggggga gactccggtt
    11521 gcaattcgct tagttgatgg ccgtgtcgaa tcggttgagc actagtttgt tgggattgtc
    11581 cgggaaggag taacttttta ttcaagtgga ggagtaagcc atgagtgtgc gcgttcgaga
    11641 agctttcata tgtgctacga tttttggtgt tgttggaatg tcaggagtgc tgatgcttgc
    11701 gacagctcgt gcacaggggc catgtacgca tgtgatgaaa ccgtgcgtca cgttctattc
    11761 tctttttggg gaatgttgca tgaatgctgg agggtttcag acgggacaat taccccggga
    11821 ttatgaatca acgggctgca aatatgtgaa cgatccggcg cagtgtgggg ctcgtttcga
    11881 gtttctcgct ccgttctatc cgtgcagccg gtggatcgga tattgtggcg gcactgcttg
    11941 ttcgcctgat tgtaattgat cccccatgac ggcctttggc gggaagctgc ggtttccctg
    12001 tttgtggagc ggatcaagct cgttagaaga ttcttgggga aacccaattg tgaggaggac
    12061 aacccagggc gggagagttt gcacaaatga ccccttttct ctatctgtca tgctttctaa
    12121 tcgctttggc atcagggatg gggtctggta tggacaacaa gacgcatgcc cgcattattg
    12181 aacaactgct cgataatata cggaagtgtg aatcagtcac atttactgcc gtgaacacgg
    12241 aaaacctcga gtatccggat ggccgccggg tggaaatacg gactgttttg cgatattggg
    12301 ggaagggcga taagtttcga gctgaggtga ttgatggaac caagggagac gcggcgacac
    12361 ccacttttgt gacggcgtac gatggagaga aacatcaaag tgtgcgacac gatttaccct
    12421 cgctgggcta ttccaaagaa agtcatctcg taaacccgtc gataactgtg aacccgttac
    12481 tattagcgtt tggatggctg cataatcccc gcagtccagt gacgtgggga gagattcggc
    12541 ggcgggaaac atgggaaacc gcgtttgcga gggctgtgca gcgggggcga gccgttcgag
    12601 aaaatgtaga atgcgagata gttgactttc ccggattgat tcctaacacg atcaatcgtg
    12661 tctattttgc gcgggagttt cgtttttttc ccatggctgc tgaatctata gatcagcgca
    12721 gtaacgaagt tttttacaat caacacgtat cgctgtacta tgttgaagaa gagagggtgt
    12781 ggattcccgc tgttattcag acgtcttttg acgccgccaa gctcagccac aaagttggcg
    12841 gcgctgtgaa aagcgagtgc gtgtaccgga tcacacccga gtcagtggcc gtaaatatac
    12901 cgcttgatga ctcgttattc attttgcgtg cgccgcccgg aggaatagtg actgacatag
    12961 ataaagtgaa gaagcttgcc aactccgatc gcgttggtaa aacgtcagga cgacattttg
    13021 aaagttcgtg gactttctta atagggaatg tgttcttgct tggtttagta tgtgtactac
    13081 tctatgtccg gtggtattgg tatcgcaaaa gaaaggttac aacgtgataa acaatgggtg
    13141 gtcgataaaa ggcaatgttc cagactaagg aattgacgat gtggagggct ctatcaaggt
    13201 ccttgctttc gatcggagtg atggttggca ttggaatggt gctctggggg tgtcgcaagg
    13261 actccgcgag ttctctatcg gttgacaaac gtggggtgag ccgtgcgaat ggagaacggg
    13321 caactcatga ccgactgtac gctgtcacac agcgcttggc tccgcttcgc accatcgact
    13381 tggacaaact gtcggaaaag cagaaggtgc tactcagaat tccggtgaag acacaagaga
    13441 tacgtgactt ccttggtgag ccagaagctt gggagaatgc agagatctcc caggtgcaac
    13501 tcgtgttacg gttccttatt gtccagatgg atgtggccat tgctgacggg caatgggggc
    13561 tggctaagca aactgtactc gacttagtaa catttgctca aacattgagt cgaacgggcg
    13621 atgttactca ctttgagaaa gcagtgacgt gttgtcgagc cgctgcgaga ggagtcgagc
    13681 aagtgttgcc tcacatttcg cccgatgaga tacggcaact tgagaaaacg ttagacgatt
    13741 tgaagactca atggggggca gtaacacagg tggtggaggc ctatcgccgc agggacacga
    13801 atcgagaccc gaatttggcc gaattgcaag aaggaattgg gacctacgat gtgctacgaa
    13861 agcggttgga gactgtggcg aaaaaatgtc aggcttttat tgtttgcgcg atggcggcct
    13921 gtcgcattta cgagaagaac cacccgatgg gcgattgctc ccatgtgagc acacgcgttc
    13981 agaatgatgc cattcttaat gatctgattg atccattttc gggcacagga ttacgcaaga
    14041 agatatcttc agcggggttg gtggtataca gtgtgggtcc gaatctaagg gatgatgggg
    14101 gcgttactgg aggcgatccc ggcgatattg aggcgacaga cacgccagac gacatagccg
    14161 tgaccgttcg gtgcggcacg gagtagggag gcggggggat cgcatcgctc gtcccctgac
    14221 aaaatggagt gctcacatat cgtcaagagg tgatctaaaa atgcgaatcg cggttggtac
    14281 gctgctccag gacggtgttt tggagaactg tcagctaaag gtctttggac acgcgccgag
    14341 attatgggaa caagtgctga atccgtgatg agctggatag tgagaggaga tcggagttat
    14401 taggagtggt ttagtggttc ttgaaatgag aagccatgag cggttgcgtg cgcctgagtt
    14461 ttagtagaag gtttttgttg ttgatgtgag gaggagttgg ctatgaagac ttctgtggcg
    14521 acgctgctga cctgtctggt tgcggtttgt ggaatgattc ttatcgcgtg ggatgccagt
    14581 tgggtagttg gggcctgctc ggatacgatt gtcagaccaa acagcccgtc cgaaacgacg
    14641 gttcaggcct gtccacccgc acctatttca aggaactgcc ccggaagcgg gacagttgtc
    14701 ttcaccggat ttttccagtg tgatcagccg aatgcaggaa ccgcttgcca aggagctggg
    14761 gttcctagtt tcacctattg taaaaaagtt tgtaattgtc tggaaactgt aaatcccgcc
    14821 accggccagc taatctgtga tccgtcgccg gtgtgtgaat ggtttgccgc tgaacaaaaa
    14881 cgcgaagtag cttgccatta ataaatcgaa gctatcagca tggattcttg aggaatgcgc
    14941 aaccgtcaat gttgaatttg gttcgggcac gatagatctc gcgtggcaag cctaatatgt
    15001 tgcaatggcg aagtcgctat gacgcttggt tggtaatgtt tacgacgcgt gtgcaaaatg
    15061 gctcgcggaa agggcagatt accgcctttg aagcggcttg tgtgacgatt tgagtgggac
    15121 tcagatttgt tttggcggac aacacaagtg ccaatgtgct cctgagtacc ataaaggtgc
    15181 agaaagccaa ggcgaaacag atgtagttcc gggcggagaa gcgccgacat gttcaactgt
    15241 ccgcgggggt tacgaatagg gttggtaatt cttctcagtc ccttgttcat acatgggatg
    15301 tgcaaagcaa gtgaatcttt agatttgaaa cgcgttctaa aggatgtgag agattcccgc
    15361 accaaaatag tatctggggt ggtgcggatc gagcaaggtt ttttggattt ggaacgaaaa
    15421 gtgtctctca ctaatacctg gcttatacat ttctcagctg accggcttcg ggctgaaaga
    15481 gaagcaggag actttcgtga ggtgtggtgt tgggggtgtt acgaagatga cgctttcatt
    15541 gcctatagcg tcgggcatcc atcgctaccc aatggagcca agatggggct gtcaatctgg
    15601 gatagagatg aaatacatcc taggcagttt ttcgttccgc acccaaggtg gctcggatgc
    15661 ttcccgtacc ccattcttca aatgcatgta tgtcatcccg acgtgttttt ttcttcgacc
    15721 gggaaggcaa gcccatcagt agttcgggaa ggtgagggcg agcaagagaa atggacagtc
    15781 cgttggtcgt cggatgtggc ttctgataac caacttccag cggagttttc cattgttgtt
    15841 atgcctcgac gtgggtttgg agtgatccgc tctcaagttg cgtgggaaat tggttctaaa
    15901 gataacccgg tgaagatgct tgatatggtg gagtcggact tgacaagagt cttatcggca
    15961 gatgtctggt tccgctctcg cttgcgatac acgagatggc ggaatggaca agttgttttg
    16021 cgtgagaatt gccaaatcga agctttatcc cttaacagtg atgtgccact tcaaacattt
    16081 gccttagatg gcatttcgtt ccttgagcca ggtacggaag tgttctggaa tcgcaagaag
    16141 cctccaccct acaccggaag accattgatt tgggacggtc agagtattgt tcctgatcca
    16201 aaatacatgg tgggagaagg ggcgagggtc cagccgggtc gtgtccggtg ggatgtgttg
    16261 attggggcca acgttgccct gttggctgct ctggcaactc tcgtatcact acgtcggtat
    16321 tttcgtttgc ggaaatcacc cagcaaataa agtgattcgc gatttgtgct gcagaattct
    16381 tgagctccaa ggtaggcaag acgttacaag agaatttgac aaatttttcc aggccaatgg
    16441 gagtccactg atgctatgga gcgatatcgt gagcggatat gtctggccag tgcgattgtg
    16501 accggattgg tcgctgctgc cgctgctatc ttaacttttc cgcgggcatt cccaacagct
    16561 tcagacaacc agccagattt gctgaaggcc tctccgccga ttgtgagttt aggtcgagtg
    16621 acggaaggcg tgtatcccgt cgagctgaca ctcgtcaatc agtcttcaga atcgctgatc
    16681 gttagaggtg cgcatgcgag ttgtgactgt actgaaatct ccttacaacc ggtcgagctg
    16741 catcctggag agggcaccac gctaaagttt cgctggaaca cccaatatcg ctctggtctc
    16801 acgaggggaa aatttgtcat cgaatacgtt agaaagtcgg atcccaacaa ggcgttgttc
    16861 aagcttattc cattggagtc tgacgtgttg gcggacttca tcgtgagccc ggatcaaatc
    16921 gtattcgagg aaacgcgtgc cgaaactgta actgtgcgaa ttgagcgagt ggctgccgaa
    16981 gcacttcaca tcaagaaagc gttcgtgggc catcctgcct ttcgtatcca tgtggatgag
    17041 agagcggaaa ttattacggt gtcttttgat cctcagaagt gggtgcgcga atgggggctc
    17101 atagaaatga tcattcaaac agatagtcaa cataggcccg tggtcaggcc gcccattcgt
    17161 gtgcaatggc gggattcaca ggagaataag gaggaacgtg cccgttcctg actgttgtgg
    17221 tagttcatga atgtgtgatg caccggccga ttttaagtgt gattataccg gtctataatg
    17281 aggcaggcac gattgaaagg gtgctggagc gggtaattga tgtttggcca gaggataagg
    17341 aaatcatcgt tgtggacgat ggatcgcaag atgggacagc agagcttctg cgacaatggt
    17401 atactcaaca ctgtagcgtg catggggatt gtcgagagcg aagaaatgtg cgggtaatgc
    17461 tgctctttca cgaccgtaat cgcggaaaag gcagcgctat tcgaaccgga ttgcaatacg
    17521 ccaccggcca ctatgtcgtt attcaggacg cggacatgga atatgatcca gcggattacc
    17581 gtaatctgtt ggctccgctc cttaacgagg aagctgacgt ggtgtacggt tcgcggcgat
    17641 tgggagagcg gatgtatagc gaactgaatt gtatgcagaa atgggtgcaa ataccgagga
    17701 cttgggcaaa cccttattac catggagtaa caattctcaa tctccttgtt cgcatgtggt
    17761 ttcgcttacg agttaccgat gaggcgacct gctacaaagt tcttcctctc gaggtgctgc
    17821 gggcaatgga tcttgaatgt gagaggtttg agttctgtcc ggaagtgact gcaaagatag
    17881 ctcgaatggg attgcgattg aaggaggtgc ctattcaata ccgggggaga agtcgcaggc
    17941 aagggaagaa gattcgattc cgcgatggct gggaagcaat ctgtacgtta tggcgttacc
    18001 gatcttggcg accatcaaag cgaccggaaa cagaggaaaa acgatgtcgg tgacattatg
    18061 atggtcgaag aatggcgggt gaaaatatta cggtggggac gtgaacccct ggaggcgatc
    18121 tggcggcaac ggatcgcgtg gagcgttatt ctgctcttat tggcgcactc tgctattctt
    18181 gcttacggcg catggttgca cagtccggga tgggatgagc ttggccattt acctgccgga
    18241 ttggcccact ggctgtacgg ggattttcgc ttctacaagg ttaacccacc ccttgttcga
    18301 atggtagcca ctctccctct tctgcccgat ccgccaaaaa tcgattggga gagcgtcgtc
    18361 gttataccta acggacgacc ggaattttct gtaagcggac agttgttcga tcagggattt
    18421 gaacagggat gttggtattt tacgattgcc cgctgggtct gcctgccgtt cagtttgctc
    18481 ggggcgctgg tggttttcag atggtcgaag gaactcttcg ggactaaatc agctctcata
    18541 gcgctgacgc tctggtcgtt tagtcctttg gtgctgacca atgctcaaat gatcacaccg
    18601 gacctgggag caactgcatt ggctgtgacc tctcagtatc tttttcgcca gtgggtacgg
    18661 gaacccgctt gggaacgggc tttcagttgc gggatcgtgc ttggacttgc gctcctcagt
    18721 aagttcaccc ttgtgatcct tccgcctctc tggtgcggat tgtggttcgc gacccgaagc
    18781 tggaagcata attcctcttt tcgtgcgctc tttgcccagc ttttgcgaga aaccttgcag
    18841 ctggtagtgg tttttatgat agcgctctat atcatcaatc ttttttacac ttgtgatggt
    18901 tcattcacac gacttggtga ctatacattt atgtcccgga cactgtccgg atcatctccc
    18961 gaggaggcgt acacttgggg caatcgattt tccgggacgt tctgggggca gttgccggtc
    19021 ccattgcccg aacaatatgt gctgggaatc gattttcaaa agcgggactt cgaacgcgga
    19081 tatccttcct atttgcgtgg gcaatggaaa cacggtggat ggtggtacta ttacttgtac
    19141 ggacttctgg tgaaagaacc aatagggttt tggctccttt ttattctggc tatcgggtta
    19201 gctgttcctt tttgtgagtt ccgggtacag tggcgagaag agttaatgct ctggacaccg
    19261 gcaattgttg ttttggcact ggttagttcg caaacgggat tcaatcatca tttgcgctat
    19321 attctaccgg ccatgccctt cattttcgtt ggaacagcgc gtgttggcgg agtattcaca
    19381 tgtttgccca gaactttcac cggccttctt ctacggctgg gggtggttac agcaatcgcg
    19441 tggttcatcg taggtagcgt gcgctattca gcacatagtc atgcgtattt caacgaatta
    19501 gcgggtggtc ccgcccacgg tcacgatcat ctattggaca gtaacataga ttggggtcaa
    19561 gatttattac cgctaaaacg gtggctcgac aaacatcctg agattaaact ttccgggatt
    19621 gcctattccg taccggctcc ctttgtccag cttgagaaaa ttgggcctgg cttcgatcgg
    19681 ttgccgccgg ggttgccacc gggttgtgct cccgagggcg cacacatcga acctggatgg
    19741 tacgttgtgt ttatcaggga aatgcgtgaa cagaatagtc catattggta tttcatgcga
    19801 ttgactccgg aatcgcggat tggttggaca gcttatgtgt accatttgac ggaagacaag
    19861 atccaggagg tgatgcgatc gctaaccaag gcaaaactat gaggtgcgtg agcatgcaaa
    19921 ccaattgcgg taacgagcct ccaagaaggg atggtgtttc cttgtcgcgg cgatcctttt
    19981 ggaaggtctt gtatgcaagt atagctttgg gtatggtact attgagtgcc gtgatagtcc
    20041 gtattccgta tttagccaca aggagcgtgt ggtatgatga agcctcgtcg tggcagatgt
    20101 cgcagttttc acttccggag ttgtggtacg cgtgccagtt gaatgttcat ccacccctgt
    20161 actaccttgt gctaaaggcg tggagtttcg tatggggcga ttcgcccgtc gctcttcgta
    20221 gtttatcgat cgcatgcagt gccttggcca ttgtgggcat cttcaccatc ggctggctgt
    20281 cgggaggagg taatggcgtc aaagacgcaa gagattttga caacggtaaa ctttatgtcg
    20341 ctatagtcgc cgcaaccctt atggctgtca acgcctttca ggtcaatgct ggcattgaag
    20401 ctcgtatgta tgcgctagga acggcattcg ctgtatggtc agtggtcgca acaatccgag
    20461 tctttcgaag caattcaacc ttgtggtgga ttgtacttgc atttctcctc ctgtcttgta
    20521 cgtacacaca tcattactgt ctgttggtag ctgcaagcga ggttgctgtc cttgctgtct
    20581 acgggttaat gcgctggctg agacattcgg atcactctgt tataaggcga cttggtggtc
    20641 tagtgacagg tgtggggttc ggattcctac cgggagtgct cttgcttcat attcaactcc
    20701 agcgggtgcg agagaactat tggaccgttc ctttgggtaa agacgtgatc gtgaaaacgt
    20761 tttcccagtt ctggcaacca attgaacctg caaatgccta tgaaagtggt ctatgtgttt
    20821 tcgttgtact attactgtgc acagccctca ctattcgtca tcgttcgatc gaaaaatgcc
    20881 ttgtatcgtg ttgtgcttgg ctacccattg tgttagctgg tatcgtaagt agcctggtca
    20941 ccccaatatg ggaggggcgt ttctttcgtt tcaatgagcc gtttttcctg gtagtgattc
    21001 cgctaggact ccagcagatt tcgcaacgac aaactttacg gtggggtttt gccgccattg
    21061 ttgttgcggt cgtatcctac ggaacatggg ttttctggaa tgcgcggcag gttgaacagc
    21121 gaggggggat gcgtgccgcg acggaattcg tgttgcagaa cagtaggccg tgcgagttgg
    21181 tgctcgctac cagcaatatt cactattttc cagctaagta ttaccttcgt ggacagcgcg
    21241 agataaagct tcttcgggat gccgtgcgtg agttctgggg acaccatctt attcggcccc
    21301 gagatctaat aggactggcc caactggagc gatttaggga acgcggcatt tggcttctgg
    21361 gacactcttc gcgtccccga gagattgctt ggactgacga tggccaggat catcgtgaga
    21421 tatttcatta tgactttggc gtgccccagt ggacagttta tgtgatccat attcaggggc
    21481 gggtcgcgtc ctccgaaaat aaacagggga ataagtaatg tgtttgcacg gtgtgcctga
    21541 tagtgtgggc aattgcgcat cgaagctaac ggacgactca tgcacacgaa actgggtgag
    21601 gtagaagcga tgcggtggtc gatatgctgg tgccttgtgg tagcgatctc tttgagtatg
    21661 ctcgtatcag cttgccagca cggcgggctg tcggactcag accgtcttaa acaggcagcg
    21721 caacaagtcc agttgggata cacagatcgc attgacatgg ggcaaaaaag caatattgga
    21781 gatcaggacc tggagttgct tgccgccgtt gagggattac acttccttcg cgttgatgga
    21841 agccgcatca cggatgaagg gctggcttac cttgtctcac agaaggaact aatagatctt
    21901 tatgcgtcca acacccgatt gtcggatgaa gggctaaaaa tactgagtgc gtgttcgtca
    21961 ttggaaaccc tagtcattga caagacgcaa gtgacggatg ccgggttagc ctatcttgca
    22021 gaacttccca acctcaggag gctgtcactt tggaaatgct ttatcacgga tgacgggtgc
    22081 cgacacctag caaagctgcc tcagctccaa gttttaagtt tggatgaaac cttggtaaca
    22141 gatgccggta tgaagtattt gacaggactg aagaatttga ggcgtatttc cttatggaaa
    22201 acacgtgtgt cagaccaagg agtcgcaatc cttcgtagcg cgcttcccga tttggagatc
    22261 aaccgatgag catgaatcgg gtgagacttg atcgcagtat cggtgactgt agggggttca
    22321 cccttattga gatactcgtg gtgatcacga taattggtgt gttgacgatg cttcttctcg
    22381 cggcggtgca ggcctcccgc gaggcagccc ggcgcgccca atgtcaaaat aacctcaaac
    22441 agttggccct cgcaatgatc aatcatgaga gtgcgatgag attttatccg tcaggaggat
    22501 ggggctatcg atgggtgggc gaccccaacc gcggtagcgg ccgacggcag ccagggggtt
    22561 gggctttcgt cattctcccg tatttggagc gccgagacct gtttgagcag gcattagata
    22621 tccatgatcc cagtgaacta cgctggtgcc ttgcgcaggt taacgccgtt gttgtgccga
    22681 cattttactg cccttcccgg cgggcagccg aactgtatcc cattttacaa agtcccgccc
    22741 actatccgta caatctcgac ttacttgaga atgccgtaaa aatagattac gcggcgaatg
    22801 caggtgattt cttgctcgat gtcggagctg gtccagtgtc gattgcccag ggtgaccatc
    22861 cagcttatcc ctggcctgac tatccggcta ccggagtatg cttcttgcgg agcgaaatct
    22921 cgatagacca tgttttggat gggactagca atacctattt ggtgggcgaa aagaatattc
    22981 cgcgattgca ttatgacacg ggggattggc gaggggatga ccagtctgcg tacgtgggag
    23041 atgattatga tacgatacgt tgggcgaacg cttctctgct cccactcaat gatgtcgaag
    23101 ctgaaaacga tgacgctcga tttggggggc cacatccggg cgtggtccaa atggcctttt
    23161 gtgatggctc tgtgcggccc attgacctca gcattgatgc cctagttcat caacaacaag
    23221 ccaaccgcaa ggacggacaa gtgaataaca cagtcagatc taatggcaac tgacagtctt
    23281 tcttttccaa aattcgtttt agaaacctct gtacacctgc cctatcccca gctctctgcc
    23341 tctttaacaa ataactcgcg tagtgccgta agtgcttttc tgaacgttat tggtattatt
    23401 tcgattatcg ttcgagcaat aatgaataag ctgcagggat gagacttgtt tcgactcgac
    23461 caagcaccac tcgggaaact tctcctgaca acttccccga tgcccacggg gacccagtgc
    23521 cccataacat acgtgaccca tggcatgtta gaatcggccc ggttacttgt cgagattggc
    23581 aatgctgatc cgttccgctc gcaaggcggc ggtcatccaa gccaccaggg tcttgacgcg
    23641 actctttcgc acatttgtcc aaatcatgag cgtccacgct gaaataccaa gcagtttcat
    23701 ggcggctccc tccgcatgtg ttgctcgtgg gttgagtttc gctcaacggt gcagtcaccg
    23761 ttgatcccgt aaatctatac gagatatgca gtggcgaggg agcccaattt tatagggtcc
    23821 agaaactggg aatagggcag gtcccatctc gcaaagaccg attttcagaa agagcctaca
    23881 taacggcacc ttggaggctt ttcgacatgc acggttttcg gaatgctgtg gagtcgttat
    23941 ggcgcgatct ggtcgattcg cattttgacg aagaacactt cattagcggt gtaatgcgtg
    24001 attgcggaca tgatgctaca ggcgtttcca agaccgattt tcaggaaatg ctgaatacag
    24061 gtagttgcgc agccatgcaa gaagtgggat ggtgctgtaa tgaggtgttg agctactatt
    24121 tagcaaacca tcttggcagc gcggagttcg ttcgagcgtt atttgttgca ttatgtgccg
    24181 tgagggccat ccaagccggt tttgcccatt gggatttcat atttgacaaa atcctaatgc
    24241 tgctggtgca aaacatggac aaaacagatt atcattttcg catggaattt ctcaggaata
    24301 ttctctgcga aaactcggaa ggaacgagaa gttgacaatt tgggatggct gggtcgcagt
    24361 gcaggcttgc gcaggacgac tgatgggagg ttgcgccagg gggattcaaa gatttacggc
    24421 cgcgtgggac atcgccgccg agttacgcgc cgagccgaag gggatataag attgaaaaaa
    24481 cgtattacct tgcctttgaa ggctggcgta tgattgtgcc gctcgaacac ggattcttgc
    24541 ggaagaaggt gcctggagta gcccgactcc tgcctgttct ctataaccat tggtacaagt
    24601 ctgaatgttt gaaactactt cgccgtcgaa ccaaggacga ggttgaacat gcttggcgag
    24661 cggcggtagt ccctaaccgc gtggccgggg tcgtgacgcg caccgtgagg gatgagtatt
    24721 gttggctgcc tgaggcgctt ttcgtgcccg ccgatgagtg ttttgtggtt ttctgctggt
    24781 ggcgactggc gttaagtgac ggtttggagg cggaggtatg tatagtcgag attgagcgag
    24841 agctggaagt tggaatcgcg gaggaggtga ttaggctgtt acccacgtta aaacttggcg
    24901 atttctgcag gaggatcagt aacacgcttg tgggccgtta aggcgggact tccaggattt
    24961 cgctaccaat ttattgggaa aattgtgctt ttgccgatgg ccatccacgg gaaccgcacc
    25021 ctgcggttca tcgacgccaa ccagaacggc cagctcgacc aaggcgacac cgacatcacc
    25081 cagtacactt gggaccaccg caaccggctg gtcaaggtgg agcatcgggc cagttacgcc
    25141 gcggcggtgg accgggtgat cgagaacgcc tacgactacg ccaaccgctg ggtccgccgg
    25201 cgcctggata gcgacggcga cggccatacc gaccagcggc ggattttcgt ctacgacggg
    25261 aaccagatcg tgctcgactt ccggcggacc ggctccgggc aggttcaggc aggcgacctg
    25321 gagtggcggt atctgtgggg accggcggtg gaccagattt tggccgagga aaa
//
LOCUS       DAOSUT010000051        24910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1309_length_24910_cov_9.846429, whole genome shotgun sequence.
ACCESSION   DAOSUT010000051 DAOSUT010000000
VERSION     DAOSUT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24910
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_24910_cov_9.846429"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLD05_06495"
     CDS             <1..771
                     /locus_tag="PLD05_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77120.1"
                     /translation="AMNHPAKDVIEEIRWIAENGFDFVDLTLEPPAADPSVLDLPSVR
                     KALARYQLGVVIHTAYYLPYAVPFRSVRAACREEFSHALRAAHQIGAELVTIHFSRPP
                     RFFTDDQVVEWHRDIVDPLCAMAENWGIRIALEQIPFPNCDQMGIIQRLLNALPNLGF
                     HLDSGHAQLERKGDLWYEYLERFGARLCHVHLSENDGTSDQHLPLGSVPHVGLDWPDR
                     IARLKAIPYDGTITLEIFSAEPEYLLQSRNLLRRWWDS"
     gene            complement(798..2132)
                     /locus_tag="PLD05_06500"
     CDS             complement(798..2132)
                     /locus_tag="PLD05_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOP77121.1"
                     /translation="MRKILRPMTGVNRDDLPGGVLVVIAKVFQGFQVMAYRRDIPRVA
                     LLIETSRGYGRQFLKGVIRYGRLHGPWGFYITPGDLEQVLPRMRRWGGTGIIARVETS
                     QAARAIVAAKLPTILVGLSSLRLRRNKSLAQFSQVHSDSYNAGRLAAEHLLERGFRHF
                     AYVGLPGYLWSQRREQGFCDRISEAGFTVDVYPLASNRTGYSRNTWEKEHRRLADWLA
                     TLPHPVGLMACNDDRGREVLEACRTAGIRVPEEMAVVGVDNDELFCELSDPPLSSVAL
                     NAEAAGYRAAALLDRMMLGEKIAPQEIVAEALHVVTRRSSEIVAMEDPDLTAALQFIH
                     DHVTEPITVADVVSAVHVSRRALELRFRRQLGRTIHEELERLRLQRAERLLIETELPI
                     PYVAEAVGYNSPNYFARIFQQKRLMSPTEFRRQKRGELLRQDLRVWKTMIAE"
     gene            2307..3014
                     /gene="araD"
                     /locus_tag="PLD05_06505"
     CDS             2307..3014
                     /gene="araD"
                     /locus_tag="PLD05_06505"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOP77122.1"
                     /translation="MWERLKAEVAEANRRLSRERLVTLTWGNVSAISEDRKAVAIKPS
                     GVPYEELTSDDIVVLGLDGTILEGKRRPSTDTPTHLAIYRGFEHVRGICHTHSRFATV
                     FSQWRQEIPCFGTTHADYFCGPVPVTRVLSPTEVEEDYELNTGKIIIERFRVGGLDPL
                     VIPGVLVAGHGPFTFGRSAHEAVDVAVALEEVAALAWHLLLLESQPSQIEGYILQKHY
                     QRKHGPKAYYGQDDHRI"
     gene            3051..4544
                     /gene="araA"
                     /locus_tag="PLD05_06510"
     CDS             3051..4544
                     /gene="araA"
                     /locus_tag="PLD05_06510"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOP77123.1"
                     /translation="MINLESKEVWFVTGSQHLYGPEALQKVAENAQKVVQGLNSDPVV
                     PVKILAKTVAATPDAIHRVCLEANTSPECIGLITWMHTFSPAKMWIAGLRDLSKPFLH
                     LHTQMEQDLPWATIDMDYMNLHQSAHGDREFGFLCSRMRLRRKVVVGHWKDPVVRQQI
                     GVWTRVAAAWDDLRSLRIARFGDNMREVAVTEGDKVEGQIRLGFSVNGYGLGDLVEKI
                     QQVSDFEVERLVREYDDLYDVAPELRADGSRRQDLFEAARIELGLRAFLVEGNFGAFT
                     DTFENLHGLQQLPGLAVQRLMAEGYGFGAEGDWKTAALVRALKVMSAGLRGGTSFMED
                     YTYDLKPGNMKVLGAHMLEVCPSIAAGRPSLEIHPLSIGGKRDPVRLVFDAAPGPAVN
                     VALVDMGNRFRILVNDVEVVAPEEELPRLPVARAVWIPRPDLKTSAAAWILAGGPHHT
                     AFSQAITAEYIEDLAEIAGVEFLHIGANTQLEQFKKELRWNEAAFSL"
     gene            4614..6557
                     /locus_tag="PLD05_06515"
     CDS             4614..6557
                     /locus_tag="PLD05_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOP77124.1"
                     /translation="MTPVDWAVIAGYFVILFGLAWWVILRGKDTADDYFLAGRNLGWF
                     IVGASIFASNIGSEHLVGLAGSGCTDGVAMAHYELHAWCLLILAWVFVPFYLRSKVFT
                     MPEFLERRFSPGARWLLSLISLVAYVLTKIAVGIFAGGIVFQTLLPEVHLNVGPIEMN
                     SFWVGSVLVILLTGLYTVLGGMRAVAYTEALQTFILVAGSALVTIYGLIALGGWGTLR
                     EVCGGEMFNLWKPLAPEGWTGTWKPVIDQEAKVMAWYFNTNYPWLGMLICAPISGLWY
                     WCTDQYIVQRCLGAPSEREARRGAIWAAVLKLLPVFIFIIPGMICLALSISGKAEALR
                     DEFFQAELEKALTQGAAEVKRIAVGRVIVEGGQQKVIAGQQVVTNGKIAVVGGTVEPV
                     EGGLAVTKGAVYVNAHATQAAFPLMVKYVLPPGFRGVVVAGLLAALMSSLAGVFNASS
                     TLFTMDFYSKLRPSASQHELVWTGRVATTTMVLIGLAWIPVIQGARGLYDYLQSVQGY
                     LAPPIFTVFFLGVFWKRLNAKGCLWALAIGFLLGVFRLAVDTPVKLFGVQYPEGSFLW
                     IVNNIFFQYFAVLIFVVSVTVMVVASYLSSPPDEQKIQGLTFGTVTEEQRRQSRATWN
                     WRDVAASGLVLAAIIAAYLYFRG"
     gene            6744..9143
                     /locus_tag="PLD05_06520"
     CDS             6744..9143
                     /locus_tag="PLD05_06520"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOP77125.1"
                     /translation="MNVMYRLSFVARVCVFLWVANTGFLACGEEQTITLTVHPKEVLN
                     TIDVNVYGHFLEHIYHSVNGGLWGELVWNRSFEHHEGGRWQVIDQVVVQKGLATNQRL
                     VFGDPEWEDYEFTLEAQKLGGEEGFLILFRVRDETNFYWCNLGGWGNRWHQLERGRSG
                     EGRWYPVGPRVAGQIDSNRWYRIRIRCEGDHFRVWLDDQQIIESRDPQGHRKGAVGIG
                     TWATQAGFRNLRVTDLAGKELFAGIPEVGSLQQVAKHWRVFGQGRAFLDDNQPLNSDW
                     SQRLESQGGETGLEQQPFALKANEWYVGSLWIRGNTEQNPVIRLMNGPDIIAMQSLPR
                     PGEEWQEVPIRLRSTRDVDSATLQIAFRGPGRLWIDQVSLMPESWLKAGGYRPDLLQA
                     VADLQPPVIRWPGGCFASAYRWKDGIGPQAKRRSYPREIWDDIDVNSFGTDEFIEMCR
                     RVGAEPLIVVNIGTPQWNPGANPDEFLQDILDWIEYCNGPADSKWGRVRAENGHPQPY
                     GVKYWEIDNETWGMTAERYAAAVRRIAPLMRAKDPTIKLAACGSGGMGESAHRWNRVI
                     IEQCAELIDYLSIHHYENPDRFADGPRNYEEFFRATGELIARSKNPNLKLYVSEWNAQ
                     STDWRTGLYCGGILNAFERTGNIVGMAAPALFLRHVSATAWDNAFINFDHRKWFPAPN
                     YVVMQLWRRFYLPNRIRIDGDTGRLNAVATASEDGRRVVVKIVNPTEVTHTLRVQSAG
                     GRIAAAQAVQVAADRLMARNTLDNPSLIRPQTLQTQLTSEGATVVVPRYGCVIVEMEL
                     K"
     gene            complement(9403..9795)
                     /locus_tag="PLD05_06525"
     CDS             complement(9403..9795)
                     /locus_tag="PLD05_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77126.1"
                     /translation="MRKELTQRLLARVGVLEDKGCPSRRNDSCACVLMENAPSIVAQD
                     EDEDWDDDEDWDDEEDEEEDWDDEDEDWDEDEDEDWDDEDEDEDWEDEDLDEDDEDWE
                     DEDLDEDEDWDDEEDEEDWDDEDLEEDE"
     gene            complement(9995..10744)
                     /gene="scpB"
                     /locus_tag="PLD05_06530"
     CDS             complement(9995..10744)
                     /gene="scpB"
                     /locus_tag="PLD05_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOP77127.1"
                     /translation="MNWWHNPWTTCYGGWPGAMAQGNRFWRLHPPTTAATKLLWSLSP
                     EEDVSRQPVADAFSAEFSDCDHQLARVEAVLFLARQPISARKIAQIAGLDSGSQVRAL
                     LRRLNKLYDEKRMAFRAIEVAGGFQLRTRACFEPWIARLVKDPHQTRLSPPALETLTV
                     IAYRQPILRAEIESIRGVQCGEVIRQLLERGLIRVVGRSEELGRPYLYGTTARFLEMF
                     GLRGLDDLPHPEYRIPSVALRRPVAEASNGG"
     gene            complement(10911..11813)
                     /locus_tag="PLD05_06535"
     CDS             complement(10911..11813)
                     /locus_tag="PLD05_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77128.1"
                     /translation="MTDFDNETTVEMAQVCPKMSFSEVLRALILFGLLFGMVALASIP
                     QGTLPALVLLHTDEKPSLCPAKNPDASPTVNLAANEQTPPGRFVMGSQTQAQAIQATV
                     ALAPVTQSALSHLENHPRLDDNSGPATAAPIRETLEDKRSFGMCLHHEATPSPSIQSV
                     QPLDGVTSSGFTSWDYQATSEAGGGQRAYTMVSPSATLESFGSVETMSPGPSVSLPPY
                     RGEEPGLRDWAFRFEKLGAVRYRLETWGNRGELYRFWCEMPVGQSSGTVRFFEAVASR
                     PEEAMENVLRQVEQWLQDASPVPR"
     gene            12051..13373
                     /locus_tag="PLD05_06540"
     CDS             12051..13373
                     /locus_tag="PLD05_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77129.1"
                     /translation="MYVLSAGRCLLVCLSLLGTACQFFGQAENHNSVDQDQAAGAGLE
                     AQVEELLSRLDAPQRAVRDQAERDLMALGRQAIPFLQPKRMLSPEAALRLARVRTTLI
                     EQIVATDALPTRVTCKVTDLPLSEVVTKIAHQTGNAVTVSDEVAKLPVTWDCQEKTFW
                     EALDELAALVDADVSPAETGGLVILPQKNRAVQSRVAVAYCGVFRVRLRDVIKIERQH
                     NTSQVSLVRIEIAWEPRLQPAVIWWRGIHIDREQPESAAIEDKTLARREVPVSPQRCF
                     IILTLPVTLPSDLEQKPAVLRGSFEPVLPIGATDVVFDLTDLQKGPRSVILGRSEITA
                     ELQRRSANTCEVSLRVRYEEACGAFASHRGWFYSWPVYLDTDGKKALPRGVQLLRMTE
                     DGVTLLYQFGNAEVTGTRMVCRVPLAIVKPVVEFQLALSLEERNRSKD"
     gene            complement(13480..14064)
                     /locus_tag="PLD05_06545"
     CDS             complement(13480..14064)
                     /locus_tag="PLD05_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77130.1"
                     /translation="MRDHQSEGPGKPGKRNGRRSPGTGRSRKKTDSQRRTGQSQGISV
                     RRIQDDVYELVYPRKARLLRDDIEEVYDMIAHEEWDLAVDELLWLLRECRELLEAHQL
                     LGRIALFQNKLDLARAHLGHAYELGLNATGKDFRGRLPFTQAANRPLLQATRDLVECL
                     IRLNERDLATSVAQQLLQWDPSDPLHVRDLCPPS"
     gene            14403..14900
                     /gene="tsaA"
                     /locus_tag="PLD05_06550"
     CDS             14403..14900
                     /gene="tsaA"
                     /locus_tag="PLD05_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOP77131.1"
                     /translation="MKSIVVLGIVLTSSMTAICLAESPTSFTVYPIGRVEKTDGRCRI
                     VLDEKYKPGLLGLEQWSHVQVLWWFDKNDTPQKRAIVQVHPRGDKNNPLTGVFACRAP
                     VRPNLIGLTLCKILRVQDNVVEIDDIDAFDGTPVLDLKPYAKGEDTADEVRVPEWAKR
                     APPRN"
     gene            15093..>15445
                     /locus_tag="PLD05_06555"
     CDS             15093..>15445
                     /locus_tag="PLD05_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77132.1"
                     /translation="MRVIFALLIVVALTCPVCAEVVVYPAPSGEELSADYRVSVAGQD
                     VAVYTARTLDPPFAGKQWDYGGPYSFASFDTDEPVEVRITSPRDLRNTVIRPSYPDVT
                     LHVVNEHEVVVRLPGP"
     assembly_gap    15446..15545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15546..16220
                     /locus_tag="PLD05_06560"
     CDS             <15546..16220
                     /locus_tag="PLD05_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOP77133.1"
                     /translation="HWTIVPVHSQRVTIRNVKICGGRVQNDDGINPCNSQDVLITDCF
                     IRSDDDCVALKGLDFTEGNNNVERIRVENCILWCDRARIFLLGHESRAPFMRDIVLTD
                     LEIIHFSMTPFLLEPGEDMRLENVLVLGVRIHGEGQQELIRLRPVVNQYMRKKTPGFI
                     KNVTFKDVTVYGAPGGYRVQIHGADAEHNVRDVTFDNVVILDEKLTGESSRLETGPHV
                     EGVTFK"
     gene            complement(16361..17794)
                     /locus_tag="PLD05_06565"
     CDS             complement(16361..17794)
                     /locus_tag="PLD05_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77134.1"
                     /translation="MNGRHFFWTVLVATSLISQLALAQELYVGLSVVDITPSEPVALD
                     GQRHVRIAKEAATPITATVLAMESRQGEQVLDQAVIVSCDLVAIREGVCQMVREKVVR
                     LKNDLDPLKIFLCATHTHTAPVTVEGKYTLPETGVMRPSAYTDWMTTQIAQAIGQAWE
                     KRQPAQVGWGQGQAVVAQNRRAVYADGSAKMYGATNVPEFRGMESQEDHNLEVLFFWN
                     NEGKLIATAINIACPAQEVEGLSVIHADFWHPVRELLRSRYGSDLAILGLTGAGGDVT
                     PHLMYGKAADERMRKLRNLSRLDEIARRIVRGWEEAYEVAKLDMRKDVVLKHVVQTIE
                     LPYRRVTEAEAEEARKLAAEYADKPSERWNYLWHQRVVDLYEKQQSGEIGALPMELHV
                     IRLGDIAIATNPFELYTDYGIQIKARSPAVQTFVVQLAVGSGGYLPTARAVQGGGYGA
                     VIQSGRIGPEGGQVLVDRTVEAISQLW"
     gene            18090..19265
                     /locus_tag="PLD05_06570"
     CDS             18090..19265
                     /locus_tag="PLD05_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP77135.1"
                     /translation="MKSTLLCAVVIFVVFRMLPIGLCGEIRFRHHFVDRDLPGNSWGQ
                     TAVADLDRDGRLDYITGKSRGQIVWYRQISLTQWERHILGEDSPSDVGGAVLDVDGDG
                     WMDFVTGGAWYRNPQRPTEGPFERIVFDKELQGVHDVVVADVNGDGRLDVLTMSDRNN
                     IRWYQIPENPRELWVRTDIGPSVHAGLAVGDLDGDGDLDVIRSNLWFENVDGKGKTWN
                     VHENIPFGNPSPPYALATRCIAVDIDSDGDLDLVMTENEIRGGRIGWLENLGYGKAWN
                     LHLLPQGDASIRGAYHSLIVADFDLDGDWDVFSCEMEGIMGEKLPRWFVWENVDGKGQ
                     NWVEHVVLDAGLGGHEAVAGDFDGDGDLDIISKLWRPRKDNANGGKNHVDLLENLRR"
     gene            complement(19427..20989)
                     /locus_tag="PLD05_06575"
     CDS             complement(19427..20989)
                     /locus_tag="PLD05_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP77136.1"
                     /translation="MFDCLWSVALLGAVLLTSSYTAFGVEDAQAPAGRLPNIVLILAD
                     DLGYGDVGCYNPESKVPTPHLDKLAAEGMRFTDAHSAATVCTPSRYSLLTGRMCFRTG
                     MRGVFTGAGGPCLIEEGRLTLPQLLRNKGYATACMGKWHVGLTFFDKEGRPIHQDGLE
                     AVKRIDYTRPIPDGPIHRGFDYFFGTACCPGTDWLYAYIEGDRIPVPPTKPLDRSGLP
                     QHPYSEDNRPGLIAPGYNLEELDLVFLEKSRQWLERHVREHPGQPFFLYHATSAVHLP
                     SFPAAQFQGATKAGPHGDFIFELDYVVGQLMQTLERLGVADNTLVIFTSDNGPEVTTV
                     LHMRADYGHDGARPWRGLKRDNWEGGHRVPLIVRWPGRVKPGSICELPICHTDIMATC
                     AQIVGAKLPDNAAEDSNSFLHALLGRSAPEDADRIVLHQTISLALGIRRGAWKYLDHK
                     GSGGNRYDRGALAALAIEDTAPDAPGQLYNLAEDPGERINLAGQKADLAAELKRLLEH
                     FKATGRSAPFHP"
     gene            21780..23462
                     /locus_tag="PLD05_06580"
     CDS             21780..23462
                     /locus_tag="PLD05_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP77137.1"
                     /translation="MRFLSGLFAVLICLHTVSLAIEPANPNLCQEGKELLAFFESIYG
                     KQVLAGYNVYPHTPDDYQQTARQAAIWGRDIRWLGDVEEVVSHVRAHGYFLTLHWHWF
                     FDEDSAWTRERKKPVDLAKVITPGTQEHETLMKELAATADVLERLQKEGIVVLWRPLH
                     EIDGGWFWWTNLNHPENTAELWRIMFRYFTDERKLNNLIWVYSAGVGDLKKKPAEWRR
                     RFYPGDAYVDISGIDIYGVDPKNDETPYWDYYKLMEEVSPGKMLALCECDAIPNPDKM
                     AAGKTPKWLYALPWWGCPSPRRSVEWARFTMGHELVLTLDEIPTMGRKDLVPQIGIVE
                     PNDDGSAWFPKSEVTMSGYAVSRGAAVARVEFLANDEVIGTLEKPPAHFSWSWVSPRA
                     GTFDLTARAVDTLGRTNTSNRIHLAVGVTNAAKNKPVRVSSGEDPQAAVDGSYYTSWR
                     AAKDAEEATCEIDLGQAMAVSQVNLIWGWKIHPTRFVIEVGSGESWDQVRDWKTVATM
                     ENLPWVTWKATHRVRFEPVTARFVRIRMFQRANRQTWSGYDLAEIEIPVPVK"
     gene            complement(23629..>24910)
                     /locus_tag="PLD05_06585"
     CDS             complement(23629..>24910)
                     /locus_tag="PLD05_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77138.1"
                     /translation="EAQFWGFNRYADWFDMEDCSDPFAQTRLYQLAHAMWDRKKANFA
                     IAQKLGFACDLVITPNHVYVDQCKDQWKAVQGGRIFGQLICPSHAEARKVILRNYENL
                     FADLARSGVRLSALCSAPYDFGGCACERCKPWIITFAQLSKEIYDIGKKYHPNLEMHM
                     IGWWWEPEEHRLFADWVNTHCEGWIQRMYLHIPYGQTTVADVPLPTGCEISAFVHIGY
                     AEEASPRDTYGHLGPVVAAERLQKTVQELAARGCTGVMAYSEGVFDDVNKALLAGLSS
                     GQFSDSHAVLTSYAERYFHATGENAQKWAQWLRAWGRPFSVDPVVAAQSLQELPRPEP
                     RSACGSNWRWEQWRLKCELFRHHREIGTGSEWTPEWLAAVDRFWAVQEELHRKVWGLP
                     PLRHIFARRFTPLPWYASWAKYQAQQAEAMGKEQ"
BASE COUNT         5328 a         6546 c         7185 g         5751 t
ORIGIN      
        1 gccatgaacc atccggcgaa agatgttatc gaagagattc gctggatcgc agaaaacgga
       61 tttgactttg tcgatctgac gttggaacca ccggccgcgg acccatcggt tctcgacttg
      121 ccgagtgtac ggaaggcctt ggcacgttat caattaggcg tggtaatcca cacggcctat
      181 tacttgccgt atgccgttcc gtttcggtct gttcgtgcgg cgtgccgcga ggagtttagc
      241 catgccctgc gggcggcgca tcaaattggg gctgagcttg tgacgatcca tttttctcga
      301 ccaccccgct tttttacgga cgaccaagtg gtggagtggc atcgcgacat cgttgatccc
      361 ctttgcgcga tggccgaaaa ttggggcatc cggatcgcct tggagcagat tccttttccc
      421 aattgtgacc agatgggtat tattcaaagg ctcttgaatg ccttgccaaa cctcggtttt
      481 catttggaca gcggccatgc acaactagaa agaaagggtg acctgtggta cgagtacctt
      541 gagcgatttg gtgcacggct ctgtcatgtt cacctctccg aaaacgatgg gacatcagac
      601 cagcatttac cgttggggtc cgtgccgcat gtggggctgg attggcctga tcgaatcgcc
      661 cgattgaagg cgatccccta tgacggcacg attaccctgg aaatcttctc tgccgaacca
      721 gagtatcttc ttcaaagccg taatttgctt cgtcgttggt gggattcttg aaaagaaaag
      781 attattagga gagtgtctca ttcggcgatc atcgttttcc agacgcgaag gtcttgtctg
      841 aggagttctc ctcttttttg ccggcgaaat tctgtaggac tcatcaagcg tttttgttga
      901 aagattcggg cgaaatagtt gggtgaattg tatccaacgg cttcagcaac gtatgggatg
      961 ggcagctccg tctcgatgag gagtcgctct gcccgttgga gacgtagtcg ttcgagttcc
     1021 tcgtggatgg ttcggcccaa ctgtcgacga aaacgtaact caagggcacg ccgggagacg
     1081 tgaacggccg acacaacgtc ggccaccgta attggttctg tcacgtggtc atgaatgaac
     1141 tgcagggccg ccgtaagatc agggtcttcc atggccacaa tctcactcga ccttcgtgta
     1201 acgacgtgaa gggcctccgc gacgatttct tggggggcaa tcttctctcc tagcatcatc
     1261 cgatccagaa gtgccgccgc tcgatacccg gcggcttccg cgttaagtgc cacactggat
     1321 aaggggggat cactcagctc acaaaagagc tcgtcgttgt ccacccccac aacagccatt
     1381 tcttccggga ctcggatgcc cgcagtacgg cacgcttcca agacctctcg tccacgatca
     1441 tcattgcatg ccattaaacc tacgggatgg ggcagcgtgg cgagccaatc tgccaaccga
     1501 cgatgttcct tttcccacgt gtttcgggag tatccggttc ggttcgacgc caaaggatac
     1561 acgtcaaccg tgaaccccgc ttcggaaatg cggtcgcaga agccctgctc acgcctttgg
     1621 gaccacaaat acccgggcag tcctacatag gcaaagtgac ggaagcctcg ctccaaaaga
     1681 tgttcggcag ccagtcgccc ggcattgtac gagtccgagt gaacctgact gaattgggca
     1741 agcgatttgt ttcggcgaag acggagactt gaaaggccaa ccaaaatggt gggcaatttc
     1801 gctgccacaa tggcgcgtgc tgcttgggaa gtttccaccc tggcaataat gccggttcca
     1861 ccccaacgtc gcatccgcgg aaggacctgt tccagatctc ccggggttat gtaaaagccc
     1921 catgggccat ggagccgccc gtagcgaata acgcctttca agaactggcg accgtaccca
     1981 cgggacgtct cgatcaacaa ggcgactcgg gggatgtcac gtctgtacgc catgacctga
     2041 aaaccctgga agactttggc gattacaacg agtacaccgc cgggaaggtc atctcgattg
     2101 acacctgtca tcgggcgaag gattttgcgc aattgtatcc tcattttgcg caactgtcgc
     2161 ttgccaagcc acggttgtct ggctattctg tcgttttatg cctgtcgcat ctttttgtga
     2221 tcgcggcaag gccgagcccg tcgcactggg ggatcggtga tggggcccgg aaactcctta
     2281 gtcgccgtca taaggacagt ggtcgcatgt gggaaagatt gaaagcggaa gtggccgagg
     2341 ctaaccgacg gctttctcgg gagcgtttgg taacgcttac ttggggaaat gtcagtgcca
     2401 tcagtgagga tcgaaaggcc gttgccatca aaccaagcgg ggtgccttat gaggagctca
     2461 cttccgatga catcgttgta cttggactgg atggcacaat cttggagggg aagcgtcgtc
     2521 cttcgaccga tacgccgaca catcttgcga tttaccgggg ctttgagcac gtgcggggta
     2581 tttgccatac ccacagtcgg tttgccaccg tgttttcgca atggcggcaa gagatccctt
     2641 gttttgggac aactcatgcc gactattttt gcggcccggt ccccgtaacc cgagtactaa
     2701 gcccgacaga agtggaagag gattatgagc taaacacggg caagatcatt atcgagcggt
     2761 ttcgcgtcgg ggggcttgac ccgctcgtca taccgggcgt tttggtggca ggacatggcc
     2821 cgttcacatt tggccgatcg gctcacgaag ccgtggatgt tgccgtggcc ttggaagaag
     2881 tggcagcttt agcctggcat ttgttgcttt tggaatcgca acccagtcaa attgagggat
     2941 atattctgca aaaacactat caacggaagc atggtcccaa ggcctactat ggccaggatg
     3001 accatcgtat ttaaaacgcg gagctgagca gagacgtagg gttggaaggc atgatcaacc
     3061 tggaaagtaa agaggtttgg tttgtcacgg gaagtcagca tctttatggc cctgaagcac
     3121 ttcagaaggt cgccgaaaac gcgcagaagg tcgttcaggg gcttaacagc gaccccgtcg
     3181 ttccggtgaa gattttggcg aagacggttg ccgcaacgcc cgatgccatc catcgcgtct
     3241 gtctcgaggc taatacctcg ccggaatgca tcggtctgat cacatggatg cacacctttt
     3301 cgcccgccaa gatgtggatt gcaggcctgc gggacttgag caaacccttc ttgcatttgc
     3361 acacgcaaat ggagcaggac ctgccctggg cgacaatcga catggattac atgaatctcc
     3421 atcagtcggc ccatggcgat cgtgaatttg gttttctatg tagccgcatg cggttgcgcc
     3481 gcaaggttgt ggtcggccat tggaaggatc ctgtcgttcg gcagcaaatc ggcgtatgga
     3541 cccgtgtcgc tgccgcgtgg gacgatttgc gatcattgcg gattgccaga tttggtgaca
     3601 acatgcggga agtcgctgtg accgaaggcg acaaggtgga gggccaaatt cgcctagggt
     3661 tttctgtgaa tggttatggg ctgggtgacc tggtcgaaaa gatccagcaa gtaagcgatt
     3721 ttgaggtgga gcgactggtg cgggaatatg acgacctgta cgatgtcgcc ccggaactgc
     3781 gtgcagatgg gtcgcgcagg caagaccttt tcgaggctgc ccgcattgaa ttgggactaa
     3841 gggccttttt ggtcgagggg aatttcggtg ccttcacgga tacatttgag aacctccatg
     3901 gcttgcagca actgccgggc cttgctgtcc aacggctaat ggccgagggt tacggtttcg
     3961 gtgccgaggg agactggaag accgcggctt tagtcagggc cttgaaggtc atgtcggcgg
     4021 gactacgggg tgggacgtcc ttcatggaag actatacata cgacctcaag cccggcaata
     4081 tgaaggtcct cggcgcacac atgttggaag tgtgtccgag tattgccgcg ggcagaccct
     4141 ccctcgaaat tcatccccta tcgattggcg gaaagcggga tccggttcgg ctcgtcttcg
     4201 acgccgcccc tggcccagcc gtgaatgtgg ctttggtcga tatggggaat cgttttcgca
     4261 tactcgtcaa cgatgtagag gtggtggcac cggaggaaga gctgccgcgg cttcctgtgg
     4321 ctagggccgt ctggattcct cgaccggatc tcaagacatc ggcggcggca tggatcctgg
     4381 ccggtggtcc gcatcacacg gctttcagtc aggccattac ggcggaatac attgaagacc
     4441 tggccgaaat cgccggggtc gaattcttgc acatcggtgc gaatacgcag ctcgaacagt
     4501 tcaagaaaga acttcggtgg aacgaagctg ctttctcgtt ataaggaata tcactggtgt
     4561 atttcaaaac gtacggtaac gtgaggatgc ctagggattg aagggttaac gcgatgacac
     4621 ctgttgattg ggcggtaatt gctgggtatt tcgtcattct ttttggatta gcttggtggg
     4681 tcattctgcg aggcaaggat acggccgacg attatttctt ggctggccgg aacttgggat
     4741 ggtttatcgt gggggcatcg atctttgctt cgaacattgg ttcggagcac ttggtagggc
     4801 tggcagggtc tggctgcacc gatggtgtgg cgatggccca ctatgagctt catgcgtggt
     4861 gtctcctcat cttggcctgg gtttttgtgc cgttttattt gcggtcaaaa gtcttcacga
     4921 tgccggaatt tttggaacgc cgtttcagcc cgggggcgcg atggttgtta tctttgattt
     4981 ctttggtggc ctacgtcttg accaagattg ccgttggtat tttcgctggt ggaattgtgt
     5041 ttcaaactct gttgcctgag gtacacctca atgtcggccc aattgaaatg aatagctttt
     5101 gggtcggttc ggtgctcgtc atccttctga cgggtttata tacagtgctc gggggtatgc
     5161 gagcggtcgc ttatacggag gcccttcaaa catttattct ggttgcgggt tcggcactgg
     5221 tcacaatcta cggcctgatc gccttaggag gctggggaac tctccgcgag gtctgcggcg
     5281 gcgaaatgtt taacctttgg aaaccgctgg ctccggaggg atggaccggc acctggaaac
     5341 cagtgattga tcaggaagcc aaggtcatgg cgtggtattt caacaccaac tacccatggc
     5401 tcgggatgct gatttgtgct ccgattagcg ggctttggta ttggtgcacc gatcagtata
     5461 ttgtgcaacg gtgcttgggg gcacccagtg aacgagaggc gagacgaggg gcgatctggg
     5521 ccgctgttct taagctgctt ccggtgttca tcttcatcat acccggaatg atttgtctcg
     5581 cattgtccat cagtgggaag gcagaagctc tcagagatga gttctttcaa gcggaattgg
     5641 aaaaggcact gacccaaggg gctgcagaag tcaaacgcat cgctgtaggt cgcgtcattg
     5701 tcgaaggcgg tcagcaaaag gtcatcgccg gtcagcaggt ggtgactaat gggaaaatcg
     5761 cggtcgttgg cggtaccgtg gaaccggtgg agggcggact ggcagtgaca aagggcgccg
     5821 tctatgtcaa cgcccacgcc actcaggccg cgtttccact catggtcaaa tacgttcttc
     5881 cgccgggttt tcgtggggtc gtggtcgccg gactgcttgc cgctctcatg agctcgctgg
     5941 ctggtgtctt caatgcctct tcgacgctat tcaccatgga cttttatagt aagttgcggc
     6001 catcggcgtc acaacatgaa cttgtgtgga caggtcgggt tgccacgacc actatggtgc
     6061 tgattggctt ggcgtggatt ccggtcatcc aaggggcacg gggattgtat gattacctgc
     6121 aaagcgtcca aggatactta gcgccgccta tttttaccgt atttttccta ggtgtattct
     6181 ggaagcgact taatgccaag ggatgtctct gggcgctggc aattgggttc ctcttagggg
     6241 tcttccggtt ggcggtggac acccccgtca aactttttgg tgtccaatat cccgagggtt
     6301 cgttcctctg gatagtcaac aatatcttct tccaatactt tgccgttctg atcttcgtgg
     6361 tcagtgttac ggtgatggtt gtagcaagtt atctgtctag tcccccggat gagcagaaga
     6421 tccagggact gaccttcggg acagtgaccg aggagcaacg gcgtcaatcg cgcgctacct
     6481 ggaattggcg tgatgttgcg gcgtccggcc tggtgcttgc agcaatcata gcggcctatc
     6541 tctacttccg aggttgagct ggaataggct tgatatgaga gacaccacac cgcccacaag
     6601 ggctgttggc acgcggcagg tgtcgcgata tttgaatggt cgatttttag gatcaatttt
     6661 tcgggaaggt acgctttcgg ggttgttcga ccgtgaggcg agcctaaaca aggttttcgc
     6721 accaaatcgg aggttagttt gtcatgaatg tgatgtaccg tttgtctttc gttgctcggg
     6781 tgtgtgtgtt cttgtgggtt gccaatacgg gatttttggc ctgcggcgaa gagcaaacta
     6841 tcacgttgac tgtccatccc aaagaggttc tcaacacgat tgatgtcaat gtgtatgggc
     6901 attttttgga acacatttat cattccgtga acggagggct gtggggcgag ttagtatgga
     6961 atcgcagttt cgagcaccat gaaggcggac ggtggcaggt gattgatcaa gtggttgttc
     7021 agaagggatt agccaccaac caaaggctgg ttttcggaga tcctgaatgg gaggactatg
     7081 aattcacact ggaggctcaa aagctcggcg gcgaggaggg tttcctgatc ctgttccggg
     7141 ttcgcgatga gacaaacttc tactggtgca atcttggtgg atggggaaac cgctggcatc
     7201 aattggaaag gggacgtagc ggagaaggcc gatggtaccc ggtggggccg cgggtcgctg
     7261 gtcaaatcga tagcaaccgg tggtaccgaa ttcggatccg ctgcgaaggc gatcactttc
     7321 gcgtgtggtt ggacgatcaa cagatcattg agtctcgcga tccccaaggc catcgcaagg
     7381 gtgctgtggg gatcggtact tgggccacgc aagccggttt ccgcaatttg cgggtgaccg
     7441 atcttgccgg gaaagaactg tttgcaggca ttcctgaggt agggtccctt cagcaagtgg
     7501 ccaagcactg gcgtgtgttc ggtcagggac gtgcgtttct cgatgacaac cagccactga
     7561 atagcgattg gagccaacgt ctcgaatcgc agggaggcga gactggactt gagcagcagc
     7621 ccttcgcttt gaaggcgaac gagtggtacg tggggtcact atggattcgg ggaaacacgg
     7681 agcagaatcc cgtgatccgg ctgatgaatg gccccgatat cattgccatg caatccctgc
     7741 cccggccggg cgaggaatgg caggaggttc caatccgtct tcgttccacg cgcgacgtgg
     7801 attcagccac gctgcagatc gcattccgtg gtcccggacg cctttggatc gaccaggtga
     7861 gcttgatgcc ggagtcgtgg ctgaaggcgg ggggctatcg ccccgatcta ttgcaggcgg
     7921 ttgccgatct tcagcccccg gtgatccgct ggccgggcgg ttgttttgcc tcggcctatc
     7981 gctggaaaga tggcattggt ccccaggcca agcgacgaag ttatccgcgc gaaatttggg
     8041 acgatattga tgtcaattcc tttggtacgg atgagttcat tgagatgtgt cggcgggttg
     8101 gggccgagcc gctgattgtt gtgaatatcg gcacgccgca atggaacccg ggagcgaatc
     8161 ccgacgaatt ccttcaagat atccttgact ggatcgagta ttgcaacggg ccggccgatt
     8221 caaagtgggg gcgggttcgc gccgaaaatg ggcatccgca gccctatggt gtcaagtact
     8281 gggagatcga taacgaaact tggggtatga ccgccgaacg atatgccgcg gcagtccgcc
     8341 ggattgcccc attgatgagg gccaaggacc caacgatcaa gctggcggct tgcggcagtg
     8401 gcggcatggg cgagagcgct caccgttgga atcgggtgat tattgagcag tgtgcagaat
     8461 tgatcgacta cttgagcatc catcattacg agaatccaga ccgctttgct gatgggccaa
     8521 ggaattacga ggagtttttc cgagccacgg gagaactaat agcacgatct aagaatccca
     8581 atctcaagct ctatgtttcc gagtggaacg cacaaagcac tgactggcgg acggggctgt
     8641 actgcggtgg gattttgaat gcattcgaac gaacaggcaa tatcgtcggc atggcggccc
     8701 ctgccctatt tctccgccac gtctcggcga ccgcttggga caatgccttt attaatttcg
     8761 atcaccgcaa gtggttcccc gctcccaatt acgtcgttat gcaactgtgg cggcgtttct
     8821 atctccccaa tcggattcgc attgatggag atacgggccg cctcaatgcg gtggcgaccg
     8881 cctcagagga tggaaggcga gtggtcgtga aaattgttaa cccaaccgag gttacccaca
     8941 cactccgcgt ccaaagtgct ggagggagga ttgcggctgc ccaagcagtt caggtcgcgg
     9001 cagaccgttt aatggctcgt aacaccttag ataatccctc gttgattcgc cctcagacac
     9061 tacaaacaca gttgaccagc gaaggggcca cagtggttgt cccgaggtat gggtgcgtga
     9121 tagtcgagat ggaactgaaa tgacgagctc cttcaggtta ctaacctgag gaggcatgcc
     9181 gaccgtttcg gttaggagcg tcggcctcaa ttgctcgatc gactcctgaa aaacgagaag
     9241 gccgcccgac agtagctttt gccaacaaaa gatggacagt ggttacacct tctcgtaacc
     9301 actgtccaca attgtgaaac cgtctgtcgg ccgttcaaac tctataacgc ggtctgactc
     9361 gcaagacatc cggcgattgg acgcggcgag gacttttagt gcttactcgt cctcctcaag
     9421 atcttcatcg tcccaatcct cttcgtcctc ttcgtcgtcc caatcctcgt cttcgtcgag
     9481 gtcttcgtcc tcccaatctt cgtcgtcctc gtctaaatcc tcatcctccc aatcttcatc
     9541 ttcgtcttcg tcgtcccaat cctcgtcttc gtcctcgtcc cagtcttcgt cttcgtcgtc
     9601 ccagtcttct tcctcatcct cttcgtcgtc ccaatcttca tcgtcgtccc aatcctcgtc
     9661 ttcatcttgg gcgacgatcg atggggcatt ctccatcaga acgcaggcac aggaatcatt
     9721 cctgcgactt gggcagcctt tgtcctccaa gacacccact cgagccaata agcgttgagt
     9781 caattctttt ctcacggtga cacccctgac aaaaaactaa ggccaaacag ttgattccga
     9841 aacgcggaaa gcctggcctt ggtgacctct ccgcggttct tgtctccgca atattcaaat
     9901 aggttttcga ctacccacgc acttatcgtc aatccagcca aacgtgccga aacgtcaagt
     9961 atatcgtcct tttggcctaa cgataagagg ttgttcatcc accgtttgat gcttcggcca
    10021 caggacgcct caacgcaacc gatggaatac gatattcagg atgtggcaaa tcgtccagtc
    10081 ccctcagtcc gaacatctcc aaaaaacgtg ctgttgtccc ataaaggtaa gggcgtccca
    10141 attcctccga ccgccctacc acgcggataa gcccacgttc cagaagctgt cgaatcacct
    10201 cgccacattg gactccccga atcgattcga tctccgcccg taagattggc tgacgatagg
    10261 cgataactgt cagtgtctcc aaagcgggtg gcgacagtcg cgtctggtgc ggatccttca
    10321 ccagccgggc gatccatggc tcaaaacatg cccgagtccg tagttggaat cccccggcaa
    10381 cctctattgc gcgaaacgcc atcctctttt catcatacaa cttgttgagt cgtcgcaaca
    10441 acgcccgcac ttggctaccg ctatccaaac ccgcaatttg ggcaattttt cttgccgaaa
    10501 ttggctgccg cgccaagaag aggacggctt ccacccgggc taattggtgg tcacagtcgg
    10561 aaaactccgc tgaaaatgca tccgcaaccg gttgccgcga aacgtcttct tcaggtgaga
    10621 gtgaccacaa caacttcgtt gcagcggtag tcggtgggtg caaacgccaa aagcggtttc
    10681 cctgtgccat cgcaccgggc cagccaccgt aacaagttgt ccaaggattg tgccaccagt
    10741 tcatctattt tgttgacaac tcttgacaaa tggcgtctca ctgacttccc aatatcccac
    10801 ccgctgtgtc tgccgataaa tcggcattgc agaagcaaaa actccagtct aggggacacg
    10861 tcggacgtga tgcaagggtt tcgatggccc tgaatcgggt cgttcaggga tcagcggggg
    10921 actggcgagg catcctggag ccactgttcg acttgtcgca ggacgttttc catggcctct
    10981 tctggccggg acgccacggc ctcgaagaac ctgacggtcc cactcgattg cccgaccggc
    11041 atctcgcacc agaagcgata taattccccc cgatttcccc aggtttccaa ccgatagcga
    11101 accgcgccaa gtttctcaaa ccgaaacgcc cagtcccgaa gacctggctc ttcccccctg
    11161 taaggtggca aagacacaga tgggccaggc gacattgtct cgacgctgcc aaaactctca
    11221 agggtcgcac tcgggctaac cattgtgtac gcacgttggc caccacctgc ttcagacgtg
    11281 gcttgatagt cccaactcgt aaaaccagag gatgttacgc cgtcgagtgg ctgcacggat
    11341 tggattgacg gcgacggggt cgcttcatga tggaggcaca ttccgaatga ccgcttatcc
    11401 tctaaagttt cccttattgg cgctgccgtg gctggtccgg agttgtcatc gaggcgtggg
    11461 tgattctcca ggtgagaaag ggcactctgg gtgactggcg cgagggcgac agtggcctgg
    11521 atggcttgag cctgagtttg gctacccatg acgaatcgcc caggcggggt ttgctcattc
    11581 gccgccagat tgaccgtagg agacgcgtca gggttctttg ctgggcacaa gctaggtttt
    11641 tcgtccgtat gtaagagaac gagggccggt agggtgcctt ggggtatcga ggcaagagcg
    11701 accataccaa agagcaaacc aaacaagatg agggctctga gaacctcaga aaacgacatc
    11761 ttggggcaca cttgggccat ttcgacggtg gtttcgttat caaaatctgt catttttcgg
    11821 tcgaggggtc tctggaaaaa tgcgctgaat gggcgaagat gtggaaacat catactggcg
    11881 gacgggacca cgaggcaaca tcaatatcgg cattggaatg gccattgctg catcacgggt
    11941 ctgcgagtcg ttcgatggac atcggccagc acgaggccat gggcgcgatc cctgaacgac
    12001 tgtcgagaaa cgggcgtgcc acaacctcat ggtgaggcgc ggaggatgag atgtatgttc
    12061 tttctgcggg acggtgcctt ctagtttgct tgtcactttt ggggacagca tgtcagtttt
    12121 ttggacaggc ggaaaaccat aactcggtgg accaggacca ggctgcgggt gccgggctgg
    12181 aggcccaggt ggaagagctt ctcagtcgcc tggatgctcc gcaacgcgcc gtccgtgacc
    12241 aggcggaaag agacttgatg gctcttggtc ggcaggcaat cccattcttg cagccaaaaa
    12301 ggatgttatc tccagaagcc gcgttgcgac ttgcccgcgt gcgaacgact ctgatcgagc
    12361 agatcgtggc gacggatgcc ctgccaaccc gggtcacatg caaggtgacg gatttgccgc
    12421 taagtgaagt ggttaccaaa atcgcacacc agacaggtaa tgcggtgacg gtgagtgacg
    12481 aggtggcgaa actgccggtc acgtgggatt gtcaagagaa aactttctgg gaggccctcg
    12541 acgaattggc ggcactggtg gatgcggacg tcagccccgc ggaaaccggc ggactagtca
    12601 tccttcccca gaaaaatcga gccgtacagt cgagagtggc agttgcctac tgtggcgtct
    12661 tcagagttcg cctacgggat gttattaaaa ttgagaggca gcacaacacg tcacaggttt
    12721 cgctcgtaag gatcgaaatc gcttgggagc cgcgactcca gccggccgtg atctggtggc
    12781 ggggtatcca catcgacagg gaacaaccgg agagcgcggc gattgaggac aaaactcttg
    12841 cccgccgcga ggtgcccgtt tcgccgcaaa gatgttttat tatcctcacg ctgcctgtaa
    12901 cactcccgtc cgatctcgaa cagaaacctg ccgtgttgcg gggcagtttt gagcctgtgc
    12961 tacccatcgg cgcgacggac gtcgtttttg acttaacgga tttgcaaaag gggcctcgca
    13021 gcgtgatcct cggacgttcc gagatcaccg ccgagcttca acgtcgctcc gcgaatacct
    13081 gcgaagtgag ccttcgcgtt agatatgaag aagcctgtgg agcgtttgcc tcccaccgtg
    13141 ggtggtttta ctcgtggccc gtttatcttg atactgacgg gaaaaaggca ttgccgcggg
    13201 gtgttcaact tcttcggatg accgaagacg gcgtgacctt gctttatcag tttgggaacg
    13261 ccgaagtcac tggaacacgc atggtctgcc gtgtgccatt ggcgattgtc aaaccggtcg
    13321 tggagttcca gttggctttg tctctagagg aacgaaatcg ctccaaagac tagattttga
    13381 aacttccgct gcgttgcatc ttgccacgct tctccgctct aagcgctagg taacgacttc
    13441 cgcacgtcat gggcttttca ggtcgcttct tgtgattgct caacttggtg ggcacagatc
    13501 gcggacgtga agcggatcgg aagggtccca ctgtaaaagc tgttgggcaa ccgaggtggc
    13561 caggtctcgc tcattgagcc ggatcaggca ctcgacgaga tcgcgcgtgg cttggagcag
    13621 tgggcgattg gccgcttgtg taaaaggcag ccgaccgcgg aagtctttcc ccgtggcgtt
    13681 caagcctagc tcataggcgt ggccaaggtg tgctcgcgcg agatctagtt tgttttggaa
    13741 aagggcaatc cgccccaaaa gttgatgggc ctcaagtaat tcgcgacatt cccgcaagag
    13801 ccagagaagc tcgtccacgg caaggtccca ctcttcgtgg gcgatcatgt cgtacacctc
    13861 ctcgatgtca tcgcggagca ggcgggcttt ccgcggatac actaactcgt aaacatcatc
    13921 ctggatacgg cgaacgctga ttccttggga ttgacccgtt cgtctttgag aatctgtttt
    13981 cttgcgagag cgtcctgtgc caggggaacg gcgtccgttt cttttgccgg gtttgcctgg
    14041 accttccgat tggtgatccc tcatttgcga actccgcctg ttgattccga aatgaaactg
    14101 ccgatggcga tgtacctagt gcatggaaga gccagcctgt ttggcatctg gccctaaaga
    14161 taaaggtcat gccgaagcat ttgcaaagac aggtatgtct atccatcatc ctgaagtata
    14221 acagcaacgt ggtaacctag tctgtcccgt gatatttgag gcagaagcac cagcctatcg
    14281 aaagtggcct tttttctagg ggttggtatt cgcgtcttgt gtttgagtca gagggaagcc
    14341 acaatagagc acgaacgcga agcagtgttg aggcctttgt ccggaatgga gggaggttag
    14401 tcgtgaaatc tatcgtcgtt ctcggtattg tccttacttc atccatgaca gcgatctgtt
    14461 tggcagagtc accaacaagt ttcactgtgt atccgattgg ccgcgttgaa aagaccgatg
    14521 gccgatgccg aatcgtcctg gacgaaaaat acaagcccgg tttgctagga ctggagcaat
    14581 ggtcgcatgt ccaagtcctg tggtggtttg acaagaacga cacacctcaa aagcgcgcga
    14641 ttgtgcaggt ccatcctcgc ggcgacaaga acaatcctct gaccggtgtg tttgcttgcc
    14701 gtgcaccggt taggccgaat ctgattgggt taacactttg caaaatccta cgcgtccaag
    14761 acaacgtcgt ggaaatcgat gacattgacg cctttgacgg cacgcctgtt ctggacctga
    14821 aaccatatgc aaaaggcgaa gacacggcag acgaggtccg tgtgccggag tgggccaaaa
    14881 gagcgccacc tcgtaactga tatctccgca gagtgaaaag acgcatggag ttgtgaattc
    14941 cctgcggaac tacgcaagcc atggggcgga aacggtaatc gggcaaagac gatcagaatt
    15001 cgccattcga agcactacgg tcatgtaaga ttcgtattgt agcttgtgca ccccgtgagg
    15061 tgccttgttt gcaacctata ggaggagcgt ccatgagagt gatttttgca ctgctaatag
    15121 tcgtcgcctt gacctgtcca gtttgcgctg aggtcgttgt gtatccggcc ccttctggtg
    15181 aagaactctc ggcagattac cgcgtttcag tggccgggca agacgtcgcc gtctataccg
    15241 ccaggacatt ggatccgcct tttgctggga aacaatggga ttacggtgga ccgtactcgt
    15301 ttgccagttt tgataccgat gagccggtgg aagtgagaat cacttcgccg agggacttga
    15361 ggaacactgt gattcgtccg tcctatcctg atgtcacctt gcacgtggtc aatgagcacg
    15421 aggtcgttgt cagactcccg ggcccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnncattg gacgattgtg ccggttcaca gccagcgggt tacgatccgc aacgtgaaga
    15601 tttgcggtgg tcgtgtccaa aatgatgacg gaatcaatcc ctgcaattcg caagacgtgc
    15661 ttatcacgga ctgttttatc cgcagcgacg acgactgtgt tgccttgaag ggcttggact
    15721 tcacggaggg taacaacaac gtggaaagaa tccgggtcga gaattgcatc ctgtggtgtg
    15781 acagagcgag gatttttctc cttggtcatg aaagtcgggc acccttcatg cgcgatattg
    15841 tcttgaccga tctggagatt atccatttct cgatgacgcc ttttctcttg gaaccgggtg
    15901 aagacatgcg tttggagaat gtcctcgtct tgggtgtccg gatacatggt gaaggccagc
    15961 aggagctcat tcgcctacgt cccgtagtta accagtacat gagaaaaaag acaccaggtt
    16021 ttattaagaa tgtcacattt aaggatgtaa cggtgtatgg tgcccctggg ggctaccgcg
    16081 tgcaaatcca cggagcggat gccgagcata atgtgcgtga cgtgacattt gataacgtcg
    16141 tcattctgga cgagaagctt acgggcgaat ccagtcgatt ggaaaccggc ccacacgtgg
    16201 aaggcgtgac atttaagtag ccccgcacgt ggacatcgcc aagtagctct gtttggggtt
    16261 tcgaggacgg ccctctgaag tctgtgctac cgtctcgttg gcagctgcca ctcacctgtg
    16321 ctgcctgcag gctaaacgtc gcaggggaca ccctagcgac ctaccaaagc tggctaatcg
    16381 cttctacagt ccgatcgacc aggacttggc caccttccgg gccgatgcgg ccactttgaa
    16441 tcactgcgcc gtagccacct ccctgaacag cccgagccgt gggcaaatat cctcccgatc
    16501 cgacggccaa ctggacgaca aatgtctgca cggccggact acgggccttg atctgaattc
    16561 cgtaatcggt gtaaagctca aacgggttcg tggcaatggc gatatctcca aggcgaatca
    16621 catgcaattc catggggagc gctccaatct cgcccgactg ctgcttttcg taaagatcca
    16681 caaccctttg atgccacaag tagttccagc gctcagacgg tttgtccgcg tactccgcgg
    16741 ccaatttgcg tgcttcttct gcctcggcct ctgtcacccg acggtaagga agctcaattg
    16801 tttgcaccac atgctttagg acgacgtcct ttcgcatatc cagtttggct acctcatagg
    16861 cctcctccca accacggacg attcgccgcg cgatctcgtc caatcgcgac agatttcgaa
    16921 gctttcgcat cctctcgtcg gcggctttgc catacataag atgcggcgtg acatcccccc
    16981 ctgccccggt caatcccagg atggccaggt ctgacccata acggctgcga aggagctcac
    17041 gtacgggatg ccaaaaatca gcatgaatca cggacaggcc ttccacttcc tgggcgggac
    17101 aggcaatatt gatggccgtg gcgatgagct ttccctcatt attccagaaa aagaggacct
    17161 caagattgtg gtcttcctgg ctttccatcc ctcggaactc ggggacattg gtggcgccat
    17221 acatttttgc ggagccatcg gcataaacgg cacgtcggtt ttgggcgacg acagcctggc
    17281 cttgtcccca tcccacttgc gcaggttggc gtttttccca tgcctgcccg atagcttggg
    17341 caatctgggt cgtcatccaa tcggtatatg cagaaggacg catcacccca gtctctggca
    17401 gggtgtattt accctccaca gtaaccgggg cggtatgtgt atgcgtggca caaaggaata
    17461 tcttgagggg atcgagatca ttcttaagcc gaaccacctt ctcccgcacc atttggcaaa
    17521 caccctcgcg aatggcgaca agatcgcaag acacaatcac ggcctgatcc aagacttgtt
    17581 ccccttgtct ggattccatg gcgagcacag tggccgtgat gggtgtggcc gcttctttgg
    17641 cgattcgcac gtgtcgctgg ccatcaagag caaccggctc ggatggcgtg atgtccacga
    17701 cactcagtcc aacgtaaagc tcctgggcca aagcaagttg cgagattaac gaagtcgcaa
    17761 caaggaccgt ccaaaaaaag tggcggccat tcatgactgg ctccttatct acagaaaaaa
    17821 gtgcaactaa tcggctgact gtgcgtctct gatgtgggtc ccttatcgtg gtgccatcat
    17881 gctagccccg tacctaatgt ctgtcaatca ttacagcgac cggtcccgac gcatctcgct
    17941 tgccggaggt cttgccgatt cgcttcattg gtgttatcac aagatagccg caaatgagcg
    18001 cttccgtcgc tgggtgacat gggccagtca agttgtcaaa tgagcctatc gtggcccaag
    18061 aatcatctct ggtaaaaagg agtctggcga tgaaatcaac tcttttatgc gcggtcgtca
    18121 tcttcgtcgt cttccgaatg ttgccaatag gattgtgcgg tgagatacgc tttcggcacc
    18181 actttgtgga tcgagatctc ccaggcaatt cgtggggcca aacggccgtg gccgacctcg
    18241 atcgcgacgg acgtttggat tacatcaccg gtaaaagtcg tggccagatc gtttggtacc
    18301 ggcaaatctc tttgacccag tgggaacgtc acatattggg tgaggattcg ccctcggatg
    18361 taggtggggc cgtgttggat gtcgatggcg atggttggat ggattttgtc acaggtggag
    18421 cgtggtatcg aaatccgcag agacctacgg aagggccttt cgaacggatt gtctttgaca
    18481 aagaactcca aggggttcat gacgtggtcg ttgcggatgt aaacggcgat ggccgtctcg
    18541 atgtgcttac catgtctgac cggaacaata tccgttggta ccagattccg gaaaatccgc
    18601 gggaactgtg ggtgcgaacc gacattgggc catccgtcca cgcgggcctg gccgtcggcg
    18661 atttggacgg ggacggcgat ctcgacgtca tccgctcgaa tctctggttc gaaaatgtgg
    18721 acggcaaagg aaagacctgg aatgtgcacg aaaacattcc cttcggcaat cccagcccgc
    18781 cgtacgcttt ggcaacccga tgtatagcag tcgatatcga tagcgacggc gatctggatc
    18841 tggtcatgac cgagaatgaa atccgcggag gacggatcgg ttggttggag aacctgggat
    18901 atggcaaggc gtggaacctt catctccttc cccaaggtga tgcgagcatt cgcggggcct
    18961 accattccct gattgtggcg gacttcgatc tcgatggtga ctgggatgta tttagctgcg
    19021 aaatggaagg catcatgggc gagaaattgc ctcgttggtt tgtttgggaa aacgtggatg
    19081 gtaagggaca aaattgggtg gagcacgtgg tcctagatgc cggccttggt ggacacgagg
    19141 ccgtggccgg cgatttcgat ggcgacgggg acttggatat catcagtaag ctttggcgcc
    19201 cgcgcaagga taatgccaac ggcgggaaga accacgtgga ccttttggaa aacctccggc
    19261 gataaatgcc gcgaaaaagg ggtgttgatt taccgtgatg aaccaggttt gcggctggcg
    19321 gcgggatttc agctcggcaa ttgccaggcg gtttgtagcc cattagcgat cgcatttggt
    19381 gtgggtatgg acgtcatcgc tacccttctc ggcaattctt tgctgttcac ggatgaaacg
    19441 gcgcgcttcg gcccgtggcc ttgaagtgct ccagtaagcg cttcaactcg gcggccaggt
    19501 ccgctttttg gcctgccaga ttgatccgtt cgcccggatc ttctgccaga ttgtaaagct
    19561 ggcccggtgc gtccggtgca gtgtcctcga tggccagtgc tgccagggct ccgcgatcat
    19621 aacggttgcc accggagcct ttgtggtcga gatacttcca cgctcctcgg cggattccca
    19681 gagccagact gatcgtctgg tgcaacacga tgcgatccgc atcctctggg gcggaacggc
    19741 ccagcaaggc gtgcaagaag ctgttgctat cttcggcagc gttgtcgggc agctttgccc
    19801 cgacgatctg ggcacaggta gccatgatat ccgtgtggca gatgggcagt tcgcagatgc
    19861 tgcccggttt gacccgcccg ggccatcgca cgatcagcgg tacccgatgg ccgccttccc
    19921 agttgtcacg ttttagcccg cgccagggtc gggcgccatc gtggccgtag tcggcccgca
    19981 tgtgcaacac ggtggtcact tccggcccat tgtcgctggt gaaaataacc agtgtgttgt
    20041 cggccacccc aagccgttcg agcgtctgca tcaattggcc caccacatag tccagttcga
    20101 agatgaagtc gccgtgcggc ccggccttgg tggcgccttg gaactgggcg gcgggaaacg
    20161 acggcagatg caccgcgctg gtggcgtgat agaggaaaaa cggctggccg ggatgttcgc
    20221 gcacgtgacg ttcgagccac tgccggcttt tctccaggaa taccaggtcg agttcttcca
    20281 ggttgtagcc cggtgcgatc aggcccggcc gattgtcctc ggaataggga tgctggggga
    20341 ggccgcttcg gtccaagggc ttggtggggg gcaccggtat gcgatcgcct tcgatatagg
    20401 catagagcca atcggtaccg ggacaacagg ctgtgccgaa gaagtagtcg aagccccggt
    20461 ggatcggccc gtcggggatg ggccgcgtat agtcgatccg cttgaccgct tccagcccgt
    20521 cttgatgaat gggccgaccc tccttgtcga aaaacgtcag ccccacgtgc catttgccca
    20581 tgcaagccgt ggcgtagcct ttgttgcgca gcaactgcgg caaggtcagt cggccttctt
    20641 cgatcaggca aggaccaccc gcaccagtga acacgccccg catgcccgta cggaaacaca
    20701 tgcgcccggt gagcaaactg tagcgactgg gcgtgcagac cgtggccgca ctgtgggcgt
    20761 cggtgaagcg catgccctcg gcggccagct tgtccagatg gggcgtgggc accttagact
    20821 cggggttgta gcaaccgacg tccccatagc cgaggtcgtc ggccaggatc aaaacgatat
    20881 taggcagccg gccggcaggt gcttgtgcat cttctacgcc gaaagccgtg taacttgaag
    20941 tcagcagcac cgcaccgagc agtgcgaccg accacaaaca atcaaacact cgtcccccat
    21001 cccgcaaccg tgaaggccac agccaacacg gtgaatgagt atttgggaag ctcaaccggg
    21061 cgatggtgcg aagcgaaaca ggcatgtgca ctaaattgag caaatacata tagcaagcct
    21121 tctagacatg gttttcgtga cagttcacgg cgtgttactc ttgtcgtgcc cattcttgtt
    21181 ttcacgccag tgggtcggca gtcctgatgt tgaagaggca gttttggctt gtcaagcccc
    21241 tcgtgattag atttgttgcg tacccggagg cacgcaatgg gtcgtgattg accggccgga
    21301 gatccctagt tgccccctta tcaagctgcg gcaaccgagg tccgactgcg aacatgaccg
    21361 gttggcctaa ggcatgacta agctcgtggg agagctcttt tccgtcccgc aacgcaactc
    21421 acacaaggga cgcggagtgc gaacgtcctg tggatggtcc cctaccgccg ttggtgtaag
    21481 cagatctgca tggtgatcgc cacctgcgtc aaacagggcg gtctttcgct tcctgctcga
    21541 cctgcaatag gctcagctcc gcggtacgct tccaggcccc gtcattgccc gcagctttcc
    21601 gacggcgcgg tcggttttct ggtatgctgt tgttacccac ggtcgccgta catccaccgg
    21661 gaacgctcac cacaaatcgt tcatgcccgg attgcgattg attggagcgt caaataggat
    21721 aacagaacgt tgtggagcca attgcgatcg gcactgagaa caaagatggg gaggttgcca
    21781 tgagattctt gtctggtctc ttcgccgtat tgatttgttt acacacagta agcttggcca
    21841 ttgagccggc caatcctaac ttgtgtcaag agggtaagga acttcttgcg ttcttcgaga
    21901 gcatttacgg aaagcaagtc ttggcgggct acaacgttta tccacatacg ccggacgact
    21961 atcaacaaac cgcaagacag gcagctattt gggggcgtga catccgttgg cttggcgatg
    22021 tggaggaggt agtatctcat gtccgcgcac atggctattt cctgacgcta cactggcact
    22081 ggttcttcga cgaggattcg gcatggacca gggagcgcaa aaagccggtg gatttggcaa
    22141 aggtgatcac cccgggaaca caggaacatg agaccctcat gaaggaactg gcagccaccg
    22201 ccgatgtcct ggagcgactt caaaaggagg gaatcgtcgt cttgtggcgt ccccttcatg
    22261 agatcgacgg gggttggttc tggtggacga atttgaacca tccagagaac accgcggaat
    22321 tgtggcggat catgttccgt tactttacgg atgagcgaaa gctcaacaac ctcatttggg
    22381 tctatagtgc cggagtgggc gatctgaaga agaaacctgc cgaatggcga cggcgatttt
    22441 atcccgggga tgcttacgtc gatatttcgg gaattgatat ctatggtgtg gacccgaaga
    22501 atgacgagac tccctattgg gattactaca aactcatgga ggaggtttca cccgggaaga
    22561 tgttggccct ttgtgagtgc gacgccatcc ccaatcctga caagatggct gccggcaaaa
    22621 cgccgaaatg gctctacgcc ttgccttggt ggggctgtcc ttccccacgc cgttccgtgg
    22681 aatgggcccg tttcacaatg ggtcacgagt tggttttaac tctcgatgaa atacctacga
    22741 tggggcggaa agatcttgtt cctcaaattg gcatcgtaga gcctaatgat gacggcagcg
    22801 cgtggtttcc aaaaagcgag gtgactatga gcggctatgc agtctcacga ggtgctgccg
    22861 tggcccgtgt agagttcctc gctaatgacg aagtaatcgg cacattggaa aagccaccgg
    22921 cccatttttc gtggtcctgg gtaagtccgc gtgcgggaac ttttgaccta accgcaaggg
    22981 ccgtggacac tttgggccgc acaaacacct cgaatcgcat tcaccttgcc gtgggcgtga
    23041 ccaatgcagc caagaataaa ccggtgcggg tttcctccgg cgaggatcca caggccgccg
    23101 tggatggctc atattacacc agttggcgag ctgccaaaga tgccgaagag gccacttgcg
    23161 aaattgattt gggtcaagcc atggcggtct cgcaggtgaa tctcatctgg ggatggaaga
    23221 ttcaccccac ccgctttgtg atcgaagtcg gctccggtga gagctgggat caggtccgcg
    23281 attggaaaac ggtggccacc atggaaaatc tgccatgggt gacgtggaag gcgacccatc
    23341 gtgtgcgttt tgagcctgtc accgcgcgat tcgttcgcat tcggatgttc cagcgagcaa
    23401 accggcagac ctggtcgggt tacgacctag cggaaattga gattcccgta cctgtcaaat
    23461 aactctcaaa aatgtaggga gccgccttag atcttaaaaa gtccccctct ccctccggga
    23521 gagggttggg gtgagggcaa gcgatcgtcg gaatcgattg agaacaccgg cagcgattcg
    23581 tctctccccg ccgaacgcgt gagagggagg gtgggatagg ctcttaagtt actgctcttt
    23641 gcccatcgct tcggcttgct gggcttggta ctttgcccaa cttgcgtacc acggcagggg
    23701 tgtaaatcga cgggcaaaga tgtgccggag tggtggtagc ccccagacct tgcggtgaag
    23761 ttcctcctgt actgcccaga aacggtcaac ggcggccaac cactcgggcg tccattcgga
    23821 gccggttccg atttcgcggt ggtggcgaaa cagttcgcac tttaaccgcc attgttccca
    23881 tcgccaattt gacccacacg cggagcgagg ttctggccga ggcaattcct gaagggactg
    23941 cgcggcgacg acaggatcga cgctgaacgg tcgtccccat gcccgaagcc actgagccca
    24001 cttctgagcg ttctcgcccg tggcgtggaa gtatcgctcc gcataagagg tcagtacggc
    24061 atggctatcc gagaattgac cggaggacaa ccccgccaaa agggccttgt tcacgtcgtc
    24121 aaacacacct tccgaatacg ccataacgcc agtacaacca cgcgcggcga gctcttggac
    24181 ggtcttttgc aggcgctctg ccgcgacgac cggcccgaga tggccatagg tatcccttgg
    24241 gctggcttcc tcggcataac caatgtggac aaatgcagaa atctcacaac cggttggcag
    24301 cggtacatcg gccacggtcg tttggccgta ggggatgtgg aggtacatcc gttggatcca
    24361 accctcacaa tgtgtattga cccaatcagc aaacaggcgg tgttcttccg gttcccacca
    24421 ccagccgatc atgtgcattt ccaggttggg gtggtatttc tttccgatat cgtagatctc
    24481 cttggacaac tgtgcgaaag tgatgatcca cggtttgcaa cgctcgcaag cacaaccgcc
    24541 gaaatcgtac ggggccgaac aaagagccga caggcggaca ccgctgcggg ccaaatcggc
    24601 aaagagattc tcgtaattcc tgagaatgac ttttcgtgcc tcggcatgag aagggcaaat
    24661 caattgccca aagattcgac ccccttgtac cgctttccat tgatccttgc actgatcgac
    24721 atagacgtga ttcggagtga ttaccaaatc acacgcaaat cccagttttt gggcaatcgc
    24781 aaagtttgcc tttttacgat cccacatggc gtgcgccagt tggtaaagac gcgtttgcgc
    24841 aaaaggatcg gagcaatctt ccatgtcgaa ccaatcagca tatcggttaa agccccagaa
    24901 ctgggcttcg
//
LOCUS       DAOSUT010000052        24661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1328_length_24661_cov_8.919938, whole genome shotgun sequence.
ACCESSION   DAOSUT010000052 DAOSUT010000000
VERSION     DAOSUT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24661
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_24661_cov_8.919938"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLD05_06590"
     CDS             <1..593
                     /locus_tag="PLD05_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77139.1"
                     /translation="GNDQFVATPTYAEMTGTGFKLRAEGFRYAHGYAANGGTDTAMMY
                     DSAGDDEYVCTPDYNILRGDNFYIRAKLFDYVHAYATAGGYDKATIKGSAGDDKIVLE
                     PNVVRLNSRSYVHRGKFFEEITVRGGGGRDIAYVYDGVVLPGTVARGTPPVPLDASQI
                     VYLRQLYKIQTYNEGGTGTSRTIRPAVDAVFSAYWP"
     gene            1076..2365
                     /locus_tag="PLD05_06595"
     CDS             1076..2365
                     /locus_tag="PLD05_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HOP77140.1"
                     /translation="MYCQRKVMRTIVGVMLAVLATPALAQVKDMQCFAPAQVESFGGG
                     RRANEGFFISGEYLRWSISKPDVTTVGYPGLSREVYYDTGISRIQTNTLDTSFIGSKF
                     TDGSRVDLGYIEGHEGWLFSGTFLSTLGRTYGVASADIVFDDPPFGPPPTYRLLQGYV
                     DDALTDIQNLPVTFTAPYIEDLEPDEGGDPDTLPEPSWGVMMENLVKHWSTELNYIYR
                     FHPTRRGTTWELFLGVRYFELNEDFNVWAPYSYVPYDPTADENDPDGVGFPTRVGILA
                     DSYWFTKAENHVVGPQIGGRFFRSYNRWTFDVQARFFAGFNFQNIKQHGVLGSRLNPS
                     ADPIEDVPLFMGPTGFSHSASEEEWAPCAELRAGIKYQLTSKLSLSVGWTGLWMDNVA
                     RASNMILYRVPDMGITMDHNTQDLFINGVTAGVEFNR"
     gene            2784..3323
                     /locus_tag="PLD05_06600"
     CDS             2784..3323
                     /locus_tag="PLD05_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HOP77141.1"
                     /translation="MLSVRPRVTELAFVLYGRPLHPELFEIRATRHIARGDYQARLDI
                     TNTGHIITWNYEGLTWTEVATAAHHPLPAKRRLMFHNIKGELRDEVIGRGGVRYEWEA
                     QLEHVSPEVFWVFQHELNREAPQQGLFFRFDPSGRMPLGAMSFLYFETRAKSLLVQAF
                     HTFPDDCAVVKSKTVIRLP"
     gene            complement(3401..4216)
                     /locus_tag="PLD05_06605"
     CDS             complement(3401..4216)
                     /locus_tag="PLD05_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOP77142.1"
                     /translation="MRIVSLPDKLSEGNTFSMAVEDQGQGRTILLVHAFPFDHRVWEA
                     QIKGLSTRFRVLAPDLRGFGRSTVTPGVVTMEQMADDLAAMLDALAIDEPVVLCGLSM
                     GGYVAFQFVRRHRQRLRGLILCDTRAASDSQEARENRLRTAERVITEGTAFLADQMLP
                     RLCCERTFRHQPEIVTKLRTMILEAPPEGVAAAARGMAQRPDSTPLLGQIDCPTMVVV
                     GQFDAISLPEEMEAMARAIPGASFSVVPDAGHMAPLEQGEHTTQLIAEFASRL"
     gene            complement(4392..6503)
                     /gene="glgX"
                     /locus_tag="PLD05_06610"
     CDS             complement(4392..6503)
                     /gene="glgX"
                     /locus_tag="PLD05_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOP77143.1"
                     /translation="MLARPLPLQFAHHLPYGAILHEDGVQFVIFSRSATAMRILLYND
                     VNDREPSDIIHLNPADHRWGDVWNVFVPGLKAGQLYHIQADGPYAPEIGHRFDPKARL
                     IDPYAKALAGDFLPATDGIIRPPKCVVVDDTFDWQGTRHIRRDLSETIIYEMHVKGFT
                     RSPTSGAEFPGTYLGVIEKIPYLKSLGITAVELMPVHEFLILDCYGQKPKRPNYWGYD
                     PLAFFAPHRGYAVSKEPGAQVREFKEMVRELHKAGIEVILDVVFNHTAEGNEYGPTLS
                     FKGLENRVYYMLENGGSRYKNYTGCGNTLNGNHPIVREMIFHCLRYWVHNYHIDGFRF
                     DLASILSRDRAGNLVPNPPVVETIAEDPLLADTKIIAEAWDAAGAYQVGTFASLCWPD
                     TFAHCRWAEWNGRYRDDVRRFWRGDPGMVPAFATRIAGSSDLYQPGGRYPYHSINFIT
                     SHDGFTLNDLVSYNHKHNEENGEDNRDGENNNNSYNYGVEGPTWHKGINLIRLRQIKN
                     MMATLLLSQGVPMILFGDECRRTQRGNNNAYCQDNSISWFNWKLVHRHRELWRFVQAL
                     IAFRRREPTVRRKDFLTGLPVRPGGFPDVSWFNPAGGPVDWTNAGRSLTCFLAAFPHR
                     EDPNRPNHHILLMFHAAPEPTRFVIPAMARGLAWRLFIDTSRMSPNDIYPDLDGPPPP
                     VDWTLNVPGRTLICYVAPDLL"
     gene            7409..7753
                     /locus_tag="PLD05_06615"
     CDS             7409..7753
                     /locus_tag="PLD05_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77144.1"
                     /translation="MLAKKVNTPQMGSLGRGRKRCPSCNAIVAARAVKCRFCEYNFLD
                     KPRRGKTARGTVADPASVEKLVAFINAHGGLENAKKVISDVRGLVGYLGSLDLIAQQL
                     NVVGLIKQQVKE"
     gene            complement(8062..9963)
                     /locus_tag="PLD05_06620"
     CDS             complement(8062..9963)
                     /locus_tag="PLD05_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77145.1"
                     /translation="MYRHVFGSLGAILALWFGLTLASHRTIWAEQARPNILWLTCEDM
                     GPHLGCYGDRYANTPNLDRLAAKGTIYTRVWSNAPVCAPARTAIISGMYPSTTGSEHM
                     RSLVPTAPGLRFYPQFLRDAGYYCTNNSKEDYNLEKPGQVWDDSSGKAHWKNRQPGQP
                     FFAIFNFTVTHESQVRRRPHEWVHDPAKVRVPAYHPDTIEVRQDWAQYYDNITTMDRM
                     AGEKLKELEDAGLADDTIVFFYGDHGPGLPRCKRCPYNSGLQVGLIVYIPPKFRHLAP
                     PDYQPGGRSSRLVSFVDLGPTLLSLVGIKPPASMQGRAFLGTFATEPPRYMFGLRGRM
                     DERYDLVRSVTDGRFVYIRNYMPHLPWGQHVEYLFQTPTTRVWKQLFDEGKLSPEQSY
                     FWTIPKAKEELYDLATDPDEVHNVANNPAYKAKLEELRQVLRDHILTTRDLGFLPEDE
                     IHARAGSKTPYEYGQSADYPLERILTMAEVASDLEEDHLEQLLAGLQDPDSAVRYWAV
                     TGLLARGEKVFSAVHDRLVKALEDPAPSVQIVAAWTLGRYGSQQDVELALSVLRTYLD
                     YEGKGPYLMMLALNAVDNLGTKAAPLREIVAQIEPKIQPKHGRLGENLNKLKRHILSH
                     FEVAGKEQP"
     gene            10277..11932
                     /locus_tag="PLD05_06625"
                     /pseudo
     CDS             10277..11932
                     /locus_tag="PLD05_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            12061..14484
                     /locus_tag="PLD05_06630"
     CDS             12061..14484
                     /locus_tag="PLD05_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOP77146.1"
                     /translation="MKKRNVFLVSVGYLMVLAAICGGAVGAESAQPRRLTIVPFTRVH
                     AEDTFWAVRIKTNRERSIPHNLKWCEDTGRISNFAKAAGLMEGKFEGIYFNDSDVYKV
                     LEGASYALAQQFDADLASQVDRIIGLIAAAQRPDGYLNTYYTLTNLNDRWTDLPVKHE
                     LYCAGHLFEAAVAHYRATGKRTFLDVAIKLADHIDSIFGEGKRYGVPGHEEIELALVK
                     LYEVTGEKRYFDLAKFFIDERGNTTHRPSYGPYSQDHLPVRQQSEIVGHAVRAMYLYS
                     GVADVAAYTGDPELIAAMDRIWKDVTLRKMYITGGIGARHEGEAFGEPYELPNESAYC
                     ETCAAIGLVFWAYRLGLMHGDAQYADVLERALYNGVLSGVALDGEHFFYVNPLASGGN
                     HHRQPFFGCACCPTNVVRLIPSVPGYVYAVRPPTGEKPAELFVNLYLGSTAEVELEED
                     NVVKVTQETRYPWEGRIVLTVTPELEREWSLRLRVPGWCREARLAVNSEGPTPVDLTK
                     LDRGYHLIRRVWKKGDKVTLDLAMPVERIEAHPKVVADRGRVAIQRGPLVYCLETVDN
                     GFDVRTAILAKDPQFETEFRPDLLGGIVVIRATAADGRRLTAVPYHLWDHRDPGPMVV
                     WIRQAGKSRYPDPEDPAWEGRLYRPVDPTSLTAEDSPTVMEAARPSASHCWSYDSVEA
                     LNDGIEPRNSNDHSIPRFTWWDHRGTQEWVEYEWDKPVALQGVRVYWFDDEPRGGHCR
                     LPASWRILYRKGDAWVPVTSQSELPIVKDGWSEASFGPVQTQGLRLEVQLRPNWSGGI
                     LEWQVIEAK"
     gene            complement(14670..16535)
                     /locus_tag="PLD05_06635"
     CDS             complement(14670..16535)
                     /locus_tag="PLD05_06635"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HOP77147.1"
                     /translation="MLRSLAALLVFLGVTRQVFTQRQMEDLDRGLVAIDRGDGTVMVS
                     WRLLADDPDDLAFHVYRRQEDGTWIRLTDSPLLDRTNFVDEDRDSPWPRFYQVRNVIS
                     GKETGETATVEVAKGQTHWVIPLQTLPGYTPNDASVGDLNGDGRYELVLKQEKRPRDN
                     SQRGPTGQTLLEAYTFDGKFLWRINLGPNIREGAHYTPFLVYDFDGDGRAEVVCRTSD
                     GTVDGTGQVIGSPSANHRNADGLVLTGPEYLTVFEGHTGKAVVSVPYVPPRGDVRAWG
                     DDYGNRSDRFLTCVAYLDGRQPSVVMCRGYYTRTVLAAWNYRDGQLSLVWVFDSDTDP
                     RENRKYRGQGNHNLSVADVNGDGRDEIIYGACVIGPDGQGLYSTELGHGDAMHVTDID
                     PHRPGLEIFSIHERPSHPFGANLRDAATGEIIWGLAARDVSRGLALDIDPRHPGLECW
                     AVGPGLRGLFNCRGEQIANRAPASCNMGVWWDGDLLREVLDRTQIQKWIPEREACDVL
                     FDAARYGCTWNNGSKANPTLCADIFGDWREEVIWRSADNRSLHIFTTPLPSEHRLVTL
                     MHDPVYRLGIAWQNVGYNQPAHPGFYLGHGMTPPRRAKIVTPKALGQSPDRPQTD"
     gene            16929..17681
                     /locus_tag="PLD05_06640"
     CDS             16929..17681
                     /locus_tag="PLD05_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77148.1"
                     /translation="MIEFLCPNGHRIRCSEDRAGKPAKCPKCSVKFLIPTLEEIRAAQ
                     GEDAGSNATFSRDRTRIQFLCPNGHRLFGSADLQGRPGQCPECGVRFRIPVIEELTDD
                     DADEAVPLSVAPEPSAGPQLATGTGLGDSAIVPESGIHLELFSPRSAGDSRTPESSIS
                     SPTSIKGSHLARIFERLWNGRAAGSVVKVQLVDGQAFTVTKYSASSNDHEYAAFVVEG
                     SAFGGGLVVVPWQSVRMIYVSGVKDIPSQLLD"
     gene            18207..20501
                     /locus_tag="PLD05_06645"
     CDS             18207..20501
                     /locus_tag="PLD05_06645"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOP77149.1"
                     /translation="MSELIRVSHPTALSPAVTAAMMTPTPIQRETLHGPPKPIDVFHA
                     VRRRWWLILLLGTIGAVAVMIFAWRTIPVQYTATAWLQVSLQRPRIIFDTREDDPLLR
                     NRQTQATLVTSNRVLGAALRQPGISQLPYIRNQIDPMMWLRQKIRVRFADQAEILEIS
                     MTGEDPEQIAAIVNAVKDAYMAEVGDVNRTYALERKRLLEQNYAQTLELVKKRDLRYR
                     ELANAVGSADSEVARRKAITALETLADHRRRVNEIRQQLGGLDRKITLLQTRLKYLEE
                     HRPQSSEEERRERDEAVIRQIVEAGLRADPWISSAQARLSALEARWFEERQRVVQWEK
                     SPTILRLQEEMQALREAIENRRNELIPGLTQYAREQLEKGRAKAAPTEAELIQAQIAE
                     AELERAVLEDELKVALEQFQKEAAEAEQLGKYSADLEAEKAEVDRLKAVAAKMGDELE
                     RWNVELAAPPRVRVLEEASTPRVSNINDKYRLVTFLGLMTFLGTAAVVVLLDFLLRPV
                     SSAYQISYGLGLPVLGDLPLIQRGPFSRITSNGQMPEHVKTILLESVDQLRTVLLHRA
                     KRDGVKSVLVTSALAREGKTTLASQLAVSMARAGRRVILVDGDLRRPRLHRVLRRDLH
                     PGLAELARNEATPQEIVQAAELEGLYLVTAGHCDAAALEAVSQGRLDGFLDSLRDTFD
                     MVLIDSSPLLTTADSMILAHLADGVVLSVVQDRTRLPRLFEAVQRLRAVDLPLIGIVI
                     HGTAPSRYRSYYRSYTIDVEVRKS"
     gene            20598..22073
                     /locus_tag="PLD05_06650"
     CDS             20598..22073
                     /locus_tag="PLD05_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77150.1"
                     /translation="MKNSWIVVLGLTLFLSRTVFGQAQPQPLNVVFFLVDDLGWTDVG
                     CFGSTFYETPNIDRLAAEGMRFLDAYAACQVCSPTRAAIQTGKWPQRTAITDYIGAPQ
                     PAGWNRNTRLLPAAYETALPLAEITLAELVKRRNYATFFAGKWHLGPEGFWPENQGYD
                     INMGGIDRGGPYGGNKYFSPYGNPRLPDGPPGEHLPDRLARETAKFIEEHKDQPFLAF
                     LSFYSVHTPLMAREDLRQKYEEKRRRLGLEAKFGREGPRDVRLVQEHAVYAGMVEAMD
                     QAVGKVLQKIDELGLRDRTVVFFTSDNGGLSTSEGWPTSNLPLRAGKGWIYEGGVRVP
                     LIVRWPGVVPPGTVSRVPVCSVDFFPTIGDLAGAPWNDIPVDGQSLVPLLKGKPFPED
                     RPLFWHYPHYGNQGGAPSAAVRKGPWKLIHWYEDDSWELYDVVADIGETQNLAQKHPE
                     RVAELRQLLETWLKDVGARFPTANPNYDPNKPDGRIAVRPQ"
     gene            22113..22871
                     /locus_tag="PLD05_06655"
     CDS             22113..22871
                     /locus_tag="PLD05_06655"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOP77151.1"
                     /translation="MNDSLRKLLPAWLAKNGWFFLAVGVIIAAAIVEGHWSLRWGSHS
                     ERVMAPLAQAVMQTPLTVGEWVTDEAVQPDPRVLEISGAEALFQATYRESTQGDVVTV
                     YLLAGWSRDISIHTPDACYPGAGFVMETSPQPFAFSYEANSQAGEQSSTGLRQAEFST
                     AIFTKTEPTGVTRLRVFWSWNDGRGWQAPRFPRWAFGGRRPLVKLYLVSESPPGQPTH
                     ESPALRLASALLPVLDRQLETALSTRPKERMSVP"
     gene            complement(22931..>24661)
                     /locus_tag="PLD05_06660"
     CDS             complement(22931..>24661)
                     /locus_tag="PLD05_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77152.1"
                     /translation="YHETPNLDRLARRGLRFTQAYAANPLCSPTRSSILTGLYPARIG
                     ITSPACHLPQAVLEKGLVPPQPNVRIRTANSLTRLKPEYVTLAEVFREHGYMTAHFGK
                     WHLGHSGPYEPKEQGFMLDFPNTPAAPGPGGGYLAPWKFIKDPAIQGQPGEHIEDRMS
                     QEAARFIEENKDKPFYLNYWCFSVHSPWSAKPELVEYFKTKAKDDSPQRNPVYAAMVK
                     SLDDAVGRIVEAIDKAGLAERTIIIFFSDNGGFAYPPKNETQPPGYAEIPATSNTPLR
                     SGKASIYEGGTRVPLIIVWPGHTQPDSVTDVLFSSVDFFPTILEMCGLPLPGHKVDGV
                     SQVPAILGKGPVRTRVFCHFPHGGENQAQQIPGFLPSAYVRDGDWKLIRFFAGNPDGT
                     DRFELYNLREDIGETHDVAAEHPEIVGRLAKELEGFLQDTDAVIPQVNPDYRGPQTSD
                     PLMGWKNRGCEATIDAGLLKLQAQTATPFLGFGVGKQPGPVQVTFRIKVPQAGEGKIE
                     WIPPGASQDAAQSVPYSVTGTEMTTVTVRVPWEKPIGILRIYLPTGANTAEVDWIELE
                     TASGKRRFDF"
BASE COUNT         5447 a         6394 c         7275 g         5545 t
ORIGIN      
        1 tgggcaacga ccaattcgtt gccacgccta cttacgcgga aatgacgggg acgggtttca
       61 aactccgggc cgagggcttc cgttatgccc atggctacgc ggccaacggt ggtacggaca
      121 cggccatgat gtacgattct gccggtgacg atgaatacgt ctgcacaccg gattacaaca
      181 tactccgcgg ggacaatttc tacatccggg ccaagttgtt cgactatgtc catgcgtacg
      241 ccacggccgg tggttatgac aaggccacga ttaagggctc ggccggcgac gacaagatcg
      301 tccttgagcc gaatgtcgta cggctcaatt cgagaagcta cgtgcatcgt ggcaagttct
      361 tcgaggagat tactgttcgt ggcggcggag gacgcgatat agcctacgtc tatgacggag
      421 tggtcttgcc gggaactgta gcccgcggaa caccacctgt accacttgat gcttcccaaa
      481 ttgtttattt gcgacagctt tacaagattc agacctacaa cgagggtggg acaggcacca
      541 gccgaacaat tcggccagcc gtggatgccg tgttctcggc atactggccg taataggtat
      601 cggctattgg gaaccccccc aatagcggcc taaagtgggg cctggcctca ggcccctcaa
      661 aagggttgtc aaaaacagaa gcccaccatg atggtgggct tcttttcttt tttggtttgt
      721 cgatcgcgaa gagaccaaat tgttcgaccc gacgccgcga tttcgtttgc gagtttgatc
      781 agtttagcga agacgcggca ctgggggaat gtggcggatt gtaacggttt gggcaaacag
      841 ggctgttcga attgatgaaa tgggtttttt cgaagtgacc attgaaaggc gacgaatcaa
      901 ggaaatgctg atgtcgtagg agtgtttggt atttccgtta cgttttgcca ttctttctca
      961 actcttaagg gcgatttttc cgatacaatc ctcgcaaccc catgttgccg gttctccacg
     1021 aggagtgatt gtggcgagga cggaacattt gcaaacattg aggtggacca ggactatgta
     1081 ctgccaacgc aaagtgatgc ggacaatcgt cggggttatg ctcgcagttc ttgcaacccc
     1141 agccttggcc caggtcaaag acatgcagtg ttttgccccg gctcaagtcg agtccttcgg
     1201 aggaggccgg cgggccaacg agggcttctt catcagcggt gaataccttc gttggagcat
     1261 ctctaagccg gatgtgacga ccgtcggcta tcccggccta agtcgagaag tctattacga
     1321 tacaggtatc tcacggattc aaacaaacac cctcgatacg agtttcatcg gatcgaaatt
     1381 cactgatggc agccgtgtcg atctgggcta tatcgaaggc cacgaaggat ggttgttcag
     1441 cggaaccttc ctttccacct tgggccgtac gtatggggtg gcgagcgccg acatcgtttt
     1501 cgacgatccg ccgtttggcc caccaccgac ctatcggttg cttcaaggat atgtggatga
     1561 tgccttgacc gacattcaga atcttcctgt gacgtttacg gctccctata tcgaggacct
     1621 tgaaccagac gaaggcggtg acccggatac gctgccagag ccttcctggg gtgtgatgat
     1681 ggaaaacctc gtcaagcact ggagcacgga actcaactac atttatcggt ttcatcctac
     1741 acgtcgtggc accacgtggg aattgttcct aggcgtgaga tatttcgaat tgaacgagga
     1801 tttcaatgtg tgggcaccct attcttatgt cccctatgat ccgactgcgg atgaaaacga
     1861 tcctgacggt gtaggtttcc caacccgcgt gggtatcttg gctgacagct actggttcac
     1921 gaaggccgag aaccatgtgg tggggccgca gattggaggt cggttcttcc gcagttacaa
     1981 tcggtggacg tttgacgtac aggcccgttt ctttgcaggt ttcaatttcc agaacatcaa
     2041 acagcatggt gtcctcggca gtcgattgaa cccgtctgct gatcccattg aagatgtgcc
     2101 gttgttcatg ggtccgaccg gtttcagcca cagcgccagt gaggaagaat gggcaccttg
     2161 tgccgaactg cgtgctggga tcaagtacca acttacatcc aaactgtccc tcagcgtcgg
     2221 ttggacggga ctatggatgg ataacgtagc ccgggcctcc aatatgatcc tctaccgtgt
     2281 tcccgacatg ggcattacga tggatcataa cacgcaagac ctgttcatca atggcgtgac
     2341 ggctggcgtg gaattcaacc gctagagaac cccgatacgt ctgattcagg attgtcaacc
     2401 gataaaaacg gacaaccctc cagcggttag cgagcggcga aacaaggatg ttgtggccgt
     2461 tcgtctcggg taacgcaaga gggtctttga tcgaccggga aggatgcccg gtcgatcttg
     2521 tttcttggca agggttttcg acgcgctagc gaaatttgtg ccgaaagtca aggtgaattc
     2581 cttgcggtga ttgggagatt gtatgattaa aaaagcggcc tttgtcgctt tcgaacggtt
     2641 gcttataata cgacttagat tgagccgagg acctgtggtc aggcaattag caaagtgaca
     2701 gcggagcctt gtcgcaggcc ttcgtgacac agtgggtcat atttccgaga gtgagtctcg
     2761 gcttaagaag gaagggtaca gccgtgctga gtgttcgtcc gcgtgttacc gaattagcct
     2821 ttgtcctgta tggacggccg ctccacccgg agcttttcga aatccgagcc acgcggcaca
     2881 ttgcgcgcgg ggattatcag gcccgactcg acatcaccaa cacgggccac attatcactt
     2941 ggaattatga agggctcacg tggactgagg ttgccactgc cgcccatcat ccgttgcccg
     3001 caaagcggcg gcttatgttt cataacatca aaggggagct tcgggacgag gtaataggac
     3061 gcggtggagt ccgttatgag tgggaggccc aactcgagca cgtctcgccc gaggttttct
     3121 gggtctttca gcatgaactc aatcgggaag cgcctcagca aggccttttc ttccgctttg
     3181 atccaagcgg gcgcatgccc ttgggggcga tgagctttct ttactttgaa acgcgggcca
     3241 agagtctctt ggtgcaggcg tttcacacat ttcccgatga ctgtgccgtg gtcaaaagca
     3301 agactgtaat tcggctacct tgaagagtct cggcgtttcg atctgctcag agcaccgcag
     3361 ctaattagct tgttcaaaac aattgcgacg ttgatcgtct ctacaatcgc gaggcaaatt
     3421 cggcgataag ctgcgtcgta tgctcgccct gttcaagagg ggccatgtga cctgcatcgg
     3481 gcacaacaga gaatgatgca cccggaatgg ccctagccat tgcctccatt tcttctggca
     3541 gggagatcgc atcaaattgc cccacaacca ccatggtcgg acaatcgatc tgacccaaaa
     3601 gcggcgtgga gtcaggacgc tgcgccattc ctcgtgctgc cgcagccact ccctctggtg
     3661 gtgcttctag gatcatcgtg cggagtttcg tcacaatctc aggctgatga cgaaaggtcc
     3721 gctcgcaaca caggcgagga agcatttggt cggcgaggaa ggcggttccc tcggtgataa
     3781 ctcgttccgc cgtacgcaac cgattttctc gagcttcttg ggagtctgac gcagctcgcg
     3841 tgtcgcacag aatgagtccg cgaagccgct gtcgatgacg tcgcacaaat tggaaagcta
     3901 cgtagcctcc catcgacaga ccgcaaagca caaccggttc atcaatggcc agggcgtcga
     3961 gcattgccgc gaggtcatcc gccatctgtt ccattgtgac cacacccggc gtcaccgtac
     4021 ttcggccaaa tccccgcaag tccggagcga gcacccgaaa gcgtgtggat aagcccttga
     4081 tttgggcctc ccaaacccga tggtcgaaag gaaaagcgtg gaccaagaga atcgtccgac
     4141 cttggccttg gtcttccacg gccatggaaa acgtgttccc ttcggaaagt ttgtccggca
     4201 agcttacgat tcgcatagtt gtttacccct aaagagggtg aacccatgca ctcactgctt
     4261 ggggcacgca atcaaaaacg gtccctctag accgcacgtc tggaccacac gatgggcagt
     4321 ttacctttga cgggcagagt atttagcccg cattgttatt ttatggcccg gtctttcgat
     4381 aacataaatc attacaaaag atcgggcgca acatagcaga ttaacgtacg gccgggaaca
     4441 ttgagggtcc aatcaacagg cggcggcgga ccgtcgagat cgggatagat gtcgttgggc
     4501 gacatgcggc tggtgtcgat gaacaagcgc cacgctagac cccgcgccat ggccggaata
     4561 acaaatcgcg tgggttccgg agctgcgtgg aacatcagca agatatgatg gttggggcga
     4621 ttgggatctt cgcggtgggg gaacgccgct aggaaacatg tgaggcttcg tcccgcattg
     4681 gtccaatcga caggcccgcc tgcggggtta aaccaactga catcgggaaa tcccccgggg
     4741 cggacaggaa gaccggtgag gaagtccttg cgtcgcaccg ttggttcccg cctgcggaag
     4801 gcgatcaagg cctgaacaaa ccgccacaat tcccggtggc gatggaccaa tttccaattg
     4861 aaccaggaaa tagaattgtc ctggcaatag gcgttgttgt tgccccgttg ggtacgtcgg
     4921 cactcatcgc caaagagaat catgggcacc ccttggctta ataacaatgt agccatcata
     4981 ttcttgattt gccgcaatcg gatcaaattg atccctttgt gccaagtggg accttcgacg
     5041 ccataattgt aactgttatt gttgttttcg ccgtctcggt tatcctcacc attctcctcg
     5101 ttgtgtttgt ggttatagct gaccagatca ttgagcgtaa aaccatcgtg ggaagtaata
     5161 aaattgatgc tgtgatacgg atagcggcca cctggctgat acaaatcgct ggaacccgcg
     5221 atccgtgtcg cgaacgccgg aaccatcccg gggtcacctc gccagaatcg ccgaacgtcg
     5281 tcccgatagc ggccgttcca ttctgcccat cggcaatggg caaacgtatc gggccagcag
     5341 agggatgcga acgtccccac ctgatacgcc cccgcagcat cccaggcttc ggcaataatc
     5401 ttggtgtcgg ccagtagcgg atcctcggcg attgtctcaa ccacaggagg attggggaca
     5461 agattccccg cccggtcgcg gctgaggatt gatgccaggt cgaaacggaa accatcaatg
     5521 tgataattgt ggacccaata acgcaagcag tggaagatca tctcgcggac gatggggtgg
     5581 tttccgttaa gcgtgtttcc gcaaccggtg tagtttttgt aacggctgcc gccattttct
     5641 agcatgtaat agacgcggtt ctccaatcct ttgaagctca gtgtcggccc atattcattg
     5701 ccctcggccg tgtgattgaa cacgacatcc aggatcacct caataccggc cttatggagt
     5761 tcgcggacca tctccttaaa ttcgcgaact tgggcccctg gttctttact gaccgcgtat
     5821 ccgcgatgag gcgcgaagaa cgccagcggg tcgtagcccc agtaattggg ccgcttaggt
     5881 ttttgcccgt agcagtcgag gatcagaaac tcgtggaccg gcatcagctc gaccgctgtg
     5941 atgccgagtg acttcaagta ggggattttc tcgatcaccc ccagatatgt ccccggaaac
     6001 tctgccccgc tcgtaggaga tcgtgtgaac cctttgacgt gcatttcgta aatgatggtc
     6061 tccgacagat cgcgccggat gtgccgcgta ccctgccagt caaatgtgtc atcgacgaca
     6121 acgcactttg gaggacggat aatgccatcg gtggccggga gaaaatcacc cgccagggcc
     6181 ttggcgtacg ggtcgatcaa tcgtgccttg gggtcaaaac ggtgtccaat ttcgggagca
     6241 taagggccgt ctgcttggat gtggtaaagt tgcccagcct tcaatcccgg cacaaaaaca
     6301 ttccacacgt caccccagcg gtggtctgcc gggttgagat gaataatatc gctgggttca
     6361 cggtcgttga catcgttata taaaaggatc cgcattgcgg ttgcagatcg actaaaaata
     6421 acaaactgga cgccgtcttc atgcagaata gcgccataag gcaagtgatg ggcaaattgt
     6481 aggggtaatg gtcgggctaa catggacagc tgttgcgcag gttgtcccac aacaaggtca
     6541 aagcttccag cgtaccactg atgcaagtcg gttgccatgg ttcacccact attcttgata
     6601 ttctaggcca aaaaacccga accggaaaaa caaatcgccc aactttaacc gtccgtggtg
     6661 ggagaagcgc tatttcgctc acctatagcc gccatccttg gggatcggcc taacaacgac
     6721 cacttagccc cttcgtaatc cccaacatcg ggcggcatct tcgggagcgg cccgcgatcc
     6781 gtcttgggtg ggaacgatga acgaaagtgc cgcgcttggg cgcgaccctt ttccttcctt
     6841 atcgaaatcg gaccccgccc ggcctggcaa cagtgttcct caggctggga ttcctttcgg
     6901 cgcaacctga agactttcct cacaccgtcc ttgtcatgag agaataggca aactctgtgg
     6961 atttaaacga tgcgcgattg aaaaagattg aggataaggc cgacggtacg gatcaatgcc
     7021 gctggttgta tcatgccatc caaacggaat gtgactgcca ggaaggacgc ttgtcattgg
     7081 cgagatggtt cccgacaggt tgggaaaggt aattgaaagg tgagcggttt ggtcgtcagg
     7141 tcgctccaaa agaatcggaa gattgtcggg acgatacaaa tcactctctg gtcccggttg
     7201 agtagcggac catgtggaac tattatacta tgttgtgtag tttatcttgt tggttatgac
     7261 agagcgttta tttaaggcca tcgatttgat aagaacggta caaaccgtga acagctttgt
     7321 gggctaatag aagggcggga aaagaccaat tgagggtggt gttttatgtg gatccttgcc
     7381 tgagtcgtag aagtagagga gacatgccgt gttagccaag aaagtgaata caccgcagat
     7441 gggatcgctg ggtcgcggac gcaaacgttg tccctcatgc aatgccattg ttgccgcacg
     7501 ggccgtgaag tgtcgcttct gcgagtacaa cttcttggac aagcctcgca ggggtaaaac
     7561 ggcccgaggg acggtggctg acccggcctc tgtcgagaag ctggtcgcgt tcattaacgc
     7621 ccacggcgga ttggaaaacg caaaaaaggt tattagtgat gtgcgcggcc tggtagggta
     7681 tctgggttcc ttggatttga tcgctcaaca gttgaacgtt gtcggtctga taaagcagca
     7741 agtgaaggaa tagtcggcta atcctcttat gcggagctat gattcccttt atgatctctc
     7801 tggatgatgg cgatttgaca gtgcaggagt tgagcaactg cacggcgctc gcctagccag
     7861 agagaccatt gacccttgag ggaatcaacc ccagtgagga tacccacggg tgtgttgacg
     7921 cggtcaggtc atggtggctc gaatgggaca caagaatcaa gtgaatgcta tacgagcaac
     7981 aatctgattc caaaataacg cgcgacagca taacctattc accatgccgt cccatcaatg
     8041 ttgttttctg aggatggtcg tttatggctg ttctttccca gcaacctcaa aatggctgag
     8101 gatatgccgc tttaacttgt taagattctc tccaagtcgc ccgtgcttgg gctgaatctt
     8161 cggctcgatt tgggccacga tctcacgaag cggggcagcc ttggttccta ggttatccac
     8221 ggcgttcagt gctagcatca taagataggg ccctttcccc tcgtaatcga gataggtccg
     8281 tagcacagat agggcaagct cgacatcttg ttgacttcca taccggccca gtgtccacgc
     8341 agcaacaatc tggacgctcg gcgccggatc ttccagggct ttgacaaggc gatcatggac
     8401 cgccgaaaag accttttctc ccctcgccaa cagaccggtg acggcccaat atcgcaccgc
     8461 gctatcgggg tcctgtaacc cggcgagcaa ttgttcgaga tggtcctctt ccaagtctga
     8521 ggcgacttct gccatggtga ggatacgttc tagcggataa tcggcacttt gaccgtattc
     8581 gtagggcgtc ttgctgccgg cccgagcgtg gatttcgtcc tcaggaagga atcccaaatc
     8641 gcgcgtggtc aggatatgat cccgcaggac ctggcggagc tcctcaagct ttgccttgta
     8701 agccgggttg ttggccacgt tatgaacttc gtccggatcg gtggccaggt catacagttc
     8761 ctccttggcc ttgggaattg tccaaaagta ggactgttcg ggcgacagtt ttccttcgtc
     8821 gaaaagttgc ttccacacgc gtgtcgtagg ggtttgaaag agatactcga catgctgacc
     8881 ccaaggaagg tgcggcatgt aattgcggat atagacaaag cgaccatcgg tcacgctgcg
     8941 gacgagatca tatcgttcgt ccatccgacc gcggaggcca aacatataac gcggcggctc
     9001 cgtggcaaag gtcccaagga acgcccgccc ctgcattgag gccggcggct tgatcccgac
     9061 gagactgagc aacgtgggcc ccaagtccac gaaagacacc aatcgcgacg accggccgcc
     9121 gggttgatag tccggaggtg ccaaatgccg aaacttggga ggaatataca cgataagccc
     9181 gacttgaagg ccagagttat acgggcagcg tttgcaacgg ggcagaccag ggccgtggtc
     9241 accgtagaag aacacgatgg tatcgtcggc caggcctgcg tcttcaagct ccttgagctt
     9301 ctctcccgcc atgcgatcca ttgtcgtgat gttatcatag tactgggccc aatcctggcg
     9361 gacctcaatc gtatcggggt gataagccgg gactcgaacc ttcgccggat cgtggaccca
     9421 ctcgtgaggc cgacgacgca cttggctttc gtgtgtgacc gtaaagttaa aaatggcaaa
     9481 aaaaggctgg ccgggttggc ggttcttcca gtgcgccttg ccggacgagt catcccagac
     9541 ttggccaggt ttctcgaggt tgtaatcttc cttactgtta ttggtgcagt agtaaccggc
     9601 gtcgcgcaag aactgaggat aaaaccttaa gccaggggcg gtgggcacca ggctccgcat
     9661 gtgttctgaa cctgttgtgc tgggatacat ccccgagatg atggccgtgc gggccggcgc
     9721 acacacaggg gcgttggacc aaaccctcgt gtaaatcgtg cctttagccg ctagacgatc
     9781 gagatttggg gtgttggcgt aacgatcccc gtaacagccg aggtgcgggc ccatatcctc
     9841 gcaggtgagc caaaggatgt ttgggcgagc ttgttcagcc cagatggtgc ggtgggacgc
     9901 caaagtcaga ccgaaccaaa gagcaaggat cgctcccaga gaaccaaaca catgccgata
     9961 catcttgtga cctccccaaa aaggacgttc acgaggctaa atcactttgt tgctcattta
    10021 gtgtgaacac cgacccgaaa aaatgcaaca cccagcgctc gtcgtctaat gactgcggcg
    10081 atttttgcga ccagtaatgc agttgtccgc gaaatcggta agcacttggg gcagtggagc
    10141 gacgggcctt accgtggtat tatgtggaag cgatgtgtcc ctcctggaga attggaagtt
    10201 ttgggattgt ctcggcttga caggttccgg acgaaatgat atgtttggat tgggtatgcc
    10261 ttcacgcaga atttttatga ttcgtaactt catcattggc ggattggttt tcgtgacgag
    10321 ctttgtgggc atcgcttatc tttcggcggc cgagcattct ggcgaatacg atgtcgttgt
    10381 gtatggtggg acatcggccg gagtcatggc ggccgtcgag tcgcggctgg ctggccggac
    10441 ggtggctatc atctgtccgg aaaagcatct cggcggcatg tctagcagtg gcctgggttg
    10501 gaccgacacg ggcaacaagg cggttattgg tggtttagcg cgggagtttt accatcgcat
    10561 ttggaaacat taccaacggc cggatgcctg gaaatggcaa aaacccgagg aatacggaaa
    10621 tcgggggcag gggacgccgg cgatcgatgg cgagcggcgg accatgtgga ttttcgaacc
    10681 ccacgtggcc gaaggcgtgt ttgaggaatt cattcgggag tatcagattc caactttccg
    10741 ggacgaatgg ctggatcgag agcatggcgt gatcaaggag ggcagccgca tccgcagtat
    10801 tcggatgctc agtggaaaga cgtttgtggc aaaggtcttc atcgatgcca cgtacgaagg
    10861 tgacctgatg gcggccgccg ggtgtcagta tcgcgtgggg cgggaatcgc aggccgaata
    10921 cggcgagaag tgggccggag tacaaatcgg tgttcttcac caccgtcatc atttcggagt
    10981 gctaaaaaag cctatcagtc cctactggat accgggtgat cctagcagtg gggttctacc
    11041 ccgaatcagc acggaaccgc ccggcgaata cggtcagggc gatcatcgtg tccaggcata
    11101 ttgtttccga ttatgcctga cggatgtgcc ggaaaatcgt gtcccattcc ctaaacccga
    11161 gggctacgat cctaaacagt acgaattatt gttgcggatt ttcgaggccg ggtggcgaga
    11221 gaccttccat aagttcgatc ctatcccgaa ccgcaagacg gatacgaata accatgggcc
    11281 aatgagcacc gataacattg gttacaatta cgactatccc gaagcaacct atgagcggcg
    11341 acgggagatt atcaaagagc acgagacata tcagaaaggc tggttctact ttatcgccaa
    11401 cgatccgcgg gtgccccgag acgttcaaga ggagatgaac aggtggggac ttgcgaagga
    11461 cgaatttgtg gacaatggcc actggcccta tcagctttac attcgggaag ctcgccgaat
    11521 ggtgggcatg tacgtgatga cggaacacga cctgctaggc aaaaagacga cacccgactc
    11581 ggtgggaatg ggttcttaca ccatcgatag ccacaacgtg cagcggtaca tcacgccgga
    11641 gggctacgtc cagaacgagg gcgacattgg tgtgcctact cccaaaccgt atgccattgc
    11701 ctacggctcg cttgtcccca agcgggaaga ctgtgagaat ctgctcgtgc ccgtgtgcgt
    11761 ctcggctaca catgtggctt atggatcaat ccgcatggag cccgtgttta tgatccttgg
    11821 tcagtcggcc gcagccgcag cggacatcgc cattaaacag ggcgtcgccg ttcaggatgt
    11881 gccgtatgcc gcgttgcgag aacgactgct caacgaaggg caggtactag aatacggcgg
    11941 caaatgatgg catcagacat aggtcgtacg gtgaggaacg taataggatc attggtttta
    12001 tagtcagttc ggttgcgagc aacttagaaa gtcggcattg cagaggagag agtcgccgaa
    12061 atgaaaaaac gtaacgtttt tctcgttagt gtgggttatc tgatggtact cgccgcaatt
    12121 tgtgggggag ccgttggggc cgagtcagca caaccacgtc ggctgacaat cgttcccttc
    12181 acacgggtcc atgcggaaga cacgttttgg gcggtacgca ttaagaccaa tcgagagcga
    12241 tccatccccc acaacttgaa atggtgcgag gatacggggc ggatttccaa ttttgccaag
    12301 gccgctggtt tgatggaggg taagtttgaa ggaatctatt tcaacgactc tgatgtttac
    12361 aaagtcctgg aaggggcttc ctatgccctg gctcaacaat ttgacgccga tttggccagc
    12421 caggttgatc gcattatcgg acttatcgcc gcggcacagc gcccggatgg gtaccttaac
    12481 acgtattaca ccttaactaa tctcaatgac cgatggacgg accttcccgt aaaacacgaa
    12541 ctatactgcg ccggacacct ttttgaagcg gccgtggcac actaccgcgc cacaggcaaa
    12601 cgcacgtttc tcgatgtcgc catcaaactg gccgatcata tcgacagtat ttttggagag
    12661 ggcaagaggt acggtgttcc gggacatgaa gagattgagt tggccctcgt caagctttac
    12721 gaggtaacag gagaaaaacg ttatttcgat ctggcgaaat ttttcatcga cgaacgcggt
    12781 aacacgaccc atcggccgtc gtatggccct tacagccagg accatctgcc cgtgcggcag
    12841 cagagcgaaa ttgtaggaca tgccgtgcgg gccatgtatt tgtactcggg cgtcgccgat
    12901 gtcgcggcct acacgggaga tcccgaactg atcgctgcta tggaccgcat ttggaaggat
    12961 gtcacgctgc gcaaaatgta tatcacgggc ggaattggtg cccgccatga aggcgaggcc
    13021 tttggcgagc cgtatgagct tcccaatgag tcggcttact gcgagacatg tgccgcgatt
    13081 gggctggtct tttgggccta tcggttaggg ttgatgcatg gcgacgcgca atacgccgat
    13141 gtcctagaga gggcacttta taacggcgtt ctttccggtg tggcattgga tggggaacat
    13201 ttcttctacg tcaatccact tgcttcgggg ggaaaccatc atcggcaacc gtttttcggt
    13261 tgtgcctgtt gcccaacgaa tgtagtaagg cttatcccct ccgtaccggg ttatgtgtat
    13321 gctgtgcggc caccgaccgg ggaaaagcct gcggaattgt tcgttaacct ttacttgggc
    13381 agtacggccg aggtagagct tgaagaggat aacgtggtaa aagttaccca ggagacgcgg
    13441 tatccttggg agggccgcat tgtcctgacc gttactcccg aattggagag ggaatggtcc
    13501 ttgcggctgc gcgtgccagg atggtgccgc gaagcccgcc tggctgtcaa tagtgagggg
    13561 ccgacgcctg ttgacttgac gaagcttgac cgcggctatc acctcattcg cagggtgtgg
    13621 aagaaggggg ataaggtgac gttagacttg gcgatgccgg tggaacggat cgaggcccat
    13681 cccaaggtag ttgccgatcg cggtcgggtg gccattcagc ggggaccatt ggtgtattgt
    13741 ctggagactg ttgataacgg attcgacgtt cgcacggcca tcttggccaa agacccacag
    13801 tttgagacgg aatttcggcc ggacttgctg ggtgggattg tggtgattag ggccacggct
    13861 gccgacgggc gacgtttgac ggcagtgcct tatcatttgt gggatcatcg tgatcctggg
    13921 ccgatggtcg tttggattcg gcaggcaggt aaatctcggt atcccgatcc agaggaccct
    13981 gcttgggagg gaaggcttta tcgtcccgta gatccgacca gcttgactgc cgaggactcc
    14041 ccgacagtca tggaggcagc gcgcccttcc gcgtcccatt gttggtctta tgattccgtg
    14101 gaagctctca acgacggcat tgaacctcgg aactcgaacg accacagtat cccgcgattc
    14161 acctggtggg atcaccgggg gacgcaggag tgggtcgaat atgaatggga caagcccgtg
    14221 gcgcttcagg gtgtgcgggt atactggttt gacgatgaac cgcgaggagg gcattgccgt
    14281 ctcccggcct cgtggcggat tctctatcgc aaaggtgatg catgggtccc ggtgacaagc
    14341 caatccgagt tgccaattgt caaagatggg tggagcgaag cgagctttgg tccggtccaa
    14401 acccagggac tgcgtttaga ggttcaactt cggccaaact ggtctggtgg catcttggag
    14461 tggcaggtaa ttgaggcaaa gtgaccgggc gggggttggg gacgagcctc aggtctcgac
    14521 ggctggcaat acgtgctgaa tgccggatct gcgctcacta ggatcgcctc tctcgccgtt
    14581 ttgttaggaa gggctaccgg aaattgtggc ccgcgctggc cggtgccatg ccataatcag
    14641 atatgacacg ccgtgtctga gttaggattt caatctgttt gcggccggtc aggtgattgg
    14701 ccaagcgcct tgggtgtcac gatttttgct cgccggggtg gtgtcatacc gtggcccaga
    14761 tagaacccag ggtgcgctgg ttgattgtag cccacgtttt gccacgcgat tcccagccga
    14821 taaacaggat cgtgcatcaa tgttaccaaa cgatgctcgc ttggcagggg tgttgtgaaa
    14881 atgtgaagac tccggttatc agcggagcgc caaatgacct cttcacgcca atcgccaaag
    14941 atgtcggcac acagagtggg atttgctttg gagccattat tccaggtgca cccatatcgg
    15001 gccgcgtcga acaagacatc gcatgcttcg cgctcgggaa tccacttctg aatctgggtt
    15061 cggtcaagca cttcgcggag caggtcccca tcccaccaga cgcccatatt gcaggacgcc
    15121 ggcgcgcggt tggcgatctg ttccccccga cagttgaaaa gccctctcaa gccgggcccc
    15181 actgcccaac actccaaacc gggatgacgg ggatcaatgt ccagggccaa gccgcgacta
    15241 acatctcggg ctgcaagccc ccatataatc tcccccgttg ccgcgtctcg gaggttggcc
    15301 ccaaagggat gtgacggacg ctcgtgaatg gaaaagattt ccagccctgg ccgatgcggg
    15361 tcgatgtccg tcacgtgcat cgcgtccccg tggcccaact cggtgctgta taagccctgg
    15421 ccatccgggc caatgacaca ggccccgtaa atgatttcgt cgcgaccatc gccgttgacg
    15481 tccgccacag aaaggttgtg attgccctgc ccacggtact tgcggttttc ccgcggatct
    15541 gtgtcgctgt cgaaaaccca cacaagggag agttggccat ctcggtagtt ccaagcggcc
    15601 aacaccgttc gggtgtaata gcctcgacac atgactacgc taggttgacg gccatccaaa
    15661 taggccacac aagtgagaaa acggtcggag cgattgccgt aatcgtctcc ccatgcgcgg
    15721 acatcgcccc gcggcgggac atacggcacc gaaacaacgg ccttgccggt gtgaccttca
    15781 aaaacagtca agtattcagg ccctgtgaga acaagaccgt ctgcattgcg atgattggct
    15841 gaaggtgagc cgatcacttg gcccgtacca tcgaccgtgc catcggaggt gcggcaaacg
    15901 acctcggcgc gcccgtcgcc gtcgaagtcg tacacaagga acggcgtata atgggcccct
    15961 tcccggatgt tgggaccaag attgatccgc caaagaaact taccgtcaaa ggtgtaagct
    16021 tccaggagcg tctggcctgt gggcccgcgt tgcgagttat cgcgaggccg cttctcctgt
    16081 ttgaggacaa gctcatagcg gccatcgccg ttcaaatctc ccacactggc atcgttgggt
    16141 gtgtagcctg gtaaggtctg caaaggaatg acccaatggg tttggccttt cgcgacctcg
    16201 acggttgccg tctcgcccgt ctctttgcct gagataacat ttcgcacttg gtagaatcga
    16261 ggccaggggc tatcacgatc ttcatcgacg aaattggttc gatccaaaag cggagaatcg
    16321 gtcaggcgaa tccatgtgcc atcttcctgg cggcgataca cgtggaaggc caaatcatcg
    16381 gggtcgtcgg caagcaaccg ccagcttacc attaccgtcc catcaccgcg gtcgatggcg
    16441 accagacccc gatcaaggtc ttccatctga cgttgggtaa aaacctgccg ggtgactccg
    16501 aggaaaacaa gaagggcggc aagagatcgt aacataaaat ggatcgctgc tggcaagact
    16561 gagcggtgct tatggttcag attgacaatc atcgttagca aaaacgtgga ccggtgcgaa
    16621 aggtttcttc aaaaccagcg agatatcgcc ttttgttatc cctctattgg gtggttaatt
    16681 tgtgctattt gctcaaacga attggcaaga atcgaaagtt cgacaatttc cgtttcaagg
    16741 agcaagcggt aaactaaata cgagaggatt ttgcttgcaa aggtcatggt atttcctccc
    16801 cgggagaatg ggaagagacc cgttcgacgt gaaaggccgt ggccttttat tgtgatggca
    16861 acaatttggc aaggttatgt tagggcttga ccgggagact ttttgaacaa ggggtggaga
    16921 tgccggccat gatcgaattt ctttgtccga acggccatcg tattcgttgc tcggaagatc
    16981 gggcagggaa accggccaag tgtcccaagt gcagtgtgaa gtttctgatt cccacgctgg
    17041 aggagattcg ggcagctcaa ggcgaagacg ccggttcgaa cgccacgttt tcacgagatc
    17101 gtacccgcat tcagttctta tgtcccaacg ggcatcggct ctttggatca gcagatcttc
    17161 aggggcgtcc cggccagtgt cccgagtgtg gtgtccggtt ccgcattcct gtgatcgagg
    17221 agctgaccga cgacgatgca gacgaggcgg tgccgctcag tgtggctccg gagccttcgg
    17281 caggtcccca actggcgaca gggactgggt taggggactc ggccatcgtt ccggagagtg
    17341 gcatccactt ggagttgttt tcgccacgat cggccggcga tagtcgaacg cccgagagtt
    17401 ctatctcatc gccgacatcg attaagggca gccatttagc acggatattt gagcggcttt
    17461 ggaacggtcg cgcagcgggt agcgtggtca aggtccaatt agtagatggc caggccttta
    17521 cggtaacgaa gtacagcgct tcttcgaacg atcacgaata tgcggctttc gttgtggagg
    17581 gttcagcttt tgggggtggc ttggtcgttg ttccttggca aagcgtgcgc atgatttacg
    17641 tcagcggcgt caaagatatt ccatcccaat tgcttgactg atttggcatg tgggccttcg
    17701 agagtaggac ccattcgggt tgatgagggc gaaaaatcgc ttgtccatgc aactgccaac
    17761 tttgtgatga aaactttctg gcatattgag ctgatttcgt cgcctgtgcc cgagatccat
    17821 ttagaactta tcccaaagag tccccctctc cctccggaag agggttgggg tgagggcaag
    17881 cgaatgtcgg aatcgattga gaaaaccggc agcgattcga ggcaccttct cccccgagcc
    17941 ctctcccgcc gaacgcgtta ggggagatta tgggataggc tttttagctg gcgcgtcttt
    18001 gccctgcgcg tggaatttcg aggggttttt agtcaaaatg gggagaggta tacggttgat
    18061 aaggacggta atggacgtat gggcaggggg ttgaagattg ccgtgagatt aggccggggg
    18121 cactgcgtcg tagaacgggg taactgtgtg cacgttaaac attcctgcgc tttgaaaaga
    18181 gacgacgatt gccctgagat ggaatgatgt ccgagttgat acgggtttct cacccaacag
    18241 cactttcgcc cgccgtaacg gcggcgatga tgacgccaac gcctatccaa cgggagacgt
    18301 tgcatgggcc accgaagccg attgacgttt tccacgcggt acgacggcgg tggtggctga
    18361 ttcttttgct ggggacgatt ggagctgtgg ccgtcatgat tttcgcctgg cgcaccattc
    18421 ccgtccagta cacagccacg gcatggttgc aagtctcctt gcagcgtccg cggattattt
    18481 ttgacacgcg cgaggatgat cccctcctta ggaatcgtca aacgcaagcc accttagtca
    18541 cgagcaaccg agtcttgggg gctgccctgc gtcagccggg gattagccaa cttccgtata
    18601 tccgcaacca aattgatccc atgatgtggc tacggcaaaa gatccgcgtg cgatttgccg
    18661 atcaggcgga gatcctggag atcagtatga cgggagagga ccccgagcag atcgcagcca
    18721 tcgtcaacgc ggtcaaggac gcctacatgg ccgaggtggg ggacgtcaac cgaacatacg
    18781 ccctggagcg caaacgcctg ttggagcaaa actacgccca aacgttggag ttggtcaaaa
    18841 agcgggacct ccgttatcgt gaattggcca acgcagtggg gagtgccgat tctgaagtcg
    18901 cccggcggaa ggcgatcacg gcgctggaaa cgctggcgga ccatcggcgg cgcgtgaatg
    18961 agattcgaca gcaactggga ggtttggatc gcaagatcac gctcttacag acacggttaa
    19021 agtacctgga agagcaccgt ccgcaaagct cggaagaaga acgccgtgag cgcgatgagg
    19081 cggtcattcg gcagattgtg gaggccggcc tccgcgcgga tccttggatt tcctcggccc
    19141 aggcgaggct atcggcattg gaggcccgct ggtttgagga acgacagagg gtcgttcaat
    19201 gggagaagtc accgacgatt ctccgcctcc aagaggagat gcaagccctc cgcgaagcaa
    19261 ttgagaaccg tcgcaacgaa ttaatcccgg ggttaaccca atacgcgcgg gagcagttag
    19321 agaaagggcg ggcaaaagcc gcccccacgg aggccgaact tatccaggcc caaattgcgg
    19381 aagctgaatt agaacgggct gtgctcgagg atgaactaaa agtggccctg gaacagtttc
    19441 agaaggaggc tgccgaggcc gaacaacttg gcaaatactc ggccgatctg gaggccgaaa
    19501 aagcggaggt ggatcgtctc aaggccgtgg cggcaaaaat gggagatgag ttggagcgat
    19561 ggaacgtgga gttggcggcg ccgcctcggg ttcgtgtgtt ggaagaggca agcacgcctc
    19621 gggtcagcaa tatcaatgac aagtaccgcc tggtaacctt cctggggttg atgacatttt
    19681 tgggaacagc tgccgtcgta gttttgctag attttcttct ccgccctgtt agctccgcct
    19741 atcaaatcag ctacggtctc ggtttgcctg tgctgggcga tctgccactc attcagcgcg
    19801 gtcctttctc ccgcatcact tcaaatggcc aaatgccgga gcatgttaag acgatcttgt
    19861 tggagtccgt tgatcagctt cgcacggtgc tcctccatcg ggcaaaacgg gatggtgtga
    19921 agtcggtctt ggtgacaagt gccttggctc gggagggcaa gacgaccctt gccagtcagt
    19981 tggcggttag catggctcgg gcggggcgac gcgttatctt ggtggatggt gacctacggc
    20041 ggccacgttt gcatcgtgtg cttcggcgag atctgcatcc cggactggcg gaattggccc
    20101 gaaatgaggc gacgccccaa gagattgtcc aggcggctga acttgagggg ctttacttgg
    20161 tgacggctgg gcattgcgat gctgcggccc tcgaggcggt ctcccaggga aggctcgatg
    20221 gctttctcga ttccctccgc gacacatttg atatggtttt gatcgattcc agtccgctcc
    20281 tgacaaccgc ggattccatg attctggccc acctggcaga tggggttgtc ctttccgtgg
    20341 tccaagaccg aactcgcttg ccacggctct tcgaggccgt ccaacgattg cgggccgtcg
    20401 atctgcccct aatcgggatt gtaatccatg gcacggcgcc cagtcggtac cgcagttatt
    20461 accggtcgta cacaattgat gtagaggttc gaaagtcgtg agccgacttt cagctcatgg
    20521 gtcaaacaat gaacgggatg tccgggacgg tatcgcggag tttcttggaa gagttaggct
    20581 ggttggggag gttcatcatg aagaacagtt ggattgttgt tctgggactg acgctctttt
    20641 tgagcaggac agttttcggc caggcgcagc cccaaccttt gaacgtcgtg tttttcctgg
    20701 tagatgacct aggttggacg gatgtgggct gtttcgggag cacattttac gaaacgccca
    20761 acattgatcg gctggcggcg gaagggatgc gatttttgga cgcttacgcg gcctgccagg
    20821 tatgttcccc gacaagagcc gctattcaga ccggtaaatg gccacaacgg acggccatta
    20881 ccgattacat aggggccccc caaccagcgg gctggaaccg aaacaccagg cttcttccgg
    20941 cagcctatga aacggctctt ccgcttgcgg agatcacgct agccgagttg gtcaaacggc
    21001 ggaattatgc gacgttcttt gccggaaaat ggcacctggg gccggaaggg ttttggccgg
    21061 aaaaccaggg gtatgacatc aacatgggcg ggattgaccg cggcgggcct tacggcggca
    21121 acaagtactt ttctccttat ggaaatcccc gcctgcccga tggaccgccc ggcgagcatc
    21181 ttccggatcg attggcgaga gagacggcca agttcatcga agagcataag gaccaaccgt
    21241 ttttggcgtt tttgagtttc tattctgtcc atacgccatt gatggcccgg gaggacttac
    21301 ggcaaaagta tgaggagaaa cgccgtcggc tgggactcga ggcgaaattc ggtcgagaag
    21361 ggccgcggga tgtccgcctt gtccaagaac atgccgttta cgcggggatg gtcgaggcga
    21421 tggaccaagc ggtagggaag gtcctccaaa aaattgacga attgggcctg agagatcgta
    21481 cggtggtgtt tttcaccagc gacaacggag ggctttcgac cagcgaggga tggcccacgt
    21541 cgaatcttcc cctacgggcc gggaagggct ggatttacga aggcggcgtg cgggttcctt
    21601 tgattgtgcg ttggcccggt gttgttccgc ctggcacggt cagtcgtgtc cctgtgtgta
    21661 gtgtggattt ctttcccacg attggcgatc tggccggtgc accatggaat gatatccctg
    21721 tggatgggca gagtcttgtg cccttgctga agggaaagcc gtttcccgaa gatcgtccgt
    21781 tgttctggca ttacccgcat tatggaaacc aaggtggggc gcccagcgcg gccgtgagaa
    21841 aaggcccctg gaagctgatt cattggtatg aagatgattc ctgggaactc tacgatgtcg
    21901 ttgccgatat tggcgagaca caaaacctcg ctcaaaagca ccctgagcgt gtggctgaac
    21961 tccgtcagct tttggagacg tggttgaagg atgttggggc aaggtttccc accgccaatc
    22021 ccaactatga tccaaacaaa ccggatggga ggatcgcggt ccggccgcag taaaaagtgg
    22081 gagcggtgcg cgtttggcag gaaatgtggc ttgtgaacga ttcgcttcgt aaattattgc
    22141 cggcgtggtt ggcaaagaac ggctggttct tcctggccgt gggggtgatc atcgcggcag
    22201 cgatcgtcga aggccactgg tcattgcgtt ggggttccca cagcgaacgg gtaatggcgc
    22261 cattggcgca ggctgtcatg caaacgccgc tgacggtggg agaatgggtt accgacgaag
    22321 cggtgcagcc ggacccccgc gtgctcgaga tcagtggcgc ggaagccttg tttcaggcca
    22381 cctatcgcga atccacccag ggtgatgttg tgacggtgta ccttttagcc gggtggtcgc
    22441 gagacatttc catccacacg cccgacgctt gttatccagg ggcgggcttt gtgatggaga
    22501 cgtcccctca accattcgct ttctcctatg aagcgaattc ccaggcaggc gagcagtcct
    22561 ccacgggcct tcggcaggcc gagttttcaa cggccatctt caccaagaca gaaccaaccg
    22621 gtgtgacgcg gctgcgggtg ttttggtcgt ggaatgatgg gcgcggctgg caggcccccc
    22681 gatttccccg ttgggctttt ggaggccgac ggcctttggt caagctctat ttagtgagcg
    22741 agtctccgcc agggcaaccc acgcacgaaa gtcccgccct gcgtcttgcc tcggccctgt
    22801 tgcctgttct cgatcgacaa ttagaaacgg cattatcaac ccgccccaaa gaacggatgt
    22861 cagtgcctta aaaaatggcg ccagctaaat aggttaagag ttgtcgccag cataatctgc
    22921 cccggtcttt tcagaaatcg aaccggcgct tacccgacgc cgtctctagt tcgatccaat
    22981 cgacctcggc ggtgttggcc cctgtgggga ggtaaatccg caggatgccg atgggttttt
    23041 cccagggaac gcgaacggtg accgttgtca tctctgtgcc tgtgacactg taggggacgg
    23101 actgggcggc gtcctgactt gctcctggtg gaatccactc aatttttccc tcaccggctt
    23161 gagggacctt aattcggaaa gttacctgga ccggtccggg ctgcttgccg acaccgaatc
    23221 ccaagaatgg cgtggccgtt tgggcctgca acttaagcag gccggcgtcg atcgtcgcct
    23281 cgcacccacg gtttttccaa cccattagcg gatcagaggt ctgtggcccg cgatagtcgg
    23341 gattgacctg cggaatgaca gcgtcggtat cctggagaaa gccctccagt tctttggcta
    23401 acctgccgac aatctcggga tgttccgctg caacatcgtg agtttccccg atgtcctcac
    23461 gaagattgta aagttcaaaa cgatctgtgc cgtcaggatt cccggcgaaa aagcggatca
    23521 acttccagtc gccgtcacgc acataggctg aggggagaaa gcccggaatt tgctgggcct
    23581 ggttctctcc accgtgcgga aagtggcaaa agacccgcgt gcggacaggg cctttgccaa
    23641 gaatggccgg gacctgggaa acgccatcca ctttgtgacc cggcaaagga aggccacaca
    23701 tctctagaat tgtgggaaag aagtccacac tggaaaaaag tacgtccgtt accgagtccg
    23761 gttgcgtgtg ccctggccaa acaatgatga gcggtacccg cgtgccgcct tcgtagatgg
    23821 aggccttacc gcttctcaac ggtgtgttgc tcgttgctgg aatctccgcg taaccaggcg
    23881 gttgcgtttc gttctttggg ggataggcaa aaccaccatt gtcagagaag aagatgataa
    23941 tggttcgctc ggcaagaccg gccttgtcga tggcctcgac gatgcgtccc accgcgtcat
    24001 ccaggctttt caccatggcc gcataaaccg ggtttcgttg gggcgagtca tccttggctt
    24061 tggtcttaaa atactcgacc aactcgggtt tggccgacca aggactgtga acggagaaac
    24121 accagtagtt caggtagaaa ggcttatcct tgttttcctc gataaatcgc gcagcctctt
    24181 gagacatgcg atcctcaatg tgctctcccg gctggccttg gatggcaggg tccttgataa
    24241 acttccacgg ggcgagatag ccacctccgg gacctggcgc cgccggggta ttagggaaat
    24301 cgagcatgaa gccttgctcc tttggttcat aagggccgga atgcccaaga tgccactttc
    24361 caaaatgggc cgtcatgtat ccatgttcac ggaagacctc ggccaaggtg acgtactcgg
    24421 gtttaagccg ggttaggcta ttggctgtac ggatccggac attcggttgc ggtggaacaa
    24481 ggcctttttc cagcactgct tgtggaagat ggcaagctgg tgatgtgata ccaatccgcg
    24541 cggggtagag acccgtcaag atgctggatc gtgtgggaga acaaagcgga ttagccgcgt
    24601 aggcctgcgt gaagcgcagt ccgcgtctgg cgagacgatc gagattgggc gtctcatgat
    24661 a
//
LOCUS       DAOSUT010000053        24551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1341_length_24551_cov_9.880307, whole genome shotgun sequence.
ACCESSION   DAOSUT010000053 DAOSUT010000000
VERSION     DAOSUT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_24551_cov_9.880307"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1253)
                     /locus_tag="PLD05_06665"
     CDS             complement(84..1253)
                     /locus_tag="PLD05_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP77153.1"
                     /translation="MPREKMTSRERLLAALNHQEPDRVPIDLGGNQTGIHRLAYEALL
                     KHLGWEETIEIMDAVQQLARPSEAVLERFHVDTRYIAAKPAESWKGEIVRAERDGRVW
                     LDLVDEWGVRWSMPEDGGLYMDITYHPLAKATMKDLQEYPFPKGDDPSRFVGLREEAL
                     RIRRETPYAVVSGISGVVYETCWYLRGLERWFIDMIEQPEFCAALLDRTLRFWMDWFR
                     LFLKEVGDLVDVIMIGDDLAGQDGPLFSPSFYQAIVKPRHKALVQFIRSQTHAKIWYH
                     TCGACRSFIPDLIDNGIDILNPVQISARDMDPTTLKAEYGQQLAFWGGAIDAQHVLPS
                     ASPDEIREHVRRHLEIWKPGGGYVFNNVHNIQAGVPPENIVALFDAAYEFGFYEQ"
     gene            1544..2995
                     /locus_tag="PLD05_06670"
     CDS             1544..2995
                     /locus_tag="PLD05_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HOP77154.1"
                     /translation="MHVEFHGKTQVALASLLAAAGLTAGKVVVGILTNSLGIISEALH
                     SGLDLVATGMTLWAVRIADRPADEEHRYGHGKFENLSALAETALLLLVCVWVFYEAIQ
                     RLFFRAELEILPSPWAFGVVIASIVVDLWRAHHLREAAEKYSSQALEADALHFASDVW
                     SSLVVLLGLTCVLLAQRLDWPWLIHADALAALIVAILVSVICVRLGLRAIDALTDRTS
                     RSLAHLVEHSASQVKGVEEVRDLRVRQSGAGVFADMTVCVPRELGLAQAHEVATNVTA
                     AIRSQLPEADVVVHVEPVAREDEDLVTSIRALAASLGLAVHDVQVISTPVGNSIELHV
                     EVSPDLNLEQAHDLVTRLESTIKSDLPKIESVLTHIEPNGVTRAESEARDQAPIRTES
                     FHQAIADFSQASGTPMNGHALVTRQTPQGVSLSFHLHLDGTLPIGTAHEISRTLESYL
                     HRKFPELSRIVIHIEPHPPAEGHSPEPGPPQVS"
     gene            complement(3013..>3675)
                     /locus_tag="PLD05_06675"
     CDS             complement(3013..>3675)
                     /locus_tag="PLD05_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77155.1"
                     /translation="EGQRERRSRFRRREDRPARPEESSVKSEDGMPVISRPVRLPRRP
                     EREQPKAPAEPLSEEVLTGKEPMRSFADLMQYFAAKGVIRPSTPKTSGSHSGRSGKKN
                     KGGKDKKGGRSSEDRRTPEQAAPSPADRLGDQPKPAESPSPTDHQQREQPTSEGTSIP
                     NAPAQSQDTITGNPRNQGVESAGTIPVPDEGQVPGPDAIAPAEEAGSSEGENQQKSSD
                     GP"
     assembly_gap    3676..3775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3776..6375)
                     /locus_tag="PLD05_06680"
     CDS             complement(<3776..6375)
                     /locus_tag="PLD05_06680"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOP77156.1"
                     /translation="MATTEVTVSFHGLSRELQIPEKRIQAVVQLLDAGNTVPFITRYR
                     KDQTGGLDEQQIRTIQNRVTKLRQLAERKQAILRAIEAQNKLTPTLAKKILAANSLKR
                     LDDLYLPFRPKKQTLATIARERGLGHLAEEILNADPICADLDKRAADYVNPDKQILSG
                     ADALLGAGHILAEMFSENAALRARLRDILKKTGVIKTVRIAPEVSATPQQPSPAGQSP
                     TPSPATELPTPASESSVVQAVTTPPEEQTAAAGSSLASEPASETAATEPDQTGSIPTE
                     AAPGETEDLPLAGSREGMERPSEAASPAGETEQRLEAQASEHPNAENTETGEAAPIAE
                     SPSGPAEVQQPTDAMPPAASSGESSQQAATATSPPTNAAKTPNPRAAAKEAEKRRKEE
                     QRIKEFADYFDFQEAIRSIPPHRVLAINRGEALKIIRVKIKADEEALDRAVEEICVPK
                     DHPHADFLRGCARDALNRLILPSLEREIRRELTEKAEAHAVKVFAKNLRRLLLQRPVR
                     DRRVLAIDPGYRNGCKLAALDEYGNVLDHKLIYLVEKKGYTREMAKETVCELIDKYQL
                     TCIAIGNGSGSRLAEMFIAELLENELKNRGIGYTIVNEAGASVYSTSQIGREELPNLE
                     ASYRGAVSIGRRLLDPLSELVKIDPANLGVGMYQHDLKEKHLRESLDEVVESCVNYVG
                     VDVNTASPALLGYVSGLNKLTARRIYEYRQEHGAFRSREELKKVPGIGEATFVQAAGF
                     LKISGGDNPLDATWIHPESYELAHRVMEKLGITAEDLTKADKRAELAARINQVNVEAL
                     AAELNCGVFTLRDILAQFVRPGRDPREELPPPVFKQGILKLEDLSPGMELMGTVLNVV
                     DFGAFVDIGLP"
     gene            7108..7272
                     /locus_tag="PLD05_06685"
     CDS             7108..7272
                     /locus_tag="PLD05_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77157.1"
                     /translation="MEGISGWDIALWSVAAFAAVMLLVRVMLSFRQYWVEYYRMQAEA
                     ARKRKPKKEG"
     gene            7373..8776
                     /gene="miaB"
                     /locus_tag="PLD05_06690"
     CDS             7373..8776
                     /gene="miaB"
                     /locus_tag="PLD05_06690"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOP77158.1"
                     /translation="MGKRFYIETVGCQMNELDSELVIAALLEAGYEYVPRRQDADIIL
                     FNTCSVRQHAEDKIYSALGRLKHLKEQKPGKIIGVLGCMAQKDQGLILKRAPHVDLVV
                     GPGQVARLPELLQKLGESGSPIVEVSLDRRGVSQQRVRASFLPLDRKERPSVRVTPYQ
                     AYVRVMYGCDKFCTYCVVPRVRGPEQSRSPSEILDEVRQLADQGCLEITLLGQTVNSY
                     HWTEGGKTWRLSDVLAEVHEVEGIRRIKFVTNYPCDMTPDLLQAVRDLPKVCPYLHVP
                     AQSGSNRILQRMKRGYTVEEYREMLAAIRETIPGAAITSDFIVGFCGETEEDFAATVK
                     LVEDTRFKNSFIFKYSPRPGTKAAELWPDDVPEEVKRDRNNELLAIQNRIAEEENRRF
                     VGQVVEVLVEGPSKASGKQRQSDGSLQLTGRTACDRIVVFPGSSDLVGQFVSVKIEAV
                     SAVTLFGHVLAAEAAVS"
     gene            8838..12011
                     /gene="glnE"
                     /locus_tag="PLD05_06695"
     CDS             8838..12011
                     /gene="glnE"
                     /locus_tag="PLD05_06695"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOP77159.1"
                     /translation="MEVETLRSFLENPEGANRWLSGLRLRDPRVAHRNLLSIARQGVP
                     LDLLTVLIQQMEKVLPELADPDMALNNLERFFAATRNPLSLASLFERDPEALAPLLQI
                     FSASQFLSEILICDPESYDLLRLTEGQPVSRQALIDELTSEIAALTHENEIMATLRRF
                     KRREILRIAYGDFVCGHSLSTVTRQISYVADALLEAAYQAARKKLRGKLGEPRTVDGS
                     VARFVILAMGKLGGVELNYSSDIDLIFLYEDEGTTDGPRSVSNAEYFHRLVQEIVRFL
                     TLRTELGAVYRVDRRLSPEGEQGPAAISVPAALRYYDMRGRTWERQAYIKARPAAGNL
                     DLGYAFLEQLEPWIYRRYLTRADISGIKTLRRKIEQRARAQGDLRDVKNGSGGIRDIE
                     FVIQFLQLLHGADLPGVRTGNTLDAIAGLEQAGCLTHTESAYLQESYHFLRKLEHRLQ
                     IMFDLQTHTIPQGAEEIQKLAVRMGYHDTPTRSASEAFEADYYRFTFRNRQIINHLLH
                     DAFRDETETSPEVELVLDPEPSPDFIKQVLSRHRFSNPEQAYRNLMSLAEERVPFLST
                     RRCRHFLASIAPMLLSAIATTPDPDATLVTLDQVSDSLGGKGVLWELFSFNPPSLKLY
                     VQLCAYTPFLASLLISNPGMIDSLMDSLVLDRLPSREFMENSLRELCLAAEDIDPILH
                     AFKNDHLLQIGVRDLLGKEDVEAITRTISELAETCLMEIVVREYEHLVLRYGQPTIQE
                     GPDQGRRAEMVILALGKLGGQEMNYQSDLDLVFLYEGDGQTVPVVDSGYVRRGEVTTN
                     QHFFSELTQRIIRRASYLGPHGRLYQVDARLRPTGRSGLLVFTLNEFRQYFESGQGQL
                     WERQALCKARPVVVTPRMQPVINQLLHEVIHCQPWRPEFAHEIAAMRKRLEATAKGLD
                     IKRGCGGTVDVEFLVQMLQLRHGREFPQLHTTSTMAALRMLRECGVLEERDFKELFES
                     YRYLRTLENRMQLVAPTARSRLPDNPVELSRLSHLMGAETPPAMIRTVEAYLARNRSH
                     FERVVEKLANERIALPSSPNQETPHDN"
     gene            12067..12513
                     /gene="ybeY"
                     /locus_tag="PLD05_06700"
     CDS             12067..12513
                     /gene="ybeY"
                     /locus_tag="PLD05_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOP77160.1"
                     /translation="MYRIAISKQVKSCKINRNLIRKAVRVVLQQAGIETAEISIAIVD
                     DATIAQLHGQYLGDPTPTDVLSFVLEEGDHHLEGEIVASAETAAAWAKRLAWPCENEL
                     LLYIVHGTLHLVGFDDQTPVARRKMRREEKAVLHKLGVVPPVRSRR"
     gene            12590..13915
                     /locus_tag="PLD05_06705"
     CDS             12590..13915
                     /locus_tag="PLD05_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOP77161.1"
                     /translation="MSEDQVLLWLCVLGLGISCVASTGTRALSDFPSHDLEELCRKRN
                     RMERFREVLSRYRRVSIASELVSLLAVLVVTVAAVILGSQLWHEIKQAEPSARLVLCQ
                     RLLLEGLGGVLVILMFTVFIPRAVSLLWAARVVYYAWPFWRFLERLMTPLLFVNRVVE
                     VILARSAGVQLPQESEEQFEEEIRTLVSEGEREGLLEGEAREMIEGVIKLSDVTVSEI
                     MTPRTEMMCLSAKLSWDEMLESVINEGHSRIPVYEETRDNIIGILYVKELLPYLARSA
                     EQERPPWTSLLREPFYVPETKPVDELMQEFQRTRNHMAIVLDEYGGVSGLITLEDILE
                     EIVGEIGDELDQEVVEEIRIDDQGRAEVQGTARVEEVNRRLDLGLPESEEYDTIAGFL
                     MTHLGRVPHVGESIVFGKFRIKVLDGDRRRIRRVIIEPAEPVALPEAQN"
     gene            13987..14994
                     /locus_tag="PLD05_06710"
     CDS             13987..14994
                     /locus_tag="PLD05_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOP77162.1"
                     /translation="MERYYAATCQTNLPNPVDMAGIHRNVGRMLEMARFAVLDYEPLF
                     SVKLVVFPEFAQVAPVFGTVEELHDRLAVPVPNEFTDRYWDLAKELGIYIQTSSFLER
                     DDKYPGHVFNTTCLIGPPGILLKYRKVHPWIPGEWQTSPHDIPGYDEPLFPVADTEIG
                     KIGVAICYDWLFPEAIRQLALAGAEVLIRVSAYMDPWGTASPLEWWTLINRVRAIENL
                     AYVVACNQGASLRNYAPYSWPGGSMIVDYDGRILAQAEPGAGERIVVGPIDLAALRYE
                     RQRRRGHFMLGHLRMEAYSGYQKSIYPPGCFPRPPRISDIEQAIELGRQRWQQIAQKQ
                     R"
     gene            15118..15870
                     /locus_tag="PLD05_06715"
     CDS             15118..15870
                     /locus_tag="PLD05_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77163.1"
                     /translation="MLAQRVLKDVGLAAICVLLIIAVFYWRPFAAPDPTEQPAVGQPA
                     QFLEVYPLDKVLAAASAFPFQEPKPLTVEDLAGQVALVFFWNPRVDPSVDALRRLAAV
                     LPIVSRSDFRFLGVACPLQSDMETPGEFLSLTETTWKDCAIPLPCYLDPYGSSRTTLA
                     ILNQRGAEPAPRPAVVLPTAVLIDTDGLIRAVWEGWIPNQERDIARVVEKLLGPRATP
                     PEPPEGSTTSPHGGEAPTGHPSPEDASKPATG"
     gene            complement(15828..16604)
                     /locus_tag="PLD05_06720"
     CDS             complement(15828..16604)
                     /locus_tag="PLD05_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOP77164.1"
                     /translation="MCGRFTLRAAPQEVAKHFSLFDVPTFSPRYNIGPGQVVGAIRAK
                     ALEAPQAVAISDDMKLQPDGGEPSSPSVTPPREWAWFRWGLIPHWSSDPSIANRLINA
                     RAETVGSKPAFRSAARYRRCLIPADGFYEWKSEGRRKQPFFIRLRGDKLFAMAGLWDR
                     WHTPTGEVVETCTILTVQPNRVLAELHNRMPLILPPEAYSVWLDPSVTQFEALQDWLK
                     PYPEEEMELVPVSTYVNAIHNEGARCIQPVAGFEASSGEG"
     gene            16966..17838
                     /locus_tag="PLD05_06725"
     CDS             16966..17838
                     /locus_tag="PLD05_06725"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOP77165.1"
                     /translation="MAIPGLTIIGESINDSVPSTKALFDAGDLEGIVALAKSQVEGGA
                     AYIDVNVGSRGPELMAEVVRRVQGAIDKPLSIDTPDPVLARAGLTTYSPERAGGQKPI
                     LNSITVSREEMWELYALQPFCPILLCTEILVDGRPQAARTAEQTYEAAKFLVAEARRR
                     CPGIPNSDLIIDPGIVPIASDTEGNLKRLLGAMELIHNDPDLAGVHMSVGLSNFTVML
                     PPKRKDGSPVKSALESAFLTKAIPLGLDMIIGSVKRKYELLPPDHPAMQCLDAVLQAA
                     GFEAIMAVRKFYAE"
     gene            17942..18820
                     /locus_tag="PLD05_06730"
     CDS             17942..18820
                     /locus_tag="PLD05_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOP77166.1"
                     /translation="MPTSVLDELLKSAPVVLDGAWGTQLQMRGLPVGACPDEWNLSHP
                     ELVEEVARAYVAAGSQIILTNTFGSNPVTLQRHGLADQVVAINRAGVQISRRAAAGRA
                     KVFASIGPSGVMLMMGEKTPDELRAAFAVQAEALAQAGADGLVIETMSDPAEALLALE
                     AARATGLPVVVCMTFDSGKNRDRTMMGTTPEQAAEQLTAAGADVIGANCGAGPENYVE
                     ICRRLKAATDRPIWIKPNAGLPEVVNGQTVYRLSAEDFSKYVPQLLDAGASFIGGCCG
                     TTPEFIRAICRVLGRA"
     gene            18861..20093
                     /locus_tag="PLD05_06735"
     CDS             18861..20093
                     /locus_tag="PLD05_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP77167.1"
                     /translation="MNSRERVLCALNHREPDRVPIDLGGTRQSGIAATAYHRLKERLG
                     LRTPTRVYDVYQMLAEVERPILERFGADVIGLHRRCVAFGIPNQDWKPWRLFDGTPVE
                     VPGEFNPVTEPDGSLVLYNKSGEPIARMPKDGFYFDRLDKYPGAAHVDPEAQEIPVIS
                     QQECDELGAQAEALFHNTDFAIIVALGPPYELFFGLGTGDFTAWMITLATEPDYVRAL
                     YERITDVWLENLRRLTAALGDHVHIIQFNDDLGTQDGPFLSPRLFKELIFPYYKRGLD
                     WIHQNTNYKVFMHNDGAIYDFIPILIEMGVDILNPVQTTAKGMDPVRLKKEFGDRLTF
                     WGAACDCQRTLPFGTPEDVAREVEANIRVLAPGGGYVFASVHNIQANCPPENIIAMFD
                     TARTVGRYPIGVPQDQNS"
     assembly_gap    20108..20207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20208..20560
                     /locus_tag="PLD05_06740"
     CDS             <20208..20560
                     /locus_tag="PLD05_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOP77168.1"
                     /translation="GLLQTYEEWVAKYGEENARYLQEQLGELTRNYGKLTYIRTHTGC
                     DDRFEEIARQKASQRGWKFEVLEGDLRLIQALVDGEWNESEFLVVPPGGRIAPSFDDN
                     IIRPEMLTACPRDR"
     gene            20924..21952
                     /locus_tag="PLD05_06745"
     CDS             20924..21952
                     /locus_tag="PLD05_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP77169.1"
                     /translation="MNGYERVLAMIEGREVDHLPLMPITMMFAADVIGVPYGEYVSDY
                     RVLVEAQLRTAEEYGFDYVSCISDPAREAADCGAVIQYFDDQPPAIVEDQALLADKKK
                     LAQLKVPDPWGGGRMEDRVRAAELFRKKVGGQLFIEGWIEGPCAEAADLRGINTLMLD
                     FYDDPAFVRDLFDFILEMELAFAKAQIEAGADIIGMGDAAASLIGPKFYNEFVWPYEK
                     KMVDAIHQMGGRVRLHICGNTNKILEGMGKLGCDIVDLDFLVPMEAGRQAMGPDQVLL
                     GNIDPVKVLRNGTPELVAEKLAECHRASGKRYIVGAGCEVPRGTPDANMFAMRDYAIN
                     HRPDDFIS"
     gene            22025..23218
                     /locus_tag="PLD05_06750"
     CDS             22025..23218
                     /locus_tag="PLD05_06750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOP77170.1"
                     /translation="MLKTWSFHLPTFVRFGPGLIRKLGELAQEFGWNALLVGYKDQTG
                     MEEVYQRAQRSLRSARMHIFTFFEVGPEPDADLVPRGVEMYKKNRCEVVIGLGGGSVL
                     DVAKAIGALVKMEGVPWDFANANEGHRRITESAPIIAVPTTAGTGSEVTAVAVLSFSG
                     KGRHPDMPLKAALVGEGLHPKIALIDPELTVGKSASTTAACAADALLQAVEACVSRMA
                     NPFSTVLAGRAACLIAQSLRTALSQPQDIAARSDLALASCFSGAAYDVTGVTVAHAMA
                     QALGAVFGVPHGLAVAIAGPVALRFLQQTCAEPFAELGRQCHILANTPEEQAGRFLDT
                     VEQLFRDVGLPDKYTIPANAPKDYLDRLVHHALQATPEACTLTPVKVDESVLRDLFQK
                     ILSPA"
     gene            complement(23419..24087)
                     /locus_tag="PLD05_06755"
     CDS             complement(23419..24087)
                     /locus_tag="PLD05_06755"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOP77171.1"
                     /translation="MARHRHSVNSLELIPWLEGRLQRPLPGPMMGTKYAPRPSLGRDY
                     SKIPADARPAAVLILLYWHEGQWWLPLTLRRGDLPDHGGQVSFPGGAIEAGETSREAA
                     IREFCEELGVEHFRPQILGRLSSIYIQRSRFRVDPWVAVVKERPTMCPQPCEVAEVLE
                     VPLNHLLDPQNFGSHTREYHGQAYEAPHFTFGQHRIWGATCLILGEFVTLVEEFLLEK
                     SAAD"
BASE COUNT         5070 a         6953 c         6944 g         5384 t
ORIGIN      
        1 ctaagacccc cgattatcgg tggcgatgag gttctggcgg cccaaggctg ataacaggca
       61 atcttgggcg gcttttttct atttcattgc tcgtagaacc caaattcata agccgcatcg
      121 aacagggcca cgatattctc gggcggaaca ccggcctgga tgttgtgcac gttattgaat
      181 acgtaaccgc cgcccggctt ccagatctcc agatgacggc ggacatgttc gcggatttcg
      241 tcgggagagg ccgacggcaa tacgtgttgc gcatcgatgg ccccacccca gaaagccaat
      301 tgctggccgt attcggcctt gagcgtggtg gggtccatat ctcgagcact gatttggacc
      361 gggttgagga tatcgatgcc gttgtcgata agatcgggaa taaagctccg gcaggcgccg
      421 cacgtgtgat accatatctt ggcgtgcgtc tgcgaacgga tgaattgaac gagggcttta
      481 tgccgcggtt tgacgattgc ttggtaaaag ctgggactaa agagtggacc atcctgcccg
      541 gccaggtcat caccaatcat gatcacatca accaggtcac cgacttcctt taagaacaag
      601 cggaaccagt ccatccaaaa ccgcagtgtg cgatcgagta aggcggcaca gaattctggt
      661 tgctcgatca tgtcgatgaa ccaccgttcc agtccccgca ggtaccagca ggtttcatag
      721 accacgcccg agatgccgct gacaacggcg taaggagttt cccggcgaat gcggagtgcc
      781 tcctctcgta atcccacaaa ccgagacgga tcatctcctt tgggaaaagg atactcctga
      841 agatccttca tggtggcctt ggccaaggga tgataggtga tgtccatgta aagaccaccg
      901 tcttccggca tggaccaacg gacaccccat tcatccacga ggtcaagcca aactcgccca
      961 tcgcgttctg cccgcacaat ttcgcctttc cagctctccg ccggttttgc cgcgatatag
     1021 cgggtatcaa cgtgaaagcg ttcgagcacc gcttccgagg gacgggccaa ctgctggacg
     1081 gcgtccataa tctcgatcgt ctcttcccat cccaagtgct tcaacaaggc ctcgtaggca
     1141 aggcgatgaa tacccgtctg gtttcctccc aggtcaatag gaacacggtc cggttcctgg
     1201 tggttgagcg ccgccaaaag ccgttcccgc gatgtcattt tctctcgtgg catacggtgt
     1261 atctctcttg gttaagaatc ctcggatgtt cgaccacaaa ccgactcttt cgtctggatg
     1321 gtcccctgtc agacgatgct cgaagatttt ccagcacgaa atacccgtgc tttcgttttt
     1381 gggtaatgtg agcaaggctt gtttgatgaa gataacgcaa tggcagaccg cgaacaacta
     1441 ccgagttcgt atgcgacaaa cggacgtata atgagaagtt tgaacggccc caagagcgaa
     1501 cccgctagac aatcccttgg cctatcaggt atcacgaagc actatgcatg ttgaattcca
     1561 cgggaaaacc caggttgcct tggcgtcact tttagcggcc gctggtttga cggcagggaa
     1621 ggttgttgtc ggtattttga caaacagcct gggaattatt tccgaggccc ttcactcggg
     1681 cttggacctg gtggctacgg gaatgacgct ctgggccgtc cgcattgccg atcgaccggc
     1741 cgatgaagag catcgttacg gtcacgggaa atttgagaat ctttctgcct tggccgagac
     1801 ggccttattg ttgctggttt gcgtttgggt tttttatgag gccattcaac ggcttttctt
     1861 tcgagcagaa cttgaaatcc tccccagccc ctgggcattt ggcgtcgtca ttgcctcgat
     1921 tgtcgtcgac ctttggcggg cccatcactt gcgggaagct gctgagaaat attccagcca
     1981 ggccctggaa gctgacgcct tgcattttgc ctccgacgtt tggtcatcgt tggtcgtctt
     2041 gttggggttg acctgtgtcc tgctggctca gcgattggat tggccctggc tcatccatgc
     2101 ggacgccttg gcggccctca ttgttgccat cttggtttcc gtgatctgcg tgcgactagg
     2161 actacgtgcc atcgatgcct tgaccgatcg gacatctcgc tcgcttgccc atctggtgga
     2221 gcattcggca agccaagtga aaggggttga ggaggttcgc gacctccggg ttcggcagag
     2281 tggggccggc gtgtttgccg atatgactgt ttgcgtcccc cgcgagttgg gtctggctca
     2341 agcccatgag gtcgccacca acgtaacggc tgccatccga agtcaacttc ccgaggcgga
     2401 tgtcgtagtg cacgtggaac ccgtggcgag agaagacgaa gaccttgtca catccatccg
     2461 agccttggcc gccagtttgg gacttgctgt gcatgacgtg caggtcattt ccactcccgt
     2521 gggaaactca atcgaattac atgtggaagt cagccctgac ttaaacctgg aacaagccca
     2581 tgacctcgtc actcggcttg aatccaccat caagagcgat ctcccaaaaa tcgaatctgt
     2641 cctaacgcac atcgaaccga atggcgtgac cagagcagaa agcgaggctc gcgatcaagc
     2701 gccgatcaga acggaatcat tccatcaggc cattgccgat ttcagtcagg cctctgggac
     2761 acccatgaat ggacacgccc ttgtgacacg tcaaacaccg cagggggttt ccctgagctt
     2821 tcatctgcac ctggatggca cgctgccaat cggcacggct catgagatca gccggactct
     2881 ggagagctac cttcaccgga aatttccgga attgtcccgc attgttatcc acattgagcc
     2941 ccaccctccg gcggaagggc attcgcccga accaggccca ccacaggtat cctgaagtcg
     3001 cctaactctt gatcacggac catctgaact cttttgttga ttctcacctt cagaagaacc
     3061 agcctcttct gccggagcaa tcgcgtccgg gccgggcacc tggccctcgt caggcacggg
     3121 aatcgtccca gcagactcga caccctggtt tcgcgggttt cctgtgatcg tatcctgcga
     3181 ctgtgctgga gcattcggga ttgaagtccc ttcacttgtg ggctgctcgc gctgttgatg
     3241 atccgtcggc gacgggcttt cggcgggctt tggttgatcc ccgagacggt ccgcaggcga
     3301 aggagccgct tgttcggggg ttctgcggtc ttcggaacta cggccgccct ttttatcttt
     3361 gccgcctttg ttcttcttcc ctgatcggcc actgtggctt cccgaggtct taggcgtgct
     3421 gggacggatc accccttttg ccgcaaaata ctgcatcaag tcggcaaagc tccgcatcgg
     3481 ttccttgccg gtcagtacct cctcgctgag cggttccgcg ggagccttgg gttgttcgcg
     3541 ttcaggacgc cgtggcaatc ggacaggtcg ggaaataacg ggcatcccgt cctcggactt
     3601 cacgctactt tcttctggcc gggcaggccg atcctctcgg cgacggaagc gactccgccg
     3661 ttcccgttgg ccttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtaa
     3781 accaatatcg acaaaggccc caaaatccac cacgttgagg accgttccca tcaactccat
     3841 tcccggcgaa agatcctcca gcttgaggat cccctgtttg aagacaggtg ggggcaattc
     3901 ttcacgtgga tcacgccctg ggcggacaaa ctgtgctagg atatcccgca gggtaaagac
     3961 accgcaattc aactcggccg ccagagcttc cacgttgact tgattaatgc gggcggccaa
     4021 ctcggctcgc ttgtcggcct tcgtcagatc ctccgccgtg atccccagtt tttccatcac
     4081 ccgatgggcc agttcatagc tttccgggtg aatccacgtg gcatctaggg gattgtcccc
     4141 tcccgaaatt ttcaagaatc ccgcggcttg aacgaatgtc gcctcaccta tgccggggac
     4201 ctttttcaat tcctcgcggc tgcggaaggc cccgtgttcc tgacggtact cgtagatccg
     4261 tcgagccgtg agcttattga gtcccgacac atagcccaac aaagcgggac tggccgtatt
     4321 gacatccaca cccacatagt tgacgcacga ttcgacgact tcatccaacg actcgcggag
     4381 gtgtttctcc ttgagatcgt gctgatacat accgacgccg aggttggccg gatcgatttt
     4441 caccaattca ctcagtggat ccagcaggcg tcgcccaatt gatacggcgc cacggtacga
     4501 cgcctccaga ttggggagct cctctcggcc aatttggctc gtcgaataca cgctggctcc
     4561 tgcttcgttg acaatcgtgt aaccaatccc ccggtttttc agctcattct ccagtaactc
     4621 ggcgatgaac atctcagcca gtcgcgagcc gctcccgttc ccgatggcga tgcaggtcaa
     4681 ctgatacttg tcgatcagtt cacagacggt ctccttggcc atttcccggg tgtaaccctt
     4741 cttctcgacc aggtagatga gcttatgatc gagaacattg ccatattcgt cgagcgctgc
     4801 gagcttgcac ccgttgcgat aaccggggtc aatggccagc actcggcggt ccctcaccgg
     4861 tcgttggagc aaaagtcgcc gcagattttt ggcaaagacc ttgactgcgt gagcttcggc
     4921 cttttccgta agttcgcgcc gaatttccct ttctaaactg ggcaaaataa gccgattgag
     4981 ggcatctcgg gcacacccgc ggaggaaatc tgcgtgggga tggtctttcg ggacgcaaat
     5041 ttcctcaacg gctcgatcaa gggcctcttc gtccgccttg atcttgaccc ggatgatctt
     5101 gagggcctca cctcggttga tggcgaggac cctgtgaggc ggaatcgatc gaatggcctc
     5161 ctgaaaatcg aagtaatcag caaattcctt gatccgctgt tcctctttgc gacgcttttc
     5221 ggcctcttta gccgctgccc gcggatttgg cgtctttgcc gcattggtcg gcggggaagt
     5281 cgcggttgcc gcctgttgcg atgattctcc cgaggaagcg gccgggggca tcgcatcggt
     5341 cggttgttgc acttcggcgg gaccacttgg ggattcggct atgggagctg cctccccagt
     5401 ctcggtgttt tcggcattag gatgttcaga cgcttgcgct tcaagccgtt gctcagtctc
     5461 ccccgcagga gaagctgcct ctgacggccg ctccattcct tccctgctcc ctgcgagggg
     5521 caaatcctca gtttccccgg gcgccgcctc ggtggggatc gatcccgtct gatcgggttc
     5581 ggtagcagcg gtctcggaag caggctcgct agccaacgat gacccggccg cggcagtttg
     5641 ctcctccggt ggggtcgtca cagcttggac aactgagctt tccgaggccg gtgtcggtag
     5701 ctctgtggcc ggactgggag ttgggctttg gcctgcggga gaaggttgtt gcggagtggc
     5761 tgagacctct ggggcaatgc ggactgtctt aatgaccccg gtcttcttga ggatatcccg
     5821 cagtctggcc cgcaaggccg cgttttcgct aaacatctcg gccaggatgt ggcctgcccc
     5881 taagagtgcg tcggccccgg aaaggatttg cttgtccgga ttgacatagt cggccgcccg
     5941 tttgtcgagg tccgcgcaga tcgggtcggc attaaggatc tcttccgcga gatgccccaa
     6001 accccgctca cgagcaatgg tggccaaggt ttgctttttg gggcggaagg gaagatagag
     6061 atcatccagt cgtttcaggc tgttagcggc caaaatcttt ttggccaggg tgggtgtgag
     6121 tttgttttga gcctcaatcg cccggaggat cgcctgcttg cgctccgcga gttgccgcag
     6181 cttcgtgaca cggttttgga tcgtccgaat ctgctgctcg tcgagtcccc cggtctgatc
     6241 cttgcgatac cgcgtgatga aggggaccgt gtttcccgcg tcaagcagtt ggacaacagc
     6301 ctgaatccgt ttctccggaa tctgtaattc ccgtgacaat ccgtgaaaag ataccgtaac
     6361 ttcagttgtg gccataagct ctcgggcgat tgtctttcca aagcgctcgc gtgcttcgac
     6421 accgagaatc ctccaccttg ggatacccag gaggagactc ccgcggtggg acgaacttag
     6481 gaccagtgct cctgaggcca ggtgccctgg tcaccgagac tcggtaaccc tgtcatacga
     6541 tctgatattc agatacaacg cgtcgcgcaa cctcctcaac gtccctctca caaataaccg
     6601 aattgactgc caaaaatctc tctaccccaa ctgattgagt ataaccgatt tggtgaggtt
     6661 ctgggtacgg gaagtggctt agcctgtttc aagaaaaaac acgacaccac cctttagaag
     6721 gtcgcgttgt tgtgtctggc tccaggtcca atcaagttcc caaaacagcg aaatcaaccc
     6781 gtccctaccc ggagtcgtcc ctttaagagc ttatccgcaa aagtctccct ctccctccgg
     6841 gagagggctg gggtgagggc aagcgaacgt cggagtgggt cggaaaaacc agcagaaatt
     6901 cgcgccactc tcacctccgc cccctctccc gccaaacggg cgaggggaga ttatggagtg
     6961 gacgttaagt acgggatcat cacagccggg gcataccaaa tattatcctc gattgcccac
     7021 gggaaggcgc ggaacaaggc ctttccggta aaatagagga aacagcattc ggcagatcgg
     7081 ccagttttcc caggagcatc cgagcgtatg gaaggtatca gtggatggga catcgccctt
     7141 tggagtgtgg cggccttcgc tgctgtgatg ctcttggtgc gggtcatgct gtcatttcga
     7201 cagtactggg ttgagtatta ccggatgcag gccgaggcag ctcgaaagcg aaaaccgaag
     7261 aaagaggggt gaacccgggt ctctcgcgtc ggccccaagg ctgatctccg tttgtggacc
     7321 ccacagcatc tgaccgcctt ttactcgatc atgtgaacgt ctgcacaaca caatgggaaa
     7381 acgtttttac attgaaaccg tgggctgtca aatgaatgaa cttgacagcg aactcgttat
     7441 tgcagccctc ctcgaagcgg gctacgagta cgtcccccgc cggcaggatg ccgatattat
     7501 cctcttcaac acgtgtagcg tccggcagca cgccgaagac aaaatctaca gtgccctagg
     7561 tcggctgaaa cacctgaaag agcaaaaacc cggaaaaatc atcggcgtct tgggctgtat
     7621 ggcgcaaaag gatcaaggtt tgatcctcaa gagggcgcct catgtggacc tggtcgtagg
     7681 cccagggcaa gttgcccggt tacccgagct cctgcaaaag ctgggcgagt ccggttctcc
     7741 cattgtggaa gtcagcctag accgccgcgg ggtttctcaa caacgagttc gcgcgagttt
     7801 cctcccctta gatcgcaagg agcgtccgtc tgtccgcgtc acgccctacc aggcttatgt
     7861 gcgggtcatg tacggctgcg acaagttttg tacgtattgc gttgtgccgc gagtacgggg
     7921 tccagaacaa agccgatccc ccagtgaaat cttggatgaa gtccgccaat tggccgatca
     7981 agggtgcctt gagatcacgc tgctgggtca aacggtcaac agttatcact ggaccgaagg
     8041 cgggaagacc tggcggttgt ccgatgtcct ggcagaagtc catgaggtag aaggcatccg
     8101 ccggatcaag ttcgtgacga actatccctg cgatatgacg cccgatctcc tccaagcggt
     8161 gcgcgacctt cctaaagtgt gtccgtattt gcacgttccg gcccaaagcg gttccaaccg
     8221 gattcttcag aggatgaagc gagggtacac tgtggaggag tatcgagaga tgctcgcggc
     8281 catccgggag acgatcccag gcgccgccat aacgagcgac tttattgtcg gtttttgtgg
     8341 cgaaacggaa gaagattttg ccgccaccgt caaacttgtg gaggataccc gctttaagaa
     8401 cagtttcatc ttcaagtaca gtcctcggcc aggaacgaag gccgccgaac tttggccgga
     8461 tgatgtgcca gaagaggtca agcgagatcg caacaacgag cttttggcta tccagaaccg
     8521 tatcgcagag gaagagaatc gccgctttgt gggacaggtt gtggaggtgt tggtggaggg
     8581 ccccagcaag gccagcggca aacagcggca atcggacggt tcactacaat tgacagggcg
     8641 cacggcgtgt gatcggattg tggtatttcc ggggtcatcc gacctggtgg gtcagtttgt
     8701 ttccgtaaag atcgaggcgg tgagcgccgt aacattgttt gggcatgtgc tggcggcgga
     8761 agctgccgtc tcttgaaatg aattccggac cgtcgcggcc tggaaagcca tcctttgcag
     8821 caaatctgac attgtttatg gaagtcgaaa cgttacgttc gtttttggag aaccctgaag
     8881 gagccaaccg ttggttgtcg ggccttcgtc tgcgtgatcc ccgtgttgcc catcggaacc
     8941 tcctcagcat cgcccggcag ggagtcccgc tggacttgct gacggtgctc atccagcaga
     9001 tggaaaaggt gcttccggag ttggccgacc cggacatggc gctcaataat ttggaacgtt
     9061 ttttcgccgc tacccgcaat ccgctgtcgt tagcgtcact ttttgaacgt gatcccgagg
     9121 ccctcgcccc tttgctgcag attttctcgg ccagtcagtt ccttagcgaa atcctgatct
     9181 gtgatccgga aagctacgac ctgctgcggc tgaccgaggg ccaacccgtt tcccgccagg
     9241 ccttgatcga cgagctaaca tcggaaatcg ccgcgctgac ccacgaaaac gagatcatgg
     9301 ccacgttgcg gcggttcaaa cgacgggaaa tattgcggat tgcctacggg gatttcgtct
     9361 gtggtcacag cctttccacg gtcacacggc aaatatcgta cgtggcggat gccctgttgg
     9421 aagcggccta ccaggccgcc cgaaaaaaac tccgcggcaa actgggagag cctcgcacgg
     9481 tcgatggctc ggtcgcccgt ttcgtcatcc tcgccatggg aaaactggga ggagttgaac
     9541 tcaattattc cagcgacatc gatctgattt tcctctatga ggacgaggga acaaccgatg
     9601 gcccgcgttc cgtgagcaac gccgaatact tccatcgact ggttcaggaa atcgtgcgct
     9661 ttctcaccct gcggaccgaa ttgggggccg tttaccgtgt cgatcgtcgc ttaagtccgg
     9721 aaggcgaaca gggaccggcc gccatcagtg taccggcagc tcttcgttac tacgacatgc
     9781 gagggcgaac atgggaacga caggcctaca tcaaggcgcg accagcagcc ggaaacctag
     9841 accttggata cgccttcctg gaacaactag aaccgtggat ttatcgccgc tatttgacgc
     9901 gggccgatat ttcgggaatc aaaactcttc gccgaaaaat cgaacagcga gctcgggccc
     9961 aaggcgatct ccgcgatgta aagaatggtt ccggcggcat ccgcgacatc gaatttgtca
    10021 tccagttttt gcagcttctt cacggtgccg atctccccgg cgtgcggacc ggcaacacgc
    10081 tcgatgcgat tgctgggctg gaacaggctg gttgcttgac tcacacggag agcgcttatc
    10141 ttcaagagag ctatcacttt ctccgcaaac tcgaacatcg cctccagatc atgttcgact
    10201 tacagacgca cacgatcccc cagggcgcgg aagaaatcca aaaactcgcc gtgcggatgg
    10261 gctatcacga tacgcctacg aggtcggcca gcgaggcctt tgaggcagat tactatcgct
    10321 tcaccttccg caatcgacaa atcattaacc acctgctcca tgatgcgttt cgcgatgaaa
    10381 cggagacatc cccagaggtg gaattggtgc tcgatcccga gccatccccg gactttatca
    10441 agcaggtctt aagccgtcat cgcttcagca atccagagca ggcctaccgc aacctcatgt
    10501 cgctcgccga agagcgcgtg ccgttcctgt ccacgcggcg gtgccgacac ttcctggcct
    10561 ccattgcccc gatgctcctt tcggcgatcg ccacaacccc cgatcctgac gccactttgg
    10621 tgacgcttga ccaagtcagc gattccctcg gtggaaaagg cgttctgtgg gagcttttta
    10681 gtttcaatcc gccgtcgctt aagttgtacg tccagttgtg tgcttacaca ccattcttgg
    10741 ccagtctcct catcagcaat ccggggatga tcgacagcct aatggacagc cttgtcttgg
    10801 accgactgcc aagccgggag tttatggaaa actcccttcg tgagctatgc ctagccgcag
    10861 aagacatcga tcccatcttg cacgccttca agaatgacca cctcctccag attggcgtgc
    10921 gtgaccttct tggcaaggag gacgtcgagg ccattacgcg gaccatcagc gaattggccg
    10981 aaacttgtct catggagatt gttgtccggg aatacgaaca cctggtgctg cggtatggcc
    11041 agcccaccat ccaagaaggc cccgatcaag gacgccgcgc ggagatggtg atcctcgccc
    11101 tcggaaagct gggcggtcag gagatgaact atcaaagcga tctggatctc gtgtttcttt
    11161 acgagggcga tgggcagacc gtgcctgttg tcgattcggg ctacgtccgg cgaggggaag
    11221 tgaccaccaa ccaacatttc tttagcgagt tgacgcaaag gatcatacgt cgggcttcct
    11281 acctcggtcc tcatggcaga ctttaccagg tggacgcacg gcttcgcccc acgggacgga
    11341 gcggcttgct ggtattcacc ctcaacgagt tccggcagta ctttgaaagt ggtcagggtc
    11401 aattgtggga acggcaggcc ctatgtaagg cccgtccggt cgtggtcaca ccccggatgc
    11461 agcccgtcat caaccaactc ttgcacgaag tcattcactg ccagccctgg cgaccggaat
    11521 tcgcccacga gatcgcagcc atgcgcaagc gattggaagc cacggctaaa gggttagata
    11581 tcaaacgcgg ttgcggaggg actgttgatg ttgagttcct ggttcaaatg ctgcaactgc
    11641 gtcacggacg ggaatttccc caactgcaca caacatcgac catggccgcc ttgcggatgt
    11701 tgcgggaatg cggcgtgctt gaggaaagag acttcaagga attgttcgaa agttaccggt
    11761 atctccgaac acttgagaac cgcatgcaac tggtggcgcc caccgctcgt agccgcttgc
    11821 ccgataaccc cgtggaactt tctcggctca gccacctcat gggggccgaa acaccccctg
    11881 ccatgatccg caccgtggag gcttatctcg cccgtaatcg gtcccacttc gaacgtgtcg
    11941 tggaaaagct tgccaacgag cggatcgccc tcccctcatc gcccaaccaa gaaacaccgc
    12001 atgataacta aagctgccag aacgctggcc aacgttgtgt gttgttgtga tttgggaggt
    12061 taaaccttgt atcgcattgc tattagcaag caggtcaaat catgcaagat caaccgcaac
    12121 ttgatccgga aggccgtgcg ggtcgtcctt caacaggcgg gcatcgaaac cgccgaaatc
    12181 agtattgcca ttgtggatga cgccacgatt gcccagcttc atgggcaata cctgggagac
    12241 ccgaccccta ccgatgtgtt aagttttgtc ctggaagaag gcgaccacca cttggagggc
    12301 gagatcgtcg ccagcgcaga aacggcggct gcctgggcaa aacgtctcgc ctggccttgc
    12361 gaaaacgagt tgttgctcta cattgtgcac ggtacactgc atcttgtagg cttcgatgac
    12421 caaaccccgg tggcacgaag aaaaatgcgc cgcgaggaaa aagcagttct tcacaagtta
    12481 ggtgttgttc cgccggttcg cagccgccgt tagtcttttg ccatgcgata accggcaatt
    12541 aagctccgtg gtttggtcac aatccgttaa cgatgaggaa ctacctgacg tgtcggagga
    12601 ccaagtgctt ttatggctgt gtgttttggg actgggcata tcgtgcgtcg cttccacagg
    12661 aacacgtgcc ctttccgatt tccccagcca tgacctagag gagctctgtc gtaaacgcaa
    12721 tcgcatggag cgatttcggg aagtgctctc gcgctaccgg cgggtctcta tcgccagtga
    12781 gttggtctcg ctcctcgccg tcctcgtcgt aaccgtcgcg gcggtcatcc tgggaagcca
    12841 actttggcac gaaatcaagc aggcagaacc ctctgcgagg ctggtcctct gccaaaggct
    12901 tctcctagaa gggcttggtg gtgtgctggt gatcttgatg ttcactgtgt tcatcccgcg
    12961 ggccgtttca ctgctttggg ccgcccgggt tgtctattat gcctggccat tctggcggtt
    13021 tttagagcga ctcatgaccc cattgctttt tgtgaaccga gttgtcgaag tcatccttgc
    13081 ccgcagcgcc ggcgtgcaat taccccagga gtccgaggaa caattcgagg aggaaattcg
    13141 cacgctcgtc agcgaggggg aacgggaagg gcttttggag ggcgaagccc gcgagatgat
    13201 cgagggcgtt atcaagttgt ccgatgtcac cgtctccgag atcatgacgc cgcggactga
    13261 aatgatgtgc ctctccgcga aactgagctg ggacgaaatg cttgagagcg tcatcaacga
    13321 aggacattcc cgtatccctg tttacgagga aacccgtgat aacatcatcg gaatccttta
    13381 tgtgaaggaa ctcttgccat atttggcgcg gtccgcagaa caggaacgtc cgccttggac
    13441 gtcgcttctg cgcgagcctt tttacgtccc tgaaaccaaa cccgttgatg aacttatgca
    13501 agaattccag cgaactcgca atcacatggc aatcgtcctt gatgaatatg gcggcgtctc
    13561 cggtctgatc acgctggagg acatcttgga ggagatcgtc ggcgagatcg gcgacgaatt
    13621 ggaccaggag gtcgtggagg agattcgcat cgacgaccag gggcgggccg aggtccaggg
    13681 taccgctcgt gtggaagagg tcaaccgtcg gctggatctt gggctgccgg aaagtgagga
    13741 gtacgacacc atcgcgggct tcctcatgac tcacctgggg agggtccctc acgttggcga
    13801 atcgattgtc tttggcaagt ttcgcatcaa ggtcttggat ggtgatcgcc gccgaatccg
    13861 tcgggtgatc atcgaaccgg ccgagccagt agcgttgccc gaggctcaaa actgagttcg
    13921 ttcgctatct ggtattgtgc ttcaagggaa tcatgtccgg gactgtggca aggaaaggat
    13981 gatcctttgg aacgatatta cgcggctacc tgtcagacaa atttgcccaa tccggtcgat
    14041 atggccggca tccatcggaa cgttggccgg atgctggaga tggcccgctt tgccgtcctc
    14101 gattatgagc ccctgttctc ggtgaagctg gtggtctttc ccgagttcgc ccaagtggcg
    14161 ccggtgtttg gtacggtgga agaactccac gatcgcctgg ccgtgcctgt ccctaacgag
    14221 ttcaccgacc gatattggga cctggccaag gagttgggaa tctacattca gacgagcagt
    14281 tttttggaac gcgatgacaa gtatcccgga catgtcttca acaccacatg tttgattggt
    14341 cctccgggga tcctcctgaa ataccgaaaa gtgcacccgt ggattcctgg tgaatggcaa
    14401 accagcccgc acgacattcc cggatatgat gagccgcttt tccccgtggc ggatacagag
    14461 atcggcaaaa ttggggtggc aatctgttac gactggcttt tccccgaagc catccgccaa
    14521 ttggccctgg cgggcgcgga ggtccttatc cgtgtctctg cctacatgga cccttgggga
    14581 acggcctcgc ctttggaatg gtggacgttg atcaaccggg tgcgggccat tgaaaacctc
    14641 gcctatgtgg tggcctgcaa tcagggggcc tccttgcgga attatgcgcc gtatagctgg
    14701 ccaggcggaa gtatgattgt cgattacgat ggccgaatcc tggcccaggc cgaacccggg
    14761 gccggggaac gtatcgtcgt gggccccatt gatctggcgg ctttgcgtta cgagcggcag
    14821 cggcggcgcg gccacttcat gctggggcat ctccgcatgg aggcctacag cggttatcag
    14881 aagtcgatct atccccccgg atgctttccc cgaccgccgc gaatcagcga cattgagcag
    14941 gccatcgagc tgggcaggca acgctggcag cagatagctc aaaagcaacg ctgaaatgag
    15001 tgtttcccct gtgaggcacg tcccgtgtta aacccattga ggcgtcgtat tcttcgtgcc
    15061 tttgcattcc ctcgcagaga gcctctccgc ggcgggccaa ggagagcctc tcaagtcgtg
    15121 ttggcgcagc gggttcttaa agatgtgggg ctggcggcga tctgcgtcct tttgatcatc
    15181 gccgtttttt actggcggcc gtttgccgca ccagacccaa ctgagcagcc tgcggtgggc
    15241 cagcctgctc agtttctcga ggtttatccg cttgacaaag tccttgccgc ggccagcgca
    15301 ttcccttttc aggagccaaa acccctgacc gtggaagacc ttgcggggca ggttgccctt
    15361 gtcttttttt ggaacccccg ggtcgatccc agcgttgatg ccttacgccg cctggctgcg
    15421 gttcttccta tcgtctcgcg gagcgacttt cgctttctcg gcgtggcctg tcccttgcaa
    15481 agtgacatgg aaacccccgg cgaatttctc tctttgaccg aaactacctg gaaggactgt
    15541 gccatcccac ttccttgcta tttggaccct tacgggtcga gtcgaacgac gttggcaatc
    15601 ctaaatcaga ggggagccga accggcgccg cgacccgccg tcgtattgcc cacagcggtc
    15661 cttatcgaca ccgatgggct gattcgcgcc gtttgggagg gatggattcc caaccaggag
    15721 cgcgacatcg ctcgagtggt ggaaaagctc ctcggcccac gggcgacgcc tcccgaacca
    15781 cccgagggct ccacgacgtc accccatggc ggcgaggcac caactggtca tccttcccca
    15841 gaagacgcct caaaaccggc caccggttga atgcagcggg caccctcatt gtggatggcg
    15901 ttgacgtagg tactcaccgg caccagttcc atctcctctt cgggataggg ttttagccag
    15961 tcctgtaatg cttcaaactg tgtcacggag ggatcaagcc acacggaata cgcctcgggt
    16021 ggcagaatga ggggcattcg gttgtgcagc tctgccaaga cacggttcgg ctgcacggtc
    16081 aaaattgtgc acgtctcgac aacctcgccc gtcggtgtgt gccaccgatc ccataaaccg
    16141 gccatcgcga aaagcttgtc accccgcagg cgaatgaaaa agggctgttt ccttcggcct
    16201 tcgcttttcc actcgtaaaa accgtcggcc ggaattaaac atcgacgata ccgcgctgcg
    16261 gagcgaaacg ccggcttgct gccgacggtc tccgctcggg cgttaatcag ccgattcgca
    16321 atggagggat cgctgctcca gtgcggaata agtccccagc gaaaccaggc ccattcccgc
    16381 ggtggcgtta ccgatgggga tgatggctcg cccccgtcgg gttgcaattt catgtcatca
    16441 gaaatggcaa cggcttgggg tgcctccagg gcctttgcgc ggatggcacc gacaacctgt
    16501 cccggcccaa tgttgtagcg gggcgaaaag gttggaacat cgaacaacga gaaatgcttg
    16561 gcgacctctt gcggggccgc ccgaagagtg aatcgtccgc acataggctt tccttacggc
    16621 atctggcgat gggcccgtcc gccctgtacc cccattgtat cgcccactcc gccagacgcg
    16681 acggcgcccg tccgccaaag tttatcgtag cggctgccct ccatgcctgc ccatttcttc
    16741 cctggcgtcg gcgggggtgc aagaaagccc gcgagccaca aaggttaccc ctaccagtcg
    16801 atcacgatca tcctgggagc cgtgcaccgt cgtagccggg gacgacaatg gacatcattg
    16861 gtacgacggc ctatcggcct gtggaaaggc cttccaaaag atgctaaaaa aacgatttac
    16921 cttgcagttg acagccgggt gtgacgttag gataggagac acacgatggc cattccggga
    16981 ttaacgatta ttggggaatc gatcaacgat tcggtcccct ccacgaaggc ccttttcgat
    17041 gcgggagact tggaggggat cgtagccttg gccaagagcc aggttgaagg cggcgctgcc
    17101 tatatcgatg tcaatgtcgg ctcgcgcggt ccggaattga tggcggaggt cgtacggcgt
    17161 gtccaaggtg ccattgacaa accgttgtct atcgacacgc ccgacccggt acttgcccgg
    17221 gcaggtctta ccacttactc gccggaacgg gctggtggcc aaaaaccgat cctcaattcg
    17281 atcaccgtct cccgagagga aatgtgggaa ctttacgccc ttcaaccgtt ctgtcctatt
    17341 ctgctttgca cggaaatcct agtagatggg cgtccccagg cagcccggac agccgagcaa
    17401 acctacgagg ccgcaaaatt tctcgttgct gaggcccgtc gtcgttgtcc gggcatcccc
    17461 aacagcgacc tgatcatcga ccccgggatt gtgcccatcg caagcgacac cgaaggaaat
    17521 ctcaaacgat tgttgggcgc gatggagttg attcacaatg atcctgacct agcgggggtc
    17581 cacatgtcgg tgggtttgag caatttcacc gtgatgttgc caccaaagcg gaaggacggt
    17641 tcgccggtga aaagcgccct ggagtcggcc ttcttgacaa aagccatacc attgggtttg
    17701 gacatgatca tcggctcggt caaacgaaaa tacgaattat tgccgccgga tcacccggcc
    17761 atgcaatgct tggacgccgt cctccaggcc gcgggcttcg aagcgattat ggccgtgcga
    17821 aagttttacg ccgagtgacg tcgtatccaa gcgatgtcct accgcaagga tcggcaattg
    17881 tggatcttgc tccagacgac aaataatgcc atcacgaaag ctgggagaaa ggacctcacc
    17941 tatgccaacg tcggtgctcg atgaactcct gaaaagtgcc ccagtggtgc tagacggcgc
    18001 ttgggggacg cagctccaaa tgcggggcct accggtgggc gcatgtccgg atgaatggaa
    18061 tttgtcccat cccgagctgg ttgaagaagt cgcgcgggcc tacgtggcgg ccggaagtca
    18121 aattatcctg accaatacgt ttggctccaa tcccgtgacc ctccagcgcc acggcctggc
    18181 cgaccaggtg gtggcgatta accgggccgg cgtgcagatt tcacgccgtg cagccgcagg
    18241 aagggcaaag gtctttgcat ccatcgggcc atccggggtc atgctcatga tgggcgaaaa
    18301 gacgccggac gaactgcggg cggcctttgc cgtgcaagca gaggccttgg cccaagctgg
    18361 ggccgatggc ctggtgatcg agacgatgtc cgaccccgcc gaggcccttc tcgccttgga
    18421 agcagcccgg gcgaccggtt tgccggtagt cgtttgcatg accttcgact ccggaaagaa
    18481 ccgtgatcgt accatgatgg gcacaacacc ggaacaggcc gcggaacaat tgacggcagc
    18541 gggtgccgac gtcatcggag ccaactgcgg tgccggtccc gaaaactacg tggaaatctg
    18601 ccgccgtttg aaggcagcca ccgatcgtcc catctggatt aagcccaacg cgggtttgcc
    18661 ggaggttgtc aacggtcaga cggtctatcg cctttctgcc gaagatttct cgaagtacgt
    18721 tccgcaactg cttgacgccg gggcgagttt cattggtgga tgttgcggaa caacgcccga
    18781 gttcattcgg gcgatttgcc gggttctagg tcgtgcttag agacgcttct tttttcacca
    18841 atttttccgg accaggaacg atgaactctc gtgaacgagt cctctgcgcc ttaaaccatc
    18901 gggaacccga tcgcgttccc atcgacttgg gtggaacgcg ccaatcgggg attgctgcca
    18961 cggcatatca tcgtttgaaa gaacgcttag gactacgcac accaacccgc gtttatgacg
    19021 tgtatcagat gctggccgag gtcgaacgac ctatcctgga acgctttggg gccgacgtca
    19081 tcggactgca tcgccgctgc gtggctttcg gcattcccaa tcaggactgg aagccatggc
    19141 ggcttttcga cggcacaccg gtagaagttc ccggcgaatt caaccccgtg actgagcccg
    19201 acggttcttt ggtgctgtat aacaagtcag gagagcctat tgcccgcatg cccaaggacg
    19261 gcttctactt tgaccgtttg gacaagtatc ccggggctgc ccatgttgat ccggaagccc
    19321 aggaaatccc ggtgatctca caacaagaat gcgacgagct gggcgcccag gcggaggcct
    19381 tgttccacaa tacggatttc gccatcatcg tcgctctcgg accgccttat gagttattct
    19441 ttggccttgg gacgggcgac ttcacggcat ggatgatcac ccttgccacc gaaccggatt
    19501 acgttcgcgc cttgtatgag cgaatcaccg atgtttggct ggagaatctt cgccgattga
    19561 cggcggccct cggcgaccat gttcacatta tccagttcaa cgacgacctg ggaacgcagg
    19621 atggcccctt cctttcacct aggcttttca aggaactgat ttttccctac tacaagcgcg
    19681 gccttgactg gattcaccag aatacgaatt ataaagtctt catgcataat gacggcgcga
    19741 tttatgattt catccccatc ttgatcgaaa tgggtgtcga tatcctcaat cccgtccaaa
    19801 ccacggcaaa gggaatggat cccgttcgat tgaaaaagga gttcggcgat cgcctcacct
    19861 tttggggtgc cgcgtgtgat tgtcagagga cgctgccctt cgggaccccc gaggacgttg
    19921 cccgcgaggt tgaagccaac atccgggttt tggcacccgg tggcggctat gtgttcgcct
    19981 cggtacacaa catccaggcg aactgtcccc cagaaaacat cattgccatg tttgatacgg
    20041 cccgaactgt cggacgttat ccgatcggag tgccgcagga tcaaaacagt tgaatctttt
    20101 gggcggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg gacttttgca
    20221 aacgtatgag gaatgggtcg ccaagtacgg tgaagaaaat gcacgttatt tgcaggaaca
    20281 acttggcgag ttaacgagaa actatggaaa actgacatat atccgtaccc acacgggctg
    20341 cgatgacagg tttgaggaaa tcgccagaca aaaggcgtcg caacgcggct ggaaattcga
    20401 ggtcctggag ggcgatttgc ggcttattca ggcccttgtc gatggagaat ggaatgaatc
    20461 ggagtttctg gtagtccctc ccggtggccg cattgccccc tcgtttgatg acaacatcat
    20521 ccgcccggaa atgctcacgg catgccctcg tgaccggtag ggcaaccgtt gtggttaccc
    20581 cgtcctcgtc cccacaccgc gcagcaatga aaggaacggg caggcaccag tcgcctccag
    20641 gtggttggaa aacgcgcaga tgatgacatc gcactctacc tttcggttgg agttcgggat
    20701 gtgaccaaat cgcaagctag tcgtcgcggt gctattgatt ggttaggagc tggatccaaa
    20761 gactctcacc ggttgcggga aggccgttcg gctactatga tgaatccccg gcactccaca
    20821 atggtcatcg gcggtgtctc tccgcgctga acacgatctt ttgcgacgac cactcgatgg
    20881 aacgcccagg ccaggacatt ccgaccaaat ggaggggtga ttgatgaacg ggtacgagcg
    20941 cgtattggcg atgattgaag gtcgggaggt ggaccatttg ccgctaatgc ccattaccat
    21001 gatgtttgcc gcggacgtca tcggggtgcc ctacggcgag tatgtaagcg attatcgtgt
    21061 cttggtagag gcccagctcc gcacagccga agaatatggg ttcgactacg tttcctgcat
    21121 ttcagacccc gcccgggaag cggccgattg tggtgccgtg attcagtact tcgacgatca
    21181 accaccggcg atcgtggagg accaggccct gcttgccgac aaaaagaagc tcgctcaact
    21241 taaggtccct gatccatggg gtggaggccg gatggaagac cgcgttcggg cggcagaatt
    21301 gtttcgtaaa aaggttggtg gtcaactctt catcgagggc tggatcgaag gtccctgtgc
    21361 cgaggccgcc gatctccgcg gtatcaatac tctgatgctt gacttctatg acgatcccgc
    21421 ctttgttcgt gatttgttcg atttcatctt ggagatggaa ctggccttcg ctaaggccca
    21481 aatcgaggcc ggggccgaca tcatcggcat gggagacgcc gccgcatcat tgatcggccc
    21541 gaagttctac aatgagtttg tttggcccta cgaaaagaag atggtggatg ccattcacca
    21601 aatgggtggg agggtccgcc tgcacatctg tggaaacacg aacaagattc tggagggcat
    21661 gggaaagctc ggttgcgaca ttgtcgattt ggatttcctt gtgcccatgg aggccggccg
    21721 ccaagccatg gggcctgatc aggtccttct cgggaatatt gacccggtga aggttctacg
    21781 gaacggcacg ccggaattgg tcgccgagaa actcgccgag tgtcaccgtg cttcgggaaa
    21841 gcgatatatc gtgggggccg gttgcgaagt cccccgaggg acgccggatg ccaatatgtt
    21901 cgcgatgagg gactatgcca tcaaccatcg tccggatgat tttatctcct aaaatcaatt
    21961 tgacacacct tgtaagcctt gcttgatagt gtcattgacc gcatcggcct ataggagtgc
    22021 ttccatgctt aagacgtggt cgtttcacct gccgacgttc gttcgctttg gaccagggtt
    22081 gatccgaaaa ctgggagaat tagcccaaga gttcggttgg aatgcgttgc tggtcggtta
    22141 caaagaccag accggcatgg aagaggttta tcaacgggca cagcgctcgc tccgctcggc
    22201 gcggatgcac atctttacct ttttcgaggt tggaccggaa ccagacgccg atctcgtgcc
    22261 ccgcggcgtc gagatgtaca agaagaaccg gtgcgaggtg gtcatcggat taggaggcgg
    22321 cagtgttctt gacgtggcca aggctatcgg cgcgttggtg aagatggagg gtgtcccatg
    22381 ggactttgcc aacgcgaacg agggacaccg gaggatcacc gaatcagccc ccatcatcgc
    22441 tgtccccaca actgctggga cggggagtga ggtaacggct gtggccgtgt tgtcgttctc
    22501 gggcaagggt cggcatccgg atatgcccct caaggcagcc cttgtgggag aaggactcca
    22561 tcccaagatc gccctaatcg atccggaact taccgtgggc aagtcagcct caacgacggc
    22621 cgcatgtgcc gcggacgcct tgctccaagc ggtggaggcc tgcgtcagcc ggatggccaa
    22681 tcctttctcg acagtcttgg ccggtcgtgc tgcttgtctc atcgcgcaaa gcctgagaac
    22741 ggccctcagt cagccgcaag acattgccgc gcgatccgat ctcgccctgg cttcgtgctt
    22801 ttcgggcgcc gcctatgatg tgacgggcgt cactgtggcc catgccatgg cccaggcctt
    22861 gggggctgtg tttggagtgc cacacggttt ggccgtggcc atcgctgggc ccgtggcgtt
    22921 gcgattcctt caacaaacct gcgcggagcc cttcgccgag ttgggacggc agtgtcacat
    22981 cctggccaat accccagaag aacaagccgg tcgattcttg gacacggtcg agcagctttt
    23041 ccgggacgtg gggctacccg acaaatacac gatcccggcc aacgccccaa aagattattt
    23101 agaccggctg gtgcatcacg ccctccaggc cacaccggag gcctgcacct taacgccggt
    23161 aaaggtcgat gaaagcgtcc tgcgcgatct cttccaaaag atcctcagcc ccgcgtgacc
    23221 cggtcagtac agggccttcg tctcactagt ttgcattttg cggctcatgg ggccaagtta
    23281 gctgcgaggt cgccgccttg cgagttctcg ccgtaaggtg gggctgtgta ttcatcatgg
    23341 atgcgcttgc cgatcactta taggagcggg tcctgtgctt gagcgtgttc agaaccgcta
    23401 tccgtgcctc cgccctcact aatcggcagc gcttttctcg agcaaaaatt cctccacaag
    23461 ggtcacaaac tcacccagga tgaggcacgt ggcaccccag atgcggtgtt gtccaaaggt
    23521 aaaatgcggc gcctcataag cttgaccgtg atactccctg gtatgtgaac cgaagttctg
    23581 cgggtctagc aaatggttaa gagggacctc caggacctcg gcgacttcac acggttgcgg
    23641 gcacatcgta ggacgttctt ttacaacggc cacccaggga tcgacgcgga agcgacttcg
    23701 ctggatataa atcgaggaca gccgcccgag aatttggggg cgaaagtgct ccactccaag
    23761 ttcctcgcaa aactcccgga tcgccgcttc gcgggaagtc tcaccggctt cgatggcacc
    23821 acccggaaaa ctgacctgcc caccatgatc cggtaaatcg cccctccgta gtgtcaaggg
    23881 cagccaccac tgcccctcgt gccagtaaag aaggatcagc acggcggcgg ggcgggcgtc
    23941 tgcgggaatc ttcgagtagt cgcggcccaa actcggtcgc ggcgcgtatt ttgtccccat
    24001 catcggccct ggcagaggtc tttgcaaacg tccctcaagc caggggatca attccaacga
    24061 attgacggag tgccgatgac gggccatatg cgatacgcac ttcggcaagt ttgcctatcc
    24121 aacaaaactt aacaagagtc tatccccaaa agtccccctc tccctccggg agagggttgg
    24181 ggtgagggca agcgatcgcc gaagtcgatc gagaaaaccg gcagcgactc gagccaccct
    24241 cacccccgcc ccctctcccg ccgaacgggc gaggggagat tatgggatag gctctaacac
    24301 gattctgaag ttttcacttg agtcggcagg acagcacacg ggggtgtggc ctgcccgttt
    24361 catttttagg gagttgtctc cttaatggca catcatgggc caccggtccc caaagtatcg
    24421 atctcgataa cgccgccttc ttttctggcg tcgatgcggc catacgtccg cgcagcccta
    24481 aacgtcatct gggcatgatc cttggcatgg aggacgagat tccgcatcgt gacattctcg
    24541 gccatcagaa c
//
LOCUS       DAOSUT010000054        24416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1347_length_24416_cov_10.376462, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000054 DAOSUT010000000
VERSION     DAOSUT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_24416_cov_10.376462"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLD05_06760"
     CDS             <1..1158
                     /locus_tag="PLD05_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77172.1"
                     /translation="DQPPETLKSNPRFMAAMQVVKQRNVSLFLDLDVLRQLPELKSGL
                     AQSKEPIPVLLLGGAVAALEKAPWMALGIDIEQNRLSGTLRLGEISEWPSALSFAVAP
                     PDDGILPPLEVPNLLASMSWYRNLRDFYAQKNELFPERTSGLIFFENMMGIFFTGRNF
                     TEEVLAELEPHIRLVVAAQQFAPEVGTPTVQLPAVALVFRMKNPEKFAPVVEEGFQKA
                     LGLINFVRGQDAKPGLILDRPEYHGVKYTLAYFSAANEPDRSRLDIRFNFSPVLACRG
                     RDLILASAEPLAKDILDALAKEEGATAVKTVTGQIRVVGDQIARLLQLNLEALVTQNM
                     AEKGHGRQEAQAEITQLIQILQMFNRLDIKESLTGGQSQIDIGLEWTSPGQ"
     gene            1282..2577
                     /locus_tag="PLD05_06765"
     CDS             1282..2577
                     /locus_tag="PLD05_06765"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HOP77173.1"
                     /translation="MTMDQTITQPTSAGPFHVARCLRKALCFVDPAQKRRLSKTSPEE
                     VVSYILKGEATDNSLQELRLLKSQSAAAGNVGLYRTWRLRQMLESPHPFREMMTLFWL
                     DLFAISALRIKDLPCYHAFCEGLEERALAPWGEVLGYVLQQPAFLANLRAERNYRAMP
                     NRQLGEFIVKYLMGREPTQTQDTVTAVARSFTGLFVRGGELRRYEYEHDPAIKVIGRA
                     SGDFWPKDVAKLLAEDTDTAAVLADRLYRWFIASDGDIPPNFRDSVAKRILAGSAMGD
                     VTVEAIATWAGRGNTLARRIKSPLEVFLALARPFKGRATTALADLVAELGWDLLHPTT
                     YGGWPKGRDWMNTAQWARRISLAEGLLRKEDRFGGGVDLMAAAKSADYGDDWPNFLLD
                     ALLDDGVPPELRPAWKRHLQRGPGEALLFLVALPEFQLS"
     gene            2647..3900
                     /locus_tag="PLD05_06770"
     CDS             2647..3900
                     /locus_tag="PLD05_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOP77174.1"
                     /translation="MLNRRKFLAVSGGALSAGVASIGTISLATTEVAGDLGGDETILV
                     ALQMAGGDDGLNTVVPYRDDNYRRLRPTLALKESEVIRLQDDLGFHPALKPLMPLWEE
                     GHLAVVQGVGYPQASDDHGRAMEIWQTARPEDPLADTGWLGRAADELFEKGNTARAAA
                     PAVFLGQIPIPLTVVGRHQPAVRCQQPAELFSLHGGAASLPHYRQLIHEATDGRQTTT
                     HGLLAETVEVQRQTTEHLSRLEKVLGTSDDGKQFPQTNFGRQIACLTRLVRANLGIRI
                     FCLDLGGQGPGTFDTHALQAANHGELLAELAQGLALLADVLKKDGLFDSVAIYTYSEF
                     GRTIRENGRKGTDHGAAAPVFVLGGKVKGGLVGKHPPLDVPEKGGVPHHTDFRSLYAT
                     FLDRWLHVPSERVLGRSLGHLPLFA"
     gene            4035..5879
                     /locus_tag="PLD05_06775"
     CDS             4035..5879
                     /locus_tag="PLD05_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77175.1"
                     /translation="MFAGPIFTRELAICPRRKQFFIARTAYGAILLLLLCTAWLVVTG
                     TQYIRDVGDSARFGAAWFQIAAPLQVILAIFTSALVAAASICQEKDKRTFDLLLVTDL
                     RNTELVLGKLLSSLVTLFAAFVGGIPVYFFAGLLGGISYGQILRVTLVTVVALLGGGS
                     LGALIAFWREKTVQAITLTAMGVVFWLGFGELAAWIGRSEILWGLTGQTLAVAISPWR
                     AMLEAAKPLLVETPSLGVLRYDLSLFFVVNGAAVILMNAIAIAGVRHWNVVRDPHRII
                     PEELPDELSEDVKEELVSTSGAGEGTTTEADREQRRSRLARLHAAPGRAREVWDNPVL
                     WRELCTWAYGRKILIVRLVYILLFCLAAVGLHFVGETPDGLNSAAIAAISIPLLMLSL
                     VLVNTQAVTSLTTERDGRTLDTLLVTDISPREFIFGKLLGTFYNTKEVLVLSTLLCIW
                     FWWRRWLTLEDTIYLVVGLWVLFGFVAMLGVHAGLNYVNSRHAIATSLGTVFFLFVGV
                     AVCMRVMLAFSGSFQAQLHPFLAFMIGGGAGLYMALGVRNPSNAIALASFACPLATFY
                     ALTSLFLGQTHLVFVTIVAAYSFATAAMLIPALDEFDVATGRTSLDET"
     gene            5937..7280
                     /locus_tag="PLD05_06780"
     CDS             5937..7280
                     /locus_tag="PLD05_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOP77176.1"
                     /translation="MALVLSALPFLIVMGLLGVMSACFSAAEAAFFSLRKQDQDALRR
                     SGAAGQLVIGLLKDVEQLLTAILFCNLLVNIAYFAVSSIVTLTWQRMGATAEAGVFAS
                     VSVLSLILFSEMFPKSVAISWPQGVAQLLAVPIWIAVRLTQPWLPFFRSAAITIRRIL
                     WPRFRPEPYLRVRDLEQAFDFSRESGVVLEQEHQILQNLVQLSELKSEEVMRPRTEVP
                     IFHRPLQRDQLGQELPSSGYFLIVEDEDEAFTRALSPDQLLQALADQPEDGPSATKTD
                     SESCPGADQTAEDLAKWAEPVVYVPWCVSAAQVLDLLVEENRAVAAVVNEYGETVGII
                     TLDDLLRRIFAPESSRSESLYKRRSIQNVGPNVWHVTGLTSVRRLARYFGIDPPPGSS
                     LTVGGIFQEKLGRVAKQGDTCTWGPFVLKVLQMGRHGAVLVEVRPSQEGPKEVGE"
     gene            7277..8245
                     /locus_tag="PLD05_06785"
     CDS             7277..8245
                     /locus_tag="PLD05_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOP77177.1"
                     /translation="MIVLGLISFAVGLFLSALFSGSETGFYRVARLRLRVAAVTGDWI
                     SQAILWVSYHPSLFIATALVGNNLANYLLSFATVLLAQAFTASPQGWTEILATMIMAP
                     VAFVLGELLPKRLFLLAPFTLLRYTGPLFLVFTVVLAPVSVILWIWNRFLALLIPTPP
                     EIAQSQIRGWELRHIVEEGEHAGLMTNSQVRLANVILQAGTRPIKEWMHPLGELATVP
                     LDAPPEKNAELLCRGKRFLLVTHPQAAHRVVGYVDLAQWVLMPDRPLREVTRPFLRVL
                     PTERLVSVLQLMYQHGQKLALVIDGKDQAVGVVWEQELRRFCLTSS"
     gene            8635..8865
                     /locus_tag="PLD05_06790"
     CDS             8635..8865
                     /locus_tag="PLD05_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77178.1"
                     /translation="MGRHKVVPYAWLENLLATTTRDNVTVGASDYTIGAGRYGAGTGA
                     CPYRDGCNAGTSLRTPGSSNYHDKALSVISLA"
     gene            8889..10574
                     /locus_tag="PLD05_06795"
     CDS             8889..10574
                     /locus_tag="PLD05_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOP77179.1"
                     /translation="MTSVLPALLAMFPLLLVFVLLVGWQWPARRTMPLCYVATLIIGA
                     FYWQMTWSRILAATVEGLIIALMLLYIIFGALLLLAMLKHSGAVSAIRDGFRKISPDR
                     RIQAVIVGWAFGSFIEGASGFGTPAAVAGPLLVILGFPPLAAATATVTIQSTPVSFGA
                     VGTPILVGVNKGLQDQPTVLTYLAEQVSGQGLSPAAAFEQLLYSIGCRVAIIHGLIGL
                     LIPFFVVCLLTRFFGARRSWREGLEVWKFCLFSGLAFCVPYVTFAWVLGPKFPSLLGG
                     PVALILTTLAARAGWFQPKKPWDFAESPTENPGHRAVGEAVTASWNAGVWWAWLPYVV
                     VAGLLVLEQWNPIKNTLARANWEWTIAASEVTSQAIVARWNILASPGTAFFLAVLFTI
                     VLFAIPRESLQKAFGEAGQKTLAAALALVFAVPAVRIFIQSNVNGAGLASMPQELAAA
                     VSQCVGGVWPAFAAGIGALGAFIAGSNTISNMTFALFQFEVALRSSLDPLWIVALQAV
                     GGAAGNMVCVHNVVAAAATVGIIGQEGSIIRKTMIPTIYYLVAAGILGLAFCH"
     gene            11018..12592
                     /locus_tag="PLD05_06800"
     CDS             11018..12592
                     /locus_tag="PLD05_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HOP77180.1"
                     /translation="MRGAPIATGFLDRLRRWPALTLTLSQRERELWDRLLAATLLFWC
                     IACYTTCAEDSSPRSPLRWAADAEGGAPYIFQDPNNPQQVIGFEVDLAKALAEKLGRP
                     VEWVQYDYMSLVAGLQRGDFDFAMNGLEVTPDRAKVVRFSRPYYVYTLQLVVRANETR
                     FSNLDELLALGGTVGTLEETAAQRLLEGMGFQPKLYGNQTEPYLDLELGRIDAVLMDL
                     PIAVYFAKPNPKLKFLGEPLGEGYYAIAFRPEDEELAVAVDQALTELIAEGQIRAIYE
                     KWGLWNTAQEKLLQAEIIDVIAQARQKWTLRRYFPLLLRGAWVTTYLSVLSMLLAMIL
                     GLPIALARLFGSLPIRWLAVGYVEFFRGIPILLLLFFLYYGLPTIADQYGLPFTFRLS
                     PITAAILGFGLNYAAYEAEIYRAGIRSIPSGQWEAAASLGMGRVLTFRRIILPQAIRV
                     ILPPMTNDFIALFKDTSIVSIVAVVELTKQYQILAKSSLKYLEIGLVTALLYLILSVP
                     LGALSRHLEARWGKGQ"
     gene            12589..13347
                     /locus_tag="PLD05_06805"
     CDS             12589..13347
                     /locus_tag="PLD05_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOP77181.1"
                     /translation="MNKDEFSIEVEDLCKRFGHRTVLHKVAFRVDRGETVAIIGPSGA
                     GKSTLLRCLNGLCNFDGGTIRVGSLVLRSGRRPAPFATLRQIRQRLGMIFQDFQLFPH
                     LTVLQNVTEAPIHVLGVARRDAERQAQALLDRVGLADHIHAFPQQLSGGQKQRVAIAR
                     ALAMNPFGLLCDEITSALDPELKAEVLAVLEDLRRDGMTLILVTHEMAFAQRVADRII
                     VLVDGEIVEDGPPARVFRTPEHPRTTRFLEQVLL"
     gene            13367..14503
                     /locus_tag="PLD05_06810"
     CDS             13367..14503
                     /locus_tag="PLD05_06810"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOP77182.1"
                     /translation="MRKNSTRAVRVGSVTFGGGNPIVVQSMCATKTQDVAATVAQVEQ
                     LRQAGAGIVRIAVDSPADAQALVAIREQTSANLSVDLQENYRLVTQVAPYVNKVRYNP
                     GHLYHHQTNVPWQDKVKFIADVAAQYGCALRIGVNCGSIDPAKREKFPSEDKISPMLE
                     SAWEHCELLDRLGFSQYCVSLKESDPQMVIEANRRFAAARPDVPLHLGVTEAGMPPEG
                     IRKTRAALIPLLAEGIGDTIRVSLTLPNERKHEEVLAGLEIVADVAAGRVTVELWTRD
                     GLNIVSCPSCSRVENAAFVQLTEKVRELTAYAASYPITIAVMGCRVNGPGETDDADLG
                     LWCGPKYVNLKKGPVTLGSFTYDEILPRLKQELDALIAERFPVT"
     gene            14517..14738
                     /locus_tag="PLD05_06815"
     CDS             14517..14738
                     /locus_tag="PLD05_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77183.1"
                     /translation="MTVCLFTDFAYVPRWCSLAIVVRPFLCNRGADTDGDITPTEGGL
                     PYGPVEVTIIGGDRDEGYVTIKVFGPFGR"
     gene            complement(15238..17184)
                     /locus_tag="PLD05_06820"
     CDS             complement(15238..17184)
                     /locus_tag="PLD05_06820"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOP77184.1"
                     /translation="MSRKTFILCRQNAGNLVAIMAVVLLGMGMGDRATAEPIAGGVSA
                     ERAAYWAKHLPPKPVGVGAPISQREIWDVLAKDKAFRQVVAEAEEELSRPIAEVPDEL
                     YLDFSRTGNRRRYESRMSARRGRLGNLALAECLENRGRFLAELEKTIAAICQEKTWVM
                     PAHDGRLDNFYGRTVEIDLGAASTAWNMATIDYWLAEKLSPECRRLIASELDRRIFTP
                     FEQAVNTGNPRLWWITGTNNWNAVCLAGVVGAALENIPSPERRGFFVAAAEKYIQYFL
                     QGFTPDGYCSEGIGYWNYGFGHFTLLGETLVQATGGQLDFFALPGVPQIALFGYRMEV
                     APNIYPAFADCSVGSRPDSVLSAYLSRRFDLGLKELENRYLGVGAGPSSFLPEVAIYA
                     MPNSLTNRTGQAAARRHDIRDWFADAGILICRPTDLSPDKLAVALKGGHNAEHHNHND
                     VGSFVVALAGQTPLLDPGAEVYTARTFSGRRYESKVLNSFGHPVPVVAGQLQRTGSQA
                     RAKVLETQFSNEADVYRMDITSAYNVPALKSLVREFRYERQGRGRLIVTDRVEMSSPQ
                     SFEEALITYSPWRRVGNGVWIVGEGRGAVRVELTMEGQSFVASEEEIDEDVRYNKKPV
                     RLAAKVKEPIASGTVVIRIEPVEK"
     gene            complement(17260..18609)
                     /locus_tag="PLD05_06825"
     CDS             complement(17260..18609)
                     /locus_tag="PLD05_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOP77185.1"
                     /translation="MAEKLDDYLILISVANLREKDVARMENLKEMMAGGEIVDLIPSF
                     PALTCPVQANMTTGRPPKRHGIIANGIYWRDRDEVEMWTSPNDCIEVPQLWDLLYHHE
                     RGLTSAIWFPQHAKEAGADFICTPAPIHNPDGSETMWCYTRPENLYGELRQKLGDFPL
                     HRYWGPLANIESTRWIVESAIELAKAARPNLFYIYLPHLDYAAQKDGPDSVAAGKAVL
                     ELDEVLGQLMNGLRDAYDGDPCWLIAGEYAITPVERVIYPNRILREAGLLSVREERDG
                     EYLDTTSAAWALADHQIAHVFVRDGEPEIIDRVMRLFQGLPGIDEVLAGEQRSKYHMD
                     HPRSGEVILTAQPDTWFAYYYWFEDHKAPPFARKVDIHRKPGYDPVELFFDPATRGIP
                     LKPELVHGSHGAPVRERFQRTVAISSRPGVFPREPVTDTDIFHVVLKHFGMTMDGLA"
     gene            complement(18714..19577)
                     /locus_tag="PLD05_06830"
     CDS             complement(18714..19577)
                     /locus_tag="PLD05_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77186.1"
                     /translation="MRKSERLMILGYNTNGLVHHEPTAALRGLAKIGYRGVALTIDHY
                     LLSPFNLYWERQLHELRDVLDRLGMYCVIETGARFLLDPWHKHEPSLISPDASGRVRR
                     LDFYRHALRCAVTLHADCLSLWSGNRHDEVPPEQAWEWLIQGLSHLCDEASAMGVPVA
                     LEPEPGMFIATTADYDELCRRAPSLGCLLTLDIGHVLCQEEGPISSIVEQYAPRIVNI
                     HLEDMRRGTHEHLMFGEGEVDLAEVFASLQKIGYKKGIYVELSRHSHLGLQAAKQAFD
                     ILSRYQSGMTQ"
     gene            19763..20608
                     /locus_tag="PLD05_06835"
     CDS             19763..20608
                     /locus_tag="PLD05_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOP77187.1"
                     /translation="MRRWKTVSIVGVGLIGASIGQAVLRRGLADEVIGIARRQTTLRA
                     ARRVGAVSHTTIDLDRGVAQADLIIVCTPVGTIPDLVRRVAEACPERALISDVGSAKG
                     QIVAALDEGLPRGCRFLGAHPLAGSEKTGPSYSDPNLFEGRVTIITPTKNTRAEDFDA
                     LEDFWTGLGSVVIRMSPEEHDKALAMTSHFPHLVASLLAGMLPEPWFRLTGSGFFDTT
                     RIASGDVEMWKQICMQNRENLVGACQAFADELGRLARALAAGDESTLEQILLRGKKNR
                     DALGS"
     gene            20592..23498
                     /gene="purL"
                     /locus_tag="PLD05_06840"
     CDS             20592..23498
                     /gene="purL"
                     /locus_tag="PLD05_06840"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOP77188.1"
                     /translation="MLWEVDLFPAEGQPDVAARQIRQDAHDLGIHKDWTILTCHGYLI
                     EGQLTEAQVRQVTEELLADPVVERSLVAPVGDWSLTAPPQPGMEVIYVLPKPGVMDPV
                     AQSAQSAIADFGWKADQVRTFRKYWVAGLSQAEIKRLCQKILANDAIEQVVLGPLPFR
                     TLEQGRPYEFRLITVPIRDMDDEALVRLSREGQLFLSLVEMQTIREYFRQQGRDPTDV
                     ELETLAQTWSEHCSHKTLAGRIRYQDDQRQIEFNNMLKETIFAATHEIRKSLGQDDWC
                     VSVFEDNAGVVRFDERYHVCFKVETHNHPSALEPYGGANTGIGGVIRDPMGTGLGAKP
                     ICSTDVFCFAPPDVSHDELPPGVLHPQRVMKGVVAGVRDYGNRMGIPTVNGAVYFDKR
                     YLGNPLVYCGNVGLLPCEKARKMPQPGDLIVAVGGRTGRDGIHGATFSSAELTTESET
                     LSGGAVQIGNAITEKMLLDVLLEARDRGLYNAVTDCGAGGFSSAVGEMGRDIGAEVEL
                     SHVPLKYEGLSYTEIWISEAQERMVLAVPEKHWPELKALFESEGVEATVIGRFVPTGR
                     LVLRYAGHEVGNLSMEFLHHGRPRVVREAFYRPPQVRPLIIKDNAEATAGEDLLAILR
                     SPNVCSKHWIIRQYDHEVQGGSVIKPLVGRWDDGPSDAAVLRPVLDSYRGLVIACGMN
                     PRYGDLDPYWMAASAIDEAIRNCVAVGADPRRIAILDNFCWGNTDRPETLGSLVRAAL
                     GCRDMAVVLQTPFISGKDSLNNEFRVSETETISIPPSLLISAMGQIDDVRRCVTMDLK
                     QPHNVIYLVGMTADEMGGSHWCLVRHQEGGRVPHCDPHRAKRIFSALHAAIEAGCVRA
                     CHDLSEGGLAVAAAEMAFAGCWGMELDLDHVPCETSSLAPIIRLFSESNTRFLCEVPP
                     ERQSHFESYFAGLPVAKIGMVTEDDRFVVRFAGRTLIDLPLRTLKEAWLSPLAW"
     gene            23568..>24416
                     /locus_tag="PLD05_06845"
     CDS             23568..>24416
                     /locus_tag="PLD05_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOP77189.1"
                     /translation="MAKPRVLILRAPGTNCDIETAYAFEKAGAVTDRIHIRRLLEQPA
                     TIRSYQILCVPGGFSYGDDVAAGKILAVQLRHHLLHVLEEFHQADKLILGICNGFQVL
                     LKTGLIVPPDESGKPWATLTWNESARYEDRWVHLEVASDRCVFLRGIERLQLPIAHAE
                     GRFVVRQASGLDRLAQAGQIALKYALPMELKRMARQENAASRQSVEAAQMATRCLGGA
                     SLSALESAVAKANDQRLPYPWNPNGSMGNIAGVCDRTGRVLGLMPHPERFIDPTHHPR
                     WTRGEWH"
BASE COUNT         4746 a         7020 c         7281 g         5369 t
ORIGIN      
        1 gatcagccgc cggagacgct taagagtaac ccaagattca tggcggccat gcaggtcgtc
       61 aaacagagaa acgtgagtct ctttctcgat ttggatgtgc tccgacagtt gccggagctg
      121 aaatcgggcc tcgcccaaag caaggaaccg attcctgtcc ttctccttgg aggggccgtg
      181 gcggccttgg aaaaggcccc ctggatggca ctcggcatcg atatcgaaca gaaccggctc
      241 agtgggaccc tccggctggg tgagattagc gagtggccca gcgcgctctc cttcgccgtg
      301 gccccacccg atgacggaat cttgccgccg cttgaggtgc ccaacctcct ggccagcatg
      361 agctggtacc gcaatctccg cgatttctac gcccagaaaa atgaactctt cccggaacgg
      421 acgtcagggt tgatcttctt tgaaaacatg atggggatct tctttaccgg ccgcaatttc
      481 accgaggaag tgttggcaga gctggaaccc cacatccgcc tggtcgtggc cgcccaacaa
      541 tttgccccgg aggtaggaac accgactgtc cagcttcccg cggtggcttt ggtcttccgc
      601 atgaagaatc ccgagaagtt cgccccagta gtggaggagg gtttccaaaa agcgcttgga
      661 ctgatcaact ttgtgcgcgg acaggacgcc aaacccggtt tgattctcga tcgcccggaa
      721 taccacggcg tgaaatacac gctggcctat ttctctgcgg ccaatgagcc ggaccgctcg
      781 cggttggaca tccgctttaa tttcagtcct gtcttggcct gccgaggacg cgacctgatc
      841 ctcgccagcg cggaaccgct ggccaaggac atcctcgatg ccctggcaaa ggaagaaggg
      901 gcgactgcgg tgaagaccgt caccggccag attcgggttg tgggagacca gatcgcccgc
      961 cttttgcagc tcaatctcga ggccctcgtc acccagaata tggccgaaaa agggcatgga
     1021 agacaagaag cccaggccga gatcacgcag ttgatccaaa ttcttcagat gtttaatcgc
     1081 ctagacatca aggaaagcct aacaggtggt caatcgcaga tcgacatcgg cctcgagtgg
     1141 acttcgcccg gacaataaac gcggtgccgt aatcacgcgc tatttggctg ccactagccg
     1201 tctggcaaaa aggccaaagc gggaggttaa ggcaatattg atctcggacc gccgtccctg
     1261 ccgcaagaag ggattatgcc catgaccatg gaccaaacga tcactcagcc cacatccgca
     1321 gggccattcc atgtggcacg ctgtcttcgc aaggccctct gcttcgtgga ccccgcccag
     1381 aagcgacgcc tgagcaagac ctccccggag gaggtcgtct cgtacatcct caaaggtgag
     1441 gccaccgaca actcgttaca agagcttcgc ctgctgaagt cccaatccgc ggcggcagga
     1501 aacgtgggac tttatcgcac ttggcggctg cgccagatgc tcgaatcgcc ccatcccttc
     1561 cgcgaaatga tgaccctctt ttggctcgat ctgtttgcga tctcggccct tcgcatcaaa
     1621 gacttgccat gttatcacgc cttttgtgag ggtctggaag aacgggcctt ggctccgtgg
     1681 ggcgaagtct tgggctatgt cctgcagcag cccgcttttt tggccaatct tcgcgcggaa
     1741 aggaattatc gcgcgatgcc gaaccgccaa ctgggcgagt tcatcgtcaa gtacctcatg
     1801 ggtcgtgagc caacacaaac ccaagacaca gttaccgctg tggcccgttc attcaccgga
     1861 ttgtttgtcc gcggtggtga gcttcgccgt tatgagtatg aacacgaccc agctatcaag
     1921 gtgattggcc gggcctccgg cgatttctgg ccgaaggatg tggcgaagct tcttgccgaa
     1981 gacaccgaca cagccgccgt cttggccgac cgcctttatc gttggttcat cgcctcggat
     2041 ggggacatcc ctccgaattt tcgcgacagt gttgccaagc ggatccttgc cggtagtgcg
     2101 atgggcgatg taacggtgga ggctatcgcg acgtgggccg gtcggggcaa cacactagcc
     2161 cggcggatca agagtccgct cgaggttttc ctggccctgg cgcgcccgtt caagggacgt
     2221 gccaccactg cgctggccga tttggttgcg gaattgggtt gggacttgct tcatcccacg
     2281 acgtacgggg gctggcctaa ggggcgggat tggatgaaca cggcacaatg ggcgcgacgg
     2341 atcagcctgg cggaagggct tttacgcaaa gaggaccgct ttggtggcgg tgtcgatctg
     2401 atggcggcgg caaagtcggc cgactacggc gacgattggc ccaacttctt actagatgcc
     2461 cttctggatg acggagtgcc accggaattg cgcccggcgt ggaaacggca tctgcaacgc
     2521 gggcccgggg aagccctcct cttcctcgtc gccttgccgg agttccagtt gtcttgatcg
     2581 taaaggagaa gaatcgcgcc gcggcatcac acccaatcac gaccgtccca aaaggagaaa
     2641 gtgcccatgt tgaaccgtcg caagttcctg gccgtttccg gcggagcctt aagtgccggc
     2701 gtcgcttcca ttggaacaat ttctctggcg acaaccgagg ttgctggaga cctcggcggg
     2761 gacgagacca ttctggtggc cctacagatg gctggggggg atgatggtct caacacggtg
     2821 gtgccctatc gggatgataa ctaccgccgt ctgcggccaa cattggccct gaaggaatcg
     2881 gaggtgattc gccttcagga cgatttgggg tttcatccgg ccctcaagcc tttgatgccg
     2941 ctatgggagg agggccatct cgcggtggtc cagggggttg gttatcccca ggcttcggac
     3001 gatcacggcc gggcaatgga gatttggcag accgcccgac ccgaagaccc gcttgccgac
     3061 accggctggc tcggtcgtgc cgcggatgag ctttttgaga agggtaacac cgcccgcgcg
     3121 gcagcccctg ccgtgtttct cggccagatt ccaatcccgt tgaccgtcgt gggaagacac
     3181 cagcctgcgg tccgctgcca gcaaccggcg gagctgtttt ccctccacgg gggcgccgct
     3241 tcgctgcccc attaccggca gttaatccac gaggccaccg acggtcggca gaccacgaca
     3301 catggccttc tggccgaaac ggttgaggtt cagcggcaga ccacagaaca tctctcgcgg
     3361 cttgagaagg tcttgggcac ttccgacgac gggaagcaat ttccccaaac gaacttcggc
     3421 cgacagatcg cttgtctaac gcggctggtg cgggcgaact taggcattcg catcttttgc
     3481 ctggacttgg gcgggcaggg gccggggact ttcgacacac acgcgcttca ggcggccaat
     3541 catggggaac tgctggcgga actcgcccag gggttggccc tgcttgccga cgttctcaaa
     3601 aaagatggcc tctttgatag tgtggcgatt tacacctact cggagttcgg gcgaaccatc
     3661 cgcgagaatg gccgaaaggg gaccgatcac ggtgccgccg cccctgtttt cgttctaggt
     3721 ggcaaggtga aaggcggttt ggtgggaaag cacccgccgc tagatgtacc cgaaaaaggg
     3781 ggcgtgccac atcacaccga tttccgcagc ctttatgcga cgtttctcga ccggtggctt
     3841 catgtgccca gtgaacgtgt cctggggcga agtttgggac accttcccct tttcgcgtga
     3901 tgttgggcgg tggaggccgt cgcgccgtcg tggccgggcc ggcaaaaagg ccaatctctt
     3961 tcagattcgg cgtcggcaat ctatgatggg aagaaacgtc catttgctgt gagagaacct
     4021 taacggagag ccccttgttt gccggtccca tttttacccg tgagttggcc atttgtccgc
     4081 gccgcaagca atttttcatc gcgcggacgg cttatggtgc gattttgttg ctccttttgt
     4141 gcacggcctg gctcgtcgtc acagggaccc aatacattcg cgatgtgggg gattctgccc
     4201 gatttggcgc ggcatggttt cagattgcgg ccccgctcca agtgattctg gcgattttta
     4261 cttccgccct agtggccgcg gccagtattt gtcaggagaa agacaaacgg acattcgatc
     4321 tgctcctggt gaccgatcta cggaatacgg agctcgtgct cgggaagctc ctaagtagcc
     4381 tggtgaccct gtttgccgcc tttgtggggg gtattcccgt ctatttcttt gccgggcttt
     4441 tggggggaat ttcttacggg cagattctgc gggtgacact cgtcaccgtg gtggccctgc
     4501 ttggtggggg aagccttggg gccctgatcg ccttctggcg ggagaaaacc gtccaagcga
     4561 tcaccctgac cgcgatgggt gtcgtcttct ggttaggatt tggtgaactg gccgcgtgga
     4621 tcgggcgatc tgaaatcctt tggggactga cgggccaaac cctcgctgtg gcgatcagtc
     4681 cctggcgggc catgctcgag gcggctaagc cactgctcgt ggagacgcct tcgctgggcg
     4741 tcttgcggta cgatctgtca ctcttcttcg tcgtgaatgg ggccgctgtc atcctgatga
     4801 acgccattgc cattgcgggc gtgcgacact ggaatgtggt gcgagatccc caccggatca
     4861 tcccggaaga gttgcctgac gagctaagcg aggacgtcaa agaagaattg gtttcaacca
     4921 gtggagcggg agaagggacc accacggagg cggatcgcga gcagcgcagg tctcgcctgg
     4981 cccgactgca cgctgcccct ggtcgggccc gggaagtctg ggacaatccg gtactctggc
     5041 gagaactgtg cacctgggcg tatgggcgaa agattcttat cgtgcggctg gtgtatatcc
     5101 ttttgttctg cttggcggct gtaggattac actttgttgg cgaaaccccc gacggtttga
     5161 attctgcggc aattgccgcc attagcatcc ccttgctcat gctcagcttg gtcttggtca
     5221 atacccaggc cgtcacttcg cttactaccg agcgcgatgg gcgaacgctg gacaccctgc
     5281 tggtcaccga catttcaccg cgggaattca tttttggaaa acttctggga accttctaca
     5341 acaccaagga agtccttgtc ctttccacct tgctgtgtat ttggttctgg tggcggcgct
     5401 ggctgacgct ggaggacacg atctaccttg tcgtgggctt gtgggtcttg tttgggtttg
     5461 tggccatgct gggtgttcat gcgggcctca attacgttaa ttcccgacat gccattgcca
     5521 cgagtttggg tacggtgttt tttctgtttg tgggcgtggc cgtgtgcatg cgggtgatgc
     5581 tggccttcag cggttcgttc caggcccaac ttcatccctt cttggccttc atgattggtg
     5641 gtggggcggg gctatacatg gccttgggtg tccgcaatcc ctcaaatgcc atcgccttag
     5701 cttcgtttgc ctgtcctttg gcgacttttt atgccttgac cagtctgttc cttgggcaaa
     5761 cgcatttggt ctttgtgacg attgtggcgg cctacagttt cgccacggcg gccatgctga
     5821 tccccgccct ggatgaattc gatgttgcca ccggccgaac gtcgttggac gaaacatgac
     5881 catggaagtg atggcggaaa cgacatcgtc ccttggttga ggagtcatac gagatcgtgg
     5941 cgctggtgct atcggccttg ccgtttctga ttgtgatggg gctgcttgga gtgatgtcag
     6001 cctgcttctc cgcggcggag gctgccttct tttcgctccg caaacaggat caggacgcac
     6061 tccgacgaag tggcgccgcc ggtcagcttg tgattgggct actaaaggat gtggaacagc
     6121 ttttgacggc catcctcttt tgtaatctcc tcgtcaatat tgcctatttt gccgtttcct
     6181 ccattgtcac gttgacctgg cagcggatgg gagccacggc cgaggccggt gtcttcgcaa
     6241 gtgtctcggt gttgtcgctg atccttttca gcgaaatgtt tcccaaaagc gtcgccatct
     6301 cttggccgca gggcgtcgcc cagctcctgg ccgtccccat ctggatcgcc gttcgcctga
     6361 cgcagccttg gcttcccttc tttcgatcgg ctgccatcac gattcgccgc atcctttggc
     6421 cgcgttttcg accggagcct tatcttcgcg ttcgcgacct ggagcaagcc tttgactttt
     6481 cacgcgaaag tggtgtggtc ctcgagcagg aacatcaaat cctgcaaaat cttgtgcagc
     6541 tctccgagct gaaatcggaa gaggtcatgc gaccgcgaac ggaggtgccc atctttcacc
     6601 gtcctttgca gcgagatcaa cttggccagg agctaccttc cagtggctac ttcttgatcg
     6661 ttgaagacga ggacgaggcc tttacccggg cgctctcccc tgaccaattg cttcaggcat
     6721 tggcggatca gcctgaggat ggtccctcgg cgacaaaaac ggactctgaa tcgtgtcccg
     6781 gtgccgacca gacggcagag gacctcgcga aatgggcgga acccgtcgtg tacgtcccgt
     6841 ggtgcgtttc cgccgcccaa gtcttggacc tcttggtcga ggagaatcgg gcggttgcgg
     6901 cggtcgtcaa tgaatatggg gaaacggtgg ggattatcac gctggatgac cttttgcggc
     6961 ggatttttgc cccggaaagc agtcgcagcg agtcgcttta caagcgacgt tccatccaaa
     7021 atgtggggcc aaacgtctgg catgtgacgg gcctgacgag tgtgcggcgg ttggcccgct
     7081 actttggcat cgatcccccg cccggctcga gcctgacggt tggtgggatc tttcaggaaa
     7141 aactgggcag ggtggccaag cagggtgaca catgcacctg gggacccttc gtccttaagg
     7201 ttctacaaat gggtcgccac ggggctgtgc tggtagaagt caggccgtcc caggagggac
     7261 cgaaggaggt cggcgaatga tcgtcctggg acttatctcc tttgccgtgg gattgttcct
     7321 cagtgcccta tttagcggtt ccgagacggg tttttaccgt gtagcccggc tccgccttcg
     7381 ggtggccgcc gtcacgggtg actggatttc ccaagctatt ctctgggttt cgtaccatcc
     7441 ctccctcttt attgccacgg ccttggtggg gaataacctg gccaattacc tcttatcgtt
     7501 tgccacagtg ttgctggccc aggccttcac tgcaagtccc cagggatgga cggaaatcct
     7561 ggcaaccatg atcatggccc cggtggcttt tgtgttgggg gaactgctcc cgaagcggct
     7621 ctttcttttg gccccattca cactgcttcg ttacaccggg ccgctgttct tggtctttac
     7681 cgttgtcctg gcaccggttt cggtgatctt gtggatctgg aatcgcttcc tggccctttt
     7741 gatccccacg ccgcctgaaa tcgcccaatc gcagatcagg ggctgggaat tgcgccacat
     7801 tgtggaagaa ggcgaacacg ccgggctgat gaccaatagc caggttcgtc ttgccaatgt
     7861 aatcctgcaa gcggggacgc ggccaatcaa ggagtggatg catccgcttg gcgaattggc
     7921 aactgtaccg ctcgacgcac caccggaaaa gaacgcggag cttctctgtc ggggcaaacg
     7981 ctttctgctc gttacccatc cccaggcggc ccatcgcgtg gtgggctacg tggatctcgc
     8041 ccagtgggtg ttgatgcccg atcgtcccct gcgcgaggtg acgcgacctt ttttgcgagt
     8101 ccttcccacc gagcgactgg tgagcgtgtt gcaattgatg tatcaacacg gtcaaaaatt
     8161 ggcccttgtg atcgacggca aggaccaagc ggtgggagtc gtttgggaac aagagttgcg
     8221 ccgtttctgc ctgacttcgt catgacctgt gccgattgtc atcccgagcc gccggcgtaa
     8281 gcgggtgatg gttttcgata gaccatgcgt catctcgtct gcctggtcct atcgctgtgc
     8341 gtattccaat ccttgttggg gatacccaat gggtagcgcc cataacccta tagattggcg
     8401 cggccacgag gggccatggt cctccatcgc tggcaggcgc aacccttgtg gtcgctcttt
     8461 tttgcttttg agccaatccc ataatctccc ctcgcccgtt cggcgggaga ggggcggggg
     8521 tgagggtagc tcgaatcgct gccggttttc tcgatcaact ccgatgattg cttgccctca
     8581 ccccaaccct ctcccggagg gagaggggga ctttttggca tagactcttc acgaatgggc
     8641 aggcacaagg tcgtccccta cgcatggctg gaaaacctgc tggcgacaac aacacgcgac
     8701 aacgtcacgg tgggggctag cgattacacg attggggctg gacgttatgg ggcaggcaca
     8761 ggggcctgcc cctaccggga tggctgcaac gcggggacct cgttgagaac gcccggttcg
     8821 tcgaattatc atgataaggc cctcagcgtc atatcgcttg catgattctt ggtcttttgg
     8881 aagaggtgat gacgagcgtg ttacctgcac ttttggcgat gtttcccctg cttctggttt
     8941 ttgtgctgct cgtgggttgg caatggccgg cccggcgtac catgccgctg tgctatgtcg
     9001 cgacactcat catcggggcg ttttattggc agatgacctg gtcgcggatt ctggcggcca
     9061 cggtggaggg cctaattatt gccctgatgc tcctttatat catctttggg gccttgctgt
     9121 tgctggccat gctgaagcat tcgggggccg tctcggccat tcgcgatggc tttcggaaaa
     9181 tctctccaga tcgtcgcatt caagcggtga ttgtcggttg ggcattcggt agttttattg
     9241 aaggggcctc cggatttggc acaccggcgg ctgtggccgg tcccctcttg gtgatcctcg
     9301 ggtttccgcc cttggcggcc gcaacggcca cggtcacgat ccaaagcacg ccggtttcct
     9361 ttggtgcggt gggcactccc atcctcgttg gtgtcaacaa gggcctgcag gaccaaccga
     9421 ccgtgcttac ctacctcgcc gagcaagtgt cgggccaagg cctgtccccc gccgcggctt
     9481 ttgagcagtt gctctacagc attgggtgcc gcgttgccat cattcacggg ctgattgggc
     9541 tcctcatccc gttcttcgtg gtctgcctgt tgacccgctt ttttggggcc cgtcgctcat
     9601 ggcgggaagg ccttgaggtg tggaagttct gcctcttttc cggtctggcg ttttgcgttc
     9661 cgtatgtgac ctttgcctgg gtcctaggcc ccaaatttcc ttcgctcctg ggaggaccgg
     9721 tcgccctcat cttgaccacc ctggccgccc gcgccggctg gttccaaccc aagaaaccat
     9781 gggattttgc cgaaagcccg acggagaatc cggggcatcg ggccgtgggt gaggccgtca
     9841 cggcgtcgtg gaacgcgggc gtttggtggg catggcttcc ctatgttgtt gtggcgggac
     9901 tcctggtctt ggaacagtgg aatccgataa aaaacaccct tgcgcgggcc aactgggaat
     9961 ggacaattgc cgcctcagaa gtcaccagcc aggcgatcgt ggcacgctgg aatatcctcg
    10021 catcgccggg gacggccttt ttcctcgccg ttctgttcac catcgtgctg ttcgccattc
    10081 cccgcgaaag cttacaaaaa gcgtttggag aagccgggca aaagacgctt gctgccgcac
    10141 tggccctggt gtttgctgtc cctgccgtgc gaatttttat ccagtcaaat gttaacgggg
    10201 caggattggc cagcatgccg caagagctgg ccgccgctgt gtctcaatgc gtcggagggg
    10261 tttggccagc ctttgccgca gggatcgggg cactcggtgc tttcatcgcc gggagcaaca
    10321 ccatcagcaa catgacgttt gccctctttc aatttgaagt cgccctgcga agcagcttgg
    10381 accccctttg gattgtggcc cttcaagccg tgggcggggc cgccggaaat atggtgtgcg
    10441 tccacaacgt ggtggctgct gcggccacgg tggggatcat cggccaggag gggagcatca
    10501 tccggaaaac catgattcca accatctact atctcgtagc tgccggcatc ttgggactcg
    10561 ctttttgcca ctgaccccta gggagggacg cgctcactgg cgtcaaacgt gttttttcac
    10621 gcgggaggga cgccgtcccc ggcgtcctgt cgtttgggag ggacgccgtc ctcgcgtcct
    10681 gccgtatgcg agttacgcgg tcccccgcgt ccgacattct ggaaaaacgc attttttcgc
    10741 gtccaccatg tcactcaagg caacttgcga gaagacgtac cgacaggata tccttgggac
    10801 aatgtggggt ttctcgagcg tctaaagaga accgtcggcc gctgactttg tcctggaaca
    10861 ccacgcggac cgtgcaaatt tcgcaaacaa ccgcagtcgg ttcatgtctt cgaaatcaag
    10921 attgagcgat cggataacgt cctgggttat acgcgttggg actttagagc ctatccctca
    10981 atcacccctc tcccgttcgc cgggagaggg accgggggtg aggggggctc caatcgccac
    11041 tggttttctc gatcgactcc gacgatggcc tgccctcacc ctaaccctct cccagcggga
    11101 gagggaactt tgggataggc tcttagcggc gactttgctt ttttggtgta tcgcctgcta
    11161 cacaacatgt gccgaggact catcacctcg gtcgccgctg cgctgggcgg cggatgctga
    11221 aggcggtgcc ccgtatatct ttcaggaccc caataatccg cagcaggtga tcggttttga
    11281 ggtcgatctg gcgaaggccc ttgcggaaaa acttggccgt cccgtggagt gggttcagta
    11341 cgactatatg agtcttgtgg cgggtcttca gcggggcgac tttgattttg ccatgaacgg
    11401 tctggaggtg acgccagatc gggcaaaagt tgtgaggttc tcgcgaccgt actatgttta
    11461 tacccttcag cttgtggtca gggctaatga aacccgcttc agcaatttgg acgaacttct
    11521 cgccctgggc ggaacggtcg gcacgctgga ggagacggcg gcccagcgcc tcctggaggg
    11581 gatgggcttc caacccaagc tgtacggcaa tcagacggag ccttatctcg acctggaatt
    11641 gggacggatt gatgccgttc tcatggatct gccgattgcc gtctatttcg ctaagccaaa
    11701 tcctaagctc aagttcctcg gggagcccct gggggaagga tattatgcga ttgccttccg
    11761 tcccgaagat gaagagctcg cggtggcggt cgatcaggcc ctgacggaat taatcgcgga
    11821 gggtcaaata cgggcgatct acgagaaatg gggcctttgg aacaccgctc aggaaaagct
    11881 gctccaagcg gagatcattg acgtcattgc ccaggcccgc caaaagtgga cgttgaggcg
    11941 atacttcccg ctcttgttgc gcggagcctg ggtcacaacc tacctgtcgg tgctgagcat
    12001 gctgctagcc atgatcctgg gcctgccgat cgccctggcc cgacttttcg gttcattacc
    12061 gatccgctgg ctggccgtcg ggtatgtgga gtttttccgc ggcattccca tcttgctcct
    12121 gctgttcttc ttgtactacg gactgcccac gattgccgac cagtacgggc taccgtttac
    12181 ctttcgtctg tcacccatca cggcggccat tttgggattt gggcttaatt acgccgctta
    12241 cgaggccgag atttatcgtg caggaattcg gtccatccct tcgggacagt gggaggcggc
    12301 ggcctcgctg gggatggggc gcgtgttaac cttccgccgg atcatccttc cccaagcaat
    12361 tcgcgtgatt ctccccccga tgacgaacga ctttattgcg ctttttaagg atacgagcat
    12421 cgtcagcatt gtcgccgtag tagaattaac caagcagtat caaattttag cgaaatccag
    12481 tctaaaatat ttggagatcg gcttggtgac agccctgctg tacctgatcc tttcggttcc
    12541 gttgggtgct ctttcccggc atctcgaagc tcggtgggga aaaggccagt gaataaagac
    12601 gagttcagca tcgaagttga agacctgtgc aagcggttcg gtcatcggac ggttttgcac
    12661 aaggtggcgt ttcgcgtgga tcggggcgaa acggtggcca tcatcggccc aagtggggct
    12721 gggaaatcga ccctcctacg ttgcctcaac ggcttgtgca attttgacgg cgggacaatc
    12781 cgcgtgggct cgcttgtttt gcgttccggg cgccgaccgg cccccttcgc tacgcttcgt
    12841 cagattcgcc agcgcctggg catgatcttt caggatttcc agttgtttcc ccacctgacg
    12901 gtcctccaga atgtcaccga ggcccccatt catgtgctgg gcgtggcccg gcgggatgca
    12961 gagcggcaag ctcaggccct cctcgaccgc gtggggttgg cggatcatat tcatgccttt
    13021 ccgcaacagc tttccggggg acaaaagcag cgggtagcca ttgcccgcgc cttggcgatg
    13081 aatccctttg gcctgctctg tgacgagatc accagcgccc tcgatccgga acttaaggcg
    13141 gaggtcctgg cggtcctcga ggaccttcgc cgcgatggaa tgacgctcat tttggtaaca
    13201 cacgaaatgg cgtttgccca gcgggttgcc gatcgtatca tcgttctggt tgacggcgag
    13261 attgtggaag atggtccacc ggctcgcgtc ttcaggactc ccgaacatcc ccgcacaact
    13321 cgctttctgg aacaggtgtt gctttagccc cgtgtgaaag gtctctatgc ggaagaattc
    13381 gacccgcgcc gtgcgtgtcg gctctgtgac gttcggcggc ggaaatccga tcgtggttca
    13441 gagtatgtgt gccacgaaga cgcaagatgt tgcggcgaca gtggcccagg ttgaacaatt
    13501 gcgccaggcg ggcgccggta ttgtgcggat cgccgtggat agcccggcgg atgcgcaggc
    13561 cttggtggcc attcgggagc agacctcggc caacctgtcc gtggacctac aagagaatta
    13621 ccgtctcgtc acccaggtgg caccgtatgt caataaggtc cgctacaatc cgggccacct
    13681 ctatcatcac caaacgaatg tcccgtggca ggataaggtg aaattcatcg ccgatgttgc
    13741 ggcccagtat ggctgtgccc ttcgcattgg ggtgaactgt ggatcaatcg atccggcaaa
    13801 acgcgagaag tttccgtccg aggacaagat ctctccgatg cttgaatcgg catgggaaca
    13861 ctgtgagctt ctcgatcggc tagggttttc acagtattgc gtgtcgctca aagaatcgga
    13921 tcctcagatg gtcatcgagg cgaaccggcg gtttgctgcg gcccgaccgg atgtgccgtt
    13981 acacctcggt gtgacggagg ccggcatgcc gccggaagga atccgcaaga cgcgtgccgc
    14041 gttgattccc cttttggcag aaggaatcgg tgacaccatt cgcgtctctt tgacgttacc
    14101 caacgaacga aagcatgagg aagtcctggc cggtttggag atcgtggccg atgtggctgc
    14161 cggacgggtc acggtggagc tttggacacg ggatgggctg aacatcgtca gttgtccgag
    14221 ttgttcccgc gtcgaaaatg ccgcctttgt gcagttgacc gaaaaggtcc gcgaattgac
    14281 ggcttatgcg gcgagctatc ccatcacgat tgccgtcatg ggctgccgcg tgaacggtcc
    14341 cggcgagacc gacgacgccg atctgggact gtggtgtggt ccgaagtatg tgaatctgaa
    14401 aaaaggcccg gtcacgctag ggagcttcac atacgatgaa atcctgccac gactaaagca
    14461 ggaactggac gccctgatcg ccgagcggtt ccccgtgaca taacccggcc agtgggatga
    14521 ccgtgtgtct ctttaccgat ttcgcctatg tgccgcggtg gtgcagcctt gcgattgttg
    14581 ttcggccttt tctgtgcaac cgcggtgccg acacggatgg ggatataacc ccaaccgagg
    14641 gtggattgcc ttacgggccc gttgaggtta cgatcatagg tggcgatcgt gatgaaggct
    14701 acgtgaccat caaggttttt ggcccatttg gccgatgaac ttggatgccg gcacaggccg
    14761 cgcacgattg cgaaaaaagc actcgaccga gcgtggtcag tgatggatcg acggtgggct
    14821 gataccgtgc ccgatgggag ccgtccccga acaagtatcc agcacgagcg tggagccatc
    14881 gtgtgagaaa cctcgggttt ctacggtgga tgacgcggtt gccggatcac ggcgcccccg
    14941 tgaaagtcgg gcctacctgt tcgccatcac tccgctcgcg agtgcttttt ttatgcgcag
    15001 gcccagtgcc gagcggtccc gacccgcgct atctttcgga gcgggcactc tcggcaacgg
    15061 acgcgatgat tgcgcttaca gcctctctga aaaagtcccc ctctccctct gggagagggt
    15121 tggggtgagg gcaagcgaac gtcggaattg attgagtaaa ccggcagcga ttcgagctac
    15181 cctcaccccc gacccctctc ccgccgaacg tgcgagggga aatcatggga taggctctta
    15241 tttctccacc ggttcgatgc ggatgacaac cgttccgctg gcgatcggtt ccttgacctt
    15301 ggcagctaaa cggaccggtt tcttgttgta tcgcacgtcc tcgtcgatct cttcttcgct
    15361 ggccacgaag gactggcctt ccatggtcag ctcaacgcgg acggcaccgc ggccttcgcc
    15421 cacaatccaa accccgttgc caacccgtcg ccacggggag taggtaatga gggcctcctc
    15481 gaaagattgc ggcgaggaca tctcaacgcg atcggtaaca atcaggcgac cacgtccctg
    15541 gcgttcgtat cggaactcgc ggaccaggct tttcagggcg ggaacattgt aggccgacgt
    15601 gatgtccatc cgataaacat cggcttcatt actgaattgc gtctcgagga ccttggccct
    15661 ggcctggctt cccgttcgtt gcagttgccc ggcaaccacg gggacgggat ggccgaaaga
    15721 gttgagcacc ttgctctcat aacgccgacc actgaaagtt cgggcggtgt aaacttctgc
    15781 acctggatca aggagaggtg tctggcccgc cagggcaaca acaaaagaac cgacatcgtt
    15841 atgattgtgg tgctcggcat tgtggccacc tttgagggcg acagccagtt tgtcaggcga
    15901 aagatcggtt gggcgacaaa tgaggatgcc ggcgtcggca aaccaatcgc ggatatcgtg
    15961 ccgccgagcg gccgcctggc ctgtgcggtt ggtcagcgag ttcggcatgg catagatcgc
    16021 tacctcaggc aggaaactac tcggcccagc accgacacca aggtaacgat tctccagctc
    16081 cttcaaaccg aggtcgaagc ggcggctaag atacgcgctt aggacgctat ctggccttga
    16141 gccaacgctg caatcggcga aggcggggta gatgttcggg gcaacctcca tgcgatagcc
    16201 aaaaagggcg atttgtggca cgccggggag ggcgaagaaa tcgagctggc cgccggtggc
    16261 ctgaaccaac gtctcgccca acagcgtgaa atgcccaaag ccgtaattcc agtaaccgat
    16321 tccttccgaa cagtatccgt ccggtgtaaa accctgaagg aaatactgga tatatttttc
    16381 agctgcggcc acgaagaaac cgcgacgctc tggagacgga atattctcca gggcggctcc
    16441 caccacaccg gccagacaca ccgcattcca attgtttgtt ccggtaatcc accacaaccg
    16501 cggattgccc gtattcacgg cctgttcgaa cggtgtaaag atacgccggt ccagttcact
    16561 ggcgatgaga cggcggcatt cgggcgacag cttttctgcc aaccaatagt cgatggtcgc
    16621 catattccag gcggtgcttg cagcccctag gtcgatttcc acggtccggc catagaaatt
    16681 gtcgagccgt ccatcgtggg cgggcattac ccaggtcttt tcctggcaga tggcggcaat
    16741 ggtcttttcc agctcggcca ggaatcgccc ccggttctcg agacattcgg ctagggccaa
    16801 attcccaagc cgccctcgcc gtgcggacat gcgggactca taccggcgcc gattgccggt
    16861 tcgggaaaag tccagatata gctcgtccgg gacctcagca atgggccggg aaagttcctc
    16921 ctcggcctcg gcaactacct gacgaaacgc tttatccttg gccaggacat cccaaatctc
    16981 tcgctgcgaa attggggcgc caacccccac aggcttcggc gggagatgct tggcccaata
    17041 ggccgccctc tcggcagaaa cacctccagc aatcggctcg gcggtggctc gatcgcccat
    17101 acccattccc agtaagacaa ccgccatgat cgccactaag ttgccagcat tttgacgaca
    17161 aaggataaaa gtttttcgtg acataatgac ctccccagac aaaggttgga acacgatgcg
    17221 ggcaaatctc aattgggcca aaacggtcag cgtcgcctct caggcgagac cgtccatggt
    17281 cattccaaaa tgcttgagca caacgtgaaa gatgtcggtg tctgtcacgg gttcccgggg
    17341 aaacacaccc ggacgcgagc taatcgccac cgttcgctgg aagcgctcac gcaccggggc
    17401 accatgcgac ccatgcacca actccggctt taggggaata ccgcgtgtgg ctggatcaaa
    17461 aaacaactcc actggatcat aacctggctt gcggtgaata tctacctttc gcgcaaatgg
    17521 cggtgccttg tggtcctcga accagtaata atatgcgaac cacgtatcgg gctgggccgt
    17581 caggatgacc tcaccacttc gcgggtgatc catatgatac ttgctccgct gctcacccgc
    17641 cagcacttca tcaattcccg gcagaccttg aaacaaccgc atcacgcggt caatgatctc
    17701 cggttcgcca tcacggacaa aaacatgggc gatctgatgg tcggccaggg cccaggccgc
    17761 actggtggtg tccaggtact cgccgtctcg ctcctcgcgg acagaaagta atcccgcttc
    17821 gcgcaagatc cggttgggat aaatcactcg ttcgacgggc gtgatggcat attctccggc
    17881 aattagccaa cagggatcac catcataagc atctcgcaaa ccgttcatca attgtccgag
    17941 gacctcgtca agctcaagta cggccttgcc ggcggcaacg ctgtccgggc cgtccttctg
    18001 ggcagcgtaa tctaaatggg gcaagtaaat ataaaacaag ttcggtcgtg ccgctttggc
    18061 caattcaatg gcagactcga cgatccaccg agtactctcg atattggcca agggacccca
    18121 ataacgatgc aaaggaaaat cccccagttt ttgccgcaac tcgccgtaga ggttctccgg
    18181 gcgcgtgtag caccacatgg tttcactgcc gtcggggttg tggatcggcg caggcgtgca
    18241 gatgaagtcg gccccagcct ccttggcatg ttgcggaaac caaatggcgc tggtcaaccc
    18301 tcgctcatga tgataaagca agtcccagag ttgtgggact tcaatgcaat cattgggcga
    18361 ggtccacatt tcgacctcgt cgcgatcacg ccagtagatt ccgttcgcaa tgatgccatg
    18421 cctcttgggc ggtcgcccgg tcgtcatgtt cgcctggacg gggcaggtca aagcgggaaa
    18481 actcggaata aggtccacaa tctcgccgcc tgccatcatt tccttgaggt tttccatccg
    18541 ggcgacatcc ttttcgcgaa ggttcgccac cgatatgagg atcaggtaat catcgagttt
    18601 ttccgccatg attcagcctc tcgtctttgt ccttgacaaa aagggagata cgcctcagga
    18661 acgtaacatg ctttgccgag atgccccaaa ccagcaccct tcagccggtg acatcactgg
    18721 gtcatcccag attgataacg ggacagaatg tcaaacgcct gtttggccgc ctggagccca
    18781 aggtgactgt gtcggctcag ttccacgtag atcccttttt tgtacccgat tttctgaagg
    18841 gaagcaaaaa cctcggcgag atcgacttcc ccctccccaa acatgagatg ttcatgcgtc
    18901 ccgcgacgca tgtcttcgag gtggatattc acaatccgcg gggcgtactg ttccacaata
    18961 gaggaaatgg gaccttcttc ctggcacagt acatggccga tatccagcgt caacaaacag
    19021 cccaaactcg gggcgcgacg gcaaagctca tcgtaatcgg ccgtggtagc gatgaacatg
    19081 ccgggttccg gttccagggc cacgggaaca cccattgcgg aggcctcgtc acatagatga
    19141 ctcaagccct ggataagcca ctcccaggcc tgctcgggag ggacttcgtc gtgtcggttg
    19201 cctgaccaaa gagaaaggca atccgcgtga agcgttacgg cacagcggag ggcgtggcga
    19261 tagaagtcaa ggcgacgcac ccgcccgctg gcatctgggg agatcaagct tggttcatgt
    19321 ttgtgccagg gatcgaggag aaaacgggcc cctgtctcga tgacgcagta catccccagg
    19381 cgatcgagca cgtcccgtag ttcgtggagt tgccgctccc aatagagatt gaagggcgaa
    19441 aggagatagt ggtcaatcgt cagggccacc ccacggtaac cgatcttcgc caagccgcgg
    19501 agggctgccg tgggttcgtg gtggaccaga ccgttagtgt tatagcccag gatcataagc
    19561 ctctcgcttt tcctcatgtg ttacccattt gacccacggt agcacctggt ggcaaggccc
    19621 ggtgcctccg gacgaaccgc tgatttttgt ctactaagga tgtcctttga ggcagagttc
    19681 gaacgggcgg tccccgcgtc accacttgcc caaatatgct aaaatgggtg gttccttatg
    19741 aacactagga aggaatgcga tcatgcggcg ctggaaaact gtatctattg tcggcgtggg
    19801 cctgattggg gcctcgatcg ggcaagccgt cctgcggcgc ggtctggcgg atgaagtcat
    19861 cgggatcgca cgaaggcaaa caacgctccg cgctgcgcgg cgtgtgggcg ccgtcagcca
    19921 tacgaccatc gatctggacc gcggcgtcgc ccaagctgac ctgattatcg tctgcacgcc
    19981 ggtggggaca attcccgatc tggttcggcg ggtggccgag gcctgtcccg aacgtgccct
    20041 catcagcgac gtgggcagtg ccaagggcca gatcgtggct gccttggatg aggggttacc
    20101 gcggggctgc cggtttctcg gggcacatcc cttagccggg agcgaaaaga ctgggccaag
    20161 ctactccgat cccaatcttt ttgaaggccg ggtgacaatc atcacaccga ccaagaacac
    20221 gcgggcagaa gacttcgatg ccctagagga tttttggacc ggcttgggat cggtggtcat
    20281 tcgcatgtcg cccgaagaac atgacaaggc cttggccatg accagccatt ttccccatct
    20341 tgtggcgtcg cttttggcgg ggatgctgcc cgaaccttgg ttccgcctca ccggcagcgg
    20401 attcttcgac acaacccgca ttgcatcggg tgatgttgag atgtggaagc aaatctgcat
    20461 gcagaaccgg gagaatcttg tcggcgcgtg ccaggccttt gccgacgaac tgggacggct
    20521 ggcccgagcc ttggccgccg gtgatgaaag cacactggaa cagattctac tacgaggaaa
    20581 aaagaaccgc gatgctctgg gaagttgatc tttttcctgc cgagggacaa cccgatgtgg
    20641 cggcccgcca gattcggcag gatgcccacg acctgggaat ccacaaagac tggacgatcc
    20701 tcacgtgtca tggctacctc atcgagggcc agttgacgga ggctcaagtc cggcaagtga
    20761 ccgaggagtt actcgccgac cccgtcgtgg aacgtagttt ggtagccccg gtgggcgatt
    20821 ggtcgttaac ggcgccaccg caaccgggca tggaggtcat ttacgtcctc ccaaagccgg
    20881 gggtcatgga ccctgttgcc caaagtgccc aatcggccat cgccgatttt ggatggaagg
    20941 ccgaccaggt ccggaccttt cgcaaatact gggtagctgg cctgtcgcag gcagaaatca
    21001 aacggctctg tcaaaaaatc ttggccaacg acgccatcga gcaggtcgtc ctgggaccgt
    21061 tgccttttcg caccttggaa caaggtcggc cctacgagtt ccgtcttatc acggtgccca
    21121 ttcgcgacat ggatgacgag gccttggtgc ggctcagtcg cgaggggcag ttgtttctct
    21181 ccttggtgga gatgcagaca atccgcgagt actttcgtca acaaggtcgt gaccccaccg
    21241 atgtggaatt ggagacgctt gcccaaacct ggagcgagca ttgcagccac aagaccctgg
    21301 ccggccggat tcgctatcaa gacgatcagc ggcagatcga attcaacaac atgctcaaag
    21361 agacgatctt tgccgccacg catgaaatcc gcaaaagtct tggccaggac gactggtgtg
    21421 tgagtgtctt tgaggacaac gcgggcgtcg ttcgctttga tgagcggtac catgtgtgct
    21481 tcaaggtcga aacgcacaat cacccgtcgg ccctggaacc ctacggtggg gcgaacaccg
    21541 ggatcggcgg ggtgattcgc gatccgatgg ggacgggatt gggtgcgaaa ccaatttgca
    21601 gcacagatgt cttttgcttc gcaccgcccg acgtctccca cgatgagctt ccgcccggcg
    21661 ttcttcatcc ccagcgggtc atgaaaggcg tcgtggcggg ggtccgcgac tacggtaatc
    21721 ggatgggcat tcccacggtc aacggcgccg tgtatttcga caagcgctac ctagggaatc
    21781 ctcttgtgta ttgcggcaat gtggggttgc tgccctgtga gaaggcacga aagatgccac
    21841 aacccggcga cctaatcgtg gctgtgggag gccggacagg ccgggacggc attcacgggg
    21901 ctacctttag ttccgccgaa ttgacaaccg agagtgagac cctctccggc ggggcggtgc
    21961 agatcggcaa cgccatcacg gaaaagatgc tgctcgacgt cctcctggaa gcccgtgatc
    22021 ggggcctata caacgcggtg accgattgtg gtgccggcgg tttttcttcg gcggtcgggg
    22081 agatgggtcg cgacatcggg gccgaggtgg aactctccca tgttcccctc aagtacgaag
    22141 gcctctccta taccgaaatc tggatttccg aagcccaaga acgtatggta ttggccgtcc
    22201 cggaaaaaca ctggcccgaa ttgaaggccc tcttcgaatc ggaaggcgtg gaagcgaccg
    22261 ttatcggccg attcgtgcca acaggtcgcc tcgtccttag gtacgcgggt catgaagtgg
    22321 gcaacctgtc aatggagttc ctccatcacg gtcggccccg agtggtccgc gaggcctttt
    22381 atcgtccgcc ccaggttcgg ccgctgatca tcaaagacaa cgcggaggcc acggcgggcg
    22441 aggacctttt ggctatcctt cgctcgccca atgtgtgcag caaacactgg atcatccgcc
    22501 agtatgatca cgaggtccaa gggggaagcg tcatcaagcc cttggtggga cgatgggacg
    22561 acggtccttc cgatgccgcg gttttgcgac cggtgctgga ttcttaccgc ggccttgtca
    22621 ttgcctgcgg catgaatccc cgctacggcg atctggatcc ctactggatg gcagcctccg
    22681 ccattgacga ggctattcgc aactgcgtcg ccgtgggggc tgatccccgg cggatcgcca
    22741 tcctcgacaa cttttgttgg gggaacaccg atcgacctga gacgctgggg tccctggttc
    22801 gagcggccct cggctgtcgg gatatggccg tggtcttgca aacgccattc atcagcggca
    22861 aggatagcct caacaatgag ttccgcgtca gcgagacgga aaccatcagt atcccaccgt
    22921 cgctcctcat cagcgccatg ggacagatcg acgatgtccg gcgatgcgtg acgatggacc
    22981 tcaagcagcc gcacaacgtc atctatcttg tgggaatgac ggccgatgaa atgggtggct
    23041 cccattggtg tctggtacgg catcaggaag gcggacgtgt cccccattgc gatccccatc
    23101 gggcaaagcg gatcttttcg gccctccacg cggccattga agcgggttgc gttcgggcgt
    23161 gtcacgacct cagcgaaggc ggattggccg tcgccgctgc cgagatggcc ttcgccggct
    23221 gctggggcat ggaacttgat ctggatcacg tgccgtgcga aacgtcatcc ctcgccccaa
    23281 tcattcgcct ctttagcgag tcgaacaccc gcttcctctg cgaggtccca cccgagcggc
    23341 agagccactt cgagagctac ttcgcgggtc ttcccgtggc caagattggg atggtcacag
    23401 aagatgaccg ctttgtggtg cggttcgccg ggcggacgct aatcgatctt cccttgagga
    23461 cactcaagga ggcatggctg agtccactgg cttggtaagc ggagcggtga cccacggcag
    23521 aaaaaatggc ggaaagactg gcaacagcga tgtgaaagaa aggccccatg gcaaaaccac
    23581 gagtcctcat tttacgtgcc ccaggtacca actgcgacat agaaacggcc tacgcctttg
    23641 aaaaggccgg ggccgtgacg gaccgcattc acattcggcg gctactcgaa caaccggcca
    23701 ccattcgaag ctatcagatt ctgtgtgtgc caggcggttt tagttatggc gacgacgtgg
    23761 ccgcgggcaa gattctggcc gtccagttac ggcatcacct tttgcatgtc ctggaagagt
    23821 ttcaccaggc cgataagcta attctaggca tttgcaatgg ctttcaggtc ttgctaaaaa
    23881 ccggcctgat cgttccccct gacgaaagcg gtaagccctg ggcgacgctg acctggaacg
    23941 agtccgcccg atacgaggac cgctgggtcc atcttgaggt ggccagcgat cgctgcgtgt
    24001 tcctgcgcgg catcgaacgc ttgcaactac ccattgccca tgccgaagga cgcttcgtgg
    24061 tccggcaggc gagtgggctc gatcgtctgg cccaggcagg ccaaattgcc ctgaagtatg
    24121 ccctgcctat ggaacttaag cgaatggccc ggcaggagaa tgcggcgtct cgacaatctg
    24181 ttgaagcggc gcagatggcc acacgctgtt tgggaggagc gagtctctct gccttggagt
    24241 ccgccgtggc caaggcgaat gatcagcgat tgccttatcc ctggaatccc aacggatcaa
    24301 tgggaaatat cgcgggcgtg tgcgacagga cgggccgcgt cctaggactg atgccccatc
    24361 ccgagcggtt cattgatccc acccaccatc cccgctggac ccgtggcgaa tggcac
//
LOCUS       DAOSUT010000055        24269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1356_length_24269_cov_9.702527, whole genome shotgun sequence.
ACCESSION   DAOSUT010000055 DAOSUT010000000
VERSION     DAOSUT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_24269_cov_9.702527"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLD05_06850"
     CDS             <1..924
                     /locus_tag="PLD05_06850"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77190.1"
                     /translation="TQMGNQGMATDSFRRTLEQLEDGVIGHIREAHVWFVFGGSGPRQ
                     PPQDTPPVPSGLDWDAWLGPAAERPYHPQYVSGWGGWWDFGTGCLGGGGSHSLHLTFK
                     ALGLRQLWNPPHNSEKRIRVESEIPEKAPHGFPRWQFVRFYIPARGDQPPATIYWYNA
                     DEAELRRRGVWKKLEDIAGRDLEWKDNSWTPRSGTLIVGEKGVVHTNAHNSVCQILPA
                     DKFPQQAGRPKRYPHVAGHEYEWLNACRGQGSCLSQFDHSGPVIELLLVANVAGRFDE
                     PIEFDPVECRIIGNAEADALLRPPYRTGWSL"
     gene            1182..3209
                     /locus_tag="PLD05_06855"
     CDS             1182..3209
                     /locus_tag="PLD05_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP77191.1"
                     /translation="MNMRMWPWVMVLTAGCLVLGFHRCLAAESTGAVSPNEQAIQEVE
                     AGQRDTANAAWWGFYPEDSTQCLQAALRCKAKRVIIPKMASPWVVDKLELIGNKEIIF
                     EPGVEVVAKRGAFRGKGDCLLTAVNQENLTLVGNGATLRMWRDDYAQPPYEKAEWRHC
                     LSLRGCRNVIIDGLKLCESGGDGIYLGAGKNGEPNEKIVIRNVVCDRNYRQGISVITA
                     RDLLIENVVLSNTAGTPPEAGIDFEPNRATEVLINCVMRNCLIENNRGYAIHLYAGAM
                     NTESQPISIRLENCRTKGTNAGSLSIVTRNRAGEAVRGFFEVSTCHFEDSENAGVIIR
                     SKPADGFQVRLQNCEIVQLTENPKWPAPVTFVSRPGDENDVGGVVLDNLVVRENTDRP
                     LIHFNKGGGVCLKNVVGTATIYRGADRKTVSVDEDLILQWYPCSPIRQLPIVATDRLA
                     VWPTPSTQESLREVTRHRLRQIGNYVFYAKKGDRVTFEMAYDQIGKNAGGRLPVRVFG
                     PAGNTVHTSELRFQEQTRLEFTIPEMGVYRVRAEPGPNAVRLVRTSHPVAILGERGII
                     HLIGTTGRFYLLVPPGKSLGLEVSGDPPGECVAACLFEGTSQPLWKITGCEDAKACLV
                     DPATVERVLCLELARPSQGVLEDEYIVIRGVPAVLSFWPDVLFVDSGEGRE"
     gene            3236..4975
                     /locus_tag="PLD05_06860"
     CDS             3236..4975
                     /locus_tag="PLD05_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP77192.1"
                     /translation="MNPQRQVSVGVNSGARLQLDLLKTKASMVVAVVLGATLWWFLSG
                     SAKAAQPVGNQPTYSSPSPLAVNDGGGNGANQSVIASIHHVATATRDASKSRRPNIIL
                     ILADDLGYGELGCYGQQKIKTPNLDRMAAEGMRFTQFYAGAPVCAPSRCVLMTGKHLG
                     HAAIRDNSEVQPEGQWPLPAEEITIAEILKGCGYATAAIGKWGLGPPGSSGDPNAQGF
                     DLFFGYNCQRHAHNHYPTWLYRNREKVALRNPEFPAHQRFPEDADPNDPSAYARYIGE
                     DYAPDHMLAEALAFIRENRDRPFFLYYATTVPHLALQVPRDSIQEYLGQFPETPYLGD
                     RGYLPCFAPRATYAAMVSRLDRDVGKILEEIKQLGLDSDTLVIFTSDNGPTHGRGGGL
                     EDGVGGSDSLFFQSAGPLNGLKGSVFEGGLRVPMIARWLGKIPPGTVSDHVAVFYDFL
                     PTFAEIVSADVPAGIDGVSFLPTLLGRPEQQKTHDFLVWEFYGYGGQQAVRFGPWKAI
                     RRDCYKSLDGPLMLFNLENDPGETRDVSSEHPELVQKAELIIKTEHRDSPFWDFPTKR
                     RGPPKTLDLQTTQ"
     gene            5084..5905
                     /locus_tag="PLD05_06865"
     CDS             5084..5905
                     /locus_tag="PLD05_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77193.1"
                     /translation="MIRAVLRKAVRDGLPQLVVSCVLLFLFSWLFVWLTSLINVGLWA
                     TFFDMLPAFVQKLMPVSPKILITPTGRVSFLFVHIVPLLIIIGWSIGRASQIVSREIA
                     SGRYEVLLTLPVHRYLWVVVPAVVISLGAILMAGSMRLGLWVATMTVRLPERLPLAQF
                     TPGLVNVAALSIALSGITAAVSAFLSNRWNTIGIAAVILMVSMIVKLVARLWAPGDWM
                     KYLSFLTLFEPQELIFAGGATGVVIGYNAGLIAIGVVGFVVAAAALSLRDIPVSR"
     gene            6253..7239
                     /gene="floA"
                     /locus_tag="PLD05_06870"
     CDS             6253..7239
                     /gene="floA"
                     /locus_tag="PLD05_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650218.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOP77194.1"
                     /translation="MSDITSGLVAQVPWWSIWLIVGIIGGVILITFVIVVLAYGALWF
                     QAYMSSARVSMWSLIGMSFRQVNARLIVQAKIMAVQAGLGNERETGISTRRLEAHYLA
                     GGNVMNVIKALIAAHRAQMHLTFDQAAAIDLAGRDVLDAVRTSIYPKVIDCPDPRSGR
                     TMLSAIAKNGVELKVRARVTVRTKMETLIGGATEETVIARVGEGIITAIGSAETHLEV
                     LEHPDRISKAVLERGLDQQTAFQIVSIDIADIVVGQNIGARLQYDQAEADTRVARAKA
                     EERRALAMARSQEMKAQVAYNRAQVILAEAQVPIAMAKAIREGKFELIPQRN"
     gene            complement(7348..8355)
                     /locus_tag="PLD05_06875"
     CDS             complement(7348..8355)
                     /locus_tag="PLD05_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOP77195.1"
                     /translation="MSVDLRTSYLGLKLKNPIVVGASPLTESLDNQKRLEDAGAAAIV
                     LPSLFEEQILAEEENLAKVHEFGTDSFAEALSYFPEPEQYRLGTDEYLDRIREAKKSL
                     SIPVIASLNGTTEGAWIRYAKLFEEAGADAVELNIYSVAADLNQSASNVESQYCHLVK
                     AVASSVSIPVAVKIGPYFSSVGHFVQQVVNSGAKGVVLFNRFLQPDIHLETLETKPHL
                     VLSTEFELLLPLRWIAILYGRINASLALTSGVHSANAVLKSILAGADVVMLVSALYKQ
                     GFGSITNFLNQIQEWMAEKGYESIEQMKGSMSHKNNPNPTVFERGNYMKALTSFVGTP
                     I"
     gene            complement(8392..11973)
                     /gene="nifJ"
                     /locus_tag="PLD05_06880"
     CDS             complement(8392..11973)
                     /gene="nifJ"
                     /locus_tag="PLD05_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOP77196.1"
                     /translation="MTQQRFVTVEGNEAAANIAHLCSEVMAIYPITPSSSLGELPDAW
                     AAAGRKNIFGAVPLVIEMQSEAGAAGAVHGALQGGALSSTFTASQGLLLMIPNLFKIA
                     GELTPSVFHVTARTVATHALSIFGDHSDVMACRSTGWALLASASVQEAQDLALIAYAA
                     TLESRVPFIHFYDGFRTSHEVAKIEQLSDDDVRAMIDMELVRAHRERAMSPEKPVLRG
                     TAQNPDVFFQAREACNPFYDKTPAIVQKAMDKFAKLFGRQYHLFDYVGAPDADRVVVI
                     MASGTGVVEEAVDYLVERGEKVGMVKVRLYRPFDADALVRVLPKTVKRIAVMDRTKEP
                     GALGEPLFLDVVAAINERWRDHALGTELPLIIGGRYGLSSKEFNPAMALAVFEELKKP
                     QPKRRITVGINDDVTHLSLSYDPTFSIERPDVVRALFYGLGSDGTVSANRSSVKIIGE
                     NTPFYAQGYFVYDSRKAGSMTISHVRFSPRPIKGSYLINKANFIACHQFHFLERIDML
                     SAAEEGATFLLNSPFPAEEVWDHLPAEVQKQIIDKKLKFYVVDAYRVAKETGLGVRIN
                     TVMQTCFFALANVIPVEQAIKEIKDQIVKSYGKKGGSAIIEKNFAAVDKALESLREVK
                     VPDRVTSNIHMVPPVPEDAPPFVKNVLGPMIAYRGDDLPVSALPVDGTFPTGTTKYEK
                     RSIALEIPIWDPKICIQCGLCSLVCPHAAIRMKAYDPAVLANAPEGFKSAPWRGKEYP
                     GYHVTVQVAPDDCTGCGLCVEVCPVRDKEVAKHKAIDMCFKLDHLDRERACFDFFLKI
                     PDLDRSKVAVDTVKGSQLLLPLFEFSGACAGCGETPYVKLITQFFGDRMMIANATGCS
                     SIYGGNLPCTPYTTNAEGKGPTWNNSLFEDAAEFGFGMRLAVDQQIEYATGLLKKLAP
                     QIGDTLVTELLNNVQETDEQIAKQRQLVAELKKKLATIPGDDAANLLASADYLIRRSI
                     WSFGGDGWAYDIGFGGLDHVLASGRDINLLVLDTEVYSNTGGQASKATFRGAVAKFAA
                     AGKQGRKKDLGLMAMSYGNVFVGQISLGANPLHAIKVIRAAESYRGPSLIIAFSHCIG
                     WGIDMVKGMEYQKEAVACGYWPLYHYDPRDKEQPLHVDSRPPKGAFKDFALKQARFAI
                     LSRSKPERAETLFQLGQEDINFRYALYDNLSSALARAGAKPEGGDGQ"
     gene            12682..13257
                     /locus_tag="PLD05_06885"
     CDS             12682..13257
                     /locus_tag="PLD05_06885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOP77197.1"
                     /translation="MSLLKTLAGIVVVAVNLVSQKLAPREQTPVELRVGVVAPDFELP
                     GSDGKTYRLADFRGQKIVVLAWFPKAFTPGCTRECRVFAERADEFANLPVAFFTASVD
                     TPSENKKFAESVGARYPILSDPDRKVALAYGVVSSPRDFARRWTFIIGLDGKILHIDK
                     NVKVDTHASDVARWIKEYLKANPPAGDSGKR"
     gene            complement(13445..15094)
                     /locus_tag="PLD05_06890"
     CDS             complement(13445..15094)
                     /locus_tag="PLD05_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77198.1"
                     /translation="MTRVKRPLWLTKSLGLIAGLAVVFSALPVLAGAPAVVVRLAPLA
                     QWQQNAAFLADMLKEHPSSVRIQMLVGLLDAVGKNGVDPTRPMGLACLVGEQGNPILV
                     GCIPTSNLDQLHTFAKDWISEPTLISDNVYHVKVRDRELYVRLYDAWVVVSHNAEILS
                     SLSGDPAMWLAEFDSSMDVSIAINPRELASLRGVFRNWLERQEALAAMLSRIEVSQEV
                     RQSVLRPLLETAKEAVQQTEKVILSYRIDRNEKKLVGNCTWTAKAGSDLAAQFAQERL
                     VTSPLVAIRLWNDTAMTMAWSVTFPPPSEEDLERIGSLIQSRWDKQIERRISNSVDAQ
                     VAKNLAHDFLGLLGDWAREGKADGIVTLVTKPDQVTLLIGGYVGNSTGLEAWVGNVVE
                     SAQQRAPKAFERVTIIPDHTLHGDASIHLMRIALGDRLSPEQKAVLGDDLEVAFTASN
                     GYAAVAIGKNPVDVIKRAIDDAVSQGDQTAPAFELCLKVDDLVATAAKLNPQDSRLQT
                     LKANLEQCEGVQLLRKKVDFTSQQMVVDFEMDLNWLRLLKR"
     gene            complement(15188..15772)
                     /locus_tag="PLD05_06895"
     CDS             complement(15188..15772)
                     /locus_tag="PLD05_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77199.1"
                     /translation="MTLHHGIDEQNLRREPLAELLKREAEAERPAFSEDLYGRILNQV
                     GIPGAERAHLFSSGKSRLSFKQIAWVVLATYASVVTVAILMQVVQRSGWFHRQHIEVS
                     GIAGENLNEPRQEKGSGSDVNLVHGKPPTEAIEDLVSAPDRTIQQVQIALSEIDRQRW
                     ANLDNDLRLAGELLHQQLPWGVLSRAESSQVETP"
     gene            complement(15896..16504)
                     /locus_tag="PLD05_06900"
     CDS             complement(15896..16504)
                     /locus_tag="PLD05_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP77200.1"
                     /translation="MSHLASLTSLTDEQLAEQAQMGCLASFAELVRRYQVPVLHFLQR
                     KCRSSTDAEDLTQETFLRIFRKLSHYRVGAPFRPWVFTIAYRVALNGMRGHRYSTVGE
                     NEKSSRITQVDSLLEDAEENHRLWDAVQAELSPIQFTAVWLFYVEELSIRQIAAVIGK
                     SESAVKTILCRARKVLVHRLRAYAKFNTLPVSEDEAVSPERH"
     gene            complement(16662..>17517)
                     /locus_tag="PLD05_06905"
     CDS             complement(16662..>17517)
                     /locus_tag="PLD05_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77201.1"
                     /translation="QVPDNWRWIVHFDIDQLRDSKVAATLFSEFWHAHPERHKIEAIA
                     ALAGLNLKKDLHGVTFLGTRFDPKFGAMIIHCRMNPDLLVGLIASEPTYRRRESKGLI
                     LHQWTDRNDNSEITGCFLDQDHLVLGKDATAVEVIIERYQQKSSQERPDFFNVRKGTY
                     LQIHANELQELSVPFMSPLLRRSRRLSAMVGEQSGQFFLQCSFEVEGAEIALDLKDAL
                     TGLVAIGKLHYADRPEYVAGLTLIRVFASENKTTMVWTMPAEPALRVLKEIASQVATE
                     GKKSGGLP"
     assembly_gap    17518..17617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17618..19266
                     /locus_tag="PLD05_06910"
     CDS             <17618..19266
                     /locus_tag="PLD05_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77202.1"
                     /translation="NGGVVNRFVFWSCLDQRKGASMPNRREFLGMGCGLAMSGLLGSA
                     GTLLCPKHGSASTGSEGEETIRTDFLDGSSKSVAWLEEMVGPSLGRLLTKTSREIRSS
                     PFSVGLETLDRSMYDPERVYDPLGKLGVKWARLQTGWARTEQIVGQYDFGWLDAVVDA
                     VVGQGVAPWFNVGYGNKLYTPEAPDASAVGWIPIYNADAQQAWLRYVRALAQRFGERV
                     KHWEIWNEPNIKNFWKPGEPSPELYVEFVAQTAKVIREVIPQVTIIGGALAGMPTDYL
                     KKLLSCGLAQYVDKISFHPYRAIPEDGYRDTLETWKQLLQSAGEKRLQLWQGENGCPS
                     RPGSAGALAQFPWNEITQCKWLLRRVLYDRVLGLELTSYFHMVDLMRYNWGQGPTDKA
                     NWKGLLRGEDYSPKPAYFGYQRICTLFDDDARRDISLDTSVEVVEENRADPASSRSSK
                     EKRFIPPAAFVRNNSPMWCFWYASDLIKPGEITYLTLTIREKALSSGELFYLDLLSGW
                     IFRLSKLERQNDQCHVRSIPVGDYPVVIGAPGALQGLVSS"
     gene            complement(19520..>19722)
                     /locus_tag="PLD05_06915"
     CDS             complement(19520..>19722)
                     /locus_tag="PLD05_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77203.1"
                     /translation="AKKAAKKAAKKPAKKAAKKPAKKAVKKAAKKVAKKPAKKPAKKP
                     AAKKAPAAPPAPAETTPPPAGG"
     assembly_gap    19723..19822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19823..20092
                     /locus_tag="PLD05_06920"
     CDS             <19823..20092
                     /locus_tag="PLD05_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77204.1"
                     /translation="AFFAGFFAAFLAAFFAAFFTTCLATFKTSLLKDTLGAVVEDICH
                     VTDPQRAPYHNTNYHVPVSAPKRIAPKSTSVFACLAAAAFFTAAA"
     gene            complement(20216..21382)
                     /locus_tag="PLD05_06925"
     CDS             complement(20216..21382)
                     /locus_tag="PLD05_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77205.1"
                     /translation="MAKELRIGMLGCGFMGRAHSNAWLQVSHFFDREYKPVLKACYGR
                     EEDRQQLETFAKRWGYESIETDWRRLIERPDIDLVDVCVPNHMHYDCVIAAAQAGKII
                     VCEKPLAMNAQQAEEMVAAVEKAGVPNMVSFNYRRVPAIALAKQIIEEGRIGRPFHYR
                     ATYNQDYTISTKVPQGGLALWRLDARIAGSGVTGDLLAHSIDTAEWLNGPIKKVVAYT
                     ETFVKERVHAETGKVEKVNIDDACMFLAVFENGSLGTFESTRYARGRKNYNTFELNGE
                     LGSVFFDLEDPHILQFFRYADPETGKKIEDHLTGWQRIHVTHSEHPYMKHWWVPGCTI
                     GYEHTFTNSFADFLASLEGGPKFQPDMRSALRTQRVCDAVLQSAREGRWIEIPA"
     gene            complement(21452..22522)
                     /locus_tag="PLD05_06930"
     CDS             complement(21452..22522)
                     /locus_tag="PLD05_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP77206.1"
                     /translation="MATRNNFPKLHNAAWPGVVGKGDGGEPPIDLDTMLDLTAAAEVD
                     GIKFEGFDLFLYLPHIDIDSTVKSDDAIKQLADKATKRNLKIGTVVAPIWPPTGGGSA
                     MGDESERRKFLDQVRKGCRVAMVLRELGVRPYGCVRIDSACSVSDWLSDPEANQRRIA
                     ETFRQACDIAADHGEKLAAEGEICWGGMHSWRRMIQLLEMVDRPNVLGFQADMAHTLL
                     YLLGYNAPEDAILPQDFDWKDSERFYAAYKQLTDALRPWTIDLHIAQNDATVHGSGSH
                     DKTGRHCLATDPNGKLDIPRAAGFWLRDENGNVTKRMRHICWDGCMFPNAVMMNPQTW
                     NDILRVMIQVRDQHGWYEEEAA"
     gene            22833..>24269
                     /locus_tag="PLD05_06935"
     CDS             22833..>24269
                     /locus_tag="PLD05_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP77207.1"
                     /translation="MKRRSGFTLVEILVATAISLIMLGAVVAVFASVMQSINQARAVL
                     ETSDRLRSAAELLRADLKGLTVIPVPPRDPANNEGYFEYTEGPIGQNGQIRPFAGAHP
                     LAVVNLDDLDAAGNPYQDSTVGDNDDILMFTTRSTGRPFVGRCLVSGQPTTIESDVAE
                     VAWFVRGNRLYRRLRIVAPKAMALCDVNANGRLDPAEWIPANDTGAVNFNRYYDVAVH
                     FDRNAGGWVFSTLGDLTKPENRYAHLIGDPLNPASFPFHPHRAGWQPLGLPTLAESAS
                     AGAATTWLTSGLLPALSLTSLAAPLDKWDLWTNPYPVAEVDPQTGLLRTVVDPQTGLL
                     RTLEQLYRESEDLVLTNVVAFDVKIWDPTAPIGAVQTGGVVRPGDIGFFDLAFHQTDD
                     RGVFDQKFPYVGAYVDLAYAPTIEKLSTFSGFPLAKTRLASAGLAVYDTWSSHYEKDG
                     IDQDGVGGVDTGTDGFDNDNDGLVDEFDE"
BASE COUNT         5287 a         6467 c         6653 g         5662 t
ORIGIN      
        1 acgcagatgg gcaatcaggg aatggcgacg gactcattcc gcaggacctt ggaacaactt
       61 gaggatggtg tgattggcca cattcgagag gcgcatgttt ggtttgtttt cggtggtagc
      121 ggcccgaggc aacctcccca ggacacgccg cccgttccct cgggcttgga ctgggatgcc
      181 tggcttggac cggctgccga aaggccatac catccccagt acgtttcagg ctggggaggg
      241 tggtgggact ttggcacggg ctgtttgggc ggtgggggtt cgcattcgct tcatctgacc
      301 ttcaaagcct tgggcctacg tcaactttgg aatccgcccc ataattccga aaagcgaatt
      361 cgcgtggaaa gcgaaattcc ggaaaaggca ccacacggtt ttcctaggtg gcaatttgtc
      421 cggttttaca tcccggcaag gggtgaccaa cccccggcga caatctattg gtacaacgcc
      481 gacgaagctg agttacgtcg gcgaggagtc tggaaaaaat tggaggacat tgcgggccgc
      541 gatctcgaat ggaaggacaa tagctggacg ccacgttcgg ggaccttgat cgtgggagaa
      601 aagggcgtcg tgcataccaa tgctcataat tccgtctgtc aaatcctgcc ggcggacaaa
      661 ttcccccagc aggcgggcag accgaagcgt tatccccatg tggcgggcca cgaatacgag
      721 tggcttaatg cctgccgagg acaagggagc tgcttgtccc agttcgatca ctcggggcca
      781 gtcatcgaat tgttgctggt ggcgaacgtg gccggtcgct ttgacgaacc gattgagttt
      841 gaccccgtgg aatgtcgcat tatcggcaat gccgaggcgg acgccttact ccgtcccccg
      901 tatcgaactg gctggtcgct gtaggtgatt ttggataaag ttttgccgaa tccaacgcgc
      961 tgcttcggcc gacggtggtg ccggctttca tttggcacgt tgcccagata acgacgttgt
     1021 ggccgagcat tttggagaaa gttagaatac gtaaggcctc agcgtctgac cgaggggccc
     1081 gtgaagcgag tgcagctttg cacaggaaac acgggctttt gtaccctttg tggtcgaaac
     1141 ctacgtcgtt gatcgggcgt tttctgccgg aggagatcga catgaatatg aggatgtggc
     1201 cttgggtgat ggttctcacc gccggttgcc ttgtactggg ttttcatcgt tgtttggctg
     1261 cggagtcaac aggggcggtg agccccaatg aacaggcaat acaagaagtt gaggcgggcc
     1321 agcgcgatac ggctaatgca gcctggtggg gcttctatcc cgaagattct acccaatgtc
     1381 ttcaggcagc cctgcgatgc aaggcaaaaa gagtgattat tcccaagatg gccagccctt
     1441 gggtggtcga taaactcgag cttatcggta acaaggagat catctttgag ccgggtgttg
     1501 aggtcgttgc caaacgaggg gccttccgcg gcaaaggtga ttgcttgttg acagcagtca
     1561 atcaagaaaa cctcacgctg gtaggaaatg gggccacgct gcgaatgtgg cgggatgatt
     1621 acgcccaacc gccctacgaa aaagcggagt ggcggcactg cttgagcctc aggggatgtc
     1681 ggaatgtgat tatcgacggt ctcaaattgt gtgagagcgg cggtgacggc atctatctcg
     1741 gcgcggggaa gaatggcgaa cccaacgaga agatcgttat ccgaaatgtc gtttgcgata
     1801 ggaactatcg ccaagggatt agcgtcatta cggcccggga tttgctcatc gaaaacgtcg
     1861 ttctcagcaa taccgccggg acaccaccgg aggcaggtat tgatttcgaa ccaaatcgcg
     1921 ccacggaagt cctcatcaac tgtgtgatga gaaactgctt gattgaaaac aaccgggggt
     1981 atgccattca tttgtatgca ggcgcaatga ataccgaatc gcagcctatt tcgatccggc
     2041 tggaaaattg ccgcacaaag ggcacgaacg ctgggtcgtt gagcattgtc actcgcaata
     2101 gggcgggcga agcagttcgt ggttttttcg aagtcagcac ttgtcatttt gaagattccg
     2161 agaatgcggg cgtcataatt cgctctaaac cggccgacgg ctttcaggtc cgcctccaga
     2221 attgtgaaat cgtgcagtta acagaaaacc caaaatggcc ggcgccggtc acttttgttt
     2281 ctcgcccggg ggatgagaac gatgtcgggg gagttgtgct agacaacctc gtggttcgtg
     2341 agaacactga ccgtccgctg atccatttca acaaaggtgg tggagtctgc ctaaagaatg
     2401 tcgttggaac ggccaccatt taccgcggag cagacagaaa gaccgtgagc gtcgacgagg
     2461 atcttatctt gcaatggtat ccctgtagtc cgattcgtca gttgcccatc gtggcgacgg
     2521 accgactcgc ggtctggcca acgccctcca cgcaagagtc gcttcgcgag gtaaccaggc
     2581 ataggcttcg gcagatagga aattacgtgt tttatgcaaa aaagggggac cgcgtgacgt
     2641 ttgaaatggc ctatgaccaa attggcaaga atgctggggg acggcttccg gtgcgggttt
     2701 ttggcccggc cggtaataca gtgcatacgt cagagctccg ctttcaggag cagacaaggc
     2761 tggagtttac cataccggaa atgggtgttt atcgggtgcg ggcggaacca gggcctaatg
     2821 ccgtccgatt ggtgcgaacc agccatcctg tggccatcct tggagaaagg gggattatcc
     2881 atttgatcgg caccacaggc cggttttatc tccttgtgcc gccgggcaag tcattagggc
     2941 ttgaagtgag cggagatccg ccgggggaat gtgtggccgc ctgcctgttc gaagggacct
     3001 cgcaaccctt gtggaaaatc accggatgcg aggatgccaa agcatgcctg gtggacccag
     3061 cgactgtgga gcgtgttctc tgtttggaac ttgcccgacc gtcgcagggt gtgcttgaag
     3121 atgaatacat cgtgattcgg ggtgtcccgg ccgtcttaag tttctggccc gacgtgcttt
     3181 ttgtcgattc gggtgaagga cgtgagtaac gaaaaaagag ggaagggagc ctatcatgaa
     3241 cccgcaaagg caagtcagcg tgggagtaaa ctcaggtgcc cggctacaat tggacctcct
     3301 taagacaaag gcgtcgatgg tcgtagcagt tgtcctcgga gctactctgt ggtggttcct
     3361 gtcaggttca gcaaaggctg cccagcccgt aggaaatcag cctacgtatt caagtccctc
     3421 accgttggcg gtgaatgatg gtggcggaaa cggcgcgaac cagtcggtga tcgccagtat
     3481 tcatcacgta gctacggcaa cgcgcgacgc atcgaagtca cgccgtccca atatcatcct
     3541 cattttggcg gatgacctcg gatacggtga gttaggttgt tacggacagc agaagatcaa
     3601 aacccctaat ctcgatcgta tggctgcgga gggaatgcgg tttacccagt tttacgccgg
     3661 tgcaccggtt tgtgcgcctt cgcgttgtgt gctcatgaca ggcaaacatc ttggacatgc
     3721 tgcgattcgt gataattcgg aagtccagcc cgaggggcaa tggccgttgc ccgccgagga
     3781 gatcaccatt gcggaaatcc taaaaggttg tggctatgcg acggctgcca ttggcaagtg
     3841 gggacttgga cctcccggct caagcggtga tcccaacgca cagggatttg accttttctt
     3901 tggctacaat tgccagcgtc acgcacacaa tcattatccg acgtggcttt atcgaaatcg
     3961 ggagaaggtt gcccttcgca atccggaatt tcccgcgcac cagcgttttc cggaagatgc
     4021 tgatccgaac gatcccagcg cgtacgcacg atacatcggt gaggattatg ccccagacca
     4081 catgttggca gaagccctcg ccttcattcg ggaaaaccgt gatcgcccct tctttcttta
     4141 ttacgcgacc acggtgccgc acttggcgtt acaggtgcct cgagattcga tccaagaata
     4201 ccttgggcag tttcccgaga cgccgtatct tggtgatcgt ggctacttac cctgtttcgc
     4261 cccgcgagcg acatacgctg ccatggtgtc acgacttgac cgcgacgtag ggaaaatcct
     4321 cgaggaaatc aagcagttgg gcctggattc cgacaccttg gtgattttta cctccgataa
     4381 cggtccaacc cacggccgag gtggcggctt ggaagacggc gtgggaggct cggattcgct
     4441 ctttttccaa agtgctggtc cattaaatgg gctcaagggc agcgtgtttg aaggagggct
     4501 tcgcgttccc atgattgcac gttggctggg aaagatccca ccggggacgg tctcagatca
     4561 tgtcgccgtt ttctacgatt tcctgccaac atttgcggag attgtttcgg cggatgttcc
     4621 ggcggggatc gacggtgtga gcttccttcc caccctatta gggcggcctg agcaacaaaa
     4681 gacacatgat ttcttggtct gggagttcta tggctatgga gggcaacaag ccgttcgctt
     4741 cggtccctgg aaggccatcc gacgggactg ctacaagtcg ctcgatgggc ccttaatgct
     4801 gttcaacctg gagaatgatc cgggagaaac gcgggatgtc tcatcggaac atcctgaact
     4861 tgtgcagaaa gctgagttga tcatcaagac agaacatcgt gattccccat tctgggattt
     4921 tcccacaaag cggcgtggtc ctcccaaaac gcttgacctg caaactaccc agtgacggtt
     4981 aaagcgttga ggcctgatcg gggtgacggg ccggttcatg ggaagtgctt ttgcgtgaat
     5041 gccgtgagta tatcagtcaa ctagggtttc ccacaaaacc gtgatgatac gtgctgtcct
     5101 ccgcaaggcg gttcgtgatg ggctgccgca actcgttgtg agttgcgtcc tgctgtttct
     5161 tttttcctgg ctgttcgtat ggctgaccag tcttatcaat gtaggtcttt gggcaacgtt
     5221 cttcgacatg ctaccggcgt ttgtgcaaaa actgatgccg gtttcgccaa agattctgat
     5281 aacgcccaca ggtcgcgtga gttttttgtt cgttcacatt gtcccgctac tcatcattat
     5341 tgggtggtcc attgggcgcg catctcagat tgtcagtagg gagattgctt cagggcggta
     5401 tgaagtcttg ttgacactgc ccgttcaccg ctacctttgg gtcgttgtac cggccgtggt
     5461 gatttcgctg ggggccatcc tcatggcagg aagcatgcgg cttggcctgt gggttgcaac
     5521 gatgacggtg cgactaccgg agagactccc gcttgcgcag tttactcctg ggctggtcaa
     5581 tgtggcggca ctctctattg cgctttccgg aattacggcg gcggtttcgg cctttctctc
     5641 caaccgttgg aacacgatcg gcatagccgc cgtgatcctg atggtttcca tgatcgttaa
     5701 gctggttgcc cgtctttggg cccctggcga ttggatgaag tacttgtcgt tcttgaccct
     5761 cttcgaaccc caagagttaa tctttgccgg cggggccaca ggagtggtga ttggctataa
     5821 cgcgggactt atcgccattg gcgtggtggg ctttgtagtc gccgccgcgg ctttaagttt
     5881 acgcgacatt cccgtttctc gctaggatga ggttgctttt gcggggtcac gttctcccgg
     5941 tttgggtaag ctgtacacga tgcgtcgcca tatctgaagg gacgcgcgcc gtggcgtcgg
     6001 ctggttagcg atcgatgtcg ttccgtgtac ggtggccccg acagtgcgtg accctgtatt
     6061 gtggcgtatg attttcagcg ccctaaatgg ccgttcatca ctcgttaaga taggacggtg
     6121 tccatttggc gatgttttcg cagtatagat gccgccaggt ggtcgcagtc tatggcgact
     6181 gttataatgt cggtcacggg ctaggataac ggctcatcac gcgacgagtt ttaaaccgga
     6241 ggaaaaagca ccatgtccga tatcacttca ggccttgtag ctcaggtccc ttggtggagt
     6301 atttggctta tcgtgggaat cattgggggc gttatcctca tcacatttgt cattgttgtg
     6361 ctggcctacg gggcgttgtg gttccaggca tacatgtcga gtgcccgggt tagcatgtgg
     6421 agcttgatcg ggatgagctt tcgtcaggtc aatgcccggc tgattgtcca ggcgaagatt
     6481 atggcggttc aggcaggttt gggcaatgag cgagagacgg ggatttccac gcggcggctg
     6541 gaggcccact atttggcggg gggaaatgtg atgaatgtca tcaaggccct catcgccgcc
     6601 catcgtgccc aaatgcattt gacgttcgat caggcggcag ccattgattt ggcaggccgt
     6661 gacgttctag atgcagtacg cacgagcatc taccccaagg tgatcgattg tcccgatccg
     6721 cgaagtgggc gtacgatgct tagtgcgatt gcaaagaacg gtgtggagtt aaaagtccgt
     6781 gctcgggtaa ctgttcgaac aaagatggag acgcttattg ggggtgctac ggaagagacc
     6841 gttatcgccc gtgtgggtga agggatcatt acggccatcg gctcggccga gacgcacctg
     6901 gaagttttgg agcatcccga tcgcatttcc aaggcagtct tggaacgtgg cctagatcag
     6961 caaacggcgt ttcaaattgt gtccattgac atcgccgaca ttgtcgtcgg ccagaacatc
     7021 ggggcacgac tgcagtatga ccaagccgag gccgataccc gtgtagcccg agcaaaagct
     7081 gaagagcgac gagcactggc catggctcga agtcaggaaa tgaaggccca agttgcctac
     7141 aatcgcgccc aggtcatctt ggcggaggct caggtgccga tagctatggc caaggccatt
     7201 cgagagggaa aatttgaatt gatcccccag cgaaattgaa ggctgtgaaa taggttttcc
     7261 cattcctccg gtgtttgcga aaaacgccgc acggtttggc ccgcgcggcg tttgcttttt
     7321 gatcacgtga tcaggcggaa ggtgtgatca aataggcgtt ccgacaaaag acgtgagcgc
     7381 cttcatataa ttgccacgtt caaacaccgt gggattggga ttgttcttgt ggctcatgct
     7441 gcccttcatt tgttcaatcg actcgtagcc cttttctgcc atccattctt gaatctggtt
     7501 caaaaagtta gtgattgagc cgaacccctg cttatacagg gcagaaacca gcatgactac
     7561 gtccgccccg gcgagaatgg acttcaaaac cgcgttggcc gaatggacac cgctcgttag
     7621 cgccaaggaa gcattaatac ggccatagag gatggcaatc caacgaagtg gtagcaatag
     7681 ttcgaactcg gtgctcagaa caaggtgagg ttttgtttct agcgtttcta agtgaatatc
     7741 cggctggagg aatcgattaa agaggaccac tcccttcgca cccgagttga ccacctgttg
     7801 gacgaaatgg ccgacggagc tgaaataagg gccgattttc acggcgacgg gaatggaaac
     7861 ggacgatgcc acggctttaa ccaggtgaca atactgggac tccacgttgc tggccgactg
     7921 attcaaatcg gcggccacag agtagatgtt taactcaacg gcatcggctc cggcttcctc
     7981 gaacagtttg gcgtaacgaa tccaggcccc ttccgtggtg ccattcaggc tggcgatgac
     8041 agggatcgac aagcttttct tggcctcccg aatcctatcc aagtactcgt ccgttcccag
     8101 gcgatactgc tcaggttcgg gaaaatagct caaggcttct gcaaagctgt cggtaccgaa
     8161 ctcgtggacc ttggcaagat tctcttcttc cgccaaaatc tgctcttcga acagcgatgg
     8221 gagaacgatt gccgccgcac cggcgtcttc caatctcttc tggttatcga gcgactccgt
     8281 aagaggagat gcccccacga caatagggtt ctttaatttt agaccgagat aactcgtgcg
     8341 caaatcaaca gacatactct gatcctccca gaggcctctt cctaagtttc atcactgacc
     8401 atcaccacct tcgggctttg caccggcgcg tgccaaggcc gaactcaggt tgtcgtacaa
     8461 cgcatatcgg aagttaatgt cctcttgccc taattggaag agcgtctcgg cccgttctgg
     8521 cttggaccga ctcaagatgg cgaagcgtgc ctgtttcaac gcgaaatctt tgaacgctcc
     8581 cttaggcggt cggctgtcca cgtgcaaagg ctgttccttg tcacgaggat cataatggta
     8641 gagtggccaa tatccgcaag cgacggcctc tttttggtat tccatgccct tgaccatatc
     8701 gatgccccag ccgatgcagt gcgaaaaagc gataatcaac gacgggcctc gataagattc
     8761 cgccgcgcgg atgaccttga tggcatgcag gggattggcg cccaacgaga tttgacccac
     8821 gaacacgttg ccgtaggaca tggccatcaa accaaggtcc ttctttcgcc cctgttttcc
     8881 tgcggcggca aacttggcca ctgccccgcg gaaggtggct ttcgacgcct ggccgcccgt
     8941 gttggaataa acctcggtat ctaagacaag caggttaatg tcccgcccgg acgctaagac
     9001 gtggtctaat ccaccaaaac cgatatcata ggcccaacca tccccaccaa agctccaaat
     9061 acttcggcgg atgagatagt cagcggaggc gaggagattc gcggcgtcat ctccagggat
     9121 ggttgccagt ttcttcttca attcggcgac gagctggcgt tgcttcgcga tctgctcatc
     9181 agtttcctgg acattattga gcaactcggt aacaagcgta tccccaatct gcggggccaa
     9241 tttcttgaga agtcctgtgg cgtattcgat ttgctggtcc acagccaatc gcattccgaa
     9301 gccaaattcg gctgcgtctt caaagagaga gttgttccag gtggggcctt tgccctcggc
     9361 attggttgtg tacggggtgc acggcaagtt tccaccataa atcgacgaac agccagtggc
     9421 gttggcgatc atcatgcgat cgccgaagaa ttgcgtgatc agtttgacat acggtgtctc
     9481 gccgcaaccg gcgcaagctc ctgagaactc gaacaacggg agtaagagct gcgacccctt
     9541 gaccgtatcg acggccacct tggaacgatc gagatccgga atcttgagga agaaatcgaa
     9601 acaagctcgt tcgcgatcga gatggtccag cttaaagcac atatcgatgg ccttgtgctt
     9661 ggccacttct ttgtcgcgaa ccggacacac ctcgacacac aatccacagc cggtgcaatc
     9721 gtccggtgcc acctgaacag tcacgtgata gcccggatac tctttgcctc gccacggtgc
     9781 ggacttgaat ccttccggtg cgttggccag aacggcggga tcatatgcct tcatgcgaat
     9841 agccgcatgt gggcaaacga gggaacacaa accacactgg atgcaaatct tggggtccca
     9901 aatgggaatt tccagggcaa ttgaacgttt ttcgtacttt gtggtgcctg tcgggaaggt
     9961 gccatcaacg ggcagcgcgc tgacaggcaa atcgtcccca cggtaagcaa tcatcggccc
    10021 cagaacgttc ttcacaaatg gtggcgcatc ttcgggcacc ggcgggacca tgtggatatt
    10081 ggacgtgacc cgatctggta ctttcacttc ccgcagtgat tcaagggcct tgtcaacggc
    10141 ggcaaagttc ttttcgataa ttgccgaccc gccctttttg ccgtaggact tgacgatctg
    10201 atccttgatc tctttgatgg cctgttcgac tggaatgacg ttggccaagg cgaagaaaca
    10261 cgtctgcatc acggtgttga tgcgcacacc caggcccgtc tccttcgcca cacgataagc
    10321 atctacgacg taaaacttta gtttcttgtc gataatctgc ttttgtacct ctgccgggag
    10381 atggtcccaa acctcttctg cggggaacgg ggaattgagc aggaatgtgg ccccttcctc
    10441 cgcggccgac agcatgtcga ttcgttccag gaaatgaaat tgatggcacg cgataaagtt
    10501 ggccttgttg atcaggtagg accccttaat cggccgtggg ctaaagcgca cgtgggaaat
    10561 cgtcatcgac ccggctttcc gggagtcgta aacgaaatac ccttgggcgt aaaacggagt
    10621 gttctcgcca atgattttca cagaactacg atttgcagaa acggtcccat cgcttcccag
    10681 cccgtagaat aacgcccgga ccacatcggg ccgttctatg gagaaggttg ggtcatagct
    10741 caagcttaga tgggtgacat cgtcattaat gccaacggtg atgcgccgct tgggctgagg
    10801 ctttttcaat tcctcaaaca cggccagggc catcgcagga ttgaactcct tcgacgagag
    10861 gccataccga ccaccaataa tcagcggcaa ctcggttccc agggcgtgat cccgccaccg
    10921 ttcgttaatt gcggccacga cgtccaagaa tagtggctca cccaaggcac ccggctcctt
    10981 ggttcggtcc atcacagcga tccgtttgac agttttagga agcactcgga cgagtgcgtc
    11041 ggcatcgaac gggcgataaa ggcgaacttt caccatgccc accttttccc cgcgttccac
    11101 taagtaatca acagcttcct ccaccacccc ggttccagaa gccataatga cgacgacccg
    11161 gtctgcgtcc ggcgccccga cataatcaaa gaggtgatac tgtcggccaa agagcttggc
    11221 gaacttgtcc attgcctttt ggacgatcgc cggcgtcttg tcataaaagg gattacaggc
    11281 ctctcttgcc tggaaaaaga cgtctgggtt ttgagccgta cccctgagga cgggcttttc
    11341 gggcgacatt gctcgttcgc ggtgggcccg gaccaattcc atgtcgatca tggcccggac
    11401 gtcatcgtcc gaaagttgtt cgattttggc tacctcgtgg gaggtccgaa atccgtcata
    11461 gaagtggata aagggtaccc gcgattcaag cgtggcggcg taagcaataa gcgccaaatc
    11521 ttgcgcttcc tggacagagg ccgaggctaa caaggcccag ccggtactgc gacaggccat
    11581 gacgtcgctg tgatcaccga agatggaaag ggcatgggtg gccaccgtcc gtgctgtgac
    11641 atgaaagacg ctgggtgtta gttccccggc gatcttgaac aaattgggaa tcatcagcag
    11701 gagcccctgg gacgctgtaa acgtcgaaga gagagctccg ccctggagtg caccatggac
    11761 ggccccggca gcacccgcct cgctttgcat ctcgatcacg agcggaacag caccgaagat
    11821 attctttcgc cctgctgcgg cccaagcatc aggtaattcg ccaaggctcg aggatggcgt
    11881 gatgggatag atcgccatca cttccgagca aagatgtgca atattcgctg ccgcttcatt
    11941 gccctccaca gtcacgaagc gttgctgtgt cacggctaag tctccttcaa aaaaacgcct
    12001 gtcacatatt cgtaatcatc ggcagccgca ctcattcact cagcccaagg tcaggacgaa
    12061 cgcggtcaac cgacgtgtca aacttcactc cgtaattctc gtacccaaca gtgacaaaag
    12121 tccgcttaca agaattgata tacgcaccca ccgactagga tggttcgccg tacccaaagc
    12181 cgcagactca ctcgtcccgg ctatattaac ccgatagcct aacgcttttc caggccattc
    12241 gcaagtcgtg cagtaacaga atgattcgta tttggagcct atcccaaaac tccctctccc
    12301 tctgggagag ggttggggtg agggcaagcg accgttggag tcgatcgaaa aaaccggtag
    12361 cgatcggagc cacccttacc gccagcccct ctcccgccga acgggcgagg agaggttggg
    12421 ggataggctc ttagcagtta ctggtgaaca gtgtcgccag agattcattc cggccgacaa
    12481 cagttctttg gaaccgttgc gagatgctct ttttggtttt ctcccctcat ggaacgggcg
    12541 tttattctct ctgacagatc ttggcagatt ctagcggtgg gtagactttc ggcgacatcg
    12601 ccataaaatg acatcggtag ttgtcgtaaa taagtctcaa ccagttggtg ccgtatccaa
    12661 acgacgattc gaggtgaagc catgtcgcta ttgaagacac ttgcaggcat cgttgttgtc
    12721 gctgtgaatc tcgtttccca aaaacttgcc ccacgggaac agacgcctgt tgagctccgc
    12781 gtcggtgttg tcgccccgga ctttgaactc ccgggcagtg acgggaaaac ctaccgtctg
    12841 gccgactttc gtggccagaa gatcgtggtc ctggcctggt ttccgaaagc ctttacgccg
    12901 ggatgtaccc gtgagtgccg cgtttttgcc gagcgggccg acgagttcgc caatttacct
    12961 gtcgcatttt ttacagccag tgtcgataca ccttcggaaa acaaaaaatt tgccgagtct
    13021 gtcggtgctc gttatcccat tttgagtgac ccagaccgaa aggtcgcgct ggcctatggg
    13081 gttgttagtt caccgcggga cttcgcgcgt cgttggacat ttattatcgg cctcgatggg
    13141 aagattcttc acattgataa gaacgtcaag gtcgataccc acgccagcga tgttgcccgg
    13201 tggataaaag aatacttgaa ggccaatccc ccggcagggg attcaggcaa acggtgaatt
    13261 tcacaccgga actctttctg ggcgatccac ctctgggtgc gtgttggcga gatgagcacg
    13321 ggcccatcat aagcagactc cccaggttgg acgcccgggg agtctgatga acagcaaacc
    13381 acacggattg tgcggattgc ctccaatatg gctgcttgct acccgtctgc caattctgtc
    13441 acctttaccg cttcaataag cggagccagt tcaggtccat ctcaaaatcg acgaccatct
    13501 gctggcttgt gaaatcgacc ttcttccgca agagctgaac accttcgcac tgctcaaggt
    13561 tagccttgag tgtttggagc cggctatctt gcggattcag tttagcggcg gtggcgacca
    13621 agtcgtcaac cttgagacat agctcgaagg ccggggccgt ttgatcgccc tgagacacgg
    13681 cgtcatcaat ggcccgtttg atgacatcaa cggggttttt gccgatggcc acggccgcat
    13741 agccgttgct cgctgtgaag gcgacctcaa gatcatctcc taagaccgcc ttttgctcgg
    13801 gggaaagcct gtcgccaagg gcaatccgca taaggtggat gctggcgtcc ccatgtagcg
    13861 tgtgatcggg gatgatcgtg actctctcaa aggccttggg ggcgcgctgc tgtgccgatt
    13921 cgactacgtt acccacccag gcctcaaggc ccgtggagtt tcccacatac ccgccaatca
    13981 acagcgttac ttggtccggt ttggtaacca gtgtcacgat gccatcggct ttgccttcac
    14041 gtgcccaatc gccgagaagc cccaaaaaat cgtgggccag atttttggcc acctgtgcgt
    14101 ccacggaatt agatatgcga cgctcaattt gcttatccca gcggctctga atgaggcttc
    14161 caatccgctc gaggtcttcc tcggatgggg gtgggaaggt caccgaccaa gccatcgtca
    14221 tggccgtgtc gttccaaagc cggatagcaa ccagtgggct agtaacaagt ctttcttggg
    14281 cgaactgtgc ggccaaatca gaacctgcct tggccgtcca tgtgcagttt cccacgagtt
    14341 tcttctcatt tcgatcgatt cgataggaca agatcacctt ctcggtttgt tgaaccgctt
    14401 cctttgcagt ttcaaggagc ggccgaagga cgctttgccg aacctcttga gaaacttcaa
    14461 ttctcgagag catcgcggcg agcgcttctt gccgttcgag ccaattccta aaaactcccc
    14521 tcagcgacgc cagttcgcgg gggttgatgg cgatcgacac atccatggaa gagtcgaatt
    14581 cggccagcca cattgcggga tctcccgata gcgagctgag gatttccgcg ttatggctta
    14641 cgacgaccca ggcatcgtaa agcctgacgt agagctctcg gtctcgaacc ttcacgtggt
    14701 aaacgttatc cgagatcaat gtcggttcgc tgatccagtc cttggcgaaa gtatgaagct
    14761 gatccaaatt tgaggtggga atgcacccaa ccaggatggg gttgccttgt tcaccgacaa
    14821 gacacgccaa gcccatcggt ctggtgggat caacaccgtt cttccccacg gcgtcaagga
    14881 ggcccacaag catctggatt ctgacactgc ttgggtgctc tttgagcatg tccgccaaaa
    14941 acgcggcgtt ctgctgccac tgggcgagag gggctagtcg cacaacgacg gccggtgccc
    15001 cagccaagac gggcagggcg ctgaaaacga ccgccagtcc ggcgattagg cctaagctct
    15061 tggttagcca caaaggtcgt ttcactcttg tcattcctcg tctcctcctc caattgtgga
    15121 cccacgaaca tctacctgta acaacgctgg ctaccaatac ccctttcaaa gagtggctgg
    15181 cctacagtta aggagtctcc acctgagaac tttccgcacg ggagagaaca ccccaaggaa
    15241 gttgttggtg aagaagttcc cccgccagcc gaaggtcatt gtccaaattg gcccaacgct
    15301 gacggtcgat ttcgctgagg gcgatttgga cctgctggat ggtgcggtca ggggccgaga
    15361 ccaaatcctc gatggcctca gtaggcggtt ttccgtgcac gaggttcaca tcgctcccgc
    15421 tgcccttttc ttgccggggc tcattcaggt tctccccggc tattcctgaa acctctatat
    15481 gttggcggtg aaaccatcca gacctttgaa caacctgcat caggatggct acggtcacca
    15541 cgctcgcata cgtcgccaag actacccagg caatctgctt gaaggaaagc cgcgatttac
    15601 cggaggaaaa caaatgagcg cgctcagcac caggtatgcc gacctggttc agaatccgcc
    15661 cgtaaaggtc ctccgaaaac gcgggtcgtt cagcctctgc ttctcgtttg agaagctcgg
    15721 ctaacggctc ccgtctcaga ttctgttcgt caattccatg atggagcgtc atgattgact
    15781 caccctactg atgatcccag gagttacctt ttgtttgccc tataaactgg gtcaattcgt
    15841 gacttcgatg caccacgaca gcgggtaaca aattgttttc taggtaatgt gcccgttagt
    15901 gcctttccgg tgaaacggcc tcatcttcgg agacgggcaa cgtgttaaac tttgcataag
    15961 cgcgtaatcg atgtacgagt acctttcttg cgcgacagag gatcgttttt acggcacttt
    16021 ctgattttcc gatcactgcc gcgatttgtc ggatactcag ttcttccaca taaaatagcc
    16081 acacggcggt aaactggatc gggctcaatt cggcctgaac cgcatcccac aagcggtgat
    16141 tttcttccgc atcctccaat aaagaatcaa cctgagtgat gcggctgctc ttttcgtttt
    16201 cccccaccgt ggaataccga tggcctcgca tcccgttcaa agccactcgg taagcgatgg
    16261 taaaaaccca cggccgaaag ggtgcaccaa cgcgataatg cgacagtttt cgaaagatgc
    16321 gaaggaacgt ctcctgcgtt aagtcctcgg catctgttga tgaacgacac ttccgttgca
    16381 agaaatgcag caccggcact tggtaccggc gtaccaactc ggcaaaactg gctaagcagc
    16441 ccatttgggc ttgctcggca agttgctcat cggtcaaaga tgtcaatgag gccaagtgtg
    16501 acatcttgct cgtcgagtta ttggcggcac agacacctag gtgcaatgac ttttgtcacg
    16561 cggaccacgg tattcgatgg ttcgcaaccg ccatttgggc atttcttcca tactgtccca
    16621 gttttctaaa cattgccttg aaaacgtacg caattacgtg attaaggcag tcccccagat
    16681 tttttacctt ccgtagctac ttgagatgca atctccttaa ggacccgcaa ggcaggttct
    16741 gctggcatcg tccagaccat ggtggtcttg ttttcggagg caaaaacacg tatgagcgtc
    16801 aaacccgcca catattcagg ccgatcggca taatgtagtt tcccaatcgc aacgagtccg
    16861 gtgagcgcat cttttagatc taaagcaatc tctgccccct caacttcaaa ggaacactga
    16921 aggaagaact gtcctgactg ttccccaacc atggccgata atcggcgact gcgacggagc
    16981 agcggcgaca taaaggggac tgatagttct tgcaactcat tggcatgaat ctgcaagtac
    17041 gtacccttgc gaacgttgaa aaaatcgggc ctttcctgag acgatttttg ttggtaccgc
    17101 tcgatgatca cctcaaccgc cgtcgcatct tttccgagca caagatggtc ttggtcgaga
    17161 aagcagcctg tgatctcgct gttgtcgtta cggtctgtcc actggtgaag gataaggcct
    17221 tttgattctc gtcttcgata cgtcggctcg ctcgcaatca gacccaccaa taaatcagga
    17281 ttcatccgac agtgaatgat catggcaccg aatttgggat cgaagcgtgt ccccaaaaag
    17341 gtcacgccgt gaaggtcttt cttcaaattg aggccggcca gcgcggcaat ggcttcaatc
    17401 ttgtgtcgct ccggatgcgc gtgccaaaac tcggaaaaaa gagtggctgc taccttggaa
    17461 tcacggagct ggtcaatgtc aaagtgaaca atccatcgcc agttgtccgg gacctgtnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca atggcggtgt cgtgaatcgg
    17641 tttgtttttt ggtcgtgtct tgatcaacgt aaaggagcca gtatgccaaa ccgaagggaa
    17701 ttcctgggca tgggttgtgg tcttgcgatg agtgggcttt tgggctcggc ggggacactg
    17761 ctttgtccga agcatggatc ggcttccaca ggatcggaag gcgaagaaac cattcggaca
    17821 gatttcctcg acggctcatc aaaatcggtg gcctggctgg aggaaatggt aggaccctca
    17881 cttgggcgac ttttgacaaa aacgtctcga gaaatccgca gttcgccctt ttctgtaggg
    17941 ctggaaacgc tcgaccgatc catgtacgat cccgaacggg tttatgatcc cttgggaaaa
    18001 ttaggagtca aatgggcaag gctgcaaacc ggctgggcac gaaccgaaca aattgttgga
    18061 caatatgatt ttggctggct cgatgccgtc gttgatgccg tggttgggca aggcgtagcg
    18121 ccatggttta acgtgggcta cggcaacaaa ctatataccc ctgaggcgcc cgacgcctcg
    18181 gccgtgggtt ggattccaat ctacaacgcg gacgcccagc aggcctggtt gcggtacgtt
    18241 cgcgcgctgg cccagcgatt cggcgaacgg gtcaaacact gggaaatttg gaatgagccg
    18301 aatatcaaga acttttggaa accgggtgag ccctcccccg agctttatgt cgagtttgtt
    18361 gcccaaacgg ccaaggtcat tcgagaggtc atcccgcagg tgaccatcat cgggggtgcc
    18421 ttggccggga tgccgacgga ttatttgaaa aagctattgt cctgcggttt ggcccaatat
    18481 gtggataaga tttccttcca cccttatcgg gctattcctg aggatggcta ccgggacacc
    18541 ctggagacat ggaagcaact tttgcagagc gctggcgaga agcgtctcca attgtggcaa
    18601 ggggaaaacg gttgcccttc tcgaccggga agcgcggggg ctttggcgca gtttccttgg
    18661 aatgagatca cacagtgcaa atggctatta cggcgcgtcc tctatgaccg ggttttggga
    18721 ctggaattga catcatattt tcacatggtc gatttgatgc ggtacaactg gggccaagga
    18781 ccgacggata aagcaaattg gaaaggactg ctgcgcggtg aggattattc gcccaagccc
    18841 gcctatttcg gctatcagcg aatctgcacg cttttcgatg acgacgcacg acgtgacata
    18901 agtctagata ccagtgttga ggtcgtcgag gagaataggg ccgaccccgc aagttcgcgg
    18961 tcgtccaagg aaaaacgatt cattccaccg gccgcttttg ttcgtaacaa ttctccgatg
    19021 tggtgtttct ggtacgccag tgatttgatc aaacccggcg agataaccta cctgacccta
    19081 acgatccggg aaaaagcgct ttcgagcggg gaattgttct acctggacct gctaagcggt
    19141 tggatcttcc gcttgtcaaa acttgaacgc cagaatgacc agtgccatgt tcgaagtatt
    19201 cccgtcgggg attatccggt ggtgatcgga gcgccggggg ctttacaagg tctcgtttcc
    19261 tcgtgaccgt tgcctcagaa atggtgaggt ggaacgccga accaaagacg ggcacggctc
    19321 caagaccagg cttgacgagc gagcgtcctc gacgaggtga tgtggtcgcg ggaaaccgaa
    19381 cgggatcata gcacactttg ttcccttcca tttgatgaaa gtagccaaaa aagataggga
    19441 cacaacctga tgaggctgtg tccctttctc gtcgttgtac gaaatcaggc tgctgccggt
    19501 tgcctcccca gcgtcgaaat cagccgccag cgggaggcgg cgtggtttcc gctggggcgg
    19561 gaggggctgc gggagccttc ttcgccgccg gcttcttggc cggttttttg gccggcttct
    19621 tcgcgacctt cttagcggct ttcttcaccg ccttctttgc cggcttcttg gctgctttct
    19681 ttgccggctt cttcgcagcc ttcttggcgg ctttctttgc cgnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nngctttctt tgccggcttc ttcgcagcct tcttggcggc
    19861 tttctttgcc gccttcttca caacttgctt ggccaccttc aaaacctcct tactgaagga
    19921 caccctggga gccgttgtgg aagacatatg ccacgtaact gatccacaac gcgcacccta
    19981 tcacaacacc aactaccacg tgccggtttc cgcacctaag cggattgcac ctaagtcaac
    20041 gtccgtcttt gcttgcttag ctgctgccgc gtttttcaca gcagcagcct aaaaatatgt
    20101 taacgttttc tttcaaaagt gcaagttgaa tctcaagctc aaatgggcaa attgatcggg
    20161 tgatcagatg cccattgtct tcatccgtcc gtggcagtgc tatacagcgt tcgctttaag
    20221 ccgggatttc tatccagcgg ccctcgcggg cactctggag cactgcgtca caaacgcgct
    20281 gtgtccgcag tgccgaccgc atatccggct ggaactttgg tccgccttcc agcgacgcga
    20341 gaaaatcggc aaacgaattc gtaaatgtgt gttcgtagcc aatcgtacat ccgggaaccc
    20401 accagtgctt catataggga tgctcactgt gggtcacatg gattctttgc cagcctgtca
    20461 aatgatcttc gatcttcttt ccggtttctg ggtcggcata gcggaagaac tgcaaaatgt
    20521 gcggatcttc caggtcaaag aacacagaac caagttctcc gttcaactca aaggtattgt
    20581 agttcttgcg accccgagca taccgcgtgc tctcgaaagt gccgagtgac ccattttcga
    20641 agacagccag gaacatgcag gcatcgtcga tgttcacctt ttcgactttg cccgtttcgg
    20701 catgcacccg ttccttcacg aaggtctcgg tataggcaac aaccttcttg attgggccgt
    20761 tgagccactc agccgtatcg atcgagtgtg caagcaagtc gccggtcacg cccgacccag
    20821 caatgcgagc atcgagtcgc caaagggcca aaccgccctg cgggaccttt gtggagatcg
    20881 tgtaatcctg gttataggtc gcccgatagt ggaaaggccg cccgatacgg ccttcttcaa
    20941 taatttgctt cgccaaggca atcgctggga cgcggcggta attgaaggag accatatttg
    21001 ggacgccagc cttctcaacg gccgcgacca tctcctcggc ttgctgggca ttcatagcca
    21061 gcggcttctc acagacgata atcttaccag cctgggccgc cgctataacg cagtcatagt
    21121 gcatgtggtt gggtacgcac acatcgacga ggtcaatgtc gggtcgttct atcaaccgcc
    21181 gccagtcggt ttcgatcgac tcgtagcccc agcgtttggc aaaggtctct aattgctgac
    21241 ggtcttcctc ccggccatag caggctttca aaacgggctt atattctcga tcaaagaaat
    21301 gagacacctg gagccaggcg ttcgaatgag ccctgcccat gaacccgcaa cccaacatgc
    21361 cgatccgaag ttcctttgcc atgattcgtt ctcctttacc aagtgactcg tgatataccg
    21421 atatgcccaa caaaaatccg gtaaaacaac atcacgcggc ttcttcttca taccaaccgt
    21481 gctgatcgcg cacctggatc atcacccgca agatgtcatt ccaggtttga ggattcatca
    21541 taaccgcatt gggaaacata caaccatccc agcagatatg tcgcatccgt ttcgttacat
    21601 taccgttttc atctcttagc cagaaccctg ctgcccgggg gatgtccaat tttccattgg
    21661 gatcagtcgc caaacaatgc cgaccggtct tatcgtgtga acccgatcca tggaccgtcg
    21721 catcattttg ggcaatatgt aggtcgattg tccaggggcg cagcgcatcc gttaactgct
    21781 tatacgcggc gtaaaaccgt tccgaatcct tccagtcgaa atcttggggc aggatagcgt
    21841 cttcgggcgc gttgtaaccg agaaggtaca acagtgtgtg ggccatatcg gcctgaaagc
    21901 ccagcacgtt gggacgatcc accatttcca gaagctggat catccgacgc caactgtgca
    21961 tgccacccca acagatttcg ccctctgcgg ccagtttttc gccatgatcg gccgcgatat
    22021 cgcaggcctg gcgaaatgtc tccgcaatcc gccgctgatt ggcctcgggg tcactcagcc
    22081 aatcgctgac actgcaagcc gaatcaatcc gcacacaccc gtaagggcga acgcccagct
    22141 ctcgcaagac catggccacc cgacaacctt tgcggacctg atcgaggaac tttcgacgtt
    22201 cgctctcgtc ccccatggcg gaaccaccac ctgtcggggg ccaaataggt gcaacgacag
    22261 ttccgatctt caggtttcgt tttgtcgcct tgtccgccag ttgcttgatg gcatcatcgc
    22321 tcttgaccgt ggagtcaata tcgatatgcg gtaaatagag gaagagatca aagccctcaa
    22381 acttgatccc atcgacctcc gcggctgccg tcaagtccag catcgtgtcg agatcgatgg
    22441 gaggttcgcc accatcgccc tttccaacga cccctggcca ggctgcgttg tgcagtttag
    22501 gaaagttgtt acgcgttgcc atcgctcgcc gtcctttctt tgctccaggt aatcaccggg
    22561 atctctcatg accatcggcg tactttcctt ttggtcaagt cgcgtacgat tctaagcctt
    22621 cctacgaaag gttacaagtc accgcaattg acttttgcga ttccagagtg tcgaatacta
    22681 ataaggggaa ggcaaagccg ctgaagatgg tttccgggct tttccaacgg gccagcacga
    22741 ctcttgagtg ataggagaca taccacatgg ctcaaggttt gataggcccg ttacacacag
    22801 cgtccctaaa tctcaaccgg cgaggctctt tcatgaaaag acgatccggt ttcaccctcg
    22861 tagaaatctt ggttgccaca gcgatttcgc tcattatgct aggagctgtg gtagcggtct
    22921 ttgcctcggt catgcaaagt atcaatcaag cgagggctgt tctggaaacg tcagatcggc
    22981 tgcgatcggc ggcggagctg cttcgggcgg acctcaaagg gttgaccgtg atccccgtgc
    23041 caccgcgcga cccggcaaat aacgaggggt atttcgaata cacggaaggg ccgattgggc
    23101 aaaatggtca gatccgacca tttgcggggg cccatccctt ggccgttgtg aatcttgatg
    23161 acttggacgc cgccggaaac ccctaccaag attcgaccgt tggcgataac gatgatattc
    23221 tcatgttcac cacgcgttcc accggccgac catttgtggg gcgttgtttg gtgagtggcc
    23281 agcccacgac tattgaatcg gatgtcgccg aagttgcctg gttcgtccgc ggaaaccgtt
    23341 tgtatcgccg gttgcgaatt gtcgccccta aagcgatggc gctatgtgat gtcaacgcaa
    23401 atggacgtct tgatcccgca gaatggatcc ctgctaacga taccggagcc gtgaatttca
    23461 accgctatta cgatgtggcg gtccatttcg acaggaatgc cggcggttgg gtcttttcca
    23521 cgctgggcga tctcacaaaa cccgagaatc gttacgccca cttgataggt gatccattga
    23581 atcctgcaag ctttccgttc catccccacc gggcgggatg gcagcccttg ggtttgccaa
    23641 cgttagcgga aagtgcttcc gcaggggcgg cgacgacgtg gctaactagc ggcctattgc
    23701 cggctctcag cttaacatca ttggcggcac ctctggataa gtgggacctc tggacgaatc
    23761 cctatcccgt tgctgaggtg gatccccaga cagggcttct gcgaacggtg gtggatcccc
    23821 agacagggct tttgcgaacg ctcgagcaac tttaccgcga gtcggaagac cttgtcttaa
    23881 cgaacgtcgt ggcttttgac gtgaagatct gggatcccac ggcgccgatc ggtgcggtgc
    23941 agacgggagg tgtcgtccgc ccgggtgata taggcttttt tgacctggcg ttccaccaaa
    24001 ccgatgatcg tggagtcttt gatcagaaat tcccatacgt tggggcttat gtcgatttgg
    24061 cgtatgcgcc gacaatagaa aaactttcta cattctcggg tttcccgctt gctaagacgc
    24121 ggttagcgtc ggcgggcttg gcggtctacg atacttggag ctcccattac gaaaaagatg
    24181 ggatcgatca ggatggagtc ggtggtgttg acacgggcac tgacggtttt gacaacgaca
    24241 atgacggctt ggtggatgag ttcgacgaa
//
LOCUS       DAOSUT010000056        24126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1370_length_24126_cov_9.977857, whole genome shotgun sequence.
ACCESSION   DAOSUT010000056 DAOSUT010000000
VERSION     DAOSUT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 24126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24126
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_24126_cov_9.977857"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PLD05_06940"
     CDS             complement(<1..944)
                     /locus_tag="PLD05_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP77208.1"
                     /translation="MTPILMFLGFSLPFVALLATAAAWGVYPSLRAVLLGLIPVTATL
                     GLVVLESAWVAVLIVDVAYLVIIIRDLFTVPRKNWLRCERNHAKVASLKKRHPVSLTI
                     TSLAPWMVKAVIRDGADRILQPTPPLIHVALRPGLRTVVDYELRPQRRGAFSMSAVYI
                     RGNSRYGLWFRQMTYPCETVLHVYPDIKQLSEYAMLARVNRLSLIGVRRVRRAGHEDE
                     FERLRDYTIDDEYRWIDWRATARRRKLTVKDFQQSQSQRVVFMVDCGRMMTNEWAGLS
                     MLDHTWNAVLLLAYVALKHGDSVGLLAFSDEIHAFLNPR"
     gene            complement(941..2176)
                     /locus_tag="PLD05_06945"
     CDS             complement(941..2176)
                     /locus_tag="PLD05_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP77209.1"
                     /translation="MRLWYYAKGPNRFGPIEEAELRRRLGTGEIAAEDWLWTEGMSNW
                     VKAAEWLRTAGDPVVEKPIVAEAVAGPSAAVVVTASPPPVNDLAAGSLSQRMLPVREL
                     YERIEREVRKVFVGQDELVLGTLTALFSGGHVLIESVPGLGKTLFVRTLGRVLGCKFN
                     RIQFTADLMPSDITGSPIFDVKTQEFIFRPGPVFTNILLADEINRSPAKTHAALLEVM
                     QELSVTVERTRHKLDRPFMVLATQNPVESEGTYNLPEAQMDRFMFKLIMDYPREEEEA
                     RILILHSVREDIDQRLQELSVVTNPEEIMSVTRLAGEVRVDPKLVNYINALVRKTRNW
                     PQILVGASPRAGLALMQAARTLAAFRGRDYAVPDDVVDLALPALRHRVILAPEAEVEG
                     YRVDDVLHEVIRSVEVPRL"
     gene            2560..3399
                     /locus_tag="PLD05_06950"
     CDS             2560..3399
                     /locus_tag="PLD05_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77210.1"
                     /translation="MWRSILRQWAQQTAAEAFQARVEEMAHETAQPAADSPPRPIDVG
                     IVFALASESGGLEDRLEEKIWIRGEAGQVVIGRAGQKNVAIIVTGPGAKSAHVGTEAL
                     ILGHHPRWIVAAGFAGSLNEHVRPMELIIPNRITDLTGRALQVGLTLDADQTKGIHVG
                     PLLSVPELVRHPLERRKLAEEHHAVAVDLESFAVAEVCRRYQVPMWVVKIVTDGVDDQ
                     LPPEVSYLMKPRKWTEKLGAVAGALWNRPGSIRDLWQLNELALRASDRLAEFLLSMIR
                     SLP"
     gene            complement(3848..4999)
                     /locus_tag="PLD05_06955"
     CDS             complement(3848..4999)
                     /locus_tag="PLD05_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOP77211.1"
                     /translation="MIHLTQKGCRAVDTGAAQILVPGVVFLPGLGLDRSAGRVEPGSF
                     IAGEENWVALAAVQRMIEPHPPSCFTLFGPTGTGKTHLLGLLCGLWKEIWPHCSVVSS
                     TAAEFGQAFAEALVSRKARQFRRHWEEADFIVIDDLQFLGDYPAAQEEFVALLRAAER
                     GTLCVAVACRIPPWHCENLNPRIGSILQGGLTARLQPPGEDARRAILQMAFRQKGVTV
                     TAAPLQLFAEELAGSPRQLLGFALKLRQAAGRGPVDYPAARAFLRRYSPRPATSIDRV
                     AKATARYFGIRLKDLRGPGRSRAEVLARNVAMYLAQRLTGASLQQIGHYFGGRDHTTV
                     GHGCRKIEALSRSDPSMQQAIFMIEERLIPGEPQTICSAADETHPSRRS"
     gene            5400..6956
                     /gene="amrB"
                     /locus_tag="PLD05_06960"
     CDS             5400..6956
                     /gene="amrB"
                     /locus_tag="PLD05_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOP77212.1"
                     /translation="MNARLSDRIRPPAVAGTFYPAQRERLREMLDQFLAEAPVICGPD
                     RTIRAIIAPHAGYRYSGPVAGVAYKQLAGRDYNTVVLLGPSHFAHFEGGALPDVDAFA
                     TPLGEVAIANKARILAQKEPYAFDPPAVVQAPRGWTTTGTSACTPHTFEHSLEVQLPF
                     LQVILKEFEIVPIVFGRVNVNQAAEALASILDDRTLIVVSSDLSHYQPYRRACSVDQR
                     TLQAIIQMDFDYLEGLRYEPELCPCGHTGILVLLRLASRFQWEPILLDYRNSGDTSGD
                     RSAVVGYGAVAFCEGRARSVAKANEEEVKAEEINPVASGAVSASGDTEEAPSEARLPQ
                     LTAEEREFLLNLAWKTIRRYVIDRRLPEVEPESVPPRLREPWACFVTLRRRGALRGCI
                     GTLTAERPLYLAVMENARNAARDPRFPPLEDWELTDLTVEISVLSEKRPLDARSPEEI
                     LRKLRPGIDGVILERFGARATFLPQVWEDLPDPVEFLQRLSQKAGLPADAWRQPATRI
                     FTYEVEKFGD"
     gene            7166..7633
                     /locus_tag="PLD05_06965"
     CDS             7166..7633
                     /locus_tag="PLD05_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77213.1"
                     /translation="MRLWMPGVVVLSVILIGGCCAPCHRLGMHHYAAPGAVYGEEPGV
                     VAYHAGPLAWLWRLLGVGRGYPCDDCGPRYWGDWGGDRAGCEPCDDYGSWTGMPPVEY
                     RGAAGTYSSSGDKRGGCPECQSSSSSGSTVSQINPRTGRTSVAVQSTKESSTR"
     gene            complement(7681..8829)
                     /locus_tag="PLD05_06970"
     CDS             complement(7681..8829)
                     /locus_tag="PLD05_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77214.1"
                     /translation="MRSQQRDSLGAFLSDEYYVLTVPNGEQALRILPQAAPDVVLLDI
                     MMPGLNGYETCRQIKSGPLGPFTQVILVSGKSSTQERLEGYAAGADDYIVKPFDHEEL
                     LAKIGVHLRLKKMVERVWDLNMQLHRFTDNLEALVSQRTAEILETRDLLIFSLAKLAE
                     SRDPETGEHLERMRWYSRLIAEEYIASRPHGDSESENFPQEVLRSAPLHDIGKVGIPD
                     VILLKPGRLSPEESVIMQRHTIIGYEALMAACNKSKAGSFLEIAADIARSHHERYDGK
                     GYPDGLAGNDIPLAARIVSVADVFDALTSPRVYKRAWPPDVARATIERGSGTQFDPDV
                     VAAFLRRWRAIEEIGAPFWNTIPPDRLEPHSLIQTSAPPLLTASLATV"
     gene            9352..10758
                     /locus_tag="PLD05_06975"
     CDS             9352..10758
                     /locus_tag="PLD05_06975"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOP77215.1"
                     /translation="MSGSQRVIHQYWSVRELFYHVLDGAVIVGALWCARLTLGGFREE
                     DLLAALLAVIVFHVVAEITGLYRNWRGVSLERELLCAAITWFFTLPALVTLGYFVDHG
                     RIVSRETVLVWAGMGFLLLLVSRSAVRWVLNRLRASGYNTHYYAVVGINELAFRLVES
                     LENSPDLGLKLYGFYDDRPESRTGPVPEEIGQKLGNIEELVEHARQRRVDMIYITFPM
                     RAEDRIRKILAQLSDTTASVYIVPDFFVFQLLHSRWTNIGGLPVVSIYENPFYGIDGF
                     VKRAMDVVLASIFLVLLSPLMVAIAIAIKLTSPGPVFFRQRRYGLDGREIRVWKFRTM
                     TVCEDGQQVTQARRGDPRVTRIGAFLRRTSLDELPQLFNVLKGDMSLVGPRPHATAHN
                     EEYRRLIDGYMLRHKVRPGMTGLAQVHGCRGETETVDKMAKRVEWDLRYIREWSLWMD
                     IKILLQTFWVVLRGQNAY"
     gene            10794..12038
                     /locus_tag="PLD05_06980"
     CDS             10794..12038
                     /locus_tag="PLD05_06980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOP77216.1"
                     /translation="MSDAISSLACFPSFQTDAGNLFTVSPIADPVVVPICPEEPRTTT
                     ARVIHVINGEHYSGAERVQDGLALVLPQYGFDVQFVCLKPGRFAKCRRAQGAPVENLP
                     MRSRWDVAVASQIAALAEKTSAVLLHAHTPRSAMITALAARKCRLPWLYHAHSPTLRD
                     SGNWVKNFAVAMMEWWAVRQADHIIAVADSLKRYWQRWGISADRVSVVHNGVPSWPTL
                     PKRPHPGGTWTIGTVALFRPRKGIEILLRSLHQLRREKIPVRLLAVGPFETRKYEYHV
                     KSLAARLGLEDVVSWVGFTPHVDTLLAKMDLFVLPSLFGEGLPMVILEAMALGVPVVA
                     SRVEGVPEVIRDGQDGLCVTPGSVLALTHALRTVISGQVSWQSLREQAYRRQRQCFSL
                     ESMGRATADVYRHILRHWTTRS"
     gene            complement(12216..13127)
                     /locus_tag="PLD05_06985"
     CDS             complement(12216..13127)
                     /locus_tag="PLD05_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOP77217.1"
                     /translation="MSALILSTWHFGRIANVTGWPILENGGSALDAVERACAAVEEDP
                     EVTSVGYGGFPDASGEVTLDALIMVAPSRCGAVACLRGFIPAISVARRVMEKTSHVLL
                     VGPKAEEFAAAEGFTRRDLLTDRARRAWEEWKGRQNRQGQTAAEASVASTTADSRSNH
                     DTIGVLAQDAAGNLAGGCSTSGLAFKLPGRVGDSPIIGHGLYVDPDVGAAVGTGHGEL
                     IMRVCGTFLIVEEMRQGKLPKDAIRTALQRIADVCHPLPKQQAAFIALRKDGLWAAGA
                     LRPAFQVAIRSPTEDDLLAPQIVFWPE"
     gene            13479..14177
                     /locus_tag="PLD05_06990"
     CDS             13479..14177
                     /locus_tag="PLD05_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOP77218.1"
                     /translation="MMNPFWKNDEQLFAVFRQELSTAIVGEVLDQMGLNAQFLPGCIR
                     PLDPRMVVVGRAMPVLFADCFETFSPSGLNPVFAKPFGLMLEALDDLRPMEVFVATGG
                     RSELAMWSELMTVRAVRLGAAGALVDGCVRDTNALSRFSFPIFSRGAFAQDQRTRGKV
                     VDFRVAVQIGNVRVRPGDIIFGDHEGVCILPQGSEGDVVEAALQRLQKRAEARRELES
                     GSTAVEVFAKHNMI"
     gene            14294..16402
                     /locus_tag="PLD05_06995"
     CDS             14294..16402
                     /locus_tag="PLD05_06995"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOP77219.1"
                     /translation="MIVTEERDRIRGKALKAGGTIMLTVLFVLLGTVTVSEPANEWVV
                     LPGHSQLADPQSMMTTYLLGKIAEAEKRWREAYEKRTNLEEIKAHQAFLKEEFLRRIG
                     GLPERTPLNSQIVGRLERRGYRVEKVIFESQPKHFVTAALFLPDPNKFKPPYAAVLVP
                     CGHSNEAKAYRNYAGFGALCAFNGLAALVFDPIDQGERHQWRDPDGKFTLWGTAGHNM
                     IGVGSILLGRNTARYEIWDGMRAIDYLQSRPDIDPDKIGCSGISGGGTQTAYLMALDP
                     RIKAAAPGCYICSLYGRLIKTNGPQDAEQNIYGQLALGMDHADYCMMRAPAPTLLLTA
                     TRDYFDIEDAWMSFRMAKRLYGRLGYPERMELAETDEKHGFSIQLREASVRWMLRWLA
                     GRDEAVFEPSDLDVPTPAELQCTPQGEVMLLEGARSVYDLNRDYAKELAVRRQKLWQS
                     TPQEELINKVAQIVGLRRLAELPQPEVVACGTVRTEVCDVEKLIFRPEPGIELPALCF
                     KPRDRKPSAGVIYIHEDGKAADAGPGGPIEKLVRDGKLVLAVDLRGLGETQRTGQTYF
                     RPQYHGPDGQDFYLAYHLEKSYVGMRAEDLLVVARWVKQERLPADVPLEVVSVGYLNS
                     VALHAAVRELGLFSHLTLVRPMMSWHNVVELGFSKNRLVNTVHGALEVYDLPDLVRVL
                     GDRVTIIDPLDAMGEPWKES"
     gene            16636..17208
                     /locus_tag="PLD05_07000"
     CDS             16636..17208
                     /locus_tag="PLD05_07000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOP77220.1"
                     /translation="MTWTYFKRYRMEIALQQRELVEPPAPPGYSFLPWREDLLDIHAR
                     VKHLSFRGEIDAQIFPCFLDENSCRRLMLAIVRKPGFLPQATWLAVFSYQVEGKIYQE
                     YCGTIQGIYDAETRMGSIQNVGVIPGHRDRGIGQGLLYRCLRGFQQAGADRVSLEVTA
                     ENEGAIRLYRRVGFEIMRTVYKVAELPSAR"
     gene            17277..18200
                     /locus_tag="PLD05_07005"
     CDS             17277..18200
                     /locus_tag="PLD05_07005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP77221.1"
                     /translation="MRYTRRTFLAEATATGTGLVLGISAVSSGPQGVAAQASRDPTAI
                     VPLGKHLKASRIGIGFGMRGYNRQSNLSRRGLDHAERVVRYAYDVGIRFFDNADLYGS
                     HEYVAKALRGKPRDSYVLSTKVWFHPTGLPEKERPDADVAVKRFLKELETDYIDLVQI
                     HCMMNGEWTQTMRRQMDLLEELKEKGLIKAHGVSCHAVSALEAAAQSDWVDVVHARIN
                     HLGTKMDDKPEVVVPVLKRIHDAGKGVVGMKIIGEGAFRNDPELRDRSIEFALRSGCV
                     DVMIVGFEIPEEIDDFKQRVAAALDRLAEVA"
     gene            complement(18256..19539)
                     /gene="fabF"
                     /locus_tag="PLD05_07010"
     CDS             complement(18256..19539)
                     /gene="fabF"
                     /locus_tag="PLD05_07010"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOP77222.1"
                     /translation="MRRRVVVTGMGMVTPLGADLETVWRRLLNGESGIDYITLFDASH
                     FPTKIAAEVKNWDLSDVGENPEDWKFQGRHTHFAIGAAKKAWQDSGLEESKIDPTRFG
                     IYTGSGEGQQDFDRFTQMMVAALVDGELDLVKFTKKGLEILHPIVELEQEPNMPAGHL
                     AALFNAQGPNINCLTACAASSQAIGEAVEIIRRGEADIMLSGGTHTMIHPFGVTGFCL
                     LTALSTQNDPPQKASRPFDRNRDGFVLGEGAGMLILEELEHAKRRGAKIYGEIVGYGT
                     TADAYRITDTHPEGRGAISCMQMALRDAGLNPEDIDYINAHGTSTVVNDKVETLAIKK
                     VFGDYAYKLPVSSTKSMMGHLIAAAGATELIICLLAIRDRVLPPTINYEVPDPECDLD
                     YVPNVAREAKCDVALSNSFGFGGQNISLIVKRFTG"
     gene            complement(19682..20197)
                     /locus_tag="PLD05_07015"
     CDS             complement(19682..20197)
                     /locus_tag="PLD05_07015"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOP77223.1"
                     /translation="MRWYWIDRFIEFESGRYAKAVKNVSLAEEHLHDHFPGYPIMPPS
                     LIIEGVAQTAGLLVGEYNQFKMKVVLAKVPRAVFYHEAKPGDTLIYTATIDAIHPHGA
                     AVHATSHLGDKLQAELELVFAHLGDEFAGRQLFRPGTLTHMMRILRAYEVGRAADGSP
                     LVEPPDDRDAP"
     gene            complement(20292..20687)
                     /locus_tag="PLD05_07020"
     CDS             complement(20292..20687)
                     /locus_tag="PLD05_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOP77224.1"
                     /translation="MLTKEEIYQKVREVLMDALAVDEEEVRPDATLVGDLGAESIDFL
                     DIVFKLEKAFNIKIPRGELFPEDILTNPQFVKDGKFTEAGLEELRKRMPFAELDQFAR
                     NPLVQDFANLLTVADMCRFVELKLQQQGG"
     gene            complement(20811..21569)
                     /locus_tag="PLD05_07025"
     CDS             complement(20811..21569)
                     /locus_tag="PLD05_07025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOP77225.1"
                     /translation="MTQRFAGKVALVTGGSRGIGRACVAELAKGGAEVWFIYQSNQLA
                     AAGLAGELADQGATVHPEQADVRDFERIQQLAERIVNQCGKIDILVNSAGIVRDGLLG
                     TMTAEQWNEVLAVNLTGTFNCCRAVAQFMLSQRSGSIVNLSSTAAEFSSRGQVNYAAS
                     KGGINGFTRALAKELAPRNVRVNAVAPGMIDTDMSEAVRGLAGNKIKEIIPMRRIGTP
                     EEVAKLVAFLASDDASYITGQVIRVDGGLSLGGY"
     gene            complement(21715..22281)
                     /locus_tag="PLD05_07030"
     CDS             complement(21715..22281)
                     /locus_tag="PLD05_07030"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOP77226.1"
                     /translation="MRFSLIDRIVELQPGRALSAVKALSLAEEYLADHFPDFPVLPGV
                     LILEAITQAAAWLIRVSEDFQHSIVILKEAKRVRFGQFIEPGEILHVKVEVVSQDERF
                     TELKAEGRLNGRHSVNARIVMERYNLADRDPTCRSLDESIKRDLKKLFQIIAPDFQNL
                     VGTAGVPQRQETQSVVSQGTTVQITPAE"
     assembly_gap    22467..22566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22717..>24126)
                     /locus_tag="PLD05_07035"
     CDS             complement(22717..>24126)
                     /locus_tag="PLD05_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOP77227.1"
                     /translation="LSMATPINDWIDEPGVYRPLLRLAWPVLGEQALAMAVGFSDTIL
                     TGHYLGESHLAAVNLMAYTLWLLYGLFSVVAIGTSALVARYVGARRFTMADRFLHQSL
                     ILGLLISVPGAIAALTFGPDLVALLKLRGESAAAAVRYWNIIVPMTPCIMVIAVGIAA
                     LRGAGAMVHGLCIMAIVNIVNIAVSWACCLGWGPLPQLGWDGIALGTTCGYLVGAIGT
                     LTILGSGMIGLRLRWRMFRPEGHLIYRLLRVGIPGGLDSFALILCQLWFVSIVNVLGD
                     RAAAAHGVAIRIESLAFLPGAAFQVAAATMAGQYLGAKQPGKATRSVLVACLLGGLVM
                     GLGGVVFYIGAFPLASLMLGSHHHDVAYLAAPLLRTIAFGIPPLALVMILTGALRGAG
                     DTSWPLVFTLVGFLGVRIPVAYWLALSSVPIPWVNDPLPGLGLGVLGAWYAMVTDLYV
                     RAILTAWRFWQGGWKEVRV"
BASE COUNT         4958 a         6800 c         7169 g         5099 t
ORIGIN      
        1 cgcgggttca aaaaggcgtg gatttcatcg gagaaggcca acagccccac ggagtcgcca
       61 tgcttcaggg cgacatacgc cagtagcagc acggcattcc aggtgtggtc gagcatactc
      121 aaaccggccc attcgttggt catcatccga ccacaatcca ccatgaagac aacgcgctga
      181 ctttggctct gctggaagtc cttgacggtg agctttctcc gccgggcggt ggcgcgccag
      241 tcaatccagc gatactcatc atcgatggtg taatcccgca ggcgctcgaa ctcgtcctcg
      301 tgcccggcac ggcgaacacg ccgcacgcca ataagactga gccgattcac ccgggcaagc
      361 atggcgtatt cggaaagctg cttgatatca ggatagacat ggaggaccgt ctcgcaggga
      421 taggtcatct gccggaacca taacccgtaa cggctgttgc cgcggatata gacggccgac
      481 atggaaaagg ccccgcgtcg ctgcggacga agttcgtaat cgacaaccgt ccgtaagccg
      541 ggtctgagcg cgacgtgaat cagcggcggc gtcggctgca agatgcggtc ggcaccatcg
      601 cggatgaccg ccttcaccat ccaaggggcc agcgatgtga tggtcaggga cacaggatgg
      661 cgttttttca aggacgccac cttggcatgg ttgcgttcgc accgcaacca attttttctg
      721 ggaactgtga aaaggtccct gatgataatc acaagatagg caacatcgac aataagcacg
      781 gcgacccagg cgctttccaa cacaacaagc cccaatgtgg ccgtcacagg aatgaggccc
      841 aacagaacgg ctcgcagaga aggataaacc ccccacgccg ccgcggtggc taggagcgcg
      901 acgaatggca gggagaatcc gagaaacatc aggattgggg tcacagccgt gggacctcta
      961 cggagcggat gacctcgtgt aatacgtcgt ccacccgata gccttcgacc tcggcctccg
     1021 gtgccaagat gacgcgatgg cggagggccg gtagggccaa atcgaccaca tcgtcaggca
     1081 ccgcatagtc ccgaccacgg aaggcagcca gcgtccgtgc cgcttgcatc agagctaatc
     1141 ctgcccgcgg cgaagcccca acgaggattt gcggccagtt tcgcgttttc cgcaccaaag
     1201 cattgatgta attgaccagc ttcgggtcaa cgcgaacttc tcctgccaat cgcgtgacac
     1261 tcatgatttc ttcgggattc gtgacgacgc tcagttcctg caagcgctgg tcgatgtcct
     1321 cgcgcacact gtgcagaatc aggatcctgg cctcttcttc ctcccgcgga taatccataa
     1381 tcagtttgaa catgaagcgg tccatctggg cctcgggaag attgtacgtc ccctccgatt
     1441 caacaggatt ttgcgtcgcc aggaccataa acggtcgatc gagcttatgc cgtgtccgct
     1501 ctacggtgac actcagttcc tgcatgacct cgaggagggc agcgtgcgtc ttagccggcg
     1561 agcgattgat ttcatccgcc aggaggatat tcgtgaacac gggcccaggg cgaaagatga
     1621 actcttgggt tttcacatca aagatggggg aacccgtgat gtccgacggc atgaggtccg
     1681 cggtgaattg gattcgattg aacttacagc cgaggacccg tcccaacgtc cgcacaaaga
     1741 gcgtctttcc tagcccaggg acactttcaa ttaagacatg gccacccgaa aacagcgccg
     1801 tcaacgtccc cagaacaagc tcatcctgac cgacaaagac ctttcgtacc tctcgctcaa
     1861 tacgctcgta cagctcccgc acggggagca tcctttgaga aaggctacca gctgctagat
     1921 cgttgacagg cggcggactc gctgttacga cgacagcggc tgatggtccg gcaacggcct
     1981 cggccacgat aggcttctca acgactggat cacctgcggt cctgagccat tcggcggcct
     2041 tgacccaatt gctcatcccc tccgtccaaa gccagtcctc ggcagcgatc tcaccggtcc
     2101 ccaagcgacg ccgcagctca gcctcctcaa tcggtccaaa gcgattcggc cccttcgcat
     2161 agtaccaaag cctcatcaga gggactcctc acatttgaaa aatcggtaac cgttcccagc
     2221 gaacggggaa tctttctgag ctacacccgc aactccaaga tcatccttgg caacctcggg
     2281 agggtcgccg tcctcggtga ccctgcgcgg gcacggcagc tcggacggca ggcacgccat
     2341 ccctcccatc gccatatttt ctctacaata gaaggggttg tcacgcagac aaggataaca
     2401 attcccaagg gtcatcctcc cctatgcccc aaccttcggc aacctcggga gggtcatcgt
     2461 cctcggtgac cgcaattcgg gacggcgagg acgccgtccc tcccatcccg tgcggacaac
     2521 attcgcgtcg tttggaatcc tcgaaacagg acgataccga tgtggcgatc gatccttcga
     2581 caatgggcgc aacaaaccgc ggcggaggcc tttcaagctc gcgtggagga aatggcccac
     2641 gagaccgcgc agccggccgc tgatagcccc ccacggccca tcgacgtcgg tatcgtcttt
     2701 gctttagcat ccgagagtgg aggtctggag gaccgcctgg aagaaaagat ctggatccgt
     2761 ggtgaagccg gccaggtggt tattggccgt gctgggcaaa agaacgtggc catcattgtc
     2821 accggtcctg gggcgaagtc ggcccacgtt ggaacggagg ccttgattct cggtcatcat
     2881 ccccgctgga ttgtggccgc tggtttcgcc ggctctctca acgaacacgt tcgcccaatg
     2941 gagctaatca ttcccaaccg aatcaccgac ctgaccggcc gggccttgca ggtcggtctg
     3001 acgcttgacg cggaccaaac gaagggcatc catgttggtc cgctgctttc cgtgccagag
     3061 cttgtccgtc acccgctcga gcgccgcaag ctcgcggaag agcaccacgc ggtggctgtc
     3121 gatcttgaga gttttgccgt ggcggaggtc tgccgacgct accaggtccc catgtgggtg
     3181 gtgaagatcg tcacggacgg cgtcgatgat caacttccac cggaggtcag ctacttgatg
     3241 aagccccgca agtggactga aaagctcggg gctgtcgctg gggctttgtg gaaccgaccg
     3301 ggcagcatta gggacctttg gcaactcaac gaattggcac tccgcgccag cgaccgactc
     3361 gcggagttcc tcctttcaat gattcgctcc ctgccatgat gtgaaatcga tgccttcagt
     3421 tcagatctcc caaattacca aggattgggc aatggcttcg ctgtcttctt accgaggttt
     3481 cccggagctc ccaaatcact taaaaaacct atcctcctca gccgatatct cttgaaagca
     3541 gatggcggtc tgaggtttcc agcgggcggg tgtaaccaac gcgccaaatc ataaaggata
     3601 tgtaagccag ctgactatta taggtaccag ggcgccgggt gttagttgcg cggtgcgttg
     3661 gcacgcaagt cgcgcgatga cggccattta caataaagta gtggctgggg gtaacctgtt
     3721 gttgtgctgt tgacagtgag ttagcccttt gtcgtttgtg ttgatgattt agaggagttt
     3781 tcgcgtccgc tgttagtggt gtcaacaact tggtcggcta ttgacacggg gtgttaatgg
     3841 ctgcggatca actgcggcgg gacgggtgtg tctcgtccgc tgcggagcat atggtctgcg
     3901 gttcgccggg gatgagccgt tcttcaatca tgaagatggc ctgctgcatg ctggggtcgg
     3961 accggctaag ggcctcgatt ttacggcagc catgcccaac agtggtgtga tctcttcccc
     4021 caaagtaatg tccaatctgc tggaggctgg cacccgtcaa gcgttgggcc aggtacatgg
     4081 ccacgttgcg ggcaaggacc tctgcacggg agcgccccgg cccacggaga tctttcaggc
     4141 gaatcccgaa ataccgcgca gtagccttgg ctacccggtc gatagaggtt gccggacggg
     4201 gcgagtatcg tcgcaggaat gcccgcgcgg caggatagtc cactggtccg cgaccggcgg
     4261 cctgtcgcag ttttaaggca aatccgagga gctggcgggg cgagccggcg agctcttccg
     4321 caaagagttg gagcggagcc gcggtcacgg tgacgccctt ctggcgaaac gccatctgga
     4381 gaatcgccct gcgggcgtcc tcgccggggg gctgaaggcg agctgttaat cccccctgga
     4441 ggatggaccc aatgcggggg ttgagattct cgcagtgcca aggcgggata cgacaggcga
     4501 cggccacgca tagtgtgcca cgttcagccg cccgcaggag ggcgacaaac tcctcttgcg
     4561 ccgccgggta atcgccgagg aattggagat cgtcgatgac gatgaagtcc gcctcttccc
     4621 aatggcggcg gaattgacgg gctttgcggg aaacgagggc ctcggcgaag gcctgcccga
     4681 actcggcggc tgttgatgaa acaacggagc agtgtggcca gatttccttc caaaggccgc
     4741 aaagaaggcc caacaggtgc gttttacccg tccccgtcgg accgaagagt gtgaagcaac
     4801 tgggtgggtg gggctcaatc atcctttgga cagcggcaag ggctacccag ttctcttcgc
     4861 ccgcaataaa cgatcccggc tcgacacggc cggcggaccg gtcgagacca aggccgggca
     4921 gaaaaacaac tccaggcacg agtatctggg cggctccagt atccacggcg cgacagccct
     4981 tttgggtgag gtgaatcaac catccttcca cccagtgtaa ttggatttat catacctgct
     5041 tctccattgt ttgacgagcg aagatgggag gttttcctgg gaagacgcag cgggggtgga
     5101 ctctacagcc attggtgtgc catttgcttg gtttgacttt cagcccaata ggcctgccat
     5161 tttgctccgt agctcccgct acggcaaggc caccctatgg gttttgctga gatactgagc
     5221 aatcacggaa gcgaaagcgc ctcgttgaga ccgtgaatgt gtgcgcgtcg cctacgggga
     5281 agctttgttc aatccatagg ttttaatgtg ggtggtttgt attcctgggt ggttggcgta
     5341 gaatggacgg ggtgaaaaag accgcgcagg acctcctcag ggtctcaggt gtccatgccg
     5401 tgaacgctcg actttcggat cgaatccgcc cgccggctgt ggcgggaacc ttttatcccg
     5461 cccaacggga gcgtctcaga gaaatgcttg accagttcct ggccgaggca ccggtcattt
     5521 gcggacccga ccggacgatc cgcgctatca ttgcacccca cgcgggatat cgctattccg
     5581 gtccggtggc cggtgtggcc tataagcagc ttgctggccg ggattataac acggtggtct
     5641 tgctcggtcc gtcgcacttt gcccattttg aaggcggtgc cctgccagac gtggacgcct
     5701 ttgcgactcc gcttggcgaa gtggcgatcg caaacaaggc caggatcttg gcgcagaagg
     5761 aaccgtatgc ctttgatcct ccggccgtgg ttcaggcccc gcggggctgg acgaccacgg
     5821 ggacctcagc ctgcacacca catacatttg agcactcgct tgaggtgcag cttccctttc
     5881 tgcaagtcat cttgaaagag tttgagatcg tcccgatcgt tttcggtcgg gtgaatgtca
     5941 accaggctgc cgaggccctg gcgagtattc tcgacgaccg gacgctgatc gtcgtgagtt
     6001 cagatctctc gcattaccag ccttaccgtc gggcgtgtag tgttgaccag cggacactcc
     6061 aagcaatcat ccaaatggat ttcgattacc tcgagggtct ccgttacgag ccagagttgt
     6121 gtccttgtgg ccatacgggc attcttgtct tgcttcggtt ggcgagccgt ttccaatggg
     6181 aacccattct gcttgattat cggaacagtg gcgacacatc gggtgatcgc tcggccgtgg
     6241 tgggttacgg agccgtcgct ttttgtgagg gacgcgcgcg ctctgtggcc aaggcaaatg
     6301 aagaagaagt gaaggccgaa gaaatcaacc cggtggcaag tggtgcggtg tcggccagtg
     6361 gagatacgga agaagctccg agcgaagctc gtttgccaca actgactgcc gaagaacgcg
     6421 aattcttgct gaatttggcc tggaagacga tccgccgcta cgtcatcgat cgacgcttac
     6481 cggaagtcga gccggagtcg gttccacctc gcttacggga gccatgggcc tgttttgtga
     6541 cactccggcg gcgcggggcc ctgcgaggtt gtattgggac cttgacggcc gagcggccac
     6601 tttacctagc ggtgatggaa aacgctcgca atgccgcccg tgatccgcgt ttccctcctt
     6661 tggaagattg ggagttgacc gatctgacgg tagaaatcag tgtcttatca gaaaaacgcc
     6721 cgttggacgc ccgttcgccc gaggaaatcc tccgaaaact ccgaccgggc attgacgggg
     6781 tgattttgga gaggtttggt gctcgggcaa cgttcctccc ccaagtgtgg gaagatcttc
     6841 ccgatccggt ggagttcctc cagcgcttat cccaaaaggc gggcctgcct gccgatgctt
     6901 ggcgccaacc cgcgacacga attttcacct acgaggtgga gaaatttggc gattaggatc
     6961 gcggcgggcg gctcttccgg tgttagcttt tctgattccg taaaatgtgc cgattttcgg
     7021 gagtttttcg aaaaggtaac cgataacagt ttgcgaagag aatgcgactc ggattcttcg
     7081 ggaatgctgc cgaaaggatg gtttttgtcc cacaggtcag cacaagctcc cgttttgtct
     7141 taggaatacc ggcgaggtac cgaccatgcg cttgtggatg cccggcgtgg ttgtgttaag
     7201 cgtaatcctc atcggcggat gttgcgcgcc atgtcatcgt ttgggtatgc atcattacgc
     7261 cgccccgggg gctgtttatg gcgaggagcc cggtgtcgtc gcgtaccatg ctgggccatt
     7321 ggcatggtta tggcgcctgc tcggcgttgg acggggctat ccctgcgacg attgtggtcc
     7381 ccgctactgg ggcgattggg gtggtgaccg tgccgggtgc gaaccgtgcg acgactatgg
     7441 gtcgtggaca ggcatgccgc ccgttgaata tcgtggtgcc gctgggacct actcgagttc
     7501 cggcgacaag cgaggcggct gtccggagtg tcagtcgtca agctcgtcgg gcagtactgt
     7561 cagccaaatt aacccgcgga cgggacgcac aagtgtagcc gttcagtcta cgaaagagtc
     7621 gagcactcgg tgataagccg ataggcaccg agggcggtgt gccgcgggcc aaccacgtca
     7681 tcagacggtt gcgagagagg ccgttaggag aggaggtgcg cttgtctgga taagcgagtg
     7741 aggctcaaga cgatccgggg gaatcgtatt ccagaaaggg gcgccgattt cttcgatggc
     7801 ccgccagcgg cgaaggaagg cggcgacaac gtccggatca aactgcgttc ccgatccacg
     7861 ttcgatggtc gcgcgtgcca cgtctggggg ccaggccctc ttgtacaccc ggggcgatgt
     7921 gagtgcgtcg aaaacgtcgg ccacgctcac aatgcgggcg gcaagcggaa tgtcattacc
     7981 ggccaaaccg tcggggtagc ctttcccgtc atagcgctcg tggtgggagc gcgcgatgtc
     8041 ggcagcaatt tccaggaaag accctgcctt ggatttgttg cacgctgcca tgagagcctc
     8101 gtatccgatg atcgtatgtc gctgcatgat gaccgattct tcgggagaga gtcggccggg
     8161 tttcagaaga ataacatcgg gaattccgac ctttccgatg tcgtgaagcg gggcagaacg
     8221 gagtacctcc tgaggaaagt tttcggattc tgagtcgccg tgtgggcggc tggcaatata
     8281 ttcttcggcg ataagtcgtg aataccaacg catccgctcc aggtgttcac ccgtttcggg
     8341 atcgcgtgat tcagccaatt tagctagaga aaagattaac aggtcgcggg tctccaggat
     8401 ctcagcggtt cgctgcgaaa cgagcgcctc aagattgtcg gtgaacctgt ggagttgcat
     8461 gttcaagtcc cagacccgct cgaccatttt cttcaagcgg agatgaacgc cgatctttgc
     8521 aagtaattct tcgtggtcaa acggtttgac gatgtaatca tccgccccag ccgcgtaacc
     8581 ctcgagacgt tcctgcgtgg aacttttacc gctcacgaga ataacctggg tgaagggtcc
     8641 caacggccct gatttgattt gccggcaagt ctcgtaaccg tttagaccgg gcatcatgat
     8701 atcgagaaga acgacatctg gcgctgcctg cggcaagatt cgtaaagcct gctcaccatt
     8761 tggtaccgtc agaacgtaat actcgtctga aagaaaagct ccaagagaat ctctctgttg
     8821 acttcgcagt catcaacaac caggatggtt ggcttttgtg agtccatgtt cttcccccat
     8881 tgaggattca cctggccacg atgagttgtg gcagttcctg gtcggaccgt tctcctaaag
     8941 tggcggctgc cttcggccta gcacgatgaa tctgtgcata aaaagcatag gttggcggca
     9001 ttaatcgcgc gcgtacccgt aatggtgacc agaccaggaa aagggcaaga ccgacagcac
     9061 cggcccatca cagccgatag aatcgctaca atcggcacgc acagccatct gcggacggga
     9121 aaaaccggca attatgtgct gaacagtcga cgagcttctg ccgctgcgga aggcttctca
     9181 tccggtgccg ccgcacctaa accgatcgga aaacctgtca gaaattctgc aacctggaat
     9241 tgagctatat tcattgggat tgtcaatcgg gtcttttagc ccggcgaaga acattccgaa
     9301 gtaagtgttt gcttgcgata cacgtgacat taacaatcga aggcggcggc gatgagtggt
     9361 tcgcaacgtg tcattcatca atactggtcg gtgcgtgagt tattctatca cgtgctggac
     9421 ggggccgtca tcgtcggggc cctttggtgc gctcggctaa ccctcggcgg cttccgggaa
     9481 gaggacctcc ttgctgcctt gctggcagtc atcgtgtttc atgtggtggc cgagatcacc
     9541 gggctgtacc gcaattggcg gggcgtttcc ttggaaaggg agttgctttg tgccgccatc
     9601 acatggttct tcacgttgcc ggcgctcgtc actttgggat actttgtcga tcatgggcgt
     9661 atcgtttccc gcgagaccgt gctcgtttgg gccggcatgg gctttctgct gttactcgta
     9721 tcgcgaagcg ccgtccggtg ggtcctcaac aggcttcggg ccagcggata caacacccat
     9781 tattatgcgg ttgtcggcat caacgagttg gcctttcgcc tggtggaatc cctggagaac
     9841 tcacccgatt tgggcttgaa gctctacgga ttctatgacg accgaccgga gtcgcggacg
     9901 gggcctgtcc ccgaggaaat cggccagaaa ctgggcaaca tcgaggagct cgttgagcac
     9961 gcccggcagc gacgcgttga tatgatctac attacttttc ccatgcgggc ggaagatcgc
    10021 atccggaaga ttctggccca actgtccgac accacggcgt ccgtctatat cgtgcccgat
    10081 ttctttgtgt ttcaattgct ccattctcgc tggaccaaca ttggggggct gcccgtcgtc
    10141 agcatctacg aaaacccatt ctacggcatt gacggctttg tgaagcgggc tatggacgtg
    10201 gtcttggcga gcattttcct cgtgttgctg tcgcccctca tggtggcgat cgccattgcc
    10261 atcaagttaa catcgcctgg gcctgtattc ttccgtcaac ggcgttatgg gctggatggg
    10321 cgagaaatcc gtgtatggaa gttccgcacc atgacggtgt gcgaagacgg acagcaagtg
    10381 acgcaagccc gaaggggtga cccacgggtc acgagaatcg gtgcctttct ccgtcgtacc
    10441 tcgctcgatg agctgccgca attattcaac gtgctgaagg gagatatgtc gcttgtggga
    10501 ccgagacctc acgccacggc gcacaacgag gagtaccgcc gactgatcga cgggtacatg
    10561 ctgcggcata aagtcaggcc cggcatgaca ggtctcgccc aggtccacgg gtgccggggg
    10621 gagaccgaaa ccgtggacaa aatggcgaag cgcgtggaat gggacctccg ctacattcgc
    10681 gaatggtctt tgtggatgga tatcaagatt ctcctgcaaa cgttttgggt cgtcttgaga
    10741 ggtcagaatg cttactgatc ggttcgtcgt tttcagaaaa gggccacgcc gcaatgagtg
    10801 acgcgatttc gtccctcgcc tgttttccat cgtttcagac cgatgccggt aacctcttca
    10861 cggtttcgcc gatagccgac ccggtggtgg tacccatttg tcccgaagaa ccgcggacga
    10921 cgacggcccg cgtgatccat gtcatcaatg gggagcacta ttccggggcc gagcgggttc
    10981 aagatggttt ggccctcgtc ctgccacagt acggcttcga cgtccagttc gtctgcctga
    11041 agccgggccg atttgccaag tgccgccggg cacaaggggc tccggtggag aaccttccca
    11101 tgcgttcgcg ctgggatgtt gccgttgctt cgcagattgc cgcgttggca gagaagacct
    11161 cggcggtgct tctccatgcc catacgccgc ggtccgcgat gattacggcg ctcgctgcgc
    11221 gaaagtgtcg cctcccttgg ctctaccacg ctcatagtcc cacgcttcgg gattcaggca
    11281 actgggttaa gaactttgcg gttgcgatga tggaatggtg ggcggtccgc caagcagacc
    11341 acatcattgc ggtggcggac agcctcaagc gatattggca gaggtggggt atctcggcgg
    11401 accgggtgag tgtcgtccac aatggtgtgc catcctggcc gacccttccc aaacggcccc
    11461 atccgggcgg aacgtggaca atcggaacgg tggccctctt ccgaccgcgg aaaggaatcg
    11521 agatcctttt gcgttcgctg caccagcttc gccgtgagaa gatcccggtg cgcctcttgg
    11581 ccgttgggcc gttcgaaact agaaaatacg agtatcacgt gaaaagcctg gcggcccgct
    11641 tgggcctgga agatgtggtc tcctgggtgg gcttcacgcc gcatgtggat accttgctgg
    11701 cgaagatgga cctgtttgtc cttcccagcc tctttggcga gggcctcccg atggtcattc
    11761 tcgaggcgat ggccctgggc gtccctgtcg tggcaagccg ggtggagggt gttccggagg
    11821 tcatccgcga tggccaagac gggctttgtg tgacgcctgg ttccgtgttg gccttgacgc
    11881 acgccttgcg aacggtcatc tctgggcaag tcagttggca atcgttgcgc gaacaggcct
    11941 atcggcggca acgacaatgc ttttctttgg agagcatggg acgcgctacg gcggacgttt
    12001 atcgccacat cttgcggcat tggacaaccc gctcataaga gcctatccca aaatttcctc
    12061 tccctttggg agagggttag ggtgagggca agcgatcgtc ggagtcgatc gagaaaacca
    12121 gcagcgattc gagccaccct cacccccggc ctctctcccg gcgaacgggc gaggggaggt
    12181 tttgggatac gctctaagtc ttcgagaaac gccgttcact ccggccaaaa cacgatctgc
    12241 ggggccaaca gatcgtcctc cgtgggactg cgaatggcga cctgaaacgc cggccgcagc
    12301 gcaccggcgg cccaaaggcc atcttttcgc aatgcgataa aagccgcctg ctgcttgggc
    12361 aaggggtgac aaacgtcggc aattcgctga agggcagtcc tgatggcgtc cttgggcagc
    12421 tttccttggc gcatttcctc cacgataagg aatgtcccgc agacccgcat gatcagttcg
    12481 ccgtgaccgg ttcccacggc ggcgccgacg tccggatcca cataaagccc gtgcccgatg
    12541 atgggcgagt cgcccacccg acccggcagt ttgaaggcca agccactggt ggagcacccc
    12601 ccggccaggt tccccgccgc gtcctgtgcc aagacaccga tggtgtcgtg attgctacgt
    12661 gaatctgcgg tggttgaggc aacgcttgcc tcggcggcgg tttgtccctg gcgattctgt
    12721 cgccccttcc attcctccca ggcacgcctt gcccgatcgg tcagcaaatc tcgacgcgtg
    12781 aacccctccg cggcggcgaa ttcttccgcc ttagggccga cgaggaggac gtgcgaggtc
    12841 ttttccatga cacgtcgggc cacggagatc gccggaatga acccccgcag acaggcgacc
    12901 gccccacagc gagaaggggc caccatgatg agagcgtcta aggtcacctc gcccgacgca
    12961 tccgggaagc cgccgtaacc gacgctggtc acttctgggt cttcctcaac cgccgcgcag
    13021 gccctttcta cggcatccag ggcagagcca ccgttttcca agatgggcca gcccgtcacg
    13081 ttggcgatcc gaccaaaatg ccatgtactg agaatcaagg ccgacattgt cacctcgcac
    13141 agaaggatgg tttcagatga ttatgctatt ttgacgcaga acgcggtagt gaccaacact
    13201 acgtccagac gccagcaggg ggcttttcgc gatcattttg gcggcagtgt ccaacgacga
    13261 ttcgtagcga ttctgctggg ccatgcgctg cggtagccgt ggcgcacttg tgcgggccga
    13321 aggttggggc ttttgccgga ttacgggctt ggggaaaatt gacaaactga atattttgcg
    13381 caaacttatg ttagcgacta ccctctcact cttgagcgtt ggtcgcaagt ggcgccgcag
    13441 ttgcggggtg tgtgccagga tcttttctgg agacaaccat gatgaatcct ttctggaaaa
    13501 acgacgagca gctctttgcg gtgtttcggc aagagctttc cacggccatc gtgggcgagg
    13561 tgctcgacca gatgggcctc aacgcgcagt ttttgccggg ctgtattcgg ccgctcgatc
    13621 cgcggatggt ggtggtcggt cgggcgatgc cggtcctctt tgccgactgt tttgagacgt
    13681 tttcgcccag cggcttgaat cccgtttttg ctaaaccctt tggcttgatg ctggaggccc
    13741 tggatgattt gcgtcccatg gaggtgtttg tagcaaccgg tggccgaagc gaactggcca
    13801 tgtggagtga attgatgacg gtgcgggccg tccgactggg cgcggccggg gccttagtcg
    13861 atgggtgcgt ccgggatacg aatgccctca gccgtttttc cttccccatc ttttctcgtg
    13921 gtgcctttgc ccaggaccag cggacacgtg gaaaagtcgt tgactttcgc gtggccgtgc
    13981 aaatcggcaa tgtgcgggta cggccgggtg atatcatctt tggcgatcac gaaggggtgt
    14041 gtatcctccc gcaaggttcg gagggtgacg tggttgaggc ggcattgcag cgactacaaa
    14101 agcgagcgga ggcccgccgc gaactcgaat ctggttccac cgctgtggaa gttttcgcca
    14161 aacacaatat gatatagggc gaatgacgga ggcgtgatcc tgcgcaggag gcggtcgggc
    14221 attgcccgag atgatcgccg catccaaccc tctgtggagg ctggatcgtc ataccaaagg
    14281 attgtgtgtc cggatgattg tcacggaaga aagggatcgt attcgcggaa aggccttgaa
    14341 ggccgggggg acgatcatgc tcactgtact gtttgtcctg ctgggtaccg tcacggttag
    14401 tgaacctgcc aacgagtggg tggttttgcc gggtcattcg cagcttgccg acccccaatc
    14461 catgatgacg acgtatctgc tggggaaaat tgccgaggca gaaaagcggt ggcgggaggc
    14521 ctacgaaaaa cgcacaaatc tggaagagat caaagcgcat caggccttct taaaggagga
    14581 gttcctgcgg agaattggtg gcctgccgga gcgaacaccg ctcaattctc aaattgttgg
    14641 ccgacttgag cgccgcggtt accgagtgga aaaagtgatc ttcgaaagcc aaccgaagca
    14701 ttttgtgacg gcggcgctct ttttgccgga tcccaacaag ttcaaaccac cgtatgcagc
    14761 cgttttggtt ccgtgtgggc attccaacga agccaaggcc tatcgcaact acgcgggttt
    14821 cggggccttg tgcgcgttta atgggctggc agcattggtc tttgatccca tcgaccaggg
    14881 agaacgtcat cagtggcgcg atcctgatgg aaagttcacc ctatggggaa cagccggaca
    14941 taatatgatc ggcgtcggtt ccattctttt gggccgcaac accgcacgct acgaaatctg
    15001 ggatggcatg cgtgccatcg actatctgca atcgcggccc gatatcgatc cagacaagat
    15061 cggttgctcg ggaattagtg gcggcgggac gcagacggcc tacctcatgg cacttgatcc
    15121 ccgcatcaaa gcggctgccc cgggctgcta catttgcagt ctctatggtc gactgatcaa
    15181 gaccaacgga cctcaagacg cggagcagaa tatctacgga caattggccc tggggatgga
    15241 tcatgccgac tactgcatga tgcgggcacc ggccccgacc cttcttttga cggcgactag
    15301 agactacttc gacatcgaag atgcctggat gtccttccgc atggcgaagc ggctttatgg
    15361 tcgcctgggg tatccggaac gaatggaact tgccgagaca gacgagaagc acggcttcag
    15421 cattcaactg cgtgaggcct ctgtgcggtg gatgctccgt tggctggcgg ggcgagatga
    15481 ggcggtcttt gaaccttccg atctggatgt cccaacgccg gcggagctcc aatgcacgcc
    15541 acaaggcgag gtcatgctcc tagaaggggc ccgctcggtt tacgatctca accgtgatta
    15601 cgccaaagag ttggcggtgc gacgtcagaa actgtggcaa agcacgccgc aggaagaatt
    15661 gatcaacaag gttgcccaga ttgtgggctt gcggcgactg gccgagttac cccagccgga
    15721 ggtcgtggct tgtggaacag ttcgcaccga agtttgcgac gtggaaaagc tcatttttcg
    15781 tcctgaaccg gggattgaat tgcccgccct ctgtttcaag ccgcgcgatc gaaagccatc
    15841 cgcgggcgtc atctacatcc acgaggatgg caaggcagcc gacgccggtc cgggagggcc
    15901 catcgaaaag ctcgttcggg atggcaaact cgtcctggcg gtcgatcttc gtggattggg
    15961 cgagacgcag cggacgggcc aaacctattt ccgcccacaa tatcacggcc ccgatggcca
    16021 ggatttttat ctcgcttatc acctggaaaa gtcctatgtg ggcatgcggg cggaagatct
    16081 gctagtcgtg gcacgctggg ttaaacagga gcggttaccc gccgatgttc ccttggaagt
    16141 ggtgtcggtc ggctatttga atagcgtggc actgcatgcg gcggtaagag agctggggct
    16201 tttcagccat ctcacgctag tacgaccgat gatgtcttgg cacaatgtgg tcgagctggg
    16261 ctttagtaaa aatcgcttgg taaatacggt tcacggggca ctagaggttt acgatttgcc
    16321 cgatctggtt cgggttctcg gcgatcgggt gacgatcatc gatccactcg acgccatggg
    16381 tgaaccctgg aaggagtcgt gatccagccc gggacctatt agccgctggg cgcatggaga
    16441 gcgccgcggg acgcgtgatc cacaacggga tgccgcgggt gcctatttgc gtgatctagc
    16501 taaagaagga cgggccaagt cagtttttgg gacactgttg acccgggaag tgggggagcc
    16561 tagatttagg ttaaggcagt attccgaggc tttgtgggcc gtaccggctg cgttcataat
    16621 gtccccgcga acaggatgac ctggacgtat ttcaaacgtt atcgtatgga gatcgctctc
    16681 cagcagcgag agttggtgga gccgccggcc ccgcccggct attcgtttct gccttggcgc
    16741 gaagaccttc tcgacatcca tgcccgggtc aagcatttga gttttcgcgg ggagatcgac
    16801 gcgcaaattt ttccctgctt tcttgatgag aacagttgtc gtcggctgat gctggcgatt
    16861 gtccgcaaac cgggtttttt gccccaggcc acttggttgg ccgtcttttc gtaccaagtt
    16921 gaggggaaga tttaccagga gtattgcggc acgattcagg gaatctacga tgccgaaacg
    16981 aggatggggt cgattcaaaa tgtgggcgtg attcccggcc accgagaccg cggcattggg
    17041 caggggttgc tgtatcgctg cttgcggggg tttcagcagg cgggggctga ccgtgtttcg
    17101 ctggaggtca cggcggaaaa cgaaggtgcc atccgccttt atcgacgggt cggtttcgag
    17161 atcatgcgga ccgtctataa ggtggccgaa ctccccagcg ccaggtaaaa ttgtttcgtc
    17221 ttcatccgaa gggtgccgaa gcactgtgat gccatcttcg taaaaggagc tatatcatgc
    17281 gctacacccg acgcacgttt ttggccgaag cgacggcaac gggaactggt cttgtgctgg
    17341 gaatctcggc ggtatcgtcg gggccccaag gggtggcggc ccaggcttcg cgggatccga
    17401 cggccatcgt ccccctggga aaacacctca aggccagccg gatcggtatt ggctttggca
    17461 tgcggggata caatcggcag tcgaatctct cgcggcgagg gcttgaccat gccgaacggg
    17521 tcgttcgcta cgcctacgat gtgggtatcc gctttttcga caacgccgac ctgtacggca
    17581 gccacgaata cgtggcgaag gccctccgtg ggaagccgcg agacagctac gtcctgagca
    17641 cgaaggtctg gtttcatccc acgggcttgc ccgaaaaaga acgccccgat gccgatgtcg
    17701 cggtgaagcg ctttctcaaa gagctcgaaa ccgattacat cgacctagtg caaatccact
    17761 gcatgatgaa cggcgaatgg actcagacga tgcggcggca gatggacctc ttggaggaac
    17821 ttaaggaaaa gggtctgatc aaagcccacg gggtttcctg ccatgccgtt tcggccttgg
    17881 aggcggcggc tcagtccgat tgggtcgatg ttgtccatgc ccgcatcaat cacctgggca
    17941 ccaagatgga cgacaaaccc gaggtcgttg tgcccgttct gaaaagaatt cacgatgccg
    18001 gcaaaggtgt tgtggggatg aagattattg gagagggcgc ttttcgcaac gaccctgaat
    18061 tgcgggaccg atcgatcgaa ttcgcactcc gcagcggctg tgtcgatgtg atgatcgtgg
    18121 ggtttgaaat cccagaagaa attgacgatt tcaagcaacg agtggcggca gccctcgatc
    18181 gcttggcgga agtcgcctga tggattggat ctctgagcgc gatccagcgc cggggtgtca
    18241 atcattaacc cggactcagc ccgtgaatcg tttcacgatc aggctgatat tctggccccc
    18301 gaatccaaag ctgttgctta gggccacgtc gcacttggcc tcgcgggcaa cgttggggac
    18361 gtagtcgagg tcgcattccg gatcggggac ctcgtaattg atggtcggcg gtaggacgcg
    18421 atcccggatg gccaacaggc aaatgatcag ctcggtggcc cccgctgcgg caatgaggtg
    18481 ccccatcatg ctcttggtgc tggagaccgg gagcttgtag gcgtaatcgc caaagacttt
    18541 ctttatggcg agcgtctcga ccttatcgtt gaccaccgtg ctcgttccgt gggcgttgat
    18601 atagtcgatg tcctcgggat tgaggcccgc atcccgcagg gccatttgca tgcaactgat
    18661 agcaccgcgg ccttcggggt gggtatcggt gatgcggtag gcatcggcgg tcgtgccgta
    18721 gccgacgatc tcaccgtaaa tcttggcccc acggcgtttg gcgtgttcca gctcttcgag
    18781 gatgagcatt ccagcccctt caccaagaac gaatccgtcc cgattgcgat cgaaggggcg
    18841 cgaggccttt tgcgggggat cgttttgggt actcaaggcg gtgagcaggc aaaatcccgt
    18901 gaccccgaag gggtgaatca tggtatgcgt tccacccgaa agcatgatgt cggcctctcc
    18961 ccgacgaatg atttccaccg cctcgccgat ggcttggcta gatgccgcac aggccgtgag
    19021 gcaattaatg ttggggcctt gcgcattgaa cagggcggcc aagtgtcccg ccggcatatt
    19081 gggttcctgc tctagttcca cgatggggtg gaggatctcc aggccctttt ttgtgaactt
    19141 gacgaggtcc aattcgccgt caaccaaagc ggccaccatc atttgggtaa agcggtcgaa
    19201 atcctgctgg ccttcgccgc tacctgtgta aatgccaaac cgcgtgggat cgatcttcga
    19261 ttcctcaagc ccggaatctt gccaggcctt tttggcggcg ccgatggcga aatgggtatg
    19321 gcgtccctgg aacttccagt cctcagggtt ttccccgaca tccgaaaggt cccagttttt
    19381 cacttcggcg gcgatcttcg tgggaaaatg gctggcatcg aagagggtga tgtagtcgat
    19441 ccccgactca ccgttgagca gacgacgcca aaccgtctcc aggtcggcgc ccaacggtgt
    19501 gaccataccc attcccgtaa caacaacccg acgtctcatc tgatatcata ctccgtaagg
    19561 ataggacact tgagtgtctt ttgcctcgat cgcaaatgtg tggtggcatc cctacgatgg
    19621 ataggcaggc cgtcttggcc gagccgcatc gtctggccca tgcgattcac cccacagaat
    19681 gctatggggc atctcggtca tcgggcggtt ccacgagggg ggaaccgtcg gctgcccgcc
    19741 ccacttcgta cgcccgcaaa atccgcatca tatgggtgag tgtccctggt cggaacaact
    19801 gccgacctgc gaactcgtca cccaaatggg cgaacaccaa ttccagctct gcctgaagtt
    19861 tatctccgag gtggctggtg gcgtggacgg ccgccccatg ggggtggatc gcgtcaatgg
    19921 tggcggtgta gatcaacgtg tctcccggtt tggcctcgtg ataaaagacg gcccggggga
    19981 ctttggccaa gaccaccttc atcttgaatt gattgtattc ccccaccaaa agacccgccg
    20041 tttgggcgac gccttcgatg atcaaactgg ggggcatgat ggggtagcct ggaaaatggt
    20101 cgtggaggtg ttcctcggcc aaggagacgt ttttcacagc cttggcgtaa cgaccacttt
    20161 caaactcgat aaatcgatcg atccaatacc agcgcatacg ttttggcttt tcctatcagc
    20221 ggcaaagcaa gcgacccggc agcatcccgc ccagtcgccc gcagcctgga tgccccccgg
    20281 cgggtcgcca atcagccgcc ttgctgttgc agcttaagct cgacaaagcg gcacatatcg
    20341 gccaccgtga gcaggttcgc gaagtcctga accaacggat ttcgggcgaa ctgatcaagc
    20401 tccgcgaagg gcatacgctt gcgaagttcc tccagaccgg cctcggtgaa tttgccgtcc
    20461 ttcacaaatt gcggattggt caaaatgtcc tcagggaata actcaccccg cggaattttg
    20521 atgttgaagg ccttttccaa tttgaagacg atgtcaagga aatcgatgga ttctgcgccg
    20581 agatccccca caagagtggc atcagggcgt acttcttcct cgtccaccgc caaggcgtcc
    20641 atgaggactt cccgtacctt ttggtaaatc tcctcctttg tcagcatttg gagtctccct
    20701 ttgaccagtc cgaagtcgca aattggaaaa cgtttcccga taatcgtttc atttagccga
    20761 aatttgtcgt tttgcctagt ctaggtagtc ccctttctgg ggaagaattg ttaatacccc
    20821 cccaagctca gtccaccgtc cacacggatc acctggccgg tgatatacga cgcatcatcg
    20881 ctggcgagga aggccacgag tttggcgact tcctcgggcg tgccgatgcg ccgcataggg
    20941 ataatctcct tgattttgtt tcccgccaaa ccgcggacgg cttcgctcat atcggtgtcg
    21001 atcatgcccg gggccacggc attgacgcgg acgttccgcg gggccaactc cttagccaat
    21061 gcccgcgtga aaccattgat gccaccctta ctggcggcgt aattgacctg tcctcgtgaa
    21121 gaaaactcgg ccgcggtgct ggagaggttg acaatactcc cgctccgctg ggacaacata
    21181 aactgggcca ccgctcgaca gcagttgaac gtccccgtca ggttgaccgc caaaacctcg
    21241 ttccactgtt cggccgtcat agttcccagc aggccgtctc gaacgattcc cgccgagttg
    21301 acgagaatgt cgatctttcc gcattggttg acgatacgtt ctgccaattg ttgaatgcgt
    21361 tcaaagtctc gcacgtcggc ttgctctgga tggacggtcg ccccttggtc ggctaactcg
    21421 ccggccaaac cggctgccgc tagttggttg ctctggtaga tgaaccagac ctcagccccg
    21481 cccttggcca attctgcaac acatgcccga ccgatccccc ggcttccacc ggtgacaagg
    21541 gcgacttttc ctgcgaatcg ttgtgtcatg ttggcatatc ctatcggctt gatcccattt
    21601 tcaactggtg atttgttcag tcagtgcgaa gaacccctca ctctgtgtca atgcaagata
    21661 ccaactgtat cgcacatcat ttgcgggaca aacaggttgg gataatccaa agggtcactc
    21721 tgccggggtt atctggaccg ttgtgccttg agaaaccacg ctttgcgtct cttgccgctg
    21781 cgggacaccg gccgttccca cgaggttttg aaaatcgggt gcaatgatct ggaataattt
    21841 tttcaaatcc cgcttgatag attcgtccag cgaccgacac gtcgggtcac ggtcggcgag
    21901 gttgtaacgc tccatgacga ttcgggcatt aacgctgtgt cggccattga ggcggccttc
    21961 ggccttaagc tccgtgaacc gttcgtcttg gctgaccacc tcgaccttca cgtggagaat
    22021 ctcgcccggc tcaataaact gcccaaaacg gacccgtttg gcctccttta aaatgacaat
    22081 gctatgctga aagtcctcgc tcacacggat cagccaggcc gcggcctgcg tgatcgcctc
    22141 gaggatgagc acgccgggga ggacgggaaa atcgggaaag tggtcggcga gatactcttc
    22201 cgccagagag agggccttga cagcggaaag tgcccgcccc ggttgcaact cgacgatccg
    22261 gtcaatcagg gaaaaacgca taatacttca atgtcatcgc ttaaaggcag tatgcccggc
    22321 gacggaaagc caagctatgg ggaataaaga atttaccacc tatgttaatc cgttgagtat
    22381 aaaaagaaac cgtttccatc acaacacggc ttttcccgag gtttgggaaa aatcccccgt
    22441 aggggcaggc ccctgtgcct gccccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnntagg ggcaggcccc tgtgcctgcc ccaattaccc gccccgtgcc tgccctattc
    22621 cacatcccgt gcccgcccta attacctgcc ccgatggcca aaaaccaacg cgatcccggc
    22681 gtatagtgtt tgtctcgggt tttatgtaaa cgtggattag acgcgaactt ccttccagcc
    22741 gccttgccaa aatcgccacg cggtcaggat cgcgcggacg tagaggtccg tcaccatggc
    22801 gtaccatgcg ccgagaacgc cgagccccaa gcccggtaag gggtcgttaa cccaggggat
    22861 gggcacgctc gagagggcga gccaatatgc caccgggatg cggaccccga ggaagccgac
    22921 cagcgtaaag accaacggcc aacttgtgtc gcccgccccg cggagggccc ccgtgaggat
    22981 catcaccaag gccagcggcg ggataccaaa ggcaatggtt cgcaacaaag gagcggccag
    23041 ataggccacg tcgtggtggt gcgatcccaa catcaagctg gccagaggga aagccccgat
    23101 atagaagacg actcctccaa gccccatgac cagccctccc aacaaacagg ccaccagcac
    23161 gctccgtgtg gccttgcccg gttgtttcgc cccgagatac tgcccggcca tggtggcggc
    23221 ggcgacttga aaggccgcac ctggcaaaaa ggcgagcgat tcaatgcgga tggccactcc
    23281 gtgggcggcg gctgctcgat ctcctaagac attgacgatc gagacaaacc acagttgaca
    23341 gagaatcaag gcgaacgaat ccagtccacc aggtatgccc acccgcaaga gccggtagat
    23401 caaatggcct tccggacgaa acattcgcca tcgcagacgg aggccgatca ttccactgcc
    23461 aaggatcgtg agcgtgccga tggcccccac cagataaccg caggtcgtgc ccagcgcgat
    23521 gccatcccag cccaattggg gaagcgggcc ccagcctagg caacatgccc aacttacggc
    23581 gatgttaacg atgttgacga tggccatgat gcacaggcca tggaccatgg ccccggcccc
    23641 gcgcaacgcg gcgatcccca cagcgataac cataatgcaa ggggtcatgg ggacgatgat
    23701 gttccagtag cgaacggctg cggctgccga ctctccgcgg agtttaagaa gtgccaccaa
    23761 atccggccca aaggtgaggg ccgcgatggc ccctgggacg gaaatgagca atccgaggat
    23821 taacgactgg tgaagaaaac ggtcggccat ggtaaagcgg cgggcgccca catagcgagc
    23881 caccagggcg gatgtcccaa tcgccaccac gctgaagagt ccatacaaaa gccacaaggt
    23941 gtaagccatc aaattgacgg cggccaggtg ggattcccca agataatgcc ctgtcaaaat
    24001 cgtgtcggag aaacccacag ccatggccag ggcctgctcg ccaaggacag gccaggccag
    24061 gcgaagaagc gggcgataaa ccccgggttc gtcaatccaa tcgttgatag gggtagccat
    24121 actaag
//
LOCUS       DAOSUT010000057        23965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1378_length_23965_cov_9.345211, whole genome shotgun sequence.
ACCESSION   DAOSUT010000057 DAOSUT010000000
VERSION     DAOSUT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 23965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23965
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_23965_cov_9.345211"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3392)
                     /locus_tag="PLD05_07040"
     CDS             complement(<1..3392)
                     /locus_tag="PLD05_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOP77228.1"
                     /translation="MTQSTERVPFLTRIVDVFLRGDSAIMLTVISVLIGLGALWITPR
                     EEEPQIVVPMADIFVSAPGLSAQEVEQQITNRLERIVYQIDGVEYVYSMSRPETAIVT
                     VRFYVGQDREDSLVKLHAKVQSYIDEAPSSVKGWVIKPVEIDDVPIVNITLWSRDPEH
                     AHDYELRRIAEQLQNELKSIPQTNRVTIVGGRPRHIRVEVDPIKLAARSTSILQVAQA
                     LKVSNVNVRAGRYEQRNREFLVEAGTFVRDARELENLVVNVVGNRPVYLKDVAQVIDG
                     PAEPVSYTWMGFGAAAKHPEAPREHVTPEDLKSKPRAIPLQYFPAVTIAVAKQKGTNA
                     VWVAQAVEERIAQLAKTHLPSNVFYRITRDYGETANEKVNELVEALIVAVLTVIGLIW
                     LFVGWRAAVVIALAVPVCYSVTLFINYLAGYTINRVTLFALILALGLLVDDPITDVEN
                     IARYFAMRILPPRPAVLRAVQEVRPALVMSTLAIVASFAPMAFITGMMGPYMAPMALN
                     VPVTLMVSLIVSLVITPWMAMFALQGSKEHGDSTEQGYDLTKRPIYRMVRAVFHPILN
                     YRATAWGVVLAVAILFVASLLLPGLRVVPLKMLPYDNKNEFQVVIDMPEGTTLEQTDA
                     AARRIAAYLATLPEVRDFQIYAGLSSPMDFNGLVRHYFLRQGSHLADIRVNLLHKDFR
                     AQQSHEIILRIRAEIAKIAQEIDANIKLVEVPPGPPVISTITAEVYGPPDASYDDLIR
                     AAKFVEARMHREPGVVDVDISAEADQEKRLFVTDQPKAALSGVSKEMIAQTVQAALGG
                     YPATELHIPSEVNPLPVILRLKRSERSAEDDLAELYVPGSQGQLLQLGSLGKFVSTSP
                     EGKPLLEDKTIYHKNLRRVVYVFGEVAGRPPADAIIDMQVDLVPENEFPRKKPQPRPV
                     EARTWFHPGGKDFWTMPEGYQAVWTGEGEWKITLDVFRDLGIAFGVALVVIFILLMFE
                     TKSRVLPLIIMLAIPLTMIGIMPGFWILNLITNRPVGGFANPVFFTATAMIGMIGLAG
                     IVVRNSVVLIDFIHHSLLDGFDLKEAIVRSVAVRFRPIILTSGTTLLGNWVITLDPIF
                     SGLAWAIIFGIFASTTFTLVVIPTVYWLLYAKRGGAIAVPSA"
     gene            complement(3571..4725)
                     /locus_tag="PLD05_07045"
     CDS             complement(3571..4725)
                     /locus_tag="PLD05_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOP77229.1"
                     /translation="MKWLRLVLKLLPFVLGVVVLVLVVFWLSGFFTERVAPAQVNTEL
                     PRFDPTKETLEPVEVVIKPVIEEVVGTVKARERTTVSARIMSTITKIHVRAGDNVRVG
                     DVLVELDRAALERQRNQAQAALQAAEAALKQAEDDLSRAERLRQQQPGAISEQQFNEF
                     RTRRDQAQANYEKAKQALAEIEVNISYCTITSPRNGRIVDRLAEEGDTASPGVPLLTL
                     YDPASLRLEVPVIENVAAHLNIGDKVKVYIDALQQEIEGTVDEKVPQADVATRTVLVK
                     VSLPETPGLVEGMFGRLKIEAGSREHLCLNIQAIKRIGQLDFVEVVREDGTKEQRLIQ
                     IGRFGRPGHVEVLSGLNAGEKVAIPKRPETSESIGTDQPQCLASPASEQQ"
     assembly_gap    5370..5469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5470..7496)
                     /locus_tag="PLD05_07050"
     CDS             complement(<5470..7496)
                     /locus_tag="PLD05_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77230.1"
                     /translation="MAKSQSGGIDKILANIWEKIVLGVAILITLGLIAMSATSGPNID
                     ITPEQLTQLASRAEQNYANTKPDVIRENESFSAPRYSEVAKRIREAVSSTGYQIVAPF
                     DPPMFEQRKKRGQPPVLPVSDLRAAAGHGAVAGLGDTGSGSVGVGSGYPGYPGSSPTM
                     PTSPYPTATSTYPQMYGESETGYAGYSGYGGYGGPTGPSHGRRWVVVTGIIDWEKQIR
                     AFRDTFQDAIRVSGQGTSGMSAGMGYPGYGMGGEETYSDYSSTTGYPMTSGSPGYGYG
                     PSGTAGYVEFPRYVYFSIERAEITDESVPVDNLKWEPLNARARWFEEQRVPHDQFMYV
                     SPEYVMPPGQVPFTWPLVQLTAGDTWGKEVAHDPEIPFLEIPRGMGYGYGPYAGYPGE
                     GSPGMLGSTATGPGAAIPTGLMPGAGMAGTQPGQAAQPVQPPRPEIPDAPIAPGQMLP
                     GMPGVSPYGTSMYGTETMPEGSYTSPYGGSPYSGMYPATSGEEGYGSGYGYPGYGSGM
                     MVRKESPIKLFRFVDYKVEPGKEYRYRVKLWLANPNYKLPPQVLEDESYAREAWLLSE
                     WSTPSNVVRVPADVGVLAGPAKPGRVAFIEPRASLGVTVFQPSSGLTKFMEFADLPRG
                     TIVDFTAADVKRLNPPKREKKPTTSYPYGYPEEGMVSSETGIEGMPGMMGYP"
     gene            complement(7594..9420)
                     /locus_tag="PLD05_07055"
     CDS             complement(7594..9420)
                     /locus_tag="PLD05_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77231.1"
                     /translation="MNQLQPLLVAMKKWGFWIICGLAVVIGLGVWFIAQQSVQASIKA
                     RKDQLDGLKSQVSGVKPGSPNPKVIEAARQEIENLKRDVYQAWVTMYADQKEKNQWPK
                     ALGNEFLNWVNQPGRKFGDPIPERFREIYLNFIEQHFPTLFEIVNLRRPANVDLKQLG
                     SIRAAITAGYGASGYGSGAYPGATGTMGEEAYGETMGYPGAGGYPGTGATGTGAAAIE
                     MVGVVEWNPQNIAAIKQRFAWRQVPTSDEIWNAQEDLWVYEALLRIIAKTNEGATGPH
                     NAAVKRINAIQIGPDAAAYFASASTSAMASATGMMASPGMGSPGMEVGMPSEPSETGG
                     EYGSGYGGYGVSGMRSTFGRYVDASGNPLRPGDPQPFAEFKMMPVRLDLVVDQRKIDQ
                     LLVNCANSDMPVRITRVLIRPGQASRGGAGYGGFGGEAGMMSTGGYGYGSETGYETGA
                     SPGTGYGAGGTTQASSSHDVPIVIEGVIYLFNPPDQEQLGKQGAPELVSGQSLAPIEA
                     PPVETGDASQGGAPSQPAGAAAPQAQPGQPEPQAQPAGAQPAPAGPMAPAVPPQASQP
                     GTPTAPPSAPQGLPPAVQPAPGTGPTPVPPGTQPGQPAAANP"
     gene            complement(9480..12272)
                     /gene="pilM"
                     /locus_tag="PLD05_07060"
     CDS             complement(9480..12272)
                     /gene="pilM"
                     /locus_tag="PLD05_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOP77232.1"
                     /translation="MPKSEACWGIDIGNASLKALRLRPGESPSFVVADAFDYIEYPML
                     LTQPAADPPSLIETALRQFLSRNEVRGHKVAISVSGATGLARFIKLPPVELSKLPDVV
                     RYEAKQQIPYDLNSVVWRYQRLDAGGSESGFTLGSTVGIFALKRDQVEAAIQPFANVG
                     IEVDVIQLSPLAIFNYARFDAMPDLPPPDEYDPDNPPPSIVVISMGTDSTDLVITNGY
                     RVWQRNIPIGGSHFTKALSKELKLTFSKAEHIKKNPTTHSDPKAVFQAMRSVFTELLS
                     EIQRSISYYNTIDRAVEISRGIALGNPMKLQGLRAFLEKGLGFPLEHVDQFERLQGPD
                     VLQSPVFRENLSCFGVTYGLALQGIGRAYMEINMLPDEIVQERFIRAKKPWALAAATT
                     LLIGCTVSFAAQSLALSRVSPERFAAAERTAEQVLKTVQQYQSELASVESEFNRIKSI
                     GQNLTSSIEGRLLWLELLSAINQCLPQYRPQAGAPADVVDISAVTDTEKQQQILEQRD
                     EIHITSLEAIRTEQLETWWQRMGGWYRKPGETDTEPAETAQATAATGTATPGSPGAMM
                     GSMTSPYGMSGMYGPESETGGYGPTGGGLFASVPARTPPPSDVQGPSGSGYVIRLTGY
                     HFHNSDVAGPNQGAQYVRNTLIRNLEEGKFWINGKWVTAKELGIGYPILVNPGRIEVI
                     RVADPNYRPPAGTPGAPGTGLPTGVAPGLGTMPTGSQTYPGVTAPGVSETGTVGTAGG
                     KIPGVLTLRRFNFVVEFVWQPKTESEREKERLEQAAAAAAAQEAEAAASGGTGPATQG
                     GQPTTETAPSGGAPAPGQVVPPGVPAAVGPAAPGNGMPPQMQPGTVVPPAAGQSSVPP
                     GVTMPGVGGAMPPGAAPPTSTPVNPAEIPDAPVLPDAPVEPGAPAPGTPMTEPGQMQP
                     GETQPGQAPTGQMPNG"
     gene            complement(12647..15517)
                     /locus_tag="PLD05_07065"
     CDS             complement(12647..15517)
                     /locus_tag="PLD05_07065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP77233.1"
                     /translation="MMGDSQIRPERQNTASGGSAARTAGYRLEDQPTVITSGEILSPE
                     PATLETKGETISPTLSPGSTIGPYELLEFVGGGGMGRVFRAYDVRLSRIVALKVLTPE
                     QATDPQILARFQNEARAAARLNHPGVVQVFSAGEHAGIHYIAFEFVEGTNLRTLVEKQ
                     GPLSVPEALHYVLQVAEALDHANRRGIIHRDIKPSNIIITPEGRAKLIDLGLARVFQQ
                     NGRSQDLTTSGMTLGTFDYIAPEQARDPRLADIRSDIYSLGCTLYFLLSGQPPFPAGS
                     VLQKLLQHQSEEAPNIRAIRPDVPEAVARILRRMMAKSPRQRYQTPAELIQALRAVLG
                     VEVQTVQFGREQQRIAEKKRLPLRQHLPWLVPVVGLLLSTLVLDWWWSQGAEETIYFG
                     DDVRYTEGLFSPLTPTPPGYPAAGQPEQPQGKPTDSPIPPDRSSPSRTVPNQTELPSA
                     TSESGQVSEIGIARVRGAPVDSLESPIGSTAVSGSAFSYPQGQVAPAMGHSSGFEENK
                     VLPDATVSSQDPVPFSSSTPSDTGIPRVVTDMVTRRTPVGSEHTERFSPALSMSAEWV
                     RTVDPTTQEPRSYPTLEAACQAALPGDEIRLNFDGPLPVEGCDLRGRQLIIKAAKGRL
                     PELVFHASEQTKLRSGSFWTLAASSIVCEDVAIRIEVPPEYAADDWHLFRVSYDSTIE
                     LNRCVLTLAAARDRRQIASNDVNVGSFFLFCEDPRSALVVRAAQNREADQLRGTAVRL
                     HRCIIRGDGDLFRSTYCQPVRVTITESFVSVAGRLLDMLGTERSPASDAGVHLELQRL
                     TAWTAKGLIRQQQLTYQPYLQPIRVESEHCIFVASPNSALIEQHVPNVDGALRLLTWF
                     GQRNFYEQFSQFWLVVTGAQHSVPLQLSFDQWKAYWQTEHEQEPAWGAIPWQAPLPLD
                     VPPHLHRPSDFAIMDPSWDDIASDIANRAGCPVDQLPFVPSR"
     gene            complement(15915..16673)
                     /locus_tag="PLD05_07070"
     CDS             complement(15915..16673)
                     /locus_tag="PLD05_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOP77234.1"
                     /translation="MKRAIISDIHSNLEALEAVLKDIDSQGITEIYCLGDIVGYGPNP
                     RECIDLVMQRCKVCLLGNHDQGALFDPEGFNVGAEKAIFWTREQLEEHHGNPAESLKR
                     WDYLGERPRIHKEGPIVYVHGSVRNPLNEYVFPEDIYSPAKLQKLFSLIEKCSFQGHT
                     HTPGVFTEQCEFFSPEELPNGEFDLKSVSKAMINVGSVGQPRDGDPRACYVIQDEDRV
                     IFRRVPYNYELTCEKIYAIPELDNFLGDRLKEGR"
     gene            complement(16769..17827)
                     /locus_tag="PLD05_07075"
     CDS             complement(16769..17827)
                     /locus_tag="PLD05_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOP77235.1"
                     /translation="MDNVSRQFLTQILGNPSPSGYEQPVQEVIRTYAREYADEVRTDL
                     HGNVIAVRNPKAPFRVLLAGHCDQIGLIVQYIDDDGYIYFQSIGGWDATVLIGQRVTI
                     WTKTGPIPGIIGRKPIHLLSEEERKSVPKLKDLWIDIGAANKAETTQRVRIGDPITIE
                     LAERELLNNRIVASGLDDKSGAWVVMEALKRVDPAKLRVAAYAVATVQEELGLRGART
                     SAFGIDPQVGIAVDVTFATDCPNMEKKEEGEIKLGRGPVIYRGPNMNPKVVDRLIELA
                     ESNNIAYQLAASGRATGTDANAIQINRAGVATGLVSIPNRYMHSPVEVISLDDLDAAA
                     NLLARFVEALNPDDDFTP"
     gene            complement(18052..19674)
                     /gene="rpoN"
                     /locus_tag="PLD05_07080"
     CDS             complement(18052..19674)
                     /gene="rpoN"
                     /locus_tag="PLD05_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOP77236.1"
                     /translation="MRMSLGHELRQVQKQVQKQILSPRMIQSMEILQLPLHDLEQRVE
                     QEVIENPALEMVEIGDDEGTEDEPLEMTDTALDEPVEETSEAVDGELEDAPVTRDEDE
                     AEEPTTGGGDLEREFVVDENNSAEEFSRLVEMEREIPEIFEEENSRPSRDRIEEVSER
                     HHDLIANIPDHTETLHDHLVNQLSWYDLDPATRAMAERIISNLDENGYLLTSLEDLLG
                     PFNSEEDRERAEKALSVVQKMDPPGVGARNLRECLLLQLDPENPYYHELKLMISEYLE
                     DLEHNRIPQIAKKSKLSLETIHAALQELRRLNPRPGAQFRNEPNMAVTPDVYVEETED
                     GRFIARVVSGRIPRLQLNRTLLESLNRGNLSKEEREYIKAKIDRAKWILDGIQQRKRT
                     LQRVAQAIVDHQYRFLKEGPESIEPLKMQQIADKLGIHVTTVSRAVDDKWIQTPQGIF
                     PLRRFFCGGTASSEGEEVAWDVVRLKVKELIEHEDKTKPLSDEEIVRELARQGIQVKR
                     RTVTKYREEMGIPSSRERRDWLAIERQKREKS"
     gene            complement(19835..20434)
                     /gene="recR"
                     /locus_tag="PLD05_07085"
     CDS             complement(19835..20434)
                     /gene="recR"
                     /locus_tag="PLD05_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOP77237.1"
                     /translation="MSELTASVAQLIECLTRLPGIGRKSAERIAYHLLRVSKEEAFAL
                     ADAIRAVKENVHHCKICFNLSEQETCHICRDATRDQTLLCVVEQPQDLMAIEQTGAYH
                     GLYHVLLGRLAPLDGVGPEQLTIRELVERVRNGTFREVIMATNPTVEGDGTALFISNQ
                     LADTGVKLTRLARGITPGSVIQFANKEILTDALTGRQVL"
     gene            complement(20588..21472)
                     /gene="rsmH"
                     /locus_tag="PLD05_07090"
     CDS             complement(20588..21472)
                     /gene="rsmH"
                     /locus_tag="PLD05_07090"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOP77238.1"
                     /translation="MSNNFETQHVPVLPNEVLQWLSPQPGQIIVDGTLGGGGHTRLLA
                     ERILPGGLVIALDRDPTAVERCRQTLAGLPVRIIHADFRDLPEVLQELDIEYVDGILL
                     DLGMSSDQLMDTSRGFSFHADGPLDLRFDPSRGEPAWALLKHLSERSLADLIYRYGEE
                     RFSRRIAAAVVRERRTGGIKTARELAELISRVVPRSRSGGIHPATRTFQALRIAVNDE
                     LGALKTALERYPEVLRPGGRLVVISFHSLEDRLVKEAFLRDPRYTALTKKPITPTESE
                     IAANPRARSAKLRAARRT"
     gene            complement(21678..22250)
                     /locus_tag="PLD05_07095"
     CDS             complement(21678..22250)
                     /locus_tag="PLD05_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOP77239.1"
                     /translation="MVNVVSNNRAIAAVELSSVGVGYEVEDAMLKSASVQLLIARTIC
                     SGKYLIIVGGLVSDVQAALQAGLEAARDSVIDQLLIPNVHESVFPALGQSVVLDQGHT
                     GALGVIETFSGTAALAAADAAAKAARVTLFRIHVAMALGGKGLVLMTGSVADVRAGVN
                     AGAAEARERGLLVSEVVIPRPAPELFADYL"
     gene            complement(22314..23651)
                     /locus_tag="PLD05_07100"
     CDS             complement(22314..23651)
                     /locus_tag="PLD05_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOP77240.1"
                     /translation="MVQVTVDQVFEAGVVGAGGAGFPTYVKLSGRADTVLINAAECEP
                     LLHKDKEILRHFDDDVLAGLRIAMELVGASRGVIGIKGKYADVIEQLRPKLSPDMEIV
                     PLPDAYPSGDEFILVYEALGRVIPPGGIPLHVGAVVINVETARNVAMASRQPVVEKFL
                     TIAGAVQEPVTVRVPIGVTLAECVELAGGPTVSNPQYMVGGVMMGYLEQNHHALVDKT
                     TGGVIVLPEDHVVIRRRQQDWKQISRIGRAACDQCSFCTELCPRWLLGHPIEPHRAMR
                     SLGFNLVGEANVQGTVFCCECNLCSLYSCPEDLDPKNVCVQNKRRILAEKRRWENPPF
                     LPERAERLLKNRRAPTKRLMKKLGLTLFPNVGYLVERTVTPKMVGIKLKQHVGVPCEP
                     VVRVGDKVVRGQVVGMPPETDGKRALGAPVHASIDGVVTRIQDGVVWIERQAS"
BASE COUNT         5198 a         6966 c         6199 g         5502 t
ORIGIN      
        1 tttgcggacg gaaccgcgat ggcgccgcca cgcttcgcgt acagtaacca ataaacggtg
       61 ggaatgacca ccaaggtaaa ggtagtggaa gcgaatatcc caaagatgat ggcccaggcc
      121 aaacccgaga agatggggtc gagcgtgatc acccagttcc ccaaaagggt tgtgcccgag
      181 gtcaagatga ttggccggaa tcgcacggca acgcttctga caatggcctc cttcagatca
      241 aaaccatcca acaaactgtg gtgaataaag tcaatcagaa ccactgagtt gcgcaccacg
      301 attcccgcca gaccgatcat gccgatcatg gcggttgctg tgaaaaagac ggggttggcg
      361 aagcccccta cgggccggtt tgtgatgaga ttcagaatcc aaaatcccgg catgatgccg
      421 atcatcgtta gtggaatcgc tagcatgatg atgaggggga ggacccgtga tttggtttcg
      481 aacatcagaa ggatgaagat cacgactaag gcgaccccga aggcgatccc caagtcgcgg
      541 aagacatcga gggtgatctt ccattcgcct tctcccgtcc aaactgcctg atatccttcc
      601 ggcattgtcc agaagtcctt cccgccaggg tgaaaccacg tacgtgcctc gaccggtcgc
      661 ggctgaggtt ttttacgggg gaattcattt tccggcacca aatcgacctg catatcgatg
      721 atggcgtcgg ccggggggcg gcctgcgacc tcgccgaaga cgtaaacgac ccgtcgcaag
      781 ttcttatgat aaattgtctt atcttccaac agtggctttc cttctgggct ggttgaaaca
      841 aactttccta gagatccaag ctgtagcaat tgtccttgag aaccggggac atagagttca
      901 gccaagtcat cttcagctga acgctctgag cgtttcagtc tcaggatgac gggcagggga
      961 ttgacctcac taggaatgtg cagttcggtg gccggataac cgcccaatgc cgcttgaaca
     1021 gtctgggcaa tcatctcctt agagacgccg gaaagagccg ctttgggctg atccgtgaca
     1081 aagagcctct tttcctgatc ggcttcggca ctgatatcaa catcaacaac cccgggctcc
     1141 cggtgcatgc gcgcttcgac gaacttggcg gcgcggataa ggtcatcgta ggaagcgtcg
     1201 ggtggtccgt aaacctctgc cgtaattgtc gaaatcacag gcggcccggg cgggacttcg
     1261 acaagcttaa tgttggcgtc gatctcttgt gcaatctttg cgatctcggc gcggattctg
     1321 aggataattt cgtgtgattg ttgagcacga aaatccttgt gtagcaaatt gacccgaata
     1381 tcggcgaggt gacttccttg tcgcaggaaa tagtgtctga ccagaccatt gaagtccata
     1441 ggcgaggaca agccggcgta aatctgaaaa tctcgaactt caggcaaagt agcaagatag
     1501 gccgcgatac gtcgggcagc cgcgtccgtc tgctccagag tcgttccttc aggcatgtca
     1561 ataacgacct gaaactcatt cttgttatca taggggagca tttttagggg aacgacccgc
     1621 aagcccggta gcaagagtga cgcaacaaag agaatcgcaa cggcgaggac caccccccac
     1681 gccgtggctc gataattcaa gataggatga aagactgctc gaaccatccg gtaaatcggc
     1741 cgtttggtca aatcgtagcc ttgttcagta ctgtctccgt gttctttgga accttgcagg
     1801 gcgaacatgg ccatccacgg agtaatgacc agggacacaa tcagcgacac catgagcgtc
     1861 acggggacat tgagcgccat aggggccatg taaggcccca tcattcccgt gataaacgcc
     1921 atcggggcaa agcttgccac gatggccagg gttgacatca ccaaggcggg gcgaacctct
     1981 tgaacagcgc ggaggacggc aggccggggc ggcaaaatcc gcatggcgaa atagcgggcg
     2041 atgttttcca cgtccgtgat cggatcatcc acgagaagcc ccaatgccaa aatgagggca
     2101 aagagcgtca cccgattgat tgtgtaaccc gccagataat tgataaacaa tgtgacgcta
     2161 taacacacgg gcactgccag ggcaatgacg accgcggccc gccatccgac gaaaagccaa
     2221 atgaggccaa tcacagtcaa aacggcgacg atgagagctt ccacaagctc attgactttt
     2281 tcgttcgctg tttctccgta atcccgtgtg atgcgataga aaacattcga tggcaaatgt
     2341 gttttcgcca actgggctat gcgttcttcc actgcctgag cgacccagac ggcatttgtg
     2401 cccttttgtt tggccacggc aatcgtcacc gcggggaaat actgcagagg gatggcgcgt
     2461 ggcttggatt ttaagtcctc cggtgttaca tgctctcggg gggcctccgg atgtttggcg
     2521 gcggccccaa atcccatcca ggtgtaggag acaggctcag ccggtccgtc gatgacttgg
     2581 gcaacgtctt tcagataaac gggtcggttg cccaccacat tgacaacgag gttttccagt
     2641 tcccgggcat cgcgcacgaa cgttccggct tcaacgagga attcacggtt tcgctgttcg
     2701 taccgcccag ccctgacgtt gacgttactc accttcaggg cttgagccac ttgcagaatg
     2761 gacgtgctcc tggccgccag tttgatgggg tcaacctcaa cacggatgtg tctgggacga
     2821 ccgcctacaa tggtcacacg gttggtctgc ggaattgatt tcagctcgtt ctgcagttgc
     2881 tcggcaatcc gtcgtaactc gtagtcatga gcgtgctcgg ggtcgcggga ccatagggtg
     2941 atgttcacaa tcgggacgtc atcgatctcc acgggcttga tgacccaacc cttgacagag
     3001 gacggcgctt cgtcgatgta cgattgcact tttgcgtgga gtttgacgag cgaatcctct
     3061 cggtcttgac ccacgtagaa ccgcaccgtg acgatcgccg tttccggacg cgacatggaa
     3121 tagacatact caacgccatc aatttgatag acgattcgtt ccagccgatt tgtgatctgc
     3181 tgttcgacct cttgtgccga aagtcctggc gctgagacaa agatgtccgc cataggtacg
     3241 acgatctggg gttcttcctc acgcggggtg atccacaaag cacccaggcc gatgaggaca
     3301 gaaatgacgg tgagcatgat cgcggaatcg ccacggagaa atacatcgac gatccgtgtc
     3361 aaaaagggta ctcgctctgt cgattgagtc acgtcacgcc ctccgttatt cttttcatgg
     3421 tttgttggct gcttatcagc gtaagccctg aagtgttcac gcggtcctgc tctatcgtga
     3481 ttttctctgt gggtgtcact tttttggaca agcgaggctc cgaagaacaa tcatcaccaa
     3541 aaccaaaatt cgctgccact gacacacacc ttactgttgt tctgaagcag gtgacgccaa
     3601 acattgcggc tggtctgtcc cgatggattc gcttgtctcg ggcctcttgg ggatagccac
     3661 tttttcgcca gcatttagcc cacttaaaac ctcgacgtgt cctgggcgac caaatcgccc
     3721 gatctggata agacgctgct ctttcgtgcc gtcttctctg accacttcca caaagtccaa
     3781 ctgcccaatc cgtttgatcg cctgaatatt gaggcagaga tgttcgcgcg agccggcttc
     3841 tatcttgagc cgtccaaaca taccttccac gaggcctggc gtctccggca gcgaaacctt
     3901 gaccaagaca gttctggtgg ccacatcagc ttgggggact ttttcatcca cagtcccttc
     3961 tatctcctgt tgaagggcgt cgatatagac cttcactttg tcaccgatgt tcaaatgtgc
     4021 ggctacgttc tcaatcacag ggacctccag ccggagcgag gcggggtcgt aaagcgtcaa
     4081 gagcggcaca cccggtgacg ctgtgtcacc ctcttcggcc aaacgatcaa caatccggcc
     4141 atttctcggt gaggtgatcg tgcagtagga gatattgacc tcgatctcgg ccagggcctg
     4201 ctttgccttc tcgtaattag cctgagcctg atcgcgtcgt gtacgaaact cgttaaattg
     4261 ctgctcactg atggccccgg gctgttgctg tcggagtcgc tcggctcgac tcagatcatc
     4321 ctcggcctgt tttaaagcag cttcggcggc ctgaagggcg gcctgggctt ggttccgctg
     4381 gcgttcaagg gccgcccgat ccaattccac aagcacatca ccaactctca cgttatctcc
     4441 cgcacgaacg tgaatcttcg taatcgttga catgatcctt gcggaaaccg tggtccgttc
     4501 gcgggccttc actgttccca caacttcctc aatgacaggc ttgataacca cttcgaccgg
     4561 ttccagcgtt tctttcgtgg gatcaaatcg cgggagttca gtattcactt gcgcgggggc
     4621 gacgcgctcg gtaaagaagc cggacaacca gaagaccacg agaaccagaa caaccacacc
     4681 aagcacaaac ggaagcaact tcaagacaag acggagccat ttcatggttt tctttccttt
     4741 tccgctaagc agtgcagtcc atatagtcgt caaattaggc tgaactacgt taagtgttca
     4801 aatgacgttt gatatctgtt gcccgtgtgt ccatagcttc tcagcggcga gtcaacggcc
     4861 gaaattgcca ctggataccc accttaacag acgcaaagac tcgagaacgg gttacaaacg
     4921 agacaattcc tgatgccaca atttagcaaa ttgggctaca cagagacaag cccgacacaa
     4981 gtggtggaat caaggtcccg ccattttttt gcaccttcaa cagcttctat ggagactgac
     5041 taacatggta caagccggac ctgccggctt cctaaacgaa aaccaccttg tgggcgcaat
     5101 tggcaccaca aggtggacgt gtggcatgtg ccagatgtgg cgtggctgta attacggtcc
     5161 cggcatgggg ggcgagccca taggaccacc tcgcggagcg gtccggccgg gagtggtcgt
     5221 tcgccctctt gttgttgtgg tcccgcgcct acggtcctgg gtcccgcgcg gccctgtcat
     5281 ctgcccataa tattccgcct ccgagccata ctccgattcg tacgtcatct cgcccgggta
     5341 ccctgaatag ggcccagtcg gctgagcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnng gatatcccat catgccgggc atgccttcaa ttcccgtctc gctactgacc
     5521 attccctctt cgggataacc gtacggatag gacgtggttg gttttttctc gcgcttcgga
     5581 gggttcaatc gcttcacgtc tgctgccgtg aaatcgacaa tggttccgcg gggaaggtcg
     5641 gcaaactcca tgaactttgt gagtccactg ctaggctgaa ataccgtgac ccccaaagag
     5701 gccctgggtt cgataaaggc cacacgcccc ggtttagccg gtcctgccag gacgccgaca
     5761 tcggcaggta cccgcaccac gttggagggg gtgctccact cgcttagcag ccacgcttct
     5821 cgtgcgtagc tttcatcctc caagacctgg ggaggcaatt tataattggg attcgccagc
     5881 catagtttga ctcgataccg atactctttg ccgggctcaa ctttgtagtc aacgaagcgg
     5941 aacaatttga ttggtgactc tttgcgaacc atcattccgc tgccatatcc ggggtatcca
     6001 tagccgcttc cgtaaccctc ttcacctgag gttgccggat acatcccgga atagggcgag
     6061 ccaccgtacg gcgacgtata actgccctct ggcatggtct ccgtgccata catggacgtt
     6121 ccataaggtg acacgccggg cattcccggt aacatttgtc cgggagcgat cggggcatct
     6181 ggaatctcgg gacgaggcgg ttgaaccggc tgggccgctt gtcccggttg cgtgcctgcc
     6241 atcccagctc ctggcataag gcccgtgggg atcgccgcgc ccggacccgt cgcagtgctt
     6301 cctagcatgc ctggcgagcc ttcgcccgga tagcctgcgt acggtccata gccgtagccc
     6361 attccacgcg gaatttctaa gaacgggatt tcggggtcat gtgccacttc tttgccccag
     6421 gtatctcctg ccgtcaactg aactaatggc caagtgaaag gcacctgacc cggtggcatg
     6481 acgtattcag gcgaaacgta catgaattga tcatggggga cccgttgttc ttcaaaccac
     6541 cttgcacggg cattcagggg ttcccatttc agattgtcca caggcaccga ctcatccgtg
     6601 atctcggccc gctcgatgga gaagtacacg tagcgaggaa attccacata accggcggtc
     6661 cccgaaggac cgtaaccgta cccaggagac cctgaagtca tcggatagcc cgtggtgctc
     6721 gagtaatctg aatatgtttc ctccccaccc attccgtatc cggggtatcc catgcccgca
     6781 ctcatccccg acgtaccttg tccggatacc ctgatagcat cctggaatgt gtctcggaat
     6841 gcccgaatct gtttttccca atcaatgatt cccgtaacca caacccaccg acgaccgtgg
     6901 ctcggccccg taggtccccc atatcctccg tatcccgagt acccggcata gcccgtctct
     6961 gactctccgt acatctgggg gtaggtggaa gtcgctgtgg gatagggaga agttggcatg
     7021 gtggggctac tgccgggata tcctggatag ccactcccaa caccgaccga cccagatccg
     7081 gtatccccta gacccgccac agcgccgtga ccggctgccg ccctcaaatc ggaaacgggc
     7141 aagacaggcg gctggccgcg tttctttctc tgctcgaaca tcggtggatc gaacggggcg
     7201 acgatttgat atccggtgga agagacagct tccctaattc gttttgccac ttccgaatac
     7261 ctgggcgctg aaaaactttc attctctctg atcacgtcgg gtttggtgtt ggcgtagttc
     7321 tgctccgcac gactggcaag ctgggtcaat tgctcaggcg taatgtcaat gttagggcca
     7381 ctcgtggccg acatcgcgat taatcccaac gtgataagaa tcgcaacgcc aagtacgatc
     7441 ttttcccaga tgttggccag aatcttatca attccaccgg attgtgactt ggccattttc
     7501 aatccctcgc aacaaaggcc cacacacttg ggtcaggact tttcctctcg gttagccaat
     7561 cggacttttg ctcccgctat catcatacct tcactatggg tttgctgctg caggttgccc
     7621 tggctgtgtc ccaggcggaa cgggtgttgg gcccgtacca ggtgccggtt gcactgctgg
     7681 cggcaaaccc tgcggcgcac tgggcggtgc tgttggggtt cccggctgac ttgcctgggg
     7741 cggtactgcc ggagccatcg gccccgctgg cgccggttga gccccggccg gttgagcctg
     7801 tggttcaggc tgaccaggtt gagcctgtgg ggcagctgct ccagcgggct gtgaaggggc
     7861 gccaccttgt gatgcgtccc ccgtctcaac tggcggagct tcgataggcg ccaatgattg
     7921 tccggagacc aactcaggag ccccttgttt tccaagttgt tcctgatccg gaggattgaa
     7981 taggtaaata acaccctcga tcacgatggg gacatcgtgc gatgatgaag cctgggtggt
     8041 tcctccggct ccatatccag ttcccgggga tgcaccagtc tcataacctg tctcgcttcc
     8101 gtatccataa ccgccggttg acatcatccc cgcctcgccc ccaaatcctc catatccagc
     8161 tccacctcga ctcgcttggc caggcctgat caagacccgt gtgatacgca ccggcatatc
     8221 ggagttcgca cagttgacga gtagctggtc aatcttacgc tgatcgacga ccaagtccaa
     8281 gcggaccggc atcatcttga attcggcaaa tggttgggga tcgccggggc gaagcggatt
     8341 acccgatgca tcaacataac ggccaaacgt ggaacgcata cccgatacgc cataaccgcc
     8401 gtagccgctt ccgtattcac caccggtttc gcttggctca ctcggcatac caacctccat
     8461 gccgggggaa cccataccgg gagaggccat catccctgtg gcacttgcca tcgcagatgt
     8521 gcttgctgaa gcgaaatacg ccgctgcatc gggcccgatc tgaatggcat tgatccgttt
     8581 aacggctgcg ttgtgtggcc ctgtcgcgcc ttcatttgtt ttggcaataa ttcgcaaaag
     8641 tgcctcgtaa acccacaaat cctcttgtgc gttccatatt tcgtcagacg tggggacctg
     8701 ccgccatgcg aatcgctgtt tgatggctgc gatattctga ggattccatt ccacgacacc
     8761 caccatttcg atagcagccg ctccggtacc tgttgccccg gtaccgggat agcctcctgc
     8821 ccccggatat cccattgttt ctccgtaggc ttcttcaccc atggtgcccg tagcacccgg
     8881 atatgcacca ctcccataac ctgatgcccc atatccagct gttatcgctg cccgaatact
     8941 tcccagttgt ttaaggtcca cattcgcagg tcgccgtaaa ttgacgattt caaataaggt
     9001 cggaaaatgt tgttcgatga agttgagata gatttcccgg aaccgttctg ggatgggatc
     9061 accaaacttt cgcccaggct gattcaccca atttaagaat tcatttccca acgccttagg
     9121 ccactgattc ttttcttttt gatcggcata catggtaacc caggcctggt agacgtcgcg
     9181 cttgaggttc tcaatttcct gccgagctgc ctcgatgact ttaggattgg gcgaacccgg
     9241 tttaacgccg ctgacctggg acttcaggcc gtccaattgg tccttgcgcg ccttaatgct
     9301 cgcctggaca gactgctggg cgatgaacca cacgcccaaa cctatgacga cagcaaggcc
     9361 gcatattatc caaaaacccc attttttcat ggcgacgagc aggggttgaa gttggttcat
     9421 atcacaaatc ctctcatgct gagtggactt ttaagctgaa tcacatttct ctctcgttac
     9481 taaccgttgg gcatctgtcc cgtgggcgcc tgtcccggtt gtgtctcacc cggctgcatt
     9541 tgtcctggtt cagtcattgg agtccctgga gctggagcgc ccggttcgac tggggcgtca
     9601 ggcagaacag gtgcatccgg tatttccgct gggttcacag gagttgaagt tggaggcgct
     9661 gcgccgggtg gcatagcgcc gcctacgcct ggcatagtga ctcccggagg aacactggac
     9721 tgacccgctg ctggtggtac tacggtaccg ggctgcatct gtggaggcat gccgtttcca
     9781 ggagctgctg gccctacggc cgccggtacc cctggaggca ccacctggcc aggggcagga
     9841 gcgccaccgc ttggcgccgt ctcggttgtc ggttgaccac cttgtgttgc tggtccagtt
     9901 ccgccacttg ctgcggcctc tgcctcttgg gctgccgcag cggcagccgc ctgttccaat
     9961 cgttctttct ctcgctctga ctcagtcttg ggctgccaaa caaactcgac aacaaagttg
    10021 aatctccgta atgttaatac tccggggatc tttccgcccg ctgtaccgac ggtccctgtc
    10081 tcggaaacac cgggtgcggt gacaccggga taggtttggc tgcctgtggg cattgttcct
    10141 agcccaggag caaccccggt gggtaacccg gtacctgggg cacctggcgt cccggccggc
    10201 ggccgataat ttggatcggc cacacggata acctcgatcc gcccagggtt gaccaagatg
    10261 ggatagccaa tccccaattc cttggctgtt acccactttc cgttgatcca gaattttcct
    10321 tcttcgagat tccgaatcag ggtattcctt acatattgcg caccctgatt gggtccagcg
    10381 acatcgctat tgtggaagtg ataacccgta agtcgtatca catatccgct tcccgaaggc
    10441 ccttgcacat cggagggagg tggtgtgcgg gctggtacgc tcgcaaatag tccccctccc
    10501 gtaggcccgt agccgcctgt ttcgctttcc ggtccgtaca ttccggacat tccatatggg
    10561 gaagtcatcg accccatcat cgctcccgga ctgcccggtg ttgctgttcc cgtggccgcc
    10621 gtcgcttgag ctgtctccgc aggctcggtg tctgtttctc ccggcttgcg ataccatccg
    10681 cccattcgct gccaccacgt ttcaagttgt tctgttcgga tggcttcaag gctagtgatg
    10741 tggatctcgt cccgttgttc caagatctgc tgttgctttt cggtatcggt aaccgccgaa
    10801 atgtccacga cgtccgcggg agccccagct tgaggacgat actgtggtaa gcattggtta
    10861 atggcagaaa gcaattcaag ccagaggaga cgaccttcaa tactactcgt caggttttga
    10921 ccaatgctct tgatcctatt gaactccgac tctaccgagg cgagctccga ctggtattgt
    10981 tgtaccgtct tcaaaacctg ctctgccgtt cgctcggctg ccgcaaatcg ttccggtgaa
    11041 acccggctca aagcaagaga ctgcgcagcg aaactgaccg tgcatccaat caggagagtt
    11101 gtagccgctg ccaatgccca cggcttctta gcccgaatga atcgttcttg tacgatttca
    11161 tccggtaaca tgttgatttc catgtaggcc cgaccgattc cctgtagggc cagcccataa
    11221 gtaacgccga aacacgacag gttttctcgg aacacaggag attgcaagac atcgggcccc
    11281 tgcaagcgct caaactgatc cacgtgctcc aggggaaatc ccagcccttt ttcgagaaaa
    11341 gcccgcaacc cctgaagctt catggggtta cccaaggcaa ttccccgcga aatctcaaca
    11401 gcccgatcta tcgtattgta gtaactgatc gaccgctgga tttcggagag caactccgta
    11461 aataccgagc gcattgcctg aaacactgcc ttggggtctg aatgggtcgt gggattcttc
    11521 ttaatgtgct ccgccttgga gaacgtcagc tttagctcct tactcaaggc ctttgtaaaa
    11581 tggcttccac caatcgggat attccgctgc cacacccgat agccattggt gattaccaag
    11641 tcggtgctgt ccgttcccat ggaaatcaca acaatggacg gtggggggtt atccgggtcg
    11701 tattcgtccg ggggcggtaa atctggcatc gcgtcgaagc gagcatagtt gaaaatcgcc
    11761 aatgggctca actggatcac atcgacttcg atgccaacgt tggcgaaagg ctgaatagcc
    11821 gcttccacct ggtcccgctt gagtgcgaaa atccccaccg tggatcccaa ggtgaagcca
    11881 ctctcgctgc cccccgcatc cagtcgctgg tagcgccaca cgacgctatt taagtcatac
    11941 ggaatttgct gcttggcttc atatctgacg acatccggca gcttgctaag ctccacaggg
    12001 ggtagcttaa taaaacgtgc aagccccgtc gctcccgaga cagagattgc gaccttatgg
    12061 ccacggacct catttcgtga caaaaactgg cgaagggctg tttcgatcaa actcggagga
    12121 tccgccgccg gttgcgtgag aagcatcgga tactcgatgt agtcaaaggc gtcggcaacg
    12181 acaaagcttg ggctttcgcc aggtcgcaac cgtagcgctt taagcgatgc attcccgata
    12241 tctattcccc agcaggcttc tgactttggc atagacgaca cctcatttat gggaccgctc
    12301 ggccaccaag cccttttcct attgctcaga agaccctgtt acccatgtcc cctgattaga
    12361 cggtccgtct ctccctgcta ccgaaaagct ccccgatctg tccaaaaaaa ttactatcat
    12421 tccacccctt aatgtatccc cgcgcacccc caggtgtcaa cttgcaatcc gcttccgaag
    12481 cgatttcccg caacgtttgc ccctaccggc tttccggccg ccattcgcac catcgaatgt
    12541 gacgtcacag ctgttcactg aaggaagaat cgccgacaag tttattgtgc acccacaatt
    12601 aatgtctttt aatctcccta ccggtcttcc gttgaagaaa acggcgctat cgtgacggga
    12661 cgaaaggcag ttggtccacg ggacaaccgg cacgattcgc aatgtcgctc gcgatgtcat
    12721 cccacgaggg atccataatg gcaaaatcgc tgggtcgatg gaggtgagga gggacgtcca
    12781 gtgggagagg ggcctgccac ggtatggcac cccacgcggg ctcctgctcg tgctcagtct
    12841 gccaataagc cttccactga tcaaacgaca gttggagggg gacagaatgc tgagctcctg
    12901 tcaccaccag ccagaattgg ctaaattgct catagaaatt acgctgcccg aaccatgtca
    12961 ataagcggag ggctccatcc acattcggca cgtgctgctc aatcaaggca ctattgggac
    13021 tggcgacgaa gatgcagtgt tcactttcca cccggatcgg ctgaagatag ggctgataag
    13081 ttaactgctg ctgccggatt agtccttttg ccgtccaagc ggtcagacgt tgcaattcta
    13141 ggtgcacgcc cgcatcggac gcaggcgacc gttccgttcc cagcatgtcg agcaaccggc
    13201 ctgccacgga gacaaagctt tccgtaatcg tcacccgtac cggctggcaa tatgtgctgc
    13261 ggaaaagatc gccatctccg cggataatgc accgatgcaa ccgaaccgca gtcccgcgca
    13321 gctgatctgc ttctctgttt tgtgcagccc gaacaacgag cgcactccgt gggtcttcac
    13381 aaaagagaaa aaaactaccg acattgacgt cgttagacgc tatctgtcgc ctgtcacgcg
    13441 cggcggccaa ggtcagtaca cagcggttca actcgatggt tgaatcgtag ctcacgcgaa
    13501 aaaggtgcca gtcgtctgcc gcgtactcgg gaggcacctc aattctgatg gcaacgtcct
    13561 cgcaaacaat ggaagaggcc gccaatgtcc aaaaggaacc gctacgcagc ttggtttgct
    13621 cggacgcgtg aaacaccaat tcgggcaatc gccctttcgc tgccttgata atcaattgcc
    13681 gcccgcgcag gtcacaaccc tcgaccggta acggcccatc aaaattaagc cgaatttcgt
    13741 cacccggaag agccgcctgg catgctgcct cgagggtcgg ataactccta ggttcctgtg
    13801 tggtgggatc gaccgtccga acccattcgg ccgacatcga tagggctggt gaaaatcgtt
    13861 ccgtgtgttc gctgccgacc ggagtcctcc tcgttaccat gtccgtgacc acgcgaggaa
    13921 tgcccgtgtc agatggagtt gaggaggaga acgggaccgg atcctgggaa gagactgtgg
    13981 cgtccggaag gactttgttt tcctcaaaac cgctcgaatg gcccattgcc ggtgcgactt
    14041 gaccttgagg atatgaaaaa gctgagccgc tgaccgccgt cgaaccaata ggactttcca
    14101 aactgtctac cggagcaccc ctgacccgcg ctatcccgat ttccgatact tgtccggact
    14161 cactcgtggc agaaggcagc tcggtttgat tgggcaccgt gcgagaaggt gatgatctgt
    14221 ctggagggat aggcgaatcc gtgggtttcc cttgcggctg ctccggttgc ccagccgccg
    14281 ggtagcccgg gggcgtggga gtcagcgggg agaagagacc ttcggtatat cggacatcat
    14341 ccccgaaata gatcgtttcc tccgctcctt gggaccacca ccaatccaga acgagcgtac
    14401 tcaaaagcaa acccacgacc ggtaccagcc atggtaaatg ctgtcgtaac ggcagacgtt
    14461 tcttttctgc tattctttgt tgctcacgac caaactgaac agtttgaact tcgacgccaa
    14521 gaacggcgcg gagcgcctgg atcaactccg cgggcgtttg ataccgctgc cgcggagact
    14581 tggccatcat ccgtcgcaga atcctggcca ccgcttctgg tacgtccggt cgaatcgctc
    14641 ggatatttgg cgcctcctcc gactgatgtt gcagcaactt ttgcaggacc gaaccggcag
    14701 gaaacggggg ttgccccgac aagagaaaat aaagcgtgca tcccaacgaa tagatgtcgc
    14761 tccgtatgtc cgccaaccgt gggtccctgg cttgttctgg agcaatataa tcaaatgttc
    14821 ctaatgtcat gccgctcgtc gtaaggtcct gcgaacggcc attctgttgg aacactcgcg
    14881 ccagaccaag atcgatcagc ttagcacgac cctccggtgt tatgatgata ttggatggtt
    14941 tgatatctcg gtggatgata ccgcgacggt tggcgtgatc aagggcttct gctacctgta
    15001 gcacgtaatg gagggcttcg ggcacggaaa gcggtccctg cttttcaacc aacgttcgta
    15061 ggttcgtgcc ctcaacgaat tcgaaggcaa tataatgaat cccggcatgc tcaccagcgg
    15121 agaacacttg gacgacgccg ggatggttca agcgagcggc cgcccgggct tcgttttgga
    15181 accgcgcaag gatttggggg tcggtggctt gctccggggt taagaccttg agcgcgacaa
    15241 tgcgagatag ccggacgtcg tacgcccgga atacccgccc catccccccg ccgccgacga
    15301 attccaggag ttcataggga ccgattgtac ttcccggcga aagagtaggg ctgatcgttt
    15361 cgcccttcgt ttccaatgta gcaggctcag gggaaaggat ttcaccgctc gtaataactg
    15421 taggctgatc ctccagccga tatcccgcgg tcctcgcggc gctaccgccg gatgcagtgt
    15481 tttgcctctc tggtctgatt tgggaatcac ccatcatcag tctctcaaga ctctttcagg
    15541 actccacgag ccccggatga gaatccttac cggcgccact gccctcttat cgttctggaa
    15601 aagcaaaagt taccaatgaa ctagacaatt tggactagat gatcccccac aaatggctgg
    15661 ggatcgggac tatcaccttc cgtacgaagc ctcttgcgct gctattagcc tcccctatta
    15721 ggaggagcat tacctcatta taagcgcaaa aggtaaaccc ggtcaaacag actcttcttt
    15781 ccggtagccc ggcggttaca tcggagtaga agccgctctg ctttatacca agctcgcttg
    15841 ttgtaaaatt cttgcccctg gtatgcaaag aaaacagcgg tcgtctcttt cgcgaggttc
    15901 ttggagtgat tcggctagcg gccctctttc agccggtcgc cgaggaaatt atccaattct
    15961 gggatggcat agatcttctc gcacgtcaac tcgtaattgt agggcacccg tcggaagatc
    16021 actcggtcct cgtcttgaat cacgtaacac gcccgagggt ccccgtcccg tggctggccc
    16081 acggaaccca cgttgatcat cgcctttgaa acggatttga gatcaaattc accgttaggc
    16141 aattcttccg gcgaaaagaa ttcacactgt tcggtaaaca caccgggcgt atgcgtgtgt
    16201 ccttgaaagg agcacttttc aatcagtgag aagagctttt gaagtttcgc tggcgaatag
    16261 atgtcttctg gaaagacata ttcgttcaac gggttgcgca cggagccatg aacgtacacg
    16321 atgggcccct ccttatggat gcgggggcgt tccccgagat aatcccatcg cttcaacgat
    16381 tcagccggat ttccgtggtg ctcctccagt tgttcacggg tccagaaaat ggccttctcg
    16441 gcgcccacat tgaatccttc gggatcgaag agagcccctt ggtcatgatt tcccaggagg
    16501 cataccttgc agcgttgcat caccaggtcg atgcactccc gcggattggg accataccct
    16561 acgatatcgc cgagacagta aatctcggtg attccctgag aatcgatgtc ctttaaaacc
    16621 gcttccagtg cctcaagatt gctgtgaatg tcgctgatga tcgctcgttt caccgatggt
    16681 caccatttct ttatgacgtg aaacctaaac ctttctttca gccgaaatct ccaaatccgt
    16741 gcttataact cggctaacaa gctcaccatt atggagtgaa atcgtcatca ggattcaggg
    16801 cctcaacaaa ccgagccaga aggttggctg cagcatccaa atcgtcaagc gaaatgactt
    16861 ccacagggct atgcatatag cggttgggaa tgctcaccaa cccggtggcc acacccgccc
    16921 ggttaatttg aatggcattg gcatctgtgc ctgtcgccct ccctgaagct gccagttgat
    16981 aggcgatgtt attgctctcg gccaattcaa tgaggcgatc gaccactttg ggattcatat
    17041 tcggcccgcg gtaaatgacc ggcccccgcc ccaacttgat ttcgccttct tccttctttt
    17101 ccatgttggg gcaatccgtg gcgaaggtaa catcgacggc aatgcccacc tggggatcga
    17161 tcccaaaggc acttgtccgt gcaccccgta atcctagctc ctcttgcacg gtagccacgg
    17221 cataagcagc aacccggagt tttgccggat caacacgttt gagggcctcc attaccaccc
    17281 atgcaccaga cttgtcgtcg aggcccgacg ccacgatccg attatttagg agttcccttt
    17341 ccgccaactc aatggtgatc ggatcaccaa tacggactct ttgcgttgtc tctgccttat
    17401 tggcggcgcc aatgtcaatc cacaggtctt tcagtttggg taccgacttc cgctcttctt
    17461 ccgacaacaa atgaatcggt tttcgaccga tgatgccggg aatggggcca gttttcgtcc
    17521 aaatcgtcac ccgctggcca attaacacgg tcgcgtccca accaccgatg gactggaaat
    17581 agatgtagcc atcatcgtcg atgtattgaa cgatcagccc aatctgatca caatgcccag
    17641 ccaaaaggac tcgaaaaggg gctttgggat tccggacggc aattacgttt ccgtgcaaat
    17701 ccgttctgac ttcgtcagcg tactccctgg catatgttcg aatgacttct tgaacaggtt
    17761 gttcatagcc tgaaggactt ggatttccga gaatctgtgt gaggaactga cgtgagacat
    17821 tatccatcga tgtcgctcca atttcttctt tcttcccgtg acgcttagtg gccgagacga
    17881 acttggcatt ccttaaccca gggcatgaat ccgaatcgtg tattatagaa cacgatacgc
    17941 ggcggttggc aactcccaac ggacgcacta cgacggattt tcgcgagcca gagaaagaac
    18001 gtggccacag ccccgtccat ctcttccggc aggagggctt gcccgtgaca ttcagctctt
    18061 ttcgcgtttc tgccgctcaa tggcgagcca atcacgtctt tcgcgtgagc tgggaatccc
    18121 catctcttct cggtactttg tgaccgttcg ccgcttgacc tgaatcccct gccttgccaa
    18181 ttctctcacg atttcttcgt cactgagcgg cttggtttta tcttcgtgct cgatcagctc
    18241 tttcactttt aagcggacca catcccaggc aacttcttcg ccctccgaac tggccgtccc
    18301 gccacagaag aatctccgta aggggaagat accttgcggg gtctggatcc atttgtcgtc
    18361 cacggcacga ctgaccgtgg tgacgtggat tcccaacttg tcggcaatct gctgcatttt
    18421 taggggttcg attgattcgg gcccttcctt caaaaatcgg tactgatggt ccacaatggc
    18481 ctgggccacg cgttgcaggg tccttttcct ttgttggata ccatccaaga tccacttagc
    18541 ccggtcgatt ttcgccttga tatattctct ttcttccttg gagaggttac cccgattcag
    18601 gctttccaaa agtgtccgat taagttgcaa ccggggaatt ctgccggata ccacccgtgc
    18661 gataaaccgt ccgtcttcgg tttcttccac gtacacatca ggagtaaccg ccatgttggg
    18721 ctcattgcgg aactgggcac ctgggcgggg gttgagtcgg cgtaactctt gaagcgcggc
    18781 gtgaattgtc tcaagagaga gtttcgactt cttggcaatt tgcggaatcc gattgtgttc
    18841 taaatcctca aggtactcgc tgatcatgag cttcagctca tgataataag ggttttcggg
    18901 atcgagttgc agcagaagac actcacggag gtttctcgcg cccacacccg gcgggtccat
    18961 cttttggacg acagagaggg ccttctccgc ccgctctctg tcctcctcgc tgttaaacgg
    19021 acccaatagg tcttcgaggc tggtcagcaa atacccattt tcatcgagat tggaaataat
    19081 gcgctctgcc atcgcccgcg tggcgggatc caagtcatac caggacaatt gattcaccaa
    19141 atggtcatgc agtgtttcgg tatggtcggg aatattggcg atcaggtcgt gatgacgctc
    19201 cgagacctct tcgatccgat cgcgcgacgg ccgggagttt tcctcctcaa aaatctcagg
    19261 gatctcccgt tccatctcga cgagccggct aaattcttct gccgagttgt tctcgtcaac
    19321 cacgaactcc cgttccaagt caccgccgcc cgtggtcggc tcttccgctt catcctcgtc
    19381 acgagttact ggcgcgtcct ccagttcacc gtctaccgct tccgaggtct cctccaccgg
    19441 ttcgtccagg gcggtgtccg tcatctctag cggctcgtcc tcggtgcctt cgtcgtcgcc
    19501 gatttccacc atttccaaag ctgggttttc gataacctct tgctcaacgc gctgttcgag
    19561 atcatgaagt ggtagctgga ggatctccat cgactgaatc attcgcggcg acaggatctg
    19621 cttttgaact tgcttctgaa cctgtcgaag ttcgtgtcct aatgacatcc gcataactgt
    19681 gacctcacac gcagtggaaa ctcggccata gtacggtcca atccggtttc tcccagatca
    19741 gacgcatgca tccttcagtt gaaatctacc acctccgcag cacaaaaacc aaaagtggca
    19801 acgacctcgt cagttttttg ccatttccat ttcgtcaaag aacctgtctg cccgtcaagg
    19861 catccgtcaa aatttccttg ttcgcaaatt gaataacgct accgggggtg attcctctcg
    19921 ccaaacgcgt cagttttaca ccggtgtcgg caagttggtt agaaataaac agggctgtcc
    19981 cgtctccctc cactgtgggg ttcgtcgcca tgattacctc tcgaaatgta ccatttcgga
    20041 ccctctcgac caactcccgg atggttagtt gttcggggcc gacgccgtcg agtggtgcga
    20101 gtcgccccag gagcacatgg taaagaccat ggtaagcccc tgtttgctca atggccatca
    20161 gatcttgggg ctgctccacg acgcaaagta gagtctgatc ccttgtcgca tcgcgacaaa
    20221 tgtgacaggt ctcttgttcg gagaggttga agcaaatctt acaatgatgc acattttcct
    20281 tgacggcgcg aatcgcgtcc gcgagagcga aagcctcttc cttagagacc cgcagcaggt
    20341 gataggcaat tctttcggcc gactttcgtc cgataccggg aagtcgcgtt aaacactcaa
    20401 tcagttgggc aactgatgcc gtcaattcac tcacgtctca cctcgcagtt caaagcgctc
    20461 ccccctgatg gcagcccact ccccccacaa tccacccgga acaggctgca tcacctgcaa
    20521 aagaattcta caagatagcc gttgattata gaactccgcg ctgcggttgg gcccccgggg
    20581 aaccgcgcta ggtccggcgt gccgctcgca gttttgcgct tcgcgcgcgg ggattagcgg
    20641 cgatttcact ttctgtgggg gtaatcggtt tcttcgtcag agcggtgtaa cgaggatcac
    20701 gtaagaaggc ctctttgacg agacgatctt ccagcgaatg gaaactaatg accaccaagc
    20761 gtcccccggg tcgtaacacc tccgggtacc gttcaagtgc tgtcttcagg gcccctagtt
    20821 catcgttgac agcgatacgg agggcctgga acgtcctggt tgccggatgg ataccccctg
    20881 agcggctccg cggcaccacg cgcgaaataa gctctgccaa ttcgcgagcc gtcttgatcc
    20941 cgccggtacg gcgttccctg accacggccg ccgcaattct ccgtgaaaag cgttcctcgc
    21001 catatcggta aatcaaatcg gcgagcgacc gttcgctaag gtgcttaagc agtgcccagg
    21061 ccggttctcc ccgagatgga tcaaatcgta agtcgagcgg gccatcggca tggaaactga
    21121 acccgcgaga ggtatccatt agctggtcgc tagacatgcc aaggtcgagg agaatgccat
    21181 ccacatattc gatatccaac tcttggagaa cctcgggcaa gtcacgaaaa tccgcgtgga
    21241 ttatgcgaac gggcaagccg gccaaggttt gccgacatcg ctccaccgca gtggggtctc
    21301 ggtcaagtgc aatgaccaaa ccaccgggga gaatccgctc ggctaacagc cgcgtgtgtc
    21361 cacctccgcc caaagtgccg tcgacgataa tctgccccgg ctgaggagac agccactgga
    21421 ggacctcgtt gggcagcacg gggacatgtt gagtttcaaa attgtttgac ataacgcaca
    21481 acgcatccta ccagcgcgca cagggcatcc aagcatattc tacgcgagct gcttttcact
    21541 tgtcgtaacc agcttgcgcc aaaggcgata ggtcccgcgg gttcaatctg agtgttaaca
    21601 accgatcgcc catagcctcc gttcgttatg ccagagtaag ccacggaatc cgaccgcttt
    21661 tggtacagga ataaacccta taggtaatct gcaaaaagtt cgggtgccgg tcgtgggata
    21721 accacttcag aaacaagtag ccctcgctct cgcgcctcgg ccgccccggc attgacaccc
    21781 gcccgaacgt cggcgaccga ccctgtcatg agcaccaacc ctttgccgcc caaggccatt
    21841 gccacatgga ttcggaagag ggttacccgt gcagcttttg ccgccgcatc ggccgccgcc
    21901 agggccgcag ttccactaaa tgtttcaatc acaccgagtg cccccgtatg gccttgatca
    21961 agcaccacag actgcccaag ggccgggaac acggactcat ggacattcgg gatgagcagt
    22021 tggtcaatga ccgaatccct agctgcttcc aagcccgcct gtaaagccgc ctgaacgtcg
    22081 cttactaacc caccaacgat gatgaggtat tttcccgagc agatcgttcg cgcaatgagg
    22141 agctgaacgg acgccgattt gagcatggca tcttccacct cgtagccgac gcctacactg
    22201 gacagctcaa ctgccgcgat cgctcgattg ttggaaacca cattaaccat aagcaccacc
    22261 tagattgagt taatgacttc ctgcgaaacc agaatccgct agttaacgct cactcacgag
    22321 gcctgtcgct cgatccacac cacaccatcc tgaattcgtg tcaccacacc atcaatcgat
    22381 gcatggacgg gtgcaccaag cgcccgttta ccgtcggtct ctgggggcat tcccaccacc
    22441 tgaccgcgaa cgaccttgtc tcccaccctt acaaccggct cgcacggcac gcccacgtgt
    22501 tgtttaagct tgatgccgac catcttcgga gtaacggtcc tttctaccag gtaacccaca
    22561 tttgggaaca acgtaagccc gagctttttc atcaatcgct tggtcggggc cctgcgattt
    22621 tttaggaggc gttcagcgcg ctctggaaga aagggaggat tctcccagcg gcgtttttcg
    22681 gcgaggatgc ggcgcttgtt ttggacacaa acattcttgg gatcaaggtc ttccggacaa
    22741 gaatataggc tacaaagatt gcactcacag cagaacaccg tcccttgcac attggcttcg
    22801 ccaaccagat taaatccgag gcttctcatc gcacgatgtg gctcaatggg atgacccaag
    22861 agccatcgcg gacataattc cgtacagaaa ctgcactgat cacaggcggc ccggccaatc
    22921 cgcgaaatct gtttccaatc ctgttgcctt cgccgtatca ccacgtgatc ttccggcagc
    22981 acgatcacgc cgccggtggt tttatctacc aacgcgtggt gattttgttc taggtaaccc
    23041 atcatcacgc cgcccaccat atactgcggg ttggagacgg tcggacctcc ggcaagctca
    23101 acgcactccg ccaaggtcac gccaataggt acacggaccg tcacaggttc ttggacggcc
    23161 ccagcgatcg tcaggaattt ttccaccaca ggctgacgac tagccattgc cacgttccga
    23221 gccgtctcca cattgataac cacggccccg acgtgaaggg ggataccacc cgggggaatc
    23281 acccgaccta gggcctcata gaccaagata aactcatcgc cggaaggata tgcatcaggt
    23341 aggggcacga tttccatatc cggggacagc ttcggccgga gctgctcgat gacatcggcg
    23401 tacttgccct taatcccaat gacgcctcgc gatgccccca ctaactccat ggcgattcgc
    23461 aaaccagcca agacatcgtc gtcaaaatga cgcaggatct ccttgtcctt atgtaaaagt
    23521 ggttcacatt ctgccgcgtt aatcaaaaca gtatcggcgc gacctgataa tttgacatat
    23581 gtcggaaagc cggcaccacc cgcaccgacc accccggctt cgaaaacctg atcgacggta
    23641 acctgaacca ttaccaaaat cccttacatc aagtcctggc aaaaaagacg cgaagcgtgc
    23701 tggaccgcgg tcgcctgtcc attggacgcc ccttcaaata cctgaatatg accggcaaag
    23761 tctatcttct cgacaatcat agctcatatc acgtgtgttc taaagtccat cagagcgagc
    23821 gcattttacc gcaaacgccg taaccaccgg cgccgatact caatgtttgt attttcgaag
    23881 gaccttttcc cagattgaga tgggttgttg ccattcaagg agttgaggag tatcccaatg
    23941 tgctgatgcc caaggagtgg tcgag
//
LOCUS       DAOSUT010000058        23788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1392_length_23788_cov_9.433700, whole genome shotgun sequence.
ACCESSION   DAOSUT010000058 DAOSUT010000000
VERSION     DAOSUT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 23788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23788
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1392_length_23788_cov_9.433700"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLD05_07105"
     CDS             complement(<1..716)
                     /locus_tag="PLD05_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77241.1"
                     /translation="MFTRSPRRRFLKISAALASAPFAATIVPSFAESPNEKLNIAAIG
                     VRGRGAANLAGVASENIVALCDVDANNLNEAAKDFPNAKLYRDFRRMFDEIAKEIDAV
                     VVSTPDHTHAPAAAAAMQLGKDCYCEKPLTHCVYETRYLTELAIRNKLVTQLGTQIHA
                     GDNYRRVVELVKANVIGTISEVHVWSAASYSGGDRPKDTPPVPPYLDWDLWLGPAPYR
                     PYHPAYVPGRWRGWKDFGNGG"
     gene            complement(918..1412)
                     /gene="greA"
                     /locus_tag="PLD05_07110"
     CDS             complement(918..1412)
                     /gene="greA"
                     /locus_tag="PLD05_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOP77242.1"
                     /translation="MDERIPVTQAGYEKLCRELRELEDQLAEVTKRVAAAREEGDLRE
                     NAEYHGARETQGLVMAKINYLRDKIARCEIVDPSRIPRDRVALGCTVTVRDLDKGFVE
                     RYTLVGVGEEDYDLDRIPVNSPLAKGLLGKRVGEIAEVDVPAGLRRLEIVELAYDDES
                     VMGG"
     gene            complement(1582..4320)
                     /locus_tag="PLD05_07115"
     CDS             complement(1582..4320)
                     /locus_tag="PLD05_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP77243.1"
                     /translation="MLRAVAGIVCIGLLTMGQMTQGWAKETVLYVAPNGNDSWSGRIS
                     QPNPAGNDGPLATLTGARNAIRRLKGEGQTGPFRVLIASGRYSLKEPLVLEPQDSGTS
                     ESPIVYEAASKEKPRFSGGRQITGWRKYDDRLWVTTVPDVADGKWYFEQLWVNGRRAT
                     RARSPNKFYYYVQKKIDVYQDADSGKIVETVNRAFQARPEDIKPLLTLTRQQLNDVTL
                     VTFQSWETSRSRVESIEPQQSIVTVTAPIPWGFAYWGPNCRYHLENFRAALDEPGEWF
                     LERDGTLYYYPYPDEDMQKAEVIAPEVSEFIVIAGRPEEQKLVQDVTFRGLIFEHSQY
                     VLPPEGHADSQAEVTIPAVIMLDGAKNVTFEDCEIKHVGIYGVWFRRGCQECRIERCW
                     LDDLGGGGIKIGEGRGADLSRAEVHTKKIVCRNNIIHAGGRIHHGAIGVWIGHSGYNE
                     VVHNDISDFFYTGVSVGWVWGYKESLAHHNRIEYNHIHHLGWGVLSDMGGVYTLGVSP
                     GTSVSHNVVHDIYSYDRYGRGGWGLYNDEGSSHIVLRNNLVYRTKTGGYHQHYGRENR
                     VENNIFALSMEGQIQRSRVEDHISFFFERNIVYWKGGPLIVAGQVNDDNVRFTSNIYY
                     NAAGEPIDFQGLTLEQRQAKGWDLGSLIIDPRFIDPEKGDFRLAEDSPAKRVGFVPFD
                     YSLAGLEGDEAWREIPKRFVYPEVEFAPPPPPAPPLTMEESFELMPVGHPPRDGQVNT
                     ERKGDAILVVNERASDGRRALVFRDAEGLAHAFNPHWVINPNHTSGKTSLQFDIWMGS
                     GAILFHEWRTWDTGPYRVGPSLWIQNGQLTVAGKKLMDIPLEQWVRIEVSCAVGTSSD
                     GTWTLRVTLPDGSSKVFDSLPLGNKEFRDLTWIGWCSMAQQESVFYLDQVKLERQP"
     gene            complement(4399..5853)
                     /locus_tag="PLD05_07120"
     CDS             complement(4399..5853)
                     /locus_tag="PLD05_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77244.1"
                     /translation="MRTTLHCCFAIVLSAIIAGRPIYGGNVAEKPNFVVINIDDLGYA
                     DIGPFGSKINRTPNLDRMAAEGRKLTSFYAAPVCSPSRAALMTGSYAKRALPIPHVLF
                     PANDVGLSPDEVTLAEVLKTAGYTTALIGKWHLGDQPPFLPCQQGFDYYFGLPYSNDM
                     GPAEDGVKSSLGEPLPQRKPGQVGQPPLPLMRNNTVIKRVLPDDQQALVEMYTEEAVG
                     FIKRNADKPFFLYLAHNAVHFPIYPGKKWAGRSPHGIYSDWVEEVDWSVGQVLDTIRS
                     LGLSEKTLVLFTSDNGGTQRAVNRPLRGFKGSTWEGGVRVPTIVWWPGKIPAGTECDA
                     IMGMFDVLPTFAYLAGAPLPQDRKLDGMNVWDHLTGAAGDRPAHEVFYYYRGLKLEAV
                     RYLQWKYELPGVRGKEQKAKLYDLKADIGETTDVAASHPEVVKKIELLIQAAESDLGL
                     DSMGPGCRPLGRVEDAQPLIGYDGKVRPGFEPKE"
     gene            6190..6789
                     /locus_tag="PLD05_07125"
     CDS             6190..6789
                     /locus_tag="PLD05_07125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOP77245.1"
                     /translation="MKRTIILASQSPRRRQLLQEAGYSFVVIPPDELAECGICSGESP
                     PQFAARLAYQKAANVASRIQEGLILACDTVVSCDGQILGKPRDEDDARKMLHLLRGRE
                     HSVFTGVCLWDYPESSPKIQVAETRLVMAELTDEEIEEYLASGLWEGKAGAFGYQDRL
                     GWLRIIEGSESNVVGLPLECLERMLREYQQPRPTDSMHL"
     gene            complement(7078..7404)
                     /locus_tag="PLD05_07130"
     CDS             complement(7078..7404)
                     /locus_tag="PLD05_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOP77246.1"
                     /translation="MAKDTSAKKPLTKTQLRAAIAEATGLKKADVAAVLDALTKEIEK
                     ALSPRGPGVFILPGLLKIKKAKTKPTPARQMPKPGKPDEIITIPAKPARNVVRVRALK
                     QLKAMV"
     gene            complement(7696..8835)
                     /locus_tag="PLD05_07135"
     CDS             complement(7696..8835)
                     /locus_tag="PLD05_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HOP77247.1"
                     /translation="MDEASIGAKNVTRFGDYSYSDIATSPLMLYYEVTQACDLVCKHC
                     RASAQSQPHPEELTTELAKSLIDQVTSFPKVPNMVFTGGDPLKRPDLFHLLEYAVNRG
                     INVALTPSATPLATPEALRRARAVGVSALGLSLDAADAPTHDAFRGWEGSFARTLEIL
                     AVARELGFYIQVNTTISKRNVDQIDSMADLLRNLGIHMWSVFFLVPVGRGALEERISP
                     EEYEHVFGRLWSHAQHQPYAIKTTEAPHYRRFVLEVLRQKDKAHPDGDRTQPMAKRLT
                     MGIGDGKGIMFVSHIGEIYPAGFLPLACGRFPNASVVSVYQNHPVFRALRNPDALKGK
                     CGTCAYRIICGGSRARAYAVTGDYLAAEPDCVFQGEPGTPVVHPS"
     gene            complement(8976..10286)
                     /locus_tag="PLD05_07140"
     CDS             complement(8976..10286)
                     /locus_tag="PLD05_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77248.1"
                     /translation="MMNRLMRLVTALMLLVCGWGTRFALGQERQKVVIPFDFESRFDG
                     GRYGQMVGDMIWQKLHREGGFVIPDSMADVRDYCSMNQIKLGAETPLEDVRKIVKRDF
                     DADIGIWGSIERAPGASGEIYDITIKCVDFSSSAEPKVIYEKSARTNSVSEIPHLYVQ
                     EMLNALYEREPGRVAAVNPIAEENWQKNPNLILGGDFERAIRGVPVGWEDRAGQDREP
                     LGRLVAWVPEPENPNNHVIRFTFDQGVGDSTGVMYYSLPFQVQEGATYRFQCRWRSDG
                     PAVKVFIKCYAEMPSEYRVQGTQPGPGSPSSSTRGGATGTGSPSARSGRTTAPGYVDW
                     SQMRECYRSQQNLKGPLNTWNVHTEDFTPRHTQYTPTWGRVMLYAYLGAGKVEFDDVV
                     VKMIMPASPGDSKKDPRRSMESSVTIKEMEERERASRSGSSGGK"
     gene            complement(10304..13660)
                     /locus_tag="PLD05_07145"
     CDS             complement(10304..13660)
                     /locus_tag="PLD05_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77249.1"
                     /translation="MMSPAAEGVIRRLDATRQQWWFFTLLTSCVMAVSVSLATLLILV
                     LADALLRLSQPVLLGAFLVWMAVNLGLLVWVVRRVARSHRSLEATARRIEDQFPELQN
                     ALINVLQLLNDPRQCNPAFCLAAVNHAASQASRIRFEEAAARHTRWERFCYAIHTSRD
                     LLEWTGILVSLILLSVLCSLMLPNWGSAATRLMQPWHFVPVVGRVQILEVSPGNTEVL
                     VGSNVTISAKVRVPEKKPYDAFLYVIEDGKKEERFPMAVDKDLENYSFSLPSVLKPVK
                     YRVEVGDSQSPLYRLGVSEIPSIAEVEVTLDYPPYMEKPRDTFRQRVADLRAPQFSEA
                     TLKIIPSVPIAGGEITLDGQPLIGTTEDGGRAFLVKFPLLRDGEFAVKLRNMAGLTER
                     APRINRITVIPDQPPTVEIVKPTSQSSAAVGDKVPIRVRASDDYGLGRIRLEMKIQQA
                     DADGAKGSSDETEPLKLQDWDNPPGPFVDYELVLSPERFQPGQVVLLRAVAWDKRNLG
                     KFGLDLRPQKAESPWVMIRLISTETKTTEELKTLENLRAAIFRILEEQIRAKGALVTL
                     TRTIEDNPGQFSGGIHAIRARQVEIRSSTEAVVKSILDTDSEERKIIRRVLSNLVANE
                     MLQAVQECDRLVKLSDLSKAKDEMVPLGQHQDQIIETLRKILDAARIAQTELLKEAEK
                     RAGGDLPDDVKKKLEEIHSKLQEFLEQQKKVIEASEELSKKPVEDFTEKEEQILKELA
                     ETQDDWSRFMKEAHTDLSKLPEQDFANATLCQELNEIQTELKMAEDALLKKSVDIAVP
                     LEQLGYEMAKELTTNLEKWLPDTPDRERWSQEESLSDQDKEAPMAELPGQLEDLIGEL
                     MEQEEDLFDEMEDISSSAADSLDKGAGWDAADGPISNMSAKGVTGNRLPNTSEIGGRS
                     GEGRSGKSSGEFVSEEAVGKGGRKTPSRLTPDPYMKGQIKDFSKDPTGGATGGGKESG
                     QGGQGLEGPTPPPHGPRELQRLAGKQAELRNKAESINAQMQVLNYHHTDMEQLIELMK
                     EIERDLLAGRYQNALRKREVVLQGLRTQKEYVGGEFEVKQDATLNIPKDVQKEILGSV
                     QEPAPAGWEEIVRGYYRQLIGTSTGTGRPQ"
     gene            14167..14823
                     /locus_tag="PLD05_07150"
     CDS             14167..14823
                     /locus_tag="PLD05_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77250.1"
                     /translation="MAAVIAVGFLWFGDLATASEPAAINPFGPPVSEREDAVPGYVEL
                     SDGKIIAGKVYMTRDKRVKVFDSTLQRQREIPLNRIKQIECIVVSERMEKEWRFRETT
                     SNVKEYTGRSYPTREYLHRITLDDGRTIEGPLSEVIYVQPEPSGSSSRGQQAAIEPQR
                     FILYKTHKGPVGSDLKSLVYVKVVKLGEEALAEGQKKGSHSTSSRTTSQEASESKSRP
                     "
     gene            14877..17123
                     /locus_tag="PLD05_07155"
     CDS             14877..17123
                     /locus_tag="PLD05_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOP77251.1"
                     /translation="MVEISLANGASIVSLVLVLAVAAGLVTVFYYRKFGELKRGRWQL
                     LLLLRLLAVVIVVLLLFRPVVHFYREERQKPTVVFLLDTSASMKIADDVSGIPRFTQA
                     RDKILLWAERLKEHFALQVIPFAERAGETIAISQLGTLSPDGPATSLSRALVAATKAA
                     PRSEMEAIFLVSDGIHNSARKPEEVSSRMQVPVFTIGVGTSLKTDASFKDIQITGLDC
                     PSRMMLNNLTRIKAFVEAVGLAGRVVKVVFLEDGQTIQEQELVLDDQPGPQEVMFEYR
                     PPVKGRHVYAVRIDPLSEEKIPENNERSAPALVVEAQIKVLYLEGTLRAEYGAIVDRF
                     LSKDPNIEFYALVQVKKNTFVKRTNMEGLTLESIPSDEATLNQFDVFIIGDLDSSFLS
                     SAQQEAIVNRIKNGGGLIMLGGYHSLGPGGYANTPIGQILPVELGDRDIGQLTDPFLP
                     ELTPDGRQHPIFANIARFFPTGGSSAEQQQLPPLDGCTRVGPARAAATVLAICPLTGE
                     RMPVLAVMPVENGRTAVFTGDTTRKWQQVPRVLDQESPFLQFWGQFVRWAAGRSEQLE
                     AKASVTAATDKAAYEPDEPIQLSAVVRDQRGEGTDKAKVTATIRAPSGAFDKVALVST
                     PGPGGHYSASYEPRFHGTLEIEVQAELEDEKLTAEKLIVEVGRPYLEFEKLDLDEKTL
                     TQIAADTGGRYAHISAADQLIDQLNQAERKKRIYVEQPLFWPPLFWAIFVALVTTEWV
                     LRRRFQLR"
     gene            17120..18019
                     /locus_tag="PLD05_07160"
     CDS             17120..18019
                     /locus_tag="PLD05_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP77252.1"
                     /translation="MSPQSAYRYVEPSALARVKNLQLVARGVVEGFISGLHASPYKGF
                     SVEFAEHREYVPGDDPRHLDYRMFARSERLYVKQYEEETNMRVQILLDVSGSMNYRYE
                     AKITKLEYGCYLTAILAYMMTRQQDSVGLTAFDDTIRLDMPARSSPRHFNEMMRQLEN
                     LQPGKTTGLAQVLHKLANRFKKRCLIILISDLYDDVDKVIRALHHFRHRKHEVIVFHV
                     FDKAELDFPFDDLVVFQDLETDEKIQVDPLYVREEYQKLLSEFVAEYRRACLESSIDY
                     VMTDTSVPYDFMLSRYLTKRNQL"
     gene            18016..20262
                     /locus_tag="PLD05_07165"
     CDS             18016..20262
                     /locus_tag="PLD05_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOP77253.1"
                     /translation="MTFGAAAFLLAALAAIIPPILHMINRQRAKQLPFSTLRFLKISV
                     QKTRRRRQIRDLFLMLVRMAVLIFIAIGLARPTLSKLRNFMGGGQSAIAIVLDNSASM
                     GWIDEGRPRFEWAINAANQILNELQGGDEVTLWVTSGPRYPELGQVDRTHEKVRQLLG
                     QIAQRGPTFERGELGHLVREARYQLLQSKAANRYVFVITDNQAVAWEDLSMDEGTAEK
                     TADQEVEKSKDPHLRQIPIIIVDCQRNPKPNVAIRNVELSTILPVAGIPMKVIIELFN
                     SSSIPQQRVAELYMNGTKVGSSPEVSLAPQSTAVTEIVFTLDRGGTHRAEVRLSGEDG
                     ARYDDRRFLSLEVDPAIPVAIVTSERHEIPYLNDSFYLEKALQPVTDESWALKPSILT
                     AAALTSDPLSNYRIIYLVDLPEIDKPMAARLVEFLQSGGKLVWICGQDTQVASYNAAN
                     EAVNGQLLPAALDSVMAASTEENRDSWQIAWIDVQHPALVGLNDPPSLHQSVLVYKFI
                     KTHSPVGSGARVLARLDGAGDPLILERAVGQGTVVWVGTSVHVDWTNLPLRPFFVPFM
                     TRLAFYLAGTEPERLKVLAGQPIVIPYQGQIPPSVVEVVPPDGALLRLNLERGQTPEF
                     RYVDTHAVGFYQFRLLASVRPIQKLYAVNPDPDESDPEKISREQLEEAFSETTVLFAD
                     NPEDLAPTFALLRHGRSLSELFLTMVLIGLVFETFISNWFSPKEQDQQPKLLSQYRPR
                     QRLSRTVG"
     gene            complement(20335..22059)
                     /locus_tag="PLD05_07170"
     CDS             complement(20335..22059)
                     /locus_tag="PLD05_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOP77254.1"
                     /translation="MKLSYLSLAMLLSFVGLSANAAQAQPVVWIETSTLRICRSETPP
                     PSPSREVNLTAARNEWVSFQVFLRSSHPLPAVGVELSPLEGQSGGNIDSACFRIYREH
                     QLELKEPTYRNDAFRADWYPDPLIPARHPLTGEPLSGPRFTAQPFDLPANETHGFWVD
                     VFVSKDVPPGTYRGTLKVMSQEGQVVAMPVNLEVWDFTLPRVPSLVTALGSPAERMRA
                     YYARQSKEGKESEQVDWATVEKQCALLAQEHRINAYPPSDWLRLEREGEEFRLSDENV
                     ARLREWIDTYCVNAIQVPSPVGIVKDPQANNQALQAWARAWDRAIESIDRPHVVYYIY
                     LKDEPNDPEAYEFVRVWGKAIRATKTKVKVMVVEQTRTQNPAWGDLYGAVDIWCSLFP
                     LYDEPTARERQALGEIMWAYTALCQLAKTPWWHIDYPLLNYRVPCWMSYRYDIRGLLY
                     WGGMSHWSQVEDPWTDPRTYRPGRSNRPLIYNGEGTLVYPAKPCGYDGIVPSLRLKAL
                     RDGIEDYDYFMILQRAGQGEKALKIVQQLAPSWFEWNPDPQAYEAARRELAKAILSLP
                     NEVRNPTW"
     gene            complement(22452..23477)
                     /locus_tag="PLD05_07175"
     CDS             complement(22452..23477)
                     /locus_tag="PLD05_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77255.1"
                     /translation="MMLEPGDAHDSSVPLPDGGRRAVRSPRTFERSPTTFARSRESLS
                     RHPLDAALAFLRWRFGSEFLRSSAHLFLICGKYGLLVGAVLVVFQCLQKAGGQAWAIL
                     IGISLALLILVFHYIGGRFLFVLERWDRQINGYLSSSVVPDALSLLSLVGSSAMLVVG
                     AIDALITGSFELVFWGVAVFIWGLFVALQSLNLEGMNLQVSPELSPADEILGLIQFGL
                     KILVRLTPVVFGVMVLVGNLQLVMEIIIDGPELNRPEAYAAVGQQASDGKLVQASLDV
                     REPPFRVLRGRETLVMAGVLPGAMYLIYVLGHFASAIFSVFLAAVPENARGGMMTDHP
                     GKNDSTG"
BASE COUNT         5502 a         6859 c         5997 g         5430 t
ORIGIN      
        1 cctccgttgc caaagtcctt ccagccacgc caccggcccg gcacataagc aggatgatat
       61 ggtcgatagg gggcaggccc aagccaaagg tcccaatcca aatagggagg aactggtggc
      121 gtatctttgg gccgatcgcc tccgctgtag ctcgccgcac tccacacgtg aacttccgaa
      181 atcgtcccga ttacgttagc cttgaccaat tcgaccacgc ggcgatagtt gtccccagcg
      241 tggatctgcg ttcccaattg cgtcaccaat ttatttcgga tggccaactc cgtcaaatac
      301 cgcgtctcat agacacaatg cgtaagcggc ttttcacaat aacagtcctt gccaagttgc
      361 atcgccgcag cagccgccgg ggcgtgtgtg tgatctggcg tgctcaccac cacggcgtcg
      421 atctctttag ctatttcatc gaacatgcga cgaaagtcac ggtataactt ggcgttcgga
      481 aagtctttcg ctgcttcgtt gagattattg gcgtccacat cacacagcgc cacaatgttt
      541 tcgcttgcaa caccggcgag attcgctgcc ccacggcctc gcacaccgat cgctgcgatg
      601 ttgagtttct cattaggaga ttcagcaaaa ctcggcacaa ttgtggccgc aaacggggcg
      661 ctagccaagg ccgccgaaat cttcagaaag cgtcttctcg gggaacgcgt gaacatagct
      721 tagctccata aaaaacgggc agtctgccaa agttcccagc tatgaggcag tttataggat
      781 gtgttccact aaccgaattg ggtgtcccta tcttaggaaa acaagcggca gaactcaagc
      841 agcagaggaa ggaccctctt ggacaaaccc ttgccatctc ggcagaagca cctgtcgcaa
      901 gacatcgccg ccccatttta gccgcccata accgattcat catcgtaggc tagctccacg
      961 atctccaagc ggcgaagacc ggccgggacg tcaacctcgg cgatctcccc cacccgtttc
     1021 ccaagcaaac ccttggccag cgggctgttg acgggaattc gatccaggtc gtagtcttcc
     1081 tcgccaacac ccaccagggt atatcgctca acgaaacctt tatccaaatc gcggacagtt
     1141 acagtacatc ccaaggcaac acggtctcgc ggtatccgag aaggatcaac aatttcgcac
     1201 cgggcaatct tgtcgcggag ataattgatc ttcgccatca caagcccttg tgtctcccgc
     1261 gcgccgtgat attcggcgtt ctcccgcaga tcgccctcct ctcttgccgc ggccacacgc
     1321 ttcgtcacct cggcaagctg atcctccaac tctctcagct ctcgacaaag cttttcgtaa
     1381 cccgcctgtg ttacgggaat ccgttcatcc atccgatttc tctccctcaa agactccagg
     1441 atactctctc ccaaaccaaa gcttcactat gacactgacc gttcaaaata atcctgcatg
     1501 gtaggacatc acctcgtcct caccctcctc atacctggtt gcacgcctac cgctcgtccg
     1561 cgaataatcc ctgctgtttt gtcacggttg acgttccagc ttgacctgat ccagatagaa
     1621 gaccgattct tgctgggcca tgctgcacca gccgatccac gtcagatcgc gaaactcctt
     1681 gttacccaaa ggcaaactat caaacacttt gctgcttccg tcaggaagtg tgacgcgaag
     1741 cgtccaggtt ccatcgctcg atgtgcccac tgcgcacgat acctcaatcc gcacccactg
     1801 ctccagggga atatccatca gctttttgcc cgccacggtc aattgaccat tttgaatcca
     1861 aagggacggt ccaactcgat aaggccctgt gtcccaggtt cgccattcat gaaagaggat
     1921 ggcaccgctt cccatccaaa tgtcaaactg taacgatgtt tttccgctcg tgtgatttgg
     1981 attgatcacc caatggggat tgaaagcatg ggccaaaccc tcggcgtcgc ggaaaactaa
     2041 ggctctacgc ccatctgagg cccgctcatt aaccaccagg atggcatcgc ccttacgctc
     2101 ggtgttcact tggccatctc gcggtggatg ccccaccggc atcagctcga acgattcttc
     2161 catcgtcaag ggcggggctg gcggtggggg tggggcaaac tccacttctg gataaacgaa
     2221 ccgcttcggt atttcgcgcc atgcctcatc gccctcaagt cccgctaaag aataatcaaa
     2281 aggcacaaac ccaacccgct ttgccgggga atcctcagcc aggcgaaaat cgcccttttc
     2341 tgggtcgatg aaccgcggat caatgatcaa tgacccaagg tcccagccct tggcttgtcg
     2401 ctgctccaga gttagccctt gaaagtcgat cggctctccc gccgcgttgt aatagatgtt
     2461 tgaggtaaag cggacattgt catcattaac ctgaccagcc acgatcaaag gcccgccttt
     2521 ccaatacaca atgttgcgtt cgaaaaagaa ggaaatatgg tcctccaccc gtgatcgctg
     2581 aatttggccc tccatgctca gggcgaagat attgttctcc acgcggtttt cacggccgta
     2641 atgttggtga taaccacctg tcttggtgcg atacactagg ttatttctca ggacgatgtg
     2701 cgagcttccc tcgtcgttat aaagtcccca gccgccgcga ccgtagcgat cgtaggagta
     2761 aatgtcatgg acaacgttat ggctgacact tgtcccgggc gaaaccccga gcgtgtaaac
     2821 acctcccata tcacttaaaa caccccatcc caagtgatga atgtggttgt actcgattcg
     2881 gttgtgatgg gccagcgatt ccttgtagcc ccagacccaa cctactgaaa cgcccgtgta
     2941 aaagaaatca ctaatgtcgt tgtgaaccac ctcgttataa ccgctgtggc cgatccaaac
     3001 accgatcgcc ccgtgatgaa tccgcccacc cgcatgaatg atattgtttc gacaaacgat
     3061 tttctttgta tggacctccg cacgactgag gtctgcgccc cggccttcac caatcttgat
     3121 gccgcccccg cccaggtcat caagccaaca cctttcgatg cggcactctt gacaaccccg
     3181 tcggaaccac acaccgtaga ttcctacgtg cttgatctcg caatcctcaa aagtgacatt
     3241 cttggcccca tccagcatga tgaccgccgg aatggtaacc tccgcctggg agtcggcatg
     3301 tccttccggg ggcaacacat actggctgtg ctcaaagatc agacctcgaa aagtaacatc
     3361 ttggaccagc ttctgttcct cgggacgccc tgcaatgacg ataaactcgc ttacctccgg
     3421 ggctatcacc tcggccttct gcatgtcctc atcaggatag gggtaatagt agagtgtccc
     3481 atcgcgttcc aggaaccatt cgccgggttc atcgagggca gcccgaaagt tctcgagatg
     3541 ataccgacaa ttgggtcccc aatacgcaaa tccccacggg atcggcgcgg ttactgtgac
     3601 aattgactgc tgtggctcaa tcgattcaac ccgcgaacga gacgtctccc aggattggaa
     3661 cgtcaccaac gtgacatcat taagttgttg acgagtgagg gtcaaaagtg gcttgatatc
     3721 ttccggtctt gcctgaaacg cccggttaac ggtttcaaca atcttacctg aatcggcgtc
     3781 ctgatagaca tcgatctttt tttgcacata gtaatagaac ttgttggggc ttcgagcacg
     3841 ggtcgctcgt ctgccgttaa cccaaagttg ctcaaagtac catttgccgt cagccacatc
     3901 ggggacggtc gtgacccaaa gcctgtcgtc atatttcctc caacccgtta tctgcctgcc
     3961 tcctgaaaac ctcggctttt ccttgcttgc cgcctcatag acgatgggcg actcgctcgt
     4021 gccagaatct tggggctcaa gaaccaacgg ttcctttagg gagtaccgac ccgaagcaat
     4081 caagacacga aatggacccg tttgcccttc tcctttcaaa cgacggatcg cgtttctcgc
     4141 gccggtcaaa gtcgccaacg gaccatcgtt acctgctgga ttgggctgac ttatccgccc
     4201 cgaccaggaa tcgtttccgt tgggcgcaac atatagtact gtttctttgg cccatccctg
     4261 ggtcatttga cccattgtca acaggccaat gcacacaatt ccagccacag cccgtaacat
     4321 ggcacgtgtt cctccaactc cgcgatgtca aacagtcttg cacaactgcg ggatccatct
     4381 gccggttggc gcagatgctc attccttggg ctcgaaaccg ggccgaactt ttccatcgta
     4441 accgatcaaa ggctgggcat cttccacccg cccaagagga cggcagccgg gtcccatgct
     4501 atcaaggcca aggtcgctct cggcggcttg aatcagcaat tcaatcttct taacaacttc
     4561 cggatgcgac gcggcaacgt ccgtggtttc gccaatatcg gccttcaggt cataaagctt
     4621 cgccttttgc tccttgcccc gtacccctgg tagctcatat ttccattgca agtagcgaac
     4681 ggcctccagc ttcaaacctc ggtagtagta aaacacctca tgggcgggac gatcaccggc
     4741 agcgcccgtc aagtggtccc acacgttcat gccgtcgagc tttcgatcct ggggaagagg
     4801 ggcaccagcc aggtacgcga acgtaggcaa aacgtcgaac atccccatga tcgcatcaca
     4861 ttcggttccc gcaggaattt tgcctggcca ccacacgatg gtgggaacac gaacgccacc
     4921 ttcccacgtg ctgcccttga accctcttaa cggtctgttc accgctcgct gtgtcccgcc
     4981 gttgtcactt gtaaacagca ccaaagtctt ttcgctcagc ccaagcgagc gaatcgtgtc
     5041 gagcacctgg cccacactcc aatcgacctc ttccacccag tcggaataaa tcccatgcgg
     5101 ggaccgtccg gcccatttct tcccgggata aatgggaaaa tgcaccgcat tgtgggccaa
     5161 gtaaaggaaa aacggcttgt ccgcattcct cttgataaaa ccgactgctt cctcagtgta
     5221 catctcaact agggcctgtt ggtcatccgg caggacccgt ttgatgaccg tattattgcg
     5281 catcagcggc aatggcggct gacctacttg ccccggtttc ctttgaggaa gcggttctcc
     5341 caaactactc ttgacaccat cttcagcagg gcccatatcg tttgagtaag gcaaaccgaa
     5401 gtaataatcg aaaccttgtt gacacggcaa aaatggtggc tggtcaccga gatgccattt
     5461 gccgatcagg gccgttgtgt atcccgctgt tttcaagact tccgccaacg ttacctcatc
     5521 gggagacagt cctacgtcgt tggcaggaaa caagacatgc ggaatcggta gtgcccgctt
     5581 ggcataactc cccgtcatga gcgcagcacg tgaaggagag cacacgggcg cagcgtaaaa
     5641 cgaagtcaat ttgcgtcctt ccgccgccat ccgatcgagg ttcggcgttc gattgatttt
     5701 tgatccaaac ggaccaatgt cagcataccc caggtcgtca atgttgatca cgacgaagtt
     5761 gggtttctcc gcgacattgc ccccgtaaat cggccttcct gcgataatcg cacttaacac
     5821 aatggcaaag caacaatgca gggtagttct catcctgttg tcctccccaa aaggtactaa
     5881 agatcaaaac cagaaactgt ttccgagtga caaccgccac ggcgcggccg atcatggaaa
     5941 cggtcactac gcagggtacc caagttggcg agatggtttt tcggaaaaag tacatcccgt
     6001 ttggtgtatt acattgtcgc caaggtatag gacgttagca acggcccggg gtggtgccat
     6061 gaaggcttgt cagtctctcg aggatgctag ccggcccttc aaacgtggca tactattgta
     6121 aattgaagag ttatcgagta agaggctgtc ttcaccactt actaccagtt aaaaggcatg
     6181 acgtctccct tgaaacggac cattatcctc gcaagtcaat cacctcggcg gcgtcagttg
     6241 ctgcaggagg cgggttattc ttttgtggtg atacctccgg atgaattggc tgagtgcggg
     6301 atttgtagcg gcgaaagtcc gccacaattc gccgcacggt tggcctatca gaaggccgca
     6361 aatgttgctt ctcgcatcca ggagggactg atccttgctt gtgacaccgt cgtgagttgt
     6421 gacgggcaaa tcctgggtaa accgcgagat gaagatgacg cgcgcaagat gcttcatttg
     6481 cttcgaggaa gggaacattc ggtctttact ggtgtttgcc tgtgggatta cccagaatct
     6541 tcacctaaga tccaagtcgc tgaaacacgg ctcgtcatgg ccgagttaac cgacgaggag
     6601 attgaggagt acctggcttc cggcctttgg gaaggcaaag cgggggcctt cggctatcaa
     6661 gatcgcttgg gctggctgag gattatcgaa ggcagcgaaa gtaatgtcgt tggcctacca
     6721 ctggagtgtt tagaaaggat gttacgagaa taccaacagc cccggccaac cgattcgatg
     6781 cacctatagc gccgttggcc aaaccgggaa attattgcaa taaaacctag ccgctcaaat
     6841 cgcttagctg gaatacgcac taagccggtc cgcttacgag gcggtatccc atctccacga
     6901 aaagctcttc ggtcagtaga tacgccaccg aggccgcgac aaccaaatct cctcaattgg
     6961 atctggtcga tctagcggtg gtccaaacaa cccctaggca cggtacactc ctcgccccac
     7021 gtgtttgcta gtcgccagca cccggtggcg tttcaacgac cgaattcccc agacgaatca
     7081 caccatcgcc ttgagttgct tcagcgcacg aacccgaacg acattccggg ctggcttggc
     7141 cggaatggtg atgatctcgt cgggtttacc aggttttggc atctgacgag ccggtgtcgg
     7201 cttggtcttt gccttcttga tcttcaagag accagggaga ataaacacgc cgggccctcg
     7261 ggggctgagc gccttttcga tttccttggt cagcgcatcc aacactgcag ccacgtctgc
     7321 tttcttcaag ccggtagcct cggcaatcgc ggcacgcaac tgtgttttcg tcagcggttt
     7381 ctttgcactc gtgtcctttg ccatagaccg cgtacctcca aaacatgtgt tcaggaaacg
     7441 tgaaacttgt ccgtccgaaa cgacattttt cgggcgagga cagcaccgtg cttgtcatga
     7501 ttagaacgat tttccgagaa atttcaagcc cacaacccca agaaaaaccg tcgaaaaggc
     7561 ggattttttg agaatttgcc cacttccaat cggcgattgg atcttacatc ctacgggaag
     7621 tcccccttgt cctcgcccaa caaacgtccc tatcgctgtt ccctcactac gagtgtcaag
     7681 aaaaagcacc tcatttcatg aaggatggac cacgggcgta cctggctcgc cctggaagac
     7741 acaatccggc tctgccgcca gatagtcacc cgtcacggca tatgcccggg cacggcttcc
     7801 accacaaatg atccgatacg cacatgtgcc gcactttcct ttaagggcat caggattacg
     7861 tagggcccga aaaacggggt gattttgata caccgagacg accgacgcat tcgggaatcg
     7921 cccacatgcc aggggcaaaa agccggccgg atagatctca ccaatgtggc tgacaaacat
     7981 aattcccttg ccatccccaa ttcccatggt gagccgtttt gccatgggtt gggtgcgatc
     8041 cccatcagga tgagctttgt ctttttgtcg taacacttct aacacaaatc gacgataatg
     8101 gggggcctcg gtggtcttga tggcgtaagg ctgatgctgg gcgtgagacc ataatcgtcc
     8161 aaaaacgtgt tcatactcct ccggggagat tcgttcctcg agcgcgccgc gccccaccgg
     8221 taccaagaag aagaccgacc acatgtggat acccaggttc cgcagtaggt cagccatcga
     8281 gtcaatttga tccacattcc gcttgctgat agtggtattc acttggatgt aaaagcccaa
     8341 ctcgcgggcc acggcaagga tctcaagcgt gcgtgcgaaa ctgccctccc agccacgaaa
     8401 ggcatcatgc gtcggggcgt ccgctgcatc aagactcaac cccaaggcgg aaacgcccac
     8461 agcacgggcc cgcctaaggg cttctggggt ggccaagggt gtcgcagacg gcgtgagcgc
     8521 gacgttaatc ccccgattga cggcatattc caagaggtga aataaatcag gccttttcaa
     8581 gggatcaccg cccgtgaaaa ccatgttggg cactttggga aaagacgtca cttgatcaat
     8641 gagcgatttt gccaactcgg tcgtcagctc ctcaggatgc ggctgcgact gagccgacgc
     8701 tcgacagtgc ttgcaaacga ggtcacatgc ttgcgtgacc tcgtagtaga gcataagcgg
     8761 actggtggca atatccgagt agctgtagtc gccaaagcgc gttacgtttt tggccccaat
     8821 tgacgcctca tccatccgtc agtccttccc aattaagagg ctggaaacat gggttcctgt
     8881 ctcaaaaaac tttaggtgac aggtctgctt gtaaattata gagtgcgaga cgagttaaac
     8941 tatgccattg acagagaggg gccggttctg cagtatcact ttccgccact tgatcccgac
     9001 cgggacgcac gttctcgttc ttccatttct ttgatcgtca cagagctttc catggagcga
     9061 cgcgggtcct ttttcgagtc ccctggagag gcaggcatga tcatcttgac aacgacatca
     9121 tcgaattcca ctttgccggc cccaaggtaa gcatagagca tcactcgccc ccacgtcggc
     9181 gtgtactggg tatgtcgtgg ggtgaagtcc tccgtatgca cattccaagt attaaggggc
     9241 cctttgagat tctgctggct gcggtagcat tctcgcatct ggctccagtc cacatatccg
     9301 ggggcagtcg tccttccact ccgtgctgaa ggactcccgg tccccgtggc acctcctctt
     9361 gtagaagagc tcggcgatcc aggaccgggt tgtgtcccct ggacgcgata ctcacttggc
     9421 atctccgcgt aacatttgat aaatacctta acggccgggc catcacttct ccagcgacac
     9481 tggaaacgat acgtcgcccc ttcctggacc tggaacggaa ggctgtagta cataacgcca
     9541 gttgaatcac caaccccttg atcaaaggtg aaacgaatca cgtgattgtt cggattctct
     9601 ggttctggga cccacgcaac gaggcgaccg agcggctcgc gatcttgccc agcacggtct
     9661 tcccatccca cgggaacccc acgaattgcc ctttcaaagt ctccgcctaa tataaggttt
     9721 ggattctttt gccagttttc ctctgctatg gggttcacgg ctgcaacacg ccctggctct
     9781 cgttcatata gggcgtttaa catctcttgc acgtataggt ggggaatctc gctgaccgag
     9841 ttcgtcctgg ccgatttttc gtaaattacc ttcggctcgg cactactgga aaaatccacg
     9901 cacttgatcg taatgtcgta gatttctcca gaagcgccag gggctcgctc gatcgaaccc
     9961 caaatgccaa tatcggcatc aaagtctctt ttcacgatct tgcggacatc ttcaagaggc
    10021 gtttctgcac ccaatttgat ttggttcatc gagcagtaat cccgaacatc ggccatcgaa
    10081 tccgggatga caaagccacc ctcccggtgg agcttttgcc aaatcatgtc cccgaccatt
    10141 tgaccgtagc ggcccccgtc gaaccgggac tcgaagtcaa agggaataac caccttctgc
    10201 cgttcttggc ccaaggcaaa ccgcgtgccc cagccgcaaa caagtagcat gagtgcggta
    10261 actagtcgca tcaatcggtt catcatctca gacgctgtct ccctcactgc ggacgaccgg
    10321 taccagtgct cgtgccgatc agttgtcggt agtatccgcg gacaatttcc tcccacccgg
    10381 ccggggccgg ttcttgaaca cttcccagga tttccttctg gacatccttg gggatattca
    10441 aagtcgcatc ttgtttcacc tcgaattcac cgcccacgta ctccttttgg gtccgcaatc
    10501 cctgcaagac gacctcgcgc ttacggaggg cattttggta tcgtccggcc aacagatccc
    10561 gctcaatttc cttcattagc tcgatcagtt gctccatatc cgtgtgatgg tagttgagga
    10621 cctgcatttg ggcgttgata gattctgcct tattgcgaag ctcagcctgc ttgccggcca
    10681 gtcgttgcaa ctccctcgga ccatggggcg gcggtgttgg accctcgagt ccctgcccgc
    10741 cttggccact ttcctttccc ccgcccgttg cgccacctgt cggatcctta ctgaaatcct
    10801 taatttgccc cttcatatag ggatcagggg tcaagcgact tggcgttttc cgacctcctt
    10861 tgcccacagc ctcctcgctg acgaactcac cgctcgattt gccgctgcga ccctcacccg
    10921 aacgtccacc gatttcactt gtgttgggta atcggttgcc cgtgacgcct ttcgcgctca
    10981 tattcgagat cggaccatct gcagcatccc agcccgcccc tttgtctaac gagtccgcag
    11041 cacttgacga gatatcctcc atctcgtcaa agaggtcttc ctcctgttcc attagttcgc
    11101 cgatcaaatc ttcgagctga ccaggaagtt ccgccatcgg tgcttcctta tcctggtcac
    11161 tcaatgactc ttcttgactc cacctctccc gatcgggcgt atcaggcaac cacttttcca
    11221 agttagtcgt cagctcttta gccatctcgt agccgagctg ttcaagcgga acagcgatgt
    11281 caactgattt cttcaataag gcatcctcgg ccatcttcaa ttccgtctga atttcgttaa
    11341 gctcctgaca aagtgtggcg ttggcaaagt cttgctcagg cagcttgcta aggtctgtat
    11401 gggcctcttt catgaagcgg gaccaatcat cctgcgtctc ggccaactcc ttaaggatct
    11461 gttcctcctt ttcggtaaag tcttcgacag gtttcttaga caattcttcc gaggcctcga
    11521 tgaccttctt ctgttgttct aagaactcct ggagcttgct atgaatctcc tccagtttct
    11581 tcttgacgtc atcaggcaga tcaccacccg ctcgtttctc ggcctctttg aggagctctg
    11641 tctgggctat acgcgcggca tcaagaatct ttcgcaatgt ctcgatgatc tgatcttgat
    11701 gctgccctaa aggcaccatt tcgtccttag cctttgagag gtcagagagt ttgaccagcc
    11761 gatcgcattc ctgtaccgcc tggagcattt cattggcaac gagattactt agcacccgcc
    11821 taatgatttt tcgctcctct gaatccgtgt ccagaatcga cttgaccacg gcctccgtgc
    11881 tgctgcgaat ctcaacttgt cgcgcccgga tcgcgtgaat ccctccggaa aactgtccgg
    11941 gattatcctc aatcgtcctt gtcagagtca ccaatgctcc cttagctcga atctgttctt
    12001 ctaaaatgcg gaagatggca gccctcaagt tttcgagtgt cttaagttcc tctgtcgttt
    12061 tggtttccgt cgaaatcagc cggatcatga cccaagggga ttccgccttt tgcggtcgca
    12121 aatccaaccc aaacttgccc agattgcgct tgtcccaagc caccgcacgg aggaggacaa
    12181 cttgacccgg ttggaaccgc tccggcgaaa ggaccaattc gtaatcgacg aaaggcccgg
    12241 gcgggttatc ccagtcttga agtttgagtg gctcggtttc gtcagaactc cctttcgctc
    12301 cgtcggcatc cgcttgttgg atcttcattt ccaacctgat tcgccccaga ccatagtcat
    12361 cggatgcgcg gaccctgata ggtaccttgt cgcctaccgc tgccgaagac tggctggttg
    12421 gtttaacaat ctccacagtc ggcggctggt ccggaatcac cgtaatgcga ttgatgcgcg
    12481 gcgcacgctc cgttaaaccg gccatgtttc gtaactttac ggcgaactcg ccgtcgcgaa
    12541 gtagcggaaa cttgacaaga aaggcacgac cgccgtcttc ggtggtccca atcaacggct
    12601 gtccgtcaag cgtgatttct cctcctgcga tcgggaccga gggaatgatt ttcagcgtcg
    12661 cctcggaaaa ctgcggcgcg cgcaaatcgg ccactcgctg ccgaaacgta tcgcggggtt
    12721 tttccatgta gggcggataa tccagagtga cttcgacttc tgcgatcgac ggtatctcgg
    12781 agacgcccaa ccggtagagc ggggactgac tgtcacccac ttccacgcgg tacttcactg
    12841 gcttcaaaac cgagggaagc gagaagctat agttttctaa atccttatcg acagccatcg
    12901 gaaagcgctc ttccttcttc ccatcttcga tcacatagag aaaggcatcg taaggctttt
    12961 tctccggcac acgaaccttg gccgaaatcg tcacattact tccgacaagg acttcagtat
    13021 ttccggggga gacttccaga atctggaccc tccccacaac cggcacaaaa tgccatggtt
    13081 gcattaatcg ggtggctgcg gacccccagt tcgggagcat caagctacat agcacgctga
    13141 ggaggatcaa actcaccaaa atgcccgtcc actccaatag gtcccgagag gtatgtatcg
    13201 cgtagcaaaa ccgctcccaa cgggtatgcc gcgcagccgc ctcttcaaag cgaatccggc
    13261 tggcctggga agccgcatga ttgaccgctg ccaggcaaaa ggcgggatta cattgtcgtg
    13321 gatcgttgag aagctgcaag acatttatga gggcattttg aagctcagga aattgatctt
    13381 caatccggcg agcggtggcc tcaagactgc ggtgacttct cgccacacgc cgaacgaccc
    13441 agacaagcag cccaagattc accgccatcc aaacgaggaa agcccccaaa agcacaggct
    13501 gcgacaaacg aagcaaagca tccgccaaca ctaagattag cagggtggcc agtgaaaccg
    13561 agaccgccat cacgcagctc gtcaacaacg taaaaaacca ccactgctga cgcgtcgcgt
    13621 ccagccgacg aataactcct tcagccgccg gtgacatcat gatgcgtcct ctccctgttt
    13681 ccgaaagact gagaagttta ggcgaaatca cctctcactt atcattgttc ccaccggtag
    13741 gggggacgtc aacactgttt atcgggattc tgcgtcgcca tttcttttct ttgccgaggt
    13801 ttcaaagtgt tcgatattcc gtttctcggg gttggaagca aagagggatc gcttccaggt
    13861 cccgccgcgg aatttgtcaa aatgatgccc gtggaaaatc gtcctaaatc atgaacaatg
    13921 gataatagaa aagggttcgg ctaattcaaa tggcgctcga tgtcgcaaga ctgattggaa
    13981 ctggtcgtaa gccaacgaaa agttgggaaa ctcggttcga aaaagttcgt gcgagggttt
    14041 ccactggtgc ggaaacgggt aaatgttgaa aaaggagccg agacgatgcg gccgaggtgt
    14101 ttttcatacg ttttgacaga tcgtcacatt gtgcagttac catttataag caagatcgcg
    14161 ggcctcatgg ctgcagtgat cgcagttgga ttcttgtggt ttggggacct agcaaccgca
    14221 tcagagccag ccgctatcaa tccgtttgga ccgccggtca gcgagcggga ggacgccgtg
    14281 ccgggatacg tggagctctc tgatgggaaa atcattgccg gcaaggtcta tatgacacgg
    14341 gacaaacgcg tcaaagtatt tgatagcacg ctccagcggc aaagggaaat ccctctcaac
    14401 agaatcaagc aaatcgagtg catcgtcgta agcgagcgga tggaaaagga atggcggttc
    14461 agggaaacaa cttccaacgt gaaagaatac acgggccgga gttatcccac ccgagagtat
    14521 ctccaccgca ttaccctcga cgacggcagg acgattgaag gtccgctttc cgaggtcatt
    14581 tatgtccagc ctgaacctag cggatcatcc tcacgcggcc agcaagctgc cattgaaccc
    14641 cagcggttca tcctttataa gacgcacaaa ggccctgttg gctcggacct taagtctctg
    14701 gtttatgtga aggtggtaaa gctgggggaa gaagctcttg ccgagggaca gaagaagggc
    14761 agtcatagca catcaagtcg cacgacgtcc caagaagcgt ccgagtccaa gagtcgccca
    14821 taaggttgct ccgttgttcc tagcgggaat tgtcaataag aggtgcctgt ttctctgtgg
    14881 tcgaaatctc cctagctaat ggcgcaagta tcgtcagctt ggtactagtg ctcgcggtag
    14941 cagcgggact agtcaccgtt ttctattacc ggaaatttgg cgaactgaaa cggggtcgct
    15001 ggcagttatt gctccttctg cgactgctgg ccgttgtgat cgtggttttg cttctctttc
    15061 gccctgtcgt tcacttctac cgcgaggagc gacaaaagcc caccgttgtc ttcctcctgg
    15121 acacatcggc ctccatgaag atcgctgacg atgtgtcagg gattcctcga tttacccagg
    15181 cgcgagataa aatcctcctt tgggcagaaa ggttgaaaga gcattttgcg ctgcaggtca
    15241 ttccttttgc ggaacgggcg ggcgaaacca tcgccatcag ccaattgggg acgttgtctc
    15301 cggatggtcc agccacatcg ctttcgcggg cacttgtcgc tgccacaaaa gcagcgcccc
    15361 gatcggaaat ggaggccatc tttcttgttt ctgatggaat ccacaattcc gccagaaaac
    15421 cggaggaagt ttcctcccgg atgcaggtcc ccgtcttcac tatcggcgta ggaaccagtc
    15481 tgaaaacaga cgccagtttc aaagatattc aaatcacagg cctggactgt ccgtcacgca
    15541 tgatgcttaa taacctcacc cgaatcaagg ctttcgtgga agcagttggt ctggccgggc
    15601 gagtggtcaa agttgttttt ttggaagatg gtcaaactat tcaggagcag gagcttgttc
    15661 tagatgatca acccggcccg caagaggtca tgttcgaata tcggcctccg gtgaagggac
    15721 ggcacgttta cgcggtccgc atcgatccgc tgagtgaaga gaaaatcccc gaaaacaatg
    15781 agcgctcggc acccgcgttg gtcgtcgagg cccaaatcaa ggttctctac ttggaaggga
    15841 ccctgcgcgc agaatacggt gccattgtgg atcgctttct ctccaaagac cccaacatcg
    15901 agttctatgc cctggtacaa gtgaagaaaa acacttttgt gaagcggaca aatatggaag
    15961 gccttacact ggagagcatc ccctccgatg aggccacgct aaatcaattc gatgttttta
    16021 ttatcggtga tttggatagt agttttcttt cctccgccca acaggaggcc attgtgaatc
    16081 gcataaagaa tgggggcggg ctcattatgt taggcggtta ccacagtttg gggccgggtg
    16141 gctacgccaa tacgcccatc ggccaaatcc tccccgtgga gttgggcgac cgggacatcg
    16201 gacaactgac cgatcccttc ttgcccgaat tgacacccga tggccgacag caccctattt
    16261 tcgccaatat cgcacgtttc ttccccactg gcggtagttc cgccgagcag cagcagttac
    16321 cgccactcga tggctgcacg cgggtgggac cggcccgggc ggcagccacg gtattggcga
    16381 tctgtcccct gacgggcgaa agaatgcccg tcctggccgt catgccggta gaaaatggga
    16441 gaactgcggt ttttacgggt gataccacgc gaaaatggca gcaggtccct cgggtcctcg
    16501 accaggagtc gccattcctc cagttttggg gccaattcgt gcgatgggcg gcagggcggt
    16561 ccgaacaatt ggaggccaag gcgtccgtca cagcagccac cgacaaggcc gcctatgagc
    16621 cggatgaacc tattcaactc tctgccgtag tcagagacca acggggcgaa ggaaccgaca
    16681 aggccaaggt cacggcgaca atccgtgccc catccggtgc tttcgataag gttgcgctgg
    16741 tcagtacgcc tggaccaggt ggtcactatt cggccagcta cgagccgcga ttccatggga
    16801 cactggaaat cgaggttcaa gcggagctag aggatgagaa gctcaccgct gaaaaattga
    16861 tcgtcgaggt tggccgtccc tatctcgaat ttgaaaagct ggaccttgac gaaaaaacac
    16921 tgacgcaaat tgccgccgat actggtggtc ggtatgccca tatttcggct gccgatcaat
    16981 taattgacca actcaaccag gcagagcgca aaaaacgtat ttacgtcgaa caacccctgt
    17041 tctggccgcc cctattttgg gccatttttg tcgccctggt aaccacagag tgggtcctcc
    17101 gcaggagatt ccagctaaga tgagcccgca aagcgcgtac cgttacgtag aaccatcagc
    17161 cctagcacgg gtcaagaatc tccagcttgt cgcccgaggt gtcgtggaag ggtttatctc
    17221 gggcttgcac gccagcccgt acaaggggtt cagcgtggaa tttgcagagc accgggaata
    17281 tgtgcccggc gatgacccac gtcatctcga ttaccgcatg ttcgctcgat ccgagcggct
    17341 ttatgtcaag caatacgaag aagaaacgaa catgcgtgtt cagatcctgc tcgatgtgag
    17401 tggatcgatg aactatcgtt atgaggccaa aattaccaag ctcgagtacg gatgttattt
    17461 gacggccatc ctggcctaca tgatgacgcg gcaacaggac tcggtagggc tcacggcctt
    17521 cgatgacaca attcgccttg atatgccggc ccgcagctcg cctcggcatt tcaatgagat
    17581 gatgcggcaa ctcgaaaact tgcaaccggg caagacgacc ggcctggccc aggtattaca
    17641 caagcttgcc aatcgtttca agaaacggtg tcttattatc cttatcagcg acctctacga
    17701 cgacgtggac aaggtgatcc gagcgcttca tcactttcgt caccgaaaac atgaggtcat
    17761 tgtgtttcat gtctttgaca aggcggaact tgatttccct ttcgatgacc tggtcgtttt
    17821 ccaggacttg gagacggatg aaaaaatcca agttgatcct ttatacgtcc gcgaagaata
    17881 tcaaaaattg ctcagcgagt tcgtcgcgga gtatcgacgc gcttgcttgg agagttccat
    17941 cgactacgtg atgacagata catcggtccc gtatgatttc atgctctcgc ggtatttgac
    18001 gaaacgtaac caattatgac attcggtgcg gcggcatttc tgttggcagc cttggcggca
    18061 atcattccgc caattctgca catgatcaac cggcaacggg ccaagcagtt gccattcagt
    18121 acacttcgtt tcctgaagat cagtgttcaa aagacaaggc ggcgccgaca aatccgcgat
    18181 ctgtttctta tgctcgtgcg aatggcggtc ctcatcttca ttgcgattgg acttgcccgc
    18241 cccaccttaa gtaaacttcg gaattttatg gggggtgggc aatctgcgat tgcgattgtc
    18301 ctcgacaact cggcaagcat ggggtggatc gacgagggac gcccccgatt cgagtgggcc
    18361 atcaacgccg ccaatcagat tttgaatgaa ctccaaggcg gcgacgaagt caccctctgg
    18421 gttacaagtg gccctcgtta ccccgaattg ggccaagtgg atcgcactca cgagaaagtt
    18481 cgtcaattac tggggcagat cgcgcagcgc ggccccacct tcgaacgggg tgaactggga
    18541 cacctcgtgc gcgaggcacg ttatcagctt ctccagtcga aggccgccaa ccgttacgtc
    18601 tttgtcatca ccgacaacca agcggtggcc tgggaagatt tgtcaatgga cgagggcacc
    18661 gcagagaaga ccgcggatca agaagtggag aaatccaaag acccgcattt gcgacagatt
    18721 cccatcatca ttgtcgattg ccagcgaaat ccgaaaccca atgtggccat ccgtaacgtc
    18781 gagctgtcca cgatcctgcc cgtggctggt attcccatga aggtcatcat cgagctattt
    18841 aacagctcgt cgattccgca acaacgggtg gcagaacttt atatgaatgg cacgaaagtc
    18901 ggaagtagtc ccgaagtatc gctggccccg cagagcacgg ctgtcacaga gatcgtgttt
    18961 accctagacc gcgggggaac acaccgagca gaggttcggc tcagcggtga ggatggagcc
    19021 cgttatgacg atcgccgctt tctttccctg gaggtggatc ctgccatccc ggttgccatt
    19081 gtcacaagcg aaaggcatga aatcccctat ctcaatgact ccttttatct cgaaaaggcg
    19141 cttcagcccg tcacggatga atcgtgggca ttaaagccat ccatcttgac agccgcagca
    19201 ctgacaagtg atcccctgtc aaattatcgc atcatctacc tggtggattt gccggaaatc
    19261 gacaaaccga tggccgcgcg attggtagag ttcctccaaa gcggtggcaa gctcgtttgg
    19321 atctgcggtc aggacacaca agtggcctcc tacaatgccg ccaacgaggc cgtcaatggc
    19381 cagctcttac ccgcagcgct cgatagcgtt atggcggcaa gcacggaaga gaatcgagat
    19441 agttggcaga tcgcttggat cgatgttcag catcctgctc tggtcggttt gaacgacccg
    19501 ccatccctcc atcaatcggt tctcgtttac aaattcatta agactcatag ccccgtggga
    19561 agtggagccc gggttctggc acgcctggac ggcgctggag atccccttat tctcgaaagg
    19621 gcggtagggc aggggactgt tgtttgggtg gggacgagcg tccatgtcga ttggacgaac
    19681 cttcccttga gacccttttt cgtccccttt atgacccgcc tggccttcta cctggcggga
    19741 acggaaccgg aacgattgaa ggtcctggca ggacaaccga tcgtgatccc gtaccagggt
    19801 cagattcccc catctgttgt tgaggtcgtt ccacccgacg gcgccctgtt acgactgaat
    19861 ttggaacgcg ggcagacgcc tgagtttcgc tacgtggaca cgcacgcggt cgggttttac
    19921 caatttcgac tgctggcctc ggtaagacct atccagaagc tctatgcggt caacccagac
    19981 ccggacgaaa gtgatccaga aaagatttct cgggaacaac tagaagaggc tttctccgag
    20041 acgacggtcc ttttcgcaga taatcccgaa gacctggccc cgacttttgc cctcctccgg
    20101 cacgggcgaa gcctgtccga attgtttttg acgatggtac ttatcggcct cgttttcgag
    20161 acgtttatct ccaactggtt cagccccaag gagcaggacc agcaacccaa acttctctcg
    20221 caataccggc cccgccagcg actcagccga accgtgggct aaacggctac gacatcagca
    20281 ggagatatcc tgccgaaaaa ggtactggcg ttttactgaa gaacgtctaa gttattacca
    20341 agtcggattt cgaacctcgt tcggcaacga gagaatcgct ttcgccaatt cccgccgggc
    20401 cgcttcgtag gcttgtgggt cgggattcca ttcaaaccat gacggggcaa gctgctggac
    20461 aatcttcagg gccttttcgc cttggccagc acgctgcagg atcatgaaat agtcatagtc
    20521 ttcaatccca tcgcgcaagg cctttaaacg caggctgggg acaatcccgt catagccaca
    20581 cggcttcgcg ggataaacca acgtcccctc gccattgtag ataagtggcc gattcgaacg
    20641 tcccgggcga taggtccggg ggtcagtcca cggatcttcg acctgcgacc agtgactcat
    20701 gccaccccaa taaaggagcc cacgaatatc gtagcgataa gacatccagc acggcacccg
    20761 gtaattgagg agcggataat caatgtgcca ccaaggcgtt ttggcgagct ggcacagagc
    20821 cgtataagcc cacatgattt cgcccagtgc ctgtcgttct cgggccgtgg gctcgtcata
    20881 cagtggaaag agcgagcacc aaatgtccac ggctccataa aggtctcccc aagccgggtt
    20941 ttgagtccgc gtttgctcaa caaccattac tttgactttg gttttcgtag cccggatggc
    21001 ctttccccac acacgtacaa actcgtacgc ttccgggtcg ttcggctcat ctttcaggta
    21061 gatatagtac accacatgcg gtcgatcgat ggactcgatg gcacgatccc atgccctcgc
    21121 ccacgcttgc aacgcttgat tgtttgcctg tgggtctttg acgattccga ccgggctcgg
    21181 gacctgaatg gcattgacgc aatacgtgtc aatccattct cggagccgtg cgacattctc
    21241 gtccgacaag cgaaattctt ctccctcacg ttcaagcctc aaccaatcac taggtgggta
    21301 agcattgatc cgatgctctt gggccaagag ggcacactgt ttctctacgg tggcccaatc
    21361 cacctgttcc gattcttttc cctctttgga ttgtcttgcg tagtacgctc tcatccgttc
    21421 tgctggagaa cccaatgctg tcaccaaact aggcaccctc ggcaacgtga aatcccaaac
    21481 ctccagattg accggcatcg ctacgacctg cccttcttgg gacattactt tcaatgtgcc
    21541 gcgataggtt cccggtggta cgtccttaga aacaaacaca tcaacccaaa atccgtgtgt
    21601 ctcattggca ggcaaatcaa agggctgagc cgtgaaacgc ggcccggaaa ggggttcacc
    21661 tgtgagagga tgccgtgccg gtatcaaggg atcgggatac cagtcggccc gaaaggcatc
    21721 gtttcggtat gttggttcct ttaactccaa ttgatgctct cgatagatac gaaaacaggc
    21781 cgagtcgatg ttgcctcccg attggccttc caacggcgac aattccacgc cgacagcggg
    21841 aagcggatgt gaactccgca gaaacacctg gaaactcacc cattcattgc gcgctgccgt
    21901 cagattaact tcccgagaag gggagggcgg gggtgtttcg ctgcggcaaa ttcggagggt
    21961 tgacgtctca atccaaacaa cgggctgggc ctgtgccgca ttagccgaga gccctacgaa
    22021 ggataacaac atcgccaagc taaggtaaga aagcttcatg gttattccct ccaaaagcca
    22081 aaggattaca aggtgggaca cactgccaga ttcacgccat caaggtttgg aaactgctcg
    22141 ggaaacacgc ggcagacgta tcttccggct tttgcaaacc ccaggatacc cattcgccgt
    22201 tggtccctgc aatatggagg tggaagcgtg ccccgttcgt cggtgcatcc gtgaactagc
    22261 cacatgccta gctgtttgtt gcaactcgtt gtctgatagt cgcttctgaa tctttcaata
    22321 gttcaagcca agacgttggc ttcgagagat accgcgtcgc ctgcaggaag aatccgtaac
    22381 aaggaagcag gttcgggcta gtccagaaac ggactcgcga atcaaacgct gacggcaatc
    22441 ttcgagggtt attaccccgt cgaatcgttc ttgccgggat gatcagtcat cattccaccg
    22501 cgggcatttt ctggcacagc agcgagaaag accgaaaaaa tagccgaggc gaaatgccca
    22561 aggacataga taaggtacat tgccccaggg agtacacccg ccatcaccag cgtttctcgt
    22621 ccccgtaata cgcgaaacgg tggttcccta acgtcgagcg aagcttgaac cagctttcca
    22681 tccgaagctt gctggccaac tgcggcgtag gcctccgggc ggttcaactc cggaccgtca
    22741 ataatgatct ccatcaccag ttgcaggtta cccactagaa ccatgacgcc gaacacgacc
    22801 ggggttaacc gcacaaggat ttttagtcca aactggatca aaccaaggat ctcgtccgcg
    22861 ggcgatagct ccgggctaac ctgcaagttc ataccctcca ggttgagaga ttgtaacgcg
    22921 acaaatagtc cccaaatgaa caccgctacg ccccaaaaca ccagctcaaa tgacccagtg
    22981 atcaaggcat cgatagcccc caccacgagc atagctgatg aaccgaccaa cgacaaaagg
    23041 gacagggcat cgggaacaac ggaggaactg agatacccat tgatttgccg atcccatcgt
    23101 tctaagacaa acaggaaccg cccgccgata tagtgaaaca caagaatcaa caaggcaagg
    23161 ctaatcccaa ttaagattgc ccacgcttgt ccaccggctt tctgaaggca ttggaaaacg
    23221 accaaaacgg cgcccaccag taagccatat ttcccacaga taagaaacaa gtgcgcactt
    23281 gaacgaagaa attcacttcc gaaccgccag cggagaaacg ccaaagctgc gtcaagcgga
    23341 tgacgactca ggctctctcg tgatcgcgca aacgtcgtcg gcgagcgttc aaatgttcgc
    23401 ggcgaccgca cagcacgacg accaccgtcc ggtagtggca ccgacgaatc gtgagcgtcc
    23461 cctggttcga gcatcattcc cggccgacct cgttgcgctc cgggcattcc ggatgacagt
    23521 gacaaaaacc cgagcgttta tgatcacgct ttccccgctt ccccaccttt ccataacgtc
    23581 cattgtgatt atcatttgtc agaataacaa acggtagatc tcgagattct ttgcatgtac
    23641 tttcggagtg ccggactggt ccataaaacc ctgacatcgg actaaaatca cctagggttc
    23701 catcctgtgc aagatttgat cctggaattt ttacacaaat tcatgctctg ttttgttccc
    23761 ctttttgcgg ctgtcgatgc agcagggc
//
LOCUS       DAOSUT010000059        23717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1397_length_23717_cov_9.883949, whole genome shotgun sequence.
ACCESSION   DAOSUT010000059 DAOSUT010000000
VERSION     DAOSUT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_23717_cov_9.883949"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="hemC"
                     /locus_tag="PLD05_07180"
     CDS             <1..796
                     /gene="hemC"
                     /locus_tag="PLD05_07180"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOP77256.1"
                     /translation="GVFTKEIQLALLANEVDLAVHSMKDLPTQPVPGLAIAAIPKRGP
                     VGDVLISRTAQTFEQLPSEAIIGTGSRRRAAQILHYRKDLTISPLRGNLDTRLRKLDS
                     GLYDAIVLAQAGLERLNITGRMCEPLTPRFLLPAVGQGALAVECRLDDEETRQCLTVL
                     NDPISAAEVTAERSFLRHLEAGCLAPVAAWCRAVGSSLLLTGRVLSVDGTTKLEVSCL
                     GDIENPEELGRRVAQLLIAQGAKDLIRQAKTIPMAPPPDAKPDIDD"
     gene            1045..1806
                     /locus_tag="PLD05_07185"
     CDS             1045..1806
                     /locus_tag="PLD05_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOP77257.1"
                     /translation="MPYGIYLSAEGAHAQDRRLTILANNLANVETVGFKRQLAICQAR
                     YTEPVNRGLESPGTGRWSDLSGGVVVSMTKTDFSPGPLKETRSPTDFAIRGEGFFTVQ
                     KGDEVFLTRAGNFQLNGKGQLVTQYGGEQYLVLNDAGQPIVIDGSRPWRVNPQGVVEQ
                     EGLLQNIAVVRPASKDSLVPVGENLFRPTAQPSPVPLAQRQVAQGYLEMSSVQPTLAM
                     VELLEASRLLEANLAMVQTQDQMLSNLFNRVLRAS"
     gene            1873..2676
                     /gene="flgG"
                     /locus_tag="PLD05_07190"
     CDS             1873..2676
                     /gene="flgG"
                     /locus_tag="PLD05_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOP77258.1"
                     /translation="MSVQTLYSAATGMLAMEKKLDVIANNLANIETTAFKKGRANFED
                     LFYSHEVFPGAQDTSGEMTPVGISIGLGSRLSGIQTDFRQGAFQQTSNQLDVAIEGAG
                     FFQVMDPSGQIYYTRAGNFSINANGNLVLGSAHTGRLLEPPIVIPNDATAITITPEGQ
                     VYVQQPNNPQLTLVGDIQLVNFINPEGLLKLGENLYAETDASGPPQAGTPGRDGLGLL
                     RQNALEASNVEPVRELIDLITTQRAFEMNSQAVQVGDQMMQLVSNLRRY"
     gene            2696..3751
                     /gene="flgA"
                     /locus_tag="PLD05_07195"
     CDS             2696..3751
                     /gene="flgA"
                     /locus_tag="PLD05_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOP77259.1"
                     /translation="MKRWCIPAVILGAFLLWAGASASAATFRLRSDFTATRTPITLRD
                     LVAIEDCDPETRDALGSLPLFPAPPIGEARFVNRDEIRDLLILRGIDMRMHTWTGAFQ
                     IRIVTSPTAPTPPEREKNSSQLQTRAQERLTAALEDYWRRTFPESPKCRFEFVLSPED
                     VKWFSAADASLVVNRVQALSADTWLVDVLTQVGQEKNVTQIKVKCGPPSQVVVAARPL
                     SRDVIIGRSDVALSERAGPVDSEIATQIEEVVGKQLTLAISAGKPIPRSALRDPVVIR
                     RGDLIQVTVRASGVTVRTAARAKDDGVVGHLIPIETLDGKGKTIMARVVGPKQVEIYA
                     MSPEAEASKNLLSRGGL"
     gene            3790..4458
                     /locus_tag="PLD05_07200"
     CDS             3790..4458
                     /locus_tag="PLD05_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOP77260.1"
                     /translation="MSVAQHGLLAITVLLCALFHQVALGQTSSLLGGSQGTPPTLDRL
                     SWTYQPPPQQKVLRLHDILTVLVDEKTQVIAEGQMDRRKKADGKFVLSDWVLLKKWSL
                     IPDPQSLGDPTIGGQMENKYRAESELESREAMKLRVACEIVDIRPNGNLVIEGRRTIQ
                     VNNDIWEIYLTGTVWPQSILPDNTVQSENVADLRVIKREVGHVRDGYRRGWLLKWLDE
                     HQPF"
     gene            4504..5553
                     /locus_tag="PLD05_07205"
     CDS             4504..5553
                     /locus_tag="PLD05_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOP77261.1"
                     /translation="MTMRHRFIGAIVCLCVFMTGGALWAAVPLKTICRVKGQEDNNLH
                     GLGLVVGLKGTGDGSSFLPTIRSMAVALEMLGNPLARDAQTELKDAKNVALVLVTATV
                     PPAGARQGDRIDCQVHSVGSCKSLAGGRLFLTPLVGPDPRDRRVYALAEGSITIESTD
                     TPTVGRITGGCRLEADFFNPFVKDGRVTLVIDPPYADFQVAQDIVELINSQMTVQSGG
                     MMLARAINQVNVEVLIPRQYADDPVLFVSQLMELPLMDVAVGPRVVVNERTGSIIISG
                     DVEIGPVVVTHGNMVIQAGGAQFVSVDSAQTQTTKLKALVDALNALNTPPQDIIQIIK
                     GLEKNGKLHGRLIIE"
     gene            5587..6084
                     /locus_tag="PLD05_07210"
     CDS             5587..6084
                     /locus_tag="PLD05_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOP77262.1"
                     /translation="MTMLRSEAVSQTHPLAVIRSPSNGRSRENTSQPFVAYLDAVANR
                     TAAPSPFWTGNAASAQFESRDVTTPPQDTQQDKQGHGISPTNSEDEELEKVFRRFVGQ
                     TFFGLMLKEMRKSIHKTPYFHGGLAEQIFEQHLDMTLAEKLADASGDKFSKPMYELFQ
                     LQRRG"
     gene            6170..6658
                     /gene="flgN"
                     /locus_tag="PLD05_07215"
     CDS             6170..6658
                     /gene="flgN"
                     /locus_tag="PLD05_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOP77263.1"
                     /translation="MHEEWERELAKFLQEVTTIQGRLIDLLQRKQRLLAGADVEGLAA
                     LAPEEGTIEEQLKQCLARRAELLEKAAGQGLPADRITNLAGAVGASRQLKRQLAEAAH
                     QARLVQIHNLTNWMLNQRALIHLSQLIEIIATGGRRHPTYHYDSNQSLPETQGVLVDH
                     NA"
     gene            6703..8400
                     /gene="flgK"
                     /locus_tag="PLD05_07220"
     CDS             6703..8400
                     /gene="flgK"
                     /locus_tag="PLD05_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOP77264.1"
                     /translation="MSLFSSIRMASNTMRADQIALQVIGQNIANAETPGYIREEIVLK
                     PAPIQKLGGLPLGLGVQVAAVIQKLDQFLEERLRASVSDRAGAEVTEETYHQLEGLIG
                     ELSDTDLSSDLTRFFSSISEVLNQPESVSARNLVVLQAQTLTQNIRRLHDRVNAIRSD
                     LNKRIDDVADRINRLIEDIRRLNIRIAETEGGDVSQSDAVGLRDQRLQALEELASLID
                     IRVEEQPSGGVAVYSSGFFLVYEGTSREVTVVRSTDRGLGVSEVRLAETDSPLEVHAG
                     ELYGLITARDSVLGSFLDRLNEFTAALINGFNRIHASGQGLSGFTQLTSVEGIADPTV
                     PLNQAGLRFTPQNGSFLIQLLNTRTGAIQTTRVRVNLAGLDNGTTLLDLADQINAIDG
                     LQASIDSLGRLNIQCTGPDQVFSFAEDTSGVLASLGLNTFFTGTGAGDIGINRVLTQD
                     PSKFAASKNGIGIDTETAVALAGFADSPLTEYGGRSINALYDQMISEVTQGAAISRAG
                     AESARTFETTLRGQKLAVSGVNLDEEVVKLIAFQQQFQVSARFIKTLNELIQILVNI"
     gene            8481..11492
                     /locus_tag="PLD05_07225"
     CDS             8481..11492
                     /locus_tag="PLD05_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOP77265.1"
                     /translation="MPSVTGVPTTRITNLFVRQRLLQQIQADQRDIVNLQLQLSTGQR
                     FSVPSEQPIVSLRVIGLQRLLEQKEQVKANLATTQSYLTAADTALSSISSMVAEARAT
                     ALGVLGTTATDTQRAAAALQIQQTIQQLLDAGNQKFRGRYLFSGSLTDLRPFGVTGNN
                     LVAFYGNERLLPSYVDTDLLSENNVLGSQIFGAISSAIEGTADLQPRLRFDTPLAHLN
                     NGAGVDLGSIVISDGTRSAVVDLSSAHTIGDVAMLIRRNSARIPLNVEVTSQGLKIQL
                     NAASGDLSIRDVGNGTTARQLGIYREIGVGTSPIIGSPLNPCLKPTTQLKDILGSPAR
                     AALRFPGSDNDFYLEADSNGDALNGVKIRFIDDPSVTVGSEIVEYDAAAKELLIRIDE
                     THSQARDVISAINNAYDAGQLPFFAYLDPTDQELYPGQGLVFPTPAGEWAAVTEGGSG
                     EDFDKTSGLLITNGGQTFAVDFSKAATIEDVLNTLNASSYGLLAEIDESARRINVRTR
                     ISGADFAIGENGGKTASQLGLRTLTADTRLDQLNFGRGVSDYRDQGTTARAIFDPVGT
                     HNRLILQARTAGPEFNGFRLSFYDTGGPPGSEIITYDSVNKQIAIGIVPGSTTAKQIV
                     ELFAATPGARDNFQLRLDDESLDNDGSGLLQVGEVLTSGGSSGGIDFTITRADGVVME
                     IDLSGAVTVQDVIDRINNHPNNPPRVPGGTPWLIARLAQYGNGIELIDESVGTGVLTV
                     SRTPSSTAAIELGLVPVGAEYAKATSPGTIGQANVRSSGANNDLIFRTKSPTASGNGY
                     RIIFEDSGGGPESFTFDPANKIMRFAIQPGVTTANRIIELFSSDPVAPLVFDALLDPT
                     DGNDGTGFVEVTSPSSPPTIAGGQSSQLTGRDVNPLETEGIFTALIRLKDALIRNDVW
                     GAQRAIDLLDEAVLNLNFRRAELGAKQQSLDILNNRLEDEDTQLQSALAADFDADLAE
                     VISSLVAKQSAYQAALQATAKIFRMSLLDYL"
     gene            11733..12344
                     /locus_tag="PLD05_07230"
     CDS             11733..12344
                     /locus_tag="PLD05_07230"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOP77266.1"
                     /translation="MSNDQENLARAKQELRQRLKAVRQSLDPEWVLQASQAIQETLVQ
                     LPEYQSSRSIMSYVSTGKEVETHRLLLRMKRESKVVVIPWCEGDELRLFRWESFDELD
                     RGTLNILEPRPDLRSKETKIFSPHELDLVLVPGLAFDRNGNRLGRGRGYYDRFLKTLP
                     RRVVRIGLAFECQLVERVPVSPNDEKVDLILTEFRTYRVPRID"
     gene            complement(12428..15295)
                     /locus_tag="PLD05_07235"
     CDS             complement(12428..15295)
                     /locus_tag="PLD05_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77267.1"
                     /translation="MSKQRCKNTPEHRDLASGPSLVSSGALRVFQVTRHLIANVTGFC
                     LRERSPRRWFLFVAIVLVGVALVVFLDWWITVPDSVSVTYVGRESCYRCHEQECRLWE
                     GSDHDLAMDVATSETVLGNFDDCTFTHIAFSDVWRFSDEELRKILSRLDDETLAVALQ
                     PYEVAHTPETFYPYTPPSGDRSRQRILEVLPEERRSAVERLVTWKKTFARPCDITDAH
                     RKLGDAARDLIQSGQIENPDWAVTSRFFRRNGKFYVLTDDRDGTPREFQVDYVFGVYP
                     LQQYLVTFPDGRVQCLPLAWDVKSRRWFHLYPSDPIPAQDPLHWTRALQNWNYMCADC
                     HTTNLQKNYDVTTNTYRTEWSEIDVSCEACHGPASLHVQLADSWSLFWDRKRGNGLVS
                     FSPQKCDNKTVVDSCAPCHARRRPIASPFPPGEAFLNYYVPELLDGNLYYPDGQILDE
                     DYEYASFLQSLMYRKGVRCADCHDPHTARVKFAEKAKVGEVRQPYADNKLCGQCHLPS
                     KYDTVQHHHHPDSTKPGTHCVECHMPETTYMVVDARRDHSLRIPRPDLTVSLGIPNAC
                     NLCHQDPEKGETPDWAVEWVNKWYGPRKEPSHFAYAFEKGRRLDSSGVIELLAVARRQ
                     DLSAIVRASAVLLLANYGSEAARGAVFAAARDPEPLVRLAAARALQNVVIREDDVPRV
                     QHLLSDPIRAVRVESVPWALNLPPQALSGSAMKALQSAIEEYRTSQLLVSDQPGAHMN
                     LAILAEFLGDFQEQEQKYLDALRIDPLFLPARNNLGMIYARTQRFAEAEEQYRRALKI
                     NPDFVPVRDNLARLYYQMGRFDEAEREFRTILSKYPNRGDIHFALGLLLAELPTRLAD
                     AIEELREAARLLPNESRVLYNTAVALQKAGRASESAEYYEKAYRLGNPSADLLQGYAL
                     CLVELKRCAAALAIADELCQRFPNPESAHFRNAIAERCRSN"
     gene            complement(15322..15975)
                     /locus_tag="PLD05_07240"
     CDS             complement(15322..15975)
                     /locus_tag="PLD05_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOP77268.1"
                     /translation="MVNLNLSPASMRVVKLLVGNTPKSVADLIREMKVTRTAVTEQLN
                     ELVAAGLVERQMQRLPGRGRPRHVYSATPGALLLLLSKNQEMVLPNIWRAIREVGGPK
                     LLGEVIKQVSRWLAEHYRKQITATTPAERVLQLVEILREEGVLVDIQEENGRYTIRQR
                     SCPYIMMYEENRAACLLDQETFALVLGGPVRQVACRHDGAPCCVFEVTKDNPATDAA"
     gene            16608..17261
                     /gene="trxA"
                     /locus_tag="PLD05_07245"
     CDS             16608..17261
                     /gene="trxA"
                     /locus_tag="PLD05_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOP77269.1"
                     /translation="MKRNQIWQKSGYLTRYEVLSVKLRTVGAFVLAGGLTILGGCTSE
                     ALSGSHLRPESISETLAVTPADQNSVRRSGEGDAISRETTKTDQPFRDTSTNPSQEKK
                     ETRTMWFTGGKNTIEHANAANFNTLVAQSSVPVLVDFYADWCGPCRALAPILEQLAQE
                     VNGVRIVKVNVDQAPTLAAQYRVSAIPTLILFDQGRPVQRITGLASKEDLKRLLALD"
     gene            17611..19311
                     /locus_tag="PLD05_07250"
     CDS             17611..19311
                     /locus_tag="PLD05_07250"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOP77270.1"
                     /translation="MAKSAARQFAIGLDYGTNSVRALIVDVRTGEEVGTAVYQYPSGD
                     KGVLLDPKNPNLARQNPADYIEGFYRCVRAAIRSVPKSVGFAPEHVVGIGVDTTGSTP
                     IPVDENGWPLAFQKRFANNFAAQAWLWKDHTSYAEAAEITEKARAMGLPYLAKCGGTY
                     SSEWFWSKILHCLRTNPNVFDAAYSWVELADFIPGFLTGNLKPETIPRSICAAGHKAM
                     YNPEWGGLPSEEFLRALDPKLVKIRQRYAPEALPADRKAGELTREVARKVGLRPGTPV
                     AVGAFDAHMGAVGAGIKPGTLVKIIGTSTCDMMVVPMNQPLPDIPGLCGIVPHSIIPG
                     MYGLEAGQSAVGDIFNWFVSFLAPAKYTAAGDPHAALTRDAEKLKPGESGLLALDWNN
                     GNRTILVDPLLTGLLVGQTLLTTAPEVYRALIEATAFGALTIIKRFEEYGVTVKEVVN
                     CGGIAEKNPFVMQIYADVCNRPMKISRSAQTCALGAAIFGAVAGGAYKDVEQAQRRMT
                     GTKPTVFRPRKQAVAVYERLYQLYRRLHDAFGLPDYRDSLYDVMKQLIALRKEVRGES
                     "
     gene            19424..20125
                     /locus_tag="PLD05_07255"
     CDS             19424..20125
                     /locus_tag="PLD05_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOP77271.1"
                     /translation="MSLEQLREEVCRANKLLMEHGLVCLHSGNVSGLDRSTGRLVIKP
                     SGVDYETLSPEDLVEVDLITGKVISTHLRPSVDLPHHLYIYRNMPGVGGIAHAHSDYA
                     TAWAAVGRPVPLCLTSMADYFGGEIPCAPYVDNEGDNIGQAIVRYRGRAPAILLANHG
                     AFTWGPTALDAVKLAVALEDAAKKAFLASQLGQPLEIPLEEARKWYDKYHHGYGQIVP
                     PRAPQTVTRQPQSPG"
     gene            complement(20191..21180)
                     /locus_tag="PLD05_07260"
     CDS             complement(20191..21180)
                     /locus_tag="PLD05_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77272.1"
                     /translation="MEKHVSRRQFVGTTLFGSTVGALSCQALTRMTVAEEPASAPVTS
                     LPKGKIKDLEISRLILGGNLLTHFTHSRDLRYVYTLARHYNTDEKILETLAVAEAHGI
                     NTLFIHYVPHALSILQEHRKRGGKMQWITCTAHALVSGGLDEFKRQIDALVAAGTNAL
                     YISGLEGDFSCGFLNDVCGPEADERVGAPRMELLAQALDYAKSHKLPVGIGAHRMGVV
                     EDCEKAKLDCDFYVVTFHRQNYPSAKLYYDSRWCSKPEELAALMQSVEKPWFAFKVMA
                     AGAIPPEVAFRYAFENGADFVLAGMFDFEIATDVALVNKILAELKNRQRPWRA"
     gene            complement(21313..22155)
                     /locus_tag="PLD05_07265"
     CDS             complement(21313..22155)
                     /locus_tag="PLD05_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77273.1"
                     /translation="MIELGFHTDNWRPLSKSFEYAVEMGAALGLKHVEFAVINGQDFI
                     QEMGYDPAVSLQSNPRALRRYLESKGLSVSQIDGAYPLMGPKGSAFGVHYVQQTIRFA
                     AELNCPIVDTTDGAVEVPGLSRDEVFRITCDNYRQCLSWAEDYGIILAVEPHGPYTND
                     PEFLHRLFDYFQSEYLRFNLDTGNTFIAGHDPLEFCKEFRPYLVHCHIKDVSESLAAA
                     ARGQETGIGTSFVPVGGGVNAENIRRVIQYLKETNWSGAVSIECYGTQEFMEASVKFL
                     RSLL"
     gene            complement(22576..>23717)
                     /locus_tag="PLD05_07270"
     CDS             complement(22576..>23717)
                     /locus_tag="PLD05_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77274.1"
                     /translation="KDDPRIDPDPRRTIFDRNIYQGGGAQMGHTYRNIISADGGSGGP
                     QMRLGGLCENSLIIEGYWFSSTASNRLFNPWLTAGKQTGCSAIVRNNVQFVYVYPSPA
                     DPDTDGKSDRRAQPGHGYTMQGASFGGVVENNIVSGAMLTDDPGFSRPVGQAAFSLSP
                     GRGQYEDGNYYTMQRCTIRGNIGYRTKFGLRLTGDWTDTMNLVVENNVFVAEGIVSSD
                     AEHLTSANQLVVRNNRFYVANDDLPQAVWMGTDNTVAPRDEAAAREKWPDPDRTLKRY
                     VQEVLKLTLLDWSDDPWLDRDEVARRVKAGEPYDPTGLKTFMAVATNMRRGGTDPIPT
                     SGKPSWTGDYPWDARFTGQAVVNWVREGFGMKPVEASGESPEKTR"
BASE COUNT         5376 a         6612 c         6574 g         5155 t
ORIGIN      
        1 cggggttttc accaaggaaa tccagttggc ccttcttgcg aatgaggtag atttggctgt
       61 ccacagcatg aaggacttgc ccacgcagcc cgttcccgga ttggcgattg ccgccattcc
      121 caagcggggc ccagtgggag atgttctcat ctcccgaacg gctcaaacgt tcgaacaact
      181 tccgtcggaa gcgatcatag gaacgggcag tcgccgccgg gcggcccaaa tcttgcacta
      241 tcgaaaagac ctgaccattt cacccctccg cggaaacctc gatactcgct tgcggaaatt
      301 ggattcgggc ttatacgacg ccatcgtttt ggcccaggcc ggtctggagc gactcaacat
      361 taccggccga atgtgtgaac ccctcacgcc acgtttcctt ctccctgccg tgggtcaggg
      421 tgcactggcg gtggagtgtc gtctcgatga cgaggagaca cgccaatgtc tcaccgtcct
      481 gaatgacccc atctccgctg ccgaagtcac cgcagaacgg agcttcttgc ggcatcttga
      541 agcgggctgc ctcgcaccgg tggcggcctg gtgccgtgct gttggttcga gtctgctgct
      601 gacgggaagg gtcttgagcg ttgatgggac aacgaaactg gaggtttctt gccttggaga
      661 catagagaat ccagaggaat taggaagacg tgtggcgcaa ctgctgattg cgcaaggggc
      721 aaaagacctg atccgtcaag ccaaaacaat cccgatggcc ccaccgccag acgccaagcc
      781 ggatattgat gactgaatca gtcccagggg ctttcggcag tttctgcaga ttccccggct
      841 gattctgcgt ctcgacgaat gatctctaga tcgccagctt cttgacgaga tcgccgggtt
      901 cttcgtaagt tcttttctga aaaaggttta tgtcaccata ttcatcgtgc atcgtgcctg
      961 gcacggtgtt tgcaatgatg aggcggccgc ggaaaaagga ttttctcgaa acgtcacgcg
     1021 actgaacgga ttttgacgag taccatgcca tacgggatct atctttcggc agaaggtgcc
     1081 cacgctcagg accgtcgctt gacaatcttg gccaacaact tggccaatgt cgagaccgtt
     1141 ggatttaagc gtcaattggc catttgtcag gcccgttaca cagaacccgt caatcgcggg
     1201 cttgagtcac cggggaccgg tcgatggtcg gacctcagcg ggggcgtggt cgtttccatg
     1261 accaaaaccg atttttcccc aggacctttg aaagagaccc gcagccccac cgactttgcc
     1321 attcgcggcg agggtttttt cacggtgcag aaaggagacg aggtctttct cactcgggcg
     1381 ggcaattttc aactcaacgg caaaggacaa cttgtcaccc agtacggtgg cgaacagtac
     1441 ttggtcctca acgacgccgg acaaccgatt gtgattgatg gatcacggcc ttggcgggtc
     1501 aaccctcaag gagtggtgga acaggaggga ctactacaga acattgccgt ggtgcggcca
     1561 gcatcaaagg attcacttgt gccggtgggt gagaatctct ttcggcccac cgcccagccc
     1621 agccccgtcc cgctggccca gcgtcaggta gcacaaggat acctggagat gtcctctgtg
     1681 cagccgacgc tcgccatggt ggaactcttg gaggcctcgc ggctactaga agccaatctg
     1741 gcaatggtcc aaacacaaga tcaaatgctg tccaacttgt tcaatcgggt gcttcgtgcc
     1801 tcctaaagac agcgcaattc aagacgactt gctaacacac agttgacctg agcagtagaa
     1861 acgagggaag aaatgagtgt ccaaacactt tattcggcag caacaggcat gttggcgatg
     1921 gaaaagaaac tggacgtcat cgccaacaac ctggccaaca ttgaaacgac ggcgttcaag
     1981 aaaggccggg ccaattttga agatttgttt tatagccatg aagtcttccc cggtgcccag
     2041 gatacctcgg gcgaaatgac cccagtcggc atttcgattg gcctgggctc tcggttgtcg
     2101 ggcattcaaa cggacttccg ccagggggct ttccagcaaa cctcgaacca gcttgatgtg
     2161 gcgatcgaag gggccggttt cttccaggtt atggatccgt cagggcaaat ctactacacc
     2221 cgggcaggca atttctcgat caacgccaac ggtaacctgg ttttgggatc ggcccacact
     2281 gggcgactcc tggaaccacc tattgtcatc cccaatgatg ccacagcgat taccatcacg
     2341 ccagaaggac aagtttatgt ccagcagccg aacaatccgc aattgacgtt agtgggcgat
     2401 atccaactgg tcaacttcat caacccagaa ggtcttttga aactgggtga aaacctctac
     2461 gcagagacgg acgcctccgg gcccccacag gccggaacac cggggcgcga tgggctgggg
     2521 ctgctccgcc agaatgcctt ggaggcctct aacgtggagc cggtgaggga gttgatcgac
     2581 cttatcacca cgcaacgggc tttcgagatg aactcacaag ccgttcaggt tggcgatcag
     2641 atgatgcaat tggtttccaa cctgcggcgg tactaagtgt tctgaggatc atgccatgaa
     2701 acggtggtgc ataccagcgg tgatccttgg ggcctttttg ctgtgggccg gagcgtctgc
     2761 gtcggcggcc acgtttcgcc tccgctcgga tttcaccgcg acccgaactc ccatcaccct
     2821 gagagacctg gtggcaattg aggattgcga ccctgaaaca cgtgacgctc tgggttcttt
     2881 accgctgttt cctgccccac cgatcggaga agcgcgtttt gttaaccgcg atgaaattcg
     2941 tgatctattg attctgcgcg gtattgacat gcggatgcac acctggacgg gtgccttcca
     3001 aatcaggatt gtcacaagcc ctacagcgcc gacaccgcct gagagggaaa aaaatagctc
     3061 tcaacttcag actcgtgccc aagaacgatt gacggcagcc ctagaagact actggcgccg
     3121 cacgtttccc gagtcgccaa agtgccggtt tgaattcgtg ttgtcgcccg aggacgtaaa
     3181 gtggttttcc gccgccgatg cgtccctcgt ggtcaaccgc gttcaagcct tgtcggccga
     3241 tacgtggctg gtcgatgtgc taacgcaggt tggtcaggaa aagaacgtaa cccaaatcaa
     3301 agtcaagtgc ggtcctccgt cgcaggttgt ggttgccgca aggccacttt cccgcgatgt
     3361 catcattggg cggtccgacg tggccttgag tgagcgagca ggacctgttg actccgaaat
     3421 cgcgacacaa atcgaagagg tcgtgggcaa acaactgacc ttggcaatct cggcgggaaa
     3481 accgattccc cgctcggccc tccgagatcc agtcgttatc cgtcggggtg acctgattca
     3541 ggttactgtg cgggcctccg gcgtgacggt tcgcacggca gcacgggcga aagacgatgg
     3601 ggtggtcggt catctcattc ccatcgaaac gttggatggg aagggtaaga caatcatggc
     3661 ccgggtcgtt ggccccaaac aagtcgaaat ctacgccatg agcccagaag ctgaggcatc
     3721 taaaaaccta ctatcacgtg gtggactatg acccttgatc cgaaatatct aaggcaaaag
     3781 aagacaagcg tgtcggtagc gcagcatggg ctccttgcca taacggtgtt actgtgtgct
     3841 ttattccatc aggttgccct tggccaaaca agtagcctgc tgggcggctc gcagggaaca
     3901 ccgcccacgc tggatcgtct gtcctggaca tatcagcccc cgccacaaca aaaagtcctt
     3961 cgcttgcacg acattttgac cgtcctggtg gatgaaaaga cccaagtcat cgctgagggt
     4021 caaatggacc gacgaaaaaa ggccgacgga aagttcgtgt tgtcagactg ggttctgctc
     4081 aaaaaatggt ccttgatccc cgatccccag agtttgggag atcccactat tggcgggcaa
     4141 atggagaaca agtaccgcgc cgaatctgaa cttgaaagtc gcgaggccat gaagcttcgt
     4201 gtggcttgtg agattgttga tatccgtccg aacggcaacc tcgtgattga gggccgacgg
     4261 acaatccagg ttaataacga catttgggag atttacctaa ccgggacggt ttggccacag
     4321 tccattttgc ccgacaacac tgtgcaaagc gaaaacgttg ctgatcttcg ggttatcaag
     4381 cgagaagttg gccacgtgag ggatggttat cggcgcggct ggctcctcaa atggctggac
     4441 gagcatcagc ccttctagga gaccaccagg ttattttgct aacaaaggga gcatggacgc
     4501 accatgacaa tgcgacatcg ctttatcggc gccatcgttt gcctctgcgt gttcatgacg
     4561 ggcggcgccc tctgggcggc ggtcccgctc aagacaattt gtcgcgtcaa aggccaagag
     4621 gataacaacc ttcacggtct gggacttgtg gtgggcttaa agggtactgg agacggcagt
     4681 agctttctcc ccaccatccg cagcatggcc gtggccttgg aaatgttggg aaatcccctt
     4741 gctcgcgatg cccaaaccga attgaaggat gccaaaaatg tggcccttgt tttggtcacg
     4801 gcaacagtac ccccggcggg cgcgcggcaa ggtgatcgca tcgactgcca ggtccactca
     4861 gtgggatcgt gcaaaagcct ggcaggggga cgactattct tgacaccgct cgtcgggccc
     4921 gaccctcgag accgccgtgt ttatgccctg gctgagggtt ccattaccat tgaaagcact
     4981 gacacaccaa ccgtgggaag aattaccggt ggctgccggt tggaggcaga tttcttcaat
     5041 ccgtttgtca aagatggccg cgtgacactg gtcattgatc ccccctacgc cgactttcag
     5101 gttgcccagg acatcgttga gttgatcaat tcccaaatga cggtccaaag tggtgggatg
     5161 atgctggctc gggcgatcaa tcaagtcaac gtggaggttt tgattccccg gcaatatgcc
     5221 gatgatcctg tcctgtttgt gtcgcaactc atggaattac ccctcatgga tgtggccgtc
     5281 ggtccgaggg tcgtggtcaa tgaacggaca ggcagcatca ttatcagcgg cgatgtggaa
     5341 attggccccg tggttgtgac ccacggaaac atggtgatcc aggccggagg tgcccaattt
     5401 gtttctgtgg actcggccca aacgcagacg accaaactca aggcccttgt cgatgccctt
     5461 aacgccttga acacaccacc ccaggacatc atccagatca tcaagggtct agaaaagaac
     5521 ggcaaactgc acggccgatt gattatcgag tgaatcatgt cttgagcaaa agagtagggt
     5581 taattcatga cgatgttgcg aagcgaggcc gtttcacaaa cccacccttt ggcggtgatt
     5641 cgttcgcctt caaacgggcg gtcaagggag aacacttctc aaccgtttgt ggcctatctc
     5701 gatgcggtcg caaaccgtac ggcagccccg tcaccgtttt ggaccggcaa cgcggcttct
     5761 gcgcaatttg aaagtcgtga cgtaactacg cccccccaag acacgcagca agataagcaa
     5821 ggccatggga tttccccaac aaactcagaa gacgaggaac tggagaaggt cttccgaagg
     5881 tttgtggggc aaacgttctt tggccttatg ctcaaggaga tgcggaagtc gatccacaaa
     5941 acaccctatt tccatggtgg cctggcagag cagatcttcg aacagcactt ggacatgacg
     6001 ttggctgaaa aactggccga cgcgtcgggc gacaaattct cgaaaccgat gtatgagctg
     6061 ttccagctcc aacggcgtgg ctaggacgac cgaccagcgg ggtgacgtgc aactcatgga
     6121 aaacatctga ctcacaatca cgtacgggta gtgtctgagg atgatcgcaa tgcacgaaga
     6181 gtgggaacga gagttggcga aattcttgca ggaggtgacg accatccagg ggcggctcat
     6241 tgacctcttg caacgaaaac agagattgct agccggagcc gatgtcgagg gtctggcggc
     6301 ccttgccccc gaagaaggga ctatcgaaga acaactgaaa caatgcttgg cacgccgggc
     6361 ggagctcctc gagaaggccg ctggccaggg gttgcctgcg gatcgaatca ccaacttggc
     6421 cggggccgtt ggtgctagtc ggcagctcaa gcgccaactt gccgaggcag cccatcaagc
     6481 gcgtttagtt caaattcaca atcttaccaa ttggatgctc aaccaacggg cgctcattca
     6541 cttgtcgcag ttaattgaaa taatcgcgac cggaggccgc cgccatccga catatcatta
     6601 tgacagcaat cagtctctcc cagaaacaca gggtgttttg gttgatcaca atgcctagaa
     6661 aaactgcggc ttttaggcaa atgacggata caggataaag cgatgtcgct gttcagttcc
     6721 atccgcatgg cgagcaatac gatgagggcc gatcagattg cgctccaggt gatcggccag
     6781 aacatcgcca atgccgaaac gcccggatat atccgcgagg agatcgtcct caagccggcg
     6841 cccattcaaa agctcggggg gcttcccctc gggctcggtg ttcaggtcgc cgccgtcatc
     6901 cagaagcttg atcagttctt ggaagaacgg ctgcgggcgt cggtgagcga ccgggcaggc
     6961 gccgaggtta ccgaagagac ctatcaccag ttggagggac tgatcggcga gttgagcgat
     7021 actgatctta gcagtgactt aacacgtttc ttttccagta tctccgaggt cctcaaccaa
     7081 ccggaaagtg tctcggcccg gaatctggtg gtcctccaag cacaaacgct gacgcaaaac
     7141 atcaggcggc tccatgatcg cgtcaatgcc attcgctcag acctgaacaa acgtattgac
     7201 gacgttgcgg atcgcattaa ccgattgatt gaagatatac gacggctcaa cattcgcatt
     7261 gccgagaccg aaggtggtga cgtgtcccaa agcgacgccg tggggctgcg cgaccagcga
     7321 cttcaggcac ttgaagaact ggcaagtctg atcgacatcc gggtggagga gcaaccgagt
     7381 ggcggcgttg ccgtctattc gagcggattc tttctggtgt acgaaggcac atcccgagaa
     7441 gtgacggtgg tgcggagcac agataggggc cttggggttt cggaagttcg gctggcagaa
     7501 acggattcgc cgcttgaagt tcatgccggt gaactgtacg gattgataac ggcccgggat
     7561 tccgtcctgg gtagcttctt agaccgtttg aatgagttta cggccgccct gatcaacgga
     7621 ttcaaccgca ttcacgctag tggccaggga ctcagcggct tcacacagct cacgtctgtt
     7681 gaggggattg ctgatccgac ggtgccactc aaccaagctg ggcttcgatt cacgccacag
     7741 aacgggtctt ttctcattca attgctgaat actcgcacgg gcgccattca aaccacccgg
     7801 gtacgtgtta acctggccgg cttagacaat ggtaccaccc tgttggattt ggcggatcag
     7861 attaacgcca ttgatggatt gcaggcgagc atcgattcgc ttggcaggtt gaatatccaa
     7921 tgcactgggc cagatcaagt gttttccttt gccgaagaca ctagtggtgt cctggcgagt
     7981 ttaggcttga atacgttctt cactggcaca ggtgccggcg atattggtat caatcgggtc
     8041 ctcacacagg accccagcaa gttcgcagcg agtaagaatg gcattgggat tgatacggaa
     8101 actgccgtag cccttgcagg ttttgccgat agcccgctga ccgaatatgg gggacgaagt
     8161 atcaacgccc tttacgatca gatgattagc gaggttaccc aaggggcggc aatttcgcgt
     8221 gccggagcag agtcggcacg aacatttgaa accaccttac gaggacagaa gcttgctgtg
     8281 agtggagtca atttggatga ggaggtcgtc aaattgattg catttcagca gcaattccag
     8341 gtttctgcgc gatttatcaa aacgctgaac gagcttattc agattctggt gaatatctag
     8401 cggaccattc cgacacccat acaaagcacg agagaccctc gaaacactga cattagcaaa
     8461 acgagtgggt cccacgaact atgcccagcg tcaccggtgt tccgacaaca cggattacga
     8521 acctctttgt ccgccagcgt ctcttgcagc agattcaggc ggaccagcgg gatattgtca
     8581 atcttcagct tcaattaagc acagggcagc gtttttcggt acccagcgag cagcccattg
     8641 tgtctttgcg tgtcattgga cttcagcggc tattggagca aaaagaacag gtcaaggcca
     8701 acctcgccac aacgcagtct tatctgaccg cggccgatac agcgctcagc agtatttcaa
     8761 gcatggttgc agaagccagg gcgacggcgc tgggagtgct cggcacgaca gccaccgaca
     8821 cccagcgggc agcggcagcc cttcaaatcc aacagacaat ccaacagttg ctggatgcag
     8881 gaaaccagaa gtttcgtggg cgctacctct tctcgggcag tcttaccgat ttacgcccct
     8941 ttggcgtaac gggcaacaat ttagtggctt tttatggaaa tgaaaggctg ctaccttctt
     9001 atgttgatac cgacctacta agcgaaaaca acgtcctggg cagccaaatt ttcggtgcca
     9061 tctcctcggc catcgaggga actgccgacc ttcagccgcg actccgtttt gatacgcctt
     9121 tggcccatct caacaacggg gcaggagtcg atttgggcag tattgtcatt tccgacggaa
     9181 cccgcagcgc cgtcgtggat ttgagttcag cccacaccat tggcgacgtg gccatgttaa
     9241 ttcgccgcaa ttcggcccgt attcccctca acgtcgaggt gacttcgcag gggctgaaaa
     9301 tccaactcaa tgccgcttcg ggtgatctgt cgattcgtga tgtggggaac ggcaccacgg
     9361 cccggcaact ggggatttac cgggagattg gagtcggaac ctcgccgatt atcggctcgc
     9421 ccctgaaccc ctgcctcaag cccacgacgc aactgaaaga tatcttggga agtcccgcgc
     9481 gggcggcctt gcgatttccc ggaagtgata acgatttcta ccttgaagct gactctaatg
     9541 gcgatgccct gaacggcgtc aagatccgat tcattgacga tccgagcgtc acggtcgggt
     9601 cggagatcgt ggaatacgat gcggcagcca aagagctatt gattcgcatt gacgaaaccc
     9661 atagccaggc gcgggatgtc atctcggcca tcaataacgc ctacgacgcc ggccaattgc
     9721 cgtttttcgc ctaccttgac cccactgacc aggaactcta cccggggcaa ggtcttgtct
     9781 tcccaacgcc cgccggagaa tgggccgctg tcacagaggg cggtagtggc gaggacttcg
     9841 acaagacgtc cggactgctc atcaccaacg gcggtcagac gtttgcggtg gatttcagca
     9901 aggccgcgac tattgaagac gtcctcaata cacttaacgc atcctcgtac ggcttgttgg
     9961 cagagatcga tgaatcggca aggcggatta acgtccgtac tcgaatcagt ggggccgatt
    10021 ttgccatcgg ggaaaacggc ggaaagacgg cctcgcagtt gggcctgcga accctgacgg
    10081 ccgatacccg cctcgaccaa ctcaatttcg gtcgcggagt aagtgactat cgggatcagg
    10141 gtacgacagc ccgagcaatc ttcgatccgg tgggcacaca caatcgactg attttacagg
    10201 cgcggacggc cgggcccgaa ttcaatggtt ttcggctgag tttctacgat acaggtggtc
    10261 cgccgggatc ggaaataatc acttatgaca gcgtgaataa gcaaatcgct atcgggatcg
    10321 ttcccggttc aacgactgcc aaacaaatcg tcgagctttt tgccgccaca cccggggcac
    10381 gggataattt tcaacttcgt ctcgatgatg aatcgctgga taatgatggc tctgggctac
    10441 tccaagtcgg tgaggtcctt acgtcaggcg gtagctctgg cggaattgat ttcaccatta
    10501 cgcgcgcgga cggcgttgtg atggaaatcg acctttcagg agccgtcacc gttcaggatg
    10561 tcattgaccg cattaacaat catcccaata atccgccgcg cgtgccgggc ggaacgccct
    10621 ggttgatcgc ccgcttggcc caatatggga acgggattga acttattgac gaaagcgtgg
    10681 gaacaggcgt tttaacagtc agtcggactc cctccagcac cgcagccatc gaactgggac
    10741 tggttcccgt cggggccgaa tacgcgaagg ccacaagtcc tggtacaatc ggccaggcca
    10801 atgtgcggtc atccggtgcc aataatgatc tcatctttag gacaaaatcg cccacggcgt
    10861 cgggcaacgg ctatcgaatc atctttgaag actcgggagg cgggcccgag tcattcacct
    10921 ttgatcccgc caataagatt atgcggttcg ccatccagcc cggcgtgacg acggcaaacc
    10981 gcattattga gctgttttct tcggatcctg tggccccctt ggtttttgat gcgctcctcg
    11041 accccaccga cggcaatgac ggaaccggct tcgttgaagt cacaagccca agtagtccac
    11101 ctactattgc ggggggccaa tcatcgcaat taacgggccg cgacgttaac ccgcttgaga
    11161 ccgagggaat cttcacggcc ctcatccgcc ttaaggacgc actgatccgt aacgatgttt
    11221 ggggcgccca gcgggcgatt gatctcttgg atgaagcggt gctcaatctc aattttcgtc
    11281 gggccgagtt aggcgccaaa caacagagcc ttgatattct gaacaatcgg ctggaggatg
    11341 aagatactca attgcagtcg gccctggctg ccgatttcga cgcggatttg gccgaggtga
    11401 tttcctccct cgtggccaag cagtcggcat accaggcagc actgcaggca accgcaaaga
    11461 tctttcggat gtccttgttg gattatctct aatcgttgcc tcataatctc tattggagtc
    11521 taacccaata agttccccct ctcccgctgg gagagggttg gggtgagggc aagcgaacgt
    11581 caaaatcgat cgagaaaacc ggcagcgatt cgagccaccc tcacccccga cccctctccc
    11641 gctgaacagg agaggggaga ttacgggata ggctcctggt cgttcatcaa gatcgatcgg
    11701 gaccgtggta accaaagaaa ctctttgtat caatgagcaa tgatcaagaa aacctcgccc
    11761 gggcgaagca ggaacttcgt caacgtttga aggccgttcg ccagtctctc gatccggaat
    11821 gggtccttca ggcctcacag gcgattcagg aaacgctggt tcaactgccg gaatatcaat
    11881 cgtctcggtc catcatgagc tacgtcagca ccggcaaaga ggtcgagacg caccgcctct
    11941 tactgcgaat gaagagggag agcaaggttg tggttattcc gtggtgcgag ggcgacgagc
    12001 taaggctctt tcgctgggaa tcgttcgatg agcttgatcg cggcacactc aatattctcg
    12061 agccccggcc tgacttacga tccaaggaga ccaaaatctt tagtccgcac gaactcgatc
    12121 tcgtcctggt acccggactg gcgtttgacc gaaacgggaa ccgcctggga cgtggtcgcg
    12181 gctattatga tcgctttttg aagacgctcc cccgaagggt agtgcggatc ggtttggcct
    12241 tcgagtgcca attggtggaa cgtgtgcctg tgtcaccaaa cgatgagaag gtggatctca
    12301 tattgacaga gttccgaacg tatcgcgtgc cccgcattga ctgacctcgg gttgtggatg
    12361 tcttccccaa cctaacttgt cttacacaat gcgcttaatg ggaaattctc gcccgcaacc
    12421 tgaccgatca gttactccgg caccgctccg cgatcgcatt gcggaaatgg gcactttccg
    12481 ggttaggaaa acgctgacac aattcgtcgg cgatggccaa tgccgcggca catcgcttta
    12541 attccaccaa acacagggca tacccttgaa gcaggtccgc cgacggattc ccgagtcggt
    12601 aggccttctc atagtactcc gccgattcgc ttgctctgcc ggccttttgc aaggcaaccg
    12661 ccgtgttgta gagcacccgt gattcattcg ggagcagccg ggccgcctcc cggagctcct
    12721 cgatggcatc ggccaagcgc gtgggaagtt ccgccaggag taaccccaag gcgaaatgaa
    12781 tatcgccgcg attggggtat ttggaaagaa tcgttcggaa ctcccgctcc gcttcgtcaa
    12841 accgtcccat ttgatagtac aaacgagcga gattgtctcg aacgggaaca aaatctggat
    12901 tgattttcaa ggctcggcga tattgctctt ctgcctcggc aaaccgttgg gttcgtgcgt
    12961 agatcatccc caaattgttt ctggccggaa ggaaaagggg atcgatccgc agggcatcga
    13021 ggtacttttg ttcctgctcc tgaaaatctc cgagaaactc ggccaggatg gccaaattca
    13081 tatgggcacc cggctgatcg gaaacaagaa gctggcttgt tcggtattcc tcaatggccg
    13141 attgcagggc tttcatagca ctgccgctca gcgcctgcgg aggaagattc aacgcccagg
    13201 ggaccgactc gacgcgcacc gccctgatgg gatcactcaa gagatgctga acacggggaa
    13261 catcgtcctc ccggatcacc acgttttgca gggcccgcgc agcagccaag cgaacaagag
    13321 gttcggggtc tcgtgctgca gcaaacacgg ctccccgggc ggcttcactt ccataattag
    13381 ccagcaaaag cacggcactg gcccgcacaa tggcagagag gtcctgccgc cgtgccaccg
    13441 ccagcaactc gatcacgccc gaggaatcaa gccgtcgccc tttctcgaag gcgtaagcga
    13501 aatgggacgg ttccttgcgg ggaccatacc atttgttgac ccattccaca gcccaatcgg
    13561 gagtttcgcc tttttcggga tcttgatgac aaagattaca agcgttcgga atgccgagac
    13621 tgaccgtaag gtccggtcga gggatgcgaa gactatgatc gcgccgtgcg tccacaacca
    13681 tgtaagtcgt ctccggcatg tgacactcca cacagtgtgt gcccggcttg gtgctatcgg
    13741 ggtgatgatg atgctggacc gtatcgtatt tactgggcaa gtggcattgt ccgcacaatt
    13801 tgttatccgc atatggctga cgaacctcac ccaccttagc cttttcggca aacttcaccc
    13861 gggccgtgtg ggggtcatga caatcggcac agcggactcc tttccgatac ataagacttt
    13921 gtagaaacga agcgtactca tagtcctcat ctaggatttg accatccgga tagtacaaat
    13981 taccatcgag gagttccggc acgtagtagt tgaggaaagc ctctcccggt ggaaacgggg
    14041 aagcaatggg gcgacgccgc gcatgacatg gggcacaact atcgacaacc gtcttgttgt
    14101 cgcacttctg tggcgaaaag ctgaccagcc cgttgccgcg tttccggtcc caaaacagac
    14161 tccacgagtc ggccaactgg acatggagac tggccggacc atgacatgct tcgcagctta
    14221 cgtcgatctc ggaccattcc gtgcgataag tgttggtcgt tacgtcataa tttttctgca
    14281 aattcgttgt gtggcagtcg gcgcacatgt agttccagtt ttgcaaggcc cgcgtccaat
    14341 gcagcggatc ttgcgccgga atggggtcgc tcggatagag gtgaaaccat cgtcgggact
    14401 tcacgtccca ggccagcggc aagcactgga cacgtccgtc tggaaatgtg accagatact
    14461 gctgcaacgg gtaaactcca aaaacataat ccacctggaa ctcgcggggc gtgccgtccc
    14521 ggtcatcggt gagaacgtag aatttcccgt ttcttcggaa aaagcggctc gtaacggccc
    14581 aatccgggtt ctcgatttgt ccgctctgga tgagatcccg ggcggcgtca cccagcttgc
    14641 ggtgggcatc ggtgatgtca catggtcgag caaacgtctt tttccacgtg acaagacgct
    14701 caacggcgga tcgacgttct tccggtaaga cctccagaat acgctgccgt gagcggtcac
    14761 cactgggcgg ggtataaggg tagaacgtct ccggcgtgtg ggcaacttcg taaggttgaa
    14821 gggctaccgc tagcgtctcg tcatcaagcc gcgagaggat ttttctcagc tcttcatcag
    14881 aaaaccgcca cacgtcgctg aaggcaatat gggtaaatgt acaatcgtca aaattgccga
    14941 ggacggtttc cgaagtggcc acatccatgg cgagatcgtg atcagaccct tcccaaaggc
    15001 ggcactcttg ttcatggcag cggtagcagc tttcccggcc cacgtatgtg acggagaccg
    15061 agtccggcac cgtaatccac caatcgagaa acaccactaa cgccaccccg acaagaacaa
    15121 tggccacaaa gaggaaccat ctgcgcggcg aacgctccct cagacaaaag ccggtaacgt
    15181 tggcgatgag gtgccgagtg acctgaaaaa cgcggagggc gccactagaa acaaggctag
    15241 ggccagaggc gaggtcgcga tgttccggag tgttcttgca acgctgctta ctcacaaggc
    15301 acatcacagg aaaaataacg attacgccgc atcggtggcg gggttgtctt tcgtgacctc
    15361 aaacacgcag caaggagccc cgtcatggcg acacgccact tgccgcaccg gccctccgag
    15421 aaccaacgcg aacgtctctt ggtcaaggag gcaagcagcc cgattctctt cgtacatcat
    15481 gatgtatggg caactccgct gacgaatggt ataacggcca ttttcctcct gaatatccac
    15541 caggacccct tcctcccgaa gaatctcgac taactgtaaa acgcgttcgg ccggcgttgt
    15601 cgctgtaatt tgcttgcggt aatgctcggc cagccatcgg ctaacctgtt tgatgacttc
    15661 gcccaggagc tttggcccac ccacttcgcg gatcgcccgc caaatgttgg gcaaaaccat
    15721 ttcctgattt ttcgagagga ggaggagcaa ggcacctggg gtcgcggagt aaacgtggcg
    15781 gggtcgtcct cgaccaggaa ggcgttgcat ctgccgctcc accaggccgg cggctaccag
    15841 ttcattgagt tgctcggtaa cggctgtccg cgtgactttc atctcgcgga tgaggtcggc
    15901 cacactcttg ggtgtgttcc cgaccagcaa cttcactacc cgcatactcg caggacttaa
    15961 attcaaattc accatctgcg ctgtccgcca catatcaagg accccgaaaa aacacccgtt
    16021 tcgaaccgcc cgcatagggg atattatccc cgcccggtgg accacagaca ccaaattgtg
    16081 ttggccaaat tggcgatatt gtcaaaaacc gacactccca cttgcgagtc gcaaccttca
    16141 atcgcagggc cttgtcgtgg gcagaaataa agacaaaatc gaccctgctc cgtgcgaccg
    16201 ttaagtgcat catcccgccg ccccggaaac aggtgtcgac gcctttcatt gtcgcccagt
    16261 tcttggggcc aagtcaaggt agcaagacac gaaaggcaca aaatcatgtg acatctccgt
    16321 tttaacaaag gctacctcgg agagattctc tcgtttcttc gcatccgccc agccgccact
    16381 gtgttcagga ctatgaaaat acggctcacg attctgttta tcaccagcgc tttgtcatga
    16441 aaagccaccg gtagtttcaa gcggaggtga ccgattagcc aggtcccaaa gtggacccct
    16501 tccctttgta accttcctag gacggaaggc atcagaccct ttgagggttc gtgatcagga
    16561 tgtcacgcca aagtcaaata agtgttacat gtcatggagg tttgaccttg aagcggaatc
    16621 agatttggca aaagtcaggt tacctcacac gttatgaggt actttccgtg aagttacgga
    16681 ctgttggggc attcgtcctc gccggaggac ttaccattct cggaggatgc accagtgagg
    16741 ccctttccgg ctcgcatctt cgccccgaat cgatttccga aaccctcgct gttactccgg
    16801 cggatcagaa cagcgtgagg aggtccggcg agggcgacgc gatctcgagg gaaacaacca
    16861 aaactgatca gccgtttcgt gatacttcaa caaatccaag tcaagagaaa aaggagacaa
    16921 gaacaatgtg gtttaccggt ggaaaaaaca ccatcgagca cgccaacgca gcaaatttca
    16981 acacccttgt ggcccaatcc agtgtccctg tgttggttga cttttacgcg gactggtgcg
    17041 gtccctgccg tgccctggca ccaattctcg agcaattggc ccaggaagtt aacggcgttc
    17101 ggatcgtcaa agtgaatgtt gatcaggccc ccaccttggc ggcccagtat cgtgtctcgg
    17161 ccattcccac gctcatcctc ttcgatcaag gaaggccggt tcaacgtatc actggtttgg
    17221 ctagcaagga ggatttgaag cggcttttgg ccctcgattg acgaccaaga tccgcagttc
    17281 tgtagctgta gttctgtagc ctgcggacgt gcgggggcta gtcgggaatc gtattgttgg
    17341 ggttttaaga tcattcatcg catcctgtgt tgcgacctcc gcttatcgcg gaggtcgttt
    17401 tgatgcgctt caaaacagcg tctgcgagaa gtccccacag agccactggc aacccgccat
    17461 ttggttgcgt ttcccacagt ccacaattgt gagacgctcc tgttgcagcg gaacgcgtag
    17521 gacggtagtc ttatccaaat gatttgccga gtgtttgttc cagatagctc atctgtgcaa
    17581 ccgtggtgat gtgataaagg agccttaagc atggccaaat cggcagcacg ccagtttgcg
    17641 atcggtcttg attatggcac caacagtgtt cgggcgctga ttgtggatgt gcgaaccggc
    17701 gaagaggtcg gaaccgcagt ttatcaatac cccagcggtg acaaaggcgt tctcctcgat
    17761 ccgaagaatc cgaatctcgc gcggcaaaac ccggcggact atattgaggg gttttatcgc
    17821 tgcgtacggg cggccatccg cagtgtgccg aagagtgtgg gattcgctcc agagcatgtg
    17881 gtgggcatcg gggttgatac gaccggttct acgcccattc cggttgatga aaatggctgg
    17941 ccgttggcct ttcaaaaacg ctttgcgaat aactttgccg cccaggcctg gttgtggaag
    18001 gatcatacgt cctatgccga ggccgcagag atcaccgaga aggctcgggc aatggggctt
    18061 ccgtatctcg cgaaatgcgg cgggacttac agctcggagt ggttctggtc gaagatattg
    18121 cattgtctcc gaaccaatcc aaacgtcttt gatgcggcct actcctgggt ggaattggcg
    18181 gactttatcc ccggcttttt gacagggaat cttaagccgg agaccattcc acgctcgatc
    18241 tgtgccgccg gacacaaggc gatgtacaac ccagaatggg gaggcctgcc cagtgaggaa
    18301 ttcttgcgtg ccctcgatcc caagctggtg aaaattcggc agcgatatgc gccggaggca
    18361 ctaccggccg atcgtaaggc aggtgagcta acgcgggagg tcgcccgcaa ggttggtttg
    18421 cggcccggaa cgccggtggc cgtcggggcc tttgatgcgc acatgggagc ggttggcgcc
    18481 gggatcaaac cgggaaccct cgtcaagatt atcggtacta gtacctgcga catgatggtt
    18541 gtgccgatga atcagccact cccggatatt cctgggctgt gcggaattgt gccgcactcg
    18601 attattccag ggatgtacgg gctggaggcc gggcaatcgg ctgtgggaga catcttcaac
    18661 tggttcgtaa gttttctggc accggctaag tacaccgccg cgggcgatcc ccacgcagca
    18721 ttgactcgtg atgccgagaa attgaaaccc ggcgagagcg ggctcctggc cttggactgg
    18781 aacaatggca accggacaat cctcgtcgat cctctgctca cgggattgct cgtcggtcaa
    18841 acgctgttga ccacggcccc ggaagtctat cgtgccctca tcgaggcaac cgcctttggg
    18901 gccttaacga tcatcaagag atttgaggaa tatggtgtga ctgtcaaaga ggtcgtcaac
    18961 tgcggaggca tcgccgagaa aaaccccttc gtcatgcaga tttatgcgga tgtctgcaat
    19021 cgaccgatga agatcagccg atcggcgcag acgtgtgcct tgggggcagc cattttcggt
    19081 gctgttgcag gcggggcata taaagatgtt gaacaagccc aacgtcgcat gacgggcacc
    19141 aagccgaccg tctttcgacc acgaaaacaa gcggtggctg tttatgaacg cctttatcaa
    19201 ttgtaccgtc ggcttcatga cgcctttggc ttgcccgatt accgcgattc cctgtacgat
    19261 gtcatgaaac agctcattgc cttgcgaaaa gaagtccgcg gcgaatcgtg aaccaggtgt
    19321 aggcgtcgca tggtaagccg caggttgtct gcgtaacaca gttgccgtgt tgcgccggca
    19381 gcagtgggca gcttttcccc ctgatcagcg ggagtcacct gagatgagtt tagaacagtt
    19441 gagagaagag gtttgtcgtg ccaacaagct tctgatggag cacggtctgg tctgcctgca
    19501 ttcgggaaat gttagcggac tggatcgcag caccggccga ctggtcatca agccttccgg
    19561 cgttgactat gagacactct ctccggaaga cctcgtcgag gtggatttga ttaccggcaa
    19621 ggtcatcagt acccatctgc ggccgagcgt ggatttgccg caccatttat atatttaccg
    19681 gaatatgccg ggggtcggag gtattgctca tgcccatagt gactacgcca cggcttgggc
    19741 ggccgtggga cggccggtcc ctctttgctt gacatccatg gccgactatt tcggaggtga
    19801 aatcccttgt gctccgtacg tggataatga gggcgacaac atcggccagg ccatcgttcg
    19861 ttatcgggga cgtgcccccg ccatcctctt ggccaatcac ggggctttca cttggggacc
    19921 gaccgcgctg gacgctgtca aactggccgt ggcgctagag gatgcagcca agaaagcgtt
    19981 tttggcctct caactgggcc aacccctgga aatcccattg gaagaggcgc gaaaatggta
    20041 cgacaagtat catcacggat acgggcaaat cgtaccccca cgggcgcccc aaacggtcac
    20101 tcggcaacca cagagccccg gctgaacaca ctctcgaccg gtcaccgcgg ccaacgacca
    20161 cagacattcc gcttattcat gcgtcttaca tcatgcccgc cagggccgct gtcggttttt
    20221 caactcggcg agaatcttgt tcaccaaggc cacatccgtg gcgatctcga aatcaaacat
    20281 ccccgctagt acgaaatccg caccgttttc aaaggcatag cggaaggcca cttcaggcgg
    20341 aatggcgccg gcggccatga ccttgaaggc aaaccagggc ttctccacgg actgcataag
    20401 ggcagccaat tcctccggtt tggagcacca ccgcgaatcg taatagagtt tggcgctggg
    20461 atagttctgc cggtggaatg tgacgacata gaagtcgcaa tcgagtttcg ccttctcgca
    20521 atcctccacg actcccatac ggtgtgcccc aattcccaca gggagcttgt gcgatttggc
    20581 gtagtcgagc gcttgagcca gcagttccat ccgcggtgct cccacacgct cgtcggcctc
    20641 cgggccacaa acgtcgttaa ggaagccaca ggagaagtcg ccctccaacc cagaaatgta
    20701 aagtgcattc gtacccgcag ccacgagggc atcgatttga cgtttgaatt catcgagtcc
    20761 cccgctgacc aaggcgtgag ccgtgcacgt gatccactgc atctttccgc cgcgcttgcg
    20821 gtgctcctgc aggatactga gagcgtgagg gacataatgg ataaacaggg tgttaatccc
    20881 gtgagcctcg gccacggcaa gtgtttccag gattttttcg tccgtgttgt aatgtcgggc
    20941 caaagtatat acgtaacgca aatcgcggct atgggtgaaa tgcgtcaaaa gattacctcc
    21001 caaaataagc cggctgatct ccaggtcttt gattttgcct tttggcagcg atgtcaccgg
    21061 ggcgcttgcg ggttcctccg ccaccgtcat ccttgtcaat gcctggcagg ataaagcccc
    21121 cacggtggag ccaaagagtg tcgttccgac aaactggcga cgcgaaacat gcttttccac
    21181 ggttaaacct ccctattgtt ggcgatggat acggcgagca acacacactt tttccggcac
    21241 ttttccccac agtcgatcag acaactcagt ctcggttatc tgtttccttc ttgccgtttt
    21301 ctaggtctta cctcacagaa gcgatcggag aaacttgacg ctggcctcca tgaactcctg
    21361 tgtcccgtag cattcgatgg acacggcacc actccaattg gtctctttga gatattggat
    21421 cacccgccgg atgttctccg cattaactcc cccgccaacc gggacaaagg atgtcccgat
    21481 tcctgtctct tgcccacgag cggcagccgc caatgattcg ctcacgtcct tgatgtgaca
    21541 atgaacgagg tagggtcgga actccttgca aaactccagc ggatcatgac cggcaatgaa
    21601 cgtgttgccg gtgtccagat taaaacgtag gtattctgac tgaaaataat cgaacagacg
    21661 atgcaaaaac tccgggtcat ttgtgtaagg accgtggggt tcaacggcca ggatgatccc
    21721 ataatcctcg gcccaactta ggcactgacg gtaattatcg cacgtaattc ggaaaacctc
    21781 atcgcgactg agtcccggaa cctcgacggc cccgtccgtg gtatccacga tcggacagtt
    21841 tagttccgcc gcaaagcgga ttgtctgctg gacgtagtgc acaccaaagg cggagccttt
    21901 tggccccatc aaaggatagg caccgtcaat ttgagaaacg gatagtccct tcgattctag
    21961 gtaacggcgt aaggcccgcg gattactttg aagtgaaact gccggatcgt agcccatctc
    22021 ttggatgaaa tcttgtccgt tgatgacggc gaactccaca tgtttgaggc cgagcgcagc
    22081 ccccatttcc accgcgtact cgaagctctt gctgagggga cgccagttat ccgtgtgaaa
    22141 tcccagttcg atcatttgaa accctcccgt ttcgcaaagg ccgcaacgca gcaccacatc
    22201 ccaacaaatc ttgcccatcc cgctgacctg aaacgtggcg ttcacgaagt ccaggccctt
    22261 acattgtcgg catcggagtc gttgttgaca agcgaaagag cgacacaagt cgaatgatcc
    22321 tccatgcaag gtcgcggatg cgatctgaga agatcgacca cgtggcatac ggcgggcttc
    22381 cgggcctcaa agttacggct cctcgtgatc tcgcgcctat ccccggaggg cacatgatcg
    22441 cctctcattg aacctccgcc gtctcgcacc gcttgcctgc cgcattcgct ggcagcaccg
    22501 tcgcttccgc atttagcgat ccacttgcgc cgaggagcta tccagccttc cccgattgat
    22561 ctgatgatcg cagcctcagc gggtcttttc tggtgattcg cccgacgctt cgacgggttt
    22621 catcccgaat ccctcacgga cccaattgac cacagcttga ccggtaaacc gagcatccca
    22681 cggatagtcg cctgtccagg agggcttgcc cgacgtggga atcggatcgg tgccgccccg
    22741 gcgcatgttg gtggccacag ccatgaacgt tttcaagccg gtaggatcat agggttcacc
    22801 ggccttgacc cgtctggcca cctcgtcgcg atcgagccag ggatcgtctg accaatccaa
    22861 cagcgtgagc ttgagcactt cttgcacata tcgtttgagc gtgcggtcag ggtccggcca
    22921 tttctccctt gcagccgctt cgtcgcgggg agcaactgtg ttatctgttc ccatccagac
    22981 cgcctgggga agatcgtcgt tggcaacgta aaagcgattg tttcgcacaa ccaactggtt
    23041 ggcggaggtc aggtgctcgg catcgctact tacgattccc tcggcaacaa acacgttgtt
    23101 ttcgaccacc aaattcatcg tgtcggtcca atcgccggtc aggcgaaggc caaattttgt
    23161 ccggtagcca atattccctc gaatggtaca acgttgcatg gtgtaatagt tgccatcttc
    23221 atactggccg cgccccggcg ataggctgaa agcagcctga ccgacgggcc gggagaaacc
    23281 ggggtcatcc gtgagcatcg cgccgctcac aatattgttt tcgactacac caccaaacga
    23341 tgccccttgc attgtgtaac cgtggccggg ctgcgcccgc cggtccgatt ttccatcggt
    23401 gtcagggtcg gcaggactgg gataaacgta cacaaactgc acgttgttgc gaacgattgc
    23461 cgagcaaccc gtctgcttgc cagcggtcag ccaggggtta aagagacggt tgctggcggt
    23521 agaactgaac cagtagccct cgatgatcaa actgttctca cataaccctc ctagtcgcat
    23581 ctggggacca cctgagccgc catcggccga gatgatgttg cggtaggtat gccccatctg
    23641 cgctccacca ccctgataga tgttgcggtc gaagatggtg cgtctgggat cagggtctat
    23701 tctgggatca tccttat
//
LOCUS       DAOSUT010000060        23471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1417_length_23471_cov_10.153698, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000060 DAOSUT010000000
VERSION     DAOSUT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 23471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23471
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_23471_cov_10.153698"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1019)
                     /gene="fmt"
                     /locus_tag="PLD05_07275"
     CDS             complement(57..1019)
                     /gene="fmt"
                     /locus_tag="PLD05_07275"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOP77275.1"
                     /translation="MRLVMVGTGPFGVPTFEELYNTHHSVVCLVTAPLVRHGRPVPPS
                     PLRAIAAAHGTPIYDPEDINAPEARQYLEGLDADLLIVCDYGQILAPETLATARLGGI
                     NLHASLLPKYRGAAPINWAIYHGEKTTGVSVIHMTPQVDAGPIIAQGEVEIGPDETAP
                     EVEAKLAKIGAWFVRRALDNLEAGLLEALPQDPKLASKAPRLKKSDGLVDWTRPAEAI
                     RNQVRAFEPWPKTYTYWHRPGKPPLQLILGPVSVTHDVPPGVVPGTVVSAGKSGIVVA
                     AGEGGVVITQLQPAGRRMMSADEFIRGYHLQVGDRFGADASASK"
     gene            1559..2881
                     /locus_tag="PLD05_07280"
     CDS             1559..2881
                     /locus_tag="PLD05_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOP77276.1"
                     /translation="MVTQAKRLLLVLVAAATVLMATGCPKPATEQKPTRPSTTAGLEM
                     ETPSERPQAPVKEESAEPTAPSQTEPAAEPGQMAPTTEPAAEEKAAEKTEEDTKEKAT
                     EGAAAPEMPQTTPAAESQAQAVPQGTPFEVKVPLGLPPLPIPEDNPMTVEKIELGKML
                     YFDKRVSADGTVSCATCHDPKMAWAEHLPTSKGIHEQVGERNAPTVINAAYAPSQFWD
                     GRAKTLEEQAVGPVANPIEMGHTLEGMVQSLDNIPQYQELFKKVFGTGVTAENFAKAV
                     AAFERTILSGNSPYDRYKAGDQNALTEAQKRGLKIFEESGCADCHAPPLFSTYEFYNA
                     GIGMNKEKPDEGRKSVTGKEEDMGAFRVPSLREVANTAPYFHDGSVATLEEAVAIMAK
                     GGIDNPHRSAEFDTVREANITAEQQKDLVEFLKALSGEFPVIEPPQLP"
     gene            complement(2902..3399)
                     /locus_tag="PLD05_07285"
     CDS             complement(2902..3399)
                     /locus_tag="PLD05_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOP77277.1"
                     /translation="MTVTASSSSLGSKIINVGMGQTAVGDKEAVFTSVLGSCIGIVLW
                     HPHLGMGAIAHVVLPDSHGQNGQPGKFADTAVPYLIRQLADIGCPRTGLVAKIAGGAC
                     MFRGGGPLQIGDDNIAATRTILRQHGIAITAEDVGGTSGRKITFECATGRLVVEVAGQ
                     TVRVL"
     gene            complement(3492..4118)
                     /locus_tag="PLD05_07290"
     CDS             complement(3492..4118)
                     /locus_tag="PLD05_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOP77278.1"
                     /translation="MNPTTIHRVEVLRPLFAAATHEASAAMCRWTDGVISLTLDDICE
                     LPLEEVTAELGFSDDFMTMVVLGIESEPGTVLILTFDEENARQLAATLTRRQRNTTPE
                     WDELEISALTETGNILGCAYVNALTRLTGQILIPSPPYFVRDYFASVLEQALVEQAMS
                     SDTALLCRTGFHREGENLNWHVLFIPSKSLREKLERSVASVSHDRARR"
     gene            complement(4219..7155)
                     /locus_tag="PLD05_07295"
     CDS             complement(4219..7155)
                     /locus_tag="PLD05_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOP77279.1"
                     /translation="MDAYVSDDMYQEMLADFLDESTQLLDRLGENLLTLDEWVKNHAP
                     ESREQFDAEILNEMFRAAHSLKGLSAMLNLSNINQLTHKLENLFDAVRKGQMVVDDEA
                     VELMFQATDRLRAMVDLLRNQNSDAIDAQDVVTAIAAMLEGRQPGPSVAETRAMEALS
                     QAETALAEAMSDSAPDSSAVSQASPLPTDNARFQEEAAARFQDVHDEADLSSGYIALF
                     IDELAQGLDVLTETLLEAEGQGTSTQLENLLCTAHRIKGSAASVGFHRASKLAHLMED
                     ILQGLRETGRPLTRELADILLKCTDGFRSFLEQLRQGQSQYDADFVSLAADLLACASS
                     LKNPPAAAVSLTVPTPSPRETNQPLGVWPSAELLQTARNLTSGPSLVGRVEFQTNLDL
                     SGLKARLIYEKLCHQGNVVWFSVPSEQLDQIEKLDYVEFIIETSQPEESITRAIRVGG
                     VKQIHIQPLSKDSGAVNYKAGSSAKPSEMVPKQPVAEGKEKPTVSDGGGDQEKSRPSG
                     EAVKAVETLRVDIERLDQLMNLAGQLVITRARLTELNDKLKGFFQSNRASQVLRGLRD
                     LVGRIAGMSSLGSSSGGTSRLESTLADRLEKEFEALAHELEALDQARSTVNYLAEAVH
                     QLDRVTDGIQRAVMETRMVPIGPLFNRFKRVVRDVTRLNGKNIQLVINGEKTELDKRM
                     IDELGDPLIHLVRNAADHGIESPEVRRQLGKPEQGTITLNAFHRGNSIIIQVSDDGKG
                     LDTERILKKALERNLITPGQAERMSRQEIFQLIFLPGLSTAEKVTEVSGRGMGMDIVK
                     AKIEELSGTIDLDSVPGSGTTVTIKLPLTLAILPSLLVQVDGDVFAMPIEAVNEIVAV
                     PSEQLLTVHGLRTARIRERVISVVELSEIFGGTLSRSQIDQEEHREMTLVILGDADHE
                     IGMVVDRVLGEEDVVIKSIAENYRNIPGIAGASILGDGRVSLILDVAAIMGMITRRVG
                     SHAMSS"
     gene            7541..8806
                     /locus_tag="PLD05_07300"
     CDS             7541..8806
                     /locus_tag="PLD05_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOP77280.1"
                     /translation="MSDSERTQEVRGPIEPTETGQGFRIPVADRVNRLPPYLFARINR
                     LLYQKRRAGADVIDLGMGNPSDSPEDIVIEKLASAARDPDNHGYTESRGILNLRREVA
                     SKYFKQYGVRLDPESEIIVCLGSKDGFSHLCLALLGPGDTAIVPAPSYPPHVYAVILA
                     GGNVISLDVTDPERYLSHLEFTCRNLFPRPKLVVVNFPHNPTTTVVEPQFYHDLVKLA
                     KRFGFMIVSDLAYADVTFDGYRAPSLLAVPGAFDVAVELTTMSKGYNMAGWRVGFCAG
                     NSEMIRALATVKTYYDYGMFRPIQIAAIVALRHTDAAVEAQAKEYQRRRDVLCEGLER
                     LGWPIQRPKATMFVWAKIPEPWASQMSSFDFAMKLLKEADVAVSPGGGFGPAGEGYLR
                     LALVENEQRLKQAVRQIGRCLARAGATST"
     gene            complement(8987..9175)
                     /locus_tag="PLD05_07305"
     CDS             complement(8987..9175)
                     /locus_tag="PLD05_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77281.1"
                     /translation="MLTEQQVAKSWYSLFAKGPINEKTIERAESLLRHLRPESPLHYR
                     LSKELEEIRARLMQNAKA"
     assembly_gap    9411..9510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9802..10980
                     /locus_tag="PLD05_07310"
     CDS             9802..10980
                     /locus_tag="PLD05_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOP77282.1"
                     /translation="MADWHRSQFVGAQPHCGPNRLTGLITKQQRLTSGTLWSAVLSLT
                     VCLLSFVGCAERNVYRATRLPLEYQAPIVQDLREIPLTQLVAQQSPSDVIASGDVLEI
                     TINTSLNTKASPVTCRVDDQGMIFVPDIGPVSVGGRRLSDAERLIAETAIARDVYRQP
                     IVNVVRKEGRTYRVTVTGAVEKPGVYHLPASQADLLTALVQAGGLTKQAEPTVEIRRG
                     QNITEVSVPLENPAENAVVEGRLTSFEESTTNTVRIDLISVSSSPASDRRYQLFDGDV
                     IYVPERKVDPIFVDGLVTKPGMYEWPNNQEVRLLDALAMAGGRNNPLADNVWILRHVP
                     GREEPIVIKAKLNAIKRNAAENIRIAPGDVITVEETPGTFLYSLLKGFVHLGGSIPLN
                     "
     gene            11050..13353
                     /locus_tag="PLD05_07315"
     CDS             11050..13353
                     /locus_tag="PLD05_07315"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOP77283.1"
                     /translation="MNDFSLASSAVPESTTHLLQVAVQFISAVRRRWHIVATSVLATG
                     ILGALYYATATRYYTSKAEVLILQAGDNPLSPTIQSQNNRENNLMPTFVNLMTSQKVL
                     EGAIRQLSPEDCLVDLNPDKPERWPAQLRAGLTVTSVGRSNIIAVEYKSRHPEVAARV
                     VNAVVQSYLDFMRTTTQGTAAEIVHVLNTEKDQIAAQLSEKEQALQQLSQQIGALDTG
                     VNSQAIHPLVERARAFNNALVEAQKTRVNLEATIQAVEAALRNGQDIREYLFSGSDTV
                     GQQLLLGLLGFDREESIARANMERQLLEDRVQLANVQKYLGNNHPQVQLLQQRIAATE
                     DYLRNYQRRIEARLAMLGDSELTSLLLGTLRHRLQESVQKEHALQLQYEAARQQAATL
                     AGGLAQMEMLKNDIAGLRELYSGLVRRITNMDLTGDLRAALVTEPVANPTPVSPNLFR
                     VISMVLLGGLMLGFMAVYVMDVLDDRFRSIEEMQWQLRAPVLSIVREMPPRSGQGLEA
                     VEVAADPDSIASESFRTLRTALALSDAPTNSVVITSVQPGDGKSTVAANLAVAFAQAG
                     KRTLLIDGDMRRAGLSTFLGFKGQQGLSEILRETAAIEESFVSRVRASLAANLDLLPC
                     GSRPANPAELLSSQRLTELLQWATSRYEQVIIDTPPASLASDTAIWGRSAHGVVFVLQ
                     PDKDKRRAVLRAVEYLAILKIPLLGLVVNRVKSQSEKEYAGYAGEYYGEESNPSDETA
                     DTPEAQTTDAVPSNEHHHRRIVPRKVA"
     gene            13438..16257
                     /locus_tag="PLD05_07320"
     CDS             13438..16257
                     /locus_tag="PLD05_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOP77284.1"
                     /translation="MSDAVFLVGLWGALLLFGGRHPWGHAVLIVVSLAAVALTMTQRM
                     ILKSWTTRPLGLGWLALAGVSIPLLQLIPLPENWLSFLSPQLRRILPAWNDPTTASTL
                     GAWRCVSLAPAETQTALSLWLAYCLVFFYVIQRIEDIEDVESVLRWFVPIVLVLSIFG
                     LLQYLFSNDKFFWLYEHPFAKTTDALKASFTNRNHFADFVALTAGLLLWWSLADRRPH
                     GGSRRGPTALCEHQSLEGDDTHKYLRCFSLPLVFLALLLTQSRGGTLAAVIALVIAGV
                     TLFRTGWLKAQWLGAFLLGLVFLMTAVLIVGTDTIAGRWEGLVNLSWEQLDRGEARRT
                     VWQNTWRASQDFWPLGSGCGSFSVVYPLYQPSRSTSLYYTHAENGYLQVLLEAGLPGV
                     FLIALTCATLVIWGWRAWRAAPTPRHRAAVGAVGAGLAAFAFHGLVDYVWYVPGLAAV
                     VAILIACLVRLYQMTQLAAQHRTASATMSLASPSSSLTLRWTSFTVAFILCAAWQIPA
                     AQRSFFAGIAEFSWHRFLALYRTADWSDLRGAIQSTVASNVVSPGDEELMDSENPGLS
                     ASDAPAASTATTMGFQGEGPTQLATEREMIDLLLKAIHRDPRRAEAHLRLAKAHLRMF
                     HLIQEQNENPMPLSQIRDAVFLSQFSSQEELDRWLQTAIGNHVTLLHSAKQEALSAVR
                     LCPLLGEGYFILGQLSFLNGGREEDVMAWFRQSLAVRPNDGELLFRVGAELALAGQYQ
                     EALELWRRAFRCGRLYQIRLLERLMGSVPPGEIDQEITFLIETFEPDLEMLRYMYRQY
                     RKRYPVASLDPLRRAYAVALTSQLRDERNPPSQRAELWLELHFLHLDAGEIQAALQCA
                     EEAVRCNPLDYRAHHYLSLRLEEMGRFAEAEEHVRWCLQRRPGHRSLQNRLHNLYQKR
                     MKAEDEARTWRMAEAVSPGGYPPNR"
     gene            16922..18835
                     /locus_tag="PLD05_07325"
     CDS             16922..18835
                     /locus_tag="PLD05_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOP77285.1"
                     /translation="MSWIRRPFREQWRGAIRDLMGLAWLSLAFLVSYFVSYYVRFDGD
                     SWLWINKWFLNTVAYVCAVKLLVFWGFRLHRSWGRFLTFHDLVLLVEATTVALAAVAL
                     VDRLLLPPRMIPRGILLIDWGTTLLVLGGCRAAIRAVWDGGWHNFFTNASPVLIAGAD
                     ESGEELLRAISRYQATRHRVVGFLDRDSRRLGNRIGGIPVVGTFDQACQVATRLGVKE
                     ILLAGGALTGKEVRRLMEEARRASIEVKVLPSYEQLLAGRIAVQPRPIAIEDLLRREP
                     VTLEWKNIHRWLEGRTLLVTGSAGSIGAEIARQLLHYSPRCLVLLDRSETGQFFLERE
                     LKEIQHRLLANQKSVPELAVVLADVLDLPRMQSIFHQYRPEVVFHAAAYKHVPLMESH
                     PGEAFKNIVLATKNVADLAARFGAESFVLISTDKAVNPTSVMGACKRLAEMYVQSMTN
                     TTSTRFVTVRFGNVLDSAGSVVQIFRQQIAEGGPVTITDPRMERFFMTIPEAAGLVIQ
                     AGVIGESGQILVLDMGDPVKIIDLAMDMIRLSGLEVGRDIEIRVTGLRPGEKLFEELR
                     AAGERTVPTRHKKIRVALGSRMDRATLVRAIEQLRDWADSDPPRVIAELKRIVPGYGV
                     SQMPVPEHIRRAA"
     gene            18984..20108
                     /locus_tag="PLD05_07330"
     CDS             18984..20108
                     /locus_tag="PLD05_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOP77286.1"
                     /translation="MKSQRHSLDKSIRYPRVVFIVGTGRSGSTVLDLFLGQHPTIQSA
                     GELCNVVKSGWVGGEFCSCGERVPLCRFWTEVRKIWEELTGAKPEDYESLRSLVEGNR
                     RTLWKMLRNDRPEMLLLSGKEPGEFLAYAQWTAALYRAIAEVSGKTLLIDSSKNPARA
                     LALVLMDHRLDLIDLYLIHLVRDIRGFVWSQRKSFQRDERAGVAHDLSPRPVWKSALV
                     WLFVNSISERVIARHDLERTLFLRYEDFANDTLQTSLKLASFLNIAAQPWLEMMTSHK
                     PLQAGHVVAGNRVRMQRHINIKPDNDWQTHLTTFQKVLCWFLAGWKARQYGYTWEPVA
                     ARTTTTGGHTLAGPHYVHSPRTTLDTVATPSENQYGLHKP"
     gene            20144..21553
                     /locus_tag="PLD05_07335"
     CDS             20144..21553
                     /locus_tag="PLD05_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOP77287.1"
                     /translation="MFFKPTHREKAECRQDEEAALGLRSTTLPPLRLSLNFCWTFFGN
                     ITQALSRLLLLVVIVKWEGLETVGLWVLTTSLCSSVFTCCELGLRPLLICDVRRVHPF
                     QDYYSLRLVLALAGAVAVVAFGGFSYGPGTALGLLAIVAVGRTFDSLSDICYGLLQRE
                     ERMDRIGAGMALRYAGGMVGMLAALMLGYGLVIAALVDSLVAALVYWFWSRRNAKTLL
                     AGFRRPANPAVAFIVEDLRWSPPRRVWLSLLWLSIPLGIVAIEINLATNLPRYLVDSI
                     LGRESLAIFATLLQFAAAGMIFVQALGNALAPRLTRYYQSNDARRFWGLLARFLGMTT
                     LLGMLPLVLLWTESGRNILAWIFCPQLLEDPAAARWLALSVCLLYVTGPLGRGVTSVL
                     RFHSHVVIRGVTLGLMLALIPPLATTYGLSGAGMGLTLALLLTLPLYGWVIVKAWKIG
                     GHSIALPQRDQRTTHRAAA"
     gene            complement(21582..>23471)
                     /locus_tag="PLD05_07340"
     CDS             complement(21582..>23471)
                     /locus_tag="PLD05_07340"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOP77288.1"
                     /translation="PEDGEFCKQWSSWDRASRYDEASGKYVSWDANGDGHHFIREENG
                     MQVLAEMEGPGCIWRIWSALAQDGRVKIYLDGSEQPVVDLPFKKYFTGDTPPFNYPQL
                     SYHLEEQGCRGQNLYMPIPYQKSCKIVAEKGWGRYYHFVYRTYPKGTKLPTFSPQLVA
                     EHAELLGKINQFFANNMGDLPPVYAGQEMERHGGVLTLAPGAKERLELAGPKAIVAIQ
                     GKIKTGDRADEMAALRRVVVQITWDDQNKPAVWCPVGDFFGTAPGWNKYKSFLTGVTD
                     EGGYTYWYMPFAKRAQLEVINEDNVPREVEYKITVAPLNRPFEGLGYFHCKWHRDTFE
                     VPPDRWPDWSLLRTEGRGRYMGVMLHVWNPLGGWWGEGDEKFFVDGEKFPSTFGTGSE
                     DYFGYAWCDPHLFQRAYHCQTMTQNNKGHQSVFRWHLADNVAFHTSFDGYIEKYYRTE
                     ERGTQYAVTVCWYLDPNGHDPYEPVPVENRHDYYTKRALKIAGFKILNEAPGNLEAQG
                     MRNFPLGKWSNDNHLWWTGAKPGDVLEVGFDAPKAGKYQVAVILTKARDYGIVQLFVN
                     GQKAGDPIDLYNPEVIRTEPIPLGTFELKEGENVLRVEIVGANEKAIKSYMFGLDDVV
                     LTPAP"
BASE COUNT         4945 a         6560 c         6557 g         5309 t
ORIGIN      
        1 cttagctggg tcatatccga cagaaatcgg gcgctgttgt cccgacgatg actgaatcat
       61 tttgaagcac tggcatctgc cccgaagcga tcccccacct gtaagtgata accgcgaatg
      121 aactcgtcgg ccgacatcat gcgacggccc gcaggttgaa gctgggtaat gaccacaccg
      181 ccttcgccgg cagccacgac gatgcccgat tttccggcgc taaccacagt ccccggcacc
      241 acgccgggtg gtacatcatg ggtgaccgac acgggaccca agatcagttg taggggaggc
      301 tttccgggcc gatgccagta tgtgtacgtt ttcggccagg gttcaaaggc ccggacttga
      361 ttccggatgg cctcggcagg tcgggtccaa tccaccaagc catcgctttt tttcagacgc
      421 ggggccttcg aagccaactt tggatcctgg ggcagggcct cgagaagacc ggcttcgagg
      481 ttgtccaagg cgcgacggac aaaccaggcg ccaatcttgg cgagcttggc ttcgacttcg
      541 ggtgcggttt cgtctggtcc aatttcgacc tcaccctggg caatgatcgg cccggcatcg
      601 acttgtggag tcatgtgaat gacactgacg ccggtggtct tttctccgtg gtaaatcgcc
      661 caattgatgg gagcagcgcc gcggtatttt ggtaacaacg aagcgtgcaa attgatcccg
      721 ccaagtcggg cagtcgctag tgtctccggg gccaatattt gcccataatc acagacgata
      781 aggaggtcag cgtcgaggcc ctccaggtat tggcgggctt cgggggcgtt aatgtcctcc
      841 gggtcataaa tgggcgtacc atgggccgcg gcaattgccc ggaggggact cggcgggacg
      901 ggccggccat gacgtacgag gggcgcagtg accaaacaca ccacagaatg gtgggtattg
      961 taaagctctt cgaatgtggg aaccccgaag ggacccgttc cgaccatcac cagccgcatg
     1021 ccggcgcccc gcgcgacgaa tcaacgagtc ttaataggcg tcgtaccaac acgagtcagc
     1081 acttaaggat cacacaaaaa acaccattgt cgtggggaag accaccattt gtccactggc
     1141 actccccatt gtgggctgat gccttgccta tttcgcctga gttgatcgat ccggcttcca
     1201 aattaaaaaa aggcagccac gggggcgtcc atcccgaagt ggaacacaca tacccgatga
     1261 tgaaatatca cccctatctt acgatgacgc tcatacttgt ggggctcgcg acctgatggg
     1321 ttcaaacgtc gtgccccggg attcaatttt tacagataat agttgcatcg cgggggcgtt
     1381 tcggggaaaa tgttttagct ccgccaaact cgtctggcca cgacacggaa tgtcttcaac
     1441 ggcgtggacg cgtttgagca gcttgagaat tcggggagtt cgttgatttc acacgagggt
     1501 tccgcggagg ggaatgactt ctggtccgta tcgagtgttc ggaaaggagg agcacgttat
     1561 ggtaacgcaa gcgaaaaggc tgcttttggt cttggtggcg gcagcgacgg tcctaatggc
     1621 caccggttgt cccaagcctg cgacagaaca aaagccaacc cgccccagca cgacggcggg
     1681 cttggaaatg gaaacaccat cggagaggcc gcaagcaccg gtaaaagagg aatccgcgga
     1741 gcccacggcg ccctcgcaaa cggagcctgc cgcagagccg ggccaaatgg cgccaacgac
     1801 ggaacccgct gctgaagaga aagccgctga gaaaacagaa gaagacacaa aggaaaaagc
     1861 aaccgagggc gcggccgccc cggagatgcc ccaaaccact cccgcagcag aatcacaagc
     1921 gcaagcagtt ccgcaaggaa caccctttga ggtcaaagta cctttgggtt tgccgcccct
     1981 gcccatccca gaggataacc cgatgaccgt agaaaagatc gagttgggaa aaatgctcta
     2041 tttcgacaag cgagtaagtg ccgatgggac ggtctcttgt gcgacgtgcc acgatcccaa
     2101 gatggcttgg gcagagcatt tgcccacaag caaagggatt catgagcaag tgggtgaacg
     2161 aaatgccccc acggtgatta acgcagccta tgccccgtcg caattctggg atggccgcgc
     2221 taaaacgctg gaagaacagg ccgttggacc ggtggccaat cccatcgaaa tgggtcacac
     2281 cctcgaggga atggtccaga gcttggataa cattccgcaa tatcaagagc ttttcaagaa
     2341 ggtctttgga acgggtgtca ctgccgagaa cttcgccaag gccgtcgccg cctttgaacg
     2401 gacgattctg agtggcaatt caccttatga tcgttacaaa gcaggcgacc agaatgcgtt
     2461 gaccgaggca caaaagcgtg ggttgaagat tttcgaggaa tcgggctgcg ctgattgtca
     2521 cgccccaccg ctgtttagca cctatgagtt ttacaacgcc ggaattggaa tgaacaagga
     2581 aaagcctgac gagggccgaa agagcgtcac tggcaaggag gaggatatgg gagcttttcg
     2641 cgtgcccagc cttcgtgagg tcgccaacac ggcgccgtat ttccacgacg gaagcgtagc
     2701 aacgttggag gaggctgtgg ccatcatggc gaaaggcggt atcgataatc cacaccgttc
     2761 ggccgagttc gatactgttc gtgaagccaa tatcacggca gaacagcaaa aggatcttgt
     2821 ggagttctta aaggcactct cgggcgagtt cccggtgatc gagcccccac aattgccatg
     2881 aaaagagggg aattgtgagg actaaagcac acggacggtt tggcctgcga cttccacgac
     2941 gaggcgtccg gtggcacatt cgaacgtgat cttgcgtccc gatgtgccgc cgacgtcttc
     3001 ggcggtaatg gcaatcccgt gctgccgcag gatggttcgc gtggccgcga tgttgtcatc
     3061 accaatttga aggggtccgc ccccgcgaaa catgcaggca ccgccggcaa ttttggcgac
     3121 caagccagtc cggggacacc ctatgtcggc aagctggcgg atgagatagg gtactgcggt
     3181 gtcggcgaat tttcctggtt gaccattttg gccgtgagag tcgggaagga ccacgtgggc
     3241 tattgcgccc atgcccaggt ggggatgcca caagacaatc cctatgcacg agcccaacac
     3301 ggaggtgaac acggcttctt tatcgcctac cgccgtttgg cccattccca cattgattat
     3361 tttgcttccg agcgatgaac tcgatgctgt aacagtcata cgttgttacc tatcttaatt
     3421 ggtgcgtacc aaggggaatg tgatccacag tttgtaagac cacccttcgc aaatcaggcc
     3481 tttgacgcag attacctccg agcgcgatcg tgtgacactg atgcgacact gcgttccaat
     3541 ttctcacgca ggctcttgga tggaataaac agcacgtgcc agttgaggtt ttcaccttcg
     3601 cggtgaaacc cggtgcggca aagaagggcc gtgtcggacg acatggcctg ttccacaagt
     3661 gcctgctcaa gaacacttgc gaaataatcc cgaacgaaat acggcggtga agggatcaga
     3721 atctgaccgg ttaaccgtgt gagggcgttg acataggcgc aacccaaaat attccccgtc
     3781 tccgtcaggg ccgagatttc cagttcgtcc cactcaggag tggtgtttcg ttgccgacgt
     3841 gtgagggtgg ccgccaattg acgggcattc tcttcatcga atgtgaggat taggaccgtt
     3901 ccaggttcag attcaatccc caggacgacc atggtcatga agtcatcgga aaaccccagt
     3961 tctgctgtaa cctcctccag tggcaattcg cagatgtcat cgagcgttaa tgaaataacg
     4021 ccgtcggtcc atcgacacat agcagccgat gcctcatgcg tggcagcagc aaacagaggt
     4081 cgcaagacct caacgcggtg gattgtcgtt ggattcatag acgcacttcc ttcacgatca
     4141 tggttactgg tgaatcaatc acgttggtca caattggcaa cgttttgtga gttacttcct
     4201 aaaaagtcat catttggctt atgacgacat cgcgtgactt cccacacggc gtgtgatcat
     4261 ccccatgatg gcagcaacat ccagaataag agaaacgcgc ccgtcgccga ggatactcgc
     4321 cccggcaatt cccggtatgt tgcgataatt ttccgcgatg gacttgatca ccacgtcctc
     4381 ttctcccagt acccggtcaa ccaccatgcc aatttcgtga tcggcatcac cgagaatgac
     4441 gagcgtcatt tcgcggtgct cctcctggtc aatttgcgac cggctcaaag tcccaccaaa
     4501 gatttccgag agctccacaa cagagatgac ccgttcgcgg atgcgggcgg tcctcagtcc
     4561 atgcacggtg aggagctgct cagacggcac ggccacaatc tcgttgacgg cctcgatggg
     4621 catggcgaag acatcgccat cgacctggac cagcaagctt ggcaagatag caagcgtcaa
     4681 cggcaacttg atggtaacag tcgtacccga gccaggcaca ctgtcgaggt cgatcgtgcc
     4741 gctcaattct tcgatcttgg ccttaacaat atccatgccc atgccgcggc cggagacttc
     4801 ggttactttc tccgccgtac tcaggccggg aaggaaaatc agctggaaga tttcctggcg
     4861 cgacatcctc tcggcctgac cgggcgtgat caagttccgc tccagggcct tcttgaggat
     4921 tcgctcggta tccagtccct ttccgtcatc actcacctga atgatgatcg aattcccgcg
     4981 gtggaaggcg ttgagtgtga ttgtgccctg ctcgggcttg cccaattgac ggcggacctc
     5041 gggcgactcg atgccgtgat cggccgcatt gcggacaagg tgaatcaatg ggtcgcccaa
     5101 ctcgtcgatc atccgcttgt cgagttccgt cttttcgcca ttgatcacca actgaatatt
     5161 cttgccgttg agtcgggtta catcccgaac gacacgcttg aaacgattga acaggggacc
     5221 gatggggacc atccgggttt ccatgaccgc ccgctgaatc ccatccgtga cgcgatccaa
     5281 ttgatgaacg gcttcagcga gatagttaac ggtacttctc gcctggtcaa gggcctcgag
     5341 ttcgtgggcc aaggcttcga attccttttc aaggcgatca gcaagcgtac tctcgaggcg
     5401 gctcgtccca cccgaggaag accccagaga tgacatgccc gcgattctcc caacaaggtc
     5461 gcggagaccc cgcagcacct gcgacgccct gttgctttgg aagaatccct tgagcttgtc
     5521 gttcaactcc gtcagtcgtg cccttgtaat caccaactgg cccgcaagat tcatcagttg
     5581 atcgagacgc tcgatatcga cacgcagcgt ttcgacggcc ttgacggcct cacctgacgg
     5641 acggctcttc tcttgatcac cgccgccatc gctaaccgtc ggcttttcct tgccttcggc
     5701 caccggctgt tttggaacca tttcggacgg tttggccgaa ctccccgctt tgtagttaac
     5761 cgcgccgcta tccttcgata acggctggat atgaatctgt ttgacgccgc cgactcgaat
     5821 agcgcgggtg attgattcct caggctggct ggtttctatg ataaattcca cgtagtcgag
     5881 tttctcaatc tggtcgagct gttccgaggg taccgaaaac cacacgacgt ttccttgatg
     5941 gcagagcttt tcgtaaatca gccgggcttt gagtccactt agatccagat tggtctgaaa
     6001 ctcgacacgt ccgacgaggc taggaccgct ggtcaaattg cgcgccgttt ggagcaattc
     6061 ggcgctcggc cagacgccaa gtggctgatt tgtttcgcga ggtgatggcg tcggcactgt
     6121 aagcgacacc gccgcggcag ggggattctt cagcgagcta gcgcaagcca ggagatcggc
     6181 ggcaagacta acaaaatccg cgtcgtattg ggactggccc tgtcgcagtt gctccaagaa
     6241 tgagcggaag ccgtcggtgc atttgagaag gatgtcggct aattcgcggg tcaaggggcg
     6301 tcccgtttcg cgcagtccct gaagaatatc ttccatgaga tgggcaagtt tggacgcacg
     6361 atgaaagcct acggatgccg ccgacccctt aattcggtga gccgtacaca agagattctc
     6421 gagctgtgtt gacgtcccct ggccttccgc ctccagaaga gtctcagtaa ggacgtccag
     6481 accttgagcc aattcgtcaa taaataaggc gatataccca gaggaaaggt cagcttcgtc
     6541 gtggacgtct tgaaatcgtg cggcggcctc ttcctgaaaa cgggcgttat cagtgggcaa
     6601 cgggcttgcc tggctcaccg cagaagaatc tggggccgag tcgctcatgg cctcggccaa
     6661 agcggtttcc gcctgagaaa gcgcctccat agcccgggtt tcagcaacgg aagggcccgg
     6721 ctggcgacct tccagcatgg ctgcaatggc cgtgacaacg tcctgggcgt caatcgcatc
     6781 ggagttctga ttgcgaagca gatccaccat ggcccgcaag cggtcggtgg cctggaacat
     6841 caactccacg gcctcgtcat caacgaccat ttgtcccttg cgaaccgcgt caaagaggtt
     6901 ttccaacttg tgggtcaact gattgatatt ggaaagattg agcatggcgg acagtccctt
     6961 aagactgtgc gccgcgcgga acatttcgtt cagaatttca gcgtcaaact gttcgcggct
     7021 ctcaggggcg tggttcttga cccactcgtc caaggtcaga agattctcgc cgaggcgatc
     7081 caaaagctga gtcgactcgt cgaggaaatc cgccagcatt tcctgataca tgtcgtcact
     7141 gacgtaggcg tccataggct ggtctcacct aatccattgg ggaaagttac tccgttggga
     7201 agcgtcttca cagctggtga gaatcactcg gtgggaattt ggataaaggc gtggggctcc
     7261 gcttatttcc gtcgccactc aacaatagct catatttggg gggatgtcag aattacctgc
     7321 ggaaagcaca aggatgcgaa gactgtcccg atggagacaa ggaacggatt tctgggtaat
     7381 tccgagcgct ttttcgaaaa acaaggaaat tgggagactt gggcttttcc taagcgggct
     7441 tcgctcggta aacttagcaa aagatcgtgg ttgcgattgc gccacgctca cattcaatat
     7501 ttttgttgta tatttctttg ttgccataga agaatttgac atgagtgact ctgagcgtac
     7561 tcaggaagtt cgggggccca tcgagccaac agaaactggc cagggatttc gtattccggt
     7621 tgccgatcgg gttaatcggt tgccgcccta tttgtttgcg cgaatcaatc gcctgctgta
     7681 tcaaaaacga cgcgctgggg ccgatgtgat tgaccttggg atgggaaacc cgagtgattc
     7741 gccggaggat attgttatcg aaaagttggc cagtgccgcc cgtgacccgg acaatcacgg
     7801 ttacacggag tcccgcggga ttcttaatct tcggcgggag gtggcgtcaa agtacttcaa
     7861 gcaatatggg gtccggttag acccggaatc agagatcatc gtctgtctcg gttccaaaga
     7921 cggatttagc cacttgtgct tggcattatt aggcccaggc gacacggcaa tcgtccccgc
     7981 cccttcttac ccgccgcatg tgtacgccgt gattctggcc ggtggcaacg tcattagtct
     8041 cgatgtcacc gacccggagc gatatctttc gcacctggag tttacctgcc gcaatctatt
     8101 ccccagacca aaattggttg tggtcaattt tcctcacaac ccaaccacca ccgtcgtcga
     8161 accgcagttt taccacgact tggtcaaact cgccaagcgt tttggcttta tgatcgtcag
     8221 tgacctggcc tatgcggatg tgacctttga tggttatcgc gcaccgagcc tgctcgctgt
     8281 tccaggggcc tttgacgttg ccgtcgaatt gacgacaatg agcaagggct acaacatggc
     8341 tgggtggcgg gtcggttttt gtgccggtaa ttcggagatg atccgtgccc tagccaccgt
     8401 caagacctac tacgactacg ggatgtttcg ccccattcag attgctgcga ttgtggctct
     8461 tcgccatacg gatgcggctg ttgaggcgca agcgaaagaa tatcagcggc gccgcgatgt
     8521 cctgtgcgaa ggcctggagc gtctcggctg gccgatccag aggcctaagg ccaccatgtt
     8581 cgtttgggcc aagatcccgg agccctgggc cagtcagatg agttccttcg atttcgccat
     8641 gaagcttctc aaggaggccg acgtcgccgt tagccccggt ggcggattcg gacccgccgg
     8701 agaaggctac ttacggctgg cccttgtcga gaatgaacag cgattaaagc aagcggttcg
     8761 ccaaattggc cggtgcctcg cccgggcagg tgctacttcc acgtagtgcc cgtacctagc
     8821 gcgcgaagca attcgacgcc ggcattaagc cccgtatccg accgcgcttt gatctcatat
     8881 tgcgcaaaaa aagaaccgaa atcggttctc cgcgaaaatc caaaatgcga aggccgatcc
     8941 gctttccccc gctacgcctt cttttgctcg agtcgcgcca gaagaattaa gccttggcgt
     9001 tttgcattag ccgggcgcga atctcttcca gctcttttga aagccggtag tgcaaaggac
     9061 tttcgggccg aagatgacgg agcagcgact ccgcccgctc aatcgtcttt tcgttgatag
     9121 gccctttggc gaacaacgag taccaactct tggcgacctg ttgttctgtg agcaagtgtg
     9181 acctcccgaa aaataactac ctgagacggt cccttataac ggcggtatta ccgtcggaga
     9241 cggcgccttt tataacattg tacgatcgtc atctcccttg tccatatcct gtctattact
     9301 atcggtacgt ttggtccttc ggcttggttt ggaaaaagcc tcccattttc cgcccccttc
     9361 gcctggccga atgggggagt tagagtctat cccaaaaagt cccctctccc nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccctctccc gccgaacggg agagagaaga
     9541 ttatgggata ggctcttagt cgcggggtgg gcatgccctc aggttaccgg gggtcgctca
     9601 cagcgctcaa tttcgtaggt tacgaccgct tggtctgatt cccaagcgtg cttttttgat
     9661 gttgacctat cagcggcttt ccgctattta gcgaatccca ttgggacggc gtgacaccgg
     9721 cgcgcaccgg tgaacgcgtc gccttccttt ctctggagcg agctccaata ctagtttgga
     9781 tcaaatttaa cggaatgcct tatggcagat tggcatagat cacagtttgt aggcgctcaa
     9841 ccgcactgtg gcccaaatcg tctgaccggg cttatcacca aacagcaacg gctgacctct
     9901 gggacacttt ggtccgccgt gctcagcctg acagtgtgtc ttctctcatt tgtcggttgt
     9961 gccgagcgga acgtttaccg tgccacacgc ctacccttgg aatatcaagc ccccatcgtg
    10021 caggatttgc gcgaaatccc cttgactcag ttggtcgctc agcagagccc aagtgacgtg
    10081 atcgcatccg gggatgtcct cgaaattact atcaacacaa gcctgaacac aaaggcctca
    10141 ccggttacct gtcgggtcga tgatcagggt atgatctttg tcccggatat tggtcccgtt
    10201 tccgtgggtg gacgacgcct ctcagatgcc gaaagactca tcgccgaaac cgcaattgcc
    10261 agggatgtct atcggcagcc tatcgtcaat gtcgtgcgaa aggagggccg gacgtatcgc
    10321 gtaaccgtca cgggagcagt ggagaaaccc ggggtctatc acttacccgc ctcccaagcc
    10381 gacttactga cggcccttgt ccaggcgggc ggattgacaa agcaggccga gcctaccgtg
    10441 gagatccgcc gtggacagaa cattaccgag gtgagtgtcc ccttggaaaa tcccgccgaa
    10501 aacgcggttg tggaaggccg actgacatcc ttcgaggaat caacgactaa tacagtccgc
    10561 atcgatctca tctcggtgag cagttcaccg gcgtcggatc gtcgctatca gttgtttgat
    10621 ggggacgtga tttatgtgcc ggagcggaaa gtggacccca tttttgttga tggtcttgtg
    10681 acaaagcccg gtatgtacga atggcccaat aatcaggaag ttcgcttact agacgctctg
    10741 gccatggccg ggggaaggaa caatcctctg gccgacaatg tgtggattct tcgccatgtg
    10801 ccgggtcgcg aggaacctat tgtgatcaag gccaagctca atgccatcaa aagaaacgcc
    10861 gcggaaaaca tccggatagc cccgggcgac gtcattacgg ttgaggaaac tccgggaacg
    10921 ttcctttaca gtctgctgaa gggattcgtg catttgggcg gttccatccc gctgaattga
    10981 tatgatgggc ccagaggcca agtcctaacc aacgttcgac aaatcagtca tgacaagacg
    11041 atctatggca tgaacgattt tagcctcgcg tcttctgcag tccccgagtc aacgacccat
    11101 ctccttcagg tcgccgtcca atttatcagt gcggtacggc ggcgttggca cattgtagcc
    11161 acctcggtct tggcgacggg cattctcgga gcactctatt acgccacagc cacgcgatat
    11221 tacacttcca aggccgaggt gttgatcctt caagctggcg acaaccccct ttcgccgacg
    11281 attcagtctc agaacaaccg tgaaaacaac ctgatgccca ccttcgtcaa cctcatgacg
    11341 agccagaagg tgctcgaagg ggccatccgc caactttccc cggaagattg ccttgtcgat
    11401 ctcaacccgg ataagccgga acggtggcct gcccaactcc gtgccggatt gacggtaacc
    11461 tccgtgggac gaagcaacat catcgcggtt gagtacaaat cgcgtcatcc ggaggtcgcg
    11521 gcgcgggtcg tcaacgccgt tgttcagtcg tatttggact ttatgaggac caccactcag
    11581 ggcaccgcag cggaaattgt gcatgtcctc aatacggaaa aggatcagat cgccgcccaa
    11641 ttgagcgaaa aagagcaggc cttacagcaa ttgagtcaac agataggggc cttagacacg
    11701 ggggtcaact cgcaagcaat tcatcctttg gtagaaaggg cgagggcctt caacaatgca
    11761 ctggtcgagg cacagaagac tcgagtaaac ctcgaggcca ccatccaggc ggttgaagcc
    11821 gccctccgca atgggcagga catccgcgag tatttgttca gtggttcgga cacggtcggc
    11881 cagcaactct tgcttggcct tctcggtttc gaccgagagg aaagtattgc ccgggcaaac
    11941 atggaacgtc aactgctcga agaccgggtg cagttagcca acgtccaaaa atatttgggg
    12001 aataatcacc cccaagtgca actactgcaa cagcgcattg ccgcaaccga ggattatctt
    12061 cgcaactatc agcggcgaat cgaagcacgc ttggcaatgc tcggagactc cgagttgaca
    12121 agccttctat tagggacact ccgccaccgc ctccaagaga gtgtgcaaaa agaacacgct
    12181 ctgcaacttc agtacgaggc cgcccgccag caagccgcaa ctttagccgg cggacttgcc
    12241 cagatggaaa tgctcaagaa cgacattgcg ggcctgcgag agctttacag tgggctagtc
    12301 cggcgtatca ccaatatgga tttgacaggc gatctgcgag cagccctcgt gacggagccg
    12361 gtcgccaatc cgacgcccgt ctcacccaat ctctttcgcg tgatttctat ggtccttttg
    12421 ggtgggttga tgttggggtt catggcggtc tatgtcatgg atgtccttga cgaccgcttc
    12481 cgaagcatcg aggagatgca gtggcaactt cgtgctcccg ttctctcgat tgtacgtgaa
    12541 atgcctcctc gaagtggtca gggccttgag gctgtggaag tggcggctga cccggatagc
    12601 attgccagtg agagctttcg cacgctgcga acggccctcg ccctcagcga cgcccccacg
    12661 aactcggttg ttatcaccag cgttcagccg ggtgacggta aatccaccgt ggccgccaat
    12721 ctggccgtgg cttttgccca agccggtaag cgaacactgc tcattgacgg cgacatgcgt
    12781 cgcgccggct tgagtacgtt ccttggcttc aagggtcagc agggcttgag cgagatcctg
    12841 cgggaaacgg cggctatcga ggagagtttt gtctctcggg tacgggcgag cttggcagca
    12901 aacctggacc tgctgccttg cggaagtcgt ccggccaatc ctgccgagct tctctctagc
    12961 caacggctca ccgaactctt gcaatgggcc acaagccgct acgaacaggt catcattgat
    13021 acacctcccg cttcactggc cagcgatact gccatttggg ggcggtcggc ccacggtgtg
    13081 gtctttgtcc ttcagcccga caaagataaa cgccgtgctg tccttcgtgc agtcgagtac
    13141 ctggccatcc tcaagatccc cttgttgggt ttagtggtca atcgagtcaa gagtcaaagc
    13201 gaaaaagaat atgccgggta tgccggcgag tactacggcg aggaatcgaa tccgagcgat
    13261 gagaccgccg acactccaga ggcccaaacc acggatgccg tgccatccaa cgagcatcat
    13321 cataggcgaa tcgttccacg aaaggtggcc taggtcgcat tctttcccag ggagacaata
    13381 agcctgacac gaatggcgag agaccctctt caacgaccca aacgagtctg gacccgggtg
    13441 tccgacgcag tatttttggt cgggctttgg ggggcactct tgctgttcgg gggccggcat
    13501 ccctggggtc acgcggtgct tattgtggtc agtcttgccg cggtggcact gacgatgacg
    13561 caacggatga ttctcaaaag ctggaccaca cgccctctcg ggctgggatg gttggctctg
    13621 gcgggtgtca gcattccact gctccagctt attcccttgc ctgaaaactg gctatcgttc
    13681 ctttcgccgc agcttagacg cattctccca gcgtggaacg acccgacaac ggcgagcact
    13741 ctcggcgctt ggcggtgtgt aagcctggca ccggccgaga cccagacggc actgtcactc
    13801 tggctagcct attgcctcgt cttcttctac gtcatccaac gcatcgagga cattgaggat
    13861 gtggaaagcg tccttcgctg gttcgtgccg attgtccttg tgctttccat cttcggattg
    13921 ttgcagtatc tgtttagcaa cgataagttt ttctggttgt acgaacatcc tttcgccaag
    13981 actaccgatg ccctcaaagc gagcttcacc aatcgtaatc atttcgccga tttcgtggcc
    14041 cttaccgctg ggctgctctt gtggtggtcc ttggccgatc gacggcccca cggtggtagc
    14101 cgtcggggac ccactgcctt gtgtgaacat caatcgttgg agggcgacga tacccacaaa
    14161 tacctgagat gcttctcctt gccgctcgtt ttcttggcct tgctgttgac acagtcgcgc
    14221 gggggaactt tggcggcggt aattgccctc gtgatcgcag gcgttacttt gtttcgtaca
    14281 ggttggttga aggcccagtg gctcggggcg tttctgctag ggctcgtttt ccttatgacg
    14341 gcggtcctga tcgtgggaac cgatacaatc gctggacgtt gggaaggcct cgtgaatctt
    14401 tcctgggaac aactcgaccg cggtgaagcc cgacgaacgg tttggcagaa tacctggcgg
    14461 gcaagtcagg atttctggcc gcttggcagc ggttgtggga gcttttcggt tgtctatccc
    14521 ttgtatcaac cgagccgttc gacctcactc tattacaccc atgctgaaaa tggctacttg
    14581 caggtactat tggaagcggg gcttcccggc gtgttcctta tcgccctaac atgtgcgacc
    14641 ttagtaatat ggggctggcg ggcatggcga gctgcaccaa cgcctcgtca ccgggcggcg
    14701 gtaggggccg tgggagcagg cttggcggcc ttcgcattcc acggactcgt cgattacgtt
    14761 tggtacgtcc ccggcttagc cgcggtggtg gcgatcctca tagcctgtct ggtccggctt
    14821 taccagatga cccaactggc ggcgcaacac cgcactgctt ccgcgaccat gtccctcgcc
    14881 tctccctcgt ccagtctcac ccttcgctgg accagcttca cagtcgcctt cattctttgt
    14941 gctgcgtggc aaatcccggc tgcacagcga agcttcttcg ccggcatcgc tgaattttca
    15001 tggcatcgct tccttgctct ataccgcacg gccgattggt ctgatctacg aggagccatt
    15061 caatcaacgg tagcaagcaa tgttgtgtct cccggcgacg aagaactaat ggattccgaa
    15121 aaccccggtt tgtccgcctc cgatgcccca gccgcctcca cagccacaac gatgggattc
    15181 caaggggagg gcccaacaca actggccaca gagcgggaga tgatcgacct tctcctcaaa
    15241 gccatccatc gagatcctcg ccgggcggag gcccatttac gattggccaa ggcccatctc
    15301 cggatgtttc acttaatcca ggaacagaac gaaaacccca tgccactctc ccaaatccgc
    15361 gatgctgtgt tcctttccca gttttcctcg caagaagaat tggatcgctg gttgcagaca
    15421 gcgatcggaa accacgtcac acttttgcac tcagcaaagc aagaagcgct ttcggctgtc
    15481 cgtttgtgcc cccttttggg agaggggtat tttattctgg ggcaactcag ttttttaaac
    15541 ggcggtcgag aggaagatgt aatggcctgg tttcgtcagt ccctggcggt gcgacctaat
    15601 gatggggaat tgctctttcg ggtgggcgcc gagcttgctc tcgccgggca gtaccaggag
    15661 gccttagagt tgtggcgccg ggcttttcgc tgtgggcggc tgtatcagat tcgtttactc
    15721 gagcgactca tgggttccgt tccgccgggg gaaatcgatc aggagatcac attcctcatc
    15781 gagacgttcg agccagacct tgaaatgctt cgctacatgt atcggcaata ccgcaaacgg
    15841 tatcccgtgg cttccctcga tccgcttcgt cgggcgtatg ccgtggcgct tacaagtcaa
    15901 ctgcgtgacg agaggaaccc cccttcccag cgggccgagc tatggttgga attgcatttc
    15961 ctccatcttg acgccggcga gatccaggcg gccctacagt gtgccgaaga ggcagtgcgt
    16021 tgcaacccac tcgactatcg ggcccaccat tatttatcac ttcggctaga agaaatgggg
    16081 cggttcgccg aggccgagga acacgtccgc tggtgcctgc aaaggcggcc agggcatcgt
    16141 tcgcttcaga accggcttca caatctttat caaaaaagaa tgaaggcgga ggatgaagcc
    16201 cgaacttggc ggatggcaga ggccgtatct ccgggcggtt atccacccaa ccgatgaaga
    16261 cagccgttcg tgtcctcgct gttcgacgga acatcccgtg caatgttacc ctttttggga
    16321 cgtctgcacg cgatatctct cttaatttca aatcctgtgt ttgcgtaaaa gcctacccat
    16381 aatctcccct cttccgttca gcgggagagc attcgggggt gagggtggct cgaatcgctg
    16441 ccggttttct caatcgactc cgacgattgc ttgccctcac cccagccctc tcccagaggg
    16501 agagagagac ttcttgggat gcactcttaa tttcaaatcc tgtgtttgcc caaggaagaa
    16561 acggtcttgt cacatccgtt accatcgggt attcattccc tgcggccgag acgcctaaga
    16621 atgaccacct gacatggagg ttggcgtgga gcttaggaga cacccgaaat ccccccaatt
    16681 ttccccatcg agccagccgg accgacctac tgtcgtcggt tcactgccgg cgttacgtct
    16741 tgtccacgat gaggcgagca gtcactgtga ctttgatcct gtagccggtg ggccaacgag
    16801 tgggaatggg aaaccaattg cggatcaaaa gcaactcggt tcaaattcac aagacgctgc
    16861 ggttagcctc cgatccgtga tggggaatta cctcgcacgg gcactcgatc ggctctcgtc
    16921 gatgtcgtgg ataaggcggc cttttcgcga acaatggcgt ggggccatac gcgacctcat
    16981 ggggctagcc tggttatcct tggccttttt ggtcagttat ttcgtcagtt actacgttcg
    17041 atttgatggg gactcgtggc tgtggatcaa caagtggttc ttgaacacgg tcgcgtatgt
    17101 ctgcgcggtt aagttgctgg ttttttgggg gtttcggctc catcgtagct ggggccgatt
    17161 tctcacattt catgatctcg tgttgcttgt cgaggccacg acggtcgccc tcgcggctgt
    17221 ggcccttgtt gatcggcttc tcttgccccc tcggatgatt ccccggggca tcctccttat
    17281 tgattggggg acaacgttgt tagtgttagg gggatgccgg gcagcgattc gggcagtttg
    17341 ggacggtggt tggcataatt tctttacgaa tgcttcgccc gtgcttattg cgggcgccga
    17401 tgaatccggc gaggaactgc ttcgggccat cagtcgctac caggcgacgc ggcatcgggt
    17461 ggtggggttc ctggaccgag acagtcgccg gttgggtaac cggattggag ggattcccgt
    17521 tgttgggaca tttgatcaag cttgccaggt ggcgacacgg cttggcgtca aagagatctt
    17581 actcgccggt ggtgctttga ccggcaagga agtccgccga ttgatggaag aagcacgccg
    17641 tgcctccatc gaggtcaagg tcttgccaag ctacgagcag ctattagcgg gtcggattgc
    17701 cgtccagccg cggcctattg cgattgagga cctacttcgc cgggaacccg taacactgga
    17761 atggaaaaat atccaccgtt ggctggaagg tcgaacactt cttgtgacgg gaagtgccgg
    17821 cagcatcggg gccgaaatcg cccggcagtt gttgcattat tcgccaagat gcctcgttct
    17881 attggatcgg agtgaaacgg ggcaattttt cttggaacgg gaattgaaag agatccagca
    17941 ccggttgttg gccaatcaga agagcgttcc cgagctagcg gttgtcttgg ccgacgtgct
    18001 cgatcttcca cgaatgcaaa gcatcttcca tcagtaccgg ccagaggtgg tatttcatgc
    18061 ggcggcatac aagcacgtcc cgcttatgga gtcccatcct ggcgaggcct ttaagaacat
    18121 tgtgctggcg accaagaatg tcgccgacct ggcggctcgg tttggcgcgg agtccttcgt
    18181 cctcatttcc accgataagg ccgtcaatcc caccagcgtc atgggagcgt gcaagcgcct
    18241 ggcggagatg tacgtccaat ccatgacgaa caccacttcc acccgcttcg tgaccgttcg
    18301 ctttgggaat gtcttggatt cggctggaag cgtggtccaa atttttcgtc agcagattgc
    18361 tgaagggggg cccgtcacga tcactgatcc gcgtatggaa cgcttcttca tgaccattcc
    18421 agaggccgcc ggtctcgtca ttcaggccgg ggtgattggc gaaagtggac aaatcttggt
    18481 gctcgatatg ggcgatccgg tgaagattat tgatttggcc atggatatga tccgcctctc
    18541 gggattagag gtgggacgcg atatcgaaat ccgggtgacg ggcctgcgac caggtgaaaa
    18601 gctgttcgag gagcttcggg cggctggtga acggaccgtt cccacgcggc acaagaagat
    18661 tcgtgtggca ctcggttcgc gaatggatag ggcgaccctt gtgcgggcga ttgaacaatt
    18721 gcgagactgg gccgattccg atcctcctcg tgtgatcgcc gagctgaaac ggatcgtccc
    18781 gggttacggc gtctcccaga tgcccgtgcc ggaacacatt cgccgggctg cctaaggtgc
    18841 ctgtttttcg ggaggtgaca tcgtcatccg tcgacacgcc gcttacgcga aacaaaggaa
    18901 acacaaatcc gaccaccggt cagcacccgc aagaaacgtc atagattctc caaaatcttg
    18961 catcaatgat gtgagcacgc cctatgaaaa gccagagaca ttccttggat aaatccatcc
    19021 gatatccgcg tgttgtcttt atcgtgggga cgggacggag cggatcgacc gtgctggacc
    19081 tctttctggg tcagcatccc accattcaga gtgccggcga gctgtgcaac gttgtcaaaa
    19141 gcggatgggt cggcggcgag ttctgctcct gcggcgaacg cgttcccctg tgtcggttct
    19201 ggacggaggt tcgcaagatc tgggaggagc tcacgggagc caaacccgag gactacgaga
    19261 gcctaaggtc cttggtggaa ggcaatagac gaacgttgtg gaagatgtta cgtaacgatc
    19321 ggcccgagat gttgcttctt tcgggcaagg aaccgggcga gttcttagcc tacgcgcagt
    19381 ggaccgccgc cctttatcgt gccattgccg aggtcagcgg aaagaccctg ctgatcgact
    19441 cctccaagaa tccggcacgt gccttggcct tggtgctgat ggatcatcga ttggatctta
    19501 ttgacctcta tctgattcat ctcgtccggg atattcgggg atttgtctgg tcacagcgta
    19561 agagctttca gcgcgatgaa cgcgccggcg ttgctcacga cctttcccca cgacccgtat
    19621 ggaaaagtgc cctcgtttgg ttgtttgtca acagcatctc ggaaagggtc atcgcacggc
    19681 acgatttgga gcgtaccctg ttcttgcggt atgaagattt tgccaacgac acactccaga
    19741 catcattgaa gctcgcttct ttcctcaaca tcgcggcaca gccttggctg gaaatgatga
    19801 ccagccataa gccactccag gccgggcatg tcgtggctgg aaatcgcgtg cggatgcagc
    19861 gacacatcaa cattaaaccg gataacgact ggcagacgca cttaacgacc ttccaaaagg
    19921 tcttgtgttg gtttttggcc ggatggaagg cacgccaata cgggtacacc tgggaacctg
    19981 ttgcggctcg cacgaccacg accggcggcc ataccttagc gggaccacat tacgttcaca
    20041 gtccacgtac cacactcgat actgtggcca ctcccagtga aaaccagtat ggcttgcaca
    20101 agccctgacc tctggtggcc agtaaacgtg tgatcatggc aatatgtttt ttaagcccac
    20161 gcatagagaa aaagcagagt gtcggcaaga tgaggaagcg gcccttggcc tgcgctcaac
    20221 gaccctaccg cctcttcgcc tttcgctcaa tttttgctgg acgttctttg gaaatatcac
    20281 gcaggccctc tcgcggctgc tgcttctggt tgtcatcgtc aagtgggaag gactcgaaac
    20341 cgtcggcctt tgggtcttga ccacatcgct gtgtagttct gtcttcacat gctgcgaact
    20401 tggattacgc ccgctgctga tttgtgacgt ccgccgggtc catccgtttc aggactacta
    20461 ttcgctccgt cttgtgctcg cactggcggg agccgtagcc gtggtggcgt ttggtggctt
    20521 ctcttacggc ccaggaaccg cgctcggact ccttgcgatt gtcgcggtgg gacggacatt
    20581 cgattccctt tctgacattt gttacggcct tcttcagcga gaagaacgaa tggaccgtat
    20641 tggggcaggc atggctctcc gttatgccgg cggtatggtc gggatgctgg ccgccttgat
    20701 gttgggctac ggcttggtga tcgctgccct ggtcgattcg ttagtggcgg ccctcgtcta
    20761 ttggttctgg agtcgtcgta atgcaaaaac acttcttgcg ggctttcgac ggccggcaaa
    20821 cccagcggtt gcgttcatcg ttgaagacct gcgatggagc cctccccggc gtgtgtggct
    20881 gtctctcctt tggttaagta tccctttggg aattgtggcc atcgaaatca acctcgcgac
    20941 aaatttgccg cggtatctcg tcgattcgat tctcggacgc gagtcgctgg ccatttttgc
    21001 cacgttgctg caatttgccg ctgccgggat gatctttgtc caggccctgg gaaatgccct
    21061 ggcaccgcgg ctgacacgat actaccagtc caacgacgca cggcgttttt ggggtttgtt
    21121 ggcgcggttc ctgggcatga caacattgtt ggggatgctt ccgctcgtcc ttctgtggac
    21181 tgaatcgggc cgaaacatct tggcctggat cttttgcccc cagcttctgg aagaccccgc
    21241 tgcggcccgc tggctggcgc tctccgtgtg cttactgtac gtcaccgggc ccctggggcg
    21301 aggtgtgaca tcggtcctgc gatttcactc tcatgtggtg atacgcggcg tcacgctggg
    21361 actgatgctc gcattgatcc caccgttggc caccacatat ggactgtccg gcgcaggaat
    21421 ggggctaacg ttggccctgc tgttgacact gcctctctat ggttgggtga tcgtaaaggc
    21481 atggaaaata ggcgggcact ccatcgccct tccgcaaagg gatcaacgga ccacccaccg
    21541 cgctgccgcg tgaaagaagc gctccgcaaa gccaaatcgc attacggtgc gggagtcaat
    21601 accacatcat ccaggccaaa catgtaactt ttgatggcct tctcgttggc acccacgatc
    21661 tctacccgca ggacgttctc tccttctttc agttcgaacg tacccagggg gataggctcc
    21721 gtgcgaatga cctcggggtt atacagatcg atggggtcgc cggccttttg gccgttaacg
    21781 aaaagctgca caatgccgta atcgcgggcc ttggtcaaga tcacggccac ctgatatttc
    21841 ccggcctttg gggcatcgaa gccaacttcc agcacgtcgc ccggcttggc tccggtccac
    21901 caaagatgat tgtcgttgct ccacttgcca aggggaaaat tccgcatgcc ttgggcctca
    21961 agattccccg gtgcttcatt gagaatcttg aacccagcga tcttgagggc ccgcttggtg
    22021 taatagtcgt gccggttttc gacgggcacg ggttcgtaag gatcatgtcc gttgggatca
    22081 agataccagc aaacggtcac cgcatactgg gttccgcgct cctccgtacg gtaatacttc
    22141 tcgatgtagc cgtcaaagct ggtatggaag gcgacgttat ccgccaaatg ccagcgaaag
    22201 acggactggt ggcccttgtt gttttgggtc atcgtctggc agtgatacgc cctttgaaag
    22261 agatggggat cgcaccaggc gtaaccgaaa taatcctcgg agccggtacc aaatgtggat
    22321 gggaacttct caccatcgac gaagaacttc tcatcacctt cgccccacca accgccgagg
    22381 ggattccaga cgtggagcat cacgcccata taccgccccc gtccttctgt gcgaagcaac
    22441 gaccaatcgg gccaacggtc gggcggcacc tcaaaggtat ctcggtgcca tttgcaatgg
    22501 aaatatccca atccttcaaa ggggcgattc agcggtgcga cggtaatttt gtactcgact
    22561 tcgcgcggga cgttgtcttc gttgatcacc tcaagttgcg ccctcttggc aaatggcata
    22621 taccagtaag tgtagccacc ttcgtccgtg acgccggtca ggaaactctt gtacttgttc
    22681 catcccggag cagtcccaaa gaagtcacca acgggacacc acaccgctgg tttgttctga
    22741 tcatcccagg tgatttggac caccacccgc cgcaaggccg ccatttcatc cgcacgatca
    22801 ccggtcttaa tcttcccttg aatcgccacg atggccttag gaccggcgag ctccagcctt
    22861 tccttggccc caggggccag ggtcaacaca ccgccgtgcc tttccatttc ctgacccgca
    22921 taaaccggcg gcaggtctcc catgttattg gcaaaaaact gattgatctt gccaagcaac
    22981 tcggcatgtt cagccaccag ttgcgggcta aatgtaggaa gcttggtccc cttgggatag
    23041 gtgcgataaa caaagtggta ataacgcccc caaccttttt cagcgacaat tttgcaggac
    23101 ttttgatagg ggataggcat gtagagattc tgcccacggc atccttgttc ttccaaatgg
    23161 taggagagtt gtgggtagtt gaacggtggc gtatcgccgg tgaaatactt tttgaaagga
    23221 aggtcaacca ccggctgctc ggaaccgtcc aggtagattt tcacccgacc gtcctgggcc
    23281 aaagccgacc agattctcca aatgcaaccg ggcccttcca tctcggccag gacctgcatt
    23341 ccattctctt cgcggatgaa gtgatgcccg tcgccgttgg cgtcccagct cacgtatttt
    23401 cccgacgcct catcgtagcg gcttgcccga tcccaacttg accattgttt gcagaactcg
    23461 ccgtcctccg g
//
LOCUS       DAOSUT010000061        22866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1467_length_22866_cov_10.122222, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000061 DAOSUT010000000
VERSION     DAOSUT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_22866_cov_10.122222"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..605)
                     /locus_tag="PLD05_07345"
     CDS             complement(78..605)
                     /locus_tag="PLD05_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOP77289.1"
                     /translation="MGVLAKPDYYFSSVTKIDIPFLRKENIACLLLDVDCTIADYRRG
                     ELDHVVAEWLRQLQEAGIQTCLVSNGGRRRIKRLAGHLGMPFVARSLKPFPWGCRRAI
                     KLLGVSPEQAAMVGDQLFADVLAGRWAGLRTILVQPRSGIKEPWVTRWKRPIERWLLG
                     RWGLLPAQDGTKENE"
     gene            complement(643..3309)
                     /locus_tag="PLD05_07350"
     CDS             complement(643..3309)
                     /locus_tag="PLD05_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77290.1"
                     /translation="MANSSKTLSIPIDLGTWGTPVDLPNVGVPLRFGLPLPRGKLSYP
                     CRGQLVSPSGAVFPTQIAPVASWPDGSARWVRIDTVIHACLTSQWRETWQLDFESLDE
                     PTGQGIPDRIPLRLGYSVVPTSSRDTVFAFVDRASNEPLAVAHVFMKTKAGSVRLGQC
                     RHLRWHVQGPICYEATAEGTFPGVRGLLWRVTLRVYPTLNAIHIKAMLRNTRRARHRG
                     GLWDLGDPGSILLREFGLRLEWRDPLKHLVWRAKPSEVFHIVDGDRWPGKNSTGSRDG
                     DSLIWQVHQASSGGDHWNSFNHVNARGQVTLPFRGYRAISPEGTTSGDRADPLACLQT
                     AAYSCGFWMPDFWQEFPKRIDVGPRSSTLAFLAENPEDVHELQGGEQKTYVAWIVLSD
                     GSDDGRHAVTTQLMALCRRVTVRSALQQALVELPSDLPPPELWPGTALAQFAEEAGFG
                     PKGLLAGREEIDEYGWRHFGDVYASHEKAFYEGRLPFVSHYNNQFDLLCGLILCRLVS
                     NDPRWEELIHPLARHVIDIDIYHTLRDRSTFNGGMFWMTDHYVTAATATHRAFSRENA
                     QRPGYGGGPSNEQNYTSGLTLYYLLWGDEDARDAVLSLANWVISMEDGRQTPFFIADD
                     GPTGLATATSSPDYHGPGRGSANSVNALLDAWLISGNAVYRSWAEKLCRRVIHPKDDP
                     AAFGLLHAETRWSYTMFLEVLCKFLRIKCLFGEHDHTFEYLRRSLICYCDWMVHHERP
                     YLDHPEELEYPTEAWAAQEFRKATVLFLAAKFAEPERAARYRQKAIWFAEKAWEELNR
                     FESRINPRTAAVILNQAIWHLADAVSEGCACCLRLKDESLSLPPREIFIPQRARVRRM
                     LTSRRDWPRIAVRLANPYNWFRLIQILWQWRN"
     gene            complement(3321..3953)
                     /locus_tag="PLD05_07355"
     CDS             complement(3321..3953)
                     /locus_tag="PLD05_07355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOP77291.1"
                     /translation="MSPRLIIKRCVQVLVLSLGVLFWVLYRLEALIWGKRPAFACWSQ
                     CLSLLPGRCGCLVRWSFYFLAARRCSWDVTIEFGTIFSSPNIEIGRHVYIGAYCVLGD
                     VQIGNGVRIASGVSIPSGSRQHWVQVVGQGGDAGGPESPNQYDRVAIGERTWIGERAV
                     ILANVGTRAIVGAGSVVTSPVEDDSVVAGVPARVIRRLSPASSEIGVETS"
     gene            complement(4062..5009)
                     /locus_tag="PLD05_07360"
     CDS             complement(4062..5009)
                     /locus_tag="PLD05_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP77292.1"
                     /translation="MSRPLVSAIIPVFNGAQYVAKAIDSVLAQTYSPIEVIAIDDGST
                     DESPEILAGYGDKICCIRQVNRGVGAARNRGILAARGEYVAFLDQDDWWYPQKIERQL
                     AALANSDAGFVHTHTVYFHDVTQTFCPPLDPEAKPEEYVGWCFEKLLFGNAICNSSVL
                     VRRELFAQVGLCDPIIEGNTVQDYDLWLRLSQVTPFAYVDEPLTVFRLHSHQGTSNHR
                     MMLREQIKVLQRRLPGLPSALRHSVARRLARLYDLLGSYELDYHDHRAARWAFRQSFR
                     WEPHLRSLCLWFVTFLTPRDVAKLRSLYARWKGFRARKS"
     gene            complement(5011..6219)
                     /locus_tag="PLD05_07365"
     CDS             complement(5011..6219)
                     /locus_tag="PLD05_07365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP77293.1"
                     /translation="MALQTPAREVIAHESRQLRIFIAAHSGQQGGAELCLDVFLAHLP
                     RARYKATVLFGCEGPMVQAAAEKGYPTQVVPFVWWFGYEPCSWYWRNLLKSPFRVARL
                     ARLLRQESFDVVYTNSAVIFEAALAARIAHCPHLWHVHEILRPQSWRSWLPIRTICRL
                     IDRWSDLIVFESQAARNIFASLHQPKTKTDAVSNPVRFLTDIEPSHDPAVIRNTLGLP
                     QDALIVVWIGQFIPRKNPQLAIEGFARMRSPRPAILLMVGDGPLRATVESLVPEELRP
                     KLRFLGFQRDVQPIILASDVLLLTSVEESFGLVIVEAAACGRPAIATACGGPEEIVRD
                     GETGYLVPCQDPDALGRALTRLASQPELARQMGQRARTWALERFHPQNFSSRMVAALE
                     SLVATQQQRS"
     gene            complement(6226..7179)
                     /locus_tag="PLD05_07370"
     CDS             complement(6226..7179)
                     /locus_tag="PLD05_07370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP77294.1"
                     /translation="MAETSGPPREFPRISVIIPVFNGERFLGEAIKSALSQTLPPYEI
                     LVIDDGSTDGSAQLAESFGPPVRVIRQENRGEAAARNRGIDAAQGDWIAFLDCDDVWK
                     PEKLALQAALFYSGVDCVHTNFFYFGASQGTVDVGDVPPATRYSLGYVARRNPFRISS
                     LLVRRDLDIRFPEWTQDGEDLIYFLELARKCEIRLVREFVTGYRVHAKGQSSRPDMLV
                     RRFHALQQFLEGVADLPVAEKDLVQREYLALMAEQATLAKHRRNWQEYWALRRFLASW
                     KGPLPAEAKPVLREFIFPPLVYRVKDWFDALTRCDADSGSG"
     gene            complement(7213..8106)
                     /locus_tag="PLD05_07375"
     CDS             complement(7213..8106)
                     /locus_tag="PLD05_07375"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOP77295.1"
                     /translation="MTHSASMDKHLRLFSTDEFQSLWLADGDRRKRPVFLIGIHRRSG
                     TNFLADAICLLPEFALPLPLAEDYVLQYSPLLRQYVVETAEAQYRKRFPNAPAEYERC
                     VASLHRHLGDGLIRFLSQYLPEGKRLLTKTPDPWNLNDFFLFFPEALLIALVRDGRDC
                     VESSKRGLPGHSYNYWITAWAQNAQRILDFFRRLPQSHVGQVIYTQYEQLLSNRGEVE
                     RVIRFLGCDPGTFPWDRFENLPVRGSSFYRGTRQELHWQPVEKGKDFQPVGRWKHWPW
                     WVRWQFRRIAGREFEALGYTW"
     gene            complement(8122..9039)
                     /locus_tag="PLD05_07380"
     CDS             complement(8122..9039)
                     /locus_tag="PLD05_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP77296.1"
                     /translation="MMSHHQNWFIVIRTVGRTDLLGRTLHSLVTAGVSRFPVEIVVVE
                     NGGTRRAAELVKSLAEGCRIQYLAIPEVGQAAASNAFLNRNPGTFAWFLDDDVRVTAE
                     AIEAYHLAAQRGQQGREYYGGPLGIDYETPPPEWLLTYLPRSARGWQPNMEDMATTVP
                     MFLGANWAAWADDLLAVGGFENQFGPGGVGAETALQKKLFQRGMRPVYLPQGLVYHWV
                     PRSRCSPQWALRRAYQIGVAEGLEQPGSEVFLFGYPRWAYRGLMERFSRWCVTRFSRD
                     VHRRFQAAYELWFFLGWMKGKQARRYRPT"
     gene            complement(9313..10524)
                     /locus_tag="PLD05_07385"
     CDS             complement(9313..10524)
                     /locus_tag="PLD05_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP77297.1"
                     /translation="MRILFVAVGGSIHTARWIDQLTDRGWDLHLFPTELYHLRPELKG
                     VTIHDVEDPRRWTGQPGLKFHDRFYAAFGRDWPLPFGRYRASQWITRCRTQFANHAWR
                     LARLIDRLRPDIVHSLGLHLGGYLTLAAREECRKPFPTWVVTNWGSDLFLFGRLTEHK
                     ERLAKVLNTADYYWCECQRDVELAKSWGVPSERILPTVPAPGGFDIEQAEKLRTPGPT
                     SSRRVITVKGYQDWAGRALVAFQALRGCSDLLAGYRITVYTGGRDPEPVRIAGELLSQ
                     DLDIPVTFVPPRTPHEAMLRLHGQSRISIGLSISDSIAISLLEAMLMGSFPIQSNTGA
                     AYEWIEDGKTGLIVPPEDPHAVTEAIRRALWEDHLVDSAAAINQQVIAERCERRKIAQ
                     CVVSTYERICQ"
     gene            complement(10561..11196)
                     /locus_tag="PLD05_07390"
     CDS             complement(10561..11196)
                     /locus_tag="PLD05_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77298.1"
                     /translation="MPSSDPRTLSIVHDIILRLAPRSVIDIGVGHGKTGVLIREYTDI
                     WNERYDKASWQTKIYGIEAFPQYHNALWDYAYDEVRIGDALSVLGTLPDVDLVVVLDV
                     WEHFERDYAQRVLELIMKKARFLLISTPIQVREQGAVFGNTYERHVSTWSPRDFMDVP
                     HRLVACTGFDWVLLLSSGEPIPYEVWRRHRRWEHLRRGWVAFWKILPHKNW"
     gene            complement(11196..12440)
                     /locus_tag="PLD05_07395"
     CDS             complement(11196..12440)
                     /locus_tag="PLD05_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP77299.1"
                     /translation="MSSDVLVEVQHLSKKFRRGESYDSLRDLIPGLVRRLGFSRGQSQ
                     PTDRWFWALRDVSFQVRRGEVLGIIGANGAGKSTLLKILARILRPTEGYVRVNGRLRA
                     LIEVAAGFHPDLTGRENIFLNGAILGMRMAEIKARFDEIVEFAGIGPFLDTPVKRYSS
                     GMFARLGFAVAAHLEPDILLVDEVLAVGDAAFQAKCLGKMQHVAGQGRTVIFVSHNMR
                     AVMQLCDRVILLEHGRITQEGPAEQVVDAYLRQSLAQYQSWVCELPGLPDAPFEVHRL
                     RLVTEDGRPAPVVPRSQPIVIEITGEVRAADRRLIVAIDIKTIDDLTLFRTHSFEQDQ
                     SYAILGRPGPFCLSCRIPAELLPAGKYNVGLVLAERGVREFFHHRQVLQFEVYQDRPI
                     RGSDIMSTVGLVTPSCDWTRID"
     assembly_gap    12581..12680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12681..13198)
                     /locus_tag="PLD05_07400"
     CDS             complement(<12681..13198)
                     /locus_tag="PLD05_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77300.1"
                     /translation="MEQRQADSPQRSPLVSDELGWEWSSSDTRRLGPMFREMVQELWQ
                     YRELLGQMVLRDIRIRYKQAVMGFGWALLMPLLIVGAGCLVKIALARASGDGVDLPRV
                     AGMSVKALGWAFFAGALGFAANSLVSNMTLVTKVYFPRELFPLSAVLTQLFDTLVGSI
                     FLSVLLVVFAHIG"
     gene            complement(13251..14618)
                     /locus_tag="PLD05_07405"
     CDS             complement(13251..14618)
                     /locus_tag="PLD05_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOP77301.1"
                     /translation="MRGLVYVYLMTYLGAVVSLFYPLVGLFIYIHYAILKPESLWYWS
                     VGAQGPGRYSFIVGVGMLVGWAIRGFGDWRLGRSTAVAGCLVGFLAWGSVGYFFCWEP
                     ALAKVFIISLLKIVLPFLLGISLLQTTNHVRWLAWVIVITQGYLALDFNRSYYQGINR
                     IVLEAYGGFDNNCIAAAMVVGTLLAFFLGIAESRWELKTLAFAACGLMAHTIMFSFSR
                     GGLLGLIAGGTAAFLLIPKTFKTTLIYGLAIILALRLAGPEVRQRFMTTFVDPEQRDR
                     STRSRVEQWKACLQTIRDNPFMGVGVNHWPLQSQRLGLPKMHAHSLWLETGAETGIPG
                     LLLLSGFYGLTIFRLWTLIRQCPHLDLWSQNMARGVVAALIGFAVSVSFISVMWLEIP
                     YYTALLGAALLRVHTPTWMGRHEVLAEPTLEAVAAPLDSPADASFPAFRDHSGYGSGS
                     RPRFA"
     gene            complement(14695..15507)
                     /locus_tag="PLD05_07410"
     CDS             complement(14695..15507)
                     /locus_tag="PLD05_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP77302.1"
                     /translation="MADDCCPLCGSAVTSGVDRRRWALWQTLGKRFGIYDIPQDLRLS
                     VVVPAYNEVETIDTILQRILEVPIQKEVLVVDDGSTDGTSERLRRWESQSEVKVLRHS
                     RNRGKGAALRTAFAQATGQIIVIQDADLEYDPAEHLRLIQPIVDGVADVVYGSRFVSE
                     GPHRVLYFWHYQANRFITTLSNMFTDLNLTDVETCHKAFRSEVIKEILPTLKEDRFGI
                     EIELTAKVARRHFRIYETGISYYGRTYQQGKKIGFRDALQAVWCVLRYWWSD"
     gene            complement(15574..16779)
                     /locus_tag="PLD05_07415"
     CDS             complement(15574..16779)
                     /locus_tag="PLD05_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOP77303.1"
                     /translation="MSRKRILYLVHRTPYPPNRGHRIRSFHWLDYLSQRAEVDLAFLA
                     DEPLEEDHLTELRFRCQRVAWVRTSRLGQRMRGILSLLRGRSATEAAFYSPRLERTVR
                     RWLAETTYDAVIAYCSSMAIYLRRLGLSPNRCIVDLVDVDSQKWLDYAETVAFPQAFL
                     YRLEGNRLRRFERELDHLAKFLIFVTEQESQIFREFHPTEKIRVVENGVDLAYFDPAH
                     SGEPTPAPQTPVRLVFVGAMDYYPNVDGVTWFLREIWPRVRNAGDVVFDIVGAGAGKS
                     FWRLVEATPGARLVGNVPDVRPYLRGSVSVVPLRIARGLQNKVLEAMAMGCPVVASPA
                     ALEGLDVAVGEDVLSAQDVDNWVEQLTQLIHSPEMRSRLGAAARAKIEKRYQWPDRLR
                     VLDDLLGLS"
     gene            complement(16945..17838)
                     /locus_tag="PLD05_07420"
     CDS             complement(16945..17838)
                     /locus_tag="PLD05_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOP77304.1"
                     /translation="MPLVHALTFDVEEYFQVSAFERVVSRLRWDEYPSRVGPFVEKIL
                     DILAEHGVSATFFVLGWIAKKYPKLIRLIHSMGHEIGSHSMEHRLVYDLSPGEFREDL
                     RQSLKLLEDHTGERVTVFRAPSFSITSRSLWAIEILAEEGITCDASIYPIVHDRYGIP
                     DARLEPHRIRTDSGIIWEFPGTALSWMGLRLPLGGGGYLRLYPLWLTRRLFQRVIRQQ
                     RPIMMYVHPWELDPDQPRIGGISPVSRFRHYVNLATTEKKLRALLRAFSWGSLRESLA
                     SWQSQGVDPDDVVPMSTLARG"
     gene            complement(17849..18712)
                     /locus_tag="PLD05_07425"
     CDS             complement(17849..18712)
                     /locus_tag="PLD05_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOP77305.1"
                     /translation="MSRLVHGESAAKRSARVEYENGKSRSSGVPRIQPSPYFRWHAIW
                     AWILTALATPVFLPIIGFLVLLVRLTSRGPGLYRQQRVGKNGKIFWIYKIRTMRIDAE
                     AHTGPVWTEENDPRITPVGRWLRRLHLDELPQIFNVLMGQMTLIGPRPERPEFTQMLA
                     QAIPGYLNRIAVLPGITGLAQINLPPDSDLDSVRRKLVLDLEYIEKANFWLDVRILLC
                     TVSKMVGLPGLKIAGWLGLLRPARIPPWMFNGKTGSHQIPCTYSQLQRRSQDKTIHFA
                     EARRNIFRKPR"
     gene            complement(18979..19563)
                     /locus_tag="PLD05_07430"
     CDS             complement(18979..19563)
                     /locus_tag="PLD05_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOP77306.1"
                     /translation="MPIRPREPDLYPDDLLSDPCDRARSQSWWALYTKPRQEKVLIRR
                     LREMEIAHYCPLVAKRVRTPKGRALTSHVPLFPGYVFLCGQEDDRYQAMTTNCVTHCL
                     EVPDVPRLVKDLQNIWRIVQSGLPILPESKIGPGTRVRIRSGALAGIEGLVISRHGEN
                     RFIVTVSFIQRGASILVDLLDVEPVETSDAGRSA"
     gene            20415..20834
                     /locus_tag="PLD05_07435"
     CDS             20415..20834
                     /locus_tag="PLD05_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOP77307.1"
                     /translation="MCHELSQQPLTDATTSLSQLRQLVAEFIRARAWEPFHTPKNLVM
                     ALAIEAAELMEHFQWLTPEQAEEIKTDQAKRSEVADELADVLCYVMALANRLEIDLSA
                     AVHAKMLKNARKYPVEQFRGYFGPEDPRWPGRTSTEH"
     gene            21080..>22866
                     /locus_tag="PLD05_07440"
     CDS             21080..>22866
                     /locus_tag="PLD05_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOP77308.1"
                     /translation="MKEALEELQQAILAHVRSEKYQPVKPRVIAKALKIPAEEAHLVK
                     KAVKALVKAGFLEFGSGHLVYLAGTRKSAKPIEQASGIIGVFRRKEAGHGFVRPKPQP
                     GQTEPLEEIFIPPEWSSDAATGDLVRVRLAKRKRGRDLGPAGQIVEILERQSSRFVGT
                     YFEADGQGYVQVDGTVFAQPIYVGDPGAHGVQPDDKVVIEMLRFPNHFRQGEGVIVEV
                     LGPLGKPGVDTLTIMREFNLPEEFPPEVLEEARQVAQAFEETIPPDRYDATAETTITI
                     DPEDARDFDDAVSLERLADGSWRLGVHIADVAYFVPPGSLLDHEARNRGTSVYLPDRV
                     IPMLPELISNGLASLQPGKLRLTKSVYMEFTSEGIKTDVKIERSVIRSDRRLTYEQVD
                     AFFEDPAAYKRAWGTKVHDLLLRMRELARILRQRRRDRGALELVMPEVKVDLDRRGRV
                     VGAHLVPNTESHQIIEEFMLAANESVAETLRDRGWLFLRRIHRPPTARKLRQLQDFVE
                     ALAFPSRDLEDRFGLQSLLEITKGKPQQYAVHYAVLRSLQRAIYSPLEEGHYALASEC
                     YCHFTSPIRRYPDLTVHRLVDAMLLNFKPR"
BASE COUNT         5491 a         6696 c         6069 g         4510 t
ORIGIN      
        1 tccaaactgt gaaggacctg aaccaggggc ggcataaagg ggtaattcgt ctcgcccggc
       61 gagaatcgga cggaaggtta ctcgttttcc ttcgtcccgt cttgggcggg aagcaaaccc
      121 catcggccaa gcagccatcg ctcgatgggg cgtttccagc gggtcaccca gggttctttg
      181 atcccactgc gaggctggac aaggatggtt ctcaacccgg cccatcgccc ggccaagacg
      241 tcagcaaaca attggtcacc caccatggcg gcttgctccg gcgaaactcc aagaagcttg
      301 atggcccggc ggcatcccca gggaaagggt ttcagtgagc gtgcgacgaa tggcattccc
      361 aaatgaccgg ccagccgttt gatgcgacgc ctccccccat tcgacacaag gcaggtttga
      421 atgcctgcct cttgaagttg gcgaagccac tcggccacca cgtggtccag ctcgccccga
      481 cggtaatcgg caatggtgca gtccacatcc aaaagaaggc aggcgatatt ttctttccgc
      541 aagaaaggga tgtcaatctt cgttaccgac gagaaatagt agtccggctt tgccaagaca
      601 cccatggatg ctcctcaacc tgcactgtcc caactgtccg cttcagttgc gccattgcca
      661 gaggatttgg atcaagcgaa accaattata cgggttggca agccgaaccg cgatccgtgg
      721 ccaatcccgt cgggaggtga gcatccgtcg tacacgggcc ctctggggga taaagatttc
      781 gcgtggcgga agactcagtg actcgtcctt caaacgtagg caacacgcac atccctcgct
      841 cacggcatcc gccagatgcc agatggcctg attcaggatg acggctgccg tccgcggatt
      901 gatccgagac tcgaagcgat tcaattcctc ccaagctttt tccgcaaacc agatggcttt
      961 ctgacgatat cgagcggccc tttctggctc ggcaaacttg gccgccaaaa agaggaccgt
     1021 ggccttgcgg aactcctggg ctgcccaggc ctccgtggga tactccaatt cttcgggatg
     1081 gtccaggtat ggccgttcgt gatggaccat ccaatcgcaa tagcagatca gcgaacgccg
     1141 caggtattca aacgtgtgat catgttcccc aaaaagacac ttgatccgca gaaatttaca
     1201 cagcacttcg agaaacatgg tgtacgacca ccgggtctca gcatgaagca ggccaaaggc
     1261 agcgggatcg tccttaggat ggatgacgcg tcggcaaagc ttttcagccc agcttcgata
     1321 aaccgcgtta cccgaaatta accatgcatc gagcagggca ttcacggaat tggcggagcc
     1381 acgtcctgga ccatgataat cgggcgaact tgtggccgtg gccagcccgg tcgggccatc
     1441 gtccgcaatg aaaaaaggtg tctgccgccc gtcctccatg gaaatgaccc aattggcaag
     1501 agataggacc gcatcccgag cgtcctcatc tccccagaga aggtaataga gcgtcaaacc
     1561 gctcgtgtag ttctgctcgt tgctgggccc accgccgtaa ccaggccgct gggcgttttc
     1621 ccgcgaaaaa gcgcgatgcg tggccgtggc cgccgtgacg taatgatcgg tcatccagaa
     1681 catgccgcca ttaaacgtgc ttcgatctcg caatgtgtgg tagatgtcaa tatcgataac
     1741 atggcgtgcc agcggatgaa tcagctcttc ccatcgcgga tcgttcgaca ccagtcggca
     1801 cagaatcaac ccgcataaaa gatcgaactg attgttgtaa tgcgagacaa acggcagcct
     1861 cccctcatag aaggcctttt cgtgcgaagc gtacacatct ccgaaatgcc gccagccgta
     1921 ttcgtcgatc tcttcccggc cggccagtag ccccttcggc ccgaagccag cctcttcggc
     1981 aaattgggcc aacgccgtgc ccggccaaag ctccggaggt ggtaagtcgg aagggagttc
     2041 cacaagcgct tgttgcaatg cagatcggac cgttactcga cgacacaaag ccattaactg
     2101 cgtcgtcacg gcatggcgac cgtcatcgct cccatcggac aagacgatcc aagccacgta
     2161 cgtcttttgt tcccctccct gaagctcatg cacatcctca ggattctccg caaggaaagc
     2221 gagggtgctt gatcgtgggc ccacatcaat ccgtttggga aactcctgcc aaaagtcggg
     2281 catccagaac ccgcaggaat aggccgctgt ttggaggcag gccaacgggt cggcgcggtc
     2341 cccagatgtg gtgccctccg gcgaaatagc gcgataacca cggaacggaa gcgtcacttg
     2401 tccccgtgca ttgacgtggt tgaagctatt ccagtgatcc cctccgctcg aggcttgatg
     2461 cacttgccaa atcaatgagt caccgtcacg agaaccggtt gagtttttcc ccggccacct
     2521 gtcaccgtcc acgatatgga acacctcgga tggcttggcc cgccaaacga ggtgcttgag
     2581 cggatcgcgc cactccaggc gaagaccgaa ctcccgcaat aagattgatc cgggatcgcc
     2641 taagtcccac agcccaccac ggtggcgagc acgccgggta ttgcgcagca tggccttgat
     2701 gtgaatcgca ttcagcgtgg gataaaccct cagcgtcact cgccataaca gtccccttac
     2761 ccccggaaat gtcccctccg ctgtggcctc ataacaaatg ggtccctgca cgtgccatcg
     2821 gagatgacgg cattgcccta accgcacgct tccagctttc gttttcatga acacgtgggc
     2881 cactgccagc ggctcattcg aagcgcggtc cacaaaggca aagacggtat ccctggaaga
     2941 agttggtacg accgagtatc caagtcgaag tggaatacgg tcaggtatgc cctgcccggt
     3001 tggttcatcc aaggactcga agtcgagctg ccaggtctca cgccattggc ttgtgaggca
     3061 ggcgtgaatg accgtgtcaa ttcgcaccca gcgggcactt ccgtcaggcc aactcgctac
     3121 gggagcaatt tgagtgggaa ataccgctcc actcggagaa accaattgac ctcggcacgg
     3181 atagctcaac ttccctcgcg gcaacggcaa gccaaagcgg agcggtactc caacgttagg
     3241 caaatctacg ggagttcccc atgtgccgag atcaatcggt atcgacaagg tttttgaact
     3301 gtttgccatg atgctgccct ttaagacgtt tccacgccga tttcgcttga cgccggggat
     3361 aatcgtcgga tgacccgggc aggtacaccc gccacaacgg aatcgtcttc cacaggagag
     3421 gtcaccacgc tcccagcccc cacgatcgcc cgcgttccca cgttagccag aatcacggcc
     3481 cgctccccga tccatgtcct ttcgccaatg gccacccgat catattgatt cggagattct
     3541 ggtcccccgg catcgccacc ttgaccaacg acttgcaccc aatgctgtcg gctaccactg
     3601 gggatggaca cgcccgatgc aatccggaca ccgttgccaa tttgaacatc cccaagaacg
     3661 cagtatgcgc cgatatagac gtgtcggcca atctcaatat tgggtgatga aaagatcgta
     3721 ccgaattcga tggtcacgtc ccaactgcac cgccgggcgg cgaggaaata aaaactccat
     3781 cgcaccaagc aaccacatcg tccgggaagg agagaaagac actgcgacca acacgcgaag
     3841 gccgggcgtt ttccccagat tagggcttcc agacgataaa gcacccagaa caacacgccc
     3901 agggagagaa ccagcacttg aacacatcgc ttgatgataa gccgcgggct cacagcttct
     3961 ctattcaccc cattttctgg aggatcactt gacatcgagg atgccgatac ctctaaccta
     4021 aatgagcgta ggtcgccgtt ctttcccgga agattactta ttcacgattt tctcgctcga
     4081 aaacccttcc aacgggcata gaggctccgg agcttggcga cgtcacgagg tgtcagaaag
     4141 gttacgaacc acaggcacaa ggaacggaga tgcggttccc aacggaatga ttgccgaaag
     4201 gcccagcggg cggcacgatg gtcgtgataa tccagttcat aacttcccag gagatcataa
     4261 aggcgggcca gccgccgcgc cacggaatgg cgcagtgccg atggtaaacc tggcagtcgc
     4321 cgctgaagga ctttgatctg ttcgcggagc atcattcggt ggttgctcgt cccctgatgc
     4381 gaatggagac gaaagaccgt caacggttca tcaacatagg caaacggcgt gacctgggac
     4441 aatcgcaacc acagatcgta gtcttgaacc gtgttgccct cgatgatggg atcacacagc
     4501 cctacctgcg cgaaaagttc ccgccgtacg aggacactgg agttgcaaat cgcattgcca
     4561 aacaggagct tttcgaaaca ccagccgacg tactcctccg gtttggcctc cggatcaagg
     4621 ggtgggcaga acgtctgtgt cacatcgtgg aagtacacgg tgtgggtgtg aacaaatcca
     4681 gcgtcagaat tcgccagagc ggccaattgc cgctcaatct tttggggata ccaccagtca
     4741 tcctgatcga ggaaggctac atattcaccg cgagcggcca agattccccg attgcgcgcc
     4801 gcgcccaccc cgcggttcac ctggcggatg cagcaaatct tatctccgta acccgccagg
     4861 atctccggcg attcgtcggt cgatccatcg tcgatggcga tcacttcgat cggagaatag
     4921 gtctgagcga gcacactgtc gatcgctttg gcgacatact gggccccatt gaaaaccggg
     4981 atgatcgcgc tgacaagagg cctactcata tcacgaccgt tgctgttgtg tggcgacaag
     5041 actctcaagg gcggccacca tccgagaaga aaagttctgg ggatggaatc gctcgagcgc
     5101 ccaggttcgg gcacgctggc ccatttggcg cgccaattcc ggttgggaag ccagtcttgt
     5161 cagggctcgt ccaagggcat ccggatcctg gcaagggacc aggtaaccgg tctctccatc
     5221 gcgaacgatc tcttcgggac caccacaagc cgtagcgatg gctggccttc cacaagccgc
     5281 cgcctccaca atgaccagtc caaacgactc ctccacagag gtgagtaaga gcacgtcgct
     5341 ggcaaggata atcggctgaa catcccgctg gaaaccaaga aaccggagct ttggcctcaa
     5401 ctcctcaggg acgagcgact caacggtagc gcgtagggga ccgtcgccta ccatgagaag
     5461 aattgcaggc cgtggactgc gcattcgggc aaacccctcg atggcaagct ggggattctt
     5521 ccggggaatg aattgcccaa tccaaacgac aatcaaggca tcctgcggca gtcccaacgt
     5581 attacggatg actgcgggat cgtgagatgg ttcgatatcc gtaagaaaac gcacgggatt
     5641 cgaaacggcg tctgtttttg tcttgggctg atgcaggctc gcaaaaatgt tcctggctgc
     5701 ctgcgattcg aacacgataa gatccgacca ccgatcaatc aggcgacaga tcgtgcgaat
     5761 cggaagccaa cttcgccacg attgaggccg taagatttca tggacatgcc agaggtgggg
     5821 acagtgagct atccgcgcag ccaaggccgc ttcgaagatg acggcagaat tagtatagac
     5881 cacatcaaag gactcctggc gaagcaatct cgccaagcgt gcgacccgaa aaggtgactt
     5941 gagaagattc cgccaatacc aggagcaggg ttcatatccg aaccaccaaa caaagggaac
     6001 cacttgggtc ggatagcctt tctccgcggc ggcctgcacc atggggcctt cacaaccaaa
     6061 caagaccgtg gctttatagc gagcacgcgg aagatgtgcg aggaaaacat ccaaacacaa
     6121 ctccgcccca ccctgctggc cggaatgcgc cgcaatgaaa atgcgaagct gacgactctc
     6181 atgagcaatc acttctcttg ctggcgtctg taatgccatt aacgatcacc ccgagcccga
     6241 atcggcatca cagcgagtga gagcatcgaa ccaatctttg actcgataga caagtggcgg
     6301 aaagatgaat tccctgagaa ccggtttggc ttccgcggga agtgggccct tccacgatgc
     6361 caaaaaacgg cgaagtgccc aatactcctg ccagttgcga cggtgttttg ccagggttgc
     6421 ctgttctgcc atcaaagcta agtattcacg ttgaacgagg tctttctccg caacggggag
     6481 atcggcaacg ccctccagaa actgctggag ggcatggaag cggcgaacca acatgtccgg
     6541 ccgcgaactt tgaccctttg catggacgcg atagcccgtc acaaactccc gaaccagtcg
     6601 aatctcgcac tttcttgcta attccaaaaa atagatcagg tcttcgccat cttgtgtcca
     6661 ttctgggaag cgaatatcca gatcgcgacg taccagcagg ctcgaaatgc gaaaaggatt
     6721 tcgccgtgct acgtaaccta gggaataccg tgtggctggc gggacatcac ccacatccac
     6781 ggtcccttgg ctggccccaa aatagaagaa gttggtatgg acgcaatcaa cccccgagta
     6841 aaagagcgct gcctgtaatg cgagcttttc tggcttccaa acgtcatcgc aatcaaggaa
     6901 ggcaatccaa tctccttggg cagcgtcaat ccctcgattg cgagcggcag cctctccccg
     6961 gttctcttgc cggatcacgc gaactggggg tccaaacgat tccgcaagtt gggccgatcc
     7021 gtccgtcgat ccgtcatcaa tcaccaagat ttcgtacggc ggtaacgtct gggaaagcgc
     7081 tgacttaatc gcctcgccca aaaagcgctc gccgttaaaa acggggatga ttacgctgat
     7141 ccgcggaaat tcacggggcg gtcctgaagt ttctgccata gcaccggcct accgaattga
     7201 ctcggtcccc gttcaccaag tgtatcccag ggcctcgaat tcccgtccgg caatccgacg
     7261 gaattgccag cgaacccacc agggccaatg tttccaacgg ccaacaggct gaaaatcctt
     7321 tcctttttca acgggttgcc agtgaagctc ctgacgagtg ccgcgataaa agctcgatcc
     7381 ccgcaccggc agattctcaa accgatccca aggaaacgta ccaggatcgc agccgagaaa
     7441 cctgatgacc cgttcgacct caccacgatt ggacagaagt tgttcatact gtgtgtagat
     7501 cacctggccc acgtgggatt gtggaagtcg ccgaaagaaa tcgagaattc gctgggcatt
     7561 ctgggcccag gccgtgatcc agtaattgta cgaatgaccg ggcagccctc gtttcgacga
     7621 ttccacacaa tcgcgtccgt cacggacaag agcgatgagc agcgcctcgg gaaagaaaag
     7681 aaagaagtcg ttcaaattcc agggatcggg ggtctttgtc agaaggcgtt ttccctcggg
     7741 caggtattgg cttagaaacc ggatcaagcc gtcacccaag tggcgatgca acgaagcgac
     7801 gcatcgctca tactcggcag gggcattggg aaatcgtttg cgatattggg cctcggccgt
     7861 ctcgacgacg tattggcgaa ggagcgggga atactgcaag acataatcct cggccaacgg
     7921 cagtggcagg gcaaattccg gcaggagaca aatggcatcg gcgaggaaat tggtccccga
     7981 acggcgatga atcccgataa ggaagacagg tcgcttccgg cgatcgccgt ctgccagcca
     8041 aagcgattgg aattcgtcag tcgaaaacag gcgaaggtgt ttatccatag aagcggagtg
     8101 agtcatgtct caagaaccat gtcacgtggg gcggtagcgg cgggcttgtt tccccttcat
     8161 ccagcccaga aaaaaccaca gctcataggc ggcctgaaaa cgccgatgga cgtctcggga
     8221 aaaacgggtc acgcaccagc ggctgaatcg ttccatcaat cctcgatagg cccaacgggg
     8281 ataaccgaat agaaagactt cactaccggg ttgctccaag ccctcagcaa caccaatctg
     8341 atacgcccga cgcaaggccc attgcggcga acaacgtgat cgcggcaccc agtgataaac
     8401 aagtccctga ggaagataaa cgggtctcat tcctcgttgg aagagcttct tttgtaaagc
     8461 cgtttccgct ccgacgcccc ccggcccaaa ttggttctca aatccgccca cagccagcag
     8521 gtcatccgcc caggccgccc agtttgcacc gagaaacatc ggcaccgttg ttgccatatc
     8581 ctccatattt ggttgccaac cccgcgctga ccgcgggaga taggtaagaa gccattccgg
     8641 gggcggcgtt tcgtagtcga tgccaagtgg cccaccgtag tactcgcgcc cttgttgccc
     8701 tctttgggcg gctaagtggt aggcctcaat cgcctccgca gtgacacgca catcatcgtc
     8761 taaaaaccag gcaaacgtcc ccggattgcg atttagaaac gcgttagacg ccgctgcttg
     8821 acctacctct gggatggcca ggtattgaat ccggcaaccc tcggccaaag acttcaccag
     8881 ctcggcagcg cgccgcgtgc ccccgttttc cacgacaacg atttccaccg ggaatcgcga
     8941 aacaccagca gtcacaagcg agtgtaaggt ccgtcccaaa agatcggtac gtcccaccgt
     9001 gcgaatgaca ataaaccagt tctggtggtg tgacatcatt acgcccgatt tggaaaatcg
     9061 ttaaccctta taggtgttgt gtctcgcccc aactccggta ggcagccgat tttcgatcat
     9121 cgtttgattg cccctacaga aaggcacttc gaggcaatcc ttgtctgggg ttataacccc
     9181 aaaggacaga gagccgtaaa gttcgcgatg cccagcggaa tttgaggaaa gcgcccccgc
     9241 tactgaccgc aatatttgat ttttcccatt gtggttagac ggcatcgatt gtgcaaacgt
     9301 tcgtcgccgc aactactgac agattctttc gtacgttgac accacacact gggcaatctt
     9361 tcgccgctcg catcgttcgg caatgacctg ctgattaatt gcggccgcgg aatcgacaag
     9421 gtggtcctcc cacaaggcgc ggcggattgc ctctgtcaca gcgtgtgggt cttccggcgg
     9481 cacgatcagc ccggtcttgc catcttcgat ccactcatag gccgcaccgg tgttcgactg
     9541 gatgggaaac gaccccatga gcatggcctc gagaagcgaa atggcgattg aatcgctaat
     9601 gctcagccca atggatatcc gtgattgccc gtgcaatcgc aacatggcct catggggcgt
     9661 ccgcggcgga acaaacgtga ctgggatatc caggtcctga gaaagcaatt cgccagcaat
     9721 tcgcaccggc tccggatcac gaccgcccgt ataaacggta atgcgatatc cggctaacag
     9781 gtcgctacat ccccgcaggg cctgaaaggc aacaagggcc cggccagccc aatcctggta
     9841 acccttgacc gtgatcactc gccggctcga cgtcggaccc ggcgtccgta gtttttcggc
     9901 ctgctcaata tcaaaacctc ccggcgcagg caccgttggc aagatgcgct cgctgggtac
     9961 cccccaactc ttcgccaact cgacatcgcg ttgacattca caccaatagt aatcggccgt
    10021 attgaggacc ttcgccaacc gctccttgtg ttctgtcagt cggccaaaaa gaaacaagtc
    10081 gcttccccag ttggtcacca cccacgtggg aaagggtttg cggcactcct ctcgcgccgc
    10141 caaagtgaga tagcctccca ggtgcaatcc tagggaatgg acaatatcag gccgaagccg
    10201 gtcgatcagt cgtgccaggc gccaggcgtg attagcgaat tgcgttcgac atcgcgtgat
    10261 ccattggctt gcccgatagc ggccaaacgg caagggccaa tctcggccaa acgccgcgta
    10321 gaaccgatca tggaacttca ggccaggttg acctgtccat cgtcgtgggt cttccacatc
    10381 atgaatggtc acacccttga gctcagggcg aagatggtaa agttccgtgg gaaaaagatg
    10441 aaggtcccaa ccacgatccg tcaactggtc gatccatcgg gccgtatgaa tgcttccacc
    10501 aacggcgaca aagagaattc gcatgatcct tgctcgttaa ctcctcaaag ccatgaatga
    10561 ttaccaattt ttgtgaggca atatcttcca aaaggctacc caacctcgcc gaagatgttc
    10621 ccaacggcgg tgtcgtcgcc agacctcata aggaatcggt tcgccggaag aaagcaaaag
    10681 gacccagtca aaccctgtgc aggcaaccaa acgatggggt acgtccataa agtccctggg
    10741 tgaccaggtg ctgacgtggc gttcgtaggt attcccgaac acggccccct gttcccgaac
    10801 ctggatgggc gtggaaatca agagaaaccg tgcctttttc atgatcagtt ccaagacacg
    10861 ctgggcatag tctctttcaa agtgttccca aacatccagg acgaccacga ggtcaacatc
    10921 ggggagcgtg cccaagacag agagggcatc cccaatccgg acttcgtcgt aggcgtaatc
    10981 ccataaggcg ttgtgatatt ggggaaaggc ctcaatcccg taaatcttcg tctgccagga
    11041 ggccttgtca tatcgctcgt tccaaatgtc ggtatattct cgaatcagga caccagtctt
    11101 gccgtggccc acaccaatat caatcactga tcggggcgca agtcgcagga tgatgtcatg
    11161 aacgatggaa agcgttcgtg ggtcgctgct gggcatcaat caatccttgt ccagtcacag
    11221 gagggcgtga caaggccaac cgtgctcatg atatcggatc cccggatggg gcgatcctga
    11281 tacacttcga actggaggac ctgccggtgg tgaaagaact cacggactcc cctttcggca
    11341 aggaccagac cgacgttgta ttttcccgcg ggcaacagct ccgcaggaat ccgacaggac
    11401 aggcagaacg gtccgggccg gcccaagatt gcgtaggatt gatcttgttc aaaactatgc
    11461 gtccgaaaaa gtgtcaaatc gtcgatggtc ttaatgtcga tagcaacgat taagcggcga
    11521 tccgcggcac ggacctctcc tgttatctcg atgacgatcg gctgactgcg aggaacaacc
    11581 ggggcgggcc gaccatcctc ggtgaccaat cgaagccgat ggacctcgaa cggggcgtcg
    11641 ggcaggcccg gaagttcaca cacccacgat tgatactggg ccaggctctg gcggagatag
    11701 gcatccacga cctgttcggc aggcccttcc tgggtgatcc gtccgtgctc caagaggatg
    11761 acgcgatcac acaactgcat gaccgcccgc atgttgtgac tgacaaaaat gactgtccgc
    11821 ccctggcccg cgacgtgttg catcttcccc aggcactttg cctgaaaggc cgcgtcgccc
    11881 accgccagca cctcatcgac cagtagaata tccggctcaa gatgggcggc cacggcaaag
    11941 cctaatcggg cgaacatccc ggagctgtag cgctttacgg gcgtgtcgag aaatggtcca
    12001 atcccggcaa attccacgat ttcatcgaat ctcgccttga tctcggccat acgcatgcca
    12061 aggattgccc cgttgaggaa gatattctcg cgtcccgtca aatctggatg aaatcccgcc
    12121 gcaacttcga tcaaggctcg gagccgaccg ttcacgcgaa catatccttc ggtcggccga
    12181 aggatgcggg caagaatctt caggagggtg ctttttcccg ccccatttgc cccaataatg
    12241 cctaacactt cgcctcgccg gacctgaaag gagacatcgc ggagggccca gaaccaacga
    12301 tcagtgggct gagactgacc acgggagaac cctaaccgac ggaccaatcc cggaatcagg
    12361 tcgcggaggc tgtcatagga ttcgccgcgg cgaaacttct tgcttagatg ctgaacttct
    12421 accaagacgt cagatgacat tgctagccat ggatgactct tcgggccgga ccaacgataa
    12481 cggccgggca acggcaggcc atctcaaata tattccgcaa agacgaactc actccggtgg
    12541 aaaatcaacc aggaaacaaa aaagcccaca acagccacaa nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn ccaatgtggg caaacaccac cagcaggacc gacaagaaga
    12721 tactgcccac gagggtatca aaaagttgcg tgaggaccgc ggacaaggga aacaattccc
    12781 ggggaaaata gaccttggtc acaagcgtca tgttcgagac caaactgttg gccgcaaatc
    12841 ccagggcacc cgcaaaaaac gcccagccaa gggccttgac ggacatcccc gccacacgcg
    12901 gcaaatcaac gccatcgcca ctcgcccggg cgagggcaat tttcaccaga caaccggcac
    12961 cgacaatcaa cagcggcatc agtaatgccc agccaaaccc catcaccgcc tgtttataac
    13021 ggatacgaat atccctcagg accatttgtc cgagaagttc ccggtactgc cataattcct
    13081 gaaccatctc gcggaacatc ggcccgagac gacgcgtatc cgacgaactc cactcccaac
    13141 ccaattcgtc ggataccagc ggtgatctct ggggactatc agcttgccgt tgttccatgg
    13201 gagggtctgc tgggttacag gacatcgagg actatgggag gctttcttgt tcaggcaaat
    13261 cgcggccttg atccggaacc ataaccggaa tggtcacgga aggcagggaa cgaggcgtcg
    13321 gcggggctat caagcggggc tgccacagct tccagggttg gctctgccaa aacctcatga
    13381 cggcccatcc acgtgggtgt gtggactcgt agcaaggccg cgcccaatag ggcagtataa
    13441 taggggattt ccagccacat gacgctgata aagctcaccg acacggcaaa gccaattaag
    13501 gcggcaacga ccccccgtgc catgttctgg gaccaaaggt ccaaatgggg acactgtcgg
    13561 atcagtgtcc aaaggcgaaa gatggtcaag ccatagaatc cgcttaacaa gagcaggcct
    13621 ggaatccccg tctccgcgcc cgtctccaac caaagggaat gggcgtgcat cttgggtaac
    13681 cccaagcgct gcgattgcag aggccagtga ttgaccccga cgcccatgaa agggttgtcc
    13741 cgaatggtct ggagacacgc cttccattgt tcgacccggc ttcgcgtgga ccggtcacgc
    13801 tgctccggat ccacaaaggt ggtcatgaat cgctgacgga cctctggccc ggccaagcga
    13861 agagccaaaa taattgctaa tccatagatg agggtcgtct taaatgtctt gggaattaat
    13921 aagaaggcgg ccgttccccc tgcgatcagt cccaggagtc ccccgcggga aaaggaaaac
    13981 ataatggtat gggccatcaa gccgcaggcc gcaaaggcca gggtcttcag ttcccagcgg
    14041 gactccgcta taccgaggaa aaacgccagc aacgtcccga ccaccatggc tgccgcgatg
    14101 caattgttgt caaacccgcc ataagcctcc agcacaatcc ggttgatccc ttggtagtag
    14161 ctgcgattaa aatccagggc caaataaccc tgtgtgatca caataaccca tgccagccaa
    14221 cgaacatggt tggttgtctg aagcaaactg atccccagca ggaatggtag aacgattttg
    14281 agtagcgaaa tgataaagac cttggcaagt gccggttccc agcagaagaa atatcccaca
    14341 gagccccagg ccaaaaatcc caccaggcac cctgccacgg ccgtggatcg tcccaaccgc
    14401 caatccccaa aaccacggat ggcccaaccc accagcatcc ccacccccac aataaagcta
    14461 tagcgccccg gtccttgtgc cccaactgac cagtaccaaa gagactctgg cttcaaaatc
    14521 gcatagtgga tgtagataaa aaggcccacc aagggataaa aaagcgatac gacggccccc
    14581 agataggtca tcaaatagac gtacaccaaa ccgcgcatcg ctaggcctca gaagatgtca
    14641 agttcgttcg caagtcgcat cctttatgct tgcttcgtcc ggtgaagaat tgtctcaatc
    14701 gctccaccag tagcgaagga cgcaccacac ggcctggagg gcatcccgaa aaccgatctt
    14761 tttcccctgt tgataagtcc gcccataata actgatgccc gtctcgtaaa tccggaagtg
    14821 tctgcgagca accttggccg tcagctcgat ctcgattcca aaacgatcct ctttgagggt
    14881 gggcagaatc tccttgatga cctcactgcg aaaggccttg tggcaggttt cgacgtctgt
    14941 caaattcaaa tccgtaaaca tattggagag cgtggtgata aagcggttgg cctggtagtg
    15001 ccaaaaatag aggacacgat gcggtccttc ggacacaaac cgtgatccgt aaaccacgtc
    15061 ggccacacca tccacgatgg gttgaatcag ccgcagatgc tccgcaggat cgtattccaa
    15121 gtccgcatcc tgaataacga taatttgtcc cgtggcttgg gcaaatgccg tccgcagggc
    15181 cgcgcccttg ccacgattcc tagagtgccg caagaccttc acctcgcttt gcgactccca
    15241 gcgacggagc cgttcactcg taccatcggt gctcccgtca tccacaacga ggacttcctt
    15301 ctggatggga acctcgagaa ttcgctgcag aattgtgtcg atcgtttcca cttcgttgta
    15361 cgccggtacg accacagaga gtcgcaaatc ctgtgggatg tcatagatcc caaaacgttt
    15421 tcccagcgtt tgccacagag cccaacggcg gcgatccacg cccgatgtca cagcagatcc
    15481 gcaaagggga caacaatcgt cagccataaa cgggatatga ggtctttctt ccctggttct
    15541 cttttaccga attcgccaca atcaattcgg tccttacgag agtcccaaca aatcatcgag
    15601 tacccgaagg cgatcgggcc attggtagcg cttctcgatt ttcgctcgcg cagccgcgcc
    15661 aaggcggctt cgcatctccg gtgaatgaat caactgtgtc aattgctcca cccagttgtc
    15721 aacgtcctgg gctgaaagga catcctcccc aacggccaca tccaatccct ccagggctgc
    15781 gggtgaggcc accacaggac agcccatcgc catggcttcc aaaaccttgt tctgcaaccc
    15841 gcgagcaatc cgcaacggga caacgcttac actcccgcga agatacggac gcacgtcggg
    15901 cacattaccc accagtctag cccccggggt cgcttcaacc agacgccaaa aagacttccc
    15961 cgcccccgcc ccgacgatgt caaacaccac gtcacccgcg tttcgtaccc gtggccagat
    16021 ttctcgaagg aaccaagtca cgccatccac gttgggataa taatccatcg ccccgacgaa
    16081 aacgagacga accggtgttt gtggcgccgg cgttggctcg ccgctatgag cgggatcgaa
    16141 gtaggccaaa tcgacgccat tttcgacgac gcgaatcttc tccgtgggat gaaattcgcg
    16201 aaaaatctgg ctttcctgtt ccgtaacgaa gatgagaaac ttcgcaaggt gatccagttc
    16261 acgttcaaag cggcgaaggc ggtttccctc cagacgatag agaaacgcct gcggaaaagc
    16321 cactgtctcg gcgtagtcga gccacttttg actgtccaca tccacaagat ccacaatgca
    16381 acgattaggc gacaagccga gacgcctcag gtagatggcc atgctagaac aataggcaat
    16441 gacagcatcg taggtggtct cggcaagcca tcggcggacg gtgcgttcca accggggcga
    16501 gtagaaagcc gcctccgtgg cactgcgtcc acgaaggagc gagagtatcc ctcgcatccg
    16561 ttgtcccaac cggctagtcc gaacccaggc cacacgttga caccggaaac gaagctccgt
    16621 gaggtggtct tcttctaaag gttcgtccgc caggaaggcc aaatccacct ccgcccgttg
    16681 cgaaaggtag tcgagccaat ggaatgaacg aatgcgatgg ccccggttcg gcgggtatgg
    16741 tgtgcggtgg accagataca agatgcgttt gcgactcatg gccgccactc tagcccgcgc
    16801 cacagtgcca cacatccctc acacgaagta caccaacacc acggactaca ccaatacggg
    16861 gtggggacgg atccgcaatt ccttcgaaaa gccggcggcg ctgccacaga cgttctcacg
    16921 acttaaaggc gagtgatgcg aacattagcc ccgcgccaga gtgctcatcg gaacaacatc
    16981 gtcggggtca acgccctggc tttgccagct cgcaagtgat tccctaagag aaccccacga
    17041 aaacgctcgc aataaggccc gtagtttttt ctccgtcgta gcaaggttga cataatgccg
    17101 aaaccgactt actggggaga tgccaccaat gcgtggttgg tcgggatcaa gttcccaagg
    17161 atggacgtac atcatgatgg gtcgttgctg ccgaatcaca cgttggaaaa gccgccgtgt
    17221 cagccaaagc ggatacaacc gcaaatatcc cccaccgccc agcggcaggc ggagacccat
    17281 ccaggatagc gcagtgccgg gaaactccca aataattccc gagtccgtgc ggatgcggtg
    17341 cggttccagc cgagcgtctg ggattccata acgatcatgg acgatcggat aaatactagc
    17401 gtcgcaggtg atgccctctt cggcaaggat ttcgatcgcc caaagggacc gacttgtgat
    17461 cgaaaaactg ggggcccgaa agacggtgac acgttcgccc gtatgatcct ctaacagctt
    17521 gagcgactgc cgcaaatctt cgcgaaactc ccctgggctc agatcataaa ccaggcgatg
    17581 ttccatgcta tgggagccaa tttcgtgccc catggaatgg ataagccgga tcaacttggg
    17641 gtactttttc gcgatccaac ccaggacgaa gaatgttgca gaaacaccat gctccgccaa
    17701 aatatccaag attttttcta cgaatggacc aactcgactg ggatattcgt cccaccgtaa
    17761 gcgagagaca accctttcaa aggccgagac ctgaaagtat tcctcaacgt caaacgtcaa
    17821 ggcatggaca agtggcaagg gcgtcgcctc accgcggttt tctaaagata ttgcggcgcg
    17881 cctccgcaaa atggatggtc ttatcctggg aacggcgctg taattgcgaa tacgtgcacg
    17941 ggatttggtg actccccgtc ttgccgttga acatccatgg tgggattcgc gccggacgaa
    18001 gcaagccgag ccaaccggcg atctttaacc ctggcaggcc gaccatcttc gacaccgtac
    18061 ataacagaat tcgaacatcc agccaaaaat tggccttttc gatgtactcc agatcgagaa
    18121 ccagcttgcg gcgcaccgaa tcgagatcgc tatccggcgg caaattgatc tgagcaagtc
    18181 ccgtaatacc cggcaaaacc gcgatgcgat tgagataacc gggaatggcc tgggccagca
    18241 tctgggtaaa ctctgggcgt tccggtcggg gaccgatgag cgtcatttgc cccatcagga
    18301 cgttgaaaat ctgaggaagc tcatccaaat gcaaacgacg caaccaccgt ccaacgggcg
    18361 tgatacgagg atcgttctcc tccgtccaga ccggccccgt atgagcctcc gcatcgatcc
    18421 gcatggttcg tattttgtag atccaaaaga tcttgccgtt tttgcccacc cgctgttggc
    18481 gatatagacc gggtccccga ctcgtcaacc gaaccaatag gacgaggaaa ccaataatag
    18541 gcagaaacac cggggtggcc agtgccgtga gtatccaagc ccaaatggcg tgccagcgaa
    18601 aatacggcga cggctgaatc ctcggtacgc cggaacttcg actttttcca ttttcatact
    18661 caacgcgagc actacgctta gccgcggatt caccatgcac cagccgactc atcgaagcct
    18721 ccgcgacccc aaatgacgac gccgaatcaa atgatgatag ctatctttct cgcaccattt
    18781 acccttctgc cctctttttg atatccgaag gattcacact attaccgtct cataacctaa
    18841 cccgattgtc cggcaatgtc aacaattttt gcaaattcaa cacatccgac aatgattacc
    18901 aactaaccgc gcatccgcgt tgcacaaaaa agtaacccca atggttgaca actgccgcag
    18961 aaaaatgagc cggcaacttc aagcggaacg gccggcgtct gaggtctcca ccggttcaac
    19021 atcgagtaaa tccacgagga tggaggcccc acgctggata aagctgacgg taacaataaa
    19081 gcgattctcg ccgtggcgag aaatgacaag cccctcgatt cctgccaaag caccggagcg
    19141 gatgcgaacc cgcgtccccg gcccgatttt ggattccggc aaaatcggta aacccgattg
    19201 aacgattcgc caaatattct ggagatcctt caccagtcgc gggacgtcgg gcacctccag
    19261 gcaatgcgtg acgcagttcg tcgtcatcgc ctgatagcgg tcgtcctcct gcccacaaag
    19321 gaacacatat ccgggaaaaa gggggacatg tgatgtaagc gcgcgaccct ttggcgtgcg
    19381 gactcgcttg gcaaccagcg ggcaataatg ggcaatctcc atttcccgca agcggcggat
    19441 gaggaccttc tcctgccgcg gcttggtata aagcgcccac caggattggc tccgcgctcg
    19501 gtcgcacgga tccgaaagca gatcgtctgg ataaaggtct ggctcgcgtg gtcggatcgg
    19561 catacttgcc cagtccggtg atcgtcctga aaaaagttct tccttgtgtc atcatccgag
    19621 aaccgatccg ttttgtaaac ccgacctccc aatggagggg catttcctca aatctcaaat
    19681 ggctagcaat gaggtcaatt aacagcctat cccatgatct cccctcgccc gttcggcaag
    19741 agaggggtcg ggggtaaggg tggctcaaat ggccaccggt tttctcaatc gattccgacg
    19801 ttcgcttgcc ctcaccccaa ccctctcccg gagggagagg ggacctttta agataggctt
    19861 taagccgtgc tcattgcatc gcgaccagac tctgtccccg tgggttgcgc ccgattgggg
    19921 ccaacccaac cacaccgtac gaccacaaaa cactccggca gagccacaag cgatcgctcc
    19981 gatcaacggc tccctgtacc ccatttgcag accgattggc ctatgtcacc gtggccccga
    20041 gggtctccct cagctccttt tgcgcccgtt gttccacctt ttccgccgcc atatccaccg
    20101 gaaagacggg ggagtcacac tctgcatact ccccaagtct cctagtttac cgtcagccag
    20161 cacagatcgc caacactcag agaggattcc caaacaaccg gccgaacata ccaaggttat
    20221 cttctatggg ccaccaaacg cggcgagatt ccttgatgcg agactttgcg tcttcatccg
    20281 ccctaaacgc ccgcaagtcg cctcatgaag gtaggtccgt gcggggtgtg cggacatcgc
    20341 tccatgtttc cattatgatg caggggaggt gagtatccta gagtcgcatc atcttaggcg
    20401 tgggtattgt tgtgatgtgt catgaattat ctcaacaacc gttgacggat gctacgactt
    20461 ctcttagcca gcttcggcag cttgtggcgg aattcatccg cgcgagggcg tgggagcctt
    20521 ttcacacccc aaaaaacttg gtgatggcct tggccattga agccgccgag ttgatggagc
    20581 actttcagtg gttgacaccc gaacaggctg aggaaatcaa gacggatcaa gcgaaacgtt
    20641 ccgaggtggc cgacgaactg gcagacgttc tgtgctatgt catggccttg gccaaccgtc
    20701 ttgaaattga tctctccgcc gcggtgcacg ccaagatgct caagaacgcc aggaaatacc
    20761 cggtggaaca atttcgaggg tactttggcc cggaggaccc ccggtggcca gggcgtacat
    20821 caaccgaaca ttgaattatc acttgacgag cgtatattta aggaaagcag atgtcaaacg
    20881 ttgaaaaggg acttttccaa ggaatccccc aggctgtcca cgaggctggc ggccagatgt
    20941 gccctcgcat tgcgattgtc tcgccctgtc ttggagtgtc cttctttaag agacttaagg
    21001 tgggcgactt ctttgggagt ctgcgctctt ccagtctctg tgagcaattg cgcagagttt
    21061 tttgagaaag tgtgattaga tgaaagaagc gttggaagaa ttacaacagg caatcctggc
    21121 gcatgtgcgt tcagagaaat accagccggt gaaacctcgc gtcatcgcca aggcgctgaa
    21181 aatcccggct gaggaggccc atcttgtcaa gaaagccgtc aaagcgttgg taaaggcagg
    21241 cttcttggag tttggttctg gccacttggt gtatttagcg ggcactcgga agtcagccaa
    21301 gccgatcgag caagcgtcgg gaatcattgg agtttttcgt cgtaaggaag ccggtcacgg
    21361 cttcgtccgc cccaagcccc aacccggaca aacggaacca ttggaagaga tctttatacc
    21421 gccggagtgg tcgagcgatg cggcgacggg cgatctcgtg agggtgcgtc tggccaagcg
    21481 gaagcgtggc cgtgatcttg gtcctgccgg acagatcgtg gaaatcctgg agcgacaaag
    21541 ctctcgcttt gtggggacct actttgaggc cgatgggcaa gggtatgtcc aggttgacgg
    21601 gacggtgttc gcccagccga tttatgtggg ggacccaggt gcccatggcg tgcagcctga
    21661 tgacaaagtg gttatcgaaa tgctccgctt ccccaatcat tttcggcaag gtgagggagt
    21721 cattgtcgag gttcttgggc cgctagggaa acctggggtc gatacgctga caatcatgcg
    21781 ggaatttaat cttcccgagg agtttccgcc tgaagtccta gaagaagccc ggcaggttgc
    21841 ccaggccttc gaagagacaa ttcccccgga ccggtacgac gccacggcgg aaacaaccat
    21901 caccattgac ccagaagatg cccgcgactt cgacgatgcg gtgtcgcttg aacgcctggc
    21961 agatggtagt tggcggttag gggtccatat tgccgacgtg gcctacttcg tgccgcccgg
    22021 atccctgtta gatcacgagg cacgaaaccg cgggacgagc gtctatctcc cggatcgtgt
    22081 gatccccatg ttgccggaac taatatcaaa cggcttggcc agtttgcaac ccgggaaact
    22141 gcgactgacg aagtccgttt atatggagtt tacctcggaa gggatcaaga cagacgtcaa
    22201 aatcgagcga tcggtcatcc gcagcgatcg gcggctaacc tatgaacagg tggacgcctt
    22261 ctttgaggac ccggcggcct acaagcgggc gtggggaacg aaggtccacg atctcctctt
    22321 gcggatgcgg gagcttgccc gaatcttgcg ccaacgccga cgtgaccgcg gtgccttgga
    22381 attggtcatg ccagaggtca aggtcgattt ggaccgtcgc ggtcgggtcg ttggtgccca
    22441 tcttgtgcct aataccgaaa gccaccagat catcgaggag ttcatgctgg cggcgaacga
    22501 gagcgtggcg gagacgctcc gcgaccgcgg ttggttattc ctgcgacgga ttcatcgacc
    22561 acctaccgcc cgcaaattgc ggcaattgca agattttgtt gaggccctag cgtttccttc
    22621 ccgcgacctg gaggatcgct tcggtctgca atccctgctg gaaataacta agggtaagcc
    22681 ccaacaatat gcggtgcatt atgcggtttt gcggtccctt caacgggcca tctacagccc
    22741 gctggaggaa ggccactatg cccttgccag cgaatgttat tgtcacttca cgtcgcccat
    22801 ccggcgctat cccgacttga ccgtccatcg cctggtcgat gcgatgttgt tgaactttaa
    22861 gccgcg
//
LOCUS       DAOSUT010000062        21980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1539_length_21980_cov_9.682007, whole genome shotgun sequence.
ACCESSION   DAOSUT010000062 DAOSUT010000000
VERSION     DAOSUT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21980
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1539_length_21980_cov_9.682007"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1076)
                     /locus_tag="PLD05_07445"
     CDS             complement(252..1076)
                     /locus_tag="PLD05_07445"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOP77309.1"
                     /translation="MKRRICYLGDDDLSAAAAYLAGIMGHAGLPYAHVPSRRPVNGEI
                     LDEPYALYILSDYPSAGFSIEQMRRLAENVANGSGLLMIGGWESYFGRVGEYVNRPIA
                     DLLPVYLTTSDDRVNCPQGCYVRKARRHPITDGLPWQNPPMVAGYNRIVPKPDAQILL
                     VGERVWTVDTGKELQFSIVDTVPLLVIGKHGRGRVAALAFDVAPHWIGGMVDWGTRRI
                     VQQIPVGGFVEIGDDYAKFFRQLLAWTGRFEDMPDSYPEETTPREEVPLAPAPSKR"
     gene            complement(1205..1504)
                     /locus_tag="PLD05_07450"
     CDS             complement(1205..1504)
                     /locus_tag="PLD05_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOP77310.1"
                     /translation="MPTPRPTSLRMDGPDLVIEWNDGTVARYSPHRLRALCPCASCAE
                     LRSHSTDPPSPDRFSNVTFRQVTPVGNYAYQITFSDGHNTGIYTLDYLYRLRNLE"
     gene            complement(1885..3054)
                     /locus_tag="PLD05_07455"
     CDS             complement(1885..3054)
                     /locus_tag="PLD05_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP77311.1"
                     /translation="MKVRVNPFAKLLGFLWVFSVLALGGVTLAQDGHSNSPGTGPDPQ
                     IYQACVKKGIDFLINRGQLPDGSFSPEAGVGITGIAVFALLQHGLPVDHPAIRSGLGY
                     IERYIQPDGGIYSPDSFYKNYETCIAIMCLQAANKDGRYQDVIARAERFAKDIQWDES
                     EDISPSDYAYGGAGYGRHRRPDLSNTAFLIDALKSLGRGPDDPAIQKALIFVSRCQNL
                     ETEHNTTPFANKNPDGGFYYTWAAGGESMAGKTPTGGLRSYGSMTYAGLKSMIYAGVK
                     ADDPRVQAAKRWIAEHYDLESNPGLGQQGLYYYYHTFAKTMSALGEPYFVDASGKRHD
                     WRAELVAALVKRQREDGSWINMADRWMEGDPNLVTAYALLALACCRPENGSGTSQ"
     gene            complement(3307..4689)
                     /locus_tag="PLD05_07460"
     CDS             complement(3307..4689)
                     /locus_tag="PLD05_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP77312.1"
                     /translation="MSKATLLLVDDDRHLLNAMAVWLREQGYKVDTGASVQDARRELT
                     QRGYDVAIIDIRLPDGDGFEVLRFAKERYPEMPVILITGYGTVDLAVEAIRAGAFDFL
                     TKPLIDEELQLSIERALNQRRVIEENKNLKCQLDMRYGLDNIVGRDHRMIRIYDMIEA
                     VADTKATVLITGESGTGKSLIARAIHRRSGRRDKPFVEVACGALPETLLESELFGHVA
                     GSFTGATSDKIGKFKQADGGTIFLDEIGTASPALQVKLLRVLQDYQFEPVGGTQTITV
                     DVRVILATNEDLQKAVQMGRFRQDLYYRINVINIELPPLRERVTDIPLLAEHFLRRIC
                     EEMGRPVKTLSEEALAALQAYRWPGNVRELQNVLERAVLLGRHQTITLEDLPPEVAAA
                     LSPSYANADTAPLKQALQLPEKQIILQMLELHNWNRQETAKALGINRTTLYKKMRRLG
                     IQVPQGSTHT"
     gene            5131..5763
                     /locus_tag="PLD05_07465"
     CDS             5131..5763
                     /locus_tag="PLD05_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOP77313.1"
                     /translation="MAIRVVIADDHEVIRWGLKEMLRGTEIEVIGEASTGVEAVEKVE
                     NLRPDVVVLDIRFPDLDGLETLAMMREHGLDTRVVLLSSYDYQSYAARAVALGASEYL
                     LKGASRDEILRAIRAAAASQPPAPDSRLGVVAEAMQSKTTVEDDAVQLTQRETQVLRH
                     IALGLSNREIANSLQISVETVKEHVQNILRKLGVSDRTQAAVWAVRKGLV"
     gene            5914..6552
                     /locus_tag="PLD05_07470"
     CDS             5914..6552
                     /locus_tag="PLD05_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77314.1"
                     /translation="MRMSVAIISQVVPATLVEDLAGRVASFWPVVAVVLVIVLVWVVR
                     AVFFRRRPPLPPEPDLRIDLTSLHPEMPLPSPRLEFFGLPVRLVVLVLAPAGRDGYLP
                     PPEQWNDLFESIVPGLAEVVRTHQPIVVRWPPQLSAQGFIHKYFQNVHLPGDRGRGTP
                     WCSAAGVARYSGRAFLVGMTFYAARANHYSQETINSEGDWYRLLRVTLADGQ"
     gene            complement(6871..8196)
                     /locus_tag="PLD05_07475"
     CDS             complement(6871..8196)
                     /locus_tag="PLD05_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOP77315.1"
                     /translation="MNHSSEGNYVWKECLEYAAKTDIGLRRSNNQDAHAEDLAGSLEA
                     FRARGHLFIVADGMGAHAAGETASKKAVEAIPLMYRKLKDLPVKDALREAVLEANRQI
                     FECAQANDNFRGMGTTISALVMSSEGAFVAQVGDSRVYRCRKHSIEQLSRDHSLVWEL
                     RSLPEEIRPQYIPKNIITRSLGPQTDVEVDVEGPFPIEADDRYVLCTDGLSGQLSDEE
                     IGQITTCLPPDEATQALVDLANLRGGPDNITVTVVHVKSAPLCRLSQGNGQSVVSRKA
                     HPPRWLLLAFVVSLLVVLVVGIAGAMSGRAWQEWAAAFVTAILCAGLLLALLISRKLR
                     RISQSLVAPTKQGAPYATADCCPRRQTLENLLQILAEIEDLARREGWDADWSRLEELK
                     HIAVGSPDNELASGIAAACRAISLLMSVARAQRGSRSNHSTGPGFYRRF"
     gene            9194..11566
                     /locus_tag="PLD05_07480"
     CDS             9194..11566
                     /locus_tag="PLD05_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOP77316.1"
                     /translation="MSALIFHNVRASLLVLLFLAPFAAGADSGPIRLPPVDQDTAGLP
                     GENPFTAQGLEMRIARRGSDEVPSPLPLDVIGPNVRSTRIEQAARQADLLTFKGYELA
                     GRGAFFAARAQFRAALFVLAQALDTEHRTSEHSQMLDAAWVALKEAEDFLPRPGKQVT
                     SASVPGIIARHDSSVLKDHTFGVLTPLEAYQIYLNFAQDLFSRAVAAEITGSMALRGL
                     GKIYAETSAANGLLQGDSEAQAVVFYQAALNVWPENYMAANDLGVLLARGGRWDDAIS
                     VLEYARRIRNDPVIVRNLVKVYRQQGLADRAAKLLAEAGSLTRETEATDADVVRSVAW
                     VPPEVLDQADRTLAKGPVGESRFSSSNPATPSVSRPTVPIKSTTGRLGLLPLLTMGPA
                     DSVAGQSGAARGQNGVVNTPTEFVPGSSAETQERPVSLSPTGDVVTTGVSLPNPASPC
                     PRCAVDGSVCDCSKWGGWQRARMIAWEKYAQGEYVGPARLAHVPEYRLRVDDELQIVY
                     RITREETSRPYRLNVGDQLRIESATDPTLNRDVLIQPDGTVTLLLLGQVKATGLTVTQ
                     LRDRLEQLYRRYYRAPAITVTPILVNSKLEDLRATVDRRAGEGGQSQNVRITPEGTIA
                     LPGLGSVKAQGLTLSELQAELNERYREEVEGIEAMPILVQRAPRYVYVLGEVESPGRF
                     ELTGPTTVIQAISMAGGWKIGANLRQVVIFRRGDDWRLLATMLDLQAALHGNKATPCD
                     EIWLSDSDVVIVPKSFLQRSDDFIEMVFTRGIYGVLPLQTTINFSKLSTI"
     gene            11812..12177
                     /locus_tag="PLD05_07485"
     CDS             11812..12177
                     /locus_tag="PLD05_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77317.1"
                     /translation="MSARVLVADDSSTMRKIIIRSLQAVGVQDAVEAADGNEALALFQ
                     PGAFNLVLTDWNMPGKSGLEVVQGIRAQDPNVPIIMVTTEAEKSRVLQAIQAGVSDYL
                     VKPFTADTLREKLEKHGCG"
     gene            12710..13630
                     /locus_tag="PLD05_07490"
     CDS             12710..13630
                     /locus_tag="PLD05_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77318.1"
                     /translation="MGMHRRHFLVSTAALAAGGMAFSGAKMVWAVPGEISTPRSGAQL
                     KLSSQLGVIPGKSLEEKLEKMKKWGFDAVELPGDIVGKVEMYAKAVANAGLKISAVCW
                     GSAGGALVSEDENRRKDGIRMIKEVLSAAGELQSTGVIYVPAFNGQTKLTNQEIRKVL
                     LDILPEIGEHAVSVKSRVLLEPLNRKEAFFLRQVADAAAICRDVNSPGICLMGDFYHM
                     YIEEPSDFAAFVAGGPWLHHVHLASRIRVLPGQDERSFVDGFRGLKWVGYQDYCSFEC
                     GVQGDREVEIPKAMAFLHEEWEKATLPGVA"
     gene            13735..14730
                     /locus_tag="PLD05_07495"
     CDS             13735..14730
                     /locus_tag="PLD05_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP77319.1"
                     /translation="MNKQLCVMVLVALSLGTSFLARAEEGRARDEIIDPAVAAQDPDF
                     AIQGEYLGEGVLPDGTQGKLGVQVVARGGGQFEAFILVGGLPGEGWDRSRPRIAGKAV
                     REDGKASVQAEGWQGVIENGEMTLKHEKGEVKLKRVERKSPTLGQKPPEGAVVLFDGR
                     NADAFDGGTVLPDGSLLSGVTTKQGFGSYTLHLEFRLSWMPRALGQARSNSGVYLHDC
                     YEVQVLDSFGLTGEDNECGGIYRVKAPDLNMCLPPLVWQTYDIEFTAPKYENGQKVAN
                     ARLTVRHNGVVIHDNLELPGATPGRQQEGPAPRPIHLQAHGNRVTYRNIWLVPKD"
     gene            15590..16777
                     /locus_tag="PLD05_07500"
     CDS             15590..16777
                     /locus_tag="PLD05_07500"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP77320.1"
                     /translation="MHLGRVIGRLLSAWHLGLAYGVLAVALLPGAALRGGEAAQPVVE
                     ASVVEQVDVFISGEKGYHTYRIPSLLRSPDGSLLAFCEGRKHGLGDSGDIDLVLRRLA
                     PGQSTFGDLLVVWDDGPNTCGNPCPVVDQQTGVIWLWMTHNLGEDHERDIVARKSRGT
                     RTVWLAKSVDHGQTWTQPVNMTEHVKLPEWSWYATGPGCGIQTRSGRLVIPCDHIADD
                     GTWGSHVILSDDHGQTWRLGGVAKPKTNECEVVELSDGRLLLNMRNYNRQFPCRAVAE
                     SSDGGITWSEVRYDQQLVEPVCQASIRRVDGITIGGRPLIAFSNPADTKERRRMTVRF
                     SPDDGQTWPWSVVVWEGPAAYSCLASLPDGTVLLLYERGNKHAYERITLARIVIRVKS
                     PGS"
     gene            16870..18318
                     /locus_tag="PLD05_07505"
     CDS             16870..18318
                     /locus_tag="PLD05_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOP77321.1"
                     /translation="MLQRWFQLREHGTNVPREFLAGLTTFLTMAYIIVVQPQVLSGKM
                     FGNETGLDFGAVTTATCVSAAIATLIMGIYARYPIALAPGMGENFVFVLSLIPAAQVW
                     LEQEIQAGRIQAGTTTAWSIALGVIFYSGVLFLVLSLLGIRERLLEAVSPSMRNAIAA
                     GIGLFIALLGLQGASVIVGAPGQIVKLSPRLTSPDVIVFFVGLMVTAAFYARRIPGAI
                     VIGIFTATLLAVGLRQVIPLLPGLAETEIVRQSQLMTRFAPAEHLFSGPPSMAPTFAK
                     MDLIHALALPMIPYILIFLFMDVFDTLGTLVGVSEQAGFIRDNRIPRAKQALSADAVG
                     TVVGAVCGTSTVTSFIESAAGIEQGGRTGLTAVIVAILFLVAPCCYPLIEMVGSYPPI
                     TAAALLIVGSMMMKNVLKIEWDQATEAIPAFVTMTGIVFSYSIADGLALGFIVYPLIK
                     FLAGKGREVPWLMYGLAVVLLAYLVFAKAHMG"
     gene            complement(18528..18806)
                     /locus_tag="PLD05_07510"
     CDS             complement(18528..18806)
                     /locus_tag="PLD05_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOP77322.1"
                     /translation="MATFVMLGKYSPEALRQISAERTEKFVKTIAELGGSVRAMFATL
                     GEVDLVLIVDLPGIEQAAKASVVVSKDTGIHFSTHPAIAVKDFDQLVG"
     gene            complement(18895..20337)
                     /locus_tag="PLD05_07515"
     CDS             complement(18895..20337)
                     /locus_tag="PLD05_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOP77323.1"
                     /translation="MGKTWEYKDLKALLEPFQQTHLLAGWEDLSEEGRARLAAQIREL
                     DLPLIARLFRDRNLPIDIASLVERAVSPRGVRLDSEGAGLSPADAYRLGQGVLQRGEV
                     AALLVAGGQGTRLGFPHPKGMFPIGPISGHSLFQIHVEKVLARSRKAGCPIPLAVMTS
                     PATHQETVEFFSRHGRFGLPEEDLYFFCQGTMPSVDAATGKILLSAPGDLALSPDGHG
                     GMLAAIIRSGTLEKLASRGIKYLFYFQVDNPLVDILSPSAIGYHIYFGSEMSTEVVAK
                     ETPFDRVGNVIEVDGRLYVVEYSDLPDELAQKRSPDGRLLLWAGSIGVHIINVDFLFR
                     MYEQVDALPYHLAYKKVPYYDPASGPVTPTKPNAIKFERFIFDLIPHAKNAIVIEVNA
                     AQHFAPLKNAPGADRDTPEHVRQQMIALHRSWLERNQVAVCDGVAVEISPLFAVEPDD
                     LKGRFPPGYRITEPTFFSHTTWVSSAESRR"
     assembly_gap    20385..20484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20661..21728
                     /locus_tag="PLD05_07520"
     CDS             20661..21728
                     /locus_tag="PLD05_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HOP77324.1"
                     /translation="MVLTFLRFALLIVLAGVAVIVVKSGLYPQDHQWMAWVLVAVIMA
                     IGGLAILVDVLIPRKPLEIITSVYFGLIVGLFLSYVAGLALTPVFESLQVDEQVRNVV
                     QLIVSAILCYTCISFLFQTKDDFRFIIPYVEFTKEVRGRRPYILDTSVIIDGRIADLV
                     ETNLFDSRLIMPRFVLTELQAIADSADRTKRARGRRGLDILNRLRNNPDVDFVIWDRD
                     LPEFSGQSVDLKLITLAKHLEGRLVTNDYNLNKVARVHGVRVININDLANALKPRFLP
                     GEQIQVRLVRPGEEEQQGVGYLDDGTMVVVEDGREYVGRTVVLNVTSVLQTSAGRMVF
                     GRYDRTVEHNHEAREDRVARS"
BASE COUNT         4539 a         6021 c         6471 g         4849 t
ORIGIN      
        1 ggtcgtttcc aattcgccct gacggggggc actcataatg ttccggggca ggcacagggg
       61 cctgccccta caggtttcaa tttcgcccgg agcttcaggt caatcttccg cgcagcagca
      121 tcgctcgcat acgaatgcac gcaggtgcca cttgttgccc ggaggttccg gccaccagag
      181 agcgtgatct ttgccgcggt ctagtgcata acacgcatgc atcgctacgt catttaagac
      241 cacgcggcga gctagcgctt tgacggtgcc ggtgccaacg gaacttcttc ccgaggggta
      301 gtttcttcgg gatagctgtc gggcatgtct tcaaagcggc ccgtccaagc caacaactgt
      361 cggaagaact tcgcgtaatc atcgccaatt tccacgaaac caccgaccgg gatttgttgc
      421 acgatacgac gtgtccccca atcgaccatg ccaccaatcc aatgaggggc gacgtcaaac
      481 gccagggcgg ccaccctgcc ccgcccgtgc ttgccgatga cgaggagcgg aaccgtatcg
      541 acaatggaga actggagttc cttaccagtg tcaacggtcc aaacacgctc accaaccaag
      601 aggatttgcg catccggttt aggcacgatg cgattataac ctgcgaccat cgggggattt
      661 tgccagggta gcccatctgt aatagggtga cgacgcgcct tgcggacata gcagccttgt
      721 ggacagttga cccggtcatc ggaggtcgtc aaatagacgg gcaacaggtc ggcgatgggg
      781 cgattgacgt attcacccac tcgtccgaag taactctccc agcctccgat catcaaaagg
      841 cctgacccgt tggctacgtt ttctgccaac cgtcgcattt gctcgatgga aaaaccggca
      901 gaagggtaat cgctcaggat ataaagtgcg tagggttcat cgagaatctc cccattcact
      961 gggcggcgac tggggacatg agcatagggc aagcccgcgt gtcccataat cccggccagg
     1021 taggccgctg cagccgaaag atcgtcatca cccaaatagc aaattcgccg cttcatagaa
     1081 tattgctcgc tattcagaaa aaggaaaagg gcgaaactct caaggtttgt gttttattgt
     1141 gaccagcggt ggaccttccg acaagtcaaa tccatgggcc accgtgtacc cagccggcat
     1201 ggatttactc gaggttgcgg aggcgataaa ggtaatcgag cgtgtagatg ccggtattgt
     1261 ggccgtcgct aaaagtgatc tgataagcgt aattgccgac tggggtcacc tgtcgaaaag
     1321 tcacgttcga gaaacgatcc ggcgagggag ggtcggttga gtggctgcgc aattcggcgc
     1381 aactggcgca gggacacaag gcccgcaacc gatgcgggct gtaccgggcc acagtcccgt
     1441 cgttccattc aatgaccagg tcagggccat ccatccgcag actggttggt cgcggcgtcg
     1501 gcatgtgggt tggcctccag aaggtgccta acgccctgag gtttctagcg agcgaacccc
     1561 agaggcgatc gcttcgtcga tcatggtcac agcgttcttc gtcttggggt aattaaaaac
     1621 gaggcaggct tttcttgcaa tggtgccgcg gcagggtctt cacacgaggc tgagagttct
     1681 gcatcttggt gaataaccca agccccagtg agcagccagt ccttagagcc catcccacaa
     1741 cctcccctcg cccgttcggt gggagagggg ctggcggtca gggtggctgg aatcgctggt
     1801 ggttttccga tcaactccga cgttcgcttg ccctcacccc aaccctctcc cggagggaga
     1861 gggggacctt tttgggatag gcccttattg tgacgtaccc gatccgtttt ccggtcggca
     1921 acaggctagg gccaaaaggg cgtaagccgt caccagattg ggatcaccct ccatccagcg
     1981 gtcggccata ttgatccaag aaccgtcttc gcgctggcgt ttgaccaagg ccgcgacgag
     2041 ttccgcgcgc cagtcgtgtc gcttgccgga ggcatcgaca aagtagggtt cccctagggc
     2101 gctcatcgtc ttggcgaagg tgtgatagta gtagtagagg ccttgctggc ccaagccagg
     2161 gttggactcg aggtcataat gctcggcgat ccaacgtttg gcggcttgaa cgcgggggtc
     2221 atcggccttg acgccggcat agatcatact tttcagacct gcataggtca tcgaaccata
     2281 actgcggaga ccacccgtgg gggtctttcc cgccatactt tcgcccccgg cagcccatgt
     2341 gtagtaaaac ccgccgtcgg gatttttatt ggcgaagggg gtggtgttgt gttcagtctc
     2401 gagattttgg caccgcgaaa cgaagatcaa ggccttttgg atcgccggat cgtctggtcc
     2461 tcgaccgagt gacttgaggg catcgatcaa aaaggccgtg ttggagaggt cgggacgccg
     2521 atgacgacca tatccggcac cgccgtaagc gtaatcactg ggagaaatgt cctcgctttc
     2581 gtcccattgg atgtccttgg caaagcgctc ggcccgggcg atcacatctt ggtagcgacc
     2641 atccttgttg gccgcttgaa gacacataat ggcgatacac gtttcgtagt ttttgtagaa
     2701 gctgtcgggg gagtagattc caccgtcggg ctggatgtac cgttcgatgt agcctaggcc
     2761 agaacgaatg gccggatgat ccaccggcaa tccgtgctga aggagggcaa acacggcgat
     2821 ccccgtgatc cccactccgg cctccgggga gaacgaaccg tcgggcaatt gtccgcggtt
     2881 gataaggaaa tcaatgcctt ttttgacaca ggcctggtaa atctgcggat cggggccagt
     2941 gccgggggaa ttactatgtc cgtcctgcgc caacgtgaca ccgcctagtg ccaataccga
     3001 aaagacccaa agaaaaccaa gcaatttagc aaacgggtta accctaactt tcatagcaca
     3061 aactcctggg caaaaaatcc tgcgcgagct gactgccgtc gagtaagcga gacgaacagg
     3121 tcaatcaaag caaagtccgt gccgagacac agtccctttt ccagcggtgg gaaaatatgt
     3181 gggattgttt gggcggtttc caacatcata gtcgcgggaa ggcggccgcg gcaagcgcga
     3241 cagtcgtttg gcgcaacatg gtgtgtcgaa aaatgcgaca tgattagggc atcgccttca
     3301 gtaccatcag gtgtgagtag aaccttgcgg cacctggata ccgagtcgcc tcattttctt
     3361 gtagagcgtc gtgcggttga tgccgagggc cttggcggtc tcctgtcgat tccagttgtg
     3421 aagctccagc atttgcaaaa tgatttgctt ttcgggcaac tggagtgcct gcttaagcgg
     3481 tgcggtgtcg gcgttggcat aacttggcga aagagccgcc gctacctcag gcggaaggtc
     3541 ttccagtgtg atggtctgat gtcttcccaa taggacggcc ctttccaaca cgttttgcag
     3601 ttcgcggacg ttgcccggcc agcggtaggc ttggagggca gccagggcct cctcgctgag
     3661 cgttttcacg gggcgtccca tctcttcgca aatacggcga aggaagtgtt cggccaaaag
     3721 tgggatgtcg gtgacccgtt ctcgcaacgg tggcaattcg atattgatca cgttgatgcg
     3781 gtaataaagg tcttggcgaa atcgccccat ttgaacggcc ttttggaggt cctcgttggt
     3841 cgccaggatc acccgcacat ctacggtaat cgtttgcgtg ccaccgaccg gctcgaattg
     3901 atagtcttgc aagacccgca agagcttgac ctgcaaggcg ggactggccg tgccgatctc
     3961 gtcgaggaaa atcgtgccgc cgtcggcctg cttgaatttg ccgatcttgt ccgacgtcgc
     4021 acctgtaaac gacccggcca catgtccgaa gagttcactt tctaaaagcg tttcggggag
     4081 ggcaccgcag gccacttcga cgaaaggctt gtctcgccga ccgctccgcc ggtggatggc
     4141 gcgggcaatc agggacttac cagtgccgct ttcccccgtg atcaagacag tggccttagt
     4201 atccgccaca gcctcgatca tatcgtagat gcggatcatg cgatggtcgc gacccacgat
     4261 gttatcgaga ccatagcgca tgtctagttg gcacttgaga tttttgtttt cttcgatgac
     4321 gcggcgctga tttaatgccc gctcgatgga cagttgcaat tcctcgtcga tgagcggctt
     4381 tgttaggaag tcaaaggccc ccgcccgaat ggcctccacc gccagatcca ccgtgccgta
     4441 ccctgtgatg aggatgactg gcatctcggg ataacgttcc ttggcaaaac ggaggacctc
     4501 aaacccgtcg ccatccggca aacggatgtc aataatggcc acgtcgtagc cacgctgggt
     4561 cagttcgcgg cgggcgtcct ggacactggc ccccgtgtca accttgtagc cttgttcgcg
     4621 gagccagacg gccatcgcat ttaggagatg ccggtcatcg tccactaaaa gcaacgtggc
     4681 cttgctcatc ttggtgacct ttgttgctca aaaccaactt tccgattggc gaggtgctgc
     4741 ggaaaagagt tgctccctcc cttgcctacc gtaaaaaccc cgttggtaac gcgatcccgc
     4801 ccatggcaag ggaggccgtc gaaaagtatc tacgtcagaa acggcggctt gtcaacatga
     4861 gaaaaatagg aaacatggga ggtcattttg aggcaaagta accattttag ggggaaagct
     4921 tataaccaga cttggtagtg gtttctttcc caccgaaaag aagtactatt aataaggcgg
     4981 tccacccctt gtgggtatga tcgaccatct tgacgcaggg accaagtggg catactcacc
     5041 cgttgaggtg gcaacccttt ggaagccagt cgattgcgtc gatttggggt catcggggtg
     5101 aacagttaga gacatgagga ggcctttacc gtggcaattc gcgtggttat cgctgacgat
     5161 catgaggtga tccgttgggg attgaaggaa atgttgcggg gaacggaaat cgaggtcatc
     5221 ggggaggcct cgaccggtgt tgaagcggtg gaaaaggtcg aaaatctacg ccctgacgtc
     5281 gtcgtgctgg acattcgttt tccagatttg gacggtcttg agacgttagc catgatgcgg
     5341 gagcacggcc tcgatacccg ggtggttctc ttgtcctcct acgactatca aagctatgcc
     5401 gcccgtgctg tagcccttgg ggcgagtgag tatctcctta aaggggcctc gagagacgag
     5461 atcctgcggg cgattcgcgc tgcggcggca agccagccgc cggctcccga ctcgcggctg
     5521 ggtgtggtgg ccgaggccat gcagtccaaa acaaccgtcg aggatgacgc cgttcagttg
     5581 acgcagcgag aaacgcaggt cctgcggcac attgccctag ggctttccaa tcgcgaaatc
     5641 gccaattcgc tccaaatcag tgtggaaacc gtcaaagagc atgtccagaa cattctgcga
     5701 aaactaggcg tcagcgatcg cactcaggcc gcggtttggg cggtgcgaaa gggcctggta
     5761 tgagcttttg gccaatgtct tcctggggtt tggttgggga cgcacagtgc gccaaagctt
     5821 aagaagaatt aaaaggggaa cgccattgcg aaaaagagct tcctcatggt tggatccccg
     5881 gggctttgcg gcctgttttg agcaggacta aggatgagga tgtcggtggc gataatctcg
     5941 caggttgttc cagccacact tgtggaggat ttggcggggc gggtggcgtc gttttggccg
     6001 gtcgtcgcag tagttctggt gatcgtcctg gtttgggtcg tgcgggcggt gtttttccgc
     6061 aggagaccgc cgctaccccc ggaaccggac ctccggattg acctcacatc gctccatccc
     6121 gaaatgccgc tgccatcccc gcggcttgag ttctttgggc tccctgtgcg cttggtggtt
     6181 ttggtgctcg cgcctgccgg aagggacggc tatttgccgc ctccggagca gtggaacgac
     6241 ctttttgagt ccatcgtccc cggcctagcg gaggttgtcc gcacccatca gccaatagtg
     6301 gtaagatggc caccccaact gagtgcccag gggtttatcc acaagtactt tcaaaatgtt
     6361 cacctgccgg gtgatcgggg acgtggaacg ccctggtgtt ccgcggccgg agtggcgcgg
     6421 tattctggcc gggcattcct cgtggggatg accttttacg cggcccgggc caatcactat
     6481 tcgcaagaga ccatcaacag cgaaggcgat tggtatcgtt tgcttcgcgt gacactggct
     6541 gatggacagt gagaaaggcg ctgtcgattg atacgccaat gttagtcgcg gctattgatt
     6601 gcagccacca tggcgactgg acccgcactc gcgaggtcat tcatttgagg cctattccaa
     6661 aattccctct ccctctggga gagggttggg gtgagggcaa gcgactgtcg gagtggatca
     6721 gaaaacccgc agcgattcga gcgaccctca cccccgaccc ctctcccgcc gaacgggcga
     6781 agggaggttg tgggataggc tcttaactgg gataaccctt ctagttgccc gcccttagtt
     6841 ccacgaatgg gcaagcacga aggccgggtg ctaaaacctt cggtaaaaac ctgggcccgt
     6901 ggaatggttg ctccgagaac cccgctgggc ccgcgccaca ctcatgagca gcgatatcgc
     6961 ccgacatgca gcagcgatcc cggaagccaa ttcattgtct ggggatccca cggcgatatg
     7021 tttcaactct tcgagtcttg accagtcggc gtcccagcct tcgcggcggg caaggtcttc
     7081 gatctctgcc agaatctgca agaggttttc caatgtttgc cgtcgggggc aacagtccgc
     7141 tgtggcatac ggtgccccct gcttggtcgg cgccacgagg gactgagata tgcgtcgtaa
     7201 tttccgggaa atcaacaagg ccaagagaag ccccgcacag aggatagccg tcacgaaagc
     7261 ggctgcccat tcctgccacg cacgtccgct catggctccg gcgatcccca ccacgagcac
     7321 gacgagcaac gacacgacaa aggcaagtag gagccagcgc ggcggatggg ccttcctcga
     7381 gaccaccgat tgcccgtttc cttggcttag ccgacataat ggagcagact tgacgtgaac
     7441 caccgtgacc gtaatgttat ccggcccacc gcggaggttg gccagatcca ctaaggcttg
     7501 tgtggcctcg tcgggtggaa ggcacgtagt gatctgtccg atctcctcgt cgctcaattg
     7561 cccggatagg ccatcggtac acagcacata acggtcgtcg gcctcgatgg gaaaggggcc
     7621 ttccacatcc acctcaacgt ccgtctgcgg gccaagggaa cgcgtgatga tgtttttcgg
     7681 gatatactga ggtcgaattt cttcgggcag actgcgaagc tcccagacga gactatggtc
     7741 tcgggagagt tgctcaatgg agtgcttgcg gcagcgatag acccggctgt ctcccacttg
     7801 ggccacgaag gcaccctcgg aagacatgac aagcgcgctg atcgtcgtgc ccatcccgcg
     7861 aaagttgtcg ttggcttggg cacactcaaa gatctgccga ttggcttcta agacagcctc
     7921 tcgcagggcg tccttgacgg gcaagtcctt caacttgcgg tacatgagcg ggatggcttc
     7981 gaccgctttc ttgctggccg tttctcccgc tgcgtgagca cccatgccgt ccgcaacgat
     8041 gaacaggtgt ccacgagcgc gaaatgcctc caggctaccg gcgaggtcct cggcatgggc
     8101 gtcttggttg ttcgaccgcc gcagcccgat gtcggtcttg gctgcgtatt cgaggcactc
     8161 cttccaaacg taattgccct cagatgaatg attcatcaca ttggccgttc aaggcatgga
     8221 aaacgttcat tcatttgacc acccgaattg cggcattgtc aatatttttg gcccccggat
     8281 cgtacgcttg ccgataaccg tgccatcgcg tccgattgtc atttaaattg ccaccgcaaa
     8341 ctggccagtt gaaatctgtg tcagaaaaca ccctcctatc ttgcagtggg ggcccgccat
     8401 ctatgccacg gccacaacac cgcgtcattg cccaccgccg ggtttggacg gactggccat
     8461 gttccccaaa atctacccgg ttgcgcgcca tcgtgtccgc ggcgctgccg attgatgcgc
     8521 cattgtgcgc ccctgtccaa gccgggaatg cctagatcca gttgtgccgc ctcaaagacc
     8581 ttcgtgctag gcccgaaccg ccacgaccgc ccgacgcttt taggaaatcg aggaccgccg
     8641 tcgtaacctt gactctcctt tggctgggtt cagccagacc tactgcacag cccaaacggg
     8701 taacgtctcc cgcaacatcc gacacacatt gagattatcg gacgccacgc tttctgtatc
     8761 ttgttcaata tccgggattt ggttattttg cccaatctta ctgatcctca ttttagtccg
     8821 cagctatcgg caggccaacc tttaccggcg gcagcaggca agaaaactgc tggccccatt
     8881 tctctttcgc agcactaaag ttgtcggacg ttggtaagct ctgccgcgtt tcatgaacgt
     8941 gccgagacac aagcagcttt gatatcgact cggcgcccgg ttgtgtcgag cttgtcgggt
     9001 attacgaaat ccaggtcttt gaccgttctg tcgagaaaaa cgcgctcatc gctttggtcg
     9061 gagatgctcg ctctcgcgga cgatgggatt gtaagaacag attgtcagga gccggttgtg
     9121 atcgatttat gtgatgcgcc gcgcagtagc aagaaagaac tcaacgatga tactccaggc
     9181 agcggtttca aagatgtccg ctctcatctt tcataacgtg cgggcaagtc ttttggttct
     9241 gcttttttta gccccgtttg ctgccggtgc cgattccggt cctattcgac tgccccccgt
     9301 tgaccaagat actgccggct tgccgggcga gaatcctttc acggctcaag ggcttgagat
     9361 gcggattgcc cgccgcgggt cagatgaggt accctcgccg cttccgcttg acgtaattgg
     9421 accgaacgtc cgctccacac gaattgagca ggccgcccga caggccgatt tgttgacgtt
     9481 caaaggatac gaactagctg gacgaggggc cttttttgcc gctcgggccc agtttcgcgc
     9541 tgccctcttc gtccttgccc aggccctcga taccgaacac cggacgtcgg agcactccca
     9601 gatgctggat gccgcctggg ttgccctaaa ggaggctgag gacttcctcc cgcggccagg
     9661 aaagcaggtg acatccgcat ccgttccggg gatcattgcc cgacatgatt cctctgtgct
     9721 taaagaccat accttcgggg tgctgacccc cctggaggcg tatcagatct atctgaactt
     9781 tgctcaggac ttgttttctc gcgcggtagc cgcggaaatc accggctcga tggcccttcg
     9841 cgggttgggc aaaatctatg cggagacgag cgcggcaaac ggtctgcttc agggtgattc
     9901 cgaggcccaa gcggtggtct tttaccaggc agctctcaat gtctggccgg agaactacat
     9961 ggctgccaac gaccttggcg tcctgttggc ccggggtggc cgatgggacg acgccatttc
    10021 cgtattggag tatgcccgtc ggattcggaa cgatcctgtc attgtgcgca atctagtaaa
    10081 agtttatcga caacaaggac tggccgaccg cgccgcgaag ctacttgcgg aggctggttc
    10141 tctgacgcga gaaaccgagg cgaccgatgc cgacgttgtc cggagtgtgg cttgggtccc
    10201 gcccgaggtg cttgatcagg cggatcgtac cttagcgaag ggtcctgttg gcgagtcgcg
    10261 gttctcatct tccaaccctg cgacgccgag cgtatcccgc ccaacggttc ccataaaaag
    10321 caccacgggc cgattgggct tgttaccatt gttgaccatg ggccccgccg actctgtcgc
    10381 cggccaatcc ggcgcagctc ggggccagaa tggagtcgtc aataccccga ccgagtttgt
    10441 gccgggttct tcggcggaaa cacaagagag gccagtttcc cttagcccga caggggatgt
    10501 tgtaaccacg ggtgtctcgt tgccgaatcc cgcttcgccg tgtcctcggt gtgcggtgga
    10561 tggttcagtc tgtgactgct ccaagtgggg aggctggcag cgggcacgga tgatcgcttg
    10621 ggagaaatac gcccagggag aatacgtggg ccccgcgcgg cttgcccatg tcccggaata
    10681 ccggctccgc gtggacgacg aactgcaaat cgtctatcgc atcacgcggg aggagacctc
    10741 gcgaccgtac cgcttgaacg tgggagatca actgcgcatc gagtcagcca ctgatcccac
    10801 attgaaccgc gacgtcctca tccaaccgga tgggacggtg acgctccttc ttttgggtca
    10861 ggtcaaggcc accggactta cggtaacaca actgcgagat cgcctcgagc aactttatcg
    10921 ccgctattat cgtgccccgg ccattacggt gacgcctatc cttgttaaca gtaagctgga
    10981 ggacctgcgg gcgactgtcg atcgccgtgc tggtgagggt ggccaaagcc aaaatgtccg
    11041 tatcacgccg gagggaacaa tcgcgctccc cggtttgggc tcggtgaagg cccaagggct
    11101 gacgttgagc gaacttcagg ccgaattgaa cgaacgctac cgggaagaag tcgaggggat
    11161 tgaagccatg ccgatcctgg tgcagcgggc cccgcgatac gtttacgttt tgggagaagt
    11221 ggagtcccct ggccggtttg agttgaccgg cccaaccacg gtcatccagg ccatcagtat
    11281 ggccggcgga tggaaaatag gggccaatct gcggcaagtg gtgatcttcc gccgcgggga
    11341 tgactggcga ttgttggcta cgatgctcga cctccaggcg gccctgcacg gcaataaggc
    11401 aaccccgtgc gatgaaatct ggctgtctga ttcggatgtg gtcattgtcc ccaaaagctt
    11461 cctccaacgg agcgacgatt tcatcgagat ggtgttcacc cgcgggattt acggcgtcct
    11521 accgctgcaa acgacgatta acttctcaaa gctcagcacg atttgagcgg ataccaccca
    11581 cgggggcgtt cttcggtcgg tttgtcgttt tcttgacagg cgcagttgcg tgacctacgt
    11641 ttttacaaac cgaaccgccg tgcacgtctt gtcagaggcg gttcacgtgg agtaagcgcg
    11701 ccggggattc ctagcccaaa ttggccaaat agggaattcc cacttttcgg gaaacttgtc
    11761 ataccatctt cgatcgaacg catcttttga caatgacaca agggaggagc gatgagcgcc
    11821 cgagttcttg ttgccgatga ctctagcacc atgcgcaaaa ttatcattcg ctcgcttcag
    11881 gccgtgggcg tgcaggatgc cgtggaggcc gccgacggaa acgaggcctt ggccttgttc
    11941 cagccagggg cttttaacct cgtgttgacg gattggaata tgccggggaa atccgggctg
    12001 gaagtggtcc aagggattcg cgcccaagat ccgaatgtcc ccattatcat ggtgaccaca
    12061 gaggccgaga aaagccgcgt cttacaggcc attcaggcgg gtgtttccga ctatctcgtc
    12121 aagcccttca cggcggatac cctccgtgag aagctggaaa agcacggttg cgggtaatgg
    12181 ccgggccgga cttgatcact ggagtctgtg gaaaagcgga acgtcgagct tgggtttctc
    12241 gcctatcggt tcggcggtct tgggcgtcag gagccattgt ccgcaaaaaa atacagcccg
    12301 gcagatagct tgacctatcg ccgggctgtt cccccctggg cacattacgt gtggtccgag
    12361 tttggcctga tccattccgg tcttatgtcc gcatcccgcg ctcgcgtcca agaccgactt
    12421 gcactgtgac ccggacagtt ataggtatcg accggcacaa tccctaatct tgagaaactc
    12481 tgtaaactcg ttacagccgg tacagcacgt tccaacggac ccaggagata gtggctactc
    12541 cgttgctgat gctggagata ttctaagcgg taccccctac cgggcaatgt ccgcatagtt
    12601 gacacggccg gggaacgggg ctgaaaatca aagcttgagt cccctgtggc tttcaagcaa
    12661 gcattcggtc cttcatgtag cccttttagc aggagaggag gttctatcca tgggtatgca
    12721 ccgtcgccac ttcctggttt cgacagccgc cttggccgcc ggtggtatgg cgttttccgg
    12781 cgcgaagatg gtatgggcgg ttcccggcga aatctcaacc ccacgatcgg gtgcgcagct
    12841 caaactgtcc agtcagttgg gcgtcattcc aggaaagagc ctagaagaaa aactagagaa
    12901 aatgaagaag tggggcttcg atgctgtgga gttgcccggt gacatcgtcg gcaaagtgga
    12961 gatgtatgcg aaggctgtgg ccaacgccgg gttgaagatc agcgccgttt gctggggctc
    13021 cgccggtggt gccttggtct ctgaggacga gaatcgccgg aaggacggta tccgcatgat
    13081 caaggaggtg ctcagcgctg cgggcgaact gcaatcgacg ggggtcattt atgtcccggc
    13141 attcaacggg caaaccaagt tgaccaacca ggagattcgc aaggtcttgc tggacatcct
    13201 cccagagatc ggtgaacacg ctgtgagcgt gaaatcccgc gtccttctgg aacccctcaa
    13261 ccgcaaggag gcattcttcc tccgtcaggt ggcggatgcg gcggccattt gccgggatgt
    13321 taatagtccg gggatttgtc tgatgggcga cttctatcac atgtacatcg aggagccgag
    13381 cgacttcgcc gcctttgtgg ccggtggacc gtggctccat catgtccatc tggcttcgcg
    13441 gattcgagtc ctacccggac aggacgaacg ctcatttgtc gatggttttc ggggcctcaa
    13501 atgggtcggc tatcaggatt actgtagctt tgaatgcggt gtccagggtg accgcgaggt
    13561 ggaaatcccc aaggccatgg cctttttgca tgaggaatgg gaaaaggcca cactgcccgg
    13621 ggtggcctga acggaactga gtgcggacaa agcacaccag cgtgacgagt cgtatgatat
    13681 ttcgtggcct gaaccgttag gtcgtcattc ctaagagatg ggaggacaag caacatgaac
    13741 aagcaactgt gtgtgatggt tttggtggct ctttcgctgg gcacaagttt tctagcccgc
    13801 gcggaggaag ggcgtgcccg cgatgagatc atcgatcctg cggtggccgc ccaagacccg
    13861 gattttgcta ttcagggtga atacctgggt gaaggtgtcc tgcccgatgg aacgcagggc
    13921 aagctgggcg tgcaggtggt cgcccgcgga ggtggccagt ttgaggcgtt catcctggtg
    13981 ggcggtctgc caggagaagg ctgggatcgt agccgtcctc gcattgccgg aaaagcggtc
    14041 cgtgaagacg gcaaggcaag cgtccaagca gagggttggc agggcgtgat cgaaaacggc
    14101 gagatgacgc tcaaacacga gaaaggcgaa gtcaaactca agcgagtcga gcggaaaagc
    14161 ccaaccttgg gtcagaaacc acccgaaggg gccgtcgtcc tctttgacgg tcgcaatgcc
    14221 gatgccttcg acggtggcac ggtgcttccg gatgggagtc tcctgtcggg cgtgaccacc
    14281 aagcaaggat ttgggagcta cacgctgcat ctagagtttc gcctctcctg gatgccgcgg
    14341 gccttggggc aagctcgttc caacagtggg gtatatctcc acgactgcta cgaagtccag
    14401 gttctcgatt catttgggct gacgggggaa gataacgaat gcggcggcat ctacagggtg
    14461 aaagcccccg atctcaacat gtgtttgccc ccgctcgttt ggcagacgta tgacatcgaa
    14521 ttcaccgcgc ccaaatatga gaacggccaa aaggttgcca atgcgcggtt gacggtccgt
    14581 cataacggcg tggtcatcca cgacaacttg gaattgccgg gggccacgcc gggacgccag
    14641 caagaaggcc cggccccgcg gccgatccat ttgcaggccc acggcaatag ggtgacctat
    14701 cgcaatatct ggctggtgcc gaaggattga tgtgcaacac ttgtgccagc cggcgcttca
    14761 aaaagcacgt ttcttgccct ggcttacacg tggcaataag gtagaatatt gatgattggc
    14821 caacaagttc atgtgatgag ttgtcggcct gtgaaaaagt gaaagtggat ttttcggcca
    14881 ttgtaaccga taacaatcca cgcaggaaga atcgttggtg aagcagacaa tcggacctct
    14941 cgccgcatat tccgacggag gtgccaaccc tttagcgtga accagggtca agtcaaaggc
    15001 ttgtgcaagt gcacgcccga ccctcgttcg tctcgcagcg acgcatccag tcgcaatatc
    15061 gcgagaggtc gattggtcaa ccgccggggt ccatcttgga ctcgggcggt tttctttttg
    15121 agaccgtcag aattggtccg atcttcgacg acgagcaacc acggtgggtt acccgtacca
    15181 atgcggggcc actgaatcgg acgtctgtgt gccgtggtgg tcgcgataca tggtgggaca
    15241 ttgatcggca gtgtatcggt cgtcacggct cgttttgctt cgggttaaga gcttatccca
    15301 taatctcccc tcgcccgttt ggcgggagag gggtcggggg gtgagggtgc ttcgaaccgc
    15361 tgccggtttt ctcaatcgat tctcacgttc gcttgccctc accccaaccc tctcccggag
    15421 ggagaggggg acttcttggg atagacccta aaggcaacgt gccgcgccgg ttgcaaacgc
    15481 gtcgcctttt ctccacaatg cttcagggaa tcggcgagat tatcgaaatc gctttgatcg
    15541 tctgcacgat gtgtcccgtg tgatgagccc aacgctggag gtttctgata tgcacctggg
    15601 gagagtgata ggccgcttgc tctcggcatg gcacctcgga ttggcctacg gtgttcttgc
    15661 cgtagctctt ttaccgggag cagcgttgcg aggaggcgaa gccgcacaac ccgttgtgga
    15721 agcctccgtg gtggagcaag tagatgtctt catttctggc gagaaaggct atcacaccta
    15781 ccggattccc tcattgctcc gcagcccgga tggtagcctc ttggcctttt gcgaaggccg
    15841 aaagcacggg ctaggcgatt cgggcgacat cgaccttgtt ttgcgacggc tggccccggg
    15901 tcaatcaact tttggggacc tcctcgttgt ctgggacgac ggtcccaaca cttgcggaaa
    15961 cccctgtccc gtggtggatc agcaaaccgg tgtcatttgg ctttggatga cgcacaattt
    16021 gggcgaggat catgagcgag acatcgtcgc ccgcaaaagc cgtggcacac gcacggtgtg
    16081 gcttgccaag agtgttgacc acggccagac atggacccag cctgtcaaca tgacggagca
    16141 cgtcaagttg cctgaatggt cgtggtacgc caccggaccc ggttgtggga ttcaaactcg
    16201 cagcggtcgt ttggtcatcc cctgcgatca tattgcggac gacggcacgt ggggttccca
    16261 cgtcatcttg agcgatgatc atgggcaaac gtggcgtctg ggcggagtgg ctaagcccaa
    16321 gacgaatgaa tgcgaggtcg tcgagctttc ggatggccga cttctcctca atatgcgaaa
    16381 ttacaaccgg cagtttcctt gtcgcgctgt ggccgagagt tctgatgggg ggatcacctg
    16441 gtccgaggta cggtatgacc agcagcttgt ggaaccggtg tgtcaagcca gtatccgccg
    16501 cgtggacgga attactatcg gagggcgacc gttgatcgcc ttttccaatc cggcggacac
    16561 caaggaacgc cgcagaatga cggtccgctt tagcccggac gatggtcaga cctggccatg
    16621 gtcagtcgtt gtttgggaag gtccggcagc ttacagttgt ctggccagtt tgccggatgg
    16681 aaccgtcctt ctcctttatg aacgtgggaa caaacatgct tatgagcgga tcaccttagc
    16741 ccgaattgtg attcgggtaa aatcaccagg ctcataaaca tagagagggc ctgcggtaaa
    16801 tgtgatctgg ggaagcgaag accttcttgg ggggcagcat aagtatgtca gttgaccgga
    16861 ggttgaggca tgttacaacg ttggtttcag cttcgagaac acggaacgaa tgtaccaaga
    16921 gaattcctgg caggtctgac gacctttctc acgatggcct acatcatcgt ggtgcaacca
    16981 caggtcctct ccgggaagat gtttggaaat gagacggggc tggattttgg ggccgtgaca
    17041 acggccacgt gcgtttccgc ggccatcgcc acgttgatca tgggaatcta tgcccggtat
    17101 ccgattgccc tggcgccggg catgggagag aatttcgtct ttgttctctc gttaataccg
    17161 gccgcccagg tttggttgga gcaggagatt caagcgggga gaattcaggc gggaacgacc
    17221 acggcttggt ctattgcttt gggggtcatt ttctattccg gggttttgtt tctcgttcta
    17281 tcgctactcg gaattagaga acgattactc gaggccgtta gtcccagcat gagaaatgcc
    17341 attgcggcgg ggattggcct ttttatcgcc ctgttggggc tgcaaggggc atcggtgatt
    17401 gtgggggctc cggggcagat cgtcaaattg agtccgcgat tgaccagtcc ggatgtgatc
    17461 gtcttttttg tgggtctaat ggtgacggcg gcattctatg cccggcgcat tcccggggcg
    17521 attgttattg gtatttttac ggcaactctt ctggctgtgg gattgcggca ggtgattcca
    17581 ctactaccgg gactggccga aacggagatc gtccggcaat cgcagcttat gacccgattc
    17641 gccccggcag aacatttgtt ttccggtcca ccctcgatgg ccccaacatt tgcgaaaatg
    17701 gatttgatcc atgcccttgc tttgccaatg atcccctaca ttttgatttt cctattcatg
    17761 gatgtgtttg acactctagg aaccttagtg ggtgttagcg aacaggccgg attcattcgc
    17821 gataaccgca tcccccgggc gaaacaggca ctgtccgcag atgccgtggg aactgtggtg
    17881 ggcgccgtgt gtggaactag taccgtcaca agctttatcg aaagtgcggc ggggatcgag
    17941 cagggcggaa gaacaggcct tacggcggtg atcgtggcca tcctctttct tgtggccccc
    18001 tgttgttatc ccttgatcga gatggtcggc agttatccgc cgattaccgc tgccgccctt
    18061 ttgatcgttg ggtcaatgat gatgaagaat gttctcaaaa tcgagtggga tcaggccacc
    18121 gaggcgattc ccgccttcgt caccatgacg ggaattgtct tcagctattc catcgctgac
    18181 ggcttggccc tggggttcat tgtttatccc ctcatcaagt tccttgccgg caagggacgc
    18241 gaagtccctt ggctgatgta tggcctggct gtggttctgt tggcctatct tgtgttcgcc
    18301 aaggcacaca tgggttaatg ctcggacgat gccgggttgg tgatccccgg atgcttgccc
    18361 ccatagtcca cgggtggcgg caatggttat cgtttggaag caatgggcga agctggcaag
    18421 ttaccggctg gcgacataac gccctgttcc ccgacgtacg gcttcgtcaa atgctcgtag
    18481 gcctccgcgg ggtcatgttc tggggccaaa aaggactagc ggcacggtta tccaaccagt
    18541 tgatcgaagt ccttcacggc gatggcggga tgggtgctga agtggatgcc tgtgtcttta
    18601 ctgacgacca ctgaggcctt agcggcctgt tcgattcccg gaaggtccac gatcaggacg
    18661 agatccacct ctcccagcgt ggcgaacatc gcccggacac tacctcctag ttccgcgatc
    18721 gtcttcacga acttttctgt ccgttcggcg ctgatctggc gtaaagcctc cggggaatac
    18781 ttgccgagca tgacgaaggt ggccatagcc ttactccttg tgaaaagaga caacgtcaca
    18841 ccagacttcc tcatggggcc gtccggtgtc cccaccgcaa caatcgaccg tagtttatct
    18901 tcttgactcg gcggaagaaa cccacgtcgt atgcgaaaag aaagtcggct ccgtgattct
    18961 gtagcccggc ggaaagcgac ctttgaggtc atccggctca acggcgaaca gtgggctgat
    19021 ttcgacggcg acaccatcgc agacggcgac ttgattgcgt tccagccaag agcggtgcag
    19081 agcgatcatc tgttgccgga catgctcggg cgtatcgcga tccgcacccg gggcgttttt
    19141 cagcggtgcg aagtgctggg ccgcattgac ttcaatgacg atggcattct tggcatgcgg
    19201 aatgagatcg aaaatgaacc gttcaaactt gatcgcattg ggcttcgtcg gtgtcaccgg
    19261 cccggacgcc ggatcgtagt aagggacctt tttgtaggcc aaatgatagg gcaaggcgtc
    19321 aacctgctcg tacatgcgga agagaaaatc gacgttaatg atgtgaaccc cgatacttcc
    19381 tgcccacaaa agcaaccggc catcgggtga gcgtttttgg gcaagctcgt cgggcaagtc
    19441 gctgtattcg accacgtaaa gccgtccgtc cacctcgatc acatttccca cccgatcgaa
    19501 gggcgtctct ttagcgacaa cctcggtgga catttctgaa ccgaagtaaa tgtgatagcc
    19561 gatcgccgag ggcgaaagaa tatccaccag gggattatcg acctgaaagt aaaaaaggta
    19621 cttgatgccg cgggacgcca gcttctccaa cgtcccactg cggataatgg cagctaacat
    19681 tccaccatgg ccatcgggag agagggctaa atccccgggg gcggaaagca agatcttgcc
    19741 ggtggcagca tccacagagg gcattgtgcc ctgacagaag aagtaaagat cctcttcggg
    19801 cagtccaaat cggccatggc gcgagaagaa ctcgacggtc tcctgatggg tggccgggct
    19861 agtcatcaca gcaagtggga tggggcaacc ggctttccgg gatcgcgcaa ggaccttttc
    19921 cacatggatt tgaaagagag aatgtccgga gattggccca atggggaaca ttcctttcgg
    19981 gtgaggaaat cccaagcgtg tcccctgacc acccgccacc agcagtgccg ccacctcacc
    20041 gcgttggagc acaccttggc ctaagcgata ggcgtcggcc ggggaaagtc ccgcaccttc
    20101 agaatccaaa cgaacacccc gtggggatac cgcgcgttcg acgaggctgg cgatatcaat
    20161 aggcagattg cggtcccgaa agagtcgcgc aatgaggggt aaatccaact cgcggatctg
    20221 cgcagccagc cttgcccgcc cttcctccga caggtcttcc caacctgcca aaagatgcgt
    20281 ctgctggaac ggctccagca aggccttgag atccttatat tcccacgttt ttcccatcct
    20341 ggaaacctgt tgttgcgttc ggaccaaaga ctgaccgatc ccgcnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnntatttt tctcttcaag actatgagtc gccgcgaatc
    20521 cggggtaaaa tcagagggta cctgggcggc cgcgagcatg ctcgtttttg gggcataaat
    20581 agttgtgttc tcattttacg gtcaaggccc ggcatcacga agacctaata aagtattcct
    20641 taaagaaaaa gggcgtgcca atggtgttga catttttgcg gtttgctttg ttgatcgtcc
    20701 tagccggcgt tgccgttatt gtggtcaagt ccggccttta tccgcaggat catcagtgga
    20761 tggcctgggt ccttgtggcg gtgatcatgg ccatcggcgg acttgccatc cttgtggacg
    20821 ttctgattcc ccggaagccc ctagagatca tcacctcggt ctattttggc ttgatcgtgg
    20881 gcttgttcct cagctacgta gcgggcttgg ccttgacgcc cgtctttgag agcctacaag
    20941 tggacgagca agtccgcaat gtcgtccagt tgattgtctc ggccatcttg tgttacacgt
    21001 gcatcagctt tttgtttcag accaaggacg attttcgctt tatcatcccc tacgtggagt
    21061 ttaccaagga ggtgagaggg cggcggcctt acattctgga cacgagcgtg attattgatg
    21121 gccgaatcgc tgacctggtg gaaaccaacc ttttcgatag ccgactcatc atgccgcggt
    21181 tcgtcctaac agaattgcag gcgattgccg acagcgccga ccgtaccaag cgggcgaggg
    21241 gccgacgtgg cctggatatc ctcaatcgtc tccgtaacaa tccggatgtc gattttgtca
    21301 tttgggaccg tgatctgcca gagtttagtg ggcagtcggt ggacctgaaa ctaatcaccc
    21361 tggcaaagca cctggaaggt cgccttgtga caaacgatta caacctcaat aaggtggccc
    21421 gagtccacgg cgttcgggtg atcaatatca acgacctagc caacgccctc aaaccgcggt
    21481 ttctgccggg tgaacaaatt caagtccgtt tggttcgccc aggggaggaa gagcaacagg
    21541 gtgtgggtta tcttgacgac ggcacgatgg tcgttgtgga ggatggtcga gaatacgtgg
    21601 gccgcacggt cgtcctcaat gtgacgagcg tcctccaaac cagtgctggg cggatggttt
    21661 tcggacgcta cgaccgcact gtcgaacaca accacgaagc ccgcgaggac cgagttgccc
    21721 gctcgtgatc gaagatatga taaccgcgcg gcagcgccat cgtgttcggt aagagcctat
    21781 cccataatct cccctcgccc gttcggcggg agagcgggcg ggggtgatgg tggctcgaat
    21841 tgctgcggat tctctcgatc gattccgcca tacgcttgcc ctcaccgcaa tcctctcccg
    21901 gagggaggga actttgggat aggcgctaaa tgacggacgc gggggaccgc gtccctccct
    21961 gccctcaccc caaccctctc
//
LOCUS       DAOSUT010000063        21908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1548_length_21908_cov_9.533611, whole genome shotgun sequence.
ACCESSION   DAOSUT010000063 DAOSUT010000000
VERSION     DAOSUT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21908
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_21908_cov_9.533611"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1167)
                     /locus_tag="PLD05_07525"
     CDS             complement(142..1167)
                     /locus_tag="PLD05_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77325.1"
                     /translation="MSRCVNVCALVTCAMVASFVLNRSSGAEEGQIRKLGLIGLDTSH
                     VIAFTDIINAKDAEGVLAEFEVVAGYPNGMPDNPSSWNRVEEYTSKLRDKGIKIFDTI
                     EEMLPHVDAVLIESVDGRPHLEQARKVISARKPIFIDKPMAASLCDVLLIFRLAEEAK
                     VPVFSSSSLRFSSGFQAVRKGEAGFGTVKRCVAWSPLHIEPHHPDLFWYGIHGVETLY
                     TIMGTGCQTVTREAENRVVGTWKDGRIGVFEGRNGYGAEIEGSERSGSAGTYEGYKPL
                     VIEICKFFKTGVPPVAKEETIELFAFMEAADESKRLGGQPVAIDQILAKAQAEADKKY
                     QLISDCQ"
     gene            complement(1226..2185)
                     /locus_tag="PLD05_07530"
     CDS             complement(1226..2185)
                     /locus_tag="PLD05_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77326.1"
                     /translation="MSLNATMTRRRFVETSSALVAGLSWRVPAWGAETSATIKKAMIG
                     KPTEETLKQLKAAGFHGIESNDRHADLKDAESARKLAESLGMRIHSVLYGWANFNTDS
                     VNSDIESVARSLETAAAYGADALLLVPCRIGGMPMPEAWEFDIEFDEKTGHVLRVVKG
                     DNSPYRTYIEAHNHAIDTSKEAVKKLIPVAEKTKVIIALENVWNNLWVKPAIFAHFVK
                     SFDHPMVQAYFDIGNHVKYAPPVEWIRALGKTIVKLHVKDFLLNPDGRGGKFVHPRDG
                     SVDWPAVRAELNKIGYTGWMTIEDSGLSLEEFSQRLDMIIAGK"
     gene            complement(2291..4972)
                     /locus_tag="PLD05_07535"
     CDS             complement(2291..4972)
                     /locus_tag="PLD05_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP77327.1"
                     /translation="MRALPCLAVALSCGLVWANDVVWLETEKFADRGGWVNDAQFVDQ
                     MGSPFLLAIGLEGPVADATTTVVIPATATYRVWVRTRDWLPEYSPGRFQVILGEQTVE
                     RVFGANKQERWVWEDGGTVRLQAGPLTVRLHDLTGHYSRCDAIVLTADPNFRPASELG
                     QLARQREMFGGVSREIKTLEPYDTVVVGGGLAGTFAAVASARMGCRTALIQDRPVLGG
                     NSSTEILVQPQGDTTREPLDPGEGGIIEEVRGDVFGYSERLLRLVRNQPHLDLFLNTH
                     ATGVEMESPKRIKAVIALDVNTGERYRFPAKTFIDCTGDGVIGVWAGAEYRHGPEPRS
                     MYNESRAPEQGEVNKGTMGGTLRYMTEQLAQPVEFKAPDWAHKFHDCSDFNPGRHPQL
                     QFGGWQWVIEYGGELDTYHDAEVIRDELLRIIWGMWDHAKNHCPKLKEEAKNCKLTWV
                     SYVVGKRESRRLIGDYIMTEHDIANQTLFPDRVAYGGWGIDIHPPKGFYDPGPPAIFS
                     HKVKFSIPFRSLYTKDVDNLMMAGRCISVTHAALGATRVMITCGLQGQATGTAAGFCK
                     MHDTTPRGVYERYITDLQQQLLKDGCYIIDLPNQDPRDLARSAKVSASSVAPNEVYSV
                     ASFLPVHKLDHDRAVMFTWQGGRLDAIKVYLNSERSDSVRLTGHLCVATGLGEFNGAE
                     EIAQIEAEVLPGKREWVSFGVNRDLPKGYYYLWLPKTSGVSWSLFAQRPAGTSRAYRG
                     AGAWTPMPDCYAFQLVPGETPQLPLAGEDSIAYFAPENVIDGFARAIRGRPHSWRPDP
                     KLAWPQWVALEFPQSVSFNTVHVSFQSASMRADDFDIEVWRNDQWVRIVEVRGNTERR
                     RVLTFPRVTAQKVRLVIHRAQPDMGVCEIRVYDESEQ"
     gene            5211..6722
                     /locus_tag="PLD05_07540"
     CDS             5211..6722
                     /locus_tag="PLD05_07540"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77328.1"
                     /translation="MKGVRVSRRDFVDAAVTATVATIVPRSVLGGPKFVPPSEKIHIG
                     YVGCGTQGLRQLMSALKHPGVRIVAVCDPNRKSDDYPEWGRGELVGKIRAFLDDPDWG
                     AGARGGLCGREVGLEVVSRYYAKEANRASDSNPACRAYSDFRQMLADEKDLDAVYIMT
                     PDHLHGVVAARALKAGKHAITHKPISNVWAEVRFLRDLAKETGLATQLFCAAGQADSS
                     TIAEWIAAGAIGTVREVHNWSTRPFWPQGMTEYPSEVVPVPDGLEWDLWLGPAAERPY
                     HPAYTHAVFRGWYDFGTGALGDMGHYSFYQIFKILKLGCPVSVEATREQFWKIENFTW
                     KKQINRVSYPRASIIHWEFPAEGNRPAITLHWYDGGLRPPLLEELEVDGREMPAEGML
                     FVGDEGKILAGFTGTQPRLIPEKKMKEFQPPPQTLPRPKEEFEQFVLACRGEAKPDAN
                     FESVYPMAETILLGTIAVRVEKKLKWDSTKGEFLNSSEANALRTRENRKGWEL"
     gene            complement(6741..8297)
                     /locus_tag="PLD05_07545"
     CDS             complement(6741..8297)
                     /locus_tag="PLD05_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOP77329.1"
                     /translation="MDLITILVFLGLALLVWDCIEVGRNDASNIVNAVFGARILNRRT
                     AVRVAGLAVVIGASAASPVFETARKGIFDPGMLTLEQAVIAYISVYLVDTVLLFTYSA
                     FGMPVSTTACLIFELVGASVGLYGLSVVHWGKVGTVIAAIFVSIFMTGLAGFLIQRVF
                     RAAIGTKIDDRETILLHGPWISGFMLTGLGWFMVFKGMSAIPFVNAFREYAIDPYIDA
                     FTPTFAFLSAWGLFTLIVHLFLSFSGTIGTRYLFPAMAVLGMICLAFAFGQNDLANCA
                     SPGISAFWLWRHAEQTVAQATQITIPTWVLFICGCLLVAGMMTENAQRVTRAQVNVGS
                     QFDRVALYAPEWCRAMARALLRFFPHRPELAPPPMVSPQGKKVHYDALRAAVISSVSA
                     GVIALASSRGLPVSTTYVAFAAVIATGLADRVLARGDADLKIGRAIWVVVSWFLAAII
                     AVVATAGVARLIYHLGFVGLLSALAINLSVRFYSQKVADEQERRIHRHRGGQPPVDQL
                     AKTEIHADVP"
     gene            8628..10562
                     /locus_tag="PLD05_07550"
     CDS             8628..10562
                     /locus_tag="PLD05_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOP77330.1"
                     /translation="MDRQQVRTTWEKACDELIRRRESAGYWRGCLCSSPLATATAISA
                     LAVYSRSLESKGGSFSPTMAESIQSGLRYLLATQRPDGGWGDTERSLSNIATTYLVRA
                     AIELGGKAMVPEDALRRADQYIERQNSENGLRQRYGRDQTFVAPILTNVALAKLCSWR
                     SVPQLPFELAAFPQSLYRFLGLPVVSYAVPALVAVGLARFHLGPKGNTVVACLRRAVT
                     PKCLAVVETMQPQSGGFLEAVPLTAFVTMSLSAIGLAGHSIVQKAVRFLLSLQRSDGS
                     WPVDVDLATWVTTLATNAIAAGEYPGWMNLVSLDWILGCQHRDFHPFTGADPGGWGWT
                     DHSGSVPDADDTSGALLALANFWQKGDAETRRKILPSALLGLDWLVRLQNKDGGWPTF
                     CRGWGKLPFDRSAVDLTAHAVRAFHTWYNLLMGQPPVDLAGGRIVFDELWPDAAARRL
                     GRLENIDRALLPRLKRSISKGVAFLRQKQEHDGRWLPLWFGNERLPNEENPVYGTSRC
                     LMAYVALNQQHNPAAEKAIAWLLQAQNRDGSWGRSASEEPPRRDDETSPDAPGSIEET
                     SLAISALASFRDNPAVPAALERGLSWLTWNIENGNWRIATPIGLYFARLWYYEDLYPI
                     VFSVEALGRYLRHCDQEEKT"
     gene            10640..12388
                     /locus_tag="PLD05_07555"
     CDS             10640..12388
                     /locus_tag="PLD05_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOP77331.1"
                     /translation="MGEELVRSQETTVQDLGSGESIKVVPQDRSCREQIRREAIRLAE
                     QLNHSELLTRDKIELTAKIVLQNLGLPQSYLGWTMVAVGSAFWRSQVMRVPYHRRLLL
                     LPHCMRNLSLCAGSYNAEGLQCASCGGCPLGWLRDVALSLGYRVLIAEGSPIVLRLIL
                     SGEADALLGVACLNSLERSLERVLMAGVPSMAVPLIVGGCRETVADEDWILEMIETPY
                     IDAESSGRSYLPLMRLAAQLVESELDGLAPRIFPTTEQREDAKVLSITEDIAYQYVKD
                     GGKYYRPFIVLSVYDALSGGKHAHRNHGDAATTMPTAVRRVALAVEVFHKASTIHDDI
                     EDDDSYRYGRPTLHKRWGIPVAINVGDYLIGLGYALLAETQDEMGKDVVADLVTLFAR
                     AHRNLCTGQGSELWLAQRPELAFTVPQVLRIYSLKTGTAFEVALLAGMRLAGEMPSWW
                     KLVRQFARAFGIAFQIQNDCQDWQGQEHNKLRHGTDAVALRPTIFSALAWHTTPGEFA
                     ALIEKCQSKREAKHEDIARLFQEFFRKHGLFNTVQEMLYLERTRALEIADKIPHENLR
                     IFLRYLTESAIRPTLE"
     gene            12574..13500
                     /locus_tag="PLD05_07560"
     CDS             12574..13500
                     /locus_tag="PLD05_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOP77332.1"
                     /translation="MRNSIDLFFHFVTRSAHLSEEMRLRHRQWLVGQQRPDGGFAGRE
                     GDSNLYYTGFGARLCAILWSDVTDPWVSMTQYVAMQLEAIDSAVDLASAATIYALVRL
                     LVDESLAQSLEDRLRARAELLFARFYRPDGGFAKSEQSGFGSTYATFLALNTMNLLML
                     PLPDREQIVQFVCSRQRPDGGFAEVPHAKNAATNPTAAAISILADLEALDKIDLQQVI
                     EYLFRMQRADGGFSAGPHAPVSDLLSTCAALSALWLLDRSEFRRLDVERVERFVQSLE
                     NATGGFRGCRLDDQSDVEYTFYGLATRALLHS"
     gene            complement(13558..14952)
                     /locus_tag="PLD05_07565"
     CDS             complement(13558..14952)
                     /locus_tag="PLD05_07565"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOP77333.1"
                     /translation="MNKTCNMLARLMIWFLCVAFICADVIASDRDNGYRGIWFTLGQW
                     TEFGDKYSGGLGTYTANHVPIAIYCKEVNKTFFVYGGAKEGKRHLLNMVGYFDHTRGV
                     VPRPTIVHDKNGVDDPHDNAALAIDPQGYLWVFISGRARSRPGFIYRSEKPYSIEHFE
                     LIKEGEMTYPQPWWLPDRGFLYLFTKYTKGRELYFSTSPDGFQWSEEIKFAGMGGHYQ
                     TSGRKDQRVFTAFNYHPGGNVDRRTNLYYIETKDVGATWTNIQGEVLTLPLSDPINPA
                     LVREYEKEGRLVYIHDVDLDSQGYPVILYTTAASYKPGPEGDPRYWTIAHWNGTNWEF
                     SEITQGNHNYSTGALYIEGDVWRVIGPTERGPQPIGAGGEIAIWKSDDKGKSWQKVRD
                     VTRGSAFNHNYVRRPVNAHEDFYGFWADGNPDTFSPSCLYFCSRDGSRVYRLPYHMDT
                     EEAVPELLPPLETK"
     gene            complement(15197..16090)
                     /locus_tag="PLD05_07570"
     CDS             complement(15197..16090)
                     /locus_tag="PLD05_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOP77334.1"
                     /translation="MHKAVIVGCGYVGSYVGRLWLKKGYCVCAVTSSSERVAPLEAQG
                     FEVVVADVLDPTSLERLPGGDVILYSVGPTGHRDVPRWRLYKEGLDNILTRFRHTTSQ
                     IGLVSSTGVYGNHPEGWIDEQTPRNPQRDSARALAAAEDLLLESSWGDRAYIFRLGGV
                     YGPGRIPLVSQLVQGQPLPTSPDAMLNLIHVEDAATAIDLVISRGQPPCIVNVVDGFP
                     VSRRDFYNELSRLLQVASPRFAESLPAFETDQVSSAARRHSPVTKRVSNQLLVKGFGM
                     SLRYPDYRSGLAEIVRQGFSQ"
     gene            complement(16269..16802)
                     /gene="ruvX"
                     /locus_tag="PLD05_07575"
     CDS             complement(16269..16802)
                     /gene="ruvX"
                     /locus_tag="PLD05_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOP77335.1"
                     /translation="MARSFEDEHPQLPSTPEIPAKGRIAGIDFGKTRIGIALSDPGRV
                     LATPWAVYQRRGPQQDAEYFRKLVKQEEIVLFVVGLPVHCDGSESESSQAAREFGHWL
                     EAVTGTPVVFMDERFSTQTADTWLRSAHLSHKRRRERLDKVSAQIILSAYLATYKPSS
                     GPPQSWSPSPLDDGESV"
     gene            complement(16879..17661)
                     /gene="nagB"
                     /locus_tag="PLD05_07580"
     CDS             complement(16879..17661)
                     /gene="nagB"
                     /locus_tag="PLD05_07580"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOP77336.1"
                     /translation="MEVIIKKSYEELCQAAAQAVAQVLNSKPNAVLGLATGNTLVGLY
                     RELVRMFREGEIDFSQVVTFNLDEYVGLRKEHPQSQHYFMHEHFFKYVNIAPQNIYMP
                     SGTSQNYQAFCRWYEQRIKECGGIDLQILGIGTDGHIAFNEPGSSLGSRTRIKTLSRR
                     RLEEIKDLFDEGEELPIYAITMGVGTILEAKRIILLAHGYAKADAVAAAIEGPVTSMV
                     TASALQLHRDAIAYLDDEAASKLKLYEYYVWVQEKRPAAPGM"
     gene            17957..18745
                     /locus_tag="PLD05_07585"
     CDS             17957..18745
                     /locus_tag="PLD05_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOP77337.1"
                     /translation="MSNSTISTQRLALVTGGSRGIGAAICTKLGELGFHVFVNYVHNQ
                     EAANKVAQDICEKGGTATVLPGDVGSESDRRRMLSVIREYQRLDVLVNNAGITSVGRR
                     DILEATEESWEKVFNTNLKGPFFLTQAIANEMIRWIREGRQPQGYIINVSSISAFAVS
                     TNRADYCLTKAALAMMTQLFAQRLADEKIQVFEVCPGVIASDMTAPVKEKYDRLIAEG
                     AWPIRRWGEPEDVALAVAALVQGYFPFSTGNRFLVDGGFHIRTL"
     gene            complement(18758..19717)
                     /locus_tag="PLD05_07590"
     CDS             complement(18758..19717)
                     /locus_tag="PLD05_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOP77338.1"
                     /translation="MLRFHFCSRIGAMRSLVGRCLVVLGFCWLLSSGGHSLTWAQDRP
                     LRIIVFGAHPDDCEIRAGGTAALWARHGYKVKFVSVTNGDIGHWGIAGGALAQRRMRE
                     VQEAAKILGITTEVLDIHDGELLPTLENRRIMTRLIRAWDADIVIGHRPNDYHPDHRY
                     VGVLMQDCAYMVAVPFFCPDVPPLKRNPVFLFSYDSFQRPNPFRADIVVAIDEVIDQK
                     LDALVVIESQFVEGGALGSAEAAPKTEEERQKKREQVRESFRQRFARIADQYRDRLIE
                     LYGPEKGRAVKYAEAFEICEYGRQPSAQEIRQLFPFIGQSDSK"
     gene            complement(19989..20360)
                     /locus_tag="PLD05_07595"
     CDS             complement(19989..20360)
                     /locus_tag="PLD05_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77339.1"
                     /translation="MRTVFPTNALENVSPRRDHRKTRCRETVFERHKIDEKRFGMLMS
                     HRKDDKTRRAFFQYVLHTAAIVSHEKTRQGRQLVARLLQVTPSIDQAQLPYSAHRLED
                     YGWRTCALPPQFDCRNAHMTF"
     gene            20812..21891
                     /locus_tag="PLD05_07600"
     CDS             20812..21891
                     /locus_tag="PLD05_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOP77340.1"
                     /translation="MTKNPITREEILRGLDGFQDPELGRSIVRLDQVQDVQISDGLVK
                     IVLGLTSFSAPLWPELREEVRNLLRTQLTPDVDWEVVIREFPRPAEKLGTIGLTAKYV
                     IAVGSGKGGVGKSTIAAALALGLDRAGCKVGLLDADVYGPSIPHLLGSDERPTMYEGK
                     IIPITVGNLRVMSMGFIVPKDEAVIWRGPMLHGAVQQFLRDTDWGDLDYLVVDMPPGT
                     GDVAISLSQLLPVSGAVVVCTPQELALLDAVKAITMFRKVNIDVLGIVENMSYFICPN
                     CGTRHDIFSSGGARKKADELGLPFLGEVPLNVMLRIKGDEGRLVECLAEENIGPYFDA
                     ICKNVVRQIVNQRRGTPPLPSLSVL"
BASE COUNT         5063 a         6116 c         5859 g         4870 t
ORIGIN      
        1 aagcggcccg aaactggacg ccagttactg ctgcacatac tgctgcagta agtaggtgta
       61 atgcgtggac acaacgtgac aggggcgaaa acactatttg cccgaaccat aatcctgaca
      121 ataagttgca acttaagtca tttactgaca atcgcttatg agttgatact tcttatccgc
      181 ctctgcctgg gccttagcga ggatctggtc aatcgcgacg ggttgcccac ccaagcgttt
      241 gctctcatct gccgcctcca tgaatgcgaa cagctcgatt gtctcctcct tggcgacggg
      301 cggcacacct gtcttgaaga acttgcagat ctcaatcaca agaggcttgt acccctcgta
      361 ggtacctgcc gaaccactcc gttcactgcc ctcgatctcc gcgccgtagc cgttcctgcc
      421 ttcgaaaact ccaattcgcc catctttcca agttcccaca actcgattct ccgcctcacg
      481 tgtcactgtc tgacacccag tccccatgat cgtgtaaagg gtttccacac catggatgcc
      541 ataccagaag agatccggat ggtgtggctc gatgtgcaac gggctccagg ccacgcagcg
      601 tttgaccgtt ccgaagcctg cttcaccttt gcgaaccgct tggaaacccg agctaaatcg
      661 taaggaggaa ctggaaaaca caggaacctt ggcttcctct gccagccgga agatcagcaa
      721 tacgtcgcaa agggatgccg ccataggttt gtcgatgaag atgggcttgc gggcggagat
      781 gacttttcgc gcctgctcca ggtggggacg accatcaaca ctttcgatca ggaccgcgtc
      841 tacatgcggc aacatttctt caatcgtgtc aaatattttg atgcctttgt cgcgaagctt
      901 gctcgtgtac tcctcaacac gattccaact cgaggggtta tcaggcatac cattggggta
      961 accggccaca acttcgaact ctgcgagcac gccctcggcg tccttggcgt tgatgatgtc
     1021 cgtaaaagcg atgacgtgcg aggtatcaag cccaataagc cctaatttcc gaatctgacc
     1081 ctcctccgca cctgaggatc ggttgagcac gaaagacgca accattgcgc acgtgaccaa
     1141 tgcgcaaaca tttacgcacc gagacatcgt tgtatctcct ttaagcacaa caaacggtgt
     1201 tcattcacac agtgcgaagt ctatgttatt tccccgcgat aatcatgtct aagcgttgac
     1261 taaactcttc cagggaaaga ccactatcct cgatggtcat ccagcccgtg tagccgatct
     1321 tgttcaattc cgcccgcacg gccggccaat caacgcttcc atcccgcggg tggacaaatt
     1381 tgccgccacg accatcggga ttcaacagga aatctttcac atgcaacttg acaatagtct
     1441 ttcccagtgc gcgaatccac tccacgggcg gagcatactt cacatggtta ccgatgtcga
     1501 aatatgcctg aaccatgggg tggtcaaacg acttgacaaa gtgggcaaaa atcgcgggtt
     1561 tcacccacag gttgttccaa acattttcca aggcgatgat aaccttggtt ttctcagcga
     1621 caggaatgag ctttttcacc gcctccttcg aggtgtcgat tgcgtggttg tgggcctcga
     1681 tgtaggtacg atagggactg ttgtcacctt tgacaacgcg aaggacgtgc cccgtcttct
     1741 catcaaactc gatgtcgaac tcccaagcct ccggcatcgg cattccgcca atgcgacaag
     1801 gaaccagaag aagggcatcc gccccatagg cggcagcggt ctccaaagag cgggccacgc
     1861 tttcgatatc ggaattcaca ctgtccgtgt tgaagttggc ccagccgtag aggaccgaat
     1921 gaatcctcat gccaagcgac tcggccagtt tgcgggcaga ttcggcatct ttcagatcgg
     1981 catgtcgatc attcgattcg atcccgtgaa accccgccgc ttttaactgc ttgagggttt
     2041 cctcggtggg cttgccaatc atggcctttt tgatggttgc agaagtttcc gcaccccacg
     2101 ctggcacccg ccaggaaagt ccggctacca aggccgaaga cgtttcaaca aaacgacgac
     2161 gcgtcattgt ggcgttaagg ctcatagctt ttctcccttt ttgcaaacgc caaaggtttt
     2221 ctaaacccga ataggcaaga cggctgccca gaatttacgt ttcacaagcc ctcagccagt
     2281 gttcacatgt tcattgttcc gattcatcat aaacgcgaat ttcacacact cccatgtcag
     2341 gttgtgcccg atgaatgacc agccgcacct tttgtgcggt caccctcggg aaggtcaaaa
     2401 cccgacggcg ttcggtgtta ccccgaacct cgacgatgcg cacccattgg tcgtttctcc
     2461 agacctctat atcgaagtca tcagcccgca tgctcgctga ctggaacgac acatgtaccg
     2521 tattgaacga aaccgactgc gggaattcca gcgcaaccca ttgaggccag gcgagcttag
     2581 gatcaggccg ccatgaatgt ggcctacccc tgattgcccg cgcgaaacca tcgatcacgt
     2641 tctccggagc gaaataggcg atcgagtcct cgccggcgag gggcaactgc ggtgtttcgc
     2701 ctgggacaag ctggaaggca tagcagtctg gcataggtgt ccatgcgccg gccccgcgat
     2761 aagcccgaga tgtgcccgcc ggacgttgtg caaagagact ccacgagacc ccggaagtct
     2821 taggtagcca caggtagtaa taacccttgg gaagatcacg attcacacca aaggagaccc
     2881 actccctctt ccccggcaag acctctgcct cgatctgggc aatctcctcg gcaccattga
     2941 actcgcctaa gccagttgcc acgcagaggt gtcccgtaag ccgaacactg tcagatcgct
     3001 cggagttcag atacaccttg atcgcatcca gtcgtccgcc ctgccaggtg aacatcaccg
     3061 cacggtcgtg atcgagttta tgaacgggca agaagctcgc caccgaatag acctcgttgg
     3121 gagccacgct ggaggcggaa acctttgccg agcgagccaa gtcacgcgga tcttggttag
     3181 gaagatcgat gatataacat ccatctttca aaagttgctg ctgcaaatcg gtgatatagc
     3241 gttcgtaaac gccacgtggg gttgtatcgt gcatcttgca aaaaccggca gccgtccccg
     3301 tggcctgacc ttgaagaccg caggtaatca tcacgcgggt ggcccctagc gctgcatgcg
     3361 ttaccgaaat gcaccgcccg gccatcatga ggttgtcaac atccttcgta taaagactcc
     3421 gaaatggaat agaaaacttg actttatggg agaagatcgc cggtggcccc ggatcataga
     3481 aacctttggg gggatggata tcaatccccc accccccata agcaacgcgg tccgggaaga
     3541 gcgtttgatt ggcgatgtcg tgctccgtca ttatgtaatc gccgataaga cgtcgcgatt
     3601 cccgttttcc gaccacataa ctcacccagg ttaacttgca attcttagct tcctccttca
     3661 gcttggggca gtggtttttg gcatgatccc acatacccca gatgatgcgg agaagctcgt
     3721 cgcgaatgac ctccgcatca tgatatgtgt ccaattcccc accgtattcg atcacccatt
     3781 gccacccgcc aaactgcaat tggggatgcc ttcccggatt gaaatcggag caatcgtgga
     3841 acttatgcgc ccaatcaggg gctttgaatt cgactggctg ggccagctgt tcggtcatgt
     3901 agcgaagtgt tccccccatc gtacctttat tgacctcacc ttgttctggc gcccgcgact
     3961 cgttgtacat tgagcgcggc tctgggccgt gacggtactc cgcccccgcc caaacaccga
     4021 tcaccccgtc tccggtgcag tcgatgaagg ttttggccgg aaaacgatag cgctcgcctg
     4081 tgttaacgtc tagggcgatc accgctttga tccgtttggg cgattccatt tccaccccgg
     4141 tggcatgagt gttcaagaaa agatccaagt gtggttgatt cctcactagc cggaggagac
     4201 gctctgaata gccgaagaca tctccacgga cttcctcgat aataccgccc tccccaggat
     4261 caagcggctc gcgagtcgta tcaccttggg gctgaaccag aatctccgta ctgctatttc
     4321 cccccagcac gggtcgatct tggatcaagg ccgttcgaca tcccatcctg gcggaggcga
     4381 cggcggcaaa cgtgccggca agaccacccc ccacgaccac cgtgtcatat ggttccagcg
     4441 tcttgatttc gcggctcaca cccccaaaca tctctctttg tcgtgccaat tgtcctaact
     4501 cactagctgg acggaagtta ggatcagccg tcaggacaat cgcgtcgcac cgagaataat
     4561 gccccgtcag gtcatggagt cgcaccgtaa gcggtccggc ctgcagtcgg acggttcccc
     4621 catcttccca cacccatctc tcttgcttgt tggcaccgaa aacccgctcg acggtctgct
     4681 cgcccagaat cacttgaaac cgtccagggc tgtactccgg caaccaatcg cgggtccgga
     4741 cccacacacg ataagtcgcc gtcgcgggaa tcacgaccgt cgtcgtggcg tccgccaccg
     4801 gtccttcaag cccaattgcc aataggaagg gcgaacccat ctgatcaaca aactgggcat
     4861 cgttgaccca accgccgcgg tccgcgaatt tctcggtctc caaccagacc acgtcgttag
     4921 cccaaaccaa accacaactt aatgctaccg ccaagcaagg cagagctctc atcaggcatc
     4981 tcatggtgtt cttcctttgg gcaggatctc aaacggaaaa aggtttcaat gttaatcgcc
     5041 acgtgcttac gcatcatacg tgcataagca cgttacttga cggtaaagcc atagtcaaga
     5101 ggccgaccaa ttttctcgcc ttaggcttcc ggttatcata gggcacggtc gaggtttttc
     5161 gccattaaac ggtccactac gaacaaacgt gcaggaaagg gatgcgaacc atgaaaggcg
     5221 tgcgagtctc ccgacgtgat tttgtcgatg ccgcggtcac ggcaactgtg gcgaccatcg
     5281 tgccccgttc cgtgctgggt ggccccaagt ttgtcccgcc gagtgaaaaa atccatatcg
     5341 gctatgtggg gtgtggaacc caagggttga ggcaattgat gagcgccctc aaacaccccg
     5401 gcgttcggat tgtggccgtg tgtgatccca atcgaaagag tgacgactac ccagagtggg
     5461 gccgggggga actggtgggg aagattcgag catttttaga cgatccagac tggggcgccg
     5521 gtgcgcgcgg cgggttgtgt ggccgggagg ttggcctgga agtcgtctcg cgatattacg
     5581 ccaaagaggc caaccgagcc tccgacagta atccagcttg ccgggcgtac tccgacttcc
     5641 ggcagatgct ggccgatgag aaagaccttg atgccgttta tatcatgacg ccggaccatc
     5701 tccatggcgt tgtggcggcc agggcgctca aggccggcaa gcatgccatc acgcacaagc
     5761 cgatctccaa cgtttgggct gaggttcgtt tcctgcggga cctggccaaa gaaacggggt
     5821 tggccaccca acttttctgc gcagcaggtc aggccgattc ctcgacgatc gccgaatgga
     5881 ttgccgcagg ggccatcggc actgtgcgtg aagttcacaa ctggtccacc cgtccgttct
     5941 ggccacaggg aatgaccgaa tatccatccg aggtcgttcc cgttcccgac ggtctggaat
     6001 gggacttgtg gctgggcccc gccgcagagc gaccgtacca tccggcttac acccatgccg
     6061 tttttcgtgg atggtacgac tttggcactg gggcactcgg cgatatgggt cattacagct
     6121 tttatcagat tttcaaaata ctcaaactcg gctgtccggt gtccgtggag gccacacggg
     6181 agcagttctg gaagatcgaa aacttcacct ggaaaaaaca gatcaaccgc gtgtcgtatc
     6241 ccagggcatc catcatccac tgggaattcc cggccgaagg taaccgaccg gccataacgc
     6301 tccactggta cgacggtggt ctgcgcccgc ccttactgga agaactggaa gttgatggtc
     6361 gtgagatgcc cgcagaaggg atgttgtttg tcggagacga ggggaagatc ctcgcaggtt
     6421 ttaccggtac ccagccccgg ctgataccgg aaaagaagat gaaggagttc caaccgcccc
     6481 ctcaaacttt gccccggcct aaagaggaat tcgagcagtt cgtcctggcc tgccgtggtg
     6541 aggccaaacc agacgctaat ttcgaaagcg tttatcccat ggcggagacc atcctgttgg
     6601 ggaccattgc ggtgcgtgtt gagaaaaagt tgaaatggga ctcaaccaaa ggcgagttcc
     6661 tcaattcctc agaggccaac gctctgcgaa cacgagaaaa tcgcaaagga tgggaacttt
     6721 gaggcacgcg tggggttcca tcacggtaca tcggcatgaa tctcggtctt ggcaagctga
     6781 tcgaccggcg gctggcctcc gcggtggcgg tgaattcggc gttcctgctc atccgctacc
     6841 ttttgtgagt agaaccgtac ggaaagattg attgcaagtg cgctgagaag tccgacgaaa
     6901 ccgaggtgat aaatcaaccg tgcgaccccg gccgtagcaa ctaccgcaat aatcgcagct
     6961 aaaaaccaac tgacaacaac ccaaatggcc cgaccgatct tgagatcggc atcgccgcga
     7021 gccagcacac gatctgccaa acccgtggcg atgactgcgg caaaggcaac gtacgtggtg
     7081 gaaacaggaa ggccacgact ggacgccagg gcaatcaccc cggcgctgac ggaacttatg
     7141 acggctgccc gcaaggcgtc ataatggacc tttttcccct ggggacttac catggggggc
     7201 ggggccaatt caggtcggtg aggaaagaac ctcaatagcg cccgggccat tgcgcgacac
     7261 cattccgggg cgtacaatgc tacccggtca aattggctcc ccacattcac ttgcgcccgg
     7321 gtgacacgtt gtgcgttttc ggtcatcatt ccggcgacga gaagacaacc gcatatgaat
     7381 agcacccacg tgggaatggt aatttgtgtc gcttgtgcaa cggtctgttc cgcgtggcgc
     7441 caaagccaaa atgccgaaat gccgggcgac gcgcaattgg caaggtcgtt ctggccaaag
     7501 gcaaatgcca ggcatatcat tcccagaacc gccatggctg gaaaaaggta acgcgtcccg
     7561 atggttcccg agaaggagag aaagagatgg acgatcaggg taaacaggcc ccatgccgac
     7621 aaaaaggcaa aggtcggtgt gaaggcatca atataggggt caattgcgta ctcccgaaat
     7681 gcattaacga agggaatggc tgacatccct ttgaacacca taaaccatcc caaaccggta
     7741 agcataaaac cggaaatcca cggcccatgg aggaggattg tttcacgatc gtcgatcttg
     7801 gttccaatgg ctgcgcgaaa gaccctttgg atcaggaacc cggcaagccc cgtcatgaaa
     7861 atcgaaacaa aaatggccgc tatgactgtt cccaccttac cccaatgaac tactgagaga
     7921 ccgtaaagcc ccactgaagc acctaccaat tcaaagatta aacatgccgt agtgctcaca
     7981 ggcatcccga aggcggagta ggtaaaaagg agcacagtat ccaccaaata gacgcttatg
     8041 taggcaatga cggcctgctc aagggtcaac atgccgggat caaagatacc cttgcgggct
     8101 gtttcgaaga cgggactggc ggccgaggcc ccaatcacca cagccaaccc agcgacacgg
     8161 accgccgttc gccgattgag aatccgtgcc ccgaaaaccg cattcacaat gttcgaagca
     8221 tcattacggc caacctcaat acagtcccag acgagcagtg ccaatcccaa aaaaacgaga
     8281 attgtaatca aatccacggg caaactctcc gattttgccg atcgttcctg cctgggggtc
     8341 aaaactctag tggaaggccc caaaagacaa ccattcgaac cagcaccccg ccaagaatcc
     8401 gtcatttgcc aaggcggaaa gtcacttccc acaaaatgtc caatttaccg gagagcgaaa
     8461 ctcggcaaaa gaaagagatc ctcgaaatca ctggccgaat gatgccgcag taatgagaat
     8521 aaagaggagt tatgcctgcg tcacgcggga aacataaaac caaccgctgg ttaaaaaagg
     8581 cgcactgaac aacgaggacg tcaactattt cttgggaatt ttcagctatg gatcgccagc
     8641 aggtgcgaac aacgtgggaa aaggcctgcg atgaattgat ccgccgccgc gaatccgcag
     8701 gatactggcg gggatgtctt tgttcctcgc cgctggcaac cgcgacggcc attagtgcgc
     8761 ttgctgtgta ttccaggtcc cttgaaagca aaggtgggtc tttttccccc acaatggcgg
     8821 aatccattca atccggccta aggtatctcc ttgctaccca gcggccagat gggggctggg
     8881 gtgataccga gcggtcgctc agcaatatcg ccaccacata cctcgtccgc gccgcaattg
     8941 agcttggcgg aaaggcgatg gtgccggagg acgccctacg tcgtgctgac cagtacattg
     9001 agcgtcagaa ctcagaaaac ggcctaaggc agcgatatgg tcgtgatcag acatttgtgg
     9061 cccctatctt aaccaatgtg gccctggcaa aattgtgttc gtggaggagc gtaccccagc
     9121 tcccctttga attagcggcc tttccccaat ccctttatcg ttttctgggg ctgccggtgg
     9181 tcagttacgc cgtgcccgcc cttgtcgccg ttgggttggc gagatttcac ctcggaccaa
     9241 aggggaacac tgtcgtggct tgcctgcgac gggcggtcac accaaaatgc cttgcagtgg
     9301 tcgaaactat gcagccccaa agtggcgggt ttctcgaagc cgttcccctg accgcctttg
     9361 tcaccatgtc gctcagtgcg atcggtttgg ccggtcactc tattgtccag aaggccgttc
     9421 gctttctcct gtctttgcag cggagcgacg gaagctggcc cgtggatgtt gaccttgcta
     9481 cctgggttac cacactagcc accaatgcca ttgcagcagg ggaatatccg ggctggatga
     9541 atttggtttc cttggattgg attttgggtt gtcaacaccg cgattttcat ccgtttactg
     9601 gtgcagaccc cggtggctgg ggctggaccg atcattccgg cagtgtcccc gatgctgatg
     9661 atacgtcggg cgcgctactc gcactggcaa atttctggca gaagggagat gctgagacac
     9721 ggcgcaagat actaccctcg gctttgttgg gattagactg gctcgttcga cttcaaaaca
     9781 aagacggcgg ttggcctacg ttttgccggg gatggggaaa gttaccattc gatcgaagtg
     9841 ccgtggactt gaccgcgcat gctgtgcgag cgttccacac atggtacaat cttttgatgg
     9901 gtcaacctcc cgttgacctc gccggagggc gtatcgtttt cgacgagctc tggccagatg
     9961 ccgccgcgcg aagactgggc cgcctggaaa acatcgatcg cgcactcttg ccgcgtctga
    10021 aaagaagcat cagtaaaggc gtggcctttc ttaggcaaaa gcaggaacat gatggccgtt
    10081 ggttaccctt gtggtttggg aacgaacgtc tgcccaacga ggaaaatccg gtctacggaa
    10141 caagtcgctg tctgatggct tacgtcgccc ttaaccaaca gcacaaccca gcggctgaaa
    10201 aggctatcgc gtggctgttg caggcccaaa accgggatgg aagttgggga agatctgcaa
    10261 gcgaagaacc gccaaggcgc gacgacgaga caagcccaga tgccccagga tcgatcgagg
    10321 agacatcgct ggccatctcg gcactggcca gtttcagaga caatcctgca gtgcccgcgg
    10381 cgctggagcg gggattgagt tggctgactt ggaacattga aaacggcaat tggcgtatcg
    10441 cgacgcccat cggcctttat ttcgcacggc tgtggtatta cgaagatttg taccccattg
    10501 tgttttccgt cgaggcactc gggagatacc tccggcattg cgatcaagaa gagaaaacgt
    10561 gaatatgagc tgccctgctt ttacaatagc cattgaggcc gttcaaccac attgattctg
    10621 tgcaggtggc tacgcaatca tgggcgaaga gctggtacgc agtcaagaaa cgacagtaca
    10681 ggacctgggt tcaggcgagt ccatcaaagt ggtgccccag gatcgctcct gccgggaaca
    10741 gattcgccgg gaggccatcc gccttgcgga acagttgaat cactctgagc ttctcacccg
    10801 ggataagatc gaattgacgg ccaagatagt tctccaaaac cttgggttgc ctcaatctta
    10861 cctgggctgg acgatggtgg cggtgggatc ggccttctgg cggtcacaag tgatgcgggt
    10921 tccgtatcat cgccgcctct tgcttttgcc ccattgcatg cgaaacctaa gtttgtgtgc
    10981 aggcagctat aacgctgagg gtttacagtg cgcatcgtgc ggtgggtgtc cgctgggttg
    11041 gcttcgtgat gtggctttgt ccctcgggta tcgcgtgctg attgccgaag gttcaccaat
    11101 tgtgctgaga ctcatcctct cgggtgaagc cgatgcttta ctgggcgttg cctgcctcaa
    11161 ttccctggaa cggtcgctgg agcgggtctt gatggcaggc gtaccgagca tggcagttcc
    11221 cctcattgtc ggcggttgtc gcgaaacagt cgctgatgag gactggatct tggagatgat
    11281 tgaaacccct tacatcgatg ccgagtcttc agggcgatcc tatttgcctc tcatgcgatt
    11341 ggccgcacag cttgtcgaaa gtgagctcga tggtcttgca ccgcgaatct tccccaccac
    11401 cgagcaacgg gaggacgcca aggtcttgtc gatcaccgaa gacattgcct atcagtacgt
    11461 taaagatggg ggaaagtatt accgtccgtt tatcgtgttg agcgtctatg atgccttgag
    11521 cggtggaaag cacgctcatc gaaaccatgg cgacgccgcc accacgatgc ccacggcggt
    11581 tcgtcgcgtt gccctcgctg tggaggtatt tcacaaagca tccacgattc acgatgacat
    11641 tgaggacgac gattcctatc ggtacgggcg tcccacgtta cacaagcgat ggggcattcc
    11701 cgtggccatt aatgtgggtg attatctgat cgggcttggg tacgcgcttc tcgcagagac
    11761 gcaagatgaa atgggaaaag atgtcgtggc cgacctcgtc accctctttg cccgtgccca
    11821 caggaatctt tgtactggtc aaggctcaga gttgtggcta gcccaaaggc ccgagctagc
    11881 cttcacggtg ccgcaagtct tgcggattta ttccttgaaa acggggacag cgtttgaggt
    11941 tgctctcttg gcgggtatgc ggttggccgg tgaaatgcca agctggtgga aattggtgcg
    12001 tcaatttgct cgggcatttg ggatcgcttt tcagattcaa aacgactgtc aagactggca
    12061 aggccaggag cataataaac tccgtcacgg gaccgacgcc gtagcacttc ggcctaccat
    12121 attctccgca ttggcatggc acacaacacc gggcgagttc gcagcgttaa tcgagaagtg
    12181 tcaatcaaaa agagaagcaa aacacgagga tattgccagg ctgttccaag agttcttccg
    12241 caagcatggg cttttcaata cggtgcagga gatgctttac ctggagcgca cgcgggcact
    12301 cgaaatagcc gataagattc cgcacgagaa tcttcgcatc ttcttgcgtt atttgacaga
    12361 gtcagccatc agacccacgc tcgagtgaac gccgacacgt tcgtttcccc aagttgtcct
    12421 tccatatagt tttgctgacg gtgttgatca attctatcgg gcaatagtgt ctttggatgg
    12481 acaaccggat ttgaggtgga atttgaggaa gggcagacat ttatgggacg ttttttgaga
    12541 gccgcataag ctaaaacgat ttgctgggaa cccatgcgca atagcatcga tttgttcttc
    12601 catttcgtga cccgttccgc ccatttgtcc gaggaaatgc ggcttaggca ccgccagtgg
    12661 ctagttggcc agcaacgacc ggatggggga tttgcagggc gagaaggtga cagcaatctg
    12721 tattacacgg ggttcggggc acgattgtgc gcgattcttt ggtcggatgt gaccgatcct
    12781 tgggtatcca tgacccaata tgttgcgatg cagttagaag cgattgattc ggcggttgat
    12841 ttggcatcgg cagccacgat ctacgccttg gttcgcctcc ttgttgacga atcactcgcc
    12901 caatcacttg aggatcgact gcgggctcgg gccgaacttc tttttgcccg tttctatcga
    12961 ccggacggtg gatttgccaa atcggagcaa agcggctttg ggagcaccta cgcgactttc
    13021 ttggccttga acaccatgaa cctcttgatg cttccgcttc ccgatcggga acaaattgtg
    13081 cagttcgttt gctcccgaca acggcctgac ggtggtttcg ctgaggttcc ccacgcgaag
    13141 aacgcagcca ccaatcctac tgcggcagcc atctcgatat tggccgacct cgaagcactc
    13201 gacaaaatcg acttgcagca agtgatcgaa tacctgtttc gcatgcagcg tgctgacggc
    13261 ggtttttccg ccgggcccca tgcgccggtc agtgacctgc tgagtacgtg cgccgcgttg
    13321 agcgcgttgt ggctgctcga ccgctccgaa tttcgtcgcc ttgacgtgga gcgagtcgag
    13381 aggtttgtcc aaagccttga aaacgctaca ggcggcttca ggggatgtcg tcttgacgac
    13441 caatccgatg tggaatacac gttttacgga ctagccaccc gtgccttgct tcattcctga
    13501 cgcaacacgg gtacgctcaa aggtggtctt gcttttccac ctgtctttac caaagagtca
    13561 tttagtttcc agaggcggta acagttccgg gacagcctcc tcggtgtcca tgtggtaagg
    13621 caaccggtaa acgcggctgc catcgcggga gcagaaatac aggcacgaag gagaaaacgt
    13681 gtcgggattg ccgtcggccc aaaaaccgta gaaatcctca tgagcgttta cgggccgccg
    13741 cacgtagttg tgattgaagg cactgccgcg tgtgacgtca cgcacctttt gccagctctt
    13801 acccttgtca tcgctcttcc agatagcgat ttcgccacct gccccgattg gttgcggccc
    13861 gcgctcggtt gggccaatga cccgccagac gtcgccctca atataaaggg ctcccgtgct
    13921 gtaattgtgg tttccctgcg taatctccga gaactcccag ttcgttccat tccaatgtgc
    13981 aatggtccag tagcgcgggt caccttctgg cccaggctta taacttgctg cagtcgtgta
    14041 aagaatcacg ggatatccct gactgtccag atcgacgtcg tggatataaa ctaggcggcc
    14101 ttccttttca tactcccgca caagggccgg attgattggg tcggataagg gcaaagtcaa
    14161 cacttcccct tgaatatttg tccacgtcgc accgacgtcc tttgtttcga tgtaatacaa
    14221 attagttcgg cgatccacat tgccacccgg atgatagttg aaagccgtaa aaacgcgctg
    14281 gtcttttcgc ccacttgttt gataatgccc acccatgccc gcaaatttga tttcttctga
    14341 ccactggaat ccgtcgggac ttgtggaaaa gtacaattcc cgtcctttcg tgtacttggt
    14401 gaaaaggtag agaaatcccc gatcaggcaa ccaccacggt tgcggataag tcatttcgcc
    14461 ttctttaatc agttcgaaat gttcaatgga atatggcttc tcacttcgat aaataaagcc
    14521 gggacgtgag cgagcacgac cactgataaa tacccataga tagccctgtg ggtcaattgc
    14581 gagagccgca ttgtcgtgag gatcatcgac accgttcttg tcgtgaacga tcgtcggccg
    14641 tggaacgact cccctcgtat gatcgaaata gccaaccatg ttcagcaagt gccgcttgcc
    14701 ctccttggcc ccgccataga cgaaaaacgt cttgttgacc tccttgcaat aaatcgcgat
    14761 cggcacatgg ttggccgtgt aggtccccag accgccggag tatttgtcgc cgaattccgt
    14821 ccactggccc aacgtgaacc aaatcccgcg atacccgtta tcgcggtcag aagcaatcac
    14881 gtcagcacaa ataaaggcca cgcacaggaa ccaaatcatt agcctggcca gcatattaca
    14941 cgttttgttc atcgaaacct ccaggttaca gtcctaacaa tgaggcaaaa gaggcaaacc
    15001 cgcgcccaca ctcgtgcaga caacaacgcc gcacgtgtca atcatcgctc ccacccctga
    15061 caacctcgcg actttcatag ggtacccgat tggaaaggaa aatcaactcg cacttcggca
    15121 atcactgacg agccgcactc gagttaggaa ttacacgcaa aaaagcggtc agttttggtg
    15181 ttttcggtta aaatcttcac tgcgagaaac cttgccgcac gatttccgct agtccgcttc
    15241 gatagtcggg atagcgaagc gacatgccga agcctttgac caacaactgg ttggagaccc
    15301 gcttggtcac cgggctatgg cgccgagccg cacttgagac ctggtctgtt tcaaaggcag
    15361 gaagggactc agcaaaccgt ggggaggcaa cttgtagcaa tcgagacaat tcgttgtaga
    15421 agtcccgtcg gctaaccggg aagccatcca cgacattgac gatgcacggg ggctggccac
    15481 ggctgatgac aagatcaatc gcagtagcag catcttcgac atggatgagg tttagcatgg
    15541 catcagggga agtcggcaaa ggttgtccct gaaccaattg ggaaacgagc ggaatccgcc
    15601 ctggtccata aaccccgcca agtcgaaaaa tgtaggcccg atcgccccac gacgattcta
    15661 gcagtaagtc ttccgcagca gccaacgcgc gggcagagtc cctttgggga tttcttggtg
    15721 tctgctcatc aatccaccct tcgggatgat tgccatacac gcccgttgag ctgaccaagc
    15781 caatctgaga ggttgtgtga cgaaacctgg tcaggatgtt atctaagccc tctttgtaaa
    15841 gcctccaacg tggaacgtcg cggtgcccag ttggcccgac cgagtaaagg atcacgtcac
    15901 cgcctggcaa tctctcgagc gaagttggat cgagaacgtc tgcgacaact acctcaaagc
    15961 cttgggcctc aaggggcgca accctctcgg aggagcttgt caccgcacac acgcaatagc
    16021 cttttttcag ccaaagccgt cctacgtagc tgccaacata accacaccca acaattacgg
    16081 ctttgtgcat aacgtttcaa ccaacgaagg agcgaaaatt aacgttttta caggaatgcg
    16141 tggcgcaggt atcacaggaa agaacgcgcc tcatccagaa caattgcgat tctctcgtcg
    16201 ttcagggcca cgccgtttaa accaccgaag ggccgtttcc cacgatacgt tgtgacgttg
    16261 agcaaacgtc atacggattc cccatcatcc agcggtgaag gagaccacga ttgaggtggg
    16321 ccgctcgatg gcttgtaggt cgcaaggtat gccgaaagaa tgatctgcgc ggagacctta
    16381 tccagacgtt ctcgacgccg tttgtggctg aggtgagccg atcgcaacca cgtgtccgcc
    16441 gtctgagtgg aaaaccgctc gtccatgaaa acgacaggcg ttccggtaac cgcttccaac
    16501 cagtgtccga attcacgggc ggcttgcgaa ctttcacttt ctgacccgtc gcaatgtacc
    16561 ggcaatccga caacgaaaag gacgatctcc tcctgcttaa ccaacttgcg aaaatactcc
    16621 gcgtcttgct gcgggccacg tcgctgatag accgcccaag gtgtggctag cacccgacca
    16681 gggtcgctta aagcgatccc aatacgggtc ttaccgaagt cgatacccgc gatgcgccct
    16741 ttcgcaggaa tctccggcgt gctcggtaac tggggatgct catcctcaaa tgaacgcgcc
    16801 attgttgatt aattcttttg gcacttggca ctgtcatcgc gctgtcagat gggcccaggc
    16861 cctgaagaat tcgcgccctc acatgcctgg ggcagccggt cgtttttcct ggacccaaac
    16921 gtagtactcg tacagcttga gcttggaggc agcttcatcg tccaggtacg caatggcatc
    16981 gcggtgtaac tgaagggcac tcgcggtcac catgcttgtt accggtccct cgatggctgc
    17041 agccactgcg tcagccttgg cgtaaccgtg agccagcaag atgatacgct tcgcctccaa
    17101 gattgttccc acacccatgg tgatggcgta gatgggaagc tcttcgcctt cgtcgaatag
    17161 gtccttgatc tcttccagcc gacgccgcga cagggtctta atccgcgttc gtgagcccaa
    17221 cgagcttcca ggctcattaa aggcgatatg accatcggtg ccgatcccca ggatttggag
    17281 gtcaatccca ccgcactctt tgattcgttg ttcgtaccac cggcaaaacg cctggtagtt
    17341 ctgcgatgtg cccgacggca tatagatatt ctgcggtgca atattgacgt atttgaaaaa
    17401 gtgctcatgc atgaagtaat gctgactctg aggatgttcc ttccgcaggc caacgtattc
    17461 atcaaggtta aatgtgacca cctgggaaaa atctatttcg ccctcgcgaa acatgcgcac
    17521 caattcacgg tacagcccca caagcgtgtt gcccgtagcc aatcccagca cggcgttggg
    17581 cttcgaatta aggacctggg caaccgcctg ggccgcggcc tgacataact cctcgtaact
    17641 cttcttgatg atgacttcca tcgtttccct ctgcgaggtt cgccacctgt ttttgtacgt
    17701 caagacgcta cgcgatatag acgtccgtaa ccggtgcagc gacaggaaat ggaccggaac
    17761 gcaatgaccc tcttgaaatt accgttaaga aggtcacact caataacttg agtgtcggcc
    17821 taatggggac cgctgtcaag ttctgccaat ggcctgggcc acggtgggac aaaacagctc
    17881 ggggcacgaa aaccgtgcct taggaatttg gttgatgtgc gttagcaatc ccgtcttgag
    17941 ggagtatgag atgaccatga gcaactccac aatctctacc caacgcttgg cccttgttac
    18001 ggggggatca cgcgggatcg gtgcggccat ctgcacgaag ttgggggaat tgggcttcca
    18061 tgtctttgtg aattacgtcc acaatcagga ggcggcaaat aaggtcgctc aggatatctg
    18121 cgagaaaggc ggtacagcga cagtcctgcc tggggatgtt ggctccgaat cagatcgccg
    18181 tcggatgttg agtgtcatcc gtgaatatca acggctcgat gttttggtga acaacgcggg
    18241 gatcacctcg gtaggacggc gtgatatact cgaagcgact gaagaaagtt gggaaaaggt
    18301 tttcaatacg aacctcaagg gtcctttttt cctcacccaa gccattgcca acgaaatgat
    18361 ccgttggatt cgtgagggcc gacagccaca gggttatatc atcaatgtgt cttcaatttc
    18421 tgcctttgct gtatccacca atcgggcgga ctattgtttg acaaaagcgg cattggcgat
    18481 gatgacgcaa ctcttcgccc agcggcttgc ggacgaaaaa atccaggtct ttgaagtctg
    18541 ccccggtgtc atcgccagcg atatgacggc ccctgtcaaa gaaaaatacg accggctcat
    18601 cgccgaaggg gcctggccca ttcgcagatg gggagaaccg gaggacgtgg ccctagcggt
    18661 tgccgcttta gtccagggtt atttcccctt cagcacgggc aaccggtttc ttgtggatgg
    18721 aggctttcac attcggacgt tgtgattgcc gccaacatta tttggaatcg gactggccga
    18781 taaacgggaa taactggcga atctcttgcg cactgggctg ccgcccatat tcacaaatct
    18841 caaaggcctc ggcatacttt acggcccgtc ctttttcagg tccatacagt tcaatgagtc
    18901 tatcccgata ctgatcggca attctcgcga acctctgccg gaaggactcg cggacttgtt
    18961 cccgcttttt ctgccgttcc tcctccgtct tgggcgccgc ttcggctgat cccagggcac
    19021 ctccttcaac gaactgggat tcaataacca ccaaggcatc gagcttttga tcgatcacct
    19081 cgtcaatcgc taccacaatg tcggcacgga atggattggg acgctgaaaa ctgtcgtacg
    19141 agaacaaaaa cacagggttt cttttcagtg gtgggacatc tggacagaag aacggcacgg
    19201 ccaccatgta ggcacagtct tgcatcaaga cacccacgta acggtggtca ggatggtaat
    19261 cgttgggccg atggccgatc acaatatcgg catcccatgc ccgaatcagt ctggtcatga
    19321 tgcggcgatt ctccaaagtc ggcaacaact cgccgtcatg aatatccagc acctccgtgg
    19381 tgattcccag aatcttggcg gcctcctgta cctcgcgcat ccgccgctga gcaagcgcgc
    19441 cgcctgcgat cccccaatgt ccgatatcgc cattggtaac tgacacaaat ttgaccttgt
    19501 acccgtgtcg agcccaaagg gcagccgtac cgcccgcgcg gatttcacaa tcgtccggat
    19561 gggcgccgaa aacgatgatc cgcaagggac gatcctgagc ccaagtgagc gaatgtccac
    19621 ccgaactcaa tagccaacaa aagcccagga ccaccaggca acgccccacc aaggaccgca
    19681 tcgcaccaat cctgctgcaa aagtgaaacc gtagcatgac ccatctcctt taccttgaat
    19741 ccactttcca gaaaatgact cgctacaccg tacaacaacg tccccgttag aaacgggaaa
    19801 tcacagccaa acatgttgta ataaggggag ggtcacttcg caagtcacaa gtgcatgata
    19861 ctgggcacac cagccttccg gcactttttc atcttccgac tgcctttgcc gtgaacgtta
    19921 taatgacgtg aagggtgttc cgataagaaa tcgggaccca catgttgcta agggtcgtcg
    19981 gagatttgtc agaaagtcat atgagcgttt cggcaatcga actgtggtgg caaagcgcag
    20041 gtccgccaac cgtagtcttc caaacgatgg gccgaatatg gcaactgggc ctggtcaatg
    20101 cttggcgtga cttgcagcag gcgggccacc agttgccttc cttgccgcgt tttctcgtgg
    20161 ctcacaatcg cagccgtgtg gagaacatat tgaaagaacg cccggcgagt tttgtcatcc
    20221 ttacgatgcg acatgagcat gccaaatcgt ttctcgtcaa tcttatgtcg ctcaaagacc
    20281 gtttccctac agcgggtttt gcggtggtcg cgccgagggg acacgttctc gagggcattt
    20341 gtcgggaata cggtgcgcat tgcgtggtgt cgtcgcctcg agagctcaaa aaggtggcga
    20401 tctggctcgc ctggcatctg cgacggttac cacctatttt gaacaatcca cgggctcaga
    20461 tttgggaccg acttccttgg tcgcagtggg attcaaggaa aaatatggcg tcttcaagtt
    20521 gactgcgcac acatgagata atccccacgg gtaactagcc tcaccaaaac tatctctcag
    20581 aaaatgcgta ggatcagtag ctcaggaaag gtaccatcac tcccactcac tctcatccgc
    20641 gggagttgcc cctcctgggc ttcgctgggc aatacttggt ggaaagcgtg ccgttctgtt
    20701 gcattttccg ggaagaacag caaaagcccg gcgtggtgag cggtgctctg tgaacgggac
    20761 atactcggtg gatgagacgg taattcaaac taacaaggag aaatataaat catgacaaaa
    20821 aacccaataa cccgcgagga aatcctcagg ggcctcgatg gctttcagga ccccgaattg
    20881 ggccgtagca tcgttcgttt ggatcaggtg caggacgtac aaatctccga cggtttggtc
    20941 aaaatcgtct tgggactcac gtcattttct gcccctctat ggccggaatt gcgggaggaa
    21001 gttcgtaacc tgttgcgaac gcaattgacc ccggacgtcg actgggaagt ggtgattagg
    21061 gaatttcccc ggccggccga gaagttgggc acgatcggcc taacggccaa gtatgtgatc
    21121 gcggtaggat cgggtaaggg aggcgtcgga aaaagcacga ttgccgcggc gcttgccttg
    21181 ggcctggatc gggcgggttg taaagtgggt ctactggatg ccgacgttta tggaccaagc
    21241 attccccatc tccttgggtc agatgaacgc cccaccatgt acgagggtaa aatcattccc
    21301 attaccgtgg gcaatctccg cgttatgtcg atgggcttta tcgttcctaa ggatgaggcc
    21361 gttatttggc gaggccctat gctccatggg gcagtgcaac agttcttgcg agataccgac
    21421 tggggtgatt tggactatct cgtggtggat atgccaccgg ggacgggtga cgtggcgatt
    21481 agcctctccc aattgctccc ggtttccggt gccgttgttg tttgtacacc ccaggagctt
    21541 gcacttctcg atgcagtcaa ggccattacg atgtttcgca aggtcaacat cgacgtgctg
    21601 ggcattgttg agaatatgag ctactttatc tgtcccaatt gtggtacgcg gcacgacatc
    21661 tttagttctg gaggggccag aaagaaggca gatgagcttg gcctgccgtt tttgggcgag
    21721 gtccctctca atgtgatgct tcggattaag ggagacgagg gacgccttgt ggaatgcctc
    21781 gccgaagaaa atattggccc ttatttcgac gcaatttgca agaacgttgt gcggcaaatc
    21841 gtcaaccaac gtcgcggcac gccaccgttg ccttccctct ccgtgctgtg agtttcaaga
    21901 tgcttaag
//
LOCUS       DAOSUT010000064        21644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1586_length_21644_cov_10.865348, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000064 DAOSUT010000000
VERSION     DAOSUT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_21644_cov_10.865348"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="PLD05_07605"
     CDS             complement(<1..1904)
                     /locus_tag="PLD05_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOP77341.1"
                     /translation="MAQGRGEDLATALEESINRAIQYLKKQQRDDGSWPEPINMPCGM
                     TGLCTLALLSAGVSADDPTIAKALRYLRRFSPSDIDKTYSITLQTMVFCRAEPARDAP
                     RILENIRWLERTQIKDGPDRGGWSYPGSSADNSNSQFALLALYEAERVGVRASDQTWT
                     FAKEYWEGTQNPDGSWGYRRGQAGTGSMTCAGIASLVIIRDIAYQPTATVDANNRINC
                     CGSSPQSVDRVQRGLAWLERHFTVRQNPQAGSSWLLYYLYGLERVGRLSAQRFIGPYD
                     WYREGSAFLLELKSTVAQGGIPTDYWVGPGLENDPLLATSFALLFLAKGRRPVLVTKL
                     KYGNAENWNRHRNDVNNLAAYTESRWQMDLGWQVTDLMAASVEDLLQSPVLYLGGAES
                     PLPNEAEQQQRLAQKIRDYVDRGGFVFAEAVCPGGGFDEGFRRLIAMAFPEPEYALQM
                     LPPEHPVWRAEEPIPSQYLRPLLGVEFGCRTSVIYAPPTAVSPSQPRPSLSCLWELSR
                     AGRGVTFAPAVQAEITAGLNLGLNVLAYATNRQLKYKYAFFNQQSSRAVRDNVRRNKI
                     AVAELLHPGGCHVAPRALNNLLEAAADQLRLRVELSKNEIAISDPALFDFHLAFMHGR
                     NAFRLTDEER"
     gene            2441..3442
                     /locus_tag="PLD05_07610"
     CDS             2441..3442
                     /locus_tag="PLD05_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOP77342.1"
                     /translation="MLEELQKVIVGQTEVIEQLLAAIFTRGHCLLVGVPGLAKTLMVN
                     TLAKILDVQFKRIQFTPDLMPSDITGTNVLEEDEHGKRYFRFVEGPVFTNILLADEIN
                     RTPPKTQAALLQAMQEREVTVGQQTYPLPEPFLTIATQNPIEQEGTYPLPEAQLDRFM
                     FNIKVDYPTLEEEERILAATTRSEKVEVRKILSGRAILNLQKLVNSVAVSPYIVQYVA
                     RLVRATRPRDESAPQFVREFIDWGAGPRAGQFLIQGGKALAAMDGRFAVSIEDVRKLA
                     IPVLRHRISTNFQAQAEGLTSDDIILRLVAEVPAPAIPKFEQPAPPQLNPDILRPLT"
     gene            3692..4588
                     /locus_tag="PLD05_07615"
     CDS             3692..4588
                     /locus_tag="PLD05_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP77343.1"
                     /translation="MTNVQKYLKPEVARQIKRLDLRAQFIVKGFLHGLHASPFQGFSV
                     EFSEHRRYVPGDDPMDIDWLVYAKTDRYYVKKFEAETNITGYLVVDASRSMGYTYRQE
                     TTKFDYAICLAAALCYLMIHQQDPVGLWVFDEAIRHSLAPKSRRGHLGTVLSVLAKLE
                     PVGNTDIARCLAQIASILRRASLIILFSDLLTDPEPVLQGLHRLKHRGHDIIVFHILD
                     EAEVSFPFQGTVELEEPESGERLEVDAAAYRADYLEAVQQFREYYRKECFRAGIDYVP
                     LDTSMAFDKALMEYLLRRRARF"
     gene            4626..>4918
                     /locus_tag="PLD05_07620"
     CDS             4626..>4918
                     /locus_tag="PLD05_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOP77344.1"
                     /translation="MGFVTPMLFWAGLGLATIPVVLHLIMRQKARRVVFPALRLVKQR
                     YEVRRRRLQLQHVLLMLLRMLLILLVGVGLARPKLEWGGPFAGEEAPVAAVLI"
     assembly_gap    4919..5018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5019..6686
                     /locus_tag="PLD05_07625"
     CDS             <5019..6686
                     /locus_tag="PLD05_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77345.1"
                     /translation="HREIYIFTDLATAGWPSSEFGGLRERLLKTKNIAFYLLDVGIEE
                     PRETGIREVKLSEDVNIEGIPLEFQVECRRWGPAETRTVALYLEDDNSASATGAKQPV
                     KRAQASVSIPANGLAVTTIRLAGLTSGVHQGYVQLEGDSPLVHNDRWYFTVHVRSALP
                     VLIAAPSPAERRAVYLSQALAPEMLRRAGVARFQCDVIDLSALASKDLAKYQAVFVLD
                     PSLLPNQTWERLKAFVENGGGLAIFLGRSLGNPESWNTPPVSDLLPGHVLVQSRAPSG
                     DVYVRPTSYDHPVLKPFKALAGSVPWDLFPVYRYWVIDPLAENALVVLPYSDGRPCMI
                     ERVVGKGRVITVTTPVSDLPDSFPWNLLPMGQAWPFLILSNQLAFYLAGTGEIRTNYL
                     AGETVRVVIDSGGKLTGALLDLPPDALNATRSEPAFPIRLSFQPDKRDILITATERVG
                     HYRLRGGGKDEEFVFGFSVNYPGALMELNRVSKGELAELFKNVSFQIAHDRSDLSRQV
                     TAGRTGFDATPYVLMAMVLILGLEQVVANRFYRAAGQEPTKQRAAAT"
     gene            6791..9049
                     /locus_tag="PLD05_07630"
     CDS             6791..9049
                     /locus_tag="PLD05_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOP77346.1"
                     /translation="MELTLTFYPIAESYFLVAGLILTLLLLWRLFPLSQQLSGGRRRI
                     LLALRVATIIVVLFVLLRPTLVYLTRTKQAASVVLLIDQSQSMSVPDEVGGKTRFDAV
                     VETLQKNAAVLQKLAEDVQIHSYAFDGQLHALPLEKGLPKLPDAPRGNQTAIGAVLED
                     VIREVAGQRVIGVIMMSDGAQRAIYPRDTPPQPAAQRLRSLDQPLYTIRFGRARAGAE
                     GRDIVLEDLFAPDRVFVKNELNIGTYLRAIGYANRTIEVQLFFETEPGKPEMVEKQRV
                     TVETSDERIPVRFRYIPEATGERKVSIVVPPQPGEALITNNEQSTLVKVLAGGIKVLY
                     VEGALRVEQRFLRKALDASPDIHVDYVRLELRSREGRPADMDRRLQPGGYDVYFLGDV
                     DATLFEGQELASLADAVSKGAGLLMLGGLQSFGAGGYAETPLAEVLPIRMNRFERQQP
                     NEPVREDLHITGPVRMVPTPVGLKHFVMTLSPSPAENQRLWESIPPLDGANRWAGIKP
                     GAIVLAEDDRGGPLLVAQFYGQGRVVAFAGDSTWRWCLHGFETAHKRFWRQLTLWLAK
                     KEDTQQNPVWIHLAQRQIPLGERLFFEVGAGDPLGEAITDVTWQVEIVSPSGKRHPVA
                     VRQQGKNWQGEFAETAEPGDYQLEVQASQNGQVLGQDKARFLVVKRDVEMDQPASDPG
                     LLAALASASGGRSCAPEEFPEVLAELRQRLKEMEVPVATRVTLWDRWPVLLIFVGLTS
                     LEWFLRKRWGLV"
     gene            9100..10284
                     /locus_tag="PLD05_07635"
     CDS             9100..10284
                     /locus_tag="PLD05_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77347.1"
                     /translation="MRDTCYPGLRVAVVGRLAAMSRQELQKHLSERGLFLISDLSDNP
                     DWVVFGEYDLAWADDPRIASLLDKPSPPEVIPESELWRRLGLTPDCGNTKSLFTPAMI
                     ADLVKVPVREVRRWYRLGLLTAVQVVHRLPYFDYQGLLDARRLAELWESGIPSQRLNR
                     VLENVCRAIPRWKKRLPQLSFVVHGETILLQSGDKLIDYRGQLQLPLVFEDDGPSKKD
                     CETVLRIADHIAQRQEDSSLSLAEIMEMAEWMESQGNWEKAAEAYRVALAVGGPNAEV
                     CFRLADMLYQMGDLNGARERYYMAVELDEDYIEARASLGSILVELGQTELAIAAYQGA
                     LAIYPDYADAHFLLAKLLDDLGRHEEALEHWRRFLALAPESPWASHAQGRLVLNDETP
                     NP"
     gene            complement(10432..11298)
                     /locus_tag="PLD05_07640"
     CDS             complement(10432..11298)
                     /locus_tag="PLD05_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOP77348.1"
                     /translation="MNVIDFFWIFLMVSALQPVLRQKMIEAARQRVIARIERKRRSRV
                     IVLIHRQETMSLLGFPLMRFIDINDSEEVLRAIQLTDPDIPIDLVLHTPGGLVLAALQ
                     IARAVKMHPAKVTVFVPHYAMSGGTLIALAADEIVMCKHAVLGPVDPQINQQPAASIL
                     RVLQRKNPDEIDDQTLILADIAEKAINQVRMAVSELLAGKMPQEKAEEVARLLCEGTW
                     THDYPLTPETAQQLGLPVSTDMPVEFVQLLQLYPQPRRGQPTVEYIPTPYRYQQPKAY
                     PKTSSLFGRELG"
     gene            complement(11324..12061)
                     /locus_tag="PLD05_07645"
     CDS             complement(11324..12061)
                     /locus_tag="PLD05_07645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOP77349.1"
                     /translation="MIPIRDTIPSRNVPLTTYFLIFANVLIFLFQLELPEESLQRFFY
                     YFGIVPARYTHPAWAEYVGLPVDNYWPFVTSMFLHGGWMHLIGNMWTLWIFGDNVEDR
                     MRPLRFLLFYLCCGIAAGIVHFLTNPNSTIPTIGASGAISGVMGAYFLLFPHSRVITF
                     IPILFYPVFIALPAVVYLGIWFLTQFLSGVGSLLPGQQVGGIAWWAHIGGFVAGMVLC
                     PLFVERQRPCRGCYPDEYGTELGWMRW"
     gene            complement(12172..13614)
                     /locus_tag="PLD05_07650"
     CDS             complement(12172..13614)
                     /locus_tag="PLD05_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77350.1"
                     /translation="MPTQWGAFLATASLAEQTPLPEETPKKETRLEGPRDVLAAFGVT
                     ESFLQSFEDGQPVQPAERELLLRVLFYSHMFQPPEIAEWAESPPCWSDVHNELDGLRG
                     KLFRVPGYVKKAMAEDVPPELSSRLGMKSYYLCEVAGDDGISYWVYAKRLPRKWKLDE
                     PIHEKTLVHGIFLKLASENPESPQPILVTDRPGWFPDNLLGRLGMDCALFDDVVPTRI
                     NERFVEASSPATDDTESLLDRLRLTGRDRECFYQLMAAANRTRPGELFRIAKEELARQ
                     QRTASSVVPLFNDPAEQQGKLVLLYGTARRIEEVTVENPDIQKRLGISRYYTIYLFTE
                     DSQNYPVVFCVIHLPPGVTPGEGPRYAVDLALAGFFYKTWAFRPQRNLSPDAPPHTWQ
                     LAPLLIGREALKVTSRAAQNTGVMTFTLLGGVLILIMVVVIVATWFTWREKKRPPFLA
                     SSQISAGGEQPAPDFEKMAGPEDRVPNDRE"
     gene            complement(13779..14639)
                     /locus_tag="PLD05_07655"
     CDS             complement(13779..14639)
                     /locus_tag="PLD05_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77351.1"
                     /translation="MLRFDGYRRAPSLPSQREQRRLLLLVLLIGGVILIPQWLISWRL
                     FAPDTFLNNERHNSRSRVAQPETLSRSQDDSRFAGRSEELLKQLSEIDFSAVEDNTPF
                     GKKETTAWFSTLHLLTQFSVDDLRSANPPWVTFSQLYRNPSNYRRKLVALRGEFRGIA
                     YYPAPANDKGIDGYYQAWLQPERENDPVVVYCLQLPPGFPEGMRIQEPVEVIGVFFKR
                     WVYQAQDGLRVAPVVLTKEPMWKRTPISTTGSLTSPQNILLAVAISVVLTVILLAYLF
                     RPQQRHIPTS"
     gene            complement(14766..>16839)
                     /locus_tag="PLD05_07660"
     CDS             complement(14766..>16839)
                     /locus_tag="PLD05_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77352.1"
                     /translation="VVLPPGCENLESTTVELLERFLKLGGHVLVCGESPSLVNATPSQ
                     RIDSLKRLPGWKKCDPTDLPLILSERQDPRIRIIREKDDRGLLFHQRRRLADGEIVVL
                     VNTSDTESTRGVIETSYAGAEIWDLEEGNTRPAHFVRKDNRLLLPFDLSPVGSLVVFF
                     SQKETVSGPAQEFQEQPVSLSPISIARLQPNVLTLDYVDIQVGSELRRGIYYYQACDW
                     VFKKHGLPGNPWDRAVQFKDEIIRQSFPPETGFTLTYRFQIRESVPSDLAIVIERPDL
                     YAITCNGQPVSWNGKDWWLDRAFGKIDISRSVRVGENEVQLVARPFTLFHEIAPAYVL
                     GDFSLEPAEKGFVIVPAKPLQIASGLGAHTAQPNHTMWLSAGIGFDRSAAPADDPQPW
                     LVFDFGRTVSLAVIQIWNYNEVNLTSRGVKDLEIVLLDDAPSLEAGKVIARVTLPQAL
                     GRETVSGTSGLPATIAIPETKVRRLGFRILSNHAGVTFPADKPAVDNAFVGLSEVRFL
                     SKKDNALEEIKGVQVACSSELKGNFQRLAQHLIDGSGLGQTGWNNQGCPFYGHGVGYT
                     GHFQWKKGEGQYLVRLGRWEGSLAKVILNGRDCGVIFKPPYECDIASALKEGDNTVTV
                     VVIGTLKNTLGPHHAGVMRGSAWPHAFAQGPAEGPPAGDRYDTIAYGLFEPFRVVHRV
                     PSSNFGAR"
     assembly_gap    16840..16939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16940..18283)
                     /locus_tag="PLD05_07665"
     CDS             complement(<16940..18283)
                     /locus_tag="PLD05_07665"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP77353.1"
                     /translation="MKNGSFCVAVGLIVVLSHVGVTDCADEPLTTSTAVKSLLAKPPR
                     QFSSAPLWVWNDLLTDEQIVSTLHDLAGQDVKQVFVHPRPGLITPYLSDEWFRLWKLA
                     LKTAESLDMNVWIYDENSYPSGFAGGWVPEVMPESIGRGLAVKETGNLAEVPPSAVAV
                     YRLTNDGYENVTQQFRAKEQLGGNQYLYAEVVRSAPSPWHGGRPYVDLIYPGVTQKFL
                     EVTLDAYQREVGEWFGSRIPGSFTDEPRLLAAGQLSWTDDLADRFWETYGYDLLDHLP
                     SLIRPVGDWKRVRHDYYRLLLHLFIERWAKPYYQWCERHNLEFTGHYWEHEWPQCRFV
                     PDNMALYAWHQRPAIDCLMNQYAEHTHAQFGNIRAVRELSSVADQLGRQRTLCELYGA
                     GGWDLRFQDMKRIADWLGVLGVNTFDEHLSFISIRGARKMDHPQSFSYHEPWWDSY"
     gene            complement(18358..21192)
                     /locus_tag="PLD05_07670"
     CDS             complement(18358..21192)
                     /locus_tag="PLD05_07670"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOP77354.1"
                     /translation="MKIAVASPTLSVHYFVVISAGLMMTPLNYGHAHANEQSSAVVGR
                     QFVFESAPFKSCHASTIAETEAGLAVAWFGGDREGAENVGIWLSRLTQDGWSAPVEIA
                     TGVQEDGRRYPCWNPVLFQPKEGPLVLFYKVGPRPSSWWGMMMSSQDGGRTWGPPKRP
                     PDGFLGPIKNKPIELADGTWLCPSSTETPESPSRWQIHFEFTRDRGVTWEKRIPPQGA
                     EPLDAIQPSILRLSNDRLVAVGRTRQRRVFFTASYDNGQTWTPVQKTTVPNPNAGIDA
                     VTLRDGRQLLVYNHATTGRSPLNVALSHDGVNWVPVLVLEDQPGEFSYPSVIQTRDGL
                     VHIVYTWKRQRICHVIIDPEKLTLEHQEPTSQPQAGHGSAPRKEKTPNVIIVITDDQG
                     LGDLSCQGNPILWTPHLDRLAAEGVRLTDFHVAPVCTPTRGQLMTGQDALRNGARSVP
                     AGTNMIWRHVPTMAEIFRASGYRTGHFGKWHLGDHYPDRPTDRGFEKSVWFGGWGVSS
                     DVEFDNDCLKIRYRDGDQVSVADRYCTDFWFDEALRWMEECRKSQQPFFCYIATNAPH
                     GPLWAQDKDAAPYRSRVPAAVANFFGMIANIDENMGRLENWLIDSGLREDTIVIFMTD
                     NGGTAGRKVFNAGLREGKGSYYEGGHRVPCFIRWPNGGIQGPRFISQPTQVQDILPTL
                     VDLCGLRVPPAARFDGLSLAEVLRNPQANLPDRMMVVQYGGRTRAEKNDCCVIWGNWR
                     LVKGSELYDVSRDLAQEQDLSQVHPDVVKKMQSFYDHWWTQIEPTVYEYQPVVVGTEQ
                     ENPVILSSNYWAGVDVDNHHRVSAGEGGPRGGPYHIDVATPGRYRIELRRWPFHCNEP
                     LGSAGPKQTVAGRSLEEVIRNRKALPIAGAVLVIGNQEFQKQTSPEAMGITFEVSLPA
                     GRTILQGWFLNSQGEDLCGAYYVSVEKLAN"
BASE COUNT         4913 a         5799 c         5976 g         4756 t
ORIGIN      
        1 tttcgctctt cgtcggtcaa acgaaaagca tttctcccat gcataaaggc caagtggaaa
       61 tcaaagagtg ccggatctga gatggcgatc tcattcttcg acagctccac acggaggcgg
      121 agttggtcgg ctgccgcttc cagaagatta ttcaacgccc gcggtgccac atggcaacct
      181 cccgggtgga gtaattccgc cacggcgatt ttgttgcggc gaacgttatc ccgtaccgcg
      241 cgggacgact gctggttgaa aaaggcgtat ttgtatttga gttgccgatt ggttgcgtag
      301 gccaggacgt taagccccag attcagcccc gcggtgattt ctgcctgaac ggccggcgca
      361 aaggtaacgc ctcggcctgc ccgcgacaac tcccagaggc aggatagcga aggcctgggc
      421 tggctggggg acactgctgt gggcggcgca tagatcacac tggtccggca gccgaattcc
      481 acgcccaaca atggccgaag gtattgcgag gggatcggtt cttcggcccg ccacaccgga
      541 tgctcaggcg gtagcatctg caaggcatat tcgggctcgg ggaacgccat cgctatgagt
      601 cggcgaaatc cctcgtcaaa gccgccacct gggcaaaccg cctcggcaaa aacaaaacca
      661 ccgcgatcaa catagtcgcg tattttttgg gccaaacgct gctgctgctc ggcctcgttg
      721 ggcagcgggc tttctgcacc tcccagataa aggactggcg actggaggag gtcctccacg
      781 cttgccgcca ttaggtccgt gacctgccac cctaggtcca tctgccagcg gctttcagtg
      841 tatgctgcga gattgtttac atcgtttcgg tggcggttcc aattttctgc attcccatat
      901 ttcagtttgg tgacgaggac aggacgtcgt cccttggcga ggaagagcaa ggcgaaagac
      961 gtggcaagga ggggatcgtt ttccagtccc gggccaaccc aatagtcagt cggaatacca
     1021 ccctgggcca cggttgactt caattccagg agaaaggcag agccttcacg ataccaatca
     1081 taaggcccaa tgaatcgttg cgcactcagg cgaccaacac gctccagacc ataaagatag
     1141 taaagcagcc aggaactccc tgcttgcgga ttttgcctaa ccgtgaaatg acgctccagc
     1201 catgccagcc ctctttggac gcgatccaca ctttggggag aagaaccaca acaattgatc
     1261 cgattgttgg catcgacggt ggccgtaggt tgataggcaa tatcccggat aatcaccagt
     1321 gaggcgatcc cggcgcatgt catactgcca gtgccggcct gaccgcggcg atagccccac
     1381 gatccatcag ggttttgggt gccctcccaa tactccttgg cgaaggtcca cgtctgatcg
     1441 gaagccctta cgccaacacg ttcggcttcg tacagggcta aaagggcaaa ctggctattc
     1501 gagttatcgg cgctgctccc gggatacgac cacccaccac ggtccgggcc gtccttgatc
     1561 tgggtccgtt caagccagcg gatgttctcc agaatccggg gggcgtcacg agcaggttct
     1621 gcccggcaaa agaccattgt ttgcagcgtt atagaatacg ttttgtcgat atcagagggc
     1681 gagaatcttc gcaaataccg cagggcctta gcgatcgttg gatcatcggc ggaaacacct
     1741 gcgctgagaa gggccaacgt gcacagcccg gtcatcccgc aaggcatgtt aatcggttct
     1801 ggccaagagc catcatcgcg ctgctgtttt ttcaagtatt gaatcgcccg gttgatggat
     1861 tcttccaagg ccgttgcaag atcttcgcct cgcccctgcg ccatggctac ccccgggcta
     1921 attgtgaaac gcgggcatga tggcatccag gtcggtgccg caaaacaaag gagtaaccag
     1981 gtcacgatcg caagtaatgg gcaagtcagt tttctgacca ttcggtcacg ttacgaaaaa
     2041 tgtcaacggc ggtttcaata aaaacgacaa agcaattcgc gttctcggtt ttctgcgcca
     2101 gaaaaacatc gacgccacac cggctctttc attttggtag gtcggcgtgc acccgagcaa
     2161 cggtaccgga agaatttcag gatttggcag gtacgtaagg agcacaaggt gttcggttga
     2221 ggcaaaccgt aacggtccaa agaattgacc tttctcatca tctatctcgt cccttccctt
     2281 tggcatctta taatgaagca tggatgaggg aaaagagatt ccctgctcca taggaaaagc
     2341 gcgaagtcgt ggcggtcttt cttgctgagg tgaaccattg tccacaaaac cccgcagtct
     2401 tggtgacgtc cttcaggaat tcagccacta ccgccggctg atgctcgagg aactccagaa
     2461 ggtgattgtt ggccagacgg aggtgatcga gcaactcttg gcggctattt tcacgcgcgg
     2521 tcactgtctt ttggtggggg tgcctggttt ggcaaaaacc ctcatggtca acacactggc
     2581 caagattctg gacgtccaat tcaaacggat tcagtttacg ccggatttaa tgccctcgga
     2641 catcactggg acgaatgtct tggaggaaga tgagcacggc aagcggtatt ttcgctttgt
     2701 ggaaggcccg gtgtttacca acatcctgct tgcggacgag atcaaccgta ccccaccgaa
     2761 aacgcaggcc gccttgctgc aagcgatgca agagagggag gtgaccgtcg gtcaacagac
     2821 ttaccctctg ccggaaccgt ttttgaccat cgccacccaa aacccgatcg agcaggaagg
     2881 gacctacccc cttcccgagg cacaactcga tcgttttatg ttcaacatta aggttgacta
     2941 tccaacgttg gaggaggaag agaggatttt ggccgcaaca acgcgcagtg agaaggttga
     3001 ggttaggaag atattatccg gcagggcgat cctcaatctt cagaagttgg tcaattcggt
     3061 tgcggtaagt ccttacattg tccaatacgt tgcgcggtta gtgcgggcca cacgtccccg
     3121 cgatgagtcg gcgccacagt ttgttcgtga gtttattgac tggggagcgg ggccgcgggc
     3181 gggtcagttt cttattcagg ggggaaaagc cctcgccgcg atggatggtc gctttgcggt
     3241 ttccattgag gatgtgcgaa aattggcaat tccggttttg cggcatcgga tcagcaccaa
     3301 ttttcaggcc caggcagaag gactgacaag cgatgacatt attcttcgtc tggtcgctga
     3361 ggtgcccgcc ccagccattc ccaagtttga acagccggct ccgccgcaac tcaacccgga
     3421 tatcttgcgg cctttgactt agagtccatc gccaaaagtc tccctctccc taggggagag
     3481 ggttgggatg agggcaagcg aacgtcgcag ttgattgaga aaaccggcag caattcgagc
     3541 caccctcacc cccgacccct ctcccgccca atgggagagg ggagcttatg ggataggctc
     3601 tttgagggcg acgggctggt cgctgaaacg gtgggatagc attaggggct aattcccgcc
     3661 tggataagat cttggggctt gtggcgaaat tatgacgaac gttcagaaat atcttaaacc
     3721 agaggttgca cgccagatca aacggctcga cttgcgggcc cagttcatag ttaagggatt
     3781 tttgcacggt cttcacgcca gcccgtttca ggggttctct gttgagttta gcgagcatcg
     3841 acgttatgtc cccggtgacg atccgatgga catcgattgg ttggtctacg ccaagaccga
     3901 ccgctactat gtgaagaaat tcgaggccga gaccaacatc acgggttatc tggtcgtcga
     3961 tgccagccgt tcgatgggtt acacctaccg ccaggaaacg accaaattcg attatgcgat
     4021 ttgcctggcc gcagctttgt gctatctcat gattcaccag caggacccgg ttggcctgtg
     4081 ggtctttgac gaggccatta gacattccct tgcccccaag tcgcgtcgcg gacatttggg
     4141 aacagttctt tccgtattgg caaaactgga acccgtggga aacacagata ttgctcgatg
     4201 ccttgcgcag attgcctcaa ttttgcgtcg ggcaagtctg atcatccttt tttccgatct
     4261 tttaaccgac cctgagcccg tccttcaggg tttgcaccga ctaaaacaca gagggcatga
     4321 catcattgtg tttcatattc tggacgaggc tgaggtgtct tttcccttcc aagggacggt
     4381 cgaactcgag gagcccgaaa gtggcgaacg cctggaagta gatgcggcgg cctatcgggc
     4441 agattatctg gaagcggtgc aacagtttcg tgagtactac cggaaggagt gttttcgtgc
     4501 cggcatcgat tatgtgccgc tcgatacgag tatggccttc gacaaggccc tcatggagta
     4561 tcttctgcga cggcgagcaa gattctgacc tttgacatgg catgcacgaa taaagcgttt
     4621 gtgagatggg atttgtaaca ccgatgcttt tttgggcagg attagggcta gcgacgattc
     4681 ccgtcgtgct ccatctcatt atgcgccaaa aggcccgtcg cgtggttttt cctgcccttc
     4741 ggctggtcaa acagcggtac gaagtgcgac gacgccggtt gcagctccag cacgtcctcc
     4801 tcatgttgtt gcggatgctg cttattcttc tggttggtgt tggactggcg cggccaaaac
     4861 tggaatgggg gggcccattt gccggtgaag aggcacccgt ggccgcggtc ttgatcttnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tcgggagatt tacatcttca
     5041 cggatttagc cacggccggg tggccaagtt ccgaattcgg cggcctacgg gagcgcctac
     5101 tcaagacgaa aaacatcgcc ttctatcttc tggacgttgg gatcgaagag cctcgggaaa
     5161 cgggcattcg ggaggtgaaa ttgtcggagg acgtcaacat tgaagggatc cctctcgagt
     5221 tccaggtgga atgccgtcgc tggggacccg ccgaaacccg aactgtagcc ttgtacttgg
     5281 aggatgacaa ttcggcgtca gcaaccgggg cgaagcagcc cgtcaagcgg gcacaggctt
     5341 ctgtttcaat cccggccaat ggtttggcgg taacaacaat caggttggcc ggacttacat
     5401 cgggtgttca tcagggatat gtgcaactgg agggtgactc gcccctggta cacaacgatc
     5461 ggtggtattt tacggtacat gtccgctctg ctcttcctgt attgatagcg gccccgtcgc
     5521 cagccgagcg tcgtgccgtg tatctttcgc aggcgttggc cccggagatg cttcgccggg
     5581 ccggggttgc acgctttcaa tgcgatgtga ttgacctgag cgctctggcg agcaaagatt
     5641 tggccaagta ccaagccgtg ttcgtacttg atcctagcct cttacctaat caaacctggg
     5701 aacgactgaa ggcctttgtc gaaaacggcg ggggccttgc gattttcctc ggtcggtcgt
     5761 tgggcaatcc cgagtcgtgg aacacaccgc cggtttcgga ccttttgcca ggccacgttc
     5821 tcgttcagtc tcgcgcacca agtggggatg tctatgttcg gcccacaagt tatgaccacc
     5881 ccgtgctgaa gccgttcaag gccctggccg gttcggtccc gtgggacctt tttccggttt
     5941 atcgttattg ggtgatcgat ccccttgcag agaatgcctt ggttgtcctg ccttacagcg
     6001 atggaaggcc atgcatgatc gaacgtgtcg tgggcaaggg ccgggtcatc acggtgacca
     6061 cgccggtctc ggacctgcct gattcatttc cgtggaatct cctgccgatg ggtcaagctt
     6121 ggccgtttct cattcttagt aaccaactgg ccttctattt ggccggaacc ggtgaaatac
     6181 gaactaatta tttggctggt gaaaccgttc gtgtggtcat cgatagcggc ggaaaactga
     6241 ccggggcgct tcttgatctt ccgccggacg cactcaacgc aacgcggtca gaaccagcgt
     6301 ttcccattcg cttgagtttt caaccagaca aaagagatat cttgatcaca gctaccgaac
     6361 gcgtcggcca ctatcgtctt cgcggagggg gcaaagatga ggaatttgtc ttcgggttta
     6421 gcgtcaatta ccccggggcg ttgatggaac tcaatcgggt ctccaaagga gaactcgccg
     6481 aactctttaa gaacgtttcg ttccagatag cccatgatcg ctcggacttg agccgacagg
     6541 tgaccgctgg gcgaacaggc tttgatgcaa caccctatgt cctgatggcg atggttctca
     6601 ttttgggact ggaacaggtc gtggcgaacc ggttttatcg cgctgcgggg caagagccga
     6661 ccaaacagag ggcagcggca acatgaggtg gttcaaaggg gaacgtgcgt gaacagcaag
     6721 tgtgtggggg catgaacagg gtagtacttt atttgggaac ggccacagaa cggcgttatt
     6781 gattatcggt gtggaactaa cactaacgtt ttatccaatt gccgaaagtt atttcctggt
     6841 agccggcctc attttgacgc tgttgctact gtggaggctt tttccactca gtcagcaact
     6901 gtcaggggga cggcgcagga tactccttgc gctgcgtgtt gccaccatta tcgtggtctt
     6961 atttgtactg cttcggccga ccttggtcta tctgacaagg acaaagcaag ccgccagtgt
     7021 cgttcttctc attgaccaat cgcagagcat gtcggttccg gatgaggtgg gggggaagac
     7081 gcggtttgat gccgtcgtgg agacgctcca gaagaacgcc gccgttctcc aaaaacttgc
     7141 ggaggatgtt caaatccaca gctatgcatt cgatgggcag ttacatgccc ttccgttgga
     7201 gaaaggcctc cctaagttgc cggacgcgcc gcgtggcaat caaacggcca tcggggctgt
     7261 gctggaagat gtgatacgcg aagtagccgg gcagcgggtt atcggagtca tcatgatgag
     7321 tgatggggcg cagcgagcga tctatccgcg ggacacacca ccgcagcccg ccgcacagcg
     7381 attgcggtcg cttgatcagc cgctctacac aatccgcttt ggacgggcgc gcgcgggggc
     7441 cgaaggccgc gatatcgtcc tggaagatct gttcgccccg gatcgtgtct ttgtgaaaaa
     7501 cgagttgaat atcggtacat atcttcgggc tatcggttac gccaatcgca caattgaggt
     7561 gcaacttttc tttgaaacgg aaccgggtaa acccgaaatg gtggaaaagc agagggttac
     7621 tgtcgaaacc tctgacgaac gcatccctgt ccggtttcgt tatatccccg aagcaactgg
     7681 cgagcgaaag gtaagtattg tcgtcccacc gcaacccggt gaggctttga ttaccaataa
     7741 tgagcaaagc acactggtca aagtcctcgc agggggaatc aaggtcttat atgtcgaagg
     7801 tgcccttcgc gtagaacaac ggttcctccg gaaagctcta gatgcctcgc ccgatattca
     7861 cgtggactat gtgcgactgg agcttcgcag ccgggaggga cgcccggccg acatggatcg
     7921 ccgtttgcaa ccggggggat atgacgttta ctttttgggc gacgtggatg caaccttgtt
     7981 tgaagggcag gaattggcgt cgctggcaga tgcggtttca aaaggggctg gccttttgat
     8041 gttgggagga ttgcagagtt ttggtgccgg gggatatgca gaaacgccgc tggcggaggt
     8101 cctacccatc cgaatgaacc gtttcgaacg gcaacaacct aacgaacccg ttcgagaaga
     8161 ccttcacatc acaggacctg tgcggatggt ccccacgcct gttgggctga aacattttgt
     8221 catgacgttg tcgccttctc ccgcggaaaa tcaacgactg tgggaatcga ttccaccact
     8281 ggacggggcc aatcgttggg caggcatcaa acccggagcc atcgttttgg cggaggacga
     8341 ccgtggtgga ccgctccttg ttgcccaatt ctatggacag ggacgcgtgg tagcttttgc
     8401 gggcgactcc acatggcgat ggtgtctcca cgggtttgag acggcccaca aaaggttctg
     8461 gcggcagtta accctatggt tggccaagaa agaggacacg cagcagaacc ccgtttggat
     8521 tcacctcgcc cagcgccaaa ttcctctcgg cgaacgccta ttctttgaag tgggggctgg
     8581 cgacccgctc ggagaagcga tcaccgatgt gacctggcaa gtagaaatcg tttcgccaag
     8641 tggtaagcgt catcctgttg ccgtccgcca gcaaggcaag aattggcaag gcgaatttgc
     8701 agagacagcc gagccgggag attatcaact ggaagtgcag gcctctcaaa atgggcaggt
     8761 gctcggccag gataaagcgc ggtttttggt cgtcaagcgt gacgtggaga tggatcagcc
     8821 ggcatctgac cccggtttgt tagccgctct ggcttcggct tcaggaggcc gatcatgcgc
     8881 acccgaagag tttccagaag ttttggcgga acttcggcag aggttaaagg aaatggaagt
     8941 ccccgtagcc actcgggtga ccctttggga tcgttggccg gtcttgctca tctttgtcgg
     9001 tttgaccagc ctagaatggt ttctccgaaa gcgctggggt ttggtgtaat tggcgggacg
     9061 gttacctgac ttgtgatgtt ggaaatggaa attcccagaa tgagggacac atgttatccg
     9121 ggactgcgtg tggccgttgt ggggcggctg gcggcgatgt cccggcaaga gttgcagaag
     9181 catttgtcag agcgaggact ttttttgatc tctgatcttt ccgacaaccc tgattgggtt
     9241 gtgtttgggg agtatgacct ggcctgggca gacgacccgc ggatcgcatc acttttggat
     9301 aagccctctc cgccggaagt gatccctgaa tcggagctct ggaggcgttt gggattgaca
     9361 cctgattgtg ggaatacgaa gtccttgttc acgccggcta tgattgccga cttggtaaag
     9421 gtccccgtca gggaggtgcg gcgttggtat cgcctgggat tactgacggc ggtgcaagtc
     9481 gtccatcgtc tgccatactt tgactatcag ggccttcttg atgcccgacg attggccgaa
     9541 ctgtgggagt cggggatccc gtcccaacgg ttgaataggg tcctcgaaaa tgtctgtcgt
     9601 gcgatcccac gttggaaaaa acgccttccg caactctctt ttgtggtgca cggcgagacg
     9661 attcttctcc agtctggaga caaactgatt gattaccgag gccaactcca gttacctctg
     9721 gtttttgaag atgatggtcc aagcaagaag gattgcgaga ccgtcttgag gattgccgat
     9781 catatcgcgc agcggcaaga ggattcgtcg ctttcgttgg ctgagatcat ggagatggcc
     9841 gaatggatgg aatcgcaagg aaactgggaa aaggcggccg aggcctaccg cgtggccctg
     9901 gccgtcggtg gcccaaatgc tgaagtgtgc ttccgcctgg ccgatatgct ttatcaaatg
     9961 ggagacctca acggcgcacg ggaacgctat tatatggccg tggagttaga tgaagactac
    10021 atcgaagccc gggcaagctt gggatccatc ctagttgagc tcggtcaaac agagttggcc
    10081 atcgctgcct accaaggggc cctggctatc tatcctgatt atgccgatgc ccacttcctt
    10141 ctcgctaagt tgctcgatga tcttggccgc catgaggaag ccctcgaaca ctggcgccgg
    10201 tttttggcat tagcccccga aagcccgtgg gcgtcacacg cccaaggtcg ccttgtcttg
    10261 aacgatgaga cgcccaatcc atgattaaag cgcgaggttg tgagacggcc tcgactgact
    10321 aggagcctat cctacaacct cccctctccc gtttggcggg agaggggccg ggggtgaggg
    10381 tgactcgaat cgctgctggt tttctcgatc gactccgacg gtcacttgcc ctcaccccaa
    10441 ctctctccca aagagagagg aagttttggg ataggcttta ggttgctgat accggtaggg
    10501 agtagggatg tattccaccg tcggctggcc gcggcggggc tgtgggtaaa gttgcaaaag
    10561 ctgcacgaac tcgactggca tgtccgtact gactggtaat cccagttgct gagccgtttc
    10621 gggcgtgagg ggatagtcgt gggtccaagt tccttcgcac agcaagcgag ccacttcctc
    10681 ggccttttct tgtggcatct tgccggcaag cagttccgac acggccatgc ggacctgatt
    10741 gatcgccttt tccgcaatat cggcaaggat cagagtttga tcgtcaatct cgtccggatt
    10801 tttccgctga aggacccgca gaatggatgc tgccggttgc tgattgatct gtggatccac
    10861 aggtcccaac acggcatgct tgcacatcac gatttcatcg gcggccaagg cgatcagggt
    10921 cccacccgac atggcgtaat gggggacgaa aacggtaact ttggcggggt gcatttttac
    10981 cgcccgggct atttgcaagg cagccaagac caatccaccg ggcgtatgaa ggacgagatc
    11041 gatagggata tccggatcgg tgagttggat ggcacgcaag acctcctcgg aatcattaat
    11101 atcgatgaat cgcatcaggg ggaaaccgag gagactcatg gtctcttggc ggtgaatcaa
    11161 tacgataacc cgggaacggc gtttcctttc gatgcgggcg atcacccgct ggcgtgctgc
    11221 ctcgatcatc ttttgccgca gaactggctg aagtgccgag accatcaaga agatccagaa
    11281 aaagtcgatg acgttcattc cgctagccct ttcccttcaa ggcttaccac cgcatccaac
    11341 ccaattcggt gccgtactca tctgggtaac aaccccgaca aggtcgctgg cgctcgacga
    11401 atagaggaca gaggaccatt cccgccacaa atcccccaat atgggcccac cacgcaatcc
    11461 ctcccacctg ttgtccggga agaagactcc ccacaccgct aagaaactgc gtgagaaacc
    11521 aaataccaag atagaccact gccggcaggg caatgaagac gggataaaag agaatcggga
    11581 taaacgtgat cacccgtgaa tggggaaaaa gcagaaagta agctcccatg acaccggaaa
    11641 tcgcccccga ggcaccaatg gtggggatcg tggaattggg attcgtcaaa aaatgcacaa
    11701 tgccagccgc gatcccacaa cataggtaga agagtaggaa gcgaagcggc ctcatgcgat
    11761 cttccacatt atccccaaaa atccagaggg tccacatatt cccgatgaga tgcatccagc
    11821 ctccgtgtaa aaacatgctg gtaacgaacg gccagtaatt gtccacgggc aaccccacgt
    11881 attctgccca ggccggatgt gtgtatcgag cgggtacaat cccaaaatag tagaagaacc
    11941 gctgcagcga ctcctctggc aattcaagtt gaaatagaaa aatgagcacg ttggcgaaaa
    12001 tgagaaaata ggttgtgagg ggaacatttc gactggggat tgtgtctcga atcggaatca
    12061 tagaagctgt gattcatcca aagagttcaa gttacccacg atcaaagttc attgtgtccc
    12121 tacgtaaccg gtgtgcaggc tgggaagcca tttgtgccat ggccccatga tctactcgcg
    12181 gtcattgggt accctgtcct ctggccctgc catcttttca aaatcgggag ctggttgctc
    12241 cccgcccgca gaaatctgac ttgatgcaag aaacggtggg cgtttcttct ctcgccaggt
    12301 gaaccaggtc gccacaataa ccacaaccat gataagaatc aacacacctc ccagcaaggt
    12361 gaaagtcatc acgccagtgt tctgggctgc ccgactggtg accttaagag cttcgcggcc
    12421 aataagcagt ggggccaatt gccaggtatg gggcggtgca tcgggcgaga gatttcgttg
    12481 cgggcgaaag gcccacgtct tatagaaaaa acccgcaagg gcaagatcca cggcataccg
    12541 aggcccttca cctggcgtca cacccggcgg taaatggatg acacaaaaaa cgaccgggta
    12601 attttgagaa tcttctgtaa aaagatagat ggtgtaatac ctgctaatcc ccaatcgctt
    12661 ttggatatcg ggattctcga cggtcacttc ttcgatgcgc cgtgccgtcc cgtagaggag
    12721 aacgagtttg ccttgctgct ctgcgggatc attaaaaagc ggcaccacag aggaggctgt
    12781 ccgctgttgt cgggccaact cttccttggc aatgcggaag agctccccag gtcgtgttcg
    12841 gttggccgcc gccatcaact gatagaaaca ctcccgatca cggcccgtta agcgtaaccg
    12901 atcgaggagg gactccgtgt cgtctgttgc gggtgacgac gcttcgacaa accgctcgtt
    12961 gatgcgtgtt gggacaacat catcgaaaag agcgcagtcc atgcccaatc ggcctagcag
    13021 attgtcagga aaccatcctg gccgatctgt gaccaggatc ggctgagggg attctggatt
    13081 ctccgaggcc agtttcagga agatgccatg aaccaaagtt ttctcatgaa taggctcgtc
    13141 gagtttccat tttcgaggca aacgcttggc atagacccaa tagctaattc catcgtcccc
    13201 ggcaacttcg cacagatagt aggacttcat ccccaagcga ctgctcaact cgggcggtac
    13261 gtcctcggcc atcgcctttt tgacgtaacc cggtacccga aagagctttc ctcgcagccc
    13321 gtccagttcg ttatgaacat ccgaccagca gggtggtgac tcggcccact cggcaatctc
    13381 cggcggctga aacatgtgcg aataaaagag cactcgcaaa agaagctccc tttctgctgg
    13441 ctgtacgggc tgcccatctt caaaactttg gagaaagctc tcggtaacac caaaggcggc
    13501 caaaacgtcc cgaggccctt ccaagcgtgt ttcctttttt ggtgtctcct cggggagagg
    13561 tgtttgctcc gccaaactgg ccgtcgcgag gaatgccccc cactgagtgg gcatcgcttg
    13621 ggccgccagg ccacacgttt cgcaacgcag ccaagatagg cccaataggc caagcagtac
    13681 gagccaacac ggtattgatc ttttcactaa aagagccttt ccataccggc aagctttcgg
    13741 agacatgttc ataaaattca tccgccaaga acttggggtt acgacgtggg aatatgtctt
    13801 tgttgaggtc taaaaagata agccaaaagg atgacggtca gaacgactga gatggcaacg
    13861 gccagaagga tattctgggg tgaagtcaaa gatcccgtcg ttgagatagg agtgcgtttc
    13921 cacatcggct cctttgtcag aacaactggc gccacccgca atccatcttg ggcctgataa
    13981 acccaacgct tgaaaaaaac gccgatcact tcgaccggtt cctggatacg cattccctca
    14041 ggaaaaccag ggggcaattg caggcagtaa accaccacag ggtcattttc ccgttccggt
    14101 tgcaaccagg cctgataata tccgtcgatc cccttgtcgt tagccggtgc cggatagtag
    14161 gcaatgcccc taaactcacc gcggagagcg acaagttttc ttcggtagtt tgagggattc
    14221 cgatagagtt gcgaaaacgt gacccatggt gggttcgcag accggagatc gtccacagaa
    14281 aattgggtga gaagatgcaa ggtagaaaac caggcggtgg tttctttttt gccaaagggt
    14341 gtgttatcct cgaccgcgga aaaatcgatc tcggacaact gcttgaggag ttcttcactc
    14401 cgtccagcga accgcgaatc atcctgggac cttgaaagag tttccggttg agcaacacgc
    14461 gatcgagaat tatgtcgttc gttgttcaag aaggtgtccg gagcgaaaag tctccaggaa
    14521 atcagccact gggggatcaa aataacccca ccgattagca gcaccaggag cagaagccgc
    14581 ctctgctccc gctgcgaagg gagagacggt gctcgccggt agccatcaaa gcgaagcacg
    14641 gtgaaaactc ctatcgaatc tgcaaaaatc gcccatgtgc cgggcacgcg cggcatagat
    14701 cagcttgtcc agggcatcct tccctcattc taggttactt ctcctagagg gcaaaagatg
    14761 tgaaattacc gtgctccgaa gttggaactt ggcacacgat gaaccactcg gaacggctcg
    14821 aataatccat acgcgatggt atcgtagcga tcgcccgcgg gtgggccttc ggcagggcct
    14881 tgagcaaaag cgtggggcca ggctgagccg cgcatgactc ccgcatgatg gggtcccaga
    14941 gtattcttga gcgtgccaat aacgacgact gtgacggtgt tatcaccctc tttcagtgcc
    15001 gacgcgatat cgcactcgta agggggttta aagataacgc cgcaatcccg gccattgagg
    15061 atcaccttgg ctagtgatcc ctcccagcga cccaatcgca caaggtattg gccctctcct
    15121 tttttccact gaaagtgccc tgtgtaaccg acgccgtggc catagaacgg gcatccctgg
    15181 ttattccagc cggtctggcc caatccactt ccatcgatga ggtgttgggc aagtctttgg
    15241 aaattcccct tcagttcgct tgagcacgca acctgaaccc ctttaatctc ctcgagcgcg
    15301 ttgtccttct tggacagaaa gcgaacttcg cttagcccca caaaggcgtt gtccaccgcg
    15361 ggtttgtctg cggggaatgt cacgcccgcg tgattggaga ggatccgaaa acctaagcgt
    15421 cgaaccttcg tttcgggaat cgcaatcgtg gcgggaagtc ccgatgtccc actgacggtt
    15481 tctcgcccca aggcttgcgg aagcgtgaca cgggcgatga ctttcccagc ctccagcgac
    15541 ggggcatcgt ccaaaagcac aatctccagg tctttcacgc cccgggatgt taaattgact
    15601 tcgttgtaat tccaaatctg aatgacggct aaagacacag tcctaccgaa atcgaaaacg
    15661 agccacggtt gaggatcatc ggccggcgca gcagagcggt caaagccaat tcccgccgat
    15721 agccacatcg tatgattagg ctgagctgtg tgcgccccta ggcctgaggc aatctgcagc
    15781 ggcttggcgg gcacgatcac gaaacctttc tcggctggct cgagagagaa atcccctaac
    15841 acgtaggccg gggctatctc gtggaaaagc gtgaatgggc gtgccacaag ctgcacttca
    15901 ttctcaccga ctcgtactga tcgggagatg tcgatctttc caaacgcacg atccagccac
    15961 caatccttac cattccacga gaccggttgc ccgttgcagg tgatggcgta aaggtctggg
    16021 cgttcaatca caatcgccag atcactgggg acactttcac gaatctgaaa gcgataggtc
    16081 aaggtaaacc cagtttctgg tgggaaggat tggcgaataa tctcgtcctt gaattggacg
    16141 gcccgatccc aaggattgcc cggcagcccg tgcttcttga agacccaatc acaagcctgg
    16201 tagtagtaga tgccgcgtcg aagttctgag ccgacctgga tatcgacata atcgagcgtc
    16261 aagacattcg gctgcaagcg cgcgatgcta atcggtgaca gcgacaccgg ttgttcttga
    16321 aattcttgag cgggaccgga taccgtttcc ttctgagaaa agaacaccac taggctaccg
    16381 acaggcgata aatcgaaggg gagtaagaga cggttatcct tccgcacgaa gtgagcagga
    16441 cgggtgttac cttcttccaa atcccaaatt tcggccccag catagctcgt ttcaataaca
    16501 ccacgggtgg attcggtatc acttgtattg accaaaacga caatctcgcc atcggccaag
    16561 cgccgccgct ggtgaaagag caacccccga tcgtcttttt cgcgaataat gcgaatccga
    16621 ggatcttgac gttccgaaag aatcagcggc aagtcagtgg ggtcacactt cttccaacct
    16681 gggagtcgtt ttagggaatc aattcgctgg gacggtgtgg cattgacaag ggaaggactt
    16741 tcgccgcaaa caagcacgtg gccgccaagt ttcagaaatc tttcgaggag ctccaccgtg
    16801 gtgctttcga ggttctcgca cccagggggt aaaaccaccn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnna taagaatccc accatggttc gtgatacgag aacgattggg
    16981 gatgatccat ctttcgcgcg cctcgaatgg agataaacga aagatgctcg tcgaaagtgt
    17041 tgacgcccag aacgcccagc caatcggcga tccgtttcat gtcttggaag cgtaagtccc
    17101 agcccccggc cccgtataac tcacagagtg tacgttgtcg tcctaactga tcggccacac
    17161 tcgaaagttc tcgaacagca cggatattgc caaattgggc atgcgtatgt tcggcgtatt
    17221 ggttcatcaa gcagtcaatc gccggacgct gatgccaggc ataaagggcc atattgtcgg
    17281 gcacaaaccg acactgtggc cattcgtgtt cccagtaatg gccggtgaat tcaagattgt
    17341 gccgctcgca ccactggtaa tacggcttgg cccagcgttc gatgaataaa tgaagcagta
    17401 accgatagta atcatgccgt acccgcttcc aatcgcccac cgggcgaatc aacgagggta
    17461 gatggtcaag aaggtcatag ccgtaggtct cccaaaagcg atccgccaaa tcgtccgtcc
    17521 atgaaagctg cccggcggca agcaatcgcg gttcatccgt gaatgaaccg ggaatccgtg
    17581 acccgaacca ctctcctact tcgcgctggt aagcgtccaa tgtgacttct aaaaactttt
    17641 gcgtgacgcc cgggtaaatc aagtccacat acggtcgtcc gccgtgccaa ggagatggtg
    17701 cggaacgaac tacctcggca tagagatact gattgccacc gagttgttcc ttggctcgga
    17761 attgttgggt gacattctca taaccgtcgt tcgtcaaacg atagacagca accgcggaag
    17821 gaggaacctc ggctagattg cctgtttctt taactgccag accccggcca attgattccg
    17881 gcataacttc aggtacccat cccccggcaa agcccgaagg ataggagttt tcatcataaa
    17941 tccagacatt catgtcgaga gactcggccg ttttaagtgc tagtttccac aagcggaacc
    18001 attcgtccga caaataaggc gtaatcaagc cgggccgagg atgaacaaac acctgtttga
    18061 cgtcttgccc cgccaaatca tgcagggtcg aaacgatctg ctcatcggtc aagaggtcat
    18121 tccataccca caaaggcgcc gagctaaatt gacggggcgg tttggccaaa agggacttaa
    18181 cggcagtgga tgtcgtcaaa ggctcgtcgg cacaatccgt caccccaacg tgtgatagga
    18241 ccacgatcaa tccaacagca acacaaaaac ttccattctt catacaagat tctcccattc
    18301 gcactaatgg tcaccaggca agcacatcga agatcacggt aagcgacatg gtgaccatca
    18361 attcgccagc ttttccacac tgacataata agccccgcaa aggtcttcgc cttggctatt
    18421 aagaaaccac ccctggagga tcgtccgccc agcgggcaag gatacctcga aggtaatgcc
    18481 catggcctcg ggtgaagttt gtttctgaaa ttcctgatta ccgatcacca acactgcacc
    18541 ggcgataggc agggcctttc ggttgcggat gacttcctcc agggaacgtc ccgccacggt
    18601 ttgctttggg cccgcggaac cgaggggttc attgcagtgg aacggccagc gacgcagttc
    18661 aattcggtaa cgtcccggtg tggccacatc gatgtgataa ggaccacccc ggggaccgcc
    18721 ttcacctgcg gaaacgcggt gatgattatc cacatcaact ccagcccaat agtttgatga
    18781 aaggatcact gggttctcct gctccgttcc cacaaccaca ggttggtact catacactgt
    18841 gggttcaatc tgggtccacc agtggtcgta aaatgactgc attttcttga caacgtcggg
    18901 gtgaacctgg ctgagatctt gctcttgagc caagtcacgc gacacgtcgt aaagttcgct
    18961 acccttgaca agtcgccaat tcccccagat gacgcagcag tcgttctttt ctgcccgagt
    19021 ccgcccacca tattggacga ccatcatgcg atcgggaaga tttgcctgag ggttccgcag
    19081 gacctcggcc aggcttagtc catcgaaccg cgccgcgggc ggcacacgta gtccgcacag
    19141 atccaccaaa gttggcagga tatcctggac ctgtgtcggc tgactgatga agcgagggcc
    19201 ttgaattcct ccgttaggcc aacggataaa gcaggggacg cgatgaccac cctcgtagta
    19261 actgcctttt ccttcccgca aacctgcgtt gaaaaccttg cgtcctgccg tcccaccatt
    19321 gtctgtcatg aagatcacga tggtgtcctc gcggagcccg gagtcgatga gccaattctc
    19381 gagccgcccc atgttctcgt cgatgttggc gatcatgcca aaaaagttgg ccactgcggc
    19441 aggcacgcgg ctgcgatacg gagccgcatc cttgtcttgg gcccagagcg gcccatgcgg
    19501 ggcatttgtg gcgatgtaac aaaagaaagg ttgttgtgat tttcggcact cctccatcca
    19561 gcgaagggcc tcatcgaacc aaaagtcggt gcaataacgg tcggccacgg aaacctgatc
    19621 accatcacga taccgaatct tcagacaatc gttgtcgaac tccacatcgg aagaaacacc
    19681 ccatcctccg aaccaaaccg atttttcgaa accacgatct gtaggccgat caggataatg
    19741 gtccccgagg tgccacttac cgaaatgtcc tgtccgatag ccgctggccc ggaatatctc
    19801 ggccattgtc ggcacgtgac gccaaatcat gttggtacca gcggggacac tacgggcacc
    19861 attgcgaagg gcatcctgac ccgtcatcag ttgcccccgc gtcggtgtgc acaccggagc
    19921 cacgtgaaaa tcggttaagc gaacaccctc agcggcaagc cggtcgaggt ggggagtcca
    19981 aagaatcggg ttcccctggc aagaaagatc gcctaacccc tgatcatcgg tgatgacgat
    20041 aatcacgttc ggtgttttct ctttacgggg ggccgatccg tggccagctt ggggctgcga
    20101 cgttggttct tgatgctcga gcgtcaactt ttcagggtcg atgatcacat ggcaaatgcg
    20161 ttgacgtttc caagtataaa cgatgtggac caaaccatcg cgggtctgaa tcaccgaagg
    20221 atagctaaat tcgccgggtt ggtcctccag cactaatact ggcacccagt tgactccatc
    20281 atgagacagc gcgacattaa gcggcgatcg ccctgttgtc gcgtggttat aaaccaaaag
    20341 ctgccgtcca tcgcgaagcg taacggcatc gatcccggcg ttaggatttg gcacggtggt
    20401 cttttggacg ggcgtccatg tctgaccatt gtcatacgaa gccgtgaaaa agacccgtcg
    20461 ctgccgtgtc ctccccacgg ctacaagacg atcgtttgat agtcgcaata tggaaggctg
    20521 aatggcatcc aggggttcag ctccctgagg ggggatgcgc ttttcccaag ttacgccccg
    20581 atctcgggtg aactcaaaat ggatttgcca acggctgggc gactccggcg tctctgtgct
    20641 ggaagggcat aaccatgtgc catcagccaa ctcgatgggc ttattcttaa tagggcccag
    20701 aaaaccgtcg ggcggtcgtt tcggcgggcc ccaggtacgt cctccgtcct gactactcat
    20761 catcatcccc caccaactgc taggccgagg accaaccttg taaaagagga cgagtggtcc
    20821 ctccttcggt tgaaacagaa cgggattcca gcaggggtag cggcgaccat cttcttgaac
    20881 accagttgca atttcgaccg gagcactcca gccatcttgt gtgagtcggc tcagccagat
    20941 gccgacattc tctgccccct cgcggtcccc gccaaaccag gcaacagcca acccagcctc
    21001 ggtttcggca atcgtggaag catggcagct cttaaacggt gccgactcga aaacgaactg
    21061 gcgaccgaca accgccgaag actgctcatt ggcgtgagca tggccgtagt ttaacggtgt
    21121 catcatcaac cccgcgctga tgactacgaa gtagtgaacg gacaacgtcg gcgacgccac
    21181 ggcaatcttc atgggtaaac ctccctcagc tcgtcttttt cggggtgccc cgcaagagct
    21241 ccaaatccag cggtacccac catgctatcg cttcgcgttg gggtcttcaa ttgtcgggaa
    21301 gagcaagcgc ggcggagcca gcgaaagaaa agaggtcgtc ccctggaaga cggattgcgg
    21361 gaaatgggtt cgtttgattg tgcgcgccat caaccggtcc gctggattgc gaaagaatca
    21421 cccgccctgc gtgtcgatga gctgatgcgt cgtacctgct cttcgatttt tttccgccgc
    21481 atcattgtct ttaaatcgtt tgtttaatac aattgtttgt ttgaaaggcc aggtagtgat
    21541 actgtgggag ggcccataat gtctgtggaa gtggatgaca cgaagtctcg gcttttgaca
    21601 gctgccgggg aactatttgc cgagcgaggg tttgaggggg ccac
//
LOCUS       DAOSUT010000065        21612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1591_length_21612_cov_9.491905, whole genome shotgun sequence.
ACCESSION   DAOSUT010000065 DAOSUT010000000
VERSION     DAOSUT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21612
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_21612_cov_9.491905"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLD05_07675"
     CDS             complement(<1..635)
                     /locus_tag="PLD05_07675"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOP77355.1"
                     /translation="MARWFRLFEKKRGNRRTGSEQIAGIWEGAFFGVLFLLGCVGFWL
                     TISLFVLPEWWVNRDFRPSPARILETRVGTTVRDGQTLYRPEVCIEYKVGGHTYRLWT
                     YDIHTVQGNGYSVDKERALKTLQRFKADDIITCWYDPRDPEIAVIDRGFWWWAWLVLL
                     IPLSFVILGAGGVSYHLWRWGKSVERLAVQGRPLSLSFSADPDAAMYPFVP"
     gene            complement(834..1250)
                     /locus_tag="PLD05_07680"
     CDS             complement(834..1250)
                     /locus_tag="PLD05_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOP77356.1"
                     /translation="MGSRVRLSPAALYEAELNKRLQMSTAEIGLSVRTTNCLEENGIL
                     TVAQLLNTSPERLLSIANFGEKTLEEVFLALERLGFFRRSRLRSLGLEDRPTPFVPEM
                     SESSVIQFRIVFDDSTATPVFEENNETEKVADSVGS"
     gene            complement(1675..2043)
                     /locus_tag="PLD05_07685"
     CDS             complement(1675..2043)
                     /locus_tag="PLD05_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOP77357.1"
                     /translation="MLLFKKKFLDDIRTGRKTQTIRVWKVRHMRAGQLSYIPGVGYIR
                     VDSVDEVALNDLTDEDAIPDGFLSVQELRAEIERLYSCDQLCAHRVFRIRFHVLPSDE
                     QRKIREQRRARREGTRRQDQ"
     gene            complement(2050..2658)
                     /locus_tag="PLD05_07690"
     CDS             complement(2050..2658)
                     /locus_tag="PLD05_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77358.1"
                     /translation="MGFHFFCPQGHILEAEIEQVGQAAVCPFCGVEMLIPAPLNQPTA
                     ASSQEDSRQASVNVPTESALLGHGQEDAIGGEVRGSPSFASVSGLSVEPGAFELSAGP
                     ERAIGVSLGAEESQSVFHIPCPNGHILETPADMLGRPVMCPICQTTFEPQYEASEEYR
                     RKREEMAAAAAARAARFWLQLAIGAAIIVLGGLIMLIVFAPR"
     gene            complement(2883..4349)
                     /locus_tag="PLD05_07695"
     CDS             complement(2883..4349)
                     /locus_tag="PLD05_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOP77359.1"
                     /translation="MRTRIAIYWLVVVFGFVGAAQQTGTVIFAEESSVRAKNVILMVA
                     DGAGFNTWLATSMYRGRVGREIYDQPGWVRLACTTFPLNTATQPKKTGQQDPNVVYSP
                     SKAWDAESGYKWLTSSATDSAAAATALSTGQKSYNNAINWSDMDQAMMGESLPEVAYR
                     AGKSAGVVTSVQWSHATPAALGGAHNRDRDNYAEIAQEMLAAPYLQVIMGAGHPLFDK
                     NGQPKKGKLEEADYRYVGGRQEWEKLTSGQHPGGWTLIERKEDFEKLTTGSAPKKVLG
                     TAQVATTLQQARGGLIKVVLPGEPFAEPLNDNVPSLAVMVEGALNVLDNDPDGFFLMV
                     EGGAVDWANHANQLHRMIEEQSDFLAAVEVVVNWVNKNSSWDETLVVLTADHECGCLW
                     GPDSDKKPFAPIQDAGAGKMPAARYNATKHTNSLVPLYARGVGAERFAAFVRGEDAQA
                     AQVWGISGKYVDNTDVGRILRSVILGQPVAVHHHVTMP"
     gene            complement(4489..5670)
                     /locus_tag="PLD05_07700"
     CDS             complement(4489..5670)
                     /locus_tag="PLD05_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOP77360.1"
                     /translation="MMRSIIKSVARWLVACAGQIDRRFVLILGMLMVSVLTVAPVSPL
                     QGLGAEPNPTEWRGVHIMAWGLAGGPEGLIPLKEAITKVFHPMGVNVIVFEVGYNFDY
                     RSHLELRQTKFITKKDAQDLVEHCRQYGIRVIPQFNCLGHQSWRNTLYPLLTQYREFE
                     EPPEAPPGQRWTELRSWCPLHPGVNGVVFSLIDELIEAFSADAFHVGMDEVLVIASKN
                     CERCRDKSPAEVFAKAVNDLHQHLVKERNLTMLMWGDRLLDAEMFPYGKGWETSRNGT
                     APAIDMIPKDIVICDWHYGKHQEYPSVRYFQEKGFRVWPAGWNNVDAVRSLIRYSRQN
                     NMGGVVGYLATTWVLEPGGFCWALVGERHPALVSQRALDAAQAFHAAMEELNASADQP
                     H"
     assembly_gap    5697..5796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5797..7448)
                     /locus_tag="PLD05_07705"
     CDS             complement(<5797..7448)
                     /locus_tag="PLD05_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOP77361.1"
                     /translation="MAQGTVDISSAELLVERVARLKAAVGRLCHLPAAAEMTVRLDRA
                     LQYLQAETVPAVVQAVLLGPTGAGKSTLFNSLIRRPGASPVSDAERCFTKTPYVAVSQ
                     ACRPLVAVPEELRPQFVESPLLGRLVLCDLPDIDGVLAEHWDIARQIVEQSHIVVYVT
                     SPEKRADFRVHEEVRRWAERKRWLFVLNRADEIPEGALEEVCRDWDRRLKELGFDPDE
                     RNRFVVSAKHPQRFQFDLLRRELIEHDYRQDLRQLRDEVFLAHVQYATSPTVLAPLQD
                     KIAQLRQAEERFAEEIRRVYQKGFQEPRVAAAFRAIVRQQVWQSLARRLWGIASLTAW
                     VHARWHYLMAGYRLLRLGVYGPSLWGIARATMAALRAFVTGIWPLKEVLEKLGSDTKW
                     RLKQLAADIHRTLEDHQLAEVLTSQVPDTAEPPSSSPATTLKTSLEQIVVGLLNEETD
                     QELLLELRNEVDEQAEELASRVAKWWVNVAANLLPTAVLVDIAVRLGWAWVGSAWYLN
                     LSRANYPPAGFYWLAMALLVLSVLPGHWLVSARTNGQINRLRP"
     gene            complement(7448..9364)
                     /locus_tag="PLD05_07710"
     CDS             complement(7448..9364)
                     /locus_tag="PLD05_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77362.1"
                     /translation="MIVGEDLPVRDPTYGIAEVHRCLAWLLSEIPQLKAALGELAEPP
                     GWEAMRKRFQSLEFCRNKGLSPPLVAVLFGPSGGGKSTWFRLLTGVNVPAGEEQRPTT
                     CACVVAVPKKLWEREFLEGVFPSCELAELQDPNQLRVSSDRVFFCPQEDPQGLPFILA
                     DVPDFNTVESQNWERAERMLARAELVLFLTYSESYSDRRTVEMLARCCRLAGQLVYVF
                     TKTEPEAAREKRRHLLQLVRERHDLGFDQKRVDGKTLSEFLAESLFYASPRVLSSEEL
                     SGERVIPLDETAPGFLSLLVGRDSHHLVVRGLIEAIREGIFYSKRLAEKAEKERNELE
                     GNLRTIEELIHRAALTVVEGMFPVGRLVQLAVEECEKRVPALIRKSLGWMNGVTNRIR
                     GKVMTVRDFFSLGGGSNREEETVDICDQSQLERKRLRQAVEHLVHEIRSHFPEEATAN
                     GIFSAERCEKARDVIESQPLPLAGIGWEASVREELAHWVRENKWTATTVSVIPTTLGI
                     SALGFLVADLGISGGLIGTLGTLSGLATVNGVAAWLLQYFGRWKLQQVAESAYQVWAE
                     YRAKELAQFLGEILEAELFKPWKERLSRLAKHDLGKTLRACETLQQVLVNVRDAREED
                     ATVFSSLGPAKETE"
     gene            10119..10829
                     /locus_tag="PLD05_07715"
     CDS             10119..10829
                     /locus_tag="PLD05_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77363.1"
                     /translation="MVPLQHVVCVRGGVARPLLALFFVGLFLINGFLFLIAIAFELAF
                     AILSLAFFTAAGSCLVYYFLRKKFFVLIRSEAGTEIAISFMPSVLEGQTVDFPKALMA
                     VEVLKNLALDALHRTPPTTVAPAFEETPAYPVEDQSQVSISPRVTPPPLTPEAVSPIF
                     TGPEPPPSHEPEPEEVFTPQAPEEVAMKALREAVKTYNNGQHEEGIYLLQEVVRLNPG
                     TKAARIAEKHLAKLGRHA"
     gene            complement(10918..12039)
                     /locus_tag="PLD05_07720"
     CDS             complement(10918..12039)
                     /locus_tag="PLD05_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77364.1"
                     /translation="MLERIRRLVIKEFLAIFRDPASRMVVIVPPLVQLLVFSFAATLE
                     VRNAGLGIYNEDVGVLGRELVARFEAIPVTFSRVISCSNDQEVREVLDSQQTLAIVHI
                     PQDFSRSLESGQIPVVQVLLDGRLSNAAMVVQAYIGRIVRQFAQEQQLNMPPRHSSVD
                     QIVPVTRVWFNENTSPMWSSVPALLAILTNLVALLVTSLSVARERELGTFDQLLVSPL
                     RPAEILIGKAVPAFVIAYLEGGLMVGAVIIVFGIPFRGSLVLLAMAMFVFLLAIVGIG
                     LWISSLAATQQQALLGSFTYMVPAVLLSGFATPVENIHPIIRWLSDIDPLRYMVAISR
                     MVFLEDPSLDVIVPYVAPLLPIAAFTLFSAAWLFRHRLE"
     gene            complement(12050..13210)
                     /locus_tag="PLD05_07725"
     CDS             complement(12050..13210)
                     /locus_tag="PLD05_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77365.1"
                     /translation="MNAVNRQNVRLSARRFRLRCVLALARKESYQIVRDPSSLVIAFL
                     LPVVLLFLFGWGISLDLGSIPIGLVVESPSRDAERLVASLRGSRYFIVREANHRQEME
                     PLLVGGRIKGLVIIPQDFAANLSRGSATVQILVNGSEANTAEIVRSYLENVLQTEHRH
                     RAEETGNRLIGDTIHLESRVWYNPNLDSRAALLPGSIAVIMTLIGTILTSLVVAREWE
                     RGTMEALLASSITRGEFLLGKFIPYFALGLTAMMLVALVSVIIFHIPFRGSWGLLIVV
                     SAIYLTVALGLGLFISTATRNQFVATQAALVVGYLPSFILSGLVFEINSMPTPIRLLT
                     HILPPRYFVSALRTLFLAGNVWEIVLQDTAILAVFATILWMLTIRKTRLTLE"
     gene            complement(13207..14973)
                     /locus_tag="PLD05_07730"
     CDS             complement(13207..14973)
                     /locus_tag="PLD05_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOP77366.1"
                     /translation="MIRFDHVTKRFSPKQRPALRDVTVEILAGAITGLVGPDGAGKTT
                     FLRLAAGLLMATEGRILVLGEDPRESLNFRDHLSYMPQKFGLYEDLTVLENLQLYARL
                     RGIPQAESQENIKRLLSFTGLEPFTGRLAGHLSGGMKQKLGLACSMLVRPKLMLLDEP
                     GVGVDPISRRELWKIVCEQRDQGVGIVWSTPYLDEAERCDYVLVLYEGQVLFFGQPSE
                     FTARVKGRTFLADVPPHERRHVVRQWLEDPRVVDTRIQGQRVRVVVRENGEDAGKKLP
                     GLRRVTTDPRFEDAFIDALGGIPKERVTFRRNGNHEAVVNGREEVVVADGLVKKFGHF
                     TAVDHVSFRVRRGEVFGLLGPNGAGKSTTFRMLCGLLAPTEGRATVAGVDLRKARAEA
                     RRHVGYMAQKFSLYGNLTVKQNLQFFGGVYGLRGDNLRNAIDWALEELELKPFADQNT
                     IQLPLGFKQRLALACAIIHRPDVVFLDEPTSGVDPLARREFWLRIDQLVEQGAAVIVT
                     THFMDEAEYCDHLALINRGRIIAMDTPDAVRAMVQSPEHPNPTLEEAFIELLIRFGEQ
                     EGVETNPNTLLNDEKRSEEKTK"
     gene            complement(14970..16037)
                     /locus_tag="PLD05_07735"
     CDS             complement(14970..16037)
                     /locus_tag="PLD05_07735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOP77367.1"
                     /translation="MKKRLILIVIIVAIVALSLWSLQRYARPTVDQGLHVQAWWTAIM
                     NGGTPEYRELHGNVEIREVRIGFKVPGRLARLYVDEGDTVGRGSLLAELETAEFLDAV
                     EQAEAALAARRAELLALENGSRPEEIEKAKSLAEAARVAVRNAEVSLRRAKDLAPKGA
                     VSQETLDNAQAAYDQAVANYQAALATQRLVEVGPRQEDIDRAKALVRQAEAVLKEAQR
                     RLADTRLISPIHGVVQVRIYEVGDYVGTGAPVFSIARQDEVWVRTYVAERELDHIRPG
                     TVVEVLTDGGNRFSGQVGFISSVAEFTPKSVETREVRTTLMYRVRVLVKDPSGKLRHG
                     MPVTVRIRRDETTEASTGRQR"
     gene            complement(16089..16790)
                     /locus_tag="PLD05_07740"
     CDS             complement(16089..16790)
                     /locus_tag="PLD05_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOP77368.1"
                     /translation="MNDSPQGSKGRRSPKKRGDARQRLLEAGIEAFARFGPDEVGIRR
                     LAAAAGVNSAALSYYFGGKQQYYRAVLRYLMRWVGQSIRQAAMTARAGLAASPDACHA
                     RQLLRIFMREVVQTVLANPQAEAVAAIIWRELLRPSSGFDIVYEQMIRPVHEIITELV
                     AAAMGVTSDDREAVLLAHSLWGQVAIFRLGFHVLRRRLKIRGRQLSNEWVEAITSTVD
                     LVVDRLLSSPPETRR"
     gene            complement(17049..18902)
                     /locus_tag="PLD05_07745"
     CDS             complement(17049..18902)
                     /locus_tag="PLD05_07745"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOP77369.1"
                     /translation="MAIPAFFSNRAIYETSGASLKGRRFGLKEYLLIGRTMACWIATA
                     AYLFVSGLSFAGPQDADWRNLRLGNVIGEAGYADQPYVVVLPNGEWLCTATIGPGKEG
                     QTGQHIAAFVSSDKGKLWSSPIPIESPQGPEASWAVPLLTPFGRVYVFYTFNGDNVRT
                     LNEKPIRADVLGWYVYRYSDDFGRTWSERYRLPMRVTACDRTNDWKGSVQLFWGICKP
                     QVAERRVLFSFTKLGKYMLELGEGWVFCSGNILEEREPTRLQWELFPPGDRGIRNDQF
                     GSVQEEHNLVWLGENTWYCVYRTTLGYPAQTYSFDGGKTWEVPSLMTYEPGGRPMKNP
                     RACPKLYRTSEGRLLFWFHNHSGTDFFGRNPAWLSAGTVKNGRVYWSQPEILLYDPDP
                     QVRFSYPDFFEDEGRMFVTETQKTVARVHEIDREFLNVLFQQGTLASLPKVAPLVEIR
                     SESPEGPKPLTRTIDLAEDSGFSVEMWIRLDHSQPGRILVDNRDATNRGFVISLGEEN
                     SYQVELNDGLHSFAWSSDPGTIQVGKRQHVVIIVDSGPKLMMFVVDGQLCDGGEHRQY
                     GWARWTDDLRQVTGQSIWINPALKDCIHSLRIFGRPLRVSEAVSLYRAGSH"
     gene            complement(19052..20518)
                     /locus_tag="PLD05_07750"
     CDS             complement(19052..20518)
                     /locus_tag="PLD05_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77370.1"
                     /translation="MSRLGLMTQVLLSLALFNWVANSLFFHSLAEEVIPSSWEEFQRH
                     GPPLLSLGHIRTRSASEIKNSSWSVGCETLDRDYAVYDNYRDYVGPLGAVRARIQAGW
                     AKCEKAKGIYEWAWLDHVIEDLLHQGVHPWVCICYGNPIYPGGGTPNLGGGLPQSDEA
                     LAAWDRWVEAMVKRYRGRVIEWEIWNEPDLGRTEERLPQYVKLFVRTAETIRTVDPQA
                     KILALAVCSPTSSMIAPFLKELQRQDKLHLVDAITFHGYTRRPEDLYRGVVSLQNLVR
                     EFDPRIELIQGEAGCPSVEHPIHALAKYPWTEFSQPKWYLRRMLGDFSHRLRSSVFTI
                     VDIRYPEVLLSMGLLRANDNKEVLYKKPAYYAVQHAMSVFDSSVRPSGPVDVSYSGER
                     SVRVVACQKGDQPLLAIWFDGEVPSDSLDWVPVDLSLENVRFRSPVYVELITGRAYHL
                     PPDSYRQEGPRLTFKNLPLWDSPILIMEQTQLELRSDQ"
     gene            20939..>21612
                     /locus_tag="PLD05_07755"
     CDS             20939..>21612
                     /locus_tag="PLD05_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77371.1"
                     /translation="MLMMRSESVCRGKQCHLIWSLVLVIGTVGLPLLGAESPPFPGER
                     GERLDLACREDIWTREPTTFLEKFVRVPRDQVVGFALYTHDHGVLKITAQLYPLKPEE
                     PREVRLEFWQNGTWREVARAPVVYPGWSAHFRITPWDDTQRVRYRVCHGTAASFEGVI
                     RPNPVDKDEIVVASLSCNSNADRGDRQEIVANLLKQDPDLLFFAGDQSYDHQEHTAAW
                     LLWGWQ"
BASE COUNT         4974 a         6383 c         5468 g         4687 t
ORIGIN      
        1 tcaggcacaa acggatacat ggcggcgtct gggtccgcac tgaagctgag gctcaggggg
       61 cgtccctgta cagccaaccg ctccacggac ttgccccacc gccacaagtg ataactgaca
      121 ccaccggcac cgagaatgac aaaagagagc ggaataagga ggaccaacca tgcccaccac
      181 caaaaacccc ggtcaatcac ggcgatttca gggtcccgtg gatcatacca gcaggtaata
      241 atatcgtcgg ccttgaagcg ttgaagcgtc ttgagggccc gttccttgtc caccgaatag
      301 ccatttcctt gaacagtgtg gatgtcatag gtccaaagcc ggtaagtgtg gcctccaacc
      361 ttgtattcaa tgcagacttc aggccgatac aaggtttggc catcgcggac ggtcgtcccc
      421 acgcgggttt ccaagattcg tgccggagaa ggacgaaaat cgcgatttac ccaccattcc
      481 ggcaagacga acaacgaaat cgtcaaccag aatccgacac agcccaacag gaacaagacg
      541 ccgaaaaacg ccccctccca gattccggcg atctgctcgg atccggttcg acgattaccg
      601 cgcttctttt caaataaacg aaaccagcga gccaaattgg tgtctttcca gcctcagcgt
      661 cgttgccgac ccgccggacc gatttcgcca aactacttca catttcttcc cgtccgtagt
      721 tcttttgagg agaaacctat ctccccagcg cgttgtcctt gatttcagcc acagtgggag
      781 cctgcttgcg ggatggccgt caagaaagct ccgacgtgtt ccccactgat tcatcatgac
      841 cctacggagt cggcaacctt ctccgtctcg ttattttcct caaaaacggg ggtcgctgtc
      901 gagtcgtcga acacgatccg gaactggatg acgctcgact cagacatctc ggggacaaaa
      961 ggtgtaggac gatcttccag tcctaaacta cggagacggc tgcgacgaaa gaatcccagt
     1021 cgctccaggg cgagaaagac ttcttccagc gtcttctcgc caaagttcgc aatactgaga
     1081 agtcgctcgg gcgaggtatt gagaagctga gcaaccgtga ggattccgtt ctcctcgaga
     1141 caattcgtcg tccggactga caggccgatc tctgccgtgc tcatctgcag ccgcttgttc
     1201 agctcggcct cgtacagtgc tgctgggctt aagcgaacgc gtgaacccat aacgttccct
     1261 ccttggacga ccaaaaagcc atggctatcc gttgccggtc aaggaataca agacactcac
     1321 tcgctgcctt atccttctgc gagggccgac caccgacgac tcgctacgcc tcggtttgca
     1381 acggaattgc ctgaccccta acattcctcc cagcctcgca gaaggggtct cgctcggtca
     1441 acgagcaata ccgaatatag caaatgtgcc gattttaggg aatacgcgga gcgccaaagt
     1501 ttgcgaaagt tatccagaga cccgacttgc cggccatcgg cggggacctt ccccccaata
     1561 agggcatcct cgcgtccgtg agggggcttt gtcccaaaag gagccggcgc gttattcgaa
     1621 acggttttgg gacattgact tccccgattc agaccgtgtc aacccgccgg acactcactg
     1681 atcctggcgc cgggtgcctt cgcgacgggc cctccgttgt tctctgattt tccgctgctc
     1741 atccgaaggc aaaacgtgaa agcgaatgcg gaagacccga tgggcgcata attggtcgca
     1801 gctataaagc ctctcgatct ccgcgcgtag ctcctggact gataaaaagc catcgggaat
     1861 ggcgtcttca tccgtcaggt cgttaagggc cacctcatct acggaatcga cgcggatgta
     1921 cccaaccccg gggatgtaac tgagctgccc cgccctcatg tgccgaacct tccacacgcg
     1981 gattgtctgg gtcttacgac ccgtgcggat atcatccagg aatttctttt tgaacagcaa
     2041 catgtcacat caacggggag caaaaacgat gagcatgatt aaaccaccaa gtacgataat
     2101 ggccgccccg atggcgagtt gcagccaaaa acgtgcagca cgggcggccg ctgccgcggc
     2161 catctcttct cgcttgcgcc gatattcctc actggcctcg tattgtggtt cgaatgtcgt
     2221 ctgacatatg gggcacataa ccggcctgcc gagcatgtcc gcgggtgttt ccagaatatg
     2281 gccattagga cacggtatgt ggaaaacact ctgactttcc tccgccccca gagacacacc
     2341 tatcgcacgc tctggtccgg ccgacagctc aaatgctccc ggttcgacgg agaggcctga
     2401 tacagaagcg aaactgggag aaccgcggac ctcgccgcca atggcatctt cttgtccatg
     2461 cccaagcaga gcgctctcgg taggaacatt tacagacgcc tgacgggaat cctcttgaga
     2521 tgacgcggcg gttggctgat tcaaaggcgc ggggataagc atttcgactc cgcaaaatgg
     2581 gcagaccgca gcctggccta cctgctcgat ctctgcctcc aaaatatgac cctgggggca
     2641 aaagaaatga aaccccatga cgtccccgtc cgtcacgcat caccactggc ggaacatggc
     2701 accgcgtgca agtgcctccg ctaccaccac tgtgttacca agttttgcga tccctgacca
     2761 atacttcggt cgtcacccac gtgtcattct aacaaccgca cgcaaccacg catatatctg
     2821 tggtccgatc acgctgtccg ctgggttgtt atgcgtgtgc cgcccaagaa cggtttccct
     2881 tgtcatggca ttgtcacatg atgatggact gcgaccggct ggccgagtat gacagatcgc
     2941 agaatcctac ccacatcggt gttgtccacg tatttaccgg agattcccca gacttgagcc
     3001 gcctgggcat cttcaccgcg cacaaaagcg gcaaaccttt ctgccccaac tccgcgggcg
     3061 tacaacggta ccagcgagtt cgtgtgtttc gttgcgttat aacgagccgc gggcatcttc
     3121 ccagcaccag catcttggat gggggcaaac ggtttcttat cagaatccgg cccccagaga
     3181 cagccgcatt cgtgatctgc cgtcaagaca accaacgtct cgtcccagga gctgttctta
     3241 ttcacccaat taacgacaac ctcgaccgca gccaaaaaat cactttgttc ctcgatcatg
     3301 cgatggagtt gattggcgtg attagcccaa tccacagcgc cgccctcgac catcaggaag
     3361 aaaccatcgg gatcattatc caacacattg agtgcccctt cgaccatcac tgccaggctt
     3421 ggcacgttgt catttaatgg ctcggcgaac ggttccccag gcaagaccac tttgatcaag
     3481 cccccgcgtg cttgctggag agtcgtggcc acctgggctg ttcccagcac cttcttcggt
     3541 gctgagcctg tggtaagttt ttcgaaatct tcttttcttt caatgagcgt ccacccacct
     3601 gggtgctgac ccgatgtcag tttttcccat tcttgccgcc ctccaacata acgataatcc
     3661 gcttcttcca acttaccctt ttttggttga ccgtttttgt caaacaaagg atggcccgcc
     3721 cccatgatca cttgcaaata gggagccgcc agcatttcct gagcgatctc agcataattg
     3781 tcccgatctc ggttatgggc accacctaaa gctgccggtg tcgcatgcga ccactgcaca
     3841 ctcgtcacca cgcccgctga tttcccagcc cgatacgcta cttcaggaag cgactcaccc
     3901 atcatggcct ggtccatatc ggaccaattg atcgcgttat tatagctctt ttggcctgtg
     3961 gaaagtgccg tcgcggccgc cgctgaatcc gtagcggagc tcgtcaacca cttgtagccc
     4021 gactcagcat cccaggcctt cgaaggcgag tacacgacat tggggtcttg ttgacccgtt
     4081 ttcttcggtt gagtcgccgt gttcaaagga aatgtcgtac acgccagccg aacccacccc
     4141 ggttggtcgt agatttcgcg ccctacacgc ccgcggtaca tactcgttgc caaccacgtg
     4201 ttgaagcccg ccccgtcggc caccattaag atcacatttt tggcgcgaac ggacgattct
     4261 tcggcgaaaa taaccgtacc agtttgctgc gcagcgccga caaaaccaaa aacgactacc
     4321 agccaataga ttgcaatacg ggtcctcatc aagactctcc tttcgctaag accgcttggg
     4381 caagaaaatt cttctgtctt gtaggtcaag acacttaatt ctggctgaaa ttcctcgcag
     4441 atcaacgggg caaataccgt cttaacgaaa tattaaccaa ctgactgact agtgaggctg
     4501 atcggccgag gcgttaagtt cttccatcgc ggcatgaaag gcctgggcgg catccaaggc
     4561 acgctgcgag accaaggcag ggtgtcgttc gccgacaagt gcccaacaga agccgccagg
     4621 ctcaagaacc catgtggtcg cgaggtaacc aacgacgccg cccatattgt tttggcggga
     4681 atagcgtatt agtgatcgaa cggcatcaac gttattccat ccggccggcc aaactcgaaa
     4741 gcccttctcc tggaaatacc ggaccgaagg atattcctgg tgttttccat agtgccagtc
     4801 acaaatgacg atatccttgg ggatcatgtc gatagcgggc gccgtgccat tacggctggt
     4861 ctcccaccct ttgccatagg gaaacatttc agcgtcgagc aggcggtcgc cccacattag
     4921 catggtcagg tttcgttctt tgaccaggtg ctggtggagg tcgttgactg ccttcgcgaa
     4981 gacctcggcg ggagatttgt cgcggcaccg ctcgcaattt ttgctggcga tcaccaagac
     5041 ttcgtccatt cccacatgaa aggcatcggc cgagaaggcc tcgatcagct catcgatcag
     5101 actaaatacc acgccattaa ctcccggatg caatgggcac caactccgca actcggtcca
     5161 ccgctggcca ggcggcgcct cgggcggttc ttcgaattcc cgatactggg tcagaagtgg
     5221 atacaaagtg tttcgccaac tttggtgtcc cagacaattg aattggggga tcacacgaat
     5281 cccgtactgg cgacagtgtt ccaccaaatc ctgcgcgtcc tttttcgtaa tgaacttcgt
     5341 ttgacgcaat tctaggtggc tgcgatagtc gaagttatat cccacctcaa aaacgatcac
     5401 gttgactccc ataggatgga acaccttagt gatcgcttcc ttcaacggga taagcccctc
     5461 cggccctccg gccaaccccc aagccatgat gtgaacacct cgccactcgg taggatttgg
     5521 ctccgctccc aggccctgaa gtgggctgac cggtgcaact gtcaggacag agaccatcaa
     5581 catgcccaaa ataagcacaa aacgccggtc aatttgaccg gcacaagcga ccaaccaccg
     5641 cgccacggac ttaatgatgg acctcatcat cacaccacct cctcgacatt cgaaacnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcc tcaacctatt gatctgaccg
     5821 ttagtcctcg cgctcaccaa ccagtgtccc ggtagtacgc tgagtacaag aagtgccatc
     5881 gcaagccaat aaaaacccgc cggaggatag ttcgctcgag acaagttcag gtaccaggcg
     5941 cttccgaccc acgcccagcc aaggcggacc gcaatatcca caagcacggc cgtcggaagc
     6001 aagttcgcgg caacgttgac ccaccatttg gcaactcggg aggcgagctc ctcggcctgt
     6061 tcgtccactt cgttgcggag ttccaaaagg agttcttgat cggtctcctc gttaagtagc
     6121 ccgacaacga tctgctccag actggttttc aaggttgtgg ccgggctgga tgacggaggc
     6181 tccgcagtat cgggcacttg agaagtgagt acctcggcca attgatgatc ttcgagcgtg
     6241 cggtggatat cggccgccag ttgtttcaga cgccatttgg tatcactgcc gagcttctcc
     6301 agaacctcct tcaacggcca gatcccggtt acaaacgccc gcaaagcggc catagttgcc
     6361 ctcgctatcc cccacagcga aggcccgtaa acgcccaggc gaagcaaccg gtaaccggcc
     6421 atcaggtagt gccaacgggc gtgcacccaa gccgtcaacg acgcgattcc ccacaatcgc
     6481 ctggcaaggc tttgccacac ttgctgccgc acaatcgcac gaaaggccgc cgccacccgc
     6541 ggttcttgaa agcccttctg atatacacgg cgaatctcct ctgcaaatcg ttcttcagcc
     6601 tgccttagct gcgcgatctt gtcctgcaaa ggagccaaca ccgtcggcga agtcgcatat
     6661 tgcacatgag ctaaaaacac ttcatctcgc aactggcgga ggtcttggcg ataatcatgt
     6721 tcgattaact cgcggcgcaa aaggtcaaac tggaagcgct ggggatgctt ggccgaaacg
     6781 acaaacctgt ttcgctcgtc cggatcaaag cccagctcct tcaaccgtcg gtcccagtcg
     6841 cgacaaactt cctccagggc cccttcaggg atttcatcag cccggttgag gacaaagagc
     6901 caacgttttc gctcggccca gcgacggacc tcctcatgga cacgaaagtc cgcccgtttc
     6961 tccggagatg taacatagac gacgatatgg ctttgttcca caatctgacg cgcaatgtcc
     7021 caatgttctg caaggacacc atcgatatcc ggcaaatcgc aaagaaccaa tcgccccagc
     7081 aatggcgatt cgacgaactg tgggcgaagc tcttccggaa cggcaaccaa cgggcgacac
     7141 gcttgtgaga ccgccacgta gggcgtcttc gtaaagcaac gctcggcgtc ggaaacgggg
     7201 ctggcacctg gccgacggat tagactatta aaaagagtcg atttgcccgc gccggttgga
     7261 ccaagcagca cagcctgaac tacggcaggg actgtttcgg cttggaggta ctgaagggca
     7321 cggtcaagtc gcaccgtcat ctcggcggca gccggcaagt gacagaggcg gccaactgct
     7381 gcctttaacc gggctactct ttctacaagc aattcagccg acgaaatatc caccgtgcct
     7441 tgggccatca ctccgtctcc tttgcaggcc ccaatgagga aaacaccgtg gcatcctctt
     7501 ctctcgcatc acggacattc accaggacct gctgtagtgt ttcgcatgct cgaagtgtct
     7561 tacctaaatc gtgcttggcc agcctactaa gccgctcctt ccaaggtttg aagagttccg
     7621 cctccaggat ttcgccgaga aactgggcca actctttcgc acgatactcc gcccaaactt
     7681 ggtacgcaga ctcggcaact tgttgcaatt tccatcggcc gaaatattgc agaagccatg
     7741 ccgctacgcc gttgacggta gccaggcccg aaagcgtacc aagtgtacca atgagtcctc
     7801 cacttatgcc caagtctgcc actaaaaacc ctaaagctga aatccccagc gtcgtcggga
     7861 tcacactcac cgtcgtagct gtccacttgt tctctcgcac ccaatgcgca agttcttcgc
     7921 ggaccgatgc ttcccaaccg atcccggcaa ggggtaaggg ttgtgactca atcacgtcgc
     7981 gcgctttctc gcagcgttct gcggaaaaga tcccgttcgc ggtggcttct tcaggaaaat
     8041 ggctccgaat ctcgtgaacg agatgttcca ctgcctggcg caatcgcttc cgctctaact
     8101 gcgactgatc acaaatatca acagtctcct cctctcggtt ggacccgcct cctaggctaa
     8161 agaaatctct tactgtcatt actttccccc ggatgcggtt tgtgaccccg ttcatccatc
     8221 cgagggactt ccgaatcaag gctgggacac gtttttcgca ttcttccacg gccaattgca
     8281 ccagccggcc gacgggaaac atcccctcga caacggtcag ggccgcacgg tgaatgagtt
     8341 cctctattgt ccgcagattt ccttccaatt catttcgttc cttctcggcc ttttcggcta
     8401 gccgtttcga gtaaaagata ccttcgcgga tggcttcgat cagtccccgc acgacaaggt
     8461 ggtgactgtc ccgcccgacc aagagtgaca aaaagccggg agcagtttca tccaagggga
     8521 taactctctc gccggacaac tcctcgctcg aaagaactcg cggactggcg tagaagaggc
     8581 tctcggcgag aaactcggat agtgtttttc catcaacgcg tttttgatca aagcccaggt
     8641 cgtgccgctc tcgtaccagt tgcaagagat gtctcctctt ttcccgagcg gcctccggct
     8701 ccgtctttgt gaagacatag accaactggc ctgctaaccg gcagcaccgg gccagcattt
     8761 ccaccgtccg ccgatccgag taggactcac tataagtgag gaacaagaca agctcggctc
     8821 gggcaagcat cctctccgcc cgttcccagt tctgcgactc cactgtattg aaatccggca
     8881 catcagccag aataaacggc aatccttgag gatcctcctg cggacagaaa aacacgcggt
     8941 cgctgcttac ccgcaattga ttggggtctt ggagctctgc gagctcacag gagggaaaaa
     9001 caccttccaa gaattctctt tcccagagct ttttcggtac ggccacgacg caagcgcagg
     9061 tggtcggccg ctgttcttcg cccgctggaa cattcacccc tgtcagaaga cgaaaccagg
     9121 tgctttttcc acctccactt ggtccgaaaa gcacggccac aagcggcgga gataggcctt
     9181 tgttgcggca gaactccaag gactggaaac gttttcgcat ggcctcccag cctggtggct
     9241 cagcgagttc cccaagagca gctttcaatt ggggaatttc ggaaagcaac caagccaggc
     9301 accggtggac ctcagcgatg ccgtatgttg ggtctcgcac cggcaagtct tcccccacga
     9361 tcatgagctg aaatccgtct tcatgctaca tagatgaaat cggccagaat gttgacctcc
     9421 ggaactgccc cagacacggt ttctctccca ttaacaagcc aaatgggcga ctatcgtgat
     9481 tataggtcgc atcgcatccc ccaacaaatg gcggggctac aacggttctt cgcgagaaag
     9541 atagccaaat cgcaagttcc gtccggggcg attcaaacct cgctgaagac ggtcctggcc
     9601 atgggaggcc aaccccgcaa gacctcaagg agccggtggt gccaagtttt aggctcatga
     9661 gagcgcccca cccgcgtggg gcgccactat cgtgcccatt ccaaaaggca aatgttgaca
     9721 gtccttctgg ccctggttag agtagtgttt ggttctagcg gcagttcgtc ccaagtcaca
     9781 ttttgacttt agcgcaaaag gaatggggta tggcttcgcc cggcgggctt gaggtgttcg
     9841 gggcgttaat caccttcgtc aacttcgtcg cagcgtgttt tttactagga ctgatattct
     9901 tcagaagtat tgcagggggc tctctcgttt tacgctcctt cgaattgcat tcgcatccta
     9961 cctctattca gtcggatgtc attcatgtgg tgggacggcg agctggtctg atttcttatt
    10021 ttctaaccct gctagggctt tcctcgacgt gtacatttcg ggtaacccac caatatgcgg
    10081 agttcgtgga ttccagcttg tttggcaaga cacatgcaat ggttccgctc caacatgtag
    10141 tgtgcgtacg gggcggcgtc gcacggccgc tgcttgcgct ttttttcgtg ggacttttcc
    10201 tcatcaacgg cttccttttt ttgatcgcga ttgcctttga attggctttt gctattctct
    10261 cccttgcctt ctttacggca gccggttcgt gtctagtcta ttactttcta agaaagaaat
    10321 tcttcgtgtt gattcgctct gaggcgggga cagagatcgc tatttcgttc atgcccagtg
    10381 tcctcgaagg ccaaacggtt gactttccga aggccttaat ggcagtggag gttttaaaga
    10441 accttgccct ggacgcgctt catcgaaccc cgcccacgac cgttgcaccg gcttttgagg
    10501 aaacccctgc gtacccggtc gaggatcagt cgcaggtttc catatcgcca agggttaccc
    10561 cccctccgct taccccggaa gccgtgagcc ccatctttac cggcccagaa ccaccaccga
    10621 gccatgaacc cgagccggag gaggtcttta cgccccaggc ccccgaagag gtcgctatga
    10681 aagccctacg agaggccgtt aagacctaca acaatggcca acacgaagaa ggcatttact
    10741 tgcttcagga agtcgttcgt ctcaatccgg gaaccaaggc tgcccgcatt gccgagaaac
    10801 atttggcaaa actggggcgc catgcgtgag cggagcacca agatcgttga cttcttacca
    10861 gcatctgtca tcccggtgct gttgcacgtt actaggcaga gctggtcgtg gtgttctcta
    10921 ttctaatcgg tgacggaaca accatgcggc gctgaaaagc gtaaaggctg caatgggtaa
    10981 gagaggggca acataaggaa cgattacatc gaggcttgga tcttcgagaa acaccatccg
    11041 gctaatggcc accatgtagc gaagcgggtc aatgtccgaa agccaccgga tgatcggatg
    11101 gatgttttct accggcgtgg caaaaccgga aagaaggacg gccgggacca tgtacgtgaa
    11161 gcttcctaat agggcttgtt gttgcgttgc agccagcgaa gaaatccaca acccgatgcc
    11221 cacaatcgcg aggagaaaca caaacatggc catggccagg agcacgaggg agccacgaaa
    11281 agggatgccg aaaacgatga tgacagcacc taccatcaaa cctccctcca aataggcaat
    11341 gacaaacgcc ggaacggcct taccgataag aatctccgcc ggtcgcaaag gtgagaccag
    11401 cagttggtcg aacgtcccca gttcacgttc ccgcgccacc gagagactcg tcaccagtag
    11461 tgccaccaga ttggtcaaaa tagcgaggag tgccggaacg gacgaccaca ttggggacgt
    11521 attttcgttg aaccagaccc gagtgaccgg gacaatttgg tccacacttg agtgtcttgg
    11581 tggcatgttc aattgttgtt cctgtgcgaa ctgacgaaca attcgaccga tgtacgcctg
    11641 aacgaccatc gcagcgttgg aaaggcgtcc gtcgagcaag acttggacga cggggatttg
    11701 tccagattcc agtgatcgcg aaaagtcctg gggaatatgg acaatggcca gggtttgttg
    11761 gctatccaaa acttctcgca cctcttgatc gttactgcag gaaatgacgc gagagaaggt
    11821 cacgggaata gcttcgaatc gtgcaactag ttcccgccct agaacgccga catcttcgtt
    11881 gtagattccc aatcccgcgt tgcgcacttc caacgtggcg gcaaagctaa agaccaatag
    11941 ctgcacaagt ggcgggacaa tcaccaccat ccgactggct gggtctcgaa agatcgccag
    12001 aaactcttta atgaccaaac gccggatgcg ctccagcatg aaccttccct tactccaaag
    12061 tcaaacgcgt cttacgaatg gttaacatcc aaaggattgt ggcaaacacc gccaagatgg
    12121 ccgtatcctg cagcacgatc tcccacacat ttcctgctaa aaaaagtgtc cgcaacgccg
    12181 acacaaagta gcgtggcggc aatatgtgag tgagtaatcg aatgggggtc ggcatactgt
    12241 taatttcgaa tacgagacca gaaaggatga aagagggcaa ataccccaca acgagtgccg
    12301 cctgggtagc gacaaattga ttacgcgttg ctgttgaaat gaaaagccct agccccagcg
    12361 caaccgtcaa ataaatggct gaaacaacta ttaaaagccc ccaggatccc cgaaacggaa
    12421 tgtgaaagat aatgaccgaa accaaggcca caagcatcat cgccgtcagc ccgagggcaa
    12481 aatatggaat gaacttgccg agcagaaact cgcctcgagt gattgaactg gcgagaaggg
    12541 cctccattgt gccacgttcc cattccctcg ccacgaccaa tgacgtaaga atggttccaa
    12601 taagagtcat gatgacggcg atggaacctg gcaataaagc agcccgcgag tccaaattcg
    12661 ggttgtacca aacccgactc tctaaatgaa tggtgtcccc gattagtcgg ttacctgttt
    12721 cctcagcacg atgccgatgc tcggtttgca ggacgttttc caaataactc ctcacaattt
    12781 ccgcagtgtt ggcttcacta ccgttcacaa gaatctggac tgtcgccgat ccacgactca
    12841 agttcgctgc gaaatcctgc ggaatgatga ccaatccctt aatccgtccg cccaccaaga
    12901 gcggttccat ctcttgccga tgattggcct ccctcacgat gaaataacgg cttccgcgca
    12961 gggaagccac cagccgctcc gcatcacgcg agggtgattc gaccacgagt ccaatcggta
    13021 ttgatcccag gtcaagggaa atcccccatc caaagagaaa caagaggacc accggcaaga
    13081 ggaaggcgat caccaggctg ctcggatccc gaacgatctg ataactctct ttccgcgcta
    13141 acgccaaaac acagcgaagg cgaaagcgtc tcgcactcag tcggacgttc tgtcgattga
    13201 ccgcgttcat ttggtcttct cttcagatcg tttttcatca ttcaaaagcg tgttggggtt
    13261 agtctcaacc ccttcttgct ctccaaaccg aataagaagc tcgataaaag cttcttccag
    13321 tgtcggattc gggtgctccg gagattgaac catggccctc acggcatcgg gagtatccat
    13381 ggcgataatc cgcccgcggt taatgagggc caggtgatcg caatactctg cttcgtccat
    13441 gaagtgtgtg gtcacaatga cggcagctcc ctgttccaca agttgatcga tgcggagcca
    13501 gaactctcgc cgggctaagg gatccacgcc gctggttggc tcatccaaaa agaccacatc
    13561 cgggcgatgg atgatggcgc acgccagagc caaccgttgc ttaaagccca gtgggagctg
    13621 gatcgtgttt tggtcggcaa acggcttaag ctccaattct tccaaggccc aatcgatggc
    13681 attgcgcaaa ttgtctcctc gcagtccgta caccccgcca aagaattgga gattctgttt
    13741 gactgtgaga ttaccgtaaa ggctaaactt ttgggccata taaccgacgt gtctgcgtgc
    13801 ctccgcccgt gccttgcgaa ggtccacccc cgcgactgtt gctcgccctt ccgttggcgc
    13861 aagtaaaccg caaagcatgc ggaaggtggt actctttcca gccccatttg gtcctaatag
    13921 accaaagact tctcctcgcc gaacgcggaa ggaaacgtgg tccacagcgg tgaagtgacc
    13981 gaattttttt accaggccat cggctacgac tacttcctcc cgcccgttca cgacggcctc
    14041 gtggttccca ttccttcgaa atgttaccct ttccttgggg atgcctccta aggcatcgat
    14101 gaaggcgtcc tcaaatcgcg gatcagtggt cacccgacgt agcccgggaa gctttttgcc
    14161 agcgtcctca ccgttttcgc gcacgacaac tctcacacgc tgtccctgaa tgcgtgtatc
    14221 cacaacgcga ggatcttcga gccattgtcg gacaacatgg cggcgctcat gcggaggaac
    14281 gtccgccagg aaggtacggc ctttcactcg ggcagtgaat tctgacggtt ggccaaaaaa
    14341 gagtacctgc ccttcgtaaa ggacaaggac ataatcgcag cgctcagctt catcaaggta
    14401 cggtgtgctc cacacgatgc ctacaccttg atcgcgctgt tcacagacaa ttttccacaa
    14461 ttcccgccgt gaaatcggat ccacccctac gcccggctcg tcgagaagca tcaacttggg
    14521 gcggacgagc atcgagcatg ccaagccgag tttctgcttc ataccaccgg acagatgacc
    14581 cgcaagtcgc cccgtaaatg gctctagccc tgtaaatgag agaagccgct taatattctc
    14641 ctgactttcc gcctgaggaa tgccgcgtag ccgagcatag agctgaagat tctccaagac
    14701 ggtgagatcc tcgtacaacc cgaacttctg cggcatatag gaaaggtgat ctcggaaatt
    14761 gagagactct cgaggatcct cgccgagaac caaaatccga ccttctgtgg ccataaggag
    14821 tccggccgcg agacgaagaa acgtcgtttt gccggccccg tccggcccca ccaagccggt
    14881 aatagcacca gcaaggattt ccaccgtcac atcccggagc gccggccttt gcttgggcga
    14941 gaaacgcttg gtcacgtggt caaaccgaat caccgttgcc tcccggtgct tgcctcggtc
    15001 gtctcgtctc ggcggatgcg gactgtcacg ggcatgccat ggcggagttt acccgagggg
    15061 tcttttacca gcacgcgcac ccgatacatc aacgtggtgc gaacttcgcg agtctcgaca
    15121 ctcttagggg tgaattcggc caccgaggag ataaatccta cctgccccga aaaccggttg
    15181 cctccatcgg tgaggacctc tacaaccgtt cccgggcgaa tgtgatccaa ttctcgttcc
    15241 gccacgtacg tccgtaccca gacttcatct tgccgggcaa tcgagaagac gggcgcacct
    15301 gtaccgacgt aatcgcctac ttcgtagatc cgcacctgga cgacgccgtg aatcggcgaa
    15361 atcagccgcg tatcggccaa tcgccgttgg gcctctttga gtaccgcttc cgcctgacgg
    15421 acgagggcct tggcgcgatc gatgtcttct tgccggggtc ctacttccac gagccgctga
    15481 gtggccaaag cggcttgata attggctaca gcttgatcat acgccgcctg ggcattatcg
    15541 agcgtttctt gagaaacggc ccccttgggc gcgaggtctt tggcccggcg aagtgacact
    15601 tccgcatttc ggactgccac ccgtgcagcc tctgcaagcg acttagcctt ttcaatttct
    15661 tctggtcggg aaccgttctc caaagccagg agttcagccc gacgtgcggc cagcgcggcc
    15721 tctgcctgct caacggcatc gaggaactca gccgtttcga gctcggcaag cagcgaacct
    15781 cgtccaaccg tatccccctc atccacgtaa agccgggcaa ggcgaccagg aaccttgaat
    15841 ccaatccgca cttcacgaat ctctacgttt ccgtgaagct ccctgtattc aggcgttccc
    15901 ccgttcatga tggccgtcca ccatgcctgg acatgcaatc cctgatcaac cgttggccga
    15961 gcataccgct ggaggctcca cagtgacaga gccacaattg caacaataat cacaatcaga
    16021 atcaggcgtt ttttcataac gggactgctt tgccttcaac tgcgttcttg cgttcgtatt
    16081 acacaccttc atcgccgcgt ctcaggaggg gatgagagaa ggcgatccac cacaagatcc
    16141 acagtgcttg tgatcgcctc cacccactcg ttggacaact gtcggccgcg aatcttcaac
    16201 cgacgtcgca aaacgtgaaa acccagccga aaaatggcga cctgtcccca taagctatga
    16261 gccaacagga cagcctctcg gtcgtcggaa gtcaccccca tcgccgctgc caccaactcg
    16321 gtaatgattt catgcactgg ccggatcatt tgttcataca cgatgtcaaa gccactcgac
    16381 gggcgcaata attctctcca aatgatggct gcgacggctt ccgcttgcgg gttggcgagc
    16441 accgtctgca cgacctctcg cataaaaata cgcaagagct gtcgggcgtg acatgcgtct
    16501 ggagatgcag caaggccagc ccgcgcggtc atcgctgctt gccgaatgga ctgaccaacc
    16561 catcgcatga ggtaccgcag aaccgcgcga tagtactgct gtttcccgcc gaagtagtaa
    16621 gagagagcgg cgctgttgac cccggctgct gccgctaggc gacgaattcc cacctcgtcg
    16681 ggcccgaatc gcgcgaacgc ctcgatccct gcctccagca gccgctggcg ggcatcccct
    16741 cttttcttcg gcgatcgtct gcccttggag ccttgcgggc tatcattcat agcaattctc
    16801 cgtcacaaaa cgacctcacc cctccttctg tatcatacca ctgatcaggc gattagtcaa
    16861 ccaattgagt taatcaatcg attaaaacga tcgatcacac acaacggttc taggtccctc
    16921 cctatatgat gcgagccctt agtctaggag accggcacag aagattcgcc gacaaacaga
    16981 gtggcgatca gcggcagact caacttgcta cgagtcaggc agtataagcg ggcgctgatt
    17041 tgaggccatc agtggcttcc agcgcggtag agactcaccg cttcggatac ccgcaatggt
    17101 cggccgaaga tccttagtga atggatgcag tctttcagcg ctgggttaat ccaaatcgat
    17161 tgcccggtga cttgacgcag gtcatccgtc cagcgggccc atccgtattg gcggtgctct
    17221 cctccatcgc ataactgccc atccacaacg aacatcatca acttgggacc ggaatccacg
    17281 atgatgacca catgttgccg ctttccgacc tgtatggtcc ccggatcaga cgaccaggca
    17341 aacgaatgta atccgtcgtt cagctctact tgataagaat tctcctcacc taacgagatc
    17401 acaaagcccc gattcgtggc atcacgatta tccacaagaa ttctgcccgg ctgagaatgg
    17461 tccaatctga tccacatttc cacgctgaaa cctgaatcct cggccaggtc aatggttctc
    17521 gtcaggggtt tggggccctc tgggctttcg gatcggattt ccacaagtgg ggcaacctta
    17581 gggagtgagg caagagtgcc ctgttgaaac agaacgttca aaaactcgcg gtcgatttcg
    17641 tgcacccggg ccactgtctt ttgcgtctcg gtaacgaaca tgcgaccttc atcttcaaag
    17701 aaatcgggat agctgaatct cacctgcggg tccggatcgt aaagcaaaat ctccggctga
    17761 gaccaataga cacgaccgtt tttgacagtg cccgccgaca accacgctgg attccggccg
    17821 aagaagtctg tgccactgtg gttatggaac cagaataaga gtcttccttc gctcgttcga
    17881 taaagtttag gacaggcccg ggggtttttc atgggccgtc cccctggttc ataagtcatg
    17941 agcgacggca cctcccatgt cttgccgcca tcgaagctat aggtctgggc gggatagccc
    18001 agcgtggttc ggtaaacaca gtaccatgtg ttttcgccga gccatacaag attgtgctct
    18061 tcctgaacgg agccaaactg atcattacgg atcccgcggt caccaggtgg aaataattcc
    18121 cattgcaacc gggtcggctc acgttcctcc aagatgttac cagagcagaa gacccaacct
    18181 tcgcccaatt ccaacatata ctttcccaat tttgtgaagg aaaacaggac ccttcgctcg
    18241 gccacttgcg gtttgcagat tccccaaaat aattggacgc tccccttcca gtcgttcgtg
    18301 cggtcgcacg ccgtgacgcg catcggaagg cgatatcgct cggaccacgt gcgcccaaag
    18361 tcatctgaat accgataaac ataccaaccc aacacatctg cacggatggg cttttcgttg
    18421 agcgtccgca cgttatcgcc gttgaatgtg taaaagacat acacacgccc aaagggggtc
    18481 aaaagtggaa cggcccaaga tgcttcggga ccctggggag attcgatggg aataggcgat
    18541 gaccagagct tacctttgtc cgagctgacg aaagccgcaa tatgctgtcc cgtctggcct
    18601 tctttgccag ggccaatagt cgccgtacat aaccattcgc cattgggtaa tacaacgaca
    18661 tacggctgat cggcataacc tgcttcgccg atgacatttc ccaagcggag gttccgccag
    18721 tccgcatctt gcggtcccgc aaagctcagc ccggagacaa ataagtaagc cgccgtcgcg
    18781 atccaacacg ccattgttct tcctatgagc aggtactctt tcagcccgaa acgccgaccc
    18841 ttcagactgg cacccgatgt ctcataaatc gccctattcg agaaaaacgc tggtatcgcc
    18901 atgctacgac tccctttttg gatccttgtc aaagtgatcg cctgcttcta tgtcgtgatc
    18961 gtggagacga tcacgaccgg cagctaaaac gggttctggc cgcggttcga taacgtctta
    19021 tcaagcgggt tggttgccgt cgcaatcaca atcactgatc ggagcgcaac tccagttgcg
    19081 tttgctccat gatcaagatc gggctgtccc atagtggtaa attcttaaat gtcagccgcg
    19141 ggccttcctg tcgataggaa tcgggcggaa gatgatacgc ccgaccggtg atcaactcca
    19201 cgtacacagg ggagcgaaac cttacatttt ccaagctcag atcgacgggt acccagtcga
    19261 gagagtcact cgggacctcg ccatcaaacc agattgccaa gagcggttgg tcgccttttt
    19321 gacaagccac aactcgcacc gatcgctcac cagagtaaga gacatccacc gggccgctcg
    19381 gtcgaacgga agaatcgaaa accgacatcg cgtgctgaac ggcatagtat gcgggcttct
    19441 tgtacagaac ctctttattg tcgttagctc gcaataggcc catcgaaagc aaaacctccg
    19501 gatatcgtat atccacaatc gtgaaaacgc tgctccgcaa tctatgggaa aaatcgccca
    19561 acatgcgccg taaataccat ttcggttgac taaactctgt ccagggatat ttggccaagg
    19621 cgtggatggg gtgttccacg gaagggcaac cggcttcccc ctggatcagc tcgattcgcg
    19681 gatcaaactc ccggacaaga ttctgaagtg acacgacgcc acgatacagg tcttcaggcc
    19741 tccgcgtgta cccgtgaaac gtgatcgcat cgacaaggtg gagcttatcc tgtctttgaa
    19801 gctccttcag aaacggggcg atcatgctac ttgtgggtga gcatacggct agtgcaagta
    19861 tcttggcctg gggatcaacg gtgcggatcg tctctgcggt acgaacgaag agcttcacat
    19921 actgaggtag gcgttcttcg gtgcgtccca aatctggttc gttccaaatc tcccattcaa
    19981 tgacgcgccc gcgatatcgt ttaaccatgg cctcgaccca tcggtcccag gcggccagtg
    20041 cttcgtccga ttgaggtagc ccgccaccca ggttgggcgt accgccacct ggataaatgg
    20101 ggtttccata acagatgcaa acccaggggt gcacaccctg atgaagcaag tcctcgatga
    20161 cgtgatcaag ccaggcccac tcatagatac ctttcgcctt ttcgcacttt gcccagcccg
    20221 cttggatgcg tgccctgacg gcacctaaag gcccaacgta atcgcggtaa ttgtcgtaca
    20281 cggcgtaatc gcggtccagg gtttcgcatc ccacagacca agagctgttt ttgatttcac
    20341 ttgccgaccg agtgcgaata tgcccaagag acagcaaagg cgggccatgc cgttgaaact
    20401 cctcccaact agaagggatg acttcttctg cgagcgagtg aaagaagaga ctgttcgcca
    20461 cccagttgaa taacgcaagg gagaggagca cctgcgtcat taaccctagc cgtgacattt
    20521 tgtcgctccc ttggcatccg gctttgatca atcggtcagc aaagcctcgg acctgctatg
    20581 cgccgtcgct tacgcacttc acaactgtca gcgactgtca tctgcgagcc ttgagcacat
    20641 cctcggttgc agcttgtgct gtgcgatcag tagctgtatt ctagagagca gccattgcct
    20701 aacgcaactt aaggtctatc ccataacctc ccctctcccg ttcggtggga gaggggccag
    20761 aggcgagcgt ggctcgaatc gctgccggtt ttcccgatgg acttcggcgg tcgcttgccc
    20821 tcaccctaac ccctctccca aagggagagg gaacttgcgg ataggctctt aacgcaactt
    20881 gttgtgccag tggtccatat aaacttcgtt gaactgtgac gttggggagg tattggcaat
    20941 gttgatgatg cggagtgaat ctgtgtgcag aggcaaacag tgtcacctga tctggtccct
    21001 tgttttggtc atcggcactg ttggtttgcc actgctaggg gccgagagtc cgccatttcc
    21061 aggagaacgt ggcgaacgac ttgacctggc ctgtcgtgag gatatttgga cgcgggaacc
    21121 cacgacattt ttggagaagt ttgtccgtgt tccccgcgac caagtcgtag ggtttgctct
    21181 ttacacgcat gatcatggtg tcctcaagat cacggcccag ctctatcccc tgaagcccga
    21241 ggaaccgcga gaggttcggc ttgagttctg gcagaatggc acgtggcgtg aagtggctcg
    21301 ggcacccgtg gtttatcctg gatggtccgc gcattttcgc attacaccat gggacgacac
    21361 gcaacgagtg cggtatcgtg tctgtcatgg tacagcggcc agctttgaag gcgtgatccg
    21421 gcccaacccc gttgacaaag acgaaattgt ggtcgcctca ctgtcatgca actcaaatgc
    21481 cgatcgtgga gaccgccagg agatcgtggc taatcttctt aagcaagacc cagatttgct
    21541 tttctttgcg ggtgatcaaa gctatgacca tcaagagcac acggcggcct ggctcctttg
    21601 gggctggcaa tt
//
LOCUS       DAOSUT010000066        21243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1620_length_21243_cov_9.395932, whole genome shotgun sequence.
ACCESSION   DAOSUT010000066 DAOSUT010000000
VERSION     DAOSUT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1620_length_21243_cov_9.395932"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1116..1493)
                     /locus_tag="PLD05_07760"
     CDS             complement(1116..1493)
                     /locus_tag="PLD05_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOP77372.1"
                     /translation="MFAPKTKRTRLVPIVPRLRELLQQAYFAAHEGESLIVTVPKANR
                     HRTLRRIIERSGLTPWRDPFHCLRRSAATDFARSYPINAVAQWLGHGPVVSLKHYQRV
                     PDDLVRKATQDGFGDNRSLALLR"
     gene            complement(1512..2225)
                     /locus_tag="PLD05_07765"
     CDS             complement(1512..2225)
                     /locus_tag="PLD05_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HOP77373.1"
                     /translation="MASLSKHGHTFRLYVCLPDGRRVTLYLGKLPRRQAESFKVHVEQ
                     LAASMATATAIPGPTAVWLAALPDGLYEKLERCQLAPARVRETLGRLIERYEREYVAG
                     LALRSQSRIKNTTKLLSGYFGPKARLSAITQEDAERWRTWLAEGRTEATVRLHVRNAK
                     GLFNWAMTHRLADNNPFQTLVGASMARNDERYVSLEDFERLLDACPNHGWRVLLGLAR
                     LAGLRSPSETDSITWADIN"
     gene            complement(2227..2694)
                     /locus_tag="PLD05_07770"
     CDS             complement(2227..2694)
                     /locus_tag="PLD05_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77374.1"
                     /translation="MNPIALLKRPGVSVDWANGQLKLRFVKGGLTKDEIETLRQNKPL
                     VGGRLLLDKLWQAGYTLHLQRRSDGEGYFIVPRGQAHPGVDFPSLFELYDTFHDQAVA
                     LLLDMCRLGRIHPEDFNSREAWPIQFVDSNDQVLCETKGVATITGNKADKGRG"
     gene            complement(2691..5492)
                     /locus_tag="PLD05_07775"
     CDS             complement(2691..5492)
                     /locus_tag="PLD05_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOP77375.1"
                     /translation="MKPIDTVLTALDRHGCRPKRSGTGWSAQCPAHDDRRPSLSVTEG
                     TDGRVLVCCHAGCATESIVAALGLAMKDLMPDDVLQSHHVTTSPHHQNPVKRAKYVGC
                     ADARPGGNGKPDGQTFPAAEDALDSLRRKLGQESCRWTYCDAAGEPVGLVVRWDKPDG
                     TKDIRPISRFPDGWRIAAMPEPRPLYNLPDILKADPATPIFVAEGEKCVEALRQCGLL
                     ATTSSGGCNAASKTDWSPLSGRHVTILPDNDEPGRRYAQEVTDILLRLAPPATVRILK
                     LADYAPGLPEGGDVADIVESDGWFGLPLGQGASPEDLGRWLIEIAAGIEPVASKDEWP
                     EVISLDPGELPQFPVHVLPTILRDWVQEEARATQTPLDLPAMVSLGVCSAAIARQVEV
                     VPRVGWIEPVNLFVTVLLPPGNRKSAVFRDALKPLRDYEAELIDMARPEIAKRQSARR
                     QAEARLHKLEKFAGEKDDQEAAEQACELAARLAVEPEPVLPRLLTDDATAEKIASMLA
                     EQKGRLASMSPEGGPFDLMAGLYSKNQTPQFTVYLMAHAGDELIADRVSRKSVRVERP
                     ALTCCYVIQPEVIRSLTHKAAFRGRGLLARFIYAVPVSTIGKREIAPPPVRSEVAEAY
                     HRTVTMLARQANAIGLAPLKLALTTEAELLLRTWEAEIEGMLAEFGEMETIRDWGAKL
                     AGATLRVAAVLHCVEQGIRGEIAPQTLAAAIEIGRYLIPHAKAVLEQLSDDSRLDGAR
                     WILRWIRRHQLREFTLRDVYRHGYRHFDSVDECKAALMELERRGYVREQQLPPQGPSA
                     RNTPLYGVNPVLYTPKGVSPIYGGEHVLEMPKGVDSADNVDTTPTTHDQSVNVVNVVS
                     AFQKSEIVLTPHKCGISNDEPQAGFHPTSGGANRHDGQAPSVPSGPPLAAPSHVVAVY
                     GTQQEGGEVDGPEFEEVTL"
     gene            complement(5489..5704)
                     /locus_tag="PLD05_07780"
     CDS             complement(5489..5704)
                     /locus_tag="PLD05_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOP77376.1"
                     /translation="MTGEREMLSLRETAKVLGLSERSVWTAIKAGRIPSTRIGKRVLI
                     PRRLLLERLEAEARAAMQASNDEGAGR"
     gene            complement(5901..6815)
                     /locus_tag="PLD05_07785"
     CDS             complement(5901..6815)
                     /locus_tag="PLD05_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77377.1"
                     /translation="MSVVERFNRLDRAVEDFQQAAEGFAELFQANEPQYGVYVSNRID
                     PKSGVTWDTMLNRLTDLLEASYKLLEAISDLPQKVQELLRRTEWPLLTMPQRAEEVIR
                     FAKCWIPLLEKRQSWEYYSFYYDFDGYVASFLGSVWEDWPQAMEDYQNRKAKIRRDCL
                     LSGEAAWFGDEAAEKSGATPATGMVSVNEATEKLQKGQTKGNGEAFIDRQEGPAEPEG
                     NMTEADALRSLLALFTQHVSDERFTAMAAILADPNRTIDEKLWEIHRRIPIPPTVDGP
                     TLASIFKTTKQAIYRTTWWQRNRRSSDD"
     gene            complement(7341..7766)
                     /locus_tag="PLD05_07790"
     CDS             complement(7341..7766)
                     /locus_tag="PLD05_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77378.1"
                     /translation="MTIYSLGNPHRAEQDLRTKAVIKGYSPREFAQLVGVAPTYLWQA
                     QQGNIQPARTGPLKGHYSRAKRSCVMALPTHPVWLGILPLWESPVDRATPKCRLRMAG
                     FCVGKLGARSKTRLPVAGPRFFPDLLWPLTPDERPAPVS"
     gene            complement(7971..8093)
                     /locus_tag="PLD05_07795"
     CDS             complement(7971..8093)
                     /locus_tag="PLD05_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77379.1"
                     /translation="MTRRLRVVNDVLQPIRVERGEGACPASLEFLSNRILEALI"
     gene            complement(8090..11401)
                     /locus_tag="PLD05_07800"
     CDS             complement(8090..11401)
                     /locus_tag="PLD05_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77380.1"
                     /translation="MKFDVAAVRNCLKSFDFQTLFREHLGWDNYHAQLDVPVGENTFH
                     LTAVAQKRGFVAFVCPSIPDRATRLKIDHHVTKSVREHFVIYFDKAAGQQVWQWVRRE
                     PGKPLASREYRFHVSQSGDPLIQRLNQIAVSIEEEEQITVVDVANRARAAFDVDKVTK
                     KFYDRFKAEHADFLKLIKGIKYEEDLQWYTSLMLNRLMFVYFIQKKGFLDGDTDYLRN
                     RLKRVQEIKGKGKFHTFYRYFLLRLFHEGLGKSPEERKLDPDFERLLGKVPFLDGGFF
                     EVHELEQRWEEIDIPDEAFEKLFDFFDQYRWHLDERPLRADNEINPDVVGYIFEKYVN
                     QKQMGAYYTKEDITEYISKNTIIPFLFDAARKKCPIAFKPDSAVWRLLREDPDRYIYP
                     AVRHGVIRPDGSVVPESDLPEFVQKGMHDPNARMFDKRYNLEQAPAGDPIRLVTETWR
                     EYVYRRNRCLEIREKLQKGEIHEINDLITLNLDIWQFARDAIVNCEDPELLRAFWHAV
                     EKITVLDPTCGSGAFLFAALRILETLYGDCLERMERFVEDLAGKKHPPEQFSDFKEVL
                     ERVAEHPNERYFILKSIIINNLFGVDIMEEAVEICKLRLFLKLAAQVEKIDQIEPLPD
                     IDFNIRAGNTLVGFASLEQVRKSQEGRLGFAAEEARRIEEEAELVERAFQQFRAQQTT
                     HGGRVTHRDKQQLRRRLEKLTDKLDRFLAGEYGVNLDKPEEFEAWKASHQPFHWFAEF
                     YGIMRNGGFDVIIGNPPYVEYSQRSVGYRVLENEYLTFSSRNLYAFVYERSKHLDHSH
                     ARLGIIVQISSVSTPQMRTMAFEIRHGQNFVWVSNYATRPSCLFEGVTMNLTILLSKT
                     GVAEQDTPNHLWSTKYIRWNPESRHIVFPTITYVDVPPDVYLFDFAVPKLTSACEITM
                     LRRLLRERQTLEALLEISKARTSQCLHYRTAGGRYFKIFSDRPLGTESTSNKRKFLKE
                     EYNARAAIAVLSSSLWWWYYTLHFDMYNCKDYMIYGFRFDFNKSGAAQELAELGSALV
                     ESLFKNAKRKTQQYATTGARRQLLFRPSASKAIIDEIDKVLAKHYGFTDEELDFIINY
                     DIKYRMGDDAGVDEE"
     gene            complement(11419..14823)
                     /locus_tag="PLD05_07805"
     CDS             complement(11419..14823)
                     /locus_tag="PLD05_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOP77381.1"
                     /translation="MPQIFDNIDNYLLSAITQAMGLSQCADFCVGYFNLRGWKVVADL
                     VDQWPGGPGHCCRLLVGMHQPPHDLLKRALNPVKGDEEIDQVTAIRLKRELAQEFRDQ
                     LTLGIPTNEDEAALRRLASQIANQKVVVKLFVRHPLHAKLYLLFRQDPVNPKIGYLGS
                     SNLTLAGLSKQGELNIEVLDPDACDKLAKWFADRWNDRFCLDISKDLVEIINESWARV
                     EPIPPYHIYLKIAYHFSQEARAGLTEFRIPSDFDGKLLEFQKAAVKIAAHHLNKRGGV
                     ILGDVVGLGKTLMATALARIFEDDHGFETLIICPKNLVSMWEDYRQRYRLRAKVLSIT
                     RVQEELHRLPRFRLVIIDESHNLRNRESKRYRAIAEYIASNDSHVILLSATPYNKTYL
                     DLSNQLRLFVDEHKDVGIRPERLLRELGETEFIRRHQCPVRSLAAFEKSEYADDWREL
                     MRLYLVRRTRSFIRENYAKLDAARKRKYLEFEDGTRFYFSTRLPKTVKFEIDLQDPQD
                     QYARLFADEVVRTVNDLTLPRYGLGNYVKPSPHNPPTAEEAKVLADLSRAGTRLKGFC
                     RTNLFKRLESSGHSFILSVQRHILRNYIFLHAIENGLPLPIGTQDMGLLDTSANDEDS
                     QLWDPAADDDENQSPDKSVAIQSQLPNESDLQRRAADLYQTFASQFHRRFKWLRADLF
                     DKALADDLRKDAQALIKAIQRAGTWDPQRDTKLQSLVQLLTKQHPDEKVLVFSQFADT
                     VRYLEEQLKASGLTAVAAVTSATSNPTEIAYRFSPVSNEKREFVNPADELRVLLTTDV
                     LSEGQNLQDCSIIVNYDLPWAIIRLIQRAGRVDRIGQQSDTILCYSFLPADGVERVIR
                     LRARVRQRLRENAEVVGADEAFFEGEADDQPLRDLYTEKAGILDGEEDTEVDLASYAY
                     QIWKNAIDRNPQLEKIIPAMPNVVYSAKPLPPNADRREGVLVYVRTGEGNDALTWIDT
                     DGNSVTESQFAILKAAECTPDTPALPRAEDHHKLVEKAIELIVKTEKSVGGQLGRPSG
                     ARFRTYERLKAYAQAIKGTLLESADLLKAIEDIYKYPLLQSAIDTLNRQLKSGISDED
                     LAALVIRLRQEGRLCEVAKDGEAGRPQLICSLGLRRPDSHAARSCV"
     gene            complement(14892..16199)
                     /locus_tag="PLD05_07810"
     CDS             complement(14892..16199)
                     /locus_tag="PLD05_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOP77382.1"
                     /translation="MRGTDVVNDRRLIEDYLPIEAISREASREKSVRKGHISTLHLWW
                     ARRPLVACRAAVYAALVPADRWVKEVNLKNPPPHPEKAERMKNGAKLGLNRGAAAEFV
                     TRLCKYPGDPKVIAEAQRHILEAHAERLSVEIGKPVTVEDIVEGRAPRPKVLDMFAGG
                     GAIPLEALRLGCEAYALDLNPVAHIIELCTLVYPQKYGKPDPNTRGMTRPGQAGGLSS
                     TWGGLAKEVRYWGNWVLQKVKAEIGDLYPLIPERGRTGVPPVPPVPPVIQGELFQTRR
                     HLPHWQIGGSVYFITFSTKKIELPEVCRMTILDACRFWDGKKIRLHLVCVMPDHVHAL
                     IEPLEETPGVYHDLSKVLHSIKSFTAHEINKMMRRSGPLWEEESWDRIIRDEKDYYEK
                     WQYIVNNPVKAGLSASPEQYAFLYANEKLAWEITGPAGTGDNT"
     gene            complement(16196..16744)
                     /locus_tag="PLD05_07815"
     CDS             complement(16196..16744)
                     /locus_tag="PLD05_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77383.1"
                     /translation="MDRLKKAALLTRLIKRLREEESWCGETHVQKATFFLQRLMQVPL
                     GFDFILYKHGPFSFDLRDELTALRADDLVRLEPQGPYGPRIAATERSKYIEELYSKTL
                     AEYGGRIDFVAKSLGRKGVADLERLATALYITELAGSGASVNERANQMTALKPHIPPE
                     IARDAIEQVDRIIREAQRYLPA"
     gene            complement(16737..18071)
                     /locus_tag="PLD05_07820"
     CDS             complement(16737..18071)
                     /locus_tag="PLD05_07820"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP77384.1"
                     /translation="MSRNFHEIRDPIHAFVQMDNDEREVVNSRPFQRLRHVHQLALTY
                     LIYPAATHKRFEHSLGVMELASRVYDVVTHPDNVTEKMKDRLRPLTDSDKLRYWRRTL
                     RMAALCHDIGHLPFSHAAEKELLPEEWDHERLTRELIASEEMQEIWNRITPPLRVEDV
                     VKLAVGPKKAKHLTFNDWETILSEIIVGDAFGVDRMDYLLRDSHHIGVAYGKFDHYRL
                     IDTLRILPMPSCGDDNESSEELALGVEEGGIHSAEALLLARYFMYSQVYCHPVRRIYD
                     IHLTDFLREWLPDARFPTDLQQHLRMTDNEVTAALLDAAHDQKKRGHVHARRIVARDH
                     FKLIYQRNPSDVEINPEAGKAIFEALCDKFGSENFRHDRYHQKSGSSDFPVKMRDGRI
                     VSSLAISQALNTIPVVSVDYVFAERAVFNEAEKYLRQHRDKIIRPKKEEDNG"
     gene            complement(18074..18331)
                     /locus_tag="PLD05_07825"
     CDS             complement(18074..18331)
                     /locus_tag="PLD05_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HOP77385.1"
                     /translation="MKRIEVKGRRRGQAVRLTTNEWYMAQQLGETYWLYVVWDPLGPK
                     PELVRIQNPAVRLDHAKREIVAARFFEIPAEAVVRAAERKD"
     gene            complement(18354..18506)
                     /locus_tag="PLD05_07830"
     CDS             complement(18354..18506)
                     /locus_tag="PLD05_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77386.1"
                     /translation="MSARFAEIGKVNDRRLIEDYLPIEAISREASREKAMRKGQISRV
                     LRAPDR"
     gene            18785..19015
                     /locus_tag="PLD05_07835"
     CDS             18785..19015
                     /locus_tag="PLD05_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77387.1"
                     /translation="MRILLSASVSVLVHGVLALVKPAGPPPQGTNNRHLEQGDLACQA
                     PPAAQEFARILGFAGRAIGLDRAARDAYSPAL"
     gene            19012..19404
                     /locus_tag="PLD05_07840"
     CDS             19012..19404
                     /locus_tag="PLD05_07840"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HOP77388.1"
                     /translation="MKLWEPKCDSDHDRLVEWMAKLEEELFAVMDRNAELKEENARLR
                     GEVADLKQQLAAARKNSSTSSKPPSSDIVKPEKPLPKGGKKRSKDGQPGHEQHLRSPF
                     PPKGGQPLAALHARLLPGLRPQAGRFAL"
     gene            19480..20265
                     /locus_tag="PLD05_07845"
     CDS             19480..20265
                     /locus_tag="PLD05_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOP77389.1"
                     /translation="MPALPQGALRPAARAHGVSGLFGPRLTALVAFAKAVCQASFLTI
                     RTFLPDVVRIYVSRGYLAKLIGKVSESLAPSYAELFQRLPGESVLNIDETGHKENGAR
                     FWTSCFRAQMYTFFRIDKCRGSKVLLEVLGEEFNGVPGCDYFSAYRKYMRDCDVLVQF
                     CMAHLIRDVKFLLTLPGREVQAYGEPLRDALRDLFGVIHRREKMSAAGFQKALEAARE
                     QVIKAATTRVPEAKHARNLPARFRVIGEAYFRFITTLAIEDDE"
     gene            complement(20574..>21243)
                     /locus_tag="PLD05_07850"
     CDS             complement(20574..>21243)
                     /locus_tag="PLD05_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77390.1"
                     /translation="ELQPTFLEQAPLEAAPGGTPESRMKPAWVALVKILEEIENERYH
                     YPVGRTSFQKIAYFATESGIPTGLSYRRSSYGPYAPELKGLITRLVNNGLIREERLGR
                     MFAVKPGPTFKDAWRAYERDLADWKDTIEALADLFMRMRTEQAELAATVHFAAKGLAL
                     KKKSEPTEREVLSEVMSWKQKRRPALKEEDVAVTIRGLSMLGWLKVKPSSDLPVSEEA
                     VLDV"
BASE COUNT         4196 a         6222 c         6031 g         4794 t
ORIGIN      
        1 gatagagcag cggatttcta atccgctggt tgggggttcg aatccctccg ggcgtgcttt
       61 gtataatgtc gcaactcgtt gcagcgtctt gatttacaaa tagtgtctat ttgttctatc
      121 acaattgcac ttcatcgtct tgcaatgcat ttcttgcagg ttctgcgttg cattttgcgt
      181 tgcgttttgc gggggtgttt ttcaaactgt gctgatcgca aaagcggcaa actatgctga
      241 tcgcaaaagc gctaaactgt gctcatcgcc gaagccgtca aactatgctg atcgcaaaag
      301 ccgtcaaact gtgttcatgg ccgaagccgt caaactgtgc tcatcgcaaa agcgccaaac
      361 tatgctgatc gccaaagcgc taaactgtgc tcatcgcaag agcgtcaaac tgtgctcatc
      421 gccaaagccg tcaaactgtg gtcatcgcaa aagcgctaaa ctgcggttat cgccaaagcc
      481 gtcttgggtg gccttgcgaa ccaagtcatc agggacacgc tggtagtgct tcaagccaaa
      541 cttccccctg ctaactgggg ggatgatgac tgtaagtgat ttcaagacaa tgatttgcgc
      601 gcattaagca ccctgattcc cccaccgttt tctgtgtaca cccacgtggt ccgtggaatc
      661 taccatcagg aagcaccctg attccgcatc gttttctgtg tacagctaag tcgagtcccc
      721 atcggcccag ctcagggcac cctgattccc ccaccgtttt ctgcgtacgg cgcacatcgc
      781 gcaactcaaa gcaggacaag aacttacgca aaaccatcgc atgactacag tatgcaacac
      841 aaatcaacct gaactcgtta cccagcaatg acttacgtaa aaatacatcc aggaactttg
      901 ggctaaactg tgctcatcgc aaaagcgcca aactatgctg atcgcaaaag ccgtcaatct
      961 gtggtcatcg caaaagcgtc aaactgtggt catcgcaaaa gcggcaaact gtgctcatcg
     1021 caaaagcgtc aaactatgct gatcgccaaa gcgctaaact gtgctcatcg caagagcgtc
     1081 aaactgtgct catcgccaaa gccgtcaaac tgtggtcatc gcaaaagcgc taaactgcgg
     1141 ttatcgccaa agccgtcttg ggtggccttg cgaaccaagt catcaggcac acgctggtag
     1201 tgcttcaagc taaccacggg gccatggcca agccactggg cgactgcgtt aatcgggtag
     1261 gaacgggcaa agtcggtggc tgccgaacgc cgtaggcaat ggaacgggtc acgccacggg
     1321 gttaagccgg agcgttcgat aatcctccgc agcgtccggt gacgattcgc cttggggacg
     1381 gtgacgatca gcgattcacc ctcgtgggcc gcaaagtaag cctgctggag tagctctcga
     1441 agtcgtggga caatcggcac gagccgggtt cgtttcgtct tgggggcaaa caccttaagc
     1501 gtcccagtct cctaattgat gtctgcccaa gtgatggagt cggtttcgct tggggaacgc
     1561 aagcccgcaa gtcgggccag cccgagtagc acacgccagc cgtgattggg gcacgcatcc
     1621 agtagccgct cgaagtcttc gagcgagacg tatcgttcat cgttgcgggc catcgacgcc
     1681 ccgacaagcg tttggaatgg gttattgtcg gccagccggt gcgtcattgc ccaattgaac
     1741 aatcccttgg cgttgcgaac gtgaagtcgg acggtcgctt ccgtgcgtcc ttcggcaagc
     1801 cacgtccgcc atcgttccgc gtcctcttgg gtgatcgccg acagccgggc cttggggccg
     1861 aagtaaccgc tcagtagctt ggttgtgttt ttgattcgcg actgcgatct caaagccaag
     1921 ccagcaacgt attcccgttc gtagcgttcg atcaggcgtc cgagggtttc gcggactcgt
     1981 gcgggggcta gttggcaacg ttcaagcttc tcataaaggc cgtccggcaa ggctgccagc
     2041 cacaccgccg ttgggccggg aatcgccgtg gcggtggcca tactggcggc caattgctcc
     2101 acgtggacct tgaagctctc ggcttgccga cgtggtagct tgccaagata gagcgtcacg
     2161 cgccggccat cgggcaggca aacatacagt cggaatgtat ggccatgctt gcttagtgat
     2221 gccatgtcag cccctccctt tgtcagcttt gttcccggta attgtggcga caccttttgt
     2281 ttcacacaac acttggtcgt tggagtccac aaactgaatt ggccaggcct cgcggctgtt
     2341 gaagtcctcg gggtggattc tccctagtcg gcacatatcg aggagcaagg caacggcttg
     2401 gtcgtgaaat gtgtcgtaca gctcgaataa cgaggggaag tcaacgccgg ggtgagcttg
     2461 cccgcggggg acgataaaat agccctcacc atcgctccgc cgctggagat gcagcgtata
     2521 gcccgcttgc caaagcttgt ccaggagcaa ccgtccgccg acaaggggct tattctgtcg
     2581 gagggtttcg atttcgtcct ttgtcaaacc gcccttgacg aaacgcaact tcaattggcc
     2641 gttagcccaa tcgacgctca caccgggccg cttaagtaat gcgatcggat tcatagggtt
     2701 acttcctcga actcggggcc gtccacttcg cccccctctt gttgcgtacc ataaaccgcc
     2761 acgacgtggg acggggctgc caggggcgga ccgctcggca ccgagggggc ttgcccgtcg
     2821 tgcctattag ccccgccact tgtggggtga aaaccggctt ggggttcgtc gttcgatatt
     2881 ccacacttat ggggggtgag cacgatttca gacttctgaa atgcgctgac aacgttgaca
     2941 acgttgacag attggtcgtg cgtcgttggg gttgtgtcaa cattgtcagc gctgtcaacg
     3001 ccttttggca tctccaaaac gtgctcgcca ccataaatgg gggaaactcc ttttggcgtg
     3061 tacaagacgg ggttgacacc atacaggggg gtattgcggg ctgaaggccc ctgggggggc
     3121 aattgttgtt cacggacgta cccacgccgc tctagctcca tgagggcggc cttacactcg
     3181 tccacggaat caaagtgacg gtagccgtgg cgataaacat cgcggagcgt aaactcgcgt
     3241 aattgatgcc gccggatcca gcgcaaaatc caccgtgcgc cgtccaaccg ggaatcgtcg
     3301 cttagctgtt cgagcaccgc cttggcatgc ggaatcaggt accgcccgat ctcgattgca
     3361 gcagccaggg tttgtggggc aatctcgcca cgaatgccct gctcgacaca atgaagcaca
     3421 gccgccacgc gcagcgttgc accggctagc ttggcacccc aatcgcggat ggtttccatt
     3481 tcgccgaact cggcaagcat gccctcaatt tcagcttccc aagtccgcag caacagctcg
     3541 gcttccgtgg tcagtgctag cttcaatggg gcaaggccga tggcgttggc ctgcctcgct
     3601 agcattgtca cagtgcggtg ataggcttcg gccacctcgg accgcacagg gggaggggct
     3661 atctcccgct tcccgatcgt gctcaccggc accgcataaa tgaaccttgc caataacccc
     3721 cggccacgaa acgccgcttt gtgagtcagg ctgcgaatga cctcgggttg tatgacgtag
     3781 cagcacgtca gcgctggccg ctccacgcga acactcttgc gagaaacgcg atcagcgatc
     3841 aactcatcgc ccgcatgggc catcagatag accgtgaact ggggtgtttg gtttttgctg
     3901 tacagcccgg ccatcaggtc aaatgggcca ccctcgggcg acatactggc taaccgcccc
     3961 ttttgctccg ccagcatgga agcgatcttt tcggctgtcg catcgtcggt gagaagtcgg
     4021 ggcagcactg gctccggctc aacagccagc cgggccgcca gttcacacgc ttgctcggcc
     4081 gcttcctggt catctttctc gccagcgaat ttttccagtt tgtgcaaccg cgcttcggcc
     4141 tgccttcgcg cagattgccg ctttgcgatt tcgggccggg ccatgtcgat gagctcggcc
     4201 tcgtaatcac gcaacggttt gagagcatct cgaaaaacgg ccgacttgcg attgccaggt
     4261 ggcagcagca ccgtgacaaa aaggttgacc ggctctatcc atccaacacg agggacgact
     4321 tccacttggc gtgcaatggc cgccgagcat acgcccaacg acaccatcgc cggaagatcc
     4381 agcggcgttt gtgttgctcg cgcctcttcc tggacccaat cacgcaatat tgtgggcagg
     4441 acgtggacag ggaactgggg cagctcccca gggtcgagac ttatcacctc gggccattcg
     4501 tctttgctcg cgaccggctc gatgccagcc gctatttcga tcagccaacg cccaaggtcc
     4561 tcggggctcg cgccctggcc gaggggtagc ccaaaccaac cgtcggattc cacgatgtcg
     4621 gctacgtcgc caccctcggg tagtcccggg gcgtagtcgg ccagcttgag aatccgcacc
     4681 gtggccggtg gcgcaagcct caaaaggatg tccgtcactt cctgggcgta tcgccgcccg
     4741 ggttcgtcgt tgtccggcaa gatggtaaca tgtcggccac ttagagggct ccagtcggtt
     4801 ttgcttgccg cgttgcagcc gccgctggat gtcgtggcca gcaagccgca ttgccgcagt
     4861 gcctccacgc atttctcccc ttcggcaacg aaaattggtg ttgcggggtc ggctttgaga
     4921 atgtcgggca ggttgtacag gggacgtggc tcgggcatgg ccgcaattcg ccagccatcg
     4981 ggaaaccgac tgattgggcg gatgtccttt gtcccgtcgg gtttgtccca acgcaccacc
     5041 agcccgaccg gctcccctgc ggcgtcgcaa taggtccacc ggcaggattc ttggcctagc
     5101 tttcgccgca atgaatcaag cgcatcctcg gccgcaggaa acgtttgacc gtcgggttta
     5161 ccgttgccgc ccggtcgcgc atcagcacaa cctacgtatt tcgcccgttt tacggggttt
     5221 tggtgatgtg gcgatgtggt gacatggtga gattgcaaca cgtcatcggg catcaagtct
     5281 ttcatcgcca accccagggc cgcgacaatg ctttccgtgg cacacccggc atgacagcaa
     5341 accaaaaccc ggccgtccgt tccttcggta accgaaaggc tcggtcggcg gtcatcatgg
     5401 gccgggcatt gggccgacca gccggttccc gaccgcttcg gacggcatcc gtgccgatca
     5461 agggccgtga gaacggtgtc aatgggcttc atcggcctgc cccctcatcg ttgctggctt
     5521 gcattgccgc ccgggcctcg gcttccagcc gttccagcag cagccgccgc gggattaaaa
     5581 ctcgcttgcc aatccgcgtg ctggggattc gtccggcttt aatcgccgtc cacacagaac
     5641 gctcagacaa gcctaaaacc tttgcggtct cacgtaacga cagcatttca cgttcaccgg
     5701 tcatgtttgg tcctttcatg gagcaggtgg tgcataaggg tttttcgact ttctaccctt
     5761 gagggcgccg ggtaacggtt taggtaatcg caagcgcatg aaaaaagccg cagacagcgt
     5821 ttttctgcgg ctttgcaacg tccaaatttt ttgagtcggg taactgctgg gtaactttgg
     5881 gtaactgtta cccgccaagt tcagtcgtca gacgagcgcc gattcctttg ccaccacgtg
     5941 gtgcggtaaa tggcttgttt ggtcgtcttg aatatcgatg ccaatgtggg gccgtcaact
     6001 gtcggcggaa ttggtattcg ccggtgaatt tcccacaatt tctcgtcaat ggtcctgttt
     6061 ggatcagcca agatggccgc catggccgta aaacgctcgt cgctcacgtg ttgtgtgaaa
     6121 agggccagaa ggcttcgcag ggcgtcggcc tcggtcatat tcccctccgg ctccgcggga
     6181 ccctcctgac gatcaataaa tgcctcccca ttacccttgg tttgcccttt ttgcagcttt
     6241 tccgttgctt cgttcacaga aaccataccc gtcgccgggg ttgcgccgct cttttctgcc
     6301 gcctcgtcgc caaaccacgc tgcttcccca cttagtaggc aatcgcgacg gatcttcgcc
     6361 ttgcggtttt gataatcctc catcgcctgg ggccagtctt cccagacgct tcccaaaaaa
     6421 ctcgcgacat agccgtcgaa atcgtagtag aacgagtaat actcccagga ttgtcgcttt
     6481 tccaggagag ggatccagca cttggcaaag cggatcactt cctcggccct ctggggcata
     6541 gtcagtaacg gccactcggt ccgacgcagg agctcttgga ccttctgggg caggtcgctg
     6601 atcgcctcga gaagcttgta cgacgcctcc agcaggtccg tcaggcgatt aagcatagta
     6661 tcccacgtga ctccgctttt ggggtcgatc ctgttgctca cgtatacccc gtattgcggt
     6721 tcgttggctt ggaacagctc ggcaaagcct tccgccgcct gctgaaaatc ttccaccgcc
     6781 cgatcaagcc gattaaaccg ctccaccaca gacatgcgtt gccctcgcac aacgtgaccg
     6841 cccaataaga caaagggcgg gcagccgccg ggcaatagcg gagttcggtt ggcctaacct
     6901 agcccgccct tttttacccc agtattttta gcccacttca cccgggtttc acaccccagg
     6961 ttggacaagg gttgtccaac ctgcatcatt tcccccagcg ctagggcgtc gggaaggttg
     7021 cctattttct ccggggcgcg ctgtgttttg ttgcgatcta gcaaaatggg taaacttcca
     7081 ctgcgacccg tcactccgca ctggcctgtc tctcgccgcg cccagtcacc cggcattggc
     7141 gtgtccctcg ccgcgcccag tcacccggca ttggcctgtc tctcgccgcg cccagtcacc
     7201 cggcactggc ctgtctctcc atgtgtttcg cgcgcaccgc caagcgtttt ttgctgggta
     7261 caacccagtg tttccccggg agcgctcatt cgcccgtccc gctagcttcg cgccgaactg
     7321 agctatagag agaaagaaag tcagctcacg ggggctggcc gttcgtcggg ggtcaacggc
     7381 caaagaaggt cggggaagaa cctaggaccg gcaaccggca ggcgagtttt tgatctggca
     7441 cctagcttcc cgacgcaaaa cccggccatc cgcaagcggc atttcggggt tgctcgatca
     7501 acagggcttt cccaaagcgg gaggattcca agccagaccg ggtgcgtcgg caaagccatg
     7561 acgcagctcc ttttcgcacg ggagtaatgc cctttgagcg gaccggtcct cgcaggttgg
     7621 atattccctt gttgtgcctg ccacagatac gttggagcga cacccaccaa ctgggcaaat
     7681 tcccgcgggc tgtagccctt gatgacggcc ttcgtgcgga ggtcttgctc cgcccggtgc
     7741 ggattcccca gggaataaat tgtcataatc ctacgaattc cctgaaaatc gtttgctgtt
     7801 tctgatcgtc ggtggcaact ttctcaagcc actcagcgac aacggacaac cggccgtcca
     7861 cttcgagaag atcgctcggc ccataggcct cgccattggg gtgcactagg gctggcggga
     7921 atatagccgg ggacgtggcc agagtttttt cggattgacg taagttgttt tcagataaga
     7981 gcttctagaa tccggttcga gaggaattct agcgaagccg ggcatgcgcc ctcgccccgt
     8041 tccacgcgga ttggctggag gacatcgttg accacacgca gccgcctcgt cattcctcgt
     8101 ccaccccggc gtcgtccccc atgcggtatt tgatgtcgta gttgatgatg aaatccaact
     8161 cttcgtcggt gaacccgtag tgctttgcca ggaccttgtc gatctcgtcg atgatcgcct
     8221 tcgatgccga aggacgaaag agcaactgcc tccgcgcccc tgttgtggcg tactgttgtg
     8281 tctttctttt tgcatttttg aaaagcgatt caacgagtgc acttcccaat tccgccagct
     8341 cttgtgccgc gccactcttg ttgaaatcaa agcgaaaacc gtaaatcatg tagtctttgc
     8401 agttgtacat gtcgaagtga agggtatagt accaccacca cagactagag cttagaaccg
     8461 caattgccgc tcttgcgtta tactcctctt tgaggaattt ccttttgttg gaagtacttt
     8521 cggtacccaa agggcgatcc gaaaaaatct tgaagtagcg cccaccggca gtccgatagt
     8581 gcaggcactg cgaagtgcga gcctttgaga tctcaagtaa ggcctcgagg gtctgccgct
     8641 cgcgcaagag ccgacgaagc attgtgatct cacaagcgct ggtgagtttt gggaccgcaa
     8701 aatcgaacaa gtacacatcc ggagggacgt cgacataagt tatcgtggga aatacaatgt
     8761 gtcggctttc gggattccat ctgatgtact tagtgctcca taaatggttt ggcgtatctt
     8821 gctcggcgac tccagttttc gaaagcagga tggttagatt catcgtgacg ccttcaaaca
     8881 agcaggatgg ccgagttgcg tagttggaca cccacacaaa gttttgaccg tgccttattt
     8941 cgaatgccat tgtacgcatc tgaggagtcg atactgagct gatttgcacg attattccaa
     9001 gccgagcgtg tgagtgatcc aaatgtttcg accgttcata tacgaaggca tacagatttc
     9061 tgcttgagaa ggtaaggtac tcgttctcga gaacccggta tccaaccgat cgttgcgagt
     9121 actccacata cggcggattc ccgatgatca catcgaagcc gccattgcgc ataatgccgt
     9181 agaactcggc gaaccagtgg aagggctggt ggctggcctt ccaggcttcg aactcctcag
     9241 gcttgtccaa gttgacgccg tactccccgg ccaggaaacg gtcgagcttg tccgtcagct
     9301 tctccaagcg acgccgcaac tgttgcttgt cacgatgggt gacccggccg ccatgcgtcg
     9361 tctgctgggc gcggaactgc tggaacgccc gttcgaccag ttcggcctcc tcctcgatcc
     9421 gtcttgcctc ctccgcggcg aagccgagcc tgccctcctg ggacttgcga acctgctcaa
     9481 gggatgcaaa cccaaccagc gtgttcccgg cgcggatgtt gaaatcaata tccgggagag
     9541 gctcgatctg gtcaatcttc tcgacctggg ctgccagctt aaggaacagc cgcagtttgc
     9601 agatctcgac cgcctcctcc atgatatcga cgccgaaaag gttattaatg atgatggact
     9661 tgaggataaa gtagcgctcg ttggggtgct cagcaacccg ctctaggacc tccttgaagt
     9721 cactgaactg ctcgggaggg tgcttcttac cagcgaggtc ctccacgaag cgttccatcc
     9781 gctccagaca gtctccgtag agggtctcca gaattcgcag ggcggcaaag aggaacgcgc
     9841 cggaaccgca ggtcgggtcg aggacggtaa tcttctcgac ggcgtgccag aacgcccgga
     9901 gaagctccgg gtcttcgcag ttcacgattg cgtcccgggc aaactgccag atgtcgaggt
     9961 tgagcgtaat caggtcgttg atctcgtgaa tttccccctt ctgaagtttc tcccggattt
    10021 ctaaacaacg attccggcga tagacgtact cgcgccacgt ctcggtgacg agacggatcg
    10081 gatccccggc gggcgcttgc tcaaggttat accgcttgtc gaacatgcga gcattggggt
    10141 catgcatgcc tttttggacg aactcgggca ggtcactttc tggcacaacc gatccgtcgg
    10201 gccgaatgac gccatgacga acggccgggt agatgtaacg gtcgggatcc tcgcggagca
    10261 gtcgccagac ggctgaatcg ggcttgaagg cgatagggca cttctttctg gcggcgtcga
    10321 acaggaaggg gataatggtg ttcttgctga tgtactcggt gatatcctcc ttggtgtagt
    10381 aggcccccat ctgcttctgg ttgacatact tctcgaaaat gtagccgacc acgtcggggt
    10441 tgatctcgtt gtcggcccgc aggggacgct cgtcgaggtg ccaacggtac tgatcgaaga
    10501 aatcaaatag cttttcaaac gcttcgtcgg gaatgtcaat ctcttcccac cgttgttcga
    10561 gttcgtgtac ctcaaagaat ccgccgtcca gaaatggcac cttgccgagc agcctctcaa
    10621 agtcgggatc cagcttgcgc tcctccggag acttgccgag gccctcatgg aagaggcgta
    10681 gcaggaagta gcggtagaac gtgtgaaact tgcccttccc cttgatctcc tgcaccctct
    10741 tgaggcggtt gcggagatag tccgtatcgc cgtccagaaa tcctttcttt tggatgaagt
    10801 acacgaacat caggcggttg agcatgaggg acgtgtacca ctgaagatcc tcctcgtatt
    10861 tgattccctt aatgagcttg aggaagtccg cgtgttcggc cttgaaccgg tcgtagaact
    10921 ttttggtaac cttgtccacg tcgaacgcgg cccgggcgcg gttggctacg tccacgacgg
    10981 tgatttgttc ctcctcctct atgctcaccg cgatctggtt gagtcgctgg attagggggt
    11041 cgcccgactg agagacatgg aaccgatact cccggctggc caggggcttc cccggttccc
    11101 ggcggaccca ctgccaaacc tgttgtccgg ctgccttgtc gaaatagatg acaaagtgct
    11161 cgcggacaga tttggtcacg tggtggtcga tcttgaggcg tgtggcgcgg tcggggatgg
    11221 agggacagac gaacgcgaca aaacctcgct tctgtgcgac cgcggtgagg tggaacgtat
    11281 tctcgccaac gggaacgtcg agttgggcat gatagttatc ccaccccagg tgttcccgga
    11341 aaagagtttg gaagtcaaag gactttaggc agttcctgac ggcagcaaca tcgaatttca
    11401 tgagcttgtc ctccgcactt acacacacga ccgggcggca tgactgtccg gtctgcgtag
    11461 tccgagggaa caaatgagtt gaggccttcc ggcttctccg tcctttgcca cctcgcacaa
    11521 gcggccctcc tgtcgcaacc tgatgacaag ggctgccaaa tcctcgtcgg agatgccgct
    11581 cttgagctgg cggttgagcg tatcgatcgc ggattggaga agggggtatt tgtagatgtc
    11641 ctcaatggcc tttagcagat cggcggactc caagagcgtt cccttgatgg cctgggcata
    11701 ggccttgagg cgctcgtagg tgcggaaccg cgcacccgaa gggcgcccca actggccacc
    11761 gacggacttc tcggttttaa cgatgagctc gattgccttt tcaaccagtt tgtgatggtc
    11821 ctcggcccgt ggcagagctg gggtgtccgg cgtgcattcg gcggccttga gaatggcgaa
    11881 ctgcgattcc gttacgctat tcccgtccgt gtcaatccag gtcagtgcgt cattcccttc
    11941 cccggtccgg acgtagacca gcactccctc gcgacggtca gcgttcggtg gaagcggctt
    12001 ggcggaatag accacgttgg gcatggccgg gatgattttc tcgagttgcg ggtttctgtc
    12061 gatagcgttc ttccagattt ggtaggcgta ggatgcaagg tcaacttccg tgtcctcctc
    12121 gccatctagg atgccggcct tctcggtgta gagatcgcgc aggggctgat cgtctgcctc
    12181 tccctcgaaa aaggcctcgt cggcaccgac cacctccgcg ttctcgcgaa gccgttgacg
    12241 tactcgtgcc cggaggcgaa tgactcgttc cacgccgtcc gcggggagaa aggagtagca
    12301 cagaatcgta tctgactgct ggccgattcg atctacacgc ccagcccgct ggatcagccg
    12361 gataatggcc caagggaggt cgtagttgac gatgatggag cagtcctgca ggttctgccc
    12421 ctcactcaaa acgtctgtcg tcaatagaac gcgcagttcg tcagccgggt tgacaaactc
    12481 ccgtttctcg ttgctgacag ggctgaatcg gtacgcaatc tccgtgggat tggatgttgc
    12541 gctcgtcacg gctgcgacgg ccgtcagacc acttgccttt agctgctcct ctaggtacct
    12601 gaccgtgtcg gcgaattgtg agaaaacgag taccttttcg tcggggtgct gcttggtgag
    12661 aagctggacg agggactgca atttggtgtc ccgctgggga tcccatgtcc cggcacgttg
    12721 gatcgccttt attagcgcct gggcatcctt gcgtaagtcg tcggcgaggg ctttatcgaa
    12781 gagatccgcg cgcaaccact tgaagcggcg atggaactgg gatgcgaaag tttggtagag
    12841 atccgcggcc cgcctctgca ggtcactctc atttggaagc tggctttgta tcgcaacgga
    12901 tttatctggc gattggttct catcatcgtc ggcagccggg tcccaaagtt ggctgtcctc
    12961 gtcattcgcc gaggtgtcaa gcagccccat gtcctgcgtc ccgatgggga gcggcaggcc
    13021 gttttcaatg gcatgcagga agatgtagtt tcggaggatg tggcgttgca ccgacaggat
    13081 gaacgagtgg ccgctgcttt ccaggcgttt gaacaagttg gtacggcaaa agcccttgag
    13141 gcgcgtccca gcgcgagaga ggtcggccag caccttcgcc tcttccgctg taggaggatt
    13201 gtggggcgaa ggcttcacgt agttccctag tccatagcgc ggcaaggtca ggtcgttcac
    13261 cgttctcacg acctcgtccg cgaataggcg cgcatactgg tcttgtgggt cctgtaggtc
    13321 gatctcgaac ttgaccgtct taggcaggcg cgtcgagaag taaaatcgcg tgccgtcctc
    13381 gaactcgaga tactttcgtt tccgagccgc gtcgagtttg gcataattct cccggatgaa
    13441 gctccgggta cggcgaacca gatacaatcg cattagctcc cgccagtcgt cagcatactc
    13501 acttttctcg aaggcagcca gcgaccggac agggcattgg tgtcggcgga tgaattcggt
    13561 ctcccccagt tccctaagaa gtctctcggg ccggatccca acgtctttgt gttcgtcaac
    13621 gaacagccgg agctggttcg agaggtcgag ataggtcttg ttgtacggcg tggccgacaa
    13681 gaggatgacg tggctgtcgt tcgaggcgat atactccgcg atggcccggt agcgtttgct
    13741 ttcgcggtta cggaggttgt gactctcgtc gatgatgacc aggcggaagc ggggcagcct
    13801 gtgaagttcc tcttggaccc gcgtgatgga aagcactttg gcccggaggc ggtatcgctg
    13861 ccggtagtcc tcccacatgg aaaccaggtt ctttgggcag atgatcagtg tctcgaagcc
    13921 atgatcgtcc tcgaaaattc gtgccaaggc cgtggccatt agcgtctttc ccaaacccac
    13981 gacatcaccg aggatcacac ctccgcgttt gttgagatga tgcgcggcga tcttcacggc
    14041 ggccttctgg aactcaagca acttgccgtc gaagtcgctc gggatgcgga actctgtgag
    14101 ccctgcccga gcctcctggg aaaagtgata cgcgatcttg aggtaaatgt ggtacggagg
    14161 gatcggctcc acgcgtgccc agctctcgtt aatgatttcg acgaggtcct tggagatgtc
    14221 aaggcagaac cggtcgttcc aacgatccgc gaaccatttg gcgagcttat cacaggcgtc
    14281 ggggtcgagc acctcgatat tgagctcgcc ttgtttggac agccccgcca gtgtcaggtt
    14341 gctgctcccg aggtagccaa tcttaggatt caccgggtcc tgccggaaca gaaggtagag
    14401 cttagcgtgg agtgggtgac gcacaaacag cttgaccacc accttctggt tggcgatttg
    14461 ggaggctaga cgccgcagcg ccgcttcgtc ctcgttcgtt ggaattccca gagtgagctg
    14521 gtcgcgaaac tcttgggcga gttcgcgctt caacctgata gcagtgacct gatcgatctc
    14581 ctcgtcgcct ttcacagggt tcaacgcccg cttgagaagg tcgtggggcg gctgatgcat
    14641 gccgaccagc agccgacagc aatggccggg gcctcctggc cattggtcaa cgaggtcggc
    14701 gaccactttc catccacgaa ggttgaaata tccgacgcaa aaatctgcgc attgggacag
    14761 ccccatagct tgggttatcg cggacaaaag gtagttgtca atattgtcga atatttgcgg
    14821 catggattac cccaataacc cccagttcaa ggcccaaatt atcccatcat tcatggaagc
    14881 tgctcacgtt cctaagtatt gtcgcctgtc ccagctggcc ctgttatttc ccaggcaagc
    14941 ttttcgtttg catagaggaa ggcatattgt tccggactag cagataatcc ggccttgacc
    15001 ggattattca caatgtattg ccatttctcg taataatctt tctcgtctcg tataatccga
    15061 tcccaactct cttcttccca caacgggccg gaacggcgca tcattttgtt tatttcatgg
    15121 gcagtaaagc tcttgatgct gtgaagaact ttggagaggt catggtaaac gccgggcgtt
    15181 tcctctaaag gttcaatcag ggcatggaca tgatccggca tgacacatac gaggtggagt
    15241 ctgatttttt taccgtccca gaagcgacag gcatccagga ttgtcattcg gcagacttct
    15301 ggcaattcga ttttcttcgt tgaaaaggta atgaaataaa cggaaccccc gatttgccag
    15361 tgaggaagat gtctgcgtgt ctggaacaac tctccctgaa tgacaggcgg cacaggcggg
    15421 acaggcggga cgcctgtcct ccctctttca ggaatcagcg ggtagagatc gcctatctct
    15481 gccttcacct tttgcagcac ccagttgccc cagtagcgga cttccttggc caagccaccc
    15541 caggtggatg acaggcctcc ggcctgtcct ggccgggtca tacctctcgt gttcgggtcg
    15601 ggcttgccgt acttctgggg atagacgagc gtgcagagct cgatgatgtg ggccacgggg
    15661 ttgaggtcga gggcgtaggc ctcgcagccg agccgcagcg cctccagcgg gatggcaccg
    15721 cctccggcga acatgtcgag caccttgggc cggggcgcgc gcccctcgac gatgtcctcc
    15781 accgtgaccg gtttgccgat ttccaccgaa agccgttccg cgtgggcctc caggatgtgt
    15841 cgctgcgcct cggcgatcac cttcgggtcg cctggatact tgcacaagcg cgtgacgaac
    15901 tcagccgctg ccccgcgatt gaggcccagc ttggcaccgt tcttcatccg ctcggccttc
    15961 tctgggtggg gtggcgggtt cttgaggttc acctccttga cccagcgatc ggccggcacc
    16021 agggccgcgt acaccgctgc ccggcaggcc acgagcggtc gccgcgccca ccagaggtgc
    16081 agcgtcgaga tgtgcccctt gcggacggac ttctcccgcg aggcctctct gctaatggcc
    16141 tcgatgggca ggtagtcttc aatgagacgg cggtcgttca caacatcggt ccctctcatg
    16201 cgggcaagta cctctgggct tccctaatga ttcgatcaac ctgctcgatt gcgtctctgg
    16261 cgatctctgg tggtatgtgt ggcttgagag ccgtcatctg attggccctc tcgtttaccg
    16321 aagcgccact gcccgcaagc tcagtgatgt agagtgcggt agccaatcgt tcgagatccg
    16381 cgactccctt ccggcccagg ctcttcgcca cgaaatcgat gcggccgcca tactccgcca
    16441 gggtcttaga atagagctcc tcgatatatt tgctgcgctc cgtggctgca atccgcgggc
    16501 cataaggccc ctgcggttca agcctcacca aatcatccgc acgcaatgcg gtgagttcgt
    16561 ctctcaggtc aaacgagaag ggcccatgct tgtacagaat gaaatcgaac cccaaaggaa
    16621 cttgcatgag acgctgtaag aagaacgtgg ccttctggac atgggtttcg ccacaccagc
    16681 tttcctcctc gcgcagcctc ttaattaagc gcgtaagcaa cgcggctttc tttagcctat
    16741 ccattgtctt cctccttctt cggtcgtatg atcttgtctc gatgttgtct cagatacttt
    16801 tccgcttcgt tgaataccgc tcgttcggcg aacacgtagt cgacggaaac gacgggtatc
    16861 gtgttcagcg cttgcgagat ggccaacgat gaaacaattc gcccgtcgcg catctttacc
    16921 gggaaatcag aactgccgct tttctggtga tatcggtcgt gccggaaatt ctcgcttcca
    16981 aatttgtcgc ataaagcttc gaagatggct tttcctgcct ccggatttat ttcgacatcg
    17041 ctggggtttc gctgatagat aagcttgaaa tgatcgcgtg caacgatccg acgcgcatgt
    17101 acgtgtcccc gtttcttttg gtcatgcgcg gcatccaata gcgcagcggt gacttcgttg
    17161 tctgtcattc ttaagtgttg ctgtagatcg gtggggaatc gggcatcagg aagccattcc
    17221 ctaaggaagt cggtcaaatg gatgtcatag attctacgaa ctggatgaca gtagacctgg
    17281 gaatacatga aataacgggc gagcaataag gcctcagcgg aatgaattcc gccttcttca
    17341 accccaagag ccaattcctc tgagctttca ttgtcgtctc cgcatgatgg catgggaaga
    17401 atacggaggg tgtcaatcag ccgataatgg tcgaacttgc cgtatgcgac gccaatatgg
    17461 tgggaatccc gcaagagata gtccatgcga tcgaccccaa acgcatcacc gacgatgatt
    17521 tccgaaagaa tggtctccca atcattgaac gtaaggtgtt ttgctttctt gggtcccacc
    17581 gcaagtttca ccacatcttc aactcgaaga ggtggcgtaa tcctattcca aatctcctgc
    17641 atctcttcgc tagcgataag ctcccgcgta agtctttcgt gatcccattc ttcggggagc
    17701 agctcttttt ccgcagcatg ggaaaacggc aagtggccaa tgtcgtgaca tagggcggcc
    17761 atacgaagcg tacggcgcca gtatcgcaac ttatcagaat cagtgagcgg ccgaagacgg
    17821 tctttcattt tttctgtaac gttgtctggg tgcgtgacaa catcatagac gcggcttgcg
    17881 agctccataa cgccgagaga gtgttcaaac cgcttgtgcg tggcggccgg atagataagg
    17941 tacgtcaacg ccagttggtg aacgtggcgc agccgctgaa aggggcgcga gttcacgacc
    18001 tcgcgttcgt cgttgtccat ctggacaaat gcgtggatcg gatctctgat ttcgtgaaaa
    18061 ttcctcgaca ctgtcaatcc ttcctttcgg ctgcccgtac aaccgcctcc gctggaatct
    18121 caaagaaccg tgctgccacg atctcacgct tcgcgtggtc cagccgcacc gcagggttct
    18181 ggattcggac aagctccggc ttcgggccga gcgggtccca cacgacgtac aaccagtagg
    18241 tctcccctag ctgctgggcc atgtaccact cgttggtcgt caggcggacg gcctgcccgc
    18301 gcctccggcc cttgacctca atgcgcttca cgacgattgc gcccgtcgta gggtcagcga
    18361 tccggtgcgc gcaacaccct ggagatctgc cccttgcgca tggccttctc ccgcgaggcc
    18421 tctctgctaa tggcctcgat gggcaggtag tcttcaatga gacggcggtc gtttacctta
    18481 ccgatctccg caaaccgggc gctcaagctg ccggagtctc ctctcgatct gcatgcgtcc
    18541 ggcctgctgg aggttcggcg tgtagtgggc cgcatagtaa agcgtgcccc tgcagttccg
    18601 tctccactga ctcgcgcgct catgattcag aacacgctcc tggacattgt gcgattcccc
    18661 aacgcccaag cacgtccact tctctccgtc cttgcaccag acgacgtaca cccctgcccg
    18721 cgcctgcagc tgattcgctg aggtgaaggc accctcgaag tggtagccat ggagttcgaa
    18781 gttcatgcgg attctcctgt cagcgtcggt ttctgtgctc gttcacgggg ttcttgctct
    18841 tgtcaagccc gctggccccc caccacaggg gaccaacaat cgtcatcttg aacaggggga
    18901 tttggcttgt caagcgcctc ccgccgccca agaatttgcc agaattcttg ggtttgccgg
    18961 gcgggccatt ggcctcgatc gggcggccag ggatgcgtat tctcccgcct tatgaagctg
    19021 tgggaaccaa agtgcgattc cgaccatgac cgcctggtcg aatggatggc gaagctggag
    19081 gaagagctgt tcgcggtaat ggaccgtaac gcggagttga aagaggaaaa cgcgcggctg
    19141 cgcggagaag tcgctgacct gaagcagcag ttggccgccg cgcggaagaa ctcgtcgacc
    19201 tcctcgaagc cgccatcgag cgacatcgtc aagccggaga agccgttgcc caagggcggc
    19261 aagaagcgca gcaaggacgg acagccggga cacgaacagc acttgcggtc gccgttccca
    19321 cccaagggcg gtcaaccact tgcagcgcta cacgctcgac tgctgcccgg actgcggccg
    19381 caggctggtc gcttcgcgct gtgagccgga actcctctag caggcggaaa tcaccgaaac
    19441 gccgaccatc gtgagggaat accaagggct tgcctactgg tgcccgcact gccgcaaggt
    19501 gcactacgcc ccgctgccag agcgcatggt gtaagcgggc tgttcggccc gcgattgacg
    19561 gccctggtcg ccttcgcgaa ggccgtctgc caggcctcgt ttttgacgat ccgcacgttc
    19621 ctccccgacg tggtgcggat atacgtttcg cgcggctacc tggccaagct catcggcaag
    19681 gtgagcgagt cgttggcccc gtcgtatgcc gagttgttcc aacggttgcc gggcgaatcc
    19741 gtgctgaaca tcgacgagac cgggcacaag gagaatggtg cgagattctg gacctcgtgc
    19801 ttccgggccc agatgtacac gtttttccgc atcgacaagt gccgcggctc gaaagtgctg
    19861 ctggaggtgc tgggcgagga gttcaacggc gtgccgggtt gcgactactt cagcgcctac
    19921 cgcaagtaca tgcgggactg cgacgtgctg gtgcagttct gcatggccca tctgatccgc
    19981 gacgtgaagt tcctactgac gctccccgga cgcgaggtcc aggcttatgg cgagcctctg
    20041 cgggacgcct tgcgcgacct gtttggcgtg atccaccgcc gcgagaagat gtctgcggcg
    20101 ggttttcaga aagccttgga ggcggcgcgg gagcaggtga tcaaggcggc caccacccgc
    20161 gtgccggagg cgaagcacgc ccgaaacctg cccgcacggt tccgcgtcat cggggaagcg
    20221 tacttccgct ttatcacgac gctggccatt gaggacgacg aataacctgg cggagcaggc
    20281 gatccgtttt gtggtgatcg accggcacat cacctaggga acgcggatcg cgaccatcct
    20341 gcggatggta ccgggtcgcc gttggtgcga acgcatctgg acggtgatcg cgacctgtgc
    20401 ccaacagggt cgtgcggtct ttctgttctt gctcgattcg gtacaagctc atctttgcgg
    20461 tggtccccca ccctcgctct taccgtcagg ctcctaacgg ccctctcctg atctctcttt
    20521 ctccgagatc tccgtggtga ttccctgcca ccccgtgaac ggctaccggt ttcttataca
    20581 tcgagaacag cctcctcgct caccggaaga tcagaactcg gcttcacctt cagccaaccc
    20641 agcatgctca agcctcgaat ggtcaccgcc acatcttcct ccttcagagc cgggcgtctc
    20701 ttttgcttcc aactcatcac ctcgctcagc acttctcgtt cggtcggctc tgatttcttc
    20761 ttcaaggcaa ggcccttggc ggcgaaatga accgtcgcag ccagctctgc ctgctccgtg
    20821 cgcatccgca tgaacaggtc ggcgagcgcc tcgatggtat ccttccagtc cgcaagatcg
    20881 cgttcataag cgcgccaggc atccttgaac gtcggccccg gcttaacagc gaacatgcga
    20941 cccaggcgtt cttcacgaat cagcccgttg ttcaccagtc gagtgatcag ccccttcagt
    21001 tcgggcgcat aaggcccgta gctgcttcgt cggtatgaaa gcccagtagg aattccagac
    21061 tccgtggcga agtaggcgat tttttggaac gaggtccgtc caacgggata gtggtagcgt
    21121 tcgttctcga tctcctccag gatcttcacc agcgcgaccc atgccggttt catccggctt
    21181 tccggagtgc cgccaggcgc tgcctccagc ggcgcctgct cgagaaaggt gggctgcagc
    21241 tcc
//
LOCUS       DAOSUT010000067        21178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1626_length_21178_cov_9.718506, whole genome shotgun sequence.
ACCESSION   DAOSUT010000067 DAOSUT010000000
VERSION     DAOSUT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21178
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_21178_cov_9.718506"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLD05_07855"
     CDS             complement(<1..252)
                     /locus_tag="PLD05_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77391.1"
                     /translation="MFRFVSHLITRYWPLVFVGWGAVFVILHLAAPHWDDVARDGDFA
                     YLPARAASVQAEKLRAAAFGEANAQSDVVIVMARRDGPLT"
     gene            complement(603..1019)
                     /locus_tag="PLD05_07860"
     CDS             complement(603..1019)
                     /locus_tag="PLD05_07860"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOP77392.1"
                     /translation="MTLVWDTTMSTGIPRIDAQHQELIRKLNGLLHAMQKGRGKEEIE
                     KLLDFLAEYVIKHFSDEEAEMERVNCPAAIANRIAHQHFIKRFTEFRERFKKEGASPR
                     IVIDVQKTLMDWVIMHIRGVDTKLAASVTRKAEART"
     gene            complement(1339..1755)
                     /locus_tag="PLD05_07865"
     CDS             complement(1339..1755)
                     /locus_tag="PLD05_07865"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOP77393.1"
                     /translation="MAVAWDQSLSTGVAKIDRQHQELIANINSLLEAMRSGRGRDEIG
                     KLIAFLSDYVVKHFADEEAEMDRVKCPVAVANRIAHQKFMTRFKEIQERFNKDGATPK
                     LVIDVQQAVLDWFIAHIRSIDTKLASSAKVKVGVGS"
     gene            2052..3614
                     /locus_tag="PLD05_07870"
     CDS             2052..3614
                     /locus_tag="PLD05_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77394.1"
                     /translation="MVRRFRLRFHKVWLSLLLIAGGCAATAGHLGGPFSPGAPQVTQS
                     SPGASESPNPNSLTTATPAAPGSASPDISKPASPGSAQSASSAGSAGPGSSSRESAAG
                     NNIVSVDLLVSELEAEGLLDAESKAQLTADLCRTPPHLWEGIVAAFRASMQLRKQKSD
                     QGQLQSSDVFIEDKDVRPAAGHTSTGSLPPQDSRASSQTGLTSVVQGAIPDDRARLPR
                     LASMDRFSDIRKEQASSQMDTQVLSEPEARPDSPIASQIARSLPARPSPPGGQETKSD
                     AFKSPSPSSEEAAGTWDYWVTRAVRDLEDQQTRSTREEIQLRLLRLALIRREGALEPI
                     PGLSPSLQDFWGQTLFGLLLLEDTQLHSDQQLRLVEARRHLENGIRRLAEECPLEVTG
                     LAFVTSVQSWGVYDAFEQYEFLPGQKVLLYAEIENLASESTAKGYRTAWRSSYQILDA
                     TGKQIASYEYAPNEEYCRRPRRDFFIGCELSFPTDAPPGRYTLRLTVVDLIKQRVGQG
                     TVDFTLRSRQAK"
     gene            complement(3760..4140)
                     /locus_tag="PLD05_07875"
     CDS             complement(3760..4140)
                     /locus_tag="PLD05_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77395.1"
                     /translation="MSKRVLIADDSVLIRRMIAEVLTQAGYEVVGEATNGAEAVAKYR
                     ELNPDVVTLDIVMPGTDGLYALDGILALNPNAKVVVVSALNQTKLISDAIRKGAQDFI
                     AKPFLPEQLLDTVSSCLCESSVPA"
     gene            4613..4906
                     /locus_tag="PLD05_07880"
     CDS             4613..4906
                     /locus_tag="PLD05_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77396.1"
                     /translation="MELNRYHYYMLGLFLLLLGLELRTIEAVVLTPEVTKFLAERTGH
                     PVATASNAVAAVTGTSPPIPPKTIRPPEWLGYAVLSAGSVLVLYSLALPKPGG"
     gene            4997..5464
                     /locus_tag="PLD05_07885"
     CDS             4997..5464
                     /locus_tag="PLD05_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77397.1"
                     /translation="MFSTTNVCRRKLGQVLASVTLAGIVRSLFAMEQASLPSENATAN
                     APDSGREGKSAEKSAPTRPRLREGTKLFDLVGTVRQTGRRYTFFVAANSNRFVLLENL
                     MLERILRAQASHGVTIQWKISGTVTEFQGQNYLLIEHALLHASSQEEPKPNVP"
     gene            5674..7017
                     /locus_tag="PLD05_07890"
     CDS             5674..7017
                     /locus_tag="PLD05_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77398.1"
                     /translation="MQHSEQRRTVNRRSFLKASAIGALGATAAFRFAHGALNEDFQVK
                     IGLIGCGGRGTGAVLNALGAGTRVVYPAKGYHTEDVAEDASVTRKDVQVTALADLFPD
                     RLESCRANLAKLGINVPPERCFVGFDAYKQLLALDDVTYVILATPPHFRPEHLMAAVN
                     AGKHVFMEKPVAVDVPGVKTVLQAGQIAKQKNLGIAAGTQRRHERRYQETIKRIQDGA
                     IGEIIYAKCYWNGGEIWVVEREPHWSDMEWQLRNWNYFCWLSGDHIVEQHVHNLDVMN
                     WVLGAHPVRAVSGLGGRQVRTDPKYGNIYDHFAVEFEYPNGVTVFSQCRQINGCLNIV
                     GEFVLGTKGHSDCQQTIQTGSERWRYRGPNPNPYEQEHEDLIASIRAGQPINEAEAVA
                     YSTMTGILGREAVYSGQAITWDDAMQSTTRLGPERYEFGPYPVQPVPLPGQYRFS"
     gene            7451..8038
                     /locus_tag="PLD05_07895"
     CDS             7451..8038
                     /locus_tag="PLD05_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOP77399.1"
                     /translation="MGMDDADHTRATVSASVDWAEALRQRERWLRAVIFARVREAQAV
                     DDVFQEVALAAVEQKAPIQDPAKVSQWLYRLAVLTSLMFRRRQGRRRKWIRRYAEDLR
                     PHTRDGYDPLAWLISSERREAVRQALDRLPAKDVEILLLKYGQDWRYEEIAEHLGIST
                     SAVQTRLFRARQRLRRELDQMAHADSIGNESGHHT"
     gene            8048..8941
                     /locus_tag="PLD05_07900"
     CDS             8048..8941
                     /locus_tag="PLD05_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77400.1"
                     /translation="MTIPSREEQASAPELEGFIMDQFDNEDISQEIIDRLVDGELDET
                     VRAAVLRRLEADPLLCKRIAIAFLENQAWREAFSTYCSALAPRTKSEGSLGTQFFEKR
                     RREAQSNEIGPGEALSKDTLPQIIKPSADMPRRCPQVSTLGWLVAVAASFILAFLASW
                     YIQSFWLTGARYKPTLSAGSLAANQGQVSSENQAVPVKPDGPMAVSPHIEMVSVPLPV
                     DDAGTWATMEVPFISFPGAEVTEVLPGGMIPTEILESLGAQGHVVEQERRFVPVALPD
                     GRQSVVPIDQVRIRFVDRPKR"
     gene            8969..9979
                     /locus_tag="PLD05_07905"
     CDS             8969..9979
                     /locus_tag="PLD05_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOP77401.1"
                     /translation="MNVSLTLRRILWKTLLTAIFVSIICMNIPGVAVFGGTLAENPAA
                     AEKATKKDTKPDDGGKSEKQEKTEYWLGISCGPIPDFLRAHVAIPENEGILVHAVAKN
                     SPAERAGICQHDILLAAANKVLKSPEDLVAVVNEKKDQSFEIKLLRKGKTETVTVTAE
                     PRPAEAKEEAPPFPGPWASTEAWEKWFEWFRKQLPNQPPLTFRFYRPGIVVPPGEAED
                     WPKDLTIVITKEGSQPAKIVVRRGDKTWEVTEKELDKLPKDIRPHVERMLRGSRGIPG
                     GLVEVKPFGQLTPPKELEKDLVRPFRGWLSPDTERLERIEKRLEELNRRLDELQKKDK
                     SE"
     gene            10292..11161
                     /locus_tag="PLD05_07910"
     CDS             10292..11161
                     /locus_tag="PLD05_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77402.1"
                     /translation="MNLIGKIFVVLIFVMSLVFMSFAIMVYSAHINWREVVENPKATP
                     DKPLGLRLQVQNLRKDNDALKAQLDELTKKLDAEIADKRAQVAKLEQERDDLQAERDQ
                     LMAQVAQLTQQTRDAVAAMEAAHQTLAKRTDEVGNLRDLLRQAEQARHDALVRLVQRT
                     DELHQVANELRALKERAVTLAADLAKAREVLTKFDLKAEPELYRDQAPPVEGMVMAAP
                     INGLVEISIGADDGLMKGHKLEVVRLGNGENKYLGRIEVVRTEPDRAVCKIVPEYQRG
                     PIQEGDRVVSKIW"
     gene            11168..11374
                     /locus_tag="PLD05_07915"
     CDS             11168..11374
                     /locus_tag="PLD05_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77403.1"
                     /translation="MQYRSGQATIYTVLLAIALVAIIVGCVFLFLEVQDYGSPPYPEG
                     GVVQALTVPQQLAWQSPEPGWSLG"
     gene            complement(11694..11927)
                     /gene="csrA"
                     /locus_tag="PLD05_07920"
     CDS             complement(11694..11927)
                     /gene="csrA"
                     /locus_tag="PLD05_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOP77404.1"
                     /translation="MLVLSRKKNESIVINDQITIVVVEIRGDKVRLGIEAPKDVPVHR
                     REVYEAIKSAQKALSQGTDQNASASSASTSETT"
     gene            12390..12947
                     /locus_tag="PLD05_07925"
     CDS             12390..12947
                     /locus_tag="PLD05_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOP77405.1"
                     /translation="MGLSYVAFGIFRWWQNIALSVVTTIRALKVTLGTWVSTYHPARR
                     TFTHQYEYPERPAQVTPRYRGFHRFDLTSCIGCERCARDCPVSCIYITKERVPGRKGF
                     RVTSFVIDYTKCMFCAYCTENCPVDCIFMGSTYDLSCYSRDGCLVDFAKLPLEIAWGR
                     ASLNPTAVALSKSVLEPVDEGPGGA"
     gene            13260..13847
                     /locus_tag="PLD05_07930"
     CDS             13260..13847
                     /locus_tag="PLD05_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOP77406.1"
                     /translation="MSGVALVGYFTVFGALAVGLVFGALLLGKLFRPDNPTPIKKETY
                     ECGEPSVGSADIQFDIRFYVMALVFLIFDVEVALFFPWATVFGKATHVASISVDSASR
                     RERLAELGITEATLPPLSGPKRPATLSNGISVGQQAAGPTGLPPVEELSAWGRQLATL
                     ALCDILVFFGVLMVGFAYVWCRGDLTWVRAVPHRA"
     gene            13943..14473
                     /locus_tag="PLD05_07935"
     CDS             13943..14473
                     /locus_tag="PLD05_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOP77407.1"
                     /translation="MSIEEIFQHLQEKFGEAVRPGDFTSIDPWIEVGPEAIADVCRFL
                     RDNPALRFEMLHCITAVDYLEVDEKKAAKASFVPHLELIYHLSSLTLRHRVTLKVMLP
                     RWRDDHPGQLPRVPTVSNVWKTALWHEREVYDLSGVEFVGHPDLRRILCPEDWVGHPL
                     RKDYQMPIEYHGIRAR"
     gene            14599..15813
                     /locus_tag="PLD05_07940"
     CDS             14599..15813
                     /locus_tag="PLD05_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOP77408.1"
                     /translation="MNVIPGVDPEDFSVTSETEVRTEELLINMGPQHPSTHGVLRVVL
                     RTDGELVLEAIPHIGYLHRCAEKIGEKVTPRQFIPYTDRMDYLAGMNMNLGWALTVEK
                     LLGLEVPEKARHLRVLIVELNRIASHLVAAGTYGLDLGSFTPFVYCFREREKILNLFE
                     AVCGARLTYSYITVGGLMADLPSGWLDQCEAFLDQFEPVIDDLHDLLSVNHIFVKRTA
                     GVGVLSRELAVDYGCTGPVLRGSGVDWDLRRDGDPFYTRMYRDYDFEVAVCRDGKYPQ
                     PSPPVPTEAVLGDCWHRFFVRMVEVVQSVRLVRQAIEKYRLAEGDIGQPIKLNQKLPP
                     GEVYLETECPKGQMGFMIVSLGEAIPWRVRARSSSFCNLSVLPELCRGCLVADVPAIV
                     GSLDIVLGEVDR"
     gene            15881..17056
                     /gene="nuoH"
                     /locus_tag="PLD05_07945"
     CDS             15881..17056
                     /gene="nuoH"
                     /locus_tag="PLD05_07945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOP77409.1"
                     /translation="MAQWLSDWLTLSPQWEWVAYLTVALLQAVILLALVMTAVALATW
                     AERKVAGRIQDRLGPTRVGGRFGWLQPLADGLKLFTKEDLIPANADAVLFRLAPYLSF
                     APVFGVFMALPFSDGWVAHHFNTAAFFILAVLGLEVFGIILGGYASGSKWSLLGGMRE
                     AAQVVSYEVPLAFSAMLPILLAGTMDLVAIGEAQRGWFWNWFVFHDPFTFVAFWIYVI
                     CAVAGTNRAPFDLAEAESELVAGFHTEYSGFRWSVFYMGEYAAMAAVCGLGVVLFLGG
                     WNGPIPVAEWLFGPASSTSPIVNFLSQLLGAVNFIIKCIIGVVFMISLRWTLPRPRID
                     QVMSMCWKYCFPLAALAFLGATLWVLMLPDGLLGQGHPKASTCEQSVMVTVERIETP"
     gene            17053..17691
                     /locus_tag="PLD05_07950"
     CDS             17053..17691
                     /locus_tag="PLD05_07950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOP77410.1"
                     /translation="MTGPDGMAILFWLFAIVTCGGALVVVLSRHIVRAAVGLILSLFG
                     VAMLFLLAGAEFVGAMQLMIYVGGTVVLLAFGVMLTARRPLLPLSTTRDQWLAAVLLA
                     CPLTVILVQAALSYAPTDSVSAQVDAHSGVSTPVGMLPVTPARLGMALLGFSQDNHRP
                     DGGEVSHGDSGYLLPFELISVHLLVVLIGAAYLARREVPSGMTPRDNQGQLQ"
     gene            17722..18057
                     /gene="nuoK"
                     /locus_tag="PLD05_07955"
     CDS             17722..18057
                     /gene="nuoK"
                     /locus_tag="PLD05_07955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOP77411.1"
                     /translation="MFSPITPAHYLFVGIVLFVTGMICLLTKRNIIAILIGVELILNA
                     ANINFIAFSSPVLFPKRVPPLVLDGQIFVLFVMILAAAEAAVALAIALHFYSRFGTVD
                     VDQGDELKG"
     gene            18057..20372
                     /gene="nuoL"
                     /locus_tag="PLD05_07960"
     CDS             18057..20372
                     /gene="nuoL"
                     /locus_tag="PLD05_07960"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOP77412.1"
                     /translation="MSSLSLVPQLLLIAWMLPLASFVFIFLTGSFLDRRGRASGFIAT
                     AAIAGSFLLSVVAAALWFGYYPLDSNHQASSHPIRPLSGDWYTLAEFGSIKLALGYYI
                     DALTLAMYVMVSFIATLIHIYSFGYMHEELHEVNDPQVILGDGTTLRRPGRFARFFQY
                     LSLFGFSMLGLVIANNLLMTFVFWELVGICSYFLIGFYVERSSATSAGNKAFIVNRVG
                     DFGMVLGLAALLTSCGTLHFDHYQTTNGAHYAGLFSYAKAIQHPASELEPAEGSRSED
                     SQSNPETPPVTLSGPKGLTYGLLVLAGLALLCGCLGKSAQFPLHVWLPDAMEGPTPVS
                     ALIHAATMVAAGVYLVGRIYPLLTPEVLIVVAVIGTITLFMAATIALTATDIKKVLAY
                     STISQLGYMMLGLGTGGWVAGLFHLFTHAFFKALLFLGSGSVIHACGTNEMPQMGGLL
                     RKMPWTAITMLIGCLAIAGAGIPLWAGFSGYHSKDAILAQVYLFHTTNRFLGWIYYLA
                     LFSAALTAFYMFRLWFLTFIGQPRNEHIFGHAHESPKSMTVPLVVLAFFAVVAGWSVP
                     GTHWSLSALLEQARVTPLLENSQVPLFGNVVIPHEHESHEAQIHQLVSLEAFTAGILG
                     FAIAVVFYGARWLDPAKVCQRFFGLYRFLLNKWYFDELYSRLFVRPVTWVASVAAWID
                     RAVIDWTVDHAAVAVRSLAHLDDWLDRILVDGAVNRLAAAIYGMGEWFRQWQTGRIRN
                     YILWIVVGLVSLFILGSIYFGLGMHLVVGAS"
     gene            20433..>21178
                     /locus_tag="PLD05_07965"
     CDS             20433..>21178
                     /locus_tag="PLD05_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOP77413.1"
                     /translation="MVAGLVFLSIVVFLPAFGALVLMFFPSHAERAIRRTTLGVTLVV
                     FLLTLRLVLPGLHAASQNPLEQVLHDVRFAAGEPGYQHAYAVPWISSFHIDYFLGVDG
                     ISLPLVLLTSFLSVLAAAASWSVTKHVKAYSILFLLLETGMLGVFLALDFFLFYVFWE
                     VMLLPMYFLIGVWGGPRREYAAIKFFLFTLVGSVLMLIAILLLYFASDVRQLPENARR
                     ESFLSQEVMVKSLAPSEKPLHTFNLIALAKL"
BASE COUNT         4622 a         5574 c         5839 g         5143 t
ORIGIN      
        1 agtaagaggg ccatctcgac gggccatgac gataaccaca tcactttgag cattcgcctc
       61 accaaaggcc gcggcccgta gtttctcggc ctgaacactc gcagccctgg ccggcagata
      121 ggcgaaatcc ccgtctcggg ccacatcgtc ccaatgggga gccgccaaat gcaaaatcac
      181 gaacacggca ccccacccga caaaaacaag cggccaatac cgtgtgataa gatggctcac
      241 gaagcgaaac actgcgttcc cccaaatgat tccaacgcgc gtcaagcctc cgcaatggcg
      301 gatgctccat cacaagaccg atcgccgagc cgccgttact ttaatcctac caaatcttcc
      361 cattcatcca agatgaatgt gcggttccat ccacctgcta acgttagcca gattaacaaa
      421 aataccgctt acagaagagc ggtaaacagc gcaaaatctg acagtcccgg cgacgttgcg
      481 gctgccgaga ggaggaggac gtgaacgtcc tcgcaatcag caaacaccgg gggctatcct
      541 gctgacgagt gcgtactcga ttggctgttt cggctcgtcg aacgataacg gtatcctgat
      601 gttcaggtcc gagcttcggc ttttcgtgtt acgctggcag cgagcttcgt atccactccc
      661 cgaatgtgca tgatcaccca gtccatcaag gtcttttgaa cgtcgatcac gatccgggga
      721 ctagctcctt cttttttgaa ccgttctcta aactcggtaa atcgtttgat gaaatgttga
      781 tgcgcaatgc gattggcgat ggctgccgga caattgaccc gttccatctc tgcttcttcg
      841 tcggagaagt gcttaatcac gtactcggcc agaaagtcga ggagtttctc gatttcttcc
      901 ttccctcggc ctttttgcat agcgtgcaat agcccgttta gcttgcggat caactcttgg
      961 tgctgagcat cgattctcgg aatgcccgtt gacattgtcg tgtcccaaac aagcgtcata
     1021 gcaaaaactc ctcaatagtc tggaaaacga tctttctctt tccctaaaac ctgcgagtga
     1081 accgacccac cgaagcttgt cggactcttg cactcacaag caaaacactc tcgcactgaa
     1141 ccacccctgg ctccttgttc taaagccagg tttgagttgg tgataactcg tttaaaatgc
     1201 gtgccgggcc aggattcgtg gtgcaacata caccgaagcg tcgttcttgg tgcgaaatcg
     1261 cacacttacg ccgcagcgga tcgaatttgg gaatttctgg ttcctttcgg aaattggcaa
     1321 acgaatccga gttatggatc aagaaccaac tcccacctta accttcgcgc tactggcaag
     1381 cttcgtgtca atgctccgaa tgtgggcaat gaaccagtca agcactgctt gctggacgtc
     1441 tatgaccaat ttgggcgtgg caccatcctt gttgaagcgt tcctggatct ctttaaatcg
     1501 tgtcatgaac ttttgatgtg cgatccggtt ggccacggcc acggggcatt tgacgcggtc
     1561 catctccgcc tcttcatcgg cgaagtgctt gacgacgtag tcacttaaga acgcgatcaa
     1621 ctttccgatc tcatctctgc cgcggccact ccgcattgct tcgagaagcg aattgatgtt
     1681 cgcaataagc tcctggtgct ggcgatcgat ttttgccacg ccggtagata aactttggtc
     1741 ccaagcgacg gccatagcgg gtccccctat gttcttagtt ttttgccaat atcaccaaac
     1801 cacacaaacc tctcacgtac accatctacc ctaacttagc tcgcaaattg ccctcgtgag
     1861 gcgaaacacg gcgcggaacc atcctaatga gtggcaatgc accccactgc cttgtgcata
     1921 ggccgaaacc caatcttctc ggcgaacttg cccctttttc tgtcgtttgg taacatccac
     1981 cgccccaaaa ccacaaaacc ggaggaaaac ccgtctctcc tcgggcacgg cggttggaac
     2041 caagaggttt tatggtcagg cgttttcgct tgcgatttca caaagtctgg ttgagtctgc
     2101 tcctgatagc aggggggtgt gccgccactg cgggacacct cggtggtcct ttttcgccag
     2161 gagcgcctca agttacgcag tcttcaccgg gcgcatcaga aagtcctaac cccaattcac
     2221 tgacaactgc caccccagcg gccccgggtt cggcctcacc agacatttcc aaaccggcat
     2281 cgccaggttc agcccaatca gcatcctctg cgggttcagc aggtcccggc tcaagttcgc
     2341 gggaatcagc cgctggtaac aacattgttt cggttgatct gctggtatct gagcttgagg
     2401 ctgaaggtct gctcgatgcc gaatcgaagg cccaacttac ggcggacctc tgtcgtactc
     2461 cgccacacct ttgggaagga attgtggctg catttcgcgc cagtatgcag ttgaggaaac
     2521 aaaaaagtga ccagggccag ctccagtcga gcgatgtatt catcgaggac aaagacgtcc
     2581 gcccagccgc cggccacacc tcgaccggat cattgccgcc ccaagactcc cgagcaagct
     2641 cgcaaacagg gttaacatct gtcgtccagg gggccatccc tgatgatcgc gcgaggcttc
     2701 ctaggcttgc atctatggat cgtttctcgg acattcgcaa agagcaagcc tcatcgcaga
     2761 tggacaccca agtcttgtcc gaacccgaag ctaggcctga ttcgccgata gcttcccaaa
     2821 ttgcccgtag cttgccggct cgcccatcgc cgccaggtgg tcaagaaacg aagtctgatg
     2881 catttaaatc gccgtctcct tctagtgaag aagcggccgg aacgtgggac tactgggtaa
     2941 ccagagcagt tcgggacctc gaggatcaac aaacacgcag cacaagggaa gaaatacagc
     3001 ttcgtctctt gcgcttggcg ttaatccgtc gtgagggtgc tctagaaccc ataccgggct
     3061 tgagcccatc tttgcaggat ttctggggtc aaacactttt tggactcctg ttacttgaag
     3121 atactcagtt gcattcggac caacagctcc ggttggtaga ggcacgccgt cacttagaaa
     3181 acggcattcg acgcttggca gaggaatgcc cgctcgaggt gaccgggttg gcgtttgtca
     3241 cttccgtcca gagctggggg gtgtacgacg ccttcgaaca gtatgagttt ttgcccggtc
     3301 aaaaggtcct cttgtacgcc gagatcgaga atcttgccag cgaatccacg gcgaagggct
     3361 accgaacggc ttggcgaagt agttaccaaa tcctcgatgc cacaggcaag cagatcgcta
     3421 gttatgagta tgctcccaac gaagagtact gccggcgacc acgtcgggat tttttcattg
     3481 gatgtgaatt gtcctttccc accgacgcac cgcctggccg gtacacgctg cggctcaccg
     3541 ttgtcgattt gatcaagcaa cgagtagggc aggggacggt ggactttact ttgcgatctc
     3601 gccaggcgaa atgacggcct acgcttcctg ccgtaagaat gatcgctgat caccaacttg
     3661 accgtcagtg agctgtccgc tttaacgcgt cggaaagcaa tcatgcgtca cgaaacgggg
     3721 aaccgtttcc ttcggtgatt tattttctag ataaaaggct catgccggaa cggacgattc
     3781 gcaaagacaa ctgctcacag tatccagcaa ttgctcgggc aagaacggtt tcgcaatgaa
     3841 gtcctgcgca cctttccgga tggcatccga gatgagtttc gtttgattga gcgcactgac
     3901 aaccactacc ttagcgttgg gattgagggc caaaataccg tctaacgcat aaagaccgtc
     3961 cgtgcccggc atgacaatgt ccaacgtgac gacatcggga ttcaactcgc ggtactttgc
     4021 cacggcttcg gcaccgtttg tggcttcacc gaccacctcg tagccggcct gggtgaggac
     4081 ctcggcaatc atccgtcgaa tcagcactga gtcgtcggca attaaaaccc tcttggacat
     4141 gattgactcc taggcagttc tgtgcacgct cccagatgat cctaccgtcg cacattaagt
     4201 taacgtggcc gatccaatct gcttcgtagc ttggtttctg gaacacaaca gccgacctat
     4261 gcgaaccgtt cactaaaaca gtaggtcaca aatcggcgat ctgccacaaa tccgcgcatt
     4321 gtagagttgg ccgcttgcaa tgctttgcat tgtcttttga attatcgcac gcccgcatcg
     4381 agccacagcg tattaggcag cgtattccca ttgcaatccg caaaaacggc atcggcccac
     4441 ttatgcggca tatccgccac gtgcgaagcc tccatcataa tccgattggt aggtcccgta
     4501 tctaaccaca gtttgtgaaa tccatcacgc agcttctcac ctaacgcaat ccgcacagcc
     4561 gatgacaaaa aagggaactg cccctggtgt caagacaaag gcattttcaa ccatggaatt
     4621 gaaccgctat cactactaca tgttggggtt gtttcttctt ttgttggggc tggagctccg
     4681 gaccatcgag gccgtagtcc taacccccga ggtaacgaag ttcctggcag aacgaactgg
     4741 tcaccccgtg gccaccgcca gcaatgccgt tgcagcggtc acaggcacgt ccccaccaat
     4801 tccgcccaag accatccgcc caccggaatg gctcggttat gcggtcctct cagccggatc
     4861 ggtcttggtc ttgtatagcc ttgccttgcc caagccaggc ggttagcaag aaaagaacac
     4921 cggctagtcc tggcccagct tcatttccgt cagagcgggg gcactcgtaa cctatccacg
     4981 gggagaggga agctcgatgt tttcgaccac caacgtttgc aggcggaaac ttggccaagt
     5041 cttggccagc gtcacgctcg ccggaatagt acggtcgctc tttgcaatgg aacaggcgtc
     5101 attaccatcc gagaacgcca ctgctaatgc acccgattcc ggccgagaag gtaaatcggc
     5161 agaaaaatcc gcgccgactc gcccccggtt gcgagaagga acaaaactct ttgacctcgt
     5221 tggcaccgtt aggcaaaccg gtcggcgtta cacattcttt gttgctgcca atagtaatcg
     5281 ctttgtcctg ttggaaaacc ttatgctgga aaggattctc cgagcgcagg ccagtcacgg
     5341 cgtcacaatc caatggaaga tttccggcac tgtgaccgag tttcagggac aaaactacct
     5401 gctcatcgag catgcccttt tgcatgcgag ttcccaagaa gagcccaagc cgaacgttcc
     5461 gtgaggggaa aacctcatgc aacctcacgg gaggtttcct tacatctttt cgaaactacg
     5521 tctcgcctcg ggggcgtctg tctggcacgg tcgctaaaac atttcgaaac gccccggtcc
     5581 aataaaccag ttgcttaccg ccaacgtcat gcgtatcttt gattcttgct cgatgtaaca
     5641 accagaagga acagttcttt caggagatcg gtcatgcaac actcagagca gcggcgaacg
     5701 gttaataggc gatccttctt gaaagcgtcc gcgattgggg ccttgggagc cacagcggca
     5761 tttcggtttg cccatggtgc tttgaatgag gattttcaag taaaaattgg cctgattggc
     5821 tgtggtggac ggggaacagg tgcggtcctc aacgcccttg gggccggaac aagggttgtt
     5881 tatcccgcaa agggttacca tacggaggac gtggctgagg atgcctccgt cacccggaag
     5941 gacgttcagg tgaccgcatt ggctgacttg tttccggatc gtctcgaatc atgtcgggcc
     6001 aatctagcaa aattgggaat caatgtcccg ccggaacggt gttttgtggg ctttgatgct
     6061 tacaaacaac tccttgccct agacgatgtg acatatgtca ttttggctac tccaccacat
     6121 tttcgcccgg agcacctgat ggccgcggtg aacgcgggaa agcatgtttt tatggagaaa
     6181 cccgttgccg tcgatgtccc tggcgtcaag actgtcctcc aagccggcca aatcgctaag
     6241 cagaagaacc ttggcattgc tgcaggaacg caaagacggc atgagcggag gtaccaagaa
     6301 acgattaaac ggattcaaga tggggcgatt ggcgagatca tttatgcgaa gtgctattgg
     6361 aatgggggtg aaatctgggt tgtggaaaga gaaccccatt ggtccgacat ggaatggcaa
     6421 ctccgcaatt ggaattattt ctgctggttg tcgggcgacc atattgtgga gcagcacgtt
     6481 cacaacctgg acgtcatgaa ttgggtcctc ggggcccatc cggtgcgagc cgtttcggga
     6541 ctcggcggac gacaggtgcg gaccgacccg aaatatggca atatctacga ccactttgcc
     6601 gtggaattcg agtaccccaa tggagtcacc gtcttcagtc agtgtcgcca gatcaacggt
     6661 tgccttaaca tcgttgggga attcgttctc ggcacaaaag gccacagtga ttgccagcaa
     6721 accattcaga ccggcagcga gcggtggcgt taccgcgggc cgaaccccaa tccatacgaa
     6781 caggagcacg aagacttgat cgcgagtatc cgggcaggcc aacccatcaa tgaagcggag
     6841 gccgtggcct atagcacgat gacgggaatt ctcggacgag aagcggttta cagcggccag
     6901 gcaatcacct gggacgacgc catgcagtcc acaacacgac ttggaccaga acggtatgaa
     6961 ttcggtccct atccggtcca gccggttccc ttgcctggcc aatatcgttt ttcttgagac
     7021 agggcgctct gaatcgcgcc gtaccacggc gactaaccga aaggcttcat taatgcgaca
     7081 ccggcaccct ttgtggtgac ggtgctcgtt tggtttgcgt gctctcataa gcgcgtcacc
     7141 aaagatagtc gagacgactc ctggacgacc tcggcgtaac gtccgatagg tcaattgcct
     7201 ggtcactacg atggacgccg ggggagcgtt ttcgcaatcc tgaaacgctt cagaagcccc
     7261 tttcagtgaa cacaagcgtg aaccaactct gggtagcgct agcaaggggt acggcatacc
     7321 acggctgtgg catagcgaac ctcaagaggg tcgccctgcc ggttaacata tctcgttgtt
     7381 gccggtactc aggtgccgaa acagcagggg caccagcggc aacattcttc tcggatcagc
     7441 agtaaagttc atgggaatgg atgacgcaga tcacactcgg gcaaccgttt cggctagcgt
     7501 tgactgggcg gaggcgctca ggcagcgcga gcggtggttg cgcgcggtga ttttcgcccg
     7561 ggtccgagag gctcaagccg ttgacgatgt tttccaagag gttgccctgg ccgcggtgga
     7621 acaaaaggca cctatccagg atccggcgaa ggtaagccag tggctttacc gcctcgccgt
     7681 tttgacttcc ttgatgttcc gacgtcggca agggcgacgg cggaaatgga tacgtcgcta
     7741 cgccgaggac ttgaggcctc acaccaggga cggttacgac ccgttggcgt ggctaatttc
     7801 ttcggagcgt cgggaagcgg tgcgacaagc gttggaccga cttccggcca aagatgtgga
     7861 aatcctgcta ctgaaatatg gtcaggattg gcgatacgaa gagattgccg agcatttggg
     7921 aatcagtacg tccgcagtgc aaacccgact gtttcgggct cggcagcgtc tccggcggga
     7981 gctggaccag atggcacacg cggattcaat tggtaacgag agtggccatc atacttaaga
     8041 cgttatcatg accattccga gtcgggagga acaagcttct gcacccgaat tggagggatt
     8101 tattatggat caattcgaca acgaggacat ttctcaagag atcatcgacc gccttgtgga
     8161 tggcgaattg gatgagaccg tccgtgccgc cgttttgcgc cggttggaag cagacccgct
     8221 tttgtgcaag cggatagcta ttgctttttt ggaaaatcag gcctggcgag aggcgttttc
     8281 aacttattgt tctgctttgg caccaagaac caagtccgaa ggttcccttg gaactcagtt
     8341 ctttgagaag cgacgcagag aggcccaatc caacgaaatt gggccaggtg aagcgctttc
     8401 gaaagatact ttgccacaaa taattaagcc atccgccgac atgcctcgcc gctgtcccca
     8461 agtctccacc ttgggatggc ttgtggctgt ggctgccagc ttcatactcg cattcttggc
     8521 atcatggtat atccagtcct tctggttgac cggggcaaga tacaaaccca cgttgagtgc
     8581 cggctcgttg gcggccaacc agggacaggt atcgagcgaa aatcaggctg ttcccgtcaa
     8641 gcccgatggg cccatggcag tttcacccca tatcgagatg gtgtcggtgc ctttgcccgt
     8701 cgacgatgcg ggtacctggg caacgatgga agtccccttc atttccttcc ccggggcaga
     8761 ggttactgaa gtgcttccag gcgggatgat tccaaccgag attctcgaat cattgggtgc
     8821 ccagggccat gttgtggaac aggaacggcg gttcgtcccc gttgccctac ctgacggccg
     8881 ccagagtgtc gtcccaattg accaagtgcg gattcgtttt gttgatcgac ccaagaggta
     8941 aggtttgcag attgaggagg tatcggccat gaacgtgtca ctcacgctaa ggagaatctt
     9001 gtggaaaacg ctactcacag cgatcttcgt gtcgatcatc tgcatgaaca tcccaggtgt
     9061 tgccgttttc ggtggcacgc ttgcggagaa ccccgctgcg gcggaaaagg ctacgaaaaa
     9121 ggatacaaag ccggacgatg ggggtaagtc tgaaaaacag gagaaaaccg aatactggct
     9181 agggatttct tgtggtccca tcccggactt tctccgtgcc catgtggcca ttccggaaaa
     9241 tgaggggatc cttgtccacg cggtcgcaaa gaacagtcca gcagagcggg caggaatttg
     9301 ccagcatgac atcttacttg cagccgccaa taaggtgctc aagtcgccag aggacctcgt
     9361 cgctgtggtc aatgaaaaga aagatcagtc attcgagatt aagcttctcc gaaagggtaa
     9421 gacagagacc gtcacagtga ctgccgaacc tcggcctgcc gaggcaaagg aggaagcccc
     9481 gccattcccc ggcccgtggg cttctaccga ggcatgggag aagtggttcg agtggttccg
     9541 aaagcaattg cctaaccaac caccgttgac atttcggttc tatcggccgg ggatcgtggt
     9601 tccccccggg gaagcagaag actggcccaa agacttgacg atcgtcataa ctaaggaggg
     9661 atctcaaccg gcaaaaatcg tcgtccgccg tggtgataag acctgggaag tgacggaaaa
     9721 agaattggat aaactcccca aagacatcag accgcacgtc gagcgaatgc tgcggggcag
     9781 ccgggggata ccgggcggac tggttgaggt caagcctttt gggcaactaa caccaccgaa
     9841 ggagttggag aaggatttgg tgcgtccctt ccgcggctgg ctctcaccgg atacggaacg
     9901 gctagagagg atcgagaaac gacttgagga gctcaatcgc cgacttgacg aactccaaaa
     9961 aaaggacaag tccgagtgat gatgaagctc cgagaaaaag taagctaacg tcgagaaagc
    10021 aaaacccaca tttaccccct cgtatgaggg ggttttcgtt gcgcatggtt ctaatgagct
    10081 ttgagccgct ccaacggagg ggcagggggg taaatcgttg tcgggattcg acggcaattg
    10141 tttgacatgg gtaagaccgg ctagttatac tcgaagcgta gaatgggata gctaaaacgg
    10201 cgtggcgtaa acgttctcgg agtagtgcga tttcgtgggc tccgagcaaa acggtgttag
    10261 gagctttttt cggtggtgga aggttgagcc tatgaatttg attggcaaaa tcttcgtggt
    10321 gctcatcttt gtgatgagtc tcgtgttcat gtccttcgcc attatggtgt actccgccca
    10381 catcaactgg cgcgaggtgg tagagaatcc caaggcgact ccggataagc ctctagggtt
    10441 acgcttgcag gttcagaacc tccgtaaaga caacgatgcg ttaaaagccc agctcgacga
    10501 gttgaccaag aagctagatg cggaaatcgc cgacaagcgg gcgcaagtgg cgaagctaga
    10561 gcaggaacga gatgatttac aagccgaacg tgatcaattg atggcccaag tggctcaact
    10621 cactcaacag acccgcgatg ctgtcgcggc gatggaggcg gcccatcaaa cgctggccaa
    10681 gcggacggac gaggtcggca acttgcgtga tttgcttcgg caagccgagc aagcgaggca
    10741 cgatgccctt gtccgactgg ttcaacggac cgacgagctt catcaagttg cgaacgagct
    10801 gagggccctc aaggagcggg cggtcacgct tgctgctgac ttggctaagg ccagagaggt
    10861 tctcaccaag ttcgatctta aagccgaacc cgagttatat cgtgaccagg ccccgcccgt
    10921 ggaaggtatg gtcatggctg ctccgatcaa cggacttgtt gagatttcca tcggggctga
    10981 cgatggtttg atgaagggcc ataaactcga ggtagtccgc cttggaaatg gcgaaaacaa
    11041 gtatctcggg cgaatcgagg tggtccgcac cgagcctgac cgggcagtat gtaaaattgt
    11101 gcccgaatat cagcgtggac ctattcagga aggtgatcgc gtcgtaagta aaatttggta
    11161 aaccccaatg caataccgat ctggacaagc aacaatttac acggttctgt tggcgattgc
    11221 cctggttgct atcattgtgg gctgcgtatt cctgttcctg gaggtccagg attacggtag
    11281 ccctccctat cccgaagggg gagtggtcca agcgttgaca gtgcctcagc aactcgcctg
    11341 gcagtcacca gaaccgggat ggtctttggg ctgagtatca cggcaacgcc atgatcgctt
    11401 ttcaacaaac tcccttacgg gagttttttg tttgaaagaa taagacaagc caggctcgcg
    11461 cgctggtgta atctctaaag aatgcagagg accgttgttc gaaaaaccac cacccaagag
    11521 gaacaccgcg tacgccaagc agggcttagc cccagtcggc tatccaaact cgcctcaacg
    11581 gcggaatcca ggggtccgaa cgaaataaaa caatctaggc aaattacgag agaatagttg
    11641 tcgccgcgaa tggacaaaca ggtgggctgt aacgtgaggc ggggttacat ccttcaggtg
    11701 gtctcgctcg tggacgccga agatgcagat gcgttttggt cggtgccttg agaaagggcc
    11761 ttctgggcgc ttttgatggc ctcatagact tcccgacggt gcaccggaac atccttcggc
    11821 gcctcgatcc cgaggcggac cttgtcaccg cggatttcaa cgaccacaat ggtgatctgg
    11881 tcgttgatga caatactctc gttcttcttg cgagatagga ccagcatatt tccattctcc
    11941 ttccatctgc tggggacggg ctacggtgcg cgcttgcgcg cactctcctc ttatttgact
    12001 gtaactccga acaccatgta aatcaagcaa aaaacgtgcc cggagtcgca tttccaggtg
    12061 cgtcaggtgt tacagcccgc cgttatggcc gagtcagcgt ttaaaaaata ttttcgctat
    12121 acagttgcgc ttctcgggcc caccaaattg acaggttacg gatagtccgg aacactgcga
    12181 gtgggcaggc tggcaacgtc ttgcaacaga aaatcggtcc cgaaggtatt cgctcacggt
    12241 ttctgaggat tttgtgtcgc ttggagctgc cattcgtccg tggcctttgg ctaaaatatg
    12301 tgattcctcc ccgggggcga gactttttta taacatgact atctggcata ataggaataa
    12361 atgagaacac agacgcggga gtgcgagcag tgggattaag ttatgtagcg ttcgggattt
    12421 tccgatggtg gcagaacatc gcgttaagcg tggtcaccac gatccgggct ttgaaggtaa
    12481 ccttaggcac ctgggtctca acgtatcatc cggcccggcg gacattcacg caccagtacg
    12541 aatatccgga aagaccggca caggttaccc cacgatatcg ggggttccac cgttttgacc
    12601 tgacttcctg catcggctgt gaacgttgtg ctcgggattg tcctgtcagt tgtatctaca
    12661 taaccaaaga gcgtgtgcca ggcagaaagg gtttccgggt aacctcattt gtcatcgact
    12721 acaccaagtg catgttttgt gcatactgca cggagaactg cccggtggat tgcatcttca
    12781 tgggatcaac gtacgatctg agttgttatt cccgcgacgg ttgtctggtg gatttcgcga
    12841 aactccctct ggaaatcgct tggggacgtg ccagcctgaa tccaacggcg gtggccctgt
    12901 ccaagtcggt gctcgaacct gtggatgagg gtcccggagg tgcctgagct ttcgagcggt
    12961 tgatattgtc aaaaggtcga acctggttta gggtgaaggc ttacatcctt tgcttttgac
    13021 tgccgggcaa atcgacgatc tggcgaattc tgcggaaaat cacacgagca tggaatcacc
    13081 gtttaccgtg aaaaggtgtg cggatcattc acaaaccaat gcgccgatgc gaccaaccgt
    13141 ttgcggcctg cggtgatgac ggacaggcgc acttcggctt ataccggtga atttttggca
    13201 acttagcgat tggtgacgca aaaaagaagt cccaagaaaa taaaggtgac cgagaacaaa
    13261 tgtctggcgt tgctttggtt gggtacttca cagtgtttgg agccctggcc gtagggctag
    13321 tgtttggagc cttgcttttg ggcaagctct ttcggccaga taatccgaca cccatcaaaa
    13381 aagagaccta cgaatgcggt gaaccgtccg tagggtccgc ggatattcag ttcgatatcc
    13441 gcttctatgt gatggcactg gtcttcttga tctttgacgt ggaagtggcc ttgttttttc
    13501 cctgggccac cgtctttggg aaagcgacgc atgttgcgtc gattagcgtc gattctgcga
    13561 gtcgtcgcga acggctggcc gaattgggaa ttacggaagc gactttacca ccgctcagtg
    13621 gtccgaagcg cccagcgacg ttgtccaatg gtatatcagt aggtcaacaa gccgctggtc
    13681 cgacaggttt accacccgtt gaggagcttt ccgcttgggg caggcagttg gcgacactgg
    13741 cactgtgcga tatcttagtc ttttttggtg ttttgatggt tggttttgcc tatgtttggt
    13801 gtcgtggcga tttgacatgg gtacgggcgg tgcctcaccg ggcctgagca gcgatttccc
    13861 gtaccaacaa attttcgcaa gctggaacgg tcgtgcccgg gtggcagtct gatcatcaat
    13921 aagtgagcat ataccgggaa aaatgtccat cgaggagatt ttccaacacc ttcaagagaa
    13981 atttggggaa gccgtccgac caggcgactt cacttcgatt gacccgtgga ttgaggttgg
    14041 tcccgaagcg attgcggacg tttgtcgttt tttgcgcgat aatccggcct tgcggttcga
    14101 gatgctccat tgcattacgg ccgtcgatta tttggaagtg gacgaaaaaa aggctgccaa
    14161 agcctccttt gtcccccatc ttgaattgat ttatcacctc tcttcgttga cgttacgtca
    14221 ccgagtgacg ctgaaggtga tgttaccgcg gtggagagac gatcatcccg gccagcttcc
    14281 ccgcgttccc acagtcagca atgtttggaa aaccgccctc tggcacgagc gggaggtgta
    14341 tgatctttca ggcgttgagt tcgtgggcca ccccgatttg cgacgaatcc tctgcccgga
    14401 ggattgggta ggacatccgc tgcggaagga ctatcagatg ccaatcgaat accacgggat
    14461 tcgtgctcgc tagaccatgg agtgggttca gtaccatagt agtgttccat atcgtgattc
    14521 gatgggactc cttccccaag gagccacccc ctctttgaga ctgccagaca taaatatgac
    14581 agttgttacg actacaatat gaacgtgata ccaggagtcg acccggaaga tttctcggtc
    14641 acctcagaaa cagaggtgag gaccgaggaa ttgctgatca atatggggcc ccagcacccc
    14701 agtacgcatg gggtcttacg ggtcgtcctc cgtaccgacg gcgagcttgt cctcgaagcg
    14761 attccacaca tcggttatct tcaccggtgt gccgaaaaga tcggcgaaaa ggtgacgcct
    14821 cggcagttta ttccctatac ggaccgaatg gactacctgg ccgggatgaa catgaatctc
    14881 ggctgggcct tgacggtgga gaaactactg ggtctggaag tgccagaaaa ggctaggcat
    14941 ctacgtgtct tgatcgtcga actcaatagg attgccagcc accttgtggc ggcggggacg
    15001 tacggattag acctgggcag tttcactcca ttcgtatatt gctttcgtga acgggaaaag
    15061 atcttgaatc tattcgaggc cgtatgcggc gcgcgtctga cgtatagtta catcactgtg
    15121 ggtggcctta tggctgacct gccaagcggt tggctggacc agtgtgaagc gtttctcgat
    15181 caatttgaac ctgtgattga cgaccttcac gatctgcttt cggtcaatca cattttcgtc
    15241 aagcgaacgg ccggagtggg agtcctttcg cgagagttgg ccgtcgatta tggttgcacc
    15301 ggaccggtat tgcgcggaag cggtgtggat tgggatttgc gtcgcgacgg tgacccattt
    15361 tacacgcgca tgtatcgtga ttatgacttc gaggtcgccg tctgtcgcga cgggaaatat
    15421 cctcaaccct cgccaccagt cccgacagag gcggttctcg gcgattgctg gcatcgcttt
    15481 tttgtcagga tggtcgaagt ggtccaatct gtgcgactgg tccggcaggc gatcgagaag
    15541 taccgactcg cggaaggcga tattggacag cccatcaagt tgaaccaaaa gctcccgcct
    15601 ggggaggtct atctcgagac cgaatgcccc aaaggccaaa tgggattcat gattgtgagt
    15661 ctgggtgaag ccatcccttg gcgtgttcgt gcgcggagca gtagcttctg taatctttcg
    15721 gtgttgcccg aattgtgccg gggctgtttg gttgccgacg tcccggccat cgttggctca
    15781 ctcgatatcg tcttgggcga ggtggatcgt tagttcggcc gaaggtgggg tttcactcag
    15841 agaagttgta cccgaaaaga ccagggactc tggaaaaacg atggctcagt ggttgtcaga
    15901 ctggttaacg ctaagcccgc aatgggagtg ggtcgcctat ttgaccgtgg cgctgctgca
    15961 agccgtcatt ttgctggctc ttgtgatgac ggccgtcgcc ttggcaacct gggcggagcg
    16021 aaaagttgcg ggccgaattc aagatcgtct cggcccgaca cgtgtgggag gccgttttgg
    16081 atggctgcag cccctggccg acgggctcaa actttttacg aaagaagact taatccccgc
    16141 gaacgccgat gctgtgcttt ttcgattggc gccctacttg agtttcgcac cagtttttgg
    16201 cgtttttatg gcactgccgt tctctgatgg ttgggtcgcc catcatttca ataccgcggc
    16261 attcttcatc ctcgctgtgc tcgggctcga ggtctttggc attattctgg gggggtatgc
    16321 ctcgggctca aaatggtccc tattgggcgg aatgcgagag gctgcacaag ttgttagcta
    16381 cgaggtccct ctcgcctttt ccgccatgct acctattctc ttagcgggca cgatggatct
    16441 tgtggctatt ggggaggctc agcgcggctg gttttggaac tggttcgtgt tccatgatcc
    16501 ttttacattt gtggcctttt ggatctatgt gatttgcgcc gtggccggga caaatcgcgc
    16561 gccctttgat ttggctgaag ccgaaagcga actcgtcgcc ggtttccata ccgagtactc
    16621 cggttttcgt tggagcgtct tttacatggg cgaatatgcg gcgatggcag ccgtctgcgg
    16681 tttaggagtt gtcctttttt tgggtggctg gaatggtccc ataccggtgg cggagtggct
    16741 ttttggccca gccagttcga cgagtccaat tgtcaatttc ctcagccaat tgttgggggc
    16801 ggtcaatttc atcattaaat gtatcatcgg cgtcgttttc atgatctcac tgcgatggac
    16861 attgccacgt ccgcgaatcg accaggtcat gtctatgtgc tggaagtact gctttccgct
    16921 ggcagcccta gcattcctcg gggcaactct ctgggtttta atgttacctg acggtctttt
    16981 gggccaagga catcctaagg cttcgacatg tgagcaatcg gtaatggtca cggtcgaaag
    17041 gattgagaca ccatgacagg gcctgacgga atggcaatcc ttttttggtt attcgcaatc
    17101 gtcacctgcg gaggtgccct ggttgtggta ttgagtcgcc acatcgttcg ggcagccgtg
    17161 gggcttatct tgtctctttt cggcgtggcg atgctgtttt tactcgctgg ggcagaattt
    17221 gtgggagcaa tgcaactcat gatctacgta ggtgggaccg ttgtccttct cgcctttggt
    17281 gtgatgctga cggcgcggcg accgttgctg ccactatcaa ctacgcgcga ccaatggtta
    17341 gctgctgtcc tgctggcgtg ccctttgact gttatcctgg tgcaggctgc cttaagctat
    17401 gctcccaccg acagcgttag cgcgcaagtc gacgcacatt caggcgtctc aacgcccgtt
    17461 gggatgctgc ccgtaacacc ggcgcgactt ggtatggcgt tattgggttt tagccaggac
    17521 aatcaccgcc cggacggagg agaggtttca catggtgatt cgggctatct gctaccattt
    17581 gagttgattt cggttcacct gctggtggtg ctgatagggg cagcttactt ggctcgacgc
    17641 gaggtaccga gcggtatgac gccacgagat aatcagggac agttacaatg aacttccgtt
    17701 cgaggtcgta agccgagcac aatgttttcc cccataacgc ctgcccatta tttgttcgtt
    17761 ggtattgtcc tattcgtcac aggaatgatt tgcctgctga cgaagcggaa tattattgcg
    17821 atcctgatcg gtgttgagtt gattctcaac gctgcgaaca taaacttcat tgcgttttct
    17881 agtccggtac tttttccgaa acgtgttccg cccctggtct tagatggtca gattttcgtt
    17941 ttgtttgtga tgatcctagc tgccgctgag gcggcagttg cgctggccat cgcgttgcac
    18001 ttttatagcc gtttcggaac ggttgacgtt gatcaaggag atgaactgaa agggtgatga
    18061 gctcgctatc cttggtacct caactcctgc ttattgcatg gatgctgccg ttagccagtt
    18121 ttgtgttcat ctttctgacc ggctcgtttc tggaccggcg cggtcgcgct tcgggtttca
    18181 tagcgacagc tgcgatcgcg ggctcctttc ttctgtcggt cgtagcggca gccctgtggt
    18241 tcggctatta cccgctcgat tcaaatcatc aggcgagcag ccatcccatt cggccactca
    18301 gtggcgattg gtacacgctg gcggaatttg ggagcatcaa gttagccctg ggctattaca
    18361 tcgatgccct gacccttgcg atgtatgtca tggtgagctt tatcgctacc ttgattcaca
    18421 tctactcctt tgggtacatg cacgaggaac tccacgaggt caacgatccc caggtcattt
    18481 taggcgacgg cacgacgtta cggcgtcccg gccgattcgc gcggttcttt cagtacctct
    18541 cgctattcgg gttcagcatg ctggggctgg tgatcgccaa taatctcttg atgacctttg
    18601 tcttctggga actggtcggc atttgttcat actttttgat tggcttttat gtcgagcgat
    18661 ccagcgcaac ctcggccggg aacaaggcct ttatcgtcaa tcgcgtcggc gactttggga
    18721 tggttttggg cttagccgct ttgttgacga gctgcggcac actccatttc gatcactacc
    18781 aaacaacaaa tggagctcac tacgctggac ttttttctta cgctaaggcg atccagcatc
    18841 cggcatctga gctagaaccg gcggaggggt caagaagcga ggattcgcaa tcgaacccag
    18901 agacaccccc agtcacgtta agcggcccaa agggactaac gtatgggctt ttggtcttgg
    18961 ccggattggc ccttttgtgc ggatgtctag gcaaaagcgc ccagtttccc ctacatgtct
    19021 ggcttcccga cgctatggaa ggcccgacac ccgttagcgc ccttattcat gcggccacaa
    19081 tggttgcggc aggcgtctat cttgtgggga gaatctatcc cttattgact ccagaggtcc
    19141 tgatcgtcgt tgctgtcatc gggactatta cattattcat ggcggccacc attgcgctga
    19201 cggcgaccga tattaagaag gtcctagcct attcgaccat tagtcagctt ggttacatga
    19261 tgctgggtct tggaacaggt ggatgggtcg cggggttatt tcacttgttc acccatgcat
    19321 tcttcaaggc cctgctcttt ctcggatcag gatcggtaat ccatgcctgc ggcacaaatg
    19381 agatgccgca aatgggcggg cttctccgca agatgccgtg gacggcgatt acgatgctca
    19441 ttggatgctt ggcgatcgcc ggagctggta tcccgctttg ggccggattc agcggttacc
    19501 attccaagga tgccatcctc gcccaggttt acctatttca cacaacgaac cggttcctcg
    19561 gttggatcta ctacctggcc ctgttttctg ccgctttgac cgctttttac atgtttcggc
    19621 tatggttttt gacatttatt ggtcaacccc gtaatgaaca tatctttgga catgcccacg
    19681 aatcgccaaa aagtatgact gttcctcttg tcgtcctggc gttctttgcg gtggtagcag
    19741 gctggagtgt tccagggaca cactggagcc tatcggctct gctagaacaa gcccgggtca
    19801 caccgctttt ggaaaactca caggtcccac tttttggaaa cgtcgtcatc ccgcacgaac
    19861 atgaatcgca cgaagcccaa atccatcagc tggtctcctt ggaggccttt actgcaggta
    19921 tcttaggttt tgcaattgca gtggtctttt acggcgcccg ctggcttgat ccggccaagg
    19981 tctgtcagcg attcttcggg ctgtatcgtt tccttctgaa caagtggtac tttgacgaac
    20041 tttatagccg gctgtttgtg cgtcccgtga catgggtggc atcggttgct gcctggattg
    20101 accgagccgt tatcgactgg acggtcgatc atgcggccgt ggctgtgcgt tcgctagcac
    20161 atcttgacga ctggctggac cgtattctcg tcgatggcgc ggttaatcgg cttgccgcgg
    20221 ccatctacgg aatgggcgaa tggtttcgcc aatggcaaac aggccgcatt cggaactata
    20281 tcctttggat cgttgttggc ttggtaagcc tatttatttt gggatcaatt tactttgggt
    20341 tgggaatgca ccttgtcgtg ggggcttcct gatcctttac ggcatcactt ggtggaaata
    20401 ggtcatcaaa ggcacataac gggttgtcaa caatggtggc tggtttggtt ttcttaagta
    20461 ttgtggtgtt tttgcccgca ttcggcgcct tggtgttgat gttcttcccc tcgcacgccg
    20521 agcgtgccat tcgaagaaca acccttggcg taaccctggt ggtgttcttg ttgacattac
    20581 gattggtttt gccgggccta cacgcggcgt ctcagaatcc attagaacag gtccttcacg
    20641 atgtccgttt tgcggccggc gaaccaggtt atcaacacgc ttatgcggtc ccctggatca
    20701 gttctttcca tatagactat ttcctaggag ttgatggaat cagtttgcca cttgtcctgc
    20761 tgacatcttt cctttctgtc ctggcagcgg cggctagctg gtctgtcacc aaacacgtga
    20821 aggcttacag catcctcttt cttcttttag aaacggggat gctgggtgtt tttctggcac
    20881 tggatttctt tctgttttac gtcttttggg aggtcatgct tcttcccatg tactttttaa
    20941 tcggtgtgtg ggggggaccg cgaagggaat atgcggccat caagttcttc cttttcaccc
    21001 tggtgggcag cgtgttgatg ttgattgcga tccttttgct ctactttgca agcgatgtgc
    21061 gtcagcttcc cgaaaatgcc cgccgagaaa gtttccttag ccaggaagtc atggtcaagt
    21121 ccctcgcacc aagcgaaaaa cccctccaca cgttcaactt gattgccttg gccaaact
//
LOCUS       DAOSUT010000068        21159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1630_length_21159_cov_8.962472, whole genome shotgun sequence.
ACCESSION   DAOSUT010000068 DAOSUT010000000
VERSION     DAOSUT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 21159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21159
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_21159_cov_8.962472"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLD05_07970"
     CDS             complement(<1..707)
                     /locus_tag="PLD05_07970"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOP77414.1"
                     /translation="MSAGNDRPLIAITTGDPAGIGPEIIVAAWSDPRVHGICRPVVVG
                     YSEYLRRAAALRRSNVQIETCDDPQKARPSPNVIFCVAPGEDRAPPVQVGRIDPRAGQ
                     AAFEAILLAAELALAKKVDGLVTAPINKEALHAAGHQYPGHTELLADLCQRQQVAMVL
                     YLGPGAPLRGTAGLAIAHATLHIPMREVIHQLTAEAVFTKIRLIDQLMRCIKGSEPRI
                     GVCALNPHAGEGGLFGR"
     gene            948..1421
                     /gene="dtd"
                     /locus_tag="PLD05_07975"
     CDS             948..1421
                     /gene="dtd"
                     /locus_tag="PLD05_07975"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOP77415.1"
                     /translation="MKACIQRVRWARVRVNGETVGEIGPGLLVLLGVAMDDTEQEARM
                     LAEKIVGLRIFEDEAGKMNRSVGDAGGSLLVVSQFTLLADCRRGRRPSFTDAAPPDRA
                     NMLCEYFIQQVRKTGLPVATGRFREHMEVELLNDGPVTIVLDSTELKSPRREHGG"
     gene            1507..2064
                     /locus_tag="PLD05_07980"
     CDS             1507..2064
                     /locus_tag="PLD05_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOP77416.1"
                     /translation="MVDLGIPIYDLVMGAVVLFSIVYGMSRGFIWQLASLSSLILSCW
                     AAVRWSPNLARIISHQEPWNRWVAMLLIFVFCSLIVWLIFQVVGKWLRRVHLEGFDRQ
                     MGAVFGLIKGILFCLVITFFSVTLFTSTRSLVLTSRSGPIIARLIPKVAVLLPKEIRT
                     SVGEYLEEFETQLQAPPPDSTIPDN"
     gene            complement(2345..>2824)
                     /locus_tag="PLD05_07985"
     CDS             complement(2345..>2824)
                     /locus_tag="PLD05_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOP77417.1"
                     /translation="QVIAESMFLMSQLPTPIICVVIGEGGSGGALAIGVGDRVAMLEH
                     AYYSVISPEGCAGILWKDRQYKEEAARALKMTSKDLIQLGLVDHVIPEPLGGAHRDPF
                     QMASRLKLYLRNTLKELMSIPIPELVEARYQKFRRMGVYLELNEAEPNPTDQVPSAG"
     assembly_gap    2825..2924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2925..3121)
                     /locus_tag="PLD05_07990"
     CDS             complement(<2925..3121)
                     /locus_tag="PLD05_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOP77418.1"
                     /translation="MATEFRLAFERPIYELESRIEKLERLAQTDPNVREELQQKRLEL
                     VELKKQIYSNLKPWEVVEVAR"
     gene            complement(3183..4061)
                     /locus_tag="PLD05_07995"
     CDS             complement(3183..4061)
                     /locus_tag="PLD05_07995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP77419.1"
                     /translation="MSTAAVTYIGRYRLVNPVYLGATTQLWQAFCDSTGQFVALKLLL
                     EKFRRDREQIRSLKWEYTIASQFDDPRIIKIFEFNIDRGIPYLAMEWFNGGNMKNLIR
                     RGIEEYAPFVRKIIFQCIEAVSYLHRQGFVHRDIKPENFLVSPQGDVKLIDFALCRKI
                     GPKILWLFRPKSKIQGTCSYMSPEQILNKPLDGRADLYSLACTIFELVCGKPPFTGMN
                     QTELLNKHLKSNPPPLEAYNPNVHPEFSRIIQKALAKRPSDRFKSIGEFAGALYDVPV
                     FKVPPPDEVPVQLTLA"
     gene            complement(4184..5359)
                     /locus_tag="PLD05_08000"
     CDS             complement(4184..5359)
                     /locus_tag="PLD05_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOP77420.1"
                     /translation="MRVLHIITRLIVGGAQENTILTCEDLIHRYHDDVLLVTGPPLGP
                     EGSLLERARTGGIPIKLVPSLRRAINPWHDLRAYLAIRKSIKEFRPEVVHTHSAKAGI
                     LGRAAAWQLRVPVVVHTVHGAPFHPYQPAVAQKFVIACEKWAAAHCHAFISVADAMTD
                     LMVNAGIASRERFTTIYSGMDVKPFLDCDKHRAEMRCKLGYDEQDVVIGTIARLFHLK
                     GHEDILAAAETIIPKFPQVRFLWVGEGVLRPRLEAQIRSRGWEGRFHLAGLVPPDDLP
                     RWLSAMDVIVHTSLREGLARVLPQALLSGKPVVSYDIDGAREVVIPGQTGYLIPPRNL
                     SGLIAALEELVQTEHLRQTLGKRGQELCSQEFDRYKMTERIRALYVRLLKEQGKAQG"
     gene            complement(5495..6331)
                     /gene="truA"
                     /locus_tag="PLD05_08005"
     CDS             complement(5495..6331)
                     /gene="truA"
                     /locus_tag="PLD05_08005"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOP77421.1"
                     /translation="MRCIRLTLAYDGSNFLGWQRQANGRTVQGVIEAAIEKISGKRSR
                     VLASGRTDAGVHALGQVAAFRTESTLTAETWGKALNAVLPHDIVVLAAEDVPLSFHPI
                     RNAQRKRYCYLLDDGAVRPVIFRQFVWHYPRPLDVDLMRTASEFLIGQHDFAAFETSG
                     APRKTTVRTIFELSVRRVHPYMPSVIAIEIEADGFLYNMVRNIVGSLVEIARGARPVA
                     WLGEVLASRDRRLAGPTAPPQGLFLVSVTYDNLPTFSAANPWFLGSLRMVHAENETRL
                     EQ"
     gene            complement(6358..>7329)
                     /locus_tag="PLD05_08010"
     CDS             complement(6358..>7329)
                     /locus_tag="PLD05_08010"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOP77422.1"
                     /translation="AVGRIMVQLLERSSLTFERIKFLASARSIGKTIEFRGEKYPVQE
                     LRPEAFDGVDLAVSSTPDEVAKEFIPEAVRRGCVVVDESAAWRMDPNVPLVIPEVNPH
                     AAFKHNGIIASPNCSTTQMVVALKPLHDYGRVRRVIVSTYQAASGAGVGGTKDLFEGT
                     RAYLENRPYAYTTFAHPLAFNLIPQIGSPRPNGYTSEEMKMVLETRKIMEDESIQICA
                     TCVRVPVANSHSESILVETEKKITVEKARELFSKFPGIVVIDNLEAKQYPLPLNCSGR
                     DEVFIGRIREDFSSPNGITFWCVSDNLRKGAATNAVQIVELLLRGRP"
     assembly_gap    7330..7429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7518..8303)
                     /locus_tag="PLD05_08015"
     CDS             complement(7518..8303)
                     /locus_tag="PLD05_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03000 domain-containing protein"
                     /protein_id="HOP77423.1"
                     /translation="MRPRILMAFAALGIGVGCLGATSEACWPRHVCGPWYPGPLTALA
                     CWAPCWSVCDVCQPYDPCCYDDGWVLGVRPGPIRRLILGPYRWYRVPFGCCDVCGEVA
                     CTCEPAIVTGPALTPTPAQPFSTPAQPTPAQPSPTPDALPSPSPTPGADIPNQPAPST
                     PLVNPTSYTVPTRNDSGLLTVYVPAEAKVLINGLPTKSTGSRREYVSYGLKEGLQYRY
                     VITAQVEQDGKLYEETREVILTAGEKKGVAFSFQFPTENIAGL"
     gene            complement(8608..9852)
                     /locus_tag="PLD05_08020"
     CDS             complement(8608..9852)
                     /locus_tag="PLD05_08020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOP77424.1"
                     /translation="MKTWFQRLSFVVIVVAFSPVSGRAEQGGRGILPFDRLRQEMVRR
                     DILGGGIKNPRVIAAMASVPRHEFVPPEQRRLAYHDMALPIGYGQTISPPFVVAYMTE
                     QIDPQPTDRVLEIGTGSGYQAAILSGLVAEVYTIEIVEPLARQAEATLRRLGYKNVFV
                     KAGDGYQGWPEHAPFDKIIVTCSPEKVPQPLIDQLKEGGRMVIPVGERYQQTMYLLKK
                     TGGELVKESLRPTLFVPMTGIAESQREVKPDPAKPQLINGDFEEIRPDAPEPPGWYYC
                     KQFELRQNDGAPSGSVYLALRNREPGQPARVLQGFPIDGRQVQEILVTFFVRAQNVRP
                     GRNPQESAGVIITFFGEDRAPVGEATIGPIMGTFPWRHVSRRIQVPAAAREAIIGIGM
                     TGAVGELAIDRVEILATSEKKP"
     gene            10149..>10657
                     /locus_tag="PLD05_08025"
     CDS             10149..>10657
                     /locus_tag="PLD05_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOP77425.1"
                     /translation="MVNNADLRDKVIRKVLPRVQTPAQYLGGELNSVVKDTSQVCGHL
                     CLCTPDTYAIGMSNHGLQVLYDTMNRRTSWACERVFAPGRDMEAELRHWNLPLYSLET
                     FTPLYRFDVIGFSLQYELSYTNVLTVLDLGRIPLLAEERDLNDPLVIAGGPCAQNPEP
                     MSCFIDLFV"
     assembly_gap    10658..10757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10758..11648
                     /locus_tag="PLD05_08030"
     CDS             <10758..11648
                     /locus_tag="PLD05_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOP77426.1"
                     /translation="AAEEAYRNTGTNEIALLSLSSSDYPQFAELITRLREVFAPRHVA
                     ISVPSLRVNEQLRVLTQMLTPDRHSGLTLAPEVALNDMRVQIGKRIRNEDLIEGCREA
                     FRSGFDRVKLYFLCGLPGEREADLAGIVELAQEISGLGRSILGRPATVVANVSNFVPK
                     PHTPYQWHPMQTREYFTQAHQFLRQRCRMRSVQIKCHPVESSLLEGIMTRGDRRMGQA
                     ILEAWRRGARFDGWAEQFNPQIWWDVFDDLELPVEETLHRPWSPEARLPWDHVSIRQG
                     RGYLERERDRAWRDLAALNR"
     gene            11890..12735
                     /locus_tag="PLD05_08035"
     CDS             11890..12735
                     /locus_tag="PLD05_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOP77427.1"
                     /translation="MGYRFLALDIDGTLVSTVDAISPSVREAIARAAEAGLEVILATG
                     RRYSRALPFSKILQIETPLVTSSGALTKIPKTHETLFRARFSDEVLRKTLMVLRELGF
                     PAAILGDTFSHGFDYYIERPNGYRNPYFEDYLEQNSGSERIWDDFLAELPPEIFATFT
                     VGSEAEMKWVECQLHAALPHQLTTNVLRSPRYRGFFCEIMPAGTTKWSAICQLAAQRG
                     VTPREICAVGDDVNDISMIREAGFGVAMGNAPDEVKKAAHWVAPPQEEDGLVAVIERL
                     LEESS"
     gene            12782..13129
                     /locus_tag="PLD05_08040"
     CDS             12782..13129
                     /locus_tag="PLD05_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOP77428.1"
                     /translation="MSETHETIPEETGDRDITFSDFQRLIRKMYFEKDVARGVEGTFM
                     WLVAELGELAEALRSGTAEEKAEEFADVVAWLTTIANVSNVDLTAAIRKKYGQGCPGC
                     GRFVCVCPDAGKP"
     gene            13207..15462
                     /locus_tag="PLD05_08045"
     CDS             13207..15462
                     /locus_tag="PLD05_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77429.1"
                     /translation="MDYWREQTATVQTAFSKSMKQALILFLELLVWFSSIAAVCADGE
                     TTAPEIVNLRVGVEGVYKLGLWTPVWVELRGGTGEASGTIVVTVPDDEGGPCSYVMET
                     VTLPAQATREVEMLVQIGRPRAQATVRWEAEGGPSVEKVFQAGTASFPTAIAARTPVF
                     LWVGPSQPPQALMGRRNEKGHWVWVKNPERLPTHFLGYQSVRTVVLAIGDDKEFIGSL
                     QRSGKQMLALQEWVREGGKVVASFGPTAKTVVSDGGVLSWVAPGTWQNAIPLRKCAGI
                     ETYVDSPRPIVVRRSDPPLQALQFTNFEGIVTASEGNIPLVIRRQYGFGTIVFFTTDL
                     THRALVEWTQFDRLIDRLVGYSVAQDDVAGNPPRTLMHYGYTDVSGQLRSALDVLPTI
                     SFVPFSWVAVLVVIYLVIIGPVDYWILGYGIRNYRWTWITFPAIIILAAGAGILLGYS
                     KGERPVTKEVHWIDYDLAGSTIRGLGLGSLYSLHPDFYDISFTAPDFGTKSEVGQLWL
                     GWFGIPGHGLGGMENTTGVLSSLEPYTAMPRSGLLQGFPVNQWSTRSVWALWSAKSQT
                     PPLQLAVFDREGVLRGRIQNNLDVTLENCFVAYGDWVYDLGTLEPGQAWESFLGDDRR
                     ELRSWLNGRQVFVEGEGVRAQTRQRVQPYETGSRDVGYILRMMAFYQAAGGLGYVELS
                     HEYWPFFDGSEWLANGRAVMIARVRTERQASPLFGVTARNQTFESVTPVAQECFCRFV
                     LPVPKGPPLAE"
     gene            15525..16538
                     /locus_tag="PLD05_08050"
     CDS             15525..16538
                     /locus_tag="PLD05_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP77430.1"
                     /translation="MITKKTVDKQVGVLPQGQTDSDRKSSRAVAVKTVGLTKLYGQIR
                     AVDQLTLELEEGDLFGFIGPNGSGKTTTMRILATLLQPSWGEAYICDHSIYTHPKEIR
                     RLIGYMPDFFGVYDDMKVIEYLEFFASAYRINGPKRRKVCEEMLELVELGYKRDAFVT
                     SLSRGMTQRLGLARVLLHDPQVLILDEPASGLDPRARIEIRELLKRLRQMGKTIMVSS
                     HILPELADICNKIGIIEQGVLKVCGDLKEVMQRVREQKVLCVEVWEKQAEAVRILESQ
                     DDVHSVEVRDGVIYVTLKRGVNDYRDLATALVQGGCPLKMLREDEINLETAFMALTRG
                     MTA"
     gene            complement(16562..17350)
                     /locus_tag="PLD05_08055"
     CDS             complement(16562..17350)
                     /locus_tag="PLD05_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOP77431.1"
                     /translation="MKKNAVKLALREGRTVWGTWVTIPDDRVVRILAEIGFDWVTVDM
                     EHTALDWSQVDRLVGWAIDAGTTPLVRVPAGDPVYIKRALDLGAFGLVIPMVESAEQA
                     RQIIAASQYPPQGSRSAAAANHYRRFQTTLEKYLAAANEQIMIVLQIESPAGVEEASN
                     IAGIPGCDALLIGPLDLACRLCKPPQRLPQASKEDFEAAVEKVIEAGRQRHCPTGFHA
                     RTVDEAQHWAARGMQLLAVSNDLTFLATQAGDVLHRLKSGIGRG"
     gene            17602..17940
                     /gene="nirD"
                     /locus_tag="PLD05_08060"
     CDS             17602..17940
                     /gene="nirD"
                     /locus_tag="PLD05_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOP77432.1"
                     /translation="MDLFDWQSSGEWIPVCKLDDLQKNKGKRFVVAGREIAIFRTENG
                     VFATDDYCPHMGDSLSAGRIWEGTVVCPRHMWAFRLADGRCTDVPTLSVPTYKVTVRD
                     GWVLLKKPVE"
     gene            complement(18029..18715)
                     /locus_tag="PLD05_08065"
     CDS             complement(18029..18715)
                     /locus_tag="PLD05_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOP77433.1"
                     /translation="MATQLRWLGHGCWLIETNGHRIILDPFLDESPVAPIKADEVKAD
                     YVLISHGHFDHMADAESIAKANAATVIATYEICEWLNRRGVTSTHAMNVGGTHAFPFG
                     RVKMTPAVHTSMLPDGSYGGVAVGFLLLLQDGNVYFACDTGLFLDMQLIGDHDLALAV
                     LPIGDNFTMGPEDALRAVTLLRPKRVVPVHFNTWPLIAQDVNDWANRVRHTTASVPVV
                     LQPGESIALG"
     gene            complement(18834..20264)
                     /locus_tag="PLD05_08070"
     CDS             complement(18834..20264)
                     /locus_tag="PLD05_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOP77434.1"
                     /translation="MRQSEISGLSLRYECAGMEVPPERPAIERETGVLTEDLEWLWPR
                     LLACRDEVLDEVELKKRGDKVPKEKEPLDSRFIDLPERLLGENRTVLEHIRQTSERFA
                     SGLERVVVLGIGGSYMGARALFEACCHPYHNELSRSQRGNRPRLYFEGNNVDNDAVLG
                     LLELLHSEPTADWGIVVISKSGSTLETAVAFRVFVEALKKHCQDRGVKVGQRILAVSK
                     EDSRLWRLCVNLGVPRDHMFAFPEDVGGRFSVLSAVGLVPAAILGLNIEELLEGACAA
                     NRAFSNSDPASNPILRYVGVCHLMESRRGASIRVLSTWGKRLEAVGLWYDQLLSESLG
                     KNGQGATPLTVVNTRDLHSRGQQHQEGRRDKLFTNLWAERPSLACVTIPRWDYDDDQL
                     NKLAGKSLSDILRAARLGTTQAYAEDLRRSAEIILPQIRERTLGELFQFLMLATVVEG
                     RLIGINPYGQPGVEAYKRHMQKLLEQ"
     gene            complement(20449..>21159)
                     /gene="phoU"
                     /locus_tag="PLD05_08075"
     CDS             complement(20449..>21159)
                     /gene="phoU"
                     /locus_tag="PLD05_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOP77435.1"
                     /translation="KDLDMAVHMHREMDRLRERLLRLCGEVEGQLHKAIEAVLKRDPQ
                     LADEVESSDRKIDAAEVDIEEECLTILTLHQPVAADLRFLVSVLKINNDLERIGDLAV
                     NIARKARTLSQYPPIRLPFDLSEMWGKAQVMLRNSVDSLVNCDLDLAIDVCRQDEAVD
                     AQKRMIREHVERMIQETPDLVRPLLQVLAISRNLERVADHATNIAEDVIYMVRGQIAR
                     HAGPTVPSKEDPTKGQIA"
BASE COUNT         4571 a         5625 c         5907 g         4756 t
ORIGIN      
        1 tccctgccga acaagcctcc ctcccccgca tgagggttta gggcgcagac accaatacgg
       61 ggctcactcc ctttgataca ccgcatgagc tgatcgatga gccgtatctt agtgaaaacg
      121 gcctccgcag ttagttggtg aatcacttcc cgcattggaa tatggagcgt cgcgtgagca
      181 atggctaacc ctgccgtccc ccgcaagggc gcgcccggtc ccaaatacag aaccattgcc
      241 acttgttgtc tttgacataa atcggcaagc agttccgtgt gtcccgggta ctgatggcca
      301 gctgcatgga gggcctcttt attaatcggt gccgtaacaa gcccatccac cttcttggcc
      361 agggccaatt cggcagccag caggatggcc tcaaaggctg cctggccagc gcgtgggtcg
      421 atgcggccca cctgcacggg tggtgctcgg tcttccccag gagctacaca aaaaatgaca
      481 ttcggtgagg gtctggcctt ttgaggatcg tcgcaggttt cgatttgtac gttggaacgt
      541 cgcagggcgg cagcccgccg gagatactcc gaatagccaa ccacgacagg gcgacagata
      601 ccgtgcacgc ggggatcaga ccaagcggcc acaatgattt ctggaccaat tcccgcaggg
      661 tccccggttg taatggcgat tagaggacga tcatttccgg cgctcatggt tgttatcctc
      721 tgtctcgggc aggagaaaaa tgatttttgt accctctcgg tgatcaggga cctctttgcc
      781 cgctatttta aggtcgccct tcaccgttga gcaaatactt tctgccacaa aatcggtgtt
      841 tccgcccctt cgagagacat tctatgatat taaaggcaga tcgcgcagat atgccatcaa
      901 aaaaccaggc ttggtttgga gaagtcaaat ctattgagca ccttcgcatg aaagcatgca
      961 ttcaacgtgt tcgctgggca agggtccgtg taaacgggga aaccgtcggc gagatcggcc
     1021 ccggattgct ggtactcctc ggcgtggcca tggacgatac cgaacaagag gcacggatgc
     1081 tcgccgagaa gattgtcgga ttgagaatat tcgaagacga ggcgggaaag atgaatcgct
     1141 cggtgggaga tgccggcgga tccctcttgg tcgtgagcca gtttaccttg ctggccgatt
     1201 gtcgtcgcgg gcgccgtccc agcttcacgg atgcagctcc cccagatcgc gcgaatatgc
     1261 tttgcgaata tttcattcaa caggttcgca aaacgggttt gccggtagcc acagggcggt
     1321 ttcgggaaca catggaggtc gagctcctca atgatgggcc ggttaccatt gtgcttgaca
     1381 gcaccgagtt gaaatcgccg cgacgcgaac acggcgggta atggttcgtc ggtggggttg
     1441 ctaaagaatt acagaatgca cggaaagccg cagcagatac ggtcaaggtg gaggtcttga
     1501 agaaccgtgg ttgatctcgg cataccgatc tatgacctgg ttatgggtgc cgtcgtgcta
     1561 ttctcgattg tgtacgggat gtcgcgcggc tttatctggc aattggcctc gctctcatca
     1621 ctcatcctta gttgctgggc tgccgtccgc tggagcccca atctcgcccg gattatcagt
     1681 caccaggaac cttggaatcg ttgggttgca atgcttctta tcttcgtttt ctgttctctg
     1741 atcgtgtggc tcatctttca ggtagtgggt aaatggctta ggcgggtcca ccttgaaggt
     1801 ttcgaccgcc aaatgggtgc agtgtttggg ctaatcaagg gcattctgtt ttgtcttgtg
     1861 ataacttttt tttcggtaac actgtttaca agcacccgat cccttgtcct cacatcacgc
     1921 tctggtccga tcatagcacg actgatcccc aaggttgcgg ttcttctccc caaagagatt
     1981 cggacctcgg taggggagta cctcgaggag ttcgagaccc aacttcaggc gccgcccccg
     2041 gactcaacga ttcccgataa ctgaaaagcc agcccgtgcg agcgctgcaa cagcgggcga
     2101 gggccgatgg tcgccataga acttggggca acacgtcact tggaccggca tccaacgatg
     2161 actgaacggc caagagtgtc gcggcaacgt gcaacttttg agaaatgaag aatgctgccc
     2221 aaaaaccgga cttccgaccg aacataagaa acattatcgg acgttccagg tcgctgaaaa
     2281 ctggccccaa ccgctggttc tcgccccggt tgtcgccgaa cgtgctcctt gggcttgtcg
     2341 gcattcagcc ggcgctgggc acctgatctg tcgggttagg ctcggcttcg ttgagttcca
     2401 aatacacgcc catgcgacga aacttctgat atcgagcctc gacgagctcg gggatcggaa
     2461 tgctcatcag ctccttgagc gtgttccgga gataaagctt gaggcgagat gccatttgga
     2521 acggatcccg gtgggcaccg cccaagggct ccggaatcac atggtcgacg agtccaagct
     2581 gaatgagatc tttggaggtc atctttaggg cgcgggcagc ttcctctttg tattggcggt
     2641 ccttccagag gatgccggcg cagccctcgg gactgatcac cgagtaatag gcgtgttcca
     2701 acatggccac acggtcaccc acgccaatgg ccaaggcacc gccggaaccc ccttcaccga
     2761 tcaccacgca aatgatgggc gtgggtaact gggacatgag gaacatcgat tcggcaatca
     2821 cttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgccga gcgacttcca
     2941 cgacctccca aggctttaag ttactgtaga tttgcttctt tagctcgacg agctcgagac
     3001 gcttttgctg caactcctcg cgaacgttgg gatctgtctg agccaatcgc tcgagttttt
     3061 caatgcggct ttcaagctcg taaatgggac gctcaaatgc gaggcgaaat tcggttgcca
     3121 tggcttccac ttcctgtctt agtcataaga tgggtggcgt atcgctttta gtagttacac
     3181 agctacgcca gcgttaactg gaccgggact tcatcgggcg gcggtacttt gaaaacaggc
     3241 acatcgtaca atgctccggc gaactcgcca atcgacttga aacgatcgga ggggcgtttg
     3301 gccagggctt tctggatgat gcgggagaac tcgggatgca cgttcgggtt atacgcttct
     3361 aacggaggcg ggttagactt caggtgctta ttcaggagtt cggtttgatt catcccggtg
     3421 aatggtggct tgccacagac caattcaaaa atcgtacagg ctaggctgta gagatcggcc
     3481 ctcccgtcta agggcttatt tagaatctgc tcgggggaca tataactgca agttccttgg
     3541 attttcgatt tggggcggaa caaccaaaga atcttcggcc ctatttttcg gcacaaggca
     3601 aagtcgatca gcttaacatc cccctgtgga gaaacaagga aattctctgg tttgatatcg
     3661 cggtggacaa aaccctggcg atggaggtac gagacggcct ctatgcattg aaagatgatc
     3721 ttccgcacga acggggcgta ctcctcgatc cctctgcgga tgagattctt catattgccg
     3781 ccattgaacc actccatggc cagatagggt attccgcgat cgatgttgaa ctcgaagatc
     3841 ttaatgatgc gtggatcatc gaactggctt gcgatcgtat attcccattt caggctacgg
     3901 atttgttcgc ggtcgcggcg aaatttttct agcaataatt tgagcgcaac aaattgacca
     3961 gttgaatcgc agaatgcctg ccacaactgt gtcgttgcgc ccaaatacac cggattgacc
     4021 agtcgatatc gcccaatata tgtaactgcc gcagtactca cgctcgcacg actcgcttca
     4081 attagttcat atcacccagt ttgctcattt tgcccacaac ctatttcccg tacgatcctt
     4141 gtgatcatct taacgatgtg gaccagcaaa ctcaaccaca aatttacccc tgggccttac
     4201 cctgctcctt caacaagcgg acgtacaacg cacggattct ttcagtcatc ttgtagcgat
     4261 caaactcttg cgagcaaagc tcttggcccc tcttgccaag tgtctgcctt aaatgctcgg
     4321 tttggacgag ctcctcgagg gccgctatga gtcctgacag gttacgcgga ggaataagat
     4381 agcccgtttg gcccggtatg acgacttcgc gggcaccgtc gatatcgtaa ctcaccacgg
     4441 gctttccgga gaggagcgct tgcggcaata cccttgccag gccttcacga aggctggtat
     4501 ggacaatgac gtccatcgcc gagagccagc gaggcaggtc atcgggcgga acgagacctg
     4561 ccaaatgaaa ccttccttcc caaccgcggc tgcgaatctg tgcctctaaa cgtgggcgaa
     4621 gaaccccctc acccacccag agaaaccgca cctggggaaa cttcggaatg atggtttccg
     4681 ccgcggcgag gatgtcttcg tgccccttca ggtgaaacaa ccgcgcgatt gtcccgataa
     4741 ccacatcctg ctcatcgtaa ccgagcttgc aacgcatctc agcccgatgc ttgtcgcaat
     4801 caagaaaggg cttgacgtcc atcccactgt agatcgtcgt gaaacgttct cgagaggcaa
     4861 tgcctgcgtt gaccatgagg tccgtcatag catcggccac actgatgaag gcgtggcaat
     4921 gcgccgccgc ccatttttcg caggcgatga cgaatttttg cgcaactgct ggctggtagg
     4981 gatgaaacgg ggcaccgtgt accgtatgaa ccacgaccgg aacccgtaac tgccaagccg
     5041 ctgcccgacc caaaatcccg gctttcgcgc tatgtgtgtg aacgacttcc ggtcgaaact
     5101 ccttgatact ctttcggatt gccaagtagg cacggaggtc gtgccaggga ttaatggctc
     5161 tccgtaagga cggcacgagc ttgatcggaa tcccccctgt cctagcccgt tcgagaaggc
     5221 ttccttcagg tccaagtggt ggcccggtca caagtaagac atcatcgtgg tatctatgaa
     5281 tgaggtcttc acacgtgagg atcgtgtttt cttgtgcccc gcctacgatc aaccgtgtga
     5341 taatatgaag gactctcata acgagctgcc tgtcgttgag caacgattgc gtgctgactg
     5401 tagaggagag agcggcatct ggtttcggcc ctctgaaaac actaaatatg tttcgcagta
     5461 tgccataacg atcgtgtcat accccggtga gcccctattg ttcaaggcgg gtctcgtttt
     5521 ccgcgtgcac cattctcaag gagccgagga accacgggtt tgcagccgaa aaggtcggca
     5581 aattgtcgta ggtgaccgat accaaaaaca ggccttgggg tggggcggtt ggtccggcaa
     5641 ggcggcggtc tcgagaagcc aagacctcgc ccagccacgc cacaggtcgg gcaccccgcg
     5701 caatctctac gaggctcccg acaatattgc gaaccatatt atacaaaaaa ccatctgcct
     5761 caatctcaat cgcgatgacc gaaggcatat acgggtgcac tcggcgaact gagagttcga
     5821 aaatggtgcg gacggttgtt tttctagggg caccgctcgt ctcaaaagcc gcaaagtcgt
     5881 gttggccaat tagaaattca ctggccgtgc gcatgagatc gacatcaagt gggcgaggat
     5941 agtgccaaac gaactgccgg aaaatcaccg gacgtacggc accgtcgtcg aggagataac
     6001 aatagcgttt tcgctgagca ttgcggatag gatggaagga caacgggacg tcctcagccg
     6061 caagaacaac aatatcgtgg ggcaagacag cgttgagggc cttgccccac gtttcggcag
     6121 tcaatgtgct ttccgtccga aatgcagcga cttgtcccag ggcatgtacc ccagcatcag
     6181 ttcgaccact tgccaggacc cgagatcgtt ttccggagat cttctcgatg gcggcctcga
     6241 taaccccttg gacggtccga ccgtttgctt gacgttgcca tcccaggaag ttgctgccat
     6301 cgtaagcaag ggtcaacctg atgcatcgca tgggacctgg tccgggcgga acccgcgtta
     6361 gggccgtccc cgcaacagaa gttcgacaat ctgtacggcg tttgtcgcgg cacctttgcg
     6421 gagattatcg ctcacacacc agaacgtgat cccgttcggc gaagagaagt cttcccgaat
     6481 acgaccaatg aagacctcat cccggccaga acagtttaaa ggcaatggat attgtttcgc
     6541 ttccaggtta tcaatgacga caatccctgg gaatttcgag aaaagttccc tggccttctc
     6601 aacggtgatc ttcttctctg tttctaccag gatcgactcg ctgtgagagt tggccacagg
     6661 cacgcgaacg catgtcgcgc agatctgaat agactcatcc tccatgatct ttcgcgtttc
     6721 gaggaccatc ttcatttcct cggaggtgta gccgttgggc cggggagacc caatttgggg
     6781 aatcaggttg aatgctaaag gatgggcaaa ggttgtgtac gcgtaaggcc tattctccaa
     6841 ataggctcgt gtcccttcga aaaggtcttt tgtcccaccg acaccggccc cacttgccgc
     6901 ctggtaggtg ctcacaatga cgcgtcgaac cctgccgtag tcgtgcaacg gtttgagggc
     6961 caccaccatc tgggtggtgg aacaattggg gctagcgatg atcccgttgt gcttgaaggc
     7021 cgcatgggga ttgacctcgg ggattaccag aggcacgttt gggtccatcc gccacgcagc
     7081 gctttcatcg acgacgacac atccccgccg gacggcttcg ggaatgaatt ctttggccac
     7141 ctcgtcgggc gtgctgctaa cggccaaatc aactccgtcg aaggcctccg ggcgaagttc
     7201 ctggacggga tatttttctc ctcgaaactc aatcgttttc ccgatcgagc gcgctgaagc
     7261 aaggaatttg attcgttcaa acgtgagaga acttcgttcc aaaagctgga ccatgatccg
     7321 gccaacggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggacagcgtc
     7441 gttttctaat gagcttccta atctttcgat ctttctttgg cttgccaacg aggggctctt
     7501 atccaacctg cttctgatca aagacccgcg atattctccg ttgggaactg gaaggagaac
     7561 gcgaccccct tcttttctcc cgccgtgagg atgacttcgc gggtttcttc gtacagtttc
     7621 ccgtcctgtt ccacttgggc cgtaatgacg tagcgatatt ggagtccttc cttcaaccca
     7681 tacgagacgt attcccgacg gcttcccgtg ctcttggtcg ggagaccgtt gataaggacc
     7741 ttcgcctccg cagggacata gaccgtgagt agaccactgt cgttccgtgt ggggaccgtg
     7801 tagcttgtgg gattgacaag cggcgtcgat ggggccggct gattagggat atcggcgccg
     7861 ggagttggac tgggtgacgg aagggcgtca ggggtcgggc tcggctgagc tggtgttggt
     7921 tgcgccggtg tggagaaagg ttgagctggc gtcggcgtca gtgctggccc tgtgacaatc
     7981 gccggttcgc atgtacatgc gacttcgcca cagacgtcac agcacccgaa aggaacacgg
     8041 taccagcgat acggaccaag aatcaaacgg cggatggggc cggggcggac gcccaggacc
     8101 caaccatcat cgtaacagca tggatcatat ggctgacaga cgtcacaaac cgaccaacat
     8161 ggcgcccagc aggccaaagc ggtaagaggt cccggatacc atggaccgca gacatgacga
     8221 ggccagcagg cctccgatgt cgctcccaaa caccctacgc caatccctaa cgcagcgaag
     8281 gccatcagaa ttctcggtcg catcgtttgg ttcctttcgc aagaaggtcg gccgttccgg
     8341 ctaagtgaca ccgcgtcctg agaaaaccta cgaaatcttt gtcactttta tccaccttac
     8401 cctggcagga acggggattt caagtggtcg ggcaaagttt accgattatg cgggctatgt
     8461 cgggctatac cctcccgcga ggtaacgcac gttgaagcgt aatagcatac ccgcgtgaaa
     8521 aaaacgacag cccagtggtt gctaactttt ctgatcggtg gaaatcaccc aagggctttg
     8581 caccattccc cgttgccgtt gtaattgtta tggcttcttc tcggaggttg ccaggatttc
     8641 gacgcgatcg atggctagct cccccacggc acccgtcatg ccgattccga tgatcgcttc
     8701 ccttgccgcg gcggggactt ggattcggcg cgatacatgc cgccagggga aagttcccat
     8761 aatgggaccg atcgttgctt ccccaacagg tgccctatct tccccaaaaa aggttataat
     8821 aacccccgcc gactcctgag ggtttcgacc agggcggacg ttttgtgctc ggacaaaaaa
     8881 tgtcactagg atctcctgca cctggcgccc gtcaatggga aatccctgaa gaaccctggc
     8941 cggctgcccc ggttcccgat ttcgcagggc cagataaaca cttcctgaag gtgcgccgtc
     9001 gttctgtcgg agctcaaatt gcttgcagta ataccacccg ggcggttccg gggcatccgg
     9061 gcgaatctcc tcgaaatctc cgttaatcaa ctgcggcttt gccgggtctg gtttaacttc
     9121 gcgttgagat tccgcgatgc ccgtcatagg gacgaacaac gttggcctga gtgattcctt
     9181 cacgagttct ccgccggtct ttttgaggag atacattgtt tgctggtacc gttcccctac
     9241 cgggatgacc atccgcccac cctctttaag ttgatctatc agtggctgag ggaccttttc
     9301 cggtgagcag gtgacaatga tcttgtcgaa tggggcatgt tccggccacc cctggtaacc
     9361 gtcacccgct ttgacaaaaa catttttgta tcccagtcgc cggagggtgg cctcagcctg
     9421 tcgggcaagg ggttcgacga tctcaatcgt gtaaacttcg gccaccaaac cactgaggat
     9481 cgcggcttga tagccacttc ccgttccgat ttccaacacc cggtcggtcg gttgtgggtc
     9541 aatttgctcc gtcatgtagg ctacaacaaa gggcggagag atcgtttggc cgtacccaat
     9601 cggaagggcc atgtcatgat aggcgagtcg gcgctgctcc ggcggcacaa attcatgacg
     9661 cggaaccgat gccatggcag caatgacacg cgggtttttg atcccacccc cgaggatgtc
     9721 gcgacgaacc atctcctgac gaagccggtc aaacggcaga atgcccctgc ccccctgctc
     9781 tgctcgacct gagaccgggg agaaagccac cacgatgaca acaaacgaca aacgttggaa
     9841 ccaggttttc atgctaccca cccgatccat tgatcacggt tataccatct cccctcggtg
     9901 gcctcattga gggcgttcaa cttttgccaa gcggcccaat cactttggtc atgtcccaat
     9961 ggctgaccta tgttgctcac ctcacttcca ttgtagttac tcgtcttcgg tttcatccac
    10021 gaagaaaggg actctcccag gtaagttccg gttgaagata gcggtgtttg actcaagagc
    10081 gttttccaca acaatgaggt aaggggtctt cgcaaggctg ggaaaaggtt tactggaggt
    10141 tgttcatagt ggttaacaac gcggatctac gcgacaaagt aataagaaaa gtactacccc
    10201 gggtgcagac tcctgctcaa tacttgggcg gtgagctcaa ctccgttgtt aaggacacat
    10261 ctcaggtctg tggacacctt tgcctttgta ccccggatac ctacgccatc gggatgagca
    10321 atcacggctt gcaggtcctg tatgatacga tgaaccggcg gaccagttgg gcgtgcgagc
    10381 gggtgtttgc ccctggacgg gatatggagg cagagttgcg ccactggaac ttgcctctat
    10441 atagccttga gacattcacg cccttgtatc gttttgacgt tatcgggttc tcccttcaat
    10501 acgagcttag ctacaccaat gtcctaacgg tgttagacct aggacggatt cccctgcttg
    10561 ccgaggaaag ggacctaaat gatccgctgg tcattgccgg ggggccgtgc gctcagaatc
    10621 cggaaccgat gagttgtttt atcgatcttt tcgtgatnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnngct gcggaagaag cgtaccgtaa taccgggacg aacgagatcg
    10801 ccttgttatc actttcatcc agcgattatc cgcagtttgc cgaacttatc acacggcttc
    10861 gagaagtgtt cgcacctcgc catgtggcaa tctcggtccc tagcttgcgg gtcaacgagc
    10921 aactgcgggt attgacccaa atgttgacgc cggatcgcca ctcaggttta acacttgccc
    10981 ccgaagtcgc gctgaatgac atgcgggtgc aaatcggcaa gcgaattcgc aacgaggacc
    11041 ttattgaggg ctgtcgagag gcattccgca gcggttttga ccgcgtgaaa ttgtatttct
    11101 tgtgtggctt gccaggagag cgtgaggccg acctggcagg tatcgtggag cttgcccagg
    11161 agatttcagg gctgggcaga tcaattctgg gccgtccggc gactgtggtc gctaatgttt
    11221 cgaactttgt ccccaagcct cataccccct atcagtggca tcccatgcag accagggaat
    11281 actttacgca agcgcaccag tttttgcggc agcggtgtcg gatgcggagt gtccagatca
    11341 aatgtcatcc cgtggaaagt agtttgctcg aggggatcat gacgcgaggg gaccggcgaa
    11401 tggggcaggc gatcctggag gcttggcgcc ggggagcacg ctttgacggt tgggccgagc
    11461 aattcaaccc tcaaatttgg tgggacgtct tcgacgatct tgagctgccc gtcgaagaaa
    11521 cgcttcatcg gccgtggagt cctgaagcgc ggctgccttg ggaccacgtc agtatccgcc
    11581 aggggcgagg ctatttagag cgggagcggg accgtgcctg gcgggatctt gccgcactga
    11641 accgataatc caacctcgcc atgggaggca aggggttcgt tcggcggggc acggggcgaa
    11701 cacgattgtg ccccaccgag taatatggaa agtatgcgga gaagcctacc ggctaccttc
    11761 ggccagagaa gctgtcggga agtggcaagg gtgttaagat gaagaaacgt gctcggaggc
    11821 cgttcacgac aagattgagg ggaataacca cctgccagga ttggcagtgt ttgccaaatg
    11881 tgatcgatca tgggctaccg gttcttggca ctggacattg atggaacgct ggtcagtacc
    11941 gtggatgcaa tctcaccatc ggtacgggag gccattgcgc gggctgccga agccgggctg
    12001 gaggttatct tggcgaccgg gcggaggtat agccgggccc tgcccttttc caaaatcctt
    12061 cagattgaga cccccctcgt cacttcgagc ggtgccctga ccaagattcc caaaacacat
    12121 gaaaccttat tcagggcccg attttcggac gaggttctcc ggaagacact catggttctt
    12181 cgggaattgg ggtttcctgc ggccatcctg ggagacacat tcagccacgg ttttgattac
    12241 tacattgagc ggccaaacgg ttatcgcaat ccctattttg aagattacct tgagcaaaac
    12301 tcaggatcag aaaggatttg ggatgacttt ttggcagagc ttccgccgga gattttcgcc
    12361 acgttcaccg ttggtagcga ggccgagatg aaatgggtcg aatgccaact ccacgcggcc
    12421 ctgccgcacc aattgacgac gaacgtcctg cgaagcccgc gatatcgggg gttcttctgt
    12481 gagatcatgc cggcaggaac cacgaaatgg tcagcgattt gccagttggc cgctcaaaga
    12541 ggtgtcacgc cacgagagat ctgcgcggtc ggggatgacg tgaacgacat ttccatgatt
    12601 cgtgaagccg gctttggggt tgccatggga aatgcgcccg acgaggtcaa aaaggcggca
    12661 cactgggttg cgccacccca ggaggaggac ggtctagttg cagtgattga acgactcttg
    12721 gaggaatcat cctgatcgaa tatgtcgggc aaatcagccg cctgagttgt ggagactgaa
    12781 gatgagcgaa acccacgaaa ccattccaga ggagacggga gatcgtgaca taacgttttc
    12841 ggactttcaa cgcctaatcc gtaaaatgta ttttgagaag gacgtggccc gcggtgtcga
    12901 aggtaccttc atgtggctag tcgctgaact gggtgaattg gccgaggccc tgcggagcgg
    12961 gactgccgag gaaaaggctg aggaattcgc cgacgttgtc gcttggttaa cgacgattgc
    13021 caacgtatca aatgtcgacc tcaccgcagc gattcggaag aaatatggtc agggttgccc
    13081 gggatgtggg cggttcgtgt gtgtatgtcc tgacgcgggt aagccctagg tgactgtggc
    13141 tgagatgaga tggaaggatg tgtcaacgag gtgttttggg attgaaaatg tgagcgacgc
    13201 ggatcgatgg attactggcg tgaacaaaca gcgaccgtac aaacggcatt ttccaagtcc
    13261 atgaaacaag ccctcatcct ctttttagag cttctcgtct ggttctcctc gatcgccgcg
    13321 gtgtgtgctg atggagaaac caccgccccg gagatcgtca atttgcgtgt gggcgtcgaa
    13381 ggggtctaca aactcggcct gtggacgcct gtgtgggttg agctccgcgg gggaaccggg
    13441 gaggcctccg gcacgattgt tgtaactgtg ccggacgacg aaggtgggcc atgctcttac
    13501 gttatggaga ccgttaccct gcctgctcag gcaacgcgag aggtcgaaat gctggtccag
    13561 attgggcgac cgcgggccca ggctacagtg cgatgggaag ccgagggtgg accttcggtc
    13621 gaaaaggtct ttcaggccgg caccgcatcc tttccaacgg caatagcggc aaggactccg
    13681 gtatttcttt gggtaggccc atcccaacca ccccaggccc tcatgggaag acgcaacgag
    13741 aaaggacatt gggtttgggt caagaacccc gaacggctgc cgacgcactt cctcggttac
    13801 cagtcggtcc gtaccgtcgt tttggccatc ggtgatgaca aggagtttat tggcagcttg
    13861 cagcgatccg gcaagcaaat gcttgccctt caggaatggg ttcgtgaggg cggcaaagtc
    13921 gtggctagct ttggcccgac tgctaagacg gtcgtcagtg acggtggcgt cctttcgtgg
    13981 gtcgcgcccg gtacgtggca gaatgccatt cccctgagaa aatgtgcagg catcgaaacg
    14041 tatgtggata gtcctcggcc gattgtagtg cgccgcagtg atccgccgct tcaggctctt
    14101 cagtttacga attttgaggg gattgtgacg gcaagtgaag ggaatatccc cttggtcatc
    14161 cggcggcagt acgggtttgg aacgattgtg ttttttacta cggatttgac gcaccgcgct
    14221 ttggtcgaat ggactcagtt tgatcgcctg atagaccggc ttgttggcta ctctgtggcc
    14281 caggatgatg tggccggcaa tccaccgcgg acactgatgc attacggtta cacggacgtg
    14341 agcggtcaat tgcggagtgc cttggacgtc ttgcccacca tatcgtttgt tccattctca
    14401 tgggtagcag ttctcgtggt aatctatctg gtgatcattg gtccggtgga ctattggatt
    14461 ctcggatatg gcatcagaaa ctatcgttgg acttggatca cttttcccgc aatcatcatc
    14521 ctggctgctg gtgctggcat tctcctaggg tacagcaaag gcgagaggcc ggtgacgaaa
    14581 gaggttcact ggattgatta tgatcttgcc ggcagcacaa tacgtgggtt aggactgggt
    14641 agcctgtaca gcctgcaccc cgatttttat gacatcagct tcactgcgcc cgattttgga
    14701 acaaagtcgg aggtgggaca actgtggctg gggtggttcg gcatcccagg ccatggatta
    14761 gggggaatgg aaaataccac cggtgtcctc agttcgcttg agccatacac ggcgatgccg
    14821 cgatcgggat tgttgcaggg atttccagtc aaccagtgga gtactcgcag tgtttgggcc
    14881 ctttggtcgg ctaagagcca aacaccaccg cttcagcttg ccgtgttcga ccgggaggga
    14941 gtgcttcggg gtcgcatcca aaacaatctt gatgtcacgt tggaaaattg tttcgtggcg
    15001 tatggcgact gggtgtatga cctggggacc ttagaaccag gccaggcctg ggagagtttt
    15061 ttgggagacg accgccgcga gctacggtct tggcttaacg ggcggcaggt ctttgttgaa
    15121 ggggaagggg tccgtgccca gacgcggcag cgagtgcagc cctacgaaac agggtcccgc
    15181 gatgttggat acatcctgcg catgatggcc ttctatcagg cggcaggtgg gctcggctat
    15241 gtcgagctgt cgcacgaata ttggccgttt tttgatggca gcgagtggtt ggccaacggt
    15301 cgggccgtga tgattgcgcg ggtccgcacg gagcggcagg cttcacccct ttttggcgta
    15361 acggccagga accaaacgtt cgaaagcgtg actccggttg cgcaggagtg cttttgtcgg
    15421 ttcgtgttgc ccgtaccaaa gggtccgcct cttgcggaat aaccggagag agtaagcaaa
    15481 aaacacattg ttttcaagat gagcccagtt attgagctac ttttatgatc acaaaaaaga
    15541 ctgtcgataa acaagtcgga gtgttacctc aaggtcagac cgattccgat cgcaaatcgt
    15601 cgcgggcggt tgccgtgaag acggtcggtc tgacgaagct gtatggtcaa atccgggccg
    15661 tggatcaatt gaccctggaa ttggaggagg gagatctctt cgggtttatt ggtccgaatg
    15721 gttccggaaa gacaacgacc atgcgaatct tggcgacact ccttcaacct agttggggtg
    15781 aggcctacat ttgtgaccat tcaatctaca cccatcctaa agaaatccgc cgcctcattg
    15841 ggtatatgcc ggatttcttt ggcgtctatg acgacatgaa agtcatcgag tacttggagt
    15901 tcttcgcctc tgcgtatcga atcaatgggc ccaagaggcg caaagtctgt gaggaaatgc
    15961 tggaattggt cgaattgggt tacaagcgcg atgcgtttgt gaccagcctc tctcggggaa
    16021 tgacgcagcg cttggggctg gctagagtcc tacttcatga tccccaagtg ctcatcttgg
    16081 atgaacccgc cagtggtcta gacccgcggg caagaatcga aatccgggag ctactcaagc
    16141 ggttacggca gatgggcaaa accatcatgg tatctagtca catcctgccg gagctggcgg
    16201 atatctgcaa caagattggt attatcgagc aaggcgtcct caaagtgtgt ggcgacctga
    16261 aagaggttat gcaacgagtg cgggaacaga aggtcttgtg tgtagaggtc tgggaaaagc
    16321 aagcggaggc ggtgcggatt ctcgagagcc aagatgatgt ccattcagtg gaggttcgcg
    16381 acggcgttat ctacgtaacc ctgaaaagag gcgttaacga ttatcgtgac ctggcaacgg
    16441 cgctggtcca aggtggctgt ccgctaaaaa tgcttagaga ggatgagatc aatctggaga
    16501 cggcgttcat ggcgctcacg cgggggatga cggcttaagc cgacgccagc gtttcagcag
    16561 attacccacg cccgatgccg gacttgagac gatgcagcac gtcgccggcc tgggttgcca
    16621 agaaagtcag gtcattggac acggcgagca gttgcatgcc ccgtgctgcc cagtgctggg
    16681 cttcgtccac cgtgcgggca tggaatcccg ttgggcaatg ccgttgtcgt cccgcttcga
    16741 tgactttttc aactgcggcc tcaaagtcct ctttgcttgc ctgcggtagt cgctgcggcg
    16801 gtttgcagag gcggcatgcg agatcaagag gtccaattaa caaggcatcg catcctggaa
    16861 taccggcgat attcgacgcc tcttcgaccc cagcggggct ttcgatttgc aagacgatca
    16921 taatttgctc attagccgcc gctaggtatt tctccaaggt tgtctgaaaa cgccgatagt
    16981 gattggctgc tgccgcactt cgggagcctt ggggcggata ctgggatgct gcgatgattt
    17041 gccgggcttg ctcagccgat tccaccatcg ggatgaccaa cccgaaggca cctagatcga
    17101 gtgcccgctt gatgtacaca ggatctcccg cagggacccg taccaggggc gttgtcccgg
    17161 catcgatggc ccatcccacc agtcgatcga cctgggacca gtcaagggcc gtatgctcca
    17221 tgtcaacggt aacccagtcg aagccaattt ctgcaaggat tcggacaact cggtcatcgg
    17281 ggatggtaac ccaggttccc caaacggtcc gaccctcccg caaggccaat tttacggcat
    17341 tcttcttcac agctctaaac ctctgttgag atgaatcccg gtaatggtcg ttccaagcaa
    17401 cgagataacg aacctgcatc ccatttcggc gacccaacag gcggaatcgc ctgaaccgga
    17461 atcagtccag tggaccttgt gaataccgat agaataccac gaggatagac ccgaaaggat
    17521 ggcacctggg catcacatcg tgggtatttt tcctgtgggc ccgtttgggt gcgtgtgacc
    17581 acaaagacga aggagtgcgg aatggacctt tttgattggc aatcgagtgg cgagtggatt
    17641 ccggtctgta agctcgatga cctacaaaaa aacaagggaa agcgatttgt cgtcgcagga
    17701 cgcgagattg ctatctttcg caccgagaat ggcgtttttg caacggatga ttattgcccc
    17761 catatgggtg attcgctcag tgccgggcgg atttgggaag ggaccgtcgt ttgtccgcgg
    17821 cacatgtggg cctttcgtct tgccgatggt cggtgcacgg atgtcccaac gttgtctgtt
    17881 ccaacctaca aggttaccgt ccgcgacggc tgggttctgc tcaaaaagcc agtggaataa
    17941 cgccttgaca cttcgtcgag accatcacct gatacccttc accgccaccg gttaacgagc
    18001 ttatcgcgcg aggaagctac ttgacacctt agccaagcgc gatgctctca cccggctgca
    18061 ggacgacggg cactgatgcg gtggtgtgtc tcacgcgatt ggcccaatca ttgacatcct
    18121 gggcgatcaa aggccatgtg ttaaagtgga caggtacgac ccgtttggga cgcaacagtg
    18181 tgactgcccg caaggcatcc tctggaccca ttgtgaagtt atctccgata ggcaggacgg
    18241 ccagggccaa gtcgtgatcg ccgatcaact gcatgtccag gaaaagaccg gtatcacagg
    18301 caaaatagac gttaccatct tgaagaagta ggaggaatcc cacagccacg ccaccgtaac
    18361 ttccgtcagg aagcattgaa gtatgcaccg ccggtgtcat cttgactcgg ccaaagggga
    18421 atgcatgcgt ccccccgaca ttcatggcgt gtgtcgatgt gacccctctt cggtttagcc
    18481 actcgcaaat ttcgtaagtg gcaatcaccg tggccgcgtt ggcttttgcg atcgactctg
    18541 catcggccat atggtcaaag tgaccgtgag agatcaagac atagtctgcc tttacttcat
    18601 cagctttaat cggcgccaca ggcgactcgt ccagaaaggg gtcaaggatg atacgatggc
    18661 cattggtttc gattagccaa caaccgtgtc ctagccagcg tagctgcgtt gccatgttct
    18721 tggctccctt ctctcaggaa acgccgatga cccaacgcca ttgtgcattg gtagtaccgg
    18781 tttttcagtc cgcggtggcc cccctcccct ttgggaatcg gaattgtcct tggctactgc
    18841 tctaagagtt tctgcatgtg tcgcttgtaa gcctcaactc cgggttgacc gtaagggttg
    18901 attccaatta atcggccctc gacaacagtt gccaacatca gaaactgaaa gagttcaccc
    18961 agcgtgcgtt cccgaatttg cggaagaatg atctcggcgg agcgacgcaa atcctcggca
    19021 taggcttggg tcgttcccag tcgggccgcg cgaagaatat ccgaaagcga ctttcccgcg
    19081 agtttattaa gctgatcatc gtcgtagtcc catcggggaa tcgtaacaca agctagacta
    19141 ggtcgctcag cccagagatt tgtaaaaagt ttgtcccgac ggccttcctg atgctgctgt
    19201 ccgcgcgagt gaagatcgcg tgtattgaca actgtcaacg gcgtagcccc ctgcccgttc
    19261 tttcccagac tttctgaaag gagttggtcg taccacagtc ccacggcttc caaccgcttt
    19321 ccccaggtag ataacacacg tatggaagcc ccacggcggc tttccatgag gtggcagacg
    19381 cccacatatc gcaaaatggg attgctcgcc ggatcactgt tggaaaacgc acgattggca
    19441 gcgcatgccc cttcgagaag ctcctcgatg ttcagtccca gaattgcggc gggcacgagc
    19501 cccacggcgc tcaacacgga aaaacgccca ccgacgtctt ccggaaaggc aaacatgtgg
    19561 tcccgaggta caccaaggtt aacacaaagc cgccaaagtc ggctgtcctc cttgcttacg
    19621 gcgagaattc gctgccccac cttcacgcca cgatcctggc agtgcttttt caacgcctcc
    19681 acaaagaccc ggaaagcaac ggcggtctcg agggtcgagc cgctcttact gatgacaaca
    19741 attccccagt ctgcggtcgg ttctgagtgc aaaagctcta gcagccccag gacggcgtcg
    19801 ttatcgacat tgttaccttc gaagtacaaa cgtggtcggt ttccacgctg agaccgggaa
    19861 agctcgttgt ggtacggatg acagcaggcc tcaaagagcg cacgggcacc catgtaggaa
    19921 cccccgatgc cgaggaccac gacccgctca aggccactcg caaaacgctc ggaggtttgt
    19981 cggatgtgct ccagtaccgt gcgattttcg cccagtagcc gttctggaag gtcgatgaat
    20041 cgagagtcca ggggttcttt ttctttcggg accttgtccc ctcgcttctt gagttccacc
    20101 tcgtccagga cttcgtcgcg gcaggccaag agcctgggcc aaagccactc gaggtcctcg
    20161 gtgaggaccc ctgtttcccg ctcgatggcg ggacgttcgg gggggacttc catgccagca
    20221 cattcgtagc gtagggacaa tcctgatatc tcactttgtc tcatattctc cgcctttctt
    20281 tcgtcacaga aaaagcaatc gaacaggacc gctttccttg tcgggaaacc aactgagcaa
    20341 tcgagcttat tcgcggaccg acagacacaa tatcaggaat tttcccccaa gaatgtcgcg
    20401 atccacaagg ggccgcgtcg cgacggattt tagggaatga ggcaaggcct acgcgatttg
    20461 cccttttgtc ggatcttctt tgcttggcac cgtaggaccg gcatgacggg caatctgtcc
    20521 acggaccatg tagatcacgt cttctgcgat gttggtggca tggtcggcaa ctcgctccag
    20581 atttcgagaa atggcaagca cctgaaggag gggacgaacg aggtcaggcg tctcttggat
    20641 cattcgttcc acgtgttccc gaatcattcg cttttgggca tccaccgcct catcttggcg
    20701 acatacgtcg atcgccagat cgagatcgca attcaccagg ctgtccacgc tgttgcggag
    20761 catcacctgg gcttttcccc acatttcact caaatcaaaa ggcaaccgta tgggcggata
    20821 ctggcttagt gtccgggcct tgcgtgcgat attcacggca aggtcgccga tccgttccaa
    20881 gtcgttattg atctttagga cactgacgag aaacctcaga tcggcagcca cgggctggtg
    20941 gagggtcaag atggtcaaac attcctcctc gatgtcgacc tcggctgcgt cgattttgcg
    21001 gtctgaagac tcgacctcgt cggctagttg gggatcgcgt tttaagaccg cctcaatcgc
    21061 cttgtggagc tggccttcta cctccccaca aaggcgaaga agcctttccc gaagcctgtc
    21121 catctcgcgg tgcatgtgaa cggccatgtc gaggtcctt
//
LOCUS       DAOSUT010000069        20820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1667_length_20820_cov_9.373032, whole genome shotgun sequence.
ACCESSION   DAOSUT010000069 DAOSUT010000000
VERSION     DAOSUT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1667_length_20820_cov_9.373032"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLD05_08080"
     CDS             complement(<1..908)
                     /locus_tag="PLD05_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP77436.1"
                     /translation="MATCLVTGGAGFIGSHIVEALVDRGHKVRVLDNLSTGYLRNLDS
                     VRDQIDFIEGDILDAALVEQVIRGVDWVFHQAALASVPRSVERPLETHAACATGTLIL
                     LDAARKTQVQKFIYAGSSSAYGDQPFSAKRECDLPDPISPYGAAKLAGEYYCRAFYAT
                     YGLPTVVIRYFNVFGPRQDPNSPYSAVIPLFITAILKGKRPVVYGDGLQSRDFTYVAN
                     VVHANLLAAEKEGVAGRVFNAAGGRSITLLQLLAALNEVLGTSVEPEFAPPRPGDIRE
                     SMADISAARKFLGYEPQVDFLEGLRRS"
     gene            1206..3827
                     /gene="alaS"
                     /locus_tag="PLD05_08085"
     CDS             1206..3827
                     /gene="alaS"
                     /locus_tag="PLD05_08085"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOP77437.1"
                     /translation="MKADELREKYLTFFESKGCVRRPSDVLVPRWDPSVLFTPAGMNQ
                     FKDHFLGRCKLEFTRATTCQKCLRTGDIENVGRTAYHHTFFEMLGNFSFGDYFKREAI
                     LWAWEFLTDKKWLGLDPEKISATVYLDDDEAANIWIQEVGLSPEKVTRMGEDENFWPA
                     NAPSQGPDGVCGPCSEIYYETPWGKVEIWNLVFTQFNRVGPPPNNLRPLPSKNIDTGM
                     GLERTAAVLQGVESNFHIDIIRPIVEAAADVCHVKYDPASDFGRRLRRIADHIRACTF
                     AIHENVTPSPNKQGYVIRRLIRRAVLDGHRMGVREPFFHKLVPVVAEMMKNPYPELQE
                     TIPRVTAVIKGEEENFFGVLETGLDRIERLFEEMERSGRGVVSGAEAAELYQTYGFPP
                     ELLETLAAERNFAFDWEGYRREMERHGIESGAGQKKEVFYTGPLDSLKKAGLESQFVG
                     YETTELPGAKVIGLIAAGHLLDSATEQDHGQPIAVVLDKTPFYGEMGGQVGDQGELVA
                     PGLRFEVVDTKVESGFTVHYGHLREGQLTLGMQVTAKVNNWRRQGIRRAHTATHLLHY
                     ALQKVLGKHAQQQGSKVDDDILRFDFANPSAVKRDELAEIEAEVNRRILEAAPVQISF
                     MPLSEARKLGAMMLFGEKYPDVVRVVMVGDYSKELCGGTHLDHAGQVGLFKIVSEESV
                     AAGTRRITAVTGWKAYETVRELSDIMSEVSVALNVPPAEVPARLQAMLREIRQLRKQL
                     ASGGRGVGINADQLWNQSEELDGVRLVISEIPNADADAMRQLIDELRRKKTPVAVLLA
                     GKQADAGKVVIVAGFSADLTKRGLDAVKWVRTVAGVVGGGGGGRADLAQAGGKFPDKL
                     PEALALAREEILKMLQR"
     gene            4237..5610
                     /locus_tag="PLD05_08090"
     CDS             4237..5610
                     /locus_tag="PLD05_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP77438.1"
                     /translation="MNLAFRTDTPRANGRARFIFRIGRLLVAMGLFAVVLCQGNGISI
                     KKSVSVMAIQPPAGQQTATGEELPQEIRKPVTINAQNYPSLQAAFDAVSQNGGLLTIP
                     AGDYVITEPLVVHGSEVRIVGRGPATRIINHNTEGKPAIIVRPADRETNPRARLWRVE
                     FAYLRIQGDPKAIDAKSTEPTSGPGLVAEGVNEILLHALSIDHNGGHGIALIDCYEDP
                     RICDCIITYNRGAGIFLKACHDIVVNANQLEENQQALVCIDSFNLCMNGNNLDDHLGE
                     GIIVENTYGSVVSGNMIEECQGPAIVLDRDCYGITLSSNVLADNFGGAIDLRDAWGCT
                     VSANTFTIDPVFAVRIGPNSGRITITGNNFSDSYIGQQTRRTDKANLATGIVLEGTTD
                     IVISGNIFSGLADPAVTARGGCRRLAITGNIIRDVAQSRAEDRKALDLGDATDVVLAN
                     NILPEAQ"
     gene            5954..6421
                     /locus_tag="PLD05_08095"
     CDS             5954..6421
                     /locus_tag="PLD05_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOP77439.1"
                     /translation="MAKIIVIIFILGITELATLLWLGSYAGWLLALGEILVTLLLGLG
                     IIRWVGVFSFTGTPRLPPIDGFLIVAPIDALVLLVAGVLLMLPGLVTDCLGLLLVFLP
                     TRRLLLRMALWYLGRKLFPSVFSQSDEFESPNPEGDIEVSSDEIIDVEFYRIL"
     gene            6453..7601
                     /gene="nadA"
                     /locus_tag="PLD05_08100"
     CDS             6453..7601
                     /gene="nadA"
                     /locus_tag="PLD05_08100"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOP77440.1"
                     /translation="MSTTLERQTAGALPFAAYKGLSQEELTARIQAVREKMGPRLLIL
                     GHHYQRDEVIELSDLQGDSYRLSALAAENKDCRAIIFCGVHFMAETADILANRPERVK
                     ERRGERIPVVLPDMKAGCSMADMAAIDQVENCWNQLGQVIDLSEVIPVTYVNSAASLK
                     AFCGRHGGTVCTSANADVALQWALSRGRRVLFFPDQHLGRNTALRMGIPLEEMPVWDP
                     QAEELGGQTKEAIQKSRVILWKGYCSVHQIFLPSHVDYYREHYPGIRILVHPECMREV
                     VEKADVVGSTGKIIRAVEEAPPGTKWAIGTELHLVNRLKKKHPEQEIYFLAPVVCMCS
                     TMFRIDLPHLCWAAENLAAGTPVNVIRVDDATAADALVALERMLQLKG"
     gene            7798..8436
                     /locus_tag="PLD05_08105"
     CDS             7798..8436
                     /locus_tag="PLD05_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77441.1"
                     /translation="MKTVSQTKSRPTVSVEQRGFTDEYGNWVALKDPWTAAFLAWLLP
                     GLGHWYQGRWAKAILFFVCITGLFVYGCYLGSRRDLGYARVVYASFRPGDMRLYYLCQ
                     VGVGLPALPALVQSARVRQGKAPLWNYFMAPPRLGTDDGDPPTLSRLCYELHSYFDLG
                     SLYTAIAGLLNLLVVFDAFSGPIFPEDSARREEKAEPRNESSDAVPETTAKQ"
     gene            8471..8698
                     /locus_tag="PLD05_08110"
     CDS             8471..8698
                     /locus_tag="PLD05_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77442.1"
                     /translation="MAFSEDISNPLLMTTMATNYLWYFIPLLIAVSLVYAATRQETMP
                     FILRHAVRTGMWIIIFMGIIAVILQVLDAFT"
     gene            complement(8714..9484)
                     /locus_tag="PLD05_08115"
     CDS             complement(8714..9484)
                     /locus_tag="PLD05_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOP77443.1"
                     /translation="MTEPSDVLVVVVTYNERETLPQLVEAVFNSAPQVDILVVDDNSP
                     DGTGKWCEEYARQNPRMHCLQRPAKLGIGSAVAEGLRWAIDQGYRYVITMDADLSHPP
                     EKIPELLAAIEGSNTTGADVVIGSRYVKGGTIQGWPWYRYLMSWGINSFSRWLLRLPV
                     RDCSGNFRCYRASVLAALNWEEFCSTGYSFFEEILFRLRQEGATFQEIPIRFVERREG
                     RSKMRLREALSAIAAIIRLALLGACSRLRGKAKPQHPR"
     gene            complement(9842..>10491)
                     /locus_tag="PLD05_08120"
     CDS             complement(9842..>10491)
                     /locus_tag="PLD05_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOP77444.1"
                     /translation="VTGRRAFAEAYSEKEGRTAVPLEFPPCGAWFIVFRQSSAEHPPR
                     KESNTSRWVTLREIPEPWRVTFDPRWGGPGTVVFEKLESWTDRPGPGIRYYSGTAIYE
                     TEFTCTDQDLSAHGTHLWLDLGLVRELAEVFVNGRSCGVVWAPPFRVDLAGAVRAGKN
                     ELRIEVVNFWPNRIIGDASLPPEKRLTRTNIRKLTPDTALMPSGIFGPVQIGRLE"
     assembly_gap    10492..10591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10592..13005)
                     /locus_tag="PLD05_08125"
     CDS             complement(<10592..13005)
                     /locus_tag="PLD05_08125"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP77445.1"
                     /translation="MRKALYWFCVAVVVFSLSAGVAKADSLDSGWESPPREARLRAYW
                     WWLNGCVTPQAITRDLEEMKAKGFGGALICDADGSSQDGNERAPHGPDFFSPQWRKLF
                     KYTLQEADRLGLEMSLNIQSGWNLGGPVVSADDAGKKLVWSEHRVTGPCEFRSRLPQP
                     AHRDGYYRDAIVVAYPVKTVSVESPEVRVTASSGQPAHPVDDLADGNPETFWASGGGQ
                     PGQGPSPDRPEWVDFTFSSPVKINRVILRPRPMYGPHKCRLLVSDDGREFRTVADLTL
                     PNPRDERVFLVDLTEGKMFRLMIFGAYDRGSLERPRNVQICELRLSGPEGEWPRAPAR
                     RPITNWAEKAGYRPLHFSAPDTTPLLQEAEPIPGEEDVMPDQVIDLTDKLAEDGTLSW
                     QVPEGTWEILRFGYTVSDRAYVSTCSQGWEGYALDVLDAGAFQRYWEAVVEPLLADAG
                     ALAGKTLKYLHTDSWEIEPLNWTPTLPEEFHRRRGYAITPWMPVLAGRIVGDRRASHR
                     FLYDFRKTLGDLAIDNHYRLFRDNAHKHGLEIHPESGGPHAVPIDAQRCLGFNDVPMS
                     EFWAWSWRHRIGDANRFFVKQPASAAHTYGRRLVAAEGFTTIGPHWQETLWDNLKPSF
                     DKACCEGLNLLFWHAFVCSPEEYGLPGLQYFAGTHLNPNVTWWSKSKPFFDYINRCQF
                     MLQQGLFVADVCYYYGDHVPNFAQHKRTDPAKVLPGYDYDVITAEALLTRATVQSGRI
                     VLPDGMSYRILVLPNHGVISLPVLRKVAELARDGATIVGPRPKESPSLSDDEAEHQRL
                     VAELWD"
     assembly_gap    13098..13197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13691..15196)
                     /locus_tag="PLD05_08130"
     CDS             complement(13691..15196)
                     /locus_tag="PLD05_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP77446.1"
                     /translation="MISKPDMFKVGVLIIVGMCTLVVSGVAGAEETSSVSSIDVNGPA
                     LLALFESTLVDVIARTEKSVVAIARVRTSRPGEVFNLEPRLDPFGGLAERSITPQTGD
                     PNFFPTDYGAGVVVDSKGLILTVAQLIGEESEYYVTTCDRRTFRARVVAADPRSDLAV
                     LSVPAEDLQPITLGNAALLKKGQIVIALGNPYAIAKDGQVCASWGIVSNLGRKAPPMP
                     TDRDPTGKPTIHHFGTLIQTDAKLGFSSNGGALVNLKGEMVGLLTTLPVATGYETPAG
                     YAYPVDETFLRVLNHLKRGEEVEYGLLGIQPESLSLAERASGLQGTRIDRVVPGTPAD
                     RVGLRGRDLLVAVNGEPLYDDDGLVLAVGRLPVDSKVTLDVVRDRRRFQVQAVLTKYP
                     VRGRKIVTAPPQKWRGITVDYVSAVSSSPEGFWQFPRPTDAVAVVAVEENSPAWKAGL
                     RPGAFISHIETEAVTTPKVFYQVAAKYKGPVRLRLVPDGSENYQPVLIIEP"
     gene            complement(15302..15691)
                     /locus_tag="PLD05_08135"
     CDS             complement(15302..15691)
                     /locus_tag="PLD05_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77447.1"
                     /translation="MVHRIRLTSAWTVLSDRSGEIVWRRVFHCPTGLETRSQVWLVIE
                     GPRQPQCVRLNGDRLFDAGAPPETPHKSPLRLEIQTRLKPTKNVLEITFPGHGQFSEL
                     DHQMDGQLLQPIPMEVYLEIQDSESKN"
     gene            complement(15692..16750)
                     /locus_tag="PLD05_08140"
     CDS             complement(15692..16750)
                     /locus_tag="PLD05_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOP77448.1"
                     /translation="MVENLLEVHWWHWCLFSVLIVVLLILDLVVFHRREHRPTLRESA
                     ILSAFWIGLALVFNTLVWWWGWQLHGNSEAGVKFLTGFVVEKSLSVDNLFVFAVIFRF
                     FGVDIRYQHRILFWGILGAIIMRGIFIVAGVGLIQLFEWVLLIFGAFLIYTGVHLAFS
                     NSENVHPEKNVVLRFGRRYLPISKVTIFDRFFIREAGRLCATPAFLVLLVVESTDVLF
                     AVDSIPAVIGITRDPFIVFSSNIWAILGLRALYFLLVGVLDRFHYLHYGLAAVLSFIG
                     LKMVAEYGAHHWFEYHERLINPWVSLAVVGGLLGLSLIPTFLGYKARSSDLTSEAEDE
                     SREGQQRELAKVHDSETR"
     gene            complement(16879..17955)
                     /locus_tag="PLD05_08145"
     CDS             complement(16879..17955)
                     /locus_tag="PLD05_08145"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOP77449.1"
                     /translation="MATQAIEGIFTPNMVPLDERGRINEPELRRYLDWLIAKGVHGLY
                     PNGSTSEFVRFTPEERRRIIEITVDQVAGRVPILAGAAEANVQETIKACEYYARLGVR
                     AVAIVCPFYYRVSPDNIYSYFKEIAKHSPVDVTIYNIPWLASPLDVPTIVRIAEECPR
                     VVAIKDSSGDLPQMMRMITAIRARRPDFSFLTGWDAVLFPMLLLGCNGGTNATSGVVP
                     EITRAIFEAVKAGRIDEARRWQYRLLVYNDALFGAGDFPEGFRIGVEARGFRMGVGRQ
                     PQSEASRERLTRLRRQLEELLVEDRLESIPDGAMASSRATSEMTRSGVARVSGATEEN
                     VREQVERVVREVVHVLAERNVAVN"
     gene            18027..20339
                     /locus_tag="PLD05_08150"
     CDS             18027..20339
                     /locus_tag="PLD05_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77450.1"
                     /translation="MKLRNHRSGSKDIRQGTKPSELPTAGMPLSAQMHLILPILGIIA
                     YLTLFFAWPLLSISQVFQRPFRRYELFQLLLTPEELLKSWLGSHGEFSIFDRLPIFCA
                     AAIILAAGIAWGVAISRVMGVKLSLLEIVSIALPVGLNVISTLVLLGGLFGLLRQRWL
                     LAAIVFLPPTLYIIPWQSLLRLVLKPDVIVQRLRPIFPVQRATTVQSLPEKILSWVCI
                     GATLTAIVIILLAASLPPVDFDVREYHLQCPKEFFQQGRITFLPHNVYGNMPLGLEMH
                     ALLGMLLLNDFWYGALAGKVVMATFALIGAAALLHWARSRGASSPWVAPAIFISVPWV
                     ISVSTAGLNDIGLAVYIFTAFCAASKLVSILTDADQLYPALERGADWATITRENQQPE
                     RNPLNKGCPIRRESWAWVIIAGYLAGAAAACKYTGLLFGGFLIGMFLLVTLAHRIPVR
                     SLLLMLGLYGLGIILGAGGWYIKNLVFSGNPVYPLAYEWLGGTSWNPEKAARWARIHS
                     PHDFSVRMLLVDLGRVFFTSEWQSPLITPLAFLGLLRVSKQRLFEKATAVMAIVLFLL
                     WWCLTHRIDRFWLPMLPFLCVLATVGFDWTDRLWWRWFVTGICLIAMICNGLVITSGA
                     AADNRYFAPLAQLRNDPGRIGADHALLNELFARDHAEGKKKKLLAVGEAAVFDLEMPV
                     LYATCFDDQPWEILTKGKTPKEVRETLHRENVGYLYVNWLEIARYRSPGNYGFTEFVQ
                     PEQFRELTRAGVLVPVLMPTRSPNQIYLVP"
     gene            20513..>20820
                     /locus_tag="PLD05_08155"
     CDS             20513..>20820
                     /locus_tag="PLD05_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77451.1"
                     /translation="MVPLDYSKKYLRYPKKTDFRCPENQLPRLSNKWGLGSAACFCIL
                     LTLFLAVGCRTMDFYDRGLEQPAPTQELEPPRELSKVSLPAYRLEPPDVVQIEVPKMV
                     P"
BASE COUNT         4701 a         5827 c         5515 g         4577 t
ORIGIN      
        1 gaacgcctca ggccctcgag aaagtcaacc tgcggctcat agcctaggaa cttccgcgcg
       61 gcactgatgt ccgccatgct ttcgcggata tcaccaggtc gaggcggggc aaattcgggc
      121 tcaactgacg tgcctaacac ctcattgagg gcagccaaga gctgtaacag cgtaatgctc
      181 cgtccccccg ccgcgttgaa aacccgtccg gcaaccccct ctttttcggc ggctagtaaa
      241 ttggcgtgta cgacgttggc aacataggtg aagtcgcggg actgaagtcc atcgccgtac
      301 accaccgggc gctttccttt caaaatcgcc gtgatgaaaa gcggaatgac cgcggagtac
      361 gggctattgg gatcttgacg gggtccaaag acgttgaaat atcggatgac caccgttggt
      421 aaaccataag tggcatagaa tgcccgacaa tagtactcgc cggccagttt tgcggcgcca
      481 tacggcgaaa ttgggtcagg caggtcgcat tcgcgtttgg cactaaaggg ttgatcccca
      541 taggcactgc tggacccggc gtaaatgaac ttttgaacct gggtcttgcg ggccgcatcc
      601 aagagaatga gcgttcccgt ggcacatgcg gcatgggttt ccaaagggcg ctccacacta
      661 cggggcacac ttgctagggc cgcctgatgg aatacccaat ccacgccccg aataacttgt
      721 tccacaagtg ccgcatcgag gatatcgccc tcgatgaagt caatttgatc tctgacactg
      781 tcgagattcc tgagataacc tgtactgagg ttatccaaaa cccggacctt atgccctcga
      841 tctaccaggg cctcaacaat gtgcgatccg ataaaacccg caccaccggt aactaaacag
      901 gtcgccatcg ccgtcgtcct caatttcaag cacatcaaac cgggccaaat gtcccgatgc
      961 attaacgtag catacacctc attcgttgtg cgattccttc gttgtggaag ttttctgtga
     1021 gaaaatggcc gcgaaggcaa ctgaggcaac ggcgctcaat cttttcgacc ggccaaaaag
     1081 ggggtaagat aaggactttg ccactcgaaa tgaagcccat cggcgaaccg cgatggcggt
     1141 ccgccgagtc ttaacgattg tgccaatctt agcactttcc cgaatcaaaa ggatgacgca
     1201 tttttatgaa ggcggatgaa ttaagagaaa aatacttgac attttttgag tccaagggtt
     1261 gtgttcggcg gccgagtgat gtccttgtcc cccggtggga cccgtccgtt ctgttcaccc
     1321 cggccggcat gaaccagttc aaggaccatt tccttggtcg gtgcaagctg gagtttaccc
     1381 gggcgacaac ctgccaaaag tgcctgcgca cgggcgatat cgagaatgtc ggccgaaccg
     1441 cctaccatca cacattcttc gaaatgctgg gcaatttcag ctttggagat tatttcaaac
     1501 gggaggccat cctctgggcc tgggaattcc taacggacaa gaagtggctt ggtctcgacc
     1561 cagagaagat ctcggccact gtgtaccttg acgatgacga ggcggccaac atttggattc
     1621 aggaagtggg cctgtcacct gaaaaggtca cgcggatggg cgaagatgaa aacttttggc
     1681 cggccaacgc ccccagccag gggcccgacg gcgtttgcgg accgtgcagc gagatttact
     1741 acgaaacccc gtggggaaag gtcgagattt ggaatctggt gttcacccaa ttcaatcggg
     1801 tgggaccacc ccccaataat ctcaggccgc tgcccagcaa gaatatcgac accggcatgg
     1861 ggctagaacg tacggcagcc gtcttgcaag gggtcgagtc gaatttccac attgacatta
     1921 ttcgtccgat cgtcgaggca gccgccgacg tatgccacgt caagtatgat ccggccagcg
     1981 attttggacg gcggttgcga cggattgctg atcacattcg cgcctgtacg tttgccattc
     2041 atgagaacgt gaccccttcg cccaataaac agggttacgt gattcgccgt ttgattcggc
     2101 gtgccgtgct ggatggtcac cgcatgggtg tccgcgagcc gttcttccac aagctcgtgc
     2161 ctgtcgtggc cgaaatgatg aagaatccct atccggaatt gcaggagacc attccgcggg
     2221 tcaccgcagt catcaaaggg gaggaagaga atttctttgg cgttctggaa acgggcctgg
     2281 accgaattga acgtcttttc gaggagatgg aacgcagtgg tcggggggtg gtctcggggg
     2341 ccgaggctgc cgagttgtat caaacctacg gatttccgcc ggaacttttg gagacgcttg
     2401 ccgccgagag aaactttgcc ttcgactggg aaggctatcg acgcgagatg gaacgccacg
     2461 gcattgaatc cggggccggt cagaagaaag aggtctttta caccggtccg cttgattccc
     2521 taaaaaaggc gggcctcgaa agccagtttg tcggttatga gacgaccgaa ctacccggtg
     2581 ccaaggtgat cggcctgatc gctgctggtc atctcctcga ttccgcaact gagcaggatc
     2641 acggtcaacc gatcgccgtg gtcttggaca agacgccctt ttacggcgaa atgggcggtc
     2701 aagtcggtga ccagggtgag cttgtcgctc caggactccg ctttgaagtc gttgatacga
     2761 aagtggaaag tggcttcacg gtccactatg gacacctccg cgaaggtcaa ctcaccttgg
     2821 gaatgcaggt gaccgccaag gtcaacaact ggcggcgaca agggattcgt cgggcccata
     2881 cagcgacgca cttgctgcat tatgcccttc aaaaggtcct gggcaaacat gcccaacagc
     2941 aaggctccaa ggtcgatgat gacatcttgc ggttcgactt tgcgaacccg tcggccgtga
     3001 agcgcgacga actggcggag atcgaggctg aggtcaatcg tcgcatcttg gaggcagccc
     3061 ctgttcaaat cagctttatg ccgcttagtg aagcccgaaa actcggggca atgatgctct
     3121 tcggcgagaa gtatcccgac gtggtacgag ttgtgatggt tggcgattac agtaaggagc
     3181 tttgcggcgg gacacacctc gatcacgcag gccaagttgg attgtttaag attgtcagcg
     3241 aagagagcgt tgccgcgggg acacgtcgca tcacggccgt gacgggttgg aaggcttatg
     3301 agactgtccg cgaactttcc gacatcatgt ccgaggtgtc ggttgccctc aatgtgccac
     3361 cggcagaagt ccctgcccgg cttcaggcga tgctgcggga aatccgacaa ctccgcaaac
     3421 aattggcttc cggcgggcga ggcgtgggaa tcaacgccga tcaactctgg aatcaatcgg
     3481 aagagcttga cggtgtccgc cttgtcatca gtgaaatccc caatgcagat gccgacgcga
     3541 tgcgtcagct cattgatgaa ttacgtcgca agaagacgcc ggtggccgtc ctcctggcag
     3601 gaaaacaggc tgatgccggg aaagttgtca tcgttgcggg attctccgct gatctgacca
     3661 aacgtggcct agacgccgtg aaatgggttc gcacggtggc gggtgtcgtg ggcggcggcg
     3721 gtggtggccg agctgatttg gcccaagctg gcggcaaatt ccccgataaa cttcccgagg
     3781 ccctggccct tgcccgggaa gaaatcctca agatgctcca gcggtagttc ctcggtcgcg
     3841 cacgtaattg cgattgcgat agtagcacag ccgaagtgcg tgtgttgcgt ccggacgaaa
     3901 aagcacttgc gcgcgggcgc ttttttgtac ggccccgata gttcgttgtc ctccgttacg
     3961 aaatcattgg tgggacgcgt tccggtttaa tccgtatccg aaccgaggca cttcggcccg
     4021 tccacctcag tgtgttctct tcgtcgcggc gcctgtctcg ccggataggg gggcgagcgg
     4081 ctccctatcc ctttctggtg gtgttcggcc aggcagtgtt ttgattacaa tatcgggcga
     4141 ggttggcgac tgctggaagg tgcccctgct gacgtttggg tggaagatgc tttttttatt
     4201 cccctggtaa acccttagcc aagaggagtg attgccatga atctcgcctt tcgaaccgac
     4261 acaccgcgtg ccaatggccg cgctaggttt atttttcgca tcggccggct ccttgtcgcg
     4321 atgggacttt ttgccgtcgt gctctgtcag ggtaatggca tatcgattaa gaaatccgtc
     4381 agcgtgatgg ccatccaacc acctgccggc caacaaacgg ccacaggaga ggaattaccc
     4441 caggaaatcc gcaaacccgt tacgatcaat gctcagaact accccagtct tcaggcggcc
     4501 ttcgacgccg tttcgcaaaa tggcgggctt ctcacaatcc cagcgggtga ctacgtcata
     4561 acagaaccgc ttgtcgtcca tggttcggag gtccgcatcg ttggcagggg ccctgccacc
     4621 cgaatcatta accacaacac agaaggcaag cctgcaatca tcgttcgacc cgccgatcgt
     4681 gagaccaatc cgcgggcaag gctttggcgg gttgagtttg cgtatttgcg aatccaaggc
     4741 gatcctaagg cgatcgacgc aaaaagtacc gaaccgacca gtgggccagg gctggttgcc
     4801 gaaggggtca atgaaatcct ccttcacgca ctgagcatcg atcacaacgg cggtcatggg
     4861 attgccttga tcgattgcta cgaagaccct aggatctgcg actgcattat cacgtacaac
     4921 agaggtgcgg gcatctttct taaggcgtgt cacgacatcg ttgtcaatgc caatcaattg
     4981 gaggaaaacc agcaggccct tgtatgtatc gatagcttca acctttgtat gaacggcaac
     5041 aacctcgacg accatttggg cgaggggatt atcgtcgaaa acacgtatgg ttcggtcgtc
     5101 tcgggaaata tgatcgagga atgccaggga ccggccattg tcttggaccg ggattgctac
     5161 ggtattacgt tgagttccaa cgtccttgca gataatttcg gaggggcgat tgatctccgc
     5221 gacgcatggg gctgcacggt gtccgcaaac acgttcacca ttgatccggt ttttgcggtc
     5281 agaattggtc ccaacagtgg gcggattacg attacgggaa acaatttctc cgacagctat
     5341 atcggtcagc aaactcgccg cacagataag gcaaacctgg ccaccggcat tgtccttgag
     5401 ggaacaacgg atatcgtgat ttcgggcaat attttttcgg gcttggccga cccggcggtc
     5461 accgcccgcg gtggttgccg tcgtctggcg atcacgggaa atattattcg cgacgtcgct
     5521 cagtcgcggg cggaggatcg caaggccctt gatttgggtg acgccacgga tgtcgttctc
     5581 gctaataaca tcttaccgga agcgcaatag gctcggcgaa atagcgaagg gaggctttgc
     5641 ccctaacggg cttggaggta tcgtcaacgt ctgcgatctt ctgccctgtg atgtgtaagg
     5701 gcctgtgggg acaggagatt cgctggtccg cgtgtctcct gtgattgcgc ctggttcaac
     5761 ttggtggtat tcacctgtgt gcagaatttg tatactagcc gccggtacca attagcgggt
     5821 gctcgtggcc cggccttatt caaagcggta gaataaatca aggcagctac cgggcggtga
     5881 tgaaacgttc cgccacaagt ggggccttgc tattggccct tggcaagata ggctgaaaag
     5941 gaatattcgg gtgatggcga agatcattgt tattatcttc attctcggta ttaccgaatt
     6001 ggccaccctt ctttggctag gaagttacgc cggatggtta cttgcactcg gggaaatcct
     6061 ggtgacgctc ctcctgggac tgggtatcat ccgttgggtg ggcgtttttt cctttactgg
     6121 gactcctcga ctgcccccaa tagatgggtt tctaattgtc gcgccgattg atgctttggt
     6181 tttgctagtg gctggtgtat tgctcatgtt gccaggctta gtcactgact gcctgggcct
     6241 tctactagtt tttctaccta cgcggcggtt actactgcgc atggcgcttt ggtatttagg
     6301 aagaaaactg tttcctagcg tgttctctca gtccgacgag tttgagtctc ctaatcccga
     6361 gggggacatc gaggtgagct ccgacgaaat tattgacgtt gaattctacc gtattttgta
     6421 gtcatcgagt ttttggaaag cttggagagt tcatgagcac aacactggaa cgccaaacag
     6481 cgggagctct tccgttcgct gcctataaag gtctttccca ggaggaatta acggcccgta
     6541 ttcaggccgt ccgtgaaaaa atgggcccgc gacttttgat cctggggcat cattatcagc
     6601 gggatgaggt cattgaacta tctgacctgc agggagacag ttaccgcttg agcgccctgg
     6661 cagccgaaaa caaggactgc cgggcgatca tcttctgtgg cgtccatttt atggcagaaa
     6721 ccgccgacat tctggccaat cgtccggaac gcgtcaaaga gcggcgtggc gaaaggattc
     6781 ccgtcgtcct ccccgacatg aaggccggtt gctcaatggc cgacatggcc gcgatcgacc
     6841 aagtcgaaaa ctgctggaac caactggggc aggtcatcga cctaagcgag gtgatcccgg
     6901 tgacctacgt caattctgcc gcgtcgctta aggcattttg tgggcgccat ggggggacgg
     6961 tgtgtacctc ggccaacgcc gacgtagcct tacaatgggc gctatcgcgt ggccgacggg
     7021 tgctgttttt cccggatcag catttaggcc gcaacacagc cctgaggatg ggcatcccct
     7081 tggaagaaat gcccgtatgg gaccctcaag ccgaggaact gggcggacaa acgaaagagg
     7141 ccatccaaaa gagtcgtgtc atcctttgga aggggtattg tagcgtacac caaatctttt
     7201 tgccctcgca tgttgactat tatcgagagc actaccctgg catccgtatc ctggtccatc
     7261 ccgaatgtat gcgggaggtc gtggaaaagg cggacgtagt gggatcgacg ggaaagatta
     7321 ttcgtgccgt ggaggaggcc ccaccgggca caaaatgggc catcgggaca gagctccacc
     7381 tggtcaatcg cctgaagaag aagcacccgg aacaagaaat ctatttcctc gccccggtgg
     7441 tgtgcatgtg ctcaaccatg ttccggatcg acctgcccca cctttgttgg gccgcagaga
     7501 atttggcagc cgggacgccg gtcaacgtga ttcgtgtgga tgatgcaacg gctgctgacg
     7561 ctctggttgc cctggaacga atgttgcaac tcaagggcta gatgcgcaaa aggtggtatt
     7621 gccttgtatc ttttgcggta tgttctgccc ttggtcataa cgttaggaaa cttgacaact
     7681 tgggttgcaa aaaagcaccc tgtcgggtag tctcaaaggt taaggatcgc tggaccttct
     7741 tccctttctt cctgcccaat gcgaattctc ctggtttagt ggatagcaag aacaatcatg
     7801 aaaacagtgt cgcagacaaa atcgcggcca accgtttcgg ttgagcagcg cggttttacg
     7861 gatgaatatg gaaactgggt ggctttgaaa gacccttgga ccgctgcatt cttggcctgg
     7921 ctgctccctg gtctggggca ttggtaccaa gggcggtggg ccaaggccat cctctttttt
     7981 gtttgtatta cgggcttatt tgtttacggg tgttacttag ggagtcgccg cgacttgggc
     8041 tatgcccgtg ttgtctatgc ctcctttcgt cctggtgaca tgcggcttta ttacttgtgt
     8101 caagttgggg tgggacttcc ggcacttccg gcactcgttc aatcggcacg tgtccggcag
     8161 ggaaaggccc cgttatggaa ttactttatg gcgccaccgc gcttgggcac ggatgatggc
     8221 gatccgccga ccctttctcg cttatgttat gagcttcact cctatttcga tttagggagc
     8281 ctttacacgg ccattgccgg cttgctcaac ctgttggtcg tttttgacgc cttctccggg
     8341 ccgatcttcc cggaagattc cgcccgtcgt gaggaaaagg ccgaaccccg gaatgaatca
     8401 tccgatgccg tgccggagac caccgccaag caatagtttt tttgatcaac tgtttaacgg
     8461 agcgaaatcg ttggcattct cggaggatat ttccaatcca ctccttatga caactatggc
     8521 aacgaactat ctttggtact tcattccgct cctgatcgca gtcagtcttg tttacgcagc
     8581 aacgcggcaa gagacgatgc ccttcatcct tcgccatgcc gtgcgaacag ggatgtggat
     8641 tatcatcttc atgggcatta tcgctgtcat cctacaggtc cttgacgcgt ttacctgaac
     8701 gatgggccga gcgttacctc gggtgctgag gtttggcctt tcctcgaagg cgggaacagg
     8761 caccaagaag ggccaaccga atgatggccg caatcgccga gagggcctcc cgtaatcgca
     8821 ttttcgaccg gccctcgcgt cgctcgacaa agcgaattgg gatctcctgg aaagtggccc
     8881 cttcttgtct taaacggaat agaatctcct caaaaaacga gtaccctgtg gagcaaaact
     8941 cctcccagtt caaggccgct aaaaccgacg cccggtaaca tcgaaaattg ccgctacaat
     9001 cgcgaactgg caaccgaagg agccaccggg aaaagctgtt gatgccccaa ctcatgagat
     9061 agcgatacca cggccaacct tgaatggttc cgcctttcac atagcgcgaa ccaataacga
     9121 catccgcccc tgtcgtattt gacccttcga ttgcggcaag caactcggga atcttctccg
     9181 gaggatggct caaatcggca tccatcgtaa taacgtaccg atatccctga tcaatcgccc
     9241 agcgaaggcc ctcggcaacc gcgctcccaa tgccaagttt tgccggccgt tgaaggcaat
     9301 gcatacgcgg gttctggcgg gcatattctt cacaccactt ccctgtgcca tccggcgagt
     9361 tgtcgtccac caccaggatg tcaacctggg gggctgagtt gaagacggcc tccacaagct
     9421 gtggaagggt ctccctttcg ttgtaagtta caacaacaac aagtacgtca gaaggttcgg
     9481 tcatcggctt ccgcggtctg cctcatgcca tgtgttcagg tcaattcctt tcacgttcat
     9541 tctctgaccg acttaccgag ttgacaagcc gctggcacgt ctactgagag agcacttaga
     9601 agcaatctct cccctcgccc attcggtggg agatggatcg ggaggagggt ggctctggtt
     9661 gctaccggtt ttttcaatcg accccggtga tcgcttgccc tcaccccaac cctctcccgg
     9721 agggagaggt ggactttttg ttgagataga ctcttaaagg cttccgcggc gtagatgcca
     9781 tgatgcgacc gccagcgtaa tccgcgcttg gggaaacgtg ccccagtgtc gcagttatca
     9841 cttattccag tcgcccaatc tgtacggggc cgaaaattcc cgaaggcatg agcgcagtgt
     9901 cgggcgtcaa cttacgaata ttggtccgtg ttaatcgctt ttctggcggc aaggaggcat
     9961 caccaatgat gcggtttggc cagaagttga caacctcaat gcggagctcg tttttccccg
    10021 cgcggaccgc cccggcaaga tcgacgcgga aaggcggtgc ccacaccacg ccgcacgaac
    10081 gcccgttcac aaatacttct gccaactccc gtaccaatcc caagtccaac cacagatgcg
    10141 tgccatgtgc ggaaagatcc tgatctgtac acgtgaattc tgtctcgtaa atggccgttc
    10201 cagagtagta tcgaatgccc ggtcccgggc ggtcggtcca gctttcaagc ttttcgaaga
    10261 caaccgttcc aggaccaccc catcggggat caaatgtgac gcgccaaggt tcggggattt
    10321 ctcgcaacgt gacccaccgt gaggtgtttg attcctttcg gggtggatgc tctgcagacg
    10381 attgccgaaa tacgataaac cacgccccac acggtgggaa ctccaagggc acggccgtcc
    10441 gtccttcttt ttctgaatat gcttcggcaa aagcccgccg acccgtcacg gnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgtcccac aactcggcaa ctaaccgctg
    10621 atgttccgct tcgtcgtcgc tcaaactggg ggattctttg ggccgcgggc ccacaatggt
    10681 cgcaccgtct ctggctaact ccgccacctt gcggagaacg ggcagcgaaa tcactccgtg
    10741 gttaggcaaa acgaggatac gataactcat accgtccggg agcacgatgc ggccggattg
    10801 aactgtggcc cgcgtcaaca acgcctccgc cgtgataacg tcgtagtcat aaccgggtaa
    10861 aaccttggcg gggtccgttc gcttgtgttg ggcaaagttg ggaacgtgat cgccgtaata
    10921 ataacaaaca tcggcgacaa aaaggccctg ttgaagcata aactgacagc ggttgatata
    10981 gtcgaaaaac ggcttggatt tcgaccacca tgtcacattg ggattgaggt gcgtcccagc
    11041 aaaatactgg aggcctggca ggccgtactc ctcgggcgaa cacacaaagg cgtgccagaa
    11101 cagcaggtta aggccttcgc agcacgcctt gtcaaaggaa ggtttgagat tgtcccacag
    11161 ggtctcctgc cagtgtgggc caatggtcgt aaacccctcg gcggccacca agcggcgacc
    11221 atatgtgtgc gccgccgagg ccggttgctt aacgaaaaaa cgatttgcat cgccaatacg
    11281 atgacgccac gaccacgccc aaaactcgga cattggaaca tcattgaatc ccaaacaccg
    11341 ctgggcatcg atgggaaccg catggggacc gcccgattct gggtgaattt ccaacccgtg
    11401 cttatgggca ttgtcccgga acaaccgata gtggttgtca atagccaagt cccccagtgt
    11461 cttgcggaag tcgtaaagaa accgatgact tgcacggcga tcgccgacaa tccgtccggc
    11521 caacaccggc atccatgggg tgatcgcata accccgacgc cggtgaaatt cctcaggaag
    11581 cgtgggtgtc cagtttaggg gttcaatttc ccaactgtca gtgtgaaggt acttcagcgt
    11641 cttgcctgcc agcgcccctg cgtcagccag aagaggttcg acgactgcct cccagtaacg
    11701 ttgaaatgcc ccagcatcaa ggacgtccaa agcatagcct tcccatcctt gactacacgt
    11761 agaaacatag gcacgatcgc tgacggtgta accgaaccgt aaaatttccc acgtcccttc
    11821 cggcacctgc caggaaagcg tcccgtcttc cgccagcttg tcggtgagat caatgacctg
    11881 atctggcata acgtcttctt cacctgggat tggttcggcc tcttgcaaaa ggggtgttgt
    11941 atctggcgcc gaaaaatgta ggggtcggta acccgccttc tccgcccagt tagtgatcgg
    12001 acgacgggcc ggtgcccgcg gccattcccc ttccggacca cttagtcgca attcgcaaat
    12061 ctgcacgttt cgcggacgct caagcgatcc ccgatcgtat gccccgaaga tcatgaggcg
    12121 aaacatcttg ccttccgtca aatccaccaa aaagactctt tcgtcccggg gatttggcag
    12181 cgtcaggtcg gcaacggtac gaaactcacg accatcatcc gagacgagca aacgacactt
    12241 atgtggccca tacattgggc gagggcgcaa gatcacacgg ttgattttga caggcgacga
    12301 gaaggtaaaa tcaacccact cgggccgatc cggcgatggt ccctgtcccg gctggccccc
    12361 tccggaagcc cagaatgttt ccgggttccc gtccgcaaga tcatcgactg gatgggccgg
    12421 ttgtcccgac gatgcggtca cccgaacctc tggagattcg acggacacgg ttttcacagg
    12481 atacgccaca acaatcgcat cgcggtaata gccgtcacgg tgtgccggtt ggggaaggcg
    12541 cgaacgaaac tcgcaaggtc cagtcacacg atgctctgac catactagtt ttttgcccgc
    12601 gtcatcggcg gagacaacag gtcctccaag attccagcca ctctggatat tgaggctcat
    12661 ttccagcccc aagcgatcgg cctcctgcaa ggtatatttg aacaatttcc tccactgcgg
    12721 tgagaagaag tcgggaccgt ggggtgcgcg ttcattgcca tcctggctcg atccatccgc
    12781 gtcgcaaatg agtgccccgc caaagccctt ggccttcatt tcctcaaggt cccgcgtaat
    12841 cgcctgaggg gtcacacagc cgttcagcca ccaccaataa gcgcggaggc gcgcctcgcg
    12901 gggtggcgac tcccagcctg aatcaagtga atccgccttg gcgacaccgg ctgacaacga
    12961 aaaaacgacc acagccacac aaaaccaata caacgccttt ctcatggtag cgcaatcctg
    13021 caaaagactt cgaaagattt ctcggtactg tgctgtatta tggaacacga tccgcccctt
    13081 tgcaatttga cgaagacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
    13201 tactgtgctg tattatggaa cacgatccgc ccctttgcaa tttgacgaag acgttcgccg
    13261 agcacactgc ggtaggagac accaagcaca ctgcgaaagg agacgtcatt tcagggtaat
    13321 ttgcgaacct actttcccaa cgggtctcga atgcagaaat tgagatacct cgctgttcac
    13381 tcgggattat caagaaggat agaaaaggtc cgcctttgct ggtcagaggc gaataaagca
    13441 cgacggccgt cccgctaaca cccttcgcta aagacccctt agacaaccca tgacgaaagc
    13501 ctcggtcggc taagagccta tcccgtaacc ccccctcagt cgttcggcgg gagaggcgcc
    13561 cggggttgag ggtggctcga atcgctactg gttttctcga tcgacttcga cgatcactag
    13621 ccctcacccc agccctctcc cagagggaga gggaactttc ccagagggag ggggaacttt
    13681 gggataggct ttaaggctcg atgatcaaga cgggttgata gttctcggaa ccatcgggga
    13741 caagacgcaa acgaaccggt cccttatact tagcagccac ctgataaaag accttgggcg
    13801 tggtcacggc ttctgtctcg atgtgcgaaa taaacgcgcc cggacgaaga cctgctttcc
    13861 atgcgggact attctcctcc acggccacga ctgccactgc atccgtggga cgcggaaact
    13921 gccagaaacc ttcaggactt gacgagaccg ccgagacata atccaccgta atgcctcgcc
    13981 atttttgggg cggcgctgtc acaatctttc ggccacgaac tggatacttc gttaagacag
    14041 cctgcacttg aaagcgacgc cggtcacgga cgacatccag tgtgaccttt gagtcaacgg
    14101 gcaacctccc caccgccagt accaacccat cgtcatcgta gagaggctca ccgttgaccg
    14161 ccacgaggag atcccgaccg cgcaggccga cgcggtccgc aggtgttccc ggtaccaccc
    14221 gatcaatccg cgtcccttga agaccgcttg ccctctcagc cagcgacaaa ctttctggct
    14281 gaatgccgag caaaccgtat tcaacctctt ccccgcgttt caggtggttt agaactcgga
    14341 ggaaggtctc atccaccgga taggcataac ccgcgggggt ttcgtaacct gtggcgacag
    14401 gaagcgtggt taacagtccc accatttccc ccttcaaatt cactaacgca ccaccgttac
    14461 tggagaagcc caacttggcg tccgtttgaa tcagggtccc aaaatgatgg atggtaggct
    14521 tgccggtggg atcccgatct gtgggcattg gcggcgcctt gcgaccgagg ttcgacacaa
    14581 tcccccaact tgcgcacacc tgaccgtcct ttgcaatggc ataaggattg cctaatgcga
    14641 tgacgatttg gccttttttc aagagggcag cattccccag cgtaatcggc tgcagatcct
    14701 ctgccggaac ggaaagtacg gccagatcac tgcgcggatc ggccgccaca acgcgcgccc
    14761 gaaacgtccg ccggtcgcag gtcgtcacgt agtattcact ttcctcacca atgagttgtg
    14821 ccacagtaag gatcaaacct ttggagtcca caaccacgcc cgccccgtag tccgtgggaa
    14881 agaaatttgg gtcgccggtt tgcggtgtga tactccgttc ggccaggccc ccaaatggat
    14941 ccaatcgcgg ttccaaattg aaaacctccc ctggccggct ggtgcgaacc cgggcaatcg
    15001 ccaccaccga tttttctgtc cgcgcaatga catctacgag cgttgattcg aacagggcga
    15061 gcaaggcagg gccgtttacg tcaatgctgg acactgacga agtctcttcg gcaccggcaa
    15121 caccggaaac gacaagggtg cacatgccca cgatgataag cacacccacc ttaaacatgt
    15181 caggttttga aatcacggca cacctcgcct cgcgattgag ccaatttccc cttcacgcca
    15241 acatcaaaac cagtgttttt aatgacgatt cggatatgcc ccaagataaa caaaaagttc
    15301 cttagttctt tgactcgcta tcttgtattt caagataaac ctccatcggg atgggctgca
    15361 acaactgccc atccatttgg tggtccaatt ccgaaaattg cccatgacct ggaaaagtga
    15421 tttccaaaac atttttggtc ggcttcaaac gggtctgaat ctcaagcctc agcggcgact
    15481 tatgcggagt ttcaggaggg gccccagcat caaacagtcg atcaccatta agtcgtacac
    15541 actgcggttg ccgaggtccc tcaatcacaa gccacacttg cgagcgggtt tccaggcctg
    15601 tagggcaatg aaacacccgc cgccagacga tttcgcccga acgatcgctc aagactgtcc
    15661 acgctgatgt caaccgaatc cgatggacca actaacgagt ctccgaatcg tgtactttgg
    15721 ccaattcgcg ttgctgtccc tctcgacttt catcttcggc ttcggatgtt aagtcggagg
    15781 atcgggcttt ataacccagg aatgtgggga tcaaagacaa acccaacaat cccccaacaa
    15841 ccgcaaggga tacccaaggg ttgatcaggc gttcatggta ttcaaaccag tgatgcgcgc
    15901 cgtactcggc caccatcttc aggccgatga acgaaagcac cgctgccagg ccgtaatgaa
    15961 ggtaatgaaa ccggtccaga acgcccacca ggagaaaata caacgcccgc aagccaagga
    16021 tggcccagat gttcgaggaa aaaacaataa acggatcacg cgtaattccg atgacagcgg
    16081 gaatgctatc gacggcgaac aaaacgtccg tgctttcgac gacaagcagc accagaaaag
    16141 ccggcgtggc gcacagtcgg ccggcctctc gaatgaaaaa acgatcgaaa atagtgactt
    16201 tgctaatcgg cagataacgg cgaccaaatc gtaagacgac attcttttcc ggatggacat
    16261 tctctgaatt ggaaaacgca aggtgaacgc cggtgtatat gaggaatgcc ccaaagatca
    16321 gaagaaccca ctcgaaaagt tgaatcagcc ccacgccggc cacaataaaa atgcctcgca
    16381 taatgatcgc cccgagaatg ccccaaaaga gaatgcgatg ctgatagcgg atatccaccc
    16441 caaaaaaccg aaagatgacg gcaaagacaa aaagattatc aaccgagagg gacttctcta
    16501 cgacgaaacc tgtgagaaac ttcacgccgg cctcagagtt gccatggagc tgccaccccc
    16561 accaccaaac aagtgtattg aacaccagtg cgagcccgat ccaaaacgcg gagaggatcg
    16621 ccgattcccg cagagtaggc ctgtgttccc gccggtggaa aacaaccaga tcgagaatca
    16681 acaagacgac aatcaggact gagaataagc accaatgcca ccaatgaact tccagcagat
    16741 tctcaaccaa gaggcactcc tttctccgac ccaacactcc aaatccgtag ctttggaaat
    16801 ggccaaccac tcggagagtg gggcgcaggc cttcgcgccc tcggatcatg gatcatcaca
    16861 aaagacctac atcatccttc agttgacggc aacattccgt tccgccaaaa cgtgaacaac
    16921 ctcccgcaca accctctcca cttgttcccg aacgttttcc tccgtggccc ctgaaacccg
    16981 ggcaacacct gatcgtgtca tctccgatgt ggcgcgggaa ctcgccatcg cgccgtccgg
    17041 aatcgattcc aaacggtcct ccaccaggag ctcctccaat tgtcgccgca atcgggttag
    17101 ccgctcccga ctcgcctcag actgcggctg acgccccaca cccatgcgaa agccgcgggc
    17161 ttccactccg attcggaatc cttctggaaa atcacccgcc ccaaaaaggg catcgttgta
    17221 cacaagcaat cggtactgcc accgtctcgc ctcgtcgatt cgtcccgcct tcaccgcctc
    17281 gaaaatggcc cgggtaatct ccggtaccac cccactcgtg gcattcgttc cgccattgca
    17341 acccaaaagt aacataggaa acagaacagc atcccaacct gtcaaaaagg agaaatctgg
    17401 acgacgcgcc cgaatggccg tgatcattcg catcatttgc ggaagatcac ccgacgaatc
    17461 cttaatcgcc acaactcgcg gacattcttc ggcaatccta acgatcgtcg gtacatccag
    17521 gggcgaggcc aaccatggga tgttgtaaat cgtcacatcg accggactat gtttggcaat
    17581 ctccttgaag tagctgtaaa tattgtccgg actgacgcga taatagaacg ggcagacaat
    17641 agccaccgcc cgtacaccaa gcctcgcata gtattcgcaa gccttgatcg tttcttgcac
    17701 attggcttcg gcggcacccg ccaagatggg gactcgcccc gccacctgat ccaccgtgat
    17761 ctcaatgatc cggcgacgct cctcaggggt aaaccgaaca aactcactgg tggaaccgtt
    17821 gggataaagc ccatgaacgc ctttcgcaat caaccaatcc aaataacgcc gcaactccgg
    17881 ttcgttgatc cgtccccgct cgtcgagggg aaccatgttc ggtgtaaaaa tgccttcaat
    17941 tgcctgggtt gccatacgac aaggacgcct tatgctggaa acggagggaa atcggtagcc
    18001 aactttgcat gctccggatg gtcaaaatga aactccgcaa tcatcggtcg ggttcaaagg
    18061 acatcaggca ggggactaag ccctcagaat taccgaccgc aggcatgccg ttgtctgcgc
    18121 aaatgcatct gattttgccc atcctaggaa tcatagccta tttaaccctt tttttcgcct
    18181 ggcccctcct cagcatttcg caggtgtttc aaagaccttt ccgacggtac gagctgttcc
    18241 aattgttgct aacgcccgaa gaactcctta agtcatggct ggggagccac ggagaattct
    18301 ctatcttcga cagactcccc attttctgcg ccgcagccat aattctcgcc gccggtatag
    18361 cttggggagt cgctattagc cgggttatgg gagtgaagct tagccttctg gaaatcgtaa
    18421 gtatcgccct accggttgga cttaacgtca tttcaacgct cgtcctgctc gggggccttt
    18481 ttggtttact ccggcagcga tggcttcttg cggcaatcgt gtttcttccc ccgacgctct
    18541 acattatccc gtggcagtcg ttgctgaggc tggttctcaa acccgacgta attgtgcaac
    18601 gcctccgccc tatttttccc gttcaacgcg ccacgactgt acagtccctg ccagaaaaaa
    18661 ttctttcgtg ggtctgtatc ggggcaacct taaccgcaat tgtgatcatc cttttagctg
    18721 cgtcgctccc gcctgtggat tttgatgttc gcgaatacca tctccaatgt ccgaaagagt
    18781 ttttccagca gggtcgtatc acttttttgc cgcacaacgt ttacggaaac atgccactgg
    18841 gtctggaaat gcacgccctg ctcgggatgc tccttcttaa cgatttttgg tacggggcct
    18901 tggcgggcaa ggttgtcatg gcaacattcg cgttaattgg ggcggcagcc ttactccact
    18961 gggcgcgatc aagaggtgcg agttcccctt gggtagcacc agccattttt atctcagtcc
    19021 cctgggtcat ctcggtctcc acggccgggc tgaacgacat tgggctcgcc gtttacatat
    19081 tcacggcttt ttgtgccgct tcaaagctcg tctcgatcct gaccgacgcg gatcaactct
    19141 atccggcact cgaacgaggg gctgattggg ccactatcac gagggagaat caacagcctg
    19201 aaaggaaccc attgaacaag ggatgtccca tccgccggga aagctgggcc tgggtgatca
    19261 ttgccggata cctggctggc gcggccgccg cgtgcaaata cacgggcttg ctattcggag
    19321 gctttttgat cgggatgttt ctgttggtaa cgttggccca tcgcatcccc gtacggtcgc
    19381 tcttgctgat gcttggcctc tacggattgg gaataatcct cggtgcaggc ggctggtaca
    19441 ttaagaacct cgttttcagt ggtaacccgg tttaccccct tgcttacgag tggctgggcg
    19501 gcacgtcatg gaatcccgaa aaggccgcac gctgggcacg aatccacagc ccccacgatt
    19561 tttccgttcg gatgttactg gtcgatctcg gccgggtttt tttcaccagc gaatggcaaa
    19621 gtccccttat cacaccactg gcattcttgg gtctcttgag ggtcagtaaa caaagacttt
    19681 tcgaaaaagc cacagcggtc atggcgatag tccttttttt actttggtgg tgcttgaccc
    19741 accgaattga ccggttttgg cttcctatgt tgcccttttt gtgtgttttg gcaactgtcg
    19801 gatttgactg gacggaccga ctatggtggc gttggtttgt taccgggatt tgtcttatcg
    19861 ccatgatttg caatgggttg gtaataacga gcggagctgc ggccgataat cgctattttg
    19921 ccccgcttgc gcagttgcgc aacgacccag gtcgtattgg tgcggatcat gcccttctga
    19981 acgagctttt cgcaagagat cacgctgagg gcaagaagaa gaaacttttg gccgttggcg
    20041 aggccgccgt tttcgatttg gaaatgccag tcctttacgc cacctgcttc gacgatcaac
    20101 cgtgggagat tctgaccaaa ggcaagaccc caaaagaggt tagggagacg ctccaccgag
    20161 aaaacgtcgg ctacctctat gtcaactggc tggaaattgc gcggtatcga tcgcccggaa
    20221 actacggttt taccgagttt gtccagcctg agcaatttcg tgagttaacg cgggccggcg
    20281 tcctcgttcc cgtcttaatg ccgacgcgca gccccaatca gatctacctc gttccttaaa
    20341 cccgaccacc cgtgtcggaa gaggcgaagg aaatagcccg acttttcacc aagtttccgt
    20401 taaactttgc cgattattcc gacagcggcg aatacagaga gagtatcgcc tgatccggtt
    20461 gtacgggttg actaagccct tccgctggaa ctcaaaagga cactgcccaa ccatggtccc
    20521 cctggattac agcaaaaaat acttgcgata ccccaaaaaa acagatttcc gctgcccaga
    20581 gaatcaatta ccacgtttat cgaacaagtg gggattgggc tcggctgcgt gtttctgcat
    20641 ccttttgaca ctctttctcg ccgtgggctg tcgcaccatg gacttctacg accggggtct
    20701 ggagcaaccg gccccgaccc aggagttgga acccccgcga gagttgtcta aggtctctct
    20761 ccccgcctat cgcctggaac cgcccgatgt cgtccagatc gaggtcccca agatggtccc
//
LOCUS       DAOSUT010000070        20545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1688_length_20545_cov_10.270669, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000070 DAOSUT010000000
VERSION     DAOSUT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20545
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1688_length_20545_cov_10.270669"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLD05_08160"
     CDS             <1..421
                     /locus_tag="PLD05_08160"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOP77452.1"
                     /translation="KGRFIGVVIGVRNLHPGQWWGEGEIKAYMDGDQEFPTICGTGSE
                     DYVGLSWGMQQVPFFYQGCSLNEKNFVSMYRWHLPDPIAWQKECRITIQQIGWNKGLV
                     ETQDDWCCATFWYEPTPSAPLPEMPDVAARIADIWTD"
     gene            563..1102
                     /locus_tag="PLD05_08165"
     CDS             563..1102
                     /locus_tag="PLD05_08165"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP77453.1"
                     /translation="MNRPKLQDPDPAITSAACRSAEITYRHTVRESDRLAVVNMCRET
                     GFFHEGEVAVAEELVVERLAKGPSSGYEFIFAERNGTVVGYVCFGPIACTQSSWDLYW
                     IVVSPHMQRMGLGGALLRETEQAVKHRGGTRIYIETSGREQYRPTRNFYEKHGYTVAA
                     VLTDFYAPGDDKIVYVKVL"
     gene            complement(1141..2199)
                     /locus_tag="PLD05_08170"
     CDS             complement(1141..2199)
                     /locus_tag="PLD05_08170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOP77454.1"
                     /translation="MNWETGLSPISTSIVLITVVALLLGLVTRRFVVYRDRHGGHEGG
                     TEGIVSRLGRFIYGLGLTLGDLKRDVARQGLALDQARGELADRASGTLDLGTILSTLA
                     FIANENRRLESRLQAAESQLQEQSRQLEKQWMEVRTDPLTGLANRRVFDLELSRRLRE
                     YRTHGTSFALALYDIDHFKQINDSGGHKTGDEILVHVARAMQSAAPEGALIARIGGEE
                     FAALLPDRGLSETLALAERIRVGPGTAFDGAPALSTTLSCGVAVCQTSDTETSLFERA
                     DRALYAAKRSGRNAMFYDSGDRSVRYTKLGAGPTACVPGLHECSEDEAAIAWYDDPEA
                     RALVDVLRRKLSDLRILG"
     gene            complement(2429..3208)
                     /locus_tag="PLD05_08175"
     CDS             complement(2429..3208)
                     /locus_tag="PLD05_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOP77455.1"
                     /translation="MAKKHRVPELWIGTSGWTYDDWNGVFYPPDVKASARLDFYVQQF
                     NAVELNATFYRYPTANMITSWNRRMPETFHLAVKASRRITHLKRLRDCAAELTEFAVR
                     ISELRPLRVILWQLPPSMRPDLTLLSAFLEGLSRSILSGVRHALEFRHKEWWNDEVRE
                     LLCKYRVAFVAVSHPSLPSQIVPTTDFLYIRFHGLGRQLYRYDYSEAELHDWAKRVAE
                     VCCVADVSNVYTFFNNDFHAYAVKNAQTFRELLRGELRTAG"
     gene            3454..4617
                     /locus_tag="PLD05_08180"
     CDS             3454..4617
                     /locus_tag="PLD05_08180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOP77456.1"
                     /translation="MRWNFLGKLWITLGVVGVFGWFDAGSAIRAESRTATPPSATVQQ
                     VGPKEAAFRKQLDAWINLLEQIREIRVKYPNANAAEMAQLREKYEQLLNEAEKQLPSL
                     IAAAEAAYDERPQTEGDLVDLLLEVALDRSEQGPFQDGLALAEKLIKNKVTSPVVYLA
                     AGRAAFALEDFTKAAGYFKKISEAGIQDEQVKAMEDAANFYAQQFPLEQQKRQAEAKA
                     DDLPRVLLKTTKGDILLELFENEAPNTVANFITLVEQGFYNGLTFHRVIPGFVAQGGC
                     PKGDGTGGPGYQIADECNGPNARLHFRGSLSMANAGPNTNGSQFFITYAPTSHLNGKH
                     TVFGRVLSGMDVVEKLQARNPEAPNPPEPDKILSATVVRKRPHPYTVKKLEKS"
     gene            complement(4667..4972)
                     /locus_tag="PLD05_08185"
     CDS             complement(4667..4972)
                     /locus_tag="PLD05_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77457.1"
                     /translation="MIRSKSLSQSALSLWERVGVRASDRRSLSRKRAAIRATLTPGPS
                     PAERERGGCGIGSETAGKERPGEHSTQPGKKERYRLIVASVILIAWIISLMLMASLT"
     gene            complement(4990..5484)
                     /locus_tag="PLD05_08190"
     CDS             complement(4990..5484)
                     /locus_tag="PLD05_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77458.1"
                     /translation="MAGHASPLPRALLELLKEDPRYKLDAYLFIFQALDFARQLGMGR
                     EAPSEPLPDEVRERFREISGHVADEDEVEEEPPKHISGQELCEAARLYASQQFGYLAK
                     TVLNEWGIYSTSDFGEIVYNLIRIGLMRKTKEDRREDFDNVYDFEEAFCRNYRPGRGV
                     GQFE"
     gene            complement(5727..6248)
                     /gene="aroH"
                     /locus_tag="PLD05_08195"
     CDS             complement(5727..6248)
                     /gene="aroH"
                     /locus_tag="PLD05_08195"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF019356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOP77459.1"
                     /translation="MMYCRGVRGATTADANTPEAILEATRQLLALMIRQNGIAPEDIA
                     AAFFSTSPDLNAEFPALAARQLGWMSVAMLCSHELDVPQSLQRCIRVLILWNTTKKAD
                     EIVHVYIKGAEKLRPDLSSLPPVDWQELEEWIAAHLNQEAQPSGREQGSSGGGVSPNL
                     GGADGIRGPSCAK"
     gene            complement(6355..8505)
                     /locus_tag="PLD05_08200"
     CDS             complement(6355..8505)
                     /locus_tag="PLD05_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77460.1"
                     /translation="MAIDRFSMCPGGTGKKIKFCHGDCVGFLEKVQKLMDGGQIRAAL
                     QLVDQTLSKDPSRSCVWSYKCLLERAVGNVDQFVKAADEYSQRFPKSPVALAELSLSH
                     LFRGQFSNAIDSLGQSLIASEEQKIIYVRQTAAAATLSEALIKFGHIPPAVEILMLFQ
                     NVSHNGQTSVQELLGQVIASQEIPLELREAQWDGLFMDEKYQQLLEVTDRLAVRLRWR
                     DLEKYLRDYLSSNPEDARVWHFLGVTLMWLMEPQEAATAFTRAGELEKNDRLAVFSLY
                     RSFLVTGALGDEENSYDVEVIVDEPDRLKEAVLSDRLVFSQRPEHGQPLPWYFPDDVG
                     EEITPELILAVLRRSIGGDGSQNGRVFSEPGKTPLIAFGSFYGRQTDRPARLLFAGVY
                     ESDLERLKEMVKGWLGREDVEWSIKPGSDESSRFIRLALDGEVIAGFDRSVDLKEEWK
                     TPIFEWLPNMTFPALGGLSLKEAANDPQKRRWALAILKYIDYRILNTTITGYLDELRQ
                     QLGLGFVDELDVDIKRFRRIPLSLVKVLDLTRLNIETLTQFFNLALVYRESTLIYELA
                     SEIVRRRDEDGVPAALVRTAYIGAIDEALNLGIDDDQIKRLTEEGLAFLEKHNLPHGS
                     LHVRQFRMAFSHGNSEEAKPLLDHMFREHEDEADVMEFLRQFMSLLGYFEQLAQQEGQ
                     SPTAGRPSGGGIWTPDSEAGRPGSSKKLWVPGSD"
     gene            9232..10668
                     /locus_tag="PLD05_08205"
     CDS             9232..10668
                     /locus_tag="PLD05_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77461.1"
                     /translation="MFRMLSKAILLATVIFSLPGWSVASTEVQATRWNVIFILLDDLG
                     ATDFGCTGSRFYETPHIDRLAAQGMRFTQGYAACTVCSPTRAAVMTGKYPARLHITDW
                     IKGHVRPKAKLRIPDWTMYLPLEEVTIAERLRLAGYATAHVGKWHLGDPPYYPEHQGF
                     DINIGGTHMGAPGSYFWPYGKDDRRVPLDGGQEGEYLTDRLTDEAIRFIEQHKDRPFF
                     LYFAHYAVHTPLQAKPELVAYYEKKLAEAAAKGDNFDGQQCPVYAAMIHSVDENMGRL
                     LECLDRLDLAERTVIFLTSDNGGLVLPACQNRSVTHNIGIRAGKGSAYEGGVRVPWIV
                     RWPGVVAPGSLCHEPVISIDAYPTILEITGVADAEGHICDGVSLVPLLKQQGNFPKRA
                     LYWHYPHYHPGGATPYSAVRDGDWKLIEFFEDHHVELYNLKDDPQESQDLASQLPEKA
                     ESLRRQLHRWREEVGAQLPTPNPDYQPE"
     gene            10766..11464
                     /locus_tag="PLD05_08210"
     CDS             10766..11464
                     /locus_tag="PLD05_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP77462.1"
                     /translation="MKTHLWLSVHALLVGLALAYAVKAEEGWITIPIEKGLPGWKLKG
                     QPEQSKWQVGTAAVDPNDPKSLICQPGGHELVNVGSRGVDIFTEQTFGDAIIELEVMV
                     PQGSNSGIYVMGEYEIQILDSYGKQTLGMGDMGAVYSMAVPKVNACKKPGEWQKYVIE
                     YRAPKFDATGTKIANGRLVKVILNDQVIHENLELKSQTPGGVTGKEAPRGPLMFQGNH
                     GPVAYRNIRIKPLN"
     gene            complement(11829..13157)
                     /locus_tag="PLD05_08215"
     CDS             complement(11829..13157)
                     /locus_tag="PLD05_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77463.1"
                     /translation="MRKYTVFLALVWSLLIVGLVGCPKPQAPEKPPAEGDLQAKETVQ
                     VNPADVEALVKRVEQLGGKIEYDQNKNPVAVDLFQRQATDEDLALIAKVPTIRRVSLW
                     GAEIHDTGIQELTKLPQLEWLVLENTNITNDGVKTLGQIKSLRSLNLRRSTNLTDEAL
                     QHLSGLTNLEELVLLYNNFSDAGMKHVANLKNLRNLDIRGCVMITDVGLEAIKDLQEL
                     RALKLRNQASVTDQGVAIIAQLRNLKDLALEDSGITDQGIEALTKLSNLENLNLMRCY
                     GVTDDGVAKLKVFTKLRRLFLRGLYIAGHGLRELKDCKNLEELDLAETQADDATAEAL
                     LNFPKLKILDLWHTQVTDTGLESIGKLVDLEELILDGTPISDAGLTHLTGLGKLRVLS
                     IKECNKVTDAATDHLSKLQSLKKLSVDQSGYTEDGVAQLRKSLPNCEVSF"
     gene            13590..>13825
                     /locus_tag="PLD05_08220"
     CDS             13590..>13825
                     /locus_tag="PLD05_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77464.1"
                     /translation="MCQQHQCSTCFNRRDCLKMVSLAAMGTGFLASLGQEPSANEGPG
                     LTDYVDVKTLRPQPDVRVAATFLELPRPYWLGWPG"
     assembly_gap    13826..13925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13926..14930
                     /locus_tag="PLD05_08225"
     CDS             <13926..14930
                     /locus_tag="PLD05_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77465.1"
                     /translation="VGTAFTGHLAGPSRKTGVFVISSLDWSAVEMGLRMIRAKRMFEE
                     TRVLWIRGNQRNETVVERLGTKVLAIPRDTFNQEFDKQPVTDEIRDLAADLRKHARKI
                     VEPNEEDTINAMRAYATAKRLLARENAHALSMDCLGMVGAKLVPTPPCGAWTLLQDAG
                     ITAGCEADLYGAMSLMMTSYLLDRPGYMNDPVPETVHNTLIAAHCTSGTRIAGFDQPP
                     ADYVLRNHSESALGVAMQVLWPEGQPITLVRFTGPNEMIIDTGKVVGNVQTPPAGGCR
                     TSVEIELDGIEDSRDTLGFHQVVTLGSHRRVLEGFCQLYGIKVTHSPRFSTFQYGGQA
                     "
     gene            14927..16078
                     /locus_tag="PLD05_08230"
     CDS             14927..16078
                     /locus_tag="PLD05_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77466.1"
                     /translation="MIRHVVFSLILAAACASLSLAEVVEFTSPGFPPSRMDAEGRLTE
                     DWGSLKIIVEGDGVMAQGGTVQVVRLEELVPAAVWSCEAGPIRVTATAYRAPVFPSGM
                     DVLTVTLQETAGQDRHVTLKMVVNPAPRVGLSTAQVEKRTVLAIPLETQQLLKLRDWG
                     YTIDTTAMPGWAKPEGECDPAFANIRAGMGGIPIRYRFRVTRRSQANVVLGICESYWD
                     RPQIRPLICLVEGARPMVVDPIDRWGRHQPGTLLFTALDNDGDGWITVTIKPVPGARD
                     QNPILNVIWVFSGDQRPNLARVAKGELNEQALYYVDVGGVRDQPILESQDLSFPVELA
                     ANSSKKLTFYVACPGGSVVTPELTAWTTESLFQAAKDVWKNWSQSVTAR"
     gene            complement(16221..18386)
                     /locus_tag="PLD05_08235"
     CDS             complement(16221..18386)
                     /locus_tag="PLD05_08235"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP77467.1"
                     /translation="MRSLSLVLSLVLSASGLGFSTGHAAEAPRPNIIIILSDDMGFSD
                     LGCYGGEIQTPNLDRLAANGLRFTQFYNTARCCPTRACLLTGVYPHQAGVGHMMEDRG
                     YDGYRGDLNRQTVTIAEVLRTAGYRNYAVGKWHVTKATKPEGPKDNWPLQRGFDRFYG
                     TITGAGSYFDPGTLTRDNTMISPFADPEYKPSVFYYTDAISDHAVRFIREHVATARTS
                     PFFMYVAYTAAHWPLHALEEDIAKYRGRYDGGYEPIRRARFERLKQMGLISPNWELSP
                     QVGDWDKVEHKEWEIRCMEVYAAQIDRMDRGIGRILEALRETGQWENTIVFYLQDNGA
                     CQEAIGRTGTWRRPVEPSLPPIALDAIRTDVRPSQNRRGVPTLSGPFIMPGPEDTYIS
                     YGINWANVSNTPFREYKHFVHEGGIATPLIVHWPAGLTRRGDLEHQPGHLIDLMATCV
                     ELAGAQYPTEFQGHKIIPMEGVSLVPLFKGQPIKRKEPIFFEHEGNRAVRDGKWKLVA
                     KENQPWELYDMEADRTEMHDLSAQYPEVVARLAAAWDEWAKRANVLPLGAWRAETKDT
                     FSTKTQFVLAPGAQLPRTEAPYVVGKGLKITAEIQAVGTSGVIVAQGGSAHGYALYIQ
                     DGKPCFAVRHKNQLKTVVGSQPLSQGPGVLSVTWSNDVVKILWNKAVLAEAKEIPPLT
                     NMPQDGLQIGRDDNGLVGDYPEENAFSGKLGKVIIEVLP"
     gene            18680..19669
                     /locus_tag="PLD05_08240"
     CDS             18680..19669
                     /locus_tag="PLD05_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77468.1"
                     /translation="MMTGVLSLDHLVVADWLSWLQTLIAMLPVWMKPLWIVLVGFLAG
                     FFLLWVVLQLMAQFAPRVTAIMRTTSKEATSQPLFYILLAIGVTMLLLSPIMPYNTFG
                     EDIKLLKAEGLTLIKLLAIILAVWTASVSISEEVEGRTALTVLSKPVARWKMLVGKFL
                     GVILPVIVMFLILGSLLLCAVSYKVKYDAKEVARPDPTVQECRDEMLQTVPGLGLAFL
                     EAVVMASIAAAISTRFPLIPNLLICASIYALGHLLPTVVIETADRLEFVAFVAKLLAV
                     ILPGLEFFSMETAIATGRSVSASYLGLAALYSLVYISLALLLGLLLFEDRDLA"
     gene            19986..>20545
                     /locus_tag="PLD05_08245"
     CDS             19986..>20545
                     /locus_tag="PLD05_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77469.1"
                     /translation="MSHVTEIKTFDELVGHRLVWRKLWGETPNASFFQSYEWFETYWQ
                     HFGKEQTLRVLVVHSCDEVIGLLPLVVRTERTRVGPVRVLTYPLHDWGSFYSPIGKNP
                     TATLLAGLRYVKESSRDWDILDLRWVDRFGQDRMRTPTAMRQVGFEPSEQVWMQSAIV
                     SIDESWESYWASRTSKFRNNIRRAEKR"
BASE COUNT         4558 a         5761 c         5496 g         4630 t
ORIGIN      
        1 caagggacga ttcatcggag tggttattgg ggttcgaaat ctccatcctg gccaatggtg
       61 gggcgaaggt gaaataaagg cgtatatgga tggcgaccaa gagtttccca ctatttgtgg
      121 cacgggcagt gaagactatg tgggcctctc atggggaatg cagcaagtgc ctttctttta
      181 tcaggggtgc tcgctcaatg aaaagaactt tgtctccatg tatcgttggc atttacctga
      241 tcccattgca tggcagaagg aatgccgaat caccatccag caaattggct ggaacaaagg
      301 ccttgtagaa acccaggatg attggtgctg cgccaccttc tggtatgagc caacgcccag
      361 tgctccgctg cccgagatgc cagatgtcgc tgccagaatt gccgatatct ggactgatta
      421 attcgtggga cttgggggcc agtcacagaa ccgtcggcac acggcacgcg tatgccgact
      481 tggggagtct gtctgcacag gtcgatcgaa ccacagtcgc gttgaatccc cgcaggaaac
      541 atgttagccg agaaaggatt ctatgaatcg tccgaaatta caagatcctg accccgcaat
      601 aacgtcggcc gcatgccgct ccgccgagat tacctaccgg cataccgtgc gagaaagtga
      661 ccgcttggcc gtggttaaca tgtgccgcga gaccggcttc tttcacgaag gcgaggtggc
      721 tgtcgccgag gagctggtcg tcgagcgact cgccaaagga ccaagcagcg ggtacgaatt
      781 cattttcgct gagcgaaatg gcaccgttgt gggctatgtc tgctttggtc ctatcgcttg
      841 tacccaatcg agttgggatt tgtattggat tgttgtatcc ccacacatgc agcgaatggg
      901 cctgggcggt gctctgttga gagaaacaga gcaagccgtc aaacatcggg gcgggacacg
      961 catttacatt gaaacatcgg gacgcgagca atatcggcca acgcggaact tttacgagaa
     1021 gcacgggtac accgtggccg cggtcttgac agacttttac gcacccgggg atgacaagat
     1081 cgtctatgtg aaggtcctgt aaaaaaggta gcccgttgcg gtcctgctcg cttgattgtt
     1141 tcaccctaaa atccgcaagt ccgacagctt tcgccgcaag acgtcaacca aggccctggc
     1201 ctccgggtcg tcataccagg ctatggccgc ttcatcttcc gaacattcat gtaagcccgg
     1261 cacgcaagcc gtggggcctg cgcccagttt tgtgtatcgc acacttcgat caccggagtc
     1321 atagaacatg gcattacgac ccgatcgttt tgcggcataa agggcccgat cagcccgttc
     1381 aaaaagactc gtttctgtat ccgacgtctg acacacggcg acaccacaac tgagcgtcgt
     1441 tgatagggcg ggagcaccat caaaggccgt acccggccca acacggatcc gctctgccaa
     1501 ggccagggtt tcgctcagtc cacgatcggg caacagggct gcgaactcct caccgccaat
     1561 ccgagcgatc aaagcaccct ctggggccgc cgactgcatg gcgcgggcaa cgtggacaag
     1621 gatctcatcg cccgtcttgt gtcccccact gtcattgatc tgcttaaagt ggtcaatgtc
     1681 ataaagggcg agggcgaatg atgtgccgtg cgttcgatac tccctcaatc tgcggctaag
     1741 ttctaggtcg aaaaccctgc gattggccag accggtcaag ggatcagttc gcacctccat
     1801 ccattgtttt tcaagttgtc ttgactgctc ctgcaattgt gactccgcag cttgaagtcg
     1861 gctctctaat cgccgattct cattggcaat gaaggctaat gtactgagga tcgttcctaa
     1921 atccagtgtt ccgctggccc ggtcggcaag ttcgcccctc gcctggtcga gcgccaaacc
     1981 ctgccgcgcg acatcccttt taaggtcacc caacgttagc cctaaaccat aaatgaatcg
     2041 gcccaatctg ctgacgatgc cctcggttcc tccttcatgg cctccatgac gatcccggta
     2101 aaccacgaat cgccgggtga ccaacccaag tagcaaggct accacggtga taagaacaat
     2161 gctggtgctg atgggagaca atcctgtttc ccagttcatt tggacacctt gatccacatt
     2221 gagcgatctc agtgtagcta aaacacgtca catcggggaa ggttccaaga aaagccttcc
     2281 cccgaaacgg tttcttgccg tttcatgtcc tcctatgaaa acctacattt ttgaggagac
     2341 cgttgtccaa cgtttgtgaa caatagaaag acgtccgctg cgacaagttt gcgagttttg
     2401 caagaaatgc caaaagaatt gtccaggctt atcctgccgt tcttaattct cccctgagaa
     2461 gctcacgaaa agtttgggca ttttttaccg cgtaagcgtg aaaatcattg ttaaaaaaag
     2521 tatacacgtt gctaacatcg gcaacacaac atacctcggc aacgcgcttg gcccaatcgt
     2581 gcaactcggc ttccgaataa tcgtagcgat acaactgccg tcccaaccca tggaaacgaa
     2641 tgtacaagaa atccgtggtc gggacgattt gtgatggcaa actgggatgg ctaaccgcca
     2701 caaacgccac ccgatacttg cacaacagtt ccctgacctc gtcattccac cactctttgt
     2761 gacggaactc cagggcatgg cgaactccgg agaggatcga tcgactgagc ccctcgagaa
     2821 atgccgaaag taaggtgaga tctggcctca ttgacggcgg cagttgccaa agaatgaccc
     2881 gcaggggacg caattcactg atcctcacgg caaattcggt cagttctgcg gcacaatcgc
     2941 gaagtcgctt aagatgcgtg attcgccggg aggccttaac cgcgaggtgg aacgtttccg
     3001 gcatccgccg gttccacgag gtaatcatgt ttgccgtggg atagcggtaa aaggtcgcgt
     3061 tcagctcaac ggcgttgaat tgctggacgt aaaaatcgag tcgggccgat gccttcacgt
     3121 ccggcggata aaaaactccg ttccagtcgt cgtaagtcca accggaagtt ccaatccaca
     3181 actcggggac acgatgtttc ttggccattg acgacgctca ctctaacgac tccagaattg
     3241 ccgtcccgtt aaagcgggag caaatctcgc gaaactgttt ccacttgccc ttcttgacgc
     3301 caatctttgg acgcgcaaca cgtttgtccc gtattatctt ccgctcgcaa ccccttctgg
     3361 ggatttctgc tacaatgaaa agtgaactct cggggaaagt caagcttgcc ccgtttcgtt
     3421 tcctgtccct atttgacgaa aggacgatac gtgatgcgat ggaatttcct tggaaaattg
     3481 tggatcacgc tgggtgtggt aggtgttttt ggatggtttg acgccggttc ggcaatccgg
     3541 gccgaatcta gaacggccac cccgcccagc gcgactgtcc aacaagttgg cccgaaagag
     3601 gcagctttcc gtaagcaact cgacgcgtgg attaacctgc ttgagcagat tcgggaaatc
     3661 cgcgtgaagt atccaaacgc caatgccgcg gaaatggccc aattgcgaga gaagtatgag
     3721 cagttattga atgaggctga aaaacagttg ccctctttga ttgcagcggc ggaggccgcc
     3781 tatgatgaac gcccccagac cgagggcgat cttgtcgatc tccttttgga ggtggccctg
     3841 gaccgaagtg agcaagggcc ttttcaagat ggtctggccc tggccgagaa gttgattaag
     3901 aacaaggtca cgtcgccggt tgtttatctc gcggctggac gggcggcctt cgcgttggag
     3961 gatttcacca aggccgcagg ctatttcaag aaaatcagtg aagcaggaat tcaagacgaa
     4021 caggtgaaag cgatggaaga tgccgcaaac ttttatgctc aacagtttcc gctcgaacag
     4081 caaaagcgac aggccgaggc aaaggccgac gatctgccgc gagtcctttt gaaaacgacc
     4141 aagggtgaca ttttgctgga gctcttcgaa aacgaagccc ccaacacggt ggccaatttc
     4201 atcacccttg tggagcaggg gttttacaac gggctaacgt tccaccgtgt catccccggt
     4261 ttcgtggctc aagggggatg tcccaagggt gacggcacag gtggaccggg ctatcagatt
     4321 gcggatgaat gcaacggccc caacgctcgt ctgcacttcc ggggtagtct cagcatggcc
     4381 aacgccgggc cgaataccaa cggctcacag ttcttcatta catatgcccc aaccagccac
     4441 ttgaacggta aacacaccgt ctttggtcgt gttctctcag gaatggatgt ggtggagaaa
     4501 cttcaagcgc ggaatcccga agccccgaat ccaccggagc ccgacaagat tttatccgcg
     4561 actgtggtac ggaaacgtcc ccatccctac acggtgaaga aactcgaaaa atcgtgatct
     4621 actgaccacg ccagacctgg ttcgttcgct gatggtcagt ctccgcctat gtgagactgg
     4681 ccatcagcat caggctgatg atccaggcga tgagaatgac cgaggcgaca atcaaacggt
     4741 accgctcctt tttgccgggc tgagtcgaat gttcgcccgg tcgttctttc cccgctgtct
     4801 cagagcctat cccacaacct cccctctccc gttcggcggg agaggggccg ggggtgaggg
     4861 tggctcgaat cgctgcccgt tttctcgata gactccgacg gtcacttgcc ctcaccccaa
     4921 ccctctccca gagggagagg gcactttggg ataggctctt ggaacgaatc atcttcgcca
     4981 ctccggggtt cattcaaatt ggcctactcc cctaccggga cggtagtttc ggcagaaggc
     5041 ctcttcaaag tcgtaaacat tgtcaaagtc ttcgcgtcga tcttctttgg tttttcgcat
     5101 gaggcctatt cgaatcaggt tatagacgat ctcgccgaaa tcactcgtgg agtagattcc
     5161 ccattcattg agcacggtct ttgcgagata gccaaattgc tggctggcat acagcctggc
     5221 ggcctcgcaa agctcctggc cggaaatatg cttcggcggt tcctcctcca cctcgtcttc
     5281 atccgcgacg tgaccactga tctcccgaaa gcgctcccgg acctcgtcgg gcaagggttc
     5341 actgggcgct tcacgcccca tgcccaattg ccgcgcaaaa tcgagtgcct ggaagatgaa
     5401 gagataggca tccaacttgt agcgtggatc ttccttcaaa agttccaaga gcgctcgtgg
     5461 taaaggcgac gcgtgtccgg ccatggcttc ttgacaacgt gtattaaagc gtatcccaca
     5521 acctcccctc tcccgttcgg cgggagaggg gccgggggtg aggatggctt gaatcgctgg
     5581 cgttttctcg atcgactccg acgatggctt gccctcaccc caaccctctc ccaaagggag
     5641 agggaattct gaaataggct cattagcaaa cggctctcag tgtttcaaag tagcttacat
     5701 ctttttttcc tatacgcagc gtccggctat ttggcacacg acgggccgcg gataccatcg
     5761 gcgccgccca aattggggga aacaccccca cccgaggagc cctgctcccg accgcttggt
     5821 tgggcctcct gattgagatg ggccgcgatc cactcttcga gttcttgcca atcgacgggc
     5881 gggagcgaag agaggtcggg ccgcagcttt tccgcaccct taatatacac gtgcacaatc
     5941 tcgtcggcct tcttggtcgt gttccaaagg atcaacactc ggatgcaccg ttgcagggat
     6001 tgggggacat cgagttcgtg actgcataac atggcgacgc tcatccaacc caactggcgg
     6061 gcagccaggg ctgggaactc ggcatttaag tcaggagaag tcgaaaaaaa agcagccgca
     6121 atgtcctccg gagcaattcc attttgtcga atcatgagcg ccagcaactg tcgcgttgct
     6181 tccaaaattg cctctggggt attggcatcg gcagtcgttg cgcctcgcac gcctcgacag
     6241 tacatcatgg ttcgcaactt acctccaagg acccaattcc acaagccaac gtgacgactg
     6301 atccagactc aagccacgcc atcatacggc cccgttggca ttttccggca gatgtcaatc
     6361 gcttcccggc acccagagct tcttcgatga gcctgggcga ccggcttcgg aatcaggggt
     6421 ccaaatccct ccaccggatg gacgtcccgc ggtcggggat tgaccttcct gttgcgcgag
     6481 ctgttcaaaa taccctagaa gcgacatgaa ttgccgaagg aattccatga catccgcctc
     6541 atcctcatgt tccctgaaca tgtgatcgag gagcggcttt gcctcttcgg aattaccatg
     6601 cgagaaagcc attcggaact ggcggacatg taaactaccg tgcggcaagt tgtgtttctc
     6661 aagaaacgcc aagccttctt ccgtcaggcg tttaatctgg tcgtcatcga tcccaaggtt
     6721 taatgcctca tcaatcgccc cgatataagc agttcggaca agggccgcag gaacaccgtc
     6781 ttcgtcccgt cgccgcacaa tttccgaggc caactcataa atcaaggtgg actcacggta
     6841 aaccaaagcc agattgaaaa actgcgtcag cgtttcaata ttgaggcgcg tcaagtctag
     6901 gacttttact aacgacagcg gaatccggcg gaatcgctta atgtcaacat cgagctcatc
     6961 cacaaagccc aagccaagtt gctgtctcaa ctcgtccagg taaccagtaa tcgttgtgtt
     7021 taagattcgg taatcgatgt acttgagaat ggccaaggcc caacgacgct tctgcgggtc
     7081 gttcgccgcc tctttcagcg agagaccgcc caacgccgga aacgtcatat tgggcaacca
     7141 ttcgaaaata ggggtcttcc actcttcctt gagatcaacg gaacgatcaa aacccgcaat
     7201 gacctccccg tcgagcgcca atcttataaa acgagaagac tcgtccgacc ctggttttat
     7261 tgaccattcg acatcctcgc gacccagcca ccctttgacc atctccttga gtcgctccaa
     7321 atcggattcg taaactcccg cgaaaagcaa tcttgcgggc cgatctgttt gccgcccgta
     7381 aaaactgccg aaagcaatca ggggcgtctt gccgggttcg ctaaacacgc gtccattttg
     7441 gctgccatcc cctccgattg accgacgcaa aacggcaagg ataagctccg gagtgatttc
     7501 ctcgcccaca tcatcgggga aataccatgg gagcggttgc ccgtgttccg gtctttgcga
     7561 gaaaaccaga cggtcggaaa gcacggcctc tttcaaccga tctggttcat caacgatgac
     7621 ttccacgtcg tagctatttt cttcgtcgcc taatgcaccg gtcacaagga aagaacgata
     7681 cagagagaag actgccagcc gatcgttctt ttccaattcg ccagcgcggg taaaggccgt
     7741 ggcggcctcc tgaggttcca tgagccacat caacgtcacg cctaagaaat gccacacgcg
     7801 ggcatcttct gggttactac tcagataatc tcgcagatat ttttcgaggt cccgccatcg
     7861 taaacgcaca gccagacgat cggtgacctc caaaagctgc tgatatttct catccatgaa
     7921 caagccatcc cactgggcct cccggagttc cagcggtatc tcttggctag caataacctg
     7981 gcctagtaac tcttgcacac tggtctgccc gttatggctg acgttttgga aaagcatgag
     8041 gatttccaca gcgggcggaa tgtgaccaaa cttaatgagc gcctcgctca gagtcgccgc
     8101 agccgccgtt tgcctgacat aaatgatttt ctgttcctcg ctggctatca agctttggcc
     8161 taaggagtct atggcatttg agaactggcc ccggaaaagg tggctcaggg acaattcggc
     8221 caaggccacg ggacttttcg gaaatcgctg gctgtactcg tcggcagcct taacaaattg
     8281 gtccacattc ccaactgccc gctccaaaag acacttgtag gaccaaacgc aggaacggct
     8341 cgggtctttg gacaacgttt gatcaaccag ttggagggcc gctcggatct gcccaccatc
     8401 catgagcttc tggacttttt ccaagaagcc cacacaatcg ccgtggcaaa acttgatctt
     8461 tttacccgtt ccgccgggac acatcgaaaa cctgtcaata gccatgattg ggatatctcc
     8521 tcatccgaac tttttcacgg atcgaaaaaa tttcgcaagc gtccacctag gccccgccta
     8581 ggtgttaagg acagcccgct gctgtcaatt tcaaccagcg cttttgtcag aaaacgcgac
     8641 cctccacaac acgtgaaatc caaacaaacc ggtaaatctt ttcacatttg gcttggggcc
     8701 ccgcaaaagg ccaagccttt atgttatcag gtattgcctg gcgtggggag acaatgagcg
     8761 ggccgggacg ccatttcccg gcagatcgcc tgtgagaagg ggcatacact cctaacgcgc
     8821 aggcgcgggg ccccgccctg gcgagaaccg gaaaacgcgt ccatataaag tgccccacct
     8881 tatggcgggg tccgcaattg cgatgtaggt tcccggtgtt tgacaggacc atgacggtgc
     8941 gggcccgtca gggtgaacga tgatcaaaca tcacaaatgg agggacgcac gccgtcgcat
     9001 ccgccgtcat acagccgccg gttttaaact gacagtggtt cccaaccgat tgcacgggct
     9061 cccattgatg caccacgtgt gttttattcc ccttaagggc aatctttggc tctacgcttt
     9121 ctttacgttg gcaccatcac gggaagacct tgtggacttg ccctcttggg cggagtaaac
     9181 tagggcaaac gagaacagct cgatcattga gtccgcttgg ggaggtagaa catgttccga
     9241 atgctctcaa aagctatttt gttggccacg gtcatcttca gcttgcctgg ctggtcagtg
     9301 gcgtcaacag aagttcaagc gacgcgctgg aatgtgattt tcattctgct tgatgactta
     9361 ggggccaccg atttcggatg cacaggcagt cgcttctatg agactcccca cattgatcgg
     9421 ttggctgccc agggaatgcg attcacccag ggttacgcag cctgcactgt ttgctcacca
     9481 acgcgggcgg ccgtcatgac gggaaaatat ccggcacggc ttcatattac cgactggatc
     9541 aagggacatg tccgtcccaa ggcaaaactg cgcatccccg attggacaat gtacctccct
     9601 ctcgaggagg ttacgatcgc cgagcggttg cgtttggcgg gttacgccac cgcccacgtc
     9661 ggcaaatggc atctgggcga tcctccctat tatcctgaac atcagggatt cgacatcaac
     9721 atcggcggaa cacatatggg agcccccggt agttatttct ggccgtacgg caaggacgat
     9781 cgtcgcgtgc cgcttgacgg ggggcaggag ggggaatatc ttaccgatcg gctaacggac
     9841 gaagctatcc gctttatcga acagcacaag gatcgaccct tttttctcta tttcgcccat
     9901 tatgccgttc atactccgtt gcaggccaaa ccggaattag ttgcctatta tgagaaaaag
     9961 ctggccgagg ccgctgccaa aggggacaat tttgacgggc agcagtgccc ggtttatgca
    10021 gccatgattc acagtgtgga cgagaacatg ggccgcctct tggagtgctt ggatcgactc
    10081 gacctggccg agagaaccgt gatttttctc acgtcggaca atggtggatt ggtactgccg
    10141 gcttgtcaga acaggtccgt cacgcataac atcggcatac gggcaggtaa aggctcggca
    10201 tacgaaggag gtgtccgcgt cccatggatc gttcgctggc ccggtgttgt cgctccagga
    10261 agcctttgcc acgaaccggt aatcagcatc gacgcctacc ccactatttt ggagatcacg
    10321 ggggttgccg atgccgaggg tcacatctgc gatggcgtca gtcttgtgcc gcttctgaaa
    10381 caacaaggga acttccccaa gagggccctt tattggcact atccgcatta tcatcctggc
    10441 ggtgccacgc cctacagcgc cgtgcgggat ggcgattgga aactcatcga atttttcgaa
    10501 gatcaccacg tggagcttta taatctgaag gatgatcccc aggagtccca ggacttggcc
    10561 agccagttgc cagagaaggc ggagtcactt cgcaggcaat tgcatcgctg gcgtgaggag
    10621 gttggtgccc aattacccac ccccaatccc gactatcaac cggaataacg cttcctgccg
    10681 caatgaacct cgtgtctccc agttggacgg agacatttcg agctttcgcg agtttgttcg
    10741 taccttttat gaaaggagtg agcacatgaa gacgcatctt tggttgagcg tgcacgcatt
    10801 gctcgtcggc ttggccctgg cctatgcggt aaaggcggaa gaaggctgga tcacgattcc
    10861 catcgaaaag ggtctcccgg gttggaagct gaagggtcaa ccggagcaaa gcaagtggca
    10921 agtcgggacg gccgccgtgg accccaatga tcccaagtca cttatctgtc agcccggcgg
    10981 gcatgagctt gtaaacgttg gatcacgcgg tgtggacatc ttcaccgagc agaccttcgg
    11041 agatgcgatc atcgagctgg aggtcatggt tccccaggga tcaaattccg gaatctacgt
    11101 catgggtgaa tacgagattc aaatcctcga cagctatggt aagcaaacct tgggcatggg
    11161 cgatatgggc gcggtttata gcatggccgt cccaaaggtc aacgcctgca aaaagccggg
    11221 ggaatggcaa aagtacgtca tcgagtaccg tgctcccaag ttcgacgcca cgggtacaaa
    11281 aattgcgaac ggccgtctcg tgaaggtcat cctcaacgat caggtcatcc acgagaatct
    11341 tgaactcaag tcacaaacgc ccggcggtgt caccggtaaa gaggcacccc gtgggcccct
    11401 gatgttccag ggcaaccacg gaccggtggc ttatcgaaac atccgtatta agccgctaaa
    11461 ttgaagacca atcgaaggct acaagtaatc gcttacgaaa tcgtgccggc ccggggtggc
    11521 gcgctgtttt tgcatcgttt cgatgagccg cttcgatagc aaactcaata agaaggcccc
    11581 ttcggcgaga gaccgaaggg gctttgattc agagcccatc ccaaagttcc ctctctctgg
    11641 gagagggtta gggtgagggc aagcgatcgt cggagtcgat cgagaaaacc agcagcgctt
    11701 cgagccactc tcacccccgc cccctctccc ggcgaacggg agaggggagg ttgtgggata
    11761 agctcttgtt tagaccccgt tttgcatttc gtatcttttc tgacgggcaa agccccatga
    11821 gttaccgttc agaagctcac ttcacaatta ggcaacgact tgcgaagttg ggcaactcca
    11881 tcttctgtgt agccactttg atccacagac agcttcttga gactctgcaa tttagaaaga
    11941 tgatccgtcg cagcgtccgt gactttgttg cattccttga ttgataacac cctgagttta
    12001 cctagaccgg tcagatgagt caggccagca tcactgatgg gagtcccgtc caaaatgagc
    12061 tcctccagat caaccagttt gccgatactt tccaatccag tgtccgtcac ctgcgtgtgc
    12121 cacaggtcta ggatctttag cttggggaaa ttcagcaaag cctcggccgt agcatcatct
    12181 gcctgagtct cggccaggtc cagctcctcc aagttcttgc agtccttgag ctctcgcaga
    12241 ccgtgaccgg caatgtaaag tcctcgtaga aagagccgac gcagcttagt gaagaccttc
    12301 aacttggcca cgccgtcatc tgtgaccccg taacaccgca tcaggttcag attctccaga
    12361 ttgctcagtt tggtcaaagc ctcaatgcct tgatcggtga tgccactatc ttccaaggcc
    12421 aaatccttga ggttcctcag ctgagcaatg atggcaacac cctgatcggt aaccgaggcc
    12481 tgattccgaa gctttagggc acgaagctcc tgcaggtctt taatcgcctc gaggccgacg
    12541 tcggtgatca tcacacagcc tcggatgtcg agattgcgaa ggttcttcag attagcgaca
    12601 tgcttcatac cagcatcgga aaaattgttg taaagcagga ccaactcttc aagatttgtt
    12661 aaaccagaca agtgctgcag ggcttcgtcc gtaagattcg tactccgccg caaattgagg
    12721 gagcggaggc ttttgatttg cccgagagtc ttgacaccgt cattcgtgat gtttgtattc
    12781 tctaacacaa gccattcgag ttgcggaagc ttggtcagct cctgaattcc ggtgtcatga
    12841 atctcggccc cccacaggct gacccttcga atggtaggaa cctttgcgat gagggccaaa
    12901 tcctcatcgg ttgcttgccg ctggaagaga tcaaccgcaa ccgggttctt gttttggtca
    12961 tactcaatct tgccgccgag ttgctccaca cgcttgacga gcgcttccac gtcggccgga
    13021 ttaacttgca cggtctcctt ggcctggaga tcgccttcgg cgggtggctt ttcgggcgcc
    13081 tggggtttag ggcatccgac caaaccaacg atcagcaggc tccatacgag agccaagaaa
    13141 acggtgtact tccgcatgaa tcacctctct tagaaacgca cgtctcaatg ctcgaccgtt
    13201 atcgctcgat tgtgtccacg cttgcgctgg cgatgtccca aaccttttac gttatcatga
    13261 ggcccagaag ttcgcaaacg ggtcacccgc ggtgaatttg taaaggtttt tctgagcgat
    13321 ttgcagggac acacgctcag gttgcactca atctgctaag gacgcgctct cgcttctgcc
    13381 gttgtgccaa tcgatcattc atggttgacc cagccacgat ggcgcgtagc cccccaacac
    13441 aatatgcttc atgcccattg ttgcagatag cctcgggaaa ggttactatt ctgtcggcgg
    13501 cagattgggg aatcttcggc cgcctcgttt tcggtaacag ttcggcgact tttcacgcat
    13561 cgttcggtgg ttgactggga ggacgcgata tgtgtcagca acatcaatgt tcgacgtgtt
    13621 tcaaccggcg ggactgtctg aagatggtct ccttggcggc gatgggaacg ggattcctgg
    13681 cctcattggg ccaagaacca tcggccaatg aaggaccggg cttgacggat tatgtcgatg
    13741 tgaagacact tcggccacag cccgacgtgc gtgtggcggc caccttcctg gaactcccgc
    13801 gtccttactg gcttggctgg cctggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnngtggg cacggccttt acgggacatt tggcaggccc aagtcggaag acgggtgttt
    13981 tcgtcatctc ctcgctcgac tggtcggctg tcgaaatggg attgcggatg atccgggcta
    14041 aacgcatgtt tgaggagacg cgagtcctgt ggattcgcgg gaatcagcga aacgagaccg
    14101 tcgtagaacg tcttgggaca aaggttctcg ccattccccg agatacgttt aatcaagagt
    14161 tcgacaaaca gcccgtcacc gacgaaattc gcgatctcgc cgcggacctg cgaaagcacg
    14221 cacgaaagat tgtggaaccc aacgaagagg atacgatcaa cgcgatgcgg gcatacgcca
    14281 cagccaagag gcttttggcc agggaaaatg cccacgcttt gagcatggat tgccttggca
    14341 tggtgggcgc aaagctagtg cccacaccgc cttgtggggc gtggacgctc ctccaagacg
    14401 ccggtattac agccgggtgc gaagctgacc tgtatggcgc catgtctctg atgatgacca
    14461 gctatctgct cgatcgtccc ggctacatga atgatcctgt gcccgagacc gtacacaaca
    14521 ccctgattgc ggcccactgc acgtcgggaa cccggatcgc tgggtttgac caaccgccgg
    14581 ccgattatgt tctccgtaac cattcggagt cggcccttgg cgtggccatg caggtccttt
    14641 ggcccgaggg ccagcccatc acgctggttc gcttcaccgg accaaatgag atgatcatcg
    14701 acaccggcaa ggtcgtaggc aatgtccaaa caccgcccgc cggtgggtgc cgaacctccg
    14761 tggaaatcga actggacgga atcgaagatt cgcgggatac gctaggtttc caccaggtcg
    14821 tgaccctcgg cagccatcgc cgcgtcctgg agggattctg ccaactctac ggcatcaagg
    14881 tcacgcattc cccacggttt tccaccttcc aatacggagg ccaagcatga tccgtcacgt
    14941 ggtgttctcg ctgattttgg ctgccgcttg cgcgagccta tcgctcgccg aagttgtgga
    15001 gtttacctct ccgggctttc caccctcacg gatggatgcc gagggaaggc tcacggaaga
    15061 ctggggaagc ctcaagatca tcgttgaagg cgatggggtc atggcccaag gcgggacggt
    15121 tcaagtcgtg cgactggagg aacttgttcc tgctgccgta tggtcctgtg aagcgggccc
    15181 catccgcgtc acggccaccg cctatcgcgc accggtgttt ccatcgggga tggacgtgct
    15241 gaccgttacc ttacaggaga ccgcgggcca ggatcgtcac gtaacgctga agatggtagt
    15301 caatccggcg ccgcgggtcg ggctttctac tgcccaggtc gaaaagcgga cggttctcgc
    15361 catccccctc gagacgcaac aattgctgaa gctccgcgat tggggttaca ccatcgacac
    15421 cacggcgatg ccgggttggg ctaagccaga gggtgaatgc gatcccgctt ttgctaacat
    15481 ccgcgccgga atggggggta ttcccattcg ttaccggttc cgggtaacac ggcgaagcca
    15541 agccaacgtc gtcctgggga tttgtgaaag ttactgggac cgcccgcaaa tccgtccttt
    15601 aatctgcctg gtggaggggg cccgccccat ggttgttgac cccatcgatc gttggggccg
    15661 ccaccagccg ggaacactgt tattcacggc gctcgacaat gacggagacg gctggattac
    15721 cgtaacgatc aagcccgttc ccggggcacg cgaccaaaac cccatcctca atgtgatttg
    15781 ggtcttttct ggggatcaac gtcccaactt agctcgcgtg gccaagggcg agcttaacga
    15841 gcaggcactg tattacgtgg atgtcggcgg cgtgagggac caacccatcc tggaaagcca
    15901 ggacctaagt ttccccgtag agttggcagc caatagctcc aaaaagttga ccttttacgt
    15961 cgcatgtccc ggtggttcgg tggttacacc cgaactgact gcgtggacta ctgaaagttt
    16021 gttccaggca gccaaggacg tctggaaaaa ctggtcgcaa tcagtcacgg ccagataacc
    16081 aataggtcgt ttttctgcat ccgaaagacc tgacacaggt cacgtcttgt gggcgtcggt
    16141 ggcacacact caccgatagc acacggtgtg tgggttatgt gccgcgctac gaactggaag
    16201 gattgtggcc aacattcagg ttacggcaga acttcgataa tcactttccc cagctttccg
    16261 ctgaaagcgt tttcttccgg atagtcgccg accaagccgt tgtcatcccg accaatttgc
    16321 aggccgtctt gcggcatgtt tgtaagcggt ggaatttcct tcgcttccgc taggacagcc
    16381 ttattccaca aaatttttac cacatcgttg gaccaagtta cggagagtac gcctggtccc
    16441 tgcgacaaag gttgactgcc aaccacggtt ttcaactggt tcttatggcg gacggcaaag
    16501 cagggcttgc catcctggat gtaaagcgca tatccgtgag ccgagcctcc ctgggcgaca
    16561 ataacacctg acgtacccac ggcttgaatc tcagccgtga tttttaaccc cttgcccaca
    16621 acgtaagggg cttccgttcg tggaagttga gcgccgggcg ccaacacgaa ttgggtcttg
    16681 gtggagaatg tgtctttggt ttcggctcgc caagcaccta acggcaagac attagcacgt
    16741 ttggcccact cgtcccaggc agccgcaagt cgagctacaa cctcgggata ttgagccgag
    16801 aggtcgtgca tttccgtgcg atcggcctcc atgtcataca actcccaggg ttgattctcc
    16861 ttagcgacga gcttccattt tccatcccgg accgcgcgat tcccctcgtg ctcaaaaaag
    16921 atcggttcct tccgcttgat cggttggccc ttgaagagag gaacaaggct cacgccttcc
    16981 atgggaatga ttttgtggcc ttgaaactcg gtcgggtatt gcgccccggc caactccaca
    17041 caggtggcca tgaggtcgat caaatgcccc ggttggtgtt ccagatcgcc acggcgcgtt
    17101 aggcctgccg gccaatggac aatcaatgga gtggcaatcc ccccttcatg cacaaagtgc
    17161 ttgtattcac ggaacggcgt gttggaaacg ttcgcccaat tgatgccgta cgaaatgtat
    17221 gtgtcctccg gtcccggcat gatgaagggg ccgcttaatg tgggaacccc gcggcggttc
    17281 tgggacggtc tgacatccgt gcgaatcgca tccagcgcaa tcggcggtaa cgaaggttcc
    17341 acggggcgac gccatgtccc cgttcggcca atcgcttcct ggcaggcacc gttgtcttgt
    17401 aggtagaaga caattgtgtt ttcccattga ccggtttccc gcagggcctc aaggattcgt
    17461 ccaatccccc gatccatgcg gtcgatctga gccgcataga cctccatgca acgaatctcc
    17521 cactccttat gctccacttt gtcccagtcg ccaacttggg ggctaagttc ccagttggga
    17581 gagattagcc ccatttgttt cagccgttcg aacctcgccc ggcgaatcgg ctcatacccc
    17641 ccatcgtagc gaccacgata tttggcaatg tcctcctcca aggcatgtaa cggccagtgg
    17701 gccgcagtat acgccacata catgaagaac ggactcgttc gcgcagtggc cacatgttcc
    17761 cggataaacc gcaccgcatg gtcactgatg gcatccgtgt agtagaacac ggaaggcttg
    17821 tactccgggt cggcaaacgg ggagatcatc gtgttatccc gggttaaggt gccgggatcg
    17881 aaataactcc cggcgcccgt gatggtcccg taaaagcggt cgaatccacg ttgcagcggc
    17941 caattgtctt tgggaccttc cggcttggtg gccttggtga catgccactt gccaacggcg
    18001 taattgcgat atcccgccgt gcggagaacc tccgcaatgg tcaccgtttg tcgattgaga
    18061 tcgccacgat aaccgtcata gccgcgatcc tccatcatgt ggccaacacc cgcctgatgg
    18121 gggtaaacac cggtcaaaag gcaggcccgc gtcggacagc atcgggctgt gttgtagaac
    18181 tgggtaaaac gcagcccatt tgcggccagg cggtccaggt tcggtgtttg gatttcacca
    18241 ccataacagc ccaaatccga aaaccccatg tcatcggaaa ggataatgat gatattcggt
    18301 cgcggcgcct ccgcggcgtg ccccgtggag aaacccaatc cgctggctga gaggacgagc
    18361 gacaaaacta gcgaaagcga tctcatggtt gacctcccca agtaactgaa acggccgaac
    18421 cggcaacaaa aaatcaattt ttgattgacg cactagcgac cttttctcgc ttatggttgt
    18481 cattttgcac atttccaaga tcaaaacaag tcaatcgcta gcagaaaagc gttttccgaa
    18541 ccagaataga cgttatggcg tatcgctgtg gggggctccg ggagaagctg acccatcgga
    18601 gggcctgatt taggcgtccc ggcaagtgcg tcttcgtttt ggaatatggc aaggcaaggt
    18661 cgataactgg atcgcgacga tgatgaccgg agtgttatca ctggatcatt tagtcgttgc
    18721 cgactggctg agttggcttc aaacgttgat tgccatgctt ccggtgtgga tgaagccact
    18781 ctggattgtg ctggtcggat tcctcgccgg atttttcctt ttgtgggttg tgctgcaatt
    18841 gatggcgcag ttcgccccgc gggtcacggc cattatgcga acaaccagca aggaagccac
    18901 ctcgcagccc cttttctaca tcctcttggc gatcggcgtg accatgcttc tgctgtcccc
    18961 catcatgccg tacaatacgt tcggcgagga catcaagctc cttaaggcgg aagggctcac
    19021 gctcatcaag cttttggcca tcattttggc cgtgtggact gccagcgtct cgatctcgga
    19081 ggaagtggag ggccgaacgg cgctcaccgt cctcagcaag cccgtcgccc gctggaagat
    19141 gttggtcggc aaatttctcg gcgtgatact cccagtcatt gtgatgttct tgatcttggg
    19201 cagtctcctt ctatgcgccg tgtcgtataa ggtcaagtac gatgcgaagg aagttgcgcg
    19261 gcctgatccc acagttcagg aatgccgcga tgagatgttg cagaccgtgc cgggattggg
    19321 gcttgccttt ttggaggccg tcgtcatggc ctcgattgcg gcggctattt ccactcgatt
    19381 cccattgatc cctaacctac tgatttgtgc aagtatatat gccctaggcc atctgctccc
    19441 caccgttgtg atcgaaacgg ctgatcgtct cgagtttgtg gcgtttgtgg ccaaactgtt
    19501 ggccgtcatc ttgcctgggc tcgagttctt cagtatggaa acggcaattg ccacggggcg
    19561 aagtgtttct gcctcctatt tgggactggc cgctttgtac agtcttgttt acatcagttt
    19621 ggctttgctc ctcggcctgc tgttgtttga agaccgcgac ctcgcgtagc tccagccata
    19681 tatcgccacg caccgttgca agcctgcgtc ggaaataccg ccgatttgtg gtgagatgtc
    19741 tcttcacatc atgaccccgg tgtgtgttgc caattgtcat catcgtttct gtccccgggt
    19801 ggtccggcac tgttgatacg ccttcgccat ttgcaccaat ccgcggctaa atcgtgattt
    19861 gccgcaaatc ctcggtcttt tccgctgggc acgtcgcccc atcaccatac ggcactggac
    19921 ttgcgatcat agatactggc gggctaatcc tttccagaat gtgaccccgt cgcaaggcca
    19981 caatcatgtc tcacgttacc gaaataaaaa cttttgacga acttgtggga caccggctcg
    20041 tgtggcgcaa gctgtggggc gagacgccga acgccagctt cttccaaagt tacgaatggt
    20101 ttgagaccta ctggcaacat ttcggaaaag agcagacgct tcgtgtcctc gttgtgcact
    20161 cgtgtgacga ggtcatcggc ttgttgccgc ttgtggttcg taccgagcgg acgcgcgtcg
    20221 gacccgttcg cgttctaacc tatccgcttc acgattgggg cagcttctac agccctattg
    20281 gcaagaatcc cacagcgacg ctattagctg ggctccgcta cgtcaaagaa tcctcccgcg
    20341 actgggatat cctcgatttg cgttgggtcg atcgctttgg tcaggatcgc atgcgaacgc
    20401 ccaccgctat gcgacaggta ggtttcgagc cctccgaaca ggtgtggatg caatctgcaa
    20461 tcgtcagcat tgatgaatca tgggagtcat actgggccag tcgaacgagc aaattccgga
    20521 acaacatccg ccgggcagaa aaacg
//
LOCUS       DAOSUT010000071        20506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1693_length_20506_cov_9.892279, whole genome shotgun sequence.
ACCESSION   DAOSUT010000071 DAOSUT010000000
VERSION     DAOSUT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20506
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1693_length_20506_cov_9.892279"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..646)
                     /locus_tag="PLD05_08250"
     CDS             complement(206..646)
                     /locus_tag="PLD05_08250"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOP77470.1"
                     /translation="MGDASSVDTGQSCPCCQGNVVVLACSGASNVGHLADLATRELTR
                     RGIARMGCLAGVAARLPGTLNVVQQARWIVVLDGCGTQCGTRILKEAGFENVLFLRVD
                     ALGMKRGETPVNEENVHRLTDAVCQEIGTLCGTTAQDSTDACCR"
     gene            complement(691..924)
                     /locus_tag="PLD05_08255"
     CDS             complement(691..924)
                     /locus_tag="PLD05_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOP77471.1"
                     /translation="MKLVQVLGPGCAKCQALAKNAEEAVKQLGIEAQIEKVADINMIT
                     SFGVMMTPALVIDGELKVQGKVASVEEIKRLLA"
     gene            complement(982..2355)
                     /locus_tag="PLD05_08260"
     CDS             complement(982..2355)
                     /locus_tag="PLD05_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOP77472.1"
                     /translation="MSRKDWGILTILVVVFLLAYFLNFASPRIQNAVMEAFLMLQWYA
                     RNHTLACVVPAMFIAGGIATFLSKEGVLRHLGPKARKVEAYAVASVAGTVLAVCSCSV
                     LPMFAGIYRVGAGLGPAAAFLYSGPAINVMAIFLTARVLGFDLGLARVVGAITFAVII
                     GLLMAWFFRKEESDRVKAFMALPESPKPKRRLWQNAAFLGSMIAFLVFSDWSDPSRTR
                     VEIAPNAKVTLMDGSQRTFNVEGPVSVAVLLETTTTYRFQMEETVGDFTKGMKINVEK
                     DVVKSKTQDTPPGYEFAAWVYRNRWYFAGAFLILTIAMALGWFERFELEAWLQETWSF
                     SKQIIPLLFIGVFVTGFVGALLPEKVVGALVGGNSITANLVASIIGAAWYFATLTEIP
                     ILEALIGLGMGKGPALALLLAGPALSLPSIAVIYSVWGFKKTATFVVLTVVMSSVVGM
                     IFGAFFG"
     gene            complement(2474..2809)
                     /locus_tag="PLD05_08265"
     CDS             complement(2474..2809)
                     /locus_tag="PLD05_08265"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOP77473.1"
                     /translation="MVKGIDPKTRARCDCRARVLKAMAHPSRLIMLEELAKSGPKCVC
                     ELAALVELDMSTASRHLSLMKEAGLLTDEKRGQMTYYHLRTPCVIKFLECVDGVIAEG
                     LRTQLSFLR"
     gene            complement(3137..6079)
                     /locus_tag="PLD05_08270"
     CDS             complement(3137..6079)
                     /locus_tag="PLD05_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77474.1"
                     /translation="MDGHFPELFEKQRTRGRATGEMPFRLTVGLVLAFLWPFLTPAQT
                     TPPAPRWRYAGGDVAVRELSHRLETVAEVTTPVERITIEAGAGTAVYYLLPLSPAPLI
                     TEMKAAVALKSRSPGLQLFLSVVLPRARHPQTGEPLTVLVPGGQYRVPGEWEWLTVTN
                     IGPVLEREVRTLRLQLRRDIDVREAYADGLVLNLYGGPGTTDVLVGDVQLEGFLPLNR
                     GPQAASPYPESPGQSGGIGSNHGTPFNNDRISRGTLPLRPERTQRDWLNTPLGSGKEL
                     DWSVTPRSGADILPAQHLAPPPIEMVGSVLRVKGRPFFPRALEYHGEPLDLVRQLGFN
                     TIWIREPVEERLLLEARQQGLMVILRPDPKLFGLAQSGVQRTIGSSYDGVLAWDMGTQ
                     LSPQNVEELKGLAEQIRSVDPMANRPILAEPLTQLLPVSRAVDVLLIGRNPCFSDLEL
                     TDYGTWLRSRPLLARPGTPVWVTVPTQPDPGVLSQWERWGLSADQLNRIPADQVRLLT
                     YLALTAGARGLLFRSQSSLACPDEATRERCIALELLNRELEWIDPFLAAGQFLSMVPS
                     DQREVIGALFRTDRARLLVPLWLGRRAQYVPGQSAADQVTFVIPGVPESNSAYLLLPN
                     SLEPLEGERVAGGMRVKIKEFGPTSLIVLTQDPVVLGFLMDGARTAGPRIVQLERELA
                     EMTFRQVQKQHQVLMKYGPFVPQADSWFRMAAQEMAAADKSVGAGDYPAAFRHVHRAM
                     RPVLLIRRVDWEAFALSLDNIVAWPCAVSFDTLLAHLKWTDQLSEDRARPTLLPGGDF
                     EAGGQITAMGWQPFQHAIPGIVAQAELSPSAARSGSFGLLISVRATQPQREQALVESP
                     PIWIESPPVAVQPGDWVCIEGWISIPRAITGSVDGLMIVDSLGGEVLARRMSQTVGWQ
                     PFRLYRRAGNEGQVRITLVMTGLGEVCLDDLAVTVIPTDGPSQQPPVPDVQPLVPTLT
                     QPLAGVGR"
     gene            6432..6725
                     /locus_tag="PLD05_08275"
     CDS             6432..6725
                     /locus_tag="PLD05_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOP77475.1"
                     /translation="MAQPHSLRPSDPATIPSELQELANAIQGLPEPYRAQLDPVFQRV
                     MDGIRRRRQILSLVQDALSELRMDMKYLLFDLEATRRERDEYRRQLEQLRPGE"
     gene            7538..8665
                     /gene="arsB"
                     /locus_tag="PLD05_08280"
     CDS             7538..8665
                     /gene="arsB"
                     /locus_tag="PLD05_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOP77476.1"
                     /translation="MVNDLQHEDNRKDDVATTDVGAPAPTRKRLNFFERYLSVWVALC
                     MVAGVILGKSLPDLTATLRTWEFASGSQINIPIAVLIWLMIIPMMMKVDFASIRNVGR
                     RPKGLLITLFINWVVKPFSMALWAWLFFRYVFAAWIGTDDASQYIAGTIILAAAPCTA
                     MVFVWSYLTDGDPAYTLVQVSLNDLIMLVLFAPIVRFLVAGASSLHVPFQVLFYSVVV
                     FIVIPLSVGVILRRWFVSRRGIQWFEQELLPHLAPVSMAALLVTLILIFAFQADNITN
                     KFWHVVLIAVPILVQVYFNSSLTYWLMYLFRVPHNIASPGALIGASNFFELAVATAIA
                     LFGPESGAALATVVGVLIEVPVMLSVCAFCNRTRHWFAGFQ"
     gene            complement(9018..10241)
                     /locus_tag="PLD05_08285"
     CDS             complement(9018..10241)
                     /locus_tag="PLD05_08285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP77477.1"
                     /translation="MSSFNLSNLWNFGLQPRPMFRLRMPQNRAAPVEVRPVRTWREQY
                     YFTRLPWLIYQNDPAWVPPLLIEVKEFLNPRKHPFLLHGQATQFLACQDGCPVGRILV
                     SVDPRYNAKHGTKVGCFGLFESINDVRVAHALFSAAAEWLRQRGLTHMMGPVDYSINY
                     PCGLLIEGFQYPPRIMMNHNPPYYAELCESWGFQKAKDLYAWWFVKDNENLRTWMKRV
                     ARLRQRSRITIRPFNLHDFENDMARCVEVYTATRYDQWGFIDLTPAEFRYFAKRLAQF
                     ARPELVLVAEDNGKAVGFSITVPDLNEAIRPLNGRLTSWGMPIGAVRLMQRLRHVKTA
                     RVMVLGVISGYRHRGIAELMIYETIKRGTEEANFEAAELSWTLEDNHAINNMIASVGG
                     KLYKRYRVYEKAIVD"
     gene            complement(10306..11430)
                     /locus_tag="PLD05_08290"
     CDS             complement(10306..11430)
                     /locus_tag="PLD05_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOP77478.1"
                     /translation="MSNIVVKPVETKKEQRLFLEFPWRLYHGDPNWVPPLRGNQRALV
                     GFAPHPFYERNRSQAFLAYRDGEVAGRILAIINQGHIERFQERRGFFGFFESVDDERV
                     AHGLFDAARQWLASHGLETLRGPTNPSLNYELGLLIEGFDSPPTFMMTYNPPYYQRLI
                     ESYGFRKTQDLYAYWGHVSMLPEIRAKLKPISDKIVERYNVKIRMLDKRRFLEDVELF
                     LNIYNRSLINTWGFVPMTPAEVRYMAKGLRWLMVPELALGVEVDGRMVGAVFCLPDYN
                     QRIKEINGRLFPFGFIHLLRNKHAIKRVRVISTNVVPEYQLMGFGLVLLDGLVPKALE
                     WGIEEAEFSWVLESNALSRGSLEKGGAKRTKTYRLYDYGG"
     gene            11645..13075
                     /locus_tag="PLD05_08295"
     CDS             11645..13075
                     /locus_tag="PLD05_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77479.1"
                     /translation="MIRSLQRRQFLELTVLGTAALGMRQWAWSSQAPASKLISPGCRR
                     SKVKIAKLYLAGSGLWPHPKLDVEAERQSYEQAFKEMADQFADVDFFVDELITSPKQI
                     SDLADKLAQAVGILAIHIRMGVGAMLAELYKVGRPMMIFAVPYSGHEWSGFGAVLRQA
                     EPPPMDVILSADKRDLATAVRPIRALHHLKDAKIVNVTLREPGDFHQAVKDKFGTEIK
                     HIGLEPVVALYEKVSDQDAEAEAKKWMEGAKQIVEPTPEEIFRSCKLALAFERLLDEE
                     DATVLTVDCYGTMWDRTIKLPAYPCLGFSRLNGMGLGGICESDLRCAMTHIIFQGLAG
                     KPGFISDPTVDQSTNTIILAHCMGTVKMDGVNSPDMPYKLRCVMERQEGVTPQVFVPR
                     GVRATQALLVDTNTLLYFTGEVVDAPDIERGCRTKITVRVDGDVEKLWRNWSSGLHRS
                     TCLGDLTKDLERFARLSKMRLVNELA"
     gene            complement(13552..>13983)
                     /locus_tag="PLD05_08300"
     CDS             complement(13552..>13983)
                     /locus_tag="PLD05_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77480.1"
                     /translation="YLLKFAANVPDQVREQEQMVRLRGIPRMAASKVKLVDLMPEYQA
                     DKINEVLEGPEGREILAFCTNNVEQRLEALKQSPHVDSNRVKLLEALWRTVLTLKRNA
                     DNPPTTYQGYSQVAIQQSDNLKRQVTQLADSFSQLRPAYAD"
     assembly_gap    13984..14083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14084..15738)
                     /locus_tag="PLD05_08305"
     CDS             complement(<14084..15738)
                     /locus_tag="PLD05_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOP77481.1"
                     /translation="MRVSRLSRLWVCWPLLGLLVLAVGCPQSSVDSSAFRVKTASQEP
                     STSNSNTSEGAAAPAESASTGPVTQQTVTTPPPTPEQNQQVPSPVATPNPPVSENASS
                     QPSPTAPSASHGTDQAAANAEPPSSPPPSAPSPPATDLLALVNPATHCLMGQWTREGK
                     GFRSPAQAYTTLVFPFDPPGQFRLLIVARRIEGYESLNVTLPVGGSQAMVVLDGFGRR
                     ISGLSMVSGRTADANETRFFGFSFLPNMPVPIQFFCSPNRIAVQAGSRWIIDWRGSPG
                     VLSLDSRFWKHIPPNRIAISVYSPNTVFVIERAELTPLPEGLGLPEETLVPGRPGSSL
                     AGGPGSRPPFGPPWGRFDGPPESPAESPSVQESASPPASQTASEPPPSNVPITHFHEE
                     PPEAVTQWKDSVGLIEYPLGSGTGFVVKENLIATNAHVIEGAFAEDLEISFTGAQEGR
                     YRVQKILFEDPARDLAILYVTCPQKPVTLAQNSELRTGDKVAIISNPSLGKTDIVVRN
                     AIVAGQVAARVHTKGYDFYQVNANVNPGSSGAPLFNWQGEVVGV"
     gene            16029..17324
                     /locus_tag="PLD05_08310"
     CDS             16029..17324
                     /locus_tag="PLD05_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP77482.1"
                     /translation="MKLPPEGAVWLVRLRWFVALCVFAATFLGYFVFHVLATPIPLIL
                     VGVLMLVYNGILALVVREDSPIRGLRPEDSIFAQIILDFAALTLVLYFSDFSHNPFIL
                     YYIFHIIIAGMYLRGIRPYLVAGGTTLVIGAIIWAEYVGWLPRFPLRFGGQELPPLHG
                     LQALLLFLAMASSLGLATYFTTSIRRYVDRAHAELRQKEKMLGIGQLVAGIAHQIANP
                     LDGVQNCLRRIGEAVKNDARLTEYVQMMAEALERIERTAKRVQAFAQPRGVSLEKTDV
                     NAAVEGTLAMLGTRIGNNVRVEKDLRQVPAVLGDKYTIQEVVFNLCTNALAAMPNGGT
                     LTLRTRTLGTQDDDQMNSVAIEVSDTGVGIPRANLEKIFEPFFTTRAESGGTGLGLGL
                     CRMLISEMGGRIEVRSALGQGTTFTVILNAYRDPHGDRR"
     gene            17340..18692
                     /locus_tag="PLD05_08315"
     CDS             17340..18692
                     /locus_tag="PLD05_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP77483.1"
                     /translation="MRVLIVDDEKIKRVTLADDLAAQGYEVETAADGEEALERLRDRH
                     FDVVVADVKMPRVDGLELLRYIKQGEQAPATDVIMMTAYGSIQLAVESMKLGAYDFIT
                     KPFRNEDLFPVLARLQKERGRRISSREVEGPKANDIESSVIGQSAVMTRVKKMIEICT
                     RTDANVLLCGETGVGKDLIAQVIHRNSHRAQQPFVKVGCTLFPAHLIESELYGHEKGA
                     FTGADQRRPGRFEIAQGGTLYLDDVDDIPLEQQAKLLRVIEEKVFERVGGTTPIQADV
                     RIIASTKQNLLEKTAQGTFRQDLYYRLDVLRINIPPLRERREDIPLLAEHFMKKIAGD
                     QPYKIDPAAIQVLQEHEWPGNIRELYHTLERAYLMGSGNITADVVRAEMAYFPIKDMK
                     GTAAKLMPSTFQDAINQAERELLEKALREAGGNKTAAAAALGMKPSTFRDKLAKHGLG
                     "
     gene            complement(19007..20371)
                     /locus_tag="PLD05_08320"
     CDS             complement(19007..20371)
                     /locus_tag="PLD05_08320"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOP77484.1"
                     /translation="MSDALASPAKVESPIKRVAILFSGGPAPAANAVIASAAIAFSRV
                     GVEVLGIRHGYTHLMAYDGQKPLEEGKAFIRLRHEDLELVRSQQGIIIGTARANPGKS
                     VHTYEDLKDPQKTAPLKTVYDALCSLGVDALISIGGDDTLTTAAKFQLFQKNLPAGSK
                     RIAVVHLPKTIDNDYEGIDFTFGYFTAVDMLATQVRNLLADARAAGAYFFVQVMGRKA
                     GWLAYGAGIAGEASLVVSLEDIPPEWWTKEVTVNPKTGQQVIDPATGQPQMRDVFDVR
                     ALAKRVADTVIAREKEGKGYGVVVFAEGMGEYFPLEELRKCVSEEEFAELEPDPFGHF
                     PVSQLKFTGRLARLVNQELQARGGKKKKISSLQFGYEVRCQRPTAFDIILGSQLGVGA
                     YRALVERQLDGVMVSVTGQLQLVFPSFEDLMQKGKLRAKERLIDPKEDFHQLPRYLEV
                     RIDR"
BASE COUNT         4607 a         5690 c         5558 g         4551 t
ORIGIN      
        1 tggcgattct cgacttgata gtttcaccgc gatggcacca aaagcagcac ctaccaatag
       61 cagcagtgcg ccagttaaaa gtgtttgcca cgagttgttt gggttcatga ggaacctcct
      121 cttctgtgat catgactcga cgaaaatgca atccaaacgc cgaggatgcg accacatccc
      181 cggttcgatt gcggttcaca aaaggttatc ggcaacacgc atccgtggaa tcttgtgccg
      241 tcgttccgca taatgtgcca atttcctggc aaacggcgtc ggttaatcga tgcacgtttt
      301 cttcgttgac gggcgtttct ccccgtttca tgcccaaggc atcgacacga agaaacagca
      361 cgttctcaaa accggcctcc ttcaagatac gagttccgca ctgggtacca cacccatcca
      421 ggacgacaat ccagcgagcc tgctgcacca cgttaagtgt tccgggcaag cgcgctgcca
      481 ccccagccag gcagcccatt cgggcaatgc cgcggcgggt caattccctc gtggccagat
      541 cggcgagatg gccaacattc gaggcacctg aacacgctaa caccaccaca ttgccctggc
      601 agcaggggca cgattgcccc gtatcgactg atgacgcatc acccatgggt cgaatcctta
      661 gttgttttgt gccaaaacgg gatattcatc ctaggcgagc agtcgcttaa tctcctcgac
      721 gcttgcgacc ttaccctgga ctttcagttc tccatcaatc accagcgcgg gcgtcatcat
      781 aacgccaaaa cttgtgatca tgttgatgtc ggcaaccttt tcaatttggg cctcgatacc
      841 caattgtttg acggcttctt ccgcgttctt agccagggcc tgacacttgg cacaaccggg
      901 accaaggacc tgcacgagct tcataggtta acctcctatt gcagtaactc tatacgaaga
      961 cacacgttga caacaagttt ttcacccgaa aaacgctcca aatatcattc cgaccacact
     1021 gctcatgaca accgtcagaa cgacaaatgt ggccgttttc ttaaatcccc atacgctgta
     1081 aatcacggca atactgggca aggagagggc tggaccggct agcagtaagg ccaaggcggg
     1141 gcctttgccc atgccaagac caatcagggc ctcaagaatt gggatttctg tcaaggtcgc
     1201 gaagtaccag gctgccccaa tgatagaggc cacaaggttc gcggtgatgc tattgccgcc
     1261 aaccagagcc cccaccactt tttccggcaa aagggcccct acaaaaccgg tgacaaagac
     1321 accaatgaac agcagcggga tgatttgctt ggaaaatgac cacgtctcct gcagccaggc
     1381 ctctaactca aatctttcaa accatcccag ggccatggca attgtcaaaa tcaagaaggc
     1441 ccctgcaaaa taccagcggt tacgataaac ccacgccgca aattcgtatc ccggcggggt
     1501 atcttgggtc ttcgatttta ccacatcttt ttcgacgtta attttcatcc ccttggtaaa
     1561 atcacccacc gtctcttcca tttgaaaccg gtaagttgtt gtggtttcca aaaggacagc
     1621 cacactcaca ggcccctcca cattaaaggt ccgctgggaa ccgtccatca gcgtgacttt
     1681 cgcattaggc gctatttcca cacgtgttcg acttgggtca ctccaatcgg agaagaccag
     1741 aaacgcaatc atcgaaccaa ggaacgccgc gttctgccaa agacgacgtt tcggtttcgg
     1801 cgactcaggg agtgccatga aggccttgac ccgatcgctc tcctctttac ggaagaacca
     1861 ggccatcaaa aggccaatga tcacggcgaa agttatcgct cccacaaccc gtgcaagacc
     1921 caggtcaaaa ccgaggacgc gggctgtgag aaagatggcc atgacattga tcgccggtcc
     1981 cgagtaaaga aaggctgcgg caggtcccaa cccggctccc acacggtaaa tgcccgcaaa
     2041 catgggaagc acactgcacg agcaaacggc caagaccgtt ccagctaccg aggctacggc
     2101 gtaggcctcg acttttctgg ctttgggtcc tagatgccga aggacgccct ccttggaaag
     2161 aaacgtcgca attccaccag caatgaacat cgccggcaca acacaggcaa gcgtgtggtt
     2221 tcgggcgtac cattgaagca tgagaaacgc ttccatgacg gcgttctgaa tccgcgggga
     2281 agcaaaattg aggaagtacg ccaaaagaaa aacaaccacg aggatggtaa gaattcccca
     2341 gtcttttcga ctcattgcgt caactccttg atccgttaat ttaatgcatt cttggccaaa
     2401 tatgcaagat cattgcccaa aaagaacgac cagtggaggt ggttcttgag aaaggtaccc
     2461 caaatacctc actttaccgc aaaaaactca attgcgtccg gaggccttct gcgatgacgc
     2521 cgtcaacgca ctccaagaac ttgatcacgc aaggcgtccg cagatggtag taggtcatct
     2581 ggccccgttt ctcatcagtt aggaggcccg cctctttcat gagactcaaa tgacgggagg
     2641 cggtggacat atccagttcc accagcgccg ccagctcaca gacgcatttt ggccctgact
     2701 tggccaattc ttccaacata attaggcgcg aggggtgggc catggccttg agcacccgtg
     2761 cccggcaatc gcacctcgcc cgcgttttcg gatcgattcc ctttaccaca accgagttcc
     2821 tcttttgtca gtttgacatc tcgtgattga gacaaacgcg ttcttgcatc aattcattct
     2881 tgcacatatt tgcaaatgta tcatccattg tatcgatcga caagggtaaa aggttcaaaa
     2941 attcttgcaa caacaccgaa ctggacccct gtatggttac ccaggacccc gcaggatggc
     3001 gtccgagcct gtcttatgcc gtttccaaga ccctgtgtcg ttccctgccg tccgccgagc
     3061 ctgccgatca tttcccaacg acagcctata cggcggtagc gacgttcttt tgcggttttg
     3121 aggggaaatc ttgcatttag cggccaacgc ccgccaaggg ttgcgttaac gtagggacga
     3181 gcggctggac gtcgggaacg ggaggttgtt gggaagggcc atcggttggg atgaccgtga
     3241 cggcgagatc atcaaggcat acctcgccca atcccgtcat gaccaaggtg attcgcactt
     3301 ggccctcatt tcctgcccgc cggtagaggc gaaagggttg ccaacccacg gtctggctca
     3361 ttcgtcgagc caggacctcg ccgccgaggg aatcaacgat cataaggcca tccacgctcc
     3421 cggtgatggc tcgaggaata gagatccacc cttcaatgca gacccaatca cctggctgaa
     3481 cggctaccgg tgggctttcg atccagatcg gcggcgactc gaccagggcc tgttcgcgtt
     3541 gcggttgcgt cgcccgcacc gagatgagta ggccaaagct ccccgaacgg gcagcagatg
     3601 gtgacaattc cgcctgggca acgatccccg ggatcgcgtg ttgaaacggc tgccagccca
     3661 tggccgtgat ctgcccacct gcctcgaaat caccgcctgg gagcagtgtc ggccttgccc
     3721 gatcctcgga caattggtct gtccatttca ggtgggccag tagggtatca aaactgaccg
     3781 cgcagggcca ggcaacgatg ttgtccaaag acagtgcgaa tgcttcccag tcaacccgcc
     3841 ggatgagcag gacaggccgc atcgcccgat ggacatgccg aaacgcagcc ggataatcgc
     3901 ccgcccctac cgatttgtcg gcagcggcca tctcttgggc ggccatgcga aaccacgaat
     3961 cggcctgggg aacgaatgga ccatacttca tcaggacctg atgctgcttc tgcacctgac
     4021 gaaaggtcat ctcggccaat tctcgttcca attggacaat gcgtggtccc gcggtccgtg
     4081 ccccgtccat caaaaacccc agaaccacgg ggtcctgggt caggacgatg agagaagttg
     4141 ggccaaactc cttgattttg acgcgcattc ctcctgcgac gcgttctcct tccagtggtt
     4201 ccaaactatt gggaagtagc agatacgctg aattgctttc cggcacaccc gggatgacaa
     4261 aagtcacttg atccgccgcc gactgccctg gtacatactg ggcccgccgt cccagccaga
     4321 gtggtacgag gagtcgcgca cgatcggttc ggaatagggc cccgatcact tctcgttgat
     4381 cactggggac catggaaagg aactgcccgg ctgcgagaaa cggatcaatc cattccaatt
     4441 cccgattgag taactccaag gctatacacc gttcccgcgt tgcctcatcg ggacaagcca
     4501 gggaggattg ggagcggaac aatagccccc tcgccccggc cgtcaaggcc agataggtca
     4561 acagacgcac ctggtccgca ggaattcgat tgagttggtc cgccgacagt ccccaccgct
     4621 cccactgcga caacacgccg gggtccggtt gtgtggggac ggtcacccaa acaggggttc
     4681 caggtcgggc aagcaacggt cggctgcgga gccaagtccc gtagtcggtc agttccaagt
     4741 cggaaaagca ggggtttcgg ccgatcaaaa gcacatcgac cgctcgacta acgggcagca
     4801 attgggtcaa cggttcggcc agaatcggac gattagccat gggatcgacg cttcttatct
     4861 gttcggccaa gcccttcagt tcttcgacgt tttggggtga aagttgcgtc cccatgtccc
     4921 aagcgagaac gccatcgtac gatgagccga tggtccgctg gaccccgctc tgcgccaagc
     4981 caaagagttt cggatccggt cgcagaatga ccatcaatcc ttgttgccgc gcttcaagaa
     5041 gaaggcgttc ctcgaccggc tctcggatcc agattgtgtt gaaccccagt tgccgcacga
     5101 ggtccaacgg ttccccatgg tactccaacg cccgtgggaa aaacgggcgg ccttttaccc
     5161 gcagcaccga gccgaccatt tcgatcggag gcggggccaa gtgttgggcc ggaaggatgt
     5221 ccgcgccgct acgaggggtc acgctccaat cgagctcttt accgcttccc aagggcgtgt
     5281 tcaaccaatc tcgctgcgtc cgctctggcc taagtggcaa agtaccgcgg gagatccggt
     5341 cattgttaaa cggcgtccca tggtttgacc caattccccc agattggccc ggcgactcgg
     5401 gatagggcga cgccgcctgt gggccccggt taagcggcaa gaagccttcc agttgcacat
     5461 cgccaaccaa gacgtcggtg gtccctggcc caccatagag gttaagtacc aaaccatcag
     5521 cgtaggcctc gcggacatcg atgtcacgcc gcagttgaag acggagtgtg cgaacttccc
     5581 tctctaagac cggtccaatg ttcgtaaccg ttagccattc ccattccccg ggtacgcgat
     5641 attggccacc gggaaccaac accgtgagag gttcacctgt ttggggatgt cgggcacgcg
     5701 gtagaacgac cgacaaaaat agctgcaaac cgggggaacg gctcttcaac gccacagccg
     5761 ccttcatttc ggtgatgagc ggcgcagggg acaaaggcaa gaggtagtac accgccgtac
     5821 cagctccggc ctcgatggtg attctttcca cgggtgtggt aacctcggca acagtctcta
     5881 gccgatgaga gagttcccga accgcaacat caccaccggc atacctccac cggggcgccg
     5941 gaggagtggt ttgtgctggc gtcaagaacg gccaaagaaa ggcaaggacc aatcctacgg
     6001 taagccggaa tggcatctcc cccgtcgccc gcccccgggt gcgctgtttt tcgaagagtt
     6061 ctgggaaatg accatccatg gtaccgtctt ttctggaagg gacggtctcc gatttcccca
     6121 ccggcagatc ggttactcca gcgagactgc gtcgcctagg taatcagggc aacaaaagca
     6181 agatgttgac tttaggcaac agatgcgcgg aaacgtccct aaatcgtaaa ttacgttttt
     6241 atcacgattt gcggtatttt gccagagcaa atgttgtgct ggggcaacac gctttgtttt
     6301 acgcacaaag cgagtcatag ccttaagtca ttttcagtaa ataacttaag ggaaaagtgg
     6361 ctcgttggcg tggcggcggc gcggcggatg catttctctc cttctgcaca agcccaaggg
     6421 agagacgcac catggcacag ccgcattcgc ttcgacccag tgacccagca acgattccct
     6481 cggaactcca ggagttggcc aacgccattc aagggttgcc tgagccttat cgtgcccaac
     6541 ttgatccggt cttccagcgc gtgatggatg ggataaggcg tcggcgccaa atcctgtcac
     6601 ttgttcagga cgcgctatcc gagctgcgga tggatatgaa gtacctcctc tttgaccttg
     6661 aggccacccg ccgggaacgt gacgaatacc gtcgtcaact tgagcaactc cgccccggcg
     6721 agtaacacct ctccgagtgt aacatacgcg tcacctcctt cttcggcagc ggtagtgcag
     6781 gcccaactgt actaccgcct tgtctttgtg gtggggcaat tatcatcgtg ctcagcgaaa
     6841 ggggcggtat aacttttggg cagacctttt gaagtgccaa atctcgtgca cttgccgaca
     6901 atgttcgcca tcgcgctttg gcatcgattt cacaagagat agccattgcc gtcaaaacag
     6961 tacgcgacac gaaggactgt tcacgccgtc tcaaggatga acgggatgtt tctctcacag
     7021 aagacatgaa aagtggccat ccctaattct cccttgttca cttagtttgt tcggcgactt
     7081 ccacccttgc ccgcaatcct cggctgtgct aaaataaatt gcatgtttgg ccaactggcc
     7141 aaatgtaagg cgatcgcgaa catcaccgat ttacggcccc gcgtctcggc tgggtcatac
     7201 gtggctacac gggtcttccg tggtttcggg ccgccggagg cagtgtcctg tgcgtgcacg
     7261 gttctcagaa cgtacaggat ggatatgagc gacattccac gtcgaggcga cgcatggtca
     7321 ggcctgactc gagtatcagg tggaagcctc gggctgcggg tcgatgagga gtctgctgcc
     7381 caaaggactg ccttgctgtg gtctgtcagg tcgttttaga gaccgtggtc gctccaaaac
     7441 ggaaccaaca gcgctggcat tttttgattt tcttgcggat ttggtaagct caaacgcata
     7501 gatcttaaac atcggcaatc gagttggata ggtagccatg gtaaacgatc tacagcatga
     7561 ggataacaga aaagacgacg tagcaacgac ggacgttggt gcccctgcgc cgacccggaa
     7621 acgtctcaat ttctttgaac gctatttgag tgtgtgggta gccctatgca tggtcgccgg
     7681 ggtcatcctg ggaaaatcat tgcccgactt gacggcaaca ctgcgaacct gggagttcgc
     7741 ttcgggcagc caaatcaaca ttccgattgc cgtgctgatt tggctcatga ttattcccat
     7801 gatgatgaag gtcgatttcg cctcgattcg caatgtaggt cgtcggccga agggactttt
     7861 gatcacgctt ttcatcaatt gggtggtaaa accgttttcg atggcccttt gggcgtggtt
     7921 gtttttccgt tatgtgtttg ctgcctggat cgggacggat gatgccagcc aatacattgc
     7981 cgggaccatt attttggccg cggctccctg cacggcgatg gtctttgtct ggagctattt
     8041 gacggacggc gatccggcct acacgctggt gcaagtgtcc ctcaacgacc tgattatgct
     8101 ggtgctcttt gccccgattg tgcgattcct ggtggcaggc gcttcgtcac tccatgttcc
     8161 atttcaggtg ctcttttatt ctgtcgtcgt gttcattgtt attcccctaa gcgtcggtgt
     8221 cattttgagg cgctggtttg tctcacgccg tgggatccag tggttcgagc aagaattgct
     8281 gccacatttg gcgcccgtca gcatggccgc tttgcttgtt acgcttatcc tcatctttgc
     8341 ctttcaggct gataacatta ccaataagtt ttggcacgtt gtgcttattg ctgtgcccat
     8401 cttggttcag gtgtatttca attcgtcgct gacatattgg ctcatgtatc tcttccgggt
     8461 accacacaat atcgcgtctc ccggcgcctt gataggagcc agcaactttt tcgagttggc
     8521 tgtggcaacg gcgatagccc tttttggccc ggaatcagga gcggccttgg ctacggtggt
     8581 cggcgtcttg atcgaggtcc ccgtgatgct ttcggtgtgc gctttttgta atcggacgcg
     8641 gcactggttt gcaggttttc aatagacctt tcatcgaaac gcgggaggtg tctaaagcag
     8701 cccggcagcc gctcaacacg ggaaagtggt ttagagccta tcccacaatc tcctctcgcc
     8761 cgttcggcgg gagaagggtc cggcgtgaag gtgcctcgta tcactgccgg tcttctcaat
     8821 cgattcggac gttgacttgc cctcacccca accctctccc ggagggagag gggcagtttt
     8881 taggataggc tcaaaaacaa gaaggcagtt aacggacaga gctcttgatt gttccggctt
     8941 cgacaggtgt ccgtctcatc agtcgtccag aggtggacca agccatcgat gaggacgctg
     9001 acggtggctt atatctttca atcgacgatg gctttttcgt acacgcgata ccgtttgtag
     9061 agctttccac ccacagaagc aatcatgtta ttaatggcgt gattatcttc gagggtccaa
     9121 cttagttccg cggcctcgaa gttggcttct tccgtgccgc gtttgatcgt ttcgtagatc
     9181 attagttcag caatgccgcg atgccgatag ccagagatga cgcccaggac catgacgcgg
     9241 gcagttttaa cgtggcgcaa acgctgcata agcctaacgg cgccgatcgg cattccccag
     9301 gaggttaggc gtccattgag cgggcgaatg gcctcattga ggtctggcac ggtgattgaa
     9361 aagccgacgg ccttgccgtt gtcctctgcc acaagcacca attccggtcg agcgaactgc
     9421 gccaatcgtt tggcaaaata gcggaattcg gcgggcgtaa ggtcaataaa gccccattga
     9481 tcatagcgcg ttgcggtgta aacttccacg caacgcgcca tatcattttc aaagtcatgc
     9541 agattgaagg ggcgaatggt gatgcgtgaa cgttgccgaa gacgggccac ccgcttcatc
     9601 cacgtgcgaa ggttttcgtt gtccttcaca aaccaccagg catagaggtc cttggccttc
     9661 tgaaaccccc aagactcgca tagctcggcg taatacggcg gattatgatt catcatgatc
     9721 cgcggcggat attgaaatcc ttcaatgaga agtccacagg gataattgat cgagtagtcc
     9781 acgggcccca tcatatgggt caaaccccgt tgccggagcc attcggcggc agccgaaaag
     9841 agggcatgcg ccaccctcac gtcgttgatc gattcaaata gtccaaaaca gcccaccttc
     9901 gtcccgtgtt tcgcattgta acgaggatca acactcacaa ggatgcgtcc cacgggacac
     9961 ccgtcctgac aggccagaaa ttgggtcgcc tgaccatgga gaagaaacgg atgcttgcgc
    10021 gggttgagga actctttgac ctcgataagg agcggcggaa cccaggccgg gtcgttctga
    10081 tagatcagcc acggcagtcg cgtgaaatag tattgctcgc gccaggtacg gacaggtcgc
    10141 acctccaccg gggctgctcg attctgtggc atccgcagcc ggaacatggg ccgtggttgc
    10201 aggccgaaat tccagagatt cgaaagattg aacgatgaca tcgttatatg aatccgggcc
    10261 ggattgtcag tcaatcacat atgccttgag ccatcccgca ggtgctcatc ccccgtagtc
    10321 gtacaaccga tacgtttttg ttcgctttgc cccgcctttt tcgaggctcc ctcgggaaag
    10381 agcatttgac tccaagaccc aagagaattc cgcttcttcg attccccatt ccaaagcttt
    10441 tggaaccaat ccgtccaaaa gaaccagccc aaatcccatc aattggtatt cgggcacaac
    10501 gttagtgctg atgacgcgaa cacgtttaat ggcgtgcttg ttcctcaaaa ggtggataaa
    10561 accaaaagga aacaagcgac cattgatttc tttgatccgc tggttgtagt cgggcaggca
    10621 gaagacggca cccaccatgc ggccatcgac ttctacgccc agcgccagtt caggcaccat
    10681 caaccagcgg agtcccttcg ccatgtaacg gacctccgca ggagtcatgg ggacaaaccc
    10741 ccatgtgttg atcagcgatc ggttatagat gttgagaaat agttcaacgt cttccaggaa
    10801 tctccttttg tccaacatgc ggattttgac gttatatcgc tccacaatct tgtcgctgat
    10861 gggcttcagt tttgcccgaa tctctgggag catactgaca tgcccccagt aggcgtaaag
    10921 gtcttgcgtc tttcgaaaac cgtaggactc gatcagccgc tgatagtagg gcggattgta
    10981 agtcatcata aaggtcggcg gcgagtcaaa gccttcgatc agcaaaccca gttcgtagtt
    11041 gagactggga ttggtcggtc cccgtagggt ttccaggccg tgactggcga gccattgccg
    11101 cgcggcatcg aacaggccgt gtgccacccg ttcatcgtcc acagactcaa agaacccaaa
    11161 aaaacctcgc cgttcctgga atcgctcaat atgcccctgg ttgataatcg ccagaatacg
    11221 cccggcaacc tcgccatcgc ggtaggccag aaaggcctga gaacgattcc gttcatagaa
    11281 agggtgcggc gcaaacccca ccagcgcccg ctggttaccc ctcaatggcg gtacccaatt
    11341 cgggtcccca tggtagagcc gccagggaaa ttctagaaac aaccgctgtt ctttttttgt
    11401 ctcgacgggc ttgacaacaa tgtttgacat aaatgggcaa ctttccaaaa tatccgacaa
    11461 gcactcaccg cggattgtat cgcctgattt cgcttggggt caattgaaaa atcgtaatcc
    11521 cttgcggcct agcttgacaa ctcccacttg gcaaatcatg atgacgcacg ccgggatcag
    11581 ggttcggtca gagtgaattg gtacttcgaa agaaggtttc attgggagag tcacatagtc
    11641 acatatgatt cgttcacttc agcggcgaca attcttggaa ctgacagtcc ttggaacagc
    11701 ggccttgggg atgcgccaat gggcgtggtc atcgcaggcc ccggcgagca agcttattag
    11761 tcctggttgc cgccgatcca aggttaagat cgccaaatta tatttggcgg gctcagggct
    11821 ttggccgcat cccaagctgg atgtcgaagc agaaaggcaa tcgtacgagc aagcctttaa
    11881 ggaaatggcg gatcaattcg ccgatgtcga tttcttcgtc gatgaactca tcacatcacc
    11941 caaacaaatc tcggacttgg ccgacaaact cgctcaggcg gtcggcattc tggccatcca
    12001 tattaggatg ggcgtcgggg ccatgttggc tgaactttac aaagtcggtc gccccatgat
    12061 gatctttgcc gttccctact ccggccatga gtggtctggt tttggtgcag tcctccgcca
    12121 ggccgaaccg ccccccatgg acgttatcct aagcgcagac aaacgcgatc tggccacggc
    12181 ggttcgcccc attcgggcac ttcatcacct caaagatgcc aaaatcgtta acgtcacact
    12241 ccgtgagcca ggggatttcc atcaagccgt caaggataaa ttcggcacgg aaattaaaca
    12301 catcgggttg gaaccggtgg tggcgctcta cgaaaaagtt agtgatcagg atgccgaggc
    12361 tgaagccaag aagtggatgg aaggggccaa acagatcgtg gaaccgaccc cggaggagat
    12421 tttccgctcc tgcaaactgg ccctcgcttt tgaacggctc cttgatgaag aagacgctac
    12481 cgtgctgacg gtcgattgct acggcaccat gtgggatcgg acgattaagc tgccggctta
    12541 tccctgcttg ggattttctc gactcaacgg catgggattg gggggaatct gtgagtctga
    12601 tctccgctgc gccatgactc acatcatctt ccagggattg gccggaaagc cgggatttat
    12661 cagcgatcct accgtagatc aatcgacaaa caccatcatt ttggcccatt gcatgggcac
    12721 cgtcaaaatg gatggtgtta attccccgga catgccgtac aaactccgct gcgtgatgga
    12781 gaggcaagag ggcgtgactc ctcaagtgtt tgtgcctcgt ggtgtgcggg cgacgcaagc
    12841 cttattagtg gatacgaata cccttctcta cttcaccggt gaggttgtcg atgccccgga
    12901 catcgaacgt ggatgccgta ccaaaatcac ggttcgcgtc gatggtgatg tggaaaaact
    12961 gtggcggaat tggagctccg gcctgcatcg ctcgacgtgc ctgggcgatt tgaccaagga
    13021 tctcgagcga tttgcccgac tttccaagat gcgcctggtc aacgaactgg cctaaggcac
    13081 gcagaaggtt caaagctcca tcaatcgaaa ttgtcaggcc gaaattgacg aaaatggctc
    13141 cgccagtcgg gtgacctgat ggtttgggcg tttccgattg aagaaagggg tacttcgtac
    13201 ctctggtgca tagtgagttg ccaggattgc gttgaaacct ccatacgatt tgaacaagcg
    13261 gttcgggcac tgtaatccgg ttaccaacga gctgacaggt tctcgttgag aatatggtgg
    13321 cgggggcggt gagcttattc aggaaggtaa tccaggatga accgattgta caaaaagccc
    13381 ccattgacag cgaataatgc tgttcataga tgataggaaa attgttcact gagagccgtg
    13441 cctgcacctt ggatcggtcg aagctgcttc gagaaagaat taggcgtctg tgacgtccaa
    13501 agtcggccaa tgtttctcgt cagtgatggt acgcggtcgc ctatcccatc ctcaatcggc
    13561 ataggcaggg cgaagttgag agaatgaatc tgccaattgg gtcacctgcc ttttcaggtt
    13621 atccgattgc tgaatcgcca cctgggaata gccctgatac gtcgttggcg gattgtcggc
    13681 attgcgtttg agggtgagca cggttcgcca tagcgcttcc aggagtttca cgcggttgct
    13741 atcgacatga gggctttgtt tcaaggcttc caatcgttgc tctacgttat tggtgcaaaa
    13801 agccaagatt tctcgacctt ccggaccctc taggacctcg ttgattttat cggcctggta
    13861 ctcaggcatg agatcgacca gcttgacctt gctggcggcc atccgcggaa tcccccgcaa
    13921 gcgcaccatc tgctcttgct cgcggacttg atcgggcacg tttgcggcga acttgagcaa
    13981 atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacaccaa cgacttcacc
    14101 ttgccaattg aatagtggcg ccccactgct gcccgggttg acgttggcat tgacctggta
    14161 aaagtcgtag cccttggtat gaactcgcgc ggcgacctgg ccagcgacga tagcattacg
    14221 aacaacgatg tccgtcttgc ccaaggaagg attgctaata attgcaactt tgtcgcccgt
    14281 tcgcagctcg ctgttttgtg ccaaggtgac cggcttttgc ggacaagtca cataaaggat
    14341 cgccaagtcc ctcgccgggt cttcaaaaag gattttctgc acgcgataac gtccttcctg
    14401 cgcaccagtg aatgaaatct ccagatcttc ggcaaacgct ccttcgatga cgtgggcgtt
    14461 tgtcgcaatt agattctctt taacgacgaa tcccgtaccc gacccgagcg gatactcaat
    14521 taagccgaca gagtccttcc attgagtcac cgcttctggc ggttcttcgt ggaagtgcgt
    14581 aattgggaca ttggacggtg ggggttcgct agccgtttgc gacgctggtg gggaggctga
    14641 ctcttggact gaaggggatt ctgcagggga ttctggaggc ccatcgaaac ggccccatgg
    14701 agggccaaac ggtggacggg agccggggcc tccggcaagt gacgaaccag gtcgccccgg
    14761 caccaacgtt tcctcgggta gccctaagcc ctctggaagc ggcgtaagtt cagcacgctc
    14821 gatcacgaac actgtattcg ggctataaac actaatggca atacggttgg ggggaatgtg
    14881 tttccaaaag cgactatcca aacttaagac ccccggactc ccccgccagt cgatgatcca
    14941 acgacttccg gcttgcacag caatgcggtt cggagaacag aagaactgaa tgggcaccgg
    15001 catattgggc aggaaggaaa agccaaagaa ccgcgtctca ttggcatcag ctgttcttcc
    15061 cgacaccatc gacaggccgc tgatccttct cccgaaaccg tccaggacga ccatcgcctg
    15121 tgacccacct acaggaagcg tgacattgag cgattcgtag ccctcgatcc gccgtgccac
    15181 aattaacaga cggaactgac ctggcgggtc aaaaggaaaa accaacgtcg tgtaagcctg
    15241 ggccggcgaa cgaaaccctt tgccctctcg cgtccactga cccatcaaac agtgtgttgc
    15301 cggattgaca agtgccaaca aatctgtggc tggagggcta ggggccgatg gtggtggcga
    15361 tgacggaggt tccgcatttg cggccgcctg atcggtccca tggcttgctg aaggcgctgt
    15421 cggactgggt tggctggagg cgttctctga aaccggtgga ttcggtgtgg ctacgggact
    15481 gggaacctgt tggttttgtt cgggagttgg cgggggggtt gtcacagtct gctgtgtcac
    15541 gggaccagtg ctcgcagatt ctgccggagc cgctgccccc tcggaagtgt tggaattaga
    15601 cgttgatggt tcctgagaag cggtctttac ccgaaacgcc gacgaatcaa cactcgattg
    15661 gggacatccg actgccaaca caagaagccc taacaggggc caacaaaccc agagacgaga
    15721 caatctgctg actctcatgg tcaactcctg ccctggaaga cctcttgatt ggattagggt
    15781 attcgatgat cgaattctac aattctgcca aagcaaatgc aacgcgctac ggggaaaagc
    15841 ccgcgaagtg accaaaacac cagtgtccgc aatttgcgcg agaaggggta gccttggacc
    15901 ggcggacgcg cgctccgtgc cctgcggcaa cctttgtggc tgaaggcaat tttgccggat
    15961 tgccgagcct tgccggaacg tccttccctg cctagagtga aaaaaagtat caataggaat
    16021 tctcaacgat gaaacttcca cccgagggtg ccgtttggct tgtacgactg cggtggttcg
    16081 tcgcattgtg cgtctttgcg gcaaccttct tggggtattt tgtttttcac gtgttggcca
    16141 cgcccatacc cctgattctc gtgggcgttc tgatgctggt ttacaatggg attctggccc
    16201 ttgtcgtgcg agaagacagc cctattcgag gcctacggcc tgaggacagc attttcgctc
    16261 agatcatttt ggattttgct gcgttaacgc ttgtccttta tttctccgat ttttcgcata
    16321 atccgttcat tctttactac attttccata tcattatcgc cggcatgtac ttaagaggga
    16381 ttcgccccta cctcgttgct gggggaacaa ccttggtcat cggggccatc atctgggcgg
    16441 aatacgtggg ctggctgccg cgattcccac ttcgatttgg tgggcaggaa ttgcccccac
    16501 tgcacggttt gcaggccctt ttgttgttct tagccatggc cagcagtttg ggtttggcaa
    16561 catactttac cacatcgatc cgtcggtatg tggatcgtgc ccatgctgag ttgcggcaga
    16621 aggagaagat gttgggcatt gggcagcttg tggccgggat cgctcatcag atagcaaacc
    16681 cgcttgacgg tgttcaaaac tgcttgcgtc gtattgggga agccgtcaag aacgatgcac
    16741 ggttgacaga atacgtccag atgatggcgg aggccctgga aaggatcgaa cggacggcaa
    16801 agcgggttca agccttcgcc cagccccggg gtgtctcttt ggagaaaacg gacgttaacg
    16861 cggccgtgga agggaccctg gctatgctgg ggacgcgaat cggtaacaat gttcgtgtag
    16921 agaaagattt gcgccaggtc ccggcggttt tgggagataa atacaccatc caggaggttg
    16981 tcttcaatct atgcacgaac gccttggcgg ccatgccaaa tgggggaacg ttgacccttc
    17041 gcacgcggac ccttggaacc caagatgacg accagatgaa ctccgtggcc atcgaggtca
    17101 gtgatacggg tgtggggatt ccgcgggcca atttggaaaa gatcttcgag ccgttcttta
    17161 cgacgcgggc agagtcgggg gggactggtc tgggattagg cctttgccgc atgttaattt
    17221 cggaaatggg gggacgtatc gaggttcgga gtgccttggg gcaggggaca acgtttacgg
    17281 ttatcttgaa cgcttatcgc gacccacacg gagatcgtcg ataagatcgg ataaactcta
    17341 tgcgagtgtt aatcgttgat gatgagaaaa tcaaacgggt gaccttggcg gacgatcttg
    17401 ccgcccaggg ttacgaagta gaaactgccg ccgacggtga ggaggctctg gaaaggctcc
    17461 gcgaccgaca ttttgacgtc gtggtcgcgg atgtgaaaat gccgcgggtg gacggtttgg
    17521 agctgctccg ctacatcaaa caaggcgagc aggcccctgc cactgacgtg attatgatga
    17581 ccgcctacgg gagcattcaa ttggccgttg agtcgatgaa attgggggct tacgacttta
    17641 tcacaaagcc gtttcgcaac gaagacctgt ttccggtgct tgcccgtctt caaaaagagc
    17701 gcggacggcg aatcagttcc cgcgaagttg aggggccaaa ggccaacgac attgaaagct
    17761 ccgtcattgg gcaatcggcc gtcatgaccc gcgtcaagaa aatgatcgaa atctgcacgc
    17821 ggacagacgc caatgttctc ctgtgtggag agaccggcgt cggcaaggat ttgattgccc
    17881 aggtcattca tcgcaattct cacagggctc aacaaccgtt cgtcaaggtg ggatgtacac
    17941 tctttccggc ccacctcatc gaaagcgaac tctacgggca cgagaaaggg gcctttacag
    18001 gtgcggatca gcggcgtcct gggcgattcg aaattgccca gggtggaacg ctctatctgg
    18061 acgatgtgga cgatattccc cttgaacagc aggccaaact cctgcgcgtc atcgaggaga
    18121 aagtttttga gcgggtcgga gggacgacgc caatccaagc cgatgtgcgg atcatcgcct
    18181 cgacaaaaca gaatctcctg gaaaagacag cccaggggac gtttcggcag gacctctact
    18241 atcgccttga tgtcctccgc atcaatatcc caccgcttcg cgaacgacgt gaggacattc
    18301 ctctgctggc cgagcatttc atgaaaaaga tcgcgggtga ccagccttat aaaatcgatc
    18361 cggccgctat ccaggtcctc caagagcacg agtggccggg gaatattcgc gagctctatc
    18421 acaccctgga acgagcctat ttgatgggaa gcggtaacat caccgccgat gtcgtccgtg
    18481 ccgaaatggc ctatttcccg atcaaggaca tgaaaggcac agcagcgaaa ctaatgcctt
    18541 ccacgttcca ggatgccatc aatcaggccg aacgggaatt gttggaaaag gcacttcgcg
    18601 aggccggtgg caataagacg gcggctgcgg cagcactggg gatgaagccc tctaccttcc
    18661 gcgacaaact tgccaaacac ggtctcggct gaaagatcgt cttcgcagca cgacccgtgg
    18721 tcgcgacagg acggcgatga aaaaagacac cccgccagtc cgcggggtgt ctggctttcc
    18781 tctgaaaatc cctgcccgag gccgaatatc aaacctccgc gtcagttgat ccttccacca
    18841 tcagccttgt ggaagatcac tcttcgcacc tccgacgatg gtagggacgc cgtccccggc
    18901 gtcttaccca gggagacatg cgacccgagc gtccgccgta tttttccttc gcggacgcgc
    18961 gggttgaccc aaccacgacg aagcgtggcc ctccctctcc cggccatcaa cgatcgatgc
    19021 ggacttccag gtatcgcggc aattgatgga aatcctcttt cgggtcgatg agccgttctt
    19081 tcgcgcggag tttgcccttc tgcattaagt cttcgaacga cggaaagaca agttgcaact
    19141 ggccggtaac ggaaaccata accccgtcca actgacgttc gaccaaggca cgataggctc
    19201 ccactcccaa ttgactgccc aggataatgt cgaatgccgt gggacgctgg caacggacct
    19261 cgtaaccgaa ctgcagagaa ctaatcttct ttttctttcc tcctcgggct tgcaattcct
    19321 gattgaccag gcgggccaat cgcccagtaa atttgagctg ggaaacaggg aagtgtccga
    19381 agggatcggg ctccagctcg gcaaattcct cttcggacac acatttccgc aattcttcca
    19441 gggggaaata ctcgcccata ccttcagcga acaccaccac gccgtagcct ttgccttctt
    19501 tctcgcgggc gatcacggtg tcggccaccc gtttggccaa ggcccgcacg tcgaagacat
    19561 cacgcatctg gggctgtccc gtggcggggt caatcacctg ctgaccggtc ttggggttca
    19621 cggtaacctc tttcgtccac cattccggtg ggatgtcctc taggctgacg accaatgagg
    19681 cttcccccgc aatgcctgcg ccataggcca gccaacctgc cttgcgaccc atgacctgga
    19741 caaagaaata ggccccggct gcccgggcgt cggccaagag gttcctcacc tgcgtggcca
    19801 acatatcaac cgcggtgaag taaccgaagg tgaagtcaat cccttcgtaa tcgttatcga
    19861 tggtcttggg taagtggacc acggcgatcc gctttgatcc ggcgggaagg ttcttctgga
    19921 acaattggaa cttggccgcc gttgttaatg tgtcgtcacc accgatggaa atcagggcat
    19981 caacgcccag cgaacacagg gcatcataca ccgtcttcag tggggccgtc ttctgcgggt
    20041 ccttcaagtc ctcataagta tggacgctct taccggggtt ggcacgggcc gtaccaatga
    20101 tgatcccttg ctggctgcgg acaagttcca ggtcttcatg ccgcagtcgg atgaaggcct
    20161 ttccttcctc gagaggcttt tggccatcgt aggccatcaa atgggtgtag ccgtggcgaa
    20221 ttccaaggac ctccacgcca acgcgggaaa acgcgatggc cgcgctggcg atcacggcat
    20281 tggccgccgg agccggacca ccagaaaaca agattgcaac ccgcttgata gggctttcaa
    20341 ccttcgccgg acttgcaagt gcgtcactca ttgttcaacc atcctctctt gtgcttattg
    20401 tgctatatgc gaccacatta caagcttcac cagattaaca tcgtagagcc tatcccataa
    20461 tctcccctcg cccgtttggc gggagagggg tcgggggtga gggtgg
//
LOCUS       DAOSUT010000072        20496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1694_length_20496_cov_9.003571, whole genome shotgun sequence.
ACCESSION   DAOSUT010000072 DAOSUT010000000
VERSION     DAOSUT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20496
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1694_length_20496_cov_9.003571"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1338)
                     /locus_tag="PLD05_08325"
     CDS             complement(229..1338)
                     /locus_tag="PLD05_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOP77485.1"
                     /translation="MAAKRNRRNFLEHFVRGEILDDSPSLPSAESSQSHRPTVSKDRP
                     QGGYHLRLSRPAMGSEFQVVLNLGQYEHAVEAALEALDLLEPLENMMSFFRPAGDLGR
                     INALAATVEVPVPDALWDLLVWCRALWEETEGAFDVTAAALWQTWGFARREGRLPSES
                     EIAQARQACGWHNVQLNERDHTIRFLQSGVGLNLGSVGKGYALDRMAERLLSNGIADF
                     VLHGGLSSVLARGRSWEIDAATGQASPGWPIGLAHPLKKGAQVGRVCICDRALGTSGT
                     VRQFFYHKGKRYGHIIDPRTGYPAENALSVTVFAPTAQEADALGTAFFLLGPEKAESY
                     CRNHPEVGGIFIISPDRLQATIESVGNSPEVLEPS"
     gene            complement(1399..2115)
                     /locus_tag="PLD05_08330"
     CDS             complement(1399..2115)
                     /locus_tag="PLD05_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77486.1"
                     /translation="MTYVLLANGWTNPSVWLVLAAIILIWFSLSRAYRRRPAPRDQES
                     EFDNPEERLRSSSLGRALSSSLEQWEVRMHELTRELEGRINSKLGLLEQLIQEADRAA
                     ARLESALRTAESIRKTQAAGMLPASAHPAIEINPPAGTAEKDQTAGNVNPHIPQPQVT
                     QANLQDGITAEAPASVSSSPSTGELPDKGHPRRSVEEIYTLADYGYSPEEIAQRTGTP
                     IGEVQLILSLRKSRNSAEAN"
     gene            complement(2112..3347)
                     /gene="larC"
                     /locus_tag="PLD05_08335"
     CDS             complement(2112..3347)
                     /gene="larC"
                     /locus_tag="PLD05_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOP77487.1"
                     /translation="MTIAYFDCFSGISGDMVLGALVDAGLKLEDLQHVVSTLGIPNCR
                     VNAQTVRKGGFRATQVVVEAPEEHHHRHLHHILEMVERADLTPRQKEWAEKVFVRLAE
                     AEARVHGIPIEKVHFHEVGAADSIVDVVGSVVGLDLLGIQEVYASPVPTGAGTVKIAH
                     GEVAVPAPATAELLRGVPLLPCDVMMEMTTPTGAAILTTFAKRFGPLPAMQIEQIGYG
                     AGSRDIPGRANVLRLFVGKSLVPQGAMPGVETDRVWMVQTNWDDASGEVIGYVVEQLW
                     KLDVLDVFVTPIQMKKNRPAVQLTILCPGHLIPQVEQVLFRETTTLGIRFWPVDRHIL
                     ARRPHVVQTPYGPVQGKVSFLPDGEARFSPEFEACRKLAAEKNLPLRVVYEAAEGAFR
                     ENSANHGQTAETPPKEKTP"
     gene            complement(3821..4939)
                     /gene="tcmP"
                     /locus_tag="PLD05_08340"
     CDS             complement(3821..4939)
                     /gene="tcmP"
                     /locus_tag="PLD05_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOP77488.1"
                     /translation="MDPCPSYWLEYSNLQHTKHQLIREYLNGWFPKLGYSHSRVVYVD
                     THAGRGRHLQGQLGSPLVALQTLLQHKDRPRLLERCKVVFFFIERDETNFCELRSEIK
                     QLGNLPAKIVVNTSCKDCFKVLHDVVTSLRHAGQEMAPAFVFVDPYGFKIPGRVLREL
                     MEFPRVELFVNIIWRELDMAIRQERLRTKRSGMADTLDSIFDGSEWKDRVSSDDFDER
                     ANQAINLLREKIGAKWVTHIRMLGDNDVTRYLLMHLTNHDAGRDLMKACMWKVCPDGG
                     FYVRKTDDPAQQFLITPEPDLGPLAEWLRCRLSAGPRRWKELIAEVREQVWLPTHLNK
                     VIRELRRKKELVADDYRGRFDPINNPRLSLRVGEARGR"
     gene            complement(4948..5697)
                     /locus_tag="PLD05_08345"
     CDS             complement(4948..5697)
                     /locus_tag="PLD05_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOP77489.1"
                     /translation="MGANTTISWTKRTWNPWRGCTKISPGCKNCYMFAAQLRYGNEPH
                     TVVRTRTWNDPLRWQRQAAYQGKWEFVFTCSWSDWFHEAADEWRGEAWQIIRRCPNLI
                     FQILTKRPERIADHLPDDWGEGYANVWLGTSIELNEYVWRADVLRKIPAVIRFISAEP
                     LLGPLTDLDLEGIHWLIVGGESGARYRPMKLAWVRELRDRALAQEVAFFFKQSAGRRP
                     ETGTLLDGRHWRQYPRRVSSVPVVSVGKGPR"
     gene            6514..>6764
                     /locus_tag="PLD05_08350"
     CDS             6514..>6764
                     /locus_tag="PLD05_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOP77490.1"
                     /translation="MKMTPRYLIAMLLLLPWLGCSRPERGAEPQKQSSSSGASAEQTL
                     PAAEIRVGDQTDLARIIEENRGKVVLVDFWATWCPPCMEL"
     assembly_gap    6765..6864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7071..7874
                     /gene="panB"
                     /locus_tag="PLD05_08355"
     CDS             7071..7874
                     /gene="panB"
                     /locus_tag="PLD05_08355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOP77491.1"
                     /translation="MSAESAPLTVPKFRALKRSGRKIVMVTAYDYPSAALADLVGVDG
                     ILVGDSLGMVVQGKATTLSVTLRQMVYHAEMVARATRHALVVVDLPFLSYQTGVRDAL
                     KNAGRILKETLAQAVKLEGGRERAELIAALTGAGIPVLAHLGLRPQAIRASGGYRVER
                     DRDRLLADAEAVQQAGAFAVILECIPASVAEEITGRVDIPTIGIGAGPGCDGQILVFH
                     DLLGYSTQPRRPKHAKQYADLGQIIQEALRRYIEEVQTGQFPGPEHSFS"
     gene            complement(8050..8655)
                     /locus_tag="PLD05_08360"
     CDS             complement(8050..8655)
                     /locus_tag="PLD05_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOP77492.1"
                     /translation="MIDVRAITELHQETVQRWHHQGIDNPYTGFLALVCEQHQRNYLL
                     WHEEDKARDPQATDVQIAAVKRRIDRLNQERNDWIERLDEAIMKALAAWGVILRPRAR
                     LNTETPGSAIDRLSILALRIYHMEEQTRRSDVDEAHRAKAREKLEILRRQHADLSRAL
                     AELLADIFAGRCEMKIYRQFKMYNDPAYNPYLYQTGQRRAA"
     gene            complement(9287..9496)
                     /locus_tag="PLD05_08365"
                     /pseudo
     CDS             complement(9287..9496)
                     /locus_tag="PLD05_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913640.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10380..13538
                     /locus_tag="PLD05_08370"
     CDS             10380..13538
                     /locus_tag="PLD05_08370"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOP77493.1"
                     /translation="MPIRFLYGLKAALGTRFRKTGVRPAKRRVQSLFRLARGVEPLES
                     RHLLSASPLNSFAGDVVAGWALGGQAFDDARSIAVDSQQNVIVAGSTFSGGWTSAGYD
                     TTWGGEGDAYVAKFTRDGQHLWSTYLGGAYDDGITALAVDEEGFIYVAGWTRSSGWVS
                     GGYDTSYGGSGDGFVVKLSPAGEYLWATYLGGTNWDAISALCLTPDGKIAVAGTTSSS
                     GWIRKGFDATYNGGTFDAFAAVLTRDGEFLWSTYLGGTGWDYGYAVSTTAEAVYVAGK
                     TSSANWVSQGFDVIYAGGNFDGYLVRLSLAGQHVWSTYLGGTGEDTVASLVSDSGIVW
                     AAGSTTSADLPFASVQEGQDGWDGFVVAVQENGQPLWGTRLGGTATDQATAIARAPSS
                     GLIVVGTTTSDGWLSGGRRDVPSGESDGFIARLCATSGAVLWGSYFGGAQEDVPLAVT
                     LGTNGSVLVAGMTDLSDWLPDEPWGNLGEMNAFVAVITANRAPEITAFSADRLTIAAP
                     TFVELRVLGISDGDGPADVSGVLFYRDTNGDGLWNEPDRLLGETRDIIDGKATWILEI
                     TEGAWPLGQHTVFAVAYDHAGQTSHPAVLNLTVTKLVELGPVDYTTVTESEFSAGVVV
                     YHFLALHDALLILSLQDGVSYGLQCVLYDGNPLEDPAAIPLGAANLCPDNRTASVGHL
                     NSGEDAYLLVRTAAEQSSWIMVNLYEERASNTHIFWDSLENDAFSLLWENDPIEGVTA
                     RVTINSVDISFALQEAAETQIVFHSQWGIDTMTIEGTPADDSLQGWLDRVVFFPGGFG
                     EGETGHNVRVEVNGFDFLHAYGRGGNDRASIYDQTSTAEATAKIKLKSEPQFNHVKVI
                     GPQTFIRVKLFETVEVFADGAGDQALFYDSTGDDFFIGFLGYSQMTGPGYEVKAYGFP
                     FVTAYSKNGGNDQVRFVDSVMKDEFSLKPHKSEVFDQVTGGAVYRIVARGFDRVQVEA
                     PSATGGRDKAAIWDTIFSDNVEVFGDRLTFTRTVSRPEVIFDIRGVEFVKIRPSSGGD
                     DRVLIRIPPEIELVIGDGWQIL"
     gene            complement(13579..15915)
                     /locus_tag="PLD05_08375"
     CDS             complement(13579..15915)
                     /locus_tag="PLD05_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77494.1"
                     /translation="MRAFVHSLMERYSGRTWQLVTLIAGLVLLLAGLATARFGLSSGS
                     PVVRAAEPHPAQPPMDPERSPTPAPLPDAEKAVPLSAQAPSKGAAIRLSTSGRTQDTI
                     PPEPRPTLAGGIPSAANVAWNDVNTPMPGESATVEAHNGESIPSGSPWVVPPMPDAGA
                     VQAGQNQSPAPRTELQRPTFAGQPRPNERPAGQDVADGADTLRNPPSETPPAESNPVS
                     GGQSAPPQPLASSQGEITSLGPTQSGNAAPGAPAATPSSPFDALPPPSSGTRQAIEGT
                     TGGLSSIGAPAPIPAATAGAANPLVMADGSGVGTEGDARPGDPALEGPQSPQVSIHKL
                     MPEEVQVGKPALFRIVVRNSGAITIPKVEVHDPIPWGVRVLRTNPSATQGSQGTLVWT
                     LGPLHPGDEAVLEAEVMPLQEGELGSVTTVLVHTQASARTKCTRPQLTVKTQAQPQVM
                     IGDEVLVTMTVSNTGSGVATNVVLEEHVPPELQHPAGNELENVLGDLKPGESRQVQLK
                     LQAIRAGNAVNRLIARADGNLRVEDQTTIQVLAPQLEVTVGGPSRRFLDRETSYQIAL
                     VNRGTAPAKNVRLTASLAAGLQFVRTNNAGAYDPTTHSVQWLLAELPVGQTGMVELVV
                     NPIAAGQQELVCSVRDELGISSESRLSVLVEGISALHFQVADTKDPIAVGMETTYEIR
                     VVNQGTKEATNVQVMVTFPEGIRPLAGEGPVPNTVNGNEVLFGKLNRLAPRGEAIFRI
                     RSQAVGPGDQRIRVQILSDDIRLPITKEESTQVYSDSE"
     gene            16711..18132
                     /locus_tag="PLD05_08380"
     CDS             16711..18132
                     /locus_tag="PLD05_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77495.1"
                     /translation="MARENQALQIALITFVILTLLLGVTTYLFFRQYDEQFRKTQTAL
                     EEAAKANDAARSAQTDLNTLKTLVGFATTEPMDRISQQAKQELETYGSDLDPSQQNYR
                     AVLDHLFKTLQAKSATLVATEQKLRDAETKIAQLEASKAPLIKQQQDRADTSEATLAQ
                     ERQKFTTDLQASFKARDDLVNQVTEARQQAQDAVAQMEAAKKDFETRMRTASALLVAR
                     TRELEATKNPRLETPDGKIQWVNQALGTVWIDLGEADGLSVGTMFAVFPGDTVNVADA
                     ERKGGIEVTAIRGPHLAEARIIDDRIGNPIMPGDVISSLIWSPGDRRRFALTNFIDID
                     DDGRSDNELLISIIRSSNGIVDAWLDDDGTLHGSITADTDYIVVGDEPPENASVTAIQ
                     ARSDLLKRADEMAVLRISVRDLLRRIGYKRETHTVRFGAGANPADFRAKMPEDQVIRR
                     SGGAVSEVFQPRQPPRAKAGTAF"
     gene            complement(18209..19102)
                     /locus_tag="PLD05_08385"
     CDS             complement(18209..19102)
                     /locus_tag="PLD05_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOP77496.1"
                     /translation="MSKTTARLCVLAAVILWSSSGFFTKSHFFDDWPVEWRGAMFGFW
                     RAVAATCVLLPAVRGVRFRPMLIPMVISFAGMNITFLSAMVQTTAANAIWLESTAPFW
                     VLLASVLIFRERLRKREFVPFIAAFLGVGLILGFELTHATQTSRMGVVWGVLSGVCYG
                     GVLVTIRQLREENHAWLVALNHAAAALVLAPVVVGLGYWPTPFQLLWLTAFGVFQMAL
                     PYILLTIGLRTIPSHEAALIGLVEPILNPIWALLVWGEYPAWWTLAGATFILVGLGLR
                     YAVLERLPRDDEPQPAVCEKS"
     gene            complement(19231..>20496)
                     /locus_tag="PLD05_08390"
     CDS             complement(19231..>20496)
                     /locus_tag="PLD05_08390"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP77497.1"
                     /translation="FDLWMDPKGMTIPLTVHRRAWMAEQHVGEVANRLLQRGFAFDFI
                     SDRMIAELKASSGIIEAPGGVYRALLIPACQYMPEETLGHLADLAEAGVAVIFERQLP
                     KDVPGLSDLPARRARFAEHRGRLEQARTIAEDIEKALIAAGAIREPMADSGLRFVRRK
                     IGQDTWYFVANHTPQPVDGWMPLGISGQGVTQFDPMTGHSGRLACRQRDGMMEAYLQL
                     EPGCSTFLRVAPEASSGPTWRYLRPKGEPTVLTGTWSVEFIEGGPTLPKSYQTDKLAS
                     WTEAPDTAAQAFAGTARYTLEFTAPTETGTEWLLDLGDVRESARVRLNGRDMGTLIAL
                     PFRVCLGDALKPGHNRLEVEVTNLSANRIRDMDLRKVDWKIMKDINIVNVNYQKFDAS
                     QWPIEPSGLLGPVRLIPLEHFDPTGSGNP"
BASE COUNT         4272 a         5769 c         5924 g         4431 t
ORIGIN      
        1 tgagggcagg gagggacgcg gtcccccgcg tccgtcattt agagcctatc ccaaagttcc
       61 ctcccttcgg gagggggttg gggtgagggg aagcgaccga ggaagtcgat cgagaaaacc
      121 gacctcgatt cgcgccaccg tccccccccg gcccctctcc cgccgaacgg gcgaggggag
      181 attatgggat aggttcccat cagagatact gctaccagct tgagcctcct acgacggctc
      241 aagaacttcc ggggaattcc cgaccgactc gatagtcgcc tgcaacctat cgggagagat
      301 gatgaagatc cctcccactt ctgggtggtt ccggcagtac gattcggctt tctcgggtcc
      361 cagcagaaag aaggccgtgc ccagggcgtc ggcttcctgg gctgtcgggg caaagaccgt
      421 gacgctcaag gcgttctccg cggggtaacc agtccgcgga tcaatgatgt gtccgtatcg
      481 tttccctttg tgatagaaaa actgacggac tgttcccgac gtccccagtg cccggtcaca
      541 gatacacacc cgacccactt gagcaccttt tttcagggga tgggccaaac cgatgggcca
      601 gcccggtgag gcttgaccgg tggccgcatc aatctcccac gaacgccccc tagccaagac
      661 actgcttaag ccgccgtgga ggacaaaatc tgcaataccg ttggagagga gccgttcggc
      721 catgcgatcg agggcataac ctttaccgac gcttcccaag tttaacccca cgcccgactg
      781 aagaaatcgg attgtgtggt ctcgctcgtt gagctgaacg ttgtgccaac cacaggcctg
      841 acgggcttga gcaatttccg attcggaagg aagcctccct tcgcggcggg caaatcccca
      901 ggtctgccac aaggcggctg ccgtaacatc gaaggcacct tctgtttcct cccataacgc
      961 tcgacaccaa acgagtaaat cccacaaggc gtcggggacc ggcacttcca ccgttgccgc
     1021 caaggcgttg attcgaccta aatcgccggc cggccgaaag aaagacatca tattctcaag
     1081 cggttccaat aaatcgaggg cctccagggc ggcctcgaca gcatgctcat actgccccag
     1141 attaaggacc acttgaaact cgctacccat cgccggccga gatagcctca aatgataccc
     1201 accctgaggc cggtccttcg atacggtggg ccgatggctc tgagacgatt ccgccgaggg
     1261 cagcgacggt gaatcatcga ggatctcacc acgaacgaaa tgttccaaaa aattgcggcg
     1321 gtttcttttg gcagccatga atcaaatgca atataagaag gtcagatgtg tccactgcgc
     1381 gcaagcgaga cgacagcttt aattcgcctc agccgagttg cggctcttcc gcaggcttaa
     1441 tatcaattgt acctcgccga tgggcgttcc cgttcgctgg gcgatttcct caggggagta
     1501 accgtagtcg gccagggtat agatttcctc cacggaccgc cgtggatggc ccttgtcggg
     1561 caactcacca gtgctaggcg atgaggaaac gcttgcgggg gcctcggcgg ttattccgtc
     1621 ctgcaaattc gcctgggtaa cctgaggttg agggatgtgg gggttgacgt tacctgccgt
     1681 ttggtctttc tccgcggtgc cggccggggg gttaatttct atggcgggat gagccgaggc
     1741 cggcaacatc ccggcggcct gtgtcttgcg gatggactcg gcggtgcgga gggccgactc
     1801 cagccgggcg gccgcacgat ctgcctcttg gatgagttgt tcaaggagtc cgagcttact
     1861 gttgattcgc ccttcaagct ctctggttag ctcgtgcatc cgaacctccc actgctccaa
     1921 ggagctagat aaggcccgac ccagcgagct gctacgcagg cgttcttctg ggttgtcaaa
     1981 ttcggactcc tggtcacgag gggcaggccg tcgtcgataa gcgcgggaaa gggaaaacca
     2041 gatgagaatg atcgccgcca gtacgagcca aacagatggg ttggtccaac cattggcgag
     2101 caatacgtag gtcatggagt tttctccttg ggcggagttt cggccgtctg cccgtggttc
     2161 gcactatttt cacggaaggc cccttccgca gcttcgtaga cgacacgcag cgggaggttc
     2221 ttttccgccg caagtttccg acaggcctca aactccggcg aaaaacgggc ttcgccgtcc
     2281 ggcaaaaagc tgaccttgcc ctggaccggg ccatagggtg tttgcacgac gtgaggtcgc
     2341 cgggccaaaa tgtggcgatc gaccggccaa aagcggatgc ccaaggtcgt cgtttcccgg
     2401 aaaaggacct gttccacctg cgggatgaga tgaccagggc agaggattgt caattgcacg
     2461 gccggacggt tcttcttcat ctgaatgggc gtgacgaaaa catctagcac atcgagcttc
     2521 caaagctgtt ccacaacgta gccgatcacc tcgccagagg catcgtccca attggtctgg
     2581 accatccaca cgcggtccgt ctcaactcct ggcatcgctc cttgtggtac taaggattta
     2641 cccacaaaaa gccgcaaaac gttggcccga ccaggaatat cccgcgaacc ggctccgtaa
     2701 ccgatctgct cgatctgcat cgcgggaagg ggaccaaacc gcttggcaaa ggtcgtaagg
     2761 atcgctgccc cggtcggcgt cgtcatttcc atcatgacat cgcaaggaag aagcggcaca
     2821 ccgcggagta attcggcagt cgccggggcg ggcacggcca cctcaccatg ggcaatcttc
     2881 accgtcccag caccggtcgg caccggcgag gcgtaaacct cctggatgcc cagcaggtcg
     2941 agcccgacca cgctaccgac cacatcaaca atcgaatcgg cagcaccgac ttcgtgaaaa
     3001 tggacctttt cgatgggaat gccatgcaca cgggcctcgg cttctgccaa gcggacaaaa
     3061 accttttccg cccattcctt ttggcgaggc gtcaaatcgg cacgttccac catctccaag
     3121 atgtgatgga gatgccgatg gtggtgctcc tcgggggcct cgaccacaac ctgcgtcgcg
     3181 cgaaagcccc cttttcgcac ggtctgggca ttaacgcggc aattgggaat gcccaacgtg
     3241 gatacgacgt gctgaaggtc ctccagcttg agcccggcat ccaccaaggc ccccaacacc
     3301 atatccccac tgatgccgct aaaacaatcg aaataggcga tagtcataga aggatgcttt
     3361 ctcgtcgtgg gtcagctcag gcaacaaaaa caatcacgat ggtaatcgcg tcaaaagtcg
     3421 cggtgcagtc aagagtttct gacgtttttg gactccgatc cgtctccgct gattctaaac
     3481 atccctgccg ttgtcgcaag tgcagtgcgc cataccgccc cgatgtactg gacttcaccc
     3541 agcgttgatc ttgacatggc acggaaaata aggactccct tcccttgaag ctggggagat
     3601 ttcgggaaaa gaggtgcaac aatggcaagg aatggacatg gttaaactct ccgtcgtaca
     3661 taatgcttct ctgcattcct tctacgagct ctttagcctt cctagctgcc agaatccttg
     3721 tcgtttccta tgcgtttcga cgacctcttt gcaaaccacg atgcaagaat gggtcacggc
     3781 gtggttgtcc ctcggtttgc tgcttcttcg ttttcgaggt ctagcggcca cgggcttcgc
     3841 cgactctcag actaagacga ggattgttta tcggatcaaa cctaccccgg taatcgtcgg
     3901 caacgagttc tttctttctg cgaagttccc gaatgacctt attgagatgt gtaggcagcc
     3961 agacttgctc gcgaacctcg gcgattagct ctttccaacg tctcgggccc gctgacagcc
     4021 tacaacgcag ccattctgcc agcggcccca agtctggctc gggagtgatc aggaattgtt
     4081 gtgccggatc atccgtcttt ctcacgtaaa atccaccgtc tggacacact ttccacatgc
     4141 acgccttcat aaggtcgcgt cctgcgtcat ggtttgtaag atgcatgagc agataacgcg
     4201 tgacatcgtt atcgcccaac atgcgaatat gtgttaccca ttttgcgcca atcttttcac
     4261 gcagcagatt gatagcttga ttggcacgct catcaaagtc atctgatgac acacgatcct
     4321 tccattcgct tccatcaaaa attgaatcta gggtgtccgc catcccactc ctcttggtcc
     4381 tgagcctctc ttgccggata gccatgtcga gttcacgcca aatgatgttg acaaagagct
     4441 cgactcgcgg gaattccatc aactcgcgaa ggacacggcc cggaattttg aagccataag
     4501 gatctacgaa gacaaatgcg ggagccattt cctgtccagc gtgccgcagg gacgtcacga
     4561 cgtcatgcaa aaccttgaaa cagtccttgc aggacgtgtt gaccactatt ttggccggaa
     4621 ggttgccgag ctgtttgatt tcgctccgca gttcacagaa attcgtttcg tcacgctcga
     4681 tgaaaaagaa cacgactttg catcgttcca agaggcgagg gcgatccttg tgttgaagca
     4741 gagtctggag ggccacaagc ggcgaaccaa gctgcccttg cagatgccgc ccgcgccctg
     4801 cgtgagtgtc gacatacact acacgactat gcgagtagcc taacttcggg aaccacccat
     4861 tgagatactc ccgaattaat tggtgcttcg tgtgttgaag gtttgaatac tctaaccaat
     4921 aggaaggaca tggatccatt gtcgacatca tcttggcccc ttgcccacag acacaactgg
     4981 tacagaggac acacgcctgg gatactgacg ccagtgccgt ccgtcaagca aggtccctgt
     5041 ctcgggtcgc ctgcccgcac tctgcttaaa gaagaatgcg acttcttggg ccaacgcacg
     5101 gtctctcagc tcgcgcaccc atgcaagttt catgggacga taacgggcgc cagattcacc
     5161 tcccacgatc agccagtgaa tgccctccag gtcaagatcg gtcaaaggtc ccaggagtgg
     5221 ctccgcggat atgaagcgaa taacggcggg aatctttctg agcacgtccg cacgccaaac
     5281 gtactcgttt agttcgatac tggtacccag ccatacgttt gcataacctt cgccccagtc
     5341 gtcgggaaga tgatctgcaa ttctttctgg acgcttcgtg agtatctgga atatcaagtt
     5401 cgggcaccgt cgaattatct gccatgcttc gccacgccac tcgtcagcag cctcatgaaa
     5461 ccaatcggac caagaacacg tgaacacgaa ttcccatttt ccctgatagg ccgcctgtcg
     5521 ttgccagcgc agaggatcat tccacgttct cgtgcgaacg actgtgtgcg gctcattgcc
     5581 atagcgcaat tgtgctgcga acatgtagca atttttacag ccggggctga tcttggtgca
     5641 accccgccat ggattccacg tgcgtttagt ccacgaaatc gtcgtgttgg cacccatggt
     5701 tcaccgtccg tctaaatcga agtccttctg gtgttgatgt tcttccgcaa tacatgtgaa
     5761 aaaattttcg cctactatga ctgaattggc caaacgccaa aatctgatca ccctgacagc
     5821 taatgatagc gtacaagagt tcatcacaac ggatcaaggg cgtcaaggca atccgttggt
     5881 gcgtggaatt cactcatttc cggttttgac tgagaaagtc ggccttcctt tgtgtacagg
     5941 aagatgaaat cttagaagga gagggccatg tgtgctgcct gcgtaggtca aatgaagata
     6001 ggctatggac tgtataacaa gggccattga aggttatatg ccttaaggtc cccaagattg
     6061 gtgcctgata ggcggcacca gcgatttctc caaaagatgt tggcgattgg gcgctacgcg
     6121 aacctgaccg agatcacctg gtggtcagct tgatcgatac acatggttgt gcgggcgttt
     6181 cgccgccgtt tgagcgcaga aaatgtcact tagggtatca aatgccaccc cgacgcttgt
     6241 aaaaactggt tactgatttg ctcgagtggc cggccatcca catcagctaa tctctggact
     6301 caaaacctaa atgctgggct tgtgggggtt ttaacattag gcgaaatcgc gagaacatgt
     6361 atccttgaat tccgcgagga cgatggggaa taataacgga aaggtgtacg gtctgcctcg
     6421 attgcccgcg tcatcaccgt cgccttcaga gcggatcaaa tcgccagccg tcagaatatt
     6481 actttgcgaa aaatcacaac agaggacctg cgaatgaaaa tgacgccacg atatcttatt
     6541 gccatgttac tcttattgcc atggctcggg tgcagccgcc cggaacgagg tgcggaaccc
     6601 caaaaacaga gttcatcttc tggtgcttcc gcggaacaaa ccttgccagc cgcggaaatc
     6661 cgggtgggtg accaaacgga tttggcccgg atcatagagg aaaatcgcgg caaggtggtc
     6721 cttgtggact tttgggcaac atggtgtccg ccgtgtatgg agctnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnngaccgc caggggcgat tgtgggccaa attcggtgtt
     6901 tcggccattg cgcccgaccc gaagcggatc gaagccgaag ttgaacatct gctggccgcc
     6961 ccggaagagg cggcatctca cgagccgacg gccgggccag aattggtcat tcccgaaggc
     7021 gcttcggcgt ccacgtcctg aggtgttcgc ccttggttga ggactgtccc atgtctgccg
     7081 agtcggcccc attgacggtt cccaagttcc gtgccctgaa gcgatcgggg cgaaaaatcg
     7141 tgatggtcac ggcctatgac tatccttcgg cggccctggc ggacttggtt ggcgtggatg
     7201 ggattctcgt gggagatagc ctgggcatgg tggtccaagg aaaggccacg acgctctcgg
     7261 taacgctgcg gcagatggtg tatcacgcag agatggtggc ccgggcgact cgccacgcct
     7321 tggtggtggt ggaccttccc tttctcagtt atcaaacagg cgtccgggat gccctcaaaa
     7381 atgctggacg gattctcaaa gagacactgg cccaggccgt gaagctagaa ggtggccggg
     7441 aacgggccga actcattgca gcgttgaccg gggcagggat tccagtgttg gcccatttgg
     7501 gactgcgtcc gcaagcgatt cgggcctcgg gcggctatcg tgtggaacgc gatcgcgacc
     7561 ggctccttgc cgatgccgag gccgtgcagc aggccggggc gtttgccgtc atcttggaat
     7621 gtattccggc ctctgtggcg gaagaaatca ctggccgcgt ggacattccc acaatcggga
     7681 tcggcgcagg acctggttgc gacggccaga ttctcgtctt tcacgacctg ctgggctaca
     7741 gcacacagcc tcgtcgcccc aagcacgcca aacagtatgc cgatcttggg cagattattc
     7801 aggaagcttt gaggcgttac atcgaggaag tccaaacggg gcagttccct ggtccggaac
     7861 acagcttttc ctgatctcgg acagcgtgcc taaaagccta tcccacaacc tcccctctcc
     7921 cgttcggcgg gagaggggcc gggggggagg gccggctcga atcgctgctg gttctctcga
     7981 tcgactccga cgatggctcg ccctcaccct aaccctctcc cagagggaga gggaattttg
     8041 ggataggctt taagccgccc gtcgctgccc agtctggtaa agatagggat tgtaggcagg
     8101 atcgttgtac atcttgaact gccggtaaat cttcatttcg caccgcccgg caaagatgtc
     8161 ggccagtagt tccgctaacg cgcggctaag atcggcgtgt tgccggcgga ggatctcgag
     8221 tttctctcgg gctttagcac ggtgcgcttc gtccacatcg gaccggcgag tttgctcctc
     8281 catgtggtaa atccgcaagg ccaaaatgga aagccggtcg atcgcactac caggcgtctc
     8341 ggtgttgagt cgtgcccgtg gccgcaagat cacgccccat gcggcgaggg ccttcatgat
     8401 ggcctcatcg agccgttcga tccagtcgtt tctttcttgg ttgagtcgat caatacggcg
     8461 ctttaccgcg gcgatttgga catcggtggc ctgcgggtcg cgggctttgt cctcttcgtg
     8521 ccagagaaga tagttgcgtt gatgttgttc acaaaccaac gcaaggaacc ccgtgtaagg
     8581 attatctatg ccctggtgat gccagcgctg gacggtttcc tggtgcagtt ccgtgatggc
     8641 ccgcacgtca atcatggctt ccttccttgg acacttcaca cgtcctgggg acgactcacg
     8701 aatgatcaaa ccttgatccg tctcggcagg atggtgcatc acacaacctc catgtcaggg
     8761 atacccaagg gcaggagtta ccacacctcg ttttcccgcc ttggggcttc gagactatga
     8821 actccctgcc gcctttcatg gggcaattct cgatcacgtt taacgtacgt aaacctccca
     8881 ttttgggcaa gagcaaatcg gtggcaggtt tcccgtgcct gtggcttttc gagatcgcaa
     8941 aaggccacca ccgcacgcca ccatgaaaac aggaggtccc cgcaaagcca agttgcggag
     9001 acctcgcagg ttcgtcgtcg cggatcagac tttaagagca ccgtttaagt gaggattact
     9061 gaacgccaga tggtggcacg gtgggcgatg gagaagtgga ggtcgtggcc tctgacaccg
     9121 acgttatccc cgccgacata gcttgacacc ccggggcgtc cgcggcaggt ggggacggtt
     9181 cgcttgggct tggggcttcc gggggaggcg ttgcgtcccc gtggttctcg gagtgattct
     9241 cgggcggagt gttgtcctga ggggcatcaa ccgcgggttt ggggcgtttt tcgtagccca
     9301 acttcttgat cacttcgagg atttcgctac acgtgggaaa catccgcccg ctagagcgct
     9361 tgtactcatc cagcgcgatc atgaattcga tttcgtccgg tgtatagtct cgctcgcacg
     9421 ttgtggggtc aatctgacga cgacggggga cctttgcccg acgatcgagc tttcgccgtt
     9481 ccaccggcac gggaatgttt tgctggcggc ggtcctcacc acttcggcga tcgggcaggc
     9541 gacgacgatc aaccgtgacg acgtattccg ccgtttggct gtgcggctcg gaaggatggc
     9601 cgacttcagg tagtttctct gtgtccttgg tcgttgtgtt ggactcttga gatgcggctt
     9661 cctgaggctt tgtttgcgaa gccacagggc tttcttgatt ccgttgccgt tttgagcctt
     9721 tccgggtctc tttggcaacg gcagcgccgt ttgccggtcg gtccttgccg gacgatgtca
     9781 cacgatcctt ggtagcctca gactttgtcg ttctcttggc ggaccgtttg gtgatcatct
     9841 tggtctggga cacgatgcga tcctcctcgg ttgctagaac catctcggta ataacgtggg
     9901 ggggttccct tataacgcga aggcacgacg aaaccgtgac cgagaacccc aattgcatcc
     9961 ggccgaatac cccacgagca cggtatgctt gaacattcgg gttggcgaaa gatgttgttg
    10021 taggcaggct agataaactc ttccgtcaag agaatataac acacgcaaat cgaggatgaa
    10081 tccttggaaa agtgaaacgg ttacaaaaac cttttcgctc gtcggtttct ttggcaaatt
    10141 cgatttttcc ggcatgaacg ccgtttctgg tgcatttgat acgtcgcctc gggaacgtgg
    10201 cttgtgatgt gatctccgat cgtgtcaagg tttgcccgac cacgacagcg cgtctttgcc
    10261 caatgccggc cggttgggct gccttacttg acgccgcccc atcgagcgaa catactatgc
    10321 cgtaatttcc aagtaacgca ccgtaaaacc tgagtgattc attatgaaag gcaggtctca
    10381 tgcctattcg gttcctctat ggattgaaag cggcgttggg gacgaggttc agaaaaactg
    10441 gtgtgcgtcc tgccaagcgg cgagttcaaa gcctttttcg ccttgcccgc ggtgtggaac
    10501 ctttggaaag tcgtcacctt ttgtctgcct cgcctttgaa cagtttcgcg ggcgatgtcg
    10561 tggccggttg ggcgttgggt ggccaggcct ttgacgatgc ccgctcgatt gccgtcgatt
    10621 cacagcaaaa cgtcatcgtg gcaggcagca ccttctcggg agggtggacg tcggccggat
    10681 atgacaccac ctggggcggc gagggcgatg cctatgttgc gaagtttacc cgggatgggc
    10741 agcatctgtg gtccacttac ctgggcggag cttacgacga cggaatcacg gccctggccg
    10801 ttgacgagga aggattcatc tatgtcgcag ggtggacacg ctcatctggt tgggtttctg
    10861 gcggatacga tacctcatac ggcggttccg gggatgggtt cgttgttaag ttgagtccgg
    10921 cgggcgagta tctttgggcc acctatctcg ggggaaccaa ctgggatgca atctcggccc
    10981 tgtgcctaac gcccgacggc aagatcgccg tggcgggaac aacaagctcc tccggctgga
    11041 ttcggaaggg cttcgacgcg acgtacaacg ggggcacttt tgacgcattt gccgccgtgc
    11101 taacgagaga tggcgagttt ctttggtcaa cctacttggg cgggactggc tgggactacg
    11161 gttacgctgt gagtaccacg gcggaggccg tgtatgtggc cggcaaaacc tcctcggcca
    11221 attgggtttc ccaaggattc gatgtgatct acgccggtgg caactttgac ggctatcttg
    11281 tccgactctc gctggccggg cagcacgtgt ggtcaactta tctgggggga acaggggagg
    11341 ataccgtcgc ctcactggta tctgacagtg gcattgtgtg ggcggcgggt agtaccacct
    11401 cggcggactt gccttttgcc agtgttcaag agggacaaga cggttgggac gggtttgttg
    11461 tggctgtgca ggaaaacggc caaccgctgt ggggtacccg tctcggcgga acggcgacgg
    11521 accaggccac ggctatcgcg cgggccccat cctcgggctt gatcgtcgtg gggaccacga
    11581 catcggatgg ttggctttct ggtggccggc gagatgtccc cagcggtgaa agcgatgggt
    11641 ttatcgcccg actttgtgcg acaagcggcg ccgttctgtg ggggagctac tttggcgggg
    11701 ctcaagagga tgttccgctg gcggtgacat tgggcacaaa cggaagtgta cttgtcgccg
    11761 ggatgacgga tctatccgat tggttgcccg acgagccctg gggtaatctg ggagagatga
    11821 acgcctttgt cgcggtgatc acggccaatc gggccccgga gattactgct ttttctgccg
    11881 accgactaac gatcgcagcc cctactttcg tggaacttcg cgtcttgggg atcagcgacg
    11941 gtgatggccc ggcagacgtg agtggtgttc tcttctaccg agacacgaac ggcgacggtc
    12001 tttggaacga gccagatcgg ctcctgggag agacgcgtga catcattgac ggaaaggcca
    12061 cgtggatact tgagatcacc gagggtgcgt ggcctctcgg tcagcacacg gtgtttgccg
    12121 tggcgtacga tcacgcaggt cagacgagtc atcctgcggt gttgaatttg accgtgacaa
    12181 aactggtgga attaggcccg gttgattaca caaccgtcac agagagcgag ttttctgcag
    12241 gtgtggtggt ctatcatttc cttgcacttc acgacgcgct tctcatcctg tccctccaag
    12301 acggtgtgtc ctacgggctt cagtgcgtct tgtatgatgg caaccccttg gaagaccctg
    12361 cggcgatccc cctgggggcg gccaatcttt gtcccgacaa ccgaacggct tcagtgggtc
    12421 acttaaactc aggagaagat gcctatcttc ttgtcaggac cgccgctgaa cagtcttcgt
    12481 ggatcatggt caatctctat gaggagagag cttcaaatac ccatatcttt tgggacagtc
    12541 tagagaacga tgccttcagc ctcttgtggg aaaatgatcc tattgagggc gtcaccgcgc
    12601 gggtcacgat caacagtgtg gatatttcct ttgccttaca ggaagccgca gagacgcaga
    12661 ttgtcttcca ttcccagtgg ggtatagaca caatgaccat cgagggcacg ccagccgatg
    12721 attccttaca ggggtggctt gaccgggtgg tgtttttccc cgggggattc ggtgaaggag
    12781 agacgggcca caatgtgcgg gtggaggtga acggtttcga ctttctccac gcctatggcc
    12841 gaggcggcaa cgaccgggca agtatttatg accagacctc aacggcggaa gcgacggcca
    12901 agattaaact caaaagtgaa ccccaattca atcacgtcaa ggtgattggc ccgcagacgt
    12961 ttatccgcgt caagttgttt gagaccgttg aggtctttgc cgatggagcg ggggatcagg
    13021 ccctatttta cgatagtacc ggcgatgatt ttttcatcgg gttcttgggt tatagccaaa
    13081 tgacgggtcc cggatacgag gtaaaggcgt atggttttcc tttcgtcacg gcgtattcca
    13141 agaatggcgg caacgatcag gtaaggtttg ttgattcggt gatgaaggac gagttttccc
    13201 tgaagcctca caagtcggag gtgtttgatc aggtcacggg cggggccgtt tatcgcatcg
    13261 ttgcccgggg attcgaccgt gtgcaggtgg aggcgccctc ggctaccggt ggacgtgata
    13321 aagccgccat ttgggacacg atctttagcg acaacgttga ggttttcggc gataggttga
    13381 cgttcacccg cacggtatcc cgcccggagg tgatttttga tatccgcggt gttgaattcg
    13441 tcaagatcag gccttccagc gggggcgatg atcgcgtctt gatccgcatc ccgccggaga
    13501 ttgaactcgt cattggcgac ggctggcaaa tcttatagcc gtgcaaacgc ggcgttttgg
    13561 tcaagatgct ccgttggcct actcgctgtc ggaatagacc tgtgtgctct cttccttggt
    13621 aatgggcaag cggatatcgt cgctgaggat ttgcacacgg atgcgttgat caccggggcc
    13681 aactgcctgc gaacggatgc ggaagatggc ctcgcccctg ggggccagtc ggttgagttt
    13741 tccaaagagg acttcattac cgttcaccgt gttggggaca gggccttcgc cggcgagggg
    13801 ccggattcct tcggggaatg tgaccatgac ctgcacgttg gtggcctctt tcgtcccctg
    13861 gttgacaacc cgaatttcgt aggttgtttc catgcctacg gcgatcgggt cctttgtgtc
    13921 ggccacttgg aagtgaaggg ccgaaatacc ctccaccaga acggaaagtc gcgattcgct
    13981 gctgatgccc aattcgtccc gcaccgagca gacaagttct tgctggcctg cggcgatggg
    14041 gttcaccacc aattccacca ttcccgtttg ccctacgggc agctcggcca gtagccactg
    14101 aacgctgtgc gttgtgggat cgtaggcccc ggcattattc gtccgcacaa attgcaaacc
    14161 ggcagctagt gaagccgtca ggcggacgtt cttggccggt gccgtgcccc ggttgaccaa
    14221 cgcaatctgg tagctagttt cccgatcgag aaatctccgc gagggaccac ccacggtgac
    14281 ttccaactga ggtgccaaga cttggatcgt tgtttgatcc tcgacccgca aattaccgtc
    14341 agcccgtgcg attagccggt taacggcgtt gcctgcgcgg atggcttgga gtttgagttg
    14401 cacctgccgg gattcgccgg gtttcaaatc gccgaggaca ttttccaatt cgttccccgc
    14461 tgggtgctgc aattccggcg ggacgtgctc ttccaaaacg acattggtgg cgacgcctga
    14521 tcccgtgttg ctgaccgtca tcgtcacgag aacttcgtcg ccgatcatga cctgcggttg
    14581 ggcctgcgtt ttgaccgtca actgtggccg agtgcatttg gttcgcgccg atgcctgagt
    14641 gtggaccagc accgtggtga cgctgcccag ttccccctct tgcagaggca tgacctcggc
    14701 ttccagaaca gcctcatcgc caggatggag ggggccaagg gtccagacca acgtcccttg
    14761 ggagccttgg gtggcactgg ggttggtacg aagcacgcga actccccagg gaatcgggtc
    14821 gtggacctca actttcggta tggtgatggc cccactgttt ctcaccacaa tgcgaaacag
    14881 ggccggtttt cccacctgaa cctcttccgg catcagcttg tggatggaca cctggggtga
    14941 ttgcggccct tcgagtgcgg gatcccccgg gcgggcatca ccctcagttc ccacgcccga
    15001 accgtcggcc atcacaaggg gattcgcggc accggccgtg gctgccggga tgggagcggg
    15061 ggcaccgata cttgagaggc caccggtggt tccctcgatc gcctgccgtg tgccggagga
    15121 aggtggtgga agggcgtcaa acgggctact tggagtcgca gccggagcac caggtgccgc
    15181 gttacccgat tgcgtcgggc caagtgaagt gatctcgcct tgtgaggagg cgagcggttg
    15241 cgggggtgcc gattgaccac cggaaacagg atttgattcc gccggaggtg tttcgctagg
    15301 cgggtttctc aacgtgtccg caccgtctgc cacatcttgg ccagcgggac gttcattggg
    15361 acgcggctgt cccgcaaagg tgggccgctg gagttctgtc cttggggcgg gcgactgatt
    15421 ttgcccagct tggacagcgc cggcatctgg catgggcggt acaacccaag ggctcccgga
    15481 gggaatagat tcgccgttgt gggcctcgac ggtggccgat tcgcctggca taggagtatt
    15541 cacgtcgttc catgccacgt tcgccgcaga gggtattccg ccggccaatg tgggacgggg
    15601 ttccggagga atggtgtctt gagtgcggcc tgaagtggaa agccggatag ccgctccctt
    15661 ggacggcgcc tgtgccgaga gggggaccgc cttttccgcg tcggggagcg gtgccggcgt
    15721 tggagaacgc tccggatcca tgggcggctg cgcgggatgg ggttcggctg cccgcaccac
    15781 cgggctgcca ctcgataggc caaaccgagc cgtcgctaac cccgccaaca gaaggaccaa
    15841 cccggcaatc agggtcacaa gttgccaggt tctccctgaa tatcgctcca tcaaggaatg
    15901 aacgaacgcc cgcacggaac gaccctccat agtctcacaa gatatccgtt tacggtgtga
    15961 atcggagaac aaaaccgccc cggaccctgc caaagacaag gacctaggca tgtgcttaat
    16021 taccaaaaat gagcgcacta ggaaagacca accgacccca ctgtctgggt ggataaaacc
    16081 ctccggtatc acgatccgcc attgtgaact aacggcctgc gaccccgcca cctaacgatg
    16141 gcggcttcgg catatatgga gcctatgcca aaattccctc tccctctggt agagggttgg
    16201 ggtgagggta agccatcgtc ggagtcgatc gagaaattca gcagcgatct gtgccaccct
    16261 cacccccggc cctctctcgt ttcggcggga gaggggaggt cgtgtgatag gctcttacct
    16321 gcggccaggt tggcgcgtcg gcttccatca ctcctcccgg gccgaacgtg taaaccggtg
    16381 ggtggaaatg cgttatttca attccgcgta gaggcggcac gcctgggcac ccgttcgggc
    16441 ctggaaaacg cacactcgat ccgcaaacgc cttcatcccg caacggggtc tccgatattg
    16501 ctggcgtttg attgacattg gtggggcggc tatttagaat cgacgtagat ttatgttgcc
    16561 cagattgcca ggtttttcaa aacgtgagac ttgaaataca ttcgagttcg cgttgacggg
    16621 gcaagggttc cggaaccatc gttgttgggc gagtagcatt ggggctggag atcgactgac
    16681 ccatcgagga cgctcaggag caaacttgtc atggcacgtg agaaccaagc gctccaaatc
    16741 gctttgatca cattcgtgat tttgacactt ctattgggcg tgacgaccta tctcttcttc
    16801 cggcagtacg acgagcagtt caggaaaacg caaacggcct tggaagaggc ggccaaggct
    16861 aacgatgccg cgcgttcagc ccagaccgat cttaacacac tcaaaacttt ggttggtttt
    16921 gcaaccaccg aaccgatgga tcggatcagc cagcaggcca agcaggaatt ggagacgtac
    16981 gggagcgatc ttgacccgtc tcagcagaat taccgtgccg tccttgatca cctgttcaag
    17041 acattacagg ccaagagtgc cactttggtt gccaccgagc aaaagctccg agatgcggaa
    17101 acaaagatcg cgcaattgga agccagcaaa gccccattga tcaagcaaca acaagatcgg
    17161 gctgacacct ccgaggctac gttggcccag gaacgacaga aattcactac cgatttgcag
    17221 gcatcattca aagcccgcga tgacttggtg aaccaggtga ccgaggcccg ccaacaggcc
    17281 caggatgccg tagcccaaat ggaagccgcc aagaaagatt tcgagacccg gatgcggact
    17341 gcctctgcct tgctcgtggc gcggactcgg gaattggagg ctaccaagaa tccccgcctg
    17401 gagaccccag atggcaagat tcaatgggtc aatcaagcat tggggacggt ttggatcgac
    17461 ttgggtgagg cggatggact ctcagtcggc accatgtttg ctgtgtttcc cggggatacg
    17521 gttaacgtgg cagatgccga acgtaaagga ggcattgaag ttacggccat ccgtggtcct
    17581 caccttgcag aagcccgcat cattgatgac aggatcggca atcccattat gccgggtgac
    17641 gtgattagta gcctcatctg gtcaccgggc gatcgccgtc gttttgcttt gacgaatttc
    17701 atcgacatcg acgatgacgg ccgaagcgat aacgagctgt tgatcagtat tattcgtagc
    17761 agtaatggca tcgtggatgc ctggctggat gatgatggca cgctccacgg cagtatcacg
    17821 gctgacaccg attatatcgt agttggcgat gagccgccgg aaaatgcttc tgtaactgcc
    17881 atccaggccc gttccgattt gctcaagcgg gcggatgaaa tggcggtctt gcgaatttcc
    17941 gtccgcgacc tcttgcggcg gatcggttac aaacgtgaaa cgcacacggt ccgctttggt
    18001 gcgggggcca atcccgcaga cttccgggcg aagatgcccg aggatcaagt gatccggcgg
    18061 tccggcggtg cggtgagcga agtcttccag ccgcgtcagc ccccgcgggc caaggccgga
    18121 acggcctttt gatacccatc ccgcaaatag ctaaccgtag ggaatcttct aagcgacccc
    18181 gacgacctgt cggggttttt tgtctcttct aagatttttc gcaaactgcg ggttgaggct
    18241 cgtcgtcccg gggcagcctc tccaacaccg cataacgtag gccaagtccc accaagatga
    18301 acgtggcacc tgccaacgtc caccacgctg ggtattcccc ccagacaagg agtgcccaaa
    18361 tggggttgag aatcggttcc accagtccga ttaaggccgc ctcatggctg ggaatggtac
    18421 gcaagccgat cgtcaaaagg atatacggca aggccatttg aaaaacgccg aaggctgtca
    18481 accataggag ttgaaagggg gtgggccaat atcctaggcc cacaaccacg ggtgccagga
    18541 ctagggcggc cgccgcatga ttcaaggcca ccaaccaggc atggttttcc tctcggagtt
    18601 gccggattgt gaccaggacg cccccataac acacgccgga aagaacaccc caaacgacac
    18661 ccatccgcga ggtttgcgtg gcatgcgtga gctcaaaacc gagaatcagc cccacaccca
    18721 aaaaagccgc aatgaaaggc acgaattcgc gcttccgcag cctttctcga aagatcagaa
    18781 ccgatgccag caagacccaa aaaggggccg tgctctctaa ccaaatcgcg ttggctgccg
    18841 tcgtttgcac catcgcacta agaaaggtga tgttcattcc ggcgaaactg atgaccatcg
    18901 ggatgagcat gggacgaaag cggacaccac ggacggcggg cagcaaaaca cacgtggcag
    18961 ctaccgcccg ccaaaaccca aacatggctc cgcgccattc gacaggccaa tcatcaaaga
    19021 agtgcgattt cgtgaaaaag ccactgctac tccagaggat cactgcggcc aaaacacaga
    19081 gtcgcgccgt tgtcttgctc atcgtcgtgt gcacgatttc cccaaccagc gaaaggcaaa
    19141 ccctcgcaac ctatgaggcc cttaaatgcc gttcccataa ggcaaatgcc ccttgtcatg
    19201 cgaaccgaaa cagccagagg ctttgcctcg tcacgggttc ccggaccccg tgggatcgaa
    19261 gtgttccaag gggatcagcc gcaccggacc cagcaggccc gatggctcga tgggccattg
    19321 cgaggcatcg aatttctgat agttcacgtt cacaatgttg atatccttca tgatcttcca
    19381 atcgactttc cgcaggtcca tatctcggat ccggttcgcc gacaggttag taacctccac
    19441 ctccaggcgg ttgtgtcctg gttttagggc atctcctaag cacacgcgaa atggcagggc
    19501 aatgagcgtc cccatatccc tgccgttaag gcgaactcga gcactctcgc ggacatcccc
    19561 caaatccagg agccactccg tgccggtctc cgtcggggca gtgaactcca gcgtataacg
    19621 cgccgtcccc gcaaaggcct gggcggccgt gtccggagcc tccgtccacg aggcgagctt
    19681 atccgtctga tacgactttg gcagcgttgg cccaccttca ataaactcca ctgaccacgt
    19741 cccagtgaga acggtcggtt cgcctttggg gcggaggtaa cgccacgttg ggccggaact
    19801 ggcttcaggt gccacccgga ggaacgtcga gcatcccggc tccaactgaa gataggcctc
    19861 catcatccca tcgcgctggc ggcatgccaa gcgtcccgag tggccggtca tgggatcgaa
    19921 ttgcgtaacc ccctggccgg aaataccgag cggcatccaa ccatcgacgg gctggggcgt
    19981 gtgattggcc acaaagtacc acgtatcctg cccgatcttg cgccgcacaa atcggaggcc
    20041 ggaatcggcc attggttccc gaatagcccc cgccgcgatc agggcctttt cgatgtcctc
    20101 cgcaatggtc cgcgcttgct caagccgtcc gcggtgctct gcaaatcggg cccggcgtgc
    20161 gggaaggtcg cttagtcccg gcacatcctt gggcaattgt cgctcgaaga tgacggccac
    20221 ccccgcctcg gccaaatccg ccagatgccc caaagtttct tcgggcatat actgacaggc
    20281 ggggatgagg agcgctcgat aaaccccgcc cggggcctcg attatcccgc tgctcgcttt
    20341 cagctctgcg atcatgcggt cagaaataaa atcgaaggca aatccccgtt ggaggagccg
    20401 gttggcaacc tcgcccacgt gttgttctgc catccaggca cggcggtgaa ccgtcagcgg
    20461 aatcgtcatc cccttcgggt ccatccacaa gtcgaa
//
LOCUS       DAOSUT010000073        20353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1713_length_20353_cov_9.289634, whole genome shotgun sequence.
ACCESSION   DAOSUT010000073 DAOSUT010000000
VERSION     DAOSUT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20353
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_20353_cov_9.289634"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLD05_08395"
     CDS             <1..211
                     /locus_tag="PLD05_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOP77498.1"
                     /translation="EINDYCRCDVLDTYFIFLRTRVLVGQISLEEEHRIVAEAKQWLE
                     QRAATDRACRIYLDHWGDWPNPWVE"
     gene            complement(265..924)
                     /locus_tag="PLD05_08400"
     CDS             complement(265..924)
                     /locus_tag="PLD05_08400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP77499.1"
                     /translation="MLPRVLETEVMDTLEEALEYDSMDHSEVNQRFVDDFVRAGGHPG
                     VILDLGCGTALIPILLAEKLPSSRIIAVDYSQNMLLIGRQHLIDKNLSSAVALLRADA
                     KRLPFAEAMFSQVVSNSLIHHLADPKIAFSEAYRVTRPGGLLFFRDLARPTDRATLNR
                     LVQLYAGNANAHQQQLLADSLAAALTIEEVSELVADFALLEHSLTMTSDRHWTWIARK
                     R"
     gene            complement(1118..2401)
                     /locus_tag="PLD05_08405"
     CDS             complement(1118..2401)
                     /locus_tag="PLD05_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77500.1"
                     /translation="MNLKWSKQLRWLTFGCCLCAFLGYLPIAQGNEEGKGSAMTYGQV
                     RKYLAQHTKILELVNEEGGRVLVCPEWQGRVMTSSCDGLQGLSFGFINKEFIDAGQPN
                     PHFNNYGGEDRFWLSPEGGPFSLWFKHGVDQVLDNWFTPPALNEGAWTVISKPRDPFF
                     RMFRKMELVNTAGTQFRLEVTRDVRLLSENDLQRLFGGDVATAVRSRKVKSVGYETVN
                     RVVNAGEPFDKAKGLVSIWILGMFNAGPQTVIIVPYKQGDEATLGPVVKSDYFGPIPP
                     ERLKILPEAILFLGDGNYRSKIGTSQKRAKNVAGSMDFQNNVLTLVQFTMPEDPSQFL
                     YMNNMWGIPQAEPYVGDVANSYNDGPAGPDKPGLGPFYEIESLSPALELKTGEKLEHA
                     HRTLHIQGDYETLRSIAQKVLGVDLNVVRQTMFGQ"
     gene            3137..>4277
                     /locus_tag="PLD05_08410"
     CDS             3137..>4277
                     /locus_tag="PLD05_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77501.1"
                     /translation="MKKPKNVLILFSDDQRFDTIRALGNQEIETPNLDRLVHEGFAFT
                     HAFIMGSMQGAVCVPSRAMFLSGRTLWRVPENLSGVPTWPEVFKNHGFVTFGTGKWHN
                     GRESFARSFTQGAAIFFGGMSDHNQVPIFDYDPTGKYAQANVKTGNKFSSELFADACI
                     EFLKRFKGDRPFMAYVAFTAPHDPRTPPDEYRKKYDPADVSVPPNFLPEHPFDNGELR
                     VRDELLTSFPRHPDEIRRHIAEYYGMITHLDAQIGRILAALAEAGHQDDTLIVFAGDN
                     GLAIGQHGLMGKQNLYEHSQRVPLIIAGAGIPHGRSDALVYLIDVYPTVSELLNVPVP
                     DGVEGQSLVPIIYGKKTSVREAVFGAYREFQRSVRTDRYKYIRYRV"
     assembly_gap    4278..4377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4378..5445
                     /locus_tag="PLD05_08415"
     CDS             <4378..5445
                     /locus_tag="PLD05_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOP77502.1"
                     /translation="ISGSRTLEKAGLFDELQQLVMEGGLDWQIVARIHSEPTVTEVDH
                     VSDAVRALGVRQGDIILAVGGGSAIDLAKAVAAMVTQDARAPIRDYLENIGRNLKLIQ
                     PALPVVAVPTTAGTGAEATRNAVICSHDPPVKKSLRDDRIMPYLAVVDPELTVSNSRA
                     ITAAAGMDAITQLIESYVSVRRRPIPQILAPQALRIAFQCLPNAFTNPMDRTAREGMA
                     FAALISGICLANSGLGMAHGVAAALGALGEVPHGIACAVMLPVALQVNRSVCPSELAY
                     LARIAFDQKFANDEEAIDYLISEVRNLQAKLEIPDRLSKLGIHRELIPAIVNESRGSS
                     MSGNPKQLKDDELMEVLEQIL"
     gene            5761..7509
                     /locus_tag="PLD05_08420"
     CDS             5761..7509
                     /locus_tag="PLD05_08420"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOP77503.1"
                     /translation="MSHDHPTPPEVNRKLVIALVVVFLGYGLSLGLGWPQAATRMIVE
                     AESHASASAHHDSGADQHKAPAHSEESASETTSPAEDGHAEEERSKAVEDEQAGADPT
                     GRASSEEAHAGHSEGAEATSHSVTTHPPLWMVVPFVALLLIIAVFPLLPQTEHWWESN
                     LHRFYVAAALAAVTLLYYLVVYEHPVEGHWPAHYVSQPAAGLNWGNAWAVFANAIFSE
                     FIPFIVLLFSLYTISGGIRIEGDLRATPLVNSIIIFVGAVLASFVGTTGAAMLLIRLL
                     LDTNSERKHVAHTVVFFIFAVCNCGGCLLPTGDPPLFLGYLRGVPFLWTLQLWKEWAF
                     VNIALIVIYFLWDTFWFYPHEQKRDLRRDAAEVRRLRVRGLWPNLPLMIGVVLGVALL
                     DPSKPFPGTEIHPPLYLREIFQLGMVALSLTFGSYENRVANRFNYAAILEVAALFFGI
                     FITMQPPLQILHVEGPRLGLTQPWQFFWATGALGSFLDNAPTYVVFFETARTLGGHDL
                     VPPGTGVAHDLLVAVSLGAVFMGANTYIGNGPNFMVRAIAEKRGIKMPSFFGYMVYSG
                     CILLPLFVLVTIVFLS"
     gene            8024..8899
                     /gene="accD"
                     /locus_tag="PLD05_08425"
     CDS             8024..8899
                     /gene="accD"
                     /locus_tag="PLD05_08425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOP77504.1"
                     /translation="MADNPLASASETSRETNGWQNYVKRPKRGVPAGLWLRCPGCGAT
                     VFRKDVEKLLGVCPECDYHWYVSAHERLKQLLDEGTFEEWFANLESCDPLEFVDKISY
                     RERLKRERERTGLKDAVIAGTGMIRARRVAIGVTDSAFMMGSMGSVVGEKLTRLIERA
                     QKEQLPLIIVSGSGGGARMQEGVFSLMQMAKVSAALARFHASGGLYISVLTNPTMGGV
                     AASFASLGDVILAEPKALIGFAGPRTIMATIRIELPKGFQTSEFLLEHGFIDRIVRRP
                     ELKTEIARIIDYCGK"
     gene            9114..10010
                     /locus_tag="PLD05_08430"
     CDS             9114..10010
                     /locus_tag="PLD05_08430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP77505.1"
                     /translation="MVLRILQSLFSGSKSRIGPAKKKVDVAARFQILREGVAGTMSRF
                     YVAMDRERGEIVGLKILDPQKTAAYEARFKGLNKPCEGEIAMRFVHPYIVMTYEYGWT
                     TRDEPYLVMEYLEGGGLNGHLVAKSPFLFKKELLPYIRQVAEALAVVHEGGFIHRDVC
                     PRNLMFNRDFSILKLTDFGLSVPATPPFMQPGNRSGTPNYMAPELVRRYPTDQRLDVF
                     SFGVSMYEMLTFELPWERGTTGLAALQHEKPPADIRRFRPQIHPDLAEAIHACIEPVK
                     EKRCPSMRHFLEMIEHLQSTDL"
     gene            10035..10673
                     /gene="hisH"
                     /locus_tag="PLD05_08435"
     CDS             10035..10673
                     /gene="hisH"
                     /locus_tag="PLD05_08435"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOP77506.1"
                     /translation="MTNTPHEQSDVTIVDYGMGNLRSVQKAFERLGLRAEITDVPDRI
                     LIAERLVVPGVGAFGDAIQELERRGLVKPIMKFIESGRPFLGICLGLQLLFEKSYENG
                     VHKGLGLLRGDVVRFELPSEFSIPHMGWNQVRQECPTPLMRDIPDESYFYFVHSYYVV
                     PTDPEVVAGTTDYGVRFCSMIRQDNVFATQFHPEKSQSLGLQLLRNFASLVS"
     gene            10870..12237
                     /locus_tag="PLD05_08440"
     CDS             10870..12237
                     /locus_tag="PLD05_08440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP77507.1"
                     /translation="MSRARQTGFEHCALVSGLVTQEQLDEAWQRLRAKPSSQLEDVPI
                     EERLAQQLVELGYLNNWQAKQLLEGRTKFTLGPYKIIDSIGRGGMGQVFKGEHSLLGR
                     IVAVKVLPRHKSSPEAVENFLREIRALARLDHPNLVRAYDAGLDGNVVYLVTEYVPGS
                     DLRKLIRQRGPLHMHAAASIISQVASALEHAHSQGLIHRDVKPGNILVTNDGHAKLLD
                     LGLAGPLGQEAVNDPRYGRIVGTVDYLSPDHIREPHKPTPAWDIYALGCTLYYAVTGK
                     VPFPGGTTGDKLRAHCELRPLDPRRLNPELDAAFVDVMADMMAKDPRERIASAAEVVR
                     RLRPWAIGTYPLDETCFVRSQTHRSRRKLYVARPLPMDAMSLPETKPNFSPPLIDYGS
                     ENDPTSLSESSRRTEELSAPLAGMGQFTQEIRGHTLLGPLVLFLLIPAVLVGLVFLIW
                     RLLGG"
     gene            complement(12316..13503)
                     /locus_tag="PLD05_08445"
     CDS             complement(12316..13503)
                     /locus_tag="PLD05_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOP77508.1"
                     /translation="MATLPIYLDNHATTPCDPAVFEAMRPYFFEHFGNAASTHSYGEA
                     ASAAVEESRAIIAGLLRIDPEEIIFTSGATESNNLAILGTARRLSSDSHFITTAIEHT
                     SVLEPFRKLEKEGRHVTILPVRQRGDALAGCLDVERFRSALRPNTRFVSIMAANNEIG
                     SIQPIEEIAQICRERGIWLHVDAAQALGKIPFDLRKWDVDLVSLSAHKVYGPKGIGAL
                     VVRRRNPPIRIEPLVYGGGHERGLRSGTLNVPGIVGFAKAVQLAYERMNEENERLCYL
                     RNLMFELLQEALGPSIQLNGPALHLLEHRLAGNLNVQFTGIEAQTLLLHIPEVALSAG
                     SACDAFDPQPSHVLLALGLSEAEARCSLRVGIGRFNTEDEIRRATELICRAVRELSSF
                     YRQ"
     gene            complement(13528..14163)
                     /locus_tag="PLD05_08450"
     CDS             complement(13528..14163)
                     /locus_tag="PLD05_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOP77509.1"
                     /translation="MASLDTNEKVLVIPTSLFRKLGYFQGFSGDIANYIDDISNPANT
                     MFRPRGEVEPDPQFKQLIPYVIICHNQGQTWQVFSYTRGTGQGESRLHRKRSIGIGGH
                     ISDRDLTEGGNIVFQAIERELNEEVAVNSSYRMECVGLINDDTTDVGRVHLGIVYRCI
                     VSSPAVVPREKEIVSHGFLPVWELVKHWNEFETWSQIALKGVFGVEPPSLA"
     gene            complement(14235..15281)
                     /gene="queA"
                     /locus_tag="PLD05_08455"
     CDS             complement(14235..15281)
                     /gene="queA"
                     /locus_tag="PLD05_08455"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOP77510.1"
                     /translation="MLDTDCYDYELPKHLIAQSPLPNRADARLMVVNRATGEIEHRHV
                     RDLPELLRPGDALVLNNTKVVPARLVGYRTATGGRWEGLFVEADASGVWHVIGKTRGK
                     LQPGESITLVNLQGRDDIRLFVIGRIAEGGWLVSPDTAEDSWEVLNRVGRVPLPPYIR
                     GGQMLEEDRNRYQTVYAEVPGSVAAPTAGLHFTQGLLRKLKEAGIEICYLTLHVGLGT
                     FVPIKTSRIEEHRMHAEWGTISEEVVERLKQIRSSGGRIVAVGTTVTRLLETAALDGE
                     LKAFSGRTDLFIRPPYQFRAVDVLMTNFHLPRTTLLVLVRTFGGDNLIKRAYEEAIRE
                     SYRFYSYGDAMLIF"
     gene            15932..17008
                     /gene="prfB"
                     /locus_tag="PLD05_08460"
                     /pseudo
     CDS             15932..17008
                     /gene="prfB"
                     /locus_tag="PLD05_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            17129..17530
                     /locus_tag="PLD05_08465"
     CDS             17129..17530
                     /locus_tag="PLD05_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77511.1"
                     /translation="MDVERLRSLRFEYENQFVIVEGDRPELLRFKGKIGQVKTVNCNG
                     RALVQFDSGADRGWYDIDLDFLRVVDPPVNKGDSAESPASPDRLSQEVDYRPSALELA
                     RQAKGQSSSVEKNPLRDVRSQGTVGKKDESR"
     gene            17598..17921
                     /gene="cutA"
                     /locus_tag="PLD05_08470"
     CDS             17598..17921
                     /gene="cutA"
                     /locus_tag="PLD05_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOP77512.1"
                     /translation="MKQEALQVMVTFPSRESAESVAKGLVENRLAACVQILGPIDSIY
                     RWQGQIESAKEWLCLLKTTREAFEKLADFVRKQHPYEVPEIIGLPICQGFPPYMQWLF
                     QSIEG"
     gene            complement(17996..19426)
                     /gene="dnaB"
                     /locus_tag="PLD05_08475"
     CDS             complement(17996..19426)
                     /gene="dnaB"
                     /locus_tag="PLD05_08475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOP77513.1"
                     /translation="MAGDDSRRTQDNQLSQLSDLVGRVPPHSVEAEKAVLGSILLDPR
                     VLDDVATQLTADDFYVDAHRRIYARILEMHSENRTIDLMLLVEELRRHSEFEAVGGAA
                     YLAEIAQSVAVASHAVYYAEIVRNHALTRSLIHAGTDIVRDAFDLSLEPREIINRAEE
                     RIFAVRDRRSSGDAVPVNDVLFEAFQMIDRRMQGEATGVPTGFTDLDKLTGGLHDSEL
                     IILAARPSMGKTALATNIAEHVSVDSGLTTLFVSLEMSRLELVQRMLCSRGKINGHKF
                     RSGFLSAEDREKLVRAAGELSRGQLLIDDSPSKTVTEIAAVARRLKRRAGLRLIVIDY
                     LQLIEPDNPKDPRQEQVAKITRRLKTLARELKVPILCLAQLNRQTEVAKDNRPRLSHL
                     RESGAIEQDADVVMFVHREEYYHTKEEAQARGLCGLAEVIVAKQRNGPIGDVKLTWRE
                     EYTRFENAAAGDFAEVDYVPYEDSPF"
     gene            complement(19707..>20353)
                     /locus_tag="PLD05_08480"
     CDS             complement(19707..>20353)
                     /locus_tag="PLD05_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOP77514.1"
                     /translation="ITGYWRLFEFYGQDISQLEGEERARAEQLAKKRNEFIHLVRESV
                     VQKKTVVILDSGDPLIYGPWAWCLEEFEDLKPVVVPGVSSFNAANAALRRGVTNASKT
                     KSVILTANDWPGKTDTIEKLSSLQATMVIFTMRAEFDYFIRQLRTHYPPETPIAVVKY
                     AGYVDREEVVQSTLGRVYEQIDPAALPFEYLIYVGDFLTYRHKSSQPVTSPARE"
BASE COUNT         4461 a         5374 c         5697 g         4721 t
ORIGIN      
        1 cgagattaac gactactgcc gttgcgacgt gctcgatacc tatttcatct tcctgcgaac
       61 tcgggtgctt gtcgggcaaa tcagtctgga agaggaacac cgcattgtgg ccgaggccaa
      121 acagtggctt gaacaacgcg cggctaccga ccgcgcctgc cggatctacc tcgatcattg
      181 gggtgattgg cccaatccct gggtggagtg aaaaacggcc tcgtaaggca catgctaggg
      241 aacctctagt caccccgctt gagattatct ttttcgggca atccacgtcc agtgccgatc
      301 actggtcatg gtaaggctat gctccaagag cgcgaaatcc gcaaccaact ccgacacttc
      361 ctcaatggtc aatgccgccg cgagcgaatc ggccaatagt tgctgctgat gcgcgttcgc
      421 attgcctgca taaagttgaa ccaaacggtt cagagtggcc ctgtcggtgg gccgtgctaa
      481 gtcgcggaag aacaacaaac caccaggcct tgtgactcgg taggcttcgc tgaaagcaat
      541 tttgggatcc gcaagatggt ggatgaggct atttgacaca acttgggaaa acattgcctc
      601 ggcgaaggga aggcgtttgg catcggcacg gagtagcgcc acggcggatg aaagattctt
      661 gtcaataaga tgctgtcgtc cgataagcag catattttgg gaatagtcca cggcaataat
      721 ccgggacgag ggaagcttct cggccagcaa tatgggaatc agcgccgtac cacagcctaa
      781 gtcgaggatc acaccgggat ggccgcccgc tcggacaaaa tcatcaacaa accgctggtt
      841 gacctcgctg tgatccatgc tgtcgtactc cagcgcctct tccagcgtgt ccatcacttc
      901 cgtttctaaa acacggggca acatggcaga tgcgtggtga gttctttgga ttgctgtttc
      961 ttcgatacta cgggttctgc taaacgtggc tagttccgac gtttaataag agctttatcg
     1021 aggcaacgtc acaaacaacg ataccgccga cgcaccggcc ttaggcggca cgtcggcgga
     1081 aggacctcat cgaactgatt tatgtcttga caaagactca ctgcccaaac atcgtctggc
     1141 gaaccacgtt cagatcgacg ccgagaacct tttgggcgat cgatctcagc gtttcgtagt
     1201 caccctggat atggagcgtc cgatgtgcgt gctccaattt ttcgccggtc ttaagctcaa
     1261 gtgccggaga aagtgactcg atttcgtaga agggccctaa tcccggcttg tcgggcccgg
     1321 cgggaccatc gttatagctg ttggctacgt ctccgacgta gggttctgcc tgcgggatcc
     1381 cccacatgtt gttcatatac agaaactggg acggatcctc tggcatggtg aattgcacca
     1441 acgtgaggac gttgttctgg aaatccatcg atcctgccac attcttcgct cgtttttgcg
     1501 acgtaccaat cttggagcga taattcccgt cgcccaagaa gaggatcgct tccggcagga
     1561 ttttcaggcg ttccggcggg attgggccaa agtagtctga cttgacgacc ggtcccaagg
     1621 ttgcctcatc gccctgcttg tacgggacaa taatgaccgt ttgcggccca gcgttgaaca
     1681 ttcccaagat ccaaatcgac acaaggcctt ttgccttgtc gaacggctca ccggcgttga
     1741 ccacgcgatt gactgtctca tatccgaccg atttaacttt gcgcgatcgg acggccgttg
     1801 caacatctcc gccgaaaagc cgttggagat cgttctcact caagagacgg acatcgcggg
     1861 tcacttccag tcgaaactgg gttcccgccg tattgaccag ttccatcttg cggaacatgc
     1921 ggaagaacgg atcccgcggc ttagaaatga cggtccaagc gccttcattg agcgccggcg
     1981 gggtgaacca attatcgaga acctgatcaa cgccgtgctt gaaccagagg ctgaacggcc
     2041 ccccttcggg cgagagccag aatctgtctt cgcctccata gttgttaaag tggggattcg
     2101 gctggcctgc atcgataaac tccttgttga taaatccgaa actgagtccc tgcagtccat
     2161 cgcaggaaga cgtcatcaca cgtccttgcc attcgggaca gaccaaaacc cttccacctt
     2221 cctcgttgac aagctctaaa atcttagtat gctgtgccaa atatttgcga acttgcccgt
     2281 aagtcatcgc cgatcctttc ccttcctcgt tcccctgcgc gatgggcaag tatcccagaa
     2341 acgcgcacaa acaacaacca aaggtcaacc accggagctg ttttgaccat ttcagattca
     2401 tacatcacct catgctgcta aaactgaaga gctgccatct cgcgttgcca cccaattgcg
     2461 accgagccca aacgacttgc tattttgatc tcttttcacg gctgagtcga gataccctgg
     2521 cctagcagca tatatccctg tgccgatcaa ttcatttacg ccggcacatg tttcgctttt
     2581 ggcacgtgct tgatgggtta ctggcagcac gagtttcagt atattcgcgt tatggactca
     2641 ctttgtggct cgaaggggac gatgcgttct ctcgtccaac ttccaccggt gtagtttgag
     2701 ggccttcggg tacggctcag tcgcgctcgg cacaaaaggc ctagcacgaa caggagtctc
     2761 ttgattgccc ctcttacttt ccgcctttgg gtgggccttc aaatttgcgg agacagtagg
     2821 tatttgacat gcgctgtagt gttctcctaa actgtctaac gtttccccga gtctttcgtt
     2881 tggacaacct tcgacacatc ttcagtcgat cgcgtcctcg gcgataactc agggaacttg
     2941 tgagcagcgt cctggtttgg tttttgcggg aggaatgagg atgacagggt tttccagcag
     3001 aagagttatg ttcttgattt gttctgaaag ccgccagatt aataggttgc tcgttatttt
     3061 attgctgttg atattctcat taggcgctgt ctccggaatg cccctggcga attcgtggga
     3121 aaatagtggc gggggcatga agaagccgaa gaacgtcctg attttgtttt ccgatgatca
     3181 gcgattcgac acaattcgcg cgttgggcaa tcaagagatc gaaacgccaa atcttgatcg
     3241 gcttgtgcac gaaggctttg ccttcacgca tgctttcatc atgggaagta tgcaaggggc
     3301 cgtgtgtgtc cccagtcgag cgatgttcct cagtgggcgg acattatggc gggtaccgga
     3361 gaacctatcc ggcgttccca catggccgga ggtcttcaaa aaccatggtt ttgttacgtt
     3421 cggcacggga aaatggcaca atggtcgcga gtcgtttgcc cgcagcttta cccagggcgc
     3481 ggccatcttt ttcggcggaa tgagcgatca caatcaggtg cctatctttg actatgatcc
     3541 cacgggaaag tatgcccagg cgaacgttaa aactggcaac aagttctcca gtgagctttt
     3601 cgccgatgcg tgcatcgagt ttctgaaacg gtttaaaggg gatcggccgt tcatggccta
     3661 tgtagcgttc acagcccccc atgacccgcg gactccaccc gacgaatatc ggaaaaagta
     3721 cgacccggcc gacgtctcag tcccgcccaa ttttctccct gaacatccat ttgacaacgg
     3781 tgaattgcgt gtccgcgatg agctattgac gtctttccca cgtcatcctg atgagattcg
     3841 acgccatatt gccgaatact atgggatgat tactcatctg gatgcccaga tcggccgcat
     3901 tttggcggcc cttgctgaag ccggacacca ggacgatacc ctgatcgtgt ttgcgggtga
     3961 caatggactt gctatagggc aacatggcct gatgggcaag cagaacctct atgagcacag
     4021 ccagcgggtt ccgctgataa tagcgggggc gggcattccc cacggacgaa gcgatgccct
     4081 tgtctatttg atcgacgtgt atcctactgt gtcggagttg ctaaatgtgc cagtccctga
     4141 cggagttgaa gggcaatcgt tggtacctat tatctatggc aagaagacca gtgttcgcga
     4201 ggcggtgttt ggggcctatc gggaatttca acgatcggtc cgaacagaca gatataagta
     4261 catccgctat cgcgtcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt
     4381 tccggttcta gaacccttga aaaggccggt ctctttgatg aattacagca actggtaatg
     4441 gagggtggtc tcgattggca aatcgttgcc cgaatccatt ctgagccaac agtcacagag
     4501 gtggaccacg tgtccgatgc cgtgcgggct ttgggggtac gccaaggcga catcattctg
     4561 gccgtgggag gtggttccgc cattgatttg gccaaggccg tggcggcgat ggtcacacaa
     4621 gatgcgaggg ctcctattcg agactacctc gagaatattg gccgcaacct caagctcatc
     4681 cagccggctc taccggtcgt ggccgttccc acaacggcgg gcaccggtgc ggaagccacc
     4741 cgaaatgctg tcatttgctc gcacgatcca cccgtcaaaa aaagcttgcg cgatgatcga
     4801 atcatgccat acctggcagt ggtggacccg gaacttaccg ttagcaattc tcgggcgatc
     4861 acggcggcgg caggcatgga tgccatcacc caactaatcg aaagttacgt cagtgttcgg
     4921 cggagaccga ttccccaaat cttggcaccc caggcgctac gaattgcttt ccaatgtttg
     4981 cccaatgcct ttacaaatcc aatggacaga acggcacgcg aaggtatggc ctttgccgcc
     5041 ttgatctcgg gaatctgcct ggcaaattct ggcctaggaa tggcgcacgg tgttgcggca
     5101 gccctgggtg ccctgggcga agttccgcat ggaattgcgt gtgctgtgat gctgccagtt
     5161 gccttacagg ttaaccgaag tgtatgtccc tcagaacttg cgtatcttgc tagaattgcc
     5221 tttgatcaga agttcgccaa cgatgaggag gcgatcgatt atctgatcag tgaggtccgg
     5281 aacctccagg caaaactgga aatccctgac cggctgagca agttgggcat tcaccgcgag
     5341 ttgattccgg ccattgtcaa cgaatcccgc ggaagtagta tgagcgggaa ccccaagcag
     5401 ctcaaggatg atgaacttat ggaagttctc gaacagattc tttagttcgc ctgtgctcca
     5461 ttggtgtggg gtaaacgtcc cctcaaaagg caaacggttg ggcgggcttg tttccttaag
     5521 gccaccttgc gacggcaggt ttcaaagctt atactttcat aaccacgcaa aggcagtgtc
     5581 cgcggcagcg cgatcacccc cttgaaaaac cggtgaaaat tgtatcactt aaaagattct
     5641 agctgctaaa aggggccatc cactagtaaa ccaggcagct gagactgctc ctcagtgtga
     5701 cgttgtgcgt tagaaccacc catcgtgcaa cggattcagc aaacgtggaa ggacaagacg
     5761 atgtctcatg atcatcccac gcctccggag gtcaatcgaa aactggtcat cgctttagtc
     5821 gttgtctttc tcggctacgg cctgtcattg gggttgggct ggccgcaggc tgcgacgcga
     5881 atgattgtgg aagcggagtc ccatgcctcg gcctccgctc accatgatag cggggctgat
     5941 cagcacaaag ccccagctca ttcagaggag tcggcctcag aaacgacgag cccggcagag
     6001 gatgggcatg ctgaagagga gcggtcaaag gccgtggaag acgagcaagc tggggccgac
     6061 ccgacggggc gggcatcttc tgaggaagct cacgcgggac attcggaagg cgcagaggcg
     6121 acctcccaca gcgtgaccac tcatccgccg ctttggatgg ttgtgccctt cgtcgcactt
     6181 ttgttgatca ttgccgtttt tcctctattg ccgcaaactg agcattggtg ggaaagtaat
     6241 ctccatcgct tctacgttgc ggcggcactg gccgctgtta cgctacttta ctatctggtc
     6301 gtttatgagc accccgtgga agggcattgg cctgcccatt atgttagcca gccggctgcc
     6361 ggtcttaact ggggcaatgc ctgggctgtt tttgccaatg cgatcttcag cgagttcatc
     6421 cccttcattg tgcttctgtt tagcctctac accattagcg gtggtatccg cattgaaggt
     6481 gatctgcggg cgacgcccct tgtgaactct atcatcatct ttgtgggtgc tgtgttggcg
     6541 agcttcgtag gcacgaccgg ggcagccatg ttgctcattc ggttgctcct ggacacaaat
     6601 agcgaacgga agcacgtggc ccacacggtg gtattcttta ttttcgcggt ctgcaattgt
     6661 gggggatgtc tgctccccac tggtgaccca ccgctgtttc tgggttacct acgcggggta
     6721 ccctttcttt ggacattgca actctggaaa gaatgggcgt ttgtaaacat tgcactgatt
     6781 gtgatctact tcttgtggga cacgttctgg ttctacccgc atgagcagaa gcgagacctt
     6841 cgacgcgatg ctgcggaggt ccgacggcta agggtgcggg gcctttggcc caatctcccc
     6901 ttaatgatcg gggtggtact tggagtagcc ctgcttgatc cgagcaagcc gtttcccggc
     6961 acggaaattc atccaccttt gtacttgcgg gagatcttcc aactgggaat ggttgcgctc
     7021 tcgctgacgt ttggaagcta tgagaatcgt gtggccaatc gctttaacta tgcggctatc
     7081 ttggaggtcg ctgcgctgtt tttcggcatc tttatcacga tgcaaccgcc gcttcaaatc
     7141 ctccatgtgg aggggccccg attagggcta acccagccgt ggcaattctt ttgggccacc
     7201 ggcgcactag gtagctttct cgataacgcc cctacatacg tcgtcttctt tgaaacagcc
     7261 aggacattgg ggggacatga cttggtgccg ccggggacgg gcgtggccca tgacctgctg
     7321 gtggccgtca gcctcggagc ggttttcatg ggagctaaca catacatcgg taatggtcca
     7381 aactttatgg tgcgggccat tgcggaaaaa cgcggcatca agatgcccag tttctttggc
     7441 tacatggttt atagcggctg cattctgtta ccgctttttg tgctggtcac aatcgtcttt
     7501 ctgagttgat acagggacgt ttaagcccaa ccggtggcac tgctgtcggt gacgttgaaa
     7561 cattgcagcg cggtgttagc cgcgctgttt tgttttggtg atatggcctt cctagtcgat
     7621 gttattgcta tcattgccag agaaatatgc cgcattcagg gaaaaatcct gttgcagcac
     7681 tcgcccacat gcacaggagt ggcgagtgca aaactgggca cgagacagtt agcagttcga
     7741 gatagaagac gaaggaggct gcatgggacc cgccgagtgc cccttcttga caaagagaac
     7801 cgcttaatgg aacgataccg gcaatgcaat tgcctcaggc tgcccgatag tggggtatgc
     7861 cataccacta ggatgacggg ttgcaagacg gtggcaacct gggcatactg ccctttctag
     7921 ggctgacttg gattctttaa gaaagcccgg cgaacaaccg attgccttaa cgagataatg
     7981 acttggacgg tcattgctat cctagcataa aggcagcatc tttatggctg ataatccgct
     8041 tgcttctgca tcagagacgt cgcgagagac caacggttgg caaaattacg tcaagcgccc
     8101 aaaacggggg gtgcctgctg ggctctggtt gaggtgcccc ggctgcggcg ccaccgtctt
     8161 ccgcaaagat gtcgaaaaac tccttggggt atgtccggag tgcgactatc attggtacgt
     8221 atctgcgcat gagcggctca agcaactcct tgacgaaggc acgtttgaag aatggtttgc
     8281 caatctggag tcctgcgacc ctctggagtt tgttgataaa atctcctatc gggaacggct
     8341 gaagcgagaa cgggagcgta ctggtctgaa agatgcggtc atcgccggca cgggcatgat
     8401 tagggcccga cgggtggcta tcggcgtgac cgattcagcc ttcatgatgg ggagcatggg
     8461 ctcggtcgtt ggcgagaaac tgacccggct catcgagcgg gcccaaaaag agcagcttcc
     8521 cttgatcatt gtttccggct ctgggggcgg ggcgcggatg caggaaggcg tgttttcact
     8581 gatgcaaatg gcaaaagtct cggcggcgtt ggctcgcttt catgcctccg gagggcttta
     8641 tatctcggtc ttgacgaatc ccaccatggg aggggtcgcc gccagttttg cttcgcttgg
     8701 tgacgtgatt cttgcggagc ccaaggccct tatcggtttc gcaggtcccc ggacgatcat
     8761 ggcaacgatt cgcatcgagt tgccaaaagg atttcaaacc agcgaatttc tgttggaaca
     8821 cggttttatc gaccggattg tgcgtcggcc cgagctaaag acggagattg cccgaatcat
     8881 cgactattgc gggaaataag gccgtgaccg caagtgggca gcgcgaaccc tgggggatgt
     8941 ttcatcgggc ggggatgtgg cctgccaatg cgcgattaac tgtgagaatt tggccgggcg
     9001 tttcgctttt cttgaatggc gcccttatcg cgtctaaact tacccaaagg gcgtacttcg
     9061 acgagtgtgg ctttggctga cgtgtacgac ggctaggatt gggcactttc tccatggtgt
     9121 tgagaatact gcaaagcctg ttctccggtt cgaaatcgag gatagggccg gccaagaaaa
     9181 aggtggatgt tgcagcgcgt ttccagattc tgcgggaagg ggtagccggg acaatgtcca
     9241 ggttctatgt agccatggac cgtgaacgcg gcgaaattgt cgggctcaag atcctggacc
     9301 cccaaaaaac ggcagcgtac gaagcgcgtt tcaaggggtt aaacaaacca tgcgaaggcg
     9361 agatcgccat gcgttttgtt catccctaca ttgtgatgac ttacgagtac ggatggacaa
     9421 cgcgggatga gccgtacctc gtcatggagt acttggaagg tggcggtctt aatggccacc
     9481 ttgtggcgaa aagtcccttc ttgttcaaaa aagagcttct tccctacatt cggcaggtgg
     9541 cggaggccct agcggtagtc catgaaggcg gatttattca ccgagacgtg tgccctcgca
     9601 acctgatgtt caatcgtgat ttttcgatcc ttaaactgac cgactttggt ctgtccgttc
     9661 cggccacgcc gccattcatg cagcccggga atcgcagcgg cacacccaat tacatggcgc
     9721 ccgagctcgt gcgtcgttat cccacggatc aacgactgga tgtattctct tttggtgtca
     9781 gtatgtacga gatgttgacc tttgagctgc cttgggagcg gggaacgacg ggtttggcag
     9841 ccctccagca tgaaaagcca ccggccgaca tccggcggtt tcggccacag attcatcccg
     9901 acctggctga ggctattcac gcatgcattg aacctgtaaa agagaagcgg tgtccttcca
     9961 tgcggcactt tctcgagatg atcgagcacc tacaatcaac cgacttgtaa cgagttggaa
    10021 gcctttgaat agccatgacg aacactcctc atgaacaaag cgacgtaacg atcgttgatt
    10081 atgggatggg caatttgcgg agtgtgcaaa aggcttttga gcgattgggt ctgcgtgcgg
    10141 agatcacgga tgtgccggac cgaatcttga tcgcggagcg cttggtagtg ccgggagtgg
    10201 gggcttttgg tgacgccatc caggagcttg agcgcagggg gctagtgaag cccatcatga
    10261 agtttattga gtcgggacgt ccgtttcttg ggatttgctt agggttacaa ctcctttttg
    10321 agaaaagtta cgaaaacggc gtccacaaag ggcttggcct tttaaggggt gacgtcgtgc
    10381 gatttgagtt gccttccgag ttctctatcc cccatatggg ctggaaccag gttcgccaag
    10441 aatgccctac cccactaatg cgggatattc ccgacgaaag ctacttctat tttgtccatt
    10501 cctactacgt ggttcccacc gatcctgaag ttgtggcggg aacgaccgac tatggtgtgc
    10561 gattctgctc catgataaga caggataacg tgtttgccac gcaattccac cctgaaaaga
    10621 gtcaatcatt gggcttgcaa ctgttgcgaa acttcgcgag tctcgtgtct tgagcgtccc
    10681 aggggtagct aatattcggc gctcctcttg ctgaaggatg ggtgacattg ggtacccaat
    10741 agggcgcagc gttgagggtg gtgtgtgtac agcgccgcgt cttggcgtgt cactgttggg
    10801 gttcttctgt ggggagacgc tataatcttg aagagggcaa gtgtccgact tgggtggagc
    10861 tcctatggta tgagccgcgc caggcagact ggttttgagc actgcgcgct cgtcagtggc
    10921 ttggttacgc aagagcagct tgacgaggct tggcagcggt tgcgcgccaa gccgtcgtca
    10981 caattggaag atgtgcccat cgaggaacgt ctcgctcagc agcttgttga gttgggatat
    11041 ctcaataatt ggcaggcgaa gcagcttctc gaggggcgga caaagttcac gttaggtcca
    11101 tacaagatta ttgattccat cgggcgcggg gggatggggc aggtcttcaa aggcgagcac
    11161 tccctcctgg gacggattgt tgccgtcaag gttctacccc gccacaagtc aagcccggag
    11221 gctgtggaaa actttttgcg cgagattagg gccctagcgc gattggatca tcccaacctg
    11281 gttcgggcct atgatgcagg cctggatgga aacgtcgttt acctcgtgac ggagtacgtc
    11341 cccggatccg atctgcgaaa gctgattcgt cagcgaggtc ctttgcatat gcacgctgct
    11401 gcgtccatca tttcgcaggt ggcgtcagcg ttggagcatg ctcacagcca gggattgatc
    11461 catcgggatg tcaaaccggg aaatatcctc gtcaccaatg atggccatgc caagctgcta
    11521 gatttgggct tagcggggcc cctgggacaa gaagccgtga acgaccctcg ctatggacgg
    11581 attgtgggaa ctgtggatta tctgtcgccc gatcatattc gcgaaccaca taaaccgaca
    11641 ccggcttggg atatttatgc cctggggtgc acgctttatt atgcggtcac tgggaaggtg
    11701 ccctttccag gcggaacgac gggcgacaag ctccgcgctc attgcgaatt gcggccactt
    11761 gacccgcgtc gcctcaaccc cgaacttgat gcagcgtttg tcgatgtgat ggccgatatg
    11821 atggccaagg atccccgaga aaggattgct tcagcggccg aggttgtgcg acgcttgcgc
    11881 ccatgggcga ttggaactta tcctctggac gaaacatgct ttgtgcggtc tcagacgcat
    11941 cgaagtcgtc gcaaattgta cgtggcgcga cccctcccaa tggacgcgat gtcgctgccg
    12001 gaaacaaaac ccaatttcag ccctcccctg attgattatg gtagtgagaa cgatcccacg
    12061 tcgttaagcg agtcttctcg gcggactgag gagctatccg cacccctggc cggcatggga
    12121 cagtttactc aagagattcg cggacatacc ttgctcggtc ccttggttct gtttcttctg
    12181 atccccgccg tgcttgtggg attggtcttt ctcatttggc gtctgcttgg cggctgatag
    12241 cggttcgtag cgtttcgtga atggaatttc tcgcggttga atcactcggt ccccttgacg
    12301 tcaaatgccg aaaaatcatt gccgatagaa cgacgaaagt tccctaacgg cccggcaaat
    12361 caattccgtc gctcgtcgaa tctcgtcttc tgtattgaag cggccgatgc cgacgcggag
    12421 actacatcgg gcctcggcct cgctcaggcc gagtgccagt aggacgtgac tgggctgagg
    12481 atcgaacgca tcgcaggccg atcccgcact gagggcgacc tcggggatgt gcagcagcag
    12541 ggtttgagcc tcaattcccg taaattgcac attaagattt cccgccaagc ggtgttccaa
    12601 aagatggagc gcaggaccgt tgagctgaat gctaggcccc aaggcctctt gtagtagttc
    12661 aaacatcaaa tttcgcagat aacagaggcg ctcgttttct tcattcatgc gttcataggc
    12721 cagttgcacg gccttggcga agcccacaat gccagggaca ttcagcgtgc cactccgcaa
    12781 gcctcgctcg tgaccaccgc catagaccag gggttcgatg cgaatgggag ggttgcgacg
    12841 ccgtacaaca agcgccccaa tccccttggg cccgtaaacc ttgtgggcgg aaaggctgac
    12901 cagatcaacg tcccatttgc gaagatcaaa cgggatcttg cccagcgcct gcgccgcatc
    12961 gacgtgaagc caaatacccc gttcgcgaca aatttgagcg atttcttcga taggctgaat
    13021 tgagcctatc tcattattgg cggccattat ggagacgaat cgcgtattcg gccgcaaggc
    13081 actgcgaaat cgttccacgt cgaggcaacc tgccagcgca tcgcctcgtt gtcgtaccgg
    13141 aaggattgtg acgtggcgtc cctctttttc gagctttcga aatggctcca gcaccgaggt
    13201 gtgttcgatg gccgtcgtga tgaaatgcga atcgctggaa agtcgccgtg ccgtgcccaa
    13261 gatcgcaagg ttattacttt ccgtagcacc gctggtgaag atgatttcct cagggtcgat
    13321 acggagcagg ccggcaatga tggcccggct ttcttcgacg gcggcagagg cggcctctcc
    13381 gtaggaatgt gtcgatgcgg cattgccaaa atgctcgaaa aaatagggtc gcattgcctc
    13441 aaacaccgcg ggatcacatg gtgttgtggc atggttgtcc agatagatgg gtaaagtcgc
    13501 catgttcaac ggatcgaagc ggggaattca cgcaagcgaa ggtggttcaa ctccaaaaac
    13561 acccttgagg gcaatttggg accacgtctc gaactcattc cagtgtttga ccagctccca
    13621 cacgggcaga aaaccgtgac tgacgatctc cttttcccgc ggcaccacgg ctgggctgga
    13681 cacgatacac cgataaacga tccccaaatg gacgcggcca acatctgtcg tgtcgtcgtt
    13741 gattaatccg acacattcca tccgataact gctgttaacg gcaacctcct cgttcagttc
    13801 ccgttcgatg gcctgaaaga cgatgttccc tccctccgtc aaatctcggt cagagatatg
    13861 tccaccaatt ccaatactcc gcttgcgatg gagtcgggat tccccctggc ccgtgccccg
    13921 cgtataggag aacacctgcc aggtctgccc ctggttgtgg caaatgatga cgtaagggat
    13981 aagctgtttg aactgcggat caggttcaac ttccccccgc ggacggaaca tggtgtttgc
    14041 tggattggag atgtcgtcaa tgtagttggc gatatctccg ctaaacccct ggaagtagcc
    14101 aagtttgcgg aatagcgatg tgggtatgac gaggaccttc tcgttcgtgt ccagtgaagc
    14161 catagatatc tctttccttt ccggtatcca aatcgttgga gatccagttt cgacttccca
    14221 tgcaacaacc ctcttcaaaa gatgagcatg gcatctccgt aactgtaaaa tcggtaactt
    14281 tcgcggattg cctcctcata cgcccgcttg ataaggttat ctcccccgaa tgtcctgacc
    14341 aggacaagta gggttgtccg cggaagatgg aaattggtca ttaggacgtc aaccgctcga
    14401 aattgatacg gcggtcggat aaataggtcc gttcttcctg aaaatgcctt taattcgcca
    14461 tcgagggccg ccgtttccag aagccgcgtt accgttgtgc ctacggcaac gatccgaccc
    14521 ccagagctgc gaatttgttt tagccgttcc accacttcct ccgaaattgt cccccattca
    14581 gcatgcatcc gatgttcctc aatacgggag gtcttgatcg gtacgaaggt ccccagcccc
    14641 acgtgaaggg tgagataaca gatttcgatt cctgcctctt ttagctttcg caacaatcct
    14701 tgtgtgaaat gcaggccggc ggttggtgca gccactgagc cagggacctc ggcataaacg
    14761 gtctgataac ggttgcggtc ctcttctagc atttggccac cgcgaatata gggtggaagt
    14821 ggcacgcgcc ctactcggtt gaggacctcc catgagtcct cggcggtgtc agggctaaca
    14881 agccacccac cctcggcgat ccgtccgatc acaaaaagtc ggatgtcatc acgtccctgt
    14941 aaattgacca acgtgatgct ctcgcctggc tgaagcttgc cgcgggtctt gccgatgacg
    15001 tgccacactc cgctggcatc ggcctcgaca aaaagaccct cccaccgacc gcccgtggcc
    15061 gtccgatagc ccaccagcct cgccggaacc actttggtat tgtttaggac cagggcatcc
    15121 cccggcctaa gaagttccgg cagatcgcgc acatgccggt gctcaatttc gcctgtggcg
    15181 cggttcacca ccatcagccg ggcatcggca cggttaggta gcggactttg ggcgatcaag
    15241 tgtttaggga gctcgtaatc gtaacaatcc gtgtcaagca tttgtcaata actttaatgt
    15301 tgtatttgga gaacgaaatt taagctaacc cgcctaggtg ccctcaacaa ctttcctcca
    15361 attaccaaga tctgtccaaa ggggcgcttt aggcacgact attgccaccc aagccgccgg
    15421 cggaccgtat cgtcttatga ttctacgcca ctccctttgc cctgggtatg acatggtctg
    15481 gcggtgcgtt catcttggtg aagcgtgctt cgccgtgggc ggaaaacaat tcgggagggt
    15541 catccgaagt cttggagcag attagcctta taggcccgtg cgctgggcgg ctgaggtacc
    15601 tgatgatgga atgccgatta gtacttcggc ggacgcgacg gcatgtgtct tgcaaaggcc
    15661 cacgagattg cgtggccgaa aatccggcgt cgttccagtg atggtgctct attccccgtg
    15721 agcggctgtc acgtcagcgg cttctgggtg cgttcagata gggctgggaa acggggccga
    15781 ccattcgtta aaatgacatt aggtgagttg gaccattaac gcgaagctgc cctccagttg
    15841 gggttgatcg acagaggaga ttgtatggtc gtggaacgcg aagtcattaa tcgtgcggaa
    15901 tctattcgtg aacgcctcac gcagttaagg gactcccttt gactacgcca gcaaaaaggc
    15961 gcgggagtcc gagattgagc aaattatggc ccagcccagc ttctgggacg acattgaaaa
    16021 gtcgcaagag atggtggctg agctgaagtc gattcgcgcg attctcaagc ctcttgagga
    16081 agccatcaag cagctcgaag acatcgacgc gatggtcgaa cttgccgaag aggagccttc
    16141 actagcagac ggggttcccg acgcgctgaa caaactggaa aaacttgtcg aagatctcga
    16201 aatcaaggcg cttctcaacg ggccgcacga caactgcaac gccattgtta gtatcaatgc
    16261 ccgcgatggg gggaccgacg cccatgattg ggcggagatg ctgctccgca tgtacatcaa
    16321 ttgggcgaag gcccacgatt atgaggtcga ggttttggac cgcattgaca atgatgtggc
    16381 cggtatcaac agtgctacga ttgcaattcg cggcccgatg gcctacggct accttaaagg
    16441 cgagacgggc atccatcgtt tgatccgaat tagccccttc aatgcggagg gcaagcgaca
    16501 gaccagcttc gcggcggtcg atgtttcgcc ggagattaaa aatgaggctg agatcgaaat
    16561 ccgggaagaa gatatccgcg aggatgtctt tcgtgccagc ggccccggcg gacagcacgt
    16621 caacaagacg tcaagtgccg ttcgcctgac gcaccttccc acgggaatcg tggtggcctg
    16681 ccaaagtgaa cggagtcagc atcaaaatcg ggccatggcg atgaggatgc tccgcgcgaa
    16741 actcgctcgt cttgaggaag agaagcgaga ggccgaaatg gcggctaagt acgaagccgc
    16801 cccgaaggtg ggtttcggtt cgcagattcg cagctatttc caacatccgg agcagcggat
    16861 caaagacgcg cgaacgggat acacggagac gagcttccaa tcggtgatgg atggcgaaat
    16921 ccaaggattc ttggatgcct atcttcgttg gaaagtcgcc gaagacactg cccggatgaa
    16981 agctgctggc ggcgtcagta agaactaagg acctcggtct ctgttcgccc ttttgaccac
    17041 ccatgaaagg tgagcttctt gaatgcgatg ggacgcaata atcattctta tgcttcgatg
    17101 agataacagg ctggcggagt tgttggccat ggatgttgaa cgcttgcgga gtctccggtt
    17161 tgagtacgag aaccagtttg tcatcgtcga aggggatcga ccggaactgt tgcgattcaa
    17221 agggaaaata gggcaggtga agacagtgaa ctgtaacggc cgagcactcg tgcaattcga
    17281 ctcgggtgca gaccgcggat ggtacgacat tgacctcgac ttcctgcgag tggttgatcc
    17341 gcccgtcaat aaaggggatt ctgcagaaag ccccgcctct cccgaccgac tgtcccagga
    17401 ggtggattac cggccttcgg cgctggagtt ggcaagacag gccaaggggc aaagctcatc
    17461 ggttgaaaag aatccgctgc gtgacgtacg ctcgcagggg accgttggca aaaaggacga
    17521 atctcggtga caatttctca gggttgagag caagagttgt gccgtttcaa gcttgaccac
    17581 ggcaagggga acggaggatg aagcaagaag cccttcaagt gatggtcacg tttcccagca
    17641 gggaaagtgc cgaaagtgtg gcgaaaggtc tggtggagaa ccgcttggcc gcgtgcgtgc
    17701 aaatactcgg tcctatcgac agtatttatc gatggcaggg gcagattgag tcggccaagg
    17761 agtggctgtg tctccttaaa accactcgcg aggccttcga aaagttagca gatttcgtcc
    17821 ggaaacaaca cccgtatgag gtccctgaga taattggcct tccgatatgc caaggctttc
    17881 cgccatatat gcagtggtta ttccaatcaa ttgagggtta atgtgggcac ttggaactcg
    17941 gtccaaacaa ctgcaatacg agcaaagcga aagtaatttt gcgcggggtg ggccgctaga
    18001 acggtgagtc ctcgtacggg acgtaatcga cttcagcaaa gtccccggcc gctgcatttt
    18061 cgaaccgggt atattcttct cgccaggtca atttaacatc gccgattggc ccgttgcgct
    18121 gtttggccac gatgacctct gccaggccac acagccctcg tgcctgtgcc tcttctttcg
    18181 tgtggtagta ttcctcacgg tgaacgaaca taacaacgtc cgcatcctgc tcgatggcac
    18241 cggattctcg aaggtggctg agccgtgggc ggttgtcctt ggccacctct gtttggcgat
    18301 tcaactgggc caagcaaaga atggggactt tcaattccct ggcaagtgtc ttaagccgcc
    18361 gcgtaatctt ggcgacctgc tcttgtcgcg ggtctttggg gttgtcaggt tcgatgagtt
    18421 gcaagtagtc aatgacgatc aatcggaggc ctgcgcggcg ttttaaacgt cgtgccacgg
    18481 ccgcaatttc tgtaacggtc ttgctggggg aatcgtcaat caacagttgg ccgcggctca
    18541 gttcccctgc cgctcttacg agtttttccc gatcttccgc ggagagaaaa ccactgcgga
    18601 acttatgacc gttgatcttc ccccgcgagc aaagcatccg ttgaacaagc tcaagacgcg
    18661 acatttcaag gctgacaaac agcgttgtca agccggaatc cacggagaca tgctccgcga
    18721 tattcgtcgc cagcgccgtc tttcccatgc tcggtcgggc agctaggatg atcagttccg
    18781 aatcgtgcaa cccgcccgtc aacttgtcga gatcagtgaa ccctgttggc acccccgtcg
    18841 cttcgccctg cattctgcga tcgatcatct gaaacgcttc aaacaggacg tcgttgacgg
    18901 gaacggcatc cccggatgaa cggcgatcgc gaacggcgaa aatccgttct tctgcccgat
    18961 tgatgatctc tctcggttcg aggctgaggt caaaggcgtc acgaacgata tcggttcccg
    19021 catgaatcag cgaacgggtt aaggcgtgat tgcggacgat ctcggcataa tagacggcat
    19081 ggcttgccac ggccacggac tgggcaattt cggcaagata ggcagctcca cccaccgcct
    19141 cgaactcgct gtgtcggcga agttcttcca ccaagagcat caaatcgatc gtgcggttct
    19201 ccgagtgcat ctcgagaatc cgcgcgtaga ttcgccgatg ggcgtccacg tagaaatcgt
    19261 ccgccgttag ttgggtcgcc acgtcatcca agacccgcgg atccaggagg atacttccca
    19321 gcaccgcttt ctcagcttca acactatggg gaggtacacg accgacgaga tcggaaagct
    19381 gggaaagttg gttgtcttga gtgcgccgcg aatcgtctcc tgccaaagct aataacctcc
    19441 ttttcagcgg gtggatcaga gaattgacca gcgactgaga ccaacccagc taatcgcccg
    19501 acttcatggt tccttctaga agaccaatct agcgcgcggc gttgaaagca acaagttccc
    19561 caaattgggg acaggtgacc atcttccgaa gggcatctgt caacactacg gtcgcagatt
    19621 ggtcccacga tgcgtggaca tttggtgtca aaggatggac gctcggtact gcccgggcga
    19681 aatggcaacg agtgcggtcg aataggttac tctctggctg ggctcgtgac gggttgggac
    19741 gacttgtggc ggtacgtcag gaagtcgccc acataaatca aatactcaaa cggtagggct
    19801 gcgggatcga tctgctcata aactcgaccg agtgtcgatt ggacgacctc ttcgcggtcc
    19861 acgtagcctg cgtatttgac caccgcgata ggagtctcgg gaggatagtg cgttcgaagc
    19921 tgtcgaatga aatagtcgaa ttcagcccgc atggtaaaga tcaccatagt tgcctggagg
    19981 gacgagagct tttcgatggt atccgttttg cccggccaat cattcgcggt caggatcacc
    20041 gactttgttt tcgaggcatt ggtgacccca cgacggagtg cggcattggc agcattgaag
    20101 ctgcttacgc ccgggacgac gacgggcttt agatcctcga actcctcaag gcaccatgcc
    20161 caggggccgt agatcaaggg atccccacta tcgagaatga ccacggtttt cttctgcacg
    20221 acgctttccc gcactaaatg gatgaactca tttcgtttct tggcaagctg ctccgcgcga
    20281 gcacgttctt ccccctcaag ctgactgata tcttgcccgt agaactcaaa caaccgccag
    20341 tatcccgtga tga
//
LOCUS       DAOSUT010000074        20101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1736_length_20101_cov_9.531428, whole genome shotgun sequence.
ACCESSION   DAOSUT010000074 DAOSUT010000000
VERSION     DAOSUT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 20101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20101
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_20101_cov_9.531428"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PLD05_08485"
     CDS             complement(<1..1466)
                     /locus_tag="PLD05_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77515.1"
                     /translation="MRLGFLVPLGLAVVGFVVQAASVATSAEQGQRPNILFIMADDHA
                     YQALSCYGYGLNQTPNLDRLAKEGMRFQNCFVTNSICGPSRAVILTGKYSHLNGFARN
                     GDVFDGTQQTVSKLLQQRGYQTAVIGKWHLTSDPTGFNFWEILFGQGPYYNPPMKTPE
                     GRVQHEGYTTDVITDLVLDFLKNKRDPNRPFFVMYHHKAPHRNWCPGPEELDLFKDVT
                     IPEPPTLFDDYATRGRAAREQEMTIAKHMTPHDLKLVPQNEKFTQEQAERFYQAYAAE
                     NEAFQKANLQGEDLVRWKYQRYMKDYLRCIAGVDRNVGRVLDYLDESGLAKNTIVIYT
                     SDQGFYLGEHGWFDKRFMYEESLRTPLLVRWPGHTPPGSVCKKMVMNLDFPETFLDVA
                     GLPIPEDMQGRSLKPLLEGQDPPDWRKSIYYRYYEYPAVHMVHKHYGVRTERYKLIYF
                     HEIDEWELYDLEKDPHELRNEYNNPAYAEIVKELKAELA"
     gene            complement(1643..2902)
                     /locus_tag="PLD05_08490"
     CDS             complement(1643..2902)
                     /locus_tag="PLD05_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77516.1"
                     /translation="MNSFASRRINHCVRLRALCAVFAGVVIASFGWQNDVSGEEAGRW
                     HVGKPIVTYWAGPPLTEAVAAQLAEGGWNVVWGTEKDLETAAKHGLRLQLQDPLLSPA
                     SLDDPERRAKLDALIERVRSHPALYSYFLRDEPSAGDFPALGRLVEYLRQRDPMHLAY
                     INLFPTYATNEQLGTKGDVVTAYREHLRLYLEIVKPDLISYDHYQFRANSDGDQYFLN
                     LSLIREFALASKLPFLNIVQACSWTPSMRVPNGDELRYLVYTTLAYGAQGISYYVYCW
                     PNHVGGMANPDGTPTPLYHAAKVLNREFAAIAEELQPLTSLAVYHVGNLPPGAVALPA
                     EAPFRITSKSDAAAGFLLGYFGQADRSPTHVVVVNLNYRSPVVGELTGPAPLERFDPH
                     ERRWEPLSASTVSLDFLPGGGFLLRTR"
     gene            3604..4578
                     /locus_tag="PLD05_08495"
     CDS             3604..4578
                     /locus_tag="PLD05_08495"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP77517.1"
                     /translation="MLVRLFVVRGKANKGAVVIRLPAVIGRSRDADLTIVHPMISRRH
                     CQLFESEGLVKVRDLGSLNGTYLGGEQIQEAFLPPGSVLTIGPLVFRVEYEVSPAAIS
                     REPSVVDSHPAAPSDEMLVMEESHTVGSDSRQTGGFPTPVQAGVSGGSAEPATEPLPS
                     HFPETPVVIHESADGREPLEGGIAPPDGAMPDARAWQQVIQGDGDHSEESSEEAASGE
                     KTERNCSINTHGPYQGETRQGDQKPKGEKTPWADAPPPFTVVQKPADNASSPILLDSG
                     ADTNDDSSRRGGPAGSVFQSLGRLSSSQNVSSHTAQGARPRGWWFFGR"
     gene            complement(4610..5653)
                     /locus_tag="PLD05_08500"
     CDS             complement(4610..5653)
                     /locus_tag="PLD05_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77518.1"
                     /translation="MGTGLKRATKSIPATVGQGLPSHWRTLYICDRSRTGAWLVESFA
                     AESPAEVEVHEAPGRTAGLARLRDEIFDVIFIVHDPPELDAIDLTVAYRTGGTDEALI
                     VLGRQSEQELAVPCYEAGADGYVCVSTATSHVLKWTALRAVQRCHLTRQNRRFQQSEL
                     QRLQREREEALRVLLHQKHLISDPSSEPSTRRCEPLRTTSQRPSDDSSCDHLSQFELP
                     EPPDLPAKLIAHYRELLRAYIIMGSGHLGIELRELADVFATAGLSPKEVLHLHLYVAA
                     ELIRGLGSRSTRHVITRTNLLIMEVLLQLAEAYRSRYRDASRPPIQLFFPQFEQCMRD
                     ITASRPTIPDESC"
     gene            complement(5951..6610)
                     /locus_tag="PLD05_08505"
     CDS             complement(5951..6610)
                     /locus_tag="PLD05_08505"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOP77519.1"
                     /translation="MNRSIGCGPLGGLVFGAIFAIIGYVVAFYFGKPILDDAKASRSW
                     PSVSGVIERSEVVTSTSNGKTMYGFDVVYRYQVEGRDLTSNNVFFGGNTTSSISSFAH
                     NVVARYPKGANVKVFYDPNEPSKAVLEPGTTWQSYLVFGIGLAFLIVGVLVFISSVFY
                     VAIAGLIIGSAVAGWIGSSARRSQVADRFNLDGAPAGPQAPADRRTETTPSSDDGIDI
                     G"
     gene            complement(6618..7403)
                     /locus_tag="PLD05_08510"
     CDS             complement(6618..7403)
                     /locus_tag="PLD05_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOP77520.1"
                     /translation="MQSHEIDYQIVGDDMQFVEIELDPGETVVAEAGTMMYMEEGITF
                     EARMGDGSEPNQGVFKKLLSAAGRKLSGETIFMTHFTNTANGKRHVAFAAPYPGKIVA
                     LDLAKIGGTLICQKDAFLCAALGTKLSIEFQRRLGVGFFGGEGFILQRLTGDGKAFIH
                     ACGTIVKKKLEGETLRVDTGCLVAFTSGIDYDITLAGNLKSMFFGGEGLFLATLSGHG
                     HVLLQSLPFSRLADRILQHAPSAGGSRKGEGSVLGGLGNLFGD"
     gene            7716..8762
                     /locus_tag="PLD05_08515"
     CDS             7716..8762
                     /locus_tag="PLD05_08515"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOP77521.1"
                     /translation="MPEKVIIIGSGPAGWTAAIYAARAALNPLLFEGAISEENAKNGT
                     LPLGQLALTTEVENFPGFPPGDISRFLETALPEERRSYLPPTTRAGITGPALMELMRQ
                     QAIHFGTRVITEDIVEVDFSRRPFHLKASDGKEYQAEAVIVATGARANYLGIPSEERF
                     KNHGVSACAVCDGALPRFRNRPVAVIGGGDTAVEEATYLSHWASKVYLVHRRDQLRAS
                     KVMAERALSNPKIEVLWNRVVDEVLGDDENGVTGIRLKSTVGEPDLTIDVAGMFLAIG
                     HTPNTAFLKGQLELTEKGYIKLTRPFRTYTSVEGVFAAGDVADDYYRQAVTAAASGCM
                     AALDVERWLAGQGR"
     gene            8942..10321
                     /gene="asnS"
                     /locus_tag="PLD05_08520"
     CDS             8942..10321
                     /gene="asnS"
                     /locus_tag="PLD05_08520"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOP77522.1"
                     /translation="MEKISVAEARREDAIGREVLLQGWVRTRRDSKGGFSFLEINDGS
                     CLGNIQVVADGTLPNYESEVKRLTTGCSVAVEGVVRASPGREQPTEVYATRVTVYGWA
                     DPETYPLQKKRHSYEFLRTIAHLRPRTNSFGAVARVRNAVCFSIHRFFQEEGFLYIHT
                     PIITASDCEGAGAMFRVTTLDPATQQRLDDAADFFGRRTYLTVSGQLEAEAYACAVGK
                     VYTFGPTFRAENSNTTRHLAEFWMVEPEAAFFDLDDNMNLAERFLKRIFRDVLEQCPE
                     DMEFFNRWVDNTVIETLNKIIESEFVRLTYTEAIEILKKSGETFEFPVEWGRDLQAEH
                     ERFLTEKHFRRPVILTDYPRQIKPFYMRLNDDEKTVRAMDVLVPRVGEIIGGSQREER
                     LDVLERRMEEMGLNKQNYWWYIDLRRFGTVPHSGFGLGLERALQFITGMGNIRDVIPF
                     PRTPGSAEF"
     gene            10386..11672
                     /locus_tag="PLD05_08525"
     CDS             10386..11672
                     /locus_tag="PLD05_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOP77523.1"
                     /translation="MPDWTFPSIFRLRQKFPSHRIDDIPARIVAEFERANVRNRVRPG
                     QTVAITAGSRGIANIVLILRTVVEILHQWGARPFIVPAMGSHGGGMVEGQRGVLHTYG
                     ITEDSVGCPIRATMDTVVVGRAPEGFDVHFDRFAYEADHVVVVNRIKPHTLFVGPIES
                     GLMKMLLIGLGKHRGAEIYHRAAWDYSFDRIVRDVGRIVLSRCRILLGLAIVEDAYDA
                     TAHLEAVLPEAFETREPELLRLAKEWMPRLPFDHAHVLLIDQLGKDLSGSGIDTNIIG
                     RKFNDHRAAPGEKPDIRFICVRGLSPGTHGNAIGLGLAEFCRSDILPQIDVHATRINA
                     ITSGHVSAAMVPLDYPTDREMLGQALANIGMTPPEHARLMWIADTLHLTEVECSAAYL
                     AEAKARDDLEILTGVRPMPFGDDGNLPDMMTLRASS"
     gene            12100..12768
                     /locus_tag="PLD05_08530"
     CDS             12100..12768
                     /locus_tag="PLD05_08530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP77524.1"
                     /translation="MNRRLSAILPFRNAEHVVLNLIPSYVDVFSELSSDWEILAVDDG
                     SEDSTPEILCEFAQMYPQVRVAIHGQARGFYASLCSGLSLSVGNILFLPLRYQPMTLD
                     SLHRLWSAVQNHSLVIGLAANEFGQDEIECLMLQRRVASPILPALSSTERLAAVVNRF
                     ESSAVIVPLRRVAFSQEKPYASYGRTFTTTNGREAMPRAPKSLTLQAAKAVFSRIKQF
                     ALDE"
     gene            complement(12786..13349)
                     /locus_tag="PLD05_08535"
     CDS             complement(12786..13349)
                     /locus_tag="PLD05_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOP77525.1"
                     /translation="MFEVFEHTADLGIRVRAPSLEGLFKEAAEAMIQLMCRGPQLEDT
                     TSAPTGATPSAQAASPPTEALRATPTELREVTIPAEDLPIPEPQPSPCETSSDNGSRI
                     VLSPTWEDLLHDWLSTILYLFSVERLIPVDYQIQFDSMGLKARLKVRAYDPVRDGGEV
                     EIKAVTYHGLRIEKEANEFTAQVIFDV"
     gene            13702..15168
                     /locus_tag="PLD05_08540"
     CDS             13702..15168
                     /locus_tag="PLD05_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOP77526.1"
                     /translation="MKLCSRAGTTITLQQLLPDLQVFGDGELQVGVCHSQPSRIKPGD
                     LFVLLHPPGPRRDAAIEQAVGRGCAAILSEDVLPGLSVPVICVSDVRTAYGRICHALH
                     GFPTQKLGVIGVTGMYGKTTTSCLVARILVQAGYSVGMLTSLGYFEGEESTFPVETTP
                     PPDELAARLARMADNECTHAVVEVSAKAIKQRYTAGLEFDTVVFLNDEAEGASSHDAR
                     CRWFSLVDNLRPEGLAIFNADDSRCRAALAETDHPALTVGIHAAAEIKAREIHRHLGE
                     ETFYICVGDDTFPVRTKMFGRHHVRNCLAAVAVGLTYHVPMEVIVQAIESVDQIPGRL
                     QPVLGGQPFSVFVDAAETSTSLAAVLEAVVPQVQGRVICVASLPRNPRQELGKWLELI
                     DAHCGLGVITLANPGREDPAILSPLAYESMKSPRRIVIPDRTEAIGWALTEAREGDCV
                     LVIGNGHRRWRPLKENDLEVDDFQFIRQWLEQEFPHVT"
     gene            complement(15553..16449)
                     /locus_tag="PLD05_08545"
     CDS             complement(15553..16449)
                     /locus_tag="PLD05_08545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP77527.1"
                     /translation="MSDYQEDSDIQPSAPGAPTSATLEEALAKCQIALLPEQVRLLDQ
                     YFQLRREWNQKMNLTRHDTYDQFVARDVVDSLSFMRCLQPDEVILDVGTGSGVPGVVL
                     SILRPDLCVVLSETVGKRARAVADIVSRLGLKIPMLIGRAEKYLEAGGWEFHSLVIRA
                     VAPLLNLLRWFRPYWDCFDRMLILKGPRWVTERGQARHYGLMHGLALRVVDRYTIPGT
                     GAESVLLQICPEERLIPPRECRFRKLSCEEMITGVVESPQPGPFAKKGTVPPAAKHQK
                     KPGPKKPSVSKGPGKNPGRRPK"
     gene            complement(16525..17103)
                     /locus_tag="PLD05_08550"
     CDS             complement(16525..17103)
                     /locus_tag="PLD05_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOP77528.1"
                     /translation="MKVVAFNGSPRKDGNTAILVETTLQVLRREGIETEHISLAGQHL
                     HGCVACYRCFKEKGQCHQKDDAVNDYIKKITEAQGVILASPVYFADVTTNMKALMERS
                     GMVARAAGDLFRRKVGAAIVAVRRAGAIHTFDSLNHYFSIAQMITVGSSYWNVGRGLQ
                     PGEVSSDEEGIRTMQTLGENMAWVIKKLYGTT"
     gene            17819..>20101
                     /locus_tag="PLD05_08555"
     CDS             17819..>20101
                     /locus_tag="PLD05_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOP77529.1"
                     /translation="MPDEPTIPQGGAERRLSQAMSLQGRRPPADVPGYDPERLLGAGA
                     YGEVWVALERNTGRRVAIKFYSHRGGLDWSLLSREVEKLAFLCADRYVVQLIAVGWDS
                     EPPYYVMEFMEQGSLAERLAKGPLPATEAVRLFREVAIGLLHAHNKGVLHCDLKPGNV
                     LLDQDGKPRLADFGQSRLSHEQLPALGTLFYMAPEQADLNAVPDARWDVYALGALFYC
                     MLTGHPPHRDQETAKKIEEITDLEERLAAYRRLIEESGIPTDHRSIPGVDRGLAAILE
                     RCLAPDPNQRFPNIQSVLVALETWAAERARRPLVVLGFVGPALLLLVFALFAWRGFAT
                     TVNRSRDMLIHRALENNRLMAQYVADLAGKELRRRFEAVEQLGGNARFQDLIRQVIRD
                     PEYEELSRELSRADLPEEEAEVLRRRFRSHPLRAALQREFSALIPPWMRPEESGESSV
                     DDVASWFFCDPNGISTARVPESVTIGRNYAWRSFFTGLDRDMPREWRPAQGEHLRSPK
                     LSAVFRSEATGLWIVAVAAPVIDLRTNEFLGVVALTVRVTRFVELEGTPAQFPVLVEL
                     RPGDHTGMILQHPLFDQVLLREQRLPERFQNYRLSKDALPPDLDKGAIHDPRAIGYRD
                     PLAADPEGSLFARDYLAQAAPVRLSSGKDSQDTGWMVIVQEDYDVAVRGPLEGFQKAL
                     VRYGLISASLVAAILLGLWTLTVRLLNEIHENHLLSRSEYLLRETSRGSHRVVPRVIT
                     DHPTSTAPWVSDSIPSGPSIE"
BASE COUNT         4260 a         5437 c         5901 g         4503 t
ORIGIN      
        1 gcaagttcgg ccttcaactc cttcacgatc tcggcataag cgggattgtt gtattcgttc
       61 ctaagttcgt gaggatcctt ttccaggtcg tacaattccc actcgtcaat ctcatggaag
      121 taaatgagct tgtagcgctc tgttcgtacg ccatagtgtt tatggaccat gtgaacagcc
      181 gggtattcat agtaccgata atagattgat ttgcgccagt caggtgggtc ctgtccctcc
      241 aagagcggct tcaggctccg cccctgcatg tcttcgggaa tggggagccc ggccacgtcg
      301 aggaacgttt ccgggaagtc tagattcatg accatcttct tgcagacact tcccggcggc
      361 gtgtgccctg gccagcggac caagagcggc gttcgcagtg attcctcgta catgaatcgc
      421 ttgtcaaacc agccgtgttc gccgaggtag aagccttggt cagaggtgta aatgacaatt
      481 gtgttcttgg ccagacccga ttcatcgagg tagtcgagga cacgccccac gttgcgatca
      541 acaccggcaa tacaacggag atagtccttc atatagcgct gatacttcca gcggacaagg
      601 tcctcgcctt gaagattggc tttttgaaag gcctcgttct ccgccgcgta ggcctgataa
      661 aaacgctccg cctgttcctg ggtgaatttc tcattctggg gcacgagctt caagtcgtga
      721 ggcgtcatgt gcttggcaat agtcatttct tgttcccgcg cggcacgacc ccgtgtggcg
      781 taatcatcaa agagggtcgg gggctccgga atcgtcacgt ccttgaaaag atcaagctcc
      841 tctgggccgg gacaccaatt ccgatgcggg gccttgtgat gatacatgac aaaaaacggg
      901 cgatttgggt cccgcttgtt cttgaggaag tccagaacca aatcggtgat cacgtccgtc
      961 gtgtagcctt catgttggac gcggccttcc ggcgtcttca tcggtgggtt gtaatacggc
     1021 ccctgaccaa agaggatttc ccaaaaatta aagcccgtag gatcactggt caaatgccat
     1081 ttcccaatga ccgccgtctg atagccgcgc tgttgcagca gcttggatac cgtctgctgg
     1141 gtgccatcga agacatcgcc gtttcgtgca aacccattga ggtgagaata ctttcctgtc
     1201 agaatgacgg cccgacttgg accacaaatc gaattggtca caaaacaatt ctggaagcgc
     1261 atcccttctt ttgccaagcg atcgaggttg ggcgtttgat taagtccata gccataacag
     1321 ctcaacgcct gataggcatg atcgtccgcc atgatgaaaa ggatgttagg acgctgccct
     1381 tgttcggctg acgtcgctac cgaggccgct tgcacaacga agccaacaac ggccaaccca
     1441 agaggtacaa gaaaaccaag gcgcataatt cacctcccca aaagcgatca aagtcttgtc
     1501 gatgactcac tcaatccgtt ccgaaatcac cctaacgttg ttaccggata gtgtggcttg
     1561 agcaccggtc attgtcaacc gacgaaggga taactttacc agcggttcat aacgctatta
     1621 gataggaaac ccaacaaaac gactatcggg ttcgaaggag aaaacctccg cccggcagaa
     1681 aatccagcga taccgtggag gccgacaaag gctcccaccg tctctcgtgg ggatcgaatc
     1741 gctcaagggg ggctggtcct gtgagttccc ccacgaccgg cgagcgatag ttcagattga
     1801 cgaccacaac gtgcgtcggg ctccggtctg cctggccaaa ataccccaag aggaaaccgg
     1861 ccgcggcatc gctcttagag gtaatccgga acggcgcttc cgctgggaga gcaactgccc
     1921 ctggcgggag attcccaacg tgatagacgg ccagcgatgt caacggttgc aattcttccg
     1981 caatggcggc aaattcgcga ttgagaacct tggccgcgtg gtacagtggg gtcggcgtgc
     2041 cgtcgggatt ggccatcccg cccacgtggt tgggccagca gtacacatag taagaaatcc
     2101 cttgcgcacc atacgccagg gttgtgtaaa caaggtatcg cagttcatca ccgttaggta
     2161 cccgcatgga gggtgtccaa ctgcacgcct gaacaatgtt aaggaagggg agtttcgagg
     2221 ccaacgcaaa ttcgcggatt agcgaaagat taagaaagta ctgatcgccg tcactgttcg
     2281 cgcgaaattg gtaatgatcg tagctgatga ggtccggttt gacgatctca aggtaaagac
     2341 ggagatgctc gcggtaggcg gtgacgacgt ctccctttgt gccaagttgt tcgtttgtgg
     2401 catacgtcgg gaaaaggtta atgtaggcga gatgcattgg atcgcgttgg cgaaggtatt
     2461 ccaccaggcg ccctaaggcg ggaaaatcgc ctgcgcttgg ttcgtcccgc aagaaatagc
     2521 tgtaaagggc gggatgactg cgaacgcgct cgatcaacgc gtcgagtttc gcccgccgtt
     2581 ccggatcgtc gagtgacgcc ggcgagagaa gcgggtcctg aagttgcaag cgtagtccgt
     2641 gcttggccgc cgtctccaag tccttttccg tgccccagac aacattccaa ccaccttcgg
     2701 caagctgtgc cgccacggcc tcggtcaacg ggggacctgc ccagtaggtg acgatcggtt
     2761 tgccgacgtg ccaccggcct gcctcttccc cggaaacgtc gttttgccat ccaaaagagg
     2821 cgataaccac accggcgaat actgcacata gcgccctaag gcggacgcaa tgattgattc
     2881 tgcggcttgc aaaactgttc atagtagtgc ctcacataga aaacgggcat gtttttggaa
     2941 aagagttacg tccggttggc ctatgtgtaa cgctccccct tgctcccgtc aagcatcgca
     3001 cagtcataaa ccaaatccgt ccccactcaa aggtgcgtca agatgcactg attcgtaaga
     3061 acttagttat gtgcttgtca cttccttgtg agacttgatt cagacgctag ggcctaacgg
     3121 caagacagtc agcggcatgg tcttccagcc catgctatag gaccaaggac tccgccggcg
     3181 gcattttctt gccaggtaaa atttcctggg atgtgggcct tggcctgagg tgcgcacctc
     3241 tccacgccgc cttgtcaacc caattgccta tcccataatc tcccctcgtg ggttcagccg
     3301 gagaggggcc tggggtgagg gtggctcgaa tcgctgccgg tgttctcgat cgactccgac
     3361 gttcgcttgc cctcacccca accctctccc ggagggagag gcagtctttg ggataggctc
     3421 taagtgccta ctcccagcaa gttacttgct tctctggctt ttgccgtagg gattgtgtcc
     3481 tgagaaacgg gatggcggcg gaaaaatccc attgagggat ttgacggcgg tgggtagaat
     3541 aaattcggtt gcctttaatt acctgcccag cgacgtcccc aataaaaaag agaagcctcc
     3601 ccaatgcttg ttcggctttt cgttgtccgc ggcaaagcca ataagggtgc ggtggtcata
     3661 aggttaccag cggtgatcgg tcgcagccgc gatgccgatt tgacgatcgt tcaccccatg
     3721 atcagccgcc gccattgcca actctttgaa tccgaaggcc tggtgaaagt tcgggatttg
     3781 ggctctttga atggtactta tttgggtggt gagcagattc aggaggcgtt tctccccccg
     3841 gggagtgtcc tgaccattgg accactcgtc tttcgcgtgg agtacgaagt ttccccggca
     3901 gcgatcagcc gagaaccgtc cgtggttgat agccatccgg ctgcgccctc cgacgagatg
     3961 cttgtcatgg aggaatccca tacggtggga agtgattccc gacaaacggg tgggttcccc
     4021 acgccggtcc aagcgggggt ctccggcggg agtgccgaac cggccacgga gcctcttccc
     4081 agccattttc cagagacgcc ggtcgttatc cacgagtcag ccgatgggcg tgagccactc
     4141 gaagggggaa tcgctccacc cgatggtgcc atgccagatg cgagggcgtg gcaacaagtg
     4201 atccagggtg atggcgatca cagtgaggaa tcatcggagg aagcggcctc aggcgagaaa
     4261 accgagcgga attgctcgat caacacgcat ggcccgtatc agggggagac gaggcaaggg
     4321 gaccagaagc cgaagggtga gaagacaccc tgggcggatg cgccaccccc gttcacggtt
     4381 gtccaaaagc cggcagacaa cgcttcctcg ccgatcttgt tagattcagg agcagacaca
     4441 aacgatgact cgtcccgacg cggtggtccg gcgggaagtg tctttcagtc cctcggcagg
     4501 ttgtcatctt ctcaaaacgt ttccagccac acggcgcaag gcgctaggcc gcggggatgg
     4561 tggttctttg gacgttagtt tggacgttgg tggcgcgtgc gacctggcgc tagcaggact
     4621 catcggggat ggttggccgt gatgccgtaa tgtctcgcat gcactgctcg aattgcggaa
     4681 agaagagttg tatgggcggt cgcgaggcgt cccgataacg tgaccggtag gcctctgcca
     4741 actggaggag gacttccata atcaggaggt tcgtccgggt gatcacatgg cgggtgctcc
     4801 gcgacccaag ccctcggatc aattcggcag ccacatagag atgaaggtgg agcacttcct
     4861 tgggggacag ccccgctgtt gcaaagacat cggccagttc ccgcagttcg atgccgagat
     4921 gtcctgaacc catgatgatg tatgcccgca aaagttccct atagtgggcg atcaatttgg
     4981 cgggcaagtc gggaggctct ggcaactcaa attgggagag atgatcgcaa gacgaatcat
     5041 ctgacggccg ctgggacgtg gttcgaagtg gctcgcaacg ccgcgtcgat ggctcggagg
     5101 atgggtcaga gattaaatgt ttttgatgaa ggaggacccg tagggcctct tcacgctcgc
     5161 gctgcagacg ttgcaattca ctttgctgga atcgacgatt ttggcgagtg agatgacaac
     5221 gttggactgc ccgcagtgcc gtccacttaa ggacgtggct ggttgctgta ctgacgcaga
     5281 catagccgtc agccccggct tcgtaacagg ggacggccaa ttcttgctca ctttgccgcc
     5341 caaggacgat cagggcctcg tcggttccgc ctgtccgata agcgacggtc aggtcgatcg
     5401 cgtccagctc agggggatca tggacgatga aaattacgtc gaagatctca tcgcgaagac
     5461 gggccaggcc tgccgttcgc cctggcgctt cgtggacctc aacttctgcg ggggactccg
     5521 cagcgaagct ttccaccagc caggcgcccg tgcgggagcg atcgcaaatg tagagcgtcc
     5581 gccagtggga aggcagcccc tgtcccacgg ttgcgggtat tgatttcgtc gctcgtttga
     5641 gtccggtgcc catcggttgg cgtttcactg gtgggttgca aaatacgata cccaataccc
     5701 cattcctaca aggcgtcctg agagtagccg aattgtttcc ttcacgtcaa gaccggtttg
     5761 ctacgtcacg aatgtgaagc ttggacatag gctgtttggg ggggtgacga gcttttcaac
     5821 tcttcacgct acgccaagcc agttatcgcc gtgacgcaaa acgctaaggt cgttttaggg
     5881 cgcaaacgga ttcccatttt gggcggcttc catcgagagg cggaccgagg ccgcaccttc
     5941 tcgcggctat ctagccaata tcaataccgt cgtcgctcga tggcgtggtc tcggtacgac
     6001 ggtccgctgg agcttgcggg ccggcggggg caccatcaag gttgaagcgg tctgccactt
     6061 gtgaacggcg agcggagcta ccaatccaac ccgccaccgc gctgccaata atcaatcccg
     6121 ctatggcgac gtagaaaact gagcttatga agaccaggac gccgacgatc aaaaaggcca
     6181 acccgatccc aaaaaccagg tagctttgcc acgtggttcc gggttcaagc actgctttgg
     6241 acggctcgtt aggatcgtaa aagaccttga cattggcgcc cttcggatag cgggcgacca
     6301 cattgtgcgc aaagctggag atcgagcttg ttgtgtttcc accgaaaaac acattgttag
     6361 aggtcaagtc ccggccttcg acctggtagc gataaaccac gtcgaagccg tacatcgttt
     6421 tgccattcga tgtactggta acgacctcag agcgttcgat gacgccggaa acacttggcc
     6481 atgaccggct cgccttggcg tcgtcgagaa tcggcttgcc gaaataaaac gccacgacat
     6541 agccgatgat cgcgaagatc gcaccgaaaa caagaccacc gagcggccca caacctatgg
     6601 aacgattcat agatacctca gtcgccaaaa aggttgccca aaccgccgag aaccgagcct
     6661 tcgccctttc tactgccgcc ggccgaagga gcgtgctgga ggatcctgtc ggctaagcgt
     6721 gagaagggta aactctgcag cagcacgtgg ccatgtccgc tcagggtggc taggaacagg
     6781 ccctcgccac cgaagaacat cgacttgaga ttgcccgcga gggtaatgtc gtaatcaata
     6841 ccagaggtga atgcgacgag gcagcccgta tcaactcgca gtgtttctcc ctcgagcttt
     6901 ttcttgacga tggtgccaca ggcgtggatg aaggccttgc cgtcgcctgt cagtcgctga
     6961 aggatgaaac cctcgccgcc gaagaatccg acgccgagac gccgttggaa ctcgatcgaa
     7021 agctttgttc ccagcgctgc gcagagaaaa gcgtcttttt ggcaaatcag cgttccgccg
     7081 atctttgcca ggtccagggc gacgatcttg cccggatagg gggcggcaaa ggccacgtgg
     7141 cgtttcccgt tggcggtgtt agtgaaatgg gtcatgaaga ttgtctcacc ggaaagcttt
     7201 cgtccggcgg cgctgagaag tttcttgaag acgccctgat tcggctcgga cccgtcgccc
     7261 atgcgggcct cgaacgtgat gccctcctcc atgtacatca tggtcccggc ttcggcaacc
     7321 acggtttccc cgggatcgag ttcgatctca acgaactgca tgtcatcccc gacaatctgg
     7381 tagtcaatct catggctttg cacggcaagg ctcctttgat ctcgcggttg cacgcctaca
     7441 atcggaaatc cagcacgagg cgaacggtcg gctttgggct gtccagtcag gcgccgtagg
     7501 ctctgagatt attcgtggaa agcctatcat ccttgttcgt cgaagtctat ccagtattag
     7561 gatccgtttt ccaaaatttc gctaccaacg tcggagatga gcgaaaaagc cccgccacgg
     7621 ggtctccaac tcccgcagtg ttagccggag gacatataat gttaaattcg ttttgcacgt
     7681 tacgcttaaa ggacctgatt tgaagcgagg taaaaatgcc agaaaaggta atcattatcg
     7741 gcagtggccc ggcgggctgg acagccgcga tttatgcagc acgagcggct cttaaccccc
     7801 tcctttttga gggtgcaatc agcgaagaaa atgcaaagaa tgggactctt ccccttgggc
     7861 aactggcgct gaccacagag gtggagaact ttccgggctt tcctccaggg gatatttcgc
     7921 gttttcttga aacggcactg ccggaggagc gacgctctta tctgcccccg accaccaggg
     7981 cgggcatcac ggggccggcc cttatggaac tgatgcggca acaagcgatc cattttggga
     8041 cgagggtgat taccgaggat attgttgagg tggatttctc ccgtcggcct tttcacctca
     8101 aggcgtccga cgggaaggaa tatcaggccg aggccgtgat agtggcgacc ggtgcgcggg
     8161 cgaattattt gggaattccc tcggaggaac gtttcaagaa tcatggtgtg agtgcgtgtg
     8221 ccgtctgtga cggggcgcta ccgcgttttc ggaatcgtcc cgtggccgta atcggcggcg
     8281 gtgataccgc ggtggaagag gctacctatt tatcgcactg ggcatcgaaa gtttatctcg
     8341 tccatcgccg cgatcaactg cgggcgtcga aggtgatggc cgagcgggcc ttaagcaatc
     8401 ccaagatcga agtcctctgg aaccgcgtcg tcgatgaagt gctaggagac gacgagaacg
     8461 gcgttacagg aattcgcctc aagagtactg ttggggagcc cgatttgacg atcgacgtgg
     8521 cgggcatgtt tctggccatt ggacacacgc caaacacggc tttcttgaaa ggacagttgg
     8581 aactgacaga aaagggttat atcaagctga cccggccttt ccggacgtac actagtgttg
     8641 aaggagtctt tgcggcgggc gacgtggccg atgattacta tcgtcaagcg gtcacggcgg
     8701 cagccagtgg ttgtatggcg gctctggatg tggaacgttg gctggcgggg caggggaggt
     8761 aggctcttgc gcaaagcggc ccgatttcgc atcctcgccg tgaagccggc aacgtatgta
     8821 ccgcgaattt tttgggggcc agggttagcc gtaatggtga tcgcgtgccc gatgaggaag
     8881 gtttaggggc tatagcagac gagttctcga gcctagatcg tctgggaaaa tggtgaagac
     8941 gatggaaaag atttcagtag ctgaggcacg acgcgaagac gccattgggc gcgaagtcct
     9001 tttgcaggga tgggtccgaa cacggcgtga ttcaaaaggt ggtttcagct tcttggagat
     9061 caatgatggt tcgtgtctcg gaaacatcca agtagtggcc gacgggacgt tgcctaacta
     9121 cgaatcggag gtaaaacgcc tgacaaccgg ctgcagcgtc gccgtggagg gagtcgtgcg
     9181 ggcgtctccc ggtcgtgaac agcccacgga agtttacgca acccgcgtga ccgtttatgg
     9241 ctgggccgat ccggagacct atccccttca gaaaaaacgc cattcttacg aattcttgcg
     9301 gacaattgcc catttgcgcc cccggaccaa ttccttcgga gcggtagcgc gggtccgcaa
     9361 cgccgtatgc ttttcgatcc atcgtttttt ccaggaggaa ggtttccttt acatccacac
     9421 gcccatcatc acggcctcgg actgcgaagg ggccggggcc atgtttcgag tgacgacgct
     9481 cgacccggcg actcaacaac ggctcgatga cgctgcggat ttctttgggc gacgtacata
     9541 tttaaccgtc agtggacagt tggaagccga ggcctacgcc tgcgcggtcg gaaaggttta
     9601 tacgtttggt cccacatttc gcgccgaaaa ctcgaatacc acccgacatc tcgccgagtt
     9661 ttggatggta gagccggaag cggccttttt cgaccttgac gataatatga acttggccga
     9721 gcggttcctc aaacggatct ttcgggatgt cctggaacaa tgtcccgagg acatggagtt
     9781 tttcaatcgc tgggtggata acacggtgat tgaaacgctc aataagatca tcgagagcga
     9841 attcgtgcgg ttgacctata cggaggccat cgagattctc aaaaaatccg gcgaaacgtt
     9901 tgaattccct gtcgagtggg gtcgcgatct ccaggcggaa cacgaacggt ttttgacaga
     9961 aaagcacttt cggcggcccg tcatcctcac cgattatccc cgccagatca agccttttta
    10021 catgcggcta aatgacgacg aaaaaacagt ccgggccatg gatgtgcttg tgccccgtgt
    10081 gggggaaatc attggtggaa gccagcggga ggagcggctt gatgtcctcg aacgccgcat
    10141 ggaagagatg ggcctcaata agcaaaacta ttggtggtac atcgacttgc gtcgatttgg
    10201 taccgtgccc catagcgggt tcggcctagg cttggaaagg gcactccagt tcatcacggg
    10261 gatgggcaat attcgcgacg tgattccgtt cccccggacg ccgggcagtg cggaatttta
    10321 gcaggaaaaa gggcgtaaaa cggcttcctc gccggggagt taccgattcg caagagtgat
    10381 cattcatgcc tgattggacg tttccatcca tctttcgtct ccgccaaaag tttccctctc
    10441 atcgcatcga cgatattccg gcccgaatcg tggccgagtt tgaacgtgcc aacgttcgga
    10501 acagggtccg tccggggcag acagtggcca ttacggcggg cagccgcggc atcgccaata
    10561 tagtccttat ccttcgcaca gtcgtggaga ttcttcacca gtggggggcc cgacctttca
    10621 tcgtccctgc catgggtagt cacgggggtg gtatggtgga agggcaaagg ggtgtcctgc
    10681 atacctacgg gattaccgag gattcggtgg ggtgtcccat tcgtgctacg atggacaccg
    10741 tcgtcgtagg acgggccccg gagggatttg acgtccattt cgaccgtttt gcttatgagg
    10801 ccgatcatgt ggtggtcgtc aatcgcatca agccccatac cctttttgtc ggtccgattg
    10861 agagtggcct gatgaaaatg cttctcattg ggttgggaaa acaccgcggg gccgaaattt
    10921 accaccgtgc tgcgtgggat tattcctttg atcgcatcgt acgggacgtc ggacggattg
    10981 ttctttcgcg atgccggatt cttttgggac tggccatcgt ggaagatgcc tacgatgcca
    11041 cggcccattt ggaggctgtc ctgcccgaag cttttgagac ccgcgaacca gagcttttga
    11101 ggctcgccaa agagtggatg ccacggctac catttgacca tgcccacgtt cttcttattg
    11161 atcaattggg aaaagaccta agtggttccg gaatcgatac caacattatc ggccgaaagt
    11221 tcaacgacca tcgtgcggct ccgggtgaaa agcccgatat ccgcttcatc tgtgttcgcg
    11281 ggttgagtcc ggggacacat ggaaacgcaa tcggtttggg tcttgcggag ttttgccgga
    11341 gtgacatact cccacaaatt gacgtccatg ctacgaggat taacgccatc acatctggtc
    11401 atgtttccgc ggctatggtc ccgctcgatt acccgacaga ccgggaaatg ttggggcagg
    11461 ccctggcgaa tatcggcatg acgccaccag aacacgcccg attgatgtgg attgccgaca
    11521 cgctgcatct caccgaagtc gaatgctctg cggcctacct ggccgaggct aaagcccgag
    11581 atgacctgga aatcttgacg ggggttcggc ctatgccatt tggcgatgat gggaacttgc
    11641 cggatatgat gaccctgcga gcttccagct aacagcctat cccatagtct cccctcgccc
    11701 gtttggcggg agaggggtcg ggggtgaggg tggctcgaat cgctgccggt tttcttaatc
    11761 gattgcgacg ttcgcttgcc ctcaccccaa ccctctccca gagggagagg gggcttcttg
    11821 agatggctct gagcagttga aacaatcacg ggaagacgca ttcgatacaa aagaattgcc
    11881 cttatcttgt cgtatctttg gcttatccgg aagaaaacgt tattcgtgcg agagaattga
    11941 gggaacggcg gatttcttcg aaaagaagaa aggtggactt tatgagccga taggggccga
    12001 gctggcttcc gcatggcttt tcagcaagga agcgagacgt ctcgattgac catccgccca
    12061 tcagtttgtg gtggtcttcc agccacaagg agcacgatct tgaaccgtag gctgtccgcg
    12121 atcctgccgt tccgtaatgc cgagcatgtg gtcttgaatc tgattcccag ttacgtggat
    12181 gtgttctcgg agttgtccag tgactgggaa atcctggcgg tggatgatgg ctcggaggat
    12241 agcacgcccg agatcttgtg tgaatttgcc caaatgtatc cccaagttcg ggtagcaatt
    12301 catggccagg cccgcggatt ctatgcctct ttgtgttctg gtctgtcgct tagtgtaggg
    12361 aatatcttgt ttctgccgct gcggtaccaa ccgatgacgc tcgactcgct ccaccgtctc
    12421 tggtcggcag tgcagaacca ctcccttgtg atcggattgg ctgccaatga attcggccaa
    12481 gatgagatcg aatgtctcat gctccagcgg cgggtggcgt cgccgattct gcccgcgttg
    12541 tcgtccacag aaagactggc tgcggtggtc aaccggtttg aatcttctgc ggtgatcgtg
    12601 ccgctgcgac gggtggcatt ttcgcaggag aagccttacg ccagttatgg acgaaccttt
    12661 accacaacca acgggcggga agcgatgcca cgtgctccca aatccttgac ccttcaggct
    12721 gctaaggccg ttttcagccg catcaagcag tttgccctgg atgagtgact tggggatccg
    12781 cctcctcaca cgtcaaaaat gacctgagcc gtaaactcat tggcttcctt ctcgattctc
    12841 agcccgtgat aggtcacggc cttgatttcg acttccccac catctcggac cggatcgtac
    12901 gcgcggacct tcagtcgtgc tttaagaccc attgagtcaa actgtatctg ataatccacg
    12961 ggaataaggc gttccactga gaatagatag agaattgttg acagccaatc gtggagaagg
    13021 tcttcccaag tgggggacag tacgatccgt gagccattgt cggatgacgt ttcgcaagga
    13081 cttggctggg gttctgggat aggaagatcc tcggccggaa tggtcacctc cctcaattcc
    13141 gtcggtgtag cccgtaacgc ttcggttggc ggtgacgcag cctgagcgga aggagtcgct
    13201 ccagttgggg cgctggtcgt gtcttccaac tgtggccccc ggcacatgag ctgaatcatg
    13261 gcctctgccg cttccttgaa gagtccttca aggctcggtg ctcgtaccct gatgccgaga
    13321 tcagcggtgt gctcaaaaac ttcaaacatc aagctctcct ccttttcccg acaatttgag
    13381 ggacgtcgct tgacatatcc tataccccga accgtcttcg gagaattggt tgcacggggt
    13441 gccttgccaa tccgagggtt aaaagtcatt ggagtacaca gcggtgcacg ggccgttcga
    13501 tccggccacg acagcgtggc tctgcatctc agcagaaggc ccctctccgg aattcgcctt
    13561 gacaaaagcc ggaaaatcag cacaatcccc gtcagacttg accacacgga agtggtcatc
    13621 aattgtaacg tcgttttccc acttgatggg cgagcattgt aggtttgtcc gtaatcatcc
    13681 gaggtacgga ggcctcggcc gatgaagtta tgctccagag cgggcacaac gatcacgctc
    13741 cagcagctcc tgccggacct tcaggtgttt ggcgacggag agttgcaggt aggggtgtgc
    13801 catagccagc cgagccggat caagccgggt gatctgtttg ttcttctgca cccgcccgga
    13861 cctcgccggg acgccgctat tgagcaggcg gtggggcgtg gatgtgccgc catcctctcg
    13921 gaagacgtcc tgccgggttt atctgtcccg gtgatctgcg tgtccgacgt tcgcacggct
    13981 tacgggcgga tctgccacgc ccttcacggc tttcctaccc aaaagctggg tgtgatcggc
    14041 gtgacgggga tgtacggaaa aacgacaacc tcatgccttg tggcccgcat cctggtgcaa
    14101 gccggttatt ctgtgggaat gctcacgagc ctgggctatt tcgaagggga agaatcgacc
    14161 tttcctgtgg agacgacgcc gcccccagac gagttagccg caaggcttgc ccggatggcc
    14221 gacaacgaat gcacgcacgc cgttgtggag gtttctgcca aggccatcaa gcagcgatac
    14281 actgccggtc tggaatttga caccgtggtg tttcttaatg atgaggcaga gggtgcctct
    14341 tcacacgatg cccgatgtcg gtggttttcg ttggtcgata acctacgacc ggaagggctg
    14401 gccattttta acgcggacga ttcacgttgt cgggcggccc tggccgaaac ggaccatccc
    14461 gcattgactg tgggtattca cgcggccgcc gaaatcaagg ctcgggaaat tcaccgacat
    14521 cttggcgagg aaacctttta catctgtgtg ggggacgata cgtttccggt ccgcacaaag
    14581 atgttcgggc gccaccatgt tcggaactgc cttgccgcgg tggctgtggg gttaacgtac
    14641 cacgtcccca tggaggtgat cgtccaggct atcgagagtg tggatcagat tcccgggcgg
    14701 cttcagccgg tcctcggtgg tcaaccgttc tctgtgtttg tcgatgctgc ggaaacgtcc
    14761 acgtctttag ccgcggtgtt agaagcggtt gtgccccagg ttcagggacg tgttatttgt
    14821 gtggcgtcgc tgccgcgcaa tccacggcaa gaacttggaa agtggttgga gttgatcgac
    14881 gcgcattgcg gactgggcgt tatcaccctc gccaatcccg gacgcgaaga cccagcaatt
    14941 ctatcaccgt tggcctacga atctatgaaa tcgccccggc ggattgtcat accggacaga
    15001 acggaggcca ttggttgggc cttgacagag gctcgcgagg gggattgtgt cttggtcatc
    15061 ggtaatggac accgccgatg gcgccccttg aaagagaacg atttagaagt ggacgacttc
    15121 cagttcatcc gccagtggtt ggagcaggaa ttcccccatg taacgtgaat agggcttgtt
    15181 aaaaggcttt ctcagacggt gctgggaggc cacagagaaa agtcgagcaa ggaggtttgg
    15241 aagatacaca gcacgacatc cattgcaagc cagtgacaac cccatcgatt gcgttaggtt
    15301 tgcgggagcc tgatgcagcc ctccctcctg gggtgaagca tgaagcttgt cggatgatgc
    15361 ggtttctggg tcggccgctc cgacgagcgc ttctcgatgg ggttcggtga cctgagggcc
    15421 ggagcggtgc gggtcacacc gctccggtca cctcagggag aaggactcat cggcagtcga
    15481 tggcgattcg tcaccttcgc ggaatgactc ttcggtcctc agtcttggta gcccaaatga
    15541 tctccggacg agttattttg gtcttctgcc cggatttttg cccgggcctt tgctgacgga
    15601 aggcttcttg ggacctggct tcttctgatg tttggcagcc ggtgggacgg tgcctttttt
    15661 ggcaaacgga cccggttgag gtgattccac aactcctgtg atcatttctt cgcaagacag
    15721 cttgcggaaa cggcattcac gcggcgggat gagccgttcc tcgggacaga tctggagaag
    15781 gacgctttcg gctccggttc ccgggatcgt gtatcggtca accacccgca gggccaaccc
    15841 gtgcatcagg ccatagtgcc gtgcttggcc gcgctcggtg acccaacggg ggcctttgag
    15901 gatcaacatc cgatcaaaac aatcccagta aggacgaaac cagcgaagta gattcagaag
    15961 tggggccacc gcacgaataa cgagggaatg gaactcccat ccgcctgctt ccaggtattt
    16021 ctcggcccga ccaatgagca tcgggatttt cagtcctaaa cgagacacaa tatccgcgac
    16081 ggcacgagca cgtttgccga cggtctcgct aaggacaaca cacaagtctg ggcgaagaat
    16141 cgacaaaacc acgccgggga caccactgcc cgtcccaacg tctaggatca cttcatcagg
    16201 ctggaggcat cgcatgaacg ataaggaatc caccacgtcc cgggcaacga actgatcgta
    16261 ggtatcatgg cgggtaaggt tcatcttctg attccattcc cgccgaagct gaaaatattg
    16321 atcgagaagc cgcacctgct ccgggaggag cgcgatttgg cacttggcca aggcctcttc
    16381 cagtgtggcc gaagtaggtg cccctggggc ggagggttga atgtcagaat cttcttggta
    16441 gtcggacatg cgaaatcaaa atcggtcacg caaacggggt aagtaatgct tggactcgga
    16501 cgacagagga agacggggaa tctgtcacgt cgtaccgtac agctttttga tcacccaagc
    16561 catgttttct ccgagggttt gcatggtgcg gataccttcc tcgtcactac tgacctcgcc
    16621 cggttgcaga ccgcggccca cattccaata gctggaacct acagtaatca tttgagcgat
    16681 ggagaagtaa tgattgagcg agtcgaacgt atgaatggcg cccgcccgtc tcacggccac
    16741 gatcgcggcg cccacctttc tgcgaaaaag atcgccagcc gcgcgggcga ccatccctga
    16801 ccgttccatc agggccttca tattggttgt cacatcagcg aaatagacag gcgacgccaa
    16861 aatgacacct tgagcctcgg tgattttctt gatgtaatca ttgacagcgt cgtccttttg
    16921 atggcattgt cccttttctt taaaacaacg ataacaggct acgcagccgt ggaggtgttg
    16981 ccctgccagt gaaatgtgct ccgtctcgat cccttctcgg cggaggacct ggagggtcgt
    17041 ctccacgaga atggcggtgt taccatcttt acgagggcta ccgttgaatg ccacaacctt
    17101 catgttgggg tcctttcacg cttgctggaa cggagtcgtc tgtcttgttt gtgcaaacga
    17161 actgcccctg cccgggcaga agaacgcatg attactggtg cgacttcaga ttgtcttttg
    17221 gtcttgacca atacttcgat tgtatcgcaa tcggtgattt ctcaaatggg attcccacct
    17281 gatgaaactt ctgcaatggg tggcaggcaa ggagttcgtg aataaaatcc cgtacgtgag
    17341 gggagcaacc ttgataaacc caacgtcgcg accacggtgc gatgaattag tcgcgaattg
    17401 tctttttcgg ccacaagggc acgtggcctt ccgtgacgag ggcgtccatt gccaggggca
    17461 gtcgttgtgg ttgcgctttt tctcgaaccg gaggattctg cgaagagcct atcccataat
    17521 cttccctgac ccgttcggcg ggagaggggt cggggggagc acgctcgaat cactgccggt
    17581 tttctcaatc gattccggcg atcgcttgcc ctcaccccaa ccctctccca gagggagagg
    17641 ggggtttttg ggataggctt taaaccatgt tacgctgtct cgtctgccga atgacgacgg
    17701 atcattcttc acggcgcttc actaaaagac ctcattttgg agggcctaag cggatatact
    17761 gaagtaggtt gggccaatca acaaacagcc agcaagtctt ctttccccaa tcgcatctat
    17821 gccagacgaa ccgacgatcc cacaaggtgg tgccgaacgc cgactatcac aggcgatgag
    17881 cctgcaaggt cgccgtccgc ccgccgatgt gccgggctac gatcccgagc gcctcttagg
    17941 agcaggggcg tacggggaag tttgggtagc cctggagcgc aacacgggcc gtcgggttgc
    18001 catcaagttt tactcccacc gcggcgggct cgattggtcc ctgctttccc gcgaggtcga
    18061 aaagctggcc ttcctctgcg ccgaccggta cgttgttcag ctcatcgccg tggggtggga
    18121 ctccgagcct ccctactacg tgatggagtt catggaacag gggtcgttgg cggaacgtct
    18181 ggcaaagggg ccgcttcccg cgaccgaggc cgttcgcttg ttccgcgagg tcgctatagg
    18241 attgcttcat gcccataaca aaggggtctt acactgcgac ttgaagccgg gaaatgtcct
    18301 cttggatcag gacgggaagc cgcgtctggc cgattttgga caatcgcggc tgtcgcatga
    18361 gcaactcccg gccctcggta cgcttttcta tatggcgccc gagcaggccg acttgaatgc
    18421 ggtgcctgat gctcgatggg atgtctatgc cctaggggcc ttgttttatt gcatgctgac
    18481 gggccatccg ccccaccggg atcaggagac ggccaaaaag atcgaggaga tcaccgatct
    18541 cgaggagcga ttggcggcct accggcgact gatcgaggaa tcggggattc ccacggacca
    18601 ccgttcgatt ccgggtgttg atcgtggact ggcggcgatc ttggaacgtt gcctggcccc
    18661 agacccaaat cagagattcc caaacattca gtcggtcttg gtggccctcg aaacttgggc
    18721 ggccgagcgg gcacgccggc cgttggtggt tctcggtttc gtaggacctg ccttgctcct
    18781 attggtcttt gccctttttg cctggcgggg atttgccaca accgtcaatc gatcacgcga
    18841 tatgctgatt catcgggcat tggagaataa tcgcttgatg gcacagtatg tggccgacct
    18901 agcaggaaag gagttgcgac gccggtttga ggcggtggag cagttggggg gtaatgctcg
    18961 attccaggat ttgatccgcc aggtcatccg cgatccggaa tatgaggaat tgtcgcgaga
    19021 gctatctcgt gccgatttgc cggaggaaga agccgaggtc ctgcgtcggc ggtttcgcag
    19081 ccatccccta cgggcagccc tgcaacggga gttttcggcc cttatcccgc cgtggatgcg
    19141 acccgaggaa tcaggggaat catcggttga cgatgtggca agttggttct tctgtgatcc
    19201 gaacggcatc tccacggcgc gtgttcccga atctgtgacg attggccgga attatgcttg
    19261 gcggagtttc ttcacgggct tggatcgaga catgccacgt gagtggcgcc ccgcacaagg
    19321 agagcacctt cgttccccca agctctcggc ggtattccgg agcgaagcga cgggattgtg
    19381 gattgtggct gtggcggccc cggtgattga cttgcggaca aacgagtttt tgggagtggt
    19441 ggctttgact gtccgcgtga cccggttcgt ggaattggaa ggcacacccg cccaatttcc
    19501 ggttttggtg gaactgcgac cgggagacca caccgggatg attcttcaac atccgctctt
    19561 tgatcaggtt cttttacggg aacaacgact tcctgagcga tttcagaatt atcgcctttc
    19621 taaagatgcc cttccaccgg atctggacaa aggggcgatt catgatccgc gagcgatcgg
    19681 ctatcgggac ccgctggccg ccgaccccga aggatcactt tttgcacgcg attatttggc
    19741 tcaggccgcg ccggtgagac tctcttcagg gaaagactca caagacacag gctggatggt
    19801 catcgtgcaa gaggattacg atgtggcggt ccgcggtccg ctggaaggct ttcagaaggc
    19861 gctggttcgc tacggcctga tttcggccag tctggtggct gcaatcctcc tggggttgtg
    19921 gacactgacg gtgcgccttc tgaacgaaat tcacgaaaat cacctcttga gtcgttctga
    19981 atatcttttg cgtgaaacgt cgcgaggatc gcaccgcgtc gttccgaggg tcatcacgga
    20041 tcacccgacg agtaccgcgc cctgggtgtc tgattccata cctagtggac cgagtattga
    20101 a
//
LOCUS       DAOSUT010000075        19694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1773_length_19694_cov_9.747747, whole genome shotgun sequence.
ACCESSION   DAOSUT010000075 DAOSUT010000000
VERSION     DAOSUT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1773_length_19694_cov_9.747747"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="mnmG"
                     /locus_tag="PLD05_08560"
     CDS             complement(<1..1334)
                     /gene="mnmG"
                     /locus_tag="PLD05_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOP77530.1"
                     /translation="MTSVYDFDVIVVGAGHAGVEAALAAARMGANTALLTSNLDTIAK
                     MSCNPAIGGVAKGQIVREIDALGGAMGQAIDATAIQYRLLNRRKGPAMHGPRAQADKV
                     AYHQKVKQICESQPNLALRQETVESLLVEKTASGLRIVGVAVRSDAIYRAPCVILCTG
                     TFMRGLLHYGEKKMEGGRAGEEAVLGLSQSLRELGFTVLRFKTGTPPRLNGRTIDYSR
                     LEMQPGDENPVPFSFLTEKVTCEQVPCWITYTNERVHEIIRANLHRAPMFTGQIQSVG
                     PRYCPSVEIKIVRFPDRPRHQLFLEPEGRSTHEVYVNGLATSVPRDVQEAMIREIRGL
                     ERAEIMRYGYAIEYDYLPPDQLQLSLETKRVAGLFLAGQINGTTGYEEAAGQGLMAGI
                     NAVRKLFGKDPVVLARHQAYIGVMIDDLVTRGVDEPYRMFTSRAEYRLRLRH"
     gene            complement(1361..2155)
                     /locus_tag="PLD05_08565"
     CDS             complement(1361..2155)
                     /locus_tag="PLD05_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOP77531.1"
                     /translation="MDSLLDALQEGRLIELPEDDDKEEALRILARILEAIPSLPAGLD
                     IEGLVLERERTSYTSLGQGWACPHARVSFEGDLLCVVGWSAHGITYSPDDKERVSIII
                     MHLVPENQRNQYLREISLLAKAIQSDPKWREIPSAKELTEVRDRLLDLISSAKETVGP
                     DARARMIRLQRPKVEPPPITDLSNLVIEPVTLVVGPDFRPIVLTQHAGLAKYLETANG
                     VVEKIETEGVFQNGGWRIIRRSKMVFQQGRVSYDCIAITTARKTPS"
     gene            complement(2158..4821)
                     /gene="mgtA"
                     /locus_tag="PLD05_08570"
     CDS             complement(2158..4821)
                     /gene="mgtA"
                     /locus_tag="PLD05_08570"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOP77532.1"
                     /translation="MLRSLPRNMIEALRTRSKEETRVAQALAPHEKLLIEICTLTPGE
                     ALARLATKWSGLDEADVEARLDEYGHNELPHAQRLSFWADILHRCRNPLNIQLFVIGG
                     VSGLIGEWKSAIVVAAMVLLSIGLSYLLDRRSNRAVEALGKRVQPRSLVLRGGREVEV
                     KISEIVPGDIVLLHAGAMVPADVRLLSAKDFFVSQSVLTGESMPVEKTVFLNGSAATS
                     PWDLPNACFLGSNVISGTARAVVVNTGTRTMLGSISARLIETRPETSFDQGIRGFTWL
                     MIRFMVVMVALVFLIVGFTKGNWVEALLFGLSVAVGLTPEMLPMIVTVNLAKGALSMA
                     KRKVIVKHLPAIQNFGAMDVLCTDKTGTLTQDRVVLERHVDITGRPSDDVLTYAYLNS
                     YFQTGLRSLIDHAVLDRANLDVESSQCRLVDELPFDFQRRRMSVVVDYEGDHVLICKG
                     AVEEIYECCRYYQIGDEIYPLIPVIQNDLFEEVERLNREGFRVLGVAYREFPRDKTTF
                     SVADESDLILLGYIAFYDPPKDSALEAIRLLKEVGVTVKVLTGDNALVTEKVCHDVGL
                     EVRRIVSGNELADLTEEEFDRVVREVDVFVKLSPSQKEQIVRKLREAGQVVGFLGDGI
                     NDAAAMKAADVGISVDSAVDVAKEAADIVLLEKNLLVLEEGVMEGRRIFANIVKYIRM
                     GASSNFGNMFSVLGASYLLPFLPMKPIHILVNNLLYDVSQTGIPSDNVDAEQVSRPLK
                     WDIDNIRRFMVFIGPISSVFDYLTFALMWFIFGCSAYLQGGTDREYWMSLFQTGWFVE
                     SLLTQTLIVHIIRTQRIPFIGSCASAPMILTTLAVMAVGIWLPYSPLADVLGMVPLPA
                     TYWLWIGSFLLAYGTLTHLVKCWFFRRYGDK"
     gene            5609..6520
                     /locus_tag="PLD05_08575"
     CDS             5609..6520
                     /locus_tag="PLD05_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77533.1"
                     /translation="MTTVLVVDDSPIDSQLVGSLLKKRFDWKIEYAESGTEALARMKQ
                     NVPDLVITDISMPEMDGLELVQHIRKMFPEVPVILTTAYGTDMLAIEALERGAASYVP
                     KSQLAARLAETADSVLRIAEQNRERERLFRCLEYIEFEYVLDNEAELIQPLVSVVQQT
                     LAGMEFADFAGRLQLGIALKEALLNALFHGNLEISKSEIEAVSDHLLEDSELSLVAQR
                     RQDPRYRDRKIYVLVRITKEEARFVVRDDGPGFDVHSVVRKKDESPELEAQRGLSLIQ
                     TFMDEVNFNEKGNEITLIKRREKLSKA"
     gene            6582..7187
                     /locus_tag="PLD05_08580"
     CDS             6582..7187
                     /locus_tag="PLD05_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOP77534.1"
                     /translation="MFPHLRVLLVDHHDSFVYNLGRYFELLGCRIAIVRPTEVPESSN
                     ILRLFDLVVLSPGPCGPQNVVTSVELVHNLADQMPILGVCLGHQIIAEAFGGRIIRAA
                     EPVHGRATPVWHDGQHEFAGIPNPFPAGRYHSLIADRDSISEEVEISAWTDDGIPMAI
                     RHSRYPVVGWQFHPESVLTPLGLDLLRNLLLSWNQSLRFAR"
     gene            complement(7230..8120)
                     /gene="dapA"
                     /locus_tag="PLD05_08585"
     CDS             complement(7230..8120)
                     /gene="dapA"
                     /locus_tag="PLD05_08585"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOP77535.1"
                     /translation="MTTRAEQFAGLSVALITPFRDGQVDYARLEEQVEFQIQAGVTCL
                     CPVGTTGESPTLSHPEHREVISAVIKAANGRVKVMPGTGSNSTEEALELTRFAAQVGA
                     DAVLVVSPYYNRPPQEGLYQHFKSLAQAVDIPICIYNIPGRTGRNIEPETFFRLAEFP
                     NIAMVKEASGSLDQVSQLTTQTDLTVLSGDDSLTLPILSVGGRGVISVIGNIVPQDMI
                     AMIRAFEEGNIPEAIRWHKKLFPLARDMLGLSTNPIPIKAAMRLLGRDSGELRLPLTP
                     LSSDQENRLRKVLADYGLLQ"
     gene            complement(8186..8926)
                     /locus_tag="PLD05_08590"
     CDS             complement(8186..8926)
                     /locus_tag="PLD05_08590"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOP77536.1"
                     /translation="MIHLGVNVDHIATLRQARRTYEPDPVWAAALAELGGADGITIHL
                     REDRRHIQDRDLRLLRETTAVKLNLEMACSEEIVAIALAVKPDQATLVPERREEITTE
                     GGLDVAGQLARVTDFVKRLQDAGISVSLFIDPDLAQIDAATKTGAVAVELHTGQYANS
                     SGKVQQQELQQLRKASHEVLQRGLKLHAGHGLTYRNVQPVAAIPGMEELNIGHSIVAR
                     AVLVGMVQAVREMKDLMVWAASHPEHFH"
     gene            complement(9214..10191)
                     /locus_tag="PLD05_08595"
     CDS             complement(9214..10191)
                     /locus_tag="PLD05_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP77537.1"
                     /translation="MTRKYVNQLRPEETVNEVFQVTSRQLRPNRNGILYLQMRLADRT
                     GSLDARMWNVDETILNRFDAGDYVRVEGTTQIFQGAIQLIVSRLQRVPAAEVDPSDFS
                     LVNTLEIDRAMNQLAERLRGINNPHLRALIDSFLLDEEFMAKLAESPAAVSFHHAYRG
                     GLAIHIANMLEMAASIAPLYPLLDKDLLLAGVFLHDIGKINEFAGGSELTYTDSGQLL
                     GHITIGLELLAEAVDKVEKLLGETFPEELLLRLKHMLVSHHGEYEFGSVRLPMTLEAM
                     ALHCLDYLDSRMAACVERIQGDPPFDNTWTAFFPALNRKLYRGTNNGGV"
     gene            complement(10248..12647)
                     /locus_tag="PLD05_08600"
     CDS             complement(10248..12647)
                     /locus_tag="PLD05_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOP77538.1"
                     /translation="MRVATATVMLTTWFLWTSAPLRGTTASEPLTHQPPALRIQLSVT
                     PSVYAFDLRDVRVTAGPFRHAMEMDKRYLLSLDPDRLLHVFRSNAGLPSNVQPLGGWE
                     DPKCELRGHFVGHYLSACSLMFASTGDQSLRERVAYMVAELAKCQERLGGGYLSAFPE
                     EFFDRVETTGRVWAPYYTLHKILAGLLDAYVYCENDQALEVAKKLGDWVIARNQRLSD
                     QQMQRMLGVEHGGMNEALANLYALTGEAKYLQIAERFNHLAVMEPLSRGTDVLTGLHA
                     NTQIPKFIGAARLFEVTGKQWYHDAAVNFWKFVVNERSYVIGGHSDNEHFSPKERLSE
                     ALGPNTTETCNTYNMLKLTRHLFCWKPLAQYADYYERALYNHILASQDPATGMMCYYV
                     PLRSGSHKVYSTPLDSFWCCTGTGVENHAKYGDSIYFHDGKGALFVNLFIPSELQWSQ
                     TGLKLRQETNFPYEPRSTLVITNPPSSDVTIYIRRPFWTTDGFQISVNGEVVTESPTQ
                     EGFVPLRRTWKAGDRIVVDLPMTLRTEAFRDNPHRLAFLFGPVVLAAELPKMSQQQVR
                     KLPPMGSYNPEIPVIVSETIDLGRFKPVEGKPCVWQSPDGLFRSLAEDQAVPVRLEPF
                     FAIHEGRRYVVYWDRVSHQAWKEMIANYQEELVKWRELEKKTIDHVIIGNPESEKQHN
                     LQGENTSHGTFQGRAWRHATNGGWFAYDLQLADKPRLALLVEYWGSDSGNRVFDILVN
                     GQTIATQRLANNRPGHFYRETYPLPDNVLQNGKQVTVRFQAHPGATAGGIFDLRLITL
                     E"
     gene            complement(12833..13801)
                     /locus_tag="PLD05_08605"
     CDS             complement(12833..13801)
                     /locus_tag="PLD05_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOP77539.1"
                     /translation="MRGFSLSWIVAVAVVICPSWAWAGNQEVAQEIASALKNSGQLRG
                     YKIGVRYQDGIAWLRGSVTDEQQKLAAEKVVSRVPGVKQVVNELAVVPAGATDSPNLQ
                     TAAGAIAPERTIASSSAPSATAQPAAGEVAQRPVQLADPVPSVFAPEPVRPTAAISAM
                     EGPTLAIPELPQADMPMATQTSATVAPEAAPRPIRPLPIAMTQEEVSREAAPAPAAQP
                     LGRPLPMSVGAPGAAAPARFDQPNMPNYAWPSYAAYPNYAAVTYPKQYSATAWPYIGP
                     FYPYPQVPLGWRKVTLEWHDGWWYLDFDDGASKGPFSGLFRAIKGH"
     gene            13800..14207
                     /locus_tag="PLD05_08610"
     CDS             13800..14207
                     /locus_tag="PLD05_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77540.1"
                     /translation="MPVLLFGIHVPTVGALFWKADFLSRLDQLPEKPNLIFTGRHRQN
                     VAYNRKMVTAISEKPQAILPIYDSTILNTERYVFSVFGKVVELFPHSVSSNFPASVIF
                     VKCSKKPGLNRRMISIGTESPLAPVAFPMRPGD"
     gene            complement(14397..14657)
                     /locus_tag="PLD05_08615"
     CDS             complement(14397..14657)
                     /locus_tag="PLD05_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77541.1"
                     /translation="MIHLVAAILLVSVQFLGCAMAIATRFPGTRDWASTGFHGILALL
                     AGIALAACGQSHHVWGSCLVTVSVMILLSMADFTQPTDRKSV"
     gene            14995..16482
                     /gene="pap"
                     /locus_tag="PLD05_08620"
     CDS             14995..16482
                     /gene="pap"
                     /locus_tag="PLD05_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOP77542.1"
                     /translation="MLELVDLNQRISGEEYEKIFPDLEAQLAACQRMARQSGIPIAIV
                     FEGWDAAGKGMMINRLAQVLDPRGFRVYLTGDPSAEEQRRPWMWRFWKELPGAGEIVM
                     FDHSWYRRILDDVAQGRLSRKEYQQAVHDILEFEKQLADSGLLLIKFWLHISAKEQRR
                     RLKRLEQSPATRWKVTKSQWEQNRKYKKWQKLVETALQRTSSSQNPWTVVEANQGKFA
                     RVKVFRTIISAIKETCARREREPAPSLAPMPEPDESQFYRPTILDRVDLTKSIGREEY
                     ERELEVLHKKLYELEHRCYLAKIPVVIVYEGWDAAGKGGTIKRLTRALDPRGYEVVPV
                     GPPTTEEKAHHYLWRFWRRIPKTGHITIFDRSWYGRVLVERVEGYCSQEEWERAYREI
                     NEFERQLADFGTVIIKFWLHIDSDEQLRRFERRQSDPQKQWKITEGDWRNRAKRKHYE
                     LAVIDMLQRTSTLYAPWTILEANCKLFARIKSLRVVVERLEAATR"
     gene            16743..18098
                     /locus_tag="PLD05_08625"
     CDS             16743..18098
                     /locus_tag="PLD05_08625"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HOP77543.1"
                     /translation="MLEIVASLLLVLGVAESKVNEPNPCDVTKKNPRVVQVFPIGLAW
                     SSHPVSFCLYTSPPYQYVGYYDAERRLVIAARELSSNRWEIQRLPTSVGWDSHNSIVI
                     AVDRDGYVHVAGNMHVTPLIYFRTEQPHAISTFQRFPMVGQDEQRVTYPRFLFTANGD
                     LLFFYRDGASGKGRQFINRYDVATKSWSRLLQTPLLDGGEEMSAYPSGPDLGPDGRWH
                     MCWVWRDTPDCATNHDISYARSSDLLHWETIDGKPIELPITPRTPGVVVDPVPVKKGL
                     INVAHYVGFDTQHRPIVTYHKYDEQGYSQIYNSRWENGRWVIRQATRWEYRWEFSGGG
                     TIQVEISSSRIHLLPDGRLAQRFSHARYGSGIWIFDPASLSVVATCPAPREIPSELAE
                     LESDFPGMEVRFARDAGKPPAGEEYVLRWETLAANRDRPRAGPLPPPTTLRLYRLQRD
                     P"
     gene            complement(18217..>18495)
                     /locus_tag="PLD05_08630"
     CDS             complement(18217..>18495)
                     /locus_tag="PLD05_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77544.1"
                     /translation="ATALLLSLLTLESRSLTILAIVLATWAITTGAITTLGLGLVAEL
                     LERARTNRHPAYIVRYMSGIEHESAVIPLSRENFRSIPSHAPVKRTGT"
     assembly_gap    18496..18595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18596..19089)
                     /locus_tag="PLD05_08635"
     CDS             complement(<18596..19089)
                     /locus_tag="PLD05_08635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP77545.1"
                     /translation="MDGSPESSNFPPATTTISVVIPVHNEVGNVAALQKELVPVLESL
                     SMPWEICFVDDGSTDGTSLLLDQISHTFPNTRVIHLTRKFGQSAAIQAGLDHTHGEIV
                     VTMDGDLQNDPHDIPHLIETLQKGYDIVHGWRKHRHDHWLTRRVPSLMANWLIRKITG
                     VEIP"
     gene            complement(19362..>19694)
                     /locus_tag="PLD05_08640"
     CDS             complement(19362..>19694)
                     /locus_tag="PLD05_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77546.1"
                     /translation="YGSPGDDSLTVDPTFARLKGSGFYNRAKFFERIEVYGGGGYDTG
                     VVYDSAGADHLYATSVEALLQNPDLPYYVYLTQMEKVKVRGNNPPNTKHIEGIVDWLI
                     ATGFWTDI"
BASE COUNT         4545 a         5338 c         5347 g         4364 t
ORIGIN      
        1 tcatgacgca atcgcagtcg atactcggct cgacttgtga acatccgata aggttcgtca
       61 acaccgcggg tcacgaggtc gtcgatcatc acaccgatgt aggcctgatg tcgcgctagc
      121 acgacagggt ctttgccgaa cagctttcgc acggcgttga taccggccat gagcccttgt
      181 cctgccgcct cctcatagcc tgtggtcccg ttaatttgac ccgccaaaaa caggccggcg
      241 actcgctttg tctcaagcga gagctgcaat tggtcgggcg gcagatagtc gtattcgatg
      301 gcgtaaccgt aacgcatgat ctctgcccgt tccaacccgc gtatttcgcg gatcatggcc
      361 tcttgcacgt cgcgcggcac actcgttgct aatccattca catagacttc gtgcgtcgat
      421 cgcccttcgg gttccaggaa aagttgatga cgtggccgat cgggaaagcg gacaattttg
      481 atttccacgg aaggacaata gcgcgggccc acagactgaa tttgtcccgt gaacatcggg
      541 gcccgatgaa ggttcgcgcg aatgatctcg tggacccgct cgtttgtgta tgtgatccaa
      601 cacggtacct gctcgcacgt gactttctcg gtcaggaacg agaaaggcac gggattctcg
      661 tcgcccggct gcatttctag gcggctgtag tcaatagtcc gtccgttgag ccggggcggt
      721 gtgcccgtct taaaccgcaa aacggtgaag cccaattctc gcagtgattg actcagtcct
      781 agaacggcct cctcgccggc gcgaccgcct tccatctttt tttcaccgta atgaagaagg
      841 ccccgcatga acgtccccgt gcacagaatc acgcatgggg cacgatagat tgcatcactc
      901 cgcaccgcta ccccgacgat tcgcaaccca ctcgctgtct tttccaccaa taacgattcg
      961 accgtctctt gacgtaaagc cagattcggt tgggattcgc agatctgctt gaccttctgg
     1021 tgatatgcga ccttgtctgc ctgtgcgcga ggaccatgca ttgccggtcc cttgcggcga
     1081 ttgagtaggc ggtactggat ggcggtcgca tcgatggctt gccccatcgc ccctcccagt
     1141 gcgtcgatct cgcggacaat ctggcccttg gcgacacccc cgatagcggg attgcaactc
     1201 atcttcgcga tcgtgtcgag attactcgtc aggagggcgg tgttagcccc catccgtgca
     1261 gcggccaatg cggcctccac accggcgtgt cccgcaccga cgacgatcac atcaaaatcg
     1321 tagacactcg tcatgtgtta cgaggattgt ctcagagttc ttaggaaggt gtcttgcggg
     1381 ctgtagtgat ggcgatgcaa tcgtaactga cgcggccctg ctgaaagacc attttactcc
     1441 tgcgaataat gcgccatcct ccgttttgaa aaacaccctc cgtctcgatc ttttccacga
     1501 ctccatttgc ggtctctagg tattttgcca gtccggcatg ttgtgtcaga acaatggggc
     1561 gaaaatctgg ccccacgacc agcgtcaccg gttcgatcac aaggttggag agatcggtga
     1621 tcggaggagg ttcgactttg ggacgttgga ggcgaatcat tcgcgctcgg gcatcggggc
     1681 ccaccgtctc tttggcggaa ctgatcaaat cgagcaatcg gtctcgaacc tcggtgagtt
     1741 ccttggccga cggtatttcc cgccatttcg gatcgctttg aatggccttg gcgaggagcg
     1801 atatctcccg aagatattgg ttccgctggt tctccggcac aaggtgcatg atgatgatgg
     1861 acacgcgctc cttgtcgtcg ggggaatagg taatcccgtg ggcgctccac ccaacgacgc
     1921 acaacaagtc accttcaaag gagacccgcg cgtgaggaca tgcccaaccc tgccccaggg
     1981 aggtgtatga agtgcgttcg cgttccagta cgagaccctc gatgtcaagg ccggctggca
     2041 aggagggaat ggcctcgagg attctcgcca gaattcgcag ggcttcctct ttgtcgtcgt
     2101 cttcgggaag ttcgatcagt cgtccctctt ggagggcgtc aaggagactg tccatactca
     2161 cttatctcca tatcgccgga aaaaccaaca tttgacgagg tgcgtcaatg tgccataagc
     2221 aagaagaaaa ctgccaatcc ataaccaata tgttgccgga aggggcacca tccctaagac
     2281 atcggccagt ggtgaatacg gtagccaaat accaacggcc atcacggcca gagttgtcaa
     2341 gatcatcgga gcggaagcgc agctcccgat aaacggaatc cgttgcgtgc ggataatatg
     2401 gacgattaac gtttgggtca gcagtgactc aacaaaccaa cccgtttgga aaaggctcat
     2461 ccagtattct ctatcagttc cgccttgtag atacgcggaa cacccaaaaa tgaaccacat
     2521 tagggcaaac gtaagataat caaaaacgga actgatcggc ccaatgaaca ccatgaaacg
     2581 gcggatgttg tcaatatccc attttagggg ccgagaaact tgctccgcat caacgttgtc
     2641 cgaaggaatg cctgtctgcg acacatcgta cagaaggtta ttcacaagaa tgtggatggg
     2701 cttcataggg aggaagggca gcaagtaact ggccccgaga acgctaaaca tgttgccaaa
     2761 attggagctt gcccccatgc gaatatactt gacgatattt gcaaaaatcc gcctgccttc
     2821 catgaccccc tcttccaaaa ccaggaggtt tttctcgaga aggacaatat cggcggcctc
     2881 tttcgccaca tcaaccgcgg agtccaccga aatccccaca tcggcggcct tcatagcggc
     2941 ggcatcatta atcccatcac ctaagaaacc cacgacttgc ccagcttcgc gcaacttgcg
     3001 gacaatctgc tccttctggc ttgggctgag tttcacgaag acatcgacct cacggacaac
     3061 cctgtcgaat tcctcctcgg tcaaatcggc gagctcgttg cctgaaacaa tccgccggac
     3121 ctcgaggccc acgtcgtgac acactttttc ggtaacgagg gcattgtcac ccgttaaaac
     3181 cttaaccgtg acacccactt cttttagcag tcggatggcc tccagcgccg agtccttcgg
     3241 tggatcatag aaggcaatat agcctagcaa gatcaaatcg gattcatcgg ctacagaaaa
     3301 agtcgtcttg tcccgcggga actcgcggta ggcaactccc aacacacgaa agccttcccg
     3361 attgagtcgt tcgacctcct caaacaaatc gttttggata acgggaatca gcggatatat
     3421 ttcgtcgcca atctggtagt accggcagca ctcgtatatt tcttccacgg cgcccttgca
     3481 gatgaggacg tggtcccctt cgtaatcaac gacgacagac atacgacgcc gctgaaaatc
     3541 aaagggaagc tcatcgacaa ggcgacactg gctgctttcc acgtcgagat ttgcccgatc
     3601 cagtaccgca tgatcgatca gacttcgcaa accggtttga aaatagctat tcaaataagc
     3661 ataggtgagc acgtcgtcac tagggcgccc ggtgatatcc acatgacgtt ccaggacaac
     3721 acggtcctgg gtgagggttc cagtcttgtc ggtgcaaagg acatccatgg ccccaaagtt
     3781 ttggatggcc gggagatgct tgacgatgac ctttcgcttg gccatgctca aggccccttt
     3841 ggccaggttg accgtgacga tcatgggcaa catttcaggg gtgagcccca cggccaccga
     3901 aagcccaaag agcaaagcct ctacccaatt gcctttggtg aatcccacaa tcaggaaaac
     3961 cagggccacc atcactacca tgaaccggat catgagccag gtaaaccccc gaataccttg
     4021 gtcgaagctg gtctcgggtc gcgtttcaat gagccgtgcc gagatcgaac ccagcatcgt
     4081 cctggtcccg gtgtttacca cgacggcgcg ggctgtgcca ctaatgacat tgcttccgag
     4141 gaaacaagca ttcggaagat cccagggact ggtggccgcg ctaccgttga gaaaaaccgt
     4201 cttttcgacg ggcatcgatt ctcctgtaag taccgattgg ctgacaaaaa agtccttggc
     4261 cgagagcaaa cggacgtcgg ccgggaccat ggcgccggcg tggagaagaa cgatatcgcc
     4321 gggaacgatc tctgagatct tgacctcaac ttcacgtccg ccacgtagga caagacttcg
     4381 gggctggaca cgctttccta gggcctccac agcgcgattc gatcggcggt cgagcaaata
     4441 agaaagccca atgctgagga gaaccatcgc cgcgacgaca atggccgatt tccattcacc
     4501 gatgagcccc gaaacacccc caatgacaaa aagctgaatg tttagcggat ttctgcaccg
     4561 gtgtaggatg tccgcccaaa atgaaagcct ttgggcgtgg ggaagttcgt tgtggccgta
     4621 ttcatcgagg cgcgcctcaa cgtctgcctc atcgaggccg gaccattttg tggccaatcg
     4681 ggcgagcgct tcgccggggg tcagggtgca aatctcaatt aaaagcttct cgtgaggagc
     4741 taatgcctga gcgaccctgg tctcctcttt ggaccgggtc cgcagcgctt caatcatgtt
     4801 tcgtggaagc gaccggagca tgaatgtctc cccgagccct caccttcttc ttgccaattg
     4861 ggcggtcaat cgatcttctt ttgtcgaggg ttcctcacct aatacgggac ctgagtgcgg
     4921 gatgctccaa aaagatttcg ccgggccggg tgggaggtcg ccaaatgttc cgtgtcgtgg
     4981 cctaagtcgg tttttgtgcc gggagaggat cgtaaaggcc gcaacgatag ggcgaaagaa
     5041 agacgcaatt gaagttgcgc gcacctgaga atcgaaccga cccatgttgc gcctccggtg
     5101 actccccgaa cccacccacg gccggaggcg ccctgtcggt tcagcggacc atcaagaaca
     5161 gacagacgca aaggccccca accgggcggg aattccaacg ctgatggtca tccacgtcgt
     5221 tatcggacat cttttaactc ttgccgtagc aacaacccgc gtcgtgacga aaatcctgag
     5281 ccacgcaaaa caccaacacg ttctgagcgc tgcggcaccc ctttctttta agtctaagac
     5341 tgcacaaggc ttctttcaag gatcgagatc gggcgaaatc cggttagcca cagtgtcttt
     5401 caaacaatcc aaaccgtggt aatctgagtg gtgagacgat tcttcaccgg gtgttcctcc
     5461 gcacgcccca ggtcgagaat tcagccgcag agtgccctac ctataccatg gttggggaaa
     5521 atgtgaaatc gctggtgatg gtagtaaacg cctcgttgat tcgttataac atacagacgg
     5581 catagggcac ctaataggag gcgtttttat gaccacagtg cttgttgtgg acgactctcc
     5641 gatcgattcc caattggtcg gttcgctgct taaaaagcga ttcgactgga agatcgaata
     5701 tgctgaatcg gggacagaag cgctcgcgcg gatgaaacaa aatgtgcccg atctcgttat
     5761 cacggatatc agcatgcccg agatggacgg tctggaactg gtccagcata ttcggaaaat
     5821 gtttcccgaa gtccccgtca ttttgaccac agcctatggc acggacatgt tggcaatcga
     5881 agccctggaa cgtggtgcag ccagttacgt tccgaaatcc caattggccg cgcggcttgc
     5941 cgaaacggcc gactccgttc tccggattgc agagcagaat cgagagcggg agcgtctttt
     6001 ccgttgtttg gaatacattg agttcgagta cgttttggac aatgaagcag agctaatcca
     6061 gcccctcgtg tcggtggttc aacagaccct ggctgggatg gaatttgccg attttgccgg
     6121 tcgccttcaa ttggggattg ccctcaagga agcgctgctg aacgcgttgt ttcacggcaa
     6181 cctggaaatc agcaagagtg agattgaggc cgtctcggat cacctgctgg aagacagcga
     6241 actgagtttg gtagcacagc ggcgccagga tcctcggtat cgtgaccgca agatctatgt
     6301 cctggttcgc atcacaaaag aggaggcacg gtttgtcgtc cgggacgatg ggcccggctt
     6361 cgatgtgcac agcgttgtcc gcaaaaaaga tgaatccccc gagctggagg cacaacgcgg
     6421 cctgtcgtta attcaaacgt tcatggatga agtcaatttc aatgaaaagg ggaacgaaat
     6481 tactctgatc aaacggcgcg agaagctgtc caaggcctaa tcatttccga gtcgtgtttc
     6541 cacattacag gtcaaatgtg acggggccag gggatggcct aatgttcccg cacttgcggg
     6601 tgctacttgt cgatcatcac gatagctttg tgtacaactt ggggcgatat tttgagttac
     6661 tcggctgccg aattgcgatt gtgcgaccca cggaagtacc cgaatcatcg aacattttgc
     6721 gactctttga ccttgtcgtc ctctcgccgg gtccctgtgg tcctcagaac gtggtgacct
     6781 ccgtggagct tgttcacaat ctcgcagatc aaatgccgat tctcggtgtt tgcttgggac
     6841 atcaaatcat tgccgaggcc ttcggcggga ggattatccg agccgcagaa cccgtgcatg
     6901 gcagggcgac acccgtttgg cacgacggac aacacgaatt tgcgggaatt ccaaaccctt
     6961 ttccggcggg acgttatcac tcgttgatcg ccgatcgaga ttcgatcagc gaggaagtgg
     7021 aaatctcggc atggacagac gatggcatac ccatggccat acgtcacagc cgctacccgg
     7081 tggtgggttg gcaatttcac ccggagtcgg ttctgactcc gttgggactt gatcttttac
     7141 gaaacctgtt actttcgtgg aatcaatcgc tcagatttgc gcggtgacac ttaaaaaggg
     7201 tgtcccttgg agacgaatgt ctcacctgct cattgaagca aaccgtaatc ggcgaggacc
     7261 ttgcgcagcc ggttctcctg gtcagaagaa agcggtgtca acggcagacg taattctcca
     7321 ctgtctcgcc ctagcagtcg catggctgct ttgataggga tgggatttgt ggacaacccc
     7381 agcatgtcac gagccaaggg gaacagcttt ttgtgccaac ggatggcttc tgggatgttg
     7441 ccttcctcaa aggcccgaat catcgcaatc atatcctgcg gcacgatatt cccgatcacc
     7501 gaaataacgc cgcgtccacc cacggagagg atcggcaggg tcaggctgtc atcgccactg
     7561 aggaccgtca agtccgtctg ggtcgtcaat tgtgagacct gatccaaaga gccgcttgcc
     7621 tctttgacca tggcgatatt gggaaactcg gccaagcgga agaaggtctc cggttcgatg
     7681 tttcgtccag ttcgaccggg aatgttgtaa atacaaatgg gaatgtcaac cgcctgggca
     7741 agcgatttaa agtgctgata caggccctct tgcggaggtc ggttgtagta gggagacacc
     7801 accagaacgg cgtcggcccc cacctgagcg gcgaagcgcg tgagttcgag ggcttcttcg
     7861 gtactattgg agcctgtccc cggcatgacc ttgacccggc catttgccgc cttgatcaca
     7921 gccgagataa cctcgcgatg ctcaggatgt gacagcgtgg gtgactcacc agtcgtgccc
     7981 accgggcaaa gacacgtcac accggcctga atctgaaact cgacctgctc ctcaagtcta
     8041 gcgtagtcaa cttgtccatc gcgaaacggc gtaatcaagg caacggacag cccagcgaat
     8101 tgttcagctc gtgtggtcat agatttctcg tgactgttct ggaaagggaa tgcgatggtt
     8161 acgtcgtaac aagcctacga aacggtcaat ggaagtgttc gggatgggat gctgcccaaa
     8221 ccattaggtc cttcatttcg cgaacggctt gaaccattcc gacgagcacc gcccttgcca
     8281 cgatgctgtg cccaatgttt aactcttcca ttcctggaat agccgccact ggttgaacat
     8341 tgcggtaggt caaaccatgg cccgcatgca acttcagccc ccgctgaagg acctcatggg
     8401 aagccttccg caattgctgc aactcctgtt gctggacctt tcccgaagaa ttggcgtact
     8461 gccctgtgtg cagctcgacg gccacagccc cggtcttagt ggccgcgtcg atttgggcca
     8521 agtccggatc aataaacagg ctcacactga tccccgcatc ttgtagtctc ttcacgaagt
     8581 ccgtgacccg ggccaattga ccagcaacgt caagcccacc ctccgtcgtg atctcctcgc
     8641 gccgctccgg aaccaaagtt gcctgatctg gtttgacagc caaggcgatt gccacgattt
     8701 cttccgaaca ggccatttcc aggttcaact tgaccgccgt tgtttcccgc agaagtcgca
     8761 ggtcacggtc ctgaatgtgt cggcggtctt cccgcagatg aatggtgata ccatcggccc
     8821 cgccgagctc tgccagggcc gccgcccata cggggtctgg ctcgtaggtc cgtctggcct
     8881 gtctcaacgt ggcaatatga tcgacattaa cacccaggtg gatcattacc aacagcacca
     8941 ataatactta gcaaaagcga acttcataat cgcaggcaaa ggaaagactt ccttcccgca
     9001 agatccaatc agcgcgatta tatcggcaac aggttcggtc gcaaagaaac ccttcaggac
     9061 aaaacaaact gtcgcaagcc gtaccctcgt tgaaagtcac attggtttgc ggttgtgaga
     9121 ccgtggtagc ctcgcgatga agatcggatt gtgaccaagg ccaaggaagc aaattcaagg
     9181 actcgactca gaaagaagat gccgtccctg ctttcacact ccgccgttgt ttgttccccg
     9241 gtatagtttt cgattgagcg ccggaaagaa ggcagtccaa gtgttatcga agggcggatc
     9301 accctggatt ctctccacgc aggccgccat ccgagaatca agatagtcga gacaatgaag
     9361 ggccattgcc tccaaggtca tgggcagtcg aacacttccg aactcgtatt ccccatgatg
     9421 acttacaagc atatgcttca gccggagcaa aagttcctct ggaaaggtct ctcccaagag
     9481 cttttccacc ttgtccacgg cctcggcgag cagttccaga ccaatggtaa tatggcccaa
     9541 aagttgtccg gaatctgtat aggtcagttc cgagcctccc gcgaattcgt ttatcttccc
     9601 gatgtcgtgg agaaataccc cggccaacag caagtccttg tcgagcaacg ggtagagagg
     9661 tgcgatactc gcagccattt ccagcatatt ggcgatatgg atcgctagac caccgcggta
     9721 agcatggtga aagcttacgg cggccgggct ttcagccagc ttggccatga actcctcgtc
     9781 gagcaaaaac gaatcgatca gggcgcgcaa atgaggattg ttaattcctc gcagccgttc
     9841 tgccaattga ttcattgctc gatcgatttc caacgtgtta accaaggaga agtcagacgg
     9901 atcgacttcc gccgcgggca cacgttgcag gcgcgatacg atcaactgga tcgcaccttg
     9961 aaagatttgg gtggtcccct caacccggac gtagtccccc gcatcgaacc ggttcaaaat
    10021 ggtttcatcg acattccaca tgcgggcatc gagggacccc gttcggtccg ccagtcgcat
    10081 ctgtaagtag aggattccgt tccggttcgg acgcaattga cggcttgtga cttgaaacac
    10141 ctcgttgacc gtttcctccg gccgcaactg attaacatac ttccttgtca tggatgacct
    10201 ttcactaaag tgatgatggc ctattctcgg agcactagtg acctatctca ctccagagtg
    10261 ataagccgca aatcaaaaat tccgcctgcg gtagccccag gatgagcctg gaagcgaact
    10321 gtcacttgct tgccgttctg gaggacattg tcaggaagag gataagtctc cctgtaaaag
    10381 tggcccgggc gattgttggc caatcgctgc gttgcaatag tttgcccatt aaccaggata
    10441 tcaaagacgc gattaccaga gtcacttccc caatattcga cgagtaaggc cagtcgtggc
    10501 ttatccgcga gctgcaaatc gtaagcaaac cacccaccgt tggttgcatg acgccaggcc
    10561 cgtccttgaa acgtcccgtg gctggtgttt tcgccctgga gattatgctg cttctcgctc
    10621 tcaggattgc caataatgac atggtcgata gtcttttttt ccaattcacg ccacttgacc
    10681 agttcctctt gataattggc aatcatttcc ttccaagcct ggtgtgatac ccgatcccaa
    10741 tagacaacgt accgtcgtcc ttcatggatg gcaaaaaacg gctccagcct aacggggacc
    10801 gcctgatctt ccgcgaggga gcgaaacagc ccatccgggc tttgccagac acacggcttg
    10861 ccttcgaccg gtttaaaccg gccgagatcg atcgtttcgc tgacgatcac ggggatttcg
    10921 ggattatagc tacccattgg ggggagtttt cttacctgtt gctgggacat tttcggcaat
    10981 tccgccgcca gcacgactgg cccaaaaaga aaggccaaac gatgaggatt atcacgaaag
    11041 gcctccgttc gcaacgtcat gggtaggtca acaacgatac gatcgccagc cttccaagtg
    11101 cggcgaaggg ggacgaagcc ctcttgcgta gggctttcgg tgacgacctc gccatttacg
    11161 ctgatctgaa atccgtccgt cgtccaaaaa ggtcggcgaa tgtaaatagt cacatctgac
    11221 gatggcgggt tggtgataac caaggtggac cgcggttcat agggaaaatt ggtctcttgc
    11281 cgcagtttca gccccgtttg tgaccactgt agttcggagg ggatgaacag gtttacaaat
    11341 agagcgccct tgccgtcgtg aaagtagatg ctatccccgt actttgcgtg attttcgaca
    11401 cctgttcccg tgcaacacca gaaagaatcg agcggagtcg agtaaacctt atgggagccg
    11461 gaacgaaggg gcacgtaata acacatcatt cccgtcgccg gatcttggga ggcgagaatg
    11521 tgattgtata atgcccgttc gtaatagtcg gcatattggg caaggggctt ccaacagaaa
    11581 agatgtcgcg tcagcttcag catgttatag gtgttgcatg tctcggttgt gttaggaccg
    11641 agtgcttctg aaaggcgttc ttttggcgaa aagtgctcgt tgtcgctgtg gccgccgatg
    11701 acataggacc gttcgttgac gacaaacttc caaaaattga cggcggcatc gtgataccat
    11761 tgttttccgg tcacctcgaa cagtcgcgcg gcgccgatga acttgggaat ttgagtgttg
    11821 gcatgaaggc cggtcaaaac atccgtgccc ctggagagag gctccatcac ggctaggtgg
    11881 ttgaaccgct cggcaatctg cagatacttc gcttcgccgg tcaaggcata gaggttggcg
    11941 agggcctcgt tcatgccccc gtgttccacg ccgagcatcc gttgcatttg ctggtctgag
    12001 agacgttgat tgcgggcgat gacccaatca cctaacttct tggccacttc cagggcttgg
    12061 tcgttttcgc agtaaacata ggcatcgagg agaccagcca ggatcttgtg aagtgtgtaa
    12121 tacggtgccc agacccgccc ggtggtttct acacggtcaa aaaattcctc aggaaaggca
    12181 ctgaggtaac ccccgccgag ccgctcttgg catttcgcta attccgccac catgtaggcg
    12241 actcgttccc ggagggattg atcgcccgtg cttgcaaaca tcaaagaaca tgccgacaga
    12301 tagtgcccca caaaatgtcc gcggagctcg cacttaggat cctcccagcc gccgaggggt
    12361 tggacattac tgggtaatcc cgcgttggag cgaaagacat ggagcaaacg atcgggatcg
    12421 aggctcagca gatatcgctt gtccatttcc atcgcatgtc gaaaaggacc tgccgtaaca
    12481 cgtacgtcgc gaaggtcaaa cgcatacacg ctgggggtaa cggaaagctg gatccgcagg
    12541 gcaggtggtt gatgcgtgag cggttccgat gccgtcgtgc cgcgcagcgg cgcactcgtc
    12601 cacaaaaacc aggttgtcag cataacggtg gcggttgcga ctctcataca gttgcccttt
    12661 ctaaaaccag cctcaaccga gcgtaggtgc attacattga cgatccatta ctatacccta
    12721 tcggacccgc agcggcccgg tcaatgatcg ccaccgccaa gaaacagaga aaccccaccg
    12781 gtcaagggtg gggtttcacg aacatcaatc aggcgggaat gatgtctgct gctcagtggc
    12841 ctttaatggc tcggaagagt cctgagaacg gtcccttcga tgcgccatcg tcgaaatcaa
    12901 gataccacca gccgtcgtgc cactccaagg tcaccttccg ccatcccaac ggaacttggg
    12961 gatagggata gaaggggcca atatagggcc aagctgttgc cgagtactgc ttgggataag
    13021 tgacggctgc gtagttagga taggcagcat agctgggcca agcgtagtta ggcatattcg
    13081 gttgatcgaa ccgagccggg gcagcggctc caggagcgcc aaccgacatt gggagaggac
    13141 ggcccaaagg ttgggctgca ggtgccggtg cggcttcgcg ggagacctct tcctgtgtca
    13201 tagcgatggg caggggccgg atcggacgcg gagcggcttc tggagctacc gtcgcggatg
    13261 tttgcgttgc catgggcata tccgcctgcg gcaactcggg gatagcgagt gtcgggccct
    13321 ccatagcgga tatggccgct gtaggacgaa ccggctcagg ggcaaaaacc gacggtacag
    13381 gatcggctag ctgaacaggt cgttgggcaa cctcacccgc cgccggttgt gcggtcgccg
    13441 agggagcact ggaggatgct attgtacgtt ccggggcaat ggctcctgcg gcagtttgca
    13501 gattcggcga atcagtggca ccggcgggaa ctaccgcgag ctcgttgacg acttgtttga
    13561 ccccagggac ccgggagacc accttttcgg cggccagctt ctgttgctca tcggtcacac
    13621 taccccgaag ccacgcaatc ccatcctggt accggactcc aatcttgtag ccgcgcaatt
    13681 ggccactgtt tttcaatgcg ctggcgatct cttgtgccac ttcctggttg cctgcccagg
    13741 cccatgacgg gcaaatgaca acggcaaccg ccacaatcca actcaatgaa aaccctcgca
    13801 tgcctgtcct cctgttcgga atccacgtcc cgacggtcgg cgccttgttc tggaaggcag
    13861 acttcctttc ccgcctggat cagttacctg agaaacctaa cctcatcttc accggacgtc
    13921 atagacaaaa cgttgcttac aaccgcaaga tggtcacagc aattagtgaa aagccgcaag
    13981 ccatcctgcc tatatacgat tcgacaatct taaataccga acggtatgtt ttttcggtgt
    14041 ttggcaaagt tgtcgaactc ttcccacatt ccgtatcttc caattttccg gcttcggtca
    14101 tttttgtaaa atgctccaag aaacccggcc tcaatcgtcg tatgattagc atcggtaccg
    14161 aaagcccgct tgctccagtc gcatttccaa tgagaccagg agattagaat caactgccgc
    14221 tctttaccgt acttcccgcc tggcagtttt aacagcgcat catttgggcg acgcgagggg
    14281 ggattaaagc cacaagagga cacttcatcg cacaagagga cactttagcc cgatgaattc
    14341 gccttttacg gaagggaagg cccaagagga ccgggcaact gggttaagaa acttggtcag
    14401 acggacttcc tatcggtagg ctgggtaaag tcggccatac tgagaaggat catcacggag
    14461 acggtgacga ggcaggaacc ccacacatga tgggattggc cacacgctgc cagagctatt
    14521 ccagccaata acgctaggat gccatggaaa ccggtactcg cccagtcccg cgtaccggga
    14581 aagcgagttg cgatggccat cgcgcagccc aaaaactgga cacttacaag taatatagcg
    14641 gccacaaggt ggatcacacc gtccctcatc gctttaagct gccgaacacc cttgacttgc
    14701 cacggcttgg acgaacgaga gaaggatagc ttaaaccttt tcgccgataa agcctgattt
    14761 tcgcgatttg ctggcaaagg cttattcact ttgaatctga actttcccaa attgcccagg
    14821 ctgacttttc gcctcacgtg atgtgttagg tacttcatgc tatggttacg ggggaccatg
    14881 aggccgcttt cccattccgc gggtgcgcgc tatttttgat ccgttagcgg accacatcta
    14941 atactcgaaa gaaccgcgag gaaagaggtg tggggaaaat aggcgaggtg cgccatgttg
    15001 gaactggttg atctcaacca acggatttct ggcgaggagt acgaaaagat attcccagac
    15061 ctggaagcgc agctcgcggc gtgtcagcgg atggcccggc agtcggggat acccatcgcg
    15121 attgtctttg agggctggga cgcggctgga aaaggaatga tgattaaccg gctggcccag
    15181 gtcttggacc cccgcggatt tcgcgtttat ttgacggggg atccgagcgc cgaggaacaa
    15241 cgccgtccgt ggatgtggcg gttctggaag gagctaccgg gggcggggga aatcgtcatg
    15301 ttcgaccact cttggtaccg ccgtatcctc gacgacgtgg cccaggggcg actttcgagg
    15361 aaggaatacc agcaggcggt tcatgatatt ctcgaatttg aaaaacaact ggccgacagt
    15421 gggttgctct tgataaagtt ttggcttcac atcagcgcca aggaacaacg gcggcgtcta
    15481 aagcggctgg agcagtcgcc tgctacacgg tggaaagtga ccaaatcaca gtgggagcag
    15541 aaccgcaaat acaagaagtg gcaaaaattg gtcgagacag ccctccagcg cacgagctcc
    15601 tctcaaaacc cgtggacggt ggtcgaggcc aatcagggga agtttgccag agtcaaagtc
    15661 tttcgcacta tcatatctgc aatcaaagag acatgtgccc ggcgagagcg agagccggca
    15721 ccttctttgg cacccatgcc ggaaccagac gaaagccagt tttaccggcc cacgattttg
    15781 gatcgtgtcg atttgactaa gtcgattggc cgagaggagt acgaacggga actcgaggtt
    15841 cttcataaaa aactctatga gctggaacac cgttgctact tggcgaagat accggttgtg
    15901 atcgtttatg agggttggga cgctgcggga aagggtggca caatcaagcg actgacccgg
    15961 gcgctggacc cgagggggta tgaagttgtc cctgttggac ctcccacgac cgaagaaaaa
    16021 gcccatcatt atctatggcg gttttggcga cgcattccca agacgggaca tatcacgatc
    16081 tttgaccgct cctggtacgg acgggtgttg gtggaacgcg ttgagggcta ctgctcgcag
    16141 gaggaatggg aacgagctta ccgggaaatt aatgaatttg aacgccagtt ggccgacttt
    16201 ggcacggtaa tcatcaagtt ctggcttcat atcgattctg atgaacaact tcggcggttt
    16261 gaaaggcgac aatccgatcc ccagaaacag tggaagatta ccgaagggga ttggcgcaat
    16321 cgggctaaac ggaaacatta tgagctggcc gtgatcgaca tgcttcaacg aacgagcacc
    16381 ctgtacgcac cgtggacgat tctcgaagcg aactgcaaac tcttcgccag gatcaaatcg
    16441 cttcgtgtcg tggttgaacg tctggaagct gccacacgtt gattcaaggg aggcaatcat
    16501 tggggcgggg cccgcggcgc cacggccttc gccggtggtt ttttggggag caggggatat
    16561 tcacggttca aggcaaatag acgatactgt tgtgtcggtc gcttaatggg gcaacccacc
    16621 gtttacccgc atgcgtctga agatcgcaca gaacaaaatt agtcagaatg cattagataa
    16681 tctccctgca gtttaccccc caacactgaa gaaaggcgtt agcaccaaga aaggctgtta
    16741 ccgtgctcga aattgtcgct agtctgttgc ttgtccttgg agtggccgaa tcaaaggtca
    16801 acgaaccaaa cccatgtgat gtcacaaaga aaaacccaag ggtggttcaa gtctttccca
    16861 ttggcttggc ctggtccagc catcctgtca gcttttgttt gtacacgtct ccgccctatc
    16921 aatatgtggg atattacgat gcggaacgcc gcctggtcat cgctgcacgt gagttatcca
    16981 gcaatcgctg ggagattcaa cggttgccaa cgtcggtcgg ctgggatagt cacaacagta
    17041 ttgtcattgc ggtggatcga gatggctatg tccacgtggc aggcaatatg catgttactc
    17101 ctttaatcta tttccgcacg gagcaaccgc atgccattag tacattccag cgatttccga
    17161 tggttgggca ggatgagcaa agagttacat atccgcggtt tcttttcaca gctaatggtg
    17221 atttgctgtt cttttaccgt gacggggcca gcggcaaggg acgacagttt atcaatcgtt
    17281 acgatgttgc cactaagtcc tggagccgtt tgttgcagac acctttgctt gatgggggtg
    17341 aagaaatgag cgcctatccc tcgggacccg atttggggcc tgatggcaga tggcacatgt
    17401 gctgggtttg gcgagatacc ccggattgcg caaccaacca cgatattagc tacgcgagaa
    17461 gttccgatct ccttcactgg gagaccattg acggtaaacc tattgagctt cctatcacac
    17521 cgcggacgcc tggcgtcgtt gtcgatcctg ttcctgtcaa gaaggggctt atcaacgtgg
    17581 ctcattatgt cggtttcgat acccagcatc gccctatcgt cacgtatcac aagtatgacg
    17641 agcaaggtta cagccaaatc tataattccc gatgggagaa tggccggtgg gtcattcgcc
    17701 aagcgactcg atgggagtat cgttgggagt tttcgggagg gggaaccatt caggtcgaga
    17761 tatcgtccag ccgcattcac ttactgcccg atggccgcct tgcccagagg ttttcgcacg
    17821 cccgttatgg ttccggtatc tggatcttcg acccagccag tctgtcggtc gtggcaacct
    17881 gcccggcccc tcgggaaatt ccctcagaat tggccgaatt ggaatcggat tttccaggga
    17941 tggaggtgcg atttgctcgc gatgccggga agccgcctgc aggcgaggaa tacgttctcc
    18001 gctgggaaac gttggccgcc aaccgcgatc gacctcgggc cggcccactt ccaccaccca
    18061 ccacattgcg gctctatcgc ttgcaacgag acccgtgatg aaatgttgat gcctaattca
    18121 aacccacagg tctgaggtcg ccgatcgctc ccagcttgtc gctacaagag gaaggctgcg
    18181 atgaaacgtg aacacatctc ccgtgtcgtg ggcgcattat gtcccggtgc gcttaaccgg
    18241 ggcgtgagac gggatcgacc ggaaattctc cctcgacagg gggatgactg cggactcgtg
    18301 ttctattccc gacatgtaac gaacgatgta ggcaggatgc cgatttgttc gagcacgctc
    18361 caggagctcg gctactagcc caagtcccag ggtggtaatg gcgcccgtcg tgatggccca
    18421 agtggccagc acaatggcga gaatcgtcaa ggaacggctt tctaacgtca gtagtgaaag
    18481 aaggagggcc gttgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccgg
    18601 aatctccacg ccggttatct tgcggatgag ccagttggcc attaacgaag gcacacgacg
    18661 ggtaagccag tggtcgtggc ggtgcttccg ccagccgtgg acaatgtcat agcctttttg
    18721 cagcgtttca atgagatggg gaatatcgtg aggatcattt tgcaggtcgc catccatggt
    18781 cacgacaatc tcaccatgcg tgtggtcaag gccggcttgg atggctgcgg attgtccaaa
    18841 tttcctcgtg aggtgaatca cccgggtgtt gggaaatgta tgactgattt gatcgagcag
    18901 gaggctagtc ccgtctgtgg agccgtcgtc aacgaagcag atttcccagg gcatggacag
    18961 gctttcgagg acgggaacga gctctttctg caacgccgcc acgttcccga cctcgttgtg
    19021 aacgggaatc actacggaaa ttgtcgtcgt cgctggagga aaattgctgc tttctgggct
    19081 tccatccatg atcaaaaccc atcttcccgg tattttgccc ggatgccaaa agacgtccat
    19141 gagaagcaga agtatattga caccgtagag tggatttcaa gttcaaatta gccgtgacac
    19201 caacctggaa aaccgcgcaa cataggcaaa aaacaagcgg ctgtcggtgg taagaccggc
    19261 aaccgctaaa accgtttcgt cgcgcctgtc tcagtggaga actgttaccg agttcgtgaa
    19321 ggtgaaggac ttaacctccc taattaagtt ctttcgaaca atcagatgtc ggtccagaat
    19381 ccggtagcaa tcagccagtc cacgatgccc tcaatgtgct ttgtgttggg cgggttgtta
    19441 cctcggacct tgaccttctc catctgagtc agatagacgt agtacggtag gtccggattc
    19501 tggagcaggg cctcgacgct ggttgcatac aggtggtccg caccagcgga atcatacacc
    19561 acacccgtgt catagccacc accgccatag acttcaatgc gttcgaagaa tttcgccctg
    19621 ttgtagaatc cggaaccctt caaacgggca aatgtcggat ccacggtcag gctgtcgtcg
    19681 cctggcgatc cgta
//
LOCUS       DAOSUT010000076        19085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1840_length_19085_cov_9.708671, whole genome shotgun sequence.
ACCESSION   DAOSUT010000076 DAOSUT010000000
VERSION     DAOSUT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_19085_cov_9.708671"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="PLD05_08645"
     CDS             <1..2355
                     /locus_tag="PLD05_08645"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOP77547.1"
                     /translation="EVAWAVGGAGGAYFFATDGVLRVEVYKRDLHRYNRVTELRAILV
                     GPDRRVLAEARIPDDGLPSGKGPGPFQMVRLETKVDRPGVYGLNVTISQDRYGDEIAW
                     GFRTNCPRYLIETARGHRDEAHREPIVLRHPSKSGDVVFLPRSGEFSLDVSGLPSGVT
                     ALEVFDAAGKLLAKIPVTATRQATHRFPAKVSRDAVPWRIHFPKQQGVLHIDGVTQWE
                     PGDRYRDLTVWTPDPTSWFDWLPNRWLIFPYRRVVYSEPQREGRITFQVRNSAATART
                     FAVLAEFAGEPWPVEIDGPQSLELKAGETREVTLRYQAGPAGETRECFIRVSPQDGSG
                     ITTYGRLTVISGKAPAESPLTLPLILRPYEHENEQLGYLPDYPVASQVYFDMENRPYV
                     SSGGVLHVWDGQRWENRNLSAIVRWADGKRAVRSASALTPKIAFDRLNRVYLVAQVDG
                     RPCLLVSRDGARTFTAYELPRSEGDGRTFDVEVFTGHNTLDGPPPILRYTFLFADPKV
                     FWRRLYRLELILAEYHGEDVVFSQPVTISEKVLGHSAHSGSPSCVVSREGRVHVIWSE
                     ATDPAEKVPGAPTYVVTYDREKAALSQPAFVGYGPPANDVHNTPSITMDSRGYLHTLG
                     GTHGAPFPYARSLVPNDSSKGWTEPQSLGEGLRQTYIGLVCGPNDTLHAVFRLWKSQE
                     PPHPLSTFATLSHQQKPPEKGWDPPQVLVIPPFSEYSVFYHRLTIDRLGRLFVSYDCW
                     STYWFYRNDHPGTRRALLVSSDQGRTWKLATNADLTQLVPLPSR"
     gene            2698..3540
                     /locus_tag="PLD05_08650"
     CDS             2698..3540
                     /locus_tag="PLD05_08650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOP77548.1"
                     /translation="MAELGEVYTAGRVQIYIGDCRQILRRLPAESVQCCITSPPYWGL
                     RDYEHPDQIGAEETPEEYVRNLVSVFREVGRVLRADGTLWLNVGDAYARNGGTGLCGP
                     NAKVGNTRKLIQKRNCKVPPCWGLKDRDLMGLPWRVAFGLQADGWILRSHIVWIKKAP
                     MPESVRNRPSRATEDIFLFAKNPKYYYNNQAVRERTGANLKNYWVLGPEPCDLEHPAA
                     FPSELVRRCILLGSRPGDLVLDPFSGSGTTGVVAAELQRQAVLIELNPGYARQSRNRI
                     TASA"
     gene            4226..6289
                     /gene="tkt"
                     /locus_tag="PLD05_08655"
     CDS             4226..6289
                     /gene="tkt"
                     /locus_tag="PLD05_08655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOP77549.1"
                     /translation="MEQSDRSIEQLAINTIRTLSMDAVEAAKSGHPGTPMALAPVGYV
                     IFNDFLRFDPSRPLWINRDRFVLSCGHASMLIYSLLHLAQVEQVDEHGRPTGELAVPL
                     EHIRQFRQLLSRCPGHPEYRHTSGVEVTTGPLGQGVATSVGMAIAGKWLAARYNRPGY
                     DLFDYGIYVLCSDGDLMEGVSSEAASLAGHLKLGNLCWIYDDNKITIEGHTNLAFSED
                     VAGRFRAYGWRVLQVDDVNDLPALRAAIKEFNPAGAQPTLIIVKSIIAYGAPNKQNTH
                     EAHGAPLGEEEIRLAKQFYGWPVEEKFRVPAEVVEHFRQGIGTRGRRLREQWEKKFAE
                     YEKQYPQEADEIRRILNGELPDGWDSEIPTFATDAKGLATRASSGKVLNAVAKRIPWM
                     IGGSADLAPSNNTLLTFPDAGHFSAENYAGRNLHFGIREHAMGAVANGLALCGLRPYA
                     GTFFVFSDYMRPAIRLAALMELPILYIFTHDSIGVGEDGPTHQPVEHLAALRAIPGLI
                     VVRPADANEVAEAYRLALQVRRNPVALVLTRQNLSTIDRTQYAAASGLRCGAYILADA
                     EGGKPDVILIGTGSEVSVCLAARELLSAQGIAARVVSMPSWELFENQPEEYRHSVLPP
                     EVTTRVAVEAGVGLGWERYVGPHGAFVGMRSFGASAPAGVLMKYFGITAENVANVAKA
                     LIKQD"
     gene            6508..6822
                     /locus_tag="PLD05_08660"
     CDS             6508..6822
                     /locus_tag="PLD05_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77550.1"
                     /translation="MTSSHDPDKPSSSRGWPQVLGRLFLYARSVGSNLHTHAEAIGPL
                     ANGMGLALVIGGAIWLLASRHPLALAMVFLGTALYLATRFPGGPEKPSPPAGKQPKPR
                     QG"
     gene            complement(7008..7562)
                     /locus_tag="PLD05_08665"
     CDS             complement(7008..7562)
                     /locus_tag="PLD05_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOP77551.1"
                     /translation="MNSLQGYLLVASPYLADPNFVGSVVLIIQHTENGAFGVILNRIH
                     PKKIKDLWKEVSESPCRYDRHLNIGGPVSGPLMALHDDPSLAELEVIPGVYFSVQRDN
                     IEQLMAAQGHEARVFLGHSGWAGGQLEGELAQGSWLLAPATKEIVFSDDERHLWQKVA
                     FAVGRAILADAIRVKHRPEFPWLN"
     gene            complement(7972..8403)
                     /locus_tag="PLD05_08670"
     CDS             complement(7972..8403)
                     /locus_tag="PLD05_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77552.1"
                     /translation="MTSLMLSASLLIAMAGAPGDVMPVTQEVVVAEEVPVSGHIAATG
                     HRPTATGHGWLVDWFGPMPQTCYQPRFGCYPGNPRTIHRYPAFHGYYYRAPYNYRHLF
                     EYPWHAQPYESEPLRAQSAEVLIDPTASTSTLQPVPEQALQ"
     gene            8758..10182
                     /locus_tag="PLD05_08675"
     CDS             8758..10182
                     /locus_tag="PLD05_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOP77553.1"
                     /translation="MATTSVIYVISDDPRLGEQVEQAVGDLFSVERLASFDALERLGP
                     GYCTVCPAVVAHLTKKTVNGFPAKELLQKLGQLERIAPVITIGDENLTKEVTGQANGA
                     GRVNFPAAFRPEQLRQAVAELSPVEWELAELFHYRPHAVLRGGHRQWVTFTPSVFEMV
                     EELRVAAAHDVSLLLIGETGAGKTFLARTIHEISPRAGKRFLTVACGALPPDLIESEL
                     FGYVKGAFTGADTDKPGKFAAVDDGTLLLDEIDVLAPEHQAKLLRVIETGEYEPVGSN
                     ETAFSRARLIVASNIDLHQLVEQGKFRRDLFYRLNMLSFFIPPLRDRPYDIDYLARKF
                     AVEQSRSLCVRLRGIDPAFMAALHNYHWPGNVRELENIIRRAVLYCRDGILRVSDLPS
                     MMRPAPRENGGEGLPEISVRWTLDKRLEDAERRILEEALRRNGYRRQDTARELGISRI
                     TLYHKMKKYGLMGNHRDEVSAVLE"
     gene            complement(10186..10575)
                     /locus_tag="PLD05_08680"
     CDS             complement(10186..10575)
                     /locus_tag="PLD05_08680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOP77554.1"
                     /translation="MGRPPVKRRRSWVEKFQAAFRGLWYGIVGQSSFAVHFAVAFLVL
                     VVAAILGVTETEWCILILSIGVVMTAELINSALEWFAPAVTQEYNRYIQTALDVASAA
                     VLLASLGAAVIGFIIFGEHLVQILVRP"
     gene            11101..12237
                     /locus_tag="PLD05_08685"
     CDS             11101..12237
                     /locus_tag="PLD05_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77555.1"
                     /translation="MQSLIRRIHESPWKLVLVAAGGGIQGIADLFHEPGASRTVLEAV
                     IPYSAAALEAWLHGKPDHACAKATARSMAMAAFLRARQLQQAAGETDDSPVAGVACTA
                     SLVTERPKKGPHRVHVAIQRADVTRSVSLFLIKGARSRFEEEQVASGLFLYAMATACD
                     LEVDWALPLLSGEVLEHEKAEAPKSWQELILGTCDVVPLGAPADWQRGRPVVLFPGAF
                     NPLHVGHLRMARVAEAKLGRPVEFEISVLNADKPPLDYVEIRRRVEQFTLQDRIWLTR
                     LPTFLEKARRFPQATFVVGIDTLMRIADPRYYGHNPDARDQAIRELAERGCRFLVFGR
                     CWQGRFLGLKDVDIPEDLRRLCTEVPESEFREDICSTQLRAQEE"
     gene            12499..13260
                     /locus_tag="PLD05_08690"
     CDS             12499..13260
                     /locus_tag="PLD05_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOP77556.1"
                     /translation="MVSVEQLTKYYDDLRRGRIVAVDGISFSASPGEIFGLLGPNGAG
                     KTTTLRILSTLLKPTAGRVLINGYDGLTQPDQVRRSIGFMSVNTAVYDRMTAWEMVEY
                     FGMLYGLDRQELRDRMERLFDRLKMQEIRDLLGSKMSTGMKQKVSIARALVHDPPVLI
                     FDEPTAGLDVLVARALLTTIAELRDQGKCVIFSTHIMREAEKLCDRIAILHRGQILAM
                     GTLEELREQSGENDLEELFFQLITEAERKRADRAS"
     gene            13303..15606
                     /locus_tag="PLD05_08695"
     CDS             13303..15606
                     /locus_tag="PLD05_08695"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOP77557.1"
                     /translation="MMTWRNIWIVFAREIRDQLRDRRTIFMVAVLPVLLYPLLGMIFF
                     QLAQVMGEKPSRILVIGAREPLEPPLFDETGFVSSVFVSPQDAVLLKVEFLSDEVLKA
                     RAGSASEDPGRALAEEALRTEHYDAVLYFPPGFAAQLADFREGIRQQIAAGLSSEANS
                     EAKPGSEKSPEEAGEDASQKQQPTPRTVVVSRSAIPSPRVYYSTANDRSQTAYRRLVE
                     LLDRWTDQVMVANLAAARLPPDAARPFVFETADVAVQSGFRGAAVWAKILPILLVMWA
                     LTGAFYPAVDLCAGEKERGTLETLLSSPARRSEIVLGKLGAITVFSMVTAVLNLISMG
                     LTGWLAFGHLPDFAAPPLFAIFWLAVALLPVSLVFSCLALGIAAFARSTKEGQYYLLP
                     LLFCTLPLVGVPMARGSELDLGTALMPITGLVLLLKTMIEGQMAEALRFVFPVGLVTL
                     GGCYFALRWAVYQFNSETVLFRESERFHLVAWLRHIFRQRAVPSGAAALTCGLAILLM
                     RFLLTNILTRIGVSWNLVQQALISQVVVILLPVVVLAALLTREIRATFLLRLPSWRAV
                     IGAVLLAVMLHPAFFTWQQVIVRLYPLDPVLKESLKQLTGQALDLRQLLLVMALLPAI
                     CEELAFRGFILTGFRSSLRPTHALIFAALFFALGHTILQQSMVAFAAGLILGVIAIQT
                     GSLLPCVIYHAIHNALLILQGSLGQSRWVSDPWIKAMMTWTDEGPTYHLSVVVFGILA
                     AGLIFAWMISRPEVLRSYLAEEETGSH"
     gene            15623..16975
                     /locus_tag="PLD05_08700"
     CDS             15623..16975
                     /locus_tag="PLD05_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP77558.1"
                     /translation="MTSAADLCVTADWILPLDGPPIPNGRITIAGHRIAEIDAGGERV
                     RLMRHDPRPQGVTHLHYKDCVILPGLINAHTHLDLSGWSGDFISPPKLWDWIPEVVRF
                     RRSNAYRPGEAIRDGLQAVLAGGAVGVVDVIPPDWFTWGLVPEQGADPDGDQQMPEWE
                     PSRLRVLSMIECLAPTGRMRIPPEELLQHHAMACRQMGWEIGLSPHAPYTVPMSVLKE
                     VVKVAVDHNATLTMHIAETEEEAVFLSTGGGAFRDMLKHLSAYRDGVFSGGNSLQDVL
                     EVLVQAPRVLLIHGNYLGRSQIDFLCQFRDRVAVVYCPRSHEFFGHTPYPLEQFLEAS
                     IPVLLGTDSRASHPDISMLGELQAVRRKHPHIEAETNIRMATSTAANVLGWENDLGNL
                     AVGRLASFTVVHVDGLASKKDPYEAVLSPHAQVCAVWLAGKLAYQQTGHAGEQEGSVA
                     "
     gene            16972..17931
                     /gene="miaA"
                     /locus_tag="PLD05_08705"
     CDS             16972..17931
                     /gene="miaA"
                     /locus_tag="PLD05_08705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOP77559.1"
                     /translation="MNHGRRLDIRDCVWIVGPTAAGKSEVGVALAELIGGEVISLDSM
                     AVYRGMDIGTAKPTVEQRQRVTHHLIDIVDPWEEYSVARYLADAATCLEDIRRRGKIP
                     LFVGGTPLYLKAVLRGLFEGPQADWEFRQHWRALAETNPPGFLHAEVAKVDPASAARL
                     HPHDWRRLIRALEVYHKTGRPLSEWQSQFDKPREEAMGRVVVLDWPRDILYRRIEARV
                     DAMFDRGLVAEVKRLVADPRGFGRTARQALGYREVLDYLAGKMSLEDTINLVKTRTRQ
                     FAKRQLTWFRHLEECRNLPINEPWQPEDLARRIARDLSLTPLS"
     gene            17994..18386
                     /locus_tag="PLD05_08710"
     CDS             17994..18386
                     /locus_tag="PLD05_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOP77560.1"
                     /translation="MMKNERLKKEELEYYHKLLLRLRERLVGNVKYLTDAALKQAQDV
                     AATRAGSTLHPAEAGSDNFEQEFTLSLVENEHAVLEGIEAAIKAIEEGTYGICEVCGC
                     RIPKARLQALPYATMCIKCAEKTEKGQA"
     gene            complement(18600..>19085)
                     /locus_tag="PLD05_08715"
     CDS             complement(18600..>19085)
                     /locus_tag="PLD05_08715"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706149.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOP77561.1"
                     /translation="ERRGRISEYAEKFKCEFYPNQKPWGVPCDIALPSATQNELNGDD
                     AETLVKNGCQCVSEGANMPSTPEAIEVFRRAKILFAPGKAANAGGVAVSGLEMSQNAQ
                     HTSWTREEVDRRLKQIMQNIHETCCRYGAKPDGSVDYVDGANIGGFVRVADAMLAQGV
                     L"
BASE COUNT         3887 a         5065 c         5794 g         4339 t
ORIGIN      
        1 gaggtcgcct gggccgtggg cggcgcggga ggggcgtatt tcttcgccac agatggtgtg
       61 cttcgtgtgg aggtttacaa acgggacctt catcgttaca accgcgtcac ggaactgcgt
      121 gccatcctcg tgggtcctga tcgccgagtg ctggcggagg ctcgcattcc cgatgacggc
      181 ttgccaagcg ggaagggacc tgggcctttt cagatggtgc gattggaaac gaaggtggat
      241 cgccccggcg tgtatggttt aaatgtcaca atttcccagg atcgctacgg agacgagatt
      301 gcctggggct ttcgcaccaa ttgcccacgt tacctgattg aaacggcacg gggtcatcgg
      361 gacgaagccc atcgggagcc gatcgtcctt cgccatccca gtaagtctgg agatgtggtg
      421 tttcttcctc gctctggcga attcagtctg gatgtgagtg ggctaccttc aggggtgacg
      481 gcgctcgagg tatttgatgc cgcgggaaaa ctcctggcca aaatacctgt gacggccacg
      541 aggcaggcca cgcaccgctt tccggcgaag gtttcgcggg acgctgtgcc ttggcggatt
      601 catttcccca agcaacaggg ggttttgcac attgatggtg tgacacaatg ggaaccaggt
      661 gaccgttacc gcgatttgac agtctggacg cccgatccaa caagttggtt cgactggctt
      721 cccaatcgtt ggcttatctt tccttatcgc cgcgtggtgt acagcgaacc gcaaagagaa
      781 ggaagaatca ccttccaagt gcggaacagt gcggcaacgg ctcgaacctt cgctgttctt
      841 gcggagtttg cgggagaacc ctggcccgta gagattgacg gtccccagtc cctggaactg
      901 aaggcgggtg aaacgaggga agttacattg cgctaccagg ccgggcccgc gggagaaact
      961 cgggaatgct tcatccgagt aagtccccag gatggatcgg gcatcacgac ctatggccgc
     1021 cttacggtta tttccggcaa ggcccccgcc gaatctcctt tgacgctgcc cttgatcctt
     1081 cgaccatatg agcacgaaaa cgaacaactc ggttatttgc ccgattatcc cgtggccagc
     1141 caggtttatt tcgatatgga gaaccggcct tatgtcagtt cgggcggcgt tctgcacgta
     1201 tgggatggcc agcggtggga aaaccgcaat ctatctgcca tcgttcgctg ggccgatggg
     1261 aaaagggctg tgcgttctgc gagcgctctg acgccgaaaa ttgctttcga tcggctcaat
     1321 cgggtgtatc tggtggccca agttgatggt cgtccatgtt tgctggtctc ccgcgatggg
     1381 gcaaggacgt tcacggccta tgagttgccc cgtagtgaag gtgacgggcg aacattcgat
     1441 gtggaggttt tcaccggaca caacacattg gatggtcctc cacctatcct tcgctacacg
     1501 ttcttattcg cggacccgaa ggtcttttgg cggcgcctct atcggctgga gttgatcctg
     1561 gccgagtacc atggggaaga cgtggtattt agccaaccag tgacgatttc ggagaaagtc
     1621 cttggtcatt cggcccactc cggaagtccc tcctgcgtgg tatcccgcga aggccgcgtc
     1681 cacgtcattt ggtcggaggc gaccgatcct gcggaaaaag tgcccggtgc tcccacgtat
     1741 gttgtgacgt atgaccgcga gaaggccgct cttagccagc cggcatttgt cgggtacggt
     1801 ccaccagcga acgatgttca taatacgccg agcatcacca tggatagccg ggggtatttg
     1861 cacacactgg gagggacgca cggggcaccg ttcccatatg cgaggtcgct cgttcctaat
     1921 gattcaagca aggggtggac ggaaccacaa agcctcggtg agggtttgcg acaaacgtac
     1981 attggccttg tgtgtgggcc aaatgatacg ctccatgcgg tgtttcgcct ttggaagtct
     2041 caggagccgc ctcatccttt aagtaccttc gcgacccttt cgcatcagca gaagccgccc
     2101 gaaaaaggat gggacccgcc acaagttctt gtcattccgc cattttcgga atatagcgtt
     2161 ttttaccatc gattaacgat tgaccgcctt gggcgattgt ttgtgtcgta tgattgctgg
     2221 tccacgtatt ggttttatcg gaatgatcac ccgggaacac ggcgggcatt gctcgtctct
     2281 tctgatcaag gccgcacctg gaagctggcc accaatgccg atttgaccca acttgtgccg
     2341 ttgccctccc ggtgaactga gagggtaaag ggcagtgccc tctcaacgtg atgagtcaag
     2401 ggtctttggt ttcaggggag gagaaggctt ctaacgtgat cggccacgac ggcgagggat
     2461 cctccatctg cgacagacgc aagagagatg ggccgctatt gcaagacacg gtgtgttttt
     2521 taacgcggta tcgataatcc ttcattcgag ctgccaacgt aaactggcgg tgaggcgccg
     2581 caattgcccg aaaacgtaga ttttcagatg gcttgacgtc ccatccttct tgggtcgaga
     2641 atgtagcaag ggaaactgga ctcttaaact tctgtggtgt gcctgatgga tggctcaatg
     2701 gctgagttgg gagaagtcta tacagcgggg cgtgttcaaa tttacatagg cgactgccgg
     2761 cagattttgc gccgtttgcc ggcggagtcg gtgcaatgtt gcatcacgtc gccaccttat
     2821 tggggactac gggattatga acatcccgat cagattggtg ctgaggagac gccggaggag
     2881 tatgttcgaa acttagtttc ggtgttcaga gaggtggggc gagttcttcg ggcggacggg
     2941 acattatggc tgaatgtggg cgatgcttac gcgcgaaacg gcggcacagg gctgtgtggc
     3001 ccgaatgcca aagtcggtaa cactcggaag ctgattcaaa agcggaattg taaagtgcct
     3061 ccgtgctggg gcctcaagga tcgggacctg atggggcttc cgtggcgggt ggcttttggc
     3121 ttgcaggctg acgggtggat tttgcgatct catatcgttt ggatcaaaaa ggcgcccatg
     3181 cctgaaagcg ttcggaatcg gccttctcgc gcgactgagg acatctttct gtttgcgaag
     3241 aacccgaagt actactacaa caaccaagcg gttcgcgaac gcacgggagc caatcttaag
     3301 aattactggg tcctggggcc agaaccttgc gatttggaac atccggcggc gtttcccagc
     3361 gagctcgtcc gtcgatgtat tcttttgggc tctcggcctg gagacttagt gctcgatcct
     3421 ttttcaggct caggaacgac tggggtggtt gccgcagagt tgcagcggca agccgttctg
     3481 atcgaactta atcccggtta cgcccggcaa tcgcgaaaca ggatcaccgc ctcggcgtag
     3541 ccctgcggtg ggagggacgc ggtcttccgc gtccgccatt tttcttgagc atcgagtaca
     3601 aatgctccag gtgatgatcg aaggcaatcg tcttcgggac attagtgatc tccccctcga
     3661 tctgatcgtt taggctctcc ggttgcaccc ctttttgagt tcttcctgag agtatccctg
     3721 ctgggtcagc tttctctgtc aaaagggtca gaaatcggtg cttttgccgc cgggcccaga
     3781 agggtgtgaa cgatcagaat ccgcgtcaag cttttccgaa cttcacgaga cgacgttcgg
     3841 tgcccagaca tggcggacgt cgccgggtct gccattctac gccagaaaag ggggaacgat
     3901 ttcgcggcga cgggggtcgg taggccttgg agaagttatg gcgtgggatt cgcgcgtcga
     3961 aaagcatagg tttcactagg agaacttggc gcctaggagg taaccgcgaa ggccctttca
     4021 aattacgcat ctcggctgtg ccaccacgtt gacggtcact cgtgggcgac ctaaattaaa
     4081 cgttcacgta tttggaagat gaagccgcca atgaagagaa aactgatcag ttcaccggtg
     4141 ttttgtccaa aacggggaag ttggccattt tgatttctcg ttgtgtcaat cagcaagtcg
     4201 gttagtacac aagggagtta ctgtggtgga acagtctgat cgttcgattg agcaactggc
     4261 gattaacacg attcgcacgc tttccatgga tgccgtggag gcggcgaaaa gcggtcaccc
     4321 ggggacgccg atggccttgg cgccggtggg gtatgtgatt ttcaacgact ttttgcgatt
     4381 tgatccgtcg cgtcccttat ggatcaatcg ggaccgcttt gtcctctcgt gtggtcacgc
     4441 gtcgatgttg atttactcgc tgctgcacct cgcccaggtg gaacaggtcg atgagcatgg
     4501 tcggccaacg ggggaactgg ccgtgccttt ggaacacatc cggcaattcc ggcaactgct
     4561 cagtcgttgt ccgggtcacc cggagtaccg gcatacctcc ggggtggagg tgacaaccgg
     4621 cccactcggc caaggagtgg cgacaagcgt gggcatggcg attgcgggaa agtggctggc
     4681 agctcggtat aaccgtccgg gttacgatct gtttgattac ggcatttacg tcctttgcag
     4741 cgatggtgac ttgatggaag gcgtttccag cgaggccgcc tcgcttgccg ggcatttgaa
     4801 acttggtaat ctttgctgga tttacgacga caacaagatc accattgaag gccacacaaa
     4861 tttggccttc agcgaagacg tggcagggcg gtttcgtgcc tacggctggc gagtgctcca
     4921 ggttgacgat gtcaatgacc taccggcgct acgtgcggca atcaaagagt tcaatcctgc
     4981 cggtgcgcag ccaacgctca ttattgtcaa gagcatcatt gcctacgggg cccccaataa
     5041 gcaaaacacg cacgaggccc atggggcgcc ccttggtgag gaagaaatcc ggctggccaa
     5101 gcagttttac ggttggccgg tggaagaaaa attccgcgtt cccgccgagg tcgtggagca
     5161 tttccgtcaa gggattggca cacggggccg aaggcttcgc gaacaatggg aaaagaaatt
     5221 cgcggaatac gaaaaacagt atccccaaga ggccgacgag attcgtcgca tcctcaacgg
     5281 tgagctaccc gacgggtggg atagcgagat tcccaccttc gccacagatg ctaagggatt
     5341 ggcgacgcgg gcgtcctctg ggaaagtcct aaatgccgtt gcgaaacgca ttccgtggat
     5401 gatcggcggc tcggcggact tggctccgtc caacaacacg ctcctgactt ttccggatgc
     5461 cggacacttt tcggcggaga attatgccgg gcggaacctt cacttcggca tccgtgagca
     5521 tgccatgggg gctgtggcga atggtttggc gttgtgtggc ttgcgtccgt atgccggcac
     5581 gttctttgtg ttcagcgatt acatgcgtcc ggccatccgg ttggcggcgc tgatggagtt
     5641 gccgattctc tatatcttta cccacgattc gattggtgtg ggggaagacg gcccgacaca
     5701 ccagcctgtg gaacacctgg cggccttgcg ggccattccg ggtttgattg tggttcgacc
     5761 ggcggatgcg aacgaggtcg cggaggcgta tcggctggcc ttgcaagttc gacgaaaccc
     5821 ggtggctttg gtgctcacgc gacagaatct ttccaccatc gatcgaactc agtatgcggc
     5881 ggccagtggg cttcgatgtg gggcctatat cctggcggat gccgagggcg ggaagcccga
     5941 cgtgattctg atcggaaccg gaagcgaagt ctctgtatgt ctggccgcgc gcgagttgct
     6001 ctccgcccag ggcatcgccg cccgggtcgt aagtatgcca agctgggagt tgttcgaaaa
     6061 ccaaccggag gagtaccgtc attcggtgtt gccaccagag gttactaccc gggttgcagt
     6121 ggaagccggc gtagggttag gctgggagcg ctacgtaggg ccacatgggg cctttgtagg
     6181 catgcggagt tttggggcct ctgcaccagc cggggtgctc atgaagtact ttggcatcac
     6241 ggccgaaaac gtggctaacg tggccaaggc cttgatcaag caggattgac cagagacaag
     6301 caggcgtgat tgggcgacta agagcctatc ccataatctc cccccacgcg ttcggcggga
     6361 gaagggccgt ggagaggatt gcgcgaatcg ctgccggttt tctcgatcaa ctcggacgtt
     6421 cgcttgccct caccccaacc ctctcccgga ggccgagggg gtctttttgg ggataggctc
     6481 taagcgccga gcgttcggcg atgttcgatg acaagctcac acgaccctga caagccgtcg
     6541 tcatcccgtg gttggcccca agtcttggga cggctctttc tgtatgcccg aagcgtcggc
     6601 tcaaatttgc acacacacgc cgaggctatt ggcccactgg ccaacgggat gggattggcg
     6661 ctagtcattg gtggtgcaat ctggcttttg gcgagccgtc atccgctggc cctggctatg
     6721 gttttcttgg gaaccgcctt atatctggcc acccgttttc ccggtggacc ggagaagccg
     6781 tcccctcctg ccggtaaaca gccaaagccg agacagggat aaaccaggat ggcgtcgcgc
     6841 aaacggccgt gggaatcatt ctgagaagcc gcgccgttgc ggctttgatc tgggccttca
     6901 ctcttttgct agcgtcaccg acatgtttta aggcggagca ctagtgcaag gtctcccaag
     6961 aaaagacgct gcggccagtg ggcgacaagc cgcctgggaa ggctttttca gttcaaccac
     7021 gggaattccg gacggtgctt gacgcggatc gcatccgcca gaatggcccg ccccacagcg
     7081 aaggcgacct tttgccagag atgccgttcg tcgtccgaga aaacgatttc cttggtggcc
     7141 ggggccaaca accaagaccc ctgcgccagt tcgccttcga gttgtcctcc tgcccagccc
     7201 gagtggccca agaacactcg ggcctcatgg ccctgagccg ccatgagttg ctcaatattg
     7261 tcccgttgaa cgctgaaata taccccaggg atgacctcta gttctgccag ggagggatca
     7321 tcgtgcaggg ccatcaaggg gccactgacg gggccaccga tgttcaaatg ccgatcgtac
     7381 cggcaggggc tttcggacac ctctttccat agatccttaa tctttttggg atgaatccgg
     7441 ttcaaaataa cgccaaacgc cccgttttcg gtgtgctgga taatcaacac caccgaaccg
     7501 acgaagttcg gatccgccaa atagggggac gccacgagca gatatccttg cagcgagttc
     7561 atctttgtcg tcctcccgtt aggtgttccc gttaccctgt gcttccaagc caaactacct
     7621 ccactagtaa tatatacgca cgacacgacc aagcatttgg ggcttcctat tcttggatgc
     7681 cagagtgacc gcgtaaagag gggttcctac cggtgggcgc gataatggac cgacccgggg
     7741 ggaccgagaa gcatcgttga aggaaaagcg tcccagcctt caggggggat agtcgcccgc
     7801 gctggccgac ctcgtgacgg cggttttcgc agtcacgccg gaaagccatg aatttcgcgg
     7861 atggctacag aggatatagc gtaactagcc gaacggccgt agcggtggtt tacactggcc
     7921 gaaagcccgt tgtgagtgct aagcggctcg tcgtggccgg gttggtggac tctattgtag
     7981 ggcttgttcc ggcacgggtt ggagcgtgga tgtgcttgca gtcgggtcaa tcaagacctc
     8041 ggcactttgg gcacgcaatg gctcggactc gtagggttgg gcgtgccagg ggtactcgaa
     8101 gaggtgtcga tagttgtacg gcgcccggta gtagtagccg tggaaggcgg gataacggtg
     8161 gatcgtccgc ggattaccgg ggtagcatcc gaatctaggc tggtaacagg tttgcggcat
     8221 gggcccaaac cagtcgacga gccagccatg ccccgtggct gtcggcctgt ggcccgtcgc
     8281 agcgatgtgc cctgagacgg gaacttcttc cgcgaccaca acttcttggg taaccggcat
     8341 aacatcgccg ggtgctcctg ccatggcgat taagagtgat gccgacagca tcagggacgt
     8401 catgatttgg tcctcacaat aaaacttgat gggccaagag atagggttcc tcttttttgc
     8461 tatcggcgcg gttgcccagc aaagctgata aatccagtct tgatcgctaa actctgcacg
     8521 accggaccgc acggacaggc ggaagagctg attttcctcg gaacactggc ggacccgact
     8581 tgagtatttc ggttccttaa ctagtatagg caacatagat aatctgggta aacgtgatgt
     8641 gggtcacatc agaacagcct ttccttgcga attggccact gttttcggaa gtcaactcgt
     8701 ccaaggttct tttccgcaaa agtgtatcgt gaattatcga ggccgtgtga gatgagtatg
     8761 gcaacgacct ctgttattta cgtcatcagc gacgacccgc gtctcggcga acaggtcgag
     8821 caggctgttg gcgacttgtt ctccgtagag cgactagctt cgtttgatgc gctggagcgt
     8881 ttgggacccg gttattgcac cgtttgcccg gccgttgttg ctcacctcac gaagaagacg
     8941 gtgaatggtt tcccggccaa agagctgctc cagaagctgg ggcaacttga gcggattgct
     9001 cctgttatca ccattggcga cgagaatctc acgaaggaag tgaccgggca ggctaacggg
     9061 gctggccggg tgaattttcc ggcggccttt cgtccagaac agcttcgcca ggcggtggcg
     9121 gaactctcgc ccgtggaatg ggaactggcc gaactctttc attaccgtcc ccatgctgtc
     9181 ctgcggggag ggcatcgcca atgggtgacc ttcacgcctt ccgtcttcga gatggtggaa
     9241 gaacttcgcg tagcggcggc acatgacgtc agcctgctgc tgattgggga aacaggagcg
     9301 ggaaagacct ttttggcaag aacgattcac gagatttcgc cgcgggcagg aaaacgcttt
     9361 ctcacggtag cctgtggtgc cctgccaccg gacctgatcg agagcgaact gtttggctac
     9421 gtcaaagggg cgttcaccgg ggcggatacg gataagccag gcaagtttgc tgccgtggat
     9481 gacggcacgt tactcttgga cgaaatcgac gtccttgccc cggaacacca ggccaagtta
     9541 ctccgggtga tcgagaccgg ggaatacgag cctgtgggaa gcaacgagac ggcgttctcg
     9601 cgggcgcgcc tgattgtggc ttccaacatt gatctgcacc aactcgtgga gcaggggaag
     9661 ttccgtcgcg atttgttcta tcgtcttaac atgttgagct ttttcatccc accgctgcgc
     9721 gatcgcccct acgatattga ctatcttgcc cgaaagttcg ccgtcgaaca gtcccgctca
     9781 ttgtgtgtcc ggttgcgggg gattgatccg gcctttatgg cggcccttca caattaccac
     9841 tggccgggga atgtccgtga actagagaac atcattcggc gggctgtgct gtactgtcgg
     9901 gatggaatat tgcgagtcag cgacttgcca agcatgatgc ggcccgcccc aagagaaaat
     9961 ggcggcgagg ggcttcctga aatttcggtc cgctggacgc ttgataaacg ccttgaggac
    10021 gccgagcggc ggattctcga ggaggcgctc cgccggaatg gatacagacg gcaggatacg
    10081 gcgcgtgaac tagggatcag ccgcatcacg ctgtaccaca agatgaagaa atatggcctg
    10141 atggggaatc atcgcgacga ggtgtccgcc gtgctcgaat gagagctagg gccttaccag
    10201 tatttggacc aggtgctcgc caaagatgat aaatccgatg acggcggccc ccagggatgc
    10261 aaggaggacc gccgcgctgg ccacgtcaag ggccgtctgg atgtaacggt tgtactcttg
    10321 ggtcacggcc ggggcaaacc actccaaggc actgttgatc aactcggccg tcatgacaac
    10381 gccgatgctc aagatgagga tgcaccactc cgtttcggtc acgcccaaga ttgcggcgac
    10441 gacaagaacc aaaaacgcga ccgcaaagtg gactgcgaag ctgctttgac cgacaatgcc
    10501 gtaccacaga ccgcggaagg cagcctgaaa cttttccacc caggaacgcc gtcgtttgac
    10561 cggaggacgg cccatcggta gcccttacat caagcgaaag gtaatacttt tgacgccgtc
    10621 ctaaacttta ggacgccgac aattaagcaa ggttggtttc ccttccaact gtagcttatt
    10681 tcatctgacg ccagaggttc atcccaagag tgggggatac taatggacct aatcagaagg
    10741 ggatgccaaa accgctgtgg gtcccattta gagtctatcc caaaaaaagc cccctctccc
    10801 cccgggagag ggttggggtg agggcaagcg atcgtcggag tcgagttaga aaaccggcag
    10861 cgatttgagt caccctcacc cccgacccct ttcccgccga gcgggcgagg ggagattatg
    10921 ggatgggctc ttagtagaat aaggggttaa tcgcagcaag agaggcgaac accgttcctt
    10981 tggttggtat cattaccccc ctttgttctg aatctgagga gtggggcgat acctgaaagg
    11041 tgggatcgtt tagttgacgc cgcccggcat agacaggcgg ccgttgaaac gatggctaag
    11101 atgcaatcgc tgattcgtag gatccatgaa agtccctgga agttggtcct ggtggccgca
    11161 ggcggcggta ttcagggcat cgccgattta tttcacgagc cgggggcatc gcggacggtg
    11221 ttggaggcag tcattccgta ttccgcggcc gcgctagagg cctggctcca cggtaagccg
    11281 gaccatgcgt gcgcgaaggc gaccgcccgc agcatggcca tggccgcgtt tcttcgggca
    11341 cggcaacttc aacaggccgc gggggagacc gacgactcgc cggtcgccgg ggtggcttgc
    11401 acggcgtcgc tggtcacgga acgtcccaag aaaggccctc accgcgttca tgttgctatc
    11461 cagcgggcag acgtaacgcg atcggtctcg ctgtttctta tcaagggggc gcgaagtcgg
    11521 ttcgaggagg agcaagttgc cagtggcttg ttcctttacg cgatggcgac ggcctgtgac
    11581 ctggaggtgg attgggctct cccgctcctt tccggcgaag tgttggagca cgagaaggcg
    11641 gaggccccga agtcgtggca ggagttaatc ctcggcacct gcgatgttgt tccgctcggt
    11701 gcgccggctg attggcagcg agggcggcct gtggttcttt ttccgggagc atttaacccg
    11761 ctgcacgtgg gacatttgcg gatggcccgc gtcgccgaag cgaagctggg gcggcctgtg
    11821 gagttcgaaa tctccgtcct caatgccgac aaaccgccac tcgattatgt ggagatccgg
    11881 cgacgggtag aacagttcac gttgcaagat cgcatttggc tgacgagact tcccaccttt
    11941 ttggaaaaag cccgccggtt cccgcaggcc acctttgtcg ttgggatcga cacattgatg
    12001 cggattgccg atcctcgtta ttacggacac aatcccgacg cgcgggatca ggccatcaga
    12061 gaattggcgg aacgaggatg ccgttttctc gtttttgggc ggtgctggca gggacggttt
    12121 ttgggcctta aggacgttga cattccagag gacctccgcc gtttgtgcac agaggtgcca
    12181 gagtcagaat ttcgagaaga tatttgttcg actcagttgc gagcacaaga agaatgaaat
    12241 gaaggggcca gggtggctag ccgggtcaca ttggttcaaa ggcatgtttc aaaagagccg
    12301 tccatcttac tcgcggcgac cggttggctg ggacacacgg ccacattatt aatctacgcg
    12361 tgattcggta aagtggatac aatcgccagg gcaatttgtc taaggatgac atttgttgta
    12421 gcctggtgac ggtgaggtat agagactgtg acggtgatag aatccgaggg actcacccct
    12481 tgctgtcgaa gcagcgcgat agtgagcgtc gaacaattga cgaagtatta tgacgatctc
    12541 cgccgtggtc ggattgtggc cgtggatggg atcagttttt ctgcgtcacc aggggaaatc
    12601 ttcggtcttc tcgggccgaa cggggcgggt aagacgacca cattgcggat tcttagtacg
    12661 cttttgaagc caaccgcagg tcgggtactc atcaacggtt acgatggact gacgcagccc
    12721 gatcaggtgc gacgctccat cggttttatg tcggtcaata cagcggttta cgaccgcatg
    12781 acggcctggg aaatggtcga atactttggg atgctgtacg ggctggatcg gcaagagctg
    12841 cgggatcgga tggaacgcct ttttgatcga ctgaagatgc aagagattcg ggacctgttg
    12901 ggttcaaaga tgtccacggg gatgaagcaa aaggtatcca tcgcccgggc ccttgtccat
    12961 gatcccccgg tgttgatctt cgacgagccc acggcgggat tggacgtctt ggttgcccgc
    13021 gcactcctaa ccactattgc ggaactccgc gaccagggta aatgtgtcat tttttccacg
    13081 cacattatgc gggaagcgga aaagctgtgc gatcgcatcg ctattttgca tcggggacag
    13141 attctggcta tgggtaccct ggaggaattg cgagagcaat ccggagagaa tgacttggag
    13201 gaattgttct ttcaactgat caccgaagct gaaagaaaac gagccgaccg ggcgtcgtga
    13261 ggacggattg acgcccgggg cggaaagatg catagcccac tgatgatgac ctggcgcaat
    13321 atctggattg tctttgcccg cgaaatccgc gatcaactca gggaccggcg gaccattttt
    13381 atggtggcgg tcctgccagt tttgctttac cctttgttgg gcatgatatt tttccagctt
    13441 gcccaggtga tgggcgagaa gcccagtcgc atcctcgtca taggggctag agaaccactg
    13501 gagccacctt tgtttgacga aacgggtttt gtcagcagtg tgttcgtttc gcctcaagat
    13561 gcggttctcc tgaaagtgga attcctctcc gacgaggtgc taaaggcaag ggcgggatcc
    13621 gcctccgaag atccgggacg tgcgctcgcc gaagaggccc ttcgcacgga gcattatgat
    13681 gccgtgcttt attttccccc tggttttgcg gcacagttgg ccgactttcg ggagggaatt
    13741 cggcaacaga ttgcggccgg tctgtcgtcc gaggccaatt ccgaagcaaa acccggttct
    13801 gaaaagagtc cggaagaggc aggggaagac gcttcccaaa aacaacagcc cacgccacga
    13861 acggttgttg tcagtcgttc ggcgattcct agtccgaggg tgtattactc cacggcaaac
    13921 gatcggtcac aaacggccta tcgccgattg gtggagctcc tcgatcgctg gacggatcag
    13981 gtcatggtcg cgaatttggc agcagctagg ctccctccag atgccgcgcg accgtttgtt
    14041 tttgaaaccg cggatgtggc agttcagagc ggttttcgcg gtgcggcggt gtgggcaaaa
    14101 attctgccta tactgctggt catgtgggca ttgacggggg ctttttaccc ggccgtcgat
    14161 ctttgtgccg gagaaaagga acgcgggacg ttggaaacgc tcctttcgag tccggctcgg
    14221 cggagtgaaa ttgtcctcgg aaaactgggc gccatcacgg ttttcagcat ggtaaccgcc
    14281 gtacttaacc tgattagcat gggtttgact ggctggcttg cctttgggca cctgccggac
    14341 tttgccgcgc cgcccctttt tgccatcttt tggttggccg tagcattact gcccgtttcg
    14401 ttggtgttta gctgcttggc cttgggcatt gccgcgttcg cccgcagtac caaggagggt
    14461 caatattacc ttttgcccct tttgttttgc accttgcccc tggtgggtgt gccgatggca
    14521 cggggatcgg aattggattt gggcactgcc cttatgccca ttacaggctt ggtccttctc
    14581 ctcaagacga tgatcgaagg tcaaatggcg gaggcactac gctttgtgtt ccctgtagga
    14641 ctggtaacct taggcggatg ctactttgcc ctgcgttggg cggtttatca attcaacagc
    14701 gagacggttc tgtttcggga aagcgaacgc ttccacctgg tcgcttggct tcgccacatc
    14761 tttcgccaac gggcagtgcc cagcggcgcc gccgccctga catgtgggct ggccatcctg
    14821 ctgatgcgat ttcttttgac gaacatcctc acccgcattg gcgtctcgtg gaatctcgtc
    14881 cagcaggcct tgatttcgca agtggtagta atcctcttgc cggttgtcgt tctagcagcc
    14941 ttattgacgc gggaaatccg cgcgacgttc ttgctccgac tcccgtcctg gcgtgcggtg
    15001 atcggagcgg tcctccttgc cgtaatgctt catccggcct tttttacttg gcaacaagtg
    15061 atcgttcgtt tatatccgct tgatcctgta ttgaaagagt ctctcaagca attaaccggg
    15121 caggcccttg atttgcggca gctccttttg gtcatggccc ttttgcccgc catttgcgag
    15181 gagttggcct ttcgtgggtt tattctgacg ggatttcggt cctcgcttcg accgacgcat
    15241 gccttgatct tcgcagcgct ctttttcgcc ctgggacaca cgatcttaca acagtccatg
    15301 gtggcctttg cggcagggct tatcctcggt gtgattgcca ttcaaacggg gagcctgctc
    15361 ccttgtgtta tctatcatgc aattcacaat gccttgctca tcctccaggg aagtctgggc
    15421 caaagccgat gggtcagtga tccctggatc aaagccatga tgacctggac agatgaagga
    15481 cctacctatc acttgtcggt agtggtgttt ggcatcttgg ccgccggact gatctttgcc
    15541 tggatgatct cgcgcccgga ggtgcttcgc tcctatctgg ccgaagaaga gaccggctcc
    15601 cattgagtga gacaatcccg ccatgacgag tgccgccgat ctttgtgtga ctgccgattg
    15661 gattcttccc cttgacggac cgccgatccc caatggaagg atcaccattg ctggtcaccg
    15721 tatcgcggag atcgatgccg gcggcgaacg cgttcggttg atgcgccatg acccgcgtcc
    15781 acagggcgta acgcatcttc attacaagga ttgcgtcatc cttcccggcc tgattaacgc
    15841 gcatacacat cttgatctca gcggatggtc gggcgatttc atatcgccac ccaaactttg
    15901 ggattggatt cccgaggtcg tccgttttcg taggtcaaat gcatatcggc ctggggaagc
    15961 gatccgggat ggccttcagg ccgtgctggc aggcggagcc gtcggtgtgg ttgacgtgat
    16021 cccgcccgat tggtttacct ggggtttggt tcctgaacag ggcgcggacc ctgacggtga
    16081 tcagcaaatg cccgagtggg aacccagcag gctacgggtc ctcagtatga ttgagtgttt
    16141 ggcgccaacc gggaggatgc ggattccgcc ggaggagctt ttacaacacc acgcgatggc
    16201 ttgccgccaa atgggctggg agattgggct tagcccccat gccccttaca cggttcccat
    16261 gagcgtcttg aaagaggtcg tgaaagtggc ggtagatcac aatgcaaccc tcaccatgca
    16321 catcgcggag acagaggagg aggccgtgtt cctcagcaca ggaggcgggg ccttccgaga
    16381 catgctgaag catctctcgg cttataggga cggggtcttc tccggcggaa acagcctcca
    16441 ggacgtgctt gaggtccttg tgcaagcgcc acgcgttctt ttgattcatg gaaattattt
    16501 gggccggtct cagattgact ttctatgtca gtttcgggac cgggtggccg tggtctattg
    16561 tcccaggtcg cacgagtttt ttggtcacac cccgtatcct ttggagcagt tcctggaggc
    16621 gtcgattccc gtccttttgg ggaccgacag tcgtgcctcg catccggata tttccatgtt
    16681 aggggaattg caggccgttc ggcgaaaaca cccacatatt gaggcagaga cgaatatccg
    16741 catggccacg tcaacggctg ccaacgtctt gggctgggag aacgacttgg ggaacctcgc
    16801 tgtagggcgg ctggcgagct tcacggtcgt ccatgtggat ggtctcgcat caaaaaagga
    16861 cccctacgaa gcggtgcttt ctccgcatgc ccaggtttgt gcggtttggc tggcaggcaa
    16921 gctcgcgtat cagcagacgg gccatgcggg tgagcaagag ggttccgtgg catgaaccat
    16981 gggcgacgat tggacattcg cgattgcgtg tggattgtcg gtccgacggc agcgggcaag
    17041 tcggaggtcg gtgtcgctct ggcggagcta atcggcggag aggtgatttc cctcgattcg
    17101 atggccgtct atcgggggat ggacatcggc acggccaagc cgacggtgga gcagcggcaa
    17161 cgtgttacgc atcacctcat tgacattgtg gacccttggg aagagtacag cgtcgctcgg
    17221 tatcttgcgg atgcggcaac ttgtttggaa gacattcgca ggcgaggtaa aattccgctt
    17281 tttgtcgggg gcacaccgtt gtatcttaag gcggtcctac gaggtctgtt tgaaggccca
    17341 caggctgatt gggagttccg tcagcattgg cgcgcactcg ccgagacgaa ccctcctggc
    17401 tttctgcacg ccgaagtggc gaaagtcgat ccggcaagtg ccgcaagact tcacccccat
    17461 gactggcgga gactcattcg cgctctggag gtttatcaca agacgggtcg tcccttaagt
    17521 gagtggcagt ctcaattcga caagcctcgg gaagaagcta tggggcgggt cgttgtgctc
    17581 gattggccgc gcgatattct ttatcgccgc atcgaggccc gcgtcgatgc gatgtttgac
    17641 cgcggcttgg tggcggaagt gaaacgcctc gtcgcggatc cgcgggggtt tggtcggacg
    17701 gctcgtcaag cgctgggata tcgagaagtc ttagactacc tagcgggcaa gatgagtctg
    17761 gaggacacaa ttaaccttgt taaaacgcga acacgacaat ttgccaagcg ccaactgacg
    17821 tggttccgtc atttagagga atgccgcaat ctgccgatca acgagccatg gcagcccgag
    17881 gacttggcac gtcgtattgc ccgcgatctt agtttgacac cactttcttg accacctaca
    17941 atcgggttaa tggggattcg gcaaccgatg ttatgtggaa agatgaggtc tcgatgatga
    18001 agaatgagcg gttgaaaaag gaagagctcg agtactatca caagctcctt ttaaggcttc
    18061 gtgagcggtt ggtgggaaat gtgaagtact tgaccgatgc cgccctgaag caggcgcagg
    18121 atgtggcagc aacgcgggct ggctccactt tgcatccggc cgaggcggga agcgacaatt
    18181 ttgaacagga gttcacgctc tccctggtgg aaaacgaaca cgcggtcttg gaaggcattg
    18241 aggcggcgat caaggccatc gaagaaggga catatgggat ttgtgaggtt tgtgggtgca
    18301 ggattcccaa agcgcgcctc caagcgttgc cctacgccac gatgtgcatt aagtgcgccg
    18361 agaaaactga gaagggccaa gcctgatttt ctacccgcct gattttccca cctcaattca
    18421 cttccttgcc tgtttgtgag ttccacttcc aacccaagag aaggacaaga aaaaaacaag
    18481 cccgattgtc ggttattcca acaatcgggc ttcaccttgg aggccaaaag cgcggtgacc
    18541 atcgggacgc aatgatcacc gcagctaaag tttcatgctc gaggtgatat tgccccttat
    18601 cagaggacgc cctgggcgag catggcatcc gccacccgca caaaaccgcc aatattggcg
    18661 ccatccacat aatcgacgct gccatcgggc ttcgcaccat agcggcagca tgtctcatgg
    18721 atgttttgca tgatctgctt gagccgtcga tcaacttcct cgcgggtcca ggaggtgtgc
    18781 tgggcgtttt ggctcatctc caagccggag acagcgacac cacccgcgtt agccgccttg
    18841 cccggtgcga acagaatctt cgcccgtcgg aagacctcga tggcctccgg tgtcgagggc
    18901 atgttggcac cttccgaaac acactggcaa ccattcttga ccaacgtttc ggcatcgtca
    18961 ccgttcagtt cattttgggt cgcgctgggc agcgcgatgt cgcaggggac accccagggc
    19021 ttttggttcg ggtagaactc gcacttgaac ttttccgcat actcactgat ccggccgcgc
    19081 cgttc
//
LOCUS       DAOSUT010000077        18444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1908_length_18444_cov_9.378977, whole genome shotgun sequence.
ACCESSION   DAOSUT010000077 DAOSUT010000000
VERSION     DAOSUT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 18444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18444
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_18444_cov_9.378977"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLD05_08720"
     CDS             <1..687
                     /locus_tag="PLD05_08720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP77562.1"
                     /translation="GIGEAIAKVLAQEGYIVAILGRTESKLRRVVEDIQAIGEAAYYV
                     CDVSDRTRVDQVFADIAQKMGAPRLLVNSAGINVVNRSMATLAPEDWDRVLAVHATGS
                     YNCIRAVLPAMRDAGEGLIINISSIAGKRALELAGAAYCAGKFAMTALGMAAGLEERH
                     HGIRITNIYPGEVNTPILEQRPEPVPQERREQMLQPEDLAEIVRTIVRLPSRAHVWEV
                     VVTPLYQPYS"
     gene            726..938
                     /gene="yacG"
                     /locus_tag="PLD05_08725"
     CDS             726..938
                     /gene="yacG"
                     /locus_tag="PLD05_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOP77563.1"
                     /translation="MKKIRCPVCGRMFDPETTEAMPFCSRRCRIIDLGRWLGEQYAIP
                     HVEKDEEDEDSETPRLSDHESPEDYY"
     gene            1319..>1962
                     /locus_tag="PLD05_08730"
     CDS             1319..>1962
                     /locus_tag="PLD05_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77564.1"
                     /translation="MPDASTVKDPHFNPLCAWLGFPPDALPCDYYALLGLPRFESDTG
                     EINRAAETLIAHVREIRPGPYLAEWTQLLDTIRAAKACLCDPEKKQAYDQALRTRRIT
                     TLDVNEWSQSRVLTADVDNGPEPSTAHVAAQALQTSPSLSRHTTSVGPTANTETVAPS
                     ISLKHFPTRKKPNWLAQIFSVFFLLVSVILAYQMLTAPAWWGARLRVILAAAQLG"
     assembly_gap    1963..2062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2063..2743
                     /locus_tag="PLD05_08735"
     CDS             <2063..2743
                     /locus_tag="PLD05_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77565.1"
                     /translation="PNHIAELPNNEVEFPNNEVQMPTFQEKQGEEPHEMSPSVESPTD
                     YEAAPPDLRPEIRSGVVHVWQAMAKRNLEAARSKLDQLAGQVTNEAEQQAVEVLDYLL
                     KHVEEFWRALHKIVSTMQAGEEFAIGDAYIVVVEATSEELVIRAAGQNRRYLIRQMPS
                     PLIRLIVSRRFRPGPEADALLGAFLAVDPEGSPQEAEQLWRRANDGVLDVEALLEALK
                     LRPEASSR"
     gene            complement(2909..4381)
                     /locus_tag="PLD05_08740"
     CDS             complement(2909..4381)
                     /locus_tag="PLD05_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77566.1"
                     /translation="MNCRTASKCCAPGVFRQLAQRHLEGLGRKVGLPTLLTICLLGYW
                     SGMTPAVLAEGPQDPTAVSWDYSTAYRMAEEKRGLLVVAFVKPGQEGSAKTFVQSLMG
                     DSRLRGWLAAAVVVTGSPVQIRGEAIVLSDHAAFAPLKGQPGLAVINFGVDPNNDEYG
                     RVIWALQVKDEEWDGYVELMRVSLVELAVAAGIGQDVKRATVNLPERIPNNTQENDGV
                     VAAAYLAPEQSSIWMTDYKKAVEKARNEKKMLLVYVRPQQPDQQVLQFERKTLLDPLV
                     MKELFKFVCVRVSEDYVIEENETKIKILDHPSLVEMANRPGLFIVDYASTDAEYYGQV
                     VSVFPFLNGRPYDVDQMGVILTLPPGTLTQRTMIYAVRVHPERPKSILGRPEPFLFKE
                     AEKHSAYQARIRLLGHHFWETRFHRILQVLRNESTASEVCAQSWPGQRLLEAALECVR
                     CWRLSPGHWRAVSSYQDAYGYDIKKGSDGIWYATGVFANH"
     gene            4688..6553
                     /locus_tag="PLD05_08745"
     CDS             4688..6553
                     /locus_tag="PLD05_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77567.1"
                     /translation="MATLLRFNVTCLVISAMALGLFVMPSRLGLANSPESQPDWPTLC
                     QQAVAEFREITPQDVQRARDELLFAVKVLDARLDRAGPEAEGWRDYLLWRAFSEELKK
                     DSPSLDVLGAVYQRLSAGHEGLGLTWFRRVRLTLERFLRLSQAVTDKNAQANYQTVLN
                     ELGKSLAAYESDASPETATRVQELLRWLETMGQAKSARDAVLNHFSHPNVTIRVSGHW
                     ISISSQARVDVREPVREWILGTDVRGTGHMVGWRKLQIIPCEQHALFAIRVEGVLNSD
                     TIGYNGPARIYSHSKTPFVSQKLIQFGPDGLTAQPARCDASVSTDIQDVDVTCRSQCV
                     EKIAWRRTFQQKPAAEREAAWKAERRIARRVDQEADERLAKAQSEYLQKIREPLADRD
                     LFPDLFLTKTTADAFHVTIRQMGHSGLAAGTSAPQAPEGAVVVQVHQSFVNNFVEGFF
                     GGMVLKEARLQEAVRDLLGELPKELESDPDGEPWTIRFSEHRPLAVRFADNQIAIEFR
                     GTTYWKGDRNYPGMDVKAAYRIESLDDGKSFRLVRQGPLAVFPPNFDPSTDRLSVRQQ
                     TIRRLLERRFEKIFRDTIELEPIKLSGDWEKGGPLAVKSIASQKGWLTVALDQIQ"
     gene            6808..7302
                     /locus_tag="PLD05_08750"
     CDS             6808..7302
                     /locus_tag="PLD05_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77568.1"
                     /translation="MTEHVEGPRWVDITFDCVPLRMVTRSDPPLDASPGFLALWDRVQ
                     EAIRRFGRHNSYYLFNGQCTFHLTNNPSKGTLSFAFEGCLLTDSEDKVATAFDVHTTL
                     RGDTCDWATEPIIRWFAESVREAVLVEFNHFIAHGDLEKTIQRLQKLEQEMEAKGGYV
                     GFGL"
     gene            complement(7281..8381)
                     /locus_tag="PLD05_08755"
     CDS             complement(7281..8381)
                     /locus_tag="PLD05_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOP77569.1"
                     /translation="MWHGIKGHDENVERFRRAVSRGRIGGTYLFLGPPGIGKKLFALK
                     FAQTLLCEKHPAELFDPCEACASCLQVISRTHPDVMFVSRPPEKSFIPLETLIGERGQ
                     RNVEGAGLIGHLALKPYSGRFRIGIIDDADYFNAEGANALLKTLEEPPPQALLILIGT
                     SASKQLPTIRSRSQIVRFRPLPVDILTELILEEGLSQNLDEARRLAQLSGGSLERARD
                     LADPEMWPFWVRWRAELSGISFDAVTWSQEFRTFVDNAGKEAAARRKRLYHILSFTAN
                     FLRDAITLLVGGHADDSTSAECQNFVRVIKEILGPDAVEVIQHCVETCLQACEDIERN
                     ANLTILIDDWITTLWHCLNRSRVASPQSPKPT"
     gene            complement(8550..9164)
                     /gene="tmk"
                     /locus_tag="PLD05_08760"
     CDS             complement(8550..9164)
                     /gene="tmk"
                     /locus_tag="PLD05_08760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOP77570.1"
                     /translation="MFIAIEGADGTGKTTQVERLCRWLEEKGHKVVRCRDPGGTALGE
                     AVRDILLHRQAVPLDARAEALLYMASRAQLVAEVIRPAVEAGCIVVSDRFVLSNIAYQ
                     GYGFGLSVETLRQIGDFATAGVQPDVTIVLDMPVAQALQRIQRTPDRLESRGVEYLER
                     VRDGFLQEATRYADRVIVVRADRHPDEVEASIRNIVEPFLGQPK"
     gene            complement(9511..11895)
                     /locus_tag="PLD05_08765"
     CDS             complement(9511..11895)
                     /locus_tag="PLD05_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOP77571.1"
                     /translation="MKTSRGLLGITAWALITLYLCGCRPQQPFFLHEDGDLSHYVDKA
                     TQIEYPDVEAESLDEVQKASPPLTLSNSDVKEYWDLSLEEAIRIALQNSKIIRTLSQP
                     DVGMPRLPETSLTVAPEGTPTVYDPARQETNPRTGVEGVLADFDAHWSTRFFWQKSDT
                     PQNVAGFVQAFRPQAFRQDTGNFETEISKTYATGGTFSISHSVGYDWNNAFTSRQWSS
                     HWTANVEAGFRQPLLQGAGVTFNRIAGPNSIPGFYNGVLIARLETDQRLAEFEQSVSE
                     LLLDVEKAYWTLYLSYRQLDTVIAGRDAALQTWRQVRAKYEVGGRGGSAQEEAQSRQQ
                     YLAFRAAVEQALSSLYKAENNLRYLMGLTATDGRLIRPKDEPTRARVEFDFHDVHAEA
                     LIRNPLLRREKWRVKKAELELIAAKNYLLPKLDMFGFYRWNGLGHDLIDPDDDRSNAY
                     GSLTSGDYQDWQLGMELQIPIGFRKELAAVRNAQLNLARERAILQEQELEISHQIAWV
                     LREIDEYYTLAQTNYNRTVAAENEVRAVTAMYDAGTATLDLLLNAQQRRAEAEIQYYQ
                     SLTKYMFAISHLHFRKGSLLEYNGVYLTEGPWPQKAYFDALRRARARDAGLYLNYGMT
                     NPPVFSRGPYDLHAGKDGAEELLIDGLPDGVEIDQQGAPSVIELEPPPMPSEDMPGST
                     SDASKTEQAAEQKDASRQTRREDWTTSVPQQRRASTDDGSSAAISRKQDQLVALASHV
                     SYTSRTSSTPSAPSPSRNQPSEQGTGDAPSSPVQATHTNVSRQTTDNSGWRARR"
     gene            complement(12275..12706)
                     /locus_tag="PLD05_08770"
     CDS             complement(12275..12706)
                     /locus_tag="PLD05_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOP77572.1"
                     /translation="MVWIQKELQLPRFGKGFHAITRHIVEALPELKSIRVGLLHIFIQ
                     HTSASLTINEAADPDVADDLHQVFDRLAPEDLPYRHTVEGRDDMPAHVKTTLSDCSLI
                     VPVRNGRLALGTWQGIFLCEHRRNNSGRHLILTLYGEKEPT"
     gene            13071..13304
                     /locus_tag="PLD05_08775"
     CDS             13071..13304
                     /locus_tag="PLD05_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77573.1"
                     /translation="MSCKHLQELYQLCHQYNLKLTGSELVRIVCPTCGVDEICPNVLM
                     EEYDRRHSPEEAPPTSADETKVTSPDQVKGSSE"
     gene            13589..13762
                     /locus_tag="PLD05_08780"
     CDS             13589..13762
                     /locus_tag="PLD05_08780"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOP77574.1"
                     /translation="MTAIVNQQTCNGCGECVQACPLDAIKIENEKAFVDPDVCGDCGA
                     CVDVCPTQSISLS"
     gene            complement(14141..14842)
                     /locus_tag="PLD05_08785"
     CDS             complement(14141..14842)
                     /locus_tag="PLD05_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP77575.1"
                     /translation="MRCLDVVTPFLLVLPLSIVCAQTPGEDNVPPPCFVALFNGKDLT
                     GWKGLVGNPVTRAKMTPEELKRAQEVADQRMREHWKVENGVLVFDGKGDNLCTVKDYG
                     DFELLVDWKILPHGDSGIYLRGSPQVQIWDATDHPEGSGGLYNNKIHPSKPLKCADKP
                     VGEWNRFRIRMVGERVSVWLNDELVVDNVVMENYWERDKPIYPTGPIELQNHGNTLYF
                     KNIFIRELPRDGESP"
     gene            complement(14932..16410)
                     /locus_tag="PLD05_08790"
     CDS             complement(14932..16410)
                     /locus_tag="PLD05_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77576.1"
                     /translation="MNRSPSMRFFRRVLPGILLVSLGLRVSSQIWGGETEERPNILFA
                     IADDWGWPHAGAYGDPVVQTPTFDRIAREGVLFTRAHVIAPTCTASRGGILTGQAAHR
                     LEEGANLHSRLPAKFRCYPDILEEAGYAIGIMRKAWGPGVLEGTGRQRNPAGPQFRNF
                     EEFLKTVPDGRPFCFWFGSTDPHRPYEKGSGVASGMDLNKIRVPPFLPDTPVVRSDIA
                     DYYFEVQRFDRELGEVIKLLEATGRLDNTLIIVTGDNGMPFPKAKATCYDAGTHVPLA
                     VCWKAKVPGGRVVDDFISLTDVAPTILEAAGLKPLPEMTGKSFLNVLLSHKSGRVDPS
                     RDHVFTERERHTCTRPNNASYPIRSIVTERWHLIWNLRPHLWPAGNPPEESVGRDYGD
                     IDGSPTWTEMLQRQNDPAIRPFFLAAGGAKRPEFELFDLLADPGEMNNLAGKPEVAAI
                     EKELKEKLQAWMRDTGDPRASGDTDFWDRAPYYGDRPKPKTP"
     gene            16503..>16789
                     /locus_tag="PLD05_08795"
     CDS             16503..>16789
                     /locus_tag="PLD05_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77577.1"
                     /translation="MNYFAHAVRFLDRPYFLAGTSLPDWLSVVKRTVRLRRTSLTEHL
                     TSPDERTRQLVAGVVQHLEDDYRFHSAPAFSEVLVQVLRLIQPVVNTPGVS"
     assembly_gap    16790..16889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16890..17069)
                     /locus_tag="PLD05_08800"
     CDS             complement(<16890..17069)
                     /locus_tag="PLD05_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77578.1"
                     /translation="MIHLRLSNAYFSQKAAHTKAKRLAAKRTRQKPSPYSAKFTLSRL
                     APAKDRAPSPPRSTGY"
     gene            17068..17922
                     /locus_tag="PLD05_08805"
     CDS             17068..17922
                     /locus_tag="PLD05_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP77579.1"
                     /translation="MRFGLNLLLWTDTLCDEYLPLLDELKEIGFDLVELPVFDLDNDA
                     NYKKWAKRLDELGLARTATAIRTAEENLISSDPAVRRKGIEANKRNLDCCAMLGAEIM
                     AGPFHSALGVFSGRGPTEDEWKWAVEGMREVAEYADKVGVTLAVEYLNRFECYFLNTA
                     ADGARFCRDVNHPRCKMMYDTFHSHIEEKNIPQAIRALKDYLVHVHISENDRSTPGTG
                     NVRWPETFDTLHEIGYNNTMVIEAFGLALDKLVPATKIWRRMYESERQLATDGLRFMK
                     TEVAKRWK"
     gene            18272..>18444
                     /locus_tag="PLD05_08810"
     CDS             18272..>18444
                     /locus_tag="PLD05_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP77580.1"
                     /translation="MFARKPRGFTLVELLVVIVIISVLVALLLPAIVGSRESARRAQC
                     QHQLSQLGQALQT"
BASE COUNT         4144 a         5123 c         4844 g         4133 t
ORIGIN      
        1 ggaattggcg aagccattgc caaggtcctg gcccaagagg ggtacattgt cgcgatcctc
       61 ggtcggacgg aatctaagct acgccgcgtt gttgaagaca ttcaagcgat tggtgaagca
      121 gcctactatg tgtgtgatgt cagtgaccgc actcgggtgg atcaagtctt tgctgacatt
      181 gctcaaaaga tgggtgcgcc acgtttactg gtgaatagtg cgggcatcaa tgttgtcaat
      241 cggagcatgg ccacgctcgc tcccgaagat tgggaccgcg ttttggccgt gcatgccacg
      301 gggagttaca actgcattcg agcggtgtta ccggctatgc gggatgccgg cgagggcctt
      361 attatcaata tttcctccat tgccgggaaa cgggccctgg aactggccgg tgctgcttat
      421 tgtgcgggta aatttgccat gacggccctc ggaatggcag ccggtttgga ggaacgtcac
      481 cacggaattc gcatcaccaa tatctacccc ggcgaagtaa acacgcccat actggaacag
      541 cggcctgaac ccgtaccgca ggaacgccgg gaacagatgc ttcaaccgga agacctggct
      601 gagatcgtgc ggacgattgt gcggcttcca tcgcgagctc acgtgtggga ggtcgttgtc
      661 acaccgctgt accaaccgta ttcataagga agacaccgtc ggcttgagaa tcctttccgc
      721 aagctatgaa gaagattcgt tgccctgttt gtggcaggat gttcgatccc gaaacgaccg
      781 aggccatgcc tttttgtagc cgtcggtgcc ggatcatcga tcttggccgg tggctaggcg
      841 aacaatacgc gataccccac gtcgaaaaag atgaggagga tgaggattca gaaacaccgc
      901 gtctctccga tcacgaatcg cccgaggact actactgact tggcaattgt ctttgggtta
      961 tagccgtgat acaggtaaga gtataaagcg ttctggccgt ttttggatgt ataacccgcg
     1021 gacgcccagt tttcacgagg agccttcgct ttcaagagag agttgtgata tactttttcg
     1081 ccccaatcca gcttgactcg caggaaagac aggaaagaag tgatcgccaa catctcagta
     1141 ccacgggggt tcccttggca gagcgatccg ccacgttcat gccaagcaaa gtcacgctag
     1201 aattgaaata agtgagacca gcgtcagttc ggaactgctg ggcagttgtt ggttccccga
     1261 ttcccttaaa gggtcagttg ccatcagtga ttcgccaatc attcaaatgg ggttgtgtat
     1321 gcccgacgct tcgactgtaa aagatcccca ttttaaccct ttgtgtgcct ggttgggctt
     1381 tccaccggac gccctgccgt gtgattacta tgcactcttg gggcttccgc gatttgaaag
     1441 tgatactgga gaaattaacc gagcggcaga gaccctgatt gcccacgtgc gcgagattcg
     1501 ccctggtccc tatctcgccg agtggaccca acttctcgac acaattcggg ccgccaaagc
     1561 gtgtctttgt gatcccgaaa agaaacaagc ttatgaccag gccctcagga cccgtcgcat
     1621 taccacactt gacgtaaatg agtggtctca gtctcgtgta ctgacggcag acgtggacaa
     1681 tggacccgag ccttccaccg ctcatgtagc cgctcaggcc ttacaaacat cgccatccct
     1741 atcacgacac accacatccg ttggtccaac ggcaaatacc gagactgtcg ccccaagcat
     1801 atcactaaaa cacttcccca cgcgaaaaaa accaaattgg ctagcgcaaa tcttctcggt
     1861 ctttttcctt ttggtttcgg tcattcttgc ctaccaaatg ttgacagccc ctgcgtggtg
     1921 gggagctcgg ttgcgggtaa ttctcgccgc agcgcaactc ggnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnccgaacca tattgctgag ctcccgaaca acgaagttga
     2101 attcccgaat aatgaagtcc aaatgccaac gttccaagaa aaacaggggg aagaaccgca
     2161 cgaaatgtca ccatcggtag agtcgcccac tgattacgaa gcggctccac cagacctacg
     2221 accagagatt cggtctggcg ttgtccacgt ttggcaggcc atggcaaaac gcaatcttga
     2281 ggccgcccgc tcgaaattgg atcagcttgc cggacaagtc accaacgagg ccgaacaaca
     2341 ggcggtggaa gtgctcgatt atctcctcaa acacgtggaa gagttttggc gggcacttca
     2401 taagatcgta tccacgatgc aggccggtga ggagtttgcc ataggggacg cttatatcgt
     2461 ggttgtggag gccacttcgg aagaactcgt gattcgtgcg gcaggtcaaa acaggcgcta
     2521 tcttattcgg cagatgccaa gcccgctgat tcgcctgatc gtctcacgga gattccgtcc
     2581 cggcccggaa gccgatgccc tgttaggtgc ctttttggcc gtggatcccg agggtagccc
     2641 gcaagaggct gaacaattgt ggcgacgagc taacgacggg gtactggatg tggaggccct
     2701 cctcgaggcc ctgaaactgc ggcctgaagc ttcgtctagg tgactgcctc aataacgcgc
     2761 tgctatttcc cctttcgcct caaggcccgc aacgtgagca agcaggtctt gccgaaagtg
     2821 acttaaaaac tctggctcct agcatgaaac agccgccaga acgccctccg cacttctcgc
     2881 tgtggtttgt gggcataagg aaaaaacatc agtgattggc aaagactccc gtcgcgtacc
     2941 aaataccgtc ggaacctttc ttgatatcgt atccgtaggc atcctggtaa gacgacacgg
     3001 ctcgccaatg tcccggtgat aatcgccagc agcggacaca ctccaacgct gcctcgagga
     3061 gccgctgtcc cggccaactt tgggcacaga cttcgctggc cgtggattca ttgcggagca
     3121 cctgaaggat tctgtggaaa cgtgtctccc aaaagtgatg acccagcaat cgaatccgtg
     3181 cctgatatgc ggagtgtttt tctgcctctt tgaaaaggaa tggctcgggt cgccctaaga
     3241 tgcttttcgg ccgttccgga tggactctta cggcataaat catggtccgc tgggtcagtg
     3301 tccctggcgg caacgtcaaa ataacgccca tctggtccac gtcataaggc ctaccattga
     3361 ggaacggaaa gacactcaca acctggccat aatactcggc atctgtcgaa gcgtaatcta
     3421 cgatgaaaag gccgggacgg ttggccattt cgaccaggct ggggtgatcc aggattttga
     3481 tcttggtctc gttctcttcg atgacgtaat cctcggaaac ccggacacaa acaaacttga
     3541 aaagctcctt catgacgagg gggtccaaca atgtcttccg ttcaaactga agaacttgtt
     3601 gatcaggctg ctggggacga acgtaaacga ggagcatctt tttctcattt ctggcctttt
     3661 ccacagcctt cttgtagtcg gtcatccaga tcgaggactg ctccggtgcc aaatatgctg
     3721 ctgccacgac accgtcgttt tcctgcgtat tgttgggaat cctttctggc aggtttaccg
     3781 tggccctttt aacgtcctga ccaatgccgg cggcgacagc taactccacc agggaaaccc
     3841 gcattaactc aacatagcca tcccattctt catcttttac ctggagtgcc caaatcacgc
     3901 gaccatattc gtcattgttc ggatcgacgc cgaaattgat cacggccaag cccggctgtc
     3961 ccttcaacgg agcaaatgcc gcatgatcac tcagcacaat cgcctcaccc cgtatctgta
     4021 ctgggctccc ggttacgacg accgctgcag ccagccaacc tcgcagacga gagtcgccca
     4081 tgagcgattg gacgaacgtc tttgcgctcc cctcttggcc gggtttgaca aaagccacga
     4141 caagaagacc ccgtttctct tccgccatcc gatatgccgt ggaatagtcc cacgaaaccg
     4201 ccgtggggtc ttgaggcccc tccgcgagaa ctgcgggagt catcccgctc caatagccga
     4261 gcaagcatat cgtaaggaga gtgggaaggc ctacctttcg ccctaacccc tccaaatgtc
     4321 tctgtgccaa ttgccgaaaa acgccaggtg cacaacactt cgatgcagtt cggcagttca
     4381 tcgcaggtac tctccagaag caaagccttc acatgactga ccaaaaccgg gccgtcccag
     4441 aaagtccatg agcagccatt gtgtgtgaat ctacattaaa tgcacagtct gtatcatttg
     4501 caaggcgcat aacgattgtg ggctatcgga aaatggcatt gtatttagcg actcggcaaa
     4561 gtaaaacatt tgagaaacat attcgtgcca acggtccaaa cggctgatgc ccagcggtcg
     4621 gactacggtg gaagcgcatg ccgtgggatt gggccgggca gcatttcgcg cattggaggc
     4681 tcgtacaatg gcgacactct tacgcttcaa cgtcacttgt cttgttattt cagcaatggc
     4741 cctgggccta ttcgtcatgc cttctcggtt ggggttggcc aacagtcctg agtcgcaacc
     4801 ggactggccg acattgtgcc aacaagcagt ggcagaattc cgtgaaataa cgcctcagga
     4861 cgtgcaaagg gccagggacg aacttttgtt cgccgttaaa gtcctcgacg cccggctcga
     4921 tcgggcagga cctgaagcgg aaggctggag agactacctg ctttggagag ccttttccga
     4981 ggaactcaaa aaggattctc cgtctttgga tgtcctaggg gccgtctatc agcgactctc
     5041 ggcagggcat gaaggtctag gactaacctg gttccgccgg gtgcggctga cccttgagcg
     5101 ttttctccgt ttatctcagg ccgttacgga caaaaatgcc caggcaaact accaaacagt
     5161 ccttaacgag ttgggaaaat ccttagcggc ttatgagtct gatgcttcac ccgaaacggc
     5221 caccagggtt caagagcttc ttcggtggct agaaacaatg ggccaggcca agtcggcgcg
     5281 agacgccgta ctaaaccatt tctcccaccc taacgtcacg attcgagtca gtggccattg
     5341 gatcagtatc agttcccaag cgcgtgttga tgtccgagaa cctgtccgcg aatggatttt
     5401 gggcaccgat gtgcggggta cgggacacat ggtgggatgg cgaaagcttc agataattcc
     5461 gtgcgaacag cacgccctgt ttgccatccg ggtggaaggg gttctgaact cagataccat
     5521 cggctataac ggtcccgccc gcatttattc gcattccaaa acgccgtttg tctctcaaaa
     5581 gttgatccaa ttcggtcccg acgggttgac cgcccaaccg gcgcgttgcg atgcctcggt
     5641 ttccacggat atccaagacg tggacgtgac atgtcggagc caatgcgtcg aaaaaatcgc
     5701 ttggcgacgg acgttccagc aaaaaccggc ggcggagcga gaggccgcct ggaaagccga
     5761 gcgacgcatc gcaaggcggg tcgatcagga ggccgatgag cgcttggcga aagcgcaatc
     5821 ggagtacctt caaaagattc gcgaaccgct ggcggacagg gacttatttc cggacttgtt
     5881 tttgacaaag actacggcgg atgccttcca cgtcacgatt cggcaaatgg gacattccgg
     5941 cctggccgcg gggacatcgg cgcctcaagc accggaaggg gctgtcgtag ttcaggtcca
     6001 tcaaagcttt gtgaacaact ttgtggaagg attttttggg gggatggtcc tcaaagaggc
     6061 ccgattgcag gaggccgtcc gcgatttgct gggcgaactc cccaaggagt tggagtccga
     6121 ccctgatggg gaaccgtgga cgatccgatt ttcggaacat cgccccctag ctgttcgctt
     6181 cgcagacaat caaatcgcca ttgagtttcg gggcacaaca tattggaaag gtgatcgaaa
     6241 ttaccctggg atggatgtca aagcggccta ccgcatcgag tccctggatg atgggaagag
     6301 tttccgacta gtgcgtcaag ggcctttggc cgtttttccg cccaatttcg atccctcgac
     6361 cgaccgcctc tccgtgcgtc agcaaaccat tcgccgattg ctggagcgcc gatttgagaa
     6421 aatctttcgc gacacgatcg agttggaacc catcaagttg agcggggatt gggaaaaagg
     6481 cgggcctttg gcggtcaagt ctatcgccag tcagaaaggt tggctcacgg tcgccttgga
     6541 tcagattcaa tgacaaatgg caatagccct tcggaaccgg gtctcgtcac cgtgggctgg
     6601 actgccacga tatgattgaa agtagctact gcggccagcg tccaaggtag ctccaagacc
     6661 aattcgcttg gccaggttgg cccttgcgac cgctgcggtt tacctcgatg gataacaccc
     6721 gttaaatcca aatgcgaaac gcggtacacg aattgtgttg tctggggtat ttccaactga
     6781 cttcttactg tctgggatgg ctttttcatg actgaacatg tagaaggccc tcgatgggtg
     6841 gacatcacat tcgattgtgt gcccctgcgg atggtcacgc gatctgatcc gccgcttgat
     6901 gcctcgccgg gtttcctcgc cctttgggat cgcgtccaag aggccatccg aaggtttggt
     6961 cggcacaatt cctactacct tttcaacggc cagtgtacct tccacctgac aaacaatccc
     7021 tccaagggca cattaagctt cgcctttgag gggtgcttgt tgactgattc tgaagacaaa
     7081 gtggcaacgg ctttcgatgt ccataccacg ttgcgagggg atacctgcga ttgggctacc
     7141 gaaccaatca ttcgttggtt tgccgagagc gtgcgggagg cggtcttagt cgaattcaat
     7201 cactttattg cccacggaga cctcgaaaag actatccagc ggctgcaaaa gctagaacag
     7261 gaaatggaag ctaagggggg ctacgtcggc tttgggcttt gaggagaagc gactcgactg
     7321 cgattcaagc agtgccacaa tgtggtgatc cagtcatcaa tgagaatggt gagattcgca
     7381 tttctctcga tatcctcgca ggcctggaga cacgtctcca cacaatgttg gatgacttcc
     7441 accgcatcgg ggccaagtat ctctttgata acgcgaacga agttctggca ttctgctgaa
     7501 gtggagtcgt ctgcgtgccc ccctaccaag agtgttatcg catcgcgcaa gaaattagcg
     7561 gtaaaagaaa ggatatgata aagccgcttg cgacgggcag cggcctcctt gcccgcgtta
     7621 tccacgaacg tgcgaaactc ttgggaccag gtaacggcgt cgaatgaaat accgctcaac
     7681 tccgcccgcc atcttaccca aaacggccac atttccgggt ccgccagatc tcgtgcccgt
     7741 tcaaggctac cgccgctaag ttgggcaagc ctgcgagcct cgtctaaatt ctgcgacagt
     7801 ccctcctcca aaatcagctc ggttaagata tccactggta atggtcggaa gcgcacaatc
     7861 tgacttcttg accggattgt cggcaactgt ttgctcgctg acgtgccaat gagaatcaac
     7921 aatgcctgag ggggcggttc ctccagcgtc ttgagcaagg cgtttgctcc ttccgcgttg
     7981 aagtaatcag catcgtcaat aatgccgatg cggaatcgcc ctgagtaagg tttgagcgcc
     8041 aaatgaccaa tcaaaccggc cccctccacg ttccgctgcc cgcgctcccc gatcaaggtc
     8101 tccaagggaa tgaacgactt ttccggcggt cgcgaaacga acatgacgtc aggatgcgtc
     8161 cttgaaatga cttggagaca cgaggcacaa gcctcacagg gatcgaacag ctcggcaggg
     8221 tgtttctcac aaagcagggt ttgggcaaat ttcagggcaa acagcttttt cccgattccc
     8281 ggcggtccca aaaaaagata cgtccccccg attcgcccac ggctcaccgc ccggcgaaat
     8341 ctctcgacgt tctcatcgtg tcccttaatc ccgtgccaca tcgcattcca cggatcgcta
     8401 acggaaaacc atcattaacg tgtcaaaaac tgacggcctc atttgaccat ctctcgccaa
     8461 atatgttttc aacatcccac gtagccttat cgccgaacgc agtaaccgat tattcaccgt
     8521 gcgatcgcct ctcgcctaat atccctgcct tacttaggct gccccaaaaa cggctccacg
     8581 atgttcctga tcgaggcctc cacttcatcc ggatgccggt cggcccggac tacgatgacg
     8641 cgatcggcgt agcgagttgc ctcctgcaga aacccgtcac gaacccgctc gagatactcc
     8701 acgccacggg actccaggcg gtccggcgtt ctttggattc tttgtagggc ttgggcgacg
     8761 ggcatatcca gaacaattgt aacatcgggc tgaacaccag ccgtcgcaaa gtccccaatt
     8821 tggcggagcg tctcaacgga caatccaaaa ccgtatcctt gataggcaat attcgaaagc
     8881 acaaagcgat cggaaaccac aatgcatccg gcctctacgg caggacggat tacttcggcc
     8941 acaagctgcg cgcgcgaggc catgtagaga agtgcctccg cccttgcatc aaggggcacc
     9001 gcttggcgat gcaagaggat atccctaacg gcctcgccaa gggctgttcc ccctggatct
     9061 cggcagcgaa caactttgtg tcccttttcc tccaaccaac gacacaatcg ttccacctgc
     9121 gtcgtcttgc cggtaccgtc cgcaccttcg attgcaataa acatcgtcgc gttataaccc
     9181 tgtgaagcct caactgcgat tgcttgcaaa aggtaaacct ttatcggttt tagcagagac
     9241 tagccgcgaa gagcagcccc ggggtgaacc accgtcagcc gagccgaacc gtttacgtta
     9301 agaatctatc ccaaaaagtc cccctctccc tctgggagag ggttggggtg agggcaagcg
     9361 atcgtcggag tccatcgaga aaaccggcag caattcgagt caccctcacc cccgacccct
     9421 ctcccgccga acgggcgagg ggagattatg ggataggctc taagtgaggg ccggcacacg
     9481 ccgcgggcag acccaattgt tccaaaaagg ctagcgacgg gcacgccaac ccgagttgtc
     9541 cgtggtctgg cggctcacgt tggtgtgcgt cgcttgcact ggcgaagagg gagcgtctcc
     9601 cgtaccttgc tcgctgggtt gattccttga tggtgacggt gctgagggag tggaagaagt
     9661 tcgggaggta taggagacgt gcgaggccaa ggccacaagc tggtcttgtt tccggcttat
     9721 cgccgccgaa cttccgtcgt cagtgctggc gcgcctttgt tgggggacgc tcgtcgtcca
     9781 atcctcccgc cttgtttgtc gcgacgcatc tttctgttct gcagcttgtt cagttttaga
     9841 agcatcgctt gttgaacccg gcatgtcttc gctgggcatg ggtgggggtt caagctcgat
     9901 cacagaaggc gcaccctgct gatcgatttc cacgccatcc ggtaaaccgt cgatcaggag
     9961 ctcctcagcc ccatccttcc cagcatgaag atcatacggc ccacggctga agaccggtgg
    10021 attcgtcatt ccgtagttca agtagagccc ggcatctctt gctcgtgccc gccgcaaggc
    10081 atcgaaatag gccttttgtg gccacggtcc ttctgtgagg taaacgccgt tgtactctaa
    10141 cagggacccc ttgcggaaat gcaaatggct gatggcaaac atgtatttcg tcaatgactg
    10201 gtagtactga atttcggctt ccgcccgacg ctgttgggca ttcagtaaca agtccaatgt
    10261 tgccgtgccg gcatcgtaca tggcagtgac agcgcggacc tcgttttctg cagccaccgt
    10321 tcggttgtag ttggtctggg caagggtgta atactcgtcg atctcacgca acacccaggc
    10381 aatctggtga gatatctcca attcctgctc ttggaggata gcgcgttcac gtgccaaatt
    10441 gagctgggca ttccgcaccg cagccaattc ttttcggaac ccaatgggaa tctgcagttc
    10501 cattcccaat tgccaatcct gatagtcccc cgatgttagc gacccatacg cgttggagcg
    10561 atcatcatcc ggatcgatca aatcgtgccc taggccgttc catctgtaga acccaaacat
    10621 atcaagtttt ggcaggagat aattcttcgc agcaatcaac tccaattccg cctttttgac
    10681 gcgccacttt tctcgccgca agagcggatt gcggatgagt gcctcagcgt gaacgtcgtg
    10741 aaagtcaaac tccactcttg cccgcgtagg ttcgtccttg ggccgaatga gacgcccatc
    10801 cgtcgctgtc agccccatca aataccgcaa gttattttcc gccttgtata gtgaacttag
    10861 ggcttgctcc acggctgcgc ggaaggccag gtattgttga cgcgactggg cctcctcctg
    10921 ggcacttccg ccgcgtccac ctacctcgta tttggcccga acctgacgcc acgtctgcaa
    10981 ggccgcatcg cgtcccgcaa tcacggtatc caattggcga tagctgagat acaacgtcca
    11041 gtaggccttt tccacgtcga ggagcaactc cgagacagat tgctcgaact cggcaagcct
    11101 ctggtcggtt tctaaccgcg caatgaggac gccattataa aaacctggaa tactgttggg
    11161 accggcaatc cggttgaaag tcacccccgc cccctgaagc aacggttgcc gaaaaccggc
    11221 ctccacattc gccgtccaat ggcttgacca ctggcggctc gtgaaggcat tattccagtc
    11281 gtagcccaca gaatgcgaaa tggaaaacgt cccacccgtt gcgtacgtct tcgagatttc
    11341 ggtctcgaaa ttcccggtat cctgacggaa ggcttggggg cggaacgctt ggacaaatcc
    11401 ggccacgttt tggggtgtat cagatttctg ccagaaaaac cgagtgctcc aatgggcatc
    11461 gaagtcggcg agaacacctt ccacccctgt tcgcgggttt gtttcctggc gtgccgggtc
    11521 atagaccgtg ggcgtccctt cgggcgcgac ggtcaatgat gtctccggca atcgcggcat
    11581 tcccacgtcc ggttggctca aagtgcgaat gatcttgcta ttctgcaggg cgatccgaat
    11641 ggcctcttcc agggataagt cccaatactc tttcacatcg ctgttggata gcgtcaaggg
    11701 cggtgaagcc ttttgtactt catccagcga ctctgcctct acatcggggt actcgatctg
    11761 tgtggccttg tccacataat gggagagatc gccatcttcg tgcaagaaaa acggttgttg
    11821 aggacggcac ccacagagat aaagcgttat aagtgcccaa gccgtgattc caaggaggcc
    11881 gcgagaggtc ttcattggca ccgatccttg tttcgccgaa cgcagcgcaa cagtcctttg
    11941 tttggatcct ccccggcaaa acgccgagcc aggtttataa aatccgcgga attggtatcg
    12001 gcgacctgat cgttaaactt tgacaaatcg gaatatcttt ccagtcaaac cttcaataaa
    12061 ccacccatat ttcgcagagc acctacatta acactgtgag gcggtggaga agctgatggc
    12121 tttttggtca gttggaaatg ggaagccact atagcgtctg gtttcggacc actcattcca
    12181 gcggccgagc ggcgtcggcg gaagaaggca ttctcggcct cccatgacct tgggcgcagt
    12241 aagtgggaaa gtatgtagaa gatgtgtagc aaaactacgt cggctctttc tcaccgtaga
    12301 gggtgaggat gaggtgtcgt ccggaattgt tacggcgatg ttcgcacaaa aaaatcccct
    12361 gccaagtccc caaggccaat cgcccgttgc gaaccggaac gatcaacgag caatcagaaa
    12421 gtgtcgtttt gacgtgagcc ggcatatcat cccgcccttc tactgtgtgg cggtacggga
    12481 gatcctccgg ggccaatctg tcaaacacct gatggaggtc gtcagccacg tcggggtctg
    12541 ccgcctcgtt gatcgtcaat gacgcagagg tgtgctgaat gaaaatatgg aggagtccca
    12601 cacgaatcga cttgagctca ggcaacgctt ctacaatatg tcgggtaatc gcatgaaatc
    12661 ccttgccaaa tcgcggtagc tggagctctt tttgaatcca aaccataggc ttactttctc
    12721 aagttacgta acttcgcttt tttcacgtgg cgataagctg aaaaaaccat tctgtaagac
    12781 tttaatttgt catttttcgc agtcagacag caacatgcgc aaggcttcgc aaacggccct
    12841 ggcatcacca atcagcacca gtgggtcagt ataaaacacg aagtgacctg gtggatatgc
    12901 ccgaggggac aacatgccgt agattgtacg gggaattgac tcacctgtgg ctcacaccag
    12961 gacagatgaa ttctggtctt tagcacagta tcctttggag gatacgattt gccaaccggt
    13021 tcggtataat ttcgtgaaac gccgtggccc cctgtaatcg gagttttgca atgtcctgca
    13081 agcatcttca ggaactttat cagctttgtc accaatacaa cctcaagctg acaggatccg
    13141 agcttgtgcg gatcgtctgc ccgacgtgtg gcgtcgatga aatttgtccc aatgtcttga
    13201 tggaggaata tgatcgtcgt cattccccgg aagaggctcc cccaacatcg gccgatgaga
    13261 cgaaagtgac ttctcctgat caagtaaagg gttcgtctga atagggtttg gcttcgtgcc
    13321 gcgtccagtc gcacgcggct gtcaaatcaa catctcactt tcggacaaac gttcgtttgt
    13381 tggccgacga aaaaacaccg ggcctattcc atcgggactg ctgaagcgat agattaaagg
    13441 ttttcaattc tagggtctcg accctttgcc aaccggatag cgtattaatt gggaggataa
    13501 ccgcgttgac gggcttattt cctttccgtc aacgattgga tacgaaacga aacggtagat
    13561 ctgggtacga ttcatttgga ggactctgat gacagcaatt gtaaaccagc aaacgtgcaa
    13621 cgggtgcgga gagtgtgtcc aggcctgtcc gttggatgcc atcaagattg agaacgaaaa
    13681 ggcgttcgtt gatcccgatg tctgcgggga ctgcggcgct tgtgtcgatg tgtgccccac
    13741 acagtcgatc tcgctttcct aattgaattg gctccttggc gagaaagacg gcgaaacaaa
    13801 aaccgcgacc gaaaatcggt cgcggttttt aattctcacg gtcaaccatt tagagagccc
    13861 cgcctacctt acgcaaccgc gacgcgcgct ggagcggaag tcatcaacac actactgctt
    13921 gatgcgcggg cggcttcttt tgccctaaaa ccttccatcg cttcgtgcca aattacccac
    13981 attaaaagcc tatcccaaag ttccctctcc cccagggaga gggttagggt gagggcaagc
    14041 aacggccgga atcgatcgac aaaaccagca gcgattcgac tcaccctctt cccaggcctc
    14101 tctcccggcg aacgggagag gagagattat gggatgggct ctatgggctt tctccatcgc
    14161 ggggcaattc tcggatgaaa atattcttga agtaaagcgt attcccgtgg ttctgcaatt
    14221 cgatcggtcc ggtgggatag atgggcttgt cgcgttccca gtagttctcc ataaccacat
    14281 tgtccacaac caactcgtcg tttagccaaa cgctgacccg ttcgccgacc attcgaatac
    14341 ggaagcgatt ccattctccc accggtttgt ccgcacactt aagcggtttg ctgggatgaa
    14401 ttttgttatt gtacagtccg ccggacccct cgggatgatc cgtcgcatcc cagatttgta
    14461 cctgggggga gccgcgtaga tagataccgc tgtcgccgtg gggaagaatc ttccaatcca
    14521 cgagcaactc aaagtcaccg tagtccttga ccgtacagag gttgtccccc ttcccgtcaa
    14581 agaccaagac accattttcg accttccaat gctcacgcat ccgctgatcc gccacttcct
    14641 gggcccgctt cagttcctcc ggtgtcattt ttgcccgggt cacggggttt cccactagcc
    14701 ccttccaacc cgtcaaatct tttccgttaa agagggccac aaaacatggc gggggcacat
    14761 tgtcttcacc tggggtctgt gcacagacaa tcgataacgg caaaacgagc aaaaatggcg
    14821 tcacgacatc gagacatctc atcgaacatc ctcctgttac gaagtcaata tcgctcccac
    14881 gacgacgatt gaaatgttac acagttttgc atcccaaaca aaccaagtgt gtcacggagt
    14941 ttttggtttg ggacggtccc cgtagtacgg tgctcgatcc caaaagtcag tgtcgcccga
    15001 agccctagga tcgcctgtat cacgcatcca agcttgaagt ttttccttta gttctttctc
    15061 gatggccgcc acctccggct tcccggccaa gttattcatt tcgccaggat cagccaacag
    15121 atcgaacagc tcgaattccg gccttttggc cccacccgct gccaagaaaa acggcctaat
    15181 agccggatcg ttttgccgct ggagcatctc cgtccacgtg ggagaaccat caatgtctcc
    15241 ataatcccgc cccaccgact cctccggcgg attaccggca ggccaaaggt gcggtcggag
    15301 gttccaaatc aggtgccacc tctctgtgac aatggagcgg atcggatagc tcgcattgtt
    15361 gggccgcgta caggtgtgtc gctctcgctc ggtaaacaca tgatcgcggc tcgggtcaac
    15421 ccgacctgac ttgtgggaaa gcaagacgtt gaggaaactc ttgcctgtca tttcgggcag
    15481 gggcttcagc cccgcggctt ccaagattgt tggtgcgaca tcagtcaagc tgatgaagtc
    15541 atccaccact cgccccccgg ggacctttgc cttccaacag accgccaatg gaacgtgcgt
    15601 tcccgcgtcg tagcacgtcg cctttgcttt cgggaatggc atgccgttgt cgcccgtaac
    15661 gatgattaac gtgttgtcca gtcttcccgt cgcttccaga agtttaatca cttcgcccaa
    15721 ctcgcggtca aagcgttgga cctcaaagta atagtcggca atgtcgcttc gtacaacagg
    15781 ggtatcaggc aaaaacggcg gcacacgaat cttgttcagg tccatgcccg aagcaacacc
    15841 actgcctttt tcataaggtc gatgaggatc agtggacccg aaccaaaagc agaacgggcg
    15901 accgtctggc acggttttta aaaactcctc gaaattgcga aactgagggc cagcaggatt
    15961 acgttgcctt ccggtgccct caaggacacc cggtccccat gccttgcgca tgatgccgat
    16021 cgcatagccc gcttcctcca aaatatccgg atagcagcgg aacttggccg gcaagcggct
    16081 gtgaaggttg gcaccttcct cgagtcgatg ggcggcctga ccggtgagaa tccccccgcg
    16141 cgaggccgtg caggttggcg caatgacgtg tgcccgtgta aaaagcacac cctcgcgggc
    16201 aatgcggtcg aaggtgggcg tttgcaccac tggatcacca taagcacccg catgaggcca
    16261 accccaatca tcggcaatgg caaataagat gttcggcctt tcctccgttt ccccacccca
    16321 tatctggctt gagactctca gccccaggct cacgagcaaa atgcccggca aaaccctacg
    16381 aaagaaccgc attgacggtg accgattcat ttttcagcct cccacctatt ggttagccac
    16441 gccaagcgat taacctaagc gaatatggcc agttccaaat ccgagcgtaa catttctctg
    16501 cgatgaacta ttttgcccat gctgtgcgat ttctcgatcg tccgtacttc ctggcgggca
    16561 cctcccttcc cgactggttg tccgtcgtga aacgcacggt acgactgcgg cgaacctcgt
    16621 taaccgagca cctcacctca ccggatgagc gcacccgaca actcgtcgcc ggtgttgtgc
    16681 aacatctcga agacgactat cgtttccata gtgcgccggc gttttcggaa gtcctggttc
    16741 aggttttacg actaatccaa cccgttgtca acacgccggg cgtgtccttn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tatccggtcg atcgtggcgg cgaaggagct
    16921 cgatctttgg cgggcgctag acggctaagc gtgaacttgg cagaataggg tgacggcttt
    16981 tgccgcgttc gtttcgcggc gagccgcttg gccttggtat gggcagcctt ctgggagaag
    17041 tatgcatttg acaagcggag gtgaatcatg cggtttggac tcaatctttt gctgtggact
    17101 gatactctgt gcgacgaata cctccccctt ttggacgaac tgaaggaaat tggcttcgac
    17161 ctcgtggaat tgccggtctt tgaccttgac aacgatgcca actacaagaa atgggctaaa
    17221 cggctggatg aacttggatt ggcccgcacg gcgacggcca ttcgcacggc agaggagaac
    17281 cttattagca gtgacccggc cgttcgtcgt aaagggattg aggccaacaa gcggaaccta
    17341 gactgttgcg ccatgcttgg agccgagatc atggccggcc cgttccactc ggctttgggc
    17401 gttttttccg gacggggacc caccgaagac gaatggaagt gggccgttga aggaatgaga
    17461 gaggtggccg agtacgccga caaggtaggc gtcaccttgg ccgtggagta cctcaaccgc
    17521 ttcgagtgct acttcctcaa cactgccgct gacggcgcga ggttttgccg tgatgtcaac
    17581 catccgcgct gtaagatgat gtacgatacg ttccattccc acatcgaaga aaagaatatc
    17641 ccccaggcca tccgagcact gaaggattac cttgtccatg tccatatctc ggaaaatgac
    17701 cgtagcaccc ccgggaccgg caatgtccgc tggccggaaa ccttcgacac cttgcacgag
    17761 attggataca acaacacgat ggtcattgaa gcctttggcc tggccctcga taaactcgtt
    17821 cccgctacga agatttggcg aaggatgtat gaatcggaac gccaactggc caccgatggg
    17881 cttcgattca tgaaaaccga agtcgccaaa cgctggaaat agtcgtaaag cctttcccat
    17941 aatctcccct tacgcattca gcgggagagg gggaggagac gggcgtgtct cgaatcagtg
    18001 ccggttttct caaccgcttc cgacggttgc ctgccctcac cccaaccctc tcccggaggg
    18061 agagggggcc gttttggcat aggctcctag gtgggatttt ggcgaagatg ctcattggaa
    18121 gagagtgaag ccacgggcct gccttctccc tggtccgcca atcggggttt ttcccggcgc
    18181 gcggatgtgg atagaataaa agggacgaag ggcaagtctg tttttgcgca ctacgggcct
    18241 tgttcctttt caagaagaga ggagttgtgt gatgtttgcc cgaaagcccc ggggttttac
    18301 ccttgtcgaa ttgctcgtcg tgattgtgat catctcagtc ctggtggcct tgcttcttcc
    18361 tgccatcgtc ggctcacgag aatccgcccg gcgtgcccag tgtcagcatc aactatcgca
    18421 actgggccag gcgttgcaaa cgta
//
LOCUS       DAOSUT010000078        18101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1957_length_18101_cov_9.629170, whole genome shotgun sequence.
ACCESSION   DAOSUT010000078 DAOSUT010000000
VERSION     DAOSUT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_18101_cov_9.629170"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..3652
                     /locus_tag="PLD05_08815"
     CDS             134..3652
                     /locus_tag="PLD05_08815"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP77581.1"
                     /translation="MDRFSGVELSRLWIHLLCALAGVCGLMQFGLGKEPPTVQSSFAP
                     QELVQGFRHPPSSARPAAYWCWMNGYVDEPQALWEIQEFRRKGLTCLYVFDIGARDGD
                     VKVPAGPAFMGPQWVKTFANVVREAAKCDMEIGLITSSSWNAGGPWVTPEYGAMGLFT
                     TQLTFEGPADFTATLPQPELPAAASKHPAGLPPIYRDIAVLAVPAENVYDRPTFVFQL
                     APPGRHVVDHVILCNTESEDPQVTGPLHRFVKEFAVYLSDTNDAPDAFRLVLKGELQP
                     TVEPQRFDFPATPARFVKLELLSRHNPAQTRWELGEFELYSKEGKNVVSRMVENGSRT
                     GAALIRFTTQWRSSGPYSADCIHDGVRNGPKGSWASADQPPLLIADPGQVVDLTAHLD
                     DQGSLHWKVPPGKWRIIRFGYGNTGQSLVLPSPNSAGWVIDHFNPAATDWHFKHILEP
                     LIEELGPLNRTALKQLYVCSYELRGATWTPALPEEFARRRGYDLKPFLPVLVGCIMKD
                     ETTTRRFIRDYETTLSDLIVDGFYGRATEICHQHGLKLCAEAGGPGLPLHPVPVDALK
                     AQGAIDIPRGEFWVDEHIWVVKETACAAQIYGKPIVDMEAFTSWRHWQDGPAELKPIA
                     DRAFCEGANHFTFHTAAHRPKEAGLPGWVYSAGTHFSPSIAWWPLADAFVDYIARCSF
                     LLQQGEPVTDVCYYYGDRGFNFVMEKSIDPELGPGYDYTVTSSEALLTRAAVKNGRIV
                     FAPDQEGVALLVLPPGSELTPEVAEKLESLIREGAIVVGTKPQRSPSLTGFPSCDEKV
                     KAIGDRIWGENPPPKGERSYGKGKVFWGQSLREVLQRLEIPPAVVCDAATDASVDFVH
                     RRTASAEIYFFWNRLPRWEHLLVRLRAKEKLPEIWNPVSGQMTPALAYRKTQQGLEMP
                     LDLPPRGSLFVVLSDTTAAQSAGPGLDPVVSVTRDGRELVFAPASGEAPVSVQPNGQG
                     LPLLSAAESGTYKLRFQSGRELAISVEAPQVLQVPGPWQVEFPPGWGAPERITLERLI
                     SWTDHPNPGVKYFSGVATYRTTFRYEPVSEPRRVVLNLGEIRFVGEIALNGKVLGILW
                     TPPYELDVTGVIKEGENELVVRVANVWSNRLTGDAREPQFGTFTRTNIRNALAWRVPW
                     KDAPLHPSGLLGPVTVKTVKLPTQTP"
     gene            3698..7684
                     /locus_tag="PLD05_08820"
     CDS             3698..7684
                     /locus_tag="PLD05_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77582.1"
                     /translation="MIFRKSDFPVCRVMPSFGRTLLSVVLFSLIRHGGNLPFLERPIF
                     AESPPTGIDNPCTNGSFEVLGPNGFPVDWGPVGREVSVSEDAHTGQRSLRLVRSADTP
                     TPETGLNRTKLISELKGGVDFWYKAVSAKGAKLNVYVIPVNDQGIERTGSPRATFTVP
                     EDHIGDGQWHHARLKYDYSDNPKVKSVHFAARIVGTSGEILLDDIQYVAEVGPVPHIK
                     KVMLEENSEAPGQRGTIRVLVTNLGDEPAKDVKVTVTLPGKTTFRPVEMQLPILRPDE
                     IKPLYCPLAVVRNEPLTVHAKVVAGELEDTRRLELTPALRVRSFGPTMPVVTVGQTVV
                     LECELENIGAITLSGVLVTFETPRGASRQVVKSLAPTRRTVVRHTFSVDAETMQLPVR
                     VIVQSQGIAIAEGSDGQWTAESRIVVVKAISPPTPVGRFSVVPRPIPILENEHFRLVS
                     YPCEQRFFAMRLEINTREGWKVAGWLVSMGRLVLRTARGQVQEHLLAADSCELAPPPA
                     KREAVATFHCPLKTSVGNLTARVTFRIAPNIKSCGISADLQADQPVDILAWGLPMIYL
                     HERDEAIFPGLEWLVKDELSSDTLDIAEDHLDRIRYVVHPNMITIPAVGIHASWGTVG
                     LMWYLQPYGQLLQPAFAFASPDRFNHQRSHLMELFLPPVPKYTPVNSRVAEKPYRLEP
                     GKSLILRANLWMDGEASDALAVVEEYVKRHKIAPAHPLPHTSLPGEIIFSARAYLESL
                     WDAETRKWWTTKGNAILSHQALPPDFAADLALARMLTGDQELKKRCEERLSEVCQVLG
                     LPPRLDALRIGGRADRVWANAGRVAALLASRKEDGSWRFDADRPGSGPFVGMDYRELG
                     PHDALEVGTCARNAYEVLAFARITGDHGLYQEMVKTLELMERFRVPRAAQVWEVPVHT
                     PDILAAADAVDAYLEAYQLSGEKRWLEDAVLWAKRGLPFVYLWNDPEKPYLQGASIPV
                     FGATWYRGSWFGRPVQWNGLRYANALLKLAQYDQSLPWKEVATILIHSAVHQQDLEGE
                     NVALWPDNISAITGEKCPWVFAPRQIIGCINRLLGREEEPQTIYLKLGDRSVPLSSLG
                     KIGRPQVEGNALTFTVQFPPGEVGPVLIANVAAPQEVLINGQPVPQNERPHLVEGSSW
                     CYDSGMALLTIQVGLSGPAEVTVKPLNVRTVDRIPRIVNVIRFYFDEGMEGWMAAHDV
                     ADLQVKAGQLVGRIVGPDPYIVRPHMRVPGAKGDTIIIRISTTAGSMAQLFWATESSP
                     SFDEEKSLRFQVQPGETVNEYRLPLGDHPSWNGQTVVGLRLDPGGGTTEGSFVVDAIE
                     GVTASGQR"
     gene            8062..9450
                     /locus_tag="PLD05_08825"
     CDS             8062..9450
                     /locus_tag="PLD05_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HOP77583.1"
                     /translation="MHRSAYRCLVVASLALGFGFLSWGTSAWAQQQDDYQMAGVIVSP
                     DGVLTRQMISDPTGQIMRERVMAARRSLPPNVARFSTLRKVSLPRLEKAIVANHGVVT
                     DEMKYLAGLLRIRYVFLYPESGDIVLAGPAEGWVETPTGRVVGINTGRPVLQLQDLVT
                     ALRAYPPDGEGVDVIGCSIDPTQEGLARMQEFLRSLGSTFPPGQEAQVAEYMVNGLKK
                     SLGVQEVTILGVPPETHFAHVLVEADYRMKLIGIGLERPPVRLVSFVERAASSAVSPN
                     QLFRWYFVPDYQCLRVTEDGTALELVGEGVKLVGEDELVTASGERKAASAASAASRAF
                     TTSFTRHYPELAAKVPVYAELRNVIDMAIVAAYIKQQDFYNQANWKMEFLGREENFAV
                     KTYPAPKKAETAITAVIRPGRVSTPIGGGVHIEAQQALDYENLLKDEKGEVQKAKASV
                     KVDLPEGVWWWD"
     gene            complement(9460..>10221)
                     /locus_tag="PLD05_08830"
     CDS             complement(9460..>10221)
                     /locus_tag="PLD05_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HOP77584.1"
                     /translation="LETALEHYHEWRNHPDENGVSGLAPYLHFGFISPFEMIVDLLKR
                     ATEMGWSFDRPSQEADAGTWWNLPPSIAQFLDQLVVWRELGLNLCLRCDDYDRFESLP
                     DWAKKTLTAHASDRRPITYTLEQLENAQTHDRLWNAAQNELREEGRLHNYLRMLWGKK
                     ILEWTPDPQTALEWMIYLNNKYGLDGSDPNSYTGISWIFGRYDRPWGPDRPIFGLVRY
                     MSTDSARRKIRLKKYLERYAPEMKETQVTTEDVKD"
     assembly_gap    10222..10321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10322..10916)
                     /locus_tag="PLD05_08835"
     CDS             complement(<10322..10916)
                     /locus_tag="PLD05_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77585.1"
                     /translation="MRGNRLIASPATAFAEAASQILSGKIWGGKVPEERIRVIAEGTP
                     HQSPNVVLYWMHINRRWSWNYAVDRALEWVADREKDLTVLETLPFHQTLTDRHAVFVM
                     EGMREHESLVERGPFLYRAQSRKFGRIAVEYLYSLFQTCDVIIVDDHPDREYRAFLES
                     LASRTRARIEAVDSSGLIPFRSVSRYYPTARGFRTLVQ"
     gene            complement(11002..12486)
                     /locus_tag="PLD05_08840"
     CDS             complement(11002..12486)
                     /locus_tag="PLD05_08840"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77586.1"
                     /translation="MARQVTPRREFLQEAILASLGGIGLGAGLPVVAAEQGPQSAGAA
                     APTSPNDKLQVAVLGVRGRGQDHASAYASRPDCHVLYLCDADRDVGQTYVERFAARFG
                     YKPKFAQDLRDVFADPAVDLVSIATPNHWHALASIWAIQAGKHVYVEKPVSHNVSEGR
                     RIVQAAQKYNKLCQGGTQRRSEGRYRAAAEAIKAGKIGKVKVIRCVMYRPRSPIGPPG
                     DYPIPPGVDYNLWAGPAPMAKVTRKSFHYDWHWFWDYGNGEIGNNCIHCVDTARIIVP
                     FKTLGKWVISYGGRLGFNDAGETPNVQVAVHDLGGITLVQEVRNHKTNPPFLNGAVLI
                     VGEEGYIASSFGPNVVVYDKQGQPRENLTGPNVDNFGNFVEAVKAGKQEMLNAPVEET
                     HISTALTHLANISYLLGEKAGHKELVAALEKLQVHEDCVATLNAIEEHLKENGIDLEK
                     EPLTLGALLPVDAANECFIDRPDANKLLTREYREPFVVPTVDKL"
     gene            complement(12624..12965)
                     /locus_tag="PLD05_08845"
     CDS             complement(12624..12965)
                     /locus_tag="PLD05_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOP77587.1"
                     /translation="MENTKALYRPLLGQAQGARKLHVHLTELNGADGWNGQHAHAAEE
                     AVFILEGEAEFTFGGRTYRVGPGEMVFFPPNVMHAEARFFSDKMKYLVIRTVEPGDEP
                     CCCETEMPSEQ"
     gene            13161..14138
                     /locus_tag="PLD05_08850"
     CDS             13161..14138
                     /locus_tag="PLD05_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOP77588.1"
                     /translation="MTVYDVLFQVWWFVLGAVLGSFLNVVIHRLPRKESLIWPGSHCP
                     SCGHLIRWYDNVPVLSWLFLRGRCRDCSATISPRYPLVEAMAALTVGGLAYAVSGGRW
                     GALLSTGQGTWLAALFAPAILHLLIYATFFLTLMAAAAIAWDRAPCPPLLWLPAWVLA
                     LIGVVVGSKGHAGTAFRHLTLPMFSWFHWGLALVLGSINELLLSGWESNPGGNGPSSQ
                     KRSRKERRKKRQRAEVNPRDHEGQAEIERSRSVDFISFAGLAGILFAGESAVALVGGL
                     LLLVVFIRAASLLSSSRQRPAGDPSRVRAVLLGIAFVASWSAILLEMTR"
     gene            14216..16666
                     /locus_tag="PLD05_08855"
     CDS             14216..16666
                     /locus_tag="PLD05_08855"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77589.1"
                     /translation="MQTRLLRFRRAFCAPWGIMLVFLLTVAGGKGASNVGLADTPSHG
                     VDPRLVAMVESDWQKQETRLGRDCASVEAIKAALRRLEAALEELEKARLIPEERLRDA
                     PTTLKGFWERTALASTLPAEERTKLYREIRWFGRSLLLENRLFADRPIVFLKTRRFLC
                     QMLHEYIAYFYSAADLHGGGVYVLKKPGESLEIEDLTTGHFGRGIFQSLALSYDAKTV
                     YFAFAPIPVGDRQRPLRPDWSVLTAGPLPPEFAFESPDRPCFHLFAIDLTNRTIRQLT
                     DGRYDDTMPWPLPDGDLVFMSTRRGGYCRCNNPWEPIQTYTLHRLNPKTGAIRTISYH
                     ETNEWHPAVLNDGRIAYCRWDYVDRSAAHFHGIWVTSPDGTNARSVFGNYTMEISTCF
                     QPRPVPGSRKIAFIAGAHHAVVGGSLVLFDPDKARYDPETGEDVFDSLEVLTPEIPFP
                     ETPSEWPTGYVVSPWPLSEDFYIISFGHDRLPGMGSGNRSESGVGIYYFDRWGNLELL
                     YEDPGWACLDPVPVIPRRPPPVVQETFDPDLGQEAEVVVYDVRQSHYPLPPDRPIREL
                     RVFQLLPKATHPVNNPRIGHANAENARALLGTVPVEEDGSAYFRVPADRPLYFQIVDD
                     RGRAVQGMRSVTYFRPGERRGCIGCHEPPNSSVPTRDILALRREPSALTPGPDGSLPF
                     SFPRLVQPVLDQFCIRCHSPNAGPQFAAPDLMGLPQGEFTRSYVNLRPYLRWYEWGGG
                     SISQIVTRPGQLGADASPLLNILENPTHREAVTLDRESLLRLTLWLDANVPFYGTYDN
                     DQRAQQHAGQLIAMPELQ"
     gene            16888..17784
                     /locus_tag="PLD05_08860"
     CDS             16888..17784
                     /locus_tag="PLD05_08860"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOP77590.1"
                     /translation="MTPEERQAAVERILRSPSYRVAYQDLDFLTDPKLRATRMELELL
                     KPELAFEREKINSTVVVFGSTRIVDEPEARQLVEKAEADLKADPENPKLRRAYERARS
                     LLEKSRYYELARRFAYLVSQSCANNGQCDYVICTGGGPGIMEAANRGAYEAGAKSIGL
                     NIRLPMEQIPNPYITPELCFQFRYFALRKFHFLLRAKALVVFPGGFGTLDELTDALTL
                     RQTGRMQPIPIIIFGREYWDRVLNFQALADEGVIDDEDLKLFEFVETAEEAWEKICRF
                     HHLSMLEAKPVYWENSGGIDPH"
BASE COUNT         3659 a         5078 c         5236 g         4028 t
ORIGIN      
        1 gaggactcac gatcgggcaa cttgacaatg cgctcgtcct cgctcacatt tttggcaggg
       61 ttactaggcc catggtcgtt ccaaagattc accgttaggc cgtgttcact gggaaagagc
      121 tatgagacgt tgtatggata ggttttccgg tgtggagctt tcgcgattgt ggattcatct
      181 tctgtgtgcg ctcgcggggg tatgcgggtt gatgcaattc gggctgggga aggagccacc
      241 aacggttcag agcagcttcg ccccgcaaga actggttcaa ggcttcaggc atccaccgag
      301 ttcggcacgt cccgctgcct actggtgttg gatgaatggc tacgtggacg aaccccaagc
      361 cctctgggaa atccaggagt tccgccggaa aggcctgacc tgtctttatg tcttcgacat
      421 cggtgcgcga gacggcgatg tgaaggtacc cgccggaccc gcgttcatgg gccctcaatg
      481 ggtcaagact tttgccaatg tcgtccgtga agcagcgaaa tgcgacatgg agatcggcct
      541 aattacctcc agcagttgga atgcgggtgg cccctgggtc acgccagaat acggcgccat
      601 gggattattc acgacgcaac tcactttcga gggcccggcc gattttaccg ccaccttgcc
      661 acagccggag cttcccgcag cggcctccaa acacccagcc ggtttgcctc ccatctaccg
      721 ggatattgct gtgctcgccg ttcccgcgga gaacgtctat gatcggccga cattcgtctt
      781 ccaactggcg ccccctggcc gacacgtggt ggatcacgtc atcttgtgca acaccgagtc
      841 cgaagatccc caggtcacgg ggcctttaca tcgttttgtc aaagagtttg cggtctatct
      901 ttccgacacc aacgatgccc ccgatgcgtt ccgtcttgtt ctcaaaggag aactccaacc
      961 cacggtggag cctcagcgtt tcgatttccc tgccacaccg gcacggtttg tcaagctgga
     1021 acttctctct cgccacaatc cggcccaaac gcgttgggag cttggcgagt ttgagcttta
     1081 ttcgaaggaa ggcaagaatg tggtctctcg gatggttgaa aatggaagtc gcaccggggc
     1141 ggccctgatc aggtttacca cgcagtggcg ctcctccgga ccgtattcgg cagattgcat
     1201 tcacgatgga gtccgcaacg gtcccaaagg aagttgggcc tctgccgacc aaccgccact
     1261 gttgattgcc gatcccggcc aggttgtgga cctaaccgcc catctggatg accaaggttc
     1321 cctccactgg aaggtaccgc cgggcaaatg gcgaatcatc cgttttggct atggaaatac
     1381 cggccagtcg ttggtgttac ctagtcctaa ctcggccggc tgggtgattg atcacttcaa
     1441 ccccgccgca actgattggc acttcaagca cattttggag cctctcattg aggaattggg
     1501 accactgaat cggacggccc tgaagcagct ttacgtctgt agttacgagt tgagaggtgc
     1561 cacgtggacg cctgccctgc cggaagaatt tgcccgtcgc cgcggatacg atcttaagcc
     1621 tttcttgccg gtcctggtcg gctgcatcat gaaggacgaa accacaactc gccgcttcat
     1681 tcgcgactac gaaacgactt tgtccgatct gattgttgat ggcttctacg ggcgggcaac
     1741 cgagatttgt catcagcacg ggttaaagtt gtgtgccgaa gcgggcggcc cgggattgcc
     1801 gcttcatccc gtcccggtgg atgctctcaa agcccaaggg gcgattgaca tcccgcgtgg
     1861 cgagttctgg gtggatgagc acatttgggt ggtcaaggaa accgcctgtg ccgcccaaat
     1921 ttacgggaaa cccatcgtcg atatggaggc cttcacaagc tggcgacatt ggcaggatgg
     1981 tcccgcggag ctgaagccga ttgccgatcg ggcattttgt gaaggggcta atcatttcac
     2041 tttccacacg gcggcacatc ggccaaaaga ggcgggactt cccggctggg tttatagcgc
     2101 cgggacgcac ttttcgccat ccatcgcgtg gtggccactt gccgacgcct tcgtcgatta
     2161 catcgcccgt tgtagcttcc tccttcagca gggcgagcct gtcacagatg tttgctacta
     2221 ttacggcgat cgtgggttca acttcgttat ggaaaaatcg atcgatcctg aattaggccc
     2281 cgggtacgac tacacggtaa ccagcagtga ggcccttctc acgcgggcgg cggtgaaaaa
     2341 cggccgaatc gtgttcgccc ctgaccaaga aggcgtggcc ctacttgtgc ttccgccggg
     2401 tagcgaactg acccccgagg tggccgagaa gttggaatcg ctcatccgag agggagccat
     2461 tgttgtagga acaaaacctc agcgtagccc ctccctaacc ggctttcctt catgcgatga
     2521 aaaggttaag gccatcggcg atcgcatctg gggtgagaac ccacctccca agggtgaacg
     2581 cagttacggc aaagggaagg tcttttgggg acaatcactg cgagaggtgc tccaacggtt
     2641 ggaaattcca ccagcagttg tttgcgatgc cgccacggac gcctcggtgg atttcgttca
     2701 ccgcaggacg gcctcagcag agatttattt cttctggaat cgtttaccgc gctgggaaca
     2761 cctccttgtg cgactccgtg ctaaggaaaa gttaccggaa atctggaacc ctgtgagcgg
     2821 gcaaatgaca ccggccttgg catatcgcaa aacccaacag gggctcgaga tgccccttga
     2881 tttacctccg cggggaagtc tttttgtcgt cctcagtgac acaacggcag cgcagtccgc
     2941 tgggccgggg ctcgatcccg tggtaagtgt cacgcgcgat gggcgggagt tggtatttgc
     3001 cccggctagc ggcgaagccc ctgtgagtgt ccaacccaat ggccagggcc tgcctcttct
     3061 ctctgccgct gaatcgggga cctacaaact gcgtttccaa tcgggaaggg agctcgccat
     3121 ctcggtggaa gccccccagg ttctccaagt tccgggaccg tggcaggtgg aatttcctcc
     3181 cggctggggt gccccggaac gcataacctt agaacgcctt atctcctgga cggatcaccc
     3241 taatcccggc gtgaagtact tttcgggagt ggctacctac cggactacat ttcgttacga
     3301 gcctgtttcg gagccaaggc gggtggtcct caatttgggc gagatccgct ttgttggaga
     3361 gatcgcgctt aacggcaaag ttctcggcat cctgtggaca cccccttatg aactggatgt
     3421 caccggggtc atcaaagagg gggaaaacga attagtggtg cgggtggcca atgtgtggtc
     3481 taaccgtctg acgggcgatg cccgcgaacc tcaattcggc acattcacac gaaccaacat
     3541 caggaacgca ttggcttggc gtgtcccctg gaaggacgcc ccattacatc cctccgggtt
     3601 actcgggcct gtcacggtaa agacggtgaa acttcccaca caaacgccgt aggaaaaatc
     3661 ggcggattcc aaactccctt aactctggag accaacgatg atctttcgaa aaagcgattt
     3721 tcccgtgtgc cgggtgatgc cgtcgtttgg gcggacgtta ttgagtgtgg tcctctttag
     3781 cctgatccgt cacgggggaa atttgccctt tttggaaaga cctatcttcg cggaaagtcc
     3841 tcccaccggg atcgacaatc cctgcacgaa tggttcgttc gaggtgcttg gcccaaacgg
     3901 atttccggtc gattggggac cggtgggacg cgaggtcagt gtttccgaag atgctcacac
     3961 aggccagcgg tcattacgct tagtacgatc cgctgacaca ccaaccccag agaccggcct
     4021 caatcggacg aagttaatct ccgagctcaa aggcggcgtg gacttctggt ataaggccgt
     4081 ctcggcaaag ggtgctaaac tgaatgttta tgttatccca gtgaacgacc agggtataga
     4141 acgcaccggt tcaccgagag ccacgtttac cgtgccggag gatcacatcg gcgatggcca
     4201 gtggcaccat gctcgcttga aatacgacta ttccgacaat cctaaggtaa aaagcgtcca
     4261 ctttgcagct cggattgtcg gtacgtccgg ggaaatcctc cttgatgaca tccagtatgt
     4321 ggcggaagtc gggcctgttc cccatatcaa aaaggtgatg ctggaagaga attccgaggc
     4381 cccggggcaa cgtggcacca tccgcgtctt ggtgacgaat ctcggtgatg agcccgccaa
     4441 ggatgtgaaa gttaccgtga ctcttccggg caagaccacg tttcggcctg tggaaatgca
     4501 actgcccata ttgcggccgg atgaaatcaa gccactctat tgtccgctgg ccgtggtgcg
     4561 aaacgaaccg ctgacggttc acgccaaggt ggtggccggg gaacttgagg acacccgtcg
     4621 gttagagcta acgcccgctc tccgcgtgag gagcttcggc cccaccatgc ccgtggtgac
     4681 cgttgggcaa acggtggtcc tggagtgcga gctggagaat attggggcca tcactctcag
     4741 tggcgtgctc gttacctttg aaaccccgcg gggcgcaagc cgtcaggtgg ttaaatccct
     4801 tgccccaacg cggcggactg tggtgcggca caccttttcg gtagatgctg agaccatgca
     4861 attgccagtg cgggtcatcg tccaaagtca ggggatagcc atcgccgaag gttccgacgg
     4921 gcagtggacc gccgaatcgc ggatcgttgt cgttaaggcg atctctcccc cgacgcccgt
     4981 gggccgattt tcagtcgtgc ctcggccaat ccccatcttg gagaatgagc acttccggct
     5041 tgtgtcgtat ccctgcgaac aacgattctt tgcgatgcga ctggaaatta acacccgcga
     5101 gggttggaag gtggccggct ggctcgtctc gatgggccgc ctcgtgcttc gcacggcgag
     5161 gggccaggtg caggaacatc tcttggctgc cgactcatgt gaactcgctc ctccgccggc
     5221 caagcgagaa gctgtcgcca cgttccactg tccattaaaa acgagtgtgg gaaacctgac
     5281 ggcccgtgtg acatttcgaa ttgctcccaa cattaaaagt tgcggcatca gcgctgacct
     5341 gcaggccgac cagcccgtgg atatcttggc ttgggggtta cccatgatct atctccacga
     5401 gcgagacgag gccattttcc ccggtttgga gtggcttgtg aaggatgaac tctccagcga
     5461 cacattggac attgcggaag accatcttga ccgaattcgc tatgttgtcc atcccaacat
     5521 gatcaccatt cccgccgtgg gcattcacgc ttcatggggg actgttggat tgatgtggta
     5581 tcttcaaccc tacggacaat tgttgcaacc ggcgtttgcc tttgcctcac ccgatcgttt
     5641 caaccaccag cggagccacc tgatggaact ctttctgccg ccagtgccga aatatacgcc
     5701 tgtcaactct cgggtggcgg aaaaaccata tcgcctggaa ccaggtaagt cgctcatctt
     5761 gcgcgccaat ctttggatgg acggcgaagc gtcagacgcc ctggcagttg ttgaagagta
     5821 cgtcaaaaga cacaagattg cgccggccca tcccctgcca cacacctcgt taccaggcga
     5881 gattatcttt tctgcgcggg cgtacctcga atccctgtgg gacgccgaaa cccgcaagtg
     5941 gtggaccacc aaaggcaacg ccatcttgtc gcaccaggcc ctgccaccgg actttgccgc
     6001 cgatcttgcc ctggcccgca tgcttactgg tgatcaagaa ttgaaaaaac gctgcgagga
     6061 gcggctctcc gaggtctgtc aggtattggg cttgccaccc cgtttggacg ccttgcggat
     6121 tgggggacgt gccgaccgcg tttgggccaa tgcggggaga gttgccgccc tcctggcctc
     6181 acgcaaagaa gacggaagct ggaggtttga cgctgaccga cccggcagtg ggccttttgt
     6241 agggatggat tatcgtgagc tgggtcccca cgatgccctc gaagtgggaa cctgtgcccg
     6301 taatgcctat gaggtcctcg cctttgcccg tatcacaggg gaccatggcc tttatcagga
     6361 gatggtgaaa acactggagt tgatggagcg gttccgtgtg cctcgggccg cacaggtctg
     6421 ggaggtcccc gtccataccc cggatatctt ggccgctgcc gatgccgtgg atgcttatct
     6481 ggaggcctac cagctcagcg gggaaaagcg ttggttagaa gatgcggtgc tttgggcgaa
     6541 acgcggcctg ccgttcgtct atttgtggaa tgatccggaa aaaccctatc tccaaggagc
     6601 cagcatcccg gtgtttgggg ccacatggta ccgcggctca tggtttggac ggcctgtcca
     6661 atggaacggc ttacgatatg ctaacgccct cctgaaactg gcccaatacg atcaaagcct
     6721 tccgtggaag gaggttgcca cgatcctcat ccacagtgcc gtccatcagc aagacctgga
     6781 gggagaaaat gtggcccttt ggccggataa catcagcgcc atcacgggcg agaaatgccc
     6841 ttgggtcttc gccccgcggc agatcatcgg ctgcatcaac aggctgcttg gccgggaaga
     6901 ggagccacaa acgatttacc ttaaactcgg cgatcggtcg gtgcccctaa gttccttggg
     6961 caaaattggc cggccccagg tcgagggcaa cgcactcaca tttactgtgc aatttccacc
     7021 gggcgaggtg ggccctgtgc tcatcgctaa cgtcgccgcc ccgcaagagg tgctgatcaa
     7081 cggccagccg gtcccccaga acgaacggcc tcacttggtg gagggttcct cgtggtgtta
     7141 cgactccggt atggcgttac tgaccattca ggtaggtctc tccggaccgg cggaagttac
     7201 cgtcaaaccc ctaaacgttc gcacagtgga ccgcattcca cggatcgtca acgtcatccg
     7261 gttctatttc gatgagggca tggagggctg gatggccgcc cacgatgtgg ctgacctgca
     7321 ggtgaaagcc ggccaactcg tcgggcggat tgtcgggccg gacccgtaca ttgttcgtcc
     7381 gcacatgcgg gtaccagggg ccaaaggcga cacgatcatc atccgtataa gcacaacggc
     7441 tggtagcatg gcccagctct tttgggcgac ggaaagctca cccagctttg acgaagaaaa
     7501 gtcactccgt tttcaagtcc agccaggtga gaccgtcaac gagtaccggc tgcccctcgg
     7561 ggatcatcca tcatggaacg ggcagacagt ggtgggacta cggctcgacc cagggggtgg
     7621 caccacagaa gggagcttcg tcgttgacgc aatcgagggt gtgacggcat ccggccagcg
     7681 gtgaaacgac aatcgcttgc tatcccgccg aatgtactat tagtccccac ccttgtttgg
     7741 tactcgggga atcattttgt caggaacgac tcgtcatttt ggccgtggca aaggacgtgg
     7801 ccactcggcc acaagcgctt gcccacgcga accgcggaga gaaatctcgt cctaaattat
     7861 ggatttttgt tgtcgggtgg catttgcttg aattacctaa cttgcccaat tatgatcgaa
     7921 ggggtaagtc cgatagtgat gtgacgcctt catgggaact ttttccgtga gcgccaccgt
     7981 ctaaggacgc aacattggcg ggcgcaacat cggccgtttg acaacgatgg cctgtaccaa
     8041 gtggtgatgg agaggaaact catgcatcgt tcagcttatc gctgccttgt ggtcgcaagt
     8101 ctggcgttgg gttttgggtt cttatcgtgg ggaacatctg cttgggctca gcagcaagat
     8161 gactaccaaa tggccggggt gatcgtcagc cctgatggtg tcctcacccg gcaaatgatt
     8221 tcggatccca cgggtcaaat catgcgggag cgggtaatgg cggcccggcg gtcgttgcca
     8281 cccaacgtgg cccgctttag cacattgcga aaggtctccc taccgcgttt ggaaaaggcc
     8341 attgtagcca accatggcgt ggtgaccgat gagatgaagt acctggcggg acttctccgc
     8401 atccgctacg tgttcctgta tcctgaaagt ggtgacatcg ttctggccgg accggcggaa
     8461 gggtgggtcg agacacccac aggccgggtc gtgggaatca acactggccg ccctgtgctt
     8521 cagcttcaag acttggtaac ggccttgcgt gcttatcctc cggacggtga aggcgtcgat
     8581 gtcattggct gctccatcga tcccacccag gaaggtctcg cccgcatgca ggagttcttg
     8641 cgtagcctgg ggagcacgtt cccaccggga caagaggctc aggtcgccga gtatatggtc
     8701 aacggcctga aaaagagttt gggtgtccag gaagtcacaa tcctgggagt gccgccggaa
     8761 acccattttg cccatgtgtt ggttgaggcc gactatcgga tgaagctgat cggcatcggc
     8821 ttggaacgac cgcctgtgcg tttggtgagc ttcgttgaac gcgcggcttc gtccgccgtc
     8881 tcgcctaatc agctattccg ctggtacttt gtgcccgatt atcagtgcct gcgtgtgacc
     8941 gaggacggta cggccttgga attggtcggg gaaggcgtca agcttgtggg tgaagatgaa
     9001 cttgtcacgg ccagcggcga gcgaaaagca gcctccgcag ctagtgccgc gagtcgtgcg
     9061 ttcacgacca gtttcacacg gcattatccg gaactggccg caaaggtccc cgtttatgct
     9121 gagcttcgca acgtgattga catggcgatc gttgccgcct atatcaagca gcaagatttc
     9181 tacaaccagg ccaattggaa aatggaattc ctcggtcgag aagaaaactt tgcggtgaag
     9241 acgtatccag cccccaagaa ggctgagaca gccatcacgg cggtcattcg tcctggccgt
     9301 gtgagcacac ccatcggtgg tggcgtgcac attgaagcgc aacaggccct cgattacgaa
     9361 aacctcctca aggacgagaa aggtgaggtc caaaaggcca aggccagcgt caaagtggat
     9421 ttgccggaag gcgtctggtg gtgggactga tcggctgttt cagtctttaa cgtcttcagt
     9481 tgtgacttga gtctctttca tctcgggcgc atagcgctcg agatattttt ttagacggat
     9541 cttgcgtcgg gcactgtcgg tgctcatgta ccgaactagc ccaaagatgg ggcgatcggg
     9601 gccccagggg cgatcatacc ggccgaagat ccagcttatg cccgtatatg aattcggatc
     9661 gcttccgtcg aggccgtact tgttgttcag gtaaatcatc cattccagcg ccgtttgcgg
     9721 gtcaggggtc cattccagaa tctttttccc ccaaagcatg cggagataat tatgcaggcg
     9781 gccttcctca cgcagctcat tctgggccgc gttccacagg cgatcatggg tttgcgcatt
     9841 ctccaactgc tcaagggtat aagtgatagg tcgtctgtcc gaggcgtgcg ctgtgagagt
     9901 tttctttgcc cagtcgggaa gcgattcaaa acggtcgtag tcgtcgcaac gtaaacaaag
     9961 attaagcccc agttcacgcc agacgacgag ctggtcaagg aattgagcga tactcggcgg
    10021 aagattccac cacgtcccgg cgtctgcttc ctgcgaaggt cgatcgaacg accaacccat
    10081 ttctgtggcc cgtttgagca agtccacgat catttcaaac ggtgaaatga acccgaaatg
    10141 caaatacggg gccaacccac tgacgccgtt ttcgtcgggg tgattgcgcc actcgtgata
    10201 atgctccagt gcagtttcca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 ntctgtacga gagtgcgaaa gccgcgggct gtgggataat agcgtgagac actccgaaac
    10381 gggatcaatc ccgatgaatc gaccgcttcg attcgggcac gcgtgcgact tgccaacgac
    10441 tccaaaaaag ctctgtactc ccgatcaggg tgatcgtcca cgatgataac atcacaggtc
    10501 tggaacaagc tgtaaagata ttccacagct attcgaccaa acttgcgaga ttgtgcccga
    10561 tacaaaaatg ggcctcgctc gacgagactc tcatgctccc gcatcccttc cataacgaac
    10621 acggcgtgtc gatcagtcaa cgtttgatga aagggcaacg tttccaaaac tgtgagatcc
    10681 ttttcacggt cggccaccca ctccagggcg cgatccacgg catagttcca ggaccagcgg
    10741 cgattgatgt gcatccaata aagcaccacg ttgggggatt gatggggtgt gccttccgca
    10801 atgacacgga tgcgctcttc cggaactttg ccgccccata tcttgccgga aagaatctga
    10861 ctcgctgcct ccgcaaaggc agtagcagga gaagcgataa gccgattccc acgcattcgt
    10921 tcgttactcg gacgtcttgt tcaaatttcg gcgctgccca agatagggtt gcgcttggca
    10981 cacaatgcgc gccacgggcc actagagctt gtccaccgtg ggcaccacaa atggttcgcg
    11041 gtattcccgc gtgagcagct tgttggcgtc gggccgatcg atgaagcact cattggcggc
    11101 atccacaggc aagagggcac ccagcgtgag aggctccttc tccaaatcaa tgccattctc
    11161 cttgaggtgc tcctcgatcg cattcaaggt tgccacacag tcttcatgga cctggagttt
    11221 ttcgagtgcc gccaccaact ccttgtgccc ggccttttcc ccaagcaagt aggaaatatt
    11281 ggccaggtgc gtcaaggctg ttgagatatg cgtttcttca acgggagcat tcaacatttc
    11341 ttgtttgccg gccttcaccg cttcgacgaa gttgccgaag ttatccacat ttgggcctgt
    11401 aaggttctcg cggggttgtc cctgcttgtc atagaccacc acgttgggac caaagctgct
    11461 ggcaatgtag ccctcctcgc cgacaatgag caccgccccg ttaagaaatg gcggattggt
    11521 tttgtggttt cgcacctctt gaaccagcgt gattccgccc aggtcatgga cagccacctg
    11581 aacgttagga gtttcgcccg catcgttaaa tcccaaacgg ccaccgtaac tgatcaccca
    11641 tttgcccagc gttttgaacg ggacgatgat ccgagcggta tccacgcagt ggatgcagtt
    11701 attgccaatc tcgccattac catagtccca gaaccaatgc cagtcgtagt ggaacgactt
    11761 gcgggtgacc ttggccatgg gagccggtcc tgcccacagg ttgtaatcga cgcctggggg
    11821 aatggggtaa tcgcccggtg gcccaattgg gcttcgcggt cgatacatca cacaccggat
    11881 cactttgact ttaccaatct tgccggcttt gatagcctcg gctgccgctc gataacgacc
    11941 ttctgaccgc ctttgcgtcc cgccttggca gagcttgtta tacttttggg cagcctggac
    12001 aatgcgtcgt ccctcgctga cgttgtggct gactggcttt tccacataga catgcttgcc
    12061 ggcttggatg gcccaaatcg aggccaaggc gtgccaatga ttgggggtgg caatggagac
    12121 taaatccacg gccggatcgg cgaacacatc gcggaggtct tgtgcaaatt tcggcttgta
    12181 accgaacctg gcggcaaaac gctccacata agtctgcccc acgtcacgat cggcgtcaca
    12241 cagatagagc acatggcaat cggggcgcga agcatacgca ctggcgtgat cttgcccacg
    12301 tccccgaacg cccaacaccg ctacctgcaa tttatcgttt ggacttgtag gcgccgcagc
    12361 acctgcagat tgggggcctt gctcggcggc caccacagga agccccgccc ccaggccaat
    12421 cccaccgagc gacgctagaa tggcttcctg aaggaactct cttcgtgggg taacttgtcg
    12481 tgccatggct agtacctctt ttgttagaag atttggtaaa aagctcacca gcctcgcagt
    12541 gtctcccaga ctggaaaacc cacgtccttt tgataccgtt ttagccgcct tagtgtcgtc
    12601 ggcactgagc tcttgcgagc cactcactgt tctgacggca tttccgtctc gcagcagcaa
    12661 ggctcgtcgc ccggctccac ggtccggatg acgaggtact tcattttgtc gctaaaaaag
    12721 cgtgcctcag cgtgcatgac attcggggga aagaagacca tctcgcctgg ccccacgcgg
    12781 taagttcggc ccccgaaggt gaattcggcc tcgccttcca agatgaagac tgcttcctcc
    12841 gcggcatggg cgtgttgacc gttccaaccg tccgcgccgt tcagttccgt caggtggaca
    12901 tgcagttttc gcgcaccctg cgcctggccc agcaatggcc gatacaatgc tttcgtgttt
    12961 tccatagtca tccttcttgc aggttacaga ttttcgcagg tcgtgtcatt attttttccg
    13021 gcccagaggg caccgtcaag tccgcggcaa atgaaggacc acgctgtttc gtggtcaacc
    13081 ccagattggg ggcaaggtgc ctctctactg gtgatcaatt ctatgggtat catcaaggga
    13141 aatgcacgac gaggcctcgc atgaccgttt atgatgtcct ttttcaagtc tggtggtttg
    13201 tcctgggtgc ggtcctgggg agctttctca acgtcgtcat ccatcgactt cctcgaaagg
    13261 aaagcctcat ctggcccggt tcgcattgcc cgagctgtgg ccacctcatc cgctggtacg
    13321 acaacgtgcc tgtgctcagt tggctttttc tgcgggggcg atgccgcgat tgttcggcga
    13381 ccatttctcc gcggtatccg ctcgtggagg ccatggcggc attaacggtg ggtgggttgg
    13441 cttacgccgt aagcggcggg cgatgggggg ccctcctttc taccggccaa gggacctggc
    13501 ttgcggcgct ctttgcgccg gcaattctgc atttgctgat ctatgcgacg ttttttctga
    13561 ccctcatggc agccgctgcc atcgcgtggg accgtgcgcc ttgtcccccg ctactttggc
    13621 tcccggcatg ggtcctggca ctgatcggcg tggtcgttgg tagcaagggc cacgcaggga
    13681 cggcgttccg ccaccttacc ctgccgatgt tctcctggtt ccactggggc ttggccctgg
    13741 tcctgggaag tatcaatgaa ctgctgctga gtggctggga aagtaacccc ggcggaaacg
    13801 gcccatcttc ccaaaagcga tcgcgtaagg aacgacgcaa gaaacgacag cgggccgagg
    13861 ttaatcctcg tgatcacgag ggtcaagccg agatagaacg atcacggagt gtggacttta
    13921 tctcctttgc cggacttgcc gggatcctct ttgcaggaga gtcggccgtg gcgttggtgg
    13981 gcggactgtt gctccttgtc gtatttattc gtgcggcaag tctcttgtca tcgagccgcc
    14041 agcggccagc gggcgatccg tcgcgagtcc gggccgtcct tttgggcatc gcgtttgtcg
    14101 cgtcgtggag tgccatcctg ttggaaatga cacgctgaat tggtagcatt ttgttgacca
    14161 atccggtcac ggggcgtcga acgcttggtg tcactgactg gaaggagagg tccgtatgca
    14221 gacgaggctg ttacgttttc gccgggcctt ctgtgcgcca tggggcatta tgctggtctt
    14281 tctgctcacg gtggccggag ggaaaggggc ctctaacgtt ggcctggccg atacgccgag
    14341 tcatggcgtc gatccgcgct tggtggccat ggtagaaagc gactggcaaa agcaggaaac
    14401 gcggcttggt cgcgactgtg cttccgtgga ggcgatcaag gcagcgttgc ggcgactaga
    14461 agcggcgttg gaggagttgg agaaggcccg gttgatcccg gaagaacggc tgcgtgatgc
    14521 gccgacaaca ctgaagggct tctgggagcg gacggccctg gcctcgacac tccctgccga
    14581 ggaacggacc aaactttatc gagagattcg ctggttcgga cgcagtcttc tcttggagaa
    14641 ccggcttttt gccgaccgac cgattgtctt tttgaagaca cggcggttcc tctgccaaat
    14701 gcttcacgag tacatcgcct acttttacag tgccgcggac cttcatggcg gaggggtgta
    14761 cgttctgaag aaaccgggag agagcttgga gattgaggat ctaacaacgg gtcactttgg
    14821 ccggggtatc ttccaatcgc tcgcactttc ctatgatgcg aaaacagtgt actttgcctt
    14881 tgcgccgatc cctgtgggcg atcgacagcg accgcttcgg ccggattgga gcgtgcttac
    14941 cgccggaccg ctgccgccag aatttgcctt tgaatcgccg gaccggccgt gctttcactt
    15001 gttcgccatc gatttgacaa atcgcacgat ccgtcagctt accgatggtc gctatgacga
    15061 cacgatgccc tggccactgc cggatgggga cctcgtcttt atgtccacac ggcgcggtgg
    15121 ctactgtcgg tgtaacaatc cttgggaacc tattcaaacg tacacgttac atcgtctcaa
    15181 ccccaaaacc ggtgccatcc gcacaatttc ctatcacgaa accaacgagt ggcatccggc
    15241 ggtcctcaac gacgggcgaa tcgcttactg tcgctgggac tatgtcgatc gttcggcggc
    15301 ccatttccac ggcatttggg tcacatcacc ggatggcacc aatgcccgca gcgttttcgg
    15361 caattacacc atggagatca gcacgtgctt ccagccgcgg ccagttccgg ggtctcggaa
    15421 gatcgccttt attgccgggg cgcaccacgc tgtggtcggc ggatcacttg tgctttttga
    15481 tcccgacaag gcccggtatg atcccgaaac cggcgaagac gtgtttgact cgctggaagt
    15541 cttgacacca gagataccgt ttcccgagac gcccagcgag tggcccaccg gttacgtggt
    15601 cagtccttgg ccgctgtcag aggacttcta tatcatttcg ttcggtcatg accggctgcc
    15661 cggcatggga tcgggaaatc gttcagagtc tggggtggga atctactatt tcgatcgctg
    15721 ggggaatctg gaactccttt acgaggaccc aggatgggcc tgcttggatc ccgtacctgt
    15781 gattccccgc aggccgccgc cggtggtgca ggaaacgttt gatcccgatt tagggcagga
    15841 ggccgaagtg gtggtgtatg atgtgcgtca gagtcattac ccgctgccgc cggatcgccc
    15901 aattcgcgag cttcgcgtct ttcagctttt gcccaaggcg acccatcccg tcaataatcc
    15961 gcggatcggc cacgccaacg cagagaatgc ccgtgccctc ttggggactg tcccggtgga
    16021 agaggacggt tcggcatact ttcgcgttcc ggccgatcgt cccctttatt ttcaaattgt
    16081 cgatgatcga ggccgggcgg ttcaggggat gcgctcggtg acatacttcc gaccaggcga
    16141 acggcggggg tgcatcggat gtcacgagcc gcccaatagt tcggttccga cccgggacat
    16201 cttggcactg agacgtgaac cctctgcctt gacgcccggc ccggatggtt cgctgccgtt
    16261 ttccttcccc cggctggttc agcctgtgct tgatcaattc tgcatccgat gccactcgcc
    16321 gaatgctggt ccccagtttg ccgcccccga cttgatgggg cttccgcagg gggaatttac
    16381 gcggtcgtat gtgaaccttc ggccgtattt acgctggtac gaatggggcg gaggcagcat
    16441 ctcgcagatt gtcacgcggc ccggccaact gggggcggac gccagcccgt tgctgaatat
    16501 cctcgagaac ccgactcatc gcgaggccgt aacccttgat agggagtcgc ttttgcgatt
    16561 gaccctttgg ctcgatgcca acgtcccgtt ttatgggaca tatgacaacg accaaagggc
    16621 gcagcagcac gccgggcagt tgattgccat gccggaattg cagtagttgt gttggggtcc
    16681 gtcgccctgg tcgacgccat gacccactac tagggggcat ggcacccgcg gcttttcttt
    16741 ctttttctgc ttagccttga taccccacgc agcttggtgg attcgccttg gagaaatcgc
    16801 catttctggg taatttgcag taggtgaatc gagattgtgg tgatcggagg gggcaattga
    16861 caagttgacg atgaggagtg ggaaactatg actccggaag agcgtcaggc agccgtcgag
    16921 cgaattttga ggtcgcccag ctatcgggtg gcgtatcagg acttggattt cctgaccgat
    16981 cccaagttgc gcgccacacg gatggaattg gagttgctta agccggaatt ggcgttcgag
    17041 cgggagaaga tcaattccac cgtcgtagtg ttcggaagta cgcgaatcgt cgatgagccg
    17101 gaggcccgac agcttgtcga aaaggcggag gcggacctta aagcagatcc ggaaaatccg
    17161 aaattgcggc gagcttacga gcgggcaaga agcctcctgg aaaaaagccg ttactacgag
    17221 ctggccagac ggttcgcata tctcgtttcg caatcctgcg caaacaacgg gcaatgcgac
    17281 tatgtgattt gcacgggcgg ggggcccgga attatggagg ccgccaaccg aggggcctat
    17341 gaggccggag caaaatcgat cgggctgaat atccggctgc ccatggagca aatccccaat
    17401 ccgtacatta cgccagagtt gtgcttccaa ttccgctatt ttgccttacg aaaatttcat
    17461 tttctcctgc gggccaaggc cctggtcgtc ttccccggcg gctttggaac cctcgacgaa
    17521 ctgaccgatg ccttgacgct ccggcaaacc ggtcggatgc agccgatccc gatcatcatt
    17581 tttgggcggg aatactggga ccgagtgctg aatttccagg cccttgccga tgagggtgtc
    17641 attgatgatg aggatctaaa actttttgaa tttgttgaga cagccgagga ggcttgggaa
    17701 aagatttgtc gcttccacca cttgtcgatg ctcgaggcta agccggtcta ttgggagaat
    17761 tcggggggta ttgaccccca ctgatgatcc caaatagtag gtccagtgac cccccagacc
    17821 gcggccagtt cgacggcgtt tttgcgtttc cagataagag cctatttcaa gtctatccta
    17881 aaaagccccc ctctccctct gggatcgggt gggaatgagc gcaaacgatg gtcggaatcg
    17941 atccaaaaaa ccggcagcga ttcgagccac cctcaccccc gacccctctc ccgccgaacg
    18001 ggcgagggga gattgtggga taggctctaa gctgggggca tgagtcagac aaactttcgg
    18061 cacgtttagg tgctggccgt gggtgggcta taattgaggg a
//
LOCUS       DAOSUT010000079        17994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1973_length_17994_cov_8.868889, whole genome shotgun sequence.
ACCESSION   DAOSUT010000079 DAOSUT010000000
VERSION     DAOSUT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 17994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17994
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_17994_cov_8.868889"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PLD05_08865"
     CDS             complement(<1..1696)
                     /locus_tag="PLD05_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOP77591.1"
                     /translation="MSHFVPVGVDLGNSRIRVAYLNLQGHLEMLRDERDEVNIPNAVR
                     FKGQDILVGKDALDDGLFFSPETAIWIKRELGSRTYSRAVHGQRLPPEVIQACVLRKV
                     RVELLRQLAASPACVMGVPGYFDEVRRKAVINAGQIAGLRVIDVIDEPLAAALAYANA
                     AGALRLESPGQEETILIFDLGSSALQVALAKLSSSECRILAKDYDVELGGYDFDVTLG
                     EIVAEAMMAAGYPDPRADAATWGRVYRAVVEAKHGLSSQNRIHFVYDYEQAHFELIIT
                     REQYEAATAPLLRRIERLLGRFLERTGVSWSEIDVVLPVGGAVRTPCVVQLLTRLTGR
                     PIRFPINVDEAVARGAALYAAWKLEEARRVETKEAAPEVVTAGSATAFGERRITTFGN
                     LLPYAVGIRHPQERDEETICLVSRATPLPFKVTRRLSLGRRDKNELTLVVAKNELGSW
                     EPYGRLIVRGLPEALSPQSFLELTVECQVNGRLGFHARLFPTAEVLRTEFVVPSALEI
                     EAVERWQRTMETSLTWEGLTQTMASPASETRDSRSRDTTDDSSPVGNAKPVLSREPA"
     gene            2013..2633
                     /gene="cyaB"
                     /locus_tag="PLD05_08870"
     CDS             2013..2633
                     /gene="cyaB"
                     /locus_tag="PLD05_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOP77592.1"
                     /translation="MGNQGTTKASEMLVEVELKFRVAEMEELARQLSSLATEPEVLKE
                     ERDLYFAHPVRDFSQTDEALRLRRSGMHNYITYKGPKIDTVSKTRYELDLPLLDGEDV
                     FKQWCDLLEKLGFRPVAEVIKQRRKIWTQWEGWRVEISLDQVENVGQYVEFEIVCDEE
                     RVSQARDALLRLAESLQLTHSERRSYLELLLAERSPTHPPTTNVQG"
     gene            2704..4050
                     /locus_tag="PLD05_08875"
     CDS             2704..4050
                     /locus_tag="PLD05_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP77593.1"
                     /translation="MDRREDHHRANQHHGYNGHWCTDDTRPVFKRHRNFWLLVLHQIV
                     FRVGWTFKTESVVLPAFLDHLAGPGAGVFRGFLPVLNRLGQGILPLAASQAIEASRHK
                     RLLLAITTCLLAIPLSVFALIAVVDDELGHGWEAVSFLLVYFAFSAVYGIYQVVFNTV
                     QGKLIEPEKRGRLMSYSTFWGTFPAILAALCALRPWLRDGGARYDLVFLSAATLFAIS
                     GILCLFLREAKKTVDEETSTQSNPVKKMVTSLRCHPNLRGLWFVTTVFSISLTLTPHY
                     QAFARDHLHIGSGQLFLWVIAQSASVGLFSVLIGGLADRYGNRLTLAVLIFGSTLAPG
                     WSLALFHMPQFAETSVWLTFMALAFSTLVPRVAANYALELVPEEDHPVATALLQFGMT
                     VPLFASPLFGQFGEWWGFEPLLYGAIAVNVTATVATFVLPEPRRRIPPGKVITPEE"
     gene            complement(4099..6072)
                     /gene="argS"
                     /locus_tag="PLD05_08880"
     CDS             complement(4099..6072)
                     /gene="argS"
                     /locus_tag="PLD05_08880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOP77594.1"
                     /translation="MNVLSELRKRFSAILTDYVESPEEFLKLIHPSQDDRFGDYQADL
                     VMPLAKRLGRKPRELAEELVNRLDVSDMAHPPEIAGPGFINFRLRDEYLEQLLERLVQ
                     DPRLGVPLAEKPETIIIDYSAPNVAKPMHVGHIRSTVIGDALYRTLRFLGHRVISDNH
                     IGDWGTQFGMIIYGYRHFLNAEAYQQNPVQELARLYRLVRQLIDYHESVQKQAEIKRQ
                     LEEKEAALADLRNQLQNMTEAERKKITRKLRQLESEVAQLQDDLSEIRTKIEAVECDE
                     TLRRAAMEHTDIGQKVLQETAKLHAGDPENLKLWREFLPHSLDEMNRIYKRLGIQFDF
                     TLGESFYHDRLGALVDQLLKLGIAQESEGAICIFFPDIEAPMIIRKQDGAFLYATTDL
                     ATLCYRMETWKPDTILYVVDFRQSLHFQQLFAVARKLGFDQVKLVHVSFGTVLGEDGR
                     PFRTREGDTVGLESLLDEAVRRAAEIVNANDDAKSNGPELSPEERRRVAEVVGIGAIK
                     YADLSQNRTSDYVFSYDKMLAMTGNTGTYMQYAYARIQNIFAKGGVSAEAIRQFDGKI
                     RLKHPAERALGREILRFPEAIELVVEDYRPNQLTAYLFGLANRFSTFYENCPVLQAEN
                     AAERQSRLQLCDLTARVIRQGLELLGIEVVPKM"
     gene            complement(6174..6593)
                     /gene="rsfS"
                     /locus_tag="PLD05_08885"
     CDS             complement(6174..6593)
                     /gene="rsfS"
                     /locus_tag="PLD05_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOP77595.1"
                     /translation="MNESENLSPALRDKLSSLEKALLAARTADEHRGQDIVILDMREL
                     TTFFDFFVIATGTSRRQLHAMSEHIDEALAQCGARRLGIEGFGESRWILLDYGDVVVH
                     LFDEETREFYALEDLWAHAGRIPFTPRSSLPDAGNGV"
     gene            6876..7511
                     /locus_tag="PLD05_08890"
     CDS             6876..7511
                     /locus_tag="PLD05_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOP77596.1"
                     /translation="MMSRYDNVYVLDVMSEDRPGIVAAVSAAVEELGGNIDACSQTVL
                     GGYFTLIMIVSLPQRMSPAELAERVRQAGGNWDLQVLARAASPSLPSLTKPAHDRFVI
                     TAFGTDQPGIVRRFSQYLASKDINIVDLYGDRSGNEFVLIGQLEVPTKWDIRLLQADL
                     EQMGKDLGFTVKLQHENVFVATNQLRLFHASERWPTLGGTAQVPAQTAKTR"
     gene            complement(7724..8746)
                     /gene="gap"
                     /locus_tag="PLD05_08895"
     CDS             complement(7724..8746)
                     /gene="gap"
                     /locus_tag="PLD05_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOP77597.1"
                     /translation="MAIRVGINGFGRIGRLVVRRMMQKAGQFQIVGVNDITDVKTLAM
                     LFKYDSVHRTYPGTVSFDEKNLIIDGQKIPVMAEKDPTKLPWKDLGADIVVESTGVFT
                     SRGGDGKPGYASHLEAGAKKVVLSAPAKDNPDLTCVLGVNDDQLKPEHKCVSNASCTT
                     NCLAPVAKVLHEKFGIVRGLMTTVHAYTNDQRVLDLPHKDLYRARAAAANIIPTSTGA
                     AKAVGLVIPELKGKLTGIAMRVPVVTGSVVDLVAELKRPVEAAEINAAVKEAAEGKLK
                     GILQYTEDPIVSSDVIGNPHSSIFAAPWTQVMEGSMVKVVAWYDNEWGYSCRTVDLVE
                     RLARLL"
     gene            8863..9666
                     /locus_tag="PLD05_08900"
     CDS             8863..9666
                     /locus_tag="PLD05_08900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP77598.1"
                     /translation="MRLLAAYRPTWLLPSGVPRGVWEYAHSEDIAAEYDESIASTDLV
                     VFDQQVIARYCKLPGLVVDLGCGTGRALIPLLKRGFRGVGVDLSRPMLQAFTEKAKQN
                     GVSVWAIQANIVELDCLQGGIADYCLCLFSTIGMIHGRSNRRRVLEHVRRILKPGGVF
                     IVHVHNVWFQALTAGGRSWLIPHWFQTRILRKGQFGDRFFEYHGIPNVFVHAFTRREF
                     LRDLRSAGLCVRKLIPLAVGRREPLAHSWFFGWLRANGWIAVCERPTET"
     gene            complement(9795..>10667)
                     /locus_tag="PLD05_08905"
     CDS             complement(9795..>10667)
                     /locus_tag="PLD05_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HOP77599.1"
                     /translation="MRSAFGPQRSVISCSIDPTAEGIRKARSFARSRQGGVDPARFSA
                     ALEQLLGNQVVTVTGVPENTHFARVLVAADFRMKQISIGLEPAPIAGLPSFPTLVSSS
                     GRVPENTMPRWWLAPDYEPLLRDETGLTWKFQKASVKTMVEADLRGATGEREKIVEAA
                     PVYRKWAELMTARYEELSKADPVFGQVRNCMDLATVAALIASEGLLEKADLALPTILG
                     NDGVKPAELAPPKYVPAKSVFGRVGRNWIFVTGGVEINAWELASRQSVSAELAIWRQQ
                     LANPSTENWWYDAK"
     assembly_gap    10668..10767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10768..10976)
                     /locus_tag="PLD05_08910"
     CDS             complement(<10768..10976)
                     /locus_tag="PLD05_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77600.1"
                     /translation="MVSLWGRFGWMKSLTAVAAFAVLIVGASHGIAQTTGVYANRAVG
                     GVMIDPSGFIQKAPSGSLAEIRQLW"
     gene            11645..13678
                     /locus_tag="PLD05_08915"
     CDS             11645..13678
                     /locus_tag="PLD05_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOP77601.1"
                     /translation="MSEVAYLVIREGANWTDVFRLVPGQVITIGRAPTNQIIIKDERC
                     SRSHAEVFVSEGRWVIRDLESRNGTIVGNKLIKGDHPLESGDIIRIGRSQLVFVRRLS
                     DAFPETGTVPGREPVVQEAGGQSANVSDSDVEVLGEYEPTTITHRRNRARLLESEERE
                     DAVLAEAGKAAAKLCRLAFELGKATTAVRMAELALEGLFEMTAVDGGAVLLVPRDFSG
                     VATGSDLRVVASKTTSRWPYHRVSNFLASTVLREGEAVLARNVLDDSAIGSRDSRGEI
                     HATSVICAPIRQGKRAVGVIHLYSTDPNRGPTPEDLEFTLAVADTLAVALTNLNRQEE
                     LAENLTQVKTENVQLRERLGVQSELVGRSEAIRKINEEIARAAATKATVLIRGESGVG
                     KELVARAVHFSSSRRAGPFVCINCAALSEELLASELFGHEKGAFTGAFERKIGKFELA
                     HQGTLMLDEIGEMSPGIQAKLLRVLEGHAFERVGGSQPIKVDVRVIAATNRDLEQEVA
                     QGRFRRDLFFRLRVLEIVVPPLRKRIEDIPELAYYFLAKFREETGRKIKGFTKQAMER
                     LTSYRWPGNVRELKNVVERAVVLCKGEYIDVDDLLLSTLPTTGDTAEALLQDRGYRPC
                     SLEELERAHILATLRHTNWNKSRSAHILGIERSTLERKIRRYGIDELARKNPE"
     gene            complement(13849..>14637)
                     /locus_tag="PLD05_08920"
     CDS             complement(13849..>14637)
                     /locus_tag="PLD05_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP77602.1"
                     /translation="LSGFAVEFVGHREYVLGDDPKYIDWRVYFNRGRYVVKQYAMETN
                     LVCHFILDSSASMRYGEGEQQKLQYAARAVVTLGYAILRQGDKVSLSTFDDEIRGFVP
                     PSHSLAQVVRMTEHLDMTEATRKTRMAECLYELAGRFHRREILIVLSDFFTDLGELEK
                     AVQQIRAHRHEVIFLQILHHDEIAFEFNGPTKFVGLEQPDELITRPEELRRHYLRAFE
                     QFQGRLVDLCQRNRVEHVLVDTSRNLADVLADYLHFRNRIRRLR"
     assembly_gap    14638..14737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14738..15630)
                     /locus_tag="PLD05_08925"
     CDS             complement(<14738..15630)
                     /locus_tag="PLD05_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOP77603.1"
                     /translation="MQEQVSQSDLEAVEKCHQAYLRIRQELAKVIVGQERVIEEVLIA
                     ILARGHALLEGVPGLAKTLLVSSLAQATHLTFRRIQFTPDLMPSDVTGTDIIQEDPKT
                     GERGYKFLPGPIFANMILADEINRTPPKTQAAMLEAMQERQVTAGGRVYRLPDPFFVL
                     ATQNPLEQEGTYPLPEAQLDRFLLYIKVDYPSAAEEWEIARRVTGGQQGTIEPLMTGE
                     ELIQYQKLVTRIPISDQVLGYAWALVRASRPGSMESPDFVTRWVAWGAGPRGLLTLVT
                     CAKARAVLHGRCHATIGDVQAL"
     gene            complement(15635..>17994)
                     /locus_tag="PLD05_08930"
     CDS             complement(15635..>17994)
                     /locus_tag="PLD05_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77604.1"
                     /translation="LLERVRRFQGEMESDSRDAARRIIEALAERQADLREKTLNAEAN
                     PQEVQTLTENQLQLQKEIEQAAKKLPEGQEALNQAAEAARQATETLFQGQTQQATEHQ
                     QSVIDHLAEALTTLQRESPTGEFTGLPNSEAVRSQLADLRQAKVELQEIREEQATVSQ
                     TARQGELEEAAKGETRLSQKINEVPTKKSLPQDVTEAIRKAAETVGQSAQALSELSKT
                     QTKPSGQEGQGEFSNQAHPDHPPEAGKDDQNTTASSGENRPQVEKPPKAEGSHEDHAV
                     RMATRRAEQAIDEAISAVEMAIADAERARLAAELVDAVQSMVGSEGQNPDRANLARQR
                     AAELEQLTQQQLDQVRSTKKAVEEELPLARDPASQALQQLEKVASQAIEAAALQQEAV
                     GREDLAKQIRSAGDMKKAIALAKEAAEHSDESNSSPAKNQSIPLQEKVTEAAMEALAE
                     LARLPSLPEDVNPRGRDAARAALDSAKSASQEAAKRLATPPNGENDGAADLQRKAASN
                     LATAAEQIHGLMESLASHVADKFGQHAQKAENLSEMAVPVHPEATSNLHMAENAAHQG
                     STEAGQRLGAVTQGHQGVEHGLTTAVEALAERENQLSRALSAAQNIPELLNALERQPN
                     SVATQPSTPGQNDNPMDPAEKAMAAILAAAQQAATPQAGHPSTSHTAEDSPSTPASQA
                     ATQPSNSASADSQGGFAKGRTLESQNVASTARPLQQQTSQPNADSRGAYQQGQGVSTQ
                     PGEANLPPWLLQLPPQMREAIRSGMTLPPPKGYEDRLRRYFQNLE"
BASE COUNT         3747 a         4883 c         4956 g         4208 t
ORIGIN      
        1 gggctggctc acgactcagc acgggtttcg cgttccctac aggcgaagaa tcatcggttg
       61 tgtcccttga ccggctgtca cgtgtctcac tagcaggact ggccattgtc tgggttagcc
      121 cctcccaagt taaggatgtt tccatcgtcc gttgccaacg ttcgacagcc tcgatttcga
      181 gggcggaggg aacaacgaac tctgtcctaa gaacttcggc cgtaggaaag agccgggcgt
      241 gaaatcccag ccgaccgttg acctggcatt ctacggtcag ttccaagaat gattggggcg
      301 agagagcctc cggaagcccc cggacaatga gccgcccgta aggctcccat gacccaagtt
      361 cattcttggc aacgaccagg gtcaactcgt ttttatcgcg ccgtcccagc gataagcggc
      421 gagtcacttt aaagggaagc ggtgtggccc gtgagacaag acaaatcgtt tcctcgtctc
      481 gttcctgagg gtggcgaatt ccaacggcat aaggaagcaa gttcccaaac gtggtgatcc
      541 gccgttcccc gaacgcggtg gcacttcctg cggttaccac ctcgggtgcc gcctctttcg
      601 tctcaacacg ccgggcctct tcgagtttcc atgcggcata taacgccgct ccccttgcaa
      661 cggcctcatc gacgtttatg ggaaagcgaa ttgggcgacc cgttaatcgg gtcaacaatt
      721 ggacaacgca gggcgttcgg acggccccac cgacaggcaa aaccacgtcg atttcggacc
      781 acgagactcc cgtgcgttcg agaaatcgtc ctaaaagacg ctcaatccgc cgcagcaacg
      841 gggctgtggc cgcctcatat tgttccctcg tgataatcaa ctcaaaatgt gcctgttcgt
      901 agtcataaac gaaatgaatg cggttttgtg acgacagccc gtgtttggct tccaccaccg
      961 cgcgatagac ccgtccccat gtggcggcat cggctcgggg atcgggatat ccggctgcca
     1021 tcatggcctc ggccacaatt tcgcctagcg tcacgtcaaa gtcgtaacct ccgagctcga
     1081 cgtcgtagtc tttcgcaaga atccgacatt ccgaggacga aagttttgcc agtgcaacct
     1141 gcaaggcgct actacctaag tcgaagatga ggatcgtttc ttcctgtcct ggtgattcga
     1201 gccgcaaagc ccctgctgca ttggcgtatg caagggctgc cgcaagaggc tcatcgatca
     1261 cgtcgatgac gcggagccct gcgatttgac cggcattaat gacagccttc cggcggacct
     1321 catcgaagta accgggaacc cccatgacac atgctggtga cgcggctaac tgacgaagga
     1381 gttcgacgcg aaccttgcgc aagacacacg cctggatgac ctcgggcggt agccgttggc
     1441 catggaccgc acgactatag gtcctcgaac caagctcccg tttgatccat atagcggttt
     1501 ccggtgaaaa gaataagccg tcgtccaatg cgtcttttcc tacgaggata tcttgaccct
     1561 tgaaccgtac ggcattcggt atgttcactt cgtcccgttc atcacgtaac atttccaggt
     1621 ggccctgcaa attaagatag gccacccgaa tgcggctgtt tccgagatca acaccaactg
     1681 ggacgaagtg gctcatagag accacacggc gggaaaatct tctgctattt ccaagagatc
     1741 attgccatac ctgacaacca cgtgctccag tccacaacaa ggtcgtttcg gatgatcctt
     1801 taccagaccg gagaataacc tcagcctaag tctcattgta accgacggcg tctcgctccc
     1861 caaagtcagc ttcctttcct gctccgcggt gtaggtttat gcagcacgtt ttggtatgat
     1921 tcacgtgagt tagacgtaag cgagatatgt acgttgcgat tcttgggcga gtaacatcgt
     1981 gctgcccggt tttgcgatgg aagatatcaa caatgggcaa tcagggaacc acgaaggcgt
     2041 cagagatgct cgttgaagta gaactgaaat ttcgcgttgc cgaaatggag gagttagcca
     2101 gacagctttc ctccttggct acggaacccg aggtcttgaa agaggaacgc gacctttatt
     2161 ttgcgcatcc agtgcgcgac ttctcccaga ccgatgaagc tcttcgatta cgccgctcgg
     2221 ggatgcataa ttacatcaca tacaaaggac ccaagatcga cacagtttcg aagacccgtt
     2281 acgagcttga tttgccttta ttggatggcg aagatgtttt caagcaatgg tgtgatctgc
     2341 tagagaaact tggcttccgt cccgttgccg aagtaattaa gcagcgccga aagatatgga
     2401 cacagtggga ggggtggcgg gttgagattt cacttgatca agtcgagaat gttggccaat
     2461 acgttgagtt cgagatcgta tgtgacgaag agcgagtgtc ccaagcccgc gatgccttgc
     2521 ttcgattggc cgaatctctc caacttaccc attcggagcg gcgcagttat ctcgaactat
     2581 tgttagctga aaggtctccg acccaccctc caaccacgaa cgtgcagggg tagtaggcca
     2641 tcctgtgagc cgctgtgtga cggaacgtgc gattctgcat caacttgcga ttgtcaaaaa
     2701 ccgatggaca ggcgcgagga ccatcatcga gcgaatcaac atcatggcta taacggtcat
     2761 tggtgcaccg acgatacaag gcccgtcttc aagcgacatc ggaatttttg gctcctggta
     2821 cttcaccaaa tcgtttttcg agttggttgg acgtttaaga cagaaagtgt tgttcttccg
     2881 gcgttcttgg atcacctggc gggacctggg gccggggtct ttcgcggctt cttgcccgtg
     2941 ttaaaccgat tggggcaggg tattttgccc ttggcggctt cgcaagccat tgaggcttcc
     3001 cgacacaaaa gactgcttct agccatcacg acgtgtctgc tagccattcc gctgagtgtt
     3061 tttgccctta tcgccgtcgt ggatgatgaa ttgggtcatg gttgggaagc cgtatctttt
     3121 ctgctggtat attttgcatt ctcggccgtg tatggcatct atcaggttgt cttcaacacg
     3181 gtgcagggaa agctaatcga gcccgaaaaa cgagggcgat tgatgagtta ctccaccttt
     3241 tgggggactt ttccggcgat cttggccgcc ttgtgtgctc ttcggccttg gttgcgcgat
     3301 ggcggagcgc gttatgacct tgtttttttg tcggcggcca cgttgtttgc tatttccgga
     3361 atcttgtgcc tatttttgcg tgaagcaaaa aaaacggttg acgaagaaac ctcgacacag
     3421 tccaacccgg tgaaaaaaat ggtgacatcc ctcagatgcc accctaattt gcgcggctta
     3481 tggttcgtca cgactgtctt tagcattagc ctgacactga cgccccatta tcaggccttt
     3541 gctcgggacc atcttcacat aggatcgggc caattattcc tttgggtgat tgcccaaagt
     3601 gctagtgtgg ggctatttag tgtccttatc ggtggcttgg ccgaccgtta tgggaaccgt
     3661 ctaaccttgg ccgtcttgat ctttggttca acgttggcgc caggctggtc attggcactc
     3721 tttcacatgc cacagtttgc ggaaacttcg gtgtggttga cgtttatggc tctcgctttt
     3781 tccacactgg tcccccgtgt ggcggcgaac tatgccctgg aactcgttcc ggaagaagat
     3841 cacccggtgg ccacggccct ccttcagttc ggcatgaccg taccactgtt cgcgtctccc
     3901 ctctttggtc aatttggcga gtggtgggga tttgagccgc tgttgtacgg tgccattgcg
     3961 gttaacgtca ccgccacggt agcaacgttt gtcctcccgg aaccacgacg tcgaatacct
     4021 cccggaaaag tgatcacccc cgaggaataa ctggaaaacg ctggaaacta agggacgcac
     4081 cacaccttgt ggccgcgttc acatcttggg gaccacctca attcctagta gttccagtcc
     4141 ttggcggatg acacgcgccg tcaaatcgca cagttggaga cggctttgac gctccgcggc
     4201 gttttctgcc tgcaaaacgg ggcagttttc atagaaagtt gaaaaccggt tggccaggcc
     4261 gaagaggtac gccgtcaact ggttgggccg gtaatcctca acgaccaatt cgatagcctc
     4321 cggaaatcgg aggatttccc gtcccagtgc cctctcggcc ggatgcttga gacgaatctt
     4381 gccatcgaac tgtcgtatag cctctgcgct cactcccccc ttggcgaaga tattttgaat
     4441 tcgggcataa gcgtattgca tgtaggttcc cgtattgcct gtcatggcta gcatcttgtc
     4501 gtaactgaag acgtagtcgc tggttctatt ttgagaaagg tctgcgtact tgatggctcc
     4561 gatgcccacg acttcggcga cgcggcgtcg ttcctcggga gaaagctccg gaccattgga
     4621 cttcgcatcg tcgttagcgt tgacgatttc ggccgcacgg cgcacagctt cgtcaaggag
     4681 agactccagt ccaaccgtat caccctcccg cgtgcgaaac ggccgtccat cctcacctaa
     4741 gacggtgcca aaactgacgt gcacaagttt cacctggtcg aaccctagtt tccttgccac
     4801 ggcaaataat tgctgaaaat ggagactttg gcggaagtcc acaacataga ggatggtatc
     4861 cggcttccat gtttccatac gatagcaaag cgtggccaag tcggtcgttg cgtaaaggaa
     4921 ggccccgtcc tgtttgcgga tgatcatggg ggcttcaata tcggggaaga aaatacagat
     4981 agccccctca ctttcttggg caataccaag tttgagtaac tgatcgacca gggccccaag
     5041 ccgatcatgg taaaaacttt cgccaagcgt gaaatcgaac tgaatcccga gccgcttata
     5101 gatgcgattc atttcatcga ggctgtgtgg caggaattcc cgccataact tgaggttttc
     5161 gggatcgccc gcatggagct tggccgtctc ttgaagcacc ttctgcccga tgtccgtatg
     5221 ttccatcgcc gcgcggcgca aagtctcgtc gcactccacg gcctcaatct tggtgcgaat
     5281 ctcactcaga tcatcttgta actgagcaac ctccgactcg agctgccgga gtttcctcgt
     5341 gatcttcttt cgttctgcct ccgtcatgtt ctgcaactga tttcggagat cagcgagagc
     5401 agcctctttt tcctcaagct gccgtttgat ttctgcctgc ttttgaacac tttcgtgata
     5461 atcgatcaac tggcggacga ggcgatagag gcgtgccagt tcctgcaccg ggttttgctg
     5521 ataggcttcg gcattcaaga aatgacggta tccgtaaatg atcattccga actgtgtgcc
     5581 ccaatctccg atgtgattgt cactgatgac gcgatgccca agaaagcgaa gtgttcggta
     5641 gagtgcgtcc ccgatgaccg tggaacgaat atgaccgacg tgcatgggct tggccacgtt
     5701 aggagccgag taatcaatga tgatggtctc tggcttctcg gccaggggaa cgcccagcct
     5761 gggatcctgt acaagtcttt cgaggagttg ctccaaatac tcatctcgaa gccgaaaatt
     5821 aatgaagccc ggcccggcaa tttccggtgg atgggccatg tctgagacat ccaaacggtt
     5881 caccagttcc tccgccagtt cccggggctt tcggcccaat cgcttcgcta gcggcatgac
     5941 taggtcggcc tggtaatcgc caaagcgatc atcttgactg gggtgaatca gtttcaggaa
     6001 ctcctctggg gattccacgt agtccgtaag aatggccgaa aaacgcttcc gtaattcact
     6061 caatacgttc atcgactaat ccttatctcg gttgtatcct ccaagaacga tcctaagaga
     6121 caaagcaagt gtgacacgca ggggatgaga atggcaagga ccctgagggc cggtcaaacg
     6181 ccgttccccg cgtctgggag cgaagatctg ggagtgaagg ggatccgccc ggcgtgtgcc
     6241 cacaagtctt ccaaggcgta aaattcccgt gtctcttcat caaagaggtg aacaacgaca
     6301 tcgccgtaat ccaggaggat ccagcggctt tcaccgaacc cctcgattcc caatcgccgc
     6361 gcaccgcact gagcgagggc ctcgtcaatg tgttcgctca tggcgtgaag ctgacggcgg
     6421 ctcgtcccgg tagctatcac aaaaaagtca aaaaacgtag ttaattcccg catgtccaaa
     6481 atgacaatat cctgcccacg gtgttcgtcg gccgttcggg cggccaaaag ggccttttcc
     6541 agggaggaca gtttgtctcg cagggctggt gacaggtttt ctgattcgtt cacgtatcca
     6601 ctctccatag ccaaaaaggt ggaaccgctc cagttatttt agttagaatc cctccgggcg
     6661 cgaacaactc gacagccgca atcgtctgac gtagcatagt cccaatttgt gtaaaatagg
     6721 ggtaagaagc tcatgtggct ccacaacagg gcccattatt atgttatgcc catgcttgcc
     6781 atcagagggc ttccatattt aacgagtaaa cgtgactacc gttcccgccg ccgataacgg
     6841 ggcgaagcag attctgttcg agaggacaag agcctatgat gagtcgatat gacaacgtgt
     6901 atgtcttgga cgtcatgtcg gaagaccgac ccggcatcgt ggccgccgtc agcgcggccg
     6961 tcgaggaatt ggggggcaat atcgacgcgt gcagtcagac cgtgcttggc gggtacttta
     7021 ccctcattat gattgtcagc ctgccccaac ggatgtcgcc ggcggaactg gcagaacgtg
     7081 tgcgacaggc aggcgggaac tgggaccttc aggtcctggc ccgcgcggca tcgccatcat
     7141 tgccttcttt gaccaaacca gcacacgacc gatttgtaat cacggccttc ggaacggacc
     7201 agcccggcat tgtcaggcgg tttagtcaat acctcgccag caaagacatc aatatcgtag
     7261 acttgtacgg tgatcgctcc ggaaacgagt tcgtcctgat tgggcaactt gaggtcccta
     7321 caaaatggga tattcggctc ctccaggcgg acttggaaca aatgggaaag gacctgggct
     7381 ttacggtcaa gctgcagcac gaaaatgtct tcgtcgctac caaccagttg cggctgtttc
     7441 atgccagtga acgatggccg actcttggtg gtaccgcaca agtccctgca cagacggcaa
     7501 agacacggtg agacgctaac gggtacacga gaaaagggct ctccaggacg tgagatttga
     7561 ccgcggcgca aaaacgcggc cgttaagaac taattacagg ggctggaaaa agaaagaggc
     7621 gactacttgt aagtagtcgc ctcataaagt ttgctgttgt gccggaactg tgcccaccta
     7681 tcggttcaag agtcgctccg aaccacttcc ggcgcagtga ccatcagagc aacctggcaa
     7741 gccgctccac cagatctacc gttcggcaac tgtaacccca ctcgttgtcg taccacgcca
     7801 cgactttcac catcgaacct tccatgacct gcgtccaggg agcggcaaag atcgagctgt
     7861 gtggattgcc aataacgtca ctggagacga tggggtcttc cgtgtattgg aggatcccct
     7921 tcagtttgcc ttcagcagct tccttgaccg ccgcattgat ttctgcagcc tccacgggac
     7981 gctttaactc ggccacgagg tccacaacac ttcccgtaac caccggcacc cgcatggcga
     8041 taccggtcaa tttccctttt agttccggaa tgaccaatcc aacggccttt gccgcacccg
     8101 tcgaggtcgg aataatgtta gctgctgcgg cccgggcccg ataaaggtcc ttgtgaggca
     8161 aatccaacac gcgctgatcg ttcgtgtacg cgtgaacggt cgtcatcagg ccccgcacga
     8221 taccgaactt ctcgtgcaac accttggcga caggtgcgag gcagtttgtc gtgcaactgg
     8281 cgtttgaaac gcacttatgc tcgggtttca gttggtcatc attgaccccc aaaacgcacg
     8341 tgagatcggg attgtcctta gctggagcac tcaaaacgac ctttttggcg ccggcttcaa
     8401 ggtgagaagc gtaaccgggc ttgccatctc cgccgcggct tgtgaagacg cccgtgctct
     8461 cgacgacaat atccgcaccg agatccttcc aaggcagctt ggtcggatcc ttttctgcca
     8521 tgacggggat cttttggcca tcgatgatca gattcttttc atcgaagcta acggttcccg
     8581 gataggtccg atgcacactg tcatacttga agagcattgc gagcgtcttg acgtcggtaa
     8641 tgtcattcac gccgacgatt tgaaattggc ccgccttctg catcatccgg cgaactacga
     8701 gccggccaat ccgcccgaaa ccattgattc caactcgaat agccacggct ttctcctttc
     8761 ccttaaagca accacagaac gcgtattgtg ttcgtcttcc gcagagttaa aaggcttatt
     8821 atattggaca atgttactaa tgacaaccac cgggagcgtg acgtgcgatt actggcggct
     8881 tatcggccaa cctggcttct tccaagcggt gttccaagag gtgtctggga atacgcccat
     8941 tccgaagaca tcgcggcgga gtatgacgag tcgattgcct ctaccgacct ggtggttttc
     9001 gatcagcaag tcatagccag atactgtaaa ctcccggggc ttgtggtaga tttgggctgt
     9061 ggaaccggcc gggcgttgat cccgctcctt aagcggggtt ttcgtggggt tggtgtcgat
     9121 ttgtcgcgtc cgatgttgca agccttcacg gaaaaggcca agcagaatgg tgtgagtgtt
     9181 tgggcaattc aagccaacat cgtcgagctg gattgccttc aaggcggtat tgccgattac
     9241 tgcctgtgtt tgtttagcac gatcggaatg attcacgggc gatcaaatcg ccgccgtgtt
     9301 ttggagcatg tgagacggat tctcaagccc ggcggggttt ttatcgtgca cgtgcacaat
     9361 gtatggtttc aggccttgac cgccggggga cggagttggc tcatcccgca ttggttccaa
     9421 acgcgcatcc tgagaaaagg ccaattcggc gatcggtttt ttgaatatca cggcataccg
     9481 aatgtgtttg tccacgcctt tacacgccgc gagtttctgc gtgatctccg ttctgccggg
     9541 ctgtgcgtgc ggaagctcat ccctctggcg gtaggccgtc gcgaaccgct cgctcactct
     9601 tggtttttcg gctggctacg agctaacgga tggatcgctg tgtgtgaaag acccaccgag
     9661 acataatttg gcgtattcca ccgtggatgt ggtcgttagg aaaaaaagca ggacgccagt
     9721 ttcggcgtcc tgtagtgggg atttcaattc tcttaaaaga accgctgatc gaatcgaagt
     9781 ccgtcaccaa ataattactt ggcgtcgtac caccagttct cggtggatgg gttcgccaac
     9841 tgctgtcgcc agattgccaa ctccgccgag acagactgcc gagaggccaa ctcccaggca
     9901 ttgatctcga caccgccggt gacgaatatc caattgcggc caacacgacc gaagacagat
     9961 tttgccggga catactttgg aggggccaat tctgccggtt tgaccccatc gttccctagg
    10021 attgtcggca aagccaggtc tgccttttcg aggaggcctt cgctggcgat cagggcagcc
    10081 accgttgcca agtccatgca gttccgaacc tggccgaata cgggatcggc ctttgacagt
    10141 tcctcatacc gggccgtcat tagctcggcc cattttcgat agaccggagc tgcctcgacg
    10201 atcttttccc tttcacccgt cgcccctcgc agatcagcct cgaccatcgt cttgacactc
    10261 gccttttgga acttccaagt taaaccggtt tcgtctcgca ggagcggttc gtagtctgga
    10321 gccaaccacc agcgaggcat cgtgttttcg gggacacgcc ctgaactgct aacaagtgta
    10381 ggaaagctcg gtaagcccgc aatcggggcg ggctcgagcc ctatggaaat ctgcttcatg
    10441 cggaagtcag cggccacaag gacacgggca aagtgggtat tctcggggac tcccgtcaca
    10501 gtcaccacct gattacccaa aagctgttcc aaagcggcag aaaaacgagc gggatcgacc
    10561 ccaccctgcc gcgatcgggc gaatgaccgt gcctttcgga tcccctccgc cgtgggatcg
    10621 atcgagcaac taatgacgct cctttgaggg ccaaacgccg accgcatnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnntgc caaagctgac ggatttcggc taagctaccg
    10801 ctgggtgctt tttggatgaa cccggacggg tcgatcataa ccccaccaac ggcgcggttt
    10861 gcatataccc ctgtcgtctg ggcgattccg tggcttgcac ccacaattag caccgcgaag
    10921 gccgccactg ccgtgagaga tttcatccag ccgaaccgac cccataaact gaccataagt
    10981 gtctcccaat cggaaaaccg tgtttctccg cgaagatagg tcggagccaa acaaccgaat
    11041 tctgccgaaa accctgccag ggtttctcgg cgtttgaata tcacaatccc attttaggct
    11101 aattcagaga cacgcacttt tcaacctaaa tcctcagttt ttcccaaacc gtcccatttc
    11161 tgtagttcgg gtgtccaggt gccggattcc aagttgtaga acctgccgag cggatttctt
    11221 gcaacgcggg cacgacgaat catcggccat atttagtcgt ttcggcaggc catcaagttc
    11281 cggcctggat tcgagaagag tcgcaccgtc gtgtcagccg tagtatgagc cgctgtgggt
    11341 cgggctgtcg gccgcgcggt ggttggaaga tgcatggcgc attaacaccc caatcttaca
    11401 atggggcgtg tgatggccgt caccaggcag ttcgatcgcc aggcgcaacc atcgtggttg
    11461 ccctttggtg aaattcgtaa gacgctcgcc gttgtgtccg ctatagctcg acagagcgtc
    11521 cacaactctt tcggccaggg cctgagtggg taaaatagat ggtgggatgg taatggccag
    11581 cctagggcaa gggaaggggt tgtttggctc tttgcgggaa acgactgcct gagggggaac
    11641 gacgttgtca gaggttgcat atcttgtcat tcgggaaggc gcgaattgga ccgacgtctt
    11701 tcggttggtc cccggacagg tgattaccat tggtcgcgca cccaccaatc aaatcattat
    11761 caaagacgag cgatgcagcc gtagccacgc ggaggtcttc gtttccgagg ggcggtgggt
    11821 cattcgggac ctggaaagcc gtaacggtac catcgttgga aacaagctga ttaaaggtga
    11881 tcatcccctc gaatcggggg atatcatcag gattggccga tcccaattgg tcttcgttcg
    11941 gcgattgagc gatgcctttc cggagactgg caccgttccc gggcgtgaac cggtggtgca
    12001 ggaggccgga gggcagtctg cgaatgtgtc agatagcgac gtagaggtcc tcggtgaata
    12061 tgagcccacc acgatcacgc atcgccgaaa tcgtgcccgg cttctcgaat ccgaagaacg
    12121 tgaagacgcc gttctagccg aggcaggcaa ggcggctgcg aagctgtgtc ggctggcatt
    12181 cgaattggga aaggcgacca cagccgtgcg gatggccgag ctggctctgg aaggcttatt
    12241 cgagatgacg gctgtcgatg gcggtgccgt tctcttggtg ccgcgggatt tctcaggggt
    12301 ggcgacggga agtgatcttc gcgtggtggc atcgaagacg acctcgcgat ggccctatca
    12361 ccgcgtttcg aatttcttgg caagcacggt ccttcgtgag ggcgaggccg ttttggccag
    12421 aaatgtcctt gacgatagtg ctatcggaag tcgagatagc cgcggcgaaa tccatgccac
    12481 gagtgtcata tgtgccccga ttcggcaggg taaacgggct gtcggggtaa tccaccttta
    12541 ttcgacggac cccaatcggg ggcccactcc ggaagacctg gagttcacat tagccgtggc
    12601 cgacacgttg gctgttgcct taacgaattt gaatcggcag gaagagctgg ccgagaacct
    12661 cactcaggtc aagacggaga acgttcagtt acgggagcgg ctgggcgttc aaagcgaact
    12721 tgttgggcga agtgaggcca tccgaaagat caatgaggaa atcgcacgag cggccgccac
    12781 caaggccacc gtgctgatcc gcggggaaag tggtgtgggt aaagaactgg tggcccgggc
    12841 ggtccatttc tcaagctcgc gtcgcgccgg tccctttgtg tgtatcaatt gcgcggcgct
    12901 ttctgaggaa cttcttgcca gtgaactatt tggacatgag aagggtgcct tcactggggc
    12961 ttttgaaaga aagatcggga agtttgagct ggctcaccag gggacgctga tgctcgatga
    13021 aatcggggaa atgagtcccg gaattcaggc caagttactc cgcgttctcg aggggcacgc
    13081 cttcgagcgg gtcggcggta gccagccgat caaggtagat gttcgggtga ttgcagctac
    13141 gaatcgggat ttagagcaag aggttgctca ggggcggttt cggcgagatt tgttctttcg
    13201 gcttcgcgtg ttggaaatcg ttgtcccacc tttgcgaaaa cggatcgaag acattcctga
    13261 gctggcgtac tatttcttgg ccaagttccg cgaggagacc gggcggaaga tcaaaggctt
    13321 taccaagcag gccatggaac ggctgacctc ttatcgttgg cctggaaatg tcagggagtt
    13381 gaaaaacgtc gtcgagcggg ccgtggtcct gtgcaagggt gaatatattg acgtcgatga
    13441 tctgctgctt tcgaccttgc ccacaacggg agataccgcg gaggccttgc ttcaagaccg
    13501 cggctaccgt ccctgttcgc ttgaggagtt agagcgggca cacatcctgg cgaccttgcg
    13561 gcatacaaat tggaacaaga gccgttccgc ccacattctc ggcattgaac ggtcaaccct
    13621 cgaacggaag atccgccgtt acggaattga tgaattggct cggaagaatc cggagtgagt
    13681 tttccgaaaa ctcggcgagt tgggaactta ttcgggacgc ctgatgcaaa acgggggcaa
    13741 ccatattttt caaggatgaa ggccaactcg gcatgtttta tcgaagactg tgtgccacgg
    13801 ttcacggcga tggagcgtcg tgtcacgttg gggaaatcac tcccccaatc aacgaagacg
    13861 tctaattcgg ttacgaaagt gcagataatc ggccaggaca tcagcgagat tcctgctggt
    13921 atcgacgaga acgtgttcga cacggtttcg ctggcagaga tcgacaaggc gtccttggaa
    13981 ctgctcaaat gcccggaggt agtgccttcg cagctcctcg ggtcgagtga taagctcatc
    14041 tggctgttct agcccgacaa actttgtggg tccgttgaac tcgaaggcga tttcgtcgtg
    14101 gtggaggatt tggagaaaga ttacctcatg gcgatgcgca cggatttgct gaacggcttt
    14161 ttccaattca ccaaggtccg tgaagaaatc gctaaggacg atcaggatct cgcgccggtg
    14221 aaatcgtcct gccagttcgt agaggcactc ggccattctg gtctttcgag tggcttcggt
    14281 catgtccaga tgttctgtca tcctcacaac ctgggcaagc gaatggctag gcgggacgaa
    14341 cccccgaatc tcatcgtcga aggtcgagag tgatactttg tcaccctggc ggaggatcgc
    14401 ataccccagt gtgaccacag cgcgggccgc atattgaagt ttttgctgct cgccttcgcc
    14461 atagcgcatg gaagcactgc tgtcgagaat gaaatgacag actaaattcg tttccatggc
    14521 gtattgtttg acgacgtagc ggccacggtt gaaatagacc cgccaatcga tgtacttggg
    14581 atcatcaccc aagacgtatt cccgatgccc tacaaactcg acggcgaacc cggaaagnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg gcttggacat cgccgatggt
    14761 ggcatgacat cggccgtgca agacggcgcg ggccttggca caagtcacaa gggtcaacag
    14821 accacgtgga cccgcccccc aagccaccca gcgggtcacg aaatcggggc tttccatcga
    14881 tccgggacga gaggcacgaa ccaaggccca ggcataaccc agtacttggt cggaaatcgg
    14941 aatgcgggtc acaagtttct gatactgaat cagctcctca ccggtcatca atggctcaat
    15001 tgttccctgt tgtcctcccg taacacgccg ggcgatttcc cattcttcgg cagcggacgg
    15061 ataatccacc ttaatgtaaa gcagaaaacg gtcgagctgt gcctccggaa ggggataggt
    15121 cccctcttgc tcgagggggt tctgtgtggc cagcacaaag aacgggtcgg gaaggcggta
    15181 cacgcggcca cctgcggtca cctgccgctc ttgcatggct tccaacatcg cggcctgcgt
    15241 cttgggtggt gttcggttga tctcgtcggc cagaatcatg ttagcaaaaa tcggtcccgg
    15301 caaaaatttg taaccccgtt ctccggtctt cgggtcttcc tgaatgatgt cggtacccgt
    15361 cacgtcgctg ggcataagat ccggggtgaa ttgtatccgc ctgaacgtca aatgcgtggc
    15421 ttgggccaac gaacttacaa gaagcgtctt tgccagcccg gggacgccct ccagaagggc
    15481 gtgtcctcga gcgaggatgg cgatcagaac ctcctcgatg actcgctcct ggccaacgat
    15541 gaccttggcc agctcttgcc gaatccgcag atatgcctga tgacactttt cgacggcttc
    15601 caaatcgctt tgggacacct gctcttgcat gtcttcactc caagttctga aaataccggc
    15661 gcaaacgatc ctcatatcct tttggtggtg gtagagtcat cccactacga atagcttctc
    15721 gcatctgggg cggtagttga agcagccaag gcggcaggtt cgcttctccg ggctgggtac
    15781 taaccccttg tccctgttgg tatgcccccc tgctatcggc attgggttga cttgtttgtt
    15841 gctgcagagg gcgggccgtg ctggcaacgt tttgggactc caatgttcta cctttggcga
    15901 aaccgccctg actatcggcg gaggcactat ttgacggctg agtcgctgcc tggcttgccg
    15961 gcgtggaggg cgaatcctcg gcggtgtgtg atgtgctagg atgccctgcc tggggtgtcg
    16021 ccgcctgttg agcagccgcc aagatcgctg ccatcgcctt ttccgcgggg tccattgggt
    16081 tgtcgttttg acctggtgtt gatggctggg ttgccacaga gttgggctga cgctccaacg
    16141 cgttcagcaa ttctggaata ttttgggcgg cagagagggc acgtgagagc tgattctctc
    16201 gttcggccag ggcttccacc gcagtggtca aaccatgttc gacgccctga tgtccttgag
    16261 tcactgcacc gagacgttgt cccgcttcag tgctgccctg atgagcggcg ttttccgcca
    16321 tgtggagatt ggaggtcgcc tcgggatgaa cgggcaccgc catttcggac agattctctg
    16381 ccttttgcgc atgttgccca aatttgtcgg ccacgtggga agccaacgac tccatcaaac
    16441 catggatttg ttcggcggct gttgccagat ttgacgctgc ctttctttgc aagtctgccg
    16501 ccccgtcatt ctccccattc ggcggtgttg cgagacgttt tgccgcttcc tgggacgcgc
    16561 tcttggccga gtcaagtgct gccctggccg catcacgccc gcggggattc acatcctcgg
    16621 ggagactggg caatcgagcc aactccgcca gggcttccat cgcggcctca gtcaccttct
    16681 cttgaagggg aatactttgg ttcttcgcag gtgatgagtt ggattcgtct gaatgttctg
    16741 cggcctcctt ggccaaagca atggcctttt tcatgtcacc ggccgaacgg atttgtttag
    16801 cgaggtcctc acgtcccacg gcctcttgtt gcagggctgc ggcttcgatg gcctgtgatg
    16861 caaccttttc cagttgttga agggcctgac ttgccggatc acgggccaaa ggtagctcct
    16921 cctcaacagc cttcttggtc gatcgaactt ggtcgagctg ttgctgagtc aattgttcca
    16981 attctgctgc ccgttgccgg gcaagatttg ctcgatcggg gttctgtccc tcgctgccga
    17041 ccatggattg caccgcatca actagttccg cggccaagcg tgccctttct gcatcggcta
    17101 tcgccatttc aacagcagaa atcgcttcgt cgatagcctg ttctgcccgg cgagtagcca
    17161 tgcgaaccgc gtgatcctcg tgcgatccct ctgctttggg cggcttctcg acctgtgggc
    17221 gattctctcc agacgaggct gtcgtgtttt gatcatcctt gcccgcctct gggggatgat
    17281 ccggatgtgc ctgattggaa aattccccct ggccctcctg gccacttggc ttcgtttggg
    17341 tcttggacag ctcggatagt gcctgagccg attgtcccac ggtctcggcg gctttcctaa
    17401 tcgcctcggt gacatcctgg ggcagagatt ttttcgtcgg gacctcgttg attttctgac
    17461 tcaaccgggt ctctcccttt gcagcctcct ccagttctcc ttgccgtgcc gtctgactga
    17521 ccgtggcttg ttcttcacgg atttcctgga gttccacctt ggcctgccgc aagtcggcta
    17581 attgagaacg gacagcctcg gaatttggca ggccggtaaa ctcgcccgtc ggcgattccc
    17641 gttgaagcgt cgttagggct tctgcaaggt gatcaatgac cgattgttga tgctcagtag
    17701 cctgttgtgt ttgaccttga aacagcgttt cagtggcttg acgcgccgct tcggcagcct
    17761 ggtttaatgc ttcctgtccc tcggggagct tcttggcggc ttgttcaatc tccttttgca
    17821 actggagctg attctcggtc aaggtttgga cttcttgtgg gttagcttcc gcatttaggg
    17881 tcttttctcg caggtcggct tggcgctctg ccaaagcctc aattattcgc cgggccgcat
    17941 cgcgggaatc actttccatt tcgccctgga agcggcgaac tcgctccagg agag
//
LOCUS       DAOSUT010000080        17835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_1991_length_17835_cov_9.907143, whole genome shotgun sequence.
ACCESSION   DAOSUT010000080 DAOSUT010000000
VERSION     DAOSUT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 17835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17835
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_17835_cov_9.907143"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PLD05_08935"
     CDS             complement(<1..1047)
                     /locus_tag="PLD05_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOP77605.1"
                     /translation="MDHDILLIVRHLKKYFPKRRGLLSRVVANTRAVDDVSFAIRRGE
                     TLGLVGESGCGKTTLGRTVVRLLEPTSGTIVFDGQDVLKATGQELRAMRRRMQIVFQD
                     PFSSLNPRMTIKSIIEEGLIVHQRCRELLKANSSTESNNSSTTQTLTRAQAKQKRHER
                     VMKILEEVGLSPKFADRYPHELSGGQRQRVSIARALILRPDFIVLDEPLSALDVSIQS
                     QIINLLVALREQYRLTYLFISHDLSVVQYLSDRVAVMYLGEIVELADSLRLYAEPLHP
                     YTQALLSAIPTMEPGSGRRRIILEGDPPSPENPPPGCRFHPRCPKRMDICSREVPRIL
                     DLDGHYVCCHLVEKE"
     gene            complement(1066..2103)
                     /locus_tag="PLD05_08940"
     CDS             complement(1066..2103)
                     /locus_tag="PLD05_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP77606.1"
                     /translation="MTAQIAPTQKPLSTELQPLLRVENLCTYFFTDDGILRAVDDVSF
                     EIKRGRTLGLVGESGCGKSVTALSLMRLMAPPGKIVRGKITFWDAKNKPTVLTDLSDR
                     EMRAVRGRDIAMIFQEPMTSLNPVFTVGSQIIEAIQLHQKKSFSEAKDLTIKLLQQVG
                     IPNAEVRWRDYPHQFSGGMRQRVMIAMALSCNPALLIADEPTTALDVTIQAQILELLK
                     QIQHERQMSILIITHDLGVVAELADDVAVMYASKIVEQASVQELFEQPYHPYTVGLFR
                     SRPRLGQDKRTRLATIEGNVPNPKDFPSGCKFHPRCPYRQKICEQEEPDLREIAPCHF
                     VRCHFAGELRF"
     gene            complement(2140..3426)
                     /locus_tag="PLD05_08945"
     CDS             complement(2140..3426)
                     /locus_tag="PLD05_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77607.1"
                     /translation="MTSEQSYWDIVWRQFCKNRLSLAALWTLAPLFLLAIFAPAIASN
                     VPFYFRDEHGAIWPWFRALLNPEETVDYWFNLALVAFFPWVAVSAIAYYRLRTKYRRK
                     RVPVGAFAMLYVGMIMALAVLLSIPGVRPDNRYRARTFPEEEFHRQGQVVAIYPLIPF
                     GPTEQDMDSPVKPPLYRKPRYVPGPTGPDGQPGKPVVFWRESNDGFPHILGTDDVGRD
                     VLVRMLYGLRISLTVGFVAVGIYMAIGILLGALAGYFGGLIDIVISRLIEVIMLFPAF
                     FLILTLVGLLGQSIYIIMVVIGLTGWPTIARLIRGEVLKQRNTDYVTAAKATGASDLR
                     IIFRHVLPNAVSPAMVAAPFGIAGAIITEAGLSLLGFGVRLPAPSWGLLLRTGSENYH
                     YWWLVVFPSLAMFITVTAFNLVGNGLRDAMDPRLRI"
     gene            complement(3423..>3808)
                     /locus_tag="PLD05_08950"
     CDS             complement(3423..>3808)
                     /locus_tag="PLD05_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77608.1"
                     /translation="AGLAMYARTSMLEVISQDYIRTARAKGLSEFKVIFKHALRNALI
                     PILTLFSNFLPALLGGSVLVEYIFGIHGMGRLSWESIEQKDYPTLMALIYIDAIIVLL
                     SILLTDILYVLVDPRITFSARGEEQ"
     assembly_gap    3809..3908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3909..5035)
                     /locus_tag="PLD05_08955"
     CDS             complement(<3909..5035)
                     /locus_tag="PLD05_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77609.1"
                     /translation="MWSYILRRLLLMIPTLFGITVVSFCIMQLAPGDPLLLQLGATGL
                     AGQTTQTREAYLIQKRDLKLDRPLLINLRYFRNYRDMVYWAGYYRAADELQIAAELAA
                     LSRDPQGIADGPRKLAFLRALKIPRFDQRLQDAREHRRLARAISIYVQVFCEDLGWHG
                     VGPAIAHLQDPATDRQIRIGLIHALNSMVVDPFVFTYSSRPTDKETPYVVAAWRLWWE
                     RAEATARPLSQEDRAELEARFHALCQMSQNELLEELDYLDPETVRFYAEKLLGDASLQ
                     ERFVASLALRRLVRQPLRLDVPLNALDTVLEEVIRNWSEHYQIYRSRYEPSLAARLAY
                     IFTDTQYAHMVWRLVTFQFGRSATKTREPVATKIWNAVVVSA"
     gene            complement(5288..7054)
                     /locus_tag="PLD05_08960"
     CDS             complement(5288..7054)
                     /locus_tag="PLD05_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOP77610.1"
                     /translation="MVDAVAGSAPRSDSVEPPTTEPLPWQERLTRGGVKPAVNDVLLL
                     SYPDDPDTLNPITSSDTVSEAFQSRVYETLAEPRFENPDEWDPVLAESWDFDEEKLEF
                     TIRLRKGVKWHPMKLPNGQPLPDAELTARDVIFSFDCVMNPNVEAAHIRSYYEDPSAT
                     DEEGRRMIEYKAVDKYTVKVRWKKPYFLAKEFTLNGFAIIPRHVYSVDAEGKPISFDF
                     SSKEFADGFNNHWANKMMCGTGPMIFKEWVSQQRLVLVRNPDYWGEPYYFSGVTYRCI
                     SNSNTNMQMLLQNELDFSGIADKDLFLQAKEHPNVKAGKVCLVDYPYPRYRYIGYNLD
                     KPVFKDREFRWALAYAIPVDTIIEKVFRGLAGRTTGPFLPGSSSYDETLPPIPYDLNK
                     AREMLEKAGWVDTDQDGVRDKVIDGQKVPARFELMIYTDAPSYRQIAEIVKEECRKIG
                     IDVQIAPTKWNLMLQKLRKREYEAAMLGWAMSWKQDPFQLWHSSQADAPDSSNHVAYR
                     NPEVDRLIEELRVTMNEEKQHELYKKIHRLIYEDQPYTFLFSDRATGGYDARIQNVKF
                     YKIRPCYDGREWYSNRPRAVGP"
     gene            7572..8654
                     /gene="sppA"
                     /locus_tag="PLD05_08965"
     CDS             7572..8654
                     /gene="sppA"
                     /locus_tag="PLD05_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOP77611.1"
                     /translation="MSHPSSGEGIIQAEVLAVEPAHRKSVFWRVLKVLIGLLVIVTLF
                     VSLLLNMFFIGTLGLDGMGSKKVREKYFSHSRFARDKVVIMPLEGLILDGDGFMKRQI
                     DQVIEDETVKAVVLRVNSPGGTVSGSHAIYHYLKKMCEKRKIPLVVSMGAIAASGGYY
                     VSMAVGDTPKSIYAEPTTWTGSIGVIIPHYDAAELAQKIGIKEDSVASHPLKGMGSML
                     RPMTEEERAIFQGLVDESFNRFKEVVKSGRPKFRQQPELLDKLATGQVFPAEQAKAEG
                     LVDEIGFLEDAVDRAIELANLDPDNVRVVKYEKELTLMDVLLGETVQVHIGPAGMDWK
                     SLLEWSVPRAYYLFYQGPVLIQSGLR"
     gene            complement(8646..9572)
                     /locus_tag="PLD05_08970"
     CDS             complement(8646..9572)
                     /locus_tag="PLD05_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77612.1"
                     /translation="MARILVVDDSPLDRQWLAGLLATIPDAEIDEAADGEEALRIVLE
                     RQPDIVVTDMQMPKLDGLQLVTAIRRGSPHIPVILVTGHGSEESALQALRVGAASYVN
                     KEWIPQRLIPTVKQVLEAAQHRAMISRVSRFLTGCHFGFVLENDCQLLRPVLEFVHDS
                     LECFDLDLTEITRVNVALQEALSNAIFHGNLELSSRLREEDESRFFQLARERRQRPPY
                     RDRRVFLEGELTREKISFAIRDEGPGFDPSLLPNPHRTEALDCACGRGLLLIRSFMDE
                     VSFNARGNEIRLVKYLRREDPSQEHLSPTGLS"
     gene            complement(9718..13494)
                     /locus_tag="PLD05_08975"
     CDS             complement(9718..13494)
                     /locus_tag="PLD05_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77613.1"
                     /translation="MSHNGVNDRSVESPPTAVSWWARARVWIATLVVLAIALAVSGYI
                     WRVTAQRETMRIVSQCSHQSQLQVQNIEFKLQQSQNLLQFLRSFFAASEDVHRYEFAE
                     FCRSHLDNIPVVKFAAWAPVTYPDGFETLAECADRHGVANFQPWELDEAGQRTFVQTR
                     PQHFPILFIEPSPPFDRLVGLDIASIPSWAEALEETIMIGEVDVHFLEGVPAIDEVVF
                     EADSGVGSSSLLESEEETFSTQYSRSSAAGLAPRKYLAICSPVFFGGYIPENPFERLT
                     QCGGVLVCVVDVQELLRPLKRWAAVEGINFRLVDLQGTSQHVVEAWERPSSRTERLLQ
                     TYPTSSWDIRLPGRVWRVITQPTATYVASRRGSGSWGVLAAGTLCSFLLALYVNVIGG
                     RSLRIQQLVAEKTHELRETNRRLEAEIEQREKVEKGLRDSEALYSSLVESLPLHVLRK
                     DREGHFTFANTAFCHLVGRSREEIYGKTDFDFYPPDLASKYRRDDRRVMETGEIFEDI
                     EEYEKDGEIRYVQVLKSPVRDHAGNIVGVQVAFWDVTEKVAVQRALEKAKQAAEEANR
                     AKSDFLANMSHEIRTPMNAILGMAQLLEQTPLNAEQKEYLRIIREAGDNLLALINSVL
                     DFSKIESGRIELDSVDFNARELIGDTMKALAVRAHKKQLELACRVAPNVPEELVGDPV
                     RLRQILINLVDNAIKFTEQGEVVVDVTCDECTTSDVVLRCSVRDTGIGIPPEKQQVIF
                     AAFEQADKSTTRKYGGTGLGLAICKRLVNLMGGTITVESQPGVGSTFTFTARFRRPSE
                     TLPKGLPVDLREYAHRPILVVDDNATNRVILSEMIANWGLKSELAASGEEALRLVTDE
                     AGQVRFPVVITDMRMPGMDGMALIRQLRRRFGAEQLAIIVLSSEDRPTDSEFFRRFEV
                     SGVLRKPVKQSELYNILAEILGLAFHPESKTTIPASFPELPPLQILLVEDSPFNQQVA
                     IGVLEKRGHTVTVASNGKEALEILRNRSFDLILMDIQMPEMDGLTATKEIRAREERLQ
                     QPRTPIIAMTAHALKSDREAALKAGMDGYVAKPIVVEEFFQTIADVLHQVRGINLGDT
                     QALPTLEMSTSSPDGSISPTPTAADTPAPQENSLPSPPTSEVNSPESALETRPGESTH
                     VDWQHALRAAGGDRELLRALVNTFIQEMPRQWENLEKAVSQEDWPTARRATHTLGGSF
                     RHFGARRAADVAYQAEQALAQGGSQRDLTEPLQQLYHCLQEILRELREARDRGI"
     gene            13913..>17835
                     /gene="hrpA"
                     /locus_tag="PLD05_08980"
     CDS             13913..>17835
                     /gene="hrpA"
                     /locus_tag="PLD05_08980"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOP77614.1"
                     /translation="MLLTGRELSRLERQIDQAMRADRFRLGRWLRRLCTRARDRRLSD
                     EEVRRFEEELEASVTLRRKRLTERPYVEILPNLPIFAKRDEIAEAIRANPVVIVCGET
                     GSGKSTQLPKICLQIGRGIEGLIGHTQPRRIAARSIATRIAQELGVTLGREVGYKVRF
                     SDVTSAETYIKLMTDGVLLAETQSDRFLEHYDTIIIDEAHERSLNIDFLLGYLHQLLR
                     RRRDLKVIITSATLDAERFAQHFALGQRPAPIIEVPGRSYPVEVRYRPPLPDESTGEI
                     DWQSAVVAAMEEVLAEGPGDILIFMPTERHIHETMRLLKARASGDPRFRDIELLPLYA
                     RLSATEQQRIFQPHKQRRVVIATNVAESSLTVPGIKYVIDPGVARLSRYSPRTKTQRL
                     PIEPISQASAEQRKGRCGREGPGLCIRLFSEEDLLVRDRYTPPEILRTNLASVVLQLM
                     ALNLGQVETFPFLDAPRADAIRDGYKTLFEIGAVDEEGKLTPLGRRLAKLPVDPRIGR
                     MILAAHEEGCLHEVLIIAAALEVDDPRLRPAERAEEADLAHAAFRHPDSDFLGYLKLW
                     DFYQNLKEKLSRNQLQKACQQNFLSYNRLREWADIFRELQEIVTESGMKISKRRNDAA
                     AIHRAILTGLLSNVAMRHNRYEYTVASGGKAYLWPGSGVFQSKPPWVVAAELVETGRR
                     YLRICAKIDPAWVEVLAPHLIKKSYVDPYWSRRYGAAMVYEKVTLFGLPVVPRRRTPL
                     GPVDPQTAREMLIQHGLVEGEIHCDAAFFVHNQRLVREMETAQVKLRRVDLLLGQWAQ
                     FDFYDQRIPSHVYDLDGLLHWWNEASKSDPNILHMRPSDVVREEPPPDIDKQFPDHLL
                     VEKAPLPLTYCFQPGQPNDGVTVTVPLELFHCLDPRRLEWLVPGMLESKIAALIKSLP
                     KEKRRRLIPVQETARQTLQMLSFGQGRFLPAVAEVLSRLAGETIRPEDFQLDKIPPEL
                     HMSIQVVDAEGKVVAKGRNWTELREKLGTRQAVAFSAIDDPHWNRDGLTDWDFDLLPE
                     EIEVRRGDIPVKAFPMLVDAGNTVSLRLADSPARAAHQSRFGIRRLLAIAVLPHLDPR
                     WDEFPDCDRLRLMAATLPGFDFQNQLLLALIDRAFLDESLVGPWQIGEWGNLPRTRAE
                     YRRLFRMGLKRLPLAVQEVLSLIRPLLETYHQAAIALQNADSVQWEESRADIQEQLAE
                     LTCPGFLTHTPWNWLRQYPRYFRGISRRIEALRLGGVFRDREAMAVFRPYWEAFLQRR
                     QFHQELDIFDPELVLYRWMLEEFRISLFAQALGTAIPVSPQRLDR"
BASE COUNT         3916 a         4884 c         5044 g         3891 t
ORIGIN      
        1 ctctttttcc accaagtgac agcagacata atgaccgtcg aggtcgagaa tccgcgggac
       61 ttcccggcta cagatatcca ttcgcttggg gcagcgcggg tgaaagcggc aaccgggcgg
      121 cgggttctcg gggctggggg gatcaccctc caggatgatc cggcggcgac cgctgccagg
      181 ctccatcgtg ggaattgccg agagaagggc ttgcgtgtaa ggatggagcg gctcggcata
      241 gagtcggagg ctgtccgcta gttcgacgat ctcgccaaga tacatgacgg ccacgcgatc
      301 ggacagatac tgcaccacgg acaaatcatg agaaatgaaa agataagtca ggcgatattg
      361 ctcgcggagc gcgaccaaaa ggttgatgat ctgcgattga atcgacacat ccagggccga
      421 aagcggttca tcaaggacga tgaagtcagg tcggaggatc agggcgcggg caatactcac
      481 gcgttgtcgc tgtcccccgg aaagctcgtg gggatagcgg tcggcaaact tggggctgag
      541 gccgacctct tcaagaatct tcatgacgcg ttcgtgacgc ttttgtttgg cctgggcccg
      601 ggtcagagtt tgtgtcgtag aggaattgtt cgactcggtg gaggaatttg ccttaaggag
      661 ttcccggcat cgctggtgga cgatcaggcc ttcttcgatg atcgacttaa tcgtcattcg
      721 gggattgagc gagctaaagg ggtcctgaaa gacgatttgc atccttcgcc gcattgcccg
      781 aagttcctgg ccggtcgctt taaggacgtc ctgcccgtcg aagacgatag ttccgctggt
      841 gggttccaac aatctaacga cagtgcggcc caaggttgtc tttccgcagc ctgattcgcc
      901 caccaggcca agcgtttcgc cccggcgaat ggcgaaggag acatcatcca cggcgcgggt
      961 attggccacc acgcgcgaga gcaaaccccg gcgcttgggg aaatattttt tcaggtgtcg
     1021 aacaatcaat aagatgtcgt gatccatagg ccttaatttt gttgatcaaa aacgcagttc
     1081 gccggcgaag tgacagcgga cgaaatggca aggtgcgatt tcacgcaagt ccggctcctc
     1141 ttgttcacaa atcttctggc gatagggaca ccgcggatgg aacttgcagc cgctcgggaa
     1201 gtctttggga ttggggacgt ttccttcgat ggtggcaagc cgtgttcgct tgtcctgccc
     1261 taaacggggc cgggaacgga ataaacctac cgtgtaggga tgatacggct gttcaaaaag
     1321 ctcttgcacc gacgcctgtt cgacgatttt ggatgcgtac atgacggcca catcgtcggc
     1381 caattcggct accacgccga gatcatgggt gatgatcaag atggacattt gccgttcgtg
     1441 ctggatctgc tttagcagtt ccaggatttg cgcctggatg gtgacatcga gggccgtcgt
     1501 aggttcgtcg gcgattaaga gggcggggtt acaggatagg gccatggcga tcatgacccg
     1561 ctgtcgcatc ccgccagaga actgatgggg ataatctcgc caacggactt cggcattggg
     1621 aatgcccacc tgttggagca gcttgatcgt caaatcctta gcttcgctga aggacttctt
     1681 ttgatggagt tgaattgcct cgatgatttg cgagccgacc gtgaagacgg gattgagtga
     1741 ggtcatcggt tcctgaaaga tcatggcgat atcacgaccg cgaacagccc gcatctcgcg
     1801 atcgctgaga tcggttagga cggtgggctt gtttttggcg tcccagaatg tgatttttcc
     1861 tcgcacaatt tttccgggag gggccatcag ccgcatgagt gacaaggccg tcacggactt
     1921 cccgcagcca gattccccca ccaagcccag ggttcggcct cgtttgattt cgaaggaaac
     1981 gtcgtccacc gcccggagaa taccgtcgtc ggtgaaaaaa tacgtgcaaa ggttttccac
     2041 ccgcagcagc ggttgcaatt cggttgaaag gggcttttga gttggggcga tttgggccgt
     2101 cataagtggg cttcagtgat cacgatgatt ggatctcgtc tagattcgta ggcgtgggtc
     2161 catggcgtca cgcaggccgt tgcccaccag gttaaaagcc gtcaccgtaa taaacatggc
     2221 cagtgaggga aacaccacca gccaccagta gtgatagttt tcacttcccg ttctaaggag
     2281 caacccccag cttggtgcag gaagacggac gccgaaacca aggaggctca gcccggcctc
     2341 ggtgatgatg gccccggcaa taccaaacgg ggccgcaacc atagctgggg aaaccgcgtt
     2401 gggcaaaacg tgtcgaaaga tgattcgcag atcggaggca cctgttgcct tggccgccgt
     2461 cacgtaatcg gtgtttcgtt gtttgagcac ttcaccgcgg ataagtcggg cgatggtcgg
     2521 ccagcctgtt aggccgatca ccaccatgat gatgtaaata ctttgcccca ggagtcccac
     2581 caacgtcaaa atgagaaaga atgccggaaa aagcataatg acctcgatca atcgcgagat
     2641 aacaatgtcg atcaatccgc cgaaatagcc ggccagggcg cccagcagaa tgccaatcgc
     2701 catgtagatt cccacagcga caaatccgac ggtgagcgag atacgtaggc cgtacaacat
     2761 ccgcacaagc acatcccgtc cgacgtcgtc tgtccccaag atgtgcggaa agccgtcgtt
     2821 gctctctcgc cagaagacga ccggtttgcc aggttgcccg tcgggacccg tcggaccggg
     2881 cacatagcgc ggtttgcgat acaagggcgg tttaacaggg gagtccatat cctgttcggt
     2941 tggcccaaag ggaatcagcg gataaatggc taccacctga ccttggcggt ggaactcttc
     3001 ttcggggaag gtccgtgccc gatagcggtt gtcgggccga accccaggga tggataacaa
     3061 caccgccaga gccatgatca tccccacata taacatggca aaggccccca cgggtacgcg
     3121 tttgcgacgg tattttgtcc tgagccggta ataggcaatt gcggaaacag caacccaggg
     3181 gaaaaaggct accaaggcaa gattgaacca atagtcaacg gtctcttcgg ggttcaagag
     3241 ggcacgaaac cagggccaga tcgcaccgtg ttcgtctcgg aagtagaacg gtacattcga
     3301 agcaatcgcc ggggcgaaaa tggcaagcaa gaacagtggg gcaagcgtcc agagagcggc
     3361 taacgagagc cgattcttgc agaactgtcg ccacacaatg tcccagtacg actgttccga
     3421 cgtcattgct cctcgcctcg cgcggagaat gtgatccgcg gatcgaccaa cacgtacagg
     3481 atatctgtca gaaggatact caacaaaaca atgatggcgt cgatgtagat taaggccatc
     3541 agcgtgggat agtccttttg ctcgatggat tcccagctca agcggcccat gccgtggatg
     3601 ccgaaaatgt attccaccaa gacacttccg cccaaaaggg ccggaaggaa gttggaaaac
     3661 agcgtaagaa tggggatgag ggcatttcgc agggcgtgct tgaagatgac tttgaactcc
     3721 gaaaggccct ttgcccgagc cgtgcgaatg taatcctggg agatcacttc gagcatgctt
     3781 gtgcgggcat acatggccaa tcctgccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnngc ggagacaacg acggcgttcc aaatcttggt agccaccggt tcccgtgtct
     3961 tggtggccga gcggccaaat tggaacgtaa ctaaccgcca caccatgtgg gcatactgtg
     4021 tgtcggtgaa gatgtaggcc aaacgtgcgg caagactggg ttcgtaacgg ctccgataga
     4081 tctggtagtg ctcggaccag ttgcggatga cttcctccag gacggtatcg agggcattga
     4141 ggggcacatc aagccgcaag ggctgtctga ccagtcgtcg tagggccagg cttgcaacaa
     4201 agcgttcttg gaggctggcg tcgccgagaa gtttttccgc ataaaaccgc acggtctcag
     4261 gatctaggta atccaactcc tcgaggagtt cgttctgaga catctggcac agggcgtgga
     4321 aacgtgcctc gagttctgcc cgatcttcct gggagagagg gcgagccgtc gcttccgcac
     4381 gttcccacca caggcgccag gccgccacca cgtagggcgt ttctttgtcg gtgggacggc
     4441 tggaataagt aaagacaaag ggatcgacga ccatggaatt gagggcgtga atcaggccga
     4501 ttcgaatctg gcggtccgtt gcggggtctt gcaggtgagc aattgccggc cccacgccgt
     4561 gccagccgag gtcttcgcag aagacttgca cataaatgct aatggcgcgg gccaaacgcc
     4621 gatgttcacg tgcgtcttgt agtcgctggt caaagcgggg aattttgagc gcccgcaaaa
     4681 aagccaactt acgagggccg tcggcgattc cctgcggatc ccgagaaagg gcggccaact
     4741 cggcggcaat ttgaagttca tcggccgcac ggtaatagcc tgcccaatac accatgtcac
     4801 gataattacg gaaataacgc aggttgatga gaagcgggcg atccagcttc aggtcgcgct
     4861 tctggatgag ataggcttcg cgggtctggg tggtctggcc tgccagtccg gtagcaccaa
     4921 gctgcaagag gaggggatca cccggcgcca actgcatgat gcagaaggac accaccgtga
     4981 ttccgaacag cgtcggtatc atcaacaaga gacggcgaag gatgtaactc cacacggatc
     5041 gttcgttccg tcttcagtca gtgactaagg ttcattgctt gtcgcttatg cttctcggtt
     5101 atacgccatt tcgctttccc gcgaggtggg aagattacgt cagaagccct ttccccggca
     5161 agatcgcgtt atcacggcga cgtccgttcc atcgtcccga ccggtactcg aaaattggac
     5221 catcccgttt gtcttcggaa acccgcggcg gacataacaa aacatgaccc ttcctgtacg
     5281 ggcacactca cggcccaaca gcccgcggcc ggttggagta ccattcgcga ccgtcgtagc
     5341 agggtcggat tttgtagaac ttcacatttt ggatgcgtgc atcgtatccg ccggttgccc
     5401 gatctgagaa caaaaacgta tagggctgat cttcataaat cagccgatga atcttcttat
     5461 acaattcgtg ctgtttttcc tcgttcatcg tgacgcgcag ctcttcgatc aaacgatcaa
     5521 cctcggggtt gcgataggcc acatgatttg aactgtcggg cgcatccgcc tggctggaat
     5581 gccacagttg gaagggatcc tgtttccacg acatggccca cccgagcatg gcggcctcat
     5641 actcccgctt gcggagtttc tggagcatca ggttccattt cgtgggggca atctgaacat
     5701 caatcccaat cttgcggcac tcttccttca cgatctccgc gatctggcga tagctgggag
     5761 catcggtata aatcatgagc tcgaaacggg cagggacctt ttggccatca atcaccttgt
     5821 cccgcacacc gtcttgatcg gtgtcaaccc aaccggcctt ttccagcatc tcccgggcct
     5881 tgttcaaatc gtacgggatg ggcgggaggg tctcatcgta ggagctactc cccggaagga
     5941 atggtcccgt tgtccgaccg gccaatcccc gaaacacctt ctcgataatg gtatcaacgg
     6001 ggatggcata ggccaaagcc cagcgaaact cccggtcctt aaacacgggt ttgtcaaggt
     6061 tgtagccaat gtaccgataa cggggatatg gatagtcaac cagacaaacc ttgccagcct
     6121 tcacgttggg atgttccttg gcttggagga agaggtcctt gtcggcgatg cccgaaaagt
     6181 caagttcatt ctgaagaagc atctgcatgt tggtgttgga gttggaaatg caccggtatg
     6241 tcactccgct aaagtagtac ggctcacccc agtaatccgg atttcgcacg agcactagtc
     6301 gttgttgcga cacccattcc ttgaagatca tgggaccggt gccgcacatc atcttgttgg
     6361 cccagtgatt gttgaagccg tcggcaaact ctttggacga gaaatcgaac gagataggct
     6421 ttccctcggc atccaccgag tacacgtgcc gagggatgat ggcgaatccg ttcagggtaa
     6481 actctttggc cagaaaatac ggctttttcc agcggacttt gacggtgtat ttgtccacgg
     6541 ccttgtattc aatcatccgg cggccctctt cgtccgtagc cgagggatcc tcgtaatagc
     6601 tgcggatatg ggcagcttca acgttgggat tcataacaca gtcaaagctg aagatcacat
     6661 cgcgggccgt cagctcggca tcggggagcg gttgaccatt ggggagcttc atcggatgcc
     6721 atttgacgcc ttttcgcaaa cggatcgtga actccagttt ctcctcatca aaatcccaac
     6781 tttccgccaa aacagggtcc cactcatcgg gattctcaaa ccttggctcg gcaagggtct
     6841 cgtacacccg cgattgaaag gcctcggata ccgtgtccga tgaggtaatt gggttgaggg
     6901 tatccggatc gtcgggataa ctcaaaagga gcacgtcatt aacggcgggt ttgactcccc
     6961 ctcgagtcaa tcgttcttgc catggaagag gttcggtagt cggtggttca accgaatcgc
     7021 tgcggggtgc ggaaccggcg accgcatcaa ccatgcttgt gtccgatttc gtgcaacccg
     7081 aaaaagcggc taagatcact aaaatcggcg cgactttcac taatgacagc ctctccatat
     7141 attactccct ttccacgcag ccgctggatc acgggatgga gttaactgaa ctcgacaaaa
     7201 tgagtcctgt aagctccgtc agcgacgggc gaccattcaa agtcattacc acagcgaacg
     7261 ggaacggtat caccctcccg ttgggattcg attcctggta tctgaaacaa ggtgcagcca
     7321 gattaaaact ccaccgtttg tggcatgtta agatattggg atgatgcggt caataacgac
     7381 cggcgaccgt tcggcgagtt ggtgagttgg ttaatcctgc aaagcaacgt aaaataacgc
     7441 aggttctgat tcaacggtgg gaaagcgggt atttttcaca tcacggcggc acggcggtag
     7501 ccgtacgtgt gtggcgtcat cggcagttct ccgaagtgtc ggaccttttt acttaaatgt
     7561 tggagcagca tatgagtcac ccatcttctg gagaaggcat tatccaagcg gaagtgttgg
     7621 ccgttgagcc ggctcaccgg aaatctgtct tctggcgcgt tctcaaggtc ttgatcggct
     7681 tgctcgtgat cgtgacccta tttgtgtccc tgttgctcaa catgttcttt atagggacgt
     7741 tagggctgga tggcatgggc agtaagaaag ttcgggagaa atatttttcc cactcccgat
     7801 ttgcccggga taaggtcgtt atcatgccgc tggaaggctt gatcctggat ggggacggct
     7861 ttatgaagcg gcaaattgac caggtgatcg aagacgaaac ggtcaaggcc gttgtccttc
     7921 gggtgaactc cccgggggga accgtgtcgg gatcgcatgc catttatcac tacctcaaaa
     7981 agatgtgtga aaaacgaaaa atccccttgg tggtcagtat gggggcaatc gctgccagtg
     8041 ggggctacta tgtgtcgatg gccgtcggcg atacgccaaa atccatttat gccgagccca
     8101 caacgtggac agggtccatc ggtgtgatca tcccccatta tgacgcggca gaattggcac
     8161 agaaaatcgg tatcaaagaa gattctgtgg ccagccaccc cttaaaaggg atgggcagca
     8221 tgctacgtcc gatgacagaa gaggagcgag ccatctttca gggcctggtg gatgagagct
     8281 ttaaccgatt taaggaagtc gtaaaaagtg gtcggccaaa attccggcag cagccggaac
     8341 tgcttgataa attggcaacc ggtcaggtgt ttcccgccga gcaggccaag gcggaggggc
     8401 tcgtggacga gatcggcttc ttagaggatg ccgtcgatcg ggcgatcgaa ttagccaatt
     8461 tggatcctga caatgttcgc gtcgttaagt acgagaaaga attgacgtta atggatgtgc
     8521 tcttgggcga gacggtccag gttcatattg ggccggccgg catggattgg aagtctctcc
     8581 tggagtggag tgtcccccgg gcctattacc tcttttatca aggcccggtc ttaatccaga
     8641 gtggtttacg ataagccggt cggggagaga tgctcttgcg agggatcttc ccgccgcaaa
     8701 tatttaacca agcgaatttc gtttccccgg gcgttgaagc tcacctcgtc cataaatgat
     8761 cgtatgagaa gcagcccccg gccacaggcg cagtcgagcg cctctgtgcg atgcgggttg
     8821 gggagcaaag aaggatcgaa gcccggtcct tcgtcgcgga ttgcaaaaga aatcttctcg
     8881 cgcgtcagtt ccccttccag aaagacgcga cgatcccggt aaggtggccg ctgacgacgt
     8941 tcccttgcca actgaaagaa acgggactca tcctcctccc gtagccgcga ggaaagttcc
     9001 aagttgccgt ggaagatagc gttggacagg gcctcttgaa gcgctacgtt gacgcgcgtg
     9061 atctcggtca ggtcaaggtc aaaacactcc agtgaatcgt gaacgaattc taagacgggc
     9121 ctaagcagtt ggcagtcatt ttccaggaca aagccaaagt ggcaacccgt caggaatctc
     9181 gacacccgcg agatcatggc gcggtgctgg gcggcctcca gaacctgctt gacagtggga
     9241 ataagtcgct ggggtatcca ctctttgttg acgtaacttg cggcgccaac gcgcaatgct
     9301 tggagtgccg attcctcgct gccatggccc gtgacaagga tcacgggaat atggggggac
     9361 cctcgccgaa tggccgtgac cagttgcagc ccatcgagct tgggcatttg catatctgtc
     9421 acaacgatgt caggttgcct ctccaggacg atgcggaggg cctcctcgcc gtcggctgct
     9481 tcatcaatct cagcgtcggg aatcgtggcc agtagccccg ccaaccactg acggtcaaga
     9541 ggggaatcat cgaccactag aatccttgcc atgtgcggat ccttcgcaag cgttcacgat
     9601 ctcagggcac ggtcatgggc gacacattct tggacaacgc tcttcttttt cctggaagac
     9661 ccaactttct tgccttatat cttccccttt ggggagaaat ctttggacat cagcatttta
     9721 gatgccgcga tcacgggctt ctcgcaattc ccggaggatt tcctgcaaac aatgatacaa
     9781 ctgttgtaaa ggttctgtca ggtcacgctg tgagccgccc tgggcaaggg cttgctccgc
     9841 ttggtacgcc acgtcggcag cacggcgggc gccaaagtgc cggaacgatc cgcccaaggt
     9901 gtgggttgcg cgtcgtgctg tgggccagtc ttcttgagaa acggcttttt ccagattttc
     9961 ccactgtcgg ggcatttcct ggatgaacgt attgaccagg gcacgcagca actcccgatc
    10021 tcctcctgcc gcccgcaagg cgtgttgcca gtccacatga gtgctctcgc cgggccttgt
    10081 ttctaaggcg ctctccggcg agttcacttc gctggttggt ggcgatggca gcgaattctc
    10141 ttgaggagcc ggtgtatcgg ccgcagttgg tgtgggggaa attgaaccgt ctggagatga
    10201 cgtgctcatt tcgagcgttg gcagagcctg cgtgtcaccc aggttgatgc cacggacctg
    10261 gtgcaagaca tccgctattg tttggaagaa ttcctccacc acgatgggct tggctacata
    10321 gccatccatg ccggctttca aggcagcctc ccgatcgctt tttagggcgt gggccgtcat
    10381 ggcaataata ggagtccgcg gttgttggag gcgttcttcc ctggcccgaa tctccttggt
    10441 tgccgtcagg ccgtccatct ccggcatttg aatgtccatc aggatgaggt cgaaagaccg
    10501 gttgcggagg atttccaaag cctctttgcc attggaggcg acggtcaccg tatgcccacg
    10561 cttttccaac accccgatgg ccacctgttg attgaaagga ctgtcctcca cgagcaaaat
    10621 ctgcaagggg ggaagttctg gaaaactggc cgggatggta gtctttgact cgggatggaa
    10681 tgccagaccc aggatttcgg ctaggatgtt gtaaagctcc gactgtttga ccggcttgcg
    10741 gagaacaccg gaaacctcga agcgtcggaa aaactcgctg tcggtcggtc ggtcttccga
    10801 ggaaagcacg atgatcgcca gttgttcggc gccaaaacga cgtcgcaact ggcggatcag
    10861 cgccataccg tccatgcccg gcatccgcat gtcggtaatg acgacaggaa agcggacctg
    10921 tcctgcctca tccgtgacga gccgtaaggc ttcttcccca gaggccgcca attccgactt
    10981 caatccccag ttggcaatca tctcactgag gatgacccga ttggttgcgt tgtcatcgac
    11041 gacgaggatg ggtcggtggg cgtattcccg caaatcgact ggtaagccct tgggaagtgt
    11101 ttcgctgggg cggcggaatc gggcggtgaa ggtaaacgtg ctgcccaccc ctggctggct
    11161 ctccaccgta atggtgcccc ccatcaagtt gacaaggcgt ttgcagatcg ccagccccag
    11221 gcctgtcccc ccatattttc gcgtcgtgct cttgtccgcc tgctcgaaag cggcaaatat
    11281 cacctgctgc ttttccgggg gaataccaat gcctgtgtcg cggacggagc agcggaggac
    11341 aacgtcgcta gtggtacatt catcgcacgt gacatccacg acgacttcgc cctgctccgt
    11401 gaattttatg gcattatcga caagattgat aaggatttgt cttagccgaa cgggatcgcc
    11461 gaccagctct tcggggacat ttggggcgac gcggcaggcc agctcgagct gtttcttgtg
    11521 ggcccgcaca gccagggcct tcatcgtgtc accgatcaac tcgcgggcgt taaaatccac
    11581 gctatccagt tcaatgcgtc ccgattcgat cttcgagaaa tcaaggacgc tgttgatgag
    11641 cgcgagtaga ttgtcaccag cctcacggat gatgcgaaga tattcctttt gttcggcgtt
    11701 aaggggtgtt tgctcaagga gttgtgccat gcccaggatg gcgttcatcg gcgtgcgaat
    11761 ctcgtggctc atgttagcca ggaaatcgct cttggcccga ttggcctctt ctgccgcttg
    11821 cttggccttt tccaaggctc tttggacggc aaccttttcg gtcacatccc aaaacgccac
    11881 ttgcacccca acaatgttcc ccgcatgatc ccgtacgggc gatttcaaga cctggacgta
    11941 ccggatttca ccatcctttt cgtactcttc gatgtcctca aaaatctcgc cggtttccat
    12001 gacacggcga tcgtcccggc ggtatttcga ggccaaatcg ggtgggtaaa aatcaaagtc
    12061 cgtcttgccg tagatctcct ctcgcgacct tcccacaaga tgacagaatg cagtgttggc
    12121 aaacgtaaag tgcccctcac gatccttgcg caagacgtga agcgggaggc tttccacaag
    12181 agacgagtac aaggcctcgg agtcgcgcaa gcctttttcc actttctccc gctgctcaat
    12241 ctcggcctcc aatcgccggt tcgtttcccg tagttcgtga gtcttctccg ccaccaactg
    12301 ttgaatacgg agcgagcgac caccaatgac gttgacgtaa agggccaaca aaaacgagca
    12361 taacgtgccc gcggccaaaa caccccaact tcctgatcct cggcgcgaag caacgtaagt
    12421 cgcggtcggt tgggtaatca cccgccagac gcgaccgggc aaccgaatat cccaactcga
    12481 cgtgggatac gtttgaagca agcgctccgt tcgggaggac ggcctttccc aagcctccac
    12541 cacatgttgc gaggttcctt ggaggtcaac caagcgaaag ttaattccct cgaccgctgc
    12601 ccagcgtttc aggggacgca gcaactcctg gacatcaaca acacacacaa ggacaccgcc
    12661 acattgggtg agccgttcaa acgggttctc cgggatatat ccgccgaaaa aaaccggtga
    12721 acagatggcg aggtattttc gtggggcaag gcctgccgcc gaacttcggc tgtactgggt
    12781 ggaaaacgtt tcctcttcgg attcgagaag acttgaggaa ccaactcctg aatcggcttc
    12841 aaaaacgacc tcatcgatgg cggggacacc ttccaaaaaa tggacgtcca cctcgccgat
    12901 cataattgtc tcctccagag cctccgccca ggaaggaatt gatgcgatgt ctagccccac
    12961 aaggcgatca aatgggggtg acggttcgat gaaaaggatc gggaaatgct ggggtcgcgt
    13021 ttggacaaag gtcctttgcc ccgcttcatc aagttcccag ggttgaaagt tcgcaacgcc
    13081 gtggcgatct gcacactctg ccagcgtttc aaatccatcg ggataagtca ccggcgccca
    13141 ggcggcgaac ttcaccacgg gaatattatc caagtgagac cgacagaact cggcaaactc
    13201 gtagcggtgg acatcttccg aagccgcgaa aaaggatcgc aaaaactgca ataggttctg
    13261 gctttgctga agtttgaact cgatgttctg gacctgtaac tgcgattgat gactacactg
    13321 gctgacaatc cgcatggttt cccgttgggc tgtcacccgc cagatgtagc ccgacacggc
    13381 cagcgctatc gccagcacga ccaacgtggc aatccagacc cgcgcccttg cccaccatga
    13441 aacggccgta ggcggacttt cgaccgatct gtcattgaca ccgttatgag acatcagaca
    13501 agctcaatgg cgacgtagcg ccacaatgta ggcccagcca gccttcgccg cggtctttaa
    13561 aggacgtcct ccctactcct tcccagcacg cactcttgat ttcctaaggt cccctcgggt
    13621 tgaaaccatg accggtccgc aaccgacagg acgcagctag caaactcctt tgacatttta
    13681 tccaaaaaat ttctcaatag gcaatgatag acaacgtcat gccgcacgtt tattcgaaat
    13741 gctcatcatt tcccttatcg gccatgtatt tccctttaac gaatgaaccg atacagtttg
    13801 aaaaaagggc tttcgacaat tggccatcat ggcgccgaat gacgccagag cgaaagatgc
    13861 ccagactttt acgttggtac ggagccgaca gtcaataaaa gggagcgaaa ggttgttgtt
    13921 gaccggccgc gagttaagcc gactggaacg tcaaatcgat caggcaatgc gtgccgaccg
    13981 tttccgcttg ggacggtggc tgcgtcgcct ttgtacgcgt gcgcgcgatc gtcggctttc
    14041 cgatgaggaa gtgcggcgat tcgaggaaga actcgaggcc tcggtcaccc tccgccgaaa
    14101 acgactcacc gagcggcctt atgtcgaaat cctccccaat ttgccgattt ttgcaaaacg
    14161 tgatgagatt gccgaggcga ttcgggcaaa tccggtggtc atcgtctgtg gcgaaaccgg
    14221 ctccggtaaa tccacccagt tgccaaagat ctgcctccaa attgggcgtg ggattgaagg
    14281 cttgatcggc cacacacagc cccggcgaat tgccgcccga tccatcgcca cccgtatcgc
    14341 ccaggagctt ggtgtcacgc taggccggga ggtcggctac aaggtccgtt tttctgacgt
    14401 cacctcggcc gaaacctaca tcaaactaat gacggacggg gtcttgctgg cggagaccca
    14461 gagtgaccgt tttctcgaac attatgacac gatcatcatt gacgaagccc atgagcgatc
    14521 gctcaatatc gactttctcc tgggctattt gcatcagttg ctccggcggc gtcgagattt
    14581 aaaggtcatt atcacgtcgg ccacactgga tgccgagcgc tttgcccagc atttcgccct
    14641 gggacagcga cctgctccga ttatcgaggt gcctggtcgg tcttatccgg tggaggttcg
    14701 ttatcgcccg ccacttcccg acgaatccac aggggaaatc gactggcaat ctgccgtggt
    14761 ggcagccatg gaggaggtcc tcgctgaagg gcctggtgac atcctcatct tcatgcccac
    14821 ggagcggcac atccacgaaa caatgcgtct tttgaaagcc cgggcgagcg gcgatccgcg
    14881 gttccgagat atcgagctac tgccgctcta cgcgcgtctt tcggccacgg aacagcagcg
    14941 gatctttcag ccccacaaac aacggcgggt tgtcatcgcg accaacgtag cggaatcctc
    15001 tctcacggtc cccgggatca agtacgtcat tgaccccgga gttgcccggc tgagtcggta
    15061 ttctcctcgc accaagaccc aacgtttgcc gatcgagccg atctcacagg cctcggcgga
    15121 gcagcgaaaa ggccgttgcg gtcgggaggg gccggggctt tgtatccgcc ttttcagcga
    15181 agaggacctg ttggtccgcg accgctacac cccgccggaa attctccgaa ccaacctcgc
    15241 atcggtcgtc ctgcaactga tggccttgaa tcttggccaa gtcgagacct ttcccttttt
    15301 ggatgccccc cgcgcggatg ccatccgcga tgggtacaaa acgctttttg aaatcggggc
    15361 cgttgacgag gaaggaaaac tgacaccgct aggacgccgg ttggccaagc ttcctgtaga
    15421 tcctcgcatc gggcggatga ttttagccgc acacgaggaa gggtgtcttc acgaggtctt
    15481 gatcattgcg gcagccctgg aagttgatga cccacggttg cggcccgcgg aaagggctga
    15541 ggaggccgac ctggctcacg ccgcgttccg ccatccggac tcggatttcc tcggttatct
    15601 gaaactgtgg gatttctatc aaaacctgaa ggaaaagctg tcgcgcaacc agcttcagaa
    15661 ggcgtgccaa cagaactttt tgtcgtataa ccggttgcgg gagtgggccg atatcttccg
    15721 ggaactccag gaaatcgtga ccgagtccgg catgaaaatc agtaagcgac ggaacgacgc
    15781 agcggcaatc caccgcgcca tcttgacggg gcttctttct aacgtcgcca tgcgtcacaa
    15841 tcggtacgaa tacactgtgg cgagcggggg caaggcgtat ctttggccag ggtcgggcgt
    15901 gtttcagagc aaaccaccct gggttgtcgc tgccgagttg gtcgagacgg ggcggcgcta
    15961 cctgcggata tgtgccaaga ttgacccggc gtgggtcgag gtattggcac cccatcttat
    16021 caaaaagtcc tacgtggacc cgtattggag tcgccgttat ggggcggcca tggtctatga
    16081 aaaagtgacg ctctttggtt tgccggtggt gccgcgacgt cgaacgcctt tgggaccggt
    16141 cgatccgcaa actgcccgcg aaatgctcat ccagcacggc ctggttgaag gggaaatcca
    16201 ttgcgatgcg gccttctttg tccacaatca gcggctcgtt cgcgagatgg aaaccgctca
    16261 ggtaaaactg cggcgagtcg atctcctatt gggacaatgg gcccagttcg atttttacga
    16321 tcagcggatc ccctcccatg tgtatgattt ggacggcctt cttcattggt ggaacgaggc
    16381 gtcaaagagc gatcccaaca tcttgcacat gcggccaagc gatgtggtcc gcgaagaacc
    16441 cccgcccgac atcgacaagc agtttcccga ccatctcttg gtggaaaagg ctccgctgcc
    16501 gctgacatac tgttttcagc caggacaacc caacgacggg gtcaccgtga cggtgccgct
    16561 ggagctcttc cactgcttag atccccgtcg cttggaatgg cttgtgccgg gcatgctgga
    16621 aagcaagatc gctgctctca tcaagtcgtt gccaaaggaa aaacggcggc gactcatccc
    16681 ggtgcaagag accgcccgcc agacccttca gatgctgtct ttcggccaag gtcgattcct
    16741 tccggcagtg gccgaagttt tatcccgctt ggccggggag actattcgtc ccgaggattt
    16801 tcagttggac aaaattcccc cggaattgca catgtcgatc caggttgtcg atgccgaggg
    16861 taaggtggtc gccaagggac gaaactggac ggaactccgc gaaaagttgg ggaccagaca
    16921 ggcggtggca ttttctgcca ttgacgatcc ccattggaat cgggatggac tgaccgattg
    16981 ggactttgat cttttgccgg aggagatcga agttcgtcgt ggcgatattc ccgtcaaggc
    17041 cttccctatg ttggtggacg cgggaaatac ggtttcgctc cgtttggcgg acagccccgc
    17101 ccgggccgcc caccagtcgc ggtttggaat tcgacgcctc cttgccattg ccgtcttacc
    17161 ccatctcgat ccacggtggg atgaattccc ggactgtgat cgcctgcgcc ttatggcggc
    17221 cacgttacca ggtttcgact ttcagaatca actcctgctg gcgctcatcg atcgtgcctt
    17281 tttggacgaa agcctcgtgg gaccgtggca gatcggcgag tggggaaatc tgccccggac
    17341 acgggcagag taccgccgcc tgtttcggat ggggctcaaa cgacttcccc tggccgtgca
    17401 ggaagtcctt tccttgattc gtcccctttt ggaaacctac caccaagcgg cgatcgcgtt
    17461 acaaaatgcc gactccgtgc aatgggaaga atctcgggcg gacatccaag aacaactggc
    17521 ggaattgacc tgtcccggct ttctgacgca cacaccttgg aactggctac gacagtaccc
    17581 ccgatacttc cgcggcatct cccgacggat tgaggccttg cggctgggcg gggtctttcg
    17641 cgatcgtgaa gcgatggccg ttttccgccc gtattgggag gccttcctcc agcgccgtca
    17701 attccatcag gagttggata tcttcgatcc agaactcgtc ctttaccggt ggatgctgga
    17761 agaattccgc atctccctat ttgcccaggc cttggggacg gctatccctg tttcgcccca
    17821 acggctggat cggca
//
LOCUS       DAOSUT010000081        17512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2027_length_17512_cov_9.026809, whole genome shotgun sequence.
ACCESSION   DAOSUT010000081 DAOSUT010000000
VERSION     DAOSUT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 17512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17512
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_17512_cov_9.026809"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="PLD05_08985"
     CDS             complement(<1..>1145)
                     /locus_tag="PLD05_08985"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOP77615.1"
                     /translation="YDVFVAKLDPEGAVLVYSTFLGGTSYDYGYAIAVNAIGNAYVMG
                     YTWSSDFPTTPGAFDTTHNGNSDIFVARVSLGDPIQVEDLGTIDFRALPDLTMGTAEE
                     RLFRMVPMRDGVLTVEALGVGAGETLIGRVYDKNPLTDPQAVLVGEFSTLGGEARLDS
                     EVEGSREYFLYLRGNSANFDLRIANLVSESGGGAIIFGTAGNDVLQVNAATAVVDING
                     VVYDVPDPGDIAFLRFEGEAGWDMLIIHDGPANDVIRLYRDEGTWDSGGVLAPSSWRH
                     EEEVYFYATAGGHDRAEFYDHYLDGTGELPVKFKSEPQYNHAKIVVPGTLYHRVKFLE
                     EVVFFATGENDLGVLFGSPGNDRLEAQWGRTRFQGPGFDVELRDVAF"
     assembly_gap    1146..1245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1246..1705)
                     /locus_tag="PLD05_08990"
     CDS             complement(<1246..1705)
                     /locus_tag="PLD05_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77616.1"
                     /translation="MNVALTDRGPVFAVFRQGATSDNAGPEDLAGENPAEAPATEVLA
                     FSAQFVGARETVPVGRQRAETVFNYFVGPEERWRSEVPSYEVVAYEGLYEGIDLYTWG
                     QRSRLKYEFHVAPGADYRAIQVRYEGITGLSLAEDGSLIVGLGDGWGHVVD"
     gene            complement(1702..1941)
                     /locus_tag="PLD05_08995"
     CDS             complement(1702..1941)
                     /locus_tag="PLD05_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77617.1"
                     /translation="MRNPFTSFTRATMQSIRGNPRLRWGIERQRLQCELLEDRCLLSV
                     GPSQFELFSVMPAVFVENQGQWADASVRMSMTGTG"
     gene            2560..3687
                     /locus_tag="PLD05_09000"
     CDS             2560..3687
                     /locus_tag="PLD05_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77618.1"
                     /translation="MKRNMLWIAAIAWVFSLVALARCGYTQPPWAMGGGPPGMMPGMW
                     GGPQMSQPSQASITGNPEIDRRVQFYEGLMRQMDTNQDGTVSKEEISQSRMGEYLVSR
                     MKERAGVDVSGGFRIDELRQKVIDYYKQRYGGDSQNAQSSSGSQSSTTSSQPQQSLSI
                     LRLGPNVTVPGFGTPTTSSPSSPTVPGFGSPTAGSGTPGTSSGTAPTTSTGSAPQGQV
                     LALPPPQPLDERIRRYAEGLMRQYDENRSGKLEKNEWEKMRGSDWAKADKDGNSELTL
                     QEIGEHLAAQAGGSSSSSSSGKATPVSTKRFIQRSPWDRLPEGLPSWFKEKDRDRDGQ
                     VLMAEFTDKWTQDKLREFQKYDLNGDGVITPAECLKVGKSH"
     gene            complement(3813..4970)
                     /gene="galK"
                     /locus_tag="PLD05_09005"
     CDS             complement(3813..4970)
                     /gene="galK"
                     /locus_tag="PLD05_09005"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOP77619.1"
                     /translation="MTTQSGRVDRQTVAGIFHQTFGKAPDIVARGPGRVNLIGEHTDY
                     NDGFVLPMAIDRAIWIALRPRRDRWVMVHFNDTKQYGEFHLDALRNTGEGSIEYVKGV
                     AWALQEEGFFLRGWEGVVVGDVPVGAGLSSSAALEVVTARVFAAVSHCSWDPVKMALL
                     SQRAENRWVGVQCGIMDQLISACGRADHALLIDCRSLEFQHVPLPPTVRVVILDTGTR
                     RGLVDSAYNERRAACEHTAERFRVKALRDISLDEFERRAVEMDETTRKRVRHVISEND
                     RTLRAAEALRQGDVTTFGRLMVESHVSLRDDFEVSSPALDAMVEIALSQPGCLGARMT
                     GAGFGGCAVALVEAETAETFAQNVAEQYRQKTKHEPAVYISRASDGADIVA"
     gene            5732..7537
                     /locus_tag="PLD05_09010"
     CDS             5732..7537
                     /locus_tag="PLD05_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP77620.1"
                     /translation="MPRASKNRGRTPVSSGKCGKVNGQRVTLPKVTPHNGTGNLPFNG
                     KGDRKNGDDTVPEFLLDTAETAFIKPLAESSLISISVTESRDEDEFDLELAVPEKTPS
                     PSDIDDEDDFEDSEDRELDADATGQDDRIDDPVRIYLMQMGEIPLLSRRDEIRAARDI
                     EKARRKFRYAMLATDYILNAAIELLKKVRDGRLRLDRTMDVSVTNLRGKRHIIHLLGP
                     NLKTLEEIQRRNRRDFRIMMSRRESLENRLAARRRLMKRRFRAVRLVEELDLRTQKLQ
                     PLLDRLTRISQRMDEIVRNLKSLEGTPETPWAKELRRELRYLMRMTLESPTTLRKHLA
                     AINSYAQEYERAKRYLSAGNLRLVVSIAKRYRNRGLSFLDLIQEGNTGLMRAVDKFEY
                     ARGFKFSTYATWWIRQAITRAIADQSRTIRMPVHMIDLCGRIHATARKLAQETGVIPT
                     LDETAQALRMSVEDVRSILRMSHQPLSLDQPVGEQEESYLGEFLADYREDDPLYEMNF
                     ELLKSSIQDALSDLTYREREIIRLRYGLADGYAYTLEEVGKIFGITRERVRQIEAKAI
                     RKLRHPVRSRKLAGFVDALVARGQTTPDPALDREL"
     gene            8339..9241
                     /locus_tag="PLD05_09015"
     CDS             8339..9241
                     /locus_tag="PLD05_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOP77621.1"
                     /translation="MPLVVVGSVALDSVETPHERRENVVGGSAVYFGLAARFFTPVRL
                     VGVVGEDWPPEHTQLLQDHGIDTSGLMTIPGAKTFRWRGRYLPNMNDRETLEVQLNVM
                     SQFDPVLPEAFRRCKFLFLANGSTKMQMRVLDQCVGPDLVVADTMDHWIRNEPEELRE
                     LMQRIDGLVLNDAEAKLLAQDENLVRAGKKILEFGPTFVVIKKGEHGAMFFSRHEMYV
                     LPAYPTSAVVDPTGAGDSFAGGMMGYLAEKNDFSPRTLKEAMAYGVVVASFTVEDFSI
                     DRLLKIRREDIQLRLDEYRKMLMF"
     gene            9534..12272
                     /gene="aceE"
                     /locus_tag="PLD05_09020"
     CDS             9534..12272
                     /gene="aceE"
                     /locus_tag="PLD05_09020"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOP77622.1"
                     /translation="MAQSELNVTGSRTATDGTLPEGAIPSLARDVDPVETQEWLDALE
                     AVIRREGPERAVYLLERLKEKAFRTGVPWVSSATTPYINTIPPEAEPEYPGDRALERR
                     IKSLIRWNALAMVVRANRVSSGIGGHISTFASAATLYEVGFNHFFRGKQGPNPPDFVY
                     IQGHAAPGIYARAYLEGRITEKHLENFRRELAEGGGLSSYPHPWLMPDFWEFPTVSMG
                     LGPIMAIYQARFNQYLADRGILPQARESKVWCFVGDGECDEPETLGAITLAGRERLDN
                     LIFVVNCNLQRLDGPVRGNGKIIQELEGAFRGAGWNVIKVIWGSDWDPLLAKDKDGLL
                     VRRMNEVVDGQYQKYTVMPGSYIRKHFFGVDPRLEALVADMTDEQIRRLKRGGHDPVK
                     VYAAFKAAVEHKGSPTVILAKTIKGYGLGEAGEGRNITHQQKKLNEEELRQFRQRFEI
                     PIPEDQIADAPFYRPPEDSPEIQYLKARRKALSGPLPARRHPMTKLKPPKWDDYAEFF
                     AGSEGREVSTTMAFVRLLARLMRDKNIGRYIVPIVPDESRTFGMEALFRQFGIYSHVG
                     QLYEPVDSDTVLYYREAVDGQILEEGITEAGSMASFIAAGTAHSTHGINMIPFFIFYS
                     MFGFQRIGDLIWAAGDMRCRGFLLGGTAGRTTLAGEGLQHQDGNSHLFALAYPTVQAY
                     DPCFAYEMAVIILDGLRRMYYEEEDIFYYITLMNENYVMPILPMGSTEGICRGMYRLS
                     VHDFGKDRPSVDLMASGAILREALRAQEILNQKFAISSRVFSVTSWKRLLEDAREADR
                     HNRLHPEEPARVPYVQEVLAGTDGSIVAASDYVSAVPLSLAKWIPGDYTVLGTDGFGR
                     SEARPELRRFFEVDAEHIVLAALSSLANRGKYPKDRLPSVIAELGIDPNKPVPWKV"
     gene            12351..>13519
                     /locus_tag="PLD05_09025"
     CDS             12351..>13519
                     /locus_tag="PLD05_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77623.1"
                     /translation="MAEFLAIAAAIFLQGSWPAPEVNEPYYLGKAAHYWNPAWCPRDD
                     FLNSQDAHQVFYFTYGWVAHFLSLPAAAWLGRIITWVLLSEVLRRLARAIGLTGLPLV
                     FAVVLFAAFQEHCAMAGEWVIGGVEAKGFSYVFVFLALAQLVQGRWSATLLLLGVATA
                     FHVLVGGWSLVATVLAWLFEGKRRPPIARLFPAVAVAVILALPGLIPSLALTWGVPAA
                     IIREANRIYTFERLAHHLVPGSFRPQDVAKFLLLTLAFLGLAFLPRNDDGWKRFRHFV
                     LGSLVIAGCGWAIGLTVNIFPDLAAALLRYYWFRLSDVTIPLGTAFVAGFLASHRISL
                     PHPWRDTCLAVVFVFLAYHLGNLVAIRARHGIPRTEWSITMLSAWDEDAAARDEF"
     assembly_gap    13520..13619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13620..13801
                     /locus_tag="PLD05_09030"
     CDS             <13620..13801
                     /locus_tag="PLD05_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77624.1"
                     /translation="RLLTLAAKYQFEYILTVSEPRLNLEIVFAKPDNMFVVYRARRMS
                     QGLSHHGQPSNRNSP"
     gene            complement(13774..15066)
                     /gene="tyrS"
                     /locus_tag="PLD05_09035"
     CDS             complement(13774..15066)
                     /gene="tyrS"
                     /locus_tag="PLD05_09035"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOP77625.1"
                     /translation="MPEINVYEELRWRGLIHQTTDDAVLPGFLMERSRTVYAGFDPTA
                     DSLHVGHLMALMILRRFQKAGHRPIALVGGATGMIGDPSGKSEERRLLSEEELRKNVE
                     AVEKQLKRFLDFEGKETSALLLNNYDWMGKFGYLEFLREVGKHVPVNVMLTKESVKTR
                     LERSDSGLSYTEFSYMLLQAYDFVHLYREYGCEIQVGGSDQWGNITAGIDLARRLIGV
                     QLYGITCPLLTKSDGTKMGKTESGALWLDPQKTSPYQFYQYWINVEDDLVEKCLKFFT
                     DLSREEIASIVAQHREAPENRLGQRRLAQEITRLVHGESGLQAAERATAILFGAEIEQ
                     ISERELDEIFSDVPSRTVSRSQLEGPGLPIVDALCLADLAKTKGEARRTIQQGGAYVN
                     NRRVTDQEVRLTAENLVGDRTIVLRVGKKRYGLLRFEG"
     gene            complement(15187..17382)
                     /gene="glgP"
                     /locus_tag="PLD05_09040"
     CDS             complement(15187..17382)
                     /gene="glgP"
                     /locus_tag="PLD05_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOP77626.1"
                     /translation="MCALEVSSSEEMGSAKEGRLSAQTMYEKCIALSRNLWWTWQPDV
                     INLFRDLDPIRWRQLDHNPVALLAEMTPERLEARVSELALHSRINQAYRRLKEYLSQR
                     PLWGRTHAGVLGSKPVAYFSLEYGVHESVPIYSGGLGVLSGDHCKSASDLGVPLVAVG
                     LFYDQGYFKQHLTVDGWQEEEYIDVDVTKLPLDLCTDAEGKPIIITLETRSSRLLVKV
                     WQLRVGRVNIYLLDTDVEGNSPEDRELTSRLYGGDNRTRIRQELVAGVGGVRALRALG
                     IKPGVYHLNEGHSAFAVLEVMRERMVDDGLSFEEARREVRQCTVFTTHTPVPAGHDRF
                     DPGLMEEHLGPLRDALGISHETLMSLGRVDPTRMDEPFCMTVLALKVSRKANAVSALH
                     GRVTRQMWANLYSWRAEDEVPIGHITNGVHIPSWLAWQMKILYDRYFPERWYERMGEP
                     EVWQSIYNVDPGELWETHLTLKNQLMAFVRRRLSRQCRRRNEPDEVIEAARSILDPNA
                     LTIGFGRRFATYKRATLILRDPDRLARIVNDPERPVQFIFAGKAHPRDIAGKEYIRAI
                     ANLRHDPRFNKRIVFIEDYDINVCRHMIQGVDVWLNTPRRPLEASGTSGQKAILNGAL
                     HMSVLDGWWAEAYDGSNGFAIGNGSSHKSEENTDARDAESLYQVLENQVIPEFYDQDS
                     DGLPRRWVLRMMNSISSLAWRFSSHRMVADYVRLAYLPAACGTSSEMSR"
BASE COUNT         3776 a         5085 c         4759 g         3692 t
ORIGIN      
        1 ataaacgcaa catcgcggag ctccacatca aagcccggcc cctggaatcg tgtgcgaccc
       61 cactgtgcct ccaatcgatc gttccccggc gaaccaaaca gtacccccag gtcattctca
      121 ccggtggcaa agaacaccac ctcctcgagg aacttgacac gatgatacag ggtgcctggc
      181 acgactattt tggcgtggtt gtactgcggc tcggacttga acttcacggg caattcccca
      241 gtcccgtcca ggtaatggtc gtagaactcg gccctgtcgt gtcctcccgc cgtggcataa
      301 aagtaaactt cctcctcatg ccgccaactg gatggcgcaa ggactccgcc gctgtcccac
      361 gtgccctcat cacgatagag ccggatgaca tcgtttgccg gaccgtcatg gatgatgagc
      421 atatcccagc cggcctcacc ctcgaaacgg agaaaggcga tatcgcccgg gtcgggcacg
      481 tcatacacca ctccgttgat atccaccact gcggtcgcag cgttcacctg gaggacatca
      541 ttccctgcgg taccgaagat gattgcgcct cccccgctct ccgacaccag gttcgcaatc
      601 cgcaagtcga aattggcaga attgccccgt agatagagga agtattcccg actaccttcc
      661 acctcgctat cgagtcgtgc ttccccaccc aacgtggaaa attctcccac cagaacggcc
      721 tggggatcag tcagcgggtt cttgtcgtaa acccggccaa tcagggtttc cccggcccca
      781 acacccaaag cctccacggt caagacgcca tcccgcatag ggaccatccg gaaaaggcgc
      841 tcctcggcgg tccccattgt taaatccggt aacgctcgga agtcgatcgt tcccaaatcc
      901 tccacttgaa tcgggtcccc cagacttact ctggccacaa agatgtcgct atttccgtta
      961 tgtgtcgtgt caaaagcccc aggcgtcgtg ggaaagtcac tggaccatgt gtagcccatc
     1021 acgtacgcat tcccgatggc gttcacggca atggcgtagc cgtaatcata ggaggtgccc
     1081 cccagaaagg tcgagtaaac caggacggct ccctccgggt cgagtttcgc cacgaagacg
     1141 tcgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatcc actacatgac
     1261 cccacccgtc ccccaggccc acaatcagcg aaccgtcctc agccaacgac aacccagtaa
     1321 tcccctcgta gcgcacctgg atcgcccgat agtccgcccc cggcgccaca tgaaactcgt
     1381 acttcagccg gctccgctgc ccccaagtgt acagatcaat cccctcgtac aacccctcgt
     1441 aggccaccac ctcgtaacta ggcacctccg aacgccaccg ctcctcggga cccacaaagt
     1501 agttgaaaac cgtctcggca cgctgccgcc ctaccggtac tgtttctcga gcgcccacaa
     1561 actgggccga aaaggcgagc acctccgtcg ccggcgcttc cgcaggattt tccccagcca
     1621 gatcttccgg ccccgcatta tcggaagtgg ccccctgccg aaagaccgca aacaccggcc
     1681 cgcgatcggt cagcgcaaca ttcaccccgt tcccgtcatg gacatccgca ccgaagcatc
     1741 cgcccactgc ccctggttct ccacaaacac cgcgggcatc acactgaaaa gctcaaactg
     1801 cgagggcccc acagagagga ggcagcggtc ttccagaagc tcacactgga gccgttgtct
     1861 ctcaatcccc cagcgcaacc gggggttacc gcggatactt tgcatcgttg cccgggtgaa
     1921 tgaggtgaat ggattgcgca ccgtaccagc tcctcacgaa gagaatgaca agggacctgt
     1981 ttttgccctg gcgctggctc aggaacgcaa tctcgagttt ccatctgatt cccgaagcaa
     2041 cggaggactc ctgcaatcta cctatagaac cccgcctgtc aataacccgt cttagggggt
     2101 tgctctttgt caattgcatc catttggctg tgtgaaaggg gcacaagggg gaatcaagtg
     2161 agcaaaggac gatggtcggt tggggaagac gaagaataag ctttggtgcc tgggagagga
     2221 ggggaaccgg aacatcacta aagcggtctg ggaaacctgg aatgctttaa ttgggtgggc
     2281 caaatggaga aacaacacag gatgccgaag ataaatcttc agccccgggg ttgaatcgag
     2341 gattctcctc cttggaagcc cggggccgcg ggggagcgag caaaccgagg ttttggtgcc
     2401 gggagggcga aacatctggg gcggcggatc gttcattctt ataggggaag gtgcgcgcgg
     2461 aaaccgccag cgtttggtaa aataaatggg gatgggacgg acgccgccga ccgaccaatt
     2521 gcatccgacc atggcaaaca gacatgtagg ggaccgctca tgaaacggaa tatgttgtgg
     2581 attgcggcaa tcgcctgggt atttagccta gtggcactgg cccgatgcgg ctatacgcag
     2641 cctccttggg cgatgggtgg gggaccacca ggaatgatgc cgggcatgtg gggaggccct
     2701 cagatgtccc agccatccca ggcgagcatc acgggtaacc ctgagatcga ccggcgggtc
     2761 caattttacg aagggctgat gcgccagatg gacaccaacc aagatgggac agtgtccaag
     2821 gaggaaatca gccaatctcg gatgggagaa tatcttgtct cccgcatgaa agagcgcgct
     2881 ggggtcgatg tctcaggagg gttccggatc gacgagcttc ggcaaaaagt tatcgattac
     2941 tacaagcagc gatacggtgg cgactcccaa aatgcacaat cctcttcggg aagccagtcc
     3001 tccacgacaa gttcccagcc ccaacagagt ttaagtattt tgcgcttggg tccaaacgtc
     3061 accgtccccg gtttcggtac accgaccacg tcgagtccca gcagtccgac ggttcccggg
     3121 ttcggctctc caacagccgg ttcaggaacg ccggggacgt cctccggcac ggcgccaaca
     3181 acatcgacgg gaagtgcccc tcagggccaa gtcctcgccc tgccaccgcc gcagccgttg
     3241 gatgagcgca tccggcgtta cgccgagggt cttatgagac agtatgacga aaaccgcagt
     3301 ggaaagctgg aaaagaacga atgggaaaag atgcggggga gcgattgggc caaggcggat
     3361 aaggacggga attcggagtt gaccctccag gaaatcggcg agcacctggc cgcccaggcg
     3421 gggggctcgt cttcgtcatc ttcgtcggga aaggccacac ccgtcagtac caagcggttc
     3481 atccagcgct cgccctggga tcgtttgccc gagggtctgc cttcctggtt caaggaaaag
     3541 gaccgggatc gtgacggtca agttctgatg gcggaattca ctgacaaatg gactcaggat
     3601 aagctccgcg aattccaaaa atatgacctc aatggggatg gcgtgatcac cccggctgaa
     3661 tgtctgaaag ttggcaagag ccactaaaac cgggctgtgt ggcaacgaat agggtgttcc
     3721 cgcctgagca cgagggcttg gtacacaacg ggaggcattc ttccaacttg tcgcccgtcc
     3781 aaaatcaacc ggcaacggct cgaccgattg gattatgcca caatgtcggc cccatcgctt
     3841 gcccgcgaaa tgtaaactgc tggttcgtgt ttggtctttt ggcgatattg ttcggcaacg
     3901 ttttgagcaa aagtttcggc ggtctcggcc tcaactaggg ccacggcaca tcctccgaat
     3961 ccggcccccg tcatcctggc acccaggcag ccgggttgac taagggcaat ctcaaccatc
     4021 gcatcaagag cgggacttga gacctcaaaa tcgtccctaa ggctaacgtg gctttccacc
     4081 atgaggcggc caaaagtggt cacgtcacct tgtcgcaagg cctctgccgc ccgaagggtt
     4141 cggtcgtttt ccgaaatgac gtgacggact cgtttccgcg tcgtctcgtc catctcaacc
     4201 gcacgacgtt cgaactcgtc gagtgagatg tcccgcaagg cctttactcg gaaccgctcc
     4261 gctgtgtgct cacacgccgc ccgccgctcg ttgtaggcag agtccaccag gccccgacga
     4321 gttcctgtat ccaaaatgac gacacgtacg gtgggaggga gagggacgtg ctgaaattca
     4381 aggctgcggc aatcgatgag tagggcatgg tcggcccgcc cgcacgccga gatcaattgg
     4441 tccatgatcc cgcactggac gcccacccaa cggttttctg cccgttgcga taggagagcc
     4501 attttcaccg ggtcccaaga acagtgcgaa acggcggcaa agactcgagc ggtgacgacc
     4561 tccaaggccg ccgaggacga caaaccggcc cccaccggga cgtcacctac cacgacgccc
     4621 tcccagccgc ggaggaaaaa gccttcttcc tgcagcgccc aggctactcc tttgacgtac
     4681 tcaattgacc cttccccagt attccgcagg gcatcgaggt gaaactcacc atactgtttt
     4741 gtgtcgttga aatggaccat cacccagcga tcgcgccttg gccgaagggc gatccaaatt
     4801 gcccggtcaa tcgccatcgg cagcacaaaa ccgtcgttgt agtcggtgtg ctctccgatg
     4861 aggttaacgc ggccaggccc cctagcaacg atgtcagggg cctttccgaa cgtttgatga
     4921 aagattcccg cgacggtttg gcgatcaacg cggccagatt gggtagtcat agtatctaat
     4981 tgaaaaccgc gtccccacga tgactgatcg caaccaggat cacatcgtag ccagcatgac
     5041 aggtcggcgg ccgaggacta agctatctat catgggccgc ccaacttttt ccgtcaaccg
     5101 ggacgaggat cgagtgacca ggcgagctga gcgagttggt ccaccaacgg ctataactaa
     5161 aggaaatctt tcccgcgttt gcctaaccgt ttcagcttcc tcacgtggaa tgccttccac
     5221 tcaggcagtg ccggtggtcg ataaaacgac caaacgccgc atggaactgt tgccgcgatt
     5281 ggtcatccca gtcgtttcat tatttacgat tttcctattt ctcccagttg accagatttt
     5341 tctggctcga taagataggg gagttgctgt tttccctaaa ttccccacat tgcgcttact
     5401 gcacgggaga gtgaaaacct ggcggtgggg gcagcgtgag gtggcgctgc cggtattggg
     5461 gtacgctggg aatgcgaact gggatttcca ttggcatttc cggcgagcat caccatgttg
     5521 cctttgatgt ttcagttttg aaacatgatc ggcaccgcga gaccaaatga gtttttccac
     5581 aagaacataa atactttgta gtaaacgatt tacgttttga gcacgtcctg cacggacgct
     5641 caggcattgg atgtgctccc tccttaatca ggcaaaacgg tacgcgtggt tgtggccgct
     5701 ttgaggtctc ttgcctgttg aggacagcac cttgccgaga gctagcaaga atcgcggacg
     5761 cacgccggtc tcatccggca aatgtggcaa agtcaatggc cagcgggtga ctctccccaa
     5821 ggttacccca cacaacggaa ccggcaacct acctttcaac gggaagggag accggaagaa
     5881 tggggacgat acggttcccg agtttctcct cgatacggcc gaaacggcat tcatcaagcc
     5941 gcttgccgaa agcagcctca tctccatttc ggtgacggag tcccgcgacg aggacgaatt
     6001 cgacctggaa ttggccgtcc ccgaaaaaac cccctcccct tcggacatcg atgacgagga
     6061 tgatttcgag gactcggaag accgcgaact ggacgcggat gccaccggtc aggacgatcg
     6121 aattgacgac cccgttcgca tctatctgat gcaaatgggc gagattccgc tcctcagtcg
     6181 acgggacgaa attcgggcgg ctcgcgacat cgaaaaggcc cggcgcaaat tccgctacgc
     6241 catgttggcg acggactaca tcctcaacgc cgcgatcgaa ctgctgaaaa aggtccgaga
     6301 cggtcgtctg cggctcgacc gaaccatgga tgtctcggtg accaatctgc ggggtaaacg
     6361 ccatatcatc catcttctcg gcccaaactt aaagactctc gaggagattc aacggcgaaa
     6421 ccgacgtgat ttccgcatca tgatgagtcg ccgcgagtcg ctggaaaacc gtttagcggc
     6481 tcggcgacgg ctgatgaagc gtcggtttcg ggcagtccga ctggtggaag aactcgatct
     6541 gcggacgcaa aagcttcagc cattgctgga tcggctgaca aggatttcgc aaagaatgga
     6601 tgaaattgtc cgcaatctga aatccttgga aggcacgcca gaaaccccct gggccaagga
     6661 actgcggcgg gaactccgct atctgatgcg gatgaccttg gaaagcccca ccacattgcg
     6721 gaaacatctt gccgctatta actcctatgc ccaggaatac gaaagggcga agcggtatct
     6781 ttcggcagga aacctccggc tcgtcgtctc catcgccaaa cggtatcgca accgaggatt
     6841 gagctttctc gaccttatcc aggaaggtaa cacgggattg atgcgggcgg tggataaatt
     6901 cgagtatgcc cgcggcttca aattctcaac ctatgccaca tggtggattc gacaggcgat
     6961 cacccgggca attgccgacc aaagtcgcac catccggatg cccgtgcata tgatcgatct
     7021 ctgtgggcgc atccacgcca cggctcgcaa gctggcccag gagacgggtg tgattccgac
     7081 cctcgacgag accgcccaag cgctgaggat gtcggtcgaa gatgttcgca gtatccttcg
     7141 tatgagccat cagccattgt cactcgatca accggttggt gaacaggaag agagctattt
     7201 gggcgagttc ctggcagatt accgcgaaga cgaccccctc tacgagatga actttgaact
     7261 gcttaagtcg tccattcagg atgccctttc ggacctgacg tatcgggagc gggaaatcat
     7321 ccggttgcgt tatgggctgg ccgatggtta tgcctacacg ctggaagaag tgggcaagat
     7381 cttcggtatc acgcgggagc gggtccggca gatcgaggcc aaggccatcc gaaaactgcg
     7441 gcatccggtg cgatctcgaa aactggcggg cttcgtcgat gccctcgtcg cccgcggaca
     7501 aaccacgccc gatccggccc tcgaccggga gttgtaagtg gcgagaccga ggggacttgg
     7561 tccctcccac agaatctcgc atgagttgtg gacttggcaa ggcggccgct gaatgaggcc
     7621 tcgccccttc tgcggaatcc tcgcggcctc cgtgttcgta cgatcgctgg gccatgccgt
     7681 aggaaaaaag tcctcgcacg ccgcagcttt cttgatgtcc gcaatttgtg ggagctcaat
     7741 gggttgtggt cgtcgttaag ccctgaaata ttcctgttct atctcgctgg cagaacttgc
     7801 agtgacctgc aatagggttt tcggggtgga aggtgcgagg tctctcgccc ttcctggctt
     7861 gcccaagatc aatgtcgtac ttagagccta tcccataatc tcttcttatc cgttcggcgg
     7921 gagaggtgtc gggggtgaag gtgcctcgaa tcgctgcccg ttttctccat cgatcccgac
     7981 gttcgcttgc cctcacccca accctctccc ggagggagag ggggactttt tgggataggc
     8041 tcttaattga tcgaagttgc gagcaattgc ttggtcatcg cgccggtggc tcacgatagg
     8101 ccgtcctatc attttgacgc tatcggtgac atctccaatt atcggagaaa ttcccgaccg
     8161 ctcctcttcc aattcgcagc acctaatccg ccacgtggcc gacgctagcg aaccacggca
     8221 aaaaccagta ttcttacaaa gacagtgcgg ggcgcgaggc gtcccatacc caaaagggtt
     8281 ttgaacccgg tgattgtcca aatcaagatg agacctgtga gttaggagac ctcgttccat
     8341 gccgcttgtc gtggttggct cggtcgcttt agacagtgtg gagactcccc acgaacgacg
     8401 cgaaaacgtg gtgggaggat cggctgttta ttttggtttg gctgcccgat tttttacgcc
     8461 ggttcggctc gtcggtgtcg taggggaaga ctggcccccg gaacatacgc aactgcttca
     8521 ggatcacggc attgacacgt cgggactgat gacgattccc ggcgccaaga cattccgctg
     8581 gcggggccgt tacctgccga acatgaacga ccgcgagacc ctcgaggtcc agctcaacgt
     8641 catgtcgcaa ttcgatccag tcctccccga ggccttccga cgttgcaagt ttcttttcct
     8701 ggccaacggc tcaacgaaga tgcagatgcg ggtcctggat caatgcgttg ggcccgatct
     8761 ggtcgtggcc gataccatgg accactggat acgtaacgaa ccagaagaac ttcgggaatt
     8821 gatgcaacgg atcgatggcc tggtcctaaa cgatgctgag gccaaattgt tagctcagga
     8881 tgagaatctt gtccgcgcgg gaaagaagat tctcgagttc ggcccgacgt tcgttgttat
     8941 caagaaaggc gaacacggag ccatgttttt cagtcggcac gagatgtacg tcctcccagc
     9001 atatccaacg tcggcagtag tggatccaac gggtgcaggg gacagttttg ctggcggtat
     9061 gatgggctac ctcgccgaaa agaacgactt ttccccacgg acactcaaag aagctatggc
     9121 gtacggcgta gtcgtcgcaa gcttcaccgt ggaggacttc agtatcgacc gccttttgaa
     9181 gattcggcgg gaagatatcc aactgcgatt ggatgaatat cgaaagatgc tcatgtttta
     9241 gaaaagcgct tttgccgaaa acacccacac cgtggcgaat ctcgttccgc ccactgtgga
     9301 agcattcgcc gagtagaaac gatgcatccg ttccggtagg gaaaaaaagt gtttttggcc
     9361 gagttcgtat aattcatggt gtttcaaaca tcgccatgaa gccgaccgat gaatcttcca
     9421 tccggtgctc gggattgggt tgatcggcgg tcgtcaggat cattgccgta gtgtaggctg
     9481 attgggcaag atggactggg tattcttgtg aaatgaaaac gaggcaagtg agtatggccc
     9541 aaagcgagtt gaacgtgacc ggatcgcgta ctgctaccga tgggacctta ccagaagggg
     9601 ctattccttc gttggcccgt gatgttgacc cagtggaaac ccaggaatgg ctagacgcct
     9661 tggaggctgt cattcggcgt gaaggcccgg agcgggctgt gtatctgctg gaacgactga
     9721 aagagaaggc tttccgcacc ggcgtcccgt gggtctcctc ggcaacaaca ccgtacatca
     9781 atacgattcc cccagaggct gaacccgaat atccaggcga tcgggccctg gagcgccgta
     9841 tcaagagcct aattcgctgg aacgccttgg ccatggtcgt ccgcgccaat cgggttagct
     9901 cgggaattgg cgggcatatt tcgacttttg cctcggcagc cacgctttac gaggtggggt
     9961 tcaatcactt cttccggggc aagcaaggtc ctaatcctcc cgactttgtt tacatccagg
    10021 gacacgccgc acctgggatt tatgcgcggg catatctcga aggccgaatc accgaaaagc
    10081 atctcgagaa cttccgtcgc gaattggccg aaggcggtgg cctgtcctct tatcctcatc
    10141 cctggttgat gcctgatttc tgggaatttc ccacggtctc catgggacta gggccgatca
    10201 tggcgattta ccaagctcgg ttcaaccagt atctggccga ccgcggcatc ctgcctcaag
    10261 cccgcgagtc taaggtctgg tgttttgtgg gagacggtga atgcgacgaa ccggagaccc
    10321 tgggggccat caccctagct ggccgggaac gtttggacaa cctgatcttc gtggttaatt
    10381 gcaacctgca acgtttggac ggaccggtcc ggggcaatgg caagattatc caggaactgg
    10441 aaggggcctt tcgcggggcg ggctggaacg tcattaaagt tatttggggc tctgattggg
    10501 acccgctgct ggccaaggat aaagatgggc ttctggtccg ccgcatgaac gaggtcgtcg
    10561 atggccagta tcaaaaatat accgtcatgc cgggaagcta tatccgcaag cactttttcg
    10621 gtgttgatcc ccggctggag gccctcgtag ccgacatgac ggacgaacag attcggcggc
    10681 tcaagcgcgg ggggcacgac ccggtgaagg tttatgctgc cttcaaggcg gctgtggaac
    10741 acaaaggctc gccgaccgtg attctggcaa aaacgatcaa gggatacggg ttaggcgagg
    10801 ccggtgaagg gcgaaatatc acccaccagc aaaagaagct aaacgaagaa gaattgcggc
    10861 aatttcgtca gcggttcgag attcctattc ctgaagacca aattgcggat gcaccttttt
    10921 atcgcccacc ggaagacagc cccgaaatcc agtatttgaa ggcgcgccgg aaggccctca
    10981 gcggtcccct ccctgctcgg cggcacccca tgacgaaact caagccgcca aagtgggacg
    11041 actacgccga gttctttgcc gggagcgaag gacgtgaggt ctctacaacc atggcctttg
    11101 tgcggctcct cgcccgcttg atgcgtgaca agaacattgg gcgatacatc gtgccgatcg
    11161 tgcccgatga aagccgcacc tttggcatgg aggccctgtt tcgacagttc ggcatctatt
    11221 cgcacgtcgg tcaactttat gaacctgtcg attccgatac cgtgctgtac tatcgcgagg
    11281 cggtggatgg tcaaatcttg gaagagggga ttacggaagc cgggtcgatg gcgtcattca
    11341 tcgccgccgg gacggcccat tccacccacg gtatcaatat gattccgttc ttcatttttt
    11401 actcgatgtt tgggttccag aggatcggtg atttgatctg ggcggccggg gatatgcggt
    11461 gccgcggctt cttgctgggc ggcacggcag gacgaacaac cctcgccggg gaagggctgc
    11521 aacatcaaga tggcaacagt cacctttttg ccctcgccta cccaaccgtg caagcatacg
    11581 atccctgctt tgcctacgag atggccgtga tcatcctcga cggtctccgc cggatgtact
    11641 acgaggaaga agacatcttc tactacatca cgctgatgaa cgaaaactac gtcatgccca
    11701 tcctgcctat gggttcaacc gagggtattt gccgggggat gtatcgcttg tcggtacacg
    11761 atttcggtaa agaccggcca agcgtggacc tgatggcaag tggcgcaatt ctgcgggagg
    11821 ccctccgtgc ccaggaaatc cttaaccaaa agtttgccat ctctagtcgc gtgtttagtg
    11881 tcacaagctg gaagcggctt ctcgaagatg cgcgcgaggc cgaccgccat aatcgccttc
    11941 accccgaaga accggcccgg gttccctatg tgcaagaggt cttggcgggg acggatggct
    12001 caattgtggc ggcctcggac tacgtctcgg cagtgccgct ttccctggcc aaatggattc
    12061 ctggcgacta cacggtgttg ggcacggatg gctttggacg tagcgaagcc cgtcccgaac
    12121 ttcgccgctt cttcgaggtg gatgccgaac acatcgtcct ggcagcctta tcgtccctgg
    12181 ccaaccgggg aaaatacccc aaggatcgtt tgccctctgt gattgctgaa ctcgggatcg
    12241 accccaataa acccgttccc tggaaggtct gaccaggtcc ttagccgtgg gcaaaaaccg
    12301 tgcagtgttc cgtgcaaccc ggtatattgt cccgagcgac gctgttaggg atggcggaat
    12361 ttctagccat cgccgcggcc atttttttac aagggtcatg gccagcccca gaggtcaatg
    12421 aaccttatta cctgggaaag gccgcccact actggaaccc cgcttggtgt ccgcgcgatg
    12481 attttttgaa cagccaggac gcccatcagg tcttttattt cacgtacggt tgggtcgccc
    12541 attttctctc gttgcctgcg gcggcctggc tgggacgtat catcacctgg gtactgctca
    12601 gcgaggtcct gcggcgactt gcccgcgcaa ttggactgac tggattgccc ctcgttttcg
    12661 cggtggtcct tttcgcggca tttcaagagc actgcgccat ggcaggcgag tgggtcatcg
    12721 gtggggtaga ggccaagggt ttctcttacg tatttgtttt tctggccctg gcccaacttg
    12781 tgcaaggccg ctggtcggca acactccttc tccttggtgt cgcaacagcg tttcatgtgc
    12841 ttgtgggcgg atggtctttg gtagccacgg tgttggcctg gctattcgaa ggtaagcgac
    12901 gcccaccaat cgccagactt tttccagccg tcgctgtggc agtgatcctg gctcttcccg
    12961 gtctgattcc ctcgctggcg ctgacgtggg gtgtgcccgc cgcgatcata cgagaggcca
    13021 accgcatata cacctttgag cggcttgccc atcacctcgt tcccgggtct ttccgtcccc
    13081 aagatgtggc gaagttccta ttgcttacgc tcgcgtttct gggcttggcg ttccttccac
    13141 gtaacgacga cggttggaag cgcttccgcc attttgtttt aggatcactc gtcattgccg
    13201 ggtgtggctg ggccataggt ctaacggtga acatctttcc cgatctggcg gcagccttgt
    13261 tgcggtatta ttggttccgc ctgtccgatg tgacgatacc tttggggacg gcatttgttg
    13321 cgggcttttt ggcctcgcac cgcatttcgc taccacaccc ttggcgggat acctgtctgg
    13381 ccgtcgtgtt cgtgtttttg gcgtatcacc taggtaatct cgtggctatc cgcgcacggc
    13441 acggcatccc gcgcaccgaa tggtccatca ccatgctatc tgcctgggat gaagatgcgg
    13501 cagcccggga cgagtttgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    13621 ccgcctcttg actctagccg ccaaatatca gtttgaatac atcctcaccg tatcagaacc
    13681 acggctaaat ctggaaattg tgttcgccaa gccagacaac atgttcgttg tctatcgagc
    13741 gcggcggatg tcacagggcc tttcacacca cggtcaacct tcgaaccgca acagtccata
    13801 gcgtttttta ccgacccgca gaacgatggt tcggtcccca accaggtttt cagcagtcag
    13861 acgaacctct tggtccgtga cacgccggtt attcacgtag gcgccgccct gctgaatggt
    13921 gcgtcgagct tcaccttttg tcttggccag gtctgccagg caaagggcat cgactatggg
    13981 caagccaggg ccttccaatt gcgagcgcga aaccgtccga ctggggacat cactgaaaat
    14041 ctcgtctaac tcccgctcgg aaatctgctc gatctcggcc ccaaacaaga tcgccgtggc
    14101 cctttctgcc gcttgcaggc cggattcgcc gtgcaccaat cgggtgatct cctgagccaa
    14161 gcgacgctgt ccgagacgat tctccggggc ttcacggtgt tgagccacaa tggacgcaat
    14221 ctcctcccga gacaagtccg tgaagaattt caggcacttt tcgaccaggt cgtcctccac
    14281 gttgatccaa tactgataga actgataagg acttgtcttc tgcggatcaa gccatagcgc
    14341 cccgctttcc gtcttgccca tctttgtccc atcgctctta gtgaggaggg gacaggtgat
    14401 cccataaagc tgaacaccga tgagccgccg cgcgagatcg atccccgccg taatattccc
    14461 ccattgatcg ctcccaccca cctggatttc gcacccatat tcccgataca gatgcacgaa
    14521 atcataggcc tgcaggagca tgtaactgaa ttccgtgtaa ctcagaccgg agtcgcttcg
    14581 ttctagccgg gttttcaccg attccttggt caacattacg ttgacgggaa catgcttgcc
    14641 cacttcccga agaaactcga gatagccaaa tttgcccatc caatcgtagt tattgaggag
    14701 gagggcggat gtctctttac cctcgaagtc gagaaacctc ttgagctgtt tttcgaccgc
    14761 ctccacattc tttcttagtt cctcctcgga aagaagcctt cgctcttcgc tctttccact
    14821 gggatcgccg atcatgcccg tggcgcctcc cacaagggca atcggccggt gacctgcctt
    14881 ttgaaatctc cttaagatca tcaaggccat caagtggcct acatgcagac tgtctgccgt
    14941 gggatcaaac ccggcgtaaa ctgtgcggga ccgttccatc aaaaagccgg gcaacacggc
    15001 atcatcggtc gtctgatgga tcaacccccg ccagcgaagt tcctcataaa cgttgatctc
    15061 aggcatggct taagtgaaag gatctatgca aaaacttagg aaaggaccgc cgcggcgtgt
    15121 gagcaacgtc tgctacccgc gagaaccttc gatcgacctg cctccgccga caacggccca
    15181 aagccatcaa cgcgacattt ccgagcttgt cccgcaggcc gccggcaagt aggccaaccg
    15241 aacataatcg gccaccatcc ggtgagagct aaatcgccag gccaaggaac tgatggagtt
    15301 catcatgcga agcacccagc ggcgtggcaa gccatcactg tcttggtcgt aaaactccgg
    15361 aatgacctgg ttttccaaga cctgatacag gctctcggca tctctggcgt cggtgttctc
    15421 ctcgctttta tgactcgacc cgttaccaat ggcgaagccg ttggaaccgt cgtaggcttc
    15481 ggcccaccaa ccatcaagaa ccgacatgtg aagggccccg ttaaggatgg ccttctgccc
    15541 actggttcca ctggcctcca aggggcgccg cggcgtgttt agccagacgt ccaccccctg
    15601 aatcatatgg cgacagacgt taatgtcgta atcctcaata aacacgattc gcttgttgaa
    15661 ccggggatcg tgtcgcaagt ttgcaatggc ccgaatgtat tctttcccgg cgatgtcccg
    15721 cggatgggcc tttccagcga aaatgaactg cacaggtcgt tcgggatcat tgacgatccg
    15781 cgccagtcga tcgggatccc gcaagatgag cgtcgcccgc ttgtatgtgg cgaaccgccg
    15841 cccgaagcca atcgtcagtg cattgggatc aagaatggac cgcgcggcct cgataacctc
    15901 gtcgggttca ttccgccgcc ggcattgtcg gctgagccgt cgccggacaa aggccatcag
    15961 ttgattcttc agcgtcaaat gcgtttccca caattcgccc ggatcgacat tataaattga
    16021 ctgccagacc tcgggttcac ccatgcgttc ataccaacgc tcggggaagt atcgatcgta
    16081 gaggattttc atttgccagg ccaaccaact ggggatgtgc acaccgttgg tgatatggcc
    16141 aatgggaact tcgtcttccg cccgccagga ataaaggttg gcccacattt gccgtgtcac
    16201 ccgaccgtga agggcactga cggcattcgc cttgcgtgac accttcaggg ccagcaccgt
    16261 catgcaaaac ggctcatcca tgcgggtggg atcgactcgt cccaggctca tcaacgtctc
    16321 atgggagatt cccaaggcat ctcgcaacgg accgaggtgt tcctccatca gtcccggatc
    16381 aaaccggtcg tgacccgccg gaacaggcgt gtgcgtggta aacacggtgc actggcggac
    16441 ctcccgacgg gcttcttcaa agctcaaccc gtcgtccacc atccgttcac gcatgacctc
    16501 gagcacggca aaggcgctgt ggccttcatt caggtgataa actcccggct taatccccag
    16561 cgcccgtaac gcacgaaccc ccccgacacc tgccaccaat tcctggcgaa tccgggtccg
    16621 gttgtcacca ccataaagcc gactggtcaa ctcacggtct tccggactat ttccctcgac
    16681 atccgtatcc aagagataga tattgacccg ccccacccgg agctgccaaa ccttaactag
    16741 taaccgagag ctccgcgttt ctagggtgat gatgatcggc ttaccctcgg catccgtgca
    16801 cagatcgagt ggcaacttgg tcacatcgac atcgatgtac tcctcctcct gccagccgtc
    16861 caccgtcaaa tgttgcttga aatacccctg gtcatagaac agccccaccg cgaccaaggg
    16921 aacccccaga tcactagcgc tcttacaatg gtcccctgat aggacgccca gacctcccga
    16981 gtagatgggc acagattcgt ggaccccgta ttccagagag aaataggcca ccggcttgga
    17041 accgaggacg ccggcatgag ttcgtcccca cagtggccgt tgcgaaaggt actccttcaa
    17101 tcggcggtac gcctggttaa tgcggctgtg cagggccagt tccgaaaccc gcgcctctag
    17161 ccgttccggc gtcatttccg ccaaaagtgc caccggatta tgatccaact gtcgccaacg
    17221 gatagggtct agatcacgaa agaggttgat gacatcgggc tgccaggtcc accacagatt
    17281 gcgcgacaag gcaatgcact tctcatacat cgtttgggcc gataagcgac cttctttcgc
    17341 ggaacccatt tcctcgctgc tggaaacctc tagtgcgcac ataaaggttc gctccacaaa
    17401 ggttactgat caagggatgt caacgggaat cccgtccacc ggttggccac acttgcagca
    17461 actgccacac taccttttta ccagactgtg cccaactgtt gacgggcttc tc
//
LOCUS       DAOSUT010000082        17397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2041_length_17397_cov_9.847884, whole genome shotgun sequence.
ACCESSION   DAOSUT010000082 DAOSUT010000000
VERSION     DAOSUT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_17397_cov_9.847884"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..1286
                     /locus_tag="PLD05_09045"
     CDS             516..1286
                     /locus_tag="PLD05_09045"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP77627.1"
                     /translation="MMCRWYSVLALVLSLMPFRAIDTFGEEQGTVGQSQEKAPVRCLI
                     VTGEDYPGHKWRETTPVLKSALERQGQISADVAEDLNVLRTDQPYQYDVIVMHFKNYD
                     PNVPGREGFQKLAAFVRQGGGLVLVHFACGAFQEFKDDFVQIAGRVWDPQLRGHDPHG
                     TFTVKIVDPQHPITHGMKDFETVDELYTCLAGEVAIHILATAKSKVDGKDYPMAFVLE
                     VERGRTFHCVLGHDVKAFASPEVQELLRKGCLWATRRL"
     gene            1401..2279
                     /locus_tag="PLD05_09050"
     CDS             1401..2279
                     /locus_tag="PLD05_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP77628.1"
                     /translation="MQISFHTDAFNSAYWNFERCLKWAEANDVHFIECGVIDGVSWIH
                     GLGYQPHVALYEDPLLLRRKMENYGVRFSQIDAAYPLSGRDGPLYGVPYVLKAIPWAK
                     HAGCPRIATTDGLHQPPGISDEEAMALMKRSYEQIIEVAEAYEITINIEVHGYFTTNP
                     DRLAEMLEFCQSPFLRMNLDTGNSFIAGRDPVEFCRRFREKINHVHVKDVSSSLAAAV
                     RGQETGIAVSHCAIGDGVNAENIRQVLMILRDYGYNGPLSMECEGQGGPMIERSLRWL
                     RATLQELGIPEEKSVP"
     gene            2371..3711
                     /locus_tag="PLD05_09055"
     CDS             2371..3711
                     /locus_tag="PLD05_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77629.1"
                     /translation="MSRGKQASGGDLSRRDFLKTGAVAAGGMAFGTALHRVVHASGSD
                     IIKVGMIGCGGRCSGAAAQALNTGPDVRLVAMTDVFESKVQAALQNLKQQFPDKVVVD
                     QDHCFTGLDGYKKVIEASDVVLIACASKYHPMYSEEAIKAGKHVFVEKPHGIDPVGVR
                     RMQAACDLAKEKGLSIVSGLQSRFDIGWQECMKRIHDGIIGDIVAVQAMFLRGPYQVM
                     TRKPGQSEVEFQYWNWYHFCWLSGDDVPQSLVHNMDRVTWALKEEMPEWCFGLGGRSA
                     SFGEGLGDMYDHHTVVYEYKSGARVYAMCRTQNGCYGNASDIIMGTKGTCYLGQKRIV
                     GETNWRYEGPQNNPYQDEQQALFDSIRKGQPINSGYHMAKSTMVTVLGQLAVYTGQAM
                     RFEDVAKMDFQYRPTVEEARLDMEPPTVPDETGNYPLPKPGISSLEWIAGPRRT"
     gene            3923..5461
                     /locus_tag="PLD05_09060"
     CDS             3923..5461
                     /locus_tag="PLD05_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOP77630.1"
                     /translation="MLAKLKTFTLLGIDALPVVVEVDVSPSGLPKTVIVGLPEASVRE
                     STHRVERAMVNSGFHRANARTVINLAPADLPKEAASFDLPIGLGILAASGQLTPERLA
                     EYAVVGELALDGTLRPVRGVLSMAMQLKKEGGCRGMIVPVANAPEAAIVEEIDVIPVS
                     SLSQAAAFLCGEIDIPPQPSRLEEFFDKAAHYEEDLADVKGQEMAKRALVVAAAGGHN
                     LLMIGPPGSGKTMLARRLLTILPELTPAESIETTRIYSAVGKVNPEQPLLATRPFRAP
                     HHTISDAGLVGGGSIPGPGEISLAHNGVLFLDELPEFNRRTLEVLRQPLEERSVTIAR
                     ANFSSTFPANFMLVAALNPCPCGYRNDPRRQCHCTIPQIERYMSKISGPLLDRIDIHL
                     EVPAVPFHDLMASRPGLGSAEARAMVTQARRIQMQRLNGTKARCNAEMTHQQLRRFCR
                     LSPGSRALLQAAVNELGLSARAHDKILRVARTIADLEGAEEINEMHLSEAIQYRTLDR
                     SFFK"
     gene            5640..6998
                     /locus_tag="PLD05_09065"
     CDS             5640..6998
                     /locus_tag="PLD05_09065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP77631.1"
                     /translation="MADHEADEPHESEPSSVWSSREYRIYAPSWLVTVLAAPIETVVI
                     GIYVYGKTADPLSLGWIGLAKAVPMILLALAGGHLADRFNRQWLLVVTVIGQAFCGFA
                     LAAIAYYQLALWWVYAILVIEAATQALGTPARAALIPLLVSQRQFSRAVAWNSSIFQL
                     GTMIGPAVGGILLGRTKSYALVLGLVGVARAIAVVGFLLMRPRKQEFVSAQESAGKDI
                     WDGLKFVWKHKSILGAISLDLFAVLLGGVTYILPIFAEDILKVGPEGLGFLRSAEALG
                     AITMAMILAHLPPMRHAGRNMLLAVTGFGLATIVFGLSKSFWLSLAMMFLIGALDNIS
                     VVVRHTLVQMLTPDRMRGRVSAVNSVFIVISNDLGGLESGVTARFLGAIGSAILGGTG
                     AIVAVLIAMKLWPELTKIGRLDDIRTVEEGLSPGLERVEPVACRELSNNVQTVSTGSA
                     SH"
     gene            7468..13455
                     /locus_tag="PLD05_09070"
     CDS             7468..13455
                     /locus_tag="PLD05_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOP77632.1"
                     /translation="MSNHKNKSQWSLAFILEVVIGCCCLLGSMSVARGQVIVRQWEFN
                     RSGDAEGWKAGGHLSDTHVADGVLRTKVIDWDPILVHDVFEPPLPATATQVIEIRLFA
                     PHAGQAEFFWTNTTEGQYGGFAPGKETPFAVKPGWNTYRVQPFWQAEGKIVRLRFDLP
                     SRTANEEPSEYQIDYIRILELAGEGPPGPAEWDFAQGLAGWQLEGDGKLWWENGCLNA
                     EVQPGARLVAPPIQADADQLAFVAMEMAVDQPGSARLIWASGKVNGQQSTGFTLRAGE
                     RFAVYNLPVAGTSNWKTPVIYLALELTSNHRPVRLRLKWLKLSEEPGGPPDLELERFF
                     VKDALPRVDKVCNVVAHVTNRGGEPVRDVRAELILPDGAEFVDSASPLRHLAELEFGE
                     TEALVWKIKNRRVGPVSLRLRIVSPELEEYERQEEFLPDLRLPREDYVPEPKPVRGPF
                     EVGVYYFPGWGRPSSWQPIERFPERRPVLGFYREGLPEIADWHIKWAVEHGITFFCYD
                     WYWRQGERRLEHALHDGFFQARYRHLMKFCLLWANHFGPGEHSAEDNERVCKYWIDNY
                     FRRPEYFKVEGRPLLVIFSVHSMQRDLGIEGTRKAIELWHRMTEEAGVGKILVAGCGT
                     PANLKQMKEMGFDAVTGYNWPSCGIQDRNWVPFSEVAANYDALWWRPLAEAGAMPVIT
                     PVSAGWDSRPWHGDRALVLTDCTPQAFQQHLEDAKRFIEETGQPRVLLIEAWNEFGEG
                     SYCEPHKQYGFGHLEAIRKVFAPDSQPPLHYGPEDVGRPLLEFRDLEKPVSRTQWNFD
                     TPDQDEGWKPLMGLTDVTVANGVLAARTISGDPAFHCSTKLRAKDFAGIEITIAMRSS
                     KPTDIVQLFWATLSNTYREEASVRTTVVCDGKMRTYRLDLAGHPLWRGVITGFRLDVG
                     SQPDAEIKIDSIRLIRSASEAFENLRRAIDDLIATFGASYPEGQEFRRRLDELEQLHR
                     KGQPVEKALSELQRKALLANPLLDFDRLLVLKRKRGQLGLPTNHQCNSCLDQTGYDNE
                     LAIFSWRDGTLKTLYRPPQGFYVGEVDLHWDADRLLFTMPTGQTWQIYEMRLDGTGLR
                     RVSREEPGVDNFDACYLPDGGIAYASTAAFTAVPCWHGRERACAIFRMEADGSGVRQL
                     CFDQDLDLHPAVLPNGQIIFSRWDYTGIMHIYLRPLMVMNPDGTQQRAIYGSNSYYPN
                     ALYFPRAVPGQASKLVAILSGYHGVNRAGDLVLLDYGRGWHEAKGIVHRFMHGREPIE
                     PIIRDDLVGPNWPKFLHPYPLSENYVLVSAQLHQKGGWGIYLVDRFDNMIPILVDEQY
                     DFFEPIPIRKQPRPPVIPPRVDLSDPTAVVFLQDVYRGPGLKGVPRGTIKNLRIVAYH
                     WGFPGMAGPDKVGRAGPWDVMRILGTVPVEEDGSALFRIPANTPIAIQALDENGAAVQ
                     IMRSWYTAMPGEQTSCIGCHEPPTEAPQQLTRLVGMREPVDIKPWYGPPRGFDFEREI
                     QPVLDKYCVGCHDGQGNDGGRLDLRALRFHPDYQGLPLSQLGASRLWPEVAALLPSCE
                     PPHRLLGNKRYPYTPAYEALIPFVRRVNIEDDVYLLNPCEYSADTSELIQMLKKGHYG
                     VELDPEAWDRLQTWIDLNGPCHGRWGVVCPVPQEANKKRLAILKQSGGPDIDPEELPD
                     IRFDSQVAKIPERTTRERPPRPQLDTFPLSPDAALARQQERPWTMTITVSPGIDLELV
                     RIPSGRFVMGDPSGDDDENPACVVSVEKDFWISRYEITNEQYRCFDPAHDSRVFTKRS
                     INVNGPGVAMNEAKQPVVRVSWEEAMSFCRWLSERTHLRFRLPTEAEWEYACRAGTAT
                     AFFFGDRDSDFSQYANLADAAIERIYTTTGGVVVLQPIPADTRWDDGAIATKQVGSYR
                     PNAWGLYDMHGNAAEWTLSLYRPYPYREDDGRNDLTVSGFRVVRGGSFYDRPQRARSA
                     FRLFYRQWQRVHNVGFRVVCQIQDQSDLPVALRSLSQ"
     gene            complement(13544..13993)
                     /locus_tag="PLD05_09075"
     CDS             complement(13544..13993)
                     /locus_tag="PLD05_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOP77633.1"
                     /translation="MSAQDDCRKKIAANDSRAQPSSEGSATHLTPEEVHARLRGLPGW
                     ELESDGKRIFRQWTLKDFATALRFFMEVGQLAEVENHHPDLHLQSYRHVRIVLWTHAV
                     GGLTPLDFQLAEKDQRPGHRVQLGLTCRIKSVSHNLPSLVRRERGRG"
     gene            14309..15655
                     /locus_tag="PLD05_09080"
     CDS             14309..15655
                     /locus_tag="PLD05_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOP77634.1"
                     /translation="MQESRSLTTTEMILDSVADGVFTVDHEFLITSFNRAAEQLTGVP
                     RSDALGRPCCEVLRASICESACALRQTLATGRPIVNKAVFILDSHGNRVPISISTAVL
                     LDANGNVIGGVETFRDLSFAEQLYRELESSDRFVDIVGRSRAMRQIFQLLPQIAESPS
                     TVLIEGPSGTGKELIARAIHRLSPRRNKPFVAVNCSALPDTLLESELFGYKAGAFTDA
                     KHDKPGRFALADGGTLFLDEIGDISPALQSRLLRVLQERVIEPLGGIEPVPVDVRIIA
                     ATHRPLVKLVRKGLFREDLFYRINVVRIELPPLRERREDIPLLIEYFVSKFNRLHGKG
                     ILGVSDEVLARLMDYDYPGNVRELENIIEHAFVLCRGHLIELEHLPPSVRGEGDLRDR
                     SLTKGLTLKEMERVLIRDALRRHQGHRVAAARELGIHPSTLFRKMKSLGITWPEPG"
     gene            15828..16235
                     /locus_tag="PLD05_09085"
     CDS             15828..16235
                     /locus_tag="PLD05_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOP77635.1"
                     /translation="MRIAISDHQGRVSPVFDVATHVTVVEYEQEKEMSQVGYDLEIAD
                     PYRRAEFLANLQIDALICGAVSRPLELLLIEKGIAVYGRVCGDVAEVLRAFMLGKLTG
                     PNFRLPGCPEEGPRRWRRGCRRGRHMSNNEETS"
     gene            16232..16576
                     /locus_tag="PLD05_09090"
     CDS             16232..16576
                     /locus_tag="PLD05_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOP77636.1"
                     /translation="MKVVVTAQGTTLDSPVDPRFGRAATFLLVDTETLEAVAHENTAA
                     GEPQGAGTQAARAVIMLGPQAVITGHVGPRAFAVFQAAGIPIFLAERGTVREAIAAFK
                     AGQLALADHPNV"
     gene            16693..17076
                     /locus_tag="PLD05_09095"
     CDS             16693..17076
                     /locus_tag="PLD05_09095"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOP77637.1"
                     /translation="MPGGDRTGPMGAGPRTGRGMGYCAGYGVPGFMNRPGLWGWFTGW
                     LPGCGRGGRGFRHWFYRTGLTGWQRFAMGWPAWGGVVPPTVPPSPQSELDILRQQAES
                     LSQMLEQIQRRMEELEKRGQSGETA"
BASE COUNT         3672 a         4501 c         5191 g         4033 t
ORIGIN      
        1 cgcgtttcca ttcaggtgcc gcttgcccag gtggctcggt cagcgagcaa gagcgcgagg
       61 ccctcatcca aatgattact gatcgcgtta tggaaatttt ggcctcccaa aggtaattca
      121 taccggttgt ccgaactata tcttacttag agcctatctg aaaaagtcgc cctctccctc
      181 tgggagaggg ttggggtgag ggcaagcgga cgtcggaatc gattgagaaa accggcagcg
      241 attcgagcca ccctcacccc cgacccctct cccgccgaac gggcgagggg agactatgct
      301 tagggtaacc cgcaggaaag cctgcgggtt atttttttgc ggtgactcga tttgctgaaa
      361 aactggaacg catgcttgac ggggcaaaag cgtcggctat gctatcggtc gaagtgtttc
      421 gagagagata atacttcgag cgcgttatgc aagagatcgg gagtccctcg cattagggcg
      481 ccgtcgtgtg acttgctcaa gaagggatcg tggccatgat gtgtcgttgg tacagtgtgt
      541 tggccctggt gttaagcctg atgcctttta gggcgataga cacgttcggc gaggaacagg
      601 gaactgtggg acaatctcag gagaaggccc ctgttcgttg tctcattgtt acgggcgagg
      661 attatcccgg acacaaatgg cgggagacaa cgccggtctt aaagtctgct ttggaacgtc
      721 aggggcagat cagcgctgac gttgccgaag acctgaatgt cttgcggacg gaccaaccgt
      781 atcaatacga cgtaatcgtt atgcacttta agaactacga tccgaatgtg ccaggtcgcg
      841 aagggttcca gaagctggcc gcgtttgttc ggcagggggg tgggctggtc cttgtgcatt
      901 ttgcctgcgg cgctttccag gaattcaaag atgactttgt tcaaattgct ggtcgtgtgt
      961 gggatccgca acttcgtggt cacgatcccc acggaacgtt tacggtgaag atcgttgacc
     1021 cccaacatcc catcacgcac ggcatgaaag acttcgagac ggtggacgaa ctctatacat
     1081 gtttggccgg tgaggtggca atccacatcc ttgccacagc gaagtcgaaa gtggatggaa
     1141 aggactaccc catggccttc gtgctggagg tagaaagagg acgcaccttc cactgtgtgc
     1201 tggggcatga tgtgaaagcc tttgcgtcgc cggaagtcca ggagcttttg cggaagggtt
     1261 gcctttgggc aacacgccgc ctgtgaccta gatggggcca cacccaggga atagcctaag
     1321 agctggtgtt ggccgtctcc aaaatgacgg gaggccgcga aaagctttgg tgcttgacaa
     1381 gagacaaggg agtcgatgcg atgcagatca gtttccatac cgatgccttt aactccgctt
     1441 attggaattt tgaacggtgt ttgaaatggg cggaggctaa tgatgtccat ttcattgaat
     1501 gtggcgtgat tgatggcgtg agttggattc atgggttggg ctatcagccg cacgttgccc
     1561 tgtatgaaga cccattgctc ctgcggcgaa aaatggagaa ttacggcgtt cggttctcac
     1621 aaattgatgc tgcctatcct ctttccggtc gggatggacc gctttatggt gttccctatg
     1681 tcttaaaagc gattccctgg gcaaagcatg ctgggtgccc ccgaattgcc accactgatg
     1741 ggttgcatca acctcccggc atcagtgacg aagaggccat ggcccttatg aaaagaagtt
     1801 acgagcagat cattgaggtt gctgaggcct atgaaatcac gatcaacatt gaggtccacg
     1861 gctattttac gaccaatcct gatcgcttgg ccgagatgtt ggagttctgt caaagtcctt
     1921 tcctgcggat gaatcttgac acggggaatt cgttcatcgc cggacgtgat cccgtggaat
     1981 tctgccgacg ctttcgtgag aagatcaacc acgttcacgt gaaagatgtc agttcttcat
     2041 tggccgcagc cgtccgcgga caggaaaccg gcattgccgt aagtcattgt gcgatcggcg
     2101 atggcgtcaa cgccgagaac attcgccagg tcttgatgat cttgcgggat tacggttaca
     2161 acggcccgct gagtatggaa tgcgaagggc aaggcggtcc tatgatcgag cgatcgcttc
     2221 gctggttgcg ggcgactttg caagaattgg gaattcccga agaaaagtcc gtcccgtaag
     2281 gaaggtggtc atccggggca aattggtgat gttgtatatc cgtgaagatc gtccgtttgg
     2341 ttggctcagt tgatttggag ggaccatgtt atgagtcgtg gcaagcaggc gtctggtggt
     2401 gatttgtcgc gtcgcgattt cttgaagaca ggggccgtgg ccgcgggggg gatggccttt
     2461 ggaacggcac tccatcgcgt ggtccatgcg agtggctcgg acattattaa ggtggggatg
     2521 attggctgcg gtggtcgctg tagcggggcg gccgctcagg cactcaatac agggcccgat
     2581 gtccggctgg tcgccatgac ggatgtgttc gaaagcaaag tccaggcggc gcttcagaat
     2641 ctcaagcagc aattccctga taaagtggtc gtcgatcaag atcattgttt cactggtctg
     2701 gacggttaca aaaaggtcat cgaggcctct gacgtggtct tgattgcttg tgcctccaag
     2761 tatcatccaa tgtactcgga agaggccatc aaggcgggca aacacgtctt cgtcgagaag
     2821 ccccatggta ttgatccggt gggtgttcgc cgaatgcagg cggcttgtga tctagcgaag
     2881 gaaaagggtc ttagcattgt gtcgggcctg caaagccggt ttgacattgg ctggcaggaa
     2941 tgcatgaagc gcatccatga tggcatcatt ggcgatatcg tcgcggtgca ggccatgttc
     3001 cttcgtgggc cgtatcaggt catgacccgc aaacccggac aatcagaagt cgagttccaa
     3061 tattggaact ggtatcactt ctgttggctt tccggtgatg atgtgccaca gtccctggta
     3121 cataatatgg atcgtgtcac ctgggccttg aaagaagaaa tgccggaatg gtgctttggc
     3181 ctcggtggtc ggtcggcctc ctttggcgaa gggcttggcg atatgtacga ccaccacacg
     3241 gttgtttacg agtacaagag cggtgcgcgg gtgtatgcga tgtgccgcac gcagaacggc
     3301 tgctatggca acgccagcga tatcatcatg gggaccaaag ggacctgtta tctcggccaa
     3361 aaacggatcg tcggcgagac gaattggcgt tacgaaggtc cacaaaataa tccttaccag
     3421 gatgagcagc aggccctttt cgactcgatc cggaagggcc agcccatcaa cagcggatat
     3481 cacatggcca aaagcaccat ggtgacagtc ctgggacaac tcgccgtcta tactggtcag
     3541 gcgatgcggt ttgaagacgt ggccaagatg gacttccaat atcggccgac ggtcgaggaa
     3601 gcccgcctcg atatggaacc gccgacggtt cccgatgaga cggggaatta tccgttgcca
     3661 aagccaggga tcagcagttt ggagtggatt gccgggccac gccggacttg atcattgaag
     3721 gcggagcacg gaccgcgtta atcccagtgg ccgtgccccg ctaataacag acaggacgat
     3781 gcccaaagga aaaaggcctc caaatccaag gtttgggggc ctttccttgt tagcatccga
     3841 caccttgcct aggtttcccg gccttctgaa aataggagac attgtcattt aatggaaagc
     3901 caaaccaggg ggcaaccagg ttatgctcgc caagcttaag acatttaccc tattgggaat
     3961 cgatgccctg ccggtggtcg tggaggtcga tgtctcgcca tccggattgc ccaagactgt
     4021 gattgtgggc ttgcccgagg cctcggtgcg agagagcaca catcgtgtgg agcgggcgat
     4081 ggtcaattcg ggctttcacc gggcgaatgc ccggaccgtg atcaacctgg cacccgcgga
     4141 tcttcccaaa gaggcagcct cgtttgacct tcccattggc ttggggattc tcgcggccag
     4201 cggtcaactt accccagaac gtttggcaga gtacgcggtc gttggagagt tggcgctcga
     4261 cgggacgctc cgtcccgtgc gcggtgtcct ttccatggcg atgcaattga aaaaagaggg
     4321 tggctgccga ggcatgattg tgcccgtcgc caatgccccc gaggctgcca ttgtggagga
     4381 gatcgatgtc atccccgttt cctcactcag tcaagccgcc gcatttctct gtggtgagat
     4441 tgacattccg ccccaaccct cgcgtcttga agagtttttt gataaagccg cgcactacga
     4501 agaagacctg gcagacgtta aaggccagga gatggccaaa agggccttgg tcgtggcggc
     4561 tgcgggaggg cataacctcc tcatgatcgg gccgcccggc tccggaaaaa cgatgctggc
     4621 caggcggctg ttgacgatcc ttcctgaact aacgcccgcc gaatcgattg aaaccacacg
     4681 tatctacagc gcggtaggga aggtcaatcc tgagcagccc ctattggcga cccggccctt
     4741 tcgggccccg caccacacca tcagcgacgc gggactggtg ggcggaggat cgatccccgg
     4801 tcccggcgaa attagcctcg ctcataatgg cgtccttttt ttggacgaac tgcccgaatt
     4861 caatcgtcgg acactggagg tgcttaggca accacttgag gaacgcagcg tgaccatcgc
     4921 acgggccaat ttcagctcca cttttcccgc caattttatg ttggtggcgg ccctcaatcc
     4981 ctgtccctgc ggctatcgca atgatcctcg gcggcaatgc cattgcacca ttccacagat
     5041 tgagcgttac atgtcaaaaa tcagtgggcc tttgctcgac cggatcgaca tccatctgga
     5101 ggtcccggcg gtgccgtttc acgacttgat ggcctcgcgc cccggacttg gcagcgcgga
     5161 ggcccgcgcc atggtgactc aggcacgaag gattcaaatg cagcgactta acgggaccaa
     5221 agcacgttgt aacgccgaga tgactcatca gcagctccgt cggttttgtc gccttagccc
     5281 cggttcgagg gcgcttctac aggcggcggt gaacgaattg gggctttcgg cacgagccca
     5341 tgataagatc ttgcgtgtgg cgaggaccat tgccgacttg gagggggctg aggaaatcaa
     5401 cgaaatgcac ctcagcgagg cgatccagta tcgaaccctc gaccgcagct ttttcaagta
     5461 atgtgagaag tttgggaccg gtcgtcggaa agccggaaat cccttataat accgaaaatt
     5521 gcagatcgtg ttttcctcac cgagtcgcga gttgctgttt catgcgatgc tgcaatgtat
     5581 tcttccgcct cgaagtgggc gaaatccttt ttaagcaatt gtaagcatct tgagcgtcta
     5641 tggctgatca cgaagccgac gaaccccacg agtcagagcc ttctagcgtt tggtcttcac
     5701 gcgaataccg gatttatgcc cccagttggt tggtgaccgt cctcgcggct ccgatagaga
     5761 cggtcgttat cgggatttat gtttacggaa agactgccga tcccctttcc ctggggtgga
     5821 tcgggttggc caaggctgtg cccatgatcc tcttggcctt ggccggggga catctggccg
     5881 accgttttaa tcgacagtgg ctactcgtag tgaccgtcat cggacaggcc ttctgcgggt
     5941 tcgccttggc cgccattgcg tactatcagc ttgccctttg gtgggtctat gcgattctcg
     6001 ttatagaggc cgccacacaa gctctgggaa cccctgcccg cgcggccttg attcccctgt
     6061 tagtatcgca gcggcagttc agccgggcgg tggcctggaa tagtagtatc tttcaactgg
     6121 ggacaatgat tggccccgcc gtgggaggga ttcttctcgg tcgaaccaaa agttatgcgc
     6181 tcgtgttggg tttggtgggt gttgcgcggg caattgcagt ggtgggattc ctcctcatgc
     6241 ggcctcgcaa acaagaattc gtctccgccc aagagtccgc gggcaaagac atttgggacg
     6301 ggcttaaatt cgtctggaaa cacaagtcta tcctgggcgc aattagcttg gacctgtttg
     6361 ccgtgctcct cggaggcgtg acctatattc tgcctatctt tgccgaagac attctgaaag
     6421 ttggccctga aggtctcggc tttttgcggt ctgcggaggc cctgggggcc atcaccatgg
     6481 ccatgatcct cgcccacctt ccaccaatgc gtcatgcggg tcgtaatatg ctcctggccg
     6541 tcacgggatt tggcctagct acgatcgtgt ttgggctgtc caaaagcttt tggctttccc
     6601 tggcgatgat gttcctcatc ggggctttgg ataacattag tgtcgtagtc cggcacacgc
     6661 ttgttcagat gttgacaccg gaccgaatgc ggggacgcgt ttcggcggtt aatagtgtgt
     6721 tcatcgtgat ctccaacgac ctcggcggcc tggaatccgg ggtcacggcg cgttttttgg
     6781 gtgctatcgg ttcggccatt cttggtggca cgggggcaat tgtggccgtc ctcatcgcta
     6841 tgaagctttg gccggaactc accaagattg gccgactgga tgacatccgc acggtggaag
     6901 aagggctgtc acctggcctt gaacgggtgg aacccgtcgc ctgtagagaa ctttccaaca
     6961 acgtccaaac tgtgtcaacg ggttcggcgt cgcattgata attgcgaccg attttgaagc
     7021 ccttagcgcc taagccataa tctcccctcg cccatttggc aggagagggg tcagggggtg
     7081 agggtggctc gaattgctac gggttttctc aatcgattcc gaagatcgct tgtcctcacc
     7141 ccaaccctct cccggaggga gaggggggga ctttctggga tagactcttg gttacggcct
     7201 caaggatagt tggccggact agcggcagtg cgaacgacgc ccgagggcct tgatcgcgct
     7261 cttcgtcaaa atgcaagatt gcttttcgca acaatcgcat cacaataaga gtgtgccaag
     7321 atcgtgcgag aaagcacggt ggctcacaag gcgaggctat catgccgctt caaccttgaa
     7381 ggctagcccg ctggtgcctg cttgagctaa aagagtcgcc gaaagccaac gacctctgat
     7441 tcgcggtgtc acgatagggt cctacctatg agcaaccaca aaaacaagag ccaatggtcg
     7501 ctcgctttta tccttgaggt tgtcatcgga tgttgctgct tacttggctc aatgtcggtt
     7561 gcacggggcc aggtcattgt gcggcagtgg gagttcaatc ggtcaggtga cgcggaaggc
     7621 tggaaggctg gggggcatct cagcgacacc cacgtggccg acggcgtcct ccgaaccaag
     7681 gtcatcgatt gggacccgat tttggtccat gatgtctttg agccacctct tcctgcgacc
     7741 gcgacgcagg tgatcgaaat tcgcctattt gccccccatg cgggccaggc cgagttcttt
     7801 tggaccaaca cgacagaggg ccagtacggt ggttttgctc cgggaaagga gacccccttt
     7861 gccgtcaagc cagggtggaa tacctatcgg gtacagccat tttggcaggc ggaggggaaa
     7921 atcgtgcgat tgcggttcga tctcccttcg cgaacagcca atgaagaacc atcagagtat
     7981 cagattgatt acattcgtat cttggaattg gcgggtgagg gtcctccggg gcctgccgaa
     8041 tgggatttcg cacaggggct agcgggttgg caactagaag gggatggcaa actgtggtgg
     8101 gaaaatggct gcctgaacgc tgaggttcaa cccggggcaa ggttggtggc cccgccgatt
     8161 caagcggatg ccgatcaact agcttttgtc gccatggaaa tggccgtgga ccagccgggg
     8221 agtgcccgcc tgatttgggc ttctggaaag gtcaatgggc agcagtccac tggttttact
     8281 ttgcgggccg gggaacggtt cgccgtgtac aatctcccgg tcgcaggaac gtcgaactgg
     8341 aaaaccccgg tgatctactt ggctttggag ctgaccagca atcatcgtcc ggtaagattg
     8401 cgcctcaaat ggttgaaact cagtgaagag ccaggtgggc cgccggacct tgagttggaa
     8461 cgtttctttg tgaaggatgc ccttcccagg gtggacaagg tctgcaatgt tgtggctcat
     8521 gtcacaaatc gcgggggaga accagttcga gatgttcgcg ccgagcttat cctacccgat
     8581 ggagcggagt ttgttgattc ggcgtctccg ctaagacatt tggcggagtt ggaattcgga
     8641 gagacagaag ccctcgtttg gaaaatcaaa aatcgtcgag ttggccctgt ttctctgcgg
     8701 ctacgtatcg tttcgccaga actcgaagaa tacgagcgcc aggaagagtt tttgccagac
     8761 ctaagacttc cgcgagagga ttacgtgccg gagcccaagc ccgtgcgtgg gccatttgag
     8821 gtgggcgtgt actatttccc agggtggggt cggccaagct cgtggcaacc gatcgagcga
     8881 tttcctgaac ggcgtcccgt gctgggcttt tatcgggaag gcctgccgga aattgccgat
     8941 tggcacatca agtgggccgt ggagcatggg attacatttt tctgctacga ctggtactgg
     9001 aggcagggag agcgtcgatt agagcatgcc ctccatgatg gtttcttcca ggcacgatac
     9061 cgccacctga tgaagttctg cctcctttgg gcgaatcact ttgggcctgg tgaacacagt
     9121 gccgaggata atgagcgcgt ctgtaagtat tggatcgaca actacttccg tcggccagag
     9181 tatttcaaag ttgaaggtcg gccgttgttg gtgatcttct ccgttcactc gatgcagcgg
     9241 gacttgggga tcgaaggcac gcggaaagcc atcgagttat ggcaccgcat gacagaggaa
     9301 gccggggtgg gcaagattct ggtggccggt tgtgggaccc cggccaacct taagcagatg
     9361 aaagagatgg gcttcgatgc ggtgacgggc tacaactggc ccagttgtgg tattcaggac
     9421 cgaaattggg tcccttttag cgaagtggct gccaactacg acgcactgtg gtggcgcccg
     9481 ctggcggagg ccggagccat gcccgtgatt acccccgtca gcgcgggctg ggattctcgt
     9541 ccctggcatg gcgatcgggc tctggtcttg actgactgta ctccgcaagc atttcagcag
     9601 catttggagg atgccaagcg attcatcgaa gagacgggtc agcctcgtgt tcttctcatc
     9661 gaggcctgga atgagtttgg cgagggttcc tactgtgaac cgcacaagca gtacggtttc
     9721 ggtcatctag aggcaatccg caaggtcttc gccccggata gtcaaccccc ccttcattat
     9781 ggtccagaag atgttggccg accgctgttg gaattccgtg atctggaaaa gccggtttcg
     9841 cggacccaat ggaactttga cacaccggat caagatgaag gatggaaacc tctaatgggg
     9901 ctaacggatg tgacggtggc gaacggtgtt ctagccgccc ggacaatcag cggcgacccg
     9961 gcgtttcact gctcgacaaa actcagggcc aaggacttcg ccggtatcga aattacgatc
    10021 gccatgcggt catcgaagcc gaccgatatc gtgcaactct tttgggcaac gttgagcaat
    10081 acctatcggg aagaggccag tgtgagaacc accgtcgtct gcgacggtaa gatgcggacc
    10141 tatcgactgg acctggccgg ccatcctctg tggcgaggcg ttatcactgg ttttcggctc
    10201 gatgtcggtt cccaacccga tgcagagatc aagattgatt cgatccgcct gatccgcagc
    10261 gcgtccgagg cctttgagaa tctccgccgc gcgatcgatg atctgatcgc cacgttcggc
    10321 gcgtcctatc ctgagggcca agagtttcgc cgccgtttgg atgaattgga acaattgcac
    10381 agaaaaggac agccggtcga aaaggcgttg agcgagttac aaagaaaggc ccttttggcc
    10441 aatcccttgt tagattttga ccgcctcttg gtgttgaaac gcaagcgggg acagctcgga
    10501 ttgcccacca atcatcagtg caattcttgc ctggaccaga caggttacga taacgagctt
    10561 gcaatttttt catggaggga tggtactctc aagacgcttt accggcctcc ccagggtttt
    10621 tacgtcggtg aggttgacct tcattgggac gctgaccgcc tgctctttac aatgcccact
    10681 ggtcaaacct ggcagattta cgaaatgcgt ctcgatggga caggtctgcg gagggtctcc
    10741 cgagaagaac ctggggtcga taacttcgat gcctgttacc tgccggacgg cggaattgcg
    10801 tacgcctcga ccgccgcttt tacggcggtt ccctgttggc atgggcgaga gcgagcttgt
    10861 gcaatttttc ggatggaggc cgacggttca ggggttcggc aactttgttt cgatcaggac
    10921 ctcgatttgc acccggccgt cctgccaaat ggccaaatca ttttcagtcg ctgggactac
    10981 actggcatca tgcatattta ccttcgccca ttaatggtga tgaaccccga cggtacacag
    11041 caacgggcga tttacggatc gaactcctat tatcccaatg ccctttattt ccccagggcg
    11101 gtgccgggcc aagcctcaaa actcgtcgcc attttgtcag gttatcatgg ggtcaatcga
    11161 gccggggacc ttgttctgct tgattacggc cgtggttggc acgaggcaaa ggggattgtc
    11221 catcggttta tgcatgggcg agaaccgatc gagccgatca tccgcgatga cctcgttggg
    11281 ccgaactggc caaaattcct ccacccatat cccttgagcg aaaactacgt cctggtttct
    11341 gcccaattac accagaaagg tggctgggga atttacctgg tcgatcgctt cgataacatg
    11401 atcccgatcc ttgtcgatga gcaatacgat ttcttcgagc cgatccccat tcgcaagcag
    11461 ccacgccccc ctgtgattcc accccgggtc gatttgtctg atcccacggc ggtggtcttt
    11521 ctccaggatg tgtatcgtgg cccgggtttg aagggagtcc ctcgggggac gatcaagaac
    11581 ctgcggattg tcgcctatca ttggggattt cccgggatgg ctggcccgga caaggtaggg
    11641 cgagccggtc cgtgggatgt gatgcgaatc ttggggacgg tccctgtcga ggaggacggc
    11701 tcggcgctgt ttcgtatccc tgccaatact cccatcgcca tccaagccct cgatgaaaat
    11761 ggggcggcgg tgcagatcat gcgaagttgg tacacggcga tgccgggcga acaaacgtcc
    11821 tgtatcggat gtcatgagcc accgacggag gccccccagc aattgacccg actagtcgga
    11881 atgcgggaac cggtggacat caagccttgg tatgggccgc cgcgaggatt tgatttcgaa
    11941 cgagagatcc aaccggtttt ggacaaatat tgcgtgggct gtcacgacgg ccagggtaat
    12001 gatggcggga ggcttgatct tcgggccttg cggtttcacc ccgactatca agggttgccg
    12061 ctctcccaac tgggggccag ccgtctttgg ccggaggtgg cagcactttt gccttcgtgt
    12121 gagccaccac atcgcctcct gggtaacaaa cggtatccct acactccggc gtatgaggcc
    12181 cttattccct ttgtgcgtcg tgtcaatatt gaggacgatg tttacctctt gaatccttgc
    12241 gaatatagtg ccgatacgag tgaactaatt cagatgctga aaaaagggca ttacggtgtc
    12301 gaactcgacc ccgaagcttg ggaccgtctg caaacctgga ttgatctaaa tggtccctgt
    12361 catggtcgct ggggcgtggt ctgtcctgta ccgcaggagg ccaacaaaaa aaggttggcc
    12421 atcctgaaac agagtggtgg tcccgacatc gatcccgaag agttgccgga tattcggttc
    12481 gactctcagg tggcgaagat acctgagaga acgacaaggg aacgtccgcc gcgtccgcaa
    12541 ctggacacat ttccgctgtc accggatgcg gctttggccc gtcaacagga aagaccgtgg
    12601 acaatgacga tcacagtcag ccctgggatt gacctggaat tggtgcggat tccctcaggt
    12661 cggtttgtga tgggtgatcc aagcggcgat gatgatgaaa atcctgcctg cgtggtttcg
    12721 gtggaaaagg acttttggat cagccgttac gaaatcacca acgagcaata ccgctgcttt
    12781 gatcccgcgc atgactctag ggtgtttacc aagcggtcga taaacgtgaa tggaccgggc
    12841 gtggcgatga acgaggcaaa gcaacctgtg gttcgcgtct catgggaaga agcgatgtca
    12901 ttttgccgat ggttgagcga acggacgcat ttgcggtttc ggctgcccac cgaagcagaa
    12961 tgggaatacg cctgtcgggc ggggacagcc accgcgttct tctttgggga ccgggatagc
    13021 gatttctccc aatacgcaaa cctggcggat gccgccattg aacgcatcta cacaacgacg
    13081 ggtggcgtcg tcgtgttaca gccaattcct gcagatacca ggtgggatga cggtgctatt
    13141 gcgacgaagc aggttggatc atatcggcct aatgcgtggg gcctgtatga tatgcatgga
    13201 aacgccgccg aatggactct gagcctttat cgaccctatc catatcgtga agatgacgga
    13261 cgaaacgatc tcaccgtaag cgggtttagg gttgtccgcg gaggatcgtt ctatgatcgg
    13321 ccacaacggg cccgctccgc cttccggctc ttctatcgcc aatggcaaag ggttcacaac
    13381 gtgggctttc gcgtcgtgtg ccagattcag gaccaaagcg atctgcccgt ggcacttagg
    13441 agcctatctc aataagccct cttctccctc cgggagagag ttggggtgag ggcatgcgaa
    13501 cgtcagaatc aattgagaaa accggcatcg attcgagcca ccctcacccc cgacccctct
    13561 cccgccgaac cagcgaaggg agattatggg atacgctctt aatgcggcaa gtcaacccta
    13621 gctgtactcg gtggccaggg cgttgatcct tctccgctag ttggaagtcg agaggtgtca
    13681 agccgccgac cgcgtgcgtc cacaagacga tgcggacatg tcggtaggac tggagatgca
    13741 ggtcaggatg atgattttct acctcggcca attgtcccac ttccatgaaa aacctgagcg
    13801 cagtcgcgaa atccttcagg gtccactgtc gaaaaatcct tttgccgtcg gattccaact
    13861 cccaacccgg gagcccacgc aaacgcgcgt gcacttcctc tggcgttagg tgtgtggccg
    13921 acccttcaga ggatggctgt gcgcgcgaat cgtttgcggc gatctttttc cgacaatcgt
    13981 cttgtgccga catgtgattt cctcatcgta aatattccag gtaccaccgc gctgtttatc
    14041 ttgatttagg caggtaacct cggcttattg tattcagctc gaaaagcggt ctccccgtga
    14101 aaaagtctgt gtgcggcggc gatagccggc ggtgttgccg cctcctaaag acacgacaat
    14161 tcggtcccat ctcaaaactt cgtgctgtcg aaaaggtttc agcattgttc acagtgaggt
    14221 tggccgcaag aataagatag gaagaaccgt ctgagcgggg ttttccgttc taaatccgat
    14281 tgatgcagcg gcaaggggtg cctatctgat gcaggaatcg cgttctctta cgacgacgga
    14341 gatgatcctg gattctgtgg ctgacggtgt ttttaccgtg gaccatgaat ttctgatcac
    14401 cagcttcaac cgggcagccg aacagcttac cggtgtcccg cgctcagatg ccctgggccg
    14461 accttgctgc gaggtcttac gagcgagtat ttgtgagtca gcatgcgcat tgcggcagac
    14521 actggccacg ggacggccta ttgtcaataa agccgtcttc attttggata gtcacgggaa
    14581 tcgggtcccg atcagtattt caacagcagt gttgctggac gcgaatggta acgtcattgg
    14641 cggcgtagag acgtttcgcg atctctcatt tgccgaacaa ctctatcggg aacttgagag
    14701 cagcgaccgg tttgtcgata ttgtcggtcg ctctcgggcc atgcgtcaaa tcttccaact
    14761 cttgccccag attgcagaaa gccccagcac agtccttatt gaagggccga gcgggacggg
    14821 taaggaattg atagcacgtg ccattcaccg cctttcccca cgccggaata agccttttgt
    14881 ggcagtcaat tgttctgccc tgccagatac gctcttggag tcggaactct tcggctacaa
    14941 agcgggagcg ttcaccgacg ccaaacatga taagccgggg cggtttgcgc tggccgacgg
    15001 agggaccctg tttctcgacg aaattggtga catttctcca gcccttcagt cgcgacttct
    15061 gcgcgtcctt caagagcggg tcattgaacc gttaggcggc atcgaacccg tgcccgtgga
    15121 cgtgcgaatc attgccgcga cacacagacc gctggtcaag ctggtccgga agggcctctt
    15181 tagggaggat ttgttctatc gaatcaacgt tgttcgtatc gaattaccac cgctgcggga
    15241 gcggcgggag gatattccgc ttttgattga gtatttcgtt tccaagttta atcggctgca
    15301 cgggaagggg atcctgggcg tttcggatga agtccttgcc cgcttgatgg attatgacta
    15361 tccggggaac gtgcgggagt tggagaatat cattgaacat gccttcgtcc tttgtcgggg
    15421 ccatctcatt gagctggagc atttaccccc ctcggttcgc ggtgaaggag atcttcggga
    15481 ccgttcgcta acaaagggat tgacgctcaa ggaaatggag cgggttctca ttcgcgacgc
    15541 tttacgacgc catcaggggc accgcgtggc agcagcacga gagttgggaa ttcaccccag
    15601 cacactgttc cgcaagatga agagtttggg catcacatgg cccgagccag ggtgacagat
    15661 gtcattgcaa aatgcaatat atagtggcac gagaggttgc attttgcaat gatacaacct
    15721 tctcctcgga atcgttgtgt tgctgtaagt cttttcttat aaatgactta cgatatttct
    15781 ggatcacgct ttggtctttg gcgccgattt tgctccttca gataatcatg cgaattgcca
    15841 tttctgatca ccagggtcga gtgtcgcccg tattcgacgt cgcgactcat gtcacggtcg
    15901 tcgaatatga gcaggaaaaa gagatgagcc aggtcggcta cgacctggag atcgccgatc
    15961 cttaccggcg tgcagagttt ttagcgaatt tgcaaattga cgcgttgatt tgtggtgctg
    16021 tctcccggcc cttggaattg ctccttattg aaaaggggat tgccgtttat ggtcgagtgt
    16081 gtggggacgt tgcggaagtc ctgcgtgcgt tcatgttagg gaagcttacc ggcccaaatt
    16141 ttcggttacc tggttgccca gaggagggac cccgtcgttg gcggcgtggt tgtcgtcgtg
    16201 ggcgccacat gtcaaataat gaggaaacat catgaaggtt gttgtcacgg ctcagggaac
    16261 aacgttagat agccccgtcg atccacgctt tggacgggcg gccacgttcc tcctcgtcga
    16321 tacggagacg ctggaggcgg tcgcgcatga aaacacggca gccggtgaac cccagggggc
    16381 ggggacgcag gccgcgcggg cggtgatcat gcttgggcca caggcagtca tcacaggtca
    16441 tgtcggacca cgtgcgtttg ccgtcttcca agcggcagga ataccgattt ttctcgctga
    16501 gcgaggaacc gtccgtgagg cgattgcggc cttcaaagcg gggcaacttg cgctggccga
    16561 tcatcctaat gtgtgaccat ccagcgtgtt acgaaaacgc atgtccataa cacaggcggg
    16621 tttttacccg gtactttggt aagtttttga gtgaactgtt gaagaagaac tgtttaataa
    16681 cggaggtgaa atatgccagg aggagatcgt acaggaccga tgggcgctgg ccctcgaacc
    16741 ggtcgaggaa tgggctactg cgcaggttat ggtgttccgg gattcatgaa tcgacccggg
    16801 ctttggggtt ggtttaccgg ttggctaccg ggatgcggac gaggtggccg cggttttcgc
    16861 cattggtttt accgtaccgg cctgacaggt tggcaacggt ttgccatggg ttggcctgcc
    16921 tggggaggtg ttgttccgcc tactgtgcca ccgtctcctc agagcgaact ggatatcctc
    16981 aggcagcagg ccgagagcct gtctcaaatg cttgaacaga ttcagcgccg gatggaggag
    17041 cttgaaaaac gagggcagtc gggcgaaacc gcctaataac ccgggcaatg attcgcagtt
    17101 gagcgaatac gccgaaggct gcggtgcggc tagaatgggt ctggcaacag gaaagcggca
    17161 gttgcgttct ggtgctcact gttctccgca ccgcggcctt ggcgaacgcg ctttggccgt
    17221 ggaaatgtct gcaaaacagg cttgtgtcgc ggtgatattg cagctgcgtc cgaagtcgcg
    17281 gcggcacgcg cgaggagtgc ctcagtcttt ggccggcttc tatggcgatg cgcagacagt
    17341 gccttatcag gacgtcaaat attcatgaaa atcgttatcg ctagtggcaa aggtggt
//
LOCUS       DAOSUT010000083        17063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2082_length_17063_cov_9.299389, whole genome shotgun sequence.
ACCESSION   DAOSUT010000083 DAOSUT010000000
VERSION     DAOSUT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 17063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17063
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_17063_cov_9.299389"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PLD05_09100"
     CDS             complement(<1..1140)
                     /locus_tag="PLD05_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77638.1"
                     /translation="MVATLPASKYEVAQSEKGQEAFVTLEKRFQRLGADVKPVADEEI
                     YEVVRARLFEAIAPDSEPDYPKKVAKVYLEMYKAHSNEEILNRVKMISTQCGSKARLY
                     RNTLLFLVGTTRGLSKLRQAHREWAALEGVRRDYWDQLDEEQRNDLKKRLEDARKAAL
                     EALGPAYTVVLRVRGQEVESCALSDAGRTFQEHLSYLWKTLVEDEEWILRRVGSVTLG
                     STGLIPKEGGLSLKDAVEAFLRFTDKPMIASKEAVTEGLAQACREGLVGIGRGGSLSA
                     LQARYCKESVSLDPNEDGVWIIPPFVPEPAKVPEAEQTARASVQTQDSGMASKTISPT
                     TADSPKTTTATKPDTKGTVRRVVVRGHVPAEDWHELFHCFVKPAVR"
     gene            1327..1773
                     /locus_tag="PLD05_09105"
     CDS             1327..1773
                     /locus_tag="PLD05_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77639.1"
                     /translation="MSRSLLRRVFLFLQKTAITSAGEQGEEIQAELVRNLYRLWFSVA
                     MMAGITWWNLADGTAVQGENEARGGLMTEDLQPKPAYRELEQLIHQEWETRANGQTDQ
                     EGTYRVRGFHGRYLVRVGKESTWVVRTLTVDQKIPATLEVLWVENK"
     gene            complement(1977..2504)
                     /locus_tag="PLD05_09110"
     CDS             complement(1977..2504)
                     /locus_tag="PLD05_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOP77640.1"
                     /translation="MPNVLRMRTYAGAATIGSFFVVSLTGIIMFFHINIGWIKMAHEW
                     MSWVFVAAAVCHAVVHWRSLLGYFRNRAALAVIIPIVIIGLLSLSPVHGPASKHPLAQ
                     VMFRLEHASLESVATLTDRDVSEVVRRLKASGITIDTTNQTITEIATRNGRRPTEVLL
                     LLFSEEKPIKAVRHK"
     gene            3205..4272
                     /locus_tag="PLD05_09115"
     CDS             3205..4272
                     /locus_tag="PLD05_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77641.1"
                     /translation="MRVITSTELGSKKLGDDYINTARCMERDGFTLVELLVVIAIIGL
                     LIALLLPAVQAAREAARRAQCCNHLRQLGLAMQNYHNAYQMLPNTGFFGPTYPNDYSP
                     LAKLLPFVEQQNLHNLVDFRITLGHVGREDLPECLWSAARTPVSLFLCPSDGEKTIHD
                     LTLPSGRVIPVAGANYAMNQGSGLDGMFNPGFGATDGLCWVGAEIRLDDVLDGTSNTL
                     VFTESLRGPGTSLSASDRPNHQIYRARAVPTLIDVVEQQGLEALYPQVTGWDGTRLMF
                     WLRGCSPTGPVMNGRLLPNSRIPDLVGGSEKVTAARSWHPGGVNACFCDGSVRFIAQT
                     ISATSWHAFWTRAGGEVVSVP"
     gene            4302..5093
                     /locus_tag="PLD05_09120"
     CDS             4302..5093
                     /locus_tag="PLD05_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP77642.1"
                     /translation="MSDQGCRLSQRERKLVAENVTKSFRHVATTIQVLRGVSFSLLTG
                     EFVAIMGPSGSGKSTLLHVLAGLTPPDSGRVSLDGYEIGELSDREMTALRRRKIGIVF
                     QQFNLIPALTLEENVLLPLLAERVDRSRLEKARQIIRDLGLWERRHHRPDALSGGEQQ
                     RTAIARALAIDPTLILADEPTGNLDSIAGQQFCHLLKTLAKEQSRTILVVTHEPAVAI
                     WTDRVLILKDGQIVAEFPTESCNSVRSLAARYEESLQMGQLENTL"
     gene            5090..7999
                     /locus_tag="PLD05_09125"
     CDS             5090..7999
                     /locus_tag="PLD05_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77643.1"
                     /translation="MMPVSARLALADALHYPGRVAGASVALLAASCLVVWVVSAYDKL
                     VSEFEGTAQESFGHYDLFLLPDSLDEKAIVSEQLLRSLGNDSRIANIDRSLRGSVELK
                     EPSNLGERRNDRTAVVRGQRPGPRSPVPKNKLAPQPSVGPQQVRALVAFPTLVGIDAA
                     VPPYPLVAGRWLIPEDSGGDHAVISEQLAESRGWDVGQTITVTCDERTLALRVVGIVA
                     QPESVPTLEKTGPTGRPLGSAIGPALALPPGAIYVPLEVAQTFLGRPGQANFLCLKLR
                     KKRDVLPFLKDWRDQLKPFTDGMNYLAVDFQQMEKAFAAGRASHSIRSQAWTACGLTI
                     LAAAFLIFTTLNMGVDERSRYIGLCRIVGLTKSQMAWTVFIQAVILAILGWLGGLLSV
                     FLVLQLGHQVGLNIPWSGVIGPYTLGLSAVVTILGATIAALWPMLRAGRVPPLEALQP
                     RHANLLHSTNPLKLLPLAALGTVFIMIHPMVVFLLPLKDSFRVWIAITVGAPMAAIGF
                     LCWTPALVVAVEWICSPVLAFVMCVPRQLCKMELARNLPRSVATCMALSVGLGVFSAM
                     MVWGYSMLEPFKPTDWAPDMLAAFQSGSVPKQELQTIASIPGVKRYLPAAVEQPKLAE
                     DLTHASEGASVTRQDNVIIMGVDPDLAFGEDDPLFHLDFVKGSRREIIRRLKSERACI
                     VPDHFVTATGLDIGDTFRVVSPNDPQKTLEYKIVGVVSLPGWHWMTKFSGLRRRYARS
                     AALIFAEESVVRFDFDLKNVNFIWIDLAKNADLEVVCRKLQDVADRHLGEVQPVNIQG
                     TWSFGARMFGRSLRVTLRDEIYQRVTQRADTMIWAMARLPFVTLLITLPGLMSVINAS
                     VRIRQWEFGIMRAIGFTSSLIARQIVVEGLLLATVAAFVSVLFGIVSGWCGIGLSQYT
                     SFFGGMQTPLVIPWRQIAIGVVATFLICGIAAVPGAIVVLRREPLKLFLAGRGSE"
     gene            8613..10994
                     /gene="cas3"
                     /locus_tag="PLD05_09130"
     CDS             8613..10994
                     /gene="cas3"
                     /locus_tag="PLD05_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOP77644.1"
                     /translation="MQNRCFWAHSANDGGKGVPERLRDHLERVALRAKEFASSFEAGE
                     QAQLAGILHDLGKYADKFQQYLEGKVQRAGDHWSAGSLLALKLANKYGECPALAVLAH
                     HSGLNELPGSYKQFWNDLCSRIRRGDEVTESHLDKLWQRLCAEGVPVPTVSSGLVLQR
                     QHLVADMLDVRMLLSALVDADYLETAAHFDGDSETPYRVPPAGPVLETDKAIEALEIY
                     LEELRVSRKEDPLNPLRDRLQGQCRRTAQQPQGLYTLSAPTGLGKTIAMLIFALHHAR
                     KHNLRRIILVMPYINIIDQTAQIYRKIFSVENGFPENFVLEHHSLKDDSSDESNEQDS
                     TQRLLAENWDAPIILTTNVQFFESLFANRPSRCRKLHRIARSVVLFDEVQTLPVSLAV
                     VTLAVLSRLADQTGPYHCTCVFATATQPAFTVLHERVKEYAPAGWQPVEIVSNPQELF
                     QATASRVVTTWRHEHVVTMKELAEELAGHSQVLCIVNLKRHAVELFRHLSQYLPENER
                     SSVLHLSTALCPQHRRNVLNEVNRRLTDEAPLRLIATQCVEAGVDLDFPVVYRAVAPL
                     EAIAQASGRCNRHGRRHVGNLIVFRPADDRDPYPPGYGHAAEATCYYLRQLARDRPEK
                     LPSLLADPDGIREYFEVFYSMQGLADRSGILYEERELERAIRAGNFEMVATYYRLISQ
                     QTIHILVPYEEERFKKLLEEIEQGRGKPGFIRRWCRQAASFTVDIFPPKKDSPSWYHL
                     NPIEFFHSRQPTQGGTDWYYLLPGVTDVSQTLHGLAYHPQLGLVLPEGEICVV"
     gene            11021..11740
                     /gene="cas5c"
                     /locus_tag="PLD05_09135"
     CDS             11021..11740
                     /gene="cas5c"
                     /locus_tag="PLD05_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOP77645.1"
                     /translation="MTHVQQHTLEVWGDFACFTRPEMKVERYSYPCPTPSAVRGIFDA
                     IYFKPQFYWQPDRVELLTYPSYLPLRRNEVKEKINVASVKKWMSGSEPPEPILADADR
                     SITGTDQKGRTQRQTLALRKPRYRITAHIVPRPGYEKEQAACDAQFVRRASQGKCFHQ
                     PALGCREFVAFFRYVSQLDQEPPAVDYNQDLGWMLYDVFDIRTANGQDGSPCISLFRA
                     QIVHGVLDIPPYESELVRKPK"
     gene            11765..13621
                     /gene="cas8c"
                     /locus_tag="PLD05_09140"
     CDS             11765..13621
                     /gene="cas8c"
                     /locus_tag="PLD05_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOP77646.1"
                     /translation="MLHHLYNYAVREGIILPPGFQSRAIKWLLVFSPDGTFIEAVDLS
                     GGQKKSKGREFPRVPQAPANWLQGGGRSNFLVESLATAVNWPDDEENRHKIQQKHDFF
                     VALLLQVAQATPSHWALPEVLPLAQSLQNNEVLTTIQGHLVEKKAKPTDIATLAITTT
                     KAVPRIFVEETSWHNWWQDFLIKIKQPEMPTGSGPRRRKQEIEQMCDFLTGELVQPAR
                     THPKVKGLADIGGLATGDVLIGFDKDAFSSFGLQQSTNAAMSEESATIYVTALNHIVA
                     RRCYRVAKPKQGSGVKIGYWYSGAVPEELDPALDILGELAETTVPNQHESHSSLEVDQ
                     AEMRARRALRALRDGERPDLGNFHYYAMTLSASSGRVIVRDWMEGNFRELVENVNAWF
                     DDLMIVSRDGKKLVQTFKFADLLGATVRELSEVTAPLAAAMWRAALKRMPIPFEVVAR
                     TLRRIEIDIIEDETPRHSRFALLKAFTIRCERVPAMTAELNEHETHPAYLCGRIMALL
                     GAIQREALGDVGAGVIQRYYAAASATPALVLGRLIRTAQIAHLPKIQEEGLRYWFENQ
                     LAELWTKMRSVPPRVLTLEEQTLFAMGYYHQMAARFKKTSQGGNESELAQTK"
     gene            13731..14705
                     /gene="cas7c"
                     /locus_tag="PLD05_09145"
     CDS             13731..14705
                     /gene="cas7c"
                     /locus_tag="PLD05_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOP77647.1"
                     /translation="MSQPITSRYEFLYLFDCENGNPNGDPDAGNAPRIDPEDLHGLVS
                     DVAIKRRIRNYVQIAKGNQMPYAIFVEQACNLNKHIARAHEETNGKLPDTKGAKKEEV
                     EKAREWMCKNFYDVRTFGAVMSTGPNAGQVRGPVQVTFARSLDPVLPLDISITRMAVA
                     DDVSGAKSWRDYEKWEQEQKEDTLRTMGRKALIPYGLYLGKGFISAHLAEQTGFSTDD
                     LRLLFEAILGMFEHDRSASKGHMTVRPPVFVLKHVGTDTDPQQRKRQAMLGCAPAHVL
                     FEEIIEVKRKDGVTVPRSFEHYQVVVHNDRVPKGTELLRLPEDMDKLT"
     gene            14832..15467
                     /gene="cas4"
                     /locus_tag="PLD05_09150"
     CDS             14832..15467
                     /gene="cas4"
                     /locus_tag="PLD05_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOP77648.1"
                     /translation="MFTDDELVPISALAQYVYCPRRAALLFLERQWADNVFTVEGRHV
                     HQRAHRTNLRERLPDGYAIHNLLLKSERLGLVGTADVVEFHEKDPAGPRVTVVEYKRG
                     KHKRSKQTEYEVQLCAQVICLEEVLNIQISRGTLFFAKSKHRYDVECHRTLRTIVEDT
                     TQKVRQLFQGTCTPAPQYGRKCRGCSVIDICFPKVVSRKNVQYYIKHIVQE"
     gene            15506..16537
                     /gene="cas1c"
                     /locus_tag="PLD05_09155"
     CDS             15506..16537
                     /gene="cas1c"
                     /locus_tag="PLD05_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOP77649.1"
                     /translation="MKHHLNTLFVTTQGTYLRKSGEAVAVCIEKQIRLRIPLINLGGI
                     VCFGRVSCSPALLAACARAGISVSFFTEFGRFLAVCQGFTSGNVLLRRTQYRWADDPE
                     KTAILARMFVMGKIANTRSVLRRAAREHEDPAAIRDISQIADQLTQILSMLEHAGGVE
                     LVRGLEGQASAAYFSVFNHLILTQKDHLKFTCRSRRPPRDPVNALLSFLYSLLVADVR
                     GACEASGLDAGVGFLHRDRPGRPGLALDLMEEFRAILADRVALTLINRQQIKPKDFIN
                     QPGGTVVLTDSGRRRVLEEYQARKNETITHPFLNEKMTLGLVPHIQARLLARVLRGDL
                     DTYPPFVLK"
     gene            16550..16843
                     /gene="cas2"
                     /locus_tag="PLD05_09160"
     CDS             16550..16843
                     /gene="cas2"
                     /locus_tag="PLD05_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOP77650.1"
                     /translation="MLVVVTYDVSTKDKAGQRRLTRVAKKCLDYGQRVQNSVFECVVT
                     PEQWVTLRKELLDLIDPEEDSLRFYFLGSRNRHRVEHVGAKPSLDLEEGFLIG"
BASE COUNT         3946 a         4449 c         4592 g         4076 t
ORIGIN      
        1 gcgcacggca ggcttgacaa agcaatggaa gagttcatgc cagtcctcgg ctggaacgtg
       61 accgcggaca acgactcgcc ggacggtgcc tttggtatcg ggcttcgttg ccgtggtcgt
      121 tttcggactg tctgcggtag ttggggaaat tgtcttactt gccatccccg agtcttgggt
      181 ttgaacactt gcccgggcag tctgttccgc ttctgggact ttcgctggtt ctggtacaaa
      241 tggcgggata atccacacac cgtcttcgtt cggatcaagc gagactgatt ccttacaata
      301 acgtgcttgc agcgcagaaa ggcttccgcc ccggccgatg ccaacaagtc cctcacgaca
      361 ggcctgagcg agcccctccg tcaccgcctc cttcgatgca atcatcggct tgtctgtgaa
      421 tcgaaggaaa gcctcaacgg catccttcag cgatagcccg ccttccttcg ggatcaagcc
      481 tgtacttccc agcgttactg agccaacgcg ccgaagaatc cactcctcgt cctcgacaag
      541 cgtcttccag agatagctaa ggtgttcctg gaaggtccgg ccggcatccg agagcgcaca
      601 cgactccact tcctgtccgc ggactcgaag gaccacagta taggcgggac ccaatgcctc
      661 cagcgccgcc ttgcgcgcat cctccagacg cttcttgaga tcatttcgct gttcctcgtc
      721 aagctgatcc cagtagtccc tgcggacacc ctccagagcc gcccactctc ggtgtgcctg
      781 ccgcagtttg ctcagtcccc gagtggttcc caccaggaat agcagtgtgt tgcggtacag
      841 gcgggccttg ctgccgcatt gggtgctgat catcttcacg cgattgagga tctcctcgtt
      901 agagtgggcc ttgtacatct cgagataaac tttggccacc ttcttcggat aatctggttc
      961 gctatcgggt gcgatagcct cgaagagccg cgcccgaacc acctcgtaga tctcctcgtc
     1021 cgcgaccggc ttgacgtccg ccccgagacg ctggaagcgc ttctccagcg ttacaaaagc
     1081 ctcctgtccc ttctcgctct gagcgacctc atacttgctc gccggtagag tggcgaccac
     1141 cggatcggtt tctcccgtgg gcagaaaagt atggcatcac cctcaaggga catcctctgc
     1201 tttggcatgc acataaccca tcgtggttgc caaaagatcc agaagccctc aaggccctgt
     1261 atcgaaaacg gttcaaagaa atcgcagaac gctatgctgg caaaatcaaa gtgtgggatg
     1321 tcgtgaatga gtcgctcgtt gcttcgccga gttttcctct ttttacagaa gaccgcgatt
     1381 acgtccgctg gtgaacaggg cgaggagatc caagcggaac tggtgcgaaa tctttatcgg
     1441 ctatggttta gcgtggcaat gatggccggg atcacctggt ggaatctcgc cgatggcacc
     1501 gccgtgcagg gagaaaatga agctcgcggc ggcttgatga cggaggactt gcagccaaaa
     1561 ccggcctacc gggagcttga acagcttatt caccaggagt gggaaacacg ggcaaacggc
     1621 caaaccgacc aagagggcac ctatcgtgtg cgggggttcc atgggcggta cttggttcgt
     1681 gtggggaagg aaagcacttg ggtcgtgcgg acgttgacag tcgatcaaaa gatacccgca
     1741 acactggaag tcctatgggt ggaaaacaag taactggccg gtttgtcaaa cgaatggaca
     1801 acggagccac gcaagacgcg accaggagta aacgcgacgg attattatgg cttgcgagat
     1861 atgaaaactc ttttgaggaa aattagacgc ctcggttttc ctctattgtg gcctcatccg
     1921 tgtgataaag atataaagag acaattcttc gaactcgcca tatagcggga ccggtatcat
     1981 ttatgccgta cggctttaat gggtttttct tctgaaaata gtagaagcaa gacttccgtt
     2041 ggccgtctgc catttcttgt ggcaatttcc gtaattgtct ggttggtggt gtcgatcgta
     2101 attcccgacg ctttcaagcg acgtactacc tcggatacat cccgatccgt cagcgtcgcc
     2161 actgactcga gggatgcgtg ctccaaacgg aacatcactt gagccagcgg gtgtttcgag
     2221 gctgggccgt gcacgggaga cagagaaagc aaacctatga tgacgatagg aataatcacg
     2281 gcgagtgccg cccggttgcg aaagtaccct agaagcgatc tccaatgaac cacggcgtga
     2341 catacggctg cggccacgaa gacccagctc atccactcgt gggccatctt gatccatccg
     2401 atattgatgt ggaagaacat gatgatgcct gtcagggata cgacaaaaaa cgagccaatg
     2461 gttgctgcac cagcatacgt tcgcatccgt aagacgttag gcatggtatc aagttccttt
     2521 gtccaaaaaa atccggcaat tcctttatcc aacgtattca agaaattgac tttacatgac
     2581 ttgcctgcaa tcgctgcacc gttatgctgg gcaacgggtc aaccgatgga gtcggatcaa
     2641 tactactaat gcaagtctcg tgccacacgt ctgaagaatc cattcggcat aggatgatgc
     2701 ccagcagccc gcctagtttg gcgttcccca atgtgtgtct gagaccgatc ccacaagtat
     2761 gaacacccaa gagtcctcgt tggtttcggg accgctcctc agggagagta ttttgtctag
     2821 gagttcgaca gtcgccaacg cagttagcag gtttgccttt attgactaga ggagctccac
     2881 aatagctcag tctggtgtct tggcgaagta cgttgatgtg tccagcaact cctccgataa
     2941 aactgccagt caggaaatag gtgtcaagta tctggtgggt tgtttctttt cggcgtgggt
     3001 aattatgtcg ttgtgcgcaa catacagtgt cgcgcgaacc aacaagacac tgatgaggtt
     3061 tttctgggaa cttcgtttgc ggagcgatga aaacagcctt cgaacgctat tggagcgtag
     3121 tttgagctgt ggcatccgaa ttgcatgagc attcgtatga caaactttta ccccggccag
     3181 ggacggaatc tcgggagaca ctgtatgaga gtgatcactt ccaccgagct cggatcgaaa
     3241 aaactggggg atgattatat caacaccgcc cggtgcatgg agcgagacgg cttcaccttg
     3301 gtggaattgc tcgtagtcat tgccatcatt gggttgctga tagcacttct tcttccggca
     3361 gttcaagcag ctcgtgaagc tgctcgccgt gcccaatgct gtaaccattt acggcaattg
     3421 gggctagcga tgcaaaacta tcataacgcc tatcaaatgt tgccgaatac aggctttttt
     3481 ggtcccacat acccaaatga ctattcaccg ttggcgaagc ttcttccatt cgttgagcag
     3541 caaaatcttc ataacttggt cgatttccga attacattgg gacatgtagg ccgggaagat
     3601 cttccagaat gcctctggtc cgccgcccga actccggttt ctctcttcct ttgtccgagt
     3661 gacggcgaaa agacgattca tgatctaacg ttgcctagtg gtcgtgtcat tcccgtggcc
     3721 ggggcaaact atgctatgaa tcagggcagt ggcctagacg gtatgtttaa tccaggcttt
     3781 ggtgccacgg atggtctttg ttgggtggga gccgaaatcc gactggacga tgttcttgac
     3841 ggcacgagta acactcttgt gttcactgaa tcattgcgcg ggccaggaac atccttatcg
     3901 gccagtgacc ggcccaatca tcaaatttac cgagctagag ctgttcccac gttgattgac
     3961 gtggttgagc agcagggttt ggaagcgttg tatccgcagg taacgggttg ggatgggact
     4021 cgactaatgt tttggctgcg cggatgctct ccaactggtc ccgtcatgaa cgggcggttg
     4081 ttacccaact cccgcatccc agatctggtt ggtggttccg aaaaagtgac agcagccagg
     4141 agttggcatc ctggtggggt taacgcctgt ttctgtgacg gaagcgtacg ctttatcgcc
     4201 caaaccattt cagcaacctc ttggcatgcc ttttggactc gtgctggcgg tgaagtggtg
     4261 agtgtaccct aacatgcggc ttttccggca aaggaattga aatgagtgat caggggtgtc
     4321 gtctgagtca gagagaacgc aagcttgttg cggagaacgt aacgaagagc ttccggcacg
     4381 tcgccacgac gattcaggtt ctccgtggtg tctctttttc gcttttgacg ggggaatttg
     4441 tggccatcat ggggccaagt ggttcgggca aaagcacgtt attgcatgtt cttgctggac
     4501 tcacaccacc ggattcaggg cgtgttagct tggacggtta cgaaatcggc gagctttcgg
     4561 atcgagaaat gacagccctt cgacgtcgca agattgggat cgtttttcaa caatttaatc
     4621 tgattcccgc gctgactttg gaagagaatg tgcttttgcc ccttctggcg gagcgggttg
     4681 accggagtag gttggagaaa gcgcgtcaga tcattcgtga cttggggctt tgggagcggc
     4741 gccatcatcg acccgatgct ttgagtggcg gcgaacaaca gcggacagcg atcgcaagag
     4801 ccctggctat tgacccgacc cttatcctag ccgatgaacc aacgggaaac cttgattcaa
     4861 tagccggtca acagttctgt catctgctta agacgctcgc gaaagaacag tcccgcacca
     4921 tcctggtggt gacgcatgag ccagccgtgg caatctggac agaccgtgtc ttgattctga
     4981 aagacggcca aatcgttgcc gagtttccta cagaaagctg caattccgtg cgctctctgg
     5041 cagcacgata tgaagagagc cttcagatgg gacaactgga aaacacgcta tgatgcccgt
     5101 cagtgcaaga cttgctttgg ccgacgcgtt gcactacccc ggacgtgttg cgggagcatc
     5161 ggtcgccctt ttagccgcgt cctgtttggt ggtatgggtc gtatcggcat acgacaaact
     5221 tgtgtccgag tttgaaggaa cagcgcaaga atcattcgga cattatgatc tgtttcttct
     5281 accagattcc ttggacgaaa aggccattgt ttcagaacaa cttctcagat ctctaggaaa
     5341 tgattcgcgg atcgccaata ttgatcggtc gttgcgagga tctgtggagc tcaaggaacc
     5401 gtcgaatctt ggtgaacggc ggaacgatag gactgcggtt gttagggggc agaggcccgg
     5461 tccccgctcg cctgttccga aaaacaaact ggcaccacag ccctccgttg gtccgcaaca
     5521 agtgcgtgcc ttagtcgcat ttcccacact ggttggtatc gacgcagcgg tgccccctta
     5581 tccgttggtg gcggggcggt ggttgatccc cgaagattcc gggggtgatc acgccgtcat
     5641 ttcggaacag ttagctgaaa gccgagggtg ggacgtcggc caaacgatta ctgtaacatg
     5701 cgacgaaagg actttagcgc ttcgggtggt agggatcgtg gctcaacctg aaagtgttcc
     5761 cacactggaa aaaacggggc caacgggtcg gcctttgggg agtgcgattg gtccggcctt
     5821 ggcactgccg cccggggcaa tctatgtccc tttggaagtg gcccagacat ttcttgggcg
     5881 gccgggacaa gccaatttct tatgtctgaa attgagaaag aaaagagacg tcttaccatt
     5941 tctaaaggac tggcgtgatc aattgaaacc ctttacggac gggatgaatt atcttgctgt
     6001 ggacttccaa cagatggaaa aagcctttgc cgcggggcga gccagccatt ccattcgctc
     6061 tcaggcgtgg acagcctgcg gtctaacaat cttggctgcc gcgtttctca ttttcaccac
     6121 actcaacatg ggggtggacg aacgttcacg ttacataggt ctttgccgga ttgtgggctt
     6181 gaccaagagt cagatggcgt ggacggtttt cattcaggcc gttattttag cgatcctcgg
     6241 ctggttgggt ggactcttgt cagttttctt ggtgcttcaa ctcgggcacc aagttggtct
     6301 caatatccca tggagtggtg tcattggtcc ttacaccctt ggcctctcgg cggtggttac
     6361 cattcttggg gccacgatcg cggccctctg gccaatgttg cgtgcaggtc gggtcccgcc
     6421 actggaggcc ctacaaccga ggcacgccaa tctgctccat tcaacgaacc ctttgaaact
     6481 cctccccctc gccgcattgg ggaccgtttt catcatgata caccctatgg tcgtttttct
     6541 tttgccatta aaggactcct tccgagtctg gattgccata accgtagggg caccgatggc
     6601 ggcgattggg tttctctgtt ggacacccgc cctagtcgtc gccgtggagt ggatatgctc
     6661 gcccgtttta gcatttgtca tgtgtgttcc tcggcaactg tgcaagatgg aactcgcacg
     6721 aaacttgcca cgaagtgtgg ccacgtgcat ggcgctctcc gtgggattgg gagtgttctc
     6781 ggccatgatg gtttggggct actcaatgtt agaacctttc aaacccacgg attgggcacc
     6841 agatatgctc gcggcgtttc agtccgggag tgtgccaaaa caggaactac aaacaatcgc
     6901 ttccattcct ggggtgaagc ggtatcttcc tgccgcggtt gaacaaccta agcttgcaga
     6961 agaccttacc cacgcatcgg agggcgcatc tgttactcgt caggacaatg tcatcattat
     7021 gggtgtcgat ccagatttgg cttttggcga ggacgatccg ctctttcatc tcgactttgt
     7081 taagggttca cgaagggaaa tcatccggcg ccttaagtca gagcgggctt gcattgtgcc
     7141 tgatcatttt gtgacagcca cgggattgga cataggggac acttttcggg tagtatcacc
     7201 taacgatccc cagaagacac tagagtacaa aattgtcggc gttgtttctc tgcctggatg
     7261 gcactggatg accaagtttt ccgggcttcg aagacgatat gcgcgttctg cggctctcat
     7321 ctttgccgaa gagagtgttg tccgctttga tttcgaccta aagaatgtta acttcatttg
     7381 gattgatcta gcaaaaaatg ccgacctcga agtggtttgt cgtaagctcc aggatgtggc
     7441 cgatcgtcat ttgggggagg tccaaccggt taacattcaa gggacatggt cttttggtgc
     7501 tcgcatgttt ggccgttctt tacgggttac actgcgggat gagatctacc agcgcgtgac
     7561 gcaacgggcc gatacgatga tttgggccat ggctcgactt ccgttcgtga ccttgctcat
     7621 taccctccct gggcttatga gtgtgatcaa tgcctcggtg cgaatccgac agtgggaatt
     7681 tggtatcatg agggccattg gtttcacttc gagtctcatc gcccggcaga tagttgtgga
     7741 aggtctcctg ctcgccactg tggcggcctt cgtcagcgtt cttttcggaa tcgtatcagg
     7801 gtggtgtgga atcggtttat cgcagtacac cagtttcttc ggcggtatgc agacgcccct
     7861 cgtcattccc tggcgtcaga tagctatcgg ggtcgtggcg accttcttga tctgcggtat
     7921 tgcggccgtt cccggggcga tcgtcgtcct ccggcgagag cctctcaaac tctttctagc
     7981 tggacgtggt agtgagtaag aagcggcgcc tcgtgaatcg tttgccaagc ctttcaccgg
     8041 agttcgattg acatcttgtt ggttgcagac gtcgttggaa cactgcgcct gttgaatgtg
     8101 gtgtaagtcc agatttgtta cctgctggag gtcatccaga caaccgcggc acggctttct
     8161 tatcctcccc caagcatttg gccgtttctc ggaacgatcc cgtcgctggc aattggcagt
     8221 gccaattcga tgtcggtgaa aacgccactc tgggcaatct gctattccac ctatcacgtt
     8281 ttggcacaca agatccccgc ctcttggacc gccaagggat gtcccccaca ctatgcaaaa
     8341 tgaaacgccg tgaaatcacc atggaaatga tgctgatagg tgcgcgcact ctccccccag
     8401 aagattctgc ggggctgatt gtccgatgga gtgtgaacag aagacgatgg tcttgtctat
     8461 gacgagacta cccggaggag tccgaatgtt cttacgctct tgatgagaca aaacacgacg
     8521 ttacattgct tcatgagttg ggagttctcg atattctcgg atcatcggtg ttagcaagtc
     8581 attgttgcca acctgtttta gtgagggcga atatgcagaa tcgttgtttt tgggcacaca
     8641 gtgctaatga cggtggcaag ggtgtccctg agcgattgcg agaccatctc gaacgtgttg
     8701 cgctccgcgc gaaagagttt gctagctctt ttgaagcggg tgaacaagcc caacttgccg
     8761 gcatattgca cgacttgggc aaatacgcag acaagtttca acaatatctg gaaggtaagg
     8821 ttcagcgtgc tggtgaccac tggtcggcag gaagcctcct cgcccttaag ctggccaata
     8881 agtacggtga gtgcccagcc ctagcggtcc tagctcatca cagcgggctg aatgaattgc
     8941 cagggagcta caagcagttc tggaatgatc tttgtagtcg catacggcgg ggtgatgagg
     9001 taacggaatc tcaccttgac aaattgtggc aacggctttg tgctgaagga gtcccagtac
     9061 ccaccgtcag cagtggcctc gttctccaga ggcagcacct cgtagcggac atgctcgacg
     9121 tgcggatgct gctttcggct ctggtagatg ctgactatct tgaaactgca gcccatttcg
     9181 acggtgattc agaaacgccc tatcgggttc cacctgccgg tccggtactc gagactgata
     9241 aggcgattga ggctctggag atttatctcg aagaattacg ggtatcgcgg aaggaagacc
     9301 ccctgaatcc gcttcgtgac cggttacaag ggcagtgccg ccgaaccgcc cagcaaccgc
     9361 agggactcta cacgctttcg gcgcccacag ggctaggcaa aaccatcgcc atgctcatct
     9421 ttgccctcca tcacgcgagg aagcacaacc tccgccgtat tattctcgtg atgccatata
     9481 tcaacatcat tgaccagaca gcccaaattt atcgtaagat cttctctgtg gagaacggat
     9541 ttccagagaa cttcgttctt gagcaccata gtctgaaaga tgattcttca gatgagagta
     9601 atgaacaaga ctcgactcaa cgattgctcg cggaaaactg ggatgccccg attatcttaa
     9661 cgacaaacgt ccagtttttt gagtcgctct tcgccaaccg accgtcacgc tgccgtaagc
     9721 ttcatcgaat tgctaggagt gttgtccttt tcgacgaggt tcaaacattg cccgtttctc
     9781 tcgctgtcgt gacactcgcc gtcttatctc ggttagctga tcagacggga ccgtatcatt
     9841 gcacgtgtgt atttgcaacg gcaacgcaac cggccttcac tgtgcttcat gagcgtgtga
     9901 aggaatatgc acctgccggc tggcaacctg tggagatagt gtcaaatccc caagaactct
     9961 ttcaggcgac cgcgagccgt gtcgttacca catggcgaca cgaacacgtt gttaccatga
    10021 aagagcttgc ggaggagctt gccggtcact cgcaggtctt gtgtatcgta aatctcaaac
    10081 gtcacgcggt agagctgttt cggcacctca gtcagtacct gcccgaaaat gagcgttcct
    10141 cagtgctcca tctctcgaca gcactctgcc cacaacatcg caggaatgtc ttgaatgaag
    10201 tgaaccgccg tcttaccgac gaagcacctc ttcgactgat tgccacacag tgcgtcgaag
    10261 ctggtgtcga tttggatttc cccgttgtct atcgcgcggt agctcccctt gaagccattg
    10321 cccaagcatc agggcgatgc aatcgtcatg gacggcgcca tgtgggtaat cttatcgttt
    10381 ttcgacctgc cgacgataga gacccttatc caccaggata cgggcacgcg gctgaggcga
    10441 cctgctacta tcttaggcaa cttgccaggg atcgcccgga gaaactgcca tccctcctcg
    10501 cagatcccga tggcattcgg gagtactttg aggtatttta ttcgatgcag gggcttgctg
    10561 accgaagtgg gattttgtac gaagaaaggg agctagaaag ggcaatccgg gcaggaaatt
    10621 tcgaaatggt ggcaacgtac tacagactta tctctcagca aaccattcat atactcgttc
    10681 cttacgagga ggagcgtttc aaaaaactcc tggaggagat tgaacaggga agggggaagc
    10741 caggttttat tcgccgctgg tgccggcaag ccgcatcgtt cacggtggat atctttccgc
    10801 ccaaaaagga ctcccccagt tggtatcatc tcaatcctat agagtttttt cattccagac
    10861 agccgacgca gggaggaaca gactggtact atctgcttcc cggcgtcact gacgtctccc
    10921 aaacgcttca cggcttagcc taccatcccc agttaggttt ggtgcttcct gagggtgaaa
    10981 tctgtgttgt ttagcggtca ttgaaaaagg agattctcgt atgacacatg ttcaacagca
    11041 cactttagag gtgtggggcg atttcgcctg ttttacgcgt cccgaaatga aagtcgagcg
    11101 ctactcctat ccttgtccca cgccaagtgc cgttcgcggg atttttgatg ctatctactt
    11161 caagccacag ttctactggc aacctgaccg cgttgaattg ctgacgtatc ccagctacct
    11221 tccacttcgc aggaatgaag tcaaagagaa gatcaatgtt gcttctgtga aaaaatggat
    11281 gtctggctcc gagccacccg agcctatcct cgcggacgct gacaggtcta ttacgggaac
    11341 agaccaaaag ggtagaactc aacggcagac tctggcactc cgcaaaccgc gataccgtat
    11401 cacagcccac attgttccgc ggcccggtta cgaaaaggaa caggcagcct gcgacgcgca
    11461 atttgtgcgt cgggcatccc agggtaagtg ttttcaccag cccgccctgg gatgccgcga
    11521 gtttgttgca tttttccgct acgtttcgca acttgaccaa gaacctccgg cagtggacta
    11581 taaccaagac ctgggttgga tgctctacga cgtttttgac attcgaacgg ccaacgggca
    11641 ggatggatca ccctgcattt ctcttttccg tgcccagatc gttcatggtg tacttgacat
    11701 tccgccttat gaaagtgaat tggtccgaaa acctaagtag gttccgcttt aggaggcatc
    11761 gagtatgttg caccatctct acaactatgc cgtgcgggaa gggatcatac tgcccccggg
    11821 gtttcagagc agagccatca aatggcttct cgtcttttct ccagacggga cattcattga
    11881 agctgtggac ctgagcgggg gccagaaaaa aagcaaggga cgagaattcc cccgggttcc
    11941 ccaggcacct gctaactggc ttcaaggagg cgggcgttca aacttcctgg tggaaagctt
    12001 ggccaccgct gtcaattggc ccgatgatga agaaaaccgc cacaaaatcc aacaaaagca
    12061 cgattttttc gtcgcactgc ttctccaggt tgcccaggca acaccctccc actgggcctt
    12121 gcccgaggtt ctccctctgg ctcagtcgct tcagaacaat gaggttttga caacgattca
    12181 aggacacctc gtcgaaaaga aggcaaaacc gacggacatt gccaccctag cgatcaccac
    12241 gacaaaggca gttccgcgaa tcttcgtaga agaaacttcc tggcacaatt ggtggcagga
    12301 tttcctcatc aaaatcaagc agccagaaat gccgactgga agcggtccgc gacggcgcaa
    12361 gcaggaaatt gagcagatgt gtgactttct cacgggcgaa cttgttcaac cggcacgcac
    12421 ccaccctaag gtcaaagggc tcgccgacat cggggggctg gcaacaggtg atgtcctgat
    12481 aggtttcgac aaggatgcct tcagctcctt cgggcttcag caaagtacta acgctgcaat
    12541 gagtgaagaa agtgcaacaa tctatgtcac tgctttgaat catatcgttg ctcgccgttg
    12601 ttatcgtgtg gctaagccca agcagggttc tggggtcaaa attgggtatt ggtatagcgg
    12661 tgcagtccca gaagaacttg atcctgccct ggacatcctg ggcgaactgg cggaaacgac
    12721 agttccaaac caacacgaaa gtcattcttc cctcgaagtt gatcaagcag agatgcgagc
    12781 ccgcagagca ctccgagcat tacgcgacgg cgagaggccc gaccttggca attttcacta
    12841 ctacgccatg actctctctg ccagtagtgg acgagtgatc gttcgcgatt ggatggaggg
    12901 aaacttccgc gagctagtgg agaacgtcaa cgcttggttt gacgatctca tgattgtttc
    12961 tcgagacgga aaaaagcttg tgcaaacatt caagtttgcc gatcttctcg gggctaccgt
    13021 gcgggagctt tccgaggtaa ctgccccctt ggccgccgcg atgtggcgag cagcgttgaa
    13081 acggatgcct attccttttg aggtggtggc acgaacttta cgtcgaattg agatcgacat
    13141 catcgaggac gagactccca gacatagtcg gtttgctttg ttgaaggcct ttaccatcag
    13201 atgtgaaagg gtaccagcca tgacagcaga actgaatgaa cacgaaacgc acccagctta
    13261 cctatgtggg cgaatcatgg ccctacttgg ggccattcag cgggaagcct taggagacgt
    13321 aggtgccggc gtgattcagc ggtattacgc agcagccagt gcaactccag cgctggtcct
    13381 gggacggctg attcgcaccg cccaaatcgc gcacttaccg aagattcagg aagaagggct
    13441 tcgctattgg tttgaaaacc aactcgcgga gctttggaca aagatgagaa gtgtcccacc
    13501 gcgtgtgctc actctggagg aacagacact ctttgcgatg ggttactacc accagatggc
    13561 agcccgattc aagaaaacca gtcaaggagg taacgaaagc gaattggcac agacgaagtg
    13621 acttcttgca gccacgttag ccaatggtgt gaatatgata gcctgacaat aaggtagatg
    13681 ctttgtggag tgtttttaaa gaaccttgca gaaatgaaag gagcttgact atgtcgcaac
    13741 cgattacgag ccgttatgaa tttctctatc tgtttgattg cgaaaacgga aaccctaacg
    13801 gtgatcccga tgcgggtaat gccccgcgga ttgatccgga ggaccttcac ggtttggttt
    13861 ctgacgttgc gatcaaacgt cgtatccgca actacgtgca aatcgccaaa ggcaaccaaa
    13921 tgccctatgc tatcttcgtc gagcaagctt gcaatctcaa caagcatatt gctcgggctc
    13981 acgaggaaac caatggcaaa cttcctgata cgaaaggagc aaaaaaagaa gaagttgaaa
    14041 aagcacgcga gtggatgtgc aaaaattttt acgacgtccg gacattcggc gctgtaatgt
    14101 ccacggggcc aaacgctggt caggttcggg gacctgtcca ggtgacgttc gcccggtctc
    14161 ttgaccctgt gttgcccttg gacatttcga tcacacgcat ggctgtggca gacgacgtga
    14221 gtggtgccaa gtcctggcgc gattacgaaa aatgggaaca agagcaaaaa gaagatacgt
    14281 taaggaccat ggggcgtaag gctcttattc cctatggatt gtatctggga aagggattta
    14341 tcagtgccca tctcgcagag cagaccggat ttagtacgga cgacttgcgt ttgctatttg
    14401 aggcgattct cggcatgttc gagcacgacc gttcggcaag caagggccat atgacggttc
    14461 gccctcctgt ctttgtcctc aaacatgtgg gcacagacac tgaccctcag cagcggaaac
    14521 ggcaagccat gttgggatgt gctccagctc acgtcctttt cgaggaaatc atcgaagtga
    14581 aacgcaagga cggtgttacc gttcctcgtt ccttcgagca ctatcaagtt gtcgttcata
    14641 acgatcgagt tcccaaagga acagagcttc ttcgcctccc ggaggatatg gataagctga
    14701 cctgaagtca tcgcaaaccg gtgcataacg tgaccgttta ctatcgcggc taccgagcgg
    14761 aagacctttt ccgcccggta gccgattcgt ggttctttat atttgctcac accagatttg
    14821 gagccttaat catgttcacc gatgacgagt tggttccaat ttccgctttg gcccaatatg
    14881 tgtattgtcc acgtcgggca gctctcttgt tcctggaacg ccaatgggct gacaatgtgt
    14941 tcacagtcga agggcgacac gtgcaccagc gggcccaccg gaccaatctg cgagagcgac
    15001 ttcccgatgg ctatgcgatc cataaccttc tccttaagtc agaacgattg ggccttgtcg
    15061 gaactgccga cgtcgtcgaa tttcatgaaa aagatcccgc agggccgcgc gtaaccgttg
    15121 ttgaatacaa acggggaaaa cacaaacgtt ccaaacaaac ggagtacgaa gtccaactct
    15181 gcgcacaggt catatgcctg gaagaagtgc ttaacataca aatctcccgc ggtacccttt
    15241 tcttcgctaa gtccaaacac cgttacgacg tcgaatgcca taggacgctg cggacaattg
    15301 tggaagacac aacacaaaag gttcgccagc tttttcaagg gacctgcaca cctgcacccc
    15361 aatacgggcg aaagtgccgt ggttgctccg taatagatat ttgcttcccc aaagttgtta
    15421 gccgaaaaaa cgtccaatac tatatcaagc acatagtcca agagtaggta cgtgataagg
    15481 gactttggac gtaaggatcg caaacgtgaa acaccatctc aacacacttt ttgtaaccac
    15541 acagggcacg tatcttagga aaagtggaga agccgtggcc gtctgtatcg aaaaacagat
    15601 acgactccgc atcccactca tcaacttggg tggaatcgtg tgtttcggtc gcgtgagctg
    15661 ctcgccggcc cttcttgccg cttgtgctcg tgccgggatt tccgtctcct tcttcacaga
    15721 attcgggcgt ttcctcgcgg tctgccaagg ctttacctcc ggtaacgtgc tactacgaag
    15781 gacacagtat cgctgggcag atgatcccga aaaaacggcc atcctagcac gaatgttcgt
    15841 catgggcaaa atcgccaaca cccgttccgt tcttcgccgg gcagcacgcg aacatgaaga
    15901 ccccgcagcc attcgcgata tcagtcagat tgctgatcaa ctgactcaga tactttcaat
    15961 gcttgaacat gcaggtggcg ttgaattggt tagaggcttg gaagggcagg caagtgcggc
    16021 ctatttttct gtctttaatc atttgatcct gacacaaaag gatcatttga agttcacctg
    16081 ccgatcacgt cgtccaccac gggacccggt caacgcgtta ctttccttcc tatactccct
    16141 gttggtggca gacgtccgtg gagcctgcga agcttctggt ttggacgctg gtgtgggatt
    16201 cctccatcgc gacagacctg gacgtcctgg tctcgccctc gacctgatgg aggagttccg
    16261 agcaatcctt gcggaccgcg tggcattaac gctgattaac cgtcagcaga tcaaacccaa
    16321 agacttcatc aatcagcccg gagggacagt cgtccttaca gacagcggcc gtagacgcgt
    16381 ccttgaagaa taccaggcgc gcaaaaatga gacaatcacg catccttttc tcaacgagaa
    16441 aatgacactc ggtctggtgc cccatatcca ggcgagattg ctcgccaggg ttttacgagg
    16501 cgatcttgat acatatcccc catttgtcct caaatgaaag gagaataccg tgttggtcgt
    16561 cgtaacgtat gatgtgtcaa cgaaagacaa ggcgggccag aggcgactca cccgcgtggc
    16621 aaaaaagtgc ctcgactatg gacaacgtgt tcaaaactcg gtgttcgagt gcgtcgtgac
    16681 cccagaacaa tgggttacct tacggaaaga attacttgat ctaatcgacc ctgaggaaga
    16741 cagtcttcgc ttttactttc tcggcagccg aaaccgtcac cgggtggaac acgtcggagc
    16801 caagccgagc ctagatctcg aggagggatt tcttatcggg taacgatatg ggggccgcgt
    16861 accccgagtg cttgcaaaag tgctggaggt tggcggacgt agtaacatgc gatcaaatca
    16921 tcacttgcgg aaatataggc gcgaaataag acatgtggca gacacagaag gcgcaggttc
    16981 gcggaaatga ctgcgtaagt gacgaccatt gtgcgggttg caagggggtg gatcgccctc
    17041 cacgcgaggg cgcggattga aac
//
LOCUS       DAOSUT010000084        16955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2095_length_16955_cov_10.363195, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000084 DAOSUT010000000
VERSION     DAOSUT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16955
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_16955_cov_10.363195"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLD05_09165"
     CDS             complement(<1..941)
                     /locus_tag="PLD05_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP77651.1"
                     /translation="MTRPHENTTSGMTRRSFLAAGSATIVASQLLHSPWVWGAESGPL
                     HVATNFYPWLTFYRRENRDFNADLDASLGEVAAAGLNGFESGLNSPEDVDRLSPFLKK
                     HGLELRSFYVNAVMHEPEAAEKSIAQVLTVAEKAHNVMATRIVVINPSPLSWNREAPN
                     PGKNDAQLETQAKALDQLGAALRERGMQLAYHNHDMELRNAAREFHHMMLATKPENMG
                     LCLDAHWIYRGSGNSQVALFDVVKLYGSRVCELHLRQSKDGIWTEAFGEGDIDYPRLA
                     RELAALGVRPHLVLEQAVEQGSPNTMNAVEAHRRGVE"
     gene            1086..2360
                     /locus_tag="PLD05_09170"
     CDS             1086..2360
                     /locus_tag="PLD05_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP77652.1"
                     /translation="MRSLPSQLAVIGFVIGFLTMQTSLLAAEVAWPEPVVPAGFGVNI
                     HFTDPRPGEMSMLAASGLKWIRMDFVWSATEREPGKYDFRAYERLLKALDEYGIKALF
                     ILDYTNRHYDQGLSPYTDEGRQAFARWAAAAAKHFQGRGILWEMYNEPNIHFWKPKPN
                     VEDYIKLAIEVGKAIRREAPGEIYIGPATSQIDMKFLEACFQAGLLEYWDGVSVHPYR
                     QSPPETVLAEYQKLAELIAKYAPAGKKIPILSGEWGYSAVWRNYDETRQGKYLPRQWL
                     TNLAAGIPVCIWYDWHDDGPDPKEPEHHFGMVAYPYHEGRNPVYDPKPAYLAAQTLCT
                     LLAGYRFVERLPVKAEKAELCHALRFEKGGKSAVVIWYQQDGQTTLVQVALPPGLYRV
                     IDYLGKDLGTKNVVAASTELEITDAPLYLVQE"
     gene            2564..3961
                     /locus_tag="PLD05_09175"
     CDS             2564..3961
                     /locus_tag="PLD05_09175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP77653.1"
                     /translation="MKLQFLGANRQVTGSQYYLQAGKTRLLIDCGMFQERPYLDRNWD
                     PLPVPPREIDALLLTHAHLDHSGLIPKLVREGFRGKILATSASVDVAEIVLRDSAHIQ
                     VEDAAYKRKRHRKEGRTGKHPEIPLYDVNDVEEALPYFRAVPYYQPIELDGVTIRFLD
                     AGHILGSAFLVVDAKENGRERRIVFSGDLGSPDRPILRDPDMIHEADYLVIESTYGDR
                     DHNHGPSVEDQLVKVVRETSARGGHVVIPVFAVERAQELLFYFSRLYRSGRLPPLPIF
                     LDSPMAVRITEVFHRHRECFDQETQNLIDNDQSPFNFPNLRLVTTVEESKQINTYNVP
                     SVIMATSGMCTAGRIKFHLRNNIVRPESTILFVGYQSRGTLGRQILDKNPYVRIHGKE
                     WPTRARIEQIQGLSGHADRKGLLSWVSQVKSPVKSVFVTHGEENASLSFAEMIRTQLN
                     WRTSVPQYMDEVELD"
     gene            complement(4032..4838)
                     /locus_tag="PLD05_09180"
     CDS             complement(4032..4838)
                     /locus_tag="PLD05_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOP77654.1"
                     /translation="MSDSRFEATLDSQNLMCGELLKTSDALDVPPEKRPRHIAAIMDG
                     NGRWAQRRGLPRIEGHRHGVAAVRRTVEECVRLGIEQLTLYCLSSENWKRPKFELDFL
                     LRLLEQYIVEERGTFTENNVRVQVIGRREGIPPEVQRELDKTLQLTANNTGLCLCLAV
                     NYGGRVEILDAVRKIAEKVVAGQLSINDIDEATFSDHLYTAGMPDPDLLIRTAGEQRV
                     SNFLLWQISYAELWITDVCWPDFNEELLHQAIRDYSRRVRRFGGLVRSPS"
     gene            complement(4975..6258)
                     /locus_tag="PLD05_09185"
     CDS             complement(4975..6258)
                     /locus_tag="PLD05_09185"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOP77655.1"
                     /translation="MSATAVIGLQWGDEAKGKIVDLLTEDHEIVVRYQGGANAGHTVV
                     RQGQTFKLSLIPSGIFQPNVTCVIASGVVVNLKTLLDEIALLRSRGVSISNNLLLSDR
                     AHVIFPWHLEEDRILNECPSGEAIGTTLRGIGPCYRDKVGRSHAIRVGDLFESGFKER
                     IEAIVQAKARFLVPLGVSPDSPSLDAAAIFREYSGYAEAIRPFVCDTTEFLLTAAERE
                     RRILLEGAQGALLDVDHGTFPYVTSSNSSGAGISAGSGIPAKYIKKVLGVVKAYTTRV
                     GGGPFPTEQDNPIGQTIRDRGNEYGTVTRRPRRCGWFDAVAVRYTARLSGVDAIAVML
                     LDVLSTLPELKICVAYELDGERITHFPSHVEQLRRVKPVYETLPGWSEEIQHVRRLQD
                     LPKAARAYLDRLAELLRCPIEMVSVGPDREQTIIC"
     gene            6646..7554
                     /gene="ispE"
                     /locus_tag="PLD05_09190"
     CDS             6646..7554
                     /gene="ispE"
                     /locus_tag="PLD05_09190"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOP77656.1"
                     /translation="MDVQRCGTTWVVHAPAKVNLFFEVHGRREDGYHLVETLVCPISL
                     YDTLAFEENPDDDVVLSVQFITGGASLPECGFDTVPDGPSNLVYRAAELVKKETGTRK
                     GAKIRLWKRIPTGAGLGGGSSDAAATLLGLCRLWGLTPGMAELRAWAARLGSDVPLFL
                     EGGPCICRGRGEIVNRVNGFTRLDMVIVKPRVSLSTASVYAACRVPEVPQRIEAILAA
                     WSSGDRKRLGEFLFNRLEEAAKRIAPSVDEVGARLRQTDCLGASMTGSGSCCFALYWH
                     RKHAIAVARRFMAEKVGAVFVVHTVA"
     gene            7582..8118
                     /locus_tag="PLD05_09195"
     CDS             7582..8118
                     /locus_tag="PLD05_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOP77657.1"
                     /translation="MQITEVRIKLMDEPGERLKAFCSITFDDCFVVRDLKIIEGANGP
                     FVAMPSRKLTARCPNCRTKNHLRAQYCNQCGIRLKEPPIARDEEGRAKLYADIAHPIN
                     SQAREMIQSRVIKAYEEEKARSKMPGYVPPVYDEYDEEQTAQAAERPYDVVRRKAHSG
                     KEEIKQPHQSREGVPQKD"
     gene            complement(8218..8292)
                     /locus_tag="PLD05_09200"
     tRNA            complement(8218..8292)
                     /locus_tag="PLD05_09200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8255..8257),aa:Pro,seq:cgg)
     regulatory      8580..8651
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            8780..9223
                     /gene="crcB"
                     /locus_tag="PLD05_09205"
     CDS             8780..9223
                     /gene="crcB"
                     /locus_tag="PLD05_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOP77658.1"
                     /translation="MERFRLLGTLALVTLLSSDVVLIGLGGGLGSVARYLVGKLIHEG
                     SGENVFPIGTLVVNVLGCFFLGMLIGVFLEQGSHRVTGWLNFLGTGFCGGFTTFSALQ
                     WETVRLIHDGRWVVAAVNVGLSIFAGLVGIVVGYLIGRWVSFYVD"
     gene            complement(9476..10465)
                     /locus_tag="PLD05_09210"
     CDS             complement(9476..10465)
                     /locus_tag="PLD05_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HOP77659.1"
                     /translation="MVPDSQERAPWITYRPELKVLDCTIRDGGLVNDHHFSDEFVKGV
                     YQTCVEAGIDYMEIGYKASKKVFQRDRFGAWKFCDEDDVRRIVGDNPTSLKLAVMMDA
                     GKSDWKTDLLPKDRSVVDMVRVAFYVHQTVEACEMIKAAHEKGYETSANLMAVSQATE
                     VEIEQCLGLISQTPAEVVVVVDSFGALYTEQIERLVKIYLSYVKDKGKTVGIHAHNNQ
                     QLAFANTIEAIIHGANRVDASMGGLGRGAGNCPMELLIGFLRNPKFRIRPVLQFLQDY
                     IEPLRNEVEWGPYVQYNITGQLNLHPRSAIEARKNPEDRNKYVDFFDRVSTEA"
     gene            10775..10993
                     /locus_tag="PLD05_09215"
     CDS             10775..10993
                     /locus_tag="PLD05_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOP77660.1"
                     /translation="MAEVTITVRPRGPYLVQGNVRIVDADGNEYPVDPSKPVALCRCG
                     ASQKRPFCDGAHKSCGFEGDDRATPPGA"
     gene            11783..12559
                     /locus_tag="PLD05_09220"
     CDS             11783..12559
                     /locus_tag="PLD05_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOP77661.1"
                     /translation="MSSLFGLITNKPADLALSLRGPSADHPNVKPAAAWGIGWYEGSR
                     PRVAKQIHLAGGSLCDKSEFTPGASRLFLVHFEEKVSGGEKETDLCPFHYRHWLFSHT
                     GELDREAFVHLLEPAHVQSLNGESGSEVFFHWIMQNIKKTRDLVPSLLDSLRDVSENK
                     LSFLLSNGRWLFAYARGQKLYYHERRFPTSAGTFVIRVPNVDAVISSTILANESAILV
                     STDKLGDEAWEPVPDGHLLAVTPRLDVILIDLAENSPSPT"
     gene            complement(12626..13462)
                     /locus_tag="PLD05_09225"
     CDS             complement(12626..13462)
                     /locus_tag="PLD05_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOP77662.1"
                     /translation="MPQPLRYLQIFATFARNSLIRDMTFRANFLIEFISSLSWVFMNL
                     GFYILIFHYTPSLGRDTGWGKYEFFVFTGTALMINSLAGMLFMTNADNFGELIRRGGL
                     DFVLVKPVDTQFLVSLQRVDWSSSGNFLVGLGLAVYSLRCLGVWPSPVTIVLYVFYLL
                     CGVVILYSLMFLLAATAVWLGRNETLFDFWFYIVSFGRYPMEIYEGRFGRPFRWFFTF
                     IIPVLVAINVPSRFLVQSIRPQSLEEWLLPLYTIAATVIVFAVCRAVFQLALHSYRSA
                     SS"
     gene            complement(13466..14338)
                     /locus_tag="PLD05_09230"
     CDS             complement(13466..14338)
                     /locus_tag="PLD05_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOP77663.1"
                     /translation="MTYPSPAIIDTISAPLYRWRLWITILRISIQERIIYRGDFMLGT
                     LMRFLPIITQIFLWGAVFSALPTGNGSEGRIVGYSYHDMVAYYLLTMLSRAFSSMPGL
                     ASGITREIREGTIKRFIVQPIGLLEYLFFYRLAHKLVYYSVAIVPFAVVFYLCRGFFQ
                     ETPHGLVWATYIASLVLAFLLGFFLEATLGLLGFWFLEVSSFLFIYMLLSFFFSGHMF
                     PIDMLPEPMATVVKLIPLQYLAYFPAAVFLGKVQGAALVTGLLVQAGWVIVFAFLAQV
                     ALRRGLRHYSGFGG"
     gene            complement(14385..15431)
                     /locus_tag="PLD05_09235"
     CDS             complement(14385..15431)
                     /locus_tag="PLD05_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOP77664.1"
                     /translation="MAAIEIRGLSKTYRVTRKKEGLVATLRALFRPEYEYVHAVRSID
                     LTIESGEFVAFLGPNGAGKTTTLKLLSGIITPTSGEARVLGHIPWRRENAYRRRFALV
                     MGQKNQLWWDLPAAESFHLHQQIYGIPPDQFTRVRDDLTEMLGVRHLIHRPVRELSLG
                     ERMKMELVAALLHSPQVLFLDEPTIGLDVVAQYTIQQFLREYQARTGTTVILTSHYMK
                     DVVVLCRRVVVIFDGQIIYDGSLTNLVDHLGDYKIITIRFSDGQVPTDLSRFGKIILR
                     EPPRVRLRVARNEATEVIRAVLARYIAEDVSVEDPPLEELIAALFASARNSRLSQERA
                     NSVVPEDTTHSTNH"
     gene            15679..>16955
                     /locus_tag="PLD05_09240"
     CDS             15679..>16955
                     /locus_tag="PLD05_09240"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOP77665.1"
                     /translation="MPPHGQRETWTTRIGVILAVTGSAVGLGNFLRFPGLAAQYGGGA
                     FMVAYLCAFLFLGLPIAFAEWAMGRYGGMHGHNSSPGIYRVIWKSRAAPYFGILAMIV
                     PVGIYMYYVYIESWCLAYACRYLAGAMNFGSDAARYREFFEHFVGIHNHGESLSTPGE
                     GLLGSAVFFLVVCFVFNFILIYRGLSKGIEWFCRWAMPALVICAIIILIRVLTLGTPD
                     PSKPEQNVLSGLGYMWNPVKPGMSFWQALADPEMWMKAASQIFFSLSVGFGIIITYAS
                     YVKKDDDIALSSLTAAAGNEFCEVVLGGLMVVPAAFVFLGPQIGQQLGNPFALGFMAL
                     PSVFNQMPVGWLFGCLFFFLLFLAAITSSLSMLQPAVALLEEGLGLNRRASVAVLSFI
                     TAIGSAMVVYFSKDFVALDTLDFWVATFAIYLLA"
BASE COUNT         3847 a         4623 c         4685 g         3800 t
ORIGIN      
        1 tactcgacac cgcgccggtg agcctcgacc gcgttcatcg tattgggcga tccttgttcc
       61 acagcctgtt cgagaacgag atggggacga accccgagcg ctgccagttc ccgcgcgagc
      121 cgcggatagt cgatatcgcc ctcgccaaag gcttccgtcc aaatgccgtc tttcgattgt
      181 cgcagatgaa gttcgcagac acgcgagcca taaagtttca cgacatcaaa cagggcaact
      241 tgcgaattcc ccgacccgcg ataaatccaa tgtgcatcca ggcacaggcc catattctcc
      301 ggcttcgtgg cgagcatcat gtgatgaaat tcccgtgccg cattccgaag ttccatatcg
      361 tgattgtgat aggccagttg catcccccgc tcacgcaagg ctgcgccgag ttgatcaagg
      421 gcttttgctt gagtctccaa ttgcgcatcg tttttgccag gatttggcgc ttcccgattc
      481 caggacagtg gagacggatt gatgacgaca atccgcgtcg ccataacatt gtgggccttt
      541 tcagccacgg taagcacctg ggcaattgac ttctcagcgg cctctggttc gtgcattacg
      601 gcattgacat aaaatgatcg cagctccaat ccgtgctttt tcaggaaagg tgagagacgg
      661 tccacgtcct ccggagaatt gaggccagat tcgaaaccat tcaatcctgc agcggcaact
      721 tcacccaagc tggcatccag atcggcgttg aaatcgcgat tttcgcggcg ataaaacgtt
      781 aaccagggat aaaaattcgt cgccacgtgt aaaggacctg attctgcccc ccacacccat
      841 ggcgaatgga ggagttgact tgcgacaatg gtggctgaac cagcggccaa gaaactgcga
      901 cgtgtcatcc ccgatgttgt gttttcgtgc ggccttgtca taatgagatt ctccgtttta
      961 ggtcgttggt gacaaatctg tctgtcaatt ccgaaaaaga cgcccctaat ataccgcccg
     1021 ccgaaaccga ctcgcaagat acggcctcca cagaagcgtt aagacataag aaaggagtca
     1081 tcgccatgag gagcttacct tctcaattag ctgtgatcgg ctttgtcatc gggtttttga
     1141 ccatgcagac ctcattgttg gcggctgagg ttgcctggcc agagccggtc gttcccgctg
     1201 gcttcggcgt caacatccac tttacggatc cccgaccggg cgagatgagc atgcttgcgg
     1261 cctccggcct caagtggatt cgtatggact ttgtctggtc ggcaacagaa cgggagcccg
     1321 ggaaatatga cttccgggct tacgagcggc tcttaaaggc cctggacgaa tatgggatca
     1381 aagccctttt catcctcgac tataccaatc ggcattatga ccaagggtta tcaccctaca
     1441 cagacgaagg gcggcaggcc ttcgcccgat gggcagcggc ggctgccaaa cacttccaag
     1501 gacgtggcat cctttgggaa atgtacaatg agcccaatat ccatttctgg aagccaaagc
     1561 ccaacgtgga agattacatc aagctggcga ttgaggtggg aaaggccatt cggcgggagg
     1621 cccctggtga aatttacatt ggaccggcca cctcgcaaat cgatatgaag ttcctggagg
     1681 cctgttttca ggcgggactg ctggagtatt gggatggcgt ctcggtccac ccgtatcgac
     1741 agtctccccc ggaaacggtt ttggcagagt atcaaaaact ggccgagttg atcgccaagt
     1801 acgccccggc gggtaaaaag ataccgatcc tctctgggga atggggctat tcggcggtgt
     1861 ggcggaacta cgacgaaacc cgccaaggga agtatcttcc tcgacaatgg ttgactaacc
     1921 tggcggccgg tattcctgtt tgcatttggt acgattggca tgacgacggg ccggacccca
     1981 aggagcctga gcatcatttt ggcatggtgg cttatcccta tcatgaggga cgaaatccgg
     2041 tttatgatcc gaaacctgcg tacttggctg cccagacgct atgcacgtta ttggcaggat
     2101 atcggtttgt cgaacgtctg ccggtaaagg ccgagaaggc cgaattgtgt catgccctga
     2161 ggttcgaaaa gggtgggaag tccgccgtgg taatctggta tcaacaagat ggccagacga
     2221 ctttggtcca ggtcgctctg ccaccgggcc tttatcgcgt catagattac cttggaaaag
     2281 acctgggcac caagaacgtg gttgcggcct cgaccgagct tgagatcacc gacgcgcccc
     2341 tctatcttgt ccaggaataa tcgcggacag actggtcttg atgtttctgt caaaagaaac
     2401 aaacattttt cgctatgcca acgtcctata agtagggccg cttgaaaaaa gggtatcgat
     2461 tgtgcctttc ctacaagttg gtcttacatt gcggtcttcc cgatccgatg ggttatgggg
     2521 gtaacttgag cgtcgcacat ttggcattcg acgaggatga gcagtgaaac ttcagtttct
     2581 gggtgccaat cggcaggtta cgggttccca atactatttg caggcgggta aaacccggct
     2641 tcttattgat tgcggtatgt tccaggagcg tccgtacctg gaccgtaatt gggacccttt
     2701 acccgtgcca ccgcgggaaa tcgacgccct ccttttgacc catgcccatc ttgatcacag
     2761 tggcttgatc cccaagctgg ttcgagaggg tttccgcggg aagatccttg ccacatctgc
     2821 ctccgtcgat gtggcagaaa ttgtccttcg ggattcggcc cacattcagg tggaagacgc
     2881 cgcttacaag cggaagcggc accgcaagga ggggcggacg ggcaagcatc cggaaatccc
     2941 cctctacgat gtgaatgatg ttgaggaagc cttgccctat tttcgtgccg tcccatacta
     3001 tcagcctatc gagcttgatg gggtaaccat ccgctttctt gatgccggtc acattttggg
     3061 ctcggcgttt ctggttgtgg atgcaaaaga gaatggtcgg gagcgacgga ttgttttttc
     3121 gggcgacctg ggcagcccgg accgtcccat ccttcgtgat cctgacatga tccacgaggc
     3181 cgactacctc gtcatcgaat cgacgtacgg tgaccgtgac cacaatcatg gtccaagtgt
     3241 ggaggatcag ctcgtcaaag tcgttcggga aacatctgcc cggggcggtc acgtggttat
     3301 tcccgttttt gcggttgagc gggctcaaga gttgctcttc tatttcagcc gcctatatcg
     3361 gtcgggacgc cttccaccct tgcccatctt cctggacagt ccgatggccg tgcgaatcac
     3421 tgaggtcttc catcgtcacc gtgagtgttt cgaccaagag acgcaaaacc tcatcgacaa
     3481 tgatcaatcg cccttcaatt ttcctaactt gcgactggtg acgaccgttg aagaatccaa
     3541 acagatcaat acttacaatg tcccgtccgt gatcatggca acttccggga tgtgcactgc
     3601 tgggcggatc aagttccatt tgagaaacaa tattgtgcga ccggaatcga caattctttt
     3661 tgtgggttat caatctcgcg ggacgttggg acgccaaatc ctcgacaaga atccgtatgt
     3721 tcgtattcac gggaaagaat ggcccacccg agcgaggatc gaacaaatcc agggcctatc
     3781 gggacatgcg gatcgtaaag ggcttctcag ttgggtttcc caagtcaaat ccccggtgaa
     3841 gtcggtgttt gtgacccacg gcgaagagaa cgcctcctta agcttcgcag aaatgatccg
     3901 cacccagctt aactggcgga cgagcgttcc tcagtacatg gatgaagtgg aattagattg
     3961 acccaccggg ctgaagcgca cgacatttcc gccgaacggc tgtgagcttt gcgtgataag
     4021 aaccctggca atcacgaagg cgatcggacc aggccaccaa atcgccgaac ccgccgggaa
     4081 taatcccgga tggcttgatg tagcaattcc tcgttgaagt ctggccagca gacatccgtg
     4141 atccacaact cggcgtagct aatttgccag agcaaaaagt tactcactcg ctgctcgcca
     4201 gccgtgcgaa taagcaaatc ggggtccggc atgcccgccg tgtagaggtg gtcggagaac
     4261 gtcgcctcgt caatgtcgtt aatggataat tgccccgcaa ccactttctc tgcgatcttg
     4321 cgaacggcat ccaggatttc cacccttccg ccgtaattga cggccaaaca caggcacaat
     4381 cccgtgttgt tcgcggtcaa ttgcaaggtc ttatcaagtt cgcgctgcac ttcgggcggg
     4441 ataccttctc tccgtccgat cacttgcacc cgcacgttgt tttctgtgaa cgtcccccgc
     4501 tcctcaacga tgtactgctc cagtaatcgc aaaaggaaat ccagctcgaa cttgggccgc
     4561 ttccaattct cgctggagag gcaatacaaa gtgagctgtt caattcccaa tcggacgcac
     4621 tcttcgaccg tccggcgaac agccgccaca ccgtgacggt gtccctcaat gcgaggcaac
     4681 ccccgccgct gcgcccagcg accgttgccg tccataatgg cagcgatatg ccggggccgc
     4741 ttctccggtg ggacatctaa agcgtcggac gtttttagca gctctccaca catcaggttt
     4801 tgggaatcaa gcgtcgcctc aaatcgggaa tctgacatct tgccctaaca tccagtttag
     4861 caaaaacatt cgcccggcga ccatgtgggg tttgccacaa gtcctgtcat ttcccttgaa
     4921 aatagccgtc gggtgtggag gcttgaagca cacactctga aggacgaagc ccgtttagca
     4981 aatgatcgtt tgctcgcggt cggggcccac agaaaccatc tcaatgggac accgtagtaa
     5041 ctctgccagg cgatcgagat atgcccgcgc tgccttgggc aagtcctgca agcggcggac
     5101 gtgctggatc tcctcggacc atccggggag tgtctcatac acaggcttca cgcgtcgcag
     5161 ttgctcgaca tggctgggga aatgggtaat ccgctccccg tccaattcgt aagccacgca
     5221 aatctttagc tcgggcaacg tgctcagaac atccaggagc atcacggcaa tagcgtcaac
     5281 gccactcagt cgagccgtgt aacgcacggc aacggcatcg aaccagccgc agcgcctcgg
     5341 gcggcgggtg accgttccgt attcgtttcc ccgatcccgg atggtctgac cgatagggtt
     5401 gtcctgctct gtcgggaaag gcccaccgcc gacacgtgtt gtatatgctt tgactacccc
     5461 caaaaccttt tttatatact tggccggaat ccccgagcct gccgaaatcc ccgcaccgga
     5521 gctattgctg ctcgtcacat agggaaaggt tccatggtca acatcaagga gggctccttg
     5581 tgccccctct aggaggatcc gccgctcacg ttccgcggca gtcaacaaaa actccgtcgt
     5641 gtcgcacaca aaaggtcgga tggcctcggc gtaaccggag tactcacgga agatggcggc
     5701 cgcatcgagg gacgggctat ctggcgaaac ccccaggggg accagaaacc gggccttggc
     5761 ctgcacgatg gcctcaatcc gctctttgaa accagactcg aacaggtccc ctaccctaat
     5821 ggcatgcgac cgtcccacct tgtctcggta acatggccca atgcctcgaa gcgtcgtgcc
     5881 aatagcctca ccgcttgggc actcgttgag gatacgatcc tcctccaagt gccatgggaa
     5941 gatgacatgg gcgcgatcac tcaaaagaag attgttcgag atggagaccc ctcttgagcg
     6001 gagcagggca atctcatcga gaagcgtctt caaattgacg acaacgccgg acgcgatgac
     6061 acacgtcacg ttgggttgaa agattccgct agggatcagg gacagcttaa aagtctgacc
     6121 ttgtctcacg acggtatgac cggcgttggc cccgccctga taccgaacca caatttcgtg
     6181 gtcttcggta aggagatcga cgatcttccc ttttgcttcg tcgccccatt gcagaccaat
     6241 gacagcggtg gcgctcacga gattcacctt tctcttgtta gtcaacacga tcacgaaagt
     6301 ccgtcgtcca acctatgccg ttcccaaagg ccgaaattcc gaaaacaagc gcgagcggaa
     6361 gcccgcgcgt ttgtggtttg cgcggctaga accgctggtc ccctccgtgc cacggccagt
     6421 ttcgtctaga acagctccag agttgcagtc taatcgccat cagttcgaac cgtcaaggaa
     6481 ccgcacgctc ggaacttcgg accggggaga aggctcaatt cgtccacaca cgtgttcatc
     6541 acccgcagcg atattgacag ggacggcaaa accattaaaa tcccgattgc tggggggatc
     6601 gcgttggcaa gcggatggga aatctaaagc cggggccgac acggaatgga tgtccagcgt
     6661 tgcggcacaa cttgggtcgt tcatgcccct gcaaaagtga accttttctt cgaggtgcac
     6721 ggccgccgcg aagacggata ccatctcgtc gagacgctgg tttgtcctat cagcctctac
     6781 gataccctcg cttttgagga aaatccggat gacgacgtgg tcctcagtgt acaatttatc
     6841 acgggagggg cgtcgctccc ggagtgtggt tttgacacgg ttcccgacgg cccgagcaac
     6901 ttggtttatc gggccgccga actggtaaag aaagaaaccg gcacccgcaa aggtgcgaag
     6961 atccgtttgt ggaagcggat tccgacaggc gcagggttgg ggggagggtc ttccgacgcg
     7021 gcagccacgt tgttgggttt gtgccgtctc tggggattaa ctcccgggat ggcggaacta
     7081 cgagcgtggg ctgcgcgcct gggaagtgat gtacccctgt tcctggaagg aggcccctgc
     7141 atctgtcgcg gacgcggcga gatcgtaaac cgcgtgaatg ggtttacccg gcttgacatg
     7201 gtgatcgtca aacctagggt aagcctatcc acggccagtg tttatgcggc gtgtcgtgtc
     7261 cccgaagtac cgcagcgtat tgaggcgatc cttgccgcgt ggtcatcggg ggaccggaaa
     7321 cgactcggcg aattcctgtt taaccgtttg gaggaagccg caaagagaat cgcaccgagt
     7381 gttgatgagg tgggtgcccg tttgcggcaa accgattgtc tgggagcgag tatgacggga
     7441 agtgggtcgt gttgcttcgc gctgtattgg catcggaaac acgccatcgc ggtggcacgg
     7501 agatttatgg ctgagaaggt gggagccgtc tttgtcgtgc acacggtggc ctagcgtgcc
     7561 ccttgggaaa ggaggtcggc cgtgcaaatc actgaagtcc gcatcaagct catggacgag
     7621 ccaggagaac ggctgaaggc cttttgctcg atcacgtttg atgattgttt cgtcgtccgg
     7681 gacttgaaaa ttatcgaagg ggcgaacggt ccttttgtgg ccatgcctag ccgcaaactg
     7741 accgcgcgct gtcccaactg ccgcacgaag aatcacttgc gggcccaata ctgcaatcag
     7801 tgcggcatcc gcttgaagga gccgccgatc gcccgggacg aagaagggag ggcaaaactt
     7861 tacgctgata ttgcccatcc catcaattcc caggcccgcg aaatgatcca atcccgggtc
     7921 attaaggcct atgaagagga aaaggcccgc tcgaaaatgc cgggttacgt cccgccggtc
     7981 tacgatgagt atgacgaaga gcagacagct caggcggccg aacgtccata tgacgttgta
     8041 aggcggaagg cccacagcgg caaagaggaa attaaacagc cgcatcagtc gcgggaagga
     8101 gtaccacaaa aggactaggt aaatccccct tccggcggct agttacagtc gcgtgtcggc
     8161 cgatgttcag cgacgaaaag cagaacgtag gatgaggcac atctcccgct catctaatcg
     8221 gggcgactgg atttgaacca gcgacctctt ggtcccgaac caagcgctct agccaggctg
     8281 agctacgccc cgggcgttaa ctcattagaa aaggccaacc gggcggcaaa aaacgccctc
     8341 gctgacccat atccgtcgtc cttaaagtgt aatcggttga tttggacgcc gtcaatcggg
     8401 agccattaag cctttcaagc aaaaggagag ccagccgaaa atattacgtc tcagggattg
     8461 ctaagccgat gtaacggaat aatcgtcaaa ggttaccagg cttggcgacc gccacgcccg
     8521 cggaagtttt ttgggccagc ccctcttccc tggcggacgg gataagctat atttaaagaa
     8581 agtataatgg cgatggggtt cgccactaat tgtagcttcg catgctagct actgatgacc
     8641 cctacctggt tttcggtagg ggttttcttt tggccttgcc ctaccgaagt ggttaactcg
     8701 gcggggatct cacggccggc caccgagaat cctcgtgcag cggttggtct cacactgtaa
     8761 agggatctgg gttcgcaaca tggagcgttt tcgtttgctt ggcacccttg ctttggtcac
     8821 gttgctatct tccgacgtcg tcctgatcgg tctgggaggt ggacttggtt cggtcgcccg
     8881 ctatctcgtg ggaaagctaa tccacgaggg aagtggagaa aatgtctttc cgataggcac
     8941 gctggttgtc aacgttttgg gatgcttttt cctcgggatg cttattgggg tttttctcga
     9001 acaggggagt caccgggtga ccggatggct gaacttcctg ggcaccggat tctgcggagg
     9061 atttacaacg ttttcggctc tccaatggga aaccgtccgg cttatccacg acggccgctg
     9121 ggttgtggcc gccgtcaacg ttggcctcag tatttttgcc ggcttggtcg gtattgtcgt
     9181 cggttacttg atcgggcggt gggtatcttt ttatgtggac tagccgagat tgcgagcgat
     9241 tcttgacacg atcgtcggtc gaatttcaag acacctaatc gcaaactcag ccagcaagaa
     9301 atttccgcac ctcgttccaa ttcgctggcc catagccgat cgccattgcg aggaggaacc
     9361 cggctacgaa attgacgatt cctgtgccga tgataaaaag aatgacgtac aatggtccgt
     9421 ccctacttcc ttcttcagtc agaaagctat caccgctgac cggacaaata ccgtcttacg
     9481 cctcagtgct tacccgatcg aagaaatcga cgtacttgtt tcggtcttcc gggttttttc
     9541 gggcttcaat cgctgatcgc ggatgcaggt taagttggcc cgtgatgttg tactggacat
     9601 aaggccccca ttccacctcg ttgcgcagcg gctcaatgta atcctgcaag aattggagca
     9661 cggggcgaat ccggaactta ggattgcgga ggaagccgat caggagctcc atgggacagt
     9721 tgcccgctcc ccgccccaaa ccacccatgg atgcatcgac gcggttcgct ccatgaatga
     9781 tcgcctcgat ggtgttggca aaagccagtt gctgattgtt atgggcgtgg attcccacgg
     9841 ttttgccttt gtccttgaca taactcaaat aaatcttaac aaggcgctca atttgctcgg
     9901 tataaagtgc gccgaaactg tcgactacga cgacgacttc agcaggcgtc tgcgatatga
     9961 gcccgaggca ttgctcgatt tcgacctcag tggcttgtga gaccgccatc aaattagccg
    10021 acgtctcata acctttttcg tgggcagcct tgatcatctc gcaagcttcg acggtttggt
    10081 ggacatagaa agccacccgg accatgtcca caacgcttcg atcctttggc agcagatccg
    10141 ttttccagtc cgatttaccg gcatccatca tgacggcgag ttttaatgac gtgggattgt
    10201 caccgacaat tcgccggaca tcatcctcat cgcagaattt ccacgccccg aaacggtcgc
    10261 gctggaagac ttttttcgat gctttgtaac caatctccat gtagtcgatg cccgcttcca
    10321 cgcacgtttg atacacccct ttcacaaatt cgtccgagaa atgatggtcg ttgacaagtc
    10381 ccccatcgcg aatcgtgcaa tctagcacct ttaactccgg acgataggta atccagggcg
    10441 cgcgttcctg agagtcgggt accatatctt tcggttcctt tctgtcgtca atcgctcact
    10501 gtcacaaacg gagtcatctt gtgggaaagg acacttgcca cccggctttt caccgaatcc
    10561 tagacccgcc aaggaaacta tatcttacca aaactcatgc gcctggggtg tacaaggtga
    10621 cgtttttggg tgttcgaact gcaaaagtag gcgtgtcttc gtcgtgggac cttgaccgcc
    10681 ctcatagact tgaggatcgg ctgagggcca cacccttcgg ctggattatt cggaatgacc
    10741 catacaaagc gtcaattcgg gaggtaccct gtcaatggcg gaagtgacga tcacggttcg
    10801 gccccgcggc ccttatcttg tgcaaggaaa tgttcgtatc gtggatgccg acggaaacga
    10861 atatcctgtt gatccttcca agcccgttgc cctgtgtcgc tgtggggcct cgcagaaaag
    10921 accgttttgc gacggtgccc ataagtcgtg cggattcgag ggtgacgatc gggcgacacc
    10981 tcctggcgcg taacacccga ggcgagtctt cccgctcgga cggcatgttc agcaaaacgc
    11041 tcagcgttcg cgtgcattct tcggggcgag cgcggttcgc atgttttcgg taaccgtcca
    11101 actccaggtt tctggaataa tgtcccagat cacaaaagtg accaaaaggc cggaaatgat
    11161 cgggcttgta ggagctatcg ggccggttgg accttcggag tcatctcgag aaatggcgaa
    11221 aaaaagggat ttaaccaaaa gcccatccca aagttccctc tccctctggg agaggttcgc
    11281 ggtgagggcg agcgatcgtc gcagtcgatc gagaaaaccg gcagcgattc gagtcaccct
    11341 cacccccggc ccctctttcg ccgaacggga gaggggaggt tgtgggatag gttctaagga
    11401 cgcccgccaa cgtcggtggt aaaaagagtt cgtctctccc ccaccataga gctatccgtg
    11461 gggtgtttga cgacgttggt tgacggccaa cccatgcagt ttgcgcgttt taggggaata
    11521 atacggtcgt tccgcaagta aattgctttc agcaaggcgg gcgccttagt aaactggaag
    11581 aaattgccgt gatgctccaa ggaggtcgca tcacagtcgg gccagcctgt ggaccggtgc
    11641 acgggcaaaa ccggcggtcc ataccttggg gtaagcgtct gtttagcatc gaggtttttc
    11701 gatgtccagg gggctttcga acgtgaagga tggagggcag aaaaagggca acgtcgcctg
    11761 atttttggag gaaaacgcat cgatgagttc gctgtttgga ttgatcacca ataaaccggc
    11821 agatttggcg ctttccctgc ggggcccgag cgcggatcat cccaacgtca agccggcggc
    11881 agcctggggc atcggttggt atgaaggatc gcgaccgcgg gtagctaagc aaattcattt
    11941 ggcaggtggt tccctctgcg acaagagtga gtttacccca ggggcatcgc gattgttctt
    12001 ggtccatttt gaggaaaagg tctccggcgg ggaaaaggaa accgatctct gtccgtttca
    12061 ttatcgccat tggctattta gccatacggg cgagctcgat cgcgaagcat ttgtccacct
    12121 cctggaaccc gcgcatgtcc agtcgctcaa tggtgagagc gggtctgagg tgttttttca
    12181 ctggattatg cagaacatca agaagacgcg ggacctggtt cccagccttc ttgattcgtt
    12241 gcgtgacgtg tcagaaaaca agttgagttt tctcctcagc aacgggcgat ggctctttgc
    12301 atatgctcgt gggcaaaagc tttattacca cgaacgtcgg tttcccacat cggccgggac
    12361 gttcgtcatc cgtgtgccca atgtggatgc ggtgatctcc agcaccatcc tggccaacga
    12421 gagcgccatt ctggtttcga cagacaaact cggtgacgaa gcgtgggaac ccgttcccga
    12481 tggtcacctc ttagccgtga cacctcgcct cgacgtcatc cttatcgacc tggcagagaa
    12541 ttcaccttcc cccacctgaa gagaaagccc ctctttcagt cgccgctgac tcgtgcgacc
    12601 agcatgttcg cacggtggcg gtattttaac tactcgcact gcggtaacta tggagggcaa
    12661 gttgaaaaac ggctcgacaa accgcgaaga caatgaccgt tgcagcgatg gtgtaaaggg
    12721 gtaggagcca ttcttccaag gactgcggtc gaatcgattg gaccaaaaag cgcgagggga
    12781 cgttgatggc aaccaagacc ggaatgatga aggtgaaaaa ccatcgaaaa ggccgcccga
    12841 agcgtccctc gtaaatctcc atgggatacc tgccaaaact gacgatgtag aaccaaaagt
    12901 caaatagtgt ctcattacgg cctaaccata cggccgtcgc tgccaaaagg aacatcaaac
    12961 tgtaaaggat gacgacaccg caaagcaggt aaaagacata aagaacgatt gtcaccggcg
    13021 agggccacac cccgaggcat cgcaaggaat agacggctaa tcctagcccc accaaaaagt
    13081 tgccgctcga agaccaatcc acgcgttgca acgacacgag gaactgcgtg tcaaccggtt
    13141 tgacgaggac gaagtccagg ccgccccgtc ggatgagttc tccgaaatta tccgcattgg
    13201 tcatgaataa catgccggca aggctgttaa tcatcagtgc ggtgccagtg aagacgaaaa
    13261 actcgtactt accccagccg gtgtcgcggc ccagtgatgg cgtgtagtga aagataagga
    13321 tatagaagcc caagttcatg aagacccatg acaaggacga gatgaactcg atgagaaaat
    13381 tggcgcgaaa ggtcatgtcc cgaatgaggc tgtttcgggc aaaggtggcg aagatctgca
    13441 agtaccgtaa aggctgcggc acggtttagc ctccaaaacc actatagtgc cgaagtccac
    13501 gacgaagcgc cacttgcgca aggaaggcga atacgatgac ccaacctgcc tggaccaata
    13561 acccggtcac taaggccgct ccctgaacct tgccaagaaa gaccgccgcc ggaaaatagg
    13621 cgaggtactg aaggggtatc agtttcacca ccgtggccat gggctccggc agcatgtcaa
    13681 tcggaaacat gtgccccgaa aaaaagaagc tcagaagcat atagatgaac aagaaagaac
    13741 tgacctcgag gaaccagaag ccaagaagtc cgagcgtcgc ttccaagaag aaaccaagca
    13801 gaaatgccaa aacaagcgac gcaatatacg tcgcccaaac aagcccatgc ggtgtttcct
    13861 gaaagaaacc ccgacacaga taaaagacca ccgcaaacgg gacgatcgcc acgctgtagt
    13921 acacgagctt gtgagccagg cgatagaaga aaaggtattc gagcagccca atcggctgga
    13981 cgatgaaccg cttgatcgtt ccttcccgaa tttcgcgggt aatgcccgac gccaagcccg
    14041 gcatgctcga aaacgcccga ctgagcatcg tcaacaagta gtaggccacc atgtcgtggt
    14101 aactgtagcc gacaatccgt ccttcactac cgttaccagt gggcaaggct gagaagacgg
    14161 caccccagag gaagatctgt gtgatgatgg ggagaaaccg catgagcgtc cccagcatga
    14221 aatcccctcg ataaataatt cgctcctgga tactgatccg cagaatcgtg atccataagc
    14281 gccaccggta aagcggtgcg gaaatggtat cgatgatagc ggggctgggg tacgtcataa
    14341 gtcgcttagg ttcaggacca ggagagcgtg ctatcaatcc tatgctagtg atttgtcgag
    14401 tgggtcgtgt cttccggtac taccgaattt gcccgttcct gggataaccg agaatttctc
    14461 gcggaggcaa acagggcggc gatcagttcc tcaaggggcg ggtcttccac gctaacatcc
    14521 tccgcgatat atcgggcaag gacagcccga atgacttctg tcgcctcgtt gcgcgcaacc
    14581 cgcagcctaa cccgcggtgg ttcccgaagg atgatttttc caaatcggga caaatcggtg
    14641 ggaacctgtc catcggaaaa ccgtattgtg atgattttgt aatcccccag gtgatccaca
    14701 aggttggtta gggaaccatc ataaataatt tgtccatcaa aaatgacaac aactcgccga
    14761 cacagtacga ccacgtcctt catgtagtgg cttgtcagaa tgacggtcgt cccggtccgg
    14821 gcctgatatt cccgcaagaa ctgctggatc gtgtactggg ccactacgtc caggccgatc
    14881 gtcggctcgt cgagaaacag gacttggggc gagtgcaaca gggcggccac caattccatc
    14941 ttcatgcgct cccccaggga taactcccgc accggacggt gaataaggtg acgaaccccg
    15001 agcatctccg tcaggtcatc gcgcactcgg gtgaactggt ctggcgggat gccgtaaatc
    15061 tgttggtgaa ggtggaacga ttcagccgct ggcaggtccc accacaattg gttcttctgt
    15121 cccatcacca gggcaaagcg ccgtcgataa gcgttctccc gtcgccaggg gatgtggcca
    15181 aggacgcggg cctcgcccga ggtgggcgta atgattcccg aaagaagctt gagggttgtt
    15241 gtctttcccg ccccatttgg cccgaggaac gcaacgaact cgccgctttc gatcgtgaga
    15301 tcaattgacc gaaccgcgtg cacgtattca tactcgggac ggaataacgc ccggagagtg
    15361 gccacaagcc cctctttctt gcgggtaaca cggtatgtct ttgataatcc cctaatttcg
    15421 atggcagcca tactgtcgat tataggacgc gggaaaaacc gtcggaactc atttcggttg
    15481 gaccatcagt cacagcacac attggaagac gacaccgccg atggctttac acaccccaag
    15541 tctcttgaga agtgatcccg tgttggataa tgtggagttt tcgttgcttt tccatggaat
    15601 cgtatctgtg tgagcttggt ttggctggag aggaccaccc ggcaaaccaa gcccagtgga
    15661 taaggcgagg gacgtttcat gcccccacat ggtcagcgtg agacttggac aacccgcatt
    15721 ggcgtgatcc tggctgtcac aggaagtgcc gtcggactgg gcaacttttt gcgattcccc
    15781 gggctagccg ctcaatacgg cggtggcgcg ttcatggtag cctacctctg cgcgtttctt
    15841 tttcttgggc tgcccattgc ctttgccgaa tgggccatgg ggcggtatgg cgggatgcac
    15901 ggccacaact catcaccagg catttatcga gtcatttgga aaagccgggc agcgccctac
    15961 tttggcattt tggctatgat cgtcccggtc ggcatctaca tgtattacgt gtatatcgaa
    16021 agttggtgcc tggcctatgc ctgccggtat ttagccgggg cgatgaattt cggcagcgat
    16081 gcggcccgat atcgggaatt ctttgagcat ttcgtgggga tacacaacca cggagagtcc
    16141 ctatccaccc cgggagaagg gcttctcggt tcggctgttt tctttctggt cgtgtgcttt
    16201 gtgttcaact ttattttgat ctatcggggg ttaagcaagg ggattgaatg gttttgccgc
    16261 tgggccatgc ccgccctagt gatctgtgcc atcatcatcc taattcgggt gttaacgttg
    16321 gggacacccg acccgtcaaa gccggagcaa aacgtcctca gcggccttgg ttacatgtgg
    16381 aaccccgtca aacccggtat gtcgttctgg caggcacttg ccgatcccga aatgtggatg
    16441 aaggcggcca gtcaaatttt cttctccctc tccgtgggtt tcgggatcat catcacttat
    16501 gcaagttatg tcaaaaaaga tgacgatatt gccctgtcga gccttactgc cgcagcggga
    16561 aacgagttct gcgaagtggt cctaggtggg cttatggttg ttcccgccgc gttcgtcttt
    16621 ctcggtcccc aaatcggcca acaattgggt aacccgtttg ccctcggatt catggcactc
    16681 cccagcgttt tcaatcagat gccggtgggc tggctctttg gatgtctgtt cttcttcttg
    16741 ctattcctcg ctgcgatcac cagttccctt tccatgttgc aaccggcggt tgccctcctc
    16801 gaagaggggc tggggctaaa ccgtcgggcc tcggttgcgg tgctgagctt catcacggca
    16861 ataggctcgg caatggttgt ctatttttca aaggatttcg tcgccttgga tacgctggac
    16921 ttctgggtcg ccacgtttgc aatttacttg ctcgc
//
LOCUS       DAOSUT010000085        16826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2114_length_16826_cov_9.805080, whole genome shotgun sequence.
ACCESSION   DAOSUT010000085 DAOSUT010000000
VERSION     DAOSUT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16826
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_16826_cov_9.805080"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..820
                     /locus_tag="PLD05_09245"
     CDS             440..820
                     /locus_tag="PLD05_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOP77666.1"
                     /translation="MSDSTTKKPLGEFRHIDAYDVDDVTVVHFKESRITDDLGIQELG
                     QELFSLVDGANRTKLLLNFANVQFLSSGALGKLRALENRVSKRGGVVKLCSINPNIYE
                     VFKITRFDTIFEIFQTEADALASF"
     gene            1022..4033
                     /locus_tag="PLD05_09250"
     CDS             1022..4033
                     /locus_tag="PLD05_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOP77667.1"
                     /translation="MPLRMMTIVAGLAILWTVLAVAGDPGSLPPGFRNAPGGGPLSNQ
                     NGGLSEGPAPADEGGVEEELVVEVRVVGAKNLPREKYLPSIKTRAGRPFDVNLLEEDV
                     RRLYRTGLFVDIKTYTPKVPGGRLVVFEVLERPLLHYVKFAGQRAFSEKRLAKEAGLK
                     VGDPLDTWQVDEAQRKLEKFYHEKGFSNARVTVIEGNKTTDRGAIFLINEGRRQRVAW
                     TGFVGNTIATDARLRTQVQTKPGWFWFLGGLFNRQQLDDDVERLTAYYRGLGFFRARI
                     GRNIEVYPDPLDDRREWVYVTYVIDEGPRYKVRDIRFFGNQKFSSEELTRILELKPGD
                     YFDQNKLQTDLNKMREKYGSIGHVFADIEPDPRFLEEPGELDLVYRIEEGHPYRVGMI
                     NVRIKGESPHTQVTTVLNRMSLAPGDIADIREIRASERRLRASQIFEVDPSRGAQPKI
                     AFSPPEIPVIAEESQGPRIAERPDRVVPASGTSPAQPEPSSNTTPSPDVFRGQDSGGG
                     ITLPSPPGWVFPNPFGRKPDTASSSSVIPAVGTMPSASAPQNVYGAVYQAPPNRSSNT
                     TLVANESTDVVTFPGGGGNVAVGAGEVTISDMNRGASPTSATVPQMAPDAQGRLVFPD
                     RPMPGDASVFSDRPPDEIPLELPLEPQVEEARTGRLLFSVGVNSDAGVMGSAIIEEQN
                     FDWTRWPRSWRDIIEGRAWRGAGQRFRLEAVPGTRVHRYMVSFSDPYFMNRNVTFGLS
                     GFYYRRDYYEYLEERIGGRVVFGYQFTHDLSGTVAYRGSDIKISDPISTPWGIPPTIN
                     AAVGTNILHGFEIALTHDTRDNSFLPTEGHFFQIAFEQVIGTYDYPRVELDYRRYFAL
                     RQRPDGSGRHVLGLASRFAVTGDETPVYDHYFAGGTSSLRGFDYRGASPREYGIAVGG
                     HCLLLASAEYMFPITADDMVRGVIFCDTGTVEPTIDSWDQRYRVSLGFGLRLVIPAMG
                     PAPIAFDFGFPISEEPGDDNRVFNFFIGFLR"
     gene            4661..6583
                     /gene="speA"
                     /locus_tag="PLD05_09255"
     CDS             4661..6583
                     /gene="speA"
                     /locus_tag="PLD05_09255"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOP77668.1"
                     /translation="MLQVDLQTRRWTVADAEELYEVQSWGKGYFSVNAAGHVCVHPEK
                     DPQRSIDLKHLVDRLIVRGIQTPVLIRFGGILKHRLGEIKQAFEEAIREHNYKGRYHL
                     VYPIKVNQQRQVVEEILRYGAAYQFGLEAGSKPELLAVIALADNTTPIVCNGFKDARF
                     IETAMLAQKMGRNIFLVVEKYTDLELILHFAKEVGVRPNIGIRVKLAAMGSGKWQSSG
                     GHRSKFGLTVSEILRALRELKEHDMADCFRLLHFHLGSQITNIRHIKTALTEGARVFA
                     DLYKQGAGLQYLDVGGGLGVDYDGSQTTFESSVNYTLQEYANDVVYHIQQVCDDAGVP
                     HPTIFSESGRAVVAYNSVLVFNVLGVADRGENGIPEQIPEDAEQPLHDLRQTYDNLNV
                     RNLLESFHDAQEAMENAINLFNNGYMSLEQRAFAENLYWAICRKIFRLMQHLDYVPEE
                     LEVLDSLLSETYFCNFSLFQSLPDSWAIKQLFPIMPIHRLTEQPTQRGILADITCDSD
                     GKIDRFIDRHDVKRTLPLHVFRGEPYFIGVFLVGAYQEILGDLHNLFGDTNAVHVGLD
                     EDGDVVVETVINGDTVREVLSYVEFDPNTLVVRLRNSLEEAIRRGRIDDRQAGRLLRF
                     YESGLEGYTYLENMPE"
     gene            6674..7444
                     /locus_tag="PLD05_09260"
     CDS             6674..7444
                     /locus_tag="PLD05_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOP77669.1"
                     /translation="MKDSIFGLANKTALVTGASRGIGRAIALAFAQAGAEVFICSRKA
                     ESIEAAAKAIREQTGARVEWMAADVSQRQAVDQLAAEALRRMGKIDILVNNAGWNIPQ
                     AIDEIQDKDWDYLIELNLTNCMRLTRAIVPDMKKRRWGRVIHISSMMAMGSTPKRNAY
                     SATKAALVGMARASALDLGPWNITVNCIAPGPIATEMPMTLLSKEQQEQMTQRTALGR
                     WGQPEEIAWPTLFLASEAARYITGQVIVVDGGCLARII"
     gene            complement(7550..9580)
                     /locus_tag="PLD05_09265"
     CDS             complement(7550..9580)
                     /locus_tag="PLD05_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOP77670.1"
                     /translation="MYQSSQTPLWFSLFIFPALGLLFAGCQPAKPVEKVGQQAGTGES
                     VAAPNEATAKAELADGGDKDASPDRTTVGQLWPSQTPSGTPWELPPSPGAPTANETVQ
                     SGWIVPDEPFSTTPSAGGQSQPVESPRLPSSLPGVSPRPETASATQDEAGPLPNPLRG
                     NQVPTAGDATIPQVMPNAPSSPLPAASDTQATPPRVATSKTSDRRLERPPFDPIKENG
                     QFFVGWTKPTVALVFTGNQDGYFEPCGCAGKERMKGGLSRRHTMIEQLRADGWPLVVM
                     DLGGLIKGFGKQTEVKFQITVDALRRIGYHAINLGRNDLRLPAPLLLSVIAPSSGTES
                     EFVSANVALFDFDTDQWTSRFRVVEAGGKRVGVTAILGKSYQEEIRNPDLSFADPAEA
                     LAKVVPQLKQQADILVLLAYAARDEAIALVREFPEFAVVAISDGPAEPPGAPQFVPDT
                     QTMLVFVGEKGMAAAVVGIYERNNPPMAYQRVILDSRYPDSPAMKEMMATYQAQLQAL
                     GLEGLEIRPAPHPRREVQGEFVGSEKCAACHEESYRVWKRSGHSRAWETLVRLDPPRN
                     FDPECISCHVTGWHPTRYFPYLSGFLSEEKTPHLINVGCESCHGPGGEHVAAEMGSDL
                     ERQRRAAEAMIVTKEQAESSEAHSCRNCHDLDNSPDFDFSTYWPLVEHYEKE"
     gene            complement(9646..10845)
                     /locus_tag="PLD05_09270"
     CDS             complement(9646..10845)
                     /locus_tag="PLD05_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOP77671.1"
                     /translation="MKRSVWAALIIFPILLGVIFGGVLGWWSVKRRPWPGYPILAEAQ
                     PAHSGARSEAGATSSEPLPKIDVPEDTYDFGRIESNSVVRHEFTVRNVGTAPLRISIG
                     DSTCRCTVLNLDSAEIPPGESKPVTLEFNGKDYFGPVTQRATILTNDPTRPQVTFTIK
                     ADVVKSIRVVPSEIVFSRVVKMQPAMGEAKIYVFRDQPVQVKGIELANKDENAEFIDL
                     DIQPLAPDELTVEPGAKSGYRVTVRLKPGLPLGPIQQKIRVLTDQKDMPELEIPLAGR
                     VESDVNITGVGWDSSRGMLHLGRINRSSGAERRVMLRLTPLVGENLKVEVDRTIPDFL
                     QVTIEEPRYVEASLSLFVPITIRVPPGAGPANYFGLDEAHAGKVILKTNHPDAPEVKI
                     LVRFAVD"
     gene            11424..12305
                     /locus_tag="PLD05_09275"
     CDS             11424..12305
                     /locus_tag="PLD05_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77672.1"
                     /translation="MKRQAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCVNN
                     LRQLGLGFQNYHDVWGQFPLPGMIANQLGWTASILAFIEQKPIYDQMDWNAGNYMDAG
                     KIKHAANRIPVYLCPSGDQERTEYASEIWPNPGGQRVYTVHYYGILGPYGTNPTTNQT
                     YKCRNTTEAFGGECDQGVMWQFSSRMADITDGTSNTLLLGEISWSQMTKYRAWTRGKF
                     QDSRGTLYLLSKYLRFPINSKNESIWNGIAFGSFHPGGCQFVNVDGSARFVSETIDFG
                     IYLALGSKDGGEALQSQ"
     gene            12358..12813
                     /locus_tag="PLD05_09280"
     CDS             12358..12813
                     /locus_tag="PLD05_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77673.1"
                     /translation="MVSFQRVAILFLFATVGCLLVCSQGCGKKDPRKNAIWGTVTYRG
                     QPVPAGSITFIPDASQGNSGPAVTIPFENGRYDSRSSGLGHIGGPHKVRITGLTGKDS
                     GDEFFPQGTLLFPDYETSANLPDGPSEQNFVVPDNWVLPPSTPQRYEGP"
     gene            13445..14302
                     /locus_tag="PLD05_09285"
     CDS             13445..14302
                     /locus_tag="PLD05_09285"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOP77674.1"
                     /translation="MSDKISEQSRPNSDLASARFIVDIDLSDFPAVRESEYPYNPTAD
                     QLERLKKVSEILEEAPAEVILRWAIDTFYPELTMATAFGPEGCVILSILARLQPNVRV
                     FNLDTGYQFRETLELRDRIAAKYGIQVVLARPELSVEEYEKRNGGPVYKSDPDRCCFD
                     RKIQVLKREVVGYRAWISGIRRDQSPHRANAPIVGWDKKFGLVKINPLARWTKSQVWK
                     RIMDENIPYNPLHDQGYPSIGCWPCTRPVLSGEDERAGRWSGTAKTECGLHLVDEQPL
                     PPPTTYGQL"
     gene            14378..14815
                     /locus_tag="PLD05_09290"
     CDS             14378..14815
                     /locus_tag="PLD05_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOP77675.1"
                     /translation="MKISAKTEYACIAILELASCYETGGPVQVRKIAERHAVPSRFLV
                     QIMLQLKAAGLVASTRGAAGGYRLAVPPEEITLGQVMDIIEGNQPSETGFSLSGSPDS
                     PAVRVLAQTWQEVQEKEKEMLSSITFAELLERARQYESQMYYI"
     gene            15091..>16826
                     /locus_tag="PLD05_09295"
     CDS             15091..>16826
                     /locus_tag="PLD05_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOP77676.1"
                     /translation="MNSPWRYALCCAGLATIVFFFNLGGPKLWDRDEPRNAGCAREMI
                     ERHDWVTPYFNGELRDHKPVLLYWFMMSAYAVFGVNEFAARFWSAVLGVGTVLLTFDL
                     GRRWFGPRTGFWAGVMLSTCMMFPVAARAATPDSVLIFFSTAALWLYAGPLLESAQSC
                     VSPGNPAAKRSRFWQIVESYALTGLAVLAKGPIGVVLPVGIIFLFHLWRDEPTDAAPR
                     PSTQGVFRKFLSFLHPQRIWAVIRRMNLGLGILVVMLVAAPWYVWVGLRTDGAWLRGF
                     FLTHNVNRFLEPMEGHRGPLFFYPLVFFASFFPWSFLIIPAVPSFIRSLRSRDSTSEK
                     LAFLTVWIAVYIGFFSLAGTKLPNYITPCFPAFALMLSWWLDRWLSGLEAEAKGLLAG
                     VTVVYAVVGVVLSIALPVAATRFLPGEEVLGTLGVIWLVGSVVAMLALGRGQRIGYAG
                     ALATTASLFALGAFGVAAARVSRHQQIDELTACLKSLTPNPHVASYDCLEPSWVFYLG
                     RPITFIPASNTQELVDFLGKGDSACVLTTRDRYERIRPCIPADVAVVAEIPYFLRDKQ
                     LVLLGGKKVLAR"
BASE COUNT         3520 a         4556 c         4912 g         3838 t
ORIGIN      
        1 gattccgacg ttcgcttgcc ctcaccccaa ccctctcccg gagggagagg ggactttttg
       61 agataggctc taaactcacg tgacggctgc cgtgtactgc caaagtcgga aggacgtgcg
      121 ccgtctcttc cgctgaagag ccgatttgta atcggttgtc gttcccaggg acaatggcac
      181 gtgggcctct agtaatccgg cgagaggaac gcggtgttac cggggtgacg atccgcggga
      241 aaaagtaggg ccacttgaat taagggattt tgacagtatg atggcgcaag tgtcatgtga
      301 ggcaggctcg cccgggaaaa aataaaaaat cgtcgcactg gttttctgtg acctctgcga
      361 acgacgacag ggcgggtttc gggggaaccg ggtggacctc ggtgaccggc agggcttatc
      421 gtcgagacga ggatcaacca tgtcagattc gactacaaag aagccgctgg gtgagtttcg
      481 ccatatcgat gcgtacgatg tggacgatgt gacggtcgtt catttcaagg aaagccggat
      541 caccgacgac ctgggcatcc aggagttggg ccaagagttg ttctcgttgg tggacggagc
      601 caatcgaaca aaactcttgc tgaattttgc caatgtgcag tttctgtcaa gcggtgcttt
      661 gggaaagctg cgggctttag agaatcgcgt ttccaagcgg ggtggggttg tcaagctttg
      721 ttcgatcaat cccaacattt acgaagtctt caaaattacc cgcttcgaca caattttcga
      781 gatctttcaa acagaagcag acgccctcgc cagtttctga ggaaattggc agtaagtgtt
      841 gattctcccc caagcaggct gtggcctttt cccgccggat agtcgcttct tcgcgaaact
      901 cgtcttgtca ctcaccccaa tatcgggtaa ttgttccgcg gggcggcgcc ctttactttg
      961 cgccgcgtgt ttaaccacga tccattgccg ccgggacaag aaagcgcacg atctctgcct
     1021 tatgccgtta cgcatgatga caatcgttgc cgggctggca atcctctgga cggtgctggc
     1081 cgttgcgggg gacccaggct cgctgccgcc gggtttccga aacgctcccg gaggggggcc
     1141 tctttcgaat caaaacggtg gcctctccga ggggccggcg ccggcggacg aaggcggggt
     1201 cgaggaagaa cttgtcgttg aggtgcgggt cgtgggggcc aaaaatctgc cgcgggaaaa
     1261 gtatctgccg tccatcaaga cccgtgcggg acggccattt gatgtcaacc ttctggaaga
     1321 agatgtccgc cgcctttatc gcaccgggct tttcgtggat atcaagacct acacgccaaa
     1381 ggtgcccggg ggtcggctag ttgtgttcga agtcttggaa agaccacttc tgcactatgt
     1441 gaagtttgcc ggccagcgag ccttcagcga aaagcggctg gccaaggagg ccggcctgaa
     1501 ggtcggtgac ccgctcgaca cctggcaggt tgacgaagcc cagcgaaaat tggagaagtt
     1561 ttatcacgag aagggcttct ccaacgcgag ggtcacggtg attgaaggga ataagaccac
     1621 tgaccgcggg gccattttcc tgatcaatga aggtcggcgg cagcgtgtgg cttggacagg
     1681 ctttgtggga aacaccatcg ccacggatgc ccgactgaga acccaggtcc agaccaaacc
     1741 cggttggttt tggttcttgg gcggattatt caatcgccaa cagcttgatg acgatgtgga
     1801 acgcctcacg gcctactacc gtggtttagg gtttttccgg gcgaggattg ggcgtaatat
     1861 cgaagtttat cccgacccgc tggatgaccg ccgggagtgg gtctatgtga cgtatgtcat
     1921 cgatgaaggt cctcgctaca aggtgcggga catccgcttt tttggaaacc aaaagttttc
     1981 cagtgaagaa ctgacccgca tcttggagct gaagcctggg gattacttcg atcaaaataa
     2041 actccagact gacctcaata agatgcggga aaaatacggg tcgatcggcc acgtcttcgc
     2101 tgatattgaa cctgatccgc ggtttctcga ggagccgggc gaactcgacc tggtgtaccg
     2161 aatcgaggag gggcacccct accgagtcgg aatgataaac gtgcggatca aaggggaatc
     2221 tccccacacc caggtcacga ccgtgctcaa tcggatgtca ctggccccgg gcgatattgc
     2281 ggatatccgc gagattcgcg cgagcgaacg gcggttgcgg gcatctcaaa tttttgaggt
     2341 ggatccctca cggggcgcac agcctaagat cgccttcagt ccacctgaaa tccctgtgat
     2401 tgccgaggag tcccaagggc cgcggatcgc cgaacgtccg gatcgcgtcg tgcctgcctc
     2461 gggaacgtcg cctgcgcagc cggaaccgag ttccaacacc acgccctcgc cagacgtttt
     2521 tcggggccag gacagtggcg ggggtatcac actgccctca ccccccggat gggtctttcc
     2581 caatcctttt ggacgtaaac ccgataccgc cagttcttcg tcggttattc cggccgttgg
     2641 tacaatgcca tccgcatcgg caccgcagaa tgtttacggt gctgtttatc aagcgccgcc
     2701 aaaccgttca tccaatacca ccctggtagc caacgaaagc accgacgtgg tcactttccc
     2761 cgggggtgga ggcaacgtag ctgtgggggc tggcgaggtc acgatttccg acatgaatcg
     2821 gggcgcctcg cccacgtcgg caacagtccc acaaatggca ccggacgcgc aaggccgtct
     2881 cgtgtttccc gatcgcccta tgccggggga tgcctcggtg ttttccgacc ggccaccgga
     2941 cgaaattccg ctcgagctgc ccttggagcc gcaagtggaa gaggcccgaa ccggccggtt
     3001 gttgttcagt gtgggggtca actcggatgc cggggttatg ggcagcgcga tcatcgagga
     3061 acagaacttt gattggacgc ggtggccacg gagctggcga gacattattg aaggcagggc
     3121 ctggcggggt gccggtcagc ggttccggtt ggaagccgtc cccggcacca gggttcaccg
     3181 ctacatggtc agcttttcag acccctattt catgaatcga aatgtgactt ttggtttgag
     3241 tggtttttac tatcgccgag actattatga gtatctcgaa gagcggatcg gtggccgggt
     3301 cgtcttcggc tatcaattta cacacgacct ttcgggaacc gttgcttacc gcggctcgga
     3361 tatcaagatt tctgacccga tttctacgcc ctggggaatc cctccaacga tcaacgcggc
     3421 cgtgggaacg aacatccttc acgggttcga aattgccttg acgcacgaca cccgcgacaa
     3481 ctcgttcctg cctaccgaag gtcacttttt ccagatcgcc tttgaacagg taattggcac
     3541 atacgattac ccccgtgtgg aattggacta tcgccgctat tttgccctgc ggcaacgccc
     3601 ggatgggtcg ggacggcacg tccttggctt ggccagccgc tttgccgtca caggagacga
     3661 aacaccggtg tacgaccact attttgccgg gggtacgtcg agcctccgag gcttcgatta
     3721 tcgcggggcg tccccccgag aatatggtat cgcggttggc ggacactgcc tccttttggc
     3781 ctcggcggag tacatgttcc ccatcacagc cgatgatatg gtccgcgggg tgatcttctg
     3841 cgacaccggt acggtcgagc caaccattga ctcttgggac cagcgatatc gggtatccct
     3901 gggttttggg ttgcggctgg tcatcccggc gatgggaccg gccccgattg cctttgactt
     3961 cggcttcccg atcagcgaag aacccggcga cgacaaccgc gtgttcaact tcttcatcgg
     4021 cttcttgcgg taaaaagcgg tcagtccact tccaccggaa aactcccgtc gttgcctcga
     4081 ctttgagacg acaaagaccg ctgtcatttt ttatgagagg ggtaccagcc caaaacccgc
     4141 gtgatgtcca ctacaaactc aggggaaggt ggggggatga taatcgacag actcgcttca
     4201 gtcgaaccgc gatgccgtat ctctgaggtc gcgggactgc tggagcagag ataaatcgtc
     4261 tacgaatttc tgggctttcc aacccccttt tctcctggcc gcaatttaat acgatacggt
     4321 gataaggaaa aacaacaaaa taggagtgga aacgtagtac aaagagccca tcccaaaaag
     4381 tcgcctctcc cttcgggaga gggttggggt gagggtaagc ggacgtcgga atcgattgag
     4441 aaaacccgca gcgattcgag gcaccgtcac ccccgacccc tctcccgccg aacgggcgag
     4501 gggagattat gggatggact caaaaggggc cagggagagg tggtggccca gtggggaaga
     4561 ggaagctcgg ttggtgggtt tgtgttaacc gcggcctgct ggacggacgt tgtcattagt
     4621 ggaaaagcgt tttgccagtg gcagaggggg gacaataccc atgctacaag tcgatctgca
     4681 gacacggcgc tggacggttg ccgatgccga ggaactctac gaggtccaga gctggggcaa
     4741 ggggtatttc tccgtgaacg cggcggggca cgtatgtgtc catccggaga aggatcccca
     4801 gcgatcgatc gatttgaaac atttggtcga tcgcctgatc gttcgtggga tccaaacgcc
     4861 cgtgcttatc cgtttcggcg gtatcttgaa gcaccgtttg ggcgagatca agcaggcctt
     4921 tgaggaggcc atccgagaac acaactacaa aggccgctat cacttggtct atccaattaa
     4981 ggtcaatcag cagcggcagg tcgtcgagga gattctccgt tacggggcgg cttatcagtt
     5041 tgggcttgaa gccggcagca aaccggaact cttggccgtc attgccctgg ccgacaacac
     5101 aactcccatc gtttgcaacg gcttcaaaga tgcccgtttc atcgagacag ccatgctggc
     5161 ccaaaagatg ggacgcaaca tcttcttggt tgtcgaaaag tacaccgatt tggagctaat
     5221 tctccatttt gcaaaagagg tgggagttcg gcctaacatc gggattcgtg tcaaactggc
     5281 ggccatgggg tccggcaagt ggcaatcctc aggggggcac cgctcgaagt ttgggcttac
     5341 ggtcagcgaa attctccggg ctttgcgcga gctcaaagag cacgacatgg ccgattgttt
     5401 caggttgttg catttccacc tgggcagcca gatcacaaat attcggcaca tcaagacggc
     5461 cctcacggaa ggtgccaggg tcttcgcgga cctctacaag caaggtgctg ggttgcagta
     5521 cctcgatgtc ggtggcggcc ttggcgtcga ttacgacggc tcgcaaacga cgttcgaatc
     5581 cagcgttaac tacaccttgc aggaatatgc caacgacgtt gtgtatcaca ttcagcaggt
     5641 ctgcgatgat gcaggcgtgc cccatccgac gattttttcc gaaagtggtc gggccgttgt
     5701 ggcctacaac agtgtgctgg tgttcaatgt gcttggcgtt gccgaccggg gtgaaaacgg
     5761 catcccagag cagattcccg aggatgccga acagcctctc catgatctcc gacaaactta
     5821 cgacaacttg aatgtacgaa atctcttgga gagcttccac gacgcccagg aggccatgga
     5881 aaatgcgatc aatctgttca acaacggtta catgtcgttg gagcaacggg catttgcaga
     5941 gaatctttac tgggcaattt gccgcaagat cttccgcctc atgcagcatt tggattatgt
     6001 cccggaggaa ctcgaagttc tcgattccct attgagtgag acatatttct gtaacttttc
     6061 actgtttcaa tcgttgcccg atagttgggc gattaagcag ctctttccga tcatgcctat
     6121 ccatcgtctc acagagcagc ccacacagcg ggggattttg gccgatatta cgtgcgactc
     6181 cgatgggaag attgaccgct tcattgatcg ccacgatgtg aagcggactt tgcccctcca
     6241 tgtatttcgc ggtgagccct acttcattgg cgtatttctg gtaggggctt accaggaaat
     6301 cctcggcgac cttcacaatc tgtttgggga tacgaatgcg gttcacgtgg gactggacga
     6361 agacggcgac gtggtggtgg agaccgtcat caacggtgat accgtgcgcg aggtcctatc
     6421 ctacgtggag ttcgatccca acacgttggt cgtgcgtttg cgaaatagcc ttgaagaggc
     6481 cattcggcgg ggacgcatcg acgatcgtca ggccgggcgg ctgctccgct tctatgagtc
     6541 ggggctcgaa ggctacacgt acttggagaa catgcccgag tgatggcggc gccacgtcga
     6601 aaccgcgtta cgcagcgaag attttgagcg aatcctaggc aggcttcatt tgattgcacg
     6661 tgacggtgac agcatgaaag atagcatctt tggcttggcg aataagacgg cacttgtgac
     6721 gggagcaagt cgagggattg ggcgtgccat tgccttggct tttgctcagg ccggggcgga
     6781 agtcttcatt tgcagtcgca aggcggagag cattgaggcc gcggcaaagg cgattcgtga
     6841 gcaaacgggg gctcgggtgg agtggatggc cgccgacgtt tcgcagcgcc aggccgtgga
     6901 ccaactggcc gccgaggccc ttcggaggat gggcaaaatt gacattctgg tcaacaatgc
     6961 gggctggaat attccccagg ccattgacga aatccaagac aaggactggg attacctgat
     7021 cgaactgaat ctgaccaatt gcatgcggct gacgcgggcc attgttcccg acatgaaaaa
     7081 gcgccgctgg ggtcgcgtga tccatatttc gtccatgatg gcgatgggaa gcacgcccaa
     7141 acgcaatgcg tattctgcca cgaaggcggc gttggtgggc atggctcggg caagtgccct
     7201 tgatcttggc ccgtggaata ttacggtcaa ctgtatcgct cccggtccca ttgccacgga
     7261 aatgccaatg acgctcctca gcaaggagca gcaggagcag atgacccagc gaactgcctt
     7321 gggccgttgg ggacagcccg aggagatcgc ctggccgacg cttttcctgg caagtgaagc
     7381 ggcacgctac attactggtc aggtgattgt tgtcgatggc ggctgcttgg cacgaatcat
     7441 ctgatcatct aaaaaatcca taaaggtccg gcgacaggct accggttagg ccagaatgaa
     7501 ccagtgccag accgccacat ttccacaagg ctacatcacc tcgacgccgt cactcttttt
     7561 cgtagtgttc gactaggggc cagtatgtgg aaaagtcaaa atcggggctg ttatcgaggt
     7621 catgacagtt tcggcacgag tgggcctccg agctttcggc ttgttccttg gtaacgatca
     7681 ttgcctcggc tgcccgtcgt tgccgctcga ggtcacttcc catctcggcg gccacgtgtt
     7741 ctccgcctgg cccatggcac gattcacaac ccacgttgat caggtgtggt gtcttttcct
     7801 cggaaaggaa acccgaaagg taagggaagt accgcgtggg atgccagccc gtcacgtgac
     7861 agctaatgca ttcgggatcg aagttgcgtg gcggatcaag tctcacaagc gtttcccatg
     7921 cccgagagtg gcctgaccgt ttccaaacgc gataggattc ctcgtgacat gcagcacact
     7981 tttcagaccc gacgaactcg ccttgcactt cccgtcgcgg atgcggagcc ggacgtatct
     8041 caagtccctc cagcccaagg gcctgaagtt gtgcctggta ggtggccatc atctctttca
     8101 tggcgggcga gtcgggataa cgagagtcga gaatgacccg ctggtaggcc atcggcgggt
     8161 tgttacgctc gtaaatgcca acgacggccg ccgccatccc cttctcaccg acgaagacga
     8221 gcatcgtttg ggtatcggga acaaattgcg gggcacccgg cggttctgcc ggaccatcag
     8281 aaatggctac cacggcgaac tcgggaaatt cgcggactag ggcgatggcc tcatcccgag
     8341 ccgcatatgc caacaggaca aggatgtcgg cctgttgctt taactggggg acaaccttgg
     8401 cgagggcctc ggcaggatcg gcaaaggata ggtcgggatt gcgaatctcc tcttggtagc
     8461 tctttccgag gattgccgtc acgccaacgc gtttcccccc agcttcgaca acgcggaacc
     8521 ggcttgtcca ctgatcggta tcaaaatcga atagggcaac attggctgaa acgaactcac
     8581 tctccgttcc gctgcttggt gcaataacag acaataacaa cggtgccggt agtcgcaagt
     8641 cgttgcgacc caaattgata gcgtgatacc cgattcgcct tagggcatcg accgtgattt
     8701 gaaacttgac ctctgtctgc ttgccaaagc ctttaatgag acctccaagg tccatgacga
     8761 cgagtggcca accatcggcc cgaagctgtt cgatcatcgt gtgccgtcgg ctcaagccgc
     8821 ctttcatccg ctccttgcca gcgcaaccgc acggctcaaa atatccatct tgattaccgg
     8881 tgaagacaag cgccacggtt ggttttgtcc aaccgacgaa gaattggcca ttctccttga
     8941 tgggatcaaa aggtggccgc tctaaccggc ggtccgaggt cttggatgta gccacccggg
     9001 gtggcgtcgc ttgtgtgtcg gaggccgcag gtagaggcga gctcggagcg ttgggcatca
     9061 cctggggaat ggtagcgtct cccgcggtgg gaacctgatt gccccgcaga ggattgggaa
     9121 gaggccccgc ttcgtcttgg gttgccgaag cagtttctgg cctgggtgaa acaccgggca
     9181 gcgacgatgg cagccgcggg gactccaccg gttgagattg cccaccggct gatggcgtcg
     9241 tggaaaacgg ctcatccggg acgatccagc cactctggac agtttcatta gccgttgggg
     9301 cacctggact gggtggcagc tcccaaggtg tcccgctggg tgtctgcgat ggccacagtt
     9361 gacccacagt tgtccgatcg ggcgaagcat ctttgtcccc accatcggcg agctccgcct
     9421 tggcggttgc ctcattgggg gctgcaacag attctcctgt ccccgcttgc tggccaactt
     9481 tctccactgg ttttgctggc tggcagcctg cgaataacaa tcccagcgcc gggaaaatga
     9541 acagactaaa ccacaagggc gtctgagaag attggtacat ccgtcacgtt ctccgtacaa
     9601 ggggatccgt cctgaaaaat gcacgcccgt tcgcaagccg tgcactcaat ccacggcaaa
     9661 gcgaacgagg atctttacct caggcgcatc cggatggttg gttttaagga tcacttttcc
     9721 cgcatgtgct tcgtccaaac cgaagtaatt agccggcccg gccccgggtg gaacacgaat
     9781 cgtgatggga acgaacaaac tcagcgaggc ctccacgtac cggggttcct caattgttac
     9841 ttgtaagaaa tcaggaattg tacgatcgac ctcgaccttt aggttttccc caacgagagg
     9901 tgtcaggcgg agcatcactc gccgttccgc ccctgagctg cgattgatcc gcccaaggtg
     9961 taacatcccg cgggaactat cccagccaac acccgtgatg ttgacatcgc tttccacccg
    10021 accagccaat ggaatctcca actcaggcat atccttttgg tcggtcaaca cgcggatttt
    10081 ctgctgaatt ggccctaaag gcaagcctgg ttttagccga accgtcacac ggtagcccga
    10141 tttggcccca ggttccacag taagttcgtc gggggcaagg ggctgaatat ccagatcaat
    10201 aaactcagcg ttctcgtcct tgtttgccaa ctcaatccct ttgacctgga cgggctggtc
    10261 ccggaataca taaatctttg cttcccccat tgccggctgc atcttgacca cccgactgaa
    10321 gacgatctcg ctcggcacga cccggatcga tttcacgaca tcggccttga tcgtaaaagt
    10381 gacctgcggt cgggtggggt catttgtcag aatggtggca cgttgtgtga ccgggccaaa
    10441 atagtccttg ccgttaaatt ccagcgttac tggctttgat tcgcccgggg ggatctctgc
    10501 cgaatcgagg tttagcaccg tgcagcgaca agtactgtca ccaatggaaa ttcggagggg
    10561 agccgttccc acgttccgca cggtgaattc gtgcctcacc acagagttcg actcaatccg
    10621 accgaagtcg taggtatctt ccggaacgtc gatcttgggg aggggctcag acgaagtggc
    10681 tcccgcctca ctcctcgccc cggaatgggc tggctgggcc tccgccaaga tggggtaacc
    10741 aggccacggc cgccgcttaa cactccacca gccaagcact ccgccaaaaa tgacacctaa
    10801 caagatcgga aaaatgatca atgcagccca aacagaccgc ttcatggcgt catgctccgc
    10861 ttcaatggtt gtatcattcc gccgctcatt gccgctggaa aaccacggtc atgttctacc
    10921 attttacata cgcagccact aaaagggggt tttgggccga tacctttttg ggctcggtgc
    10981 gaaacggcct gacacgcctt ttgtgaccag tgttgtgtgt ggtgctaacc gaaagaatgc
    11041 tgctttcact gaaagccgcc ctcgaactag tattttttcc aaaagcattc gacaattccc
    11101 gcggcggacc gggtcacgct ctttgttgac atctcctcta ggtgcgttta tcatcgaagg
    11161 atgcctggga gaaaggggaa tagtgacctg cacttttgtc actaactcgc acccacccgg
    11221 cggtattccc ttcccgcgtg tgctcacgct gtgcaggtga ggcacattga tcgtgcccgt
    11281 cgggcataag agggagtggt tccctgctct cggagcgacg ttcaccgact acgaacagtg
    11341 ccggagggag tgtttttaag gagtgttttt gtcgttttgt ttgccgggtt tcgaacgatc
    11401 ctgcattcct gaggatgttt ttcatgaagc gtcaagcgtt tacgttggta gaactattgg
    11461 ttgttattgc gattattggc atcctcatcg cgcttttgct gccggcggtc caggcggcgc
    11521 gcgaggcagc ccgacggtcc cagtgcgtta ataacctaag gcagcttggc cttggttttc
    11581 aaaactacca tgacgtgtgg ggacagtttc ccttgcccgg tatgattgcc aatcaactgg
    11641 ggtggacagc ctcgatcctg gccttcattg aacaaaagcc gatttacgac cagatggact
    11701 ggaacgcggg caactacatg gatgccggta agatcaagca tgcggccaat cggattcccg
    11761 tgtatttgtg tccttcagga gatcaggaac gcacggaata tgcctccgag atctggccca
    11821 accccggtgg ccaaagggta tacacggttc actattatgg cattctcggc ccatacggga
    11881 ccaatccgac gaccaatcaa acgtacaaat gccgtaacac gaccgaagcc tttggtggcg
    11941 agtgtgatca gggcgttatg tggcaatttt ccagccgaat ggccgatatc actgacggaa
    12001 cttcaaatac gctcctcttg ggcgagattt cctggtcgca aatgacaaag taccgcgctt
    12061 ggacgcgtgg caaattccag gacagccggg gaaccctcta cttgctatca aaatatcttc
    12121 ggttccccat caattccaag aatgaatcga tctggaatgg tatcgccttc ggcagcttcc
    12181 atcccggagg atgtcagttc gtcaacgttg atggttccgc acgattcgtc tcggaaacga
    12241 tcgacttcgg gatctatctt gctttgggca gcaaggatgg aggagaggcg ctccagtcgc
    12301 aatgaccgtg gcactcagaa acttctttga tatatcggca gcaaagggca cgacgtgatg
    12361 gtttcctttc agcgagtcgc tatcctgttc ttgtttgcga cagttgggtg tcttttggtt
    12421 tgctcacaag gctgcggaaa gaaggatcct cgtaagaacg cgatctgggg gactgtgact
    12481 taccgaggcc agccggtccc ggcaggttcc atcacgttta ttcccgacgc ctcccaggga
    12541 aactctggtc cggccgtgac gattcccttc gaaaacgggc gttatgactc gcggagcagc
    12601 gggttagggc acatcggcgg acctcataag gtccgtatca cgggcctaac gggcaaggat
    12661 tctggggatg agtttttccc tcaagggacg ctcctgtttc ccgattacga gacgtccgcg
    12721 aatttacccg acggacccag tgaacaaaac tttgttgtgc ccgacaattg ggtactaccg
    12781 ccatcgacgc cgcagcgcta tgaggggcca tagataccga cggccatcaa tggggcgggg
    12841 agaacgttac tggggtttgc atcgggtcat aggaaagcgt gtccgccaga ccttggggcg
    12901 cactcggccc acgctggttt tttatcgggg gttgccaggg tcgagaatgc gcgggttcct
    12961 agcgaagaaa gctggcggca cagttgatgc tcgcccgtgc gcgaaaccgc actcaccaag
    13021 tcgttacgat ttataagagc taccttctgc cttttcattt tttggcggcg ttgaccctcg
    13081 gtgaagtctc agccctattt ggggattctt tcggggtggc agggctttcg cgagatcttg
    13141 aaccggctcg ctagttcgcg aagaggcgcc cgggtggaga gcttttcccc ggctggaagc
    13201 caactcctca atgccgtgga ccgactgcgc tagggccaga gccacctttt gcctggctgc
    13261 tcgaaaagga ggggacagcg tgccaaatgc ttcagcgaga aatgccgata ttgcctaata
    13321 gagataattg acttagttga caaggatact tataaataat agaaggtcac gactttggca
    13381 aatggttttg cgaacggtta gcaccatggc gacgatagca ataagcccta cccaaaacag
    13441 acctatgagc gataaaatta gtgaacaatc ccgtcccaat tccgacctgg caagtgcaag
    13501 gtttattgtt gacatcgatt tgagtgattt tcctgcggtc cgggaaagtg agtatccgta
    13561 caatcccacg gcggatcagt tggaacgcct caaaaaggtg agcgaaatcc tggaggaggc
    13621 ccctgccgag gtcatcctcc gttgggcaat cgacacattt taccccgaac tgactatggc
    13681 cacggcgttt ggcccggagg gttgcgtgat cctctccatc ctggcgcggt tacagccgaa
    13741 tgtgagggtc tttaacttgg atacggggta tcaatttcga gaaaccctag aattgcggga
    13801 ccgcattgcc gcgaagtacg ggattcaggt ggtcctagct cgcccggagc tgtcggtgga
    13861 ggaatacgaa aagagaaacg gcgggcccgt gtacaagtcc gatcccgatc gctgctgctt
    13921 tgatcggaag attcaggtct tgaagcggga ggtcgtgggc taccgggcgt ggatcagcgg
    13981 catccggcgg gatcaaagtc cgcaccgggc gaatgccccg attgtgggtt gggacaaaaa
    14041 gtttggcttg gtgaagataa accccctggc acgttggacc aaaagccagg tgtggaaaag
    14101 aatcatggac gaaaacattc cgtataatcc cttacacgac caaggttacc ccagtatcgg
    14161 ctgctggccg tgcacacgtc ctgtcctcag cggcgaagac gagcgggcag ggcgctggag
    14221 cggcacggcc aagaccgaat gcggtttgca cctggtggat gagcagccac tgcctccgcc
    14281 cacgacgtat ggtcagcttt gagtctaagc agcgtaactg ccgcgaaacg gcggcgagtc
    14341 ttcgcaagac aggggcataa agaaggcctg ggtgacaatg aaaatttctg caaaaaccga
    14401 atacgcctgt atcgccatac ttgaactagc ctcgtgctac gagacgggcg ggccagtcca
    14461 ggtccgcaag atcgcggaac gccatgccgt tccttctcgc tttctcgttc agatcatgct
    14521 tcagctcaag gcggcgggac ttgtggccag tactcgcggg gctgccgggg gttaccgttt
    14581 ggccgtccct cccgaggaga tcaccctcgg ccaggtcatg gacatcatcg aaggcaatca
    14641 gccctcggag acaggttttt cgctctctgg gtcgccggac tcccccgccg taagggtcct
    14701 tgcccagacg tggcaagagg tccaagagaa agaaaaggag atgctttcct cgatcacctt
    14761 cgccgaactt ctagagcgtg cccgccagta tgaatcgcag atgtactaca tctgagggat
    14821 gacatctgcg taatggttgt tccggggcgg cggaccacca tgacatcccc ggcttaccat
    14881 actttggata ccttcagtgg ttttcctttc attagagtgg gtctcctttg cgtattgtcg
    14941 cgagaagaag caggaaagct ctaaccaaaa cttgccttgt cgggggcctt tcctagaggt
    15001 atgatcgggc aacgcctgcc cgaatgccaa agggcgtgag aaagtgggca gggtgcgggg
    15061 cctggataac tttgccacgg agggtgcttg atgaattccc cttggcgata cgctctttgt
    15121 tgtgccgggc tggcgacaat cgtattcttc ttcaatctcg gtggtcccaa gttgtgggat
    15181 cgggacgagc cgcgaaacgc cggttgtgcc cgggaaatga ttgagcggca cgattgggtc
    15241 accccgtatt tcaatggtga attgcgggac cacaagcccg tgctgctcta ttggttcatg
    15301 atgtctgcat atgcggtttt tggagtcaac gagtttgccg cgcgtttttg gtccgccgtc
    15361 ctaggtgttg gcaccgtttt attgacgttc gatcttggcc gtcgctggtt cgggcctcgg
    15421 accggctttt gggccggggt catgctcagc acctgcatga tgttccccgt tgccgctcgg
    15481 gctgccactc ccgattcggt actcatcttc ttttccaccg cagcattgtg gctttacgcg
    15541 ggtccactgc tcgaatccgc tcagagttgt gtttcccctg ggaatcccgc cgcaaaaaga
    15601 tcgcgatttt ggcagatcgt tgagagctac gcattgacgg gcttagcggt cctggccaag
    15661 ggccccattg gggtggtgtt gcctgtgggt attatctttc tctttcatct ctggcgagat
    15721 gaacccacgg atgcggcgcc caggccttca actcaaggtg ttttccggaa attccttagt
    15781 tttctccatc cccagcgtat ttgggctgtg atacgccgga tgaacttggg gctgggaatt
    15841 ctcgttgtaa tgttggtggc tgccccatgg tatgtttggg taggattgcg gacggatggg
    15901 gcctggttgc gcggcttttt cctgacgcac aatgttaatc ggttcctaga accaatggag
    15961 ggacaccgcg gcccgttgtt cttctatccg cttgtcttct tcgcttcctt tttcccttgg
    16021 tcgtttttga tcattcctgc tgtcccttct tttatcaggt cgttgcgatc ccgggatagc
    16081 accagtgaaa agctggcatt tttgacggtg tggatcgcgg tttacatcgg tttcttttct
    16141 ctggcgggga ccaagctgcc caattacatc accccgtgtt ttccggcgtt tgccctgatg
    16201 ctgagttggt ggctggaccg ctggctgagc ggcctggagg ccgaggccaa gggcctcctt
    16261 gccggtgtca cggtggttta tgctgtggtg ggagttgtgt tgtcgattgc cctcccggtg
    16321 gcagccactc gttttctccc gggcgaggaa gtccttggga cgttgggggt gatttggctt
    16381 gtgggcagtg ttgtagcgat gttggccctg ggccggggtc agcggatcgg ttacgccggg
    16441 gccttggcga ccaccgcctc gctttttgcc cttggtgcct ttggggtggc ggcggcccgt
    16501 gtatcgcgtc atcagcagat tgacgagctc acggcctgtc tcaaaagcct gacccccaac
    16561 ccacatgtgg cttcatacga ttgcctggag cccagttggg tcttctattt gggccgtccc
    16621 atcaccttta ttccagcatc caacacccag gagcttgttg actttctggg gaaaggcgat
    16681 tccgcttgcg ttttgaccac tcgggaccgg tatgagcgaa tccgcccatg tatccctgcc
    16741 gatgtggctg ttgtggccga aatcccctat tttctgcggg ataaacaact agtgctcttg
    16801 ggcgggaaaa aggtcttggc ccgttg
//
LOCUS       DAOSUT010000086        16580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2151_length_16580_cov_9.441755, whole genome shotgun sequence.
ACCESSION   DAOSUT010000086 DAOSUT010000000
VERSION     DAOSUT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_16580_cov_9.441755"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1335
                     /locus_tag="PLD05_09300"
     CDS             253..1335
                     /locus_tag="PLD05_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77677.1"
                     /translation="MTRLLLGTGIGLMVLFILVLMRPVISSIFQAAGPNSAKARPSRQ
                     VTRDSQDPKSSELAASIPGLNEVLGHPSSATQSSGPPTETNRESRVAHLTRLVAASQA
                     FGTTAQKVQDSHGHVEMKIEECQIAPVALRRGSVISKTARPYLAFKLRVRNTSSSETV
                     NYQSPRQGAEVAEIWWDKATQTTPPWQPRGMTLEGQAENATLQPGQELVDAFAFQVPP
                     EEFAQLYIRIPGRSVNLAEDFLFVIPKNEVQRPQDSDRAPSAGESDVESIGPMSVTKQ
                     AEPPTAHADSPQAEVSPSDEDEEAIPIPGLSNAQGPASGEGGMDQEERTKLEELRKRG
                     EALQEILDRQHENRRPGGSRPQSQRR"
     gene            1416..2696
                     /locus_tag="PLD05_09305"
     CDS             1416..2696
                     /locus_tag="PLD05_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP77678.1"
                     /translation="MNQIRKVVVFSVSFVFLFLWFSTRVMANENAVIWPRFRGPNGLG
                     VCDLYEFPAQIEPGNLAWQIELDGVGHASPVVAHGRVFIASANPETGEQILAAYATKD
                     GHLLWRHRLPGKTFPKHAFNSFASSTPTLDEKRLYYAWATPESLQLVAMQQENGEIVW
                     TKDLGPFVSQHGFGASPIVYKDTVILPNEQDGESFIVALECATGNIRWKTPRRSEKAA
                     YSTPTIFQKAGIKDQLILTSWAHGIASLDPETGTPLWELPIFHNRTVGSPLVVGDLIV
                     ASSGEGGIGREMFVVRAGIPETGRKPELHYEIRGSLPYVTTSVAYKGLLYLLFDKGIL
                     ICQRLNDGQSLWKERIAGEYFSSPLVLGGKLYCLSRSGMLVVVATGEKYRMLGQYDFG
                     EATHATPAVADGLLLVRTQSHLFAVKSIKSPPSK"
     gene            2888..3484
                     /locus_tag="PLD05_09310"
     CDS             2888..3484
                     /locus_tag="PLD05_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77679.1"
                     /translation="MLVATAIFFFCLGWLANYRPVKQSGTPGKTVEGGTPVLLSGQLR
                     YGRPTGLPIPDAGGVVIVLPVGATPERPIPIEGLRPWDSNSAVGQINRQRLTELGGTW
                     TQTAEDGSYSVVLPRPGQYWVLAISKNLARPKEVLELTRRGIAELDYEQLSRYFERPA
                     DLIGPQAYRWSLETIGPAGLQLNHDFQVHGELGIPELP"
     gene            complement(3517..4011)
                     /locus_tag="PLD05_09315"
     CDS             complement(3517..4011)
                     /locus_tag="PLD05_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOP77680.1"
                     /translation="MSDQEKIWFRQGEIHDVVIRQLKRHCDVRGWLVELFRHDEIAEA
                     YYPVMAYASETLPGIARGPHEHRDQTDFFAFIGPGDFKLYLWDARPTSPTFGVFQSLV
                     VGKSQPTSVLIPPGVVHAYKNISDEPGLVFNAPNRLYAGWGKKEPVDEIRHEDLADSP
                     FQLD"
     gene            4324..5745
                     /locus_tag="PLD05_09320"
     CDS             4324..5745
                     /locus_tag="PLD05_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOP77681.1"
                     /translation="MADKAPPRNIARIFDRIEQPIYFIDQRWHIRYCNRACLEWLKCS
                     KEEILDRRCAFHTGGNLDPGDALAAGLCPPPGAMESHESVGIVVCLQPTGELKRRRGR
                     FLRLTTTSGVLGILGILDQHDLPDSPAPDRLPKSPADSLLSLDDGTQEAFRLHEELRQ
                     SWRTVLHKGWPPLLVGSSATVRRLRQQIEALSKITLSVCIVTPPGGGGGLLARAVFHG
                     AEPHPQQCLIPLPCEDLDPVVLRMTLQVFDSVPPPPQEFPHTLLLENVDRLPNVSQEV
                     LCHILASKPGSYRVLAVSNQSLDQLVESGTFLPELARMIAHFTVLVPSLAERREDLPL
                     MAQAVLEDLNVRGPKQVLGFSREVLDLFAQYDWPRNFEELAWVIDQCYQATSQPIIDV
                     DALPRELQWSMKHKMRLLRDPETISLPTFLADVEKELLLRAMQRARGNKTRAAKMLGL
                     SRARLYRRLLQHKLIKPEDLHAE"
     gene            complement(5989..7245)
                     /locus_tag="PLD05_09325"
     CDS             complement(5989..7245)
                     /locus_tag="PLD05_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77682.1"
                     /translation="MSLARSKVTRRAFLAGSAAAVSFFGKRLPAEVEGVPIRAITKGP
                     GFHWFAYYDKFQFDPTDRYALGMKVLFEHRSPTPQDSIEIGMIDLAEGDRWIPLGETT
                     AWCWQQGCMLQWRPGSATEIVYNGRGDEGYVCHIQDVVTGAKRTISSPIYALAPDGRT
                     AVTPDFRRIANLRPGYGYVGFPDPFAHERAPEQSGIWRVNLESGRSDLIISLAQAAAI
                     RWPYADFGDAVHWFNHLLVNPTGTRFVFLHRWKLPDEKSWRTRMFSSNMDGSELRVLI
                     GSGFVSHFIWRDETHVLAYSKPEPDAAWGFYLYEDVPNGRVEQVGAGVMGPGDGHCNY
                     LPGNEWIVCDTYPDKNRYQHVYLYHVPSNRRIELASLLSPAEYAGEWRCDTHPRVSRT
                     GKFITVDSPHEGGREIYLIDVSKIIG"
     gene            7553..10459
                     /locus_tag="PLD05_09330"
     CDS             7553..10459
                     /locus_tag="PLD05_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOP77683.1"
                     /translation="MPRERRNFLGTLSRILVTVAIARPLVTIGIGLLTAAVAIYCGIN
                     FIQIHTSRADLLNSKNEYHRRWLAYVNEFSDKEDVYVVVEGDNPNAVAPALIELAEAL
                     RREPQYFTSVLERVDKTPLKRKGLYYLSTQELQQIVSHVRQLKNTLTQLAPVLRPANV
                     CLEAAAMISQIPPGYPSHQAFGQIAQVIQYLAVVLNSPPEQLAETILTQIAANNQQGD
                     FAFGQGLGVLLDPEKTTDLREEMFFSHDGHLGLVILRFTPDRSGQFTRFAAHIRRLRE
                     ITSDVNRRYPNLRIGSTGLPILEYDEMRASSSSAMVAVLSLAGVILVFFAGFGGIRYP
                     VAVTFSLVTGVCWAVGFVVIAVGHLNILTSAFGAMLIGLGDYGVHFVAHYLDRKKRQP
                     NVRDTLIETAETVGPGIATGAVTTAVAFFAGAFTDFLGVAELGIIAGGGILLCWLADM
                     TILPAFLCLIDRDGLRSQKSALLDLTPWFRPIAHFPKAVTLGVGAVVTLLAMGLPHLR
                     YDYNLLHLQPKGLESVEYQNKLVEHMSRSSYFALSIAANPEEAKRRKAEFLKLPTVDH
                     VEDLVSVLPDDVAVKRTLITQLAAELKDWSCLDTNIPSREVLDQGLAGLQMALQHQPH
                     AESAQLQMIVNHLRTTIRSLPAEEYTRRLSYLTQTIQREILPAFASLALLTQQLDPPQ
                     LDDLPQPLVERFVGKNGRHLLQVYCKGDFWDPVTMRQFVADIRSVDPAATGNPIQIYE
                     ACHQMNRAYIEAAIYAFVAILLTLYLDFRSIKDVLLSLLPLILGMISLFGLMGWFDLP
                     LNPANMITLPLILGIGIDDGVHILHDFRRQGNRYRMPEGATLTAVTINSLTTLVGFGA
                     LMASPHCGLESLGRVLTFGMAFCLVTALLMPGFLRWASGLLRGNRDSTEWETELQHIL
                     KGVTAGQHPTSEPHQAATNPPRVVAPLSAAEEQPQSEQSQRAAISVKIRPRRAA"
     gene            complement(10489..>10824)
                     /locus_tag="PLD05_09335"
     CDS             complement(10489..>10824)
                     /locus_tag="PLD05_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77684.1"
                     /translation="MMRLAILGIITGVMIFCVVGLATVLTLAAVVSGMGDVTAGIVLR
                     YVALGIGVFLAVDTVVLLLWLVFLTVTAELALNQVIAAKKDQDKQMDSTTVAMENLAD
                     SEWEETSSG"
     assembly_gap    10825..10924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10925..12886
                     /locus_tag="PLD05_09340"
     CDS             <10925..12886
                     /locus_tag="PLD05_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOP77685.1"
                     /translation="LDVIQGITELTPQFTRVQGLSHVWDMRLATTSLPKETLYKILLQ
                     AAGGTPDVETRKRIARFLIQMGRYEDALRELEDLLAEPQLPEDQKNDLQQSVRALKQL
                     SARRLVEELRMRQKAGQYETVYALLQKFPTEGIAGEILQEVKELLADYEKKYTTAQDL
                     LSTLDALIQLIDETVTRQLCSEILKEIAADLTPDTLPRLAAFAQLAHDESLPPEERVA
                     LAISGWFLGADAAFRRLPTALSLYRVRNLMVEYLNEEQVLKRQEILERLRREEASAPR
                     YAAGILAHMRPPKPLPEEPPEKYGFLEMEITWDSKEPPARYLVQLPPEYHPLRKYPTI
                     VTLHGLQTTPEQQIDWWAGEFDDKGRRIGQAGRNGYIVIAPQWTQEQQVAYQYSAREH
                     GLVLAVLRDAIQRFSIDTDRVFLTGHFSGGDAAWDIGLAHPSLWAGVIVISGRSDKYC
                     TYYWENAAHVPFYIVQGELDGTSMPDNARDLDRYLNRAFDVTVVEYMGRGRDPFFDEI
                     LRLFEWMSYKQRNPAPTDFTARSMRAFDNFFWFIELADMPPAVITPPVNWPPRNPTPL
                     VVRGRIPSPNTLLLQVGSARSIIGLGPEFVDFDKRLAVTINAQRVDTRDVQPDIEVLL
                     EDVRRRGDRQHPFWAKIETATGRVSGKRP"
     gene            complement(12950..14338)
                     /locus_tag="PLD05_09345"
     CDS             complement(12950..14338)
                     /locus_tag="PLD05_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOP77686.1"
                     /translation="MFLSPMLVLLIGVALVILLIVVVRVNAFLALIAAAFVVSLLSPG
                     AWDEKIARAVTEFGATAGKIGVMIALAAVIGKCLMDSGAAERIVRTCLRLIGESRAPV
                     ALMTSGFVLSIPVFFDTVFYLLVPLARSLSRMTKSHYSLSILAIGTGAALTHTLVPPT
                     PGPLAVAGTLGVEVGHMILIGILVGLPAAVLVYPIIRLLDRSMPLPVRPYVGEVETPP
                     AQEQDLPPFWLSAMPVILPVLLISANTIIKVLVKSYPSWQTLANVTGVIGDPNAALLL
                     AAVVAMGTVAWKRHLSLEALGRLTEEALMSAGVIILITSAGGAFGAMLRQTGINEEIK
                     VFVGSQSSMGGVVLLAMSFLVAAVMKTAQGSSTVAMLTTAAIMTALFDTPESLVQHLG
                     CHPVYMALAIGAGSLVFCWMNDSGFWVVARMSVWSELEALKLWTVTAACAGLAAFLVT
                     VVFALVLPNALG"
     gene            complement(14504..16384)
                     /locus_tag="PLD05_09350"
     CDS             complement(14504..16384)
                     /locus_tag="PLD05_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77687.1"
                     /translation="MGSKTRAFCEVLFGVVAWSVMNVLWALPPAEKLFPDTARGFLAV
                     RDVSELREHFDQTQIGQLVKDPALRPFIEDIRLQIRRRLVGAQQELGISLEDVAQLAS
                     GEVAVGLVFAENAERPVLAALACIKGKEKESQELFDRIGEEMKKQKATVTKTSVAGKE
                     VWAIKLAEDAESGRSREFFHYRDDEVILATSNKDVLRFMLEQLQAPGKGSLADVPAYQ
                     EIMKRCQQDAGENQPHIRWFIEPFEYTAAIRATIPPEQRRRQRQLAEALKKAGFSVLQ
                     GIGGTVSFASEGFELVHRTFVYAPGPFEKSAKILSFPNNTDFTPPSFIPRDLATLSIG
                     YCNILQGFDSIGPLFDETVGEGEEGVWEDVLASLKEDPNGPQIDLRNEFFAHFGEKVS
                     IVSAYQLPITTTSERMLIAINVKDEKAAAQGLDKMFQYEERFRKREYKGHTIWEGLPE
                     EKPKVPKVELGGIPGVKGEEVAEEASEPLFPNASLTVDRGYLLIASHYDYLTQILDLE
                     DERESIKRNPAFLEVMEAIEKAGGKTACWRFFSMTDEEYRASYELIRQGKMPESETML
                     GRLLNAILAPPEKGVTRKQRIDGSKLPDYEIVRRHLGPAGFFAVSEDQGWFLKGFTLP
                     KK"
BASE COUNT         3807 a         4737 c         4251 g         3685 t
ORIGIN      
        1 ggtcgtctcc agccgtcaga cgctgtagct caaacggcaa atggaccgtg gtatcttgcg
       61 tctcacgtcc gcgggctgtt cccgcaatca gaagtagtgc catcccgaac tccggcgagt
      121 ccgccaacga ctcctgttcc cacccctccg gtaagtgtag ggccgccttc atccactcag
      181 acttcgggga atgtagtaac ccccgaacag ctcgcaaaat ggcgggcaca acgtcggcaa
      241 cgcgctcgga agttgacaag gctgttactg gggacgggta tcggcctgat ggtgcttttt
      301 attctcgtcc tgatgcggcc tgtcatctcc tcaatatttc aagcagcagg tccgaacagc
      361 gcgaaagccc gcccatcccg ccaggtgacc cgcgattcgc aagatccaaa atcgtcagag
      421 ctggctgcaa gtattccagg acttaatgag gtcttgggac atccatcgtc ggcaacgcaa
      481 tcttccggcc cacctacaga aaccaatcgg gagtcgcggg tagcgcatct gaccaggctt
      541 gtcgctgcca gtcaggcctt tgggacaacg gcccaaaagg tccaggactc tcatggtcac
      601 gtggagatga aaattgagga atgccaaatt gcgcccgtgg ccttgcgccg gggaagcgtc
      661 atttccaaga cggcgagacc ttacttggcg ttcaagctgc gggttcgcaa tacctcgtcg
      721 tctgagacgg tgaattacca aagtcctcga caaggagctg aggtggcaga aatctggtgg
      781 gacaaagcca ctcagacgac tccaccttgg cagcccaggg gaatgactct tgaaggccaa
      841 gcggagaacg cgacgcttca gcctggccag gagttggtgg atgcctttgc ttttcaggtt
      901 cctcccgagg aatttgcaca actctacatc cgtatccccg gacgttccgt aaatctggcg
      961 gaggactttt tgtttgtgat accgaaaaac gaggtgcaac ggcctcaaga ttcagatcgg
     1021 gccccctcgg caggagagtc cgatgtagag tcaatcgggc cgatgagtgt gacaaaacaa
     1081 gctgagcctc caaccgctca tgcggattcg cctcaagcgg aggtgagccc gtcagatgag
     1141 gatgaggagg caatccccat tccgggccta agcaatgccc agggaccggc ttccggggag
     1201 ggaggaatgg accaagaaga gcgaaccaaa ctcgaagaac tacgtaagcg cggcgaagcg
     1261 ctccaggaga ttttggatcg ccaacacgaa aaccgtaggc ctggtggatc acgtcctcaa
     1321 tcgcaacgac gttgatcgaa aaagtaggaa gagacatttc atctcaagag gagataatct
     1381 tcataggaca cgacgctcca aaggaacggc cgcccatgaa tcagattcgc aaagttgtgg
     1441 tcttctccgt atcgtttgtt ttcctattct tgtggttttc cacgcgggta atggccaacg
     1501 aaaacgcggt catttggcca cggtttcggg gcccaaatgg gttgggcgta tgcgatctgt
     1561 atgagttccc cgctcagatc gagccgggca acctcgcgtg gcaaatcgag cttgacgggg
     1621 tagggcacgc ctcgcctgtt gttgcgcatg gtcgagtgtt tatcgcctcg gcaaatcccg
     1681 agacgggcga acagattctt gctgcctatg cgacaaagga cgggcactta ctttggcggc
     1741 accgcctacc cggcaagaca ttccccaagc atgcgttcaa tagtttcgct tcatctacgc
     1801 ccactctgga tgaaaagcgg ctttattacg cctgggccac tccggaaagc ctccagctcg
     1861 ttgcgatgca acaggaaaat ggggaaattg tttggacaaa agacctcggc ccatttgtgt
     1921 cgcaacatgg ctttggggca tcacccattg tttacaagga tacggtcatc ctgcctaacg
     1981 aacaggatgg cgagagtttt atcgtggctc ttgaatgtgc cacaggtaat atccgttgga
     2041 aaacgccgcg gagaagcgaa aaggccgcat attcgacccc aacaatcttc cagaaggccg
     2101 gcatcaagga tcagcttatc ctcaccagtt gggcgcacgg cattgcgagt ctggatcccg
     2161 aaacgggaac cccactgtgg gaactgccca ttttccacaa tcgaactgtt ggttcaccac
     2221 tcgtagtggg tgacctcatc gtagcgagtt ccggggaggg aggaatcgga cgagaaatgt
     2281 tcgtcgtccg tgccgggatt cccgaaaccg gccgcaagcc agagcttcac tatgagattc
     2341 gtggttcgct tccgtacgta acaacatctg ttgcctataa agggttgttg taccttttgt
     2401 ttgacaaagg catcctcatt tgccagcgtc tcaacgacgg ccagagcctt tggaaggaac
     2461 gaatcgcggg ggagtacttc agttcgcctc tcgtcctcgg aggaaagtta tattgtcttt
     2521 cccgatccgg catgctcgtg gtcgtggcaa cgggggaaaa gtaccgaatg ctgggacaat
     2581 acgattttgg tgaagccacg catgcaacac cggccgttgc ggatggatta ctgttggtgc
     2641 gaacgcaaag ccaccttttt gccgttaaat cgatcaaatc accaccgtca aagtgaactc
     2701 aggacagaac gaaaccaaac ttgcggagcc atcggggatc agccccacgc taagggagag
     2761 ccctccggtt gccccgccct tgcgccagcc tggccatggg gcttcgtcgg cgccaacaga
     2821 gaggcgccaa ttatccgctg ccgatgtgat cgttgtgccg agaaaccgca tacggtggta
     2881 ctatttgatg ttggtcgcga cggccatttt ctttttttgt cttggctggc tggccaacta
     2941 tcggccggtt aagcaatcgg gtacaccggg taaaactgtc gaaggaggta cgcctgtgct
     3001 tctttccggc caactccgtt atggccgccc gaccggatta ccgatccccg acgcgggagg
     3061 cgttgttatc gttcttcctg tgggtgcaac tcccgaacgt cccatcccta tcgaaggtct
     3121 tcgcccctgg gatagcaact ccgcggtggg ccaaataaac cgtcaaaggc ttaccgagct
     3181 cggcggtacg tggacccaaa cggccgagga tggaagctac agtgttgtgt taccccggcc
     3241 gggtcaatat tgggtcctag caatctcgaa gaacctagcg cgcccgaagg aagtcctcga
     3301 gctgacacgg cgtggcattg ctgaactcga ttacgaacaa ctttcccggt actttgaacg
     3361 accggccgat ttgatcggcc ctcaggcata ccgctggtca ctcgaaacga ttggtcccgc
     3421 cggactgcaa ctgaatcacg acttccaggt tcatggggaa ctggggatcc cagaactgcc
     3481 atgacaaaag gttggccttc tctgcccgac gccggatcaa tccaattgaa atggcgaatc
     3541 ggccaagtcc tcgtgacgga tttcatccac aggctctttt ttgccccagc cggcataaag
     3601 acggttcggt gcgttgaaga ctaatcccgg ttcgtcggag atgtttttgt acgcatggac
     3661 gactcccggc gggatgagaa ctgatgtggg ctgtgacttt cccacaacaa gggactgaaa
     3721 aaccccgaag gtgggtgacg ttggacgtgc atcccagaga tagagcttga aatcccccgg
     3781 tccaataaac gcgaagaaat cggtttggtc tctgtgttca tgtggcccgc gggcaattcc
     3841 cggtaaggtc tcactcgcgt aggccatcac cgggtaatat gcttcggcga tctcatcatg
     3901 ccgaaaaagt tcgaccaacc atccccggac atcacaatgc cgcttgagct gccgaatgac
     3961 cacgtcatgg atttcacctt ggcgaaacca tattttttcc tggtcgctca tgcgatttac
     4021 ccgtcttgac tcttcctgac cttatgaaaa actatttccc tttgattcga ggccaaacgt
     4081 cacagagcac aattcctttc gctattttac tctgattttt tccgacggtc ttgcggtcga
     4141 acggacttga cactatggca gcagcgcact catcttcttt tccactcacc ggcaagagtt
     4201 cattcaaacg tgacagactt ttctggcaaa gcgataatac gcttaggacg tgccgtgatg
     4261 acactgttac cgtatcttgt aagagatgtg gtgtgaatcc cgtgtgcgga aggaagtgag
     4321 ttgatggctg acaaggcacc gccccgaaat atcgcgcgca ttttcgatcg catcgaacag
     4381 cccatctatt ttatcgacca gcgctggcac attcgttatt gcaatcgggc ttgtcttgag
     4441 tggctgaagt gcagcaagga agagatactc gatcgccgct gcgcgtttca tacgggtggc
     4501 aaccttgatc caggcgatgc tttggccgcc gggttgtgcc ctccgcccgg ggcaatggag
     4561 tcccatgaga gtgtcggcat cgtcgtgtgt cttcagccca caggagaatt gaaaagacgt
     4621 cggggaagat tcctgagact caccacaacg tccggtgtct tgggcattct cggtatactc
     4681 gaccagcatg atttgccaga ttcacctgca ccggatcgat tgccaaaaag tccagcggac
     4741 tcattactat cgctagacga cggaactcag gaggcatttc ggctacatga ggaattacgg
     4801 caaagttgga ggacagtcct gcataaaggt tggccgcccc tccttgttgg ttcaagcgca
     4861 accgttcgtc ggttgcgtca acagatcgaa gctctgtcta agattacttt aagcgtttgt
     4921 attgtcacgc cgccgggtgg tggtgggggc cttttggcca gggcagtttt ccatggagcg
     4981 gaacctcacc cgcagcagtg tttgattccg cttccgtgcg aagacctcga ccctgtcgtc
     5041 ttgaggatga cgcttcaagt tttcgattca gtaccacctc cgccacaaga gtttcctcac
     5101 actttgctgc tggaaaacgt tgaccgactt ccgaacgttt cccaggaggt tttgtgtcac
     5161 attctggcct caaagccagg gagctatcgg gtattagccg tcagcaacca gtcgcttgat
     5221 caattggttg aatccggaac ctttttgccc gaactcgccc ggatgattgc acacttcacc
     5281 gttttagtcc ctagcttggc tgaaaggcga gaggaccttc cgctcatggc ccaggcggtc
     5341 ctagaagacc tcaatgtcag ggggcccaag caggttttgg gtttttccag ggaagtcttg
     5401 gatttgttcg ctcaatacga ctggccgcgg aactttgagg aacttgcatg ggtgatcgac
     5461 cagtgctacc aagcaacctc tcaaccgatc attgatgttg acgccttgcc acgcgagctg
     5521 caatggtcca tgaaacacaa aatgcgactt ctccgcgatc cggaaacgat ctccttgcct
     5581 acgtttcttg ccgatgtgga aaaagagctc ttgcttcgcg ctatgcagcg tgcacggggc
     5641 aacaagacac gggctgccaa aatgctgggg ctttcccgtg ccaggctcta tcgccggctc
     5701 cttcaacaca agctcattaa gccggaagat ctgcacgctg agtaaatacc tgcagaccga
     5761 ttccggagcc tcccacaagt aaaccaccgg aaacacgtag caggtcgtaa aaggtcattt
     5821 agagtattgc ataatctttc cctcacccgt tcgacgggag aggggtcggg gctgagggtg
     5881 gcgcacatcg ctgccggttt tctcgatcga ctccgacgac cgctcgccca caccctaacc
     5941 ctctcccaga gggagagggg gccttttagg gatatatcgg ggagtcagtt aaccgatgat
     6001 ctttgacaca tcgatcagat aaatctctcg cccaccctca tgcggtgaat cgaccgtgat
     6061 aaacttgcct gtccgactta cccgcgggtg agtgtcacat cgccattctc ccgcatactc
     6121 cgcgggcgac aacaacgacg ccaactcgat ccggcgattt gaaggtacgt ggtaaagata
     6181 gacatgctga tatcggttct tgtcgggata ggtgtcgcat acgatccact cgtttcccgg
     6241 cagatagttg caatgtccat cacctggccc cataaccccg gcgccgacct gctcaacccg
     6301 gccattggga acatcttcgt acaagtaaaa tccccacgcg gcatccggct cgggtttcga
     6361 ataagcaagc acatgggttt catcccgcca aataaagtgc gatacaaatc ccgagccaat
     6421 cagaaccctc aactcgcttc cgtccatgtt tgacgaaaac attcgtgtcc gccagctctt
     6481 ttcgtcggga agtttccagc gatgcagaaa gacaaatcgc gtgcccgtgg gattaaccag
     6541 gagatggtta aaccaatgga ccgcatcacc aaagtcggca tagggccacc ggatagctgc
     6601 ggcctgggcc aacgagatga tgaggtccga tctcccagat tccaaattta cccgccaaat
     6661 ccccgattgc tccggtgccc gctcgtgcgc aaagggatcg ggaaacccca cataaccgta
     6721 acctggccga aggttcgcga tccgacgaaa gtcgggcgtc accgccgtgc gcccgtccgg
     6781 ggcaagagcg taaatgggtg aagaaatcgt cctcttagcg ccggtaacaa catcttgaat
     6841 gtgacacaca tagccctcgt caccccggcc attatagacg atctcggtgg ccgagcccgg
     6901 gcgccattgc aacatgcaac cttgctgcca acaccacgcc gtggtctcgc ctagaggaat
     6961 ccagcggtca ccctcggcca ggtcaatcat gccaatttca atgctgtcct ggggcgttgg
     7021 tgatctgtgc tcgaatagca ctttcatacc aagtgcatat cggtccgttg gatcgaactg
     7081 aaacttgtca taataagcaa accaatggaa tcccgggccc ttggttatcg ctcggattgg
     7141 cactccctca acctctgccg gaagtctctt cccaaaaaag gaaaccgcgg cggctgagcc
     7201 cgccagaaaa gcccgccgcg tcaccttact tcttgcaagg gacataggta attcccctca
     7261 tttaaatcga gaaagttagt aaacctttgt gttaacgcgc gattctggct ggttatccta
     7321 accgaagccg ttgccagatc aaagaggcag gccgagggtc tcgtctcaaa cacatgcctt
     7381 tttgctttga caccgtgaac gtggcctctc ctgtgaccga atgtcttctg gcctattctt
     7441 tgcgccagtt ggcttcctta tcactcttgt cgcaaaaagg acctttatgc tactataacg
     7501 ccctggttgt gacaaatctt tgtgtggtct aacattcttt caggggtcac ggatgccccg
     7561 cgaacgacgt aatttccttg gaacgctttc gcgaatctta gtgaccgtgg cgatagcacg
     7621 ccctctggtc acgatcggaa tcggtcttct gacggctgcg gtcgcgatct attgcggaat
     7681 caattttatc cagatccaca ccagtcgggc cgacctcctc aattccaaga acgagtatca
     7741 ccggcgttgg ctggcctatg tgaacgagtt ttctgataaa gaggatgttt acgtcgttgt
     7801 agagggggat aatccaaatg cagttgcccc ggcccttata gaacttgccg aggcccttag
     7861 gcgagaaccg cagtacttca catcagtgtt ggagcgtgtc gataagacac ctctcaaaag
     7921 aaaggggctt tattacctct ccacgcagga actccagcag atcgtaagcc acgtccgcca
     7981 gttaaagaac acattgacac aactggcccc tgtcctccgg ccggccaatg tgtgcctgga
     8041 agcggccgca atgatttcgc aaatccctcc tggttatcct tcgcaccagg cgtttgggca
     8101 aatcgcccag gttattcaat acctcgccgt tgttctgaac agccccccgg aacaactggc
     8161 cgagacaatt ctcacgcaaa ttgcagcgaa caatcaacaa ggcgacttcg cttttggaca
     8221 aggactcggc gtcctcctcg atccagaaaa gacaacggac ctgcgtgagg aaatgttctt
     8281 ttctcatgac ggccacctgg gtcttgtgat cttgcggttc acccctgacc gaagcggaca
     8341 atttacgcgg ttcgccgctc atatccggcg tctgagggaa atcacaagcg atgtaaaccg
     8401 gcgctacccg aaccttcgga ttggctccac aggcctaccc atcctagagt acgacgagat
     8461 gcgggcaagt agctcttccg ccatggtcgc cgttctctcg cttgccggtg ttatcctcgt
     8521 gttttttgca ggcttcggtg gaattcgcta tcctgttgct gtcacatttt ccttggtgac
     8581 cggtgtgtgt tgggccgtgg gatttgtcgt aatcgccgtg ggacatctca atatcctaac
     8641 gagtgccttc ggggcgatgc tcatcggcct tggtgattac ggcgtccatt ttgttgctca
     8701 ttacctcgac agaaaaaaac ggcaaccgaa cgtccgagat accttgatcg aaacggcgga
     8761 aaccgttggg ccaggcatcg ccacaggcgc cgtgaccacg gcagtcgctt tttttgcggg
     8821 tgccttcacc gatttcttgg gtgtcgccga gctagggatt atcgcgggtg gaggaatcct
     8881 cctttgctgg ctagccgata tgacgatact tccggccttt ctctgtctga tcgatcgcga
     8941 cggactgcgt tcccaaaagt ctgcccttct tgatctaaca ccgtggtttc gccctatcgc
     9001 ccacttcccc aaggcggtca ccttgggtgt tggagcggtc gtcaccctat tggcaatggg
     9061 gttacctcat ctccgttatg actacaacct cttacacctc cagcccaagg gactcgaaag
     9121 cgtggagtac caaaacaagc tcgtcgagca tatgagccga agtagttatt ttgccctttc
     9181 tattgctgct aaccccgaag aggccaaacg ccgcaaggcc gagtttctca agctccccac
     9241 cgttgatcac gtggaagatc ttgtttccgt ccttcccgat gatgtcgcgg tgaaacgcac
     9301 actcatcaca caactggctg ctgaactcaa ggactggtcc tgtttggaca ccaacatccc
     9361 ttcgcgagaa gtcctcgatc aaggcctagc ggggttgcaa atggccttgc aacatcaacc
     9421 ccatgcggag agtgcccagc ttcagatgat agtcaatcac cttcgcacca ccatccggag
     9481 tttgccggcg gaggaataca cacgccgcct ttcgtatttg acgcagacaa ttcagaggga
     9541 aatacttccc gctttcgcca gcctcgctct tctcacccaa cagttggatc caccccaact
     9601 tgatgacctg cctcaaccgc ttgtggaacg ttttgtcgga aaaaatggcc gacatcttct
     9661 gcaagtgtac tgtaagggtg atttttggga tccagtgacg atgcgacagt tcgtcgcgga
     9721 catacgcagc gttgatcccg cggcaacggg aaatcctatc cagatttatg aggcctgcca
     9781 tcagatgaat cgagcctaca tcgaagctgc gatttacgct ttcgtcgcca tccttcttac
     9841 cttgtatctt gattttcgca gcatcaagga tgttctcctg tccttgcttc ctcttatctt
     9901 gggaatgatc agcctttttg gactaatggg ttggttcgac ctgccactta accccgccaa
     9961 tatgattaca ttgcccctta tactagggat cggcatcgac gatggggtgc acatcctgca
    10021 cgattttcgc cgccaaggaa atcgctatcg catgccggaa ggggccacgc tgaccgcagt
    10081 gacgatcaac agtctaacca ccctcgttgg ttttggggcc ctcatggcct cgcctcattg
    10141 tggactggaa agccttgggc gggtcctgac gtttggcatg gccttttgcc ttgtcactgc
    10201 cctacttatg cccggatttc tcagatgggc atccggcttg cttcgaggaa atcgcgattc
    10261 caccgaatgg gaaacagagc tgcaacacat cctcaaagga gttacggccg gacaacatcc
    10321 aacttccgag ccacatcaag cggctactaa cccaccacga gtagtggctc cattatctgc
    10381 tgctgaggag caaccccaat ccgagcagtc ccaacgcgcc gcgatctctg ttaaaatccg
    10441 tccaagacgg gcagcttaag acgcggagtc attcggtgat tccaatcatc agccggacga
    10501 ggtctcttcc cattccgagt ctgccaaatt ctccattgcg acggtcgtcg aatccatttg
    10561 tttatcctgg tcttttttcg cggcgattac ctgatttaaa gccaattctg ccgtgaccgt
    10621 caaaaacacc agccacaaga gaagaaccac cgtatccacc gccaggaaga ccccgattcc
    10681 caaagccacg tatcgtagca ctatgccggc tgtgacgtca cccattcccg atacaacagc
    10741 ggccagtgtt aggaccgtcg ccaatcctac aacgcagaaa atcatcaccc cggtgataat
    10801 ccctaaaatt gctaatctca tcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnncttgac gtgattcagg gaatcaccga actgacgccg caattcacgc gagtccaagg
    10981 cctttctcat gtgtgggata tgcgtctagc cacgacgagc cttcccaaag aaactctgta
    11041 taagattctt ctccaagcgg ccggtgggac gccggatgtt gaaacgcgga agcggattgc
    11101 ccgattcttg attcagatgg gacgttatga agacgccttg cgggaattag aagaccttct
    11161 cgccgaacca cagcttccag aggatcaaaa gaacgacctt cagcagtcag tacgagcact
    11221 caaacagctt tccgcgcggc gcctggtgga ggaacttcgc atgcggcaga aggcgggtca
    11281 gtatgagacc gtctatgcgc tgttgcaaaa gttcccaacg gaaggtattg ccggagagat
    11341 cttgcaggag gtcaaagagt tactggcgga ttacgaaaag aagtacacca cggctcagga
    11401 tctgctatca actctggacg cgctgatcca actgatcgac gagacagtaa cccggcaatt
    11461 atgttccgag atcctcaagg agattgccgc ggatcttaca ccagacacct taccccgact
    11521 tgcggcattc gcccaattgg ctcacgatga gtcacttccc ccagaagagc gggtggcgct
    11581 ggcaatcagc ggctggttcc ttggggccga tgccgctttt cggcgtttgc ctacggcact
    11641 ctcgttatat cgtgtgcgga atctgatggt ggagtatttg aacgaagagc aggtgcttaa
    11701 gcgtcaggag atcttggaaa ggctacgacg tgaggaagcc tctgcacccc gttacgcggc
    11761 aggaatcctt gcccacatgc gacccccgaa gccgctccct gaggaaccgc ccgagaaata
    11821 tgggttcctt gaaatggaga tcacctggga tagcaaagaa ccgccggcgc ggtatcttgt
    11881 tcaattgccg ccagagtatc acccgctgcg caagtatcca acgatcgtca cgcttcatgg
    11941 cctgcaaacc accccggaac aacagatcga ttggtgggcc ggggagttcg acgacaaggg
    12001 gcgtcggatc ggtcaggccg ggcgaaacgg ctacatcgtc attgcgccac aatggactca
    12061 agagcagcaa gttgcctatc agtactctgc ccgggagcat ggtctggtcc tcgcagtcct
    12121 ccgtgatgcg attcaacgat tctcgatcga cacagatcgc gtgttcctga cgggacactt
    12181 ttctggtggg gatgccgcct gggacatcgg cctcgcccat cccagccttt gggcgggcgt
    12241 gatcgtcatc tccggacgat ctgacaaata ttgcacgtac tactgggaaa atgcggccca
    12301 tgttcccttt tacattgtgc agggcgagtt ggacggaaca tcgatgcccg acaatgcccg
    12361 cgatctcgat cgttatctca atcgagcctt tgatgtcacc gtcgtggaat atatgggccg
    12421 aggtcgcgat cctttcttcg acgagattct tcgtctcttc gagtggatgt cttacaagca
    12481 aaggaatccg gctccgacgg attttacggc tcgttcgatg agggcgttcg acaatttctt
    12541 ctggtttatc gagcttgccg atatgccgcc ggctgtcatc accccgccgg taaactggcc
    12601 gccacggaat ccgacaccgc ttgttgtccg cgggcgaatc ccctcgccaa acaccctctt
    12661 actccaagtg ggctcagccc gaagcatcat tggcttgggg ccggagtttg tcgatttcga
    12721 caagcgtctc gctgtcacga ttaatgctca acgagtggac acgcgggacg ttcagccaga
    12781 cattgaggtc ctgctggaag acgtgcgtcg ccgcggcgac cggcaacatc ctttttgggc
    12841 gaagattgag actgcgacag gccgtgtctc gggcaaacgg ccatgagcta tccggccgtc
    12901 gaaacgagat cacaattaac cgactttttt gacgtggttt gcgcgaacat cagcccaatg
    12961 cattggggag caccaaggca aagaccaccg ttaccaaaaa tgcagccaaa ccagcgcagg
    13021 ctgccgtaac cgtccacaat ttgagcgcct caagctccga ccaaacgctc atccgagcaa
    13081 cgacccaaaa accactatcg ttcatccaac aaaagacgag cgaccctgcg ccgatggcca
    13141 atgccatata gaccgggtgg catcccaaat gctgaaccaa cgattctggt gtgtcgaata
    13201 gtgccgtcat gatggctgcg gttgtcaaca tggccacagt gcttgatccc tgagccgtct
    13261 tcatcactgc cgccaccagg aaactcatcg ccagcaaaac gacacccccc atgctcgatt
    13321 gcgatcccac aaacactttg atttcctcat tgatgccagt ttgccttagc atggccccga
    13381 aagcgccacc tgccgaagtg atgagaataa tgactccggc gctcatcaat gcctcttcgg
    13441 ttaatcgtcc caatgcttcc aacgacaaat ggcgtttcca cgctaccgtc cccatagcaa
    13501 ccacggccgc caaaagaagt gccgcattcg ggtccccgat cacgcccgtg acattcgcaa
    13561 gagtctgcca actcggataa gatttaacca gcaccttgat gatcgtgttg gcggatatca
    13621 gcaaaaccgg aaggatcaca ggcatcgcag atagccaaaa cggcggcagg tcctgctcct
    13681 gcgctggtgg cgtttccacc tcgcccacat agggccggac gggaagcggc atagaccgat
    13741 cgagaaggcg aataatggga tacaccaaaa ccgcggcagg cagtccgact aaaatcccga
    13801 tcagaatcat gtggcccacc tccacaccca acgtcccggc aacggccagg ggacccggcg
    13861 tcggaggaac caacgtatgc gtcagggcgg cccccgtgcc gatggcgaga atcgacagcg
    13921 aatagtgtga tttcgtcatc cgagaaagcg atcgggccaa gggaaccaac aaatagaaga
    13981 cagtatcgaa gaaaacggga atcgagagga caaaaccact ggtcatcaat gccacaggtg
    14041 cccgcgattc accaataagc cgcaggcatg tccggacgat ccgctccgcg gcaccgctat
    14101 ccatgaggca cttgccgatc acggccgcaa gagcaatcat gacgccgatc ttgcccgccg
    14161 tcgccccaaa ctccgtgaca gcccgagcga tcttctcatc ccacgcacca ggcgaaagaa
    14221 gactgaccac aaatgctgcc gcgataagtg ccagaaaggc attgactcga acaacaacga
    14281 taagcaggat gacaagcgcc accccaatga gcaacaccaa catcgggcta agaaacatag
    14341 ttgcctgagc ctaacttgga tggtccagca ataatcatgt gacagtccag gcacctggcg
    14401 accacccggc acaccgtgct tgaacgcagg gagggtcact aggcggcaca ccgaacaacg
    14461 ggggctcttt cgccctcgac atttggtatg ccgcccataa tactcatttt ttgggcagtg
    14521 tgaaaccctt taagaaccaa ccctgatctt cgctcacggc aaagaacccg gcagggccaa
    14581 gatggcggcg cacaatctcg tagtcaggca acttgctgcc atcgatgcgt tgctttcggg
    14641 ttactccttt ttcaggcggg gccaagatcg cattcagcag tcgtccgagc atggtttcgc
    14701 tttccggcat cttaccctgg cgaatcaatt cgtaactcgc ccggtattcc tcatccgtca
    14761 tggagaagaa ccgccaacat gctgtcttgc cacctgcctt ttcgatggcc tccataacct
    14821 ccaggaaggc cgggttccgt ttgatcgatt ctcgctcgtc ttcgagatca aggatttggg
    14881 tcaggtagtc ataatgagag gcaatgagaa gataacctcg atcgacagtc aatgaagcat
    14941 ttgggaacaa cggttcggaa gcctcctcgg ccacttcttc ccccttaacg cccggaatac
    15001 cgccgagctc caccttcgga accttcggtt tttcctcggg caacccttcc cagatggtat
    15061 gccctttata ctcccgtttt cggaaccgct cttcgtattg gaacatcttg tccagtccct
    15121 gtgcggcggc cttttcgtcc ttgacattga tggcgatcag catgcgctca cttgtggtgg
    15181 tgataggcag ttgataagcc gacacaatac tgaccttctc cccgaagtgg gcgaaaaact
    15241 catttctcaa gtcaatctgc ggaccattgg gatcctcttt caggctcgcc agcacatcct
    15301 cccagacgcc ctcttcgcct tcgccgaccg tctcatcaaa cagcggcccg atgctgtcga
    15361 aaccctgtag aatattgcaa taaccgatgc ttagagttgc caaatcccgc ggaataaaac
    15421 ttggcggtgt aaaatcggtg ttattcggaa aacttagaat ctttgcggat ttctcgaagg
    15481 gaccgggggc atagacaaat gtccgatgga ccagttcaaa gccctccgag gcgaagctta
    15541 ccgtgccacc aatcccctgc aagacggaaa aaccagcctt ctttagagct tctgccaatt
    15601 ggcgttgacg acgtcgctgt tcagggggaa tcgtagcccg aatcgcagca gtgtattcaa
    15661 agggttcaat gaaccagcgg atatgcggtt gattctcccc cgcgtcttgc tggcaacgct
    15721 tcataatctc ctggtatgca ggaacgtcag ccagcgagcc ttttccaggg gcctgaagtt
    15781 gctccaacat gaagcgcaaa acatctttgt tactcgtagc gaggatcacc tcatcatcac
    15841 gataatggaa gaattctcgc gaacggccag attcggcatc ttccgcgagc ttgatagccc
    15901 aaacctcctt cccggccaca gaagtcttgg taacggtggc cttttgcttt ttcatctcct
    15961 cgccgatgcg atcgaacaat tcttgagatt ccttctcctt ccccttgata caagccaggg
    16021 cagcgagcac tggccgttcg gcattttccg caaagaccag gcccacagcc acttcgccgc
    16081 tggccagttg tgccacatct tcgagggaaa ttcccagctc ctgttgcgcg cccaccaaac
    16141 gccgtcgaat ctgaagccga atatcctcga tgaacgggcg aagggctggg tccttgacca
    16201 attggccaat ttgggtctgg tcgaaatgct ctcggagctc cgagacgtcg cgcacggcta
    16261 agaaaccgcg cgccgtgtcg ggaaagagct tttccgccgg gggcaacgcc caaaggacgt
    16321 tcatcacact ccaagcaacc acaccgaaca gaacttcaca aaacgctctt gtctttgatc
    16381 ccatgttctc aaccactgct gtcaaaaaga gaaacggcta cccacaccga tctcgccgga
    16441 gccgcaaagt ttggcggggc cccagtctgt ccggtttata ctgagaatcg acacaatcgc
    16501 agtatttcat cttttcgtta atatcgaccc gagatgttga cgaacgatac taaacactca
    16561 agattcccat tttttccgca
//
LOCUS       DAOSUT010000087        16550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2161_length_16550_cov_9.889664, whole genome shotgun sequence.
ACCESSION   DAOSUT010000087 DAOSUT010000000
VERSION     DAOSUT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_16550_cov_9.889664"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLD05_09355"
     CDS             complement(<1..373)
                     /locus_tag="PLD05_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOP77688.1"
                     /translation="MAKQLVFDQSARQALAQGVSKLSMAVKATLGPRGRNAVLDKGWG
                     SPKITKDGVTVAEDIDLEDPNENLAARLVREAASKTNDVAGDGTTTATVLAEAIFREG
                     LKMVAAGADAMALSRGIQKAVD"
     gene            1120..1857
                     /locus_tag="PLD05_09360"
     CDS             1120..1857
                     /locus_tag="PLD05_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77689.1"
                     /translation="MMTWRLASTIEEWVKALRLVYQVYQQAGVIQSSKYQLRVTPYHV
                     LPSTDVFVAAERGTVLATASLIRDGLFGIPAETAYPDEIRDWRKQKRVFGEIACLAME
                     ATEQNELRMLLGLLRLAVQYARFTEHLDDVFLAVHPRHVPFYQRVLGFEAVGDIRHYP
                     GMTHLPMVLLVLDMTVLKTKHPRAHELLFGNRFPPEQLWPTRLPLDQRRLLEPFVDKR
                     FFPVPLSTDPGFEQCDPAYVTPAASPD"
     gene            complement(1827..3182)
                     /locus_tag="PLD05_09365"
     CDS             complement(1827..3182)
                     /locus_tag="PLD05_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOP77690.1"
                     /translation="MSLDQTPGAEDDRPVRRVANVRDFESFCKEVRRRPRLALDTEFI
                     AEDSYQPNLCLIQLAFDDELVVIDPFPLGSVRRFWEAIVEGNVETVVHAGRTDLQFCR
                     EATGRCPDRIFDVQIAAGFTSQEYPAGLANILQKYAHVSVTKHETRTDWRRRPLSDRQ
                     IEYALNDVRYLLAVRDQLESELKRLGRFEWFLEEVRYQYTECLTEDTEPWRRLAGCAG
                     LNRRELAILKELWLWREEEAARRNVPPRRVLRDDLLIELSRRGSGDPKRIYAVRGMTN
                     RGVQQLVPEIARRIQQALNTPREEWPEVINRTGIGQSPIVGQFLYAALASLCRKERIA
                     VGLVGGTNDLRDWFSYYVSGREGPPPLLARGWRASLIGDTLERLLSGKVGLRIHNCFD
                     DNPLELVPLSPETSSQGGNNVLPETLQQLPQDVETASVPKSLCGKPQVADQSGEAAGV
                     T"
     gene            complement(3630..6161)
                     /locus_tag="PLD05_09370"
     CDS             complement(3630..6161)
                     /locus_tag="PLD05_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77691.1"
                     /translation="MTRSNLVLGGLLIGLTLLAIFGCGRGSQPPPPPSPPPVAAPPPP
                     PPQPQPQPKEEKKEELPEDLSTWTKDHFILARKKQDKKIFDALEQLIADATAGQRGEE
                     AVSILRALLERPASPQPAGGPGGPYGSPMYGPGMASGMPEEGYSPEYGSPGPSPMPQP
                     QAAQAKDPNFEPDLLKAVASGIVKIATEASLAVIDDLLEGKIEVVNDRLAVETTLRGV
                     VEHIQQPMYEDLVIKVLCKPPRMAKTPGAAGPGGYPDMSPPASEYGSPYPSPDASMYE
                     SSTTGEYGSGYPGMAGGPQAKMRGDEIQRLLFDLAKDVLTQKIRMALAAHVMDPQTRQ
                     ADRQLMMPILTQPVPENLPVLLVFLQKGDSKQPLVMWIMDTLTDVMAAAFAAILGAED
                     PNGLNDLLALAQQRQIPGLQRGMPGQGGYPSPEGEYGSVPPGYAPGSPTTVATLPEMG
                     AYPGASPPGPEYSSETSVGPYPGQPGMRPGLSGGVMTTPQGLPKLVAAERRLAVRGLP
                     RDVALKVAPILWGPDLINFVDSRLMQARSLKDNPREILMAATLPRDATRARLASLFRA
                     NLENGPEPLAALGFGNNLFVDPGLVVVVKSLPREDLPKPSTSPTTPRTLGRRPPMGRP
                     GQYGQPGARPYPGGETDQYSPADSPYGGGPGGAQSQLTLTPKMRWMLMSESLSRSICK
                     KLKEAGDRLATIDPASLDTTSQKRPPEFQALHPNASVISEYHLDWPNSQLKQELPNVQ
                     FDPMRVHYIRLESHSNPNVVAGFYRRKLSTINQRTIADGIWLDGLQTLTDGKPRRQSI
                     DIFITHDAGEIGPQDQVDVVVELLSVEILSREAAAARIGGDTALR"
     gene            6755..7993
                     /locus_tag="PLD05_09375"
     CDS             6755..7993
                     /locus_tag="PLD05_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOP77692.1"
                     /translation="MNVAIFEDSRVNQLYPISVARPAFAISCGGYRLADLLPRLDRPL
                     FQYVRPHLQQIVAEDEKIPTVPRMLEGLWAFVNARLVPHVRIVDQIRGLLQKGSECAL
                     WHNNAVAAAILNFSEPCGWSQQPLDEFLLSEVQRRNVPTLDVRLPLFEYPHDVVKHHM
                     AIMRDNLEDRICQREYRQLQDGLFVGQDVSLGDYLTVNTQKGPVIIESHTRVGPCCYL
                     EGPVLIGGSAKLIENTALKDNVALGHTTKVGGEVEASTIEPYTNKQHHGFIGHSYLGS
                     WVNLGAGTCNSDLKNTYGLVTMEYNGQRVPTGMQFMGCIIGDYTKTAVNTGIFTGKVI
                     GVCCMVYGFVTTNVPSFTNYARLFGQMTEVPVEVAIAGQQRMFLRRNVVQRPCDIQLI
                     RDMYELTRHERRLGNEPLVL"
     assembly_gap    7994..8093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8094..9280)
                     /locus_tag="PLD05_09380"
     CDS             complement(<8094..9280)
                     /locus_tag="PLD05_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOP77693.1"
                     /translation="MDIPKPNAEDCLAYCEKLAAWCRAKALDMVRNSSLGADSKEIPV
                     HAGAGEPLLQLSPPNGLSPFRRIGIVGAGLMGSSIAAWHLAKGCQVVITDKSPAALET
                     VRQRVADELTRSGAVDKATCETRLADLQISDRLDDLATCDCVIESIVEDANAKTQLFR
                     QLESQLGPETPILSNTSTIPIGKLAMGLKHPERVCGLHFLHPVRSRDLVEVIPHSATH
                     RDIGKRVVEHALAVGKTPVITADIPGFVVNRLLFPYLNEGLQLIAEGFLPNEVDQAAA
                     EIGFLIGPIRIMDEIGLDTTWAAGRVLWEAFPERISPSPILVTMLKHNRLGRKNNRGF
                     FDYSSFSASEVEPSLDPETHKLIAPWMARPPLPQKLPVGMRLVLAMAFEAALILADNV
                     ADDP"
     gene            complement(9573..10952)
                     /locus_tag="PLD05_09385"
     CDS             complement(9573..10952)
                     /locus_tag="PLD05_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP77694.1"
                     /translation="MRFVGFVSGLCAVVVAGWVVLGTFGVHTAAAQVQPGSPPATTIP
                     GLPGIPGLPGTTPQKPEEKPVYPTVPSYLELSADDSLAKQAANVQRMLKEGNFTAVAN
                     GQDVFRDYYEKYFFPSWTKATNRSKLQQLRSQLIVTELGNYARAGGPPRDLLVQIAFN
                     TLSGYVKNDELDPAVRVAALLAIGDLNERERPTGGGMQPPVPLASALPFLVSILKDPQ
                     QLDALRLAALVGIERHCGAGIADTNFRDSEVIPLLVQLASDKNVPEGKDPKVHEWLRV
                     RAIDVIAITGQAGPNGQNIQMLLSFVTDDKESPKVRLAAARALGSFDYSQVQGVNLLT
                     IPQGLGKMVLDLCVADLQTVSQTGNWTKLETRLLAQYVSSAQQGLRGVRAAFAGKPED
                     QLISKLNRSLDEVTKALNDSLNARNPTMELVRLLPTALSNLNTALQGFAAADSGAPSA
                     NEGAQAGSP"
     gene            complement(11216..13219)
                     /gene="ligA"
                     /locus_tag="PLD05_09390"
     CDS             complement(11216..13219)
                     /gene="ligA"
                     /locus_tag="PLD05_09390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOP77695.1"
                     /translation="MGSNVAQEIEKLRDLIRYHDYKYYVEANPEISDLEYDRLMQRLR
                     ELEAAHPELITPDSPTQRIGDRPVEGLEQVRHRIPMLSIDNTYSPQEVRSFAQRVQKL
                     LKGETVEWVVEPKIDGVAVSITYEAGQLVRGVTRGDGFVGDDVTHNIRTVRGVPLRLL
                     TEAPPAVLEVRGEVFMTNSDLVRVNEEQKARGEAPFANPRNATAGSIRLLDPRLCAQR
                     RLRFLCHGLGYSEGFEVDTHMEFLHTVEKWGIPIPPWVTCFADIEKVLAYCEEQIERL
                     HELDFEIDGLVIKVNRFDQRERLGTTAKSPRWVMAYKFEKYEAKTRLLDIRVQVGKTG
                     VVTPVADLEPVTLAGTVVSRASLHNADEIERKDIRIGDVVVVEKAGKIIPHVVRVEKH
                     ERTRPLPKFHFPTTCPECHSTLVRDEGGVYIRCPNHECPAQIKERLRYYASRDAMDIE
                     GLGEKLIDQLVDRGLVRTYADLYRLKKDQLLGLERMGEKSASKLLANIEASKNRGLAR
                     LLAGLAIRHVGTKVAALLAKHFPTIEKLSAATVEELAEINEIGPIIAQSVYDFLHSER
                     GRRIIAELKECGVKMEEEIAVSRQSQPLAGKTIVVTGTLKRYSRHEIEELIERLGGHA
                     ASSVSRKTDFVLAGENPGSKLDKARSLGIPVLSEEEFEKLIAG"
     gene            complement(13434..13817)
                     /locus_tag="PLD05_09395"
     CDS             complement(13434..13817)
                     /locus_tag="PLD05_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOP77696.1"
                     /translation="MKVVHYESIPQQDVTMDGAKGCKIRHLISEADGAPHFAMRQFEV
                     APGGHTPHHKHAYEHEVFVLEGSGVVLQGEREFAIGPGSVVFVPANQVHQFRATGDRP
                     LKFLCLIPHAIQGSNVCCVVACSCD"
     gene            14268..14456
                     /locus_tag="PLD05_09400"
     CDS             14268..14456
                     /locus_tag="PLD05_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77697.1"
                     /translation="MQDIKQRREEIAKQLRDPKTRDLVISRLKTLMGIPPNEPLPNGT
                     PIITTLIRLENERQHSGA"
     gene            14674..14970
                     /locus_tag="PLD05_09405"
     CDS             14674..14970
                     /locus_tag="PLD05_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77698.1"
                     /translation="MLKERSEISPSESLHREVSDPSRHIEMVAQRRFDKSGYPALRRL
                     SCHCDGRRLVIRGQVSSYYEKQIAQASLRDLSENLEIVNEAEVLWPTYRVEGTI"
     gene            complement(15141..15797)
                     /locus_tag="PLD05_09410"
     CDS             complement(15141..15797)
                     /locus_tag="PLD05_09410"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOP77699.1"
                     /translation="MAYELPALPYAYDALEPHIDARTMEIHHTKHHQAYITNVNKAIA
                     GTEWEKLAVEDLVAAIHRVPEQIRTVVRNHGGGHANHSLFWTVIGPNGGGEPKGKLAQ
                     AIERELGGFEKFRAAFTAAAMGRFGSGWAWLSVDPNKRLVIEDTPNQNSPLMHGHIPI
                     LGIDVWEHAYYLKYQNRRNEYVEAFYHVINWGEVLRRYEAALAQPVPESAKHPLKTDT
                     "
BASE COUNT         3573 a         4780 c         4376 g         3721 t
ORIGIN      
        1 catcgaccgc cttttggatg cctcggctca aggccatggc gtcggcaccg gctgcgacca
       61 tcttgaggcc ttctcggaag atcgcctcgg caaggaccgt ggcagtggtc gttccatcac
      121 ctgccacgtc gttggtcttg gaagctgcct cgcggacaag tcttgccgcc aggttctcgt
      181 taggatcttc cagatcgatg tcttctgcga ccgtcacacc gtctttcgtg atcttggggg
      241 agccccagcc tttgtctaaa accgcatttc tcccccgcgg tccgagcgtg gccttaacgg
      301 ccatagacag tttgctgacg ccttgggcca gcgcttgtcg tgctgactga tcgaacacaa
      361 gttgtttggc cactggaagt tcctccttat tgactctcaa gagaacctta aggtatggct
      421 cagcttcgaa cgctgatctt cgtgttacga ctgaggtaag ttgtcaacct accacgcgcg
      481 gcctttctga cacccgtttt ctgcgacgtt ttcattcagc cgcgctcgcc caccattgtg
      541 tcgagaccac cggttacaaa agcggtgttc agctaccgcc cgtatcacag cccagcggtt
      601 tgtcccgtta ccctgagcaa gcggtgtgcc agaatggatt gaagatccta agttgtttac
      661 agatggacag ttatgaagat gaccgacact cgatgcctat ccgctgagtg attaatgatg
      721 ccaaaatggc agaacccact ttgaccaaac cctaccgcga ggctaaggga tactttcacg
      781 cggagtaaca actttcgagg ccgcgggacg ccctttttgg aatttcctga aaaaatggct
      841 cgaagcgttt gatttgttcc gcgtatgacc tagactttca gtaggtcgtg gtggccaaac
      901 tgattcgccg gatgccgatt ggtgcaaaag ttcttggaac gacgatcatc tcttgaaaag
      961 aattcttatt catagaacgt cgggttccgt ccgccgtgat ttcgccggcc aaaaaacgcg
     1021 agtctgtgcg atttagcaac ttagatgaag ctcccccaga aggggaataa tgtttcgagg
     1081 caatgtcccc ccgtgggacg tgtgctcgag gatatccgta tgatgacttg gcgcttggca
     1141 tcgacgatcg aagaatgggt caaggctctc cggcttgttt atcaagttta tcaacaagct
     1201 ggggttatcc agtcgagtaa gtaccaactt cgcgtcacgc cttatcacgt gctcccttcc
     1261 acggacgtgt tcgtggccgc cgagcgaggg acggtcttgg caacggcatc ccttattcgg
     1321 gacggtcttt tcggtattcc cgctgagacg gcgtacccag atgagattcg tgactggaga
     1381 aaacaaaaac gagtttttgg tgagatcgcg tgccttgcca tggaggctac cgagcagaac
     1441 gagttgcgaa tgcttctggg cttacttcgg ctggccgtcc aatatgcgcg gttcacggag
     1501 catttggatg acgtcttcct cgcggttcat ccccgacatg ttccctttta tcagagggtt
     1561 ctcggcttcg aagccgttgg cgatattcgt cactatcccg gcatgactca cctgcccatg
     1621 gtccttctcg ttctcgacat gacggtgctg aagacgaagc acccccgagc gcatgagctc
     1681 ctcttcggaa accgctttcc gccagagcag ttatggccaa cccgcctgcc tctggaccaa
     1741 cgtcgtctct tagagccttt cgtcgataag aggttctttc cggtgccgct tagtaccgac
     1801 ccgggctttg agcagtgcga tcccgcttac gtcacgccgg ctgcctcccc ggattgatcg
     1861 gcgacctggg gctttccgca aagggatttc gggactgagg ccgtttctac atcttgagga
     1921 agttgttgca gtgtctccgg taacacattg ttaccacctt gagacgacgt ttccggggac
     1981 agagggacca gttcaagggg attatcgtca aaacaattat gaatccgcag gcccactttg
     2041 ccgctaagaa ggcgctccaa cgtgtcacca atgagagatg cccgccaccc tcgagcaagt
     2101 agcggcggcg gtccttctcg tccggaaaca taataactga accaatccct gaggtcgttg
     2161 gttcccccga cgaggcccac tgcgatccgt tctttacggc agaggctggc caaggcagca
     2221 tataagaact gcccaacaat cggtgattgg ccaatacctg tacggttaat gacttccggc
     2281 cactcttccc gaggtgtgtt gagggcctgt tggattcgac gggcaatctc tgggacaagc
     2341 tgttgcacgc ctcgattcgt catcccacga acagcgtaaa tccgcttggg gtccccggac
     2401 ccccgccggg aaagctcgat caagaggtca tcgcgcaaca cacgtcgggg gggcacgttt
     2461 ctacgggcgg cctcctcttc ccgccagagc caaagctcct taaggatggc aagttcacgc
     2521 cggttaagtc cagcacaacc ggccaatcgc cgccaaggtt cggtgtcctc ggtcaaacat
     2581 tccgtatatt gatatcgaac ttcttcaaga aaccactcaa atcgccccaa cctcttgagt
     2641 tctgattcca actgatcccg taccgccagc agatagcgga cgtcgttcaa ggcgtactcg
     2701 atctgtcgat cactcagcgg acggcgccgc cagtcggttc gtgtttcgtg cttcgttaca
     2761 ctgacgtgag cgtatttttg gaggatattt gccagacccg caggatactc ttgacttgta
     2821 aaccccgcag cgatttggac atcgaaaatg cggtcggggc accgtcctgt tgcctctcgg
     2881 cagaattgaa ggtccgttcg acctgcatgc acaaccgttt ccacgttccc ttccacaatg
     2941 gcctcccaga atcgccgaac agaaccaaga gggaaggggt ctatcaccac aagttcatca
     3001 tcgaaggcaa gttgaattag gcagagattc ggttggtagc tgtcttccgc aatgaattcg
     3061 gtatcgagtg ccaaacgcgg ccgacggcgg acttctttgc agaagctttc gaaatcacgg
     3121 acattagcga cccgccgtac cggacgatcg tcttccgcac cgggcgtttg atccaaactc
     3181 acgtttgacc ccccatgact gcagcgagca ttaagacacc cttttaatga ggtcgggccc
     3241 ccacatagga gaaataccca cagggagggc cggtgtcaaa tcacttttac cccaagaagg
     3301 ccaatcggcg ttcttccgct ccttttcaga attccccaaa ttctcaagat agcattaact
     3361 caaaaacagg gagcggcacc caatgtttgc ggagaccacg cccctctctg tcctctcaat
     3421 tagagcctat cccataatct cccctcgccc gttcggcggg agaaggggcg ggggtgaggg
     3481 tggctcgaat cgctgccggt tttctcaatc gattctcacg ttcgtttgcc ctcgccctac
     3541 ccctctccca gagggagagg gggtttttgg gatggactct tggctttcgc gtgatgttgg
     3601 gttctcgacg ttttccgtac gatagccgat taccggaggg cagtatcgcc accgatcctg
     3661 gcggcagcgg cctctcgcga aagaatctct accgataaca attccacgac cacgtcaacc
     3721 tgatcctggg gaccgatttc gcccgcatcg tgtgtgatga aaatatcgat ggactgtcgt
     3781 cgcggctttc catccgtcag agtttgcaac ccatcgagcc aaatcccatc ggcaatcgtt
     3841 cgttggttaa tcgttgaaag ttttcgccgg tagaagcccg ccacaacatt ggggttgcta
     3901 tggctctcca agcggatgta gtggacccgc atgggatcaa actgcacatt ggggagttcc
     3961 tgctttaatt ggctattagg ccaatccaaa tgatattccg agatgacact ggcgttggga
     4021 tgaagagcct ggaactccgg cggtcgtttt tggctggtgg tatcaagcga agcgggatcg
     4081 atcgttgcca gccgatcccc tgcttctttc agtttcttac agatggagcg gctgaggctt
     4141 tcggacatga gcatccatct catctttggg gtgagggtta gttgcgactg agccccgccc
     4201 ggcccaccgc catagggcga gtctgccggc gaatattgat ccgtttcccc gcccggatag
     4261 ggtcttgccc ccggttgccc gtattgcccc ggtcggccca tcggtgggcg tcggcctagt
     4321 gtccgcggtg ttgtgggcga ggtactcggt tttggaagat cctcccgggg caggcttttc
     4381 actaccacca ccaatcctgg atcgacaaac agattgttgc cgaaaccaag ggcagccagc
     4441 ggttcgggac cattctcgag atttgcccgg aacaacgaag ccaaccgtgc acgggtcgca
     4501 tcccgtggca gagttgccgc catcaggatt tcccgcggat tgtctttcaa cgatcgggcc
     4561 tgcatcagtc gtgaatcgac gaagttaatc agatcaggcc cccacagaat gggtgcaact
     4621 ttcaaagcaa cgtcccgagg gagtccacgg acagccaacc gtctttccgc tgccacgagc
     4681 ttcggcaggc cctgcggggt tgtcatcaca ccgcctgaca gtcccggtcg catgccgggt
     4741 tgtcctggat aaggccccac agatgtctcc gaagaatact ccgggcccgg tggtgaggcc
     4801 cccggatacg cgcccatttc cggcaaggtt gcaaccgtcg tgggactacc gggagcgtaa
     4861 cccggcggca ctgatccgta ctccccctcc ggtgatggat agcccccttg ccccggcata
     4921 cctctttgca aacccggtat ctggcgttgc tgcgccaggg ccaacaggtc attcaggccg
     4981 ttgggatctt ccgcccccaa aatggctgca aaagccgctg ccatcacatc agtcagtgtg
     5041 tccatgatcc acatgacaag cggttgcttg ctgtctccct tctggaggaa cacaagcaac
     5101 acaggcagat tttcgggaac cggctgcgta aggatcggca tcataagctg ccgatcagcc
     5161 tgtcgggtct ggggatccat cacgtgcgcc gccaacgcca tgcggatttt ctgtgtaagc
     5221 acgtccttgg ccagatcgaa caaaaggcgt tgaatctcgt cgcctctcat cttggcttgc
     5281 ggtcccccgg ccataccggg ataccccgac ccatattcgc cggtcgtact cgactcgtac
     5341 atggacgcat ccggagacgg atagggactg ccgtattccg acgcgggagg cgacatatca
     5401 ggatagccac ccgggcccgc tgcacctggt gtcttcgcca tgcggggcgg tttgcataat
     5461 accttaatca ctaggtcctc atacattggc tgctggatat gctccacgac gccccggagg
     5521 gtcgtttcca cggcaaggcg atcattaacg acctcgatct tcccttccaa caggtcatca
     5581 atgacggcaa ggctggcctc agtggcaatc ttcacgattc cggaagccac ggccttgagc
     5641 aagtccggtt caaaattcgg atccttggct tgggccgcct gcggttgagg catgggcgat
     5701 ggtcccggag agccatactc tggtgaataa ccctcctctg gcatcccgga ggccattccc
     5761 gggccgtaca tggggcttcc ataaggtcca cctggtccgc cggctggttg cgggctggca
     5821 ggtcgctcga gcagggcccg caagatggac acagcttcct cccctcgctg cccagcggta
     5881 gcgtccgcga taagctgctc caacgcgtcg aagattttct tgtcttgctt cttccgggca
     5941 aggatgaaat gatccttggt ccaggtgctt aggtcttcgg gcaattcctc cttcttttcc
     6001 tctttcggct gaggctgagg ctgaggtggc ggcggaggcg gcgcggcaac cggtggcgga
     6061 ctcggtggtg gcggcggttg gctacctctc ccacacccaa aaatcgccaa aagcgtcaac
     6121 ccaatgagta gcccccccag aacaagattg gaacgagtca tagccaccct ccgttccacc
     6181 aaacagactg tttgcaaaca tggtaacggc ccattcggtt ggccgtctga cagtcatatt
     6241 aaccgttctc ctcgatctcg taccccccac cgagacgacc ggccggtccc tccgatgtcc
     6301 tgaacgtcat tgtaacgaaa actcttccag atgtttagca attcgtcgcg tcctcggcgc
     6361 cgaaggggag catcggctcg cctcgaattc tgggcacccc aaacgattca aaacctgccg
     6421 gaattatgac accagttttg acgggagaaa tcaccgcgag gtccaagtgg tcatgggctt
     6481 cacccaaagg ggtttgtcac cgcagccacc tgtgcggcct atactctgta ccacatggct
     6541 acggcgtcgg ggcggcacgc taccccaacc gttgggagtt cgctttattt cgccccgcaa
     6601 tcttacccga tggtggtatc cattgtccat ctccggcatg gtcaccaaat tggctttgac
     6661 aagacgcgtc caaaaaaagt aacgtttaac agggtccgtc gatacttttt ggaggtaggc
     6721 cgccttcggc tcatcctatt aggagtacct ctccgtgaac gttgccattt tcgaggattc
     6781 acgcgtcaat caactatatc caatttccgt ggctcggccg gcctttgcca tctcctgtgg
     6841 cggttaccgg cttgccgatc ttctgccaag actcgatcga ccattgtttc agtatgtgag
     6901 gccccacctg cagcagatcg tcgccgagga cgagaaaatt cccaccgtgc ctcgaatgct
     6961 ggaggggtta tgggcgtttg tcaatgctcg cttggtaccg cacgtgcgaa ttgtggatca
     7021 aatccgcggg cttctgcaaa agggttccga atgcgcgctt tggcataaca acgctgtggc
     7081 ggcagccatc ctcaacttct ctgaaccgtg tgggtggtcc cagcagccgc tagatgagtt
     7141 tctgctgagt gaagtccaaa gacggaacgt ccccacactg gacgttcgct tgcctctttt
     7201 cgagtacccc cacgatgtgg tcaaacatca catggccatt atgcgtgaca acttggaaga
     7261 tcgcatttgc cagcgggagt atcggcagct tcaagatggc ctcttcgtcg gccaggatgt
     7321 cagcctgggc gattatctga cagtcaacac gcaaaaagga cctgtcatta ttgaatcgca
     7381 tacccgggtg gggccatgct gttatttgga aggcccggtc cttattggtg ggtccgcaaa
     7441 gctaattgaa aacactgccc tgaaagacaa tgttgccctc ggccatacga caaaagtcgg
     7501 gggagaggtc gaggcctcca ctatcgagcc ttacaccaac aagcagcacc acggttttat
     7561 tggccacagc tatctgggga gctgggtcaa cctaggggca ggaacctgca atagtgacct
     7621 taagaatacc tacgggctgg tgacaatgga gtacaacggc caacgtgtcc ccaccggaat
     7681 gcagttcatg gggtgtatca tcggcgatta cacaaaaaca gcagtcaaca cgggcatctt
     7741 cacgggcaaa gtcattggcg tttgttgcat ggtttacggc tttgtcacaa ctaacgtccc
     7801 cagtttcacc aattacgccc gactctttgg tcagatgacc gaggtccccg tggaagtcgc
     7861 cattgccggt caacagcgga tgttcctccg tcgcaatgtc gttcaacggc catgcgacat
     7921 ccagcttatc cgcgatatgt atgaactgac ccgtcacgaa cgccgattgg gcaacgaacc
     7981 gctcgtcttg tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggatcgt
     8101 cggcgacgtt gtcagccaaa atgagggccg cctcaaaggc cattgccagg acaaggcgca
     8161 tgcccacggg taacttctgc ggtaatggtg gacgggccat ccatggggca atgagtttgt
     8221 gagtttctgg atcaagtgat ggctcgactt cgctcgcgga aaaggacgaa tagtcgaaga
     8281 acccgcggtt attttttcgg cccaatcggt tgtgcttcag catggtgacg agaattggtg
     8341 aagggctgat cctctccgga aatgcctccc aaaggactcg accggcagcc caggtggtat
     8401 ccaaaccgat ttcgtccata atccgaatag gcccgatcaa aaagccaatt tccgcagccg
     8461 cctggtcaac ctcattagga aggaacccct cggctatcag ttgtaggcct tcgttaaggt
     8521 agggaaaaag aagacggttg accacgaagc ccgggatatc tgctgtgata acgggagttt
     8581 taccaacagc cagagcgtgc tccacaaccc gtttgccaat gtccctgtgt gtggccgaat
     8641 gggggatgac ctcaacgagg tcccttgaac ggaccgggtg caagaaatga agtccgcaga
     8701 cacgttctgg gtgtttcagc cccattgcca gtttgccgat gggaatggtg gaggtattcg
     8761 acaggatcgg cgtctctggc ccgagttgcg actcaagctg cctgaatagt tgcgtcttgg
     8821 cgtttgcgtc ctcgacgatg gattcgatga cacaatcgca cgtggccagg tcatccaagc
     8881 ggtcagaaat ctgaaggtcg gcaagccggg tttcacaggt tgctttatcg accgcccccg
     8941 atctcgtcaa ctcatcagcc acccgttgcc tgacggtttc caaggccgct ggagacttgt
     9001 cggtgatcac cacttggcat ccctttgcaa ggtgccacgc ggcaatgctg ctgcccatca
     9061 gaccggcgcc aacgatgcca attcgtcgaa agggcgagag accattcggc ggcgacaatt
     9121 gcagaagagg ttccccggca cccgcgtgga cagggatttc cttgctatcg gccccaagac
     9181 ttgaattacg caccatgtcc agcgccttgg cacggcacca ggctgccaac ttctcgcaat
     9241 aagctaaaca atcttccgca ttgggtttgg gaatatccat ctcgtgggat tgtctagaaa
     9301 ctgggccgaa ggatgcctcg cccctccaac caggtgaaag cctcccacca tggtccgtcc
     9361 acgcaggaac tctcacagca tttcgcagat acaggctatt ctatcagagg aaggtccctc
     9421 ggaatcagct cggcgcaacc aagtcgcggt ggaaaaggat gaaagtccac gggccgaata
     9481 caaaccaaca aagcccccca ctgcctttta ccacccgtga tcggagatca ttgagcaggg
     9541 gccctccgtg gtaagcaaaa caccgggcac gactagggtg aacccgcttg cgccccttcg
     9601 ttggcggacg gtgcgcctga atcggccgca gcaaaacctt ggagggccgt attcaggttg
     9661 ctaagcgcgg tgggaagcaa gcgtaccaat tccatcgtag gatttctggc attcagcgag
     9721 tcattgaggg ccttggtaac ttcatcgagt gagcgattca gtttgctgat caactgatcc
     9781 tcgggtttac cggcaaatgc tgcccggaca ccacgcaagc cctgttgggc cgagctgacg
     9841 tactgggcga gtaatcgcgt ttccagtttc gtccaattgc cagtctgaga caccgtttga
     9901 agatcagcca cacaaagatc gagcaccatc ttgcccaggc cctgcgggat ggtcaacaaa
     9961 ttgacccctt ggacctgcga gtaatcgaaa ctgcccaatg cccgtgccgc tgccaatcgc
    10021 accttcggcg attctttatc gtcggtgaca aaactaagca gcatctggat gttctgtcca
    10081 ttcggaccgg cctgcccagt aatcgcgatg acatcgatag cacggacccg cagccattcg
    10141 tggactttcg gatctttacc ttccggaacg ttcttgtcac tggctagttg gacaagaagt
    10201 gggatcactt ccgaatcgcg gaaatttgta tcggcaatcc cggcgccaca atgacgctca
    10261 atccccacca aggcggccaa tcggagggca tccagctgtt gcggatcctt caatatcgat
    10321 accaggaaag gcaaggcact ggcaagaggc accggaggtt gcatcccacc gcctgtcggt
    10381 cgctcacgct cgttgagatc gccgatcgcc aaaagggcgg caacccgcac ggcaggatcg
    10441 agctcgtcgt tcttgacgta accactcaac gtgttgaatg cgatttggac gagcaaatca
    10501 cgcggcggac caccggcccg ggcgtagttt cccaactccg taacaatcaa ttgcgatcga
    10561 agttgttgca gtttcgaccg atttgtcgcc tttgtccaac tcgggaagaa gtacttttcg
    10621 taatagtcac ggaagacatc ttgaccattg gccaccgccg tgaagtttcc ttccttgagc
    10681 atccgctgaa cgttggccgc ttgcttggcc aaagaatcat ccgcggaaag ctcaagatag
    10741 ctcggcaccg tggggtagac gggcttttcc tcaggttttt gcggggtagt ccccggcaaa
    10801 ccgggtattc ctggcaaacc gggaatggtt gttgcaggtg gcgaaccggg ctgcacttgt
    10861 gctgcggcag tgtgcacccc gaacgttccc aaaaccaccc agcctgccac aacgacggca
    10921 caaagaccgc tcacaaatcc aacaaatcgc atggcgagat ctcgctgaag acaatgcttg
    10981 atcagaactg atcttccacc ctctaaagta cttaccgggg cattacggtg tcaagcaata
    11041 ggcgttttgg ataactccgg tttataccgc acctaccatt caggttccat ccccagctta
    11101 cgccgcagac tcgagactct tggcttatac acggccaaaa aatagtctct caaacgcccc
    11161 cgttcaaaac cgtaacagcc tctagcgaag ggtaataggg ggcactggtg taccgctagc
    11221 ccgcgatgag cttttcgaac tcttcttcac tgagaaccgg gatacccaac gaacgggcct
    11281 tgtccaactt gctcccgggg ttctctccag ccaggacaaa atccgttttc cgcgagacac
    11341 tagacgccgc gtggccacct aagcgttcga tgagctcctc aatctcgtgg cgcgaatacc
    11401 gcttcagcgt gcccgtcacc acaatcgtct tgcctgccaa tggttgcgac tgccgcgaga
    11461 ctgcaatctc ttcctccatc ttcactccgc actcctttaa ttccgcaatg atgcggcgcc
    11521 cacgttcact gtggagaaaa tcgtaaacgc tttgggcgat gataggccca atctcgttga
    11581 tctcggctaa ctcctcgacc gttgccgcag aaagtttttc gatcgtggga aaatgctttg
    11641 cgagaagcgc agcgacctta gtaccaacat gacgaatggc caaccccgcc aaaagccgtg
    11701 ccaaaccacg gttcttgctg gcctcgatgt tcgccaacag cttactggcc gacttctccc
    11761 ccattcgctc caagcccagg agttggtcct ttttcaaccg ataaaggtcg gcgtacgtcc
    11821 gaaccagtcc ccgatctacc aactgatcaa ttagcttttc gcccagacct tcaatatcca
    11881 tcgcatcacg gctggcgtaa tagcgcaatc tttccttgat ctgagcggga cactcgtgat
    11941 tgggacaccg aatataaaca ccaccttcat cccgcacgag cgttgaatga cattccggac
    12001 acgtggtagg aaaatggaac ttgggcaggg gccgagtcct ttcgtgcttc tccacccgga
    12061 ccacatgagg aatgatcttg cctgcctttt ccacaacaac tacgtcgcca atacggatat
    12121 cttttcgctc aatctcgtcg gcattgtgca ggctggcccg gcttaccacc gtgccggcca
    12181 gggtcacggg ttccaaatcg gcaacaggag tgaccacccc agtctttccc acttggaccc
    12241 gaatgtctag cagccgggtc ttcgcctcgt acttctcaaa cttgtacgcc atgacccagc
    12301 gaggactttt tgccgtcgtc cccaaacgct cacgctggtc aaagcgattg accttaatga
    12361 cgagcccatc aatttcaaaa tccagctcgt ggagacgttc gatctgttcc tcgcaatagg
    12421 ccaagacttt ctcgatgtcg gcaaaacagg taacccacgg aggaatggga ataccccact
    12481 tttccaccgt gtgcaggaac tccatatggg tgtcaacttc aaagccctct gagtagccca
    12541 gcccatggca gaggaatcgc aggcgtcgct gggcacacag acgcgggtcg agtagtcgaa
    12601 tgctaccggc cgtggcgttg cgcggattgg caaaaggggc ctcaccgcgg gccttttgct
    12661 cttcgttcac tcgaaccagg tccgagttcg tcatgaatac ttcgccgcga acctccaaca
    12721 ccgcgggcgg tgcttcagta agaagcctga gcggtactcc ccgcacggtg cggatgttat
    12781 gcgtcacatc gtcacccaca aaaccatcgc cgcgcgtcac gccgcggacc aattggccgg
    12841 cctcataggt aatcgagacg gctacgccat cgatctttgg ctcgaccacc cattccacgg
    12901 tttcgccctt gagaagcttc tgcacccgtt gggcaaagct cctgacctcc tgcgggctat
    12961 aggtgttgtc gatcgacagc atgggaatcc gatggcgaac ctgttcgaga ccttcgacag
    13021 gtcgatcccc gatgcgttgt gtgggactgt caggggtaat caactccgga tgggcggctt
    13081 ccaactcccg cagtcgctgc atgagccggt catattccag atcagaaatc tctggattgg
    13141 cttccacgta atacttgtaa tcgtggtacc ggataaggtc gcgaagtttt tcgatctctt
    13201 gggcaacgtt cgatcccata cgagcaggcc taatctctca caagttcttc tcttgccgtg
    13261 aatagctcgc cgtccgagtt gggcgtaatg acatccttga atttaccggc cccaaacttg
    13321 cgaagaaaca gggagaaggg tttcgctgct aacttctcac cgctggccga accgaaacct
    13381 taccacaaaa aatcgtgcga cgtttagacc gacccaggtc accatcggcg ccctcagtca
    13441 caactacagg ccaccacaca gcacacattg gacccttgaa ttgcatgggg gatgaggcag
    13501 aggaacttca acgggcggtc acccgtggcg cgaaactgat gcacctgatt agccggcacg
    13561 aagacgacgc tgccaggacc tatcgcgaat tcacgttctc cctgaaggac aacccctgac
    13621 ccctccagaa cgaagacctc atgttcgtag gcgtgcttat ggtgcggggt atgaccaccg
    13681 ggtgcgacct caaactgtcg catggcaaaa tgtggagcgc cgtccgcttc gctaatgaga
    13741 tggcggattt tacacccctt cgcaccatcc atcgtgacat cctgctgggg aatgctttcg
    13801 taatggacca ctttcatcga atctcactcc caggttggga caacgggtag cttctcattt
    13861 gcgtccgtga ttgccgaaga atcgccagac cggtatcttc ctatccctcc atggtagcgg
    13921 aaagggaaaa tcgggtcacc tcaaaaaccg cctcttctta tgacacattg gttcgcccgg
    13981 tggagcaata tcccactcac ccgcacgcga tacgtgcccg cctttaggaa aatatggcaa
    14041 ctacagtaaa taggtaaact tttccgcgat accagtcgat aggggtatat ggtaagcgat
    14101 tcgattacgt tggccagttc ggccggcggc tgtagttggc gacctgggag ttgttgtctt
    14161 tcaactccgt cggcacgtgt tcgaacccgt gatggcctta cgcaccaaat tcgcccggtt
    14221 tgttggcgtg atgtctggac ctcgtaagga atgggaggtt cctagcgatg caggacatca
    14281 aacaacgacg ggaagaaatt gccaagcaac tgcgtgatcc gaaaacccgc gatcttgtca
    14341 tttcacgact gaaaacatta atgggaatac ctccgaacga gccgttaccc aacggtacac
    14401 cgattatcac caccttgatc agattggaga acgaacgaca gcactctgga gcataacgaa
    14461 tgccggggaa ttgaccgctg gtcccccgtg caaatgatca tttgcgaaac aaagtctcgg
    14521 ggctgtatgg gccgtttggc cgagaaaggt caaccggcga caaccttctg ggacacttgg
    14581 gtttgggaga gggcggaaga gggacctccg ccgaaggtga aaacggagtg gcatccttgg
    14641 agcgtgcttg caaccctgag gtggggcggt gtcatgttga aagaacgttc tgagatttcc
    14701 cctagtgagt cgcttcatag ggaagtgagt gatccctcac ggcacatcga aatggttgcc
    14761 caaaggcgat tcgacaagag cggttacccg gccttgcggc gactctcgtg ccattgtgac
    14821 ggtcgtcggc tcgtgattcg tggacaggtc agcagttact acgaaaagca aattgcccag
    14881 gccagtctgc gtgacctttc cgaaaacctc gagatcgtca acgaggccga ggtcctttgg
    14941 cccacgtatc gtgtcgaggg tacgatctag ttcctcgcat ttcaaagtta gatccccttg
    15001 ccgacccaaa caaagccatc ggcaaaagcc gcgcaagccg cctctcgcgc ccgatcaaca
    15061 aaaggaaagc cggcgcgtca cgcgccggct ttaccttaag gtgatcaacg atcacccggt
    15121 cgtggggatg ggctgtattt ttaggtatcc gttttaagcg gatgctttgc ggattcgggg
    15181 accggttggg cgagggccgc ttcgtagcgt ctgaggactt caccccaatt gatcacgtgg
    15241 tagaaggcct cgacgtattc attcctccgg ttctggtatt tgaggtaata tgcgtgctcc
    15301 caaacgtcga tgccgaggat tgggatatgc ccatgcatta gggggctatt ttgattgggc
    15361 gtatcctcga tcaccaatcg cttgttcgga tcaaccgaga gccatgccca accgctgccg
    15421 aaacgcccca tggccgcagc cgtgaatgcc gcccgaaact tctcaaatcc tcccaactct
    15481 cgctcaatag cctgggccaa ttttcctttg ggttccccgc caccattggg cccgatcacg
    15541 gtccaaaaaa gggagtgatt ggcgtgcccg ccaccgtgat tccgcaccac agtgcgaatc
    15601 tgctcaggca cccggtgaat ggccgccacc aggtcttcta cagccagttt ctcccattct
    15661 gtgccagcga tcgccttgtt cacgttggtg atgtaagcct gatggtgctt cgtgtggtga
    15721 atttccatcg ttcgggcatc aatgtgtggt tccagtgcgt catacgcgta ggggagagcc
    15781 ggtaactcgt acgccatgtg tcaactcctc gtttgtcaat gaaagtctcc gcttgcaacc
    15841 cgcgatggct gcgtccctcc gaccagcctt ccttttctcg cggagctgcc tagatcgaca
    15901 gtcatggcca ccatattggc catttatgtc ttacttattt gtctaaataa taaccaaccg
    15961 gtcctgaaag acaaggccac gaatttctgg gagttgaacc aatacccgac gtggaccgga
    16021 gctcggggat taaggagcct atcccacatc ctcccgtcgc ccgttcggcg cgagaggggc
    16081 cgggggtggg ggtggctcaa atcgctgctg gttttctcga tcgagccgga ccgtgtcttg
    16141 ccctcacccc aaccttctcc caaacggaga ggcaattttg gatacgctct aagttcgaat
    16201 gcgcagcttt tgcgcacatc tgcccgagtg gcatttcaca aggcttatca acgtggttca
    16261 gttgcttacc caagtccgtt cgaaacgttg ccacttgcac actgactttg gccctccgga
    16321 cgtcccaaaa aggacaaatc ggtaaaatcg tcaaatcgcg cgcctggaag cttggggtaa
    16381 ctggccaatt ttggggattt taggaggaag gataattcgc cgctgagtat tatagaaact
    16441 atgcggcact cgttcctggt gtctcggcct gtgactcttc gctagtaagc cggggcaatt
    16501 atccctgatc gcgtaaatcc gtcactacac cttttgagtt ggcacctggg
//
LOCUS       DAOSUT010000088        15970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2251_length_15970_cov_10.397989, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000088 DAOSUT010000000
VERSION     DAOSUT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15970
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_15970_cov_10.397989"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLD05_09415"
     CDS             <1..1029
                     /locus_tag="PLD05_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77700.1"
                     /translation="EFVFINRSCGAEQPFNEQEPAVAQLQQVLFPFPGAINRLNSSTM
                     KFTPLVKTGRITGLVNYDDIFRMDFLTGSRELNTNRPLRPTRTEYVLAATIEGKLPEM
                     KITKSEESNPEPGASSNKVEELADKQHDVRVILVADIDMLHETFFRLRQRQDLPGLDL
                     KLSFDNVTMVLNLIDRVSGDDRFIDIRNRRPKHRTLTTIERATEAARARTAEERQKLQ
                     EAYNQIEKEEREKLEQSVKKLEEDMKKQNISLDEILRRVAIAQEQGQRRLDARMEEER
                     QRRDRALERIETELTLYVRRLQNFYKMVSVIIPPLFPLGLAVIVFVWRRARELEGVPT
                     SRRVRKRF"
     gene            1187..3091
                     /locus_tag="PLD05_09420"
     CDS             1187..3091
                     /locus_tag="PLD05_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOP77701.1"
                     /translation="MNETKKTFAFVAVAAVVVLIAWWTRYTPPVTTTADMRGKPLFPG
                     FTDALAAKSLEIFEYDPTSVKIKNFKVAQINNRWSIPSHENYPADAKDHLAQAATSLI
                     GLTILDVASESPTQEEVALYGVVEPDEKIIKTSVRGIGKRVIFRDQGDKVLADLIIGN
                     KVPDKEELRYVRIKGQDPIYVVKLSTDKFSSEFGDWIEKDLLKLNPWDIKDVQLHDYS
                     FDSLTQTLAPRSQVILGYDDLGSPRWKLVQNLVFDGDQGTWKPQSLAEDEELDTSKLD
                     SMRTALDDLKIVDVRRKPEGISASLSADGTLAANRETAASLAQAGFYLVSARDFYKIF
                     PMIGKKELKPNDVEVISSEGEIRVGMKDGVRYLLRFGQVVAGGSSGQEGEGGAGVNRY
                     LFVMAEFDPDLIPKPELQPLPEVPSEGQSPAATTGTSQSQSAATEEAQSGTSVTQGSA
                     PPEQSSGSEEAKSQTSEEKPAEQQKKPEDIKAERERIEKENKRKQDEYEEKLKKGQER
                     VKELNARFAEWYYIISDDVYRKIHLGIGDIVKKKEKKEEETKTSDSSTPSPTEESKKA
                     EATSSGDTSQAAKESPQETKPVESTPSPEAPAGASPPAPPSEETAPATGAAEQQPQGG
                     DSSPEPMPMQ"
     gene            3286..4029
                     /locus_tag="PLD05_09425"
     CDS             3286..4029
                     /locus_tag="PLD05_09425"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOP77702.1"
                     /translation="MGPNAVIGEFAGQIAAELDEFCRSTVERVCGTEYPDDMFRLADR
                     LGMEIIVDGGESHRARCLRIQRGEKSQWLLIVRDEPRWERMQWAIAHELGESLAGQAI
                     MKLGWDVEDLQPVHREWLANEIAKRLLLPFPEFLSEGAALEWDLYRLKEQYPNASHEV
                     IARRMLDAPRPIVITIFDNGRLYWRKSNSLIASPPLTQQEKACWRAAHETGQSVRYQN
                     AVESIVCWTIHENGWQREILRTESPWLEE"
     gene            4259..5746
                     /locus_tag="PLD05_09430"
     CDS             4259..5746
                     /locus_tag="PLD05_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP77703.1"
                     /translation="MTLRTKQTMSGVRRGLVGWLFLVASGISAWASAADLIHPQRLTP
                     YGLEVAWFQQVQVDPARGRVTEWLLDRGTLFVVTNTAVLQAFDAETGRSLWATPVGRR
                     ELLTLKPTAGEHLVAVVNGSTIYVLNRYTGVILWSRQTEGAVGAGPVLAPTHVYVPLI
                     RGKIEAYRLEWANPETALAARTAEFPQDQATSPATAGMTTQSSEQSNGAESVTPIAMK
                     PVAKEEFRLSTQPRPGHFVMSWGRALVEPRLTFADRNRERLAWPTDKGLLFVGELDVR
                     SELRFSVQYFFQISGGDIVAPPCIRPGDPSNPADIQRMLYVAATNGFVYALREWDGKL
                     LWRFSSGEPIKESPALIGDDLFVTTQTGGMYRLNAVSGDLIWFARDLRRFVSASDQRV
                     YAVDGLNNLVIVDLQTGSRVGQLDVSRYPLRYANSETDRIYLASDTGLIQCIREARLP
                     QPIKHVVPITPLKEQKPAKPAAAQPAETPAQPQPPPQPAGENPFQ"
     gene            complement(6103..6705)
                     /locus_tag="PLD05_09435"
     CDS             complement(6103..6705)
                     /locus_tag="PLD05_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77704.1"
                     /translation="MDISRREGKPTARPPAKGDDKPPVVKKRRVFSLSHVGEGLVLLI
                     ALAPVFLIGAVAGSKSLWTNWLEKIGVRESWGNETSHVPPAAVWQGDGVADFGQIAVY
                     TYDPASGLVRSTCFRLHAVMAFSDQHEFENFIRRTGYVIRDEILVTVRASPLSEVLDP
                     DFLSRKITARVNRILGADTVRSVQISDLNVTEYTPADTRK"
     gene            complement(6854..>7954)
                     /locus_tag="PLD05_09440"
     CDS             complement(6854..>7954)
                     /locus_tag="PLD05_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOP77705.1"
                     /translation="HVIPTLGQHEPHSREQNARMFGSIPHERIHAHHWINTVVRLGEV
                     PANFVEQVTHGAANWAIPIELNSMLMQEPWDLVINIGHVVPHEVLGFANHNKNYFIGL
                     AGKGTICASHIAAACCGIENNLGTLITPVRACYNKAENEFLGSLPDFYIQIVMARDPE
                     DRLVHTGIYVGDDLETYLMAARQSREENIKVFEEPLQKVVCVMQEDEFVSTWVANKAI
                     YRTRMAMADGGELIIIAPGLKRFGEQEEVDRVIRKYGYVGTQRVMELYRQNQDLQELA
                     HATAHLIHGSSEGRFRITYAPGHLTKEEIEGVNFGYADLQETLRRYPPDKMREGWNTT
                     PDGETIYFIPTPSAGLWATRKKLYERPAGYEC"
     assembly_gap    7955..8054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8376..12380
                     /locus_tag="PLD05_09445"
     CDS             8376..12380
                     /locus_tag="PLD05_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77706.1"
                     /translation="MKPEKSASPVEVILGYLNFSQGVRDPRFFAAWNRLWTLREIAFL
                     SQPSEAQTQERPELLHRYLDNELSRLRRENPAFSDTAQAEYVLEFLFERLIHGYRQFH
                     RDILAHLTLDDYCQPFLLARFCEAILAELHEAELDGKADDNEMLEATIRRLNDYLGYR
                     PVPTLEGKKRHKPYEHEFVCPIPLWIRGAGAAVGPYQRLIETALGILRETSAELCRAS
                     YFDFEQLEEIVLDPRGYDMEHPVHQRPNYLYGLWDPRKLNDRGYYCRFVLHDVHLNSI
                     LARVLEGPADDYEERLFEGAAVLAGTILMASGISGDRPGAILSSVQIDKWLQGIALVR
                     DQFYIELLGRCTGERRQRLESEMQRLRQPFGATRQALNQKLAARRSQELYVVSLATCF
                     AKMGAVEEARYWMQQIHSGAARIRCEIRCQLGECEWLIRNNRRTEALKTLSGVIELIH
                     RGIECGALIDPWCILGFGCLFPIFEHLREGVFDYRVEQLVGLVRLVFQRLAALRRAAA
                     ASGDEAVDEAAENLAAELADWWDQFATTAVADANSFLGRDECTAATVVSRILKRWREE
                     PEAAQSLAFWKEHAGSLQSAASYVLLVETLLQHRDTTAAMALLVDWASRADEVGLGVG
                     EQSFQVMALRWFQTIWTEQKDIKDRWKWTQKFIDYLEANCESLFRVPNLSEFFKRAGS
                     PVIRSEDEENGDDDVLSAAYEGVIFRDSAADGHEGDTLDDEISDSELAPLLGEISDRC
                     NFLSATTLIRYDALLASFRAISEVPDSATILMTWYRDAQEAEERLLQLAEEIWRYTLV
                     PPSPSPEGMMEFERQRQIRDALVDRVIQTAINVLSLRYLLRAIINVEATEDESQQPQW
                     DTYYPRLLRAVLYGDEAAFHQLMESFAKVIVTRPILYFSLVRGGNPRTYFLARGTHRA
                     LGLLAYLIARAGWVNAAMGLLSLVKSAERHQSEGKGKVSEIEHLFEAVAEGIFLALVF
                     AKRNGAKRKEGRLSRQETEDLVADLHAVCNGLLEIWAVIIHEIYFSTVETAPFRRSWT
                     QIRQFIRAFGQEIFTPMFMAHPNLRAIMATGTHHWLKMVKESGDAQWPRLVEALRSGE
                     LKLKKAAEYLRLIIDVILENYEDYLDYNSMTTQSDYGQNLYILLDFLRLKCEHQRRAW
                     MLKPFLLAHRVLVRLELWEAAEIFEEHVQSQYQGWSKLDLKRYEEMRQSYGIHLPGLY
                     EILSNGFGFSFAMQHVLGLIRPAMQEIRTGQTAGRLAMLVERIRSFGPFHVRYGFHRP
                     EWIDELENTIDRAELNLEVLDQPWEWLRTAWGQVGISSRESAEILHEVSQWGHLVRNS
                     SMISFTLPEDDRSK"
     gene            12447..13277
                     /locus_tag="PLD05_09450"
     CDS             12447..13277
                     /locus_tag="PLD05_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOP77707.1"
                     /translation="MPIQITSLRNPRVKEAAALREARERRQRQEMIIDGVREIRRACE
                     AGIKLKEVFFCASLLTSEGKQLLSQVSESETELFEVTSRVFEKLNFGDRQDGIVAVAA
                     TPQRSLAEIEDQTGTALVAILEAIEKPGNLGAIIRTADGAGLSGVIAADLRTDLYNPN
                     TIRASLGTIFSMPVVAMDSRSAREWALARGLQLVAARVEGTIRYDQVDFTRPTGIVLG
                     NEATGLTPIWKRADVLGVRIPMLGQADSLNVSVAAAILFYEAQRQRGFPIPARRKRER
                     "
     gene            complement(13301..14278)
                     /locus_tag="PLD05_09455"
     CDS             complement(13301..14278)
                     /locus_tag="PLD05_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOP77708.1"
                     /translation="MSLTDTALPKAASELRLQPRKSSATVFGPYLCLAAALLYAGSNM
                     ALRRLAEPGQAADPIWVLFVKESITVLVVGPWLLCQSTRGRIRLPSQGNFLAIIAAAI
                     PTQVVANLGMLWAFGQVGIAIAVPVSLAVNLITAAILGRFFLSERVSPRTLLAMLLLI
                     VGISVIQSRADGLGNPVTVASPWQVVLAIGMCALAGLIYGWLSVVIRRVTTTGTSGWF
                     VLVLVTGVGVVTMGPMTLWNHGFQIVRQTAGEHWLWMIVSGLLNLIGFWALTKGLQMT
                     PVARANLLTSSQAAIAAVAGWLIFGEVLNLTVGLGLALTLCAIVLSSLK"
     gene            complement(14497..15654)
                     /locus_tag="PLD05_09460"
     CDS             complement(14497..15654)
                     /locus_tag="PLD05_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP77709.1"
                     /translation="MRCLQPRRSAGPVCQPPGLLTVVLCLWAGLASGSETGTRPTQLE
                     RVKVSADGRGFVLVPSGRSFVPWGFNYDHDAKGQLLEDYWHTDWDRVVGDFREIKALG
                     ANVVRIHLQVARFLEAPDRPNKENIERLKRLFQLAEELGLYLDVTGLGCYHRQDIPAW
                     YDALDEEARWAVQAFFWETIAQAGAGHPAVFCYDLMNEPVVPVDKRKAGDWLVDAALG
                     GKYFVQRITLDPRGRARKDIARAWVDRLATAIRKHDTDALITVGLLPGTKERADAWSG
                     FVPSELIPPLDFIATHIYPESEKLDVSLQILSHFSVGRPVVVEEVFPLRCNTQELKGF
                     LRASRQYASGWIGFYCGVPPDELRQSKELAAQITAAWLELFQELAPEFRQP"
     gene            complement(15777..>15970)
                     /locus_tag="PLD05_09465"
     CDS             complement(15777..>15970)
                     /locus_tag="PLD05_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77710.1"
                     /translation="ERVGMRASERRNRLRKPAAIRATLSPDPSPAERVRGDYGIGSNC
                     QSRPSPPDSQPECHHTPGL"
BASE COUNT         3696 a         4187 c         4583 g         3404 t
ORIGIN      
        1 gagtttgtct tcatcaaccg gagctgtggg gcagaacagc cgtttaatga gcaggaaccg
       61 gccgtggcgc aattgcaaca ggtcctcttc ccgttccccg gggcgatcaa tcggctcaac
      121 agttccacca tgaagttcac gccgctggtg aagacgggaa gaatcactgg tctggtcaat
      181 tatgatgaca ttttccgcat ggactttctg accggtagcc gggaactcaa cacgaatcgt
      241 cctttgcgtc cgaccaggac cgaatacgtg ctggcggcca ccattgaggg aaagctcccc
      301 gaaatgaaga tcacgaagtc ggaggaaagt aatcccgaac ccggcgctag cagcaacaaa
      361 gtggaggagc ttgcagataa acaacacgat gtccgggtta tccttgtggc ggacattgat
      421 atgttgcatg agacgttctt ccgtctgcgt caacgacagg atttgcccgg cttagacctc
      481 aagttgagct ttgataacgt cacgatggtg ctcaacctga ttgatcgggt gagtggagat
      541 gatcgcttca ttgatatccg taaccgacgc cccaagcacc gcaccctaac cacgatcgag
      601 cgggcgacgg aagcggcgcg tgctcgaacg gcagaagaac gccagaagct gcaagaggca
      661 tacaaccaaa tcgaaaaaga agagcgggaa aaactggaac agagtgtcaa gaagctcgaa
      721 gaggacatga aaaaacagaa tatcagtttg gacgaaattc tccgccgcgt tgctattgcc
      781 caggaacaag gtcaacgacg gctcgacgcc agaatggagg aagaacggca gcgtcgcgac
      841 cgggcactgg agcgaattga gaccgagttg accttgtacg tccggcgact ccagaacttc
      901 tataagatgg tctcggtaat cattccgccg ttgttcccgt tgggacttgc ggtgatcgtc
      961 tttgtgtggc gaagggcacg ggagctggaa ggtgtcccga cgtcccgccg tgtccgcaaa
     1021 aggttttgag aagtatcgtg ctgaactcgc gagaagccgt cagacccggc aaaacggcgg
     1081 gtggatgagg aaatcagatt aggataagca atcgtgttcg tggttgggcc caagatagac
     1141 gggaaatcag ccaatgccca tccgtgaaac gaggagatag acaacgatga acgaaaccaa
     1201 aaagacgttt gcctttgttg ccgtggccgc agtggttgtc ctcatcgcct ggtggacgcg
     1261 ctacaccccg cctgtcacga cgactgctga tatgcgaggg aagccgctct ttccgggctt
     1321 tacggacgcc ctggccgcca aaagcttgga aatctttgaa tatgatccca cgagcgtaaa
     1381 gatcaagaat tttaaggtgg ctcaaattaa taatcgctgg agcatcccta gtcatgagaa
     1441 ctatccggcg gatgccaagg atcacctggc acaagccgcc acaagcctga tcggcttgac
     1501 catcctggat gtcgccagtg aatcaccaac ccaggaggag gttgcactct atggggtcgt
     1561 tgagccggat gaaaagatta tcaagaccag tgtccgtggc atcggaaaaa gggtgatctt
     1621 tcgtgaccag ggtgacaaag tattggcgga cctaatcatc ggaaacaagg ttcctgacaa
     1681 agaagagctg cgatacgtcc gcatcaaagg acaagaccca atttatgtgg tgaaattgtc
     1741 cacggataag ttttctagcg aattcggcga ctggatcgaa aaggatcttt tgaaactcaa
     1801 tccctgggac atcaaggatg tgcagctcca tgattactcg tttgactcct taacacagac
     1861 gttggccccg cgatcgcaag tcatattggg ttatgacgat ctcggaagcc cgcgctggaa
     1921 gctcgtccaa aacctggtct tcgatggtga tcaggggacg tggaaaccgc agtcactggc
     1981 ggaggacgaa gagcttgaca caagcaagct ggactcgatg cgaacggcgt tggacgacct
     2041 gaagatcgtc gatgtccgcc gaaaacccga gggaatcagc gcatcgctga gtgccgacgg
     2101 aacgttggct gccaatcgcg agacggcggc gtcgctagcc caggccggat tctatcttgt
     2161 ttcggccagg gacttttaca agatcttccc catgatcggt aaaaaggagc tgaaacccaa
     2221 cgatgtggaa gtcatctcga gtgaaggcga gattcgggtg ggcatgaagg acggggttcg
     2281 gtatctcttg cgatttggtc aagttgttgc cggtggatcg tccggacagg agggggaagg
     2341 cggtgcaggc gtcaaccggt atctctttgt catggcagag tttgatccgg acttgatccc
     2401 caagcctgag ttacaaccgc tcccggaggt gccttccgag gggcaatccc cagcagccac
     2461 gacaggcaca tcccagagtc aatcggcggc cactgaagaa gcccaatcgg ggacatccgt
     2521 cacgcaaggc tcggcaccgc cggaacagtc ttcaggttca gaggaagcca aatcccaaac
     2581 atcagaagag aaaccggcag agcagcagaa aaaaccggaa gatatcaagg ccgagcggga
     2641 acggatcgaa aaggaaaaca agcgaaagca agatgaatac gaagaaaaac ttaagaaggg
     2701 gcaagagcgg gttaaggagc taaatgctcg atttgccgaa tggtactata tcatttccga
     2761 cgacgtctat cggaagattc acctggggat cggtgacatc gtgaagaaaa aggaaaagaa
     2821 ggaggaagag acgaagacat cggactcaag tactccgtcg cctactgagg aatcaaagaa
     2881 ggccgaggca acctcctcag gcgacacgtc tcaagcggcg aaagaatcgc cacaagagac
     2941 caaaccggtc gagtccacac catccccaga ggcaccggct ggtgcttctc cgcctgcacc
     3001 accgtcggag gaaacggcac ctgcgacggg tgctgcggaa caacagccac aaggcgggga
     3061 cagttcaccg gagcccatgc ctatgcagta atgctaagat tctgtctgcg tcgatcgcat
     3121 cacaaaaaag gcgttggcat cgccccaacg ccttttttgt tttttagggc ttgtatcccg
     3181 ctcatacata cccgcccccc gcaacttgac ggttgaccac caggtgccat aatagtgtat
     3241 atttgtacat gtcagcgggc cagttacatc aaagggggga atgccatggg accaaacgcc
     3301 gtcattgggg aatttgctgg tcaaattgcc gcagagttgg acgagttctg tcgttcgacg
     3361 gttgaacgcg tctgtgggac agaataccca gacgatatgt ttcggctagc cgaccggctg
     3421 ggcatggaaa ttatcgttga tggcggggag agccatcggg cgagatgtct gcgaatccaa
     3481 cgaggggaga aatcgcaatg gctcttgatt gtccgagacg aaccgcgctg ggaacgaatg
     3541 caatgggcca tcgcccacga gttaggagaa tcactcgctg gccaggccat catgaagttg
     3601 ggctgggacg ttgaggatct tcagcccgtc catcgagagt ggctggcgaa cgagatcgcc
     3661 aagcggctcc tcttgccctt tccggaattt ttgtccgagg gtgccgcgct tgaatgggac
     3721 ttgtaccggc tgaaagagca gtatcccaat gccagtcacg aagtcatcgc gcgccgcatg
     3781 ttggatgcgc cccggccaat tgtcatcacg atttttgaca atggcaggtt gtactggcga
     3841 aagagcaata gcctcatcgc atcaccgcct ctcacgcagc aggaaaaggc ctgttggcga
     3901 gctgcgcacg aaacgggaca gtccgttcgt tatcaaaacg ccgtggaatc gatcgtttgc
     3961 tggaccatcc acgagaacgg ttggcagcgg gaaattctgc ggacggaatc gccctggttg
     4021 gaggaatgac ccgccccggt gaagcgacgg tcatgcttct cgcgataaac tcggcaaact
     4081 gtggcggaaa tgagtttctt ttggattggg ggcgattatc atagaagata gcgatattcc
     4141 cagcatgggg gcgatcccgt tgcggaatgc cgcctatata ttctgacgcg gccgaaaagc
     4201 tgtagggcac ggatgctcgt ggtggtcatt atcatgctca gtgattcgag gcagaaccat
     4261 gacgctacga acaaaacaga cgatgtccgg cgttcgcagg gggcttgtcg gatggctttt
     4321 ccttgtcgct agtgggattt cggcttgggc aagcgccgcc gatttgattc atccccagcg
     4381 attaacacct tacggcttgg aagttgcttg gtttcagcag gtccaggttg atcctgctcg
     4441 gggacgtgtg acggaatggc ttctcgatcg aggaaccctg tttgtcgtca cgaacactgc
     4501 cgtgttacag gccttcgatg ccgagactgg gcgatccctc tgggctacgc cggtggggcg
     4561 acgcgagttg ctgaccctga aaccgacggc aggggagcat ctcgtcgcag tggtcaacgg
     4621 cagcacgatt tatgtactca accggtatac aggggtcatc ctttggagtc gccaaaccga
     4681 gggggcagtc ggagcggggc cggtcctcgc gccgacgcat gtttacgtgc cccttatccg
     4741 cggcaagatt gaagcgtacc gtttggagtg ggccaatccg gagacggccc tcgccgccag
     4801 gaccgcggaa ttccctcaag atcaggcaac atctccggcg accgcgggca tgacgaccca
     4861 atcttccgaa cagtctaacg gtgctgaatc cgtaacgccg atagctatga agcccgtggc
     4921 caaggaagag tttcgattat cgacccaacc acggcctggc catttcgtga tgtcatgggg
     4981 ccgggcactc gtcgagccaa ggctgacgtt tgcggaccgt aatcgggaac ggttggcgtg
     5041 gcctacggac aaaggtttgc tctttgtggg cgaactggat gtgcgctccg agttgcggtt
     5101 ttctgtgcag tatttctttc aaattagcgg cggtgatatt gtggcgccac catgtattcg
     5161 tccaggtgac ccttccaatc cggcagacat ccagcgaatg ctctatgtgg ccgctacaaa
     5221 cggattcgtt tatgccttgc gggaatggga cggcaaactg ctgtggcggt tttcctcagg
     5281 tgaacccatc aaggagtcac ctgctctgat cggcgacgac ctgttcgtca cgacccagac
     5341 gggtggaatg tatcgcctca atgcggtttc tggtgatctg atttggtttg ccagggatct
     5401 ccgacgtttt gtctcggcca gcgatcaacg tgtatacgcc gtggacggtc tcaacaacct
     5461 tgtcattgtc gatcttcaaa caggttcgag ggtcgggcag ttggatgtgt cacgctatcc
     5521 gctgcgatat gcgaactccg aaacggatcg gatttacctg gccagcgaca ctggcctgat
     5581 ccagtgtatt cgcgaggctc gcttgccgca gccgatcaag cacgtggtgc cgatcactcc
     5641 gcttaaggaa cagaaacccg caaaaccagc tgcagcgcaa ccggccgaaa ctcccgcaca
     5701 gcctcaaccg cctccgcaac ccgcggggga aaatcctttt caatgatgag gcgtcatcat
     5761 tgatccgcct actatctctg ggttcttgct cgccaacggg tttcgttcac ccgaaagcga
     5821 catatttttt gtgcgcggag caaacttggt aggaagtggg agtggcgtaa aaaaagcgac
     5881 cgagcctggg ccttgcgtcc aagggcgaat cgggccacgg cttcacgcgg caagggattt
     5941 agaacctatc ccaaaaagta ccccctctcc ctccgggaga gggttggggt gagggcaagc
     6001 gatcgtcggc gtcaatcgag aaaagccgca gcgattcgag ccacccctaa cccccgaccc
     6061 ctctgccgcc gaacgggcga gaggagatga tgggataggt ccttatttgc gggtatcagc
     6121 gggcgtgtat tctgtaacat tgagatcgct gatttgaacc gaacgaaccg tatccgctcc
     6181 gaggattcgg ttgacccgtg cggtgatctt gcgactgaga aaatccgggt caagtacttc
     6241 tgaaagcgga ctggcgcgta ccgtgacgag aatttcatca cggataacat aaccggtacg
     6301 tctgataaag ttctcaaact cgtgttggtc agaaaacgcc atcacggcat ggagacgaaa
     6361 acacgtgctc cggacaagtc ccgacgccgg gtcgtaagtg taaaccgcaa tttgtccaaa
     6421 gtcggcaacc ccgtctcctt gccaaacagc ggcaggcgga acgtggcttg tttcgtttcc
     6481 ccagctttca cggacgccta tcttttccag ccaattggtc caaagactct tggaacctgc
     6541 cacggctcca atgaggaaaa ccggggcgag ggcgataagc aagacgagtc cctcacccac
     6601 gtgagaaagc gagaacacac gccgtttctt gaccaccggc ggtttgtcat caccctttgc
     6661 tggcggcctt gccgtgggct ttccttctct ccgagaaata tccaaaggag aacctcgcaa
     6721 tagaaactgg ccggaccgag ataagggaag agtgaatcta tcccaatcct agctcacaaa
     6781 ctcggatcac gctccggcaa ttcggtgcat ccggttcgaa acaattgaac cattgatgcg
     6841 tcaaggagtg agactaacac tcgtatccgg caggacgctc gtaaagcttc ttccgcgttg
     6901 cccataaacc ggccgacggc gtaggaatga agtagatggt ctccccatcg ggggtggtgt
     6961 tccagccctc gcgcattttg tcgggcggat atcgacgcag ggtctcctgg aggtcagcat
     7021 agccaaagtt gacgccctcg atctcttctt tggtgaggtg cccgggcgcg taggtgattc
     7081 gaaatctccc ctcggaagaa ccatgaataa gatgggccgt ggcgtgggcc agctcttgga
     7141 ggtcttgatt ttgacgatac agctccataa cacgctgagt tcccacatat ccgtacttgc
     7201 gaatgacccg atcgacttcc tcctgttcgc cgaatcgctt caatcccggt gcgatgatga
     7261 taagctcgcc gccatcggcc atcgccatgc gggtacgata gatcgcctta ttggcaaccc
     7321 acgtgctaac gaactcatct tcctgcatga cgcacacgac tttctggaga ggctcctcga
     7381 agaccttgat gttctcctca cgcgattgcc gggctgccat aagataggtt tccaagtcgt
     7441 cacccacgta gatgcccgta tgcacgagcc gatcttccgg atcgcgggcc atcacaatct
     7501 ggatataaaa atctggaaga gaaccgagga actcgttttc cgccttgtta taacaagcac
     7561 gaaccggcgt gatcagcgtg ccgaggttat tctcaatacc acaacaagcc gcggcgatgt
     7621 gcgaggcgca gatggtccct tttccagcca aaccgatgaa gtaattcttg ttgtgattgg
     7681 cgaaccccaa cacctcatgc ggtacgacat gtccgatatt gatcaccaag tcccagggtt
     7741 cttgcatgag catgctattc agttcaatcg gaatggccca gttggccgcc ccgtgggtga
     7801 cctgttcaac gaaattggct ggaacttcgc caaggcggac gaccgtgtta atccagtggt
     7861 gggcgtggat tctctcatgg ggaatggaac cgaacatccg cgcgttttgt tcccggctgt
     7921 gcggctcatg ctgaccgagc gtgggaatga catgnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnntccatg agtgttcccg aagaccacgg agtttcagcc aagctctcgc
     8101 tcaggttatc caaaccgccg ccgtgcggta aaatgcggga ataggttgag tggccgcgtg
     8161 tgcgccgccg acgctgcttt gggtgagagg gcacgttgtg cccgtggctt ggatacgctg
     8221 tgcacgtgtt agcaggccat ggaaaaagcg gttcaggggg cctgaaatga acttgagcgc
     8281 tgagtgaaat gccacaccct agtcgggctg gatgctcgac ttgagatggg tggatggatt
     8341 taggctggga aggattatca aggagctggt gttgaatgaa gccagaaaag tcagcctctc
     8401 cggtagaggt gattctcggt tacctgaatt tctctcaggg ggtccgagat ccgcggttct
     8461 ttgctgcgtg gaatcgcctc tggacactcc gggaaattgc gttcctttcg caaccctcag
     8521 aggctcaaac tcaagagagg ccggagttgc tgcatcggta tcttgataac gaactgagcc
     8581 gactgcggcg cgaaaatcct gctttttccg atacggccca ggcagagtat gtcctagaat
     8641 ttctctttga acgcctgatt cacggctacc ggcagtttca ccgcgatatc ttggctcatc
     8701 ttaccctcga cgactattgc caaccttttc tgctcgcacg gttttgtgaa gccatcttag
     8761 cggagctgca cgaggccgag cttgacggaa aggccgatga taacgagatg cttgaagcca
     8821 ccatcaggcg gctgaatgac tatttgggct atcggccggt gcccacgctc gagggaaaaa
     8881 aacgtcacaa accatacgag cacgagtttg tctgtccgat tcccctttgg atccgtggcg
     8941 caggggccgc cgtaggcccg tatcaacgat tgattgagac agcgctggga attctccgcg
     9001 agacatcggc ggagctttgc cgggcgagtt attttgactt cgagcaactg gaggaaatcg
     9061 tcctcgatcc ccgcggttat gatatggaac atcccgtgca ccagcggccc aattacctct
     9121 atgggctatg ggatccgcgg aaactcaatg accgtggcta ctactgtcgg tttgtccttc
     9181 atgatgttca tttgaacagc atcctcgccc gcgtgttgga aggacctgcg gacgattatg
     9241 aggagcgcct cttcgaaggg gcagcagtcc tggcagggac aattctgatg gcttcgggaa
     9301 tctccggaga ccgcccgggg gccattctct ccagtgtcca gatcgacaaa tggcttcagg
     9361 gaattgccct tgtgcgggat cagttttata tcgagcttct cgggcgatgt actggagaga
     9421 gacggcagcg gctggagtct gagatgcaac gcctgcgtca accatttggg gccacccgcc
     9481 aggcgctcaa tcaaaaactt gcggctcgtc gctctcaaga gttgtacgtc gtctcgttgg
     9541 ccacctgctt tgccaagatg ggggctgtcg aagaggcccg ttactggatg caacaaatcc
     9601 attcgggcgc ggcacggatt cgctgcgaga ttcgttgtca gcttggcgag tgtgaatggt
     9661 tgattcgcaa caaccggcga acagaggccc tcaagacgct ctccggtgtc attgagctga
     9721 ttcaccgcgg gattgaatgc ggggccttaa tcgacccctg gtgcattctg ggatttgggt
     9781 gcctctttcc cattttcgag catttgcggg agggcgtctt tgactaccgg gtcgagcaac
     9841 tcgtcggtct ggttcgtctt gttttccagc gattggcggc cttgcgccgg gctgcggctg
     9901 cttcaggcga cgaagccgtt gatgaggccg ccgaaaacct tgctgctgag cttgccgatt
     9961 ggtgggacca gtttgccact acggcggtgg ccgacgccaa tagctttctg ggacgcgatg
    10021 aatgcactgc cgctacagtg gtcagtcgta ttttgaagcg atggcgagaa gagccggagg
    10081 ccgcccaaag cctagcgttt tggaaagagc acgccggctc gttgcagtca gccgccagtt
    10141 atgtcttgct tgtcgagacg ctcctgcagc atcgggatac gacggcagcg atggcgctct
    10201 tggtagattg ggcaagcagg gcggacgaag tgggattggg agtcggcgaa caatcgtttc
    10261 aggtcatggc cctgcggtgg tttcagacga tttggacaga acaaaaggac attaaagatc
    10321 gctggaagtg gacccaaaag tttatcgact acttggaggc caattgcgag tccctgtttc
    10381 gtgtgccaaa tctctcagag tttttcaagc gggccggaag ccctgtcatt cgttccgagg
    10441 acgaggaaaa cggtgatgac gatgtgttga gtgcggctta tgaaggagtg atctttcgcg
    10501 acagtgctgc cgatgggcac gagggcgaca cactggatga cgagatttct gattccgaac
    10561 tggcgccgct attgggtgaa atttctgatc gctgcaattt cttaagtgcc acgaccttga
    10621 ttcgatacga tgccctattg gcttcgttcc gtgccatttc ggaagtgcct gactcagcga
    10681 caatcctgat gacgtggtac agggacgccc aggaagctga ggaacggttg ttgcaactgg
    10741 cagaagagat ttggcgatat accctcgtgc cgccgtctcc gagtccggaa ggcatgatgg
    10801 agtttgagcg gcagcgccaa attcgtgatg cgctggtgga tcgcgtcatt caaacagcga
    10861 taaacgtcct tagcttgcga tatttgttac gggccattat caacgttgag gcaacagagg
    10921 atgagtccca acagccacag tgggatacct attatccgcg gcttttgcgg gctgttttgt
    10981 acggagacga ggccgccttt catcaattga tggaatcttt tgccaaggtg atcgttaccc
    11041 gtcctattct ttatttctcg cttgtccgtg gtggaaatcc gcggacatac tttttggcgc
    11101 gtggcacgca ccgggccttg ggtttgctgg cctatcttat cgcccgtgcg ggatgggtca
    11161 atgcggccat ggggcttttg agtctggtga aaagtgccga gcgtcaccag tccgaaggca
    11221 agggcaaagt ttctgagatt gagcatttat ttgaggccgt cgccgaaggg attttcttgg
    11281 ccttggtttt tgctaagcga aatggagcca aaagaaaaga gggccgcctg tcgcgacagg
    11341 aaaccgagga cctggtggct gacttgcatg cagtttgcaa cgggctgctc gagatttggg
    11401 ccgtcattat tcacgagatt tacttctcca cggtggagac ggctcctttt cggcggtctt
    11461 ggacgcaaat ccgccaattt attcgcgcct ttgggcagga aatcttcacc ccgatgttta
    11521 tggcccatcc taacctccgc gctattatgg cgacgggaac gcatcactgg ttgaagatgg
    11581 tcaaagagtc gggtgacgcc caatggccac gcttggtgga ggccttgcga tccggggaat
    11641 tgaagctcaa aaaggccgcc gagtacctac gtttgatcat cgatgtcatt ctcgagaact
    11701 acgaggacta tttagattac aacagcatga cgactcagtc cgattacggg cagaatctct
    11761 atatcctgct cgatttcctc cggctgaagt gcgaacatca aagacgagcg tggatgctca
    11821 agccgtttct tctggcccat cgcgtcctgg taaggctgga actgtgggag gcagccgaga
    11881 tttttgagga acacgtgcaa tcacagtacc aaggttggtc gaagctcgac ctaaaaagat
    11941 atgaggaaat gcgacagtcg tacggtatcc acctcccagg cctgtacgaa atcttaagca
    12001 acggtttcgg tttttccttt gcgatgcaac atgtgttggg cctgattcgg ccagccatgc
    12061 aagagatcag aacgggccaa actgcggggc ggctcgccat gcttgtggaa aggattcgct
    12121 ccttcgggcc cttccatgtc cgatatggct tccatcgccc ggaatggatc gacgagctag
    12181 aaaataccat cgaccgggct gagttgaatt tggaggttct cgatcagcca tgggaatggt
    12241 tacggacagc ttgggggcag gtggggattt cctctcgaga aagtgccgaa atcctccacg
    12301 aggtctcgca atggggccat ctcgtgcgga atagttctat gatttcattc actctccccg
    12361 aagatgatcg ctcgaagtaa gcatcagtca taaggttgcc gtgaaaacca aggtgtacca
    12421 tcgattcacc gagatatcgg gaacctatgc cgatccagat cactagtctt cgaaatcccc
    12481 gtgtcaagga ggcagcggct ctgcgagagg cccgcgaaag acgccagcgc caggagatga
    12541 ttattgacgg cgtccgggag attcgtcggg cctgtgaggc aggtattaaa ttgaaagagg
    12601 tctttttctg cgcctcgttg ttaacctcgg aagggaagca actcttgtct caggttagcg
    12661 aatcggagac agaattgttc gaggttacct cccgagtatt tgaaaaactc aattttgggg
    12721 atcgccaaga cggtattgtt gccgtggctg ccaccccgca acggagcctg gccgaaattg
    12781 aggaccaaac tggtacagcc ctagtcgcga ttttggaagc catcgaaaag ccgggcaacc
    12841 tgggggccat cattcgtacg gcggatggtg cgggccttag cggagtgatt gcggccgatc
    12901 tgcgaaccga cttgtacaat cccaacacca ttcgcgccag cctggggaca atcttctcca
    12961 tgccggtcgt tgccatggac agccgcagtg cacgggaatg ggcattggcg cggggccttc
    13021 agttagtggc ggcacgtgtt gagggtacca ttcggtacga ccaggtcgat ttcacacgtc
    13081 caacagggat tgtgctaggg aatgaggcca ccgggctgac accgatctgg aagagagcag
    13141 atgtccttgg cgtacggatt cccatgttag gacaggccga tagtttgaac gtctcggtgg
    13201 ccgcagcgat tctcttttac gaggctcaac gacagcgcgg ttttcccatc ccggcacggc
    13261 ggaagcgcga acgttgaact gaccactgga ggtgggacgt tcacttgaga ctactcagga
    13321 cgatggcgca gagcgtcagg gcgaggccca agccgacagt gagattgagg acttcaccga
    13381 aaatcaacca acctgccacg gcggcgatag cggcttgaga ggatgtgagg agattggccc
    13441 gggcaacggg cgtcatctgt aaacctttgg tcaatgccca gaacccgatc aagttgagaa
    13501 gtcccgagac gatcatccac agccaatgtt caccggccgt ctgacgcacg atctgaaagc
    13561 cgtgattcca gagggtcatg gggcccatcg tcacaacacc cacgccggtt accagaacta
    13621 ggacaaacca gccggaggta ccggtggtgg tcacgcggcg aattacgacg ctcaaccagc
    13681 catagataag cccagctagg gcgcacatcc cgatggccaa cacgacctgc cagggcgaag
    13741 cgaccgtcac agggtttccc agtccatcgg ctctggattg aatcacgctg atcccgacta
    13801 tgaggagcag catggcaaga agtgtccgag gggatacgcg ttcggagaga aagaatcgac
    13861 cgaggatggc agcggtaatg aggttaacag ccaaagaaac cggtacggcg atcgcgatgc
    13921 cgacctggcc gaacgcccag agcattccca gattggcaac aacttgagtg ggaatggccg
    13981 cggcaatgat agcgaggaaa tttccttggg aaggtaggcg gattcgtcca cgcgtggatt
    14041 gacagagaag ccacggtccc accacaagta cagtgatgga ttctttcaca aagaggaccc
    14101 agatgggatc agcggcctgg cccggctctg ccaaccgccg cagagccata tttgatcctg
    14161 catagagcaa tgccgccgca agacataaat atgggccaaa gacggttgct gaagacttgc
    14221 gtggctgtaa ccgcaattcc gatgcggcct ttggcaaggc ggtatcagtc agtgacatgc
    14281 ggcgggatcc tacaccatta aatcttcaaa aacatcattg tagcaaagaa acaattgtca
    14341 ggtgtggcta cctaaaaaag tagtgagatt ttgtttattc tatctggaag tctcgcaaac
    14401 atttcaactg atatcgtaaa gactcatagg ctggcagtgg acgagcctct gttaaaatca
    14461 acggcgagag attgcccatt gcggcaacca gcgtgatcac ggctggcgaa attccggcgc
    14521 taattcttga aaaagttcca accaagctgc cgtgatctgg gcggcaagtt ctttggattg
    14581 gcgcaactca tcgggcggga cgccgcagta aaagccgatc caacctgagg catattgacg
    14641 cgaggcacgg aggaatcctt tcaattcctg cgtattgcat cgcaaaggaa aaacttcttc
    14701 cacgaccacc ggtcgcccaa cggaaaagtg ggataagatt tgtaatgaga cgtcgagctt
    14761 ctcgctttct gggtagatgt gagtcgcgat aaagtccaac gggggaatca attcactcgg
    14821 tacaaagccc gaccaagcgt ccgcccgctc ctttgtcccc ggcaaaagcc ctaccgtgat
    14881 gagcgcgtcg gtgtcgtgct tgcgaatggc cgtggctagg cgatcgaccc acgcgcgggc
    14941 gatgtctttg cgggctcgtc cccgagggtc aagcgtgatc ctctgcacaa aatatttgcc
    15001 gcctagggca gcatccacca gccaatcacc tgccttgcgt ttatccaccg gaaccacagg
    15061 ctcgttcatc aggtcgtagc aaaagaccgc ggggtggccc gccccggctt gggcgatggt
    15121 ctcccagaaa aaggcctgga ccgcccagcg cgcctcttca tccaaggcat cgtaccaagc
    15181 gggaatgtcc tgccggtgat aacagcccag gccagtgaca tctagataca gcccgagctc
    15241 ctcggccaat tgaaaaagtc gcttaagcct ttcaatattc tctttgttcg gacggtcggg
    15301 agcttccaga aatcttgcca cctgaagatg gatacgaacg acgttagccc ccaaggcctt
    15361 gatctcccga aaatcaccga ccacccgatc ccaatcggta tgccaatagt cctcaagaag
    15421 ctggcctttg gcatcgtgat cgtaattaaa gccccagggg acgaaacttc gtcccgacgg
    15481 gacgagcaca aacccgcggc cgtccgcaga gactttcacc cgttcgagtt gtgtcggccg
    15541 agttcccgtt tcagaaccgc ttgccagccc ggcccagaga cataatacaa ccgtcagcag
    15601 tcccggcggc tggcaaacgg gacctgcgga acgtcggggc tgcaaacacc tcataccgat
    15661 ctccgctgga aaacaacacc ttcatccaaa gcagtaaata ccgtactgcc gtggtccgca
    15721 caatcttctt ttccttcaat gcaacaaatt gcctagatta cgaagtctcc aggctgtcaa
    15781 aggccgggcg tgtggtggca ttcaggctgc gaatcgggcg gactcggccg cgattggcag
    15841 ttagagccta tcccataatc tcccctcacg cgttcggcgg gagagggatc ggggctgagg
    15901 gtagctcgaa tcgctgccgg ttttctcaat cgattccgac gttcgcttgc cctcatccca
    15961 accctctccc
//
LOCUS       DAOSUT010000089        15902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2267_length_15902_cov_10.724049, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000089 DAOSUT010000000
VERSION     DAOSUT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15902
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_15902_cov_10.724049"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1340
                     /locus_tag="PLD05_09470"
     CDS             219..1340
                     /locus_tag="PLD05_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOP77711.1"
                     /translation="MSATRLILVGGFLGAGKTTLLTEAATRLIRRGKRVGLLANDQAP
                     DLVDTEVFKTSGAGVEEVAGGCFCCRFPDMITAMERLLQHSRSDVILAEPVGSCTDLS
                     ATVLQPLKDRYRDRFEVAPFSVLIDTTQVCALARLPATTVPAAPPRFPDKVLYIYEKQ
                     LEEADAIVLNKVDLLSPEEVSGLKQALATRFPEVPVFTMSALTGDGVDAWLDFVTQSQ
                     AAGRRIAQVDYDTYAAGEAALGWMNASARLRASTPVDWRTFAADTLDAIRTRLRNASG
                     EIAHLKIFLTTPGAYVAGNITSNEGPVSVRGEIDAARSTVSLLINARVHAHPDILRAI
                     VEEGLRATAGDRVEVTITNIRSFFPGRPEPTYRYRNTVQ"
     gene            1511..2632
                     /locus_tag="PLD05_09475"
     CDS             1511..2632
                     /locus_tag="PLD05_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOP77712.1"
                     /translation="MLTRREMLGVTTGTLTGLGLFSGVARAGSNSLEPVREEGIAWYD
                     VTQWGVEGRGWNDTARFFDRLPARAEKVVRPAVWNLSRHSAGMCARFVTDATLIRVRY
                     SLLSANLAMPHMPATGVSGLDLYAQDDRGVWRWVAVAQPTKQEMDLTLVSGVRPGKRP
                     FMLYLPLYNGVESLQIGVPENADFQPVPPRTDGLIVYYGTSIAHGACASRPGMAFPAI
                     LGRRVNRPVINLGFSGNGRMDLELVPFLGELDAAVFCIDCLPNMQADEIKERAEPFVR
                     SLRERKPQVPIVLIEDRTYTNAWILPDREKRHQTSREALKAAYQRLLAEGAKELYYVE
                     GEHQLGDDHEACTDGSHPSDLGMMRMADVLEPVLRPLLK"
     gene            2835..3371
                     /locus_tag="PLD05_09480"
     CDS             2835..3371
                     /locus_tag="PLD05_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77713.1"
                     /translation="MLGFVHVMIEPTGDKFFAGLLVVDHFGDPHELRVTKAVQTTVVQ
                     KLLWGQRLLGHVLHKVILVPLAGQLGSQVEVIFVDRAEFLSGRQDLSVPLILLESRAD
                     IEESAQSETSVSVPDSKGNGVLLLSCAPGFETDLEEGRRLVTEFSKTCYPLEPFQRVE
                     HILKHLKPDLETRTGAKG"
     gene            3524..5440
                     /locus_tag="PLD05_09485"
     CDS             3524..5440
                     /locus_tag="PLD05_09485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOP77714.1"
                     /translation="MARSSSRLEGNWWTIDVAAFPPAVVISTKYESLKSEVRNVAIGR
                     HRPSPAMVFPVECRCPEPQALDVATEPDRPAIEKVSDLDFAPPQLDLIPVSPVSAEIV
                     EIPTESFDIPLIIGRVRRSGSSSEEKASQPLRPTSGKLGDLAPKPIIPPALWEQIWKI
                     LSPPPRIEEIVSEKYPGQLRPYQVDGVRQLIEKERFILADEMGTGKTVQVCVALSLLV
                     RLGRIRRILIVCPKTVLTVWTTHLRDWTPEIVVHSFDDFYSPRVAVNDTRAHAWVTSY
                     PRMGMQADAVIYWGTKWDVVVLDEVHEIRNSNTKKYRNLQIITSRARYRWGLSGTPLQ
                     NRLEELTNIFQIVVPELKLRAETLQLNQVREKIRPWMRRVTRKAALPDLPPKERVEIW
                     LKLDPEQVREYEFFAGNAARQMPIGAAQMFTHIWQVLMHLKRICNFASNSHSSPKTQK
                     LVELVEQITRRGEKVVVFTQFRHYGVDRIAPHLRRFGLAVIHGGCSDAERFSAVQQFQ
                     NNPQCRVLLGTVKTTGFGLTLTAANHVIHFDHWWNPAVAWQAEDRVYRIGQTRPVTVY
                     EFWMKGTVEERVRQILEEKGLLHQEVIERLSDAEFKRLFTLDELLALLHLRPSSLDAH
                     GAASRSQQTGTTTL"
     gene            5830..7794
                     /locus_tag="PLD05_09490"
     CDS             5830..7794
                     /locus_tag="PLD05_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP77715.1"
                     /translation="MHVFKSLLTSMVVVSVISSANGEGLSQPGNPSPSPNAVRAGRFH
                     VEPPTLLNLGFEWAIEGDANRNATVDVRFRQVGEIEWRQALPLVRIGGERVFREREGL
                     DYTVPDGFAGSILNLQPGTEYECQFLMRDPDGVEGQAVHTVRVKTRSEPQPFPGGRVL
                     HVYPPDYQGERQEPSFSSIMQAYYGAGLGDWNVVWEHRVQPGDTILVHAGLYKPDRRN
                     YVDPLCTPFDGTTYLTLKATPDRPITIKAAGDGEVIFDGGGNHRLFDVMASAYHIFEG
                     LTFRNTDVAIFAGQKGVTGAVGLTVKNCRFENVGFGVWTESPYSREFYIADNLFIGRE
                     DRFRLIGWTGPLWPSAGRYGSHELVSYYAIKVYGPGHVITHNAIAYFHDGIGISTYGT
                     PENDPDLRASSIDIYNNDIHMTNDDFIETDGAVHNVRVFRNRGVNAAQGGYSAQPSFG
                     GPVYFYRNILYHVPTGVSFKFSANPAGLFVFHNTIIGEQVGMGPSSNVHWRNNLFVGT
                     DAPGRAIMSWNNATESYSSDYNGYRPNRGVSAQYRWRVPTAAQPQVFATLAEFRSATA
                     QEKHGIEVDYDVFENLRPPDPSNRHAVYHAMDLNFRLNPHGAAVDAGVIIPTVNEDFT
                     GNGPDLGALELGQSEPHYGPRWLTWQPFYR"
     gene            complement(8241..>8555)
                     /locus_tag="PLD05_09495"
     CDS             complement(8241..>8555)
                     /locus_tag="PLD05_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77716.1"
                     /translation="GRIRGEYGGFNQKFIGDETGKKRYEEALAQGLSLKKPGLPPNVR
                     PGGHGGSHGYLAHDFIDAILRNRKPAVDIIDALHMTVPGYYAHLSALKDGETMRIPTY
                     SL"
     assembly_gap    8556..8655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8656..9251)
                     /locus_tag="PLD05_09500"
     CDS             complement(<8656..9251)
                     /locus_tag="PLD05_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77717.1"
                     /translation="MSKISRRTWLMGSALAASQGVFAGGVSGEPIPGFEQVQVETDPN
                     KPWEPYTDRKVRLGIVGHGYCKFGLAFDLQYHPNVELVAVSDLIPERCAEMAKLAKCE
                     KTYPSLEEMVKDDSIEAIFCATDAPSHARHAILCLEHGKHVATAVPAFYGDIEDAERL
                     LECCKKNRGLIYAMFETSVFHDDLYAMEKLYAANVFGH"
     gene            complement(9449..10816)
                     /locus_tag="PLD05_09505"
     CDS             complement(9449..10816)
                     /locus_tag="PLD05_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77718.1"
                     /translation="MRRLRFSRRRMLAAASALAVPYWFTHSRVMGQGGEEKNDRPRIA
                     AIGVGGRGTAILKSAARFGDVVAVCDADLQHAEAASQLFDGKPAVYQDYRKLLEERQD
                     IDVVLNGTPDHWHTAINVMACRLGKDVYTEKPLGLTIEEGKIFRQVVAETRRIVQVGT
                     QQRSVAHFRLACELVRNGRIGKLQRVIVTLPFWNTKGGPFKPQSVPPHLNWDLWQGQA
                     PERPYYPERVHFNFRWWSEYAGGIITDWGQHHMDIAYWGMDMENSGPLWVEGEAIFPN
                     EGKPDCYDNPDRFFVKMLFPGDIELLYFVAHDERYRATITSEEDVQLFNQAQLDLPET
                     SRNGIMFIGEKGRIFVNRGGAYGKAVEELKENPLPANAIRLYESDDHMANFFDCVKSR
                     KQPASTVAVAHRVLTACHVANIAIRLKRRIYWDATKEEIIGDAEAAQSIYVKRPQRPP
                     YTIEV"
     gene            complement(11193..13454)
                     /locus_tag="PLD05_09510"
     CDS             complement(11193..13454)
                     /locus_tag="PLD05_09510"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOP77719.1"
                     /translation="MRRFEAIAFLVAGVVAVTVNAQRGVALDVIRNGRPVAVVVVETA
                     DRPSNVGRLRGDTWDDRRAAQVLVEWVEKMTGARLTVVGSVPPDQPAIVIGSVAIKAG
                     LKLDDIDSPSQEGLRISLRGDRLLLAGQSGIATTRAVCRLLESWGCRYFVDHPLGEVY
                     PKVNTLTIERLEVTEKPSFVYRSIWGSQWSGQTLWKVWNGAGGLPLQTQHAWGRYISK
                     DLFETHPEYFALRAGQRRAGDWYCTSNPEVRHLFAQGVIAEIAKGTRHPSISPPDGRG
                     YCECAACLAQDDPHLIEPSSGRVSVSTRYVDFFQDVARQVRQAAPDSILNFYCYADYT
                     QAPSRVTKLEPNLCAWLAPIRYCRLHRIGHPNCPSRMQLAQLVEAWAAIAKNLGYRTY
                     NYNLAECLVPFSMISIWKHDIPYLKSKNCLGINLETLANWQIYGPHIYLSIRLAYDPQ
                     ADADAIMDDYFEKFYGPRAGPLMKQYWLDIDRAFVDLNCHAGGFYAVHLVYTPDFLKH
                     CRDLLERAAAAAEGEPACLARVRMAAEGLENAAQYETIRQAMNRGDFVTALQTYENLL
                     ARSKKNVEEGWGNHYTVAYLERFLGPQVRAGAAVTASPNRLISVLPDKWRMTYDPDDQ
                     GVALGYNKKDYDDSGWHEVLTYSKPLDAQGLPDRQTILWYRTRFSVPKTAKRLSLFFT
                     EVDGDATVFVNGQEVGTSPKKRVPFEVDITSAITSGENVVAVRVDHSQITELFLGGIL
                     RPVLLVERAAEKK"
     gene            13775..15745
                     /locus_tag="PLD05_09515"
     CDS             13775..15745
                     /locus_tag="PLD05_09515"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOP77720.1"
                     /translation="MRFRQIPLSLMVLIVLAYIAALQGQGVAGQSVAEEPPSAPKYDT
                     YVKAPPPQEFLQRLRKTHPRLILLDEELPKIKAFLEQDVRGKAWYRNARERAEELYAR
                     PVVKHELPDGRRLLSISRDVLDRMYHWCFFYRMEKDKKWLDRAWREMEVVAEFPDWNP
                     SHYLDTAEMMHALAIGYDWLYQDLTEAQRKVIREALWRHGLRLSYSAYKGLKAEGQQS
                     WTTVTNNWNFVCNGGSSIAAMALLDEMPDECSEVLYHAFQRIQIPLQGFEPDGAWWEG
                     LGYWGYSMRYFCPYLRALETAFGTDFGFIEALKNTGFAKAGDFPVYLVSPFGTNFNFA
                     DSGSDTSHFSHWALFYLADKFENPLYQSFQEERTSGGLQDILYFRDLGKSGNLAAMPL
                     DKHFRGAELASMRSSWTDPNALFVAIKCGKNGISHAHQDLGSFIFYALGERWFIDPGT
                     ESQTYLSHQHHLPRSHFYRIREEGHNTLVFNPDEGFSQSGSGTSKIIRMVSSADEAFA
                     IADLTDAYRRHGVSVKRGYRLTQGRSVFLVQDEIESDHDNEVWWFAHSAAGTKFSLTD
                     DGRHATLERSGKTCHVYLLAPSDAVFQVMDAKPLPSSPNPSIQNPNQDMKKLAIHLPK
                     VQNVTLAVLFVPVPSGQRPSVTTLPIRPLENW"
BASE COUNT         3477 a         4388 c         4352 g         3585 t
ORIGIN      
        1 cccaaaaagt ccccctctcc ctctgggaaa gggttggggt gagggcaagc gatcgccgga
       61 gtcgatcgag aaaaccggca gcgattcgag ccaccctcac ccccgccccc tctcccgccg
      121 aacgggagag gggagattat gggataggct cttattattg acccagaatt tggcttgtat
      181 ttgatggtga ggtcctcgtc ccgttggaga gtgatcttat gtcggcaaca cgattgattt
      241 tagttggtgg ttttctgggg gcggggaaaa cgaccctttt gacagaggcg gccacacgct
      301 tgatccgtcg aggcaagcgt gtcgggctcc tggccaatga tcaggcgccc gatctcgttg
      361 acaccgaagt tttcaagacg agcggtgcag gtgtggaaga ggtagctggc ggctgttttt
      421 gctgccgatt tcccgacatg atcaccgcca tggagcgatt actccaacac tcgcgcagcg
      481 atgtcatcct cgccgagccg gtcggtagct gcactgacct atcggccact gttctccagc
      541 cccttaagga ccgctatcgc gatcggtttg aggttgcgcc attttccgtg ctcatcgaca
      601 ccacgcaagt ttgtgcgctg gcacggctac cggcaaccac cgtgcctgcc gccccgccac
      661 gattcccgga caaggttctg tacatttacg agaagcaact cgaggaggcc gatgcgattg
      721 tgctaaacaa ggttgacctc ctctcgccgg aggaggtttc tgggctgaag caggccctgg
      781 ccacccgctt cccagaggtc ccggtgttca caatgtcggc gctcaccggc gacggtgtgg
      841 acgcttggct cgatttcgtc acccaaagtc aggccgcagg ccgaaggatt gcccaagttg
      901 attacgacac ctatgcggcg ggggaagcag ccctcggatg gatgaatgcc tcggcgagac
      961 ttcgtgcttc cacgcctgtc gattggcgaa cgttcgccgc ggacacactc gacgccatcc
     1021 gcacgcgact tcgcaacgcc tccggcgaga ttgcccacct taagattttc ctcaccaccc
     1081 cgggtgctta tgtggcgggc aatatcacaa gtaatgaggg gcccgtatcc gtccgggggg
     1141 agatcgacgc cgcgcggagt accgtttccc tccttatcaa tgcccgggtc cacgcgcatc
     1201 ccgacatctt gcgggccatt gtcgaagaag gcctgcgggc gaccgccggg gatcgcgtcg
     1261 aggtgacgat caccaacatc cgaagttttt tcccaggccg tccggagccc acgtatcgtt
     1321 accgtaacac ggtgcaatag cggcatcttt tttatgctgc ttgggcaaaa cgtcacccca
     1381 gtcaatcgaa gacagcctga agtgtcgtga gcctggccag gagttccctg gtattttcag
     1441 ccgttggctc ggttgatcga aataaaggcg gaattgatgg ccccacaagt cttttgctaa
     1501 ggaatccgcc atgttgacac gccgcgaaat gctaggtgta accacgggaa cgctgaccgg
     1561 gttgggtttg ttttcaggag tggcgcgggc tggttcgaac tcactcgagc cggtgcggga
     1621 agaaggcatt gcgtggtacg atgtgaccca atggggtgtg gaaggtcgtg gctggaacga
     1681 tacggctcgc ttttttgacc gcctccccgc ccgggcggag aaggtcgtcc ggcctgcggt
     1741 ttggaatctt agccgtcact cggcggggat gtgtgcccga ttcgtgacgg atgctacact
     1801 catccgcgtg cgttactcgt tactttctgc taacctggcc atgccgcaca tgccggcaac
     1861 cggggtgagt ggcctcgatc tgtatgctca ggatgatcgc ggtgtgtggc gttgggttgc
     1921 cgtggcgcag cccacaaaac aggagatgga cctgacattg gtcagcggcg tacgtcctgg
     1981 aaaacgtcca ttcatgttgt atttgcccct ctacaatggc gttgagtcct tgcagattgg
     2041 tgtcccggaa aacgccgatt ttcagccagt accgccgcgg actgatggct tgatcgtcta
     2101 ttacgggacc tccattgctc atggcgcatg tgccagtagg ccgggcatgg catttcccgc
     2161 cattttggga aggcgagtca atcgtcccgt gatcaatctg ggcttttctg gcaatggccg
     2221 tatggaccta gaactcgttc catttctggg cgaacttgac gcggcagtgt tctgcattga
     2281 ctgcctaccc aatatgcagg ccgacgaaat caaagaacgt gcggaaccgt ttgtccgaag
     2341 cttgcgagag cgcaaaccac aggtccctat cgtcctcatc gaagatcgga cttacaccaa
     2401 cgcatggatc ttgcctgata gagaaaaacg tcaccaaaca agtcgcgagg ccttgaaagc
     2461 ggcgtaccaa aggctcttgg ccgaaggcgc gaaagagctt tattatgtgg aaggcgagca
     2521 tcaactgggt gacgatcatg aggcctgcac cgacggctcc cacccttctg acttgggtat
     2581 gatgcggatg gccgacgtct tggagcctgt tttacggcca ctgttgaagt aacaggcctc
     2641 attgacttag ccaaggcccg tctgaccact aaaaccaggg ctatttctgt gcgcaggatc
     2701 gatacgtttt tcatgttttt tcttgacaat agccgtgggt tctgggtcta attgtcgtat
     2761 gaagagggga gcggctacca ggcgcttctg actgcgagat ggggacacga agggcctcgg
     2821 cggagatcgg tctgatgctc ggcttcgtcc acgtgatgat tgaaccaacg ggggataagt
     2881 tttttgccgg gttgctcgtt gttgatcact ttggtgaccc gcacgaatta cgcgtcacta
     2941 aagccgttca gaccacggtc gttcagaaac tgctttgggg acaaaggctt ctcggccatg
     3001 ttttgcacaa ggtgatccta gtgcccttgg ccgggcaatt gggatctcag gtcgaagtga
     3061 tctttgtgga tagggcagag ttccttagcg ggagacagga tttatctgtc ccccttattt
     3121 tgctggaaag tcgagcggac attgaggaga gcgctcagtc tgaaacatcg gtaagtgtgc
     3181 ctgactcgaa gggcaatggg gtcttgctcc taagttgtgc gccgggcttt gaaacagatt
     3241 tggaggaagg tcggcggctg gtaactgaat tctcaaagac atgttacccc ctggaacctt
     3301 ttcaacgcgt cgaacacatt ttgaagcatc tgaagccaga ccttgaaact cgcacagggg
     3361 caaagggatg agaccgcgtt tccaacgggg ttggatgatt cgacttgtca cttcgattct
     3421 gtgttttctc gtcccgagca gccggcgccg cattcggaaa cctctcactg gtatccggtt
     3481 tcgcgtcgtg actcctccag cgaaccacga cgcggaacac ggcatggcac gaagctcgtc
     3541 ccgcctcgaa gggaactggt ggacaattga tgttgcggcg tttccacccg cagtggtgat
     3601 cagcaccaag tacgagagcc tcaagtctga ggtgcggaac gtcgctatcg ggcggcaccg
     3661 gccttcacct gcaatggttt tccctgtcga atgccggtgt cctgagcccc aagctctgga
     3721 tgtcgctacc gagcccgacc ggcctgcaat tgaaaaggtt tccgatctcg acttcgcacc
     3781 gccacaactt gacttaattc ccgtttcacc cgtatcggcc gaaatagtag agattccgac
     3841 tgagtcgttt gacatccctc tcatcatcgg cagggtacgt cgttcgggga gctcttcaga
     3901 ggagaaggcg tctcaacccc taaggccaac ctcgggaaag cttggtgact tggctcctaa
     3961 gccgatcatc ccccccgcct tgtgggagca aatttggaaa attctatcgc ctccacctcg
     4021 aattgaggaa attgtttccg aaaagtatcc ggggcaactc agaccctatc aggttgacgg
     4081 cgtgagacag ctcatagaaa aagagcgctt tattctcgcc gacgaaatgg gaacggggaa
     4141 gaccgttcag gtctgcgttg ccctttcttt attggttcga ctgggtcgca tccggcggat
     4201 tcttattgtc tgcccaaaga cagttctcac agtctggaca acccatcttc gtgactggac
     4261 cccggaaatt gtcgttcatt catttgatga tttctactca cctcgcgtag cggttaacga
     4321 tacgagggcc catgcctggg ttacgagcta tcctcgcatg ggtatgcaag ccgacgccgt
     4381 catctactgg ggtaccaaat gggacgttgt cgtcctggat gaggtccacg agattcggaa
     4441 ttcaaacaca aagaaataca gaaatcttca gatcattacc agccgggctc gctatcgatg
     4501 gggtctcagc ggaacaccgt tacaaaaccg gttggaagag ttaaccaaca tcttccaaat
     4561 cgttgtgccg gaactcaagt tacgggctga gacattgcag ctaaatcaag tgagggaaaa
     4621 gattcgcccg tggatgcggc gcgttactcg aaaggccgcc ctgccggacc tacccccgaa
     4681 agaacgtgtc gagatatggc ttaaactgga tcccgaacag gttcgggagt atgagttctt
     4741 cgcaggtaac gcagcacggc agatgccaat cggtgctgcg caaatgttca ctcatatttg
     4801 gcaagtcttg atgcacctga agcggatctg taattttgcg agtaattctc actccagtcc
     4861 gaagacccaa aaattggtgg agcttgtaga gcaaatcact cgaaggggcg agaaggtcgt
     4921 cgttttcacg cagttccgtc attatggagt cgatcgaata gctccgcact taaggcgttt
     4981 tggcctggcc gtcattcatg gagggtgttc cgacgccgag cggttttcgg cagtccaaca
     5041 atttcagaat aacccccaat gccgggtatt gctgggaacg gtgaaaacaa cggggtttgg
     5101 gctgacgtta acggcggcca atcacgtcat ccattttgat cactggtgga atcccgcggt
     5161 ggcgtggcag gcagaagatc gcgtttacag gattggccaa acccgtcccg tcaccgttta
     5221 cgagttctgg atgaaaggca cggtggagga gcgtgtgcga cagatcctcg aggagaaggg
     5281 attgctccac caagaggtta tcgaacggct ttcagacgcc gagttcaaga gactcttcac
     5341 actcgacgag ttgctcgccc tcctgcacct tcggccaagc tcactggacg cccacggcgc
     5401 agcatctcgg tcccaacaaa cgggcaccac aactttataa cgccttaggc tcttctcttg
     5461 accaagagcc tatcccataa tctcccctcg cccgttcggc gggagagggg tcggcggtga
     5521 gggtagctcg aatcgctgct ggttttctca atcgattccg acgatcgctt gccctctccc
     5581 caaccctctc ccggagggag agggggtact ttttgggata ggctccaaaa acaacggttg
     5641 gtttgctgag atttcatcga tcaataacct tcgaatcgcg tcaccgcccc taactgacca
     5701 agtccctcta cggggtggac tatgtttaga tgcgatcacc aattttattt gactctcatg
     5761 aggaaagaaa acaaactatg ggggaagcag ttcagatcgt ataaacgggt cgctaaggga
     5821 gttctagtaa tgcacgtgtt caagtcactc ctaacgtcta tggttgtggt ctcggttatt
     5881 agcagcgcca acggtgaagg cctctcgcag cctggcaacc ctagtccttc gcccaatgcc
     5941 gtgcgggcgg gtcgttttca cgtggagccg cccactttgc ttaatctcgg ctttgaatgg
     6001 gcaattgaag gagatgccaa tcgaaacgcg accgtggatg ttcgcttccg acaagtaggg
     6061 gaaatcgaat ggcgacaagc gctgcccctt gttcgcatcg gtggtgagag ggtgttccgc
     6121 gagcgagaag gcctggacta cactgtaccc gatggctttg caggcagcat cttgaatttg
     6181 caaccaggca ccgaatacga gtgccagttc cttatgcgag accccgacgg tgtggaggga
     6241 caagccgttc acaccgttcg tgtcaagacc cgatccgaac cgcagccgtt tcccggtgga
     6301 cgagtgctcc atgtttatcc gccggattac caaggggaac gacaggaacc cagtttttcc
     6361 agcattatgc aagcctacta cggcgccgga ttaggtgact ggaatgtggt gtgggaacac
     6421 cgtgtccagc cgggcgacac aattcttgtt catgcggggc tatacaagcc cgatcggcga
     6481 aactacgtgg acccgctgtg cacccccttc gacggcacaa cctatcttac gctcaaagca
     6541 acgccagacc gacccattac cattaaggct gccggtgacg gggaggtcat cttcgatgga
     6601 ggcggtaacc atcgcttgtt cgacgtgatg gcctccgcgt atcacatctt cgaagggctc
     6661 acattccgga ataccgatgt agccattttt gcgggacaaa aaggcgtaac aggggccgta
     6721 ggtctgacgg tgaaaaactg ccgatttgag aatgtgggct ttggtgtttg gaccgaaagc
     6781 ccttattccc gcgaattcta catcgccgac aacctcttca tcggtcgcga agatcgcttt
     6841 cggctcattg gctggacggg accgttgtgg ccaagtgcag gacgctatgg ttcgcatgag
     6901 ttggtgagct attacgcaat taaggtgtat ggcccgggac acgtgattac acacaacgcc
     6961 atcgcttatt tccatgatgg catcggcatt tcaacatatg gcacgccgga aaacgatcca
     7021 gacctcaggg catcctctat cgacatttac aacaacgaca tccacatgac gaatgacgat
     7081 tttatcgaaa ccgatggggc tgtacacaac gtgcgcgtct ttagaaaccg tggcgtcaat
     7141 gcggctcaag gaggttacag cgcgcagccg tcgtttggcg gtccggtgta tttctaccga
     7201 aatatcttgt accacgtgcc gacaggggtt tcctttaagt tctcggcaaa ccctgccggt
     7261 ctatttgtgt ttcataacac gattattggt gagcaggtgg gcatggggcc ctcgtcaaat
     7321 gtccattggc gcaacaatct gtttgttggc acggatgcac caggtcgcgc cattatgtcg
     7381 tggaacaacg caacggaaag ttatagctcc gactacaacg gctaccgacc aaaccgcggc
     7441 gtttccgccc agtataggtg gcgtgtgccg acagcggctc aaccccaggt ttttgccacc
     7501 ctggctgagt tccgctcggc aacagcccag gagaagcacg ggattgaagt ggattatgat
     7561 gtcttcgaaa atctcaggcc acctgatcct tcaaaccgtc atgcggtgta ccacgcgatg
     7621 gatctcaact ttcgccttaa tccgcacggt gccgcagtcg atgccggcgt aatcattccc
     7681 accgtcaacg aggacttcac cggaaacggt cccgatctgg gggccttaga attgggacaa
     7741 agcgaacccc actacggacc ccgatggctt acctggcaac cgttctatcg gtaatgcagg
     7801 attgcccctg ggattccgct cgaatctgat aaatttgtgg aatatgcctt taaaaagacg
     7861 tatcggatac catagaagag cggatcgcgg cttaaacaat cgacttgccc atcaatgctt
     7921 gtaagcgcga tatccaacgg cgaacgcttc gccgcgaggc cgttgatgcc gtggacgtgt
     7981 gacagccgat ggtggggtac aatcagggct cagcccatgt cttgatagca tcgttgggat
     8041 gacggaccta ttggacgcct ctgcaaggct cagtgaccta aaacgtgcgg cgacaggttt
     8101 aagttggtgt tcccaaaaaa gcccagccta ctccacccac cagccgctga aggccttgtc
     8161 ggttgtggag tgaattggaa aaacttaccg tgccggtttg tcactgcttt tacatttaca
     8221 acgtcgcacc acgaccgata ctagagcgaa tatgtcggaa tgcgcatggt ttccccgtct
     8281 ttgagggctg aaagatgcgc atagtatccg gggacggtca tatgcagggc gtcgataata
     8341 tccacggcag gtttccgatt acgcaagatg gcgtcaatga aatcatgggc taagtaaccg
     8401 tgcgaacctc cgtggccgcc tggccgaacg ttgggcggta atcccggttt cttcaatgat
     8461 aaaccctgag cgagggcctc ttcgtagcgt tttttccctg tctcgtcacc gatgaacttc
     8521 tggttgaacc cgccatattc accgcgaatc cgaccnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnatgtg cccaaagaca ttggccgcgt agagcttctc catcgcatac
     8701 aggtcatcgt gaaagacgga ggtctcgaac atcgcgtaga tgagcccacg gtttttcttg
     8761 cagcattcca agagacgctc ggcatcttcg atatcaccgt aaaaggcggg cacggctgta
     8821 gccacatgct tgccgtgttc caggcaaaga atggcatgcc gggcatggga gggtgcgtcg
     8881 gttgcgcaaa agatcgcttc aatggaatcg tccttgacca tttcttccaa agagggatag
     8941 gtcttttcgc actttgccag ctttgccatt tcggcacacc gttcggggat gagatcactt
     9001 acggctacca attcgacgtt gggatgatac tgcaaatcaa aggcgaggcc gaatttacaa
     9061 taaccgtgcc ccacaattcc caggcgaact ttgcgatctg tgtaaggttc ccatggtttg
     9121 ttggggtctg tttcgacttg gacctgctca aagccaggga tgggctcgcc tgaaacccct
     9181 cccgcaaaca cgccctgtga agccgccagg gctgaaccca tcaaccaggt tcgccgagaa
     9241 attttagaca tgatatattc tccttattcc tgggggcccg tagattcttt atcttctgcc
     9301 acgccttatt atccgcgtgc caaactgtgc ttgttcccaa aacccatcgc cgtcaccgct
     9361 gcccggccgg tgcagcattt ccccggaggc ccacgtcacc ggctcacatt ggtgatcggc
     9421 aattccgagt cgtttccgag tcgtgcggct agacttcgat tgtataaggt ggccgttgcg
     9481 gcctttttac atatatggat tgggcggctt cagcgtctcc tatgatctct tccttggtgg
     9541 cgtcccagta gatccgtcgc ttgaggcgga tggcaatatt cgccacgtga catgccgtca
     9601 aaacccgatg ggccacggcc actgtggagg ccggttgctt gcggcttttc acacaatcaa
     9661 aaaagttcgc catgtgatcg tcgctttcgt aaagacgaat ggcgttggcg ggaagtggat
     9721 tttctttcag ttcttcgacc gccttgccgt aggcgccacc acggttgaca aaaatacggc
     9781 ctttctcgcc gataaacata atgccgttgc gcgaagtctc cggcaggtcc agttgggcct
     9841 gattgaagag ctggacgtct tcctcgctgg tgatggtcgc ccgataacgc tcgtcgtgcg
     9901 ccacgaagta aagcaactcg atatctccgg gaaagagcat cttcacaaag aagcgatcgg
     9961 gattgtcgta acagtcgggt ttgccctcgt tggggaaaat ggcttcgcct tccacccaga
    10021 ggggaccgga gttttccatg tccatgcccc aataggcaat atccatatga tgctgccccc
    10081 agtcggtaat aatgccgccg gcatactcgg accaccagcg gaaattgaaa tgaactcgct
    10141 cgggatagta aggccgttcg ggggcttgtc cctgccataa atcccagttg aggtgaggtg
    10201 ggacggactg cggcttgaac ggtccaccct tggtgttcca aaaggggagt gtgacaatca
    10261 ctcgctgcaa cttgccgatg cggccatttc gaaccaactc acaggccagc cgaaagtgcg
    10321 ccacgctccg ctgttgggta cccacctgga cgatccgccg cgtttccgcc acaacctggc
    10381 ggaaaatttt cccttcttcg atggtcagac cgaggggttt ttcagtatag acatccttgc
    10441 caaggcggca cgccatcaca ttgatggccg tgtgccagtg gtctggcgtc ccattaagga
    10501 cgacatcgat gtcttgacgt tcctcgagga gcttgcggta gtcctgatag acggccggtt
    10561 tgccgtcaaa gagctggctt gccgcctcgg cgtgctgcaa gtcggcatcg cacacagcca
    10621 cgacatcccc gaaacgtgcc gccgacttga ggatggccgt cccacgtccc ccaacgccaa
    10681 tcgcggcgat gcgggggcgg tcgtttttct cctctccacc ctgacccatg acacgtgaat
    10741 gggtgaacca gtaaggcacc gccaaggcgg aggcggcggc taacatccga cgacggctaa
    10801 aacgaagacg ccgcatatca tggttcctcc ttcatcaacg gaacgcggtt tgcgcgcccc
    10861 tatcgctgct tatgtcgcca aggccatgga ccaaaagact tcttttgccc accaggcgtg
    10921 ctttagcata gtgcgcttta ttaccggttg taaagcgagg caaagcacgt ggcacgattc
    10981 gctcctgggt gtaggagcct aatctcccct cacccggtcg gcgggagacg ggtcgggcgt
    11041 gaaagtagct tgaatcgctt ccggttttct caatcaattc cgacgttcgc ttgccctcac
    11101 cccaaccctc tcccggaggg agagggggac ttttggggat aggctctacg tagcatcaag
    11161 catcaactgc tgggcgatat cgcaagcaag gtctattttt tctccgccgc tctttccacc
    11221 agtagcacgg gtcgcagaat gccacccaaa aaaagttcag taatttgcga gtgatccacg
    11281 cgcaccgcca ccacgttctc gcccgatgtg attgcagaag tgatatcgac ctcaaagggg
    11341 actcgctttt tcgggcttgt tccaacctct tggccattaa cgaagaccgt agcgtcacca
    11401 tcgacctcag tgaaaaagag gctcaaacgc ttggctgtct tcggcacgga aaagcgggtg
    11461 cgataccaca aaatagtctg cctgtcgggc aaaccctgag catccagcgg cttgctgtag
    11521 gtgaggacct catgccatcc cgaatcgtcg tagtctttct tgttgtagcc cagcgcgacg
    11581 ccttgatcgt cgggatcata ggtcatccgc cacttgtccg gcaacacgga gatcagtcgg
    11641 ttgggagaag ccgtgaccgc tgccccggcc ctgacctgcg gcccgagaaa ccgctccaag
    11701 tacgcaaccg tgtaatggtt gccccaacct tcttcaacgt tcttcttact tcgggcgagg
    11761 aggttttcgt acgtctgcaa ggccgtcaca aaatcacctc ggttcatggc ctggcgaatt
    11821 gtctcgtatt gtgccgcatt ttccagacct tcagccgcca tgcgaactcg tgccaagcaa
    11881 gcgggctccc cttctgctgc ggcagccgcc cgttccagca agtcccgaca gtgcttgagg
    11941 aagtctgggg tatagaccag gtggaccgca taaaaccccc cggcgtggca attcagatcc
    12001 acaaacgcac gatcaatgtc caaccaatac tgtttcatca aggggcccgc ccgaggtcca
    12061 tagaacttct cgaagtaatc gtccatgatg gcgtcggcat cggcttgagg atcataggcc
    12121 agccgaatgc tgagatagat gtgcggaccg taaatctgcc agttggccag tgtctcaaga
    12181 ttgatgccca agcaattttt gcttttcaga tacgggatgt catgtttcca gatggaaatc
    12241 atcgaaaacg gcacaagaca ttcggccaag ttgtagttgt aggtacggta acccaggttt
    12301 ttagcgatgg ccgcccacgc ttcgacaagc tgggcgagct gcatgcggct gggacaattg
    12361 ggatgaccga tgcggtgcaa ccggcaatag cggatggggg ccaaccatgc acacaggttc
    12421 ggttccaact ttgtcacgcg ggaaggcgcc tgcgtgtaat ctgcataaca gtagaagttg
    12481 agaatcgaat caggtgcggc ctgacggact tgtcgagcca catcctgaaa gaaatccaca
    12541 taacgagtgc tcaccgagac tcgaccactc gacggctcaa tgagatgagg atcatcttgg
    12601 gcaaggcatg ccgcgcactc gcaatatcct cggccgtccg gcggggagat cgaggggtgc
    12661 cgggtacctt tggcaatctc ggcaatcacc ccttgagcga agagatgccg cacttctggg
    12721 ttcgaggtgc aataccaatc cccggcacgc cgctggccag cccgtaaggc gaaatactcc
    12781 ggatgggtct caaacaagtc tttggaaatg taacggcccc aggcgtgctg cgtttgcagt
    12841 ggtaaaccac cggcaccgtt ccacactttc cacagggttt ggccactcca ctgcgagccc
    12901 caaattgacc ggtaaacaaa gctgggcttc tcggtgactt caagtctttc gatggtcaag
    12961 gtatttacct tgggatagac ctccccgaga ggatggtcca caaagtagcg acagccccag
    13021 gactcaagga gccgacacac cgcgcgggtg gtagcaatac cgctttgccc ggctaggagt
    13081 aaccggtcgc cgcggaggga aatgcgtagg ccctcttgcg atgggctatc aatgtcatca
    13141 agtttaagcc cggctttgat ggccaccgag ccgatgacaa tagccggttg gtctggcggg
    13201 accgagccaa ccaccgtcaa ccgcgcccct gtcatcttct cgacccattc aaccagtacc
    13261 tgggccgctc ggcgatcatc ccatgtgtca cctcgaaggc gtccgacgtt gctgggcctg
    13321 tcggccgttt cgaccactac gacagccacg gggcgaccat tgcggataac atctagcgcc
    13381 accccacgct gtgcattcac agtaactgcg acaacgcccg cgacaagaaa cgcaattgct
    13441 tcaaaccgcc gcataacagc ctccccgtgt ctttgccaat cgataagttg aaaacttcct
    13501 taagctgact caccataacg gctaatttat tttcaccctc gcaaaactaa aatatccagg
    13561 gccaccgact ttgtcacggc ggatggattc attttcgtga gaagatcccc aggttaagat
    13621 atgtgaacga ttgccttcag agctttccgg tcccaaaata tgatgtcaca atagatgagt
    13681 cgttcccgcc tgaaaaagat ttaacccgct tggacacgtc gcgagacaca cccttacctc
    13741 cggtcctgcc tcttgaaaag cgaaaggatt gagcatgaga ttccgacaaa tcccgctgtc
    13801 gttgatggtc ctgattgtgt tggcctacat agccgccttg caggggcaag gagtggctgg
    13861 ccaatccgtt gccgaggagc ctcccagtgc ccccaagtat gatacctacg tcaaggcacc
    13921 gcccccgcaa gagttccttc aacgattgcg aaagactcat ccgcgattga tccttcttga
    13981 tgaggagctg ccgaagatca aggcctttct cgagcaggac gtccggggga aagcgtggta
    14041 ccgcaacgct cgggagcgag ccgaggaact ttacgcccgg cctgtcgtta agcacgaact
    14101 ccccgatggc cggagattgc tttccatttc gcgggatgtg ctggaccgga tgtatcactg
    14161 gtgcttcttc tatcgcatgg aaaaagacaa gaagtggcta gaccgagcat ggcgggagat
    14221 ggaggtcgta gccgaatttc ccgattggaa tccctctcat tacctggaca ccgccgaaat
    14281 gatgcacgcc ctggccatcg gttatgactg gctgtaccaa gacttgaccg aggcccaacg
    14341 aaaggtgatc cgcgaggcct tatggcggca tggtctgcgg ctctcatact cggcgtacaa
    14401 ggggttgaag gcagaaggcc agcagagttg gacaaccgtg accaacaact ggaattttgt
    14461 ctgcaacggt ggttcgtcga tcgccgccat ggccctcttg gatgagatgc ccgacgagtg
    14521 tagcgaggtc ctttatcacg ccttccagcg catccagata ccactacagg ggtttgaacc
    14581 cgatggtgcg tggtgggagg gactcggcta ctggggatat tccatgcgtt acttttgccc
    14641 ttacttgcgg gcactagaga cggcattcgg taccgatttc gggtttattg aggcccttaa
    14701 gaacacggga tttgccaagg cgggcgactt tccggtgtat cttgtctcac cgttcgggac
    14761 caacttcaac tttgccgatt cgggaagtga cacttcccat ttttcccatt gggcattgtt
    14821 ctacctcgct gacaaatttg aaaatccact ttatcagtcc ttccaagagg agcgcaccag
    14881 cggcggactt caggatatcc tttatttccg ggatttaggc aaatcgggca acttggccgc
    14941 aatgccgttg gacaagcatt tccggggggc cgaattggcc tcgatgcgaa gttcctggac
    15001 cgatcccaac gccctttttg tggccatcaa gtgtggtaag aacgggattt cccatgccca
    15061 tcaggacttg ggaagtttca tcttctatgc cttgggtgag cgctggttca ttgatcctgg
    15121 cacagagtcg caaacctacc tgagtcacca gcaccatctg ccgcggagtc atttctaccg
    15181 cattcgagag gaaggtcaca acacgctggt gtttaatccc gatgaaggtt tttcccaaag
    15241 cggctcagga acatcaaaga ttattcgcat ggtttcttcg gctgatgagg cctttgccat
    15301 tgcagacctc accgatgcct accgccgtca tggagtatcc gtgaaaaggg gctaccgcct
    15361 gactcaaggg cggagcgtct ttctggttca agatgaaatc gagagtgacc acgacaacga
    15421 agtatggtgg tttgcccaca gtgcagcagg gacgaaattc agcctaaccg acgacggacg
    15481 gcacgccact ttggagcgaa gcggcaagac ctgccatgtc tatctgctgg cgccttcaga
    15541 tgccgtcttt caggtgatgg atgcgaaacc cctacccagc tctcctaacc ccagcatcca
    15601 gaatcccaat caggacatga agaaactcgc catccacttg cccaaggtcc agaatgttac
    15661 gttagcggtg ctcttcgtgc ccgtgccgtc gggtcaacgc ccgtcagtca caaccttgcc
    15721 cattcggccc ctggaaaact ggtaagatct accgagaccg gttggcaacg tgttgaggag
    15781 gattcggtca ttctgtgcaa gagagtgtca taaacacgcc ctgccctgcc ctgccgggaa
    15841 caatcaccac cggcatttcg gattttatgg ctctttctcc acatgggccg acttcgagat
    15901 ca
//
LOCUS       DAOSUT010000090        15738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2295_length_15738_cov_10.316011, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000090 DAOSUT010000000
VERSION     DAOSUT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_15738_cov_10.316011"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..73)
                     /locus_tag="PLD05_09520"
     tRNA            complement(1..73)
                     /locus_tag="PLD05_09520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Cys,seq:gca)
     gene            complement(278..1915)
                     /locus_tag="PLD05_09525"
     CDS             complement(278..1915)
                     /locus_tag="PLD05_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77721.1"
                     /translation="MTYASRTAFRPGTFWACLLALAVNLAVTPGGIGGEIAGSAGDTG
                     TTSAAAPQQLPPPVPRVENLKSTQQTKASPLSQGLSSRGDTTRPQSNASSPPVPIRPM
                     PSLPLTPGAGVSNPPGGQPTAQPIVAPETDRVEVKLEVSGKVGNPKTGTSNLKLEADL
                     VYEEQIIQTGDAVSQPVQALRLYENAEAKIWLNDSLIHPVLREDRRYLGVYFAEGKGE
                     LFCPEGPLTRDELDLVDVLGNTAVIDLLLPRTAVREGSEWDVPDQVVSVFLGLETVTS
                     NTVKCRVSEILPGGVIRVEMSGSLRGIVLGAVTQAELLARFQFPLSRRKIGWLGMVIR
                     ETRGASFVDHPFELTSRLQIVRNPANDPKVVKYRPGVPLTFPPLPEHLRLVHRDEAKG
                     WQLTYDRKWYLVSSSPDMTVFRMVDRQGQVAQCNIAPLASRETKLTLAGLRDTVKEIL
                     GENFGRVIDGNELTSPTGHQMFHVHVAGQVSDVPIHWIYYVMTTPDGKNHAVVFTVEE
                     KTLERFGNGDRELVDGFEFLQPAEVPRISQGPPTPIR"
     gene            2618..4324
                     /locus_tag="PLD05_09530"
     CDS             2618..4324
                     /locus_tag="PLD05_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP77722.1"
                     /translation="MTETLKVSTDYRIPAIRELCYQVVRFTPSAKKIEQTNRAEALLS
                     EIKPDKWYSYGWICYKITRFRPDNAGEQFLGEDLRHDLILFIEDVAETVPLRPEDVSE
                     KIYTLQELAKEFNVSTKTITRWRRAGLVSRRFLIDGRVRVGFLESTVKRFASQEHTRI
                     NRASQFSQVSPEERQAIIERARRLAQAGACRSEVVKRLAARTGRSMETIRYVLQQYDQ
                     ANPDMAIFPDARGPMSEETKERVYRDYRAGESVDVIAKRYCLTRARVMRVIDEMRAKR
                     IMDLPLDYIPNETFEKVTPEEEKQILGPPPPSPHPTRAAKLPPGLPPYLASLYEVPLL
                     TQEQEVHLFRKMNYLKFKASKLREQLRAEIDARKRPNRALMDEIERLYEESVKTKNEI
                     ISANLRLVVSIAKRHVGPAENFFELVSDGNMSLIRAVEKFDYSRGNKFSTYASWAIMK
                     NFARTIPDEHRYRDRFRTSQNELFTLTQDDRSDQIEQETSQLQREIQLQNILHRLDER
                     ERQIIIRRFGLDREQEPLTLKEVGAELGVTKERVRQLEARAIDKLRKLAEEEKIELSD
                     LE"
     gene            4734..4928
                     /gene="rpsR"
                     /locus_tag="PLD05_09535"
     CDS             4734..4928
                     /gene="rpsR"
                     /locus_tag="PLD05_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOP77723.1"
                     /translation="MVDGQYPRPLYVDYKDVELLKKLTNRQGKIVGRRKTGCSAVSQH
                     AITKAIKRARIMALLPFVGE"
     gene            5277..6161
                     /locus_tag="PLD05_09540"
     CDS             5277..6161
                     /locus_tag="PLD05_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOP77724.1"
                     /translation="MLDEIPFNGRRAYSYLQTICEIGPRPSGSPGMKAQQEMLTAHFR
                     KLGAEVTLQHFTGIDPRNRAPVPMANFIARFFPDRTERILLCAHYDTLPYPLRDRFNP
                     KGRFVGANDGGSGVAVLMELAHYMDKVPEKLGVDIVFFDAEEFIFSEWDPYFQGSEFF
                     ARSYVESRQQRKWRYRAAVLIDMVGDADLQIYQERNSAWWKDSRWILEGIWATAKRLG
                     VTEFVPQKKYEIRDDHLALHNIAGIPAIVIIDFDYPYWHTQEDTPDKCSPLSLAKVGW
                     VLLEWLSSLDVAKLPPVP"
     gene            complement(6596..9244)
                     /gene="clpB"
                     /locus_tag="PLD05_09545"
     CDS             complement(6596..9244)
                     /gene="clpB"
                     /locus_tag="PLD05_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOP77725.1"
                     /translation="MAAFRFDKLTIKAQEAVANAQSLATERGHPEIDPVHLLAALLSE
                     SDGIVKPLLDRIGVNWSQLNRMVEAELGHFPKVSGAAQTAPNTAFIKVLEQAQKEADT
                     LRDDYISTEHLLLGLSKIDSKARNILKLNAIGEQEILNAMRAIRGPARVEDQNPEAKF
                     QALERYGVDLVERARRGKLDPVIGRDQEIRRVMQVLSRRTKNNPVLIGDPGVGKTAIV
                     EGLAQRIADGDVPETLKNKRVIALDMGALVAGTKYRGEFEERLKAVLREVEAAGGNIV
                     LFIDELHTVVGAGRAEGGADAANLLKPALARGELRCIGATTLDEYRKYIEKDAALERR
                     FQPVYVGEPSVEDTIAILRGLKPRYEAHHGVKVKDSALVAAAQLSHRYITDRYLPDKA
                     IDLVDEAMSRLAMELKSVPVEIDQLQRRIMQLELAERQLADETEEHAKERRAEIAAEI
                     SRLREQLRELRAQWEKEKAALENIHRLRNELQQAEHEFNQLQASIKEKQSMGVIVREE
                     DYQRLYELDLRRRELAQQLEKAENNYPVTDDKRLLRLEVGPEEIAEVVSAWTGIPVSR
                     MMETERAKLLVLEERLHQRVVGQDEAVEAVANAVRRSRSGLQDPKRPIGSFIFLGPTG
                     VGKTELCKALAEVLFDDENAMVRLDMSEFMEKHTVSRLIGAPPGYVGYEEGGVLTEAI
                     RRRPYAVVLLDEVEKAHRDVFNILLQVLDDGRLTDNQGHTVDFTNTIIVMTSNIGSHV
                     IQEITESGGSEKEVQEAVLQLLKQHFLPEFLNRVDEVIIFRPLRRPQIKKIAEIQIRR
                     LKQRLEERGIHLEVTNRAIEALAELGYDPAYGARPLRRVIQRRIENPLAVELLKESNW
                     EGATIKVDYVGDEFVFEKVNGQASSA"
     gene            complement(9399..11303)
                     /gene="dnaK"
                     /locus_tag="PLD05_09550"
     CDS             complement(9399..11303)
                     /gene="dnaK"
                     /locus_tag="PLD05_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOP77726.1"
                     /translation="MAQEKIIGIDLGTTNSVVAVMEGKEVKVIPNAEGNRLTPSVVAF
                     TDRGEILVGEPAKRQAITNPKRTVYSIKRFMGRRREEVKAEEAIVPYEIVGDVGEYVK
                     VKIGDRTYTPPEISAMVLRKLKEAAEAYLGHRVNKAVITVPAYFNDAQRQATKDAGQI
                     AGLEVVRIINEPTAASLAYGLDNKEAEKIVVFDLGGGTFDVSILEVAEGVFRVISTNG
                     DTHLGGDDFDRALVDYVAEEFKKEHGIDLRKDPMALQRLQEACEKAKKELSTLQQTDI
                     NLPFIAFDASGAKHLHMTITRSKFEQLVHHLIERCRGPVEQALRDARLTPKDIDEVVL
                     VGGSTRIPKVQELVREMFGKEPHKGVNPDEVVAVGVAIQGAVLAGEVKDVLLLDVTPL
                     SLGIETLGGIMTKIVERNTTIPCTRKQIFSTAEDNQTAVTIKVYQGEREMAADNRLLG
                     MFNLEGIPPAPRGVPQIEVTFDIDANGILHVTAKDLGTGKEQKVRIEQSAGLSKEEIE
                     RMRREAELHAEEDRRKRELAELRNKADNLAWQIEKLMKEHEDKLSSADREALTRAIEK
                     ARNAAKSENAGEIKSAISELEAAAHAFSKTLYERAAAAAGATSTTGSGSSSSAGKKDD
                     DTIDAEFEVK"
     gene            11876..12661
                     /locus_tag="PLD05_09555"
     CDS             11876..12661
                     /locus_tag="PLD05_09555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOP77727.1"
                     /translation="MSQSQSWINFPGGTPEILYECKECLVVNKPRGILVQAPPGIPSL
                     AEMLRDFLATRQQPRTGIYLGVPHRLDRPVTGAILFTRHSRATRRITEQFESRMIRKV
                     YWAVVEGQVEPNVGIWRDFVRKIPNEPQAEIVPESHPDARHAVLNYRVLQRWENMSWL
                     EIHLETGRMHQIRIQASSRRHAVIGDWQYGSTADLMPEETDPRSKPIALHARELTFLH
                     PMTFQTISVTAPLPSYWSRLSPLFLQYTSLSTSASGSETNLID"
     gene            12839..15550
                     /gene="acnA"
                     /locus_tag="PLD05_09560"
     CDS             12839..15550
                     /gene="acnA"
                     /locus_tag="PLD05_09560"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOP77728.1"
                     /translation="MIADKNPFNAAYDAETLIGKCRLYRLTTLEDLGLVRLSDLPYSI
                     RILLECVLRNCDGYQVTEEDVRNLANWASHSGKTEVEIPFKPARVLLQDFTGVPAIVD
                     LAAMRSAVHRLGGDPKKVNPLIPVDLVIDHSIQVDFYGTPDAAAKNLELEFQRNKERY
                     QFLRWGQASFANLRVVPPGKGIIHQINLEYLAQCIFVAEKDGVRVVYPDTLVGTDSHT
                     TMINGLGVVGWGVGGIEAEAAMLGQPLSMLAPEVIGLELSGELQPGVTVTDLVLTVTE
                     LLRREGVVGKFVEFFGDGVSRMSVADRAVLANMAPEYGATMGYFPIDSQTLEYLRLTG
                     RAPEHVNLVEWYAKEQCLFRSPGDATPAYTKVLRLDLSTVVPSLAGPKRPQDRVPLHE
                     LKKSFQSSLRTPVEKRGFGLDESASQRSVKVNLDGQEVSLYHGSVVLAAITSCTNTSN
                     PALMIAAGLLAKKACERGLRVPAYVKTSLAPGSRVVTEYLQKAGLIPYLEKLGFAVVG
                     YGCTTCIGNSGPLPDSIVEAAQQQIVLAAVLSGNRNFEGRIHSAVKANYLLSPPLVVA
                     FALAGSVNKDLTSEPLATDTQGKPVFLKDIWPTEDEIRDAVQRSVSPEVFRQKYADLF
                     EGESLWEDLTIQASELFPWSEESTYIHEPPYFKDFTLREPGVPSIQGARVLLALGDSV
                     TTDHISPAGSIPKTSPAGRYLIERGIPPHEFNSYGARRGNDLVMVRGTFANVRLRNFL
                     AAGTEGGFTRFWPTGEVMPVFDAAMRYKELGTPLIVLAGSEYGTGSSRDWAAKGPALL
                     GIRAVLAASFERIHRSNLVGMGILPLQFPSGTTWQSLGLTGEEVFDIVLPQGGLTPRS
                     SVRVVARKPDGQVLEFACIARIDTPVELDYYRHGGILPFVLRRLLQS"
BASE COUNT         3429 a         4387 c         4282 g         3640 t
ORIGIN      
        1 aggcgccggt cggattcgaa ccgacgaatg gcggatttgc aatccgctgc cttagccgct
       61 tggctacggc gccaacattt cgacacttca cttttatgca acttcaacct tattatgtcc
      121 cgtcatccct agcaaggcaa gaggcatcgc aaaacgctcg agcacgcgcg ggaaatcgac
      181 attgaccaac tcaacgtgct cctttgctaa gtcatctact gtcacgtttg gcaggaaggc
      241 gaacttgggg gacaactccc accttctaga gataacttca tcgaattggc gtcggcgggc
      301 cttgggaaat tctcggaacc tcggccggtt gcaagaattc aaaaccatct actaattccc
      361 ggtcaccatt tccaaaccgc tccagcgtct tctcttccac cgtgaagacg acggcatgat
      421 ttttaccgtc aggcgtcgtc atcacgtagt agatccaatg gatgggaaca tccgaaacct
      481 ggccagctac gtgcacatga aacatctggt ggcctgtcgg acttgtgagc tcgttaccgt
      541 cgataactcg cccgaagttc tcaccaagaa tttccttcac cgtgtcacgg aggccagcaa
      601 gtgtaagttt cgtttcccga gaagccaacg gggcgatatt gcactgggca acttgccctt
      661 gtcgatcgac cattcgaaag actgtcatat ccggcgaact tgagacaaga taccacttgc
      721 gatcgtaagt gagctgccaa ccttttgcct catcccggtg taccaacctc aagtgctctg
      781 gcaggggtgg aaacgtgagg ggtaccccag gtctgtactt gaccaccttc ggatcgttag
      841 cggggtttcg cacgatttgg agtctggagg tcagctcgaa agggtgatcc acaaagctcg
      901 ccccacgtgt ttctcgtata accatcccta accagccgat cttccgccgc gataacggaa
      961 actgaaacct cgccaaaagc tctgcctgcg tcactgctcc aaggacgatc ccgcgaagac
     1021 ttcccgacat ttccacgcgg atgactcctc cagggaggat ttcgctgacg cggcacttga
     1081 cagtgtttga tgtgaccgtt tctaaaccga gaaagacgct caccacttgg tccgggacgt
     1141 cccattcact cccttctcga acggccgtgc gcggaagtaa caagtcgatg acggcggtgt
     1201 ttccaagcac gtccactagg tcaagttcgt ctcgtgtgag cggtccctcg ggacaaaata
     1261 actcgccctt cccttctgcg aaatagacgc ccaggtaacg acggtcttct cgcagcacgg
     1321 gatggatcaa agaatcgttt agccaaatct tggcctcagc attttcatag agacgcaggg
     1381 cctgaacagg ctgggaaacc gcatccccag tctggataat ctgctcctcg taaaccaggt
     1441 cagcctcaag cttcaggttc gaggtgccgg tcttcggatt gcccaccttg ccagaaactt
     1501 ccagttttac ctcgacgcga tcggtctcgg gtgcaactat tggctgagct gtgggttggc
     1561 cccccggtgg gttcgaaacc ccggcaccgg gagttagagg gagggaaggc attgggcgaa
     1621 tcggcaccgg cggactgctg gcgttcgact gcggccgggt ggtatcgcct cgcgatgaca
     1681 acccctgcga aaggggggac gctttcgtct gttgggtact cttgaggttt tcgacacggg
     1741 gtacgggcgg aggtagttgc tgaggcgctg cggctgaggt cgtgcctgtg tcaccggccg
     1801 agccagcgat ttcgccacct atccctccag gggtgacggc caagttaact gccaaagcca
     1861 atagacaggc ccaaaaagta ccaggtcgga aggcagttcg gctagcataa gtcatggaga
     1921 gcatcgagaa catagggagt ctacgatatt tggataactt aagcagcgaa tatcgttgcc
     1981 gaggttcact ccatccccgg aaaactaagg gcgggcactg taaccaggag gctgttcgcg
     2041 tgtcaatagg gcttggggag ctaccgccga acgggccaaa cgccattgca agcttcgtcc
     2101 tgtgccgcat tttgaagcat gctgtgccgg atttgcagga taaaacgaaa cgagctcctg
     2161 atcttagaca cagattgtac atatttacat tggcggcaga ttttagttga ttgccaaagt
     2221 gaatattcgg tgtcatttat cgctaaggaa cacaatttgc agagctgatg agttcgaagc
     2281 tggaggggga cgattaggtg ttggtttagt ctcggccgcc ggcgggatag gtttctagga
     2341 gattaccctg ttcggctttc tgtatattgc catagtgcgg ttggggggga cgctctggac
     2401 tggcccatcc tgggcacaaa agtgcgatcg tttttggcac aggaggtctt tgacgaaatg
     2461 cgtgggccgt gtaaactcta ttgtatcaaa tcaatctgac ataattgcat aaagacctcg
     2521 aaccatttca tggtaattga cgtcctatta cgggcaggtt ggggcgaata ccaggaaggg
     2581 gaaaacactt tgcaagcaag gcgatcggat gacaccgatg actgagacac tgaaagtcag
     2641 cactgattac cgaatcccgg ccatcagaga gctgtgctac caagttgttc ggttcacgcc
     2701 ctctgccaag aagattgagc aaacgaatcg ggcggaggca ctcctgagcg aaatcaagcc
     2761 ggacaagtgg tactcttacg ggtggatttg ctacaagatt acgcggtttc gaccagacaa
     2821 cgcaggggag caattcctgg gcgaagacct tcggcatgat ttgatcctgt tcattgagga
     2881 cgttgcagag accgttcctc ttcgcccgga agacgtttca gaaaagattt acacgcttca
     2941 ggaacttgcc aaagaattca acgtctcaac aaagactatc acaaggtggc gccgtgccgg
     3001 cttggtgagt cgtcggttcc tgattgacgg tcgtgtacgg gtgggttttc tggaaagcac
     3061 cgttaagcga ttcgcgtcgc aggagcacac aagaatcaat cgtgcatcac agtttagtca
     3121 ggtctcgccg gaggaaaggc aggccattat tgaacgggca aggcgactgg cccaagccgg
     3181 ggcctgtcgc tccgaagtcg tcaaacgtct agccgcaagg acggggagaa gtatggagac
     3241 aatccgttac gtgttgcaac aatatgacca ggccaatccg gacatggcca tctttccaga
     3301 tgcgcgcggc ccgatgagcg aagagaccaa ggagcgcgtc tatcgggact atcgcgcagg
     3361 ggagtccgtc gatgtcatcg ccaaacgcta ctgtttgacc cgggcgcggg tgatgcgcgt
     3421 catcgacgag atgcgggcca agcgcatcat ggatttaccg ctagattaca tccccaacga
     3481 gacattcgaa aaggtaacac cggaggaaga aaaacagatt ttgggaccgc ctccaccttc
     3541 gcctcatccg actagggcgg ccaaactacc tccaggatta ccgccctatc tcgcgagtct
     3601 ttatgaagtc cctctcttga cgcaagagca agaggtacac ctcttccgca aaatgaatta
     3661 cctcaagttc aaggcaagca agctccggga gcaactccgt gccgaaatcg acgcccggaa
     3721 gcgccccaat cgcgccctca tggacgagat cgagcggctt tacgaagaat ctgtcaagac
     3781 aaagaacgag attatctccg ctaatctgcg attggttgtg tccatcgcca agcggcacgt
     3841 tggtcccgcc gagaatttct ttgagttggt gagcgatggc aacatgtccc tgattcgggc
     3901 cgtagaaaag tttgattatt ctcgcggcaa taagtttagc acgtacgcaa gttgggcaat
     3961 catgaagaat tttgcccgaa caataccgga tgaacaccgt taccgggatc gcttccgaac
     4021 tagtcagaac gagctgttca ctttgacgca ggatgacagg tctgatcaga tagaacagga
     4081 gacatctcag ctccagcgag agatccagct ccagaacatc ttgcaccggc tcgatgaacg
     4141 ggagcggcag atcatcattc gccgctttgg tctggatcgg gaacaggaac cgctcaccct
     4201 caaggaggtc ggtgccgagc tgggggtgac caaggagcgc gtccggcagc ttgaggcccg
     4261 cgcgatcgat aagctgcgaa aactggccga agaggaaaag atcgaactct ccgatctgga
     4321 gtaaactggc caatacgttc gagttttcgc ggtgatgagg tggggccacc gctgtgccga
     4381 gggtcgcacc agattaaatc ggcctgactc caacaacgag cggttccaac ctattcggcg
     4441 gccatcaggc cacaaaatgg aggggccaaa gaaaaagagg gcaggcggga cgtattgtga
     4501 ttggcagaat ggctcttccg ccactgattc gccctcctac agaggtgggg cggtggggct
     4561 ctcttgtctc tggcgggaat tttggtaact tgatctttat gagggcacca agcgatatat
     4621 cgcggtgccg cggttgggta ttgtcgatga ttggctttgg gaaatcagag gatctctgaa
     4681 aacaaatggc gaaaaagcga attcggacga agcggcggaa acgtgaccct ttgatggttg
     4741 atggccagta tccgcgtcct ctgtatgttg attacaaaga cgtggaactt ctgaaaaagt
     4801 tgacaaaccg ccagggcaag attgtgggtc gtcgcaaaac gggttgctcc gccgtaagtc
     4861 agcacgcaat cacaaaggcg atcaaacgcg ctcgcatcat ggccctcctg ccgtttgtcg
     4921 gcgagtgaac ggaccgctta ttctcacggt ttgggcgcaa gcggacggca tggcgggcgt
     4981 tcatcagccg ggctgagacg ttcatagcca agatcgggcc cgtaatgtct aggcacagtc
     5041 gtagaaagat cggcccttcc gcggaagctc cgaaacaagg tcttcctatt agtcagtatc
     5101 ccagcgatcc tcggaggaaa cttgctgggt cagacgccgg tgattccaat acaacgacca
     5161 aaaagatcgc cgctgtgcgg cagcttggct tctttctggc aagtcttatc ctggtaggtt
     5221 gcgcaggatt cgttgcgtgg cagggatttc cattccgtgg agggcgctct cggctggtgt
     5281 tagatgaaat cccttttaac ggccgtaggg cttacagtta tctgcaaacg atttgcgaga
     5341 ttgggccgcg tccgagcggc tccccgggga tgaaggccca acaggaaatg ttgacggccc
     5401 actttcggaa gctgggggcg gaagttacgc tgcaacattt tacgggcatt gaccctcgta
     5461 accgagcccc cgtgcccatg gccaatttta ttgcccgttt ttttcccgat cgcaccgagc
     5521 ggatccttct gtgcgcccac tacgacacgt taccttatcc cctccgtgac agatttaacc
     5581 ccaagggtcg gttcgttggc gccaacgacg ggggcagcgg tgttgccgtc ttgatggaac
     5641 tagcccatta catggataaa gtgcccgaga aactgggcgt tgacatcgtc tttttcgatg
     5701 cagaggagtt tattttttcg gaatgggacc cgtattttca gggatctgag ttttttgccc
     5761 gatcgtacgt agagtcgcgt cagcaacgga aatggcgtta ccgggcagcg gtcctaatcg
     5821 atatggtcgg ggatgctgac ctacaaatct accaggagcg aaatagcgcg tggtggaaag
     5881 actctcggtg gattcttgag gggatttggg cgacagccaa acggctcggc gtgacagagt
     5941 tcgttcccca aaagaaatac gaaatccgcg acgatcacct ggcattgcac aatattgctg
     6001 gcatccccgc cattgtgatc attgattttg actatcccta ctggcatacg caggaagata
     6061 cccccgacaa gtgctcgcca ttgagtctgg cgaaagtcgg gtgggtcctg ctggagtggc
     6121 tttcctcttt ggacgttgca aaactcccgc ctgtccctta agggtattag aaggctacac
     6181 ggcgtcgtga ctgtatccca tgatgggtgc ccaccgggtc tgatatggga cgcgacagcg
     6241 cacgcttttc ccaattgagc accatggccc ctccaaattt agagcctatc ccataatctc
     6301 ccctcacccg ttcggcgtgg gaagggtcgg gcgttagggt ggctcgaatc gctgcccgtt
     6361 ttctcgatcg actccgacga tcgcttgccc tcacccaaac cctctcccgg agggagaggg
     6421 ggggactttt tttgggatag gctcctaatg ccgggttgcg cctcaattga ccgtacggcc
     6481 cttcacgccg caacattttt cgcggtccct gataccaaag ttatcacttg cgagggagag
     6541 aacgccgaaa atgtttcagg tggcgttccc gctcagggga ggttactgaa aaccatcaag
     6601 ccgagctggc ctggccgttg accttctcaa agacgaactc atcccctacg taatcgacct
     6661 tgatcgtggc accttcccaa ttggattctt taagcagttc cactgccaag gggttttcaa
     6721 ttcgccgttg gatcacgcgt cgcagcggcc tggctccgta ggcggggtcg tagcccaact
     6781 ctgccaacgc ttcgatagcc cgattcgtga cctcgagatg aatacctcgt tcttcgaggc
     6841 gttgtttcag gcgacgaatt tgaatttcgg cgatcttctt aatctgcggt cttcgcaacg
     6901 gccggaagat gatcacttca tcaacgcgat tgaggaactc gggcagaaag tgttgcttaa
     6961 gaagttggag gacggcctcc tgaacttcct tctccgatcc gccggactcg gtgatctcct
     7021 ggatcacgtg gctaccgatg ttgctcgtca tcacgatgat cgtgtttgtg aagtcaacgg
     7081 tgtggccctg gttgtcggtg agccgaccgt catccagcac ttggagcagg atgttgaaga
     7141 catctcgatg ggccttctcc acctcgtcca acaggacaac agcgtaagga cgccgccgaa
     7201 tcgcctctgt aaggacgccg ccctcctcat atcccacata gccgggaggg gcaccgatga
     7261 gtcggctgac ggtgtgtttt tccatgaact cgctcatgtc gagccgaacc atggcgttct
     7321 catcatcaaa gaggacctcc gccagggctt tacaaagctc cgtcttaccg acacccgtcg
     7381 gaccgagaaa gatgaacgac ccgatgggac gtttcgggtc ttgcaagcca gatcggctcc
     7441 gccgcacggc gttggccacg gcctccaccg cctcatcctg gcccacgact cgctgatgga
     7501 gtcgctcctc caggacgagc agctttgcac gttctgtttc catcattcgg gaaacaggaa
     7561 tcccggtcca ggcgctgacg acctcggcga tctcctcggg tcccacttcc aaacgaagca
     7621 atcgcttgtc atccgtcacc ggataattgt tctcggcctt ctccaattgc tgcgctagtt
     7681 cgcggcgacg taaatcaagt tcgtagagtc gttggtaatc ttcctcccgg acaatcaccc
     7741 ccatggattg cttttctttg atactcgcct gaagctgatt gaattcgtgt tccgcctgct
     7801 gaagttcatt gcggaggcga tggatgttct ccaaggcggc cttttctttt tcccattgag
     7861 cccgcagttc cctgagctgc tcacggagac gactgatttc cgcggcaatc tcggcgcgtc
     7921 gttctttggc gtgttcctcc gtctcatccg ccagttgtcg ttctgcgagc tctaattgca
     7981 taattcgccg ctggagttga tcgatctcca cggggacgct cttcaattcc atggccaacc
     8041 ggctcatcgc ctcgtcaacc aggtcgatgg ccttatccgg aagataacga tcggtaatat
     8101 agcgatggga aagttgtgct gccgccacta gggcggaatc cttcaccttc acgccgtgat
     8161 gggcttcata ccttggtttg aggccgcgaa gaatggcaat ggtatcctca acggaaggct
     8221 cgcctacata aaccggctgg aatcgccgtt ccaaggcggc atccttctcg atgtactttc
     8281 gatactcatc cagggttgtc gcaccgatgc aacgaagctc gccccgcgcg agagcaggct
     8341 tcaacagatt ggcggcgtca gccccacctt ctgcccgacc agctcctacc accgtatgga
     8401 gttcgtcaat gaagagaacg atgtttcccc ctgcagcttc gacttcccgc agaactgcct
     8461 tgaggcgttc ttcaaactcg ccacgatatt tggtaccggc aaccaatgcc cccatgtcaa
     8521 gggcaatgac tcgcttgttc ttcaacgttt cggggacgtc gccgtccgcg atgcgctgag
     8581 ccaacccttc cacgatggcc gtctttccaa cacccggatc gccgatcaac acgggattgt
     8641 ttttggtccg tcgggaaagg acctgcatta cccggcgaat ctcttggtcc cgtccaatga
     8701 cgggatcgag ttttccccgg cgggcccgct caacaaggtc cactccatac cgttccaagg
     8761 cctggaattt cgcctccgga ttctgatctt ccacgcgagc cggcccccga attgcccgca
     8821 tcgcattgag aatctcctgt tcgccaatgg cgttaagttt caagatgttt cgcgccttgg
     8881 aatcaatctt ggacaggccc agcaacaagt gttctgtgga gatatagtca tcacgaagcg
     8941 tatcagcctc tttttgggcc tgctctaaca cctttataaa ggctgtgttg ggggcggttt
     9001 gagcagctcc cgagaccttt ggaaaatgac ccaactccgc ctcgaccatc cggttaagtt
     9061 gtgaccaatt cacccctatc cgatcgagaa ggggtttaac aataccgtcc gactctgata
     9121 aaagcgctgc gagcagatgt accgggtcaa tttccgggtg gcccctttcc gttgccaaag
     9181 actgagcgtt tgcaaccgcc tcttgtgctt tgatcgtcaa tttatcgaat cgaaaagctg
     9241 ccattgttta ttactcccct tgatatttcc ctgaaaaaat tcttgactag aagccataag
     9301 tatcttgtaa aaagggccgc tttccgtggt catgacgcaa gaaagcggcc cttcgcgttg
     9361 ctcaaaaact cgagggacgc gaaccagcgc tgccgatgtt atttcacctc gaactctgca
     9421 tcgatggtgt cgtcatcctt cttgccagca ctgctggacg atcctgaacc ggttgtcgat
     9481 gtggccccgg cagcggccgc cgctcgctcg tagagcgtct tgctaaaagc atgggcggca
     9541 gcctccagtt cgctgatcgc tgacttgatt tccccggcgt tctcgctctt ggccgcattt
     9601 cgggcctttt cgatagcacg ggtcaatgcc tcgcgatcgg cactggagag cttgtcctca
     9661 tgctctttca tgagtttttc aatctgccag gcgagattat ccgccttgtt gcggagctcg
     9721 gccaattcgc gcttgcggcg atcctcctcg gcatggagtt cggcctcgcg ccgcatcctt
     9781 tcgatctctt ccttggacaa accagcggac tgctcgatgc ggactttttg ttccttgccc
     9841 gtaccgaggt ccttggccgt cacatgcaag ataccattag catcgatatc gaatgtgacc
     9901 tcgatctgag gcacaccgcg cggggcagga ggaatccctt ccaaattgaa catgcccagt
     9961 aaccggttgt cggcggccat ctctcgttca ccctgataga ccttgatcgt cactgccgtt
    10021 tgattgtcct cggccgtgct aaagatttgc ttgcgggtac aaggaatcgt tgtgttccgc
    10081 tcgacgatct tggtcatgat gccacccagg gtttcaatac ccagcgacag cggcgtgacg
    10141 tcaagcaaca agacatcctt gacctcgcca gctagaacag ccccctgaat ggccacccca
    10201 acggccacca cctcgtccgg gttgacgccc ttgtgcggct cctttccaaa catctcgcgg
    10261 actagttcct gaaccttggg aatccgcgtt gaaccgccca cgagcacgac ttcgtcgatg
    10321 tccttggggg tcaaccgagc atcccgcagg gcctgttcca ccggaccacg gcatcgctcg
    10381 atgagatggt gtacgagttg ttcgaacttc gagcgcgtga tcgtcatatg gaggtgcttc
    10441 gccccgctgg catcgaaggc gatgaaaggc aaattgatat ctgtttgctg caaggtgctc
    10501 agctccttct tagccttttc gcaggcctct tgcagccgtt gcagtgccat gggatccttc
    10561 cgcaggtcga tgccgtgttc cttcttaaac tcctccgcca cataatcgac gagggcgcgg
    10621 tcgaagtcgt ccccacccaa atgcgtatca ccattggtgc tgatgacgcg gaagacaccc
    10681 tcggcgactt caaggatgga tacgtcgaac gtgccacctc ccaagtcgaa cacgacgatt
    10741 ttctcggcct ctttgttgtc cagcccgtag gccagagatg ccgccgtcgg ttcgttaatg
    10801 attcggacca cttccaaccc ggcaatttga ccagcatctt tcgtggcctg ccgttgggca
    10861 tcgttgaaat aggccggcac cgtaatgacc gccttattga cccgatggcc caaataggcc
    10921 tcggccgcct ctttcagttt acggaggacc atggccgaga tctcgggcgg cgtgtatgtc
    10981 cgatcaccaa tcttcacttt gacgtattca cccacatccc cgacgatttc gtaggggaca
    11041 atggcctctt cagccttgac ctcttctcgc cggcgcccca tgaaccgttt aatcgaatag
    11101 acggtgcgtt tgggattggt gatggcctgc cgttttgccg gctcacccac cagaatctca
    11161 ccgcgatccg taaaggcgac gacactcggc gtgagtcggt ttccttcggc gttggggatg
    11221 actttgactt ccttcccctc catcaccgcc acaaccgagt ttgtcgttcc caggtcgata
    11281 ccgataattt tctcttgtgc catgtgaata gctccctgtc tgcattctct ccttgttgcg
    11341 gaccgttacc caccaaaacc acgttccgcg agatgttcgt attggtagaa atccccgaga
    11401 tgcatcaacc cattgacgcg atcacgagtt cggtaaaaac ttacgttaat accgcatgag
    11461 caaatgatat gccggaaagc gaaaaagcgg agggacgctt cataagttga ctatttgccg
    11521 atacttataa gcacgcacgt gttatggtcc gcagccggaa ctatgcccga ttgaggagta
    11581 tctgccaatt tggcagtatg aagatagggg cggctcaata ccacctaaat ttggcggagc
    11641 gccctgccta atttgcgggg tgccggaata ggcgatggcg aaaccaatgg agaaaccctg
    11701 atcgccggcc gattgagctc ccacccgact cgccgtggcg catgggtcgg tgggtgcccg
    11761 taggctgata atagcttcgc actagctcgc cacgaaatct cccaccggga gtcacttagt
    11821 ccgttaaatt aggatattta tcatcttaga catgattcct ggacagcaga gcacgatgtc
    11881 gcaatcccaa agctggatca attttcccgg gggaacgccg gagatccttt acgagtgcaa
    11941 agagtgcctc gtagtcaaca aaccccgggg gattctcgtc caagccccac cggggattcc
    12001 ctcgttggcg gaaatgctcc gcgactttct ggcaacgcgc caacagccgc ggaccggcat
    12061 ttatcttggt gttccccatc ggcttgatcg tcctgtgacg ggcgccatcc ttttcactcg
    12121 gcacagtcgg gccacgcgac gcattacaga acaatttgaa tctcgtatga ttcgaaaggt
    12181 ctattgggcc gtagtggagg gccaagtcga acccaacgtc ggcatttggc gggattttgt
    12241 gcgtaaaatc cccaatgaac cgcaggccga gatcgtcccg gaatcccatc ccgatgcccg
    12301 tcatgctgta ctcaattatc gagtcctcca acgctgggaa aacatgagct ggcttgaaat
    12361 ccatcttgag actggccgga tgcatcaaat ccgcatccaa gcctcttcac ggcgccatgc
    12421 tgtgattggt gattggcaat atggcagtac ggccgaccta atgccggagg aaaccgatcc
    12481 ccgatcaaaa cctatcgctt tgcatgcccg cgagttgaca tttcttcacc ccatgacctt
    12541 tcaaaccatt tccgtcacgg caccgttgcc ctcttactgg agtcgccttt cgccgctttt
    12601 tttgcagtac acatctctgt ccacttccgc cagtggttca gagacgaatc tcattgattg
    12661 aaggggttca acgcggtcac ccgttggctt cggtttctcc ccgccctacc cacgaggtcc
    12721 catggaaagt aggataccgg aacggtcatc aatggcagga ggttgctccg tttaatagct
    12781 ttcgtagccc atcctaggta tttgtcgttc atctcaatta ttccgaagag ctaggtccat
    12841 gatagcagac aaaaatccgt tcaacgccgc atacgacgcc gaaacactga ttgggaagtg
    12901 tcgtctctat cgtctgacaa cactcgagga tcttggcctt gttcgcttga gtgacttgcc
    12961 ctattcgata cgaatcttgt tggaatgcgt gttgaggaac tgtgacggct accaggtcac
    13021 tgaggaagat gttcgcaact tggcaaattg ggccagtcac agcggcaaaa cagaggtcga
    13081 aatccccttc aaaccggccc gggtgctgct tcaggacttc acaggcgtgc ccgccattgt
    13141 tgatctcgcg gccatgcgaa gtgccgtcca tcgccttggc ggagatccga aaaaggtcaa
    13201 tcctcttatc ccggtcgacc tagtcatcga tcactcgatt caagttgact tctacgggac
    13261 gccggacgcc gctgccaaga atctggaact tgaattccag cgaaacaagg aacggtatca
    13321 gttcttgaga tggggtcaag cgagctttgc caacctgcgg gtggttcccc cgggaaaggg
    13381 aatcatccat cagatcaatc tcgagtacct ggcccagtgt atcttcgtcg cggaaaagga
    13441 tggcgttcgt gtcgtctacc ctgacacgct tgtggggacg gacagtcata ccacgatgat
    13501 caacggtttg ggggtggttg gctggggagt gggcggcatt gaggccgagg cagccatgct
    13561 cggtcagcct ctttcgatgc tcgcgcctga agtgatcggg ctggagttga gcggagaact
    13621 tcagccgggc gtcacggtca cagacttggt cttgactgtg acggaactcc ttcgccggga
    13681 gggcgtggtt ggaaaattcg tcgaattctt tggcgatggc gtcagccgca tgagtgttgc
    13741 agatcgggct gtgttggcca acatggctcc cgaatatgga gcgacgatgg gctactttcc
    13801 gatcgattcc caaaccctag aatacctccg cctgacaggg cgcgcgccgg agcacgtcaa
    13861 cctggtggaa tggtacgcga aggaacagtg tctgttccgt agtcccgggg acgcaacccc
    13921 ggcctatacc aaggtcttga ggcttgactt atcgacggtg gtcccttcgc tggcaggtcc
    13981 aaaacgccca caggatcgag tgcccctcca cgagttgaag aagagttttc agtcatcgct
    14041 acgaacgccg gtggaaaaac ggggcttcgg cctggacgaa tctgcgtcgc agcggtcggt
    14101 caaggtgaac ctggacgggc aagaggttag cctttatcac ggttctgtgg tcctcgccgc
    14161 aatcactagt tgtaccaaca cgagtaatcc cgccttgatg atcgctgcgg gactattggc
    14221 caaaaaggct tgtgaacgcg gtctacgtgt accggcttat gtcaagacca gcctggcgcc
    14281 gggttctcgc gtggtgacag aatacctgca aaaagcgggg ctcatcccat atcttgaaaa
    14341 actgggtttt gccgtggtcg gatatggatg taccacttgt atcggtaata gtggtccctt
    14401 gccagattcc attgtcgagg ccgctcagca acagatcgtc ctcgcagcgg tgttgagtgg
    14461 caatcggaac ttcgaaggtc gcatccattc cgccgtcaaa gcgaattatt tactttcacc
    14521 gcctttggtt gtggcatttg ccttggccgg gagcgtcaat aaagacttga cttcggaacc
    14581 cttagcaacg gacacgcagg gaaaaccggt ctttttaaag gatatttggc ccacagaaga
    14641 cgaaatacgg gacgccgttc agcgatcggt atcgccggaa gtgtttcggc aaaaatatgc
    14701 ggatctcttc gaaggcgaaa gcctctggga agatttgacg atccaggcga gcgaattgtt
    14761 tccgtggtcc gaagaaagta cctacatcca tgagccaccc tattttaagg atttcactct
    14821 gcgggaaccc ggtgtcccat ccattcaagg ggcacgagtc ctgttggcct tgggtgactc
    14881 ggtgactacc gatcatattt ccccggcagg cagtatccca aagacgagcc cggccggacg
    14941 ctatctaatc gaacgaggga tacctcctca cgagtttaac agttatgggg cccgccgcgg
    15001 caacgacctg gtcatggtca gaggaacctt tgccaacgtg cgcctgcgaa attttttggc
    15061 cgccgggaca gaaggaggat ttacgcggtt ttggccaacc ggggaagtga tgccggtctt
    15121 tgacgccgcc atgcgatata aagagcttgg cactcctttg atcgtccttg ccggtagcga
    15181 atacggcacg ggaagcagtc gcgattgggc ggccaaaggc ccggccctcc tcggcattcg
    15241 cgccgtcctt gctgccagct ttgaacgcat tcaccgcagt aacctggtgg gaatgggcat
    15301 tctacccctc caatttccct cgggcacaac ctggcagtcc cttgggctaa ccggagaaga
    15361 ggtgtttgac atcgtactgc cccaaggtgg actcactcct cgatcttctg tccgggtcgt
    15421 ggcaagaaaa ccggacggtc aggtccttga gttcgcttgc attgcaagga tcgatacacc
    15481 ggtggaactc gattactacc gtcacggcgg catactgcct ttcgttctgc ggagactctt
    15541 acaaagttag catccactcc aagggacgtg ttgcccgctg gcaattaacg tgtcctgtga
    15601 ccgcgtgtgc cgtcccattt ccgagcggct ttcctcgcaa tctgcacgaa gtgcggttcc
    15661 gcgacgcgac ggaagggtac cggctcgtgg tactcgaccg gatgattgta acggcggccg
    15721 gttgccgtaa tacatgtc
//
LOCUS       DAOSUT010000091        15403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2345_length_15403_cov_9.187777, whole genome shotgun sequence.
ACCESSION   DAOSUT010000091 DAOSUT010000000
VERSION     DAOSUT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_15403_cov_9.187777"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..2673)
                     /locus_tag="PLD05_09565"
     CDS             complement(37..2673)
                     /locus_tag="PLD05_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77729.1"
                     /translation="MTNHQRHEPTWPFLIILVILFILSATSPRAWRIVERPDPPRPKQ
                     KPELALASAAQRTPHTTKPHESTTERSPLSPIQKDHTQPAAESALGGSSSSVTSFQPD
                     GFSQSREGFSQLEVQQPIGNNGDQSEAPLGMREPTGDLVSELSGNYAQEMAEFAAESH
                     EPCHEAEAPQTVADATDAQAHDAVAKGNAWTVPESLLVLLDSLANVEALRPWVTTVRN
                     QVERLGTAFEQDSLEVALVLDELQRCIDRSSDLQMSLGEDSQLAGQFRQVRYALIRRL
                     ALWKSLHLAGGLNVELAKTHDADWRRISQKITALNARLGEGSTAQAWREFLNVEAVSE
                     IARAARANPSDCSKWESQLNQVARDSLARIGRQELTPEQHALLQEPALEDYLNELRFW
                     QGQSLTGAELLRVVEAFETRGLVSDGEAVLSHYYRLSRSSEEPSRWIGQCLNYLYRNA
                     NLRIVITADLLNRLLPQRPREYRPVSETILNRPVYGSSLAETTVAFRLIPDRQRARLA
                     LIVRGEVTSLTYAQAGPATVWNDGFGRYVAEKAIEITPRGLRAEPAVIDVQNDVRLRD
                     VSTSLDSVPVLGFLTQEIARDQVARRRLEANEEARWKLAAQALREIENETESRFEELN
                     ERLARDVLGPLYSLNMSPKWVAAETTNDRIILRIHLGSAMQPGGHTLRPFALSHSLAS
                     CQIHESTVNNFLAQLHLEGQTFSIAELQTKLREKLRLPDLDSPEDNPHAHAQITFAAK
                     DAVRVEFRDGRLNVLLSIARLVSSPNRWENFQVRAIYKPEISHDRIQFVRDGVIQLVG
                     PMDMRSQIALRGVFSKTFSKERPWQVSPGFLEKNPHLADLTVTELTLEHGWLGFSIGT
                     RSAQVNQTANRLPAPSSPPYGS"
     gene            3125..3667
                     /locus_tag="PLD05_09570"
     CDS             3125..3667
                     /locus_tag="PLD05_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77730.1"
                     /translation="MAGLGRFRLWYLLHLARPSHLRLLYRQVHAHPPRSLMEFGVEVR
                     RTLTLLQIICQMSGGRIPRYIALDEFESSSKKDPVSLKGLYRTLRRLGVEPRLLPGTP
                     EAVLPIWANHIRDVELLVIWKENQLPPDNRLWLFVPRMLAPDGQIWCETTSSGRHRWQ
                     VISKAEAERRAENAIRRRAA"
     gene            complement(3697..4359)
                     /locus_tag="PLD05_09575"
     CDS             complement(3697..4359)
                     /locus_tag="PLD05_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77731.1"
                     /translation="MSRNLPRTALLAGYYEDYLKHQDTARFVAEVNVRYTQGTLQRLL
                     EHPACEIRRAAALALGFLGNYDSNAALGKLLNDPDPTVRLLAETSIRKVWLRYGTESQ
                     QKQLSVIIRLNAARRYQEAITRADRLIEEAAGFAEAYNQRAVAYYGIGEHAEAIRDCR
                     MALELNPYHFAAAAGMGQAYLELRDPVAALDAFRRALKLNPDLQAVRMQVARLERLIE
                     DA"
     gene            complement(5095..5436)
                     /locus_tag="PLD05_09580"
     CDS             complement(5095..5436)
                     /locus_tag="PLD05_09580"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOP77732.1"
                     /translation="MLKSKIHRATLTATNLDYEGSIAIDRNLMEAADLLPGEQVHVLN
                     INNGERLITYVIEAPAGSGTVMLNGAAARLGMKGDLVIILSYCSIDDATARQYTPKVV
                     RVDGQNRLLAG"
     gene            complement(5652..6134)
                     /locus_tag="PLD05_09585"
     CDS             complement(5652..6134)
                     /locus_tag="PLD05_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77733.1"
                     /translation="MSYQNTQYGKWYWMTPLVLGTLLVIDLLVPGQPIPWFGYAIAGG
                     TVAMVTLCFTSLTVRDGGDKLVVQFGPIPLVRATIPYRSITRVEQGRTNFFDGWGIHY
                     SLTGGTVLNIWGFDCVRIWCGSRLYSIGTDDPDGLLTFLKNKLADLGSNTGITEDGSK
                     "
     gene            complement(6186..7253)
                     /gene="recA"
                     /locus_tag="PLD05_09590"
     CDS             complement(6186..7253)
                     /gene="recA"
                     /locus_tag="PLD05_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOP77734.1"
                     /translation="MAKKAGLKPEEKEAKKDSSTPLIEKNPALKNAVQQIEKQFGEGA
                     IMPLGASKVEAIEGIPTGSLSLDLALGGQGIPRGRIIEIFGPESSGKTTLALHVIAQA
                     QKLGGIAAFIDAEHALDPAWAKRLGVDLENLLVSQPSSGEEAMHIAEMLIRSNAVDVI
                     VIDSVAALVPQKELEGEIGDSHVGLQARLMSQSLRKLTGAIAKAKTAVIFINQIREKI
                     GVMFGSPETTPGGRALKFYASCRIDVRRVGQIRDGEQVIGQRVKARVVKNKVAPPFRV
                     AEFDMLHDSGISYEGDVLDLAIERKLVVRSGAWFRYGDLQLGQGRERTREYLKEHPEL
                     VEELRQRILETPGTIHTEIAE"
     gene            complement(7483..8562)
                     /gene="truD"
                     /locus_tag="PLD05_09595"
     CDS             complement(7483..8562)
                     /gene="truD"
                     /locus_tag="PLD05_09595"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOP77735.1"
                     /translation="MGTPEAVDEIRKQWRLPLHAVSYGGLKDRHAITRQYLTIRNGPS
                     SSLTLEGIRLEYLGQASRPFTSDDITANHFRIVIRDLTLEDMEKAITALQEVGQFGLP
                     NYFDKQRFGSVGWSGDFVARAWCLGDYERALWLALADPHDSDRSSDKKEKAYLREHWG
                     DWLACKQVVRNRFRRAVVAQLADQPGGYRRAMAALRRDQRGLYLAALQSYLWNVMASQ
                     WIVRHVPPENRFDVQIGRWAFAFFRRLSDPVLEQFRTTHLPLPSARCPVKTGPIRELM
                     EQAAAEVGLQVRQLRIKYPRDSFFSKGDRPLLVFPERLVSHTERDELNAGRQKLTLEF
                     DLPRGSYATILVKRITEDHGETDLL"
     gene            8902..9411
                     /locus_tag="PLD05_09600"
     CDS             8902..9411
                     /locus_tag="PLD05_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOP77736.1"
                     /translation="MASVVQDSQEALIQELSHHGGSGSMQLVSFRLGQEEYGVEITKV
                     QEIILMGEITRVPQTPEYIKGLINLRSTVIPIVDLRLRFGLMPQEPTDETRIMVVNVR
                     GKTIGIIVDAVSEVLRIHRDQIAPPPPTVAGLGQKYLTGLVKLDKRLLILLDIERILG
                     DDDTDFGGM"
     gene            9720..10733
                     /locus_tag="PLD05_09605"
     CDS             9720..10733
                     /locus_tag="PLD05_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOP77737.1"
                     /translation="MLFSRSDKSPSSPSNRSKGVLGDSPGLVPKIVPIEPIVSPLNLP
                     QVTDIELQRYADLIYRRTGVRISPQKKTLLSNRLRRRLKETGIRTYSDYYEYLTKLPP
                     NHPEWDAFLQEITTHETYLFRDEVQWRWFREKFLPEMAATQAGPIKTLRIWSAACSTG
                     DEAFTAACCIAAALPDFRSWRITILGTDIGVAAVEQAKTAVFGERAMRLVPEDYKKRF
                     FDKIPNAMVWRAKPILTGMTQFRQHNLMEPLRERPFDLVFLKNVLIYFDNASKQVVVK
                     NVLNVLRPNGYLVVGPAEGVGDLLRNMRRSQPWLYQKEPEQVTPAATSPAGSLLGATE
                     PRR"
     gene            complement(10779..11441)
                     /locus_tag="PLD05_09610"
     CDS             complement(10779..11441)
                     /locus_tag="PLD05_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77738.1"
                     /translation="MLVVTCPSCQARLKVSPQFVGRAGKCPRCGEIVTIPQAEPPQPV
                     TSEQANQASSGSDEDLPGIEHADRLVRTFHYLVCDRLGLVATWEANGQGWMIRSGTTM
                     VRAIKNADKLPAQGDFKLVELQIVPRTDGSHRLEGLHVYQLAPRYALTRIERGEDAIL
                     GAIRGPGSLSRDQKAAVFRYIREKFMREIWEDNQALVDYLTNQDYHSPGISPPTHPTF
                     PA"
     gene            12031..>15403
                     /locus_tag="PLD05_09615"
     CDS             12031..>15403
                     /locus_tag="PLD05_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOP77739.1"
                     /translation="MNKMERLPGSFFGPATLVELLRHRAKYQPDDIAFIYLVDGESEE
                     RPITYRELDRQARAIAAWLQTHDLVGERALLLYPPGLEFIAAFFGCLYGGVVAVPVYP
                     PRRNRSMERIEAISDNARAKVALTTRAVRERVDSLIHETPHLQQLTWLTTSEIEPGME
                     DRWQVPDISGETLAFLQYTSGSTGTPKGVMLTHANLMHNSALICYAFEHRRSGTGVYW
                     LPSYHDMGLIGGIIQPVFVGRPNVMMSPMAFLQKPYRWLAAITKYRGTTSGGPNFAYD
                     LCVRSITPEQRATLDLSSWEVAFNGAEPVRAETIERFSEYFAPCGFRKEAFYPCYGLA
                     ESTLMVSGGFVKEPPVIRFFDPEEIARGRAVICGPDNPRARPLVGSGRQLPDGRIVIA
                     DPETHRRLPDGQIGEIWVQSPSVAIGYWENEQATRETFHAHLADGDGPFMRTGDLGFL
                     WEGELFVTGRIKDMIIVHGVNVYPHDIEQTVQGADPRLRQNAGACFAVEQDGREQLVI
                     VQEVERRVKDGFDAIFQAIRRAVSLEHELAVDAIVLIKPGTIPRTSSNKIQRFACRQA
                     YLEGTLEVVASWKRGDTPDEWKEPALTASSVRQTGETGRISDVGTSGHSPDNGDDHGP
                     LAMDQLAARLATVSQTGPAWQAAGKRAGPNGEDSPDRMTKAASLASASGAAKPPLDRQ
                     QIVQAVLSEVRRVARDRAEGLTLDTAITEIGLDSLERMEILASLEERFGGRFPEEILP
                     ELETCAQVVEAVEKYLGGQEQERRVVIPPDQEIPAEFYDFDKFPEYLALQERLQILEA
                     SGLGNPFFTVHEGITNDRTIIGGREFINFSSYNYIGMSGDPEVIRAVKEAVERFGTSV
                     SASRLVSGEKTIHRELEQELAQFLGTEAAVVFVGGHATNETVIGHLMGPGDLILHDAL
                     SHNSILQGAILSGARRRPFPHNDWRAADRLLELFRREYRRVLIVIEGVYSMDGDIPDL
                     PHFIEVKKRHKALLMVDEAHSMGVLGETGRGVGEYYHVPREDVDIWMGTLSKSFGSCG
                     GYIAGKKSLVEYLKYTAPGFVFSVGLSPPNAAAALASLRLLKAEPQRVARLRERAALF
                     LRLAKERGFDTGASHDSPVVPIIIGNSLHCMFLSQA"
BASE COUNT         3376 a         4270 c         4357 g         3400 t
ORIGIN      
        1 taacactcct catgttagca gcatgaatga ttgatcttac gagccataag gcggtgatga
       61 aggggccgga agacggttgg ccgtttggtt aacctgagcc gagcgtgtgc cgattgagaa
      121 tcccagccag ccgtgttcga gcgtcaattc ggtgactgtc aaatcggcaa ggtgaggatt
      181 cttttcgagg aaccccggcg agacctgcca ggggcgttct ttggaaaacg ttttgctaaa
      241 aacgcctcgc aaggcaattt gtgatcgcat atccatcggg ccgacgagct gaataacccc
      301 gtcgcggacg aattggatac ggtcgtgact gatttccggt ttataaatgg cgcgaacctg
      361 gaagttctcc cagcggttgg gcgaactgac cagccgcgcg atcgatagaa gcacattgag
      421 gcgaccgtcg cggaactcga cacgaacggc atccttagcc gcaaaggtga tttgggcgtg
      481 cgcgtgggga ttgtcctcgg gcgaatcaag gtcgggcagt cgcagtttct cgcggagttt
      541 ggtttgcaac tcggcaatgg agaaggtttg cccttccaaa tgaagctggg cgaggaagtt
      601 gttcacagtg gactcgtgaa tttggcaact cgccaaggaa tgtgaaaggg caaaaggtcg
      661 cagtgtgtga ccgcctggtt gcatcgccga gccaaggtga attcgcagga ttatgcggtc
      721 gttggtcgtt tcggcagcca cccatttcgg cgacatgttc agactataaa gcggccccaa
      781 gacatcgcgg gccaatcgct cgttgagctc ttcaaaccgg ctttcggtct cgttttcgat
      841 ctcccgtaag gcctgggcag ccagcttcca cctggcttct tcgttggctt ctaggcggcg
      901 gcgggcgacc tgatcgcggg cgatctcctg cgtgagaaag cccaacacag gaactgaatc
      961 tagcgaagtt gacacatccc gcaggcgaac atcgttctga acgtcgatca cggccggttc
     1021 cgcacgtaac cctcgcggcg tgatttcaat cgccttttcg gccacataac gtccgaaacc
     1081 atcattccat accgtggccg gccctgcctg agcatacgtc agcgaggtca cctctccccg
     1141 gacgatgagg gccagtcttg cccgttggcg atcggggatc aggcggaagg ccacagtggt
     1201 ttctgcaagg ctggaaccat acaccggtcg attgaggata gtctcggaca caggccgata
     1261 ttcccgggga cgctgaggga gcaaacgatt gagcaaatcc gccgtgataa cgatgcggag
     1321 gttggcattt cgatagagat agttcaaaca ttgcccaatc cagcgagatg gttcctcgga
     1381 cgaccgtgat agccggtaat agtgactgag gaccgcttcg ccatcggaca caagaccgcg
     1441 cgtttcaaag gcctcgacga cccgaagcag ttcggccccc gtcaaacttt gcccttgcca
     1501 gaagcggagt tcgttgaggt agtcctccag ggcaggttcc tggagcaacg catgttgttc
     1561 gggggtcagt tcctggcgac caattcgggc aagtgaatcc cgcgcaacct gattaagctg
     1621 actctcccat ttcgagcaat cggagggatt cgccctagca gctcgcgcga tctcgctgac
     1681 ggcctccacg ttgaggaact ctcgccaggc ctgagcggtg cttccttcgc ccagccgagc
     1741 attcaaggct gtaatctttt gagaaattcg tcgccaatcc gcgtcgtggg tcttggccag
     1801 ttcaacgttc aggccgcctg ccaggtggag ggacttccaa agggcgagac ggcggattag
     1861 ggcgtacctc acttgcctaa attgccctgc aagttgggaa tcctcgccca agctcatttg
     1921 cagatcggac gatcggtcga tacatcgttg taattcgtcg aggaccaggg caacctcgag
     1981 agaatcctgc tcgaaggccg ttcccaatct ttcaacctga ttgcggacgg ttgtcaccca
     2041 cgggcgaagt gcctccacgt tggcaagcga gtccaacaac actaaaaggg actcgggaac
     2101 cgtccaggca tttccttttg cgacggcgtc gtgagcttgc gcgtcggttg cgtcggcaac
     2161 cgtttgcggt gcttcggcct catggcacgg ctcgtgtgac tcggcagcga attctgccat
     2221 ctcttgggcg taatttccgg acaactcgga gactaagtca cctgtgggct cccgcattcc
     2281 cagtggagct tcggactgat caccgttgtt gccaattggt tgctgcactt ccagttgaga
     2341 aaaaccctct cgggattggg aaaagccgtc tggttggaac gaggtgaccg aggaggaact
     2401 gccgccgagc gctgactccg ccgccggttg tgtgtgatct ttctgaatgg gtgaaagcgg
     2461 tgagcgctcc gtagtgcttt catggggttt tgtggtgtgt ggagtacgtt gcgcagcact
     2521 agccagcgcg agctctggtt tctgtttggg ccgaggtggg tctggacgct cgacaatccg
     2581 ccaagcccgg ggcgatgtgg cactgaggat gaaaagaatc acgagaataa tcaagaacgg
     2641 ccaggttggc tcatgccgct ggtgattcgt catgggtcct ttccttcgat ccgtgtccga
     2701 aaggctcctt gcgagaaggg cgatcggaac tcaaccggca aagaatccgt ctccgaggtc
     2761 caccaaaccg ggccaaaggc ccgtggtgtg gctgagtctc ccagatttcc cccctaaatg
     2821 gttttcgcct gttgaagcga ataactcaaa aacgctttgc gtaatcctcc tgagcacaaa
     2881 attcctatta gtttgcctat tttgacgctc gacaaatgca gagactctcc cgtgtctaaa
     2941 tcggcttgaa aggctgcccg cctccagcag cctgaccgga atccggtcac tcgggagcta
     3001 tcgcccaaag atgccccaca tagcgcaaag attgcctaga gttatgacgc caatcggccg
     3061 acatattcaa cacgttttgg atagcaacag aggtttgtgg aagtccatcg caaggaggga
     3121 actcttggcc gggttgggtc gattccgttt gtggtatctt ctccatcttg ctcgaccgtc
     3181 acatcttcgc ctcttgtatc gacaggttca tgctcatccg ccccgctcac tcatggaatt
     3241 tggtgttgag gtacggcgga cgctgacgtt gcttcagatc atttgtcaaa tgtcgggggg
     3301 aagaattccc cgatacatcg ctctggacga atttgaatcg tcgagtaaga aagaccctgt
     3361 cagtctgaag gggctctacc gaacgttgag aagacttggc gttgaaccac ggttactacc
     3421 gggcacgccc gaggctgttc ttcccatttg ggccaatcac atcagagatg tcgaactgtt
     3481 ggtgatttgg aaagagaatc agctaccgcc ggataaccgt ttgtggctct ttgtaccgcg
     3541 gatgttggca ccagatggcc aaatatggtg cgaaaccacg tcttctggaa ggcatcgctg
     3601 gcaggtgatc tcgaaagccg aagcggagcg acgggctgag aacgccataa gacgccgggc
     3661 tgcctgatcg ctcattgacc aacctgaggt cgggcctcaa gcatcttcga tgaggcgttc
     3721 taatcgggcc acctgcatcc gcacagcctg aagatcggga ttaagcttca aggcccgtcg
     3781 gaaggcatcc aatgccgcca ctgggtcgcg cagttccaga tacgcttggc ccatcccggc
     3841 agccgcggcg aagtgataag ggttcaactc aagagccatg cggcagtccc gaatagcttc
     3901 cgcgtgttcg cctatcccgt agtaggccac tgcccgttgg ttgtaggcct cggcaaatcc
     3961 ggcggcttcc tcgatgagtc ggtctgcccg cgtaatggct tcctgataac ggcgagccgc
     4021 attgagccga atgatcacac tcagttgctt ctgctgcgat tccgtaccgt agcggagcca
     4081 caccttgcgg atgcttgtct cggccaagag ccgaacggtg gggtcgggat cgttgagcaa
     4141 tttcccgagg gcggcattgg aatcgtaatt gccgagaaag cccagggcga gggcggctgc
     4201 ccggcgaatc tcacaagccg gatgctcaag caagcgctgg agcgtcccct gcgtgtaacg
     4261 gacattgacc tcggccacaa aacgagccgt atcttgatgc ttcaggtaat cttcgtagta
     4321 gcctgcaagt aaggccgtgc gaggcaaatt ccgggacacc gtcacactcc tgttgatcaa
     4381 aaacccggcc tcttcgacgc ggccaaccgc tcatcgagcg ctccagatcg caaggacaag
     4441 ccacattccg ttgccgtgcg tttggcacgc ggacaagtca tttgattcga ttgtaatcag
     4501 accctggcca aacgctcaaa cattttccat gggcaaagcg ctattgtcca cccggtggct
     4561 tggtcaggaa caggaaaaac aaccccaccc gggagggttc ggccatgagg acacatttga
     4621 gaagacactt gaggaaggaa acacggcttg ccggacgaaa ctttaaccgc ccaacttggt
     4681 gcgcaaggcg cgaggaaaca ctaggcggag cgccgttgtg tcagacgatt acttcttgcg
     4741 atgccgaatc ttggaccgca ttcggcgctt cttgctgcca atcttgcgac gccctttgcc
     4801 agttttcttt gtgcccacag gttactccaa tttcagaaca ccaacatatg ttcgaaaata
     4861 ccaacaacac ctcggcatga ggaaccatta aaggggaggt caaccacaga tagccttagt
     4921 ataactctca gttagaggcg gcgacaagga tgggcaccaa taccgcgccg aaggtccgca
     4981 ccgtcatggc gagagaatgc cccgtgcagt gatcagctta atcgtccgac gaaaatgagg
     5041 taatgggcca agatatgaaa cgattcacgc aaaaggccga cgatcgttcc ccaatcatcc
     5101 ggccaacagg cggttctggc catctacccg gacaaccttg ggcgtgtatt gccgcgccgt
     5161 ggcatcgtcg atgctgcaat aagagaggat gataaccaaa tcccccttca tacccaagcg
     5221 ggctgcggca ccgtttaaca tgaccgttcc ggaacctgct ggggcctcaa taacataggt
     5281 aataagtcgt tccccgttgt taatattcaa gacatgaacc tgctctccgg gcagcaggtc
     5341 cgctgcttcc attaaattac ggtcgatagc gatacttcct tcgtagtcca aattcgtcgc
     5401 ggtaagcgtg gcgcgatgaa ttttggactt aagcatgaat cgttgcacgc caaaaccccc
     5461 atctaagatc acacagtcgg caaacctgcc cgtactactt gggattccat ctcaccctga
     5521 cgccacataa gcgggcgaag ccatcccaac actttcattg taggcttctt cccgaaagaa
     5581 tgggcaggtc gccccgacgc gttctcgcag ctctgccaaa aaatgactcg gtcaaaccta
     5641 ctagaaaagg ctcattttga gccatcttca gtgatacctg tattacttcc cagatcggcc
     5701 aatttgttct ttagaaaggt aagcaaacca tcggggtcat ccgtaccgat gctgtaaagt
     5761 cggctgccac accagatgcg gacacaatcg aatccccaaa tgttgagcac cgtacccccc
     5821 gttagactgt agtgaatccc ccagccgtcg aagaagttgg ttcgaccttg ttcgacgcgg
     5881 gtaatggacc ggtagggaat tgttgcccga actaaaggaa ttggtccaaa ctgcacaacc
     5941 aatttgtccc cgccatcgcg gaccgtcagc gatgtaaaac atagcgttac catcgcgact
     6001 gtgccgcccg caatggcata accgaaccaa ggaatgggtt gccctggcac aaggagatcg
     6061 atgaccagaa gagtcccaag gaccaaggga gtcatccagt accacttgcc gtattgagtg
     6121 ttttgataac tcataaagga gaagtgattc caatcccggt cagggactcc aaagatcgct
     6181 ggagattact cggcgatttc cgtgtggatg gtccccggtg tctccaagat tcgctgccgc
     6241 agctcttcca caagctctgg gtgctctttg aggtattcgc gggtacgctc tcgcccttga
     6301 ccaagttgaa ggtcaccata ccggaaccac gccccgctgc ggacgacaag ctttctctcg
     6361 atggccaggt ccagaacgtc accctcataa ctaatgccgg aatcgtgcag catatcgaat
     6421 tctgccacac ggaacggcgg cgccaccttg tttttgacga cgcgggcttt gacacgttga
     6481 ccgatgacct gttcgccgtc gcgaatttga cccactcggc ggacatcgat ccggcacgag
     6541 gcatagaact taagtgcccg gcccccgggg gtggtttcgg gcgatccaaa catgaccccg
     6601 attttttccc gaatttgatt gataaaaata acagcggtct tggctttggc gatcgcccct
     6661 gtcagctttc gcaatgattg gctcatcagt cgggcctgta atccgacgtg agaatcaccg
     6721 atttcccctt caagctcctt ttgaggaacc aaggcagcca ccgagtcgat cacgatgaca
     6781 tcgacggcgt tagaacggat gagcatctcc gcgatgtgca tcgcttcttc gccactggat
     6841 ggctgactga cgaggagatt ctcaagatca acgcccaacc gcttggccca ggccgggtca
     6901 agggcatgct ctgcatcgat gaaagcggca attcccccca acttttgcgc ttgggcgata
     6961 acatgcaagg cgagagtggt ctttccgctt gattcgggac caaaaatctc gatgatgcgt
     7021 ccgcggggga ttccctgccc accgagtgcc aaatccaagg aaaggctgcc agtgggaatc
     7081 ccttcaatgg cctccacctt gctggcccct aacggcataa tggccccctc tccgaactgc
     7141 ttttcgattt gctgaaccgc gtttttaagt gccgggttct tctcgataag aggggtcgaa
     7201 gagtcctttt tcgcttcctt ttcttcgggt tttagccccg ctttcttggc catgaggtca
     7261 acctccggtt gttcgctgtc aatcgttctc gataaagaac tgatctcatt tccgcgatca
     7321 gaagaagtct cggtagaccg gttctgcgac gattcttctc gtccgcgctc agtatagcgg
     7381 tttgcgcggc ccagtcacaa gatacgggta gcgaggccgc gcatcctttg tgttttcttt
     7441 ctcgtcacaa aatgggctgc cgctgaggga gagaccgcta ccctaaagca ggtctgtttc
     7501 gccgtgatct tctgtgatcc gtttgaccaa aatggtcgcg taggaacccc ttggaagatc
     7561 gaattccaga gttaactttt ggcggccagc gttcagctcg tcgcgctcgg tgtggctaac
     7621 cagtcgttca ggaaacacga gaaggggacg atcacccttt gaaaagaagc tatcccgcgg
     7681 gtacttgatg cgcagttggc ggacttgtag cccaacttcc gcggcggcct gttccatcag
     7741 ttctcgaatg gggccggttt taacgggaca acgtgcggag ggaagtggta aatgcgtggt
     7801 tcggaattgc tccagcacag ggtcggagag ccgacggaaa aaggcaaacg cccatcgccc
     7861 gatttggacg tcaaatctgt tctccggggg gacatggcgg acaatccact gcgatgccat
     7921 gacgttccag agataacttt ggagggctgc caggtacaat ccccgttgat cacgtcgcaa
     7981 ggcggccatg gcccggcgat acccacccgg ttggtcggcc agttgggcca cgacagcccg
     8041 gcggaatcgg tttcgcacca cctgcttgca cgccagccaa tcaccccagt gttcccgcag
     8101 gtacgccttt tcctttttgt cactggaacg atccgagtcg tgcgggtcgg ccagggctag
     8161 ccacaatgcc cgctcgtagt cgccaagaca ccatgccctg gccacgaaat cgccggacca
     8221 tcccaccgag ccaaatcgtt gcttatcgaa gtagttgggt aaaccgaatt ggccaacctc
     8281 ctggagggcc gtgatggcct tctccatatc ctccagagtc aaatcccgaa tcacgatccg
     8341 gaagtggttg gccgtgatat catccgaagt gaacgggcga gaggcttggc ctagatattc
     8401 caaacgaatt ccttctaagg tcagcgacga gcttgggcca ttgcggatgg tcaggtactg
     8461 ccgtgtgata gcgtggcgat ccttcaatcc gccgtaagag accgcgtgca ggggaagtcg
     8521 ccactgtttg cggatttcat cgacggcctc cggcgtgccc agcgattact tggtcagacg
     8581 atacaccgcg taggggccgt tatccaaggg aaaatcagtg atttcctcga cgataaagtc
     8641 agccggtatt tgtttgagtt tcatgaggaa aacacgccat tgctgattgg gaagctcgtg
     8701 tcgtttgtgc gttttgtagg gacaaggggt ttaacccctg tgtttgcgat tttttctcaa
     8761 agtgttgcct gaagaaaggc atcatgggaa tccataacct atagtttcat caaagcggct
     8821 cgtgggatat tcgtctagaa gccgccgttt gaggtgcaaa aaacggttgg aaaaatgttc
     8881 ctgaaccagg aggtaccacc catggcaagt gtcgttcaag attctcaaga ggcccttatc
     8941 caagagttgt cccatcatgg cggcagcggc agcatgcaac tggttagctt ccgtttggga
     9001 caagaggaat acggcgttga gatcaccaag gttcaagaga tcatcctcat gggtgaaatc
     9061 acccgggtac cgcaaacgcc ggagtacatc aagggattga tcaatcttcg cagcacggtg
     9121 attccaattg tggatttacg gctccgcttt gggttgatgc cccaggaacc gacggacgag
     9181 acacggatta tggtggtgaa cgtccgcggc aagacgatcg gtatcatcgt tgatgcagtc
     9241 agcgaggtcc ttcggattca tcgagaccag atcgctcctc cgccgccgac cgttgccggc
     9301 ttgggccaga aatacctgac tggcttggtc aaactggaca agcggcttct cattctgttg
     9361 gacatcgaac gcattcttgg cgacgacgac accgatttcg gtggaatgta atcgcactcg
     9421 tagtgaggcg ggacgcctct aagccgcctg atggtttact cattgactcg gcccacagcc
     9481 gcagccggga acgccccacc acagtgaagg gttgcagagg ccaagacagt ccgccgcgct
     9541 caaaagagcc tctcggggcg cagcggctgt ggcgccgaga gttgaagctg cggataggaa
     9601 cgctcggtag cgttgccact cggccggact gcggtcggtg gactcggatg gccaaacatt
     9661 cctggcatca gtttccagtg tgcgagtgag ttttgatccc ctgtgaggaa tcgggaacga
     9721 tgctgttttc tcggtcggac aaaagcccaa gctcaccgtc aaatcgatcc aaaggtgtgt
     9781 tgggagattc accagggttg gttcccaaga tcgtgccgat tgagccgatt gtcagtcccc
     9841 tgaaccttcc ccaggtaact gacatcgaac tgcagcggta cgcggactta atctatcgcc
     9901 gcaccggcgt tcgtatctcg ccgcagaaga agacattact ctccaaccgt ctcaggaggc
     9961 ggctcaaaga gaccggaatc cgcacctata gcgattatta cgagtacctc accaaacttc
    10021 ccccgaatca cccagaatgg gacgccttct tgcaggaaat caccacccac gaaacgtacc
    10081 tctttcgaga tgaggtccag tggcgatggt ttcgcgaaaa attcctgccc gaaatggctg
    10141 ccacccaagc gggaccaatc aagacgttgc gaatttggtc ggcggcttgt agcacgggcg
    10201 atgaggcctt cacggccgcc tgttgcatcg ccgcagcgtt gcccgatttc cgttcctggc
    10261 ggattaccat tttagggacg gatatcggtg tcgcggccgt ggagcaggcc aagacggctg
    10321 ttttcggcga aagggccatg cggctggtcc ccgaggacta caagaaacga ttcttcgaca
    10381 agattcccaa cgccatggtg tggcgggcta agcccattct gaccggcatg acgcagttcc
    10441 gccagcacaa tctgatggag cctttgcgag agcgcccatt cgacctcgtc ttcttgaaga
    10501 acgtgctcat ttactttgac aacgcatcca agcaggttgt tgtcaagaat gttttgaacg
    10561 tactccgccc caacggttac ctcgtcgttg gacctgcgga gggagtgggc gatctgctac
    10621 gtaatatgag gcgatcgcag ccgtggcttt accaaaagga accagaacag gtaacccccg
    10681 cggcaacctc acccgcaggt agtctcttgg gagccacgga gccccgtcgt taacaagaca
    10741 gcacaccccg cgcgaccgat cgagaacaca tttgaccttc atgcgggaaa ggtgggatgc
    10801 gtcggcggcg aaatcccggg ggaatggtaa tcttgattgg tcaaataatc caccagggct
    10861 tgattgtcct cccaaatttc ccgcatgaac ttttcccgga tatatcgaaa cactgccgcc
    10921 ttttgatccc ggctgaggct cccgggacca cgaatcgcac ccaggatggc gtcctctccc
    10981 cgttcgatgc gggtcaaagc gtagcgcggg gccaactgat aaacgtggag tccctccagc
    11041 cggtgggaac catccgtacg gggcacgatt tgcaattcca ccagcttgaa atcgccttgg
    11101 gcagggagct tatcggcgtt ttttatggct cggaccatgg ttgttccgga acggatcatc
    11161 caaccttgcc cgttagcttc ccaggtggca accaagccga gacggtcaca caccaggtag
    11221 tgaaacgttc gcacaagtct atcagcatgt tcgatgccgg gaagatcttc gtctgatcct
    11281 gaagacgctt gattagcttg ctccgatgtg acaggttggg ggggctccgc ttgaggtatg
    11341 gtgacgattt ctccgcaccg tgggcatttt cccgcgcgac ccacgaattg cggactaacc
    11401 ttcaatcgag cttgacacga ggggcaagtc acgacgagca tctgttatcc tcccgttgga
    11461 gtgattccgg cagcgccatt ctgctttcat ggtccgtcgg ggttggcgaa atttcaacca
    11521 tggctcacct aagatacgta tacaacccca ctacgttggg gtgtattccc cgcctttgcg
    11581 tccccaatcg caaaagcgta acatgagagg ataggcaaag aggaatagac tcaagcggtt
    11641 ggggaagaat acccgtagaa aatgggtgag tcttccgaac ggattgttcc aggggataca
    11701 ttgcgctcag agtctaaaca gggggtggtt gatgggggca gccggagggg gtaacgcgtg
    11761 gtgatccgtg taacatgatc cttgggcgct agcagggata atcgcccggt tgtgtcttga
    11821 ggatcttggc ttcatggatt gtcatcatgc aaggaatcca ttgctaagtg cgtcgaagaa
    11881 cctagtggtt tgctgagagt taatcgccga tatcgagtgc gttaacaaga ttgcgggcct
    11941 cccgttagaa tgtccttcac cacgttcgta cccagaacgc accgttagat taatcatcac
    12001 ttggcgtcag tcgataggta gctgaggttc atgaataaga tggagcgatt gccaggtagt
    12061 ttttttgggc cagccacttt ggtggagttg ctccggcatc gggctaaata ccaaccggac
    12121 gacatcgctt ttatctacct tgttgatggt gaaagcgagg agcgaccgat tacctatcgc
    12181 gagttagacc gacaggcacg agccattgcc gcctggcttc aaactcacga cttagtgggc
    12241 gaacgcgcct tattacttta tccccctggc ctcgagttca tcgccgcgtt cttcggttgc
    12301 ctgtacggtg gtgtggttgc cgtgccagtc tatcctccgc ggcgaaaccg ctccatggag
    12361 cgaatcgaag cgatctcgga caacgcgcgg gctaaggtgg ccttgacgac acgggcggtc
    12421 cgcgagcggg ttgacagctt aatccacgaa acgccccacc ttcagcaatt gacctggctg
    12481 accacgagcg agatcgagcc ggggatggaa gaccgctggc aggtgcccga tatttcgggc
    12541 gagacgctcg cctttttgca atacacctcg ggatcaacgg gaaccccgaa gggcgttatg
    12601 ctgacgcacg ccaaccttat gcacaactcg gccctcatct gttacgcctt tgaacatcga
    12661 cgatcaggaa cgggcgttta ctggctcccc agttatcacg acatgggtct cattggcggg
    12721 atcatccagc ccgttttcgt gggacgcccc aacgtcatga tgtctcccat ggccttcttg
    12781 cagaagccct atcgttggtt ggccgccatt accaagtacc ggggaaccac cagtggaggc
    12841 ccgaattttg cttatgattt atgcgtgcga tccatcacgc cggagcaacg agcgactctc
    12901 gatcttagct cttgggaagt tgccttcaat ggggccgagc cggttcgggc agagacaatc
    12961 gaaaggttca gcgaatactt cgccccgtgt ggcttccgga aggaggcctt ctacccgtgc
    13021 tatggtttgg ccgaatccac gttgatggtc tccggtggat tcgtgaagga acctcccgtg
    13081 attcgcttct ttgaccccga ggagattgcc cgggggcgtg ccgtgatctg tggccccgac
    13141 aacccccgcg ctcgcccgtt ggtaggatcg ggacgacaac tcccggatgg gcgaatcgtc
    13201 attgccgatc cggagacgca tcgtcggctg ccagatgggc agatcggcga aatctgggtg
    13261 caaagtcctt ctgttgccat tggctattgg gaaaatgagc aagccacgcg agaaaccttc
    13321 cacgcccatt tggccgatgg cgacggaccg ttcatgcgaa caggcgattt gggattcctt
    13381 tgggagggag agcttttcgt caccggccgc atcaaggata tgatcatcgt ccacggggtg
    13441 aacgtttatc cccacgacat tgagcagacg gtccaggggg ctgatccgcg attgcggcaa
    13501 aatgccggtg cctgctttgc cgttgagcaa gacggccggg agcaactcgt catcgtccaa
    13561 gaggtcgaac gccgagtcaa agatggattt gacgccatct tccaggccat caggcgagcc
    13621 gtctccctgg aacatgagtt ggcggtcgat gcgatcgtgc tgatcaaacc cggtaccatc
    13681 ccccgcacgt ccagcaacaa gattcagcga ttcgcctgcc ggcaggccta tctggaaggg
    13741 acgctcgagg tcgtcgcaag ttggaagcgc ggtgacacac ctgatgaatg gaaagagcct
    13801 gcgctaacgg ctagctcggt tcgccaaacc ggagaaactg gcaggatatc cgatgttggc
    13861 acctcaggac attcgccgga caacggggac gatcacggtc ccttggcgat ggaccaattg
    13921 gcggcacgct tggccacggt gagtcaaacg ggcccggcct ggcaggccgc cggtaaacga
    13981 gcagggccga acggcgaaga ctcgcccgat cgaatgacca aggcggcttc gcttgcatca
    14041 gcgtcaggcg cggcgaaacc acctctcgat cggcagcaga tcgtgcaagc ggttctttcc
    14101 gaggttcgtc gtgttgctcg cgaccgtgcc gaaggcttga cccttgacac ggccatcacg
    14161 gaaataggtc tcgattcgct ggaacgcatg gagattttgg cgtccctgga agaacgattc
    14221 ggtgggcgat ttcccgaaga aatcctccct gagctggaaa cctgcgccca agttgtcgag
    14281 gcggtggaaa agtatctcgg tggtcaggag caggaacgcc gtgttgtgat tcctcccgac
    14341 caagagattc cagcagagtt ttacgatttc gataagtttc ccgaatatct tgccctccag
    14401 gaacggcttc aaatcctgga ggccagtggc ctcggcaatc cctttttcac agtgcatgaa
    14461 gggatcacca atgatcgcac gattatcggc ggtcgggaat tcatcaattt ctccagttac
    14521 aactacatcg gcatgtcggg tgacccggag gtgattcgag cggtcaaaga ggccgttgaa
    14581 cgctttggga caagcgtctc cgccagccgt ttggtgtcag gggagaagac aatccatcgt
    14641 gagctagaac aggaacttgc acagtttttg ggcaccgaag cagccgtggt ctttgtcggt
    14701 ggtcacgcca ccaacgagac tgtgatcggt catttgatgg gaccgggcga cctgattctc
    14761 catgacgcgc tttcccacaa cagcattctt caaggggcga tcctctccgg tgcccgtcgc
    14821 cgtcctttcc cccacaacga ttggcgggcg gctgaccggc tgttggagct tttccggcgc
    14881 gagtaccgcc gtgtgctgat cgtcatcgag ggggtttata gcatggacgg ggacatcccg
    14941 gatttgcctc atttcatcga ggtcaagaaa cggcacaagg cccttcttat ggttgatgag
    15001 gcccattcca tgggggtcct cggagagaca ggccgcggag tcggcgagta ttaccacgtt
    15061 ccccgcgagg acgtggatat ctggatgggg acgcttagca agtcctttgg tagttgcggg
    15121 ggctacattg ccggaaagaa gtcgttggtt gaatatctca aatacacagc acccggtttt
    15181 gtctttagtg ttgggctttc ccctcccaat gccgctgccg ccttggcatc acttcgccta
    15241 ttaaaggcag aaccgcagcg ggtggcgcga ttgcgagaac gggcagccct cttcctccgc
    15301 ttggccaaag aacgcggatt cgataccggg gccagccatg attcgcccgt cgtcccgatc
    15361 atcatcggca actcgctcca ctgtatgttc ctttcgcagg cct
//
LOCUS       DAOSUT010000092        15396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2348_length_15396_cov_9.729092, whole genome shotgun sequence.
ACCESSION   DAOSUT010000092 DAOSUT010000000
VERSION     DAOSUT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_15396_cov_9.729092"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1660)
                     /gene="ffh"
                     /locus_tag="PLD05_09620"
     CDS             complement(182..1660)
                     /gene="ffh"
                     /locus_tag="PLD05_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOP77740.1"
                     /translation="MFEKLQSGLFEAIRTLTGKGKLTEANMREGLRMVQQALLEADVS
                     LPVVKAFLEKVSQEALGARVLQSLRPTHQLLAIVYEQLVALMGPVDHELRLKPSGATI
                     MLCGLQGSGKTTTAAKLGRYLQMKGRKPLLVAADLQRPAAIEQLQVLGEKIGVPVYVE
                     KGATDPLPVCRNALRQMSALGADVAILDTAGRLHIDEALMQQLAIIDRQIQPDEVFLV
                     VDAMTGQDAVNSAKAFHEALKLNGVILTKLDGDARGGAALSVKYVTGVPIKFIGTGEG
                     TDALEEFHPDRIASRILGQGDLMTLIEQAQQKIDQEEMRRQQERLQQGQFTLEDFRNL
                     LDQMRRLGPINQMMSLIPGMSQLVNEMDDVDAEHSLRRLRGIIDSMTPEERRKPEIID
                     LSRRRRIAAGAGVAPHDVAELVRVFEPIADIMRKLAGQGMRGRVQMLQTLMRQAATNP
                     EAMLSKPKVGTGKRLRPEEKARLKKLREKELRRRRKEQRRGG"
     gene            complement(2028..2762)
                     /gene="aat"
                     /locus_tag="PLD05_09625"
     CDS             complement(2028..2762)
                     /gene="aat"
                     /locus_tag="PLD05_09625"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOP77741.1"
                     /translation="MSAFGSSVLPPSRFFPSPEWADEHGIVAVGGTLHPDLLLDAYSH
                     GIFPWPVDEESPIVWASPEIRAIFDWDKIHIPRRLVPTIKRDGFKVTLDQDFRSVIRG
                     CATAPGRRGNTWITPAMIRAYIRFHELGFAHSVEVWQGEKLVGGVYGVALGGLFAAES
                     MFYRVSNASKVGLLYLLAHLRQRGYTLVDIQMLTPVTESLGAVEIHRREYLRRLASAL
                     KQPVTFGDILAVGPQQVLALDHRWRE"
     gene            3199..5061
                     /locus_tag="PLD05_09630"
     CDS             3199..5061
                     /locus_tag="PLD05_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOP77742.1"
                     /translation="MLNLVSSCDPKAGSFWACTLACLVFGISSYFVASSAAETKVGNV
                     LEETVLLDFTASWCGPCQRMAPLVDQLIGMGYPVRKVDIDREPALAKRFGIQAVPTFV
                     MLVNGREAKRIVGGAGLDDLIALCRLAPRAGSPSDSQSVVLTSATSSGVSPASTGVFP
                     LRGAALSDKAGARPAAIPETIPPLDENCRFAAANEQDLIAVTARIRVEDPHGRSTGSG
                     TIIDARGGEALIVTCGHLFRDSQGKTPILVDLFGPNAAQGIPGQLIYYDDRLDIGFIS
                     IRPNRPVVVARVAAPGYPLAVGDAVFSVGCTASQPPSVWEGRITALNKYLGPANIEAS
                     RAPFVGRSGGGLFSNEGFLIGVCNAADPPLDEGIYLALQEIHQSLDQLNLAFVYRWDA
                     ASRGMTGDPQVAQNRGQLAVSGPGGGSSGRGETENRFGQPQGPSTGLSPESLTLATGL
                     PLPIPMGDLSGSRTAQAMGTLGVPVPSGGMPSATVVPVGFVEHATLSLEEQATLEEIR
                     RRQAQGHQVVCIVQPADQPNSTSEIIVVRSPSPQLLAALSQAGVKMVMPGDLPSRTVG
                     EIPSDPFSARLGQQASDSPLVPIPAGAVLRIGTNVMPVSGRAVAGTRPGTAGWD"
     gene            5472..5762
                     /gene="gatC"
                     /locus_tag="PLD05_09635"
     CDS             5472..5762
                     /gene="gatC"
                     /locus_tag="PLD05_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOP77743.1"
                     /translation="MISLAEIEKVAVLARLLLTPAESQQMTEQLGKILEYMSLLNEVN
                     TENVEPLAHAVELTNVFREDRVTPSLDREMALANAPHRDEECYRVPAVLGEV"
     gene            5817..7379
                     /locus_tag="PLD05_09640"
     CDS             5817..7379
                     /locus_tag="PLD05_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOP77744.1"
                     /translation="MSLSELTASQLLQQLREGRIKAVEVVAHSLRQIDQYDPTVGAFL
                     RVNRDHAFAQAEEIDRRRSRGEKLGKLAGLPVAVKDNICTQGEPTTCASRMLKRFVPP
                     YDATVIAKLKAADAIIVGKTNLDEFAMGGSTETSALGRTCNPWDVTRIPGGSSGGAAA
                     CVAARMVPLSVGSDTGGSIRQPAALCGVTGLKPTYGRVSRFGLVAFASSLDQIGPLAQ
                     TAEDCALLLEVLAGHDPKDATSIPQPVPPFSETVRQPLEGLRVGWVREHLGEGVSPSV
                     LAAIQEAIKVFQSLGAKVEEVELPHARYAVATYYIIACSEASSNLARYDGVHYGYRTD
                     EKAMLAELAEERRVLQQQGKSEALEDLDTALVRMYRKTRAEGFGPEVKRRIMLGTYAL
                     SAGYYDAYYLKALKVRRLIRQDYDKAFQKVHVIVGPVTPTPAFALGEKINDPLAMYLF
                     DLFTVGTNLAGLPAISIPCGFSEDGMPIGLHLQAPPLEEETLLRAAHMFQQVTDWHRR
                     RPPLLDRQAVAT"
     gene            7458..8936
                     /gene="gatB"
                     /locus_tag="PLD05_09645"
     CDS             7458..8936
                     /gene="gatB"
                     /locus_tag="PLD05_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOP77745.1"
                     /translation="MAEQPYTIVIGLEVHVQLLTESKLFCGCSTKFGAPPNTQTCPIC
                     IGMPGVLPVMNRRAVELAVKTGLALNCEIARFSKWDRKNYYYPDLPKGFQISQYDLPI
                     CRGGYLDVPSPNREGMRRIRIIRVHLEEDAGKSMHDEKAGRADSRIDLNRAGTPLLEI
                     VTQPDIRSPAEAKAFLTELKLLLNYLEVSDCNMQEGSLRVDANINLHIPTEDGQYVAT
                     PIVEVKNMNSFRAVERALTYEAQRQYEAFLETGHRLGDVPKQTRGWDEDAQLTRPQRS
                     KEESSDYRYFPEPDLVPVTVTDAELEALRQQLGELPADIRRRLQETYHLNDYDSDVIV
                     NQGRAFVDYFCQLADTCGDGKMAANWAQQDVLRTLNERDWTITQFPISAAELARLLCR
                     VKSGELDTAKARMVFGHMVDHQATVDEAIQSLGIVQVDETELRQLCAELLASNPKVVA
                     EVKSGKDKAIGSLVGQAKKRNPHVNPARVKEICLELIRQETT"
     gene            9034..9813
                     /locus_tag="PLD05_09650"
     CDS             9034..9813
                     /locus_tag="PLD05_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOP77746.1"
                     /translation="MTQIVTLITDFGWESVYVAQMKAVLLSINRRLTIVDVTHEIPPQ
                     DVQAGALVLRHVVPSFPPGSCHVAVVDPGVGTQRPCLCADMGGRYVVAPDNGLITYLA
                     EVLPVNVMVRLENPAYRLPEVSATFHGRDVMAPAAAWLSLGVTLEQLGPVHHAPVLLP
                     EGWKPLVTERQIRGKVVLVDRFGNLITNIEKTHLSPDRTVTVSIGEYVIPGLVRTYGH
                     RTSGEVVALINSQGYVEVAVVNGSAAEVTGLGVGTPVVITW"
     gene            9820..11181
                     /gene="bioA"
                     /locus_tag="PLD05_09655"
     CDS             9820..11181
                     /gene="bioA"
                     /locus_tag="PLD05_09655"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOP77747.1"
                     /translation="MLDEREKQQLRQWDRTIYWHAFTQMAEYEPFIIERAEGCRLIDI
                     DGREYIDGVASLWCNVHGHRHPKLDAAIREQLDRVAHVTSLGCSNPTTIRLAKRLTEI
                     APPGLRHVFFADSGAGAVEVALKVAFQYWRQIPRPQPQKTVFLALGDAYHGDTLGAVS
                     VGGVDRFNAVFQPLMFPTIRLPCPETYRTPEGVPPSELTEYYLNQVEQVLRREHERIV
                     AMIIEPLVQCAAGIVMHPLGYLRGVRELTRKYNVLLIADEVAVGFGRTGRMFACEHEG
                     VTPDLLCLGKGITGGYLPVAATLVTDEVYNAFLGDYASLRTFFHGHTYAGNPLGAAVA
                     LANLDVFEEERTLENLPPKIERLRAHLERIANRPHVGHVRQCGLIAGIELVQDKATKK
                     PYPWTERRGWQVCLAVREEGIFLRPLGDVIVIFPPLAISLEEIDQIGFAVERAIIRVT
                     EQD"
     gene            complement(11348..12733)
                     /locus_tag="PLD05_09660"
     CDS             complement(11348..12733)
                     /locus_tag="PLD05_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOP77748.1"
                     /translation="MSRHDDVPFAAELPESLKINRRHFIGGSTALAGIAGARLGAWAA
                     EVSADQPVPLKPIGQGKGIFPGRVVWVHDPGVVDWKGPDDGRWWEGDHVKQSRVNAMM
                     DQAVRHLTGADSVKDAWHRLFSHLNKTRKGAAQGYQAGQKILIKPNWVGMIYRENVVD
                     PETYRFIRRENYMNTSPQMLLALLRQLVDEVGVKPADITICDTLAYLVHEYYDILHAH
                     YPDVQYVDYAGKFGRIKVQASETQLYWSCRPQGVAQDFLPRCFAEADYLINFANFKAH
                     SGAGVTLCAKNHYGSLIRWPVQEGYYDIHPNCFSTATGIYRPLVDLIGHAHLGGKTVL
                     YLIDGLFSGVHPRDPVPQRMHIPPMEDQWSCSLFASQDPVAIDSVAFDFLYAEGTPFP
                     RKGGVDDYLHEAALADQPPSGTFYDPNHAEPTQRLASLGVHEHWNNVKEKKYSRNLGA
                     SEGIELLPVTL"
     gene            13507..14259
                     /locus_tag="PLD05_09665"
     CDS             13507..14259
                     /locus_tag="PLD05_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77749.1"
                     /translation="MRLRKIDIRLEGPDIQPESIPVGELLDLLLCYERLLRDLTGKRH
                     SKVERKHREMRLRLVSVERGSTTLSCLAPEELYNANRTISQAIGKGSDLPPEARDSFF
                     RLRKRIEKLGCDVKIQGEDLEPFAISRERVPTKRFIRGETTVYGECIAVGGRKAVVKL
                     VSGSGDQIRIRLSNELAREIAKHLYEEIGVRGLATWEVSGPRIVDMEAYELLPYRRTS
                     PKEAIQALQESAGKDWGKIADPEEFLRKLRRE"
     gene            14273..14770
                     /locus_tag="PLD05_09670"
     CDS             14273..14770
                     /locus_tag="PLD05_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOP77750.1"
                     /translation="MGKRRSVILDTNILVYAIRPDDCNDDRAKRAKDFISDISRDDEA
                     DILIPAVVVAEFLCGCDSGFEDAVRNLQEHFKIVPFDMNAACQYRQVVTTRRRRSGSD
                     QDETKQVAAAERNTVDEMIVATALAARAQELITYDQGLINLAKAVGIDAKEPPELGLG
                     LLPGF"
BASE COUNT         3276 a         4154 c         4651 g         3315 t
ORIGIN      
        1 actctttata tgacgactct gactttccag gttcaacctt gaggggaacg gccgaacacc
       61 caggggaagc gtgccatcat cttggcaaaa gcaaaaagtc aggagagggg caaggggcca
      121 cgaaacgcgg cccacggtgt gtgggatttg tgattgaaga aagaatcaga aagagggcgg
      181 cttatccgcc gcggcgctgt tccttgcgtc gtcggcggag ttctttttca cggagctttt
      241 tcagtcgggc cttctcttcg ggacgcaggc gttttcccgt gccgactttt ggcttggaga
      301 gcatcgcttc cggattcgta gcggcctgtc gcatgagcgt ttgcagcatt tgcacgcgtc
      361 cacgcatccc ttgaccggcc agttttcgca tgatgtcggc aatgggctca aagacccgga
      421 caagctctgc cacgtcatga ggggcgacac ccgccccggc agcgatccgg cgacgccgac
      481 tcaggtcgat gatctccggc ttgcggcgtt cctcgggcgt catggaatca atgatacccc
      541 gaaggcgtcg caaggagtgt tcggcatcaa cgtcgtccat ttcgttgacc aactggctca
      601 tgccggggat gagtgacatc atctggttga taggaccgag gcgacgcatc tgatcgagga
      661 ggttgcggaa gtcctcgagg gtgaattgcc cttgctgcaa gcgctcttgt tggcgccgca
      721 tttcctcctg atcgatcttt tgctgcgcct gttcaatgag cgtcatgaga tcgccctggc
      781 ccagaatccg gctggcgatc cgatccgggt gaaactcctc gagggcgtcc gtcccttcgc
      841 cggtcccaat gaacttgatc ggcacgcccg tcacgtactt gaccgagagg gccgcaccac
      901 cgcgggcatc gccgtcaagc ttggtcaaaa tgaccccgtt gagcttgagt gcctcatgga
      961 acgccttggc gctgttgacg gcgtcctggc ctgtcatcgc gtccaccacc aggaacacct
     1021 catcgggctg gatctggcgg tcgatgatgg ccaactgttg catgagggcc tcgtcgatgt
     1081 gaagacgacc agccgtgtcg aggatcgcca catcggcccc cagtgccgac atttgacgca
     1141 gcgcatttcg gcaaaccggg aggggatcag tagcaccttt ttccacatag accgggacac
     1201 cgatcttttc cccaagtact tgaagctgct cgatggcggc gggtctttga aggtcggctg
     1261 ccaccaaaag gggtttgcgg cccttcatct gaaggtatcg gcccaacttg gcggccgtcg
     1321 tcgttttacc tgatccctga agcccgcaga gcatgatcgt ggccccgctg ggcttgagcc
     1381 gcagctcgtg atccaccggg cccatcaggg cgacaagctg ttcataaaca atcgccagga
     1441 gttggtgcgt tggacggagc gactgaagga cccgtgcgcc aagggcctct tgggagacct
     1501 tctccaggaa ggctttgacc acaggcagac tgacatccgc ctcgaggagg gcctgctgga
     1561 ccatccgcag cccctcccgc atgttcgcct cggtcaattt ccccttgccg gtaagtgtgc
     1621 ggatggcctc gaagagtcct gattgaagtt tctcaaacat ggagtgttac ctgaaccgga
     1681 gaacaaaacc gagcttgtac gcctgttacc gcgtcgctcc gccccaacgt tctacgagcg
     1741 tcttggaaac ctaagaagtg gcgctgccat cgggcgaaaa ccttctgtga ccgcctgcgg
     1801 catcatttca ccatcttagt cgtccccacc gtgttttggc aatgcaaacg gcgtcgtttg
     1861 agcgggtccc agccatgcga ttatggaaat ggtctgccgg tttgctggga caagccggtc
     1921 gcctaatgtg gagaaaagcg cgacatcgcg tccattgaga gaatatagcg tcgtattcca
     1981 cgacgacaag ccggcgcgca ctgccgggtc aatagattcg gccaggttta ttcacgccac
     2041 cggtggtcaa gggctaacac ctgttggggg cctacagcca ggatatcgcc aaaggttaca
     2101 ggctgtttga gggcggaagc gagtcggcgg agatattccc gtctgtggat ctccacggcg
     2161 cccagcgatt ccgtgaccgg ggtcaacatt tggatgtcca ccaaggtgta accccgctgt
     2221 cggaggtggg cgagcagata gagcaacccc accttggagg cattactgac ccgatagaac
     2281 atcgactcgg cggcaaataa ccccccaaga gcgactccat aaaccccacc aacaagcttt
     2341 tcgccctgcc agacctcaac gctatgggca aagcctaact cgtgaaaacg gatataggcc
     2401 cttatcattg cgggcgtgat ccatgtgttg cccctccgac cgggggcggt ggcgcaaccc
     2461 cgaatcacgc tgcgaaagtc ctgatcgagg gtgactttaa aaccgtccct cttgatggtc
     2521 ggcactaatc tcctcggaat atggatttta tcccagtcaa aaatggctct gatctcggga
     2581 gatgcccata caatggggga ttcttcatcc accggccatg ggaatatccc atgagaatag
     2641 gcgtccagca gcagatcggg gtggagggtg ccgccgacgg caacgatacc gtgctcgtcg
     2701 gcccattcag gcgacggaaa gaatcgtgaa ggcggcagga cggaagaacc aaacgcggac
     2761 ataatcccct ctgtgggtgg tatctatggg cgttgcgcga gcgacgtgcg ccgctaaatt
     2821 ggcattgccc aattctatcg actggttttt aatgggtgaa ccggttgcgc gggaatggca
     2881 caaaaagctg gccgccaatt ggtctgcgat ctactgcccc gagacaaacg acctttggta
     2941 gtgtaaaaga gtacccctat ttgatcggga acatggaggt ccccggattg caggtctttg
     3001 acaaggacgt tgaaacatcc ctggagttcg ttcgtgaccg ggcttttccg ttttctacgg
     3061 taggcacggt atcaacttcg ggcagatcac tgcgtctttt ccctaggacg tttggcgctg
     3121 ccctatccgt aaacttgccc tttaatcgca tggtggaatg gattccttcg tcaacgccca
     3181 attcaggagg aaagggccat gctgaatctg gtttcttctt gtgatcccaa ggcggggagc
     3241 ttctgggctt gtaccctggc atgccttgtc ttcggcataa gttcatattt cgtggcgtca
     3301 tctgccgccg agacgaaagt tggtaacgtc ctggaagaga cggttctttt ggacttcacg
     3361 gcgagttggt gcggaccctg ccagcgcatg gcgcccctag tcgatcaact gatcggcatg
     3421 ggttaccccg ttcgcaaggt ggatatcgac cgggagccgg ccttggccaa acgttttgga
     3481 atccaggccg tgcccacatt tgtgatgctc gtgaatggtc gggaagcgaa gcgaattgtg
     3541 ggcggtgcgg gcctggacga cttaattgcc ctatgtcggc tggcaccgcg ggcaggttcg
     3601 ccgtcggatt cgcagtcggt cgttcttacc tcggcgacaa gctccggtgt ctctccggca
     3661 tcgacgggag tgtttcccct gcgcggtgca gcgctttccg acaaggcagg ggcaaggcca
     3721 gcggccatcc cggaaaccat ccccccgctg gatgaaaact gtcgctttgc cgctgccaat
     3781 gagcaggacc tcattgcggt aaccgcgcgg attcgcgtgg aagatcccca tggccgatcg
     3841 accggctccg ggacaatcat cgacgcacgc ggcggcgagg cgttgattgt tacgtgtggg
     3901 catctcttcc gcgatagcca aggaaagaca cccatcctcg tggacctttt tggcccgaat
     3961 gccgcccaag gaattccggg tcaattgatc tactacgatg accgcttgga catcggcttc
     4021 atcagcattc gcccgaaccg ccccgtggtg gtggcgcggg tagcggcgcc cggataccca
     4081 ttagcggtgg gcgacgccgt cttctctgta ggatgcaccg ccagtcagcc gcccagcgtg
     4141 tgggaagggc gtatcacggc cttgaacaag tacctagggc cggccaatat tgaggcgtcg
     4201 cgggcaccgt ttgtcggcag aagtggcggg ggactgtttt ccaacgaagg ctttctcatc
     4261 ggtgtgtgca atgccgccga tccgccactt gatgaaggca tttatttggc cctccaagag
     4321 atccaccaat cgctcgatca gctcaatttg gcctttgtct atcgctggga cgctgcttcc
     4381 cgcggaatga ccggagaccc tcaggttgcc caaaatcgcg gacaactggc ggtgtctgga
     4441 cccggcggag ggtcctcagg gcgtggtgaa accgaaaatc gttttggcca gccacaaggt
     4501 cctagcacgg gcctatcgcc agagagcttg accctggcca ccggtttgcc gctgcccatc
     4561 ccgatgggtg atttaagcgg ctcgcgcacc gcgcaggcga tgggcacatt gggagttccc
     4621 gtgccaagtg gcggaatgcc atccgcaact gtggttcctg tgggatttgt ggagcacgct
     4681 acactttccc tggaggagca ggcgacactg gaggaaatca ggcgaagaca ggcccagggg
     4741 caccaggtgg tttgcatcgt tcaaccggcg gatcagccca actctacgag tgaaatcatc
     4801 gtcgtgcgaa gtccgtcgcc gcaacttttg gcagccctga gccaggccgg ggtgaaaatg
     4861 gttatgccgg gcgatttgcc gagccggacg gtgggcgaga ttccttcgga cccattctct
     4921 gcccggttgg gacagcaggc tagtgacagc ccgcttgtac ccattcccgc cggtgccgtc
     4981 ctgcggatcg gtacaaacgt catgcccgtt tcaggacgag cggtggcagg aactcggcca
     5041 ggcacagcgg ggtgggacta gggcacgcac cgtgatggcg attcgggcgg gatggagcga
     5101 cgattccgac gggtgcgacg acggcggttc ggtcaggcac gccgacggtt attcgggcag
     5161 tcacgtgggg cggggattgc ggtaggggcc cccccccgtg gttgccccaa aaagttcagg
     5221 cgggaacggg ggcggcgatt cgggtggggg cattctggcg ggtacggcga tcgcagatca
     5281 gggcaggcac gggggcctgc ccctacgagg ggtggttttt tcacattcgg gaacgccgta
     5341 ccgctcatcg gcgcgttata ctatcttttt tggcccgcgg tggccttgcc taaacgttga
     5401 gcctaaatgg cttgtttgcc ttgccggcgg atgagcgctg tccaacgttt atgttttagg
     5461 attcttccaa catgatcagc cttgcagaaa tcgaaaaggt tgccgtttta gcccggctgt
     5521 tattgacccc agccgaatcc caacagatga ccgagcaatt gggaaagatc ctggaataca
     5581 tgtcgctttt gaatgaggtc aacaccgaga acgtggaacc gttggcacac gcggtggaat
     5641 tgacgaatgt atttcgagaa gaccgagtca cccccagcct cgatcgcgag atggccttgg
     5701 caaatgcacc ccatcgcgat gaggagtgtt atcgcgtccc ggccgttttg ggtgaagtgt
     5761 gatactgggg catccttggg tcaagctcta tgggactttg aggaagaatg tgcgatatgt
     5821 cgctttctga attgacagcc agtcagttgc tccagcagct ccgcgagggg cgaatcaagg
     5881 ccgtcgaggt ggttgcccat tcgctgcggc agattgatca atacgatccg acggttgggg
     5941 cttttttgag agtcaaccgg gatcatgcgt tcgcccaggc ggaagagatt gatcggcggc
     6001 ggtcgcgggg ggagaagttg ggtaagttgg ccggtttgcc ggtggcggtc aaggataata
     6061 tttgcactca gggagagcca acgacttgtg cctcgcggat gctgaaacgt tttgtgccgc
     6121 catatgatgc cacggttatt gccaagctca aggcggccga tgccatcatc gtgggcaaga
     6181 cgaatctgga cgagtttgcg atgggcggct cgacggagac ctcggccttg ggccgcacgt
     6241 gtaatccttg ggatgtcacg cggatacccg gcggctcgag tggtggggct gctgcctgcg
     6301 tcgccgcccg catggtacct ctgtctgtgg gaagcgatac aggtggctcg atccggcaac
     6361 cggcagccct ctgtggcgtc accggcctaa agccgacata cggtcgcgtc agccggttcg
     6421 gactggtggc ctttgccagt agccttgatc agattggacc gcttgcccaa accgccgagg
     6481 actgcgccct gctgttggaa gtcttggccg gtcacgaccc caaggatgcc acgtctattc
     6541 cccagccagt gccgcctttt tctgagacgg tccgtcaacc gttggaggga ctgcgggtcg
     6601 gctgggttcg agagcacttg ggtgaaggcg tttcgccttc tgtcttggca gccattcagg
     6661 aggcgatcaa ggtctttcaa tcgttggggg ccaaggtgga ggaagtggaa ttgccccatg
     6721 cccgttatgc ggtggcgacg tattacatca tcgcatgtag cgaggcatcg agtaatcttg
     6781 cccgctatga cggggtccat tatggatacc gcacggacga gaaggcaatg ctggcagaat
     6841 tggccgagga gcggcgtgta ctccagcagc aggggaagag cgaggccttg gaggatttgg
     6901 ataccgcctt ggtccgcatg tatcgcaaga cgcgggccga gggcttcggg ccagaagtca
     6961 aacgccgcat catgctgggc acgtatgccc tcagcgctgg ctactacgac gcctactatc
     7021 tcaaggcgtt aaaggtccgt cggctcattc ggcaagatta cgataaggcc tttcagaagg
     7081 tccatgtaat tgtcggaccg gtcacgccga cgccggcctt tgctctgggc gaaaagatca
     7141 acgatccctt ggccatgtac ttgtttgacc tgttcacggt ggggaccaac ctggcgggtt
     7201 tgccggccat ttccatccct tgcggtttca gtgaggacgg catgccgatc gggttgcacc
     7261 tccaggcccc gccgttagag gaagaaacgc tgttgcgggc tgcccatatg ttccagcagg
     7321 tcacggattg gcatcggcga cggccgcccc tattggaccg gcaggctgtc gctacgtgat
     7381 ggttaagaca ttaggggagc caatttgctg actcacacga gtagttaggc catcttcaca
     7441 aagacaccga tctgcccatg gctgagcaac cttacacgat cgtcatcgga ttggaagtgc
     7501 acgtccaatt gctgacggag agcaaactgt tttgtggctg ttcgacgaag tttggcgcac
     7561 cgcccaacac gcagacgtgt cccatctgca tcgggatgcc gggcgtcctg cccgtcatga
     7621 atcgtcgcgc cgtggaattg gccgtgaaaa cgggcctcgc tttgaattgc gagattgccc
     7681 ggtttagcaa atgggaccgc aagaactact attatcctga ccttcccaag gggtttcaga
     7741 ttagccagta tgaccttccc atctgtcgag ggggctactt ggacgttccc agcccaaacc
     7801 gtgaggggat gcggcgaatc aggattatcc gtgtccattt ggaagaggac gcgggaaaga
     7861 gtatgcacga cgagaaggcc gggcgggctg atagccggat cgacctcaat cgcgccggga
     7921 cacctctttt ggaaatcgtc acccagcccg acatccgttc ccctgccgaa gccaaggcgt
     7981 tcttgaccga gttaaagctc cttcttaact atctggaggt ctccgactgc aacatgcagg
     8041 aggggagcct ccgcgtggat gccaacatca atctgcatat tcccacggag gatgggcagt
     8101 atgtggccac gccgattgtg gaagtcaaga acatgaacag ctttcgggct gtggaacggg
     8161 cattgaccta cgaggctcag cggcaatatg aagcatttct tgagaccggg caccgactgg
     8221 gagatgttcc caagcaaaca cggggttggg atgaggatgc tcaattgacc cggccccagc
     8281 gatccaagga ggagtccagt gattaccgct atttcccgga acctgacctg gtccccgtca
     8341 cggtgaccga tgctgaactt gaggccctgc ggcagcagtt gggcgagctt ccggccgaca
     8401 tccgccgacg attacaggaa acctatcatc tgaacgacta cgatagtgat gtcattgtga
     8461 atcaagggcg ggcatttgtc gattactttt gccagttggc ggatacctgt ggggatggca
     8521 agatggccgc caattgggcc cagcaggatg tcttgcggac gctgaacgag cgggactgga
     8581 cgatcaccca atttcccatt tccgcggcgg aactggcaag acttctttgt cgtgtcaaaa
     8641 gcggtgaact cgataccgct aaggcgcgca tggtcttcgg ccatatggtg gaccaccaag
     8701 ccaccgtgga tgaggctatc cagtcgctgg ggattgtcca ggttgacgaa acagagttgc
     8761 gccagttgtg cgcagagctc ctcgcttcca atcccaaagt cgtggcagaa gtaaagtccg
     8821 gcaaagacaa ggccattggc agtctcgtag ggcaagctaa aaaacgtaac ccgcatgtca
     8881 atcctgcaag ggtgaaagaa atctgtttgg agttgatccg gcaggaaacg acgtgaaccg
     8941 ccgtagccct ggacgaccac cacggtgctt cccctcggtt aagctgatcg gctttgtgtg
     9001 gacctccact cgcatgggca gcatgggttg catatgacgc agattgttac cctcatcacc
     9061 gatttcgggt gggaaagtgt ttacgtcgcc caaatgaagg cagtattgct ttccatcaat
     9121 cggcgtctca ccattgtgga cgtcacgcac gagatcccac cccaggacgt tcaggccggc
     9181 gccttggttc tacggcacgt tgtgcctagc tttcctcccg ggagttgcca tgtggcagtg
     9241 gtcgatcccg gtgtggggac acagcggcca tgcctttgtg ccgatatggg aggacgctat
     9301 gttgtcgctc cggataatgg gctcatcacc tatcttgccg aagtgctgcc tgttaacgtg
     9361 atggtccgcc tagaaaaccc ggcttatcgc ctgcccgagg taagtgccac atttcatggc
     9421 agggacgtaa tggccccggc cgccgcgtgg ctgagccttg gcgtgacgct ggaacagttg
     9481 ggacccgtcc atcacgcccc tgtgcttttg cccgagggat ggaagccact cgtgacggag
     9541 cggcagattc gggggaaggt cgttttggtc gatcgatttg gaaacctcat caccaatatc
     9601 gaaaagacac atctgtcgcc ggaccgaact gtgaccgtga gcattgggga gtatgttatt
     9661 ccggggttgg ttcggactta cggccatcgc acatcagggg aagtagtggc cctgattaac
     9721 tctcaaggct atgtggaggt tgcggtcgtc aacggcagcg cggccgaggt aacgggttta
     9781 ggtgtgggaa ctcctgtggt gattacttgg taagggacga tgttggacga acgagaaaaa
     9841 cagcaactcc gccagtggga tcggaccatc tattggcacg ccttcacaca gatggcggaa
     9901 tacgagcctt ttatcatcga acgcgcggaa ggttgccgtc tcattgatat cgacggtcgg
     9961 gaatacatcg atggtgtggc cagtctgtgg tgtaacgtcc atggccatcg tcatccaaaa
    10021 ctcgatgctg caatccgcga gcaacttgat cgtgtggccc acgtcacatc attgggatgt
    10081 tccaacccga cgacgatccg tctggccaaa cgtctgacgg agattgcgcc gcctggtttg
    10141 cgtcacgtgt tttttgcgga cagcggtgct ggggcggtgg aagtggccct caaggttgcc
    10201 tttcagtatt ggcggcaaat tcctcgaccg cagccccaaa agaccgtgtt tttggctctc
    10261 ggcgatgcct accacgggga cacgcttggg gcggtgagtg taggtggtgt ggatcgtttc
    10321 aatgccgtct ttcagccgct catgtttccc acaatccgcc ttccctgccc cgaaacctac
    10381 cggacacctg aaggcgttcc cccgagcgag ctaaccgagt attacctcaa ccaagtcgag
    10441 caggtcctgc ggcgggaaca tgagcggatc gtggcgatga tcattgaacc actggtccag
    10501 tgtgcggcgg gaatcgtgat gcatccgctg ggttatctgc gaggagtccg cgagctaaca
    10561 cgcaaataca atgttctctt gatcgctgat gaagtagcgg tgggttttgg tcgcactggc
    10621 cggatgtttg cctgcgagca cgaaggtgtc acgcccgact tgttatgcct tggaaaaggc
    10681 atcaccggag ggtatctgcc ggtcgctgcg acgctggtca cggacgaggt gtataacgcc
    10741 tttctcggtg actacgcctc attacgaacg tttttccacg ggcacaccta tgcgggcaat
    10801 ccccttggcg cggcggtggc cctggcaaac ttggacgtct tcgaggaaga aaggacactg
    10861 gaaaaccttc cgccgaagat tgagcgacta agggcacacc tcgagcggat cgccaatcgc
    10921 ccccacgttg gtcatgtccg ccaatgcgga ctgattgccg gaatcgagtt agtccaggac
    10981 aaagcaacta aaaaacccta cccgtggacg gaacgccgtg gctggcaggt gtgcctggcc
    11041 gtccgcgagg agggtatatt cctgcggccg cttggtgatg tgattgtgat ctttcccccc
    11101 ctggctatta gccttgagga gatcgatcag attggctttg ccgtggaacg ggccattatt
    11161 cgcgtcacag aacaggatta agaccccctc accctctggg agagggttgg ggtgagggca
    11221 accggctgtc ggagtcgatc aagaaaaccg gcattttcga gccaccctct cccgccgaac
    11281 gcgtgagggg agattatggg ataggctctc atcgagccgc cggcgccctc ggcctcggtt
    11341 ccaagcgtca aagtgtgacg ggcaaaagct cgatcccttc gctggccccc aaattgcgcg
    11401 agtacttttt ctccttgacg ttgttccagt gttcgtgaac gccgaggctg gcgagacgct
    11461 gcgtcggttc agcatgattg gggtcgtaaa atgtccccga gggcggctga tcggccaagg
    11521 ccgcctcgtg gaggtaatcg tccacgccgc ctttccgcgg gaagggcgtg ccttcggcat
    11581 agagaaagtc aaacgccacg gaatcgatgg ccaccggatc ttgcgaggca aacaagctgc
    11641 acgaccactg atcctccatc ggtgggatat gcatccgttg cggcaccgga tcgcgcgggt
    11701 gaacacccga gaaaaggccg tcgatcaggt ataggacggt cttcccgccc aaatgagcat
    11761 ggccgatgag atccaccagc ggtcgataaa tgcccgtggc ggtggaaaaa cagttgggat
    11821 ggatgtcgta gtatccttct tgcaccggcc agcgaatgag tgacccgtaa tgattcttcg
    11881 cacacagcgt cacgccagcg ccactgtggg ccttgaaatt ggcgaaattg atcaagtaat
    11941 ctgcttcggc gaagcagcga gggagaaaat cctgcgcgac accctgggga cgacaactcc
    12001 aatacaactg tgtttcagag gcctgcacct taatccgccc aaacttcccg gcgtaatcca
    12061 cgtactgcac gtcgggataa tgggcatgga ggatgtcgta atattcgtgg accaagtagg
    12121 ccagcgtgtc gcaaattgtg atgtccgccg gtttgacgcc gacctcatcg accaactgcc
    12181 gcagcagggc aagaagcatc tgcggcgagg tgttcatgta gttttcccgg cgaatgaatc
    12241 ggtaggtttc aggatcgacc acattttcgc gatagatcat gcccacccaa ttgggcttga
    12301 taaggatttt ttgccccgcc tgataaccct gggccgctcc tttgcgggtt ttgttgagat
    12361 gggagaacag ccgatgccat gcgtctttga cggagtctgc tcccgtcaag tggcgaacgg
    12421 cttggtccat catcgcgttg acccgcgatt gcttgacgtg atcgccttcc caccatcggc
    12481 catcgtcagg gcctttccaa tcaacaacac cgggatcgtg gacccagaca acccgcccgg
    12541 ggaagatacc ctttccctgc cctatgggct tgagagggac cggctgatcg gcgctgacct
    12601 cggccgccca ggcacccaaa cgtgctcccg cgattcctgc cagggcagtc gagcctccaa
    12661 taaaatgccg acggttaatc tttagacttt ctggcaattc ggcggcaaat gggacgtcat
    12721 catggcgcga catggcaaag cctccggaaa aagggacatt cattccaaac gctgcaatgt
    12781 tatgctggag ctatcctggg gcagaaatat ttaacgtctt tgagggatga tcgctccgct
    12841 acccccagct atgcggcttt tggcaacacc cataaatata accgtggcgg gctcgaaatg
    12901 caccaaacac ccgcccgact ccgcgttcca gcggccgggc tgaaggtgac atcgtccacg
    12961 tctcgaaccg ccataaggaa cgccgccccg ccgatcaggc gaaacaccga tgaccggggc
    13021 aagcaggctg gggcttcatt cgccacaact agtgggcccg ttgtccacag aatagtgatg
    13081 cgctaatgat tcgcatcttt agtcgactgg attttccaca gctcgtactg cgaaccaccg
    13141 ctccgggatc cgaaaccgaa cgcataagcc cagaggagtc gagagtcgtc ccatgggacg
    13201 agaatcaaat aaaaaacgcc ggaaaaatcc ggcgtaggga acgagaagcg agggcgacgg
    13261 tgcgaacaaa ttggcgacag gaaagggtaa cctccagatc gtgggagata gtcttcagca
    13321 tagacgtcgg gcagctgtta ccgaccagga tagaccccat cgcagtgggg agatcgacaa
    13381 cactagagta tcattgcgtt ggtcttggcg acggtttaga attcatcgcc gccagtgata
    13441 tctgtgatct gcccggaaga ggggcttttc gcgctgatcg gacggcggag ggcttaggct
    13501 ttcggtatga gacttaggaa gatcgacatc cgtctggaag gccccgatat ccaaccggag
    13561 agtattcccg ttggagagct gctcgacttg cttctgtgtt acgaacgact tttacgtgat
    13621 ctgacgggga aacggcactc taaggtcgag agaaaacatc gagagatgcg gcttcggctg
    13681 gtatcagtgg aaaggggaag taccacctta agttgtctcg ctccggagga actttacaac
    13741 gcgaaccgca caatttccca ggctataggc aaggggtccg atttgccacc agaagccagg
    13801 gactcttttt ttaggctccg aaaaaggatt gagaagctgg gttgtgatgt caagattcaa
    13861 ggtgaagact tggagccatt tgcgatttcg cgtgagcggg tgcccacaaa gaggtttatt
    13921 cggggcgaga caactgttta cggagaatgc attgcggtcg gaggacgtaa ggccgttgtt
    13981 aaattggtga gcggtagcgg ggatcaaatt agaatccgtc ttagcaacga attagccaga
    14041 gagattgcga agcatcttta cgaggaaatt ggtgttcggg gactggcgac atgggaagtg
    14101 agcggaccgc ggatcgtaga tatggaggca tatgagcttt tgccctatcg tcggacatcg
    14161 ccgaaagagg cgattcaggc attgcaagag agcgccggta aggactgggg caagatcgcc
    14221 gatcccgagg aattcttgag gaaactcagg agggagtaaa ggggcgccct acatgggaaa
    14281 gcgacgaagc gttatcctcg ataccaacat tcttgtatat gcaatccgtc ccgatgattg
    14341 taacgatgat agggcaaaaa gggcgaagga ctttatcagc gatatcagtc gggatgatga
    14401 agctgatatc ttgatccctg cggtggtggt cgccgaattt ctctgcggtt gcgactctgg
    14461 gttcgaagac gcagtgcgga accttcagga gcactttaag attgtcccct tcgatatgaa
    14521 cgctgcctgt cagtaccgtc aagtggtgac tacgaggagg agacgatcgg gtagtgatca
    14581 ggatgagacc aagcaagtgg ccgcggcgga gagaaatact gttgacgaaa tgatcgtggc
    14641 cacggcacta gctgccagag cccaggaatt gatcacatac gaccagggac ttatcaattt
    14701 ggcaaaggcc gttggcattg atgccaagga accgccggag ttgggtctag gtttgcttcc
    14761 ggggttttag gcgccaaacg aacaaccttt ccggtcttac tgcatccgtg cgttcgagta
    14821 attggcagtt tgcgtagctg gcttgcgttt tttttccacc gtgaagcaag actgggccct
    14881 aagatcacgc accggtttca gaaaaaagcg gtaatggttg ctaaagcaga atttgagcga
    14941 tccttcatgc accgcctcgt gggaatttct tgaaaagtcg aaggaaagaa agtcttagct
    15001 gatctttttc tgaacgtttt gaaggcgtcg gagaattatt tcagcaggcc agcgtggata
    15061 gttaagcgct tggcaaacgg catcggaaga gagcattcgc cgtcatcggg ataatgcaat
    15121 agctcgtcag tggtatactt taagccgctt gggcatgtct ttgtctctgc cagtttaacc
    15181 aaagtatttt ctaaagcggt acttgtgaca aatcaccgct acggcgtttg ccgtgtgcca
    15241 caaagttgtg ctccatgatg cgaaccttct tttggggaac acgagtacag caagggtgat
    15301 gccgttttgg aagtgttgga ggagaacaaa aaaacgccgg aaaatccggc gtatggaacg
    15361 acaagcgagg gcgacgggac tcgaacccgc aaccac
//
LOCUS       DAOSUT010000093        15208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2385_length_15208_cov_9.545371, whole genome shotgun sequence.
ACCESSION   DAOSUT010000093 DAOSUT010000000
VERSION     DAOSUT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 15208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15208
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_15208_cov_9.545371"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLD05_09675"
     CDS             <1..207
                     /locus_tag="PLD05_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77751.1"
                     /translation="GEAAILWAPHWDRISQLVKEGKLPGKQVSENVLILDELDPRHYE
                     VLTSESSVVADWENPVMLIRAKKD"
     gene            complement(250..1836)
                     /locus_tag="PLD05_09680"
     CDS             complement(250..1836)
                     /locus_tag="PLD05_09680"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOP77752.1"
                     /translation="MAPHPAIIRVACPTVYTLSVLLWLGVGGNITSGQSIFDALPHLG
                     QGKTVAENGLWIETPLDRQFRQSKEVTIAEVQGPGVITMIHFALPQRMVAQPDKYRLG
                     RELAIRAFWDNETTPSVECPLVDFFCDPMGERESLDTLMVNKKRGWNAYFPMPFRKSA
                     KLTLSYDGPVEPGQELWELMPCYSYVMVRKVEELPANVGYFHAQWRQQLINLGKEDYV
                     SLDARGKGKFVGWNVTIRLPGQPGYPVDENEKFYVDGEETPSIEFQGLEDSFGFSWGF
                     PPEESTFLRTGWFPFHKDGAAAYRFFLEDSISFDRSLKVTIGFGANEHPTFRSRFGKP
                     GNELELSTTVYWYQSEPHAPFPALSPAEQRRPTERSWKDMENLPTSEALQSRGVKLHF
                     RCGRPENETIFAVPWYAAEVKQGFAYTGWPFPVFHTRAHEKEVQISLTVPTGRTGILR
                     LYMIDPDHFMGGRHQEVFIGDTSFGEIRDFDDGRWLEIPVDASMTSAGSLLVRIVNRN
                     PRSNAVVSILEWVEAAASNR"
     gene            2184..3728
                     /locus_tag="PLD05_09685"
     CDS             2184..3728
                     /locus_tag="PLD05_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOP77753.1"
                     /translation="MTQSNAPAEEKGLPMTAKWDPEKLAREEAFLQELEKKSLWERIR
                     GYAKLTGPAWLQSAMTLGAGSAAASVMSGASFGYKLLWVQPLAMFCGVIVFAALAKIV
                     LTTGERPYRSFGRELHPIVPIFWALGTIVASIIWHFPQYTLAGAAAWDLAQVVQTNVF
                     DQKQAEGPGQQTEPGDKPQQGQNTTQMRGLELLVKYGAAACILMINIFTVWNYGSHAK
                     GIKLYEGFLRWVIRIVILAFLAVVIFTGINLKELFWGLFGFYIPETTEGITVVLGALG
                     AAVGINMTFLYPYSILAKGWGKHHRGLAKWDLFSSMLVPYTIVTSLIIIAMANTVYDG
                     KDAVRQGLQPVEAAAAFSTLMHSDLGRMILDLGFLAMTCGAISTHMVVCGFTVCEMLG
                     LQYTRARFRLFSLTPVIGILGAGMKLPFWLPILASAICLTMLPIVYLSIMIMANNRKY
                     IGDAVGSGFTRWVFNFLLLVAITVTTIGTVVQIKLKVVDPVRKWLNPPAAVQSATPSQ
                     AGEEKK"
     gene            complement(3822..5024)
                     /locus_tag="PLD05_09690"
     CDS             complement(3822..5024)
                     /locus_tag="PLD05_09690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOP77754.1"
                     /translation="MTVHVSRLLQTLEPSATLAMAAKAKELKAKGRKIYDLSLGEPDF
                     ATPEHICQAAIEAMRAGHTHYTVASGIPELKQAVVDQYKKRYGLTYSPQQVVISNGAK
                     HALHNAFTATLNPGDEVIIPAPYWVSYSELIKLTGAVPVIVKTEESSGFKLNAAQLRQ
                     ALTPRTRMLLLCTPSNPTGTFYTKSELEGIADVVLDADLTVVADEIYDQLIYTAEPFT
                     SFPTVRPGLQERTILINGVSKTYAMTGWRIGWAIAPTLEVAKAMGDLQSQETSNPCSI
                     SQYAALAALTGPQDCVETMRREFERRRDYVAQRIAKLPGVTAAPMQGAFYAFINISQH
                     LGKRYGGTLVENSSEWCLELLAQKGVATVMGSAFGAEGYARLSFATSMETLAAAFDQI
                     EAFLQEAK"
     gene            5329..6549
                     /locus_tag="PLD05_09695"
     CDS             5329..6549
                     /locus_tag="PLD05_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOP77755.1"
                     /translation="MMAQSGKVGLWLIGAKGGIATCVSVGWAMLTRSLTSKVGLVTEL
                     PQFAQAGLVDWPDIVIGGHEIRDVSLAEESRAFASVNRTFERQHVDLIADALESIEQQ
                     IRYGTVFGADQMIAQLASPNVPRDRSAREAIERIAADLKDFKSRHNLRSLIVINVAST
                     EPPVDPSQFPAKWSELEQLLAEQPDPGLPTSTLYAIAAFETGCPYINFTPSLGPALPA
                     LDELAQHNNVPYMGCDGKTGETLMKSVLAPMFRARNLRVLSWVGHNIFGNLDGKILDD
                     PRHKSSKVQSKDRVVSGVLGYHPQTLVSIEYIEGLGDWKTAWDHVHFQGFLGVPMVLQ
                     FIWQGCDSILAAPLVLDLCRLTERAHRIGIVGLMPFLASFFKSPYDVKEADFFKQFQM
                     LEQWIESVSHSHPS"
     gene            complement(6575..7372)
                     /gene="lptB"
                     /locus_tag="PLD05_09700"
     CDS             complement(6575..7372)
                     /gene="lptB"
                     /locus_tag="PLD05_09700"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOP77756.1"
                     /translation="MRLFTGRAVQPIAQNGPHIRAVPGAILAACGLVKNFGKRRVVDG
                     VSFEVWPGEIVGLLGPNGAGKTTSFRMTCGLIRPDAGQVFLQGQDVTHWPMHRRAREG
                     GMGYLAQESSVFRKLTVEENLLGVMELLGFDRKLRLRRCRELLEQFDIVQLAKAKAGG
                     LSGGEKRRLEIARALVSNPRIILLDEPFTGIDPVTVDAIQEIIRNLRAQGISILITDH
                     QVRETLQVTDRSYVIRAGRVLCHGTPEEVIANPEARKSYFGEDMQLN"
     gene            complement(7624..9492)
                     /gene="lepB"
                     /locus_tag="PLD05_09705"
     CDS             complement(7624..9492)
                     /gene="lepB"
                     /locus_tag="PLD05_09705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOP77757.1"
                     /translation="MKQTVHWLRGMTQFFRGKGSGHLAGKERVSSRQSQEGQQSRARM
                     GKNRPDSQEGAPGREENTRASNWLREVVEAISIALILAFLFRAFEAEAFVIPTGSMAP
                     TLMGRHKDLICERCGFSFQTGASEEVDKLTNRPTGRFVLSATCPNCRYTMYVGPNKDD
                     GPPTPSYKGDRILVSKYEYHLSEPRRWDVIVFKYPGDASTNFIKRLVGLPNETVRIYR
                     GDVYIRPAGSDQFAIARKPPEKILALLQPVYMDDFQSPELKAAGWPDRWQPEVGQQGL
                     WEIGENPRQYRVTAPEDMEVWLRYQHIVPSWEHWQAILEGNPIDAKQVSPQLISDFMA
                     YNTNQSDESDYGPHTLRTVRIPPRGDQLGLHWVSDLAVECEADMESDGGEIILELGAG
                     KNRYQARLNLADGVARFIGMGLRSFSEEAPIHIHGRGHYQLRFANVDHQLVLWVNGRP
                     ITVEYEAVWSGPTLDDRDRPARIGVRGGTVTIRHVRLFRDVYYIAHDRRSGLLMHDFD
                     WERSPYRSLIPSPFAMDQFRQMVAEVMSDPTQWEFFQYANHLEFALGPDQFFVLGDNS
                     PQSEDGRFWTKKSYVQRELLVGKALAVIWPHSLDRIPGTKIPIRFFPNFWRMKVVR"
     gene            complement(9747..>10751)
                     /locus_tag="PLD05_09710"
     CDS             complement(9747..>10751)
                     /locus_tag="PLD05_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77758.1"
                     /translation="FTILKSGGVEQFVAACDRIRQERTPKPRIQKPAEDRLTLKSVGQ
                     CLEEVKNSGIISIGGGNKGVAEAIQQQLGIPVHFVSFEEMAEAAEAADKAQVDELAEK
                     WRKEAQKVLIDEPEETLRLSAANYLAQKALLNKYRAEAITINCLGGFYSGKLKAYPCL
                     GFVELLNSGFVGACEADLPSTVTMVMVNHLAGRPGYISDPVIDTSKREIIYAHCVATT
                     KPFGPEGPANPYEILTHSEDRRGASVRSFLPVGYMTSTMEIFPDRKQILFHQGVAAEN
                     VVIDRACRTKLGCEVWGDLDKLLLGWSNGWHRVTFYGDLKDAVKELAAALQFNLVEEA
                     "
     assembly_gap    10752..10851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10852..11114)
                     /locus_tag="PLD05_09715"
     CDS             complement(<10852..11114)
                     /locus_tag="PLD05_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOP77759.1"
                     /translation="MELHSRDALMCRCCANRRRFLKSCAGCGVAALAASSLRLAPAES
                     QESSAIPASSDRVRVRLVFACWALVQDRPTWPHIGHDMRPEIER"
     gene            complement(11419..12006)
                     /locus_tag="PLD05_09720"
     CDS             complement(11419..12006)
                     /locus_tag="PLD05_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOP77760.1"
                     /translation="MEPEHLPPRSPELMSADNTALLVIDVQERLIPHITDHKRIVWNI
                     RRLIDAATILGVRVVGTEQYPKGLGPTVPVLAERLGTLPEKLMFSCRECWNLFNELKA
                     QGMRNLLVTGIETHVCVQQTVLDFLAQGWGVYVAADAVGSRHRIDYEIALRRMEVSGA
                     VLTTTEAALFEWCVRAGTDQFKKISRLVQESLPDE"
     gene            12227..13051
                     /locus_tag="PLD05_09725"
     CDS             12227..13051
                     /locus_tag="PLD05_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOP77761.1"
                     /translation="MRSIAIMNQKGGVGKTTTAVNLAAAIAQEGYKTCLIDMDPQAHA
                     SLHLGIEPQIEMLSIYDVLVRDRPLAEVRRQINDHLWVVPSHLDLAAAEVELAGVVGR
                     EVILRDKLREDPEKFDFLFVDCPPSLGILTINALAAVNEVFIPLQPHFLALHGLSKLL
                     QTIELVAKRLNNRLRLTGIILCMFDSNTRLAAEVSEDVGAFFGNHRERKSPWAGAHMF
                     QTRIRRNIRLAEAPSFGQSIFQYSPDSNGAEDYRNLAREVIQQMPVAENSSFKKAC"
     gene            13738..15012
                     /locus_tag="PLD05_09730"
     CDS             13738..15012
                     /locus_tag="PLD05_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HOP77762.1"
                     /translation="MHTGWLAPLNGGHSGLTAAPEPRDAGREVGVVGRPDGRGMGVVP
                     PETAPGHPLSSLSHQASPGCYRDLLEIPTSGTSVADCPGCFDSCGDPQSCWQWFIEPA
                     GLIMGRNDPNRVWFSYQSNNNANELMHSQQARTSWQGGWELTIRRTFACDNWRLEGTY
                     WGIAQMRGDASATHPEFVSSTLIFTDVVYADPTIPGMPEDLFFGTYEQKLWRTNEIHN
                     VEVNLVRHRILWDIHRLGIDWLVGVRYFRFDERLTYGSRAHDGFWGEYPELEGYLSDR
                     IENNLIGIQLGFDLQYPIGKSLQLACTPKIGLYNNHIHNRFDAYRGDGALFAPNPNPP
                     VGDPVPGRYPVISSDDRFATLAQLDARVQWHVTPRWLMFIGYRLVSVSEIGLADAQFP
                     CNVVDIVDAISHIDSNGDLLLHGAFAGMSIAF"
     gene            complement(15019..>15208)
                     /locus_tag="PLD05_09735"
     CDS             complement(15019..>15208)
                     /locus_tag="PLD05_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77763.1"
                     /translation="KGDFLRLPPGTPHQFITEEDVLEMLNIHCPGSRPNRDVYFLGET
                     PEGFSSAGEIHNEPPSGL"
BASE COUNT         3391 a         4048 c         4101 g         3568 t
ORIGIN      
        1 ggagaggcgg ccattttatg ggctcctcat tgggatcgaa tttctcaact cgtcaaggaa
       61 ggcaagctgc cgggaaaaca ggtcagtgag aatgtgctta tcctagatga gcttgatcca
      121 aggcactatg aagtactcac ttcagaaagc agcgttgtgg cggactggga aaatccggtc
      181 atgcttatta gggcgaaaaa agattaacga ttggcaggga cactaattgc gattccagaa
      241 accacggaat caacggtttg atgccgcggc ttcgacccat tcaagaatgg ataccacggc
      301 attcgagcga ggatttcggt tgacgatgcg aaccaagaga gagcctgccg aggtcatgga
      361 cgcgtcaacc ggaatctcga gccaacgccc gtcatcaaaa tccctgattt cgccaaacga
      421 ggtgtcgcca ataaagacct cttggtgacg accgcccatg aagtggtcgg gatcaatcat
      481 gtaaagccgc aggatgccgg ttcttcctgt gggcacagta agtgagattt ggacctcctt
      541 ttcatgggca cgtgtatgaa acacggggaa aggccaaccc gtgtaagcga agccttgttt
      601 gacctccgcc gcataccatg gcactgcaaa aatcgtttca ttttcgggac gtccacagcg
      661 aaagtgcaac ttaacgccgc gactctggag tgcctcgctc gtcggcagat tctccatgtc
      721 tttccacgat cgttctgttg ggcgacgttg ttccgccggg gatagggcag ggaagggggc
      781 atggggttct gactgatacc agtaaacagt cgttgataac tctagttcgt taccgggttt
      841 tccaaatcga gatcgaaacg ttgggtgctc gtttgcgccg aaaccgattg ttaccttaag
      901 cgagcggtcg aaagaaatgg agtcctccaa aaagaagcgg taagctgccg ccccgtcctt
      961 gtgaaaagga aaccaccctg tccgcaagaa ggtactttcc tctggcggaa atccccaact
     1021 gaagccaaag gaatcttcca atccttgaaa ttcaattgag ggtgtttcct caccatctac
     1081 gtagaacttt tcattttcgt ccaccgggta accgggttgg ccgggaaggc gaatcgtgac
     1141 attccatccc acaaatttgc ctttcccgcg agcatcgagg gacacgtaat cttctttgcc
     1201 gagattgatg agttgctgtc gccattgcgc atgaaaataa ccgacattcg caggcaattc
     1261 ttcgacctta cggaccatca catagctata acaaggcatc agttcccaaa gctcctgtcc
     1321 aggctctaca gggccgtcgt aagaaagtgt caatttcgcc gacttgcgaa acggcatggg
     1381 aaagtaggca ttccaaccgc gttttttgtt gaccattaac gtatcgagac tttccctctc
     1441 tcccatggga tcgcagaaga aatcaacgag tgggcactca acactcggcg ttgtctcatt
     1501 atcccaaaac gcgcggatgg ccaactcacg tcctagccga tatttgtccg gctgcgccac
     1561 catgcgttgg ggaagggcga agtggatcat ggtgattacg ccgggcccct ggacctcggc
     1621 aattgtgacc tccttcgatt ggcggaattg ccgatctaaa ggcgtttcga tccaaaggcc
     1681 attttctgct acagtttttc cttgccctag atgcggtaaa gcgtcaaaga tggattgacc
     1741 gctcgttatg ttgccaccga cgcctaacca cagaagcacg ctcagcgtgt acaccgtagg
     1801 acaggcaacc ctgataatag cggggtgcgg tgccatgtct cttcccttcc gtttctggcg
     1861 gacaagattc aggtgtgaaa acaacattcc gaaacgcaac tcgccacagt acgctgtcgc
     1921 tcggctattg cctggacgtg caaccagcct aatacacatc aatccatttc acaagaagta
     1981 catggcgaaa ttccccgaag taaccttgaa atgtgaatct gtcgagacta accttggtct
     2041 atccggtttt gcgatggcga cgagagactg tcattggtac cgtgaggtgg tggcgaacac
     2101 ctctaactcg cagttcgttc ttgaagggaa gttttctggc ggctcggcgt cctgaaaaac
     2161 atacttgcgc gagggagaca gttatgactc agtcaaatgc acctgcggaa gaaaaaggcc
     2221 ttcccatgac ggccaaatgg gatcccgaga agttggctcg ggaagaggca tttcttcaag
     2281 agttggaaaa gaaaagcctt tgggagcgga tccgtggata tgcgaagctc acgggtcctg
     2341 cctggctgca aagtgccatg accttgggag ccggtagtgc agccgcctct gtcatgtcgg
     2401 gtgcatcttt cggttacaag ctcctctggg tccaaccgct cgccatgttt tgcggtgtca
     2461 tcgttttcgc agcgctggca aagatcgtcc taacgacggg cgaaagaccc tatcgatcat
     2521 ttggccggga attgcatccc atcgtgccga tcttttgggc tcttggaacc atcgttgcat
     2581 ccatcatttg gcactttccg cagtacacac tcgcaggtgc cgccgcgtgg gacctcgcgc
     2641 aagtcgtgca aacgaacgtg tttgatcaaa agcaggccga gggcccgggg caacaaaccg
     2701 agccaggcga caagccgcag cagggtcaga atacaaccca aatgcgaggc cttgaattgc
     2761 tcgtcaagta tggggccgcc gcatgcatcc tgatgatcaa cattttcacc gtttggaact
     2821 atggaagtca tgccaagggg atcaagctct acgagggatt cctgcggtgg gtgatacgaa
     2881 ttgtgattct cgcttttctg gctgtggtga tttttaccgg cattaacctc aaggaactat
     2941 tctgggggct gtttgggttt tacatccccg aaacgacgga agggatcacc gttgtcctag
     3001 gtgcgttagg ggccgccgtg ggaatcaata tgacgtttct ctatccgtat tccatcctgg
     3061 ccaaaggttg ggggaagcac caccgcggtt tggccaaatg ggatcttttt agctcaatgc
     3121 ttgtccctta cacaattgtg acgtccctca tcatcatcgc gatggccaac acggtttacg
     3181 acggtaagga cgcggttcgc caggggcttc aacccgtcga agccgctgca gccttcagta
     3241 cgcttatgca ttcggatctg ggacgtatga ttctggatct cggtttcttg gccatgacat
     3301 gcggtgccat cagtacccat atggttgtct gtgggttcac cgtttgtgaa atgctgggct
     3361 tgcagtatac gcgtgcccgg tttcggcttt tctcgttgac accggtaatc ggcattcttg
     3421 gggccgggat gaaacttccg ttttggttgc cgattcttgc atcggccatc tgcttaacga
     3481 tgctgcccat cgtttacttg tctatcatga tcatggccaa caacaggaag tacatcggcg
     3541 atgcggtggg gtcgggattc acccggtggg tcttcaactt tttgcttctt gtggcgatta
     3601 ccgtcacgac gattggaaca gttgtccaga tcaagctgaa agtggttgat cctgtccgga
     3661 agtggctgaa cccaccggcg gctgtgcaat cggccacccc aagtcaggcc ggcgaggaaa
     3721 agaaatagac aacccttacc ttgcgctgat tcattgagaa atgaggcaac ccccgactcc
     3781 agacgaaatc cctgggtcgg gggttggttt gcgcctaggc gtcatttcgc ttcctgcagg
     3841 aaggcttcga tctggtcaaa tgcggctgcg agagtctcca ttgaggtggc aaaggagagt
     3901 cgcgcatagc cttccgcacc aaaggcggag cccataacag tggcaacgcc cttttgggcg
     3961 agtaactcga gacaccattc gcttgaattc tcgactaacg tccccccata gcgcttgccc
     4021 aggtgttgcg aaatgttgat aaaggcatag aaagccccct gcatgggcgc ggccgtgacg
     4081 cccggcaact ttgcgattcg ttgcgcgaca taatcacgac gccgttcaaa ctcacgccgc
     4141 atcgtttcga cacaatcctg ggggccagtc agggcggcca gggcggcata ctggcttatg
     4201 ctgcaagggt tggaggtctc ttggctctgt aggtcgccca tggccttggc cacttcgaga
     4261 gtaggggcaa tggcccaacc aattcgccac cccgtcatag cgtaggtctt gctcacccca
     4321 ttaatgagaa tagtgcgctc ttgcagtccc ggacggaccg tgggaaagct ggtaaacggt
     4381 tcagcagtgt aaataagttg gtcgtaaatt tcgtcggcga cgacggttag atcagcatcc
     4441 aagacgacgt cggcaatgcc ttcgagctcg ctctttgtgt aaaacgttcc tgtgggattg
     4501 gaaggcgtgc acagaagaag cattcgtgtt cgtggtgtca aggcctgccg taattgagcg
     4561 gcatttaact tgaatccgga agattcctcg gttttcacaa taacgggcac ggccccggtg
     4621 agcttgatca attccgaata gctcacccaa tagggtgccg gaatgatgac ttcgtcaccc
     4681 ggattgaggg ttgccgtaaa ggcattgtgg agcgcatgct tcgccccgtt tgagataaca
     4741 acttgttgag gcgaataggt tagcccatac cgctttttgt actgatcaac cacggcttgt
     4801 ttgagctcgg gaataccgct cgccaccgtg tagtgggtgt ggcctgcccg cattgcctcg
     4861 atcgcggcct gacaaatgtg ttccggagtt gcgaagtccg gctctcctaa actcaggtcg
     4921 tagatcttcc gccccttcgc tttaagctcc ttggccttgg ccgccatggc aagagttgca
     4981 gaaggctcca gggtctgaag cagacgagaa acgtgaacgg tcatcggatc cctctcgtga
     5041 cttgtttact gatcaaccaa ctgtgcctat ttcacgcatg ttgactgcca acggtagttt
     5101 atctacaatt agtcttaaga taatcagcag tctgatttga tccaagtcga ggggggcagt
     5161 ttttcctatc aagggttgtg gaagagccgc ttgcctcgcc catggggtgg tcttgtgcga
     5221 ttggctcgta gccgccgtga tgatcgtcca gtggggatgg tgggggggaa ctgttgccga
     5281 tgaattgcgc ctagtcggtg aggttgatat tggaacaagg atcacacaat gatggcccag
     5341 agtggcaagg taggtctttg gcttattggc gccaagggtg gcatcgcgac ctgcgtcagt
     5401 gttggctggg caatgctgac gcgaagcttg acatccaagg tcggtctcgt cacggaactt
     5461 ccacagttcg cccaggcggg acttgttgat tggccggaca tcgtcatcgg aggccacgaa
     5521 atccgcgatg tatctttggc agaggaatcc cgggcatttg cgtcggtcaa tcgcacgttt
     5581 gagcgacaac acgtggattt gattgccgac gcattggaga gtattgaaca gcagattcgt
     5641 tatggaacgg tctttggcgc cgatcagatg attgcccaac tcgcttcgcc caatgtgcca
     5701 cgtgatcgct ctgccaggga ggccattgag cggattgccg cggatttgaa ggacttcaaa
     5761 agtaggcaca acctccgatc gcttatcgtg atcaatgtcg cgtcaacgga accaccggtt
     5821 gatccgtcgc agtttccggc aaaatggtcg gaactggaac aattgctggc cgagcaaccc
     5881 gaccccggtc tgccgacaag taccctatat gcgattgctg cgttcgaaac ggggtgtcct
     5941 tatatcaact tcacgccgtc tctaggacca gctttgccgg cactggacga gcttgcccaa
     6001 cacaacaatg ttccctacat gggctgcgac ggcaagacgg gcgaaacgtt gatgaaaagt
     6061 gttcttgcgc cgatgtttcg ggcaagaaat ttaagggttt tgagttgggt tgggcacaat
     6121 atctttggga atctcgacgg caaaattctg gatgatccgc gacacaagtc aagtaaggtc
     6181 cagagcaagg accgcgttgt ttccggtgta ctggggtacc acccgcaaac gttagtcagc
     6241 atcgaataca ttgagggcct tggtgattgg aagacggcat gggaccacgt gcactttcag
     6301 ggatttctcg gagtgcccat ggtcttgcag ttcatttggc aaggttgtga ctcaattctg
     6361 gctgccccac tcgtgcttga tctttgtcgt ctcacggaaa gggcacaccg gatcggcatt
     6421 gtggggctaa tgcctttcct cgccagcttt ttcaaaagcc cctacgacgt caaagaagcc
     6481 gatttcttca agcaatttca aatgcttgaa cagtggattg aatctgtcag ccattcgcac
     6541 ccctcatgat ttcgccagtt ggggctggac agagttagtt taattgcatg tcttctccga
     6601 agtaggattt cctggcctca ggattggcga tcacttcttc gggagtcccg tgacacaaga
     6661 cccttccggc ccgaatgacg tagcttcgat cggtcacctg gagcgtttct cgcacttggt
     6721 gatccgtaat cagaatagat attccttgag cgcggagatt gcgaatgatc tcctgaatgg
     6781 catccaccgt cactggatcg atcccggtaa agggttcatc gaggaggatg atacggggat
     6841 tagagacgag tgcccgggca atctcaagtc gtcgcttttc accgcccgac aacccgccag
     6901 ctttggcctt tgccaattgc acaatatcaa attgttctaa aagttcacga cagcgtctta
     6961 accgcagttt gcgatcaaac cccagcaatt ccatcacccc aagaaggttt tcttcgaccg
     7021 ttagcttacg gaagacgctt gactcctgag caagataacc cataccgcct tcgcgcgccc
     7081 gccgatgcat gggccagtga gtcacgtctt gtccttggag aaagacctgg ccggcgtcgg
     7141 gccgaatcag cccgcacgtc atgcggaaac ttgtggtttt tccggcacca ttcggtccaa
     7201 gaagaccgac gatctcgcct ggccacacct caaaacttac gccatcgacc acgcgccgtt
     7261 ttccgaagtt cttgaccaat ccgcaggctg ccaagatggc accgggaacc gcccggatat
     7321 ggggaccatt ttgagcgatg ggttggaccg cacgtcctgt aaacagcctc atatgtcgct
     7381 ccgtctgatt agaaagctct ccgtccttcg tccacaccaa ataagaacct atcccataat
     7441 cccccctcgc ccatttggcg ggagaggggg tcgggggtga gggtggatgg aatcgctgcc
     7501 ggttttctca atcgattccg acgttcgctt cccccgcccc aaccctctcc cagagggaga
     7561 ggaggattct ttgggatagt ctctaacctg ctacggtaac tgcttcccct gataatcaag
     7621 tgattatcgt acgaccttca tcctccaaaa attcggaaag aagcgaatgg gaatctttgt
     7681 tcctggaatc cggtccaggg aatgtggcca gatcacggcc aaagctttcc caacaaggag
     7741 ttccctctgc acgtaggact ttttcgtcca gaatcggcca tcttcgcttt gcgggctatt
     7801 atcaccgagg acaaagaatt gatctggtcc aagagcgaac tccagatgat tggcgtactg
     7861 gaagaattcc cattgcgttg gatccgacat gacctcagct accatctggc gaaactggtc
     7921 catggcgaaa ggactgggga tcaacgagcg atacggcgac cgctcccagt caaaatcgtg
     7981 cataaggagg ccggaccggc gatcatgtgc gatgtagtac acatcacgaa agagacggac
     8041 gtggcggatc gtgacggtac cacctcggac gcctatacga gccgggcggt cacggtcatc
     8101 cagtgtcggc cctgaccaga ctgcctcata ttccacagtg ataggccgac cattgaccca
     8161 aagtaccaat tggtgatcga cattggcaaa cctgagttgg taatgtccgc gtccgtggat
     8221 gtgaataggt gcctcttcag aaaagcttcg gagccccatg ccaatgaacc tcgcaacgcc
     8281 atctgccagg ttcaaccgtg cctgatagcg attttttccg gctcctagct ccaagataat
     8341 ctctccgcca tcgctttcca tatcggcttc acactcaact gctagatcgc tgacccagtg
     8401 cagcccaagt tgatcaccac gcggcggaat acgaacggtt cgaagagtat gagggccgta
     8461 gtcgctctcg tcactttggt ttgtattata ggccataaag tcgctaatca attgcggaga
     8521 gacctgtttg gcatcaatgg gatttccctc aaggatcgct tgccagtgtt cccacgaagg
     8581 gacaatatgc tggtagcgaa gccacacttc catgtcttca ggggccgtca ctcgatattg
     8641 ccgcggattt tccccaattt cccaaagacc ttgttgaccc acctccggtt gccaccgatc
     8701 aggccatccg gctgccttga gctcaggtga ttgaaagtca tccatataga ccggctgcaa
     8761 tagggccaga atcttttccg gcggttttct ggcgatcgca aactggtcac tgcccgccgg
     8821 ccggatatag acatcccccc ggtaaatgcg gacggtctca ttgggcaagc caacaagccg
     8881 tttgatgaaa tttgttgagg catctcccgg gtatttgaag acgattacat cccagcggcg
     8941 tggctctgaa aggtggtact cgtattttga cacaagaatg cgatcgccct tgtacgacgg
     9001 agtcggtggg ccatcgtcct tgttcggacc gacgtacatc gtgtagcgac aattagggca
     9061 cgtggcactc aagacgaaac gtcccgtggg tcgattcgtg agcttgtcaa cctcttcgct
     9121 agcacccgtt tgaaaactga agccgcagcg ttcgcagatc aagtccttat ggcgccccat
     9181 cagtgttggt gccatggacc ccgttggaat cacgaatgcc tccgcctcga atgcccggaa
     9241 gaggaacgcc aaaattagag cgatcgagat cgcctcaacc acctcgcgaa gccagttgga
     9301 ggcccgtgtg ttttcctcgc gcccgggagc tccctcttgg gaatccggcc ggtttttgcc
     9361 cattcttgcc cgtgactgtt gtccttcttg agattgacga gaactaaccc gctctttccc
     9421 agccaaatgg cccgaacctt tacctctaaa gaactgcgtc atcccgcgca gccaatgcac
     9481 cgtttgcttc atttcgctta cactttcaaa atcgatgtat caattacggc caaaaaacga
     9541 acgtacctgg caaaatgctc caggaaggcc atgggatcgc cacggcagaa cctaaatgtt
     9601 aatgtaacaa atctctgcca acctaagcag atcacatttg gtggcagctt tcttctgctt
     9661 ggggccccgc gacacctttt agatgtcaag ctactgaacc gaagatgtgg gccactttga
     9721 gtgcccgttt agtggggtaa cgcgtatcaa gcctcctcca ccaggttaaa ctggagggca
     9781 gccgccaact ccttgacggc gtccttcaga tcaccataga aggtgacgcg atgccacccg
     9841 ttgctccacc ccaataacag cttgtccaaa tccccccaaa cttcgcaccc caatttggtg
     9901 cggcatgctc ggtcaatgac cacattttcc gccgcgacac cctggtggaa caaaatctgt
     9961 ttgcgatcgg ggaagatttc catcgtggac gtcatgtacc ccacggggag gaaggatcgt
    10021 actgaggccc cccgtcgatc ctccgagtga gtcaggattt cgtagggatt ggcaggccct
    10081 tctggtccaa acggcttcgt cgtggccaca cagtgagcgt aaatgatctc ccgcttggaa
    10141 gtgtcaatga cggggtcaga gatgtacccc gggcgcccgg ccaagtgatt aaccatgacc
    10201 attgtgacgg tcgaagggag gtcggcttcg caagcgccaa caaatccgct attgagcagc
    10261 tcgacaaacc caagacacgg gtaggccttg agttttccgc tataaaaccc acctaagcaa
    10321 ttgatagtaa tggcctcggc acgatacttg ttcaacagag ccttttgtgc caagtagttg
    10381 gccgccgaga gccttagggt ttcctcgggc tcgtcaatca agaccttctg ggcttctttg
    10441 cgccattttt cggccaactc atcgacttgg gccttgtccg ccgcttcggc agcctcggcc
    10501 atttcttcga agctgacgaa atggaccggg attccgagtt gttgctgaat ggcctctgca
    10561 acacccttat ttccgccccc aatcgagata ataccgctgt tcttgacctc ctccaaacac
    10621 tggccgaccg atttgagtgt caaacgatcc tcggctggtt tttgaatccg cggcttcggt
    10681 gtcctttcct ggcgaattcg atcacaagca gctacaaact gttcgactcc tcctgacttg
    10741 aggatggtga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgctcaatc
    10861 tccggtctca tgtcgtgtcc aatatggggc caggtgggcc gatcttgaac taatgcccag
    10921 caggcaaaaa ccagtcgcac ccgaacccga tcagaagacg caggaattgc cgaactttcc
    10981 tgcgattcag caggagcaag tcggagcgaa gacgccgcca gcgcggctac gccgcatccc
    11041 gcgcaacttt tcaagaagcg acgacggttc gcgcagcaac gacacatgag agcatcgcga
    11101 gaatgcaatt ccatgatggg acctccctta ctgatggttg tcggaactca accaacgccc
    11161 aaaatatgag agacgtgttc gcggcaggcg gccaagctgc aaagatctcc actgtacaac
    11221 gaccacccac atcgataatc ctcacccagt acaaccagtc tacacacttc agggtggcat
    11281 gactgtcaag tgacttggcc atattttgtc aaaatttttg gagaaacgca agcaagagaa
    11341 aattgggcgc gtttcaaaaa agtcaccccc aaaaggcaac cccacatgcc ccggcgagaa
    11401 acttgggccg ccgcagggct actcgtctgg gaggctctcc tggaccaagc ggctaatttt
    11461 tttgaactga tcggtaccgg ctcggacaca ccactcaaaa agggccgcct cagtcgtcgt
    11521 cagcacggcg ccggatactt ccatccgccg aagggcgatt tcgtaatcta tccgatgcct
    11581 tgacccaacg gcgtcggcgg caacatagac accccatccc tgtgcgagga aatcgaggac
    11641 ggtctgctga acgcatacgt gggtttcaat gccggttacc aagagattcc gcataccctg
    11701 tgccttcaac tcgttgaaga gattccagca ttcgcggcaa ctaaacatca gtttctccgg
    11761 tagcgtccca agacgttcgg ccaagacggg cacagtcggc cccagcccct tggggtattg
    11821 ttcggtgccc acgactcgca caccgagaat cgtggcggcg tcgatcagtc ggcgaatatt
    11881 ccagactata cgcttgtgat cggtaatgtg agggattagc cgttcttgga cgtcaatcac
    11941 cagtagtgcc gtattgtctg ctgacatcaa ttccggactg cggggcggaa ggtgttcagg
    12001 ctccatggtc atggctcatt gtttggacac ttccaaggac aatgcttcaa aaaaattgcc
    12061 ggcaacaagg cattaaacgt ggtttaagat taccggccca agaccgcggc gacaagggat
    12121 atcaagttga tctaggagct ttgggcagag acatttataa ttgccggaaa ctggacgagc
    12181 tatgttgatt gtgacgaata accacgccat cggcgaagga gcaccgatgc gatccattgc
    12241 catcatgaac caaaagggtg gggtcggcaa aacgacaaca gcggtcaatt tggccgcggc
    12301 catcgcccag gagggctaca agacgtgcct gatcgacatg gacccgcagg ctcatgcgtc
    12361 gctccatctg ggcattgagc cacaaatcga gatgctgtcc atttacgatg tgttggtccg
    12421 cgaccgccca ttggcggaag tgcgacggca gatcaatgat catctatggg ttgtgccatc
    12481 tcatctggac ctggcggcgg ccgaagtgga attggcggga gtcgtcgggc gggaggtcat
    12541 cctgcgggat aagcttcgtg aggaccccga aaagtttgac ttccttttcg tcgattgtcc
    12601 gcctagtctt ggcatcctca cgattaacgc ccttgctgcg gtcaacgagg tgtttatacc
    12661 gttgcagcca catttcttgg cgcttcatgg cctgagcaag ctgctacaga ccatcgaatt
    12721 ggtggccaag cggttgaata atcggctgcg gttgacgggc atcattctct gcatgtttga
    12781 tagcaacacc cggttagcgg ccgaggtctc ggaagatgtg ggggcatttt tcggaaatca
    12841 tcgggaaaga aaaagcccgt gggcgggggc acacatgttt caaacacgaa ttcgccgaaa
    12901 cattcggttg gcggaggcac ccagttttgg tcagtcgatc ttccagtatt cgcccgattc
    12961 aaatggtgcc gaagattacc gcaacttggc ccgggaggtg atccagcaga tgccggttgc
    13021 cgagaattcg tccttcaaaa aggcgtgttg atcgcgctgc aaagttgaca cggtcgcggc
    13081 gaacgaaaaa aaggggctcg cagttgtgtt tgttgacacc gtgggctttg tttcctggtg
    13141 cgcagtttag cctgtgacga ttcgacgaac ttttccgggg aacgaaggtt tgtcgagtta
    13201 cccgctttga cgggtgatac cgatagagta tcggcctgca atcggccatc tttagccgat
    13261 gggcgccagg tttgacaggt gggcaatgtt tttcgactaa tcttgtttgc cggtgggatt
    13321 aggcttttct tgacgattct ccctaatgca cggaacggtt acagtttgcg tgcattattg
    13381 gtttttctga aaggtccaac gaaattcagc cccgacgacc ctttatttgg cgagaatccg
    13441 tggcccgcgc gctgagttgc cgtttggcgc aaagccgcgt gactttcggt gccccgtttc
    13501 ctatatgatt tccgaagcga ctcgatgccc atgaacgtcc gattggcgtt gatatgcggg
    13561 ttggtggcgg ttgttcttct cgctaggcca atagtggctt tggggcagtt cggactgagt
    13621 gccagccctg atctgttgcc ctggctgaag tctcccccat cgtctggcat agggtttcac
    13681 aattcgcagc aaggcggcgg tggtataagt gagccccaac aggaggaagg tcccccgatg
    13741 cacaccggtt ggcttgcccc gttaaatggg ggacattccg gtctcacggc cgcgccggag
    13801 cctcgggacg ctggacgaga ggtcggtgtc gtggggcgac cagacgggag gggaatggga
    13861 gtcgtacctc ctgaaactgc cccagggcac ccactatcct cactatcgca ccaggccagc
    13921 cctggctgtt atcgggacct cctggaaatt cccacttctg gcacatccgt agcagattgc
    13981 ccaggttgct ttgattcctg cggtgaccct cagtcgtgct ggcagtggtt tattgagcct
    14041 gctgggctaa ttatgggacg gaatgatccg aaccgggtgt ggtttagcta tcagtccaac
    14101 aacaatgcta atgaactgat gcacagtcag caagcccgta cttcctggca agggggttgg
    14161 gagctaacga tccggcgaac tttcgcttgt gacaattggc ggttggaagg cacctactgg
    14221 ggaatcgcgc agatgagggg agatgcttcg gcgacacacc cggagtttgt aagcagtact
    14281 ctgatcttca cggacgtcgt ttacgccgac ccgacgattc ccggcatgcc ggaggatcta
    14341 ttctttggca catatgagca aaaactgtgg cggacgaatg aaatccacaa tgtggaagtt
    14401 aacctggttc gtcaccgcat cctttgggat attcatcgcc tgggcatcga ctggttggta
    14461 ggggtccgct atttccggtt tgacgaacga ttgacttatg gttcgcgggc tcatgatggt
    14521 ttttgggggg agtatcccga gttggagggt tacctttccg acagaattga gaacaatctg
    14581 atcgggattc aacttggctt tgatctgcag tatcccatcg gcaaatcgtt acagctcgcg
    14641 tgcaccccca agatcgggct atacaataac catatccata atcgtttcga tgcttaccgc
    14701 ggtgacgggg ccttgtttgc ccccaatccc aatcccccag tgggagaccc ggtgcccgga
    14761 aggtatcccg tgatttcgtc agatgatcgt tttgccacgc tagcgcaatt ggacgcgcgc
    14821 gttcaatggc atgtgacacc ccgttggttg atgttcatcg gctatcgcct tgtttctgtg
    14881 tctgaaattg gactggccga tgctcaattc ccatgcaacg tggtggatat tgttgatgcg
    14941 atctcccaca ttgactccaa cggcgacttg ctccttcacg gtgcgtttgc agggatgtcg
    15001 atagcctttt gagtcttgct acagaccgct gggtggctca ttgtgaattt cgcctgccga
    15061 actaaagccc tccggcgtct caccgagaaa atagacatcc cggttgggac gcgaacctgg
    15121 gcagtgaata tttagcattt ccagcacatc ctcttccgtg atgaactgat ggggcgtccc
    15181 cggcggcagt ctgagaaagt cgcctttt
//
LOCUS       DAOSUT010000094        14992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2414_length_14992_cov_8.921805, whole genome shotgun sequence.
ACCESSION   DAOSUT010000094 DAOSUT010000000
VERSION     DAOSUT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_14992_cov_8.921805"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLD05_09740"
     CDS             complement(<1..182)
                     /locus_tag="PLD05_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77764.1"
                     /translation="MSTQTLFRKGAMVALLATGFVLGFLANYLWVTGHAAEQTGQTAA
                     TGEVKRVMRVGQVIGLR"
     gene            complement(357..1964)
                     /gene="nadB"
                     /locus_tag="PLD05_09745"
     CDS             complement(357..1964)
                     /gene="nadB"
                     /locus_tag="PLD05_09745"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOP77765.1"
                     /translation="MKSVEPRYLTPFHPRQIPHFFTDVLVIGAGLAGLRSALAVDPKL
                     SVLIACKDGREGSNSWHAQGGIATVMDPEDSIENHVRDTLEAGGGPCDETVVRSVISE
                     GPERIRELISWGAEFDRVGNRLALGREGGHSHNRIVHANGDATGREIVRILYDRVRQQ
                     PNIDIWEDTFTIDLLTWEGRCRGAMVWNHRHGRTLIWAKQTILATGGCGQLYRETTNP
                     EVATGDGMALAYRAGAELRDMEFIQFHPTVLYIAGSARHLITEAMRGEGARLVDRHGH
                     RFMFDYDPRGELAPRDIVSSAIVEQMAKTRHPCVYLDLTHLDPNKIRQRFPGITKICE
                     EFRLDLTRDRIPVRPGAHYMMGGVTVDLVGRTTVPGLWAAGEVTSSGLHGANRLASNS
                     LLEAIIYGRRAGEGASEAAKQEPDDFSVLPLANAPTPSPHEPLDLVDIRNSVKSLMWR
                     SMGVRREKEGMMEALEDVERWCSYVLPRQFNHPQGWELQNMLTIARLIIQAALTREES
                     RGCHFRTDFPFPREEWQQRHITFRYQP"
     gene            complement(2086..6054)
                     /locus_tag="PLD05_09750"
     CDS             complement(2086..6054)
                     /locus_tag="PLD05_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77766.1"
                     /translation="MASNVTYPRPLPPPPPVTAGRAAQHVDREELLILQQVEATRRRI
                     ATVELTWRILVLVTGTVLFAIIAAILDQWVFPKGLPPLFRWSLWATFITAASVWTVVS
                     IVPYLVRRIHPVFAAYVVEQAVPNLKHSLISFLTLRNAREEFQSDPIRKNVFRGLEAT
                     TANTIAKVPADAVVDRSQIIRTGYVLIALTAIFCLYVIFSPKDPFRSFARALLPWSRI
                     SPPTRVKIDDVRPGTSGIFEGERVVVTANVRGLKAGEEVKLVYSTSDKQAINQAIPMV
                     RERENDDRFSCELSQSFQQETTYRILAGDATTEEYRLTVVIPVRIRIRTVEYQFPRYT
                     GLAPRVTKNLGDIRAIEGTQINMTVESDVPLSTAEMVIEKLQTVSVPMAVKGREAFAR
                     TTLRLSKPEASEPEFAAYFIRGQSADGRPSVDPVKYTIEIIPDRPPEGSWLEPKEEEV
                     NLPVNGALPLRIRAVDPDFGLRHVTLEIEAASSPVAKEGLLEKPSGESHSGEFQGEYL
                     FSPKKLNLAVGDVAICRVTLVDNREPTPNRTVLPVRRIRIVAEDAAGQSSDRQPMSQP
                     QQSRNQPSQKTPDAEKNQQRPPENPAQSQLPEEKTSTANEPQSTEGPQQPREPQEQSS
                     RPAEHPAATQDRQEGASRPTPPTENPSETGEPQKNTGAEANNPQKSDPTSGSSSPQTG
                     DQGQMGDQSSSQQGKQQTGTQSPTESSQGRETGQQSETPSGQGTSADRQGDSASTPAA
                     QQGTETGRESAGEPAKSGAGQQTDAREATSQPSADPDNRTTPSEKPGEGQQPATGSRD
                     THTPRPDGQGQTGQSEASPDRSANQGEKAGGQQQGIPTSPQTGTGSDSTASGRPQPNQ
                     GQGSGDNSAGSGERTGGDQTSGSPGVSGSPSPSEPRTTSPGTRQEQTPEQKGTPPGEK
                     VDGISNPGEAVERILSYLEKKGIDPSQLTREAKAGESEQASPREVQKGPTQGEKSRTP
                     SPTDAAGGASSPQPTPEQGGQARPGASPDMPSGVPQLTENPPASGRQDEPAAPPPMGA
                     PPGESGSPDRDPAGQGSQEPPAAGQPDSSARQNPHQPPQGVAGDQAGMGGQGGGRQSP
                     QPGEGTPGSQTPTDSQEGAPMASSEGQETGQAGAKSGASAQSNTTGQGEGGPSQEGQT
                     SQSPGTTGPADQGSRGTPSQMAGGPPSRGGMPGGTRGPGGGTPPVETPVDDPNLEFAR
                     RQTDLVLRYLEEELAKQNPDKELLGQLGWSREDAAEFLRRWKELKQAAGDPSPKGKMA
                     QKQLNEIFKSLGLKPRSSTIQGSRQHQDELRGMDAGRDVPPPPQWSEYFEAYLKSLGA
                     TP"
     gene            6583..7641
                     /locus_tag="PLD05_09755"
     CDS             6583..7641
                     /locus_tag="PLD05_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03009 domain-containing protein"
                     /protein_id="HOP77767.1"
                     /translation="MLRYGKKRLEIGKTGHPLVGHLAVLAILCLPAHVMAQGIPQGFQ
                     AQTPTGNAAPGYPNYRSQPGAQPVPGNTVSPGAVVAGQSVPPQGGNNVVAPPFVLTPA
                     EQEQLDRVLLAWEERSSKIKTFECEFTRWEFDPVWGPPNQPKRIVRGKIMYSAPDKGL
                     FRVDEEVINGRLQPAAQPERWVCDGRSIYEYRFETKQIVETPLPPELQGKTITEGPLP
                     FLFGAQAESLKRRYYMRIITPQNVTEEIWLEAFPKYQQEAANFQRAQLILKLPQLDIF
                     ALQLYHPSAQAASQSRTVFQFDRIKINAVDFLRLFKEDPFKPVLPDSSWAKVIETSKP
                     GQQPESAMGQRPTPYRAQ"
     gene            complement(7674..8660)
                     /locus_tag="PLD05_09760"
     CDS             complement(7674..8660)
                     /locus_tag="PLD05_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77768.1"
                     /translation="MRLMHIGTDEAGYGPTLGPLVITATVWEGPHHLYPNHWYDLLSP
                     FVVSAPRLRPSGTGNRDDEQAPVVITDSKRIYRSGGGLGGLERTALGLLGLAHGIPKS
                     WQELIEYVDPAALADLSITPWLADFNPPLPVAATPNQVKQAANQLLQAMDKADLRCTK
                     IRCRFITAFRFNQELERFASKSELLSHVTLRIVCELLPNQGEVILWADRHGGRAHYAN
                     VLYQAFDASFVHIEQESTDRSSYRVSRAQHQFRAVFLTKAERLLPVAAASLVSKYLRE
                     LAMLAFNRFWQRRVPGLRPTAGYPVDAQRFLAEIASIQQRLGLSADLVRRRK"
     gene            complement(8683..11133)
                     /locus_tag="PLD05_09765"
     CDS             complement(8683..11133)
                     /locus_tag="PLD05_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOP77769.1"
                     /translation="MIPSSPEVMQSGSRLPEVVIAFWVRYRHLFLTLGIALFSALVVL
                     SPRVPNSFVVTELLPPRHPERVQWERFKQIFGSDELVLVVYRDPQLFDPSGSGMARLH
                     SVTQRLRAIPGVRDIWSIDQPLGNLIIESKLTFAQDLRRFFEGYTHNSHGDLCAVVVV
                     IDGSPEATHRLTTTLLELDRVAATLPEGMLVGPPVLIHEGLRQAEADARQLLWLVAAL
                     LSIVVFAISRQIRWVIINLAIVGAAILASQATLRPSFGFASAPLASLITVVGVATLMH
                     VFTRYKLCDTFGRSREESFILSIQQLAKPVLLSLVTDCVGFGALSFSDIQPLREFALM
                     AVFGIGWLALSGVLFLPGLVLILSREKNPSGKAHPARRWTALPRQRITRFSRALLRIL
                     KITKRLWYLLIPAVLALGALSIGGLFQLDVETDFTRHFRKRNKLVVAYEVVEHELGGA
                     GVWDIAVPAPRELHPDYLRAILRLEDRLRQEVLIPCEGGRRPGLTKVLSLADVVESFR
                     QDKSLRFIRQDLMDAVILGVIRQRMPGLYRALYAPDPVVPDRWWLRIMLRSPERQDAS
                     SRRQTIKQVRDITAEEWPAIRATLRFKSSGQSEISQKDQDDDISPLVTGYFVIFSVVV
                     ESMLRNQNITFLGALVGMICVFWLAFRRIWTVLALILCNTLPIIFILGALGWCRIPVN
                     FGVVMMAAISLGLSVDSSLHYATTYERLLKAGISPIRSIFCCQMLLGPPVIYATLSLC
                     VGFLSLTTSQLVPTAVLGILLSFTMLSGLLTNVTLLPWLLLITPRNKNCSNEGTMPAS
                     NDRKTQNEIVMDKLEGDS"
     gene            complement(11171..12685)
                     /locus_tag="PLD05_09770"
     CDS             complement(11171..12685)
                     /locus_tag="PLD05_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOP77770.1"
                     /translation="MNGKSTEFVKIASLRSIAEFQEHIASLGYELPVDEQILSAAESS
                     PLAQPIEVGGYRIGNRWCVHPMEGWDGTPDGQPSKHTIRRWEHFGQSGAKLIWGGEAF
                     AVREDGRANPNQLFCRPENKEAMRQLLSHLKDAHRERFGAHALDDLLVGLQLTHSGRF
                     CRPRRKDLPEPLIAYHHPVLDQKFGIDPTNDNVVLKDEDIPPLIDAYVEAAKMAEEVG
                     FQFVDVKACHGYLGHEFLSAFDRPGPYGGDLMGRSRFLREIITAIRSECPRLMIGVRM
                     SIFDFPPFRPDPNQSREGKLGPGIPHDYPIPYPGFGCDRQNPLAIDLREPIELLRLLH
                     RDYGVMLVNLTAGSPYYNPHIQRPALYPPSDGYQPPEDPLVGCLRQIHAVKQIKQAIP
                     QLVVVGSAYTYFQEFLPQVAQGVIRAGWTDFVGIGRLVLAYWDLPADTLAGRPMQTKR
                     LCRTFSDCTTGPRNGLISGCYPLDPYYKELPEFQALRSFKSAMRAKSGDSSESS"
     gene            complement(13089..13283)
                     /locus_tag="PLD05_09775"
     CDS             complement(13089..13283)
                     /locus_tag="PLD05_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77771.1"
                     /translation="MTEKRLDRRDKRRLESLRAQLQDLQRRLAGAKKQPDDPREIQQL
                     EQQIKAVQAEIDRITSQGSR"
     gene            complement(13331..14623)
                     /locus_tag="PLD05_09780"
     CDS             complement(13331..14623)
                     /locus_tag="PLD05_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOP77772.1"
                     /translation="MSAPDPQKQREFAVWVVRKLQERGYIAYWAGGCVRDQLLGRVPK
                     DYDVATNATPDEVRNIFGMKRTFAVGAAFGVITVNGPPGAGQIEVATFRTDSSYRDGR
                     HPESVSFSSPKEDALRRDFTVNGLFYDPIQDRTVDYVGGVEDIKRRIIRAIGDPYQRF
                     TEDKLRMLRAIRFVAELEFDLDPRTYEAIRAMADQITQVSQERILMELERLLTAPARR
                     KGTILLRESGLLRVVLPELEPRDNAQEQQWMTNLDILAKLKEPGFPLAFAVVARGLVE
                     PQHVTSLCRRLRMSNQQSDKIQWLLEHERSLEGAEKKRWSEVQPILISPHIDDLLNWM
                     EARAEVENRLSNSALWCRERLNWPKEQLNPPPLIRGDDLLETGVVQGPMFREILDRVR
                     KAQLDGIVTTREEALQFGLGLAKNYDLNKNTQRSGNGR"
     gene            14909..14992
                     /locus_tag="PLD05_09785"
     tRNA            14909..14992
                     /locus_tag="PLD05_09785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14943..14945,aa:Leu,seq:cag)
BASE COUNT         3250 a         4229 c         4178 g         3335 t
ORIGIN      
        1 cgtaggccga tgacctggcc gacacgcata actctcttga cttcgccggt agcggctgtc
       61 tgtcccgttt gctctgccgc gtgacccgtc acccagagat aattggcgag aaatcccaac
      121 acgaagcccg tggcaagcaa cgccaccatg gcacctttgc ggaacaaagt ttgggtactc
      181 attcgatcct ccgttgttca aaaaccaggg tcagcgaaaa cactcttttg gtgaccaacc
      241 agcttaattt gggattattc acacgcagcc tgcattgtca agcgaccgtt tctaacgtcg
      301 ctgctgccgt tgaagcgacg agcgcgggtc aaacgataac ctccgcgagc tctgtcttac
      361 ggttgatacc taaatgtgat atgccgttgt tgccactctt cacgcggaaa tgggaaatct
      421 gtgcggaaat ggcagccccg agattcttct cgggttaaag cggcttgaat gatcagtctg
      481 gcgatagtga gcatattttg caattcccag ccctgggggt gattaaactg acggggcaag
      541 acatagctgc accaacgttc cacgtcctct agggcttcca tcatgccctc tttttcccgt
      601 cgcacgccca tactgcgcca catcaagctc ttcaccgagt tgcgaatatc aacgaggtca
      661 aggggctcgt ggggcgaagg cgtgggggca ttagccagcg gcaacaccga gaaatcgtca
      721 ggctcttgtt tggcggcttc gcttgcccct tcgcctgccc gacgcccata gataatggct
      781 tcaaggagac tattggaagc caagcggtta gcaccgtgca aaccggaaga ggtgacctca
      841 cctgccgccc acaagccggg caccgttgtt cgacccacaa gatccactgt aaccccaccc
      901 atcatgtaat gggcgccggg tcggacagga atccgatcgc gggtcaaatc gaggcgaaac
      961 tcctcgcaaa tcttggtgat gccgggaaat cgttgccgaa tcttgttagg gtccaaatgc
     1021 gtcagatcaa gatagacgca cggatgccgc gtttttgcca tttgctccac gatggcgctg
     1081 ctaacaatat cgcgaggagc gagttcaccg cggggatcgt aatcgaacat gaagcggtgt
     1141 ccgtggcgat caacgagccg ggcaccttcg ccccgcatag cttccgtgat taggtgccgc
     1201 gcacttccgg ctatatagag gacggtgggg tggaactgga taaactccat atcccgcaac
     1261 tcggcccccg cccggtaggc aagggccatc ccatcgccag ttgcgacttc gggattggtg
     1321 gtttctcggt acaattgtcc gcacccaccg gttgcgagga tcgtctgctt ggcccaaatc
     1381 agtgtccgcc cgtggcggtg attccacacc atggcccctc gacatcggcc ttcccacgtc
     1441 aacaggtcaa tggtgaatgt atcctcccaa atgtcgatat ttggttgctg gcgaacccgg
     1501 tcgtagagaa tccgaacaat ctcccggccg gtggcatcgc cattggcgtg gacgatccga
     1561 ttatggctat ggccaccttc tcgtcccagg gctagtcggt tcccgacacg atcgaattcc
     1621 gcaccccagg agatcaactc ccggatccgt tctgggccct cggagatgac gctccgcaca
     1681 accgtctcat cacaaggccc tccgccagct tcaagcgtat cgcggacgtg attctcaata
     1741 gaatcctctg gatccataac cgtggcaatt ccgccctgcg catgccaact attggagccc
     1801 tcacgaccat ctttgcaggc gattagaacc gacaacttgg gatcaacagc tagagcagac
     1861 cggagtcctg ccagtcccgc accgatcaca agaacgtccg taaaaaaatg cgggatctga
     1921 cgcggatgga agggcgttag gtaccggggc tcgaccgatt tcataaaaga ctcctcgcac
     1981 gctggtttcc ataatgacgt tgccagtggg aaccgacacc agggttatct gggtggtttg
     2041 agtggcatcc tcggatgatc accggagata gccacggaag acagcctacg gtgtcgctcc
     2101 gagcgacttc aagtaggcct cgaaatactc actccattgg ggagggggag gaacgtcccg
     2161 accagcatcc attccacgca actcatcttg gtgttgccga cttccctgga ttgtactact
     2221 tctcggcttc aatccaaggc tcttaaagat ctcgttaagt tgcttttgtg ccattttgcc
     2281 ttttggcgag ggatcccccg ctgcctgctt caattctttc catcgacgca ggaattcggc
     2341 agcgtcttcg cggctccatc ctagttgtcc gagcaattcc ttgtcgggat tttgttttgc
     2401 caattcttcc tccagatagc ggaggaccaa atcggtttgt cttcgcgcga actccagatt
     2461 gggatcatcc actggtgtct ccacaggggg cgtaccacca cctggaccac gagtgccacc
     2521 cggcattcca ccccgagaag gcggaccacc tgccatttgc gacggtgtcc ctctcgaacc
     2581 ttgatcggct ggtcctgttg ttcccggact ttgggaggtc tgtccttctt gcgaaggacc
     2641 tccctcacct tgtcccgtgg tgttcgactg agcggaagca ccggatttcg cgccagcttg
     2701 accagtttct tgtccctcgg agcttgccat gggagcgcct tcctgagagt cggtcggggt
     2761 ctgggaaccg ggcgtgccct cacccggttg aggtgattgc cgtccaccgc cctgaccgcc
     2821 catacccgcc tgatcgcccg caacgccttg tggcggttga tgggggtttt gtctggcgga
     2881 actgtctggc tggccagccg ccggaggttc ctgcgacccc tgtccggcag gatcacggtc
     2941 tggcgatccg ctttcccccg gaggggcacc catgggtgga ggtgcggcag gctcatcttg
     3001 acgcccggat gctggtggat tttcagtcaa ctgcggcacc ccggacggca tatccggact
     3061 ggccccgggc cgtgcttggc caccttgctc gggtgttggt tgcggagaac tcgcacctcc
     3121 cgcagcatct gtcggcgatg gtgtccgtga tttctctcct tgagtggggc ctttttgcac
     3181 ctctcgcggt gaggcttgtt cgctttcccc ggcttttgct tcccgggtca actgagaggg
     3241 atcaatcccc ttcttctcca ggtaagaaag aattctctcc actgcttccc caggattgct
     3301 gatgccatca actttttccc cgggaggagt gcccttttgc tcaggcgttt gttcctgtct
     3361 tgtcccaggg gaggttgtcc tcggctcaga cggcgagggg gaaccactca caccgggcga
     3421 gccggaggtc tggtcaccgc cagtccgctc gcctgaaccc gcagaattgt ctccgcttcc
     3481 ctgaccttga ttaggttgag gacgtccgga tgcggtggaa tccgagccgg tccctgtttg
     3541 aggactcgtt ggaattcctt gttgctgtcc gccggccttt tcaccctgat ttgcagaacg
     3601 atcgggcgat gcctcgcttt gaccggtttg gccttgaccg tcgggacgcg gcgtgtgagt
     3661 atctcgcgaa cccgttgccg gttgttggcc ttcgccaggt ttttccgatg gcgtggtccg
     3721 gttgtccgga tccgcggagg gctgactagt cgcctcgcgt gcatctgttt gctggcctgc
     3781 cccactcttt gctggttcgc cagcgctctc gcgacctgtt tcggttcctt gttgagccgc
     3841 gggagttgat gccgaatcgc cttggcgatc ggcggatgtc ccttgaccgg agggcgtttc
     3901 ggactgttgg cctgtctcac ggccctgtga agactcagtt ggagactgtg tcccagtctg
     3961 ctgcttgcct tgttgtgaag acgactgatc gcccatttgg ccttgatcac cggtttgcgg
     4021 agacgatgac ccgctcgtcg ggtcagattt ctggggatta ttggcctctg ccccggtatt
     4081 cttttgcggt tcgccagttt cgctgggatt ctctgtcggc ggcgttggtc gcgaggcccc
     4141 ctcttggcga tcttgagttg ccgcgggatg ctcagccggg cgtgacgact gttcttgtgg
     4201 ctcgcggggc tgctggggac cttccgtgga ctggggctca tttgccgtcg atgttttttc
     4261 ttccggtaac tgcgactgag cagggttttc aggaggacgc tgctgattct tttcagcatc
     4321 gggcgtcttc tgcgatggct gattccgcga ttgttgcggt tgtgacatgg gctgccgatc
     4381 gcttgactgg cctgccgcgt cctccgccac aatacgaatc cgacgaacgg gcaggacggt
     4441 acgatttggt gtgggttccc gattatccac aagagtcacc cgacaaatgg ccacgtcccc
     4501 cacggccaag ttgagcttct tgggcgagaa tagatattcg ccctgaaatt cgccgctgtg
     4561 actttcccca cttggctttt cgaggagtcc ttctttcgct acgggagatg acgctgcctc
     4621 gatttccagc gtgacgtgac ggagtccgaa gtcgggatct actgcccgga tacgcaacgg
     4681 tagagccccg ttcactggga gattgacttc ttcttcctta ggttccaacc aggatccttc
     4741 gggcggtcga tcggggataa tctcgatagt gtatttgacg ggatcgacgc taggacgccc
     4801 atcggcactt tggccgcgaa tgaagtaggc ggcgaactca ggctcacttg cttcaggttt
     4861 ggaaagccgg agggtcgttc gggcaaaggc ctcgcggccc ttgacggcca tggggacact
     4921 tacggtctgg agtttctcaa tcaccatttc ggcagtactc agggggacat cgctttcgac
     4981 cgtcatgttg atctgggtcc cctcgatggc ccgaatatcc cccaggttct tggtcacacg
     5041 gggtgccagg ccggtatatc gtggaaattg gtattccaca gtgcgaatgc ggatccgcac
     5101 cgggatgacc acagtcagcc ggtattcctc cgtagttgca tcaccggcca aaatgcggta
     5161 agttgtctcc tgctggaagc tctgactcaa ttcgcacgag aagcggtcat cgttctcgcg
     5221 ctctcggacc atgggaatgg cctggttaat ggcctgcttg tctgatgtcg aatagacaag
     5281 tttgacctct tctcctgcct ttagaccgcg gacgttagca gtgacgacga cgcgttcgcc
     5341 ttcgaaaatg cctgaggttc ccggccgaac gtcgtcaatc ttcactctcg tgggcggaga
     5401 tatgcggctc caaggcagta aagcacgggc gaaggaccgg aaaggatcct tcggcgaaaa
     5461 gatcacatat aggcagaaga tggctgtcaa ggcaattagc acatacccag tccggatgat
     5521 ctgcgaacgg tcaacgacgg catcggcagg taccttggca attgtgttgg ccgtcgttgc
     5581 ctccagtccg cgaaagacat ttttgcgaat agggtcagac tgaaattcct cccttgcgtt
     5641 gcggagggtc aaaaagctga tgagactatg tttcagattg ggcactgcct gttcgaccac
     5701 gtaagccgcg aagacagggt gaatgcgacg caccagatac ggcacgatcg aaacgacggt
     5761 ccaaacagac gctgccgtga taaacgttgc ccaaagtgac catcgaaaca agggcggtaa
     5821 tcccttgggg aacacccatt gatctaagat ggccgcaatg atggcaaaaa ggaccgtacc
     5881 ggtcacgaga accaaaatcc gccacgttag ctccacggtg gcgatccggc gtcgcgtggc
     5941 ctccacctgc tgaaggataa gcaactcctc gcgatccaca tgctgagctg cacgccccgc
     6001 agtgacggga ggtgggggtg gaagaggtcg cggataggta acattactgg ccatgattaa
     6061 cctgtattgt gtaatcgctc cgaccgtgcc gcgtgggcgt agcctccaga acgatgagaa
     6121 gaggcaaacc gaatgccacc gtctcgcatc tacttgtaat tatagccaaa atggggccag
     6181 gcgtagttcc gccgggtgtc cacgcggaga tgcaaaattg gtacccgtcg atgtgggaga
     6241 cgcgccgtgc cgtcacatct tcaagacaac gcagttgtag tcctttgcgc atctagtgac
     6301 cgcggattgt ggttcacctt tattccgtaa gcggaacgga cgtgaatccg gtgtgtggtc
     6361 aataatcaga gagctacccc tattttgctc ttggtggttc tgaacctgga cttctaaaat
     6421 acggaaaatc ctgggattcc cctgcctttg tccagggaga aacaggccct gattggcttg
     6481 gaggcggtga tctgggcgga atgcgaaagc cgggtagggg ctcgcctccg actggatccc
     6541 gcataatgcg tggaaagtaa ctgaattgcg gagacacaac gcatgcttcg atatggcaag
     6601 aaacgcctag aaatcggcaa aactggccac ccgctcgttg gtcatctcgc ggtccttgcc
     6661 atcctctgtt tgcctgcaca tgtcatggca caggggattc cgcagggatt tcaggcacaa
     6721 acaccgacgg gaaatgcggc tccaggttac ccaaactatc gatctcaacc aggagcgcag
     6781 ccggtgccgg gcaatacagt ttcgcccggg gctgttgtgg cggggcagag cgttccgcca
     6841 caaggcggca ataacgtcgt tgccccccct tttgtgctta ctcctgctga gcaagagcag
     6901 ctcgatcgcg tcttgctggc ctgggaggag cgcagttcca aaatcaagac cttcgaatgt
     6961 gaattcaccc gctgggagtt tgatcccgtg tggggacctc ccaatcaacc gaaacgtatc
     7021 gtgcggggta agatcatgta ctccgcaccg gacaaggggc tattccgcgt ggatgaggag
     7081 gttatcaatg gtcgactgca acccgccgca caacctgagc gttgggtgtg cgatggccga
     7141 tctatatatg agtaccgatt tgagacaaag caaatcgtcg aaacaccact cccgccagag
     7201 ttgcagggaa agacgatcac cgaaggaccc ttgccgtttc tttttggggc gcaagcggag
     7261 tcactcaagc ggcggtatta catgcggatc atcacgccgc agaacgtcac ggaagagatt
     7321 tggttggaag cctttcctaa gtatcagcag gaggcggcca actttcagcg agcacaattg
     7381 atcttgaaac ttccacaact cgatattttc gccctccagc tttaccaccc cagtgcccaa
     7441 gctgccagcc aatcgcgaac ggtgtttcaa tttgaccgca ttaaaatcaa cgccgtcgat
     7501 ttcttgcgcc tctttaagga agatcccttc aaacctgtgt tgccagattc atcctgggcc
     7561 aaggtgatcg aaacgagtaa acccggccag cagccagaat ctgccatggg gcaacgacca
     7621 actccgtatc gggcgcaatg atgaagccgg tgtttgttcg ggattgggta cggctatttg
     7681 cggcggcgga cgaggtccgc agaaagacct agtctttgct gaatagaggc aatctcggct
     7741 aagaaacgct gcgcatccac gggatagccc gccgtgggcc gaaggccagg aacgcgacgc
     7801 tgccaaaaac gattgaaggc cagcatagcc agttctcgca ggtattttga gacgagggag
     7861 gccgcggcaa ccggcaagag ccgctcggcc ttcgtgagaa agacagccct gaactggtgt
     7921 tgggcgcggg acactcgata ggagcttcga tctgtggatt cttgctcgat atgaacaaag
     7981 ctggcgtcga aggcctgata cagaacgttg gcgtaatggg cccgtccgcc gtgtcgatct
     8041 gcccaaagga tgacctcccc ttggttggga aggagttcac acacaatccg aagggtcaca
     8101 tgggaaagca actccgattt tgacgcgaag cgttccagtt cctgattaaa tcgaaaggcc
     8161 gtgataaatc ggcagcgaat cttcgtgcac cgaaggtcgg ctttgtccat ggcctgtaaa
     8221 agttgatttg cagcctgctt gacctggttg ggggttgcag ccacgggtag cggcgggttg
     8281 aaatctgcta accagggcgt tatcgaaaga tccgcaagcg cggcagggtc aacatactca
     8341 ataagctcct gccagctttt tggaatgcca tgggccaacc caaggagacc caaagccgtg
     8401 cgttcaagac cgcccaaacc gccaccagag cggtagattc gtttggaatc cgtgataacg
     8461 acgggggctt gctcgtcatc tctattgccg gttccactgg gccgaagccg aggggcactg
     8521 accacgaaag gcgaaagcag gtcataccag tgattggggt aaagatggtg aggtccttcc
     8581 cataccgtgg ccgtgatgac aagggggcca agcgtggggc cgtaaccggc ctcatctgtt
     8641 ccaatatgca tcagacgcat aatgtctctc caaaaagggc gattacgaat cgccttctaa
     8701 cttgtccatt actatctcat tttgtgtctt tctatcattc gaagcaggca tggtgccttc
     8761 gttagaacag ttcttgtttc gcggagtgat caacagaagc cacggcaaga gcgttacgtt
     8821 tgtgagcagg ccactgagca tggtaaaact cagaaggatt cccaagaccg cggttggcac
     8881 aagttgactg gtcgtcaaac tgagaaagcc aacgcacaaa ctcagcgttg cgtagatgac
     8941 aggcggaccc agaagcattt ggcaacagaa aattgacctt atggggctaa ttcccgcctt
     9001 aagtagcctc tcgtatgtcg tggcgtaatg gagcgaggag tccacactta aacccaagga
     9061 gatggctgcc atcatcacca cgccaaagtt gacgggtatc cggcaccatc ccagcgcacc
     9121 gagaataaaa ataatgggaa gcgtattaca caggataagt gccaacacgg tccagatacg
     9181 gcgaaaggcc aaccaaaaga cacagatcat ccccacgagc gcaccaagaa atgtgatatt
     9241 ttgatttcgg agcatgcttt ccacaacgac agagaagata acaaaatagc cggtcacaag
     9301 tggcgagatg tcatcatcct gatccttttg ggagatttcc gattgccccg aagatttgaa
     9361 acgtagtgta gccctgatcg ccggccattc ttcagcagtg atgtcccgaa cctgtttaat
     9421 tgtttggcgt cttgacgagg catcttgccg ttcgggtgag cgaagcataa tccgcaacca
     9481 ccaccgatcg ggaacaaccg ggtcaggcgc gtagagcgcg cggtagaggc ccggcatgcg
     9541 ttgtcggatc acgccgagga taaccgcgtc cataaggtcc tggcgaatga atcgtaatga
     9601 cttatcttga cggaaacttt cgacgacgtc ggccagactg agaaccttag tcagccccgg
     9661 ccgccgacca ccttcacagg gtatcaggac ctcctgccgc agtcggtctt ccaaacgcag
     9721 aattgcccgc agatagtcgg ggtgaagctc tcgcggcgct ggaacggcga tgtcccagac
     9781 cccggcaccg cccagctcgt gttcgaccac ttcatatgct acgaccagtt tgttcctctt
     9841 acgaaagtgc cgcgtaaaat cggtttctac atcaagttgg aataacccgc ctatcgaaag
     9901 tgccccgagt gctaagactg ctgggatcag caaataccaa agtcgcttag tgatcttcag
     9961 aattctcaac aaggcccgtg aaaaccgagt tatgcgctgg cggggaaggg cggtccaccg
    10021 ccgtgcgggg tgcgcttttc cggaagggtt cttctcgcga gataatatca gaaccaaccc
    10081 gggcaaaaag agcaccccgc ttagcgcgag ccacccaatc ccaaagacgg ccatgagggc
    10141 gaactcgcga agtggttgga tgtccgaaaa actgagtgcc ccgaatccca cacagtcagt
    10201 tacaagcgac agcaacacgg gtttggccaa ctgctgaata ctcaggatga aggactcttc
    10261 tcggctcctg ccaaatgtat cgcataattt gtatctggta aaaacgtgca tcagcgtcgc
    10321 cacaccgacc accgtgatga gtgacgcaag aggtgcgctc gcgaagccaa aagaaggccg
    10381 caaggtggcc tggcttgcca gaatcgccgc cccaacaatg gcaagattga tgatcaccca
    10441 acggatttgg cgggaaattg caaacacgac aatgctgagt aatgccgcaa ccagccaaag
    10501 caattggcgg gcatccgctt ctgcttggcg taacccttcg tggatcaaca ccggggggcc
    10561 aaccaacatc ccctcaggca acgtggcggc gactctgtcg agttcaagta gtgtcgtggt
    10621 caaacgatga gttgcttccg gtgagccgtc gatgacaaca acaaccgcgc acaaatcacc
    10681 gtggctattg tgagtgtatc cttcaaagaa tcgacggagg tcctgggcaa acgtcagctt
    10741 tgactcgatg attaggttgc ccaagggttg atcaatactc cagatgtcgc gaacaccggg
    10801 gatcgctcgc aaccgctggg tcacgctgtg aagccgagcc attccggagc cgctagggtc
    10861 aaacagttgt ggatcccgat ataccacaag gaccagttcg tcgcttccaa agatttgttt
    10921 gaaccgttcc cactgaacgc gttctgggtg gcgcggtggc aatagttcgg tgaccacgaa
    10981 actgtttgga acccttggcg acagaacgac aagcgccgag aacaatgcga tcccgagcgt
    11041 caggaataag tgtcgatatc ggacccagaa ggcaatgacg acctccggga gacgacttcc
    11101 agattgcatg acctcgggac tactcgggat cacaaggcga gcagcctcct atctcgcgca
    11161 gttgctttga ctacgacgat tccgaagagt cacccgattt tgcccgcatc gccgacttga
    11221 aggatcggag ggcttgaaac tcgggcaact ccttgtaata ggggtcaagc ggataacacc
    11281 cactgataag cccgttgcga ggccccgtgg tacagtcgct gaaggtgcga cagagtcgct
    11341 tcgtctgcat gggacggccg gcaagggtgt cggccggcaa atcccaatac gcgaggacga
    11401 gacgtccgat tcccacaaaa tccgtccagc ctgcccgaat cactccctgc gcgacttggg
    11461 gaaggaattc ttggaaatac gtgtaggccg aacccaccac gacaagctga gggatcgcct
    11521 gtttgatctg cttgacagcg tgaatctgcc gtaaacaccc gaccagggga tcttcaggcg
    11581 gttgataacc gtccgagggg ggataaagcg cgggccgctg gatgtgtgga ttgtagtaag
    11641 gtgatcccgc cgtcagattg accaacatga ctccgtaatc ccgatggaga agacgtagca
    11701 attcaatcgg ttcgcggagg tcaatggcca gaggattctg tcggtcgcag ccgaacccgg
    11761 gataggggat aggataatcg tggggaatcc ctggcccgag ctttccttcc cgactttgat
    11821 tcgggtcagg ccgaaacggc ggaaaatcga agattgacat gcgaacaccg atcatcagcc
    11881 gtgggcattc ggaccgaatc gccgtgatga tctcccgcag aaagcgggac ctccccatca
    11941 aatcgccgcc atatggccca ggccgatcaa aggccgacaa gaactcgtgt cccaaatagc
    12001 cgtggcacgc cttcacatcg acaaactgaa agccaacctc ttccgccatc ttggcagcct
    12061 cgacataggc atcgatcaag ggcggaatat cttcatcttt gagaacgacg ttgtcattgg
    12121 ttggatcgat gccaaacttc tgatctaaca ccgggtgatg ataggcgatc agcggctctg
    12181 gcaagtcctt tcgccgtggc cgacaaaaac gaccagaatg cgtcagttgc agacccacca
    12241 aaagatcatc aagagcatgt gcaccaaacc gttcgcggtg tgcgtctttt agatgactaa
    12301 gaagctggcg catagcctcc ttattctcgg gccggcaaaa aagctgatta ggattggccc
    12361 gaccgtcctc acgaacggcg aatgcctcgc caccccagat tagcttcgcc cccgattggc
    12421 cgaaatgctc ccaccgccgt atggtgtgct tggacggctg accatcgggc gtgccgtccc
    12481 aaccttccat aggatggacg caccagcggt ttccgatgcg atacccgcca acttcgatgg
    12541 gctgcgcaag cggggatgac tcagccgccg ataggatctg ctcatccacc ggaagctcat
    12601 accccaaaga cgctatgtgc tcttgaaact ccgcaatgga tcgcagtgag gcaattttga
    12661 caaactcggt cgattttccg ttcatcgctc atccttattc aagtcggact gtgcaactgc
    12721 tcggtcaacg ccctatctcg tttccacgaa agcaggcaac ctcggtgcaa ataccggtat
    12781 ccaagggcca aagatcgttt tggccgcaga ccttgtccat ttatcagccc aagctgtcca
    12841 acttctcacg ctattatctt tgcaaattct gtggagtgca cctatcgcgc tgggttgcct
    12901 tgcgggttat ctttcgtagc gattgctccc atgacaaagc tcagggctca gacggtactc
    12961 tcgagattca cttattcgcg ggtacttccg gaagatcgtc ccacaccgcc gacccttttt
    13021 ccacactgta ggccaaattg ctccgctcgt gtttcgatct tcggaacggg cggggctacg
    13081 caaccaccct accgggaccc ctgcgaagtg atacggtcga tctccgcctg gacggctttg
    13141 atctgttgtt ccaactgctg aatttcccga ggatcatccg gttgtttttt ggcacctgct
    13201 agtcgacgct gtaagtcttg aagctgtgcc cgcagggatt ccaaacggcg tttatcacgt
    13261 cgatcgagcc ttttctccgt cacgatttca tcctccacgt tttccagttg taaactggtg
    13321 gtcgatgtgc ttatcggccg ttcccggacc gctgagtatt tttgttgagg tcgtagtttt
    13381 tggccagccc aaggccaaat tgtaaggcct cttcacgagt tgtaacgatg ccatccagtt
    13441 gggccttacg aacccggtct aggatttccc gaaacattgg cccttgaacc acgcctgttt
    13501 ccaacagatc atcgccacgg atgagaggtg gcggattcaa ttgctccttt ggccaattta
    13561 atctttcgcg acaccagagc gcgctgttgg acagccggtt ttcgacctcc gcacgcgctt
    13621 ccatccaatt aagcaaatcg tcgatgtgtg gcgatatcag gatcggctgg acctcggacc
    13681 atcgcttttt ttctgccccc tccaatgatc gttcgtgctc aagaagccac tggattttgt
    13741 cactttgctg attcgacatc cttaagcggc ggcacaacga tgtgacgtgc tgcggttcga
    13801 ccaatccccg ggccaccacg gcaaaagcta agggaaaacc gggttccttc aacttggcaa
    13861 ggatgtcaag attcgtcatc cattgctgtt cctgggcatt gtcccgcggt tcgagttctg
    13921 gcaagaccac acgcaatagg cctgactccc gcaggagtat cgtacctttc cggcgtgccg
    13981 gggccgtgag tagccgttca agctccatga ggatccgttc ctggctgacc tgcgtgattt
    14041 ggtctgccat agcacgaatg gcctcgtagg tgcgcgggtc aaggtcgaac tcgagctctg
    14101 ctacaaagcg aatggcgcga agcatcctaa gcttgtcttc tgtgaatctc tggtagggat
    14161 cgccgatggc cctaatgatt cgacgcttga tgtcttcgac cccgccaaca taatcgaccg
    14221 tccgatcctg gataggatcg tagaagagcc cgttaacagt gaaatctcgg cggagagcat
    14281 cttccttggg cgaggaaaag gacacgcttt ccggatgccg gccgtcccgg tagctgctgt
    14341 cagtgcgaaa agtggcgacc tcgatttgcc ccgccccggg gggaccgttg accgtaatca
    14401 ccccaaacgc ggcacccacg gcaaaggttc gcttcatccc aaagatattg cggacctcgt
    14461 cgggagtagc attggttgcc acgtcataat ctttgggaac ccgtcctaac aattgatcgc
    14521 ggacacaacc acctgcccaa tacgcgatgt aaccccgttc ctggagtttc cgcaccaccc
    14581 agacggcaaa ttcacgttgt ttttggggat ccggagccga catgaatgct ctcgctagaa
    14641 aaattaacgg tctcagaaaa ccggctagcc gggtaacacg cggataaaac ccccgggtca
    14701 ggccaaaggc cgcgttggca gtcgggttga cttcccgtct ctgcgttgcc cgtgtaaaat
    14761 acatttgcta tttgcgattg ttggttgcgg tagcaaaaat cacaatgggt gccgcaacgg
    14821 cgcgatgagc ttttgtcggc gatcgatgag gcacgtccta agatcgcgtg cccgatctga
    14881 gcgagaacag agtaggtttt tgccgattgc cctggtggcg gaactggcag acgcgcatgg
    14941 ctcaggacca tgtgggggta accccttgga ggttcgaatc ctctccaggg ca
//
LOCUS       DAOSUT010000095        14856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2439_length_14856_cov_9.188771, whole genome shotgun sequence.
ACCESSION   DAOSUT010000095 DAOSUT010000000
VERSION     DAOSUT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_14856_cov_9.188771"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLD05_09790"
     CDS             complement(<1..690)
                     /locus_tag="PLD05_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77773.1"
                     /translation="MRWVHACGIFGTIVASLVYASPFEWAPEDYKPWSTEFPYQRNVD
                     LTFQVTPIRIPDGGPGDPGIPGAHYEGTADPILMLSDFVEFQGDFTWYDSVSGFPFQG
                     LIGIDNRNGNLPKGGSVVFHLDNFPIPNTEKKIWIEWDFLISAANVPISFYVSDSNGN
                     LAVEQWFGPIRTTQEGLSRQNIWFVLKPNPIWEEITILFPFVPAGGYALIDRFHVATE
                     CIPEPASGLLAL"
     gene            1283..2179
                     /locus_tag="PLD05_09795"
     CDS             1283..2179
                     /locus_tag="PLD05_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP77774.1"
                     /translation="MTSQRVVRACVVVFGLLAFPARVWAEGFPTLVLWSTVKVMAPGS
                     TATGFLVRSPSGEGVILVSADHVFRAMKADECTVLLHLRDESGKLTKNPQKLSVRQGG
                     QPLWVKHPKADVAAISVPFGPEQNLAVLPWEALATAEELLQMELQPGDLVRSVGYPHG
                     NMFKGNDLEFAVVRLGCLATHPFPPSEQASTFLIDSNTFEGDSGGPVFVWKLPGASGL
                     HREDNGWEKIIGLIVAQHFVDEQYTLIYQSGKFRHRMGLAIVLPSVTIRETVEQAIRD
                     RESKKQPDTPKPQEPAEVTSES"
     gene            complement(2218..5064)
                     /locus_tag="PLD05_09800"
     CDS             complement(2218..5064)
                     /locus_tag="PLD05_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77775.1"
                     /translation="MTPAFDPYHVWLGIPPHEQPPNHYRLLGIPCFEDDPEIIEQAVE
                     RQLAFLRQQRTTPYREVAERLEKEIQAARRILLDPEKKAAYDQSLRGPVSSHIGGVDG
                     RQDSISVQEMRSSPGGIRLFRLLASPILFLWNHPAFTSLLAACIVLSAAIVVAFTTLS
                     ERVHPEPDQIAVAVGDASRPTPSPSAPSTAPADLQRTPQAQQSERSTPAQGPQTISQV
                     PAAPQRPALAGGQPSDSSLTQMEPLAPGLIGRLAVNGEDVGAILRYIPGESVSQAALD
                     KLIAQYGLTPGEQRLVLSGVIRVQPPQGQPTLAIRMTLTADPNAVADISVQIDGRPIS
                     FPATSQEGVSHVGSVLARGDHPIVCQFQGKDLGDTFRLDIAALALPQMPQTGVAASSV
                     KDESLIVGYTAQDLAKVNELPTLGHHALNDELSTLAANELTPGHVAEGTPPPTETPSP
                     PTALAQQGGQPVPPQLDLLRAREEIRAKYRKDLQEATAPAARALLARTMLKEGNAERD
                     PARQYMLYEEARELAIAAGDPDVAIQAGEAIGLAFDVDPWPMHIAAVNQLSKTARTPQ
                     TRENVLGILQPLCDSAIAEDRYDVADSLLDLAINLAGQLRDPVRREQFSRQREEAKRM
                     AAAFEAIQRPLELLKENPDDPQANEAVGRFYCFVKHAWKKGLPYLAKAETVLLRTVAQ
                     SELNRQIGTAARLQLADDWWALGETLSEAEKAAVRRHAGYYYLQCAGLVSGEAAQRVN
                     TRLAESGRLTDLLGLAAARRATIVGNWQIGPKSLVSPPEPMTQIAFPVIPPAEYDLLV
                     EIQSVPLPPPRGQGGSQRPDPNLDLGRGAFAVGLIQQRRQFLAVTDFQVPNQGLFSFL
                     ADYDKRGPDPANPTFRPMSVIRTQRPTLIIYSVRKSDMTVTANGMPIIQYSGDYSQLS
                     MPQGWNSSNQPRIFFATRLAVYRITRAELRDVTE"
     gene            5800..7707
                     /locus_tag="PLD05_09805"
     CDS             5800..7707
                     /locus_tag="PLD05_09805"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOP77776.1"
                     /translation="MAIDLESEIGSRLAGPPALLEVLREAPRVLIPEKTSELFDLAVG
                     GPDQDYFEVAYDVPGMGSVVEAIVVRCRNGVAVNYPDPYMRRRDPDCLVVADDRPTDK
                     PRFCERFSIPFEQARAEILGWLKRQQLVVVPFFAGGKRWGFPAALVAPMNASFFAAGL
                     ALLQEMIPAGELPQDFSPQAVVYLAPPFRHTLCEGKQVVIHHRGPPVYEVFSLNLYPG
                     PSAKKGVYGVLLSLGEEQGWVTLHGSTVQVMTPYDNIMTIMHEGASGGGKSEMLEYPH
                     REPDGRLLLGQNVVTGQKRFISLIQGCTLFPVTDDMALCHPELQNTRGRKLVVSDAET
                     AWFVRVDHIREYGTDPYLERICTMTKEPVVFLNIHAVPRATCLIWEHVEDAPGKPCPN
                     PRVILPRHLVPNVINGPVAVDVRSFGVRTPPCTKEHPSYGILGMMHVLPPALAWLWRM
                     VSPRGHANPSITESDSLQSEGIGSYWPFATGCRVDQANILLRQMLETPETRFVLVPNQ
                     HIGAWKVGFMPEWLVREYLARRGGARFRPEQLKPARCPLLGYALSFMQIEGTQLPSWF
                     LEVDTQPEVGPEAYDQGAEILYRFFHKALTELAGMPGLDPLGRRIIRACLERAPLSTY
                     EALIPFNPYAS"
     gene            complement(7799..8689)
                     /locus_tag="PLD05_09810"
     CDS             complement(7799..8689)
                     /locus_tag="PLD05_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP77777.1"
                     /translation="MHRQGENRPEQEVVAHDLLEDLPVRRLTHKNFTIEGYSRAAFQT
                     YWRIPEFRLGFDLGGQPWSFMGTPSWFVSHTHIDHFVALPAYVSRRRMMKMEPPTIYL
                     PEPCVPLVERLLHLVSRLDRGRLPCQLVPVTPGQEFELSRELVVSSLATQHTVPSLGF
                     IVWERRKKLKPEFTGLPGEKIRDLRLSGVEVTNEIRFPRVAYLGDTRIDVLDEYPDLY
                     RAEVLIMEVTFVSPRHRGEKIRKFGHVHIDDIVARRDRFQNEVIIASHFSIRYTRKLV
                     ERLVRKALPDLLDGRLQVWL"
     gene            complement(8776..9651)
                     /locus_tag="PLD05_09815"
     CDS             complement(8776..9651)
                     /locus_tag="PLD05_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOP77778.1"
                     /translation="MMEFRVQLDVFRGPLDLLLYLVRKHEVAITEISVAVIAQQFLNF
                     LEGLESLDVDTAADYLAMASWLAELKSFELLPHAEDLPKDEIPLEPRQELVRRLLEYK
                     RYRDLAYVLEERAKEWQKQYRRLSDDLGARERNLADEPLEDVHLWDLVNAFIRVLQEH
                     ELQAGTTLVYDDTPLQVFIERVYRQLLERQQIALSELFYPGMHRSTLVGVFMACLELV
                     RFGFARIEQPTLFDEIYLYLREDRPSCFPWEQVVEKLPSPEDTPGPSEVTDAVISLMD
                     TTQVPPGQPLYDQGQ"
     gene            complement(9812..10939)
                     /locus_tag="PLD05_09820"
     CDS             complement(9812..10939)
                     /locus_tag="PLD05_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOP77779.1"
                     /translation="MLYAVILAGGSGTRLWPESRSALPKQLLPIHGSQSLLRATVVRA
                     SQFVPYERIIIATSRDLAPAVQEELSFLPREALLVEPAPRNTAPCIGLAALRLLHNDP
                     EAVMVVMPADHVIEPQEAFASDIDLAVQVIEEDPTRLVTFGIVPHYPSPSFGYIERGE
                     ALSVAPASPTMPGSSGSMEDRPRVFRAKSFREKPSIQVAEGYLRAGNFYWNAGIFVWK
                     AKTIWDLLKRHQPSVAEPLARILSSSQSPNFPQILESEFSKAEKISIDYAVMEKADNV
                     VVVEAQFRWDDVGSWRSLERLLPADNCGNVSDAERCLLLDTTGCIVRCRDPRHLVATL
                     GVDNLVIVITPDATLVARKDREEDIRKILDKIAESGWREYL"
     gene            complement(11112..11888)
                     /locus_tag="PLD05_09825"
     CDS             complement(11112..11888)
                     /locus_tag="PLD05_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HOP77780.1"
                     /translation="MKSDVFFAIVLLKDPKSVSVPKMAEFVRTHWADAPSLTELDADP
                     VVSPEGSLLMPFMVNGEPAFLVVEDKPVPEEDLELAVLTAWYWPEASQAAEEHRAFAM
                     VVLPSAPADPLEKTAIVTQLTAALAMTSQAVAVFWAKSNLIHEPEAFFNSAKHLGSGV
                     YPIELWVGFHAEPEPDHTITFFTRGMLNFGLPEIEVYNSTRDLQFIYERVFNVAHFLF
                     EHGPVIKDQETVGTNEDEQFVVTIGPSRFDARIKTMQIDM"
     gene            12136..14436
                     /locus_tag="PLD05_09830"
     CDS             12136..14436
                     /locus_tag="PLD05_09830"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOP77781.1"
                     /translation="MTSTKSQSSTIRLNVLGMHCAGCAARVERALRAVPGVDAAQVNL
                     VTQKAVVRLADPNTSPERLIQAVVDAGYKATVVEEAVATRATATSPWEIQDQEQRLRA
                     RQVVVGSVGLVVVLAVMLFPGLGEWVTPWPAIIAATVVQVYLGWGYLASAWRQGRRGG
                     VSMDTLIAIGTWTAYVAAIAETTGGFHTVHQSSHGSGLSMYFSDAVMILTFITLGKYL
                     EIRSRFRASLAIRRLMDLSPQVALVIRGQTVAEVPIDKIQRGEILRVRPGEKIPLDGS
                     VTQGQSDVDESWLTGESLPVVKQPGDRVFAGTVNLSGTILVRVTKTADETLLSQVIRL
                     VEQAQEAKPQLARLADRVVAWFVPIVLIVAGVTFVVWGPVLSQWQLAVNGLVAVLVVA
                     CPCALGLATPTAVLVASGRAASRGILVKDARAYEAAARVNVVVLDKTGTVTLGQPQLV
                     AIEAAPGVDEDRLLATAGTAEQTSLHPLAGAVLAEVRRRKLNLADVDSVEILPGVGLR
                     VQSGGHTIVVRTAGVSNEEVTQPAELSSRPLNEEKIAPMPRADRGRKDERTMIRVVVV
                     EDGQLLGTLGFADQVSETSREAINALRKRGFRVVLLTGDRAENAAQVAAEVGITEVFS
                     EMTPSEKHTLIRQLQAEGNVVAMVGDGINDAAALAEADVGMAMSAGADIAKESASIVL
                     VRRDLRAAVDMLDLGRATVRVIKRNLFWAFAYNVALIPLATGLFFPWTGVLIPPPAAA
                     ACMAASSVSVVLSSLMLGKDVRPARL"
     gene            14606..>14856
                     /locus_tag="PLD05_09835"
     CDS             14606..>14856
                     /locus_tag="PLD05_09835"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOP77782.1"
                     /translation="MDTILVTGGAGFIGSNFVRQWLAEEPGRLVNYDKLTYAGNLDSL
                     ADVFDDPRHIFIQGDICDGVTVRRVLEEYRPWAIINFAA"
BASE COUNT         3136 a         3999 c         4465 g         3256 t
ORIGIN      
        1 gagcgcgagc aatccactgg caggttcggg gatgcactcc gtggccacat ggaaccgatc
       61 aatcaacgca tagccaccgg cgggcacaaa ggggaatagg atcgtgatct cttcccatat
      121 cgggttgggt ttcagcacaa accagatgtt ctgccgtgac aatccctcct gcgttgtgcg
      181 aatgggtcca aaccactgct ctaccgcgag attcccattc gaatcgctca cataaaagga
      241 gatcggaacg ttcgcggcgg aaatgagaaa atcccactca atccaaatct tcttttccgt
      301 gttagggata gggaaattgt cgaggtggaa gacaacgctc ccacctttcg gtaggttacc
      361 gtttctgttg tcgatgccga ttaaaccctg gaagggaaaa ccgcttaccg aatcgtacca
      421 ggtgaaatcg ccctggaatt cgacgaagtc gctcagcatc aagatcgggt cggccgtgcc
      481 ctcataatga gccccaggaa tccccggatc acccggccca ccgtcaggga tccgaatagg
      541 tgtgacctga aacgtgaggt caacgttacg ctgatagggg aactcagtcg accagggctt
      601 gtagtcttcg ggtgcccatt caaatgggct agcgtacacc aaggaggcga cgatcgtccc
      661 aaaaatccca caggcgtgta cccaccgcat gatgccgctc cttccacaaa gttgaatcca
      721 cgcaaaccgc tacagggcgc gcatccccta taatcgcacc agaacacaca ttatttatag
      781 caacgcgcag atcaatgtca atcatttttt caagttggcc gggcaatatc gggttaggtt
      841 ggatactacc atagaggggg tgccgcacgc caatctgaag tctgagcccg acttccgcag
      901 tgttatacgt tcggcctgca caagcacgac gtgccgccca acagcgcatg ggcttccgac
      961 agttctcgcg agccacccgt gcaaaccagt gggcccaaag gcatgtccct gacacagctt
     1021 gggaaacgca caagtgataa ggacacactc ccgccttgcg gtgaggggac gtgatctgac
     1081 gaaatcgcag cgatttccta acggcgcgat gcattgagtg gggtaatctc cggtggttgc
     1141 cccgctttta tcaagttgga cacacgcctg cgccggcaaa atacggattt cgcttgcaca
     1201 attcgtcggc gtctggcaac aaaggaaaga aggtcacgct tctgatgcga gatggaattt
     1261 cagtttctag ggagtaaagt acgtgacaag ccaacgagtg gtgcgagcgt gtgtcgtcgt
     1321 ttttgggctt ctcgccttcc cggccagggt ttgggccgag ggattcccga ccttggtgct
     1381 ttggtccacg gtgaaggtta tggcccctgg ctctaccgct acgggatttc tggtgcgtag
     1441 tccttccggg gaaggggtga tcctggtttc cgcggatcat gtctttaggg cgatgaaggc
     1501 cgacgagtgc acggtccttt tgcacctccg cgatgaatca gggaaattga ccaaaaatcc
     1561 gcagaagttg agtgtccggc aagggggaca acccctttgg gtcaaacacc ctaaggccga
     1621 cgtagcagcc ataagtgtgc cctttgggcc ggagcaaaac ttggccgtct tgccatggga
     1681 agcgcttgcg actgccgagg aactgttgca aatggaattg cagccgggcg acctggtgcg
     1741 aagtgtcggt tatcctcatg gcaatatgtt caagggaaac gatctggagt ttgctgttgt
     1801 tcgcttggga tgtctggcca cgcatccttt tccgccctcg gaacaagcca gcacatttct
     1861 catcgattcg aacacatttg agggcgatag cggtggcccg gtctttgtgt ggaaacttcc
     1921 cggcgcctca ggcctgcacc gggaagacaa cggttgggag aagattatcg gactgattgt
     1981 ggcccaacat ttcgtggatg agcaatacac cctaatctac caaagcggta agttccgaca
     2041 tcggatggga ctggccatcg tgctgccttc cgtcacgatt cgcgagacgg ttgaacaagc
     2101 cattcgggac cgtgagtcga agaagcagcc tgatactcca aaaccacagg aaccagccga
     2161 ggttacttcg gaatcatgag tcgccagtcg aaaggctcct gtcggccata gccaagccta
     2221 ctcggtaaca tcgcgcagtt cagcgcgggt gatgcgatag acagcaagtc gtgtggcaaa
     2281 gaagattcgc ggttgattgg atgaattcca gccctgaggc atggaaagct gactatagtc
     2341 gccgctgtat tgaatgatag gcataccatt ggctgtcacg gtcatgtccg atttcctcac
     2401 actgtaaatt atcaatgtgg gacgttgagt cctgattacg gacatcggcc ggaaggttgg
     2461 attagcggga tcgggacctc tcttgtcgta gtctgccaga aaactgaaaa gaccctggtt
     2521 ggggacctga aaatccgtca cggcgaggaa ttgccgacgc tgctggatca gccccacggc
     2581 aaatgccccg cggccgagat ccaagttggg atcgggacgt tgcgaacccc cttgacctcg
     2641 cggcgggggc agcgggaccg actggatctc tacgaggagg tcatattccg ccggtgggat
     2701 gacaggaaac gcgatttggg tcatgggctc tggcggtgag accagggatt ttggaccaat
     2761 ttgccagttg ccgacaatcg ttgcgcgacg cgccgccgcc agaccgagta agtcggtcaa
     2821 gcgaccactc tccgctaggc gagtgttgac acgttgagcg gcctcacctg atacgagtcc
     2881 tgcgcactga aggtaatagt aacccgcatg ccttctcacg gccgcttttt ccgcctcaga
     2941 tagcgtttcc ccgagtgccc accaatcgtc ggccaattgt agccgggcgg ccgttcctat
     3001 ttgtcggttc agttcggatt gcgccaccgt tcgcaacagc acggtttcgg ctttggccag
     3061 gtaggggagc ccctttttcc aggcgtgttt gacaaagcag taaaagcggc caactgcctc
     3121 gtttgcctgg ggatcgtcgg gattctcttt gaggagttcc aagggacgtt ggatggcttc
     3181 aaaggcggcg gccatccttt tcgcctcctc ccgttgccga gaaaactgct cgcgacgaac
     3241 gggatcacga agttgaccgg caaggttgat ggccaggtcc aagaggctat cagccacatc
     3301 atagcgatct tcggcaattg ccgaatcgca caatggctgg aggatgccca agacattttc
     3361 gcgggtttga ggtgtccgcg ccgtcttaga aagttggttg accgccgcga tgtgcatcgg
     3421 ccaaggatca acgtcaaagg ccaggccgat agcctcaccg gcctggatcg ccacatcggg
     3481 atcgcctgcc gcaatggcca gttctcgggc ctcttcataa agcatgtact gccgcgccgg
     3541 atcacgctct gcgttcccct ctttgagcat cgttctggca aggagagcgc gcgctgcggg
     3601 cgccgtcgcc tcctgcaagt cctttcgata cttcgcccgt atctcttctc ttgccctgag
     3661 aagatccaac tgtggcggca ccggttgccc gccttgctgg gcaagtgcgg tgggcgggga
     3721 gggtgtttcg gtgggcggcg gtgtaccctc ggcaacgtgc cccggtgtca gctcatttgc
     3781 cgccagcgtg ctcagttcat cattgagggc atggtggccc agcgttggca attcgttgac
     3841 tttcgccaag tcctgtgcgg tgtatccgac aataagcgac tcgtctttta cggacgatgc
     3901 tgccacaccg gtttgaggca tttgtgggag cgccagcgcg gctatatcga gccgaaacgt
     3961 gtcgcccaag tcctttcctt gaaactggca gacgatgggg tgatctcccc gtgccaaaac
     4021 cgaaccaaca tgggagactc cttcctgcga agttgctggg aaggagatcg gacgaccatc
     4081 gatttgcacg gagatatccg cgacggcatt cgggtctgcc gtcaatgtca tgcgaatggc
     4141 tagggtgggt tggccctgcg gaggttgtac gcggatgacg cccgatagga caagccgttg
     4201 ctcgcctggc gtaagaccat attgcgcgat cagcttatcc aatgcggctt gcgaaacgct
     4261 ctcgcctggt atgtaacgaa gaatggcacc aacatcctcg ccgttgaccg caagtcgccc
     4321 aatcaggccg ggggcaagtg gctccatttg ggtcaaagag ctatctgagg gctgccctcc
     4381 agctagcgcg ggccgctgag gtgcggcagg aacttgcgag atggtctgtg ggccctgagc
     4441 cggcgttgag cgctctgact gctgggcttg tggcgttctc tgcaaatcgg ccggggcggt
     4501 gcttggggct gaaggcgagg gtgttggccg ggacgcgtcc ccaacggcaa ctgctatctg
     4561 gtctggctct gggtgtaccc tctccgacaa ggtcgtgaaa gcaaccacga tcgccgccga
     4621 cagaacgatg caggccgcca ggagacttgt gaacgccggg tgattccaaa gaaataggat
     4681 ggggctcgca agcaatcgga acagccggat gccacccgga gatgatcgca tttcctgcac
     4741 ggaaatactg tcctgtctcc catctactcc gccgatgtgg gatgacaccg gtccccgaag
     4801 cgattgatca taggccgcct tcttctcggg atcgaggagg atacgtcggg cggcttggat
     4861 ctctttttcc aaccgctcag ccacttctcg ataaggtgtt gtccgctgct ggcgcaaaaa
     4921 ggcaagctgc cgctcgaccg cctgttcgat gatctcggga tcgtcttcaa aacagggaat
     4981 acctaacaac cggtagtggt tgggcggctg ttcgtgtggc ggtatcccta accaaacgtg
     5041 atagggatcg aacgccgggg tcatgaagcc tctcctgttc cgggcaagcg gtttctcttt
     5101 ttcgtgatgg ggagggaaga ctccgtcacg aaaacgccag gcggtagcct cgggtgttgg
     5161 cgtgactacc agcgtgggtg acatggaggg ccgggcgtgc ccccacttct atggtaccca
     5221 cccaagatgc cgttgaccac acgtcccaag tgccgtttgc aacggaatgg gcatgtcaat
     5281 tttttcggta tgtgcggacc ggcacgtcgg tcatttggac tgccgcgccg gcgcctggtc
     5341 ctttatctct tcgcacgagc cgccggcgta gacgagcgga ttgagatgtc tttcgaaaaa
     5401 cgcccacctt ggtgggaagt gctgatgttt gattgcgggc gcgcgaactc ggtagggccg
     5461 acctgggtcc tgttctgttt ggccctatgg gcatacgcgg ggtaagggcc tcgtgggact
     5521 gctgccactt atagcgggat gcggatagcc tataagtgct aatgaatttg ggtggtgtcg
     5581 tcgtggagtg cccacaaatg gttaaaatcg aatatatctg aggtggtatg atcaagatag
     5641 tatgacgccc cgcacgcgtc cggaaggaat tgaccgggtg agttagccga atagttcagg
     5701 cgttgccgac gcgggcgagg gttgccggtg tgcggtttgc tttgaagcga cagttccgta
     5761 acatgccttg ggatagacgt tacgtatcga ggaacgcgta tggcgattga cctggagagt
     5821 gaaatcggga gtcggttggc cggaccgccg gcgctgctcg aggtgctgcg cgaggctccg
     5881 cgcgttttga tacccgagaa gacgagcgag ctgttcgacc tcgcggtggg tggtccggat
     5941 caagactatt ttgaagtagc ttacgacgtg ccggggatgg gttccgtggt ggaggccatt
     6001 gtggtccgtt gccgcaatgg cgtggccgtg aattatcccg acccctatat gcgacggcgg
     6061 gaccccgatt gcttggtcgt ggcggacgat cgcccgaccg acaaaccgcg tttttgtgag
     6121 agattctcta tacccttcga gcaggcccgg gcggagattc tcggttggct caagcggcag
     6181 cagttggtgg tcgttccctt ttttgcgggt ggaaagcgat ggggctttcc cgccgcgttg
     6241 gtggcgccga tgaacgcaag tttcttcgcc gctggcctgg cgctgttgca agagatgatt
     6301 cccgccggag agttgcctca agatttttca ccacaggcgg tggtttattt agcgccgcca
     6361 tttcgacaca ctttgtgtga aggaaagcag gtggtgatcc accaccgcgg tcccccggtg
     6421 tacgaggttt tctcgctcaa cttgtatccc ggtcccagcg ccaagaaagg tgtctatggg
     6481 gtgctgctct cgttgggcga agagcaaggt tgggtcacgt tgcatggctc caccgtccag
     6541 gtcatgacgc cctatgataa catcatgacg atcatgcacg agggcgcaag cggtggcggg
     6601 aagagcgaga tgctggaata tccccaccgt gaacccgatg ggcggttgtt acttggccag
     6661 aatgtcgtaa cgggacaaaa gcggtttatt tcgttgattc aagggtgtac cctctttcct
     6721 gtcacggacg acatggcgtt gtgtcatccg gaactccaga atacccgggg gcgaaagctc
     6781 gtcgtttcag atgccgaaac cgcttggttc gttcgggtgg accatattcg agaatacggc
     6841 acggacccct atctggaacg aatttgcacg atgaccaaag aaccggtggt gttcttgaac
     6901 attcatgccg tgccgcgggc cacctgtttg atctgggaac atgtggaaga tgcccccggt
     6961 aagccgtgcc ccaatccccg cgtcattctg cctcgccatt tggttcccaa tgtgatcaat
     7021 ggaccggtgg cggtggatgt gagaagcttt ggggttcgga cgccgccgtg cacaaaagag
     7081 catcccagct acgggatctt ggggatgatg catgtcttgc caccggcctt ggcgtggttg
     7141 tggcggatgg tttctccccg gggccatgcc aaccccagca ttacagaaag cgattcgctg
     7201 caaagcgaag gaattggctc atactggcca tttgccacag gttgccgcgt cgatcaggcg
     7261 aacatccttc tccgtcagat gttggagacg ccagaaacga ggtttgtcct ggttcccaat
     7321 cagcacattg gcgcatggaa ggtaggattc atgcccgaat ggctggtccg ggaatacctg
     7381 gcccggcgtg gcggcgcacg ctttcggccc gagcagctca aacccgctcg gtgtccgttg
     7441 ttgggctatg ccctttcctt catgcagatc gagggcacgc aacttcccag ttggtttttg
     7501 gaagtcgata cccagccgga ggttggacca gaggcctacg accagggggc agagatcctt
     7561 taccgctttt tccataaggc actgacggaa ttggcgggaa tgccgggatt ggatcccctc
     7621 ggtcggcgaa tcattcgcgc ctgtttggaa cgggcaccgc tgagcacata cgaggcccta
     7681 atcccgttca atccctacgc aagctgatgg tcctttgact gggcggcggt ggaatctgtc
     7741 ttggaagcag cggctcttgg cggtgaacga agccatgctg atcgctcgtt atcaaagatt
     7801 aaagccagac ttgaaggcga ccgtcgagca agtcgggtag ggccttgcgg accaaccgtt
     7861 caacgagctt ccgggtgtag cggatgctga agtgggaagc gatgatgact tcattctgga
     7921 agcggtctcg acgggcgacg atgtcgtcga tgtggacatg accgaatttg cggatctttt
     7981 ctccccgatg acgaggcgag acaaacgtga cttccatgat gaggacctcg gcgcgataaa
     8041 ggtccggata ctcatcgagg acatcgatcc gtgtatcgcc caggtaagcc acgcggggga
     8101 aacggatttc gttggtgacc tccacgccgc ttagccgtag gtcccggatt ttttcgcccg
     8161 gaaggccggt aaactccggt ttgagtttct ttcttcgttc ccagacaatg aaccccaagg
     8221 atgggaccgt atgctgggtg gccaggctgc tgacaaccag ttccctggaa agctcgaact
     8281 cttgaccagg tgtcacgggc acaagctggc agggaaggcg tccccgatcc aggcggctga
     8341 ccagatggag gagtcgttcc accaaaggga cgcaaggttc cggcaggtag atggtgggtg
     8401 gctccatttt catcatccgc cgccgggaca cataggctgg gagggcaaca aagtgatcaa
     8461 tgtgggtgtg cgacacaaac caggatggcg tccccataaa ggaccaaggc tggccgccga
     8521 ggtcaaatcc tagacggaac tcgggaatcc gccaatacgt ctggaatgcc gcccgcgaat
     8581 agccctcaat cgtgaaattc ttgtgggtca accgccgtac aggaaggtct tccaggaggt
     8641 catgggcaac gacttcctgc tccggacgat tttcaccctg tcgatgcatc tcatttccag
     8701 cagtcacaaa ccgcaaagac tcccacctaa tggccataaa gcttccaggt cttcccaccg
     8761 ggaccgatcg aaacgttatt gtccctgatc atagagaggc tgcccagggg gcacttgtgt
     8821 ggtgtccatc agagagatca cggcatctgt gacctcgctt ggtcccggtg tgtcttcggg
     8881 ggacggcagt ttctcgacga cttgctccca ggggaagcaa ctcggtcgat cctcccgcaa
     8941 gtaaagatag atctcgtcga agagtgtcgg ctgttcaatt cgtgcaaaac cgaaacgcac
     9001 cagttccaaa caggccataa aaacgcccac gagcgtcgat ctgtgcatcc cgggataaaa
     9061 gagttcactg agggcaattt gctgtcgttc gagaagctgc cgatagaccc gctcgataaa
     9121 aacctgaagc ggggtgtcgt catagacgag ggtcgtgccc gcctgcaatt catgctcttg
     9181 aaggacgcga atgaatgcgt tgacgaggtc ccaaaggtgg acgtcttcta gcggttcgtc
     9241 ggccaggttg cgctctcgcg ctcccaggtc atctgaaagc cgccgatact gtttttgcca
     9301 ctctttggcc cgttcttcca acacataggc taagtcccga tagcgcttgt actccagcaa
     9361 tctccgcaca agttcttgtc gcggctcaag ggggatttca tccttgggaa ggtcttctgc
     9421 gtggggaagc agctcgaagg actttagctc ggccagccaa ctggccatgg ccaggtaatc
     9481 agccgccgta tcgacatcca gagactcgag gccttccaag aaattgagaa actgttgggc
     9541 gatgacggcc acggagattt ccgtgatggc cacctcgtgc ttgcgaacta aataaaggag
     9601 caggtcgagt ggtccgcgga aaacatcgag ttggacgcga aattccatca tggtagttga
     9661 agggtttcgt cacgcgccac gctgcatatt tcaagttgcc cgctcgttaa tcatcggcca
     9721 cgctatgagc ctcttggagc ctctcgctca ccgaacccca cacagcccag agcgcggatg
     9781 gcgtcaacgg caggcagact attttccctg atcacagata ttccctccag cccgattctg
     9841 cgattttgtc gaggatcttg cggatgtctt cttcgcggtc ctttcgggca acgagggtgg
     9901 catctggtgt gatgacaatc accaggttat ccacgcccag ggtggccaca aggtgacggg
     9961 gatcgcggca cctcacgatg caccccgtgg tgtccaaaag gagacatcgt tccgcgtccg
    10021 agacattgcc acagttgtcc gccggaagaa gccgctctaa agatcgccaa cttccgacat
    10081 catcccatcg gaactgggct tcgaccacca caacgttgtc ggccttttcc ataacagcat
    10141 aatcgatgga gattttttct gccttggaaa actcggattc aagaatctgg ggaaagttgg
    10201 gtgactgaga agacgagaga attcgggcaa gtggctcggc cacactgggc tggtgtcgct
    10261 ttaagaggtc ccagattgtt ttcgctttcc agacaaagat ccccgcgttc cagtagaaat
    10321 tgcccgcgcg aaggtatcct tctgctacct gaatggaggg cttctcgcgg aagcttttgg
    10381 cgcgaaagac gcgaggtcgg tcttccatgc tgcccgaact gccgggcatc gtgggggacg
    10441 ctggagcaac ggacaaggcc tcgccgcgtt caatgtaccc aaaggagggg gaaggataat
    10501 ggggaacgat cccgaacgtc accagccggg taggatcttc ctcaatcact tgaactgcta
    10561 aatcgatatc ggaggcaaag gcctcttgtg gttcgatcac gtggtccgcg ggcatgacga
    10621 ccatcacggc ctccgggtcg ttatggagaa gccgcagcgc tgccaatccg atacaggggg
    10681 ccgtatttcg gggggctggt tcgacaagga gggcctcgcg gggaagaaag gacaactctt
    10741 cttgtaccgc cggggccaaa tcccggctag tcgcgatgat gatccgttcg taggggacaa
    10801 actgtgacgc gcggaccaca gtcgcccgca ggagactctg cgacccgtgg atcggcaaaa
    10861 gctgtttagg gagggcgcta cgactttccg gccaaagtcg ggttcctgag ccacccgcca
    10921 aaatgacagc ataaagcatt gtggtgtctc cctgttacca cggaatattg aggcaccctg
    10981 ggagggtgct agcgatttcc gacggcaagt cccaaatagg atatcaccat gataaaagga
    11041 gtcggtatcg cttcccaagg gtgtgttgcc ccaaaagtgg aaggctgaaa agggccggac
    11101 tgccgattgc tttacatgtc gatctgcatg gttttgattc gggcatcaaa gcgagatggc
    11161 ccgatggtta ccacaaattg ctcatcctcg tttgtcccga ccgtctcttg atccttgatg
    11221 acaggtccgt gctcgaatag gaagtgggcg acattgaata cccgctcata gataaactgt
    11281 aaatcgcggg tactgttata cacctcgatt tcgggcagtc caaaattcaa catgcctcgc
    11341 gtaaagaagg tgatggtatg atccggttcc ggttcggcgt ggaagccgac ccatagctcg
    11401 atgggataga cgcccgagcc gaggtgcttg gccgagttga agaaagcctc cggttcgtgg
    11461 atcaaattgg atttcgccca gaaaacggcc acagcctgcg aggtcatggc cagtgctgct
    11521 gtcaattgtg tgacaatcgc cgttttttcc aaaggatcgg cgggcgcgga gggaaggacc
    11581 accatggcaa acgcccgatg ttcttccgcg gcttgtgacg cttctggcca gtaccaagcc
    11641 gtgagaacag cgagttccaa atcctcctca ggcactggtt tatcttccac gaccaggaag
    11701 gcaggttcgc cgttcaccat gaagggcatg agcaagctgc cctcggggct tacgacagga
    11761 tcggcatcta actcggtgag agacggggca tctgcccaat gtgtccgcac aaattcggcc
    11821 atctttggta cagacacact tttggggtct ttgagcagga cgatggcgaa gaacacgtcc
    11881 gacttcataa agcacctctc acactaccac aaaaaacctg ttatttattg gtgcctgaag
    11941 tccagcggga cgagttttcg caacgttcct acctccaaag ccccatgggc cacaggccgc
    12001 caaaagactc ccgcatttta gggcatagat cacatttgaa caagtcccaa accctcgtct
    12061 ttgaatcacg ttttactatt atttcccata gggggccgct tgcccgccct tttttggacc
    12121 gggaaacttt gcaccatgac gtcgaccaag tcgcaatcgt caacaattcg cctgaatgtt
    12181 ttggggatgc attgtgcggg atgtgccgcg cgggtggaga gggcactccg tgcggttccc
    12241 ggcgtggatg ccgcccaggt caatctcgtc acccagaagg ccgtcgttcg gctcgctgat
    12301 cccaacacct cgccggagcg acttattcaa gcagtcgtgg atgcaggata caaagcgact
    12361 gtcgttgaag aggcggtcgc cacccgtgcc acggcaacaa gtccatggga gatccaagat
    12421 caagaacaac ggctccgcgc acgtcaggtc gtggttggct ccgtgggctt ggtcgtggtt
    12481 ctggcagtga tgctcttccc tggactgggt gaatgggtga ctccctggcc ggccattatc
    12541 gcggccacgg tggtgcaggt ctatttggga tggggatatc ttgcctcggc ctggcgacag
    12601 ggacgccgtg gcggcgtcag tatggatacg ctcatcgcca tcggcacttg gacggcttac
    12661 gtcgcggcga ttgccgagac caccggtggg tttcacacag ttcatcagtc gagccatgga
    12721 tcaggcttgt cgatgtattt cagcgatgcc gtgatgattc tgacattcat cacgttgggg
    12781 aaatacctgg aaattcgttc ccgtttccgc gcttcattgg cgatccgccg cctgatggac
    12841 ttatcccctc aagttgcgct ggtgattcga ggacaaactg tggccgaggt ccccattgat
    12901 aagatccaac ggggagagat tttacgggtt cgccccggcg agaagatccc tttggatggc
    12961 tccgtaaccc agggccagag cgacgtggat gaatcgtggc tgaccggaga atccctgcct
    13021 gtggtcaagc aacctggtga cagggtcttt gcgggaacgg tcaatctctc gggaacgatt
    13081 cttgtgcggg tgacgaagac agcggacgaa actttacttt cgcaggtcat tcggcttgtc
    13141 gagcaggccc aggaggcgaa gccacagttg gcccgactgg ccgatcgtgt tgtggcatgg
    13201 tttgtgccaa tcgttctaat tgttgcgggg gtgacttttg tcgtttgggg acctgtcttg
    13261 agtcagtggc aacttgccgt gaatggtctg gttgctgtac tcgtggtggc ttgtccgtgt
    13321 gccctcggtc tggcgacacc cacggcggtg ctcgtagcca gtggccgcgc agcttcccgg
    13381 ggaatcctgg tgaaagatgc gcgggcttat gaagcagcag cccgcgtgaa tgtcgtcgta
    13441 ctagataaga cgggcacagt tacgctcggc cagccacaac ttgttgcgat cgaagcggca
    13501 ccgggcgtgg acgaagatcg tttacttgcg accgccggta ccgcggaaca aaccagcctt
    13561 caccccttgg cgggagcggt cttggcggag gtccgccgcc gaaagctaaa tcttgccgac
    13621 gtggattcgg tggaaattct gcccggcgtg ggactgcgtg tgcaatccgg tggacacacc
    13681 atcgtcgtgc gaacagccgg ggtctccaac gaagaggtta cccagcctgc cgagctttcg
    13741 tcgagacccc tgaacgagga aaagattgcg ccgatgccga gggcggaccg tgggcgtaaa
    13801 gatgaaagga cgatgatccg cgtggtcgtg gtggaagatg ggcagttgct ggggacactg
    13861 ggatttgccg atcaagtcag cgagactagc cgcgaggcca ttaacgcctt gcgaaaaagg
    13921 ggattccgag tcgttttatt gacaggggat cgcgcagaaa acgctgccca agtagcggcc
    13981 gaagtcggca tcacggaggt gttttcggag atgacacctt ccgaaaaaca cactttgatc
    14041 aggcaacttc aagcggaagg aaacgtggtg gcgatggtgg gagacggcat caatgatgcc
    14101 gcagccctgg ccgaagccga tgtaggcatg gcgatgagtg ccggagctga catcgccaaa
    14161 gagtcggcca gcattgtcct tgttcggcgc gacctgcggg ccgcggtgga tatgcttgac
    14221 ctgggtcggg ctaccgtccg cgtgatcaaa cgcaatcttt tctgggcctt tgcctacaac
    14281 gtggcactca ttcctttggc aaccggccta ttcttcccgt ggacgggtgt cttgatccca
    14341 ccaccagcag cagcggcttg catggctgcc agttcggtct ccgtggtgtt aagcagtttg
    14401 atgttgggaa aagacgtgcg gccagcccgt ttataaggtg gaatcccccc ttttgaagag
    14461 aatgccgtga tctccccaac ggctgctgtt cagagtgaca gacaccaaag cgaggttaaa
    14521 atagatcata cgggagctga gggcgcgttc ttcgccttgt tcatgagagg cggcacatca
    14581 ggctactctc gaagcgagtg gaaccatgga cacaatcctt gtgacgggtg gtgcaggttt
    14641 catcggcagt aactttgtcc gacaatggct ggcagaggaa ccggggcgac ttgtcaacta
    14701 cgacaaattg acatatgcgg gtaatctcga ttcgctagct gatgtgttcg acgatccccg
    14761 gcatatcttc attcaaggcg atatctgtga cggtgtgacg gtccgccggg ttttagagga
    14821 gtatcgtccc tgggctatta tcaattttgc ggccga
//
LOCUS       DAOSUT010000096        14737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2458_length_14737_cov_9.109249, whole genome shotgun sequence.
ACCESSION   DAOSUT010000096 DAOSUT010000000
VERSION     DAOSUT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_14737_cov_9.109249"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLD05_09840"
     CDS             <1..1166
                     /locus_tag="PLD05_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP77783.1"
                     /translation="ENRQRFEPPSPPTPLPLNGRGEIGQYAFSRKRKMIMTCTRRSFL
                     QQGAALLGYLAAGLENRFSRGSLSLEASETVPELTSIPIVDTHQHLWDLSKIRLPWLA
                     GDERLNRNYLVEDYLEAVKGLPVVQAVYMEVAVADEDLVKEAEWIIEICRSGRGPTVA
                     AVIGGRPASDDFRTYLARFRNNPVVKGVRWIPPNSKLGHKLYFGPQMRDNLRLLGEWG
                     MSFDICIPPDWLSDAAELVDACPDTRFIIDHCGNADPQQFGRWGKERGEEATRYVEQW
                     RRAMDGLAARKNVVCKISGIIARVRPDDWRPDDLAPIVNHCLEAFGSDRVMFAGDWPV
                     CTKGAPLRAWIAALREITASRSLEERRKLFHDNAVRFYGLEPIPKFPRPLGGG"
     gene            complement(1895..2191)
                     /locus_tag="PLD05_09845"
     CDS             complement(1895..2191)
                     /locus_tag="PLD05_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77784.1"
                     /translation="MENDDPRFHYPVACQRCGVELQLGRGSCYLVKIEAMADPSPPQL
                     DFEDYPNDLNAAFRDVLEELSHLSAQEAMDQVYRRLSFYLCARCYARWIENPTG"
     gene            2460..3893
                     /locus_tag="PLD05_09850"
     CDS             2460..3893
                     /locus_tag="PLD05_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77785.1"
                     /translation="MNSVTRWLPRVGLAAALGLLSGMSLLASETAQRPNVLIITTDQQ
                     RVDAASVVGNKWLKTPHLDALAAQGVYFTKSYCSHPLCSPSRASLHAGRFPHEIGVDH
                     NNMSITPSVTISGQVFQEAGYDTGYAGKWHIPTVYPTDGIPGFTVLNNTTRQGKLAHD
                     VDDATMQVAIDFIKQKRDKPFYLVVSFINPHDICLLAGEDSPLLEQVWRKYGPPAGAE
                     LPPLPASFTLPENEPAWLLQRRPKHPQWDETHWRRYIYAYYRMVEDVDQQIGAVLKAL
                     KETGQEENTLVIFTSDHGEGLGSRRWTGKMMFFDPEAAVPLIVSWKGKTPAGRIDKEH
                     LVSTVDVLPTICDYAGIKPPENVRGQSLRPIIENPAAPWRSFIVSEIAHTPALGRSFM
                     VRTDRYKYFVIPVGQEKPLEMLFDMETDPHELKNLASDPNHSETLKEHRALLDQWKQT
                     TEEAKYPVLPNPQAARPRAQRAKQAAP"
     gene            4038..5603
                     /locus_tag="PLD05_09855"
     CDS             4038..5603
                     /locus_tag="PLD05_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77786.1"
                     /translation="MKPLFRGQQGPTVFWASMLVAIGCSYQWGQAGWATEKRPNILLI
                     EVDSMDGRVMGCMGYPAMVRATPNLDALAKRGVLFRNAYTNNPICCPSRASMWSGKYT
                     FRIEAWNNYKGLEPGTPTFRDELEKAGYLTQTFGKLDYLSGAHSVRARVTAWLRSANI
                     ERPQYRIGPPRVIEEDLVRVHAGDWRNADRAVAWLKDNAKGDKPFWLYLGLSLPHPAF
                     QTSRYYLRMIDEAGVDVPPLDQYDHSVMRYQRLVKNWEHGYSDEMIKLVRRVYFAMIA
                     ETDAIVGHVLQALRDCGLEDSTYVIFISDHGENAMEHRLWYKMNHYESSARVPMIIAG
                     PGVRQGVVEEKLVSLIDIYPTLMDMANWPKPQDLDGHSLMPELLGQPSSRPDWVLSEY
                     HDTACNTGSFMIRRGPWKYIAYPGYEPMLFNLDEDPHEIRNLAPQLPDKVREMDQLLR
                     SIVDYEAVDAKAKAYDRASFAAWREEHKKKGDYEELMAIIFSGYDGVNAAKAKPWTPE
                     DEAKVERWLKGEQ"
     gene            5637..7562
                     /locus_tag="PLD05_09860"
     CDS             5637..7562
                     /locus_tag="PLD05_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOP77787.1"
                     /translation="MTNKTNGSAATVWRFVPWRLLFWIALCSVGPLCLSGQIGFGAEQ
                     GSAGDPVEIIEAMVDAQATIRYLLAHQRENGAFGPMGHQHTDLAWNYPAARALILLEV
                     EIPRVEDCFRNGQDALYKHPGSHNTQFAWDIYQRAQLAQCLGHRGEDGLGLAGAWKLQ
                     YQDRKGQYYFRIPDNLIKKHVAPFCDIPTLAYWVEAIVASGGRIENPEVALEFLFSRQ
                     LPNGAFVDAYQAEDVLESNAHVVATAQAVAVIRSLGQEVPRREQCVAWIQSCQDESGG
                     FRWHPSYRYPGNKPDVWYTWAAVSALDRLGAKPMDIKGCLRWLNSLQNPDGGFGDRPG
                     WNSRLYSTYYAVHALDILNASGGGARAAITAKKATAARRIIPEGKYQIYQASLKTPPG
                     GPEMIETARKLRLNFLGVKDNSPNTPPVPISVAWEHIKKQGYPMEVVSIPEQYGHQLK
                     WIGGHPANHVANFFLPLGDSPEIKEFLAAVDTAGRAGLPWPEYVQKVIQPVVQRGSLF
                     YPELDYEMMNAYIVYDGGLEDGLGYNAVVGGLGWPIWDWIRMFPYRERWVGKLPIIVD
                     ADAHGEIAKWLEPLDRQRLLYLAEHYDLPHFLDACRQVRTVCVIRGGENKDELAFYGD
                     PAVVEYVKRHQSEWQWW"
     assembly_gap    7575..7674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7675..9060
                     /locus_tag="PLD05_09865"
     CDS             <7675..9060
                     /locus_tag="PLD05_09865"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HOP77788.1"
                     /translation="IGGCWWGVNMQDATAAESAWVLRAAVPLAEKTWRWNWASWDQEK
                     IVTFGEYQYTVFWDADGVFVLARRHLADNTVQLVRMPGWRLSVDDAHRNTCLGISEKD
                     GRLHFSWDHHNNPLNYGRTRAGFLTQPPAQISAGDFEPRQPPAEGDFPMSRVTYPRFI
                     NDNKGGLYLFYREGGSGNGDNYLWRYDAETGRWTMVGCVFSKRGTYAPWNNSTSRNAY
                     FHDLLFDRNNRLHATWVYREVAETWASNHDLHYAYSDDGGATWFNNSGEKIADMSADD
                     PIELNDAGIVVREIPVFSWLMNAGCMALDSKGRPHVMTFKLPEPREPNQLKHDPPDVI
                     RHDLHFVHYWRNEDGTWHGGEPIRPINGDIPGESVARGDIVFGPDDTLFFYWVDRNEG
                     RFRYLVASAADQWQAWQVDWLLDKGFSGGDASKHDRRRWQNEGVLSFTARFGDQGFGI
                     VDLKMSQGAAP"
     gene            complement(9081..>10570)
                     /locus_tag="PLD05_09870"
     CDS             complement(9081..>10570)
                     /locus_tag="PLD05_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77789.1"
                     /translation="GRLMLCMHGQMFDFPRGFSAQACGGIRPVCTHLRYIPDFCHWNG
                     RVVLGADDASMMHNPMCGQAQSNLWFGTPEDLCRFGPTTGWGGVWMDDPVTADVPSDP
                     FLIAGYKQRVVHIGQRGDRAVTFSFEVDRTGNGQWEKLFDVEVQPGTYRWSVIPEDVA
                     AEWVRVRASADCRATVYFHCWSPRPAKADETTLFAGLADLSSSVEHYGGLIRPAAHNR
                     NLQWLAWKGNAKGEIQRLGYHEVDITREGRPILVTPKEDRTEEVEKIASLKADFEVDT
                     ASVIVRDTSGQRWRLPRGPAAFDQWVGQMRAVREVASERFLANIHGTFYEIPRDAQNQ
                     PDWSKMKPVCSHSKRIIDFCSWRGLLVLAGVADNAAEDGQVFGNPGGPRLWFGCLDDL
                     WKLGPPRGKGGPWNNTAVRPGEPSDPYLMTGYARKTLVLSHDADEPVRFTVEIDFDHT
                     GFWRYAEFEVLPGKPFRHDFGDFHAHWIRFRCDKPCRATTWLEYE"
     assembly_gap    10571..10670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10671..11374)
                     /locus_tag="PLD05_09875"
     CDS             complement(<10671..11374)
                     /locus_tag="PLD05_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP77790.1"
                     /translation="MQQLRMFVLGMFLATASAGSLAQEAVNVPATEEQRQYVCISGIY
                     PHLAVFNQPEDPSDRPQHGECGIGAIAVWADRLWYLTYPQHKTMGSNDKLYELDADMN
                     VVIRPESVGGTHAARMIHRESEQLILGPYFIDKQRNVRAANLKELRGRMTAIMRHLDD
                     PANKVYFFDMEGAIYEVDVHTLAVNRLFVKPVPGWHGKGGYTAQGRVVIANNGEVGPA
                     DGYRHVLVGGPAQGEEA"
     gene            complement(11447..>11842)
                     /locus_tag="PLD05_09880"
     CDS             complement(11447..>11842)
                     /locus_tag="PLD05_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77791.1"
                     /translation="EGKTDKIREVLYGVYCGGAKPGIRCVKKGDWKLIKYDAPELGVH
                     VTQLFNLAENPHEFLIEHHNPNVTALTGVVPEPHQVSLADDPRYAAKRAEMEALLLAE
                     MRRLDDPYRLWDQPDDGLRMPQRQSLSKE"
     assembly_gap    11843..11942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11943..12786)
                     /locus_tag="PLD05_09885"
     CDS             complement(<11943..12786)
                     /locus_tag="PLD05_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77792.1"
                     /translation="MHFSIKRGFGRRVGTIGAILLGLLLFECCGLSCLAAERRPNFLF
                     IIVDDQSPNDLKIYNPRSILNTPNIERLAAEGVVFDGAYIMGSWVGAVCLPSRTMIMM
                     GRTVWHIPAPGKNPANPHTNNPKLVPPDLPDHTLPAVFNRAGYATMRTCKIGNSYEAA
                     NKLFTVRRDATKRGPTDETGSAWHAEQVLEYLAERESRGDKAPFLIYFGFSHPHDPRD
                     GKPELLARYGAVNHTDRNTLPPAHPNQPLLPVNYLPAHPFDTTHMDVRDEVAVSGVWD
                     RRDER"
     gene            complement(12979..13842)
                     /gene="ilvE"
                     /locus_tag="PLD05_09890"
     CDS             complement(12979..13842)
                     /gene="ilvE"
                     /locus_tag="PLD05_09890"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOP77793.1"
                     /translation="MSLKVYINGKYYDKEEAKISVYDHGLLYGDGVFEGIRSYGGKVF
                     RLREHLDRLWDSAKAIWLTIPMTKEEMAAAIEETLRINGIEDGYIRVVVTRGVGTLGL
                     DPNRCHQPQVIIITDHISLYPKEYYENGLEIITVSTIRNHPAAVSPRIKSLNYLNNIL
                     AKIEGLQAGCIEALMLNHKGEVAECTGDNIFIVRRGELLTPPREAGILEGITRAAVIE
                     LAQQANIRVLEVPLTKHDIYIADECFLTGTAAEIVPVVKVDSRPIGDGKPGPITRDLM
                     ERFHRLTRNHC"
     gene            complement(14391..>14737)
                     /locus_tag="PLD05_09895"
     CDS             complement(14391..>14737)
                     /locus_tag="PLD05_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOP77794.1"
                     /translation="ESRFRLTSRRDRPLISTVTEGELLALAYSRQWSPKKLEALRKML
                     ENFVRVDAGLAPVVEWYAKLDAEARRRGAPRGQNDLWIAATAKATGAVLLTCDKDFDW
                     LDPDYLKVFRMP"
BASE COUNT         3153 a         4004 c         4171 g         3109 t
ORIGIN      
        1 gagaaaaccg gcagcgattc gagccaccct cacccccgac ccctctcccg ctgaatggga
       61 gaggggagat tgggcaatac gcttttagcc gaaaaaggaa gatgatcatg acctgcacac
      121 ggcgttcctt cttgcaacaa ggcgcggcgc tactggggta tcttgccgct ggtcttgaga
      181 atcggttttc ccgtggtagc ctgagcttgg aggcgtcgga aacggtgccg gagctgacgt
      241 cgatcccgat tgtcgatacg catcagcatt tgtgggactt gtcaaaaatt cgccttccct
      301 ggttggccgg tgacgagcga ctcaatcgga actatcttgt ggaagactat ctggaagccg
      361 tgaagggctt gccggtggtc caggccgtct atatggaagt ggccgtggcc gatgaggacc
      421 ttgtcaaaga agctgaatgg atcattgaga tttgccgtag tgggcgggga ccgacggtgg
      481 ctgccgtcat tggagggcgt cccgccagcg acgatttccg gacctatctt gctcgctttc
      541 gcaataaccc ggtggtcaaa ggtgttcgct ggattccacc caatagtaag ttgggacaca
      601 agctttattt cggcccacag atgcgggaca acttgcgact attgggtgaa tggggaatga
      661 gtttcgacat ctgcatcccg cccgactggc tgagcgacgc ggcagagttg gtagatgcct
      721 gtcctgacac acggtttatt atcgatcatt gtggcaacgc tgacccccag caatttggtc
      781 gctggggaaa ggagcgcggc gaagaggcca ctcggtatgt agagcagtgg cggcgggcaa
      841 tggacggcct ggcagcgcga aaaaatgtcg tctgcaaaat ctcggggatc atcgcgcgcg
      901 taagaccaga cgattggcgt cccgacgatc ttgccccgat cgtcaaccac tgtttggagg
      961 ccttcggctc tgaccgtgtc atgttcgccg gagattggcc agtgtgcaca aaaggtgcgc
     1021 ctctccgggc gtggattgcc gccttacgtg agatcacggc aagccgatcc ctggaagaac
     1081 gccgaaaact cttccacgat aacgccgtga gattctatgg gttagagcct atcccaaagt
     1141 tcccacgccc tctaggagga ggttagggtg agggcaaggg actgtcggag tcgatcgaga
     1201 aacccggtag cgattcaagc caccctcacc cccggcccct ctcccgccga acgggcaagg
     1261 ggtggttgtg ggataagctc gtagctcgca ctggatgatt tggcttcatg cggtcaggcg
     1321 aacatccgag gccggcagga gccacatgat tgcgtcgtcc taggaagtgc ccttgggagg
     1381 ggtcaaacat gggtctgcct gattaagcct taggtgcgag catcgcgcga gagcggaacc
     1441 agccgatggt gacacgctca tgacaaacaa gcggtacgcc aggagggagg ttccctttaa
     1501 ggcgatcaaa cgcgctccgc gggggataac gagacagggg ccgcaatccg tcgtggccga
     1561 gggcaaggca aacacccgcc gagtgaaaat acggcggacg ccggggacgg cgtccctccc
     1621 aaacggccga agttaatcta aacgctccct ggagaaatga cggacgccgg ggacggcgtc
     1681 cctcccaagc tgaaggcccg cccgaggtag tgcgcaaaat ggcgcccgga gtgttgcgcc
     1741 acatttgcag gaacgtgagc cctggcgtgc gatatatcgg cgattgatgc accatcgtgt
     1801 ttcacggcca ggacattcac gcctacgaca gcatgtcgcc ccgcatgaat agctcacgga
     1861 tcgttaaagg taagccaatg cttgccctcg ttgtttaccc agtggggttt tcgatccacc
     1921 gcgcatagca ccgagcgcag agatagaaac tcaagcggcg atagacctga tccatggcct
     1981 cctgggccga aaggtgggat agctcttcca acacgtcgcg aaaagccgca ttgagatcgt
     2041 tggggtaatc ctcgaaatcc aattggggcg gtgacggatc ggccatggcc tcgatcttga
     2101 cgagataaca acttccccga ccgagctgaa gctccacacc acaccgctga caagccaccg
     2161 gataatgaaa gcgaggatcg tcattctcca tgtcccccat tttagcttaa gaccacttat
     2221 cgggctacga tgatgcggca actctttctc cctgaggatg cccatttgcc cgtccgtagg
     2281 cggctcctag aggtcgcagg aattgactct gtaccgatcc gtattaaatt gaaggggcag
     2341 agcgtggaca gtttcctggc gtggcatcgc atgtcgtagt gtgctgcgga aactgtttat
     2401 ccgctggcga cgtgtttcga tggtcataca gtatttcttt tcgtggggag gtttcagcca
     2461 tgaactcggt cacacgttgg cttccgcgcg ttggcttggc cgcggccttg ggactgttat
     2521 cgggtatgtc gttacttgca tctgaaaccg cgcagcggcc aaatgtgctg attatcacaa
     2581 ccgatcagca acgtgttgac gcggcaagcg tggtgggaaa caaatggctc aagacgcctc
     2641 atctagatgc cctcgcagct caaggtgttt acttcacaaa gtcttattgt tcgcatccgt
     2701 tgtgtagtcc gtcgcgggcg agccttcacg cagggcgatt tccccacgaa attggcgtgg
     2761 accacaacaa tatgtcaata actccgtccg tcacgatttc cggtcaggtt tttcaagagg
     2821 cgggctacga cacaggttac gccggtaagt ggcatatacc gacggtttac ccgaccgatg
     2881 gcattccggg ttttaccgtg ctgaataata ccacgcggca gggaaaacta gcgcatgacg
     2941 tggatgacgc gacaatgcag gttgccatcg acttcatcaa gcagaaacgg gataaaccgt
     3001 tttatctggt tgtttcgttt atcaatcctc atgacatttg tctgctggcg ggcgaggata
     3061 gccccctcct tgagcaggtt tggcggaagt acggtccccc ggcgggagcc gagcttccac
     3121 cgcttcccgc aagttttacc ctcccagaaa atgaaccagc gtggttgctc cagcgacgtc
     3181 ccaaacatcc tcaatgggat gaaacgcact ggcggcgata tatttacgcc tattatcgta
     3241 tggtcgagga tgttgatcag cagatcggag ccgtcctaaa ggcactgaag gaaactgggc
     3301 aagaagagaa cacgctggtc atcttcacaa gcgaccacgg ggagggcttg ggttcccgtc
     3361 gctggacggg caagatgatg ttctttgacc cagaagcggc tgtgcccctc atcgtcagtt
     3421 ggaaggggaa gacccccgcg ggccggatcg acaaggagca tttggtctcc accgtcgatg
     3481 tccttccaac gatctgtgat tatgcgggaa tcaaaccgcc ggagaacgtc cgtggacaaa
     3541 gcctccgacc aatcattgag aatcctgccg caccatggcg gtcgtttatt gtctcggaga
     3601 ttgcccacac accggcgttg ggacggagct tcatggtgcg aacggaccgc tacaaatatt
     3661 ttgtgatccc tgttgggcag gagaagccgt tggagatgct cttcgacatg gaaacagatc
     3721 ctcacgaatt gaagaacttg gcttccgatc cgaaccactc cgagacgctc aaagagcacc
     3781 gcgccttgct cgaccaatgg aagcaaacca cagaagaagc gaagtatccc gtcctaccca
     3841 acccccaagc ggcacgcccc agggcacaac gagccaagca ggctgcacca taagagggtg
     3901 tttgtcaccg tcaagcgata cgggcacgca acggccacgt tgtgcggcgc atggcgttgg
     3961 acgagctgat ttggtaacgt gaagccatct taacgatgct tggcttagcg aatctgttca
     4021 tgttaggagt caaaatcatg aagccgttgt tccgcggtca acaagggcca actgtgtttt
     4081 gggcgtcaat gttggttgca attggttgtt cttatcaatg ggggcaagcg ggatgggcta
     4141 cggagaaacg gccaaacatc ctccttatcg aggtggatag catggatggc cgagtcatgg
     4201 gatgcatggg ttatcccgcc atggtgcggg caacgccaaa cctcgatgcc ttggccaaac
     4261 ggggggttct tttccgcaat gcgtacacca acaaccccat ttgctgtcct tcgcgggcca
     4321 gcatgtggag tggtaaatac acgtttcgca tcgaggcctg gaataattac aaggggcttg
     4381 aacccgggac gccgacgttc cgcgacgaat tggaaaaggc gggctatctg acacaaacct
     4441 tcgggaaatt ggattacctg tccggcgccc attcggtgcg ggccagggtg acggcgtggc
     4501 ttcgctcggc caacatcgaa cggccacagt atcgcatcgg gccgccgcgg gtcattgagg
     4561 aagacctcgt gcgggttcac gccggagact ggcgaaatgc ggatcgcgcg gtggcctggc
     4621 taaaggacaa cgcaaaaggc gataagccct tttggttgta cttaggcttg agtttgcctc
     4681 accctgcctt tcaaacgtct cgttattatc tgcggatgat tgatgaggcg ggagtcgatg
     4741 tgcctcccct cgatcaatat gaccattccg tcatgcgcta ccagcggtta gtcaaaaact
     4801 gggagcatgg ttactccgac gagatgatca agctcgtccg gagggtctat ttcgccatga
     4861 tcgccgagac tgacgccatt gtcgggcacg tgctgcaagc cctccgtgac tgcggtctgg
     4921 aagactcgac ctacgtgatc ttcatcagtg atcatggcga gaacgcgatg gaacaccgac
     4981 tctggtacaa aatgaatcac tatgagtcgt ccgctcgtgt ccccatgatt atcgccggac
     5041 ccggtgtgag acaaggcgtc gtggaagaaa agctggtttc gctgattgac atttacccga
     5101 cgttaatgga catggccaat tggcccaagc cgcaggacct ggatgggcat tccctcatgc
     5161 cggagctatt gggccagccc agctcccggc cggactgggt attgagcgaa taccacgaca
     5221 ccgcatgcaa cacaggcagc ttcatgatcc gccggggtcc ttggaagtac atcgcctacc
     5281 ctggttatga accgatgctt ttcaacctcg atgaagaccc ccatgagatt cgcaatctcg
     5341 caccgcaatt gcctgacaag gtgcgggaga tggaccaact gctgcgttcc atcgtggatt
     5401 acgaggctgt cgatgccaag gccaaggcat acgaccgggc aagttttgcc gcatggcggg
     5461 aggaacataa gaagaaaggt gactacgaag agctaatggc catcatattc agcggttacg
     5521 acggtgtgaa cgccgcgaaa gccaagccct ggacgcccga ggatgaggcc aaagtcgaac
     5581 gctggctcaa aggcgaacaa tgatcacaag tgacgagagc gtgtgggaat ctcatcatga
     5641 ctaacaaaac gaatggatca gccgccacgg tgtggcgatt tgtgccgtgg cgcttgctct
     5701 tttggatagc tctttgcagt gtggggcctc tctgcctgtc tggacagatc ggtttcggtg
     5761 ccgaacaagg ttccgcgggt gacccggtgg aaatcatcga ggcgatggtt gatgcccaag
     5821 caaccatccg ctatttgctc gcgcatcaaa gggaaaacgg tgcctttggg ccgatgggac
     5881 atcagcacac cgacctggca tggaattacc ccgccgcgcg tgctttgatc ctgctggagg
     5941 ttgaaatccc gcgggtggag gattgtttcc gcaatggaca ggatgccctt tacaaacacc
     6001 caggctcaca taatacccaa tttgcctggg atatctatca gcgtgcgcaa ctggcacaat
     6061 gcttgggtca tcgcggggaa gacggactgg gcctggccgg ggcgtggaag cttcaatacc
     6121 aggaccgaaa gggccagtat tactttcgga ttccggataa cctcatcaag aaacacgtgg
     6181 cgccattctg cgatattccc acgctggctt actgggtcga ggccattgtg gcctctggcg
     6241 gccggattga gaacccggaa gtggctttgg aatttctctt ttcgcgacaa ctcccaaacg
     6301 gggcatttgt ggacgcttat caggcggagg acgtgttgga aagcaatgcc cacgtcgtag
     6361 caacggccca ggccgtggcg gtaattcgat cgcttggcca ggaggttccg cggcgggagc
     6421 aatgcgtggc atggattcaa tcgtgccaag acgaaagcgg tggtttccgc tggcatccca
     6481 gctatcgtta tcccggcaat aaacccgacg tgtggtacac ctgggcggcc gtttctgcct
     6541 tggaccgcct cggtgccaag ccgatggata tcaaggggtg tttgcgctgg ctcaacagtt
     6601 tgcaaaaccc ggatggcggc ttcggggatc gaccgggctg gaactcgcgt ttgtatagca
     6661 catattacgc ggtccatgcc ttggatattc tcaatgcttc gggtgggggc gcgcgggcgg
     6721 cgataacggc caaaaaggca accgccgcgc ggaggattat tcctgagggc aaatatcaaa
     6781 tttatcaagc ctcgctaaag actccgccgg gtgggccgga aatgattgaa acggccagaa
     6841 aattgcggct caacttcttg ggcgtcaagg ataatagccc aaacaccccg cccgtgccta
     6901 tttctgtggc ctgggagcac atcaagaaac aaggttatcc catggaagtc gtttccattc
     6961 cggagcagta cggccatcaa ttgaagtgga ttggaggcca tccggccaac cacgtggcga
     7021 atttcttcct gccactgggc gattcacctg agatcaagga gtttttggcg gctgttgata
     7081 ctgcgggtcg ggcgggcctt ccctggcctg agtacgttca gaaggtgatt cagcctgtcg
     7141 tccaacgggg ctcgctcttc tatcccgaat tggattacga aatgatgaac gcctatatcg
     7201 tctatgacgg tggtttggaa gacggtttag gatataacgc ggtggtgggt gggcttggtt
     7261 ggccgatctg ggattggatt cgcatgtttc cctaccgtga gcggtgggtg ggcaaactgc
     7321 cgatcattgt cgatgccgac gcccatggcg agatcgcaaa gtggctggaa ccgcttgacc
     7381 gccagcgtct gctgtatttg gcggagcatt atgaccttcc gcattttctg gatgcttgcc
     7441 gtcaggtacg aacggtgtgc gtcatccggg gcggcgagaa caaggatgag ctcgcctttt
     7501 acggagaccc ggccgtggtc gaatatgtca aacgccacca aagcgagtgg cagtggtggt
     7561 agcgcccgcc tgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcggg
     7681 ggctgctggt ggggagtaaa catgcaggat gccacggctg ccgagtcggc atgggttctt
     7741 cgtgctgcgg ttcctcttgc cgagaagaca tggcgctgga attgggcgag ctgggaccag
     7801 gagaagattg tcacgttcgg agagtaccaa tatacggtgt tttgggatgc ggacggcgtt
     7861 ttcgtcctcg ccagacgcca cctcgcagac aataccgtac aacttgtccg aatgccaggg
     7921 tggaggctct cggtagacga tgcccaccgc aatacctgcc tgggcatttc cgaaaaggac
     7981 ggtcgtttgc acttttcctg ggaccatcac aataatcccc tcaattacgg gcgaacccgc
     8041 gcgggctttc tcacgcagcc gccggcgcag atctcagccg gcgacttcga accacgccag
     8101 cccccggccg aaggcgactt tcccatgtct cgtgtgacct atccccgctt catcaacgat
     8161 aacaagggag gcctttacct gttttatcgc gaaggcggtt ccggcaatgg ggataattac
     8221 ttgtggcgat acgacgccga aacgggacgc tggacaatgg tgggatgtgt ctttagcaaa
     8281 cggggcacct acgcaccctg gaacaacagc acctcccgca atgcctattt tcacgatctt
     8341 ctcttcgacc gcaacaatcg acttcatgcc acttgggtat atcgggaagt tgccgaaacc
     8401 tgggccagca atcacgacct gcactacgcg tacagtgatg atggtggggc cacatggttc
     8461 aacaattcag gcgagaaaat cgccgatatg tccgccgatg atcccatcga attgaatgac
     8521 gcgggcatcg tcgttcgaga aattcccgtg ttttcgtggc ttatgaatgc cggttgtatg
     8581 gcgctggatt ccaaagggcg gcctcatgtc atgacattca agcttcccga gccgcgggag
     8641 ccaaaccagc tcaagcacga tcctccggat gtcatccggc acgacctgca ctttgttcat
     8701 tattggcgca atgaggacgg aacatggcac ggaggagaac cgatcagacc gattaacgga
     8761 gacatccccg gcgaaagcgt ggcccgcggc gacatcgtgt ttggcccgga cgatacactt
     8821 tttttctatt gggtcgatcg aaacgagggg cgatttcgct acctggtggc ttccgccgcc
     8881 gatcagtggc aagcgtggca agttgattgg cttctcgaca aaggcttcag cggtggtgat
     8941 gcttccaaac atgaccgccg ccgttggcaa aacgaaggtg tgctttcttt taccgcacga
     9001 ttcggcgacc aaggttttgg catcgtggac ctgaagatgt cgcagggagc cgccccctga
     9061 gcggatcacc tcatagcggc ttactcgtac tccagccaag tcgttgcgcg acacggcttg
     9121 tcgcaccgaa aacgaatcca atgggcgtgg aagtcgccga aatcgtgacg gaacggcttg
     9181 ccggggagaa cctcaaactc tgcgtaccgc caaaagcctg tatggtcgaa atcgatctcc
     9241 accgtgaaac ggaccggttc atcggcgtcg tgcgacaaca caagtgtctt gcgggcataa
     9301 ccggtcatga ggtaaggatc ggatggttcg ccgggacgca cggccgtgtt attccacggg
     9361 ccgccttttc ctctcggtgg accaagtttc cacagatcgt ccaaacaccc aaaccataag
     9421 cgggggccgc ccggatttcc aaacacttgc ccatcttcag cggcgttgtc cgcaacaccc
     9481 gccaggacga gaagtccgcg ccatgagcag aagtcgatga tccgcttgga atgactacag
     9541 acaggtttca ttttggacca atccggctgg ttctgagcat ctcgaggaat ctcgtaaaac
     9601 gtcccgtgga tgttggccag gaatcgttcg gaggccacct cgcggactgc ccgcatttgt
     9661 cccacccatt gatcgaaggc ggccggtccg cgcgggagcc gccaccgctg tccggaggtg
     9721 tcgcgaacaa tgacggaggc cgtatcgacc tcgaagtcgg ctttcagcga cgcaatcttt
     9781 tccacttcct ccgttcggtc ctccttagga gtgacaagga tgggtcggcc ttcgcgggta
     9841 atgtccacct cgtgatagcc caggcgctgg atttcacctt tggcgttccc cttccaggcg
     9901 agccactgaa ggttccgatt gtgggcggcg ggccggatta agcctccgta gtgttccacc
     9961 gatgacgaaa gatcggccaa tccggcaaac agcgtagttt cgtcggcctt ggcgggccgg
    10021 ggggaccaac aatggaaata cacagtggcc cgacaatcgg cggaggcccg tacccgcacc
    10081 cattccgccg ccacatcctc cgggattacg ctccagcgat acgtccccgg ctgtacttcg
    10141 acgtcaaaga gtttttccca ctggccattg cctgtgcggt ccacctcgaa agagaaagtg
    10201 acggcccgat ctccccgctg tccgatatgt accacccgtt gcttataacc ggcgatgagg
    10261 aaaggatccg acggcacgtc ggcagtgacg ggatcatcca tccatacacc gccccaaccc
    10321 gtcgttgggc caaaccggca taaatcctcg ggcgttccaa accacaggtt ggattgtgcc
    10381 tggccacaca tcggattgtg catcatcgaa gcgtcgtcgg ctccgaggac gacgcggccg
    10441 ttccagtggc agaaatcggg gatgtatcgc aggtgcgtac agacgggacg aatgccgcca
    10501 cacgcctggg cggaaaaccc tcggggaaaa tcaaacatct gcccgtgcat acaaagcatc
    10561 aatcggcccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcttcttcgc
    10681 cttgggcagg gccacccacg aggacgtgac gataaccatc agcgggccca acctctccgt
    10741 tgttggcgat gaccacacgc ccttgggccg tatagccgcc tttgccgtgc caccctggaa
    10801 cgggcttcac gaaaagacga ttcaccgcca gcgtgtgaac atcaacctca taaatggcac
    10861 cttccatgtc aaagaaatag accttgttag cgggatcatc caggtggcgc atgatcgccg
    10921 tcatacgccc acggagttcc ttgaggtttg ccgcccggac attccgttgc ttgtcgataa
    10981 aatatggtcc taggatcagt tgctccgatt cgcggtggat catccgggcc gcatgggtgc
    11041 cgccgacgct ttccggtcgg atgaccacat tcatgtcggc atccaactcg tataacttgt
    11101 cgttactgcc catcgtcttg tgttggggat aggtgagata ccacagacgg tcggcccaaa
    11161 cagcgatggc tccgatgcca cattcaccgt gctgcggccg gtcggagggg tcttctggct
    11221 gattgaacac ggccaaatgc ggataaatgc cactaatgca cacatactgc cgctgttcct
    11281 cagttgccgg gacattgacc gcctcttgtg cgagcgaccc cgccgaggca gtagccagga
    11341 acatgcccaa cacgaacatt ctcagttgtt gcatagatgg gtcctccaat gcttgtgatt
    11401 atcgtgcgat tgtgaaatca tgtgggtcgt ggcagcctcg tgcgcatcac tccttcgaca
    11461 gcgactggcg ttggggcatc cgtaagccat cgtcgggctg gtcccacaac cggtacggat
    11521 catcaagacg ccgcatttcg gccagaagga gggcttccat ctctgcgcgc ttggccgcat
    11581 agcggggatc atcggccaga ctcacctgat ggggctccgg gacaacgccg gttaaggccg
    11641 tgacgttcgg attgtgatgt tcaatcaaaa actcgtgagg attctcggcc aaattgaaaa
    11701 gttgcgtgac gtggactccc aattcggggg cgtcatattt gatgagtttc caatcccctt
    11761 ttttcacgca gcgaatgccc ggctttgccc cgccgcaata tacgccataa agcacttcgc
    11821 ggatcttgtc cgtcttgcct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnttctctcg tcgcggcgat cccaaacacc actgacggca acctcgtccc gaacatccat
    12001 gtgtgtggtg tcaaacgggt gggccggcag gtaattaacc ggcaataggg gctgattggg
    12061 atgggcgggc ggtaatgtgt tgcgatcagt atggttaacg gcgccgtagc gggcaaggag
    12121 ttccggcttt ccatcgcgtg ggtcatgcgg atgcgaaaat ccaaaataga tgagaaacgg
    12181 ggccttatcg ccacgagatt cccgttcggc cagatattcg aggacctgct cggcgtgcca
    12241 ggcactgccc gtttcgtcgg tgggaccccg ctttgttgcg tcccgcctta cggtaaacag
    12301 tttattcgct gcctcgtagc tgttgccgat cttacaggtc cgcatcgtag cgtagcccgc
    12361 tcggttgaac acggccggca aggtgtggtc cggaaggtcc ggcgggacga gcttagggtt
    12421 atttgtatga ggattggcgg gattcttccc cggcgccgga atatgccaca ctgtccggcc
    12481 catcatgatc atcgttcgcg atggcaaaca aacggctccg acccacgagc ccataatgta
    12541 ggcgccgtcg aagaccaccc cttccgcggc cagtcgttca atgttcggcg tgttgaggat
    12601 cgatcggggg ttatagattt tcaggtcatt gggcgattgg tcatcgacaa tgatgaataa
    12661 aaaattggga cggcgttcgg ctgcgaggca tgatagtccg caacattcaa ataggagaag
    12721 cccgagcagg attgcaccga ttgtcccaac gcgcctgcca aaaccgcgct tgatgctgaa
    12781 atgcatggaa aaccacctcg ccggacaaca cgtgactgcc aagacaccca aaccatcgcc
    12841 cgatccggct gataatcttt cagccagctg cccagtcata acagcggtta tgccagtggc
    12901 aaaacgtttt cgacaaagtc attgctaaat ggaacggttg caaacccgtt tgccccgtcg
    12961 tgccccgctg tcagggaatt aacaatgatt cctcgtcagt cggtggaatc gttccatcaa
    13021 gtcacgagtg atcggaccgg gctttccatc gccaatgggc cgactatcga ccttgaccac
    13081 gggcacgatt tccgccgctg ttcctgtcag gaaacattca tcggcaatgt aaatatcgtg
    13141 ttttgtcagg gggacttcaa ggacacggat attggcttgt tgggctagct caatgacggc
    13201 tgcccgtgtg atcccctcta gaattcccgc ctcgcgcggc ggtgtcagca attcaccacg
    13261 ccggacgatg aagatattat cgcccgtgca ctcggccacc tcacccttgt ggttgagcat
    13321 caaggcctcg atgcagcccg cctgaagccc ttcaatcttg gccaagatgt tattgaggta
    13381 attgagggat ttgatccgtg ggctgacggc ggccgggtgg ttgcggatcg tgctaaccgt
    13441 gataatctcc aagccgttct cgtagtattc cttgggatac aaagagatgt gatccgtgat
    13501 gatgattacc tgcggctgat ggcagcgatt aggatcgagg cccagggtcc caacgccgcg
    13561 ggtcaccacc acgcgaatgt agccgtcctc gattccgttg atacgaaggg tctcttcgat
    13621 ggccgctgcc atctcctctt ttgtcatggg aatcgttaac caaatggcct tggcggagtc
    13681 ccacagccga tcgaggtgct cgcgaagccg aaagaccttt cccccgtagc tgcggattcc
    13741 ttcaaagacg ccatccccgt aaagtaggcc gtgatcgtaa acgctgatct tggcctcctc
    13801 tttgtcgtaa tactttccgt tgatgtaaac cttcagcgac atggcgtgat cttagacctc
    13861 tagttttgcc aagaaattaa gccgttggaa cgttatgcag acaaactatt agcgtaccat
    13921 gccagagatc gcttggcgag acgccggggg cacttttagc ggtaagatgt ccgcatgaac
    13981 gtagcactac ggtggagttg aaataaagcg atccgccgtg ccttgcacta cggagctatt
    14041 cagaacttac cgcataacct cccctctccc gttcggcgaa gaggggccgg gggtgagggt
    14101 ggctggaatc gctagcgctt tctcgatcga ccccgacgat cgcttgccct caccccaacc
    14161 ctctcccaga aggagaggga actttgcgat agtctctagg cggatttcgg cattgccaga
    14221 cagttcggcc tatcaaacat tgcagtttgc acctccatat tgcgaggcgt gaaaagggag
    14281 cgcgactttg ctgtacaagc taactctttt gccgccggac gctttgcaac acccctcaag
    14341 cagcaccttg gacggggcct ccggccggcg aattgcaaga gttcagctat tcagggcatc
    14401 ctgaagacct ttaagtagtc tggatcaagc cagtcaaaat ccttatcgca tgtcaaaagc
    14461 acggctccag tcgccttggc tgtggcagca atccaaaggt cattttgtcc ccgcggggct
    14521 cctctgcgtc ttgcttctgc gtcaagcttt gcgtaccact caaccaccgg tgccagcccc
    14581 gcatccacac gaacgaaatt ttcgagcatt ttgcgcagcg cttcaagctt cttcgggctc
    14641 cactgcctgg agtaagccaa agccagcaac tcgccttctg taacggtaga gatgagcggc
    14701 cggtctcgcc gcgatgtaag tctgaaacga gactcga
//
LOCUS       DAOSUT010000097        14685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2471_length_14685_cov_9.468489, whole genome shotgun sequence.
ACCESSION   DAOSUT010000097 DAOSUT010000000
VERSION     DAOSUT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_14685_cov_9.468489"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLD05_09900"
     CDS             <1..734
                     /locus_tag="PLD05_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77795.1"
                     /translation="AMRAFHEDWLHWHKWRDFATGNLGNWASHSANIAFMGLDIAKLW
                     DPQVNPNQRIRVTAKVQDRAVNAFPSWETVVWKIPPRGDWPALEIEWFNGAGAPGKRD
                     QVESLMGKKLDWGDAGRRKWDDHAGCLICGDKQLLHATGHNSSFTLLPSGRTSDEEGP
                     PQILPRSGSHEREWAAACKGGPRAMSHFGYSGPLTEFLMLGNVATLFPETELEFDPIE
                     TRIINHAEADAALRRVYRRGWELSS"
     gene            772..1947
                     /locus_tag="PLD05_09905"
     CDS             772..1947
                     /locus_tag="PLD05_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP77796.1"
                     /translation="MPRVFVVLACTLLLFTLTSLFANEEAWTLISSRNGDLEPPNGGD
                     QQTCLVVADFDNDGLLDFAVGERTKTPSVVWYQSTKQGWRRRVIEPSPLKPEAGGVAA
                     DVDKDGDVDLILGHDYSGNEICWWENPCPEFDKPWVRRAIKASGPRKHHDQTFGDFDG
                     DGRPELVSWNQGGKQLLMFPLPDHPKELKEWPSKTIFTWKQGQEYEGFPNTPVDIDGD
                     GTLDIVGGGCWFKYRGNGQFEPQVIDPTMRFTQCAAGQLIPGGFGEVVFSPGDADGEA
                     KWYQWTGKKWEAHVLGSVIHGHTCEIGDINLDGHLDILIGEMGSPGAGDRARIIVWYG
                     DGRGNFEQKVIRQGQGIHEGRLADLDGDGDLDVIVKPYHHNAPLVEILLNPIRSKGE"
     gene            complement(2123..2554)
                     /gene="rpsI"
                     /locus_tag="PLD05_09910"
     CDS             complement(2123..2554)
                     /gene="rpsI"
                     /locus_tag="PLD05_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOP77797.1"
                     /translation="MAKVVKKLLVPGKGTNDVVAVGRRKATAVARARVRPGTGIIAVN
                     GRPMEEYFPNLLYQNVVLQPLIMSGRRESVDVVVRVAGGGISGQADAVKLAIARALVK
                     MDPELHKPFKDAGLLTADARKKERKKYGLHGARRGTQFSKR"
     gene            complement(2621..3091)
                     /gene="rplM"
                     /locus_tag="PLD05_09915"
     CDS             complement(2621..3091)
                     /gene="rplM"
                     /locus_tag="PLD05_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOP77798.1"
                     /translation="MKKSYLAKPGEIKPKWYVVDATDKVVGRLASDIAMILMGKHRPT
                     YTPHVDTGDFVIVTNAEKVVFTGKKWEQKKYTWYTGYPGLRSETAAERREHKPEMILW
                     DAVRRMLPKNRLARKMIRKLKIYAGPDHPHQSQQPIPIELGVKGKGKFRRLITV"
     gene            complement(3355..4065)
                     /locus_tag="PLD05_09920"
     CDS             complement(3355..4065)
                     /locus_tag="PLD05_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOP77799.1"
                     /translation="MTPDSLNLGEYPAWIHHDIRPELLLISFPEKNGNVLEQDLLQKV
                     ASAAERHPWIRKVQSLHKVYPGTISASVVWRQPVLMVRIPEGLLPVDAEGVLLPSRDF
                     TPVEAAQYPRLVGLDVLPAGPAGQSWGDARVVQAAELAALLFEDWKKYGFRHIELIST
                     QITHPPKYEFVIVTHSGSRIVWGHAPSVVTTQEASADEKLARIEAYYRQHGSFDGIDG
                     LQEIDVRPLGGLRVRGLN"
     gene            complement(4468..5346)
                     /gene="murB"
                     /locus_tag="PLD05_09925"
     CDS             complement(4468..5346)
                     /gene="murB"
                     /locus_tag="PLD05_09925"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOP77800.1"
                     /translation="MPQWKGLERTIRANEPLAMHTWLGVGGPAEFYAEPRNADELADI
                     VRCAYEQGIPIRVLGSGSNILIRDEGVPGLVIQLSHPYFADVTIDGNIVSAGGGASLS
                     RVITTAVHEGLAGLEVLTAIPGTIGGALKGNVGAHGGDIGQWVKEVTVLDYTGQLSVK
                     DRTEVVFGYRRSSLDDLIILQAKFELEPEDPHELARRLQKLWIVRKAAQPMGHQASVR
                     VFRDPRGTTAAEVVEAAGLKGTRIGGAVVSERNANFIVTEPNCTTTDMLRLIDTIRTQ
                     VLNRLGVNLELDLEIW"
     gene            5613..6239
                     /gene="pgsA"
                     /locus_tag="PLD05_09930"
     CDS             5613..6239
                     /gene="pgsA"
                     /locus_tag="PLD05_09930"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOP77801.1"
                     /translation="MSKMIAGTSPRWFNLPNCLTLLRILLAVALFVTLSIHERVGYGV
                     SIGLFIVAAATDWLDGFLARRLNLVTALGRILDPFADKLIICGTFVFLAATPKMIETP
                     WGLHPWIVVVVISRELLITGLRSFLEGQTVDFSAKFSGKLKMGFQCITAVVAMMYLAD
                     TGYNTHLSYLLWLALVVSTYLTLLTTVYSGIAYIMIGWKKVRDLEAAG"
     gene            6250..7188
                     /locus_tag="PLD05_09935"
     CDS             6250..7188
                     /locus_tag="PLD05_09935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOP77802.1"
                     /translation="MASAVFGALVLVTLVWWAVVFTSRQTRQMLWPRYRFRQLPWRIR
                     DLLLVWLLFLLALAVSTVVSPLSTAKEGARVYQNEPQRHGLDSPGRDEDETTQGQHWV
                     LVLIQSDRSLKTVLLCLLGTGILGPIAEEILFRGFLQGWSHRVEWEHRRTRLNIAPIR
                     RQLPLGAASVALVAVLFATVHYRPDEPSPSVDVIRVILIRQTLAYSLFLPVALAIVAL
                     KDKPRGLRLIYDRRWFFCDITLGAGWFLAWIVPVYFVQGALKTILPWKAMADPCTMIV
                     IGTILGTLFARTGRLAASIVFHVLLNITSLSLAFLM"
     gene            7450..8553
                     /locus_tag="PLD05_09940"
     CDS             7450..8553
                     /locus_tag="PLD05_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77803.1"
                     /translation="MALGFGIVGCGLISKFHARAIGDVPDAKLVACFDVVKESAQRLA
                     QEFGCRAYDNLDAFLADPELNVVTIATPSGAHMEPAVAAARAGKHVIVEKPLEITLER
                     CDAIIKACEEAKVVLSTIFPSRFHQSSQWLKKAVDQQRFGRLTLGDAYVKWWRTQQYY
                     DSGAWRGTWQLDGGGALMNQAIHSVDLLAWLMGPVVEIQAATGLLAHERIEVEDVALA
                     ILRFSNGALGVLEATTAAYPGHLKRIEIHGNRGSAVMEEEDIIKWDFAEEHPDDQVIR
                     EKMASRISGGGGASDPAAIGHHGHARQFADVVRAIKEGTRPLIDGYEGRRSVEIILGV
                     YQAAELGRVVKLPLPGDPDLVARKVGVANLRKV"
     gene            8807..10135
                     /locus_tag="PLD05_09945"
     CDS             8807..10135
                     /locus_tag="PLD05_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOP77804.1"
                     /translation="MPIEFALPNLGENVTSGDVINVLVKEGDVIVGNQAVIELETDKA
                     VVEIPCPYGGQVKKVLVKKGDTVKVGQTILIVESEVAEKAPVVSPAKGPESQVAAAPP
                     EEKPSPPPAAPTPPPVAAVTAATTATAVAPEPSPLVEVDSRLLPAGPAARRLARELGV
                     NLATVQGTGRHGRITVEDVQRAAQSRAEAPRVTIPSPVVPPGEPGQDSWGPIRREKMS
                     KIRKTIAAQMTRSWTTIPRVTNFDDADVTDLENLRKSVPPDFLGEGLKLTFMPFVMRA
                     VSLALRHHPLLNASLDEENEQIIYKEYINLGIAVDTPRGLVVPVVRNADQMTIPQLAR
                     ALSEVARKARNVEFTVDELRGGTFTISNLGAVGGTYSTPIINYPEVAILLLGRSRWLP
                     VVRDGQIVARYMMPLSLSYDHRLVDGAAAARFLNEIIDWLQNPGKLLLVS"
     gene            10234..11652
                     /gene="lpdA"
                     /locus_tag="PLD05_09950"
     CDS             10234..11652
                     /gene="lpdA"
                     /locus_tag="PLD05_09950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOP77805.1"
                     /translation="MDIELVVIGGGPGGYAAAFLAADLGMKVAIVDQEPRLGGVCLLR
                     GCIPSKALLHVTRVIAETEELKEWGVDYSKPAVELNKLRARKEKVISILTGGLKQLAA
                     KRNVRVIHARAQFRDGQTLRLSPVNGGTLEDNVLRFQRCIIATGSRPVIPGLFPTGVD
                     RIMDSTVALDLPEIPDSLLVVGGGYIGLEMGMVYASLGSRVTVVEMMDGLLPGVDRDL
                     VRPLQKRLEGLFAAIYLNTKVAAVHLVDNGVEATFEGAKTGTERFSRVLVSVGRRPNS
                     DNLGLENTKVQVDSQGFIVVDARRRTAEPAVYAIGDVAGQPMLAHKAAHEGKTAVEAI
                     SGHKVAFEPRAIPAVVFTDPEIAWTGLTETDAKRKGIPYEVAVFPWAASGRAQAMGRT
                     DGFTKWIIDPKTERVMGCGIVGPSAGDLIGEATLAVEMAASARDLAETIHPHPTLCET
                     LGFAAEVFFGQATDLYRPRKKD"
     gene            complement(11802..12044)
                     /locus_tag="PLD05_09955"
     CDS             complement(11802..12044)
                     /locus_tag="PLD05_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77806.1"
                     /translation="MAYGYFIQGCPTCGRKVQIRLDYLGKYVTCHHCGAAFLAFGENR
                     PEKGDGNRPVPGHTIALKKTWQSEHMAVWPFGPEET"
     gene            12779..>14685
                     /locus_tag="PLD05_09960"
     CDS             12779..>14685
                     /locus_tag="PLD05_09960"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOP77807.1"
                     /translation="MLGQDNSRIWARYFAFLAISMAIIVVNIYLSSLFSPQPNKVQPP
                     PAEKVVADNASGEIKGTQPSSEPKTEDGPKQPSASEEETAVSQSTASRQEEKKEPDES
                     PRSLPKPPTRYLTLGSLDPAGPYRMLITLSNRGASVVSIEMSSLRYCDTEQPGGYLGA
                     IAIDPAAGEDGVRVDVVGPGTPAAEAGLLPGDVITEIENQRVYDLRSMEAILSRCRPN
                     QSVKIRVRRGEKEMVLDARLRRPPLRLIRREAGAPFSFEMALALANKTRLADLRAPFE
                     ERMAEVKDPMEKELLYLQWLHAEIPELGMRSENWHILADTSDPRRPETWGNTEIDSIP
                     QPRESVRFGIYHERLGLVVLKTYTLTKVPADKQTDPDNPSYHFDLTIDVINVGKDAQE
                     IAYELDGPNGLPTEGAWYARKMSNSWSAVGLRDVAVRMGKGAVQLVACPAIVKDKWGV
                     PWKDASETITALGVDAQYFSVIMLPKRSRLDEPWFDEARPIRVGPVEPDYQFLTNVSC
                     RVHSVPTVLQPGGQLTHEYTIFAGPKRQKLLATYGLHGLIQYGWFWFVADPMTKLLHL
                     FHNYLVFNYGLAIILLTVIVRLAMFPISRKQALNVQKMQELQPEIKKIQEKYKDLQER
                     NRATQELFRQH"
BASE COUNT         3282 a         3958 c         4056 g         3389 t
ORIGIN      
        1 ccgcaatgcg ggccttccac gaggattggc tgcattggca taaatggcgg gattttgcca
       61 cgggaaacct tgggaactgg gcttcgcact cagcaaatat agcctttatg ggattggata
      121 tcgccaagct atgggatccg caagtcaatc ctaaccagcg aatccgagtg acagccaaag
      181 tccaggatcg ggcggtgaac gccttcccca gttgggaaac tgtggtctgg aaaatccccc
      241 cacgcggtga ctggcccgct ctagagattg agtggttcaa tggggctggg gcccctggta
      301 aacgagacca ggtggagtcg ctcatgggaa agaaactcga ttggggagac gccggacgcc
      361 gaaaatggga cgatcacgcc ggttgtctca tttgcggcga caagcaactt ctgcacgcga
      421 cgggccataa ctcatcattt accctcctcc cttcgggaag aacctcggac gaagaggggc
      481 ctccccaaat cttgccccgg tcgggaagcc acgagcgaga atgggcggct gcttgtaaag
      541 gcgggccgcg tgccatgtcg cattttggat actcggggcc gctcactgag ttcttgatgt
      601 taggaaacgt tgccacatta ttcccagaga cggaactgga gtttgaccct atcgaaacgc
      661 ggatcatcaa tcacgcggaa gccgacgccg cgttgagaag ggtatatcgt cggggttggg
      721 aattgagttc ctgatgaatg taagtcacca attgctaagg ggagagttgc catgccacga
      781 gtgtttgtcg tattagcttg cacattgctc ctgtttacct tgacatctct ctttgcaaat
      841 gaggaagcgt ggacgctcat aagcagtcgg aacggtgatc tcgagcctcc taatgggggt
      901 gaccaacaaa catgcctggt tgtagccgat tttgacaacg acggtctgct cgattttgcc
      961 gtcggagagc gcacgaagac accgtctgtc gtctggtatc aatcgacgaa gcaaggctgg
     1021 cgccgccgtg ttattgagcc ttcaccactc aagccggagg ccggaggtgt ggcggccgat
     1081 gtcgataagg atggagacgt cgacctcatc ttgggtcacg attacagcgg caatgagatt
     1141 tgttggtggg aaaatccgtg ccccgaattt gataaacctt gggtacggcg cgctattaag
     1201 gcgtcgggtc cgcggaagca tcatgatcag acatttggcg atttcgacgg tgatggacgc
     1261 cccgaactgg tgagctggaa ccaaggtggc aagcaacttc ttatgttccc cctcccagat
     1321 catccaaagg aacttaaaga atggccatcc aagacgatct ttacctggaa gcaaggccag
     1381 gaatacgagg gttttcccaa cacgcctgtt gatatcgatg gtgacggaac tctggacatc
     1441 gtgggcggcg ggtgctggtt caagtaccgg gggaacggcc agtttgaacc ccaagtgatt
     1501 gacccgacca tgcggttcac ccagtgtgcc gcaggccaac tcattcctgg tggttttgga
     1561 gaagtggtct tttcgcctgg cgatgctgat ggggaggcaa agtggtatca atggacggga
     1621 aaaaagtggg aagcccacgt cctaggctcg gtcattcatg gtcacacctg tgagatcggc
     1681 gacatcaacc tggacggaca cctggacatc ctcattggag aaatgggatc accaggggcg
     1741 ggcgaccgag cacgaatcat cgtttggtat ggagatggtc ggggaaactt tgaacaaaag
     1801 gttattagac agggtcaggg aattcacgaa ggccgtcttg ccgaccttga cggggacggt
     1861 gatttggacg tgatcgtaaa gccttatcac cataatgccc cgctggttga gatcttgctc
     1921 aacccaattc ggagcaaggg ggaatagcac tggctgcctt gggagttcga acacctttct
     1981 gagaaaaacg aaatcggctg tgccaaccga agtcgacaca gccgattatc gaaggaggtg
     2041 ggctatgctt ctatttcgtt acgcaaccca tcgccaactg gttcgcggag ttgcaaaatt
     2101 tttgaggcaa tgattcggga ggttatcgct tggagaactg cgtcccacgg cgggcaccgt
     2161 gaaggccgta cttcttccgt tcctttttgc gggcatcggc tgtcaaaagc cctgcatctt
     2221 tgaacggctt gtgcagctct ggatccattt taaccagagc acgcgcgatt gccaacttca
     2281 cggcgtctgc ctgtccagaa atgccaccac cagccacgcg gacaaccaca tccacagact
     2341 cgcgtcgccc gctcataatt aacggctgga gaacaacgtt ttggtacagc aaattgggga
     2401 aatattcctc cattggccgc ccgttcacgg cgatgatgcc ggttcccggc cgaacccgtg
     2461 ccctagccac agccgttgcc ttccggcgtc ccacggcaac cacgtcgttt gtcccctttc
     2521 cggggacgag cagtttcttt acgacttttg ccataatagt tactaagctc gtccttttgg
     2581 tgatctttca aatcgtttca gaaatcaacc agaaaccttt tcacaccgtg atcaggcgtc
     2641 ggaacttccc cttacccttg actcccagct cgattgggat cggctgttgg ctttggtgcg
     2701 gatgatcagg acctgcgtaa atcttgagct ttctgatcat ctttctggcc aacctgtttt
     2761 tgggaagcat ccgccgaaca gcatcccaga gaatcatctc gggcttatgt tcgcgacgtt
     2821 cagcagcggt ctcagaccgc agaccaggat aacctgtgta ccaggtgtac tttttttgtt
     2881 cccacttttt gcccgtaaag acaaccttct cggcgttcgt cacaattaca aaatcaccgg
     2941 tgtccacgtg aggggtgtac gtgggacgat gtttgcccat caggatcatg gcaatatcgg
     3001 acgctaaacg accgactacc ttgtcggtgg cgtccacaac gtaccacttc ggcttgattt
     3061 caccgggttt ggccaaataa ctctttttca aagctagcct ctttctcatg aagtatcccc
     3121 gatgctacac tgatttggtc gaccatcggg cgatcaatcc ccgctgcctg accgcccaaa
     3181 acaaccctgc gatcaaggca gccttttctt gcgcgacatc aaagactcca atttatcccg
     3241 gattggattt ggcgacaagg gcgggggaac cgccaaaaag accagaaagc cgccaaccga
     3301 atgcgagatt ctcgtttccc ggcttttcgg acctcgccac tccaggaagc ggcgctaatt
     3361 caagccacga acgcgcaatc ctcccaaagg ccggacatcg atctcttgta ggccgtcgat
     3421 tccatcgaac gagccgtgtt gccggtagta cgcctcgatg cgggccaatt tctcgtcggc
     3481 cgaggcctct tgagtcgtca ctactgaggg agcatgtccc caaacgattc ggctgcccga
     3541 gtgtgtcacg attacgaatt cgtacttcgg cggatgagtg atctgggtgg atatcaattc
     3601 gatgtggcga aacccatatt tcttccaatc ctcgaagagg agcgccgcca actccgcagc
     3661 ctgaaccaca cgggcgtccc cccaactttg cccggcgggc ccagccggta acacgtccaa
     3721 ccccaccagc cgcgggtact gtgccgcctc tacgggcgta aaatcgcgag acggcagtag
     3781 caccccttct gcatcaacgg gcaaaagccc ctcgggtatt ctgaccatga ggaccggttg
     3841 ccgccacacc acacttgcgg agatcgtccc aggataaacc ttgtggagac tctgtacttt
     3901 tcgaatccat gggtggcgtt ccgcggccga cgcgactttt tgcaagaggt cctgctccag
     3961 gacgttacca ttcttttccg ggaacgagat gaggagtaat tctggcctaa tgtcgtgatg
     4021 aatccatgcc ggatattccc ctagattcag tgaatccggg gtcaattgaa aacggctcgt
     4081 ctttacgatt tcctgtccga accggtccca aaaaatgttc gccgccacaa atgaaacaac
     4141 gaacaaactc gcccatagga ggaaagatcg aatccacaat cggctgctat gagctgttcc
     4201 agcttcaccg tgaacggtgg tcttcttttt tttcgattta gctgatctaa atcccaagac
     4261 catgcgacgt gtttacatgt gtcaatcgaa aaaagcgaaa tctggcgacc aacgacaccc
     4321 caatggcact tatcgaacaa gtgcacggca ggccatcatc aaaacggtgg ctttgattaa
     4381 accaggcgcg atcgctacca aatgggccga cgtcggttcg tgccgcgagt gtcctttttt
     4441 ctattcgttc ccttatctcg cgggatgtca ccagatttcc aaatcaagtt cgagattaac
     4501 gcccaagcga ttgagcacct gcgtgcggat tgtgtcaatc agccgaagca tatcggttgt
     4561 ggtgcaattc ggttccgtga cgataaagtt agcgttgcgc tccgaaacca ccgcgccccc
     4621 gatccgggta cctttaaggc ccgctgcctc gacaacctcg gccgccgtgg tgccgcgggg
     4681 gtctcgaaag acccgcactg acgcttggtg ccccatgggc tgggcagcct ttcttacaat
     4741 ccacagtttt tggaggcggc gagccagctc atgcgggtcc tccggttcaa gttcaaactt
     4801 ggcctgtagg atgatgagat catcaagact gcttcgccgg tatccgaaaa cgacttccgt
     4861 gcggtcttta acggagagtt ggcctgtata atccagcacg gttacttcct tcacccattg
     4921 gccgatatcc cctccatggg ctcccacatt tcctttgaga gcaccaccaa tggtaccagg
     4981 gatagcagtc aacacttcca ggccggccaa accctcgtga acagccgttg tgattacccg
     5041 tgagagacta gcaccaccac cagcagaaac gatatttccg tcaattgtga catcagcaaa
     5101 atagggatgg ctcagttgga tgaccaaccc cggaacgcct tcgtcccgga tcagtatatt
     5161 tgaaccgctt cccaagactc gaatggggat cccctgttcg taggcacatc gcactatgtc
     5221 agccagttca tcagcgttac gaggctcagc ataaaactct gcgggacctc caaccccaag
     5281 ccacgtgtgc atggctagtg gttcattggc ccgaatggtt cgttccagac ctttccattg
     5341 cggcattgtc agctcctcaa tccggtacaa tccttgccca cagcctatga ttttaccgaa
     5401 gtgctcgccg tctggcgact cgaagaatct cgcgccgaat cccgcctaaa aacgttacac
     5461 ctcctcacgg agtgttacgg ccgcttggtt tcggttacga taagagggta tttcgtcgtt
     5521 aatgtcgcaa ggacacatct accttggctg gtccgttaga gcggcccggt tattgccagg
     5581 tatttcaggc gtaatccatc tagaatcgag tcatgagcaa gatgattgct gggacatctc
     5641 ctcgctggtt caatttgcct aattgtctaa cattactgag gatccttcta gctgtcgctc
     5701 tttttgttac gctgtccatc cacgagcgag ttgggtatgg cgtgtccatc gggttattta
     5761 ttgtggccgc tgcaacggac tggctcgacg gttttttggc acgccgtttg aacctcgtaa
     5821 cggcacttgg tcgaatcctt gatccattcg ccgacaagtt gatcatctgc ggaacattcg
     5881 ttttcttggc cgcaaccccc aagatgatcg aaacgccctg gggacttcat ccatggatcg
     5941 tagttgttgt catcagtcgc gaactcctca ttacgggtct gaggagcttt ctggaagggc
     6001 aaacggtgga tttttcagcc aaattctcgg gaaaacttaa aatgggcttt caatgcatca
     6061 cggccgttgt agccatgatg tatttggcag ataccggtta caacacgcac ttgtcctatc
     6121 ttctatggtt ggcgctggtt gtgtcaactt atttgacgct cctgacaacg gtttattccg
     6181 gtatcgccta tatcatgatt ggttggaaga aggttcggga tctcgaagct gccggatgaa
     6241 tctctttgta tggcttcagc agtatttggt gcgttggtgc tagtcacctt ggtttggtgg
     6301 gctgttgttt tcacgtcccg ccagacccgc cagatgcttt ggcctcgcta ccgctttcga
     6361 caattgccct ggcgtattcg agatctttta ctggtttggc ttttgttcct tttggctctc
     6421 gctgtttcaa ctgtcgtctc ccctctttcc acggcaaaag aaggagcgcg cgtttaccag
     6481 aatgagcccc aacgacacgg cctagattcg ccgggccgtg acgaagacga aaccacccag
     6541 ggccaacact gggtcttggt tctgatccaa tccgaccgct cgctgaaaac ggtcctcttg
     6601 tgtttgctgg gcaccggaat cctcgggccg attgcggagg aaatcctctt tcggggtttc
     6661 ctccagggat ggagccaccg tgtggaatgg gaacatcgcc gcacccgctt gaatatagca
     6721 cccattcgac gccaattacc tttgggcgca gcatccgttg cactggttgc ggttcttttc
     6781 gcaacagtcc attatcggcc ggacgagccc tccccgagtg ttgatgtgat tcgcgtgatc
     6841 cttattcgac agacactcgc ttattcgctg ttcctgccgg ttgccctggc cattgtcgca
     6901 cttaaagaca agccgcgagg actacggttg atttacgatc ggcggtggtt cttttgcgac
     6961 ataacccttg gagccggttg gtttttggcg tggatcgtcc cagtgtactt cgtgcaaggt
     7021 gctctcaaga cgatcctgcc ttggaaggcc atggccgatc catgtaccat gatcgtcatc
     7081 ggcacaatcc ttgggacact ctttgcccga acggggcggc tagcagccag tatcgtcttt
     7141 catgttctct tgaacatcac gagcctatct ttggcttttc tgatgtgatt cgcgggattt
     7201 gcgttaggaa atagacgaat gcgttcgaac gtttcaacat tggccaattg ggaaccggct
     7261 cgaaaagcac tgaaacggag ggtgagtgac tttaacggga ggcggcgatt ccgtataatc
     7321 gcgtcagtcc tttttcagat tgattcagcc cgaaatcctc gccgcaacag agcactaatg
     7381 ggcgtttgtc gagatttact ggaaaaacca aagcgagaaa ttggtttaaa ttgaagaagg
     7441 agacgtgaga tggccttggg cttcggtatc gttggttgtg ggttgatctc aaaatttcac
     7501 gcgcgtgcca tcggagatgt ccccgatgca aagcttgttg cctgttttga cgtggttaaa
     7561 gagtccgccc aaagactggc gcaggagttt ggttgtcgcg cgtatgacaa cttggatgcc
     7621 tttctcgccg atccggaatt aaacgtggtc acgattgcca cacccagcgg ggcccacatg
     7681 gaaccggcgg tggcagccgc tcgggcgggt aaacacgtca ttgtggaaaa gcccttggaa
     7741 atcactctcg agcgatgcga tgccattatc aaggcatgcg aagaagcgaa ggtggttttg
     7801 tcaacgattt tcccgtctcg ctttcatcaa tccagtcaat ggctcaagaa ggctgtggat
     7861 cagcaacgat tcggacgtct cacgttgggt gatgcctacg tcaagtggtg gcgaacccag
     7921 cagtactatg acagtggggc gtggcgaggg acttggcagt tggatggtgg cggcgccctg
     7981 atgaaccagg ctatacatag tgttgacctt ttagcctggt tgatggggcc ggtcgtcgag
     8041 attcaggccg ccaccggact tttagcacat gaacgcattg aggttgagga cgtggccctg
     8101 gctattctca gattcagcaa cggtgcactg ggggttttgg aggccaccac cgcggcctat
     8161 ccgggacacc tcaaacggat cgaaatccac ggtaatcgtg gttcggctgt gatggaagaa
     8221 gaggacatta tcaagtggga ctttgccgag gaacatcctg acgatcaggt gattcgtgaa
     8281 aagatggcct ccaggatcag tgggggtggc ggggcgtccg accccgcggc cattgggcac
     8341 cacggtcacg cacggcagtt tgccgacgtg gtccgtgcca tcaaagaggg caccaggccc
     8401 ttgatcgacg gttacgaggg tcgccgttcg gtggaaatta tcttgggggt atatcaggcg
     8461 gcggaacttg gccgggtcgt taaattgccg ttgccgggag atcccgatct tgtggcgaga
     8521 aaggtcggag ttgcgaatct gcgcaaggtt tgagtcatgt caaggagaat tcccttgacc
     8581 tagtgcgcag gaagggactt cgttgtccgg ccgccgcggg gcttgccgca ttgacaataa
     8641 ttgcaagtaa gaaacgtcaa ctcgtatttt tcgttcgttg gcctttggca cgagttcgtt
     8701 atccgatact gccggaactt tgcaagcctc tttggtttac aaatccgaga agtaactgtg
     8761 ttgcgttgtg gatgtgtctg ccagcggatg ataaggatgg atctttatgc caattgaatt
     8821 tgctcttccg aaccttggcg aaaatgtaac atcgggtgac gtcatcaatg ttcttgtcaa
     8881 agaaggggat gtcatcgtcg gaaaccaggc agttatcgag ttggaaacag acaaagccgt
     8941 ggtggaaatc ccctgcccct atggcggtca agtgaaaaaa gtcctcgtca agaaggggga
     9001 taccgtcaag gtaggtcaaa ccattttgat cgtggaaagc gaggttgcag agaaagctcc
     9061 ggtcgtgtcc ccagccaagg gaccggagtc acaggtggcc gcggccccgc ccgaagaaaa
     9121 acccagtcca ccacctgccg ccccgactcc gcctcccgta gcagcggtaa cggcagcaac
     9181 gacagcaacc gctgtggctc ccgaaccctc acccctagta gaggtggaca gccgtcttct
     9241 ccccgctggc ccggcagcac ggcggctggc ccgggagttg ggtgtcaatt tggcaacagt
     9301 tcagggaacc gggcgtcatg gtcggatcac ggttgaagac gttcaacggg ccgcgcagag
     9361 ccgggcagag gctccccgtg tcactattcc gagtcccgtc gtcccgccag gagagcctgg
     9421 gcaagactct tggggcccta tccgccgcga gaaaatgagc aaaattcgca agaccatcgc
     9481 ggcacagatg acgcgatcgt ggacaacgat tccacgggtc accaatttcg acgacgccga
     9541 cgtaacggat ttggagaatt tgcggaagtc ggtcccgccc gatttcctag gtgaagggtt
     9601 gaaattgact ttcatgccct tcgtcatgcg ggcagtgagc ctcgccctcc gtcatcatcc
     9661 ccttctaaat gcctcgctag atgaggaaaa cgagcagatc atctacaagg aatacatcaa
     9721 cttggggatc gccgtggata ccccacgtgg gctggtcgtc ccagtcgtgc gaaacgcgga
     9781 tcagatgacc ataccgcagt tggcacgggc actgagcgaa gtggcacgaa aagcgcgaaa
     9841 cgtagagttt accgtggacg aactgcgggg tgggacattt acaataagca acctcggtgc
     9901 tgtgggcgga acgtacagca ctccaattat caactatccc gaggttgcca tattactttt
     9961 gggtcggtcg cgttggttac cggttgttcg cgacggacag attgtcgccc ggtatatgat
    10021 gccgcttagc ctgtcctatg atcatcgtct ggtggatggt gcggcggccg cgcgattcct
    10081 caacgaaata atcgactggt tacaaaaccc tgggaaattg ctcctggtca gttagtttta
    10141 agaatggcgc cagagcttaa cagaacaatt tggcggtgtt gatagcagac gcaccccatt
    10201 atcggtgtgc ggcggtttgg agacggataa agcatggata tcgagctcgt tgttattggc
    10261 ggtggaccgg gtggatacgc ggcggccttt ttggcagccg atttgggcat gaaggttgcc
    10321 attgtcgatc aagaaccccg attgggcggt gtctgtctgc tccgtgggtg catcccttcg
    10381 aaggccttgc tccatgtcac ccgggtgatt gccgaaacgg aggagctaaa ggaatggggc
    10441 gtcgattatt ccaagcccgc cgtcgagctt aataaactac gtgctcgcaa ggaaaaagtc
    10501 atcagcattt tgaccggtgg attgaaacaa cttgccgcga aaagaaacgt gcgggtcatt
    10561 catgcccggg cgcagtttcg tgatggccag acccttcggc tttcgcccgt caatggcggt
    10621 accttggaag acaacgtctt gcgattccag cggtgtatca tcgccacggg ttctcgtccg
    10681 gtgattcctg gccttttccc caccggcgtc gatcgcatca tggactcaac ggttgctctc
    10741 gacctgcctg aaatccccga ttcacttctt gtcgtcggcg gcggatacat tggtcttgag
    10801 atgggaatgg tctatgcttc gctaggtagc cgggtcaccg tggtcgagat gatggacgga
    10861 cttcttccgg gtgttgatcg ggacctcgtg cgcccactcc aaaagcgact ggaagggctt
    10921 tttgccgcca tctatttaaa caccaaggtg gctgccgtac accttgtgga taacggagtc
    10981 gaagccacgt ttgaaggggc caaaactgga accgagcgat tctctcgagt tcttgtctcg
    11041 gtgggacggc gtcccaatag cgacaacctg ggcttggaga ataccaaagt ccaagtcgat
    11101 tcgcagggtt tcatcgttgt cgatgcccga aggcgaacgg ccgagcctgc cgtgtacgcg
    11161 attggcgatg tggcaggcca gccgatgctt gcccacaaag cggcccatga gggtaagaca
    11221 gccgtcgagg ccatttctgg gcacaaagtg gcctttgaac caagggctat ccccgccgtg
    11281 gttttcaccg atcccgaaat tgcctggacc ggattgacag agacagatgc caaacgcaag
    11341 gggattccgt acgaggttgc cgtatttcct tgggcagcaa gtggccgggc gcaggcaatg
    11401 ggaagaacag atgggttcac caagtggatt attgacccca agacggagcg cgtcatgggc
    11461 tgtgggatcg ttgggcccag cgccggcgac ctaatcgggg aagcgacgtt ggcggttgaa
    11521 atggccgctt cggctcgaga ccttgcggag acgatccacc cccatcccac actctgcgag
    11581 accttaggat ttgcggctga ggtctttttc ggacaagcga ccgacctcta tcggccacgc
    11641 aaaaaggatt aaggcagatt tgcaattttt actcgggccc acatttcgca gcaaattagc
    11701 cacggctgcc ccatcgctcc ccaaaaaatc tccccgcccc aaacagcgca acccatgcgc
    11761 acttcgaccg tggcgaaaaa cctggtggca taaacgctgg atcaggtttc ttccggacca
    11821 aaaggccaca cggccatatg ctcgctttgc cacgtcttct ttaaagcaat ggtgtggccc
    11881 ggcactggtc gattcccatc acccttttcc ggtcgattct cgccaaaagc caagaatgcg
    11941 gcaccacaat ggtgacaagt aacgtatttc cccaggtaat cgagccgaat ttggaccttc
    12001 cgaccacatg tcggacatcc ctgaatgaag tatccgtacg ccatccgtcg cacctcctcg
    12061 aaatttcctt gctggtacga tcctcacaac cggtatggta ctcgctcggg ataggtctgg
    12121 caagtatctc ggacagcaat ttagctaaag tccttcgctt cgacgtaggg ccaatcttat
    12181 ccacgaacag ccaaacgacc ggaacgcggc cacgcgctgt gatagggaac cacaggccgg
    12241 gcttggcggg gtccgcgctg gccctcccgg aaatatcatg ccgcatgtat tggccgccca
    12301 acgggcacga gagcgtgcgt cgctccagtc atttggttat gaatggccac gcgctgtggt
    12361 ggccagatca tgggattggc ttgctggtcc agatttgggc ggtaaatcga gccggcagtc
    12421 tagatcgggg cctgatgttc ccacaagccc gacggctgtt cgtgccgcta tttctgagta
    12481 aagggacgcc ttgccgggaa cacaccggat gattggtgtg ccgctggtac agtccccgcc
    12541 gccgtccctc tttggacctt gcgtaatttc gggaatcatt cacaataaca cttattggcc
    12601 acagtgggga aaatagggca acaattaccg gcaaacgtgg tggaccgcct cctgagaaat
    12661 tgccaccttg gggtggtatg atttgccact ggggagggcc attcctcgga tagcggttac
    12721 ccggtctgac ctatcaaagt ttgaatgcaa ctcgtttttt gagatttgaa aggctgcgat
    12781 gcttggacag gataatagtc gaatttgggc gagatatttc gcattcttgg ccatctctat
    12841 ggcgattatc gtcgtcaata tttatctgtc ttccctcttc tcgccccaac cgaacaaggt
    12901 acagccgccc ccggcggaga aggtcgtcgc ggacaatgcg tcgggcgaaa taaaaggcac
    12961 ccaaccgagc tcggagccca aaaccgagga cggtcccaag caacccagtg cctcggaaga
    13021 agaaacggct gtttcgcaat ccacggcctc tcggcaagaa gaaaagaaag agcccgacga
    13081 atcgcctcgt tcactcccca aaccgcctac tcggtacctc acgctgggtt cgctcgatcc
    13141 ggcgggacca tatcggatgt tgatcacact gtcgaaccgt ggagcgtctg tggtctccat
    13201 cgagatgagt agcctccgat actgcgatac cgagcagccc ggtggttatc ttggggccat
    13261 tgccatcgat cctgcagctg gtgaggacgg cgttcgagtg gatgtggtcg gtccgggaac
    13321 ccccgctgcc gaggctggat tgctacctgg cgacgtgatc accgaaatcg aaaatcagag
    13381 ggtgtacgac ttgcggagca tggaggccat tctttcacgc tgccggccca atcagtccgt
    13441 aaaaatccgc gtccggcgcg gcgaaaaaga gatggtcctc gatgctaggt tgcggcgtcc
    13501 accactgaga ctgattcgac gcgaggccgg tgcgcctttc tcctttgaga tggccctcgc
    13561 cctagcaaac aagacgcgac ttgccgacct gcgtgcgcct tttgaagaac gcatggcgga
    13621 agtcaaagac cccatggaaa aggagctcct ttatttgcag tggctgcatg ccgagattcc
    13681 cgagctggga atgcgttcgg aaaactggca tatcctcgcc gacacgagtg atccacgtcg
    13741 ccctgaaaca tggggtaaca cagaaattga ctcgatccct caaccgcgag aatctgtgcg
    13801 atttggcatt taccatgagc gactggggct agttgtctta aaaacataca cattgacaaa
    13861 agtcccggcg gacaagcaaa ccgaccccga taacccaagc taccatttcg acctgacaat
    13921 cgacgtcatt aacgtgggga aagatgctca agaaatagca tatgaattgg acgggcctaa
    13981 cggtctgccc acggagggag cttggtatgc ccgcaaaatg agcaacagtt ggagcgccgt
    14041 tggcctacgg gatgtggcgg ttcgaatggg taaaggtgcc gttcaattgg tcgcatgtcc
    14101 ggctattgtt aaggacaaat ggggcgtccc ttggaaagat gcgagcgaaa caatcaccgc
    14161 cttgggcgta gatgcccagt atttctcggt gatcatgctt cccaagaggt cacgtcttga
    14221 cgaaccctgg ttcgatgagg cccgtcctat tcgagtgggc ccggttgaac ctgattatca
    14281 gttcttaact aacgtttcgt gtcgtgttca ttcagtcccc acggttctcc agccgggtgg
    14341 ccaattgacc cacgaatata ccatattcgc gggaccgaaa cgacagaaat tgcttgccac
    14401 gtacggcctt cacggattaa tccagtatgg ttggttctgg ttcgtggcag accccatgac
    14461 gaaactcctg catctcttcc acaattactt ggtcttcaac tatgggctcg ccattatctt
    14521 actgacggtg atcgtcaggc tggcgatgtt cccgatcagc cgaaaacagg cccttaacgt
    14581 tcagaaaatg caggaacttc aaccggagat caagaaaatc caggagaaat acaaggacct
    14641 tcaggaacgg aaccgagcga cacaagagct tttccgacaa cacaa
//
LOCUS       DAOSUT010000098        14641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2481_length_14641_cov_8.769231, whole genome shotgun sequence.
ACCESSION   DAOSUT010000098 DAOSUT010000000
VERSION     DAOSUT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14641
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_14641_cov_8.769231"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLD05_09965"
     CDS             complement(<1..379)
                     /locus_tag="PLD05_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP77808.1"
                     /translation="MTRGLSVVIPVLNESPSLRQLYEEIRQVAEAHHYDYEIIFVDDG
                     STDDSWQVITQLAEKDARVKGLRFRRNFGKAAALMAGFSRTTKPFAMTMDADLQDDPA
                     EIPNFLAEIDKGSDLVSGWKKIRH"
     gene            complement(376..1458)
                     /locus_tag="PLD05_09970"
     CDS             complement(376..1458)
                     /locus_tag="PLD05_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOP77809.1"
                     /translation="MPVVALKRKTWKSWVIWGLSFLLVVLVIWATRRTLANALAEFNR
                     VRQEGGNLTISPGYASLAGLVYFFGFFPAAWFWYRVLNWLGQEVTFFRALRAYCIGQL
                     GKYVPGKAWVVILRTSLVQGEKVQPAMAAAGVFLETLTMMSVGGVLAVLYLIINLRGN
                     PQLFWLAVGLFVIVTLPTLPPFFRPILRFIARKGNREEVVVAIEKLGFARLAQGWLAM
                     VLLWTAYGLSLSLTLQSLGMKGAMMLSLLPNMVAAVSLATVAGFLVLFLPGGLGAREV
                     VLIAVLVPALGDQVVCSSGISSVELLAVIGAGLLRVIWLITEIVVAGVFWLTLVIGTF
                     FRLPGVTGRLQKDAPSPEPCGIEAQK"
     gene            1958..5182
                     /locus_tag="PLD05_09975"
     CDS             1958..5182
                     /locus_tag="PLD05_09975"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOP77810.1"
                     /translation="MKGSWILILAMVLPSSLWASDDLVLVENGHPRAEIIIADRPARM
                     ARMAAHELRETIEKISGARLPIVTAPTGKAVKVFVGASKHNPITADGLRYGAYRIASG
                     PDWLALIGDDTDFEPTEPFARSNSDIPRAQAEWEKIVGAPYGMPNAGLYKHRLKLPGD
                     IGKPDGAVTEKNEAFEVWGLDERGSYNAVCDLLRRLGVRWYMPGELGEVRPSLKTVKV
                     PRINETVWPDFSLRQMNFRFGVVGAETARWAMRLGLRNDERLQIAHGMSTMTNRQAVF
                     DAHPEWFALYGGRRDFRPGDSKCQLCYSNQELFQDTVRWARAMLDTYDFETVSIMPPD
                     GYVAICQCEQCRGKDTPERGERGLLSDYVWDFVNRVAREIAKTHPRAKVLNCAYGAYT
                     LPPLKIEKLEPNVVVCIVGGRRPFNRGGPKGEGEAAPEALRAAWAKKTDNPLLIFENY
                     PFTSRGWYLPAFDARTLGETVNATKGLSRGEDVWLSIGQDFHQKDIGFNHFLVYFTAR
                     AYWGGKEYDPDAELREYCRLFYGPAETEMLTFFDYCAANWRDMENDRAKIEQALALFE
                     KAKAKAETASDSVYRRRLALIDDFLKGVRMKSRQLGQKRGPLPVVRLVGDASGIVIDG
                     KLDDPYWQNCPSAATGRLRELQTGGAPVFGTTFKVGWQGNSVIFGIRCNESTGDVPLP
                     AGEFLRQPHPENRPISASARDDDPATWYGDAVEILIATESHSYYQIAITPAGHVADLD
                     RALPRDQWFAWDSKAEVATNIADDHWTVEIRLPVTEDENDPLHQILGRKPTQSLPWFV
                     NICRQRIRPSGAEYSAFSPTGTASFHEPLKFAYFYDGRSHQFDFDPEVTDFALQFRAA
                     TEQRKAEPFLALAAGRVTDFQKSAALEQAVRYDIAAAPTVIEKIPIEAVKKAAHMRYL
                     LAISRPGEVIARYAEEDFMSWPFWKRGDGYHARGRAYFMTKSGEKAERDLLAALQWTS
                     EPRTRDAILLLLAQNREQNLHNDEAALAAYRAIIDSRQKVGGADEYAALQGAARILSR
                     RGFFDEALRILNRAEPEKLQGTWRERIMKSISEIERAKETSTRP"
     gene            complement(5220..6500)
                     /locus_tag="PLD05_09980"
     CDS             complement(5220..6500)
                     /locus_tag="PLD05_09980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP77811.1"
                     /translation="MNYVLPLVTILVMVGSKTLCAEEPPEVRFMAMSPEEVRAYELDV
                     MRRVADLALIPPVLNTSPLPEYDYDKLDYGMTIGIERTPKGRIWACWVAGGDSPKAFF
                     VLATSDDDGETWSKPRLVIDSHSKNLPMDRTVIIGNLWTDPLGRLWLFFDQTMHHFDG
                     RAGIWATVCNNPDDARPQWSPPRRIWHGSMLNKPIVLSTGEWVLPIYLNQFPGFGPFR
                     DLFRELDPLRGAHMFVSTDAGQTWERRGGPVKFPNPDWIEHMFIERHDGTLWMLARTR
                     TGIMQSISTDRGRTWSEPTDLAGVAHPNARFFIRRLASGRILLVKHGDAIDSHQGRVK
                     LSAWLSEDDGKTWQGGLVLDDRPGVSYPDGFQAPDGTIYISYDRNRATDGEILLARFT
                     EADILAKKLVGPKSKLKILISKPLAPKKSASKVP"
     gene            complement(6497..8728)
                     /locus_tag="PLD05_09985"
     CDS             complement(6497..8728)
                     /locus_tag="PLD05_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP77812.1"
                     /translation="MSQRHQFVMATATTLLTFALFLALLLVPLPVRATEFYVSPDGND
                     ANAGTFSEPFASLERAQAALRAFRASNPHPTCRIILRDGIYRLEKTIVFQLGDAPVEI
                     VAASQATPILSGASPLPSEWQLAPPDLPRLPEKAWGHVWVADVPKEWPLFRTIFEGDT
                     MLPRARTAGFQQLSKAPEGSPAIDQHHLYLPVEVLNGIADFTGAEIVVVPRNPWVMNI
                     LPVTKVDRRSGLIFTDVPATYSLEPPRFGNFPNGTVWIENVLEALDEPGEWVLDVSAR
                     KLYFWPRNGKKPGHEIVVPRLTELVRVEGTIDYDGPTDIPVRGVVFRGLTFTQADRWP
                     WELNKTGWGLQHDWEMFDRPTAMLRFRGAESCRVEDCRFLNSGSAGVRLDLYCQKIAI
                     LRSEFSYLGGAGILLAGYGMGTKDVNRDNIVSDCHVHHIGRLLWHSAGIWAWQSGHNR
                     IEHNHVHHTPYTGILATGRTVLDRQGNGECSRTVRWAEVDRVLKAGRGTWYEREPLMH
                     ARENIIAYNDLHHCMEVMGDGNAIYVSGAGKNNIVRNNFIHDIPSPNINASIRCDDDQ
                     HLTVIENNIITRVCGEGIIWKGTNTIRNNILFDIRDVTPDGTPCLHKRGYLVMPYGPV
                     DGSVVERNIVVSCTPGQALLFERTTASKGQRTLLPAMLRTCKADYNLYFNTAEPGWGQ
                     KHLDAQRQFGIELHSIESDPKFRDPANDDFSLAPDSPALKLGFQPIDISTVGPRKVKG
                     NKP"
     gene            9438..9635
                     /locus_tag="PLD05_09990"
     CDS             9438..9635
                     /locus_tag="PLD05_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77813.1"
                     /translation="MTLLDWLRKLGILRYGAEATVFHNAKERPLSFQEDDVLNSQKDV
                     VRFGKKDELTNKPEGRKAKTT"
     gene            10397..11749
                     /locus_tag="PLD05_09995"
     CDS             10397..11749
                     /locus_tag="PLD05_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77814.1"
                     /translation="MKDLSLHGNRRQFLTGTSALALGAAVAGQVPAVHAGEDNTIRLA
                     LIGCGGRGAGAVGDALSTRDMGPIKLWAMADIDQERMEACYKALREKFGDDIDVSPER
                     RFTGFDGYKKAIDLLRPGDIAMCTTRAYIRPLHVEYAVSRGINVFMEKPFAPDPVGLR
                     RLLKAGEEAEKKGVKIAAGLQCRHSPARAALIEKINSGELGELICVRANRLGGRSWLG
                     NVGPKANSFLEQLKFGRIHLFWVGSGHFADYLIHQIDECCWLMGSWPVSCHGMGGREV
                     GSTDRGQNLDIYSLEYTFADGRKAFCGFRRALNAHNEFATFVHCSKKAGQFSGDVHAA
                     TVHIFKDQRIDKNNIEWSPTPDQYSPWVYEWRDFITSIRTDRPHNECKRAVYSDFATL
                     MGRAAAHYNRIVTWDEVFNSQFQFCNNLDQLNYDSPPPVTADENGYFPAPVAGQWTEL
                     "
     gene            12041..12685
                     /locus_tag="PLD05_10000"
     CDS             12041..12685
                     /locus_tag="PLD05_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOP77815.1"
                     /translation="MIMLPSIRFTFWLWGIGLIAAFCSGCTSTHADRGALFGGLLGAG
                     TGAIVGNAAGNTLAGTAIGAGVGALAGSAIGDSLDEMEARNRALIAEQMGRDIPRGQV
                     TYEDVIAMTRANVDEELIINHIRINGMSRIPSSDDIIRLQREGVSVKVIKAMQEAPIR
                     RSEPSDRPIIVREPAPPPVIIYERDPWWWGPPRWYRPYYYHPRPGVSWGITFHN"
     gene            12735..12878
                     /locus_tag="PLD05_10005"
     CDS             12735..12878
                     /locus_tag="PLD05_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77816.1"
                     /translation="MEKIRLAADPGKAGVLARLELGEGTRVIARGYGGARCPLTLPCL
                     PVS"
     gene            13002..13376
                     /gene="rpsL"
                     /locus_tag="PLD05_10010"
     CDS             13002..13376
                     /gene="rpsL"
                     /locus_tag="PLD05_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOP77817.1"
                     /translation="MPTINQLVRNPRKPKRKFSKAPALDGCPQKQGVCLVVRTMTPKK
                     PNSALRKIARVRLSNGKEVTVYIPGEGHNLQEHSIVLVRGGRIRDLPGVRYQVIRGTR
                     DCAGVEGRRQARSRYGAKKPSK"
     gene            13448..13918
                     /gene="rpsG"
                     /locus_tag="PLD05_10015"
     CDS             13448..13918
                     /gene="rpsG"
                     /locus_tag="PLD05_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOP77818.1"
                     /translation="MGKKTASEAILKPDPKYGSLLASKFINCMMWNGKKTVAQEIFYK
                     ALDILATKFPEKNPIDVFTEAVENVKPNIEVRSRRVGGASYQVPMQVNAKRQQSLAIR
                     WIIQAARERKGKPMYERLAAELEAAYKKEGAAITKRENVHRMAEANKAFAHFAW"
     gene            14080..>14641
                     /locus_tag="PLD05_10020"
     CDS             14080..>14641
                     /locus_tag="PLD05_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOP77819.1"
                     /translation="MPRDIRSIRNIGIVAHIDAGKTTLTERMLFYAHLIHRPGSVDEG
                     TTVTDYDPEEQERGITIQAACVTFDWRGYHINLIDTPGHVDFTAEVERSLRVLDGAVV
                     VFSAREGVEAQSETVWRQADKYHVPRIAFINKMDREGADYYGTLEEIEKRLECRPIPV
                     NLPIGSGPAHFDNAFCGIIDLISMKAL"
BASE COUNT         3331 a         3834 c         4288 g         3188 t
ORIGIN      
        1 catgccgtat ctttttccat ccgctgacaa gatcagaccc tttatcaatc tcggcaagga
       61 aattggggat ttctgcggga tcgtcctgta aatcggcgtc catggtcatg gcgaacggtt
      121 tggttgtgcg agagaacccc gccatcaggg ccgccgcctt cccaaagttt ctccgaaagc
      181 gaagcccctt gacccgcgca tccttttcag ccaattgggt aatgacttgc caactgtcat
      241 ctgtggaacc gtcatcaaca aaaataatct cgtagtcgta atggtgggct tcggcaactt
      301 gcctgatttc ctcgtaaagc tgccgaagac taggcgactc gttcaacacg ggaataacaa
      361 ccgatagtcc gcgcgtcatt tttgtgcctc aataccgcaa ggttctggcg aaggggcgtc
      421 tttttgaaga cgacctgtca cgcccggaag ccggaaaaat gtcccgatta caagggtaag
      481 ccaaaacacg ccggctacca cgatctccgt aatcaaccaa atgaccctta ataatcctgc
      541 gccaatgacg gccaataatt caacagagga gataccagac gaacagacaa cctggtcgcc
      601 cagggcgggg actaacacgg caatcaaaac aacttctctg gctcccagtc caccgggaag
      661 aaacagcacc aaaaacccgg ccaccgtcgc cagcgatacg gcggccacca tattcggcaa
      721 cagggaaagc atcatcgctc ccttcatccc cagtgactga agggtgagac tgagactcaa
      781 tccgtacgcc gtccagagca acaccatcgc taaccagcct tgggcaagtc gggcaaaacc
      841 cagcttctcg atggctacaa caacctcctc gcggtttccc ttgcgggcga taaaacggag
      901 gatgggccta aagaaaggag gtagggtagg taacgtcaca atgacgaaca gtcccacggc
      961 aagccaaaat agttggggat ttcctcgcag attgataatc aaatacagga ccgcgaggac
     1021 tccacccacg gacatcatcg tgagcgtctc taggaaaacc ccagcagcag ccatggcggg
     1081 ttggaccttc tcgccctgta ccagggatgt ccgtagaatc acaacccaag ccttccctgg
     1141 gacatacttg cccaactggc cgatgcaata agcccggagt gcccgaaaga atgtcacctc
     1201 ttgcccaagc cagttgagga cgcggtacca aaaccacgcg gccgggaaaa acccaaaaaa
     1261 atacaccagg ccggcgagcg aggcataccc cggagaaatg gtcagattcc ccccctcctg
     1321 gcgtacacgg ttaaattcgg ccaaggcatt cgccaatgtc ctgcgagttg cccaaatgac
     1381 gaggacgacc aagaggaatg ataaccccca gatcacccaa gatttccatg ttttccgttt
     1441 tagtgcaacg actggcaaga tcgtctctcc gaagttaccg taggcaggca gccccacgcg
     1501 agaatgttat ctatcaggcc tgtggtacca cggcgctcga ttgtagcttc tctattttcc
     1561 ctctctttcg gaaaaggaac cgacggaacg accttgtggc tatcttatcg cccatcgtca
     1621 tttcccaaaa gggcatcgcc ttggtgtaca cccagtttgg cggacgaaag gcgtttgcgg
     1681 caaagagtgg tttcgctcaa agtcagttgg aagtgggcgc tcggagcgga acagtcgagc
     1741 tgcccatttg tcgcggtgtt ccgttcgcgg tgcgtacatc aattttagga aacgtttggt
     1801 gggtcgggat tccagaaggc atcccagcag gaagagggag taatggccag gagaaaaccc
     1861 ctttgacatg aaaaggtcgg tggaaatgtc cccggacgcc agctaacatg gcgttaggct
     1921 tggccgatga gtgcatgttt attgggagcg acaacatatg aagggctctt ggatattgat
     1981 tttggcgatg gttttgccat cgtctctatg ggcaagtgat gacctggtgt tggtcgagaa
     2041 cggacatccg cgggcggaga ttataatcgc ggaccggccc gcacgaatgg cccgtatggc
     2101 ggcccacgaa ctacgcgaga ccatcgaaaa gatcagcgga gcccggttgc cgattgtgac
     2161 cgcgcccacg ggcaaagcag tcaaagtctt cgttggggcg agtaaacata atcccataac
     2221 ggcagacggg ttgcgctacg gtgcttatcg tattgcctcg gggccggatt ggctggccct
     2281 tattggcgat gataccgatt ttgagcctac cgagcccttt gcccgcagca acagtgatat
     2341 tccgcgtgca caggcggaat gggaaaagat tgtcggcgcg ccgtatggaa tgcccaatgc
     2401 tgggctttac aagcaccggc ttaaattgcc tggagacatt ggcaaacccg acggggcggt
     2461 gacggaaaag aatgaggcgt tcgaggtttg gggcctcgat gagcggggga gttacaacgc
     2521 cgtttgtgac cttttgcgga ggctgggggt caggtggtat atgccgggcg agctaggcga
     2581 agtgcgaccc tcgttgaaaa cggtcaaggt gccgaggatt aacgagacgg tttggcctga
     2641 tttctcgctg cggcaaatga acttccgttt tggcgtggtg ggtgcggaga cagcccgctg
     2701 ggcaatgcgg cttgggctac gaaatgatga acgcctccaa attgcccacg gcatgtccac
     2761 aatgaccaac cgccaagccg tcttcgatgc ccatcccgag tggtttgcct tatatggagg
     2821 aagacgtgac tttaggccgg gcgattccaa gtgccagctt tgttattcca atcaagaact
     2881 gttccaggac acagtccgct gggcgcgagc gatgcttgac acgtacgact ttgaaacggt
     2941 ttctattatg ccgccggacg gttatgtggc gatttgccag tgcgaacagt gtcgcggcaa
     3001 agatacgccc gagcggggcg aacgggggtt acttagcgat tatgtgtggg actttgtcaa
     3061 tcgtgtggcg cgcgaaattg ccaaaacgca tccacgagca aaggtcctaa actgcgcgta
     3121 tggggcttac acgctgcccc cgctcaagat cgagaaattg gaaccgaacg ttgtggtgtg
     3181 tattgttggg gggcgacgac cgttcaatcg gggtggacca aagggcgaag gtgaagccgc
     3241 tcctgaggca cttcgtgcag cttgggcgaa gaaaactgac aatcccttgc tgatttttga
     3301 gaactatcct ttcacatctc gtggctggta cttgcctgcc ttcgacgccc ggactttggg
     3361 tgaaactgtg aacgccacga aaggtctttc ccggggtgag gatgtctggt tgagtattgg
     3421 gcaagacttc caccagaagg acatcgggtt taaccatttc cttgtctatt ttacggctcg
     3481 tgcctattgg ggtggaaaag aatacgatcc cgatgctgaa ttacgtgaat attgccgcct
     3541 cttttacggc ccggccgaaa cggagatgct cacttttttc gactattgcg ctgcgaactg
     3601 gcgggatatg gaaaacgatc gagcgaagat tgaacaagcc ctggcgctat tcgagaaagc
     3661 gaaggccaag gcggaaacgg cttctgacag cgtctatcgc cgacgtttgg ccctcattga
     3721 cgattttttg aagggtgtgc ggatgaaatc acggcagctc ggccaaaaac ggggaccgct
     3781 ccccgtggtg cgacttgttg gcgatgcctc cggcatcgtc attgatggca aattggacga
     3841 tccctattgg cagaattgcc cgagcgcggc gacgggtcgg ctccgtgaac tccagaccgg
     3901 tggtgcgcca gtctttggga ccaccttcaa agtgggctgg cagggaaaca gcgtcatttt
     3961 tggcatccgt tgtaatgaaa gcaccggtga cgttccgttg ccggccggtg aattcctccg
     4021 ccaaccgcat ccagaaaaca ggccgatctc cgcttccgca cgcgatgatg acccggctac
     4081 ctggtatggt gacgctgtgg aaatcctcat tgccaccgag tcccactctt attatcagat
     4141 tgccatcact ccggcggggc acgtggccga tctcgaccgc gcgttgccaa gggatcagtg
     4201 gttcgcttgg gattcgaaag cggaggttgc cacaaacatc gccgacgacc actggacggt
     4261 tgaaattcgc ctgccggtca cggaggacga aaatgacccg ctccatcaga ttctcggccg
     4321 caagccaaca cagagcctac cgtggttcgt caatatttgc cgccaacgta ttcgccccag
     4381 tggagccgaa tactcggcct tttcaccgac gggtacggcg agctttcatg aaccactcaa
     4441 gtttgcgtat ttctacgatg gccggtcgca tcaatttgac ttcgatccgg aggtgaccga
     4501 ctttgccttg caatttcgtg cggccacaga gcagcgaaaa gccgaacctt tcttggcact
     4561 tgcggcagga cgggtcaccg actttcaaaa gtccgcggcg ctggaacagg cggttcgtta
     4621 cgacatcgcg gctgcaccga cggtaatcga gaagattccg attgaagctg tcaagaaagc
     4681 ggcacacatg cggtatttgc tggcaatatc gcgaccaggt gaggtgattg cccgctatgc
     4741 cgaagaagat ttcatgagtt ggccattctg gaaacgaggg gacggttatc atgcccgcgg
     4801 tcgggcctat ttcatgacga agagtggtga gaaggcagag cgtgacttgc tcgccgccct
     4861 ccaatggacc agtgagccgc ggacccgcga tgccattttg ttgctgttgg cacaaaaccg
     4921 tgagcaaaac cttcacaatg acgaggccgc cttggccgct tatcgcgcga tcatcgacag
     4981 ccggcaaaaa gtgggcggtg cggacgagta cgctgccttg caaggcgccg cgcgtattct
     5041 gagccgtcgg ggatttttcg atgaggccct gaggattctc aatcgcgcag agccggaaaa
     5101 acttcaagga acgtggaggg agagaatcat gaagtccatc tctgaaatcg agcgcgccaa
     5161 agagacgagc acccgaccat gatcggctgg aacccgttcc gagctgtcgg cttggcttcc
     5221 tagggaacct ttgatgccga tttctttggg gcaagcggct tactaatgag gatttttagc
     5281 ttggacttag gaccgacgag ctttttggct aggatatcgg cctcggtaaa tcgggcaagc
     5341 aggatttcac cgtcggtggc ccggttgcga tcataagaaa tgtagatggt cccatccggt
     5401 gcctgaaaac catcgggata ggaaacgccc ggtcgatcat ctaaaaccag cccaccttgc
     5461 caagtcttgc cgtcatcttc ggaaagccat gcactgagtt tcacgcggcc ttggtgggaa
     5521 tcaattgcat caccatgctt caccaaaagg atgcggcctg aagccagacg gcggataaag
     5581 aaccgcgcat tggggtgggc gacgcctgcg agatcggtcg gttctgacca agttcgtcct
     5641 cgatcggtcg agatcgattg catgatgccg gttcgtgtcc gcgccaacat ccacagagtg
     5701 ccgtcgtgac gctcaataaa catgtgctcg atccaatccg gattaggaaa cttcactggc
     5761 cccccgcgtc gttcccaagt ctggccggca tcggtggaga caaacatgtg ggcaccgcgg
     5821 aggggatcca attcgcggaa cagatcgcgg aacggtccaa aaccgggaaa ttggttcaaa
     5881 taaatcggca aaacccattc gccggtggat aacacaatgg gcttgttgag catgcttccg
     5941 tgccagatac ggcgcggtgg cgaccactgc ggccgggcat cgtcgggatt gttacacaca
     6001 gtcgcccaaa taccggcccg gccatcaaaa tggtgcatcg tctgatcgaa gaacagccag
     6061 aggcgtccga gcgggtcggt ccacaggttg ccaataatca ccgtgcggtc catcggaaga
     6121 ttcttcgagt gcgaatcgat gaccagacgc ggttttgacc atgtctcgcc gtcatcgtcg
     6181 ctggtggcca gcacgaagaa cgccttgggg ctatcaccgc ctgccaccca gcatgcccaa
     6241 atccgcccct tgggggttcg ctcgatgccg atggtcatac cgtaatcgag tttatcgtag
     6301 tcgtattcgg gtaggggtga tgtgttcaat acagggggaa tgagagcgag gtcggccacg
     6361 cgacgcatta cgtctaactc gtaggctcgc acttcctcgg gcgacattgc catgaagcgc
     6421 acttccggcg gttcctcggc gcacaaggtt ttagagccaa ccatcaccaa aatcgtgacc
     6481 agcggcagaa catagttcat ggcttgtttc ctttcacttt tcgtgggcca acggtagaaa
     6541 tgtcaatggg ctgaaacccc aacttcaggg ccggcgaatc gggagcaagg ctaaaatcgt
     6601 cattcgccgg gtcgcggaat ttcggatcgg actcgatgct gtgaagttcg ataccgaatt
     6661 gacgctgggc gtccagatgt ttttgccccc aacccggttc ggcagtgttg aagtagagat
     6721 tgtagtctgc cttgcaggtc cgcaacatgg ccggcaagag ggtcctttga cctttcgatg
     6781 cggttgtcct ttcaaagagc aatgcctggc cgggcgtgca ggagacaaca atgttgcgct
     6841 ccaccacgga gccatccact gggccatagg gcatgaccaa gtaaccccgc ttgtgcaggc
     6901 aaggtgtgcc atcgggcgtt acgtcccgaa tatcaaagag gatgttgttg cgaatggtgt
     6961 tagtaccttt ccagatgatc ccttcgccgc agaccctggt gatgatgttg ttttcgatca
     7021 cggtcaagtg ttggtcgtca tcacaacgga tgctggcatt gatgtttggc gaagggatgt
     7081 cgtgaataaa gttgttgcgc acgatgttat tcttgccggc gccggaaaca tagatcgcat
     7141 taccatcgcc catgacttcc atgcaatggt gaagatcgtt atacgcaatg atgttttccc
     7201 gggcgtgcat gaggggttcg cgctcgtacc aagtaccccg cccggcctta agaacccgat
     7261 cgacttccgc ccaacgtacc gtgcggctac attcaccgtt gccctgacgg tctaggactg
     7321 ttcggcctgt cgccaggatg cccgtgtagg gcgtatggtg gacatgatta tgctcaatgc
     7381 gattgtgacc actttgccag gcccagattc ccgccgaatg ccagagcaag cgaccgatat
     7441 gatgcacgtg acaatcgctg acaatgttgt ctcggttgac atccttggtg cccataccgt
     7501 agccagccag taagatgccg gctccgccga gataagaaaa ttcagaacgc agaatggcga
     7561 ttttttggca ataaaggtcc aatcgcacac ctgccgagcc gctattgagg aaacgacagt
     7621 cttcgacgcg gcaggactct gcgccgcgga accgcagcat agccgtcggg cgatcaaaca
     7681 tctcccaatc gtgctgcaat ccccaacccg ttttgttcag ttcccacggc cagcgatcgg
     7741 cttgcgtgaa tgtcaatccg cggaacacca cgccgcggac gggaatgtcc gtgggtccat
     7801 cgtagtctat tgtcccttcc acacggacga gctccgtcaa acgcggtacc actatttcat
     7861 gaccaggttt cttgccgttg cgtggccaga agtacagctt acgggcgctg acatccaaga
     7921 cccattctcc aggttcgtcg agggcctcaa ggacattctc aatccacacg gttccattgg
     7981 gaaagttgcc gaaccgaggt ggttcgagtg agtacgtggc gggcacatcg gtaaagatga
     8041 gaccgcttcg gcgatccacc ttggttacgg gaaggatatt cattacccaa ggatttcgag
     8101 gcaccaccac gatctcagca cccgtgaaat cggcaatccc attgagaacc tcgacgggca
     8161 agtaaagatg atgttgatca attgcgggac taccttcggg ggctttgctg agctgctgaa
     8221 agccggccgt gcgtgcccgt ggtagcatcg tgtcgccctc aaagattgtg cggaacaatg
     8281 gccattcctt gggcacgtct gccacccaga cgtgacccca ggctttttcc ggcagtcgtg
     8341 gcaggtccgg aggtgccagt tgccattcac tcggaagggg cgaggcacca ctcagaatag
     8401 gggttgcctg ggatgcggcc acgatttcga ccggggcatc gccaagctgg aatacgatgg
     8461 tcttttccaa ccgatagatg ccatccctca agatgatccg gcaggttgga tgtggattgg
     8521 atgcacggaa tgcgcggagg gcggcctgtg cgcgttccaa gctcgcaaac ggttccgaga
     8581 acgtcccggc attcgcatca ttgccgtcgg gagagacata aaactctgtg gcacgtacag
     8641 gtagcggaac gagcaacaat gcaaggaaca gtgcgaaagt caacaatgtt gtggcagtcg
     8701 ccataacaaa ttgatgtcgc tgtgacatac cagttgtcct cccctgatgg ttttgactgg
     8761 ccaagcggca gaggcctgct tattcggtag ccttcgtggc atatcaccgt tttcacttgt
     8821 ggacgcttgg ggacgcacgc gagccgcatt atagcaacca tcgtactgca aggcgaacct
     8881 aggcagccca agcggacgtt ggtggcccga ttcgtttgca ctgtctgctt tgaccaccga
     8941 ccaattatgg tcaggcctct cccctgcgca ccgaccactt cgcaatgtgg cgataccaat
     9001 ttatggttgg aatcggtcgt aacggtcaga tgggccatgc cgtacggccg aagccggcgt
     9061 gtccacagca tgctaggatt tgggtgtgaa tgtgccaaga gttccaaaag gttcttccgc
     9121 ttttagacga ctctacgata ggtgcttaac cgcgttcact tgggtgtgtt ggcctgggga
     9181 acaggatagc ttgcttggga tttagagtct ctcccataat ctcccctcgc ccgttcggcg
     9241 ggagaggggt cgggggtgag ggtggctcga atcactgccg gtgtcctcga tcaattccga
     9301 cgttccgttg ccctcacccg aacgttctcc cggagggaga gggggacctt tcgagatagg
     9361 ctcttaaccc tcggcgagcg gcagtctcac gggagcacac ggagaaaacg ttacagactt
     9421 gttccaggag aacgaaaatg acgttactcg attggttgcg taagctcggt attttgcgat
     9481 atggtgccga agccaccgtg tttcataacg caaaagaaag acctttaagt tttcaagaag
     9541 acgacgttct caactcgcag aaggacgtcg tgcgtttcgg caaaaaggat gagctgacta
     9601 acaagccgga aggccgaaaa gccaaaacga cctaacaaca cgcgaattga acattttgtt
     9661 aaatccgacg ttgctgccaa cttctgtcgg ctttttgtga gcggtatggc ggcgatgact
     9721 tgcgtttggc tagcaaagct tttcgcgcgt cggtgaatag ccgggctcga cctgcgaatt
     9781 tccctcagca cgagggctgt ggaggccgcc cgatcaaagt tccaccacgt tttcccgtcc
     9841 ccaggcttcg tgcgaagtaa cgattgcatg agcgggacgt ggatccatgc gaaacggagg
     9901 agaaacgcta acgtcaccgc cacttagcat cgggccgctg aacggagaaa gcgtgaaaac
     9961 cgagttggct ggggcccctc acgtagcgtg ggagtgttct gaaagaacac gatcaagagc
    10021 agcagtaaat tgcgggccga atctccactc atcttagcga cttcgatcat ccggcaaaat
    10081 tctgcgaacc tgacagaggc gatcatctcg ttttttccat ggaagtgtac accttccact
    10141 ccgcaatagc cccagatgct tgacggacaa ggggcagtct gacaatatgg ctatacgcca
    10201 agctgctgcg ccgttattgt gcgcgaggat tgcgattgcg caatgaggtt cttagcgtga
    10261 gctagtggga tagtctagaa aggggccaca agcgttacct aacggtggac gttgacccgt
    10321 tgttatggga tccaacgggt acccgtggat tgaggaccct gttaacaagc gtgatttgtg
    10381 cagaaaggtt ttagcgatga aagacctatc ccttcacggc aaccgacgac agtttttgac
    10441 aggcacaagt gctttagcct tgggggctgc agttgcgggc caggtgcctg ctgttcacgc
    10501 gggggaagac aataccatcc gattggccct tattggttgt gggggccgtg gtgcaggagc
    10561 ggtgggggac gctttaagta cccgcgacat gggaccgatt aagctttggg cgatggccga
    10621 catcgaccag gagcgcatgg aagcatgtta caaagccttg cgcgagaagt ttggtgacga
    10681 tatcgacgtc tcgccggagc gccgttttac gggattcgat gggtacaaga aggcgattga
    10741 ccttctccgg ccgggcgaca tcgctatgtg tacgacgcgg gcatacatcc ggccacttca
    10801 tgtggagtat gccgtgtcgc ggggtattaa cgtgtttatg gaaaagccat tcgcacccga
    10861 cccggtcgga cttcgacggt tgcttaaggc tggggaagaa gctgagaaaa aaggcgtcaa
    10921 gatcgcggcc ggtttgcagt gccgccattc accggcccga gcggctttga ttgaaaagat
    10981 taactcgggc gagttaggcg aattgatctg tgtccgcgcc aatcggctgg gagggcggtc
    11041 ctggttgggc aacgttggtc ccaaggccaa tagttttctc gaacagctca aattcggacg
    11101 gattcatctt ttttgggtcg ggtcggggca tttcgccgat tacttaatcc atcagatcga
    11161 cgagtgctgc tggctcatgg gttcctggcc ggtctcttgc cacgggatgg gcgggcgcga
    11221 ggtggggagt accgaccgtg gccaaaactt ggatatctat tcgctggagt acacgttcgc
    11281 cgatgggcgc aaggcctttt gcggtttccg gcgtgctttg aacgctcata atgagtttgc
    11341 cacgtttgtc cattgctcga aaaaggcggg tcaattctcg ggagatgtac acgcggcaac
    11401 ggtgcatatc ttcaaggatc agcggatcga caagaacaat atcgaatggt ccccgacgcc
    11461 cgaccaatat agcccctggg tctatgaatg gcgtgacttc atcacaagta tccgtacgga
    11521 tcgcccacac aatgagtgca agcgggcggt ttattcggac tttgccacgc tcatgggccg
    11581 ggcggctgct cactacaacc gcatcgtgac ctgggacgag gtctttaact ctcagttcca
    11641 gttttgcaat aacttggatc agttgaatta cgactcgcca ccccctgtca ctgccgacga
    11701 aaacgggtac ttcccggctc cggtagcggg ccagtggaca gagctttgat tgattggtgg
    11761 gaaaacggct tctgataatc gcatccggcg ggtctgaagc accgcaagtt gtgcggcccg
    11821 cctgttgtgc tgcccttgcc taggttgtga caacgtcgcc cattttcgct tgacgcttgg
    11881 gcgatggctt tttattctgc gcaaattgac attttcatcg aatggatttg gtcgggcgaa
    11941 aaaagtagcc gataaggtat gggatagaga atgtcaattt cccggcttat ttcaaaacct
    12001 cggcggttag ggaggacgtg cccagccaag gagttcacgg atgataatgt tgccatccat
    12061 taggttcacg ttttggctct ggggaattgg gttaattgcc gccttctgct cgggttgcac
    12121 cagcacgcac gcagatcgag gggccctttt tgggggccta ttaggcgcag ggacaggggc
    12181 catcgttgga aatgcagccg gaaacaccct cgccggtaca gcgatcggcg ccggggtggg
    12241 ggctcttgcc ggatcagcca taggagacag cctagacgaa atggaggccc gcaatcgggc
    12301 tctgatcgcc gaacaaatgg ggcgagacat tcccagaggg caagtcacct atgaggacgt
    12361 catcgccatg acgcgggcaa acgtggacga agagttgatt atcaaccata ttcggattaa
    12421 cgggatgagc cgaatcccct cctccgatga catcatccgt ctccaacggg agggggttag
    12481 tgtcaaggtc atcaaggcca tgcaggaagc ccccatacgc cgctctgagc cgtcggaccg
    12541 tccgattatc gttcgagagc cggcacctcc gccggtaatc atctatgaac gtgacccctg
    12601 gtggtgggga ccgccacgtt ggtatcgccc gtactactac cacccccgac ccggggtgag
    12661 ttgggggatc actttccata attaatcgat cgagtcgccc agagcttttc cgagtggtgg
    12721 ccgttcgagg aaggatggag aaaatccggc ttgccgccga tcccggcaag gcgggagtgt
    12781 tagcccggct tgaattgggt gaaggcacac gggtcattgc ccgaggttac ggcggggccc
    12841 ggtgtccctt gacattgccg tgcctgccgg taagttaaaa gattgttcac gctcggggta
    12901 gggttacccg ggagacgggt gcgcgacacg cgcccgagtt ttttgcttca cgcgagcccc
    12961 agtctaaagg ggggtaggag attttagaaa acggttgaaa catgcccaca attaatcaat
    13021 tagtacgaaa tcctcgaaag ccgaaacgaa agttcagcaa ggcgccggcg ctggatggat
    13081 gcccacaaaa acaaggcgtg tgtttggtcg tgcgtaccat gacgccgaag aagccgaact
    13141 ccgcattgcg taagatcgcc cgggttcggt tgagtaatgg taaggaagtg acggtatata
    13201 ttcctggcga aggtcacaat ctgcaggagc actcgattgt gctcgtgcgg ggtggtagaa
    13261 ttcgtgacct gccgggcgtg cggtaccagg tcattcgtgg tacgcgagac tgtgctggag
    13321 tggaaggtcg gcgtcaggct cgaagccggt acggtgccaa gaaaccaagt aagtagtacg
    13381 ggagtggtgg ctcggttttc ggtcgatcat ttcagcaagc aacaacgttt gttattggaa
    13441 aaggattatg ggcaaaaaaa ctgccagtga ggcaattctt aagccagacc cgaaatacgg
    13501 ctccctgcta gccagcaagt ttattaattg catgatgtgg aacggcaaga agacggtggc
    13561 tcaggagatt ttctacaagg ctctggatat tcttgccacc aagttcccgg agaagaaccc
    13621 gattgacgtc ttcaccgagg ccgtggagaa tgtcaagccg aacatcgagg tgcgatcacg
    13681 gcgggtcggt ggtgcgtcat accaagtccc aatgcaggtc aacgccaagc gacagcagtc
    13741 attggcgatc cgttggatta ttcaggcggc cagggagcgc aagggcaagc cgatgtacga
    13801 gcggttggct gccgaattag aagcggccta taagaaggaa ggcgcggcga ttacgaagcg
    13861 cgaaaacgtc caccgcatgg cggaggccaa caaggcgttt gcgcattttg cgtggtagct
    13921 tgcacgaatt tttcacaccg cgccgatcct ttcggtgcgg tgttcttttg cgcacaggcc
    13981 gcgattggtg ggggcttttg ggcaggacga tgggttgagg tgatagaata accttgccga
    14041 agataagctt ggcattttgg atacactggt gcgctgacta tgcctcgtga catccggagt
    14101 attcgtaaca tcggtatcgt tgctcacatc gatgccggga aaaccacgct cacggagcgg
    14161 atgctctttt acgcgcatct catccatagg ccgggttctg tggatgaggg gacgactgtc
    14221 accgattatg accccgaaga gcaagagcgc ggcattacga ttcaagcggc ctgtgtgacg
    14281 tttgactggc gtggatatca catcaacctg attgacacgc cggggcatgt ggattttacg
    14341 gccgaggtag aaaggtccct gcgggtcttg gacggggcgg ttgttgtctt cagtgcgcgc
    14401 gaaggggtgg aggctcaaag cgagaccgtc tggcgacagg ccgacaagta ccatgtgccg
    14461 cggattgcct ttatcaacaa aatggaccgg gaaggggccg actattatgg gacgttggag
    14521 gagatcgaaa aacgcctgga gtgtcgtccg attcccgtca atttgcccat cggcagcgga
    14581 cccgcacact ttgacaatgc cttttgtggc attattgacc tcattagcat gaaagcattg
    14641 a
//
LOCUS       DAOSUT010000099        14608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2490_length_14608_cov_8.990311, whole genome shotgun sequence.
ACCESSION   DAOSUT010000099 DAOSUT010000000
VERSION     DAOSUT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14608
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_14608_cov_8.990311"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1318)
                     /locus_tag="PLD05_10025"
     CDS             complement(5..1318)
                     /locus_tag="PLD05_10025"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOP77820.1"
                     /translation="MYLRVLPEVESVITAAELTLHTVKDLAEMAKKKGISGWRSMRKQ
                     ELIEALLNVARREARQRKAKQKSVTSKTSKGGRIKGQSSSGNGKVKRGQPADSSKASS
                     SAKSVDQKSSRTRSNSASLATSEVSGGTRRKVKAAPPSPEVQKQVEELRRELIDAKDL
                     ANSSREGSLGATKDRLVLMVRDPYWLQAYWEIARQTVERARAALGQNWHLARPVLRLW
                     EIRRDGTSHVERIHLRDIEIHGRVNNWYINVDHPPRSFEVDIGYVSPEGKFFCLTRSN
                     IVTTPLASAAGPDLDWAILADEYERVYALSGGYDEATDTSELREVLEDRLRRPLGGSV
                     IQRLGMGMEGQTEHRSRFPFHVDAELIVFGHIQAGYQVTIRGEPVPVGPDGRFSLRLP
                     LPDRRHVLPVVGQSPDGAEQRTIVLAVERNTKVMEPLLKENDPQA"
     gene            1933..2238
                     /locus_tag="PLD05_10030"
     CDS             1933..2238
                     /locus_tag="PLD05_10030"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOP77821.1"
                     /translation="MGHRQKRSTKDTRATWVMGYQISSQIITIALELVLPSLMGFFAD
                     RKWHTFPALTLVGVLIGFSAGTLHFVQFAKGLNHSRAKAAKNEQSRQFGEPKEPPHE"
     gene            2256..2768
                     /locus_tag="PLD05_10035"
     CDS             2256..2768
                     /locus_tag="PLD05_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77822.1"
                     /translation="MSGHQVKQINPPFLSTSCVDFARGTVVLAGVVGVISLIFAAVIF
                     LALRNPSGELLRLVLAVVVSGFGGMAGLAVLCFSRGTTWELWAPLAATPVRTAATLLS
                     LALLLVLPAGLVTTSFLLYLIVFYISILGCETILALQQLRKNTASIGSAVRGDGAKRR
                     CGMINHSESR"
     gene            2801..3694
                     /gene="atpB"
                     /locus_tag="PLD05_10040"
     CDS             2801..3694
                     /gene="atpB"
                     /locus_tag="PLD05_10040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOP77823.1"
                     /translation="MGLDIAVAGEHVKDATAFHLPFGIHLDLPLIDLGTIQVPGGTFP
                     FSLFGAGREVIEIPLRIQLTKFMVIELAVAVLMFLIFIPMAWHLRGGRPPRGRLMTFF
                     DAILCYIRNHVARPAIGEHEAGRFLPFLWNVFFFVLFCNLMGLLPWAGSPTGSLSVTG
                     VLALLTFGAVVGAGIQEHGLLGYFKGLVPRMDMKGALGIVLVIMLWFIELAGLCIKHA
                     VLAVRLMANMFAGHLVLAVILSFIAAVASMWMILWFGVTVASVAGQVALMMLELFVAF
                     LQAYIFTFLAALFIGMSVHQH"
     gene            3900..4277
                     /gene="atpE"
                     /locus_tag="PLD05_10045"
     CDS             3900..4277
                     /gene="atpE"
                     /locus_tag="PLD05_10045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOP77824.1"
                     /translation="MNKWLTALVLGLVLASSGLLAKGEEVTGGEAGGAAAQAATETQA
                     VEAKGANRFSIFFGGAFGAGLIILGAAFGIGRIGSSAVESMSRQPEVASNIQTAMIIS
                     AALIEGVTFFALIVCLIALFFTG"
     gene            4412..5047
                     /gene="atpF"
                     /locus_tag="PLD05_10050"
     CDS             4412..5047
                     /gene="atpF"
                     /locus_tag="PLD05_10050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOP77825.1"
                     /translation="MALFRQVLPLVLGGLLICAGPQRIVSCAESHAERGEANVAESVN
                     PLDFKADLALWTAVVFVGVLVVLRKFAWKPLVEALDQRENHVRQEILEAENANQEAKR
                     LLAEYQKRMAAVEGEVRAILERARKDAQTMSEELIAKARQEAEAEYQRKLAEIEQAAD
                     QALKELTQRSATLAVELAGKIISARLDPAAHQRIIEQALGSIAELKPPKLN"
     gene            5093..5749
                     /gene="atpH"
                     /locus_tag="PLD05_10055"
     CDS             5093..5749
                     /gene="atpH"
                     /locus_tag="PLD05_10055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOP77826.1"
                     /translation="MTSQNFDAQDSRFAAEFAGDVTVERIAAVYAEAFLNVTEKSGET
                     ESAIAELDAIVDEVLKPHPRFAEILGSNIISHEEKVGIIDRVFKGRVSQLVLNFLKVL
                     SRRGRLDIFLPIVHAIHEEFDRRRGIVPVRVVTAAPLDEKLSEQLATRLRGLIQGEPR
                     VSYRVDPRVIGGIVIEIGDKVIDASVSAQLERLKKQIIDRSAHEIQSRRDRLSHSTGN
                     "
     gene            5700..7235
                     /gene="atpA"
                     /locus_tag="PLD05_10060"
     CDS             5700..7235
                     /gene="atpA"
                     /locus_tag="PLD05_10060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOP77827.1"
                     /translation="MRFKADEIASVIQQEIEQYEARIDVREVGRVLEVGDGIARVYGL
                     SGVMAGEMVEFPNGVRGLAFNLEENSVGVIILGDFLGIQEGDEVRSTGELLSVPVGDA
                     VLGRVIDPLGNPLDGRGPIVTTERRPVESPAPGVAHRQPVKEPLQTGIKAIDSMTPIG
                     RGQRELIIGDRKTGKTAICIDTIINQKGTGVKCFYVAIGQKESSVAQIIDVLRRYGAM
                     DYTTVIVAGASDPAPLQYVAPYAGCAMAEYFMYKGEHALVVYDDLTRQAQAYRQLSLL
                     MRRPPGREAYPGDIFYCHSRLLERAAKLSDELGGGSLTALPIVETLEGEVSAYIPTNV
                     ISITDGQIYLQPDLFFAGQRPAMNVGISVSRVGGKAQIPAMKKVAGGLRLDLAAFREL
                     EAFAQLGTELDPATQARLDRGYRMTELLKQPQYQPMHVTDQVLVIYAGTKGYLDPIPV
                     TQVRNWEKEFLDFVHQNHRALWQHITDTKQLDSETEQKLVAVLEEFNQQYLAKTKEAA
                     ATA"
     gene            7345..8226
                     /gene="atpG"
                     /locus_tag="PLD05_10065"
     CDS             7345..8226
                     /gene="atpG"
                     /locus_tag="PLD05_10065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOP77828.1"
                     /translation="MAKARALERRRRSVRNIRKITRTMELISTARFKRAMDRAMAASA
                     YTQRLTNLLRHLVEAGTEIRHPLMQRHAETRRVLVLVLTSNRGLCGGFNSGVLRLALS
                     HLRSLRTKVEQTRIEVSGKRGISAFKYRRIPVDERYTHFDARPQFEDVERLAVRYWDA
                     FSSGDVDQVDVVYTKFESASRQYPVVETLLPLSSLDMEEENGLLHAVEYEFLPSPESI
                     LEEIVPTAYKMRLFRCFLESAAAEQIARMVAMKSATENADTMLANLSIAYNRARQTQI
                     TSEILEIVSGAEALEKQ"
     gene            8311..9798
                     /gene="atpD"
                     /locus_tag="PLD05_10070"
     CDS             8311..9798
                     /gene="atpD"
                     /locus_tag="PLD05_10070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOP77829.1"
                     /translation="MNATATSGRPRVDVVREPNYGRITQVIGSTFDAEFPEHRLPAIY
                     NAVKIQEQVKGLSINLTGEVQQHLGGGRVRCVALGSTDGLVRGMKCLDTGGPVKVPVG
                     KGILGRVFNLLGEPIDDRGPVEAEDWWPIHREAPPVTELSTKVEIFETGIKVIDLLTP
                     FVRGGKAGLFGGAGLGKTVILTELIARIATSHGGYSVFAGVGERTREGNDLWLEMQEA
                     KIGTTGRKVIEQTCMVFGQMNEPPGARLRVALSALTMAEYFRDTTGTDVLLFIDNIFR
                     FSQAGSEVSALLGRMPSAVGYQPTLATEMGALQERITSTSRGAITSVQAVYVPADDPT
                     DPAPATAFGQLDAFIYLERSIAEKGIYPAVDPLASSSRLLDPQYVGERHYNIARRVQR
                     ILQRYRELQDIIAILGVEELSEEDKLIVHRARRIERFLSQPFLVAEAFTGRPGEITPL
                     SETIRSFEEIVDGKWDHLPEQAFMYVGSIEQAAEQAERMKAEAKS"
     gene            9847..10251
                     /gene="atpC"
                     /locus_tag="PLD05_10075"
     CDS             9847..10251
                     /gene="atpC"
                     /locus_tag="PLD05_10075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOP77830.1"
                     /translation="MANLECVIVTPEKKVLQEPADAVIVPLFDGELGVFPQHAPLIGR
                     LGAGEVRVRSGDDIKRFYVEGGIVEVFGKTVSIITTRAIKGSEIAPDKIHDELGRLAT
                     ERVVGEESLEKRQREYHRLKAMLRVARRSHRP"
     gene            10331..13213
                     /locus_tag="PLD05_10080"
     CDS             10331..13213
                     /locus_tag="PLD05_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77831.1"
                     /translation="MCQCAASDIPEQHDQSSRPGQRYRLSCLSSLILFGVVLLLTFRR
                     VFFTGCLPLFRDSGNFFFPVWAYIVAQYRQGHLPLWNSYLNFGAPLAAENSCAAFYPG
                     ILILMLPIPFSAAYLVFLIVHLCLAAWGIARLARRCGATPVAASLTTLSYPLSGYVLF
                     QIYNPIFLISAAWLPWAIHALFDIFFERNRLTRQSTDNDHATKGNTIHSWLHFLPPVQ
                     ARSAIRLGVFLALMILGGDPHTAYHCAVLAFATWIVLIFSALGRRSNGERNRQPLARV
                     LRRGLLLIGAALVTLLLAAIQIIPTWTYTRLSERALLKPAASDSGNRLTERQDDKTAK
                     STEQNTLAEIARSRQVSQWQRVIYDFSTPAYRWIEFVWPEFGGRPLPWNTRWIGAIVS
                     EGRWWTPSLYMGLLPFLLAILTMRFWPARRSFSSTGSDARYCESSQRNSPNMPPEAFR
                     IWFSWVTVFSLVAALGWYGPAGVYGELWAWITGHSSNGTVSPPVGGLYWVVTQILPGY
                     GLFRYPSKWLVLTTLGLSVLASLAIDHLDTKYLRRLTRLLILILAWATCFLLVALFAS
                     HLWEYASQYVPQDPVFGFFEPGRARWETVTGLVLLLALLLTWLCLVRWVPIRHLPIVI
                     VLLTGLDLVLHNSWMIFPVHVGKETQMALHSLPPFRSWQKWCDYPREWQTPSCTRIQE
                     LFDWERETSAIRWGMLTGRAPLESYGTLVPGDYFVFLAILHEYMNRQKLKIPPQETLA
                     LLGALTTDAFLESNQQEKTSRPDSPTARESHYCFLTHNVTKFPSELSARLNVWQKTEW
                     VFFPNGRPRGPLDTFVVESIEPCGLAILEQIPPKAAQTQERTTKEDCHVKAFQPGKVV
                     IYASLQEPGIVVLREQFMPGWRVHVVSQDGSTSWDARPLRINRIMMGVALPPGKWQIT
                     WTYYDSGFFLGMALSAVAWLGIALMVVLPYLMRISRRLLCQRQGSR"
     gene            complement(13237..14268)
                     /locus_tag="PLD05_10085"
     CDS             complement(13237..14268)
                     /locus_tag="PLD05_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOP77832.1"
                     /translation="MASDFRLKERLPYITDRLVETYRTHGSTSHLGHCPLPNYEAVIQ
                     ATEWMKEILFPGYRRRDGLHMGNVTYYVGDLIDKLHDLLTQQIARALRHEANHSADCD
                     PVRLADFEALGQAKTLAFLEQLPELRKILALDVQAAYDGDPACKSLDEVILCYPGFEA
                     VTVYRLAHLLHELQVPLVPRMMTEWAHSRTGIDIHPGAKIGTYFFIDHGTGVVIGETC
                     EIGNHVKLYQGVTLGALSFPTDAEGRLVRGTKRHPTIEDRVVIYANATVLGGRTVVGH
                     DSVIGSSVWLTRSVPPHSTVTLERPNLRIFGERCEDLKPEASLADAPRTSAKATAGGN
                     GAAPGTTGH"
BASE COUNT         3096 a         3903 c         4159 g         3450 t
ORIGIN      
        1 gcgctcatgc ctggggatca ttttctttca acaaaggttc catcactttg gtgttacgct
       61 ccacagcgag aacgattgtc cgttgttcgg ccccatcagg gctttgcccc acaacgggca
      121 acacgtgtcg gcgatcgggt aacggcaatc gcagggaaaa ccggccatcc gggcccacag
      181 gcacgggttc accgcgaatt gtcacctgat aacctgcctg gatgtgccca aaaacgatca
      241 actcggcatc gacatgaaaa ggaaaccgag agcgatgctc cgtttgcccc tccatcccca
      301 tgcccaaccg ttgaataacc gatccgccca agggccgccg cagccggtcc tccaacacct
      361 cacgcagttc acttgtgtcg gtggcttcat cgtatccgcc actgagggca tatacccgct
      421 cgtactcatc cgcgaggatc gcccagtcaa gatcaggccc ggcggctgat gccagcggtg
      481 tcgtcacaat gttacttcgg gtgagacaga agaacttacc ctcggggctc acgtagccga
      541 tatcgacttc aaaagaccgc ggcggatggt caacattgat ataccagtta ttcacacggc
      601 cgtggatttc gatgtcacga agatggattc gctccacatg actggtacca tcacggcgga
      661 tctcccacag ccgcaggacc ggccgcgcca aatgccaatt ctgccccagc gccgcgcggg
      721 cccgttccac cgtttgacga gcgatctccc aataggcctg caaccagtag gggtcgcgga
      781 ccatgagaac caaacggtct ttggtcgccc ccaagctacc ttcgcgggaa ctgttcgcga
      841 ggtcttttgc gtcaatgagt tcgcgccgta attcctcgac ttgtttttgc acttccgggc
      901 taggcggtgc agccttgact tttcgacgag ttccacccga gacttcggac gtggcgagag
      961 aggcactatt tgatcgcgtt cgagacgatt tttggtcaac ggacttcgct gaagaagaag
     1021 ctttcgacga gtctgccggt tgcccgcgtt ttaccttccc attgccacta ctactttgtc
     1081 ccttgatcct gcccccttta ctcgttttac tggttaccga tttctgtttg gcttttcgct
     1141 gcctagcctc cctccttgct acgtttagga gggcttcaat aagctcctgt ttcctcatgg
     1201 agcgccaacc ggaaattcct tttttcttgg ccatctcggc caagtctttc acggtgtgta
     1261 gtgtcaattc cgcggcggtg atcacagact caacctccgg caaaacgcgc aaatacatca
     1321 ggccatcgcc cgatatcaca ttcccttacg ctccgtgtca tcgcatcgcc gctaggtctt
     1381 gacgtctggc cggctggtgg gccaaaacat acgcagccat cgaggacacg agacgctggt
     1441 ctaggaggtc atccgtgccc tcattctcag tcgcaccaca ggggaaggtt ctgcccgaaa
     1501 taaaggtgac tcgatactta gcagacagcg ccggcgatcg ctcgtcccca gggaccgcac
     1561 cgcttgttgt cgctccctgc tatggctatg ctactgaggt ttattgtaca ctgtgcgcct
     1621 atctttcgca accgtggcgc cattgggagt attttcattg ggccagcgat cacccaaaag
     1681 gcctcttgca ttttcgcttc agcgaggttt ttcgcatcat cacaaaagat acatattaat
     1741 tgacgaatag gagcttaata gagttgtctt aatgtaccgg gctaacccac tgaggtgggt
     1801 tatttgcgca tctaccgaac gattggcgcg agcaatatag ctgaactggg atatttggag
     1861 agaagaaggc aaaattaatc ggtaggagcg atttcccctg cgtcattccg tcggcagaag
     1921 cgggagagtt cgttgggaca tcgacaaaaa cgttccacaa aggacacgcg ggcaacctgg
     1981 gtgatgggtt accaaataag ctctcagatt atcaccatcg ccttggaact cgtcttaccc
     2041 agcctgatgg gttttttcgc agatcgaaag tggcatacct ttccggcgct tacattggtg
     2101 ggggttctca tcggtttttc ggcggggacg ctacattttg tgcagtttgc caaaggattg
     2161 aaccactctc gcgcgaaggc ggccaaaaac gaacagtcca ggcaattcgg agagcctaag
     2221 gaaccgcccc acgaatagca taatgggtaa cctccatgtc gggtcaccag gtcaagcaaa
     2281 tcaatccgcc ctttttgagc acatcctgtg tggactttgc gcggggtacc gttgtgctag
     2341 ccggggtggt gggggtaatt tcactgattt ttgcggccgt gatttttctt gctctgagga
     2401 acccaagtgg cgaactcttg cggctagtgc ttgcggtagt ggtcagcggc tttggcggta
     2461 tggccggtct ggccgttctt tgtttcagcc gaggtacgac ctgggagctt tgggcacctc
     2521 tcgcggccac gcctgttcgg accgcggcta cactgctatc tctggcgctt ctgctcgtcc
     2581 tgccagcagg actagtcacc accagttttc tgctgtatct tatagtgttt tacatctcga
     2641 ttctgggatg cgagacgatt ctagcgctgc agcagctcag aaagaacact gcttcgatag
     2701 gctcggccgt acgtggcgac ggtgcgaaac gccgatgtgg aatgatcaac cattcggagt
     2761 cccgctagcg aggtaagctc taagtggagg ccggcgaaag atgggactgg atatcgcggt
     2821 cgctggtgaa cacgtcaagg atgcgacggc ttttcatctg ccctttggca tccacttgga
     2881 cttgccgttg atcgacttgg ggacaatcca agttcctggt ggtacatttc ccttcagcct
     2941 tttcggggca ggtcgcgaag tcatcgaaat tccgctacgg attcaactca cgaagttcat
     3001 ggtcatcgag ttggctgtgg cggttctcat gtttctgatc tttattccga tggcatggca
     3061 cctgcggggg ggacgaccgc cgcgcgggcg actgatgact ttttttgacg caatcctttg
     3121 ttatatacgc aatcatgtgg cgaggcccgc cattggcgag cacgaggccg gccgttttct
     3181 gccatttttg tggaatgttt tcttctttgt gttgttctgc aacctcatgg gcctgttgcc
     3241 ttgggcgggt tcacccaccg gatcgctgag cgtcacaggt gtcttggccc tgttgacgtt
     3301 cggagccgtc gtcggtgcgg gaatacaaga gcacggtctg ctgggctact tcaagggcct
     3361 ggttcctcgc atggatatga aaggggcact aggcatcgtc ctcgtaatca tgttatggtt
     3421 catcgaactg gcgggactgt gcattaagca cgccgtgttg gccgtgcgtc ttatggccaa
     3481 tatgtttgca gggcatttgg tgctggcggt catcctgagt ttcattgcgg ccgtcgccag
     3541 catgtggatg attttgtggt tcggcgtgac ggttgccagc gtagccgggc aggttgcgct
     3601 catgatgctg gaactgtttg tcgcattctt gcaggcatac attttcacct ttttggccgc
     3661 gctatttatt ggcatgtcgg tgcatcaaca ctagccgact caaggccata taaggacaat
     3721 cgtttggtct aaggaagagc cgacaacagc gaggccggat ttgtacaact caacgagcgg
     3781 tagtagcgta gaaacctgtg agacgttaaa cgacgttcat cggagaaaca cggatacggc
     3841 atggccattg tgcgggccag ggaactgaag ggttttgttg gataaaggag atctttcccg
     3901 tgaacaagtg gcttacagcg ctggtgttgg gactggtttt ggcatccagt ggtcttttgg
     3961 ccaagggcga agaggtcact ggtggtgagg cgggaggagc ggctgcgcag gcggccactg
     4021 agactcaggc cgtcgaggca aaaggggcga accggttttc catcttcttc ggcggggcct
     4081 ttggcgccgg tttgatcatc ttgggtgcgg ccttcggcat cggtcggatt ggaagttctg
     4141 ccgtggaatc gatgtcgcgt cagcctgaag tcgccagcaa tattcagacg gcgatgatca
     4201 tttcggcagc gttgatcgaa ggtgtcacgt ttttcgcgtt gatcgtgtgc ttgatcgcac
     4261 tgttctttac gggctgaggt ttagcgcaag actccctggg cgacggagca ccaccctcgc
     4321 ccagggggta ttttcggttt atcggtgcgc gatcgagtgt tctttttttc tcggacgtat
     4381 gactacgtcg ataagacgga gtaagttagc tatggccttg ttccgccagg ttcttccgct
     4441 ggtcctaggg ggtttgttga tctgcgcggg cccgcagagg attgtttcct gcgccgaaag
     4501 tcacgccgag cgtggggagg ccaatgtggc cgagtccgtc aacccgctgg atttcaaggc
     4561 ggatctcgcc ctttggacgg ccgtggtttt tgttggtgtc cttgtggtcc tgcggaagtt
     4621 cgcctggaaa ccgctcgtgg aggctcttga tcagcgggaa aatcacgtgc gacaggaaat
     4681 ccttgaggct gaaaacgcca accaggaggc aaaacgcctt ttggcggaat atcagaagcg
     4741 aatggcagcc gtggaaggtg aagttcgggc aattctcgaa cgggccagaa aggacgccca
     4801 gacaatgagc gaagagttaa tagccaaagc ccggcaagaa gccgaagccg agtatcagcg
     4861 aaagttggcc gaaatcgaac aggcggcgga tcaagcgtta aaagaactga cccagcgcag
     4921 cgcgacgctt gctgtggagc tggctggaaa gattatctcc gcgagactgg accccgcggc
     4981 ccaccagcgc atcattgagc aagcactggg atcgatcgcc gagttgaaac ctccaaaatt
     5041 gaactgaacg agcggtgggc gtagtcccag aacaaagcgg gaattatcgg tgatgacgag
     5101 tcaaaacttt gacgcccagg actctcgatt tgcggcggaa ttcgccggtg atgtgaccgt
     5161 cgagcgcatc gccgcggttt atgccgaggc gttcttgaac gtcaccgaaa agagcgggga
     5221 gaccgaatcg gcgattgccg aactcgacgc aattgtcgat gaagttttga agccgcatcc
     5281 acgttttgca gagattttgg gctcgaacat catttctcac gaggagaagg ttggcattat
     5341 cgaccgcgtc ttcaaaggcc gagtatcaca actggtgttg aactttttga aagttttgtc
     5401 tcggcgcggg cgtcttgata tctttctccc gattgtccac gccatacatg aagagttcga
     5461 tcggcgacgc ggcatcgttc cggtccgcgt cgtgacggcc gcaccgctcg atgaaaaact
     5521 ctcagaacaa ttggcaacgc gtctccgcgg tttgatccaa ggagaacccc gggtgtcgta
     5581 ccgggttgat cctcgtgtga ttggcgggat cgttatcgaa attggagata aggttatcga
     5641 tgcatctgtg tcggcccaac tggaaaggct gaagaagcaa attatcgaca ggagtgccca
     5701 tgagattcaa agccgacgag atcgcctcag tcattcaaca ggaaattgaa cagtacgagg
     5761 ccaggatcga cgttcgtgag gtcggaaggg ttctcgaagt aggggacggt attgcccgag
     5821 tttacggctt gtccggcgtg atggccggcg aaatggtcga gttccccaac ggtgtccgcg
     5881 gcctggcctt caatctggaa gagaattccg ttggggtcat tattttggga gactttttgg
     5941 gaatccaaga gggcgacgaa gtaaggagca ccggggaact actgagcgtc ccggtgggcg
     6001 acgccgtgtt gggacgggtc atcgaccccc ttgggaatcc gcttgatggt cgcggcccca
     6061 ttgttacgac agagcgacgc ccagtggaaa gcccggcacc cggtgtggcc catcggcagc
     6121 ccgtcaagga accgctccag acgggcatca aggctatcga ttccatgaca cccattggtc
     6181 ggggccaacg ggagctgatc atcggcgacc gtaagacggg aaaaacggcc atttgtattg
     6241 acacgatcat taaccagaag ggaacgggtg tcaagtgttt ttatgtggcc atcggccaga
     6301 aggaatcgag cgtagcgcag attatcgatg tgctccgtcg ctacggtgcg atggattaca
     6361 ccaccgtgat tgtggccggc gctagtgacc ctgctccact tcaatatgtg gcaccttatg
     6421 ctggatgtgc catggccgag tatttcatgt ataagggcga gcatgccctc gtcgtatatg
     6481 acgacttgac acggcaggcc caggcttatc gacagttgtc tttgctcatg cggcggccac
     6541 cgggacgtga agcctatcct ggtgacatct tctattgtca cagccgcctt ctggagcgag
     6601 ccgctaagct gagtgacgaa ctgggcggtg gttcactcac ggccttacct atcgtggaga
     6661 ccctggaggg cgaggtttcc gcctatattc cgacaaacgt catttctatc acggacggcc
     6721 aaatctacct ccagcccgac ctattttttg ccggtcagcg gcccgccatg aacgttggta
     6781 tttcggtctc tcgggtcggt ggcaaggccc agattccggc tatgaagaaa gttgcgggtg
     6841 gtttgcgtct ggacttggcg gcattccgcg agctggaagc tttcgcccaa ctgggaacag
     6901 aactcgatcc cgcaacacaa gcccgccttg atcgtggata tcggatgacg gagcttttga
     6961 agcaaccaca ataccagccc atgcacgtca cagaccaggt gctggtcatt tatgcgggca
     7021 caaaaggata tctggatcct atcccggtaa cgcaagttcg aaattgggaa aaggaatttt
     7081 tggacttcgt acaccaaaat catcgggccc tttggcagca cattaccgat accaaacaac
     7141 tcgattcgga aacggagcaa aaactggtgg ctgtcctgga ggagtttaac cagcagtatt
     7201 tggccaagac aaaggaggca gcggcaaccg catagtgtgc ttgcccgaaa gtttctcgac
     7261 acaaaagatg atgctgtggt gagggttggc gacaagtgag tttggggggc ccggggaacg
     7321 tgatatcaga atagatacgc aggcatggca aaggcaagag cattagagcg aaggcgtcgt
     7381 tcggtccgga atatccgcaa gattacgcgg acgatggagc tgatttccac cgcgcgattc
     7441 aagcgggcga tggatcgcgc catggccgcc tcggcctata cgcaacgact gaccaacctg
     7501 ctgcggcatc ttgtagaggc ggggaccgag attcgccatc cactcatgca gcgacatgcc
     7561 gagacccgtc gcgtgctcgt cttggtgttg acctccaatc gtggcctgtg cggtgggttt
     7621 aactccggtg tcctgcggct ggccctatcc cacctgcggt cgctccggac caaggtcgag
     7681 caaacacgga ttgaggtttc aggcaaacgg ggcatttccg cgtttaaata ccggcgcatt
     7741 ccggtcgatg agcgctacac gcattttgat gcccgtcccc aatttgagga cgtagaaaga
     7801 ctcgcggtgc ggtactggga tgcgttttcg agcggggacg ttgaccaagt ggatgttgtt
     7861 tacacgaaat tcgaatcagc cagccgacaa tatcctgttg tcgaaacttt gttgccttta
     7921 agcagtctcg atatggaaga agagaatggg ctgcttcacg ccgtggaata tgaattttta
     7981 ccttctccgg agagcatttt ggaagagatc gtccccacgg cgtacaaaat gcggttgttc
     8041 cgatgctttc ttgagtctgc tgcggcagaa cagattgccc gcatggtcgc catgaagtcg
     8101 gccacagaaa atgctgacac catgctggcc aatctcagta ttgcgtataa ccgagcccga
     8161 caaacacaaa tcacatcaga gattctggag atcgtcagtg gcgcggaagc tttggagaaa
     8221 caataagtgg tgcgcctcgt tccgcgatcg tgaagcgaca gcccgatcca ttcgatcaat
     8281 caagtgactc cttgttgagg taagaccatg atgaatgcaa cggcaacatc aggacgtccg
     8341 agagtggatg tcgttcggga gcccaactat gggcgaatca cccaggtgat tggctcaacg
     8401 tttgacgccg agtttcccga acatcgtttg ccggcgatct acaatgccgt caagattcag
     8461 gaacaggtta aggggctttc catcaatttg acgggcgaag tgcagcaaca cctgggcggc
     8521 ggtcgtgtgc ggtgcgtcgc cttaggaagc acggacggac tggtgcgggg gatgaaatgc
     8581 cttgacacgg gtgggcccgt caaagtgccg gttggtaaag ggatcctcgg ccgtgtgttc
     8641 aacctgttgg gcgaacccat tgacgatcgt gggccggtgg aggctgagga ctggtggccc
     8701 attcaccgag aagccccgcc ggtgactgag ttgtccacca aagtagagat tttcgaaacc
     8761 ggcatcaaag ttattgattt gctaacaccc tttgtgcggg gcgggaaagc cgggctcttt
     8821 ggtggtgccg gtctggggaa gactgtcatt ctgactgaac tgatcgcaag aattgctaca
     8881 tcgcacgggg gatactccgt gtttgccggt gtcggcgaaa gaacgcgtga aggaaatgac
     8941 ctctggctgg aaatgcagga ggccaagatc gggacgactg gccggaaagt gatcgaacag
     9001 acgtgcatgg tctttggtca aatgaacgaa ccacctgggg cacgtctgcg ggtagctctg
     9061 tcggcgctga caatggccga gtatttccgc gatacaacgg gcaccgacgt gttgctgttt
     9121 attgacaaca ttttccgttt ctcgcaggca ggaagtgagg tctcggcgtt actcgggcga
     9181 atgccctcgg ccgtgggtta tcaaccgacc ctcgctaccg aaatgggcgc tttgcaggaa
     9241 cgtatcacct ccacctcccg tggggctatc acctcggtcc aggcggtgta cgtcccagca
     9301 gacgacccca cagatcctgc cccggccacg gcattcggtc agttggatgc cttcatctat
     9361 ttggaacggt cgattgcgga aaagggtatc tatccggcgg tggatcccct ggcatcgtcg
     9421 agccggctgc tcgatccgca atacgtgggg gaacgccatt ataatatcgc ccggcgtgtc
     9481 caaaggattc tccaacgata tcgggagttg caggacatca ttgccatctt gggtgtggaa
     9541 gaattgagcg aggaagataa gctcattgtt caccgtgcac gccgcatcga acgattcctc
     9601 tcccagccgt tcctggtggc cgaggctttc accggtcggc cgggcgaaat caccccactg
     9661 tcagagacca ttcgcagctt cgaggagatc gtggatggta agtgggacca cctgcccgaa
     9721 caagcattta tgtacgtagg ttcgattgaa caagcggcgg aacaagcgga gcgaatgaag
     9781 gccgaagcca agtcctaagt cccaaatgat tgtcgaattt agtcaagccg tacgataggc
     9841 tcgaccatgg ccaatcttga atgcgttatt gtgacgcccg aaaaaaaggt tcttcaggaa
     9901 cctgctgatg ccgtcattgt gccgcttttt gatggcgagc ttggcgtttt cccgcaacac
     9961 gcccctttga tcggaaggct tggtgcggga gaggtccgtg tccgctctgg tgatgatatc
    10021 aaacggttct acgtggaagg cggcattgtg gaagtctttg ggaaaactgt ttcgatcatc
    10081 accacgcggg caataaaagg gtcagagatc gcccccgaca agatccacga cgaattgggc
    10141 aggttagcaa ccgaacgggt agttggtgag gaaagcctgg aaaaacgcca acgtgaatac
    10201 catcgcttga aggccatgct ccgggtggca cgacgctcgc acaggcctta atctgcttta
    10261 ggtcgcttta gatgaggcct gtgttcgcca aacgcaagtg cctgccacca atttcgtgga
    10321 tgctgcttcg atgtgtcaat gcgctgcctc cgacatcccc gagcagcatg accagtcgtc
    10381 gcgccctggc cagcgttata gactttcttg tcttagtagt cttatcctct ttggcgtcgt
    10441 cctgctactt acttttagga gggtgttttt cacgggttgc cttccgctct ttagggatag
    10501 cgggaacttt ttctttcccg tttgggcgta tatcgtggcc cagtaccgcc aaggtcatct
    10561 gcccttgtgg aattcctatc ttaattttgg tgctcctcta gcggcggaga acagttgtgc
    10621 cgccttttac cccgggattc tgattctgat gttgccgatc cctttttcgg ccgcttacct
    10681 ggtttttctc atcgtccatc tttgcctagc tgcgtggggc attgcgcggt tggctcggcg
    10741 gtgtggtgcc acccccgtgg cggcttccct tacaacgcta tcatatccgc tttcaggtta
    10801 cgtcctgttc caaatataca acccgatttt tttgattagc gcggcctggc tcccttgggc
    10861 catccatgcc ctgtttgaca tattttttga gaggaacaga ctcacccggc aatcaacgga
    10921 taacgaccac gccacgaaag gaaatacaat ccactcatgg ctccactttt tgcccccggt
    10981 ccaagctcga tcagccattc gattgggtgt ttttctagcc ctcatgatcc tagggggcga
    11041 tccacacacc gcttaccatt gcgccgtgct tgccttcgca acctggattg ttcttatttt
    11101 cagtgcactc ggacgccgtt ccaacgggga aaggaaccgc cagccgttgg caagggtctt
    11161 aaggcgcggc ctcctgctaa tcggcgcggc gttggtcact cttctactcg cggcaatcca
    11221 gattattccg acgtggacct acacccgcct tagcgaacgg gcccttctca agcccgcggc
    11281 tagtgattcg ggaaatcgtc tcacggaacg tcaggacgac aagactgcca aaagcacaga
    11341 gcaaaacaca cttgcagaga tcgcccgtag ccggcaagtg tcccaatggc aacgagttat
    11401 ctacgatttt agcacgccgg cctatcgttg gatcgaattt gtctggccag agttcggtgg
    11461 tcgcccccta ccctggaata cgcgatggat cggagcgatt gtttcggaag gccgctggtg
    11521 gacaccgtct ctgtacatgg gactattgcc ctttttgctc gcgatcctca ccatgcggtt
    11581 ttggccagcg cggagatcat tctcgtcaac aggttcggac gcaagatatt gcgaatcgtc
    11641 acagcgcaat tcgccgaata tgcctcccga ggcgttccgc atttggtttt cctgggtcac
    11701 tgtgttcagc ctggtggctg ccttgggctg gtatggtccc gcgggcgtct atggcgagct
    11761 gtgggcatgg atcacgggtc atagtagtaa cgggacggtt tcgcctcctg tcggtgggct
    11821 gtattgggta gtcactcaga ttttgcctgg gtacgggtta ttccgttacc cgtccaaatg
    11881 gctcgtcctg acaactcttg gtctttccgt cctggcttca cttgcgatcg accatcttga
    11941 cacgaagtac ctgcgacggc tgacgcgtct tttgatactg atccttgcct gggccacgtg
    12001 ttttcttctt gtcgccctct ttgcctccca tttgtgggag tacgcctcgc aatacgttcc
    12061 gcaagatccg gtgtttggtt ttttcgaacc cggaagggca agatgggaga ccgtcacagg
    12121 tttggtcctc ctgctggccc ttctcctcac ctggctgtgt ttggtgaggt gggtgccaat
    12181 ccgtcatttg ccaatcgtca tcgtactcct tacgggactg gacctcgtcc tccacaacag
    12241 ttggatgatc ttccccgtac atgtgggcaa ggaaacgcaa atggcgttgc attctcttcc
    12301 gccatttcga tcttggcaga aatggtgtga ctacccacgg gagtggcaaa ccccgtcttg
    12361 tacccgcatt caggagcttt tcgattggga gcgcgaaaca tcggccattc gctggggcat
    12421 gttgacgggg cgcgccccct tggagagtta cggaacactt gttcccggtg attatttcgt
    12481 attcctggcg atccttcatg agtacatgaa tcgccagaag ttgaagatcc cgcctcagga
    12541 aacactcgca cttcttggcg ctttgacaac cgacgccttt ctcgagtcga accaacaaga
    12601 gaaaacaagt cgcccagact cgccgacggc tcgtgaaagt cactattgtt ttctgacaca
    12661 caacgtcacg aagtttcctt ccgaactgtc tgctcgcctg aatgtctggc agaaaacaga
    12721 atgggtcttt tttcccaacg gacgccctcg tggtccttta gacaccttcg tagtggaatc
    12781 cattgaaccg tgtggcttgg cgattctcga gcagatccct cccaaggccg cacaaactca
    12841 agaaagaacg accaaggagg attgtcacgt caaagcattt caacctggca aggtggtcat
    12901 ctacgcctct cttcaagagc cgggaattgt tgtcttacgc gagcagttca tgcccggctg
    12961 gcgggttcac gtggtttcgc aggatggttc cacctcgtgg gatgcccgtc cattgcggat
    13021 caaccgaatc atgatgggtg ttgcccttcc gccgggaaaa tggcaaatca cctggaccta
    13081 ttacgattcg gggtttttct tagggatggc cctctccgcc gtggcttggc tcggaatcgc
    13141 cttgatggta gttctcccct acctcatgcg gataagccgc cgcctcttat gtcaacgcca
    13201 aggaagtcgt tgactaatcc ggacagcccc ggagtattaa tggccggtcg tgccgggcgc
    13261 tgccccgttg ccccccgctg tggccttggc cgacgtgcgc ggagcatcag cgagactggc
    13321 ctcgggcttg aggtcctcac aacgttcccc aaagatccgc aggtttgggc gttccaacgt
    13381 gaccgtcgaa tggggcggca cagaccgggt caaccagaca ctcgacccga tcaccgaatc
    13441 gtgtccaacc acagtgcgtc cccccaagac cgtggcattg gcgtagatca caacgcgatc
    13501 ctcgatggtg ggatgccgtt tagtcccgcg gacgagtcgg ccctccgcat ccgtgggaaa
    13561 actcaaggcc ccaagtgtca cgccttggta aagcttgacg tggtttccaa tctcgcacgt
    13621 ctcgccgatg acaaccccag ttccgtgatc gatgaagaaa taggtaccga tcttcgctcc
    13681 cgggtggata tcaatcccgg tccggctgtg tgcccactct gtcatcatgc ggggcaccaa
    13741 cggaacctga agctcgtgga ggagatgggc aagccggtac actgtgacgg cctcaaatcc
    13801 cggatagcaa aggatgactt cgtccaacga tttgcacgcg gggtcgccat cataggcggc
    13861 ctgcacatca agcgccagaa tcttccgcag ttccggaagt tgctcaagga aggccaacgt
    13921 cttggcctgg cccaaggctt cgaaatcggc cagccggaca ggatcacaat cggcggaatg
    13981 gttggcttcg tgccgaagag cgcgggcaat ctgttgggtt aagaggtcgt gaagtttgtc
    14041 gatcaggtcg ccgacatagt aggtcacgtt gcccatgtga agaccgtcac gacgccgata
    14101 gcctgggaac aggatttctt tcatccactc ggtcgcctgg attacagcct cataattagg
    14161 caaggggcaa tgtcccaggt ggctggttga tccgtgtgtc cgataggtct ctaccagtcg
    14221 gtcggtgata taagggagcc tttccttgag tcgaaagtca gaagccatgt ctcatcctcg
    14281 gtttgaagta cgggtttggt gcatcaacac actgggcaag accgaacact ttttcaaaac
    14341 tgtcaagaaa actacaatgc caaaaaactt gaaagcgagc tcgatgtcgg caagaaggat
    14401 cgtaagatgt actgtccggc cctagaccgg acagcaacac aggcagatta ggcgacagcg
    14461 gaaggatggt gaaggtttat ccatattatg ggaattcctt gccatgccct gaagctaacc
    14521 ctgccgcatt tatgttaacc atgcatagca aaaaagcaag ttcctcgggc aagccttaca
    14581 ttatttcgga cgattgcccc acaaactc
//
LOCUS       DAOSUT010000100        14595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2492_length_14595_cov_9.361004, whole genome shotgun sequence.
ACCESSION   DAOSUT010000100 DAOSUT010000000
VERSION     DAOSUT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_14595_cov_9.361004"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..>3752
                     /locus_tag="PLD05_10090"
     CDS             505..>3752
                     /locus_tag="PLD05_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77833.1"
                     /translation="MVYRRSTRHALVVGLVTVMGLCLGSAWACADETLAQGRLEVFQA
                     ADGATYFAAVMPRIPQESPSGPRHIVVLVNTSAAQTGAVREKSRAALKAFLGRLSDQD
                     RVQIIAVDLKAVALGEGFAAVGSPELDAAVSKLEKRVPLGSCDFGAGLAAATEALKQA
                     PAEGRVLMYFGDGLSRANVLSAETLSQLLTGLVDNKIPVSAYLIGTQVDFGLVASLAT
                     HTGGQLVVDSGDLDAAAVGERLSDAVRATVAFPNGAAWSEQVAEVYPSRMPPLRSDRE
                     TVVVGTLKNPGTVEVEIRFDHASQKFALDGQSHPQNGFLVKLVETARNNAGLVVPVPG
                     ADTLLQLAENAELGVYNLTELARQALEVNDLTSAKRMAEAALAQDPQNQAARKILARV
                     TQVSQQVPAEQPAVPAPAQPAAPQAQAQEPQADLTLVGPQGQAEVPGALAAQVAQDRQ
                     ILAEAVSKDVQASISAARKLMGTNPETAREQLKLKLDEVTRVPDLKPDVREQLLDQLR
                     TAIRQADTRVVELNEQRRRAAQAEAQATERRLIAEGLLLKEQKLSQLMARFNSLMDEG
                     RYELAEVEAAGEAKKLSPDNPVPLLASHMASQVGDFEKAMVLREKRQKNVLDTLYQTE
                     LAHVPIPDEPPILYPDAQVWQELTARRKERYQSMDLGSQNPTERKILSELKKPTELDF
                     VDTPLSEVVNYLKDRHQIEIQLDQKALQDVGVDTNTPITKSLRGISLRSALRLLLREL
                     DLTYVIKDEVLLITTPEQAGNDLVLKVYPVADLVVPPNVMGGAGMGGWGGMGMGMGMG
                     MMGGYGGYGMGGYGMGGMGGYGMGMGGYGMGMGGMWNVPNNNGGFQAFDVKDDLSKSP
                     TGEPRQAAKPSPVLALADQVVSGSVSASQSTDELWNACFDEGVVPAKDVREAVRRLVA
                     AQKFDHVTALIHAALRHNQPQPWMYEGLALALEAQGGSQEEIERALMSAADFATDPMD
                     FLVLGIQLERHGLDQRALKMYRQVAGLMPEWPEPYVAGLRLAQKLNDVEAIQWATVGI
                     LSQVFPEKHQDVWLNALRVAKALLEDLKAQNRTEEAKRYEMALDEAVARDCV"
     assembly_gap    3753..3852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3853..4296
                     /locus_tag="PLD05_10095"
     CDS             <3853..4296
                     /locus_tag="PLD05_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77834.1"
                     /translation="AAAITGHLELRNRILAQQLADNTGNVAQVSSDSMASFLQSRTSG
                     GNGGNRPILPFIPVASGAVGYAPEITVIPEGSMLTAAATISADRRYVRVNPYPTFQGI
                     GEVNIFNFVTGESGVGRGGTGGRGFGGLFGGGGYGGYGGFGGGFF"
     gene            complement(4408..5205)
                     /locus_tag="PLD05_10100"
     CDS             complement(4408..5205)
                     /locus_tag="PLD05_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOP77835.1"
                     /translation="METFFRNEFFEHCNGLVIGMDFLDLTLPQIQANLALDEALLHAA
                     DAGELGEVLRIWEVPSFAVVIGRGSRVAEEVHKETCERLGVPIVRRCSGGCAVVIGPG
                     CLLYSLVFDLNKRSRWRKVDCLHHDVRQKLVDALRQMGVDAVAAGTSDLASISKSTAE
                     CIAKKISGNSVRYARNHVLYHGTLLYAFDLAQMGRLLKQPTRQPEYRHGRSHEAFVTN
                     VPLKRSDLVNMLCRVWDTHRQLPDWPRGRVQALLEERYSRLTWHYER"
     gene            5370..6533
                     /locus_tag="PLD05_10105"
     CDS             5370..6533
                     /locus_tag="PLD05_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP77836.1"
                     /translation="MSYDDRAIVTGDEKEDTVALKPVSAGAEGQGGKPMDPHAEAARD
                     TAQSTAPAVRHLRVDGAKHPPGGLSQNLMWLIFSRIGVPLLMLAGAVLLLQKARLWFR
                     PPLEMPQIIQDIRSDGAARWRAVAQIGAIMADGRYPEVRSDRELAMAMSDAFREELQR
                     PLLSVSDLSVVSRKYLCFLLWQMDDESVILALCEGATWWGISMPQPGSPVRCAALEGL
                     YEMARRLGPDVLRQNPKVLPALRQAASDPIDPVRASAALALGVHGGPGGCETLEGLLN
                     DPSRNVRYSAAIGLSTAGSDKGVLTLKEFFECEEVERLVATGNTESIVAGRTCRLLLL
                     SLESIEVLMNTQPEAQVESLRPAINRFCQSQLPPHVKEVAFRVRRKMQERTGE"
     gene            complement(6578..7534)
                     /locus_tag="PLD05_10110"
     CDS             complement(6578..7534)
                     /locus_tag="PLD05_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOP77837.1"
                     /translation="MRRIVCLSTVCLLMLAVQTLAWAQFKEDTGKGWRLGEPVVHRWQ
                     AGIVVKAVAGACKNMVGTAPLPRDWPEQQVEIVEEDFSPGVSVQYRTVNNLVEQMVVK
                     IPNLPNGQEAKAIVTVEIRRFVQLPPENTSIFKIPTTMPAQVRMFLTPSPKIESHDPK
                     IKEQAKQLITAGDHDWEKVRAIYTFVREHVQYKSGAPLRGAIVALKEGIGDCEDMTSL
                     FVALCRAAGIPARTVWVPGHCYGEFYLVDDEGNGYWFPCQLAGTEAFGEMPDPRPILQ
                     KGDNFVSPVNPRERVRYLAETLTGGGVGGHPQVQFIRKQVAN"
     gene            complement(7617..9674)
                     /locus_tag="PLD05_10115"
     CDS             complement(7617..9674)
                     /locus_tag="PLD05_10115"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOP77838.1"
                     /translation="MNQALSLSRRFVSFLLWGGLLVALSTIPACGDPTGPRAQDRHIA
                     RTVALKLPQAHLTKHPLDQEIADRAFKLFMKSLDPMKLYFYQSDIDRFAAKKSQWVEL
                     LKAGDISPAYETYSVFLQRVDERVETALKILEQPLDFTVDEEIIRDPDLLTYPKTPEE
                     AYERWRKRIKLDLLVLKADKETTGQAAIEKLQKRYTSFRKRVHQTDEDELLETYLSAF
                     CEAYDPHTSYMSPETLENFDITMKLELEGIGAALQSEDGYVVVKKLIPGGAAAKDGRL
                     KIDDKIVGVGQGETGEIVDVVDMNLSDVVKLIRGKRGTVVRLEVLPADGGPKKLITIT
                     RERIELKDSEAKGQVFEAGQKPNGQPYRIGVIDLPSFYMDMSGARLGIPEFRSTTRDV
                     RRILESFNQQGVDAVVLDLRRNGGGSLQEAINLTGLFIESGPVVQVKDSVGRIMVYDD
                     EDTSIVWRGPLIVVISKFSASASEILAGAIQDHRRGIIVGDQSTHGKGTVQSLIDLGQ
                     ELFRLPNAPPLGALKLTIQQFYRPNGESTQNRGVIADIEIPSLTNHLEGIAESDLDYA
                     LPFDQIRPAQYKPYSDVDSSVIEYLKRRSEERVQNSPDFQKVKADIQRYLAQREKKSV
                     PLMEEKFMAQVKELNADKEEEKRLKALTEGNEEGIERDYYLDEVLQIMVDYLQYRVIA
                     QAR"
     gene            complement(9932..11149)
                     /locus_tag="PLD05_10120"
     CDS             complement(9932..11149)
                     /locus_tag="PLD05_10120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOP77839.1"
                     /translation="MGSSNTLNRSDVNPQCGGPNRMALPVVFDVRTVTETGGGPEKTI
                     LLGARWFEDRHWPVYCVYLHPPGDPGFEAIRQKAAELRLSYFLHLDDRGPLDGRLLRR
                     LLALCRQHRPVIWHGHDYKSDALGWLIHRFHPMKLVSTVHGWGVPGRRVWLYHWIHRL
                     CLRGFDRVFCVSAEQYTICRQSGIPPGICEVLENGVDCEFFRPQVNRDEKKEALGIPP
                     NRFVLGYVGRLSPEKNLTLLLDVARKLLAQNCEVHVLLVGDGPERRALSEHAHRIGIA
                     DHITFAGYAADPRPYYAAMDVFLLLSRSEGLPNVLLEALATGLPVICTPVGGIPNVIE
                     SGRNGLVVPLEDVPSPTAAIQQVMTDPKLARRLGEAGRQTVVSRYSFEHRMERLCQSY
                     WLLLGRDEMVETV"
     gene            complement(11162..12988)
                     /locus_tag="PLD05_10125"
     CDS             complement(11162..12988)
                     /locus_tag="PLD05_10125"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOP77840.1"
                     /translation="MSKPTFAEHLQQGVVVFDGAMGTEIYRHHVFTNRCFDELNLSDP
                     KLIEKIHQSYCDAGAEVLTTNTFGANRETLAKYGLAEQTAAINRAGAQIARKVADAAG
                     RPVFVAGSIGPLPNLTYSESDYCSLIWEQVDALMEGGADFIIFETQPSRKAIEMCAAA
                     MQARREVPFVLSAAVYEDGETASGENIQRLLAPLPEGFPSPIAWGFNCGSGPHGLLSA
                     VEQAIRLVNRPLIVQPNAGIPKEVEHRRIYLCSPEYLAEYGKRYVNLGAAAVGGCCGT
                     TPDHIRALCDAVKPLVRARIKPVVVTTEPVALKEPVPFAERSRFAWRLAHKQWVTSVE
                     LVPPRGFDLRDIIDKSKMLHRHGVDAINIPDGPRASSRISPLITADRIQREAMIEVIL
                     HFCCRDRNLIGMQADLLACAACDIRNILFVTGDPPKLGNYPHATGVFDTDSIGMVAVQ
                     KRLNQGVDLGGQSIDPPTAAVIGVGLDPTAIDQKRELDRFQRKVEAGAEFAITQPVFD
                     PDALLRFLDKVQHHGIPIIAGIWPLASLRNATFLKNEVPGVVIPDWVLDKMASVSSRE
                     DQLKMGIEIARYSVERIRDRVAGIQVSAPFGKAAIALEVIGA"
     gene            13253..>14595
                     /locus_tag="PLD05_10130"
     CDS             13253..>14595
                     /locus_tag="PLD05_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOP77841.1"
                     /translation="MPRHQRSQQLHLERASRRDFLTVCGTAAGAGAVTLLSGTVPAVH
                     AGEDHTIRLALIGCGGRGSGAVKDAFSVANGPVELYAMADIFENRLQNSRKILSEMYP
                     QQVNVEGRCFVGFDAYKHAIDCLRPGDVAMLTGYAAFRPVQLEYAVSKGINVFMEKSF
                     ACDPVGVRRVIAAGKAAQEKNIKVAAGLQCRHSVNRHELIRRIKDGELGEIISVRAYR
                     MEAVGPLGPKPADVPELFWQIRNFFRFFWVSGGLFSEMDIHQIDEICWIKDSWPVRAH
                     GVGGRVANSVDRSQNFDSYFAEWTFPDGTQGIDVVRYASNCYNHFATYIHGTKRAAQF
                     SGAVHVGTVRIFKDQRIEEDNILWEAPKEQLTPWQAEWADLFDAIRNDKPHNEAERAA
                     LANLAAIIGRAAVHSGQIVTWEEAMQSQFTFCPNIDQMTAETPPPVLPDERGFYPV"
BASE COUNT         3183 a         3988 c         4188 g         3136 t
ORIGIN      
        1 cgtcaaacgc gttcatacgc cgtcaaatag accgcgtaaa cacttcctct ggcaccggct
       61 acccccagaa atgggggtcg cttccttttt ggcatggagc cgacttttgg ttgggaattt
      121 tcggggccac gcgaaagggg atctgggggg aacttgtggg aaattccaag cgactaatgt
      181 acgtgagtta gaattaattt gaggcgcaag gcttgacttt gccacgcact ctgggggaaa
      241 cgagagccaa ttgcagcaaa ggaaacgacg gtggaggagc tgcgggatca agtctggcga
      301 tcggtaccgg cgatccatcg gttaagattt gccagatttc ctatttctcc caatcgaaag
      361 tttttgcttg gcaaattcag gttccccaag ctacaatggg cttagtctcc acagtgtgag
      421 cgacttctcg tggggagacg ttaagtcaaa agaatcggat gtgatcttca gccggttagt
      481 gaaaggcgtc ggagattgga aaccatggtg tacaggcgat ccacacggca cgcgttggtg
      541 gtcgggctag taacggtgat gggtttgtgc ctcggttcgg cttgggcctg tgctgacgaa
      601 acattggcac aggggcgatt agaggtcttt caagccgctg acggtgccac ctactttgcg
      661 gcggttatgc cgcgtatccc gcaagaaagc ccgagtggtc cgcggcatat cgttgttctt
      721 gtcaacacct cggccgcaca aacgggagct gttcgcgaga agtctcgcgc tgcacttaag
      781 gcatttctcg gtcggctctc ggatcaagac cgtgttcaga ttatcgcggt cgatcttaaa
      841 gcagttgctt tgggggaagg ttttgcggcg gtggggagcc cggaactcga tgcggccgtg
      901 agcaaattgg aaaagcgggt tccgcttggt tcgtgcgatt ttggggccgg gctggcggcc
      961 gccacggaag ctcttaagca agcaccggca gagggtcgcg tcctgatgta ttttggcgat
     1021 ggactgagtc gcgcgaacgt cttatcggca gaaacgcttt cgcagctcct cacggggttg
     1081 gtcgataaca agattcctgt gagcgcctac ctcattggga cgcaagtgga tttcggtctg
     1141 gtggcctcgt tagctacgca taccggagga caacttgtcg tggatagtgg cgatttggac
     1201 gcggcagccg ttggcgagcg tttgagtgat gccgtgcgag cgacggtggc gtttcccaat
     1261 ggcgcagcgt ggtcggagca agtggcagag gtctatcctt cacggatgcc gccacttcgt
     1321 tcggaccggg aaacggtggt cgtgggaact ttaaagaatc ctggcacggt ggaagtcgag
     1381 atccggtttg accatgcctc gcagaagttt gcgctcgatg gccaatctca cccgcaaaat
     1441 gggtttttgg taaagcttgt agaaacggct cgaaacaacg ccggattggt ggtgcccgtt
     1501 ccaggggcgg acacattgct tcaattggcc gaaaatgcgg aactcggcgt ttacaacctt
     1561 acggagctgg cgaggcaggc actggaagtg aatgacctga cgtccgcgaa gcggatggcg
     1621 gaggcggccc tggcgcaaga cccacaaaac caggctgccc gcaagattct cgcccgcgtg
     1681 acgcaagttt cgcagcaagt cccggcggag cagccggcgg taccggcgcc ggcccagccc
     1741 gcggctccgc aagcacaggc gcaagagccg caggccgatc tgaccctggt aggtccgcaa
     1801 gggcaggcgg aggtacccgg tgcgttagcg gctcaggttg cccaagatcg gcagattttg
     1861 gctgaagccg tcagcaagga tgtccaggct tccatcagcg cggcacgaaa gctcatgggg
     1921 accaatccgg agacggctcg cgaacaattg aaacttaagc tggacgaagt cacccgggtt
     1981 cccgacctta agcccgacgt gcgggaacag cttctcgatc agcttcgtac ggcgatccgg
     2041 caggccgata cgcgggtcgt ggagttgaat gaacagcgtc ggcgggcagc tcaggcagaa
     2101 gcccaagcta cggagcgtcg cctgattgcc gaaggtctgc tcctaaagga acagaagttg
     2161 agccaactta tggcccgctt caattccctc atggacgagg gtcgatacga gttggccgag
     2221 gtggaggcgg ccggtgaagc caaaaagctc agcccggata acccggtgcc gcttcttgcg
     2281 agtcacatgg ccagccaagt gggtgatttc gagaaggcca tggtgcttcg cgaaaagcgg
     2341 caaaagaacg ttctcgatac cctctatcaa accgagctag ctcacgttcc cattcctgat
     2401 gaacctccga tcctttatcc cgatgcccag gtgtggcaag agctgacggc tcgaaggaag
     2461 gaacggtatc agagcatgga tttggggagc caaaatccca cggagcggaa aatcttgagt
     2521 gaactgaaga agccgacgga actcgatttc gtggatacgc cgctctcgga agtggtcaat
     2581 tatctgaaag atcggcacca gatcgaaatt caactcgatc agaaagccct tcaggacgtt
     2641 ggtgttgata ccaacacgcc gatcacaaag agtctgcggg gtatttcctt gcgatcggct
     2701 ctgcgtttgc tcctgcggga actcgatttg acatacgtga tcaaggatga agtcctttta
     2761 attaccacgc ccgaacaagc tggtaatgac ctggtgttga aggtctatcc ggttgccgac
     2821 ctggtagtac ctcccaacgt gatgggtggg gcaggcatgg gcggttgggg cggtatgggt
     2881 atgggcatgg gcatgggtat gatgggcggc tacggtggct acggtatggg tggctacggc
     2941 atgggcggta tgggtggcta cggaatgggc atgggtggtt acggcatggg aatgggtggc
     3001 atgtggaacg tgcctaacaa caacggcggg ttccaggcct ttgatgtgaa ggatgatctg
     3061 agcaagtccc ccaccggcga gccacgacag gctgccaagc ctagcccggt tcttgcctta
     3121 gccgatcaag tcgtctcagg aagtgtctcc gcgagccaat ccactgacga gctttggaac
     3181 gcctgtttcg acgagggagt cgttccggca aaagatgttc gggaagccgt tcgtcggttg
     3241 gtggccgccc aaaagtttga ccacgtcacg gcgttgatcc acgccgcatt gcgtcacaat
     3301 cagccgcaac cctggatgta cgaaggcttg gcattggcct tggaagccca ggggggctcg
     3361 caggaagaga tcgagcgagc gctgatgtcc gctgccgatt ttgcaactga tccgatggac
     3421 ttcctggtgt tgggaatcca attggagcgg cacggactcg atcagcgggc tctgaagatg
     3481 taccgtcagg tggctggttt gatgccggaa tggccagaac cttatgtcgc cggcctgcga
     3541 ttggcgcaga agctgaacga cgtcgaagcg atccaatggg caacggtcgg aatcctctcg
     3601 caagtctttc ccgaaaagca tcaggatgtc tggctgaatg ccctgcgggt agccaaggcc
     3661 cttttggaag accttaaggc acaaaaccgc acagaggaag ccaaacgata cgaaatggcc
     3721 ttggacgaag ccgtggcccg ggactgcgtc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nngcggcagc catcacggga cacttggaac tccgaaatcg cattttggcg
     3901 caacagttgg ccgacaatac tggcaatgtg gcccaggtga gctccgactc gatggcgtca
     3961 ttcctccagt cgcggacctc aggggggaat gggggcaata gaccgatctt acccttcatt
     4021 ccagttgcca gcggcgctgt cggatatgct ccggagatca ccgtgatccc cgagggaagc
     4081 atgctcacgg ccgcagccac gatctccgcc gaccggcgat atgttcgcgt caacccctat
     4141 ccgacattcc agggaattgg agaagtcaac atcttcaact ttgtgacggg tgaaagtggc
     4201 gtaggacgag gtggaacggg tggccgaggt ttcggtggct tgttcggcgg tggtggttac
     4261 ggcggttatg gtggttttgg tggcggtttc ttctaattgg gatcgttacc gctttgtttg
     4321 aggctaccaa caagcgctcg ttcgactgtc ggacgagcgt ttcttctttt atggcagcct
     4381 gaagaagcga atccttcccg ggtagggtta ccgctcgtaa tgccaagtca atcggctgta
     4441 tcgctcttca agtaacgctt gcacgcgacc gcgtggccag tcaggcaatt ggcgatgggt
     4501 gtcccagaca cggcaaagca tgttgaccag atcacttcgc tttagcggga cattggttac
     4561 aaaagcctcg tgggaacgac cgtgccggta ttccggttga cgtgtgggtt gcttcaggag
     4621 ccgccccatt tgcgcgaggt cgaaggcgta aagcaacgtc ccatgataca acacgtgatt
     4681 tcgggcataa cgcacactgt taccggaaat tttcttggca atgcattctg ccgtagattt
     4741 agagatgctc gctaaatcgc ttgtgcccgc cgccacagcg tctacaccca tttggcgcaa
     4801 agcgtcaacc aatttctgac gcacgtcatg atgcaagcaa tcgaccttgc gccagcggga
     4861 tcgtttattc aagtcgaaca ccaaggaata taaaagacag ccgggaccga tcacgacggc
     4921 acatcctccg ctacaccgac ggacaatggg gacgcccagt ctctcgcatg tttctttgtg
     4981 gacctcttct gctacccgcg agccacggcc gatcacgacg gcaaaactgg gaacctccca
     5041 aatgcgaagg acctctccca actccccggc atcggcggca tgaaggagtg cctcatccaa
     5101 agccaggttg gcctgaatct gtgggagcgt cagatcgaga aagtccatgc caattactaa
     5161 cccgttgcag tgctcaaaga actcattacg gaaaaacgtt tccatctcgc cggattttgc
     5221 ttgacacagg tcgtcccatg ggccataaaa tacgtcggtt gggatcgcga ggcttttcgc
     5281 cgtgagtcgc gctcttttga atgtgggggt gaaaataccg cccacaatgt gccttgtcaa
     5341 tcaggcaaat ggcgaaagga ggtggggtga tgagttacga cgatcgggcc atcgtgacgg
     5401 gggatgagaa ggaggacact gttgccttga aaccggtctc tgctggcgcg gaaggccaag
     5461 gcggcaagcc catggatccg catgctgaag cagcccgcga tacggcccaa agcacggctc
     5521 ctgccgttcg tcacttgcgt gttgatggag caaaacaccc accaggcggg ttgtcgcaaa
     5581 acctgatgtg gttgattttt tcccgcattg gggtgccact cctcatgctt gctggagcag
     5641 ttttacttct gcaaaaggcg cggttgtggt tcaggccccc tttggagatg cctcagatca
     5701 ttcaggatat tcgctccgat ggagctgccc gatggcgcgc cgtggcccag attggcgcga
     5761 ttatggcgga cggccggtat ccggaggtac gaagcgacag ggagctggcc atggcgatga
     5821 gcgatgcctt tcgagaggag ttgcagcgcc cgctccttag tgtgtccgat ttgtcggtgg
     5881 tctcgcggaa gtatctttgt tttctcctat ggcagatgga tgacgagagt gttattctgg
     5941 cattgtgtga aggtgcgacc tggtggggga tatcgatgcc ccagccggga tcgcccgttc
     6001 ggtgcgcggc tctagaaggt ttgtacgaaa tggcccggcg gcttggcccg gatgtccttc
     6061 ggcagaatcc aaaggtccta ccggcgttgc gtcaagcggc tagtgatccg attgatccgg
     6121 ttcgggcgag tgcggcactc gccttggggg tgcatggggg ccctggggga tgcgaaacat
     6181 tggaagggct cctcaatgat ccctcacgga atgttcgtta cagcgcagcc attgggctga
     6241 gtacggcagg aagtgacaag ggtgtgctga ccttgaaaga gtttttcgag tgcgaagaag
     6301 tggaacgcct ggtggccacg ggcaacacgg aatctatcgt ggctggtcgc acatgccgac
     6361 tgttactctt gagcttggaa tcaatcgaag tacttatgaa tacccaaccc gaagctcaag
     6421 tggagagctt gcgaccggcc atcaaccggt tttgccaaag ccagcttcct cctcacgtca
     6481 aagaggtcgc ctttcgggtg cgaagaaaaa tgcaggagcg caccggggag tgagcgggga
     6541 taacggctct gttgtgatgg ctcggtgaga tcgtccgtca gttcgcaact tgtttacgga
     6601 tgaactgaac ttggggatgg ccgccaacgc cgccgcctgt aagcgtctcg gcgaggtatc
     6661 ggacacgttc gcgtgggttg accggtgaca cgaaattgtc acctttttgc aggatgggac
     6721 gtggatccgg catctcgccg aaggcctccg tcccagcaag ctgacacggg aaccaatacc
     6781 cgttaccctc gtcatcgaca agatagaatt cgccatagca gtggccgggc acccaaaccg
     6841 ttcgtgcagg aatccccgcc gcccggcaaa gggctacgaa tagggacgtc atgtcttcgc
     6901 aatcgccgat tccttctttc agggccacaa tcgcaccccg caacggagca ccacttttgt
     6961 attgcacatg ttcgcggaca aacgtgtaga tggcacgcac cttttcccaa tcatgatcgc
     7021 ccgcagtaat aagttgcttt gcctgttcct tgatcttggg gtcatgcgac tcgatcttgg
     7081 gactcggcgt gagaaacatc cgaacctggg cgggcatcgt cgtggggatc ttgaagatcg
     7141 aggtgttttc cggcggcagt tgcacaaaac ggcgaatctc cacggtcacg atggctttgg
     7201 cctcttgacc gttaggaaga ttcggtatct tgaccaccat ttgctcgaca aggttgttaa
     7261 cggtccgata ctggacactc acccccggcg agaagtcctc ttccacgatt tcgacctgct
     7321 gctccggcca atcccgcggc aggggggcgg taccgaccat gttcttacaa gcccccgcca
     7381 cggctttaac cacgattccc gcttgccaac gatggacaac aggctcgcct aatcgccatc
     7441 ctttgcctgt gtcctctttg aactgcgccc aggcaagggt ctgtaccgcc aacatcagaa
     7501 ggcaaaccgt actcaggcaa acgatgcgtc gcattgtttc cagacaatcc aaatggcttt
     7561 tccccaaagc aacgtggcgg accgctctcg tgaacgatga aggtaagccg gaaagtctag
     7621 cgggcttggg caattacccg atattgcaaa taatccacca taatctggag gacttcatcg
     7681 aggtaataat cccgctcaat cccctcttca ttaccttcgg taagggcctt gagccgcttc
     7741 tcttcctctt tgtcggcatt cagttccttc acctgggcca taaacttttc ttccatgagg
     7801 ggcacactct tcttttcccg ttgggcaaga taccgctgaa tgtcggcctt gaccttctgg
     7861 aagtccggcg aattctgcac gcgctcctcg gagcgtcgtt tcaggtactc gatcaccgac
     7921 gaatcgacat cggaataggg cttgtattgc gccgggcgga tttgatcaaa cggcaaggcg
     7981 taatccaaat cagactcggc aattccttcc aaatggtttg tcaaagacgg aatctcgatg
     8041 tcggcaataa cgccccgatt ttgtgtgctc tcgccgttag gccgatagaa ctgctgaatc
     8101 gtaagtttca gcgcacctag cggtggggca ttgggaagcc ggaacagctc ttgtccgaga
     8161 tcaatgaggc tttgcaccgt tcccttgcca tgcgtgcttt ggtcacccac gataattccc
     8221 cgacggtggt cttgaatggc cccggcgaga atctcgctgg cactggcact gaacttactg
     8281 atgaccacaa tcaagggacc ccgccaaaca atgctggtgt cttcatcgtc atagaccatg
     8341 atccggccga cggaatcttt gacctgcacg accggacccg actcaatgaa taatcccgtc
     8401 agattaatgg cctcttgaag cgacccacca ccattccgcc tcaagtccaa aacaacggcg
     8461 tccacacctt gttgattaaa ggactccaga atccgcctga catctcgcgt cgtgctgcga
     8521 aattcaggga ttcctaaccg cgcccctgac atatccatgt aaaagctggg aagatcaatc
     8581 acgccgatac ggtagggctg tccattcggt ttttggccgg cttcgaagac ctgccctttt
     8641 gcctcgctat ctttcagttc gatcctttcc cgggtaatcg tgatcagctt ctttggtccg
     8701 ccatcagcag gcaacacttc caagcggacg acggttcctc gcttgccgcg aataagcttc
     8761 accacatccg aaagattcat atcgacgaca tcgacgattt cgcccgtctc gccctggccg
     8821 acgcccacga tcttgtcgtc gatctttagc cgtccgtcct tggctgcggc gccaccggga
     8881 atcagtttct tgaccaccac gtaaccatct tcggactgta gcgcagcccc gatgccttcc
     8941 agctcaagct tcatggtgat atcgaagttt tcgagcgttt ccggggacat gtagctggtg
     9001 tgcgggtcat aggcctcgca gaaggccgaa aggtaagttt ccagcaactc atcttcgtcc
     9061 gtttggtgga cacgtttgcg gaagctggtg taacgcttct gaagtttctc aatcgcggcc
     9121 tggcccgtcg tttctttgtc agccttcaaa acgagcaggt cgagcttgat ccgcttgcgc
     9181 caacgttcgt aggcttcctc cggcgttttc ggataagtca acagatcagg atcgcggata
     9241 atctcctcat ccacggtgaa gtccaacggt tgctcgagaa tcttcaaggc tgtctccacc
     9301 cgttcgtcaa cacgttggag gaacaccgag taggtttcat aagcgggact tatatcaccg
     9361 gctttcagaa gctcaaccca ctgcgacttc ttcgcagcga agcgatcgat gtcggactgg
     9421 tagaagtaca gtttcatggg gtcgagcgac ttcatgaaaa gcttgaaggc ccggtcggca
     9481 atctcctgat ccaaaggatg cttcgtgaga tgagcctggg gaagtttgag ggccaccgtc
     9541 cttgcgatgt gccggtcttg ggcacgaggg cccgtggggt caccgcaagc gggaatcgtg
     9601 cttaaagcaa caagcaatcc tccccacaag agaaacgaca caaaacggcg agacaatgac
     9661 agcgcttgat tcatcctttt cacccaaaat gtcaagagaa acacccataa ttgtcaatcg
     9721 ctggttgcta cccggcgact gactttagca cacaatgacc atcatgcgac cgcaaggcca
     9781 cgcaaatccg gccggtccac gataatattt ccgaggcctc tcctacttaa cccattgtat
     9841 ccagttcgag acctgtccgg aaatgaacgc ctgtcggtga atcctaacgg ttttacggaa
     9901 aatgggcaag atcggcgtgt tcgccgccaa ctcatactgt ctcgaccatt tcatctcgcc
     9961 ccaacaaaag ccagtacgat tgacacaggc gttccatacg atgctcgaaa ctgtaacggc
    10021 tgaccacggt ttgccgccct gcctcgccaa gacgtctagc aagtttgggg tcagtcatca
    10081 cctgctggat ggcggccgtc ggactgggga catcttccag gggaacaacg agaccattac
    10141 ggcctgactc gatcacgttg ggaatacccc cgacgggcgt acatatcacg ggtaaccctg
    10201 tggcaagggc ttccagcaac acattaggga ggccttcgct tcggcttaac aggaggaata
    10261 catccattgc ggcatagtag gggcgcggat ccgcagcata gccggcaaac gtgatgtggt
    10321 cggcgattcc aattcgatga gcatgctcgg acaacgcccg gcgctccggg ccatccccca
    10381 cgaggagaac gtgcacctcg cagttttgcg cgaggagctt tcgagccaca tctagtaaca
    10441 gagtcaagtt cttttcggga gataaccgcc cgacgtagcc cagcacaaac cgattgggag
    10501 gaatgccaag agcctctttt ttttcgtcac gattaacttg ggggcggaag aattcgcaat
    10561 ccacgccatt ttccaggacc tcgcaaatcc ccgggggaat gccggactgt cggcaaatgg
    10621 tgtattgttc tgcggacaca caaaatacgc gatcaaagcc gcggagacat agccgatgaa
    10681 tccaatggta aagccacacc cgacgaccgg ggacgcccca accatggacc gtgctcacca
    10741 gtttcatggg gtgaaagcga tggatgagcc agcccagggc gtccgacttg taatcatgtc
    10801 catgccagat caccggtcgg tgttgccgac aaagggccaa aagccgccgc aagagccgcc
    10861 catcgagggg gccccgatca tcgaggtgga gaaaatacga cagccgcaac tcggcggctt
    10921 tttggcgaat tgcctcaaat cccggatcgc ctggcggatg aagatacaca caatacaccg
    10981 gccaatgacg atcctcgaac cagcgggcac caaggaggat cgtcttctcc ggaccgccgc
    11041 ctgtctccgt tacggtacga acatcgaaca ccacgggcag ggccatccga ttgggtccgc
    11101 cgcattgcgg atttacgtca gaccgattaa gggtgttcga cgatcccata tcccttacac
    11161 ttcacgcccc gattacctct aaggcaatgg cggctttgcc aaatggggca ctgacctgga
    11221 tgcccgccac acgatcgcgg atccgttcga ctgagtaccg ggcaatctca attcccatct
    11281 tgagttggtc ttcccggctg gataccgagg ccatcttgtc caaaacccaa tcggggatta
    11341 ccacacccgg tacctcgttc ttgaggaacg ttgcattgcg gagactggct agcggccaga
    11401 taccggcaat gatgggtata ccgtggtgct ggaccttatc gagaaaacgg aggagggcat
    11461 cgggatcgaa cacgggctgc gtgatggcga actcggcccc ggcctctacc ttccgctgga
    11521 aacgatcgag ttcgcgtttc tggtcgatgg ccgtggggtc caaaccaacg ccgatcacgg
    11581 ccgccgttgg cggatcaatg gactgccctc ccaagtccac accctgattg agtcgctttt
    11641 gcaccgccac catgccaatc gaatccgtat caaagacgcc cgtggcgtgc ggatagtttc
    11701 ccagtttggg gggatcaccc gtcacaaaga ggatattgcg aatgtcgcag gcagcgcagg
    11761 ccaacagatc ggcttgcatc ccgatgagat tacggtcacg gcaacaaaaa tggaggatga
    11821 cctcgatcat ggcctcccgc tgaatgcggt cagccgtgat aaggggcgaa atccgtgagc
    11881 tagcccgggg accatcggga atgttgatag catccacccc gtgtcggtgg agcattttac
    11941 ttttgtcgat aatgtcccgc agatcaaatc cgcgcggcgg caccaactcc acagaggtga
    12001 cccactgctt gtgggcaagc cgccaggcaa aacgcgaccg ctcggcgaaa ggcaccggtt
    12061 ccttgagcgc cacgggttca gtcgttacga ccacgggctt gatccgcgca cggacgagcg
    12121 gtttgacggc atcacacaac gcgcggatgt ggtcaggcgt cgtgccgcaa cagccgccca
    12181 ccgcagcagc tcccagattg acgtaacgtt ttccgtattc agccaaatat tctggggagc
    12241 aaagataaat ccgccggtgc tccacttctt tgggaattcc tgcattgggc tgaacgatga
    12301 gcggtctatt gaccaagcgg atggcttgtt ctaccgcaga aagcagcccg tgaggaccgc
    12361 tgccgcaatt aaacccccag gcaatgggtg aaggaaaacc ttccggtaaa ggtgccaaga
    12421 gccgctggat attttcacct gacgccgttt ctccatcttc gtacacagcg gcggaaagca
    12481 caaagggcac ctcacgccgc gcttgcatgg ccgctgcgca catttcaatg gctttgcggc
    12541 tcggttgcgt ctcgaaaata atgaaatcgg cgccaccctc catgagggca tccacctgtt
    12601 cccagataag agaacaatag tcgctttcgg aatatgtcaa atttggcaag ggaccaatcg
    12661 agccggcgac aaagaccgga cgcccggcgg catcggcgac ctttcgcgca atctgggcac
    12721 cagcccggtt gatagcggca gtctgttctg ccagcccgta ttttgccaac gtttcccgat
    12781 tggccccgaa tgtattcgtt gtcagcactt cggccccggc gtcgcagtac gattggtgga
    12841 tcttctcgat cagttttgga tccgagagat tgagttcgtc gaaacagcga ttggtgaaaa
    12901 catggtgacg gtaaatttcc gtccccatgg caccgtcaaa aacgacaacc ccttgttgca
    12961 agtgttcggc gaatgttggt tttgacatag ctacgagcaa gacgattggg cagaaaactg
    13021 acagtttgtg acacgtccct actgctccca cccccttggg tcgcaaactg tcattgtacc
    13081 tcaaacgaaa aaccggcgga gaggtgtaaa cctaggtcta ttgcactgcg gccctctcgt
    13141 ccttatccta acacacggtt tgccgctcgc catgacaaga ggtgagccgg cacatgaaac
    13201 cctaccccag catttccgca tgtagcggac gccgtagcat aaggagttga tcatgccccg
    13261 ccatcagcga tcgcaacaat tacacttgga acgtgcaagc cgacgggatt ttttgacagt
    13321 ctgtggcaca gccgcgggag ctggggccgt gactctgctt tctggcactg ttcctgcggt
    13381 tcatgcgggc gaggatcaca ctatacgttt ggccctcatc ggttgtggtg gtcgtggatc
    13441 gggagccgtc aaagatgcgt tctcggtggc caacggtccc gtagaacttt atgccatggc
    13501 ggacatcttt gagaaccgcc tgcaaaattc ccgcaagatt ctctcggaaa tgtatcctca
    13561 acaggtgaat gtggaaggcc gctgctttgt gggcttcgat gcgtacaagc atgccatcga
    13621 ctgtctgcgc cctggtgacg tggccatgct caccggctat gcggcctttc gccccgtgca
    13681 gcttgaatat gctgtgtcca aggggatcaa cgtattcatg gagaagtcgt ttgcctgtga
    13741 tcccgtgggg gtgcgacggg tgattgcggc tggtaaggcc gctcaggaaa agaacatcaa
    13801 agtggccgcc ggtttgcaat gccggcattc cgtgaatcgc cacgaactga tccgccgaat
    13861 caaagatggc gaattggggg agatcatttc tgtgcgggcg taccgcatgg aagccgttgg
    13921 tcccctcggg ccgaaacccg cagatgtccc cgagcttttc tggcagattc gcaatttctt
    13981 ccgcttcttc tgggtttcag ggggactatt cagcgagatg gacatccacc agattgacga
    14041 aatttgttgg atcaaggact cctggcctgt tcgtgcccac ggtgtaggcg gtcgggttgc
    14101 caacagtgtc gatcgcagcc agaatttcga ttcttacttt gccgagtgga ccttccctga
    14161 cggcacccag gggatcgatg tcgtgcggta cgccagtaac tgctacaacc atttcgctac
    14221 gtatatccac gggacaaagc gggccgcgca attttcgggg gctgtgcacg tgggcacggt
    14281 ccgcatcttc aaagatcagc ggatcgagga ggacaacatc ctctgggagg ccccgaagga
    14341 acaactcacg ccttggcagg cggaatgggc tgatctcttt gacgccatca gaaacgataa
    14401 gccacacaac gaggcggaac gggcggcttt ggcgaatttg gccgccatta ttggccgggc
    14461 ggctgtgcat tccgggcaga ttgtcacctg ggaggaggcc atgcaatcgc agtttacttt
    14521 ctgcccgaat attgaccaaa tgacagccga aacgccgcca cccgttctgc ccgacgaacg
    14581 cggattctat ccggt
//
LOCUS       DAOSUT010000101        14520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2507_length_14520_cov_9.165296, whole genome shotgun sequence.
ACCESSION   DAOSUT010000101 DAOSUT010000000
VERSION     DAOSUT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_14520_cov_9.165296"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLD05_10135"
     CDS             <1..205
                     /locus_tag="PLD05_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP77842.1"
                     /translation="LLQLPGKGPLDFRPLLAALRDIKYSGYSSIFMHPYPRGRPIREN
                     ISLVTSEVLQAKSYLEQCFAELG"
     gene            243..818
                     /locus_tag="PLD05_10140"
     CDS             243..818
                     /locus_tag="PLD05_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOP77843.1"
                     /translation="MEKVLIIVGDATETMDTLYPYYRLIEAGFQPVVAAPEKRRYQMV
                     MHEVKPGWTITKEWEGYTIEADIAFRDVKPEEYLGIFFSGGRAPEYIRYDEDLVRITR
                     HFFETNKPIACVCHGVEIPAYAGCVKGRRMATVPKCRFDLEVCGGIFVDAPCVVDGNL
                     VSGRTYHDHGHYIGAWIKLLEAARDAKSIDR"
     gene            867..2327
                     /locus_tag="PLD05_10145"
     CDS             867..2327
                     /locus_tag="PLD05_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77844.1"
                     /translation="MYRIRALFSAESASANCRSIVRINPYYWRMPLIVSLGALGVFIW
                     GARSAWAEVGSPQRPNIVFILADDLGYGDLPCYGRDDLSTPAIDRLAREGVRFTQAYA
                     NGPECTPTRAAFLTGRYQQRIGGLECAIGIGDVGRYDDAMRLALAGKLGLPTEETTIV
                     SLLQNAGYDTVLFGKWHLGYASKFSPQRHGFRLSRYCLGGGMDYFYYTDTLGIYNLYE
                     NGELLWTKGYFTDLLADWAEAYLSTVGTRPFFLYLAFTAPHSPYQGPDDDPGHPLPGD
                     SPLWDQGRSDPKVYCAMIERMDKAIARVLQSLDRHQLTDNTVVIFASDNGGTRSGRNA
                     PLSGYKGSTQEGGIRVPAIVRWPGHVPPGTTSDQVCITMDFSASIIRLSGAPLPRGCT
                     LDGLDVIALLESGTPPVPRTLFWRARRGDSTWWAVRDSDTKYWRHQKGEKMEEHLFDL
                     ASDPSESHDLLPAQPDRAAVLRKMLARWEEQVKPRR"
     gene            2344..3906
                     /locus_tag="PLD05_10150"
     CDS             2344..3906
                     /locus_tag="PLD05_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP77845.1"
                     /translation="MNQNQPNVLVLNIEGLRAEFLGPYGNDWIDTPHFNRLAVEGFVF
                     DHCLVGGVNSCDQLTALWSGCHPSQALPTRFAIDAGKAQWLVEVFRRAGYFTILTSSS
                     ESVTKHPGASAFDEHRLVAPVKEAAPATSNEHLPVITDPEQLAPVGEPAADWSETRTA
                     AVFSELIQIIETQESPQFIWCQLTTLSAIWESPLELRERFREPGDPPVWPGTNIPRAF
                     FGPGTDPDVYLPFVQAYAAEVYILDLCLGGLLEVLKRNGDKRPWVVVLLSSRGLPLGE
                     HGHVGFAATCFYNELLHVPLIFSFPNGAGQAERSQALVYPHDIMPTLATACGLVDKSD
                     TSLESLPPAGTCETVWIAGRNLLPVIEGSCNFVRNRLFSYSGADVLAIRTPAWLAQIL
                     LSEDGDGPGDLHEQVPERQIPFVDHLKAIVDADDDSEAQNAEDAPQEELLSRVEIAEL
                     RKHVRLFVKPDDRWEINEVADRCPDVAVEFLKMASRFLKVCTPDGDKREPVDFLTLPP
                     LNEVLAKGIGAP"
     gene            3903..5138
                     /locus_tag="PLD05_10155"
     CDS             3903..5138
                     /locus_tag="PLD05_10155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP77846.1"
                     /translation="MTVLSRRNRTHSLLPVPVVFSALVLFVIEAACAQESKVPGIIID
                     HSPKTSGIYIGSPSLAVLPDGTYVASHDEFGPRSTERTRARTRIFTSSDRGEHWQHVA
                     DVDGQFWSSLFVHDGSLYLLGTWSHYGNIVIRQSKDHGKTWTEPLDSNTGLLRSGQFH
                     CAPVPIVIHNGRLWRAFEDTTDPKRWGLPFRARVISAPVNADLLRADSWTLSEPLPGD
                     PAWLNQRFNGFLEGNIVVTPEGGLVNILRVDCPEGGKAALVTVSPDGKRLSFDPEKDF
                     IDFPGGAKKFTIRFDPQSKRYWSLVNAVPLKYRDRKASSVRNTLVLTSSADLRHWTIH
                     CALLHHPDPVYHGFQYPDWLLDGEDIIAVIRTAFDDEAGGAHNAHDANFLTFHRFRGF
                     RSLTWADSVLKPEEFGIDQ"
     gene            complement(5415..5807)
                     /locus_tag="PLD05_10160"
     CDS             complement(5415..5807)
                     /locus_tag="PLD05_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77847.1"
                     /translation="MEFPFRLPLLVEYWSYIVLVWLGFGTLIGLLARMVFPGREPSGP
                     LGTVLIGIMGSVIGPLVVETTCGIQGFHPISPLGFVAALGGTAVILVTYRFFLLIIAD
                     SVPARDDQVEETIAPDHVVGVSHPDYYQ"
     gene            complement(5969..6217)
                     /locus_tag="PLD05_10165"
     CDS             complement(5969..6217)
                     /locus_tag="PLD05_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77848.1"
                     /translation="MNGSARKALLALSLLLCGAMGLLTFTTGCQVDVGGQTLPSPYYM
                     KDDVQYFAPGPEFKLSREAAAIQQAKAQQQAQTATVGR"
     gene            6562..>8144
                     /locus_tag="PLD05_10170"
     CDS             6562..>8144
                     /locus_tag="PLD05_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77849.1"
                     /translation="MMVLLQMRRPDHRPKNHLKWTKSLFLFALLIEIAHLDYPRQLYA
                     QLSSGGQVPPPMYFNAMTFLNEGDYGDALQIFDSCYRSAMRFGTRRFMDSVCYLAMMG
                     ECYYHMGQYDKAYENYTAAVELALSYPTWLQQVTLPPTGPTPLSSAVNRPVPWGQRTR
                     NTALAQLPSTGLYTYAQIIVDPKLQEQGAIVPQEARSINVAEVIRCTALALRRRQELL
                     GPLCLADPLSRAAAGVFSAQYTLPNHWSQCWGDLLGGISAVADGRTNTAIPLLTRATT
                     ASGKFEHQLSAVALIELSKLALARADYTAARTFLLEASCDAFYYGDASLLEEAFRLLS
                     VAHLVSRSQEVDPTVLQALAWAKTKRLSQLQASLDLMVAEQALAAGNVAAATTALDDA
                     HSVISRRQMANGRLGVKLAYLRAAALLRSDAVGQGETALNALLPSIHRCSLWLFQLNR
                     LSRVYGSGQISLRGPITPRTAHELYSLVLRDPTNLDWATDPMECLAVMTAPHLPLYEQ
                     WFAIAWQRKQWEDAIEIADR"
     assembly_gap    8145..8244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8245..9576
                     /locus_tag="PLD05_10175"
     CDS             <8245..9576
                     /locus_tag="PLD05_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOP77850.1"
                     /translation="QESILRSMLVDREAIGVPFPPVVKLKEIQNRLPEGTVLWSFFAS
                     GNNLFVFFIAKDRYDTWQVRGGLLATQRQVGQFLLSLGLQDANREFSLKDLSDTAWQQ
                     PAVNLTNELVAGSSADLTADFPELIIVPDGILWYLPFEALHVKVNGEVLPLIAKHRIR
                     YAVTSSLGVSFQPTSSVPQPRTLIYLGRLNPQEDAAVLEKRAQLLLQSVPGAVVLSTS
                     AVTAPPTLLGKLFDELIVLDDLQGTYTPYAWAPLSWGRTGVTLADWLNLPYRAPQLVI
                     LPGYHTPTESALRKSEGTPTGQEIALALTALQGMGSKTLVVSRWRTGGGTMTSILKEF
                     LQEYPQTSAAAAFQRALLLASSEPLDPNDEPRVQKKAPEKQIVKSHPFLWANMFLVDS
                     GSFAEPAENALPKTEQKEPEEPKMPEEKQDNEKAKDDAKENDAPAAPDVPQ"
     gene            9877..11727
                     /locus_tag="PLD05_10180"
     CDS             9877..11727
                     /locus_tag="PLD05_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77851.1"
                     /translation="MLFGDGPAHVTAFCRVTIEETDMLRACDRRLFQGWSGYLVFICV
                     SAWLASGVQYRFVFGEESALQSAQENQGADGRSDLDRAVELKLSARTFSDLGEVIRLC
                     EEALKKGLSQEDTEFAKQLLAATRIQRGMMIGQTLLGGLTVDRNWPAFRTLALEDLEK
                     GLAAAPDQPEAWLVVSQLQSLPGGDREKALAAASKVVELATDNPRLVARALILRAELV
                     EDKEKALADLDKVVELMPDDPDPLRLRGQTKLQVNKFEEALADFDAALKLEPNHVPTL
                     ESKIDALLELKRFDDALKVCDEIEKLAPLSSEAIFHRARIAAVQKEFEKALELLNKAA
                     ELDPKNPAILMIRAAVYQELKKTEEALKDVNAALALRPGNPALLRLRAALLAGSGKFS
                     EAIADLEQVRKAQSPEDQVLLNLQLGLLYSAQKYYGRAVEHFTKVLEVEPKNVAALRG
                     RGDALLSMGRQAEAIKDYEKALEVDPKDSGVLNNLAWVLATSPDEKLRDGKRALELAK
                     QACELTDYKEAHILSTLAAAYAEIGDMEKAIEWSRKAVELGEGEEKEALQKELDTYLK
                     GEKVRELLQEPPEPASSEESGTSSEQQKPATDDKPAESPPKTDDGASKSP"
     gene            11931..12004
                     /locus_tag="PLD05_10185"
     tRNA            11931..12004
                     /locus_tag="PLD05_10185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11965..11967,aa:Met,seq:cat)
     gene            complement(12122..13024)
                     /gene="ricT"
                     /locus_tag="PLD05_10190"
     CDS             complement(12122..13024)
                     /gene="ricT"
                     /locus_tag="PLD05_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOP77852.1"
                     /translation="MGNKDRQFSTMDTQYIVRFGAMRFLGVFTSTREGDRYKRGDRVI
                     VRTERGLEVGEVLMEASERVLAHLNDAPGGQILRLMTADDEREAARIRRQATQEIEVC
                     ERIVAELGLQMDLVDVEHIFGGERIIVYYLADGRVDFRELVKRLAAEFHTRIEMRQIG
                     VRDEAKLLADYADCGRPVCCNTHLGTIPPVSMKMAKLQKATLDPAKISGRCGRLKCCL
                     RYEFEQYEEWLEKLPPIGAVVQTAQGQGTVVGHELLAQKVLVEGPDGTRKLCSLDKII
                     AVESEPASRGNKNNGEESPEVGPD"
     gene            complement(13087..13737)
                     /locus_tag="PLD05_10195"
     CDS             complement(13087..13737)
                     /locus_tag="PLD05_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77853.1"
                     /translation="MTGWDWFLALVPVGIYLIVIGALNLARRPVVMTGMQDRVLLGLA
                     TIGLIVVGPVELFLPLPVYVAYGPTVWLIAIVLLTLVIGLLILAAPPKLVIFNASVHE
                     VRSLVSETAVELDPESRWAGDCLHMPNLRVQLFLHVSPGWRNATLSAIGNNQDYQGWH
                     RFGQALTKRLAMLEVRPNLRGLYLLLTGSVLLATCMISAFREPTILSQLLSRIISS"
     gene            14059..>14520
                     /locus_tag="PLD05_10200"
     CDS             14059..>14520
                     /locus_tag="PLD05_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77854.1"
                     /translation="MNTLTRRKFLKVPVHLAVGSAGIVLLKNSQSVWSYQANQKLNVA
                     LVGVSGRGAWFVQAIPQIGENVAALCDANERRAAPAFNQFPNVPKFRDFRQMFDKIGN
                     SLDAVVVAVPDHNHAVISNTAICLGKHVYCEKPLTHDVWEARVLRINAAKYK"
BASE COUNT         3310 a         3863 c         3950 g         3297 t
ORIGIN      
        1 gctgttacaa ctgccgggca agggtcccct ggattttagg ccgttgctcg cagccctccg
       61 cgatattaag tattctggct acagttcgat ttttatgcac ccctatccgc ggggaagacc
      121 tatccgagag aatatttcac tggtcactag cgaagttttg caggcgaaaa gctacttaga
      181 gcagtgcttt gctgagcttg gctgaaaggc ttcaaaacag ttaaggtaga gaggaaaccg
      241 ctatggaaaa ggttctcatc attgttggtg acgccaccga aaccatggat acactttatc
      301 catattaccg gctgattgag gccggatttc aacctgttgt cgccgcacca gaaaagcgcc
      361 gttaccaaat ggtgatgcac gaggtaaagc caggatggac gatcaccaag gaatgggagg
      421 gatatacgat cgaggccgac attgcgttcc gagatgtcaa gccggaagag tatttgggaa
      481 ttttcttttc tggcggtcgt gcgccggaat acatccgtta cgatgaagat ttggtgcgga
      541 tcacgcggca tttcttcgag accaataagc ccattgcctg tgtctgtcac ggcgtagaga
      601 tcccggctta tgcggggtgc gtgaaaggcc gacgtatggc gaccgttcct aagtgtcgat
      661 tcgatctgga agtctgtggt gggatttttg tcgatgcgcc gtgtgttgtc gatggaaatc
      721 ttgtcagtgg ccgaacatat catgaccacg gacactacat cggtgcctgg atcaaacttt
      781 tagaagcggc ccgagacgca aaatctattg atcgctaggc tggttagaaa cactttctaa
      841 gaaacacgaa atacggggag acgctcatgt atcggattcg agcacttttc tcggcggaaa
      901 gcgcatccgc aaattgccgt tctattgttc gaatcaatcc ctactattgg cgaatgcctt
      961 tgatcgtttc cttgggtgct ctcggggtct tcatatgggg tgcacgatcg gcatgggccg
     1021 aagtgggaag ccctcaacga cccaacattg tcttcatctt ggccgatgat ctgggttatg
     1081 gggacctgcc atgttatggc cgagatgatc tttccactcc tgccatcgat cggcttgcga
     1141 gagaaggtgt ccgcttcacg caggcttacg ccaacggacc tgagtgcacg cccacaaggg
     1201 cggctttcct gacggggaga tatcagcagc gcatcggcgg acttgagtgt gctattggga
     1261 ttggcgacgt cgggcgatat gacgatgcta tgcgactagc tttggctggg aagctagggt
     1321 tacccactga ggaaacaacc atcgtctccc tattgcaaaa tgccggatat gatacggttt
     1381 tgttcggaaa atggcatctc gggtatgcga gcaagttttc ccctcaacga cacggctttc
     1441 gtttgagtcg ttattgtctt ggcggaggca tggactactt ttattacaca gatacgctag
     1501 ggatctataa cttgtatgag aatggcgagt tgctgtggac caaaggctat ttcactgatc
     1561 ttcttgccga ctgggcagag gcgtatctct ccaccgtagg gacaaggccg ttcttcctgt
     1621 accttgcttt cacagctccc catagtccct atcaagggcc agacgatgat ccaggtcatc
     1681 cattgcctgg ggactcccca ttgtgggatc aaggtcggag tgatcccaag gtttactgtg
     1741 caatgatcga acgaatggac aaggctatag ctcgcgtgct tcaatcactg gatcgtcatc
     1801 aattgacaga taacacagtt gtcattttcg ccagtgacaa tggtggaacc cgcagcggac
     1861 gtaacgcgcc actttcagga tacaagggaa gcacacagga aggtggtatt cgcgtccccg
     1921 caatcgtgcg ttggccagga catgttcctc caggaaccac ctcagatcag gtttgcatca
     1981 caatggattt ttctgcgtcc atcattcgtt taagtggggc cccattgccg cggggttgta
     2041 ctctcgatgg tctcgatgtc attgcccttt tggaatctgg gacaccgccg gtcccgcgca
     2101 ctctcttttg gagggcacga cggggagatt cgacatggtg ggccgtccgg gatagcgaca
     2161 cgaaatattg gcggcatcaa aagggcgaaa agatggaaga gcatcttttt gatctggcca
     2221 gtgacccctc cgagtcacac gatctattgc ctgcacaacc ggaccgagcg gcagtgctac
     2281 ggaagatgct tgcccgatgg gaagagcagg ttaaacctcg tcgctaggga gagcctctcg
     2341 gccatgaatc aaaatcagcc aaatgttctt gttttgaaca ttgaagggct tcgggcggag
     2401 ttcctcggac cttacggaaa tgactggatc gacacgcccc atttcaaccg tttggcggtc
     2461 gaaggctttg tcttcgacca ctgcttggtt ggtggggtca actcatgtga tcaactgacc
     2521 gcactatgga gtggatgtca cccgagccag gcccttccta cgaggtttgc gatcgacgcg
     2581 gggaaagcgc agtggttagt tgaggtattt aggcgagccg ggtatttcac gattctgact
     2641 tcatcgagtg aatcggtgac caagcatcct ggtgccagtg cttttgatga acaccggttg
     2701 gtggcccctg tgaaagaggc ggctcccgcg acgtcaaatg agcatctccc agtcatcacc
     2761 gatccggagc aactagctcc tgttggcgaa ccggccgcag actggtcgga aacacgaacg
     2821 gctgccgtct tttcggaact tatccagatc attgaaaccc aggaatctcc gcaattcatt
     2881 tggtgccaac tgacgacatt gagtgcgatt tgggaatcgc cgctggaact gcgcgaaaga
     2941 tttcgcgaac cgggtgaccc acctgtgtgg ccgggaacaa atatcccacg agcattcttc
     3001 ggaccaggta ccgatcccga cgtgtatttg ccgttcgtgc aagcttatgc ggctgaagtg
     3061 tacatcctcg acctttgctt gggtggactt ctcgaagtcc tgaaacgcaa cggcgacaaa
     3121 agaccgtggg tcgttgtgtt actttcgtct cgcggattgc cgttaggaga gcatgggcac
     3181 gtaggttttg cagcgacatg tttttacaat gagctcctcc atgtgccgct gatcttcagt
     3241 tttccgaacg gcgcgggaca agcagagaga tcacaggcgc tggtttatcc tcacgacatc
     3301 atgccgactt tggccacggc atgcggatta gtggacaagt cagacacatc gcttgagtcg
     3361 ctcccgcccg ccggcacctg tgaaacggtg tggatcgcag ggcgcaattt gctgccggtg
     3421 atcgagggaa gctgcaactt cgttcgtaat cgtctttttt cttatagtgg cgccgatgtg
     3481 cttgccattc gcacgccggc atggctagcg cagattctct tgtctgaaga cggcgacggt
     3541 ccaggcgatt tgcacgagca agtccctgaa cgacaaattc ccttcgtcga tcaccttaag
     3601 gcaattgttg acgctgatga tgattccgag gcacaaaacg cggaagatgc cccccaggag
     3661 gaattgctat cccgtgtaga gatcgctgaa ctgcgaaaac atgtccggct tttcgttaaa
     3721 cccgatgacc gttgggagat taacgaggtt gccgatcgtt gtccggacgt ggccgtcgag
     3781 tttctgaaaa tggcaagtcg cttccttaag gtctgtactc cagatggcga caaacgagaa
     3841 cctgtcgatt ttctgacact tccgccgctt aacgaggtat tagcaaaagg gattggtgcc
     3901 ccatgacggt gttatcaaga cgcaatcgta cccacagttt gcttccagta ccggtcgttt
     3961 tcagcgccct tgtgttgttc gtcattgagg ccgcttgtgc acaagagtcc aaggttccgg
     4021 gaattattat cgatcactca cccaagacgt cggggattta cattggctcg cccagtttag
     4081 cggtcctgcc cgatggaacg tacgtggcaa gtcatgacga gttcggtccc cgttcgacgg
     4141 agcgaacgcg ggcacggaca cggattttta cgtccagcga tcgaggtgaa cactggcagc
     4201 atgtagccga tgtagatggg caattttggt catcgctatt tgtacatgat gggagccttt
     4261 acctacttgg cacatggtcg cactacggca acatcgtcat tcgccagtca aaggatcatg
     4321 gtaaaacatg gactgaacct ttggattcaa acacgggcct gctacgaagc ggacagttcc
     4381 actgcgcacc cgttccaatt gtcattcaca acggccggct ctggcgggcc tttgaagaca
     4441 ccaccgaccc caagcgctgg ggattacctt tccgtgccag ggtcatttcg gccccggtga
     4501 acgcggattt gcttcgggcc gattcgtgga cgctgagcga gccgttgccc ggtgatccgg
     4561 catggctcaa tcagcggttc aacggattcc tcgaagggaa cattgtggtt acaccggagg
     4621 gagggttggt caacatcctg cgtgttgatt gtcctgaagg aggaaaagct gcccttgtga
     4681 cggtgagtcc ggatgggaag cgactgtcgt ttgatcccga aaaagacttc atcgattttc
     4741 ccggcggtgc caaaaaattt acgattcgat ttgatccaca aagcaagagg tattggtcac
     4801 tggtcaatgc ggtaccctta aagtatcgag accgaaaggc ctcgtcggtt cgcaacacgt
     4861 tggttctgac gagttcggcc gacttgcgac actggacgat tcattgcgcg ttgttacatc
     4921 atcccgaccc ggtttatcac ggctttcaat atcccgactg gctgttggac ggcgaggata
     4981 tcatcgcggt gattcgcact gccttcgatg acgaagccgg cggagcccac aatgcccatg
     5041 atgcaaactt cctaaccttc caccgctttc gtggttttcg ttcactgacg tgggcggact
     5101 cggtgctaaa accggaggag ttcggaattg atcagtgaaa gtggtgcaag cagatgatcc
     5161 ttgctcggcg atcgagctgg cgacattggg tcctctgggc ctatcagtag ggtgatcgac
     5221 tgcgtgtccc gccttgtagt tgttgtgagt tcgccaagag taaatcagca ataaggggtc
     5281 tggccaagtt tgcggaactt ggctcgcggt aaccaaggtg taccgcgatt ttttaaagcc
     5341 gatcgtttgg cggacgcgaa agtgcgcgac cgagagaagg ttttgccgac catctgcgac
     5401 cttcggctgg gtccttattg gtaatagtcc ggatgagaaa cgccgacgac gtgatcgggt
     5461 gctatggtct cctcaacctg gtcatcacga gcaggcacag aatcagcaat gatcaataag
     5521 aaaaatcgat aggtaactag gatgaccgcc gtaccaccta gggctgcgac aaaccctagg
     5581 ggactaatgg gatgaaatcc ttggataccg cacgtggtct ctacaactag aggacctatc
     5641 acggatccca taatcccaat gaggacggtg cccaaaggtc cgctcggttc acgaccggga
     5701 aaaaccattc gggctagcaa cccaatcaac gtgccgaacc ccaaccacac caggacgatg
     5761 tagctccaat attccacaag gagaggcaga cgaaagggaa actccatttc cttcgatctc
     5821 cgaaggcctt ttcaacatgc tgcaacctgg catacattca cgaggtcgca gtctcttgcg
     5881 caataggtaa caaagaaaaa cggaaaccga aggtatccct acccccgcgt ttccgcaatt
     5941 gacccgatat gtcgttagag aatccggact accggcccac ggtggctgtc tgagcctgtt
     6001 gttgggcctt tgcctgttga atggccgcag cttcccgtga gagcttaaat tccggcccgg
     6061 gggcaaagta ttgaacgtcg tccttcatgt aataagggct aggcagggtt tgacctccga
     6121 cgtcaacttg gcacccggtt gtgaacgtca aaaggcccat tgcaccacac agaagcaacg
     6181 acaatgccaa aagggctttt cgagctgagc cgttcatttt gaatccccat gttcaagtgg
     6241 agaaaaccac acccgtccaa ttgcgatgca gcgggggccg agtcactcga cagttcggcg
     6301 ccccacatta acgacctcat acggcggctg cctggtgcgt ctccatgacc gttggcaaaa
     6361 ctgtcacaat cgttatcgtc attacattga gcggacttta aacggtcatt ggctgtcccg
     6421 aggagaaaag cgtaagatcg taatatggag gcctcgggga aacctgccga aacggtgcgc
     6481 gccagttatc gtcttcttcg agtaagataa gcgagatacc tgacaattcg cgcggtcccc
     6541 acgtcctgat gaggaaacga catgatggtg ctgctacaaa tgcgtcgccc cgaccatagg
     6601 ccaaagaatc acctgaaatg gacgaagtcc cttttccttt ttgcccttct tatcgagatc
     6661 gctcatcttg actacccacg gcaattgtat gcccagctta gctcgggggg gcaggtccct
     6721 ccaccaatgt acttcaacgc aatgaccttt cttaatgagg gcgactacgg ggacgcgcta
     6781 cagatattcg actcatgtta tcgaagtgcc atgaggtttg gcacgcggcg gtttatggac
     6841 tccgtgtgtt acttggccat gatgggcgag tgctactacc acatgggcca gtatgacaag
     6901 gcgtacgaaa attacaccgc tgccgtggaa ttggcgctca gttatcccac atggcttcag
     6961 caggtaactt tgccccccac aggtcccacg cctctttcca gtgcggtcaa tcgtcccgtc
     7021 ccctggggcc agcgaacaag gaataccgcc ctggcccaac ttccctcaac agggttatat
     7081 acctacgccc aaatcattgt ggacccaaaa cttcaggaac agggcgcgat tgttccgcag
     7141 gaggcacgat cgatcaatgt ggccgaagtg attcgctgca ccgcgctagc ccttcgtcgg
     7201 cggcaagagc tgctgggacc gttatgtctt gcggatcccc taagccgcgc cgcggcaggc
     7261 gtgttttctg cccagtacac gcttcccaac cactggtcgc agtgttgggg cgatctgctc
     7321 ggaggaatca gtgctgtcgc cgacgggcga acgaatacgg ccattcccct tttgacacgt
     7381 gcgaccaccg catcgggaaa gtttgagcat caactgtcag cggtcgcttt gatcgagctg
     7441 agcaagcttg cgctggcccg agccgattac actgccgccc gcacttttct tctggaagca
     7501 agctgcgacg cattttatta cggtgacgca agcctcctcg aagaagcgtt tcgcctcctg
     7561 tcggtggcac atttggtcag tcgatcacaa gaggtggatc ccacagtgct tcaggccctg
     7621 gcttgggcta agaccaaacg tttatcccaa ttgcaggcat cactcgattt gatggttgcc
     7681 gagcaagcct tggcggcagg taatgtggcg gcggcaacca cggcgttgga tgatgcgcat
     7741 tctgtcatct ctcgccgaca aatggctaat ggccgactgg gcgtaaaact ggcctatttg
     7801 cgggcagccg cattattgcg ctcggacgcc gtaggacagg gagaaaccgc gctgaacgcg
     7861 ttactccctt caatccatcg ctgttctttg tggcttttcc agcttaaccg gttatcacgt
     7921 gtttatggct cgggccagat ttctttgcgt ggcccgataa ctccgcgcac ggcccacgaa
     7981 ctctattcgc tggtgttgcg ggatcccacc aatcttgatt gggccaccga ccctatggag
     8041 tgcctcgcgg taatgacggc cccccattta cccctttacg aacaatggtt cgccattgcc
     8101 tggcaacgga aacagtggga agacgcgatc gagattgccg atcgnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnncaggag tcaatcttgc gatcgatgct tgtcgatcgt
     8281 gaagcaattg gtgttccctt tccgcccgtc gtcaaactaa aggaaatcca aaatcgtttg
     8341 cctgagggaa ccgtcctgtg gagttttttt gcttctggaa acaatctgtt cgttttcttt
     8401 atcgccaagg accgttacga cacctggcaa gtgcgcggtg ggttactggc gactcaaaga
     8461 caggttggtc agttcctact ttccttgggg ctgcaagacg caaaccgcga gttttccctc
     8521 aaagatttgt ctgacaccgc ttggcagcaa ccggccgtca atttgaccaa cgagcttgtg
     8581 gccggctcaa gtgccgattt gacggcggac tttcccgaat tgattatcgt cccagatggt
     8641 attctctggt atttgccttt cgaggccctg catgtcaagg taaacggcga agtcctacca
     8701 ctgatcgcca agcaccgcat ccgatatgct gtcacatcgt cgctgggcgt atcgttccaa
     8761 ccgacctcta gtgtcccaca gccgaggaca ttgatctatt tggggcgact caatcctcag
     8821 gaagacgccg ccgtattgga aaagcgagca caactcctgc ttcagagcgt tccgggagcc
     8881 gtggtcttat ccacgagcgc tgtcacggcc ccgcccacat tgcttggcaa attgttcgat
     8941 gagctgattg tgcttgacga tttacaagga acatacacgc catacgcctg ggcgccacta
     9001 tcttggggac gaacgggggt aacccttgcg gactggttga atctgccgta tcgagcaccc
     9061 caattggtta ttctccctgg ttaccataca ccaacggaaa gcgctttgcg gaagagcgaa
     9121 ggaacaccta caggacaaga aattgcgctc gccctgaccg cattgcaggg catggggtca
     9181 aagacgctgg tcgtcagtcg gtggcgaacg ggcggtggaa ccatgacatc gattctcaaa
     9241 gagttcctcc aggagtatcc ccaaacatct gccgcggcag catttcaacg ggcactgctt
     9301 ctggcatcga gcgaacccct cgatcccaac gatgaaccgc gagtgcagaa aaaggctcct
     9361 gaaaagcaga tcgttaagag tcacccattc ttgtgggcaa acatgttctt ggtggattcg
     9421 gggtcattcg ccgaacccgc agagaatgcc ctccccaaaa cagaacaaaa agaacccgag
     9481 gaacccaaga tgccggaaga gaagcaggat aacgagaaag cgaaggacga cgcgaaggaa
     9541 aatgatgctc cggctgcccc agatgttccc cagtaaaatt gattgcaccg tgcatctctg
     9601 aggtcgcccg gacgtattcc gctttcactt ccgataaact gtgtgatcgt aaaccgtgta
     9661 accggagagc aaacccatct tgatccacgt tctgccggta acttttttcg ttttcatggc
     9721 atcttgtggc cggagaggta ccgagtgttc cgaggatttt ctgtctcttg gatcgcgaga
     9781 tacttggcgg aaggggtttc gttttccgtt cctatagcca agacttgcgt cattgcgaca
     9841 taatggggag taagctgtgc gacaaggaga aagtcagtgt tgtttgggga tggtcccgcc
     9901 catgtgacgg cgttttgtcg agtaaccatt gaggagactg atatgttgcg tgcctgtgat
     9961 cggcggttgt ttcaaggttg gtcgggatac cttgttttca tttgcgtctc tgcttggctg
    10021 gcttctggcg tgcagtatcg gttcgttttt ggagaggaat ccgcccttca gtccgctcaa
    10081 gagaatcagg gcgcggatgg acgtagcgat ctcgaccgag ccgttgagct caagctatca
    10141 gcccgcacat tcagtgatct cggggaagtc atccggcttt gtgaagaggc cttgaagaag
    10201 gggctcagcc aggaagacac cgagtttgca aagcaacttt tggcggcaac gcgtatccag
    10261 cggggcatga tgattggcca aaccctcctc ggcgggctga ccgttgaccg gaattggcca
    10321 gcatttcgca cgctagcgct cgaagacctt gaaaaaggcc tggccgccgc tccggatcaa
    10381 ccagaggcct ggttggttgt cagccagctc caaagtttgc ccggcggtga ccgggagaag
    10441 gccctcgccg ctgcctcgaa ggtcgtcgaa ttggctaccg acaatccacg gctcgtggca
    10501 cgagcgttaa ttcttcgggc ggagttggtc gaggataagg agaaggcctt ggccgacctt
    10561 gacaaggtcg ttgagcttat gccggatgac cccgatcccc tgcgattacg aggccaaacc
    10621 aagctgcaag tgaacaagtt cgaggaggcc ctcgccgact tcgatgcagc acttaagctg
    10681 gaaccaaacc acgtcccgac gctcgaatcc aaaattgatg ctcttctcga gcttaagcgg
    10741 ttcgacgacg ccctaaaggt gtgtgatgag atcgaaaagc tggcgccctt gtcgtcggaa
    10801 gcaatttttc accgggcccg aattgccgcg gtccaaaaag agtttgaaaa ggcgttggaa
    10861 cttctcaaca aggcggcgga actggatccc aagaaccccg ccatcctgat gatccgtgct
    10921 gcggtgtatc aggagctcaa gaaaaccgag gaggccctta aagatgtcaa tgcagcttta
    10981 gccctccgac caggaaatcc ggctctcctt cggttacgtg ccgccctttt agcaggcagc
    11041 ggtaagttca gcgaagccat tgccgatttg gaacaggtgc gaaaggccca atcgccggag
    11101 gatcaggtac tcctgaatct ccagttgggt cttctctatt cagcccagaa gtattacgga
    11161 cgggcagtgg agcatttcac caaagtgctc gaggtcgaac ccaagaatgt cgctgcgttg
    11221 cggggacgcg gtgacgcact tttaagcatg ggccggcagg cagaagcgat caaggattac
    11281 gaaaaggctt tggaagtcga tcccaaagac agcggtgtcc tgaacaatct ggcatgggtg
    11341 ctggcgacat cgcccgacga aaaactgcgg gacggtaaac gggccctcga gttggccaaa
    11401 caggcatgcg aattgactga ctacaaggag gcacacattc tcagcacctt ggcggcggct
    11461 tacgctgaaa tcggtgatat ggaaaaggcc atcgaatggt ccaggaaggc tgttgaactt
    11521 ggcgaaggtg aggagaaaga agctcttcaa aaggaactcg atacctacct caaaggtgaa
    11581 aaagtccgcg agttgttgca ggaaccccct gagcctgcaa gcagcgaaga gtcggggact
    11641 tcctctgagc agcaaaagcc ggcaacagac gataaacccg cggaaagccc accgaaaacc
    11701 gatgacggcg catccaaatc accctgattt ctgcgcctcg gctagaccgt ccgtggggtt
    11761 aataccacct gggtgtctgg cagtaaacct gcggaacttc gggataacct agtgataagg
    11821 ctgccccgta tctcggcaag agggtttccg ccgacgcccc tctggacaga ggaaaaagtt
    11881 gcgtcttata attagatgag cggctcgggt gatccgggcg cgtgacctat cgcggggtgg
    11941 agcagtcagg cagctcgcaa ggctcataac cttgaggtcg caggttcaaa tcctgccccc
    12001 gcaacttggg gtatgggttg ccctcgtcag tgtcctttta gcagattttt ctggatttgg
    12061 ggaggtttac ggacagcctg atccagcccg ccatttgaga tttctcacaa gggctgtggc
    12121 tttaatctgg gcccacctcc ggcgactctt cgccattgtt tttattgcca cgactcgccg
    12181 gttcgctttc aaccgcgatg atcttgtcga gtgaacagag ttttctggtg ccgtcgggac
    12241 cttctacaag aactttttgt gcgagaagct catgaccaac caccgtcccc tgcccttggg
    12301 ccgtctgcac gacggcacca atgggaggga gtttttccag ccattcttcg tactgttcga
    12361 attcataccg caagcagcac ttcagccgcc cacatcggcc agaaatcttt gccgggtcca
    12421 gtgtggcctt ttgcaattta gccatcttca tcgaaacggg cgggattgta ccaagatggg
    12481 tgttgcaaca cacgggccga ccgcagtcgg cgtagtctgc gaggagtttg gcctcatctc
    12541 gtacaccgat ttgacgcatt tcaatccggg tgtggaactc cgcggccaga cgcttcacga
    12601 gttctcgaaa atcgacccgg ccatctgcca ggtagtaaac aatgattctc tcgccgccga
    12661 agatgtgttc gacatcgaca aggtccatct ggagccctaa ttcggcgacg atacgctcgc
    12721 aaacctcgat ctcttgcgtc gcctgacggc gaattctcgc ggcttctcgt tcatcatctg
    12781 ccgtcatgag acgaagaatc tgccctccag gggcatcatt tagatgggcc agaacccgct
    12841 cagaggcctc cataaggacc tcgcccacct caagtccccg ctccgtccgc acgatgactc
    12901 ggtcaccgcg cttgtagcga tccccctctc gggtgctcgt gaagacaccg agaaaccgca
    12961 tcgcgccaaa acgaacgatg tattgcgtgt ccatagtgga aaattgccga tccttgtttc
    13021 ccatatcagg gacaacaccg tgtttctgcc ggcatctaaa tacctcacct tgttgcccag
    13081 gaatagtcat gaggaaatga tccgacttag taactgtgac aaaatcgttg gctcacgaaa
    13141 tgcagagatc atgcacgtcg ccaagagcac gctgcctgtc aacaataagt atagaccccg
    13201 tagattgggc ctaacctcca acattgccaa tcgtttggtt aacgcctggc caaagcggtg
    13261 ccatccctgg taatcctgat tgttgccgat ggcgcttaac gtggcatttc gccaacctgg
    13321 ggagacgtgc aaaaagagct gaacccgcaa attgggcatg tgcagacaat caccggccca
    13381 acgggactcc ggatcaagct ccacggcagt ttctgaaacc agcgatcgca cctcgtgaac
    13441 cgatgcattg aaaatcacca gctttggggg agccgcaaga atgagcaacc ctatgacaag
    13501 ggtcaaaagt acgatcgcaa tcagccagac ggtgggacca tacgcaacgt aaacgggcag
    13561 gggcagaaac agttcgaccg gccccaccac aataagccca attgtggcaa gccccaaaag
    13621 tacccgatct tgcatcccgg tcatcactac cggtcgccgc gccaggttta acgctccgat
    13681 gacgatgaga tagatgccga cgggcacaag ggccaaaaac caatcccatc cggtcatttt
    13741 cttcctcttt ccctgcaaac gtggcagcct aacgaacgtg agaacgctag gcgggcgact
    13801 ccctacaacg tcattgtagt ggagccatgc ggacagcgtc gagactcagt aacacctcaa
    13861 aatgccagaa gagggcgaaa gtacgcagga tgtcaccttc ctccagggga ggatgtctcc
    13921 tcccgacgga aataaatccc gttctcccaa ctacggtaag gcttgatctc cagactgttg
    13981 cctgccatcc tccacggtga tacattgttc gatgtccctg tgtccgtcag tcgcaactat
    14041 cgacgaggga ggctcaccgt gaacaccttg acgagaagaa agttccttaa agttcccgtt
    14101 cacttggctg ttggctcggc gggaatcgtg cttttaaaga attcccaaag tgtgtggtcc
    14161 taccaggcca accaaaagct gaatgttgca ctggttgggg tttcgggcag aggggcgtgg
    14221 ttcgttcagg ccatccccca aattggcgag aatgtggccg ctctttgcga tgccaatgaa
    14281 cggcgagcgg ctcccgcatt caatcaattt ccgaatgtgc cgaagtttcg cgactttcgc
    14341 caaatgttcg acaaaattgg caacagcctg gacgccgtcg tggttgccgt tccagatcac
    14401 aatcacgcag tgatttccaa tacggcaata tgtctgggca agcacgtcta ttgcgaaaag
    14461 ccgttgaccc atgacgtatg ggaggcccgg gttctgcgaa ttaacgcggc caaatataaa
//
LOCUS       DAOSUT010000102        14456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2518_length_14456_cov_9.461079, whole genome shotgun sequence.
ACCESSION   DAOSUT010000102 DAOSUT010000000
VERSION     DAOSUT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_14456_cov_9.461079"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1982
                     /locus_tag="PLD05_10205"
     CDS             <1..1982
                     /locus_tag="PLD05_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77855.1"
                     /translation="PLGGSTQVAVQIIRPADAPGAAGKQLTVGSGQTLITWSAAQLGV
                     RVSAPTQVSIGGTTTVRVEVNNPGDLPAENVLLKLTLPSGVSQQSTTPAASVSGATLN
                     WTLGRLNPHEMRTVDIVLQFSQAGTYSLCAEAQAAGNLQAQHCATIAAGAGPAPGPLE
                     LRVQGPDRATVGQDVTYVIEIVNRGTTPLSGLLVRDTFDAGLQHAVGQRAVERDLPGT
                     LQPGETKTIGVLFRVVQPGRWCHTVQVSGPGGIMASQTACLEAQSAGAATTTPETPPS
                     TQPGVGAPSTGLPSTGTTPGAFPGTTTLPSTPQPAVPQPTAPGLPAPPFGSESATSPS
                     SPGGIGFQPPSFGGAPPSTPTTTPPSPSAITLRLSGPTLVRLGESATFTMELVNTGNQ
                     AVSNVQLRCSFDVSWEPLRASAGYLLEGETIVWTLASVEPNQTLRRQIELRAVSASQR
                     ACCRAELVEGSATLASQEACARVEPGAPAPTLRERPSTLSTFGLGNGSQSSAEVAPGG
                     IAPTPEPSSGAKETPSHAEPTRSPPQSSLPVLKTWVIDRYEPITVGKTKTVTVVVENQ
                     GNNPIHGVACQVEVPPEFEVDPDQTLGPAGKATVQGRKVIFDPTDVLNPTETVSFTVG
                     LRAASPGKAHLTAKITCEGMESPMMVERVNTVIGP"
     gene            2029..2379
                     /locus_tag="PLD05_10210"
     CDS             2029..2379
                     /locus_tag="PLD05_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOP77856.1"
                     /translation="MPRFRVLRHDSPRGLHYDLLLETATLLVTFALDREPTPGTTGKA
                     QQLPDHRRQYLEYEGPISGDRGSVAEWDSGLYLIMRRNEQMLDISVEGRMLKGRIVLR
                     LLDASSQEWEFQVM"
     gene            2536..4011
                     /locus_tag="PLD05_10215"
     CDS             2536..4011
                     /locus_tag="PLD05_10215"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOP77857.1"
                     /translation="MEPSASAESHSTASHEGTSSLALRDFLFARTNFERHPAPMGNRV
                     RVFKLARMRELLHRLGSPDRLYPIIHITGTKGKGSTAILAGTMLTASGRRVGIFTSPH
                     LEHVEERLCIDGMPCSRSRFDQLLGEIVPIVRIMDEEAAKRGESGPTYFEMLTAMAFL
                     YFAQEKVDAAVIEVGLGGRLDSTNVCHPQVCVITTVSYDHMDLLGNSLEQIAWEKAGI
                     IKRNLPVVSGVTQPGPRDVIRRVCQKRGCSLYELDRDFGVSYLPPRHLERDTALGNVT
                     FWFQGGEGNGQRTWQEGQLRLLGSHQATNAAVAIMAVRCFQQRLGWRISDQAIQVALR
                     ETIVPARVEVLGRRPVVILDVAHNTASAQALVATVRECFDVRRRYLVFGTTQGKDYRG
                     MLEVLAPHFDHIFLTQYTCNPRALPIEFLIQAAQEVIPTKFSVCPHPLDAWNDAWSRL
                     TAEDLVCITGSFFLAAEMQPILREHGMMSAQSFLTRHSESV"
     gene            4171..4701
                     /locus_tag="PLD05_10220"
     CDS             4171..4701
                     /locus_tag="PLD05_10220"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOP77858.1"
                     /translation="MDKRRGELVMVHNEPILHIVLYQPEIPHNTGAVGRTCVALGAKL
                     WLVRPLGFRITSRHLKRAGLDYWQYLNWEAVDNWAALEAALAGRRFWYLTKTASTLYT
                     EVTYRPGDVLVFGSESQGLPPSLLTAHREACLRIPIRSQVRSLNLSVSVAVAAYEAVR
                     QIGWGGDGSLAPSGPQ"
     gene            complement(4719..6074)
                     /locus_tag="PLD05_10225"
     CDS             complement(4719..6074)
                     /locus_tag="PLD05_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77859.1"
                     /translation="MGPFILAVAFVFVFLAGPLAGQQRTGTPPSDVFGQRRPLLQIDE
                     AKLLAGGLQKITAKYITLYTDCPLTQEILQLGEVFDAAYPQWCEYFGLTPGSRPWRMQ
                     GLLMKDAQRFRQLGVLPEWLPPFTNGYSVGDILWCYDQASDYYRRHLLLHEGTHGFIR
                     AHFGDGVPPWYNEGIAELLGTHFWNGSRLILGYVPRTREEVPLWGRVKIVRDAVRAGQ
                     PLSLAEVLAFGPTAHREVGPYGWCWAATALFDRDARFSPVFRAAVGWVNRPDFQQQFL
                     NHLGNHWRDAQWQWMILLSNLDYGYDFDREASDFARGQPLPPHQVMVRVVADRGWQNS
                     GWYLEAGRTYSLTARGRFVVVQDDPPWESEPQGVTIRYVRGKPLGLLMAAIVPYEPTE
                     PQAELTTVGSFFQPYAVGRHLLLRAARSGTLFLRINDAPAELADNRGDVTVTIEPAAE
                     P"
     gene            complement(6220..6681)
                     /locus_tag="PLD05_10230"
     CDS             complement(6220..6681)
                     /locus_tag="PLD05_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77860.1"
                     /translation="MPAEYSHDNIRFQYPENWRVEEDDLWAGKHSITVYSPEGAFWSV
                     SVYPRDADPQKLAEAAVEAMRQEYDTLEYQSAPLTLAGRSLVGYEMNFFLLDLVCTAQ
                     VHSVRTSQGTYVFFSQGWDRDYEKLAAVFQAMTVSCLNSIKRLSYWDPPDV"
     gene            7015..7848
                     /locus_tag="PLD05_10235"
     CDS             7015..7848
                     /locus_tag="PLD05_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77861.1"
                     /translation="MHREMQATRLLEKSRLMSQQAYFAMEKGDLQEADHLLVQALQLC
                     PQDTEARARYSQLLWRCGRREEALEQMKIALRMAPHDAELHFRIAEQYLELGQLTEAQ
                     AHIQRGLDIARENPNGWVLQGRLALMRNDPDAALRAFHQAAGIRPQDRDILEWIAKAY
                     LARGDTDQALAVRQRIMDTFTPGEESEQALVALAEAYQAVRRYASAAEIYLQAAQKSD
                     HPEPLLLAAAENYYRAGQPGQALGITEQILASNPQNHRARDLQGRLQGVTVASQSPIF
                     R"
     gene            8022..9755
                     /locus_tag="PLD05_10240"
     CDS             8022..9755
                     /locus_tag="PLD05_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOP77862.1"
                     /translation="MSHPSDSRDDHAPGEGVPPPGQDERAADEAAAIQSKQPALSKPA
                     EQPSQPAAKDVPPKRKRPVVLRAPLAKSARLVEEAQIAAMLPTEVATPITPHTPRSRD
                     RYRFFASCLISAVAHAAVLIALSLWMLNLPQGLGPTSLFATWAGQESLEGPEAELVLQ
                     SESQELTPKETELALQQSRINVLESSPLEENNSDEPTAQVADVGDPARPWGVQGATLM
                     QSVERPTGGGLEGRSPEMRQKLAQSGGGTAQSEEAVERGLRWIAAHQDQDGGWSFDLK
                     KGSCRGQCRNPGTEPNTAAATAMALLPFLGAGYTHREGIYEDTLHRGLYYLLRRGRVT
                     ENGLDFQEGTKNGMYAQGLAALVLCEAYAMTKDPALKDPAQQAIRFIESAQDKKGGGW
                     RYRPGEPGDTTVTGWQLMALKSAEMAGLKVDRSVHYGAQRFLDSVASDDGSQYGYLDK
                     TPRRSTTAIGLLCRMYGGWVRGNPPLVRGIGHLASWGPSATDIYYNYYATQVLRHWGG
                     SDWKRWNDAMREYLINMQDTKGHESGSWYFDDEHSRVGGRLYTTAMAVMILEVYYRYM
                     PLYGKQVFPEL"
     gene            complement(9750..10916)
                     /locus_tag="PLD05_10245"
     CDS             complement(9750..10916)
                     /locus_tag="PLD05_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77863.1"
                     /translation="MRVLVVHQTVGPEASQDDKDVLVQAGAVADSLVRLGHEAIIQPA
                     GHSPADLYRLVHRTQPDVVFNLVESFFGKDFFQWWGAGILTELGVPFTGCAEDALFLT
                     GDKLITKSILRATGLPTPEWIVPDDVARDWLACWEEKCLRAQLTEDDAHREDSPIARV
                     FYNLQKRHAGHWHHVTSQDELIAQGDSRPAFLQKGAWSHASAGLIEQLWVIVTRDDLW
                     RLREQLSRRSQQSNEPWFAERYIEGREFNLSLIAGERGPIVFPAAEIDFSAFPPEKLK
                     IVGYSAKWDADSFEYVATPRRFVFPAEDQPLLLQLEALARQCWQVFGLRGYARIDFRV
                     DAAGQPWILEINTNPCLSPDAGFAAAAAEAGWSFDRVTEAILADALRAAGQTAS"
     gene            complement(10918..11940)
                     /locus_tag="PLD05_10250"
     CDS             complement(10918..11940)
                     /locus_tag="PLD05_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOP77864.1"
                     /translation="MKIGFTYDLRSEYLAEGFSEEETAEFDRDDTIQAIEDALRAEGH
                     QVDRIGRIHNLVRRLAAGDRWDLVFNIAEGLYGLGREAQVPALLDAYQIPYTFADPAV
                     LMLSLDKALCKLVVHEAGLPTPAHAVVRCEADVEQVRLPFPLFVKPLAEGTGKGISPQ
                     SKVDTPQALEAACLSLLARFRQPVLVERYLSGREFTVGILGTGKKAKVLGTMEIILRP
                     QAEAHAYSYVNKEFCEELVEYRYPSAESDPEVAEAERVALAAWQAIGGRDAGRVDLRS
                     DETGQPHFLEVNPLAGLHPEHSDLPMIAKAKGLSFRELIRRIIASALERVEASCQTPN
                     CCPAGP"
     gene            complement(11940..12647)
                     /locus_tag="PLD05_10255"
     CDS             complement(11940..12647)
                     /locus_tag="PLD05_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOP77865.1"
                     /translation="MGDEASLCSRCAQRGKTCCQICEIYVTPGDVQRIGTFVGHFDFF
                     EFRKPVDPQYLDQDDDPEWARCVFRPDGSRRVLRRSTSGDCVFLGPVGCRLPWEVRPL
                     VCRLYPVEYTASGLKPDLADGCPVNLLPPGQDLLQALGMTWEKAEVWWRQLYAEIRLE
                     PHLFDTGERDKIAPVEANIFPVRVDGPEADPPAPGGPGGSVLAQTPVGPTASQTGHKS
                     NDLGKPSSYPPAVAKAP"
     gene            complement(12662..13993)
                     /locus_tag="PLD05_10260"
     CDS             complement(12662..13993)
                     /locus_tag="PLD05_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOP77866.1"
                     /translation="MATVDAETASVRAAEEEPPSSGAALNVLSLFETSDNGLLPYPAF
                     SGADSSSLGVPPPVSFRRVRTDIRVRQFRRTFYRDVPDRLWNDWHWQISHRIRTLEEI
                     ERMLVLSPDERAALEQAGAKLPVAITPYYMALISPDDPDQPLRKTVIPRTAEFVRSPG
                     EADDPLGEDKDSPVPGIVHRYPDRVLFLVHDFCSTYCRYCTRSRVVGHGELKSSADRL
                     ALGLDYIRHTPTVRDVLLSGGDPLTLTDEKLDWLLGELRRIPHVEFLRIGTKVPVVLP
                     QRITPQLCRVLRRYHPLWMSIHFTHPDECSPEAYTACGRLADAGIPLGSQTVLLKGIN
                     DNVETMKRLVHELLKMRVKPYYLYQCDPISGSAHFRTPVAKGLEIIEGLRGHTSGYAV
                     PTFVIDAPGGGGKIPLQPNYVVGREGDELILRNYEGAIFRYPDPLEQSLDK"
     gene            complement(14226..>14456)
                     /locus_tag="PLD05_10265"
     CDS             complement(14226..>14456)
                     /locus_tag="PLD05_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77867.1"
                     /translation="LPGEVFADLGLSIKAASPFRQTFVVGLANGLVGYIPNRSAYAEG
                     QYEVVSARCAEGSGEMLVNAALRLLTELHGAE"
BASE COUNT         3089 a         4447 c         4199 g         2721 t
ORIGIN      
        1 agcccttggg gggatcaacc caggtcgcgg tgcagattat ccgaccggcc gatgcccctg
       61 gtgccgccgg gaagcagttg accgtcggaa gtggccagac actcatcacc tggtcggcag
      121 cgcaattggg ggtccgtgtt agcgcgccca ctcaggtgtc tatcgggggg acaaccacgg
      181 tccgcgtgga ggtcaataat ccgggcgacc tgcccgcgga aaacgtactt cttaagctca
      241 cgctgcccag cggggtttct cagcaaagca cgactcccgc cgcctccgtc agcggggcga
      301 ccctcaactg gacgctcggg cgtctcaatc ctcatgagat gcgaacggtg gacatcgttc
      361 tccaattcag tcaagcaggg acctatagcc tctgcgccga ggcacaggcc gcggggaatt
      421 tgcaggccca gcactgtgca accatcgccg ccggggccgg acccgccccg gggcctctcg
      481 aattgcgggt ccaggggccg gaccgcgcta ccgtaggcca ggatgtcacc tacgtcatcg
      541 agatcgtgaa tcgtgggaca acgcccctga gcggcttgct cgtgcgagac acgttcgatg
      601 ccggactgca acacgccgtg ggtcaacggg ccgttgagcg ggacctcccg ggaacgctcc
      661 agccagggga aaccaagacg atcggcgtgc tcttccgcgt ggtccagccg ggcaggtggt
      721 gccacacggt gcaagtcagt gggccgggcg ggatcatggc ctcgcagacc gcctgcctcg
      781 aagcccagag cgcgggagcg gccacgacga cgccggaaac accgccatcc actcagccgg
      841 gcgtcggggc gccttcgacc gggctgccct ccacggggac cacgcccggt gcgtttcccg
      901 gtacaaccac tttacccagc acgccacagc cagccgtgcc gcagcccact gcaccgggtc
      961 tgccggcgcc acctttcggc agcgaatcgg ccacatcgcc atcatcacca ggcgggatcg
     1021 gttttcagcc tccaagtttt ggcggtgcac cgccgtcaac tcccaccacc acacctccgt
     1081 caccttcggc catcacgttg cgattgagtg ggccaacgct ggtccgactg ggcgaatcgg
     1141 caacgtttac catggaactc gtcaacacgg gaaatcaagc tgtcagtaac gtgcaattgc
     1201 ggtgtagttt tgatgtctca tgggaacccc tccgcgccag tgccgggtat ttgttggagg
     1261 gcgagaccat cgtctggacg ctcgccagcg tggaaccgaa ccagaccctg cggcggcaaa
     1321 ttgaactgcg tgccgtgagc gcctcgcagc gagcatgttg ccgggcggag ttggtcgaag
     1381 gatcggccac actcgccagc caggaagctt gtgcccgcgt ggagcctggt gccccagcgc
     1441 ccacacttcg cgaacgcccc tccacgttga gcacgttcgg cctcgggaat ggttcccaat
     1501 catccgcgga agtcgcgccc ggtggaatcg ctccaacacc ggagccgtcg agcggagcaa
     1561 aggagacgcc ttctcacgca gagccgaccc gatcgcctcc ccagtcctcg ctgcccgtgc
     1621 tgaagacgtg ggttattgat cgttacgagc ccatcaccgt gggaaagaca aagacggtca
     1681 cggtcgtcgt agagaatcaa gggaataacc ctatccacgg cgtggcctgc caggtggaag
     1741 ttcccccaga gttcgaagtt gatcccgatc agacgctcgg acctgcggga aaggcgacag
     1801 tccaagggcg aaaggtgatt tttgatccga cggatgtgct gaatcctacg gaaacggtct
     1861 ctttcacagt gggactaagg gctgcctcac ctgggaaggc tcatttgacg gcaaaaatca
     1921 cctgcgaggg aatggaatcg ccaatgatgg tggaaagggt aaacaccgtc atcgggccat
     1981 gaaaggcagt tgcgatcctg tggtacttcc aacgggcgga gggtggccgt gccccgattt
     2041 cgagtcttac gacacgacag tccgcgggga ctgcattatg accttctctt ggaaacggcc
     2101 acactgttgg tgacgttcgc gctggaccgg gagccaactc cggggacaac gggcaaagcg
     2161 cagcagttgc ccgatcatcg ccgacagtat ctggaatacg aaggccctat ctccggggac
     2221 cgggggagcg tggcggaatg ggacagcggg ttgtacctca tcatgcgtcg caacgagcaa
     2281 atgctggaca tttccgttga gggtcgcatg cttaagggac ggattgtcct ccgactcctg
     2341 gatgcctcat cgcaggagtg ggagtttcag gtgatgtgat ggcgccgaag gtgcggcttt
     2401 agcccaaaag tggggttgcg catcttggga caggtttatt tttgtgagtg caacgttggc
     2461 aaaggtggcc gtgggtgcaa caattaagta tggtttttga gaactgtggc cacactccag
     2521 tagcgtaggg cctccatgga accttcagcc tctgccgaaa gccatagcac ggcctcccat
     2581 gaagggactt cttccctcgc cttgcgggac ttcctttttg cgcggaccaa ttttgagcgc
     2641 catccggccc cgatgggcaa tcgtgtccgc gtcttcaaac tcgcccgcat gcgggaactc
     2701 ctccatcggt tgggcagtcc cgatcgactc tatccgatca tccacatcac ggggacgaag
     2761 gggaaaggtt ccacggcgat cctggcaggg accatgttga cggcgtcagg gcggcgagtg
     2821 ggtatcttta cctctcccca cctggagcac gtcgaggaac gattgtgcat cgacggcatg
     2881 ccgtgttcac gctcacgatt tgaccagctt cttggcgaga tcgtccccat tgttcgcatc
     2941 atggacgagg aagcggcaaa acgtggcgaa agtgggccaa cgtactttga aatgctcacc
     3001 gccatggcgt tcctctattt cgcccaagaa aaagtcgatg ccgccgtgat cgaggttggt
     3061 ctgggtggcc gcttggactc gaccaatgtc tgccatccgc aagtctgcgt catcaccacc
     3121 gtcagttatg accacatgga cctgttaggc aattcgctag aacaaattgc ctgggaaaag
     3181 gctgggatca tcaagcgcaa cctgccggtg gtaagcggtg ttacccagcc gggtccgcga
     3241 gacgtcatcc gccgggtctg ccaaaaacga ggctgttcct tgtacgaact tgaccgagat
     3301 ttcggtgtgt cgtatctgcc gccgcggcat ctcgaaaggg acaccgcctt gggaaatgtc
     3361 accttttggt tccaaggagg tgagggcaat ggccaacgga cgtggcagga aggacagtta
     3421 cggcttctgg gcagtcacca agcgaccaat gccgccgtgg ccatcatggc cgtcaggtgt
     3481 ttccagcagc gcctgggatg gcgcatttca gaccaggcca ttcaggtggc cctccgcgaa
     3541 acgattgtcc cggcacgggt cgaggtcctc ggccgacgcc cggtcgtcat tctggatgtc
     3601 gcccacaata cggcctccgc acaggctttg gtcgccacgg tgcgtgagtg ctttgatgtc
     3661 cggcggcgat acctcgtctt tggaaccacc cagggcaagg attaccgcgg gatgttggag
     3721 gtgctggccc cgcacttcga tcacatcttc ctcacgcaat acacttgtaa tccgcgagcc
     3781 ttgccaattg agtttctcat tcaggcggcc caggaagtca tccccaccaa gtttagtgtg
     3841 tgtcctcatc cgctcgatgc ctggaacgat gcctggagca gactcacggc cgaggacctg
     3901 gtctgtatta cgggctcatt tttcctcgcc gccgaaatgc agcccatcct gcgggaacac
     3961 ggcatgatgt cggcccaaag ctttctcacg cgacattcag aaagcgtcta ggaacctatc
     4021 ggaaaattcc ctctccctct gggagagggt tggggtgagg gtaagccatc gttggagtcg
     4081 atcgagaaaa ccagcagcga gtcgagccac cctcaccact ggcccctctc ccgccgaacg
     4141 ggagaggcga agttgtggga taacgtctaa gtggacaagc gtcggggcga gctagtcatg
     4201 gtgcacaacg aaccaatcct gcacatcgtc ctctatcaac cggaaatccc gcataatacc
     4261 ggggcggtcg gtcgaacgtg cgtcgcccta ggggccaaat tgtggctcgt tcgcccactg
     4321 gggtttcgca tcacaagccg ccaccttaaa cgggcagggc tggattactg gcagtatttg
     4381 aactgggaag ctgtggacaa ttgggcggcc ctggaagccg ctctcgccgg gcgacgcttt
     4441 tggtatttga cgaagacggc ctccacgctc tacaccgagg tgacttaccg gcccggcgac
     4501 gtcctagtgt ttggcagcga atcccaaggc ctgcccccta gtttactcac tgcacatcga
     4561 gaggcctgcc tccgcatccc catccgttct caggtccgca gcctcaatct atcggtcagt
     4621 gtggccgtgg ccgcatacga agcggtacgc caaatcggct ggggtgggga cggctctcta
     4681 gctccctcag gaccgcaatg atcgtcgggt gatgcctgtc acggttccgc cgctggctcg
     4741 atggtgaccg tcacatcccc ccggttatcg gccaattcag caggtgcgtc gttgattcga
     4801 aggaaaagtg tcccgctccg cgccgctcta agtaacaagt gacgccccac ggcgtaaggt
     4861 tgaaagaagc tcccgaccgt ggtcaactcc gcctgcggct cggtgggctc ataaggcaca
     4921 atggccgcca tgagaagacc caggggtttc cctcgcacat agcgtatcgt caccccttgg
     4981 ggttcgctct cccagggagg gtcgtcttgg acgacgacga atcggccacg ggcagtgagc
     5041 gagtacgtgc gccccgcctc caaataccag cccgagttct gccaacctcg gtcggcaacc
     5101 acacggacca tgacctgatg aggtgggaga ggctgccccc gggcgaaatc gctggcttcc
     5161 cgatcaaagt cgtagccgta atccagattg cttagcagga tcatccattg ccattgggca
     5221 tctcgccagt gatttcccaa gtgattcaaa aactgctgct ggaaatcggg gcgattgacc
     5281 cagcctacgg cagcccgaaa gaccggagaa aaccgcgcat cccgatcaaa aagggccgtt
     5341 gccgcccaac accaaccgta tggtcccacc tcgcgatggg ccgtcggccc gaaggccagc
     5401 acctcggcca gcgacaacgg ctgacccgcc cgcacagcgt ctcgaacgat cttcacgcgt
     5461 ccccaaaggg gaacttcctc tcgcgtccgc ggcacataac caaggatcaa tcgactgccg
     5521 ttccaaaagt gagtccccaa taactcggca atcccctcgt tgtaccacgg gggcacacca
     5581 tccccaaaat gggcgcggat aaagccgtgg gtcccctcgt gaagaagcaa atggcgccgg
     5641 taataatcgc tcgcctggtc gtaacaccaa aggatgtcgc ccaccgaata cccgttggta
     5701 aaaggcggca accactctgg aaggacaccc aattggcgaa accgctgggc gtctttcatc
     5761 aacaagccct gcattcgcca ggggcggctc cccggcgtga gaccaaagta ttcgcaccac
     5821 tgcggataag cggcatcgaa gacctcgccc agttggagga tttcctgcgt gagggggcaa
     5881 tccgtgtaga gcgtaatgta ctttgccgtg atcttctgca gaccgccggc gagaagcttg
     5941 gcctcatcaa tctgcagcag cgggcgccgc tggccaaaga cgtcactcgg aggtgttccc
     6001 gtgcgctgtt gtcctgccaa aggaccggcc aggaagacaa aaacgaaggc tacggcgagt
     6061 atgaaaggcc ccaacgtcct caggggcata cggcccggtg aaagcgggct gttcttgctc
     6121 gaagctatag cgcatggacg tgtccctttg agtggcaaga caaatccgca gcgtagggag
     6181 tgctgtccct tgctgcctct cggaactcgt gttgccgtgt cagacatcgg gaggatccca
     6241 gtatgacaat cttttgatcg agttgagaca actgacggtc atcgcctgaa acacggcagc
     6301 cagtttctcg taatcccgat cccagccttg gctgaagaaa acatacgttc cttgacttgt
     6361 ccgcacactg tggacctggg ccgtacacac gagatccaag agaaagaagt tcatctcgta
     6421 ccccaccagc gaacgcccgg caagagttag cggggccgac tgatattcaa gcgtgtcgta
     6481 ttcctgacgc atcgcctcca cggcggcctc ggccaatttc tgtggatcgg catcccgcgg
     6541 atagacgctg acactccaga aagccccctc ggggctgtaa actgtgatgg agtgcttccc
     6601 cgcccaaagg tcgtcttctt ccacccgcca gttctcggga tactgaaaac ggatgttgtc
     6661 gtggctatac tcggccggca tgggaacttc ctaacttttt tgcgatagcg ccttgtcact
     6721 caaaaaacag cgtctcttta gatggtgcca tcacgcaact tgtgttttgg tgatctggaa
     6781 tatctacgaa aaatacaccg agaaattgct ctaggaccga acgacatcac tcagtatcat
     6841 gaccccaggc aacgtcctgt ttaatttttt ctcgttccac cgttggaggc gcaaggaaat
     6901 cactcaaaaa cggtgtgggg acgggtcacc atcgcttata tgattgattt taccaaaact
     6961 gcggcaattt gggccgtcct cggcctcgtg gtcggcgggg gtggatgccg tcttatgcac
     7021 cgggaaatgc aggccacgcg gctgttggaa aagagccgcc tcatgagcca gcaggcctat
     7081 tttgccatgg agaaggggga ccttcaggaa gccgatcatc ttctggtcca ggccctgcaa
     7141 ctttgccccc aagacacgga ggcccgggcc cgctattccc aactcctttg gcgatgcggt
     7201 cgccgcgagg aggcccttga gcagatgaag atcgccctcc gcatggcccc acacgatgcc
     7261 gaattgcact tccgcattgc cgagcaatac ttggaactag gccagttgac cgaggctcag
     7321 gcgcatatcc aaaggggctt ggacatcgct cgggaaaacc ccaacggctg ggtccttcag
     7381 ggacgcctgg ctttgatgcg caatgatcct gacgcggcgt tgcgggcgtt ccatcaggcg
     7441 gcgggtatcc gcccccagga ccgtgacatc ctggaatgga tcgccaaagc ctatctggcc
     7501 cgaggtgata ccgatcaggc cctcgccgtc cgccagcgga tcatggatac cttcacaccg
     7561 ggggaggagt cagaacaggc cctagtagcg ttggccgagg cctaccaggc cgtccggcgc
     7621 tacgcttcgg cggctgaaat ctacctccag gccgcccaaa agagtgatca cccggaaccg
     7681 ctccttctcg ccgcagccga gaattactac cgggccggcc agcctgggca ggccctgggc
     7741 atcacagaac aaatcttggc ctccaatccc caaaaccatc gtgcccgcga tttacaaggt
     7801 cgcctccagg gtgtcaccgt agcctcccaa tcgcccatct ttcggtgaac gcacctggcc
     7861 gaaaatagcc gaccatatcg ttccggttgc acagagcacc accttgggcc gagatacccc
     7921 ttaccagccc gcgatgcccg ggataatcac ggggtataat tccaaaggtt acctgctttg
     7981 cctattctgc cccgtccctg aaggaatagt cgaactcgct catgtctcac ccgtcggata
     8041 gccgcgacga tcatgcacca ggggaaggcg ttccgccccc tggccaggat gaacgtgcgg
     8101 cagatgaggc ggcagcgata caatccaaac aaccggcgtt atccaaacct gcggaacaac
     8161 cctcccaacc cgccgcgaag gacgtgccgc caaagcggaa gcgaccggta gtgcttcggg
     8221 caccgttggc taagtctgcc cgcttggtgg aggaggccca aatcgcggcc atgctcccga
     8281 ctgaggtggc gacgccaatc acgccgcaca caccacgtag tcgggatcgc taccgctttt
     8341 tcgccagttg cttgatcagt gccgtggccc acgctgccgt gctcatcgcg ctttcgttat
     8401 ggatgttgaa cctgccgcag ggactggggc ccaccagtct ttttgccacc tgggcaggac
     8461 aagaatccct ggaaggcccc gaggcagagc ttgtcctcca gtcggaatcg caagagttga
     8521 ccccaaagga gaccgagctg gcccttcaac agtcgcggat caacgtcttg gagagctccc
     8581 ctctcgaaga gaacaattcc gatgaaccca ctgcgcaggt ggcggacgtc ggtgatcctg
     8641 cgcggccatg gggagttcaa ggggccacct tgatgcagtc tgtcgagcgc ccaacaggtg
     8701 gcggcttgga aggccgatct ccagagatgc gacaaaaact ggcccaaagt ggcggaggaa
     8761 cagcccagag tgaggaggcc gtggaacgcg gtcttcgctg gattgccgcc catcaggacc
     8821 aagatggcgg ctggagcttc gacctgaaaa aaggaagttg ccggggccag tgtcgaaatc
     8881 cagggacaga acctaatacg gcggcggcca cggccatggc cttgttaccc tttctcggag
     8941 cgggctatac ccatcgggaa ggaatctatg aggacaccct tcaccgcgga ctgtactacc
     9001 ttctgcgtcg tggacgtgtc acagagaatg gcctggactt tcaagagggc acgaagaatg
     9061 ggatgtatgc ccagggtttg gcggcgttgg tcctttgcga ggcgtatgcc atgaccaaag
     9121 atccggccct taaagaccca gcgcagcagg caatccggtt cattgaatcc gctcaagaca
     9181 aaaaaggcgg cggctggcgc tatcggccag gcgaacccgg tgatacgacg gtcaccggct
     9241 ggcaattgat ggccctgaaa agcgccgaga tggccggctt gaaggtcgat cgctctgtgc
     9301 attacggggc gcagcgattt ctcgattccg tggccagcga tgacggttct cagtacggtt
     9361 atctcgacaa aacgccgcgg aggagcacca cggcgattgg tcttctctgt cgtatgtacg
     9421 gtggctgggt gcgtggcaat ccgcccctgg tccgcggcat cggccatctc gcctcatggg
     9481 gaccatcggc caccgacatc tactacaact actacgcgac gcaggtactt cgtcattggg
     9541 gtggctcaga ctggaaacgc tggaacgacg ccatgcgcga gtatctcatc aacatgcagg
     9601 acaccaaggg acatgaatca ggaagctggt acttcgacga tgaacactcg cgggtcggcg
     9661 gccgacttta tacaaccgcc atggccgtca tgatcctcga agtgtactac cggtacatgc
     9721 ccctctacgg caagcaggtc ttcccggagt tatgaggccg tttgccccgc ggcccgcaag
     9781 gcgtcggcga ggatggcctc cgtgacccga tcgaatgacc agccggcctc ggcggccgcc
     9841 gcagcaaacc cggcatcagg actcaggcaa gggttggtgt tgatctccaa aatccaaggc
     9901 tggcctgccg catccacccg aaaatcgatt cgcgcataac cccgaagacc aaagacttgc
     9961 cagcactgcc gagctagggc ctcgagctgc aatagcagcg gttggtcctc cgcggggaag
    10021 acgaaacgcc ggggcgtcgc cacatactca aatgaatcgg catcccactt cgcgctgtag
    10081 ccgacgatct tcaatttctc gggtggaaag gcagagaagt cgatctcggc ggcaggaaac
    10141 acgataggcc cccgttcacc cgcaatgagc gagagattaa actctcgtcc ctcgatgtag
    10201 cgctccgcaa accacggttc gttcgactgt tgagagcgtc gggaaagctg ctctcgtagc
    10261 cgccacaaat cgtccctcgt gacaatcacc caaagttgct caatcagccc cgccgaggcg
    10321 tgcgaccagg cgcccttctg cagaaacgcc gggcgtgaat ctccttgggc gataagctcg
    10381 tcctggcttg tgacgtgatg ccagtgaccg gcgtggcgct tttgcaggtt atagaaaaca
    10441 cgggcgatgg gactgtcctc acgatgggcg tcgtcttccg tgagttgagc ccgcaaacat
    10501 ttctcctccc agcatgccag ccaatcgcga gcgacatcgt cgggcacaat ccactccggg
    10561 gtcggcagcc cggtggcacg cagaatcgac ttggtaatga gtttgtcccc agtcaaaaaa
    10621 agggcatctt ctgcacagcc cgtgaatggt acccccaatt cagttaatat gccggcaccc
    10681 caccattgga aaaagtcctt gccgaagaaa gattcgacaa gattgaaaac gacatccggt
    10741 tgcgtacgat ggaccagtcg gtacagatcg gcgggagagt gccccgccgg ctggatgatg
    10801 gcctcgtgcc ccagtcgtac caaggaatcg gctacggcgc cggcctggac caacacgtcc
    10861 ttatcgtctt gggaggcctc gggccccacc gtttgatgga cgaccagcac ccgcatgtca
    10921 cggccccgca ggacaacagt tcggcgtctg gcaagatgcc tccacccgtt ccaaggccga
    10981 ggcgataatc ctccgaatca actcccgaaa agataacccc ttagccttag caatcatagg
    11041 gaggtccgag tgctcgggat ggagaccggc aagggggttg acctccaaaa agtggggctg
    11101 gcccgtctcg tccgagcgga ggtccacgcg accggcgtcg cgcccgccaa tggcctgcca
    11161 tgccgccaag gccacacgtt ccgcctcggc cacctcggga tcactctccg ccgagggata
    11221 ccggtattcc acaagctctt cacaaaactc cttgttaacg taggaatagg catgggcctc
    11281 tgcctgcggg cggagaatga tctccattgt ccccaagacc ttggcctttt tgcccgttcc
    11341 gaggattccc acggtgaact ctcgccccga gaggtaccgc tcaaccagca ccggttgccg
    11401 aaaacgcgct agcagcgaga gacacgcggc ctcgagggcc tgcggcgtat caaccttgga
    11461 ctgcggcgaa atccctttcc ctgtcccctc cgccagcggc ttgacgaaaa ggggaaaggg
    11521 caaacggacc tgctcgacat cggcctcgca gcgaaccacc gcgtgggcag gggtgggcag
    11581 ccccgcctca tgaacgacca atttgcaaag ggccttatcc aggctgagca taagcacagc
    11641 cggatcggcg aacgtgtagg gaatctggta ggcatcgagg agggcaggca cctgggcctc
    11701 ccgtcccagg ccgtataacc cctccgcaat gttgaacacc aaatcccatc gatctccggc
    11761 ggccagtctc cgcacgaggt tatgaattcg cccgatccga tcgacctgat ggccctcggc
    11821 gcgcagggcg tcttcgatcg cttgaatcgt gtcatcgcga tcgaactcgg ccgtctcctc
    11881 ctcggagaaa ccttccgcca ggtattcact ccgtaagtcg taggtaaagc cgatcttcat
    11941 cagggtgcct ttgccaccgc agggggatag gaagatggtt ttccaagatc gttcgatttg
    12001 tggcccgtct gggatgcggt cgggcctacc ggcgtctggg cgagcaccga tccaccgggg
    12061 ccaccgggag ctggcgggtc cgcttccggc ccatctaccc gcactgggaa gatgttggcc
    12121 tcaaccgggg cgattttgtc ccgctcaccc gtatcaaaga gatgcggctc caggcggatt
    12181 tcggcgtaaa gctgccgcca ccagacctcc gccttttccc aggtcatacc cagcgcctga
    12241 agtaaatcct gcccgggagg cagcaagttc acggggcagc cgtccgccag atcgggcttc
    12301 aagccactgg ctgtgtattc caccggataa aggcgacaca caaggggccg aacttcccag
    12361 ggaagccggc aacccacggg tcctaggaag acacagtcgc ccgaggtgga acgccgcaac
    12421 acacgacgag aaccatcagg ccggaacacg caccttgccc attccgggtc gtcatcctgg
    12481 tccagatatt gtggatccac gggcttgcga aactcgaaga agtcaaagtg accaacgaac
    12541 gtcccgatcc gttgaacatc gcccggagtg acgtaaatct cacaaatttg gcagcatgtt
    12601 ttgccccgct gcgcgcaccg cgaacacaaa gatgcttcat ctcccatgat ccaccccttt
    12661 cttacttgtc taaggactgt tccaagggat cgggataccg gaaaatggca ccttcgtagt
    12721 tgcggagaat gagctcatca ccctcgcgac caacaacata attcggctgg agcggaatct
    12781 tgccgccccc acctggggca tcaatgacaa atgtgggcac ggcataaccc gaggtatgcc
    12841 cccgcaagcc ttcaatgatc tccagaccct tagcgacggg tgtccggaaa tgggcggaac
    12901 cgctgatcgg gtcgcactgg tacaaataat acggcttgac ccgcatcttg agcaactcat
    12961 gcaccaaccg tttcatggtc tcaacgttgt cgttgatccc tttgagcagg acggtctggg
    13021 agccgagcgg aatccccgcg tcggccaaac gcccacaggc cgtgtaggcc tcgggagagc
    13081 actcgtccgg atgggtgaag tggatactca tccacaaggg atgatatcgc cgcaacaccc
    13141 gacataactg gggcgtaatc cgctgcggaa ggactacggg caccttggtc ccgatccgca
    13201 ggaactccac gtgaggaatt cttcttagct cgccgaggag ccagtcaagt ttctcatcag
    13261 tcagcgtcag tgggtcgcct cctgagagaa ggacatccct caccgtgggc gtgtggcgaa
    13321 tgtaatccag ccccaaggcc agccgatctg cactcgattt cagctctcca tggccgacca
    13381 cacggcttcg cgtgcaataa cggcagtaag tcgagcagaa atcgtggacc aggaaaagga
    13441 cgcggtctgg gtaacggtgg acgatcccag gcaccgggct gtccttgtcc tcgcccaagg
    13501 ggtcatccgc ctcaccaggc gaacggacga actccgccgt gcggggaatg accgtcttac
    13561 gcaacggctg gtcgggatca tccggggaga ttaaggccat gtaatacggc gtgatcgcca
    13621 ccggcaattt ggccccggct tgctcgaggg ctgccctttc atccggcgaa agaacgagca
    13681 tccgctcaat ctcttccaaa gtgcggatgc ggtggctaat ctgccaatgc caatcgttcc
    13741 agagccggtc gggaacgtca cgatagaacg ttcgacggaa ttgtcgaacc cgaatgtcgg
    13801 tcctgacccg ccggaaagaa accggcggcg gaacaccgag cgaggacgaa tctgcacccg
    13861 aaaacgccgg gtaaggcagg agaccgttat cagaggtctc aaaaagggag agaacattca
    13921 gtgccgcacc ggaactcgga ggctcctctt cggcggcacg cacagacgct gtctcagcgt
    13981 caacggtggc cattgctacc cgatccctcg tcaatcacta cggtaaggcg acaacccatc
    14041 cacgttgccc gtgctccgac tcgcgggacg gccttcatgc cgtagtgacc aaaaccaatt
    14101 cataaaagaa ttatcggaag aagcaatctt gtcaagagga agaaatcgtt ccgtcgcccg
    14161 gaattttggt ttgcggctgt ttgtcggctg tcggagcacg ccaaacgggc tcgccaccta
    14221 gtcttttatt cggctccgtg aagctctgtc agcagtcgga gggctgcatt gaccagcatt
    14281 tcacccgacc cttccgcaca gcgagcactc accacttcgt actgaccctc cgcataggcc
    14341 gaccgattcg gaatgtaccc taccaaaccg ttggccaatc ccacgacaaa cgtttgacgg
    14401 aacggcgagg ctgccttgat gctcaaaccc aggtcggcaa aaacttcccc gggcag
//
LOCUS       DAOSUT010000103        14438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2520_length_14438_cov_8.832372, whole genome shotgun sequence.
ACCESSION   DAOSUT010000103 DAOSUT010000000
VERSION     DAOSUT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_14438_cov_8.832372"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="queC"
                     /locus_tag="PLD05_10270"
     CDS             complement(<1..598)
                     /gene="queC"
                     /locus_tag="PLD05_10270"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOP77868.1"
                     /translation="MPQNTDSVRAGPAQRKAIILLSGGMDSATTGAIAREEGYLLYAL
                     TVDYGQRHYRELEAAKRVAEFLGVAEHIILKVDLTAFGKSALTDTIDVPKDRPIAEMP
                     KEIPITYVPARNTVLLSLALAYAEARGAWDIFVGANAVDYSGYPDCRPEFISAFETMA
                     NLATKAGVEGTGRFRIHAPLLRLSKAEIIHRGLALGMNY"
     gene            726..1088
                     /gene="queF"
                     /locus_tag="PLD05_10275"
     CDS             726..1088
                     /gene="queF"
                     /locus_tag="PLD05_10275"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOP77869.1"
                     /translation="MSQGFRHILETFENQYPDRDYTIEIICPEFTSVCPKTRQPDFGT
                     ITILYSPDKLCVELKSLKFYLQAYRDVGIFYEHVTNRILDDLVAVLQPRWMKVIADFR
                     PRGGITTRVTAQYRAEHP"
     gene            1377..2840
                     /locus_tag="PLD05_10280"
     CDS             1377..2840
                     /locus_tag="PLD05_10280"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOP77870.1"
                     /translation="MSKDAYSGPLERIDETTWRIPRDYKPGMQVDGIIYADDRLIEQI
                     RSDQAPEQVANVAFLPGIQSASLAMPDIHWGYGFCIGGVAATDPQAGGVISPGGVGYD
                     INCGVRLIRTNLHVDDIKPRLGGLMNVLFQKIPAGVGKGGPYLFAGKQMDQILEEGCH
                     YLKKIGLATEQDIQHTEETGCIPGADASCVSQEAKKRGSDQCGTLGAGNHFLEVQVVE
                     EVFDPVAAQVMGLKEGGICVMIHSGSRGLGYQVCDDALKEMRRCPEKYHTELPDRQLA
                     CAPIESPEGQRYLAAMRAAANYAWANRQLLMWQTREAFSEFFGKPWEELQINLVYDVA
                     HNIAKMEKHIIDGKEKLVCVHRKGATRGFPAGHPDIPDRYRSIGQPVIIPGDMGRESY
                     VLVGLPAAMEKTFGTTCHGAGRVMSRNEAVRHARGRQIDKELAEKGIIARAHSRAGLA
                     EEHSDAYKDVSIVVDVVERAGLAKKVARLRPLGVVKG"
     gene            2881..3645
                     /locus_tag="PLD05_10285"
     CDS             2881..3645
                     /locus_tag="PLD05_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOP77871.1"
                     /translation="MGTLSRMIGFGSLIGLPALILLAMFCEGCGPPGTTNATPQPFNI
                     VGEAPSCCQEANQETSQQVSPAANPQGTQEVAQTPSVEGSLKPKEGGRIMKMKLTSPA
                     FEPGQPIPKQYTGEGADVSPPLEWSNVPEGTQEFALICDDPDAPTAEPWVHWVIYKIP
                     ADVTKLPEGIAKTPKLSVPPGALQGKNSWPSGQTIGYRGPMPPPGHGRHRYYFRLYAL
                     DKKLDLEPGLTKKQLLEAMKGHILGEGELMGTYERR"
     gene            3756..4490
                     /locus_tag="PLD05_10290"
     CDS             3756..4490
                     /locus_tag="PLD05_10290"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOP77872.1"
                     /translation="MPDRRRHRGPHPEDRQLFDAAMYPRLQAAFSDLCWLLDRGYAVT
                     SSLKLVGDRYELSARQRLALERCACTAAAVAARQSRLCPPQELAGRAICLDGYNVLTT
                     IEAALAGGVLLGGRDGTLRDMASMHGSYRKVAETQQAIELVGNTLASLGVSEAVWYLD
                     KPVSNSGRLAQLLRTLAAEHQWNWEVNIVPSPDHELQARGIPVATSDHVILDHCQSWI
                     NLARWIVERNVPNAWIVDFANAAPAS"
     gene            4612..5661
                     /gene="hemB"
                     /locus_tag="PLD05_10295"
     CDS             4612..5661
                     /gene="hemB"
                     /locus_tag="PLD05_10295"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOP77873.1"
                     /translation="MGHSRREELWVGQCRMPGFPLTRLRRLRYHPAIRRMIQATHLDP
                     RRWILPLFIHAKGDEKKPIKSMPGHFQLPLSGLRAEIKEIMQLGLGGVLLFGIPAHKD
                     AMGCDALRDSGIICQAIKAIKDVAPDLLVITDECFCEYTDHGHCGVLGRAGNQDTVDN
                     DKTLRMLAWQAVAHARAGADMVAPSGMMDGMVQAIRSALDEAGFTHLPIMSYSAKYAS
                     AFYGPFREAAESAPRFGDRRSYQMDPAAAAEQALREVELDVAEGADIIMVKPALAYLD
                     VIRMVKDNFPGLPLAAYNVSGEYSMVQAAAEKGWLDPRRIALEMLTAIHRAGADIVIT
                     YWAKDAAHWLDSSDL"
     gene            complement(5687..6442)
                     /locus_tag="PLD05_10300"
     CDS             complement(5687..6442)
                     /locus_tag="PLD05_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOP77874.1"
                     /translation="MTEIREPRIVAAAEREMQKWRTAQQIADRIIRFDTAHGAQQKKI
                     GPYITISRQAGAGAEQIAHAVGERLGWEVVDKNVLDAIAKHFCLERSQLENLDESSGN
                     WLRDTLFLWLDPHIISADKFLACLEKVVWMAARKGNVVFVGRGVRFLLPDEHGLAVRL
                     VASEKYRVERIRREKGLSEAAARRFVHELEQTRRQFVQRFFHKDVDDPQWYDLVINVE
                     RWGVEETANLIVTAWRAREEQMKTVAKPAEAVV"
     assembly_gap    6650..6749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6750..7569)
                     /locus_tag="PLD05_10305"
     CDS             complement(<6750..7569)
                     /locus_tag="PLD05_10305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP77875.1"
                     /translation="MAGISRRGFLAGLGAVGGVALSGKWAIADPHKPTLKSGVDMITL
                     GRTGLKTSVLGVGTGTVGGSQQLAMGSEGFGKMLRYALERGVHYIDTADNYNTHIFIR
                     LALQATPRVPREKYFIQTKTRARTAEVLHADIERFRRELRVDYIDSVLMHCMTTGDWP
                     TVMRPVMDALYDEKQKGRVKAVGVSCHGWEPLVASVDVDWCDVQLVRINPFGAKMDGE
                     PKDVAAVIKKMHDAGRGILGMKIYGETGFNSREERLQSLKYVLGLGCVDCFTIGF"
     gene            complement(7845..8219)
                     /gene="rplT"
                     /locus_tag="PLD05_10310"
     CDS             complement(7845..8219)
                     /gene="rplT"
                     /locus_tag="PLD05_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOP77876.1"
                     /translation="MRTKTSATRRRRVKRLFRRAKGFVGGRRRLLRTVKETLVRAGVY
                     AYRDRRARKRVFRRLWITRISAAVRQRGMLYSRFIDGLNKAGIKLDRKALAEMAISDP
                     EGFDQVVEKAKEALQAAQSPLW"
     gene            complement(8387..8581)
                     /gene="rpmI"
                     /locus_tag="PLD05_10315"
     CDS             complement(8387..8581)
                     /gene="rpmI"
                     /locus_tag="PLD05_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOP77877.1"
                     /translation="MPKQKTKKSAAKRFKITGTGKVKHRSAGTSHLATRKSKKRRRQL
                     RGTDVLSSVEAKRVIPLIRT"
     gene            complement(8808..9356)
                     /gene="infC"
                     /locus_tag="PLD05_10320"
     CDS             complement(8808..9356)
                     /gene="infC"
                     /locus_tag="PLD05_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOP77878.1"
                     /translation="MFVGRFQKERSLAIEEKNYRVNERIRSPSVRLIGPEGQQLGVVS
                     REQALAAAREAGLDLVEVAPNENPPVCRILDFGKFKYLQKKKQQKSQAAQIKVKEIRV
                     RPGTGEADLNVKINRAREFLTKKDKVLVSVMFRGREAAHMEEGEKVLNLIIERLADVG
                     KVEGAPSRQPKRIVCTIVPAVK"
     gene            complement(9546..11519)
                     /gene="thrS"
                     /locus_tag="PLD05_10325"
     CDS             complement(9546..11519)
                     /gene="thrS"
                     /locus_tag="PLD05_10325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOP77879.1"
                     /translation="MLRVIFPDGSAKEYRERVRPIDIAAELGPRLAKSAVAAMVNGSV
                     VDMTAPLPQEGEVQLRILTERDHEALNVLRHSAAHVMARAVLRLFPKAKLAFGPPVEN
                     GFYYDIELERSLTDDDLPRIEEEMAKIIAQDEPFERIEVPWERAVEICRDLDQPFKVE
                     HLQEGLASERVVSFYRQGEFLDLCEGPHVPSAGWIKAFKVLSVAGAYWRGDASRQQLQ
                     RLYGTAWFSQKDLDEYLRRLEEAKKRDHRVLGKRLELFTIEPMVGSGLILWLPKGAII
                     RRQLEDFLFGELIRWGYQAVYTPHIGRVELYQTSGHYPYYADSQFPPIQMADGERYLL
                     KPMNCPHHIMIYKSRPRSYRELPLRLAEFGTVYRFEQSGELTGMTRVRGFTQDDAHIF
                     CTEEQVADEFRGCIQMTQFVLKTLGFTDYRVRLSLRDPKSDKYVGDPEVWERAESALK
                     QVCQEMDLPNLETAIGEAAFYGPKADFIVSDCIGRQWQLGTVQLDYNLPSPQRFNLEY
                     IGPDNRPHQPVMIHRAPFGSLERFTGILIEHFAGAFPLWLAPEQIRVLTVSEKFDEYA
                     RRVEGRLREAGLRVSGDYRAEKVGAKIRDAQLEMIPYMAIVGAREQEQGTVALRDRLK
                     GDLGAMPLDEVISRLQEEIRLKLIRRSDERKNG"
     gene            12092..13060
                     /locus_tag="PLD05_10330"
     CDS             12092..13060
                     /locus_tag="PLD05_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77880.1"
                     /translation="MAPSQSLFYYDEGLKLLPINLAIDVRRPHAQAFVSHAHADHIAR
                     HGYTLCTAETAAFLGLRLGPRPTREMSYGKNIDWGGVSLTVYPAGHCLGSAMLLVEHQ
                     GQRLLYTGDFKLRPALTTPAAQPVKADILIMETTYGEPRYRLPPEDEVIEDLACCIRD
                     TWDRGRCPVIMAYALGKAQEVSRRLSEAGIGVVQHSSVATISRIYEQFGVSVGNYRPY
                     DGRLERGEVLITSPQQRWDCPTESVVRIAVTGWALDPSAKYRLGADVVFPLSDHADYE
                     ELFQMVQTVQPQLVYCTHGPATFVRDLMERGWGARLLGRPTQKLLF"
     gene            13383..13574
                     /locus_tag="PLD05_10335"
     CDS             13383..13574
                     /locus_tag="PLD05_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77881.1"
                     /translation="MSICERKRELRRKRHRREKYRIFKRKLLTANASERAIIAEKIRK
                     LAPDPEVVLKNLGLLESQR"
     gene            complement(13665..>14438)
                     /locus_tag="PLD05_10340"
     CDS             complement(13665..>14438)
                     /locus_tag="PLD05_10340"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOP77882.1"
                     /translation="LFCDLRGFAHETERQADQLLPLLDRISAALEVMTRRILEHGGVI
                     GDFQGDAAMGFWGWPLPREDRVLAACRAALAIRHDFSQAAQTPGHPLAGFHIGIGLAT
                     GRVVAGKIGTPDHAKITVFGPPVNLAARLEGITKLIHVPILMDDETAKAARTLVTSEI
                     GRIRPIARIRPYGLDQAVLVHELLPPECEYPLLKEHHLHLCEQAVEAFIRGDWERAFS
                     LFHQLPAEDRGPDFVTAYIAQHHRVPPNSWDGTIVMSSK"
BASE COUNT         3187 a         4200 c         3843 g         3108 t
ORIGIN      
        1 cgtaattcat tcccaaggcc aacccccgat ggataatctc cgccttactt agccgcaaca
       61 gcggggcatg gatgcgaaat cgcccggtcc cttccacccc tgcctttgtc gccaagttgg
      121 ccatcgtttc aaaggcggag ataaactctg gccggcaatc gggataaccg ctgtagtcca
      181 cggcattggc cccgacaaag atatcccacg ccccccgggc ctccgcgtac gccagggcta
      241 gcgacaacaa aacggtattg cgggctggga catacgttat cgggatctcc ttcggcattt
      301 cggcgatcgg acgatcctta gggacatcaa tcgtgtccgt caacgcactc tttccaaaag
      361 ccgtgagatc caccttgaga ataatatgct cggccacgcc cagaaactcg gccactcgct
      421 tggctgcctc caactcccgg tagtgccgct gcccgtaatc caccgtcagc gcatacagca
      481 ggtagccctc ctctcgggcg atggcccccg ttgttgcgga gtccattcca cccgatagga
      541 gaataatagc tttacgctgt gctggtccag ctctgacgct gtcggtgttc tgaggcattc
      601 acgcgattcc tgtggctgat tccctttttg tgcgggcaat cctgacatgc ctcgccacgt
      661 gtgggcagga gtcccccgct cctatatgat ggacagaaat gcccgatcgt ttaaggagga
      721 ctgctgtgag tcaaggtttt cgtcacattc tcgagacgtt cgaaaatcag tatcctgacc
      781 gagattacac cattgagatt atatgccctg agtttacctc ggtctgtcct aagacccgac
      841 agcctgattt cgggacgatt acgatcctat attcaccgga caagctctgc gtggagctga
      901 agagcctaaa gttctatctt caggcgtacc gcgatgtagg tattttttac gagcatgtca
      961 cgaatcgcat cctcgacgat ctggtggcag ttctgcaacc acgctggatg aaagttatcg
     1021 ccgacttccg tccccggggt ggtatcacga cccgcgtcac ggcccagtac cgtgctgaac
     1081 acccataaac tcgtaaacct gtgcctatct gaggccagat atcttagcca gacatcttaa
     1141 atgatttttg acagtcgtcc cagtctcata gaatgacaag tggacgcggg atgaatgttg
     1201 gcccggttca gaatgttaaa ttggcgaggg tctcagcttc cccgtcagga cgctaccatg
     1261 ccgaccaaca taaggccctc atcatggaaa gggaaccgca actcttttga cacctcgcat
     1321 ttgggcagat gtcagaagaa tcagagaacg tttaggcctt aaaaggaggc tgaatcatga
     1381 gtaaggacgc atactctgga ccgctcgaac gcatcgacga gacgacgtgg cgcatcccac
     1441 gggactataa accgggaatg caagtggacg gtatcatcta tgcggatgat cgcctgatcg
     1501 agcagattcg ctccgatcag gcccccgagc aggttgccaa tgtggcgttc ttgccgggga
     1561 ttcagtccgc aagcctagca atgcctgaca tccattgggg ctatggattc tgcatcgggg
     1621 gtgtcgcagc gaccgatccg caggccggtg gtgtgatttc accagggggt gttggatatg
     1681 acatcaattg tggtgttcga ctgattcgga ccaatctcca cgtggacgac atcaaaccac
     1741 gattgggcgg tttgatgaat gtcctttttc agaagattcc tgccggtgtg ggcaaaggtg
     1801 gaccctatct ttttgccgga aagcaaatgg accaaattct cgaggagggt tgccactatc
     1861 tgaagaagat cggcctggcc accgagcagg acattcagca cacagaagaa acagggtgta
     1921 tcccaggggc cgatgcctcg tgcgtcagtc aggaagcgaa gaagcgtggc agcgatcaat
     1981 gcgggacact gggggcggga aatcactttc tcgaggtgca ggtggtggaa gaggtctttg
     2041 accccgtagc cgcccaggtt atggggctaa aagaaggcgg catttgcgtg atgatccact
     2101 ccggttcccg cggtctgggc tatcaagttt gcgatgacgc cctcaaggaa atgcgtcgct
     2161 gccccgagaa atatcacacc gaacttccag atcgccaatt agcctgtgcg cccatcgaaa
     2221 gcccggaggg acaacgatat ttggcggcca tgcgggcggc ggccaattat gcctgggcca
     2281 atcggcagct cttgatgtgg caaacgcggg aggcctttag cgagttcttc ggcaagcctt
     2341 gggaggaact gcaaattaac ctcgtctatg acgtggcgca taatatcgcc aagatggaaa
     2401 agcacattat tgacggcaaa gaaaaactgg tgtgcgtcca tcgcaaaggg gctacgcggg
     2461 gattccccgc cgggcatccc gacatcccag atcgttatcg atcgatcggt cagccggtga
     2521 tcatcccagg ggatatgggg cgagaaagtt atgtcctggt gggattgccg gcagccatgg
     2581 aaaaaacctt tggcaccacc tgtcacgggg caggtcgggt gatgagtcgc aacgaagctg
     2641 tgcggcacgc tcgaggacga caaatcgaca aggaacttgc cgaaaaaggg atcattgcgc
     2701 gagcgcatag tcgggcgggg ctcgccgagg aacactccga cgcttataaa gatgtcagta
     2761 tcgtagtcga cgttgttgag cgggcggggc tggccaagaa agtcgcccgc ctgcggcctt
     2821 tgggtgtcgt caaaggataa ccctttttca aacctgtgtg atcttaaggg agggtcagcc
     2881 atggggactc ttagtcgtat gattggcttc ggcagcttga tcggcctacc ggctctcatt
     2941 ctcctggcga tgttttgtga aggatgtgga ccgcccggta cgacgaacgc cacgccgcag
     3001 ccgtttaaca tcgttgggga agccccctct tgttgtcagg aagctaacca agaaacaagc
     3061 caacaggtat cacctgccgc caatccccag ggcacgcaag aggtagctca gacaccgtct
     3121 gtggaagggt ccctcaagcc aaaagaagga ggtcgtatca tgaagatgaa actgacaagt
     3181 ccggcatttg aacccgggca gccaattccc aagcaataca caggggaagg agccgacgtg
     3241 tcgccaccct tagagtggtc aaatgttccc gaggggacgc aggaattcgc gcttatttgc
     3301 gatgatccag acgccccgac ggcagaaccg tgggtccatt gggtgattta caaaatcccg
     3361 gcggatgtga ccaagcttcc cgaaggaatt gccaaaacgc ccaaactttc agttccgcca
     3421 ggcgccctcc aggggaagaa ctcttggcca agcggccaaa cgatcggcta tcgcgggccc
     3481 atgccgccac caggtcatgg tcgccatcgg tactacttcc gtctgtatgc tctggacaag
     3541 aagctcgatc tggaaccagg ccttaccaaa aaacaactgc tggaggcgat gaaaggccat
     3601 atcctgggag aaggcgagct gatgggcact tatgaacgcc gctagccccg gctacttccg
     3661 atagccgaga ctggctcggc ggcatgccgc ctccaacgtg ttcgtcatgg tccgtaaggg
     3721 ctgggttttg aacttgattg agcagcaggt gaactgtgcc ggatcgccga cgacatcgtg
     3781 gtccccatcc ggaagatcgg cagctattcg acgccgccat gtatccccga ttgcaggcag
     3841 cattttccga tctttgctgg ttgctggatc ggggttacgc ggtcacctcg tcgttgaaac
     3901 tggtgggcga caggtacgaa ctttcggccc ggcaacgatt ggctcttgag cggtgcgcat
     3961 gcacggcagc ggcggttgcc gcaaggcagt cgcggttgtg tccgccgcag gagcttgctg
     4021 gtcgtgccat ttgtcttgat ggctacaacg tgctgacgac aattgaggcg gccttggcag
     4081 gaggggtact cctcggagga cgtgatggca ccctccgcga catggccagc atgcacggct
     4141 cctatcgaaa ggtggccgag acccaacagg ccatcgaact ggtgggaaac accctggcct
     4201 ccctcggtgt gtcggaagcc gtgtggtatc tggacaagcc tgtgtcgaat agcggtcggc
     4261 tggcacaact cttgcgcact ttggcggccg agcaccagtg gaattgggag gtcaacatcg
     4321 tacccagtcc cgatcacgag ttacaagcga gaggtatccc cgtggccacg tccgatcatg
     4381 tgattctcga ccattgtcaa agctggatca atcttgctcg ctggattgtg gaacgaaacg
     4441 ttcccaacgc atggatcgtg gattttgcca atgccgcgcc agcttcatag agttagcgac
     4501 catagtggcg cacgttggtg ttacgattta gggaatgcgt gaagaattgc tacaacgttg
     4561 ggcggggtat tcgctcggag atttgattaa cgtctgaatg aggagatcaa tatgggtcat
     4621 tcacggcgtg aagagctttg ggtaggacag tgccggatgc caggcttccc cctcacacgc
     4681 ttacgtaggt tgcgttatca tccggccatt agacggatga tccaggcaac ccacctggac
     4741 ccacggcgct ggatcctgcc tttatttatt cacgctaagg gcgacgaaaa gaaacccatc
     4801 aaatcgatgc ccggacattt tcagttaccg ttgtcgggcc tccgcgcgga gatcaaagag
     4861 attatgcaat tgggattagg tggggtcctc cttttcggca tccctgcgca caaagacgca
     4921 atggggtgcg acgccctgcg tgactctggg atcatctgcc aggcgatcaa ggccatcaaa
     4981 gatgtggccc cagatctgct tgtcatcacc gacgagtgct tttgcgaata caccgaccat
     5041 ggacactgtg gcgtcttggg tcgggccgga aaccaggaca cggtggacaa cgataagacg
     5101 ttacggatgt tggcgtggca agcggtggcc catgcccgcg caggtgccga catggtggcc
     5161 cccagtggga tgatggacgg aatggttcag gctattcgta gtgccctcga cgaggcaggc
     5221 tttacgcatc ttcccattat gagctatagc gccaagtatg cgagcgcgtt ttatggtccc
     5281 ttccgggagg ccgccgagag tgccccaaga tttggcgatc gccgaagtta ccagatggac
     5341 ccagccgcgg ccgccgagca agccctccgt gaggtcgagc tcgatgttgc cgagggggct
     5401 gacatcatca tggtcaagcc tgccttggcc tatctcgatg tcattcggat ggtcaaggac
     5461 aattttcccg gtctgcccct ggctgcctat aacgtctccg gtgagtacag tatggtccaa
     5521 gccgccgcgg agaaaggctg gcttgacccg agaagaatcg ccttagagat gctcaccgcc
     5581 atccaccgag ccggagcgga tatcgtcatc acctactggg ccaaagacgc ggcccactgg
     5641 ctagactcat ccgacctata ggtcggcagc ctcgagagac tcacgcttac accaccgcct
     5701 ccgcaggctt ggcgactgtc ttcatctgtt cctcccgcgc ccgccaagcc gtgacaatca
     5761 aattggccgt ttcttccacg ccccaccgct ccacattgat gacaaggtcg taccactgag
     5821 ggtcatccac gtccttgtgg aagaatcgct ggacgaactg acgccgcgtc tgctctaatt
     5881 cgtggacaaa gcgacgggcg gcagcttcgc tcaatccttt ttcccgacgg atacgttcca
     5941 ctcggtactt ctccgaagcc accagtcgca ccgccagtcc atgttcatcg gggaggagaa
     6001 aacgaacacc tcggccaacg aacacgacat tccccttgcg cgcagccatc cagacgacct
     6061 tctccaagca ggccaggaac ttgtcagccg aaatgatgtg tggatctagc cacagaaata
     6121 atgtgtcgcg aagccaattc ccggaagact cgtccaagtt ctcaagctga ctgcgttcca
     6181 aacaaaagtg cttggcaatg gcatcaagga cgttcttgtc cacgacctcc caacccaacc
     6241 tttcgccaac ggcatgggcg atttgctcgg ccccggctcc agcctggcga gaaatggtga
     6301 tatacggacc aatcttcttc tgctgagcac cgtgagctgt atcaaagcga atgatgcgat
     6361 ccgcaatctg ctgggccgtc ctccatttct gcatttcccg ctccgccgcc gccacaatcc
     6421 tcggttcgcg gatctctgtc atagggccct cccatcatta acgagaagct cagaaaagga
     6481 aaagtcacat cggccttgca ccttggccct ctgtggtcag atcactgccg atccctcaat
     6541 atctgtccac ctagattata cccaaattgg aggtaacgac caataaaaaa acgtgaccct
     6601 ttctttgcgc agactcgcgt cccggcgtct tcacgagacg accaaccatn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gaacccgatg gtaaagcagt ccacacagcc
     6781 caagccgagg acgtatttca gcgattgcag gcgttcctca cgagagttga aaccggtttc
     6841 gccataaatc ttcatgccaa ggatgccgcg acccgcatcg tgcattttct tgatgacggc
     6901 tgcgacatct ttcggctcgc cgtccatctt cgctccgaag gggttaatcc ggaccaattg
     6961 aacatcgcac caatcgacat cgaccgaggc cactaggggt tcccagccat ggcacgaaac
     7021 accgacggct ttgacccgcc ccttttgttt ttcgtcgtaa agggcgtcca tgactggtcg
     7081 cattaccgtg ggccaatctc ctgtggtcat acagtgcatg agcacagaat caatgtaatc
     7141 cacccgcagt tcacggcgaa atcgctcgat gtcggcgtgg aggacttcgg ccgttcgagc
     7201 ccgcgtcttc gtttggatga agtatttctc gcggggcacc cgcggggttg cctgcaaggc
     7261 caaacggatg aaaatatgcg tgttgtaatt gtctgcggtg tcaatgtaat ggactccgcg
     7321 ttccagggca taccgcaaca tcttgccgaa gccttcactg cccatcgcca attgctggct
     7381 gcccccgacc gtccctgtgc ccactccaag gacactcgtc ttcagccccg tccggcccag
     7441 cgtaatcata tcgacgccac ttttcagggt aggcttatgc ggatcagcaa tagcccattt
     7501 ccccgaaaga gctactccac ccacagcccc taaacccgcc aaaaacccgc gacgcgaaat
     7561 gcctgccata ggaagtcctc ctattcgttt ccctattcac acccaatcaa ctacgtgtaa
     7621 cccatgaacg ttgacgcggc cttttctagg tctttcggaa cacggcacac taaaacactg
     7681 ccttattttg gccaccgcga tgccccattt tatacgagaa gtttgccaaa ttccaccaac
     7741 tgtggctgac attttacaag gacagtcgct ccgtctggca ataaaaaacg cccgagatat
     7801 tcgggcgttc catatggtca ccgttttgcc gagtgcgaat caaattacca caggggggat
     7861 tgggcagcct ggagggcctc tttggccttt tcaaccactt ggtcaaaacc ctctggatcc
     7921 gaaatggcca tttcggccag ggcctttcgg tccagcttaa taccagcctt attcaaacca
     7981 tcgataaatc ggctatagag catcccccgc tggcggaccg ccgcgctgat gcgtgtgatc
     8041 caaaggcggc ggaatacccg cttgcgtgca cgccggtctc gataggcgta aaccccggcc
     8101 cgaaccagcg tttctttcac cgttcgcagg agccgccgac gtccgccgac aaacccttta
     8161 gctcgacgga ataagcgttt aactcgacga cgacgtgtgg cactcgtttt agtacgcatg
     8221 atcaatcatc tcctcaccac ccgggaacaa ggcctcctcg gccgcagccg aggacgtttc
     8281 ctgcccgtcc cgtgaatctc attctttgtg agccttaacg tttcccttga tcaatcgccc
     8341 actgcatttg aaaagctggt ggcaagaccg aaactttgtg cgatcctcag gtcctgatca
     8401 aagggatcac ccgcttggct tccaccgaag aaagcacatc ggtgccgcgc aactggcgac
     8461 gtcgcttctt tgattttcgc gtagccaaat ggcttgtgcc ggcactgcga tgcttaactt
     8521 tgcccgtgcc tgtgatcttg aacctcttgg cggcggattt tttcgttttc tgctttggca
     8581 ttttgacagt ccaactacga tcgtgatgac ctgctacgct ttcccgtgac aaaaaaaccc
     8641 cggctcccct ctccctcaaa gctgccgagc gtcgaaaaca tgaattctac ccatttcacc
     8701 ggaaccgcgg aaggccctcg gcgaaaaggc tttctccggc gaggctcaaa cacctcgccc
     8761 cggcggcata ctgcccggcg taacccggtc tccagcagta cgcacggtca cttaacggcg
     8821 gggacaatcg tgcagacgat gcgtttcggt tgacggctgg gagccccctc gacctttccc
     8881 acgtcggcca gacgctcaat gatcagattg aggactttct caccctcttc catgtgggcg
     8941 gcttcccgtc cccgaaacat cacggacacc aagactttgt ccttcttggt caggaactcc
     9001 cgggcacggt tgatcttcac attcagatcg gcttcccctg tgccaggtcg gacacggatc
     9061 tccttgacct tgatctgcgc cgcttgggat ttctgctgct ttttcttttg caggtacttg
     9121 aacttgccga aatccaaaat ccggcaaacg gggggattct cgttgggggc aacttctaca
     9181 agatcaaggc cggcttctcg ggccgctgcc aatgcttgct cgcggcttac cactcccaac
     9241 tgctgacctt ctggaccaat caaccgcacc gatggactgc ggatccgctc attgacgcga
     9301 taattcttct cctcgattgc tagactcctc tctttctgaa aacgaccaac aaacaaacct
     9361 actcgccatt ccaatgaaca ctcggccagc ctcttccagg ggcccttaat cttttcgtta
     9421 gagtatgacc agagaaaaag gcaaagtcaa cacctttggg taatatttgc catgattatc
     9481 tccggccttg tcgtggatgt cccctgttca caaccaagtt gactcgtcgt gtccagaaaa
     9541 cggtctcatc cattcttcct ctcgtcagat cgacgaataa gcttcaaccg aatctcttcc
     9601 tgaagtcggc tgataacctc atccaaaggc atggccccca ggtccccctt cagccgatcg
     9661 cggagggcca ccgttccctg ctcctgctcc cgcgccccta cgatggccat gtagggaatc
     9721 atttcgagct gggcatcgcg aattttcgcc ccgacctttt cggcacggta gtcccccgaa
     9781 actcgtaacc ctgcttcgcg gagtcggcct tccacacgcc gcgcgtactc atcgaacttt
     9841 tcgctcacag tcagcacgcg gatttgctcg ggggccaacc aaagcggaaa cgccccggcg
     9901 aaatgctcga tcaagatccc tgtgaaacgt tccagcgaac caaagggggc acggtggatc
     9961 atcaccggct gatgaggtcg gttatcggga ccgatatatt ccaggttaaa ccgctgcgga
    10021 ctgggcaggt tatagtcgag ctggacagtc cccagttgcc actgccgtcc gatgcaatcg
    10081 gagacaatga aatcggcctt gggcccataa aaggccgctt ctccgatggc tgtttccaaa
    10141 tttggcaggt ccatttcctg acagacctgt ttcagcgcgg actcagcccg ttcccaaacc
    10201 tccgggtcgc cgacgtactt gtcactcttg ggatcacgca ggctcagcct gacgcggtaa
    10261 tccgtgaagc ccaacgtctt caaaacgaac tgagtcatct ggatgcaacc gcggaactcg
    10321 tcagcgactt gctcctccgt gcaaaagata tgggcatcat cctgggtaaa gccgcgaacg
    10381 cgggtcatgc ccgtaagttc tcccgattgt tcaaaccgat agaccgttcc aaattcggca
    10441 agccggagcg gcagttcccg ataactcctc ggtcgagact tgtaaatcat gatgtgatgc
    10501 ggacaattca tcggcttcag aagataccgc tccccatcgg ccatttggat gggtggaaac
    10561 tgactgtcgg catagtaggg gtagtgaccc gaggtttgat aaagctccac ccggccaatg
    10621 tgcggcgtgt aaactgcctg ataaccccag cggatcaatt ccccaaacag aaaatcctcc
    10681 aattggcgac ggataatcgc cccttttggc agccaaagga tcaagccgga gcccaccatc
    10741 ggttcgatgg tgaagagctc caaccgcttg cccaaaacac ggtgatcgcg tttttttgcc
    10801 tcttccaaac ggcggaggta ctcatcgagg tccttttgac tgaaccaagc tgtcccgtac
    10861 agccgctgca actgctgacg cgaggcatca ccccgccagt acgcccctgc gaccgaaagc
    10921 actttgaagg ccttaatcca gccggccgag ggcacatggg ggccttcgca aaggtccaga
    10981 aactccccct gacggtaaaa cgagaccacc ctctccgagg ctagcccctc ctgaagatgt
    11041 tcaactttga agggttggtc caaatcccgg caaatttcga ccgccctttc ccaaggcacc
    11101 tcgatccgtt caaagggctc atcctgggcg atgattttgg ccatctcctc ttcgattcgg
    11161 gggagatcat catccgtgag acttcgttct agctcgatgt cgtagtagaa accattttcc
    11221 accggtggac caaatgccaa tttggccttg gggaaaaggc ggaggaccgc gcgtgccatg
    11281 acgtgggctg ccgagtgtct gaggacgttc agcgcctcat ggtcccgctc cgtgagaatc
    11341 cgtaactgca cctccccctc ttgcggcaat ggggccgtca tatcaaccac cgaaccattc
    11401 accatcgctg cgacggcact ttttgccaac cgtggaccca gttcggcggc aatgtcgatg
    11461 ggccgaacac gttcccgata ttccttggcg cttccgtcag gaaagatcac ccggagcatt
    11521 ttggtatatt ctccaaacaa cctattttga cactgggaaa gattgaggaa gccacgccgg
    11581 tgcgtggtat aaacctatct agtatcgtct tggcgagcga atattaaaag ggcaacctgg
    11641 gcaggttatg cgcacctgaa gcccaatcat aaggtcggag cgtcttttca ctagcggtgg
    11701 gtttgtcaca cttcgtaggg gcaggcccct gtgcctgccc gatctgatgg aaagcgaggc
    11761 ccactcgtgg gcgtttaaac catccataca gcgatatcca cgccggagcc ctctcgcccg
    11821 gccggttggc gattggggct ggtattatcg tggccgcgac ggcgtgcctc tcgaaatcca
    11881 acaaacgagt aaccccacgg gttggaccta ctgacacacc gcctcctggc cgaaggtcac
    11941 gctggcttgg cctggtcaaa ttcattgcgg caaacggcca agtcaggacc ttccttctgg
    12001 tggaaggagt tgggacaatc atcggtaagc gtcccatggc tagttcttgg gcccggggag
    12061 acaacaaaca attttcacga gaaacgtcat cgtggcaccg tcccaatccc ttttctatta
    12121 cgatgaaggc ctaaagctat tacccatcaa cttggccatt gatgtccgac ggccccatgc
    12181 ccaggccttt gtttctcatg ctcacgccga ccacattgcg cgacacggat acacattatg
    12241 cacggcagag acggcggcct tcctcggcct gcgccttggt ccacggccga cgcgggaaat
    12301 gtcctacgga aaaaacatcg actggggtgg cgtctctctg actgtttatc ccgcgggcca
    12361 ttgtttgggg tcggccatgt tgcttgttga gcaccaagga caacggcttc tttacactgg
    12421 cgattttaaa ctacgccccg ctttgaccac accggctgcc caacccgtca aggcagacat
    12481 cctcatcatg gaaacaacct acggggaacc gcggtaccgc ctgcctccgg aagatgaggt
    12541 gatcgaggat ttggcatgct gcatccgcga cacgtgggac cgaggtcggt gccctgtcat
    12601 catggcttat gccctgggta aggcccaaga ggtgtctagg cgtttgagtg aggccgggat
    12661 aggtgttgtc caacactcgt ccgtggcaac gatcagccgg atttatgagc aattcggagt
    12721 ctcggtagga aattaccgtc cctacgacgg acgcttagag cgcggtgagg tgctgatcac
    12781 atcaccccaa caacgttggg actgcccaac ggagtcagtc gttcggattg ccgtcacagg
    12841 ttgggccttg gacccttcgg ccaagtaccg tcttggggcc gatgttgtgt ttcccctctc
    12901 tgatcatgcc gactacgaag aactcttcca gatggtgcaa accgttcagc cccaacttgt
    12961 ttattgcacg cacgggcccg cgacattcgt gcgggatttg atggaacgtg gctggggcgc
    13021 ccgccttttg ggtcgcccca cacaaaagct cctgttttga tagcccgcct cgctgtgctt
    13081 caccgtcttc agtttggcgg ctcatctgcc gcctcgttac cgaaacgaaa gcaaccataa
    13141 cgcgaggagc cagcggaaga ccacaggcca acgcccacaa aaaaaggcga tgacggtcgc
    13201 cccacatttt ccctgtcccc cctgccgtta aaatcgaacg ccaaccgggt ctattgacgt
    13261 cctgcgaagc cacgagtaaa aggaacgtga tggggtggtg gtagtctggg aaaaagctcc
    13321 catgaggagc gcgccagatt ggcacactat gaatcattag tttttctcaa aggaggttga
    13381 ttgtgtcgat ttgtgaacgc aaacgagaac ttcgccgaaa gcgccatcgc cgggaaaagt
    13441 atcgcatttt caagagaaaa cttttgacag caaatgcctc ggagcgggca attattgcgg
    13501 aaaaaattcg aaaattggcc cctgacccgg aagtggtgtt gaagaatctc ggcctcttgg
    13561 agagccagcg ttgattcgcc ccaggagcaa accttcttgt gacgctcctt cttttctgac
    13621 tcgccgcgaa gacggcccat aagacgaagg cgagatcaaa atggctattt actcgacatg
    13681 acgatcgttc cgtcccagga attgggcgga acacggtgat gttgtgcgat gtatgcggtc
    13741 acaaagtccg gaccgcggtc ctcggcaggc aattgatgaa agagggagaa ggcccgctcc
    13801 cagtcgcctc ggataaaggc ctccactgct tgttcacaaa ggtggaggtg gtgctccttt
    13861 aagagaggat attcgcactc cgggggcagc agctcgtgga cgaggaccgc ctgatccaaa
    13921 ccgtatggcc gaatccgcgc gatgggccta atccgtccga tttctgaagt cacgagagtc
    13981 ctggcggcct tcgccgtctc atcgtccatc aaaatcggga catggatcag tttggtaatc
    14041 ccttctagac gtgcggccag attgacaggc ggtccaaaca cggtgatttt tgcgtggtcg
    14101 ggtgtgccaa tctttccagc cacaacacgc ccagtcgcca gaccaatccc aatgtggaat
    14161 cccgccagcg gatgtccagg agtctgcgcc gcctgactga agtcgtgtcg aatggcaagg
    14221 gccgcccgac aggctgcaag tacccgatcc tcccgcggca acggccaacc ccaaaagccc
    14281 atggcggcat ccccctgaaa gtcaccaatg actcccccat gctcgaggat ccgccgggtc
    14341 atcacctcga gggccgcact aatccggtcg agaaggggca atagttgatc agcctggcgt
    14401 tccgtctcgt gcgcaaatcc ccgcaaatcg caaaacaa
//
LOCUS       DAOSUT010000104        14424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2523_length_14424_cov_10.527177, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000104 DAOSUT010000000
VERSION     DAOSUT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_14424_cov_10.527177"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLD05_10345"
     CDS             <1..289
                     /locus_tag="PLD05_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77883.1"
                     /translation="SIPTFACPHPNPLREGEGSFWDGPLMGSYWYPDAELDGVCSPFQ
                     RLPESSHVAIDTLCWFHCRLPFPFSARIDKICPKERYVVPRETGSLENSQR"
     gene            349..1095
                     /locus_tag="PLD05_10350"
     CDS             349..1095
                     /locus_tag="PLD05_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOP77884.1"
                     /translation="MPRVICIANQKGGVGKTTTAINLAACLAKAGRRTLLVDLDPQCN
                     ATSGLGCQPLSHHPLVVGTPLSHAILTTSVKDLEILPGSRCFRDIESLVRDQGVYASM
                     LRNHLQTGMAGYDYVLIDCPPSVGQLTRTALAASNEVLMPIQCEYFAMEGLAQMIEVI
                     RRVMVERDRQLQFGGILLTMYDPRLELTLEVEEEVREFFGDIVFFTVIPRDVVCAEAP
                     SHGLPVIDYAPRARSTRAYVELCMEVLDSE"
     gene            1088..2101
                     /locus_tag="PLD05_10355"
     CDS             1088..2101
                     /locus_tag="PLD05_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOP77885.1"
                     /translation="MSKEKRLGRGLEALLGKLDSATQAPTGGTSAGAVSFDVPPVGLG
                     TIGTANSLSDSVPAADTQPTGVIEIPIDEIDPNPFQPRQEINSDEIVQLADSLNNHGL
                     LQPIVVRRHRGRYQLIAGERRFRAAKLAGWDKVPSRVVEADDRQLAELAIVENLQRKD
                     LNPLEKARCFQRYLEEYGVTQEELASRLSLDRSTISNFIRLLDLPEPVQQALREGRIT
                     QGHARALLSLDSEEDQIALCQQIEQEGLSVRRVEQIVQERQRATAGLTVVHPESSSRP
                     GRSSEHLAALEQEFRAALGLRVSLTHNGRGKGKLVVYFQSHEEFDLLRDRICGQEKHR
                     AAG"
     gene            2277..2351
                     /locus_tag="PLD05_10360"
     tRNA            2277..2351
                     /locus_tag="PLD05_10360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2312..2314,aa:Pro,seq:tgg)
     gene            complement(2538..6929)
                     /locus_tag="PLD05_10365"
     CDS             complement(2538..6929)
                     /locus_tag="PLD05_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77886.1"
                     /translation="MTLNYAQNMMVATRTIRWRPNWWGWGTLAVLVLAFGVGLPALHN
                     YQSQRIVERIRARAEAAEKRLAAAKAAGHEEEVGKALTDLVKNYDLYLRHRPGETAAQ
                     IALALAVAEQADRARGNAQLQWHAYRVLSRALAKVPDEPRLLEALASLAMRLQQWEEA
                     ASLWRELWQNDPQRVLAGLNLAKCQIAREKIREAIDTLRGVIERDPALLEAYVLLAKC
                     YRAPAVQDYESARVTIDDMVSRNHGSADAHAQRAAFWWWVSQTTSNPAEAAQAMDAAK
                     KDVATALEAVPEHLEALLISANIALHEGDLDKTKEILSLAEETAPEDRRVWAAHVNWA
                     RLAGDIDREETYLKRLTLEDPSRLPELAELHLSRQPPDLAAARNIIALMQRARFPAQY
                     LQFWQARLTFLQGDRVKGLSRLEGLYRSIGREDDALREWVGLALAEAYLQMGMRDSAG
                     AVLSQLRERAPDSPRVQAAWANWLIQSGQSELALYEFERILQSQYAARVRNSGEFLRN
                     LFLARLAAARGRPLDSPEWQQLDQLLQQVVQSSVIADDDKVLMRSRYLEARGQTDEAT
                     KLLHEAIQSDASPRLRLELASLLARQNRTDEALATLEAALQGAGFRSDILRMMIELAE
                     LVDENKRNETLRQVMQELEKLPAADQRPVVRAVARFHLRIGNTAEAEALWEKLARGDE
                     SDVEPPRVLLEMACQKGDFEKAKAWLEELRRIEGQSGRVTLLSAALLKVYQSMQKAVS
                     PSDRSRLLMEAKEDLAVLEKQFPYWPDVLRLKARIALLEGRLAAAIDQYRLLERRGLL
                     MREGKEELARLLLLRGQNEEARKVLTTSPLRVSDLQSVKLQAELLAREGRIPEAIKLV
                     EPLLAKSSDPLDWLWYAQFLSRAKQTENADKAFAHAIALAPNWPEAHLAQIMHLVATD
                     RKTEAENLIQAWQKRQIQDVSERRVLALGLELLGRPDQAEEIYRKTIQENPESGDLLL
                     EWAGFCLRQGRTSECRAVLERLVSAKADGLRVSDSTKLDARRNLAQLLGLSPDYRDVC
                     RAIELLTANLNEQGLLVDRCLLGRVLSQRPERSSRERAVAEYQTVIREGGILSPEDRF
                     AFARALASLGSWPDARMQMLELFRPGAPIQPSHLVFFINQLIRFRSPAEEIQPHLNTL
                     RSVPGYDFVALELQTKLAVRDGRADEVHGPWDERLERAIGEKRWADAEAVLTAAESSG
                     LTAWTEKAWTRLSEVRKESVLGKALFLGRQKRLKEALELCEKTSETLPITAVASVAMS
                     VLRLNRSGIQDRDLAMVEGWLLKARESNPNDKRIAIDLASFLNLAGRYEECEKAYREL
                     LARKDLSDMEKALIKNNLAYILAAKGRSVSGAASEGRLQEAESLIEQAIAVIGPIGSL
                     LDTRTVVRLGLGKNREALSDAQQAVVEEPTPLSYFHLVEALIAVGDTPTALREWQRAC
                     KDFGLTPEALPPVERERFRIVAQRLEG"
     gene            complement(7270..9075)
                     /locus_tag="PLD05_10370"
     CDS             complement(7270..9075)
                     /locus_tag="PLD05_10370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOP77887.1"
                     /translation="MLESVATGGASSAKEEKYSLLLEVAFIIFAVVLSLLGIESLPII
                     GEEPRRAEVAREMLATNDWLIPREQGEIYCSRPPLQNWLIAVIAIIRGGFDAVTVRLP
                     SALATCATALVCFAFARHHLGRMAAITSGVAFLTMGQTLTIGQMGETDALFAFFVGSA
                     LLIWYHGYATPDKRTLFWALAGLLTGLGALTKALQAPVYYLMCTVTFLLIEKNYRLIL
                     SRAYVLSYIIILVVIAAWTVPYYLAVGPQYTWQIWCGQVESRLATEGLFGHILKYPLE
                     TFVCMLPWSLFFLAFLDHRLRKSLTNWQPLVRYLVVSLLITFPTVWFIPEAKNRYFLP
                     LYPLAAVLASVPWQYWVTGGTSERVIVPRLVWRLLQAFSAMILIALLAVWPIWLGAVK
                     IELITQSTLSLDKLFIVTMLATLMVIFIHAAIRINDSRSWMCVLLSLGITLGHGHRLI
                     IVPIQCGILYSPEPAIKQLAWDVPNPERLVSIGPIVARFRYFYPHLVKLVKEEEITQG
                     PPSGVDHFCLEGEWVRRARPRKKETDRPRMAIAYRGGRVIVPKLPFAWDVVAIIPLGR
                     TLTRDPQPVAVIGRIRQGGALLSSPLRVEDGKEIE"
     gene            complement(9425..11815)
                     /locus_tag="PLD05_10375"
     CDS             complement(9425..11815)
                     /locus_tag="PLD05_10375"
                     /inference="COORDINATES: protein motif:HMM:NF039401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6288 domain-containing protein"
                     /protein_id="HOP77888.1"
                     /translation="MFVVTFTRPIVVLVMLSLATAALSADIPDFTRGDRIPEGAVHDW
                     NLGPTGARGWMYCDKLVTSDARQILITKVEKGSPADGVLAVGDVILGVGGKRFSYDPR
                     TEFGKAITLAESEAGGGRLSLIRWRAGETKEVVIKLPVLGTYSPTAPYNCPKSRIILE
                     RGCAALARRIADPNYRPDPIPRSLNALALLASGNPEYLELVKMEAHRAARFKTDGFQS
                     WYYGYLMILLAEYKLATGDDSVMPGLTRLALETANGQSKVGSWGHKFARPDGGLDGYG
                     MMNSPGIPLTIGLILARKAGVDDPKVSEAIEKSARLLRFYVGKGAVPYGDHHPWIETH
                     EDNGKCGMAAVMFNLLGERNSAEFFSRMSVASHGSERDCGHTGNFFNMLWAMPGVSLS
                     GPNATGAWMQEFGAWYFDLARRWDGTFVHQGPPEDGHDSYANWDATGAYLLAYAAPLK
                     NLYLTGKQPSCVPELDSESAWALIMDGRGWSNKDRNSFYDSLSEEELLRRLESWSPVV
                     RERAAMALGRRKPKSIAPLLEMLRSPRLEARYGACQALACLRGAAAPAVPVLIEMLDH
                     EDLWLRVKAADALAAIGRPAAMPAVPKLLQRLAKGPSAEDPRGMEQRYLCFALFDRRT
                     GLLGRSLEGVDRQALYAAVREGLRNEDGRARGAIASVYQNLSYEEVKPLLPEIYRAVV
                     ELAPSDEMFGDGIRLAGLKVLAQHRIREGMSLCLDIMELDRWGKQDRVMGGLEALSLY
                     GAAAKPLLPRLRQLEKELLAHWEARSFQPAVERLRALIAKIENSNETVTLRSLQDP"
     gene            12333..13307
                     /locus_tag="PLD05_10380"
     CDS             12333..13307
                     /locus_tag="PLD05_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77889.1"
                     /translation="MMARFRSSVLGNDAVQATGSPAGLVVAIVALVLFGGCGTTKWTE
                     TKRTATEQLLISDSMDQAVSRIDFRALAGKTVFLDDTFLKDVVDAQYLVSSLRQQLLA
                     HGAILKQKREEADYVVEVRSGAVGTDMHSLLVGVPQINLPSVAAAAGLPSSFPEIPLI
                     KRTKQTAVTKILLFAYNRETGRPLWQSGTVLAKSDAQDIWIFFAGPFQRGSIYEGTQL
                     AGDQLPISVPLIDPKEPRTAARLVEEAYYIEEPDPKSTRSRLTSKTETASPPDQRTDH
                     ATNLSHGQTAPASETTPPPSWSTTQMAFPGPVFPPDGPALGSPYAPQR"
     gene            complement(13416..14420)
                     /gene="larE"
                     /locus_tag="PLD05_10385"
     CDS             complement(13416..14420)
                     /gene="larE"
                     /locus_tag="PLD05_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOP77890.1"
                     /translation="MSTVVDNYLSVVDNMILRLFWGGIIEVKYGLYEKLSLISMESSM
                     ESPQIAFGGLSQELERKTERLLELLRSFGSCLVAFSGGLDSTVVAKAAYLALGDAAIA
                     VTGISPSMASSELEECQRLAQLIGIRHELIETQELANPLYQRNLGDRCYFCKSELFGR
                     LTDLASRFNVAVVVDGANEDDHGDHRPGLTAAREKNVRSPLAECHFKKSDVRELARFW
                     GLPNWDKPASPCLSSRIAYGEEVTAERLAMIEKAERFLRDCGLRLLRVRYHRGDLARI
                     EVPLEELPRLVSPEFRERLVTEFSQIGFKFVTLDLGGFRSGSLNTLLPPETLQKFKRS
                     "
BASE COUNT         3201 a         4312 c         3813 g         3098 t
ORIGIN      
        1 atcgattccg acgttcgctt gccctcaccc taaccctctc cgggagggag aggggagttt
       61 ttgggatggg cccttaatgg gttcttactg gtatccggat gcagaactgg atggcgtctg
      121 cagccccttc cagcgattac cagaaagctc acacgtagct attgacaccc tttgctggtt
      181 ccactgccga ttgccgttcc ctttttccgc caggattgat aaaatctgcc caaaagagcg
      241 atacgtcgtt ccacgtgaaa caggcagttt ggaaaactcg cagcgatagg atcgcttgtt
      301 gcaacggatg gcaaacgccc ttcatggggc cggcacggag gcgcgggcat gccgcgggtc
      361 atttgcattg ccaatcagaa aggaggcgtg ggaaaaacaa cgaccgcgat caatttggct
      421 gcttgtttgg ccaaggcagg tcgtcgaacc ctcctcgtcg atctcgaccc gcagtgcaat
      481 gccacatccg gtttagggtg tcagccgctt tcccatcatc ccctcgtggt gggaacgcca
      541 cttagccatg ctatcctaac cacaagcgtc aaagacttag aaatcctgcc gggcagccgt
      601 tgttttcgag acatcgagtc tcttgtccgc gatcaagggg tctatgcctc gatgcttcgt
      661 aatcacctac agacaggaat ggccggctat gattacgtcc tcatcgattg tcccccgtcc
      721 gtcggacaac tgacgcgcac cgcgttggca gcatccaacg aagtgctcat gcccattcaa
      781 tgcgaatact tcgccatgga aggcctcgct caaatgattg aggtcatccg gcgcgttatg
      841 gtcgaacgcg atcgtcaact ccagtttggg ggcatcctgc tgaccatgta cgatccgcgg
      901 ttggagttga ctctggaggt cgaggaagag gtccgcgaat tcttcggaga catcgtcttt
      961 ttcacggtga ttccgcgcga cgtcgtgtgc gcggaagcac ccagccatgg tttacccgtc
     1021 atcgattacg caccaagggc acgaagcacc cgtgcctatg tcgaactttg catggaggtc
     1081 ttggatagtg agtaaggaaa aaagacttgg tcgtggtttg gaagccttat tgggtaagtt
     1141 ggatagcgct actcaggccc caaccggagg tacgtccgca ggggccgtga gtttcgacgt
     1201 cccgcctgtg ggtctcggta caataggtac agcaaattcc ctgtctgaca gtgttcccgc
     1261 ggcagatacc cagccgactg gcgtcatcga aatccccatt gatgagatcg atcccaatcc
     1321 gtttcagccc cggcaggaaa tcaattccga tgaaatcgtt caactggctg atagcctcaa
     1381 taaccacggg ctacttcagc cgatcgtcgt gcggcggcat cggggccgct atcagttaat
     1441 tgccggggaa cgccgcttcc gcgcggcgaa acttgccggt tgggacaagg tcccgtcgcg
     1501 ggttgtcgag gccgacgatc gacagcttgc cgagcttgcc attgtggaga acttgcagcg
     1561 aaaagacctc aacccgttgg aaaaggcccg ttgtttccag cgatacctgg aagaatacgg
     1621 cgtaacccag gaagagttgg ccagccgact gagtctggat cgttcgacca tctccaattt
     1681 catccgcctt ctcgatctac cggagcctgt ccaacaggcc ttgcgagagg gacgaatcac
     1741 ccagggacat gctcgcgcgt tgctctcgct cgattctgag gaggaccaaa tcgccctttg
     1801 ccagcaaatc gaacaggaag gcctcagcgt ccggcgagtc gagcaaatcg tccaagaaag
     1861 gcagcgagca acggcaggcc tgaccgtcgt ccaccccgag tcatcatccc gcccagggcg
     1921 atcgtccgaa cacctggccg ctttggagca agagttccgt gcggccttgg gattgcgggt
     1981 ctcacttacc cataacggac gcggaaaagg caagttggtc gtctatttcc aatcacatga
     2041 agagttcgat cttttgcgag accgaatttg cggacaagag aaacaccggg cagccggctg
     2101 aaccgatgtc ccgccgccgg agaaagcccc actccagcgg ggccgcgctt tcaagtgccg
     2161 tcctccgcgc aaatgcgacg agtcacccag caaggtcacg ggagtggcag ggcggcctta
     2221 acaatcggcc ggtgggatgg taagatacgg atgcatgagg cctttttggc aagatgcggg
     2281 gcgtagcgca gcctggctag cgcatctgct ttgggagcag agggtcgccg gttcgaatcc
     2341 ggccgccccg acttactttt gagctcattc gatcggcccg ctgaggaggt caattgggca
     2401 gcgcttggtg tttgtcctca ttcgggtttt ccgcagttcg gagcgtctcg ttccatcaat
     2461 ttttggggta ggcttttgcc aaaacttttg cgcaacttgc cccattttct gcacctaccc
     2521 gagccccttt gataggttca tccttctaag cgttgagcga cgatacggaa cctttcccgc
     2581 tcaaccgggg ggagtgcctc cggcgtcagg ccaaaatctt tgcacgcccg ctgccattcc
     2641 ctcagggccg taggtgtatc gcccaccgcg atgagcgcct ctaccaggtg gaagtagctt
     2701 aacggtgttg gctcctcgac cacggcctgt tgggcatcgg aaagggcttc tcgattttta
     2761 ccaaggccta ggcggaccac cgtccgtgta tcgagcaggc tcccgatcgg gccgatgaca
     2821 gcgatggcct gctcgattag actctcggcc tcttgaaggc gaccttcgct tgctgccccg
     2881 gacacactgc gccccttggc ggccaaaatg tatgcgagat tgtttttgat aagggctttt
     2941 tccatgtccg aaaggtcctt ccgggccaaa agttctcggt aagctttctc gcattcttca
     3001 taccgcccgg cgaggttcaa aaaactggcc aagtcgatgg caattcgctt gtcattcggg
     3061 ttcgattccc tggccttgag taaccagcct tcgaccatcg ctaggtcacg atcttgaatg
     3121 cctgatcggt tcaaccgcag cacactcatg gcgaccgagg ccaccgccgt tattggcagc
     3181 gtctccgacg ttttctcaca aagctctagg gcttctttca atcgtttctg acgccccaaa
     3241 aagagggctt ttccgagaac ggattctttt cggacctcgc tcaatcgcgt ccacgccttc
     3301 tcggtccaag cggtcaagcc gctcgattct gccgcagtga gaacggcttc cgcatcggcc
     3361 caacgctttt cgccgatcgc acgttcgagt ctttcatccc acggcccgtg gacttcgtca
     3421 gctctcccat cgcggacggc caatttcgtc tgtaattcca gggcgacgaa atcgtagcct
     3481 ggcaccgacc ggagtgtatt caagtgcggt tgaatctcct ccgccggaga cctaaatctg
     3541 atcaattgat tgatgaagaa gacaaggtgc gacggctgta tcggcgcccc aggcctgaaa
     3601 agttccagca tttgcatccg cgcatcgggc caactcccca aactggccag ggcccgggca
     3661 aaggcgaatc ggtcctcggg cgagaggatc cccccttcgc gaatcacggt ctggtattcc
     3721 gccaccgccc gctctctgga agaacgttcc ggccgctgcg ataagacccg tcctaatagg
     3781 caacgatcaa cgagaagccc ttgttcattg aggtttgccg tgaggagctc gatcgcccgg
     3841 cagacatcac ggtaatccgg cgacagcccg aggagctgag ctagattccg ccgcgcatca
     3901 agctttgtcg aatcactgac gcgcagccca tctgcctttg ccgaaacaag ccgttccaaa
     3961 accgcacgac attcagaagt tcgcccctgc cgcaggcaaa agcccgccca ttcgaggagg
     4021 agatcaccac tttccggatt ttcctgaatt gtttttcggt aaatctcttc cgcttggtcc
     4081 ggccgaccta acagttccaa ccccaacgcc agaacccgtc gctccgagac atcctgaatc
     4141 tgccgcttct gccacgcctg gatgagattc tccgcctcgg tcttacgatc ggttgccaca
     4201 agatgcatga tctgggcaag gtgcgcctcg ggccaattcg gagccagggc gatcgcgtgt
     4261 gcaaaagctt tgtcggcatt ttccgtttgc ttcgcacggg ataagaattg agcgtaccag
     4321 agccaatcca gtggatcgga cgacttcgcc aatagcggtt cgacaagctt gatggcttcc
     4381 gggattcgcc cctcgcgtgc caaaagttcg gcctggagct tcaccgactg gagatcgcta
     4441 actcgcaaag gtgaggttgt aagaaccttt cgagcttcct cattctgtcc tcggagcaaa
     4501 agaaggcgag ccagttcttc cttgccctcg cgcattagga ggccacgacg ttccagcaac
     4561 cgatattgat caatcgccgc cgcgagcctt ccctccagta atgcgatgcg ggcttttaac
     4621 cgtaacacat cgggccagta cgggaattgc ttttccagta ctgcgaggtc ttcttttgcc
     4681 tccatgagaa gccttgaccg atctgaaggg gacaccgcct tttgcattga ttgatatact
     4741 ttcagaagtg cggcactcag gagcgttact cgcccacttt gcccctcgat ccgtcggagc
     4801 tcttccagcc aagccttcgc cttctcgaaa tcgcccttct gacaagccat ctccaaaagc
     4861 actcgcggag gttcaacgtc gctttcatct ccgcgtgcca acttttccca aagtgcctca
     4921 gcctccgcgg tgttaccaat tctcagatga aaacgggcca ctgcacgcac cacgggtctt
     4981 tggtccgcag cgggaagctt ttcaagttcc tgcattacct gcctgagagt ttcgtttcgc
     5041 ttgttctcat cgacgagttc cgccaactcg atcatcatcc gtaggatgtc acttcggaaa
     5101 cccgccccct gaagtgccgc ctcaagtgtg gcaagggcct catcggtgcg attctgtcga
     5161 gcaagcagcg aggccaactc cagacggagg cgaggactcg catccgattg tattgcctcg
     5221 tgaaggagct tggttgcctc gtccgtttgc ccacgggctt ccaggtaccg actcctcatc
     5281 agcaccttgt catcgtcggc tatcaccgaa gactgcacga cctgctgcaa gagttgatca
     5341 agttgctgcc attcgggact gtccaagggc ctcccccgag cagcggcaag ccttgccaaa
     5401 aagaggtttc tcaaaaactc tcccgagttc cgcactctgg cggcatattg ggattgcaaa
     5461 atgcgttcga actcatacag ggccaactcc gattgtcccg attggataag ccaattagcc
     5521 caggcggcct gcacccgcgg ggagtcaggt gcacgttccc gcaactggct aaggactgca
     5581 ccggcggagt cgcgcatccc catttggagg taagcctcgg caagggccaa gcccacccat
     5641 tcccgcaaag catcatcctc ccgaccaatg ctgcgataga gcccttcaag gcggctcagg
     5701 cctttcaccc gatcaccttg aaggaatgtc agccgtgctt gccaaaattg caagtactgc
     5761 gcgggaaaac gcgcccgctg cattagcgcg ataatgttac gagccgctgc aagatcgggc
     5821 ggctggcgac tcaaatggag ctcggccagc tcgggcaatc gcgaagggtc ctccaacgtt
     5881 agccgcttca agtaggtctc ttctcggtca atgtctcccg ccaagcgtgc ccaattgaca
     5941 tgggctgccc aaactcggcg atcctcaggc gccgtctctt ccgccaggga aaggatttct
     6001 ttggtcttgt cgagatcccc ctcgtgcaaa gcgatgtttg cagagatcaa cagggcttca
     6061 agatgttcgg gaacagcctc gagggccgtc gctacgtctt tcttggcggc gtccatggct
     6121 tgggcggctt cggctgggtt actcgtcgtt tggcttaccc accaccagaa agcggctctt
     6181 tgggcgtggg catctgccga gccatgattg cggctaacca tgtcatcaat ggtgacgcga
     6241 gccgattcgt aatcctgcac cgcgggagca cgatagcact tggccaacag aacatacgcc
     6301 tcaagcaatg ccggatcgcg ctcgataaca ccgcggagtg tatcgatggc ctctcggatt
     6361 ttctcgcggg caatttgaca cttggcaagg ttcagccccg ccaatactcg ttgcggatcg
     6421 ttttgccata attcgcgcca taacgaggcc gcctcttccc actgttgcag tcgcatggcc
     6481 agcgaggcca gggcttctaa gagtcgaggc tcatcgggga ccttggccaa tgcccggctg
     6541 aggacacggt aggcgtgcca ttgcagttga gcatttcctc ttgcccgatc cgcctgttcc
     6601 gccacggcca aggccaaggc gatttgagct gctgtttcgc cgggtcgatg ccgaaggtac
     6661 aggtcgtagt tcttaacaag atcggtgagg gccttgccaa cttcctcctc gtgaccagcc
     6721 gccttggccg cagctaaccg tttttcggct gcctcggcac gggcgcgaat ccgctcgacg
     6781 atccgctgcg actgatagtt gtggagggcc ggcaagccca caccaaacgc cagcacgagg
     6841 accgcaaggg tcccccagcc ccaccagttc ggccgccacc ggattgttcg cgttgccacc
     6901 atcatgtttt gggcatagtt tagagtcact ttactctaac cattttccta aacttggcca
     6961 tatctccata gttctcattt tacccacaat accaagacac cctctttcct cacaggcacg
     7021 caagtcccgc aagctttgca gatcacctcg cggggacaac gggaaacctt ttttccatgg
     7081 gatttccatg ggaaatatga gcgcccccag cgcgtaccat gagtatagac cggcggcaac
     7141 cctggaaaaa gtgataagtg gtttgttaga cccgagttcc ttgcgggaaa accggcttct
     7201 aatgccagag ctcggtcgcg ttttggcaat ggccgtcccg aaccagtgca atgtttgctc
     7261 tgattgggac tactcaatct ccttgccgtc ctcgacccgc agtggagacg acaacagcgc
     7321 tcccccctgc cgaatcctgc caatgacggc caccggttgt gggtcacgtg tcaacgtccg
     7381 ccccaagggg atgatggcta ccacgtccca ggcgaacggg agtttcggaa caatcacgcg
     7441 gccaccgcgg tatgcaatcg ccatccgggg ccggtccgtc tccttctttc tcggccgtgc
     7501 tctgcggacc cactcaccct ccaaacaaaa atgatccacc cctgatggtg gaccctgagt
     7561 gatttcttcc tctttcacga gcttcaccag atggggataa aagtaacgaa atcgggccac
     7621 aatgggacca atactgacca ggcgctctgg attgggaaca tcccacgcga gttgcttgat
     7681 agcaggctcc ggcgaatata agattccaca ttggatcggt acgatgataa gacgatgtcc
     7741 gtgaccgagc gtgataccca aagacaatag aacacacatc cacgaccggc tatcatttat
     7801 ccgtatagct gcatgaataa atattaccat taaagtcgcc agcattgtga ctataaacag
     7861 tttatcaagg ctaagggtac tttgggtgat taattcgatc tttacggcgc cgagccaaat
     7921 cggccacact gccagaagcg caatcaggat catggctgag aacgcttgga gtaatcgcca
     7981 gactagccgc ggtacgatta ccctctcgga tgtgcctccc gtcacccagt attgccacgg
     8041 aactgaagca agcactgcgg ccaagggata aagcggcaga aaatagcggt tcttcgcctc
     8101 tggaatgaac caaaccgtgg gaaaggttat gagcaaactg acaaccaggt aacgaaccaa
     8161 gggctgccag ttggtcaagg acttccgtaa tctgtgatcc aagaacgcca gaaagaatag
     8221 cgaccacggc aacatgcaca cgaatgtttc taagggatat ttcaggatgt ggccgaagag
     8281 cccctccgtg gcaagtcggc tttctacctg accacaccaa atttgccagg tatattgtgg
     8341 tcccaccgca agataatagg ggactgtcca ggcggcaata actaccaaaa tgattatata
     8401 ggacagaaca tacgcccgcg ataaaatgag tcgatagttc ttctcgatca gaagaaacgt
     8461 gacagtacac attaagtaat atactggagc ttgaagggcc tttgtcagcg cacctaggcc
     8521 cgtaagcaac ccagccaaag cccagaacaa cgtccgtttg tcaggtgtgg cgtagccgtg
     8581 gtaccagatc aaaagcgcgg agccaacgaa aaaagcaaag agggcatcgg tctcgcccat
     8641 ttggccaatc gtcagtgtct gacccatcgt cagaaaagcc acacccgacg ttatagcggc
     8701 cattcggcca agatgatgtc gggcaaaggc gaagcacacc aaggcggtcg cacatgtcgc
     8761 aagcgcgctc ggcagtcgca cggtgaccgc gtcgaatccc ccgcgaatga tggcgatgac
     8821 cgcaataagc caattctgaa gcggtggccg agagcaataa atctcccctt gttcgcgagg
     8881 gatcagccag tcatttgtcg ccagcatttc gcgcgcgacc tcggcccgcc gaggctcttc
     8941 acctatgatg ggaagagact cgatccctaa taacgaaaga acaaccgcga agatgataaa
     9001 ggctacttcg aggaggaggg aatacttttc ttccttcgcc gaagaagctc ctcccgtggc
     9061 aacactttcc aacatacggc tgaccaacct ctcgccgaga tgatcatgaa cctccctttt
     9121 ccttctttca ccgggcagcg cctgaccgcc ccgcgtctcg aattatatcg ttggcccctt
     9181 tttcgcttaa acaccgattg ggccgttctt ggagggtaca gccggcgcgg gggccgtgag
     9241 ccggcagagg aagacatttc gctcgaatcc gctcacggcg cgtgcaccgt ttcggacaag
     9301 gaatggcggg taaaaggacc gcctagcact ctcttatcct ttgatcgaca gcatactacc
     9361 tgccggattt ctcggcgaaa gacttgtgaa agtcccgcga aaacggcaac ggttcggagt
     9421 tagctcacgg gtcttgcagg ctacgcaggg tgacagtctc gttgctgttt tcgattttcg
     9481 cgataagcgc tcggagtcgt tccacggccg gctgaaaact gcgagcctcc cagtgagcca
     9541 gcagttcttt ctcgagttgg cgcaatcgcg gtagcagcgg cttggcagcc gccccataga
     9601 ggctgagagc ttctaggcca cccatgactc gatcttgctt tccccagcgg tcaagctcca
     9661 tgatatctag gcaaagcgac atgccctcgc ggatacggtg ttgagcaagc actttcagcc
     9721 cggccaagcg aattccatca ccgaacattt catcgcttgg tgctagctcg acaacagcgc
     9781 gataaatctc gggcaagaga ggcttcactt cctcgtaaga gagattctgg taaacggagg
     9841 cgatcgcacc tcgggcccga ccatcttcat tgcgaagccc ttcgcgaacc gcagcataga
     9901 gggcttgccg atcgacccct tctaacgaac gtcccagcaa tcccgttcgg cgatcaaata
     9961 gggcaaaaca caagtagcgc tgttccatgc cccgaggatc ctcggcagaa gggcctttgg
    10021 caaggcgctg caacagcttt ggcactgcgg gcatcgcagc aggacggcca atagcagcca
    10081 gggcatccgc ggccttaaca cggagccaaa ggtcctcatg atcgagcatt tcgatgagca
    10141 ccggtacggc cggagccgcg gcaccgcgga gacaggccaa cgcctgacac gccccgtatc
    10201 gcgcctccag ccgcggtgag cggagcattt ctaacaacgg ggcaatcgat tttggcttcc
    10261 gtcggcccag cgccatggct gcccgttcgc ggaccacagg cgaccaactt tccagccgcc
    10321 gcaaaagttc ctcttcgctc aacgagtcat aaaagctatt gcggtccttg ttggaccagc
    10381 ctcgaccgtc catgatcaag gcccaggccg actcggaatc gagttcgggc acacacgacg
    10441 gttgcttgcc cgtcagataa agattcttca aaggtgcagc ataggcgagg agataggctc
    10501 ctgtcgcatc ccaattggca tagctatcat gaccatcttc cggggggcct tgatggacaa
    10561 acgtcccatc ccagcggcgg gccaaatcga agtaccaagc cccgaattcc tgcatccaag
    10621 cgccagtggc attaggacct gaaagagaca caccgggcat tgcccaaagc atattaaaaa
    10681 agttgcctgt gtgcccacaa tctcgttccg atccatgcga ggccacgctc attcgcgaga
    10741 aaaactcggc gctgttgcgt tctcctagca ggttgaacat gaccgccgcc ataccgcatt
    10801 taccgttgtc ttcgtgcgtt tcgatccaag ggtgatggtc gccataaggg acggcacctt
    10861 ttcccacgta gaaccgaagt agccgagcgc ttttctcgat tgcttcgctg actttgggat
    10921 catccacgcc agctttgcgg gccaagataa ggccaatcgt aaggggaatg ccgggcgaat
    10981 tcatcatccc gtaaccgtcg agtccgccat ccggcctggc aaatttgtgc ccccatgaac
    11041 ccaccttgct ttgtccgttc gctgtttcca gggcaagacg ggtgagcccc ggcatcaccg
    11101 aatcgtcccc cgtcgccagc ttgtattcag cgagcaggat catcaggtag ccgtaatacc
    11161 aactttggaa accatctgtt ttaaacctgg ccgccctgtg agcctccatt ttgaccaact
    11221 caagatattc aggattccca cttgccaaaa gggccaacgc gttgagactt cgcggaatgg
    11281 ggtccgggcg ataattggga tcggcgatcc ggcgagcaag cgccgcgcaa cctcgttcga
    11341 ggatgattcg agacttggga cagttgtacg gcgctgtggg gctgtatgtt ccgagcactg
    11401 gcagcttgat caccacctcc ttcgtttccc cggcacgcca gcggatcagt gataaccgac
    11461 cgccccctgc ttcagattct gccagcgtga tcgcctttcc gaattccgtg cggggatcat
    11521 acgaaaatcg cttgccgccc actccaagga tcacgtctcc caccgccaag acgccatcag
    11581 ccggagaacc cttctcaacc ttggtaatga gaatctgccg agcgtcgctg gtcactagtt
    11641 tgtcacaata catccacccc cgggcaccag ttgggccaag attccaatcg tgtacggccc
    11701 cttccggaat tcggtcacct cgcgtgaaat cgggtatatc tgcggatagg gccgccgttg
    11761 ccaaggacag catgaccaat acgacgatcg gccttgtaaa cgttaccaca aacattgcct
    11821 agctccgttg ttctttggtg aaaggatctt ggccaacgaa tcgcccctaa caccgtggat
    11881 agcgccgctt ggacaacaga ggcaggttgc caccccaatt ctggatacgc cgctttttgc
    11941 agagtgcgcc gaatctttga tgttctccat agattaacac aaaatcgcaa tcgatcctgg
    12001 cagatgaccg tgtgacttga aacttttttg caaggcattc aatgtacgtc ggatcgatca
    12061 tacgatcgcc cccttcgctc gggggcgaga ggaaatggta catctatcca atcctcctac
    12121 ggcacgccga caagcccgcc aacctgaggg gaccacccca cgagcgattc accccctcct
    12181 acgtctttcc cccgatttat cacccgcggc aaatcgtctt ttcccgacga aaccgtttgg
    12241 ctatacttcc ccggctgtaa ttaggcaaaa aacggaggca acgggatatt ccgacacgtt
    12301 aatcgtgatc cgaaggatgg catggacggc ccatgatggc acgttttcgc agcagtgttc
    12361 ttggtaatga tgctgttcag gccacgggct cgcctgccgg tttggttgtg gccatcgtag
    12421 cgctggtcct ctttggtggg tgtggcacga ccaaatggac ggagaccaag cggacggcga
    12481 cggagcaact tttgatctcc gattccatgg atcaggccgt ctcgcggatc gatttccgcg
    12541 ccttggccgg taagacagtt tttttggacg acacgttcct taaggacgta gttgatgctc
    12601 agtacttggt cagttcgcta cggcaacaac tgttggcgca cggggccatc ctcaagcaaa
    12661 agcgagagga ggccgactac gtggtcgagg tccgatcggg cgccgtggga acggacatgc
    12721 acagcctctt ggtaggggtt ccgcaaatca atttgccctc agtggcggcc gccgcgggac
    12781 ttccttcgag cttcccagag attcccctta tcaaacggac caagcaaacg gctgtcacga
    12841 aaattctgct atttgcctac aatcgagaaa ccgggcgacc gttgtggcaa tccggcactg
    12901 tcctcgccaa gagcgatgcc caggatattt ggattttctt cgctgggccg ttccaaaggg
    12961 gttccatcta cgagggaaca caactcgccg gggaccagtt accgattagt gttcccctga
    13021 tcgatccgaa agagccgcgg acggccgccc gccttgtgga agaggcctat tacatcgaag
    13081 aacctgaccc taagtcaacg cgatcaaggc tcaccagcaa aacagaaacg gcgtccccgc
    13141 cggaccagcg aacggatcac gccacaaacc tttcccacgg acagacggca cctgcgtccg
    13201 aaacgactcc gccgccgtcg tggagtacga cccaaatggc ctttcccggt cccgtctttc
    13261 cgcctgatgg ccctgccctt ggatcgccct atgccccgca gcgatgattg cccagggtcg
    13321 gggcaatcat tgcaaagaga cataaaatca aatccttgac aggggttgca cgctcaacca
    13381 agaaacaccc caaaaagaaa ttttatcgca agctgtcacg agcgcttgaa tttctgcaat
    13441 gtctccgggg gaagtagcgt attgaggctc cccgagcgaa aaccacccaa atccagggtg
    13501 acaaacttga agccgatttg ggagaattcc gtcacgagcc tttcgcgaaa ctcgggtgag
    13561 acgagtcgcg gcaattcttc taagggcacc tcaatgcgtg ccaagtcccc acggtgatag
    13621 cgaacccgca acagccggag accacagtca cgtaaaaatc gctccgcctt ttcaatcatt
    13681 gccaggcgtt ccgcggtcac ctcctccccg taggcaattc ggctactgag gcaaggggac
    13741 gccggcttgt cccaattggg caatccccag aatcgggcga gttctctaac gtcgcttttc
    13801 ttgaagtgac actccgccag agggctgcgg acgtttttct ctcgggcggc agtcaaacct
    13861 gggcgatgat caccgtgatc atcctcattt gcgccatcca ccacaacggc cacgttgaaa
    13921 cggctcgcca aatctgtcaa gcggccaaac aattcgcttt tacagaaata gcaacgatcc
    13981 cccaggttcc gttgataaag ggggttggcc aactcctgcg tttcaataag ttcatggcgg
    14041 atcccgataa gttgggcaag ccgttggcac tcttccaatt cactggaggc catacttgga
    14101 ctaatccccg tcacggcaat cgccgcgtcg cccagggcca agtatgcagc cttggcgaca
    14161 accgtgctgt ccaatccgcc cgaaaatgcg acgaggcacg acccaaaact gcggagcaac
    14221 tcaaggagcc tctccgtttt cctttctaat tcctgagaaa ggccaccgaa ggctatctgt
    14281 ggtgattcca tggagctttc catcgaaata agcgataact tttcgtataa accgtatttg
    14341 acctcgatga tccctcccca aaacaagcgg agtatcatat tgtcgacaac cgacagataa
    14401 ttgtcaacca ccgttgacat tatt
//
LOCUS       DAOSUT010000105        14375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2531_length_14375_cov_9.516061, whole genome shotgun sequence.
ACCESSION   DAOSUT010000105 DAOSUT010000000
VERSION     DAOSUT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14375
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_14375_cov_9.516061"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLD05_10390"
     CDS             complement(<1..611)
                     /locus_tag="PLD05_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77891.1"
                     /translation="MTEGLCRHAASIESKTAPSDPDATTVSETTVQVRENLTGGDTQV
                     SLAHTSACPSGPNEKQTAGATSVRDQAASRDPNLTLIASSFDWQTPPFVQQPTGVRQT
                     NFWLDLPVLTAPNEEDFRAQEATPPSPESTDVSHATFLTATAPDRAGGASHPTNQPLQ
                     AIPDPALEGRSTHATNNRRICKDDTCWLSDFLYCDPCRPKDPW"
     gene            complement(1371..2762)
                     /locus_tag="PLD05_10395"
     CDS             complement(1371..2762)
                     /locus_tag="PLD05_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77892.1"
                     /translation="MSTSRLSRRSVLKLGAAIGLPVLVSSRALGLEGQAPASERVKIG
                     IIGLGGRARAIVNESREIKEMQIVGVSDCFKPAIDRFIKDVGQDQNWVGYEDFRAMIE
                     KEKPDGVMVETTTHARAWVTIHAMQMGMDVYIEKPMCLTIREGRYMVQAARKYNRVTQ
                     VGTQQRSMPINNWASDLVKNGAVGKITTVIAPNFVGPEQWPNKPPMPMPEGGSDKWWD
                     IWTNQAVLRPYHPELHHGWERWWDYDGGGLCYGVTGWGTHSYDQIQRALGTDETGPVE
                     MVLEEPVRTEPCGKFETIPEDDPTYLEYLNRLARPVVGPRAKVRMWYANGTELRLHLN
                     GDWGPGLGAIFIGEKGKVEINRNKIATNPKELLESPDYPGPITRPETEYHIINWIECI
                     KTRNRCNADIEIGHRSTTLCYLVNIVREVGEVGFKLRWDPVTERFTNHDGANKSSYVE
                     RPRRKGYELPPLV"
     gene            complement(3095..4009)
                     /gene="ftsY"
                     /locus_tag="PLD05_10400"
     CDS             complement(3095..4009)
                     /gene="ftsY"
                     /locus_tag="PLD05_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOP77893.1"
                     /translation="MGLLDRIKQGLKKTAQLLRTDVRDLFKTQGRLVDQAFLDELFEI
                     LIRTDMGVQAAQQIVDQIGDKYRNRVIEWNQAIEEIKTILKQLLKQPESPIKFAENSP
                     TVIMVCGVNGCGKTTTIAKLANMFRSEGKQVILGAADTFRAAAVEQLSIWAQRLGVEI
                     VTGVQGSDPAAVAHRAATRALETGADICIVDTAGRLQTHQNLMRELSKIHRVLGKQIP
                     SAPHEVLLVLDATTGQNGISQAKHFRDAVNCTGLVLAKLDGTAKGGVVVAIRQQMGLP
                     VKFVGVGEKAEDLLPFDADTFVEAMFAD"
     gene            complement(4202..4639)
                     /gene="nusB"
                     /locus_tag="PLD05_10405"
     CDS             complement(4202..4639)
                     /gene="nusB"
                     /locus_tag="PLD05_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOP77894.1"
                     /translation="MTRTIPDRRNARIVVVQILYQDDVNPEHNPAWDEEWIKRRLRHP
                     ELVAFAKSLLAGVRLYQREIDDRLSAVAENWSLSRMAITDRNILRLGAYELLYTDTPP
                     RVVIDEAVELAKLFGSQQSGPFVNGILDRLYRDIMAQRGTSVE"
     gene            complement(4653..5252)
                     /gene="ribH"
                     /locus_tag="PLD05_10410"
     CDS             complement(4653..5252)
                     /gene="ribH"
                     /locus_tag="PLD05_10410"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOP77895.1"
                     /translation="MESAVGLLPVGRIFAGDKSLPSGRFAIVVSRFNETITEGLLRGA
                     VETLTAAGVDPEQIDVFWVPGAFEIPTVAKRLALSGRFLAILCLGAVIKGETTHDQHI
                     NRAVSLQLAEIGVQTGVPVLFGILTCNTFEQALARSASREIIRDKDASQKKVGNKGAE
                     CALAALEMVRLMHQIDHTPDLAIPTTPDAGRIASERVEG"
     gene            complement(5347..6852)
                     /gene="rho"
                     /locus_tag="PLD05_10415"
     CDS             complement(5347..6852)
                     /gene="rho"
                     /locus_tag="PLD05_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOP77896.1"
                     /translation="MSQSRDVKTSADFENQVGSESNHQAPSQSQSRPRQGFRRRRRSD
                     RDEPLSIAEQLVDGRFEEEENEEEAKQFEEIKKSGDTHIAELQRMSMAELIEEARKEN
                     LSDTEIAGMKRQDLIFRILKERVKMSGLMYGEGTLEILPDGFGFLRSPDYHYLACPDD
                     IYVSPSQIRRFGLRTGMTVSGQIRPPKENERYFALLRVEAVNYEDPNLAAKKIWFDDL
                     TPCHPDKRIILETTPDEIECRVVDLIAPVGFGQRGLIVSPPRAGKTILLQKMAKAVLK
                     NYPDCYVFMLLIDERPEEVTDMERQVKGRNCEVISSTFDEAPARHIQVSEMVIEKAKR
                     FVEYGKDVIIFLDSITRLARAWNAECPASGKILTGGVDANALQRPKRFFGSARKIDEG
                     GSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIVLDRRLVDKRIWPAIDINRSGTRRE
                     EMLLDPEEHRRICVLRRVLNEMNPPDAMEFLVSRLAKTKTNAEFLMSMNMR"
     assembly_gap    6954..7053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7054..7631)
                     /gene="coaE"
                     /locus_tag="PLD05_10420"
     CDS             complement(<7054..7631)
                     /gene="coaE"
                     /locus_tag="PLD05_10420"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOP77897.1"
                     /translation="MWILGLLGGICSGKTFVAQEFARYGAQVFDADRSVHELLNREDI
                     CHRIAQCFGPGALDADGRVNRGYLASLVFGSDAESARHRKWLEELLHPEVYASLQEAM
                     VKARQLGCALFVIDAPLLLEANWSTLCHRLVYIDAPDDLRYARATERGFDRAELARRE
                     TAQASLNLKKVRADYVIHNSLNWQQTQNQVAR"
     gene            complement(7712..10468)
                     /gene="polA"
                     /locus_tag="PLD05_10425"
     CDS             complement(7712..10468)
                     /gene="polA"
                     /locus_tag="PLD05_10425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOP77898.1"
                     /translation="MSDDQGQRDKPPVNGGPSLDGQTVIVVDAHSLIHQLFHAISELT
                     SPDGRPVNAVYGFVRDMIHLLKTYQPSYLICAFDAKEPSFRKELYADYKAHRPELDAD
                     LVPQIELIEEFLHALGIPVLRQPGFEADDIMATIANEVERQGGECLLVTTDKDCRQLI
                     SDRVKILNLRKNAILDHAGLLEEWGIQPEQVVDFQALVGDSSDNVPGVPKIGPKTARD
                     LLAKYGNLESILAHLDEMPKKTWSESLRAHIDQALLSRQLVTLRRDVPLDLNWQNLRL
                     RPANRALVFDLCRRLGFGKLLSELQELLPKEELTIKKAQIEYRTIVVESLEQLRQLVA
                     QWTNVSEISLDTETTDIRPRWADLVGISLAVSLEEGYYIPIRSPLGEVRLPEAQVLDI
                     LRPILTNAQIKKIGQNLKYDLVVLRSAGVKVEGVGFDCMLADYLLSPGSRSHGLDELA
                     QRVLGHQTIKIDSLIGKGKRQLTMDQVSISRIAEYAVEDAILPWHLKQRLVPELERNS
                     LLTLFSELEVPLVEILAEMEYLGVRVDERRLSELGREFESRMADLEREIYELAGRTFN
                     ISSPKQLQEVLFEQLKLPSSKRTKTGISTDSEVLEELAEEHPLPAKIIEYRQLAKLKS
                     TYIDGLREMVCPRTGRVHASFHQAVTATGRLSCSDPNLQNVPVRTEEGRAIRSAFVAP
                     EAGWVLLSADYSQIELRVLAHFSQDSGLCEAFQRDEDIHTAVAAQVFGVSMDQVTPEM
                     RRRAKTVNFGVIYGQTPFGLAKQLHITKEEAAQFIEAYFNRYPGIDRFLAQVLEDCRR
                     TGYVSTILGRKRFVTGIRPGFTRQRNLPERTAINTVIQGSAADIIKKAMLRVYKRLHE
                     ESSPARMILQVHDELVFECPEEALSRTIAIVKEEMENVMTLRVPLKVDVAWGKNWADL
                     QSVA"
     gene            complement(10654..11370)
                     /locus_tag="PLD05_10430"
     CDS             complement(10654..11370)
                     /locus_tag="PLD05_10430"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOP77899.1"
                     /translation="MFRISLATILLLGIELTNYAVVFASEEASRIFGFSGPLTSRRWS
                     NLSWPSPWSCWTAPTGNVSLRVINEPGNPGEPVLHLRAVRGSFVLYQDLRRSPFPADR
                     YPHLSWRWKAIQLPTGASAHDSSRNDQVLQVYLAFRRSDGYDILGYVWDNPARPGEGD
                     LVHHSYRVLLFGRIEGYVQILRRGPAQDWVTETRNVVEDHKQFFRSSQPRVVAIGIWC
                     DSNDTASVSEGMIGPLVFSK"
     gene            11789..13234
                     /locus_tag="PLD05_10435"
     CDS             11789..13234
                     /locus_tag="PLD05_10435"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOP77900.1"
                     /translation="MTIYHRAIGESPGLATDLYQLTMAQGYWQHGMAERRAVFQFFYR
                     RAPFKGRFLIACGTQAVLNFLQHWRFSDEDVSYLKTLVRPDGTPLFCAEFLDYLRRLV
                     FRGEVLGVPEGTIVFPNEPILRVQAPLLIAQLLETPLLNLIGFPSLVATKAARVCRAA
                     APNPVIEFGLRRAQGLEAGLMASRAAYIGGCVATSNVLAGHLFGIPVRGTQAHSWVLS
                     FDDELTAFRAYAETYPTNCILVVDTFRTLDGIKHAIAVAQGLKAKGQKLLGIRLDSGD
                     LAHLSRVARRLLDEAGLHDVMILASGDLDEYQIAQLKAQGACIDSWGVGTRLATAFDE
                     PALTLVYKLTAIQGPDGQWEDKMKISSERAKRTIPGILQVRRFYHQGKAVCDLLYDER
                     DSEKEEPIHEVVQEAYSEKRDLLITLVDDSGRYADLPDLTSIRQYAQQELASLPENLL
                     TLRAAAQFKVVLSRGVRRRQRALQQIKEPHT"
     gene            13231..>14375
                     /locus_tag="PLD05_10440"
     CDS             13231..>14375
                     /locus_tag="PLD05_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOP77901.1"
                     /translation="MSRVRVAGAAINQTPLDWSGNLTRICDAIERAREEEVSILCLPE
                     LCITGYGCEDAFHSPSLHRTALEVLHECAAATKGMLVSVGLPLFHQSAVFNCACLLAD
                     GKIVGFVPKRYLASEGLHYEPRWFRPWPTGVRDRIHIRGQWYPIGDYVFDVGGIRIGF
                     EICEDAWVPHRPGAELAARGVDIILNPSASHFSFGKHEIRKRFVVEGSRSFRVGYVYA
                     NLLGNEAGRVIYDGGVLIATAGQLLAAGPRFAFHPVVVTSASVDVEMNRMSRARSAGF
                     QPGLVLDRDVTISIPWSYPDVEPRFEHPHVASWELGPHVKEEEFTRAVMLGLFDYLRK
                     SRAQGFVVSVSGGADSSAVACLVALMIEASYNELGHEGFCQALAHIP"
BASE COUNT         3069 a         3881 c         3926 g         3399 t
ORIGIN      
        1 ttccaggggt ccttcggccg acagggatca cagtagagga agtcggaaag ccagcatgtg
       61 tcatctttgc agatacgccg attgttggtt gcgtgtgttg atcggccttc gagagccgga
      121 tcgggaatgg cttggagcgg ctgattggtc gggtggcttg ccccacctgc gcggtctggg
      181 gctgttgccg taagaaaagt ggcgtgtgaa acatcagttg actcaggact gggaggagta
      241 gcctcctgtg cccggaagtc ttcttcattt ggtgcggtta gcaccggtaa gtccaaccaa
      301 aagttggttt ggcgaactcc agtgggctgt tgcacgaatg gcggtgtttg ccaatcgaac
      361 gaactggcga tcaatgttag attagggtct cggctcgcgg cctggtcccg aaccgacgtc
      421 gcccctgcgg tctgcttttc gttcgggccg gatgggcatg cgctggtatg ggcaagcgaa
      481 acctgcgtgt cacctcccgt taagttttct cggacttgaa ccgtggtttc tgagaccgtg
      541 gttgcatcag gatcgcttgg ggcggttttc gactctatgg atgctgcatg ccggcagaga
      601 ccttccgtca cgggagaggg cccctccttg ggggcttgta ttgttattat ggtcgctgat
      661 cgatatgccc gcatcagaag gataattccc agcatccccg ttgcaacgaa aagtgcgtac
      721 ctgcggatct ttccgaccat cgcttgcctc cggccttaac cagactccat ctttcacgcg
      781 ggccctgcct tttttgcctg tgttgaaggg tcaagatgcg cgggaaagca cagggtaacc
      841 ggtatgttgt gcgggatgtt ttacaaatga ctggaatgaa gaacaaaacg gttcttccgt
      901 aaaatcgtat cgggaaagat gaatacaacg gttcacatcg tatggggcgg tttgagaccg
      961 agggtcgttc ttcggacaag catcgtaacg gttgtatcaa gtatgcggat tgtgcggggt
     1021 gcgagcaggc tgttcgctaa aacgagtagc ttgggaagac taagatttcg gcctaaaagg
     1081 ctatcccaaa gttccctctc cctctgggag agggttgggg tgagggcaac caaccgtcag
     1141 agtccatcga gaaaaccggt agcgattcgc gctaccctca cccccggccc ctctcccgcc
     1201 gaacgggaga gaggaggttg tgggataggc tctaagtcaa cacttacgag ttaccgaaac
     1261 attttgccca gtcggtaaga tcgctacatc caacaaaatt gcccagtggc gagcaccact
     1321 gggcgattcg ctgataaaga gggcgcgaat ctcgtcgagg aaattacaac tcacacgagg
     1381 ggtggcagct cgtatccctt ccgccgcggt cgctccacat acgaggattt gttcgcccca
     1441 tcgtggttgg tgaagcgctc cgtgacgggg tcccagcgaa gtttgaaacc gacttccccg
     1501 acctcgcgga cgatgttgac caagtagcaa agggttgttg atcggtggcc aatctcaatg
     1561 tcggcgttgc agcgattccg cgtcttaata cattcgatcc agttgataat gtggtactcc
     1621 gtttcgggac gcgtgatcgg gccaggataa tctggcgatt ccaatagttc cttgggattt
     1681 gtggcaatct tgttccgatt gatttccact ttgcctttct cgccgatgaa gatagcccct
     1741 aagccaggcc cccagtcgcc attgagatgg agtctcaatt ctgtgccgtt cgcataccac
     1801 atgcggacct ttgcccgtgg accgacgacg ggccgggcca aacgattcag atactccaag
     1861 taggtgggat cgtcctcggg aatagtttcg aatttaccac atggttccgt acggacaggc
     1921 tcttcaagga ccatctctac tggcccagtt tcgtcagtcc cgagcgctcg ttggatttgg
     1981 tcataggagt gggttcccca cccggtcacg ccgtaacaca gtccgccgcc atcataatcc
     2041 caccagcgct cccagccgtg gtgcagttct ggatgatagg ggcgaagcac agcctgattg
     2101 gtccagatat cccaccactt atcgcttccc ccttcgggca tgggcatggg cggcttattt
     2161 ggccactgtt ctggcccgac aaaattgggt gcaattacag tcgtaatctt acccaccgct
     2221 ccgtttttaa ccaaatcgct ggcccaatta ttgatgggca tagaccgctg ctgagttccc
     2281 acctgagtta cccgattgta cttccgcgcc gcctgcacca tgtaccgtcc ttcccggatc
     2341 gtcaagcaca tcggtttttc gatgtaaaca tccatcccca tctgcatggc atgaatggta
     2401 acccaggcac gggcgtgcgt tgtcgtttcg accatgactc cgtcgggctt ttctttttcg
     2461 atcatcgccc ggaaatcttc gtacccaacc caattctggt cctggccgac gtccttgatg
     2521 aaacgatcga tggcaggttt aaaacaatcc gagacgccaa caatctgcat ctctttgatt
     2581 tcgcgggatt cgttaacgat tgcgcgggcc cgcccgccga gtccaatgat gccaatcttg
     2641 acgcgctccg aagcaggggc ctgaccctcc aaccccaacg cacgactgga aacgaggacc
     2701 ggcagcccaa tcgcggcacc cagttttagg acagaacggc gtgacaatct agacgtggac
     2761 atggttggat tcctttcatc agcaaaagac aacgtaaact tttcggtgat tgtgaactgc
     2821 ggcgcacccc ttgtcaatca aaatcgagga aacataactt gggtcctgac cgcttggctg
     2881 atgaatagga taaccatttc ccgatgcgat tccaactggg taccgggcga ccaggggcca
     2941 aggcaggacg tgagtgctct aaccatcaag agagacaagg cccctgtgcc tgcccgatgt
     3001 gttagaagtc cacatgccat cgtagccgca tcaaactcgc ctattgaacg agaaaggttg
     3061 tgtatgagga aacggtaatg atgagagatc gcccttagtc ggcgaacatg gcctcgacga
     3121 acgtatcggc atcgaagggc aataggtcct cagccttttc cccgacgccc acgaacttga
     3181 ctgggagtcc catttgctga cggatggcca ccaccacgcc gcccttggcc gtaccgtcca
     3241 gctttgcaag caccaatcct gtgcagttca ccgcgtcgcg aaagtgtttc gcttggctga
     3301 tgccgttttg tcctgttgtt gcatcgagga caagcaggac ctcatgggga gcggagggaa
     3361 tctgcttacc cagtacgcga tgaatcttgg agagctcgcg catgaggttt tgatgtgttt
     3421 ggagccggcc tgctgtatcg acaatgcaga tgtcggcacc cgtttcgaga gcacgggtcg
     3481 ccgcacggtg tgccacggca gccggatcgc ttccctgaac cccagtcaca atttccactc
     3541 ccaggcgttg cgcccagatg ctcagttgtt ccacagccgc tgcccgaaat gtgtcagcag
     3601 cacctaagat cacctgcttc ccttcagaac ggaacatatt tgccagcttg gcaatcgtgg
     3661 ttgtcttgcc gcatccgttg acgccacaaa ccataatcac ggtcggactg ttttcggcaa
     3721 acttgatcgg ggattccggt tgtttgagga gttgcttgag tatggttttg atctcctcga
     3781 tcgcctggtt ccattcgatg acccgatttc gatatttgtc cccgatctgg tccacaattt
     3841 gttgggcggc ctgaactccc atatcggttc ggatcaagat ttcgaacaat tcatcgagaa
     3901 acgcctgatc gacgagacgc ccttgtgtct tgaataggtc gcggacgtct gtccgaagga
     3961 gttgtgccgt cttcttcagt ccctgtttga tacggtcaag taaccccata gttttcaccc
     4021 atcgcaccag cagtgatgtt cgttagccct tctcaaattc tcgcaaagct gagccagagg
     4081 tttatcgttg cagtcggcca ggaccaagtt gggcaatctt gttcgaaaac tcggatgatt
     4141 ggttcatgta taacggctgc cttgccaagt ggatcacaag atgcgtccaa aaacgagccc
     4201 atcattcaac cgaagtaccc cgttgcgcca tgatgtctcg atagagtcgg tcgaggatac
     4261 cattaacgaa tggacctgac tgctgactgc caaaaagttt tgcaagctcg acagcttcgt
     4321 caataacaac ccgcgggggc gtgtccgtat aaagaagttc gtaggccccg agacgtaaaa
     4381 tgttacgatc agtaatggcc atccgcgata acgaccaatt ctcggccacg gccgataatc
     4441 tgtcatctat ctcacgttga taaagccgaa cgccagcgag tagcgatttg gcaaaggcca
     4501 ccagttcggg atgccgcagc cgcctcttta tccactcctc atcccaagcc ggattgtgtt
     4561 cggggtttac atcatcctgg tagagaattt ggaccacgac aattcgggca tttcttcgat
     4621 ccggtatagt gcgtgtcatt ggcgggctca catcatcctt ccactctttc ggaggcgatt
     4681 cggccagcgt caggagtcgt agggattgca agatcggggg tatggtcgat ttgatgcatt
     4741 agccgcacca tttccaaggc agcgagggca cactctgccc ctttatttcc gactttcttt
     4801 tgggaggcat ctttgtctcg gatgatttct cgggaggccg atcgggcgag ggcctgctcg
     4861 aacgtattgc acgtcaagat tccaaacaag accgggacac ccgtctggac gccaatctcc
     4921 gccagttgca aactgacggc ccgattgatg tgctgatcat gagtcgtttc acctttgatg
     4981 acagctccca gacagaggat cgccaaaaaa cggccagaca gtgccaaccg tttggctacc
     5041 gtgggaattt caaaggcgcc ggggacccaa aaaacgtcga tctgctcggg atcaacacct
     5101 gctgctgtta acgtttcaac tgcccctcgc aaaagcccct ctgtgatcgt ctcgttaaag
     5161 cgggagacaa caatggcaaa ccggccagac ggtaggcttt tgtccccggc aaagatgcgt
     5221 cctaccggca gcagtccaac agcagattcc atggaatact cccgtgtccc tttcagataa
     5281 tgtggcgttt atcagccagg agcctcgtcc aggttgggcg tcaagacccg ccagcctgcc
     5341 aaacaattaa cgcatgttca tcgacatgag gaactcggcg ttcgtcttag tcttggccag
     5401 acggctgacg aggaactcca tggcgtctgg cggattcatt tcgttgagga cccgtcgcaa
     5461 gacacaaatt cggcggtgct cttcggggtc aagtaacatc tcctcgcggc gtgtgcccga
     5521 ccgattgatg tcgatcgccg gccaaattcg cttatcgaca agtcggcgat cgaggacgat
     5581 ctccatgtta ccggtccctt tgaattcctc aaaaatcacc tcatccatct tgctaccggt
     5641 gtcgatcagg gccgtggcga tgatcgtcag ggaaccgccc tcatcgatct tccgggccga
     5701 accgaagaat ctcttaggcc gttgcaaggc gttggcatcg acaccgcctg tcaaaatttt
     5761 tcccgacgcc ggacattcgg cattccatgc ccgcgccaaa cgggtgatcg aatctaggaa
     5821 gatgattaca tccttgccgt attcgacgaa tcgcttggcc ttttcgatga ccatctctga
     5881 aacttgaata tggcgggcgg gggcctcgtc aaatgtcgag ctaatcacct cgcaattgcg
     5941 gcccttgacc tgccgctcca tatcggtgac ctcttccggt cgctcgtcga tcaggagcat
     6001 aaacacgtag cagtccgggt agtttttgag aaccgccttg gccatctttt ggagcaagat
     6061 ggtctttccg gcccgtggcg ggctgacgat caggcccctc tgtccgaacc caacgggggc
     6121 aatcaagtcg acaacgcggc attcgatttc gtcgggggtg gtctccaaaa tgatgcgttt
     6181 gtcagggtga cagggcgtca agtcatcaaa ccagattttt ttagcggcca agtttgggtc
     6241 ttcgtagttc acggcctcca cccggagaag ggcaaagtac cgctcatttt ctttgggagg
     6301 ccgaatctgt cccgaaaccg tcatccccgt ccgcaagcca aaacgtcgaa tttgactggg
     6361 agaaacgtag atatcgtccg ggcaagccag gtagtgatag tctgggctgc ggaggaagcc
     6421 aaaaccatcc ggtaggattt ccagcgtccc ttcaccatac atcaggccgc tcatcttgac
     6481 ccgttccttg aggattcgga agatgaggtc ctgccgcttc attccggcaa tttctgtatc
     6541 ggagaggttt tccttgcggg cctcttcaat cagttccgcc attgacatcc gctggagctc
     6601 tgcgatgtgc gtatcgccgg acttcttgat ctcctcaaat tgtttggcct cttcttcgtt
     6661 ttcttcctcc tcgaaacgac catccacaag ttgttcggct atagaaagcg gttcgtcccg
     6721 gtcactccgg cgacggcggc gaaagccttg acgtggtcgt gattgagatt gagaaggtgc
     6781 ctggtgattg ctctcgctac cgacttgatt ctcaaaatcg gctgatgtct tgacatcacg
     6841 tgattgagac attgttgata ctcctgaaaa gtcaacgctc ctgatttgca cacgaacggt
     6901 gctgctgagg tgggatatcg ggaaagattg gacaagctcc aagcttccca aaannnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatccggg cgacctgatt ctgggtctgt
     7081 tgccaattga ggctgttatg gatcacataa tcggcgcgga cctttttgag gttcagggat
     7141 gcttgcgcgg tctcacggcg agccaattcc gcgcgatcga accccctctc cgtagcgcga
     7201 gcatacctca aatcatcggg ggcgtcaata taaaccagcc tgtgacatag tgtactccaa
     7261 ttggcctcca agagcaatgg ggcatcaata acaaacaacg cacaccccaa ttgacgcgcc
     7321 ttcaccatcg cctcctggag gctggcgtac acctcagggt ggaggagctc ctccagccac
     7381 tttctgtggc gcgcggattc tgcatccgaa ccgaaaacaa ggcttgccag gtacccccga
     7441 ttaacacgac cgtcggcatc aagcgcccca gggccgaaac actgggcaat ccggtggcaa
     7501 atgtcctctc tgttgaggag ttcatgaacg gagcggtcgg cgtcaaagac ctgagccccg
     7561 taacgcgcaa actcctgggc cacaaaagtc ttccccgaac agataccccc taaaaggcca
     7621 agaatccaca tggatcaacc catttgcgaa atagataccc ctacactgag gggatggctt
     7681 ctgccgttta ctgaagttcc ttcttgttgg tctacgcgac agattgtaga tcggcccagt
     7741 tctttcccca ggcgacatct accttcaagg gaacccgcaa agtcattacg ttttccatct
     7801 cttctttgac aattgctatg gttcgtgaca gcgcttcctc gggacactca aacacaagtt
     7861 cgtcgtgaac ctgcaaaatc atgcgggcgg gactcgattc ctcatgaagt cgcttataca
     7921 cccttagcat agccttcttg ataatgtccg ctgccgaccc ttggatcacc gtgttgatgg
     7981 cagttcgctc gggcaaattg cgttggcggg taaaaccggg gcgaatccct gtcacaaatc
     8041 gtttcctacc gaggatggtg gacacgtaac ccgtgcgccg acaatcttcc aagacctggg
     8101 cgagaaagcg atcgatcccg ggatatcggt tgaaataggc ctcgatgaac tgggctgctt
     8161 cttccttggt gatatgtaac tgctttgcca gtccaaaggg tgtttgccca tagattaccc
     8221 caaagttgac cgttttggcc cgtcgtcgca tctctggcgt gacctgatcc atagatacgc
     8281 caaacacctg cgcagccacg gccgtgtgga tatcttcatc tctttgaaat gcctcgcata
     8341 gcccgctgtc ttgggaaaaa tgggccagaa ctcgcaattc aatttgagaa taatcggccg
     8401 agaggaggac ccaacctgcc tccggagcta cgaaggctga acgaatggcc cgcccttcct
     8461 cggtgcgaac cgggacgttt tgaagattgg ggtcgctaca gctcagccta ccagtggcag
     8521 tgacggcctg atggaacgaa gcgtggaccc ggcctgtccg ggggcagacc atttctcgaa
     8581 gaccgtcgat ataagtactt ttcagctttg ctaattggcg atattcgatg atcttggcgg
     8641 gcaagggatg ttcctccgcc aactcctcga gaacttctga atcggtgcta atccctgtct
     8701 tcgtccgttt gctgcttggt agtttgagct gctcaaaaag aacctcttgg agttgcttcg
     8761 gggaagagat attgaaggtt cgaccggcga gttcatagat ttcccgttcc aagtcggcca
     8821 tccgcgattc aaactcgcgt cccaattccg aaagccgacg ctcatcgacc cggacgccga
     8881 gatattccat ttccgccagg atttccacta gcggaacctc caactcggag aacagggtta
     8941 gcaggctgtt gcgttccaat tcgggcacta gcctctgttt caagtgccag ggtaggatcg
     9001 cgtcttccac cgcgtactca gcgatccggg aaatgcttac ttggtccatc gtcaactgtc
     9061 gcttgccctt accgatgagc gaatcgatct tgatggtctg gtgaccgagc acccgctggg
     9121 caagctcgtc caggccgtgc gaacgggaac ccggcgatag gaggtagtcg gccaacatgc
     9181 agtcaaaacc aaccccttca actttcaccc cggccgatcg aagcaccacc aaatcgtatt
     9241 tgagattctg cccgatcttt ttgatttgtg cgtttgtcag gattggcctg aggatgtcga
     9301 ggacctgagc ttccgggaga cgcacttcgc ccaatggcga acgaatgggg atgtagtaac
     9361 cctcctcgag gcttacggcg agagagatgc ccaccaggtc ggcccaacgg ggacggatat
     9421 cggtcgtctc ggtatccaga cttatttccg aaacattagt ccactgggcg acgagttgac
     9481 ggagttgctc caaggactcc accacgatcg ttcggtattc gatttgcgct tttttgatcg
     9541 tcagttcttc tttgggtagc aattcctgca gttcgcttag cagttttcca aagccaagac
     9601 ggcggcacag atcaaagacc aaggctcgat tggcgggacg taagcggaga ttctgccaat
     9661 taaggtccaa aggcacatcc cgccgcaatg tgacgagttg tcgagataat aaagcctgat
     9721 ctatgtgggc acgtaggctt tccgaccagg tttttttcgg catttcgtcc agatgtgcca
     9781 gaatagattc caagttgccg tattttgcca gtaggtctct ggctgtcttg ggaccgattt
     9841 tcggaacacc cgggacgtta tcgctgctat cgcctaccag ggcttggaag tcaaccacct
     9901 gttccggctg aatcccccac tcctccaata agccggcgtg atccaggatg gcgttttttc
     9961 ggaggttgag gatcttcacg cggtcactga ttaattggcg acaatctttg tcagtggtaa
    10021 ccagaagaca ctcgccgccc tgtctctcga cctcgttcgc aatggtggcc atgatgtcgt
    10081 ccgcctcgaa ccccggttga cgcaggacgg ggattccgag ggcgtggagg aattcttcaa
    10141 tcaattcgat ttggggtact aggtcggcat caagctcggg acgatgggcc ttgtaatcgg
    10201 cataaagctc ttttcgaaag cttggctcct tggcatcaaa tgcgcaaatg aggtaacttg
    10261 gttggtacgt cttcaagagg tggatcatat cccggacaaa gccgtaaact gcattgacgg
    10321 ggcggccatc cggcgatgtt aattcgctga ttgcgtgaaa caattggtgg atcagagaat
    10381 gggcatcaac cacaatcacc gtttgaccgt ctaatgacgg tcctccgttt accggcggct
    10441 tgtcacgttg tccctggtcg tctgacatcg aaagacctcg tcgctaccaa atatgagccc
    10501 tattacgcca agagtcatgg gctggtccaa agaagcgcag caattcccat tttgggatat
    10561 ttcgacagcc ctcgccacct tcctcctgac gtaagacaac agactgactt caaaacaagg
    10621 atgacgacgg acgagatgcc ctagatctta aggctatttg gaaaacacga gtgggccgat
    10681 catgccttca gaaacactcg ccgtatcgtt ggaatcgcac cagataccga tggccacgac
    10741 tcgtggctga gagcttcgaa aaaactgttt gtgatcttct acgacgttgc gagtttcggt
    10801 gacccaatct tgggctgggc cacgccgcaa gatctggaca taaccctcga tcctgccaaa
    10861 aaggagaacg cggtatgagt ggtgaacgag gtcgccttcc ccggggcgcg cggggttgtc
    10921 ccagacatag cccaggatat cgtagccgtc gctccgtcga aaagccaaat acacctgaag
    10981 gacttggtcg ttgcgggagc tatcgtgtgc actggccccc gtgggcagtt ggatggcctt
    11041 ccaccgccaa ctgaggtggg gataacggtc agccgggaag ggagacctcc gcaggtcttg
    11101 atagaggaca aatgaaccac gaaccgcccg aaggtgcaag accggctcgc ccggattacc
    11161 tggctcgttt atcacccgga gactcacatt gccggtgggg gccgtccagc aactccaggg
    11221 tgatggccaa cttagattgc tccagcgtcg gcttgttagg ggaccagaga atccgaaaat
    11281 gcgacttgcc tcttcactgg cgaaaacaac ggcgtaattc gtcaattcga tgccgagcaa
    11341 caggatcgtg gccagagaga ttcggaacat tataggctcc cgccgccctc atcctgccag
    11401 ttgggccagt atggattcat taggtgtggt ctcggctgtg ccgaatattg ccgcttttgc
    11461 ccctcatttt agagtttcca agaccgcccg acaaccgagc ccggaatgat ccttgagttc
    11521 ttccgtcccc gtgacttgga gggacattgc gttcctataa tggacgtggt gttgctcgtg
    11581 acgatgtttg ctcgttttgg gattagccca gcaagcccgt ccattcactc tgtgtcatgg
    11641 ggttgtggct cccgaagacg acgagatgga tggtttggtt acgtcgaagg ccatttgcgg
    11701 gaacgggcgg ttggtctctg acaaagacgg aacagagaga accttttccc attacacatt
    11761 gtcggataag agttgagtgc aaactgcgat gacgatttac caccgagcca ttggagaaag
    11821 tcccggccta gcgacggatt tataccaatt gacgatggcc cagggctact ggcaacacgg
    11881 catggccgag cggagggccg tgtttcagtt cttttatcgc cgggcaccct ttaagggtcg
    11941 ttttctgatc gcctgtggta cgcaggccgt tctcaatttt ctgcaacact ggcggttttc
    12001 cgacgaggac gtttcttatc tcaagaccct tgtccgtcca gacggaacac cactgttttg
    12061 tgctgagttc ctcgattatc ttcgaaggct cgtttttcgc ggggaggttt tgggtgtacc
    12121 ggaggggacc attgtctttc cgaacgaacc catcttgcgg gttcaggccc ctttattaat
    12181 tgcccagttg ttggaaactc cccttcttaa cctgattggt tttccctcct tggttgccac
    12241 gaaggctgcc cgtgtttgcc gagccgccgc tccgaacccg gtgattgaat ttgggctacg
    12301 ccgggcgcag gggctggagg cgggactgat ggcttctcgt gccgcctaca ttgggggatg
    12361 cgtggcaacc tccaatgtcc tagctggtca tcttttcgga atacccgtgc ggggaacgca
    12421 ggcgcatagt tgggtcctta gtttcgatga tgagctgacc gcgtttcggg cgtacgcgga
    12481 gacttatccc accaactgta ttctcgtagt tgacacgttt cggacacttg acgggatcaa
    12541 acacgcgatc gcggtagccc aggggctgaa ggcgaaaggc cagaaactgt tggggattcg
    12601 cttggattcc ggtgatctgg cccatctcag tcgcgttgcc cgaagattac tcgatgaagc
    12661 aggattgcat gacgtgatga tcctggcctc cggtgatctt gacgaatacc agattgcgca
    12721 actgaaggcc caaggggcgt gtatcgactc ctggggagtg gggacgcgtc tggctacggc
    12781 gttcgacgag cccgccctta cccttgtgta caaattgaca gccattcagg gcccggatgg
    12841 ccaatgggaa gataagatga agatatcgtc cgagagggcg aaacggacca tcccaggcat
    12901 cttgcaggtc cgcaggtttt accaccaggg taaggcggtg tgtgatcttc tatatgacga
    12961 acgtgattcg gagaaggaag agccgatcca cgaagttgtc caggaagcgt acagcgaaaa
    13021 gcgagacttg ttgattacgt tggttgatga cagcggaagg tatgctgatc ttcccgatct
    13081 gacctcgatc cgtcaatatg cacagcagga attggcttcg ttgccggaaa accttcttac
    13141 cctgcgtgct gcagcgcaat tcaaggtcgt gctaagcaga ggtgtgcgtc gccggcaacg
    13201 tgccttgcag caaataaagg agccccatac atgagtcgtg tccgtgtggc tggagcggct
    13261 atcaaccaaa caccactcga ttggtctggc aatcttacga gaatctgcga tgcgatcgag
    13321 cgggcaaggg aagaggaggt gagcatcctc tgcctgcccg agctttgcat caccggttac
    13381 ggatgtgagg acgcctttca tagcccgagc ctccatcgaa cggccctcga agtccttcac
    13441 gagtgtgccg ccgccaccaa gggaatgctc gtgagtgttg ggctgccact ctttcatcag
    13501 tctgccgttt tcaactgcgc atgcctgttg gccgacggga agatcgtcgg cttcgttccc
    13561 aaacggtatc tggcgtcgga gggccttcat tacgaaccgc ggtggtttcg cccatggcct
    13621 accggggtcc gtgaccgaat ccatattagg ggacaatggt atccgattgg ggactatgtg
    13681 tttgacgtgg gtgggattcg gatcggtttt gaaatttgcg aagatgcctg ggtcccgcac
    13741 cgcccagggg cggaactggc cgcccgcggt gttgacatca tccttaatcc aagtgcgagt
    13801 catttttcct ttggcaaaca cgaaatccgg aaaagatttg ttgtggaagg ttcacggtcg
    13861 tttcgggtgg gttacgttta tgccaatctg ctcggcaacg aggcaggacg ggtcatctac
    13921 gatgggggtg tccttatcgc cacggcgggg caattgttgg cagccgggcc ccgatttgcc
    13981 ttccacccgg tggttgttac ctcagcgagc gttgacgtgg aaatgaatcg gatgagtcgg
    14041 gcgcggtcgg ccggttttca acctggcctg gtcttggatc gtgacgttac gattagtatc
    14101 ccctggtcgt atcccgacgt tgaaccgcgc tttgaacatc cccacgttgc ctcgtgggaa
    14161 ttggggccac acgtcaaaga ggaggaattt acccgtgccg tgatgcttgg gctcttcgat
    14221 tatttgcgga agagtcgggc acagggcttt gtcgtcagtg tgagtggtgg ggccgactcg
    14281 tcggccgtgg cgtgccttgt cgccctgatg atcgaggcct cctacaacga gctcggtcac
    14341 gagggttttt gccaggcact ggcgcatatc cctga
//
LOCUS       DAOSUT010000106        14366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2537_length_14366_cov_9.341695, whole genome shotgun sequence.
ACCESSION   DAOSUT010000106 DAOSUT010000000
VERSION     DAOSUT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_14366_cov_9.341695"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2271)
                     /gene="hypF"
                     /locus_tag="PLD05_10445"
     CDS             complement(<1..2271)
                     /gene="hypF"
                     /locus_tag="PLD05_10445"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOP77902.1"
                     /translation="MNKRSENGHNDTFLPPKAPPWENVEAPGDGQRWRITVSGVVQGI
                     GFRPFVYRMAHKWGLQGWVKNTADGVILEVEGQPDVLRAFWEDFWKSLPAGAAFDNYH
                     RSSVPALGGAEAAGFRIVSSDDGGERRPNIPPDLAVCRECLAELANPTDRRYRYPFIN
                     CTHCGPRWSIVEGVPYDRPLTSMKGFRLCPDCEREYRDPLDRRFHAQPIACPRCGPHL
                     ELWNRNGQIVAEKDEALRQAALAVLAGKILALKGLGGFQLICDATSGEAVRQLRIRKR
                     RPDKPFALMMTEEMIRLSCVVPSQTVWDRLRSPASPIILLPRKDAENTQDHESCPWAI
                     SESVAPGNPYWGVMLPYTPLHHLLMETVNRPIVCTSGNISEEPMAIDNAEALHRLQGI
                     ADVFLVHNRPIVRPVDDSVMATDRSGNVVMVRRARGFAPRSLSLSGHQVPILATGGHL
                     KNVVGLAIGNQAILSAHIGDLDNPLAFEAHRRAIEDLLRFFDTAPEVLSCDLHPDYAS
                     TRSAEDLARRLKIPVFRWQHHVAHFAACLAEAESDEARRAVNELALSQETTTPGHPSG
                     ELPILGVIWDGTGYGTDGTSWGGEFFLFDGTSFCRVARLRTFPLPGGDIVVRQPRRSL
                     LGLIYETGDRSLEQTLEKFFTPQELKVLLRAMERNVQSPRTSSMGRLFDGIAALLGFG
                     GHTSFEGQAAMALQYAAERGLLRAYPIISPRPFGGRGDGGEGGSNRCEFSQSIPTFLC
                     PHPNPLPEGEGDFLRQA"
     gene            2593..3684
                     /gene="lpxK"
                     /locus_tag="PLD05_10450"
     CDS             2593..3684
                     /gene="lpxK"
                     /locus_tag="PLD05_10450"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOP77903.1"
                     /translation="MFKIDQLAYLRIIAGEDRSLGALAIRSLLLPAEFIYVLAMRFRN
                     LAYELKWLKVQSLEVPVISVGNLTLGGTGKTPLVAWLARFFRRQNIRVTIISRGYGAA
                     ESSRNDEARTLERLLPDVPHLQNPDRLAAAEVAIRELATQVIILDDAFQHRRIARDLD
                     IVLIDALSPFGFGHVFPRGLLREPVAGIRRAKVVILSRADLVDELTRRRLRDEVLRIH
                     PQAQWAEVCHVPTCLVAANGEETALETLRGQRVAAFCGIGNPEGFRQTLLRLNYDLVD
                     FRIFPDHHPYTREDLEELTRWLERIKAQVAICTEKDLVKIGLDQLGQIPLWALRVEIE
                     FLSGREVLEAQLRPIVERILATPDPLFST"
     gene            complement(3702..>5345)
                     /locus_tag="PLD05_10455"
     CDS             complement(3702..>5345)
                     /locus_tag="PLD05_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77904.1"
                     /translation="IQRAMDEMSRRGGGIVEIPAGTYRIYGHLVVPQDVTLQGVLQTA
                     PTTRWAKWQEVGGSVLLAYEGRGSEEGEPFIRLAGRHASIRGLTVVYPEWKQSDVPPV
                     PYPPCVASYNTENVSIQDCLLVNPYEAIRLVRAHRHLVRNVTGYPIKRGLYVDECYDI
                     GRVENIHFWPFGVSYRPNDPYCEWINLNGVAFEFARTDWHYVFNTFCFGYGVGYKFSK
                     SSKGSANGNFLGIGADCCQRAVLVESTQPPGLLITNGEFVGRWGSKDAVTLEVVPEAT
                     GRVSLVNCSFWGPIDRCVWLRSEDVQTTLMACHFCQWDVAYQGSPAIQVDAGWVTIQG
                     CSFDQDSSVHLAISKDVRSALVVGNQAMGGLRVNNQAGNRAQIGLNAVDPVEWTEEAK
                     EHYQLEIGRPGDARYLLGWHGAEKGFRPFRWTSGSSRLLLPVIPGRGYQLTISVEIPK
                     VLDDAEITLVSSGQTLGNIQRGTTTVTLPPQSSDRVMIEIRTPTWVPQKVIPRSQDQR
                     ELGIQVFSVVMKADGAKDQVFNANIGEWLPREAPSVEQR"
     assembly_gap    5346..5445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5731..7245
                     /locus_tag="PLD05_10460"
     CDS             5731..7245
                     /locus_tag="PLD05_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOP77905.1"
                     /translation="MTVEQFVDLLKRSQLIEADQLQKLLAEYLPPEGPSSLTAEELAE
                     KLIAAGILTRWQCDKLLEGRYKGFFLGKYKLLDHLGTGGMSSVYLAEHVLMQRRVAIK
                     VLPKHRVQDASYLARFLREAKAAAALDHRNIVRAYDVDNDGVNHYLVMEYVEGQDLQR
                     MVRERGPLPYNEAAEYIRQAAEGLHHAHQAGLIHRDIKPANLLVNKNGTVKLLDLGLA
                     RFTDDERGSLTIAYDENVLGTADYLAPEQAVDSHRVDHRADIYSLGCTLYYLLTGHPP
                     FCEGTLAQRIMKHQREAPPSIRDERPDAPEDLVAICLKMMAKKPSARYQTAAEVAQAL
                     RLWLINHGYEVPESDTRLPKPVKAAPAGTTKEKAPVRTLVSAKALPDSAAVPDTKTAL
                     QTAPRETSPKTEQSSPNLGVPVAKPLEGANTLPIAKPLVETATIPHELVDQAGDEAEA
                     SPFPLPLPADEDPRLALVLGGRARRSAPPMPFWIWIIIALGLVTAVVLAIIQFV"
     gene            7392..7859
                     /locus_tag="PLD05_10465"
     CDS             7392..7859
                     /locus_tag="PLD05_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP77906.1"
                     /translation="MKERHKILIADDNPTNVELLEAYLADYDCEIAVASDGLETLAMA
                     RKIHPDLILLDIMMPKMSGFEVCQKLKSDPATKDIMILMVTALNELGDIERAVAAGCD
                     DFLSKPIHKLELLKRVENMLKLRQLTDELARLRAYIDAMEETRPGQRGPAKGY"
     gene            complement(7873..7946)
                     /locus_tag="PLD05_10470"
     tRNA            complement(7873..7946)
                     /locus_tag="PLD05_10470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7910..7912),aa:His,seq:gtg)
     gene            complement(8351..9235)
                     /locus_tag="PLD05_10475"
     CDS             complement(8351..9235)
                     /locus_tag="PLD05_10475"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOP77907.1"
                     /translation="MSSLAQTPLARSCLWLPSLFVLGLLVPLNSIQAGTVDFEDVGAS
                     LPANSYWNGAPNDGHNTFTSNGFIFHNNYTLAWDVWDGFAYSNKTDTTTRGYLNQYSA
                     ITGSGADNSATYAVSFVNLWGDLPRIEVPEGVSLVSAQVTNTTYAYYSMLEGDQFAKK
                     FGGPSGNDPDWFKLIIYGKDLANSLLGTVQFYLADFRFEDNAQDYIVNTWQTVDLTPI
                     STARILEFELDSSDVGPWGMNTPACFALDNLVFQTPGSNGEVIPEPTSVVLLGLSTVA
                     IGGVSVLRRWRRREKDQV"
     gene            complement(9308..10177)
                     /locus_tag="PLD05_10480"
     CDS             complement(9308..10177)
                     /locus_tag="PLD05_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77908.1"
                     /translation="MDRRSALLRNRCGERTLRLFSRVIRKGFTLVELLVVIAIIGLLT
                     ALLLPAVQAAREAARRSACTNNLRQMGIGLHNYHQALGCFPPGGIEHRAMINPQTKKP
                     YGPAGRQLAWSLFLLPYVEQQPLYQKVDTSKPFDAPENAEAAATILPLYICPSATTQR
                     ELRQGRGPCDYGGIYGERITSPNNPPKGMMLYDQALTVADVIDGTSNTLIVSEDSDFR
                     DGQWINGLNVFDQAYAINKAPAFENDIRSKHPGGANGLFTDASVRFLAETMDLSVLAA
                     ICTRAGGEVVTGL"
     regulatory      complement(10337..10585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10787..11893
                     /gene="ribD"
                     /locus_tag="PLD05_10485"
     CDS             10787..11893
                     /gene="ribD"
                     /locus_tag="PLD05_10485"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOP77909.1"
                     /translation="MDQADLDAWHMRRVLELAKRGQGSVEPNPMVGCVIAKGAEIIGE
                     GWHRAFGQPHAEIEALRLAGSRAAGATMYVSLEPCCHYGKTPPCTQAVIKAGIRRVVV
                     AMKDPFPLVAGKGLSELNKAGIEVSVGLMEAEARSLNAPYIKLITQQRPWVIAKWAMT
                     LDGKIATYSGDSRWISNELSRSRVHQLRGRVDAIVVGIETVRRDDPLLTARPPGPRTA
                     VRIVSDSTGRLPLDSQLVRTACDVPVMVAVSRHAPAEARRQLEAHGCEVFESPGESYA
                     ERLMALLDELGRRRMTNVLFEGGSQVFGTLFDHQLVDEVHAFIAPKFVGGQTARTPVA
                     GIGVERLAHAQILRNVTWEKIGDDFYVCGRIDSA"
     gene            12450..>14366
                     /locus_tag="PLD05_10490"
     CDS             12450..>14366
                     /locus_tag="PLD05_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOP77910.1"
                     /translation="MRTVRVLWPSVFAILAVLYGREVLATDWPLDNNAVLESWGFVNV
                     AARPFEADPSGQRDSTEAIQKAINYARERQLVCLFPPGEYLISDTIVCEQYRPLRTPT
                     RRSGDRNHPCVLMGMERDGRRPCLVLSPQSKGFDDPDHPKPVVYFWAAGNGEEAPLDQ
                     PQPNISMNQMIVGVDVRLGPNHPGAIGISHRAAQGSGIQDCTIDARGAFCGVEGGAGS
                     GGSHAGITVIGGRYGLDLRGTQPAATVTGVTLIDQDESAIICSGRQATTIVGFRIRTK
                     GNGPVILARQSGIHQGQLNLIDGTVEAEGSTSAVLLDTTAAAYFNNVFTRGVGKIVQS
                     PVGELKAVDAKWCHIQEAAIAVPPAGFRGRQLAALGVKELTYTCGIWLDGQKWSGPIW
                     HRLSATEAAPDDLVSRHLWDKQFPRPWSPGVVNAKLLENPPRGDGVTDDTEALQKAIE
                     SHPALFIPKGEYRITRTLRLRPDSQLIGAHRCFTWLVGYGDAGGDFARADQPKPIVQT
                     ANDPNARTILGFFGIRLDISGVGGYALDWAAGGQSVYRDVNVVMPTRWNVPKGAAAPQ
                     FDHPFVLIHGHGAGKWYNFHQESWQLQGPHYRHLLIEGIKGPLHFYQCNAEHSRSDAN
                     MEIRRASGVFVYGLK"
BASE COUNT         3130 a         3963 c         4072 g         3101 t
ORIGIN      
        1 agcctgtctc aaaaagtccc cctctccctc cgggagaggg ttggggtgag ggcaaaggaa
       61 cgttggaatc gattgggaaa actcgcagcg attcgagcca ccctcacccc cgtcccctct
      121 cccgccgaac gggcgagggg agattatggg ataggctcta agtaggcccc tttctgcggc
      181 gtattgaagg gccatggctg cttggccttc gaaactcgtg tggcctccaa atcccagaag
      241 ggcggcgatt ccgtcgaaca atcggcccat actggaggtg cgcggggatt gcacatttcg
      301 ttccatcgcc cgcagcagga ccttcaactc ttgaggggtg aagaacttct ccagcgtctg
      361 ttccagagaa cggtcccctg tttcatagat caggccgaga agtgaccggc gcggctgccg
      421 gaccacaata tcgcctcctg gcagggggaa tgtccgcaat cgggcgacgc ggcaaaatga
      481 tgttccatcg aacaaaaaga actccccacc ccagctcgtc ccgtcggtgc cgtaacctgt
      541 tccgtcccag ataacgccca agatgggcaa ttctccagat ggatgtcccg gtgttgtcgt
      601 ttcttgggac aaggccaact cattgacggc acgacgtgct tcgtcgcttt cggcctcggc
      661 cagacatgcg gcgaaatggg ccacgtggtg ttgccagcga aacacgggga ttttcaggcg
      721 gcgggccaaa tcttccgcgc tgcgggtcga ggcgtaatcc ggatggagat cgcagctaag
      781 aacctcgggt gccgtgtcga aaaagcggag caaatcctcg atcgcgcggc gatgggcctc
      841 aaaggccaag ggattgtcca ggtccccaat atgcgcgctc aggatagcct gatttccaat
      901 ggccagtcca accacgtttt tcaaatgtcc gcccgtagca aggatgggga cttgatggcc
      961 cgacagagaa agcgatcgtg gagcgaaacc ccgagcacgt cggaccatga ccacgtttcc
     1021 cgaccgatcc gtggccataa cggaatcatc caccggtcga acaatgggtc ggttatggac
     1081 aagaaagacg tcggcaatcc cttgtaatcg gtgaagagcc tccgcgttgt caatggccat
     1141 gggttcttcc gagatattcc cgctggtgca aacgatcggt cggttgacgg tttccatgag
     1201 gagatgatgc aaaggcgtgt acggcaacat aacgccccaa tacggattgc ccggggcaac
     1261 gctttctgaa atcgcccacg ggcaggactc atgatcttga gtgttttctg cgtctttccg
     1321 cggcaggagg ataatcgggc ttgccggcga tcgcaaccga tcccacacgg tttggcttgg
     1381 gacgacacaa gagagacgga tcatttcttc cgtcatcatc agggcgaaag gtttgtcggg
     1441 acgccgcttg cgaatccgca gttgcctcac cgcctcgcca cttgttgcat cgcagatcag
     1501 ttggaatccg cctaatccct ttagggcgag aatctttccc gccaacactg ccaacgcagc
     1561 ttgccggagg gcttcatcct tttccgccac aatctgacca ttgcggttcc ataattccaa
     1621 gtgggggcca catcgcggac atgcgatcgg ctgggcatgg aatcgccggt caagagggtc
     1681 acggtactcc cgctcgcaat cgggacatag acgaaagccc ttcatggaag tgagcgggcg
     1741 atcgtacggt acgccttcca caattgacca ccggggacca cagtgggtgc aattgatgaa
     1801 tgggtagcga tatcgccgat cggttggatt ggccaactcg gctaggcatt cccggcaaac
     1861 agccaagtcg ggtgggatgt tgggccgtcg ctcccctccg tcatcgctgg agacgatgcg
     1921 gaatcccgcc gcttctgccc cgcctaaggc gggcaccgag gagcgatggt aattgtcaaa
     1981 ggcggcgcca gccggtaacg atttccaaaa atcctcccaa aatgcccgga ggacgtcggg
     2041 ctgaccttcc acttccagga tcacaccatc ggccgtattt ttaacccaac cttgcaatcc
     2101 ccacttatgg gccattcgat agacaaaggg acgaaacccg atcccctgga cgaccccaga
     2161 aacggtgatc cgccaccgct ggccgtcgcc gggagcttcc acgttctccc aaggcggggc
     2221 tttagggggc aggaaagtgt cgttatggcc gttttctgat ctcttgttca caacgccatc
     2281 aattgggctg ggtcacgcgc acccgaagca aggaccactg gcaagactgc ccataatggg
     2341 gcacactgat ttttacagcc tatcccacaa cctcccctcg cccgttcggc gggagagggg
     2401 ccggggggag ggtgactcga atcgcttccc cgtcccccaa ccctctccca gagggagagg
     2461 aaattttgtg ataggctctt aggcgctaaa tgtcccgcga cggcgcgcgg gggagcactt
     2521 tcttattcta cgccggacgt tgaaaatgtg cccaacggca ggacaaatcg tcccctaacc
     2581 gacccccgct ttatgttcaa gatagaccaa ctcgcttatt tgcgaatcat tgccggcgag
     2641 gatcgctccc tcggggcgtt ggctatccgc agcctccttt tgccggcgga gtttatttac
     2701 gttttggcca tgcgttttcg gaatctggct tatgagttga agtggcttaa ggtgcaatcc
     2761 ctggaggtgc ccgtcatttc tgtgggcaat ttgacgcttg gcggcaccgg gaagacccca
     2821 cttgtggcct ggctggcacg tttctttcgg agacaaaata tccgggtcac gatcatcagt
     2881 cgaggatatg gggcagccga aagctcccga aacgatgaag cacgaacatt ggagcggctc
     2941 ctgcccgatg tcccccatct tcaaaatcct gaccgtttgg cagcggcgga ggttgcgatt
     3001 cgagaattgg caacccaggt gatcattctt gacgacgcct tccaacaccg tcgcatcgcc
     3061 cgcgatttag acattgttct gatcgacgcc ctatcaccgt tcggctttgg acatgtcttt
     3121 ccccgggggt tgttgcggga acccgtggcg ggcattcgcc gggcgaaagt cgtcatccta
     3181 tcacgtgccg accttgttga cgagttgacc cgcagaaggc tccgagacga agtcttgcgg
     3241 attcatcccc aggcacaatg ggctgaggtt tgccatgtcc caacatgctt ggtagcggcc
     3301 aacggcgagg agacagctct ggaaacactt cgcgggcaac gagtggccgc cttttgcggc
     3361 atcggaaatc ccgaaggctt tcggcagacc ctcttgcgat tgaactatga cctggtggat
     3421 tttcgcatct ttccagatca ccacccctac acgcgggaag acctcgaaga attgaccagg
     3481 tggttggaga gaatcaaagc ccaggtggcc atctgcacgg aaaaagacct tgttaagatc
     3541 gggcttgatc aattagggca gattccactc tgggccctgc gagtggagat cgaattcctt
     3601 tcagggcggg aggtccttga ggctcaattg cgtcccattg tggaaaggat tctcgctaca
     3661 cccgacccct tgttctccac gtagcctttt ttccaacgca attagcgttg ttcaaccgat
     3721 ggtgcttccc gtggcagcca ttcaccgatg ttagcattga agacctgatc ttttgcaccg
     3781 tcggccttca ttaccacgga gaagacctgg atgcctagtt cccgttgatc ttgcgaacgc
     3841 gggatgactt tttggggaac ccaagtcggt gtgcggattt caatcatgac acgatcggaa
     3901 gattggggcg gaagggtgac ggtggttgtt ccgcgctgaa tgtttcccaa cgtctgacca
     3961 ctagagacga gagtgatttc tgcgtcgtcg agcactttag ggatttcgac tgaaatggtc
     4021 aactgatagc cccggcctgg gatgactggc aggaggagcc gggacgaacc gctggtccag
     4081 cgaaaaggcc gaaaaccctt ttccgcaccg tgccagccga gaagatagcg ggcatcgccg
     4141 gggcggccaa tttcaagttg gtagtgttct tttgcttctt ctgtccattc gaccgggtcc
     4201 acggcgttca ggccaatttg agcccggttt cccgcttgat tgtttacccg tagccctccc
     4261 atcgcttggt ttcccaccac caaagccgat cgcacatctt tggaaatcgc tagatggacg
     4321 gatgaatcct gatcgaagga acaaccttgg atggtcaccc aacccgcgtc aacctgaata
     4381 gccggtgaac cttggtaagc cacatcccac tggcaaaaat ggcaagccat gagggtcgtt
     4441 tggacatctt cgcttcgcag ccaaacacag cggtcgattg gcccccaaaa tgaacaattg
     4501 accaggctga ccctgccggt ggcttcggga actacttcca gggttacggc atccttgctg
     4561 ccccaccgcc ctacaaattc accgttggtg attaggagtc ccggcggttg ggtgctttca
     4621 acaagcacgg cccgctggca gcagtcggcc ccaatcccaa gaaagttgcc attggcactg
     4681 cccttcgacg acttcgaaaa cttgtatccg accccatagc caaaacagaa ggtgttaaac
     4741 acgtagtgcc agtccgtccg ggcaaattca aaagctacac cgttgaggtt aatccactcg
     4801 caatagggat cgttgggacg ataggaaacg ccaaagggcc agaagtggat gttctccacc
     4861 cgcccaatgt cgtagcactc atcgacatag agtccccgtt tgatgggata acccgtcacg
     4921 ttgcgaacca aatgacgatg ggcacgaacc aagcgaatcg cctcgtaggg gttcacgagg
     4981 agacagtctt gaatggagac attctcggtg ttgtaggagg ccacacacgg tggatatggt
     5041 acgggcggca catcagattg cttccattcc gggtaaacca cggtcagccc tcggatgctg
     5101 gcatgccgac cagccaaacg aatgaatggt tcgccttcct ctgagccgcg accttcgtaa
     5161 gcaaggagca ccgatccccc cacctcctgc catttggccc atcgggttgt cggggccgtt
     5221 tgcaaaaccc cctggagcgt cacatcttgc ggtaccacca aatggccata aattcggtac
     5281 gtccctgccg ggatctccac aataccgccg cctcggcgcg acatttcgtc catcgcccgc
     5341 tggatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgca ccattcggta
     5461 ggccagacgt tggcccgacc tgaaaacacc gtttatcgca tattacgtct tttgcctatg
     5521 atagcgtatt tactgcggtt tatcctaatg ggaggatgta gcgggctatt ttcgagggct
     5581 ggaaggccca tggagaacgc acacgtcatt gtctggattt agcgcctgca acttgttaag
     5641 ttaaagatag cgcggttctt gttgggggtc atcttttgat cacgttattg cgggaaggct
     5701 aaatctgtgt aacgtgcggg atcggcgatc atgacggtcg agcagttcgt tgacctattg
     5761 aaacgaagcc aactgatcga agctgatcag ttgcaaaagc tgctcgccga atatctgccg
     5821 cccgagggac catcgtcgct gactgccgaa gagttggccg aaaaactcat cgcggcgggc
     5881 atcttaaccc gctggcagtg tgacaagctt ttggagggcc ggtacaaggg gttcttcctt
     5941 ggtaaataca aactgctcga tcaccttggg acgggtggga tgagtagcgt ctatctggcc
     6001 gagcatgttc tcatgcagcg ccgggtggcc attaaggtcc tgcccaaaca ccgtgttcag
     6061 gatgcctcct acctggctcg attcctgcga gaggcaaaag cggctgcggc gttggatcac
     6121 cggaatatcg tgcgggccta tgacgtcgat aacgatggtg tgaaccacta tcttgtgatg
     6181 gaatacgttg aggggcagga ccttcagcgg atggttcgtg agcgtggacc acttccttac
     6241 aatgaggcgg cggagtacat ccggcaggcc gccgaaggcc ttcaccatgc ccatcaggcc
     6301 gggttgatcc atcgggatat taaacctgcc aatcttctcg tcaataaaaa tgggacggtc
     6361 aagctcctcg accttggtct ggcccgattt accgatgacg agcgaggttc tctgacaatt
     6421 gcctatgacg aaaatgttct gggaactgcc gactatctgg ctccggaaca ggcagtcgat
     6481 agtcaccgcg tggatcatcg ggccgacatt tatagtctgg gatgtacact ttactacttg
     6541 ctgacggggc acccgccctt ttgtgaaggg acgcttgccc agcggatcat gaaacaccag
     6601 cgggaggccc cgccaagcat tcgtgatgaa cgtccggatg ccccagaaga cctcgtggcg
     6661 atttgtctca aaatgatggc caagaagccc tcggcccgct atcaaacggc tgcggaggtg
     6721 gcccaggccc ttcggctatg gctgatcaat catgggtatg aggtcccgga aagcgacacc
     6781 cggctgccga aacctgtcaa ggctgccccc gcgggcacaa ccaaggagaa ggcacccgtc
     6841 cgaacacttg tctcggccaa ggctttgccc gattccgcgg cggtaccaga cacaaagaca
     6901 gccctacaaa cggcgccacg cgagacttcg cctaagaccg aacaatcgtc gcctaatctc
     6961 ggtgtaccgg tggctaagcc gttggaaggg gcaaacacat tgccgatcgc caaacccctt
     7021 gtcgaaacgg cgaccatccc ccatgaactt gtcgatcaag cgggggatga ggcggaagcg
     7081 tctccctttc ccttaccact gcctgcggat gaagacccac gattggcctt ggtgcttggc
     7141 ggacgggccc gacgcagcgc acccccgatg cccttttgga tttggattat cattgccttg
     7201 gggcttgtga cggccgtcgt tttggccatc attcagttcg tgtgatggcg aatcctccgc
     7261 tggttcatga ttcgcctttt gccgtggaga atgaagttct ataattcggc aaagcaggcg
     7321 gttttcaaga ttccctagaa ctttcagcga tgttttgaga ctgggcttaa cttctacttg
     7381 gacaagtcag tatgaaagaa agacataaga ttctcatcgc agatgataac ccgacgaatg
     7441 tcgagctatt ggaggcctat ctggccgact acgattgcga gattgccgtg gccagcgacg
     7501 ggctggagac ccttgccatg gcccgaaaga ttcaccccga cctcatcctt ctcgatatca
     7561 tgatgcccaa gatgagcggg tttgaggtgt gccagaagct gaagagcgat cccgccacaa
     7621 aagatatcat gatccttatg gtgacagcac tcaatgagtt gggggacatc gaacgggcgg
     7681 ttgcggcggg ctgcgatgat ttcttgagca agccgattca caagctggaa ctactcaaac
     7741 gcgttgagaa tatgctaaaa ctgcgacaac tgaccgatga attggcacgg cttcgggcct
     7801 acatcgacgc catggaagaa acccgcccag gccaacgcgg tccagccaaa gggtactaac
     7861 acttgcccta agggtggctg acgggattcg aacccgcgac acccagatcc acagtctggt
     7921 gctctaacca gctgagctac agccaccata tcagggatat ttgccgcccc gcgatgaccg
     7981 tgacggataa ggctgtggtc cgtcgcggaa gtcaacgacc aggagctagc cgtcaatcac
     8041 gggaccagtc atcaatactt ttagtctatc cggcctctcc tgcgcggtca acgatgggtg
     8101 gcacttgacc ggcgtgcttg cgtaactaac gtttgtaaaa ggatttcagg tcatttgctg
     8161 cccaagggct tgggctacac tcatgcctta gaccaatggt attccaaaac aacccagacc
     8221 ccggggaggg gaacccgggg tctggcgacg acgtcgcagg gacgtcctcg gaggggtttt
     8281 tcggaaattg tgtcgggcta attgcgattc ccaaattcaa tcaggtcatg taaggtactc
     8341 aggcatcggc tcacacctga tctttttcac ggcgacgcca ccttcggagc acggatacgc
     8401 cgcctatggc gaccgtcgaa aggccaagaa gaactacgct cgtcggctct gggatgactt
     8461 caccgttact gcccggcgtc tggaagacta aattatccaa ggcaaaacac gccggggtat
     8521 tcatacccca agggcccaca tctgaggaat cgagctcaaa ttccaaaatg cgggcagtgc
     8581 tgataggtgt caaatcgacc gtttgccagg tattcacgat gtagtcttgc gcgttatctt
     8641 caaagcggaa atcagcgagg tagaactgaa ccgtcccgag taagctattg gcaaggtcct
     8701 ttccgtaaat gatcagtttg aaccagtcgg gatcgttgcc tgaggggcca ccgaatttct
     8761 tggcaaattg atcgccttcg agcatcgagt agtaggcata ggttgtattt gtcacctggg
     8821 cggaaacgag ggacacacct tccgggactt cgattctggg caagtcaccc cacaaattga
     8881 cgaagctcac ggcgtaggtc gcagagttgt cggcccccga tcccgtaatg gccgaatact
     8941 ggttcagata cccacgagtt gttgtgtcgg tcttgttgga ataggcaaat ccatcccaca
     9001 cgtcccacgc cagggtgtaa ttattgtgaa agatgaatcc attcgaagtg aacgtattgt
     9061 gaccgtcatt gggggcgccg ttccagtaac tgttggccgg aagggaggcc ccaacatcct
     9121 caaaatcgac cgtaccggcc tggatcgagt tcaagggaac cagtagtccc aaaacgaaca
     9181 ggctaggcag ccagaggcac gatctggcca agggagtttg tgcaagcgaa ctcatgatgc
     9241 tcctcctttg ttgaaacgta aaccaacaaa acacgccaac atagtgttcc tgcttcgata
     9301 aaccggctta cagcccggtg accacttcgc cacccgcacg tgtgcagatg gcggccaaaa
     9361 cgctcaaatc catcgtctct gccaagaaac ggaccgaggc atccgtaaac aggccgttgg
     9421 caccgcctgg atgcttgctg cggatatcgt tttcaaacgc gggtgcctta ttaatggcat
     9481 aggcctgatc aaaaacgttt aggccgttga tccattgacc atcgcgaaag tcggagtcct
     9541 cggagacaat gagcgtattt gatgttccat cgatgacatc tgccacagta agcgcctgat
     9601 catagagcat catccctttg gggggattgt tgggactggt gatgcgctcg ccatagatac
     9661 ctccgtaatc gcatggtccg cgaccctgcc gcagctctcg ttgcgttgtg gcacttgggc
     9721 aaatatagag gggcaagatc gtggcggcgg cctcggcatt ctcgggtgcg tcgaacggtt
     9781 tactcgtatc aactttctgg tagagtggct gttgttcaac atacgggagg agaaatagcg
     9841 accaggctag ttgccgccct gccggtccat agggtttttt ggtttgtgga ttgatcatgg
     9901 cacgatgttc aatcccgccc ggcggaaaac atcctaaagc ctgatggtag ttatgcaggc
     9961 cgatgcccat ctgccgcaaa ttatttgtgc aggccgagcg gcgggcggct tcacgggcgg
    10021 cttgaacggc cggcaaaaga agcgctgtca gcagcccaat gatggcaatt accaccagca
    10081 actcgacaag tgtgaagcct ttccgtatca cgcgagaaaa tagccgtaac gtcctctcgc
    10141 cgcacctatt ccgaagcagg gcagagcgac ggtccatcgc aaacctcctg ccgctatcgt
    10201 gcagaaagca accctagcgc tactgggaac atactgtccc cacgcgtggc cgaaacttaa
    10261 tgtggctgat gagttgcaac ctgctcgata gcctcaagta aggtccagaa ctccgcagcc
    10321 tccgtcccac gcagaggact gacagccatg cgttggtagg tcttctgact tccgagctgg
    10381 actcacccgg ccttctcgtc gcaacgattc ctcatgggat tagaccatta ggaacctgct
    10441 ggctgccgcg acaatggcca aggagactgg gcaagtctcg tggcgctcgg ttacagcggc
    10501 ggggaccgtt ccggattctc accggattcc ctgttctccg atcaggctag ctgcccgacc
    10561 ggacaccaac gcccaacacg gaccatccta gcgttgctac ctgcccgtgt caactgccca
    10621 agattccaaa atcgaaacgc ccagcttcct tggattcaaa atgatgacaa gattagtaca
    10681 attaacggag ctgggagaaa gagcatcgca gggatggcct tttgtgggca tttgttttaa
    10741 cccgtattgc attcgtccca gggcacctca aacaaggggg gagattatgg atcaggccga
    10801 tctcgatgcg tggcacatgc ggcgcgtctt agaactggcg aaacgcgggc agggatccgt
    10861 tgagccgaac cccatggtgg gttgcgtgat tgcaaagggg gcggagatca tcggagaagg
    10921 ttggcatcgt gcctttgggc agccgcacgc tgagattgaa gcgctccgtt tggccgggtc
    10981 tcgggcagcc ggggcaacga tgtatgtcag ccttgagccg tgctgtcact acgggaagac
    11041 acccccttgt acacaagctg tgatcaaggc agggattcgc cgcgtggtgg tggctatgaa
    11101 ggaccccttc cctcttgtcg cgggaaaagg actttcggaa ctcaacaagg ccggcattga
    11161 ggtcagcgtg ggtctgatgg aggcagaggc ccgatctctg aacgcccctt atattaagct
    11221 catcacgcaa cagcgcccat gggtaatcgc caaatgggcg atgacccttg acggaaagat
    11281 cgccacctat agcggtgaca gtcgttggat tagtaatgaa ctttcgcgct cccgcgtcca
    11341 tcaactgcgc ggtcgtgtgg atgcgatcgt cgtcggcatc gaaacggtgc ggcgagacga
    11401 tccgctgcta acggcccgtc ccccgggacc gcgcaccgct gtgcggattg tctcggattc
    11461 gacagggcga ttgccgttgg actctcagtt agtgcggacg gcctgtgacg tccccgttat
    11521 ggttgccgtt tcccgacatg cccccgctga ggctcgtcgc caattggagg cccacggttg
    11581 cgaagtcttc gagtctcccg gggaatcgta cgctgaacgg ctcatggcac ttctcgacga
    11641 actgggtcgc cgacgcatga cgaatgtcct cttcgaaggt ggatcgcagg tctttgggac
    11701 tctctttgat catcaactcg tcgatgaggt ccatgctttt attgccccta agttcgtcgg
    11761 ggggcagacc gctcgaacgc ccgtggcagg aattggcgtg gaacgacttg cccatgccca
    11821 gattctgcgg aacgtcacct gggaaaagat tggtgacgac ttttatgtgt gtggccgaat
    11881 tgattcggcg tgattcatag ccacggattg ccctggcacc cttgataatg agaacccctc
    11941 ctaacgtggg gtgtgtggac atctatgtgt tttccaaaca cttcgtagac gcaggcccac
    12001 gcgcttgctg acgcccgcac catagcacat aaccacaaca aaccggcaca accaagggag
    12061 ttgcctctat taagagtcga tcccaaaaag tcccccctct ccctccggga gagggttggg
    12121 gtgagggcaa gcgaacgtcg gaatcgattg agaaatccgg cagcgattcg agccaccctc
    12181 acccccgacc cctctcgcgc cgaacacgtg aagggagatt atgggatagt ctctaaatcg
    12241 gcacacgcgt tccggtcgcc ggtaagggcg aaccttgtgc ctacttggcg tgccgatcaa
    12301 cgccgttttc cgcaatggcg ggcccccggg ccatcgttgc acaaagtggc cggtgattgt
    12361 cctgaagcgg gtaatggttc acaatggggc agacgttcaa tctgagagac attggttgcg
    12421 agtctcaatt caatgctgcg ggaggtatta tgcgtactgt ccgtgtgctg tggccaagtg
    12481 tttttgccat tcttgctgtg ttgtatggca gagaagttct cgcgacggat tggccgcttg
    12541 ataacaatgc cgtcctcgaa tcgtggggat ttgtgaatgt tgccgcgagg cctttcgaag
    12601 ccgatcctag cggtcagcgc gattccactg aagcgattca aaaggccatc aattatgcga
    12661 gggagagaca gcttgtgtgc ctctttccgc ctggtgaata tctgatttcg gacaccattg
    12721 tctgtgagca ataccggccg ttgcggactc ctacgaggcg ttcgggagat cgcaaccacc
    12781 cgtgtgtttt gatggggatg gaacgggacg gccgtcgccc atgtttggtc ctttccccac
    12841 aaagcaaggg atttgatgat cctgatcacc ctaagccggt ggtctatttt tgggccgcgg
    12901 gtaacggtga agaggccccg cttgaccaac cccagccaaa tatctcgatg aatcagatga
    12961 tcgtgggtgt ggacgtgcgg ctggggccga atcaccctgg ggcaatcggc atttcgcatc
    13021 gggcggccca ggggtcaggc atccaggatt gcacgatcga tgcccgcggg gccttttgtg
    13081 gtgtggaagg cggtgccggt tcgggcggca gtcatgccgg cataacagtc atcggggggc
    13141 gatatggctt ggaccttcgg ggaacgcaac ccgccgcaac ggtcacgggg gtgaccctga
    13201 ttgaccagga tgaatctgcc attatttgtt ccggccgaca ggccacaaca attgtgggtt
    13261 ttcggatcag gacaaaaggc aacggcccgg taatcctggc caggcaaagc ggaattcatc
    13321 agggccaact caatttgatc gacggaactg ttgaggccga ggggtccacg tccgcggtgc
    13381 tgctcgacac aacggccgcc gcgtacttca acaatgtatt cactcgaggt gtgggaaaaa
    13441 tcgttcaatc cccggtggga gaactcaagg ctgttgacgc caaatggtgc cacattcaag
    13501 aggccgcgat tgccgttccc cctgccggtt ttcgcggtcg tcagttggcg gcacttggcg
    13561 tgaaggaact cacttacaca tgtgggattt ggttggatgg ccagaagtgg tccgggccta
    13621 tttggcacag gctttcggcc acggaggcag ctccagacga tttggtgagc cgtcaccttt
    13681 gggataagca atttccacgc ccatggtcgc cgggcgtcgt caatgcgaaa ctgcttgaga
    13741 atcctcctcg gggtgacgga gtgaccgacg ataccgaggc ccttcaaaag gccattgaga
    13801 gccatcctgc cctctttatt cctaagggcg agtaccgcat taccagaact ttgcgtttgc
    13861 ggcctgacag ccaattgatc ggcgcgcatc gttgctttac atggctcgtg ggatacggag
    13921 acgccggggg agattttgcc cgcgcggatc agccaaagcc gattgtccaa accgctaacg
    13981 accccaacgc gaggacgatc ctcggtttct ttgggatccg gctggacatc tcgggcgtgg
    14041 ggggatatgc ccttgactgg gcggccggag gccaatcagt ttatcgcgat gtcaacgtgg
    14101 tgatgccgac tcgctggaac gtcccgaagg gagcagcggc accacagttt gatcacccgt
    14161 ttgttctgat tcacggacat ggggccggca aatggtacaa cttccatcag gagtcttggc
    14221 agcttcaagg gcctcattat cggcacctgt tgattgaggg gataaagggg ccattgcact
    14281 tttaccagtg caacgcagaa cattcccgaa gtgatgccaa tatggaaatc cgcagggcct
    14341 ccggtgtttt tgtgtatggt cttaag
//
LOCUS       DAOSUT010000107        13694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2684_length_13694_cov_9.467336, whole genome shotgun sequence.
ACCESSION   DAOSUT010000107 DAOSUT010000000
VERSION     DAOSUT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_13694_cov_9.467336"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLD05_10495"
     CDS             <1..297
                     /locus_tag="PLD05_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77911.1"
                     /translation="YAIWVTIGYFEVQPVAEVMQPDPNVPDNFLINGMSFSPAEIAEI
                     YPDGYMLGQELGSDTGEIKRHRAFFLFDRTIPVGFRRGYDLNVDQGLLIKRIIE"
     gene            337..1125
                     /locus_tag="PLD05_10500"
     CDS             337..1125
                     /locus_tag="PLD05_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOP77912.1"
                     /translation="MSGEDVVNPEGDGLAEDKAVSLEDLARAFAEATGQSFDLPQGDE
                     TTEIDSIGEPSEEAAVPDAVAASAEADVPATPKSILEAILFVGHPHNQPLSPAQAAEV
                     MRGVQPEEIAHLVTELNAGYEATNRPYRIVFEGGGYRMKLLPEFQTLREGFYGKVRQV
                     RLSQQALEVLAIVAYKQPITVKEVNQMRGRASGALLAQLVRRDLITVSTPVDLTGKKG
                     VPQYRTTQRFLDVFGLQSLEDLPQLGEASLIKPLDDSTPSTPGS"
     assembly_gap    1287..1386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1387..1937)
                     /locus_tag="PLD05_10505"
     CDS             complement(<1387..1937)
                     /locus_tag="PLD05_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77913.1"
                     /translation="MDASPQTAASEFRDPTRVLGRWLGELSAGQQELAGVLDAMFAEL
                     TTALEDWAKVQAAHVAHLQQREKELAERAASLEQSLAEQRRLQDELAQLREQLNARAA
                     EMDRQAQAIQAQVEELQRRATEAQHSEAEWQSLREIVVQLRSLTATRPPSEAAGGAER
                     SHPDVSQWEERIRQLEEDLNEAQR"
     gene            complement(2444..3487)
                     /locus_tag="PLD05_10510"
     CDS             complement(2444..3487)
                     /locus_tag="PLD05_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77914.1"
                     /translation="MGEKVRFGVIGAGGIAFRKTIPAMLRARNLRVVAVMDTANVEKV
                     AEAFRIRRAYTTAEDLLADPEVDAVYIASPVHCHLEQIELAAAARKHILCEKPLTRDV
                     AQTRRAIDVCRRKKVFLQEGYMMKFHGAHQVIRENILQGRLGKVVYLRAQLSCWYPPI
                     PGAWRQDPKTGGGGALIDMATHLLNLLEFFVGPIRRLAALTGNLVHPYRSEDASTILA
                     EFRSGAHATIDCFWCIPDEASRTRLEIYGSAGAIFTEGTIGQSVGGTMEGIFGLGSAE
                     YDAAQNKDVVRKFRPIPFKKINPYQAECEYFAQCILDGRPPEINGPVEALRMARLVEA
                     AYRSAKEKVFVPV"
     gene            complement(3562..4851)
                     /locus_tag="PLD05_10515"
     CDS             complement(3562..4851)
                     /locus_tag="PLD05_10515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP77915.1"
                     /translation="MSFDYIVSQKLAIDGGPRAVTNKLPGWPQFSEEAIRAVEAVLRS
                     GRVNYWTGPHGMAFEKRFAEWQGSRFAIAVSSGTAALHVALSALGIGPGDEVIVPSYT
                     FIATSFAVLQAGAIPRFADVNLEDHCISVESAEKLVNERTRAIIPVHLYGNVCDMDKI
                     NDFAKRHNLFVIEDNAEAFGGEYKGKKTGTLSHIAACSFCQNKTFTTGGEGGMVTTDD
                     EDLAWEARSFRDHGYDVKERLNLLELEQKLPYIHTRLGWNYRMTEMQAVIGLAELDRM
                     DTWNMPRRRRNAHILMDALMPLPQIKFGPIDTEERRNGFYVCAFSLDIENMGCDIQQF
                     VKAATAEGAPVWKVFWPQCHTERAYRERRGFGRTGFPFTSKEYTNPESVDYSNVEVPN
                     ALWHETHTFTCFAFPTCSEEDMQQIAAALVKVIKAYS"
     gene            complement(4973..5206)
                     /locus_tag="PLD05_10520"
     CDS             complement(4973..5206)
                     /locus_tag="PLD05_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77916.1"
                     /translation="MLGTAATALGQTKGVKGQLLKGRAETGSGFIDPRAGYGGRTWGI
                     RERPHGGELTLAPIAQVVRFSGPLIASLPEHLQ"
     gene            5717..6355
                     /locus_tag="PLD05_10525"
     CDS             5717..6355
                     /locus_tag="PLD05_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOP77917.1"
                     /translation="MTYQESDQAFEPEAGAQAPSPGQEAPRSAQAQPDAISGQYERPP
                     RRGDPDIELWKGGYSMRAMVATWILLGLITIVVLVLDVWITQNFLKEHQALLWEITLG
                     FLVLVWGYFILVGLYKAATIRYRLTTYRFYHQKGLIFRTIDSMEVIDIEDLALHQNLL
                     ERLVNVGRILLRTKDPSDPLLMLYGVYDPHEAFKRIDKARRDEHVRRSIKIG"
     gene            6451..7323
                     /locus_tag="PLD05_10530"
     CDS             6451..7323
                     /locus_tag="PLD05_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP77918.1"
                     /translation="MKRRTFLLTLVVISLSAVPVLAAPYKALIVTGQNAHDWKGTTPV
                     LKQLLEETGLFTVDVAQSPAKGEDMSSFKPEFKNYHVVVLNYQGDSWPEETKKAFVDY
                     VKNGGGVVVYHFACAAFPDWPEYNEIIGLGGWGNRNEKWGPYVRWRNGRVEFDFSPGR
                     GGGHGPMQPFAIDVRYPEHPIMKGLPPRFMHVPDELYGWLRGPAKNLTVLATAFAPKD
                     KGGSDEHEPILFTVEYGKGRVFFNALGHTANELKSVAFIVTYQRGAEWAASGKVTQPV
                     PDDFPTADEARVRP"
     gene            7520..9160
                     /locus_tag="PLD05_10535"
     CDS             7520..9160
                     /locus_tag="PLD05_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOP77919.1"
                     /translation="MTRRRQDFGQDQNEIARLDDLLRRDHTLIGGVLFGLQGHLVEVQ
                     ARALEVLRRSIPWREAVNISGMANLAVRESLNRISGAFAKYAIPEPQVAIQINLAPAG
                     IWKEGTWLDLPLAIIMLQASGYLPDLPETIEGEYVLVGEVGLHGEIRRVPGVLSIAEA
                     AMPGQTLVVPSGNERECALITARSDRKSCRVAAVSTLEEVIEFFQGKRKLENVLKEEI
                     RFEKVGEKPLDFGRIKGQERAKEAAVIAAAGGHNLLLIGPPGEGKSTLARAMAGIMPK
                     LTIDEKVELTRIYSAAGLLKTDGVVVSYRPFRAVHHTASKQALVGGGTDVIRPGEVTL
                     AHLGILFLDELPEFRRDSLEALREPMETGEITISRAKARGTYPARFTLVAAMNPCPCG
                     YYPSPACTCSREKVKAYRSRLSGPLLDRIDMQVEMEPLSLEEKLAKTQEGETPRLQRL
                     VQAARDRQTRRFAGTSIPYNAAIPAGRIQDFCQFSSNGFERFKQIVEENRMSTRRTDR
                     LAKVALTVADLEGADNIEAHHVDKAASFVIGDRDIHRD"
     assembly_gap    9302..9401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9402..12145)
                     /locus_tag="PLD05_10540"
     CDS             complement(<9402..12145)
                     /locus_tag="PLD05_10540"
                     /inference="COORDINATES: protein motif:HMM:NF000282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOP77920.1"
                     /translation="MISRFFLDRPVFAWVIAIMIMVLGGLAIYNLPISQYPDIAPPTI
                     RITAFYPGGSAETVENSVTQIIEQKLTGLDDMIYFSATSDSAGSCNIELTFAPGTDPD
                     LAWAKVQNKLQLAMASLPEMVQRMGITVRKATRNYLLIANLISEDGRLGNIDLRDYAQ
                     SNLEKVLARVPGVGEVEVFGSQYAMRIWINPEKLTAYQLTVEDVIRAVRAYNVEVSAG
                     QFGGAPAVPGQRLNVSIVVQNHLQTPDEFANIPIVVQPDGGIVRIRDVARVELGTDSY
                     ASNIQFNGMPSAGLAVRLEPGANALDTADRVKKRLAELSHYFPPGVRVVYSYDTTPFI
                     RVAIFEVVKTLFEAVGLVFLAMLLFLGNLRATLIPTIAVPVVLLGTFAVLGAAGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMREEGLPPREATIKSMGQITSALIGIGLVL
                     SAVFGPMAFFPGSTGIIYRQFSVTIISAMLLSVVVALILTPVLCASLLKPIAKGHEPG
                     ESAVWFLRWFFVGFDRIFFWIRDQYVRVVHHSIRKKVPYLVGYVLILVLIVDLFLRLP
                     TGFIPQEDQGVLMAQITLPTGSTLEQTQAVADKVQQYFLEKESEGVVSCMTIVGSGFS
                     GRAQNNSMVYVMLKDWHLRERKDLRAKAIADRATAALSSIREASIYVFPPPPIRELGN
                     ATGFDFELIDMGGIGHDALMAARNQLLEMAAQHPVLQRVRPNGMEDVAQYRVDIDWER
                     AGILGVPITSIHNTISAAFGGSYVNDFIQGGRVKRVYVQADAPYRMLPEDLQRLYVRN
                     QEGKMVPFDSFATTRWTSGSPRLERFNGFPAVNIWGEPAPGHSSGEAMRAMEELVGKL
                     PPGVGFDWRGISYQERMATTQAPILYAFSILVIFLCVAALYESWTIPFVNLLMLPLGV
                     "
     gene            complement(12148..13464)
                     /locus_tag="PLD05_10545"
     CDS             complement(12148..13464)
                     /locus_tag="PLD05_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOP77921.1"
                     /translation="MNILPRFVAIIGIVLDILLITGCETAPSAAPPPGPPEVAVYTVE
                     TTKVRITTELPGRTASYLVSEIRPQVNGVILKRFFEEGSEVKAGDVLYQIDPAPYQAA
                     YDVALANLKTAQQAVEKARAALEASMAAQKEQEALFHLARTNRERYERMYEQLATTAM
                     ERDQAVTNAEVAEAALLAAKARVETDRKALAAAEAAVQQAEAALEAAKINLDYTQVKA
                     PISGRIGRSRVTEGAIVTAYQSTPLATIQQFDPIYVDIPQSTSQLYRLRRALQQGRLK
                     AEGTDKVKIILEDGTIYPHDATLKFRDVSVDPGTESVIVRVIVPNPEGELLPGMFVRA
                     SIELGEQQDAILVPQQAVQRDSRGEPFVWVVDDQNKVHVRSIVTDRAFGHRWLVLEGL
                     KAGERIVMEGMQRLKPDMVVNVVPWVEPSAAASQESSRGSESANGR"
BASE COUNT         2950 a         3998 c         3719 g         2827 t
ORIGIN      
        1 tacgccattt gggtgacgat cggctacttc gaggtccagc cagtggccga agtgatgcag
       61 cctgacccca acgttcctga taatttcctg atcaacggga tgagcttctc tccggccgag
      121 attgccgaga tttatcccga tggttacatg ctcggtcaag agctgggcag cgacacaggc
      181 gaaatcaaac ggcaccgcgc tttcttcctc tttgaccgca ccatcccggt gggcttccgt
      241 cgtgggtacg atctcaacgt ggatcagggc ctcttgatca agcgaatcat tgaatgaagg
      301 atcgtcacaa cacgctgggc aaggaggttg acaggcatgt ctggtgagga tgttgtgaat
      361 cctgagggtg acggtctcgc cgaggataag gcagtctctt tggaggacct agcccgggcc
      421 tttgccgagg ccacgggcca gtcgttcgac cttccccagg gcgacgagac gactgagata
      481 gattctattg gcgagccctc agaggaagcg gctgtgccag atgcggtcgc ggcttccgcg
      541 gaggcggacg ttccggcaac gcccaagagt atcctggagg cgatcctctt tgttggtcat
      601 ccgcacaatc aaccgctttc tccggctcaa gcggcggaag tcatgagggg tgtgcagccg
      661 gaagagatcg cccacttggt gacggaactc aacgccgggt acgaggccac taatcgtcct
      721 tatcgaatcg tttttgaagg cggcggttat cgtatgaaat tgcttccgga attccagaca
      781 cttcgggagg gcttttatgg aaaggtccgg caagtcaggc tttcccagca ggctcttgag
      841 gtactggcca ttgtcgctta taagcagccc atcacggtaa aggaagtaaa ccagatgcgc
      901 ggccgtgcca gtggtgccct cctcgcgcaa ctggtgaggc gggacctcat tacggtttcc
      961 acacctgttg atctcacagg gaaaaagggg gttcctcagt atcgcaccac gcagcggttc
     1021 ctcgacgtgt ttggcctaca aagcctggag gatctacccc aattgggcga agcatcattg
     1081 atcaagccat tggatgacag tacgccatca acgcctggat cgtagccgat caagcccaga
     1141 cgtcatcgag cagaatttcc cgctcggatc aggacaataa gaagccgtta ctttccgcgg
     1201 cggcggtcaa agtctcgtct gagttgttca aattgagcgg acaaagaatc gagaaaggcg
     1261 tcgcccgtac ccgtccgccg agcttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnncgct gggcctcgtt caggtcctcc tcgagctggc ggatcctttc ctcccattgg
     1441 ctcacatcgg gatgagagcg ttccgcgcca ccggcggctt cggaaggcgg acgagtcgcc
     1501 gtcagcgagc ggagttgcac gacaatctcg cggagagact gccattcagc ctcggagtgt
     1561 tgggcttcgg tagcacggcg ctgaagctct tccacctgcg cctggatggc ctgggcctgt
     1621 cgatccattt cagcagcccg ggcattaagc tgttcgcgga gttgggccag ttcgtcctga
     1681 agtcgccgtt gctcggccag cgactgctcg agggaggcgg ctcgctcggc cagttctttc
     1741 tcccgttgct ggagatgggc cacgtgcgcc gcctgcactt ttgcccaatc ttcaagggcc
     1801 gtcgtcaatt ccgcgaacat cgcatccagg acgccggcca gttcctgttg accggcggac
     1861 aactcgccga gccagcgtcc caacactctt gtgggatcgc ggaattcgct cgctgccgtt
     1921 tgaggggatg cgtccatggc ctaggaaaac ggttaaatgg ctggctttac ggccctcctc
     1981 gttcaatcgc agtctcaatc tcccggcgac gctcccaaca cgtggtcatg cttcggtatg
     2041 cgcatgtttc gtgtcagtgg cactcaatct attgtttcgc tcccatcgtc ggcccacgtc
     2101 ggttaattcg cgacacaaac gaacagtcga attcaagacg cgaccgccac tccctcgacg
     2161 agcaccctca gacaagccgt cttgccaggg tactccatat gttgaccccg ctagaaaaac
     2221 tctagcttac gggatggaca atggcaaatg gcaacgtcgc acccccgctt agagcctctc
     2281 gcacagttcc ctctccctct gggagagggt tagagtgagg gcaagtgatt gtcggagtgg
     2341 atcgagaaaa ccggcaggga ttcgagtcag cctcaccccc ggtcccgctc ccgccgaacg
     2401 ggcgagaggg gaagtgtggg atacgctcta aagggaagcc cccttacacg gggacaaaga
     2461 ccttctcctt ggccgatcgg taagcggctt ccaccagcct ggccattcgc agggcctcca
     2521 ccggaccatt gatctcgggt gggcgaccat ccaagatgca ctgggcaaag tattcgcatt
     2581 cggcctgata cgggttgatt tttttaaacg ggatgggacg gaatttgcgg acgacatcct
     2641 tattttgggc agcatcgtac tctgccgaac cgaggccgaa gatgccctcc atcgttcccc
     2701 cgacactttg accaatcgta ccttctgtaa agatggcccc ggccgagccg taaatctcca
     2761 accgtgttcg gctggcctcg tcgggaatac accaaaaaca atcaatggta gcgtgcgccc
     2821 cggagcgaaa ctccgccaaa atggttgagg cgtcctcgga gcggtagggg tgtactaagt
     2881 ttccggtgag ggccgccaac cgacgaatcg gtccgacaaa gaactccagc aaattaagca
     2941 gatgcgtcgc catatcaatc aaagccccac ccccgccggt cttagggtcc tgtcgccatg
     3001 ccccggggat cggcggatac caacaggaca gttgtgcccg caggtaaacg accttcccca
     3061 accgcccctg caaaatattt tcccgaatga cctgatgggc cccgtggaac ttcatcatat
     3121 agccttcttg caggaagacc ttttttcgtc ggcaaacatc aatggccctt cgtgtctgcg
     3181 cgacatcccg ggtgagcggc ttctcacaca agatgtgctt acgcgcggct gctgccagct
     3241 caatctgctc caggtggcag tgtacgggag aggcaatgta cacagcatcc acctcgggat
     3301 cagccaacaa atcctcggct gtagtgtagg ctcgacgaat gcgaaacgcc tcggccacct
     3361 tctcgacgtt cgccgtatcc atcacggcca ccacccgcag attccgcgcg cggagcatgg
     3421 caggaatcgt cttgcgaaaa gcaatcccac cggcgccgat gaccccaaaa cggaccttct
     3481 cgcccatgat acggctcctc ttttctaaaa cgatgttttg cctcaattcg cccagaaaag
     3541 cccgtcctag cccttggatc gctagctgta cgccttgatc accttcacca aggccgccgc
     3601 aatctgttgc atatcttcct cggaacacgt gggaaaggca aaacaggtga aggtatgggt
     3661 ttcgtgccac aaggcgttgg ggacctcgac gttggagtaa tcgacgctct cggggttggt
     3721 atattccttt gatgtaaacg gaaaaccggt tcgcccgaag ccccgccgct cacggtatgc
     3781 ccgttctgtg tgacactggg gccaaaagac cttccacacg ggggcacctt ccgccgttgc
     3841 cgccttcaca aactgctgga tgtcgcaccc catattctca atgtccaacg agaaggcaca
     3901 cacgtaaaag ccgttccgcc tttcttccgt gtcgatggga ccaaacttga tctgcggtag
     3961 cggcatgagg gcatccatga ggatgtgggc gttgcgacgt cgccgcggca tgttccaagt
     4021 atccatccga tccagctcgg ccaatccgat gacggcctgc atctccgtca tgcgataatt
     4081 ccagcccaat cgcgtgtgga tgtaagggag tttttgctcc agctccagga gattgagacg
     4141 ctctttcacg tcgtaaccgt gatcccgaaa actgcgagcc tcccaggcca ggtcttcgtc
     4201 atccgtcgtt accatccccc cttcgccgcc tgtggtaaag gtcttgtttt ggcagaagct
     4261 acaagccgcg atgtggctca aagtccccgt ctttttgccc ttgtactcgc ccccaaaggc
     4321 ctcggcattg tcttcgatca cgaacagatt gtgtcgtttg gcaaaatcgt tgatcttatc
     4381 catgtcgcac acattgccgt acaaatggac gggaatgatc gcccgcgtcc gctcgttgac
     4441 aagcttttct gccgattcca cgctgatgca gtgatcttcg aggttgacat cggcgaatcg
     4501 tgggatggcc ccggcctgta agacggcaaa actggtggcg ataaaggtgt aactgggaac
     4561 gatgacctca tcacctggcc caatgccaag ggcggacaag gccacgtgca aggcggcggt
     4621 cccggaggaa acggcgatgg caaagcgact tccctgccat tctgcgaagc gcttttcaaa
     4681 ggccatacca tggggacccg tccagtaatt gacgcgcccg gatcggagga ccgcctctac
     4741 ggcgcgaatg gcttcttctg agaactgtgg ccaaccaggg agcttgttcg tcaccgcccg
     4801 cggaccacca tcaatcgcca acttttgtga gacaatgtaa tcgaacgaca tcacagcacc
     4861 tcatgcgtta gcacaagacc acttcccgaa acgaccctgt cgctttcttc catcggtcct
     4921 ccctgcctca cgactagcca acggcaagag atgttttagc ccacagagat ggtcattgca
     4981 agtgttccgg aagcgaagcg ataagaggtc cagaaaagcg cacaacctgg gcgatggggg
     5041 cgagagttaa ctcgccccca tgaggccgtt ccctgattcc ccaggtcctg cccccgtagc
     5101 ctgccctcgg gtcaataaac ccgctgcccg tttcggctcg ccccttaaga agttgcccct
     5161 tcaccccctt agtctgcccc agggccgtgg cggcagtgcc taacaacgca gcccttcgct
     5221 atttacccct ttttcaaagc ctgccccgaa agccctcttt cctccacaca tcacccctct
     5281 cggcacgccg gcgcacggcc cacttgcccc ttctctgtcc cggccccgcc tttcctgccc
     5341 cttctgcgga aacccacctc gctggcatca ctcaccagcc tgccctgctt gcttcctact
     5401 cttaggatat ccaccacccc ggacaaccca tgtccaacct ggaaacggtt ttttcggatt
     5461 gcgaaccgcc tttcggaaac cccgatttcc gggaggatgg tccgtggctt tttctgccgc
     5521 aaacttttct ttttgacttc cttcacggtc ttccgccggt tttttatcat cccgtctggc
     5581 aatggacctg cggcgaggac acaataatag acggtcgcca gatgcggtgt cccgtttttt
     5641 cccgacgtgg caaacagtta aacgtcgcga cagcgtcggc caacccagtg tgatcccaaa
     5701 attgaaggag ggagcgatga cttaccagga atctgatcaa gcgttcgagc cagaggccgg
     5761 tgcacaagcc ccgtcgccgg gtcaggaagc accaaggagc gcgcaggcgc agcctgatgc
     5821 catcagcggc caatacgagc gcccgccacg tcgtggcgat cccgatatcg agttgtggaa
     5881 aggcggctat tccatgcggg cgatggtggc cacgtggatc ctcctcggct tgatcaccat
     5941 cgtggttcta gttctcgatg tttggatcac gcaaaacttt ctcaaagaac atcaagccct
     6001 tctttgggaa atcaccctgg gcttcctggt cctggtgtgg ggctacttca ttttggtcgg
     6061 cctatacaag gcagcgacga ttcgatatcg gttaacgacc taccggttct atcaccagaa
     6121 gggcctgatc tttcgcacga tcgactctat ggaagtcatt gatatcgagg acctcgcgct
     6181 ccatcagaat ctgttggaac gcttggtcaa tgtgggccgc attctcctga gaaccaaaga
     6241 cccttctgat ccccttctca tgctctatgg ggtgtatgat ccgcacgagg ccttcaagcg
     6301 gatcgacaag gcacgccgcg atgaacacgt ccggcgcagc atcaagattg gctaaggggc
     6361 ttccgtccta aaccggtgcc gcgaccatag gtacgagcgc cggtgatgat cgtgtgacag
     6421 acaacgcagt gacaacaagg agactgagac atgaaacgac gaacatttct gctgacactt
     6481 gtagttatca gtttaagtgc cgttcccgta ctggccgcac cgtataaggc cctcattgtg
     6541 acaggccaaa atgcccacga ttggaaaggc acgacgcccg tcctcaagca acttctcgaa
     6601 gaaacgggcc tattcaccgt ggatgtggcg caatcaccgg ccaagggcga ggatatgagc
     6661 tcgttcaagc cggagttcaa gaattaccac gtcgtggtgc tcaactatca gggtgattcc
     6721 tggccggaag aaaccaaaaa ggcctttgtg gattacgtca agaacggtgg cggagtggtg
     6781 gtttatcact ttgcctgtgc ggccttcccc gattggccag agtataacga gatcatcggc
     6841 ttgggcggct gggggaatcg caacgagaaa tggggtccct acgtccgctg gcgtaatggc
     6901 cgtgttgagt ttgacttctc ccctggtcgt ggcggtggtc atgggccgat gcaacccttc
     6961 gccattgatg tccgctatcc agaacacccc atcatgaagg gattaccgcc acggtttatg
     7021 cacgttcccg atgaacttta cggctggctc cgcggtccgg caaagaactt gaccgtgctg
     7081 gccacagcct tcgcccccaa ggacaagggc ggttccgatg agcacgaacc gatcctattt
     7141 acggtggagt acggcaaagg acgtgtcttc ttcaatgccc tgggacatac agcaaacgaa
     7201 ttgaagagcg tggcctttat cgtaacgtat cagcgaggcg cagaatgggc ggcgtcaggt
     7261 aaggtcaccc aacccgtgcc tgacgatttc cccacggcgg atgaggcccg ggttcgccca
     7321 tagcgccgac ataccctggc ttttggtttg aaaatagcga acataggtgg gcgaggagac
     7381 aatgttggcc tcgcccgccg ttttgtgctt tggggtgaga ccatggacgg gggcttatcg
     7441 cacggaaatg tagttgggta gcttttgaaa agtcgttcga tcgattgctc cgcggttttt
     7501 gggggtagga gaatgagcca tgacgcgacg gcggcaagac tttgggcagg atcaaaatga
     7561 gatcgctcga ctggacgatc tccttcgccg agatcacacc ctcatcggtg gagtgctttt
     7621 tgggcttcag ggacaccttg tggaggtcca agcccgcgcc cttgaagtcc tcagacggag
     7681 tatcccctgg cgggaggccg ttaacatttc gggcatggca aaccttgccg tacgggaatc
     7741 gctcaaccgg atatcgggag cgttcgctaa atatgcaatc ccggaaccgc aggtggcaat
     7801 tcagatcaac ctcgcgccag ccggtatatg gaaggaaggc acgtggctgg atttgcctct
     7861 ggccatcatc atgctccagg catcgggcta cctgccggac ctccccgaaa cgatcgaagg
     7921 cgaatacgtc ctggtcggag aagtgggctt acatggcgaa attaggcggg tgccgggcgt
     7981 cctttccatt gctgaggcgg caatgcccgg acaaaccctt gtcgttccca gcggaaatga
     8041 acgggaatgt gccctgatca ctgccagatc tgatcgtaag tcgtgccgcg tggccgccgt
     8101 ctcaactttg gaggaggtga ttgaattctt ccaaggaaag cgaaaactcg aaaacgtcct
     8161 caaagaggag attcgtttcg agaaggtagg ggaaaaaccc ctcgattttg ggcgaatcaa
     8221 gggacaagag cgtgccaagg aggcagccgt cattgccgcc gccggagggc acaatctcct
     8281 cctcatcggg ccgcccggtg aaggcaagtc cacattggcg cgggcgatgg ccggaataat
     8341 gcccaagcta acaatcgacg aaaaggttga gctgacgcga atttactcgg cggccggcct
     8401 tttaaagaca gacggcgttg ttgtctccta tcgtcccttt cgggctgttc atcacacggc
     8461 gagcaaacag gcgctggtag gcggaggaac cgatgtcatc cgtcctggag aggtcacact
     8521 tgcccacttg ggcatccttt tcctcgacga acttcccgaa tttcgccgcg attctttgga
     8581 agccttacgg gaacccatgg aaacgggcga gatcacgatc tctcgcgcca aggccagggg
     8641 cacctacccc gcccggttca cactggtcgc cgctatgaac ccctgtccct gtggctatta
     8701 tcccagtcct gcgtgcacat gctcgcggga aaaggtcaag gcctaccgga gtaggctcag
     8761 tggtccgcta cttgaccgca tcgacatgca ggtggaaatg gagcctttga gcctcgagga
     8821 aaagctcgcc aaaacccaag aaggagagac tccccgtttg cagcgactgg ttcaggctgc
     8881 gcgggatcgc cagacgcgtc gctttgcggg gacgtcgatc ccttataatg cggccattcc
     8941 ggccggacgt atccaggact tctgccaatt ctcatcgaac gggttcgaac gtttcaagca
     9001 gatcgtcgaa gaaaaccgga tgtccacccg tcgcaccgat cggttggcca aagtcgcctt
     9061 gaccgtggcc gacttggaag gtgccgacaa catcgaggcc caccacgtcg ataaggccgc
     9121 gtcatttgtc atcggcgatc gcgacatcca ccgagattga ccgccgtcaa atcaccgcct
     9181 caggacccac ggccatgatc agtcgcattg tcccgcttca tggccccgat gtggggtcag
     9241 ccgcggtttc tccgccttct cttttcaccg attcggaagc gtgaaacctc ttccgcccaa
     9301 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagacaccc aagggcagca
     9421 tgagcaagtt gacgaagggg atcgtccagc tctcgtaaag ggcggccaca cagaggaaaa
     9481 tcacgaggat agagaaggca taaagaatgg gggcctgtgt ggtggccatt cgttcttgat
     9541 aggaaatacc tcgccaatca aagcccaccc ctggtggtag ttttcctacg agttcctcca
     9601 tggcccgcat ggcttcgccc gaactgtggc cgggagccgg ttcaccccag atgttcacag
     9661 cgggaaagcc gttgaatcgc tctaatcgcg gtgagccgct tgtccagcgc gtggtcgcga
     9721 aggagtcgaa gggaaccatc ttcccttctt ggttgcggac gtacagtctt tgtagatcct
     9781 ctgggagcat gcggtaaggg gcatcggcct gcacatagac acgcttgaca cgcccacctt
     9841 ggatgaaatc gttgacgtac gaacccccaa aggccgccga gatcgtgtta tgaatcgagg
     9901 taatgggaac gccgagaatc cccgcccgtt cccaatcaat atcgacgcgg tactgggcaa
     9961 catcctccat gccattgggt cggacccgct gcaaaacggg atgctgggca gccatttcca
    10021 gaagttgatt gcgggcggcc atgagggcgt catgcccaat ccctcccatg tcgatcagtt
    10081 caaaatcaaa ccccgtggcg tttcccagtt ctcggatcgg cggtggcgga aagacgtaga
    10141 tactagcttc ccgaattgag gagagagccg ccgtggcccg atcggcaatg gcctttgccc
    10201 gcaggtcctt ccgctctcgg aggtgccagt cctttagcat cacatagacc atgctgttgt
    10261 tctgggcacg ccccgaaaaa ccactcccca cgatggtcat gcacgacacc acgccttctg
    10321 attctttctc caggaagtat tgctggacct tgtcggccac ggcctgtgtc tgctctaacg
    10381 ttgatcccgt gggcagggta atttgggcca tcagcacgcc ctggtcctct tggggaataa
    10441 agccggtggg caaccgcaag aagagatcca cgatcaacac cagaatcaac acataaccca
    10501 ccaaataggg aactttcttg cggatcgaat gatggacgac cctcacatac tgatcccgaa
    10561 tccagaagaa gatgcgatca aaacccacga agaaccatcg caaaaaccag acggcactct
    10621 cgcccggctc atgtcctttg gcaatcggct taagaagcga tgcgcagagg accggcgtca
    10681 gaataagggc caccaccaca gaaagcaaca tggccgaaat aatcgtgacc gaaaactgcc
    10741 gatagatgat tcccgtcgat cccgggaaaa aggccatcgg cccaaagacg gcggagagga
    10801 ccagaccaat gccaatcagg gcactggtaa tttgacccat ggacttgatg gtggcctctc
    10861 gcgggggaag gccctcttcc ctcatgatac gttccacatt ctccacaaca acgatggcgt
    10921 catcgacaag cagcccgatt gccaagacca tggcaaacat ggtcaacata ttgatggaaa
    10981 agccggcggc tccgaggaca gcaaacgttc ccaagaggac cacgggcaca gcgattgtcg
    11041 ggatgagcgt cgcccgcaag ttacctagaa acagcagcat cgccagaaag acgagaccta
    11101 ccgcttcaaa aagcgttttg acgacctcaa agatggccac gcgaatgaaa ggcgtggtgt
    11161 cgtaggaata gacgaccctt acaccgggcg ggaaatagtg actcaattcg gcaaggcgtt
    11221 tcttcacgcg atcggccgta tccagggcat tggcccccgg ttccaatcgg acggccaagc
    11281 cggccgatgg cattccatta aactgaatat tgctagcgta ggagtccgta cccaattcca
    11341 cccgcgccac atcgcggatc cgcacgatac caccatcggg ctggacgaca atgggaatgt
    11401 tggcgaactc atccggcgtt tggaggtggt tctgaaccac gatgctgaca ttgagccttt
    11461 ggcccggaac ggcgggggcg cccccgaact gtcccgcgga gacttcgaca ttgtaggcgc
    11521 ggacggcccg aatgacgtct tccacagtca actggtaggc ggtcaatttc tccggattga
    11581 tccaaatccg catggcatac tggctgccga agacctccac ctcgcccaca cccggcacgc
    11641 gggccaagac cttttccaaa ttggattggg cataatcgcg gaggtcgatg tttccgagcc
    11701 gcccgtcctc gctgataaga ttggcaatca aaaggtagtt gcgggttgcc ttgcggaccg
    11761 taattcccat ccgctggacc atctccggca aactggccat cgccagttgg agtttgtttt
    11821 gaactttagc ccaggccaga tcgggatcgg tccccggggc aaacgtcagc tcaatgttgc
    11881 aagaacctgc cgagtcgctc gtggcggaaa aatagatcat gtcatcgagg ccggtcagtt
    11941 tctgctcgat aatctgcgtc acgctgtttt ccacagtctc ggcagagccg cctggataaa
    12001 acgccgtaat tcggatggta ggcggagcga tatccggata ctgcgaaatc gggaggttgt
    12061 agatcgccaa cccacccagg accatgatca tgatggcgat cacccaggcg aagaccggtc
    12121 gatcgagaaa aaatcgggaa atcacggtta cctcccgttg gctgactcag accctcgtga
    12181 tgattcctgg gatgccgcag cggatggttc gacccaaggc acaacattga cgaccatatc
    12241 cggcttgagc ctctgcatcc cttccatcac gattcgctcc cccgccttta atccctctag
    12301 taccaaccag cgatgaccaa aggcacgatc ggtgacgatc gaacggacat gaaccttgtt
    12361 ctgatcatcc accacccaca cgaaaggctc ccctcgcgag tcccgttgaa cagcttgttg
    12421 gggcacgaga atcgcatcct gctgctcgcc tagctcaatg ctggcgcgga caaacatccc
    12481 cgggagtaat tccccctcgg gattgggaac gatcacccgc acgatcacgg actcggtgcc
    12541 cggatccaca ctgacatcgc gaaatttcag cgttgcgtca tgaggataga tagtgccgtc
    12601 ctcgaggatg atcttgacct tatcggtacc ctcggccttc agtcgcccct gctgcaatgc
    12661 ccgacggaga cgatacagtt ggctggtcga ttgcgggata tcaacgtaaa tggggtcaaa
    12721 ctgctgaatc gtggcaagag gtgtcgattg ataggccgtc acaatggccc cctctgtgac
    12781 gcgggatcgt ccaatccgtc cggaaatcgg tgccttcacc tgggtgtagt cgagattgat
    12841 cttggccgcc tccagcgcag cctcagcctg ctggacggca gcctccgctg cggccagggc
    12901 cttccgatcg gtttcgacgc gggctttggc cgcaagcaac gcggcctcgg ccacctcggc
    12961 atttgtcacg gcttgatcac gctccatggc cgtggtcgct agctgttcgt acatccgttc
    13021 gtagcgctca cgattcgtgc gagcaaggtg aaaaagggcc tcttgttctt tttgcgctgc
    13081 catgctggcc tcaagggcgg cccgggcctt ttccacggct tgttgggccg ttttcagatt
    13141 ggccaacgca acgtcgtaag ccgcctgata aggcgccgga tcaatttgat acaacacatc
    13201 tcccgccttg acttccgagc cttcctcaaa aaagcgtttc aaaatcaccc cgttgacctg
    13261 cgggcgaatc tccgacacca aataggaggc cgttcgaccg ggtaactccg tcgtgatgcg
    13321 gaccttggtc gtctccacgg tgtaaacggc aacttccgga ggtcctgggg gcggcgctgc
    13381 ggatggcgcc gtctcacagc ccgtgatcaa gagaatatcc aaaacaattc cgatgatggc
    13441 tacaaatctt ggcaggatgt tcatgaagac actccacgcc gcggcgaatc actcgatctc
    13501 ccggaagcga ttcatcatcc gcccgtcaat aaaccaacga acgtcctacc caacatcgtg
    13561 ttcatcctaa gaaacccccg aagcgatctc gtgttagcgt gttctgggcg ggaatctgat
    13621 ttcatcgggc aggtcgcgcg cttttagtat agttacgacg agcttgaaat ggcaccctct
    13681 ttggcaaatg cgac
//
LOCUS       DAOSUT010000108        13685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2688_length_13685_cov_9.600220, whole genome shotgun sequence.
ACCESSION   DAOSUT010000108 DAOSUT010000000
VERSION     DAOSUT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2688_length_13685_cov_9.600220"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLD05_10550"
     CDS             <1..575
                     /locus_tag="PLD05_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77922.1"
                     /translation="LTMTAKAVVGVRADTGKLLFRHPHETAYDVNACMPLYADGVVVV
                     STGYGSGSEAIRLTRRGDTVATQQVWSNRELDNHHGGVLLFNGFIYGSSYNGRWLCLD
                     WEKGESRYADKGVGKGSLTYADGMLYTFSERRMVGLVKVDPTKHELVSSFRLPSEERD
                     PSWAHPVVCGGRLYLRHGNLLFAYDVRKRP"
     gene            812..>1317
                     /locus_tag="PLD05_10555"
     CDS             812..>1317
                     /locus_tag="PLD05_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP77923.1"
                     /translation="MGNIYIGANLEFVRHADKSFEWGVQKAAELGYEYVEPMVHWGRE
                     LMSEAGYFHSVSMLDDPYRIRNTCEKAGVKISGLSAHCPLGRPEVSVEYLKQAIRYAA
                     ECGAPVVNTDEGPKQPWTTEEEDFVLMRYTLREATFVAERRGILIGLEPHQQYSKSPD
                     GLDRIAALV"
     assembly_gap    1318..1417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1442..2992)
                     /locus_tag="PLD05_10560"
     CDS             complement(1442..2992)
                     /locus_tag="PLD05_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP77924.1"
                     /translation="MRRLAFLLIGTFFAHLGLFGVCEADDQPQWGERFSRNMVSREKH
                     LPADFDPATGKNIKWAVELGTNTYSSPVVAKGRVFIGVNNAKPRDPRHQGDRAILMCL
                     DEQSGELIWQLVVPRLGEDDPYLDWPRISMCSPPTVEGNRVYTVTNRFEVVCLDLEGQ
                     ANGNDGPYTDEGRHMVPPGEAPLEVTNLDADIIWLFDLPSQAGIYPHDGAHVSILLDG
                     RFLYLNTGNGVDNTHRKIRAPDAPSLIVLDKETGRLVAKDDEKIGPRVFHSTWSSPAL
                     GVVDGQRLIFFCGGDGVVYAFRALPQDWQSTEVVPLERVWKFDCDPTAPKENVHQYIT
                     NRKVSPSNIKSMPVFYRDRVYVTVGGDIWWGKREAWLKCINARGKGDITSTHELWSYP
                     LKEHCCSTPSIAGDLVFVADCGGFLHCVDAETGHAYWTHDLRGETWGSTLVADGKVYV
                     GTRRGVFWTFAASREKQLLAEINLRDSIASTPVAANGVLYVTTLTHLYAVQQGPDVSE
                     KTSSGMAN"
     assembly_gap    3112..3211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3212..5293
                     /locus_tag="PLD05_10565"
     CDS             <3212..5293
                     /locus_tag="PLD05_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOP77925.1"
                     /translation="RSYGFVGLGPICYVVLAICFSLEFASVMEDPSQTEHFADKMTHR
                     SIRREYDRSAKQVVWLVVLIWSSVILASLWGMFLADPMVRSIQGAVSLALLWSAGLGV
                     IILFGYRWADYRTRIDQTMTRQIAFRLFTRDTPAAVALVDAYGRFRLVSPAFCQLLGR
                     TEKELIGQRAIRFAHPDDAAILREQYRLRRRGQSSTYQLRVIRADGAVRWLLAAVWPV
                     YDDKGRYCGGLGLVADITEQKQAEEELRQHTRELESLVQQLEKAQAEARAAALAKTEF
                     LATVSHELRTPLTAILGYAEMLLLEGDLTKIPANRLNAIQTILRNGEHLLQIINDLLE
                     MAKLETGRLEPEVRDFSLPQLLHDVVRLMQIRAESKQLALRLEICGPIPKMIRSDPLR
                     IRQILINLVGNAIKFTELGSVRIVVQVPRNEDGTCSFVCEVIDTGMGIPPEPCDKIFE
                     PFHRGDPTSRRKFSGTGLGLTVSRFLARRLGGDITVVSEVGLGSNFRLVIPLGLADQY
                     EWITCTADDLDVTQKSIREVQAVASAEKGAPLTARVLLVEDCPDTQRLFTHILSQAGA
                     DVTSASTGRDTIRLAVQAMVEQRPFDCLLLDMQLPDIDGYHVARMLRDSGYTAPIVAL
                     TANMTPGERERCLASGCDDFATKPIKRQDLVNLVAKYAPQTGGLAPIPGEMSSRPSLT
                     TGAPLPQPASN"
     gene            complement(5332..6591)
                     /locus_tag="PLD05_10570"
     CDS             complement(5332..6591)
                     /locus_tag="PLD05_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOP77926.1"
                     /translation="MTTVTPGQELQASQGQTSGTAAQWEGYLRADGRKGIRNCLVVVY
                     LVECAHHVAAEIASPFANRGVELIGFPGCYPSEYAHKVLSRVTTHPNVGAVLLVSLGC
                     ESFDRARLRAEIAASGRPVELLVIQEIGGTARTIKAGRQWIKSVLPELAKVPRCPMEL
                     RDLVVGTICGGSDATSSLTANPACGLVFDRLVDSGATAIFEETGELLGCESLLAERAA
                     RPELADVLRACVEKAARYYATLGHASFAPGNTEGGLTTIEEKSLGAYLKSGHRPITGV
                     LKPGDLPPSPGLYLLDVVPDGEVRFGFPNIADNAEIVELIACGCHLILFTTGRGSVVG
                     SAISPVIKICANPETYARMKSDMDINAGEVLTGKSTLEEVAERIWQFMIAVAGGKQTV
                     SEALGHREFILTYKTFEPLGPACLPTV"
     gene            complement(6670..7233)
                     /locus_tag="PLD05_10575"
     CDS             complement(6670..7233)
                     /locus_tag="PLD05_10575"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOP77927.1"
                     /translation="MKPKLLRLHPRDNIAIATAPIKAETVLKLDRVKIQIISNVPMAG
                     KVATRPIAEGEKIIKFGQPIGHATRNIEPGEWVHTHNLASDYLPTPERPSPASDVSTQ
                     PVSEPVGENSAVGTSAVVETLSGPSAEGEISANSKAFSSDSLPSQDSPVVEEGPTPSP
                     TPNEPNEPKLEQGTSPAEIERSDSTVQ"
     gene            complement(7438..7899)
                     /locus_tag="PLD05_10580"
     CDS             complement(7438..7899)
                     /locus_tag="PLD05_10580"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOP77928.1"
                     /translation="MQRRTREKAVKPHRLNYLARAMDKHSRRYFDRILEEVIEELPQL
                     VRDAMDKVPLYVEDYPSRRVMRQTGIRNRRELWGLFTGVPLNEKHFDAPSVTLPDRVT
                     IYREGLLWSAVGRDGYVDEKRLKKQIRITILHEYGHYYGMTEEELEQYGYG"
     gene            8048..9082
                     /locus_tag="PLD05_10585"
     CDS             8048..9082
                     /locus_tag="PLD05_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77929.1"
                     /translation="MLKSEVARNYPFLRPAFSRRGFVTFKFAEGVKPDEGFSLEAVFA
                     RTWGFCFGRVPASDWQVAANEVWAKVANIPVGWIHVWPRDTSPPGRSEPEKPWPAEIS
                     QIQQVLMEHAPTKAIPPNVSQESTPCPPGVWVLDCVVVEPTEWWIGIHRAGSLESRYP
                     GGRFPIVLPEYAVSRAYLKIEEALRWSGFKIPANARWAELGSAPGGASQALLDRGYEV
                     LGVDPAEMHPLVLSHPKFTHLRRRTPQAPRRAFRKVRWLAADINAAPNYTLDAVESIV
                     TYPQVNIRGMILTLKLPNWQLAENIPEYMERIRSWGYNIVKARHLWNNRQEVCVAALM
                     KPFRRKPSHS"
     gene            9361..11829
                     /locus_tag="PLD05_10590"
     CDS             9361..11829
                     /locus_tag="PLD05_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77930.1"
                     /translation="MEFATPCFHVSFYVAILVGWLLPSIGAGLEPGLYPSTRDYPFTR
                     AMAPDYPNLPSLPTPGDNSRSATVTSSRLEQAGDDAEDAIIEGPDMALESADSQSAGA
                     MVREEGTKPDDEGLEKQDVMEAPQSPDGPSAAPQKPNADGDSSTDKPSARRSTIDPTS
                     VGRPLAAGIAKLVAPEIESQMEGANITARFQQWRSYAASRLAATRADYTSSEINGMAR
                     LKWYEHLLLNPLNVPHETEEFTRLLHAGLTAKSSEGFRFALRQARSKMDVPDGSLPGD
                     LETLWKSENNDPYKKLENILVTARKTYARTVEPLTPAELNELAQQLNYVLTTNTIVGH
                     TVDSRSSAMRMVRLLQKMDRSAQWDLAEVLVAVCDKEFLDSLRTSAPKGGAGVAVAGV
                     TGEVLRRIDTPAGTILVGGPGKNEYRLEEMKDVAAIVDVGGDDVYLEGTVSVERPILI
                     IIDLAGNDRYEGKLPGIQGSAIIGGSILVDCAGNDIYWGQDVAQGSALGGVGILVDMA
                     GDDQYLGYRRVQGQAIGGVGILLDYGGRDDYRAAMWAQGFGGPLGFGLIDDLDGDDHY
                     YVGGRFYDSYPETPGYDGWGQGVGAGIRGAANGGIGVLLEGNGDDVYEFDYFGQGGGY
                     WLAIGLARDFAGNDQRLGATLNAYNRSNRTQQRFQRFGNGWGCHYAVGVLIDDLGNDS
                     YDGSIMGTGFGWDLSVGYLLEMSGDDQYLARVGGVQGQGAQASLGVLYDFEGKDVYRG
                     TSQGYASSSISYHPYPTCGGNFSFLIDYGGEDEYGSRVRNNAVSRRGMGGFVVDRPKQ
                     EELDAMHASAKTSISSPISNSSGQ"
     gene            complement(12018..>13685)
                     /locus_tag="PLD05_10595"
     CDS             complement(12018..>13685)
                     /locus_tag="PLD05_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77931.1"
                     /translation="GLPEVVAQPSQTKPVLVVSFSGLDRLWEDADFLGKLAGVSNLSD
                     NVKQILAAQTGGVPGVPGVDAKRPLGLVVQTNGADFIVAGYLPVVDLQKMLDWLQTRG
                     VEPEKQGTAYRVDMPNGQSLFLAQKGDWAVISVSPEALDQASANPPQELDQLASIYDL
                     GVAAYIGNIPPMWKQLGLAMIQQGVQTAMEQQPDQTDEQFAAQKAMIQRSFEQMQQAI
                     NEMDRILIGVQIDAAASVIGLDIETTILPGTQSAQELALNKNLKTKLAGFIASDATAA
                     LRETVKAGPRSITQFKDSLSQYESMISSAFDQDTKLKPREKKLLKQLFSTFFKSLAET
                     VEKDQLDIAMSVKLDSKSCEGLMAMTLVNPEEVENTVAEILKQAAKDNPDFAKVLKLN
                     AEMYKGVRLHVVELPADALKEAPQALKDIFGSNAGAVIGFSENLVCMAFGPNPVSILK
                     NALDQSASEVAVDKIFEFSLAVKPLIEFVQVAASEEWGEEERQLAEQLLASAGTKDRV
                     TMDGVLIENGSRVRIEFQEGVLKVLAQLVIKAVAQQGVGFAPGAMAP"
BASE COUNT         2918 a         3661 c         3845 g         3061 t
ORIGIN      
        1 tactgaccat gacggccaag gccgtggtcg gggttcgggc agatacggga aagctcctct
       61 tccgacatcc gcacgagacg gcatacgacg tcaacgcttg catgcccctc tacgccgacg
      121 gtgtcgtcgt ggtcagtacc ggttatggaa gtggatcgga ggcgattcgc ctcactcggc
      181 gaggagatac ggtcgcgacg cagcaagttt ggagtaatcg cgagcttgac aatcatcatg
      241 gtggtgtcct cctgtttaac ggcttcattt atggatccag ttacaacggg cggtggcttt
      301 gcctggactg ggaaaaaggg gagagccgtt acgctgataa gggggtggga aaaggctcgt
      361 taacttatgc agatgggatg ctttatactt tcagcgaacg aaggatggtc ggactggtga
      421 aagtggatcc gaccaaacat gagttggtca gtagctttcg gctcccttcc gaagagcgag
      481 atccatcctg ggcccatcct gtggtctgtg gtggccgact ctatctccgg cacgggaacc
      541 tcttgtttgc ttatgacgtt cgcaaacggc cgtagtggat gcagaacgtc acgcttggtg
      601 acaaaggggt tcgtctcgcg gtttccgatg aattgacctt catgagatag tattcgcagg
      661 gatggcgagc gcgtggccct ccaacaaatc gcgcgggcac tcggtattca gttacttgcg
      721 gccgatggat atctgtgatt taatggccgc aacgtaacca acgcattgcc tgcgtagttt
      781 ggggacctct ttttccgagg agggtcaagc gatgggaaac atctatattg gtgccaatct
      841 tgaattcgtc cgacatgccg acaaatcgtt tgaatggggc gtccagaagg cggccgaatt
      901 gggctacgaa tacgtggaac ccatggtcca ttggggccgc gagttgatgt ccgaggccgg
      961 ttatttccat agcgtatcca tgctggacga tccttatcgc atccgcaata cctgcgagaa
     1021 ggcgggggtc aagatttccg gcctttcggc ccattgccct ctcggaaggc cggaagtcag
     1081 tgtggaatac ttaaagcagg ccatccgcta tgcggcggag tgtggtgccc cggtggtgaa
     1141 taccgatgaa ggccccaagc aaccgtggac cacggaagaa gaggattttg tcctgatgcg
     1201 ctataccctt cgtgaggcca cattcgtggc ggaacgacgt gggattttga tcggtttgga
     1261 accgcatcaa cagtacagca aaagccctga tggcttggac cgcatcgctg ccctcgtnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat caccgcggag ggcccttccc
     1441 attagttggc cattccggag gacgtctttt ccgacacatc gggaccttgt tggaccgcat
     1501 ataagtgcgt caatgtggtg acgtacaaaa cgccattggc agcgacgggt gtggaggcga
     1561 tggaatctcg aaggttgatt tccgctaaaa gttgtttttc gcgagatgcg gcaaaggtcc
     1621 agaagactcc acgacgtgtc ccgacataaa ccttcccatc cgcgacgagc gtggatcccc
     1681 aggtctcacc ccgcaggtca tgcgtccagt aggcatgacc tgtttctgcg tccacgcaat
     1741 ggagaaatcc cccgcagtcg gccacaaaaa caaggtcacc agcaatcgac ggggttgagc
     1801 agcagtgttc cttgagggga taagaccata gctcgtgggt ggacgtgatg tcgcccttcc
     1861 cgcgggcatt gatgcatttc agccacgcct cgcgctttcc ccaccaaatg tccccaccca
     1921 ccgtcacgta aacccggtct cgataaaaca ccggcatgct cttgatgtta ctggggctaa
     1981 ccttccggtt agtaatgtat tgatgaacgt tctcttttgg cgccgtggga tcgcaatcga
     2041 acttccacac acgttccagg ggaacgacct cggtggattg ccaatcctgc ggcaaagcac
     2101 ggaaggcata gacgacgcca tcacccccgc agaagaagat caagcgttga ccgtcaacta
     2161 ccccgagtgc cggtgaggac cacgtggagt ggaaaacccg tggcccgatc ttttcatcat
     2221 cttttgccac gagtcggcct gtttccttat cgaggacaat cagactcggc gcatctgggg
     2281 cgcgaatctt ccgatgcgta ttgtccacac cattacccgt gttcaggtag aggaatcgac
     2341 cgtcgaggag gatggaaaca tgggcaccat catgagggta gatgccagcc tgggaaggga
     2401 ggtcgaaaag ccagatgata tccgcatcca gattggtcac ctctagggga gcctcaccgg
     2461 gcggcaccat gtgacggcct tcgtcggtat atggcccatc attgccattg gcctggccct
     2521 caaggtccaa acacaccact tcaaaccggt tcgtgacggt gtaaactcgg ttgccttcca
     2581 ccgtgggggg agagcacatc gagatacgcg gccaatccag atagggatca tcctccccaa
     2641 gtcgaggaac gacaagctgc cagattagct ctccggactg ttcatccaag cacatcagga
     2701 tcgcccgatc cccttggtga cgtggatcgc gcggcttggc attgttgacc ccaatgaaaa
     2761 cgcgaccctt ggccacaacc ggggaggagt acgtgtttgt ccccaattcc acggcccatt
     2821 tgatgttttt gcccgtcgcg ggatcaaaat cagccggaag gtgcttttct cgcgagacca
     2881 tgttccggct aaagcgttcc ccccattggg gttgatcatc cgcctcgcat accccgaaca
     2941 acccaagatg ggcgaaaaag gttcctatca acaggaaagc taaccgacgc atgcttgcgt
     3001 ttggccgagt aatggcagga ggaagatttc gcatgacagc gactctctga atgtctgaca
     3061 cgaactgatg gcaggaaagg cttggggatc gtctttggct tgtcgggaac tnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagaagctat ggtttcgtag gtttgggccc
     3241 catctgttat gttgtcttgg ccatttgttt cagcctggag tttgcatcgg tgatggaaga
     3301 tccaagtcag actgagcatt ttgccgacaa aatgactcac cgctctattc ggagggagta
     3361 cgatcgttcg gccaagcaag ttgtttggct ggtcgttctt atttggtcga gtgtgatttt
     3421 ggcgagtctt tgggggatgt ttctggccga ccccatggtc cgctcgattc agggggcagt
     3481 atcgctagca ctcctttggt ccgcaggttt gggggtgatc atcctgtttg ggtaccgctg
     3541 ggcggactat cgcacccgga ttgaccaaac gatgacccga cagatagcct ttcggctctt
     3601 tacccgggat actccggcag ccgttgctct tgtggatgct tatggccgtt tccggctcgt
     3661 gagtcccgcc ttttgtcaac ttctcggtcg tacggaaaaa gagctaattg gccaacgggc
     3721 tatccgcttt gcccatccgg atgatgcagc gattcttcgt gagcagtacc gactgcgacg
     3781 ccgtgggcag tcatcgacct atcaactgcg ggtcatccgt gccgacggtg cagtgagatg
     3841 gctgctggcc gcggtgtggc ccgtttacga cgacaaaggc cgatactgcg gcggtcttgg
     3901 cctggttgcc gacattacag agcagaaaca agccgaagag gaactgcgac aacacacacg
     3961 cgagttagaa tcgcttgttc aacagctgga aaaggctcag gccgaagccc gtgcagccgc
     4021 cttggcaaag acggaattcc tggctacggt cagtcacgaa ctacgcacgc ccttgacggc
     4081 catcttgggc tatgcagaga tgcttttact cgaaggtgat ttgactaaga ttcctgcgaa
     4141 ccggctaaat gccatccaaa cgattcttcg aaacggcgaa catcttctcc agatcattaa
     4201 tgatctgctg gaaatggcga agttagaaac tggccgattg gagccggaag tacgagactt
     4261 ctcacttccg caactgctcc acgatgtggt tcgcctgatg caaatccggg cggaaagcaa
     4321 acagttggcc ttgcgactgg aaatttgtgg accaatcccc aagatgatcc gcagtgaccc
     4381 cctacgaatc agacaaattc tgattaacct tgtgggaaat gctatcaagt tcacggagtt
     4441 aggatcagta cggatcgttg ttcaggtgcc gagaaatgag gatggtacct gttcctttgt
     4501 gtgcgaggtt atcgatacag gcatgggtat cccgcccgag ccttgcgata agatcttcga
     4561 accgttccat cggggtgacc cgacgtcgcg gcgaaagttc tccggtacgg gcttgggcct
     4621 gaccgtcagt cgctttctag cacgacggct gggtggcgac attaccgtgg tgagcgaagt
     4681 gggtttgggg agcaacttcc gattggtcat acctttgggt cttgccgatc agtacgagtg
     4741 gatcacctgc accgcggatg acctcgatgt tacccaaaaa tccattcgtg aagtccaagc
     4801 cgtagctagc gcggaaaaag gtgcgcccct caccgccagg gttctgttgg tcgaagattg
     4861 tccagatacc caaagactgt ttacccacat tttgtcccag gccggtgctg atgtgaccag
     4921 tgcctcaacg gggcgagata cgatccgctt agccgttcaa gccatggtgg aacagcggcc
     4981 ttttgactgt ctcctcctcg acatgcagtt gcccgacatc gacggttacc acgtcgctag
     5041 gatgctccga gactcaggat acactgcacc catcgtcgcc ctaacggcga acatgacccc
     5101 cggcgaacgc gagcggtgcc tggcctccgg atgcgacgac tttgccacca agccaatcaa
     5161 acggcaagac ttggtgaacc ttgtggccaa atacgcaccc cagacgggcg gactggcccc
     5221 gattccgggc gaaatgtctt cgaggccgtc tttgacgacg ggggccccac tgccgcagcc
     5281 cgcgagcaac tgaggaccga acacgcacgg tgcgtcaaaa ggtaggaaga atcaaacagt
     5341 aggaagacac gccggaccaa ggggttcgaa cgtcttgtac gtcaaaatga actcgcggtg
     5401 acctaaggcc tcggagactg tttgtttccc gccggcgact gcaatcatga attgccatat
     5461 tcgctcggcg acttcctcca gcgtgctttt gcccgtaaga acctccccgg cgttgatgtc
     5521 catgtcggat ttcatgcggg catatgtttc gggattggcg caaatcttga tcacgggtga
     5581 aatcgccgag ccaacaaccg atccccggcc cgtggtgaat aggatgagat ggcacccgca
     5641 ggcgattaac tccacgatct cggcgttgtc cgcgatattg gggaacccaa agcggacctc
     5701 accatcggga acaacatcta aaagatacaa cccaggggac ggcggaagat cacccggctt
     5761 gaggacacca gtgatcgggc gatgtccgct tttgaggtat gcccccagcg acttttcctc
     5821 gatggttgtt aaaccgccct ctgtgttgcc tggggcaaaa ctggcatgtc ccagggtagc
     5881 ataatatcgg gctgccttct ctacgcaagc acgtaacacg tcggccagtt ccggtcgagc
     5941 cgcccgttcg gctaagagcg attcacatcc cagcaattcg ccggtctctt cgaagatagc
     6001 cgtcgcaccg ctatcaacaa gtcgatcaaa gacgagacca cacgccggat tggccgtgag
     6061 tgaacttgtg gcatccgagc cgccacaaat ggtccccacg acgagatcac gtaactccat
     6121 gggacaccgc ggtaccttgg ccaactccgg gagcacactc ttgatccatt gccgtcccgc
     6181 cttaatcgtc cgtgccgtcc cgccaatttc ctgaattaca agaagctcca caggccgtcc
     6241 tgatgccgcg atctccgcac gaaggcgggc gcgatcgaaa ctttcgcatc cgagtgagac
     6301 caatagaacc gccccaacgt tgggatgggt tgtcacgcgg ctgaggacct tgtgggcgta
     6361 ttcacttggg tagcatccag gaaatccaat gagttccacg ccacgattag cgaacggcga
     6421 agcgatttcc gcagccacgt ggtgcgcgca ctccaccaga tagacgacga ccaagcaatt
     6481 gcggatgcct tttcgcccat cagcacggag ataaccttcc cactgagctg ccgtgcccga
     6541 tgtttgtccc tgggacgcct gcaactcttg tccaggagtt accgttgtca ttccgtacct
     6601 cctcgttttt gcgcgacgac gacaacttgc ggatatccta actgcgcgat tgctcgccct
     6661 ccccggcgat tattgaactg tggagtccga tcgctcgatt tcggccgggc tcgtcccctg
     6721 ttccagcttg ggctcattgg gctcgtttgg cgtcggggat ggcgtcgggc cttcctcaac
     6781 gaccggcgaa tcttgcgaag gcaaactgtc ggaagagaac gctttcgagt ttgcagaaat
     6841 ctcgccctcg gccgagggtc cagacagcgt ttccacaaca gccgacgtgc ccactgcgga
     6901 attctcgcca actggctcac ttactggctg cgtgcttacg tcagaggccg ggctggggcg
     6961 ttcgggtgtg ggaaggtagt cgctcgctaa attgtgtgtg tggacccatt cgccgggttc
     7021 aatattccgc gtggcatgtc cgatcggttg accaaacttg atgatctttt cgccctcggc
     7081 gataggacga gtggccacct tgcccgccat cggtacgttg gaaatgatct ggatcttcac
     7141 acggtccagc ttcaaaacgg tctccgcctt aatcggggcg gtggcaattg cgatgttatc
     7201 tcgcggatgt aaccgcaaaa gttttggctt catatcgccg atcctcaact ggccaaaaat
     7261 cctcaggtga cagctccgtg accctttccg caggcagatc cctgttgaaa aatctcccct
     7321 cagagagggc catttcctgc caaaaaagat ggccgacgtt caaatacata acctaccacg
     7381 gcagatgggc gaagaccaga ggcgaatcgc tatcgccggg ttctgcggcg gagttgctca
     7441 cccgtaaccg tattgttcca actcttcttc cgtcatgccg tagtaatgcc catattcgtg
     7501 gagaatcgtg atccgtatct gctttttgag acgcttctcg tccacataac cgtcacgacc
     7561 gacggcggac cacagtagcc cctcccggta aatggtcacg cgatctggta acgtaaccga
     7621 aggtgcgtcg aagtgcttct cgttgagcgg taccccggtg aataatcccc acagttctcg
     7681 ccgattgcgt atccccgttt ggcgcatcac ccttcgtgaa ggataatctt caacatagag
     7741 ggggactttg tccattgcgt ctcggacgag ctgtggcaac tcctcgatga cttcctcgag
     7801 gatgcggtcg aagtacctcc gagagtgttt atccatagcc cgagccaagt aattcaaccg
     7861 atgtggtttt actgcttttt cccgtgttcg ccgctgcaag tgtcctcgtg atcatcaaat
     7921 gccagtaatt caacacagac ccacgaccag gatcacgtga cttgcatcac cgcgcgtgat
     7981 tggcccataa ctgatgatcg aattcccacc ctttattttc gcaacctgtc aatcgggagc
     8041 cgagaatgtg ctcaagagcg aggttgcgcg taattatccg tttcttcggc ctgccttctc
     8101 gcggcgaggt tttgttacgt tcaagttcgc tgagggcgtc aagccggacg aaggtttttc
     8161 tttagaggcc gttttcgcac ggacatgggg attttgcttt ggccgtgttc cggcgtccga
     8221 ttggcaagtc gcggcgaacg aagtgtgggc aaaagttgcc aacattcccg tcggctggat
     8281 tcatgtttgg cctcgggaca cttccccgcc aggacggagc gaacccgaaa agccatggcc
     8341 agccgagatc agccaaattc aacaggtctt aatggagcac gcgccgacca aggcgattcc
     8401 ccccaatgtg tcgcaggaaa gtaccccatg cccgccgggc gtttgggtgc ttgattgtgt
     8461 ggtggtggaa ccaaccgagt ggtggattgg gattcaccgg gccgggagct tggaatcccg
     8521 ttatcctggg ggcaggttcc cgattgtttt accagaatac gccgtttcgc gggcatattt
     8581 gaagattgag gaagcgttgc ggtggagtgg gttcaagatt cccgccaatg cccgctgggc
     8641 agaattgggc agcgcccctg gcggggcgag ccaggcgctg ctcgatcgag gctacgaagt
     8701 tttgggggta gatcctgcgg aaatgcatcc gcttgtgtta tcccacccca aatttaccca
     8761 tctgcggcga cggacgcccc aagctccccg ccgggcgttt cgaaaggtcc gctggctggc
     8821 cgccgacatc aatgccgcgc ccaactacac tttggatgcc gtcgagagca ttgtgactta
     8881 tccccaggtc aacatccgcg ggatgatcct tacattgaag ttgccgaact ggcagctcgc
     8941 cgaaaacatt cccgagtaca tggagaggat tcgcagctgg ggttataaca tcgtaaaggc
     9001 ccgtcacttg tggaataatc gacaggaagt ctgcgtggcg gccctgatga agccgtttcg
     9061 tcggaagccc tcccactcgt gacccgcccg gaagttttgc acaatcgtta attctttgtg
     9121 atctgggaag gccacgtttg atttggagga ccgtgccgtt gtgggcatgc accttcatgc
     9181 aaaaaagaag tgagcaatac attgcttggc tttgcctgta tccgataagc ataaggacac
     9241 cttcgtgacg cgtcgtcatc gagcacagtc aaagtgttta ctgattgaga tgtcacggag
     9301 ggtttttgca agccgcgata ggaattcaac caagcctcta gccttcttcg gaggaaaacg
     9361 atggagtttg caaccccctg ttttcatgtg tcattttatg ttgcgatcct cgttggttgg
     9421 cttttgcctt cgattggagc gggcttagaa ccggggctgt acccttcgac gcgggactat
     9481 ccatttaccc gagctatggc tcctgattat cccaatttgc ctagtctgcc aacgccgggc
     9541 gacaactccc gctcggcgac tgtgacctcc tcgcggcttg aacaggccgg ggacgatgca
     9601 gaggatgcga ttatagaagg gccggacatg gcgctagaat cggctgattc acaatcggcc
     9661 ggcgcgatgg tccgagagga gggaaccaaa ccggacgatg agggtcttga gaaacaggac
     9721 gtcatggagg cacctcaatc gcccgatggg ccgagtgctg ctccccagaa accaaatgct
     9781 gatggtgatt catccacaga caagccgtca gcccgaagat cgactattga cccaacctcg
     9841 gtaggacgtc cgctagccgc gggaatcgcc aaactggtgg ctcccgagat cgagtctcaa
     9901 atggaaggcg caaacattac ggcccgattt cagcagtggc gctcttatgc ggcctcgcgt
     9961 ttggcggcaa cgcgggctga ctacacaagc tccgaaatca atggcatggc acgattgaag
    10021 tggtacgagc acctcttgct caatccgttg aatgtgcctc atgaaacaga ggaatttacg
    10081 cgactgcttc acgcgggttt aacggccaaa tcgagcgaag gtttccgctt tgccctccga
    10141 caagcacgat caaaaatgga tgtgcccgac gggagtttgc ctggtgacct cgaaacactg
    10201 tggaagtcgg aaaacaatga tccctacaag aagctggaga acatcttggt cacggcacga
    10261 aagacgtatg cacgaaccgt tgagcccctc acgcccgccg aactgaatga gttggcccag
    10321 cagcttaatt acgtgctgac cactaatacg attgtcggtc acaccgttga cagccgcagc
    10381 agcgccatga ggatggtccg tttactgcaa aaaatggatc gcagcgcgca atgggatttg
    10441 gctgaggtgt tggtcgccgt gtgcgacaag gagtttttag attcgttacg cacctctgct
    10501 ccgaaaggcg gggcgggggt tgcggtcgcc ggggtcacgg gtgaggtcct gagaaggatc
    10561 gacacacccg cggggaccat tctcgtggga ggaccaggaa aaaatgaata tcgcctggaa
    10621 gaaatgaaag atgtggcggc catcgtcgat gtgggcgggg acgacgttta tctggaaggc
    10681 acggtgtcgg tcgaacggcc gattctgatc atcattgatt tggcgggaaa cgatcgctac
    10741 gagggaaagc tgccgggtat tcaggggtct gcgatcattg gtggttcgat cctggtggat
    10801 tgtgcgggaa atgacatcta ttggggccag gatgttgccc aaggcagcgc gcttggcggt
    10861 gttggcatcc tcgttgatat ggccggggat gatcagtacc tagggtatcg tcgcgtccaa
    10921 gggcaggcca ttggcggtgt cgggattctc ctcgattacg gtggcaggga tgactatcgg
    10981 gcggcgatgt gggcacaggg gtttggtggt ccgctcggtt ttggcctgat cgacgatctc
    11041 gatggagacg accattatta tgtgggtggc cgattctacg actcctatcc cgaaacacca
    11101 ggttacgacg gctggggcca aggggttggc gccggcatcc gtggggcagc caatggtggg
    11161 attggcgtct tgttggaagg caatggcgac gacgtttatg aattcgacta ttttggtcag
    11221 gggggcgggt actggctcgc cattggcttg gcgcgagatt ttgccgggaa tgaccaacga
    11281 ctgggggcga cactcaatgc gtataatcga tccaatcgaa cgcagcaacg atttcagcgg
    11341 ttcggcaatg gttggggatg ccactatgct gtgggcgtct tgattgatga tctgggcaat
    11401 gactcgtacg acggttcaat catgggcacg ggcttcggct gggacctttc tgtgggttat
    11461 ctgctggaaa tgagcggtga tgaccagtac ctcgcccgtg tgggtggcgt gcagggacag
    11521 ggtgcgcaag ccagcctcgg ggtactctac gactttgagg gaaaagatgt ttaccgaggc
    11581 accagccagg ggtacgcgtc gtcatcgatc tcctatcacc cctatccaac ctgtggcggc
    11641 aattttagtt ttctcattga ttacggcggc gaggacgagt acggtagccg tgtccggaac
    11701 aacgcggtta gccggcgcgg aatgggcggt tttgtagtag atcggcccaa gcaggaagag
    11761 ttggacgcca tgcacgccag tgctaaaacc agcatctctt cgccgatcag caacagcagc
    11821 ggtcagtgag gccgatgtcg cttaatttgg gaaggaacag gtgtttttcc atcttacagc
    11881 ctatctcata ctcgcccctc tcccggaggg agagggggga cttattggga taggctctaa
    11941 ataaaaaggc ctgctggaag gaaatctcag caggcttctt ctgttttaga gatattgggg
    12001 caaagacaca cgtcgcgtta cggagccatc gcccccggtg caaaaccaac tccttgttgg
    12061 gcaaccgcct tgataacgag ctgggcgaga actttgagga cgccttcctg gaactcgatg
    12121 cgaactcgtg aaccgttctc gatcaacacg ccatccatag tgacacggtc tttggtgccc
    12181 gcagacgcga ggagttgctc ggcaagttgc cgctcttcct cgccccattc ttcgctggca
    12241 gcgacttgga cgaattcaat caatggcttg acagccaaag aaaactcgaa aatcttgtca
    12301 acggccacct cactggcgga ctgatccaag gcgtttttca gaatggacac gggattcggc
    12361 ccaaaagcca tacaaacaag gttttcgcta aagccaatga cggccccggc atttgagccg
    12421 aaaatgtcct taagggcctg cggtgcttcc ttcagcgcat cggcgggaag ctcgacgaca
    12481 tgcagccgaa cccccttgta catttccgcg ttgagtttca agactttggc aaaatcagga
    12541 ttgtctttcg cggcttgctt aagaatctcg gccacagtat tctccacttc ttctggattc
    12601 acaagcgtca tggccatgag tccttcacag cttttgctgt ctaatttgac cgacatggca
    12661 atatcgagct ggtctttttc gacggtttcg gccaaggact tgaaaaacgt cgaaaacagt
    12721 tgcttgagca gcttcttttc ccgaggcttt agcttcgtgt cttgatcaaa agcgctgctg
    12781 atcatacttt cgtactggga cagtgagtcc ttgaattgag tgatacttcg tggccctgct
    12841 ttgaccgtct cacggagggc agccgtggca tcgctcgcga tgaatccggc caatttcgtt
    12901 ttcaaatttt tgttaagggc cagttcttgc gccgattgcg tccccggaag gatcgtcgtt
    12961 tcgatatcga gcccgatcac gcttgccgct gcatcgatct ggacgccgat cagaatccgg
    13021 tccatctcat tgatggcctg ctgcatttgc tcaaacgaac gctggatcat ggccttttgg
    13081 gcggcgaatt gttcgtcagt ttgatcaggc tgttgctcca tcgccgtctg cacgccttgc
    13141 tgaatcatgg ccaaaccaag ttgcttccac atcggtggga tattgccgat atatgctgca
    13201 acgccgaggt catagatcga ggcgagctga tccagttcct ggggtggatt ggccgaggcc
    13261 tggtcaaggg cctctgggct cacgctgatc acggcccagt cacctttttg ggcgagaaac
    13321 aacgattggc cattgggcat atccactcga taggcagtcc cctgtttttc gggttcgact
    13381 ccacgtgtct ggagccaatc caacatcttt tgcaaatcga cgacaggcaa ataacctgcc
    13441 acgataaaat ctgcgccatt cgtttgcaca accaggccga ggggacgttt ggcatcgacc
    13501 ccgggcacgc ccggaacacc accggtttgc gccgcgagaa tctgtttcac gttatcgctc
    13561 agattactta ctcccgccag ttttcctaga aaatcggcat cttcccagag tcggtccaac
    13621 ccggagaagg acacaaccag cacgggcttt gtctgactcg gttgcgcgac gacctcgggc
    13681 aagcc
//
LOCUS       DAOSUT010000109        13667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2695_length_13667_cov_9.324640, whole genome shotgun sequence.
ACCESSION   DAOSUT010000109 DAOSUT010000000
VERSION     DAOSUT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2695_length_13667_cov_9.324640"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLD05_10600"
     CDS             complement(<1..374)
                     /locus_tag="PLD05_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77932.1"
                     /translation="MSQRRIAWSLIPVVLFLLGCQAKQGKETERAEPPGTPAAKVEAV
                     QPPEGVTQQETVPLSVTDENQQGPALADESSTTSGEAVPSQPPGEASLTAMQSTTGPY
                     WPRFHGPRGDNISDDTGLLESWP"
     gene            complement(462..1040)
                     /locus_tag="PLD05_10605"
     CDS             complement(462..1040)
                     /locus_tag="PLD05_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOP77933.1"
                     /translation="MKVLMLVGDYVEDYEVMVPFQALQMVGLTVHAVCPGKKAGEQVR
                     TAVHDFDGAQTYCELRGHNFTLNADFDQVRPEDYDALCIPGGRAPEYLRLNKRVIEIV
                     RHFGESGKPIAAICHGPQLLAAAGVLKGKICTCYPAVSPEVSNAGATYVQVELDQAYV
                     DGNLVTAPAWPAHPTWLARFLEVLGVSIRLPG"
     gene            1485..2498
                     /locus_tag="PLD05_10610"
     CDS             1485..2498
                     /locus_tag="PLD05_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOP77934.1"
                     /translation="MARPVTMFTGQWADLPLETLLQKVKEFGYDGVELACWGDHFEVQ
                     KALQDDEYCAKKRELLNKYGMQVFAISNHLVGQAVLDNIDSRHKAILPDYVWGDGDPA
                     GVNQRAADELKDTARAAQKLGVSIVNGFTGSSIWHLLYSFPPVPPDMIEEGYRLLAER
                     FIPILDVFAECGVKFALEVHPTEIAFDLYTAERTLEALKGHEAFGFNFDPSHLIWQGV
                     DPAQFIRYFPDRIFHVHMKDAIVKLDGKAGILASHLNFGDWRRGWDFRSLGHGSVNFE
                     EIIRALNDIGYEGPLSVEWEDSGMDREFGAKEACQFVRKINFAPSRVAFDAAFDKAEQ
                     KKA"
     gene            2784..3416
                     /locus_tag="PLD05_10615"
     CDS             2784..3416
                     /locus_tag="PLD05_10615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOP77935.1"
                     /translation="MLIYCIRHGESTYNSEGRVQGQSDVPLSDLGRRQGEATAEALAK
                     VGLEIIYSSPLRRAYETAEMVARRTGCDIRIDPRLMEIHAGIFQDRLRHELIREFPED
                     FRRWTSEDPDFVIPGGESRRQLAERGVAAFRDIAQSKFERVAVVSHGRLLVVTLKALL
                     VDSSHLIPPALQNASITVVEADPKTGQFRLVDVDQTDHLIGVGLSGRGDL"
     gene            3508..4971
                     /locus_tag="PLD05_10620"
     CDS             3508..4971
                     /locus_tag="PLD05_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP77936.1"
                     /translation="MLLMRCAPLSVCILAIAGALVMGTSAFAETNVPAKPNIIFILAD
                     DLGYGDLGCYGQKLIETPEIDRMAAEGMRFTQFYAGATVCAPSRCVLMVGQHTGHCSV
                     RGNAGANNRSIQSLREDDITISEILKKQGYATALIGKWGLGEVEEPGHPLRKGFDYFF
                     GYLNQGHAHNYYPEFLWRNWGKVMLRNEVVATKADYGSDFKPGYATKRVDYSHDLFAE
                     DALRWVEENRNSPFFLYLAFTIPHANNEGTRGTGDGAEVPDYGIYADRDWPNQDKGQA
                     AMITRMDRDVGRLLNKLRELGLAEKTLVLFSSDNGPHNEAGHNPKRFQPSGPLRGMKR
                     DLYEGGIRVPLIAWWPGTIQAGTVTDHIAYFGDLMATFCELTGADLPPGRDSISFLPT
                     LLGKSEEQKQHKYLYWEFYERGSAQAVRFGKWKAVRRPMLTGEIELYDLEADIGETTN
                     IAAQHPDIVAQARQFMEEAHVSDPRWQVPKPVVKPAG"
     gene            complement(5046..5936)
                     /locus_tag="PLD05_10625"
     CDS             complement(5046..5936)
                     /locus_tag="PLD05_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77937.1"
                     /translation="MSRILEAIQRLEEDERAEDRSVIPIFEGLAKNVAGSAPSPTGES
                     SPSDVSESDVRVFVRRFDSSHGETNNLEPPHFGKDDVQAATRATDDELEQRVMAICTP
                     YLKTIPAALKETWVLSAMWKTAATHAGWISSQVLDPHVPIECALALSSDVCEKIGQSV
                     LIIDGNPSSRLTRTLGLSPECDLIDVMLGRATLEESLIRLTRGGLYCLPIRQVGSAWV
                     LPNWADLNGLFQAVRQAFGFAVAFCPMSDDPLSLRILRNSDEFWLWCAAGSTPAGQIK
                     KVIQAASPKGDPAGAVLISL"
     gene            complement(6132..>6503)
                     /locus_tag="PLD05_10630"
     CDS             complement(6132..>6503)
                     /locus_tag="PLD05_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77938.1"
                     /translation="QGGEISGSGDPLSQKQTSTSLTVTGSAPTEELRSDSSEGDEFPT
                     DGNDHSDPETPPPSPIGKLTHPMPEPDPTGEPEAGTPVTLRFPMNIVHMEGGADKPQK
                     YRAIMKSLFTRLGKSGLEPIP"
     assembly_gap    6504..6603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6604..7751)
                     /locus_tag="PLD05_10635"
     CDS             complement(<6604..7751)
                     /locus_tag="PLD05_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP77939.1"
                     /translation="MYEQFFHFKERPFLPAPYPRAYVPVETMESAREQLRRAILRGEG
                     PALLVGPPGTGKTLLLQVLAEECGSRFRVVFLRHGRFPTSRALLQTILHELKQPFQGM
                     EDGELRIALMDFLKDEGGPAVVILADEADTLKLRLLEELRVLTNIQRSREPGLRLVLA
                     GSPVLEERLTSPKLQTLNQRIAVRCYLDALNRADTFTYIRTQISRAGAKAEQIFPENA
                     LQAVYHATGGVPRLINQICDHALVLAFADGLRQITAEVVEQAWADLQQIPLPWNPTRP
                     STPNTKGVIEFGSLQDETPDRDLFSVDQGNNGGAEGASRFGFLEKPESPPSVAPHLDD
                     VALSSSSKSDMEDGGHEIEAVSVENVMAVPNAGTSSSGEPALGDRPDPV"
     gene            complement(7770..9425)
                     /locus_tag="PLD05_10640"
     CDS             complement(7770..9425)
                     /locus_tag="PLD05_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77940.1"
                     /translation="MTRPKPKPWTFSARDAAAVSGFACGLLTAWLTLSLLASSDRPIF
                     VRSKPMSVTPAEAPHATLTLYYSPRLGPAPNPPGDGLQSDSRTPEGTDTDVARRLETL
                     VRELTTRLGSEASLEPIALPFHGGPGSDDGGVASLKATQEDHQAMDLDRTSSRRWPTA
                     RYLPLSITPGPGGSAGERTSNYVAGDYAGAWRIVFAKDTPPEQGMAKVRSALITLQRD
                     RAAAVADRVTRLAELKRQLQDLQTRFPQEMETLRHEWRERQTEFSRLAMSAIQNQVVR
                     GQLANDPLLAQLQATRESLLKRRQQLLMTHTVEHPDVKAVESLLAEVEQEIQLRSRRQ
                     KEYSANGVPGETNVPAATTWLSLPLGGQIGAPSAYEAPAIGNSHERPGLASNNQGEMQ
                     RLAALQSEIQSWDAKLGKGLDELRGNLEELQGQFVAVEAQLRDALTDWEVAQGCVVDF
                     DRESLSSVPREVTKTLTPFSLLFALLVGLLAHGAVTSLIIGFADGQRVSTAEQLFAIT
                     GGPVFHINLSTGDGKFIDGMDSVSPPHFATTQVESELSSKPRV"
     gene            9666..10337
                     /locus_tag="PLD05_10645"
     CDS             9666..10337
                     /locus_tag="PLD05_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOP77941.1"
                     /translation="MFRIKICGVRSRQDVVAVAEAGADAIGFNFFPRSVRYISPRDAS
                     QIGANVPRGIAKVGVFVDCPIGELREIAFRVGLHWIQLHGDETPDVLAKLRPLNCIKA
                     FRIDARGLEPVREFLVACEKLKVTPSAVLLDASENGEYGGTGQTFDWKIAAEYRNLMP
                     DVPPLVLSGGLTPENVAKAVETVRPAAVDVASGVEDEPGKKSPTKIKLFVAAAKAAYE
                     GIHAG"
     gene            10628..11950
                     /gene="ahcY"
                     /locus_tag="PLD05_10650"
     CDS             10628..11950
                     /gene="ahcY"
                     /locus_tag="PLD05_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOP77942.1"
                     /translation="MTRTKTEGKLPYKVADISLADWGRREIELAEKEMPGLMALRRKF
                     GASKPLRGARIAGCLHMTIETAVLIETLVELGAQVQWSSCNKFSTQDHAAAAIAARGI
                     PVYAWKGETDEEYEWCIEQTLIFPDGEQLNLIVDDGGDLTAMVHSPKYAHLLPGIRGL
                     SEETTTGVHRLYQMHARGELKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMV
                     AGKTAVVCGYGDVGKGCAQSLRAYGARVVVTEIDPICALQAAMEGYEVTTLEEVAPRA
                     QIFVTATGCCKVIRAEHMLAMPDGAIICNIGHFDVEIDVDWLMNKSGAKRTTIKPLVD
                     KFTFPNGKSIILLAEGRLVNLGCATGHPSFVMSNSFTNQVLAQIALWTEPEKYPVGVH
                     CLPKKLDEEVARLHLEQIGVKLTRLTPEQAAYLGVPVDGPFKPDYYRY"
     gene            12011..13399
                     /locus_tag="PLD05_10655"
     CDS             12011..13399
                     /locus_tag="PLD05_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOP77943.1"
                     /translation="MTKDNRSLLPKWGPVFLGLAGISEWDWIPMPEIQAFRGIRYDLG
                     HVGSLSDVIAPPYDVISPELQASLYKKHPCNVVRLILNRIEPGDDEVNNRYSRAKRFY
                     QSWLAEGVLFREADPAIYVYYQEFEVDGRRYLRRGFMARVRLSAYGEGQIFPHEHTLR
                     GPRMDQLMLMTMCKANLSQVFGMYPDPDQQTIAILDEAVQGLTPIEAVDHLGVIHRMW
                     PVTDPRLVTNLSAVVGPAPIFIADGHHRYETALEYREQLRDMGYLRPDHPAHYVLMHC
                     VPMEDPGLIVLPTHRLFRSIPPMTAAELAERLGDLFSLHQVANGPEAARDVWELVEAS
                     PDPATLAIFTRPDQQWSIAELTPAGREKMDDIATDHTPEWRALGVAVLHRLVVEHLLK
                     LEPPLDIDYVHSIEEVILGLQTSNYPMAVLVKPASVKDIRDVSLQGDRMPAKSTYFYP
                     KLCSGLVINPLE"
BASE COUNT         2951 a         3825 c         3860 g         2931 t
ORIGIN      
        1 ggccagcttt ctaggagccc tgtatcgtca gagatattgt caccccgtgg tccgtggaac
       61 cgcggccagt aaggcccggt tgtcgattgc atagcggtca aagaagcttc gccaggcggc
      121 tgggatggaa cggcctcgcc ggaggttgtc gaggattcgt ccgcaagcgc aggcccctgc
      181 tgattctcgt cagtcacaga taaaggaacg gtttcttgct gggtgactcc ttcaggaggt
      241 tgaaccgctt cgactttggc agccggagtc cctggcggtt cagcccgctc ggtctctttc
      301 ccttgtttgg cctgacagcc caatagaaac aggacgaccg gaatcaaact ccaagcaatc
      361 cgcctttgag acatcacgcg acccttctct ctaggtgtct gtcccgataa ggaacagata
      421 ctccaactgt gatcacgcca agacacgttt actggggtca atcatccggg caagcgaatg
      481 cttacaccga ggacctcaag aaagcgggca agccaggtgg gatgggctgg ccaggcgggg
      541 gcggtcacca aattgccatc cacgtaggct tgatccagtt ccacctgcac gtacgtggcc
      601 cctgcgttgg aaacttctgg cgagaccgcc ggataacagg tgcagatttt ccccttcagg
      661 acgccggccg ctgccaggag ttgggggccg tggcaaatgg ccgcgatcgg tttgcccgac
      721 tccccaaaat ggcggacaat ttcaatgaca cgcttgttga gccgcaaata ctcaggagcc
      781 cgaccaccag ggatgcataa agcatcgtaa tcctcggggc gaacttgatc gaaatcggca
      841 ttcagcgtga aattgtgacc gcgcagctcg cagtacgttt gggctccatc aaaatcgtgg
      901 acggcggtcc gaacctgctc accggccttc tttcccggac aaacggcatg caccgttagc
      961 ccgaccattt gcagggcctg gaacgggacc atgacttcat aatcctcaac gtaatcgccc
     1021 accaacatga gcactttcat gacggaaaac ctcctccaaa aacctttctt taaaaggtcc
     1081 agcaatcttc ccgagcaacc aaagctgtag caacgtattc tatcgaatcg cctttcccga
     1141 aaaagggttc catcgctcaa tacggctttt tcttggtagc aagccgggca aggaacgagt
     1201 cgtgtcaccg gggcaaaacc cgcccggtct ggagcgacga ggccatcttt aggttgctga
     1261 aaaaccctaa gggtggtgca acatcgggta gcggggcaat ctttaccgag gccatgagaa
     1321 cgcaatggac gaaaaaacgg gctcgcgatc atgtgggggt gttgaactcc ccacaaaata
     1381 tgcacaaaat acgaaccaag gtttgtgtca gtcgcttgct ggcggccgaa cggtttccaa
     1441 ttgtccagca tccgtggtcc ttttgaaatg ataaggagtc aatcatggcg cgaccggtga
     1501 caatgtttac gggtcagtgg gccgacttgc ccctcgaaac tctcctccaa aaggtgaaag
     1561 agttcggtta cgacggggtc gagctggcat gctggggtga tcactttgag gtgcagaagg
     1621 cgctgcaaga cgacgagtac tgcgcgaaaa aacgagagct gcttaataag tacggcatgc
     1681 aggtcttcgc cattagtaac cacctcgtgg gacaggccgt gctggacaac atcgattctc
     1741 gtcataaggc cattctgccc gattacgtct ggggtgacgg cgacccggca ggtgtgaatc
     1801 aacgggctgc ggacgaactg aaagacacgg ctcgcgccgc ccaaaagctc ggcgtcagca
     1861 ttgttaatgg gtttacggga tcgagtattt ggcatctcct gtattcattc ccgcccgtcc
     1921 cgccggacat gatcgaggaa ggctaccgcc tgttggccga acggttcatc cccatcttgg
     1981 atgtgtttgc cgaatgcggt gtgaaattcg ctctagaagt gcatcccacg gaaatcgcct
     2041 tcgacctgta caccgctgaa aggacgctag aggctctgaa aggtcacgag gctttcgggt
     2101 tcaacttcga tccgagccac ttgatctggc agggtgttga ccccgctcaa tttatccgct
     2161 acttcccgga tcgtatcttc cacgtccata tgaaagacgc catcgtcaaa ctcgatggga
     2221 aggctggcat cctggcaagc catctcaatt ttggtgattg gcgacggggc tgggacttcc
     2281 ggtcgctcgg ccacggtagc gtcaacttcg aggagattat ccgagcattg aacgacatcg
     2341 gttacgaagg gccactctct gtggaatggg aggacagcgg catggaccgc gaatttggcg
     2401 ccaaagaagc ctgccagttc gtgcggaaga tcaatttcgc tccgagccgg gtagcattcg
     2461 atgctgcctt tgacaaggca gaacaaaaaa aggcgtgatc ggataagcgc ttcgactgtg
     2521 tcgtgtggaa aaaaaggatt attgagggtt cttaaagtct atcccacaac ctcccctctc
     2581 ccgttcagcg ggagaggggt cgggggtggg ggtggttcga atcgctgccg gttttgtcgc
     2641 tggactccga cggtcgtttg ccctcacccc aaccctgtcc cagagggaga gggaattttg
     2701 ggataggttc ttaattgaca cggtcggttg ccggccgtgc caattttatg cgcagacgct
     2761 tcttgccgta atccgttcaa accatgctga tctactgtat tcgtcacgga gaaagtacgt
     2821 acaactccga gggacgtgtt caaggccaat cggatgtccc cctctctgac ttaggacgcc
     2881 gccaaggcga ggcgacagca gaggccctgg caaaagtcgg gctggagatc atctattcca
     2941 gtcccctgcg acgggcttac gaaacggcag agatggtggc ccggcgtacg ggctgcgaca
     3001 tccggattga ccctcgtctg atggagattc atgcgggaat cttccaagat cgcctacggc
     3061 atgaactgat ccgggagttc cccgaggact tccgacgctg gacctccgaa gaccccgatt
     3121 tcgtcatccc cggtggcgag tcgcgacgac agcttgcgga acgcggcgtg gcagcgtttc
     3181 gggacatcgc ccagtcgaaa tttgagcgtg tggctgtcgt ttcgcacggg cgcctccttg
     3241 ttgtgacgtt aaaggcactt ttggtagata gttctcactt aatccccccg gcgctgcaaa
     3301 atgcgtcgat taccgtcgtc gaggcagatc ccaaaacggg tcaattccgc ctggtggatg
     3361 tggatcagac cgaccacctc attggcgtcg ggctttcagg tcgcggcgac ttatgacaag
     3421 ggttgcgata ccttctccaa aagaacaaaa tatttgctgg taaccttgtt tttggtctcc
     3481 acaaccatcc atcggggagg aacaggtatg ttgctgatgc gctgtgctcc tttatccgtg
     3541 tgcattctcg ccatcgcggg tgcccttgta atgggaacct ccgcattcgc cgaaaccaac
     3601 gtgccggcca aacctaatat cattttcatc ttggcagacg acctgggtta cggcgatctt
     3661 ggctgttacg ggcagaagtt gattgaaaca ccagaaattg atcgcatggc tgctgaaggg
     3721 atgcgattta cccagttcta cgccggggcc accgtatgtg cgccgtcgcg gtgtgttctc
     3781 atggtcggac agcacaccgg tcactgttcc gtccgcggca atgccggagc gaacaaccgc
     3841 tcgattcaat cgctgcgcga ggatgacatc acgattagtg aaatcctgaa aaagcaagga
     3901 tacgcaacgg ccctcattgg aaaatgggga ttgggcgaag tcgaagaacc tgggcatcct
     3961 ctccgcaagg gtttcgatta cttctttgga tatctcaacc agggacacgc gcataattat
     4021 tatcccgagt ttttgtggcg gaactggggt aaggtcatgc ttcgcaatga ggtagtggcc
     4081 accaaggccg attacggctc ggacttcaaa cccggttacg ccaccaagcg ggtcgattat
     4141 tctcatgacc tgtttgcaga agacgccctt cgctgggtgg aagaaaaccg gaactctccc
     4201 ttcttcctct atctcgcctt tacaattccc catgccaaca acgaggggac acggggaacc
     4261 ggcgatgggg ccgaagtgcc cgattacggc atctatgccg atcgagattg gcccaatcaa
     4321 gacaaaggcc aggcggccat gatcacccgc atggaccgcg acgttgggcg actcctcaat
     4381 aagttacggg agttgggact ggccgagaaa acactggtct tgttttcttc ggacaacggt
     4441 ccccacaatg aggcgggcca caatcccaag cgattccaac cgtcggggcc acttcgcggg
     4501 atgaagcgcg acctttacga gggtggtatc cgcgtcccct tgatcgcctg gtggcccggg
     4561 acaattcagg cgggcacagt cactgaccat atcgcttatt ttggcgatct gatggccacc
     4621 ttctgtgaac tcaccggggc tgaccttcct cctgggcgag attccattag ctttctgccc
     4681 actttgttag gcaaatccga ggaacagaag caacacaagt atctatattg ggagttttac
     4741 gaacgggggt cggcacaggc cgtgcgcttt ggaaaatgga aggccgtccg ccgacccatg
     4801 ctgacgggtg agattgaact gtacgacttg gaggccgaca ttggggaaac gaccaacatc
     4861 gctgcccagc atcccgacat cgtcgcccag gcacgtcagt tcatggaaga agcccatgtg
     4921 agcgatcccc ggtggcaggt gccgaagccc gtcgttaagc ccgccggata ataccgagct
     4981 gcgttccagc cgaaacgctt taaaccgttc tcccaaaccg atagggaaag cgtgctgcgt
     5041 ggccttcaca gcgaaattaa gaccgccccg gcgggatcgc cttttggact cgcggcttgg
     5101 atgacctttt tgatttgacc tgccggcgtg gatcccgctg cacaccacag ccagaattcg
     5161 tcgctattac gaagaatccg caatgacaat ggatcatcgc tcatagggca gaacgcaaca
     5221 gcgaatccaa aagcctgacg caccgcctgg aagaggccat tcaaatccgc ccagttgggc
     5281 aacacccagg ctgatcccac ctgccggata ggtaggcagt aaagcccacc gcgggtgagt
     5341 cggatgaggc tttcctccaa agtggctcgg ccaagcatca cgtcgatcaa atcgcattcc
     5401 ggcgacaatc caagagttcg cgttaaccgc gaggacgggt taccgtcaat gatgagcacg
     5461 gactggccga tcttctcgca aacatccgac gaaagcgcca atgcgcactc gatggggaca
     5521 tgcggatcga gaacttgact actgatccag ccggcatggg tggcagcggt cttccacatg
     5581 gccgagagca cccacgtctc tttaagcgcg gcagggatcg tctttaaata tggtgtacaa
     5641 atagccatga cgcgctgctc aagctcgtca tcagttgccc gcgtcgcagc ctgaacgtcg
     5701 tctttaccaa aatgcggcgg ctcaagatta ttggtctcgc cgtggcttga gtcaaaacga
     5761 cgtacaaaca cgcgcacgtc ggattccgac acatcggatg gagaagactc accggtaggg
     5821 gacggcgcgg agcctgccac gttttttgcc aggccctcaa agatcgggat gacggacctg
     5881 tcttccgctc tttcgtcctc ctcgagacgc tggatcgctt ccaaaattcg actcatcttt
     5941 ttgattggct cctaattcat tcacgccaac acgggcactg ccagttagag cctatcccaa
     6001 aaagtccccc tctccctccg ggggagggtt ggggtgaggg caagcgaaca tcagaatcga
     6061 ttgagaaaac cggcagcgat tcgagtcacc ctcacccccg acccctcgcc cgccaaatgg
     6121 gcgaggggag gttatgggat aggctctaag ccgctctttc ccagccgcgt aaataatgac
     6181 ttcatgattg cgcggtactt ctggggctta tcggcaccgc cctccatgtg cacgatattc
     6241 atcggaaaac gcagcgtcac tggtgtcccc gcttctggtt cacctgtcgg atcgggttcc
     6301 ggcattggat gcgtcagctt cccaattggc gagggaggtg gcgtttcggg atcggaatgg
     6361 tcgttcccat ccgtcggaaa ttcatccccc tcagagctat cactccgcag ctcttcagtc
     6421 ggtgcagagc cggtgacggt gagtgaagtc gatgtttgct tttgtgaaag cgggtcaccg
     6481 ctacctgaaa tttctcctcc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnaccggat caggccgatc tcctaatgca ggctcccctg atgagctggt ccccgcattt
     6661 gggactgcca taacgttttc gaccgatacc gcttcaatct cgtggcctcc atcttccatg
     6721 tcacttttag agcttgacga aagggccaca tcgtcgaggt gtggcgccac gctaggcggt
     6781 gactcgggct tttccaaaaa tccaaagcga gaggcgcctt ctgccccacc gttgtttccc
     6841 tgatcgaccg aaaacaggtc tcgatctggc gtttcatcct gtaatgaacc gaattcgatg
     6901 acccctttgg tgtttggtgt cgaaggtcgt gtgggattcc agggcagagg gatctgctgt
     6961 aagtctgccc atgcctgctc aacgacctcc gcggtaatct gccgaaggcc atcggcaaat
     7021 gccaggacca aggcgtgatc acaaatttgg ttgatcaatc gcggcacgcc gcccgtggcg
     7081 tgatacaccg cttgcaaagc gttttccgga aagatttgct cggctttagc accagcccga
     7141 ctgatttgag tgcgaatgta ggtaaacgtg tcggcccggt tgagggcgtc cagataacag
     7201 cggacggcaa ttcgttggtt caacgtctgt agtttcggcg aggtcaacct ttcttccaag
     7261 accggtgagc cggcgagaac cagccgcagc cctggttcac ggctccgctg gatgtttgtc
     7321 agaacgcgca gttcttccaa aaggcggagc ttgagggtgt ctgcctcatc tgccaggatc
     7381 accaccgctg ggccaccttc atctttaagg aagtccataa gggcaatccg caactcgcca
     7441 tcttccatgc cctgaaaggg ctgtttcagt tcgtgaagaa tggtctggag gagggcacgg
     7501 gaagtgggaa accgcccatg acgcaaaaag accacacgaa aacgggaacc gcattcctcc
     7561 gccaggacct gcagtaataa tgtcttgcct gtgcccggtg gaccaactaa gagcgccgga
     7621 ccttcacccc gcaaaatcgc tcggcgaagt tgctcacggg cagattccat tgtttccacg
     7681 ggcacgtaag cccgcgggta aggtgccggc aagaacggtc tctccttgaa atgaaaaaac
     7741 tgctcgtaca tcgacccatc cgtgtagatt tagactctcg gttttgagct cagttctgac
     7801 tccacctgtg tcgtcgcaaa atggggtgga gataccgaat ccatgccatc gataaatttc
     7861 ccatccccgg tggacaggtt tatgtgaaaa accggtcccc ccgtgatggc aaataattgc
     7921 tcagcagtag aaacacgctg gccgtcggcg aaaccaatta ttagtgacgt gacggcccca
     7981 tgcgccaata gtcccaccaa aagggcaaac aacagcgaaa acggcgtcag agtctttgtc
     8041 acttccctgg ggaccgagga tagggattct ctgtcgaaat cgacgacgca tccttgggca
     8101 acctcccagt cggtcaaggc gtctcggagt tgagcctcaa ctgcgacgaa ctgtccctgc
     8161 aattcctcaa gattgccgcg aagctcgtca aggccctttc ccagctttgc gtcccacgat
     8221 tgaatctccg attgcagagc cgcaagacgc tgcatctccc cctggttgtt tgacgctaat
     8281 ccaggccgct catgtgagtt ccctatcgcg ggcgcctcat aggcagacgg agcaccaatt
     8341 tgtccaccaa gcgggaggga gagccaggtc gtggccgcgg ggacgtttgt ttctcccgga
     8401 acaccgtttg ccgagtattc cttctgtcgc cgagatcgta attggatctc ttgttcaacc
     8461 tcggccagaa gcgattccac cgctttaacg tccggatgct cgacggtgtg ggtcatcagt
     8521 agctgttgac gacgcttgag aaggctctca cgtgttgctt gcaattgggc gagcagggga
     8581 tcgttggcca gctgaccgcg cacgacctgg ttttgaatcg ccgacatcgc cagccgagaa
     8641 aactccgtct gcctttctcg ccactcgtga cgtaacgttt ccatttcctg tggaaaccgt
     8701 gtttgcaagt cctgcaattg acgtttaagc tctgccagcc gtgtaacacg atcggcaacg
     8761 gcggcggctc ggtctctttg cagtgtgata agagcagaac ggactttcgc catgccctgc
     8821 tccggaggcg tgtccttcgc aaatacgatt ctccatgccc ccgcgtaatc ccctgcgaca
     8881 tagttgctgg ttcgttcgcc agcggagcca cctggtccag gtgtgatgct cagcggcaga
     8941 taccgggcgg tgggccaacg ccgtgaagac gttctatcta agtccattgc ctggtggtct
     9001 tcctgagtcg ctttcaaaga ggcaacaccg ccgtcatcag aaccagggcc gccgtgaaaa
     9061 ggcaatgcga ttggttcaag actggcttcc gaacccaagc gggtcgtcaa ctcacgaacg
     9121 agtgtttcca gtcgccgggc aacatccgta tccgtcccct cgggggtccg cgaatcgctc
     9181 tgtaaaccat ctcccggagg gtttggcgca ggccccagcc gtggtgaata atagagtgtt
     9241 aacgtggcgt ggggtgcctc ggctggagtg actgacatcg gctttgagcg gacgaagatt
     9301 ggcctgtcgc tcgatgcgag aaggctaagg gtgagccagg ccgtgagcag tccgcaggca
     9361 aaaccgctca ctgcggcggc gtctctggca gaaaaggtcc aaggttttgg cttgggtcgt
     9421 gtcaagagac acctccccaa tttgttcgtt cggccatcaa ggagacggtt gacctcggtc
     9481 aatgcctctg gtgaaaggta tccccaacgt tgagtcggca atccgagccc tccccgcacg
     9541 gagcgggggg cttcctgagt gaaaatcgac tttgtatggt ctaagcttga gaaggccttt
     9601 gcgggtcgta acggtttggc ctggtttttg ggtttacctc aaatcgcagt atgtgaagag
     9661 gtccaatgtt tcggataaag atttgcgggg tgcggtctcg acaggatgtg gtcgcggtgg
     9721 ccgaagccgg agctgacgcg attggcttta acttttttcc ccgaagtgtg cggtacatct
     9781 cgccccgtga tgccagtcaa ataggcgcaa acgtcccccg gggaatcgcc aaggtggggg
     9841 ttttcgtgga ttgtcccatc ggggaactcc gtgaaatcgc ctttcgtgtc ggtttacact
     9901 ggattcagct ccatggggac gagaccccgg acgtcctcgc aaagctccgc cccctcaatt
     9961 gcatcaaggc ctttcggatc gacgcccgcg ggctagaacc tgtccgggaa tttctcgtag
    10021 cttgcgaaaa actgaaggtg acccccagcg cggtgctttt ggatgcatcc gagaatgggg
    10081 agtacggcgg cacaggacag acgtttgact ggaagatcgc agcagagtac cgcaatctca
    10141 tgccagacgt cccgcctctc gtcctttcgg ggggacttac cccagaaaac gtggccaagg
    10201 ccgtcgagac cgtccgccct gcagccgttg atgtggcgag cggggtggaa gacgagccgg
    10261 gcaaaaaatc cccaacaaaa attaaactct tcgtcgctgc ggccaaggcg gcctatgagg
    10321 ggatccacgc aggttaggta cactttcgcc gacagttagc agcagaaggc cctaaccgtg
    10381 gcacgtcaag ggacttggca ggggcaggaa actcgtcgta tgatagtggg gtaactgtaa
    10441 actgtgccaa tttcgtgcct tacctttttc aaccagacgg gctgtccgcc acgttgagga
    10501 cttgggtact tcatccggaa gaaaactccg gaggctccgg ccgtcggtag agtctcgtga
    10561 gaagggcgag gttttcacag cgatcattcc cagtgaggca ggagagtaac aaatgtccca
    10621 cacagcgatg acccgaacca aaaccgaagg caaactccct tacaaggtgg ccgatatttc
    10681 cctggccgac tggggacggc gagaaattga attggccgag aaggaaatgc caggcttgat
    10741 ggccctacgc cgtaagtttg gcgcgtccaa gccgttgcgg ggggcgagga ttgccgggtg
    10801 ccttcacatg acgatcgaaa ccgccgtctt gatcgaaaca cttgtggagt tgggtgccca
    10861 ggtccaatgg agcagttgca acaagttttc aactcaagac cacgcagcgg cggccattgc
    10921 agcccggggt attcccgtct atgcctggaa aggcgaaacc gacgaggagt acgaatggtg
    10981 tatcgagcaa acgctcattt tccccgacgg ggagcagctc aacctgatcg tggatgacgg
    11041 cggggacctc accgccatgg tccacagtcc caaatatgcc catttgctgc cgggaattcg
    11101 tggcctttcg gaagagacca ctacaggcgt gcatcgcttg tatcagatgc atgcccgcgg
    11161 cgagctaaaa gtgcccgcca tcaacgtcaa tgactcggta acaaagagca agttcgacaa
    11221 cctctatggt tgccgcgagt cactgatcga cggcatcaag cgagccacgg acgtcatggt
    11281 agccgggaag accgccgtag tctgcggcta tggcgacgtc ggaaagggtt gtgcgcaatc
    11341 gctgcgggca tacggggcca gggtggtcgt taccgaaatc gaccccattt gtgcccttca
    11401 agccgccatg gaagggtacg aggtcaccac gctcgaagaa gtggccccgc gagcgcagat
    11461 tttcgtcacg gccaccgggt gctgcaaggt gattcgggcc gaacacatgc tcgccatgcc
    11521 ggacggtgcc atcatctgca atattggcca ctttgatgtg gaaatcgacg ttgattggct
    11581 gatgaacaag tccggggcca aacgaacgac gatcaaaccc ctcgtcgata agttcacgtt
    11641 ccccaatggt aagtcgatca tcttgttggc cgaaggccgc cttgtgaatc tgggttgcgc
    11701 gaccgggcat ccgtcgttcg tcatgtcaaa ttccttcacg aaccaggtcc ttgcccagat
    11761 cgccctctgg actgagccgg agaaataccc cgttggcgtc cattgcttgc ccaagaagct
    11821 cgacgaggaa gtggcccggt tgcatctcga acaaatcggc gtcaaattga cgcgactgac
    11881 accggaacag gctgcctatt tgggcgttcc tgtggacggt ccgttcaaac ctgactacta
    11941 ccgttactaa ttgtggccgc gacaatcggc gacacggagc catcttgtcg gtgggccgac
    12001 cataggacaa atgacgaagg acaatcggtc ccttttgcca aaatggggac cggttttttt
    12061 gggacttgcg ggaatcagtg agtgggattg gattccgatg ccggagatac aagcttttcg
    12121 agggattcgc tatgatttgg gtcacgttgg ctcattaagc gacgtgatcg caccgcccta
    12181 tgacgtcatc tcgcctgaac tccaggcctc cctctacaaa aagcacccat gtaacgtggt
    12241 gcggctcatc ctcaaccgca tcgaaccggg cgacgatgag gtcaacaacc ggtattcgcg
    12301 ggcaaaacgg ttttaccaga gttggttggc ggaaggggtg ctctttcggg aggccgatcc
    12361 cgcgatctac gtctattacc aggagtttga ggtagatggc cgccgttatc tccgtcgcgg
    12421 gttcatggcc cgagtgcgtc tatccgcgta cggcgaaggg caaatcttcc ctcatgaaca
    12481 tacactccgt ggtccccgca tggatcagct tatgctaatg accatgtgca aggccaactt
    12541 gagtcaggtc ttcgggatgt atcccgaccc cgatcaacaa acgatcgcca tcctcgacga
    12601 agccgttcag ggcctgaccc ccatagaggc cgtggaccac ctgggcgtga tccaccgcat
    12661 gtggcctgtc actgatcctc ggcttgtgac gaatctgtcg gcggttgttg gtccagctcc
    12721 gattttcatt gccgatggcc accaccgtta cgagacggcc ctggaatatc gggaacaact
    12781 ccgggacatg ggctatcttc ggccggacca tcctgcccat tatgtcctga tgcactgtgt
    12841 ccccatggag gatcctgggt tgatcgtcct gccaacgcat cggcttttcc gctctatccc
    12901 gcccatgacg gcggccgaac tggcggaacg gctcggcgac ctattttcgc tccaccaagt
    12961 ggcaaatggt ccggaggccg cgcgggatgt ctgggagttg gtagaggctt cccccgaccc
    13021 cgcaaccctg gcgatcttta ctcgtcccga tcaacagtgg tccatcgcgg agctgacgcc
    13081 cgccgggcga gagaagatgg acgacatcgc caccgaccac acaccggagt ggcgtgcttt
    13141 gggcgtagcc gttctgcatc ggctcgtcgt cgagcacctc ctcaagctgg agcccccctt
    13201 ggacatcgac tacgttcaca gcattgagga ggtcattctg ggcttgcaaa caagtaacta
    13261 tccgatggcc gtcctggtca agccggcttc tgtgaaggac attcgcgacg ttagcctaca
    13321 aggagaccgg atgccggcca agagcaccta cttttatccc aaactgtgct cagggctggt
    13381 aattaacccg cttgagtaag ccggcaattg gtctcttcag agggcgtgat aagtgaggca
    13441 aaagcccaac gccggtcctg gttttcgctc cgcctgagtg aatttcccgg tttcacgtgg
    13501 aacaggatct aattcctcgg tagcttggag cctatcccat aagctccctt cacgcgtttg
    13561 gtgggcgagg ggtcgggggt gagggtggct cgaatcgctg gcggttttct cgatcgattc
    13621 cgacgttcgc ttgccctcac cctaaccctc tccgggaggg agagggg
//
LOCUS       DAOSUT010000110        13663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2696_length_13663_cov_9.488830, whole genome shotgun sequence.
ACCESSION   DAOSUT010000110 DAOSUT010000000
VERSION     DAOSUT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_13663_cov_9.488830"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1348)
                     /locus_tag="PLD05_10660"
     CDS             complement(188..1348)
                     /locus_tag="PLD05_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOP77944.1"
                     /translation="MSTRSPQKIAIRFIASLKMVGAILVTLALSSSGWAIDVVKFRGD
                     NGITEATGKILVQAADGGVLLLTPVGVLLPLGSSEVIEITQNDLPFQAATPKEAATYW
                     LQRLPKNFEVYTTAHYVVLHNASRDYARWCGSLLERLYNGFTAYFSHKGLTIERPAFP
                     LVIVVFATRQQYIEHCREEVGDAIERIQGHYNLETNVVTTYDLTGSNGTATAAQIARY
                     IMQPGVQQTITTLVHEASHQLANNSGVTCRWTDTPQWVSEGLAMFFETPDLRSSRGWS
                     TVGALNYERLQRFRAYLPQRPSDSLATLIRDNSRFQNLSTAMDAYAEAWALTYFLLQQ
                     NGQAFITYLARLREKSPLVYDSPEERVEDFSQAFGDLRQVESEFLRYILRLR"
     gene            complement(1423..2832)
                     /locus_tag="PLD05_10665"
     CDS             complement(1423..2832)
                     /locus_tag="PLD05_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOP77945.1"
                     /translation="MGVRLEVIQFFDETNRSLIQRIPAEGSADIKLGAQLIVYENQEA
                     VFFRDGKALDRFGPGRYTLTTANVPLLTRLFTWPWEKSPFQCSVYFVGKQTFLDQKWG
                     TRQPIVFRDAQFGMIRLRSFGKYSFRVADSSVLINTLVGTQGKYTTEEVTNFLKDLIV
                     ARLTDFLGTLNISVLDLPAKYDEVSAGTRAKVAADFAKYGLEIVDFFINAITPPEEVQ
                     RMIDARSGMGAVGDLDAFLKYQIAQSLTKLSESGGESAGAMGMGYGAGFGMMLPQMIR
                     EAMRETAGPPPLPTTGGQAPLQTGPAGPAPQAVPPAVPLAEKAGQQAAPPPLGFPSEG
                     ASDPLQLVKTVARQSGYVLEEFGNTLKITVPVGNLRKQVVWCSYEQSREGGQNIVRFW
                     SVCGPASEQNAAMLLRHNAQLTFGAFAFHKVNNQELIVIQMSLPANSLTIVDVARFIP
                     ALAWQADRFEESLSDQDIF"
     gene            complement(2911..3420)
                     /locus_tag="PLD05_10670"
     CDS             complement(2911..3420)
                     /locus_tag="PLD05_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77946.1"
                     /translation="MVEPMDHTKKRNWVETILRHIPGFRGYLEKEYRRDSDNLQREWL
                     AHRLELAKCSVDNLMGLLAEGGQVDQLATLEQVRGRIDHIVARIRGAMEGYSGFFDLV
                     QIDEEVLDRVYQFDLSLAERVEQLAEAVERLPRCPTDLSCAIGEVRQSLDELHRLWDM
                     RHELLAGLR"
     gene            3961..4620
                     /locus_tag="PLD05_10675"
     CDS             3961..4620
                     /locus_tag="PLD05_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP77947.1"
                     /translation="MSLLAYLAVGACLCGCRTGFLSVQKKARQELPPGIVLPREKIEK
                     WRELVNSAKDSGSAATATARLQEAFQGEDDPQLRAELVRLAGSLPPEAAWPIVELGLR
                     DENPDVQVQACRMVAKAKMAQAVGSLAKLAEESSNQDVRQAAIKALGQFKDPVAIQAL
                     AKALRDRDPAIQYLAIHSLRTVTGQDFGYDVAQWLAFLEGRQNPPQPTRAMATRPTQR
                     R"
     gene            5118..6407
                     /gene="eno"
                     /locus_tag="PLD05_10680"
     CDS             5118..6407
                     /gene="eno"
                     /locus_tag="PLD05_10680"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOP77948.1"
                     /translation="MSSTIVDILGRQILDSRGNPTVEVEVLLEDGSLGRAAVPSGAST
                     GTREALELRDGDPSKYLGKGVSQAVQNVNEKIAEEICGMDALDQAAIDYAMIELDGTE
                     NKSNLGANAILGVSLAVAHAAAQSSGLPLYRYLGGALARVVPAPMMNILNGGAHADND
                     VDIQEFMVMPLGFETFSDAIRCGCEIFHNLRKVLKDRKLSTNVGDEGGFAPDLKSNEE
                     ALECIKEAIDRAGYQLGEQVFLALDLAASELAVEGKKGMYMIGGKEVPAKAVIDMLES
                     WLEKYSICSIEDACSEDDWDGWALLTERLGKKVQIVGDDVFVTNTKIFAKGIESGIAN
                     SILIKVNQIGTLTETFECIDMARRAGYTYVISHRSGETEDTTIADIAVAAGGGQIKTG
                     SASRTDRIAKYNQLLRIEEMLGSTAIYGGKLFPKQWK"
     gene            6649..8199
                     /gene="guaA"
                     /locus_tag="PLD05_10685"
     CDS             6649..8199
                     /gene="guaA"
                     /locus_tag="PLD05_10685"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOP77949.1"
                     /translation="MTNPTEEKILVLDFGSQYAQLIARRVREQNVFAVIVRHDISAER
                     VQELAPKGLIFSGGPASVHWSDSPKCDPDLFQLDIPILGICYGMQLVCQSMGGKVESD
                     RGREYGPAICRVIQQNELFTDLPSEFQVWMSHGDQVTSISEEFVVLAATESCPLAAVR
                     HRSRPLYGLQFHPEVTHTTYGSQILRNFVHKVCQCSGTWKLSDFAEETIKEIRQRVGR
                     DRVICGLSGGVDSSVTAALLSRAVGDQTACILVDNGLLREGEVESVIEEFTKHFQTDL
                     HVVRAEDRFLAALRGVVNPQEKRKIIGRVFVDCFAEEAAKIPNARFLAQGTLYPDVIE
                     SGGLRDGPAATIKLHHNVGGLPEDLQFELIEPLRNLFKDEVRNLGMQLGLPDEIVWRH
                     PFPGPGLAVRCIGEVTKERLNILRQADAIVIEEIKRAGWYRKTSQAFAVLLPVQTVGV
                     MGDARTYEEVIAVRAVDTEDFMTADWSKLPYELLARISTRIINEVSGINRVVYDISSK
                     PPATIEWE"
     gene            8294..8650
                     /gene="xseB"
                     /locus_tag="PLD05_10690"
     CDS             8294..8650
                     /gene="xseB"
                     /locus_tag="PLD05_10690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOP77950.1"
                     /translation="MNVSESDRCPSGEESTPRFEDALRELEAIVERLERGQLGLDEAL
                     AEYERGVRLIRHCRELLQKAERRIEILSGIDEQQRPITQPFEEEDLSLEEKREKRSRR
                     RGVKNLSDEESESEAG"
     gene            8741..9640
                     /locus_tag="PLD05_10695"
     CDS             8741..9640
                     /locus_tag="PLD05_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOP77951.1"
                     /translation="MDFALNWQETEKHLAELRDLVNQWLDKLTDLPDGCPDRLKAAMR
                     YSLLAPGKRLRPTLVLLAAEACGADPEEAIAPACAIEMIHCYSLIHDDLPAMDNDDLR
                     RGQPTCHRQFDEATAILAGDALLTLAFETLSRYMEPADRAVQCCRLLAEAAGPCQLVG
                     GQADDIQSPTLTPTLDLLERIHRRKTGALIECALKMGGIVGSANSEVLNGLSEYGRAI
                     GLAFQITDDLLDLLGDEKLVGKRLQKDNKQKKLTFPSLLGVEESRKRLDELIEAACRV
                     LEGLPIERRHLVALAINLKSRTH"
     gene            9640..>10467
                     /locus_tag="PLD05_10700"
     CDS             9640..>10467
                     /locus_tag="PLD05_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOP77952.1"
                     /translation="MDKLLSKIQSPHDLQSLTSAQLEQLAAEMREAMCQLLGTRSAHF
                     ASNLGVVELTIALHTTFDFSRDRLIWDTGHQVYPHKMVTGRYRQFHTIRTKGGLMGYP
                     NPAESPYDLFMTGHAGCSISTAIGLKCGDDLLRPDENRYVVAVIGDGAFPSGIVSEAM
                     NHAGWLKKRLIVILNDNRMSICQRVGGIAEYLDRLRMAPLYSGIKNEVYNLLSKVPLV
                     GNSMERLLDQLRDAIKAGMLGGMLFEELGFRYLGPVDGHNIGQLQKYLRMVKQFPGPV
                     "
     assembly_gap    10468..10567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10568..11407
                     /locus_tag="PLD05_10705"
     CDS             <10568..11407
                     /locus_tag="PLD05_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOP77953.1"
                     /translation="GICESHAVGFAAGLAKAGLRPIVDIYSTFLQRAYDQLFQEAALQ
                     NLPIVFMMDRAGVVGADGPTHHGVFDIAYFRHFPNMTVLAPGDADELEAMLAVALQQE
                     GPVAIRYPKATATVVSRPMVPIERGKAEWVVRGQDGTIICYGALLATCMEAVQRLNSQ
                     GLQFGLVNARFVKPLDADLICRVIQESPVVVTVEEGVLQGGFGSAVLEAANDAGLNTS
                     HLRRLGIPDRFIEHAERDELLSDLGLTAEGIAEACCALAAQVSRKPVRRSSARFSITG
                     HHS"
     gene            11404..12258
                     /locus_tag="PLD05_10710"
     CDS             11404..12258
                     /locus_tag="PLD05_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOP77954.1"
                     /translation="MKVIVLGYGGRADVVKEVERFLPIIERYAEILLVDLSGEKDLSP
                     YQADYVIVFGGDGSILRAARQMGKKQFPVIAVNLGKLGFLADISPQELEGVLSLAEHS
                     RLPVVDHLMLSSELVAQGSVVVQSLALNEVAILNGPPFGLLDVELWVDGDLATVYSGD
                     GLILATPVGSTAHSLSAGGPILRKDLDAIVICPISPHTLSNRPVVDSADRVYELRVLN
                     PQPNTALVIDGCTVGHVRADHRIYVRRADPRFQLVSAPGHSYYRTLREKLGWGGQTRA
                     NNRSTSSS"
     gene            12429..13379
                     /locus_tag="PLD05_10715"
     CDS             12429..13379
                     /locus_tag="PLD05_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77955.1"
                     /translation="MMVSFGCSLGSWLRSSVCGVALAAFWAGILMTGLTWGVNALGEE
                     ASGYRLRYRFQPGEELQWQVIHRARVRTTVAQTEQTAETLTRSTKTWKVLDVDGNGSA
                     RFELRVEDVEMTNRVSDGNSNGNEVRYDSKSGDPPPPGFADISKTIGKPLAIITLTSR
                     GEVIDRKQLEPEALQTHGYLTVPFPERPIKIGESWSVPDEVTIPLRNGTVKKVQIQQK
                     FTLEDVKNDVAVIRIGTQVLTPLNDPEVEVQLVQRLSEGTVRFDMAAGRMIAQEIEVD
                     RQVVGFQGEASAFHYQTRFTEEFLHSRMAQSPKPESTKQR"
     gene            complement(13424..>13663)
                     /locus_tag="PLD05_10720"
     CDS             complement(13424..>13663)
                     /locus_tag="PLD05_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77956.1"
                     /translation="MSLVTFTSVRISDLQIQVNKLTQPPTAEVRFFAVFRYEFKEPMP
                     EAIHDIYAARFKVLMEKVEDRWLLTDHYEYEIAHL"
BASE COUNT         3062 a         3433 c         3980 g         3088 t
ORIGIN      
        1 gcgatcgtcg gagccgatcg agaaaaccag cggggattcg agccaccctc accccgcgac
       61 ccctttcccg ccgaacgggt gaggggagat attatgggat aggctcttag tgaaaagacg
      121 accgagcgct cgtcatcgcc tatgaagctg aaaaagcagg gcagagggga acagacaagt
      181 ggcaggtcta gcgaagccgc aagatgtagc gcaagaattc actttccact tgtctcaagt
      241 cgccgaaagc ttgagaaaag tcttcaactc gctcttcagg ggaatcatat accaagggtg
      301 atttttctcg aaggcgggca agataggtga tgaaggcttg cccattctgc tgaaggagaa
      361 aataggtcaa ggcccaggcc tcggcatagg cgtccatggc cgtggaaagg ttttggaatc
      421 gtgaattgtc gcggatcaac gtcgctagtg agtcggatgg tcgttggggt aggtaggcgc
      481 ggaatcgctg caatcgttcg tagttcaatg cccccacggt gctccaaccc cggctgctcc
      541 gcaagtccgg cgtttcgaaa aacatcgcca aaccctctga gacccactgc ggtgtgtccg
      601 tccagcgaca ggtcacaccg gaattattgg caagttggtg cgacgcctca tggacaagcg
      661 ttgtgatggt ttgttgaact cccggttgca taatgtaccg tgcaatctga gccgctgtgg
      721 ccgtgccgtt gcttcctgtg agatcgtacg tggttacgac gttcgtctcc aggttgtagt
      781 gcccttgaat ccgctctatc gcatcaccaa cctcctcccg gcagtgctca atgtattgtt
      841 ggcgtgttgc aaacaccacg atcaccaacg ggaaagcagg acgttcgatg gtcagccctt
      901 tgtgcgaaaa gtaggccgtg aagccattgt aaaggcgttc caacagggaa ccgcaccagc
      961 gtgcgtaatc ccgggaggcg ttgtgcaaca caacataatg ggctgtggtg taaacctcaa
     1021 agtttttggg taatcgctga agccagtagg ttgcagcctc tttcggcgta gcggcctgaa
     1081 agggcaaatc attctgagtg atctcaatca cttcggatga tccgagcgga aggaggacgc
     1141 cgacgggggt caaaagaagg acgccgccat ctgcggcctg gacgaggatt tttccggtcg
     1201 cctcggtgat gccgttgtca ccgcgaaact tgaccacatc aattgcccag cctgaagaag
     1261 acagcgccag ggtaacgagg attgctccca ccatttttaa tgacgcgata aatcgaatcg
     1321 ctattttttg tggggatcgc gtggacatgg gccggaaatc acaacctgct ttcatgtggg
     1381 gcattcagtt gcaaaggcat gacccttctg gtggcgcggc atttagaaga tatcctggtc
     1441 ggaaagactc tcttcaaaac ggtccgcctg ccaggccagg gcaggaatga atcgggcaac
     1501 gtcaacaatg gtgagactat tcgcggggag tgacatctgg atgacgatga gttcttggtt
     1561 gttgaccttg tggaaggcaa aggccccaaa agtcaattgg gcgttatgtc gcagtaacat
     1621 ggcagcgttc tgctccgatg ctggaccaca taccgaccag aagcgaacaa tgttttgtcc
     1681 cccctctcga gattgttcgt agctgcacca gaccacctgc ttgcgaaggt taccaacggg
     1741 cacggtgatt ttgagcgtgt tgccgaattc ctccaggaca taaccagact gacgcgccac
     1801 ggtcttcacc aattgcaagg ggtcgctcgc accctcggag ggaaacccca gcggtggcgg
     1861 cgcggcttgt tggcccgctt tttccgcgag gggcaccgcc ggaggcacgg cctggggtgc
     1921 cggccccgca ggaccagttt gcaggggagc ttggccaccc gttgtgggca gcggaggtgg
     1981 cccagccgtc tcgcgcatag cctcccgaat catctgcggc aacatcatac cgaaaccagc
     2041 cccgtacccc attcccatgg ccccggcact ttcgccgccc gattcactta atttggtgag
     2101 gctctgcgct atctgatact tgagaaaggc gtctaggtcg ccgaccgccc ccatgccgct
     2161 ccgggcgtcg atcattcgtt ggacttcttc gggtggcgta atggcgttga taaagaaatc
     2221 gacaatctct aacccatatt tagcaaagtc tgccgccact tttgccctgg tccctgctga
     2281 aacctcgtca tacttggcag gcagatcgag gacgctgata ttgagcgtcc caagaaagtc
     2341 ggtgaggcga gcgacaatca aatctttcaa aaaattggta acttcctctg tagtgtattt
     2401 gccctgtgtt cccacgagcg tgttgattaa gaccgatgaa tctgccacgc gaaaggaata
     2461 cttgccaaaa cttcgcagac ggatcatccc aaattgggca tcgcgaaaga cgatcggttg
     2521 gcgggttccc catttctgat cgagaaacgt ctgtttaccg acaaaataga cggaacactg
     2581 aaatggcgac ttctcccagg gccacgtaaa caagcgggtc agtaagggga cgttcgctgt
     2641 ggtgagtgtg tatcgccccg gaccaaaccg atccaaagcc ttcccgtcac ggaaaaagac
     2701 cgcttcctgg ttttcataga cgatcaactg tgccccgagc ttgatatcgg ctgatccttc
     2761 tgcgggaatc cgctgaatca gtgaacgatt cgtttcgtcg aaaaactgaa taacttccag
     2821 acgcacaccc atcaaaggat ctcccaagtc tcaggtcttg tcgctgttcc caacttctat
     2881 tcgtgaattg gctactgaaa tttgcaagac tcaccgcaaa cccgccaaaa gctcgtgccg
     2941 catatcccag agccggtgaa gctcatctaa cgattggcgg acctcaccaa tggcgcagga
     3001 cagatcggtg gggcatctcg ggagtcgttc caccgcttca gcgagttgtt ccacgcgttc
     3061 tgcaagggac agatcgaact ggtacacgcg atcaaggacc tcttcgtcaa tctggactag
     3121 gtcgaagaaa ccgctgtaac cttccatcgc cccgcggatc ctggcaacga tgtggtcaat
     3181 tcggccgcgg acctgttcca atgtggccaa ctgatcgacc tgtccgcctt ccgcaagaag
     3241 gcccatcaag ttatcgacgg agcacttggc caactccaag cgatgagcca accattcccg
     3301 ttggaggttg tcgctgtcac gccggtattc tttttccaaa tacccgcgaa aaccgggaat
     3361 gtgtcgcagg atcgtctcaa cccaatttcg tttcttcgtg tgatccatag gttccaccat
     3421 ggcgttaccc tcctctttcc cagccagacg atatctgaac cgacgcactg gttcaggccg
     3481 accaccgaac gtagacgatc cccaccgatg tccaaggctg cctttatcat atcacatcaa
     3541 tggggtaagg gcgaatcacc aaaaatgggt tcccgcctga aaatctggga tgcctacgct
     3601 ttttccgatt tcttctcttg gtcagtcaag acgaaattgg tataagagcc tatcccgtaa
     3661 tcttccctca cgcgttcggc gggagagggg ccgggggtga aggtggctcg aatcgttgcc
     3721 ggttttctcg attaatttcg acgttcgctt gccctcaccc cagccctctc ccgggagaga
     3781 gggggtcttt ggggtaagct ctaagttacg cgccggaccg cggcatgcga ggaatcctgt
     3841 ttgatagctt caatcgcccc taaaagatcg aacagcgaca atcggtcccg aaaaagtacc
     3901 atgaacacgc aaagatcagc gagccgaact catcaccaaa ctaaatgcca aatcggtttg
     3961 atgtcgttat tggcatacct cgcggtaggg gcgtgcttgt gcggctgtcg gacaggtttc
     4021 ctatcggttc agaagaaggc ccggcaggaa ctcccgccgg gtatcgtgct accccgggaa
     4081 aagatagaga aatggaggga actggtcaat tcagcaaagg attcagggtc tgcggcaacg
     4141 gcaacggctc gtttacaaga ggccttccaa ggtgaagatg acccccagtt aagggcagaa
     4201 ctggtccgat tggccggttc gttgccacct gaagccgcct ggccgattgt cgagcttgga
     4261 ctccgcgacg aaaaccccga cgtccaagtt caggcctgcc gaatggttgc taaggcgaag
     4321 atggctcagg ccgtgggctc cctcgcgaaa ctggcggagg aatcaagtaa ccaggatgtc
     4381 cgccaagcgg cgattaaggc cctcgggcaa ttcaaggatc cggtggccat tcaagcctta
     4441 gcaaaggccc tccgagatcg tgaccccgcc attcaatacc tggccattca ctcgcttcgg
     4501 acggtcacgg ggcaggattt tggatacgac gttgcccaat ggttggcgtt tttggagggc
     4561 cggcaaaacc cgccccaacc tactcgggcc atggctaccc gaccgacaca gcgaaggtga
     4621 tccggttttg gggagattgt gccagcagga tagtcctgaa atctggtggc gtgcttacaa
     4681 aaagcaacgc ttttgaatgt ggatcggcca caaagaaata tcgcactggt cagttaaaat
     4741 tggcgattca cgctctttcg tgtagtccct tccatgacct gagcaatcat ctcgccggac
     4801 aatcattaaa gggctgtttt ggtcattcga cataggaaac ggtgttggca ctgattttct
     4861 cagggcaaat cggtaaactg ggtgtttttg gcttggtagg tccctttttg gcccacgtgg
     4921 ttgtggaaat gtggtcctag tcattggcgg atgaaaagcg caaaatggag ggttatcgac
     4981 cgccctgtgg tcggtggtcg caaccatgcc atctggagtc ttggattcag accacgtctt
     5041 taggcctcgg accccgaagg tgaagggaac gtcggttcac gattggtagt acctgctttc
     5101 aagagaggaa gtgaaacatg agttcaacga ttgttgacat cctcggacga caaatcctag
     5161 atagccgcgg taaccccacg gtggaagtcg aagtgcttct ggaagacggc tcacttggtc
     5221 gggcagccgt tccgagtggt gccagcacgg ggacgcgcga agccctggag ttgcgggatg
     5281 gcgatccatc aaagtatctc gggaaggggg tctcgcaggc cgttcaaaat gttaatgaaa
     5341 aaattgcaga agagatttgt ggcatggatg cgctcgatca agccgccatt gattatgcga
     5401 tgatcgagct ggatggcaca gagaataaga gcaatctcgg ggccaacgcc attcttggtg
     5461 tttcgttggc cgtggctcat gcggccgcgc agagcagcgg cttgcccctg tatcgttatc
     5521 ttggtggcgc gcttgcccgg gttgtgccgg cacctatgat gaacatcctt aatggtgggg
     5581 cccatgccga caatgatgtg gatatccaag agttcatggt catgcctctt ggatttgaga
     5641 cattttccga tgccatccgc tgcggttgcg aaatcttcca taacctgcga aaggtcctta
     5701 aggatcgaaa actgtcgaca aatgtgggtg atgagggtgg ctttgcgccg gacctgaaaa
     5761 gcaatgaaga ggccctggag tgcatcaagg aggcaattga tcgggcaggc taccaattgg
     5821 gcgagcaagt ctttttggcc ctagacttgg ctgcgagcga gttggccgtc gaggggaaga
     5881 agggcatgta catgatcggg ggcaaagagg tgccggccaa ggccgtgatc gacatgctgg
     5941 aatcctggct ggagaaatat tcgatctgct cgattgagga tgcttgttct gaggacgatt
     6001 gggacggctg ggccttgctc acggagcggc tcggaaagaa ggtgcaaatc gttggtgatg
     6061 acgtcttcgt aacgaacaca aaaatctttg caaagggtat tgagtcgggt attgccaaca
     6121 gcattctgat taaggttaac cagatcggaa cgctgacgga gacgttcgag tgcatcgata
     6181 tggcccgtcg cgccggttat acgtatgtca tcagtcaccg gagtggcgag actgaggata
     6241 ctacgatcgc agacatcgcg gtggcagcgg gtggcgggca gatcaagacg ggttcggctt
     6301 cgcggactga ccgtatcgca aagtataatc aactgttgcg gatcgaagag atgttaggtt
     6361 ccactgcgat ctacggtggc aagctctttc cgaaacagtg gaaatagctt acatgcggca
     6421 aggttttccg cgaaacgttc agggtgtttt ccctgtcacg cagccatcgt ggtaagtgag
     6481 ggggcgaaat tgtcaggcga gaatcggtcg aggcgttcct gcgaggggcg ccttgttttg
     6541 ttttttgcac ataagtgcgc cggagatggt gacttggagc cagacgtatg atctggcgta
     6601 cgtccaccga ccgagttttg gcgaggtaca cgcattgtag ctgggattat gacaaatcca
     6661 acagaagaaa agattctcgt tctcgatttt gggtcgcaat acgcgcagct cattgccagg
     6721 cgcgtccgag aacaaaacgt ctttgccgtg attgtccgtc atgacatttc ggcagaacgg
     6781 gttcaggaat tggcacccaa gggactgatt ttctcgggcg ggccggccag cgttcactgg
     6841 tccgatagcc caaagtgtga tcccgatcta tttcagcttg atatccccat tttggggata
     6901 tgttatggaa tgcaactcgt ctgtcagtcc atgggtggta aggtggaaag tgatcggggg
     6961 cgtgaatatg gccccgcgat ctgccgggtc atccagcaga atgaactgtt taccgatctg
     7021 ccatccgagt ttcaagtttg gatgagccac ggcgatcaag tgacctcgat ttccgaagag
     7081 ttcgttgtcc tggcagctac ggaaagttgc cccttagcgg cagtaaggca tcgcagccgg
     7141 ccactctacg gccttcaatt tcatccggag gtcacccata cgacttacgg ttcgcaaatc
     7201 ctgcgcaact ttgttcacaa ggtatgccag tgcagcggga cgtggaagtt aagcgatttt
     7261 gccgaggaga caatcaaaga aattcgccag cgggttgggc gtgatcgcgt catttgtggc
     7321 ctgtccggag gggtggactc gtccgtgaca gcagctctgt tgtcgcgggc tgtgggagac
     7381 cagacggcct gtatcctcgt ggataatgga ttacttaggg aaggagaggt cgaatcggtc
     7441 atcgaggagt tcacaaagca tttccaaaca gacctgcacg ttgttcgcgc agaggatcgc
     7501 tttttggcgg ctttgcgagg agttgtgaat cctcaggaga agcggaagat cattggacgt
     7561 gtgttcgtgg attgctttgc cgaggaagca gcgaagattc ccaatgcccg gtttctggcc
     7621 caaggaacgc tttaccctga tgtcatcgaa agcgggggat tgcgagacgg tcctgccgcc
     7681 accatcaagt tacatcacaa cgttggggga ctgcctgaag acctgcaatt tgaattgatc
     7741 gaacccttac gcaatctgtt taaggacgag gtgcggaacc tcggcatgca acttggtttg
     7801 cccgacgaaa tcgtctggcg gcacccattt cctgggccag ggcttgccgt tcgttgtatc
     7861 ggagaagtta caaaagagcg acttaatatc ctccgccaag cggatgccat agtgatcgaa
     7921 gaaatcaaac gggcggggtg gtaccgaaaa acctctcagg ccttcgctgt gttgctgcct
     7981 gttcagacag tgggtgtgat gggtgacgcc cgaacgtatg aggaagtcat agccgtgcgg
     8041 gcagtggata ccgaagactt tatgacggca gattggtcga agttgcccta cgaattgttg
     8101 gcacggattt caacgcggat tatcaacgag gtttccggaa tcaaccgcgt ggtttacgat
     8161 atcagttcta agccccctgc caccattgag tgggagtaag cctaggcaga gtagggggaa
     8221 cgcaggtcgc agaagcggca aaaaacacat tggatggatg gagtaggtag cacgctggta
     8281 tctttggaag tgaatgaatg tgagtgagtc agatcgctgc ccttctggtg aggaatcgac
     8341 accccgattc gaggacgcgc tgcgtgagtt agaggcgatc gtggaacggc tcgaacgggg
     8401 tcagctcggc ctggatgagg cgttggccga gtacgaacgt ggggtccgcc tgattcggca
     8461 ctgccgggag cttttgcaga aggccgaacg gcggatcgaa attctctctg gaatcgacga
     8521 gcaacagcga cctatcacgc agccttttga agaggaagac ctttcgctcg aagagaagcg
     8581 agaaaaacgg agtcgccgcc gcggggtgaa gaacctttcc gatgaagagt cggagagtga
     8641 ggctgggtga gagatcgcta gggcagggga gcgcaaaggg attggtttta tccacgagtt
     8701 ttatccacga ttcgcagcaa ccacggggga caaggtcggc atggacttcg cgctcaattg
     8761 gcaagaaacg gaaaagcatt tggccgagct tcgtgatttg gtcaaccaat ggctcgataa
     8821 actaacggac ttgccggatg gttgccctga tcgactgaag gctgccatga gatacagtct
     8881 tctagcgcca ggcaaacgac tgcggccaac gctcgttctt ttggcggcgg aggcctgcgg
     8941 cgccgatccc gaggaggcta tcgccccggc atgcgccatc gaaatgattc actgctattc
     9001 gctcattcac gatgacttgc cggccatgga caatgatgac ctccgccgag gacagccaac
     9061 ctgtcaccga cagtttgatg aggcgacggc gatcctggct ggcgatgcgc tattgacgct
     9121 tgcgttcgaa actctttcaa ggtacatgga accagcagat cgggccgtcc aatgctgtcg
     9181 ccttctggcg gaggctgctg gaccgtgtca gcttgtgggc ggtcaggccg acgatatcca
     9241 atcccccaca ttaaccccga cacttgacct gctggagcgg attcaccgta gaaagacagg
     9301 agcactgatc gagtgtgctt taaagatggg cggaattgtc gggagcgcca attctgaggt
     9361 tttaaacggc ctttcggaat acggtcgtgc gatcggtttg gcatttcaaa ttacggacga
     9421 cttgcttgat ctgcttggtg atgaaaaact agttggtaag cggcttcaaa aagataataa
     9481 gcagaaaaag ttgactttcc cgtccttgtt gggggttgaa gaaagtcgta agaggctaga
     9541 tgagttaatt gaggctgcat gccgcgtcct tgaaggctta cccattgagc gacggcattt
     9601 ggtggccttg gccataaatc tcaagtcacg gacccactga tggataagct tctgtcgaag
     9661 atacagtccc cgcacgatct tcagtcattg acgagtgccc aactggaaca attggctgcc
     9721 gagatgcgcg aagcgatgtg ccagttgttg gggactcgct ccgcccattt tgcgtcaaat
     9781 ctgggggttg tcgagttaac cattgccctt cacacgactt ttgatttctc gcgtgaccga
     9841 ttaatttggg ataccggaca ccaggtctat cctcacaaga tggtgaccgg ccggtatcgt
     9901 cagttccaca cgatccggac taagggaggg ctcatgggct accccaaccc ggcggagagc
     9961 ccctatgacc tctttatgac aggtcacgct ggttgcagca tctcgacggc catcggtttg
    10021 aaatgcggtg atgacctgct tcggcccgat gagaatcgct atgttgtggc cgtgattggg
    10081 gatggggcgt ttccgtcggg gattgtttca gaggccatga atcatgccgg ttggctaaag
    10141 aaacggctca tcgtgatttt gaacgataat cgcatgtcga tctgccagcg cgtgggcggc
    10201 attgcggaat acctcgatcg actgcgaatg gcccccctgt attctgggat caagaatgag
    10261 gtctataacc ttctgtcaaa agttccattg gtcgggaact caatggagcg gctcctcgat
    10321 cagttgcgcg acgccattaa ggcggggatg ctggggggaa tgctttttga ggagttggga
    10381 ttccgttacc tcggtccggt ggatgggcat aatattgggc agcttcaaaa atacttgagg
    10441 atggtcaagc aattccccgg cccggtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnggc atatgcgagt ctcatgccgt gggctttgcc gcggggctgg ccaaagcggg
    10621 gctgcgtccg attgtggata tttacagcac ctttctacag cgggcgtatg atcaactctt
    10681 tcaagaggcg gcccttcaga acctgccgat tgtgtttatg atggatcggg cgggggtggt
    10741 tggggctgat ggcccaacgc atcatggggt ttttgatatt gcctatttca ggcattttcc
    10801 caatatgacg gttctcgccc cgggtgatgc ggacgaactt gaggccatgc ttgccgttgc
    10861 cctccagcag gaggggcctg tcgccatccg atacccaaaa gcgacggcga ccgttgtctc
    10921 ccggccgatg gttcccatag aacgcgggaa ggcagaatgg gtcgtccgtg gccaggacgg
    10981 gaccatcata tgctacggcg cgctccttgc gacgtgcatg gaagccgtgc agcgcctcaa
    11041 ttcgcagggg cttcaatttg ggctggtcaa tgcgcggttt gtcaaaccct tggacgccga
    11101 tttgatttgc cgtgtcatcc aggagagccc ggtggtcgtg acggtggagg agggtgtact
    11161 tcaaggggga ttcggaagtg cggtcctgga agcagcaaac gatgcggggc ttaatacctc
    11221 gcatcttcgc agattgggta tccccgaccg gtttattgaa catgcggaac gagacgaact
    11281 gctgtccgat ctcggtctga cggcggaggg aattgcggag gcctgttgcg ccttggcggc
    11341 acaggtgtcg cggaagcctg tacgtcggtc atccgcccgt ttttctatca ctgggcatca
    11401 ctcatgaaag tcatcgtcct tggctacggt ggtcgagctg atgtcgtcaa agaggttgaa
    11461 cgatttctgc cgatcatcga gcgctacgcc gagatcttgc tcgtcgattt gtccggtgaa
    11521 aaggaccttt ctccgtacca ggccgactac gtcatcgttt ttggtggtga cggatccatc
    11581 ctgcgggcag cgcgacaaat gggaaagaag caatttcccg taatcgcggt taacttggga
    11641 aagctggggt ttttggcgga catttcgcct caggaattgg aaggcgtgct ttctttggct
    11701 gagcacagtc gcttgcccgt cgtggatcat ttgatgctct ccagcgagct tgtggctcaa
    11761 ggaagtgtcg tagttcaatc attggccctc aatgaggtgg caattctcaa cggtccgcca
    11821 tttggcttac ttgacgtcga gttgtgggtg gatggtgatc tcgccacagt ttactccgga
    11881 gatgggctga ttctggcaac gccagttggt tcgacagccc attccctttc ggcaggtggt
    11941 cccattcttc gcaaagacct agatgcgatt gtgatctgcc cgattagtcc ccacacgctt
    12001 tccaatcgtc cggtcgttga ttcggcagat cgtgtgtacg agttacgggt cctgaatccc
    12061 caacccaaca ccgcgctggt gatcgacggc tgcactgtcg gccatgtccg cgccgatcac
    12121 cgcatctacg tccggcgagc cgatccacgg tttcaattag tgagtgctcc tggacatagc
    12181 tattaccgaa cactccgcga gaaattgggc tggggtggcc agacaagggc aaacaatcgg
    12241 tcaacgtcct cttcctaggt tgcccagtct cttgatcttt cacgaaggcc ggtaatggga
    12301 gaccacacgg ccttcaaaat gatcttttca ggcccaagct acagggttat aaacaggcat
    12361 gaagacttgg agggtcttcg tcctgcggcg tggaagtttc acggaattga aagaaaagag
    12421 ttattgccat gatggtatct tttggatgtt ctcttggctc gtggctccgg tcatcagtgt
    12481 gcggagttgc attggcggct ttctgggccg gcatcctgat gacggggtta acctggggag
    12541 tgaatgcgtt aggcgaggag gcatctggtt atcgcttgcg ttatcgcttt caacccgggg
    12601 aggaacttca atggcaagtg attcaccggg ccagggtaag gacgacggta gctcaaacgg
    12661 agcagacagc cgagacgctg acccgttcga caaagacttg gaaggtctta gacgtggatg
    12721 gtaacggttc tgcgagattt gagttgcgcg tcgaagacgt ggaaatgaca aatcgtgtca
    12781 gcgatggtaa cagcaatggc aacgaagtgc ggtacgacag caagtccggc gacccaccgc
    12841 caccgggttt tgccgacatc agcaaaacca tcggtaaacc gctggccatc atcacgttga
    12901 catctcgcgg agaggtcatc gatcggaaac agctcgagcc ggaggctttg cagacccacg
    12961 gatatttgac agtccctttt ccggagcgcc ccatcaagat aggagagtcg tggagtgttc
    13021 ccgatgaggt caccattccg ctacgaaatg gaactgtgaa aaaagttcaa atccaacaga
    13081 agttcaccct cgaggacgtc aaaaacgacg tggccgtgat tcgcattgga acgcaggtcc
    13141 tgactcccct caatgatccc gaggttgagg tccaactcgt tcagcgcctc agtgagggga
    13201 ctgttcgctt tgatatggcg gccggacgaa tgatcgcgca ggagatcgag gtcgatcgac
    13261 aggtggtcgg ctttcagggt gaggcaagcg ccttccacta ccagacgcgg ttcacggagg
    13321 agttcctcca tagccgaatg gcccagagcc ccaagccaga gagcacgaag caacggtaga
    13381 tgctggctat tttccaaaac acgctaacca gtggaacgcc gtatcagaga tgggcaattt
    13441 cgtattcata gtggtctgtt aataaccagc ggtcttcgac cttttccatc agcaccttga
    13501 atcttgccgc gtaaatgtcg tggatggctt ctggcatggg ctctttgaac tcgtaacgaa
    13561 acacggcaaa aaaccggact tctgccgtcg gaggctgggt tagcttgttg acctgaattt
    13621 ggagatctga aattcggaca ctggtgaagg tgacaaggga cat
//
LOCUS       DAOSUT010000111        13616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2705_length_13616_cov_10.615589, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000111 DAOSUT010000000
VERSION     DAOSUT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2705_length_13616_cov_10.615589"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            795..1904
                     /locus_tag="PLD05_10725"
     CDS             795..1904
                     /locus_tag="PLD05_10725"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOP77957.1"
                     /translation="MICPRCHTWLSQVKRPQGLVYQCASCRGFAVSIPVLRKLVPQGI
                     IRDLWNRAQEKQSRSGAECPVCRRKMADVAVGGMEKAPIIDVCIRCQFVWFDRRELEQ
                     LPPVIVKQPDRETLPLEVREKLAVLELERLKRREQLATAGDQYPEEWWQWIPGLLGLP
                     VELDAPAVRSVPWLTWSITAAMVVTFLLTFNHLETILDVLGLKPAEFMRLGGLTLITS
                     FFLHGSFWHLLGNGYFLLIFGDQVEDDLGQWRFAALLALAALAGDILHILGDPRSTVP
                     CIGASGGISGIIAFYALRFPSARLGIMLRYFLIFRWIQFPAWAGFVAWLVLQIILAIE
                     QVQGVTNVSALAHLGGCLVGVGAWGLWQSGLLKAG"
     gene            2199..3899
                     /locus_tag="PLD05_10730"
     CDS             2199..3899
                     /locus_tag="PLD05_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP77958.1"
                     /translation="MPAWDATLHWLKTTGNRAAGEVLIRALDSPCPEIQRGAVFALLA
                     RREMAGHRVILERIDPASEEWRGQLNSLPSQFLASLRRFLLDGDPPLREKVCHFLRET
                     EQFEFVPFLANLLENREYEPRLVVANTLSELCRRLHQRLDQPDNGQRGRHEERIAEQL
                     RRSLLVSVGRFPRHECREILDGLLLLTEEHPELITAIINNRGHPARRLLLEILSRETG
                     AHVLAVVCYCLHQEAPPGDLVAIIGQRTDLPFVRHLLSTVGPQPSSIVQRNLKRFYQF
                     RWLKDLPNLLGQLDDTEQQAIPALVAHSRIPRDEALKVIGHVLLHGRPAGRRAAAKAL
                     AGFHGVEANAVTVRAMRDPDPGVQEAIIPQLRQRGILGALPYIISKLESPHPMVRRAA
                     RKSLREFTFTRFLQVWDMLPPEVRRETGVLVKRVDPRTTLLLRAELLSTFRLRRLRAL
                     EIAQELELVPRLESSIICLLRDDDPAIRAEAAAALAHTDSAASYQALLETLNDANVFV
                     RNIAEVTLEKRRRRMESQEQGQKNAPSGESQPRQPQPSGIISDETNVSHLDTSPTTTS
                     "
     gene            3896..4519
                     /locus_tag="PLD05_10735"
     CDS             3896..4519
                     /locus_tag="PLD05_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77959.1"
                     /translation="MTSPTMRRFTQALLARAIWRLGHVALVQAVAWVITTFLVYGSEA
                     AAYGQGRTPLEILHREFQRRATGSDTDHTALLWGLAVFVGLVAVLMIIAWVINYFQQR
                     RPYFSHTLLFLELCCVHRMRIKDILLLWKWTSREKIRPRSLIFVDPGLWQNKIDHNLL
                     NVTAGSIAAQTTWNRLYTQLFGAFVPQPSENFSPRETARGPTTSCEN"
     gene            complement(4586..5905)
                     /locus_tag="PLD05_10740"
     CDS             complement(4586..5905)
                     /locus_tag="PLD05_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP77960.1"
                     /translation="MNQNASQGDTTRRDFLQATAAGGAVLTWPWTRFVHAAGSDVVKI
                     ALSGCGGRGTGAVSDAMVNKVGAKVKLVAVHDAFKERVDASLHALKGRFGEMADVPPD
                     RQFTGLDGYLKLMETDADIVLLCSPPGFRPMHYEAAVKAGKHVFMEKPLAVDAPGVRR
                     IMATNEEAKKKGLRVAVGHHLRHEVKHKEMIQRIWDGEIGELMYMRAYFNSRGVWVRP
                     RKPEQTEMQYQVNNWYYFTWLSGDHIVEQHVHDLDVLNWMMGNKHPISAQGTGGRQFR
                     IGKDYGEIYDHHAVEFVYENGVRAYSYCRHIPGCWDSFSEHAHGTKGYVSIEGHGHAI
                     IHVQGKDPVRVERGPDGHQLEWNDFLQAHMAGQAYNEVDATILGTMTAILGRMATYSG
                     QIVTWDEAIKSELDLSPSGYTWDSVPQPKPGPDGIYPCAIPGQTKAY"
     gene            complement(6252..7082)
                     /locus_tag="PLD05_10745"
     CDS             complement(6252..7082)
                     /locus_tag="PLD05_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOP77961.1"
                     /translation="MLPNAIPLDTRVLILCAGEGERWGEYLGKPKQLAPIFGVPLLVR
                     TAGQVQHYFGVSPVIVTHDPRLHIPGCDSFCPEQRRWVVETFLSTACLWSSRTVVLLG
                     NVLYTDAAITICRAASGGLVIFGRQGASIFTGKRYGELFGLSFTRDYSDFVTHCLIQV
                     IGDATRGGRGKLWEFYFLAQREPPSGRPPIEFCEIHDYTEDFDSPEEYHYLKRLCEWA
                     LSSAPALRLLAILWSSVFCAPYASWHRWKQWWHDRRNTRRTTLPLSRPQTSPSHRAAA
                     "
     gene            complement(7313..8434)
                     /gene="mnmA"
                     /locus_tag="PLD05_10750"
     CDS             complement(7313..8434)
                     /gene="mnmA"
                     /locus_tag="PLD05_10750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOP77962.1"
                     /translation="MARVLVAMSGGVDSSVSAHLLKEQGHEVIGVFMRHGIRSPLHCV
                     NEGRNPTLPVLPGRKQGCCSAEDAEDARRVADRLGIPFYVVNFEEEFRQIVHYFVDEY
                     TRGRTPNPCIVCNNRLKFGKLFDYADALQADFIATGHYAQIIPTEDKNYGLFCGRDQS
                     KDQSYVLFGLRREDLPRLLFPIGNYTKAEIRKIATELGLRVANKPDSQEICFVPDKDH
                     ARFVKQYRPNVDTSGEIVTTRGEVVGHHGGLERFTIGQRKGLGIAFGEPRYVVRIEAD
                     TRRVVVGTREELARWDLTASGANWLIDPPAEPFPCQVKIRYRTPAVPATVTILSSDRF
                     LVRFHKPCYGVAPGQAAVCYQGTRVLGGGWIDSSPASNG"
     gene            complement(8663..9388)
                     /locus_tag="PLD05_10755"
     CDS             complement(8663..9388)
                     /locus_tag="PLD05_10755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOP77963.1"
                     /translation="MVTAWRKTQLAIKRGIDIIGAAVALIILSPVMAATALAVWITMG
                     RPIIFRQVRPGYRGRLFVLYKFRTMRNAWSADGQPLPDAERLTPLGRWLRRFSLDELP
                     QLWNVLKGDMSFVGPRPLLVEYLPRYTPEQARRHEMRPGITGLAQVKGRAALKFSQRL
                     AYDVYYVDHFNLLLDLKIILLTLWKVPMGLLFDGAGQDVKVVDDIGLHPGQETLTWKL
                     SATGETQLKLSDQTADTAKRSAA"
     gene            9777..11795
                     /locus_tag="PLD05_10760"
     CDS             9777..11795
                     /locus_tag="PLD05_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOP77964.1"
                     /translation="MDRCVLRRCWTVLLTAILAVTCGCALRHGGHVRPWAKPGLFLRP
                     QMLSQSPTSLTSRTIQFLRQHNLDSSLHEDPRTLLQQVQAVIQKEPQADNVYAYAEIS
                     YVLAKRVERSQPRLALDFYGGTVLKAYQYLFDPKFEDTRNPYDPRFRGACDLYNSALE
                     AALRIVRANESLQPGSAFSVRTADGYWTLTCTICDRRWRAEDFGTFEFVSDYRITGLR
                     NHHVHYGLGVPMIAVRKNYPGEPPAARYYPPDLSFPVTVFLRPEIPSATESQEEDRHY
                     EGRLELYDPVATMNIQVGNYVVPLQADLTTPLATFLSNPALGSSLATVGLLRPEKLLT
                     LQPGLNKPLTGLYMVQPYEPDKIPVIMIHGWWSSPMTWIQMFNDLRSDPLIRRRYQFW
                     FYLYPTGQPFWISAAQFRRDLRELRQTLDPDHRQPALDQIVLIGHSMGGLIAELQTID
                     SGQDFWKLVSEHPFEEIRTDPISREELREIFFFQPNPSIRRIITIATPHLGSHFSNQM
                     TQRLAARLISVPKELLQSQTQFFRENQDLLLPNNILQITDSVDALDPDCPIFALMNQA
                     RRASWIVHHNIVGASLEDRLWGRITGTSDGVISWESAHSDKADSEIAVPAEHTSVQSH
                     PLTILEVKRILLEHLAELHQEHSLFTSGALPADVAVPSPVRLPPVEYP"
     gene            11884..13329
                     /locus_tag="PLD05_10765"
     CDS             11884..13329
                     /locus_tag="PLD05_10765"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOP77965.1"
                     /translation="MSMTYRIETDSMGEVRLPANAYYGAQTQRAVENFPISGQTLPPE
                     LIHALGLVKWAAAQANYDLGLLTRGPRALNPAQVQALLQACQEVAEARFDDQFPVDVY
                     QTGSGTSSNMNVNEVIANRAIELSGGDRFQSEKPIHPNDHVNMGQSTNDMFPTAIHVA
                     LAVAIHRDLIPALKEARSILEEKAERWGDIIKIGRTHLADATPLTLGQEMSGLARMME
                     LAAERAQRGVDALMELPAGGTAVGTGINTHPDFGRRVTAFLAEKTGLPFREAVNHFEA
                     NAQRDGLVECHGQIRAVAVSVFNVANNIRWLSSGPRCGFYEIILPELQPGSSIMPGKV
                     NPVLCESLMQIAARVIGNDQTIAFAGASGGQFQLNIMMPVMAHVALESVRLLARGVRI
                     FAEKTLRGMQANREACEAAVEKSLALATSLNPIVGYQKAAAIAKEAFKTGKTIRQICS
                     EQKILSPEQLAEVLDPLKMIRPRGDDPSSPA"
BASE COUNT         2960 a         3921 c         3687 g         3048 t
ORIGIN      
        1 ttagagcagc ggaatcataa tccgcgtgtc cggggttcga gtccctgcgc cggcaatgac
       61 agcgggccac acaatatcgt gtgcgcgtaa ttgatacttc agggtctatc ctaaaaagtc
      121 cccctctccc tccgggagag ggttggggtg agggtaagga accgtcggag tcgatcgaga
      181 aaaccggcag ggattccagc cactctcatc cccgatccct gtcccgccga acgggtgaag
      241 ggagattatg ggatggcttt aagcccgtca caggatctct gtcagttgtg tcaggcggta
      301 gtgacaaaaa tggcaaggcc tgacggcttg gggcttggag agtcccacct gatcatttgg
      361 caattatcat cgctgcgaac agagaccctc gtcgctggaa tcgccggttt aggctgattt
      421 agcaatttgg gccataatgt acccccctag ggtgcggtta cgccatcccg ccgtttgccg
      481 gggaccatta aaaaagtgtg cccgcctgaa atgggcactc aacctcgttc tcattggcgc
      541 taaggcctca catgccttat taggaacaca atcctcggac ggttctcatc ctgctgcggg
      601 ggctttctga cggtttacga cacgccggct aagaccgtgt ccgaacaagg attgggccag
      661 tttcctaggc tggtggggca tcggcccgcc gggatataat ggcggcggtc tcctaagcgg
      721 acggggacgg gaccaaacgg cgcgaagcga atcggataca ccggccgcgc ggccaatcgc
      781 aaaacttatt cacaatgatt tgcccacgct gccatacctg gctaagtcaa gtgaagcgcc
      841 ctcaggggtt ggtgtatcaa tgcgcttcat gtcggggatt tgccgtctcg atacccgtgt
      901 tgcggaagct cgttcctcag ggcatcatcc gagatctttg gaaccgtgcc caggaaaaac
      961 agtcccgctc tggtgccgaa tgtcccgttt gccgccgcaa gatggctgac gtcgctgtcg
     1021 gcgggatgga gaaggcaccc attattgatg tttgcatccg gtgtcaattc gtttggttcg
     1081 ataggcgaga actcgagcag ttgccaccgg taatagtgaa acaacctgac cgggagaccc
     1141 tgccgctaga ggtgagagaa aaattagccg tcttggaact ggagcgactc aagaggcgag
     1201 agcagttagc gactgcaggc gaccaatacc cggaggagtg gtggcaatgg atccccgggc
     1261 ttctcggatt gccggtggaa ctggatgcac ccgctgtccg ctctgtgccc tggctgacgt
     1321 ggtccattac ggccgccatg gtggtgacgt ttttgctcac gtttaaccac cttgaaacca
     1381 tcctcgatgt gcttgggctc aaacctgccg agtttatgcg gctcggcgga ttaaccctaa
     1441 tcactagctt ttttcttcat gggagttttt ggcatctttt gggcaacggt tattttctcc
     1501 tcatctttgg ggaccaagta gaggacgatc tagggcagtg gcgttttgcg gcgttattgg
     1561 ccttggccgc actggcaggc gatattttac atatcctcgg tgaccctcgc agtacggtac
     1621 catgtatcgg ggccagtggt ggtatttcgg ggatcattgc gttttacgcc ttgaggtttc
     1681 ccagcgctcg tctagggatc atgttacgtt attttttgat ctttcggtgg attcaattcc
     1741 cggcctgggc tggctttgtc gcctggttgg ttcttcagat catcctggcc atcgaacagg
     1801 ttcagggcgt aaccaacgtt tcggcactcg cccacttagg tggttgcctc gtcggtgttg
     1861 gggcgtgggg cctttggcag agcggcttgc taaaggcggg ttaaagctcg cgaagctctc
     1921 cccgacccct tgactggcga tttgaaatgc caatgggaga accaaatagc aatctccccc
     1981 tacgcccgta agcgaccgct gcccataaag ccgtcccacc tggtgggcaa tcaagataca
     2041 ccagcccgag caaggcgcaa aaacggccga ccacgagggg ctcccggcaa ttatatctca
     2101 caaatttggc gcatggtgcc ttatgatgac gtagattctt atggaggaat tactgtttct
     2161 tgagagaacc gctatcccaa cgaccaaggc agggggctat gcccgcctgg gatgcaacac
     2221 ttcactggct gaagacaacg ggaaaccgcg cggcggggga agtcctcatt cgcgccctcg
     2281 attcgccgtg tcctgagatc cagaggggtg cggtcttcgc cttgttggca cgaagggaga
     2341 tggcgggcca tcgggtgatc ctggagcgaa ttgacccggc ctctgaggag tggcgagggc
     2401 aacttaacag ccttccgtct cagttcctgg cttcattaag aaggttttta ctcgacgggg
     2461 acccacctct gcgagagaag gtttgtcatt ttttgcgaga gacagagcaa ttcgagtttg
     2521 tgcccttctt ggccaatctc ctggagaacc gcgaatacga accacgattg gttgtcgcca
     2581 ataccctttc ggagctgtgc cggcgattgc accagcgcct cgaccagccg gataacggtc
     2641 aacgtggccg acatgaggaa cggatagccg agcaattacg tcgatccctg cttgtgtcgg
     2701 tggggcgatt tcctcggcat gaatgccgtg aaatcctgga tggcttgtta ctgttaacgg
     2761 aagaacatcc ggagttaatc accgccatca tcaataaccg gggacaccca gcacgtcgtc
     2821 tcctcctcga aatcctatcc cgcgaaacgg gtgcacatgt cttggctgtt gtgtgctact
     2881 gtctccatca agaggcgcct cctggggatc ttgtggccat catcggtcag cgaaccgatt
     2941 tgccgtttgt tcggcatcta ttatcaacgg tcggaccgca accaagttct atcgtgcaac
     3001 ggaatctgaa acggttttac caattccgtt ggcttaaaga cctccctaac ctgttaggcc
     3061 aactggacga tacggaacag caggccatac cggcgcttgt tgcccattcg cgaattcccc
     3121 gggatgaggc actgaaggtc attggtcacg tgctcctaca tggacgaccg gcagggcggc
     3181 gggccgcagc aaaggccctc gcgggcttcc acggcgtcga agcaaatgcc gtcactgtcc
     3241 gggcgatgcg agatcctgac cccggggtac aagaggccat tattccccaa ttgcggcaac
     3301 gcgggattct aggagcgttg ccctacatta tcagcaaact ggaaagccct caccctatgg
     3361 ttcgtcgggc ggctcgaaag agtcttcggg agttcacgtt tacacgattc cttcaagtgt
     3421 gggacatgct tccgccggaa gtgcggcgcg aaaccggcgt ccttgtcaaa cgagttgatc
     3481 cccgcacgac cctcctgctt cgtgcagaat tgctttccac cttccgttta cggcgtctcc
     3541 gagccctgga aattgctcag gaacttgaac tggtgccccg attggagtcg tcaattatct
     3601 gtctccttcg agatgatgac cctgcgatcc gggcagaggc ggccgctgcc ttggcccaca
     3661 cggactcggc tgcgagctac caggcccttt tggagaccct caacgacgcg aatgtgttcg
     3721 tgagaaatat tgccgaagtg acccttgaaa aacgccgtcg ccggatggaa tcgcaagaac
     3781 aaggtcagaa gaatgcccct tccggcgagt cacagccccg gcagcctcag ccatcgggga
     3841 tcatttcgga tgaaaccaac gttagccatt tggacacgtc acccacgaca acatcatgac
     3901 aagccccact atgcgacgat ttacccaggc attactcgca cgcgcaatct ggcgattggg
     3961 ccatgttgca ctggtccagg ctgtggcttg ggtaataacg acgtttttgg tctatgggtc
     4021 agaagcggct gcctatggcc aaggcagaac gccgctagaa atcctccacc gggagtttca
     4081 gcgaagagca acaggaagcg acaccgatca tacggcactc ttgtggggct tagcggtttt
     4141 tgtcggcctg gttgcagtgt tgatgattat cgcgtgggtc atcaactatt ttcaacaacg
     4201 tcggccgtac ttcagccaca cgttgctttt ccttgagctc tgttgtgtcc accgcatgag
     4261 gatcaaagac attctgttgc tctggaaatg gacatcacgg gaaaaaattc gtcccaggtc
     4321 gctgattttc gtcgatccgg ggctttggca aaacaagatc gaccacaatt tactcaacgt
     4381 caccgcggga tcaatcgccg cacaaacgac ctggaatcgt ctttacacac agttgtttgg
     4441 ggcctttgtt ccccagccca gtgagaattt ctcacccagg gaaaccgcga ggggacctac
     4501 aacttcttgc gaaaactgaa cgccgccccc gatgatttga tcggggtgac cgatcttcag
     4561 gtacgtaaag acaatgctgg ccgggtcaat aggcctttgt ctgaccggga atggcacacg
     4621 gatagatacc gtcagggccg ggcttgggtt gtgggacgct atcccacgta tagccggacg
     4681 gcgacaagtc gagttctgac ttgatagcct cgtcccaagt cacgatttgg cccgaatagg
     4741 tagccattcg tccaagaatc gccgtcatcg tccccaggat agttgcatcg acttcgttat
     4801 aggcctgccc cgccatgtgg gcttgcaaga agtcgttcca ttccaattgg tggccatctg
     4861 gtcctcgttc tacccgcacg ggatctttcc cctgcacatg gatgatagcg tggccgtgac
     4921 cttcgatact gacgtatccc ttcgttccat gggcgtgttc agaaaagcta tcccagcaac
     4981 cggggatatg gcggcagtag ctatacgccc gaacgccgtt ttcatagacg aattccacag
     5041 cgtggtgatc gtaaatctcg ccgtagtcct tgccgatgcg gaattggcga cctcctgtcc
     5101 cttgagccga gatgggatgc ttattgccca tcatccaatt gagaacatcc aggtcgtgga
     5161 cgtgctgttc cacgatatga tcgccactca accaggtgaa gtaataccaa ttgttcacct
     5221 gatactgcat ctcggtctgt tcgggcttgc ggggtcgaac ccagacaccc ctcgagttga
     5281 aatatgcccg catgtacatc agttccccaa tctcaccatc ccaaatccgc tggatcattt
     5341 ccttgtgttt aacttcatgc ctgagatgat gtcccacagc cacgcgaagc cctttcttct
     5401 tagcttcttc gttcgtagcc atgatccggc ggacaccggg ggcgtcaacc gccaggggtt
     5461 tctccataaa gacatgcttc ccggccttga cggccgcctc atagtgcatg gggcggaatc
     5521 ccggcgggct gcacaaaagg acgatgtcgg cgtcggtctc catgagtttc aaatagccat
     5581 ccagaccagt aaactgccgg tccggcggga catcggccat ctcgccgaag cgacctttca
     5641 gggcatgaag gctggcatcc acgcgttcct tgaaggcatc atgaacagcg accagcttga
     5701 ccttcgcccc gactttattc accatcgcat cgctgacagc cccagttccc cggccaccac
     5761 atccggaaag ggcaatcttg acgacgtcac tccccgcagc atgaacgaat cgcgtccaag
     5821 gccaagtcaa aacggcccct ccagccgccg tggcctgaag aaaatcccgg cgagttgtat
     5881 caccctggct ggcattttga ttcatgattg agtctcctca caaagcgagc gtatcacctc
     5941 tcgacccatg atttaccact tcgccaacag cgagcggaag tcaacaagct gcatgggcaa
     6001 caaacgaaga aactacttca cccacagacc gccgcccctt tgccgctaag tttcgtgcgc
     6061 ttacgttgta ccaacgactg agacgggaat caaacccccg gggaacgttt tggccggaac
     6121 agagaccgtt ccgaggaagg gtatcatcgt tggtggtcct tgcgtcacga cctcgcggca
     6181 tacgcgcaca actgggaaat gattctggaa aaggtgattc gcgtggcaag tgccgcaacc
     6241 gtccgacgct ctcaagcggc agcgcggtgg ctggggcttg tttgtgggcg tgacagtggc
     6301 agcgtggttc tacgcgtgtt ccgtcggtca tgccaccact gtttccaacg atgccaactc
     6361 gcatatggcg cgcagaatac cgacgaccat agaattgcca aaaggcgaag ggccggtgcg
     6421 gatgagagtg cccattcgca taatcgcttc aaatagtggt attcttcagg ggaatcaaaa
     6481 tcttccgtat agtcgtggat ctcacagaat tcgataggcg gacggcccga tggaggctcc
     6541 ctttgagcga gaaagtagaa ttcccatagc tttccgcggc cccctcttgt ggcatcgccg
     6601 ataacttgga tcaagcaatg ggtcacgaaa tcggaataat cccgcgtaaa ggacaaacca
     6661 aataattcgc cgtaacgttt gcctgtaaag atactggctc cctggcgccc gaagatcacc
     6721 aacccaccgc ttgccgcccg acatatggtg atcgcagcat ccgtgtataa aacgttgccc
     6781 aggaggacga ccgttctact tgaccatagg cacgccgtgc taaggaacgt ctcaacgacc
     6841 cagcggcgct gttccggaca aaatgagtca caacctggaa tgtgcaagcg cggatcatgc
     6901 gtcacaatga cgggcgagac tccgaagtag tgttgaacct gacctgcggt gcggacgagc
     6961 aagggaacgc caaaaatcgg ggcgagttgc ttaggtttgc ccagatactc gccccaccgt
     7021 tctccttcac cggcacagag gattaagacg cgcgtatcta gtggaatggc gttcggcaac
     7081 acgatcgttc catccttgcc ttcatttcca ttcggtatat caagggcaag cctctcataa
     7141 attttcggat gtaatgtggc tgagtagtgc gaaattgctt gttcaagggt actttgatta
     7201 ttgtcggcga tagataagat taatcgagat tcgtgtcaag ggcgaaatga atgtttttcc
     7261 gctccccttt tggttcccat catgtggggt gttagctgtc tgccagcgtt attcacccat
     7321 ttgatgcggg ggacgaatcg atccatccgc caccaagaac acgagtgccc tggtagcaga
     7381 ccgccgcctg accgggggcc actccgtagc acggtttgtg aaacctgacg agaaaccgat
     7441 ccgacgagag tatggtgacc gtagccggga cggccggggt tcgataacgg attttcacct
     7501 ggcaggggaa tggctcggcg ggaggatcaa tcagccagtt tgccccggag gctgtcaagt
     7561 cccatctggc caattcctcc cgtgttccca ccaccacccg gcgggtatcg gcttcgatgc
     7621 ggacgacata ccgtggctct ccgaaggcga tacccagccc ttttctttga ccgatggtaa
     7681 accgttccaa tccgccgtgg tggccaacga cctcgcctct tgtcgtgacg atttcacccg
     7741 acgtgtccac gttcggccga tactgtttga caaatcgggc atggtccttg tcgggaacga
     7801 agcaaatttc ttggctgtcc ggtttgttgg ccacacgaag gccgagttcg gtagcgatct
     7861 tccgaatctc ggccttggtg tagtttccga tggggaaaag cagtcggggc aggtcttctc
     7921 gccgcagccc aaataggaca tacgactgat ccttgctttg atcccgtcca cagaacaatc
     7981 cgtagttctt gtcctcggtg gggataatct gagcgtaatg gcccgttgcg atgaaatcgg
     8041 cctgcaaggc gtcggcatag tcgaagagct tgccgaattt taagcgatta ttacaaacaa
     8101 tgcagggatt aggcgttcgg cctcgcgtat attcatcgac aaagtaatgc acaatttgcc
     8161 gaaactcttc ctcaaagttc accacgtaga aaggaatccc gagccgatca gcgacacggc
     8221 gggcgtcttc ggcatcttct gcgctacaac aaccttgttt ccgcccgggg agcacgggaa
     8281 gtgtgggatt ccgcccctcg tttacgcaat gaagcgggct acgaatgcca tggcgcataa
     8341 agacgccgat gacctcgtgc ccctgctctt tgagaagatg ggccgagacg ctgctatcga
     8401 caccgcccga catcgcgacc aaaacccggg ccatcaagca ttttttcctg gagagagcct
     8461 tccaaaaatg cgattccaac ctcaccccaa tcctgtgaga aagacactcc atcccctact
     8521 gagattgtac gcgacgaagg taaaagacag ccaccgacac gaagcaacag accgccctta
     8581 caatttcggc aatgcggcgt caagggtagg acgcagacac actaccatgc cgagacgtgt
     8641 tagcggtgcc cccagctcac cctcaggcgg cggatcgttt tgctgtgtca gccgtttgat
     8701 cagataattt cagttgcgtc tcgcccgttg ccgaaagctt ccaggtcagt gtttcctgac
     8761 ccggatggag accgatgtca tcgacaactt taacgtcctg cccggcccca tcaaacaaaa
     8821 gacccatggg gaccttccac agcgtgagta gaatgatttt taggtccagt agcaggttaa
     8881 agtggtccac gtaatagacg tcgtaggcca atcgttggct gaacttaagt gcggcccgac
     8941 ctttgacttg ggccaggccg gtgattcctg gtcgcatctc atggcgtcgg gcttgttccg
     9001 gggtgtagcg ggggagatat tcgacgagga gcggtcgtgg cccgacaaag ctcatatcgc
     9061 ccttgaggac attccataac tggggaagtt catcgagact aaagcgccgc agccaacggc
     9121 ccagtggtgt gagacgctcg gcatcaggca gcggttgacc atccgccgac caggcgtttc
     9181 gcatggtccg aaacttataa aggacaaaaa gcctgcctcg gtagcccggc cgaacctggc
     9241 gaaaaatgat cggccggccc atggttatcc aaacggctag cgctgtggcg gccatcacgg
     9301 gtgaaaggat gatcaatgcc acggctgccc cgatgatgtc gattccgcgc ttaatcgcca
     9361 gttgcgtttt tcgccatgcg gtgaccattc cgcttattgt cctgtttttc tccaatttgt
     9421 tccgtttctc gcccagcgcc cgagaagatg ggcaccagtt gtctttcacc cagaacgccg
     9481 cactcaagcg gtttccccac cccgagcaaa gagtagcata agcttacgcc tggctccctt
     9541 tttcgatcaa gacagaattc gttggaccag tagtacgaaa acccggccaa tcccccactg
     9601 cccccatttt ccctggatat cgcactctca tcgtttgcca ttgacgattg tcgtgtgctt
     9661 taggtatatg ctttccccac tcaggcttgc ctctgagggc tggctttctg cgcgcttgcc
     9721 caggggagtt ggccaactcg ggaagacgac acgggcaatc ggaaaggtgg ttctggatgg
     9781 accggtgtgt cctccgccgc tgttggactg tgctgctgac tgccatcttg gcggtcacct
     9841 gtggctgcgc gctaagacac ggaggacatg tacgaccctg ggcgaaaccg ggactattcc
     9901 tgcgaccgca aatgctcagc cagtccccca cgtcactgac ttcgcggacc atccaatttc
     9961 tgcggcagca caatctcgat tcctccttgc atgaagaccc tcggacactc cttcagcagg
    10021 ttcaggcggt cattcaaaag gaaccacagg ccgataatgt ttacgcctat gctgagatta
    10081 gctatgtcct cgcaaaacgg gtggaacggt cccagccgcg gttggccctc gatttttacg
    10141 gcggcacggt tttaaaggcc taccagtacc tgttcgaccc caagtttgag gacacgcgaa
    10201 atccgtatga cccgcggttt cgtggggcgt gcgaccttta caattcggcc ttggaagccg
    10261 ccttacgcat cgttcgggcc aatgaatcac ttcagccggg aagtgctttt tccgttcgta
    10321 cggctgacgg ctattggaca ctcacctgta ccatctgcga tcgtcggtgg cgggccgaag
    10381 attttggaac atttgaattc gtttcggatt atcggatcac cgggctacga aatcaccacg
    10441 tccattatgg cctgggggtg cccatgatcg ccgtgcggaa gaactatccg ggcgagccgc
    10501 ccgccgctcg ctactatccc cccgacttga gttttcctgt gacggtgttc ctccgaccag
    10561 agattccctc tgccacagag tcccaagagg aagaccgtca ttacgaaggc cggttggagc
    10621 tttatgaccc cgtggccacg atgaacattc aggttggaaa ctatgttgtt cccctccagg
    10681 ccgacctgac cacacccctg gccacgttcc tgtctaatcc ggccttggga tcgtctctgg
    10741 ccacggtcgg gctactgcgg ccggaaaaac tgttgactct ccaaccgggt ctgaacaaac
    10801 ccttaaccgg tctctacatg gttcagccat acgagccgga taagatcccc gtcatcatga
    10861 ttcacggctg gtggtcaagc cccatgacgt ggatccagat gttcaacgat ctccgtagcg
    10921 atccgctgat ccggcgacgt taccagtttt ggttctatct ctatcccaca ggtcagccat
    10981 tttggatttc cgccgcacag tttcgccgcg acttgcggga attacgtcag acgctcgatc
    11041 ctgaccatcg gcaaccggcc ctcgaccaaa tcgtcctgat tggccacagc atgggagggc
    11101 ttatcgccga actacagacc attgatagtg gccaagactt ctggaaattg gtgagcgaac
    11161 atccttttga ggaaattcgc acggacccta tttcgcgtga ggaattgcgc gaaatcttct
    11221 tcttccagcc caatccgtcg atacggcgga tcatcaccat cgccacaccg cacttgggat
    11281 cacatttttc taatcaaatg acccagcggc tagctgcccg cttgataagt gtgccgaaag
    11341 aacttctcca gtcccagact caatttttcc gggaaaatca agaccttctc cttcctaaca
    11401 acattctcca gatcaccgac agcgtggatg ccctcgatcc cgattgcccc atctttgctc
    11461 tgatgaacca agctcggcgg gcaagctgga tcgtgcacca caacattgtg ggggcgtcgc
    11521 tggaagatcg tctctggggc cgaatcacgg gtaccagcga cggcgtcatc tcctgggaga
    11581 gtgctcattc cgataaagcc gattcagaga ttgccgtccc cgccgagcat accagcgttc
    11641 agtcacatcc attgacgatc cttgaggtca aaagaattct tttagaacat ctggccgagc
    11701 ttcaccaaga acactccctg ttcacgagtg gtgcgctgcc tgccgacgtg gccgtaccgt
    11761 ctcctgtccg gctgccacca gtggaatatc cctaactccg ccccatgatt ttgcgcattc
    11821 tgcctatact gaaaggatac gcttccgtcg tttctaattc ccgtgttacg gagtccatac
    11881 cctatgtcta tgacctaccg gattgagacc gattcgatgg gcgaagtccg actgcctgcc
    11941 aatgcctatt atggggcgca aactcaacgg gctgtggaaa actttcccat ctcgggacag
    12001 actctgcccc cagaattgat ccacgccctg ggactggtta aatgggcagc agcccaggcc
    12061 aactacgatt tgggcctgct tacccgggga ccacgggccc tcaatcccgc gcaggtccaa
    12121 gccttgctgc aggcctgtca ggaggtggcc gaggcacgct tcgatgacca gtttcctgtc
    12181 gatgtgtatc agactgggtc gggaacttcc agcaacatga acgtcaacga agtcatcgcc
    12241 aatagagcca tcgagctttc cggcggtgat cgctttcaat ccgaaaaacc gatccaccct
    12301 aacgatcacg ttaatatggg acagagcacc aacgacatgt tccccaccgc catccatgtc
    12361 gcactggccg tggcgataca tcgagacctg atcccggccc tcaaggaggc ccggagcatc
    12421 cttgaggaaa aggcggagcg gtggggcgac atcatcaaga ttggccgcac ccatcttgcc
    12481 gacgccacgc ccttgacgct cggccaggaa atgagtggcc ttgcccgcat gatggagctg
    12541 gccgctgagc gggctcaacg tggagtggat gcccttatgg aactgccggc cggcggaacg
    12601 gcggtgggga cgggcatcaa tactcaccct gactttggac gccgggtcac cgcgttcctg
    12661 gcggaaaaga cgggacttcc tttccgcgag gcggtcaatc atttcgaggc gaacgcccag
    12721 cgcgacggcc tcgtcgaatg ccacggccaa atccgggccg ttgccgtgag cgtcttcaat
    12781 gtagccaaca atattcgctg gctctcctcc ggaccgcgct gcggctttta tgagatcatc
    12841 cttccggagc ttcagcctgg gagctccatc atgccgggta aggtcaaccc agtcctctgc
    12901 gaaagtctca tgcagatcgc cgcgcgggtc atcggcaacg atcaaaccat tgcctttgcg
    12961 ggggctagtg gcgggcagtt ccaactgaat atcatgatgc cggtgatggc ccatgtggcc
    13021 ctcgaatccg tacgcctcct cgcccggggt gtgcggatct ttgcagaaaa gaccttgcgg
    13081 gggatgcagg ccaaccgcga agcctgcgag gcggccgtgg aaaagagtct ggccctggcc
    13141 acaagcctta accccattgt cggttaccaa aaggccgcgg ccatcgccaa agaggcattc
    13201 aaaaccggaa aaactatccg tcaaatctgt tcagagcaaa aaatcttatc cccagaacag
    13261 cttgccgagg tcctggaccc actgaagatg atccgaccac gcggcgacga tccttcgtca
    13321 ccagcgtgac cttctgctga agagccggtg gatctccttg ccttttcgca acggccgatc
    13381 actagggtct ccgttcatcc caacgaatcg ctcggcccgt ctgcgtctct tccagagacc
    13441 agttacagga gcccgctggt ccgtggccaa gaatagtgct ctcacttgag agttctctct
    13501 cagaaatcat aacaataaat ggtcacaagt agttaccttg tcgtgagctt ataccgttga
    13561 caagatagtt ccgccaagta aaatgtggga agtgttggcg ccaaatcagt ctggcg
//
LOCUS       DAOSUT010000112        13597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2710_length_13597_cov_9.012037, whole genome shotgun sequence.
ACCESSION   DAOSUT010000112 DAOSUT010000000
VERSION     DAOSUT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_13597_cov_9.012037"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLD05_10770"
     CDS             <1..420
                     /locus_tag="PLD05_10770"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77966.1"
                     /translation="GMFRLAQTNDYHFVTTFSEIIDGTSNVIAVGEVSESPNISRTQT
                     DRMFPLWAGGNNDWAGQWRISSWARVAGPYAVINCKDKMPIGDVTLADYAFGSKHPGG
                     ANFLLGDGSVRFLSETIDGTLYGRLADIRDGNPVTMP"
     gene            591..1046
                     /locus_tag="PLD05_10775"
     CDS             591..1046
                     /locus_tag="PLD05_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77967.1"
                     /translation="MSLAMQCLRVFLVLAVAGVLATAIGCSKSGAAYRTAPVKGKVIY
                     NGQPVTSGGIRLQPIAVPGASESNPGKPAGGEVRSDGTFVLSTYKDGDGAVVGRHKVS
                     YIPFSKGAETYEDKPEPSPYLGLVPKEQEVEIKPGQNELTIELVPMTQR"
     assembly_gap    1177..1276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1606..3018)
                     /gene="glnA"
                     /locus_tag="PLD05_10780"
     CDS             complement(1606..3018)
                     /gene="glnA"
                     /locus_tag="PLD05_10780"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOP77968.1"
                     /translation="MTPKEVLAFCRERGVKSVDLRFMDFPGLWQHLTIPVTALTEELF
                     EDGIGFDGSSIRGWQRINESDMLLIPQPETAALDPFAEIPTLMMICNIQDPVTKEDYT
                     RDPRNVARKAVNYLKSTGIADTCYVGPELEFFIFDDIRFDQSANYGFYYIDSIEGQWN
                     RGRDEKPNLGNKLRYKEGYFPVPPADQLFNIRNEMMHLMIECGLPVEAQHHEVATGGQ
                     AEIDLRFDELVTMADRVMIYKYIVKNVARKHNKVAVFMPKPLFGDNGSGMHTHLSLWK
                     KDQPLFAGSGYAGLSDVALYAIGGLLKHAPALCAFANPTTNSYKRLVPGFEAPVNLAY
                     SQRNRSAAIRIPMYSASPKAKRLEFRCPDPSCNPYLAFAAMLMAMIDGIQNKIHPGAP
                     LDKDIYDLPPEELKEIPKTPGSLEEALAALRNDHEFLLRGDVFTPDVIDTWIWYKTEN
                     EVDAMKLRPHPYEFCLYADI"
     gene            complement(3321..4019)
                     /locus_tag="PLD05_10785"
     CDS             complement(3321..4019)
                     /locus_tag="PLD05_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77969.1"
                     /translation="MEHSLHDQLKRFYAGPNSSIEVVIDGFRVDALSHGWLIEIQQGP
                     LVAIRKKIQTLMQRYPLLVVKPVVVEKQIVNLSRRGGPVVSRRRSPKRGQSWDIFDDL
                     VHMTKIFPHPNLRIDLALVDIEEIRYPGPRQRWGHDGYRLADRRLLRLRETMVLNTAG
                     DLRRLLPALPEPFESRDLAEILKIPRWVAQRIAYTLRESGAVQTVLRDRRGHHYRWVD
                     TPTDADDRLALLTA"
     gene            complement(4204..4761)
                     /gene="pyrE"
                     /locus_tag="PLD05_10790"
     CDS             complement(4204..4761)
                     /gene="pyrE"
                     /locus_tag="PLD05_10790"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOP77970.1"
                     /translation="MFDRERLRDLILQKALHFGQFTLASGKTSTYYLDCRKITLDPEG
                     ARLVGEGILDILLQSSFPDAVGGMAIGADPITAAVVTMSAVRGTPIRGFMVRKEPKGH
                     GTQQYIEGPVEPGDRVAILEDVLTTGGSALKAIERAVAFGLHVTQVIGIIDRLEGAGQ
                     AFAERGYPFAALFTIRDFGIEPNPA"
     gene            complement(4902..6197)
                     /locus_tag="PLD05_10795"
     CDS             complement(4902..6197)
                     /locus_tag="PLD05_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOP77971.1"
                     /translation="METLKTIGVLVVLLVVGYLGYKAVTNPPVTPPNETPTSQSPTVA
                     DATNVNIPIPELPPGMENQSADRSAKTSSLVSSPIVPPASSGGQVPTASLTARPNSSS
                     QEVAAQISNSGDLEGGLSSGQKSPDLSVIPPQPVVGSSFSARPSTEGAVPPIASPMGD
                     VRSGALATSGPAPEAESVSSQVMSQNAVTPPSSSSAVRPEFANFLEVACQKLDRGEFA
                     QVHQVLSAWYADPRLTPEEDAQLTDLLDQVAGTVVYSRQHILEPPYTVQPGDTLPQIA
                     ERFAVPWELLAKINGITQPDQLSPGMQLKVVRGPFEAVIRLQRRELVLMVQGRYAGRF
                     SIGLGRDAKGCEGVYQVREKTLNPRYYSDQGVIEANDPRNPLGAKWLGLEGRLGIHAA
                     RDPRSFQDPEAPGAIFLSPQDMDDVFDILDVGSRVVITP"
     gene            complement(6720..6869)
                     /locus_tag="PLD05_10800"
     CDS             complement(6720..6869)
                     /locus_tag="PLD05_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOP77972.1"
                     /translation="MMQEVITAVVFAVFSTLYMRQPVNWNYAIAGLCLVVAAFFMFRG
                     ITHGA"
     gene            7119..8138
                     /locus_tag="PLD05_10805"
     CDS             7119..8138
                     /locus_tag="PLD05_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP77973.1"
                     /translation="MVTQNRPQVLLSGFADESANHKTAIEQFVAFAALGLSYYSIRFI
                     DVGQGVKNVMKITPEELDKVIQLQQEYGLKVSSLGSPIGKIKLKDVEDGTKNVYIPFE
                     RYLQEDVARACELAQALGTKLIRGFSFYHPKGEDPRPYVTEVVDRLGQIADKCQQAGL
                     IFGLEVEANLVGQNGFLLAEIYDKLKHPALVLVFDGANLVVQGYTPGEIFEQYQAMKP
                     GLGWMHIKDYRRPQRDVKGGHVDEDMLKNFVPANMGDSGHEVILRDFATILPELEQKL
                     QAQGVPGVFLDLEPHVKGGGQFGGFSGPDGMGVALRGLCRLLDYVGISYHLRDFDDVR
                     AARGF"
     gene            complement(8319..9074)
                     /locus_tag="PLD05_10810"
     CDS             complement(8319..9074)
                     /locus_tag="PLD05_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77974.1"
                     /translation="MLRTLCWVMVVGVTMGTASLLAQDAILNHFYGQGVHKFFARDYV
                     GAFEDFNTAIENGSDDPRCFYYRGLTYLHLGREEEAKSDFEKGAQLEMQAARKFFDVS
                     KALERIQGRQRVMIEQYRTKARLAAMVELERQRKARYEELRREEARVLDRAPPAVGIP
                     EAAPAPPSAPPAAPPAAPSEPAVAEQPPAAQPPAPAAPPAAPPAETNPFATEPSAPAQ
                     PAMPPAPEQEPAAPPAPPAPPQPPAPAENPFAT"
     gene            9416..10036
                     /gene="rdgB"
                     /locus_tag="PLD05_10815"
     CDS             9416..10036
                     /gene="rdgB"
                     /locus_tag="PLD05_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOP77975.1"
                     /translation="MEKPIGVIGTGNHAKLIELADLLTPAGVTWKFLKDFPHVGEVEE
                     TGCTFSENARLKAVTYAVRLGEWVLAEDAGLVVDALGGQPGIHSARFAGEPRDDARNI
                     QKLLELMKDIPPERRSARFVCYMALADPRGEIQAEAEGHCCGRITFEPRGTQGFGYDP
                     VFEIPEYHRTLAELGLAVKGCLSHRARAARAILPQIIRLVDSGKWR"
     gene            10130..12004
                     /locus_tag="PLD05_10820"
     CDS             10130..12004
                     /locus_tag="PLD05_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOP77976.1"
                     /translation="MSLRWLAGGTLLVVFCLGFVWAFWFPHEPPADLTFCNLTEIKTI
                     DPALVTGQPEGRVVWALFEGLTTYDPKTLAPQPGVAESWTISQDKRTYTFHLRKEARW
                     SDGKPLTAEDFVWSFRRLLHPETASEYAYELWHVVNAEKYTTGRVTPGDPVEIELLEK
                     PPGAPPFASGILLRGRLVGIEPANSALGGQTTGEPVYLVEIDGRIERFQKPSREGLPP
                     PDTAARPYRWILYDFEHVGIKALDRHTLQMTLTHPVPYFLEIIGFFPMMPVPRHCIEQ
                     YGYPNWTKPENLVCNGPFILKYRRIRDRIRLVKNLHYWDRENVHLNIVDVLAAESAIT
                     GLNLYMTGAADWIPVVPNEVVSELLRQKRPDFQPSPYLAVEYYVVNTQRPPLNDPRVR
                     RALALAINKRELIDRILQTGQQPALSFVPPSISDYIAYQPPQMPDYDPETARQLLAAA
                     GYPNGRNFPKMEILYNTLESHQAIAELIQAQWKRTLGIDVRLQNQDWARYLASRRQGE
                     FWIARAGWIADYLDPNTFLEMMTTDNPNNHGRWSQPEYDRLVALAQVEPDETRRFAYF
                     RQAEEILLQEMPVLPLYFAVTRAMVRPYVRGYYHNVLDIHPLKGMSIDHAQKASLLGR
                     "
     gene            12095..>13597
                     /gene="crtI"
                     /locus_tag="PLD05_10825"
     CDS             12095..>13597
                     /gene="crtI"
                     /locus_tag="PLD05_10825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOP77977.1"
                     /translation="MEVNLWLTTFLAGRRSKLGDPYGLHQVVIIGAGPGGLGAALLLA
                     KAGLDVTVLERLSVIGGRCSPITINGYRFDRGPTFLHYPCALKSIFRAVGKELEEEVP
                     MVRLDPMYRLFLGERGYLDATADIAQMEAHIAKFQPRDCKGFEQFLKDNDGKFQRVIR
                     TLQRPFDGLRDLFSSEILNLLPVLRPFTSLQQDMRKFFSDPDLQVAFTFQSKYLGMAP
                     DRCPSMFSILSYLEYAYGIFHPIGGCNAVPQKLAKLSKELGAKFHLNQEVQEIRFEGR
                     RPVAVKTQDAEFPCDLLVINADFARAMERLVPNVLRRRWSDEKLRSKKYSCSTFMLYL
                     GIEGQVENLLHHNIYIPPHYREYLDDVDVRHVLSDAPAFYVQNATLTDPTLAPPGHST
                     LYILVPVTHQHPNVNWDQERARYREVVLDNLTRIGIPDLRSRIRAELVWTPADWEAWQ
                     IYRGATFNLAHSLDQMLIFRPHNRFEELERTYLVGGGTHPGSGLPTIFESA"
BASE COUNT         3018 a         3724 c         3720 g         3035 t
ORIGIN      
        1 gggatgttcc gattggccca gacaaatgac taccatttcg tgacaacctt ctcagagatc
       61 attgacggta cgagcaacgt catcgccgtt ggtgaggttt ctgaaagccc caatatctcg
      121 cgaacccaga cggaccgcat gtttcccctt tgggctggtg gcaacaatga ctgggctggc
      181 cagtggcgaa ttagctcttg ggcacgcgtt gctggtcctt acgcagtcat taactgcaaa
      241 gataagatgc ccattggcga tgtgaccctg gccgattacg ctttcgggag caagcacccg
      301 ggcggtgcga acttcctgct cggcgatggc tctgtccggt tcctgtctga aacgattgac
      361 ggaaccctct atggccggct agccgatatc cgggatggca atcccgtaac catgccgtag
      421 tttgcatagg ggattacaag tcgccaaaac cgtaatccta agtttggcga tcacgggtgg
      481 ttaataaggc ggctgtcgtt ggcaacggta gccgccttgt atcataataa tttggtgtct
      541 gtggcggcgt tcgtgcgcgg gatgtgatgt cgatttcagg aggcgtccct atgagtctag
      601 caatgcaatg tttgcgtgtg tttctcgttc tggctgtggc gggagtgctt gctacggcaa
      661 tcggttgttc caagagcggg gcagcctaca ggacagctcc cgtcaaaggg aaagtcattt
      721 ataacggtca gccggtgact agtggcggga ttcgtctgca gcccatcgcc gtcccaggtg
      781 cttcggaaag taaccccggc aaaccggccg gtggagaggt gcggagtgac ggaacgttcg
      841 tcttgtcaac ttacaaggat ggcgacgggg ccgtcgtcgg caggcataaa gtctcctaca
      901 tcccgttctc caaaggcgcg gagacgtacg aagacaagcc ggagccgtcc ccttaccttg
      961 gtttggtccc caaagaacaa gaggttgaaa tcaaaccagg ccaaaatgag ctcaccattg
     1021 agctggtgcc gatgacgcaa cgttgaaatg tgttaagcaa ataacgtttt tcacgagacg
     1081 attgacccgt caaagctatc ctatcgctca ctgacggttc gcctgtgact cgcaagaaag
     1141 cccacctgac cgccgcgacg tggatcgcgg cttattnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnaaaa agagcagccg gtccaaaaga atccggggag gcacttgatt
     1321 ctttggaccg gcttgcttgg gcggagaggg gtggtgcgat ggatgttcca tcacccttat
     1381 acgtattata cctcatcttt tcgcaaatgc gattccctct cctaaggtct atctcaaggc
     1441 tccctctccc tctgggagag ggttgggtga gggcaagcga tcgtcggagt cgatcgagaa
     1501 aaccggcggc aatccgagcc accctcaccc ccggcccctc tcccgctgaa cgggaaaggg
     1561 ggaggttgtg ggatcggctc taaattcatt ttcccatcct acgtgtcaga tgtcggcata
     1621 aagacagaac tcgtagggat ggggacgcag cttcatggca tcgacctcgt tttccgtttt
     1681 gtaccagatc cacgtgtcaa tgacatctgg cgtaaagaca tcaccccgca ggagaaactc
     1741 atggtcgttc cgcaaagccg ccaaggcctc ttccagcgaa cccggcgtct ttgggatttc
     1801 cttaagttcc tcaggtggca ggtcgtaaat atccttatcg agcggtgcgc ccggatgaat
     1861 cttgttctgg atcccatcga tcatggccat cagcatggct gcgaaggcca gataaggatt
     1921 acaggaggga tccggacagc ggaactccaa ccgtttcgcc ttagggctgg cactgtacat
     1981 ggggatgcgg atggctgccg accgattccg ctgtgagtac gcgagattga caggagcctc
     2041 gaatcccggc accagtcgtt tgtagctgtt cgtcgttggg tttgcgaacg cgcaaagggc
     2101 tggtgcatgc ttcagcaggc cgccgatggc atacagagcc acgtccgaaa gaccggcgta
     2161 gccactccca gcaaaaaggg gctgatcttt tttccacagc gacaaatggg tatgcattcc
     2221 cgagccattg tcgccaaaca gcggtttcgg cataaacacg gccaccttgt tatgcttacg
     2281 ggcgacgttt ttcacgatgt acttgtaaat catgacgcgg tcggccatcg tgactaactc
     2341 gtcaaagcgg aggtcgatct cggcttgccc gccggtagcc acctcgtggt gctgggcctc
     2401 gaccggcagc ccgcactcga tcatcaaatg catcatttca ttccggatat tgaacaactg
     2461 atcggcgggc ggcaccggga aatagccctc cttatatcgg agcttgtttc ccaagttggg
     2521 cttctcatcg cgtccccgat tccactgtcc ctcgatgcta tcgatatagt agaagccata
     2581 attcgccgat tggtcgaacc ggatatcatc gaagatgaag aactccaatt ccggcccaac
     2641 gtagcaggtg tccgcaatgc ccgtgctctt gagatagttg acggcctttc gcgcaacgtt
     2701 gcgcgggtca cgtgtatagt cctcttttgt aacggggtct tggatgttac agatcatcat
     2761 tagggtcggg atttccgcaa agggatccag ggcagccgtc tccggctgcg gaatgagaag
     2821 catgtcgctc tcgttgatcc gttgccatcc tcggatactt gacccgtcaa agccaattcc
     2881 atcttcaaac agttcttccg tcagcgctgt gacggggatg gtcagatgct gccagagacc
     2941 tggaaaatcc ataaaccgca ggtcaaccga cttaacaccg cgctctcgac aaaacgccaa
     3001 aacttctttg ggggtcatgt gtcactcccg tcgtcatgcc taccaggaaa tttgctcatt
     3061 gcagcgtgtc tccgcgccgc tctccgccga gcaacacatt caccacgttg caatggccgc
     3121 gttcttcaag caaacaccgt gccaatgcca gaactcggcc cacatactgt cttttttacc
     3181 ggttttgtcg atgaagagta gcccgtttcc caacaacgat tcacgcaatg agattcttcg
     3241 caccatcaac cggtgattaa ctcttgcgca gccctgcctc tttcctgggc agatgatcga
     3301 tctcatgagt tatcgacgtt ttaggcagtc aaaagtgcca accgatcgtc ggcgtcggtc
     3361 ggggtatcaa cccagcgata atggtgacca cgccggtcgc gaagaactgt ttgaacggcc
     3421 cctgattcgc ggagggtgta ggcgattcgt tgggcaaccc agcgaggaat cttaagaatt
     3481 tccgccaaat ctctggactc aaaaggttct ggcaatgccg gaagaagtcg ccgcagatcg
     3541 ccggccgtat tgaggaccat tgtctcccgc aagcggagga gtcttctgtc tgccagccgg
     3601 taaccatcgt gtccccagcg ctgcctcgga cccggatagc ggatttcttc gatatctacc
     3661 agggccaaat cgatcctcag gttgggatgg gggaaaatct tggtcatgtg gaccaaatcg
     3721 tcgaagatat cccatgactg accacgtttg ggacttcgcc gccgtgaaac gaccgggcca
     3781 ccccgccggg atagattcac aatctgtttt tctaccacca caggcttgac gaccaatagg
     3841 ggatacctct gcatgagtgt ctggattttt ttgcgaattg ccacaagcgg cccttgctgg
     3901 atctcgatga gccatccgtg gctaagagca tccacacgga acccgtcgat aaccacctca
     3961 atagaggaat ttggccccgc gtagaaccgc ttcaactggt catgcaatga gtgttccata
     4021 aaacgtccct ccgtgaaaga gccttgtcaa ttgagcaacc gcagttttcg acttttcgca
     4081 acctaccgat ggtgcggttt cgaagctgct caactcagga tgtccgcggg tcttgcaaaa
     4141 tcgacacgtt ttgcaaaaaa gtcgcacaga tgcatgtcaa agaagaaaat gatagccttg
     4201 gccttaagcc ggattcggct cgataccaaa gtcacggatc gtgaacagag ctgcgaaagg
     4261 atagccgcgc tcggcaaagg cctgacccgc accctccagg cgatcaataa taccgatcac
     4321 ctgtgtcaca tgcaaaccga atgcgacggc ccgttcgatc gccttcagcg ccgatcctcc
     4381 cgtcgttagg acatcctcca ggatggccac gcggtcccct ggttcaactg gtccttcgat
     4441 atactgctgg gttccgtgtc ctttcggttc cttacgcacc ataaaacccc gaatcggagt
     4501 cccgcggacc gccgacatgg taaccactgc cgccgtgatg ggatccgccc cgattgccat
     4561 cccgcccaca gcatccggaa aagaactttg aagcaggata tcaagaatgc cttcgcccac
     4621 aagtcgggca ccctcgggat cgagtgtgat cttgcggcaa tcgagataat aggtggacgt
     4681 ttttccagag gccaacgtaa actggccaaa gtgcaatgcc ttctggagga taaggtctcg
     4741 caatcgctcg cgatcaaaca tgcacagatt ccccctgacg attcccaata tttacgtcgc
     4801 gtatccgtat gattgtgact cgttagcagg atcgcgcgaa gccgggactg agcaaagact
     4861 gatcaaatca catgcgcctc atattcgcgc atcaacatac gctagggtgt gatgaccacc
     4921 cgagacccaa cgtccaaaat gtcgaagaca tcatccatat cctggggaga caggaaaatt
     4981 gctccagggg cttcggggtc ttggaacgat cgcggatcgc gggcggcgtg gattccgaga
     5041 cgtccctcca gtcccaacca tttcgctccc aacggatttc gaggatcgtt ggcctcgatg
     5101 accccttgat cactgtaata tctcggattc aaggttttct cacgtacctg atacacacct
     5161 tcgcaaccct tggcgtcgcg ccccagaccg atcgaaaacc gcccggcgta ccggccctgg
     5221 accatcaaca ccagttcacg ccgttggagc cggatgacag cctcaaacgg accgcgaact
     5281 accttgagtt gcatccccgg tgacaactgg tcgggctggg taatcccatt gatcttggcg
     5341 agaagctccc aaggaacggc aaatcgctcg gcaatttgcg gtagggtatc accgggctgg
     5401 acggtgtaag ggggttcaag aatgtgctgc cgcgagtaaa cgaccgtccc tgcaacttga
     5461 tccagcagat cggtcaattg ggcatcttcc tcgggggtta atcggggatc ggcataccag
     5521 gctgacaaaa cctgatgcac ctgggcaaat tcgccacgat ccagtttttg gcaggccact
     5581 tcaaggaagt tggcaaactc tgggcggact gcagaagatg aagacggcgg tgtaacagca
     5641 ttttgcgaca tcacttggga ggatacgctt tcggcttccg gagcgggtcc cgaggtggcg
     5701 agggccccac tgcgaacatc acccatagga gaggcaattg gcggaacggc cccttccgtt
     5761 gaaggcctag cactaaatga actaccgaca accggctgcg gtgggatgac gctcagatcc
     5821 ggagactttt ggccggagga caaccctcct tccaaatcac cgctgttgga tatctgagcc
     5881 gccacctcct gggacgagga attcggcctg gccgtcagcg aggccgtcgg gacttggccg
     5941 ccactgctgg caggtggaac gatcggcgaa ctgaccaggg acgaggtctt tgcggaccgg
     6001 tccgcacttt ggttttccat gcccggtggg agttcgggaa tggggatgtt cacattggta
     6061 gcatccgcca ccgtgggcga ctgagaagtg ggggtctcgt taggcggcgt tactggtgga
     6121 tttgtgacgg ccttatagcc aaggtaaccc accaccagga gaaccaccag cacaccaatg
     6181 gttttaagcg tctccatacc tgtcctccct gacgccacag agggccacat tcaaacccac
     6241 actgtggctc ggccattgag tcactcactg gtcagcgcgg cctcaattgc ggccggacgt
     6301 gcggctatcg taattacccg gagtccttcc atcctagagg atgggactca tgataagcag
     6361 aatagagatt ttctccaaga gcaattgcgc gcagcccaat ggcaagaata acccccgttg
     6421 gtggtgcttt gccagccggc atatctacaa ttgaccaaat ctatgtcgag aggaaggaaa
     6481 cggactccgg ggatagtcct aataagagcc tatcccataa tctcttttca cccgttaggc
     6541 aggagagatg ttgggggtga gggtgactcg aatcgcttcc ggttttctcg atcggctccg
     6601 acgatcgctc gccctcaccc cccaccctct cccggaggga gagggagact ttttgggata
     6661 agctataagc ataggcgtgc gtcatttggc cccgtttatt aaggaggctt tgatgtcggt
     6721 catgctccgt gggtaattcc tcggaacatg aaaaaggccg ccaccaccag gcataatccc
     6781 gcgatggcgt aattccagtt aaccggctgc ctcatataaa gggtggaaaa aaccgcgaac
     6841 acgaccgcgg tgatcacctc ttgcatcacc tttgcctcac gtctcgttgg attgagagga
     6901 acagccgccg cggttgccca ctgtttgaat ttggcaaagg cgcacaactg accatggata
     6961 acagaaaacg gggggttgtg aatccaggca aaacaaccca caatactggg gacttatccg
     7021 gcaaaggaag tgttttccgt gggaaacgca gaatgccggg gctggacgtt gactgcctta
     7081 tattggccac ccagaaacga actgatgagg tgcgtgaaat ggtgacccaa aatcgtcccc
     7141 aggtgctact ttcgggattt gcagacgaat ctgccaacca caagaccgcc atcgagcagt
     7201 ttgttgcatt cgcggcgtta ggactctctt attacagtat ccgcttcatt gacgtgggtc
     7261 agggtgtcaa gaacgtcatg aagattactc ccgaggaact cgacaaggtc atccaactgc
     7321 agcaagagta cgggttgaag gtctcatcgc tcggttcacc catcggcaag attaagctga
     7381 aggatgtcga ggacgggacg aaaaacgtct atatcccgtt cgagcggtat ctccaggagg
     7441 acgtggcgcg ggcatgcgag ttagcccagg ctttggggac aaagctcatc cgcggctttt
     7501 ccttttatca tcctaaaggg gaagaccctc gcccctacgt cacggaggtg gtcgatcgtt
     7561 tggggcaaat tgccgacaag tgccagcagg ccggtcttat ctttggcctg gaagtggagg
     7621 ccaatttggt gggacaaaac ggctttctcc tggctgagat ctacgacaag ctcaagcacc
     7681 ccgcgctggt tttggttttt gatggtgcca acctggtggt ccagggttac accccgggag
     7741 agatttttga gcaataccag gcgatgaagc ccggtctcgg gtggatgcac attaaagatt
     7801 accgccgtcc ccaaagggat gtcaaaggcg gccatgttga cgaagacatg ctgaagaact
     7861 ttgtaccggc caacatgggt gattccggcc atgaggtcat tcttcgcgac tttgccacga
     7921 tccttcctga actagagcag aaactgcaag cccagggtgt accgggggtc ttcctcgacc
     7981 tggaaccgca cgtcaaaggc ggcggtcagt tcggcggatt tagcggacct gacggaatgg
     8041 gcgtggctct ccggggtctg tgccggcttc tggactatgt gggcatatcc tatcatttgc
     8101 gggatttcga tgatgtccga gccgccaggg gattttgaag ttttcgtcct gcattgtccg
     8161 caggtggagg tttccgtgcg aaaaccctag ctccctggaa gtagccccaa aaaagaaacc
     8221 gcagcctgcg aaggctgcgg ttatttttac gactcatgcc gtgagtgact tgtttggcct
     8281 cgcggttgcc cttggggccc tatctcaaca cgctaaggtc aggtggcaaa ggggttctcc
     8341 gcgggggctg ggggttgagg tggtgcaggc ggtgctggag gagcggccgg ttcttgctcc
     8401 ggcgcaggag gcattgccgg ttgggcagga gcagaaggct ccgtggcaaa aggattcgtc
     8461 tcggccggtg gtgcagcagg tggcgccgca ggagccggcg gttgagccgc aggcggttgt
     8521 tccgctaccg ctggttcaga tggtgctgcg gggggagcgg ccggtggggc agacggcggg
     8581 gctggagcag cttccggaat gcccacagcc ggtggcgccc gatctaaaac gcgggcctct
     8641 tcacggcgaa gctcctcata ccgggccttg cgctgacgtt ccaattccac catagcggcc
     8701 aaccgcgcct ttgtgcggta ttgttcgatc atcactcgtt gacgcccttg aatccgctcc
     8761 agggccttgc tgacatcgaa gaacttcctt gctgcctgca tctccaactg agcgcctttt
     8821 tcaaagtcac tcttggcttc ctcctcccgt ccaagatgga gatacgtcaa accccggtaa
     8881 tagaaacacc gcggatcatc gctcccattc tcaattgctg tgttgaaatc ctcgaaggcg
     8941 cctacataat cgcgggcgaa aaatttatga acgccctgac cgtaaaagtg gttgagaatg
     9001 gcgtcctgtg ccaagagcga ggcagttccc atagtgacgc cgacaaccat tacccagcaa
     9061 agtgtccgca acatggagtc agctcccagc gactgtcata accttttgcg tgacccaagt
     9121 gcaatcgaac ctccgtgtgc gacagcctcc gccgtatcgt ttattctctt tcctgccaca
     9181 ggccggttat gggcactccg cgcaccattg agccagcctg ttacgttggc acggaaaaga
     9241 cctaccttaa cctttactct tgcccatact aattgggaaa accggcaatt tcaagctgat
     9301 gccataggcc gcgttaattt agcaaaacag ggaatgcgat taaaataatt agacttcgcg
     9361 ggaattgcaa gtggcccggt accaaaaaac ggatggctgt gacaaggaac cacgcatgga
     9421 aaaaccaata ggggtcatcg gaaccggaaa ccatgccaaa ctgatcgaac tagctgattt
     9481 gctcaccccg gctggagtga cctggaagtt cctgaaggat tttccccatg taggcgaggt
     9541 tgaagaaact gggtgtacat tttccgaaaa tgcccggctt aaagcggtga cgtacgccgt
     9601 gcgtctggga gagtgggttt tggcggagga cgcaggcctg gtcgtggacg ccctcggtgg
     9661 ccaaccgggg attcattccg cccgctttgc cggcgaaccc cgcgatgatg cccgcaatat
     9721 ccaaaagttg ctggaactca tgaaagacat tcccccggaa aggcggtccg cccggttcgt
     9781 ttgctacatg gccctggccg atccgcgggg ggagattcag gccgaggccg aaggacactg
     9841 ttgcggccgg atcacattcg aaccgcgcgg aactcaggga tttggctacg accccgtttt
     9901 tgaaatacct gagtatcacc gcacactggc agaattgggg ctggcggtaa aggggtgtct
     9961 cagtcatcgg gcgcgagccg ccagggcaat tctcccgcaa attatccgat tggtcgattc
    10021 tggaaaatgg agataatggc acgtttttca caggtttcga gctaacattg ggtccgtact
    10081 ttagtctctc attgcatcgt tgtttgggtc cttagaaaag gctggtggaa tgtcgcttcg
    10141 ttggctggcc ggaggcacac tactagttgt cttttgcctg gggttcgtgt gggccttttg
    10201 gtttccgcac gagccaccgg ccgatctgac gttctgcaac cttacggaaa tcaaaacgat
    10261 tgatccggcc ttggtaaccg gccaaccgga aggtcgcgtg gtctgggcac tttttgaggg
    10321 actaacgacc tatgatccaa aaacgctagc ccctcaaccg ggcgtcgcgg agagttggac
    10381 gatttcgcag gacaagagga cttatacgtt tcacctccgc aaagaggcgc gctggtctga
    10441 tggaaagcct ctcacggccg aggactttgt ctggtctttt cgccgactgc tccatcccga
    10501 aacggcctcc gagtacgcct atgaattgtg gcatgttgtt aacgcggaaa agtacaccac
    10561 aggccgcgtc accccgggcg atcctgtgga gatcgaactt ctggaaaaac ctcccggcgc
    10621 tccgccgttc gcctcaggga ttttgttgcg gggacggttg gtcggcattg aaccggcaaa
    10681 ttctgccctg ggcggccaga caacaggtga gcccgtttat ctggtcgaaa tcgatgggcg
    10741 aatcgaacgt tttcaaaaac catctcgcga aggccttccc cctcctgaca ccgctgcccg
    10801 cccgtatcgg tggattctct atgactttga gcatgtagga attaaggcct tggatcgtca
    10861 cacccttcaa atgacgctga cgcatccggt accgtatttt ttggagatca tcggcttctt
    10921 cccgatgatg ccggtccccc ggcattgcat cgaacaatat ggttatccca attggacgaa
    10981 gccggaaaac ctcgtttgca acggtccgtt catcctcaag taccgccgaa ttcgtgatcg
    11041 aattcgcctc gtaaagaatc tccattactg ggatcgtgag aacgttcacc ttaacattgt
    11101 ggatgtccta gcagccgagt cggccattac aggcctcaat ctctacatga ccggagcggc
    11161 tgattggatt cccgtggttc ccaatgaagt ggtgagtgaa ctccttcgcc agaagcgacc
    11221 cgattttcaa ccgtcaccct atttggctgt ggaatactat gttgtcaaca cacagcgacc
    11281 gccgctaaac gacccgcgcg ttcggcgggc actggccctg gccattaaca agcgggaact
    11341 gattgaccga attttgcaaa cgggtcaaca acctgcgttg agtttcgttc cgccctcaat
    11401 cagcgattac atcgcttatc agcccccgca aatgccagat tatgatcccg agactgctcg
    11461 ccagttgctt gcggcggctg gttatccgaa cgggcgcaac tttccaaaaa tggaaatcct
    11521 ctacaatacg ctcgagtcgc atcaagcgat tgccgaactt attcaagcac aatggaagag
    11581 gacgctgggg atcgacgtcc gattgcaaaa ccaggactgg gccaggtacc tggccagccg
    11641 acgccagggg gagttttgga tcgcgcgagc aggttggatc gccgactatc tcgatcccaa
    11701 taccttcttg gagatgatga ccacagataa ccccaacaat cacggccgct ggagccagcc
    11761 tgaatatgat cgccttgtgg ccctggccca agtcgaacca gatgaaacgc ggcggtttgc
    11821 atatttcagg caggctgaag aaatccttct ccaggaaatg cccgtcctac cgctctattt
    11881 tgccgtgacg cgggcgatgg tccgccccta tgtccgcggg tattaccaca acgtgttgga
    11941 cattcatcct ttgaagggga tgagtatcga tcacgctcaa aaagcgagcc tgctaggccg
    12001 atgaggcgaa gcccattccg tggtttcatc caacgcggca attgtggatg ccttcaagga
    12061 tatgtaaagt attagcagca cgccggcgtg ggtcatggaa gttaatttat ggcttacaac
    12121 ctttctggcc ggcagacgat ccaaattggg agacccctat ggattgcacc aggttgtcat
    12181 catcggcgct ggtcccggag gtttgggagc ggcccttctg ctggccaagg ccggtttgga
    12241 tgtgacggta ctcgagcggc tcagtgtcat tggtgggcgg tgttccccga ttaccatcaa
    12301 tggttatcgt ttcgatcgtg ggccaacgtt tctccattat ccgtgtgcac tcaagagcat
    12361 ctttcgggcc gtgggaaaag aactggaaga agaggttccc atggtccggc tcgatcccat
    12421 gtaccgcctt ttcctagggg aacgaggata cctcgatgcc accgcggaca tagcgcagat
    12481 ggaagcccat atcgccaagt ttcaaccgcg ggattgcaaa ggattcgagc agttcctcaa
    12541 agacaacgac ggcaaattcc agcgggtcat tcgcacgctc cagcgcccct ttgacggttt
    12601 gagagatctt ttctcttccg agattctgaa tctcttgccg gttttgcgac cgttcacttc
    12661 cttgcagcag gacatgagaa agtttttcag cgatccggat ttacaggttg cctttacatt
    12721 tcaatccaaa tatctgggaa tggcacctga tcgctgcccc agcatgtttt cgatcctttc
    12781 ttatctcgaa tatgcgtacg gcatttttca cccgatcggt ggatgcaatg ccgttcccca
    12841 gaagcttgcg aagttatcga aagagctcgg tgcaaagttc cacctcaacc aggaggtgca
    12901 ggaaattcgc ttcgagggcc gcaggccggt ggccgtcaaa acgcaggacg cagaatttcc
    12961 gtgtgatctc ttggtgatta acgcagattt tgcccgggcc atggaacggc tcgtgcccaa
    13021 cgttctgcgg cgacgttggt ctgatgaaaa gctgcgatca aagaagtact cctgctcgac
    13081 attcatgctc tacctgggaa tcgagggcca ggtggagaac ctccttcatc acaacatcta
    13141 catccctccc cactatcgcg aataccttga tgacgtggac gtgaggcacg tcctctccga
    13201 tgcgccggca ttctacgtac aaaatgccac gttgacagat cccacgttag cgccgccggg
    13261 acatagcacc ctgtatattc tggttcctgt tacgcatcag catcccaacg tcaactggga
    13321 tcaagaacga gcacgctatc gagaggttgt gcttgataat ctgacacgaa taggcatccc
    13381 cgatctgcga tctcgcatcc gtgctgagtt ggtctggaca ccggcagatt gggaggcgtg
    13441 gcaaatctac cgtggtgcca cattcaatct cgcgcattca cttgatcaaa tgctcatctt
    13501 ccgcccgcat aatcgatttg aggaactgga gagaacctat cttgtcggag gcggaaccca
    13561 ccccggcagc ggtttgccca ccattttcga gtcggcg
//
LOCUS       DAOSUT010000113        13521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2726_length_13521_cov_10.046265, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000113 DAOSUT010000000
VERSION     DAOSUT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2726_length_13521_cov_10.046265"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1079..2071
                     /locus_tag="PLD05_10830"
     CDS             1079..2071
                     /locus_tag="PLD05_10830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP77978.1"
                     /translation="MHHRIFPALKRTVSEIGIGTWQLGGTEWGDVPEDEALETLAAAA
                     DAGVTFIDTADIYGLGRSESLIGRFLKGRPDKDRFVVATKLGRHPEPGWPANFTREVV
                     RKHTEDSLRRLGVDVIDLTQTHSLPLEVMRDTGIFDFLRELRDEGKIRAFGASVESVE
                     EGLFCLGIEGMASLQIIFNIFRQKPIDALFDQAHQKGVALIIRLPLASGLLAGKFTRN
                     TTFPPNDHRAFNRDGQKFNVGETFAGLPFEKGLELVEQIRPWVPSGMTMAQMALRWCL
                     DFEAVTTVIPGAKRPDQARANAAASDLPMLPPILHNRLGRLYESQVAMHIRGKY"
     gene            2315..5467
                     /locus_tag="PLD05_10835"
     CDS             2315..5467
                     /locus_tag="PLD05_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP77979.1"
                     /translation="MKRCERMRPIILVIAICVVMSFCAGANLLAEEVPQATVRYNAAA
                     RLHNLQSWDLAATEWQGFIEGFPNDPRVGRAYHYLGVCLYNQQQYEKAAEAFRQSLTK
                     ELESDLRELSLLYRGIALFDAAQNGKAELYDAARSACQEYLKTYPRGKHAGEAAYYAA
                     ECLYFQDRKEQAAQAYRSFLQQFRDHPRTPEVLLALGVCEEELGNRQAALKDYQTFLQ
                     QYAQHPRATEVQYRLGEVLYALGNFAEAELAFRKAGADSRFADADLAVIRTGDTLVQL
                     KRFEEAAQQYAGVEKRFPQSSRLAQAILAAGRCYYLAGNYDSAIPLLVRASQSGEVQA
                     EAFHWLARCYLKKKQPEQVLATLREEIIGNAPAPWGAHLRLDLADALYEIQERRAEAI
                     QGYARAAELARQSKDHDLAGDALYAAAHTALTLGRLAEARQYAADFQSQYSNHALAAD
                     VLQILAECDLAEGKHDAAYQTYEKLLQGFRTHPQANLWRLRQIMALQLSGKHADVIKR
                     VEAILPQLQDKADQGTAYALLGASQLELGRLKEAQESLLLALSRDPQGSQADKSLLLL
                     ASAYFRQGRPQDARTTIEKLIKDFPRSPVLDRAYYRLGEYCFALGDWAAAEAAYRQVI
                     ERFSQSPLLPAATHELACTLIKANRFAEAEKILLDALERFPNDAITPRSRYVLGVLYS
                     QTNQPARAEEMIRKALEGGLQGTERATARYTLGLVTSAQKRYDEAASIFAQVLNDDPQ
                     YPEADKVLYQWAWALKLAGKEDEAVDLFARLAKEYASSPFRPEAEYHLAGKFYNQQDF
                     SQAAVHYHEAFQKAENPELKEKAVHRLGWCYFKMGQYDKAKQTFAYQREAFPQGSLFP
                     EAVFMEAESLFQLGKYGEALATYERLKPLDTETFEVLRLLHAGQAASRLNQWEKAFSF
                     FNQCLTKFPSVPEISQVEFEIGYIYYQQNDFKQAVEHFQRVVQKATDETAARAQFMIG
                     ESEFQQKEYDNAVKSFFRVAYGFNSPTWQAAALYEAARCFEMLKKPEQAAQIYQELIE
                     KFPQSDRIADAKAKLQMLRQ"
     gene            5551..>6006
                     /locus_tag="PLD05_10840"
     CDS             5551..>6006
                     /locus_tag="PLD05_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77980.1"
                     /translation="MSRSRLIRSWIFQQRCPLVAIGTMLFVGCAVALSPPMVCAQPPG
                     GPPRPADGAPGTSEPVNPSGNQQPDWTEADQKAAEALAAPEVPEESAPQSPAQQQVPQ
                     INVLDLAFRGGVLMIPITLMSVLTVIFGLERALGLRRRKIVPNQLIRGLG"
     assembly_gap    6007..6106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6107..6400
                     /locus_tag="PLD05_10845"
     CDS             <6107..6400
                     /locus_tag="PLD05_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP77981.1"
                     /translation="DRAEMLAQGIYTALVTTFAGLCVAIPASVLAHYFEGRIQKLLRE
                     LNESLLGLLPQLESFEGRLRIDADQLSLSDAPPKRDVPQPPPPPAPTRLGAVP"
     gene            6397..6852
                     /locus_tag="PLD05_10850"
     CDS             6397..6852
                     /locus_tag="PLD05_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP77982.1"
                     /translation="MSVLIRNKSRALESLTMTPLIDVVFNLLIFFLIASKFAEEERQL
                     PVKLPNASEAQPLVSKPRELFINIDQNGRFYVGGKTLTLAQLEKMLLEAWSANPSRTT
                     VIIRADERCRLQPVVAAINACKKANISDYRLATRKTGPPAEAASSPPSP"
     gene            6921..10292
                     /locus_tag="PLD05_10855"
     CDS             6921..10292
                     /locus_tag="PLD05_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77983.1"
                     /translation="MARPGETPRRASRSVVRKIVNEHLGDFDEQVWPVNLAILVFAGF
                     VTGIVLATTENFFDPRLLHNGFFRLGVIAVLVIGLLFSTARLKAPTARRIQFCVVLSL
                     FLHMILAVFLREQYLKLVAFLREKAAGSTTVEEVVTIPDYVESVFAEPGALSEYEKPA
                     TTAMPTERESMTEITPQAPPVPSVSEIAEESRPENVLPEEVTPLAAERQSLQTPERLI
                     EQLQSHNRQIDREATPLREEAPLQRPVELSPSKPRDMVPQDQITRQDVLPESVILRQS
                     PSASEVVLPAAPPRATTERNVENRATPPGPRQAISSQTLVSSQPEMSTPSLGGKPTSR
                     QLNPGDPALNPQATPTPLVAPVSDVPQESAWTPPAVVRSPLPTENTAQVGALAPSNPP
                     RRNPANQRDLLTSTANLDPSLAQLPAGGAASGPSSLEARESALAPTAPLANLDAAALR
                     LGTPAADDLSAATAAMIRDSGVSRLGQAQGASTGGGEGETSRAGLDTLATQPRSLRNT
                     GKTSGLPSLVSPEVGNVGVGGPVAGTGESGAPSSSGVGDKLAFRPGQLGAGQVPLLEV
                     HSDADGGPSGLLSGQGVGGVPGPTVPAMVRGSADEALGTGAGALAATGLRPGRGRASG
                     GVGLPGAGLAELAGGPDGLTGQGGGSSHGVKAGGETGSVQGGVSVADQGGFEIAAGPV
                     GETPRLLGSTGGGDETGSVTSSLLAGMPSGMPGRPERLVVSPIGRRGLIGREGLQRQL
                     GGTAELDPSEAFRQRDPAQRGSTAAQYGGTPESEAAVEKGLAFLAKIQFADGHWSLNR
                     LPRTLAGDDKTFGLGQMHGDTAATAMALLAFLGAGYTHQEPKYKDTVDRGLRWLIRNQ
                     LNNGQLFRSETDSTQYTQIYGHGLGTIALCEAYGMTRDPALREPAMKAIDYIVRSQSP
                     QFGGWRYVPRKETDTSVTGWQLMALKSAQMAGLIVPSDAFQGVARWLDQAQVDDGARY
                     VYNPYAGQTASQSHLRLPNWAMTAEGLLMRIYLGWQRDTPALRRGVEYLKQNMPSYTS
                     GSAVVRDCYYWYYATQVMFQMQGETWQLWNQRMQETLLKEQVQTGPWAGSWDPIQPVA
                     DRWGAEAGRLYVTAFHLLILEVYYRHLPLFKSLAP"
     gene            10320..11756
                     /locus_tag="PLD05_10860"
     CDS             10320..11756
                     /locus_tag="PLD05_10860"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOP77984.1"
                     /translation="MTINLPVRLLPVTEYWDCHGCGICCRHVIIPLSEEEYERIRGQG
                     WDKHPELAGHRLFVRTRFGTSKYRLAHRPDGFCVFLSPQGRCRIHEKFGADAKPLVCR
                     MFPYQLVPLERFAYLTLRHNCPSVISQQGRSLAEQEEDWRPLVENQRFQMTLTPPPPL
                     TVTYRGNWNQFLYVANAVERFLTDTRFPMVRRLAHAVIFAQLLDRCRLARLNAQQFVD
                     LVGLLERGITKEVAPLFNQRVAPNRTAQLVFRRCLIEYYRLHPGSRLEAGWRGRFAWI
                     LTSWSMATGRGRIPSLLGPERCLRVAATIPLAGEVSPWADGGQTGGNDQLPVGSEMTD
                     LAASGVENDGESAETHDGKKAFADWIDLAVLDNNLGAIHRDVLEPLDDYFEKMAISKR
                     YAVCGRKGWSLTDRVRALATSFSVGLALLRLACPDGTPTRDDVAAVVIALDRGETYRT
                     LASSIFRRRLRILDRMDQLVRLLLWYAR"
     gene            complement(11807..12958)
                     /locus_tag="PLD05_10865"
     CDS             complement(11807..12958)
                     /locus_tag="PLD05_10865"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOP77985.1"
                     /translation="MLWRYLMSCACGVVVFLAMDGQKLMGEEATFWVYYGTYTRDTSR
                     GIYVGKFDANTGRLTEPELAAETRNPSFLAVHPTRPLLYAVGELAEFEGQRTGAVLAY
                     QIDSQSGKLVAINRQPSGGTGPCHLVVDPSGRFVLVANYGGGSVASLPIRPDGGLEPP
                     ACVLRQEGRSVHPTRQTAPHAHQINFSPKGDLVLVPDLGLDKVLLFRWSPADGQLTEY
                     DPPFVTVPAGSGPRHFAYHPTGRALYVLNELTATVCVFECASGVPTRLVETVSSLPQD
                     FSGSNTAAEIVVHPTGRFLFTSNRGHDSVAVFRIADDALKISLMENVSVQGKTPRFIG
                     IDPTATFLFAANQDSHNVVVFRIDVESGHLTPTGQVINLGSPVCMIFRK"
     gene            complement(13109..13342)
                     /locus_tag="PLD05_10870"
     CDS             complement(13109..13342)
                     /locus_tag="PLD05_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77986.1"
                     /translation="MRHTKLRINPTIWALSAIGLLGLFSFAGCQSRPPLEELGELEFA
                     VPDIPGIDDPYPFPDVAGSAEKPADSEQSDDEP"
BASE COUNT         2862 a         3735 c         3936 g         2888 t
ORIGIN      
        1 aaggcgtgga agaacatctg ccgcggtaac aagaactacg acatctacgt tggaaagagt
       61 gcgtcgccat cgttgcagga aaatagcttg gagaaacgaa gtgacaaaaa cacggcgaaa
      121 caagagagtg gctaaatagt gttctgccgg aagcgtagtc aatcgtcggg gcaaccccct
      181 gtggttgccc catttgtccg cagcgccggt ggtttgcgtt tggccgggcc cggattgttt
      241 ggatttcaca cgcacccggg cgttattaac gctaatcgca ttttggggca ggcacggggg
      301 cctgccccta cggttgttgg ttttctattc gcgaagccgt aggggcaacc ccctgtggtt
      361 gccctgtgtg tcgacagcgc cagccggaat gatcgccgtc ttgggcgaca caatttggcg
      421 gtttaggaac gtagggtcat cgtccacaat gccccaaaca tcggccaaca ccgacgccga
      481 ttcccggcgc cctggggcag gcacgggggc ctgcccctac ggttgttggt tttctattcg
      541 cgaagccgta ggggcaaccc cctgtggttg ccctcgtcgt ccgcatggct ggtggtttgc
      601 gtttggccag gtgcccaggt tgtttggatt tcgtactgac ccgcggggta tttgtgctgc
      661 ggggcaggca caggggcctg cccctacggt tctgatgggc ccctattcgc catggtcaac
      721 gggtatatcg aaccggacgg cgacgaccgc gtccctccca cgggcgcgca gaaaaacgcg
      781 gtggacgcga cggagcgagt ccctcccaaa acggcggtgg tggagggcca cgcttcgtct
      841 tgggcagctt gaccatttcc ctgtacgtgt accttgtacg tgcttgcgtt gtggcatcct
      901 ctcgggcaaa ctgagattta cgcaagatga cggtacttgg catcgatcag tggcgaagaa
      961 agtgagcccc gtcccgtgtc aactgtaagg agttctctgc cctggagtgt agtatttccg
     1021 catcaaaggt gtccgcatcg caaacgaaaa ccttgatcgt ttcccaaaag gaggtgacat
     1081 gcaccaccgc atatttccag cccttaagcg aacggtttct gagatcggaa ttggcacatg
     1141 gcaactcggt ggcaccgagt ggggtgatgt tcccgaggat gaggccctgg agacgctggc
     1201 cgcggcagcc gacgccggtg tgacgtttat cgacacggcc gacatttacg gtcttgggcg
     1261 gagcgaatct ctcattggtc gttttctgaa aggccgcccg gataaggacc gattcgttgt
     1321 cgcgacgaaa ctcggtcgtc accccgaacc gggctggccc gctaacttta cccgcgaggt
     1381 tgtccgaaag cacacggagg actctttgcg acgcctgggg gtcgatgtga tcgacttgac
     1441 gcaaacccac agtttgccct tggaagttat gcgggatacg gggattttcg atttcttgcg
     1501 cgaattgcgg gacgaaggca aaatccgcgc ctttggagcg agcgtggaaa gtgtcgaaga
     1561 aggcctgttc tgtcttggca tcgaaggaat ggcttcactt cagatcattt tcaacatctt
     1621 tcgtcagaaa ccgatcgacg cccttttcga tcaggcacat caaaaggggg ttgctctgat
     1681 tattcggctg cctttagcca gtggtttgtt ggcggggaag ttcacacgaa acaccacgtt
     1741 tccgcccaac gatcatcgtg cgttcaacag ggatggtcag aagttcaatg tgggagagac
     1801 atttgcgggg ttgccctttg aaaaagggct ggagttggtg gagcagattc ggccatgggt
     1861 cccttccggg atgacgatgg cccaaatggc cctccgctgg tgtttggatt tcgaggccgt
     1921 gacgaccgtc atccccgggg caaagcgacc cgaccaagcc cgcgccaacg ccgctgccag
     1981 tgatttgccc atgctaccac caatcttgca caaccgcctg ggacgattgt acgaaagcca
     2041 ggtggctatg catatccgcg gcaaatattg aaaaggggtt tgctttgccg acgggggttg
     2101 tggcgggcac cgccggttcc cctccaccgg cgaaaaagca ctttccgagg gacgtttgga
     2161 cgcgatatga tgacgaaagg cgttttcgtc atcccgtttg gtgttgcgct gtcgggaatt
     2221 catctttctg cccaccggcg ccgacgcgaa ttgtaagtcg cggccggaat ggattattgc
     2281 cctggcgacg tgatgcaaga ggagtgagga gggcatgaaa cgttgcgaac gtatgagacc
     2341 aatcatactc gtcatcgcca tttgtgtggt aatgagtttt tgcgccggcg cgaatctcct
     2401 ggccgaggaa gtacctcagg cgaccgtccg gtacaacgcc gcagcgcgac tacataatct
     2461 ccaatcctgg gatctggcag caacggagtg gcaaggtttc attgaggggt ttcccaacga
     2521 tcctcgggtg gggcgagcat atcactattt gggggtctgt ttgtacaatc agcagcagta
     2581 cgaaaaggcg gccgaggcct ttcgacagtc gctgaccaag gaacttgaga gcgatctgcg
     2641 cgaactttct ctgttatacc gagggattgc cctattcgat gcggctcaaa acggcaaggc
     2701 ggaattgtat gacgcggccc gttctgcctg ccaagagtat ctgaaaacct atccgagggg
     2761 aaagcatgcg ggtgaagcag cctactatgc ggccgaatgc ctctactttc aggaccgcaa
     2821 agagcaggcc gcgcaggctt accgaagttt tcttcaacag ttccgtgatc atccgcggac
     2881 gcccgaggtc ttgttagccc tgggagtgtg tgaggaagaa ttggggaatc gtcaggcggc
     2941 gctgaaggac tatcagactt ttttgcagca atacgcgcag catccgcggg ctaccgaggt
     3001 tcagtatcga ttgggagaag tcctgtatgc cttgggcaac ttcgctgagg cggaacttgc
     3061 ctttcgcaag gccggggctg attcgcgttt cgccgatgcc gacttggccg tgatccgtac
     3121 tggcgatacc ctcgtccaat tgaagaggtt tgaggaggcg gctcagcagt acgccggcgt
     3181 ggaaaagcgg ttcccgcagt cctcacgact ggctcaagcg attctggcgg cgggccgatg
     3241 ttattacctt gccggtaatt atgattcggc aattcctctc ttggtgcgcg cgtcgcaaag
     3301 cggtgaggtg caggccgagg ccttccattg gttggcccgc tgttacctca agaagaaaca
     3361 gcctgaacaa gtgctcgcca cattgcggga agagattatc ggtaacgcgc ccgcaccttg
     3421 gggagcccat ctgagattgg atctggccga tgccttatac gagattcagg aacgtcgggc
     3481 agaggccatt cagggatacg cccgggctgc ggaactggcc cgccaaagta aggaccacga
     3541 tttggcgggt gatgccctct acgcggcggc gcacacggcg ctcacgttgg gacgccttgc
     3601 ggaggcgcgc caatatgcgg cagattttca gtcgcagtac agcaatcacg cactggcggc
     3661 cgatgtcttg cagattctgg ctgagtgcga tctagccgag ggcaagcacg atgcggccta
     3721 tcagacttat gagaagcttc tccaaggttt ccgcactcat ccccaggcca acttgtggcg
     3781 gcttcgtcaa atcatggcac tccagcttag tggaaagcat gcagacgtga tcaaacgggt
     3841 cgaagccatc ctcccgcaac ttcaggataa agccgatcag ggcacggcct atgccttgtt
     3901 gggagccagt cagcttgaac taggacgatt gaaggaggcc caggaatcgc tcctcctcgc
     3961 cctcagtcgc gaccctcaag gttcacaggc agacaagagc ctcctgcttc tcgcctcggc
     4021 gtattttcga caaggacgcc cacaggatgc gcggacaacg atcgagaaac tcattaagga
     4081 tttcccacgt agcccggtac tcgatcgggc gtattaccgc cttggcgaat actgttttgc
     4141 cctgggtgat tgggcagccg ccgaagccgc ctatcggcag gtaatcgaac ggttttcgca
     4201 aagtcccctt ttgccggcgg ccacgcacga attggcttgc acgctcatca aagccaatcg
     4261 atttgctgag gctgagaaaa tcttgcttga tgctcttgaa cggtttccca acgatgccat
     4321 cacgccgcga tctcgatacg tgcttggtgt cctgtatagc cagacaaatc aacctgctcg
     4381 agcagaggag atgattcgca aggccctgga gggtggtttg caaggcacgg agcgcgccac
     4441 ggcccgctac acacttggct tggtgacaag tgcccaaaag cggtacgacg aggctgcgtc
     4501 gatcttcgcc caggtcttga atgatgatcc gcaatatcca gaggccgata aggtcctgta
     4561 ccaatgggca tgggcgctta agttggcagg aaaagaggac gaagctgtcg atttgttcgc
     4621 acggttggcg aaggagtatg cctcaagtcc gttcagacct gaggccgagt atcatctggc
     4681 tggtaagttt tacaatcagc aggatttctc acaggcagcc gtgcattatc acgaggcatt
     4741 ccaaaaggcc gagaaccctg aactcaagga aaaggctgtc catcgcctcg gttggtgcta
     4801 tttcaagatg gggcaatatg acaaggccaa acagacattc gcttatcaga gagaagcttt
     4861 tccgcagggt tcgctgttcc ccgaggccgt gttcatggag gcggaatcgc tctttcagtt
     4921 gggtaaatat ggcgaggcct tggcaactta tgagcggctg aagccactgg ataccgagac
     4981 tttcgaggtt ttgcgtctcc ttcacgcagg tcaggcggcc tcgcggttaa atcaatggga
     5041 gaaggccttc tcgttcttca accagtgcct gacgaagttt ccttctgtcc cggaaatttc
     5101 ccaggtcgag ttcgaaatcg gttacattta ctatcagcag aatgatttca aacaagccgt
     5161 ggagcatttt cagcgggtag ttcaaaaggc caccgacgaa acggcagccc gcgctcaatt
     5221 catgattgga gaaagcgagt tccaacaaaa agagtacgac aatgcggtga agagcttctt
     5281 ccgcgtggcc tacggtttta atagtcccac atggcaggca gcggcactgt acgaagccgc
     5341 acgctgcttt gagatgttaa agaagcccga acaagcggcg cagatatacc aggagttaat
     5401 cgaaaaattt ccgcagagtg atcgcatcgc cgacgctaag gctaaactcc agatgctcag
     5461 gcagtaacgg gataaccgat aaaaagaagg tatgggtaga aacccgtcgc cttctgccgt
     5521 cggttactga tacataagcg agaaccgaaa atgtcgcgtt ccagactcat cagatcttgg
     5581 atattccagc aaagatgtcc gctggtggcc atcggcacaa tgttgttcgt agggtgtgcc
     5641 gtggcgttat cgcccccgat ggtgtgtgct cagcccccgg ggggcccacc ccggccggcg
     5701 gatggtgctc caggtacaag cgaaccggtc aatccatctg gtaaccagca acctgattgg
     5761 accgaggctg atcaaaaggc tgcagaggcc ctggctgccc ccgaagtccc cgaggaatct
     5821 gccccgcaat ccccggctca gcagcaggtt ccgcagatta acgtgctaga tttggccttc
     5881 cgcggcggtg tgttgatgat tcccatcaca ctgatgtcgg ttttgaccgt catctttggt
     5941 ttggagcggg cccttgggtt gcggcggcga aagattgttc ctaatcagtt gatacgagga
     6001 ttgggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatc gggcggaaat
     6121 gctcgcccaa gggatctata cagctttggt gacaactttt gccgggctgt gcgtggccat
     6181 tcccgcttcc gttttggccc attattttga gggacgtatt caaaagcttt tgagagaact
     6241 gaacgagtcg ctccttgggc tcctgcccca gttggagtcg tttgagggtc ggcttcgcat
     6301 tgatgccgat cagttgtcgt tgagcgatgc cccacccaag cgggatgtgc ctcaaccgcc
     6361 gccaccgcct gcaccaacgc gtttaggagc cgttccatga gcgtcctcat ccgcaataaa
     6421 agccgtgccc tcgaatccct cacgatgaca ccactcatcg acgtggtgtt taacctgctc
     6481 atcttctttc tcatcgcaag caagtttgcc gaggaagaac ggcaactgcc tgtgaaactg
     6541 cccaatgcca gcgaggctca gccgttagtc agtaagccga gagagctctt catcaacatt
     6601 gaccaaaacg gcagatttta tgtgggggga aaaacgctca cgcttgccca actggagaaa
     6661 atgctcttgg aagcgtggtc ggctaacccg agtcgaacaa ccgtcattat tcgggccgac
     6721 gaacgctgtc gattacaacc ggtggttgcc gcgatcaacg catgcaaaaa ggccaatata
     6781 tcggattatc gtttggcgac acgtaagacg ggcccgcccg cggaagcggc gtcaagccct
     6841 ccgtcgccgt aacgttaaga tgtgatggct gggggatttg gagtggttcg ctcagttttg
     6901 tagctcgatt gtgtgcccca atggcacgtc caggtgaaac accaagacga gcgtcgaggt
     6961 cagttgtccg taagatcgtc aacgaacacc tcggcgattt tgatgaacag gtttggccgg
     7021 tcaacctggc catcctggtt ttcgcgggct tcgtgacagg gattgtccta gccaccacag
     7081 agaatttctt cgatccacgg ctactccaca acggattttt ccgccttggg gtgattgccg
     7141 ttctcgtgat tggtcttctt ttcagtacgg ccaggctcaa ggcaccgacg gcacgacgca
     7201 tccagttttg cgtggttctc agcctgtttc tccatatgat cctggccgtg ttcctccgcg
     7261 agcaatattt aaaactggtg gcgtttctgc gtgaaaaggc agccggaagt acgacggtgg
     7321 aggaggtggt caccatcccc gattacgtcg aaagtgtgtt cgccgagccg ggggccttgt
     7381 cggaatatga aaaacccgcg actacggcca tgcccacaga acgcgagtca atgacggaga
     7441 tcactcctca ggccccccct gtcccctctg taagtgagat tgcggaggaa tcccgtcctg
     7501 agaacgtgct tcctgaagag gtcacaccct tggcggctga acgccagtcg cttcaaaccc
     7561 cggaacgact catcgaacaa ttgcagtccc acaatcgtca gatcgatcga gaggcgacac
     7621 cgcttcgtga ggaggccccg ctccaacggc ccgtagagtt gagtccctcc aagccgcgtg
     7681 atatggtacc ccaagaccag atcactcgcc aggacgttct tccggaatcc gtgatcttgc
     7741 gacaatctcc gtccgcttcg gaggttgtct taccggccgc tccgccacgg gcaaccaccg
     7801 aaagaaacgt cgaaaatcgt gcgaccccgc ctggacctcg tcaggccatc tcttcacaga
     7861 cacttgtgag tagccaaccg gagatgagta cgccttcact gggtggcaaa ccgacgtcac
     7921 gccaattgaa tccaggtgat cctgccctca atccgcaggc aacgccgacc ccgctggttg
     7981 cgccggtttc cgacgtaccc caggaatcgg cctggacgcc acccgccgtg gtgcgatcac
     8041 ccctacctac cgaaaacacc gcgcaggtgg gagccttagc cccgtccaac cccccgcgac
     8101 gcaatccggc gaaccaacgg gatcttttga cctcgacggc caacttggat ccttctttag
     8161 cccagttgcc ggcaggcggc gcggcgtccg ggccgtcatc gcttgaagca cgtgagtcag
     8221 ccctcgcccc caccgctccg ctcgccaatt tggacgccgc cgccctgcga ttgggaacac
     8281 cggcggccga cgatctgtct gcggccacgg ccgccatgat ccgcgattcc ggcgtttctc
     8341 gattgggcca ggcgcaaggt gcgagcacgg gaggcgggga gggggagacc tcccgggccg
     8401 gcctcgatac gctggcaact cagccgcggt cgctgcgaaa tacgggcaag acgtcgggac
     8461 ttccctccct cgtttcgccc gaagtgggta acgtcggggt cggtggaccg gtggccggca
     8521 ctggagagag cggggcacca agctcaagcg gagtgggcga caaattggcc tttcgaccgg
     8581 gtcaactggg agcagggcag gtaccgcttc tggaagttca ttcagacgcg gacggcggac
     8641 ccagtggcct tctaagcggt caaggcgtgg gcggcgtacc cggaccgacg gtcccggcaa
     8701 tggtcagagg ctcggctgac gaggccctcg gcacgggggc gggcgcgctc gcagcaactg
     8761 gcctgcgtcc tgggcgaggc cgcgcctcgg gaggggtagg ccttcctggg gcagggttgg
     8821 cggagctagc gggaggaccc gatggtctga ccggtcaggg aggcggctcg tcgcacgggg
     8881 taaaagcggg cggcgagacg ggttcggttc agggaggagt gtccgtggct gatcaagggg
     8941 gatttgaaat cgcagcgggc cccgttggtg aaacaccccg gttattggga tcaacgggtg
     9001 ggggagatga aacgggcagt gtaacttcat cgttgctggc cgggatgccc tcgggtatgc
     9061 cgggacggcc ggagcgactg gtagtgagtc ccatcggccg acgtgggctc atcgggcgag
     9121 agggacttca gcggcaactg ggcggaacgg ccgaacttga tccctcggag gcgttccgtc
     9181 aacgtgaccc ggcacagcga gggagcaccg ccgcccagta cggaggtacg ccggaaagcg
     9241 aagccgccgt ggaaaagggc ctggccttcc tcgcaaagat ccagttcgcc gacggacatt
     9301 ggagtttaaa ccgcttgcca cgaacgctgg cgggcgacga taagaccttt ggcctcggtc
     9361 aaatgcacgg cgatacggca gccaccgcca tggcactgct cgcctttctt ggggcgggtt
     9421 acacccatca ggaacccaaa tacaaggata cagttgatcg cggcttacgc tggttgatca
     9481 ggaaccaatt gaacaacgga caattatttc gctctgaaac ggattcaacg caatataccc
     9541 agatctatgg ccatgggctg gggacaatcg ccctttgtga ggcctatggc atgacgcgcg
     9601 atccggcatt gcgtgaaccc gccatgaagg cgattgacta tattgtgaga tcgcaaagcc
     9661 cgcaattcgg tggctggcgc tatgttccgc gcaaggagac ggatacctct gtgaccggtt
     9721 ggcaattgat ggcgctgaag agcgctcaaa tggcaggtct gattgttccg agcgacgcat
     9781 ttcaaggcgt tgcccgctgg cttgatcaag cacaggtcga tgatggggca cgctatgttt
     9841 acaatccgta cgccggacag acggcgtcgc agagccattt gcggttgccc aattgggcca
     9901 tgacggccga gggacttctt atgcgaattt atctcggttg gcaacgggat acaccggctt
     9961 tgcgtcgcgg cgttgagtac cttaagcaga acatgcccag ttacacaagc ggctcggccg
    10021 tggtgcggga ttgttactac tggtattacg ctacccaggt catgttccaa atgcagggcg
    10081 agacctggca actgtggaac cagcggatgc aggaaacatt gcttaaggag caggtccaaa
    10141 cggggccgtg ggccggtagt tgggacccga tccaaccggt ggccgaccgc tggggagcag
    10201 aggccggtcg cctctacgtg acggcattcc atctcctcat cctcgaggtt tattatcgac
    10261 atttgccctt gttcaaatcg ctcgcgcctt aaagcgactg gaagaacata tcttaaccca
    10321 tgaccatcaa ccttcccgtc cggctattac ccgttacgga atattgggac tgccacggct
    10381 gcggtatttg ctgtcgccac gtcatcatcc ctttgtcgga ggaggaatac gaaagaatcc
    10441 gtggccaagg ttgggacaag catcccgaac tggctgggca tcgtctgttt gtgagaaccc
    10501 gttttggaac atccaagtat cgactggctc atcgtcccga cgggttttgc gtctttctaa
    10561 gccctcaggg gcggtgtcgt attcacgaga aatttggtgc cgacgccaag cccttggttt
    10621 gccgaatgtt tccctatcag cttgtcccac tcgagcgatt tgcctaccta acgctgcgtc
    10681 acaattgtcc cagtgtcatt tcgcaacagg gccgatcgct tgccgaacaa gaggaggatt
    10741 ggcggccact ggtagaaaac cagcgttttc aaatgacctt aactccaccg ccgccactca
    10801 ccgtgactta tcggggaaat tggaatcaat tcttgtatgt tgcaaatgcc gtcgaacggt
    10861 ttctgacgga tacccgtttt ccgatggtac gacgtctggc tcacgccgtg atattcgcgc
    10921 agcttctcga tcgttgccgg ttggctcgct taaatgccca acagtttgtt gaccttgttg
    10981 gcctgctgga acgcgggatc acgaaagagg ttgctccgtt atttaaccaa cgcgtagccc
    11041 ccaatcggac ggcgcagctc gtgttccgca ggtgtttgat tgagtactat cgcctccacc
    11101 ctggttctcg cctcgaagcc ggctggcgag gacgcttcgc ttggatccta acgtcatgga
    11161 gcatggcgac tggccgcggg cggattcctt cccttcttgg acctgaacga tgcctcaggg
    11221 ttgccgccac gataccactt gcaggtgaag tatctccgtg ggctgacggt ggacaaacgg
    11281 gaggaaatga tcagctccct gtgggttcag aaatgacaga tctggctgca agcggtgttg
    11341 aaaacgatgg ggaatctgcc gaaacccatg acggcaagaa ggccttcgcg gactggattg
    11401 atctggcggt gttggacaac aatttagggg ccatccaccg agatgtcttg gagcccctcg
    11461 atgattattt cgaaaagatg gccatttcaa aacgatatgc cgtttgcggt cgaaagggct
    11521 ggtcgctaac cgaccgggtg cgagccttgg ccacatcctt ttcggttggt ttggcccttt
    11581 taaggctggc ttgtcccgac gggactccca cccgcgatga cgtggccgcc gtggtcatcg
    11641 ccctcgatcg tggcgagacc taccgcacac tggccagttc gatctttcgc cgccgactcc
    11701 gcatcctcga taggatggat cagcttgtca gacttctctt gtggtacgcg cgttaaattg
    11761 atctcaggtg aagcctcttt ggccaattgg cgcgacaaaa gggctgtcac ttccggaaga
    11821 tcatacacac cggtgagcca agattgatta cctgccccgt cggtgtcaga tgtccggatt
    11881 ccacatcaat acggaagacc acaacgttgt gggaatcctg gtttgctgcg aaaagaaacg
    11941 ttgccgtagg atcaatgcca atgaagcgtg gggttttccc ctgcacgctg acattttcca
    12001 ttaggctgat tttcaaggca tcgtcggcga ttcggaacac cgcgacggag tcgtgccctc
    12061 ggttcgaggt aaagagaaag cgacccgtcg ggtgtaccac gatctctgcg gcagtgttcg
    12121 agcctgagaa atcctgcggc aacgacgaca ctgtctcaac caatcgggtt ggcacaccgg
    12181 atgcacattc aaacacgcag accgtggccg tcagttcgtt aaggacatac agggcccgcc
    12241 ctgtgggatg ataggcgaaa tgccgcggac cggagcctgc cgggacggtc acgaagggtg
    12301 gatcatattc cgtgagttgc ccatcggccg gcgaccatcg aaacagaagt actttgtcca
    12361 gtccaagatc gggcacgagg acaagatccc ctttggggct aaagttgatc tggtgggcat
    12421 ggggggccgt ctgcctggtc gggtgaacgc tccgcccttc ctgacgtaga acacaagcgg
    12481 gcggttcgag tcccccgtct ggacggatcg gtaggctggc aacactacct cccccgtaat
    12541 tggcgaccaa cacgaatcgt cccgaaggat ccacaacgag atggcatggc cccgttccgc
    12601 cagaaggttg ccggttaatg gcgacgagtt tcccggattg actatcaatt tgatatgcca
    12661 aaaccgcccc ggttcgctga ccctcaaact ccgccaactc acccacggca taaagcaggg
    12721 gccgcgtggg gtgaaccgcc aagaaagaag gattccgcgt ttccgcggct agttccggtt
    12781 ccgtcagtcg ccccgtgttg gcatcgaatt ttccaacata gataccgcga cttgtgtctc
    12841 gcgtgtatgt cccataatag acccaaaagg tggcctcttc gcccattagc ttttgcccat
    12901 ccattgctag aaaaacaacc acgccacatg cacaggacat aaggtatcgc cacaacattt
    12961 gatggcctcc tattggctac tcttgggtcc cacgttgcgt ttcatccgca gagatagatt
    13021 accgaagtat ttcgcgactc acccacggcc aacgcgcttt acttgtatag accactcgaa
    13081 caactgcgaa cgcttgttgg ccggttgatc acggttcatc gtccgactgt tccgaatctg
    13141 ccggtttctc ggcactgcct gcgacatccg ggaacgggta cgggtcatcg attcccggga
    13201 tatccggcac ggcaaattcg agttcgccca actcttctag gggaggccgt gactggcacc
    13261 ctgcgaaaga gaataggccg agcaaaccga tcgcactcag tgcccaaatt gtcgggttaa
    13321 tccgcagctt tgtgtgtctc atgatcatcg ttcttgtata atccctatgg acttaaatga
    13381 tcccaaagta ttcccaagta tagctatagc gcgtcgcagt gcatccgatg accacaccgt
    13441 tttgacggcg gaaacgaaac cgcgacagca acgatcgatt gggtcatcgc gaattaagag
    13501 cctatctcgt aatctcccct c
//
LOCUS       DAOSUT010000114        13415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2754_length_13415_cov_9.933383, whole genome shotgun sequence.
ACCESSION   DAOSUT010000114 DAOSUT010000000
VERSION     DAOSUT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2754_length_13415_cov_9.933383"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..706)
                     /locus_tag="PLD05_10875"
     CDS             complement(68..706)
                     /locus_tag="PLD05_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77987.1"
                     /translation="MGNSVSRWDKSAFGLMDPAAPDSGGRLTFTLLYEVGIEGNPHRW
                     RFILRSPDGHDELIAEDVEPGVQGERLELLTVLRALESLNQSSRIIIWTRSPALKEGL
                     LYGLRDWPLHDWCWERFGERVPVRNRDLWQRLERVIQFHEIECRAWRFDPPHVHEVSP
                     PNSRGFGQRAFAPGKPKSGCQNGQGDSPGGSSWKWLFGAFLPRRSTVSNLPK"
     gene            complement(944..1936)
                     /locus_tag="PLD05_10880"
     CDS             complement(944..1936)
                     /locus_tag="PLD05_10880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP77988.1"
                     /translation="MIASSRREFLQTVLAGLALGRMAFASQETSAGGLPTRPLGNTGE
                     RVTIIGLGGWDVGAVREKKEALAIMHEAVDNGLTFFDNCWEYHQGRSEEWMGEAMAEG
                     KYRDKVFLMTKVCGRDAKTAQQNLEDSLRRLRTDHIDLWQFHGIQWDDDPDLIFGENG
                     ALAYALKAQKEGKVRYIGFTGHKHPRFHLAMLQKPYEWASVQMPINVLDAHYESFQKQ
                     VLPECVKRNIGVIGMKALGSQNGRIPRELGIPAPLLRRFALSLPITSLICGIQSRENL
                     RQDLAMARDFQPLSEEEVKELLAKAAGPAASGQIEQYKVGNYGCDWWHNLQKQG"
     gene            complement(2162..3037)
                     /locus_tag="PLD05_10885"
     CDS             complement(2162..3037)
                     /locus_tag="PLD05_10885"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOP77989.1"
                     /translation="MEKRLTRRVALSSLGLISGGILASGCTKGLVAPVSAEPVTPAAN
                     GNGLGASSEADHGWKYAPLNAEEIADRAYAMYPDGGCMYSMFGSVISALGERFGEPFS
                     QFPYSMMRYGEGGVGKWGSLCGVVNGAAALIGLFYPEDQNTREKLIEAFAYWYESSEL
                     PVYVPKDAPQSMEIPKSVAGSILCHFSVGAWCKVSGYPAFCKEKTERCRRLTADGVRF
                     LVTILNESHMQTNRELPTSPTAKACNDCHGKAQLADAMVKMNCQSCHDLDPSHSVQPV
                     TNGTEFNPAQIRSGR"
     gene            3676..4215
                     /locus_tag="PLD05_10890"
     CDS             3676..4215
                     /locus_tag="PLD05_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77990.1"
                     /translation="MLGFVHPIIALEQSKFQGGLLVLNQSGDPVEFRCTVPVRTTRLQ
                     QILWGQRLVAHVLGQLILRPLIQSAKTQIAVVLVEREEFLPVRGDLSVPLLLIRRQDH
                     PSDRIQSPNTVTIAHVSPGKEALLLQSAWGFEEDLEVGCRLVSEFLKTCHPLEAFQRI
                     EQALHILKPKLEEQVRVTG"
     assembly_gap    4763..4862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4863..5085
                     /locus_tag="PLD05_10895"
     CDS             <4863..5085
                     /locus_tag="PLD05_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77991.1"
                     /translation="CGQPPVVAFFFLRNLNDFADYSKGAETAARHDLFGWQWGPGSSW
                     RPCRCRGITVVRDRDSTTGQLRLTEDRFR"
     gene            5187..6506
                     /locus_tag="PLD05_10900"
     CDS             5187..6506
                     /locus_tag="PLD05_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP77992.1"
                     /translation="MWKAVTTTLNRALTSPLLWGSAAFVAFQFFLKADLPGGDFLRRY
                     TAGHPIEYIETFLFFLALAALIHKGLTILYEWSTLDQPWLSGPLNAPADRLLGDLDAL
                     PEVLHTSPVFRQLRAGLSYLCRGGTAQELDQHLAFAAEQEADERRHHYAFVRLVIWAI
                     PILGFLGTVVGITLSLGNLAPQQLEETLPAVMAGLMVAFDTTALALALSMILMLVQYV
                     VERSEQRLLAAAGEKVENLLLSRFPAQSVPGRELTAVRVMLEEVLTTCRHLVETQVTL
                     WQQSLEQAQKQWQGTWSNWQEQLGNRLATALENALQRHADRLHEAEAQLFERLQQREL
                     EAAQDLTARWKSLLTEASRQQEEWASLQKILQGHVTAWQTLASETQHLARLEELLQQN
                     LTAIGSAKHFEEALASLAAAANLLSAHLHRIPLEPPRAERPRRAEAA"
     gene            6503..8188
                     /locus_tag="PLD05_10905"
     CDS             6503..8188
                     /locus_tag="PLD05_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77993.1"
                     /translation="MNWRRRKHSKSGENPISLFPFLAVLICTMGVLILLLVILVRQAR
                     LKALAQAQASSNPASQKEVSSPIPTEDELKRWEEDLAWQEEQLRTAREKTQAQLTEAR
                     LILGHIEDSIRTFRQELQSLQERLQRLNEVTNESGSVSIAELENHLHELHEAAQSLKA
                     QVEAKEHQRRRKSYAIIPYQGKFGTARRPIYLECRGDGILLQPEGIRFTVQDFLGPLG
                     PGNPLDAALRAIREELLARKEFDPQADGEPYPLLIVRPDGIEAYYAARAAMSSWGTEF
                     GYELVEKDWELVYPQPDPRLAEVVQQAVDLARKRQEFLVKAAPGKYGRTPRQYRAAPY
                     VGGAVPTEAPATPPEDPMLAQWLARQQSGNTAVRPESAPGGAAATSRTGNSTSEQAST
                     QSPVERQVEDHVTISDLRVMSPHRPVGDQPQVGGAPSPKESTAVEITTGVTLRPLAEQ
                     RGRNWGLPPHAASAIPVTRPIRIDCFPDRVVLIPEQGLDHIRVFYLTPSPAKAVDELV
                     KAIREYVETWGIAGNNMYWRPVLSVRVAPGAEAQFQILQILLKDSGLTVEQTR"
     gene            8255..9289
                     /locus_tag="PLD05_10910"
     CDS             8255..9289
                     /locus_tag="PLD05_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP77994.1"
                     /translation="MKNTERDGQINAGYDSFLDTVTNIVGILIILVMVVGVRAQHLPF
                     TLKPDTSHAESAEEQARRARAEELARRSTALLSLRRDILELSATVEMVEAERLARERE
                     RQRLALAAALLKKELDEKTVALSQEERRREQLAEAIAACERDIAASQQRLAELSSTTE
                     QVQIVNHPTPLSVPVDFEEIHFVIRQGKIAYIPLSSLLDELMSDARRKIPRLFEVPEI
                     TGTVGPVGGFKLEYSIVRRTIGEAEIAGVPGAGAYAQLRRWILIPEGNVGEPVAQALE
                     ESSEFRRRIAGYRPDRTTVTIWCYPDGFADFRRLKDFLHERGYSVAGRPLPDGFPITG
                     SPGGTKSSAQ"
     gene            9481..10518
                     /locus_tag="PLD05_10915"
     CDS             9481..10518
                     /locus_tag="PLD05_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOP77995.1"
                     /translation="MAPAVKPIPLNRAIAPFFVGVDLGGTSTKIGLVDDLGRPVCQPQ
                     EFPTAVEDGPEQLVARVAEVINQMIQTSGLESTEIARVGLGCPGILDLHTGVMVNPTN
                     FPGWGGFPIREALADRSGMPVVMANDASAAAYGELWIGSGRGFRSLVLLTLGTGVGCG
                     AIIGDVIIEGEHGHGTECGHILIDPSDTAPVCSCGQRGHLEAFASATAVVRRAREKIA
                     AGEAPLLAEKVSNGEKLSPLLLAKLAEAGDQPSLDLILETARYLGLGIVTLLHTFDPS
                     GLLLGGAMTFGGDKSPVGRRFLERIREEVRRRAFAALANKVAIRFAALGGDAGFIGAA
                     GIARLHHRRYR"
     gene            complement(10533..11453)
                     /locus_tag="PLD05_10920"
     CDS             complement(10533..11453)
                     /locus_tag="PLD05_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP77996.1"
                     /translation="MLLRFLIDRLIWLAITLWVVFTVSFFLMRAVPGGPLDRERRLDP
                     QIEKNLAARYHLDEPVWQQYLRELGHVVQFDLGYSYRMADFTVNEVIAQGLPISAALG
                     LAAMGLALTLGFAAGVASAAFRNSVLDRGLMVLATVGLAVPNFILAGVLIMLLVFVLP
                     CFPAAGWGRPSHLVLPAVCLGLPYAASIARLTRTGLLEVLTQDYIRTARAKGLNQVTI
                     IFKHALPGALAPVVSFLGPAVAGIITGSPVVEKIFAIPGLGMHFVEAALQRDYTLSMG
                     LVLLFTLLLYGANVLVDLAYTILDPRVELQ"
     gene            complement(11544..12389)
                     /locus_tag="PLD05_10925"
     CDS             complement(11544..12389)
                     /locus_tag="PLD05_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HOP77997.1"
                     /translation="MASDNDTSPRPEVSLQPEEGWHCQHWFYRFDRMRLRTLAPGEYT
                     DGCKDFCETLDPRSPEAPMRLQTFVIMGHKADFGILMLDPDPLKLDRVHQRLLAGLLG
                     PTIEPMWSFVSMTEVSEYVPTVEEYAARLQQEGLPPDSPEHQERTRQYARRYEIMRRQ
                     RLQPDLPNWPAFCFYPMNKRRLPEANWFLLDFEQRDRLMREHGETGLKFAGRVTQLVT
                     VGIGLDDWEWGVTLWARNPQFLKDIVYRMRFDEASARYAEFGPFYSGYQATPQQILSQ
                     CRVCR"
     gene            12966..>13415
                     /locus_tag="PLD05_10930"
     CDS             12966..>13415
                     /locus_tag="PLD05_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOP77998.1"
                     /translation="MLNRHLADKILRLLTEERLSQRQVARLTGVSRATIAAIAKGEWH
                     ARFRERPKDESDQPPAPSRCPECGALVTPPCLACRIRAMRIRGEILPLPGEGPGPLKL
                     ELRGPHYARYLEVRAAAMARGEGEPLEDPTEDAADDDAWSDEAFWADE"
BASE COUNT         2864 a         4218 c         3615 g         2618 t
ORIGIN      
        1 cctctcccgg ggggagaggg gggacttttg cgataggctc ttagtcgttt ccgcggggag
       61 cccaggccta cttgggtaaa ttgctcactg tggaacgacg cggaagaaat gccccgaaaa
      121 gccatttcca ggaacttcca cccggcgagt caccttgccc attttgacag ccgctttttg
      181 gctttcccgg cgcaaaagct cgttggccaa agccgcggga attcggtggg ctgacctcgt
      241 gaacatgtgg gggatcaaac cgccacgccc ggcattcgat ctcatgaaat tgtatgacac
      301 gctccagccg ttgccacaaa tcccggttcc tgaccggcac ccgctctcca aatcgttccc
      361 aacaccaatc atgcaacggc caatcgcgaa ggccgtagag caacccctct ttcagtgctg
      421 ggctccgtgt ccaaataatg atccgcgagg attggttcag cgattccaat gcccgcagga
      481 ccgtcagcag ctcaagccgc tcaccctgga cacccggttc cacgtcctcg gcgattaatt
      541 catcgtgccc atcgggggag cggaggataa accgccagcg gtgggggtta ccctcaattc
      601 cgacttcgta caaaagagta aacgtgagtc gtccgccgct atcgggcgcg gccggatcca
      661 tcagaccaaa cgccgatttg tcccatcgag aaacactatt acccacagtg gtgcctcacc
      721 ttgagacgtt gatgcccaat ggaatgtgat gacgatagcc ctcaagagtg gggaggtcgc
      781 agcgttaccg gaacccattc caaaaaccac ccgacgacgg tgccgcaacc cctctttgcc
      841 ttgtatcgtc acagagcccc taaagtgtgc agtttatcca acaagaaaac tttaacagga
      901 ggcggcaagt aacctgcctg tgttggtccc cgcggtgcca gtcttagccc tgcttctgaa
      961 gattgtgcca ccagtcgcag ccgtaattgc caaccttgta ctgctcaatc tggccggacg
     1021 cggctgggcc ggcggccttc gcaagcagct ccttgacctc ctcttcgctc agtggttgga
     1081 aatcccgggc catggccagg tcttggcgca ggttttcccg cgattggatg ccgcaaatca
     1141 gacttgtgat tggcagcgac agggcgaaac ggcgcaagag cggggccggg atgcccagct
     1201 ctcgcggaat ccgcccgttc tgagacccca gcgctttcat cccaatcaca ccgatattgc
     1261 gtttcacgca ctctggcagg acctgtttct ggaagctctc ataatgggca tccaggacgt
     1321 tgatcggcat ttgcacgctt gcccactcat aaggtttctg gagcatcgcc agatggaacc
     1381 gtgggtgctt gtggccggtg aacccgatat agcggacttt tccttccttt tgggctttga
     1441 gggcatacgc gagggcgcca ttttctccaa aaatcaggtc cggatcatca tcccactgga
     1501 tgccatggaa ttgccacaga tcaatgtgat ccgtcctcag gcgacgcaaa ctgtcctcca
     1561 ggttttgctg ggctgtcttg gcatcccgac cgcagacttt cgtcatcaga aaaaccttat
     1621 cccgatactt cccttccgcc atggcctcgc ccatccattc ttcgctgcgg ccttgatggt
     1681 attcccagca gttatcgaaa aatgttagcc cgttatcgac cgcctcgtgc atgatggcca
     1741 gggcctcctt tttctcccgc acggccccaa catcccagcc ccccaggccg ataatcgtga
     1801 cccgttcacc cgtattgccc aatggacggg taggaagccc tccggcactg gtctcttgcg
     1861 acgcaaatgc catgcgaccg agggccagac ctgccagaac tgtctgcaga aactcgcggc
     1921 gagatgatgc gatcatgggc aagattcctt tacaaagcca accggtaacg ttctcttccc
     1981 caagggcttg ccaattttac gcacgggtgc ccaaagcgaa aagtcgttta ccctagaccg
     2041 ctcgtctcga aaagccggga actgctcacc cccaggatga gtgaactgct aagcgggtct
     2101 gctctccaca aggcctggac cttccgcggc ccggggctac aacatgacaa ccgatgtggc
     2161 gtcaacgacc ggaacggatc tgggcagggt taaactctgt tccattcgtc accggctgga
     2221 cagagtgcga aggatccagg tcatgacaac tctggcagtt catcttgacc atggcatccg
     2281 ccaattgcgc tttcccatga cagtcattgc acgctttggc cgttggggaa gtgggaagct
     2341 ctcggttagt ctgcatgtgc gactcgttca aaatggtcac caagaaccgc acaccatctg
     2401 cggtcagacg acggcagcgc tcggtttttt ctttgcaaaa ggcgggatag cccgagacct
     2461 tgcaccaggc cccaactgag aaatgacaaa gaatcgaccc ggcgacactc tttgggatct
     2521 ccatgctttg cggagcatcc ttgggcacat agacgggtaa ttccgaggat tcgtaccaat
     2581 aagcgaaggc ctcgatgagt ttctcgcgag tgttttgatc ttctggataa aacaagccga
     2641 tgagagcggc agccccgttg acaacaccac acaaagaccc ccattttccg acaccaccct
     2701 ccccgtaacg catcatgctg taaggaaatt ggctgaacgg ctctccgaac cgctctccta
     2761 gcgccgagat aaccgagccg aacatcgagt acatgcaacc gccgtccggg tacatcgcat
     2821 acgcccggtc ggctatctcc tcggcattga gaggcgcgta cttccaaccg tggtcggctt
     2881 cggaggaagc tcccaatcca ttgccgttgg ctgccggggt gaccggctcc gccgaaacag
     2941 gggccaccaa accttttgtg cacccagaag ccaaaatccc gcctgaaatc aaccccagtg
     3001 aagaaagcgc tacccgtcgc gttaatctct tctccatagc ttccccggta aaacagcgag
     3061 taccctgact tatcgcgaac aacctttaga acccaccttg ccttggcgtg gatgcacatt
     3121 ttaccaaata tgcatgaacg acgcaaggaa tgtcacagaa gggtgccgcc gatattttct
     3181 gccgggtctc cgcgacacgg cgcgagacaa gcagcatttc cgcggcccta tcgagagccc
     3241 atctccaagt tccctctccc tctgggagag ggttagggtg agggcacccg accgtcggag
     3301 tccaccgaga aaaccggcag cgattcgagc caccgtcact cctggcccct ctcccgcgga
     3361 acaggagaag ggaggttgta ggatactagg cagcaaagac accgagtcag gacatccaat
     3421 cccgccgggc attccttgca cgtccccgat ggcctcccaa gcctctggga gggacgcggt
     3481 cccccgtgtt cgtcgcgtct gggcaccgcg ggcgcaagtc cgaccggcgt caaaatcgtc
     3541 cggcggcaac attctctgcc gaaaacgttt gacgcgagcg ggcggggcaa agtttaatga
     3601 aaagatgcgg cgacgccagt ccacacgtca tcgccgacgc ttggtgaggc caagctttgg
     3661 gaggagcggc agtaaatgct tggattcgta catcccatca ttgccttaga gcagtccaag
     3721 tttcaaggcg gtctgctggt cctcaatcag tccggtgacc cggtggaatt ccgctgtacg
     3781 gtgcctgtga ggacaacccg gttacaacaa attctttggg ggcagcggct ggttgctcac
     3841 gtcttgggac agctgatcct tcggcctttg atccagtccg cgaagactca aattgccgtc
     3901 gttcttgtgg agagggagga gttccttcca gtgcgaggcg atttgtccgt tccgctctta
     3961 ttgatccgac gtcaagacca tccctccgat cggatccagt caccgaacac ggtaaccatc
     4021 gcccacgtgt cccccggcaa ggaagcctta ttgctgcaat ctgcgtgggg ttttgaagag
     4081 gacctcgaag tcggttgccg gctggtgagc gaattcctca aaacctgtca ccccctggag
     4141 gcctttcagc gaatagaaca agcactccac atcctgaagc cgaaacttga ggagcaggtg
     4201 agagtcacgg ggtaatcgct cgggcgccac gcggttggtt gagccggctc atctttaaag
     4261 cctatcccat catctcccct ggcgcggtcg gcgggagagg ggtcgcgggt gagggtggct
     4321 cgaatcgatg acgcttttct caatcgattt tgacgttcgc ttgccctcac cccagccctc
     4381 tcccagaggg agagggggac ttttttagga tacacccttg atatccgcga ccaacccggc
     4441 gtttgcaaca cctggacggc ggggacaccg tccctcccaa tttcgccccg ggaggatcat
     4501 cggccccgat gacccgggca ccgatcaaaa cgcacgccca ttttggcgat cagactcggg
     4561 cgcttgtttg cgttctgggg caggcacggg ggcctgcccc tacgggggtc gatggactca
     4621 taaacgggta ggggcaaccc cctgtggttg ccccgtttct tcgtagcccc agcgctttgc
     4681 gctccgaccc cggccgcatt gttggcattt gacgaggtca cgggcgttga tggaccgtgg
     4741 ggcaggcaca ggggcctgcc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nngtgcgggc aaccccctgt ggttgccttt ttctttttga gaaaccttaa cgattttgcc
     4921 gattattcaa aaggcgctga aacggctgcc aggcatgacc ttttcggctg gcaatgggga
     4981 ccgggttcca gctggcgccc atgtcggtgt cgcggaatca ctgtggtgcg agatcgagac
     5041 agcacgacag ggcaattgcg attgacagaa gaccgtttcc gctaggacaa tcgctctccc
     5101 tacgccccga cctaggggac aaccgtgggg gatactaaac cgacgatgtt tcttcacgag
     5161 atcgagaccc aagtgagtgc ccttccatgt ggaaagcagt gacgactacc cttaatcgtg
     5221 ccttgacctc cccattgctg tggggctcgg ccgcgtttgt cgcatttcag ttttttctca
     5281 aagccgacct gccgggtggt gattttctcc gccgctacac cgcgggtcac ccgattgagt
     5341 acatcgaaac atttcttttt tttctcgcac tagcggcctt gattcacaag ggactgacaa
     5401 tcctctacga gtggtccacc ctcgatcaac cgtggttatc cggtccgctt aacgccccgg
     5461 cggatcgtct cctcggcgat ctcgatgcgc ttcctgaggt cctccacacc agtcccgtgt
     5521 ttcgccaact aagggccggc ctgagttacc tctgccgggg tggcaccgcc caagaattgg
     5581 atcaacacct ggcctttgct gccgagcagg aggccgatga acgccgtcat cactacgcct
     5641 tcgtgcggtt ggtcatctgg gcgattccca ttttgggttt tctgggcaca gtggtgggga
     5701 tcacgctctc gctcggtaat ctcgcaccac aacagttaga ggagaccttg cccgcggtca
     5761 tggccggatt gatggtggct ttcgacacga ccgccctggc cctggccctg tcgatgatcc
     5821 tcatgctggt acaatacgtc gtggagcgct ctgagcagcg gctcctcgcc gctgccgggg
     5881 aaaaggtcga gaacctgctc ctttcccgct tccccgcaca gtcggtgccg ggacgggagt
     5941 tgaccgccgt gcgggtcatg ttggaagaag ttctgacgac ctgccgccac ctggtggaaa
     6001 cccaggtcac cctctggcag caaagcctgg aacaggccca aaagcagtgg caaggcacct
     6061 ggtccaattg gcaggaacaa ctggggaaca gacttgccac ggccctggaa aacgccctgc
     6121 agcgccatgc cgaccggctt cacgaggccg aggcccagct tttcgagcga cttcagcagc
     6181 gggaattgga agccgcccaa gacctgaccg cacggtggaa atcactgcta acagaggcct
     6241 cgcgtcagca ggaagaatgg gccagcctcc agaaaatcct tcagggacac gtgacggctt
     6301 ggcagacact tgcctccgag acccaacacc tcgctcggtt ggaggagttg ctccagcaga
     6361 acctaacggc catcggttct gccaaacact ttgaggaggc tttggcaagc ctggctgcgg
     6421 cagccaacct gttaagtgcc cacctccacc gtatcccctt ggaaccaccc cgggcggaac
     6481 gaccccgacg ggcagaagcg gcatgaactg gcgacgacgc aaacatagca aatcgggcga
     6541 aaaccccatt tcgctgttcc ccttcctggc cgtgctcatc tgcacgatgg gggtccttat
     6601 cctgctattg gtcatcctcg ttcgccaagc ccgcctaaag gccctcgccc aggcacaagc
     6661 ctcatcaaat cctgcgtccc agaaagaggt aagctcccca attcccacgg aagacgagct
     6721 taagcgctgg gaagaagatt tggcctggca ggaagaacaa ctgcgaacag cccgggaaaa
     6781 gacgcaggcc caacttaccg aagcccgcct gatcttggga cacattgagg attccatccg
     6841 tacctttcgc caggagcttc agagcttgca ggagcggctc cagcggctga atgaagtcac
     6901 taacgagagc ggaagcgtat caattgccga acttgaaaac cacctccacg aactgcacga
     6961 agccgcacaa agtctcaagg cacaagtcga agccaaagag caccagcggc gtcgcaaaag
     7021 ttacgccatc attccctatc agggcaaatt cggcaccgcc cgtcgcccca tttacctgga
     7081 atgccgcggc gacggcatcc tcctgcaacc ggaaggaatt cgctttacgg tgcaagattt
     7141 tctcgggcca ctagggcccg gcaacccgct ggacgcggcc ctgcgggcga tccgagaaga
     7201 actcctcgcc cgaaaggaat tcgaccccca agccgatggc gagccttatc ctcttttgat
     7261 cgtgcggccg gacggcatcg aggcctacta cgcggcacgt gccgccatgt cttcttgggg
     7321 aacggaattc ggctacgaac tggtggagaa ggactgggag ttggtctatc cccaacccga
     7381 tccccgactg gctgaggtcg ttcaacaagc tgtcgatctt gcccgcaaaa gacaagaatt
     7441 cctcgtcaag gcggcgcccg gaaaatatgg gcgaacacct cggcagtatc gcgcagcgcc
     7501 gtatgttggc ggggctgtcc ccaccgaagc accagccaca ccgcccgaag accccatgct
     7561 tgcccaatgg ctagcccggc aacagtccgg taacaccgcg gtgcgtccag aatccgcgcc
     7621 cggcggcgcg gccgcaacct cacggacggg caactcgacg tcggagcaag cctccaccca
     7681 gtcgcctgtc gagcgccaag tcgaggacca cgtcaccatc tccgaccttc gtgtgatgtc
     7741 gccacaccgg cctgtcggtg atcagccgca ggtcggcggt gccccctcac ccaaggaatc
     7801 aaccgctgtg gagatcacga cgggcgtcac tctacgcccg ttggcggaac aacggggccg
     7861 aaactggggc cttccccccc atgccgcttc ggccatcccg gtgacccgtc ccatccgcat
     7921 tgactgtttc cccgaccgag ttgtccttat cccggaacaa ggtctcgacc atatccgagt
     7981 cttctacctg accccgtctc cggcaaaagc ggtggatgaa ctagttaagg ccattcggga
     8041 gtacgtggaa acatggggta tcgctggcaa caacatgtac tggcgaccgg tcctttccgt
     8101 ccgtgtcgcc ccaggggccg aggcccaatt ccaaatactg caaatccttt taaaagacag
     8161 tgggctaacc gtggagcaaa ccagataacg atcgagcgag cacgattgtg cgatcaatga
     8221 atatggcgta ttgtccagga aaactttttc cacgatgaaa aacacggaac gagacggaca
     8281 gatcaacgcc ggctacgatt cctttctcga caccgtcacc aacattgtcg ggattctgat
     8341 tattttggtg atggtcgtcg gcgtccgcgc gcagcacctg cccttcaccc tgaagccgga
     8401 cacgtcccac gcagaatccg ccgaagaaca ggcccggcgg gcccgcgccg aagagcttgc
     8461 ccggcgatcg acggcccttc tttccctccg gcgcgatatc cttgaacttt cggcaactgt
     8521 cgaaatggtg gaggcagaac ggctcgcccg cgaacgtgag cgccagcgac tggccctggc
     8581 cgccgccctt ttgaaaaagg aactggacga aaagactgtc gccctctcgc aggaagagcg
     8641 acgccgtgaa caattggctg aggccatcgc tgcatgtgag cgagatatcg ccgcatccca
     8701 acagcgcctt gccgagttgt cctccacaac cgaacaggtg cagattgtca atcaccccac
     8761 gccgctgagc gtccccgtcg atttcgagga gattcacttc gtcatacggc agggaaaaat
     8821 cgcgtacatt ccgctctcat cgctcctcga tgagctgatg tcggacgccc gccgaaaaat
     8881 cccccgcctc ttcgaggtcc ccgaaatcac cgggaccgtg ggaccggtcg gcggattcaa
     8941 actggagtac tccatcgtcc gtcggaccat cggcgaggcc gagatcgccg gtgttcccgg
     9001 tgccggggca tacgcccaat tgcgacgctg gattctcatc ccggaaggca acgtcggtga
     9061 acccgtcgcc caagccctag aagaatcatc cgaattccgt cggcgtatcg ccggatatcg
     9121 tccggacagg acgaccgtca ccatctggtg ctatcccgac ggcttcgccg acttccgccg
     9181 tctcaaagat tttctccacg agcggggcta ttccgtcgca ggccggcccc tgcccgacgg
     9241 cttccccatc acaggctcgc ccggcgggac caaatcctcc gcacaatagg tggtccgaag
     9301 ccattccgga taactgcaat aatcgttacg attccactcc gggacgtggg ccaccggcgg
     9361 tcaaccggtg ggattccccg tcgttcaggc tttgcgcttg acgaattcgg gccttttatt
     9421 tttcctagat agtgatccaa ttggcgaagt gcctcaagtc aatcctatgc ggagggtctc
     9481 atggcgccgg ctgtcaaacc gatcccgctc aatcgggcga tcgccccttt ctttgttggt
     9541 gtggatttag gcggcaccag caccaagatc ggcctggtcg atgacctggg tcgtccggtg
     9601 tgccagcccc aggagtttcc caccgctgtc gaagatggtc cggaacagct tgtcgcccgt
     9661 gtggccgagg tcatcaacca gatgatccag acctccggac tggaatccac agaaatcgct
     9721 cgtgtcggac taggttgccc gggtattttg gaccttcaca caggcgtgat ggtcaacccc
     9781 accaatttcc ccggttgggg tggatttccc atccgcgagg cccttgctga tcgttcgggg
     9841 atgcccgtcg tcatggccaa cgatgcctcc gccgctgcgt acggcgaact gtggatcggc
     9901 tctggccgcg gcttccgcag cctcgtcctc ctgacgctgg ggactggcgt cggctgcggt
     9961 gctattatcg gcgatgtcat cattgagggt gaacacggcc acggtacgga atgcggtcat
    10021 atcctgatcg atccctccga cacagccccc gtctgttcct gcggccaacg gggacacctc
    10081 gaggccttcg ccagtgccac ggccgtcgtt cgccgtgcac gagaaaaaat tgccgccgga
    10141 gaggctcccc tccttgccga aaaggtgtcc aacggcgaaa aactgtcgcc ccttttgctg
    10201 gccaagttgg ccgaggcagg cgaccaaccg tcgctcgatc tgatccttga aaccgcccgc
    10261 tacctcggct tggggatcgt cacgctcctc cacacatttg acccatcagg cctcctcttg
    10321 ggtggtgcga tgacgtttgg tggcgacaag tctcccgtcg gacgccgctt cctggaacgc
    10381 attcgggaag aagttcgccg ccgggccttt gccgccctgg caaacaaggt tgccatccgc
    10441 ttcgccgcgc tgggcggaga cgccggcttc atcggggcgg ccggcatcgc ccgattgcac
    10501 caccgacgtt accgatgaat catccccaag gattactgca attcgacacg cgggtcgaga
    10561 atggtgtagg ccaaatcgac caggacgttg gccccataaa gcaacagcgt aaagaggaga
    10621 accaatccca tagaaagcgt atagtctcgc tgaagagccg cttccacaaa atgcatcccc
    10681 aacccaggga tggcaaagat cttctccacc accggcgaac cggtgataat tccagccaca
    10741 gccggcccga ggaaagagac caccggtgcc aacgcccccg gcagggcatg cttaaaaatg
    10801 atcgtcacct gattcagccc cttggcccgc gctgtgcgga tgtaatcctg cgtgaggacc
    10861 tccaatagtc ccgtgcgcgt caagcgggct atcgacgcgg catagggcaa gcccaagcac
    10921 acggcgggca acaccaagtg cgacggtcgg ccccaacccg ccgccggaaa acacggtagc
    10981 acaaacacca aaagcatgat gagcacgccc gccaaaatga aattgggaac ggccagtccc
    11041 accgtggcta agaccatcag gccacgatcc aggacactat tgcgaaaggc cgctgaggca
    11101 acccccgccg cgaaacccaa tgtcagcgcc aatcccatgg ccgctaaccc taaggccgcc
    11161 gaaataggta acccttgggc aatgacttcg ttgaccgtga agtccgccat gcggtagcta
    11221 tagcccaagt cgaattgcac gacgtggcca agttctcgca aatactgctg ccaaaccggc
    11281 tcatccaaat ggtagcgcgc cgccaaattc ttttcgattt gcggatccag acgacgttcc
    11341 cgatccaacg gtccccccgg cactgcccgc atcagaaaaa agcttacggt gaagaccacc
    11401 cacagcgtga tggccaacca gatcaagcga tcaatcaaaa accggagtaa catcctcggt
    11461 tcaccccaat gcctcaggcg ccgacagacg acaggttgtg gttcccttaa ctacttccta
    11521 ttgtcctgcg ctgccgctag caatcaacgg caaacccgac attgagagag aatctgctgc
    11581 ggtgtggcct gatagccgct gtagaacggt ccgaactcag cgtaacgggc gctggcctcg
    11641 tcaaaccgca tgcgatagac gatatccttc aaaaactgtg gattcctggc ccaaagcgtt
    11701 actccccatt cccagtcgtc caacccgatg cccaccgtca ccaattgggt cacccgtccg
    11761 gcaaacttca aacccgtctc gccgtgctcc cgcattaggc gatcgcgctg ctcaaaatcc
    11821 agcaggaacc aattagcctc cggcaagcga cgcttgttca tcggataaaa acaaaaggca
    11881 ggccagttgg gtaaatccgg ttgaagccgc tgccggcgca taatctcata acgccgcgca
    11941 tactgccgcg ttcgttcctg gtgttccggc gagtcaggcg gcaaaccttc ctgctgcaat
    12001 cgtgccgcat attcctccac tgtgggaacg tactctgaga cctccgtcat cgaaacaaac
    12061 gaccacatcg gttcaatggt gggtcccagc aaacccgcca aaagccgctg atgaacgcga
    12121 tccaacttga gcggatcggg gtcgagcatc aagatcccga aatcggcttt atggcccatg
    12181 ataacaaagg tctgaagtcg catgggggcc tccggggaac gcggatcgag cgtctcacaa
    12241 aagtccttgc acccgtcggt gtactctccg ggggccaatg tacgaagtcg catgcgatca
    12301 aaacggtaaa accaatgctg acagtgccaa ccttcctcag gttgcaagga gacctctggc
    12361 cgcggtgacg tatcgttgtc agacgccatg aaccaacctc tcgaacgtgt ccaaccatcg
    12421 gtcaaccatc ccgcccggga cccccgcacc ccaacacact tcgcaaaagg attacccgaa
    12481 ccaggaattg ttccctcccc tgccccgaaa gacaagtcaa cgactctcaa gccggccgac
    12541 ctgccccaac gcaagctgga agggaatggc cggccgcgcc gccgtgacgg taaatcttgc
    12601 tcaataccga acgtcgcaac atcaagaaac accccgcact ccaaaattgc ccacccagca
    12661 ctccttttgt gacatcaata gtacatattc atcacaatgt gattcactgt ccttcttgaa
    12721 aatagtccac ttcgtgtgtt atactgccct ccgttgtggc tttgacaaac ttcgattgta
    12781 agggcagagc gtgggcagct cgtccatttc aggccctccg aaaaccccac cgggacgaga
    12841 gcccctttgt gcgctgcccg gcaatgttca cgacccaagc gcggactgac gtccgttttt
    12901 ttggggccct tgtagtgttc atatgtacat taccaagcga cgcatgcacc acgcggaggt
    12961 tacccgtgct caaccgtcac ctcgccgata agattctcag actcctcacg gaagaaagac
    13021 ttagtcagcg ccaggtggcc cgattgacag gcgtaagtcg tgccaccatt gccgccatcg
    13081 ccaagggaga gtggcatgcc cggtttcggg agcgtcccaa ggacgaaagt gatcaaccgc
    13141 ccgctcccag ccgctgtccg gagtgcggtg cccttgttac accgccgtgc ctggcttgcc
    13201 gcatccgtgc catgaggata cggggtgaga ttcttcctct gccgggcgaa ggtcccggtc
    13261 ccctaaagct cgaactccgc ggtccgcact atgcccgcta tctagaagtc cgcgctgccg
    13321 ccatggctcg gggtgaagga gaacccctcg aagaccccac cgaggatgcc gccgatgatg
    13381 acgcctggtc cgacgaggcc ttttgggcag atgag
//
LOCUS       DAOSUT010000115        13264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2775_length_13264_cov_8.152245, whole genome shotgun sequence.
ACCESSION   DAOSUT010000115 DAOSUT010000000
VERSION     DAOSUT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_13264_cov_8.152245"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1528
                     /locus_tag="PLD05_10935"
     CDS             449..1528
                     /locus_tag="PLD05_10935"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP77999.1"
                     /translation="MRNKVRLRVGFTLVELLVVIAIIGILIALLLPAVQAAREAARRS
                     QCTNNLKQIALAVHNYIDVYKVFPALGVQTCRGRYYAWSMMILPYLEQKPLYDAIMAQ
                     AQSPDMLPEPWHTTQNVPSSPASWAPFVRQWWQIDIRTYVCPSDQPIPNRQESPARLN
                     YKGCVGDDYHQNHFRPDQNNRQNRGIFQCERYVGLEMVTDGTSNTIMLGEMVAGGYPD
                     DIKGGVALNMTAWNPAACLARVDPATKKLIPPVRADFRPVGGRAWDGRPYFTGFATLV
                     APNGPSCHWGDVDGNEHMGTASSFHPGGVNVAMADGAVKFISETIDVGNQAVDDVDTP
                     GNRRSPWGVWGALGSAYGGESVTAP"
     gene            1671..2135
                     /locus_tag="PLD05_10940"
     CDS             1671..2135
                     /locus_tag="PLD05_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOP78000.1"
                     /translation="MSWRLGFAGLLILLLAIAGCGSKGPKLARVRGKVTLDGQALPNA
                     LVTFMPEGGGVASSGTTNANGEYELVCPQGRGAVVGKHKVSVRTLPPQQSQAQEVSSD
                     DPSYLAQYSGQSSATAQPFVEKIPPRYNTQTELVKEVKPGSNEINLDLTTSP"
     gene            complement(2540..4573)
                     /locus_tag="PLD05_10945"
     CDS             complement(2540..4573)
                     /locus_tag="PLD05_10945"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOP78001.1"
                     /translation="MHRKPATIFWALTVGTMLWLMQTLPVPSEERPPWLGNRAGISDE
                     LLAPWTPVEVKDTTISVWGRSYSFGPLPLPAEVTTAGAEILAGPVTLHCTADGQPITW
                     SAGGRSIVEHFPTRAILRFSAQGGPLRCEGEVTTEFDGMIRCGLRLVPVADKITIENL
                     ILEIPFKSKHAVFMHSWPGRWGSAANSHAVPPVEWQSAFKPFVWIGDYERGLAWFCES
                     DENFFPASLNRVIRVRRDGEITHQRVELIGKAQTVSQPLRYVFGFQATPVKPLQPDAW
                     DYRIVHVGRYGLEKQMFSPPGQAPKLLLDYLAEAGVKTICFHEHWSNIQNYPEAARPE
                     ELHSLVEACHKRNIQLLLYFGYEMSNIAPEWEKYAEECLVAPRAGGYKRQPEQTAYIV
                     CYRSHWQDFLAAGIERIIREFDIDGVYLDGTSEPWGCTNTQHGCGYTRPDGSIGPTYP
                     FYDTRQMMKRIYTIVKHHNPAGQVNVHQSTCMVIPTLAFATSYWDGEQLQSVARPDSP
                     TKLLPLEAFCAEFMGRNWGVPGELLWYANGPFKWEEAVSMALLHDIPVRPNVDRLELF
                     RRLWRLFDEFDRHASQWIPYWQSSGVVACSHPEIKVSLYNRPGNGCILVAVNVGTEEI
                     QADLTLGLSALGQSDLTIWDVLTDKEIGPLGEKMPLKLGPWQHAIFWLKPRRS"
     gene            5186..7495
                     /locus_tag="PLD05_10950"
     CDS             5186..7495
                     /locus_tag="PLD05_10950"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOP78002.1"
                     /translation="MRNQFPTASRAKHWASVTSLVWNLVGVGCLLMLLADRGQVPKAV
                     GEEHQQSPRPGLQVMVEPNTERLTAYQIFELTFRHSGHYQNPTWDVSIEVILRSPTGK
                     QYRVGGFYYGPAEEETADRTRDGGKSLEANLPNESRGPLQVWKARYAPSEFGQWSYEY
                     RFRNPFGEEAVGTGTFHVVRTRASFPGWLRISKDNPYRFVFEDGSPFFPVGFQDCEGD
                     HNKNGTTLDSKSMEGPFRLDKATERPQPPPGSLFARGPSMNPVNGDVYFGKHARAGFN
                     IFRFSPHNCSISVFDLDRVDYREAKMVDQLLEMVRKYRIRIYYGIFGFAKVFNDQPDN
                     AEGMRKVRRLIKYSVDRWGAYVDIWELLNEQHASEMWYAQIIPYLKSIDPYQKPVATN
                     WERPQLEGIDINAPHWYQNENELDSDLVTAQRIARDKRFGKPVVYGEQGNSRGQQDRT
                     AEGIGGVWDPGSARRMRVRLWTALFNEAALIFWETSYAKDGHYMNLWIGPEERQYVKA
                     LQTFSAGLEGNVRPREVLLSGRDAKDVRGYGLASPSCQAVYLHHFACQQCKDEGKPPA
                     QHRWDHKRGTVSDLAVELESFKTLSAYWYCPQTGKILQRFRISAGRREYAVPPFEVDI
                     ALLATEKPAPDSDRDDLANDEDPDDDNDGVPDDQDAFPLEREEWADVDGDRIGDNFDA
                     DIDGDGVGDDRNGNGIPDYQEMDADGDGVPKANAIPWDAFPHDPHEWRDTDGDGIGDN
                     ADDDDDADGFTDQEERKARTDPLNPLSFP"
     gene            complement(7684..9708)
                     /locus_tag="PLD05_10955"
     CDS             complement(7684..9708)
                     /locus_tag="PLD05_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78003.1"
                     /translation="MLGINLVLGMVIGFQIDVPAQERLQELVQRAGNTDDDEERLELL
                     RQIRALPGVDPDLAEHAEKLIRFVEQWVEGKNLHFYGGEVNQNRDIDFGIPATSPLYP
                     LTYLYRGRAVLSVVMENGNLWSYGDRRREWFGIARQFFERAHQAFPQNRIARMYLGEG
                     IPWTRNWEAVPEAPEWAVHQRVALEGLADIIEWWLDHRQQPDGAFGGGWGDDCEMWRF
                     WVPGLIGFDDPKICAGQKRFSEALFQQPHMAAGYMSRMTDVEHSAEDSTDTILPMMLL
                     EPDNLVWQQRVLRLAELMENLWTGRNERGFRQFKSTYFTVDRVHPDPQRACDTPYHVR
                     AVQPVFLYWLRTGDQRVGRVLCEWTDTWVDAAARSENGKPAGVLPATIHWPDGSIGGL
                     SPHWWDPRNHSEPTLYQWPSAMGYLTDALLLTFHMTGDEKYLRPIHSMAELALRYSGR
                     NLPKDIEPGSEAWCALQMGFLRQTLAKYRFLTGDGTWDRLLEGRQAPWLHYALTGDEQ
                     PLVAALADTATAFSYNFPGYTSEVRYTDRVLRLPAVFQGDVVLAEPKFPVQSPDTALL
                     YSAVTGDPGGLGTFPLNAVRWLTRPREIAVLVRMAAPDGLEATLFHFGRSPRAMGAEL
                     NMLKPGDYTLKIIARDHVLKEEAFTVSGPRTRIAFEIPSRQSCRLVIRRN"
     gene            complement(9776..10921)
                     /locus_tag="PLD05_10960"
     CDS             complement(9776..10921)
                     /locus_tag="PLD05_10960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOP78004.1"
                     /translation="MLRLVFAGCLLWCWVGFSAVGAESDYFPPLESQGGWRKLDSPAD
                     IRRLAGMDPQKLEVLKEWLLVSDQRPFAAVVIRRGYIVLEVERGNSSKTDTRNIKSCA
                     KAICATVLAIASEESLAGRTPKKMTFDDPAFSFIPWAEPLSDPRKKQILVRQLLNHTS
                     GIAPEYTGAKNRGPWDYVLGHSGDPVTAKLAFDPGTDLGYSTHAFYHAALVCEDVTGV
                     PYDQFAIEKLFKPLGIERWWFAYLDGTIGRHPSHTLGLPARESARICYCMLRGGRWGD
                     RQVIPKWFVEETAAPTHSIHGIKCFNRDAESFSHGWELPARLTDGRGEGIPADARFKP
                     GSGGQLIAFVPSLDLVITRQTGGSGQWAYEEYLRRACQAVISVEAGE"
     gene            11168..>11469
                     /locus_tag="PLD05_10965"
     CDS             11168..>11469
                     /locus_tag="PLD05_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78005.1"
                     /translation="MKRRGLLLGLVSWVGALVFVGAMGLFGPRADAQQGPPPHALARV
                     IAVQERHTERLMSLPDVVGTAVGLDEMGRPVVKVYVAHAGVRGIPSVIDGEKVEVEV"
     assembly_gap    11470..11569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11570..12393
                     /locus_tag="PLD05_10970"
     CDS             <11570..12393
                     /locus_tag="PLD05_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78006.1"
                     /translation="GVRGIPSVIDGEKVEVEVTGEIYALGALAALEKAAKPSAVKAVN
                     PRGWFDRPVPIGVSTGNANEGSAGTIACRVTDGTNVYALSCNHVYARTNNASLDEAIW
                     QPGPYDVVTSSLINSDLDFVIGTLAAFVPIDFSSSGKNYVDAAIALVSTDTVGTSTPS
                     GGYGTPSSETSTAYVGMRVQKYGRTTRLTYGTISGVNATVIVNYGIGQAKFVGQISVK
                     GSRFIQPGDSGSLLVTRTGNKPVGLLFAGNSSGSFAIANPIDLVLDEFNVSIDGQ"
     gene            complement(12981..>13264)
                     /locus_tag="PLD05_10975"
     CDS             complement(12981..>13264)
                     /locus_tag="PLD05_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78007.1"
                     /translation="GSASGWTPTGTGIPTSGGCQPGALAWEAYKLPSPFGRGVGGEGA
                     PVGSATWTGGAGSVIWAPVGFARVRFLLANRPAFSPRGKEAVGLTTAPP"
BASE COUNT         2894 a         3642 c         3735 g         2893 t
ORIGIN      
        1 gttcgaatcc tctccagggc aatttttccg gaccggcgtg gcggggacat gttacacgct
       61 attcatcggg catcgacgcc gggttcgcga aagattcttc gcagtgtcgc ggttcggtcg
      121 tgccaaaccc tttggcgttt gcctctaacc cacggcccgg agcattttcc caggggggct
      181 aaacttttgt tggggagctt atctttcggt gtgtcatttg cgccgcgctt cgcgcaaatc
      241 tggtcagcat cagtccgttg ataccacggc gctctcgttt ttgagatttc cgaaccgggt
      301 ttcttggtca accgacaatt tgcttgcaaa gaaaagtagt tcggcataaa atcgttcgcg
      361 ttgggttggt gattgtaatg gttattggcg ttttctagcg ccaggagtga tgtttgttgg
      421 gttctgtaat gggttggagg gttatgagat gaggaacaaa gtaaggttac gtgtgggctt
      481 tactttggtc gagttgctgg tggtgatcgc tatcattggt attctgatag cgcttttgct
      541 tccggctgtt caagctgccc gtgaggctgc ccgacggagc cagtgtacca acaacctgaa
      601 gcagattgca ctggcagttc acaattacat tgacgtttac aaagtctttc cggcgctggg
      661 tgtgcagaca tgccgtggcc gttactatgc gtggtcgatg atgattcttc cttatctcga
      721 gcaaaaaccg ctttacgatg ctatcatggc gcaagctcag tcgccggaca tgttgcccga
      781 gccgtggcac accacgcaga acgtcccttc cagccccgcc tcctgggcgc cttttgtgag
      841 gcagtggtgg caaatagaca tccggactta tgtgtgtccg tccgatcaac cgataccgaa
      901 ccgacaggag agcccggctc gcctgaacta caaggggtgt gtgggggatg actaccacca
      961 aaaccacttt cggcctgacc agaacaaccg ccaaaatcgt ggtatttttc agtgcgagcg
     1021 ttatgtaggg ttggaaatgg tcaccgacgg tacctccaac accatcatgc ttggggaaat
     1081 ggtggcggga ggatatcccg atgacatcaa aggtggtgtg gccttgaata tgactgcctg
     1141 gaacccagcg gcctgtcttg cccgtgtgga tccggcaacc aaaaaactaa ttcctccggt
     1201 tcgtgcagac ttccggcccg ttggtggacg agcatgggat ggtcggccat actttaccgg
     1261 ttttgcaacc cttgtagcgc cgaacggacc tagttgccat tggggtgacg tcgatgggaa
     1321 cgagcacatg ggtacagcct caagtttcca tcccggtggt gtcaatgttg ccatggccga
     1381 tggtgctgtc aaattcatta gcgaaacgat tgacgtgggt aaccaggcgg tcgacgatgt
     1441 tgacacgccc ggtaatcgcc gatctccttg gggtgtgtgg ggagccctgg ggagtgccta
     1501 cggtggcgaa tcggtgaccg caccgtgata ggggtggtta gggcgagcgt tttaccaaca
     1561 actgttcgag gtggaggcaa agcatttcgc tccaccgttg cgttttgatg tttaaccgtc
     1621 acacgattcc agttgatatt gaaccgttac acattccagg agcagctgat atgagctggc
     1681 gtttgggatt tgcggggtta ttgattcttt tacttgcgat agcaggttgt ggcagtaagg
     1741 gaccgaaact ggcacgagtc cgcggcaagg tgacgctaga cggtcaagcg ctgcccaacg
     1801 ctttggtgac gttcatgccg gaagggggcg gggtggcgtc ctccggtacg accaatgcca
     1861 atggcgagta tgaacttgtc tgccctcagg gacgtggggc tgttgtgggc aaacataaag
     1921 tatccgtccg gacacttccc ccgcaacaga gccaagctca ggaggtgtcg tcggacgatc
     1981 catcctattt ggcacagtac agtggccaga gcagcgcgac ggcccaaccg ttcgtggaga
     2041 aaatcccacc acggtacaac acgcagacgg aactggtgaa ggaggtcaaa cccggaagca
     2101 atgagatcaa cctcgatctg accacttcgc cctgagtggc tgttattttc aggatgcgca
     2161 atagtgtgcg caagaaggcc gcgcgccact gagaacgtcc tcggcctgat caggtcagga
     2221 gcggttaaga cgtgccttca gacgaggggg tagctctttt cccacccggt ggtcgccgaa
     2281 agaggtgaga tgagatttcg agaagctggc cgtacgtccg gggtgccaag gtgggggtgg
     2341 agctgccgga ctgcgctatt cacaaatcgg ccatcttaac gatggcaggt caggcaagtg
     2401 gcgtggttgg cctagccagc gcgagccttc ctgggcggaa atacgcatac gaaaagagcg
     2461 aggaccgcga gacatttcct attccggcca ggaccataac caaagtcaga tggccttgat
     2521 ccgcggaggg atgtggcctt taggagcggc gtggcttcaa ccagaaaatg gcgtgctgcc
     2581 acggtcccag tttgaggggc attttctctc caagcggccc aatttccttg tccgttagca
     2641 catcccagat ggtcaagtcg ctttggccaa gcgccgacaa tcccaacgtt aggtcggctt
     2701 gaatctcttc tgtgccgacg ttgacggcca caagtatgca accgttcccg ggccggttgt
     2761 agaggctaac cttaatttca ggatgagaac aggcgacaac gccagagctc tgccagtaag
     2821 gaatccattg acttgcgtgc cgatcgaact cgtcaaagag cctccacaag cgtcggaaaa
     2881 gctcaaggcg gtcgacattc ggtcgcacag ggatatcgtg caaaagggcc atagacaccg
     2941 cttcttccca cttgaacggc ccgttggcgt accacaagag ttcgcccgga acgccccagt
     3001 ttctccccat gaactccgcg cagaatgctt ctaacgggag gagcttggta ggcgaatcgg
     3061 gccgggccac gctttgcaat tgttcaccgt cccaatagct tgtagcaaag gccaaggtag
     3121 gaataaccat gcaggtggat tggtgcacgt ttacttgccc ggcgggattg tgatgtttga
     3181 caatggtgta gatccgcttc atcatttgcc gtgtgtcata aaacggataa gttggcccaa
     3241 ttgagccgtc cgggcgagtg taaccgcacc cgtgttgggt attggtgcac ccccatggtt
     3301 cagatgtccc atcgagatac acaccatcga tgtcgaattc ccggatgatc cgctcgatac
     3361 cggcagcgag aaagtcctgc cagtgactgc ggtaacaaac aatgtacgcg gtttgttcgg
     3421 gttgtcgctt gtaaccgcct gcccgtggag caaccaggca ctcttcggcg tacttttccc
     3481 attctggggc aatgttactc atttcatacc cgaagtacaa aagcaactga atattgcgct
     3541 tgtggcaggc ctccacaagg ctatgcaact cttcgggacg tgccgcctcg ggataattct
     3601 ggatgttgct ccagtgttca tgaaaacaga tcgtcttgac gcccgcctcg gccaggtaat
     3661 ccaagagcag cttcggggct tgtcctgggg gagaaaacat ctgtttttcc agaccatatc
     3721 ggccaacatg gacgatacgg tagtcccaag cgtcgggttg tagcggctta acgggcgtag
     3781 cctggaagcc gaagacatat cgcaggggtt ggctgacggt ttgtgcttta ccgatgagtt
     3841 ccactcgctg gtgggtaatt tctccgtcgc gacggactcg aatcacgcgg ttcaggctag
     3901 cgggaaagaa gttttcgtcg gattcgcaga accacgccaa tccccgttcg tagtcaccga
     3961 tccacacaaa gggtttaaaa gcgctctgcc actcgacagg cgggacggcg tgtgagttgg
     4021 ccgcacttcc ccaccgtccc ggccagctgt gcatgaaaac cgcatgcttg ctcttaaagg
     4081 gaatttcaag gatcagattt tcaatggtga tcttgtcggc aaccggaacg agacgcagac
     4141 cacagcggat catgccgtca aattccgtgg tgacctcccc ctcgcagcga aggggacccc
     4201 cttgagcaga gaaccgcaat atggcacgtg tggggaaatg ttccactatc gaccgcccac
     4261 cagccgacca agtgatcggt tgaccgtcag cggtgcaatg cagggtcacc ggtcccgcca
     4321 ggatctcggc cccggctgtg gtcacctccg ccggtagcgg taatggtccg aagctataac
     4381 tacgtcccca taccgagatg gtcgtgtcct taacttcaac gggagtccat ggggcgagca
     4441 gttcgtcgct gatcccagcc ctatttccta gccagggtgg tcgctcctcg gatggaacag
     4501 gcaaagtctg catcaaccac agcatagtcc ccacggttaa tgcccaaaag atggtggccg
     4561 gttttcgatg catggttttc tctcctcagg atcggactcg tcgcttcact tttatcccac
     4621 acgtgccgta ttccactgcg agtaagaatt gaccattgtg ggcgagcggt tctcctccgg
     4681 atcgccgata agtctttctc ccagtcttcc cacagcagtt tccccacact agtgtaaggg
     4741 ccaattgccc acgccaaaag aaggacgtga aagcgacggc agaaccaatg agacagaacc
     4801 tcttcgctga gctatgaagg tttggacgaa cacgcttgtt ccagtgccgg tacggccgat
     4861 accgcatccc gaggttgtca ccctactttt agaacctatc ccaaaaagcc ccccctccct
     4921 ccgggagagg gttagggtga gggcaagcga acgtcggaat caattcagaa aaacggcatc
     4981 gattcgggcc gccctcaccc ccgacccctt tcccgccgaa ggcttcaggg gagatcatgg
     5041 gataggctct tttagcgcac cgcattgcgc gaaagacttc cggccaggca cggcttgaaa
     5101 gggagagtac taccatggta gtatgtcggc gccggcggag agaaaacgag ttttgtcgga
     5161 gtcggttcaa tatggaggac aatccatgag aaatcaattt cccactgcga gtcgtgccaa
     5221 gcattgggct agcgtcacct cactagtttg gaatcttgtc ggtgtcggat gcctcctaat
     5281 gctgttagca gaccgggggc aggtgccaaa ggccgtcggt gaggagcacc aacaatcgcc
     5341 gcgccctggc ttgcaggtga tggtcgaacc aaacacggaa aggcttaccg cctaccagat
     5401 attcgagcta acgttccgac attcaggtca ttaccaaaac cccacctggg acgtttctat
     5461 cgaggttatt ctccggtcac caacgggcaa acaatatcgg gtgggcggtt tctattatgg
     5521 cccggcagaa gaagagaccg ctgaccgcac ccgagacggg ggcaaatctt tggaagcgaa
     5581 cttgcccaac gagtcgcgtg gcccactcca ggtttggaag gctcgctacg caccgagcga
     5641 attcggccag tggtcctacg agtatcggtt tcgcaatcct ttcggggagg aggccgtggg
     5701 gacgggaact ttccacgtgg ttcggacacg ggcgtctttc cccggctggt tgcgaatcag
     5761 taaggacaac ccttaccggt tcgtgttcga ggacgggtcg ccgttctttc ctgtgggttt
     5821 ccaggactgc gaaggagacc acaacaaaaa cggtaccact cttgatagca aaagcatgga
     5881 agggcctttc cgcttggata aggccacgga acgccctcag cctccgccgg ggtcactgtt
     5941 cgcaaggggc ccctcgatga atcctgtcaa cggtgatgtg tactttggca agcacgctcg
     6001 ggctggattc aatatcttcc ggttttcgcc ccacaattgt tccatcagcg tctttgattt
     6061 ggaccgtgtt gattatcgtg aagccaaaat ggtggaccag cttttggaga tggtccgcaa
     6121 gtatcgtatc cggatctact acggcatctt cggttttgca aaggtcttca acgatcaacc
     6181 cgacaatgcc gaggggatga gaaaagttcg ccggttgatt aagtactcgg tggaccgttg
     6241 gggggcatac gttgatattt gggaactcct taacgaacaa catgcctccg aaatgtggta
     6301 cgcccagata attccctatt tgaaatcgat cgacccatat caaaaaccgg tggccacaaa
     6361 ttgggagcgc ccacaattgg agggaatcga tatcaatgcg ccacattggt atcagaacga
     6421 aaacgagctt gactcggacc tggtcactgc ccaacgcatt gctcgtgaca aacgctttgg
     6481 caaaccagtc gtttacggtg aacagggtaa tagccgaggc caacaggata gaacagccga
     6541 ggggatcggt ggggtctggg acccagggtc agcccggcgg atgcgcgtcc gtttatggac
     6601 ggcccttttc aacgaagctg cgctcatctt ttgggaaact tcttacgcaa aagacgggca
     6661 ttacatgaat ctctggattg gtcccgaaga aaggcagtac gtcaaggccc tgcaaacctt
     6721 ttccgcgggg ctggagggga atgtccggcc gcgtgaggtc ttgctgagtg ggcgcgacgc
     6781 taaggatgtg cgagggtatg gtttggcctc gccgagttgc caggcggttt acttgcatca
     6841 ctttgcctgt cagcaatgta aagatgaagg gaagccgcct gctcagcacc gttgggacca
     6901 caagcgaggg acggtaagtg atttggccgt agagttagag tcgttcaaga ctctgtccgc
     6961 gtattggtat tgtccccaga caggaaagat acttcagcgc ttcagaattt cagcagggag
     7021 aagagaatat gcggtccctc ccttcgaggt ggatattgcc ttgttagcca cggaaaagcc
     7081 cgcgcccgat tctgataggg acgacctagc caatgatgag gatcccgatg atgacaacga
     7141 tggcgtcccc gatgaccaag atgccttccc cctggaacga gaagagtggg ccgatgttga
     7201 cggagaccgc atcggggata attttgatgc cgatatcgat ggggatggtg taggggatga
     7261 tcgcaacggg aacgggattc ccgattatca agagatggac gccgacggcg acggtgttcc
     7321 taaggccaat gccatcccat gggatgcttt tccgcacgat cctcacgagt ggcgcgacac
     7381 cgacggcgat gggataggcg acaatgccga cgacgatgac gacgccgacg gattcaccga
     7441 tcaagaagaa cgcaaggccc gcaccgaccc attgaatcct cttagctttc catagtcctt
     7501 agccgttgcc ccactccaac cagtggagaa aggcagacgc gactcggcat ggctgaatat
     7561 gagcctgcta aaacaggcgg tgtttagttt gccatccatg ttgccacttc cctattgggc
     7621 tgcttttgca atgcaccctc gtgtctttct gtgtctgcga tgagttcaag tgccgggatc
     7681 gtttcagtta cgtctaatga ctaaccggca cgattgccgc gaaggaatct cgaaggcaat
     7741 tcgggtgcgc ggaccggaaa ccgtgaacgc ttcctctttc aaaacatgat cccgggctat
     7801 gatttttagc gtgtagtcgc cgggcttaag catattcagt tctgccccca tcgcccgcgg
     7861 tgatcgacca aagtgaaaca atgtggcctc caaaccgtca ggagctgcca tcctgaccaa
     7921 aaccgcaatc tccctggggc gcgttagcca gcgcaccgca ttaagaggga aagttcccaa
     7981 ccctcccgga tcgcccgtca cggcggaata aagcaatgca gtatccgggg attggaccgg
     8041 aaattttggt tcggccaata ccacgtcgcc ttgaaacacg gcggggagcc tcaatacgcg
     8101 gtcggtgtag cggacttcgc tggtgtaacc gggaaaattg taggaaaagg ccgtggccgt
     8161 gtccgccagg gcggccacaa gtggctgctc atcccccgta agtgcgtagt gaagccaggg
     8221 tgcctgtctc ccttccagca agcgatccca agtcccgtca ccggtgagga agcggtactt
     8281 ggccagtgtc tgccgcaaaa atcccatttg cagggcacac caggcctcgc ttcccggctc
     8341 gatgtccttg ggaaggtttc gcccagaata ccgcagggcc aactccgcca tggagtgaat
     8401 aggacgcagg tatttttcgt ctcctgtcat gtggaatgtg agcagcaaag catcggtgag
     8461 gtaacccatc gcactgggcc actggtaaag cgtcggctca ctgtgattgc gcggatccca
     8521 ccaatgcggt gacaggcccc cgatggaccc gtccggccaa tggatggttg cgggcaagac
     8581 tccagccggc tttccatttt ctgatcgtgc tgcggcatcc acccatgtgt ccgtccactc
     8641 acacaaaact cgcccgaccc gctgatcacc cgtccgcaac cagtacaaaa aaacaggctg
     8701 cactgcgcga acgtgatacg gagtgtcgca tgcccgctga ggatcaggat gaacgcgatc
     8761 taccgtgaaa taggtacttt tgaactgccg aaatccccgc tcattgcggc cggtccagag
     8821 gttttccatc aactccgcta ggcgaaggac ccgctgctgc cacaccaggt tgtccggctc
     8881 caaaagcatc attggcaaga ttgtgtcggt tgagtcctcg gcactatgtt cgacgtcggt
     8941 cattctggac atgtacccgg ccgccatgtg cggttgctga aaaagggctt ccgaaaagcg
     9001 cttctggccg gcacagatct tggggtcatc aaatccaatc aacccgggga cccaaaagcg
     9061 ccacatttcg cagtcatcac cccaaccgcc gccaaaggcc ccatccggct gctgccgatg
     9121 atcgagccac cactcgatga tatcggctag gccttccagt gccacccgct gatggaccgc
     9181 ccactccgga gcctcaggca ctgcttccca atttcgtgtc catggaattc cctcgccgag
     9241 atacatccgc gcaatgcggt tttgtggaaa ggcctggtgg gccctctcga agaactgccg
     9301 cgctatgccg aaccattccc gccttcgatc cccgtagctc caaaggttgc cgttctccat
     9361 cacgacactg agaactgccc ggccacgata aaggtacgtg aggggatata acggcgaagt
     9421 tgccggaatc ccaaagtcaa tgtcgcgatt ttgattcacc tcacccccat agaagtggag
     9481 gttttttcct tctacccact gttcgacaaa gcggatcaat ttctccgcat gttcggccaa
     9541 atcgggatcc acgcccggca aggcccgaat ctgccggaga agctccaatc tttcctcatc
     9601 gtcgtcagtg ttcccggcac gctggaccag ttcctgaagt cgttcttgag caggaacatc
     9661 aatttggaat cctattacca ttcccaagac aaggtttatc ccaagcattc ttaccacctc
     9721 aactttttcg gtttttcgtt ctacggtacg aaaatgtatc aggccagcca ccaaattatt
     9781 caccggcctc caccgagatc accgcttggc aggcccggcg gagatactcc tcatacgccc
     9841 actggccgct gccgcccgtt tgtcgtgtaa tgacaagatc gaggctgggc acaaaggcga
     9901 tcaactgtcc gccggatcca ggcttgaaac gggcgtccgc cgggatccct tcgccgcgtc
     9961 catctgttag tcgtgccggc agttcccacc catgcgagaa ggattcggcg tcccggttaa
    10021 agcatttgat tccgtggatg gagtgcgtgg gcgcggccgt ctcttcgaca aaccatttgg
    10081 gtatgacctg tcgatcaccc caccgtcccc ctcgcaacat gcagtaacag atccgggcgc
    10141 tttcccgcgc cggtagcccc aaggtgtggg agggatgtcg gccaatagtc ccgtccaaat
    10201 acgcgaacca ccagcgttcg atccccaggg gtttgaagag tttctcgatg gcgaattgat
    10261 cataggggac acctgtcacg tcttcgcaca ccagtgcggc atggtaaaag gcatgggtgg
    10321 aataacccag gtctgtccct gggtcaaacg ccagctttgc cgtcacaggg tcaccactgt
    10381 ggccgaggac atagtcccac ggtcctcggt ttttggcccc cgtgtattcg ggtgcgattc
    10441 ccgaggtgtg gttgagcagt tgtcgcacca agatttgctt cttacgggga tcgctcaaag
    10501 gttccgccca aggaatgaac gaaaacgcgg gatcgtcaaa agtcatcttc ttcggtgtcc
    10561 ggccagccaa gctctcttcc gacgcgatgg caagaaccgt ggcacagatc gccttagcac
    10621 acgacttgat attgcgggta tctgtcttgg aactattgcc tcgctcgact tccagtacga
    10681 tataacctcg gcgaatcacc accgcggcga aaggccgttg gtccgacacc agcaaccatt
    10741 ctttgagtac ctccaacttc tgggggtcca tgcccgccag gcgccggata tcggcggggg
    10801 aatccagctt tcgccaccca ccctgcgatt ccagcggcgg gaagtagtcg ctttcggcac
    10861 ccaccgcgga aaaccccacc caacaccata acaaacagcc tgcgaacact aaccgaagca
    10921 tgtgtgcgcc tttctcaaaa gtttcttggc cagagctaac ttgaatcgag ggatgccggg
    10981 ccattttacc cccccgacgg tattatcatg gtcgtttatc aaaacacaag ccactaccac
    11041 tgcatgttga caattttccg tccggtgctg atatgacgtc gtgcctatgt cggacgcaac
    11101 cacccatctc tcggtgggtg tatggtctca atagtctggg ctgccgtatc gcggtaggag
    11161 gtgctttatg aagcgtcgtg gcttgttgtt gggcctggta agttgggttg gtgcactcgt
    11221 ttttgttggc gcgatggggt tgtttggtcc acgggccgat gcccagcagg gtccgccgcc
    11281 ccatgccttg gcccgcgtga tcgctgttca ggaacgccac acggaacgcc tcatgtcgtt
    11341 gcccgatgtg gtgggcacgg ctgtgggcct cgatgaaatg ggtcgcccgg tggtcaaggt
    11401 ctatgtggcc catgcaggcg tgcggggcat cccttccgtc attgatgggg aaaaagtgga
    11461 agtcgaagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aggcgtgcgg
    11581 ggcatccctt ccgtcattga tggggaaaaa gtggaagtcg aagtaacggg cgagatctat
    11641 gccctcgggg cattggcggc cctcgaaaag gctgccaaac cttcagcggt aaaagcggtg
    11701 aatcctcgcg gctggtttga tcggccggtg ccgatcgggg tttccacggg caacgccaac
    11761 gaaggctcag ccggtaccat tgcctgtcgg gttactgatg gaaccaacgt ttatgccctc
    11821 agttgcaacc acgtttacgc ccgaacaaac aacgcgtcac tcgatgaggc gatttggcag
    11881 cccgggccat atgatgtggt gacgagctca ctaataaact ccgaccttga ctttgtcata
    11941 ggcacgttag ctgcttttgt cccaatagat ttctcatcat ccggtaagaa ttatgtcgat
    12001 gcggccatcg cgttggtgag cacggacaca gttggtacga gcacgccgtc tggtggctac
    12061 ggaacaccgt cgagcgagac tagtaccgca tacgtgggca tgcgtgtcca gaaatatggt
    12121 cgaacgaccc ggctcactta tggcacaata agcggagtaa atgcgacggt catagttaac
    12181 tatggaatcg gccaagccaa atttgtcggc cagatcagtg tcaaagggtc tcggtttatc
    12241 caaccaggtg attccggttc gttgctggta actcgaaccg gcaataagcc ggtgggtttg
    12301 ctctttgccg ggaatagctc tgggtctttt gcgattgcca acccaattga cttggtgctc
    12361 gatgagttta atgttagcat cgatggccag taagtccgaa gagacaccgt actaaccaat
    12421 ccaaccgagg ccacccgcgg aggtggtgaa cgactacgca gagcgatggc ttgacctgtc
    12481 tgagatggtc ggcgttgttg tgaggaagac cggggaaaac cgtggagcat ggtcttatat
    12541 agtcgagcgg attaaggaaa ttactaggaa gatcgctcag acgcgatcga aggctgccgg
    12601 gttgttggcg aggggaccgt tagtgccggt tgagcggatt gagatcggcc aaggttattc
    12661 cccgcaggag tgtggtcttc tgaagcgata ggttacgagt ctcgcattgg tggccgaact
    12721 taccgattgg ccgaaagccg cagccaccgc caaaatagcc aaaacagaca aatggcctgg
    12781 gcgttgagga caacgaatag ccggtgctga agtcgttgct ccacaaatag ctgcccgacc
    12841 aatactctcc tcgaacagac cctctctcga ctggttgtcg aaactgacca tacgccaccc
    12901 acttacgtgg ctggcgtccc gtgcgctccc gaggcaacga tggcagccga actatgaacg
    12961 tactcgggtt cggtcgtcct tcaaggaggt gctgtcgtta acccgacagc ctcttttccg
    13021 cgcggcgaga atgccggtcg gttggccaat aaaaatcgca ctcgcgcgaa tcccaccgga
    13081 gcccaaatca ctgagccagc tccccccgtc cacgtcgcag aacctaccgg agcaccctca
    13141 cccccaaccc ctctcccaaa gggagagggg agtttatatg cctcccaggc aagcgccccc
    13201 ggttgacagc cgccgctggt cggtatgccc gtccccgtcg gcgtccagcc gcttgcggac
    13261 ccag
//
LOCUS       DAOSUT010000116        13240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2780_length_13240_cov_9.368146, whole genome shotgun sequence.
ACCESSION   DAOSUT010000116 DAOSUT010000000
VERSION     DAOSUT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_13240_cov_9.368146"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLD05_10980"
     CDS             <1..1191
                     /locus_tag="PLD05_10980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOP78008.1"
                     /translation="HRTDGMMERAFQIVPKEEIFRQTGLQFMQLNTLYQLLAMKEAAS
                     PLLDVAERLLMMPDLFHWMLTGEKCNEFTDATTTQFFNPKERRWATELLERFGLPTKI
                     LGPIVQPGTVLGTLRPSLVAETGLQNAKVVLPGSHDTASAVMAVPARGTSGGVPEWCY
                     ISLGTWALMGVEVAEPVINETSLALNFTNEGGVGNTIRLLKNITGLWLVQECRRVWNQ
                     AGRSWDWEDLNRLTAEAPGLVSFINPDHPMFLAPTDMPEAIREFTRRTHQRVPESEGA
                     VLRCALDSIAMKFRHVLEMCEELIRRRIEVVHVVGGGTKNRLLCQAAADACGRPVLAG
                     PVEATAIGNIMMQAVAAGEVGNIAEAREIVRESFPLDEYTPRDVQAWEDAYSRFLDVI
                     NTEI"
     gene            1302..2324
                     /locus_tag="PLD05_10985"
     CDS             1302..2324
                     /locus_tag="PLD05_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78009.1"
                     /translation="MVRVGIVGIGFMGMIHYLAYQKLKGVKVVAICEKIPERLAGDWR
                     SIKGNFGPKGRKMDLSGIAKYSEIEELLKDPNVDVVDICLPPALHPQATVAALEAGKH
                     VFCEKPIALTTADADRMVKTAQKKGKMLLIGHVLPFFAAYRFAYDAVTSGRYGACLGG
                     QFTRIISDPLWLPDFYNPNVCGGPAIDLHIHDAHFIRLLFGMPKSVHSVGRMRGEVPE
                     FFNTQFLYDDPRLVVTATSGVIMQQGRPFTNGYEIHLEKATLVFDSWMNMPVTILTED
                     GKVKRPKIPTTDPVDDFAAELAEVVRTIKTGEPSKFLDGQLARDALVLAHRQVESVKK
                     RRAVKV"
     gene            2753..4540
                     /locus_tag="PLD05_10990"
     CDS             2753..4540
                     /locus_tag="PLD05_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOP78010.1"
                     /translation="MQQGRDSRLQRIDIRAPRHQRRGGTWGTSVAIKAIVSLFVFCVG
                     SAGWGGLSVFAGDTENAATPVVPAAEQLKFILEQGLSLEHQSRWGEALTLYEDALRQF
                     PDDKVLEQRFHEARLHYDLLRRHSDHTYLELVGRLSSSEVFQIYDEVLLKIHAHYVEA
                     PGWRYLYEQGVHAVAIALTEPKFLAAHRLSISAEATAATARDLPLVVPAQNVQSRMTA
                     IEATTRVANWLRERIGLPPQATVMEMVCGMVNALDPYSAYLTPAQLSELYSQIEGNFV
                     GLGIELRPQEDALVVLRVIPGSPAEKSGISPGEKIIGVDGEGVKDLSPDRAADQLKGP
                     EGSTVTLTVIGKDGQSRQVVVRRTRVEVPSVTDVKIIDPDLKIAYFRLLSFQRTTATD
                     VLAALQRLAQEGMSTLIIDLRGNPGGLLGAAVETADLFLSRGVVVSTRGRNPQENLVY
                     NAHDAGTWQVPLVILIDRESASAAEIFAGAIQDQGRGTVVGVPSYGKGSIQGIFPLNL
                     GNSGLRLTTARFFSPQGRPYAGRGVIPDVRVQTVARPVDASSGGLVWQRPNAGTIDGE
                     TPPLSDDPILEAGLQTARRIVAQRPATTW"
     gene            complement(4568..5572)
                     /locus_tag="PLD05_10995"
     CDS             complement(4568..5572)
                     /locus_tag="PLD05_10995"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78011.1"
                     /translation="MAKKPIDVYRDWLGIEDTNRPLNYYQLLRLPLFHDDINAIREQY
                     RKLNAYVRKFATGDYAFESQQLLNELAKAMLCLTDVQRKREYDAMLGRKDFGEGVRRT
                     VEEVLLANKTITPEALERARRFAQAVGLAVHEAIIQQKLAAPEAVMQAYAESEGLPYL
                     DLEDMTIDESLINVLPPAFVRQNSCVPVMIDDNQVLVASPFPINPDVEEQLRLRFGLP
                     VRTVITTPASLNAAILKYYSGRGGTAASAGPSRGASTPAASAVSKPVTAPRTPERFRQ
                     DLQYTIVAFNLTVILVMLVQFLVRPIYDFGTLLGAFFIAALVGALVGAGTFFYRSQRP
                     "
     gene            complement(5584..7161)
                     /locus_tag="PLD05_11000"
     CDS             complement(5584..7161)
                     /locus_tag="PLD05_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOP78012.1"
                     /translation="MHFEPGHTVGIDLGTTFSAIAILDDEGKPRVIPNEDGDIETASV
                     ILLADSGHVIVGPNRMRAAMEDPQNVIERIKRYMGMPDYRRTFDGREISPEFISALIL
                     KKLRQDAEKQIGKIGNAVITVPYYFNDARRKATQDAGRIAGLNVVDIMNEPTAATLTY
                     AYREGILGNPEVRMDKPKKVLVYDLGGGTFDVTVVEYTPTQFRVLATDGDVQLGGVDW
                     NDRLLDYVADTFKQRHGIDPRESPQMIQILWNDCDLAKIELSEKTETVIHCRYEGRAV
                     DVPITRDLFDSLTADLLQRTADTTLLVLEEAKVRPEELEAIVLVGGSTLMPQVPQMIR
                     RVLNREPYRGISPHTAVAEGAAIHAAILEAKFRGEQSELAERIRRRLQLVQQENVNSH
                     GLGIVALDPKTGKTVNHIMIPRNSRIPIEVRRIFKTTKDNQERITVQVLEGDAPDPFA
                     CSLLGKCRITDLPPGLPKGSPVEVTYAFSAQGRVIVTARDKTSNREARIEIERRGVLR
                     DEEVDALSRLANEYKVD"
     gene            complement(7362..8384)
                     /locus_tag="PLD05_11005"
     CDS             complement(7362..8384)
                     /locus_tag="PLD05_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78013.1"
                     /translation="MTRERTTGLKALGCLVALFVAQILGVTTGCQKSATQGGSKGPDV
                     PRLVSGRLPELGDYLPPLDEGRLEVAPPKEWIVSPRSEKYIVRFQADSGSAYPTVLVM
                     GSNSERFKTVTPENLEAFAKSVAAELESAGTKTTVKMGRIGDLIGVTYTRRAKVKDNF
                     GGIVERLFFDTVVAGRRYQFELRCPPEMVGLAEPYFYAVVSGAKVHAVGELESEEELE
                     SGEVTETPPKAEEQPAGQAQQKPAEKEPPVAEQAKVEAPKPEAKPAQKPTSQKTLPPE
                     KEKGQAKPAEQAPAEKPKEEAKKPEEPPAESQSAKEASQESKPEEKPKGKETGDLLKD
                     LDALLQ"
     gene            complement(8541..8855)
                     /locus_tag="PLD05_11010"
     CDS             complement(8541..8855)
                     /locus_tag="PLD05_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78014.1"
                     /translation="MARRTLDRKALRQQTEAAEALQGETSSEKAPARTRRSKPKVKKQ
                     TKLVALWGVFNQALVEVQTFDYSRRLDAEKLAKELSDSKKVPHFVQLVKRQVEVEIPS
                     EE"
     gene            9143..>9360
                     /locus_tag="PLD05_11015"
     CDS             9143..>9360
                     /locus_tag="PLD05_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HOP78015.1"
                     /translation="MSEADSDHTRSPAALKVRSFPHKPGVYLMKDEAGRVIYVGKAKD
                     LRHRAGSYFLKAAAEDPRTARLVREIRD"
     assembly_gap    9361..9460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9461..10348
                     /locus_tag="PLD05_11020"
     CDS             <9461..10348
                     /locus_tag="PLD05_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOP78016.1"
                     /translation="QVLQKIFRFRTCSLDIEENDPKWKWFRPCLLASINQCSAPCNLR
                     ISKEEYRKDIRRLQRFLEGDKEQLLVDLRREMKEAAQALRFEEAARLRDEIEYLEKLD
                     ERGKIDVHVQPEAFHIDPQKGLAGLQRILKLPTLPRAIEGVDIAHLAGSETVASLVRF
                     IDGLPFKPGYKRYRIRTVEGVDDYAAIREVVYRRFRRLREEDESPPDILLIDGGKGQL
                     HAALSALEEWDGARPIILSLAKREEELYLPDRDEPLRLSRHSFALRLLQYVRDEAHRF
                     AQHYHHLLRRKSTLGDEEA"
     gene            complement(10378..11322)
                     /locus_tag="PLD05_11025"
     CDS             complement(10378..11322)
                     /locus_tag="PLD05_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOP78017.1"
                     /translation="MDQTQFLKRKLLECLGGPWPVPCDLNPEWGDPEDKGDYTLTRVT
                     YEVEPGDRVPALLLVPKTADREHPAPGILVWHQHNGEWHLGKSEPAGLAGNPMHHTGV
                     SLVREGYVVLCPDALCFEERQSPHLKGGDYERFVFLRYVVQGKCLAWKNILDMRRAVD
                     LISSLPFVDANRLGCYGHSMGSTFTWLVGPWEERLCCLVGNCCLPTYAAIHRTHILHC
                     FPNFIPGWMKYGDTPHIAGLIAPRALHLNFGELDGGSPLPEVREGIEIIRGFYRQAGA
                     EDNFRAFIQPGVGHVLSPEMWEQTREWFAIHLKGKRSA"
     gene            complement(11418..12752)
                     /locus_tag="PLD05_11030"
     CDS             complement(11418..12752)
                     /locus_tag="PLD05_11030"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOP78018.1"
                     /translation="MSFAQRKQLGLVKHQPWLLGFAVTTALFLLTGGCREPSVADRPS
                     PSAARKAPATKFDGEAGAKTATKSEGLTTSAETYDHLDIAIDAPTEWTVGDEVSIQLT
                     LSNHSDHTIEGTELRAEIQFGLQDSISEGHIVVPIASLPGKTSYHSMANFVSSRPGIW
                     YVKAAVYLQGELIKATTRTLYIREATGREEPKPADDTDESLGPPLVDHPERLQRLHPD
                     FPIWIDAEGHALVMIGRVCQREVPLELFACLKNSKEHESIVSINTRAYIVHAGLLALG
                     VEPGRPVQFYPQYRPAEGPEIDIVVAWKDAAGNIHRMPAQQWVRHAQTKEPLELSWIF
                     TGSQIVKDEDTGDQYYYADSTGELICVSNFPSAVLDLPIRSTDSNESLLFEAFTENIP
                     PLGTPVTLILSPKSSTTSPTSGKPSAENVSPSTESGETAPDSVQPSGDEPAG"
     gene            13078..>13240
                     /locus_tag="PLD05_11035"
     CDS             13078..>13240
                     /locus_tag="PLD05_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP78019.1"
                     /translation="MNRIKGSRGFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQ
                     CTNNLKQIGL"
BASE COUNT         2745 a         3554 c         3749 g         3092 t
ORIGIN      
        1 caccgcaccg acggcatgat ggagcgggcc tttcagatcg ttcccaaaga ggagattttc
       61 cggcagactg gtctgcagtt catgcaattg aatacgctat atcagctttt ggccatgaag
      121 gaagccgctt cgcccttgtt ggatgttgcg gaacggcttc tcatgatgcc ggacctcttt
      181 cactggatgt taactggcga gaaatgtaac gagtttaccg acgccacaac gactcagttt
      241 ttcaatccca aggagcggcg ttgggctacc gaactgttgg aacgtttcgg cttgccgacc
      301 aagattctcg gcccgattgt ccagccgggg acggtccttg ggacactgcg tccgagtttg
      361 gtggccgaga caggattgca gaacgccaag gttgtcttgc cgggttcgca cgacacggcc
      421 agtgcggtta tggccgttcc cgcgcggggg accagcggtg gtgttcccga gtggtgttat
      481 atcagcctag ggacctgggc gttgatgggc gtggaagttg ccgagccggt catcaacgag
      541 acatccttgg ctctcaactt cacaaatgaa ggcggtgtgg ggaataccat tcgactcctc
      601 aaaaacatta cagggttgtg gttagtgcag gaatgccgtc gtgtctggaa ccaggcaggt
      661 cgcagttggg attgggaaga tttgaatcgt ctgacggcgg aggccccagg tctggtgagt
      721 ttcatcaatc cggatcaccc gatgttcctt gctcccaccg acatgccaga agccattcgg
      781 gaatttaccc ggcgcacgca tcagcgtgtg cccgaaagtg agggggctgt cctgcgatgc
      841 gccctcgaca gtatcgccat gaagttccgg cacgttttgg aaatgtgtga ggaattgatc
      901 cggcggcgaa tcgaggtcgt acacgttgtt ggtggtggta ccaagaatcg gttactctgt
      961 caggctgctg cggacgcgtg tggccgaccc gtcttggcag ggccggtgga ggccacggcc
     1021 atcggcaaca tcatgatgca ggccgtcgcc gcaggtgaag ttggaaatat cgccgaggca
     1081 agagagatcg tccgcgagag tttcccgctg gacgaataca ctccgcgtga tgtccaggct
     1141 tgggaggatg cctattctcg ttttctggat gtgatcaaca cagagattta acgatttacc
     1201 aatgacgggt tcgtgggaag tctctagggt tcgttagccg aacgaggaga cgccgttttg
     1261 gggagttgct tgtgaggctg tcagcatagg agaagtgaga tatggttcgc gtgggaatcg
     1321 tcggcatcgg gttcatggga atgatccact atttggccta ccaaaagctc aagggcgtca
     1381 aggtagtggc catttgcgag aagataccag aacggctggc cggggactgg cgatccatca
     1441 agggcaattt cggccccaag gggcggaaaa tggacctttc cgggattgcc aaatactctg
     1501 aaattgagga acttctcaag gacccgaatg tcgatgtggt cgatatatgt ctccctccgg
     1561 ccttgcaccc acaagcaact gtggccgctt tagaggcggg caaacacgtg ttctgtgaaa
     1621 aacccatcgc actgacgacg gccgatgccg accgaatggt gaagacggcc cagaaaaagg
     1681 ggaaaatgct ccttattggc cacgttttgc cattcttcgc ggcctatcga tttgcctatg
     1741 atgctgtgac gagtgggcgc tacggggcgt gccttggcgg tcagttcacg cggatcattt
     1801 ccgatcctct ttggttgccc gatttctata atcccaacgt ctgcggcggt ccggcgatcg
     1861 atttgcacat ccatgatgcc catttcattc gcttgctgtt tggcatgccg aagtccgtcc
     1921 acagtgtggg gcgaatgcgg ggtgaagtgc cggagttctt taacacccag ttcctctatg
     1981 atgacccacg gttagtggta acggccacca gtggagtcat tatgcaacag ggaaggccgt
     2041 tcaccaatgg gtacgaaatc catctcgaaa aggcgacact tgtcttcgat tcctggatga
     2101 atatgccggt caccatcctc acggaggatg ggaaggttaa acgccccaag ataccgacaa
     2161 ctgatccggt tgatgatttt gccgcagagt tggcggaagt ggtacggacg atcaagaccg
     2221 gagaaccgtc aaagtttctc gatgggcaat tggcgcggga cgctctggtc ctggcccatc
     2281 gacaggtcga gtccgtgaaa aagcggcgag ctgttaaagt ctgaagccgt ttaagcgtga
     2341 tctcgcttga aacatattga aggtttcact cttaaagtgc gcatcccgct ctcctgcggg
     2401 atgtttttgt aaatggcagt ctcgccacaa ttggtcattg cggttttcgg ggcattcttt
     2461 ctgtggccct caattccggc aggccaacag tcggttattc caatcgtatc gataaaacat
     2521 tgacaaaact cggttagtgc ttttgcatgg tgccgattcg taagatatac tcattcgaac
     2581 ccgttaatcg gatgacgaat cccatcgggt ttcgaatggt gggggcgttt ttctccgcat
     2641 aagcggaaca gttgcactgg acgaaatagg tcaacaagtc gctatttcta gccatctccc
     2701 caattgatta ggtcacaaga cgtccgtccc ttaaccctgg attaggcccg ctatgcaaca
     2761 aggacgcgac agtcggctgc aaagaatcga catccgcgca cctcgtcacc agcgacgtgg
     2821 cggtacttgg ggcacctcag ttgcaataaa ggcaatcgtt tccctttttg ttttttgtgt
     2881 gggttctgcg ggctggggcg ggcttagtgt ttttgccggg gataccgaaa acgccgctac
     2941 tccggttgtt cctgcagcgg agcagctaaa gttcatcctg gaacagggct tgagcctcga
     3001 gcaccaatca cgttgggggg aggccctgac tctttacgag gatgccctcc ggcaattccc
     3061 ggatgacaag gtcttggagc aacggtttca tgaagcccgg ctgcattacg atcttttgcg
     3121 gcgtcacagc gaccatacgt atttggaact ggtaggtcgt ctcagttctt ctgaagtgtt
     3181 tcagatctac gacgaggtcc tgctcaaaat ccacgcccat tatgtcgaag caccgggatg
     3241 gcgctatcta tacgaacaag gcgtccacgc ggtcgcgatt gcattgacgg agccgaaatt
     3301 tttggccgcc catcggcttt ccatttctgc ggaggcgact gcggcaacgg cccgagattt
     3361 acctctggtt gttcctgccc aaaatgtcca atcaaggatg acggccatcg aggccacgac
     3421 tcgggtggcg aattggttgc gcgaacgaat cggcttgcca ccgcaggcca ccgttatgga
     3481 aatggtttgc ggcatggtta acgcgctcga tccctattcc gcgtacctta caccggcgca
     3541 gctttcggag ctctattctc agatcgaagg taattttgtc ggtctgggaa tcgaattgcg
     3601 cccgcaagag gatgccctgg tcgtgttgcg ggtgattcca ggaagtccgg cggagaaaag
     3661 cggtatctct ccgggcgaaa aaattatcgg tgtcgatggg gagggtgtta aggatttgtc
     3721 acccgatagg gcggctgacc aattgaaggg accggagggt tcaacggtca cactaacggt
     3781 aattgggaaa gatgggcaat ctcgtcaggt cgtggtccgc aggacgcgcg ttgaggttcc
     3841 aagtgttact gatgtcaaaa tcattgaccc ggatctcaag atcgcttatt tccgcttgtt
     3901 gagttttcag cggaccacag ccaccgatgt cctggcagca ttgcaaaggc ttgcccaaga
     3961 ggggatgtca acgctgatca tcgacctgcg tggtaatccc ggtggacttc tgggagcggc
     4021 cgtagaaacg gcagaccttt ttctttcgcg tggtgtcgtt gtgagcaccc gcggtcgtaa
     4081 tccccaggag aatctggtct ataacgcaca cgatgcgggt acctggcagg tccccctggt
     4141 cattttgatt gaccgggaga gtgccagtgc cgcggagatt ttcgccggag ccattcagga
     4201 tcaagggcgg ggtacggtcg tgggtgtgcc gagttatggc aaaggctcaa tccaggggat
     4261 tttccctcta aatctcggca attcggggct gcggctgacg acggcccgat tcttctctcc
     4321 tcaaggccgg ccctatgccg gccggggggt gatccccgat gtccgggttc aaaccgtggc
     4381 gcggcctgtt gatgcgtctt ctggagggct tgtctggcag cggccaaacg caggcacgat
     4441 tgatggcgag acgccacctc tttctgatga tcccatcttg gaagcgggac ttcaaacagc
     4501 ccggagaatc gtcgcccagc gcccggcaac tacatggtaa tcgttgactg ctcgcgccgg
     4561 ctctttctca tggccgttga gaacggtaaa agaatgtgcc cgcaccgacc agagcaccca
     4621 cgagcgcggc aatgaaaaat gcccctaaga gtgttccaaa gtcatatatt ggccgcacta
     4681 aaaattgaac aagcatgacc aggatcaccg tcagattgaa cgccacaatt gtgtactgga
     4741 gatcctggcg gaatcgctca ggtgtccgcg gcgcggtcac aggctttgaa acggcacttg
     4801 ccgccggagt ggaagcaccg cgactcgggc cagctgatgc cgccgtgcca cctcgacctg
     4861 aatagtattt caagatggcg gcattgagac tggcaggagt ggtgatcacg gttcggaccg
     4921 ggaggccaaa gcgaagacgc aattgttctt cgacatcagg gttgatcgga aagggggatg
     4981 cgacgagcac ttggttgtcg tcgatcatca caggcacgca tgagttctgc cgaacgaacg
     5041 ccggaggtaa aacgttaatg agcgattcgt caatggtcat gtcctccaag tcgaggtaag
     5101 gcaagccttc cgactcggcg taggcctgca tcacggcctc cggcgcggcc aacttttgtt
     5161 gaatgatcgc ctcgtggacg gccaacccaa cggcctgagc aaaacgcctt gcccgctcca
     5221 atgcctccgg cgtgatcgtc ttgttggcca gaaggacctc ctctacagtg cggcggacgc
     5281 cttccccgaa atcttttcgg cccagcatcg cgtcatattc acgctttcgc tggacgtcgg
     5341 tgaggcacaa catggccttg gccaactcgt tgaggagctg ctgtgactcg aacgcgtaat
     5401 cgcctgtggc gaactttcgc acgtatgcgt tcagtttgcg atactgttcc cggatggcgt
     5461 tgatgtcatc atggaacagg ggcagacgca aaagctgata gtaattgaga ggtcggttgg
     5521 tatcctcaat tcccaaccaa tcccgataaa catcaattgg cttcttggcc atggttgccg
     5581 gtcctaatca accttgtact cgttggccag tcgcgacagt gcatccactt cctcgtcgcg
     5641 gagaacgcca cgacgttcaa tctcgatacg tgcctcgcgg ttactcgttt tatcacgggc
     5701 tgtcacaatc acgcggccct gggcgctgaa cgcatacgtc acttctaccg gcgatccctt
     5761 ggggagacct ggcggcaaat cggtgatgcg acacttaccc aacaaggaac aggcgaaagg
     5821 atcgggcgca tcgccttcca gaacctggac agtaatccgt tcctggttgt ctttggtcgt
     5881 cttgaagata cgacggacct cgattggaat ccgactattt cgcggaatca taatgtgatt
     5941 aaccgttttg cccgttttgg gatcgagggc cacgatgccg aggccgtggg agttaacgtt
     6001 ctcttgctgg acgagctgca agcgacggcg aattcgctca gccagttcgc tttgctcgcc
     6061 tcggaatttt gcttcgagga tagccgcgtg aatggcggcg ccttccgcca cggcggtatg
     6121 aggcgaaatg ccgcgatagg gctcccgatt gagaacccgc cgaatcatct gcgggacctg
     6181 cggcatcaag gttgatcctc ccacgaggac gatcgcctca agttcttccg gacggacctt
     6241 ggcttcctcc agcaccaaga gcgttgtgtc ggcggtccgc tggagcaaat ccgccgtgag
     6301 cgaatcgaac aagtcgcggg taatcggcac atcgacggcc cgcccctcat aacggcagtg
     6361 gatgacggtt tctgtctttt cggagagttc gatttttgcc agatcgcagt cgttccacag
     6421 gatttgaatc atttgcggtg attcgcgcgg gtcaatcccg tgccgctgct tgaatgtgtc
     6481 ggccacataa tcgagcaggc ggtcgttcca gtccacgccg ccaagctgca cgtcaccgtc
     6541 ggtcgccagc acacggaatt gggtgggcgt gtattccacc acagtcacgt cgaaggttcc
     6601 accccccagg tcatacacga ggaccttttt gggtttgtcc atgcggacct cggggtttcc
     6661 aaggataccc tcgcgatagg catacgtcag tgtggccgcc gtcggctcgt tcatgatgtc
     6721 aacgacgttt aagccggcga tgcggcccgc atcctgagtg gccttccgtc tggcgtcgtt
     6781 gaaatagtaa ggaaccgtga tcacggcatt gccgatcttg ccgatctgct tttccgcgtc
     6841 ttggcgaagc ttcttaagga tgagggccga aatgaactct ggcgaaattt cacggccatc
     6901 aaacgtcctt cgatagtcgg gcatccccat gtagcgcttg atgcgctcaa tgacgttttg
     6961 ggggtcttcc atagctgccc gcatccgatt gggaccaacg atcacgtgtc cgctatcggc
     7021 gagaagaatc acactcgctg tttcgatgtc tccatcttcg ttgggaatga cccgcggttt
     7081 tccttcatca tcaagaattg cgatcgcgga aaacgtggtc ccaagatcga ttccaacggt
     7141 gtgaccaggt tcgaaatgca tcgttcgcct tcccgacaaa aagatgtgct caaagaatac
     7201 ctcaagacga ccgattgtcc tttcgcccaa taaccggatc cctggcaaac ggcagtttct
     7261 gacaaagcag acttgctttt cattgtgatc agaggcggac gcgatgtcga tatccgggca
     7321 aaattttgtc ggccgtgact gatttggcgc cctcccaggg attactgcag gagtgcatcg
     7381 agatccttga gtaagtctcc cgtttccttc cctttgggtt tttcctcggg tttactttct
     7441 tgagaggcct cctttgccga ttgggactcc gccggaggct cttccggttt tttagcttct
     7501 tccttgggtt tttctgctgg cgcctgctcc gccggcttgg cctggccctt ttctttttct
     7561 ggcggaagcg tcttttggct ggtcggcttt tgggccggct tggcctcggg cttgggagct
     7621 tcaacctttg cctgttccgc aaccggcggt tccttctcgg cgggcttttg ctgagcctgg
     7681 ccggcaggtt gttcttctgc ctttggcgga gtctcggtca cttccccgct ctcgagctct
     7741 tcttcgctct ccaattctcc aacggcgtgg accttcgcac cgctgacgac ggcgtaaaag
     7801 tacggctcag caagcccgac catttcggga gggcatctaa gctcaaactg gtatctccga
     7861 cccgccacga ccgtatcgaa aaagagccgt tccacgattc cgccgaaatt atccttgacc
     7921 ttcgcccggc gggtgtaggt aacgccgatg aggtctccaa tccggcccat tttgaccgtc
     7981 gtcttggtgc cagccgattc caattcggca gcaacagact tggcaaacgc ctccaggttc
     8041 tcgggcgtaa ctgtcttgaa tcgctcactg ttgcttccca tgaccaacac ggtgggataa
     8101 gcactaccgc tatcggcttg gaaacggacg atgtattttt ccgaacgcgg cgagacgatc
     8161 cattcctttg gcggggctac ctccagccgt ccctcatcga gtggtggcag atagtccccc
     8221 agttccggca aacgcccaga gacaagacgc ggcacgtcgg ggcctttcga gccgccttgc
     8281 gtcgcagact tctggcatcc ggtggtgaca cctaggatct gagccacgaa taacgcaacc
     8341 aagcagccga gcgctttcaa gccggttgtt ctctctcgag tcatcgcaac ggttccgtca
     8401 atcctacctt ccgccacccc ttagcttttc catctttatt aacgctttcc aacggtgaga
     8461 aaatgcagaa ggacactaac ccccggctcg ccgatagcgg gcaacactcg ccgcatgatt
     8521 ccagccaggc cagcagtcca tcattcttcg ctggggattt ccacctcgac ctggcgcttg
     8581 acgagttgaa caaaatgcgg cactttcttg gagtctgaaa gttccttggc cagtttttcg
     8641 gcatcaagtc gccgcgagta gtcgaaggtc tggacttcca ccaacgcctg attgaagaca
     8701 ccccacaggg ccaccaactt ggtctgtttc ttgacttttg gtttggagcg tcgagtcctg
     8761 gcaggtgcct tttccgagct tgtctcacct tgcaaggctt ccgcagcctc tgtctgctgc
     8821 cgcaaggcct tccggtcaag tgttctccga gccattgcca tcacctaaga aaaatggggg
     8881 ccaagacctg ccgggaaggg gacccgccac gcggtttgcc cttcgccatt accagttcca
     8941 attctctatc ataattgttg acatggtttt tcgctagcgc atacagatta ggtggtaact
     9001 tataccacga ggatgagggg gtagcgcctt ccagtatgac tgatgctatg gcacccggat
     9061 gtgggcgtca ctctccccga aaaacccttc ttttgcggca agtcgtataa tcatgtgaag
     9121 gatggtcttg cggagtttcg gtatgtcgga agcggattcg gatcatacgc gttcgccggc
     9181 tgctcttaag gttcggagtt ttccccacaa gcctggggtc tatttgatga aggacgaggc
     9241 cggccgcgtt atttacgtcg gcaaggccaa ggatttgcgt caccgggcgg gtagctactt
     9301 cctgaaagca gcggcagagg accctcgaac tgcccggctt gtccgcgaga ttcgcgatat
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caggtcctgc aaaagatttt
     9481 ccgatttcgg acctgctcac tcgatatcga agagaacgac cccaagtgga agtggtttcg
     9541 tccctgtctt ttggcttcga tcaatcaatg ctcggccccg tgcaacctgc ggatttccaa
     9601 ggaggaatac cgtaaggaca tccgtcgcct ccagcgattt ttggaaggtg ataaggaaca
     9661 gctcctcgtc gatctgcgcc gggaaatgaa ggaggcggcg caggcgctgc gatttgaaga
     9721 ggcagcccgt ctgcgggacg aaatcgaata ccttgagaag ttggacgagc gtggcaagat
     9781 tgatgtccac gttcagccgg aggcgtttca tattgatccc caaaaaggat tagccgggct
     9841 gcaaaggatt cttaagctcc ccacgttacc tcgggccatc gagggcgtcg atattgctca
     9901 cctggcaggt agtgaaacgg tggccagtct ggttcggttt atcgatggct tgccgttcaa
     9961 gccaggctat aaacgctatc ggatccgcac ggtggaaggc gttgatgact atgcggccat
    10021 ccgagaagtc gtctatcgcc ggtttcgccg actccgcgaa gaggacgaat cgccgcccga
    10081 tattttgctt atcgacggcg gcaagggtca acttcacgcg gccctttcgg ccctggaaga
    10141 gtgggatggc gcccggccta tcattctttc cttggccaag cgggaggagg agctttatct
    10201 tcccgatcgg gatgaacccc tccggttgag ccgtcattcg ttcgcgctgc ggctgctcca
    10261 gtatgtgcgg gatgaagccc atcgatttgc ccaacactat catcacttat tgcgccgcaa
    10321 atcgacgttg ggtgatgagg aagcataatt gcgagacttc ttgaagggcc tccgcagtca
    10381 ggcggaacgc ttacccttga ggtgaatggc aaaccactcg cgggtttgct cccacatttc
    10441 cggcgaaagg acgtgcccca caccgggctg aataaaggca cgaaagttat cttccgcacc
    10501 ggcttggcga taaaaaccgc ggatgatttc gatgccttca cggacctcgg gcagcgggct
    10561 gccgccatcg agctcaccaa aattgaggtg aagtgcccgc ggggcaatca ggcccgcaat
    10621 gtgcggcgtg tcgccatatt tcatccatcc tgggatgaaa ttaggaaagc aatgaagaat
    10681 atgtgtccga tggatggcag cataggtagg caggcaacaa ttgcccacca agcaacagag
    10741 tcgctcttcc cacgggccga caagccaggt aaatgtcgag cccatggaat gtccgtaaca
    10801 acccagccga ttggcatcga caaacggcaa cgagctgatc agatccactg cccgccgcat
    10861 atccaaaata ttcttccaag ccaggcattt tccttgcacc acataccgca gaaagacaaa
    10921 ccgttcgtag tcgccccctt tgaggtgtgg gctttgcctt tcttcaaagc acagggcatc
    10981 ggggcagagc accacgtagc cctctcggac aagggatacc cccgtatgat gcatgggatt
    11041 gcccgccaat cccgccggtt cgctctttcc caaatgccat tccccgttat gttgatgcca
    11101 aacgagaatc cccggtgccg gatgctctct atccgcagtt ttgggcacca aaaggagagc
    11161 cggaacacga tctcctggtt cgacctcata cgtgacgcgg gtcagtgtgt agtcgccttt
    11221 gtcctctggg tcgccccact cgggattgag atcgcagggt acaggccacg gcccaccaag
    11281 acattcgagc aattttctct tcaggaattg cgtctgatcc attgctcgat ttcctatagc
    11341 ttcgtctcat tggcagttgc aacaaagcgc actcgtttat tgcatataaa cgtttgctct
    11401 gcaagctaag cggcctctca tcccgcgggt tcgtcaccgg aaggctgcac ggaatctgga
    11461 gccgtttcgc cggattcggt ggaagggctg acgttttcgg ctgatggctt ccctgacgtg
    11521 ggcgatgtcg tagatgactt tggagagaga atcagtgtca ccggtgtgcc cagtgggggg
    11581 atattctcgg taaaggcctc gaagaggagg gactcattac tatcggtact tcggatgggc
    11641 aggtccagaa cggccgaggg aaaattggaa acacaaatca attcaccggt gctatcagcg
    11701 taataatact gatctcctgt gtcttcgtcc ttgacgattt gactacccgt gaagatccag
    11761 gaaagctcaa gaggctcttt agtttgggca tggcggaccc actgttgggc gggcatgcga
    11821 tggatgttac cggccgcatc tttccacgcc acaacgatgt cgatttcagg cccttccgcg
    11881 gggcgatact ggggatagaa ctgcaccggg cgtcccggtt cgaccccgag ggccaaaaga
    11941 cctgcatgaa caatataggc tcgagtgttg atcgagacga tggactcgtg ttccttggag
    12001 ttcttaaggc aggcaaatag ctccaagggg acttctcgct ggcagacccg accgatcatg
    12061 acgagggcat ggccttcagc atcgatccag atggggaaat ccgggtgaag ccgttgcaat
    12121 cgctccgggt gatcgaccag cggaggcccc agcgactcgt cggtatcatc agccggtttt
    12181 ggctcctctc gaccggtggc ctcgcggatg taaagggttc gcgttgtggc cttgatcaac
    12241 tcaccctgga gatacacggc ggccttaacg taccaaatcc ccggacggga tgaaacgaaa
    12301 ttggccatcg aatggtacga cgtcttgccg ggcaaggagg caatgggcac cacgatgtgg
    12361 ccttcgctga tactatcctg aaggccgaac tgaatctccg cgcgtagctc cgtgccctca
    12421 atggtatgat cactgtggtt gctcagggtg agttggattg agacctcgtc tccgacggtc
    12481 cattccgtgg gggcatcaat ggcgatatcc agatgatcgt aagtctccgc ggaggtggta
    12541 agcccttcgg acttggtggc cgtcttggcc ccggcctcgc catcaaactt ggttgccgga
    12601 gcttttcgtg cggcactggg tgatggccta tctgccaccg agggttcccg acatccgccg
    12661 gttaacagga aaagcgctgt tgttaccgca aatcccaata gccacggctg atgtttcaca
    12721 aggccgagtt gcttccgttg tgcgaacgac atcggttctc ctcgatacga agtcttccca
    12781 aactgaccct gtggaaccta tggggacgta cagtcgtttt tcaagcagca aacccccgtt
    12841 ggggcatcct atcatgattc tgtgaggaca accagatttg aatctatcaa aaaagaggga
    12901 taccgttaat tgtcaaataa tactgtgtga tttgtacatg aacgtcgctt cggctcgttg
    12961 ctggcgctta ggattttgtg aaagcaaaaa gattgcaccc ggttgtttgg gtcgttataa
    13021 tcgcctttgg ttgtctgggg ttactagggt tcgtggtgat gtgtaggagg ttttcttatg
    13081 aaccggatta agggtagtcg ggggtttacc ctggtcgaat tgctggtggt catagccatc
    13141 atcggcatct tgattgcgtt gctcttgccc gcggtccaag ctgcccggga agcggcccgt
    13201 cgtagtcagt gtacgaacaa cctgaagcag atcggtttgg
//
LOCUS       DAOSUT010000117        13222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2787_length_13222_cov_7.611833, whole genome shotgun sequence.
ACCESSION   DAOSUT010000117 DAOSUT010000000
VERSION     DAOSUT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2787_length_13222_cov_7.611833"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLD05_11040"
     CDS             <1..867
                     /locus_tag="PLD05_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78020.1"
                     /translation="GADFLEPFRPDWAEVSAETWAVVEPELPGTWQGVEEVIASLSEK
                     HRRFLLKQPMTPVGLGLTQSILRCVEARERWPEVPLVMDLCPLLYHCPADTGPMLLVL
                     LGLAEEMGARWITTTHEVNTARTSVRECHVIRRLTHCAKQFHVTLRNVDSSFAMLRDH
                     QISDGDEKYLEELRARLRDPSPRLFVVKDQLRSWAFGQELQAEDAYQLFDKILAAAES
                     QGREIDVDTAFYLGYELCKASIARQLGKNYCQDEALHWGLHTIPEPSPRERRIQRLKR
                     RRGRAGCSEGKN"
     gene            878..2332
                     /gene="pabB"
                     /locus_tag="PLD05_11045"
     CDS             878..2332
                     /gene="pabB"
                     /locus_tag="PLD05_11045"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOP78021.1"
                     /translation="MSRSLPLVVEVPYADWRRSVAVFAQLPWLVVFDSAQEDPRLGRF
                     SFVAADPFERWLVQADASGQAAREAFQSLAERTRCFRQVSCPDLPPFQGGAAGLWGYE
                     LNRAFEDVPAARIDDMRTPLLAVGLYDIVLAVDHERGRAWLISQGFPETDPASRARRA
                     RERLDFFRDRLREPAEANGRPIASTMAPREALPGQVAVFQSLTGEGPHFWSNFSEEAY
                     LAAVSQVVEYIRAGDVFQVNLSQRLACPAAIDSLSLYELLREENPAPFAGYCDLGQWQ
                     ILSSSPERFLRVRDRWVETRPIKGTRRRTHDPDTDRRIARELLHSQKDRAENVMITDL
                     LRNDLSRVCLAESVCVSSLCHLETYEAVFHLVSVIEGRLRESYDAFDLLAASFPGGSI
                     TGAPKIRAMEIIAELEPHARGAYCGCLGYVGFNGDADFNILIRTLTAAKGWWVFSVGG
                     GILATSRPEDEYAETWHKAEGLLRAIRAAYQRGG"
     gene            complement(2352..3332)
                     /locus_tag="PLD05_11050"
     CDS             complement(2352..3332)
                     /locus_tag="PLD05_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78022.1"
                     /translation="MTTIIRKWLASLSLSSMVFILLLVGCHRPPAQQSQPTREEKPSQ
                     TAATTAESGDQATPEATATPRASRLRDVVPAELTAAMQKANPTFRGEVIVENQDGAVV
                     VAINDPEVHDISPLAGLRIHKLDLYQCSVSDLSPLRGLPLTAMALDRTQVKDLRPLAG
                     MPLEYLSLSETEVSDLSPLAGCPLKQLNLVRTKVTDLSPLAGCPLETLWLNDTPVSDL
                     SPLRSVPLVSLTIAGTKVRDLSPLKGMPLKRLHIARSEVTDLTPLRWLRLERLIFTPS
                     KIESGMDVVRNMPTLREIGTAFGESDWGIADRVMPPAEFWELYDAGQLKE"
     gene            3839..4264
                     /locus_tag="PLD05_11055"
     CDS             3839..4264
                     /locus_tag="PLD05_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOP78023.1"
                     /translation="MISRTAIHGLRALMILAEMPPDQYAGTAELAKQIKAPRNYLGKL
                     LQRLADEGLLESQRGKGGGFRLARPPERITIYDVVEPLDRVSRWEGCLLGRSRCSDEA
                     PCPVHVRWAKVRAVYLEFLRTTTLDELVAQRRESPSCRS"
     gene            4515..5114
                     /locus_tag="PLD05_11060"
     CDS             4515..5114
                     /locus_tag="PLD05_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOP78024.1"
                     /translation="MKPTDILRAEHRVIEQVLAVLEKMATQAEASKSVDVESARQVID
                     FIRHFADRCHHAKEEDRLFPALEARGFPRQGGPTGVMLAEHEMGRECVRDMDRALGEL
                     DHAPEAALSTFVTAARRYVALLREHIGKEDNVLFVLAERTIEEDAQAQLLSEFEAVEK
                     EHVGEHVHEKYVQLAESLMERYGIDKQSIPRSGDFFCCH"
     gene            5387..7678
                     /locus_tag="PLD05_11065"
     CDS             5387..7678
                     /locus_tag="PLD05_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOP78025.1"
                     /translation="MGMRSLRNGAVVSFLVGMALLLVGGFFAVDHVPPIPKKIEWQGQ
                     VLADHEAIMRGQDVYQRYGLMDHGSVWGHGSLRGMDFSAYTLHLLGYLVRQWHLGNGR
                     SDENLWEQLPPAPRRELDQTDMLVIQELKHNRYNAETGTLELTEAQAYAFRQLEKFWD
                     KTFGQGDSHYGFLPNTIPSEQERRDIAAFFFWTAWAAVTPRPGLDYSYTNNWPPDITV
                     GNRASSEAVLYSILSVLALFVVLGLVVYIVHRYEFFYGESRAVEAAYRLMKTPVTPSQ
                     RASAKFFLVAGLLFIVQILNGGLLAHYTVHPGKFYVEIVGQMYPYSWAKTWHLQLAVL
                     WIAISWMGTAIYLAPLVGGREPKGQRLLVNILFVAAVAVTVASLIGEVLGIKGYLTHL
                     GPSAWFWLGHQGWEYLELGRLWQILLFGGLIYWLVIVYRAIAPGLRRNQNQADTEEDA
                     HRRALILFYLFSGTFVVLFFGFGLLYGRGTHLTIADYWRWFVVHIWVESIFEFFGVAV
                     ISLFLVTLGLVTAKSALRVAYLTAILVFLSGIPGTAHHYFWYGGPSFWLAIGGVFSSL
                     EPIPLILLVVRAWMEYKSIREAGHEFPYRWPLYFLTASSFWNFLGAGVFGFMINLPLI
                     NYYEHATYLTSNHGHTALFGVYGMLAIAVILFSWRGLVENSAWNDRILKISFWGLNAG
                     LFLMFLTGLLPIGLLQVYHAYANGFWWGRSAAFYEMPLIQALGNFRIIPDTIIIVVGA
                     LPLLYFLLRTFPRLRTAQGSEQG"
     gene            8217..9362
                     /locus_tag="PLD05_11070"
     CDS             8217..9362
                     /locus_tag="PLD05_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOP78026.1"
                     /translation="MQLWHSLSQLRWILLSCLGALSVTVLLEPSETCPGSAMARAEAT
                     PRAEPAVELSTDYRDEGPGYICEIRNGRTFYGDTSRRGVPFAKDPSVVRFGDRYLMYY
                     SIPPFAKDRPNNGWAVGIAESRDLGRWKKVGEILPMQECDAKGLAAPCAKVLGGRLHL
                     FYQTYGNGPRDAICVAVSDDGLRFTPHPSNPIFRPSGQWTVGRAIDADVISFKGRLFL
                     AYATRDPSMKIQMLGMATADPESDLGPTAWQQCGEGPILRPELPWEQDCIEAPSMVVR
                     DDKLIMFYAGAYNNRPQQIGVAASDDGLHWKRLFTEPLIPVGPPDAWNASESGHPGVF
                     VDEDGQTYVFYQGNNDQGRTWWITWDRVTWQGTIPYVIPAERQVSQK"
     gene            complement(9474..10943)
                     /locus_tag="PLD05_11075"
     CDS             complement(9474..10943)
                     /locus_tag="PLD05_11075"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOP78027.1"
                     /translation="MWRTLLYIPTHVGQMPVFGFGLLFWAWVILTIGLVWWQVRRHGW
                     TNELWSTVVLYGIVGVLIVLVLPRVTVDIPGLVDTKSQPVRGLPIRGYGSMLLLGVAS
                     AVGLAIYRAKRRGLSVEWVSNLALWCIIPGMIGARAFHVIEYWPIHYGPLWKTAGPIA
                     GLMAIVNVAQGGLVVYGSVIGGLLGIALYAWQQRTAFLSILDLLTPCFLIGLAFGRIG
                     CFLNGCCFGGVCELPWSVRFPAGSFAYLSQAEHGQLPIYGIKFKEDPRHPQRLPVILE
                     VAPQSAAARAGVKPGRHIAAINGLPLAGTEGESPTSRLVVQLIALYGDPVVLEGGRGC
                     VCSHGPNVIDISKISRSGVSSNATHLPASHSIMIRIDTAEGDHYQWETTEGLPARSLA
                     VHPSQLYSAANAAILCLFVLAYEPFRKREGELFAWFLILYPVTRFLLEIIRADEPGSY
                     GGLTIAQVVSLGLLVCGIATWIYLWRFTPGEQPNQAKSP"
     gene            complement(11059..11916)
                     /gene="panC"
                     /locus_tag="PLD05_11080"
     CDS             complement(11059..11916)
                     /gene="panC"
                     /locus_tag="PLD05_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOP78028.1"
                     /translation="MPLVLRSVKEMRAYSRSAKQSGSRIGFVPTMGALHRGHLSLVEA
                     AMKECETVVVSIYVNPTQFGRGEDFDRYPRNLEGDLNLLADYRVGAVFAPLDSEMYPE
                     GFDTWVQVGRIAQPWEGTCRPGHFRGVATVVLKLFAIVEPDLAYFGEKDYQQLLVVRQ
                     MVRDLNLPLEIRACPTVREPDGLAMSSRNQYLSPDERRDALVLFQSQQLAQELVNRGE
                     VDAAKITQAMREHILRVPYAKIDYVALVEPETLQEVTTVTRPVRAILAVRLGGTRLID
                     NMEIKPKTS"
     gene            12465..>13222
                     /locus_tag="PLD05_11085"
     CDS             12465..>13222
                     /locus_tag="PLD05_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOP78029.1"
                     /translation="MTTLSKNYFFGIGGKVVGPLDEKEIGRLIAEGIVNANTLAWCVG
                     QARWEPVGAIEELQQAFGPLLEGQQVGAATAATSPDVPPPLPEDQTVASTPLSHKAKV
                     QANTGHLSPLQSAAYSMVCWLYRPWRGRRSIIREFVDQDPTRAVPVAIGTMVVLGILF
                     VMTIGAMFPPEEPSRSLPSQPTAGANTLGANTVGAMPPGGQLSPNVFLPMIRAQQDIN
                     NTIDDVYRYNRDSFDRQCETYRRGTYDWYSSSND"
BASE COUNT         2742 a         3589 c         3993 g         2898 t
ORIGIN      
        1 ggagcagatt ttttagagcc gttccgtcca gactgggcgg aggtatcggc ggagacgtgg
       61 gccgtggttg agccggaact tcccggcacg tggcaggggg tcgaggaggt cattgccagt
      121 ttgagcgaga agcatcgccg cttcctgctt aagcagccca tgactccggt cggtctggga
      181 ttgacgcaga gcatccttcg gtgcgttgag gcaagagagc gctggccaga ggtaccgctg
      241 gtgatggatt tgtgcccgct gttgtaccat tgcccggcgg ataccggccc aatgctcttg
      301 gtcctcctgg gtttggcaga agagatgggc gctcgctgga tcaccaccac gcacgaggtg
      361 aataccgcga ggacctccgt ccgagagtgc cacgtcatcc gccgcctgac ccactgtgcg
      421 aaacagttcc acgtaacgtt acgaaatgtg gattcttcct ttgctatgtt gcgggatcac
      481 cagatcagcg atggcgatga gaagtatctc gaagagctcc gcgctcgatt gcgtgatccc
      541 tcgccgcggc tgtttgtggt gaaagatcag cttcgcagtt gggcgtttgg ccaggaactt
      601 caggccgaag acgcttatca gctctttgac aaaatcctcg ccgcggccga atcgcagggc
      661 cgagagattg atgtggacac ggccttttat ttgggctacg agttgtgcaa ggcctccatt
      721 gctcggcaac tggggaaaaa ctattgtcag gatgaagccc ttcattgggg tctgcacacc
      781 attccggaac cttcgccccg agaacggcgc atccagcggt tgaagcgacg tcgtggccgc
      841 gccggttgtt cagaaggcaa gaactaatcc ttgttcgatg tctcgctcgc tgcccctggt
      901 tgtcgaggtc ccctatgccg attggcggcg gagtgttgcc gtctttgccc agttaccttg
      961 gcttgttgtc tttgacagtg cccaggagga cccacggttg gggcgatttt cctttgtggc
     1021 ggctgatccc ttcgaacgtt ggcttgtcca ggccgatgcg tcaggccagg cagcgcgaga
     1081 ggcctttcag agtttggcgg aacggacacg ctgcttccgg caagtatctt gccctgattt
     1141 accgccgttt cagggtggtg ccgcaggatt gtggggctac gaactcaatc gggcatttga
     1201 ggacgtcccc gccgctcgga ttgatgacat gcgaacgcca cttttggccg tggggcttta
     1261 cgatattgtg ctggccgtgg atcacgagcg aggccgggca tggttaattt cccaggggtt
     1321 tcccgagacc gatcctgcga gccgggcacg ccgcgcccgc gaacgcctgg atttctttcg
     1381 agaccgactt cgagaaccag ccgaagcgaa tgggcggccg attgcttcga ctatggcgcc
     1441 acgggaggcc ttaccagggc aggtcgcggt tttccagtcg ctcactggcg agggtcctca
     1501 tttttggagt aatttttcgg aagaagccta cttggcggct gtttcgcagg tggtggagta
     1561 catccgagcg ggcgacgtct ttcaggtgaa cctgtcccag cggttggcct gtcccgccgc
     1621 aatagattcc ttgtcgctct acgagctttt gcgggaggag aatccggctc ccttcgccgg
     1681 ctattgtgac ctcgggcagt ggcagatcct ctcgagctcg ccggaacgct tcctccgcgt
     1741 ccgcgatcgc tgggtcgaaa cgcggcccat taagggaacc cggcgacgga cccatgaccc
     1801 cgacacagat cggcgaattg cccgggagct gctgcattcc caaaaagacc gggccgaaaa
     1861 tgtcatgatt accgacctgt tgcggaatga cctctcgcgg gtgtgtttgg cggagagcgt
     1921 ctgcgtctcg agtctctgtc atctggaaac ctacgaggca gttttccact tggtctcggt
     1981 catcgagggg cgcctgcggg aatcgtacga tgcctttgat ttgcttgcgg cgtccttccc
     2041 gggcgggtcg atcactgggg ctcccaagat tcgcgccatg gagattattg ccgaactcga
     2101 accccatgcc cgcggcgcgt actgcggctg cctgggctat gtgggtttta atggtgatgc
     2161 ggatttcaac atcctgattc ggaccctcac cgcggccaag ggctggtggg tgttttcggt
     2221 aggtgggggc atcctcgcca cctcgcgccc agaggatgag tacgcagaaa cctggcacaa
     2281 ggcggaaggg cttctgcggg cgattcgggc cgcctatcag cgtgggggat gaaccgatgt
     2341 cagaaccggc ttcactcctt cagttggccg gcgtcataga gttcccaaaa ttcggcgggc
     2401 ggcatgacgc ggtcggcaat tccccaatcc gactcgccaa aggccgtgcc aatctcgcgg
     2461 agcgtcggca tgttccgcac cacgtccatc cccgactcaa tcttcgacgg cgtgaagatc
     2521 agtcgttcca gccgtaacca gcgaaggggt gtgaggtcgg tgacctccga gcgggcgata
     2581 tggagacgct ttaacggcat gcccttgagc ggactgaggt cgcggacctt ggtccccgca
     2641 atcgtcaaac tcaccagcgg gacactccgc aaaggcgaaa gatccgaaac cggggtgtcg
     2701 ttaagccaaa gcgtctccag cggacatccg gccaaaggcg aaagatcggt caccttggtt
     2761 cggactaagt tcagttgctt taagggacaa ccggccaggg gcgaaaggtc gctgacctcg
     2821 gtttcgctga gggacagata ctccagcggc ataccggcca ggggacgcaa atccttgacc
     2881 tgagtgcggt ccaaggccat ggccgtcagc ggcagccccc gcaaggggct aagatcactc
     2941 accgagcatt gatacaggtc caacttgtgg attcgcagcc cggccaaggg tgaaatgtcg
     3001 tgcacttccg gatcattgat ggccactacg actgcgccgt cctgattttc aacaatgact
     3061 tcgccacgaa acgtgggatt cgccttctgc atggcggccg tcagctccgc gggaacaacg
     3121 tcgcgaaggc gtgacgcacg tggggtagcg gtcgcctcgg gcgtggcttg gtcgcccgac
     3181 tcggcagtgg tcgcggctgt ctgagagggt ttctcttcgc gggtcggttg cgactgctgg
     3241 gccggtggcc ggtgacatcc cacgaggagt agtataaaaa ccatggaaga aagactaagg
     3301 cttgccaacc atttccggat gattgtcgtc attgtcaaag gttacctccc catcgttgga
     3361 acgtagtctt gccgtggctc gccgtcattg atgacgaaca cctcccgtgg atggccttcg
     3421 ttcagtgagg tgcccacggc aaaggaagtg atcggatttg cgcttccgag actcgattga
     3481 cgtgtgatcg aggtgatacg caacatacag catcctccga ttttacctcc cggcggtagc
     3541 acgtccaaca cacagcatgg gcgagggcgt tcgttgattg tttggaggaa cgcgctttcc
     3601 tggcccaaat ctaaagcggc gcgccggcgt ccaccacgtt gcccattgac gtcccatttg
     3661 gagctatggc cgggacgttc gatgggcctc taggagcgga ccatcaattg gtaggggcaa
     3721 ccccctgtgg ttgcccgggc tagaacccgg aaggacgtat gccgcggcga atttttgact
     3781 tgacagagtt cgaaatataa gtataatcac atgtgattat ccataaatta aggttgacat
     3841 gatctcccga acagcaatcc acggactgag ggcgttaatg attctggcag aaatgccgcc
     3901 ggaccaatat gcgggaaccg cggaactggc caagcaaatc aaggcccctc gcaattacct
     3961 gggcaagctc ctgcagcggc tggcggacga gggattattg gaatcccagc ggggaaaagg
     4021 cggcggattt cgtttggccc gtccaccaga aaggatcacc atctacgacg ttgtcgagcc
     4081 cctggatcgc gtcagccgat gggagggttg tttgttagga cgatctcgct gttccgatga
     4141 ggccccttgc cccgtccatg tacgatgggc caaggtgcgg gccgtctatc tggaattctt
     4201 gcggacaaca acgcttgatg aattggtcgc gcaacgaagg gagtcgccaa gctgtagatc
     4261 atgatgttaa gtcgaggtgt ggtaaaggtg ttcagttttt ttgagattga ataggataac
     4321 cacatgcgat tcacttataa accagccaac aaggcttggc aaaccgtgat cgcggagtct
     4381 cgccaaagag ccaaaaacct tcccgtgggt tttggggctg cgggcagacc gagtgccgtt
     4441 tttggaacgc aaggcgtttc actgaacgat tctgggtgtt gcagtttagt tgaccttgat
     4501 aaggagagga tcaaatgaaa cctaccgata tcttgcgagc agaacatcga gtgattgagc
     4561 aggttttggc cgtgctggaa aagatggcca cccaggccga ggcgagcaaa tcggtcgatg
     4621 tcgaatccgc acgtcaggtg atcgatttta tccgacactt tgccgaccgt tgccatcacg
     4681 ctaaggagga ggatcggctc tttccggctt tagaagccag gggttttccc cgccagggtg
     4741 gcccgacggg ggtcatgttg gcggagcacg aaatggggcg ggaatgtgtt cgggatatgg
     4801 atcgggcact tggcgaacta gatcacgcgc cggaagcggc tttaagtacg tttgttacgg
     4861 cggcacggcg ttacgtcgct ctcttgcgcg agcacattgg aaaggaggat aacgtcctct
     4921 tcgtcctcgc cgagcgcacc atcgaggaag acgcccaggc acagctcctt tcggagtttg
     4981 aggcagttga aaaagagcac gtgggtgagc acgttcacga aaaatatgtt cagctcgccg
     5041 agagcctgat ggagcgatac ggaattgaca agcagagcat cccccgttcc ggcgacttct
     5101 tttgttgcca ctaggcgaac ccatgcataa gtcgcaaagg ggatatttga atcgcacatc
     5161 agcgagtgat ttgtgaaaag acattatcgc aaattgaaca gcgggtggcg gatgaagagt
     5221 ttccccatgc ccgcggtagg tgtcaggcgt aacggtcaac accagcatgg cacacaaggg
     5281 aacgaccaaa caggggttgg gatgacctaa acacggtgcg tgtgtatggc cctcgtagtg
     5341 ttgaaggtga gtgtaacaga gtgaccgaca gaaggaggtt gatgtcatgg gcatgcgaag
     5401 tttgcgaaat ggtgccgtcg tgagctttct ggtgggtatg gccctccttt tggtgggcgg
     5461 attcttcgcg gtggaccatg ttccgccgat tcccaagaag atcgagtggc aaggccaagt
     5521 gcttgctgac catgaggcca tcatgcgcgg tcaggatgtg taccagcgat atggattgat
     5581 ggaccacggc agtgtgtggg gccacggttc tttgcgggga atggattttt cggcgtacac
     5641 cctgcatctc ctcggttatt tggtgcggca gtggcattta ggaaacggca gatctgacga
     5701 gaatctttgg gagcaactgc cgcccgcacc gcgtcgggag ctcgatcaaa ccgacatgct
     5761 cgtcatccaa gagttgaagc ataaccgcta caatgcggag acaggcacgt tggaactcac
     5821 cgaggctcag gcatacgctt ttcggcaact ggaaaaattc tgggacaaga cgtttggcca
     5881 gggagattcc cattatggtt ttctgcccaa caccattccc tctgagcaag agcgacgtga
     5941 cattgcggcc tttttctttt ggactgcttg ggctgcggtg acgcctcggc cgggtctgga
     6001 ctacagttac accaacaact ggcctcctga tatcaccgtc ggtaaccgtg catcaagtga
     6061 ggcggtgctt tatagcattt tgtcggtcct ggcactcttt gtggtcctgg gccttgtggt
     6121 gtacattgtc catcggtacg agttctttta tggtgaatcg cgggccgtcg aggcggctta
     6181 ccgacttatg aagacgccgg taactcctag ccagcgggcc agcgctaagt tcttcttggt
     6241 ggccgggttg ctgtttattg tgcagatcct caatggtggc ctgcttgccc attacacggt
     6301 tcatccggga aagttctatg tcgaaattgt gggacaaatg tacccttata gctgggccaa
     6361 gacctggcac ctgcaattgg cggtgttgtg gattgctatt tcatggatgg gtacggccat
     6421 ttacctggcg ccgcttgtcg ggggtcgaga accgaagggc cagcgcctgc ttgtcaatat
     6481 cctgtttgtt gccgccgttg ccgtgacggt ggcaagcctg atcggtgagg tgctgggcat
     6541 taaaggttac ttgacgcact taggaccgag tgcttggttc tggttggggc atcaaggttg
     6601 ggaatatctc gaattgggac gactatggca gattctcctg tttggcggat tgatctactg
     6661 gcttgtgatc gtctatcgtg ccatcgcgcc aggcctacgc cgcaatcaga accaggcaga
     6721 cacagaggaa gatgcccacc ggcgggcact tatcctcttt tacctattta gcgggacatt
     6781 tgtcgtcctg ttttttgggt ttggtttgct ttacgggcga ggaacacatt tgacgatcgc
     6841 ggattattgg cgctggtttg tcgttcacat ctgggtggaa agtatttttg agttctttgg
     6901 ggtggccgtt atctcgctct ttctcgtgac acttggcctc gttaccgcta agtcggccct
     6961 gcgggttgcc tatctgacgg ccattctcgt ctttttgagt ggcatcccgg gaacggccca
     7021 tcactatttc tggtacggtg gaccgagttt ttggctggcc atcggtgggg tctttagctc
     7081 tcttgaaccc attccgctga tactcttggt cgtgcgggcg tggatggagt acaagtcgat
     7141 ccgtgaggca ggccacgagt tcccttatcg ttggccactc tatttcctca ccgcctcaag
     7201 tttttggaat ttcttggggg caggcgtctt cggcttcatg atcaacttgc ctttgatcaa
     7261 ctactacgag cacgccacat acttgaccag taatcacgga cacaccgcgc tcttcggcgt
     7321 gtacggaatg ctggcaattg ccgtgatcct tttctcttgg cgtgggctgg tcgagaactc
     7381 ggcatggaac gaccggattc tcaaaatcag cttttggggc ctgaatgctg gcctgttctt
     7441 gatgttcctc accgggttac tgcctatcgg tttgctccag gtttatcatg cctatgcgaa
     7501 cggcttttgg tggggacgaa gtgccgcctt ttacgagatg cccttgatcc aggccctcgg
     7561 taacttccgc atcatccccg acacaatcat tatcgttgtc ggtgccctgc cgttgctgta
     7621 ctttttgctg cggacgtttc cacggttacg cacggctcag gggtcggagc agggttgatc
     7681 gcccgcggat agagaccacc ttcttgggaa aactcagagc cagttggcca cgatggtcga
     7741 tgggcctcca gcagggggcc atcaattgct aggggcaacc cccccgtcgt tgcccattgt
     7801 tgaacccgga aggacgtgca ccgtcgtctc caaggtggct cagttataat gatctgctac
     7861 ggcgcaaccg gtgtgatccg aggcacatcg aatcttcgcg tgccccgtgg tgtttgattg
     7921 gcacggttgc gaggggtgcc agttcggcct tagggtggca ccgggcgagg cggtcaggag
     7981 aggggccgca atcgtgacgg ccacagtcgg tcgtaagagc ctatcccaaa aagtccccct
     8041 ctccctccgg gagagggttg gggtgagggc agggagggac gcggtccccc gcgtccgtca
     8101 tttagagcct atcccaaaag gtccccctct cccaccgaac gggcgagggg gagattatgg
     8161 gatgggttct aagtgtgtga ccacccacca gccgtgaatt tcattgtgag gagaatatgc
     8221 aactttggca ttcattgtct cagcttcgtt ggatcctgct gagctgcttg ggtgcgttaa
     8281 gcgttacagt gctgcttgag ccttcagaaa cttgtccggg tagcgccatg gctagggccg
     8341 aggcaacacc cagggccgaa ccggcggtgg aattatcgac cgattaccgc gacgaggggc
     8401 cgggatatat ctgcgagatc cggaacggtc ggacgttcta cggagatacg tcccggcggg
     8461 gagtgccttt tgccaaggat ccgtctgtgg tgcgatttgg ggaccggtat ttaatgtatt
     8521 attcgatccc gccgttcgct aaggaccgtc ctaataacgg ctgggccgtg ggaattgccg
     8581 aaagccgcga tcttggaagg tggaagaagg tgggcgagat cctgcccatg caggaatgcg
     8641 atgctaaggg cttggcggca ccgtgcgcga aggtcttggg cggtcggctg catctcttct
     8701 atcagacgta tgggaacggc ccgcgggacg cgatctgcgt tgccgtgtcc gatgatggcc
     8761 tgcgatttac gccgcacccc agcaatccga tctttcgccc tagcggccag tggactgttg
     8821 gccgggcgat cgacgccgat gtgatctcgt ttaaggggcg tctctttttg gcctatgcca
     8881 cgcgggaccc ctcgatgaag attcaaatgc tgggtatggc gacggccgat cccgaatcgg
     8941 atcttgggcc caccgcttgg caacaatgcg gtgagggacc gatcctccga ccagagcttc
     9001 cctgggaaca agattgcatc gaggcgccca gcatggtggt ccgagatgac aagctcatca
     9061 tgttttatgc cggggcgtac aacaaccggc cgcagcaaat cggggtggcg gccagcgacg
     9121 atggtctcca ctggaaacga ttattcaccg agccgttgat tccggtcggt ccgcccgacg
     9181 cgtggaatgc ttcagagtct ggccatccgg gtgtctttgt tgacgaggat gggcaaacgt
     9241 atgtgttcta tcagggcaat aatgatcagg ggcggacgtg gtggattacc tgggatcgcg
     9301 tcacgtggca ggggacgatc ccctatgtga taccggcgga gcgacaggtc tcccaaaagt
     9361 gagtgtctaa accgggatgt gcactccaaa ccacagcacg cgatggcgca atacgaagga
     9421 acgtcgcact ggctcgatca gggccaggac gcgatcgcct tagcgaagcg acattaagga
     9481 ctcttggcct ggttcggttg ctctcctggt gtgaatcgcc agagatagat ccacgtggca
     9541 atcccgcaca caagcagtcc caggctcacg acctgggcaa tcgtcaaacc accgtagctg
     9601 cccggctcat cagcccgaat gatttccaaa aggaatcggg tcacggggta gaggatcaga
     9661 aaccaggcaa aaagttcgcc ctcgcgctta cggaacggct cgtacgccag gacaaagagg
     9721 cacaagatgg cggcattggc ggcgctatag agctggctgg ggtggacggc caggctgcgg
     9781 gcgggcaggc cttcggtggt ttcccactgg tagtgatcac cctcggccgt gtcgatacgg
     9841 atcatgatgg agtgggaagc tggcaaatgg gttgcgttcg aggagacacc ggagcgcgat
     9901 atcttggaga tgtcaatcac atttggccca tgtgaacaga cgcagccgcg tcctccttcc
     9961 aagacgacgg gatcaccgta gagggcgatg agttgcacga cgaggcgtga ggtgggcgat
    10021 tctccctccg tccctgccag gggaaggccg ttaatcgccg cgatatggcg acccggcttc
    10081 actcccgccc gagcggcagc cgattggggc gcgacctcca ggatgacggg aagtcgctgg
    10141 ggatgccgag ggtcttcctt gaacttgatg ccatagatgg gcagttggcc atgctccgct
    10201 tgactgagat aggcgaacga tccggcaggg aagcgaacgc tccaaggcag ttcgcacacc
    10261 ccgccaaaac agcatccatt gagaaaacag ccgatccgtc cgaaagccag gccaatcaaa
    10321 aagcaaggag tcaaaagatc aaggattgac aaaaacgccg ttcgttgttg ccaggcatag
    10381 agggcaatac cgagcaatcc gccaatcacc gagccgtaaa caaccagtcc gccttgggcc
    10441 acattgacga tggccatcaa accggcgatg ggccccgcgg tcttccagag gggaccataa
    10501 tggatcggcc aatattcaat cacgtggaag gcccgggcac cgatcatccc cgggatgatg
    10561 caccacaggg ccagattgct cacccattcc acagacagac cacgacgttt ggcacggtag
    10621 atggccaatc ccacggcact ggcgacgccc agcagaagca tagagccata gccacggatg
    10681 ggcaggcccc gcacaggttg gcttttggta tcgacaagcc ctgggatatc gaccgtgact
    10741 cgcggaagaa ccaagacaat gagcacgccc acgatgccgt aaaggaccac cgtgctccac
    10801 agttcgttgg tccagccgtg acgacggacc tgccaccaga caagaccgat tgtgaggata
    10861 acccaggccc agaaaagtag cccgaaccca aaaacgggca tttggccgac gtgagttgga
    10921 atgtaaagga gtgttcgcca catgatcacg acaatccttt tgttgtgaac acccgaaaag
    10981 cggcggcctc ttgttgctgc ttggcttcgc tttggttccc accatcttgc gcgagtccgc
    11041 ccgccgcagg attttgcatt agctcgtctt gggtttgatt tccatgttgt cgatcaaacg
    11101 ggttcccccc aatcgaacgg ccaggatggc ccgcacggga cgtgtcactg tggtaacttc
    11161 ttgaagtgtt tcgggctcaa caagggccac gtaatcgatc ttggcgtagg gcacgcggag
    11221 gatgtgctcg cgcatagcct gcgtgatctt ggctgcatcc acctccccgc gattcaccag
    11281 ttcctgggcc agttgctggc tctggaagag caccagggca tcacgccgct catcgggaga
    11341 gaggtattgg tttcgggaac tcatggccag gccgtcgggt tctctgacgg ttgggcatgc
    11401 ccgaatctcc agcggcaggt tcaaatcccg gaccatctgt cggaccacca agagctgctg
    11461 atagtccttt tccccgaaat acgcaagatc aggttcgacg atcgcaaaca gtttcaaaac
    11521 cacggtggcc acaccgcgaa agtgtcccgg gcgacatgtt ccttcccacg gctgggcaat
    11581 acgaccgacc tgcacccagg tgtcgaagcc ttcgggatac atttcgctat cgaggggagc
    11641 gaacacggcc cccacgcgat agtcggcaag caggttcaaa tcgccctcca aattacgcgg
    11701 ataacgatcg aagtcctcac cccgaccaaa ctgggtgggg ttgacataga tcgatacaac
    11761 gacggtctcg cactccttca tggcggcctc caccaaactg agatgtcctc ggtggagggc
    11821 ccccatggtg gggacaaaac cgatacggct tccggactgc ttggcggacc gcgagtaagc
    11881 ccgcatctcc tttaccgaac gcaaaacaag cggcatggcc atctctagac ccctattcga
    11941 tatcgcaaga ggaatcaccg gccttcatgc acggtctctt gtcatcgcgg cgacaagagc
    12001 cattgctgga ccgtaaaaca tctactttac cacagatggc agtttgccaa gcaggtccca
    12061 gcggttcgct gcgttgctcc cgacaaagcc ggactgattt cgcggacgcc aacggccaca
    12121 cgcaaaccaa aaatgggcag ctatttcttg acattattac cccctcagcc gaggggcatt
    12181 tcgtgaccta agtttttatt agggccggta ctgtgtgacc ggtgcagtct tgcgcggatg
    12241 agtgagacct cgaggtaatc ggccttgtgt catcacggac cagccttctg caccaaatgg
    12301 cgcactgagc ctcgtttcac aatcgtcgat cacctggttg gaaaaagtct cgctgtcatc
    12361 ttccaaagga agcgcgcggg ctcgctgaga tcatccgata ttggcccacc ggttgtaggt
    12421 cttggcaaaa caccaaataa atcctcagta gcggagactc attcatgacg acactgagta
    12481 agaactactt cttcggcata ggaggcaagg ttgtcggacc gcttgacgaa aaggagatcg
    12541 gaaggctgat tgccgagggc attgtgaacg ccaatacgct cgcttggtgt gtggggcagg
    12601 cacgatggga gcctgttggg gcgatcgaag aactgcagca ggcttttggt cctctcttgg
    12661 aaggtcaaca ggttggggcg gcaaccgcag ccacatcccc ggatgttccg ccaccgcttc
    12721 ccgaggatca aacagtagct tcaactccat tgagtcataa agcgaaggtt caagcgaata
    12781 ccggccacct cagtccatta cagtcggcag cctacagcat ggtgtgttgg ctctatcgcc
    12841 cgtggcgagg acgccgttcc ataatccgag agttcgtgga ccaggacccg acacgtgccg
    12901 tgcccgtggc gatcgggacg atggttgtcc ttggaatact ctttgtgatg acaatcgggg
    12961 cgatgtttcc accggaagag ccatcgcgtt ccctgccttc acagcctaca gcgggcgcaa
    13021 atacactagg cgcaaatacc gtgggcgcaa tgccgccagg tgggcaatta agccccaacg
    13081 tgtttctgcc gatgatccgt gctcagcagg atatcaacaa cacgattgac gacgtctatc
    13141 gctataaccg tgactcgttc gatcgccagt gcgaaaccta caggaggggt acctacgatt
    13201 ggtacagctc gagcaatgac ta
//
LOCUS       DAOSUT010000118        13127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2813_length_13127_cov_9.749082, whole genome shotgun sequence.
ACCESSION   DAOSUT010000118 DAOSUT010000000
VERSION     DAOSUT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_13127_cov_9.749082"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLD05_11090"
     CDS             complement(<1..599)
                     /locus_tag="PLD05_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOP78030.1"
                     /translation="MAKDVFVRTKPHVNVGTIGHIDHGKTTLTGAILAVQSKKGLAKF
                     KSYADIAKGGTVRDATKTVTIHVAHVEYETEKRHYAHIDCPGHADFIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHVLLARQVNVPAIVVFLNKIDLVDDPELLDLVEMELRDLL
                     KKYDFPGDEVPIIRGDALSAYQNPDDPEKTKCIQELLDA"
     gene            complement(688..760)
                     /locus_tag="PLD05_11095"
     tRNA            complement(688..760)
                     /locus_tag="PLD05_11095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(725..727),aa:Thr,seq:ggt)
     gene            complement(847..917)
                     /locus_tag="PLD05_11100"
     tRNA            complement(847..917)
                     /locus_tag="PLD05_11100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(883..885),aa:Gly,seq:tcc)
     gene            complement(945..1026)
                     /locus_tag="PLD05_11105"
     tRNA            complement(945..1026)
                     /locus_tag="PLD05_11105"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(990..992),aa:Tyr,seq:gta)
     gene            complement(1090..1162)
                     /locus_tag="PLD05_11110"
     tRNA            complement(1090..1162)
                     /locus_tag="PLD05_11110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1127..1129),aa:Thr,seq:tgt)
     gene            1399..2082
                     /gene="tsaB"
                     /locus_tag="PLD05_11115"
     CDS             1399..2082
                     /gene="tsaB"
                     /locus_tag="PLD05_11115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOP78031.1"
                     /translation="MKTLAIETTAPVGSLAFFDDGQVLRHWTLPEDRRSAATLAAELQ
                     RALQAIGWRPNDVELVAVSLGPGSFTGVRIGLVTAKVFAYATGAQLIGVDSLDAVAEN
                     IPGTYSPLVVLADAQRGEITAKLYVWEPTAGWLITKQSGLVTPQDWLPHLALEHQFWL
                     AGPGLYRFKESIGDQYRMVPEAFWSPTAVGVGKLAWKRFLAGQTDDIWTLAPIYYRAS
                     YAEEKRAPR"
     gene            complement(2168..3451)
                     /gene="hemL"
                     /locus_tag="PLD05_11120"
     CDS             complement(2168..3451)
                     /gene="hemL"
                     /locus_tag="PLD05_11120"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOP78032.1"
                     /translation="MRWPLSEKAYELAKALIPGGVNSPARAFGAVGGHPLFIDRAQGA
                     WVWDIDGCRYVDYVGSWGPMILGHAHPEVLSAIESAMRRGTSFGAPTQAETELAELVC
                     KIVPSIEKVRLVNSGTEATMSAIRLARGATGRSKIIKFAGSYHGHVDSLLVAAGSSAA
                     TLGVPNSPGVTAGTAQDTLVLRYNDVEGVQQVFEKMGEEIAAVIVEPVAGNMGCVPAK
                     AEFLRKLRELTHKYGAILIFDEVMTGFRVALGGAQSLYGITPDLTTLGKIIGGGLPVG
                     AYGGKASIMDHVLPVGKVFQAGTLSGNPLATAAGIATLRILQRENPYPLLEARSHQLE
                     KGLTEIIAQTGLPARVQRCGSMLTLFFTSEEIIDWDTASRCNTKQFARFFWGMLEEGV
                     YLPCSQFEAWFVSTAHGETEIAKTLEAASKVLKSL"
     gene            3704..6088
                     /locus_tag="PLD05_11125"
     CDS             3704..6088
                     /locus_tag="PLD05_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOP78033.1"
                     /translation="MTELTRDEIAARYFEQLQYQPYPMQEEAMLAWFSARQGVLVCAP
                     TGTGKTLIAEAALFEALHTNTVAYYTTPLIALTEQKYREMQAAAVRWGFSARDVGLVT
                     GNRRENPDAQILVVVAEILFNRLLHPEIWDFSCVSAVVMDEFHSFNDPERGIVWEFTL
                     GLLPSHIRTLLLSATVGNAAEFLHWLRVSHGRDLELVVATDRKVPLVFQWVPDRLLNE
                     QIEEMTQGDEASRKTPALVFCFNREECWSIAEQLKGRDMITREQQAAIQEILKEVDLS
                     HGAGPKLKQLLLRGVGVHHAGVLPKYRRLVEDLFQKKLLSVVVCTETLSAGINLPARS
                     VVLPSLVKGPREKRRLIDPSSAHQMFGRAGRPQFDTQGYVFVLAHEDDVKINRWKEKY
                     DQIPEDTKDPQLLRMKKDLKRKMPQRRPDEVYWTEAQFWKLKNSPPTKLVSRGHLPWR
                     LLAYMLQASPEVEPIRRLVSKRLMESGRIASELRYLNRMLLTLWRAGFVELEPTPPAS
                     AFAVVPKKPSIDRSATSWEFPAKSTTDEEESSAQPESEGAETPEYQPVRAIPTEHIWK
                     ISVFRSVNPLYGVFLINQLGVANTQEILQAMESVLELPRSLMNYVRVPSYDDLPPGPL
                     ATTRLDPLLLELGLALPYQLTGRSEEEDEAEKEARRRQQRRGFVEDRPRVLTLAEKLE
                     LLFRYDFPDVFDIRITPVWAAGELLEYGDDFNKYITSKSLQKQEGIIFRHLLRLILLL
                     GEFKQICPVELTPEVWQAQLDELIVRFSEVCRRVDPTSTEQAMEQVESESVIDD"
     gene            6305..6667
                     /locus_tag="PLD05_11130"
     CDS             6305..6667
                     /locus_tag="PLD05_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78034.1"
                     /translation="MAQPDRSPHQPVETPSEAPQSPAGCLLRLYWMIGGYLIAVLCGV
                     SIVNHHGDFSLVDVIYWLAIIGILAARWVDIKRFSGTRADGQLATMRDWQTHAIVTVV
                     AGIIGWIIIHFVRWQGLW"
     gene            complement(6681..7241)
                     /locus_tag="PLD05_11135"
     CDS             complement(6681..7241)
                     /locus_tag="PLD05_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOP78035.1"
                     /translation="MDLLAVLFIALALAMDAFAVSLAVGLRLERITARHTFRIAFHFG
                     LFQFLMPILGWAVGRQFSLLISTWDHWLAFGILVLLGGKMIWQAFDDELTFTAEDPTR
                     GWSLVGLSIATSVDALAVGVSLAVLAGSVWFPAVIIGLVAAFLSLIGIHSGNWLGRRF
                     SQWGEICGGGILIVLACRILLSHLAL"
     gene            complement(7605..8210)
                     /locus_tag="PLD05_11140"
     CDS             complement(7605..8210)
                     /locus_tag="PLD05_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOP78036.1"
                     /translation="MQRLISVACLAVLSAGLLIFGSCAVAEQTGVNVGDKAPDWKNLI
                     GVDDKQHSLSDYADAKLIVLVFTCNHCPVAKAYEDRLIALQKDYQEKKVQVIALNVNN
                     IPQDRLDQMKIRAKEKGFNFPYLYDPTQKTGHDYGAKVTPHVFVLDSERKIAYIGAID
                     DNMDPKQVKRHYLREALDALLAGKTPETTMTKAFGCSIKYE"
     gene            8582..8752
                     /gene="rpmG"
                     /locus_tag="PLD05_11145"
     CDS             8582..8752
                     /gene="rpmG"
                     /locus_tag="PLD05_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOP78037.1"
                     /translation="MAKGGKKKKAEVVFLVCEETNDYNYTLRRKPGGEKLRLKKFCPR
                     CRRHTWHSEKKK"
     gene            8819..10573
                     /gene="recJ"
                     /locus_tag="PLD05_11150"
     CDS             8819..10573
                     /gene="recJ"
                     /locus_tag="PLD05_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOP78038.1"
                     /translation="MKKRWRISFHDPVAIADLARRAEIPPVVAQLLLSRGISDPSEAR
                     WFLKPSLQALHPPEAFPACEQAADIIWAAIQARQRIAIYGDYDADGITATAILWKCLK
                     LLGADVRYYVPNRLEEGYGLQTAAIEDLVQQGVQLIVTVDCGITAIPEVAFAHEKGIE
                     VIVTDHHEPGPDLPQAAAIVHPRIGSSPFPFPWLSGAGVAFRLAWAIARRASSGQRTD
                     ERLRDFLVQAVGLAAIGTVADVVPLWDENRILVHYGCTHSLPKRPTLGLSYLQSLASQ
                     SSNGKTDSEWIAFQLAPRINAVGRLGQAALAVELLVTEKEDRAAELASYINSLNEQRK
                     SLEQSIYLRALRQIKEQCDPDTDPAFVLADTDWHQGVIGIVAGRLAEKFHRPVILISL
                     SKTGMARAVGSARSVPGLHLHEALTACAHHLERFGGHSQAAGLTIAEQSIPAFRHEFC
                     QYVADHFDREQNLAEIHIDCEFPLGVFTVEVVKQIERLAPFGMGNPRPIMCCENVTLV
                     EPPRIVGQGQQHLSLRVHHSGTTMRAIAFGAGDRSHELSCGEPFDLAFRPVITEFRGF
                     ERVELHVVDWRTPPQRDG"
     gene            10652..12181
                     /locus_tag="PLD05_11155"
     CDS             10652..12181
                     /locus_tag="PLD05_11155"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOP78039.1"
                     /translation="MASTRLSPKIYRWLKAELEDWKAEGLITAEQAEQIANRYAASAV
                     PGARANLITMVFSTMAGALVIIGIILFISFYWQQLPATVQMGLLVGGTIGFNALGLLV
                     RRFVPEGRILSDAIFLMAAIFFGASVFLVPQIAGRTMRVADGMLLWTTGTILLVLVLD
                     HPLFYAGLSFLVIGSLATAMVEDAYHLERTLYAILTGAVLCPVMIWAYGGRSVLSCLA
                     VGVAFCWWLVSFAFLQGFELPGILVGTYLALVGTAVWVLSEWSQLASPMTQILRLLGA
                     VTTSLAYLVLSARHACYDDWQSQARYYPEKLFFPANYLQLIPILIFLAIILAEWFSSR
                     PSEQSPSHTKRFDKFLVPTVALVIALLILALSLSHFSVDASFPVVGTILGNVGAIFVA
                     SALIVLGVAYNQSERLFVGLGAFLLWLWMRWLDVFGANLLTGAVLFILSGAVFWIVVV
                     VWIRFKRSLLTPAPQYISATGIPRFLVAARNWSAQRRPLGLLLCIVIECGLLVFLYVF
                     M"
     gene            12397..12843
                     /locus_tag="PLD05_11160"
     CDS             12397..12843
                     /locus_tag="PLD05_11160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78040.1"
                     /translation="MNPCQTEWEQHFAIASTCEEASQLINTILDQLQRHGWREDDIFA
                     VHVALQESLSNAIKHGNRCDPSRTIYVTCRLTQQCIEISVKDEGAGFDPTKVPDPTLP
                     ENLDLPSGRGLRLITGFMDQVEFRNGGRQIVMRRCRRQSPEMQTRT"
BASE COUNT         2889 a         3730 c         3475 g         3033 t
ORIGIN      
        1 atcgcgtcca aaagctcctg aatgcacttc gtcttttccg gatcgtccgg gttctgatag
       61 gcggacaaag catcgccgcg gataatcgga acctcgtcgc ccggaaaatc atacttcttg
      121 agcaaatccc gaagttccat ctcgaccagg tccaacaact ctggatcgtc cacgaggtcg
      181 attttgttga ggaagaccac gatcgcgggg acattcacct gccgggcaag gagcacgtgt
      241 tcgcgtgttt gaggcatcgg gccatccgct gccgacacca caaggatggc cccgtccatc
      301 tgggccgcgc cggtgatcat gttcttaatg aagtcagcgt gaccggggca gtcgatgtga
      361 gcatagtgac gtttttccgt ttcatactcc acgtgggcca cgtggatcgt caccgtcttc
      421 gtggcatcgc ggaccgtccc acctttggcg atgtcggcat agctcttgaa tttagccaat
      481 ccctttttcg actggaccgc caaaattgca ccggtcagcg ttgtcttccc atggtcgatg
      541 tgaccgattg tccccacatt cacgtgtggc ttggttcgaa caaatacgtc cttagccatt
      601 cttgctgaat ctccgttctc tggttctaac aggaactaac ccaatatcgt cacttgacac
      661 gatttaccgg ccggctcaga agcaacgagc tgctgatggg acttgaaccc atgacctcgt
      721 ccttaccaag gacgcgctct accggctgag ctacagcagc caaggcccgg ccacgttccc
      781 gaaaatcccg taaccgggtt aacttctcag atcgaacgtc ccctccacga taaccgattc
      841 acctgaagcg ggtgagggga gtcgaacccc cgtcaatagc ttggaaggct actgctctac
      901 cgttgagcta cacccgcctc cgccacgtcg ttacctggtc aaattggggg gtgcaggatt
      961 cgaacctgcg aaggcagagc catcagattt acagtctgac ccctttgacc actcgggaaa
     1021 ccccccaaaa caaaaaacga ccaatgggcg aaaaccaaaa ttgtactaca tccgtacccc
     1081 tgcgcgaaga gctaacggtg ggactcgaac ccacaacctg ctgattacaa gtcagccgct
     1141 ctgccaattg agctacgcta gcctcgcata ccgacgtctg atttaatata ccggtctggc
     1201 caaactctgc aagccgaccc agcaaccggt tttgcagtcg cggttcccaa tcccattata
     1261 ccgattcgcc aggtgattca tcctcgggtg taagcctgcc tcttggtgca ccaattggtg
     1321 gcaaggcccc accgccagga cggggctcat tacgataggg ctgggattta tagacgtcac
     1381 gaaatttgtt tagcgcaaat gaaaacactc gcaatcgaga cgaccgcccc tgttggttcg
     1441 ttggcttttt ttgacgacgg acaggtcctc cgccactgga cgcttcctga agatcggcgg
     1501 tccgccgcaa cactggcggc agaattgcaa cgggccttgc aagcgatcgg ctggcggcct
     1561 aatgatgtcg agctcgtcgc ggtatctctg gggccgggct cgtttaccgg agttcgcatc
     1621 ggcttggtga ccgccaaggt ctttgcctat gcaacggggg ctcaactcat cggtgtggat
     1681 tcgctcgacg cggtggccga gaacatccct gggacatatt ctcccctcgt tgtcctcgca
     1741 gatgcccaaa ggggagagat caccgcaaag ctttatgttt gggagccaac cgctggatgg
     1801 ctgattacca agcaatcggg gctagttacc ccccaagatt ggttgcctca tctggccctc
     1861 gaacatcagt tctggctggc agggcccggt ttgtatcggt ttaaagagtc aatcggggat
     1921 cagtaccgca tggttccgga agccttctgg agcccaacgg ctgtcggtgt aggtaaactc
     1981 gcttggaaac gctttctcgc gggccagacg gacgacattt ggacgctcgc accgatctat
     2041 taccgcgcaa gttacgccga ggaaaagcgt gcaccgcgat aatcctacaa tagggccgct
     2101 ctttgttgct ccccggcttt gggcgtcagt caccagctca ttgcatcacc atcttggtgc
     2161 tgtgcgatta aagagatttc aagaccttcg aggccgcttc taaggtcttg gcaatctctg
     2221 tctctccatg agccgtggag acgaaccagg cctcaaactg gctgcacggt aggtaaacac
     2281 cctcttcgag catgccccag aagaaccgcg caaactgttt ggtgttgcag cgcgacgcgg
     2341 tatcccaatc tatgatctct tcgctagtga agaacagggt gagcatcgaa ccacatcgct
     2401 ggacacgagc cgggagacct gtttgtgcaa tgatctcggt taaacctttt tccaactgat
     2461 gggaccgagc ttctagcaaa ggataaggat tctctcgctg gaggatccgc aacgtggcga
     2521 tacccgccgc cgtggccaag ggattgccgc tgagtgttcc ggcctgaaac acctttccaa
     2581 cgggcagcac atgatccatg attgaggcct ttccgccata ggccccaacc ggcaatccgc
     2641 cgccaatgat ttttcccaac gtggtcaagt ccggcgtgat tccatacaaa ctttgagcgc
     2701 cccccaaggc aacgcgaaag cccgtcatca cctcatcgaa aatcaggatg gccccatatt
     2761 tgtgagtgag ttcccttagt ttgcggagaa actccgcttt tgctgggacg caacccatgt
     2821 tccccgccac gggctcgaca atcaccgcgg cgatttcctc acccatcttc tcaaaaacct
     2881 gctggacccc ctcgacgtcg ttataacgca gcaccagcgt gtcctgggct gtccctgcgg
     2941 tcaccccagg agagttaggc acacccagag tcgcggcaga actccccgct gcaaccagca
     3001 agctgtccac gtggccgtga taactcccgg caaactttat gatcttgctc cggccggttg
     3061 cccccctggc caaacgaatg gcactcatcg tcgcttcggt accggaatta acaagtcgta
     3121 ctttctcaat ggacgggacg attttacata ccagttcagc cagttcggtc tcggcttgag
     3181 tgggtgcccc gaagcttgtg ccgcggcgca tcgcggactc aatggccgac aggacctcag
     3241 gatgggcatg accgaggatc atcggacccc aggaaccgac gtaatcgaca taccggcagc
     3301 cgtcgatatc ccacacccag gcgccttgag cacgatcaat aaaaagggga tgacctccga
     3361 ccgccccaaa ggcccgggcc ggactattca ctccccccgg aatcaatgcc ttcgcaagct
     3421 cgtacgcttt ttcgctgaga ggccaacgca ttgctgactc ctacttacat tgccgggttg
     3481 aaatgttcgc gatcccttta gacataaaat aggtagcgag gttacgcaac accgcctcca
     3541 agcccaatgt tttcacgggc atcaacggcg tctcccgttg aagcaacctt tcggatcgag
     3601 catgatgaca gaattgtttt tctcacagga caaagcctta tttttccgca acaaaaaatt
     3661 gacaaactga cgagaaaact cgctgcccag acggacgaga ccagtgacag agctgacgcg
     3721 ggacgaaatt gccgcacgat actttgagca attgcaatac caaccctatc cgatgcagga
     3781 ggaggccatg ctggcctggt tctccgcgcg gcagggggtg cttgtttgtg cgcctactgg
     3841 aacgggcaag acgctgattg ccgaggccgc ccttttcgaa gctttgcaca ccaacacagt
     3901 cgcctattac acgaccccgc tcattgccct taccgagcaa aaataccggg aaatgcaggc
     3961 cgcggctgtc cgttgggggt tttcggcgcg tgatgtaggc cttgttacgg gcaatcggcg
     4021 tgaaaatcca gacgcacaaa tcctggttgt ggtcgccgaa atcctcttca accgacttct
     4081 tcacccggaa atctgggact tcagttgcgt cagtgccgtt gtgatggacg aatttcatag
     4141 cttcaacgac cccgaaaggg ggatcgtttg ggagttcacc ttggggcttt tgccctccca
     4201 tatccggaca ctccttcttt cggcaacggt agggaacgcc gcggagtttt tgcattggct
     4261 gcgggtttct cacggtcgtg acctggaact tgtggtggcc actgatcgaa aagtccccct
     4321 ggtttttcaa tgggtccccg atcgcttgct caatgagcag attgaagaaa tgacccaggg
     4381 cgatgaggcc tctcggaaaa cgcccgcgtt ggtgttttgc ttcaatcgtg aagaatgctg
     4441 gtccatcgcc gaacaactca aagggcggga catgattacc cgcgagcaac aggctgcgat
     4501 tcaggagatt ctaaaagaag tggacctttc gcacggtgcc ggccccaagc taaagcagct
     4561 cttattgcgg ggtgtaggtg tccaccatgc tggcgttctg cccaagtacc gcaggctagt
     4621 ggaggatctc tttcagaaaa aacttctctc tgtcgtggtc tgcacagaaa cattatcagc
     4681 cgggatcaac ttgccagctc ggtccgtggt tcttccatct ttggttaaag gtccgcgcga
     4741 aaaacggcgt ctcattgacc ccagctcggc ccaccagatg tttggtcgag caggccgccc
     4801 tcagttcgac acgcaaggct atgtgtttgt cttagcacac gaggacgacg tcaaaatcaa
     4861 ccgctggaag gagaagtacg atcaaatccc ggaagacaca aaggatccgc aactcttgcg
     4921 gatgaagaaa gacctcaagc gaaagatgcc tcaacgtcga ccggacgagg tctattggac
     4981 cgaggcacag ttctggaagc tcaagaattc cccgccaacc aaacttgtca gccgtggaca
     5041 cctaccctgg cggctattgg cctacatgct ccaggcatcg cctgaggtgg aacccatccg
     5101 tcgtctcgtc agcaaacggt tgatggaatc gggccgcatt gcctcagaat tgcgatacct
     5161 caaccgcatg cttctgacgt tgtggcgagc ggggtttgtt gaactcgagc caactccgcc
     5221 agcatcagcc ttcgccgtag tgcccaaaaa gccgtccatt gatcgctccg ccacatcctg
     5281 ggaattccct gccaaatcca ctaccgatga agaggagagt tcggcacaac cagaatcaga
     5341 gggggctgaa acgccggaat accaaccggt gcgggccatt ccaacggagc acatctggaa
     5401 gatttccgtc tttcgcagtg tcaatccgtt gtacggagtc tttctcatca atcaacttgg
     5461 ggtcgccaac acccaagaga ttctccaggc catggagagc gtcctggaat taccgcgctc
     5521 gctgatgaat tatgtccgtg tgcccagtta tgacgatctc cctccgggac cactagcgac
     5581 cacgaggctc gatcccctcc tcctggaact ggggctagca ctgccgtacc aattaaccgg
     5641 tcgcagcgag gaggaagatg aggcagaaaa agaggcccgc cgacggcaac agcggcgggg
     5701 gttcgtcgaa gatcgtcctc gcgtcttaac acttgccgaa aaactggaac tcctttttcg
     5761 gtacgatttc cccgatgttt ttgatatccg catcacaccg gtttgggcag ccggtgaact
     5821 tctcgaatac ggggacgact tcaacaagta cattacaagc aagtccctgc aaaagcagga
     5881 gggtatcatt ttccgtcatt tgctgagact gatcctcctg ctcggggaat tcaagcaaat
     5941 ctgcccagtg gaattgactc ccgaagtctg gcaggcccaa ttggacgagc tgatcgtacg
     6001 atttagcgag gtttgccgcc gcgtcgatcc gacaagtaca gaacaagcca tggagcaggt
     6061 cgaaagcgaa agcgtgatcg acgactagcc acagttaaca acaatcaagg cgattcccca
     6121 gcgttacgga aactccctta ggcatgcttt gttagctcgg ttgttcttgt catacggcct
     6181 caaagccttt atcataggat caatcaacgt tcgacagtgc aagggtgcag catctagctg
     6241 caggtgttgc ggatggtcgg gccgccattc gtttttttct tcgagttaat gaggaaggaa
     6301 cgcgatggca caacccgatc gttctccgca ccaacctgtg gaaaccccct cagaggcgcc
     6361 acagagcccg gccggttgtc ttcttcgact ctattggatg atcggtggct atctgatcgc
     6421 cgtcttgtgt ggcgtctcga ttgtcaacca ccacggtgac ttttctctgg tggatgtgat
     6481 ttactggttg gccattatcg gcattttggc tgcccgctgg gtggatatca aacgattcag
     6541 cggcacccgg gccgacggtc aattggccac gatgcgggac tggcagaccc acgctatcgt
     6601 aacggttgtc gccggaatta tcggttggat catcatccac ttcgttcgct ggcagggtct
     6661 ttggtaactt gctgggtcaa ttacaaagcc aaatgggaaa ggaggattcg gcaggccaag
     6721 acaataagaa tgccgccccc gcagatttct ccccattgag aaaaacgccg tcccagccaa
     6781 ttgccactgt gaataccgat caagctgagg aaggctgcaa ctagtccgat aatgacggcc
     6841 ggaaaccata ctgatccggc aagcaccgct agagataccc caacggccag ggcatccaca
     6901 ctggtcgcaa ttgacaagcc tacgagtgac cacccccggg ttgggtcttc ggcagtaaat
     6961 gttagctcgt catcgaacgc ctgccagatc atctttcccc ctaggagtac caggatgcca
     7021 aaggccagcc agtgatccca ggtgctaatc aatagggaaa actgtcggcc aacggcccag
     7081 ccgagaatag gcatcaggaa ctgaaaaagc ccaaaatgaa aggcaatccg aaaagtgtgt
     7141 cgagcggtaa ttcgctccag acgcaaaccc acggccaagg atacggcaaa ggcatccatg
     7201 gccagggcta aggctataaa caaaacggcc agtaagtcca cttgctgcta ggaatctcgg
     7261 agtaataacg gcatcacggt acatgcggag aatacggctc cagccccgac tgggctattc
     7321 cttccacgta taggcaatta tctggagatc gacaacgccc tcaagccgaa ttggcaaaag
     7381 tggagtcctc aatcaccagc atgcactcat ctacgttcac gggcggagga agccagccct
     7441 cggttctgaa agcaccaagc cgccccgctg gcaaaaaata agcctcacat cgcttggagg
     7501 atgcgaggca aaagatcatt cggccaattc tgacattctc cctatcggga gaacaacggg
     7561 gacagttacc ggcctttttc ccggcgcagt ctgtgccaca aagatcactc gtactttatc
     7621 gagcagccaa aggcctttgt catggttgtc tctggggtct tgcctgccaa gagggcatcg
     7681 agggcttccc tcaggtaatg tcgcttgacc tgcttgggat ccatgttgtc gtcgatggcc
     7741 ccaatatagg caattttgcg ctcagaatcg agcacaaaca catggggcgt aacctttgca
     7801 ccgtagtcgt gccctgtctt ctgggtcgga tcatacaaat atggaaaatt gaagcccttc
     7861 tccttcgctc gaattttcat ttgatccaat cgatcttgtg ggatgttgtt aacgttcagc
     7921 gcgataacct gaaccttctt ttcttggtag tccttttgca gggcaatgag ccgatcttcg
     7981 taggccttgg ccaccgggca atggttgcag gtgaagacca aaacgattaa tttcgcatct
     8041 gcataatcag agagcgaatg ctgcttgtca tccaccccga taaggttctt ccaatcgggg
     8101 gccttatcac cgacgtttac ccccgtttgt tcggctacgg cacaagatcc gaaaatgagc
     8161 agtcccgccg acaacaccgc caaacaagcc acagaaatca gtcgttgcat tattcgacct
     8221 cccatcacta tgcgctaacc cttgcccgtc cctgacgctc acccaactgc tcgcgtccca
     8281 cagggatggg ccaaagaata gtttaccgaa ctcgcccaga aggcaacccc acccagagag
     8341 ggtcagccct ctcttctcgt tcaggggagt tcataaaccc gttgtcaacg cggactatgt
     8401 caagcatatc ccaaagaatt tagggcacat cccattaccg tttggacagc tttgcgttac
     8461 aatccgacga tataggtttt gacttgcaca accacccggt gattgagcgt agccgcgtgg
     8521 gtggggtatc ggttcgagag aactccaagc gattggtttc tgaaacgtcg ggaaacacac
     8581 tatggcgaaa ggtggaaaaa agaagaaggc tgaggttgtc tttctggtct gcgaagaaac
     8641 gaacgactat aactatacgc tccgtcggaa accgggtggg gagaaacttc gcctcaagaa
     8701 gttttgtcca cgctgccgcc ggcacacatg gcattccgag aagaaaaaat agcctgtcga
     8761 tggcgtaaca ttaacgctcg ctgggattgc cattcgcggg cgatgaggct ccggggctat
     8821 gaaaaaacga tggcggatct cttttcatga tcccgtagcc attgccgacc ttgcacgccg
     8881 agcggaaatt cccccggttg tcgcccagtt gcttttgtct cgcggtattt ccgacccctc
     8941 agaggcccgc tggtttctca agccgtcact ccaggctctc caccctccgg aggcttttcc
     9001 ggcttgtgaa caggcggccg acatcatttg ggcagctatc caggcgcggc agcggatcgc
     9061 catctatgga gattacgatg ccgatggcat cacggcgacg gcgattcttt ggaagtgcct
     9121 gaagttactt ggtgccgacg tccggtatta tgtccccaat cgtctggaag aaggctatgg
     9181 acttcaaaca gcggcgattg aggaccttgt ccagcagggt gtccaactga ttgtcacagt
     9241 ggattgtggc atcaccgcca ttccagaagt cgcctttgcc catgaaaagg gcattgaggt
     9301 catcgtcacg gatcaccatg agccgggtcc cgacctaccg caagccgccg cgattgttca
     9361 tccccgtatt ggctcatcgc cgtttccctt tccctggttg agtggcgccg gggtggcctt
     9421 tcgcctggct tgggcaattg cccggcgggc cagttccggc caacggacag atgaacgtct
     9481 ccgtgacttt ctcgtgcagg ccgttggatt ggctgccatc gggacggtgg ccgatgtcgt
     9541 accgctatgg gatgaaaacc gaatccttgt ccattatgga tgtacccata gcttgccgaa
     9601 gcgtccgact ttgggattga gctacttgca aagtcttgcg tcacaatcgt ccaatggcaa
     9661 gaccgacagt gagtggattg cctttcagtt ggcaccgcga atcaacgccg tcggccgatt
     9721 gggacaagca gcgttagccg tcgagttgct ggtgacagaa aaggaagatc gggcggcgga
     9781 gctggcaagt tacatcaaca gcctcaatga gcagcgaaag tctctcgagc aaagtatcta
     9841 ccttcgtgcc ttacggcaga tcaaagaaca gtgcgatccc gataccgacc ctgcttttgt
     9901 tcttgccgat accgattggc accaaggagt gatcggaatt gtggccggac gactggcaga
     9961 aaagtttcac cgaccggtga ttctcatttc gctcagcaaa actggcatgg cgcgggccgt
    10021 gggttcagcc cggagtgttc cgggattgca tctccatgag gccctcaccg catgtgccca
    10081 ccacctcgag cgttttgggg ggcattctca ggctgccggg ctgactattg cagagcaaag
    10141 catccccgct tttcggcatg agttctgcca gtatgtcgcc gatcattttg accgagaaca
    10201 aaacctcgcc gagatccata tcgactgcga atttccgttg ggcgtgttca ctgttgaggt
    10261 ggtgaaacaa atcgaacgcc tggccccgtt tggcatgggc aatccgcgac ctatcatgtg
    10321 ctgtgagaac gtgacacttg tcgaaccacc ccgcattgtg ggacaaggtc aacagcatct
    10381 ttcgcttcgg gtccaccact ccgggacaac catgagggcc atcgcgttcg gggccggtga
    10441 tcgaagccac gagctctcgt gcggagaacc gtttgatctt gcctttcgcc ctgtgatcac
    10501 cgaatttcgg ggttttgagc gcgtcgaatt gcacgtcgtt gattggcgaa caccgccgca
    10561 gcgcgacggt tgatcgccaa tcctcgaagg tgtatgctct cgtaaagctc cgcgttactc
    10621 gtcaacgaaa agaagggatg gaacgtactc cgtggcaagc accagattgt cacccaagat
    10681 ataccgctgg ctgaaggcag agcttgaaga ctggaaagcc gagggtctta tcacagcgga
    10741 acaggctgag caaatcgcca atcgctatgc ggcttccgcg gtccccggtg cccgggcaaa
    10801 cctcattacg atggtcttct cgaccatggc cggggcattg gtcattattg gcatcatcct
    10861 cttcatttcg ttctattggc agcaattgcc agccaccgtg cagatgggac tgcttgttgg
    10921 cggcacgatc ggtttcaatg ccctgggtct ccttgtccgg cggtttgtcc ctgagggccg
    10981 catccttagt gatgcaatct tcctgatggc tgccatcttt ttcggggcat ctgtctttct
    11041 tgttccccaa atcgccggga ggacgatgcg tgtcgccgac gggatgttgc tctggaccac
    11101 cgggacaatc cttttggtgc tagtgcttga ccatcccctt ttttacgccg gcctgagttt
    11161 tcttgtgatc ggttccctgg ccacggctat ggtcgaagat gcctaccatt tggagcgcac
    11221 gctgtacgca atcttaaccg gagccgtctt atgccccgtc atgatctggg cctatggggg
    11281 acggtcggtc ctgtcatgtc tggctgtagg cgtggcgttt tgctggtggc ttgtcagttt
    11341 cgctttcctt cagggatttg agcttccggg tattttggta ggcacttact tggcgcttgt
    11401 cggcacagcg gtctgggtgc tctcggaatg gtcccaactt gccagcccaa tgactcaaat
    11461 acttcgctta ctcggggccg tgacaacttc ccttgcttac ctagttttga gtgcccgaca
    11521 tgcgtgttac gacgattggc agtcgcaggc ccgttattac ccggagaagc tcttcttccc
    11581 cgccaattac ctacaactca ttccgattct tatattcctg gcgattattc tcgccgaatg
    11641 gttctcctcc cgcccttcgg aacagtcacc gagtcacaca aagaggtttg ataaattttt
    11701 ggtacctacg gtagctcttg tgattgccct actgattctt gcgctatcgc tcagtcactt
    11761 ttctgtggac gccagtttcc ccgttgtggg aacgatcctg ggaaacgtag gggccatctt
    11821 cgtggcaagc gccctcattg tcttgggcgt ggcctacaac caaagtgagc gcctcttcgt
    11881 gggcctgggg gcgttccttt tgtggttatg gatgcgttgg ctagacgttt ttggagccaa
    11941 cctcctcaca ggcgctgttc tgttcatttt gagcggtgcc gtgttttgga ttgtggtcgt
    12001 cgtttggata cggtttaagc ggtcgttact caccccagct ccacagtaca tatcggccac
    12061 ggggataccg cgattccttg tcgccgctcg caattggagc gcccagcggc gaccattggg
    12121 cctgttgctc tgcattgtta tagagtgtgg actattggtt ttcctatatg tttttatgta
    12181 aaccaggcgc gtatctcttg gagatgacaa ctgtctcgat ccgtcaataa gcgggctgat
    12241 gtgtgtcaaa tgcaaacaga ttcattcgca ggatttgctc acccgactca aacctgtctg
    12301 gcttttcttt ttgtcagacg atatattggg gcagccgtgc cgtgtgagaa aaggagcatt
    12361 tttttgggtt gaccccgttg catcgacatg tcactcatga acccgtgtca aaccgagtgg
    12421 gaacaacact tcgccatcgc cagcacgtgt gaggaagcgt cgcaactcat caacactatt
    12481 cttgaccaac ttcagcgcca cggttggcgg gaggacgaca tttttgcggt ccacgtggcc
    12541 ttgcaggaat ctttgagcaa tgccattaag catggcaatc gttgcgatcc ctcacgaacg
    12601 atctacgtga cctgtcgatt gactcaacaa tgcatcgaga tctcggttaa agacgagggc
    12661 gcggggtttg accccaccaa ggttcccgat cccaccctcc ccgaaaacct ggacctcccc
    12721 agtggtcgag ggctgcgact catcaccggt tttatggatc aagtcgaatt ccgcaatggt
    12781 ggccgacaaa ttgtgatgcg tcgttgccga cgccagtctc ccgaaatgca aacccgaact
    12841 tgagctgcgg tcgatctttg cacaattccc gcggaatcga tcttcttgcc atatggactt
    12901 atgagcctat cccataatct tccctcgccc cgtcggcggg agatgtgtgg cgggtgtggg
    12961 tgggtcgaat cgctgccggt tttctcaatc gattccgacg ttcgcttgcc ctcaccccaa
    13021 ccctctcccc gagggagagg gggacttttt gggatagact cttatatgag atcgccattg
    13081 tttttccggt acagccggcc ccaccctgtg gcggccgtgt ttcccga
//
LOCUS       DAOSUT010000119        12972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2849_length_12972_cov_9.337153, whole genome shotgun sequence.
ACCESSION   DAOSUT010000119 DAOSUT010000000
VERSION     DAOSUT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_12972_cov_9.337153"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLD05_11165"
     CDS             <1..553
                     /locus_tag="PLD05_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78041.1"
                     /translation="CPHPNPLPEGEGDFLRQALNSRRPPPKTAGKPSECDPEKQADVI
                     ATAREATDSTEVEPLLVHEVNGLLELNWQPLFQSVATAMSRGMSRELLALAFHVRLVD
                     SVFQLYQMLRCRSLVISGGCFQNQLLRELIGAEAKRFGVPLYQPQLIPAGDGGIALGQ
                     LWLTARTLQGLVRADSLCEYIRP"
     gene            582..851
                     /locus_tag="PLD05_11170"
     CDS             582..851
                     /locus_tag="PLD05_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOP78042.1"
                     /translation="MCLGIPGRILDKYERNGLLYGRVDFGGIAKEICLAYVPEAKPGE
                     YVIVHVGFAISRLDESEAMEQLALLREIDAAGEAAEEPQDMEKKA"
     gene            848..1942
                     /gene="hypD"
                     /locus_tag="PLD05_11175"
     CDS             848..1942
                     /gene="hypD"
                     /locus_tag="PLD05_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOP78043.1"
                     /translation="MRFISEYRDSTAVRHWLRELQNLVTRPWTIMEICGGQTHSFLRF
                     GLDELLPKEITLVHGPGCPVCVTPVELIDKAVAIASRPEVIFCSFGDMLRVPGTKDDL
                     LRVKSRGGDVRIVYSPLDAIDLAKQHPDRQVVFFAVGFETTAPANAMAVLQAAEAGLK
                     NFSVLVAHVRVPPAIEAILSSPRNHVEGFLAAGHVCTVMGTEEYLPLADRYHTPIVVT
                     GFEPADLLQGLAMCVAQLEAGKAEVEIQYSRSVRPEGNIVARRNIDQVFRIVDRKWRG
                     IGMIPQSGLALRDEFLDFDAEQRFATADVTAEESPECIAGEVLQGHKKPMACPAFGTR
                     CTPAHPLGAPMVSSEGACAAYYRYRRHEAG"
     gene            2509..3465
                     /locus_tag="PLD05_11180"
     CDS             2509..3465
                     /locus_tag="PLD05_11180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOP78044.1"
                     /translation="MDKVKIAAEFDPTADFVLYEGNCLDLLPRIPDGFVKLVVTSPPY
                     NLGKPYETRLDMDAYLSQQRRVIEECVRVLDDQGSICWQVGNYVDNGEIIPLDIVLYP
                     IFASLGLHLRNRIVWHFGHGLHASKRFSGRYEVLLWFTKGDEYTFKLDAVRVPQKYPN
                     KKYFKGPKKGELSGNPLGKNPGDVWEIPNVKANHVEKTIHPCQFPVELIERLVLAMTD
                     KGDWVLDPFIGVGTTAIAALMHGRKAIGAEIMPEYVEIAKERIRLAERGELRIRPMER
                     SVYDPDMPDASIPPQVVRLGSARTELPLFEQIARYSAQEQEE"
     gene            3462..4013
                     /locus_tag="PLD05_11185"
     CDS             3462..4013
                     /locus_tag="PLD05_11185"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOP78045.1"
                     /translation="MRIVYEYSHLGGAEILKVHYKEHEDEIKEIIAGVKASKTKISKE
                     KTMQGRELYSPKDMNQQFREAFAARGYAELRDTYTITIPNSDVAIPGAFKQIDFVKGK
                     VLVEVQFGKYAFMFYDMAKFQYFFNENKADVGVEIVPCHALHKQMSTGVSFGEQLVYD
                     IERLKRHFPAVPVKVILIDADTN"
     gene            4523..5605
                     /locus_tag="PLD05_11190"
     CDS             4523..5605
                     /locus_tag="PLD05_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOP78046.1"
                     /translation="MIQDKRSNIGIVGVAMLVLLLPLGGLAETIPSPLPDPDGKPADM
                     SQPVQVFILLGQSNMIGFGKVSGGEGSLEYAVKEKGLYPYLIDDAGNWTVRYDVRYVR
                     VMGSGTGPIKVFNNEWLTVSGRTIGPEIGIGHYLGEALAAPVLLLKSCIGNRSLGWDL
                     LPPGSKQYEYKGKIYAGYKESPMSWDKGTKPEPIGWYAGMQYDGDIARAKMVLEELDK
                     YYPGAKSYEIAGFFFWQGDKDRYNEAHAVHYEENLVRLIEQLRRDFNAPNAKFVCATL
                     GQTQKGATGNDGLILEAQLAVDGRSGKYPQFQGNVATVYTYPLSKGGASNNHYGGNAE
                     TYMNVGEAMGKAMVELLNEAKKDPLP"
     gene            complement(6062..7690)
                     /locus_tag="PLD05_11195"
     CDS             complement(6062..7690)
                     /locus_tag="PLD05_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOP78047.1"
                     /translation="MMRHHFQLSAPTWGMSAILGLFLTTWANAAPAFVSGTPTAPHRG
                     VWEGELTLDRVRGNPFFDVEFHIIFTLPDGSEVKAEGFFKGENRWAARAYCSQLGRWK
                     WRSVSNQPELDGRSGSFEVQPSNLPGKLRKHPADSRQFAYDNGQWFLHLGDTAYRFVA
                     ATEPLWQQYIDEAVEVGFTKVRTWFSSGRHDIGALFAPDRQGLDLAYWDEIERRLRYA
                     LEKYPHIQFQVIIYGEDWPELARYGKGDRAAQLVARYAQARFSAFPNVYWCISNDAHI
                     SSGPGPRNADPAVINQIGDDMKRREPWGTLLTNHQQRFQGYSFVNAAWSDIVTLEDRD
                     QVAGAIVLQYRRLTNCPVVLDEDRYGIYISPQHDRYFFRRLMWANLLSGGQATYGGLN
                     TYEPFDGPKGIRGIQGYRTAVADGRLDDGAADFLHIKRFFEESGLTLVGLEPNDAMAG
                     NNGHLVKVMEGKKTIIAYLQNPDSPTPETANVSETPATCRMHLPPGQWRIRWYDPRSG
                     KWHPDPERAEITGGYTRDFTSPFSGDAVLLLQLP"
     gene            complement(8011..9582)
                     /locus_tag="PLD05_11200"
     CDS             complement(8011..9582)
                     /locus_tag="PLD05_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOP78048.1"
                     /translation="MIRRTWVAFLLVVVNTANLAARECLASVFDVRDFGAVGDGVTLD
                     TKAIQAAIDHCAAEGGGRVLLTGKRVYLSGTVVLKSGVTLWIDSGTVLRASLDKDDYP
                     DFTPQLKYVYRDRFTRQLIYAEKQTNIGIAGPGTIDGQGTNEAFDARANPGDRDRPYI
                     LRFAECTGVRVREVTLLESARWCAHFLGCDDVLIDGVTILSSKRENRDGLDIDSCHRV
                     RVANCRVETGDDAIVLKATAHRPCKNVTVVNCHLSSQAAAFKLGTESNGGFEDIVMSN
                     CTIYDTGGAGISLLMVDGGVLDRVSISNVVMDNVASPIFIRLGNRARPLPDEEPPGQG
                     MMRNIVISGIQARGAGVLGCAISGIPGACIEHLTLRDIRIQSAGGGTVENGRATVPER
                     EAAYPSPRIFGPLPAYGLFIRHVRHLTLDGVELSFDTARGEERPALVLEDVHDAYLTK
                     LRAAAVSAMPTVRMVNCQGVLLHGCRAEQVSTPFLSVEGESTRDIAVVGNDFSGTQTA
                     MIVDPLVPESAVRQR"
     gene            complement(9624..10943)
                     /locus_tag="PLD05_11205"
     CDS             complement(9624..10943)
                     /locus_tag="PLD05_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOP78049.1"
                     /translation="MLVTASGKPFFWVADTAWQLIHDLDEAEMRRYFADRREKGFTVI
                     QTVVLVELRSDKPNAFGHFPIEPRRPDRPIVEPGPNNDYWDDVERVLRLAEENNLYVA
                     LLPTWGKWVISNWQDGIVDGFFTTENAEIYGRFIGNRFRDHNNIIWVLGGDKASPTDE
                     AKAIWRAMARGIAIGVTGEEDYSKVLMTYHTSGPGSTAWFLNKEPWLDIHGLQSGHGR
                     WALNWMMVEYAYTMRPTLPVIDLETSYPGFRHGRPPTVATDDDARRAAYWAVFAGAAG
                     HTYGHHSIWQMHSPKYPGVAGPKEYWYEALDAPSAFQMGYLRRLIESRPFLTQRPDLS
                     LLAFEQTKPWEMCLALRGDGYALVYTPTGRTLEIALDKLGFANVKAAWFDPRTGNTSE
                     IGQFPARGRRSFDPPGEPGEGNDWVLILDDAARTFPISDLKPGPKKP"
     gene            complement(11134..11454)
                     /locus_tag="PLD05_11210"
     CDS             complement(11134..11454)
                     /locus_tag="PLD05_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78050.1"
                     /translation="MQLGRWTFFAVTYDATQPRDNVRWYFSEPRESPGQAAIVLDRIT
                     SYNVGPVDMDIGPLAIGNFNKTMHGYGLDRQFRGEIRGLKIFGSRVSGRGALPLEVIR
                     EHQR"
     gene            complement(11464..>12972)
                     /locus_tag="PLD05_11215"
     CDS             complement(11464..>12972)
                     /locus_tag="PLD05_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOP78051.1"
                     /translation="FWRHLERRILDLQRLGIEADLILWHPYDRWGFAEMGRENDDRYL
                     RYCIARLSAFRNVWWSLANEYDLMAPGAARGHRGDKTMADWDRFFQILQKEDPHQRLR
                     SIHNCRVFYDHTKPWVTHASLQSSDMNGGVRFRTQFGKPVIYDECRYEGDIPQGWGNL
                     TPREMAQRFWLGTLSGCYVGHGETYKHPQDILWWSKGGVLHGQSPARIAWLKQFLTQC
                     PAFDELQPMGDDRGRFLLAKPGEFYLVYCVDQRKQVINLAEGHRYKVDLIDPWEMTIT
                     ALGTAEPGEFAVLPPRADVAFRFVAYQPGEPIRPTVRIEASATEGPAPLEVEFSSPTV
                     GKLRWDFGDGTTGEGPQVRHVFQSPGVYCVALTATTAEGQSATAFQEILVDHDPNVPI
                     VRVGFAGGEVPTLTFQGTAKRGADGSLLLPPGPPWGWIEVGDDVVEGLRGLRSFTILG
                     WVKPESLQVGSGGNRIVFCLKENRSGIDLVCHADGRLRLAVNKWPDSVQNDS"
BASE COUNT         2969 a         3660 c         3558 g         2785 t
ORIGIN      
        1 ttgccctcac cccaaccctc tcccggaggg agagggggac tttttgagac aggctcttaa
       61 tagccgacgt cctcccccta aaaccgcggg caagccgagc gaatgtgacc ctgagaagca
      121 agcggacgtg attgctacag cgcgggaagc gaccgattca acggaggttg aaccactgct
      181 agtgcacgag gtcaacggtc tgctcgagtt gaactggcaa cctttgttcc aatcggtggc
      241 cacggcgatg tcccgcggga tgagccggga actattggcc ctagcctttc atgttcgcct
      301 ggttgactcg gtttttcagt tgtaccaaat gcttcggtgt aggtccctgg tgatctcggg
      361 cggctgcttt cagaatcaat tattgcggga attgatcggg gcagaggcca agcgctttgg
      421 cgttccgttg taccaaccgc agctcatccc ggcaggagat ggggggatcg ctttgggaca
      481 actatggtta acggcccgaa cgcttcaggg cttggtccgt gccgattcat tgtgcgagta
      541 tattcgacct taagataact taaagaaagt gagacgaacc catgtgtttg ggcattccag
      601 gaaggatcct tgataaatac gaacggaacg gtctccttta tgggcgtgtc gattttggcg
      661 gaatcgccaa agagatctgc ctcgcgtacg tcccggaagc caaaccggga gagtacgtca
      721 ttgtccatgt cggattcgcc attagtcggc ttgatgaatc cgaggcaatg gaacaactgg
      781 ccctcctcag ggagatcgac gccgccggcg aggctgccga ggaaccgcag gatatggaga
      841 aaaaggcgtg aggttcattt ccgagtatcg tgacagcaca gccgtccgtc attggctgcg
      901 agagcttcaa aatctggtga cgcggccctg gacgattatg gaaatttgcg gggggcagac
      961 gcattccttc ttgcgcttcg gcctggacga acttttgccg aaagaaatca cacttgttca
     1021 tgggccaggg tgtcctgtgt gcgtgacgcc agtggaattg atcgacaagg ccgtggccat
     1081 tgcatcgcga ccagaggtca ttttctgctc ctttggcgac atgttacgcg ttcccggcac
     1141 aaaagacgat ctcctccggg tcaaatcacg ggggggtgat gtccggattg tgtattcgcc
     1201 gcttgatgcg atcgacctgg ccaaacaaca ccccgaccgc caagtcgtct ttttcgccgt
     1261 ggggtttgag acgaccgcgc cggccaatgc gatggccgtc ctccaggctg ccgaggccgg
     1321 tctgaaaaac ttttccgtgt tggtggcgca cgtcagggtt cccccggcaa ttgaggcaat
     1381 cctctcctcg ccgcggaatc acgtggaggg tttcctggcg gcgggacacg tctgcacggt
     1441 gatgggaacc gaggaatatc tgcccctggc agatcgctac cacacgccca tcgtcgtcac
     1501 aggatttgaa ccagccgacc tacttcaggg cttggccatg tgcgtggcac aactggaggc
     1561 cggcaaggcc gaggtggaaa tccagtactc acgttccgtg cgtccggaag gcaatattgt
     1621 ggcccgtcga aacatcgacc aagtctttag gatcgtcgat cgcaaatggc gcggcatcgg
     1681 tatgatccca caaagtggcc tcgcactgag ggatgaattc ctcgacttcg acgcggagca
     1741 acgttttgcc acggcagatg tgacggccga agagtcgccg gagtgcatag cgggcgaggt
     1801 ccttcaaggg cacaagaaac cgatggcctg cccggcattt ggtacgcgct gtactccagc
     1861 acatcccctg ggagccccga tggtttcgtc cgaaggcgcg tgtgcggcat attaccgcta
     1921 ccgccgccac gaagccggct gaatgaggcc gcggccataa ccggctgtgg acaagaaaaa
     1981 ggaaattcct tggttgagtg ttcgaacgat cgcaagacgg tgaattgaca ttcacagtac
     2041 catcggttac cataccgttg tttacagcag gcggcaccgg cccccgcggg cagcactgac
     2101 gtgctggagt cctgggcgca gaggcggaat agattggctg gttcaggata aggtttggat
     2161 tctcgagcaa tatagtgggg ctttcgtttt ggccgccgtt ctgtccggct tactgagaac
     2221 cgtccagtac agccaggtcc tctttgacaa gtttcaacgc caattcaaga tggaattcgg
     2281 gggcgatttg cccgatggag ggagtcgctt ttggctagca aggatctgtt agccagggta
     2341 cgttgtgtat tgctggtgcc aaaaatgcat ctcaaaagca actccatcac tctacttttc
     2401 tggcgttttg attcgctgaa aatcctcgct ttgtacggtg caggagtcga gcacataagt
     2461 tatccaaagc ggtggtatgc ttaaagacca aactaaaaag gggggcctat ggacaaggta
     2521 aagatcgctg ctgaattcga tccgacggcg gattttgttc tatacgaggg taattgcctg
     2581 gatttgcttc ctcgaatccc tgatggattt gtcaagctcg tagtcacttc accaccatac
     2641 aatctgggca agccttacga aactcgcctg gacatggatg cgtatctgag ccagcagcgg
     2701 agggttattg aagaatgcgt gcgagtttta gacgaccaag gaagtatttg ctggcaggtg
     2761 ggtaactatg tcgataacgg cgaaattatc cctcttgaca tcgttctgta tccgatcttt
     2821 gcctcgttag ggttgcattt gcgcaatcgt attgtgtggc actttggaca tggcttacac
     2881 gcttcgaaac ggttttcggg ccgatacgaa gtccttctgt ggttcaccaa aggcgatgag
     2941 tacaccttca agctggacgc ggtgcgcgtg ccgcagaagt atcccaacaa gaaatacttc
     3001 aagggaccga agaagggtga acttagtggc aaccctttgg gtaaaaatcc gggggacgtt
     3061 tgggaaatcc ccaacgtcaa agcaaaccac gtggaaaaaa ccatccatcc ttgccagttt
     3121 ccggtcgaat tgatcgaacg tctggtgctg gcgatgaccg acaagggtga ctgggtgttg
     3181 gatccgttca taggtgtggg aacaacagca atcgctgccc tgatgcatgg ccgaaaagcc
     3241 attggtgccg agatcatgcc tgaatacgta gagattgcga aagagcgcat ccgcctggca
     3301 gagagagggg aattgcgcat caggccgatg gaaaggtcgg tttatgaccc cgatatgcca
     3361 gacgccagta tcccacccca agtcgtacgt ctggggtcag cacgaacgga gctcccttta
     3421 ttcgagcaga ttgcccggta ctctgcgcag gagcaagaag aatgaggatc gtgtacgagt
     3481 attcgcacct gggtggggcg gaaatcttga aagtgcatta taaagaacat gaagacgaga
     3541 ttaaggaaat cattgccggt gtgaaggcaa gcaaaactaa gatcagcaaa gagaaaacga
     3601 tgcaaggaag agaactgtac tctcctaagg atatgaatca gcagtttcgt gaggcattcg
     3661 cagccagggg ttatgctgag ctgcgcgaca catacacgat taccataccc aatagcgatg
     3721 tagccatccc cggtgcgttt aagcagattg atttcgtaaa agggaaggtg ctggtggaag
     3781 tgcagttcgg caagtatgca tttatgtttt acgatatggc taagttccag tactttttta
     3841 acgagaataa ggcggacgtg ggcgtcgaga ttgtcccctg ccacgccctg cataagcaaa
     3901 tgtccaccgg cgtatccttc ggagaacaac tggtatacga tattgagcgc ttgaagcgac
     3961 atttccctgc cgtcccggtt aaggtgattt tgattgatgc agacactaat tgagacgcct
     4021 ctcaataaac cctcaatgtt catttggccg catgcgagca catagtgggg gtgcctacca
     4081 cacgctgtcc ggttgtcctc gcagcctgca cagcctgcca ggatgctgtc cgaacaaaac
     4141 gtcacaattg tttggccgtt gctgcttttg ttttagtttc gctatccgtg catgccatga
     4201 cggaccatcc cattcggggc ttctacgcgc tggtaacggc tttggggagg gccatcagcg
     4261 ccattgtcgc cggacaaata gttgaaccaa cataaggctt gcggagaatg cttatggtct
     4321 cgacaattgt tggtaccact ctttgagcct ggacaattct cggcaaatcg gttggttgcg
     4381 aggttgacaa gccagatacc cctattcgag accatggatt ttcaacccct gcggccatag
     4441 tgctcctcgc gggatcacaa gagtaagaac cccgtaaaga atcacgtgct ttggaaacgg
     4501 ttactttcgg gaggcctgaa taatgattca agacaagcgg agtaatattg gcatcgttgg
     4561 tgtggcaatg ctggtgctct tgttgccatt gggcggactt gctgaaacga tcccctcgcc
     4621 ccttcccgat cccgacggca agcctgcaga catgtcccaa cctgtccagg tcttcatact
     4681 cttgggacaa tccaatatga tcggattcgg caaggtaagt gggggcgaag gctcgcttga
     4741 atacgcagtg aaagaaaaag ggctttatcc ttatcttatt gacgacgccg gcaattggac
     4801 cgtgcgttac gacgtccgct atgtccgcgt gatgggcagt ggcacaggcc ccataaaggt
     4861 cttcaacaac gagtggttga ccgtatcggg gcgaacgatc ggcccggaaa tcggtatcgg
     4921 acactacttg ggagaagccc ttgctgcccc tgtgctcttg ctcaaaagtt gcattggcaa
     4981 ccgctcgctg ggctgggact tgcttccgcc aggttccaag caatacgagt acaaggggaa
     5041 gatttacgct ggctataaag aatcgcctat gagttgggat aagggaacaa agcccgagcc
     5101 aatcggctgg tatgccggca tgcagtacga tggtgacatc gctcgggcaa aaatggtgct
     5161 cgaagagctt gacaagtatt accccggcgc gaagagctac gaaattgcgg gattcttttt
     5221 ctggcagggg gataaggacc gctacaacga ggctcatgcc gttcactatg aagagaacct
     5281 cgttcgcctg atcgaacagc ttcgccggga tttcaatgcc ccgaacgcca aattcgtctg
     5341 tgccacgctt ggtcagaccc agaagggcgc caccggcaac gatggattga tcctcgaggc
     5401 acagctcgca gtggacggca gaagcgggaa atatccccaa tttcagggca acgtggctac
     5461 cgtttacact tacccgcttt ctaaaggcgg cgcctccaat aaccactacg gcggtaatgc
     5521 cgagacttac atgaacgtcg gagaggcgat gggcaaggcg atggtcgaac tcttgaatga
     5581 agcgaaaaaa gatcctttac cgtaaacacg ccggcccgcc ctacgttcct tctgtaatcg
     5641 ctcacagaga acaaaggacc ttcccgaaag atacccgtaa tagcagggcc agtgccttag
     5701 ccacctaggg agggtcagcg tccctggcga cccagagagg tgacctcagg aaggtcgccg
     5761 tccgcggtga ccaaatcatc ggacggcggg gacaccgtcg ctgcctcctc ttcgcccacc
     5821 ctacaaagtc cttaccgcgc aaccccttga gtcgcaagga gatgccgata tgctctgtaa
     5881 acggttaggt tccccctacc tcgccaaaaa ccggcggagc acaatatcgt tgtggcttga
     5941 ccatgtcacc tctcacagat aagactgact ttccatctgc tcgtcacacc cattttcatc
     6001 cagagtcgcg ggcgtgtgtt gcaaaatctc cactgctgat ggtcccatca agcttgtgga
     6061 gtcagggaag ttgtaacaaa agtactgcat caccagaaaa tggtgacgtg aaatcccggg
     6121 tataaccgcc ggtgatttcc gcacgctcgg gatctgggtg ccattttccc gagcggggat
     6181 cgtaccagcg gatgcgccac tggccgggag gtaggtgcat ccgacaagtg gccggcgtct
     6241 cggacacatt ggccgtctcc ggcgtgggag agtcggggtt ttgcagataa gcgatgatcg
     6301 tcttcttacc ctccatgact ttgaccaaat gaccgttatt accggccatc gcatcgttgg
     6361 gttctaagcc gaccagtgtt agcccacttt cttcaaagaa tcgcttgatg tgaagaaaat
     6421 ccgccgcacc gtcatcgaga cggccgtccg ccacggcggt ccgatagcct tggattcccc
     6481 ggattccttt tggtccgtcg aacggttcat aggtgtttaa gccgccgtaa gtggcctggc
     6541 cgccgctaag cagattggcc cacatgagtc gccgaaagaa ataccggtcg tgttgaggtg
     6601 agatgtaaat cccgtaccgg tcttcatcca acacaacggg gcagttggtt agccgccggt
     6661 attgcagcac gattgctccg gccacttggt cccggtcttc caaggtgaca atgtccgacc
     6721 atgcagcatt cacgaaggaa taaccttgga atcgctgctg gtgatttgtc agtagtgttc
     6781 cccagggttc acgccgtttc atatcatcgc cgatttgatt aatgacagcc gggtccgcgt
     6841 tccggggacc aggacctgac gaaatgtgag cgtcgttcga gatacaccaa tacacgttgg
     6901 gaaaagccga gaagcgtgcc tgggcgtaac gggccaccaa ctgggcagcg cggtcgccct
     6961 tgccataacg tgctaattct ggccagtcct cgccgtaaat aatcacctga aactgaatgt
     7021 ggggatactt ttccaaggcg taacggagtc gccgctcgat ctcgtcccaa taggccaggt
     7081 ctaacccctg ccgatccggg gcaaagagtg ccccaatatc gtgccgccca ctactgaacc
     7141 aggtccgcac cttggtaaaa cccacctcga cggcctcgtc aatgtactgt tgccacaatg
     7201 gttctgtggc ggctacaaag cggtaggcgg tatcacccag gtggagaaac cactgaccat
     7261 tgtcgtaggc aaactgtcgt gaatcagcgg gatgcttgcg gagcttgcca gggaggttcg
     7321 acggttgaac ttcaaaactc cccgatcgac cgtccaattc cggctgattt gagacgctcc
     7381 gccacttcca tcgcccaagt tgggaacagt acgcgcgggc ggcccagcga ttctctcctt
     7441 tgaagaaacc ttccgccttg acctctgagc cgtcgggcaa ggtgaaaatg atgtggaact
     7501 ctacatcgaa gaaggggttg ccacgcacac ggtccagtgt cagctctcct tcccaaacac
     7561 ctcggtgggg agcggtaggc gtccccgaga cgaaagccgg tgccgcattt gcccatgttg
     7621 tcaggaaaag ccccaggatt gccgacatcc cccacgtcgg ggccgataat tggaagtggt
     7681 gtctcatcat gctgtgcgaa cctccttttg gtgttttcag gttcccattg tcccaagttt
     7741 acacgggaca actgtggttg actaacggtc gggaactgaa tgacccttaa agcctgaacc
     7801 ggcagagcga aaaccccgtt ccctcaaaaa attcgcatag aagtagaatt acgatggttc
     7861 accgcggcag attcaaggag acttgccctc ctgtcataaa acaatcggcc acgatggctg
     7921 tgcacgcact gtcgctggca taccaaacca agcgcttcgc tcaggcgggg aaacagtcca
     7981 ctgaccgtgc cccttattcg tccctcgccg ctaccgctgt cgaacggcgg actccggaac
     8041 aagcggatcg acgatcatcg ccgtttgcgt gccggaaaag tcgttaccaa caacggcaat
     8101 gtcgcgagtt gattcacctt cgacgcttaa aaacggagta ctaacttgct cggcgcgaca
     8161 accatgcaag aggaccccct ggcaattgac catccggact gttggcattg cgctgaccgc
     8221 agcagcacgg agctttgtca aatacgcatc atgcacatcc tcgaggacca atgccggtcg
     8281 ctcttcaccc cgggcagtgt caaagctcag ctccacgcca tcaagtgtca gatggcggac
     8341 atggcgaata aacagcccat acgctggcaa aggaccgaaa atccgaggcg acggataagc
     8401 ggcctcgcgt tcaggaacag tggcccggcc gttttcaacc gttccaccac ctgcggactg
     8461 gatacggatg tcgcgcaggg ttaggtgctc aatacaagcc ccaggaatac ccgatatggc
     8521 acagccgagc acaccggcgc cacgggcttg gattccgctg atcacaatat tccgcatcat
     8581 gccttgcccc ggcggttctt catcgggcaa aggtcgggca cgattgccga gacggataaa
     8641 gatgggcgac gcaacgttgt ccatcaccac attcgagatc gacaccctat ctagcactcc
     8701 cccgtcgacc attagcaagg agataccggc tccgcccgtg tcatagattg tgcaattcga
     8761 catgacgata tcctcgaaac ccccattcga ctctgtgccc agcttgaagg cagcagcttg
     8821 gcttgacagg tggcaattaa cgacggtcac atttttgcac gggcggtggg ccgtggcctt
     8881 caagacgatc gcgtcgtcgc ctgtttcaac ccggcaattc gccacacgca cccgatggca
     8941 actgtcgatg tccagtccgt cgcgattctc acgcttgctg gaaaggatgg tgacaccatc
     9001 gatcaaaaca tcatcgcatc ccaagaaatg ggcacaccaa cgggccgatt caagcagcgt
     9061 aacctcacgc acgcgaactc ccgtgcactc ggcaaaacga aggatgtaag ggcggtctcg
     9121 gtcgcccggg ttcgcccgcg catcaaaggc ttcattggtc ccctggccgt caattgttcc
     9181 cggtcccgca atcccaatgt tcgtttgttt ctccgcgtaa atgagttgcc gagtaaaccg
     9241 atcccggtat acatatttca actgcggcgt aaagtcggga tagtcatctt tatcgagact
     9301 ggcgcgcaac acggtgccgc tgtcgatcca caaagtgaca ccacttttca ataccaccgt
     9361 tcccgataaa taaacccgtt ttcccgttag caggacgcgt ccaccaccct cggccgcgca
     9421 atggtcgata gccgcttgga tcgcttttgt gtccagcgtg acaccatcac ccacggcacc
     9481 aaagtcacgg acatcaaaga cacttgcaag acactcgcgt gcagcaaggt ttgctgtgtt
     9541 tacgacaacg agaaggaacg cgacccacgt gcgacgaatc atagcacacc tcccgtggtg
     9601 attgggggat gctaagaaag gtttcacggt tttttcggtc caggttttag gtccgagatg
     9661 gggaaggtcc gtgctgcatc gtccaggatc agcacccaat cgttgccctc gcctggctct
     9721 cctggtgggt cgaacgaccg gcgaccgcgg gccggaaatt gccctatctc gctcgtgttg
     9781 cctgtgcggg gatcaaacca cgcagctttg acattcgcaa agccgagctt gtccagggca
     9841 atttccaggg ttcgaccggt cggtgtgtac acgagggcgt agccatcccc gcgtaacgcc
     9901 aaacacattt cccaaggctt cgtctgttca aaggcgagca aggaaaggtc aggccgttgt
     9961 gtgagaaatg gtcgggactc gatcaaccgc cgcaagtatc ccatttggaa tgcgcttggg
    10021 gcatcgaggg cttcgtacca atactccttg ggtccggcga ccccaggata tttgggcgag
    10081 tgcatttgcc aaatactgtg atggccgtac gtgtgcccgg ccgcgcccgc gaaaaccgcc
    10141 caataagcgg cccgccgcgc gtcatcgtcg gtggccaccg tgggggggcg accatgtcga
    10201 aaacccgggt aacttgtttc caaatcaatc accggcaacg tgggtctcat ggtgtaggcg
    10261 tactcgacca tcatccaatt cagcgcccag cggccatgcc cggattgcaa cccatggatg
    10321 tcaagccacg gttctttgtt caaaaaccag gccgtgctac ctggaccact ggtgtgataa
    10381 gtcatcaaca ctttcgagta gtcttcctca cccgtcacac cgatggcgat accccgtgcc
    10441 atggcccgcc agatcgcctt ggcttcatcc gtgggcgagg ccttatcgcc ccccaaaacc
    10501 caaatgatgt tgttgtgatc gcggaatcga ttgccaataa accggccata aatttctgcg
    10561 ttctccgtcg tgaaaaaccc atcgacgatg ccatcctgcc aattggaaat gacccattta
    10621 ccccacgttg gcagcagagc gacatacagg ttgttctcct ctgccagccg caggacgcgc
    10681 tctacgtcat cccagtaatc gttgttaggc cctggttcga caatcggtcg atccggtcgt
    10741 ctcggttcaa taggaaaatg cccaaaagcg ttgggcttat cgctccgcaa ctctacgagc
    10801 accacggttt ggatcaccgt aaaacccttt tcccggcggt cggcgaagta gcgccgcatt
    10861 tcggcctcat ccaagtcgtg gatcagttgc caagccgtat ccgccaccca aaagaaaggt
    10921 ttgccgctgg cagtcaccag caaccgttgg ttatcgccac tcgcgacggt tctaacccag
    10981 tcgccctggg caacgcgaag cacaaaacgc caatagcgtc agctaataac cacccaagcg
    11041 cgcactccat gtttatgctc ttctcccaga aaaggcttac cgaatcctgg gaaccacacc
    11101 accaggtgca accatcattt ctcgaggacc acttcagcgt tgatgctcac ggatcacttc
    11161 taaagggagc gccccccggc cactaacacg gctgccgaag attttcagac cacgaatttc
    11221 tccgcggaac tgtcggtcca gaccgtaacc gtgcatcgtc ttgttgaagt tgccgatggc
    11281 cagcggaccg atgtccatgt caaccggtcc aacgttataa gaggtgatac ggtccaggac
    11341 aattgcggcc tggccaggtg actctcttgg ctcgctgaaa taccaccgca cattatcccg
    11401 cggctgagtt gcgtcatacg ttaccgcgaa aaacgtccat cgcccgagtt gcaacttgcc
    11461 gggctaacta tcgttttgga cgctgtcggg ccatttgttc accgccaagc ggagacgacc
    11521 atccgcgtgg caaaccaggt cgatccccga ccgattctcc ttcagacaga agacaatccg
    11581 attgccgccg ctacccactt gcaggctctc cggcttgacc cagccgagaa tcgtaaacga
    11641 ccgtaaaccg cggagtccct cgacaacgtc atcaccgacc tcaatccacc cccacggagg
    11701 gcccggaggg agaaggagcg acccgtcggc cccacgtttt gccgtgccct gaaacgtcaa
    11761 cgtcggtacc tctccacctg caaaacccac acgaacaatg gggacgttgg gatcgtgatc
    11821 gaccaggatt tcctgaaagg ccgtcgcgct ttgaccctcc gccgtggtgg cagttaaggc
    11881 gacgcagtac acgcccggcg attgaaaaac atgccgcacc tgtgggccct cgccggtcgt
    11941 tccatcaccg aaatcccaac gcagcttgcc tacggtggga ctgctaaact ctacttccaa
    12001 cggggcagga ccttcggtgg ccgaggcctc aatccgcaca gtaggccgta tgggctcgcc
    12061 aggttgatag gccacgaagc gaaaggccac atcagcacgg ggtgggagca ctgcgaattc
    12121 ccccggttcg gctgtcccca gggccgtaat ggtcatctcc caggggtcga tgaggtccac
    12181 tttgtaacga tgtccctcgg ccaggttgat aacttgtttt cgctgatcaa cacaatagac
    12241 caagtagaac tcgcccggct tggccaacaa gaaacggcct cggtcatctc ccatgggctg
    12301 aagttcgtca aaggcgggac actgggtaag gaactgctta agccaggcga tgcgggccgg
    12361 gctttggccg tgcaacacgc cccccttcga ccaccacagg atatcctgcg gatgcttgta
    12421 ggtttccccg tggcccacat agcaaccgct cagtgttccc agccaaaacc gctgggccat
    12481 ttcccgtggg gtaaggttcc cccatccctg cggaatgtca ccttcgtaac ggcattcgtc
    12541 gtagatcacc ggcttgccaa attgcgtacg aaatcgcacc ccgccattca tgtcggaact
    12601 ttgcaaactg gcgtgggtaa cccagggctt ggtgtgatcg taaaacaccc ggcaattatg
    12661 gatgctccgt aatcgctgat gggggtcttc cttctgaaga atctgaaaga aacggtccca
    12721 gtcggccatg gtcttatcac ctcggtggcc ccgcgctgcc cctggggcca tcaagtcgta
    12781 ctcgttggcc agcgaccacc agacgttgcg aaaggcactt agccgtgcaa tgcaatagcg
    12841 gagataacga tcatcgtttt cgcggcccat ctcggcgaaa ccccaacggt cgtaggggtg
    12901 ccaaaggatt aaatcggcct cgatgcccaa ccgttgcagg tcgagaatgc gtcgctccag
    12961 gtgccgccag aa
//
LOCUS       DAOSUT010000120        12950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2856_length_12950_cov_9.638154, whole genome shotgun sequence.
ACCESSION   DAOSUT010000120 DAOSUT010000000
VERSION     DAOSUT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12950
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_12950_cov_9.638154"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLD05_11220"
     CDS             complement(<1..573)
                     /locus_tag="PLD05_11220"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOP78052.1"
                     /translation="MRRDELTELHYIAHMDNVPSILRRGILSYRRASEIPHTSVAMQV
                     VQNRRAEVEVPGGRMLHDYVNLYICARNPMLYKLQGQHLKLCVLQVSTDVLDLPGVVI
                     TDRNASSAYVRFAAAPNGLKIVDRDLTFAEDWQDADQIKYYRKKSAKCAEVLVPDEVP
                     PEYILGAYVSCQQAMDSLNGLGTGIRVTING"
     gene            complement(586..4116)
                     /locus_tag="PLD05_11225"
     CDS             complement(586..4116)
                     /locus_tag="PLD05_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOP78053.1"
                     /translation="MAESRFSTQIGRQISLPGHFDVPVILEDVRALGGDGSGGYECRV
                     RLPDGTLEEAVISPAEAATLFSTDRESPTAITPADAEQLRLLIESARIRLAYAHDRQF
                     AVSLSGIRTLPHQIEAVYQAMLPQPRLRFLLADDPGAGKTIMAGLLIKELKLREAIER
                     ILILCPAPLTIQWQDEMLRWFGESFDIIFSAVDQQQLTNPWQRCSQVISSIDYAKQND
                     VRERVWQQRWDLVVIDEAHKCSAYTKSRTNRSDEVAETKRYQLAKRLSETADHVLLLT
                     ATPHHGDEDKFSHFLRLIDPDLFPEPHRLGNQATSIRKDVFKLGKDSPWCLRRLKEDL
                     RDLNGKRLFPDRHARTVTFRLNSEEYALYKSVTAYINEFIPGAQASSAGGQRRSAAAL
                     TRTVLQRRLVSSTCAIHESLKRRLKKQQDLLDELEGLSPAQRAKRLAALQKRLPDAEQ
                     EEDDLDDEVRDRLVDEYTAALELEQLRAEISALKELVEQARRVQDLERDSKLAALKKC
                     LQEAQFAELRDGRGKLLIFTEHRDTLTYVREHLERWGFTTCEIHGGMNPHERKRAQEQ
                     FRTTAQVCVVTEAAGEGINLQFCHLMINYDMPWNPTRLEQRLGRIHRIGQDRDVYAFN
                     FVATESEDGQPIVEGRILHRLLEKLDTMKEALKGRVFDVIGEVLSLNDVNLPDMLREA
                     AYDPRRLDEYLDQIDRIDPEKLKEYEEATGIALARSHVDFTGFQRRNLEVEEKRLMPR
                     YVEAQFIAAAKEIGLRVEPRADGLWRIEHVLAELRTERLRSVQRLGKPDPSYRKVTFH
                     KDHLEQSAHVDAVLLGPGHPLYAAVDEKLNEKLAPLQGKIGFYVDPMATEPYRLHFFE
                     ISIRGKDSKGNDVPLHGELVAVREQGGQFEIVPSHILLNLPAHPRPPALTNPVAVQAA
                     SDYLKSTYQLECRARCQKERQHFAGICRDYLERSFAARIKRAQERAMELMAAASTNPD
                     FKLAADEASKYVEELQRQRAERLSGLGRLEIARTGPVRHLATAIVLAAGPDTEAQLAD
                     LADELDTDLRRQSELAAENMVVAALKEEGFPEDRIERVGHLKLGFDVRAHRIADPTTG
                     AIVVKRIEVKGRRRGQAVRLTTNEWYMAQQLGETYWLYVVWDPLGPKPELVRIQNPAV
                     RLDHAKREIVAARFFEIPADAVVDAAKVQG"
     gene            complement(4698..4883)
                     /locus_tag="PLD05_11230"
     CDS             complement(4698..4883)
                     /locus_tag="PLD05_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78054.1"
                     /translation="MGSGAVESMDKSVAGGRCTQAGLRNWTRRGAEAVLWLRAVQFDD
                     FGLLRDAQLRLALINPP"
     assembly_gap    5134..5233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5901..>8387)
                     /locus_tag="PLD05_11235"
     CDS             complement(5901..>8387)
                     /locus_tag="PLD05_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP78055.1"
                     /translation="HTCAAGDGKVFFGAADGRIYAVRAADGELVWSVPTNAAVWNAPL
                     FWKGLVVIGSRDGKLYAVEADLGKLRWTAQTGGPILASPALDAKSQRVYVASEDMHVY
                     AIELTDGQIAWRSEKLPGATFRGYHPVVAPDGAVMVTVAPVMSQDSINPILWDMVREI
                     FGDIASWRHTKEENERLREQNFKQLAQPDTYQRQLDYLRKRLAEQPAYQTFFVLDPKD
                     GRQRFVAPIVYAESMNGTGAPAIVAPDGRVIVKYQVLLRSRYEHYSPFLNVGYLDTKA
                     GHITPIMDQSRTYGWYDSLLLVHDEQCQLVVAGRVLINTHQDNVNAMDLETLQGYTAP
                     FCTNIHEPKPGEALGIWACLIRGQPLPPGKEWLIRGTAVYGGGSVIDVPVSVAGDSFY
                     YIPAHELNAGCAVIAYRMQRPGESGHPVPADLPRIGSEEEEKVRAMPWDWDVLGTPRL
                     RKAIDELRPPVPGTWSAPLTEATTAVAKIPDAELDEIIWEVPAIPSTSSSRATSLRAQ
                     LSAAVTELISRDWRPLVFPAGKHPIEAYRFFAEPTETLLTLARAYPHLDPGLQKRVAE
                     KVAAMAAPGGPLDGPTGTRTFDPNVGEVRSAYSVPPENLWRVTDDILRTSLARLYPFW
                     LWAYMTGDWSHLEKHWGSIRDLVAQSPNDPKEDCCNGHIAGLIAYCRIARHLGDNEAV
                     RLGVNAARQAMRDRVAFEWAHPSGAVLSEVPVQRQVFARWRNLTPEVARLCSEYAGDL
                     QRQLIERYVDYHRPTWWLAWNVELAWRNECPFSLPTMSLEIFAAKAMILGEPAERLCQ
                     YLDIPWCKADLYYIQKLVWCIEAEAGTTSQ"
     assembly_gap    8388..8487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8488..10202)
                     /locus_tag="PLD05_11240"
     CDS             complement(<8488..10202)
                     /locus_tag="PLD05_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOP78056.1"
                     /translation="MWLTAASVLVCTLAISPNAKFEPPQLGAARWTSGFWASRFETCW
                     HQTLDALERAMRDPANGAQLSNFEIVGQPGAEHRGNNWSDGDVYKWIEAMARVYAVTR
                     DEALDRKMDEWIEKIARTQADDGYISTQTQFNRHKERWGMRQYHELYNMGHLITAAVI
                     HYQATGKDSFLKVGRKCADYLYTTFQPRPPQLAHFGWNPSQIMGLVDLYRVTGDRRYL
                     ELSEIFINMRGSARWPGPSAFAELAKIGDPHPGDQTQDRVPLRKEEFAVGHAVTGAYL
                     YCGAADVVAETGDKELFAAISRIWKDIVQRKMYITGGTAAYHHGLSIRLDPVHEAYGA
                     AYDLPRRTAYNETCANIAHAMFSYRMLALTGEAQYADIMEQTLYNSVLSGISLDGTKF
                     FYTNPLECRQDAPLLSNDSKERWSTWKCFCCPPNIARTIAGCSQWAWGFSNDGVWIHL
                     FGTGEAQGKCAGDEVHLRQESNYPWEGKITITFVKVPNRSVTLRVRIPAWADGATCQV
                     NDEMPQLCTAGTYHTLARTWKEGDVVRVELPLSVRLLESHPWVEQNRNLVAVMRGPIV
                     YCLESV"
     gene            10888..12918
                     /locus_tag="PLD05_11245"
     CDS             10888..12918
                     /locus_tag="PLD05_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOP78057.1"
                     /translation="MKSKEHVHRLCDEKWHSLEIADIYERLQSGPDGLDQQEAQHRLA
                     YYGPNTLPAKQPPTIWAILLHQILNPLIFILLGAAVALLAIGKGADAIFVLIVIALNS
                     TLGAYQEYHAEKSAAGLRRLLTITARVRRKGKEIEVPAEEVVPGDIILLESGNKVPAD
                     MRLVEVNNLAADESFLTGESVASEKTTHILPEDTVVGDRKNMAFAGSTITSGRGVGIV
                     VATGSETQVGLIAKTVSESEMGKPPLVLRMERFVKQITILVLVISTFLAVLLWFQGND
                     IPSIFFFVVALAVSAIPEGLPVALTVALSVATRRMSARNVIVRKLTAVESLGSCTVIA
                     SDKTGTLTVNQQTVRVVDLADGRTFEFTGEGYSGEGQVFRKGGEITPTDHEQLTRLAE
                     AAILANEGSLVREEGGWKHRGDAMDVALLAMGYKLGLSPTEARRQVKILFEIPYESEH
                     KYSAVFYEKNGTTYVAAKGAVETILDFCSGVLRDGTPLPLDRDDIERRAQAMAEKGLR
                     VLAVAGGQTESLPSEASDQAKFLSGLLFYGLVGFIDPLRPEAIEAVRTCKSAGIKVLM
                     ITGDHPATAGAIARELGLSDIDEPVVTGAQLNEAGPTESPAFEKLVTSSHVFARISPT
                     QKLEIVDVLMREGELVAVTGDGVNDAPALRRANIGVAMGSGTDVAKLDFCQS"
BASE COUNT         2554 a         3787 c         3710 g         2699 t
ORIGIN      
        1 cccgttgata gtcacgcgga tacccgtccc cagcccgttc aagctgtcca tggcctgctg
       61 gcacgagacg taggcgccca agatatactc cggtgggacc tcgtcgggaa ccagaacctc
      121 ggcgcacttc gccgatttct tgcggtagta cttgatttga tccgcgtctt gccaatcctc
      181 tgcgaaagtc agatcgcggt ccacgatttt caacccattc ggcgctgcag cgaaccgaac
      241 gtacgcgctc gacgcgtttc tgtccgtgat gaccacgcca gggagatcca gcacgtccgt
      301 gcttacctgg agcacgcata gtttgagatg ttggccttgg agcttgtaga gcatcggatt
      361 tctggcgcat atgtaaaggt tcacgtagtc gtgcagcatc cgtccccccg gcacctcgac
      421 ctctgctctt cggttctgaa cgacctgcat ggcaaccgac gtatggggaa tctccgaagc
      481 cctcctgtac gataggatgc cccggcgaag gatcgacggc acgttgtcca tgtgcgcgat
      541 atagtgcaat tccgtcaatt cgtctcgcct cacggatacc catcctcatc cttgtacctt
      601 cgctgcatcg acaaccgcat ccgctggaat ctcaaagaac cgtgccgcga cgatctcgcg
      661 cttcgcgtgg tccagccgca ccgcagggtt ctggattcgg acaagctccg gcttcgggcc
      721 gagcgggtcc cacacgacgt acaaccagta ggtctcccct agctgctggg ccatgtacca
      781 ctcgttggtc gtcaggcgga cggcctgccc gcgcctccgg cccttgacct caatgcgctt
      841 cacgacgatt gcgcccgtcg tagggtcagc gatccggtgc gcgcgcacgt cgaagccgag
      901 cttgaggtgc ccaacgcgtt cgatgcggtc ctcggggaag ccttcctcct tgagcgccgc
      961 aacgaccatg ttttcggcag cgagttcgct ctgccggcgg aggtcggtat cgagctcgtc
     1021 cgcgaggtcc gcaagctggg cttcggtgtc agggccagcc gccaagacga tcgcggtggc
     1081 cagatgacgc acggggccag tgcgcgcgat ttccaagcga ccgagcccgc tgagccgctc
     1141 cgcacgctgg cgttgtaatt cctccacgta cttgctggcc tcgtcggcgg cgagcttgaa
     1201 gtccggatta gtcgaggctg ccgccatgag ctccatagcc cgctcctggg cccgtttgat
     1261 ccgggcggcg aaggatcgct ccaggtagtc ccggcagatg ccggcgaaat gctgccgctc
     1321 cttttggcag cgggcgcggc attccaactg gtaggtgctc ttgagataat cggacgctgc
     1381 ctgaaccgcc actggattgg tgagggctgg gggccgcggg tgggcgggga ggttaagcag
     1441 gatgtggctc ggcacgatct cgaactgccc gccctgctcc cgaaccgcca cgagttcccc
     1501 gtgcagcggc acgtcattgc ccttggagtc cttgccacga atcgaaatct cgaagaagtg
     1561 gagccggtat ggctcggtcg ccatcgggtc tacgtagaac ccgatttttc cctggagggg
     1621 cgccagcttc tcgttgagct tctcgtcgac cgccgcgtag aggggatggc ctgggcccag
     1681 gagaacggcg tccacgtgcg cagactgctc gaggtggtct ttgtggaacg tgaccttgcg
     1741 gtacgagggg tcgggctttc ccaggcgctg gaccgaccgc aggcgttctg tccgtagctc
     1801 ggccaggacg tgctcgatgc gccataggcc gtctgcccgc ggctccacgc gaagaccaat
     1861 ctccttggcc gccgcgatga actgcgcctc cacgtaacgg ggcatcagcc gcttttcctc
     1921 gacctccagg ttgcggcgct gaaagcccgt gaagtccacg tggcttcgag ccagcgcgat
     1981 acccgtggcc tcttcgtatt ccttcagctt ctccgggtcg atgcggtcga tctggtccag
     2041 gtactcgtcc agccgtcggg gatcgtaagc cgcttctcgc agcatgtcgg gaaggttgac
     2101 gtcgttaagc gaaagcacct cgccgataac gtcgaacaca cgtcctttga gggcctcttt
     2161 catcgtgtcc agcttctcaa ggagtcgatg gaggatgcgt ccctcgacga tgggctgccc
     2221 gtcctcggac tccgtggcca caaagttgaa ggcgtagaca tccctgtcct gcccgatgcg
     2281 gtggatgcgg ccaaggcgct gctccaggcg cgtcgggttc cacggcatgt cgtaattgat
     2341 catgaggtgg cagaactgga ggttgattcc ttcgccagct gcttcggtca ccacacagac
     2401 ctgcgcggtg gtgcggaact gttcctgtgc ccgcttgcgc tcgtgggggt tcatgccgcc
     2461 atggatctcg caggtagtga atccccagcg ctcgaggtgt tcccgcacgt aggtgagggt
     2521 gtcgcggtgc tccgtaaaga tcagtaattt gccgcggccg tccctgagtt cggcaaactg
     2581 cgcctcttgg aggcactttt tcagcgcggc caacttggaa tccctctcta gatcctgtac
     2641 cctgcgggcc tgctcgacca gctccttgag ggcggagatc tccgcgcgga gttgctccag
     2701 ctcgagggct gctgtgtact cgtcgaccag ccgatcgcgg acctcgtcgt cgaggtcatc
     2761 ctcttcttgc tctgcatcgg gtagccgttt ctgaagggcg gcgagacgct tggcccgctg
     2821 ggccggcgag agcccctcca actcgtcaag caggtcctgc tgcttcttga ggcgtcgctt
     2881 cagcgactca tggatggcgc aggtcgaact caccaggcgt cgctggagga cggtgcgggt
     2941 gagcgcggca gccgaccgtc gttgtcctcc agccgaggac gcttgggctc ccggaatgaa
     3001 ctcgttgatg tacgccgtga ccgacttgta gagagcgtac tcctcgctgt tgaggcggaa
     3061 ggtcacggtc ctggcgtgcc ggtcgggaaa caagcgcttg ccgttgagat ccctgagatc
     3121 ctctttcagg cggcgcaggc accacggcga gtccttgccg agcttgaaca cgtccttgcg
     3181 aatcgacgtg gcttgattcc cgagccgatg cggctccggg aacaggtcgg gatcaatgag
     3241 ccgcaggaag tgcgaaaact tgtcctcgtc cccgtggtgg ggcgttgccg tcagcaaaag
     3301 cacatggtcc gccgtctcgg agaggcgttt ggccagttga tagcgcttgg tctcggcaac
     3361 ctcgtcgctc cggttggtac gcgacttcgt gtaagccgag catttatggg cctcatcaat
     3421 gacgaccagg tcccagcgct gttgccacac gcgctcgcgg acgtcgttct gcttcgcgta
     3481 gtcgatggaa gagataacct gcgagcaccg ctgccacggg ttcgtgagct gctgctggtc
     3541 aacggcggag aagatgatgt cgaaggactc cccgaaccag cggagcattt cgtcttgcca
     3601 ctggatcgtg agcggcgcag ggcagaggat cagaatcctc tcgatggcct cccgaagttt
     3661 gagttccttg atgagcaacc cggccatgat agtcttgccc gccccggggt cgtcggccag
     3721 gagaaagcgc aggcgaggct gcggcagcat ggcctggtac acggcctcga tctggtgagg
     3781 cagcgtgcgg atgccggaaa ggctcacggc aaactgacgg tcgtgggcat aggcgaggcg
     3841 aatccgggcg gactcgatca acagtcggag ttgttccgca tcagctggcg tgatcgctgt
     3901 cggcgattcc cggtccgtgc tgaagagcgt ggccgcttcc gcaggggaga tgactgcctc
     3961 ctcgagggtg ccgtcgggca accggacccg gcactcgtag ccgccggagc cgtccccacc
     4021 gagcgcccgt acgtcctcga ggatgacagg aacatcgaag tgccccggca aggagatctg
     4081 ccgtccgatc tgcgttgaaa agcggctctc agccatgcct gctcttcttc ttcacgttcg
     4141 tctttcggcc tgctggcctc gattccgtcg tcttgcccga cggggcggac gcgaaatcac
     4201 cgccgctgct ggacgttacc cactcgtcca cctccgagag tctgaaccga aggaggcgtc
     4261 ccacacggtg gggcgggaag ccctttgaaa ccacccagcg gtagatggac tgcttagcca
     4321 cctttggatg ggcgcccact tcctcgattc cgacccaacc tacttcagga gtcatattcc
     4381 atcctacctc gcgcttcgaa agagcgtcaa tttctgacca cctggagttc acccagtgtt
     4441 gaccttaaca tggcgcggaa aatggcgaag acagctatca acccctgcgg gacgccttcc
     4501 tgagccatcc ctcgtgcttg cccaaaccgg tcaccaacct gttcgactac ttggctaccc
     4561 aaagcagcct atttctccac ccaaaaccca aaatcctcgc cctcaactat ttcgacactg
     4621 ggtaaactcc agctgaccac gacgtttcaa gagagtctac aagtcgcagg tcgcactgtc
     4681 aaacgtgtag ctgcagatta tggcggattt atgagagcca ggcgaagctg ggcatcccgc
     4741 aagaggccga agtcgtcgaa ctggacggcc cgaagccaca gcaccgcttc cgctccacgg
     4801 cgggtccaat tccgcaaccc ggcttgtgtg caccgtccgc ccgccacgct cttgtccatc
     4861 gactccaccg ctccgctgcc caccggtagg ccctcccggc gacatcgcgg gtagtccgtt
     4921 ggctaggcgt tgttcttcac agacacgggg cgctgttctt cacatgcacg gggcggtgtt
     4981 cttcacagac acgcccagcg ctcgccatgc ttcacgccgc gccgctaggg gcgtcgttcc
     5041 gccaccgcct gacgggccac aaagctctat ttcagagggt ctccaacccg agaacctaca
     5101 gagggtcttc aacccgagaa cctatagagg gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnccaaccc gagaacctac agagggtccc caacccgaga acctagatca
     5281 gatcttcttg ctcttcggat ttctcgtgtg gggttccgaa tcgccggaca gccatttccc
     5341 tcatcctcgt cgcagctgtt cccgtcccag ttgcagttgt tgccgtgcaa aacactgcgt
     5401 tgtcttgtgc gttgcgccga tttcatgtgt gacgtaagta gtgtttctga cgagacttaa
     5461 tacaaatgat agcaggattt ctaatccgct ggttgggggt tcgaatccct ccgggcgtgc
     5521 tactaagaat ccatctcaaa aagtccccct ctccctccgg gagagggttg gggtgagggc
     5581 gagcgaacgt cgcaatcaag caatcaaatt gagaaaaccg gcagcgattc gagccaccct
     5641 cccgctcgat gtctctcccg ccgaacggga gggggggaga ttatgggatg ggttccaagt
     5701 accttacgct gacttgcaaa atgggtatga cccgcaagaa acgaaggtgc tcctcttctc
     5761 gttagtgatc ttgtccgtct tgcggagagg gtttgccgac gcagtctccc cacggaagac
     5821 aatctgagga gcagcgtgct atccccggcc catcgaaatg cgacttgcac acggtggcta
     5881 atgcgtggcc tgaatccagc ttactggctt gtcgtgccgg cctcggcctc gatgcaccaa
     5941 accagctttt ggatgtagta aaggtcagcc ttgcaccaag gaatgtccag gtactggcaa
     6001 agccgctccg ccggttcacc aaggatcatg gcctttgccg cgaagatttc tagcgacatc
     6061 gttgggagcg aaaacgggca ttcgttccgc caagccaact ctacattcca ggccagccac
     6121 cacgtcggtc ggtggtaatc tacatatcgt tcgatcaact gccgctgtaa atcgccagcg
     6181 tattcggagc agagccgcgc gacttctggc gtaaggtttc gccagcgggc aaagacctgc
     6241 cgctgcacgg ggacctccga caagacagca ccgctcggat gggcccactc gaaggccacg
     6301 cggtcacgca tggcttggcg ggccgcgttc acaccaagac gaaccgcctc gttatcaccg
     6361 agatgacggg caatgcgaca ataggcgatc aatcccgcga tgtgcccatt gcaacaatcc
     6421 tctttaggat cgttggggga ctgggccacc aggtctctga tagaacccca atgtttttcc
     6481 agatgggacc agtcgccagt catgtaagcc cacagccaaa aaggatagag cctcgccagg
     6541 gacgtgcgga ggatgtcatc ggtcacccgc cagaggttct caggcgggac ggagtaggcc
     6601 gagcggactt cgccgacatt ggggtcaaac gtccgcgttc ccgtggggcc atccagagga
     6661 ccaccggggg ccgccatggc cgctaccttt tccgcgacgc gcttctgcaa tcccgggtcc
     6721 agatgcggat aagcgcgggc cagcgtcaaa agggtttcgg taggttcggc aaagaagcgg
     6781 taagcctcaa tcggatgctt accggctggg aagactaacg ggcgccaatc tcggctgatg
     6841 agttctgtaa ctgcggccga aagctgcgcc cggagagatg tcgcccgcga tgatgacgtg
     6901 cttgggatgg ccgggacttc ccagatgatc tcatcgagct ctgcatcggg aatctttgcc
     6961 acagcggtag tggcctctgt aagcggcgct gaccacgtcc ccggcaccgg gggccttagc
     7021 tcatcgatcg ccttgcgcaa tcgtggtgtc cccagtacgt cccagtccca gggcattgcg
     7081 cggacttttt cttcctcttc ggagccgatc ctgggcaagt cggctggcac ggggtgacca
     7141 ctctctcctg gccgctgcat tcgataagct atcaccgcgc agccagcgtt cagctcatgc
     7201 gcgggaatgt aatagaagct atcaccagcc acggagacgg gcacgtcgat gacggagccg
     7261 ccaccataga ccgccgtgcc gcggataagc cactccttac ccggcgggag tggttggccg
     7321 cggatcaaac aagcccagat gcccagggct tcgcccggct ttggctcgtg gatattcgtg
     7381 cagaacggcg cggtgtaccc ctgaagcgtc tcaaggtcca ttgcattcac gttatcctgg
     7441 tgggtgttga tcaggactcg tccagccacg accaattggc attgctcgtc gtggaccaac
     7501 agcagactgt cgtaccaccc gtaggtccgt gattgatcca taatcggggt gatatgccca
     7561 gccttcgtgt ccaggtatcc cacgttgaga aaaggcgaat agtgctcata tcggcttcgt
     7621 agcaggacct gatatttgac gatcacccga ccatctggcg caacgatggc cggggcaccc
     7681 gtgccgttca tgctctcggc gtaaacaatg ggggccacaa accgctgtcg gccatctttc
     7741 gggtcgagaa caaagaaggt ctgataggcg ggctgctcgg ccaaacgctt gcggagataa
     7801 tccagttgcc gctgataggt gtccggctgc gccaattgtt taaagttttg ttcacgaagc
     7861 cgctcattct cttccttggt gtgccgccaa ctggcaatgt ccccgaaaat ctctctcacc
     7921 atgtcccaca ggatggggtt gattgagtcc tgcgacataa ctggcgccac cgtcaccatg
     7981 accgcaccat ccggtgccac aacagggtga tacccccgga atgtggcacc gggcagtttc
     8041 tcgctacgcc acgcgatctg cccatcggtc aactcgatgg catacacgtg catgtcctca
     8101 ctggccacgt acacgcgctg cgacttagca tccaaggcgg ggctagcgag gatcggccca
     8161 cccgtttgcg cggtccaccg gagcttgccc aagtcagcct ccacagcgta aagttttccg
     8221 tcacgactcc caatgacgac aaggcccttc caaaagagcg gggcattcca aacagcggcg
     8281 ttggtcggta ctgaccaaac aagctctccg tcggccgcgc gaactgcgta gatacgccca
     8341 tcagcggctc caaagaacac cttgccatcc ccagctgcgc aggtatgnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnacg gattccaagc aataaacgat ggggccgcgc
     8521 atcaccgcca caagattgcg gttttgttcc acccagggat gagattctaa cagacgaact
     8581 gacaacggta attcaacacg cacgacgtcg ccttccttcc acgtcctggc tagcgtatgg
     8641 tacgtgccgg cggtacacag ttgcggcatc tcgtcattga cctggcatgt tgccccatcc
     8701 gcccaagcgg ggatacggac gcgaagagtc acggagcgat tgggtacttt cacaaaggtg
     8761 atcgtaatct ttccttccca cggatagttc gactcttgtc tgaggtgtac ctcatccccg
     8821 gcacacttcc cttgtgcttc tcccgttccg aaaaggtgaa tccacacgcc gtcattggaa
     8881 aaaccccatg cccattggct gcaacccgcg atcgtccggg caatattcgg gggacagcaa
     8941 aaacatttcc acgtgctcca ccgctctttg gagtcgttac tcaatagggg ggcatcctga
     9001 cggcattcta ggggattggt gtagaagaac tttgtcccat caaggctgat gcccgacagc
     9061 acactgttgt aaagcgtttg ctccatgatg tcggcatatt gcgcttcgcc agtcagcgcc
     9121 aacatgcgat agctgaacat ggcatgggcg atgttcgcac aggtctcgtt gtacgccgtc
     9181 cgccgtggca aatcgtaggc agcaccatag gcttcgtgga cggggtcaag cctgatggac
     9241 agcccatgat gataagcagc cgtaccgccg gtgatgtaca tttttcgctg cacaatgtct
     9301 ttccaaatgc ggctgatcgc ggcgaagagc tccttgtcac cggtttcggc cacaacatcc
     9361 gcagcaccgc aataaagata agcacctgtg acggcgtgcc ccaccgcgaa ctcctctttg
     9421 cgaagcggaa cacgatcctg ggtctgatcg ccggggtgcg gatcaccaat cttagctagc
     9481 tcggcaaagg cagaaggccc aggccagcga gctgatcccc gcatgttgat aaagatttcg
     9541 gaaagctcca agtatcggcg atccccggta acgcgataga gatccaccag acccatgatc
     9601 tgtgagggat tccaaccaaa gtgggccaac tggggtggtc gcggctggaa tgtggtgtat
     9661 agatagtcgg cacacttccg gccgaccttg aggaaactgt cctttcccgt tgcttgatag
     9721 tgaataaccg cggctgtgat caagtgaccc atgttgtaaa gctcatgata ttgcctcatc
     9781 ccccaacgtt ccttgtgacg attgaactgg gtttgtgtgc taatgtaacc atcgtcggcc
     9841 tgtgtgcggg caatcttctc gatccattca tccatcttgc gatcgagcgc ctcgtcgcgc
     9901 gttaccgcgt aaacacgggc catggcctcg atccatttat agacgtcacc atcactccag
     9961 ttatttccgc gatgctcggc ccccggctgc cccacgatct caaagttact taactgggca
    10021 ccgttggctg gatcgcgcat ggccctttct aaggcatcca gcgtctgatg ccaacaggtt
    10081 tcaaatcggc tggcccaaaa accgcttgtc caccgggcgg ccccaagttg tggcggctcg
    10141 aacttcgcat tcggcgaaat agccagtgta caaaccaata cagacgccgc tgttagccac
    10201 atcgtctctc tcctaattcg tttttttcct atccgcctaa tcctcaacac ccgcggccat
    10261 tgtattggac ctgtaacgag agtcaacgcg tcagcaaaac cagaggctaa acgacctgag
    10321 aagttattcg ccacaatggt tcatcgtctg tcgcggtgtg gcgttgcgga ctgacccaag
    10381 cggactgttc cctcaaacga acgcggtaac aaggaggcag aagacgtcac atccgccagg
    10441 cgatattgtg aatggatgct gattcattgt gctgaataag ttctcgggga gcgcctgctg
    10501 ctggcgcgct acgttccccg cgagccccta tttaagaccg ttgtcttttc aaaaccatct
    10561 gcagaaaaga ctgttctacg acttgaccca tctccataag atttgatggt cgtcccatag
    10621 taccaggcta cgaggtagca tccatgtcta agtctggcaa aaccaatgac cgtggtaaat
    10681 cactacgctg tcgtaacctc aagtctggca gttcgttgca actgattttg cggtaccgca
    10741 tcgtggcctt ttctcgcaag ccaacctgta aaaacaattg caatcatcgc cacagatcgc
    10801 gttttaaggc ttcttaacat cgggcaagtc ttgtgtgagg agatgttttc ggtaatcata
    10861 gcgtggacag cttgatggga gaatattatg aagtcaaaag agcacgtcca ccggttgtgc
    10921 gacgagaagt ggcatagtct cgaaatcgcc gatatttacg aaaggcttca atctgggccg
    10981 gacggcctgg accagcagga agcacagcat cggcttgcct attatggtcc caacacgctg
    11041 ccggccaaac agccgccgac gatctgggct attctgctgc accagattct caaccctttg
    11101 atattcattt tgctgggggc ggcagttgcc ttactagcca tcggcaaagg cgcggacgcc
    11161 atctttgttc ttatcgtcat tgccttgaac agtactctag gggcgtacca ggagtatcac
    11221 gccgaaaaaa gtgctgctgg tttgcgacgc ttgctcacca tcacggcgcg ggtccgcaga
    11281 aagggcaaag aaatagaagt tcccgcggaa gaggtggtgc cgggagatat tattctgctc
    11341 gagtcgggca acaaagtacc ggctgacatg cgtttggtag aggtcaacaa cctagcggcc
    11401 gacgaaagtt ttttgacggg cgaatccgtc gcctcggaaa agaccaccca catcctgccc
    11461 gaggacaccg ttgttgggga ccgcaagaac atggcctttg cgggatcgac cattactagc
    11521 ggcaggggag tcggaatcgt tgtcgccact ggcagtgaga ctcaagtggg ccttattgcc
    11581 aaaactgttt ccgaatcgga aatgggcaaa cccccgctgg tgttgcggat ggaacggttc
    11641 gtcaagcaaa tcaccatcct ggtgctggtg atcagcactt ttttggcggt gctgctgtgg
    11701 tttcagggca acgatattcc gtcgattttc tttttcgtgg tggccctggc agtttccgcc
    11761 atacccgagg ggctgccggt ggccctgact gtggcccttt ctgtggccac gagacggatg
    11821 tcggcgcgta atgtcatcgt gcgcaagctc acggctgtgg aaagcctggg aagctgcacg
    11881 gttatcgcca gtgataagac aggcacgctg acagtcaacc aacagaccgt tcgcgtcgtt
    11941 gatttggcgg atgggaggac gttcgagttt accggagagg gctatagcgg cgaaggacag
    12001 gtcttccgga agggcggcga gataacacca accgaccacg agcagttgac tcggctggcc
    12061 gaagcggcaa ttctggccaa cgaagggtct cttgtgcgcg aggaaggggg ctggaaacat
    12121 cgcggggatg ccatggatgt cgccttactg gccatgggtt acaagttggg cttgagtccg
    12181 accgaggcaa gacgccaagt taagatcctc ttcgagattc cgtatgagtc ggagcacaag
    12241 tattcggcgg tcttctacga gaaaaatggc acaacctatg tggccgccaa gggtgccgtg
    12301 gaaacaatcc tcgatttttg cagtggcgtc cttcgcgacg gaacgccgtt accgcttgac
    12361 cgcgacgata ttgaacggcg ggcacaagct atggcagaaa aaggtctccg tgtgttggcc
    12421 gttgccgggg gccaaactga aagtctccca tccgaagcct ccgatcaggc caagtttctt
    12481 tctgggctgt tgttttacgg attggtaggg tttatcgatc ctctgcgtcc cgaggccatc
    12541 gaagcggtga ggacatgcaa aagcgctggc atcaaggtgc tgatgatcac cggggatcat
    12601 cccgccaccg ccggtgccat agccagggaa ctgggcctgt cggacatcga tgagccggtg
    12661 gtgaccggag cccagttgaa tgaggcaggc cccacagaaa gcccggcttt tgaaaagctt
    12721 gtgacttcga gccatgtgtt tgcgagaatt tcgcccacgc aaaagctgga aattgttgat
    12781 gtcctgatgc gcgaggggga actcgttgct gttaccggcg acggggtcaa cgatgcaccg
    12841 gcgcttcgaa gggcgaacat tggtgtggcg atgggatcgg gaactgatgt tgctaagctc
    12901 gacttttgcc agtcttgagt gcctacgtgg cttggcagag ttggtcgaat
//
LOCUS       DAOSUT010000121        12599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2927_length_12599_cov_9.260204, whole genome shotgun sequence.
ACCESSION   DAOSUT010000121 DAOSUT010000000
VERSION     DAOSUT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12599
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_12599_cov_9.260204"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLD05_11250"
     CDS             complement(<1..246)
                     /locus_tag="PLD05_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78058.1"
                     /translation="MSGSRRRHKSEEKDKLIATRPRSRERPQDTPVETEARIIRRVRL
                     FFLAFVTVILGARPLFPSEGADLGDGVAVIMSLLGGIV"
     gene            complement(438..3173)
                     /gene="ppdK"
                     /locus_tag="PLD05_11255"
     CDS             complement(438..3173)
                     /gene="ppdK"
                     /locus_tag="PLD05_11255"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOP78059.1"
                     /translation="MSQAAKYVYYFGPEGTEGNASMVNLLGGKGANLAEMAGHPDLRL
                     PVPAGFTITTEVCTYFMQHGGKYPPGLEKQVEEALRKVEKAMGMKFGDPENPLLVSCR
                     SGARRSMPGMMETVLNVGLCTKTIPGLIKKTNNPRFVYDAYRRLIMMYSDVVMEKAEG
                     IEPPEGMGIRVQLERIMEKLKHEKGYEKDTDLTAEDLQKLCEEFKAKVKEVLGKPFPD
                     DPYEQLWGGISAVFKSWNGKRAVSYRRIEGIPDEWGTACNVQSMVFGNMGDTSATGVA
                     FTRNPATGENVFYGEWLPNAQGEDVVAGIRTPNPLNKATKTEQNRHLPSLEEAMPELY
                     RQLDEIRVRLEKHYKDMQDIEFTIQEGKLYMLQCRVGKRTGTAALNMAMDMLHEGLID
                     EETAVLRVAPKQLDELLHPVVDPNAEKKAKPIAAGLPAGPGGACGQIVFTSEDAVEWA
                     KKGKKVILVREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGKIKV
                     DVEKRCMTIDGKVLKEGDFVTLNGTRGLVYEGKLGLIDATENPRLKEFMALVEKYRKL
                     GVRTNADIPADAKVARDFGAEGIGLFRTEHMFYGEGSDEPLFILRKMILSRTAEERRK
                     ALDELFPFMKRDIKNTLEAMDGLPVTIRLLDPPLHEFVPQDEEKQAELAKAMGITVED
                     VKRRGEELHETNPMMGHRGVRLGITYPEITETQVRAIFEATAELLKEGKSPHPEIMVP
                     VTCDVKELDITKVIVDRIHAEVEKKFGVTIKYLYGTMIEIPRACLVADKMAKTAQFFS
                     FGTNDLTQMGFGFSRDDIGGFLPDYLEKRILMADPFQTIDQEGVGQLIEMAVQKGRAA
                     RPDLKIGICGEQGGDPESVEFCFRVGLNYVSCSPYRVPIARLAAAQASIKASRKK"
     gene            3636..4292
                     /locus_tag="PLD05_11260"
     CDS             3636..4292
                     /locus_tag="PLD05_11260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOP78060.1"
                     /translation="MSPEPPAAFDRRKHQLSRQFVLIGTLAECVLGLIGLALAWLFSI
                     PLRDHIQWNLAGVSKGLLATVPMLGVFCLVELAPGRTFQTLRTIVHMLIGTLVRSVGP
                     AELALLSVAAGLSEEVFFRGFIQAGLQQLLPEHGLVLGMPWESPVVAIVIAAILFGLA
                     HSLSLSYTILTTVLGIYLGTVFYATGNLLIPIVAHAVYDFIVLVYLAHVRRPRLGQGH
                     "
     gene            complement(4373..5848)
                     /locus_tag="PLD05_11265"
     CDS             complement(4373..5848)
                     /locus_tag="PLD05_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78061.1"
                     /translation="MKRFNVLACLAATVAVVNVGSSFLHGQELDFRAVRVEVDFGKKF
                     QVWDGFGFNYVEACQTRDYAANPQDYGGFSILDVTKKKEIIELVFGEEGLKVALIKMF
                     LDPFHQLRPGDPYDHETYTQNMRLFVREGLKTTRARGGDLTIITTLYGPPAYMTKQKV
                     LRGRDFDPQHKQDLVNYLVDWVRFLREKEGFPVKYISLHNEGEDWFRWTQAGLTDRDS
                     HDYNLFWPPEQVVEFVKLVDTALKQAGLEEVKVTPGETTNWYRFDHWGYADALAEDAE
                     ALRALGLITSHGFYSGTFGRWFGEHRSAGIDKLRGLRAELHAWVTSTSWGQMDAKNTK
                     EFHGNIYTAKVNGIIPWAGIQRPTKWTGGDPNPGCAFKVNEDGTYEIRRGYWMYKQLS
                     RAGQPGMVVAKATAMDSEIAVIAFASNGTPNPDAFVVINIGKQKLLKIRIVGSDAKAF
                     QVFRTTEDGKECYAPRGRLVCEENALVYEAPELSVTTFFAE"
     gene            5981..9274
                     /locus_tag="PLD05_11270"
     CDS             5981..9274
                     /locus_tag="PLD05_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78062.1"
                     /translation="MRISQTQNRTPVIALLFFGCVVFAFTVAWAESPRPWERQYSGEE
                     ATGPNVIALWQFLPGQETVDNSTHGHDLRLRGQSRFAGDGVFGNCLECFASTGQRDAG
                     NGAVVRNHPSLSPTGAFTLELWIKPKPELAECDLAFLLDKKYLHYPRETPQANRDYCL
                     YLRRTGPGKFRLVAYLGFGNDSAEFTSREVVLESGRWVHIAFSYDAAGVGRFFLNGEP
                     VGRTVHPGRGAIVPGQYDLVIGDRYGSLYWGFPGWIDQVRILNTLPDWAAGQLEIEPS
                     PLARTAFLRMEPNASVTLVLRNDTNREVMNAEISWDLADHRAKQTVSKIVPREEVELK
                     VPVDTRLRPGHYSLHVTAKATQENRIVEVTRDLRISVLPRPLPNTMPVIMWGTGDLDR
                     VVDIGFTHQLVSLADFDQIWKAETPTRAVPPGQLRERAEMLNEYLARGVGAIASLSPG
                     SWVVRNPKLQGQFQRVDRKGQPYKEANPCGLFPEVGAFCYRVGASGSETYRDFPAFQA
                     ALIHTEVRDSTNLCFHEHDLRAFRTKTGLEIPDLATSKWGVLYEKIAEFPANRVIPDD
                     HPLRTFYAWFWSEGDGWNDLHSAVHRGLKSARPDVWTFFDPAVRAPSIWGSGGEVDVI
                     SQWTYSYPDPIKIGQATDELFAMADGRPGQQVMKMTQIIWYRSQTAPNLPAEENRRAP
                     WEKEIPDARFITIAPDHLREAFWCKIARPIRGIMYHGWQSLVPAEHGAYRYTNPETEE
                     VLKDLIHRVVRPLGPTLLQVPDAPCDVALLESFTSQMFARRGTFGWGRGWEADMHLVL
                     QWARLQPRIVYEQTILRDGLEGFKILVLPACDVLPASVVEKVKDFQAKGGVVVGDEFL
                     CPAITPDIRITSYRRTGKAQEDKLALQEIADDLRGQLENRYARHFDSSHPDLVMRVRQ
                     FEKTAYVFVVNDHRTFGDYVGHHGLVMEKGLPLEGEVRLARSSGVVYDLVEHRRVTNH
                     AAGSMLAWTVQLGPGDGRVFMVTDRPIGGLKIEVPDQARRGDRVAIALRVLDDQGQPM
                     PALVPVELIVTDSQGQAAEPSGFYGAADGTVSVTLDLAPNDATGRWRIMARELASGQE
                     SSAELTVFE"
     gene            complement(9551..10987)
                     /locus_tag="PLD05_11275"
     CDS             complement(9551..10987)
                     /locus_tag="PLD05_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP78063.1"
                     /translation="MKNSRRSFLRCSMASPLFIASLGQRGTSAATTSLSEANSGSSVL
                     AEPARQIPVAGESDVIVCGAGPAGVAAAIAAARCGATVRLLEVHGCLGGIWTAGLLSW
                     ILDAGNKKGIIQEIIARLRMRDAVATYPEGASNAVGYRPEIMKWVLEEMIQEAGVCVQ
                     LFTRVCAAHVDDTKRLQAVITESKSGRQGWRAKVFVDATGDGDLAAFAGCRFDYGDEE
                     GGPAQPMSMLAMLVGLDPEAVKPFVRGLAEPAGERNPKGRLLEEIRRGGHDPSYHAPT
                     MFYLGHGLFCLMANHEYRVSALDAAEITAATFRSRAEVHRIVEALRSLGGPWKEIQIV
                     ATNEQIGVREGRRPYGLYRLTADDLKNGTRHEDAVCTVTFPVDIHSPDPEKNKGIVAR
                     GFRSQPYDIPYRALVAADVRGLLFAGRCISGDFVAHSSYRVTGNAAAMGEAAGVAAAL
                     AAAHHCDPKDVPWSEIASQLERLRSQAG"
     gene            complement(11190..12578)
                     /locus_tag="PLD05_11280"
     CDS             complement(11190..12578)
                     /locus_tag="PLD05_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78064.1"
                     /translation="MSRPVSGSSRREFLKTTGQLAAATALAGSMIPHVHAVEDNTLRI
                     ALIGCGGRGTGAAANAMSVKRVPIKLVAMADVFPNRLASSYEQLKNRFGDQVDVPDDR
                     KFIGFDGYRKAMDCLRPGDVAIFATPPAFRWVHFTYAIEKNLNVFMEKPVTVDGPTSK
                     RMLELAQKSVEKNLKVGVGLMSRHSRALQELFKRIQDGAIGDVIFQRGYRMHGPIGFF
                     ASTPKPEGISELLYQVQRFHSFIWASGGCYSDFYIHIIDHLAWMKNDWPVKAMGLGGR
                     HYKTSPEGVPYIDQNFDVYAVEYTYADGSKFCFDGRCMGGADNVYYSYIHGSKGSALV
                     SASGDCGLPSRIFRGQNPDMSALEWESKVPPEERDPYQNEWNALVDAILDDKPHNEAE
                     RGVKASLVTSMGRMACHTGREITYEEILNHSHEMAPGLDQLTMDSPAPLQLGPDGRYP
                     VPMPGLKTDREY"
BASE COUNT         2639 a         3687 c         3447 g         2826 t
ORIGIN      
        1 gacaatccca cctaaaaggg acatgatcac ggccacgccg tccccgaggt cggcaccctc
       61 gctagggaaa aggggcctgg cccccaggat caccgtaaca aacgccagga agaacaatcg
      121 gacccgccgg ataatgcgtg cctcggtctc caccggcgta tcctgtggcc gctcgcgcga
      181 acgtggccgt gtggctataa gcttatcttt ttcttcgctc ttgtgacggc gtctggaacc
      241 cgacatcgcc taaattcaag tatcccgcag gtggtgatgg ttgtttcctc tatgctttag
      301 cttacaccac aaagctgggg acgacatcgt tgggcatgtt cggcaaacga tttgaagaaa
      361 aaaggctaca ggaagtcgtt ggtaacacct cctgtagcct gttttacaat ccagcctaga
      421 cggcgatgtc tagaacatca cttcttgcga ctggccttga tggaggcctg agcggctgcc
      481 aaccgggcaa tcggcacacg atagggcgag cagctcacgt agttcaggcc gacacggaag
      541 cagaactcga ccgactcggg atcaccgccc tgctcaccgc agattccaat cttcaggtcc
      601 ggtcgagccg cccgaccctt ttgcacagcc atttcaatca actggccaac gccttcctga
      661 tcgatggttt ggaagggatc agccatgagg attcgcttct cgagataatc cggcaggaag
      721 ccgccaatgt catcccgact gaagccgaag cccatctgag taaggtcatt ggtgccgaag
      781 gagaagaact gagctgtctt ggccatcttg tcggccacca agcaggcccg tgggatttcg
      841 atcattgttc catagagata cttgatcgta accccaaatt tcttctcaac ctcggcatga
      901 atccggtcca caatgacctt ggtgatgtcg agttccttca catcgcacgt gacgggcacc
      961 atgatctccg gatggggcga cttcccttcc ttgagcaatt ccgccgtggc ctcaaagatg
     1021 gcccgcactt gcgtctccgt gatctcggga taggtgatgc cgagacgcac accgcggtgg
     1081 cccatcatgg ggttggtttc gtgaagctct tcaccacggc gtttcacatc ctcaaccgtg
     1141 atgcccatgg ccttagccaa ctcagcctgc ttttcctcat cctggggaac aaactcgtga
     1201 agtggcgggt ccagcaagcg aattgtcacc ggcaacccgt ccatggcctc gagggtgttc
     1261 tttatatccc gcttcatgaa ggggaacaat tcgtccaggg cctttcggcg ttcttcagcg
     1321 gtccgcgaga gaatcatctt ccgcaggatg aaaagcggtt cgtccgaacc ttctccatag
     1381 aacatgtgct cggttcggaa gaggccaatg ccctcggctc cgaaatcccg ggcgactttg
     1441 gcgtctgccg gtatatcggc attggtgcgg acgcccagtt ttctgtattt ttccacgagg
     1501 gccatgaact ccttcagacg cgggttctcc gtcgcatcaa tgagaccgag cttaccttca
     1561 tagacaaggc cgcgcgttcc gttgagcgta acaaaatcac cctccttgag caccttgccg
     1621 tcaatcgtca tgcaccgctt ttcaacatcg actttgatct ttccggcacc gacaatacag
     1681 cacttacccc aaccgcgggc cacaagggct gcgtggctgg tcataccacc acgggccgtc
     1741 aggatacctt ccgccgcccg cataccttcc acatcctccg gattggtctc ctcccggacg
     1801 aggatgacct ttttcccttt cttggcccac tccacggcgt cttcgctggt gaagacgatt
     1861 tggccacaag caccacccgg cccggcgggc aaaccggccg cgatcggctt ggccttcttc
     1921 tcggcgttgg ggtccaccac cgggtgaaga agctcgtcaa gctgcttggg ggcgacacgg
     1981 agaacggccg tctcttcgtc gatcaggccc tcgtgaagca tgtccatggc catgttgagg
     2041 gcagccgtcc ccgttcgctt gccgacacgg cattgaagca tgtagagttt gccctcttgg
     2101 atggtgaact caatgtcctg catgtccttg tagtgcttct caagccgcac ccggatctcg
     2161 tccaactggc gatacaactc cggcatggct tcctccaggg aggggaggtg ccggttctgc
     2221 tcggtcttgg tcgccttgtt gagtggattg ggcgtgcgga tacccgccac cacgtcctcg
     2281 ccctgggcgt tcggcaacca ttcgccgtag aacacgttct cgccggtcgc cggatttcgt
     2341 gtaaaagcca ctcccgtggc cgaggtgtcg cccatgttcc cgaagaccat gctctgcacg
     2401 ttgcaggcag ttccccattc gtcggggata ccctcaatac gacggtagga aacggcccgc
     2461 ttgccgttcc agctcttaaa gaccgcggaa atacctcccc aaagctgttc atagggatca
     2521 tcggggaatg gctttccgag cacttccttg accttcgcct tgaactcttc gcagagtttc
     2581 tgcaaatcct cggcggtcaa atccgtgtcc ttctcgtagc ctttctcgtg cttaagcttt
     2641 tccatgattc gctcgagctg gacccggatg cccatccctt ccgggggctc gatcccctcg
     2701 gccttttcca tgacgacgtc ggagtacatc ataatgagcc ggcggtaagc gtcgtacacg
     2761 aaccgaggat tattcgtctt cttgatcaac cccggaatcg tcttggtgca gaggcccacg
     2821 ttgagaactg tttccatcat gcccggcata gaacggcggg cacccgaacg gcaggagacc
     2881 agcaacggat tctccgggtc cccgaacttc atacccatcg ccttttcgac tttcctgagg
     2941 gcttcctcta cctgcttttc caatcccggc ggatatttcc caccatgttg catgaagtaa
     3001 gtacatactt ccgtggtaat cgtgaaccca gccgggacag ggagccgtag gtcaggatgc
     3061 cccgccatct ccgccaaatt cgcccccttg ccaccaagga ggttgaccat cgaggcgtta
     3121 ccttccgttc cctccggacc gaaataatag acgtactttg ccgcttgtga cattgttcaa
     3181 tctcctaagt gacttaccgc acaaacaacc aaaccgcttg cacccccacg accgcaaccc
     3241 ctttcgggta gtctggacta ccaccgcaca gcgctcgttg acgcgacgca ggacactggt
     3301 gcactttcca aaatagaatc ctgtagttta accctaggcc aggagagggt caacgaaccc
     3361 cgtccgatgg gtcaatgtcc attacaagga taggtgaatt cacaatgacg cgcatccttt
     3421 tggaaacaca gattcttccc ggctttcccg atgacgctga tggtccggat tgacaaatcc
     3481 cttttactgc aaatgacact ggacctatgc ctcaggaaat ccgaaaattg agtttttctt
     3541 acgattgcgc tcagaacttt gttcaaaggg gctacatggg gctgaatgac tttgtttagc
     3601 ctgagtcccg aatccccgaa ccgcctttca gtattatgtc gccagaacca cccgcagctt
     3661 tcgatcggcg taaacatcaa ctttcacggc agttcgttct catcgggacg ctggccgaat
     3721 gcgttttggg attgatcggc ttagcccttg cgtggctttt ctccatcccg ctgcgggatc
     3781 acattcagtg gaatctagcg ggcgtcagca aagggctgtt ggccactgtc cccatgcttg
     3841 gtgttttctg tttagtggaa ctggctccag gcaggacatt tcaaacgctt cgcaccatcg
     3901 tccatatgct gattggaaca ctggtccgtt ctgtgggccc agcagaactg gccttgctat
     3961 ccgttgcagc gggattgagc gaggaggtgt tttttcgggg atttattcaa gcggggcttc
     4021 aacagctcct tcctgaacac gggctcgtcc ttgggatgcc ttgggagagt cctgtcgtgg
     4081 ccatcgtcat tgccgcgatc cttttcggct tggcccactc tttgtcgctg tcctatacca
     4141 tcttgaccac ggtcttggga atctatctcg gcaccgtttt ctacgcgacc ggcaatcttc
     4201 tgataccgat tgtggcccat gccgtatatg actttattgt cctggtctat ctggcccacg
     4261 tccggcgccc acgtctgggc caaggtcact aatgccgcgg acctcacatc tcatccgaag
     4321 gctctctggc tcgttagccc atctctccac agacaaaaag acattcgccg ggttattcgg
     4381 cgaagaacgt ggtcaccgat agctccggcg cttcgtacac caaagcgttt tcctcacaga
     4441 cgagacgtcc tcgaggcgcg taacactctt taccgtcctc ggtggtgcga aagacttgga
     4501 acgccttggc atcgcttcct acaatccgga ttttcaagag cttttgtttg ccaatgttga
     4561 tgacgacgaa ggcatctgga ttaggagtgc cgttggaggc gaaggcgatc acggcgattt
     4621 cgctgtccat agcagtcgct ttggcaacta ccatccccgg ttgaccagcc cgcgacagtt
     4681 gtttgtacat ccagtacccc cggcgaattt catatgttcc gtcctcatta actttgaagg
     4741 cacagccagg gttggggtca ccgcccgtcc actttgttgg cctttgaata ccggcccaag
     4801 ggataatccc gttgaccttg gccgtgtaga tattgccgtg aaactccttg gtattcttgg
     4861 cgtccatttg tccccagctt gtggaagtta cccaagcgtg gagttccgct cgtaaaccgc
     4921 ggagcttatc aatcccagca ctccggtgtt cgccaaacca acggccgaag gttccgctat
     4981 aaaagccgtg cgaagtgatc aatcccaatg cgcgaagggc ctcggcgtct tcggcgaggg
     5041 cgtcggcata gccccaatgg tcaaagcgat accaattcgt ggtttccccc ggtgtgacct
     5101 taacttcttc cagaccggcc tgctttaacg cggtatcgac caacttcacg aattcgacaa
     5161 cctgctctgg tggccagaac aagttatagt cgtggctgtc acgatcggtt agaccagcct
     5221 gagtccagcg gaaccagtcc tctccttcgt tatgtaaaga gatgtatttg acagggaagc
     5281 cttccttttc acgaagaaag cggacccaat cgaccaaata gttcacaagg tcctgtttgt
     5341 gctggggatc gaaatcccgg ccgcggagaa ccttttgttt tgtcatgtag gctggtgggc
     5401 cgtaaagcgt tgtgatgatc gtcaagtctc cgcccctggc ccgcgtggtc ttgaggccct
     5461 cacgaacaaa aagccgcata ttctgggtgt aagtctcgtg atcatacggg tcgcccggcc
     5521 gaagctggtg gaacggatcc aagaacatct tgatcaaagc aaccttaaga ccctcttcgc
     5581 caaagacgag ctcaatgatt tcctttttct tcgttacgtc gaggatcgaa aacccgccgt
     5641 aatcttgcgg gtttgcagca tagtcgcggg tctggcacgc ctcgacatag ttaaatccga
     5701 agccatccca gacttgaaat ttcttgccaa agtcgacttc cacacgaacg gcgcggaaat
     5761 ccaattcctg cccgtgtagg aacgagcttc ctacattgac cacagctacc gtggcggcaa
     5821 gacaggctaa cacattaaac cgtttcatgg cactgccact cccattatca ccttgttgaa
     5881 tgctaaacga tgcaacaata tcctaatggg caatcgctct caatttacag gacacaggcc
     5941 acaacccaga tcacagcttg acttaattag ggaggaacac atgcgaatat ctcaaacgca
     6001 aaataggact ccagtcattg ctttgttgtt ctttggatgc gtcgtgttcg cctttaccgt
     6061 ggcatgggcg gagtctccac gtccctggga gcggcaatat tctggcgaag aggccacagg
     6121 cccaaacgtg attgcattgt ggcagttttt gcccggccag gaaacagtgg acaattccac
     6181 acacggccat gacttgcgac tccggggcca atcacgcttt gccggagacg gggtcttcgg
     6241 caattgtcta gaatgctttg cgagcacagg ccaaagggac gccggaaacg gcgcggtggt
     6301 aagaaaccat ccgtcgttaa gcccaaccgg ggcgttcact ctggaattat ggatcaagcc
     6361 gaagccggaa ttggccgagt gcgatctggc cttcttgttg gacaaaaagt acctccacta
     6421 cccacgggaa acgccccaag caaaccgtga ttactgcttg tacctacggc ggacggggcc
     6481 gggcaagttt cgcttggtgg cgtatctcgg ttttggcaat gattcggcgg aattcacttc
     6541 ccgcgaggtt gtgctggagt ctgggcgctg ggttcatatc gccttttcct acgatgcggc
     6601 gggcgtcgga cgattctttc tcaacggaga acccgttggc cgaacggttc acccagggcg
     6661 cggggccatc gtgcctggtc aatatgattt ggtaattggc gatcgctacg ggtctcttta
     6721 ctggggcttt cccggctgga ttgatcaggt cagaatcctc aacacgctgc cggactgggc
     6781 agcggggcaa ctggagatcg agcctagccc cctcgcaaga acggccttcc tccgcatgga
     6841 gcccaacgcc tctgtcacgc tcgtcctgcg caacgataca aaccgggaag tgatgaatgc
     6901 cgaaatctcc tgggatctgg ctgaccaccg ggcgaagcaa acggtctcca agattgttcc
     6961 acgtgaggaa gtcgaactga aggtccccgt ggacactcgg ttgcgcccag gtcactattc
     7021 gctccacgtt acggcgaagg caacgcagga gaatcgaatc gtagaagtca cccgggacct
     7081 gaggatatcc gtcttgccca ggccccttcc caacaccatg cccgtgatca tgtgggggac
     7141 aggggacctc gatcgcgtgg ttgatattgg ttttacgcat caactggtaa gcctggcgga
     7201 cttcgatcag atttggaaag ccgagacacc aacgcgggct gtaccacccg ggcagctcag
     7261 ggaaagggcg gagatgctta atgagtattt ggcacgaggt gtgggagcga tcgctagctt
     7321 gtcgccaggg tcgtgggttg ttcgcaatcc aaagttgcaa ggccaattcc aacgggttga
     7381 tcgaaaagga caaccctaca aagaggccaa tccttgcggt ctgtttcccg aggtcggcgc
     7441 cttttgctat cgcgtggggg catcagggag cgagacatat cgtgactttc ccgcttttca
     7501 ggcggcgctc attcacaccg aggtccgtga cagcaccaat ttgtgtttcc acgaacacga
     7561 cctccgggcg tttcggacca agaccggttt ggagattcct gatcttgcca ccagtaaatg
     7621 gggcgtgctc tacgagaaga ttgccgagtt tccagcaaac cgcgtgattc cggatgatca
     7681 tccgttgcga actttttatg catggttctg gagtgaaggt gacggctgga acgacttgca
     7741 ttcggcggtc catcggggcc tgaaatcggc gcggcctgac gtgtggacat tttttgaccc
     7801 tgccgttcgt gcccccagta tttggggcag cgggggcgaa gtggacgtca tttctcagtg
     7861 gacatacagc tatcccgacc ccatcaaaat tgggcaggct acggatgaac tgttcgcaat
     7921 ggctgatggc cgtcccggtc aacaggtgat gaagatgacg caaatcatct ggtatcgttc
     7981 gcagaccgct ccgaatttac ccgccgagga aaaccgccgt gccccttggg agaaggagat
     8041 tcccgacgca cggtttatca ccattgctcc ggatcatctt cgggaggcgt tttggtgcaa
     8101 aatcgcccgg cccatccgcg gcattatgta tcacggatgg caatcgctcg tcccggcaga
     8161 acacggtgcc taccgctaca ccaacccgga aacagaagag gtcctgaaag acctcattca
     8221 ccgtgtggtg agaccgctgg gaccgacact gcttcaggtt ccggatgccc catgcgatgt
     8281 agcccttctg gagagtttca cttcgcaaat gttcgccaga cgcggcacgt tcggttgggg
     8341 acggggctgg gaggccgata tgcacctggt ccttcaatgg gctcgcttgc agccacggat
     8401 cgtctatgag caaacgatct tgcgcgatgg gcttgaagga ttcaaaattc tcgtcctgcc
     8461 tgcgtgcgat gtcctgccgg catcggtcgt ggagaaggtc aaggactttc aggcgaaagg
     8521 tggtgtggtc gtgggcgatg agttcctatg tcccgcgatc acccccgata ttcgaattac
     8581 ttcctatcgg cgtacgggca aagcccagga agacaagctg gccctgcaag agatcgcgga
     8641 cgacctccgg ggtcaactgg agaaccgcta cgcgcgtcac ttcgattctt ctcaccccga
     8701 tctggtgatg agagtgcggc agttcgaaaa gacggcgtat gtgttcgtgg tgaatgacca
     8761 tcgcacattc ggcgattatg tgggtcatca cggattggtc atggagaagg gactcccatt
     8821 agaaggtgag gtccgattgg cgaggtcatc cggtgtggtc tatgaccttg tggagcatcg
     8881 tcgagttaca aaccatgcgg ccgggtcgat gcttgcttgg acggtacaac ttgggccagg
     8941 cgatggccgg gtgttcatgg ttacagatcg gcccatcggc gggttgaaaa tcgaggtgcc
     9001 cgatcaagcc cgcaggggcg accgcgtagc cattgccctt cgcgttttgg acgatcaggg
     9061 ccagcccatg cccgcgctcg tgcccgtcga gctcatcgtc accgactctc aagggcaagc
     9121 tgcggaaccg agcggcttct atggtgccgc cgatggcacg gttagcgtca ccctggattt
     9181 agccccgaat gatgccaccg gcagatggcg aattatggcg agggaactgg cctccggtca
     9241 agagagctcc gctgagctga cggtctttga gtgacttctc acccgcagtg caacttagtg
     9301 caacttagag cctatcccac aacctcccct cgcctgctcg gcgggagagg ggccggggtg
     9361 agggtggctc gaatcgctgc tggttttttg gatcgactcc gacgatcgtt tgccctcacc
     9421 ctaaccctct cccgggggga gagggaactt tgggacagtc tccaaggcta tcgcttcacg
     9481 tgagcgagca gcagcgcacg gccggaccgg ttgggcactc gctgccgatt ttaaggcttc
     9541 ggcatcgcta ttacccagcc tgggaacgta gccgctctaa ctgcgaggca atttccgacc
     9601 agggaacatc tttggggtcg caatgatggg ccgcggcaag cgcagcagcc acgccggcgg
     9661 cctcacccat agcggcggcg ttacccgtca cgcggtagct ggaatgagcg acgaaatcgc
     9721 cgctaatgca tcgccctgca aataaaagac cacggacgtc agcggccacc aatgctcggt
     9781 acggaatatc atacggctgt gagcgaaatc cccgggcgac gatgccttta ttcttttccg
     9841 ggtcgggcga atgaatgtcc acgggaaagg tgaccgtgca gacggcgtct tcgtgccggg
     9901 tcccgttctt gagatcatca gccgtcaagc ggtacaaacc gtagggacgg cggccctcgc
     9961 ggacgccgat ctgctcgttt gttgccacaa tttggatttc cttccatggc ccgccgagag
    10021 atcgcaaagc ctccacgatc cgatgaactt cagcccgcga acgaaacgtg gccgctgtga
    10081 tttcggcggc atcgagggcg gatactcggt actcgtgatt ggccatcaga cagaacagac
    10141 cgtgccccag ataaaacatc gtcggggcgt ggtaagacgg atcgtggccg ccgcggcgga
    10201 tttcttcgag cagccgtccc ttcgggttac gttcgccggc aggttcggca aggccccgca
    10261 cgaaaggctt gacggcctcg ggatcaagtc ccaccagcat ggccagcata ctcatgggtt
    10321 gggctggccc tccttcttcg tccccatagt cgaatcggca gcccgcaaag gcagctagat
    10381 ctccgtcgcc ggttgcgtca acgaacacct tcgcccgcca gccttggcgt ccagacttgg
    10441 actcggtaat cacggcctgc aaccgcttgg tatcatccac gtgcgcggcg cagacccgcg
    10501 taaaaagttg cacgcacacg cccgcctctt ggatcatctc ttcgagtacc catttcatga
    10561 tctccgggcg atagccgacg gcattgctgg cgccctcggg ataagtggca accgcatccc
    10621 gcatgcggag gcgggcaatg atttcctgga taattccctt tttgtttccc gcatccaaaa
    10681 tccaagagag aaggcccgca gtccaaatgc ctcccaggca tccgtgaact tccagcagtc
    10741 gcaccgtggc gccgcaccgt gcagcggcta tcgccgcggc aacaccggcc gggcctgccc
    10801 cgcaaacgat gacatcactt tccccggcca caggaatctg ccgggcgggt tcggcaagaa
    10861 cagaactacc ggaattcgcc tcggacaaag atgttgtcgc cgcactggtt ccacgttgtc
    10921 ccagcgaagc aatgaaaagg ggacttgcca ttgaacaacg caaaaagctc cgccgcgagt
    10981 tcttcatcgt tatacctttc ctgatgcgtt gacaacacca gtgtcgcttg aaggcttccg
    11041 ctatgcatca gatcgcacaa aaaaggatga taagaacaca ccggtgtgct cttatcatcc
    11101 aaatctccat gcgcaagtca agctttgaca cggcgcccac gccggggata tccgttttgg
    11161 gaatccacaa tgatcctgtc gcttcgaaat caatactcac gatcggtttt cagaccaggc
    11221 atggggacgg ggtagcggcc gtcggggcca agttgcaacg gcgccgggga gtccatcgtc
    11281 aattgatcca ggccaggcgc catttcgtgg gaatgattca ggatttcctc ataggtgatt
    11341 tctcggcctg tatgacaagc cattcgcccc atcgaagtga caaggctggc cttcacgccg
    11401 cgttcggcct cgttgtgggg cttgtcgtca agaatggcgt caaccagggc attccattcg
    11461 ttttgatagg ggtcgcgttc ttcgggcggg accttggatt cccattccag ggccgacatg
    11521 tcaggattct gtccccggaa aatccgggac ggcagcccgc agtccccgct ggccgagacc
    11581 aaagccgacc cttttgaacc gtgaatgtag ctgtaataca cgttgtcggc cccgcccatg
    11641 caacgaccgt caaagcagaa cttgcttccg tcggcatagg tgtattccac agcgtacacg
    11701 tcgaagtttt gatcgatgta gggcacaccc tcggggcttg tcttgtagtg gcgaccgccg
    11761 agacccatgg ccttgaccgg ccagtcgttc ttcatccagg ccaggtgatc gatgatgtga
    11821 atgtaaaaat cgctatagca accaccgctt gcccaaatga agctgtggaa tcgctgcacc
    11881 tgatagagaa gctcgctgat tccctcgggc tttggggttg aagcaaagaa tccgatgggg
    11941 ccgtgcatgc ggtatccgcg ctggaaaatc acatcgccga tggcaccgtc ttggatgcgt
    12001 ttgaacagct cttgcaatgc ccgactgtga cgggacatta gccccacgcc gaccttaagg
    12061 ttcttctcga cagatttctg agccagttcg agcatccgtt tgctggtagg accatcgacc
    12121 gtgacgggct tttccatgaa gacgttcagg tttttctcga tggcataggt gaaatggacc
    12181 cagcgaaacg ccgggggcgt cgcaaagatc gccacatccc caggccgcaa gcaatccatg
    12241 gccttccgat aaccatcaaa cccgatgaac tttctatcat caggcacatc gacctgatcg
    12301 ccaaatcggt ttttcaactg ctcgtaacta gaagccagtc gattggggaa aacgtcggcc
    12361 atcgctacca atttgatggg cacccgcttt acagacatgg cattggcggc ggccccggtt
    12421 ccgcgtcccc cgcaaccgat gagggcgatg cgaagggtgt tgtcctcgac ggcgtggaca
    12481 tggggaatca tgcttcccgc cagcgccgtg gctgctgcca actggccggt tgtcttcaag
    12541 aattcacgtc gcgacgaacc actgacagga cgactcataa gaaacctcct cggcgttca
//
LOCUS       DAOSUT010000122        12580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2932_length_12580_cov_8.565429, whole genome shotgun sequence.
ACCESSION   DAOSUT010000122 DAOSUT010000000
VERSION     DAOSUT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_12580_cov_8.565429"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLD05_11285"
     CDS             complement(<1..466)
                     /locus_tag="PLD05_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HOP78065.1"
                     /translation="MKVESQVFGTMPSGEVIPLYTLDNGRGLVVRVMSLGATLTAVEF
                     PDREGKRENVTLYLESLEDYLKGHPCFGSICGRYANRIAKGQFQLDGVTYQLATNNGP
                     NHLHGGKVGFHQRVWDHRPVQGDGWVGVEFSRVSPDGEENYPGTLTVSVTYRV"
     gene            complement(715..>1785)
                     /locus_tag="PLD05_11290"
     CDS             complement(715..>1785)
                     /locus_tag="PLD05_11290"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOP78066.1"
                     /translation="DTVVAQRTSSSKKYRDAVAVALGLLQELHFVASPEEIPAVAHRL
                     IHGGEAIREPVIITQQIKEIIRQFIPLAPLHIPAGLQAIEVTESLFPGAVQTGIFDTA
                     FFAHIPPAAYLYPVPYEWYTEWGIRRFGYHGTSHAYAMERAAQMLGRPVESVHLITCH
                     LGQGSSATAIAEGKAVTNTMGFTPLEGFMMGTRSGTVDPGIITYVQRHHGLTADQVER
                     ILHTQSGLLGVSGISGDYREVLRAAQAGHKRSQLALEMYAYRVRAMLGALAVTLGRVD
                     ALVFTGGIAENASELRADICRGLECLGLFLNPERNAACQPDADISSDNSPGRILVIRS
                     QEDYILAREAKRLVSANRAGFA"
     assembly_gap    1786..1885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1886..2574)
                     /locus_tag="PLD05_11295"
     CDS             complement(<1886..2574)
                     /locus_tag="PLD05_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOP78067.1"
                     /translation="MQTAQILGQRLAVVLLVGSLVVTGCSKKQAPQESQTNVAAAQQG
                     SGSAPSEIPVEPMNPSAPVAESVAPGRSEESSQPVEIPKVVMAEIDRAKCNLFVGDSA
                     PHGELSQLSGGTQQISDLFEKKATVLVFWTSGAERYAALNAVNLLGDMQSEIESKYRD
                     QGVAVLGIHVGPKSEETEKIIQESGANFTILVDTDGGFFRQFSAHQPPCLYLLDGSGR
                     IVWLDVEYSRSA"
     gene            complement(2624..4390)
                     /gene="aspS"
                     /locus_tag="PLD05_11300"
     CDS             complement(2624..4390)
                     /gene="aspS"
                     /locus_tag="PLD05_11300"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOP78068.1"
                     /translation="MLRTHTCGELRTSHIGQHVTLCGWVDTYRDHKGVVFVDLRDRYG
                     KTQIVFSSDNGQELHQIGRSLRPEYVIQVTGLVERRPEGTENPKLATGEIEVRAEQCC
                     ILNRANVHPFLPSTPDLPGEEVRLRYRYIDLRRPQMQQILLLRHRMIKVMRDYFDEHG
                     FIEVETPMLGRSTPEGARDYLVPSRIQKGTFYALPQSPQLYKQILMVAGYDRYMQIAR
                     CFRDEDLRADRQPEFTQLDLEMSFVQADDVMGIVEGLMARLFREILGQELTLPLPRMT
                     YDEAMERFGHDAPDLRFGMELTDITAIAAECDFRVFREAAENSKRVRGITVPGGANRY
                     SRKQIDELTEWVKQDFGAKGLVWFRVEPEGTLNSTVAKNFTASQLEAIRAKMGAQPTD
                     LLLFVADDFEITCKALYGLRKRFGAELKLYDPRAMHLSWVVEFPMFAIDEEEGGWTSM
                     HHPFTAPRPQDRELLKTDPGKCRAQAYDLVLNGTEVGGGTIRIHDSELQQEVFQLLGI
                     DEAKAKDRFGFLLEALQYGAPPHGGIALGVDRLVAIFAGVDSIRDCIAFPKTQRAVDL
                     MTGAPSEVDPKQLRELGIKIEK"
     gene            4925..6634
                     /locus_tag="PLD05_11305"
     CDS             4925..6634
                     /locus_tag="PLD05_11305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOP78069.1"
                     /translation="MAGLLQAEGPDRGKIYPIEKNCVILGRHPNCDIVLEVGAVSRQH
                     ARIVQTASGFFLEDLNSRNGTFLNEQRVHGRQPLKDGDRIRICDLVFSFFEAAPPKKE
                     GSETEITDAAWVDDEERFRRDSTIVSRFDVTATGSRVEVNPEAKLRALMEISQNLGKA
                     LSVDEVLDKILTSLFNVFTQADRGFIILVDPQTGKLVPRAVKTRRPEDREQLRISRTI
                     VNAVTSTKQAILSADVATDSRFDLSQSIVDFPIHSVMCVPLVGSDRKVLGVIQMDTRQ
                     RKARFTPEDLEVLVSAASQVAVFVENAILHDASMREELLRRELALAHKVQQGFLPSEP
                     PHVPEYDFFDFYDPAHEVGGDYYDYIPLPANRLALIMADVSGKGIAAALLMAKLSAEI
                     KYCLASEASPQTALRRLNKVFCSDRWDDRFITLILVVLDPVTHHYWLVNAGHWPALLR
                     KANGEILVVGAEKAGPPLGVSLDASYDFHVGTFDPGDLLILFTDGVVDAMNGNGEAFG
                     NERLMNVLQRASGSPEEVGNSLVQAVHRFVGQQPQADDMCLVIVGRQAKDRDDKIVAN
                     NVS"
     gene            complement(6757..7539)
                     /locus_tag="PLD05_11310"
     CDS             complement(6757..7539)
                     /locus_tag="PLD05_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOP78070.1"
                     /translation="MLPRLNVYAVPSLADPKELETGTVVVIDILRATTTIVHAIAAGA
                     TAVVPCASVEDAQRLAEILHETDDTTSVLLAGERDCLPIPGFDLGNSPSEYRPEVVFR
                     AVIILTTTNGTQAFQVCRRAQRVLVGAFVNASAVLKALFEESHAVHLLCSGIEGEFTR
                     DDTLFAGMLVDHLERKSGLRYELNAQAITARENWRAAFAPPYSSGAEPIPPEMLAAQL
                     RNSAAGEKLIAAGMESDIYDAARIDLFTCLPTFDPTSGIIVG"
     gene            7854..8522
                     /gene="ubiE"
                     /locus_tag="PLD05_11315"
     CDS             7854..8522
                     /gene="ubiE"
                     /locus_tag="PLD05_11315"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOP78071.1"
                     /translation="MFAEIAPRYDLLNHLLSFGTDVYWRDRTVKSSEVLGTGPVLDVC
                     CGTGDLTLAFWRHRRNSFPIVGVDFCREMVQLAKRKADRRHAQIHFIEADGLHLPFPD
                     DTFAVVAVAFGLRNMADTGRGLSEMVRVCRPGGEVMVLEFTLPTLFPINVIYRAYFRF
                     LLPIIGQALAPNRQEAYNYLPASVHEFPQGEALLAEMAKAGLKDLVLRPFSFGIATLY
                     KGSK"
     gene            8519..9205
                     /locus_tag="PLD05_11320"
     CDS             8519..9205
                     /locus_tag="PLD05_11320"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOP78072.1"
                     /translation="MKRQIVVAITGASGVTYAIRLIEVLLAAACDVHLTISRAGRTVL
                     KEELDLTVDLDNFQPSSLLLDSQDSAEDTKLRLLRGMAGVAGESSHVLGVPIGEPGTL
                     YYHHFKRLTAPIASGSFITHGMVICPCSMSTLSTIVHGGGNNLIHRAAEVHLKEGRKL
                     ILVPRETPLSLIHLRNLALAAEAGATVLPAMPAFYHNVLTVRDLVDFIVARIIDQLGI
                     ENELIRRWGT"
     gene            9336..10199
                     /locus_tag="PLD05_11325"
     CDS             9336..10199
                     /locus_tag="PLD05_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOP78073.1"
                     /translation="MRKVRHFLELIRFSHTLFALPFAALSAAMVWKVVPFQWHHLLGF
                     LLCMVFARSTAMAFNRLVDRKLDAANPRTAGRHIPRGVLGVREVWGFTAVCAIGFVLS
                     TLLFLPNYWPLVASGPVLAFLCGYSYSKRFTWLSHFWLGTALALAPPGIWLAIQGTLA
                     WPPILLACAVIFWVAGFDIIYACQDYDFDRRQKLWSIPAVFGIRRALWLAAASHGVMV
                     GCLAMLPILFNEFDWLYWVGLALVSVLLIYEHWLVRPDDLRRVNEAFFHVNAVVSVGL
                     LIIGTVDIWLL"
     gene            10224..11393
                     /gene="mqnE"
                     /locus_tag="PLD05_11330"
     CDS             10224..11393
                     /gene="mqnE"
                     /locus_tag="PLD05_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOP78074.1"
                     /translation="MLDQNIVRTERLVESRLDEIRNKIEAGRRLSFEDGVFLFQPSVS
                     LHVVGQLADLVCRRRHGRTVYYNLNTHLNPTNICGYRCKLCAFFRSKGDPDAYIMSQE
                     EILKRAEDADQAGCTELHIVGGLPPGIPFEWYLGIVEAIHNQYPRLHLKAWTAVEIAW
                     FARISGLSVKEVLLRLIDAGLGSLPGGGAEIFHPEVRRQISSHKADGKTWLDVHRTAH
                     SLGLKSNATMLFGHVETIYHRVDHLLELRTLQDETGGFQAFVALPFHPVRTAFSHLPK
                     LSAFDCLRTIAVSRLLLDNFDHIKAYWVALGIGVAQTALEYGANDLDGTVRYENIYHD
                     AGADSPDVLSVEELCALIRETGREPVERDSLYRRVRRFGREWELEDNHPDVRNRE"
     gene            11457..>12580
                     /locus_tag="PLD05_11335"
     CDS             11457..>12580
                     /locus_tag="PLD05_11335"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOP78075.1"
                     /translation="MMNEGTKPSTISPRDQDPLRLPIQFVKGVGPRRAEILAKLGIET
                     VSDALFFFPRSYEDCSRVRPVNQLEEGTVQAVVGVVEDVESRVLRGGQTVVGVLIAGE
                     GGYVRAVWFQQPYRRDYFREGQRVMAVGKPRRNGLIWEMVHPRVIVLGEEEQPKLAPL
                     PVYALTEGIQQWEMRKMIGRVVAQYSGYLEEVLPEKILAQQRLLPIQEAVQQIHAPSD
                     QQQIQAARRRFIYEELFILQVALALKRYQQITSKKSPILRLTPKIDERIRRLFPFRLT
                     AAQDQAVAEIVRDMEQPIPMNRLLQGDVGSGKTVVAVYAMLLAVANQHQAAMMAPTEI
                     LARQHFQTINQLLEKSRVRRALLVGGLPPQERAQILRQIA"
BASE COUNT         2824 a         3248 c         3422 g         2986 t
ORIGIN      
        1 tgacacgata tgtcaccgaa accgttaatg ttccgggata gttttcctcc ccgtcgggac
       61 taacgcggga gaattcgacc ccaacccagc cgtcaccctg cacaggccga tggtcccaga
      121 cccgctgatg gaatccgacc ttgccgccat ggagatgatt ggggccgttg tttgttgcca
      181 attggtaagt cacaccatcc aactgaaact gtcccttggc aattcggttt gcataacgcc
      241 cacatatgga accaaagcag ggatggccct tgaggtaatc ctcgagggac tctagataaa
      301 gggtgacgtt ctcgcgtttt ccctctcggt caggaaactc cacagccgtc aacgtggcgc
      361 ccaatgacat gacacgcaca accaaccccc ggccgttgtc caaagtgtac agaggtatga
      421 cttcgccact gggcatggtt ccaaaaactt gactctcgac tttcatagca cttccctcat
      481 ttgcattagc ggtaagaacc gcgccaaaag cgaacaccgt taccacagtg atcgaccata
      541 tattggagga ccagcctatc cgacgtaacc gctggggtgg caaaaccgtc gaccattgcc
      601 aaatattgga aatcaaagac atgactcaac ctcccttccc gtgctccggt ttaaatcccg
      661 cgctctgacg accaatgttg ggttgggcac caagtttggt tacaaggcta ccggtcatgc
      721 aaaacctgcg cgattcgcac tgaccaaacg tttggcctcc ctcgccagaa tataatcctc
      781 ttgactgcga ataactagaa tgcgaccggg agaattgtcg gaggaaatgt cagcatctgg
      841 ttggcacgca gcatttcgct cggggttcaa aaatagtcca agacactcca gcccgcggca
      901 gatgtcggcc cgcagttctg acgcattttc agcaattcca ccggtgaaaa cgagagcatc
      961 gacccttccc agcgttacgg ccaaagcgcc gagcattgcc cgcacgcgat aagcatacat
     1021 ttctaacgct agttgagacc gtttgtgacc ggcctgggca gcccgaagga cttcgcgata
     1081 atcgcccgaa atgcctgaca cgccgaggag gcccgactgt gtatgcaaaa tccgttcgac
     1141 ctggtcagca gtcaggccat ggtggcgttg aacataggtg atgatgcccg ggtccacggt
     1201 tcctgaacgc gtccccatca taaagccttc cagcggggta aaacccatag tgttggtaac
     1261 agccttaccc tccgcgatcg cagtggccga agaaccttgc ccaagatggc acgtgatgag
     1321 atggaccgac tccaccgggc ggccgagcat ttgggctgca cgctccatgg cgtaagcgtg
     1381 gctcgttcca tgatagccga aacggcgaat accccactcg gtgtaccatt cgtagggaac
     1441 aggataaagg taagccgccg gggggatgtg ggcaaagaaa gccgtgtcga aaattccggt
     1501 ttggaccgcc ccgggaaata gagactcggt gacctcgata gcctgaagcc cagccggaat
     1561 gtgaagcggc gcaagcggga tgaattggcg aataatctct ttaatctgct gggtgataat
     1621 gaccggttcg cggatcgcct cgcctccatg aatcaggcga tgcgcaacgg ccggaatctc
     1681 ttcaggtgaa gccacaaagt gcaactcctg gaggagaccc agggctaccg cgaccgcatc
     1741 acggtacttt ttagaggagg acgtacgctg cgccacgacg gtatcnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnngcaga tcggctgtat tccacgtcaa gccaaacgat
     1921 tctcccggaa ccgtccaaaa gatacaaaca gggtggctga tgagccgaaa attggcggaa
     1981 aaacccacca tcggtgtcaa ccaaaattgt gaaattcgca ccggattctt ggatgatctt
     2041 ttcggtttcc tcggattttg ggcccacatg gatgcccaga acggccaccc cttgatcgcg
     2101 atattttgac tcgatctctg actgcatgtc accgagcagg ttaaccgcat tcaaggcggc
     2161 gtagcgttca gccccactgg tccagaagac caaaaccgtc gctttttttt cgaaaagatc
     2221 gctgatttgc tgcgtgccac cgcttaactg cgatagttca ccatggggag ctgaatcacc
     2281 cacaaataga ttgcatttgg cccggtcaat ctccgccata accaccttgg ggatttctac
     2341 tggttgacta ctctcttcgg atcgtcctgg agcgacggat tcggcgacag gtgctgatgg
     2401 gttcatgggc tcaacaggga tctcgctggg agcacttccg gaaccctgct gggctgccgc
     2461 cacatttgtt tgagattctt gcggggcttg tttcttcgaa cagccggtga caactaggga
     2521 cccgactaac aaaacgacgg ccagtcgttg tccaaggatt tgcgcagttt gcatacctgg
     2581 cgactcctat cgttcgacaa atgaaagaga acaagcaccg ttgttacttt tcaattttga
     2641 tccccagttc acgcagttgt ttcggatcga cctcactagg agcacctgtc atcaaatcta
     2701 cagcccgctg cgtcttagga aaggcgatgc agtcccgaat cgagtccacc ccggcgaaga
     2761 tcgccaccaa ccgatccacg ccaagggcga tcccaccgtg cggcggtgcc ccgtattgca
     2821 atgcctccaa gagaaagccg aagcgatctt tggcctttgc ttcatcgata cctagcaact
     2881 gaaagacttc ctgctgtaac tcggagtcat gaatacggat ggttcccccg ccgacctcgg
     2941 tgccattgag caccaggtcg tacgcctggg cgcgacactt tcccggatct gttttcaaaa
     3001 gttcgcggtc ttgggggcgc ggggctgtaa acgggtggtg catggaggtc cagccgcctt
     3061 cttcttcgtc gatggcaaac atgggaaact caacgaccca ggataggtgc atcgcacgtg
     3121 gatcgtacaa cttcaattct gcaccaaacc gttttcgcaa accgtacaac gccttacagg
     3181 taatttcaaa atcgtcggcg acgaagagca acaggtcggt gggttgtgca cccatcttgg
     3241 cccgaattgc ctcaagctgt gatgccgtga aattcttggc caccgtggag ttaagcgtgc
     3301 cttccggttc gacgcggaac cacacgagtc cctttgcccc gaagtcctgc ttaacccatt
     3361 cggtcaactc gtcgatttgc ttgcgggaat aacgattggc gccaccgggc actgttattc
     3421 cccgcacacg tttactattt tctgccgctt ctcgaaagac cctaaaatcg cactccgcgg
     3481 cgatagccgt aatgtccgta agctccatgc caaatcgcaa atcgggggcg tcgtggccaa
     3541 agcgttccat ggcctcgtca taagtcattc tcggcagggg caacgtaagt tcttggccca
     3601 gaatctcgcg gaacaagcgg gccatcagac cctcgacaat acccatgaca tcgtccgctt
     3661 ggacgaatga catttccaaa tcgagctgag tgaactccgg ctgccgatct gcccgtaggt
     3721 cttcatcgcg aaaacaccgg gcaatttgca tataacggtc gtagcccgcg accattagaa
     3781 tctgtttgta aagctgagga gactgcggaa gtgcgtaaaa cgttcccttt tgaattcggc
     3841 tagggacaag gtagtcgcgg gctccctcgg gcgtgcttcg gcccaacatg ggcgtttcga
     3901 cttcgataaa tccatgctca tcaaaataat ctcgcatcac cttgatcatc cgatgccgca
     3961 aaagcaaaat ttgttgcatc tggggccggc ggagatcaat gtatcgatac cgcaggcgca
     4021 cctcttcccc aggcaaatcc ggagtcgacg gcaagaaagg atgaacgttc gcccggttga
     4081 ggatacaaca ctgttctgcc cgaacctcga tttctcccgt ggccaattta gggttttccg
     4141 tgccctcggg gcggcgttcc acgagacccg ttacctgaat cacatactcc ggacgaagcg
     4201 agcggccaat ttggtgcaat tcttgaccat tgtcggagga aaagacaatc tgcgtctttc
     4261 cgtaacggtc tcgaagatcg acaaagacta cgcccttatg atcgcgatat gtatcaaccc
     4321 aaccacagag ggtcacatgt tgcccaatat gggaggttcg caactcgcca caggtatgcg
     4381 ttcgtaacaa agcgctagct cctttctata caagaggcaa ccgaacacgc gacttttggt
     4441 gatttgccca aaacctctaa gtagcccaca tacgccagca gctacagtat catctttgcg
     4501 tttgggccta gtcgcgcact cactgggtgc gtttgaaatc gacgcaaaac ccctatttta
     4561 ggctctggag gggcgttttc ctagatgagt agtgtcgggg ctcaggccat ggcaggggtc
     4621 cgttctacgg ctacaaaccc taaaacctgc gcggcggttc agccacccag tatgtctctg
     4681 gttcagacaa gccagcctgc actataatag tgcacaatca gaaacgtcct aatctcgcgt
     4741 aggattattg gaaattcgga gatcaatgaa cggccttttg gggaagggat gatcccgctc
     4801 ttctttggcc acctttggta gaaagacgtg ctgggagaca gagaaaattg gcaagacttc
     4861 ttgcgaaatt tggcttcgca tcgttggcga aaatcgacct gtgaaaagtt aatagaggtc
     4921 agatatggct gggctgttac aagccgaggg tccggatcga gggaaaatct atccgatcga
     4981 gaagaactgt gtgattctcg gtcggcatcc gaactgcgat attgttttgg aagtcggagc
     5041 tgtcagtcgc cagcacgcgc gaatcgtcca aacggcgtcg ggctttttcc tggaggacct
     5101 taacagcaga aatgggacct tcctcaacga gcagcgcgtt cacggccgtc aaccattgaa
     5161 ggatggtgac cgcatcagaa tttgtgactt ggtcttttct ttttttgaag ctgcgccacc
     5221 aaaaaaagaa ggcagtgaga ccgaaataac tgacgccgca tgggttgacg atgaggaacg
     5281 gtttcgacgc gattcaacaa ttgtgtcacg attcgacgtg acggcaacag ggagccgagt
     5341 tgaggtcaat cccgaagcga agctccgtgc gctgatggag ataagtcaaa atctgggcaa
     5401 agcgctatcg gttgacgagg tcctcgacaa aatcttgacc agccttttca atgtcttcac
     5461 ccaggctgac cggggattta tcatcctggt tgatcctcag acgggtaagt tggtgccgcg
     5521 ggccgtcaag actcgtcggc ccgaggaccg tgaacaactc cggatcagtc gcacgatcgt
     5581 caatgcggtg acctcgacaa aacaagccat cctttcagcc gatgtggcaa ccgactcccg
     5641 tttcgatttg agtcaaagca ttgtcgattt cccaatccat tccgtcatgt gtgtcccttt
     5701 ggtggggagc gaccgaaagg tactcggcgt catccaaatg gacacgcggc agcgcaaggc
     5761 ccgctttact ccggaagact tggaggtcct tgtcagcgcg gcgagtcagg ttgccgtttt
     5821 tgtggaaaac gcgattctgc atgacgcctc catgcgggaa gaactattgc ggcgcgaatt
     5881 ggctttggcg cacaaggttc aacagggttt tttgccatcg gaaccgccgc acgtgccgga
     5941 atacgatttt tttgattttt acgacccggc gcacgaagtg ggtggagact attacgatta
     6001 catccccctg cccgcaaacc gtttggcctt gatcatggct gatgtatcgg gcaaaggaat
     6061 cgctgctgcc ctccttatgg caaagctttc ggctgagatc aagtattgcc tggcgagcga
     6121 ggcgtcaccg cagaccgccc tgagacgcct taacaaagtg ttttgcagcg accgttggga
     6181 tgatcgtttt atcactttga ttctcgtggt tttggaccct gtgacccacc attattggct
     6241 ggttaacgcc ggtcattggc ctgcccttct caggaaggcg aacggcgaga ttctcgttgt
     6301 gggtgcagaa aaggcgggcc ctcctctcgg ggtctcactc gatgcaagtt atgacttcca
     6361 tgtagggacc ttcgatccag gggatctttt aatcttgttt accgatggtg tcgtggatgc
     6421 tatgaatggg aacggtgagg cctttggcaa cgaacgcttg atgaatgtat tacaacgtgc
     6481 atcaggttcg ccggaagagg tcggtaatag cctggtccaa gcagtccatc gctttgttgg
     6541 tcagcaaccg caagctgacg atatgtgcct agtcattgtg ggccgacaag ccaaggaccg
     6601 cgatgacaaa attgtggcta acaatgtttc ataacatcca caggtgtcag aaaggttttc
     6661 cagcccttaa aagccgtcat cgctacggaa gtaccaaaac ttcaaaagcc tacgctcgta
     6721 ttcggcccac gtgtcgcgga agacgtaaat aagaggttaa ccaacaataa ttccagacgt
     6781 tggatcgaag gtgggcaaac aggtgaaaag gtcgatccgg gcggcatcat agatgtcgct
     6841 ttccatacca gcggcaatca acttctctcc cgcggcgctg ttgcgaagct gagcggcaag
     6901 catttctggc gggatgggtt cggcacccga ggaatagggc ggtgcgaagg cggctcgcca
     6961 gttttcccgc gcagtaatcg cctgggcgtt gagctcatat cgcaggccac tcttccgttc
     7021 caagtggtcc accaacatcc ctgcaaataa ggtgtcgtcg cgagtgaatt ccccttcaat
     7081 accggaacat aggagatgca cggcatggct ttcttcaaac agggccttca gaacggccga
     7141 ggcattcaca aaagctccca ccagtactcg ttgggcccgg cgacacactt gaaatgcctg
     7201 agtaccattc gttgtcgtca gaatgataac ggcccgaaac acgacctccg ggcggtattc
     7261 cgacggtgaa tttcccaggt cgaatccggg aatcggtaaa caatcgcgtt caccggcaag
     7321 taacacgctt gtcgtgtcat cggtttcatg gaggatctcg gcaagtcgtt gggcatcttc
     7381 caccgaggca catggcacga ctgccgtagc cccggcagcg atggcgtgaa cgattgtcgt
     7441 cgtcgcacga aggatatcga tgacaacaac cgtgcctgtc tctaattcct tggggtcggc
     7501 gagactggga acggcgtaaa cattgagcct tggtagcatt gtggggcctc aaaaccggaa
     7561 ggatccaaga cgtaaaaatg tgcaaaaggt ccgatataat acaaagttag gatggtgtac
     7621 aagattttca caatgcgaag gatttaccat acgaaaaagg cgtcactttg acaaggcgtc
     7681 gatgttggcc tccactgggt gggttatttg cgcctcgtag tgcgcaggtg aggattaaag
     7741 aggtcgaagt tcatacgggc aagacgtaaa agacgagcct tctttgggtt agtttccgcg
     7801 tgcatcagtt gtcctcacaa tctattgaca agcgagctgg gcgaatccgg cggatgtttg
     7861 cagagatcgc cccgcgttat gatttgctga atcatttgct ctctttcggg acagatgttt
     7921 attggcgcga ccggacggtg aagtcctcag aggtgctcgg aacgggtccc gtcctcgatg
     7981 tttgttgtgg cactggggat ctgaccctgg ctttttggcg gcataggaga aatagttttc
     8041 caatcgtggg cgttgacttc tgtcgagaaa tggtgcaact agcaaagcga aaggctgatc
     8101 ggcgtcacgc ccaaatccat ttcatcgaag cggatggcct acatttaccc tttccggatg
     8161 atacttttgc cgttgtggcg gttgcctttg gactccgtaa catggccgac accgggcgag
     8221 ggctttctga gatggttcga gtctgtcggc ccgggggaga ggtcatggtg ctggagttta
     8281 cgttaccgac gctcttccca atcaacgtaa tttatcgagc gtactttcgt ttcttgcttc
     8341 cgatcatcgg tcaggctttg gctcccaatc ggcaggaagc ctataactac cttccggcta
     8401 gcgtccatga gtttcctcaa ggggaagctt tattggctga gatggccaag gcgggactaa
     8461 aagacttggt gctaagaccg ttctcattcg ggattgctac gttgtacaaa ggatcaaaat
     8521 gaaaaggcag attgttgtcg caatcaccgg cgccagtggc gtgacttacg ccattcgtct
     8581 catcgaggta cttcttgcgg ctgcctgtga tgtgcacttg accattagtc gggcgggtcg
     8641 gacggtgctc aaggaggagc tggacctcac cgtcgatctg gataatttcc agccatcgtc
     8701 tttgcttctt gacagtcaag acagcgccga ggacaccaaa cttcggcttc ttcgtggaat
     8761 ggctggggta gcaggagaat cgagtcacgt cttgggggtg cccatcggtg agcctggaac
     8821 gctttattac catcatttca aacggctgac ggcccccatt gccagcgggt catttatcac
     8881 ccatgggatg gtgatttgtc cgtgttcgat gagtacgcta agcaccattg ttcacggtgg
     8941 tggtaataac cttatccacc gagcggcgga ggtccatctc aaggagggaa gaaaactcat
     9001 cctggtgccc cgcgaaacac cattgtcttt gattcatctt cgaaacttgg cattggcggc
     9061 tgaagcggga gccacagttc tcccggctat gccggccttt tatcacaatg tgttaacggt
     9121 ccgtgaccta gtggacttca tcgtagcccg tattatcgat caactcggta ttgaaaacga
     9181 attgatccgt cgttggggca cataactcag agtttttgcg tatgagagat aatgcgtctt
     9241 tctcaaaaga cgttgcgatg accaatttgg gaggcgacca gttgggcgaa ggtctcggat
     9301 gtaatttagg aacagcgttt tcgccaatga cttggttgag gaaggtgcgt cattttcttg
     9361 agttgatcag attcagccac actctatttg ccctgccctt tgcagccttg tcagcggcga
     9421 tggtgtggaa ggtagtgcct tttcaatggc atcacctctt aggtttttta ctttgtatgg
     9481 tctttgctcg tagtaccgca atggcgttca atcgcctggt cgatcgaaag ttggatgcag
     9541 ccaatccccg tacggcgggc cgacatattc cgcgcggagt gctgggagtt cgtgaggttt
     9601 ggggtttcac tgccgtctgt gccattgggt ttgtcttatc gacgttgctc ttcttaccga
     9661 attattggcc gttggtagct tctggtcctg tgctggcttt tctttgtggt tacagctaca
     9721 gcaaacggtt tacgtggtta tcgcatttct ggctggggac ggcgttggct ttggcaccgc
     9781 cgggtatctg gttggccatc caaggtactt tagcttggcc gccgatcctc ctcgcttgtg
     9841 ccgtgatctt ttgggtggct gggttcgata ttatctacgc ctgccaagac tacgactttg
     9901 atcgtcgcca gaaactgtgg agcattcccg ctgtctttgg aataaggcga gccctatggt
     9961 tggcggcggc ttctcatggt gtgatggtgg gatgcttggc aatgctgcct attctgttta
    10021 acgaatttga ttggctttac tgggtgggac ttgcattggt ttctgtgctt ttgatctacg
    10081 agcattggct cgtccggccg gacgatctgc gacgagttaa cgaggcattt tttcatgtca
    10141 atgcggtggt gagcgttggt ttgttgatca taggaactgt tgacatttgg cttctgtaaa
    10201 aacctctggg aatcataatc ctcatgctag accagaacat cgtgcgaaca gagcgattag
    10261 ttgaatcgcg tctcgacgag attcgcaata agatcgaggc ggggcgtcgt ttgtcgtttg
    10321 aggacggggt ctttcttttc cagccctctg tgtccttgca cgttgtcgga cagttggccg
    10381 atcttgtttg ccgccgccgg catggtcgca ccgtctatta caacttaaac acccacctca
    10441 acccgacaaa tatctgcggt taccgatgca agttatgtgc cttctttagg tcaaaaggtg
    10501 accctgatgc ctacatcatg tcgcaggaag agattctaaa acgggcggag gatgccgatc
    10561 aggcaggctg taccgagttg catattgttg gtgggctacc cccgggcatc ccattcgagt
    10621 ggtaccttgg aattgttgag gcgatccata accagtaccc gcgacttcat ttgaaggcct
    10681 ggaccgccgt tgagattgcc tggtttgccc gaatcagcgg tctttcggtg aaggaagtcc
    10741 ttctccgtct aattgacgcc ggcttgggca gtctgcctgg tggtggggcc gagatcttcc
    10801 acccagaggt ccgcaggcag atttcctccc ataaagcaga tggcaagacc tggctcgacg
    10861 ttcatcggac cgcccatagc ctaggattga aaagcaatgc taccatgttg tttggacatg
    10921 tggaaacaat ctaccatcgt gtggatcatc tccttgaact gcggacactt caggacgaga
    10981 ccggggggtt tcaggcgttc gttgccttgc catttcatcc ggtgaggacg gccttttctc
    11041 atttgccgaa gctgtccgca ttcgactgtc ttcgaacaat cgcggtctcc cggctcttgc
    11101 tggataattt cgaccatatc aaagcgtact gggtagcctt ggggatcggt gtggcccaaa
    11161 ctgccttaga atatggggcc aacgatctgg acgggacagt gcgatatgag aatatttatc
    11221 atgatgccgg ggccgattct cccgatgtcc tatcggtcga ggagttgtgc gcgctcattc
    11281 gggagactgg acgcgagccg gtggagcgag acagccttta tcgccgtgtt cggcgcttcg
    11341 gacgggaatg ggaattggaa gacaatcacc ccgatgttcg taatcgagaa tgaatcgttc
    11401 tgtcggtgac acccctgctc ggaacattgc tgggttgtgc gaagcctgtc agaatcatga
    11461 tgaacgaggg aacaaagcca tctacgatct cgccaaggga tcaggatccc ctccggcttc
    11521 ccatccaatt tgtaaaaggg gtaggaccac ggcgggctga gatcctggcc aaactgggga
    11581 ttgaaaccgt cagcgatgcc ctcttctttt ttccgcgatc ttatgaggat tgcagcaggg
    11641 tacggccggt gaatcaattg gaagaaggaa ccgtccaggc agttgtgggt gttgtcgaag
    11701 acgtggagag ccgggtcctc cggggtggcc agactgtcgt gggtgtgctg atcgctggag
    11761 agggtggata cgtccgggct gtgtggtttc agcagcccta ccgccgggat tattttcgcg
    11821 aaggtcaacg tgtcatggcg gtgggaaaac cgcggcgcaa tgggttgatt tgggagatgg
    11881 ttcatccccg ggtcattgtt ttaggagagg aggaacaacc aaaactagcc cctcttcctg
    11941 tctatgcctt gaccgaggga atccaacaat gggaaatgcg caaaatgatt gggcgggtgg
    12001 tggcccaata tagcggatat ttggaagagg tgcttcccga gaaaatcctc gcccaacagc
    12061 gactcttgcc aattcaggag gcagttcagc agattcacgc ccccagtgat cagcagcaaa
    12121 tccaagccgc acggcgtcgg ttcatttacg aagagctatt catcctccaa gtggccttgg
    12181 ccttgaagcg gtatcagcag atcactagta aaaaatcgcc catccttcga ttgactccca
    12241 aaattgacga gagaattcgt cggctctttc cttttagatt gactgccgcc caagaccagg
    12301 ccgttgccga gattgttcga gacatggagc agccgattcc catgaatcgg ttgcttcaag
    12361 gggatgtggg aagtggaaaa acagtcgttg ctgtttatgc catgctcttg gccgtggcaa
    12421 atcagcatca ggctgcgatg atggcgccca ccgagattct tgcccggcag cattttcaga
    12481 ctattaacca gttgcttgag aaaagccgtg tccgacgggc attgctcgtc ggcgggctgc
    12541 cgccacaaga acgagcccag attcttcgac agatcgccga
//
LOCUS       DAOSUT010000123        12577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2935_length_12577_cov_9.622265, whole genome shotgun sequence.
ACCESSION   DAOSUT010000123 DAOSUT010000000
VERSION     DAOSUT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2935_length_12577_cov_9.622265"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLD05_11340"
     CDS             complement(<1..262)
                     /locus_tag="PLD05_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78076.1"
                     /translation="MNKHPTPRIRFFVDPELQTQLALRVIVYWGMCIATVVLILICWR
                     VVSGPVRPFYRHLGELWFHIGPALAASFVVLPIVVIDVIRVSN"
     gene            748..2676
                     /locus_tag="PLD05_11345"
     CDS             748..2676
                     /locus_tag="PLD05_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOP78077.1"
                     /translation="MSIVAKLPIPSHVWISRWKPILRRICELEPQLQSLPDGDLRKRS
                     LALRYRARSGEPLGHLLVEAYALVREAARRKLNMRHFDVQILGGIAMFHHAIVEMQTG
                     EGKTLTATLPMYLYALAGRGCHLATVNDYLARRDAEWMHPIYELLGLSVGVIQTQMSQ
                     AERRRAYAADVTYGTAKEFGFDFLRDRLLLRRIREGQTDLIGAMLEGNHAEMDEKPVQ
                     REPFFALVDEADSILIDEARTPLIISALPTEEERLAVECYKWAAQVAHEFVEDIDYEY
                     DHEKKTVELTREGRRKARALPKPEAMASVGMFHIYEYVERAIKVARDFIRDRDYVVKD
                     GEIVIVDEFTGRLAEGRKWRDGIHQAVEAKEGVEVTIATGQAARVTVQDFFLRYRHLA
                     GMTGTAKDSAWELRSIYKCPVIVIPTNRPVIRKRLPDLVFGTQEAKWQAVVDEVCEMH
                     AIGRPVLIGTRSIDKSEHLSRLLHERGIDHQVLNAHHIEEEAKIVAEAGQRGRVTVST
                     NMAGRGTDIRLGPGVAELGGLHVICTEIHDAARIDWQLRGRCGRQGDPGTFRQFLALD
                     DEILVKAFGAKKAEKYAAIGRSRPGLFNEYLPIFRKAQRIMEKRHFRERRALMHFERE
                     RKKMQRQMGQDPYLDTPG"
     gene            complement(2798..4093)
                     /locus_tag="PLD05_11350"
     CDS             complement(2798..4093)
                     /locus_tag="PLD05_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78078.1"
                     /translation="MKGLHVGWVTVLVCVVGLLGPLGCQKPSSRPETSKPGAQQQGEV
                     ASPSGKFTPSPPEVRCLKLGDEEAPSVIYVQGYPLVFTGQVFPEAEKSGAIPDALEDQ
                     LALAWKRLETILEKAGSKKGKIVRIGICIAQEDDASPARVFVRQQFSKDWLPAITTVV
                     TPLPYPKSRVAFDAVAYLEGQPEDVRLVRLSDDPNAPHDAAVCPQGGLVFFSGQPDKN
                     PLPGSVENAMKALFETAQNLHVEKKDILQIRVFLQPMSEAPAVLNELRKAFPEGAMPP
                     VIFVEWIASAPVEIEMIARLPAQADLGGDVVRFYNPPGVKPSATFSRVTLVRTDRFIF
                     FPALLSRKDGSGAEQVDDIFAQLIEGLKVVGSDIRHLLKATYYVVDKEASDAVDDVRK
                     KVYDPQRPPAASKVTIHGVGQAGRTVSVDMIAAPAVAAQ"
     gene            complement(4146..5576)
                     /locus_tag="PLD05_11355"
     CDS             complement(4146..5576)
                     /locus_tag="PLD05_11355"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78079.1"
                     /translation="MTRKSQKEYRLSRRDLLAKSAKSVVAVALASQFVGCPKQEGAPQ
                     IIPESAKGANERIGVGIIGCGRRNGQLVTGKGGQGKPPKEAQIVAVADVNLRRAQQWA
                     EKYKCPAFQDYRKLLEMKEVDVVIYATPEHSHYLPCIHACQAGKDIYGEQPLSHTIRE
                     GQVMIQAVRKYKRVFQVGEQQRSHPATRKAVELILNGRIGKVHTVIGYNYPSPWDAEF
                     PAEQVPPDLDWDHWCGQNEVIPFNINVYLSRIPYNRENYPAEPAEKSAVGPGWMSLRP
                     YSGGELVNWGCHGLSMVLWGLGMDNSGPVEIWVEPPKKLEKLVYTQPEDRKRGDAAMS
                     QGIIHYRFPNDVVLKLEGTDERLGGGGTFIGEKGKITIFRGGYECDPPDLDKDPLPAN
                     AVRVYESDNHMENFFECVKTRKDPITPVEVAHRIATLCHLGNIARWLGRPLKWDPEKE
                     IFPGDDEANQYLDCPKRKGYELPEEV"
     gene            complement(5685..7049)
                     /locus_tag="PLD05_11360"
     CDS             complement(5685..7049)
                     /locus_tag="PLD05_11360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP78080.1"
                     /translation="MSHFTTDRRAFLAKLGVLGASAWASGLAFGNVDVPKDEEKLAID
                     GGKPVREKMLAARPYGPQFYDDREKTELIEVLESRQPFRWWHGNSKVLQFEQAYAAHL
                     GVKYALGVTSGTTALVTAMAALEVGPGDEVILPAWTWYADYDAIVLAGALPVFAEVDE
                     SFNIDPNDIEAKITPQTKAVIVCHLQGTPADLEPIMAIAKKHGVRVLEDFAQCVGGKY
                     KGRYVGTMGDIGINSFQLSKTITSGEGGAVVTNDATLFERALRFHDVGSLRSPYTDLL
                     KGGLLAAFAGCNFRMSEFTGAVLKAQLEKLETICAKVRANARFVREGLSDLPGIKFRK
                     SPDIDGDIGVGVYLDLGNRARRDRFLRALRAEGVSAVGPVGSVILPTDKRIENKVTVH
                     PEWPSFRSQRGREIQYGAACCPRTIDIINRFGGVIIDPNFTEDELKDIVHAMRKVYLA
                     TRDV"
     gene            complement(7146..8489)
                     /locus_tag="PLD05_11365"
     CDS             complement(7146..8489)
                     /locus_tag="PLD05_11365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOP78081.1"
                     /translation="MVEKRSVTHRINRRAFVAGTSAALAMGMLESEAPSQTGGTSAVK
                     LAIEGGEKAVTRSPGSGMRWGDRELKQLQEMLQQSSLFYWNGPQTALLKKRFQEVCPL
                     KYVQTCSSGTAAIHIAVASAGIGLGDEVITSPITDIGTVIGIIYQQAVPVFADLGPGT
                     YNLDPTDVERRITPRTKAIIAVHLTGNPCDMDRLKDIAKRHNLVLIEDCCQAWGAKYR
                     GRPIGTIGDFACFSLQDSKHVTCGDGGIVATSDERFGPNIQRFGDKGYDRTRGLGLEV
                     FATNYRMSEPQAAVAAAQLTRLEEVASRRYQLGTLLGELLADVPGIIPHQVHPDDRPV
                     YWFYMFRIKPEALRCNRSQFTRALQAEGVQASGGYIAKPLYKEPVFQNHAFFAGRWPV
                     KEMGLTTMDFTKHRCPEAEAILETCIRVPIKEFMDEEYIRQVGAAIRKVARYYSA"
     assembly_gap    8900..8999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9000..10653
                     /locus_tag="PLD05_11370"
     CDS             <9000..10653
                     /locus_tag="PLD05_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOP78082.1"
                     /translation="PLRVGVVQMAVAESLEANRDRIVIGINQATKHGVRVVVFPEGAL
                     RSQENHNAAAVAEAIDVIRDTARHCNVYVVFGGYTYLPHLKKEANWMLVIGPDGRDVF
                     RYEKLYDNHHATMPGVFFIDGIPCSAIICADRWLRGVEEIPVQQGAQISFELSNNFAS
                     EWVAPFGWYWYVPRALRNNVWVVFANTGNKAPDTQDSPLPIRELKHGHSAIIAPDGSI
                     VAAAHDDAETIVVADIDHNQATRAEARARASNPVLAPFWQAGIRLQQGQVVEAPPLVP
                     LESAVVDITLAAAQVTGDYSDLEPMIAEARLKGADMVIFPERATTENNLERLKLAARQ
                     HRITVVVGMETQHQGARRNSAFVIGPDGSVLTRYDQIAAVSPFRPGDNPSAMWFRVKG
                     VPAVVTIGRDALWTELAELAAVAGAQIHVHLDNDPATDESTSLRRLQVWCNLASFQTF
                     TATVNVVGSAIWDDLRSAEERRAEVRGLPRPDTGAVEVYSPFSANLVVSAGTGKQLIL
                     ATRRVEARNRYHPSRTSNFNPQMDAWYRLGAATIGPKNLPSF"
     assembly_gap    10671..10770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10771..12576
                     /locus_tag="PLD05_11375"
     CDS             <10771..12576
                     /locus_tag="PLD05_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78083.1"
                     /translation="VTGDEECTPEKLRRLLDEHQRPVSTIARQQVRIEAENFRYLDGC
                     ALEDRNDKNASHALNTRLAGGTTGSIRTRFHEPFARDSASYDVEVRCLDTNNARARLA
                     LYVNGALQGTWQAPGEARGWTSHTIQDVKIRHGDELRVDVQTDNAEAGRLDYVQLNER
                     TAQFATSNPILPQVMAAEPLDNPRALPGQIIVAGGRPGYLKINGGPAIFLCGPDNPEE
                     FLFLGKLLPDGTRDGPQMEMIEFLGQTGVNAMHFQMFRMRRCNIKGEGDDTHCPFIDH
                     DPSKPLNEKVLDQWEFWLKELEKRNIIVHLEFYNDATDVELMGWTLDAQGNLHPDERR
                     FIEGIVNRFKHLKNIIWGLEESSNKLPRSRVVHFRKMAELIRQVDEYQHPIVQSLVTP
                     ETAEKDMHPDGVTSADYRDSPVIDIVTWLHIPPHGKDFEAQHRAYREYAQKDRDVFIL
                     MKNETEYHQIDRRTARVHNWACALAGMHALEAQLNVARADRHDRIVDAGKLVSFMEQT
                     DWFRMKSADDMVTEDAKWALANPGVSYIVYSYDCKNVMGLKNLPQGRYEILWFDTIDG
                     KFQKETFQQAKDGPASWHKPANFSNEVAVYVKRIP"
BASE COUNT         2868 a         3552 c         3270 g         2687 t
ORIGIN      
        1 tgttgctcac gcgaatcacg tcaatgacca cgatcgggag cacaacaaac gaggccgcca
       61 aggcagggcc gatgtggaac catagctcgc cgagatgccg ataaaaaggt cggaccggac
      121 cgctaacgac ccgccagcag atgaggatta gcaccaccgt ggcaatgcac atcccccaat
      181 agacgatcac ccggagtgct aactgcgtct gcaattccgg atcaacaaaa aaccgtatgc
      241 ggggcgtcgg gtgcttattc atagcagaac tttcttcgct tgtccctcta ggtgcgacgt
      301 tcatatccgc ggactttcat tgtcccccct tttaaggagc ctaccgcaaa aagtccccct
      361 ctccctctgg gagagggttg gggtgagggc aagcggacgt cggaatcgat tgaaaaaacc
      421 ggcagcgatt cgagccaccc tcacccccga cccctctccc gccgaacggg agaggggaga
      481 tcagggatag gctcttaatt aaatgtgccc caagtggcat aatatctctt ggaaaatata
      541 ggtcacgatg ataccgtcaa gcggctcgca gattagattg gcttatcgca agaaatgaaa
      601 accaccatct tgacggactg tggttccccg ccaaaaacag cccacctgcc cgatcgttcg
      661 tctggcctat aatcagatat caagctacat tccccttacg acacgcggtc aacgtaaaag
      721 tatgtaaaac gataataata gtgcactatg tctattgttg ccaaacttcc cattccttca
      781 cacgtctgga ttagtcgctg gaagcctatt cttaggcgga tttgtgaact tgagcctcaa
      841 ctccagtcgc ttcccgacgg cgatctccgc aaacgaagtt tggccctccg ctatcgggcc
      901 cgcagcggcg aaccactggg acatctcttg gttgaagcct acgcccttgt ccgcgaggca
      961 gcgcggcgga agctcaatat gcgccatttc gacgtccaaa tcctcggtgg tatcgccatg
     1021 ttccaccacg ccatcgtgga aatgcaaacg ggcgaaggca agacgctaac ggcaacacta
     1081 ccaatgtacc tctacgccct ggctggtcgc ggttgtcacc tggccactgt taatgactat
     1141 ttggcgaggc gcgacgccga gtggatgcac ccgatttacg agttactggg gctttctgtg
     1201 ggtgtcattc aaacacagat gtcgcaagca gagcgacgtc gggcatatgc cgcagatgtg
     1261 acttacggaa cggctaagga atttggcttc gactttttgc gcgatcgcct tctcttgcgc
     1321 cggatacggg aaggacaaac cgacctgatc ggtgccatgc tcgagggaaa ccacgccgag
     1381 atggatgaga aaccggtcca acgtgagccg tttttcgccc tcgtggatga agccgacagc
     1441 atcttgatcg acgaggcccg gaccccgctg attattagcg cgctacccac ggaggaagaa
     1501 aggctggcgg tggagtgcta caaatgggct gcgcaggtag cccacgaatt cgttgaagat
     1561 atcgactacg agtatgacca tgagaaaaaa acggtagaac taacccgcga gggccgtcgc
     1621 aaggcccgcg cgctccccaa acctgaggca atggcatcgg tggggatgtt tcacatttat
     1681 gagtatgtgg aacgggcaat taaggttgct cgggacttta tccgcgatcg cgactacgtg
     1741 gtcaaggatg gcgaaatcgt catcgttgac gaattcaccg gacgcctcgc agaagggaga
     1801 aaatggcggg acggaatcca ccaggccgtg gaggccaaag aaggcgttga ggtcaccatt
     1861 gctacggggc aggccgcccg tgttaccgtg caggatttct tcttgcgcta ccgccatttg
     1921 gcagggatga cgggtacagc caaggactct gcatgggaat tgcgaagtat ttacaagtgt
     1981 cccgtgatcg tcattccgac gaaccggccg gtgattcgca aacggcttcc cgatttggtc
     2041 tttggaaccc aagaggccaa atggcaagcg gtggttgatg aagtctgcga gatgcacgcg
     2101 atcgggcggc ctgtgctcat tggtacccgc tcgattgaca agtctgagca cctatccaga
     2161 ttgctccacg agcgaggcat tgatcaccag gtcttgaatg cccatcacat cgaggaagaa
     2221 gccaagatcg tagcggaagc aggtcaacgg gggcgcgtca cggtttccac caacatggca
     2281 gggcggggaa ccgatatccg tctgggcccc ggcgttgcgg aattgggtgg acttcacgtt
     2341 atctgcaccg agatccacga cgcggcgcgg atcgactggc agcttcgcgg tcgctgtggc
     2401 cgccaaggtg atcccggcac ctttcgccaa ttcctcgccc tcgacgacga aatcctcgtc
     2461 aaggcctttg gggccaaaaa ggccgaaaaa tacgccgcca ttggtaggag ccgcccaggg
     2521 ctattcaacg agtacttgcc catcttccgc aaggcacagc gaatcatgga aaaacgccac
     2581 ttccgcgagc ggcgggcact gatgcatttc gaacgcgaac gaaagaagat gcagcgccaa
     2641 atggggcagg atccctactt ggacacaccg ggttgagcaa atccttagcc tcagcctatg
     2701 ccccgcgtcg ttggcagccg agcaaaccca cagacgcatg tttccgtttt gcggagttta
     2761 ccgagtgtct ccaagcgtcc ttttccagaa gaactcttca ttgggcagcc actgccggtg
     2821 cagcaatcat atcgacactg accgtccgcc cggcctgacc aacaccgtgg attgttacct
     2881 tcgacgccgc agggggccgc tggggatcat aaaccttttt tcgcacgtca tcgacggcat
     2941 cactggcctc tttatcgacc acataatatg tggcctttag aaggtgacgg atgtcactcc
     3001 ccaccacttt taaaccctct atcaattggg cgaagatatc atccacttgt tctgcaccag
     3061 aaccatcttt tcgcgacaac aacgccggaa agaagataaa ccggtcggtg cggaccaagg
     3121 taacccggct gaaggttgcc gacggtttga cacccggcgg attgtagaaa cgaacgacat
     3181 caccacccag atcggcttgg gccggtaatc gagcaatcat ttcgatttcc acaggtgcag
     3241 aggctatcca ttccacgaag atcaccgggg gcatggcacc ttcgggaaag gcctttcgca
     3301 actcgttgag cacagcaggg gcctcactca tcggttgaag gaagacacgg atttggagga
     3361 tgtctttctt ctccacgtga aggttctgag ccgtctcaaa taaggccttc atggcgtttt
     3421 ccactgaccc cggcaggggg tttttgtcag gttgcccaga gaagaacacc aaccctccct
     3481 gcggacacac agcagcatcg tgtggtgcgt tcggatcatc tgatagccgg accaacctca
     3541 catcctccgg ttgaccttcc aagtaagcca cggcatcaaa ggctacacgc gatttggggt
     3601 agggtagggg agtcaccacc gtagtgatgg ccggcagcca atccttcgaa aactgctgtc
     3661 gaacaaacac acgcgccggc gaagcatcat cttcctgcgc aatgcagata ccaatacgga
     3721 cgatcttccc ctttttcgag cccgctttct cgagaatggt ctcaagtcgc ttccatgcta
     3781 atgccagttg atcctccaag gcatcaggga tcgcaccgct cttctcggcc tcgggaaaga
     3841 cctgccctgt aaagacaaga ggatatcctt gcacatagat tacagacggg gcctcctcat
     3901 cacccaactt caagcatcgt acctctggtg ggctgggggt aaacttgccg gacggtgacg
     3961 cgacctcacc ctgttgctgc gcgcccggct tactggtctc ggggcgcgaa gacggtttct
     4021 ggcatcccag cggccccagc aaacccacaa cacaaaccaa aaccgtaacc catcccacat
     4081 gcaggccttt cataccggga tccccttagg cagttctgta gtgtgaatta tgccgagctg
     4141 agtcgtcaaa cctcttccgg cagctcatag cccttccgtt tgggacaatc gaggtactgg
     4201 tttgcctcgt cgtcacccgg gaagatttcc ttttctgggt cccattttaa tggtcggccc
     4261 aaccaacggg cgatgtttcc gagatggcaa agagtagcaa tacggtgggc cacttccacg
     4321 ggcgtgatgg gatccttccg cgttttcaca cattcaaaga agttctccat gtggttatca
     4381 ctctcataga cccttacggc gttagcgggc aagggatctt tatcgaggtc gggcgggtcg
     4441 cactcatagc caccacggaa gatggtgatc ttccctttct ccccaataaa cgtcccacca
     4501 ccacccaaac gctcatcggt gccttccaat ttgagcacaa catcattggg gaagcggtaa
     4561 tgaataatcc cctgactcat ggcggcgtcg ccgcgctttc gatcctcggg ctgagtatac
     4621 acaagctttt ccaacttctt aggaggttca acccaaatct ccaccgggcc cgagttatcc
     4681 atacccaagc cccaaaggac catactcaag ccgtgacaac cccagttcac gagctccccg
     4741 cccgaataag gacgaagcga catccagccc ggtcccacgg cactcttttc cgccggttct
     4801 gcgggatagt tctcccggtt gtatgggatt cgcgacagat acacattgat gttaaacggt
     4861 atcacctcgt tttggccgca ccaatgatcc caatcaagat ctggtggcac ctgttcagcc
     4921 ggaaattctg cgtcccatgg gcttggataa ttataaccaa tcaccgtgtg caccttgccg
     4981 atgcgaccgt tcaaaatcag ttcgacggcc tttcgcgtag cgggatgcga acgttgctgc
     5041 tcaccgacct gaaagactcg cttatacttt cgcaccgctt ggatcatcac ctgaccttct
     5101 cggatggtgt gactgagcgg ttgctcgccg tagatatcct tgccggcctg acacgcatgg
     5161 atacacggca aatagtggga atgctccggc gtcgcgtaaa tgacaacatc gacttccttc
     5221 atctccagga gttttctgta gtcctgaaac gcgggacatt tgtatttttc agcccattgt
     5281 tgtgcccgtc ttaaattgac atctgccacc gcgacaattt gagcttcctt ggggggtttc
     5341 ccctgtcccc ccttaccggt caccaattgt ccatttcttc gtccacaccc gataatgccc
     5401 acgccaatcc gttcatttgc tcccttggcg gattccggga tgatctgcgg tgcaccctct
     5461 tgcttgggac accccacaaa ttgcgaggct aacgctacgg cgacgaccga ctttgccgac
     5521 tttgccaaca aatcccggcg cgataaacga tattctttct gagatttcct agtcatggca
     5581 gagctccact atcatgccag cggatacaag cacaaccctc gtttccccca actttatctg
     5641 ctaaaacact gcacgagaaa taatcaggat cacctctgat accttcagac atcgcgcgtc
     5701 gccagataca ccttgcgcat cgcgtgaaca atgtctttta gctcatcctc ggtgaaattc
     5761 gggtctatga tgacgccacc aaagcgattg atgatgtcaa tcgttctcgg gcaacatgcg
     5821 gccccgtatt ggatttcgcg tcctcgctgg cttcggaagg agggccactc gggatggacg
     5881 gtaaccttgt tttcaattcg cttgtccgtc ggcaggatga ccgatcccac cggtcccacc
     5941 gctgaaactc cttccgcacg caaggcacgc aaaaaccgat cgcgtcgcgc ccgattaccc
     6001 aagtcgaggt acactccaac accgatatcg ccatcgatat ccggggactt gcggaactta
     6061 attcccggca gatcgctcag gccttcgcgg acaaaacgtg catttgcccg aaccttcgcg
     6121 caaatagtct caagcttttc caactgggcc ttaagcaccg ccccggtaaa ctcgctcata
     6181 cggaaattgc aaccagcaaa ggctgccaat agtcccccct taagcagatc ggtgtatggt
     6241 gaacgcaacg aacctacatc gtggaaccgc aaagcccttt caaacagcgt cgcatcgttt
     6301 gtcacgacgg caccgccctc gccagaggta atcgtcttgc taagttgaaa actgttgatg
     6361 ccgatatcgc ccatcgtgcc cacatatcgc cccttgtatt ttccaccgac acactgggcg
     6421 aagtcttcga gaacacgaac cccgtgcttc ttggcaatcg ccatgatcgg ctccagatcg
     6481 gccggtgtcc cttggaggtg gcagacaatg acggcctttg tctgcggagt gatcttagcc
     6541 tcgatgtcgt ttgggtcaat attgaacgac tcatccactt ccgcaaatac cggaagtgcc
     6601 ccagccagga cgatcgcatc gtaatcggca taccaggtcc atgcgggcaa aatgacctcg
     6661 tcacccggtc ccacttcgag cgcggccatg gcggtgacaa gcgccgttgt cccggaggtc
     6721 actcccaaag catacttgac acccaagtga gcggcatagg cctgttcaaa ctgcagaacc
     6781 tttgaattcc catgccacca ccggaacggc tgtcgcgatt caagaacttc tatgagttcc
     6841 gtcttttctc gatcgtcata gaattgcgga ccgtatggtc gtgcggccaa catcttttcc
     6901 cgcacgggtt ttccgccgtc gatggccagc ttttcctcat ccttgggtac atccacattc
     6961 ccgaatgcca acccgctggc ccacgcagac gcccccaaaa ctccgagttt cgccaaaaaa
     7021 gcgcgacggt ccgttgtaaa gtgagacatc gccgtaccct cccaaaaaat gcacctgaac
     7081 ccttgtagga tttgcgggac tctcccaaac acgggttcag agccgaggac tacgaaacgt
     7141 ttctattatg ccgagtaata cctcgcaacc tttcgaatgg ctgctcccac ctgccgaatg
     7201 tactcttcat ccatgaactc cttgatcggt acccgaatgc acgtctcaag gatggcctcc
     7261 gcttcgggac accgatgttt tgtgaaatcc atcgtcgtca gacccatttc cttaacaggc
     7321 cagcgtccgg cgaagaacgc atggttttgg aatacgggtt ctttgtaaag cggcttcgca
     7381 atatatccgc ccgatgcctg cactccttct gcctgcaacg cccgcgtaaa ctgagaccga
     7441 ttgcaacgaa gggcttccgg tttgatgcgg aacatgtaaa accaatacac gggtcgatca
     7501 tccggatgga cctggtgggg aatgattcct ggcacatcag ccagcaactc gcccaaaagg
     7561 gtccccagtt gatagcgccg cgaagcgacc tcttccaagc gcgttagttg agcggctgcc
     7621 acagcggcct ggggctcact catgcgataa ttggtcgcaa acacttctaa cccgagtccc
     7681 cgcgtccgat catatccctt gtcaccaaac cgttggatat tgggcccaaa tcgttcgtca
     7741 cttgtggcga caatcccccc atcgccgcag gtcacgtgtt tactgtcctg tagcgagaag
     7801 cacgcgaaat caccgatggt tccgatcggc cgaccacggt atttggcccc ccaagcttgg
     7861 caacagtcct cgatcagaac cagattatgt cgcttggcaa tgtctttaag ccgatccata
     7921 tcacaagggt taccggtcaa gtgaaccgcg atgatcgcct tggtacgagg cgtaattcgc
     7981 cgctccacat ccgttggatc gagattatat gtccccgggc caaggtcggc gaaaaccggc
     8041 acagcttgct gatagatgat cccgatcaca gttccgatgt cagtgatggg agatgtgatc
     8101 acttcatcac cgaggccaat tccagccgat gccacggcaa tgtggatggc tgccgtgccc
     8161 gaagaacagg tctggacata cttcagcgga caaacctctt ggaaccgctt cttgagaagg
     8221 gccgtttggg ggccattcca gtagaataag gaactctgct gaagcatctc ttgcagttgc
     8281 ttgagttcgc gatcacccca acgcatcccc gaaccaggtg agcgcgtcac ggccttctcg
     8341 ccgccttcaa tagccaactt taccgccgag gtgcctcccg tctggcttgg tgcttccgat
     8401 tccagcatcc ccatcgccaa cgcagcagaa gttccggcga caaacgcacg gcggttaatc
     8461 cggtgggtta cactgcgttt ttccaccatg gtcttaccct cccacatcac tgccgattgg
     8521 ctttccgcct ggtgctccga tacgctaaaa agggcaccat aaaaaacacc cggggcgcaa
     8581 aagccaacca caccgcgacc gaagtcccca gcaatcgcta tttcatgttt ccgctgctca
     8641 agttacgtca cgggataccg gttgtcaata ggctgaggtc gttcgccaaa acaactttga
     8701 gaagcgagtc agtcgcgtac ctgggcattt ttataatggc cacgaaccga agggtctgac
     8761 ggtttaggcg agataacacg aaggctggct accgcgtggt caattgacgc tcatcaaacc
     8821 cgcggttaga atgcgctggg ccattggttt tagccccgcc accacgatag aaatgatgcc
     8881 tttcccgggt gaggtttccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     9001 ccattgaggg tcggcgtcgt gcaaatggca gtggccgaga gccttgaagc gaatcgtgat
     9061 cgcattgtca tcggtattaa ccaggccaca aaacacggag ttcgtgtggt tgtttttccg
     9121 gaaggggcat tgcggagcca agaaaatcac aatgcggccg ctgtggcgga ggccatcgac
     9181 gttattagag acactgccag gcactgcaac gtctatgttg tgtttggcgg atacacgtat
     9241 ttgccccacc taaaaaagga agccaactgg atgcttgtaa tcggtccaga tggacgtgat
     9301 gtcttccgct acgaaaaact ctacgacaat caccacgcca caatgccagg cgtatttttc
     9361 atcgatggca tcccctgtag tgcgatcatc tgcgccgacc ggtggcttcg tggtgtggag
     9421 gaaattcccg tccaacaagg tgcccagatt agctttgaat tgtcaaataa tttcgccagc
     9481 gaatgggtgg cgcctttcgg gtggtattgg tatgttccgc gggcgttacg aaataacgtc
     9541 tgggtagtct ttgccaatac gggcaataaa gcgccagata cccaggacag tcccctccct
     9601 atccgcgagt tgaagcacgg acatagtgcc attattgcgc ctgatggaag catcgttgca
     9661 gcggcccacg atgatgcgga aacaattgtc gtggccgaca tagatcacaa ccaagccacc
     9721 cgcgcagagg cgcgggcccg cgccagtaac cctgtgcttg caccattttg gcaggcaggg
     9781 ataaggcttc agcagggcca ggtggttgag gccccaccgc ttgtgccgct agaatctgcc
     9841 gtcgtggata tcacgttggc cgcggcacag gtgaccggcg actattctga tttagagccg
     9901 atgatcgccg aagcccgttt gaaaggtgcc gatatggtga tctttcccga gcgagcaacc
     9961 acggagaata atttagaacg ccttaagcta gccgctcgcc agcatcggat caccgtagtc
    10021 gtcggcatgg aaactcaaca ccagggagca cgtcgcaatt ctgcgtttgt catcgggccg
    10081 gatggctcgg tgctcactcg ttacgatcaa atagcggcag tcagtccgtt tcgaccgggc
    10141 gacaatccgt cggccatgtg gtttcgtgtg aagggtgtgc cagcggtagt cacgattggg
    10201 cgcgatgcgc tgtggactga gttggccgaa ctcgccgccg tggcgggtgc ccagatccat
    10261 gtacaccttg acaatgatcc agctacggac gaaagtacat cgctccgtcg gcttcaggtc
    10321 tggtgcaatt tagcatcctt tcagactttc acagccacgg ttaacgtggt gggcagcgcc
    10381 atttgggacg acttgcggag cgcagaggaa aggcgtgccg aggttcgcgg cttgccccgt
    10441 ccggacaccg gggcagtgga ggtgtattcg ccgttttcgg ccaaccttgt ggttagcgcc
    10501 gggaccggga agcagctcat cttggccaca cgacgcgtcg aggcacgtaa ccggtaccac
    10561 cccagccgta cctctaactt caatccccaa atggacgctt ggtatcgact tggtgctgct
    10621 actattggac caaagaattt gccgagcttc tagcactatc ggaggaggaa nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtaaccggcg acgaggaatg cactcctgaa
    10801 aagctaagac gacttctgga tgaacatcaa cggccggtat ccacgattgc tcggcagcag
    10861 gtgcgaatcg aggcggagaa ctttcgttat ttggacggct gcgcgcttga agatcgcaac
    10921 gacaaaaacg cctctcatgc actcaacacg cggctagcgg gcggaaccac tggatccatt
    10981 cgcacacgtt ttcatgagcc ctttgctcgg gattctgcca gctacgatgt ggaagtccga
    11041 tgcttggaca ccaataacgc gcgcgctagg ttggccttgt atgttaacgg tgcacttcag
    11101 gggacgtggc aagcacccgg tgaagcccgt ggttggacta gccacacgat ccaggatgtg
    11161 aagattcgcc acggtgacga actccgcgtg gatgtgcaga cggacaacgc cgaagccggc
    11221 aggcttgact atgtccagct taacgagcgg acggcacaat ttgccacatc gaacccgatc
    11281 ctcccgcagg tcatggcggc ggagccgttg gataaccctc gtgcacttcc gggccaaatc
    11341 atcgtggcag gggggcggcc cggttatctc aagatcaacg gggggcctgc gatttttctc
    11401 tgtgggcctg ataacccgga ggaattccta ttccttggca aattgcttcc cgacggcaca
    11461 agagacggtc cgcaaatgga aatgatcgag tttttaggac agacgggcgt gaacgcgatg
    11521 cattttcaga tgtttcgcat gaggcgatgc aacattaaag gcgagggtga tgacacgcac
    11581 tgtccgttta tcgatcatga cccttctaag cccctgaatg aaaaggtatt ggaccaatgg
    11641 gaattctggc tcaaggagct agagaaacgg aacattattg tccatcttga gttctacaac
    11701 gacgcaacag acgttgagct gatgggctgg acgctcgatg ctcagggcaa tctccatcct
    11761 gatgaaaggc gttttattga aggtattgtg aaccgcttta agcatttgaa gaacatcatc
    11821 tgggggttgg aggaaagctc caacaaactc ccgcgatctc gggtcgttca tttccgcaag
    11881 atggcggagc tcatccggca ggtggacgag taccaacacc ccatcgtgca gagtcttgtc
    11941 accccagaaa cggccgaaaa ggatatgcat ccggacggtg tcaccagcgc ggattatcgt
    12001 gatagtcccg tcattgatat cgtgacatgg ctgcacattc ctcctcatgg gaaggacttc
    12061 gaggcacagc accgagctta tcgtgagtat gcccagaagg atcgagatgt tttcatctta
    12121 atgaagaacg aaacggagta ccaccagatt gatcggcgaa cagcgcgagt ccacaactgg
    12181 gcgtgtgccc tggccggaat gcacgcattg gaggcgcagc tcaatgtagc gcgtgcggat
    12241 cggcacgacc gtatcgtcga tgccgggaaa cttgtatcct tcatggagca aaccgactgg
    12301 ttccgcatga agtccgcgga cgatatggta acggaggatg ccaaatgggc cttggcaaac
    12361 ccgggggttt cctacatcgt ttattcgtac gattgcaaaa acgtgatggg attgaagaac
    12421 ttgcctcaag ggcggtacga aatcctctgg ttcgacacca tcgacggcaa gtttcagaaa
    12481 gaaacgttcc agcaagccaa agatggtccg gcctcgtggc ataaaccggc caatttcagc
    12541 aacgaagtcg ccgtgtatgt aaagcggatc ccttagg
//
LOCUS       DAOSUT010000124        12439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2960_length_12439_cov_9.553618, whole genome shotgun sequence.
ACCESSION   DAOSUT010000124 DAOSUT010000000
VERSION     DAOSUT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_12439_cov_9.553618"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLD05_11380"
     CDS             complement(<1..1229)
                     /locus_tag="PLD05_11380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HOP78084.1"
                     /translation="MSRGGSSGAAGSKALLLCRCDVEFGDEIYVARGLSIGRAPDNTF
                     VIEDPAVSRHHCQVEWDGAGGLILRCVDPGAVLEYKGGRVRELRLEAGLRFRVGPAEF
                     ECRLGGLGAKAVPRWVGQCPYCQSTDLPAPAPNVQACPACRRQVLVFRPRFSSGVACV
                     AGEFGGVRLVRLVGEGGMSVVFEGRVVKTGEPVAVKLLHPHLLEDADALRRFQREVSE
                     LRNLRHPRLVRIVGQGKWLGYPALVTEWLPGGSLAQQIESLKRKGEVASFAQALVWLR
                     QACEGLRVIHEAGLVHRDIKPSNLLLSAEGAIKIADLGLARRLGGETRGLTATGMAIG
                     SPKYMAPEQWDRPGEVDGRADIYALGMTFYELLTGRIPAGAWRPASELNPTVPKTFDV
                     ILERMLAPAKEERFANV"
     gene            1575..3068
                     /locus_tag="PLD05_11385"
     CDS             1575..3068
                     /locus_tag="PLD05_11385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOP78085.1"
                     /translation="MALEKFQLLPEVRDFLAKKRFGGVIGGQEVFASNGETFHTRDPG
                     TGDVLAEVANMQPDDVDQAVQAAAKAFRQTNWPNLTAHERGAILHRWVDLVESKKRII
                     AQIESLDCGKIYRQAEADVQNFIDTMRYFIEMSLHIERRAVLAVPHYEAYSYRAPWGP
                     CGFIFPWNFPILLVGWGIAPALAAGNTVVIKPAEDTPLSTLFVAHLAKEAGIPDGVVN
                     VVPGLGEVTGAALARHPGLRRMSFTGSPEVGRLVGEACARNLVPVKLELGGKGAAVVF
                     DDVDVSATVEKLVQAVTFHCGQVCCTATRWIIQKTIYDRFVSEAVKRLQEIRIGHELE
                     PRVQMGPVVNEKQKKRILSYIERGRAEGAEVILEGGELELPELGGFFVKPALLAGSLK
                     NVAAQEEIFGPVAYLTSFSDEADAIEKANDTEYGLANSVWTADLQRARRVAEAMEAAN
                     SWINAHNLFPHGVPYAGIKKSGLGGGVLSEQTYFDYLRSTSVVRPVS"
     gene            3544..3726
                     /locus_tag="PLD05_11390"
     CDS             3544..3726
                     /locus_tag="PLD05_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78086.1"
                     /translation="MGKGLCIFGMVGAGLLLLVFGLDLALGMPFGRISMIMDLGFIVA
                     AAGLFAASFMTLREIP"
     gene            complement(3766..5337)
                     /gene="cobA"
                     /locus_tag="PLD05_11395"
     CDS             complement(3766..5337)
                     /gene="cobA"
                     /locus_tag="PLD05_11395"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOP78087.1"
                     /translation="MMSDKPRRKDNEFASEGRPPSQGHVWIVGAGPGDPRFLTLWAKE
                     CLAAAEVVVYDYLVDPRILDIVPPHAEKISLGHHSRGRCLEPAEINEILLTRARDGKH
                     VVRLQAGDPAIFGRFAEELEFLRSKGIRFTVIPGITAGLAVGALAEIPLTHAQLASGV
                     ALIAGNEHSDKPHRNLDYQTLARFPGTIVYYMGVRSAAVWSQELMRHGKPAETPVAIV
                     RRCGWPEQQIWTTTLGGVADTIQSQNIKPPAIIVVGPVASLVPPRSWFTSQPLFGKRI
                     LVTRPEDQAADLQSLLETWGACVIKHPVIELMPPETWEPVDRAIEHLNAFAWIVFSSA
                     NGVKFFVDRLWALAKDVRVLAATKIAAIGPGTATALEERYLKPELVPNEYRAEALAEE
                     LNRLPARGNILLIRASRGRQVLPEMLSAAGWQVSQVVAYRSVDVTSANPEVIEQLEEG
                     RIDWTTVTSSGIAQSLVRLFGDRLKNTKLASISPVTTKTLHDLGFSPCVEAREYTMAG
                     LVSAILDYESPNVER"
     gene            5588..7054
                     /locus_tag="PLD05_11400"
     CDS             5588..7054
                     /locus_tag="PLD05_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78088.1"
                     /translation="MASETPAQTAGNVPPEPSPAKRKRLKECFEYGNRIAAQENFDYA
                     ADVYTECVVGEPGNALYVQAFLTNLKKKYNNNKRGKGLGFLKLAPLKAALRKAIHAKD
                     WVNVFKTGAEALKINPWDTGVLTALSEACDQCGFHEAQLVYLKMALDANPKDVEMCRR
                     CAKTLAELGLYDQAIAMWHKVEQLRPNDTEASKAVADLAVRKTIEAGGYDETGESRRR
                     VEAAGGAVSTADALSRKDRLEMEIKRQPNNLTLYIELAELYVREDDYARAEQVMAKAY
                     EVTKDVSAKEKLEDIQLRRLRQAMADAEKQYRASPNENTKAQLVAAQKALWAKELEVY
                     RDRVERFPNNLSFRFELGVRYQLNEMYEEAIKQYQMAQSDPRRKGVCLLNLGQCFEKI
                     GQHRLALKHFEDAIQEISDRDAENKKRALYAAAVLAYRLKDYRRAEQHASALAAMDYS
                     YRNVGELLDRIAKRLEAERSGSTEEAVEVELPPGQDED"
     gene            7151..7444
                     /gene="rpsT"
                     /locus_tag="PLD05_11405"
     CDS             7151..7444
                     /gene="rpsT"
                     /locus_tag="PLD05_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOP78089.1"
                     /translation="MPNTKSAKKRLRQNIKRRAHNRSIKRSVRTQCRKVREAVAAGDL
                     AKAEEEFRKAYRLLDRAGAKRIIHPNAAARTKSRLASAMRKARQSTVGAQSGA"
     gene            7699..8625
                     /locus_tag="PLD05_11410"
     CDS             7699..8625
                     /locus_tag="PLD05_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78090.1"
                     /translation="MKKERRQELKENELAEILARWYAKIAPHSQKILIAVLVIVIVYL
                     GWAIWSRWAAAVENEAWQALHAAMESGAAADYETVAERFPRTNAGYWAQLLAADTHLQ
                     IGCRQILVNKADGAQELRKALDGYLAVRNVTISPLFQQRVLWGLGRAYEALSGTREGQ
                     GELDRAMTAYRELVQRWPDGPFSSIAKRRLESLEKSETREFYDALAAYEPVRPSVQPP
                     SGIIDFGPSSLPTPPVQPSSGDETGAAPASDTKPGEQVVQEKSETSPPSEPTVEMPLP
                     DVNAPISETDQGQGEEKASEPKADSGEDKLDS"
     gene            8785..9783
                     /locus_tag="PLD05_11415"
     CDS             8785..9783
                     /locus_tag="PLD05_11415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOP78091.1"
                     /translation="MVVDSVLGENPIELIVTPHEADWRLDAFLVYHFPAYSRSLLRQV
                     ITAGGVTVDDTGAKPAYRLKVGQKVVVKLPELPRQCPKPENIPVEVLYEDDWLAVINK
                     PPGMVVHPARGHWSGTLASAIQYLYGGKLSTLGGPNRPGIVHRLDADTSGAIVIAKHD
                     LAHGKLASQFAERTVDKTYLAIVSGVPDRDADWIDEPIGLHPRQREKMAIRRYDPEAR
                     HAQTLYRVVERFRGFALVSAHPKTGRTHQIRVHLAHIGCPVLCDRYYGGRASLTRGEI
                     RGDPADETVILDRQALHAWKIAFTHPGTNERLEVVAPLPADMAGVLEELRQYRQAT"
     gene            complement(9806..10582)
                     /locus_tag="PLD05_11420"
     CDS             complement(9806..10582)
                     /locus_tag="PLD05_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOP78092.1"
                     /translation="MSSTRRIWDRRESTDDSLDVLVVGAHPDDVEIGCGGFVHALARK
                     GLRVGIVDLTDGEPTPECPHPDQRLDEAIKAAETLGVQLRVTLDLPNRRLFDDFESRI
                     ALAKEIRRYRPRIVLGLGGQTPMASPDHQQAAQIIEAAVFYARLTKWDELFENLAPHT
                     VGVYLHYYLAMRYPILPTPNAVVVDIGECLSVKLEAIGRYASQFAHRPEILNRIKTYN
                     QAIGQSVGFQAGEFIGHPTGWGFRDLIGGILGEGPRGPVE"
     gene            complement(10688..11959)
                     /gene="cbiE"
                     /locus_tag="PLD05_11425"
     CDS             complement(10688..11959)
                     /gene="cbiE"
                     /locus_tag="PLD05_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOP78093.1"
                     /translation="MANNARKIYVVGIGEDGFAGLTDYARQLIQSADLVVGEPHCLEL
                     IGEIAGQRVPVSSDLDEVVKTITENRDKQIVILALGDPLFYGIARYLWDKIGKEAFEV
                     VPHVSIMQMAFARVKESWEDALLVNLAHTSLEDLFNQIRSAETVGLFTTEELPPNVVA
                     QQLLSTGLDYFTAYVCENLGAPNERVTFGELADITKHTYSPLNVLILVRKPDVPDRPL
                     SLFGKRFFGNPDEAFLQSKPKRGLLTPREIRCIVLAELDVGPSSIVWDVGAGSGAVSV
                     EAAQLAREGHVYAIEMDPEDVRLIQENARRFGVTNLTPVLGRAPEAWQGLPNPDCVFI
                     GGSGRQVVGLCEAAYSRLQPGGRIVLTMGSLENVVDVHAFLRRHTKCLNVLMVNLARG
                     FYQFDRVRFQSLAPTFVVSAIKVKENSPKAG"
BASE COUNT         2823 a         3422 c         3594 g         2600 t
ORIGIN      
        1 ttcacgttag caaaccgctc ttctttggcc ggggcgagca tccgctctaa gatcacgtcg
       61 aaagtcttgg ggaccgtcgg gtttaactcg gaggccggtc gccaggcccc cgccggtatt
      121 cgccccgtaa ggagctcgta gaacgtcatc cccaaggcat agatatcggc acgcccatcc
      181 acttccccag gccggtccca ctgctccggc gccatgtact ttggcgagcc aatcgccatc
      241 cccgtcgccg tcagaccacg ggtctcgcct cccaaacgcc tggccaaacc taaatcggca
      301 atcttgattg ccccctcggc cgagagtaaa agatttgaag gcttgatgtc acgatgtacc
      361 agccctgcct cgtggattac ccgcagtccc tcgcaggcct gacgcagcca aaccagcgcc
      421 tgggcaaagg aggccacctc ccccttccgc ttcagacttt cgatctgttg ggccaaactc
      481 cctccaggga gccattcggt gaccaaggcc ggatacccca gccatttgcc ctggccgaca
      541 atcctcacca accgcgggtg ccttaggtta cggagttcgc tcacctcgcg ctgaaagcga
      601 cgcagtgcgt ccgcatcttc cagcaaatga ggatggagga gcttgaccgc caccggctcc
      661 cctgtcttga cgacgcgccc ctcgaacacc accgacatcc cgccttctcc caccagtcgc
      721 accaagcgga ctccgccaaa ttccccggcc acgcaggcca ctcccgagga aaaccgcggg
      781 cgaaagacaa gcacctggcg acgacacgcc ggacaggcct ggacatttgg cgctggtgcg
      841 ggcagatcgg tgctttggca gtagggacac tggccaaccc accgaggcac cgcttttgcc
      901 cccagacctc caaggcgaca ctcgaactcg gctggtccaa cccgaaaccg cagccccgct
      961 tcgagccgca actctctcac tcggccaccc ttgtactcta gcactgcccc gggatcaaca
     1021 caacgcaaga tcaggccacc cgccccatcc cattccacct ggcagtggtg acgggacacc
     1081 gccgggtcct cgatcacaaa ggtgttgtct ggcgctcgac cgatcgacag cccccgggcc
     1141 acatagatct cgtccccaaa ttccacgtcg cagcgacaaa gtaagagcgc cttgctcccg
     1201 gcagcacctg atgaccctcc gcgactcatt tctgcctcct cgtggtggcc cgaccgcgcc
     1261 tctgaataga ttgaccccgg ccacaaggtc aaacctgcca acttgcccta attctaattg
     1321 gctgagcttt tgcatgtcaa caaactgcct ggaggagctc cttagatgcc gcattgcgtg
     1381 gcgacatttg catgatgact ttgcaaggcc ttcaatgatc ccacgcgggc caacgcaaag
     1441 gcgcaaaggt agccgcgtga atgactcccc gttcttctgg gcgtttgata tattagagac
     1501 cgaaacctaa ccggcgaatc ggcgtgtgat tcgggggact cctgggactc gcttagccac
     1561 gaaaggaaaa gacgatggct ctggaaaaat ttcaactgct cccagaggtg cgcgatttct
     1621 tggccaagaa gcgttttgga ggtgtgatcg gaggccaaga ggtttttgcc tccaacggcg
     1681 agacgttcca cacgcgggac cctgggacgg gagacgtctt ggccgaggtg gccaatatgc
     1741 aacctgatga cgttgaccaa gccgttcagg ctgcggccaa ggcctttcgc caaaccaatt
     1801 ggccaaacct aacggctcac gagcgagggg ctattctcca tcggtgggtg gatctggtgg
     1861 aaagcaagaa acggatcatt gcccaaatcg aatcactgga ttgtggtaag atctatcggc
     1921 aggctgaggc cgacgtccag aactttatcg acacgatgcg atatttcatt gaaatgtcgc
     1981 ttcacatcga gcgccgggct gttctggccg tgccgcatta cgaggcttat tcctaccgtg
     2041 cgccgtgggg accgtgtggg ttcatctttc cctggaattt tccaatcctc ctggtcggtt
     2101 gggggattgc cccggcgctg gcggctggaa atactgtggt catcaaaccc gccgaggata
     2161 cccctctttc cacattgttt gttgcgcact tagccaagga ggccggtatt cccgatggcg
     2221 tcgtcaacgt ggtgccgggt ttaggagaag taacgggcgc agcgttggcg cggcatcccg
     2281 ggctaaggcg aatgtcattc acaggctcgc cggaggtcgg acggctcgtg ggtgaagcct
     2341 gtgcccgaaa tttggttccc gttaaactcg agctgggagg taaaggggct gcggtggtct
     2401 ttgatgacgt cgatgtgtcg gccaccgttg aaaagctggt ccaggccgtg acgttccatt
     2461 gcggacaggt gtgctgcaca gcgacccgct ggatcattca gaaaaccatc tacgatcggt
     2521 ttgtgtccga ggccgtcaaa cgactacaag aaatccgcat cggccacgag ctagaacctc
     2581 gagttcagat gggaccggtg gttaacgaaa agcagaaaaa acggattttg tcgtacattg
     2641 aacggggaag agcggagggg gctgaagtca ttttggaagg cggggaattg gagttgccgg
     2701 agttgggtgg cttcttcgtc aagccggcac tcttggcagg ttcgctgaag aacgtagcgg
     2761 ctcaagagga gatttttggg cccgtcgctt atctgacatc gttctctgac gaggcagatg
     2821 ccatcgagaa agcgaacgat accgaatacg gcctggccaa cagcgtctgg accgccgatt
     2881 tgcagcgggc tcgtcgggtg gcagaggcca tggaggccgc caatagctgg atcaacgccc
     2941 acaacctctt cccgcacggt gtgccatacg ccggtattaa gaaaagcggg ctaggagggg
     3001 gggtactctc ggagcaaact tacttcgatt atctccgcag tacctccgtt gttcgcccgg
     3061 tcagttgatc tcaggtccag ccccagtccg tttaggccgt tgtgggtttc ttagccttgt
     3121 taaaatgtgc cgggtaatta aactagagaa tgattcgctg gatgcgcaat tgcccctttc
     3181 gtgaaagggg agcgatgggg gcgtcgggac ctgactctgg cggatcaaaa tgcgcggatt
     3241 atctcggttg gatgaggggt gggataaaac gctagaaagt cgtaattgcc gttgcactgg
     3301 tagtttgccg gtgccatact atggtgtttt ggtttgttgc ggccgtttgg cgtggggaaa
     3361 atggcttggt ccgccgaggt aaagctatcc ggtggaaggg aattgagtcg ttgcttccgc
     3421 ttgaccttgg gtgacacagc atcgcgacaa gagaggcgag aaagggtgag cggttcgtca
     3481 gagtcacctt atttctatcg gggtaacacg cagcagggta tggcaccttg aggatcgaca
     3541 tcaatgggta aaggcctctg catcttcggt atggttggtg cgggacttct cctgctcgtt
     3601 ttcggactcg acctggccct gggaatgccg tttgggcgca tcagcatgat catggacctt
     3661 ggctttatcg ttgcagcggc gggccttttt gcagcgagct ttatgacgtt gcgggagatc
     3721 ccctagctaa attcgcaggt cacctcctct tcgcttttct ccaaatcagc gctccacgtt
     3781 aggcgactcg taatcgagaa tcgctgaaac aagtccggcc atcgtatatt cgcgggcttc
     3841 aacacaagga gaaaacccca gatcgtgaag cgtttttgtg gtgacggggc taatgcttgc
     3901 caacttggtg ttcttgagcc gatcgccgaa caaacggacc agcgattggg cgattccgga
     3961 acttgtcacg gtcgtccagt cgatgcgacc ttcttctagt tgttcaatga cctccgggtt
     4021 agccgaggtc acatccacgc ttcggtaggc caccacttga gaaacctgcc agccggccgc
     4081 actgagcatt tctggcaata cctgtcttcc ccgacttgcc ctaataagaa ggatgttccc
     4141 ccgcgccggc aatcgattca gttcttccgc aagggcctcc gcgcgatact catttggaac
     4201 gagttcgggt ttgaggtaac gctcttcaag ggctgtggcc gtcccaggtc caatcgcagc
     4261 tattttggtc gcggccagga cacgcacatc tttggccaat gcccacagtc ggtctacaaa
     4321 gaatttgacg ccatttgcac tggaaaaaac gatccaggca aacgcattca aatgttcgat
     4381 ggctcgatca acgggctccc aggtttctgg cggcatcagt tcgatgacag ggtgtttgat
     4441 gacgcatgcg ccccaggtct ccaaaagact ttgcaaatca gccgcctggt cttccggccg
     4501 ggtaaccaga attcgttttc cgaacagcgg ctgagaagtg aaccatgacc gtggcggaac
     4561 cagtgatgcc accggtccga ccacaataat ggcgggtggc ttaatgttct gggactggat
     4621 cgtgtcagcc actccgccga gcgttgttgt ccatatttgt tgctcaggcc agccgcaccg
     4681 ccggacaatc gccaccggcg tttctgctgg cttcccgtgc cgcatgagtt cctgactcca
     4741 gacggctgcc gaacggacgc ccatatagta aacgatggtc cctggaaaac gggccagggt
     4801 ttgatagtct aagttgcgat ggggcttatc ggagtgttcg tttcccgcaa tcagggcgac
     4861 acccgaggcg agttgcgcgt gcgttaaagg aatttcggcc aaggccccga cggctaggcc
     4921 cgccgttatc ccgggaatga ctgtaaaacg aatccccttc gatctaagga actccagttc
     4981 ttcggcaaac cgcccgaaga tggccgggtc accggcttgg aggcggacca catgttttcc
     5041 gtcgcgggcg cgggtcaata gaatctcgtt gatttccgct ggttctaggc aacggccgcg
     5101 gctgtgatgc cccagggaaa tcttttcggc atgaggcgga acgatgtcga gaattctggg
     5161 atcgaccaag tagtcataga cgaccacttc cgccgcggcg aggcactctt ttgcccagag
     5221 tgtgaggaaa cgagggtctc cgggccctgc ccctacgatc caaacgtgtc cctgtgaggg
     5281 tgggcgacct tccgaggcga attcgttgtc tttacggcgc ggtttgtcgg acatcatggc
     5341 accacgtttt gacttgcgaa acttgtcggt ttattacaaa ggctgaatga tcacgcttga
     5401 cctggccatg ggcaaggagg ccttctcgtt ccgcgagtta cgggttttcg gcatttcggc
     5461 atgttggagt tgacgacggc tgaggttaaa ataatcatga taggtgaaac tgggtcaacg
     5521 ggtgaggatt cctgaaacct taccttgagc gatgttggtg ggtccttaat tacttgtcga
     5581 ggccgaaatg gcatccgaaa cccctgctca aacggctggt aacgtaccgc ccgaaccgag
     5641 tccggcaaag cgaaagcgct tgaaagagtg cttcgaatat ggcaaccgca ttgccgctca
     5701 ggaaaacttc gattacgcag ccgacgtgta caccgaatgc gttgtcggag aaccaggcaa
     5761 cgcgctttat gtgcaggcct tcttaacgaa tcttaagaag aagtacaaca ataacaagcg
     5821 cggaaaaggc ttgggctttt tgaagttggc accccttaag gcagccctgc gcaaggccat
     5881 tcacgctaag gattgggtca atgtcttcaa aacgggcgca gaagccctca aaatcaaccc
     5941 ttgggacacg ggcgtcctga cggccttgtc ggaggcgtgc gatcaatgcg gtttccacga
     6001 agcacagctt gtttatctca aaatggccct ggatgccaac cccaaggatg tggagatgtg
     6061 ccgccggtgt gccaagacac tggctgaatt gggattgtat gaccaggcca tcgccatgtg
     6121 gcataaagtc gagcaattga ggcccaatga cacggaagcc tccaaagctg tggccgactt
     6181 ggcagtgcgt aaaacgatcg aggccggtgg ctacgatgaa accggtgagt cgcggcgacg
     6241 tgtcgaagca gcgggcggcg cggtaagcac ggccgatgcc ctcagccgta aggatcggct
     6301 ggaaatggaa atcaagcggc agccaaacaa cctcaccttg tacattgagt tagccgaact
     6361 ctatgttcgc gaggacgact atgccagggc cgaacaggtc atggcgaagg cctacgaggt
     6421 gacgaaagat gttagtgcta aggaaaagct agaggacatc cagcttcgcc ggttgcgtca
     6481 ggcaatggcg gatgccgaga aacaataccg agcctcaccg aatgagaaca caaaggccca
     6541 attggtggcg gcgcaaaagg ccttgtgggc aaaagaattg gaagtctatc gcgaccgcgt
     6601 ggagcggttc cccaacaact tgtcattccg gtttgaactg ggcgtccggt atcaattgaa
     6661 cgaaatgtac gaggaggcaa ttaaacagta ccagatggcc cagagcgacc cccggaggaa
     6721 aggcgtctgc ttgctaaacc tcggccaatg cttcgaaaaa attggccagc atagattggc
     6781 actcaagcat tttgaggatg caatccaaga aatttctgac cgggatgccg aaaacaagaa
     6841 acgggcgcta tacgccgcag cagtcttggc gtatcgcttg aaggactatc gccgagcgga
     6901 gcagcacgcc agcgccttgg ccgctatgga ttactcttac cgtaacgtgg gtgaactttt
     6961 ggaccgaatt gccaagcgac tggaggcaga aaggtccggt tccacggagg aagccgttga
     7021 ggtagagctt ccccctggac aggatgagga ttagcctcca taatggcatt ttcggtagat
     7081 caaagggaca cccgtccgtc tgatgggtgt cgggctagat gaacgagttc tttgttgtca
     7141 cggttgagat atgccaaata ccaaaagcgc aaagaaacgg cttcgtcaga acattaagag
     7201 gcgtgcgcac aatcggtcca tcaagcggtc cgtgcggaca cagtgtcgga aggttcggga
     7261 agcggtggcc gccggggatt tagccaaggc cgaggaggag tttcgaaaag cttaccgtct
     7321 gctagatcgg gcgggagcca agcggatcat tcatcccaat gcggctgccc gaacgaaatc
     7381 gcgcctcgcc agtgctatgc gaaaagcccg ccaatccaca gttggggcac agagcggggc
     7441 ctaaggtcag ttgcgcaacg tcgactgagg tttgaaaccg gcgaaagccg gtttttttct
     7501 ttgacacgct ggcttgggac gtctcttcct gaagtggtcg atctactgtt tgtcgggggc
     7561 ttccgataaa atgagggttt ctggcgggaa aggcttgggt aacgataaat ccctgcgagc
     7621 gtgacgtggg gaaaggagtc cctcccgcag gaaaacctga ggtggtcact tcagcaggcc
     7681 cattgcagga atcagacaat gaaaaaagag cgacggcaag agctaaaaga aaacgaactg
     7741 gcggaaattt tggcacggtg gtatgcaaaa attgccccgc attcccaaaa aatcctgatc
     7801 gccgtgctcg tgattgtgat tgtttatctt gggtgggcga tttggagtcg ctgggcggcc
     7861 gccgtggaga acgaggcctg gcaagccttg cacgctgcca tggaaagtgg tgcggctgcc
     7921 gattatgaga cggtggccga gaggtttccc cgtacgaacg ctggttactg ggctcaactc
     7981 cttgcggcag atacccatct ccaaatcggt tgccgccaga ttctcgtcaa taaagccgac
     8041 ggggcacagg aactccgtaa ggccctggac ggttacctgg ctgtacggaa tgtcacaatt
     8101 tctcccttat tccaacagcg ggtattgtgg ggacttggcc gggcctacga ggccctatcc
     8161 ggaacaaggg agggccaggg agaattggat cgggccatga ctgcctatcg cgagctagtc
     8221 caacgttggc ctgacggtcc atttagcagc attgctaaac ggcgattaga gtcgttggaa
     8281 aagtcggaaa cacgcgagtt ttacgacgcc ttggcggcct atgagccggt gaggccaagc
     8341 gtgcaaccgc catcggggat catcgacttt ggcccaagca gtctccccac gccccctgtg
     8401 cagccgagta gcggtgacga gaccggagcg gcccccgcct cggatacaaa gccgggcgag
     8461 caggtggtcc aggagaagag cgaaacatcg ccaccatcgg agccgaccgt tgagatgccg
     8521 ctgccggatg tcaatgcgcc gatatccgaa accgaccagg gacagggcga ggaaaaggcc
     8581 tcggaaccga aagcggattc gggcgaagat aagttagact cttagggaga ggcgacaccg
     8641 taagttgtcc aatccgtcgc gacaaagctg ttatcggaag gcattgaacc gcggtatgat
     8701 ggcggcgtac tcgtcggagt tgggagaggg cctggctgcg aacccgcttg gggctgtcgt
     8761 aataacaaca ggttttacca tcgcatggtc gtggacagcg tattgggaga aaatccgatt
     8821 gaactgatag tcactcccca tgaagccgac tggcgtctgg atgcttttct tgtctatcac
     8881 tttccggcat atagccgtag cctccttaga caggtcatta cggcaggtgg ggttaccgtt
     8941 gacgatacgg gggccaaacc ggcgtatcgg ctgaaagtcg gccaaaaggt agtggtcaag
     9001 ctcccagagc ttcctcggca atgccccaaa ccggagaaca taccggtcga ggtgctttac
     9061 gaagacgatt ggttagccgt tatcaataaa ccgccgggga tggttgtcca tccggcgcgt
     9121 ggacactggt cgggaacgct ggcgagcgcc atccagtatc tctatggggg caagttaagt
     9181 actcttggtg gcccaaatcg accagggatc gtccatcgtc ttgacgccga taccagtggt
     9241 gccattgtca ttgctaagca tgatttggca cacggtaagc tcgccagtca atttgctgag
     9301 cgcactgtgg ataagaccta tcttgcgatc gtctccgggg ttcccgaccg tgatgccgat
     9361 tggattgacg agccgatagg cctccaccca cggcagcggg agaagatggc cattcggcgt
     9421 tatgaccccg aggctcgtca cgcccaaacg ctgtaccggg ttgtggaacg attccgaggt
     9481 ttcgctctcg tttctgccca tccgaagacg gggagaactc atcagattcg tgtccatctc
     9541 gcacatatcg gttgtcccgt actgtgtgat cgctactatg gtgggcgtgc ttctctaaca
     9601 cgtggagaaa tccgtggcga tccggcagat gagacagtga tcctggaccg acaggccctt
     9661 cacgcatgga aaatcgcctt cacgcatccg ggcacgaatg aacgtttaga agtcgttgcg
     9721 ccgcttcccg ccgatatggc tggagttctc gaggaattaa gacagtatcg gcaagcgaca
     9781 tgacattacg gacgaaggga actgactatt cgaccggacc ccgtggacct tcgcccaaga
     9841 tgcccccaat caaatcgcga aaaccccatc cggtcgggtg cccaatgaac tcccctgctt
     9901 gaaatcccac gctttggcca atcgcctggt tgtaagtctt tatgcgattt agtatttcgg
     9961 gacgatgggc gaattgtgaa gcataccggc cgattgcttc caacttcacg ctgaggcact
    10021 caccgatgtc cacaacgact gcattcggcg ttggtagaat gggataacgc atggcaagat
    10081 agtagtgcaa atagaccccg acagtatgtg gggcgagatt ctcgaatagt tcatcccatt
    10141 tcgtaagccg ggcatagaaa acggccgcct caatgatttg ggcggcttgc tggtgatctg
    10201 gggacgccat gggggtctgc ccaccaaggc cgaggacaat gcggggacgg tatctgcgga
    10261 tctcttttgc cagggctatg cgggattcaa agtcgtcgaa tagccgtcgg ttgggcaaat
    10321 caagggtgac gcggagctgg acgccaagag tctccgccgc cttgatcgcc tcatccaacc
    10381 gttgatcggg gtggggacat tcgggcgtcg gctctccatc cgtaaggtcc acaatcccta
    10441 cccgaagccc cttcctggcc agggcgtgaa cgaatccgcc gcaaccgatt tcaacatcat
    10501 ccgggtgggc accgacaacc agcacatcca gagaatcatc agttgattca cgtcggtccc
    10561 aaattcggcg tgtcgaactc atagatacct cgtcgaagtc aaacgacgaa atgacacaag
    10621 tgacagactc acgacgcaaa aaagatagaa gatcaccgtg cccgaatgcg aaatctcatc
    10681 cgaggcctta tcctgccttg ggcgagttct ccttcacttt gatcgcagaa acgacaaagg
    10741 ttggagcaag tgattggaag cgaacgcggt caaattgata gaatcctcgc gcgagattga
    10801 ccatgagcac attgaggcac ttcgtgtgtc gccgcaaaaa ggcgtggaca tcgaccacgt
    10861 tttccaggct tcccatcgtc aagacgattc gcccaccagg ttgcaaacgg ctgtaggcgg
    10921 cctcacacag gccgacgacc tgacgaccac ttcccccgat gaagacacaa tcgggattcg
    10981 gaaggccctg ccaggcctct ggagcgcgac ccaagaccgg cgtgagattg gtcaccccaa
    11041 atcgccgggc attttcttga atgagtctta cgtcctcggg atccatttcg atggcgtaaa
    11101 cgtgtccctc gcgggcaagc tgggcggctt ccacactgac ggcaccgcta cccgcgccga
    11161 cgtcccaaac gatgctcgac ggtcctacgt ctagctccgc caggacaatg cagcgaatct
    11221 ctcgcggagt caaaagcccc cgtttcggtt tcgactgcaa aaaggcctcg tctgggttgc
    11281 caaaaaatcg cttgccgaaa agacttagcg gtcgatccgg gacatcaggt tttcgcacca
    11341 ggatcaagac attgagcggg gagtacgtgt gtttagtaat gtcagccagc tccccgaaag
    11401 tgacccgttc gttcggcgct cccaggtttt cgcacacgta ggccgtaaag tagtccaagc
    11461 ccgttgacaa gagttgctgg gcgaccacgt tggggggcag ctcctctgtc gtaaaaagac
    11521 cgaccgtctc cgcgcttctg atctggttga acagatcttc cagcgaagta tgggcaaggt
    11581 tcacaagaag ggcgtcttcc cagctttctt tgacgcgggc aaacgccatt tgcataatgc
    11641 tcacatgagg gacgacctcg aaagcttcct ttccaatctt gtcccacagg taccgtgcga
    11701 tgccgtaaaa caaggggtct cccaaggcca gaatgacgat ctgtttgtcc cggttttccg
    11761 tgattgtctt aacaacctcg tcgaggtcgg aagagacagg gacgcgttgt ccagcgatct
    11821 cgccaataag ctccagacaa tgtggctcgc cgactacgag gtctgcggat tgaatgagct
    11881 gacgggcgta gtctgtcaaa ccagcaaaac cgtcttcccc aattcccaca acgtagattt
    11941 tacgagcatt gttggccatc gtaagtaaac actcccatgc tgcgattgga ggtgccgata
    12001 ttcctaatcc gtgattatgg taaagcctat cttaaacggt gagctaggga aagatggagc
    12061 gagtgccaaa cgctcctctc agaacaacgg ctgatggtct tggtgggaat taacattgtg
    12121 gagtgtcgca ggcttaggcc gacgtatctt accggaggtt ggcccaatct aaaaggaagc
    12181 gcatccctac ggtggcgatg gcccgacaac agcagcgacc gccgatgcct cagccgaact
    12241 atgactccgc ctatcgaacg ccttgacaag accacccaaa taggcaaccc acgttctgac
    12301 ggcgggtgaa ggcctaccgc ctcttagccg gcactggtga acgtagccgt gccacagaag
    12361 aagagaaaac ccgcatacac gtggagagcg ggagacggga ttcgaacccg cgacccccag
    12421 cttgggaagc tagcgctct
//
LOCUS       DAOSUT010000125        12377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_2970_length_12377_cov_9.806931, whole genome shotgun sequence.
ACCESSION   DAOSUT010000125 DAOSUT010000000
VERSION     DAOSUT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2970_length_12377_cov_9.806931"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            533..1615
                     /locus_tag="PLD05_11430"
     CDS             533..1615
                     /locus_tag="PLD05_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOP78094.1"
                     /translation="MSEIKTHPASGVKKCICLGLSWAMVIFSAAIWDGIALGQTPAVL
                     SSFHVVDVTKRGDLRPPVITGLYYDAARDILITAGDDHLIRIFKQPGDQQLAVLNGQG
                     WIYGLVLDPTGKYLAAVDDRGLLTVWQWETARQLYRLPLSTAALRTVAFHPGGNWLAV
                     GGFEPRVFIVETLTGRRVADLNADRDVRQVVFSSDGASVVAAGGSGKLRLWDLPSLRQ
                     RMEITVSSRRLHAVAFSPDGRILAAGGEEPVVSLWQVDCDPPSLIGRLPLSDGIVTSL
                     TFCENGQQLAVGTTRNIIHVFDLVTSKELAQLEGHTGTISCLTWTPERSLLSSGSFDT
                     TVRFWRLLPGTTVSERLPTAQDILRQ"
     gene            1685..5416
                     /locus_tag="PLD05_11435"
     CDS             1685..5416
                     /locus_tag="PLD05_11435"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOP78095.1"
                     /translation="MKGWKGRPQQAPSQKRRDRHPSRYRKCRLEQMEPRMLLSVSPIQ
                     LGMVYYEDATGTDESGDTFELTFQGGAPGTQLTRLVINTDKLGDGLSIGDCFFDTEPG
                     GLGAFGYGPFAVISASGIDNFRVSVSDGGTELVLEFTGFEAGDRFIFTIDVDEMGFLG
                     PNAVAEGNEFEGSRLTAYFVAPHYYAAQGEDIFIDAYDHKLSASGLVLPHDSYSPPLP
                     EPEPVRTAGAFLEVTQTPLPVAISGRVFEDFNLDLTLNGEDRPLAGVTLELWQWQGET
                     YIFTGRTTVTDADGSYRFDDLLPGTYRIVERQPEGFLSVGAKAGTVGSEVRGSVLTSD
                     IIVGISLLGGEESIENNFAEVRPAELSGHVYHDVNNNGLLESGETGIGGVTVIVIREG
                     DHFLTEQEFTVITQTDGSWSVVDLYPGKYRVQEIQPAGWLDGLDRAGSLGGSAVNPGD
                     QIVNIKVPAGSQGVNYDFGELLPASLAGLVYLDLNTNCQYDPSEPVLPGVTIKLLSPD
                     GTVLRTTVTDASGRYVFENLTPGTYALHEIQPEGYFDGCDRPGTAGGVKSPPDLITDI
                     HLASGTAATDYLFGEIPPASVAGLVFSDLNANDQLDAEDTPIAAVTIQLFAANGELVA
                     TTVTDAEGRYRFENLRPGYYTIVEVQPVGYLDGADFVGSAGGQRDGNDRITDIKLPPG
                     VHATDYNFAEILPATISGYVFQDGPTITYVWGQERPDPYLVRDGQFTPDDIPISGVEI
                     QLRDAVGQPIVGEDGRPLVTWTDERGYYEFRGLRPGVYTLLEIHPAGYEDGLDTPGTL
                     GGIAVNPNASLDPAILAALTIDPRNDAILQVTVTPGAVGTNYNFAEVVVTSVPPYVPP
                     TPPPPWSPPERPASPVVPPGTPPIHLQISPVMPESVALPRFGGSAGTVDYTWHLSVIN
                     GGVPRHNLGGSQRVLIREVSLFNPARWEGIPLDRGQWTIVDIVTGETAVLGNLGTEAA
                     LPVVGDFSGIGRVQLGIFYGGMFFIDLNGNGIWDDQDLWIQLGKDRDQPVAGDWDGDG
                     KWDVGIFGPAWPGDDRALRAEPGLPDAQNRLTAFRPRNLPPTPDQAPDDQRLLQQGRN
                     GPTRADLIDHVFMYGQPGDHAVVGDWNGDGIATVGVFRGGTWYLDEDGDGRWGSRDRV
                     AFFGQAGDLPVVGDWNGDGITDLGVYRDGRFYLDIDGNGQLTAADKVITLGQPGDRPF
                     AGDFNGDRRAEVGVYRSQNPTSGGSQTLREAAAPRGVAK"
     gene            6223..7020
                     /locus_tag="PLD05_11440"
     CDS             6223..7020
                     /locus_tag="PLD05_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOP78096.1"
                     /translation="MLVLTRRENERIVLPTLKTFIQVVRIAGSTVRLGIEAPANVPVL
                     REELLKENRAPEDTARKPQVPCLDSFPETLEHSHDAASAPATRLSQQEKLLDCLALAC
                     DLADTHLANGKVAEARETIQKALELLRRLGARSVVAASSPVRVSGQRVALVIEPTKSE
                     GSSVSQVLRKAGYSVISFASGCRAIDYLASHNRPDLIVINLQENRPCAADTIRMIRQN
                     PALRGVKVVALSDSRPAEAEISIGPQGVDYWFATQTCPERILRQIEA"
     gene            7111..7956
                     /locus_tag="PLD05_11445"
     CDS             7111..7956
                     /locus_tag="PLD05_11445"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOP78097.1"
                     /translation="MIERDTLFRLSDGSSLRGRSWEPEGNPRARIFGLHGVLEHSGRY
                     GRLATDLTQAGYALEMVDLRGHGRSDGPRAFIPSWEQLLVDLDEVHAQLRAEVRVPDF
                     LFGHSMGAALTILWVTSRRPNIKGMILSAPPVMTPVRVPMLVRIVGRWLVHRWPELRI
                     VRLKTAERYGRHAVSRDPQVLEDLKNDPLVCRGPFPLKTGLLLLELPQRLQEAARACD
                     APFLLLQGTGDKLSSYRGAELFFQLAPARDKVLRLYEGLYHEVLSEPERAQVLADVLS
                     WLSAH"
     gene            8300..8728
                     /locus_tag="PLD05_11450"
     CDS             8300..8728
                     /locus_tag="PLD05_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78098.1"
                     /translation="MKRLLALLLGIGLLVYVTGCPKPATKPEGGTGPAPSTTAPAGST
                     EEQPAEQPAEKPAEQPAEQPAEQPAAGPELPAPAAPAEVTMPAPPAEQPAQQPAEEPA
                     AQPAQPAEQPAPAAEQPAPQPEQKPEEKPAEQPAEQPAQQ"
     gene            9045..10406
                     /gene="mtaB"
                     /locus_tag="PLD05_11455"
     CDS             9045..10406
                     /gene="mtaB"
                     /locus_tag="PLD05_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOP78099.1"
                     /translation="MPTLRTVTLGCKVNQYETEYLRQGFLRLGYQDAENGEPADLCIF
                     NTCTVTAQADSECRKILRRLARENPHAEIIVMGCFATKAAEEIRSLPGVSEVLTDKRQ
                     MGDLLARRGLVDIPTGISGFAKRHRAYVKIQDGCVFPCSYCIIPRVRGRLWSRPLREI
                     LNEVAQLVDNGYREIVLTGIHLGLYGHGPDDTDLPHLAGLVEAIIDSRSGFRVRLSSL
                     EVNEVTDSLLDLMAASPERICPHLHIPLQSGSNRVLKRMRRRADVGYFMERCALVREK
                     LPAPALTTDVMVGFPGETDEDFEATCRVVETVGFAKVHIFRFSPREGTPAAEFPDQIP
                     EKTKKDRARTLDAVAEKTRRQYINSLFGRQLQVLWEDLASAPGDNSPQWLLGMSDRYI
                     PVRALASRGKQGELDGVTVEGFDGTFLLDRDLFAVTSPPEMCQNNAQVGCVGCNFDDF
                     PTS"
     gene            10594..11043
                     /locus_tag="PLD05_11460"
     CDS             10594..11043
                     /locus_tag="PLD05_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP78100.1"
                     /translation="MATKKNSSTSLASEEKQALDSPNAQAVETRRKRILIVDDDVEIV
                     ESIRVALESLGYQILIARDGNQGLALADAEDPDLVILDMMMPKRSGFLVLEKLRQTRK
                     VPVRVIMITANEGSRHKAYAEMLGVDDYIRKPFAMDRLIESVQRLLE"
     gene            complement(11114..11407)
                     /locus_tag="PLD05_11465"
     CDS             complement(11114..11407)
                     /locus_tag="PLD05_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78101.1"
                     /translation="MRLMILGGSGLVGVAQLSEAVEAAEWLNAELIKAALHTATPEEE
                     GFVEYVVGLVQQGQLPAQILQSAYLWAQKRPKYKFQYFKRAIIYLAAQKGIRI"
     gene            11684..12283
                     /locus_tag="PLD05_11470"
     CDS             11684..12283
                     /locus_tag="PLD05_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOP78102.1"
                     /translation="MCIVFHKKRAKVTYRNTDNSPQGERRGSGWLLAILALSLVLSAG
                     CVQRRLIIRSQPPGAMVYVDDYQIGPTPIATSFTYYGTRKIRLVKDGYQTLTVYERID
                     PPWYQIPPIDFFAENVLGREVRDTRVLEYQLQPQPISPTQQLLDRAETLRRQTGGSPP
                     LPAAAPIQQTQQPGMPSGAPPTEGSTAPAFIIEPPRTSY"
BASE COUNT         2609 a         3594 c         3563 g         2611 t
ORIGIN      
        1 ggtcgccgtc gccggcgacc aaatcatcgg acggcggcga ccccgtccct accgttcacg
       61 gaactattcc agaaagctca cgaagacccc ttctcccagc gcgaagtgat cggcgctggg
      121 cgaccgcgtc ccttccaccc taaaagtcta tccccaaaaa atccccctct cccgccggga
      181 gagggttggg gtgagggcaa gcgaacgtcg gaatcgatcg ggaaaaccgg cagtgattcg
      241 agccaccctc acccccgacc cctctcccgc cgaacgggcg tggggagatt acgggatagg
      301 ctctaaacgg ggcgtgccgg taaagtcacc gctaccgaca aagattagcc acttttcgcc
      361 gtttctcccg ttatcaaaac cgtaacacag cgcgagattt ccgcctgcgg accttgtttc
      421 gcagaccgtt gatgccaaga cccgataaaa gaacgtgggc gatcattgtc atcgccgtgc
      481 cataggccta caccaatggg cctacgctcg gctttttggc gagggaacaa tcatgtctga
      541 aataaagacc catccagctt ccggggtaaa gaagtgcatc tgtctcggtc tatcttgggc
      601 gatggtgatt ttctccgcgg cgatctggga tggcatcgcg cttgggcaga ccccggccgt
      661 cctaagttca ttccatgtcg tggatgtgac gaagcgaggg gatctgcgtc cgcctgtaat
      721 cactggactc tattacgacg ccgcacggga cattctgatc actgctggcg atgaccacct
      781 aatccgcatt ttcaagcagc ccggcgatca gcaacttgcc gtgctgaatg gccaggggtg
      841 gatctacggc ctggtgctcg atcctacggg aaagtatttg gcagccgtgg acgatcgtgg
      901 actgctcacc gtatggcaat gggaaacagc ccgccagctt tatcgcctcc cgctgtcaac
      961 cgccgcactt cgtacggttg cgtttcatcc agggggaaat tggctggcag tgggtggttt
     1021 tgaaccgcgg gtcttcatcg tggaaaccct cacgggacgg cgagtggcgg accttaacgc
     1081 cgatcgggat gtccgccagg tggtattctc cagcgatggg gcttctgttg tggcagcagg
     1141 aggctcgggt aagctacggc tttgggatct cccaagctta cggcagcgga tggaaattac
     1201 cgtatcctct cgacgtttgc acgcggtagc attttctccc gacggaagaa tcctcgccgc
     1261 tggaggtgag gagcccgtcg tcagtctgtg gcaggtcgat tgtgatcccc cttcgttgat
     1321 cgggcgactc ccgctttcgg acggcatcgt caccagcttg accttctgtg aaaacggtca
     1381 acagcttgca gtcgggacga cacgaaacat catccacgtc tttgaccttg tgacgtccaa
     1441 ggaactggcc caattggagg gtcatacggg cacaattagc tgcctgacat ggacgccgga
     1501 acgctcctta cttagttccg gaagttttga tacgaccgtc cgcttttggc ggctgctacc
     1561 cgggacaact gtttccgagc gtcttcccac tgcccaggat atcctgcggc aatgaggcgc
     1621 ggctgcatta caaaacaaaa accgaaagga catcataaaa accaccttga caaggaggtg
     1681 atacgtgaaa ggttggaagg gacgccctca acaagcgccg tcgcaaaagc gccgggatcg
     1741 ccatccctcg cgttaccgaa agtgccggct ggaacagatg gaaccaagga tgctcctctc
     1801 ggtatcaccc atccagttgg ggatggtcta ttacgaggat gccaccggca ccgatgaaag
     1861 cggcgatacg tttgagctga cgttccaagg gggcgcgccg ggcacgcagt tgacacggtt
     1921 ggtgatcaac accgataagc tgggagatgg cttgtccatc ggggactgct tttttgacac
     1981 cgaacccggc gggctggggg cctttggtta cggtcccttt gcggtgatct cggccagcgg
     2041 cattgacaat tttcgggtaa gcgtatcaga cggcggtacc gaactcgtcc tggaattcac
     2101 aggctttgag gcgggcgacc gcttcatttt caccatcgac gtggacgaaa tggggttcct
     2161 cggtcctaac gcggttgccg aaggcaatga gtttgaaggc tcacgtttaa cggcctattt
     2221 cgtggctccg cactattatg cggcccaggg tgaagacatc ttcattgacg cttacgacca
     2281 caagttatcc gcttcggggc tggtccttcc ccacgattcc tatagcccgc cgttgccgga
     2341 acctgaaccc gtccgcacgg ccggggcctt tctggaagtg actcagacgc ctttgccggt
     2401 tgccatctcc ggccgggtgt tcgaagattt taacctcgat ctcaccctca acggagagga
     2461 caggcctttg gccggtgtta ccctcgaatt gtggcagtgg caaggcgaga cctatatctt
     2521 cacgggacga acgaccgtca cggatgccga cggcagctac cgctttgacg atctcctccc
     2581 cggcacatac cgcatcgtgg agcgtcagcc cgaaggtttt ctcagcgtcg gtgccaaagc
     2641 agggactgtg ggttcagagg tccgcggctc ggtgctgacc tctgatatca tcgtgggaat
     2701 ttcgctctta ggtggtgagg agagcatcga gaataatttt gccgaggttc ggcctgcgga
     2761 gctttccggc cacgtttatc acgacgttaa caacaacggg ctactggagt ccggcgaaac
     2821 gggaatcggt ggcgtcaccg tgattgtcat ccgcgaaggt gatcactttc tgaccgagca
     2881 agagttcacc gtcatcaccc agaccgacgg aagctggtcc gttgtggatc tctatccggg
     2941 gaaataccgc gtgcaagaaa tccagcccgc cggttggctt gacggtctcg atcgcgccgg
     3001 ctcgctcggt ggctcggccg tcaatcccgg tgatcagatt gtcaacatca aagtcccggc
     3061 gggaagccaa ggggttaact atgacttcgg cgagcttcta ccagcctcgc ttgccggcct
     3121 ggtgtatctg gacctaaata cgaactgcca atatgaccca agcgagccgg tcctccctgg
     3181 ggtgaccatt aagctgctgt cgcccgacgg tactgtcctc cggacaaccg tgacagatgc
     3241 ctctggccgg tatgtctttg agaacctcac gccgggcact tacgccctcc atgaaatcca
     3301 acctgagggc tactttgacg gctgtgatcg accaggcacg gccggcggcg tcaagtcccc
     3361 gccggatttg atcacggata ttcacctcgc gtcgggcacg gccgccactg attacttatt
     3421 tggagaaatc ccccccgcat cggtcgccgg tttggtgttt tccgacctta acgccaacga
     3481 tcaacttgat gccgaggaca ccccaatcgc cgcggtgacc atccaattgt tcgccgccaa
     3541 cggtgaactg gttgccacca cagtgacgga cgccgaggga cgctaccggt ttgagaacct
     3601 gcgccccggt tattacacca tcgtcgaggt tcaaccggta ggctacttag atggtgccga
     3661 tttcgtaggg agcgccggtg gtcaacgcga cggcaatgac cgcatcacgg acatcaagct
     3721 tcctcccggt gtccacgcaa cggattacaa ctttgcggag attctgcccg ccacgattag
     3781 cggttacgtc tttcaggatg gtcccaccat tacctacgtg tggggtcagg agcgtccgga
     3841 tccatacctc gttcgtgatg gccaattcac cccagatgat attcccatta gcggagtcga
     3901 aatccagttg cgggatgccg ttgggcaacc aatcgtgggt gaagatggtc ggccacttgt
     3961 gacctggacg gacgaacgag ggtattacga gtttcgcggg ttgaggcccg gcgtttacac
     4021 cctcttggaa atccatccgg caggttacga ggacggcttg gacacaccgg gcacgctggg
     4081 agggattgcg gtcaacccca atgcctcgtt ggatcctgcc atccttgccg ccctgaccat
     4141 cgacccgcgg aacgatgcga ttctgcaggt aactgtgaca ccgggtgccg ttggaacaaa
     4201 ttacaatttt gccgaggtcg ttgtgacctc cgttccgccc tatgtgccgc cgactccgcc
     4261 cccgccgtgg tcgccaccgg aacggccggc ctcgcctgtg gttcctcctg ggacaccacc
     4321 gattcacctg caaatttccc ccgtgatgcc cgaatcggtg gccctgccgc gattcggagg
     4381 aagtgccgga acggtagact acacctggca tttgagcgtg atcaatggtg gtgttcctcg
     4441 gcacaatctc ggcggatccc aacgggtctt gatccgagag gtcagcctat tcaatccggc
     4501 gcggtgggag gggattccgc tcgatcgcgg ccaatggaca atcgtcgata tcgtcactgg
     4561 ggaaactgcg gtccttggca atctaggtac cgaggccgct ttgcccgtgg tgggagattt
     4621 cagcggcatc ggtcgcgtac agctcggcat cttctacggg gggatgttct tcatcgacct
     4681 caatggtaac ggcatttggg acgaccaaga cctctggatt cagcttggca aggaccggga
     4741 tcagccggta gccggggact gggacggcga cggaaaatgg gacgtcggca tctttgggcc
     4801 cgcttggccc ggtgatgacc gggccctgcg ggcggaaccg ggcttgcccg acgcccaaaa
     4861 ccgcctgacg gcctttcgtc cacgaaacct gccacccact ccagaccagg caccggacga
     4921 tcaacgcctc ctccagcaag ggcgaaacgg acccacccgg gctgacctca ttgaccacgt
     4981 gttcatgtac ggtcaaccgg gcgaccacgc cgtggtgggc gactggaacg gcgatggtat
     5041 cgccaccgtt ggcgtcttcc gcggcgggac gtggtatctc gatgaagatg gcgatggtcg
     5101 ctggggaagc cgcgatcggg tagccttctt cggccaggcc ggcgatctcc ctgtcgtcgg
     5161 agattggaat ggcgacggaa tcaccgacct cggggtgtat cgtgatggcc gattctatct
     5221 cgatatcgac ggcaatggcc aactgacagc cgccgacaaa gtcatcacct taggacagcc
     5281 tggtgatcgc cccttcgccg gtgatttcaa cggggaccgt cgcgctgaag ttggcgtcta
     5341 tcgcagccag aaccctacta gtggcgggag tcaaaccctt cgggaggcag ccgctccccg
     5401 cggtgttgcc aaatgacgcg tgacgagaat accccattaa tgggggacat cctttccaat
     5461 tttacccaaa acggcgtctt ccccgtgaag acgccctatt tagaacgtat cccaaaatct
     5521 cccctctccc gttcgggggg agaggggtcg ggggtgaggg tggctcgaat cgctgccggt
     5581 tttctcgatc gactccgacg gtcccttgcc ctcaccccaa ccctctcccg gagggagagg
     5641 tggctctttg ggatggactc ttatttccta ggctaatggg caaaatcgtt gcggcagtag
     5701 cccccaggct gccatggaat ccactccaaa aaattgatct tggccggttt tgctggattt
     5761 ggtaaaacat tccacgatcg ccagattatg cgttcttctc tagacaagga tgtcttgcgt
     5821 gcgggcgaac caaggacggg aacgcccggc ggtccggcca gatgattcga cggcaacggc
     5881 tgaatccaaa gcgttcgcca ttcgcgagga tcaactgagg agtctcgcag taagcctgaa
     5941 ccgtgccgtt ctgcggggga tatgtgcatc tgcaagatcg tgcctcgaac gtctgtgaag
     6001 gatttggcaa gcgggatcca agtgtagaac ccggcccaca caggccacgt ggacaagtag
     6061 caagggaaaa acggaccgcc aatcaaggct tgcgcataga ggatgagaac cggagccgcg
     6121 cagccgctcg cactgcctgc cgcatttgtg agcgcgatcc gggttgcgga aggtggagac
     6181 tggattcgtg gtgcaacccc aagatgcgta gagggaacga ccatgttggt gctaactcgc
     6241 cgagaaaacg aacggattgt cctgcctact ctcaaaactt ttatccaggt ggtgcggatt
     6301 gccggctcaa cagtccggtt agggatcgag gcgccggcca acgtacctgt tcttcgggag
     6361 gaacttctca aagaaaacag agcacccgag gatactgcca ggaaacccca ggtgccgtgt
     6421 ttggattcct tccctgagac gctcgagcat tcgcatgacg ccgccagtgc cccagcgacc
     6481 cgtctgtcgc agcaggaaaa actcttggat tgccttgcct tggcctgcga tcttgcggac
     6541 acccatttgg caaatggcaa ggtggctgag gctcgcgaaa caatccaaaa ggccctggag
     6601 cttcttcgtc gcctgggggc gagatcggtg gtggctgctt cctccccggt tcgggtctcc
     6661 ggtcagcgcg ttgcccttgt cattgagccg acgaaaagcg agggctcttc cgtgtcgcaa
     6721 gtgttgcgaa aggcgggtta ttcggtaatt tccttcgcca gtggctgccg tgcgattgat
     6781 tatcttgcct cgcataaccg gcccgatcta attgtgatca acctacagga aaaccgacct
     6841 tgcgcggccg ataccatccg catgattcgc caaaacccgg cgctccgcgg cgtcaaggtc
     6901 gtggccctga gcgattctcg tccggccgag gccgaaataa gcatcggtcc ccaaggcgtc
     6961 gattactggt tcgccaccca aacctgcccc gaacgaatcc tccgacaaat tgaggcataa
     7021 gcgatttttc aactgttttc gtcgtgtctc cgcgcggcgt cagaccgctt aacgagtcac
     7081 caacgaattg gcaacgaatg gacgtgcaac gtgattgagc gggatacact gtttcggcta
     7141 tctgacggtt cttcgttgcg tgggcgatct tgggaaccgg agggaaaccc tcgtgcccga
     7201 atcttcggcc tccacggtgt cctggagcat agtggccgat acgggcgatt ggccacggat
     7261 ttgacccaag ccggttacgc attggagatg gtggaccttc gcggccacgg tcggtccgat
     7321 ggcccacgtg ccttcattcc aagctgggag caattactgg ttgatctaga cgaagtccat
     7381 gcccaattgc gggcggaagt gcgagtgccg gactttcttt ttggacacag tatgggggcg
     7441 gccctcacaa tcttgtgggt gacgtctcgc cgaccgaaca taaaagggat gattttgagt
     7501 gccccaccgg tcatgacccc agttcgggtg cccatgctcg tgcgtatcgt tggccgttgg
     7561 ctggtccatc gctggccaga gttacgcatc gtccggctga aaacggcgga gcgatatggg
     7621 cgacacgccg tgtcccgcga tccccaggtc ctcgaagact tgaaaaacga ccccttggtg
     7681 tgtcgtggtc ctttcccgct gaagaccggg ctattgctgc tggagttgcc ccaacggttg
     7741 caggaagccg cccgggcgtg cgacgccccg tttcttcttc tccagggtac gggggataag
     7801 ttgtcgtcct atcgaggggc agaattattc tttcaacttg ctccggcaag ggataaagtc
     7861 ctcaggttat acgagggcct gtatcacgaa gtcctcagcg aaccggaacg tgcccaggtg
     7921 ttggcggacg tcttgtcttg gctttccgcg cattagtcgc ccgaaaaaga tcttcctgcg
     7981 gaaaataggt agccaacgcg cgggcggata tctgaatcat tttgatatca cccacaatgg
     8041 gcgggggaat aattggcttt gaagcgtgat ctatattggt gtagggtcca ggttcccact
     8101 ttgcaggcgc agaacagcca ttgatggcga tctcctaatg gacacggcct catggaatgg
     8161 gtaccttaaa aagggcgagt cggacaaacc cccgctcata gaaatacacg aaccggcggg
     8221 attgttttca actgaccgag ttggtcggtg gccgccggtt tgaggagggt taactcaatc
     8281 tgttgtggag gtctaactca tgaagcggtt acttgcatta ctgttaggga tcggcttgtt
     8341 ggtttacgtg acaggctgtc cgaaaccggc aacgaagcca gaaggcggca cgggccccgc
     8401 gccttcgacg actgccccgg caggctctac ggaagagcaa ccggctgaac agccggctga
     8461 aaaacccgca gagcaaccgg ccgagcagcc agcggaacaa ccggctgccg gtcctgagtt
     8521 gccggctccc gcagcgccag ccgaggtgac catgccagct cccccggcag aacaaccagc
     8581 ccaacaaccg gcagaggaac cagccgctca accggctcag cccgctgaac agccggcccc
     8641 tgctgccgag caaccggcac ctcagcctga gcaaaagccg gaagagaagc ctgccgagca
     8701 acctgctgag caacccgccc agcagtgatc gcaaatcggt cagactagtg atctgactct
     8761 accgactgag gtctgccctc gttcgcaggc cgggtaaatg ttgcaaattt accgggagta
     8821 ttggtttagc gagcctctca ctaaggctcg cttttttgtt gagaaccgcc aaccgggaat
     8881 aaagcccctg atcggaggcc actccaatat cctgtccgcg gccggagaac atccgccaac
     8941 cccaccccct gatgaccggg cacggccaca gctcttctcc tgtttgatac aataaacttt
     9001 gcgattgaaa cccctccctt ttgagactga attcttccac gcttatgccg acgctccgga
     9061 cggtcacgct gggttgcaag gtcaatcagt acgagaccga ataccttcgc caaggctttt
     9121 tgcgactcgg ctatcaggat gctgagaacg gggagcctgc ggacctctgt attttcaaca
     9181 cctgtaccgt cacggcccag gcagacagcg aatgtcggaa aatcctccgc cgccttgccc
     9241 gcgagaatcc ccacgccgag atcatcgtga tgggctgctt tgccacgaag gcggccgagg
     9301 agatccggtc cctgcccggt gtgtcggagg tccttactga taaacgccag atgggcgatc
     9361 tcctggcccg ccggggcctt gtcgatattc ccacgggaat ctcgggtttc gccaagcggc
     9421 atcgggcata cgtcaagatt caggacggtt gtgtctttcc ctgcagttac tgcattattc
     9481 cacgggtacg gggacggctg tggagccgcc cgctgcggga gatcctcaac gaagttgccc
     9541 aattggtgga taacggctat cgggaaatcg tcctcacggg aatccatttg ggactgtacg
     9601 gccacggtcc ggacgacacc gacttacccc atttggcggg gcttgtggaa gccattatcg
     9661 acagccgcag cgggttccgc gtccggcttt ccagtctgga ggttaacgaa gtcacggata
     9721 gtcttttgga cctcatggcg gcgtcgcccg aacggatttg cccccatttg cacattccct
     9781 tacaaagtgg ttccaatcgc gtccttaaaa ggatgcgacg gcgtgccgat gtgggttact
     9841 ttatggagcg ctgcgccctg gtccgcgaga aacttcctgc tccggccttg acaacagatg
     9901 tcatggtcgg ctttcctggc gaaacagatg aagatttcga ggcaacatgt cgggtcgtgg
     9961 aaacggtagg cttcgccaag gttcacatct tccgtttcag ccctcgcgag ggaacaccag
    10021 ccgccgagtt tcccgatcaa atccccgaaa agacgaaaaa agaccgggca cgcacactcg
    10081 acgcggttgc tgagaagaca agaaggcagt acatcaactc cctttttgga cgtcaacttc
    10141 aagtactctg ggaagacctg gctagcgctc ccggtgacaa ttctcctcag tggcttttgg
    10201 gaatgagcga ccgctacatc cctgtaagag ccttggccag ccgcgggaag caaggtgaat
    10261 tggacggggt aaccgtggag ggatttgacg gtacgttcct gctcgatcgg gacttgttcg
    10321 ctgtcacatc acctcccgaa atgtgtcaaa ataacgccca agtgggatgc gtcggttgca
    10381 attttgacga ttttccgaca tcataagtag caggcaatca gaagaaagag ttccgccttc
    10441 gcgggatgtc tcgcggacgc tgtcgggggc aggtaactga agaaaggtgt ttcggcacag
    10501 tcagagcaat tagggggagg ttcccgccgg gtggtgagta accctgaggt gccgaaaata
    10561 ggccttagcc cacccaaggt gagggtcaaa cgtatggcca caaaaaagaa ttcgtcaaca
    10621 tcgttggctt ccgaggagaa gcaggcattg gattcgccaa acgcacaggc cgtcgaaacc
    10681 cgccgtaagc ggatcctcat cgtcgatgac gatgtggaga tcgtcgagtc gattcgcgtc
    10741 gcgctggagt cgctcggcta tcaaatctta attgcgcgcg acggtaatca aggcctggcc
    10801 ctagctgatg ccgaagaccc agacctcgtg atcctcgaca tgatgatgcc caaacgcagt
    10861 ggtttccttg tgctggaaaa gctgcggcaa acccgcaaag tccctgtgcg ggtcatcatg
    10921 atcacggcca acgaaggaag tcgtcacaaa gcctatgcgg aaatgctcgg cgtggatgat
    10981 tacatccgca agccgtttgc catggatcgt ttgatcgaaa gcgtgcagcg gctcctcgaa
    11041 taaccctgcg gatggataac ccaccgcggt cccggcacac cccttgggag gattcaggcc
    11101 atcgagggga tcatcaaatc cggattccct tttgcgcagc caagtagata atcgctcgtt
    11161 tgaaatactg gaacttgtac tttggcctct tttgcgccca aagataggcg gactggagaa
    11221 tttgggcggg cagttgtcct tgttgcacca atccaaccac gtactccaca aagccttctt
    11281 cctctggtgt ggccgtatga agggccgctt tgataagttc ggcattgagc cactcggcgg
    11341 cttcaaccgc ttcggagagt tgcgcgactc ccactagccc gctgccaccc agtatcatca
    11401 acctcataaa ctctcgccgg cgcataatgg gggaacctct ttcttcttga tctcgcgttt
    11461 caactggggc gctaacccgg agacattctc tcccgaacgc taacgggggg catcctaccg
    11521 atttttgaat cctacggcaa gggaaatcgt tagtaagacg ggcaacactg gcccaacgta
    11581 tcgcaagatt tgggctagcg ggatttgcaa aaaaagttat actccgaccg tcgctgggca
    11641 cgttcggtta tccccagagt cctcgaatca ttttcaggtg gtcatgtgca ttgtttttca
    11701 caaaaagcga gccaaggtaa cctatcggaa cacggataac tcacctcagg gtgaaagacg
    11761 cggcagtggg tggctgctcg cgattttggc cctctctctt gtcctttccg ccgggtgtgt
    11821 gcaacggcga ctcattatcc gcagtcaacc gccgggggcc atggtctatg tcgatgatta
    11881 ccagatcggt ccaacgccga ttgccacatc attcacgtac tacgggacgc gaaaaatccg
    11941 gcttgtgaaa gatggttatc aaaccttgac cgtctatgag cggatcgacc cgccctggta
    12001 tcagattccc ccgattgact tttttgccga aaatgtcctg ggacgagagg tccgcgacac
    12061 acgggtcctg gaataccagt tacaacccca accgatctcg cccacgcagc aactcctcga
    12121 tcgggcggag acattgcggc gacagacagg cggttctccg cccttacccg cggcagcacc
    12181 gatccaacag acacagcaac cggggatgcc atccggtgcc ccaccgaccg agggtagcac
    12241 cgcgccggcc tttatcattg aaccgcctcg gacttcctat tagtcatgag tgaagtcgaa
    12301 gagcacgctg tgcagaatca gacctcaacg agcatattcg aacgatcctc gcgggcaccc
    12361 ttacttcatt tcaaaaa
//
LOCUS       DAOSUT010000126        12154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3029_length_12154_cov_8.931234, whole genome shotgun sequence.
ACCESSION   DAOSUT010000126 DAOSUT010000000
VERSION     DAOSUT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_12154_cov_8.931234"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..525
                     /locus_tag="PLD05_11475"
     CDS             307..525
                     /locus_tag="PLD05_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOP78103.1"
                     /translation="MLRLLLNDRTKRLFSTFKRLFRDETAATAVEYAVMLALIIAVMM
                     GTISQFGSETHGLWSGSIDRLTAAGFMK"
     gene            complement(652..1158)
                     /locus_tag="PLD05_11480"
     CDS             complement(652..1158)
                     /locus_tag="PLD05_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78104.1"
                     /translation="MRPINDGARRNMLCHNTRDELFRLLDALLNETISEEQFRRLEAI
                     LLAEAEARQIFRRYLTLHHQLRTLAKSWAEEKLVKLAEGPNPEVPPVSPSGQMGQGIL
                     YGFLIAIGLALLAGALWFRHIRAETPCPPTTFSESISHAPLVCDSPGSCRLSDKMASR
                     PSEAQPTK"
     gene            complement(1244..1816)
                     /locus_tag="PLD05_11485"
     CDS             complement(1244..1816)
                     /locus_tag="PLD05_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP78105.1"
                     /translation="MGYDSYALETRDWVELAAAGTDELTKDFLEQFTWHHERIFAYIF
                     GLVHNEQDARDIFQKVSLLLWQKFETFDQEADFFAWACGVAYNEVRNYFRTSSRNRLC
                     FNQELLDTLATERDDEDDESDARLEVLRDCLAQLPQHERELLMEVYAEGRSIREIAER
                     RGKAAQTLYNRLNLIRRKLFIEVQQALAQN"
     gene            3689..4450
                     /locus_tag="PLD05_11490"
     CDS             3689..4450
                     /locus_tag="PLD05_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78106.1"
                     /translation="MTRRIVICAVLVGIALFATRRADAQANAPEAWQNETYVAAASQT
                     EIARGQAEMIKAYAAILQAQAAMVKAQADAAVAIANARKVSMEAESARLDNTLKYAKV
                     YWDKKQARDTYLALRNTPTASPGESKHAANNNKPAGQLASAQVARVQRLTPAELDPVT
                     RMIHWPAALQGDRYAKQREALEELFTNRQPNEHGAASPFFAKVRENVDVMKNLLREEV
                     TKLSPAEYIAASKFLDGLRNEALYPAQINGVAMNQ"
     gene            complement(4656..4868)
                     /locus_tag="PLD05_11495"
     CDS             complement(4656..4868)
                     /locus_tag="PLD05_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78107.1"
                     /translation="MRKNDGQENDSPRFSWLHNVDVPFDWLLGTVTGEVGLADSVDML
                     QFDQLFPRLLAHSLSVTGTGVIAFGI"
     gene            complement(4899..>5690)
                     /gene="hemG"
                     /locus_tag="PLD05_11500"
     CDS             complement(4899..>5690)
                     /gene="hemG"
                     /locus_tag="PLD05_11500"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOP78108.1"
                     /translation="RESGARYSMFVTLQQGLQELVDRLAQHLPPGALRLGVGVTTIRR
                     ENDHWVLTLTDGHQFVGDAVVVATPAYAAAPLLCELDGRLAEELAAITYTGSVILAVA
                     YRREAVGHPLHGSGCVIPRIEGSPLLAVSFSSQKYPHLAPKGYVLLRVFAGGARHPEL
                     VSLEDTRLVPLLRKEVERLLRIEGQPVQHWLFRWSQAMPQYEVNHGDRLRRIAERLAA
                     WPGLALAGNAYRGVGIPHCIQSGRQAAVRALGLPVEDQQTSSSAF"
     assembly_gap    5691..5790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5791..6239)
                     /locus_tag="PLD05_11505"
     CDS             complement(<5791..6239)
                     /locus_tag="PLD05_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP78109.1"
                     /translation="MENTLPSPDAPARPFRVAVIGAGISGLAAAWQIRRLRPEAELAV
                     FEQQHSVGGVLGTVRENGFQVERSADNFITTLPWGVQLCRELALEDQLVTTHPDHRRA
                     FVVWRGRLHPLPDGFLMMAPTRLWPLATTPLLSPLGKVRAAMEFFIPP"
     gene            6426..7745
                     /locus_tag="PLD05_11510"
     CDS             6426..7745
                     /locus_tag="PLD05_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOP78110.1"
                     /translation="MNTPGSLRWHFLYSTMAAVIGLAVALSWSTSSWAMHREQHIFSI
                     VDFGAVPNDEQLDTESVQKAIDKAAEVQGTVRIPAGRFILGNVQLRSGIRLVFEEGAV
                     IQGSSDWRHYGQGRWTDSLFLGEDITNLRIEGPGTIDGADCERPGGEEGFRGPHGIRL
                     VRSSGVVINDVTIKRAGNYAIICWECRDVQVKGVTFRGGHDGVHAQACQNFVIEACDF
                     RTGDDCIAGCDNEHFVIRNCQINSSCNGFRLGCVHLLVENCRFWGPGEYPHRITVRQG
                     KPRTNMLSAFVHFAPTDRNPKLPSDDWLVRNCTMTNVDRVYSYDFEKGVWQTGQPAKR
                     LVFENIQAEGIAFPLNILGDKDRQFDLTLRNVAISLRPASAQQPVIQARQFGKITLER
                     VVLKNDGSQPVLRAREGNELILQNVAYPPECSQPFLLEDIVRIDRRP"
     gene            complement(7895..9844)
                     /locus_tag="PLD05_11515"
     CDS             complement(7895..9844)
                     /locus_tag="PLD05_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HOP78111.1"
                     /translation="MSESREIVLINMPFAHLRWPNLGLGLLAGALKRRHLPCRTLYLN
                     FALAEEIGYDLYEWIADHYAFVLGGERLFSQHYFGQLPTDHQAYYEEVLHRADRDLTR
                     EEYAEYLAIFPKIERFIDAAFDAYPWQDAALVGFATTFQQTLASLALAQRIKRASPET
                     VIVFGGAACEDEMGVELLRQFPEIDYVFLGEADETFPELAEAVLGGRRPPILPGVVAR
                     ANMFGPSLQGTSESESVTGQPASPILPQLHILRGPRSVLVENLDALPYPDFDDYFATL
                     QRSPLRHELTPMLFFEMSRGCWWGEKHHCAFCGLNGQSLRFRSKSPQRVIDELRYLVT
                     RYDVHQGCAADNILDFRYFASLLPELEKAQLAFSFACELKTNLKRSQIEQLLRAGLRA
                     AQLGIETFDSEILKNLNKGVRGYQNLQTLKWFAEARIAAEWNILYGFPWETADTYRQL
                     ASLVTKIPHFPSPLGVGRARMDRFSPFFESPGRYGMKNPRASRAFAHVYPFPQDVLNR
                     LAYYYEFDADWEPQLEFAEPFLEAIRHWQNDSGSGDLTMHWKTRDELVIFDTRGGRMR
                     QHRLRGLEAELYVTCDTAQTLQTLTQVVATRSITPENLKALLSQWLEDDLMIYWDGRF
                     LSLALWVPEQSPLYQAAQANLEPIP"
     gene            complement(10188..10688)
                     /locus_tag="PLD05_11520"
     CDS             complement(10188..10688)
                     /locus_tag="PLD05_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOP78112.1"
                     /translation="MSRFIQQRHRQPITLERPRELIVACPPMKSNVNLSRIVRAAGCC
                     GIRRLICCGNARILPEIARDTGPDLQIEFHRSLLPVLKKLKAEGFPLIGLEQTTGSIW
                     IYEFAFPRRCVLVVGHERLGIEDDILAILDHVVEIPVYGLPFAHNVATATAIALYEYC
                     RQYPKG"
     gene            complement(10685..11161)
                     /locus_tag="PLD05_11525"
     CDS             complement(10685..11161)
                     /locus_tag="PLD05_11525"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOP78113.1"
                     /translation="MWTPLHAGLGGTDTPQINAAPENGAAAQLEELLASLTWFDRILL
                     RVGIRWLRRIAITIIGLTVVLVGIIMIVAPGPATLVIPIGLAILGLEYAWARRLLRRL
                     RGYVDAGITHGSALLGWKTPLVPSPAGTTPVQPPNESANTSQTPNTSTSPATTSSP"
     gene            11592..11954
                     /locus_tag="PLD05_11530"
     CDS             11592..11954
                     /locus_tag="PLD05_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOP78114.1"
                     /translation="MDVQNLAHVPAFITKDGSEIRELLSYRNSCIRNQSLAEARLPPG
                     RSTQGHHHVRSEEIYYILEGEAIVQVGEEKRQVGPGDAVAIPPGAYHQITNTGSTVLR
                     FLCCCAPCYEHDDTVLRD"
BASE COUNT         2814 a         3325 c         3397 g         2518 t
ORIGIN      
        1 tttccaccta aagattgtac cgatttgaac cttttcgcgc aggccggaag tcgcggggca
       61 aacataggaa gcgatgacga gcacgggagc gatgggcacg aagcccaccc tccaccggtg
      121 cagatcggtt aacgccagcg tctcccaggg caagcttagt ggtcgcccaa atgttgaagt
      181 tgatgggtga tctacacatt cccggtccgt ggggtatttt ttgaccccca cccggtcgcg
      241 agctatgttt ttaataagga cttggggacg aagataaacg attcttttcc gataaggcca
      301 ttgaccatgc tccggctgct cttaaacgat cggacaaaac gtctcttctc gacgttcaag
      361 cgtctgtttc gtgacgaaac ggccgccact gccgtggaat atgccgtgat gttagccctt
      421 atcatcgctg tgatgatggg caccatcagc cagttcggca gtgaaaccca cggcctatgg
      481 tctggttcca ttgaccgttt gacggcggcg ggcttcatga aatagacgtc gttgaggcga
      541 gaatccgcca gttgaagcgg cttcgctcaa gcgaagctga tgtgcgctac gttacttgac
      601 aagcgtgtcc cagcaattgg ccggagaagg gattcctctg tatgggaaca cttatttggt
      661 aggctgtgcc tcggaaggtc ttgacgccat cttgtctgag agccggcacg agcctggcga
      721 gtcgcaaacc agtggggcat gcgaaatgga ttcggagaag gttgtagggg ggcacggcgt
      781 ttcggcacga atgtgtcgaa accatagggc tccggccagt agggccagac caatggctat
      841 gagaaagccg taaagaatcc cctggcccat ctgaccagag ggtgagaccg gtgggacttc
      901 ggggttgggg ccttcggcca acttgacgag tttttcctcg gcccagcttt ttgccaatgt
      961 gcggagctga tgatggaggg tcaagtatcg gcggaagatt tggcgggctt ccgcttcggc
     1021 taagagaatg gcttcgagac gccgaaactg ctcttcggag atcgtttcgt tgagcagcgc
     1081 gtcaagcaac cggaagagtt catcgcgcgt attgtggcac aacatgttcc gtctcgcacc
     1141 atcattaatc ggccgcatga cgcccgctcc ttgcagcgcg ctacgacatc gccgggatgc
     1201 cgtagcgagg gctggactgc atcactgcat gggagtttgg ccgtcagttc tgggccaggg
     1261 cctgttggac ttcaatgaac agttttcggc ggatgaggtt gaggcgatta tacagcgtct
     1321 gggctgcctt tccgcgtcgt tccgcgattt cgcggatgga gcgaccttcc gcatagactt
     1381 ccatcagcaa ctcccgctcg tgctgaggaa gctgtgccag gcaatcccga aggacctcca
     1441 ggcgagcgtc gctctcatcg tcctcgtcat cccgttctgt tgcgagcgta tccaaaagct
     1501 cttgattaaa acagagacgg ttgcgtgagg acgtgcggaa ataattcctc acctcattgt
     1561 aggccacacc acaggcccat gcgaagaaat ccgcttcctg atcgaacgtc tcgaactttt
     1621 gccacaggag caggctgacc ttttgaaaaa tgtcccgcgc gtcctgctcg ttgtggacaa
     1681 gaccgaagat atatgcaaaa atccgctcgt gatgccaggt gaattgctct agaaagtcct
     1741 tcgtaagttc atcggtccct gccgcagcaa gttccaccca atcccttgtc tccagggcat
     1801 agctgtcgta tcccatcgca cggctcctcg ttcattgttc caacgctggc ggctcgcaca
     1861 gcgattgccg gcttccacgc gacaaatgcc gtccctggct gagaaatcag gggattgttt
     1921 tcacccgagt tggtcttgtg tttcgcctga tttggggggt gaaggatctt ctatgaagct
     1981 caatcaggac tgacaaatat cgttatgacg ttccgtcctt tgatagcact ggttcagtct
     2041 cctaccccag gtgcacgtac aggtgggatt tgatttcgag gcaggcaacc tttcttggtc
     2101 caaagattac tcgtacatcc taggcttatt ttaccgacta aaacggaaaa gacagtgccc
     2161 tttgcgagaa aatagcggaa aaacaccgct gcagtgggga ttagattact aattggcatg
     2221 ggatttgagc gcagggcgta gcggggatat cccccaagtg atggggtctt ttggcctact
     2281 gctgctcgta tttgtcgaag tgtactcatg atcactacac gtccggtgtg acgaatttgc
     2341 tttgttgtaa cacgctagaa gctggccgtg tctcgtggaa ggctcagata agtttacacg
     2401 gaagcgacta tcgccatcgg acgttttgtt gggcgtgtcg caacggccag catgcgatcg
     2461 cttcggcgag agattcccgc aaatcatctg gtaaagcgga aatcaatcac ggtatctata
     2521 gcgcgacgtc tcctcgctgc tcgacaatgc caaagaactg gaggctccgt cccgtgcatc
     2581 aatttggggt tgcgttcggg aaaaactctc ggtaaaagtt tcccccgttg tacgactaaa
     2641 agacggcggg cgagaaaaag cccgaaggaa caaaggaaaa acgttaaccg cttgtgccga
     2701 gatttccgaa gatgtcaaag gggacatgca aatcggctgt cccgaataca acaaagaatc
     2761 tcgaaaaatg gccttgcggt gagtcgtagg aaaggctaaa acccaacccc aacaacgcgg
     2821 ctaacaataa caaccgttcc aggcgaaagc cggccgcgtg atgcacacca agaggaaatc
     2881 agttctgaga attatcacct ccctgaaaat acccacctaa acattccccc ctgtggacag
     2941 cggcagacct atcttgggaa gagagtcgtg aacagcaggt tgccgcagcg gaaacacaat
     3001 aagtgtcaca ccataggatg tgaagaacat ccgccttcct gccggtcggt gaaatctccc
     3061 gcctaaccga ccccctgagg catcgcctcc gccctgggag gccgtcgtgg gtccggcggt
     3121 ctcccaggcc gacgatcgaa gtgaaatcga ccgacacaac acgaggacga tacccagtta
     3181 cggacaagca gcgaacaata aaccgaagca gtgagaatta gggttaacac actactacac
     3241 aagagaacat acacaaggtg aggcggtcaa acgacctcgc caccagcaaa agagaaaaca
     3301 acactcgggt ctgaaaagcg gaatcgtcat ccgagccaag aaaggaggtg ctacgacaag
     3361 gacagagagt gactgtggca tgtgacgaga agttcacccc gaacgactag ggtaaaggtc
     3421 ggctgcgtgc tccaaagaaa agatccatgt tcctccccta agtaaagttc ccaccgacaa
     3481 accttccaag aagtcccacc tccaaatctt tcatcaagtg cgacagcatc accgtagaga
     3541 aagttcggaa ttgaactagg gtattcacac tatgtgtcaa gtatgtttcc gactttcctc
     3601 ggtggtccca cggtgacaaa aaagcgtccc taagttttgg taagtaagac gtttctgttg
     3661 gtccttccca gccagaggag atgtagccat gacacgccgt atcgtgatct gcgccgtttt
     3721 ggttggtatc gcgttgttcg ccacccgccg tgctgatgcc caggccaacg ctcccgaagc
     3781 gtggcaaaat gagacctatg ttgcggcggc gtcacaaacg gaaatcgctc gaggacaggc
     3841 cgaaatgatc aaggcctacg ctgccatcct gcaggctcag gccgccatgg tcaaagcaca
     3901 agccgacgcc gcagtggcaa ttgccaatgc ccgaaaagtc agcatggaag ccgagtccgc
     3961 acgcctggac aacaccctca aatatgccaa ggtctactgg gacaagaagc aggcacgaga
     4021 cacctatctg gccctgcgga acacccccac ggccagcccg ggagaaagca agcatgcggc
     4081 caataacaac aagccggctg gtcaactggc ctccgcccag gttgcccgag ttcaaaggct
     4141 cacgcctgcg gagttggatc ctgtcacccg gatgattcac tggccggcag ccctccaagg
     4201 cgatcgctat gccaagcagc gcgaggctct cgaggagcta ttcaccaatc gtcagccgaa
     4261 cgagcatggt gctgccagcc ccttcttcgc caaggttcgg gagaacgtgg atgtcatgaa
     4321 gaacctgctc cgcgaggaag ttaccaaact ctcccccgcc gaatacatcg cggcctcgaa
     4381 gttcctcgat ggtctccgaa acgaagccct ctaccccgca caaatcaatg gcgtggccat
     4441 gaatcagtaa ggctcaccaa tgtgtgacag tcagcctacc ctacagcaaa cctaaacccc
     4501 aacaaacctt cctcaacatc ctacctgaaa agtcccacct ctgagttaac caaccttgag
     4561 tgtcctacct gaaagtaact gaacgtgtga cgagccaaga gtcctagcaa agtgcagcat
     4621 ccttcctaag tgtggttcgt cagtcctgcc gaaaatcaaa tcccaaacgc gatcaccccg
     4681 gtccctgtaa ctgacaacga gtgtgccaac aatcgtggga acaactggtc gaactgaagc
     4741 atatccacgc tatcggccaa accaacctcg cctgtaactg ttcccaacaa ccaatcgaaa
     4801 ggcacgtcaa cgttgtgaag ccaggaaaac cggggtgagt cgttttcttg gccgtcgttt
     4861 tttcgcattc cacctggaca gccgctgggc aggacagatc aaaatgcgga agaacttgtt
     4921 tgctggtcct cgacaggaag tcccaaggcc ctcactgccg cttgccgacc gctttggatg
     4981 caatgaggaa tccccacgcc gcgataggca tttcccgcca aggccaggcc gggccaagca
     5041 gccagtcgct cggcaatccg acgcaaacga tccccgtgat taacctcgta ctgcggcatg
     5101 gcctgcgacc agcggaagag ccaatgttgc acgggctgcc cctcaattct cagcaggcgt
     5161 tcgacctcct ttctaaggag cggaacaagt cgggtgtctt ccagagacac gagttctgga
     5221 tgtcgggcac ctccagcgaa caccctaagt aggacgtaac ccttgggcgc gaggtggggg
     5281 tacttctgac tgctgaagct gacggccaga agggggctgc cttcaatccg cgggatgaca
     5341 catcctgagc catgcaaggg atgccctacc gcctcgcggc gatacgcgac agcgaggatc
     5401 acgctgccgg tgtaggtgat ggcggccaac tcctcggcga gacggccatc cagttcgcac
     5461 aaaaggggag cagcggcgta ggctggggtg gccaccacca ccgcgtcgcc gacgaattga
     5521 tgaccgtcgg tcagcgtcag gacccaatga tcgttctcgc ggcgaatcgt tgtgacaccc
     5581 acaccaagcc gaagggcacc tgggggcaaa tgctgggcaa gtcgatcgac aagctcttgt
     5641 aacccttgtt gcagtgtgac gaacatgctg tatcgggcac cgctttcccg nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggggaatga agaactccat cgctgcacgc
     5821 actttgccca agggcgacaa aagaggcgtc gtcgccagcg gccaaaggcg ggtcggcgcc
     5881 atcatgagaa aaccatcagg cagcgggtgg aggcgtcccc gccacactac gaatgcccgg
     5941 cgatgatcgg ggtgagtcgt caccagttgg tcttcgagag ccaattcacg gcataattgg
     6001 acaccccagg gcaacgtggt gatgaaatta tcggcgctgc gctcgacctg aaacccgttt
     6061 tcgcgaaccg ttcccagcac acctcccacc gaatgctgct gttcaaaaac cgcgagctca
     6121 gcctcgggac gaaggcggcg tatctgccat gccgccgcca ggccgctgat tccagcacca
     6181 atcacggcga cacgaaacgg cctggcgggg gcatccgggg aaggtagcgt attttccata
     6241 tcgggtgatc ttctcaatct gttactcttg gcagacaata accggggaca tggtgttgtg
     6301 acaattgtct ctcccactat cgatcgatgg aaaacttggc ttataaccga tggcggtccc
     6361 atgccacgag aggcatggtc atctcgggag aacaccgcaa acaaaagatt gtggggaaaa
     6421 tcattatgaa cactcccggt agtctgcgct ggcacttttt atattctacg atggccgccg
     6481 tcattgggtt ggcggtcgcc ctctcttgga gcacgtcgag ttgggccatg caccgagaac
     6541 aacacatttt ctccattgtc gattttgggg ccgttcccaa cgacgaacag cttgataccg
     6601 aatctgttca gaaggccatc gacaaggccg ccgaggtcca gggaacggtt cgcatcccgg
     6661 caggacggtt cattctgggc aacgttcagt tgcggagcgg tatccggttg gtgtttgagg
     6721 agggggcagt catccagggc agtagcgact ggcgacacta cggccaagga cgctggaccg
     6781 attcgctctt tctgggagag gatatcacga accttcggat cgaaggccca ggcacgattg
     6841 acggtgccga ttgtgagcga ccaggaggcg aggaaggctt tcgtgggcca cacggaatcc
     6901 gccttgtccg ctcgagtggg gttgttatca acgatgtgac gatcaaacgg gcaggaaatt
     6961 acgcgatcat ttgctgggaa tgccgcgatg tgcaggtcaa aggtgtgaca tttcgcggag
     7021 gccacgacgg cgtccatgcc caggcctgtc agaactttgt gatcgaagcc tgcgatttcc
     7081 gtactggcga tgattgtatt gccggatgtg acaatgagca ttttgttatc cgaaattgtc
     7141 aaatcaattc ttcgtgtaat ggttttcggt tgggatgtgt ccatctttta gtggagaatt
     7201 gccgattctg ggggccgggg gaatatcctc accgcattac ggttcggcag ggcaagccgc
     7261 ggacaaacat gctgtcggcc tttgtgcatt ttgcaccgac ggatcgtaat ccaaaactgc
     7321 ccagcgatga ctggcttgtc cgcaattgta cgatgacaaa tgttgaccgc gtttactctt
     7381 atgactttga aaagggtgtt tggcagacag gtcaaccggc caaacggcta gtattcgaga
     7441 atatccaggc cgagggaatc gcctttccat tgaatattct cggggataag gaccgccagt
     7501 ttgatctgac cctgcggaat gtggcgatca gccttcgtcc ggcttcggca cagcaacccg
     7561 tcattcaggc cagacaattt gggaaaatca ccttggaacg cgttgtctta aaaaacgatg
     7621 ggagccagcc ggtgcttcgt gcccgagaag gaaatgaatt gatcctccaa aacgtggcct
     7681 atcccccgga gtgttcccaa ccctttttat tggaggatat tgttcgcatc gaccgccgcc
     7741 cctaagagtg gagtgcgtct caaaaaggtt ccctctccct ccgggagagg gctggggtga
     7801 gggcaagcga tcgtcggaat cgattgagaa aaccgagagc gatacgagcg accctcaccc
     7861 ctgccccctc tcccgccgaa cgggcgaggg gggattatgg gataggctct aagttggcct
     7921 gggccgcttg atataaaggc gattgttcgg gtacccagag ggcgagactc aagaaacgac
     7981 catcccagta gatcatcaga tcgtcttcca gccactggct gagaagggcc ttcaggttct
     8041 ctggtgttat cgaccgggtg gccacaactt gagtaagcgt ttgcagcgtc tgggccgtat
     8101 cgcatgtgac gtacagctcg gcctccagcc cccgcaggcg gtgttgccgc atccggccac
     8161 cgcgcgtgtc aaatatgacc aactcgtcac gggttttcca gtgcattgtt aggtcgccac
     8221 tgccggagtc gttctgccag tgacggatgg cctctagaaa tggctcggcg aactcaagct
     8281 gcggttccca atcggcgtcg aattcgtagt agtaagccaa gcggttgagg acatcttggg
     8341 gaaagggata gacatgggca aacgcgcgag aagcacgagg gtttttcatc ccatagcgcc
     8401 caggactttc gaagaaaggc gaaaagcgat ccatgcgtgc ccgaccaacg cctaatggtg
     8461 acggaaaatg cggaatcttt gtgaccaaag acgcgagctg gcgatacgtg tcggctgttt
     8521 cccagggaaa gccatagagg atgttccatt ctgcggcgat gcgggcctcg gcaaaccact
     8581 tgagcgtctg aagattttga tatccacgga cgcctttgtt gaggtttttg agaatctccg
     8641 aatcgaacgt ctcaataccc aattgagccg cccgaagtcc tgcgcgtaga agttgctcga
     8701 tctgagaacg cttaagattg gttttcagtt cacaagcgaa agaaaacgct agctgagcct
     8761 tttccaactc gggcaatagc gaggcaaagt agcggaagtc caggatgttg tccgcggcac
     8821 aaccctgatg gacatcgtag cgggtgacga gatagcgtaa ttcatcgatc acgcgctggg
     8881 gggatttact gcgaaatcgt aacgactggc catttagccc acaaaacgcg cagtggtgtt
     8941 tttcacccca ccagcatccg cgggacatct caaagaaaag catcggggtc aattcgtgcc
     9001 gcaatggact ccgctggagc gtcgcgaagt agtcatcaaa atccgggtac ggtagggcgt
     9061 caaggttttc gaccaagacg gatcgcggtc ctcgcaaaat gtgcaattgt ggcagtatcg
     9121 gagatgccgg ttgacctgtg acggattcgc tttcggatgt gccctggagt gaagggccga
     9181 acatattcgc tcgggccacg acgccgggaa gaattggtgg tcttcggcca cctagcacgg
     9241 cctcggccag ttcgggaaat gtctcgtcgg cctcaccgag gaagacgtag tcgatttccg
     9301 gaaattggcg aaggagctca acgcccatct cgtcctcgca agcggccccg ccaaagacga
     9361 tgaccgtctc gggagacgcc ctcttgatcc gctgcgccag ggccaacgag gccaaagttt
     9421 gctgaaacgt ggtggcaaat cctacaagtg ccgcgtcctg ccaaggatag gcatcgaagg
     9481 ctgcgtcgat aaaccgctca atcttgggga agatggcaag atactcggcg tattcctcac
     9541 gtgtgagatc gcggtccgcc cggtgaagca cctcctcgta ataggcctgg tgatctgtgg
     9601 gcaactgtcc gaaatagtgt tgggagaaga ggcgttcgcc gccgaggaca aaggcataat
     9661 ggtcggcaat ccactcgtac aggtcataac cgatttcttc cgccaaagca aaattgagat
     9721 aaagcgtacg acacggcaaa tgccgcctct tcaaagcccc cgcaagcaag cccagcccca
     9781 aattcggcca gcgaaggtgg gcaaacggca tattgataag gacaatctcg cggctttccg
     9841 acataccaaa ctatacgaat aaaggatggg ttctcgttat cagagaccga tgtcttttat
     9901 ctgtttatcc gggaacctgg cacgaaatgc cgttcccaca gcatgaaacc tatctcgcgc
     9961 agcggtgacg gtgttagagc ctatcgcaca accgcccctc tcccgttcgg cgggagaggg
    10021 gccgggggtg agggtggctc ggatcgctgc tggttttctc gatcgactcc gatgatggct
    10081 tgccctcacc ccaaccctct cccagaggga gagggaattt tgggatgggc tcttaataca
    10141 ggacagtgac tcaattcccc gggcggttac aaacttcctc gctgatttta gcctttaggg
    10201 tactgacgac aatactcata cagcgcaatt gccgtcgctg tggctacatt atgggcgaac
    10261 ggcagtccgt aaacggggat ttccaccacg tggtccagga tggccaggat gtcgtcctcg
    10321 ataccgaggc gttcatggcc gacgacgagt acacagcggc ggggaaatgc aaactcataa
    10381 atccatatcg agccggtggt ctgttccagg ccgatcagcg ggaaaccctc ggcctttagc
    10441 tttttcagga ccggaaggag agagcgatga aattcgatct ggaggtctgg cccggtgtcc
    10501 cgcgcgatct ccggcaagat gcgggcatta ccacaacaga tgagacgacg aatcccacag
    10561 catcccgccg cccgaacaat gcgggaaaga ttaacgttac tcttcatcgg cgggcaggcc
    10621 acgatgagtt cccgcgggcg ttccagtgtg atgggttgtc gatggcgttg ctgtatgaat
    10681 cggctcatgg agaggacgtt gtcgcgggtg atgtcgaggt gttgggcgtt tgagaggtgt
    10741 tggctgactc gttcggaggc tgcacagggg ttgtcccggc cggagatggg acgagcgggg
    10801 ttttccatcc caataaggcc gagccgtggg tgatcccggc atccacatag cccctcagtc
    10861 gcctaagaag ccgtcgtgcc caagcgtatt ccaaacccaa gatggccagg ccaatgggga
    10921 tgacgagcgt tgccggccct ggggccacaa tcataatgat gcccaccaag actaccgtca
    10981 ggccgatgat ggtgatggcg atccgccgaa gccagcgaat gcccacccgc aaaaggatcc
    11041 ggtcaaacca tgtcaaactg gccaaaagtt cttccaattg cgccgctgct ccattctcag
    11101 gtgccgcatt gatttgtggc gtgtctgttc cacccaatcc ggcatgaagt ggcgtccaca
    11161 tggtgattta tccttttatc ttgccttttt atccagggct accggcgcga gtccttaggc
    11221 gaacgttgaa ctgaaaacat cccgatggct cggtcacagt cctgttaaga gcctatccca
    11281 caacctcccc tcgcccgttc ggcgggagag gggctggggg tgagggtggc tcgaatcgct
    11341 gctggtgttc cgatcggctc cgacgatggc ttgccctcac cctaaccctc tcccagaggg
    11401 agagggaatt ctaagatacg ctctaagtca atcatccttg cgagtgcgcc cgtaagcctc
    11461 actggctgtt gggcgattat cccgcttacc ccctggacat gccctttatt tatcataata
    11521 tccttgagtt ggctgttacc ggtacgccga ataccggatg ggctagagag ttcggtcgag
    11581 gagcaacgct gatggatgtt cagaatcttg ctcatgttcc cgcgtttatt accaaagatg
    11641 gctcggaaat ccgcgaactc ctctcatata ggaattcgtg cattcgcaac caatctttgg
    11701 ccgaggcccg cctgccccca gggcgaagta cgcagggaca ccatcacgtg cgatcggagg
    11761 aaatctatta catcctcgag ggcgaggcca ttgtgcaggt gggggaagaa aagcggcagg
    11821 ttggcccggg cgacgcagta gccattcccc cgggggcgta tcaccagatc accaacaccg
    11881 gtagcaccgt cctgcgattt ctctgttgct gtgccccgtg ctacgaacac gacgacacgg
    11941 tactgcggga ctaattcccg cactgcgcca cgggacccgc gggcgtgtcg cggcaaaaca
    12001 ccattgacta ctagaaaccg gtgacaaacg ccaccccggt tcctctggga tcagcgcggt
    12061 ggatcacttg gcaaccgcca cgcacatcgg ggcgaacggt tactccttga tccgcgcaag
    12121 ttcgttacaa aagcgttcga agcccatccg atag
//
LOCUS       DAOSUT010000127        12150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3030_length_12150_cov_8.963869, whole genome shotgun sequence.
ACCESSION   DAOSUT010000127 DAOSUT010000000
VERSION     DAOSUT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_12150_cov_8.963869"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(817..2220)
                     /locus_tag="PLD05_11535"
     CDS             complement(817..2220)
                     /locus_tag="PLD05_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOP78115.1"
                     /translation="MSRFLVSLLVLGAVLQGLVSNSDAAPPLLKVSPNGRYLVTDKGE
                     PFFYLGDTAWELFHRCTREEAELYLKDRASKGFTVIQAVVLAELDGLHTPNPYGHVPL
                     IDDDPTRPDIHPGSDNDYWDHVDWIVNRAEQLGLFIGMLPTWGDKWNKKWGVGPEIFT
                     PENARIYGEFLGKRYRDKPIIWILGGDRNPENERHLAIIRAMAAGLRTGDQGNHLMTY
                     HPQGGANSANWFHEDDWLAFNMFQSGHGRINNPNYLMTLANYERKPVKPTLDGEPRYE
                     DHPVNWRAEQGWFDDWDVRQGAYWSMLSGACGHTYGNHNIWQMYEPPRKPVSAARTPW
                     KKALHHPGSTQMGYMKQLFLLRPYYRLIPDQQLIAGQEKSEGEHRRAARAEDGTFAVI
                     YLPTGGSIRVITARLKGQNLHVSWFDPRIGKVVRSETVRKEAEITLAAPSAGRGNDWV
                     LVLDSDQENLPPIRIGE"
     gene            2422..3399
                     /locus_tag="PLD05_11540"
     CDS             2422..3399
                     /locus_tag="PLD05_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78116.1"
                     /translation="MSSDNLTVTCPHCGRQSRMPPGITGRTVRCPYCKETWKVPGGSQ
                     QASSISSSGPAGGVSGAERGGSPYEVSDPFALLSQTGGDVTAPVESTFSASTAPGMAT
                     APRTSAPPAWPMWWFLLGGLSVLLLISVVVAIVVVVVLFLQRSPQPAPPSQPPPVIAE
                     PAPPQPNPEDELARKGAATAAFLEAVFNESRKVLGDFSNLTNLGAGGASLPPDTPRRL
                     AAMEQALARLDTTDVDPVALQFKDRYVSEFKRLLNTMATTSQNLNAALQGGNPEAVLN
                     TAEQLGVAQSEYLKATSSFFEFLNTEGRRVKTDLESRYHRSFPMPLVPQ"
     gene            3517..4917
                     /locus_tag="PLD05_11545"
     CDS             3517..4917
                     /locus_tag="PLD05_11545"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOP78117.1"
                     /translation="MKRMRSCGGFLLLSVGLFFYAVLGPMVLGAEYYVDPQNGKPNND
                     GSKERPWPSLQALFDQGAIETQTWESLPYKPGVKLVPKNAGATIRPGDTIWLRTGNYG
                     RLFIQGYYNPDYITIAAEKGHTPRFESIHIQSGSHWILRGLHVSPEYGEGKRPRAMIR
                     LESHGWRGPVHDIIVEDCRLQSAEKTDHWSAEDWNNLSCDGILADGERIIVRRNYLKN
                     VNFGISMSGPHCLVEYNTIENFAGDGLRGLGDYGVFQYNVVKNCYDVNANHDDGFQSW
                     SVGPGGVGTGVVKGLVLRGNVIINYEDPQQPHRGALQGIGCFDGMYEDWVIENNVVIV
                     DHYHGITMGGAVRCRIVNNTVLDPTSLRPGPAAVRIGRHKNGTPSRDCIVRNNLVSAL
                     FVNGENMVVDHNLIVDDPAKFFVDPARYDLHLRKDAPAIDAGAADHAPERDIQGTRRP
                     QGKAVDIGAYEYPSEG"
     gene            5090..6388
                     /locus_tag="PLD05_11550"
     CDS             5090..6388
                     /locus_tag="PLD05_11550"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78118.1"
                     /translation="MMKSRREFLVEGSTVLSGLAVSNLLSRSVHAGHEAALKLGVIGV
                     GWYGMVDARAALQVGGVEIAAICDVDSEHLEKSASELEKLQGKRPKMFKLYEDMLSQT
                     DLDAVIIASPPHWHALHLWACLKHNLDVYCEKPLAYDIRECQAMVSAVQRSGRIVQVG
                     FQRRQHASFQQVRDFIASGEAGNIVQVDAQIHYRAGTKDPTPKDPPPSLDWNLWCGPG
                     PLIPYSEQVGHFNWRLEKTSGHGHLVDWGIHLIDATRMILGLTTPQKVVAAGGIYQLK
                     GIITTPDTLTVHFEFDRCPVVWRHRLWGAEEYSPDVNNGIFFYGTKATVFVTDGRWVV
                     VPAQKNSERKEYTPKTDAGVEHMRNFLTAVRERKQPACPIEEGYRSTATVKLAMIAYE
                     SDSIVRWDESQGDIQDNPTARALLKREYRAPWRHPADQEA"
     assembly_gap    6393..6492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6493..6951
                     /locus_tag="PLD05_11555"
     CDS             <6493..6951
                     /locus_tag="PLD05_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78119.1"
                     /translation="IDPRLLFVHNVHQAVEKEECEIVISPDDLLRRSLLPGVFFDAQK
                     EDMGTSRLTVTRYRCAASDWSLILYFYPAGVRRISPLCFEGRAAQNNQVLPQSCEEVW
                     AITTVSGRLEKGVTAVFHGGFLLVPPSMRPSELSQQIRDLVLNESPLFEG"
     gene            7162..8586
                     /gene="uxaC"
                     /locus_tag="PLD05_11560"
     CDS             7162..8586
                     /gene="uxaC"
                     /locus_tag="PLD05_11560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOP78120.1"
                     /translation="MADVFITENFMLENEMAVRLYHEVARDLPIVDYHSHLSPRLIAE
                     DYTFPNLTVLWLRGDHYKWRVMRANGVPEYFCTGNASDYEVFMKWAETVPKLLRNPLY
                     HWTHMELKKPFGISDRLLCPETAADIWRICNEKLTQPEFSCRGLLKQMNVVLVCTTDD
                     PTDDLKYHAQLAAETDFPVRVLPTFRPDRAMILANPESFHLWVDRLSEVSGVEVKDFS
                     TLIEALRKRHDYFHEMGCRVSDHGIEQFYAEDAKESELNQIFRDAYNGRLVSTNERAK
                     FKSAVLYHCAVMNYEKGWTQQFHVGALRNNNTRMFKQVGPDTGFDAINDVSMARNMVR
                     FFDRLDQEGKLTRTIVYDINPAFNEVFASILACFQDGSEPGKMQFGAAWWFLDQRDGI
                     LRQLETVSNFGLLSRFVGMLTDSRSFLSFVRHDYFRRILCNLLGSEMERGLLPKDFDL
                     VAKLVRDVCYNNAVRYFGFSLPLV"
     gene            8677..>12150
                     /locus_tag="PLD05_11565"
     CDS             8677..>12150
                     /locus_tag="PLD05_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78121.1"
                     /translation="MWKRLRHITLILWVGLIGWILTDSVGAYNPPVESASGITVRLEG
                     PSSVEGLTPFSVVLAVENSWEDSIKGSIKLGLIDGFTAEPAGPFAVEVPGKQVFRQEV
                     QIRPGEWVFNAHYPVHAWAIFEVDGVAVTLHPILIFEMKNVPQTKGPIWDWVPFAVPP
                     GGQMALEEIPIYRAVMQVFGQPPEVMPPGWRGSHSVHRGSVDVGSRTLGGIAKKGLVI
                     HPPWFEGKVGTALVEFPVTLPKVRPIQLRFSLGMQEEGQSDGVTFRVRVARHDAPAGE
                     FGAVVFERHTATKVWEEHEVDLSHFAGETIRLQLESHPGPKNNTGWDSSFWAEPTLVV
                     GGSEVTHVGNAESKRPITLGTTSLAGHPVIYEVVPGSRGLLDGLVRVRFDNREVAFQG
                     FEITVLGSRVDRPRSACGLIDWKLQVEPNPDVQGQSVTIRHRFNSFLGPFDLVGSLKV
                     VDGVLKSSFRLENIPQPQPWRHVYLQDVAFGGWTGRAFRVYAGPGNVVQNPQAYTLGF
                     DGHRLSTSFVGLEFKNGISVIQACDVPPTSFVVDPASNHSSLHASHHCEFTLVPAASV
                     WDGVKLWRERNGLRASPGVPQLAGRFVFDLWGGRYRSSADALQKAFKYGLTDSLVIWH
                     NWQRWGYDYRLPEIYPPNPHLGTLEDMQYLAKTCRDAGVLFALHDNYIDFYPDAEGFS
                     YLQTIAFSERREPVRAWFNEGRQAQSYRYRADQIEPFLKNNLVKIAENIGPTAYFIDV
                     WSSASPYDYWTAEGQFFDKIFTRKIWGEQFAWIRTLLGNQAPQISESGHDQLIGWLDG
                     ATTNHLRVGEPLPGYYRWSVWNWRCEDAERIPWFDAAHHDRFVLHGAGYSGRYQAGLN
                     PRLHGIYSDDYITTEVLTGHPAMVSEPFGVDVVRKYWLLAPLGRALALARIDNVEFVG
                     GDLHRIHVRWENGVETWCNRGASDWTIEGHVLPQYGFLARMPGHGASSNDAKGGQVIA
                     CICRRHGVITESVWGPEYVYVNGRKDYFGPERIRPKAMGFSSEGTTCEIKVTWLAPNG
                     VPEGYVPFAHFCDAEGEIVFQGRVSFIEGSSAEGEYQSVIRASLPDAIKPGTELELRV
                     GLYRPEDGRRLLLSGPDDGQSRIRLGKLTVAADRSLSWVGLPDEIDQLMLRRNSEGRE
                     IDFGGVITAQGL"
BASE COUNT         2589 a         3065 c         3546 g         2850 t
ORIGIN      
        1 gaggccgtgt tctcgaaagt gtaagttgcc gtacagaaga tcgggtcagt catggagtaa
       61 catggcttga cacgatcttc cccgccgtgc acacacaatg tggagaaacc gacagagtcc
      121 tttgacccct tcgaggcagt tggctcgttc gtcatagaag gtaccgcttt ctgttctgtt
      181 gttttgacta acgctaccca tgcaccgaca cagcggcccg aaccaactcg ctactcgcct
      241 gcttatgcta aagaaaaacc gcgacgaacg ggcgtcgcgg ctggcgagat tcgtgatgga
      301 tcgtcgcttt caaaccaacc tccaaagagc gacgatggca ctttagcaga tatggctgca
      361 agcggccgca aatcccattt ggccaaattc taccatcaac gacctgaatc gacaagaatc
      421 ggagaaattt ttggcgacgg tcgccttgtt tccgtccggc gcattgacct tggagactcc
      481 acactgcggt ggcccacttg cccaggtgta gggccacaca ccgtcgtggc cgcataactc
      541 gtcggcaagc cggttggtac gatgcggacc tacggccccg tcaattgaaa ttggggtccc
      601 aggcgatcga tgggcccaac ggggcgcgtc cctagaagtc tggcggaata cggcacgcgg
      661 catgaaaaaa acgaggcaac cgctggcggt tacccctaaa tcctcatgtg ttgcgggatc
      721 accaaccccc atcgcgaaac tggggttttg ttcggtgccg tccatagcga acaacggtag
      781 gcacaggcct tacgcctacg ccgcggtgct ccatagtcat tcgccaattc ttatcggagg
      841 aagattctcc tggtcactat ctagtacaag aacccagtcg ttgccacggc ctgccgaggg
      901 agccgccagg gtgatttcgg cttccttacg aacggtctcc gaacgaacca cctttccgat
      961 ccgaggatca aaccaagaga catgcagatt ctgccctttg aggcgagccg tgatcacgcg
     1021 gatcgaccct cctgtgggaa ggtaaataac ggcaaaggta ccatcctcag cacgcgcagc
     1081 ccgccggtgc tctccttcac tcttctcttg gcctgcgatc aactgttggt ctgggataag
     1141 acggtaataa gggcgaagga gaaacaactg tttcatgtat cccatttgtg ttgacccggg
     1201 atgatggagg gcctttttcc aaggcgtgcg ggcggcggac accggctttc gcgggggctc
     1261 atacatctgc cagatattgt ggttaccata ggtgtgacca caagcgccgg aaagcatcga
     1321 ccaatacgca ccctggcgga cgtcccaatc atcgaaccat ccctgttcag cccgccaatt
     1381 aacgggatgg tcctcgtagc ggggctcacc atcaagtgtc ggtttcacag gtttgcgttc
     1441 ataattggcc aaggtcatca agtaattggg attattgatg cgaccatgtc cagattgaaa
     1501 catgttgaag gcgagccaat catcttcgtg aaaccaattg gccgagttcg ccccaccctg
     1561 gggatgatac gtcattaggt gattgccttg atcaccggta cgaagccccg ccgccattgc
     1621 gcgaatgatg gccaggtgac gttcattctc cgggtttcga tcacccccca ggatccaaat
     1681 gatcggttta tcacgatatc gtttgccgag aaactcgccg tagatgcggg cattctccgg
     1741 cgtgaagatt tccggaccga caccccattt cttgttccac ttgtcgcccc acgtcggcaa
     1801 catcccgatg aataacccca attgctccgc ccgattgaca atccagtcca cgtgatccca
     1861 gtagtcgtta tcgctaccgg ggtgaatgtc gggtcgtgtg ggatcatcgt caatcagcgg
     1921 aacatgaccg taagggttcg gcgtgtgaag gccatccaat tctgccaata ctactgcctg
     1981 gatgactgta aagcctttgc ttgcgcggtc tttgaggtac agctcggctt cttctcgagt
     2041 acaccgatgg aaaagctccc aggccgtgtc tcccaaatag aagaacggtt ctcctttgtc
     2101 cgtcaccagg taacggccat tggggctaac tttgaggagc ggtggtgccg cgtcagagtt
     2161 ggataccaag ccttgtaata cggcgcctaa aactaatagg cttacgagaa agcggctcat
     2221 taatgttctc ccaacaaaac gcatttgaat cggtgggtgg cagtttcaag acgtttttct
     2281 ttgtctattt tagcgttagc tcctgggcat actagaatgg ccaggggatc ccttgcagat
     2341 ggtgcaaacg cctccgcctc acactatgat ggattgtcgg ctgagatgat ggcattcaac
     2401 caaaactttg ggggtttggc tatgtcttct gacaatttga cggtaacctg tccccactgt
     2461 ggccgacagt cgcggatgcc gccgggcatc acagggcgaa cggtacgttg cccctattgc
     2521 aaagagacat ggaaagtgcc gggagggtcc cagcaggcct cttcgatcag ctcatctgga
     2581 cctgctggag gagtttccgg agccgaaagg gggggttctc catatgaagt gtccgatcct
     2641 ttcgcgctat tgagccaaac ggggggagat gtaacggctc cggttgagag cacgttttct
     2701 gccagcacgg ctccggggat ggccaccgca ccgcggacca gtgcccctcc ggcttggccg
     2761 atgtggtggt ttctgctggg cggtctctcc gttcttctgt tgattagcgt ggtggttgct
     2821 atcgtcgttg tggttgtatt atttctgcaa agatcgccgc agccggcccc gccttcgcag
     2881 cctccgccgg tgatagcgga acctgcaccc ccgcagccga atccggaaga cgagttggct
     2941 cgcaaaggtg cagccactgc ggcatttctg gaggcggttt tcaacgaaag ccgcaaggtg
     3001 cttggtgatt tctcgaacct aaccaactta ggtgctggcg gagctagcct gccgccggat
     3061 actccgcgga gactggccgc tatggaacaa gccttggccc ggcttgatac cacggacgtc
     3121 gatcccgtcg cccttcaatt caaagatcgt tatgtgagcg aatttaagag actcctgaat
     3181 acgatggcca caaccagcca aaacttgaat gcggctctcc aagggggtaa tccggaggcc
     3241 gttttgaaca cggccgagca gttaggtgtg gctcagagtg agtatctcaa agccacaagt
     3301 agtttcttcg agtttctcaa taccgaaggt cgaagggtca agacggattt ggagagccgc
     3361 taccatcgca gtttcccgat gccgttggta cctcagtgaa aagacttgtg ccgcggtctg
     3421 gttaatcatt gccgattgcg gacaaccgac ttagggatag ggaactcgtg ctattttgag
     3481 tggctttggc gtgatgttga atgcgaggat ttaagaatga aaagaatgcg tagttgtggc
     3541 ggttttctgt tattgtctgt tgggttattt ttctacgctg ttttgggtcc gatggtcctg
     3601 ggggctgagt actacgtcga tccgcagaat ggaaaaccaa acaatgacgg aagcaaggag
     3661 cggccttggc cgagtcttca agcgttgttt gaccagggtg cgatcgaaac gcaaacctgg
     3721 gaatccctgc cttacaaacc tggtgttaag ttggttccca aaaatgcggg ggcaacgatt
     3781 cggccgggcg acacaatttg gctccgtacg ggtaactacg gtcgcctctt catccagggc
     3841 tattacaacc ccgactacat cacaattgcc gccgagaagg gacacacgcc ccggtttgag
     3901 agtattcaca tccaatcggg atcacattgg attttgaggg gcctgcacgt gagccccgag
     3961 tatggcgagg ggaagcgacc gagggccatg attcgccttg aatcgcatgg atggcgagga
     4021 ccggtccacg acatcattgt cgaagattgc cgtctccaat cagccgagaa gacggatcat
     4081 tggtctgcgg aggattggaa caatctctcc tgcgacggca ttttggctga cggggagcgc
     4141 atcatagtcc ggcggaatta cctgaagaat gtcaattttg gcatttccat gagtggccca
     4201 cattgcctgg ttgagtacaa caccatcgag aactttgccg gggatggtct tcggggattg
     4261 ggcgattatg gagtgtttca atacaacgtc gtcaagaact gctacgatgt gaacgcaaac
     4321 cacgatgatg gctttcagag ttggtccgtg gggccggggg gtgtgggcac aggggttgtc
     4381 aaggggctcg ttttgcgagg aaatgtcatt atcaactacg aagatcccca gcaacctcac
     4441 cggggagcgc ttcaaggcat tggctgtttt gatggcatgt atgaggattg ggttatcgag
     4501 aacaacgtgg tgatcgtgga tcactatcat gggatcacca tgggtggggc ggtccgctgc
     4561 cggattgtca acaatacggt ccttgatccc acgtcgttac gtcccgggcc ggctgccgtg
     4621 aggattggcc gtcacaagaa cggaacgccc tcccgtgatt gcatcgtacg taacaatctc
     4681 gtgtcggccc tgtttgtgaa cggtgaaaac atggtcgtcg atcataatct tatcgttgac
     4741 gatccggcga agttcttcgt cgatccagcg cggtatgatc tccatttgcg aaaggacgca
     4801 ccggccattg atgccggcgc cgctgatcac gctcccgagc gagatattca gggaacgcgg
     4861 cgaccgcagg gaaaggccgt ggatattggt gcttatgagt atccgtccga gggttagccg
     4921 gcggtcagat tagcgtgact gcccaaaaac gactggttgc aagaacacga caaaacagtc
     4981 tgtgttcagt ggaaccgact gtggtttgcc tcagggtctt aggtcatata attacgtgca
     5041 ttgagtgagc aagttccgtt ttgcaggtac gtcacgagga gacgccaaca tgatgaagtc
     5101 ccgtcgcgaa ttcttggtgg agggttcaac cgttttgtct ggcttggccg tttccaattt
     5161 gctcagccgt tcggtccatg ccgggcatga agcggcgtta aagctcggtg ttatcggtgt
     5221 aggttggtac gggatggtgg atgcgcgggc ggcgctgcaa gttggtggtg tggaaattgc
     5281 cgcgatttgc gatgtcgatt cagaacatct tgaaaaatct gcgagcgagt tggaaaagct
     5341 ccagggtaag cgtcctaaga tgttcaagct ttatgaggat atgctcagtc agaccgacct
     5401 cgatgccgtg attattgcca gccctcccca ttggcacgcc ttgcatttgt gggcatgtct
     5461 caagcataac ctcgatgtct attgcgaaaa gccgctggcg tacgatatca gggagtgcca
     5521 ggcgatggtc agcgcggtgc aacggtcggg gcggattgtc caagtggggt ttcaacgccg
     5581 tcagcatgca agctttcagc aagtccgtga ctttatcgcc agcggtgagg cgggcaacat
     5641 tgttcaggtc gatgctcaaa ttcattatcg tgctggaacc aaagatccaa caccgaaaga
     5701 tccaccacca agccttgact ggaatttgtg gtgtgggccg ggaccgctca ttccgtacag
     5761 cgaacaggtc ggccatttca actggcggtt ggaaaagaca tcagggcatg gccatcttgt
     5821 tgattggggg attcacctga tcgatgccac gcggatgatc ttggggctaa cgacacctca
     5881 aaaggtagtg gcagctggag ggatttatca gctcaagggg attatcacca ctccagacac
     5941 cttgaccgtt catttcgaat ttgatcgatg ccccgtggtt tggcggcacc gattgtgggg
     6001 ggccgaggag tactcacccg atgtcaataa tgggattttc ttttacggaa ccaaggccac
     6061 ggtatttgta accgacggtc ggtgggtggt cgtcccggcg cagaagaaca gcgagcgtaa
     6121 agaatatacg ccgaagaccg acgccggcgt tgaacatatg cggaacttcc taaccgccgt
     6181 ccgagaacgg aagcagccgg cctgccccat tgaagaaggc taccgctcga cggccacggt
     6241 caagttggcg atgattgctt acgagtcgga cagcattgtg cgttgggatg agtcgcaagg
     6301 ggatattcag gataatccaa ctgctcgggc gttgctgaaa cgggaatacc gcgccccttg
     6361 gcgacatcct gccgaccaag aggcgtagtc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnatcgaccc tcgtcttcta tttgtccaca acgttcatca agctgtggaa
     6541 aaagaggaat gtgagatcgt tatctcgcca gatgaccttc tgcggcgttc actcttgcca
     6601 ggggtgtttt ttgacgcgca gaaagaggac atgggcacgt ctagattgac tgtcacgcga
     6661 taccgttgtg cggcctcgga ctggtcactg atcctctatt tttaccccgc gggcgtgcgg
     6721 aggatatcgc cgctttgttt cgaaggtcgt gctgcccaaa acaaccaagt actgccgcaa
     6781 tcgtgcgaag aggtctgggc tatcacgacc gtttcggggc ggctcgaaaa aggtgtcaca
     6841 gcagtttttc acggtggctt tcttttggtg ccaccgtcaa tgcgaccatc agagctttcc
     6901 cagcaaatcc gcgatcttgt gctaaatgaa tccccactgt ttgagggata acctggaatt
     6961 tctcggcatc tcccggtagc atgggatcgc attgttttgc gtcgagagaa cgcacagccg
     7021 ttttgctgat tgggtggata cttgctgctt gctggcaatc ggtgggcagc tttagggggt
     7081 gggagtgcaa atctgaagtc tcggggcaag caggaaatta acggtatcag gtttgagttt
     7141 ccttggtaga aggagaacga tgtggctgac gtgttcataa ccgaaaactt catgctggaa
     7201 aacgaaatgg ccgtcaggct ttaccatgaa gttgcgcggg accttccgat tgtggactat
     7261 cattcccact tgtcaccgcg gttgattgcc gaggattaca cattccctaa tttgacagtg
     7321 ctatggcttc gtggtgacca ctacaagtgg cgcgtcatgc gggcaaacgg cgtccccgaa
     7381 tacttttgca cgggcaatgc aagcgattat gaggtgttca tgaagtgggc agagaccgtc
     7441 ccaaaactgc tccgcaatcc gctttaccat tggacccaca tggagctgaa aaagcccttc
     7501 ggaataagcg atcggcttct ctgcccggag acggccgccg atatctggcg gatttgtaat
     7561 gaaaagctaa ctcagcctga gttttcctgt cggggccttc tcaagcagat gaatgttgtg
     7621 ctggtctgca cgactgatga tccgacggat gatctcaagt accatgccca attagcggcc
     7681 gagacggatt ttccggtccg cgtgctgccg acatttcgac cggaccgggc gatgatcttg
     7741 gccaacccgg agagtttcca tttgtgggtt gatcggttga gtgaagtctc cggcgtggag
     7801 gtgaaggatt tttccacgct gatcgaagcc ctccgtaagc ggcatgatta tttccacgag
     7861 atgggttgtc gtgtttcgga tcacggcatt gagcagtttt atgccgaaga tgcgaaagag
     7921 tccgagttga atcaaatctt tcgtgacgct tataacggcc gcttggtcag cacgaatgag
     7981 cgggccaaat tcaagtcggc cgtgctgtat cattgtgccg tcatgaacta cgagaagggg
     8041 tggacgcagc aattccacgt cggggccttg cggaacaaca acacacggat gttcaaacag
     8101 gttggaccgg ataccgggtt tgacgccatc aatgacgtca gcatggcccg aaatatggtg
     8161 cggttcttcg atcgtctcga tcaggaaggg aaactgacaa ggacgattgt ctatgatatc
     8221 aatccggctt tcaatgaggt cttcgccagc attttggcgt gctttcagga cggttctgag
     8281 ccgggaaaga tgcaatttgg ggcggcatgg tggtttcttg atcagcgaga tggtattctc
     8341 cgccaattag agacggtttc caatttcggc ctgctcagcc gcttcgtggg gatgctgacc
     8401 gacagccggt cgttcctctc gtttgtccgc cacgactatt tccgaaggat cctttgcaat
     8461 ctcttgggga gtgaaatgga acgcggcttg ttacccaaag acttcgatct tgtggcaaaa
     8521 ctcgtacgag acgtttgcta caataacgcc gtgcgatact ttggattctc gttgccactt
     8581 gtgtgaggct tgtaacccgg cgactcggaa gcgggtaggt tttcggtgtc gctgcaatcg
     8641 ggggtgtacc tgcggagtcc acgggagggt caaccaatgt ggaagagatt aagacacata
     8701 acgttaatcc tttgggtcgg tttgatcggc tggattttga ctgactcggt tggtgcctac
     8761 aatccacctg tggagtctgc ctcgggaatc acagttcggc ttgagggacc gagcagtgtg
     8821 gaaggcttga cgccgttttc ggtggtactg gcagtggaaa acagctggga ggactctatt
     8881 aaggggtcta tcaagctggg actgattgat ggttttacag ccgagccggc ggggcctttc
     8941 gctgtggaag tacccggcaa acaggtcttt cggcaggaag tccagattcg tcctggggaa
     9001 tgggtcttca acgcccatta tccggtgcac gcttgggcga tcttcgaggt ggacggagtt
     9061 gccgtcacgt tgcatcccat ccttatcttt gaaatgaaaa acgttcccca gacaaagggg
     9121 ccgatttggg attgggtacc ttttgccgtc ccgccgggcg gacaaatggc gttggaggaa
     9181 atcccgattt atcgggccgt aatgcaggtt tttgggcaac cgcccgaggt gatgcccccc
     9241 ggatggcgcg gatcccattc ggtgcaccgt ggttcggtcg atgtgggctc tcggacactc
     9301 ggcggcattg ccaaaaaggg tttggtcatt catccgccct ggttcgaagg caaagtaggg
     9361 accgccctgg tcgaatttcc ggtgacgctc cccaaagtgc ggccgatcca gttacgattc
     9421 tcccttggaa tgcaggagga ggggcaaagt gatggtgtga cgttccgtgt ccgtgtggca
     9481 aggcacgatg cgccggcggg ggagttcggt gctgttgtct tcgagcgtca cacggcgacc
     9541 aaggtgtggg aagagcacga ggtggatctc tcccactttg ccggggaaac gatccgtttg
     9601 cagttggagt cccatccggg acccaagaac aatactggct gggattcttc cttttgggca
     9661 gagccaacac ttgtcgtcgg tggaagtgag gtaacacacg tcgggaacgc ggagtcgaag
     9721 cgccccatca ctttggggac cacttctctg gcaggtcatc cggtgattta tgaagttgtg
     9781 ccgggatcgc gaggcctact cgatggcctt gtccgagttc gattcgacaa cagagaggtc
     9841 gcgttccagg ggtttgagat tactgtgtta ggatcgcgcg tggaccgccc gcgttccgcc
     9901 tgcggcttga tcgattggaa gttacaggtc gaaccaaatc ccgatgttca ggggcaaagt
     9961 gtgaccattc gacatcggtt caatagtttc ctcggtccct ttgatctagt cgggtcctta
    10021 aaggttgtcg acggtgtttt aaaaagcagt tttcgcctcg agaacatccc tcagccgcag
    10081 ccctggcgtc acgtgtattt gcaagatgtc gcattcgggg gatggaccgg gcgtgcattt
    10141 cgcgtttacg ctggcccagg gaatgtcgtc cagaatcccc aagcctacac tttgggtttt
    10201 gacggccatc gattgtccac gtcattcgtg gggctggagt ttaagaatgg tatctcggtg
    10261 atccaggctt gcgacgttcc gccaacgtcg tttgtcgttg acccggcatc taaccacagt
    10321 tcgctgcacg cttcgcatca ttgtgagttt accttagtcc ctgccgcatc ggtgtgggat
    10381 ggcgtcaaac tgtggaggga aagaaacggt ttgcgggcgt ccccgggcgt tccacaactg
    10441 gccggacggt ttgtctttga cttgtgggga gggcgatacc ggagtagtgc cgacgccctg
    10501 cagaaggcct tcaagtacgg tctgacagac agcctggtca tttggcataa ctggcagcga
    10561 tggggctatg actaccgctt gcccgaaatc tatcccccca atccgcacct cggaacccta
    10621 gaggatatgc aatatctcgc taaaacgtgc cgtgatgcgg gggtcctttt tgccctgcac
    10681 gacaattaca tcgacttcta cccggatgcg gaaggctttt cctacttgca gaccattgcc
    10741 ttttctgaga ggagagaacc cgtgcgggcg tggttcaacg aaggccgcca ggcccaatcc
    10801 taccgctatc gcgcggatca gattgagccg ttcctgaaaa acaacctggt taaaattgct
    10861 gagaatattg gccccacggc atatttcatc gacgtgtgga gtagtgcaag tccctatgat
    10921 tactggaccg ccgagggcca attcttcgac aaaatcttta cccggaaaat ttggggagaa
    10981 caatttgcct ggattcgaac acttctcggc aatcaagccc cacagatatc cgagagtggc
    11041 cacgaccaat tgattggttg gcttgatggt gccacgacga accatctgcg ggttggggag
    11101 ccactgcccg gttactatcg ctggtctgtt tggaactggc ggtgtgaaga tgccgagcgc
    11161 atcccgtggt ttgatgcggc ccaccatgac cggttcgttc ttcacggtgc cggatattcc
    11221 gggaggtacc aagcgggttt gaaccctcgt ttgcatggaa tttacagcga cgattacatc
    11281 acgactgagg tcttgacggg ccaccccgcc atggtttccg agccatttgg tgtggatgtg
    11341 gtgcgaaaat actggctcct ggctccgttg gggcgcgccc ttgccctggc gaggatcgac
    11401 aatgtggaat tcgtcggcgg cgatctgcac cgcatccacg tgcggtggga gaacggagtg
    11461 gaaacctggt gcaatcgggg agcgtcggac tggactatcg aagggcacgt tttgccccag
    11521 tatggatttc tcgcccgcat gcctgggcac ggggcgtctt ctaatgacgc taagggtggc
    11581 caagtgatcg catgtatctg caggcgtcat ggtgtgatta cggaatctgt ttgggggcca
    11641 gaatacgtgt atgtaaacgg tcgaaaggac tacttcggtc ccgagcgaat tcgcccaaaa
    11701 gcaatggggt tttcctcaga agggactacc tgtgagatca aggtgacctg gctggctccc
    11761 aacggcgtcc cggagggcta tgttcccttt gcccattttt gcgatgccga gggtgagatc
    11821 gtctttcaag ggagggtgtc attcatcgaa gggagctctg ccgagggcga ataccaaagc
    11881 gtcattcggg caagtctccc ggacgccatt aaacccggta cggaattaga attgcgcgtg
    11941 ggactctatc gacccgagga tgggcgtcga ctcctgctga gcggtcctga cgatggtcag
    12001 tccaggatcc gtttggggaa actgacggta gcggcggatc ggtccctgtc ctgggttggc
    12061 ctgccggacg agatcgacca gcttatgttg cggaggaatt cggaaggccg agagatcgat
    12121 ttcgggggcg taattacggc ccaggggctg
//
LOCUS       DAOSUT010000128        11730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3115_length_11730_cov_9.340043, whole genome shotgun sequence.
ACCESSION   DAOSUT010000128 DAOSUT010000000
VERSION     DAOSUT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_11730_cov_9.340043"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLD05_11570"
     CDS             complement(<1..352)
                     /locus_tag="PLD05_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78122.1"
                     /translation="MRWLVEDPTIPIVAIVIVEAVLAAFFVSTLKKAFLWAMVGMVVP
                     LASILLADYLVVTDREAIETTIRKGVSALQANDLNQVLTFLSPSAVKTRERAEWAMSL
                     VTFTSVRISDLQIQV"
     gene            860..1720
                     /gene="surE"
                     /locus_tag="PLD05_11575"
     CDS             860..1720
                     /gene="surE"
                     /locus_tag="PLD05_11575"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOP78123.1"
                     /translation="MLILLTNDDGIYSPGLIALYEALQELGEVCVVAPATEQSGVGHS
                     ISYLRPLILKQVFHGDQHFGFAVDGSPADCVKLGVSELCPRRPDLVVSGINMGLNAGI
                     AVLYSGTVAAAIEGAFFGILSVAVSLQFDDNPQFSRAARLAVQIIQQILKQRSDEVRL
                     FNLNIPTKAVMDESPKVRVVSMDIRQYGEKYEKRVDPWGRTYYWATGEPSPTPEDRAT
                     DLSVLARGEIALTPLHYDLTAKSVLSKISEWEFPIRAEDVGSNVPGIPASVVVRTTHR
                     LQSVIPSKDQ"
     gene            1746..2405
                     /locus_tag="PLD05_11580"
     CDS             1746..2405
                     /locus_tag="PLD05_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78124.1"
                     /translation="MARCHRNRLFFGALAVMVVLMPLLLGCSQDSADSFRVKQSSPTA
                     STPPAPPPPPSASQPAPPASEPQRRPVAMPPGIARPEDVTSDFSSPAPAQPSGGMTPP
                     PPTSPQALPAATQGQYVRADVGVGQKGRTLERGFITTPVQVYFRSREHMAFRIQVPQA
                     LQLFQGEHGRLPESHEEFWREIIEKNGIQLPSLPPGHRYVYVPEKGELMVEVPAGSPP
                     P"
     gene            3307..4005
                     /locus_tag="PLD05_11585"
     CDS             3307..4005
                     /locus_tag="PLD05_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78125.1"
                     /translation="MGDNDQKQDQPKPPPPPPPVLNQVILAVIALGAILSPVLLLYGS
                     GHIADGVARILSGEPIVAALGVRPATAPQDKPAAAGGENTFELQPVDFGEVFRFDITP
                     DWIVTRWPRVTASLSAIQLQGYRVPLVTGIAEDDLAGTVTYYFNASRQLQRITFEGTT
                     GTPNRLLAFLSTYYGFVYRPTNNPSVYLFVVPEEGSRGETHSYVWIRPAPVFQANERH
                     HRFDVSVVIERPTR"
     gene            complement(4143..5156)
                     /locus_tag="PLD05_11590"
     CDS             complement(4143..5156)
                     /locus_tag="PLD05_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOP78126.1"
                     /translation="MIKDETTSPPADCVKHAVSPPPTTDVLRLTENVKLLLEHGVQVA
                     KATKAAAIALLWPGKAEWTRVRNLVQDHKLIVVVDTQAQAKAPQEAGLLTVALNLSAA
                     SPMERLSQTLVECVSKDLVSVTDDLVVIYGAFDAGTLDSLGVFKLSDYLGRLTIRELR
                     QLQTDIPLETLKTVINLAIEIGREGREGKPVGTMFVVGDTRKVLAHTHPLGFDPVRGY
                     SKRERNLFDPRVREGIKEVAQLDGAFIISKDGEVVAACRHIDCSADGINLSKGLGSRH
                     WAAAAITRRTDAIAVCVSESSGTVRVFQNGEVRMRIEPEMKRPVVWKESDFDGAIHLD
                     ERR"
     gene            5611..>5909
                     /locus_tag="PLD05_11595"
     CDS             5611..>5909
                     /locus_tag="PLD05_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrBC"
                     /protein_id="HOP78127.1"
                     /translation="MKCQKCDRPATYHITELTGPTPVEVHLCEQHAQEYLGRGSEPSV
                     EMTQVAEQMAQQLAKHMVVGQAAEEMKRLDQQACPYCGITFYKFRNQGRLGCPHD"
     assembly_gap    5910..6009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6010..7089
                     /locus_tag="PLD05_11600"
     CDS             <6010..7089
                     /locus_tag="PLD05_11600"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOP78128.1"
                     /translation="TSSTMLTVDDLMQGCGEWLQGKGRDNDIVISTRIRLARNLADFP
                     FTSIASEAEQRQILDRVRDVIAASPFSEQLLYVPMEELSELDRQFLVERHLISRELAE
                     GQGPKAVFIDHAERFSIMVNEEDHLRIQAMQSGFEPEPTWALINELDDYLGQKLVFAY
                     HQEWGFLTACPTNVGTGMRVSVMLHLPASVIVKQIEQIFRSLEKLELVVRGLYGEGSQ
                     ALGDFYQVSNQVTLGRSEGELIRKMLDVIPAIVGAERKARQFLLDEGSQRVHDRISRA
                     YGVLKTAQIISSQETLECLSLVRMGVHLGLVKDISIPLLNELFLYTQPAHLQKVVGVE
                     MDSADRNVERARFLKKKLGTAGPMG"
     gene            complement(7141..8580)
                     /gene="purB"
                     /locus_tag="PLD05_11605"
     CDS             complement(7141..8580)
                     /gene="purB"
                     /locus_tag="PLD05_11605"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOP78129.1"
                     /translation="MSKSDPYAMYDNPLVGRYASQEMSELFGPRRRIETWRRLWVILA
                     ESQAELGLPITEQQIAEMKSQISNIDWEKAAEYERRFRHDVMAHVHAFGDVCPSARGI
                     IHLGATSCYVTDNTDLILMREGLKLIARRLAAAIDHLGTFAAKYRDLPCLSFTHFQPA
                     QPTTVGRRACLWAYDFAMDLQEVEHRLSQLQARGVQGTTGTQASFLTLFNGDHEKVKK
                     LNQLVCQKMGFPGSYVITGQTYSRKIDAQILATLAQIAATAHKVATDIRLLAHRKEIE
                     EPFEKEQIGSSAMAYKRNPMRSERICGLARYVMSLEANTTLTHGVQWLERTLDDSANR
                     RLVIPQAFLAIDAILILCCNVFSGLVVYPKVIEKNLLAELPFMMTEDILMAAVQAGGD
                     RQELHERIRQHSHQVARKIKEEGGENDLIERLKADPAFAEVNFESVTDPLKYVGRSRE
                     QVDEFIRDVVQPIRERYTGARDLAAEVQV"
     gene            complement(8636..10039)
                     /locus_tag="PLD05_11610"
     CDS             complement(8636..10039)
                     /locus_tag="PLD05_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP78130.1"
                     /translation="MRPDYDQESLSHDPIHGYIPFVSRRGVSTGEVSEQELIDHPWIQ
                     RLRQIHQLQTAWWVFPSATHTRFQHVLGVMHLAGRIANELYPTLAASDPNVPSRGYVV
                     SLIRVAGLLHDVGHGPFGHFFDQFYLSRYQLNHESLGAEIIRRDLAPLIRGIRRNPFG
                     KLESHEELDPEQVCFLMMRPRSGSDRTAPLWLRLLRGLFSGIYTVDNMDFVLRDAYMS
                     GYSQKAFDLERLIHYSFFTPQGVTIHERGLSALVRFITVRAELFRSIYFHRTVRSIDL
                     SLQDLFQESANLLFPGNPLDHLEEYRRLTDWSLLVDVERWAESSDERRARLGHQWREW
                     LARKIRWKMVCERTIFFGPGKAEEMSVFSDEHLFEEAVRRRLPSDLRDMPLRFDPPRH
                     VHRPGTRAPAAGQNFLYDPAQNAVRSLDARELYRQIPLSYRICRVYALSTEHASEIAQ
                     AMDSLLSEDHVDDVTNM"
     gene            10244..10948
                     /locus_tag="PLD05_11615"
     CDS             10244..10948
                     /locus_tag="PLD05_11615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOP78131.1"
                     /translation="MYRAIGLGVLLMLLPTLSVSAQPPQRTPPSGGPGARGTTAFEVK
                     TLPKDDIEKQILDVIAEVGANQRAGNMIVPEEDGRLLRAWAEAVGAKHVVEIGTSVGY
                     SGLWFCLVLKKTGGKLTTFEIDERRAAQAKKNFERAGVSDIVTIVLGDAHQTVTQLKE
                     PIDVLFLDADKTGYIDYFEKLSPLVRPGGVVIAHNMTPRSADPRFVKAITTTPEYETV
                     FLHMEGAGVSVSVKKH"
     gene            complement(11213..11560)
                     /locus_tag="PLD05_11620"
     CDS             complement(11213..11560)
                     /locus_tag="PLD05_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOP78132.1"
                     /translation="MKLIIAIIQPHRLEAVKQALADVEVFRLTVMDVQGFGRERGHAE
                     AFRGHEFSVNLTRKVQLMIAVNDNFLEPTIDAIIKGARTGPQGQVGDGKIFVLPLEDC
                     IRIRTGERGGEAI"
BASE COUNT         2579 a         3072 c         3303 g         2676 t
ORIGIN      
        1 tgacctgaat ttggagatct gaaattcgga cactggtgaa ggtgacaagg gacatggccc
       61 actccgccct ctccctagtc ttaacagccg agggggatag aaacgttaaa acctggttta
      121 agtcgttggc ctgcagagca ctcacgcctt ttcgaatcgt cgtttcaatg gcctcccgat
      181 cggtgacgac gaggtagtca gccaacaaga tacttgcaag cggaacgacc attcccacca
      241 tggcccaaag gaatgccttc ttgagggtcg atacaaaaaa cgccgcgagg accgcttcga
      301 cgatgacaat ggccacgatg gggatagtgg ggtcctcgac aagccaacgc ataaatggta
      361 ctccacgcag actggaccac ttaagagccc atcccataat ctcccctcac gcgttcggcg
      421 ggagaggggt cggggatgat ggtacctcga atcgttgccg gttttctcga ttaatttcga
      481 cgttcgcttg ccctcacccc aaccctctcc cgaagggaga gggcgacttt ttgggatagc
      541 ctctaagtct aaaaggcgat ctctcgcttg caactcgttg tcttgatcct acagttttgt
      601 cggagttggg gcaatgaaga gcacggagat ttgttttctg ggcataaggt tgagctataa
      661 gcgagaattc gatgtcgttt agccaagcca tttgaagtct tccaaacctt tagcaagcgc
      721 cgtgatttta cggctaattg gttgccgctg gggttaccgc cggatatagt gaaaaggtaa
      781 tcgaagttta tattggcttg atagccgcag acaaggtgcc aagttctttt tcccgtttct
      841 ggggagactg gtcaataccg tgttaatcct tttgacgaat gacgacggga tttattcgcc
      901 gggcttgatc gccctttacg aggcactgca agagttaggc gaggtctgtg tagttgcacc
      961 ggccactgag caaagcggtg tgggccattc catcagctat ttgcggccgt tgattctgaa
     1021 acaggtattc cacggtgacc agcattttgg tttcgccgtc gatggaagcc cggcggattg
     1081 tgtcaaatta ggggttagcg agctttgtcc ccgtcgtcct gatcttgtcg tcagcggtat
     1141 caatatgggc ctcaatgcgg ggatcgccgt gctttattct ggaacagtgg cagctgctat
     1201 cgaaggggcc tttttcggca tcttgagcgt ggccgtatcc ttgcagtttg atgacaatcc
     1261 gcagttttcg cgggctgccc ggctggcggt acagattatt caacaaatcc ttaaacagcg
     1321 gtcggatgag gtgcggcttt tcaatctcaa tattcctact aaggccgtca tggacgagtc
     1381 gcccaaggtg cgcgttgttt ccatggatat tcgccagtat ggggagaaat acgaaaagcg
     1441 cgtcgaccct tggggaagaa cctattattg ggcgacgggg gaaccgagtc ccacgcctga
     1501 ggacagagcg accgatttgt cggttctggc acgaggagag atcgccctta caccgcttca
     1561 ctatgatctg acagcgaaga gcgtgctttc caagatatca gagtgggaat tcccaattcg
     1621 tgctgaggac gtgggatcaa acgtaccggg cattcccgca tcggtggttg tgcgaacgac
     1681 gcatcgtttg caatcggtga taccgtcaaa ggaccagtga ggattcacag gtacaggaga
     1741 tcgccatggc tcgatgtcat cgtaatcgcc tgtttttcgg cgctctcgcc gttatggttg
     1801 tcttgatgcc gttgttgttg ggatgctctc aggactcggc ggatagtttt cgggtcaaac
     1861 aaagttcgcc aacggcgagt acgccgccgg ctcctccacc gcccccttcc gccagtcaac
     1921 cggctcctcc ggcaagtgag ccgcagcgtc ggcccgtggc catgccgcct ggtatagcgc
     1981 gtcccgagga cgtcacgtcc gatttctcgt cgccggcccc ggcacagccg tcagggggaa
     2041 tgacgcctcc cccaccgaca agtccccaag cacttccggc ggccacccag gggcagtatg
     2101 ttcgggcaga cgtaggagtt gggcagaaag gccggacttt ggaaagggga ttcatcacca
     2161 cgccggtgca agtctatttc cgtagtcgag aacatatggc atttcgcatc caggtccccc
     2221 aggccctcca gctcttccaa ggagaacatg gccgcctgcc cgaatcgcac gaagagtttt
     2281 ggcgcgagat tatcgaaaaa aatggcatcc agctgcctag cctcccacca gggcatcgat
     2341 atgtctatgt cccggaaaaa ggggagctga tggttgaggt tccggctggt tcacctcccc
     2401 cataaagagg ggttaattag cacccggggg catcctcaca tgaggagctt gagacgtgac
     2461 ggaagttgcc tttcgggatg gactcgcctt gaaaactctt cgcctgcaat taacctcgat
     2521 gatggggccg ttgattgtga tctgaggtcg cgggcggcgt ttttgcgagt tcgagacttt
     2581 catggtgtct tctctcggca aaccttctct tcagaacctt ctgaggcttt ggaccctaca
     2641 tgtcctcgcg gcaatgctgc taggtccgct tgattcacca tgcttcggcg agagttggat
     2701 tttccagcct tcctattttt ctcatcagga gaagggggaa cgggtggcgc aatacgcccc
     2761 acctctcccg tccgttgccc cttacgcgcc aaattatgta gtcagtggct atcgacatag
     2821 caaaagcacg atccgatcag cggaaagcta tgatcatgta catatcgtga aaacttgggg
     2881 tttgggggct tacattcgtc cttatgagga atgggagtac ccgttccggg aaggggctac
     2941 cccattcggg ccatggggca atccgcaagg tccgtggacg ctgccgtttc agtcctggat
     3001 caacccattt gccctcggcc gtttgccgaa ccctccctgg tggatttatc ccccctattg
     3061 gccaccacca ccgcgcccgt gaaactttgt tgggggtgcc aggagatcta cgcgtgatat
     3121 aaaacgtcgc ttctggcgaa ttgagcaacc caggttctgt tggcgaaagt tttttcgtag
     3181 gtaacatttc aaacaaacct gacggttggc gaagccatct tgcagcgcgg ggcccgtttc
     3241 gcccgaaggc aatgaaagga ggcaattgtt tgagcagtct tcctttccgc caaatgacct
     3301 gaaccgatgg gcgacaacga ccagaagcaa gaccaaccga aaccgccgcc cccaccaccg
     3361 ccggttttaa accaggtcat ccttgccgtc attgcgttgg gggccatcct ttcgcccgtc
     3421 ctgcttttgt atggttcggg gcacattgca gatggggtgg cacgcatcct ttcgggcgag
     3481 ccaatcgtcg ccgcactggg agtcaggcca gcaacggccc cccaggataa accagcggct
     3541 gccgggggag aaaacacttt tgagctacag ccggttgact ttggagaggt cttccggttt
     3601 gatatcactc cggactggat tgtgacgcgc tggccgaggg tcacagcgag tttgtcggcg
     3661 attcaattgc agggatatcg cgttccattg gtcacgggca tagccgagga cgatttggcg
     3721 ggaacggtga cctactattt caacgcgtca cggcaacttc agcggattac cttcgaaggc
     3781 acgaccggca caccgaatcg acttctcgcc ttcttgagca catactacgg gtttgtctat
     3841 cggcccacca ataatccaag cgtgtatttg ttcgtggtgc cagaggaagg ttctcgtggc
     3901 gaaacgcaca gttacgtgtg gattcgccca gcgccggttt ttcaggccaa cgaacggcat
     3961 catcggttcg acgtctcggt agttattgaa cgccctacca ggtgaaagaa acaggggcat
     4021 ttacgccgaa caaccgaccg gcgtcatatc taccgtggca gaatcggtaa gtgttccggt
     4081 tttgtgggcg ttctcttgca gcgccaggat tgcagctgcc gtacgcagtc aggttagcgc
     4141 cgtcaacgcc gctcgtcgag atggatggcg ccgtcgaaat ctgattcttt ccacacgacg
     4201 gggcgtttca tttccggctc aatgcgcatc cggacttcgc cgttttggaa aaccctcacc
     4261 gttccgcttg actcgcttac gcatacagcg atagcgtcgg tacggcgagt gatcgcggcg
     4321 gccgcccagt gccgggatcc tagaccttta ctcaaattga ttccgtctgc ggaacaatcg
     4381 atatggcgac aggcggccac gacttcacca tccttgctaa tgatgaaggc tccgtccaat
     4441 tgggcaacct ccttaatccc ttcccgcacc cgggggtcaa aaaggtttcg ctcccgctta
     4501 ctgtaaccac gaacggggtc aaaacccaag ggatgagtat gagccaagac tttccgggta
     4561 tcacccacca cgaacatagt gccaacgggt ttgccctcgc ggccttctcg tccgatttcg
     4621 atggcgagat tgatcacagt ctttagcgtt tctaagggaa tgtccgtctg gagttggcga
     4681 agttcccgaa tcgtcagtcg ccccaggtaa tctgagagtt taaacactcc caaagaatcg
     4741 agcgttcctg cgtcaaaagc gccataaatc accaccaagt cgtctgtcac tgacaccaaa
     4801 tctttactga cacactcaac aagtgtctgg gaaagccgtt ccattgggga ggccgcagaa
     4861 agattgaggg cgacggtcaa gagtccggcc tcctgcggcg cttttgcctg ggcttgggtg
     4921 tcaacgacca cgatgagttt gtgatcttgc accagattgc ggactcgagt ccattcggct
     4981 ttgcccggcc agagaagcgc gatcgctgct gccttggtgg ctttggctac ctgaactcca
     5041 tgttctagga gaagtttgac gttctcggtg agtcgcagga cgtcggtagt gggagggggg
     5101 gaaacggcat gttttacgca gtcggccggc ggggaagttg tctcatcttt gatcatagac
     5161 agtcgaatta ttagcaagaa gaaatttagc aggacgacgg gcgatcgcac tcccaagctg
     5221 ggcttgatac tcgcgaagta aacggcactg ccgtgtcaag acatccctgg acacagcctt
     5281 accgggcacg cgacgatcaa ttaacaccgc aaaatcatca tacccctttc tgtccacgtg
     5341 gacaacggcg ttttacactg ggttgaagag ggcttatcga agtaggaacg acattggtcg
     5401 tagatcggta aggaggataa ggccggattt tgatcgatgt tgacacaagc ttgaggcgcg
     5461 ttagaatcca agtgaattgc ctgttttccc aacggaaaaa gggttgcgtc tcggggaagt
     5521 ggggggcgct gtacattgat ggcggttgat gcgaccattg ttcagcggtg tctgaaagga
     5581 cgtcgctgtc tttattaaaa gggtcatatt atgaaatgtc aaaaatgcga tcgccctgcg
     5641 acgtaccata ttaccgaatt gaccggtccc acgccggtcg aggtccatct gtgcgagcag
     5701 cacgcccagg agtacttggg tcggggtagt gagccatccg tcgagatgac gcaagtggcc
     5761 gagcagatgg ctcagcagtt ggccaagcac atggtggttg gtcaagccgc cgaggaaatg
     5821 aaacggcttg accagcaggc atgcccctac tgtggaatca ctttttataa attccggaat
     5881 cagggacggt tggggtgtcc gcacgattan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnna cgagtagcac aatgttaact gtcgatgatt tgatgcaggg ttgtggggaa
     6061 tggctccagg gtaagggtcg ggataacgac atcgttattt ccacccgaat acgcctggcc
     6121 cgtaatctgg cggactttcc tttcaccagt atcgccagtg aagcggagca acggcagatc
     6181 ctggatcgtg tgagagatgt cattgcggcc agtccattct ctgagcaact tctctatgtt
     6241 cccatggagg agctttccga gctagaccgc cagtttcttg tggaacgcca tctcatcagc
     6301 cgcgaattgg cagaagggca ggggcccaag gccgttttta tcgaccatgc cgagcgtttc
     6361 agtatcatgg tgaacgaaga ggatcatctt cgaattcaag cgatgcagag cggttttgaa
     6421 ccggagccaa cttgggccct gattaacgag ttggacgatt acctcggaca gaagctggtc
     6481 tttgcgtacc atcaagagtg ggggtttctc acggcctgcc cgacaaatgt gggaacgggg
     6541 atgcgggtaa gtgtcatgct gcatttgccc gcctcggtca tcgttaagca gattgaacag
     6601 attttccgca gcctagagaa actggagttg gtcgtgcggg gattgtacgg ggaaggctcg
     6661 caggctctag gcgatttcta ccaggttagc aaccaggtga cgctgggccg tagcgagggg
     6721 gagttgattc gcaagatgct ggatgtcatc ccggcgattg ttggcgccga aaggaaggcc
     6781 cgacagttcc tccttgacga aggaagccag cgggttcacg accgaatcag tcgagcatat
     6841 ggtgtgctaa agacggcgca gattatttcc tcgcaagaga ccctggaatg cctgtctctg
     6901 gtgcggatgg gcgtccacct tggtttggtc aaggatattt ccatcccgct tctcaatgag
     6961 ttgttcttgt acacccagcc agcccatctt caaaaggttg tcggtgtgga gatggacagc
     7021 gctgaccgca acgtagagcg ggcacggttc ctgaagaaga aactcggaac cgcggggcca
     7081 atggggtaaa ggaccgcatt ggcaggagtc tcacccttat cgatcacacc atccctgtat
     7141 tcacacctgg acttcggcgg caagatctct cgcccccgta taacgttccc gaatgggctg
     7201 gaccacgtct cggatgaatt cgtcaacctg ttctcgggaa cgccctacgt atttcagtgg
     7261 gtcggtcaca ctttcaaaat tgacctcagc aaaggctggg tcggccttaa gacgctcgat
     7321 gagatcgttt tcaccccctt cctctttgat ctttcgggca acctgatggc tatgctgccg
     7381 gattcgctcg tgcagttcct gccgatcacc gcccgcttgc acggcagcca tgaggatgtc
     7441 ttcagtcatc atgaaaggca attctgccaa aaggttcttt tcgataacct tgggataaac
     7501 aaccagaccc gagaagacgt tgcagcagag aatgaggatg gcatcgattg ccaaaaacgc
     7561 ttgcgggatg acgaggcggc gattggcact atcgtccagg gttcgttcca accactggac
     7621 gccatgcgtg agggtggtgt tcgcctccag actcatcaca tatcgcgcca ggccgcaaat
     7681 tcgctcactc cgcatggggt ttctcttgta tgccatggcg gaagagccaa tctgttcttt
     7741 ttcaaacggt tcttcgatct cttttcgatg tgccaatagg cgaatatcgg tagccacctt
     7801 gtgagcagtg gcggcaattt gggcgagggt cgcaagaatt tgggcatcga tcttccgaga
     7861 ataggtttgt cccgtgatga catagctacc aggaaatccc atcttctggc aaactaattg
     7921 attgagcttt ttcaccttct cgtgatcgcc gttgaacagc gtaagaaaac tggcctgcgt
     7981 tcccgtggtc ccctgaacac cgcgggcctg taactgactg agtcggtgtt ccacctcctg
     8041 caaatccatg gcaaaatcgt acgcccaaag acaagcgcgc cttcccaccg tggtgggctg
     8101 tgccggttgg aaatgggtaa agctgaggca gggcaagtcc cggtatttgg cggcaaacgt
     8161 tcccaaatga tcaatggctg ctgccagacg acgggcaatg agcttcaacc cttcgcgcat
     8221 gagaatgagg tcggtgttat cggtgacata acaactggtg gcacccaagt gaataattcc
     8281 gcgggcggaa gggcaaacat caccaaaggc atggacgtga gccatcacgt catgacgaaa
     8341 tcgccgctcg tattccgccg ctttttccca atcaatgttg ctgatctggc ttttcatctc
     8401 ggcaatttgc tgttcggtaa tcggaagccc cagctcggcc tgggattcgg ccaagatgac
     8461 ccacaaccgt cgccaggttt cgatgcgccg ccgaggcccg aaaagttcgc tcatttcctg
     8521 tgaggcatat cggcccacaa ggggattatc gtacatggca tatggatcac tttttgacat
     8581 agcggattac cttctgcttg gaggaaccga ttctagcgac atgcattacc gagtttcaca
     8641 tattggttac gtcatcaaca tggtcttcgc tcagtaggga atccatggcc tgtgcaatct
     8701 cggatgcgtg ctctgtcgag agtgcataga cacggcagat gcggtaactg agcggaatct
     8761 ggcgatagag ttccctggca tcgaggctgc gaaccgcatt ttgggccggg tcgtacaaga
     8821 aattctgccc ggcggcgggg gcacgtgttc ccggacgatg aacatgccgc ggtgggtcaa
     8881 agcggagcgg catatctcgc aaatcggaag gaagtcggcg acgaactgct tcctcaaata
     8941 ggtgctcatc gctgaaaacg ctcatttctt ctgctttgcc cggcccaaaa aagatggttc
     9001 gttcacagac cattttccag cggattttgc gggctaacca ttcccgccac tggtgcccca
     9061 aacgcgcccg ccgctcgtcg gacgattcgg cccagcgctc gacatcgacc aacagtgacc
     9121 agtccgtcaa acgcctatat tcttctaggt gatctaaagg attgccggga aatagcaggt
     9181 tcgcactctc ctgaaacaag tcctgaagag acaggtcgat cgaccggacc gtcctgtgga
     9241 agtagatgga ccgaaacagt tccgcccgca ctgtgatgaa gcgcaccagt gccgacaatc
     9301 ctcgttcgtg gatcgtcaca ccttgaggtg tgaaaaagct gtaatggatg aggcgttcga
     9361 ggtcaaatgc cttctgactg tatcccgaca tgtaggcatc tcgtaggacg aaatccatgt
     9421 tatcgaccgt gtagatgccg ctaaacaggc ctcgcagcaa gcgtagccaa agtggcgccg
     9481 ttcgatcgga tccgcttctc ggacgcatca taagaaagca gacctgctcc gggtccagtt
     9541 cttcgtggga ctcgagtttt ccaaatggat ttcgccgaat gccacggatc aggggcgcca
     9601 ggtcgcggcg aatgatctca gctccaagtg actcgtgatt gagctggtaa cgggatagat
     9661 aaaactgatc gaaaaaatgg ccgaacggtc catgtcccac gtcatggaga agtccggcta
     9721 ctcgtatcaa tgagaccacg taaccgcgac ttgggacatt ggggtcactt gcagccagcg
     9781 ttgggtagag ttcgttggcg atccggccgg caaggtgcat cacccccaac acatgttgaa
     9841 atctggtgtg cgtcgcggag gggaaaaccc accaggcggt ttgaagctga tggatttggc
     9901 ggagacgttg aatccaagga tggtcgataa gctcctgctc cgagacctcg ccagtggata
     9961 cccccctcct cgagacgaat ggtatgtacc catgaatggg gtcatggcta aggctctctt
    10021 ggtcgtaatc ggggcgcatg gacacaacct cccggacggg tgagcggtcc tctttcaaat
    10081 aacgggggtt tataatggta cggctgtgca tccgtaaacg gctattttcc gggtgaacat
    10141 gtcccaggaa aaggggcaaa gggccaagta tcacgaaggt tcaatgtttt ttgagcgatt
    10201 tcaatagcgt tgttcgatga tcggttagaa aagaagaagc tgcatgtatc gagcgatcgg
    10261 acttggggtt ttgctaatgc tgttgccgac gttgtcagtt tctgcccaac caccgcagcg
    10321 gacgccccca tcgggcggac cgggtgccag ggggaccacg gcttttgaag tcaagacctt
    10381 gcccaaagat gatatcgaga agcagatctt ggatgtcatc gccgaggtag gagcgaatca
    10441 gcgagcgggc aatatgatcg tgccggagga agatggtcgg cttctccgag cttgggcaga
    10501 agccgtgggt gccaagcatg tcgtggaaat cggaacatcc gtgggttact ccggcctttg
    10561 gttttgcctg gtcctgaaga agaccggtgg taaacttaca acgttcgaaa ttgacgagag
    10621 acgtgctgct caggccaaga aaaactttga aagggcaggg gtcagtgaca ttgtcacgat
    10681 cgtccttggc gatgcccacc aaactgtgac ccagcttaag gagccgattg acgtactttt
    10741 cctcgatgct gacaagaccg gctacatcga ttactttgag aaactttctc cccttgtccg
    10801 gcccggtggt gtggtgattg cccacaacat gacaccacgt tcggccgatc ctcgatttgt
    10861 caaggccatt acgacgacac cggagtatga gacggtcttt ctccatatgg agggggcagg
    10921 agtctcggtt tccgtcaaga agcactaaga cctggtttgg cgagcaaacg cggagttaag
    10981 agtctatccc aaaaagttcc ccctctccct ccgggagagg gttggggtga gggcaagcga
    11041 tcgtcggagt cgatcgagac aaccggcagc gcttcgagcc acccccaccc ctgcttttct
    11101 cccgccaaac gggcgggggt gagattacgg gataggctct aagccacgag ggccagaata
    11161 cgatgggata gggaacggcc taagtggctg gccggcgagg cgaagcgctc cttcaaatgg
    11221 cctcgccgcc gcgttctccg gtgcgaatgc ggatgcaatc ttctaagggt aggacaaaga
    11281 tcttgccgtc gcccacttga ccttgcggac ctgtcctcgc gcccttgata atggcgtcga
    11341 tggtcggttc gaggaaattg tcgttgacgg cgatcatcag ttggaccttt cgcgtcaagt
    11401 tcacgctaaa ctcatgaccg cgaaaggcct ccgcgtggcc tcgctctcgc ccgaatcctt
    11461 gtacgtccat gaccgtcaag cgaaaaacct ccacatcggc caaagcctgc ttcactgcct
    11521 ccaagcgatg tggctgaata atggcgatga tcagtttcat gggattctcc gcgcggttat
    11581 cactcttggg gctatcctag cacggtcgga tgcggtgggc aagtcggctc tggacgaaga
    11641 atcccggtcg tacccacaca ggcttcggta acccttcgtc agcggatgac tttgtgggca
    11701 cgggatgctt tagcgagctt caacacttag
//
LOCUS       DAOSUT010000129        11607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3151_length_11607_cov_8.901143, whole genome shotgun sequence.
ACCESSION   DAOSUT010000129 DAOSUT010000000
VERSION     DAOSUT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_11607_cov_8.901143"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1318
                     /gene="dprA"
                     /locus_tag="PLD05_11625"
     CDS             200..1318
                     /gene="dprA"
                     /locus_tag="PLD05_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOP78133.1"
                     /translation="MSDDAYSARLLQHLRLSLIDGVGPRLYRLLLGRFGTPDDVFAAS
                     EDELLAIPGLGRRLAERLRHPPSLEEVRHLVEECSAAGVDILWAEHIRYPRMLKEIPD
                     PPAVLFTRGELRPEDELAVAIVGTRHASTYGLKMAETLAAGLARAGVTIISGLARGID
                     AAAHRGALAAGGRTIAVLGSGVERIYPPEHGALAEEIMEHGALISEAPPNAPPHGGMF
                     PQRNRIISGMSVGVVIVEAGENSGALITARHAVEQDREVFAVPGRADSPNARGCNRLL
                     RDGAKWVESPQDVLDGLGPLVRPVERPDGTVVRRPLELKLSPLEQQVLQTIRDDPTAI
                     DEVIAVTGLAASQVLATLSVLEMRGVIRRVGGTNVVRR"
     gene            complement(1528..3144)
                     /locus_tag="PLD05_11630"
     CDS             complement(1528..3144)
                     /locus_tag="PLD05_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOP78134.1"
                     /translation="MTVFSRRQQVLWFLALISLLVPYLGLMIIGGYALWQNGWFWIYG
                     SVTAGVTALVCGLAFYLHKRRDLQALEPLKFECPQDWPPREQAAWSKVEHLAQQATRG
                     EIPVESIDDFRALLVRLVREIAQHYHPEHDDAYLDTPLPQLLKIVEKVCQELRQVVQN
                     HVPGAHILTLRDWRTIKEAYDFFEPLYRLIYWAYRIGSIPVSPVSALWREIRGFVAGQ
                     ALNLSVKELKAELVSYVIHRMGFYLIELYSGRIILDDKEFAEVRRQAEQRELQTAEPE
                     VPDTPPEFIRIVIVGQLKAGKSSLINALRGTWQAQTDVLPCTDQVQCYLMRPLAEGDV
                     NDPRPVLPGLLVLVDTPGYDSLGDGRNFAPSLDEVKKADMILLVCSARTAARKADHEF
                     LQAVRQWFQAHPHWIMPPLIVVGSFIDELRPRHEWNPPYRWRDVKEGESLSPKEQALQ
                     LFLEALRRDLELASGDPVIPVCTHPERTWNIAEGLIPTVAAYWEKARAVRDNRHREAY
                     QKTPYRLLWRQVKNATGSTIRLALKKIGIR"
     gene            complement(3156..4394)
                     /locus_tag="PLD05_11635"
     CDS             complement(3156..4394)
                     /locus_tag="PLD05_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOP78135.1"
                     /translation="MGTRIFGWMTQYWRALFHPQVDEEAVQQSLRAIKERLPTPVVWL
                     LGKAQSGKTSIIHALTGDPRAKIGNGFEPCTKSSLRYAFPDKEFPLVEFLDTRGLDDA
                     SYDPSEDVKWHEQQSHLVMVVMRVMDHAQGSLLAIVEDLRRRKPDWPIIVVQTCLHEG
                     YTWREPRHILPYPFDKEPLPSEVPPDLTRSLLAQRAWFRGETIRYVAIDFTLPEDGFE
                     PQFYGLQQLWRAIEEALPLGLWSFLKEGGPLSQSLAEPYAQAAEQTILAHTVLAAGAA
                     AVPVAAIDIPLIVAIQFHMAQRIAKIYGQSLTGDRWLDLMGTLGWSFLTRLGVRFISR
                     ELLKLLPWVGVPVAATFMGASTYALGRMLAWYFAEIKHGHLPDQHALRRMYEKEWERA
                     RDRLGGVFSSSSKEGPEKDR"
     gene            complement(4394..6973)
                     /locus_tag="PLD05_11640"
     CDS             complement(4394..6973)
                     /locus_tag="PLD05_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP78136.1"
                     /translation="MRLPLLPERSVESALILMWLVVVGLCPWNVGCRRPQISPEAKSW
                     PAVAEDPREKEGEPGTWPMFRGPNAQGIGVEAHPPVAFGEDHNLRWRVAVPGSGNSSP
                     VVWHDRIFLTTAEGGRSDANGVVLAYRRSDGALLWRYEVGPVKGRTHTKNGYASPSVA
                     CDGERVVAFFGSAGLVCLDLDGNLLWRKDLGPQEHEYGVGSSPIIVGDAVIQLCDREQ
                     DSFLIALDKKTGDELWRTPRPTVGCWTTPVVFSQGNNKGQPVLVVNGGKLESVGRVAA
                     YDLATGRELWSRDEIETLVTPVAVFAENILISMSGRNGPILALSFPQEGAAQAPAMLW
                     KLPRGGPYIPSGLLYRNRLYILSDARYVACYNPGNGALIWRGSLNGTFTASLVAAEGR
                     IYAVNERGIVFVLNAGDEFRLIAENRIGGRCLATPALVEDDFVLRTDKFLYCFSEKAN
                     RETKPEDSPAVKQTQSGDAPPAQPQEPEQLKTEPSNSNAMVPAEIPADGWPVFRRTPE
                     ATGFVISKIGANLKTRWIFSTEKDQFTATPIVAENTVFLGGAEGIFYAVDLKEGTVRW
                     KAEGYPGFAAPAGYYKGKVYVGDLDGRFHCFQAKDGQHLWQLETGAEIDGGPAFVTLP
                     SGKTVVLVGSQDSNLYCVDADTGEIVWKAASGDQIRCTPPVIKGRTFVAGCDGKLHVL
                     ALEDGRELAAVDLGGPTGSTPALVDSWAYVGTENGVFYGIDWEKAQVVWKFEDTKSGD
                     AFRASAAVNERYVVVGSRDRRVHCFDRITGEHRWQFATRRYVDASPVIVRHKADQNPD
                     EDWVIAASLDGHLYGLRIGNGEKVFDITVGGGMSASPVVAERCLIVATEDGDLACFVS
                     AGE"
     gene            7099..9591
                     /locus_tag="PLD05_11645"
     CDS             7099..9591
                     /locus_tag="PLD05_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOP78137.1"
                     /translation="MSRNAVASFTSMAAWHQPMVPVVLAFGLGIALDRWVWGDTSPPA
                     LWIGYMMMGLAVLAISRWMSTRSQNRWALAMLFLSVLWAGAARHHLHWRVFPDNHIGR
                     FASDEPQPICLEAVLSSVLEPSALSAEAMTFPEPKPFLRGVAKAVALRDGTTWRPVSG
                     RVLLYVAGSESPYQPGDRLRIVGRFQRLAQPQNPGQFNLREYRRADRILAQVTVDYPE
                     GITHIDSATSWQPLAILGRWRAAARLRLHLLLPESDHALAQALLLGFRSEIPDDFEEA
                     LLKTGTIHLLAISGLHVGILAGGFSLGLQLVRFPRRLRPVALLLVIVVYVALAGGQPS
                     TVRASITLGLFLVGHLLYRSTFSINTLAVAAMTLLILNPSDLFRVGPQLSFLAASVLL
                     VVQSTKLQPTDPLLVSGFGPPSAWARFIRSLRQRVVSLLLLSVTVWLITLPLVIQQFH
                     ILNPLSVILTPILVFPITVALLTGFLLILLGPVLSPLTACLAWLCHVSLSITESVIVS
                     TCQNLPLYWWLPSPPGAWVMGFYVLWLVFLIWGYRGPRWLARPLQVGLSVWLLCGALF
                     LAWPRKASPHEFRFTLLSVGHGLASVLELPNGEVWVYDVGSFGNHRRPAQILAGYLWS
                     RGYSRIDKVVLSHADLDHFNGLPLLVDYFSVKKIVVSPQMFCRSEPALEELRRLITVS
                     GIRCETVASGQRWDSDGCRLEVLHPPPDFSTPDDNAHSLLIVATYAGRRLLLPGDLEG
                     QGIRAILDRPSVKVDALVSPHHGSVRGQAGDLVRWCRPRWLLVSGGQSSLESVKEHFQ
                     GQVKEILHTAETGAITLVFSSRGCEVILWNTL"
     gene            complement(9825..10022)
                     /locus_tag="PLD05_11650"
     CDS             complement(9825..10022)
                     /locus_tag="PLD05_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78138.1"
                     /translation="MKKSLALLVGVMLVWGGLFIGCSKSQQNAAEDPARPEAIEDLNQ
                     VLPAPDASGRIGGGQPANTNP"
     gene            complement(10136..11185)
                     /locus_tag="PLD05_11655"
     CDS             complement(10136..11185)
                     /locus_tag="PLD05_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78139.1"
                     /translation="MRRLRFRKRGFTLVELLVVIAIIGILIALLLPAVQAAREAARRS
                     QCTNNLKQLGLALQNYHDVTKSFPPSGILCGDLRVPPVPSTPSAVPYHHTWLVMILPY
                     VEQKALYDQIDKSRPIYNTPLLGSTELAMSKQVQAFLCPSNPVLDLGKTRNMAYTNYA
                     ASEGYHWWTTATVDTNWGYWAVFQGTKSPVDLSGMFTILQTRTMSDLLDGTSNTIAIA
                     EVNSTGYKNGPITTSGTGVPRLDTNERVFRAAFVFTGTNGECCETGRWRNPDGSGPST
                     AARWFPGASPHAFSPTFLAAWGPNANWHGASSLHPGGLNAGFADGSVRFFAQTIEWHI
                     WAKLNAISDGHSVQQ"
BASE COUNT         2366 a         3616 c         3178 g         2447 t
ORIGIN      
        1 cccgacccct ctcccgccga acgggcgagg ggagattatg ggataggctc taagcacgag
       61 ccaccggcaa acagtagccg tcgggaaccc tcccatagtt gggcccaacg ggctaaccgc
      121 ctaaaatagc cagacacgac ttgtgacggc cagcttgaac ttgaagcatt catatccgcc
      181 aactgtttaa tccagattca tgtccgacga tgcgtattct gcccggcttc tgcagcatct
      241 ccggctgagc ctgattgacg gcgtcgggcc gcgattgtac cgcctgctct tggggcgatt
      301 cggtacaccc gacgatgtct tcgctgcctc ggaagatgag ttactggcca taccggggtt
      361 ggggcgacgc ctcgcggaac gcctccgcca tcccccgtcg ctggaggaag tgaggcacct
      421 tgttgaggaa tgctcggccg cgggggttga tatcctctgg gccgagcaca ttcgttatcc
      481 gcggatgctc aaagagattc ccgatccgcc cgccgtcctc ttcacccgtg gggaacttcg
      541 cccagaagac gagctagccg tggccatcgt gggaacacgc catgcctcca cctacggatt
      601 gaagatggcc gagacccttg ccgccgggtt ggcccgcgct ggggtgacga tcatcagcgg
      661 cctcgcccgc gggattgacg ctgccgccca ccgcggggcc ttggcagccg gtgggcgaac
      721 catcgccgtc ttggggagcg gtgtcgagcg catttatccg ccggagcatg gggccttggc
      781 cgaggagatc atggaacacg gggccttaat cagcgaggcc ccgcccaatg ccccgcccca
      841 cggcggtatg ttcccgcaac gcaatcgcat cattagcgga atgtcggtgg gcgtcgtcat
      901 cgtagaggcc ggcgaaaaca gcggggccct gatcacggca cgccatgccg ttgagcaaga
      961 ccgcgaggtc tttgccgttc ctggtcgggc cgactcgccc aatgcccgcg gatgcaaccg
     1021 cctcctccgc gacggggcca aatgggtcga atcaccgcag gacgtcctcg acggcctagg
     1081 cccgctcgtc cggcccgtgg aacgtcccga cggcaccgtg gttcgacgac ccctcgaatt
     1141 gaaactcagt ccgttggaac agcaggtcct gcaaacaatc cgcgacgacc caacggccat
     1201 cgacgaggtg attgccgtca cagggctagc ggcctcgcaa gtgctggcaa ccctcagcgt
     1261 gttggagatg cgtggtgtga tccggcgcgt tggggggacc aacgtcgtcc gccgctgaat
     1321 cgacaggatg ggcacggcaa caaagggcgg ccgtgggcgc tctgcgcttc tggggcaggc
     1381 acgggggcct gcccctacac gtttcaccgg gagggtcatc gtcctcgatg accaaatcgc
     1441 tgcaccgcgc aaacgtccgc cgcgtccaca cgcacgagga cgccgaccgt ccctcccccg
     1501 gctctcaaag ggaaaaacac cccatcgtca gcggatcccg atctttttca gggccaatcg
     1561 tatggtgctg cccgttgcat ttttcacctg ccgccaaagc aagcgatatg gtgtcttttg
     1621 ataggcctct cgatggcggt tgtcgcggac ggcccgcgcc ttctcccagt acgcggcgac
     1681 ggttgggatc aagccctccg cgatgttcca ggtcctctcg ggatgcgtgc acacagggat
     1741 caccggatcg cccgacgcta gctccagatc gcggcgaagg gcttccagga aaagctgaag
     1801 ggcttgttcc tttggcgaaa ggctttcccc ctcttttaca tctctccagc ggtagggggg
     1861 attccactcg tgccgaggcc gaagctcgtc gataaacgac ccgaccacaa tcaggggcgg
     1921 catgatccaa tggggatggg cttgaaacca ttgccgaacc gcctgcaaga actcgtggtc
     1981 tgctttgcgg gctgccgtcc gcgccgagca caccaagagg atcatgtcgg cctttttgac
     2041 ttcatccaag gacggcgcaa aattccgacc atcgcccagg ctatcgtaac ccggtgtgtc
     2101 caccagtacg agcagacccg gcaggaccgg tcgaggatcg ttgacatccc cttctgcgag
     2161 cggtctcatc aaatagcact ggacctgatc ggtacaagga aggacatcgg tttgggcctg
     2221 ccatgttccg cggagggcat tgattaggct ggatttgcct gccttcaatt ggcccacaat
     2281 cacgatccga ataaattccg gcggggtatc gggcacttct ggttcggcgg tctgcagctc
     2341 tctttgctcg gcctgccttc gcacctcggc gaattctttg tcgtcgagaa taattcgccc
     2401 ggagtatagc tcaatcaagt aaaagcccat cctgtggatc acgtaagaga ccaattccgc
     2461 ttttagctct ttgaccgaga ggttaagggc ctgtcccgcc acaaagccgc ggatttctcg
     2521 ccacaacgcg ctaactggcg aaacggggat gctgccgatg cggtacgccc aatagatcag
     2581 ccgataaagc ggctcgaaga aatcgtacgc ctccttgatc gtccgccagt cgcggagagt
     2641 gaggatatgc gcgccgggca cgtgattctg cacgacctgg cgcagttcct gacaaacctt
     2701 ctcgacgatc ttgaggagct gcggcagggg agtgtcgaga taggcgtcgt cgtgctccgg
     2761 atggtaatgc tgggcaatct cgcggacaag tctgaccagc aatgcccgaa agtcatcgat
     2821 cgactccacg gggatttcac cccgggtagc ttgttgggca agatgttcaa ccttgctcca
     2881 agccgcttgc tcccgcgggg gccaatcttg gggacattcg aatttgagcg gttctagtgc
     2941 ctggaggtcc cgccttttat gcaggtagaa tgctagacca cagacaagtg cggtaacgcc
     3001 tgcggtaacg cttccgtaga tccagaacca cccgttttgc cagagggcgt atccgccgat
     3061 aatcatcagc ccaaggtagg ggacgagcaa actgatgaga gcgaggaacc agaggacttg
     3121 ttgccggcgt gagaacacgg tcattcctcc cttgatcaac ggtccttttc tgggccttcc
     3181 ttggaggacg aagaaaaaac accgcccagg cggtcgcgcg ctcgctccca ttccttttca
     3241 tacattctgc gtagggcatg ctggtccggc aaatggccgt gtttgatttc ggcaaaatac
     3301 caggccagca tccgccccag ggcataggtc gatgcaccca taaacgtggc cgccacgggc
     3361 acacccaccc agggtaggag tttcaacagc tcccgcgaaa tgaatcgcac tcccagccgc
     3421 gtgaggaatg accagcccaa tgtccccatc agatcaagcc agcgatcacc agtgagcgac
     3481 tgcccgtaaa tcttggcgat ccgctgggcc atgtggaact gaattgccac aatcaagggg
     3541 atatcaatag cggcaacagg gacggccgcg gccccggctg ccaataccgt atgggccaag
     3601 atggtttgct cggccgcctg ggcatagggc tctgctaacg actgcgacag gggaccaccc
     3661 tccttcagga agctccataa gccgaggggc agtgcctcct caatcgcccg ccacagttgt
     3721 tgcagtccgt aaaactgcgg ctcaaaaccg tcctcgggca aagtgaagtc gatggccacg
     3781 tagcggatcg tctcaccccg aaaccacgcc cgctgggcca ggagagagcg cgtcaaatcg
     3841 gggggcacct cggagggcag cggttccttg tcaaacggat agggcaaaat atgccggggc
     3901 tcccgccagg tgtaaccttc gtgcaagcac gtctggacga cgatgatcgg ccagtcgggt
     3961 tttcggcggc gaaggtcttc cactatggcg agtaacgaac cctgggcatg atccatcacc
     4021 cgcatgacca ccatcacaag atgcgactgc tgctcgtgcc atttcacgtc ctcgctcgga
     4081 tcgtaagacg catcatccag accccgagta tcgaggaatt caaccagggg aaactccttg
     4141 tcgggaaagg cgtaacggag agaacttttg gtgcacggtt cgaagccatt tccaatcttc
     4201 gcccgcggat cgccggtcaa ggcatggatg atcgacgtct ttcctgactg ggccttaccc
     4261 aaaagccaaa cgacgggcgt cggcaaccgc tctttgatcg cccggagact ttgttggacc
     4321 gcctcctcat ccacctgggg atgaaataag gcccgccaat attgcgtcat ccagccaaag
     4381 attcgggtcc ccattattcg cccgccgaca caaaacaggc caggtcgcca tcttctgtgg
     4441 caacaatgag acaacgctcg gccacaaccg gcgaggccga catgcctccg cccacggtga
     4501 tgtcgaacac cttttcccca ttcccgatcc gcaatccgta gaggtgccca tccagtgagg
     4561 ccgcaatcac ccaatcctcg tccggattct ggtcagcttt gtgcctgaca atcacaggcg
     4621 aggcatcgac ataccggcgg gtggcgaatt gccagcggtg ctcgcccgtt attcggtcaa
     4681 agcagtgaac acggcggtcc cgactgccca ccacaacgta acgttcgtta acagccgccg
     4741 acgcccgaaa ggcatcgccc gatttggtat cctcaaattt ccacaccacc tgggcctttt
     4801 cccaatcaat cccgtaaaag acgccgttct ccgttcctac gtaggcccag gaatccacga
     4861 gggcgggtgt ggaaccggtc gggcctccta gatcgacggc ggccagctcg cggccatctt
     4921 ccaaggccag gacgtgcaat ttaccatcac accccgcgac gaacgtccgt cccttgatca
     4981 ccggcggcgt acagcggatc tgatccccac ttgccgcctt ccagacgatt tccccagtat
     5041 cggcatccac gcaataaaga ttcgaatctt gcgagccgac gagaaccacc gtctttcccg
     5101 atggcagcgt cacgaaggcc ggtccgccgt caatctccgc cccggtctca agttgccaaa
     5161 ggtgctgacc atccttggct tggaagcaat ggaaccgccc atcgagatcg cccacataga
     5221 ccttcccctt gtagtatcct gccggagcgg caaagcctgg atagccctcg gccttccacc
     5281 gcacagtccc ctcctttaag tccacggcgt aaaagattcc ttcggcaccg cccaagaaaa
     5341 ccgtgttctc cgcgacaatt ggcgtggccg tgaactgatc tttttctgta gaaaaaatcc
     5401 atcgcgtttt caaattggca ccgatctttg agatgacaaa cccggtcgcc tccggtgtgc
     5461 gacgaaagac cggccagccg tcggcaggta tttcggcagg caccatcgcg ttgctgttac
     5521 tgggttcggt tttgagttgt tcgggctcct gcggctgcgc gggtggagca tccccggatt
     5581 gggtttgttt aacggccgga gaatcctccg gcttcgtttc cctatttgct ttttcgctaa
     5641 aacaatagag aaatttgtct gttcgcagga caaagtcatc ctcgaccagg gccggtgtcg
     5701 ccaggcagcg tccaccaatt cgattctcgg ctatcaatcg gaactcatca ccggcgttga
     5761 ggacgaacac aatcccccgc tcattcacgg cgtagatcct cccctcagcg gccactaacg
     5821 aggccgtgaa tgtcccgtta agacttcccc gccaaataag cgccccattg ccggggttgt
     5881 aacaggccac atatcgggca tcggataaaa tgtaaagacg attgcggtag agaagccccg
     5941 acgggatata cggcccaccg cgaggcagtt tccaaagcat tgccggagcc tgggcggccc
     6001 cctcctgcgg gaaggacaag gctaggatcg gaccgttacg accgctcatg ctgatgagga
     6061 tattctcggc aaagacggct accggcgtga cgagcgtctc gatctcatcg cgggaccaca
     6121 attcccgacc cgtcgccaga tcatacgccg ccacacggcc caccgactcc agcttgccgc
     6181 cattaaccac cagtacgggt tgccctttgt tgttgccctg ggaaaacacg accggcgtcg
     6241 tccagcaacc aaccgtcggc cgcggagttc gccataactc gtccccagtt ttcttatcga
     6301 gggcaatcag aaacgagtcc tgttcccggt cgcacagttg gatcaccgca tcgcccacaa
     6361 ttatcgggct ggaaccgacg ccgtattcat gctcctgcgg tcctaggtct tttcgccaaa
     6421 ggagattgcc atcgagatca agacagacca atcccgccga gccgaaaaag gccaccacgc
     6481 gctcaccatc gcatgccaca cttggcgagg catagccgtt tttggtatgc gtccgacctt
     6541 tgacaggccc cacctcatat cgccacaaca gggccccatc cgaccgacgg taagcgagga
     6601 caacgccgtt agcatcgctt cggccaccct ccgccgttgt caaaaagatg cgatcatgcc
     6661 aaacgacagg tgagctattt ccgctgccgg gaacggccac tctccagcga agattgtggt
     6721 cctcaccaaa tgccaccggt ggatgcgctt cgacaccaat tccctgggca ttcggaccgc
     6781 gaaacatagg ccatgtgccc ggttccccct ccttttcgcg agggtcttct gccacagcag
     6841 gccacgactt ggcttccggt gagatttggg ggcgccgaca gccgacattc cacgggcaca
     6901 accccacgac caccaaccac atcaaaatca aggcagattc gacgcttctt tcgggcaaca
     6961 acggcaatct catcttgagg tcctgacgtc aaaataggct tattcccaaa ccggccggcg
     7021 ttatctcata atgtcatcat tctctttgct tttgggattc acaataggtg tcaactctca
     7081 tagagatcac tgccacacat gtcaaggaat gcggtggcat cgttcacgag catggcggca
     7141 tggcatcagc ccatggtccc ggtcgtcctg gcctttggct tgggaattgc cctggaccgt
     7201 tgggtttggg gggatacctc tccgcccgcc ttgtggatcg gctacatgat gatggggctg
     7261 gcggtgctgg ccatctctcg ctggatgagc actcgtagcc aaaaccgttg ggccttggcg
     7321 atgctctttt tatccgtgct ctgggcaggg gcggctcgac atcatctcca ttggcgggtc
     7381 ttccccgaca atcacattgg ccgctttgcc agcgatgaac cccaacccat ttgcctggag
     7441 gccgtcctct ccagtgtcct tgagccatcg gccctgtcgg cggaggcgat gacctttccg
     7501 gagccaaaac cctttctccg cggcgtggca aaggccgtgg cgctccgcga tgggacgacc
     7561 tggcgtccag tttccgggcg agtcctgctt tatgtggcgg ggtccgaatc gccgtaccaa
     7621 ccgggcgacc gcctgcggat cgtgggaaga ttccaacggc tggcccagcc acagaacccc
     7681 ggccaattca atctccgcga ataccgccgt gccgaccgca tccttgccca ggttaccgtg
     7741 gattatcccg agggtatcac ccacattgac tccgcgacct cctggcagcc gctggccatt
     7801 ttgggacggt ggcgggcagc cgcccgatta agactccacc tgctgctgcc cgaatccgat
     7861 cacgccctgg cccaagccct cctcctcggc ttccggagcg aaattcccga cgactttgaa
     7921 gaggccctcc tgaaaacagg aacgatccat ctcttggcca tctcgggttt gcacgtcgga
     7981 attttggccg gtggttttag cctgggactg caactcgttc gatttccgcg acggctccgc
     8041 ccggtggccc tccttctggt catcgtggtt tatgtggccc tggccggggg acaaccctcc
     8101 accgttcggg cctccatcac ccttgggctt tttctggtgg gacacctgtt gtatcggtca
     8161 acgttttcca taaacaccct agccgtggcc gccatgacgc tcctgatcct caatccgagc
     8221 gacctcttcc gtgtgggtcc tcaactttcc tttctggcgg cctccgtcct attggttgtt
     8281 cagtccacaa agttacagcc cactgaccca cttttggtat cgggcttcgg accaccgtcg
     8341 gcatgggctc gcttcattcg atctttgcgc cagcgcgttg tgagccttct ccttctaagt
     8401 gtcaccgtct ggttgataac actacccctc gtcatccaac agtttcacat tctcaaccca
     8461 ctgagtgtga tcctcacacc gatcctcgtt ttcccaatca cggtggcgct tctgacgggc
     8521 tttctgctca ttttgctcgg accggtcctt agcccactga cggcttgtct cgcttggtta
     8581 tgtcatgtct cgctgagcat caccgaaagc gtgatcgtgt caacttgcca aaacctgccg
     8641 ctttattggt ggttaccctc gccgcctggg gcatgggtaa tgggttttta tgtcctgtgg
     8701 cttgtcttcc tgatttgggg ttacagggga ccgcgttggt tggcccgccc tctgcaagtc
     8761 ggcctgagtg tgtggctgct ttgcggtgcc cttttccttg cctggccgcg aaaggcctcg
     8821 ccgcacgaat tccgcttcac gctcctctcg gtggggcatg ggctggccag tgtcttggaa
     8881 ttgcccaatg gcgaggtgtg ggtttatgac gtgggtagct ttggtaatca tcgccgccct
     8941 gcccaaatct tggccggtta tctttggtcg cggggctaca gtcgtatcga taaggttgtc
     9001 ttgtcccacg ccgacctgga ccactttaac ggcctacccc tactcgtgga ttacttctcc
     9061 gtgaaaaaga ttgttgtgtc accgcagatg ttttgccgca gcgagccggc cctcgaggaa
     9121 ctgcgtcggc tgataacggt atcgggaatc cgttgtgaaa ctgtcgcatc cggccagcgt
     9181 tgggacagtg acggctgtcg cctggaagtc cttcatcccc cgccggattt ctccaccccc
     9241 gatgacaacg cccatagcct gctgattgtc gccacctatg ccggccgacg tctcctcctg
     9301 cccggtgacc tggaaggaca ggggatacgg gcaatccttg ataggccctc tgtgaaggtg
     9361 gacgccttgg tgtcccctca ccacgggagc gttcgagggc aggccggcga tcttgtccgt
     9421 tggtgccgtc cccggtggtt gcttgtcagc ggcggacagt ccagcctgga atcagttaag
     9481 gaacatttcc aaggtcaggt caaggaaatc cttcacacgg ccgagacggg ggccatcacg
     9541 ctcgtcttct cctcccgagg ctgcgaagtc atcctctgga atacgctata agcatccgaa
     9601 actcgcccag cgtgaaaagg ggcatccgcg gctggcaatg acaaccatgc gcctcgttgg
     9661 tgccgcaaat atttccacgg aatggataaa gaatcaacag ctccgaaact gttagggagg
     9721 gaacaccgga tcgatcaaga aagccaaagg cccgcaatgc aacctgcggg ctaacgtaaa
     9781 gctcgctgac cggttcgctc aacgccgttg aaatagcgaa cgctttaagg gttcgtgttc
     9841 gctggctgtc caccgccaat gcgtccgctt gcatccggtg cgggcagtac ctgattcaga
     9901 tcctcgattg cctcaggccg cgcggggtct tcggctgcgt tctgctgcga cttcgagcaa
     9961 cctatgaaca aaccgcccca taccagcatc acacccacaa gcagtgccaa agatttcttc
    10021 ataacctgtc acctcatcgg atttgtcatt gccttggcct cagttgtttg gaacacaccc
    10081 atgtgtcccc cacttataac ttggcacaag cggcaatttc gttgcctcaa accgctcatt
    10141 gctggacgga atgcccatcg ctgatggcat ttaacttcgc ccaaatatgc cactcaatgg
    10201 tctgagcgaa aaatcgcaca gaaccatcgg cgaaaccggc gttcagccct ccggggtgca
    10261 gactactcgc accatgccag ttggcatttg gcccccaggc agcaaggaat gtcggcgaaa
    10321 aagcgtgtgg cgacgctccg ggaaaccacc gtgccgccgt ggaaggcccg gagccatcgg
    10381 ggttgcgcca ccgtccggtt tcacagcact ctccattagt cccggtgaag acaaatgcgg
    10441 ctcggaagac tctctcgttc gtgtcgagcc gagggacacc ggtcccactg gtcgtaattg
    10501 gaccattttt atagcccgtg ctattgactt cggcaatggc gatagtatta gaagttccgt
    10561 caagcaaatc agacatggtt cttgtctgaa gaatggtaaa catcccgctc agatctaccg
    10621 ggctttttgt gccttggaat acagcccaat aaccccagtt tgtatcgact gtggcggtcg
    10681 tccaccagtg gtatccttca cttgccgcat agttggtata ggccatattc cgggtctttc
    10741 ccaaatcgag aacggggttg cttgggcaga gaaaggcctg aacctgcttg ctcatcgcaa
    10801 gctcggtcga acccaatagc ggcgtattgt agatcggccg agacttgtca atttggtcgt
    10861 agagtgcttt ctgttcaaca tagggtaaaa tcatcacaag ccaagtgtga tgataaggga
    10921 ctgctgaggg cgtgctcggc acagggggga cacgcaaatc accgcacaga ataccacttg
    10981 gcggaaaact ctttgtcaca tcgtgatagt tctgaagagc caggccaagc tgtttcaaat
    11041 tgttcgtaca ttgcgaacgc cgggcagctt ctcgcgcagc ttgcaccgcc ggcaggagca
    11101 atgcaatgag aatgccaatg atggcaatca ccaccaacag ttccaccaac gtgaaaccgc
    11161 gtttccgaaa cctcaaccgc ctcatgagac acctccttcc gggaaaagga ataaaccaaa
    11221 cgccacgcct atcggacaac ttgtttagcg ccaagccagt tctccctcct ttggcgcccc
    11281 gcattgtaat ttcgactagc caaccttgca agcattttgc gaaatgcatt tgagtttttt
    11341 gagcgagcca attttggcca ccggcaagtt gccccaaaac tcactcccga aagtcaatcc
    11401 ggtgttagca gcttggtcta tgcgccgttc gggccatcta acggcttggc gacggcgaaa
    11461 aaccacagtt cccgccgaat ggggtttccc gctatactga aatcttctgc gcctggtcgg
    11521 tgccaccgca cccggtctta aaataggtca tcgcagcaag agatcgaacc ttcgtgttgc
    11581 gccgtctccc ggcgtcgccc gccttag
//
LOCUS       DAOSUT010000130        11444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3200_length_11444_cov_8.856792, whole genome shotgun sequence.
ACCESSION   DAOSUT010000130 DAOSUT010000000
VERSION     DAOSUT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3200_length_11444_cov_8.856792"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PLD05_11660"
     CDS             complement(<1..1163)
                     /locus_tag="PLD05_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HOP78140.1"
                     /translation="MKAVSIRKWACLFAPVVAAALVGVWTSGHLRSAEPTRNVETVAP
                     VGATSYARVDTDEPFAAVVQRMQAQKEQILSRHRQLLEERYDLSNRPLPGAVMSRGKP
                     IQAGPRVKLPPGLTWEQLAEMSPEVIKQKGLWPKGFLPLPHPNHDEGGMLFPQHHIDE
                     IKRQEGRDLTRFDLDYDLPEHFLPEYPPAIYLTTRPDLGDVSRGQVVTLQNYFQLFNG
                     ILNPKQLEGLRLLVTPFPQQQFNATDDRRSLKPSQGVTCFDCHVNGHTNGTTHLLGDI
                     RPQAFRPRLDTPSLRGVNVQRLFGSKRALKTIEDFTEFEQRAAYFDGDPVIATKKGVN
                     VLERGSQVHFMAEFMAILDFPPAPKLDVFGRLDPTKATDQELRGEQVFFGKGR"
     gene            1542..3140
                     /locus_tag="PLD05_11665"
     CDS             1542..3140
                     /locus_tag="PLD05_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP78141.1"
                     /translation="MIRTNQAVHGTSVMRHSVTTWISRRVERVLRATALVAIAVVCSA
                     FSFCWAEQNPQGKERPNIVIILADDLGYGSVECYGAPADLVRTPNIDRLAREGQRFTD
                     ANTPSSVCTPTRYALLTGRYCWRTDLKHEVAGVFSPLLIEPGRINLASMLKKYGYETA
                     AIGKWHLGYGTGPRTDYTKELRPGPLEIGFDYHFGVPSNHGDITGVYVENHRVYGLRS
                     DKLDPTSVGTNFNNRPYLGLDAPQRVDEEVMPVLTTKAEQWIESRSGNKPFFLYFTPV
                     AVHNPVTPSEKTKGTSKAGPYGDWIHELDLSVGRILDVLDRKGFTPNTLVIFTSDNGG
                     VNKPTIPSESTDALKAGLPICGPFRGGKHDVWEGGFRVPFIVRWPGHVTPGTVCDEMM
                     SLTDVLATVAAIMGENLPPPDVGAEDSYNMLPAWLGQPYSSPIRPDMIVHSADGNFAI
                     RRGKWKWIEGTYHPDTRPGALRLRADQFHPQLYDLSTDIGETHDVAAEHPEVVAELSR
                     LLNHYRDCGHSRQVTPPDQVRREN"
     gene            3469..4584
                     /locus_tag="PLD05_11670"
     CDS             3469..4584
                     /locus_tag="PLD05_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOP78142.1"
                     /translation="MKSLCRRHWFELVLGVAWAVVFSLGISAWSLEAPRPQPPGAAAN
                     QPAPPRPPATANQPTPPRPPAPGAAPVPPAAPPKPPASSPAPPSPRERLPLPEEVSGT
                     ELLTKDGIQLRATYYPSNRGKDAAVVILLHGWKGSRKDFARIGPFLQQQGYAVFIPDL
                     RGHGESTQRLFPSPGTVTVDKMPPAEIARMVDMDLETCKRFLLQKNNAGELNIEKLCV
                     LGIDATATVALDWARHDWSWPIYPGLKQGQDVKALILVSPKWSVPGLNAQAALSHPAV
                     SGKLAIYIMVGKDSQKDLADAQRMYNIFARFHPKQDEQSPELQDLFFVKFPTSAQGVE
                     LLTRFEQAILQSIAFFIEQRVAKQPWPWQDRSRKKEG"
     gene            complement(4643..5311)
                     /locus_tag="PLD05_11675"
     CDS             complement(4643..5311)
                     /locus_tag="PLD05_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOP78143.1"
                     /translation="MDRGFIVFLIVGGLSLGTVWAILSRPRESRSFSHMESLPTRPGP
                     NLAELRSPSSFPEDRLLPSSQELSDAINLTQPPAPSSDLLLSLSPPPAPSVTPFFPKP
                     QTLLKIEQRNVPEKTSKTSGGRLPSGVPSQARNARPGQSFDRLEPAQQVVRHIVADGE
                     TLPIIAQRYYRDITLWTVIYQANQDRLKSPELLPIGTELVIPPIPQDLQSTPFGAEAT
                     RETP"
     gene            complement(5578..9003)
                     /gene="secD"
                     /locus_tag="PLD05_11680"
     CDS             complement(5578..9003)
                     /gene="secD"
                     /locus_tag="PLD05_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOP78144.1"
                     /translation="MDRRVTSLRRFGLTLGVPVVFAIFALVLAAPLPVGSSARAEQAP
                     AVAEGPQLPAASVTNQGENAQSSGTVAPETPAPSEKVAPSADQEQPEKQTAVATAQET
                     PAPGGQTSPAPQAAEKAPGEGQLSGLLNALIYLGFLLLIIGLPVGLAYFICEQWRLRE
                     YFGRLSFVFFTIVVAIVIILTRWPPKLGIDLSGGVIMVYEIEGAVRQPGERAQADRDQ
                     IDMDQLIAALNRRVNPSGVKEVTIRRFGPQQIEIIVPEVNEEEVARLERVISSIGTLE
                     FRILANQRDHRRLIERAMALSPDEQELYDDRGNLLAWWVPVAKGQEAAIGTGGEIAVR
                     MGRYRNEDWLQVLVVKDRYNVTGADLFRVSEDVDERMQPCVSFVLRAGSAQRFGALTS
                     ENRPDATTGFERRLGIILDGYLYSAPNLRQPIFDRGQITGIPTREEVQALIQVLQAGS
                     LPAVLSPTPISRLVTGPQLGRDTIGQSVTAMIVAAVIVFAFMVAYYRFAGIVACLAVV
                     LNLVMLVAIMILLKAAFTLPGLAGLTLTVGMAVDANVLIYERIREELSRKATLRMAIR
                     NGFEKAMSVIIDSNLTTMLTAVVLYFVGTDQVRGFAVTLFLGLVLNLYTAVFMARIIF
                     EIGERRKWIKQLYMMRIIGETNFDFTGVKKYALAGSVAVILLGLVALIARGPGVLDID
                     FTGGVAVEILFNKPKDIGEVRYRLRELRDLAVSDVRIEGEQPHRRYVINTSSPPGVEA
                     EQYLRSVKEKIAQVFGDELVHYSVQIENLSQEGTTYVPRRGFPNLMLAGSPWGSALAG
                     VLVGASGEETSQTGEAGQSGPATEQGPKLPEAQGANPTAQDTAAAETPSGPPADRFAG
                     GTRVLMKFDHKVNYETIESHLKEELAKLPEPDRNAAFEIYNKEYRRGDTTGYENWEIR
                     LALPPEKGLKVVEKVQKRFESTPYFPSSNTIGGKVASSTRVRAVYALILSVFGMIVYL
                     WVRFTKVSFGVAAAVALVHDVLVTLGLIALSAYLTGILGFLLIDEFKIGLSVLAAFLT
                     IIGYSVNDTIIIFDRIREVKGKAPYLTPRIVNTSINQTLSRTIITSLTTLFVVVVLYI
                     GGGAGIHAFSFTLLVGVITGTYSSIFIASPLLLWLSPQPVGPASQPQSLQHVS"
     gene            complement(9122..9490)
                     /gene="yajC"
                     /locus_tag="PLD05_11685"
     CDS             complement(9122..9490)
                     /gene="yajC"
                     /locus_tag="PLD05_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOP78145.1"
                     /translation="MTPGSLLFLAEAASGNTSSGVLMMLTLWIPLILVFYFLLIRPER
                     KERARRQAMLQALKKNDRVLTIGGIYGVVTNVHREANEVTLRVDDATNTRIRCTLSAI
                     AQVLGEETSAGNGKDAKSGD"
     gene            9872..10402
                     /locus_tag="PLD05_11690"
     CDS             9872..10402
                     /locus_tag="PLD05_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78146.1"
                     /translation="MNQFLLQQIRRRHILREAEGYLELGLFERALECLDRLPDRDRQS
                     FEARLIRAEILRAMGQYESALEIYESLIAQRPDDLNILLAMGWCHKRIGRIDLACADL
                     EKAKQISPRNSLVLYNLACYYSLAGRKQDALTHLEKALQLEPGLRKLIDTETDFDPLR
                     SDSDFIRLVYPDKGNR"
     gene            10526..>11444
                     /locus_tag="PLD05_11695"
     CDS             10526..>11444
                     /locus_tag="PLD05_11695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP78147.1"
                     /translation="MARSFRQAAVSRRFLAGTGGRVTRRNLIKGLGAAVTLPWIGKAL
                     AALSPKAEILEVKTISRLPHRYHGWPTVARRKNGQLVLVYSGGREAHVCPFGRVEMMT
                     SNNDGDWWTYPRVLIDSAIDDRDAGVLETAKGTLLVTTFTSLAYVPVLERAMADPGKG
                     WPSEKIDQWRAAHERLTEAHRKSQLGVWMIRSEDGGVTWSSPYDCLIDSPHGPIQLRD
                     GRLLYAGKDLWRTENVGVCVSEDDGRTWQWLAVLPTREGDNPKQYHELHAVETTSGRL
                     IVQIRNHNSANSGETLQSESEDGGKTWSVP"
BASE COUNT         2632 a         3228 c         3103 g         2481 t
ORIGIN      
        1 catcgccctt taccaaaaaa gacctgctct ccgcgaagct cttggtcggt ggccttggtc
       61 gggtccaatc gaccaaagac gtctaacttc ggtgccggcg gaaagtccaa aatcgccatg
      121 aactccgcca tgaaatggac ctggcttccc cgctcgagaa cgttgacgcc ttttttcgtc
      181 gcgatgaccg gatcaccgtc aaaataggcg gctcgctgct caaactcggt aaagtcttcg
      241 attgtcttca aagcccgttt agagccaaac agccgctgga cgttcacccc tcgcagcgac
      301 ggcgtatcga gccggggccg aaaggcctgc ggtcggatgt cgcccaatag gtgcgtcgtt
      361 ccattggtgt gaccgttgac gtggcaatcg aaacaggtta ccccttggct tggtttcagg
      421 cttcgtcgat cgtctgtggc gttaaactgc tgctgcggaa agggcgtgac caagagccga
      481 agcccttcca actgcttggg gttcaaaatc ccattgaaaa gttggaaata gttctgcaac
      541 gtaaccacct ggccgcgaga aacatcgccc aggtccggcc gagtcgtcaa ataaatcgct
      601 ggcggatatt cgggcagaaa gtgttccggc aaatcgtaat ccagatcaaa gcgagtcagg
      661 tctcgcccct cctgccgctt aatctcgtcg atgtgatgct gagggaagag cattccacct
      721 tcatcatgat tcggatgtgg caagggcaga aagccttttg gccaaaggcc tttttgcttg
      781 attacctcgg ggctcatttc ggccaactgc tcccaggtca gcccaggagg gagtttcacc
      841 cgtgggcccg cctgaatcgg tttaccgcgt gacataacgg ctcccggcaa cgggcgattg
      901 ctcaagtcat aacgttcctc cagcagttgt cggtgacgcg acagaatttg ctctttttgg
      961 gcttgcatcc gctgcaccac tgcggcaaac ggctcatcgg tatcgacccg cgcataactg
     1021 gttgcaccga ccggagccac ggtctccaca ttccttgttg gttccgccga gcgaagatgc
     1081 ccactggtcc acacgcctac caacgcagcc gcaacgacgg gcgcgaaaag acacgcccat
     1141 tttctaatag acacagcttt caccgtgagg tccctcctct tccctaacaa gagttcgtct
     1201 tttcccccaa ggcgtccgga ccgcacgtcg ttaggatcac ccatacgcgc gttcccggtt
     1261 tcccaaccgg tcaaattcta ggcggccaca cctcaaaaaa caagtggatt gtccacgaat
     1321 tgcccgacga agatagaaga ttggctttgg cactggcgtc tccgggcagc atggctgtgt
     1381 tcgcgataca acctcccaaa ttgatgccgc ccaccacgag tgcaagcgat tttaccggcg
     1441 atagtggcga ttgacagacg tagtggacgt gactacaaat gcgccagtgc gaaagaattg
     1501 ctagttcatc gcagtttagc tcagatatgg ggaggtttgt catgatccgc accaatcagg
     1561 cggtgcatgg cacgtctgtc atgcgacata gcgtgacaac gtggatttca agaagagtgg
     1621 agagggtttt gagagccaca gcgcttgtgg cgattgcagt cgtctgcagt gccttttctt
     1681 tctgttgggc agagcaaaac ccacaaggca aggaacgccc caacatcgtg attatcctag
     1741 ccgacgattt gggttacggc agtgtggaat gttacggagc ccctgctgat cttgtgcgaa
     1801 cgccgaacat cgatcgtctg gcacgagaag gtcagcgatt tacggatgcc aacacccctt
     1861 cttccgtttg tacaccaacc cgctatgcct tgcttactgg tcggtattgc tggcggacgg
     1921 atcttaagca cgaggtcgcc ggtgtgtttt cgccgcttct cattgagcca gggcgaatca
     1981 atctggcatc catgttgaag aaatatggat atgagaccgc cgcgatcggg aaatggcact
     2041 tgggttatgg gacaggcccc cgcaccgact acaccaaaga gttacgtcct ggaccgctgg
     2101 aaattggctt tgactatcat tttggcgtgc cttcgaatca tggagatatt acgggtgttt
     2161 acgtcgaaaa ccatcgcgtg tatgggcttc gatccgataa actcgacccg acgagtgtgg
     2221 gcacgaattt caacaatcgg ccgtatctcg gtctcgatgc ccctcagcga gtcgatgaag
     2281 aggtgatgcc ggttcttacg acgaaggccg agcaatggat cgagagccgc tcgggcaaca
     2341 agccgttctt tctctacttc acgccggtgg cggtccataa ccctgtgaca ccttcggaaa
     2401 agacgaaagg cacaagtaag gctggtccct atggtgattg gatccacgaa ctcgatctta
     2461 gtgtggggcg aatcctggat gtgttggatc gtaaaggctt cacaccaaac acgctcgtta
     2521 tcttcaccag cgacaacggc ggagtgaaca aacccaccat cccgagtgaa tccacggatg
     2581 cgctcaaggc gggactgccg atctgtgggc cgtttcgcgg cggaaagcac gatgtgtggg
     2641 aaggtggttt ccgggtacct tttatcgtcc gttggcctgg tcatgtaacg cccggtacgg
     2701 tgtgcgacga gatgatgagt ctcaccgatg ttttggccac ggtggcagcc attatgggtg
     2761 aaaacctacc tccgcccgac gtcggtgccg aggacagcta caacatgcta cccgcctggc
     2821 tcgggcagcc gtattcctcg cccatccgtc ctgacatgat cgttcacagt gccgatggta
     2881 acttcgccat ccgccgggga aagtggaaat ggattgaggg cacatatcat ccggacaccc
     2941 gtcctggcgc cttgcggtta cgggccgacc aatttcaccc acagctttat gatctctcca
     3001 cggacatcgg ggagacgcac gacgtggcag cggaacaccc cgaggtcgtt gccgagctat
     3061 cccgtctgct gaaccactat cgcgattgtg gccatagtcg gcaggtaacc ccaccggatc
     3121 aagtccgccg cgagaattag ggtaatcttg gcgaggtctg taagcttatt gcgacccggc
     3181 gatctgcgat atagattccc acattcgggc actgaccccg ctaatttcat agattcgggg
     3241 tgaacaccgt agaaccctct gtagttcgtt cggctacggg tcgggacaag tagacctaac
     3301 attgcacgtt gaccgcgtcc ggggtgtttg attagactgg caatagcccg aagaaaaggg
     3361 catttccgac tcgcgtagcg tcggttacga agctgtggta gccagtccgt taacgaagct
     3421 gtcataaaca gtcgtggccc tcactggggc aagatgggag tgcgagatat gaagtcgctg
     3481 tgtcgaaggc attggtttga gcttgttttg ggagtcgctt gggcagttgt gttttcgcta
     3541 gggatctcgg cttggtcatt ggaggcacct cgtcctcaac cgccgggagc cgccgccaat
     3601 caaccggcac ctcctcgacc gccggctaca gccaaccaac cgacaccgcc ccgaccaccg
     3661 gctccgggcg ctgctcccgt tcctcccgca gcacctccca aaccgccagc gtcctcccct
     3721 gccccgcctt cgccgcgaga acgcctcccg ctaccggagg aggtaagtgg aacggaattg
     3781 ctcacaaagg atgggattca acttcgagcg acgtactatc caagcaatcg gggtaaggat
     3841 gctgcggtcg tgattctact tcacgggtgg aagggaagtc gaaaagattt tgcccgcatt
     3901 gggccgttcc tacagcaaca agggtatgcg gtcttcattc cagatttgcg gggacacgga
     3961 gaaagcaccc agcggctctt tccttctccg gggacagtta cggttgacaa gatgcctcct
     4021 gctgagattg cccgtatggt cgatatggat ctggagacgt gcaagcgatt cttgttacaa
     4081 aagaacaacg caggcgaact aaatattgaa aagctgtgtg tcttgggcat cgatgcaaca
     4141 gccacggtgg cgcttgactg ggcacggcac gattggagct ggccgatcta ccccgggttg
     4201 aaacaggggc aagatgtcaa ggccctgatc ttggtatcgc cgaagtggtc tgtaccggga
     4261 ttgaacgccc aggcggcact gtcgcatccg gcggtcagcg gcaagctcgc gatttacatc
     4321 atggtgggta aagactcaca aaaggatttg gctgacgcac agcggatgta caatattttc
     4381 gcccgctttc atcccaagca ggacgagcaa tcccccgagc tgcaggatct gttttttgtc
     4441 aagtttccta catctgcaca aggagttgag ttgttgaccc gttttgagca agcgatcctt
     4501 caatcgatag cgttcttcat cgagcagcgg gtcgctaagc agccctggcc ctggcaggat
     4561 cgttcccgga agaaggaggg ttaacgttag caacggcaga gcgaacgagt tttgtcacct
     4621 gccggtagtg cgcagtccac ggctagggag tttcgcgggt tgcctcggct ccgaagggag
     4681 tcgactgtaa atcttgagga ataggcggaa tcactagttc cgtcccaatc ggaagcaatt
     4741 cgggagattt taaccggtct tgatttgctt gatagatcac cgtccataag gtgatgtctc
     4801 gataataacg ttgcgcgatt atcggcagtg tctcgccatc ggccacgata tggcgaacta
     4861 cctgttgggc cggttccaac ctatcaaatg attgacccgg gcgggcattc cgcgcttgag
     4921 agggcacccc cgagggcagg cggccaccgg acgttttgga agtcttttcc ggaacattgc
     4981 gctgctcaat cttcagaagc gtttgtggct tgggaaagaa cggtgtgacg gatggcgccg
     5041 gaggggggct tagggagagc aataggtccg agctgggagc aggaggttgt gttaaattga
     5101 tggcatccga caactcctgt gaggatggaa ggagccgatc ttcgggaaaa gacgacggcg
     5161 aacgaagttc tgccaagttt ggaccgggcc gcgtcggcaa actttccatg tgcgaaaaag
     5221 agcgactttc ccgtggtctg ctcaaaatgg cccagaccgt tcctaaagag agtccaccga
     5281 caatcaggaa gacgataaaa cctcggtcca cgccgctggt cctgctttgt tgttccccgc
     5341 gagcgcagac atccctgtcg tgaacataaa acccagtccg caatcctgca aaacgacccg
     5401 cgctttaccc taagttctta tcgggcaacc ggcatccgat ggcatacccc ttccccaagc
     5461 tccagattct ccatcacatt aggagcttcc atcaccaccg gctcgaaggg aaatccgcac
     5521 cgattggcgg tgagctcaag cgcgacaagg cgagatcttt cagtttgccc ctctcggtca
     5581 cgacacgtgt tgtaacgatt gcggctgact tgcggggcct acgggttgcg gcgataacca
     5641 caacaacagt ggtgaggcaa taaagatgga gctgtacgtt ccggtaatca cgccgacaag
     5701 tagggtgaaa ctaaaggcat ggatcccggc accaccgccg atatacaaca ccaccaccac
     5761 aaatagggtc gtcaacgatg tgataatcgt tcgactcaag gtctgattga tgctggtatt
     5821 gacaatccgc ggtgtcaaat atggcgcctt cccttttacc tcgcgaatgc ggtcaaagat
     5881 gataatcgtg tcgttcaccg aataaccgat gatcgttagg aaagctgcca gcacagatag
     5941 accaatctta aactcatcga tcagcaggaa acccagaatt cccgtgaggt aagcactgag
     6001 ggcgatcaaa ccaagcgtaa ccaacacgtc gtggaccaaa gcgacggccg ctgccacccc
     6061 gaaactgact ttggtaaacc gaacccagag atacacaatc ataccgaata cagaaagaat
     6121 caaggcatac acggcacgga cccgcgtgct gctagcaact ttcccaccaa tggtgtttga
     6181 agaggggaaa taaggcgtcg attcaaaccg cttttgcact ttctccacaa cttttagccc
     6241 cttttccgga ggcaaggcta gacgtatttc ccaattctcg tagcccgtcg tatcaccacg
     6301 ccggtactcc ttgttataga tttcaaaagc ggcattccgg tcaggctcag gaagcttggc
     6361 cagttcctct ttcaaatggc tttcaatcgt ctcgtagttt accttatgat caaacttcat
     6421 gagcactcga gtcccgccag caaagcgatc cgcgggcggc ccactcggcg tctccgcggc
     6481 agccgtgtcc tgtgcggttg gatttgcccc ctgtgcctcc ggcagtttgg gcccctgctc
     6541 ggtggcagga cccgactggc cagcctcccc ggtctgagag gtctcctcgc cgctggcccc
     6601 cacgagcaca ccggccagcg ctgaacccca cgggctaccg gcgagcatca agttcggaaa
     6661 accccttcgc ggcacgtagg tagtcccctc ttgagacaga ttttcaatct gaacggaata
     6721 atgcactaac tcgtcgccga aaacttgggc aattttttcc ttgacactcc ggaggtactg
     6781 ttcggcttcg actcctggcg gacttgatgt gttaatgacg tatcgccggt gcggctgctc
     6841 gccttcaatt cgcacgtcgc tgactgccaa gtcccgaagt tctcgcaagc gataacgaac
     6901 ctcgccaatg tctttcggtt tattgaacag aatctcaacg gcgactccgc ccgtaaaatc
     6961 gatatccagc acacctggtc cccgtgcgat tagggcaacg agtcccaata gaatcactgc
     7021 gacacttccc gccaaagcat atttcttcac gcccgtgaaa tcaaaattgg tctcgccaat
     7081 gatccgcatc atgtagagct gcttaatcca tttacggcgt tcaccaatct caaaaattat
     7141 ccgggccatg aacacagccg tataaaggtt gagcaccaac cccaaaaaca acgtgacggc
     7201 aaacccgcgg acctggtcgg tccccacgaa atagagcaca acggccgtga gcatcgtcgt
     7261 taagttggag tcgataataa cactcatggc cttttcaaac ccattgcgga tggccatccg
     7321 cagggtagcc tttcgcgata attcttcacg gattcgttca tagatgagca cattcgcatc
     7381 cacggccata ccgaccgtca acgttaaccc tgcaagcccg ggcagggtga aagccgcctt
     7441 tagaagaatc atgattgcga cgagcatcac caggttgaga acaaccgcca agcaggccac
     7501 gatcccggca aatcggtagt acgcaaccat gaaggcaaag acgatgacgg ccgccacgat
     7561 catggccgtg acactctgcc cgatcgtgtc gcggcccaac tggggaccgg tgacaaggcg
     7621 gctgataggc gtcgggctga ggaccgcagg caaactgccg gcctgcaaga cctgaattaa
     7681 agcttgaact tcttcgcggg tggggatgcc cgtaatttgg cctcggtcaa aaatcggctg
     7741 cctcaagttt ggtgcggaat agagataacc gtcgaggata atccccaaac gtcgctcaaa
     7801 tcccgtggtc gcatcggggc ggttctcgct cgttaacgcg ccgaaacgct gagcgcttcc
     7861 cgctcgcaaa acgaacgaca cacacggttg catcctttca tcgacgtctt ccgaaacccg
     7921 gaagagatcg gctccggtta cgttatagcg atctttgacc accaggacct ggagccaatc
     7981 ttcatttcga tatcgaccca tgcggacggc gatttctccg ccagtgccaa tagctgcctc
     8041 ctggcctttg gccacaggca cccaccaggc gagaagatta ccccggtcgt cgtacaactc
     8101 ttgctcatcc ggggaaagcg ccatcgccct ttcaatcaag cggcgatgat cccgttgatt
     8161 ggcaaggatg cggaattcca aggtcccgat gctgctgatc actcgctcca accgagcgac
     8221 ttcctcttca tttacttcgg gaacgatgat ctcaatctgc tgcggcccaa agcgacgaat
     8281 tgtcacttcc ttgacaccgc tcggattgac acgccgattg agggccgcaa tcaactgatc
     8341 catatcgatc tgatcacgat cggcctgggc ccgttcgccc ggttgtcgca cggcgccctc
     8401 gatttcataa accatgatca ctccaccgct gaggtcgatc cccaactttg gcggccagcg
     8461 tgtgagaatg atcacgatag caacgacaat tgtgaaaaag acaaaagaaa gtcggccgaa
     8521 gtactcgcga agtcgccatt gttcgcagat gaagtaagcc aggccgacgg gtagccctat
     8581 aattaaaagt aggaatccaa gatagatcag tgcgttcagg agtccggata attgcccttc
     8641 ccctggtgcc ttctccgctg cttgcggggc cggagacgtc tgcccacctg gtgctggtgt
     8701 ttcctgggca gtagccacag cagtctgttt ctccggctgc tcttggtctg ccgatggtgc
     8761 caccttctcg gatggagcag gggtctccgg ggctaccgtc ccagaagatt gggcattctc
     8821 accttgattc gtcaccgagg ccgccggtaa ttgtggcccc tcagccactg ccggggcttg
     8881 ctctgcccgg gcagacgaac ccactggaag cggggccgca agaacgaggg cgaagattgc
     8941 aaaaaccacc gggacaccga gggtcaaccc aaaccgccgt aaagacgtca cgcgtcggtc
     9001 catcgtctta actcgctcaa tgcacaaaaa tcatccgcgc ctggcaataa ttcccctatt
     9061 tcacaatgaa gagggacttg acgtatccca tcatccccca atgccgcggg aacgttctgg
     9121 cctagtcacc tgatttggcg tccttgccgt tacccgccga cgtttcctct cccaacactt
     9181 gagcaatcgc actcaaggtg caacgaatac gtgtattggt ggcatcatcc acccgtaatg
     9241 tcacctcatt cgcctcccgg tgaacattgg taacgactcc atagatgcca ccaatcgtga
     9301 gtacacggtc gtttttcttg agcgcctgca acatggcttg gcgtcgtgcg cgctctttcc
     9361 gttccggacg gatcagcaaa aaataaaaaa caaggatcag cgggatccac aaggtcagca
     9421 tcatcaggac accactagac gtgttgcccg atgccgcctc ggcaagaaaa agcagactac
     9481 ccggcgtcat tggcctctcc tgacttggtc agtaccccgc tggcgacatg ccaattgaac
     9541 aatgccaatc atcacacatc tgtcccactg atgggggttg catgccttta accgccaaat
     9601 aatgaatctt aaagcttgaa aggtaatgcc acaaggggaa tgcgggcaag ctggggagct
     9661 gcaaaggggt gaggaggtca acccagcatc aacacaccca tggggataga gcaattctgt
     9721 cctctcctga gcactgcccc accgtctccg tgggaaaggt gatccaccat cagcccccct
     9781 tctgggcaaa agcgaaggca taaaatgaaa gtaggggtgt tcgacgataa cgtcacttta
     9841 aatctggaca tcctaaagtt tggaacaacg tatgaaccag ttcctccttc agcagattcg
     9901 tcgaaggcat atcttgcgag aggcggaggg gtatcttgaa ttggggctat tcgaaagggc
     9961 cttggagtgc ctagatcgtt tgccagatag ggatcgtcaa tcttttgagg cccgtctcat
    10021 ccgcgccgag attcttcgtg caatgggaca gtacgagtcg gcgctggaga tttacgaaag
    10081 cctcattgcc cagcggccag atgacctgaa cattttgttg gcgatgggct ggtgccataa
    10141 acgaatcgga cgcatcgatc ttgcttgcgc cgacctggaa aaggcaaaac agattagtcc
    10201 tcgcaactcc ttagtgctct acaatttggc ctgctattat agtctcgcag ggcgaaagca
    10261 ggacgccctg actcacctcg aaaaggctct ccaactggaa cctgggctgc gcaaactcat
    10321 cgacacagaa acggattttg atcccttgcg atctgattcc gatttcatcc ggctggttta
    10381 ccccgataag ggaaaccgct aaattgtctg gtgtcggtca agcaatcgtc gtagcggtgg
    10441 aggctgccat gcactgcagc caccgctgct tgtggtgcac cagcggccca tgggttttca
    10501 ttatttaaga ttggagcgat ttgccatggc acgttctttc aggcaggcgg cggtttcccg
    10561 acgttttctg gcaggcacag gcggaagggt cactcggcga aatctgatta aaggcttggg
    10621 ggctgcggtc acacttccct ggataggaaa ggccttggct gctctctcac cgaaggctga
    10681 gatcctcgaa gttaaaacga tcagtcgttt gccccaccgg taccacggct ggcccaccgt
    10741 ggcaagacgc aaaaatggtc aactcgtctt ggtatattcc ggtggtcgcg aggcccatgt
    10801 atgcccgttc ggtcgcgtgg aaatgatgac atcaaacaat gacggcgatt ggtggacata
    10861 cccacgggtt ttgatcgatt cggccattga tgaccgagat gccggcgttc tcgaaacggc
    10921 aaagggaaca cttctcgtca cgacctttac ttcgctagcg tacgtgccgg tcctggaacg
    10981 ggcgatggcc gatccgggca agggctggcc ctctgagaaa atcgatcaat ggcgggcggc
    11041 tcacgaacgg ctgacggagg cccatcgcaa gtcacaactt ggcgtgtgga tgatccgctc
    11101 tgaagacggt ggtgtgacgt ggtcgtctcc gtacgactgc ctcattgaca gtccccacgg
    11161 tcctattcag ttgcgggatg gccgcctcct ttacgccggg aaagaccttt ggcgtacaga
    11221 gaacgttggt gtctgcgtct cggaagatga cggacggacc tggcagtggc ttgcagtctt
    11281 gccaactcgg gaaggtgaca atcccaagca gtaccatgaa cttcacgcgg tggagacgac
    11341 aagtgggcgg ttaatcgtgc agattcgcaa tcataattcc gcaaacagtg gcgaaaccct
    11401 gcaatcggaa tcggaggacg gcggaaagac atggagcgtc cccc
//
LOCUS       DAOSUT010000131        11245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3266_length_11245_cov_9.630116, whole genome shotgun sequence.
ACCESSION   DAOSUT010000131 DAOSUT010000000
VERSION     DAOSUT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_11245_cov_9.630116"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="PLD05_11700"
     CDS             complement(<1..2099)
                     /locus_tag="PLD05_11700"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOP78148.1"
                     /translation="MKSASWLVINLLVICAVICGPQPTIGAETTLRLDPPVGPQQKPY
                     SPADDQVVEINPPPFIWVPAGKGVTYALQLSRSETFTEEPVRTYGNLHRSVFVPQEVL
                     PPGQWFWRYGIETEKGIVWGRARPFRIPEDARAFPFPTWDQIISKIPRDRPRLFFPGA
                     RLEEVRRLAAGPLKSQVNRLVDSCRRRVGEPLVPEPGYAPTGADRGPWAVNVMRTTRP
                     PMDVMESCALAWLLTGDHDLGQEAKRRILHFFGWDPNGPTSLFAYDEPAMWMMMRGTR
                     AYDWTYELFTPEERQRIESVMKIRAKQFLDRLQRMPFESNPYESHAGRLPGFLGECAL
                     SFIHEWPEAREWLDYATLLYYTSYPAWGGDDGGWQEGPAYWSAYMQFALHYVVALRAA
                     TGIDLMQKPFFRNTPYYALYTATPYHQHMPFGDGQTGNPRGLGHVMYVFSSLTQNPYF
                     RWYAEECGTTIGTDVLSLATYDPGLTARSPKELPQARAFPSVGLASIHTALGDRENDI
                     SFLLRSSPYGSVSHGHADQNAYVIEAFGRGLAIATGYYPWYGSPHHDRWTRSTRAVNS
                     ILVDGQGQVQRRWGMGGELVHFASTDGYDYVEAEAAAAYGGRLDRFRRHVVHIRPGVF
                     VMFDELQAPQPSQFQWLLHAYNQIKIDETRQVLNITNEPAALQVHLLLPEKLEFSQTD
                     RYDPEPEPIARDWQNTW"
     gene            complement(2613..3800)
                     /locus_tag="PLD05_11705"
     CDS             complement(2613..3800)
                     /locus_tag="PLD05_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78149.1"
                     /translation="MQTINVALIGQGFMGRSHSNAWGQVAKFFKPPIKPIMHTSFGQK
                     GVDPDPEGFAANWGWKNVSTDWESVVKNPEIGLVDIVTPNYMHAPVAKAAIAAGKHVA
                     CEKPIAGTLADAREMVQAAKKANVKTFVWFNYRRCPAVALAHLLVKQGKLGEIRHVRA
                     AYLQDWADENVPLVWRFDKNLAGSGAHGDLNAHIVDMTRFVTGMEITEIAGAIAETFI
                     KKRKLPSAGSTGAIVSGGAGKEEWGDVTVDDTVLFLARFSNGAVASFEAARQATGNQN
                     RNMFEINGTKGALKFDFERMNELLFYDATLPRAVQGWTNIMVTHGADHPYVANWWPDA
                     HIIGYEHGFVNQAYDILKVIAGEEPTVPIPDFEDAYQTQRVLEAALISATERRPVKLD
                     EVY"
     assembly_gap    3882..3981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3982..4629
                     /locus_tag="PLD05_11710"
     CDS             <3982..4629
                     /locus_tag="PLD05_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOP78150.1"
                     /translation="QQVQRLADRGKIPGRKVAGQWRFSPAEIHQWLEHRIGLLTDDED
                     FAEMESQLKPKNATAGEISIAALLPVEAIQVPLVGRTRNSIVREIVEVAARTGWLWDT
                     QAMIEAVLAREDMHPTALENGVALLHPRRPQPRILEQPFLAFGRTQSGIPFGGDTGTL
                     TDCFFLICSVEDRGHLRTLARLSRLLAVPEFLPTLRELEDPSAIRKWIAEVEKGF"
     gene            5040..6827
                     /gene="ilvB"
                     /locus_tag="PLD05_11715"
     CDS             5040..6827
                     /gene="ilvB"
                     /locus_tag="PLD05_11715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOP78151.1"
                     /translation="MTTQPQTTGASLQLAKPGEPVTGAEIVVQSLVNHGVEVVFAYPG
                     GASMPIHQAFTRFRDKIRVILPRHEQGGGFAAQGYARSTGKVGVCMTTSGPGATNVIT
                     ALADAKLDSVPIVAITGQVGTSVIGTDAFQETPTVEVCRSITKHHYLVTDVNDLARVF
                     REAFYIAQTGRPGPVLIDIPKNIQLAQTVPDYNAPMDLPGYRPVVRLARPEEIREIAQ
                     VIKKSRRPVVYAGGGVITSGASEALREFVEKTSIPVAMTAMGLGGFPGDHPLSLDMLG
                     MHGSIYANYAVAECDLLLAFGVRFDDRVTGKVSEFAKHATIVHIDVDPSELNKVKPAH
                     IAVVSDLKYALEELNKIVEPPDISEWVTRVMERKKAEPFSYREYEDAILPQQAIHELW
                     KLTKDQDTIITTGVGQHQMWTAQFYKFRKPRTWISSLGLGTMGFGLPAAIGAKVAHPD
                     KIVIDIDGDGSLLMNIQEMATAYCEKIPVKVLLLDNQHLGMVVQWEDRFHARNRAHTY
                     LGPIDQPEAVGRGEGKIPRYHYPDFVTIAKGFNWRARHIRHKSELIDGLKEMLASEEP
                     YLLDVLVPYQEHVLPMIPAGMTIKELIRE"
     gene            complement(7104..8777)
                     /locus_tag="PLD05_11720"
     CDS             complement(7104..8777)
                     /locus_tag="PLD05_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78152.1"
                     /translation="MKSFLVLTVPATVICVLGWLFSIASAEDASQQPVAISTFHCIGL
                     YWSPPGGSPDRQVLVRFRRQGEEAWQQGLPMRYNPIPKTDQDLADYRGSLVHLTPGTK
                     YEIELQLEGTDQVARLVASTWSEEFPIGEVINVPGGKEPLAITESGRPDAYRVYDGQG
                     AVIDVEHKHDTCISIDASYVIVRNFVLKGAGATNNPRGSPIGAIRIDKGHDIVIEDCD
                     ISDWGRTNPKTGFGFDYDAAVLCRNRDVHRVIIQRCRMHHPFSDTNTWYEPVYPTHPQ
                     GPQCISFFDTAGNHVIRYNEMYSDLDHMYNDIVGGGSNGSYRGSPGHDSDIYGNIVSH
                     CWDDGLEVEGGNRNVRVWGNYIRQCAMMIGNAPTSIGPLYIWGNVVERSQWRPDYTGG
                     SFLKMGFAGSEDWMTGHMYIFHNTIFAGDPLLPTGGFGGNRIVKHTVSRNNILHVRSP
                     DHRSISSAPQNVDNDFDYDLFNGRVPEDHEKHGIRGVPRYVAQAGFDPETKTGIFQLE
                     DTSPGAGAGIVIPNFTPPFVGKAPDVGAHQRGAPPMRFGISAAVAASSR"
     gene            complement(8985..>11245)
                     /locus_tag="PLD05_11725"
     CDS             complement(8985..>11245)
                     /locus_tag="PLD05_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HOP78153.1"
                     /translation="PRPLSRRTGEGRLWDRLFWCAHGADAPWYRQHQQALLDGVDEVV
                     IDPGAVPGPLCVTGEKAFPVLVGTTGNGYQPVVVAGEWGTERVVAFGHTGAFWTEKAL
                     SAPGTRRFLLNAIQWASSGAVPCNVAIYRMSNLADWLRNDDESRRRGIRVMDWKQLNP
                     AFLSQTHVLILNSHDLTPATLPVVSNYVRMGGGLLVGGLGWGWLQLNPGKNLRDDFPG
                     NLLLADCGIFWADGYLQRTTRMGFASSPLPGAEFHALEAAKLLGWGKTPNNDVPDEVI
                     EQAAQTVTLALSVVPQNHPLRQAMSRGKGLSSVAVIPTPQSPLRQSDKAARAMLQLQV
                     RQSLDAEANMVKAHPAAVVFPGQPPTSARTVRKSITIDPTHTGWQSTGLYAVAGRPIT
                     VTFDSPDVANQVFVQIGCHTDQLWHLPEWRRVPEVVKRVTVQGPRVTLASPFGGLIYL
                     GMVRARSQMGTIRCQIEGAVEAPMYVHGQTNARDWQRIRLAPAPWAELVSDRIILTLP
                     SEYVRSLEDPAQIMEFWTAVIAACNRLAGGPARTDAQRIVCDVQISAGYMHSGYPIMT
                     HLDIAPVLVNVDRLRRNDHGGVWGLFHEIGHNYQSPDWTFEGTGEVTNNLFTVYVFDH
                     LCAVPREKTHPALATRERLERRRKYFSAGSPFEQWKNDPFLALDMYLQLQEAFGWEPF
                     EKTFAAYRTLKPEERPKSDAEKRDQWVMRFSRAVNQDISPFFQQWGIPLSPEAVQSLS
                     DLPAWQPLILSK"
BASE COUNT         2414 a         3270 c         3131 g         2330 t
ORIGIN      
        1 tgccatgtgt tttgccaatc gcgggcaatc ggctccggct ccgggtcata acgatccgtt
       61 tgagaaaact ccagcttttc cggcaagagg aggtggacct gaagtgcggc gggttcattc
      121 gtgatattca ggacctgcct cgtttcgtcg atcttgattt gattgtatgc atgcaagagc
      181 cattgaaact gcgaaggctg aggtgcttgc aactcgtcga acatgacaaa gacacctgga
      241 cgaatatgca ccacatgccg ccggaaacga tccagacggc caccgtaagc cgcggctgcc
      301 tctgcctcaa cgtagtcata gccgtccgtt gaggcaaaat ggaccaactc tccgcccatt
      361 ccccatcgtc gctggacctg cccttgtcca tccacgagga tactgttcac cgccctggtc
      421 gatcgggtcc agcgatcgtg atgaggtgat ccataccagg gatagtatcc cgtcgcaatg
      481 gccaagcctc gcccaaaagc ttcgatgaca taggcgtttt ggtcggcatg cccatggctg
      541 acactaccat aggggctgct tcgcaaaaga aaactgatat cattctcccg gtcgccgagg
      601 gccgtatgaa tggaggccaa accgacgctc ggaaaagcac gtgcctgtgg taactctttg
      661 ggtgaccggg ctgttaagcc ggggtcgtag gtggccaggc ttaacacatc cgtgccaatc
      721 gtcgtgccgc attcctccgc ataccaacgg aaatagggat tctgcgtgag agaggaaaaa
      781 acgtacatga cgtgccctaa accccgcggg tttccggtct gaccatcgcc aaagggcatg
      841 tgttgatggt agggcgtggc cgtgtagagg gcgtaatacg gtgtgtttcg gaagaagggt
      901 ttttgcatta ggtcgatccc ggtggctgcc cgcagtgcca ccacataatg caacgcaaac
      961 tgcatgtaag cgctccagta tgcgggacct tcttgccaac cgccatcatc gccaccccat
     1021 gccggatagg aggtgtaata cagcaaggtg gcatagtcga gccactctcg ggcttcaggc
     1081 cattcgtgaa taaaagaaag cgcacattcg ccaaggaagc ccggcaaacg tccggcgtgg
     1141 ctttcatacg ggttgctttc aaacggcatc cgctgaaggc ggtccaggaa ttgcttggct
     1201 cggatcttca tgacggattc gatccgctgc cgctcctccg gcgtgaacag ctcgtacgtc
     1261 cagtcgtaag cccgcgttcc ccgcatcatc atccacatgg ccggttcgtc ataggcgaac
     1321 aggctcgttg gaccgttggg atcccaaccg aaaaagtgca ggatgcgacg ttttgcttct
     1381 tgacccagat cgtggtcacc tgtcaacagc caggccaacg cacaagattc catcacatcc
     1441 atcggcggcc gtgtggtccg catcacattg acggcccagg gcccgcgatc cgcgcccgtc
     1501 ggggcgtagc ccggttcggg caccaatggc tcgccgacac gccgccgaca tgaatcaaca
     1561 aggcgattga cctgggattt cagcggtccc gctgccaggc gtcggacttc ctccaaacgt
     1621 gccccgggga agaacagacg cggtcggtct cgtggaatct ttgaaatgat ctgatcccag
     1681 gtggggaatg ggaaggccct ggcgtcttcc gggatacgaa atggccgcgc tcgaccccaa
     1741 acgatgccct tttctgtctc gatcccatat cgccaaaacc actgtccggg cggtaaaact
     1801 tcctgcggga caaagacgga tcggtgaagg ttgccgtatg tgcggacggg ttcctccgtg
     1861 aacgtttcgg aacgggagag ttgcaaggcg taggtgaccc cctttcccgc ggggacccaa
     1921 ataaagggcg ggggattgat ttcgacaact tgatcatctg ctggtgaata gggtttttgc
     1981 tggggtccca ctggaggatc cagccgcagt gtcgtttcgg ctccaattgt aggctgtggt
     2041 ccacaaatga ctgcgcagat gaccaacaaa ttgataacaa gccaagatgc gcttttcatg
     2101 gaaggtctcc tcggttggct aaggctttct aacccgacac gtccaccctg aggtaatctt
     2161 tcggcaggaa ccggttgctt gctgatcacc atcgttaagg gtcgagaggt ggctgtcaac
     2221 agggcgttca agccgcaggg atgtgcacgt ccgctcgtgg ctctgggaag gatcgtagat
     2281 catccattga cactaagagc ctaccctgta atctcccctc tcccgttcag cgggagaggg
     2341 attgggggtg ggggtgactt gaatcgctgc cggttttctc agtagattcc tacgatcgct
     2401 tgccttcagc ccaaccctct cccagaggga gagggggagt ttttggtatc cgctttaagg
     2461 caggcgcgac ggcgcttccc tccagatcag gcgcaaaaaa gcggcgcctt ggatgctcgc
     2521 tctccgcggt ccaaggcgcc ggaaaatgcg acttcctgtc ggcacagaat ttcacaaacg
     2581 acaggtgtct tccgcggtcc tcgccgtttc gatcaataga cctcgtccag tttcacaggt
     2641 cgccgttcgg ttgccgagat caacgccgct tccaggaccc gctgtgtctg ataagcatcc
     2701 tcaaagtccg gaatgggcac ggtcggctct tcgcccgcaa tgaccttcaa aatgtcgtac
     2761 gcctgattca cgaaaccgtg ttcgtagccg atgatatggg catccggcca ccaattcgcg
     2821 acgtagggat gatcggcccc gtgggtgacc atgatgttcg tccaaccctg cacggcacga
     2881 ggcagcgtgg catcatagaa taacagctcg ttcatccgtt caaaatcgaa cttaagagca
     2941 cccttcgtgc cgttgatttc gaacatgttg cgattttggt tacccgtcgc ttgacgtgcg
     3001 gcctcaaagc tggccacggc gccgttggag aagcgggcaa ggaatagcac cgtgtcatcg
     3061 actgtgacat caccccactc ctcttttcca gcgcctccgg agacaatggc ccccgtcgaa
     3121 ccggcactgg ggagcttgcg cttcttgatg aaggtttcag caatggcgcc ggcaatttcg
     3181 gtgatctcca tgcccgtgac aaagcgggtc atatcgacga tatgggcatt gaggtcaccg
     3241 tgggccccag acccggcgag attcttatcg aaccgccaca caagaggcac gttctcgtcg
     3301 gcccaatctt ggagataggc ggcccgtacg tgacgaattt cgccgagctt gccctgcttg
     3361 accaaaaggt gggctaacgc gaccgccggg cagcgacgat aattgaacca gacgaatgtc
     3421 ttcacgttgg ccttcttggc ggcctggacc atctcgcggg catcggcgag cgtccccgca
     3481 atcggctttt cgcaggccac gtgcttgccc gccgcaatcg ctgccttggc cactggggcg
     3541 tgcatgtagt tgggtgtgac gatatcgacc aagccgatct cagggttctt caccactgac
     3601 tcccaatccg tggaaacgtt cttccaaccc caatttgcgg cgaagccctc tggatcgggg
     3661 tccacgccct tctggccaaa ggacgtatgc atgatcggtt tgatcggcgg cttaaaaaac
     3721 ttggccacct gaccccaggc attggaatgg gaccgaccca tgaacccctg accgatcagc
     3781 gcgacattga tcgtttgcat ggcaagctcc tccgaatgtt gataactctt gtgaccttgt
     3841 ttgataagcc ctttggaccg ccgttatatc gcattttgct annnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn ncaacaggtg caacggctcg ccgatcgggg gaagattccc
     4021 ggacgaaagg tggccgggca atggcgtttt tcaccggcgg aaatccatca atggttggaa
     4081 catcgtatcg gattattgac cgatgacgaa gatttcgccg aaatggaatc gcagctcaaa
     4141 ccgaagaatg cgacggcagg cgagatttcc attgccgccc tcctgccggt cgaggccatt
     4201 caggtcccct tggtaggccg cacgcgaaat tcaattgtcc gtgaaatcgt ggaagttgcc
     4261 gcccggacag gctggctctg ggacacccag gctatgatag aagcggttct tgcccgcgaa
     4321 gatatgcacc caacagcctt ggaaaacggc gtggcccttc tgcacccgcg ccgtccccag
     4381 ccgcggattc tcgaacaacc gtttctggcc tttggccgaa cgcaaagcgg tatccccttc
     4441 ggcggcgata ctgggacctt aaccgactgt ttctttctca tctgttcagt ggaagaccgt
     4501 ggccatctgc ggaccttggc ccgcttgagc cgcctgttgg ccgtccccga attcctgccc
     4561 accttgcgcg aattggaaga cccgtcggcc attcgcaagt ggatcgccga agtcgagaag
     4621 ggattctgat cccgcgcgaa ccaaagtgat cggttggcaa tctctaaccg cagggccccc
     4681 ggcatcggca ataatgtatt tatggacttc ggcgtgaagg gttgccgaac ctggatcatg
     4741 ccttcgttga aaaaaggcag tattatccgt atgctgaggg atgggcagag gattggacac
     4801 gagcaaaggc ttaaagaaat cggcatttta tttcaaaaaa cttgacaatc ggcgacttgc
     4861 ggtcctggcc gcacgctttg agatgattct tcccccgttg tgggtattaa gaaggggctg
     4921 attgagtgcg cggtagaata gaccaagcgg ccgcggcata gttgttaggc taaggcatcg
     4981 gcatcacaga acgcgtttct gcattgtcgg agaaagtttt aggagaactg agtgtgacga
     5041 taaccaccca gcctcaaacg accggagcga gcttgcaatt agcaaaacct ggcgaaccag
     5101 tcactggggc ggagattgtt gttcaatcgt tggtcaacca cggcgtggag gtcgttttcg
     5161 cctacccggg cggggccagc atgcccatcc atcaggcatt tacgcggttc cgcgacaaga
     5221 tccgcgtcat cctgccgcgt cacgagcagg gcggtgggtt cgctgcgcag ggatatgcgc
     5281 gatccacagg aaaagtgggc gtctgcatga ccaccagcgg tcccggggcg accaacgtca
     5341 tcacggccct ggctgacgcc aagctcgaca gcgtgcccat tgttgccatt acgggccagg
     5401 tgggaacctc tgtcattggg acggatgctt tccaggaaac gccgacggtc gaggtctgcc
     5461 ggagcatcac caaacatcac tacctcgtca cggatgtgaa cgatctggca agggtcttcc
     5521 gcgaagcctt ctacattgcc cagacggggc gacccggacc ggtgcttatt gatatcccca
     5581 agaacattca actggcccaa accgtgcctg actacaacgc ccccatggac ttgccgggtt
     5641 accggccggt ggtgcgattg gcaaggccgg aagagatccg cgaaatcgcc caggtgatca
     5701 agaaatcacg ccgtcctgtg gtgtatgcag gaggcggcgt gatcacctca ggggcttcgg
     5761 aggccttgcg cgagtttgtc gagaagacct cgattcctgt ggcgatgaca gccatggggc
     5821 taggtggttt tcccggcgat catcccctgt cccttgatat gctgggaatg cacggcagca
     5881 tttacgccaa ttacgcggtg gctgaatgcg acctcctgct ggccttcggc gttcggttcg
     5941 atgatcgcgt aacgggaaag gtctccgagt ttgctaagca cgccacgatc gttcacatcg
     6001 acgtggatcc gtcggagttg aacaaagtca aacctgccca catcgccgtg gtgagcgatc
     6061 tcaaatacgc cctggaagag ctgaacaaga ttgtggaacc cccggacatt tccgagtggg
     6121 tcacccgggt catggagcgc aagaaggccg aaccattctc ttatcgcgaa tatgaggatg
     6181 ccatcctccc acaacaggcc atccacgagc tttggaagct caccaaggac caggatacga
     6241 tcattaccac gggggttggc cagcatcaga tgtggacggc ccagttttat aagttccgca
     6301 aaccacggac gtggatctcc agtctgggat tgggcacgat gggctttggc cttccggccg
     6361 ccatcggtgc taaggtggct catccagata aaatcgtcat cgacatcgac ggggacggta
     6421 gcctgcttat gaatatccaa gagatggcaa ctgcctattg cgaaaagatt cccgtcaagg
     6481 tcctcctcct cgacaatcaa cacctgggca tggtggtgca atgggaggat cgtttccacg
     6541 cccgcaatcg tgcccatacg tatctcggcc cgatcgacca acctgaggcc gtcggccgcg
     6601 gagagggaaa gatcccccgc tatcattacc ccgatttcgt gacgattgcc aaggggttca
     6661 actggcgggc acggcacatc cgccacaagt ccgaactcat tgatggcctg aaagaaatgc
     6721 tggccagcga ggaaccgtac cttttggatg tgctggttcc ctaccaagag cacgtcctgc
     6781 ccatgattcc cgccgggatg acgatcaagg aactcatccg cgagtgatga cccgccttcg
     6841 cacatggggc accggcagca acaactgttt acggggaatg taggatcttc ccgaaagaca
     6901 cccgtaaggg gagggtcgcc gtcctcggcg accggagatt ttcatcgtcc tcagcgaccg
     6961 cagaatcggg agggtcgccg tcctcggtga cctcaggccg gccaccgtgc tgtccttggc
     7021 gaccaaatcc tcggacggcg gggacgccgt ccctcccttt tgttcgccca ctctgcagag
     7081 tgtagttcta aaacaggcga ccgtcaccgg gatgacgcgg ccaccgcggc ggaaataccg
     7141 aaccgcatgg gcggcgcacc ccgctgatgt gcccccacat caggcgcctt ccccacgaaa
     7201 ggcggagtaa agttcggaat aacgatgccc gcgccagcac ccggactggt gtcctcaagt
     7261 tggaagatgc cggttttcgt ctcggggtcg aacccagcct gggccacata ccggggcaca
     7321 ccgcggatac cgtgtttctc atggtcttcg gggacccgtc cgttaaacag gtcatagtcg
     7381 aagtcgttgt ccacattttg aggggccgag ctgatgctgc ggtgatccgg cgagcgaacg
     7441 tgcaagatat tattgcggga aactgtgtgc ttaacgatgc gatttccccc gaagccgccg
     7501 gtgggcagca gcggatcacc ggcaaagatt gtgttgtgaa agatgtacat gtgaccggtc
     7561 atccagtcct cgctgcctgc gaagcccatc ttgaggaaag agcctcccgt gtagtcgggc
     7621 ctccactgac tcctttcaac cacattcccc cagatataga gcggaccgat tgaggtaggg
     7681 gcgtttccga tcatcatcgc gcattgtctg atgtaattcc cccaaacccg cacgttccgg
     7741 ttgccaccct cgacttccag gccatcgtcc cagcagtggc tgacgatatt gccataaata
     7801 tcagaatcgt gacctggcga accgcggtag ctgccattgc ttcctccgcc cacgatgtcg
     7861 ttatacatgt ggtcgaggtc ggaatacatt tcgttgtacc ggatgacatg atttccggcg
     7921 gtgtcaaaga aggagatgca ctgcggacct tggggatgcg tgggataaac cggctcgtac
     7981 cacgtgttcg tgtcggagaa aggatgatgc atccggcacc tttggatgat caccctgtga
     8041 acatcgcggt tgcgacaaag aacggccgcg tcatagtcga atccgaagcc tgttttcgga
     8101 ttggtccgcc cccagtcgga aatgtcgcag tcctcaatga caatgtcatg ccctttatca
     8161 attcgaatgg cgccaatggg agatccgcgc ggattgtttg tcgcccccgc gcctttgagc
     8221 acgaaattcc tcacaatcac ataagaggcg tcgatgctga tgcacgtatc gtgcttgtgc
     8281 tctacatcaa tgacggcccc ttgaccgtcg tacacgcggt aggcgtccgg ccggcccgat
     8341 tcggtaatgg cgagcggttc cttcccgccg gggacgttga tcacctcacc gatgggaaac
     8401 tcctccgacc acgtagaggc gaccagccga gccacttgat ccgttccttc cagttgtaac
     8461 tcgatctcgt acttggtgcc cggtgttaaa tggaccaaac tgccccggta gtcggcgaga
     8521 tcctgatccg tcttaggaat cggattgtag cgcatgggta ggccctgctg ccaagcttct
     8581 tcaccctggc ggcgaaaacg caccaaaacc tgccgatcgg gtgatccccc gggcggcgac
     8641 cagtaaagac caatacagtg aaacgtggaa atggccacgg gctgctgcga ggcatcttcc
     8701 gccgaagcga ttgaaaacag ccagcccaga acacaaataa cggtcgccgg cactgtcaaa
     8761 acgagaaagc ttttcatccc gtgtgcctcc ccaaccaaat gccacatctc ttcacgaact
     8821 gccatccgat cttacacccg gagacacgac acgtagccca ttatgtgcca gtcaacctcc
     8881 gttgaacatc cttagcgatg atgaacacgc tgtcatcaga tcgcgccttt tgcgaagcgt
     8941 gccacttgac gcaacaatgt taccaagtgg atcaccgcgc atgatcactt ggacagtatc
     9001 aatggctgcc aggcgggcag gtcactcaac gactgaacgg cctcgggcga caggggtatc
     9061 ccccattgtt ggaagaaggg gctgatatcc tggttgacgg cacgagaaaa acgcataacc
     9121 cattgatcgc gcttctcggc atcacttttc gggcgttctt cgggtttcag tgttcgatag
     9181 gcggcaaatg tcttttcaaa gggttcccag ccaaacgcct cttgtagctg taaatacata
     9241 tccagtgcca gaaatggatc atttttccac tgctcaaacg gactacctgc cgaaaagtat
     9301 tttcgccgcc tttcgagtcg ctcgcgcgtg gccagcgccg gatgagtctt ctcgcgtgga
     9361 accgcacaga ggtgatcaaa aacatacact gtaaacagat tatttgtgac ttcgcctgtg
     9421 ccctcgaagg tccaatccgg tgactggtag ttgtgcccga tttcgtgaaa cagtccccac
     9481 acgcccccgt gatcgttccg ccgtagccga tccacattca ccagcacagg ggcaatgtcc
     9541 agatgcgtca tgatgggata accggaatgc atatatcccg cgctgatttg gacgtcgcac
     9601 acaattcgct gcgcatcggt ccgggctggt ccgcccgcca gacgattgca ggccgcaatc
     9661 acggctgtcc aaaactccat gatctgggcg gggtcttcca atgaccgcac atattcggac
     9721 ggcaaagtga gaataatgcg atcagagaca agctccgccc acggagccgg ggccagccga
     9781 atcctctgcc agtccctggc gttggtctga ccgtgcacat acatcggggc ttctactgcc
     9841 ccctcgatct ggcacctgat ggtacccatc tgcgaccgag ctcgaaccat tcctaaatag
     9901 atcagcccac cgaacggact ggccagggta acgcggggac cctgtaccgt gacccgttta
     9961 accacttccg gcacccgtct ccactctggg agatgccaga gctggtcggt gtgacagcca
    10021 atttgtacga acacctggtt ggccacgtcg ggagaatcaa atgtgaccgt gatcggtcgt
    10081 cctgccacgg cgtacaaccc cgtgctttgc cagcccgtgt gtgttggatc aatcgtgatg
    10141 cttttgcgaa cggtacgggc agaagttggc ggctgcccgg gaaacaccac agcagcggga
    10201 tgggccttca ccatattcgc ctcggcgtca agcgactgcc gcacttggag ctgaagcatc
    10261 gcccgtgccg ccttgtccga ctggcgaagt ggcgactgcg gagtcggaat gacggcaacc
    10321 gaagaaagac ctttccccct cgacatggcc tgtctcagcg gatgattctg aggaacgacc
    10381 gacaaggcaa gcgtgacagt ttgggcagcc tgctcgatca cctcatcggg aacgtcattg
    10441 ttgggtgttt tgccccaacc caagagtttg gccgcttcca gggcgtgaaa ttctgctccc
    10501 ggtaaagggg aactcgcgaa ccccattcgg gtcgttcgtt ggagataacc atccgcccaa
    10561 aaaatgccac agtcggccaa caggagattc ccgggaaagt catcccggag attcttgccg
    10621 ggattgagct gcaaccagcc ccagcccaat ccaccgacca atagacctcc acccattcgc
    10681 acataattgc tcacgacagg gagagtggcc ggcgttagat catggctatt caggatgagg
    10741 acatgagttt gagaaagaaa cgcggggttg agttgcttcc aatccatgac gcgaatgccc
    10801 cggcgacgcg attcatcatc attgcggagc caatcggcca ggttagacat ccggtaaatc
    10861 gccacattac acgggactgc ccccgagctt gcccattgga tcgcattgag caagaatcgc
    10921 cgcgtgcccg gcgcgctcag ggccttttcc gtccaaaaag cgcctgtgtg tccgaaggca
    10981 acgactcgct ccgtgcccca ctctcccgcg accactaccg gctgatagcc gttgcctgtc
    11041 gtacccacca gaacgggaaa cgccttttcg cccgtcacac aaagaggccc tggcaccgcc
    11101 cccgggtcaa taacaacctc gtcaacacca tccaggagtg cctgttggtg ttgccgatac
    11161 cacggggcgt ccgcgccgtg ggcgcaccaa aagagcctat cccacaacct cccctctcct
    11221 gttcggcggg agaggggccg ggggt
//
LOCUS       DAOSUT010000132        11217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3276_length_11217_cov_9.702114, whole genome shotgun sequence.
ACCESSION   DAOSUT010000132 DAOSUT010000000
VERSION     DAOSUT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3276_length_11217_cov_9.702114"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLD05_11730"
     CDS             complement(<1..653)
                     /locus_tag="PLD05_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOP78154.1"
                     /translation="MFWLMDLVYFVLLLVTAPIWVTRLVLHRRKYQHGWAEKLLGLVP
                     CRNSNAPCIWFHAVSVGEVNLLATLLAEMRRRLPNYDICLSTTTQTGMEVAKKKYPEL
                     LLFYCPMDFSWAVRRAMRRIRPNLLVLAELELWPNLIRAADEYGARVAVVNGRLSDRS
                     FRGYQRIRWVFAKTLRRIALVAAQDPTTARRFIELGVPPDRVAVTGSLKFDGTEFDR"
     gene            complement(765..4469)
                     /locus_tag="PLD05_11735"
     CDS             complement(765..4469)
                     /locus_tag="PLD05_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOP78155.1"
                     /translation="MELLERIWEIVSDTFVVVGQAVDRGITSLFGSANARYLRRLQPK
                     VQAINDLEPRYQRMTDAELREQTAKFRKRLEAGETLDDLLVEAFAVCREASRRVLGMR
                     HFDVQLMGGMVLHAGAIAEMVTGEGKTLVATLPAYLNALEGKGVHVVTVNDYLARRDM
                     EWMGPLYMFLGLTVGAIQHDMGARERQQAYSCDITYGTNNEFGFDYLRDNMRLAAKGD
                     NRYPPEYQQVQGRLHYAIIDEVDNILIDEARTPLIISGPAHDDISRYKRADQIARQLI
                     RDVHFVVNEKDHTCHLTEEGIRKAEELAGVESFYTAGNLEWPHLIDNALKAHHLYKRD
                     VHYVIENNQVIIVDEFTGRLMYGRQWSDGLHQAVEAKEGVPIKEETQTLATITIQNFF
                     RLYDKICGMTGTAMTEANEFWKIYKLEVVAIPTNKPLRRINHPDVIYRTAREKYKAIV
                     DEIERMHKWDTVWYSRYEYEVGRIVEEDDERVVFVTREGDRKVIPRSQIHNIEKKGRP
                     ILVGTVSVEKSEMLSEMLDRRGIPHQVLNAKHHAREAEIIAQAGRKGAVTIATNMAGR
                     GTDIILGGNPEAKAWSILQHKYRSRLDVPRDEWDALVKEIEEKEQMKAEGREVAALGG
                     LHVIGTERHEARRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFAGDWVKNILTRLG
                     MEEGQAIESPMVTRRIEAAQKKVEERNFEIRKSLLEFDQVMDEQRKRVYGYRQSILEG
                     ADCRSLIRDMIRQEIDKNLDRFLDKSYGAETFAQWISKQLSVEFRARDFRGMDFPTAE
                     AYARDHAERMAESQIFDLIEENLPEEAERTDWNWEALTKAVNTRWRLDLKDRELRKIP
                     REDLSEYLIEKAQQSIRTIDLSEGAKYLDPDFGLKSAINWVRSKFGIELTVEELRDLE
                     PSAIKDLVFEKALKAYQQKEIEFPVRAAFSHYTGRDATGRRVYDREGIIRWAKGRLGV
                     ELTEEDLRTKTGQDIWNLLVEASEKDYLKAEALRTEGEALLASLYEGLGDDPRVKCDR
                     NHPKLGEFCRWVAEHLGLEMTPESVARMERRDLQWKFRDAFNDRYSPEMRQMERFLLL
                     HTLDSAWKEHLLVMDHLRSSVSLRGYAQVDPKVEYKREGMRQFELMWESVGRRVTDLV
                     FRMEQMDERFVGSMWKETAAERPEASPTVTASVATLGSQQQEAIENSQAGSKAKPIRK
                     SGPEIGRNDPCPCGSGKKYKHCCMRNRRPA"
     gene            complement(5159..6061)
                     /locus_tag="PLD05_11740"
     CDS             complement(5159..6061)
                     /locus_tag="PLD05_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOP78156.1"
                     /translation="MSRPRATSSGRTVTGTASQTKTPIAKPQNSSKDRSIREESMPTT
                     IAPEDVEQLWIEFKKNPQNQELRNRLVEIYLPLVKYNAERIWARLPEGVDLDDLISAG
                     VFGLMDAIDAFDLSRGVKFETYCVPRIRGAMLDELRTMDWVPRLVRSKASKLNEACKT
                     LEARLGRQPTDSELAQELGLTIEELEKMKTEASAINLISLNKKRYETDSYKDVREIDI
                     LEDKKGEDPTRRIEKSDLMRLITKGLNRNERLILILYYYEELTMKEIGATLDLSESRV
                     SQMHSAIVQRLQQQLAKRRAELGT"
     gene            complement(6171..6428)
                     /locus_tag="PLD05_11745"
     CDS             complement(6171..6428)
                     /locus_tag="PLD05_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78157.1"
                     /translation="MARIYAVVFGSLGLCTALIRDMIHGVSPQDALLRGWIVLLFFSF
                     VGAAIGAVAEQVLREAVEQRLRSQMAEKLNGASESQAERNK"
     gene            complement(6721..7539)
                     /locus_tag="PLD05_11750"
     CDS             complement(6721..7539)
                     /locus_tag="PLD05_11750"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HOP78158.1"
                     /translation="MDQSGVMYDQAYHLRQLVSRIRPATVDDRQPTLQIRIYGVRQGV
                     GTSTVAEALAHYLHQVGWNVCLFNGVPKNLGKPPAPFRVEHHGQWVETAMSHGDREQE
                     ISTAFTTRRGREHLSGIESRKRLLLRDCGVANDPKELVSDSSRRCVLVTTPAPTDIMK
                     AYALVKRTTGVASSFLPTIVINKSAELSIGQEMAGRLQRACGRFLNRDLTSLFILPIL
                     PRTEGESVNAGEFDEGNWLRRTDGSAQHGSTYCCRREWEETIQLIAKALTEGVT"
     gene            complement(7611..8831)
                     /gene="flhF"
                     /locus_tag="PLD05_11755"
     CDS             complement(7611..8831)
                     /gene="flhF"
                     /locus_tag="PLD05_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOP78159.1"
                     /translation="MDAMDVRTFRASTMHEALAIVRRELGPDAAILRTREIRVRRLFG
                     LLPGRAEIEVTASVNVNVPSRLPPSSSPLGGLNRECRESVVETGLSLERASAETRALV
                     MRTGAFHPEVQHRLENMEQMLRTLCRRNNSSVPYDWPEHLFKVYTDLIDADLREEDAR
                     ELLERVRPESVRDRTDEPMLVKARLARILESELAATQPIRITPGRCRVCALVGPTGVG
                     KTTTIAKLAANFRLRENRRVALITVDTYRIAAVEQLRTYADIIDLPMHVVSTPREMRD
                     AVKDFTGFDLILIDTAGRSPRDEIKIRELRALLNEAHADEIHLVLSSVASPKTLLQTL
                     EHFRTVGPTALVLTKLDEAPSFGGLLPVLRSGGLPVSYLTNGQNVPDDIEIAQPSRLA
                     RLILGMDNVLRDPS"
     gene            complement(8914..11007)
                     /gene="flhA"
                     /locus_tag="PLD05_11760"
     CDS             complement(8914..11007)
                     /gene="flhA"
                     /locus_tag="PLD05_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOP78160.1"
                     /translation="MSAGDVGAKGIGRLIFKPQELIFPAAIIASLIVLLIPLPVWLLD
                     FLLAANITIAIIMLLTTIYVATPLEFSIFPSLLLATTLMRLVLNVATTRLILTRAGEA
                     GLEAAGSVIRAFGEFVAGDQIVVGLIIFIIIVVIQFVVITKGATRISEVAARFTLDGM
                     PGRQMAIDADLNAGIIDEREAQRRREEITRQADFFGAMDGASKFVRGDAIAGIIITLI
                     NIIGGLFIGMVQMGLPLSQAAALFTKLTIGDGLVSQVPAFLISLAAGLLVTRSSTEVN
                     LPAEFLRQMFGRPRTLAVAGAFLGILIFTQLPTIPLLTVGGACAALAVTLSRKTRKQK
                     QSEEARKQAEATKPPEERIEDYLLVDPMELEIGVGLIRLADPKRGGDLLDRIHRIRKN
                     IAGDIGIILPKVRIRDNFQLDQHQYTIKIAGAPVATGMVYAHLLMAMDSGMTTGKVPG
                     IETKDPAFGAPAIWIEPSQRERAEMYGYTVVEPAAVIATHLTETIRRHADEILTRDAT
                     KHLIEELKKSSPAVVEDLIPNVMRLAEVQQILQMLLREQVPIRQLGLILETLGDYAPR
                     TKDPILLTEFVRARLARTISTRYRDAEGRMLVVTLDPALEDRIRAGFDHTERGLFIRM
                     SPQMMERIARGIAQEVSKLTTQHRPPVVLVSPQIRPALKEMMTPYLPNLVVLSYNEIT
                     RDTRIESIAMVTDVA"
BASE COUNT         2378 a         3161 c         3000 g         2678 t
ORIGIN      
        1 ttacggtcga attccgtgcc atcgaatttc aaggatccgg tgactgcgac gcggtcgggc
       61 ggtactccca attcgataaa tcgtcgcgcc gtggttgggt cttgggccgc aaccagcgcg
      121 atccggcgga gagtcttagc gaagacccag cgaatccgtt gataaccgcg gaagctccga
      181 tcgctcagtc gtccattcac gacggcgacg cgcgccccgt attcatcggc tgcccggatc
      241 aggttgggcc agagttccag ttcggccagg acaaggagat tcgggcgaat ccgtcgcatg
      301 gcccggcgga cagcccagct aaaatccatg ggacaataaa agaggaggag ttccggatat
      361 tttttcttcg cgacctccat cccagtttgg gtggtggtac ttagacagat gtcataattg
      421 ggaagacgcc ggcgcatttc ggcaagcaac gtcgcaagca gattgacctc tccaacacta
      481 acggcatgaa accaaatgca tggtgcattt gaattgcggc aaggaaccaa accgaggagc
      541 ttttcagccc agccatgctg gtactttcgc cgatggagga ccagacgagt tacccaaatg
      601 ggtgccgtca caaggagtaa gacaaaatag acaagatcca tcaaccaaaa cattaggaat
      661 cctttccctc atggtttcaa accgaattgg gttcgatcgc tggaaggggt cacatggcag
      721 attcctcgct gactccaacc cgagaaaccc cggctgcaac cacactacgc cggccggcga
      781 ttccgcatgc agcaatgttt atacttttta ccgcttccgc aaggacatgg gtcatttcga
      841 ccaatttcgg gtccactctt gcggataggc ttggccttag aacccgcctg gctgttttcg
      901 atcgcctctt gctgctgact tcccagggtt gccacgctag cagtgacggt cggagaggcc
      961 tcggggcgct cggcggctgt ttccttccac atggagccta cgaatcgttc gtccatttgc
     1021 tccattctga ataccaaatc ggtaactcgt cgccccacag actcccacat cagttcaaac
     1081 tgccgcattc cttcccgctt gtattcgacc ttgggatcga cctgtgcgta accccgcagg
     1141 ctaacactac tgcggagatg gtccatcaca aggaggtgct ccttccaggc gctatcgaga
     1201 gtatgaagaa gcaggaatcg ctccatctga cgcatttctg gtgaatagcg atcgttgaac
     1261 gcgtcacgga acttccattg gagatcacgg cgttccatac gggcgacact ctcaggtgtc
     1321 atttcgaggc ccaagtgctc tgctacccac cggcagaact cgcccaattt aggatggttg
     1381 cgatcacatt tgacgcgagg gtcgtcgccg agcccctcat agagcgatgc caaaagggcc
     1441 tctccctcag tgcgaagggc ttctgccttg agataatcct tttcactggc ctccaccaag
     1501 aggttccaaa tatcctggcc cgtcttagtg cgaaggtctt cctccgtcaa ttcaacaccc
     1561 aaacgtccct tcgcccagcg aatgataccc tctcggtcat aaacccgtcg ccctgtggcg
     1621 tcgcggcctg tgtaatggct aaaggcagcc cgcaccggaa attcgatctc tttttgttga
     1681 taggccttca atgccttttc aaaaacaagg tctttgatgg cagaaggttc gagatcacgc
     1741 agctcttcga cggtgagttc aatgccaaat ttgctgcgaa cccaattgat ggctgacttg
     1801 aggccaaaat cgggatcgag gtacttcgct ccctcgctta gatcaatggt gcggatcgat
     1861 tgttgggcct tttcgatgag gtactccgac aaatcttctc gggggatttt tctcaactct
     1921 cggtccttga gatcgagccg ccagcgggtg ttgacagctt tcgtcagcgc ttcccaattc
     1981 cagtctgttc tctcagcctc ttccggcaag ttttcttcga tcaaatcaaa gatttgactt
     2041 tccgccatcc gttcggcatg atccctggcg taggcctcgg ctgtgggaaa atccatgccg
     2101 cgaaaatctc gcgcccgaaa ttcaacggac agttgcttgc tgatccactg ggcaaacgtc
     2161 tctgccccgt agcttttgtc cagaaaacgg tctaagtttt tgtcgatctc ttggcggatc
     2221 atatcgcgaa taagactacg acaatccgcc ccttccagga tgctttgccg atagccataa
     2281 acacgctttc gctgctcatc catgacctga tcgaattcga ggaggctctt gcggatttca
     2341 aagtttcgct cctcgacctt cttttgggcg gcttcaatgc gacgagtcac catggggctt
     2401 tcaatcgcct ggccttcctc catccctagc cgcgtgagga tattcttgac ccaatctccc
     2461 gcaaaaatcc gcatcaaatc gtcttctagc gagagataaa accggctact tcctggatcc
     2521 ccttggcgtc ctgcacggcc gcggagctgg ttgtcgatgc ggcgtgcctc gtgccgttcc
     2581 gtaccgatga cgtgcaagcc ccctagggcg gcgacctccc ggccttccgc cttcatctgt
     2641 tccttttctt cgatttcctt gactaaggcg tcccattcat cacgcgggac atctagccgg
     2701 ctgcgatact tgtgctgaag gatggaccag gccttggcct cggggtttcc accaagaatg
     2761 atgtcggtcc cacgcccggc catgtttgtg gcaatggtta ccgcgccttt acgcccagct
     2821 tgggcgataa tctcggcttc ccgcgcatga tgcttggcat tgaggacctg gtgcgggatg
     2881 ccgcgacggt ccaacatctc ggaaagcatc tcgctctttt cgaccgacac ggttcccacg
     2941 agaatcgggc ggcccttctt ttcgatgttg tggatttgcg atcgcgggat aaccttgcga
     3001 tcaccttctc gcgttacgaa cacaaccctt tcatcatctt cttcgacaat tcgaccaacc
     3061 tcatattcat aacgggaata ccaaacggtg tcccatttgt gcatccgctc aatttcgtcc
     3121 acaatggcct tgtacttctc tcgcgccgtc cggtagataa catcgggatg gttgatccgc
     3181 cgaaggggct tgttggtggg gattgccacg acctcgagtt tgtagatttt ccagaattcg
     3241 ttggcctccg tcatggcggt tccggtcata ccacaaatct tgtcgtacaa ccggaagaag
     3301 ttctgaatgg tgattgtcgc caacgtttgc gtttcttcct tgatggggac gccctctttc
     3361 gcctccacag cttggtgaag accatcactc cattgacgac cgtacatgag acgcccggtg
     3421 aactcatcga caatgatcac ctggttgttc tcgatgacat aatgcacatc gcgcttataa
     3481 agatggtgcg ctttgagggc attgtcgatt aggtggggcc attcaagatt accagccgtg
     3541 tagaaactct cgaccccggc cagttcttcc gcttttctga tcccttcttc ggtgaggtgg
     3601 cacgtgtgat ccttctcgtt gaccacaaaa tggacgtcgc ggatcagttg tcgagcgatc
     3661 tgatctgccc gcttgtaccg ggagatgtcg tcgtgggccg gtcccgaaat gataagcggg
     3721 gttcgcgcct cgtcgataag gatgttatcc acctcgtcga taatggcgta gtgaagacga
     3781 ccttggacct gctgatactc ggggggatac cggttgtcgc cttttgccgc caaccgcatg
     3841 ttatcccgta ggtaatcaaa accgaactcg ttgttcgtcc cgtacgtgat gtcacaagag
     3901 taggcctgtt gccgttccct ggcacccatg tcgtgttgaa tggcccccac cgttaggccc
     3961 aagaacatgt acagtgggcc catccactcc atgtctcgcc gggccaaata gtcgttcacc
     4021 gtgacgacgt gcacgccttt gccctcgagg gcattcagat aggcgggcag cgtggccacg
     4081 agggttttac cctcgccggt aaccatttcg gcaattgccc cggcgtggag gaccatcccc
     4141 cccatcaact gcacatcgaa atgccgcatc cctaggacac ggcgggaggc ctcccggcaa
     4201 acggcaaagg cttcaacgag gagatcatcc agcgtctcgc ccgcttccag tcgtttgcga
     4261 aacttcgccg tttgttcccg taattcagcg tcggtcatac gctgatagcg gggctccaga
     4321 tcattgatgg cctggacctt cggctgaagg cgtcgcagat agcgggcgtt ggccgagcca
     4381 aacagcgacg taatgccgcg gtccacggcc tgacccacca cgacgaaggt gtccgagacg
     4441 atttcccaaa ttctttccaa taattccatg gctctccaaa ggcccttgat attccagtca
     4501 agttttgttt gttttcttag ttacctcaag caccgatccg gtgacggctc cgcgtgcatg
     4561 ggctgtgtct taagaccaac atcagtgggg agatgcgagc ggtatcatga agacacaacc
     4621 aaccactgag gctgtagcaa aagacacggg ctaagcctct cataagagaa tccacgcggg
     4681 ccgcctgggg atgggcgacg aacaaatggc accaatttgc ttaatttaat cttatacgcc
     4741 ggcctctcga attcccgata cgactgcgca aattacgata tgctggcaat ttaccatctt
     4801 tgcgggggga ggtcaacaac aaaagggatt ggttttggga gctatttcag agcctatccc
     4861 ataatctccc cttgcccgtt cggcgggata ggtgtcgagg cgtgagagtg actcgaatcg
     4921 ctgccggttt tctcgatcga ctccgacgat cgcttgccct ctccccaacc ctctcccgga
     4981 gggagagggg gaacttttcg ggatagattt taagtgccaa tgaggcccga aaattttcct
     5041 cgatagggaa agcggtgctt gccccacggc cgagtcaaaa aaagataacc gcccctttgg
     5101 ggcggtcgtc ttgacactgc tatggcttgg tcagaggcca ggggaccggt ggctcaatct
     5161 aggtccctag ctcagcacgt cgctttgcaa gttgttgctg aagccgctgg acgatggcgc
     5221 tgtgcatctg actcacacgg ctttcggaaa ggtccagcgt ggcaccaatt tccttcatgg
     5281 tcaattcttc atagtagtac aggatgagaa tcaatcgctc gttgcgattc aggccttttg
     5341 tgattagccg catcaggtcg cttttttcga tgcgtcgcgt cgggtcttcg cccttcttgt
     5401 cttcgaggat gtcaatctcc ctgacatcct tatagctgtc ggtttcgtac ctcttcttgt
     5461 tgaggctgat caggttgatc gcgctggcct cggtcttcat cttttccagt tcttcgatgg
     5521 ttagccccaa ctcctgggcc agttcggagt cggtgggctg gcgtcctaac ctggcctcga
     5581 gcgtcttgca ggcctcgttc aacttgctgg ccttagaccg gactaggcgg gggacccaat
     5641 ccatcgtcct caattcgtcg agcatggccc cccgaattcg gggaacgcaa tatgtttcaa
     5701 acttgacccc cctggagaga tcaaaggcat cgatggcatc catcagtccg aagacgccag
     5761 cggaaataag gtcatcgagg tccacgcctt cgggaaggcg ggcccagatt cgttccgcat
     5821 tgtacttcac aagggggaga taaatctcca caagacggtt acgcaattcc tgattctgag
     5881 ggttcttctt gaattcgatc cagagctgct ccacatcctc gggagcgatt gttgtcggca
     5941 tagactcctc cctgatgctg cgatccttag aagaattctg cggttttgca atgggtgttt
     6001 tcgtctgcga tgccgttccc gtgaccgttc gaccggatga cgtagctctc gggcgactca
     6061 tccgtgtgtc tccctcttgc tggataaaag gtcctcacgg acgtgtattt tctcaatgct
     6121 cgcaccgtca ctgcggtgtt gtttcagatg ctagagcatc gatccgtaac ctatttattc
     6181 ctttcagcct gactctccga agccccgtta agtttttctg ccatttggct gcgtagtcgc
     6241 tgttcgacgg cctcacggag gacctgctcc gcgaccgctc cgatggccgc tccaacaaag
     6301 gaaaaaaata gcaggacgat ccatccccga agcaatgcgt cttgcggact aacaccgtgg
     6361 atcatgtccc ggattaatgc tgtgcacaaa cccaacgaac cgaacaccac ggcgtagata
     6421 cgtgccacgg gatcgcgtcc tgaacgtcac cgacggaatt cgtgtggatt gatttcaatt
     6481 acgctcggtc ccttgccgag ataatcaacc gaggctgttt tgaagcgtgg gcttaaggta
     6541 cccgcagtgc tccaataata tcggttgatt gttcggtaac gttgagtctg ttttcctgag
     6601 cttttgcatt tctccttatc gaaacgtgtt gcgacgttag taaactttgc caacgttact
     6661 tgttaggaat ttccgccatc ctttgcctga tctttttgcc aagggtttgc gtcggctttt
     6721 ctacgtcacg ccttctgtaa gtgctttcgc aatcagttgg atcgtttctt cccactcgcg
     6781 acgacagcag taggtgctcc catgttgggc gcttccgtcg gtcctcctca gccagttgcc
     6841 ttcgtcgaat tctccggcgt tcacgctttc gccttccgtt ctcggtaaga tcggcaaaat
     6901 aaacagactg gtcaggtctc gattcagaaa cctcccacag gcgcgttgga gtcggccggc
     6961 catttcttgc ccgatcgaaa gctcggccga cttgttgatc acgatcgtcg ggagaaatga
     7021 cgatgcaact cctgtggtcc gtttgaccag ggcgtaggcc ttcataatgt ctgtgggtgc
     7081 gggtgtggtc acgaggacac accgccgcga ggaatcgctg acaagctctt tgggatcatt
     7141 tgctacgcca caatctcgca agagtaagcg ttttcggctt tcgatgcccg ataggtgttc
     7201 tcttcctctg cgggttgtga aggccgtaga tatctcctgt tcccggtcac cgtgggacat
     7261 cgcggtttcc acccattgac cgtggtgctc aactcggaag ggagcgggtg gttttccaag
     7321 attctttggg acaccgttga aaaggcagac attccagccg acttgatgca aatagtgggc
     7381 gagggcctcg gcaaccgtgg aagtgccaac gccttgccga actccataaa tacggatctg
     7441 aagcgtgggt tgccgatcgt caaccgtcgc cgggcgaatc ctcgaaacca actgcctcag
     7501 gtgataggcc tggtcgtaca tgaccccaga ttgatccatg ctccgatatg attcactgaa
     7561 gttatcactc cccgctcctg cttgattgag gctgactcct taaggttggg ttatgaggga
     7621 tcccgaagaa cgttatccat tcccaaaatg agtcgggcta gtctgctggg ctgggcgatc
     7681 tcgatatcgt ctgggacatt ttgcccattg gtcagataac tcacaggcaa cccaccggac
     7741 cgcaacactg ggagcaaacc gccgaacgac ggcgcttcgt ccagtttggt gaggaccagg
     7801 gccgtcgggc cgaccgttcg gaagtgctcc agcgtctgca acaaagtctt agggctggca
     7861 acgctgctaa gtacgagatg aatttcgtct gcgtgcgcct cgttaaggag cgcacggagt
     7921 tcacgaatct tgatctcatc gcgaggactc cggccagccg tatcgatcag gataagatca
     7981 aatcccgtaa agtcctttac ggcatcgcgc atttcccgcg gtgtcgagac aacgtgcatc
     8041 ggcaagtcaa tgatatccgc gtaggtgcgc agttgttcca cagcggcgat tctgtacgtg
     8101 tccacagtga tcagagcgac gcgacggttc tcgcgaagcc gaaagttggc cgcgagtttg
     8161 gcaattgtcg tggtctttcc cacacccgtc ggaccgacga gcgcgcacac gcggcaacgg
     8221 ccgggcgtga tccggatggg ttgcgttgcg gccagttcgc tttccagaat acgcgccagg
     8281 cgggctttga cgagcatcgg ctcatccgtg cgatctcgga ccgactctgg ccgaacacgt
     8341 tccagaagtt ctcgcgcgtc ctcttctcga aggtcggcat cgatcaaatc ggtatagacc
     8401 ttgaacaggt gctcaggcca atcgtagggg acggaagaat tgttgcgacg gcataacgtt
     8461 cgcaacattt gctccatatt ctccaaccga tgctggactt caggatgaaa ggcccccgtc
     8521 cgcatgacca acgcgcgtgt ctccgcgctc gcgcgttcta gcgacaggcc ggtctccacg
     8581 accgactctc ggcattcccg gttcagtccg ccaagaggag aagaacttgg aggcagacga
     8641 cttggaacat tgacgttgac cgaggccgtg acttcgatct ccgcacgtcc tggcaaaagt
     8701 ccaaacaaac gacggacacg gatttcccgt gtgcgaagga tggcagcatc gggaccaagc
     8761 tctcttcgca caatggcaag ggcttcgtgc atcgtgctag cgcgaaacgt ccgaacgtcc
     8821 atagcatcca tgcgatttca tcctgacaaa tcgaaactcg ttggcatcta tgatttcagc
     8881 ttgtgagaca gctctttgct cctcaaggct cggttacgcg acatctgtta ccatggcgat
     8941 ggactcaatt cgcgtgtcgc gggtgatttc gttgtaacta agaacaacca aattcggtaa
     9001 atacggggtc atcatttctt tcagagctgg ccgaatctga ggactgacca aaacgaccgg
     9061 tggtcgatgc tgggtcgtca gcttgcttac ctcctgggca atgccgcggg cgattctctc
     9121 catcatttgc ggtgacatcc gaatgaacag gccgcgttcg gtatgatcaa atcctgcgcg
     9181 gatgcgatcc tccaaggcgg ggtcaagcgt gacaacaagc atgcggcctt ccgcatcgcg
     9241 atatcgcgtg gagatcgtcc gcgccagcct tgcccgcacg aattcggtca agaggatggg
     9301 gtctttagtg cgcggggcgt aatcccctaa cgtttccaga attagcccca actgccggat
     9361 gggcacctgc tcgcggagca acatttggag gatttgttgg acctctgcga gtcgcatcac
     9421 gttggggatc aagtcctcaa cgacggccgg agagctcttc ttcagttcct cgatcaggtg
     9481 tttggtagca tcgcgggtga ggatttcgtc cgcatgacgg cggatggttt ccgtgaggtg
     9541 agtagcaatc acggccgctg gttcaacgac ggtgtatcca tacatttcgg cccgttcgcg
     9601 ttgcgaaggc tcgatccaaa tcgcaggggc cccaaaggcg ggatctttgg tttcaattcc
     9661 cgggaccttt cccgtggtca tccccgaatc catcgccatc agcaagtggg cgtaaaccat
     9721 gccggttgcc acgggagcac cagcgatctt gattgtgtac tggtgttgat cgagctgaaa
     9781 attgtcacga atgcggacct taggcaaaat gatcccgatg tctccggcga tgtttttacg
     9841 aatgcgatgg atgcgatcga gcagatcacc accccgcttc ggatcggcca agcgaatcaa
     9901 gcccaccccg atctctagtt ccataggatc aacgagcaag tagtcctcga tccgttcctc
     9961 cgggggcttg gttgcctcgg cctgttttcg ggcttcttcc gattgcttct gtttccttgt
    10021 ttttcgtgat aaggtaacgg ccaaggcagc acaggcgcca ccaacggtta atagcgggat
    10081 ggtaggtaac tgtgtgaaga tcaaaatccc caagaaagcg ccggccactg ccaaggtccg
    10141 cggcctgcca aacatctgcc ggaggaactc tgccggaaga ttcacctccg tgctgctgcg
    10201 ggtaactaac agaccggccg ccaaggagat taagaacgcg ggaacctggc tgacaagccc
    10261 gtcgccgatg gtgagcttcg taaagagggc agcagcctga gagaggggaa ggcccatctg
    10321 gaccatgccg ataaacagcc cgccaatgat gttaatcaac gtgatgatga tgccggcgat
    10381 ggcgtcgccg cgtacgaact tactggcacc gtccattgcc ccaaaaaagt ctgcttggcg
    10441 ggtgatttcc tcacggcgtc tttgcgcctc ccgctcgtcg ataatccccg cgttgagatc
    10501 ggcatcgatc gccatttgac ggcctggcat cccgtccaac gtgaatcggg cggcgacctc
    10561 gctaatgcgg gttgccccct tcgtgatgac cacaaactga atcacgacaa tgatgataaa
    10621 gataatcaat ccaacaacga tttggtcacc tgccacaaat tccccaaatg cgcgaataac
    10681 actccctgcc gcctccagcc cggcctcgcc ggcgcgggtc aggatgagcc gcgtcgtggc
    10741 gacattcagc acaagccgca tgagtgtggt ggccagtagt agcgatggga agatactaaa
    10801 ttccaagggg gtggcgacat aaatcgtcgt caacaacatg ataatggcga tcgtaatatt
    10861 ggctgcgagg agaaaatcga gcagccagac gggcagtggg atcaagagga cgatgagact
    10921 ggcgatgatg gccgccggaa agataagctc ttgcggctta aagattaatc gcccaatgcc
    10981 tttggcgcct acatcacccg ctgacatgca agtccctaag cggtaccctg aatgctatca
    11041 agatgctccg aaacagcagc tctgtttgtg gttaagacgt tacccgaaac acggtcattt
    11101 tgtccaggtg gttttctaga cgggcgtacg tgcattggca gattattaga ttgtcaatgc
    11161 acgagagaaa agaaatgaat gtaggtcagg gttgcgtggt aagtgccatg ttgatag
//
LOCUS       DAOSUT010000133        11169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3288_length_11169_cov_9.686701, whole genome shotgun sequence.
ACCESSION   DAOSUT010000133 DAOSUT010000000
VERSION     DAOSUT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_11169_cov_9.686701"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLD05_11765"
     CDS             <1..667
                     /locus_tag="PLD05_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78161.1"
                     /translation="GGIRVPFLMQWKGKLPMGMVYHEMVMAFDIHATALAAAGIPLPT
                     DKPLDGVNLLPFLTGKQSGRPHEQLFWRAGRKYAARVADWKLVQESVGGPQLFNLKDD
                     LGEQHDLAQKYPDKLKELQAAFAEWEKGTQPAKWVRQDSRNAEIGGKLKAGGASAPAR
                     PGGGLESRFKQYDKNGDGKLTPAEYPLSFLAEADKNNDGALTLDEVKAYYVGRRQKRT
                     SEK"
     gene            1406..3922
                     /locus_tag="PLD05_11770"
     CDS             1406..3922
                     /locus_tag="PLD05_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78162.1"
                     /translation="MAVDSREGIAAASADGFVYLFSLDGKLRWKHDLGAPVLQVAFAK
                     LDGKSLVVLATGVSRELIALSTSGERLAAAKLNGAGRMMRAGDFDADGADEVVVLPIR
                     GQAMNAFFFKGPNLAKLVESIPSGTRMWDPEMGQRKKVGNAEPSAWNTLSLKKANGTV
                     ADLNGDGAMELIYSPGAYTLKNGLRQLVALPGPFKTASYDQYYNMRLVAAGNLTEEPG
                     AEIVVVEGPQVRLYDSTGKELGKAIAPFGFTDVVYSPGVPYGSVLLGSSPNGDDNIYR
                     LTFVPGWEKTLEALERRGVMAGIGTTLKQIADAVAGWQGEPMPGTDGPYDVVVNHSLW
                     SGWDPKKFDAWIAEVRAYEEAFPYSRLRFSYCIWPGEDAPLIRPDGKPWGRDHRLAHD
                     LTREQIVAAAKYFEAKGCYFWVQVGHGCDPHLEVATVAAMLEAAPKTLLGFVSAEDEQ
                     LDKVPYYFEHHVKPILELCLKHKKRFIPRNKDVWWAHWPADAKMRELIFNPRYRSVIV
                     PSVEDSNSRSPEVNLAARVGLWLDGQVDDWASRCCADWFCASRAWEWEYVMTGHPHLR
                     YYVSQAMLGARVFMMLNGERERVTGRWTRVGTEGTATFLHMLGKGVITPPRRDQLRAI
                     SPVALVMQQPSQRFAQHGANGHHEEYWGNDNTDSRPWAFDRLDTYWAMAPLPPTDVAT
                     YLWGRTRRDADHLPTTAPHGFVCIVPGGKPQTATRWSSLWMTDGDTLSKDGQNYSLLD
                     ARKAIIADLAHSAKTFPFHVEGEVFHQIIELNSDRYVIVLVDPGWLNPAERVVTLSAQ
                     RPGTWHVTDRLTGVSLGNLSQPLEVSVPAGALRLIELTLR"
     gene            complement(3933..4220)
                     /locus_tag="PLD05_11775"
     CDS             complement(3933..4220)
                     /locus_tag="PLD05_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78163.1"
                     /translation="MPILSAAETVITQQDLVSEDRGQQWGTVVIQAALPGTIIQLTQP
                     FQPVFELFARDVLSILADYGDCQEIRAKEIADVPAAIKHAPGPLLKQGLKS"
     gene            complement(4513..5607)
                     /locus_tag="PLD05_11780"
     CDS             complement(4513..5607)
                     /locus_tag="PLD05_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78164.1"
                     /translation="MNTARWGIVERLLKQVEGTMGRLVMAIAPNKPLDVVLYEAPVLV
                     SSQAPHNPLRGFVQEAVSLLEQPTAAEIADFLHLPTAVVELVLGNLQHLGGVTSDTAG
                     RWSVPEGAPRFQIGKQEPMVCRRTRKLLCYWVQREVLLPVLPRMRLRDMVALGVHKLQ
                     GEVADWYSQIASWDDVEGSKRGRPRTVRVLPLRSRCVLSGNAASASDTLEDASVTEDE
                     VLVSRCRLDVVALTWASFRSGIWEIASRLWSRPTSGKDGEDMPFAPGEPFLGLSLLEQ
                     LLGDSSSLDELGYLFSPHPEAWRKLLKNRDESRELRRDLDGNAPALIVGNQEQGDQHR
                     RWRGLFTSFAEEARLLCFLAPPAVGQSDPQ"
     gene            complement(5611..9306)
                     /locus_tag="PLD05_11785"
     CDS             complement(5611..9306)
                     /locus_tag="PLD05_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOP78165.1"
                     /translation="MSQTLVAKLRDLPLSQYILRGKSAIAAAQYEELRRLQEEQAAVT
                     PLESGPFRLFLSDSQESMLQEIGKDRRSLLASLNQGVDVVRVVPAKIGGGGLVIGFID
                     RGRLRIHLRHESEPPAPGDWRLLGVEVEVGMPEGPPLARLTARWSLFNRQDSDAVSGD
                     ADSVLKELRCTKKAYRAWIRSWKPPEIDAGSLVLHVSKKLQQHEASSDELLNDLFTGA
                     AKVFPVKRLSSEGRTRLLLFVPSHKVCLQLLPPDWPDAKAGHWVLHSLNRGVEKNDAV
                     VLLRVHWLSARQPYKTEGLSDDWLTQLAQAEQDYDDWKRLGWQPRHLNGGSFRLRITR
                     QKKEELEQSGLAPEKLCAAMSHGLDLYEVMGRECNVLVGVPEGLSCYIRLAPYKPSRR
                     DSDWRLVRIYRADSALPAFARIVARWEEGVSGVNPLSGDAPALIRDLEVLQRRRGEVH
                     QEITTLVECLTQNSPELSERIDCWKRVCQIERDMMEVGVASLERDGQDFSLVPEEQEM
                     VEEWIAGLDTLNPEGIVWDRYPLKLQVGDGHARLVIRSVKDATPENGEIALKVSCEDP
                     RGRALVEQLCDNAVAGEVEEREEVRLFLPDTQLGLIEQALNLLDPRQKTRSENRGIVG
                     EELAPDDLSLNTLRRILAAPDELEPMTAEWPPLPIPPLTPLSPKQEEAVRAAIFGPDM
                     TLIQGPPGTGKTTVILEILRQLFHLHGKDTGFKVLLVAPTHVAVDNVLERLIATRNAT
                     NLVMELGIAPYRVGSTRRIPEHLRGLTPDCLNTKYAEDLERDVARAAIQAEQEWHRDL
                     QVLEVLRDGARWDAESWALALKAGTLPESKSWEWPASLDEQWLTVLTTQEGRVRAWRH
                     WRARGTRPKERATLLQRWLDFLRNNPRFFSELLLANANLVCATTIGCATHPELRSIVY
                     DYVIVDEAGKEEARRLLVPLIRGERWILVGDHQQLPPYADDRLQAQLVRQGLDPKTVT
                     RSLFEELQGPFEERGRYVFLDLQGRMHPDISAFVSKQFYGGRLHDFPHVAAHELMCPA
                     FLPDAPKLLVLDTRHLPDRQETKRGTGYVNLLEQELVVLILQAFARLAIFQNSLADDV
                     GAGLPTIGVIAPYRRQVEELEHRVQRDPLLKALVRKKALHVGTVDSFQGLEKDLIIFS
                     CTRSNPQGRLGFVDNRQRLNVAFSRARCRLIVLADGCTVERARLRTDVSGIEAETRDH
                     LHALFAFSSDRGGVLEVPQDWRTRWSI"
     gene            complement(9317..>11169)
                     /locus_tag="PLD05_11790"
     CDS             complement(9317..>11169)
                     /locus_tag="PLD05_11790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOP78166.1"
                     /translation="MVGGFWYVRRYLQREAAYEDLRAPGRPRLPWFFNWFLRFPCSAV
                     VIFAFLTGLAVGAWGWGGWGPAIRSSLMQVIAPLPPLVTAIHSVVGEEAPPWPRWLDF
                     TLSLVYAWVVFALVTAWFERGRQRQNYVARLFTEEDRGGTPYGFRSGMSSDDLQPEPH
                     EQVILMQADRIGAACLPYLRVELSGPEWTTGPVAFDRCRGAVAAAGRVFTFAGDSCWR
                     SQPIESDWIADWLLTHLERLVDHDRSSPDPTFDHRLGEAVRAVAAVVASGQQLPPRPA
                     STSRGAYHDPERAKRLRTHLRELFLRSPKRPALLIALCEAAESLGTREDLQLLDEQSR
                     HLDVQAGFSRHQTDQILQTRDRVLGREPLRSELLKKLLQRIESLGLERLPVPEGRPLL
                     FRRPTDGAEMVLITAGSFWRGDDHTEETSPKRRVHLSSYLIDVVPVSQESFNLWAETQ
                     GRILRVDRGFFPVQGLPDDVPRDRPYASHVTWFAAQAYAQWAVVGGHLPTEAQWEKAA
                     RGSEDDRRYPSGEVWNEEPVSPYGVRVCHLLEWTQDAFDPEAYRHNPVVFDPRMESAA
                     GAGDEALRVVRGRAPESSVASYNLATRLGMEPITGAFTAPVGFRVAVDLE"
BASE COUNT         2330 a         3327 c         3238 g         2274 t
ORIGIN      
        1 agggggcatc cgcgtgccgt tccttatgca gtggaagggt aaactcccga tgggcatggt
       61 ctatcacgaa atggtcatgg ctttcgacat tcacgccacg gcattggcgg ctgccggtat
      121 tccattgccg accgacaaac cactggacgg cgtgaacctt ctccctttcc ttactggaaa
      181 gcagtcgggc cgcccacacg agcaactctt ttggcgggcc gggcggaaat atgctgctcg
      241 cgtggccgac tggaagctcg tgcaagagtc tgtcggcggg ccacaactct tcaatcttaa
      301 agacgacctt ggcgagcagc acgaccttgc gcagaagtat cccgacaaac ttaaggaact
      361 gcaagccgct ttcgccgaat gggaaaaagg cacacaaccg gccaaatggg tccggcaaga
      421 ttcccgcaac gcagaaattg gcggcaaatt gaaggcgggt ggtgcctctg cccctgctcg
      481 tcctggcggc ggtctcgaaa gtcggttcaa gcaatacgac aaaaacggcg acggcaaact
      541 gacgccagcc gagtatcctc tatcttttct tgcggaagct gacaagaata acgacggtgc
      601 cttgacgctg gacgaagtga aagcctacta cgtcggccgc cgacaaaaac gaacgagcga
      661 gaaatgagaa actaatcttc gaacagtttc ggcgcccctt tccgttgtca tttcggcgct
      721 ccgtcgaggt cctggggagg acaccggcgg agccgcgtgg ctacatgatt caccaagacg
      781 aaccggtcgg agcgaggctt cacacccggc cttgcggatg atgcgggcaa atcgggtccg
      841 catggtggtc cggatcccgc tttcaggatg actggcggtt tggcgataga cgccgaggga
      901 aacgacgtat tccgcccctg gctccgcttc ctcctctcga gtcagagacg tcggcggaaa
      961 ccgaaaaatc gtaccaatta agacgtgtcc ccggagtgag aacgaccgat ggagcaggac
     1021 ccgggtacgc tacaacgacc tcacctctcg tttcctagtt tgccccggtg acttgacgcg
     1081 ttccagggtg ccgagtaata gtgcttgctg gttccacacc ggcgcaactc ggtgctgaaa
     1141 tatgtacgtg acagcctggc aaataaaggc caaatgagag tattgtgcca tgcctttcag
     1201 cagacaattt ttgtgttgcc tattgattgg tgcaaacgcc gcgttcgccg acgtcgagat
     1261 tattccaacc ggagggcgga atatctaccg actggcaact caaccggggc tgattgtcgg
     1321 ctcgacgtac gacaatcgcg tttgcgcgtt caccgttacc ggaaagcact tgtgggatgt
     1381 gcccacaggc ggatttgtgt tcgacttggc cgttgacagt cgcgaaggga ttgcagcagc
     1441 cagtgccgat ggtttcgttt acttatttag cttggatggt aagctccgct ggaaacacga
     1501 tctgggcgcg ccggtcctgc aagtagcctt tgcaaagctc gacggcaaat cactggtcgt
     1561 gcttgccacc ggcgtgtcgc gcgagctgat cgcgctctct accagcggtg agcgcttggc
     1621 ggctgcgaag ctaaacggcg cgggtcgcat gatgcgggcg ggcgatttcg acgccgacgg
     1681 ggcggacgag gtggtcgtat tgcccattcg tggccaagca atgaatgcat ttttcttcaa
     1741 agggccaaac ctcgccaaac tcgtggaatc catcccgagc ggcacgcgaa tgtgggaccc
     1801 ggaaatgggc caaagaaaga aagtgggtaa tgcggaacca tcggcgtgga ataccttgtc
     1861 gttgaaaaag gcgaacggca ccgtcgccga cctcaacggc gatggcgcga tggaactgat
     1921 ttattcaccg ggcgcctaca cacttaagaa tggcctgcga cagttggtag ccttgccggg
     1981 gccgttcaag acagccagtt acgatcagta ttacaacatg cgtcttgtgg ccgctggcaa
     2041 cctcactgag gagcctggcg cggaaattgt ggtcgtcgaa ggcccccagg tgcgtctcta
     2101 cgattccacc ggcaaggagc ttggaaaagc gatcgcaccg ttcgggttca ccgatgttgt
     2161 ctattcgccg ggtgtgccgt acggcagtgt cctgctcggc tctagcccga atggcgacga
     2221 taatatctac cgactgacct ttgtgccagg ctgggaaaaa acgttggagg cacttgagcg
     2281 acgcggtgtc atggccggca tcggcacgac acttaagcaa attgccgatg cggttgccgg
     2341 atggcagggc gagcccatgc cggggaccga tgggccgtac gatgttgtcg tgaatcactc
     2401 tctctggagt ggctgggacc ccaagaagtt cgatgcatgg attgctgagg tccgcgccta
     2461 tgaagaggct tttccgtatt cgcgtttgcg gttctcctac tgtatctggc ccggcgaaga
     2521 tgcaccgtta atacgcccgg acgggaagcc atggggacgt gaccaccggt tggcccacga
     2581 cctgacgcgt gagcaaatcg ttgccgctgc aaaatacttt gaggcaaaag gctgctattt
     2641 ctgggtacag gtcggccacg gctgcgatcc acacctggaa gtggccacgg tcgcggcgat
     2701 gttggaggcc gccccgaaaa cgctgctggg gttcgtcagt gccgaagatg agcagctgga
     2761 taaagtaccg tactattttg agcatcacgt caagccgatt ttggaattgt gcctgaaaca
     2821 caagaaacgc ttcatcccgc gcaacaaaga cgtctggtgg gcgcactggc cggcagacgc
     2881 caagatgcgt gagttgattt tcaaccctcg gtatcgctct gtgattgtgc cgtcagttga
     2941 agacagcaat tcgcgcagtc ccgaagtgaa cctcgcggcg cgtgttggct tgtggcttga
     3001 tggccaagtg gatgactggg cgagccgttg ttgtgccgac tggttctgcg ctagccgcgc
     3061 gtgggaatgg gaatacgtaa tgactggcca tccacacctc cgctactacg tttcgcaggc
     3121 gatgcttggt gcgcgtgtat ttatgatgct caacggggaa cgcgaacggg tgacggggcg
     3181 ctggacgcgt gtcggcacgg aaggaacggc gacgttcctg cacatgctcg gcaaaggcgt
     3241 gatcacaccg ccgcgccgcg accagttgcg cgccatctcg cccgtggcgc tcgtcatgca
     3301 gcagcccagc cagcggttcg cccagcacgg tgccaacggt caccacgagg aatattgggg
     3361 gaatgacaac accgacagca gaccatgggc gttcgaccgg ctcgacacgt actgggcaat
     3421 ggccccgctc ccgccgacgg atgtggcgac gtatctttgg ggccggaccc gccgcgatgc
     3481 cgaccatttg ccgacgaccg cgccgcacgg tttcgtctgc atcgttcccg gcggcaaacc
     3541 gcaaaccgct acccgctgga gttccctttg gatgaccgat ggcgacacgc tgagcaagga
     3601 cggccagaat tattccctcc tcgacgcgcg gaaggccatc atcgcggact tggcccacag
     3661 cgccaaaaca tttccgttcc acgtcgaggg agaagtcttt caccaaatta tcgagctgaa
     3721 ttctgatcgt tacgtaatcg tgttggttga ccctggctgg ttgaatccgg cggagcgagt
     3781 cgtgacacta agcgcacaac gacctggcac gtggcacgtt accgaccgcc ttactggtgt
     3841 gtcgctgggc aacttgagcc agccgttgga ggtgtctgtc cccgccggcg cgttgcgttt
     3901 gatcgagttg acgctacgat gaccgacagg tactaggatt taagtccctg cttgagaaga
     3961 gggccagggg catgctttat ggcggcaggc acgtcggcaa tctctttcgc tcggatttct
     4021 tgacagtcgc catagtcggc caggattgag aggacatccc tcgcgaacag ttcaaagact
     4081 ggttgaaatg gttgtgtgag ctggattatt gtgcctggca acgccgcctg gataacaact
     4141 gtgccccact gctggccgcg atcttcgctt accaagtctt gttgcgttat aaccgtctct
     4201 gcagcagaga gaataggcaa gtctgctagg ccctcgacgt gctatgagtt cctgagcacc
     4261 ctcaactaag atccaaggct ctagctacgt aaaaacggtg tgtctcgaga atccatccgc
     4321 cggaaattgg acagcgtgtt cttcgtgagt atggcgacca caagtcgctt gacgttggtt
     4381 tcctcaggtg ccccttctca gggtgaaaga tgttcatgcc gcgggtgtta atgcgcagcc
     4441 gtatgtccaa cggctttttg atggacttga ggccttcaca tctccctcca aggacaatga
     4501 ctgtgctagt gactattgcg gatctgactg tcccacagcc ggaggagcca gaaagcaaag
     4561 gaggcgggcc tcttccgcga aggaggtgaa cagtccgcgc caacgacgat gctggtcgcc
     4621 ctgctcttga tttccaacaa ttagcgcggg agcatttccg tcaaggtcgc gacgaagttc
     4681 tcttgattcg tcccgattct tcagtagctt ccgccacgct tcggggtgtg ggctaaacag
     4741 atatccgagc tcgtccagac tcgagctgtc ccccagaagt tgttcgagta acgacaatcc
     4801 gaggaacggt tcacctggcg caaacggcat atcctcgcca tctttgcctg aagtcggacg
     4861 ggaccaaagg cgggaagcta tttcccagat acccgagcgg aaggatgccc acgtcagggc
     4921 gaccacgtcc aggcggcaac gactgaccaa tacctcgtct tctgttaccg aggcgtcctc
     4981 caaagtgtca gaagcactgg cggcgtttcc cgacagaacg cagcgactgc gcagaggcaa
     5041 gacgcgtacc gttcttgggc gccctcgctt agaaccttcg acgtcgtccc acgatgcgat
     5101 ctgagaatac caatccgcga cttcgccttg caatttatgc acgcccagcg ccaccatatc
     5161 gcgcagccgc atccgtggca agactggcag aagcacttcg cgctgtaccc agtaacacaa
     5221 gagcttgcgc gtccgacgac ataccatcgg ttcctgtttc cctatctgga atcgcggagc
     5281 gccttccggc accgaccatc gcccagccgt gtcgctggtc acaccgccca ggtgctgaag
     5341 gttacccaga acgagttcaa ccactgccgt gggcaggtgc agaaagtcgg cgatttcggc
     5401 ggccgtgggc tgttcgagaa gagaaaccgc ttcctggacg aacccccgca gggggttgtg
     5461 cggggcttga ctactgacca ggacgggggc ttcgtagagg acgacatcca gcggtttgtt
     5521 gggggcgata gccatgacca gacggcccat ggttccctca acctgtttga gtagccgctc
     5581 tacgatcccc caccttgctg tgttcatatg tcaaatgctc caacgcgttc tccaatcctg
     5641 tggcacctcc agtacaccgc cacggtccga ggaaaaggcg aacagagcgt gcaggtgatc
     5701 gcgtgtctcg gcctcgatgc cggaaacatc agtgcgtagc cgggcccgct ctactgtgca
     5761 accatccgca aggacgatga gacggcacct cgcccgcgaa aacgccacgt tcaacctctg
     5821 gcggttgtca acgaagccca gtcgcccttg ggggttgctt cgcgtgcagc tgaagatgat
     5881 cagatccttc tccagcccct gaaagctgtc aaccgttccg acatgaagcg ctttcttgcg
     5941 taccaaagcc ttgaggagcg gatctcgctg tacccggtgt tcgagctcct cgacttggcg
     6001 tcggtagggt gcgatcactc cgatggtggg aaggcccgct ccgacgtcgt cggctaggct
     6061 gttttggaaa atcgctagcc tggcaaaggc ttgaagaatg agcacgacta gttcttgttc
     6121 aagcaagtta acgtagcctg ttccacgctt ggtttcctga cggtcgggaa gatgtcgcgt
     6181 gtccaaaacg agcagtttcg gggcatccgg aaggaacgcc ggacacatca gctcgtgggc
     6241 ggccacgtgg gggaagtcgt gcaggcgacc accgtagaat tgtttagaga cgaaggcaga
     6301 aatatccggg tgcatccggc cctgaagatc gaggaagacg taacgtccac gctcctcaaa
     6361 tggaccctgt aactcctcga acaaggaacg ggtaaccgtc ttggggtcca atccctgacg
     6421 gaccagttgg gcctgcaagc ggtcatcggc gtagggcggc agttgctggt gatcgccgac
     6481 tagtatccat ctctcgccgc gaatcaaagg taccagcagg cggcgagctt cttccttgcc
     6541 ggcctcgtcc acgatgacat aatcgtatac gatggagcga agctcgggat gcgtggcgca
     6601 gccaatggtc gtcgcacaca ctagattggc gttggctaac aacaactcag agaagaagcg
     6661 gggattgttt cgcaagaaat ccagccaccg ttgaagcagt gtggcacgtt ccttgggtcg
     6721 ggtaccgcga gcccgccagt gacgccaggc acgaacgcgc ccttcctgag tagttagcac
     6781 ggtcagccat tgttcgtcga gcgacgcagg ccactcccac gatttgcttt ccggcagagt
     6841 cccagcctta agtgcaagcg cccacgattc ggcatcccac cttgcgccgt ctcgtagtac
     6901 ctccaacacc tggaggtcgc gatgccactc ctgttcggcc tggatagcag cccgggcaac
     6961 gtcccgttcc aggtcctccg cgtactttgt gttaagacag tcaggggtca agcctcgcag
     7021 gtgttcgggg atgcgacggg tggaaccgac gcggtacgga gcgatcccga gttccatcac
     7081 cagatttgtc gcgtttcttg tcgcgatcag ccgttctaat acgttgtcca cggccacgtg
     7141 ggtcggagcg accagaagca ccttgaaacc agtgtccttg ccgtggaggt gaaagagttg
     7201 tcggaggatt tcgaggatca ccgttgtctt gccggtaccc ggtggcccct ggatgagggt
     7261 catgtccggg ccgaagatgg ccgcccgtac cgcttcttcc tgtttcgggg acagcggggt
     7321 gagcggcggt atcgggagag ggggccattc cgccgtcatg ggttccaact cgtcaggagc
     7381 ggccaggatt ctccgaagcg tgttgagaga caaatcgtcc ggagccagtt cttcgcccac
     7441 gatcccgcga ttctcgctcc gtgtcttttg cctcggatcg agaaggttca aggcttgttc
     7501 gatcaacccc aactgtgtat ccggcagaaa caaccggacc tcttctcgtt cctcgacttc
     7561 gccagccact gcattatcac agagctgttc gactaacgcc cgcccgcgcg gatcctcgca
     7621 actgaccttc agggcgattt ctccgttctc aggcgtggcg tctttgaccg aacgaatcac
     7681 aagtcgggcg tgaccgtcgc caacctgtaa cttcagagga taacgatccc aaacaattcc
     7741 ttcggggttc agagtatcca gacctgcaat ccactcctcg accatttctt gttcttctgg
     7801 gactaatgaa aagtcctgac catcgcgttc cagggatgcc accccgactt ccatcatatc
     7861 gcgttcgatt tggcagaccc gcttccagca atcgattcgt tcagataact cgggcgaatt
     7921 ttgcgtgaga cactctacaa gcgtggttat ctcttggtgg acttccccgc gcctgcgttg
     7981 tagaacctcc aagtcgcgaa tcaacgccgg agcatctccc gaaagcgggt ttacccccga
     8041 cacaccttcc tcccatcgag caacaatccg agcaaaggct ggcaaggcag agtcggctct
     8101 gtagatccta acaagtctcc agtccgagtc gcgccttgag ggcttgtatg gtgccagccg
     8161 gatgtagcag gacagacctt ccgggacgcc gacgaggaca ttgcattcgc gccccatgac
     8221 ctcgtagaga tcaagaccgt gcgacatcgc agcgcagagt ttctctgggg cgagtccact
     8281 ttgctccaac tcctcctttt tttgacgagt gatgcgcaag cggaaagaac cgccgttcaa
     8341 gtgccgtggt tgccatccca gccgcttcca atcatcgtaa tcctgttcgg cctgggcgag
     8401 ttgagttaac caatcgtcac ttagcccctc cgttttgtag ggctgacgcg ccgagagcca
     8461 gtgcacgcgc agcaagacca ccgcgtcgtt tttttccacc cccctgttca gggagtgcaa
     8521 cacccagtgg cccgccttgg catccggcca gtctggagga aggagttgta ggcagacctt
     8581 gtgggatggc acgaaaagca ataacctcgt cctaccttct gacgagagtc gcttgacagg
     8641 gaagacctta gcagcaccgg taaacaggtc atttagtagc tcgtcagaag aggcttcgtg
     8701 ctgttgcagt ttcttcgaga catgtagcac aagcgatccc gcgtcgatct cagggggctt
     8761 ccagctcctg atccaagccc gatacgcttt cttcgtgcaa cggagctctt tgagcacgct
     8821 gtctgcatcc ccggaaacag cgtcggaatc ctgacggtta aataacgacc accgcgctgt
     8881 aagccgggcc aacggggggc cttccggcat tccgacttcg acttcgactc ccagcagacg
     8941 ccaatccccc ggagcaggcg gttccgattc atgtcgcaga tggatcctca gtcgccccct
     9001 gtcgatgaag ccaatcacca agccgccacc ccctatcttg gccggtacca cccggaccac
     9061 gtccacgccc tggttgaggc tggccaacaa gcttctgcgg tcctttccga tctcctgaag
     9121 catcgactct tgcgagtcgg acaagaagag gcggaagggg ccactctcca aaggcgtcac
     9181 cgcggcctgc tcctcctgca accgccgcaa ttcttcgtat tgagcggcag caatcgcgct
     9241 tttaccgcgc aagatgtact gggaaagtgg cagatccctc aacttagcca ccagcgtctg
     9301 gctcatgcct tacctcctac tcgaggtcaa cggcgacgcg gaaccctacg ggcgcggtga
     9361 acgccccggt aatgggttcc atccccagac gtgtggccag gttgtaactg gccacggaac
     9421 tctccggcgc gcgcccgcgg accacccgta gagcctcgtc gcccgcccct gccgcacttt
     9481 ccatacgggg atcgaatacg acggggttgt ggcggtaggc ctcagggtcg aatgcgtcct
     9541 gagtccactc aagcagatgg cacacgcgaa ctccgtaagg agaaaccggc tcctcgttcc
     9601 agacctctcc cgacggataa cgtctgtcat cctcacttcc tctcgccgct ttctcccatt
     9661 gcgcctccgt cggcaagtga cctcccacga ccgcccactg ggcatacgcc tgagccgcga
     9721 accatgtgac atgactggcg tatgggcggt cccgcggtac gtcgtcgggc agaccctgga
     9781 cggggaagaa gccgcgatcg acccgcagaa tacggccctg tgtttcagcc cagaggttga
     9841 aggactcctg ggatactggc acaacgtcga tgaggtaact gctcagatgc acccgacgct
     9901 tcggggaggt ttcttcggtg tggtcgtcgc cacgccaaaa agacccagcc gtgatcagga
     9961 ccatctcggc tccgtccgtg ggccgacgga acagcaaagg tcttccctcg gggacgggta
    10021 accgctctag cccaagcgat tcaatccgct gaagcaactt ctttaggagc tccgaccgta
    10081 acggttctcg ccccaagacg cggtcgcggg tttgcaggat ttggtcggtt tgatgtctgc
    10141 tgaacccggc ctggacatcg aggtggcggg attgctcgtc cagcaactgt aagtcttcac
    10201 gggttcccag agattcggcg gcctcacaaa gggcgatcaa cagggccggg cgcttgggcg
    10261 aacgtaaaaa gagctcccgc aggtgcgtgc gcaacctctt agccctttcg gggtcatggt
    10321 aagccccgcg cgaagtcgac gcaggccttg gaggcagctg ctggccgctt gccaccacgg
    10381 ccgcgaccgc cctcactgcc tctccaagtc gatggtcgaa ggtcgggtca gggctgctgc
    10441 ggtcgtgatc cactaggcgc tccaagtgcg tcagcagcca atcggctatc caatcacttt
    10501 ctatgggctg gctccgccag cacgaatcgc cagcgaaagt gaacacacga ccagccgccg
    10561 caaccgcacc ccggcagcga tcgaatgcga cgggccccgt tgtccattct ggccccgata
    10621 gctctacccg caggtagggg agacaggccg ccccgatccg gtctgcctgc atcagtatta
    10681 cctgttcgtg aggttcgggc tgtaaatcgt ccgacgacat ccctgaacgg aacccatatg
    10741 gtgtccctcc cctgtcctcc tccgtgaaca ggcgggcaac atagttctgc cgctggcggc
    10801 cccgctcgaa ccacgccgtg acgagggcaa aaacgaccca cgcataaacc agtgataatg
    10861 taaagtctag ccagcgcggc caaggcggag cttcttctcc gaccaccgag tgaatcgcgg
    10921 tcaccagagg aggtaaggga gcgattacct gcatcaggct gcttcgaatg gctggtcccc
    10981 agccgcccca tccccacgcg cctactgcta gaccggttag aaaagcaaaa atcaccacag
    11041 ctgagcatgg aaatcgaaga aaccaattaa agaaccacgg aagacgcggt cgccccggtg
    11101 ctcgcaagtc ttcgtatgct gcttcgcgtt gaagatatcg tcggacatac cagaagccgc
    11161 cgaccatca
//
LOCUS       DAOSUT010000134        11061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3326_length_11061_cov_10.042977, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000134 DAOSUT010000000
VERSION     DAOSUT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3326_length_11061_cov_10.042977"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1344
                     /gene="nadC"
                     /locus_tag="PLD05_11795"
     CDS             385..1344
                     /gene="nadC"
                     /locus_tag="PLD05_11795"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOP78167.1"
                     /translation="MKRDFLQVSWDQQIEDDVRRLVQLAIAEDWGERGDVTVQCTIPE
                     TATGKAAMVSRKRGILAGVLTVEVILAELSLDVQWKSLLTDGASLEKGTVIGRLQGRV
                     RDILSVERILLNFVGRLSGIATLTHSYVSAVRGTKARIYDTRKTTPGWRRLEKYAVRC
                     GGGHNHRLGLDRAILIKDNHLAWLAEELQCSLEDAAKIAIRRARELYPGHPIPSGQDP
                     ADIIVEIEVDSLPQLETVLCENPDIVLLDNMSLEELRQAVLLRNQLAPHVELEASGGV
                     TLENVRAVAETGVDRISVGALTHAAPWLDIGLDWGADGSGAVE"
     gene            complement(1446..1868)
                     /locus_tag="PLD05_11800"
     CDS             complement(1446..1868)
                     /locus_tag="PLD05_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78168.1"
                     /translation="MKSVQNASRIVISFLLAGAIFWATGCGSGGPPPKKDDSRNPASH
                     AQYIKNLTIRTLKKVPNLTKDEAVGLLQNMRAGLAQYEDVPMGEFEATYKEIVAGTEE
                     LLELCRQGGDKAQIKSRADALIEKANTLPGELEEETTG"
     gene            complement(1937..2971)
                     /locus_tag="PLD05_11805"
     CDS             complement(1937..2971)
                     /locus_tag="PLD05_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78169.1"
                     /translation="MKKAIVVRSTRGFTLVELLVVIAIIGILIALLLPAVQAAREAAR
                     RNQCTNNMKQVVLALHNYHDVNKQFPPAALGCTGAGFPYRALDTAAMAGKQPRNTLDT
                     WGATWITMLLPQLEQQALHSNYDFRRPCIDPVNQPVVKTQIATLICPSDASDNRVFIR
                     ATGQEFAKGNYVANCSARRYMSDLDGYTTLEYRGVFNTWVQWGAKIESILDGSSNTVA
                     ISEVCTSPETADARGAWGHVSGVAFGGGISGGSTPKGTLTPNANALDDNYRDLIEQYC
                     KAPNNDRRLRCATPGNAWDARIGARSYHPGGVNVGMADGSVRFIQETIDVTTWGALLS
                     SRGNEAVTLP"
     gene            3277..4449
                     /locus_tag="PLD05_11810"
     CDS             3277..4449
                     /locus_tag="PLD05_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOP78170.1"
                     /translation="MPRFEHDRLPRIALLIETSNEYGRRLLLGIRDYVREHEPWSFQL
                     GEQARGHTVPSWLSKWKGDGIIARIDNASIEQAVREVGVPVVNVSASGFGLAFPTVIS
                     DSEAIAQTAADHLLECGFSQFGYCGDPRFAWSKSHERHFKQYLQQRGFSCHCFSGSKS
                     AANHWGRALEELIGWVRELPKPIGIMACYDLRGQQLLATCREAGIHVPDEVAVIGQHN
                     DETICEFCDPPLSSVIPNPRLAGYLGASLLDRMLKGEWVPGGVYRVPPIGIAVRRSTD
                     VVAVPDEDVSAAIRYIRDHACQGIDVRDVVHAVNISRSMLERKFKRYLNCNPHDLIVR
                     TQLRRAQELLIQTDLTIENVATLSGFSSAEYMSCVFHRRVGQSPRDFRKRCRGKLL"
     gene            4664..6586
                     /locus_tag="PLD05_11815"
     CDS             4664..6586
                     /locus_tag="PLD05_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOP78171.1"
                     /translation="MTMRRLVAVWVGLFLAVVLPLVRQVPRALGESGGHGDGRVTISG
                     ELKRWHKVTLTLAGPFARERDESPNPFTDYDFTVEFRHESGEPVYRVPGYFAADGQAA
                     ETSAEAGNCWRAHLAPDKTGVWRYRVSFLKGKHAAIGGKGQPLAPYHGVSGQFTVAET
                     DKTGRDFRAKGRLAYVGKHHLQFLGTGEFFLKAGADAPETFLAYADFDGTIATKKNVP
                     LHKWEPHIRDWQPSDPSWKNGKGKGMIGALNYLASKGVNAFSFMPYNAGGDGDNVWPF
                     VERNDKFHYDCSKLDQWGIVFDHATSLGLYLHFKLQEQEMDDNRLGEKANKKVVPESL
                     DGGVLGPERKLYCRELIARFAHNLALNWNIGEENTQSAEEQRAMIQYIWEMDPYKHHI
                     VIHTFPNWQDRVYSELLGDKSLLTGASLQNGWREVHQRTLQWVQASAKAGRPWVVAND
                     EQGPADLGVPPDPGYQGFDGVARSGPEDTGYTLHDIRKYTLWGNLMAGGAGVEYYFGY
                     KLPQNDLTCEDYRSRDRSWDYCRIALEFFSRFNIPFWNMTNANALVGNEKNDNTRYCL
                     AAPGEIYVVYLPTGGTCELDLSKASGTFSVRWYDPRRGGDLQEGSVKEVSAGKKVGLG
                     RPPAEENEDWVILVRK"
     gene            6592..7671
                     /locus_tag="PLD05_11820"
     CDS             6592..7671
                     /locus_tag="PLD05_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOP78172.1"
                     /translation="METTFRIAVLPGDGIGPEVIREAVKVLRVIETHLQDAKFDLTEY
                     PCGAACFLATGEALPAETFEACQRADAILLGAMGMPSVRLPDGREITPQLDLREKLDL
                     FCGVRPIYLYHACDTPLKGYGPGEIDFVIVRENTEGLFSTRTANNSNGEVVSDTLRIT
                     RKGACRLFRAAFELAQTRRRHLTLVDKANVLPSMAFFRRLFLEVAHEYPDVTIDCCYV
                     DAMALYLLKSPQRFDVIVTENMFGDILSDLGAGLVGGMGMAPSGDIGERAAVFQPAHG
                     SAPDIAGRGIANPVAAILSTAMMLEWLKHPSTVRGAKWIREAVRRTLQQPELRTPDLG
                     GRLRTSEMGDLIIKEIEQLWCVSST"
     gene            7653..8282
                     /locus_tag="PLD05_11825"
     CDS             7653..8282
                     /locus_tag="PLD05_11825"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOP78173.1"
                     /translation="MRVLDLTLTISDGMPGVEVKPRHRRPQDAWNSSVLSLYSHCGTH
                     MDSPAHLNSSDSGIDLVPLEQCYGPAWVLRVPNVAPRSLILVEHLGSIAEQLQPGDGL
                     LIATGWSSRWGTPGYREALPRISRELALWCVNRQVRVLGVEPPSVADVNNRQEVEEIH
                     GILLKGGVIIVEGLTNLEAISKPRVIFAAFPLKIQGGDGSPVRAVAFEE"
     assembly_gap    8462..8561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8562..9182
                     /locus_tag="PLD05_11830"
     CDS             <8562..9182
                     /locus_tag="PLD05_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78174.1"
                     /translation="WLPTYLKQQWSNDPSAVYWSAVPLLCATAANWVAGGMVTRLHSL
                     GYPVASRRITAIVGFGLGTLGLLFAAQTESLFWFVVAFSITTFGVDMTLSPSWAFCND
                     LGGKHSGTISAAMNMVGNMGAAFGAIVFPILQDATTGRAETFFFMAASLNVIAIVCWL
                     FMNPMRRLNTSVSTRRLWGRFAVLMAILVILAVGLIGLQIYMTMQK"
     gene            9285..10346
                     /locus_tag="PLD05_11835"
     CDS             9285..10346
                     /locus_tag="PLD05_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78175.1"
                     /translation="MNAQDRRLKGVCVGAGYFSHYHYDAWQRIPEVEITAFVSRDPNR
                     IRAIQERFGIARVYSDYREALDREKPDFVDIITPPQTHLEICREVAARKIHIICQKPL
                     APTFEEAKTLVREVSQASVRFMVHENFRFQPWYRQIKKILDAGTLGRVFSLYIQTRMG
                     DGWGENAYLDRQPYFRHYPRLLIYETGVHFIDTFRYLLGEIRRVMAWLRRLNPVIAGE
                     DCGLMVCEFYSGAIGVWDANRYNEPNYENPRYTFGQTLLEGEHGSIRIYPDGRLTVQQ
                     LGGPEQPIAYEPSRHGFAGDCCYAAQRHFIDGLLTGQPFETNGWDYLKTLTVQEAVYQ
                     SATTGVPVEVTYGQEGDNV"
     gene            10343..10756
                     /locus_tag="PLD05_11840"
     CDS             10343..10756
                     /locus_tag="PLD05_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOP78176.1"
                     /translation="MSLFHRLDHIAILVADTDRALDVFHRKLGLPLVFSEVLEDQGVR
                     LTHLDMGGTQLQLVQPLRDDIPLAKVLAEKGEGLHHFCFYVEDLNEAVDSLKQNGIGC
                     RDAVPRSAPAGKRAVFLRPEDTYGVLIELTSHPVR"
     gene            10858..>11061
                     /locus_tag="PLD05_11845"
     CDS             10858..>11061
                     /locus_tag="PLD05_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOP78177.1"
                     /translation="MARIRETLSSRKSKVVVLDDDPTGTQTVYDIPVITEWSVASLRR
                     ELQAAGPGFYILTNSRSLTPAETE"
BASE COUNT         2444 a         2812 c         3168 g         2537 t
ORIGIN      
        1 gataccacgg tccatttgcc gtttgagcta cagcgtctga cggctggaga cgaccctccc
       61 gagccattgc ccgcaactgg tcgtcagaaa gcggaccaac gacttcggtt gagttggcga
      121 tccggcaata ccacatcgcg cgggccctcc cagtcgagag ctgcgagtta tccaccaatt
      181 caatacgacc gtcctaacat gccatcttct ccggtcttca tgagatagtt taacgcgccc
      241 ggcctattcc tgccaccaag cagaacttaa acggtgttgg catcataact tgacgggcac
      301 ggcacgttat tccctgccgt ggtatattcg aacttaagtg aaaataatcg cgttggtaag
      361 ctttcgcgaa aggagcatca ggtgatcaag cgggactttc ttcaagtgtc ctgggaccaa
      421 caaatcgaag acgacgtacg gcggctggtc caactcgcca tagcagagga ctggggggaa
      481 cgcggtgatg tgacggtcca atgcaccatt cccgagacgg ccaccggcaa agcggccatg
      541 gtgagtcgaa agagaggtat tctggcgggc gtgctcaccg ttgaagttat tctggccgaa
      601 ttgtcactcg acgtccaatg gaaaagcttg ttaaccgacg gggcgagtct cgaaaaagga
      661 acggtgatcg gccgattaca ggggcgcgtc cgcgacatcc tttcggtgga acgcatcctt
      721 ctcaactttg tgggacgact gtcggggatc gccacgctga cacattcata cgtctctgcg
      781 gttcggggga caaaagcacg gatctacgat acgcgaaaga cgaccccagg gtggcgaaga
      841 ctggaaaaat atgccgtgcg atgtggtggt gggcacaacc atcggttggg attggatcgc
      901 gcgatcttga ttaaggacaa ccatttggca tggttagctg aagagttgca atgttcccta
      961 gaagatgccg caaagatagc gatccgccgg gccagagagc tttaccccgg ccatccgatc
     1021 ccctcgggcc aggacccagc cgacatcatt gtggaaatcg aggtggattc cctgccgcaa
     1081 ctcgagactg tactctgcga gaaccccgac attgtgcttt tggataacat gtcgctggaa
     1141 gaactccgac aggcagtcct gttgcggaac cagcttgcac ctcatgtgga actggaggca
     1201 tccggtggcg ttacccttga aaacgttcgt gccgtggccg aaaccggtgt tgaccgcatc
     1261 agtgtggggg cattgaccca tgcggcgcct tggctagaca tcggtctcga ttggggggcg
     1321 gatgggtcag gcgccgtgga gtgaccacgg cctttcctgt cgcaaaagaa atggcccgac
     1381 gactgtcggg ccactcttca gattgggagg gttctaggga tttggggggc cgagttgttg
     1441 ggccctcagc ctgtagtctc ctcttccaat tctcctggga gcgtattggc cttttcaatc
     1501 agtgcatccg cccgcgactt gatttgagct ttgtcgccgc cttgtcggca caattcaaga
     1561 agttcctccg tgcccgcgac aatctcctta taagtcgcct cgaactctcc catgggaaca
     1621 tcttcgtatt gggcaagtcc cgcccgcatg ttttgcaaaa gacccacggc ttcgtctttc
     1681 gttaagttgg gtaccttttt aagggtacgg atcgtgagat tcttgatgta ctgagcatgc
     1741 gaagctgggt tcctggagtc atccttcttg ggcggtggcc caccgctccc gcagcctgtt
     1801 gcccaaaaga tggctccggc caaaagaaag ctaatcacaa ttctgcttgc gttttgaacc
     1861 gacttcatac ttacctcacg cttttgctta atcgcaccaa atcgtgccaa actgtcacga
     1921 caaataacgc tccacctcaa ggcaacgtga ctgcttcatt gcctcgagag ctgaggagtg
     1981 ccccccaagt tgtcacgtcg atggtctctt gaataaaccg cacggatccg tcagccatgc
     2041 caacattgac acccccagga tggtaactac gggcaccaat tcgagcatcc cacgcgtttc
     2101 ccggagtcgc gcaacggagt cgccgatcgt tattcggcgc cttgcagtat tgttcgataa
     2161 gatctcggta attgtcgtcc aaggcgtttg cgtttggggt caacgtgccc ttgggcgtcg
     2221 aaccaccact gatgccaccg ccgaaggcaa cacctgagac atggccccac gcaccgcgtg
     2281 cgtccgcggt ttcaggagat gtacaaacct cggaaattgc tacggtgttg gaggaaccat
     2341 caaggatgga ttcaatcttt gccccccact ggacccaagt gttgaacaca ccgcgatatt
     2401 ccaacgtggt gtaaccatct agatctgaca tgtaacgacg tgcactacag ttcgcgacat
     2461 agttcccttt ggcaaactct tgacccgttg cacggataaa gacgcggtta tccgaggcgt
     2521 ctgacgggca tattaaggta gcgatttgcg tcttgacgac cggctgattg accggatcga
     2581 tgcatgggcg gcgaaaatca taattggagt gaagcgcttg ttgttccaat tgtggtaaca
     2641 acatcgtgat ccatgttgcg ccccaagtat cgagtgtatt ccgcggttgt ttgcccgcca
     2701 tagctgccgt atccaaggcc ctataaggga aacctgctcc cgtgcagccc agcgcggccg
     2761 gcgggaactg cttattaaca tcatggtagt tgtgcagcgc caggacaact tgtttcatgt
     2821 tattagtgca ttggtttcgc ctggctgcct cccgtgccgc ttggactgct gggaggagaa
     2881 gcgcgatcaa aataccgatg attgcaatga ccaccagaag ctccaccagt gtgaagccac
     2941 gagtcgaacg gacgacaatt gcctttttca tagcatcgcc tccaaaaaag acctaaaaca
     3001 aacacgacga cgaacccaaa gctgtgccag ctcacccacc gttaccattt aaacaggact
     3061 gcttgctgct gtccaggtaa aaatacgtat tttttcttag attatgcgtc atttcacgct
     3121 tgttttaacc atctgtcttc ggacaaactg gctggctaca atgggcaagc gttggtggcc
     3181 taagggcgac agaaaagctt tatgaaacga gaccgatgag cgcgcaacct caccatggca
     3241 aaaaagaaca gagctaagcg aagggagggg gcgagcgtgc cccgatttga acacgatcgg
     3301 ttgccgcgga ttgccctcct aatcgaaacg tcgaatgaat atggacgtcg cttgctttta
     3361 ggaattcggg attacgtgcg ggagcatgag ccatggtcat ttcaacttgg ggaacaggcc
     3421 cgaggacaca cggtgccgtc ttggctttcg aaatggaaag gcgatgggat cattgccagg
     3481 atcgataatg cctcgatcga acaagcagtg cgcgaagtgg gtgtcccggt tgttaatgta
     3541 agtgcttcgg gctttgggtt agcgtttcca acggtcatca gtgatagtga ggccattgcc
     3601 caaaccgcgg cggaccacct tttggagtgt ggtttttccc aattcgggta ttgtggcgat
     3661 ccccggttcg cttggtctaa gagccatgaa aggcacttca agcagtatct tcaacagcga
     3721 gggttttcgt gtcattgttt ttctggttcc aagtcagcgg ctaaccactg gggacgtgcc
     3781 ctggaagaac tcatcggttg ggtccgggaa ctgccaaagc caatcgggat catggcttgt
     3841 tatgatttgc gtggtcagca actgctagcg acgtgtcgtg aggccggaat ccacgtcccc
     3901 gatgaggtgg cagtcatcgg acagcacaat gatgagacaa tctgcgagtt ctgcgacccg
     3961 ccgctttcaa gcgtgatccc gaacccacgg ttggccggtt acttgggggc atcccttttg
     4021 gatcggatgc tcaaagggga atgggttccc ggaggggttt atcgagttcc accaatcggc
     4081 atagcggtga ggcgttccac ggacgtcgtg gccgtaccgg acgaagacgt atcggctgcc
     4141 attcggtaca tccgggacca cgcatgccaa ggaatagacg tccgcgatgt cgttcacgcg
     4201 gtgaatattt cgcgaagcat gttagaacga aagttcaagc ggtacctcaa ttgtaatccc
     4261 catgatctaa ttgttcgcac acaattgcgg cgtgcccagg agttgctgat tcaaacagac
     4321 ctgacgatag aaaacgtggc cacgctgagc ggtttctcgt cggctgagta tatgagttgt
     4381 gtctttcatc ggcgtgttgg ccagtcgccg cgcgactttc gcaagcggtg tcgaggcaag
     4441 ttgctctgac aaaagagggg tgcaaccgca cagccgccga gtgtgaaaaa tcgaagaaaa
     4501 tttgtgcttt ttcacatggt gacaaccggt tctgcggatt acactggttc ttaggtcatt
     4561 cggcacgaaa actaaccgtg cgatgtgatg tctttcggtg agtgcgagta caacggtgtg
     4621 ccggctttga taggctgagg actaagctta aggagcgagg atcatgacaa tgagaagact
     4681 ggtcgctgtt tgggttgggc ttttcctcgc agtagttctt ccgttggtaa ggcaggtccc
     4741 cagagcactt ggcgaaagcg gtggtcatgg ggacggtcgc gtgaccattt cgggagagtt
     4801 gaagcgttgg cacaaggtga cgctgacttt ggcagggcca tttgcccgtg aacgagatga
     4861 gagcccgaat ccgtttacag attacgactt tacggtggaa ttccgacatg agtccggcga
     4921 gcccgtgtac agagttcccg gttatttcgc ggcggatggt caagcggcgg aaacgtccgc
     4981 ggaagccggc aactgttggc gagcccatct cgccccggat aaaacaggtg tctggaggta
     5041 ccgggtatca tttctcaaag gcaaacacgc ggccatcggg ggaaaagggc aacctttggc
     5101 cccttatcac ggagtaagtg ggcaattcac ggttgcagag accgacaaga caggccgtga
     5161 ttttcgtgcc aaaggccgtc tggcatatgt gggaaagcat cacctgcaat tcctggggac
     5221 gggcgaattc tttttgaagg cgggcgcgga tgcccccgag acattcctgg catacgcgga
     5281 tttcgacgga accattgcca ccaagaaaaa tgtgcccctt cacaagtggg aaccccacat
     5341 ccgcgactgg caacccagtg atccctcgtg gaagaacggg aaaggtaaag ggatgattgg
     5401 agcactcaac tatttggcgt cgaagggtgt caatgccttt tcgttcatgc catacaatgc
     5461 gggaggcgat ggcgacaacg tttggccgtt tgtggaacgc aacgacaagt ttcactacga
     5521 ttgctcgaag ttggatcagt ggggaattgt tttcgaccac gcaacatcac tgggacttta
     5581 cctgcacttt aagctgcaag aacaagagat ggacgataat cggctcggtg agaaggctaa
     5641 caagaaggtt gtcccggaat cgctcgatgg cggggtattg ggacccgaac ggaagcttta
     5701 ttgccgcgaa ctgatcgcgc ggtttgccca caatctggcg ctgaactgga atatcggcga
     5761 ggaaaatacg cagtccgcgg aagaacaacg ggcgatgatc caatatattt gggagatgga
     5821 cccgtacaag caccacatcg tgattcatac cttccccaat tggcaggatc gtgtttattc
     5881 cgagctctta ggggataagt cgctccttac gggcgcatca ttgcaaaacg gttggcggga
     5941 ggtccatcag cggacgcttc aatgggttca ggcctcggcc aaagcagggc gtccgtgggt
     6001 agttgctaac gacgagcagg gaccggccga tctgggtgta ccacccgatc ccggctacca
     6061 gggttttgat ggcgtggcgc gttctggacc ggaggatacg ggttatacac tccatgacat
     6121 tcggaaatac acattgtggg gcaatctgat ggctgggggg gccggtgtgg agtactattt
     6181 cggctacaaa cttccccaga acgatttgac ctgcgaagat taccgaagtc gcgaccgctc
     6241 ttgggattat tgccgcattg cccttgagtt cttttcacgg tttaatatcc cgttctggaa
     6301 catgacaaat gccaacgcac tggtgggaaa tgagaaaaac gataacacgc gttattgctt
     6361 ggctgctccg ggcgaaatct acgtggtcta tttgcccacc ggcggaacct gtgagctgga
     6421 tctctccaag gcatcgggaa cgttttcggt gaggtggtac gatccgcgcc gcggtggtga
     6481 ccttcaggaa gggagtgtga aagaggtgtc ggcgggcaag aaggtcggac tggggcgtcc
     6541 gccggcagaa gaaaacgaag attgggtcat ccttgttcga aagtgacaag catggaaacc
     6601 acttttcgca tagccgtact accaggggat ggtattggcc cggaggttat tcgggaagcc
     6661 gttaaggtcc tccgagttat tgagacacac cttcaggacg cgaaatttga tttgacggag
     6721 tacccgtgcg gcgcggcttg cttcctggcc accggtgagg ccctgccagc ggaaacgttc
     6781 gaagcctgcc agcgagcgga cgcgatcctc ctgggggcca tggggatgcc ttctgtccgg
     6841 ctacctgatg gtcgagagat cacaccccag ctcgacttgc gggaaaagct cgatctgttc
     6901 tgcggggttc ggcccattta tttgtaccac gcctgtgaca cacccttgaa gggttacggc
     6961 cccggtgaaa tcgattttgt gattgtccgg gagaacacgg aaggtctctt ttcgacgcgc
     7021 acagcgaaca actccaatgg tgaggtcgtt tcggacaccc tgcgaattac tagaaagggg
     7081 gcatgccgat tgtttcgggc ggcctttgaa ctggctcaga cgcgacggcg tcacctcact
     7141 ttggtagaca aggcgaacgt cctcccgtct atggcgtttt tccgccgctt gttcctggaa
     7201 gtggctcacg agtacccgga cgtcacgatc gattgttgct acgtcgatgc gatggcgcta
     7261 taccttttga aatcgccgca acgttttgac gtgattgtca cggagaatat gttcggtgat
     7321 atcctgtctg accttggggc ggggcttgtc ggcggtatgg gaatggcgcc atcaggagac
     7381 attggagaac gcgcggcggt ctttcagccg gcgcacggtt cggcgcctga cattgccggc
     7441 cgtggcatcg ccaacccggt ggctgcgatt ctctccactg ccatgatgct ggaatggctg
     7501 aaacacccct caacggttcg tggcgcaaag tggatccgcg aggcggtccg gcgaacgctt
     7561 caacaaccgg agctaaggac tcccgatttg ggaggaagat tacgcacgag cgagatgggg
     7621 gatttgatca taaaagaaat cgagcaacta tggtgcgtgt cctcgactta actctgacca
     7681 tcagcgacgg gatgccggga gtcgaggtaa agccgcggca tcgacgcccc caggatgctt
     7741 ggaattcgtc cgtactcagc ttgtattccc attgtggcac tcacatggat agcccggccc
     7801 atctcaactc ttcggatagc ggcatcgact tggttcctct cgaacaatgt tacggtcctg
     7861 cttgggtgct ccgtgttccc aacgttgcac cgcgaagcct tatcctcgtg gagcacctcg
     7921 ggagcattgc ggagcagctt caacctgggg acggcttact catcgccaca ggctggagtt
     7981 ctcgttgggg aacgccaggg tatcgggagg cgttgccgcg gattagtcga gagttggcgc
     8041 tctggtgcgt taaccgccag gtccgagttt tgggagttga accgccgtct gtggccgatg
     8101 tgaacaatcg gcaagaggtc gaggaaattc acggcatcct cctaaaaggc ggagtgatca
     8161 ttgtggaagg attgaccaac ctggaagcaa tcagcaaacc gcgcgtcatc ttcgcagcgt
     8221 tcccattgaa aattcaaggg ggtgatggtt cgcctgtgcg tgcggtggcc tttgaagaat
     8281 gaaaaagcgg tggagcaagg tgttggccca tggacaatcc gttgcaacga tcacggattc
     8341 ggatttgggt gatcacgatc cttttctttt gtagtcttaa cacttacctc gatcgtgctt
     8401 gcattgcttc cgcggttgac gcaattcggc atgaccttca cttgtcagct caggctatgg
     8461 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggctgccg acttatctca
     8581 aacaacaatg gtccaatgat ccttctgcgg tctattggtc ggcggtccct ttgttgtgcg
     8641 caaccgcagc caattgggtg gcgggcggca tggtgactcg gctgcactcc ctcggttacc
     8701 cggtagcatc gcgtcgaatt acggcaattg ttggcttcgg gcttggaacc ttggggttgt
     8761 tatttgccgc gcagacagag agcttattct ggttcgttgt tgcgttcagc ataacgactt
     8821 ttggtgtgga catgacgctc agcccaagtt gggccttctg caacgactta ggggggaagc
     8881 actcgggaac gatctctgcc gcaatgaaca tggttggtaa tatgggtgct gcgttcgggg
     8941 ccatcgtttt tcccattctt caggatgcta ccacggggcg agcggaaacc ttcttcttca
     9001 tggcagcgag ccttaacgtg atcgctattg tctgctggtt gttcatgaat cccatgcggc
     9061 gtttgaacac gtcagtatcc acgcggcggc tttggggacg attcgccgtt ttgatggcaa
     9121 ttttagtcat ccttgcggtt ggcctgattg gcctccaaat ttatatgaca atgcaaaaat
     9181 aggatcgggc acggggccaa taggccaacg cagacagcag acgcctgatc catcaaggat
     9241 tgaaattggt gccgcgcggg aacgtccaaa ggagaagatc ggccatgaat gcccaagacc
     9301 gacgcctcaa gggtgtttgt gtgggagcag gctactttag ccattaccac tacgacgcct
     9361 ggcagagaat tcccgaagta gagatcaccg cctttgttag tcgtgatccc aatcgcattc
     9421 gggcaatcca agaacgcttt ggcattgccc gggtttattc ggattaccga gaggcactcg
     9481 accgggaaaa gccggatttt gtggatatca tcacgccgcc tcaaacccac ctggagatat
     9541 gtcgagaggt agccgcccgc aagattcata ttatctgcca aaagccactg gccccaacct
     9601 tcgaagaagc aaaaacgctg gtgcgcgagg taagtcaagc cagcgttcgc ttcatggttc
     9661 atgagaactt ccgctttcag ccgtggtacc ggcagatcaa gaagattttg gatgccggga
     9721 cattggggcg tgttttctcg ctttacattc aaacgcgcat gggcgatggc tggggcgaaa
     9781 atgcctatct tgatcgacag ccgtatttcc ggcactatcc tcgactgctc atttacgaga
     9841 cgggtgtgca ttttattgat acattccgct atttgctggg agagatacgt cgggtcatgg
     9901 cctggcttcg ccgcttgaac cccgtcatcg ctggagaaga ttgtggcctg atggtgtgcg
     9961 agttctattc cggcgcaatc ggcgtttggg acgcgaatcg ttataatgaa cccaactacg
    10021 aaaaccctcg ctacacattc gggcagacgt tgctggaagg cgaacacgga tcgattcgca
    10081 tatatccgga cggacgtttg actgtccaac aactaggagg ccctgaacag cccatcgcct
    10141 acgaaccatc gcgacatggt tttgcgggtg actgctgcta tgcggcgcag cggcatttta
    10201 tcgatggact gctcacaggt cagccattcg aaacaaacgg gtgggactac ctgaagacat
    10261 tgacggttca ggaggccgtt tatcagtcag cgaccactgg ggtcccggtt gaggtgacct
    10321 acggtcagga aggagacaat gtatgagctt gtttcaccgc ttagaccata tcgccattct
    10381 tgtcgccgat acagatcgcg ccctggatgt gtttcatcgc aagctggggt tgccgcttgt
    10441 ctttagcgaa gtcctggagg atcaaggagt gcgattgacg catctcgata tgggagggac
    10501 gcaactccag cttgttcagc cgctgcgtga tgacatcccc ttggccaaag ttttggccga
    10561 aaaaggtgag ggtcttcacc acttttgctt ctatgtggaa gatctgaacg aagcggttga
    10621 cagcttgaag caaaatggta tcggttgtcg cgacgccgtg ccgcgaagtg ctcccgccgg
    10681 aaagagggcc gtatttctca ggccggaaga tacgtatggt gtgctaatcg aattgaccag
    10741 tcatcctgtg cggtaactgg aaacgctgga atcaccatgg atagcacgga cttctgctcg
    10801 cttttcccaa gggaacgtat attgaaggcc ttgccacccg tatggccaga gtcgctgatg
    10861 gctcgaataa gggagacttt gtcttcccgc aaaagtaaag tcgttgtctt ggatgatgac
    10921 ccaacaggaa cgcaaacggt ttacgatata ccggtcatca cagagtggtc agtcgccagc
    10981 ttacgtcgcg agcttcaagc ggccggtccc ggtttctaca tcctcacaaa ttctcggagt
    11041 ttgactccgg ccgagacaga g
//
LOCUS       DAOSUT010000135        11008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3339_length_11008_cov_8.884233, whole genome shotgun sequence.
ACCESSION   DAOSUT010000135 DAOSUT010000000
VERSION     DAOSUT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_11008_cov_8.884233"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1553)
                     /locus_tag="PLD05_11850"
     CDS             complement(354..1553)
                     /locus_tag="PLD05_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOP78178.1"
                     /translation="MNRLVCFTGLLMILFVAWSLSENRRAMNWRLIISGLVMQWIIAL
                     IMLKTGVGQAAFSGGRILIERLQACVNQGTSFVFGPEAQHHQVAFVVLPLIIFVSSLS
                     AVLFYLGILQWLVKIMAKVMVWVMDVSGAESLAAAANIYVGMTEAPLIIRPYLSTMTR
                     SELMAMMSSGMATIAGTVMAAYVAMGVDAGHIMTASLMSAPASLVVAKIMVPELELSP
                     TKGIVRVAVPREDVNLLDAACRGASDGLMLTLNVAAMLIAFIALIAFVNWILGLFGNV
                     GNQPLTLERITGYLFVPFAWAMGIEWKDIPTIARLLGLRTVLNEFIAYQELTSIKETL
                     SPRSFIIASYAICGFANFGSIGVMIGGIGRLVPERRSELAILGFRSMVAGTLACYMTA
                     CIAGILL"
     gene            1963..4482
                     /locus_tag="PLD05_11855"
     CDS             1963..4482
                     /locus_tag="PLD05_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOP78179.1"
                     /translation="MLMTYWRSALRNMGMVLAMVMACVAMPTFIEAEDVKIMTVSTPD
                     AGLGIGHYPANQPPLMPSHLAKLRVGAVRAEGWLLTQLKLEADGFIGHLHELSRFLAD
                     EGNAWLNPAGEGDRSFWEELPYWLKGYGDLAYLLGDEKMIAEARRWIEATLASQREDG
                     YFGPRRNLEMVNTPREKKPDHWPNMVMLHALQSYHEFTGDPRILELMKRYFRWQLSQP
                     DEDFLLPFWQNQRASDNLVSVYWLYNRSPEPWLLELGAKIHRCMARWDTGVVNWHGVN
                     MAQCFRAPGIYYQQAGDRKLLQMAYANYEEMREKYGQVPGGLYGADENCRPGYTDPRQ
                     AAETCAMVEMMYSCELLTAISGDVIWADRCEDVAFNSLPASMTANLKALRYLTAPNLA
                     VSDGKNHAPGFQNAGPMLLFDPYGHRCCQHNVSHGWPYFVEHLWMAAPGDGLAAVLYG
                     PCRVRAKVGKSGNEVTISEETQYPFRDTITFTVRAATPTDFPIYFRIPGWCTNPQVEI
                     ASAGGAKITPEIPGEKAGWLVLWRMWQDGDRITLRLPMTVKLRRWERNHNSVSVDRGP
                     LTYSLKIGERYVQAGGTEKWPAWEIYPTTDWNYGLVLRDDDPAAGFEVVERPWDGVSQ
                     PFTPETAPVVLQTKGKQIPQWKLDQWGLVAPLQASPVRSSSPETTIELIPMGCARLRI
                     SAFPVIGTGPDAVEWKELSLPPHEASHCFEHDTVAALSDGLEPKSSFDRTIPRFTWWP
                     RRGTEEWVTYRFDTPRHVSEVQVYWFDDTGAGSCRVPASCRVEWWDGENWQPVRPDPL
                     CGVEKDRYNVLRFDPMTTTQLRLMVQLREGFSAGILEWRVK"
     gene            4716..5450
                     /locus_tag="PLD05_11860"
     CDS             4716..5450
                     /locus_tag="PLD05_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP78180.1"
                     /translation="MSTLQRSSRRCDNLISGFGLGVFLILGFTAVLFASGASAQNISF
                     QRVEIDAEPPKSPWLKLAGDFNGDGKLDIAIGGASMYLVWYVNPTWQKITIAEKGWQT
                     VSGAVGDIDGDGDVDILPGAQIWLENPLPKGDPTKGPWPVRRISNVRTHDALLADLDV
                     DGRLDIVARDQSGFNHNTGNKIHMWYQRDLDRWDYHVVDCPHGEGLAVADLDRDGDPD
                     VIIAGIWYENPGKAGGDWKSHQFTAQ"
     gene            5469..5900
                     /locus_tag="PLD05_11865"
     CDS             5469..5900
                     /locus_tag="PLD05_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP78181.1"
                     /translation="MAVGDINGDGRCDIILSPSEYKGQTYQVAWYEAPSDPASPEWAQ
                     HVIIPAIESVCHSLQVADMDGDGLLDIVTARMHQGAPPQEVMVLLNREKGRKWEKLVV
                     STRGSHDLLVADFDGDGRPDILGANHGGPYQPVELWLNRAK"
     gene            6164..6472
                     /locus_tag="PLD05_11870"
     CDS             6164..6472
                     /locus_tag="PLD05_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOP78182.1"
                     /translation="MVLLEFSMYPLDKGPSLSAYVAKSLEIIDKSGLDYQCHAMGTII
                     EGEFDQVMDVVKRCFQAMQAECDRIECIIKIDYRKGQSGRIRSKVQSVEAKLGRPVKK
                     "
     gene            complement(6515..7645)
                     /locus_tag="PLD05_11875"
     CDS             complement(6515..7645)
                     /locus_tag="PLD05_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOP78183.1"
                     /translation="MNLRPEELQTFKTALDFAQRQVEHLIEKHPDFFPIYTVQGKWYH
                     EGELWTDWTGGFLAGMMWRFYRYTSDEKWLERAIHYSKLLEHRKHDRNVHDLGFIFLN
                     TYLPWYELTKDPALQEVLITAGRTLAMRYQPKGRYLCSFIGPQSLFIDIMMNVPIVFY
                     AAIHTNDDRLMQIALDHCRTTRDRLVREDGSTAHEGLFDVETGEFLGQSTHQGLSAES
                     CWARGLAWSMYGFSKVYGLTQNPEFLEVAEKNAEYWITHLPEDKVPYWDFDADLSQPP
                     PWGRQKESSAAAIAASGLLDLAQQTASPERSVVYRNTALVMLMALSQPEYLAIETPGW
                     EGILKHGVYHTRKNLGVDESVMWGEYFYVEALTKLIPHQNAS"
     gene            7772..7963
                     /locus_tag="PLD05_11880"
     CDS             7772..7963
                     /locus_tag="PLD05_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78184.1"
                     /translation="MPEKSAHEKNLFTDAIAVELTGFLWGKVFIGCEYLMMFIHLKQN
                     FSFFVGMPPRDDSKGYVVD"
     gene            8463..10307
                     /locus_tag="PLD05_11885"
     CDS             8463..10307
                     /locus_tag="PLD05_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP78185.1"
                     /translation="MLCELTARVSVLVAILFGLLAADAAKAAEPADPDLIPEARRVLE
                     YLKSLQGKQMLSGISGSQDAQPWAVLHMTGREPAIAGGDMAGFHRKWEKQYHQVMQNT
                     VDKCIHWWRDKGGIVALQYHWMKPGEPEGSAWIGPPRGTGRLDLAKAVTPGTEEHRAV
                     MADLKVTADYLEQLAEARVPVLWRPLHEIDGGWFWWTDAETPENTAALWRLMFDYFVR
                     ERKLHNLIWVYNAAHVSHTRKPPAATFEEEVAHRRRYYPGGEYVDIASIDTYANPKLG
                     WSAAQEDARRRAYELMQQVAPGKMLAVAEDAALLNPDVFRREGPAWLYCSAWWTGGKE
                     NPVEWMRYTFNHEAYVTLDELPLLVEGNVMPNVRITDPSDGLALNGSNVVISGFASDR
                     NGNLRRVTVHALRGPWRNWFLRADADVLRAFPDSTQLGEASVAPDGQWTFTWQNAPSG
                     VYNLVALARDAEGAVTCSNVVRVTVGIHNLARGVKSVASSTSKWGGPPAAAIDGDPNS
                     MWWSDNDQPDPQWLMVDLGTEQKVGAVSVLWWKAYAKDYTVEVSTDGTSWREVARVEG
                     RSNWLGDMDVFRFEPTTARYVRLHCTSRAVTWQAYTVYELGVYESIPE"
     gene            complement(10826..>11008)
                     /locus_tag="PLD05_11890"
     CDS             complement(10826..>11008)
                     /locus_tag="PLD05_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78186.1"
                     /translation="TEGNGNILDSFLGKVSVNITRKESFLREFGSHAKINFPEKWHPK
                     NGKSRAQGDGKIGQLY"
BASE COUNT         2434 a         2877 c         3232 g         2465 t
ORIGIN      
        1 gggctcaaat aacagtgcag agcccggttc tccgtttctt cgattaggag cctataccgc
       61 aagctcgccc tcgcccgttc ggcgggacga ggccggcggt gagggtgacc cgaatcgctg
      121 ccgttttctc gatcgactcc gagggtcgct agccctcacc ccaaccctct cccagagtag
      181 gagggataat tggtgggctc taagccactg cttcctccca aatacagctc gtggcaacaa
      241 agcccctttg aatgtgaggt cacggactca ggacccaacc agttcacctt cgatattcca
      301 gactgaccga cgcaggcctc ctgtaccgat cgttgtcttg gcggggaaat ggcctataga
      361 agaattccgg cgatacaggc cgtcatgtag caggcgagtg tccccgccac catcgaccga
      421 aatccgagta tggccaattc cgatcgccgt tcgggaacga ggcgcccaat tcccccaatc
      481 atcacaccga tcgacccgaa gttggcaaag ccacagatgg cgtacgacgc aatgatgaat
      541 gagcgcggcg aaagtgtctc cttgatgctt gtcagctctt ggtaagcgat aaattcgttg
      601 agaacggtcc gcaggccgag gagcctcgcg atggttggga tgtctttcca ttcgattccc
      661 atggcccagg cgaagggcac aaatagataa cccgtgatcc gttccagcgt cagtggttgg
      721 tttcccacat tcccgaacag ccccaagatc caattgacaa aggcaatgag ggcaatgaac
      781 gcgatgagca tggccgccac gttcagtgtc agcatcagac catcgcttgc tcctcggcaa
      841 gcggcgtcaa gcaggttgac atcttccctg ggtacggcca cgcgaacgat cccttttgtc
      901 ggtgagagct caagctcggg gaccatgatc ttggcgacga cgagcgaggc cggtgccgac
      961 atcagtgagg ccgtcataat gtgtccagca tcaacgccca tcgccacgta agccgccatg
     1021 acggtccctg cgatggtggc catgccgctg ctcatcatgg ccattaattc ggaacgagtc
     1081 atggtgctaa ggtatggccg gatgataagg ggcgcttcgg tcatgccgac gtaaatattc
     1141 gccgctgcgg caagggattc tgcaccagag acatccatga cccagaccat caccttggcc
     1201 atgattttca ccaaccattg gaggattcca agataaaaca gcactgcgga gagggacgaa
     1261 acaaagataa ttagtggcaa gacgacaaag gctacttgat gatgctgggc ctcgggaccg
     1321 aagacaaagc tcgtgccctg gttgacgcac gcttggagcc gttcgatcag aatacggcca
     1381 ccggagaagg ctgcctgtcc gacccccgtt tttaacataa tgagggcaat gatccactgc
     1441 atgactagtc cactgatgat aagccgccag ttcatcgccc ggcgattttc ggaaagactc
     1501 cacgccacaa agaggatcat cagaagcccg gtgaaacaaa ccaatcgatt catcggtaag
     1561 cctccattta ctgacgcact ttatccatgt tcgtgagaat caagccgtgt gtccgttttc
     1621 tcaacccaga aaaccaccag tgggcaaaag ctcattgcat gagtcaatgt aagaccaatt
     1681 cgggaatatg cttccttcat tgccgcatgc tgagaacggt aatataagcg tcaggagcca
     1741 tctttcaaag gatgcgtggt tcaggcattg cgcggcttga caactgcccg cggagcgccc
     1801 aaattgaatc atcctcggcg aaaggctgtt aggctacctc agaaaacctt tggctttccg
     1861 gggaggccag ggttgtcctc aatgtaccca ttgagaaatg aagcaaccta aaggttctta
     1921 cttcggcgat tcggtcactc ttatttaacg ggggaatgac tcatgttaat gacctattgg
     1981 cgaagcgcgc taaggaacat ggggatggta ctggccatgg taatggcatg tgtggccatg
     2041 ccaacgttta tagaggcgga agacgtgaag atcatgactg tttccacgcc cgatgcgggc
     2101 ctcggtattg ggcattatcc ggccaaccag ccgccactta tgccaagcca tctagcaaaa
     2161 ctacgtgttg gggctgttcg cgcggaaggg tggcttctaa cgcaattgaa gctcgaagcc
     2221 gatggcttta tcggtcatct tcatgagttg agtcgttttc ttgcggacga gggtaatgcc
     2281 tggctcaacc ccgcagggga aggggatcga agcttctggg aggagttgcc ctattggctg
     2341 aagggttacg gcgacctggc ctatctgctg ggcgatgaga agatgattgc cgaggcccgc
     2401 cggtggattg aggccacact tgctagccaa cgggaagacg gttatttcgg tccgcggagg
     2461 aatcttgaga tggtcaacac tccgcgggag aaaaagcccg atcactggcc caacatggtc
     2521 atgcttcatg cgttgcaatc atatcatgag tttacaggtg atcctcgcat cctggaacta
     2581 atgaaacgct attttcgctg gcaattgtcg cagccggatg aagattttct gcttccgttt
     2641 tggcaaaacc agcgggcgag cgacaacctc gtcagcgtct attggcttta caatcgttcg
     2701 cccgaaccat ggctgctgga gttgggagcc aagattcatc gctgtatggc gcgatgggac
     2761 acgggcgtgg tgaattggca cggggtgaat atggctcaat gcttccgcgc accggggatc
     2821 tactatcagc aggcaggcga ccgcaaactc ctacaaatgg cttatgcgaa ttatgaggag
     2881 atgagagaga agtacggcca ggtcccgggt ggtctttatg gagcggacga gaactgtcgg
     2941 ccagggtata ccgatccgcg tcaggcggcc gagacctgtg ccatggtgga gatgatgtac
     3001 tcctgcgaat tgctgactgc gatttccggc gatgtcatct gggccgatcg gtgcgaagat
     3061 gtggccttta actcgctgcc ggcctcgatg acggccaacc ttaaggcttt gcggtacctg
     3121 accgctccta acttggcagt ctcggacgga aaaaaccatg caccgggatt ccagaacgct
     3181 ggaccaatgt tgctttttga cccttacggt caccgttgtt gccaacacaa tgtttcccac
     3241 ggatggccgt atttcgtcga gcatttatgg atggctgccc ccggtgatgg tttggcggcg
     3301 gtgttgtatg gtccttgccg tgttcgcgcg aaggttggaa aatccggcaa cgaagtgaca
     3361 atcagcgaag aaacgcaata cccgttccgg gacacgatca cctttaccgt gcgggcggcc
     3421 actccgacgg acttccccat ttacttccgt attccaggct ggtgcacaaa tcctcaagtg
     3481 gagattgcca gcgccggtgg ggccaagatt acgccagaaa taccgggtga gaaggccgga
     3541 tggttagtat tgtggcgaat gtggcaggac ggagatcgga ttacgttgcg gctaccgatg
     3601 acggtcaaac ttcgtcgttg ggaaaggaat cacaatagtg tctcggtgga ccggggaccg
     3661 ttgacatatt cgttaaagat tggtgagcgg tatgttcagg ccgggggaac cgaaaaatgg
     3721 ccggcctggg agatttaccc cacaaccgat tggaattacg ggctggtatt acgggacgat
     3781 gaccccgcgg cagggtttga ggtggtggag cgtccttggg atggtgtgtc gcagcccttt
     3841 acgcccgaga ctgcccccgt ggtgctgcag acaaagggaa agcaaatccc acagtggaaa
     3901 ctggatcaat ggggcctggt agctccgtta caagctagtc ccgtgcgttc atcgtctccc
     3961 gagacaacga tagaactgat tcccatgggt tgtgcccggt tacggatcag cgccttcccg
     4021 gtgatcggca caggccccga tgccgtcgaa tggaaggaac tgagcttgcc tccgcatgag
     4081 gcctcacatt gttttgaaca tgacacggtg gcggccctca gcgatggttt ggagcccaag
     4141 agttctttcg accggacgat tccccgcttt acctggtggc cccgccgggg aacagaagaa
     4201 tgggttacgt accgtttcga cactcctcga cacgtctctg aggtgcaggt gtattggttc
     4261 gacgacaccg gcgcgggatc gtgccgggta ccggcttcgt gtcgcgtgga gtggtgggat
     4321 ggtgaaaatt ggcaacccgt tcgtcccgat cctctttgtg gcgtggaaaa agaccgctac
     4381 aatgtgctgc gatttgatcc tatgaccaca actcaattgc ggctaatggt acagctccgt
     4441 gagggcttct ctgccggcat tttagaatgg cgagtgaagt aaccagcgat cgtatgctga
     4501 catgggttta tcccctcaga caatgatccg tcgtagtatc gtggtcatca ttctaaaggc
     4561 gtgatgggtc ctctcacctg gacaaaaatg ggggactcat ttcgccggga gaaaccctgg
     4621 gtagtggttt caattttgac agtacacgat gggacaggtt tgttcgcccg ctacaccgcg
     4681 ctaagatctc gtgggcgcga aaggaggaac ggcaaatgtc cacacttcag cgatcaagcc
     4741 gccgttgtga taatctgatt tctgggtttg gacttggtgt gttccttatt ctcgggttca
     4801 ccgcagttct gtttgcgagt ggggcatccg cacaaaacat atcgtttcag cgggtggaaa
     4861 tagatgccga gccgcccaag agcccgtggc ttaagctggc cggcgacttt aatggagatg
     4921 gcaagttaga catcgccatt ggtggggcat ctatgtatct cgtatggtat gtcaatccca
     4981 catggcaaaa gatcaccatt gcggaaaaag gatggcaaac cgtatctggg gcagtgggtg
     5041 acatcgatgg ggacggtgat gttgatatcc tgcctggtgc ccaaatctgg ttggaaaacc
     5101 ctctgcccaa gggagatccc acgaagggcc catggccggt ccgtcgaatt agcaatgttc
     5161 gaacccacga tgccctcctc gcggatctcg acgttgacgg tcgattggat atcgtggcgc
     5221 gggatcagtc gggcttcaat cacaatacgg gcaacaagat ccatatgtgg tatcagcgag
     5281 atctcgatcg gtgggattat cacgtggtgg attgtccgca tggggagggc ttggccgtgg
     5341 cagatctcga ccgtgatggt gatccggatg tgatcatcgc cggtatttgg tatgaaaacc
     5401 ctggtaaggc cggtggtgac tggaagtcgc atcagtttac cgcgcagtag acctggcccg
     5461 atagcaaggt ggctgttggt gatatcaatg gggatgggcg atgcgatatc atcctgtcgc
     5521 cgtcagaata caaaggtcaa acataccaag ttgcctggta cgaagcacct tcggatcctg
     5581 cctcgccgga atgggcgcaa cacgtgatta ttcccgcaat tgaatcggtc tgtcatagcc
     5641 tgcaggtggc cgacatggac ggtgatggcc tgctcgatat cgtcacggcc cgaatgcacc
     5701 agggggcgcc accccaggaa gtgatggtcc tgctcaatag ggaaaaaggc cgaaaatggg
     5761 agaaactggt cgtgtcaact cgtgggtcac acgacctgct cgtggctgat tttgacggtg
     5821 acggtcggcc cgatattctg ggggccaacc acggcggccc ttaccagcca gtagagcttt
     5881 ggctcaatcg ggccaagtag aaaacgggca ccactggagt ggcggctaga acctctctcc
     5941 ttaacgggtg tccccgggac tccgcctact taccttatga tcttctctca tgctgaaagc
     6001 aaatggctgg gcgggtttga tttttgccga tggttgtcaa tgctgggcct ttgcttatga
     6061 tgcataaacg gtaaatagct atgttttcct gaaacctgcg gcgagagccg gattctggga
     6121 agagattgcg accgcaggat gtttttatta gcggaggctg accatggttt tgcttgagtt
     6181 ctcgatgtat ccactcgata agggaccgag tctcagtgcc tacgtggcga aaagccttga
     6241 gatcatcgac aaaagtggtc tcgactacca gtgccatgcc atggggacga tcattgaggg
     6301 agagtttgat caggtcatgg acgtcgtcaa acggtgtttt caggcgatgc aggcagagtg
     6361 cgaccgcatc gaatgcatca tcaagattga ctatcgcaaa gggcaaagcg gcaggatacg
     6421 ttccaaggtc caaagtgtgg aagctaaact gggtcggccg gtcaaaaagt gatcagccat
     6481 ctgtatggca ataaaaacag cggtttttga acggtcacga agcgttttga tgggggatta
     6541 gttttgtcag ggcctcgacg taaaaatact cgccccacat gaccgattcg tccaccccca
     6601 aattctttcg cgtgtgatag acgccatgct tgaggatgcc ctcccagccc ggcgtttcga
     6661 ttgccaaata ttcgggctgc gagagagcca tcagcatgac gagggctgta ttgcgataga
     6721 cgacgcttcg ctccggcgat gctgtctgct gagccagatc gagcaatccg ctggcggcaa
     6781 tagcggcggc tgagctttcc ttttgccgcc cccaaggtgg aggttgactc aaatcggcgt
     6841 caaaatccca atagggaacc ttgtcttcgg ggagatgtgt gatccagtat tccgcgttct
     6901 tctcggcaac ttctaggaac tcagggttct gagtgaggcc gtaaaccttg ctaaagccat
     6961 acatggacca cgccaatcct ctggcccaac aactctcggc actcagcccc tgatgcgtac
     7021 tttggccgag gaactcccct gtctccacgt cgaaaagccc ctcatgggcg gtcgagccat
     7081 cttctcgcac gagccgatcg cgcgttgtgc gacagtggtc aagcgcaatc tgcatgagcc
     7141 tgtcatcgtt ggtgtggatt gctgcataaa agacgatagg gacattcatc ataatgtcga
     7201 tgaaaaggct ctggggtccg ataaacgaac acaaataccg tcccttcggt tggtatctca
     7261 tcgcaagggt ccggcctgct gtgatgagga cctcttgaag cgcagggtcc tttgtcagtt
     7321 cataccacgg caagtaagtg ttcaaaaaga taaaccccag gtcgtggacg ttacgatcgt
     7381 gctttcgatg ttccaggagt ttggagtaat ggatggcacg ttctagccat ttctcgtccg
     7441 aggtatatcg gtagaatcgc cacatcatcc cggccaggaa tccgccggtc cagtcagtcc
     7501 acaattcgcc ttcgtgatac cacttaccct ggaccgtgta aatgggaaag aaatcagggt
     7561 gcttttcgat aaggtgttct acttgacgtt gggcaaaatc cagggcggtc ttgaatgtct
     7621 gcaattcttc cggtctgaga ttcatccatc acctccggta accttaaatc aacaaacatt
     7681 gccgatcatt atagtgccaa cgtggaaacc gcggaaccac tgtcggttat tccttccgtg
     7741 ggccttctgg gctgagtacc ggaacaaacc catgccggag aagtcggctc acgaaaagaa
     7801 tctctttacc gatgcgattg ccgtggagtt gactgggttt ctatggggca aggtatttat
     7861 cggatgtgag tacctgatga tgttcatcca tctgaaacag aatttctcgt tctttgtggg
     7921 aatgcccccg agggatgact ctaaaggcta cgtggtggac tagggaaact actcgaacaa
     7981 acgtcggcca acttttgtta caagcgacat tttccgtaac caatcgctcg acctctgctc
     8041 tgcggcgggt gttttctctt ttcagcccac attatcttct atcaccgagg ggaacttttc
     8101 tttcggcctc cccacaccgt gagccgactg gtcatgttcg tcgttgcacg tcagctctcc
     8161 cagtttctta aggcgggacg gtccccatcc tcggtagccc gaaaaagacg aatcgcctgc
     8221 ccggcaaaca cgacaaactt ggcaaatgct ttgccaattg acgtgcccgg gtcctctgtt
     8281 tatgacgcga atggtccgcc gtgggagtcc gtcggtcatg ccaagagtac gaatcccgtt
     8341 gacaattacg atctgcggag ctttgcgagg aaggctggct ggaaatggtt ccggtgggga
     8401 atgtattcct gaagttgcac aagaagttgt ggactcacca attacacgaa aggaggttgc
     8461 tgatgctgtg tgaattgacc gcccgtgtaa gtgtcctggt cgctattctc tttggcctgc
     8521 tggcggcgga tgcagcgaag gctgccgagc cggccgaccc cgacctgatc cccgaggccc
     8581 gtcgcgtttt ggagtacctt aaatctttgc agggcaagca gatgctctcg ggtatttcgg
     8641 gttcgcagga cgcgcagccg tgggcagtgt tgcatatgac ggggcgcgag ccggccatcg
     8701 ccggtggcga catggctggg tttcaccgca aatgggagaa gcagtatcac caagtgatgc
     8761 agaacacggt ggataagtgc atccattggt ggcgggataa gggaggcatc gtcgcgctgc
     8821 aataccactg gatgaagccc ggtgagccgg aaggcagtgc ctggatcggt ccgccgcgcg
     8881 gaaccggtcg gcttgatctg gcgaaggccg tcacaccggg tacggaagag caccgcgccg
     8941 tgatggccga tttgaaggtg acggccgatt acctcgagca gcttgccgag gcccgggtac
     9001 cggtgctctg gcgcccactc catgagatcg acggcggttg gttctggtgg accgacgccg
     9061 agacgcccga gaacacggcg gccctgtggc ggctgatgtt tgactacttt gtcagggagc
     9121 ggaagctcca caacctgatt tgggtctata atgcggcgca cgtgagccac acgcggaaac
     9181 ctccggcagc gacgttcgag gaagaggtcg cccatcgccg gcgatactat cccggcggcg
     9241 agtatgtaga tatcgccagt atcgacacct atgccaaccc caagctcggc tggagtgcgg
     9301 cccaggagga cgcgcgacgt cgggcttacg aactgatgca gcaggtggca ccgggcaaga
     9361 tgctggcggt ggccgaggac gccgccttgc tcaatcccga cgtcttccga cgcgagggac
     9421 ccgcgtggct ctactgctcc gcatggtgga cgggcggtaa ggagaaccca gtggagtgga
     9481 tgcggtatac cttcaaccat gaagcctatg tgacgctcga cgagttgcca cttctggtcg
     9541 agggcaacgt gatgcccaac gtgcggatca ccgatccgtc tgacgggctt gcactgaacg
     9601 ggtcgaacgt tgtcatctcg ggcttcgctt ctgatcgcaa cggcaatctt cgccgcgtga
     9661 cggtgcatgc ccttcgcggt ccgtggcgga attggttcct gcgagccgac gcggacgtgc
     9721 tgcgtgcctt ccccgattcg acccaactgg gtgaggcgag cgtcgcgccg gatggtcagt
     9781 ggacgttcac atggcaaaat gctcccagcg gggtctataa cctcgtggca ctggcccgcg
     9841 acgccgaggg tgctgtgact tgctcaaacg tcgttcgcgt gacggtgggg atacacaatc
     9901 tggcacgcgg tgtgaagagc gtggcttcat ccaccagcaa atggggtggt ccgcccgcgg
     9961 cggctatcga cggtgatccg aattcgatgt ggtggagcga caatgatcag cccgacccac
    10021 agtggcttat ggtggacctt ggtacagaac agaaggtggg agccgtctcg gtcctgtggt
    10081 ggaaggccta cgcaaaggat tacacagtcg aggtttccac cgacggaacg tcgtggcgcg
    10141 aggttgctcg cgtcgagggc cgcagcaact ggttgggcga tatggatgtc ttccgcttcg
    10201 agccgaccac ggcccgatac gtgcgccttc actgcaccag ccgagcggtg acatggcagg
    10261 cttacacggt gtatgaattg ggcgtgtacg agtcgattcc cgagtgacgt gcgcgagtta
    10321 actcgtggtg cagtgggacg actgagaaag gtgggagcaa attaatccct cccggatcct
    10381 ttgggggacg acggtctcgg tgtttatgtc gcttgttggc gttggatcgc ggaccgagga
    10441 aatcaatagg ttcctacctt tcactttggc aacaggttta ccgataacac gatgcccagg
    10501 attcaactgc tactcggaga aagtgtgggc aagcactcga cagtggcggc caacatcctc
    10561 cgcggcgttc ccatttgggt tcacatgaag tggcgaacgg agaacagagc tgaccagtag
    10621 agattttggc acgcgttgat aggatccctt taaggttcac aaggacatga tgatgaagcc
    10681 atctctgagc cgcagaagat ttctccaaga ttctggtcta aacgttgcgc taatggtgat
    10741 tagctcgcgc cagtcccaac ccccgcgccc ggccgtgagt cccaagctcg agccgtccga
    10801 gaaagtgttc ctcaagacat gggatctagt agagttgccc aatcttgcca tctccctgag
    10861 ctcgactttt gccatttttc gggtgccatt tttcgggaaa attgatcttg gcatgggagc
    10921 cgaattcgcg caaaaaagac tccttcctgg ttatgttaac tgaaaccttg cccagaaagg
    10981 agtctagaat gttgccgttg ccttccgt
//
LOCUS       DAOSUT010000136        10953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3355_length_10953_cov_9.911819, whole genome shotgun sequence.
ACCESSION   DAOSUT010000136 DAOSUT010000000
VERSION     DAOSUT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_10953_cov_9.911819"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1524
                     /locus_tag="PLD05_11895"
     CDS             220..1524
                     /locus_tag="PLD05_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOP78187.1"
                     /translation="MTETKVLNRWWVVLGAILIQLALGAIYAWSVFTKALTAEPYSFT
                     KTQTQIIFSVGLATFAFVMVLAGRLQSRIPPRIVSLLGGLMLGLGYILARFVGTSFWG
                     HVLTLGIVAGAGIGLAYVVPIAVGVRWFPDKKGMLTGLAVAGFGFGAFLWIQLAGNLG
                     QLINMLGVANVFFVYGIIFAVLVAIGSIWMVNPPEGYHPPGWDPAAQAKKAAKVGGTR
                     EFSSFQMLRTPQFYMLWFMFVAGAMAGLMVIGVIKLFGIERLQAFYSTAGEQDALRKA
                     EVTAELAMAIFFAIANGLGRIGWGIISDFIGRKASLVLMLISQTAVMAAFFWLAQTPT
                     LFFLGACWIGFNFGGNFALFPAATADLFGNKNVGANYGWVFTSYGIGGIVGPILGGYF
                     GDLAKASGRLEAWLVPFLVAAGACFLATLLAIALQPPREHRS"
     gene            1669..2742
                     /locus_tag="PLD05_11900"
     CDS             1669..2742
                     /locus_tag="PLD05_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOP78188.1"
                     /translation="MASSQDMMKSSDSTESLKPSRRDYLAMLSAGAGLGLASLLGCRP
                     GTVPQTMGQQRRLRAAFSNAGLAGTWNKLGYDAAKLWGDLLNVEVVWFDGEFNGQRQR
                     EKIELAAAEDWDFCAFQAHQIDILAEPCRRLKARGIPIIGMDTLIVAEDKLFDHGVVV
                     YVTPDHVRMAEQSTQYMVDKIGGKGKVIHIGGQSEHSGAQDRAKGFQNIISKYPEIEV
                     VGGGVRWCDWKTEKARDTFDVLLEQTPDRIAGAFFHNDDMALACVPRLKGTRHEGMVV
                     TGVDGQRPGLLGVRDGLLAATSVNPAAMIHMYALVIGQFIVRNQEKFDKLLKIVLPCP
                     LVSQESGNLDAMLYLSDPKHCLV"
     gene            complement(3078..3920)
                     /locus_tag="PLD05_11905"
     CDS             complement(3078..3920)
                     /locus_tag="PLD05_11905"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOP78189.1"
                     /translation="MATHDFDSGLRIGSHVFKSRLIVGTGKYSSFEVMQQALELSGTE
                     CITVAVRRERLFDQQGRNILDFIDTKRYTILPNTAGCFTAEDAVRVARLGREILTQLG
                     NPGADWVKLEVLGDKRTLLPDPIATVQATEILVKDGFQVLVYTTDDPIVAKRLKEAGA
                     TSVMPAGSPIGSGQGILNPNNIKICLEYLKENDPDYPVIVDAGVGTASDVTVAFELGV
                     DGVLLNTGIAHAKDPLRMAVAMKYAAEAGRLAYLAGRIPKKLYATASSPLEGTIAPSH
                     EAGK"
     gene            complement(3928..4155)
                     /gene="thiS"
                     /locus_tag="PLD05_11910"
     CDS             complement(3928..4155)
                     /gene="thiS"
                     /locus_tag="PLD05_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOP78190.1"
                     /translation="MANHPDSQTIRILINGEWREVASGITLADLLKELKLADKPVAVE
                     RNLQVVPKARHPETPLADGDRLEIVTLVGGG"
     gene            complement(4280..4885)
                     /locus_tag="PLD05_11915"
     CDS             complement(4280..4885)
                     /locus_tag="PLD05_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78191.1"
                     /translation="MTSLTRIHTWAAVFTVGVVCGVLLSTHWPQTPIRAVATDRGQNF
                     AVATGPVDAGVEAFFFLDFLTGQLRGVVLSNQTRNFQTTYEANVFADLTTVIQLKNAE
                     IQKLNAQAARSGGPTRPEIQIPQSPNYLLVTGLADIRRGPSVGVRPGQTMVYVTETNT
                     GIVLAYMVPWSPERHSANQPFSTPLQLWAAEQFSTVVLRTE"
     gene            complement(5146..6315)
                     /locus_tag="PLD05_11920"
     CDS             complement(5146..6315)
                     /locus_tag="PLD05_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78192.1"
                     /translation="MNISAKLIAALTVLAGLYAVADAQSPGKKDTLRVFYFGNSLTAS
                     SMPELHEELGRSAGKVWKCDAFLGAGWQSWQHRNELWRAMGRPLDAATQSASSRGDLT
                     IDESLVKSAPMKPKAFLSGEWDAIVIQIFGSRLHYVTDEMWGMKFNGPIDVGDVAAAS
                     DIIRIFLKKNPQGRVYIYTVWPNMPSGKVPPDDQLPDWAKEMKKRFGSIRSAEFPDRA
                     GFDYAAQWEKPYQGDFDRPWIGHISRTRDYTNQVFEGIKRNFPELWDQRRLACIPAGE
                     LFFELDKRMQAGHMPGITTIKDFYTDVQHIRAGLPAYSIAALFYTVLFEEEPDSLDFT
                     IYNDPQKYGPDPHHDRGELLPITPERAKVVNETIWALVQRHPFTHLAQHSEAEKR"
     gene            complement(6335..9292)
                     /locus_tag="PLD05_11925"
     CDS             complement(6335..9292)
                     /locus_tag="PLD05_11925"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOP78193.1"
                     /translation="MRTSLQLIFVLCVAAKGFAETGNLGRDAAPISPAEHQARFNYTR
                     VQPLFETPMRDCAITRGPDGFFFLTGTTGTPRQDGTINFAVNDGIRLWRSHDLKVWED
                     LGLVAPLSLLKAKVEDLGLLRTGRYEDEWKGFIAPELHFVKGGVYLTYALRPCGTGLL
                     KSKSGKPEGPYEDVGLITRVGTDASLFEDDDGTVYWVFGGGWIARINDALTELAEEPR
                     LIQPSTPNANTPGGKILQVGTAGAFLFKRNGVYHLLAAAIHGRLGVPCYDTFVATASS
                     LDGPWTQRKVAVPHGGQSTMFEGPEGQWYATFSGVDSRAALRERPAIVPVDWVDSVMY
                     FYPKNEPWPWKRPEVITEAWGWENARPLSDLRYRDPYGVNGRDGYFYVSGLHSPGSHG
                     RRVELLRGKDLTGGEPWEPFPIPGFETLDRVPWFNEKAEANFDVSLCKPFYAAGTFWI
                     NLWVRGGKRLLRSESGTMQGPWKVAVDPPPSPSAPVGYWCAHPFQDHRGDMYGYLYAT
                     LWPMNSTFTQLEADRKPPVEDGYEPMFDRRLRGYVRESSDGSHLLRGDAPIGHVFWID
                     GHYLMMGGCDWHGEYRRFGTYDSAVYWAHEIGGPWHPNRSVLPHCGNSGLFQDDNGTW
                     WVVSFANDNFLPDHSRLRCLPLEIKWNGNGYDIRPKHKEANPYVHREEAIVPDAQAER
                     IAPAYPVIKLPEDIILQSPSITLVKEEGSAVYYLTGTAAVETATGLKPGSRTSKPDFH
                     NNDGIYLWRSTDLKTWVALGKVFDLATVNRKASKASGWNSPFGVYFTPPDSLEPRYDR
                     AVVTPKLYRIRNDYWIVCSCGRQQVCLLKSQSGNPEGPYDMEGPPSLHGSTAAILDSG
                     SPTESRTDGYMFAYDPSLFVEDDGTIYLVFGPGWIAKLHEDLGRGLAETPRLLEIEGP
                     RLYAGTGGCALRKEAGKYILTALTEWNDTVEYVADSLYGPYRNPRLVLAQSRHVGFFK
                     SIDGEWTAVVSSY"
     gene            complement(10139..>10953)
                     /locus_tag="PLD05_11930"
     CDS             complement(10139..>10953)
                     /locus_tag="PLD05_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOP78194.1"
                     /translation="VSVNITRKESFLREFGSQAKINSPQKRHPKNGKSRAKEVATMIV
                     ADDNFASIVAGVEEGRIAYDNVRKVIYLLISTGAAEVMMFLTAILTGLRIPLLPVQLL
                     WLNLVTNGIQDVALAFEGGEPGAMKKKPRRPNESIFDSQMVWQTVVSGLTIGGLAFGL
                     WYWLKAFQGLEETAARNLVLLLMVLLQNVHVFNCRSETTSAFRVPFRRNPLLIAGVLI
                     AQGVHILSMYLPPMQKVLQVAPVTLAQWLSLLVLALVILVVMETFKWLRAKA"
BASE COUNT         2392 a         3124 c         3002 g         2435 t
ORIGIN      
        1 aaggatcgcc caatacgatg aacgcggtcg aggctcaccg gcgcggtgtc gagtatgttc
       61 gcaaactctt ttcctgaaga taccctcggg ccgctcgcga aaggctatca ccggaaaatg
      121 ccgtcaagca ttccttcaac aaacagcgcg aaaatggcaa tcgtacgaac taagaaagcg
      181 taagaacatt tttggtgacc gaattcttag gaggctccga tgactgagac gaaggtactg
      241 aatcgctggt gggtggtctt gggcgcaatc ttgatccaat tagccctggg cgcgatttac
      301 gcttggagtg tgttcaccaa ggccctgacg gcagagccgt attccttcac caagacacaa
      361 acgcagatca tcttcagcgt tggattggcg accttcgctt tcgtcatggt tctggctgga
      421 cgccttcagt ctaggattcc tccccggatc gtgtctttgt tgggaggact catgttggga
      481 ttaggttaca tcctggcacg ctttgtgggg acgagctttt gggggcatgt cctaacgttg
      541 ggaatcgtgg ccggggcggg catcggtttg gcctatgtcg tgcccatcgc cgtcggtgtg
      601 cgatggttcc ctgataagaa aggaatgctc acaggtctgg cggtggctgg tttcggcttt
      661 ggggccttcc tgtggatcca gcttgccgga aatcttgggc aactgatcaa tatgctgggc
      721 gttgccaatg tcttcttcgt ttacggaatt atcttcgccg ttcttgtcgc gatcggatcg
      781 atttggatgg ttaacccacc ggagggctac catccgccgg gatgggatcc agccgcgcag
      841 gccaagaaag cggcgaaggt cgggggaacc cgcgagttca gttcgttcca aatgctccgt
      901 accccccagt tctacatgct gtggttcatg ttcgtagcgg gggccatggc aggtttgatg
      961 gtcattggcg ttattaaact cttcggcatc gagcgtcttc aagcctttta cagtaccgca
     1021 ggcgaacagg acgctctccg caaggcagaa gtcaccgccg aactggccat ggccatcttt
     1081 tttgccatcg ccaacggact gggccgcatc ggctggggaa tcatttcgga cttcattggg
     1141 cgtaaggctt cccttgttct tatgctcatc tctcaaacgg cggtgatggc tgcattcttc
     1201 tggctggccc agacccctac gttattcttc ctcggggcgt gttggattgg tttcaacttc
     1261 ggggggaact ttgccctctt cccggcagcg acagctgacc tattcggtaa caagaatgtt
     1321 ggggccaact acggctgggt gtttacctcg tatgggatcg gcggaatcgt tggacccatt
     1381 ctcgggggat actttggcga cttggctaag gccagcggtc ggcttgaggc atggctggtt
     1441 ccgtttttgg tagcggccgg ggcctgcttc cttgccacgc tgttggctat tgcactccag
     1501 ccgccccgag aacacagatc gtagtccgcc ttcaatccgg cttggggccc accacttcgc
     1561 caaacagggc cgcatcgcat tggttgattt tttggcgtga tccattacag tgatagacgc
     1621 gccctgcgtc acactgtggt gggaggacgt cagcgcttgg aggtcaatat ggcaagttcc
     1681 caggacatga tgaagtcttc cgattcgacc gaatctctca aaccatcgcg tcgcgactat
     1741 ttggcaatgc tctctgccgg ggcggggctg gggttggcgt cgcttctagg ttgccgaccc
     1801 gggacggtcc cccagacgat gggccagcag cgtcgattac gggcggcctt ttccaatgct
     1861 ggtttggccg gcacctggaa caagttaggc tacgatgccg ccaagctatg gggcgatcta
     1921 ctcaatgtcg aagtcgtttg gttcgatggg gaattcaacg gtcaaagaca gcgtgaaaag
     1981 atcgaacttg ccgctgctga agactgggac ttctgtgcct ttcaggctca ccaaatcgac
     2041 atcctagccg agccatgccg acgccttaaa gcccgcggca tccccatcat cggcatggat
     2101 acgctcatcg tggccgagga taaactcttc gatcatggcg tggtcgttta tgtcacgccc
     2161 gatcacgtcc ggatggctga acagagcacg cagtacatgg tggataaaat cggcggcaag
     2221 ggcaaagtca tccatattgg cggtcaaagt gagcacagcg gggcccaaga tcgcgccaaa
     2281 ggttttcaga acattattag caagtaccca gagatcgaag tcgtcggtgg tggtgtccgt
     2341 tggtgcgatt ggaagaccga aaaggcccgc gataccttcg acgttcttct cgagcaaacc
     2401 ccggaccgca tcgcgggggc tttctttcac aatgacgaca tggccctcgc ttgcgtccca
     2461 cgactcaaag ggactcgtca tgagggaatg gtcgtgaccg gcgtcgatgg ccagcgccca
     2521 gggttgttgg gcgtgcggga cggcttgcta gccgctacga gcgtcaatcc cgcggccatg
     2581 atccacatgt atgccctagt catcggtcag tttatcgtcc gcaatcagga gaaattcgac
     2641 aagctgctca agattgtcct gccgtgtcct cttgtctcgc aggaatcggg caacctggac
     2701 gcgatgctgt acctctccga tcccaaacat tgcctcgttt aacaagccaa gcacgaaatg
     2761 gcagacgcgg cggacgccat ggaccgcgtc cctccccagc ggtccaaggg gaaaaagatc
     2821 ggaggcggag gaccgcgtcc ctcccagtta acagtctatc cccaaagtcc ccctctccct
     2881 ccgggagagg gttggggtga gggcaagcga acgtccgaat cgattgagaa aaccggcagc
     2941 gatttgaggc actctcaccc ttgactcctc tcccgctcaa cgggtgacgg gaggttgtgg
     3001 gataggctct aaggagaaac acggcggacg cggaggacgg cgtccctccc tgtaaaggcg
     3061 aaattgcaat caaaccccta tttgcccgcc tcatgggatg gagcgatcgt cccttccaag
     3121 ggactgctgg ccgttgcgta cagctttttc gggattcggc ctgccaggta cgccaatcgt
     3181 ccggcctcgg cagcgtattt catggcgacg gccatccgca gtgggtcctt ggcatgggca
     3241 atacccgtat tgagaaggac cccatcaacg ccaagctcaa aagccaccgt tacgtcactg
     3301 gccgttccca cgccagcatc cacgatcacc ggatagtcgg gatcgttttc cttcaaatac
     3361 tccagacaga ttttgatatt gtttgggttg agaatccctt ggccggagcc gatgggactt
     3421 cccgccggca tgacactcgt cgcccccgct tctttcaatc gcttggccac aatcggatca
     3481 tccgtggtat aaacgaggac ttgaaagcca tccttaacca aaatctcggt ggcctggacc
     3541 gtggcgatcg gatcgggaag aagcgttctt ttgtcgccca gaacctccag ctttacccag
     3601 tccgccccag gatttcccaa ttgggtcaga atttcccggc ccaaacgggc gacccgcacc
     3661 gcgtcttccg ccgtaaagca tcccgccgta ttggggagga tggtgtatcg cttggtgtca
     3721 atgaaatcga ggatgttgcg cccttgctgg tcgaacagtc gttcccgccg cacggccacg
     3781 gtgatgcact ccgtccccga aagctccagg gcttgttgca tcacctcaaa ggaactgtat
     3841 ttccccgttc caacgatcag ccgactttta aaaacatgcg agccgatccg aaggcccgaa
     3901 tcaaaatcat gcgtggccat gccaaaatca acctccaccc accagggtta caatctcaag
     3961 tcggtctccg tcagccaacg gtgtttcggg gtgcctcgcc ttgggcacaa cctgaagatt
     4021 cctttccaca gccacaggct tatcggccaa tttgagttcc ttcaatagat cggccaaggt
     4081 gatgccggaa gccacctccc gccactcgcc gttaatcaaa attcggattg tctgagaatc
     4141 cggatgattt gccataggga cacccgacca ttgtgtgatt caatctcaac gcgcccccta
     4201 acctcatact acgacaaagc cacgccccga gatcaacccc gggacgtggg ctttcaaatc
     4261 atcgcacaca agcaccgaat cactcggttc gcaacacaac cgtgctgaat tgctcggctg
     4321 cccacagttg taggggtgtc gagaacggtt gattagccga atgccgttcc ggtgaccagg
     4381 gcaccatgta ggcgagaacg ataccggtgt tcgtctccgt gacataaacc atggtttgtc
     4441 ccggtcgaac acccactgac ggaccacgtc ggatatcggc caagccggtt accaacaaat
     4501 aattcggtga ctgcggtatt tgaatctccg gccgggtcgg tcccccactt cgagcggctt
     4561 gagcgttgag cttctgaatc tctgcattct tcaattgaat gaccgttgtc aggtcggcaa
     4621 acacgttggc ctcataagtc gtttggaaat tccgtgtttg attagagagc accacccccc
     4681 gcagttgccc ggtgagaaag tccaggaaga aaaaggcctc caccccggca tccaccggac
     4741 cggtggccac cgcgaagttc tgcccacgat ccgtggctac cgcccgaatg ggtgtttgcg
     4801 gccagtgcgt gctgagcaac actccacaca ccaccccaac cgtgaacaca gccgcccaag
     4861 tgtgaatgcg cgttaaagac gtcattgtcc agactccgcg agcttccagg caccgacaca
     4921 gttcaccacc acctcaaaca acagcctttg ataggtttat ggtatcgttt ctggcatgct
     4981 ctcgccagac gtgttttggc taacacaaag aggaatgttc cctttttcaa acagcgagca
     5041 acatcactat gggaaccaaa agacgcaaat cccgactgat cgcgtgacat gctttgccgt
     5101 tggaaacaac aggaggggag tcgattcagg cacgcacccg ctatttcaac gcttctctgc
     5161 ttcggaatgt tgagcgagat gcgtgaacgg gtgccgttgg accagcgccc agatcgtctc
     5221 gttgaccacc ttggcccgct cgggcgtgat gggcagcaac tcgccccggt cgtggtgcgg
     5281 gtcaggccca tatttctgcg ggtcattgta gatggtgaaa tcaagcgagt cgggctcttc
     5341 ttcaaacagc accgtgtaaa acagagcggc gatactatat gcgggcagac cggcacggat
     5401 gtgctggacg tcggtataga aatccttgat cgtcgtgatg cctggcatat ggccagcctg
     5461 cattcgctta tccagttcga agaacaactc gccggccgga atgcacgcca gccgacgctg
     5521 gtcccagagt tctggaaagt tccgtttgat gccctcgaac acctggttgg tgtaatcgcg
     5581 ggtccgagag atatgtccga tccagggcct gtcgaaatcg ccttgatatg gtttttccca
     5641 ctgcgcggcg taatcgaatc cggcacggtc cggaaattcg gcggaacgga tgctgccgaa
     5701 tcgttttttc atttctttcg cccagtccgg caattggtcg tcgggtggga ctttgccgct
     5761 cggcatgttc ggccagacgg tgtagatata taccctgcct tgcggatttt tcttgagaaa
     5821 gatacggatg atgtcgctgg cggccgccac atcgcccacg tctatgggtc cgttgaactt
     5881 catcccccac atttcgtccg tgacataatg gagcctggaa ccaaagattt ggatgacaat
     5941 cgcatcccac tcaccgctga gaaacgcctt tggtttcatc ggtgcagact tgacgagact
     6001 ctcgtcgata gtcaggtcgc cccggctgga agcactttgc gtggcagcat cgagtggccg
     6061 tcccatggct cgccacagtt cgttacggtg ttgccacgat tgccagcccg cacccaggaa
     6121 ggcatcacac ttccagacct tgcccgcgct gcgtcccagt tcctcatgca actcaggcat
     6181 ggagctggcg gttaagctgt tgccgaaata aaacacacgc aaagtgtctt tcttgcctgg
     6241 cgactgcgca tcggcaactg catataggcc ggcgagaacg gttagagcag caattagctt
     6301 cgcggaaatg ttcatatcct ttactccttg tcggtcagta actgctaacg actgcggtcc
     6361 actcgccgtc tatggactta aagaaaccta catgtctgga ctgcgccagt accaggcgag
     6421 gattgcggta aggtccatac agcgagtcgg cgacgtattc caccgtatca ttccactcgg
     6481 tcaatgcggt aagtatgtac ttaccagctt cttttcgcag cgcgcagcca cctgttccag
     6541 cgtagaggcg agggccttcg atctccagta accgcggcgt ttctgccaaa ccccggccaa
     6601 ggtcttcgtg cagcttggca atccaaccgg gaccgaagac gaggtaaata gtgccatcat
     6661 cctccacgaa caggctgggg tcgtaggcga acatgtagcc gtccgtgcgc gactccgttg
     6721 ggctgccgga atccaaaatg gcggcagtcg agccgtgcag ggatggggga ccctccatgt
     6781 cgtagggccc ctccgggttt ccgctttgac ttttcagcaa acacacctgt tgccggccgc
     6841 aggaacagac aatccaatag tcattacgga tgcggtaaag cttgggggtt accacggcac
     6901 ggtcgtagcg gggctccaag ctatcgggcg gggtgaaata gacgccgaac ggactattcc
     6961 agcccgacgc ttttgaggct ttacgattga ccgtagctag gtcgaacacc ttgccgagcg
     7021 caacccatgt tttcaggtct gtggatcgcc aaaggtaaat gccgtcgtta ttgtgaaaat
     7081 cgggtttcga agttcgtgac cctggtttca gccctgttgc ggtttcgacc gccgccgttc
     7141 ccgtgagata gtacaccgct gacccttctt ccttcacgag ggtaatcgac ggtgactgga
     7201 ggataatgtc ttctggcaat tttattaccg gatatgccgg cgcgattctc tctgcctggg
     7261 cgtcgggaac gatcgcctct tcgcgatgga cgtaaggatt ggcttccttg tgcttcggtc
     7321 taatgtcgta tccattgccg ttccatttaa tttccaatgg aaggcagcgc agccttgaat
     7381 ggtcaggcag aaaattgtcg ttggcaaagc tgacaaccca ccaggttccg ttatcatctt
     7441 gaaacagccc ggagttgccg caatgtggca atactgagcg gttgggatgc catgggccac
     7501 cgatttcgtg cgcccaatag acggcactgt cataagtccc gaagcggcgg tattccccgt
     7561 gccagtcaca gccgcccatc attaagtaat ggccatcgat ccaaaaaacg tggccgatcg
     7621 gcgcgtcacc acgcaatagg tgagatccgt ccgaggattc gcgaacgtaa ccccggagcc
     7681 gtcggtcaaa catgggttca tatccatctt cgacaggtgg ttttcgatcg gcctcaagtt
     7741 gggtgaacgt actgttcatg ggccacagag tcgcgtaaag gtagccgtac atgtctcccc
     7801 tgtgatcttg gaagggatgg gcacaccaat agccaacagg ggcggatgga gagggagggg
     7861 gatcaaccgc aaccttccaa gggccttgca ttgtgccaga ctcgctgcgg aggagccgtt
     7921 tgccccctcg aacccaaaga ttgatccaaa acgtaccggc agcataaaac ggtttacaca
     7981 aagaaacatc gaagttagcc tcggcttttt cgttgaacca cgggacccgg tcgagtgtct
     8041 caaaaccggg gatcggaaac ggctcccacg gttcgccgcc ggtcaggtcc tttcctcgca
     8101 acaattccac gcggcgccca tgcgaaccgg gactgtgcaa acccgaaaca tagaaatacc
     8161 cgtcgcgacc atttacaccg tagggatctc ggtacctgag gtcactgagt gggcgggcgt
     8221 tttcccaacc ccacgcttcc gtaatcactt ctggtctttt ccatggccaa ggctcatttt
     8281 tcgggtagaa gtacataaca ctgtcgaccc agtccactgg gacaatggcc ggccgctcgc
     8341 gcaaggccgc ccggctgtcc actccgctga aggtcgcata ccactgcccc tctggtccct
     8401 caaacatcgt ggattgaccg ccatgcggga cggcaacttt gcgctgggtc caagggccgt
     8461 caagcgacga ggcagtggcc acaaacgtat cgtagcacgg tacacccaag cggccatgga
     8521 tagcggcggc cagcagatgg taaacaccat ttctcttaaa caaaaatgcc cccgctgtcc
     8581 ccacctgcaa aatctttccc ccgggcgtat tagcattggg cgtgctcggt tgaatgagcc
     8641 ggggttcctc tgcaagttcc gtcagggcat cgttaatccg cgcgatccac cccccgccaa
     8701 agacccagta aacagttcca tcatcatcct caaacagcga ggcgtccgta ccgacccttg
     8761 taatcagccc cacatcctcg tacggccctt cgggtttgcc tgacttactt ttgagcagcc
     8821 ccgtcccaca cggcctcaac gcgtaggtta gataaacgcc acccttgacg aagtgcaact
     8881 ctggtgcaat aaaccctttc cattcatcct cgtaacgccc ggtccgaagc aaccctaagt
     8941 cttccacttt cgccttcagc agcgacagcg gcgcaacaag ccccaagtcc tcccatacct
     9001 tcaggtcatg ggaacgccac aaccgaatgc cgtcatttac cgcgaaattg atcgtaccgt
     9061 cttgccgagg ggttccggtc gtcccggtga ggaaaaaaaa cccatcaggc ccgcgagtga
     9121 tggcacaatc ccgcatcggt gtttcgaaca gtggctggac acgggtgtag ttgaacctcg
     9181 cctgatgctc cgccgggctg atcggcgcag cgtctcggcc cagattgcca gtctcagcaa
     9241 atcccttggc cgcaacgcat aggacgaaga tgagttgtaa tgacgtgcgc acgtcgattc
     9301 tcctcgcatg gttgtgtagg gactacttgc ggtttggtac tcggtgaaac tccacagtac
     9361 tcaggcccca ttttgcaccc tgattctttg gtaagacaag ctagttcccc acctttaccg
     9421 agaggcgctc aggaaccttg tcttcgcggg tatccaattg atttttcttg tgcggtcagg
     9481 atacggggct tttggggtga aggtaccaga ataggccgag tggatgaggc acccgtcgtg
     9541 acaggcataa cggacacaga acatccgggc aacccggcca agccattttc cgacagccac
     9601 cggaacgtta agccgccgcc gggacaccgg tcttacgtaa gttaggcaag ccagaagaga
     9661 tgttccctgt gcggacggct tcaaggggaa tagccgtggc tcgctatttt ggaaactgac
     9721 aagcagaaat catggcaatt tacccgactc ggtgattttg ccaaactttg acacggatgt
     9781 gaccttgtgg tttagtacta tgggactaca gtcatatatt gtgtagatgg gtcaagccgc
     9841 agaacaatct tttcgcggat tgttttgaaa agacaacact cctagctgca gggaggagca
     9901 ttaccaaagg aagcattcga gcgcacgggt aggggcagga catctcttgc ccctacggtt
     9961 ggcctgagct gttgccggaa tattaccaac gggatcgccg agaaaaagaa acgcattgtt
    10021 ttgcgcgagc gggtatgggt tattgcgcgc gaccaggagg gcggcgatca accccatcac
    10081 caatcacctc acgcttttcc gtaatcgctc gccgcatttg ccaaaatggc cgcaaatgct
    10141 aggctttggc cctcagccac ttgaacgtct ccattaccac caggatgacc agtgctagaa
    10201 ccaacaggct caaccattgg gcaagggtca caggcgcgac ctggagaacc ttttgcattg
    10261 ggggcagata catgctcaag atgtgaacgc cttgagcgat caagactccg gcaatcagga
    10321 gaggattacg acgaaacgga acccggaaag cggatgtggt ctcggaccgg cagttgaaca
    10381 cgtgcacatt ttggagcagc accatcagta acagcacgag gttacgagcg gcggtttcct
    10441 ccagaccttg aaaggccttc agccagtacc acagtccaaa ggccaggccg ccgatggtca
    10501 atccggaaac aaccgtctgc cagaccatct gggaatcaaa gatggattcg ttgggtctcc
    10561 tgggcttctt tttcatggca cccggctcac cgccctcgaa ggccagagct acgtcttgaa
    10621 tgccattggt aaccagattg agccagagaa gttgcacggg tagcaatgga atacgcaaac
    10681 cggtcaagat cgctgtcagg aacatcatga cctcagcggc gccggtggat ataaggagat
    10741 aaatcacttt gcggacgttg tcgtaagcaa tgcgtccctc ttcgacaccg gcgacgatcg
    10801 aggcgaagtt gtcatcagca acgatcatcg tcgccacttc cttagctcga cttttgccat
    10861 ttttcgggtg ccgtttttgg ggagaattga tcttggcctg ggagccgaat tcgcgcaaaa
    10921 aagactcctt cctggttatg ttcactgaga cct
//
LOCUS       DAOSUT010000137        10854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3389_length_10854_cov_9.919252, whole genome shotgun sequence.
ACCESSION   DAOSUT010000137 DAOSUT010000000
VERSION     DAOSUT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10854
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_10854_cov_9.919252"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLD05_11935"
     CDS             <1..308
                     /locus_tag="PLD05_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78195.1"
                     /translation="RLAPAILSKRNTRELRLSISPEVRIAAFSTEYEEFMYVFAVNYD
                     SGLKTAEVTFHIPSVDDSQSATVEVIDEDRSLPLRDGQFTDTFAPLAVHIYRLRRPR"
     gene            484..690
                     /locus_tag="PLD05_11940"
     CDS             484..690
                     /locus_tag="PLD05_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78196.1"
                     /translation="MQDVGTQVALALIPLPGYFAQFPGSTISYYQPVAYASRKGYDFP
                     QNVELVLEHEGVFHVDRQYLVGLA"
     gene            706..1755
                     /locus_tag="PLD05_11945"
     CDS             706..1755
                     /locus_tag="PLD05_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOP78197.1"
                     /translation="MLPIPWRNRGTMPLAAIVGLLVITGGWDVRGSNQSLRAEEKLGW
                     IERADPRLDALIDSDAVVEKLAEGFDWSEGPVWVPRDNCLLFSDVPQNVVYRWREGQG
                     IDVFLKPSGYTGSVARGGESGSNGLALDREGRLLLCQHGDRRVARWENGRFVTLADRY
                     QGRPLNSPNDLVVKSNGDIYFTDPPYGMTAESRRDPNALPFCGVYRISRTGELTLLVS
                     DMTRPNGIAFSPDEKTLYVAQSDPERPLWMAFPLKEDGTLEEGKVFFDAKPWLEAGLK
                     GLPDGMKVDVHGNIFATGPGGVNVFTSDGTFLGRIRITVPAANCAFGNDGSVLYITAD
                     MYLLRVKTKTKGLGF"
     gene            1900..2283
                     /locus_tag="PLD05_11950"
     CDS             1900..2283
                     /locus_tag="PLD05_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78198.1"
                     /translation="MQRKEILAKVAAGELTPEEADRLLAELDSESAVLTCKVSPKGAV
                     SVYGLQRMPVTLYADQWHRLLAFGDDIRRFIEEHREELRWRVRPEPQVETQGEPPKES
                     TVTTDRSVASESGVNPDSLESQGCD"
     gene            2524..4083
                     /locus_tag="PLD05_11955"
     CDS             2524..4083
                     /locus_tag="PLD05_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78199.1"
                     /translation="MKMLTKDWRRPLAITVVVAWSTLVLGKAQKVKADEPIPVYQIAQ
                     RRERLPEIPPASAVCFSSRWFHPVNRDDPHDTFKAAEAFHATDFVWTYSLDPKFVGRL
                     RQMGHRVFLAVNSMVPDPPDFRRRLRGRIVDLENRPVTAPWMRSWADPAWGCANSPEY
                     REGFIEYAVRAVEAGATALQMDDPRMNMAAVAWGACFCPHCMESFRHFLKQRVSKETA
                     TEWGIGNLDTFDYRQYLIERKAPVGDAFAKYDGGELKKLFTEFQTESVGQFFSYVRAE
                     IDRRVGRHIVFSSNNYRGSWEFPYNLFELGMAELPQREATPMTLYERYREAAIRGKHQ
                     IFTLVPQTADGSEVPITRRTIAACYALGGHLIVPWDVYTGPQKPRYYGKPEDYADLYK
                     FVRDFAQWFDEYEEAAFVFDGFHDERYSGHPPLRVDATQVMGVVRAKPQDPAAPVVIH
                     LVSWQDTPSPFVIRIDPRQFPSGVSKHILLLLPGQAPRKITPTEHGNAEISVEIPALN
                     PWGVLLLGAGP"
     gene            4184..6391
                     /locus_tag="PLD05_11960"
     CDS             4184..6391
                     /locus_tag="PLD05_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOP78200.1"
                     /translation="MMTSVAKHRMSFGHAPARARLFILFLAFSLSPALGAAQIKILEF
                     QPDVPVCRAERLITLHLTLGNDGARDEQGTVELHLPPVVELIKGNRNTPFAFRDGAGE
                     LTLSWTVQASQEGRHELAVEVKPVSGSPVRQTLNLLFLPSVTIEKADRIPDPQPVATD
                     ILVGAHHCPLWEKDRPEIWRNVIRHPERTPALGFYCQEQPEVADWETKWAVEHGISFF
                     VYCWYRASQGGPVETRLSSAIHEALFHSRFQDKIRFAIMWENQRRGVAGIANEEDFLQ
                     NLLPYWIENYFRRPNYLIIDRKPLLFIYRPEYLIQDLGSVEKVAQAFAKARELCRQAG
                     FDGIYLLGEYRGTNPKHLQLMKDLGLDYTFAYVWPVPNNPSPEEAIRAQLDYIKKTQE
                     LNILPQVITVSQGWSGWRDEGSIWKLPPEDFERLMREAKAFAKTLPPEQLGSRIILLD
                     NWNEWGEGHYIGPHREFGFGYLDAVRRVFAGNAAPHLDLLPEDIGLGPYDLPIRTYYQ
                     RERESRQKARTWVKPIVSDEALVAYWTFDENKDEVAAFDYSGHRLGGILHQIERVKGW
                     RGTALDCRGGSMIVPTDERLSDWAQMSVECWIRTDQPDQDNRWIINRVFGGAETSGFR
                     LGILRGRPSFQIPVTAWSHHLVGKEPLPQGRWVHIAGTFDGTTMRLYMDGHEIGRMER
                     SGSVRNNNRPVVVGNYDVDHTAHFVGELDEVRLYSRVLTPEEIASRAAQPAEK"
     gene            6477..7229
                     /locus_tag="PLD05_11965"
     CDS             6477..7229
                     /locus_tag="PLD05_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOP78201.1"
                     /translation="MGPIFGDLVTILLAGLVSGIICRQLRISGLVGFIIAGIMIGRGG
                     LNLIASHGKELEQIAQTGVLLLLFAMGIEFSLAELAGLSRHMVLAGTTQVLLVTVPLL
                     VVFLLLEVSIERAIVLSAAVAISSTVLAYRTLTEWGQASSPIGRRALGISLFQDLLLI
                     PSLLFMPCYSGARSTGLISALVLTGMAVVFTAAVPLAHYVFEKWIVPFLIRVKSVELT
                     MLFVLVILLMACWLAQVFGLPPALGALAAGSV"
     gene            7226..>8113
                     /locus_tag="PLD05_11970"
     CDS             7226..>8113
                     /locus_tag="PLD05_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOP78202.1"
                     /translation="MSETRLTKQLDALVFPFRETFGVVFFVSLGSYIDWRILWRDPLW
                     FLIAIPLLLALKSVAGGVALRISGLTWRTSLGAASLLVPMSELSFVLLSEAARSQILT
                     GTEYEKMLMVALASMIVSPELFRIGLRVLRSDSSKRPTSMGFDVPSVDVNKAIVVGLG
                     PIGSMVASQLELRGMDVGLIDQSPVNLQPFAQQGFRTVCGDARDPEILLRAGGQEAHL
                     AMICVPDDTVAGTITRNLRQINPNCTIVVRCQYQSSRKTLMKAGAQIVISEEAVTSEV
                     LRNFIMTSVFNLSGESPLAS"
     assembly_gap    8114..8213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8661..10064)
                     /locus_tag="PLD05_11975"
     CDS             complement(8661..10064)
                     /locus_tag="PLD05_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOP78203.1"
                     /translation="MDGRSWPLYAAAVTGLFFSTIIVGLVSAQGVIPYQTMDPAYLVA
                     TEKPPQIAETNDDLTQRVADLEKALAQIKEKEQAAAKKSAGQMSVNLSGRFFIDSVFF
                     GQSDGSMAAFGDAQDTVQLRAARLGAFGKGFDVFDYQIVADFASRDGSNSQRTSLKDA
                     YIGVDDLPWLGRVQVGHFKEPFSLEELTSARFITFMERASFISLFVPSRNLGIAAFRV
                     SENERMTLAFGGFREVGEKPPYVADDDGGYAFTARVTYLPWYDEATQGRGLLHLGLGY
                     SFRDVDNPMRQLRARPEVNEGPYLLDTGVFGNVEDYQLLNPEMAFVYGPFSVQSEYLA
                     AFYNRAGGEDPTFHGVYVQTGYFLTGEHRFYNRKKGAFERVKPFENFFRVRTSDGNVE
                     TGLGAWELAYRYSWLDLDDKDVLGGTMADHSFGINWYWNPYMRLMFNYVHAQVSPPRD
                     ATNADFNVFQFRAQVDF"
     gene            10653..>10854
                     /locus_tag="PLD05_11980"
     CDS             10653..>10854
                     /locus_tag="PLD05_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78204.1"
                     /translation="MLTPELESNAENENGYNQSWAAYPVVGRAAGWASITTIYTSPRR
                     LWHPKKDLDMAVHMHREMDRLRE"
BASE COUNT         2424 a         2887 c         2969 g         2474 t
ORIGIN      
        1 cccgtctggc acccgctatt ctgagcaagc gaaacacaag agaacttcga ctaagcattt
       61 cacccgaagt tcgcatcgcc gccttttcca cggaatatga agagttcatg tatgtgttcg
      121 cagtcaatta cgattcaggt ctcaagacgg cggaagtgac gtttcacatc ccttctgtag
      181 atgactcgca atcagcaact gtggaagtca ttgacgagga tcgtagcttg cccctacggg
      241 atggacaatt caccgatacc tttgcccctc tggcggtgca tatttaccgc ctccgcagac
      301 caagataagc cacagcggac ggggaagtgg agtacgcaaa tcgggatttt gttggaagca
      361 attcccagcc gcataaaatg gtagtcaatg agaccacgtc cttttgcggc aataggattg
      421 cggccccgcc tggagttggg acgcgtgggc tcaaaaatat cgtccaccga atacccgtag
      481 ccgatgcaag acgtcggcac gcaagtagcc ttggccctca ttcctcttcc tggatatttt
      541 gcccagtttc cggggtcaac aatttcctac taccaaccgg ttgcttacgc aagtcggaag
      601 ggttatgatt ttccgcaaaa tgttgaactt gtcctggagc acgaaggagt gtttcacgtc
      661 gatcgtcagt atcttgtcgg gcttgcgtag agccggaggt gtgcgatgct accaatacca
      721 tggcgaaacc gagggactat gcccctagca gcgatcgtcg ggcttcttgt catcactggc
      781 ggatgggacg ttcgaggtag caatcaaagt ttgcgagctg aagagaaact tggttggatt
      841 gaacgcgctg acccgcgttt ggatgccctg attgactcgg atgccgtggt cgagaaattg
      901 gcagaaggct tcgactggtc agagggccct gtgtgggttc ctcgagacaa ctgtttgctt
      961 ttctccgacg tgccacaaaa cgttgtatat cgctggaggg aagggcaggg catcgacgtt
     1021 tttctcaaac cgtcaggtta taccgggagc gtcgcacgcg ggggcgaatc ggggtccaac
     1081 ggactggccc tggatcgcga aggcagactc ctcttgtgtc agcacggcga tcgtcgggtt
     1141 gcccggtggg aaaatggccg ctttgtcacg cttgccgacc gatatcaagg gcggccactc
     1201 aatagcccca atgatttggt agtcaagtca aatggcgaca tctactttac agatccacct
     1261 tacggaatga cggcggaatc acggcgcgat cccaacgcct tacctttctg tggggtatat
     1321 cgcattagtc gaacgggcga actgacgctc cttgtcagcg acatgacccg gccgaatggc
     1381 attgcgtttt cccccgacga gaagacgctt tacgtggccc aatccgatcc ggagcgtcct
     1441 ctttggatgg catttccgtt gaaggaagat ggaacattgg aagaagggaa ggtctttttc
     1501 gatgccaagc cgtggctgga agcaggattg aaaggactgc ccgacgggat gaaggttgat
     1561 gtccacggaa atattttcgc aacgggtccg gggggagtga atgtcttcac ttcggatggg
     1621 acgtttctgg gaaggattcg catcacagtt ccggcggcaa attgtgcctt cggcaacgac
     1681 gggtctgttc tttatatcac tgcggatatg tacctcctgc gagtgaaaac taagacaaaa
     1741 ggtcttggat tctgaggctg cggtggccaa acggccgttt tctttgtggc ggtttgagag
     1801 gcgaccgatg catggccgga gctaggcggc ttaagagtta ctgagatgta ggaactcgtt
     1861 agcagaggaa gacctattct gtgaaggaga cgatgaccca tgcaacgaaa agagattctc
     1921 gcaaaggtgg cggcgggcga actaacgccg gaggaagctg accggttatt ggccgagttg
     1981 gattctgagt ctgccgtgct cacttgcaag gtaagcccta agggcgcggt gagcgtgtac
     2041 ggtctgcaaa ggatgcccgt cacgttatac gcggaccaat ggcaccgcct tttggcgttc
     2101 ggagatgaca tccgccgctt tattgaggaa catcgagagg aattgcgctg gcgcgttcgt
     2161 ccggaaccgc aagttgagac acaaggcgag cctccaaagg aaagcaccgt cacaactgac
     2221 aggagcgtgg catcggagag cggagtaaac ccagattcgt tggaatctca aggttgcgat
     2281 tgatttttag ccgcagtgct ccacccaaac gattttggtg gaaggacgcg acctttgctc
     2341 aaagcgattc tttcccatca agaaccaagg gttcctcggg tggtggcgtc aaccgaccga
     2401 tctccgacct gttgaatgag gccgggatct accaacattg ggacaggtcc attctcagag
     2461 tgtcccgaag tctcctttac atccctttac caaaacttcg acgaaaagac ccgaaagggc
     2521 ttgatgaaaa tgctgacaaa agattggcgt cggcccttgg caatcacggt ggtggtagcg
     2581 tggtccacgc ttgtcctggg taaagcacaa aaggtcaagg cggacgagcc tattccggtt
     2641 tatcagattg cccagcgtcg ggaacggttg ccggaaattc ccccggccag tgccgtgtgc
     2701 ttttccagcc gttggtttca ccccgtgaat cgcgacgatc ctcatgacac tttcaaagcg
     2761 gccgaggcgt ttcatgccac cgattttgtt tggacctatt cgcttgatcc caaatttgtg
     2821 ggacgtttga gacaaatggg ccatcgtgta ttcctcgccg tgaattcgat ggtcccagac
     2881 cctccggatt ttcgtcgccg cctccgcggg agaatcgtcg atcttgaaaa tcgtccggtg
     2941 acagctccct ggatgcgttc ctgggccgat ccggcttggg gttgcgcaaa cagcccggag
     3001 tatcgagaag gcttcatcga gtacgcggtt cgcgccgtcg aagcgggggc aacggctcta
     3061 cagatggacg atcctcgaat gaacatggca gccgttgcct ggggtgcgtg tttctgtccg
     3121 cattgcatgg agagcttccg acacttcctc aagcagcggg tatcaaagga gacggctacg
     3181 gaatggggaa tcggcaatct cgatacgttc gactatcggc agtacctgat tgagagaaag
     3241 gcccctgtag gcgatgcctt cgcaaaatac gatggtggtg agttgaaaaa gctgtttact
     3301 gagtttcaaa cggagtctgt ggggcagttc ttctcctacg tgcgggcgga aatcgatcgg
     3361 cgggttggtc gccacatcgt cttctcttcc aacaactacc ggggaagctg ggaatttccg
     3421 tacaacctgt ttgagctggg gatggccgaa ctccctcagc gcgaggcaac tcccatgaca
     3481 ctatacgaac gctaccgcga agccgccatc cgtggaaagc accagatttt cactcttgtc
     3541 ccacaaaccg ccgacggctc agaagtcccc atcacccgac ggacaattgc cgcgtgctat
     3601 gccctgggtg ggcatctcat cgtaccttgg gatgtttaca ctggtcccca gaagccgcgt
     3661 tactacggca agcccgaaga ttatgccgac ctttacaagt tcgtacgaga ttttgcccag
     3721 tggtttgacg aatatgaaga ggcggccttc gtcttcgatg gctttcacga tgagcgatat
     3781 tctggccatc cacctctgcg cgtggatgcg acacaagtga tgggtgtggt tcgggcaaag
     3841 ccacaagacc cagccgcccc ggtggtcatc catttggtca gttggcaaga cactccctct
     3901 cctttcgtga tccgcattga ccccaggcag tttccaagtg gggtatccaa acacatattg
     3961 ctcttgttac ccggccaggc ccctcgaaaa atcacgccca ccgagcatgg caacgccgag
     4021 attagtgtcg agatacccgc cttaaatcca tggggagttt tgctgctcgg agcgggaccg
     4081 taggttaatc ctttaatgtg gttccgctgt ggggtagggg atatcatttt ctctaagtgt
     4141 gaagccggcc attcaagaat tgcagtctca aaggaacgcg atcatgatga cgagcgtggc
     4201 gaaacataga atgagctttg gtcatgcccc cgctagagcg cggttgttta tccttttcct
     4261 tgccttttca ctaagccccg cactcggagc ggcgcagatc aagattctag aatttcagcc
     4321 ggatgtaccg gtctgtcggg cagaacgact tatcactttg cacctgacac tgggcaatga
     4381 cggcgctcgt gacgagcagg gaactgtcga acttcatttg ccccctgttg tggaacttat
     4441 caaaggaaac cgcaacaccc cgttcgcgtt tcgcgacgga gcaggggagc ttaccctgtc
     4501 ttggacggtg caggcatcgc aggaaggccg gcacgaattg gcagtcgagg tcaaacccgt
     4561 aagtgggtcg ccggttcggc aaacacttaa cctcctcttc ttgccatcgg tcaccatcga
     4621 aaaagcggac agaatcccgg atccacagcc cgtggccaca gacatccttg tgggtgcgca
     4681 tcattgtccc ctgtgggaga aagataggcc cgaaatctgg agaaacgtca ttaggcatcc
     4741 cgaaaggaca ccggctctgg ggttctactg ccaggagcag cccgaagtcg cggactggga
     4801 aacaaaatgg gccgtcgaac acggtatttc gtttttcgtt tactgttggt accgtgccag
     4861 ccaaggcggg cctgtggaaa cccgactgtc cagtgccatt cacgaagccc tatttcattc
     4921 gcgattccaa gacaagataa gatttgccat catgtgggaa aatcagcgtc ggggcgttgc
     4981 aggcattgcc aacgaggagg acttcctgca aaacctctta ccgtattgga tcgaaaatta
     5041 ttttcgccgg ccaaactatt tgatcattga cagaaagcct ctcctcttca tttatcgccc
     5101 agagtatctc atccaggact tgggcagcgt tgagaaggtc gcccaggcct ttgccaaggc
     5161 ccgtgaactc tgccgtcaag ctggatttga cgggatttat cttttgggtg aataccgcgg
     5221 aacaaacccc aaacacctgc agcttatgaa agacctgggg cttgattaca cgtttgccta
     5281 cgtgtggcca gtccccaata acccgagtcc tgaggaggca atccgcgccc agctggacta
     5341 catcaagaag acgcaggaat tgaatatctt gccccaagtg attacggttt cccagggatg
     5401 gagcggctgg cgcgacgaag gttccatctg gaaactgccg ccggaggatt ttgaaaggtt
     5461 gatgcgggaa gccaaagctt tcgccaagac cttgccccca gaacaattgg gaagtcgcat
     5521 catcttgttg gataactgga acgagtgggg cgagggacat tacattggcc cccatcgaga
     5581 attcggcttc ggttatctgg atgctgtccg tcgtgttttt gccggcaacg ctgcaccgca
     5641 tcttgaccta cttcccgagg atatcggcct tggtccctac gatttgccga ttcgtacgta
     5701 ttaccagcga gagcgggaaa gtcggcaaaa ggcccggact tgggtgaaac cgatcgtgtc
     5761 tgacgaagcc ttggtcgcct attggacatt cgacgaaaat aaagatgagg tggcagcctt
     5821 tgactattcc ggccatcgcc tgggagggat actccatcag atcgaacgcg tgaaggggtg
     5881 gcgggggacg gctctcgatt gccgcggagg ctccatgatc gttcccaccg atgagcggct
     5941 ttcagactgg gcgcagatgt cagttgaatg ttggattcgc accgatcaac ctgatcagga
     6001 caataggtgg atcatcaatc gggtgtttgg cggtgccgag acatcgggat ttcgcttggg
     6061 gattctccgg ggaaggccca gttttcagat tcccgtgacg gcttggagtc atcatttggt
     6121 gggaaaagag cctttacccc aaggccgctg ggtccacatt gctggcacgt ttgatgggac
     6181 cacgatgcgc ctctatatgg acggtcacga aatcggtagg atggagcgat ctgggagcgt
     6241 ccgcaacaac aaccggccgg tcgttgtggg caactatgac gtggaccaca cggcccactt
     6301 tgtgggcgaa ctggatgagg tacgtctgta tagccgcgtg ttgacgccgg aggagatcgc
     6361 ctcacgagcc gcccagcctg cggaaaaatg agccagacaa aaaggaattt aggctagaat
     6421 gggtatattc ccgacaaaaa gtctcttcgt gcatcgtgag ggcgaaaggt tgtagcatgg
     6481 gacccatttt tggagatctt gtcacgatcc tgctagccgg tcttgtctca gggattatct
     6541 gccggcaatt gcggatttct ggcctcgtcg gttttatcat cgcgggtatc atgattggcc
     6601 gcggagggct gaatcttatc gccagtcatg gcaaggaatt ggaacaaatt gcccagacgg
     6661 gagtgctttt gcttcttttt gccatgggaa ttgagttctc actggctgaa ttggccggcc
     6721 ttagtcggca catggtcctg gccggtacca cacaggtgct cctcgtgacg gtccccctcc
     6781 tcgtggtgtt tttgctttta gaagtttcca ttgaaagagc catcgtattg agtgcagcag
     6841 ttgccattag ctcaacagtg ctcgcttatc gcacacttac ggagtggggg caagcaagct
     6901 cgcccatcgg tcgtcgagcg ctaggcattt cattatttca ggacctccta ctcattccct
     6961 cgctcttgtt tatgccgtgc tattccggcg cccgctccac ggggttgatt tctgctttgg
     7021 ttctcaccgg catggccgtt gtcttcactg ctgctgtgcc cctcgctcat tatgtctttg
     7081 aaaaatggat tgtcccgttt ctgattcggg ttaagagcgt tgagctaact atgcttttcg
     7141 tcctggtcat tctcctcatg gcttgttggc tggctcaggt attcggtttg cctccggcgc
     7201 taggtgccct cgcggccggg tcagtttgag cgaaacacgc cttacgaaac aactggacgc
     7261 actggtcttt ccgtttcggg agaccttcgg tgttgtcttt tttgtgagcc tcggtagcta
     7321 cattgactgg cgaatcctct ggcgagatcc cctttggttc ctgatagcca ttcctttact
     7381 cctggccctc aagagcgtgg ctggcggggt cgccttgcgg atttccggcc tcacctggag
     7441 aacctcactg ggagcggcat cgctgctggt acccatgagc gagctctcgt ttgtcctcct
     7501 ctccgaagct gcccggagcc agattctcac aggcactgaa tacgaaaaga tgctaatggt
     7561 cgcactagca tctatgatcg tctcacccga actttttcgg atcggcttgc gtgtgttgcg
     7621 cagcgactcg tcaaaacgac cgacgagcat gggattcgac gtccccagcg tggatgtcaa
     7681 caaggccatc gtggtgggtc ttgggccgat tggcagcatg gtggcgtcgc aactggaatt
     7741 acgcggtatg gacgttggcc tgatcgacca aagtcctgta aatctccaac cgtttgcgca
     7801 gcagggattt cggaccgtgt gcggcgatgc ccgtgatcct gagattctcc ttcgcgcagg
     7861 tgggcaagag gctcacctag ccatgatatg tgtcccggat gacacggtgg ccggaacgat
     7921 cacacgcaat ctccgccaaa tcaaccccaa ctgcacgatt gtcgtgcggt gtcaatatca
     7981 gtcttcccgc aagaccctca tgaaggcagg tgcccaaatt gtaatcagtg aggaggccgt
     8041 gacgagcgag gtgctcagga acttcattat gacgtcggta ttcaatcttt ccggagaatc
     8101 tccacttgcg agtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacgccct
     8221 tgggttggca ggcaccactt tgtgagtcgc acgcttggcc ctcacagccc agtgagggat
     8281 cgcacgtccc acacctgccg ccgtgttgat tgccacaccc acacgggcac cggggcagca
     8341 tggtgcatac atcgtgcacc tccacttccc aacaatgctt ggtgatcttc tcaataccac
     8401 attgaacttg acaaattttt tgctggcatg gccccggctt gccacaaatg gcgcatcggc
     8461 ccggtgaacc gtaaacgtcg gtttgcgacg attcaagcgt ccagccggta ctggcaataa
     8521 gagttacaaa caccacgcaa gccgcagcag ttttcatgtt catgcctgca tctctctttt
     8581 atctccaaac gtgagggaaa cctcgcgtcc gtcgctttgc ttggtattct tgaaactatc
     8641 ctgctggtcg atggagacgc tcagaaatct acttgcgcac gaaattgaaa cacattgaag
     8701 tctgcatttg tggcatcgcg gggtggactg acctgcgcat gcacatagtt gaacatgagc
     8761 ctcatgtagg gattccaata ccaattgatg ccaaaactgt ggtccgccat agtcccgccc
     8821 aagacatcct tatcatccaa gtctaaccac gaataacgat aggctagctc ccaggccccc
     8881 aagccggtct ccacgttgcc gtcggatgtc ctcactcgga agaaattctc aaaaggtttg
     8941 acacgctcaa acgcgccttt tttgcggtta taaaatcgat gttcgccagt caggaaataa
     9001 cctgtctgaa cgtacacccc gtgaaatgtc ggatcttctc cgccagctcg gttgtaaaac
     9061 gccgccagat actcagactg gacagagaaa ggtccataaa caaacgccat ctccggattg
     9121 agaagctggt aatcttccac attcccgaaa acgcccgtgt caaggaggta tggcccttcg
     9181 ttcacttcgg gacgtgcacg aagctgccgc atggggttat cgacatcccg gaagctatag
     9241 ccaagaccaa ggtggagcaa acctcgccct tgagtcgctt cgtcatacca gggcaagtag
     9301 gtcacgcggg cagtgaaggc gtagccccca tcgtcgtccg ccacgtaggg aggtttttca
     9361 ccaacctccc ggaatccgcc gaaggccaga gtcattcgtt cgttttcgga aacccgaaag
     9421 gcagcgattc ccaaattgcg agacggcacg aaaagggaaa tgaaactggc acgctccatg
     9481 aaagtgataa aacgagctga cgtcaattct tcaagactaa agggctcttt gaaatgaccg
     9541 acctggacac ggcctaacca agggaggtca tcaacgccga tatatgcgtc cttaagcgac
     9601 gttcgttggg agttggaacc atcccggctg gcaaagtctg ccacaatctg ataatcgaaa
     9661 acgtcgaacc ctttgccaaa tgcccccaac cgcgcggctc gaagctgaac cgtgtcctgg
     9721 gcgtcgccaa acgcagccat actgccgtcg ctctgaccaa aaaacacact atcgatgaag
     9781 aaccggccgc tcagattcac cgacatctgg ccagcggatt tctttgctgc ggcctgttcc
     9841 ttctctttga tctgagcaag ggccttttcc agatccgcca cccgctgcgt caggtcgtcg
     9901 ttcgtttccg caatttgagg cggcttctcg gtggcaacca gataggcagg gtccatagtt
     9961 tggtaaggaa ttacgccttg ggctgagaca aggcccacga tgattgtcga gaagaataac
    10021 cccgtgaccg cagcagcata aagcggccaa cttcgaccat ccatgagcag gtctcctctg
    10081 caatgatgct ggtgcgaaac gccaaggcgg tcatgcgtgc ggctcttgag tccacaggca
    10141 cgccggaaaa tcttctccaa cccgcaaatt ttttcagttc caaaggatcc tttactcact
    10201 taatagagac ggataccatg cgcctcctcc gaagtggcat cggccttttc ggaaccaatt
    10261 tccaactaag cgcccgcaaa ggccaaaaga cccgcacatt tcttattttg aaaagatgcc
    10321 gtatttatcc cacactgagc attttccgca taaatagggc gtcattggta aattgtcagt
    10381 tcccatggcc tgtcgcagat ctgcgctcgt aagccgacta agagcctatc cgaaaaagta
    10441 ccccctctcc ctccgggaga gggttggggt gagggcaagc gaacgtcgga atcgattgag
    10501 aaaaccagca gcgattcgag ccaccctcac ccccgacccc tctcccgccg agcgggcgag
    10561 gggagattat gggataggct ctaaaaagga gccgtcgtct ccgtcataag gcctcaaacc
    10621 gagggcaacg ggaagctgtc gcctatcccc gggtgcttac accggaattg gagtcgaacg
    10681 cggaaaacga aaacggttat aatcagagtt gggcagcgta tcccgtcgtg ggtagggccg
    10741 ctggctgggc gagtatcacc acaatttaca caagtccaag gcgactgtgg catcctaaga
    10801 aggacctcga catggccgtt cacatgcacc gcgagatgga caggcttcgg gaaa
//
LOCUS       DAOSUT010000138        10785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3414_length_10785_cov_8.673439, whole genome shotgun sequence.
ACCESSION   DAOSUT010000138 DAOSUT010000000
VERSION     DAOSUT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_10785_cov_8.673439"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLD05_11985"
     CDS             complement(<1..764)
                     /locus_tag="PLD05_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOP78205.1"
                     /translation="MWLAPWFLVGGLLASVGVVIIHLMHRRRYRVVHWAAMDFLLQAS
                     RRSRRMIELRDWLLLLLRVACVSLFTVGMARPHWGNRPITSASAMHLVVMIDNSLSMG
                     YKTLGRSLLDDAKQKAKELIRSLSPESRVTIIPFPDPAELRDLGVLCTKDEAQSLIDS
                     LKIVDQKGDMAAALQQAVQACARFPQPKQKRLVIISDMQRNNVPSGGLSQILAKLPGP
                     IEIIRIRSGSAANTWVEDVSLPDRFLIPGAETRFRAL"
     gene            complement(1066..>1769)
                     /locus_tag="PLD05_11990"
     CDS             complement(1066..>1769)
                     /locus_tag="PLD05_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78206.1"
                     /translation="LHRRFGTKFVLNIYWTTGDEFSLDKFPDQYKGEWQDNADWLKLA
                     FHAWSDKPDRPYEDAPPEKLLADFDRVSNEILRFAGELTYSPTTIIHWGMVRPEAFKP
                     LASRGIKVLSGYFRQMGGRWDVNYRLSDEISEYLSKHDAWVDYDSGITFSKIDIVCNT
                     VPREEIPAVLQSAESDPNQAEVMDLMTHEQYFWPFYTRYIPDHFQRLETAISWVSERG
                     YRPVFLHEGFLGAPE"
     assembly_gap    1770..1869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2426..3064
                     /locus_tag="PLD05_11995"
     CDS             2426..3064
                     /locus_tag="PLD05_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOP78207.1"
                     /translation="MAKLRLTRLLIVVASCAVMMCSLLSIPGSDGIAEEERPSLPEHR
                     LTPQPFPIAVIDVATVLENLPQFRSEQMVLRADVERARSHFQERQNALVQQQQELQKV
                     SPDTPDYKEKIARLQQEISQLQTEFQLQQQEFFNRERQLLLGAYKQIEQAVDQIARER
                     GFVLVLRTSREPSGNNPQELLSYLGREVVWADATIDITKDVVAKIRSTPSKD"
     gene            complement(3113..4024)
                     /locus_tag="PLD05_12000"
     CDS             complement(3113..4024)
                     /locus_tag="PLD05_12000"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOP78208.1"
                     /translation="MTTNRILPTILAGVILWYVAGSAVVLGEDGAQSEAPKDPAVAKQ
                     SKQQIDEMELRGGKNFIPTKVYSAEEDAAILEMFQGLRVADVCDAMDQVGLRNVGVMS
                     PEIRPAWKDPVNLSHRFVGVAITVRYVPHNMVVPSQLSPEEFNQWSGKWYANLSPEPF
                     VELIRPGTVVVIDDANGADVGSIGSNNIMGWKKRGCVAVVTDATARDLDEIALERIPL
                     YFRGPGRGIRPGRNLIESVNRPVEVGGVLVRPGDIVLGDADGVIVVPREYAEQVAAYA
                     RATLEKDKAARRNLYRALGIPDDVSVQ"
     gene            complement(4124..5350)
                     /locus_tag="PLD05_12005"
     CDS             complement(4124..5350)
                     /locus_tag="PLD05_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOP78209.1"
                     /translation="MSQSASAAMPQMSPEEAVRRVAQARELLLKEVHKVIIGQDEMIE
                     QMIICIFARGHCLTIGVPGLAKTLTVSTLARAMHLKFSRIQFTPDLMPSDITGTEIID
                     VDPTTGKRSFRFVHGPVFANIVLADEINRTPPKTQAALLQAMQEYEVTVGGKTYPLEQ
                     PFFVMATQNPIEQEGTYPLPEAQLDRFMLSINITYPTRAEEREIVMATTQLTKQEIQP
                     VLTGRDILWIQQLVRQVPIAQHMVDYAVDIVRATRPKEPPSPDFVKNWLAWGAGPRAA
                     QNIVLTAKARAILHGRFAVTADDIRAMVFPVLRHRIFTNFNADAEGVDVDQILEKILE
                     VVPEPTYGEKLPVSSRSPRKAVAKSPSPPEPPPASAPVTETPLVEPPPAAPIATPPVA
                     RPVPIVPPAGKEGGTK"
     gene            5454..7757
                     /locus_tag="PLD05_12010"
     CDS             5454..7757
                     /locus_tag="PLD05_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOP78210.1"
                     /translation="MRQSRKDQRGATISCAYASWKSCFSLIHDKSIVLALLVVFLGVL
                     HCKESPGQNFRRAGQEFAFLRPIELPGGTGGTIGVVEFYHHGQIDPQGRNVIVAAGND
                     LVPVRILQLGPGDFCRLAFQVTSARSPYEILYGGPALPDDRRPTWTSEDGLLLEVREY
                     RDCNLNSFESVKAAFESSRPLGADYVSGVFHAGHPFPGPPGPYMSRYSGMLHIAQTGV
                     YGFYTSSQDCSFLLIDGKVVVSAPGRHPPSYQARPGLRKDIQFTAGAHRFEYYHAATT
                     EVGIAVAAWEVNPKGDQPAPAVIPGEAFRANLIMRGRAGPPMSPAQRFLPDFEFVIVG
                     EVPLPDRDLPLLAVRFTDKSPPPLVSSAKIEWDFGDGQTSQGLAVDHVFLRPGVFTVK
                     ETVTRAGRPFEIAQRVWIGSPRTQQKGKPLQLDDLLPVLETYQPEKLDAQSLLQFVTA
                     FLTKADQYLPPPPPNTVFVDEEEAKASSAADKLDDKTREAERRKYLQKALDAAVLGLE
                     KGQQKGDQDILAIVRNVAPIARNYFADSKKALGLWTTASERLETSEAKLEAYLNIADI
                     CLNDLVDVATAKDWLDKAATMVGGQGRQDALAALYQRLMGDYWAAKGDQEAAIKAYRE
                     AERLVADRRTEIERTAWRGAHSRSVEQFLKSGELDRAAAELRLWLDEFPGDTIEGYLP
                     LLSGKYWFARQRYEVVCALADRMLIVNPYSPYIEELLLLSARSDVARGQPERAKATLE
                     SLLKDYPGSPFVGEAKELLQKLGTGKP"
     gene            7801..>8372
                     /locus_tag="PLD05_12015"
     CDS             7801..>8372
                     /locus_tag="PLD05_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78211.1"
                     /translation="MLCRTDRLAWVTIVSVCLAIGGRSGLSLSAEVTLRIRNAPPLQL
                     VGLIQRWDRDGNPVRPVDPKAKIDSPQCISGRQRNQETWVFEGLSPGRYDAVLLGNER
                     IRIDGFDFPPVLEFDPFFRGDTEVDEEHRKTIEEDIRASRHYENKVEPLYFGGNDEVV
                     RVLVMLIRDLPTSYEADFPGAATIRHEIWQ"
     assembly_gap    8373..8472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8473..10228
                     /locus_tag="PLD05_12020"
     CDS             <8473..10228
                     /locus_tag="PLD05_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78212.1"
                     /translation="AAVAIEPPMAAKLFPAKTAVFFSIPDVPELKRAVLASSSGQMLQ
                     DPQLRPFLTDLWGGIVEAAAVVEKETGVSLEAILAIPKGELSVGLVSSEKQGWVVLIF
                     MDAKENIETLKKLIDRGREAAVKAGAILKEEDVNGLPLTVLRPRERQGNEDNRRPQPL
                     VAYAINGATLLAGLAVLGEEDLDVIRDVVGRLSGTSSSTDTLSGQSKFNQFLSSIRHA
                     TRGKIHTLLFADPIEVLRGYAKIETGAAVALAILPVLGLDGLNSLGGAGSAEISGWDY
                     VREFHVFLDNPRRGALDVPVFKPANLEPEEFIPSSVITYATVSLDLPTTVSRLAKLYD
                     GFQGDGAFERRIGGRTERFLGVNLTQEFLPATTGRISLVRWVEQPVTVTSISNGLIVE
                     LKDEDVAAMIVDRIYQTRQANFEKKEFGTWQYYQSTRTRPASLPATVRFPVPCLAALG
                     RFLIVTDSPALMQELFATTSDPPRALVESLEFKVVRGRIKDLAGRDEVSMLVFQRPDE
                     AVRVFYEGVVNQPITEAMRTSAESNPLLKAFIAARERNALPPFEVLQRYYAPQGALLI
                     DDATGLHLIDFGLRKKLP"
     gene            complement(10166..>10785)
                     /locus_tag="PLD05_12025"
     CDS             complement(10166..>10785)
                     /locus_tag="PLD05_12025"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78213.1"
                     /translation="PYRMFTSRAEYRLRLRHDNADRRLTPLGRELGLVSDERWRRFQE
                     KLDGIHRLRAILESVKYHGVPLIKRLAQPETTWDELVQICPELAAFRQDVVEQVMYDV
                     KYEGYLAREEAAIERQRRLAEKRIPSDINYDDVPHLRAEAREKLSKIRPVDFAQAGRI
                     SGITPADLAVLAVYLEQRNRRSKAPSRELLTQSEVDQVQPGGIID"
BASE COUNT         2307 a         2836 c         3054 g         2388 t
ORIGIN      
        1 agggcgcgga aacgggtctc tgcaccggga atgagaaagc gatctggcag cgacacgtcc
       61 tccacccagg tattcgcggc cgagccagat cgaatccgaa tgatttcaat cggcccgggg
      121 agcttagcca gaatttggga aagacctccc gaagggacgt tgttccgttg catatccgag
      181 atgatgacga gccgtttctg cttgggctgt ggaaaccggg cacacgcctg gacggcttgc
      241 tgaagggcgg ccgccatatc gcccttttga tcgacgatct tgaggctgtc aatcaaactt
      301 tgtgcctcat cctttgtaca caagactcct aggtcacgta gttcggcggg atccggaaag
      361 gggatgatag tcacgcgact ttcaggcgac agggaacgaa tcaactcctt ggccttttgc
      421 ttggcatcgt cgaggagtga ccttcccagc gttttgtagc ccatgctcaa actgttatca
      481 atcatgacca cgagatgcat cgctgacgcg gaggtgatcg gccggttgcc ccagtgcggt
      541 cgcgccattc caacggtaaa caaggagacg caggcgacgc gtaacaaaag taacagccaa
      601 tcgcggagtt cgatcatgcg acgactgcga cgtgaggctt gaagtaagaa gtccatcgca
      661 gcccagtgaa ctacccgata gcggcgccgg tgcatcagat ggataattac gacacccacc
      721 gatgccaaaa gaccccctac aagaaaccag ggtgcgagcc acatcagcgg attactacct
      781 tgttgtcgtt gtcagggcaa ccacccatca agttctgcgc aaacctctga tgggtgaagg
      841 gattccataa tttggcgcaa gggaggcgat atactctgtt ccggttttac gaagacgtac
      901 attttgtagc gtactcacgc taagaatctt acgcaaggtg ggaaaaattc gctacgagag
      961 gccctcgcgg tacgaaagtc cacaatgatt gccccctggc atcacaccgg tgcgattaac
     1021 ttcctgtcaa aaaagatgtc aacgacctgc gccgtgggac atgaactact cgggagcacc
     1081 gagaaaacct tcgtggagaa agaccggccg atatccccgt tcgctgaccc agctaatagc
     1141 cgtctcaaga cgctggaagt gatccggaat atagcgggtg tagaacggcc agaagtattg
     1201 ctcgtgagtc ataaggtcca tcacctcagc ctgatttgga tcgctttcgg ccgactgaag
     1261 aaccgcggga atctcttctc ggggaaccgt gttacaaaca atgtcaattt tggaaaacgt
     1321 gatcccgctg tcgtaatcaa cccacgcatc atgctttgag aggtactcgc taatttcatc
     1381 gctgaggcga taattgacgt cccagcgtcc gcccatttgt cggaagtagc cgctgaggac
     1441 cttgatgcct cgactcgcta atggcttgaa ggcctccggc cggaccatac cccaatggat
     1501 gatcgtcgtg ggggagtagg tcagctcgcc ggcgaaacgc agaatttcgt tagagacgcg
     1561 atcaaagtcg gccagcagtt tctccggcgg ggcgtcctca tacggccgat cgggtttgtc
     1621 agaccaggca tgaaaggcaa gtttgagcca gtccgcattg tcctgccatt cgcccttgta
     1681 ctgatcggga aacttatcga gggagaactc gtcgccggtc gtccagtaga tattcaacac
     1741 gaactttgtc ccaaaacgac ggtggaggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnc ggcggttgag gaccgccccg tgccgcggct gttgaatctt cacaggactg
     1921 gtttccaggg cttgggcggt cctgcggttc catgtcggca gtgttcctag accaataaga
     1981 gttgccgctt gcaagaactg acgccgatgc atcatgatgc tccttaactc tggaggccat
     2041 gccaccgcgc cgtgccctag tccggctgcg cgaaccgctc tcgtttgtcc gacagtttag
     2101 cttcttgtta tcgggagtcg caatcacccg gctagggatt tgcgaaggga atgtctcccg
     2161 aagcgagcag gcgccgattc tcttggatac ctgtaaggtc acaaccattt ccagatatgt
     2221 tcacctaacg cgttagggca atttcacgtt ttaaactggc gccgttggcc aacatgggtg
     2281 gcggaaccca ttacaatgct agtattccgg cgaagcctca gtccgtgcct ccaagaagtg
     2341 gcgacgaaaa acacggaccg aaccggccga ttattccaag gacagtttct gatagaaccg
     2401 cacacttcta gtgaggagat aagtcatggc taagctgcgt ctcactcggc tactgatagt
     2461 ggttgcgtcg tgcgcggtta tgatgtgttc gctcttatca ataccgggtt ccgatggcat
     2521 agctgaggag gagagaccga gcctgcccga acatcgctta acgccccagc ctttcccgat
     2581 cgccgtgatc gacgtggcaa cggtcctgga gaatctgcca cagttccgca gcgagcaaat
     2641 ggtgcttcgc gcggacgtgg aacgggccag gtcgcatttt caagagcggc agaatgcact
     2701 tgtgcagcag caacaggagc tccaaaaggt ctcgcctgat acgcccgact acaaagaaaa
     2761 aatagctcgc cttcagcagg agatttccca acttcaaacg gagtttcagt tgcaacaaca
     2821 ggagtttttc aaccgggagc gccaactttt gctgggggct tacaagcaga tcgaacaagc
     2881 cgtcgaccag atcgcacggg agcgcgggtt tgtcctcgtc cttcgaacca gtcgagaacc
     2941 ctccggtaat aacccgcaag aattgttgag ttatctggga agggaggtcg tctgggccga
     3001 cgccaccatc gacatcacaa aggatgtggt tgccaagatt cgcagcacgc catcgaaaga
     3061 ctaatttgcc aaaagtcacg acttttgctg ggatcgcata ttcgccgagc ccctattgca
     3121 ctgaaacgtc gtctgggatg cccaacgcgc gatagaggtt ccttcgtgcc gctttatcct
     3181 tttccagtgt cgctcgggca taggcagcga cttgttcggc atattcccgc ggaaccacaa
     3241 tgacaccatc ggcatcgccg agaacaatat ccccgggccg gacgagcacg cctcccactt
     3301 ccacaggtcg gttgaccgat tcaatgaggt ttcgcccggg tcgaatccca cggcctggcc
     3361 ctcgaaaata caaggggatg cgttcgagtg caatttcgtc caaatcacgg gccgtagcat
     3421 ctgtcaccac ggctacacag ccgcgcttct tccagcccat gatgttattc gacccaatcg
     3481 aacccacatc cgctccattg gcatcgtcta taaccaccac agttccggga cgaatgagct
     3541 cgacaaatgg ctcaggcgat aggttcgcgt accacttgcc agaccattgg ttgaactctt
     3601 ccggggaaag ctgcgatggc actaccatat tgtggggcac ataacggact gttatagcca
     3661 cgccgacaaa acgatgactc aaattgaccg ggtctttcca ggccggccga atctcgggac
     3721 tcatgacgcc cacgtttcgc agccccacct gatccatggc atcacagaca tcggcgacac
     3781 gaaggccctg aaacatctcg agaatggcgg catcttcctc ggccgaatac accttggtgg
     3841 ggataaaatt ctttccgcca cgaagctcca tctcgtcaat ctgttgtttc gattgtttcg
     3901 cgaccgctgg gtccttcggg gcctcgctct gagccccatc ctctcccaag acgacggcgg
     3961 atcccgccac gtaccagagg atgacaccgg cgaggatagt cggcagtatt ctgttcgtag
     4021 tcatgcttgt tacctcccaa agacggggcc cattttggcg gaccattcga gcccccaacg
     4081 gacggctacg acagtatggt atgacgaagt cattctgttg cactcacttg gtgccacctt
     4141 cctttcctgc cgggggaacg atcgggaccg gcctggctac cggcggtgtg gcgatgggcg
     4201 ccgccggtgg tggttcgact aatggcgttt ctgtaacagg ggcacttgct ggcggaggct
     4261 ctggcggtga cggggacttc gccaccgcct tccgtggact tcgagacgat accggcagtt
     4321 tctcgccgta ggtgggctcg ggaacaacct ccaggatctt ctccaaaatc tgatccacat
     4381 ctacgccttc ggcatccgca ttgaagttgg taaaaatgcg gtggcgcaac acgggaaaga
     4441 ccatggcccg aatatcatcg gcggtgacgg cgaaccgtcc atgcagaata gcacgggcct
     4501 tggccgtgag gacaatgttc tgtgcggctc tcggcccggc cccccaagcc aaccaattct
     4561 tgacaaaatc agggctcggc ggttctttcg ggcgggtggc acgaacgatg tccacggcgt
     4621 aatctaccat gtgctgggcg atagggacct gccggacgag ttgttgaatc caaagaatgt
     4681 cgcgacccgt caaaacaggc tgaatttcct gtttggtgag ttgggtcgtc gccataacga
     4741 tctcgcgctc ttctgcccgc gttggatagg tgatattgat ggaaagcatg aatcggtcga
     4801 gttgagcttc gggcagcgga tatgtccctt cctgctcgat gggattttgc gtggccatga
     4861 cgaaaaacgg ctgttccaac gggtatgttt ttcccccaac cgttacttcg tattcctgca
     4921 tggcctgtag caacgcggcc tgtgttttgg gcggggttcg gttaatttcg tcggctaaga
     4981 cgatgttggc gaacacggga ccatggacga agcgaaaact ccgtttccct gttgttggat
     5041 ccacatcgat gatctcggtg cccgttatgt cgctgggcat gaggtcgggc gtgaactgaa
     5101 tgcggctgaa ctttaagtgc atggcccgcg ccaaagtgga gaccgtcagt gtttttgcca
     5161 gaccgggcac accaatggtc aagcaatgtc cgcgggcaaa gatgcagatg atcatttgct
     5221 cgatcatctc atcttgtccg ataatgactt tgtggacttc cttgaggaga agctcccggg
     5281 cctgggctac gcgacgaacg gcttcttcgg gcgacatctg cggcatcgca gccgatgctg
     5341 attgcgacat gattcgagac ctccttccat cttgcggtct atctacctaa attagtttat
     5401 ggctctgtac ggatcaacgc cgatcccgtc tttgactggg aaggaaccaa ttcatgaggc
     5461 aatcgcgaaa ggaccaacgg ggtgcaacaa tttcgtgtgc ctacgcgtcg tggaagagtt
     5521 gcttttcgct gatccacgat aagtcgattg tcttggcctt gctcgttgtt tttctcggcg
     5581 ttctccattg taaagaaagt cctggccaga atttccgcag ggcaggccaa gagttcgcgt
     5641 ttcttcgtcc catcgagtta cctgggggaa ctggtggcac catcggggtc gtggagttct
     5701 accatcacgg ccaaatcgat ccccagggta ggaacgtcat cgtggcagca ggtaatgatt
     5761 tggtgccggt acggatcctt cagttgggac cgggggattt ttgccgtctc gcatttcaag
     5821 taacgtcggc acgatctccg tatgagattc tttacggcgg gccggctttg cctgatgacc
     5881 gtcggccaac ttggacaagt gaggatggac tcttgttgga agtgcgcgaa taccgggatt
     5941 gtaacctcaa cagctttgaa tccgtgaagg ccgctttcga gtcatccagg cccctcgggg
     6001 ccgattatgt ttccggggtg ttccacgccg gtcatccgtt tcctggtccg cctgggcctt
     6061 acatgagtcg atattcggga atgttgcaca ttgcgcaaac gggagtgtat ggcttctaca
     6121 catcgagcca ggactgtagc tttctcctta tcgacggtaa agttgtggta tccgcgccgg
     6181 ggcgtcatcc gcccagctat caggcgagac ccggtcttcg gaaggacatc cagttcaccg
     6241 cgggagccca tcgttttgag tactatcatg cagccacgac cgaagtgggc attgcggtgg
     6301 cggcttggga ggttaacccc aaaggtgatc agccggcacc cgcggtcatc cccggtgagg
     6361 cctttcgggc caatctcatt atgcgtggcc gggcaggacc cccgatgagt cccgcgcagc
     6421 ggtttctccc ggatttcgaa tttgtcattg tcggtgaagt tcccttgcct gatcgagatt
     6481 tgccgttgct agccgtccgg ttcacagaca aatcgccacc gcctttggta tccagtgcca
     6541 agatcgaatg ggattttggt gacgggcaaa cctcccaggg acttgccgtt gatcatgtct
     6601 tcttgcgacc aggtgttttc acggtcaagg aaacggtaac gcgggcagga cgaccgtttg
     6661 agattgcgca aagagtgtgg attggctccc cgcggactca gcaaaaggga aaacccttgc
     6721 agttggatga cctacttccg gtattggaga catatcagcc ggagaaactc gacgcccaat
     6781 ctttactcca gtttgtgacg gcttttctga ctaaagccga tcagtatttg ccgcccccgc
     6841 cacctaacac cgtgttcgtg gatgaagaag aggcaaaggc gagctcggcg gcagataaac
     6901 tagacgataa gacgcgagag gccgagcgtc gcaaatatct gcagaaggcc ctcgatgctg
     6961 cggtgttagg gttagaaaaa ggccagcaaa aaggtgatca agatatcctt gcgattgttc
     7021 ggaatgttgc acccattgcg aggaattatt tcgccgattc gaagaaggca ctcggactgt
     7081 ggacgacggc aagtgaaagg cttgaaacga gtgaagccaa actcgaagcc tatctgaaca
     7141 ttgctgatat ctgcctcaac gacttggttg atgttgccac ggcgaaagat tggctcgata
     7201 aggccgcgac catggttggc ggccagggac ggcaggacgc tttagctgcc ttgtaccaac
     7261 gacttatggg cgactattgg gcggccaagg gtgatcaaga ggctgccatc aaggcctatc
     7321 gggaggcgga gcggttggtt gcggatcgtc ggaccgaaat cgagcgaacg gcttggcggg
     7381 gggcacatag ccggtcggta gagcagtttc tgaaatcggg agagttggat cgtgcggctg
     7441 ccgaacttcg cctctggttg gatgaatttc caggcgatac gatcgagggt tacttacccc
     7501 tcctgtcggg aaaatactgg tttgcacgcc aacggtatga ggttgtttgc gccctcgcgg
     7561 accggatgtt aattgtcaat ccgtattcgc catacatcga agagttgtta ctcctttcgg
     7621 cgaggagcga cgtagcccgc ggtcaaccgg agagggccaa agcgacgctg gaatcccttt
     7681 tgaaggatta tcccggtagt ccattcgttg gtgaggccaa ggagctgctt cagaaactgg
     7741 gtacaggtaa gccgtgatcg ccacggaggc atgattttgg acagcgctgg ggagcaggaa
     7801 atgctttgtc gaactgatcg actcgcttgg gtaacgatag tgtcggtgtg cttggcgatt
     7861 gggggaagat caggcctttc actatcggca gaggtcacat tgcgcattcg aaatgccccg
     7921 ccgctgcaac ttgttgggct tatccagcgt tgggaccgcg acgggaaccc tgtccggccg
     7981 gtggacccaa aggccaagat cgatagccct cagtgtatct cgggccgtca gcggaaccag
     8041 gaaacttggg tctttgaggg cctttcgccg ggcaggtacg atgcggtcct tttgggaaat
     8101 gagcggatcc gcattgatgg tttcgacttc ccgccggtgc tcgagttcga cccgtttttc
     8161 cgaggggata ccgaagtgga cgaggagcac cgaaaaacga tcgaggagga catccgggcc
     8221 tcgcgtcatt atgagaataa ggtcgagccg ctgtatttcg gtggcaatga cgaggtggtg
     8281 cgcgtcctcg tcatgttaat tcgggacctt cccaccagct acgaggccga ttttccgggg
     8341 gctgccacca tccgtcacga aatttggcaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnggccgcgg tggccattga gcctccgatg gcggcgaaac tctttcccgc
     8521 aaaaaccgcc gttttctttt ccatacctga cgtgcctgag ttgaaaaggg cggtcctcgc
     8581 gtcttcgtcc gggcagatgt tacaggatcc acagttgcga cccttcttga cggatttgtg
     8641 gggcgggatt gtcgaggccg ccgcagtggt cgagaaggag actggcgtct ccttggaggc
     8701 gatactcgca attcccaagg gtgaattatc cgtgggcttg gtatcgtctg aaaagcaagg
     8761 ctgggttgtg ttaatcttta tggatgccaa ggagaacatt gaaacgctca agaagctcat
     8821 cgaccgcggg cgagaggccg cagtgaaggc cggagccatt ctcaaggagg aggacgtcaa
     8881 tggtttgccc ctcacggtgc tccggccgag agagcgccaa ggaaatgagg ataatcgacg
     8941 gccgcagccg cttgtggcct acgccattaa cggggcaacg ctccttgcag ggctagcagt
     9001 tctgggggag gaggatttgg acgtcatccg tgacgttgtt gggagacttt ccggaacgtc
     9061 ttccagtaca gacactttga gcgggcagag caagttcaat caatttctct cgagcataag
     9121 acatgccacc cgaggtaaga ttcacacact actatttgct gatcccatcg aggtcctgcg
     9181 gggatacgcc aagatcgaaa cgggggctgc tgtcgcgctg gcgattctgc cggtgttggg
     9241 attggatggg ctgaattcgc ttggcggcgc aggcagcgca gaaatatccg gctgggacta
     9301 cgtgcgggag ttccacgtgt ttttggacaa tccccggcgt ggggctctcg acgtaccggt
     9361 cttcaaacct gccaatctcg agcctgaaga gtttatccct tccagtgtta tcacctatgc
     9421 gacggtgtcg ctggacttac caacaacggt ttccagattg gccaaacttt atgacggttt
     9481 ccagggagat ggggcctttg agcgtaggat tggtgggcga accgaacgtt tcctgggggt
     9541 taaccttacc caagaattcc tgccagccac aacgggtagg attagccttg ttcgttgggt
     9601 ggaacagccg gtgacggtga ccagcatttc caatggattg atcgtggagc tcaaggatga
     9661 ggacgtggcg gcaatgatcg tggatcgcat ctaccagacg cgacaagcca atttcgaaaa
     9721 aaaggagttt ggcacgtggc aatactatca atccacccgc acaagaccgg cctccctacc
     9781 cgcaacggtg aggtttcctg ttccgtgttt ggcagcgctc ggtcgcttct tgatcgtgac
     9841 agatagcccg gcgctcatgc aagagttatt cgcaacaacc agtgatccgc cgcgggcctt
     9901 ggtggagtcc ctggaattca aggttgttcg aggccggatc aaagaccttg ccggtcggga
     9961 tgaagtttcg atgctcgtgt tccagcggcc tgatgaagcg gtgcgcgtct tttacgaggg
    10021 agtggtcaat caacccatca cggaggcaat gcgaacctcg gctgagtcga atccgctttt
    10081 gaaagccttc atcgcggccc gagagcggaa tgcccttcct ccatttgagg tcttgcagcg
    10141 ctactacgct ccacaggggg ctctcctaat cgatgatgcc accgggttgc acctgatcga
    10201 cttcggattg cgtaagaagc tcccgtgatg gtgccttcga acggcggttt cgttgctcaa
    10261 gatagacagc aagcacggcc aaatcggcag gggtgattcc actaattcgg ccggcttggg
    10321 cgaaatcgac agggcgaatc ttcgagagtt tttcgcgtgc ctccgcgcga agatggggaa
    10381 cgtcgtcata attgatatcg gaaggaatcc gcttttcggc cagacggcgc tgccgttcga
    10441 tagcggcttc ctcccgtgct aggtaacctt cgtatttgac gtcatacata acctgctcga
    10501 cgacgtcttg tctaaatgcc gctagttcgg gacagatttg aacgagttcg tcccacgttg
    10561 tttcgggctg ggccagtcgc ttgatgagag gcacgccgtg atatttcacc gattccaaga
    10621 tagcccgcag gcgatggata ccatcgagtt tttcctgaaa ccgcctccat cgctcgtcgc
    10681 tcacaagtcc caactcccgt cctaatggcg tcaaacggcg atcggcattg tcatgacgca
    10741 atcgcagtcg atactcggct cgacttgtga acatccgata aggtt
//
LOCUS       DAOSUT010000139        10721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3438_length_10721_cov_10.392181, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000139 DAOSUT010000000
VERSION     DAOSUT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3438_length_10721_cov_10.392181"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..682)
                     /locus_tag="PLD05_12030"
     CDS             complement(209..682)
                     /locus_tag="PLD05_12030"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOP78214.1"
                     /translation="MSDSPDSRFADGQTEPGGQRNQQQRIIVDEDWKAQVEREKREAA
                     EKKDSQAGLDPPLPPPTLTALSSSLAMQAMAALGLLPDPLSGKLEVRLNRARHLIDTV
                     NLLLEKTKGNISEEESRAMEEMLHELRLTYVQVQSRLASQPTSEKEKSPSEGGSS"
     gene            1293..2486
                     /locus_tag="PLD05_12035"
     CDS             1293..2486
                     /locus_tag="PLD05_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HOP78215.1"
                     /translation="MSVGEWVSSKVFTLVHRHNLVYNTCWEDPRLDRVALNIGPEDTI
                     LVITSAGCNALDYALLGPKHIYAVDMNPRQNALLELKMAGIRRLDFPTFFQIFGEGRY
                     RGIEEVYRSSLRRELSPFARRYWDKHIKRFFGRKRSFYFRGTAGTFARLMNTYITKVA
                     RVRPWVEKILNAESLSEQREIYEKHLKEQVWTPLLRFLLSRDAMLSLVGVPRAQRQQI
                     ESQYEGGVAQYVQDCVDAVFGKLPLRDNYFWRVYITGSYTRECCPEYLKEENFYLLKN
                     GLVDRISTHTNTVEGFLVENDVTISRYVLLDHMDWLSGRFFPLLEAEWQAIFDRAAPG
                     ARAIWRSGGLRTDFVDRVRVQLGGRTVALGEQLTYHRELAAELHAKCRVHTYGSFYIA
                     DLNVP"
     gene            2552..3310
                     /locus_tag="PLD05_12040"
     CDS             2552..3310
                     /locus_tag="PLD05_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOP78216.1"
                     /translation="MGLWTDLKILYRMVFHPGRGQDHASRMESFYAPQAAYYDSFRRR
                     LLKGRDDLWQAIGVPENGLWVDLGAGTGANAEYFGDNISRLRKVILVDLSPSLLAVAQ
                     ERIKSHGWTNVETQVADATVFQPPEPADVVTFSYSLTMIPDWFAAIDNAWRMLKPGGK
                     IGVVDFYVSRKHPAPGMKRHGACARLFWTFWFNYDDVFPSPDHIPFLQRRFRQILLEE
                     RRAKVPYLPLARAPYYLFVGEKTEESPAPPYFSA"
     gene            3448..4206
                     /locus_tag="PLD05_12045"
     CDS             3448..4206
                     /locus_tag="PLD05_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOP78217.1"
                     /translation="MSHQINRVALTLMAHPDDAEFCCGGTLARLRELGWEVHIASLAP
                     GDCGTMVENRWDISSRRTREAAAAARVIGATYHCLDERDGFIVYDKPTIQKVVDLFRR
                     IAPGLVFTHALRDYMMDHEMAAILARAASFLHGAPNASAFPLKENTGVPYLYYCDPPE
                     GLDPYGHAIEPTTYVDISAYLDKKAEMLACHESQREWLREYHGMDEYLESMRRLARWR
                     GQQIGVAAAEAFVQHRGHAYPRNDLLAELFPRGS"
     gene            4341..4643
                     /locus_tag="PLD05_12050"
     CDS             4341..4643
                     /locus_tag="PLD05_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOP78218.1"
                     /translation="MAKKQSKAKATSGAKRPLTAREKQVVRLISLGCSVKEAAAILKL
                     APSTVDNHKANAMKKLGVNKLPLVTRMAIKLRISPLNDRLTPLEKKRSGRKADGWN"
     gene            complement(4686..6182)
                     /locus_tag="PLD05_12055"
     CDS             complement(4686..6182)
                     /locus_tag="PLD05_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78219.1"
                     /translation="MTAWLDNERLSAYLDGELSPQERQIVEKLLASDSAARQLVEELR
                     AQRELIRQLPRFPIPRDLSRSVIALAEERRAKRQGSGQAERTPVVPSAESSDRSPVRF
                     LKRLIRPRNLGWALVAASIGIAIALISPQLERRGDQVEVARRDNVQKDLAAEKQREPG
                     VGEFRAAPSGRLRGMPESPSRESIGRPRMAGPAESALEADATAIGSGTAQADRQQAPA
                     EEAPQVIIERMVPMLGYQSAAREMGGMSPVDSRVAPYEIVCEVTEKASLQTLVGRVIR
                     KHQAIPEEAALDAAWEFAANREDHREQQGTGGGGDRQDARPDVQVAFRESEEFEEAVI
                     EFSATLAQVQGVLAELGAQPGQIRNIMIPQELSGILEEGRYPGSDRIAAKVGRPEGFG
                     TAGARALPSMPKAAAPSPPLAAGAASQAKPVDEGQAAIGEVRRQEAGAAMSMRRSVTP
                     PTSGPGDLYRIRVVLRHKRASETNPSPATVPQTQGLGGAVRESPSPQK"
     gene            complement(6596..7156)
                     /locus_tag="PLD05_12060"
     CDS             complement(6596..7156)
                     /locus_tag="PLD05_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP78220.1"
                     /translation="MKEDSALIQEVLCGSPVAFGRLVEKYQDRLYDTMLHFVGDADET
                     MDVVQEAFIHAYLNLKSFQGESAFYTWLYRIAFNIAATYHRRRKPVQSMDRLYAETGH
                     ESADPGCSPVLQVEQEERARKVRQAIATLSEDHRAVLILRDLDGYSYEEIAQILAIPI
                     GTVRSRLHRARLELRERLKSSEYDLL"
     gene            complement(7325..8689)
                     /locus_tag="PLD05_12065"
     CDS             complement(7325..8689)
                     /locus_tag="PLD05_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP78221.1"
                     /translation="MIEFAIALACAGCLAAVETFLVGVQAWEHRRFAASRLRSSPKAQ
                     MQAAGRAMVIIPCRGIEPNLKGNLERFFRQDYSNYGLRFVVESHDDPAAEVIQQLIEE
                     YPNVPAELVTAGLANGEAQKIHNLRVATEKIPPEVEYLVFADSDAAPSEHWLTAMLMR
                     LGDPKVSAVTGYRWFIPKTGAFHEAVVCALNGAYAMLMSRNTPNLVWGGSWAIRKEVF
                     EELDIRGAWRGRICDDLVAADRLLKCGRHVEYEPACLVATSCQPDLKAVFEFASRQFF
                     LLRRVLPLWWFTDLVVGYLGTVVFWLAIVTLIMVPDTLVRWTSAIVAAVSYALHWARA
                     ELRVAAVRTYFPRLAGEASFRRVFWYDRVLSPAIQFIGLLANLNGGLRRQIIWRGIIY
                     RMGKKAQIEIVRPSNGSSAYSEINGGGPSAAAAPILGGPEDTKCQEAPEMRRPEKKVH
                     PVAA"
     gene            9949..10500
                     /locus_tag="PLD05_12070"
     CDS             9949..10500
                     /locus_tag="PLD05_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78222.1"
                     /translation="MSKTKRLHVGSFRFNPPPEPYPDLDLKMVLRCNPYLEYQYRAVG
                     FGNTFEEALENLFEKIRISGFVVDRRTRYRITNALSYGGWETSCRLPSAKPQEDELAT
                     AIGTLLVAMVEQPEDVPTPQVLYSISIAWNALEDEKQFTRPQGAKRLFGGFPIAKWLR
                     KATDWLAWPIRALLNISKLSWNT"
BASE COUNT         2433 a         3092 c         2798 g         2398 t
ORIGIN      
        1 cggttttctc aatcgattcc gacgttcgct tgccctcacc ccgaccctct cccggaggga
       61 gagggggacc ttttggaata ggctgttaca aggcggctat taggaacgcc aagtgaagcc
      121 gcaattacta attcaagccg tgccaatcgg cctttaaatt catgcggagt taaagagact
      181 tcaatatgaa cgcgggggct ggtcgaggtc aggacgaccc accttcgctc ggcgatttct
      241 ccttttcaga agtgggctgg cttgctaacc gagactggac ctgcacatag gtgagccgca
      301 actcgtggag catctcctcc atggcccgcg actcctcctc cgaaatgtta cccttcgtct
      361 tttccaagag gaggttgacc gtatcaatca ggtgtcgcgc ccgattaagt cgcacttcca
      421 gcttaccgct gaggggatct ggaaggagcc ccaaagccgc catggcctgc atggcgaggc
      481 tgctgctgag tgcggtcaat gttggcggcg gcaatggcgg atcaaggccg gcttgagaat
      541 cctttttctc agccgcttcc cgcttttctc gctcaacctg ggctttccag tcctcatcca
      601 caataatccg ctgctgttga tttcgctggc cgcccggctc tgtttgacca tccgcaaagc
      661 gactgtccgg tgagtcgctc atgtacaatc ctccagatat cggaaacctc acgagttagc
      721 caagagcaca cgtaagttcc ccggagattc tttatcagtt tggcggtttg agcgggcaag
      781 tcaagaatgg ccgacaaaaa accaaaaaac ccttcgagat tgagcccttt gcgggaaatt
      841 tcttcccatt ggaaatagtg aaaacggcca ccacagcgcg tggccctgca tgagcgaggc
      901 cacaacggca agtgcccctc cgtcacatcg gacaggcaca agtccagccc atagcggtgg
      961 tgctgagcca ttggaatgct gcgctaggag caacgtttgt cgccgggctt gttacgtctc
     1021 agagtcacgg gcgacatccg atctgcgcgg gtgtcttttt cctcacgaga attaggaagg
     1081 ccaagtggct tcgctgctgg cggtggcgaa aatcccctcc aagcttgtat ttcgccagat
     1141 ttgccgtata ttgggggtta tgttaccaaa tcgtgggggg aggattggtt ccacagaacg
     1201 ccggtttccg agtgcgggga aagcgaaaga gactgcgggt tcctccatcg ggaaacggca
     1261 cacctcatca atagagacga ggagccgcga gcatgtcggt cggagaatgg gtcagcagta
     1321 aggtcttcac tctcgttcat cgccataatc tggtttataa cacatgctgg gaagaccctc
     1381 gtctggatcg ggtggcgctg aatatcggac ccgaggatac catcttggtc atcacttcgg
     1441 caggctgcaa cgccttagac tacgccctcc ttgggcccaa gcatatttac gcggtggata
     1501 tgaatccccg gcagaatgcc ctgttggaac taaaaatggc gggcattcgc cgcttggatt
     1561 ttccaacttt cttccagatt ttcggtgaag gtcgttatcg cggcatcgag gaagtctatc
     1621 gttcgtcgtt gcggcgtgaa ctcagcccct ttgctcgccg ctattgggac aagcacatca
     1681 agcgattctt cggtcgtaag cgatcgtttt acttccgggg aacggcaggc acttttgccc
     1741 ggctcatgaa tacgtacatc actaaggtgg ctcgagtccg cccatgggtg gagaagattc
     1801 tcaacgccga atctcttagt gaacagcgag aaatttacga aaagcacctc aaggaacagg
     1861 tctggactcc cttgctgcgg tttctcctca gtcgggacgc gatgctttcg ctggtgggcg
     1921 tcccgcgagc ccagcgacaa caaatcgagt cccagtacga aggaggtgtt gcccaatacg
     1981 tgcaagactg cgtggatgcc gtcttcggta agctcccgct gcgagacaac tatttttggc
     2041 gtgtgtacat cacagggagt tatacccggg aatgctgtcc cgaatacctc aaggaagaga
     2101 acttctacct gctaaagaat ggtttggtcg atcgtatctc gactcacacc aacacagtgg
     2161 aaggtttcct cgtggagaac gacgtaacca tcagccgcta cgtcctcctc gaccacatgg
     2221 attggctgtc gggccgtttc ttcccgcttc tggaagccga atggcaggcc atttttgatc
     2281 gggctgcgcc gggcgcccgc gcaatctggc ggagcggtgg tttgcggaca gactttgtcg
     2341 atcgcgttcg ggttcagttg ggcgggcgta cggtcgcgtt aggtgagcaa ttgacgtacc
     2401 atcgggaact cgccgccgaa ttacacgcca agtgccgcgt tcacacttat ggaagtttct
     2461 acatcgccga tttgaacgtc ccctaaacgc gcgatcgtca gcaacagact aagggctgcg
     2521 tctgagctac tcaaggaggt tcattcatcc catgggtctt tggaccgatc tgaaaatcct
     2581 ctatcgcatg gtctttcatc cagggcgtgg gcaggatcat gcctcgcgga tggaaagttt
     2641 ttacgcccct caagcggcct actacgacag ctttcgacgg cgactgctca agggtcggga
     2701 cgacctttgg caggccattg gcgtaccgga gaacggacta tgggtggatt tgggggccgg
     2761 caccggggct aatgcagagt attttggcga caacatttct cgtttgcgga aggtcatcct
     2821 ggtcgatctc tcgccgtcgc tacttgccgt agcccaagag cgaattaaga gtcacggctg
     2881 gacaaatgtg gaaacccagg tggccgacgc tacggtcttt caaccaccgg agcctgccga
     2941 cgttgtcacg ttctcctatt ctttgacaat gattccggat tggtttgccg ctatcgacaa
     3001 cgcctggcgg atgctcaaac ccggggggaa gatcggcgtc gtcgattttt acgtctcccg
     3061 caagcatccc gcacctggaa tgaaacgtca cggggcatgt gcccgactgt tctggacgtt
     3121 ctggtttaac tacgacgacg tttttccatc gccggaccac atcccctttc ttcaacgacg
     3181 gttccggcag attctgcttg aggaacggcg agccaaagtc ccttatttgc cgctagctcg
     3241 ggcaccatac tatttgtttg tcggcgaaaa aaccgaggag tcgcccgcgc cgccttattt
     3301 ttctgcatag cgtggcattt tcctcaacac caactcctcc ttgtggcggg acttgccgtg
     3361 aggggtgtct ttacgctaac gtaatgactg ttttcccagt cggcttgttc tcttaatgct
     3421 gcttattggt ggattcggag gcgccatatg tcgcatcaaa tcaatcgggt ggcgttaact
     3481 ctcatggccc atcccgatga tgctgaattt tgctgcggtg gaacgttggc tcgcttgcgg
     3541 gaactcggtt gggaggtcca catcgcaagc cttgcccctg gtgattgcgg caccatggtg
     3601 gaaaaccgct gggatatttc aagccgccga acgcgagaag ctgccgccgc agcccgtgtt
     3661 atcggagcga cgtatcattg cctcgacgaa cgcgatggat tcatcgtcta tgataagccg
     3721 acaattcaaa aggtagtcga tctattccgc cgaattgccc cggggctggt attcacgcac
     3781 gccttacgcg actacatgat ggaccacgaa atggcagcga tcctcgcccg ggccgccagt
     3841 tttctccatg gagcgcccaa tgcgtccgct tttcctctca aagagaatac tggggtaccg
     3901 tacctgtatt actgtgatcc gccagagggt ttggacccat atgggcacgc cattgaaccg
     3961 acaacgtacg ttgacatttc ggcatatctc gataagaagg cggaaatgct cgcctgtcat
     4021 gaaagtcaaa gggagtggct ccgcgaatat cacgggatgg atgaatacct tgagtccatg
     4081 cgccgcttgg cccgatggcg gggacagcaa ataggggtag ccgccgctga agcattcgtt
     4141 cagcatcgag gtcacgcata ccccagaaat gatctcctcg cggagctttt cccacgggga
     4201 agctaggact tggaaaacga tttggccgct cgtgtgacat aacaaaagaa ttgataacag
     4261 aggcaaacag cctcctgtgg ttcgtatcga agtttcacaa tcgttgggtg ggttcctgag
     4321 agagtggtga ggtgatggcc atggcgaaaa aacaatcgaa agcaaaagcg accagtgggg
     4381 caaaacggcc gctcaccgcg agagaaaaac aagtcgttcg cctgattagc ttggggtgtt
     4441 ccgtcaagga agcagccgct atcttgaaat tggctccgag cacagttgac aatcacaaag
     4501 ccaacgccat gaaaaagctc ggcgtcaaca aattgccgct ggtaacccgg atggccatca
     4561 aattgcgcat cagtcccctc aatgaccgtc tgacaccgct cgagaaaaag cggagtggac
     4621 ggaaagcgga cgggtggaac taacctggtc acctcattct cgatcagtga acggctaact
     4681 cgtgctcact tttgagggga gggagactcg cgcaccgcac cccccaagcc ttgcgtctgc
     4741 ggaaccgtag cgggggacgg attggtttcc gaagcacgtt tgtgacgcag aacaacccga
     4801 atgcggtaga gatctcctgg acctgaggtg ggcggcgtca cacttcgccg catgctcatt
     4861 gctgcacccg cttcttggcg ccggacttca cctatggccg cctgcccttc gtctaccggt
     4921 ttcgcctgag aggcagcccc agccgccaaa ggcggcgagg gagccgcagc ctttggcata
     4981 gaaggcaaag cacgtgcccc cgctgttcca aagccttcag ggcgtcccac ttttgccgca
     5041 attcgatccg aacccggata acggccttct tcaaggatcc cggacaactc ctgaggaatc
     5101 atgatgttgc ggatctggcc gggctgcgca cccagctccg caagaacacc ttggacctga
     5161 gccaatgtgg ccgaaaactc gatgacggcc tcttcaaact cctcgctctc cctaaatgcg
     5221 acttggacgt ctggtcgggc atcttgccgg tcaccgccgc cacctgtacc ctgctgttcg
     5281 cggtgatctt cccgattggc agcaaattcc caagctgcat ccagcgccgc ctcttccggg
     5341 attgcttgat gcttgcgtat gacacgcccg acaagcgtct gtaatgacgc cttctcggtg
     5401 acctcgcaga caatctcata cggcgccact cgggaatcga caggcgacat gccacccatt
     5461 tcgcgtgctg ccgattgata gcccagcatc ggtaccattc gttcaataat gacctgtggt
     5521 gcctcctcag caggggcttg ctggcggtca gcctgggccg ttccgctacc tatcgccgtc
     5581 gcatccgcct ccagggctga ctcggcagga cccgccatcc taggacgccc tattgactct
     5641 cgtgaaggag attccggcat tcccctcaaa cgtcctgacg gggccgcacg gaactcgcca
     5701 actcccggtt ccctctgctt ttcagccgcc aaatccttct gcacattatc ccgccgggcc
     5761 acctccacct gatcgccccg ccgttccaat tgcggcgaaa tcaaggcaat agcaattcca
     5821 atggaagccg caaccagggc ccaaccgaga ttcctaggcc gaatcaatcg tttcaaaaac
     5881 ctcaccggcg accggtcact cgattcagcc gacggcacca cgggtgttcg ctcagcttgg
     5941 cccgaaccct gtcgcttggc gcgacgctcc tcagctaggg caatcaccga ccggctcaaa
     6001 tcccgcggga taggaaagcg agggagctgc cgaatcaatt ctcgctgagc gcggagttcc
     6061 tccaccaact gcctggcagc cgaatcgctt gccagaagtt tctcgacaat ttgcctttcc
     6121 tggggggata attccccgtc cagataggca ctcagtcgtt cattgtcgag ccaagccgtc
     6181 atcggaggta ttcctaatct ttctgcatag cgctcgcacg gacgattttc ccgcaaatgt
     6241 ttttcgggcc ctcacaaaaa agcatcgtca taatgtcctc gcagacctat cggaggaatg
     6301 cccagaggaa cccgctgtat acgacggttt ttcttcgctt ttgaccaaat aacccgaaac
     6361 ccggttattt gacgtcctca tgccataatg aagttccaca taaatttcgg aaaaaacttc
     6421 tcaacttttg atgtcccgat tcaaacgcgt ggaaatccaa tcggtaacgg gcatccgtta
     6481 atggttgtcc acggaaacct aacggattct tgccgcccgc tgctggagcc ggcacaaaat
     6541 cgatgtctaa gggatacaat caatcgccag acaccatgaa tgatcagtaa ctccatcata
     6601 ataagtcata ttctgagctt ttcaatcttt ctcgcaattc caacctggcc cggtgaagtc
     6661 ggctgcggac ggtgccaatt gggatggcca aaatctgggc aatctcttcg tagctatagc
     6721 cgtccaggtc acgcaagatg agaacagcgc ggtggtcttc gctcagcgta gcgatggcct
     6781 ggcggacctt tcgtgctcgt tcttcttgct caacctgaag caccggcgag cacccgggat
     6841 cggcactttc gtggcctgtt tcggcgtaca gccgatccat ggactggacc ggttttctcc
     6901 ttcggtgata cgtcgcggca atattgaagg cgatccgata aagccaggta taaaacgcgc
     6961 tctcaccctg aaacgacttc aggttcaaat aggcgtggat aaacgcctct tgaaccacat
     7021 ccatggtttc atctgcgtcg cctacaaaat gaagcatggt gtcgtacaat cggtcctggt
     7081 acttttcgac cagccttccg aatgcaacgg gcgaaccgca gagaacctct tgtatgagag
     7141 ccgaatcctc tttcactgtt tgctcaaccg ctcaccaccg taagccctgc cgtcaacgcc
     7201 gcaattgccc aacttctcgt gagactctgc agcccctgtc acccctcaag tctcatcatg
     7261 aggttatcag ggcatattgt caaacggtgg tctactgcca ctccgattcg ctttcgccaa
     7321 tagactacgc ggcaactgga tggacttttt tctctgggcg tctcatttcc ggtgcttctt
     7381 ggcacttcgt atcttctggc cctccgagga ttggggctgc cgccgcgctt ggaccgccac
     7441 cgtttatctc cgagtacgct gaggagccgt tgctgggtcg gacaatttcg atttgggcct
     7501 ttttccccat tcggtaaatg atgccgcgcc aaataatctg acgtcgcaag cctccattca
     7561 ggttcgctag gagacctatg aattgaatgg cgggactgag cactcgatcg taccaaaaaa
     7621 cccgtcgaaa acttgcctca ccggccaaac ggggaaaata agttctcact gcagccaccc
     7681 gcagctccgc ccgcgcccag tgaagcgcat acgatacggc agccacaatc gccgaggtcc
     7741 atcggaccaa agtgtcaggt accatgatga gtgtgacaat cgccagccaa aacacaaccg
     7801 tgcccagata cccgaccact aggtccgtga accaccaaag ggggagaacc cgtcgcaaga
     7861 gaaagaactg ccgggaggca aattcaaaca cggccttgag gtccggctgg cacgatgtgg
     7921 caaccagaca tgccggctcg tattccacat gccggccaca tttgaggagc cgatctgcag
     7981 ccaccaggtc gtcacagatc ctgccccgcc atgccccccg gatgtcaagc tcctcaaaaa
     8041 cttcttttcg gattgcccaa gagcctcccc agacgagatt cggggtattc cggctcatga
     8101 gcatcgcata tgccccgttc aaggcacaca caacggcttc gtggaaggcc ccagtcttgg
     8161 gaataaacca ccggtagccc gtcaccgcgg agacctttgg gtcgcccagc cgcatcagca
     8221 tagcggtcaa ccagtgctcc gacggggctg cgtctgaatc agcgaaaaca aggtactcta
     8281 cttccggagg aatcttctcc gttgccacac gaagattgtg gatcttttgc gcctcaccgt
     8341 tggccaaacc ggctgtaaca agctcggcag gcacattcgg gtactcttcg attaactgct
     8401 gaatgacctc agcggcaggg tcatcgtgac tttccacgac aaaccgcagg ccgtagttgc
     8461 tgtaatcttg ccggaaaaat ctttcaaggt tgcctttcag gttcggctcg attccccggc
     8521 acgggataat gaccatcgcg cggccagcgg cctgcatctg agccttgggc gaggaccgca
     8581 atcgactggc ggcgaaccgg cgatgctccc acgcctggac cccgacgaga aaagtctcaa
     8641 ccgcggcaag gcatcccgca caagccaaag cgatagcaaa ctcaatcatt gaggggaatc
     8701 cttcccgctg gaggaacaag agaggcatcc attgcgacag aaagtttcac gttgggactc
     8761 aaaccatctt gccccgcgaa gccctcgaaa taaccttacg ctcgcgcaaa ggccaccgaa
     8821 ccagccaaat tattcatgtt acccgttcgc gcatacattc ccccttcgcg tcaaaatagc
     8881 ccgtttagct cgtccggtca atggcaaata ttgccttcgc atacaaacga caccatgatg
     8941 ccgtgtgcat caatcttgta gctagacatt tcgtgggcgg agtttacaga aggcgtctca
     9001 gcagcaaggc acaccgaaca caaggccacg caactcgcac ctggcgttgg ccgcaagttc
     9061 accccagaac atcgtgcaaa tgaagaaagt actccagaat tggaagggcg acgtcgcgca
     9121 agtggacaac ccgcaacgag atagactgcc ataaaagatg ccaataagaa aaaatcgcaa
     9181 cgtatccgcc ggcaacagaa aacgcgctac acacaaggcc tccttttctg gcgggactaa
     9241 cgcgctgccg ttaacactct gaactgcaat agcccggaga gcacgcaacg ccgagatgtc
     9301 gaacccagga tcgcgcggtt cacctcttcc ccaaaaagca gtgctggatt gctcagaaac
     9361 tgtcagtggc gtccaccaag tcggcaaaga gcctcctgag cagggtgcaa ggcctcgtat
     9421 ccgggtttgt cctagacagc ctgtgccaca gggtgaagga ttgccgcaag ggaacttgcc
     9481 tggatttgcc aaattgttcg tccgaagggg gcttgatgcc cgttgatttc cgcataaaat
     9541 aactcgtgat gggtgtcaga tgaatgtaaa taggcgcatc caaaggacag tcgcatagcg
     9601 aaagattgtt agtatcattg attctgaccg gccgggaatc aggcaatttc agaagagaga
     9661 aggcctcatc ccggctgatg taaactatca aaagagatat acagagatat atcctttcat
     9721 atgtaaacct cgcgtctatc gcatcctatt ctgtctcttc atatctcagc cctcctatta
     9781 gctcgtaaat gttatcgatg tgatttaggc tgtactccct tatctcaagc ttaatcactc
     9841 tggttcttgt attcccgcga tctcggcgtt gtatcctgcg ccggtatatc tttcaccggc
     9901 ttctggtcga tcttttgtga caacaaacac ccagacaagg agattctcat gtcgaaaacc
     9961 aagcggcttc acgttggctc gtttcggttc aatcctcctc ccgagccata tccagactta
    10021 gatctcaaga tggtcctgcg ctgtaaccct tacttggagt atcagtaccg cgcggtcggg
    10081 tttggcaaca cgtttgagga ggcactggaa aatctgttcg aaaaaattcg gatatccggg
    10141 ttcgtagtcg atcgcagaac aaggtaccgc attactaacg cattatcgta cggcggttgg
    10201 gaaacctctt gtcggttgcc ctcggccaaa ccgcaggagg atgaactcgc cactgcaata
    10261 ggcacactgc tcgtagcgat ggtagaacag cctgaggacg tcccaacacc gcaggtactt
    10321 tattccatct caatcgcctg gaatgccctg gaggatgaga agcagtttac cagaccccaa
    10381 ggagctaaac gcctgttcgg tggattccct atcgcgaagt ggctacgcaa ggccaccgac
    10441 tggctagcgt ggccaattcg tgctctatta aacatttcga aactttcatg gaatacatag
    10501 acacattact gaggagaagg gtcaggagta gtttagtctc agagcacttt cgcctggctg
    10561 acggtccgct tggaacgacc ggcaccaggc ggaatgtagc tcggagaacc ccgttttgat
    10621 catcccgctg gatgccaacc cgggttaatc caccaccctg gccctctcct ttgtctaatc
    10681 tttctcatta gcgtttatcc caaaaagtcc ccctctccct c
//
LOCUS       DAOSUT010000140        10713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3441_length_10713_cov_8.760555, whole genome shotgun sequence.
ACCESSION   DAOSUT010000140 DAOSUT010000000
VERSION     DAOSUT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3441_length_10713_cov_8.760555"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..>1106
                     /locus_tag="PLD05_12075"
     CDS             476..>1106
                     /locus_tag="PLD05_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78223.1"
                     /translation="MSDAQTPQTFDPYYTWFGIPPTERPISYYRLLGLSPLESNEAAI
                     EAAADQRLAYLRTKTLGAHAMFAERLISEVIQARLVLLDPVKKQAYDAALSRSLQERP
                     VISQEQTPDSQLRQPQVAVTLGMESYTPGDTHRFRKKSRFSKEQWIKALALSISFCFV
                     LVAVGLWFSYKKIGRQQDPNSSPKQEELAGGPAAPRANEGSSKEVPEQIQ"
     assembly_gap    1107..1206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1207..3046
                     /locus_tag="PLD05_12080"
     CDS             <1207..3046
                     /locus_tag="PLD05_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78224.1"
                     /translation="PMEPSKTPEELAAEAALKKLQELEAHYAEATRSAEARAAAWDFR
                     GAAAELSQLHFEEASLASRVQQRLEELERMQGLKEKLITAINRAKPPLQKSQLGLKGL
                     GGEVIGADEEGLTTRLLNGKTERLEWSSLGASGVERLFSPKQFEALVGRDGEAWLAAG
                     LVALTLGNAAEAERCMFRAKELGAAIEPYLSSLAGAAFGRITEGLKAAQGALAVARQP
                     GEPGSAKERFAKVERQFRQVASQLEELETRYRSAPWYSAHQQAVAGARQAVQRGLDEA
                     AAEQLYAEAAELFAQREYFDLKPIVEQLKSQYAATAPVRDTGRKPSFTELEQAVADLG
                     RWIVVRQDGKGDFTSIQAAIDAAPVNSVIEIQDNVPYSEKLIIARRLEIRGSRKSWPI
                     LLSQGRSTPEGSMVDIRAEGVMVRRIAIVTYDPSEHTPACVYFRTPASIKSLILSGAN
                     VPALRGEGSVTGQIIDSLILSSFRALWWKASLRNCLVLNGMDLLRRCEVEGCVVQGVI
                     HLGGREGHLIRDTIVLEVTIDAGIPYQIENCAFVKPGIPGGAKNSFVANPRFRDPAIL
                     DYRLIPDSPCIGKASDGGDIGCRYTPEMIEMCKVALELRARGIIKF"
     gene            complement(3292..3588)
                     /locus_tag="PLD05_12085"
     CDS             complement(3292..3588)
                     /locus_tag="PLD05_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOP78225.1"
                     /translation="MSFYSPFDEDVVVREGHLDEYPLSPAQAEAERLATQILRAVEAA
                     TAGAVADLRVTIDGDCVILSGRCPSYHIKQKAQHAAMPFAPGILQNDICVTWRR"
     gene            complement(3711..3959)
                     /locus_tag="PLD05_12090"
     CDS             complement(3711..3959)
                     /locus_tag="PLD05_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOP78226.1"
                     /translation="MVVDVLSGNLQARAESALRASPIHELRDVNVEQQGDTIILTGVV
                     SSYYYKQLAQEAVRSVLRNHNVEVLNQIEVRPYSFDRD"
     gene            complement(4440..5114)
                     /gene="cysC"
                     /locus_tag="PLD05_12095"
     CDS             complement(4440..5114)
                     /gene="cysC"
                     /locus_tag="PLD05_12095"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOP78227.1"
                     /translation="MSGCDPKVFWHEHAVSREERERLNGHKGCVLWFTGLSGSGKSTL
                     ANLVDHRLHAMGVHSFVLDGDNIRHGLNASPAMLKERHGEEFAKRFGLGFSAQDREEN
                     IRRIGAVAKLFCEAGIIAITAFISPYRIDRDRVRATMKPGDFIEIFVDAPLEVCEARD
                     PKGLYKKARAGELKGFTGIDDPYEPPLRPEIHLDAANHSPEELADVVINYLREKGIIG
                     ATPGDR"
     gene            complement(5268..6353)
                     /locus_tag="PLD05_12100"
     CDS             complement(5268..6353)
                     /locus_tag="PLD05_12100"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOP78228.1"
                     /translation="MSGINPLRVAVIGGDGTGPEVIAEAVKVLKAVAQMEGITLQLQE
                     FDLGGERYLRTKEILPENVFEQLKTFPAILLGAIGHPDVPPGILERGLLLELRFRFDQ
                     YINLRPVKLYPGVETPLAGKGPEDIDFVVVRENTEDLYGGIGGFLKKGTPDEVAIQTA
                     IYTRKGCERCIRWAFEYTRKRNKKKRLTLVAKTNVLTYGHDLWWRTFQEVAKEYPDIQ
                     TDYNHVDACCMWMVKNPEFYDVIVTTNMFGDIITDLAAIIQGGMGVAAGANINPEPGG
                     VSMFEPMGGSAPKYTGKGMINPIAAIAAAGMLLEHTGHERAAERVHQAIQVVTGTKMK
                     SQAAGKMGYTTSQVGDLVVEALSQVNV"
     gene            6754..8283
                     /gene="lysS"
                     /locus_tag="PLD05_12105"
     CDS             6754..8283
                     /gene="lysS"
                     /locus_tag="PLD05_12105"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOP78229.1"
                     /translation="MNQDHPSSSSEQGQRPKSHLEAARREKLRRIAEMGIDPWGGRFD
                     GHRPIREIRARENEIVVVDGGDGRSAPELRGPRVRAAGRIMLLRRAGKIMFIDIRDWT
                     GKIQVLVGKAQVGERNWELAQCFDLGDIIGVDGELKRTKTGELTIFADNLHFLTKSIE
                     TPPEKWHGLADPELRNRMRYLDLAYTEGVMERFLRRTQIVHSIRATLAEEGFVEVEGP
                     TLHVIAGGAAARPFVTHHNALDMDLYLRIALELHLKRLLVGGMEKVYELGRVYRNEGI
                     SPRHNPEFTMLEAYQAYGDYHTMMDLTEKLIVQAIRATGQPLQLPWGENVIDFTPPFA
                     RKTYDELFAEHTGVNPTDPASISYFASSRGIEVVGRHPDVIKNEIFEEFVQPKLTGPI
                     FVLDYPASICPLTKRSRNNPQIAERFELFVQGMELANAYTELNDPDLQEELFSRQLEG
                     LPEEESMARMDRDFIRALRHGMPPAGGLGIGIDRLVMLLTNCQSIREVILFPLYRPEV
                     D"
     gene            8317..9843
                     /locus_tag="PLD05_12110"
     CDS             8317..9843
                     /locus_tag="PLD05_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP78230.1"
                     /translation="MYRWLLCWRYLKTRYIALASIISVTLGVATMIVVNSVMLGFTHE
                     MQNRLHGILSDLIFQSRSLEGFPDPDWHMEQIRRVAGDAIEAMTPTVVVPGMLNYQVG
                     DAWMTRPVQIIGVDERTQAQVSDFSKYLQHPENRKNLSFNLREGGYDVIDHQADGPSL
                     PRDEMALAGWEHRRRAARLRKMIEQVQQEPEQQSPSQGAEASPFTNPFERAQTNEPVA
                     AFDPETSQYTGAILGIGLASFRRNGQDHFLLLPGDDVKLTLPTVGTPPRIVSDTFTVV
                     DFYESKMSEYDANFVFVPIRVLQQLRGMIDPSTGKGYVNAISIKLKPGADMNEVRDRL
                     RNAFAPQYYAIDTWRDSQGPLLAAVNMETAILNVLLFLIICVAGFGILAIFFMIVVEK
                     TRDIGILKALGASAAGVMSIFLGYGLLLGVVGAGAGLTLGLLFVRHINEIADLLGRIT
                     GHRVFDPDIYYFYKIPTIVDPWTVGWIMAGAMLIAVLASVLPALRAARLHPVEALRYE
                     "
     gene            9836..>10713
                     /locus_tag="PLD05_12115"
     CDS             9836..>10713
                     /locus_tag="PLD05_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP78231.1"
                     /translation="MNKLTSTTDGLSSASQETGHLPRSPNGEPSDAHPLVARPHFSLR
                     KHDGSSETLRQSQDLECVDEPAEATECLLSVRGLVKSYRKANVEIPVLKGVDLDVRPG
                     EFLAIVGQSGSGKSTLLHLIGLLDHPDQGAVFLGGDRIDNVPVRIRDQLRNRTFGMVF
                     QFYHLLPELTTLENILVPVMIGRSFWSYWRQRKEYRRRAIELLELIGLGHRLKHRPHE
                     LSGGELQRAAIARALINRPQILLADEPTGNLDPETGAEILNLLRTLRDEEHLTIVMVT
                     HDHSIAALADRIVCLSG"
BASE COUNT         2303 a         2995 c         3011 g         2304 t
ORIGIN      
        1 taagctctaa ggtgtacctt ttgccgcaga gctgcgtgct cgaagccacc cgcccaagct
       61 tcacccgaaa agtgtgtagc gccgcaaaaa agcgggtaaa gtccagcgtt caacctcttg
      121 tcaaccaaaa tctgccgaat tatattcgca atcggagaat tctcttatcg agacgctcgc
      181 cgcatgccac gccctgggcg gggacttttc ggggcggcac ggggctgctg tgcggccgag
      241 gggccctacg gggagtcctg ggggatcggt catggcaccg acgctggagc ggtttgcggc
      301 cgcggtggtg gccagtggtt tgatgtcggt tagtgctgct ccattccatg ccaccctggc
      361 cgaatttcga atctgaaaat gcggcagcgg ttggacatgg tttgtccaac cccgggcgca
      421 taatgcccaa taggcagtca aaccgtcatg gttgtgttga acggagaacg ccacaatgag
      481 cgatgctcag acgcctcaaa cgttcgaccc gtattacact tggttcggaa tcccgccgac
      541 tgaacgaccg atttcctatt accgtttact tggcctctcg ccattggaat caaatgaggc
      601 agccattgaa gcggcagcgg atcagcgcct ggcttatctt cgcaccaaga cgcttggcgc
      661 tcatgctatg ttcgccgaaa gacttataag cgaagtgatc caagcacggc ttgtcttgct
      721 cgacccagtg aaaaaacagg cctacgacgc cgccctctca cgtagcctgc aggaaaggcc
      781 tgttatatcc caggaacaga caccagatag ccaacttaga cagccgcagg tggctgtcac
      841 gctcggtatg gaatcataca caccgggaga cacgcaccgc ttccgcaaga aaagtcgatt
      901 ctcaaaggaa cagtggatta aggccttagc tctcagcata tcgttctgct ttgtcctagt
      961 ggctgtggga ttatggtttt cctacaaaaa gattggccgc cagcaagatc cgaattcttc
     1021 tccgaaacag gaagagctcg ctggtgggcc agctgcacca cgggcaaacg agggttcatc
     1081 taaggaagtc ccggaacaga tccaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnntccg atggagccga gcaagacgcc cgaggagtta gcggccgaag cggcccttaa
     1261 aaaactccag gagctggagg cccactacgc ggaggccacc cggtcggccg aggcacgagc
     1321 ggcggcttgg gactttcgtg gtgcggcggc agagctgagc cagctacact ttgaagaggc
     1381 gtccttagcc agccgagtcc agcagagact ggaggaacta gagcggatgc agggcttgaa
     1441 ggaaaagctc atcacggcca tcaaccgtgc caagcctcct cttcagaaga gtcagttagg
     1501 gctgaagggt cttgggggcg aggtgattgg ggcggacgag gagggcctga cgacgcggct
     1561 gctaaacggc aagacggagc gactggaatg gtcgagtctt ggggcaagtg gtgtggagcg
     1621 gctgttttcg ccgaagcagt ttgaggcgtt ggtgggacga gatggggagg cgtggttggc
     1681 ggcgggcttg gtggcgttga ccttggggaa tgcggccgag gcggagcggt gcatgtttcg
     1741 ggccaaggag cttggggcgg cgattgagcc gtacttgagt tctttagcgg gggcggcgtt
     1801 tgggcggatc acggaggggc ttaaggcagc ccaaggggcc ttggctgtag cccggcagcc
     1861 gggtgagccc gggtcggcca aggagcgatt tgccaaggtc gagaggcagt tccggcaggt
     1921 ggcctcgcag ttggaggagc tggaaacccg ctaccggtca gccccgtggt acagtgccca
     1981 tcagcaggcg gtggccggtg ccagacaggc ggtccagcgg ggcctagacg aggcggctgc
     2041 cgagcaattg tatgccgagg cagcggagct ttttgcccag agggagtact ttgacctcaa
     2101 gccgatcgtt gagcagctca agagccagta cgcggccacc gcgccggtga gggataccgg
     2161 tcggaagccc tcgtttaccg aattggagca ggcggtggcc gatctgggcc ggtggatcgt
     2221 cgtccgtcag gacggcaagg gagacttcac ctcaatccag gcggctatcg acgctgcccc
     2281 agtgaacagt gtgatcgaaa tccaggacaa cgtgccgtac agtgaaaaac tgattattgc
     2341 tagacgacta gagattcgag gtagccgcaa atcgtggccc attctactta gccagggccg
     2401 ctcaactcca gagggttcga tggtagatat ccgcgcggag ggagtgatgg tccgccggat
     2461 cgcaatcgtc acctatgatc cttcagaaca cacgcccgct tgcgtctact ttcgaactcc
     2521 cgccagcatt aaatcgctca ttctttcggg agccaacgtt ccagcgcttc gaggtgaggg
     2581 atctgttacc ggtcagatta tcgacagtct cattctatct tcatttcgtg cactctggtg
     2641 gaaggcttca ttgcgaaact gtctcgtttt aaatggtatg gacctgttac gtaggtgcga
     2701 agttgaagga tgtgtagtcc agggtgttat ccaccttggg ggccgggagg gtcatttgat
     2761 acgcgatacg attgttttag aagtaacgat cgatgctggt atcccatacc agattgagaa
     2821 ctgtgccttt gttaaaccag ggattccggg tggagcgaag aacagtttcg tcgcaaatcc
     2881 aaggtttcgc gatccggcca ttctggacta tcgcttgatc cccgatagcc catgcatcgg
     2941 caaagcatcg gatggtgggg acatcggctg ccgctacacg ccggagatga ttgaaatgtg
     3001 caaagtggcc cttgagctcc gtgcccgtgg gatcatcaag ttctagccgc cgttacctaa
     3061 ttccttgaca agtctctgtt tttccctccg gttgcgattc caaaaagggg taaactctgg
     3121 gcgaccagtg ggtagctaac agaaaagaaa tatggcgggc cccctcgtcc cgacctcggt
     3181 cataagccac aggagagccc gtagctactc aacagctctc cccaaaaacc tacaacgtcc
     3241 ccgcagtgac gtgtttgatt cggactggac aagaagttac ttcacggaag cttagcggcg
     3301 ccacgtcaca caaatatcgt tctgcaaaat acctggggca aacggcatgg ctgcatgttg
     3361 ggccttctgc ttgatgtgat aacttgggca acggcccgaa agaattacgc agtcaccatc
     3421 gatggtgacc cgcagatcag ccaccgcacc ggccgtggcg gcttccaccg ctcgcaggat
     3481 ctgcgttgcc agccgttcgg cttctgcctg cgcgggagac aatggatatt catccagatg
     3541 cccctcgcga acaacgacgt cctcgtcaaa cggcgaatag aaggacatgg ttccctccac
     3601 gggcgcggaa atgcgatcct ttcacatccc acaccacaaa acaccgaggg ccatccctgt
     3661 tccctcattc cccagaaacg tttcaccggt cggtaggaaa ccccacacct tcaatcacga
     3721 tcgaagctat acggccgcac ttcgatctga ttgagaacct ccacattgtg atttcgcaga
     3781 accgaccgga ccgcctcctg cgccaactgt ttgtaataat aactggagac aactccagtc
     3841 agaatgatcg tatcgccctg ttgctcgacg ttgacgtcgc gcagttcatg aatggggcta
     3901 gctctcaacg ccgactctgc gcgcgcctga aggttgccgg aaagcacatc aaccaccata
     3961 gctgcttcca tccctggtgc catgaccaat gaaccaaacc tcacaccggc ccgcgccggt
     4021 cagctttcgc tgcaaaccca gagaagccat tatcaaccaa gatgctggca gcgggcttat
     4081 catctaaaat cggaacccac cgctcgtcac aggggctaaa atgcggatca aattgcggtc
     4141 gcacccaggc gtttccatct cgatctactg ggcgacgacc aagatcattt atatcaggcg
     4201 acacgcaaga gtcaacccca gagtcggcga ttcttagcct tagttgtggc cccgccacag
     4261 ctttcacgcc ccacccgcac cccagcatga tgctcgcccg ttcccaggtt cccaagggac
     4321 acttcgccca aacgaattgg gcaatcaagc ggggatggat aactccttct tgttacttca
     4381 ccccgtccaa aaggcttatg tcccttgatt ggcgaatagc ccgatcaccc ttggaagcct
     4441 tacctgtcgc caggcgtggc gccgatgatg cccttttctc ggagataatt gataacaacg
     4501 tctgccagtt cttctgggct gtggtttgcc gcatcgaggt ggatttccgg ccggagcggc
     4561 ggttcatagg gatcgtcaat cccggtgaag cccttaagct cgcctgcccg agcctttttg
     4621 taaaggccct tcgggtcgcg agcttcgcac acctctaggg gggcatccac aaagatttcg
     4681 atgaaatcgc ccggtttcat cgtggcccga acccgatctc gatcaatccg gtaggggcta
     4741 ataaaggctg tgatggcaat gatgccggcc tcgcagaaaa gcttggcgac ggccccaatc
     4801 cgccggatat tttcctcccg atcttgggca gaaaagccta agccgaaacg ttttgcgaac
     4861 tcttccccat gacgttcctt caacatggca ggcgaggcgt tcaagccatg gcggatgttg
     4921 tccccatcga ggacaaaact atgaactccc atcgcgtgca gccgatgatc caccagattg
     4981 gcgagcgtac tcttgccgga gccgcttagc cccgtgaacc acaagacaca ccctttatgc
     5041 ccattcagcc tctcccgctc ttcccgggag accgcgtgtt catgccagaa aacctttggg
     5101 tcgcagccac tcatctttta ctctcttatg catattgact aaattgtttc atcgcgatgg
     5161 tttaccccaa cggtgataaa ccccgaccat tggtcttcga tgagcgacaa ccagacgctt
     5221 ggcaggtcct cagtgatctt atgaccggtt gagccacccg ctttcggtca gacattcacc
     5281 tgcgagaggg cttccaccac aaggtcgccc acctgggagg ttgtgtagcc catttttccg
     5341 gcggcctggc ttttcatctt ggtcccggtg accacctgga tggcctggtg cacacgttcc
     5401 gccgcccgct catggcccgt gtgttccagg agcatacccg ccgcggcaat cgccgcgatg
     5461 gggttgatca tccctttccc cgtgtatttg ggagcacttc cgcccattgg ctcaaacatg
     5521 ctaacccccc cgggttcggg attgatattc gcccccgcag ccacgcccat gccgccttgg
     5581 atgatcgccg ccaaatcggt gatgatgtcc ccgaacatgt tggttgtcac aatcacgtcg
     5641 tagaactcgg ggtttttcac catccacatg cagcaggcat cgacgtggtt ataatccgtc
     5701 tgaatgtcgg gatactcctt ggccacttcc tggaatgttc gccaccagag gtcgtgacca
     5761 taggtcaaga cgttcgtctt agccacgaga gtcagccgct ttttcttgtt ccgtttacgg
     5821 gtgtattcaa aggcccagcg gatacaccgt tcgcagcctt tgcgggtgta aatggccgtc
     5881 tgaatggcaa cctcgtccgg ggtacccttt tttaagaagc ctccgatgcc cccatagagg
     5941 tcttccgtgt tttcgcggac aaccacgaag tcaatatcct caggaccttt acctgccaaa
     6001 ggtgtttcca caccgggata gagcttcacc ggtcgaaggt tgatgtattg atcgaagcgg
     6061 aatcgcagtt ccagcaggag cccacgttcc agaatacccg gcggcacatc aggatgccca
     6121 attgccccta gtaagatggc gggaaaggtc ttcagttgtt cgaagacgtt ttccggcagg
     6181 atctctttcg tccgcagata gcgctcgcca cccaagtcga actcttggag ctggagcgtg
     6241 attccttcca tctgggctac ggccttgagg accttgacgg cctcggcgat cacctccgga
     6301 ccagtgccat cgccaccaat gacggccaca cgcaggggat tgataccgct catattctct
     6361 tcacccttct ttgcttgaca ggaaaagcgt tattttacgc cgaatagtag agcgcgaaaa
     6421 gtcggccacc aaatgtgtat gccgggccaa ccaccgggga ttgcccccca ccaaccgatg
     6481 acctcctgac ggagggtccc gcgccatcgt ggaagcttcc ccgttggcga ttgctgataa
     6541 ccgggtaaac tgaaaaggtt acacttgaca cccaacgggt gggatcgtta agcagaaccg
     6601 ccatttggtc aacggtgtct tgtgttggag taacagatgc tttccggcat gtttgtgaca
     6661 tttacccaca ccagtgacgc ggtttgattt ctggcgtcac ccatggcgag atcaaatcca
     6721 cgggagatta agtccacgga gaaaatcaaa catatgaacc aggatcatcc atcgtcgtct
     6781 tcagaacagg gtcagcggcc caagagtcat cttgaggcgg cccgccggga gaagctccgc
     6841 cgtattgccg agatggggat cgacccatgg ggagggcggt tcgatggtca tcggccaatc
     6901 agggaaattc gcgcgcggga aaatgagatc gtcgtggttg acggcggcga tggtcgatct
     6961 gcgccggaac tgcgggggcc ccgggtccgc gctgccgggc ggattatgct cctgcgtcgt
     7021 gctggcaaga tcatgttcat cgatatccgc gattggactg gaaagattca ggtgctcgtc
     7081 ggcaaggcgc aggtcgggga gcggaactgg gaactggccc aatgctttga cctgggagac
     7141 attatcggtg tcgatggcga actgaaacgg acaaagaccg gtgagctaac cattttcgcc
     7201 gacaatctcc actttttgac aaagtcgatc gaaacgccgc cggagaaatg gcatgggttg
     7261 gccgacccgg aactccgtaa ccgcatgcga tacctggact tggcttacac cgaaggggtc
     7321 atggagcgct ttttgcgacg gacgcagatt gttcattcca tccgcgcgac gttggccgag
     7381 gaaggctttg tcgaagtgga aggccccacc ctgcacgtga tcgccggggg ggcagcggcg
     7441 cgtccctttg ttacccacca taacgccttg gatatggacc tttacctccg catcgccttg
     7501 gagctccatc tcaaacggct cctcgtcggg ggcatggaga aggtctatga gttgggtcga
     7561 gtctatcgca atgaggggat cagcccgcgt cacaaccccg agttcaccat gcttgaggcc
     7621 taccaggcgt acggcgatta ccacacgatg atggatttga ccgagaagct gatcgtccag
     7681 gcgatcaggg ccacgggaca gccgttgcaa cttccgtggg gagagaatgt catcgatttt
     7741 acaccaccct ttgcacggaa aacatacgat gagttgtttg ctgagcacac gggggttaat
     7801 cctaccgatc ctgcctcaat ctcctatttc gcctcctccc gcggaattga agtggtaggt
     7861 cgccacccgg acgtgatcaa gaatgagatc ttcgaagaat ttgttcagcc gaaattgaca
     7921 ggaccgattt ttgttctcga ttatccggct tcgatttgcc ctctaaccaa gaggagcaga
     7981 aacaatcctc aaatcgccga gcggtttgag ctctttgtcc aagggatgga gttggccaac
     8041 gcctacacag agctcaacga ccccgatttg caggaagaac tattcagccg ccagttggaa
     8101 ggcctcccgg aagaggagtc gatggcccgg atggaccgcg atttcattcg cgcgctgcgg
     8161 cacggtatgc caccggcggg aggattggga attggtattg accgattggt tatgttactg
     8221 acaaactgtc agagcatccg tgaggtgatt ttgtttccac tgtatcggcc ggaggtcgat
     8281 tgacgccctt tcgggtcgtc atggaggacg cccgccatgt atcgatggct tctgtgttgg
     8341 cggtatctga agacgcgcta catcgcactg gcttcaatca tcagcgtcac acttggcgtg
     8401 gccacgatga ttgtggtgaa cagtgtgatg ctgggtttta cccacgaaat gcagaaccgc
     8461 ctacatggaa tcctctcgga cctcatcttt caatcgcgga gcctcgaagg ctttcctgac
     8521 cccgactggc acatggagca aatccggcgc gtggccggag atgccatcga ggccatgaca
     8581 ccgacagtgg tagtgccggg gatgcttaat tatcaggtgg gcgacgcctg gatgacgcga
     8641 ccggtccaga tcattggggt ggatgaaaga acccaagccc aggtcagcga tttcagtaag
     8701 taccttcagc acccggagaa ccgcaaaaat ttgagcttca accttcggga aggtgggtac
     8761 gacgtgatcg accaccaggc ggatgggccg agtttgcccc gagatgaaat ggccttggcg
     8821 ggttgggaac accgccgccg cgccgcccgc ctgcgaaaga tgatcgaaca agttcaacag
     8881 gaaccggaac agcaatcccc atcgcagggc gcggaggcgt cgcccttcac taaccctttt
     8941 gagcgggccc agaccaatga gcctgtcgcc gccttcgatc ccgaaacctc tcagtacacc
     9001 ggcgccattt tggggatagg attggcaagc ttccgccgaa acggtcagga tcattttttg
     9061 cttctgccgg gagacgacgt caaactgacc cttcccacag tcggcacacc tccccgaatt
     9121 gtcagcgaca cctttacggt ggtggacttc tatgagagca aaatgagcga atacgacgcc
     9181 aactttgttt ttgtgcccat tcgggtgctg caacaattgc ggggaatgat cgacccctcg
     9241 acaggcaaag gttacgtcaa tgcgattagt atcaaattga agcccggcgc ggatatgaac
     9301 gaggtgaggg accgtttacg aaatgcgttt gccccgcagt attacgccat cgacacatgg
     9361 cgcgatagtc aagggcctct cttggccgcc gtcaatatgg aaacggccat ccttaatgtg
     9421 ttgctcttcc tcatcatttg tgtggcagga ttcgggattt tggcgatctt tttcatgatc
     9481 gttgtggaaa agacccgaga tatcggcatc ctcaaagccc tgggggcatc cgcagcgggt
     9541 gtgatgagca ttttcctggg ctacggttta cttctcggtg ttgtgggagc cggtgccgga
     9601 ctgaccttgg gccttctttt cgtccggcat atcaatgaga ttgccgacct gttggggcgg
     9661 atcacaggcc atcgggtctt tgatcccgac atttactact tttacaagat tcctacgatc
     9721 gttgatccat ggaccgtggg ctggatcatg gctggggcaa tgctgattgc ggtcttggca
     9781 agcgtgttac cggcactgcg agcagctcgg ctgcaccctg tggaggccct ccgctatgaa
     9841 taaactcacg agcacaaccg atgggctttc ctctgcctct caagagaccg gccatcttcc
     9901 gcggtctccc aatggagaac cctcagacgc ccatcctttg gtcgcacgac cgcatttttc
     9961 cctcaggaag cacgatggga gttccgagac gcttcgccaa tctcaagacc ttgagtgtgt
    10021 ggacgaacca gccgaggcga ccgagtgcct gctttctgta cgagggctcg tcaaaagcta
    10081 ccggaaggca aacgtggaaa tcccggtgct taaaggtgtt gatctcgatg tacgaccggg
    10141 cgagttcctg gccatcgttg gccagagtgg ctcggggaag agcacgcttc ttcacctgat
    10201 cggcctgctc gaccatccgg accaaggcgc cgttttcctc ggcggagacc gcatcgacaa
    10261 tgtgccggta cggatccgcg atcaattgcg caatcggacc ttcggcatgg tcttccagtt
    10321 ttatcacctc ctcccggaat tgacgacatt ggagaacatc cttgttccgg tcatgattgg
    10381 ccgatctttt tggagctact ggcgccagcg gaaggaatat cggcgtcggg ccatcgaact
    10441 ccttgagctg ataggcttgg ggcatcggct gaaacaccgc ccccacgaac tctccggcgg
    10501 agaattgcag cgggcggcca tcgcccgagc actcatcaac cgtccccaga ttctccttgc
    10561 ggacgaacct acggggaatc tcgaccccga aacaggggcg gaaatcctca accttttgcg
    10621 aaccttaagg gatgaagagc atcttacgat cgtgatggtt acccacgatc attcgattgc
    10681 cgccttggcc gaccggattg tgtgcctttc cgg
//
LOCUS       DAOSUT010000141        10597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3494_length_10597_cov_9.175204, whole genome shotgun sequence.
ACCESSION   DAOSUT010000141 DAOSUT010000000
VERSION     DAOSUT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_10597_cov_9.175204"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2832
                     /locus_tag="PLD05_12120"
     CDS             325..2832
                     /locus_tag="PLD05_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78232.1"
                     /translation="MKKIVTAFLLAAILAVPVQCACAAQGSDAESPSLIERIDRLGEK
                     VFSGVLPGKRRTTNITQENSVPSERAGSAFTSGGQASEGTTIRPRPDPLLQGGAASGQ
                     TVTTTSQSKREPTSPSPTSQVLPTRAPSVAGPAPSQNVPAARGAQASPSAEGASVADS
                     GSALYKKLMEYRTSPFASQAASRRDASGVAEPAAEVQVRSESPSSAAPVGATATLAPQ
                     PRDFANSPRVPTAASPQVRGEPTPASAVATAPKASASPPAISPSAGTAPVRSPTPAVW
                     QPSTSAVPQVPRVGVAQPSVPAATSQVPTPAAPRLGSTAAVGRSETDSVPSEEPNKPG
                     VTPPRQTVGSFSGNGTARERTADLTIPRSVQAVGSPVLLVETSGPREITLGKPGLYEV
                     FVRNIGRGVGNQVRVTVALPSWAEVVEQVPTAGTVESTTNAQKQDLVWMIQQIDPQKE
                     ERLRLKLIPRESKPIELAVQYTVLAPVNETKIEVREPKLAIRVDGPEQARFGRAEMYR
                     LEVVNTGTGEADNVVLSLLPVGTGQKTPARQTLGSIPAGEKRVVEVELIPRQTGAIQI
                     QIEVTADGGIRTQLEHVVTVIRPALEVLVTGPAMQFVNQDDRYQVVVRNPGTAPAENV
                     RVQVTCPESVEPDLTALKGAKPVGDVLEWSIPSLPAGGEVVIDIPCRFVGMGDARLMV
                     QATAEENLTTQGECLTQIQAVADLALRVEDPPRPVPVGKDGLYQLIIENRGSKAATDV
                     RVVGFFSEGIEPIAAKGIPHRVLPGQVVFEPISKIEPGERIVLEIQARAHKPGNHVFR
                     AELRCPSSDIRLAAEETTRFYDATIAERPADSSSKLR"
     gene            complement(2858..3133)
                     /locus_tag="PLD05_12125"
     CDS             complement(2858..3133)
                     /locus_tag="PLD05_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78233.1"
                     /translation="MSEELASSDAPELPVQAPSAQQVAEELSRLRRSIRRLTVLVSLL
                     TLAVFLCSAAVFGELVNYFSFDPMLYGGASAGAAILGFIFGLIVGRR"
     gene            complement(3206..4012)
                     /locus_tag="PLD05_12130"
     CDS             complement(3206..4012)
                     /locus_tag="PLD05_12130"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP78234.1"
                     /translation="MSHIRWLLVRDAVRRVSRHAAYAWLVGIGVVACSLMTAAVLAEQ
                     GGPSAAGDQEQVVDLSAKDLTGWREPHGQWAIVGEAVMDPQEPKRLAVKPGTGVLYNG
                     PQGRTVNLLSVAEHGDVIAHIEFMVPKGSNSGVYFQGRYEIQVFDSWGVEKPSYSDCG
                     GIYQRWGPQGGYEGHPPRVNASRAPGEWQTFDVIFRAPRFDENGKKIENARFVKVVHN
                     GVVVHENVEVTGPTRSAHFDDEKPTGPLMLQGDHGPVAYRNIRLRLVKLP"
     gene            4309..>4523
                     /locus_tag="PLD05_12135"
     CDS             4309..>4523
                     /locus_tag="PLD05_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78235.1"
                     /translation="MSEGSAISSQERWYRLLTGYQWFVLIVCTAGWAFDTFDQQLFNL
                     ARQPAVRDLLGSATTDVVNFYGGLATAV"
     assembly_gap    4524..4623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4624..5409
                     /locus_tag="PLD05_12140"
     CDS             <4624..5409
                     /locus_tag="PLD05_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78236.1"
                     /translation="ILPRDMIGQDPNDKDAQYLLAAALTLASRKERVTLEGALKLLDQ
                     PWTDDGSPLAKLDRKRQAQTPEDRARRKEFVEKAPVEQTSLEDHLTRLKERAKNVGGY
                     VQRWGSIQLLLFNIGAFFGIYAFSMVTDKLGRKKTFALFFLFAMVVTAFVFLTMREWH
                     HVLWMAPLMGFAQLSVFGGYAIYFPELFPTRLRSTGTSMCYNIGRYVSAFGPFLQGYL
                     SGVVFAGFAEPMRVAGATMCLFFLLGIVVLPFAPETKDQPLPE"
     gene            5538..5960
                     /locus_tag="PLD05_12145"
     CDS             5538..5960
                     /locus_tag="PLD05_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOP78237.1"
                     /translation="MKSYTQYLTFNIPARMAFVNITKQVEEAVKASGVREGLCLVNAM
                     HITASVFINDDEPGLHEDYKRWLEKLAPYDPSPQTYHHNRTGEDNGDAHHKRQIMGRE
                     VVVAITDGKLDFGPWEQIFYGEFDGRRPKRVLIKIIGE"
     gene            complement(6200..8368)
                     /locus_tag="PLD05_12150"
     CDS             complement(6200..8368)
                     /locus_tag="PLD05_12150"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOP78238.1"
                     /translation="MATRLSMCLAVTVSLTLSAVSFADENDPYYVYITTAPEFQPVKA
                     PANLALARWDTWIYMPWRYQWTIGTGDEGGQFCRDFGINGGFTDHGDGPFAWLEQWNL
                     RFYNDHTAGKGFLYLRDANQRSRFQAFQRDPRAIRQDREGLKPLDEALFGKLAELVRT
                     RVGRVKKSPMRVAYALDDEISWGIFVVPLPWRVHDDDAAYQAWLDRYYGGKGPKAQFV
                     TPDFILPQLKGRLADLDFSPLLDRITYNDSVWANFIGRLVEVANEVDPETPCGFVGGQ
                     SPNIWGGYDYAKLMKKIQFIEAYDLGSSQAIIRSFNPGNVFPQVTTHFHSDARGTAND
                     VWQSWYYFAHGNRGMIGWVEGWFDGTKPRPWLYEYRATLKELGETQGPKLFGARWVHD
                     GIAIYYSHPSIQVSWCLDMEAHGKTWVNRGNDHRLGTSHLVRKAWEYLLVDSGLQYSF
                     ISYDEVVRNGIPPEYRVLILPACYALSDIEAERIKEFCERGGTVIADFCCGLFDQHGK
                     GRKRGALDDLFGVSHDGSETARDFFGDRLWVETDQDAAYGYKRYRELFATISCQLEKG
                     YAVAERRLPVGTVRKVGRGSAVYLNLSPQRYLQYREEGTASDELRQPFLQPIHNAGIR
                     PWIWVTSEGRRPANTEVCYWKKGDRVYALVVQSAPVTGSMTGGGGAEQLKVQTISIEV
                     EFARNVRDVRNERTGESLGDGNRFRFDFPLAEAVFLSFVE"
     gene            complement(8461..>10597)
                     /locus_tag="PLD05_12155"
     CDS             complement(8461..>10597)
                     /locus_tag="PLD05_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP78239.1"
                     /translation="LPTETAQTPAPPPVTPVLAPLQPITIRPGESATCTITVDRRGLT
                     GPIAVQVQNLPPGVSASPLEIPDGQSSGELTLTADKSLGENEIAMAATVQIEVAGQKA
                     TQSLMLTIPRLALPELTVPASVFVFPGAKVPIEVGLQREGTAVPVELRLEKIPEGVSC
                     PAIQVAADAAKATLEVSATGQAAEGDHQVEVVVAVGERRASKSLTLVVRKSLFTVQCF
                     RVVTVKPGEQRDVDIPIARRGYNGPIEFAFENLPEGVTITANPVGQGANSARFHFAVN
                     PDAHERVRSVYVRATGGGFTFREAMVVRVSRQEGTGFLPKEIGFDPQLAPLFRRGSFG
                     GRLSAKSKAALCDAYGGTPESEKAVFAGLAWLASHQEADGRWSLKEYGRIIPTCDCHL
                     DAEKEKEIVDSDTAGTGLALLPFLGAGVTPESAPEEPAELARYRNGVRRGLSYLMRIQ
                     VNDPKSDKDGHLGGNTYAHAIATMALCEAYGLTGDERIQTAAQRAIKYLMSSQHKEGG
                     WRYGFREAGDMSVVGWVFLAIRSGQLAGLKIDESPLVRAGRFLDSCGVGPAPYNLSEY
                     CYQPGGEPKISLTACGLLTRQYLGWKKDNPHLLAGVKKIMTQLPPAGAQNVGSLYYYY
                     YATQVLHHMEGEEWDLWNHRMREHLIRTQERSGHRAGSWSPEGCDWGNRGGRIYATSL
                     ALMILEEYYRHLPLYRPVPRTQLTGTTNR"
BASE COUNT         2232 a         3084 c         2999 g         2182 t
ORIGIN      
        1 cctccacccg aaggtggatt cactcatggg gcaagaattc taatgtgcca aaattcaccc
       61 tattttgcaa tggggagact gccgcggagt gccaaccaat atcgctacct gccgcagaca
      121 gtggcgattc ccgcgtgccg gaaacaagag actgcctcgg cagtgtccca acatacgatg
      181 atataccgct aataggtggg gtgccgttaa agtctctcaa atcggttaat ccgtttaagt
      241 cggtacatcc gataatctct acggcgattt ctgtagaagt cggtcgcttc ctctcaaatc
      301 tgtaaaatga gcaggatagt gaaaatgaag aaaatcgtga cggctttcct tctcgcagcg
      361 attctcgccg tgccggttca gtgcgcctgc gcggctcaag gatcggatgc cgaatctccc
      421 agcctgattg aaaggatcga tcggctgggc gaaaaggtct tctccggcgt cttgcccggc
      481 aagcgacgaa cgacaaacat cacccaggaa aactctgtcc cctccgagcg ggcgggttcg
      541 gcctttacct cggggggcca ggcttccgaa ggtactacga tcagaccgcg ccccgatccc
      601 ctccttcagg gaggtgccgc ttccggccaa accgtgacta caacctcgca atccaagcga
      661 gaaccgacct ctcccagccc cacttcgcaa gtattgccca cgcgcgcacc atccgtggcg
      721 ggaccggccc cgtcccaaaa tgtcccagcc gcaaggggcg cacaggcttc accgagcgcc
      781 gagggggcat ctgtagctga ttccggttcg gccctatata agaagctaat ggaatatcgc
      841 acgtcgccgt ttgcatctca ggcggcatct cggagggacg cctcaggcgt tgcagaacca
      901 gcggccgagg tccaggtgcg aagcgagtct ccttcttctg cggcccctgt tggtgctact
      961 gcaacgttgg ctccccaacc tcgggatttt gcgaattcac cgcgagtgcc aacggcggca
     1021 agtccccagg ttcgtggaga accgaccccc gcgagtgccg tcgccaccgc acccaaagcg
     1081 tcggcatcac ctcctgcgat atccccttct gccgggactg caccggttcg gtcgccaacg
     1141 ccggctgttt ggcagcccag cacttctgcg gtacctcagg ttcccagggt cggagttgcc
     1201 caaccaagtg tccccgcggc cacatctcaa gtccccacgc ctgccgctcc gaggctcggg
     1261 agcacggcgg cggtcggtcg ttcagagacg gattccgttc caagtgagga accgaacaaa
     1321 cccggagtaa ctccgccgag gcaaactgta ggcagctttt cgggcaatgg gactgcacgc
     1381 gagcggacgg ccgatctcac tatccccaga tcggtacaag ccgtcggcag ccctgtgttg
     1441 ttggttgaga cgagtggtcc tcgcgagatc acgctgggta agccgggttt gtatgaggtt
     1501 ttcgtccgga acatcggtcg cggcgtggga aatcaggtcc gcgtgaccgt tgccctacct
     1561 tcttgggccg aggtcgtgga acaggtcccg acggccggaa ctgtcgagag cacgaccaac
     1621 gcccagaaac aggacctcgt gtggatgatc cagcagattg atccccaaaa agaggaacgt
     1681 ctgcgattga aactcattcc gcgggagagc aagcccattg aactggcggt gcagtacacg
     1741 gttttggccc ctgtgaacga gacgaagatc gaggtcaggg agccaaaact agcaatccgt
     1801 gtggatggcc ccgagcaggc ccggttcggt cgtgcggaaa tgtatcgttt ggaggtcgtc
     1861 aacacgggga ccggagaagc agacaacgtg gtcctctccc tgctgccggt gggaacaggc
     1921 caaaagacgc cggccaggca gacgctcggt tcaatcccgg cgggtgagaa aagggtggtc
     1981 gaggtcgagc ttatcccccg tcagacagga gcgatccaaa ttcagatcga agtgacagca
     2041 gatggtggga ttcgcactca acttgaacac gtggtgacgg tcatccgacc ggcgctagag
     2101 gtgttggtaa ccggtcccgc catgcagttc gtgaatcaag atgaccggta tcaagttgtg
     2161 gtgcggaatc ctgggacggc accggcggaa aacgtccgcg ttcaggtgac ctgtcccgaa
     2221 tccgtcgaac ctgatttgac ggccctaaaa ggcgccaagc cagtcggcga cgtgttagag
     2281 tggtcgatcc cgtctttgcc ggcgggcggc gaggtcgtga tcgacattcc atgtcggttc
     2341 gtcgggatgg gtgatgcccg attgatggtc caggccacgg cggaagagaa tttgaccacc
     2401 cagggagaat gcctgacaca aatccaggcg gtggcagacc tagcacttcg cgtggaagac
     2461 ccaccgcggc cagtcccagt ggggaaagat gggttgtatc aactcatcat cgagaaccgc
     2521 ggcagcaagg cggccacgga tgtccgagtc gttggattct tctccgaggg aatcgagcca
     2581 atcgcggcca aggggatccc gcacagggtc ttgcccggtc aggtcgtgtt tgagcccatc
     2641 agcaagattg agccgggtga gcgaattgtg ctggaaattc aggcgcgggc gcacaagccg
     2701 ggaaaccatg tcttccgcgc ggaactgcga tgcccaagca gcgatatccg tcttgccgcg
     2761 gaggaaacca cgcgctttta cgatgccacg atcgccgagc ggcccgcgga ttcatcctcg
     2821 aagctccgct aaagccattg gcggaacggg cacagcttca gcggcgacct acaataagcc
     2881 caaagataaa tcccaagatc gcagcgccgg ccgatgcgcc tccgtaaagc atgggatcga
     2941 aagaaaaata gttgaccagc tcaccaaaca cggcggcgga gcaaagaaac accgccagcg
     3001 tgagcagcga gaccaagacg gttaacctgc gaatgctccg ccgcagtcgg ctgagttcct
     3061 cggcaacctg ttgtgccgaa ggggcctgca caggcagttc cggggcatca ctcgaagcca
     3121 gctcttcgga catcgaagcg acctctcctc ggttgttgtt ttgtatgact tgcgcgccac
     3181 accttgtgga ccggcgtctg tcttcctagg gaagttttac caggcgtagc cgaatgtttc
     3241 ggtaggcaac gggcccatga tctccctgga gcatgagcgg tccggtcggc ttttcatcgt
     3301 caaaatgtgc cgaccgggtg ggaccagtga cctccacgtt ctcgtgaaca accacgccat
     3361 tgtggacgac tttcacaaac cgcgcgtttt cgatcttttt cccattctcg tcaaagcgtg
     3421 gggcgcggaa gatcacatcg aacgtttgcc actcgcccgg tgcccgcgat gcatttaccc
     3481 gtgggggatg gccctcatat ccgccttgcg gtccccagcg ctggtaaatc ccgccgcaat
     3541 cgctgtaact gggcttttcc acaccccagc tatcgaagac ctggatttcg tagcgacctt
     3601 gaaaatagac acccgagttg gaacctttcg ggaccataaa ctcgatatgg gcgatgacgt
     3661 cgccatgttc tgcgacacta agcaaattga ccgtcctccc ttgtgggccg ttgtacaaga
     3721 cacccgttcc cggcttgaca gcgagccgtt tcggttcttg tgggtccatc actgcttcac
     3781 caacgatggc ccattgcccg tgcggttcac gccagccggt caagtctttt gccgatagat
     3841 caacgacctg ctcttgatca cccgccgcgc ttgggccgcc ttgttccgcc aggacagcgg
     3901 ctgtcatcaa actgcaagcg acaacgccaa tgcctactaa ccaagcgtaa gccgcgtgcc
     3961 gacttacgcg acgaacagca tcccgaacca gaagccatcg aatgtgcgac atggcatgtt
     4021 cctttacatg tgaccacagc cttcggaaag cattgcatca attagaaatt aaaaggcacg
     4081 catttctccg cgtcaacacg atgccttcgc aattaacgga tgaagagttt ttgggggacc
     4141 tttgggactg gacaggccaa ctgttttggt tcataatccc actaaaattt tcctctgggc
     4201 cgagaaactc ggcggaaggc ttttgcccaa aggagaagtg tggggtttgt gcccggcacg
     4261 gcatacggtc accgctttgc cgaacaaggc gaataaaagg agaagagcat gagtgaaggt
     4321 tcggccattt ccagccagga gcgttggtat cgtctcctga ccggttatca atggtttgtc
     4381 ctgattgtgt gcacggccgg ttgggcgttt gacacgtttg accaacaact gttcaatctt
     4441 gcccgacaac cagccgtccg cgatctgtta ggatcggcca cgacagacgt tgtcaatttt
     4501 tacggcggtt tggcgacggc cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnattcttc ctcgcgatat gattgggcaa gacccgaacg acaaggacgc gcagtacttg
     4681 ttggcagccg ctttgacctt ggcatcccgc aaagaacgcg tcacgttgga aggtgccctg
     4741 aagctgctcg accaaccctg gaccgatgat ggttcacccc tggcgaagct cgaccgcaag
     4801 cgtcaggccc aaacgcccga ggaccgtgcg cggcgaaagg aattcgtcga aaaagcgcca
     4861 gtcgaacaaa caagtttgga ggaccatttg acccgcctaa aggagcgagc caagaacgtc
     4921 ggtggttacg tgcagaggtg gggaagcatc caactgctgc tcttcaatat cggggccttc
     4981 tttggcatct acgccttcag catggtgacc gacaaattgg ggcgtaaaaa gacattcgcc
     5041 ttgttcttcc tctttgcgat ggtcgtgacg gcctttgtct tcttgacgat gcgagaatgg
     5101 catcacgttc tgtggatggc cccgctcatg ggctttgccc agctttccgt cttcggcggc
     5161 tacgccattt acttcccgga attgttcccg acacggctgc ggagcacggg aacctcgatg
     5221 tgttacaaca tcgggcggta tgtttcggcc tttggtccat tcttgcaggg atacctgagc
     5281 ggcgttgtat tcgccggttt tgccgaaccg atgcgcgtcg ccggggccac gatgtgtctc
     5341 ttcttcctgc tgggtatcgt ggttctgcct tttgcacccg aaacaaaaga ccagcccttg
     5401 ccagaataag gcgacgcttg ggtgtggttt ccgcggaagg accggatcat gcgccggacc
     5461 gtggagatcg aacaagggtg ttcgtggatt gatgcgacaa ccggagatgg ctcgctcggc
     5521 ggcaaggagg atgttccatg aagagttata cgcagtattt gacgttcaac attcccgccc
     5581 gtatggcgtt tgtcaatatc accaaacaag ttgaggaggc cgtcaaggcc agtggtgtgc
     5641 gagaaggcct ctgcctggtc aatgccatgc acattacggc cagcgtgttt atcaacgatg
     5701 atgagccggg cctccacgag gattacaaac gctggctgga aaagctcgcg ccttacgatc
     5761 cgtcgccgca aacgtatcat cacaatcgga cgggcgaaga caatggcgac gcgcaccaca
     5821 aacgtcaaat catgggacgc gaggtagtcg tcgccatcac cgacggcaag ctcgatttcg
     5881 gcccttggga gcaaattttt tacggcgaat tcgatggccg ccgccccaag cgggtcctca
     5941 tcaagatcat tggcgagtaa atggcgccgt aatagcctcg ggagggttgc cgtccgcggc
     6001 gagcaattgg ctcggggatt ggttgtgttt tggggcaggc acaggggcct gcccctacag
     6061 gtttcaatgg gttcggcaac cgtaggggca accccctgtg gttgccccgt ttgtctgtag
     6121 cgccggtgca cacgggacgg cgaggacgcc gtccctccca ttttcgggag ttttccagcc
     6181 gtcccgctca ttctcgggac tattccacaa agctcaagaa gaccgcttct gccaagggga
     6241 agtcgaaccg gaagcgattg ccgtcaccta acgattcccc ggttcgctca tttctcacat
     6301 ctcgaacatt gcgagcgaac tccacttcaa tagagatcgt ttgcactttg agctgttcgg
     6361 ccccgccgcc acccgtcatg cttccggtga caggcgcact ctgcacaaca agggcataca
     6421 cccggtcccc ctttttccag tagcaaactt cggtgttggc cggtcggcgg ccctcgctgg
     6481 tcacccaaat ccacggccga atgccggcat tgtggatagg ctgcaaaaac ggttggcgga
     6541 gctcgtcgct ggccgtgccc tcttcacgat actgaagata tcgctggggt gaaagattga
     6601 gatacaccgc cgagccgcgc ccgacctttc gtacagtccc gactggcaaa cgtctttcgg
     6661 ctacggcgta gcccttctca agttggcacg agattgtcgc gaaaagttcc cggtaccgtt
     6721 tgtagccgta ggccgcatct tgatctgttt ccacccacag ccgatcacca aagaaatccc
     6781 gggcggtttc gctgccatca tgagaaaccc caaacagatc gtccaaggct ccccgtttac
     6841 gccccttgcc gtgttggtca aaaagtccac agcagaagtc ggcgatgacc gttccacccc
     6901 gttcgcaaaa ctctttaatc cgctcggcct caatatccga caatgcgtag caggccggaa
     6961 ggatcaacac ccgatactcc ggcgggatac cattccggac aacttcatcg taagagatga
     7021 acgaatactg aaggccgctg tcaaccaaaa ggtattccca tgccttgcgg acgagatggg
     7081 atgtccccaa gcgatggtcg ttccccctat tgacccatgt tttgccatgg gcctccatgt
     7141 ccaaacacca agagacctga attgagggat gtgagtagta gatggcgatc ccgtcatgaa
     7201 cccagcgagc gccgaacaac ttcggtcctt gggtttcgcc tagctctttc agcgtggcgc
     7261 gatattcgta aagccatggc cgtggttttg ttccgtcaaa ccagccttcg acccagccga
     7321 tcatgcctcg gtttccatgg gcaaaatagt accaactttg ccaaacatcg ttggctgtgc
     7381 ctcgggcatc ggagtgaaaa tgcgtggtca cttgggggaa gacattgccc ggattaaagg
     7441 accgaataat cgcctgcgat gatcccaggt cgtacgcctc gataaattga atcttcttca
     7501 taagttttgc gtagtcgtag ccgccccaga tgttcggtga ctggcctccc acaaagccgc
     7561 acggcgtttc gggatcgact tcgtttgcca cttccaccaa ccggccgatg aagtttgccc
     7621 aaaccgagtc gttgtaggtg attcggtcga gaagtggcga aaagtcgaga tcggccaggc
     7681 ggccctttag ctggggcagg ataaagtcgg gcgtgacgaa ctgggccttt ggcccttttc
     7741 cgccgtaata ccggtccagc catgcctggt aagccgcgtc atcgtcgtgg acacgccaag
     7801 gcaagggaac cacgaagatg ccccaggaga tttcgtcgtc taacgcatag gcgacacgca
     7861 tgggactttt tttcacccta ccgacccgcg ttcttaccaa ttctgccaat tttccgaaga
     7921 gcgcctcatc aagtggttta agcccctcac ggtcctgacg aatggcgcgg ggatcccgct
     7981 gaaaggcctg aaatcttgat cgctggttgg catctcgcag gtaaaggaag cccttgccgg
     8041 ccgtgtggtc gttgtagaat cgcagattcc attgctcaag ccatgcgaaa ggtccatcgc
     8101 cgtgatctgt gaatccgccg ttgatcccga aatcgcggca aaactggccg ccttcatcac
     8161 cggtgccaat tgtccattgg tagcgccagg gcatgtaaat ccaggtatcc caacgggcga
     8221 gggcaaggtt tgccggggcc ttgaccggtt ggaattccgg cgccgtggtg atgtacacgt
     8281 aatagggatc gttctcgtca gcaaatgaaa ccgccgaaag cgtcagagag acggttaccg
     8341 caaggcacat gcttaagcgt gttgccataa taggacctcc ggactacctg gttgcgggag
     8401 tcgggcgatg cggaaatgca cgtcgctttt gcaacgacta ttgtaacgga tgggccggcg
     8461 ttagcgattt gtggtccccg tcaattgtgt ccgtgggaca ggccggtaaa gcggaaggtg
     8521 ccggtagtat tcttccagga tcatcagggc cagggatgtg gcgtaaatcc gtccgccccg
     8581 attgccccaa tcgcagccct cggggctcca gcttcccgcg cgatggccgg agcgctcttg
     8641 ggtgcggatc aggtgttccc gcatgcggtg attccaaagg tcccattcct cgccttccat
     8701 gtgatgaaga acctgcgtgg cgtagtaata gtagtaaagc gacccaacgt tttgtgcccc
     8761 ggcgggcgga agctgggtca tgatcttctt cacaccggcc aaaagatggg gattgtcctt
     8821 cttccatccc aagtactgcc gggtcaggag cccgcacgcc gtcaaggaga tcttgggttc
     8881 gccgcccggt tgatagcaat actccgatag gttgtagggt gccggtccca cgccgcagct
     8941 atcgaggaag cgtccggctc gaacgagtgg gctttcgtcg attttaagac cggccaattg
     9001 gccgctccgg atcgccagaa agacccaacc caccaccgac atatcgccgg cttcacgaaa
     9061 accgtagcgc cagcccccct ctttgtgctg agaactcatg aggtacttaa tcgcccgttg
     9121 ggccgccgtc tgaattcgtt catctccggt tagcccataa gcctcacaaa gagccatcgt
     9181 agcgattgca tgggcgtagg tgtttccgcc caaatggccg tctttatcgg acttgggatc
     9241 gtttacctgg attctcatca gatacgacag gccacgccgt acgccattac gataacgcgc
     9301 caattcagcc ggttcttccg gggcactctc cggcgtaacc cctgcgccga ggaacggcag
     9361 cagggccaag ccagttcctg ccgtatcgct atccacgatc tccttttctt tttccgcatc
     9421 gaggtggcaa tcgcaggttg ggatgatgcg gccatactcc ttcagcgacc aacgtccgtc
     9481 tgcctcctga tgggacgcca accaagcgag accggcaaaa acggccttct ccgattccgg
     9541 cgtcccacca taggcgtcgc ataaggcggc cttactcttc gcgctcagtc ggccaccgaa
     9601 tgacccacgt cggaaaagcg gtgccaattg ggggtcgaag ccgatctctt tcggcaaaaa
     9661 gccggtccct tcctggcgag agacgcgaac aaccatggct tcccggaacg tgaacccgcc
     9721 ccctgtcgca cggacataga ccgagcgaac acgttcatgg gcgtcgggat tgaccgcgaa
     9781 atgaaatcga gcggaattgg ccccttggcc caccgggttc gcagtgatgg tcacgccctc
     9841 cggtaggttc tcaaaggcaa actcgatcgg gccattatag cctcgccggg caatgggaat
     9901 gtcaacatcc cgttgctcgc cgggtttgac ggttaccacg cggaaacact gcacggtaaa
     9961 aagactcttg cggacaacga gcgtcaaaga cttggatgcc ctccgttcac ccactgcgac
    10021 aaccacctcg acctggtgat caccctcggc agcttggccc gtggctgaga cttcgagtgt
    10081 cgccttggcg gcgtcagcgg ccacctgaat ggccggacag ctcacgcctt cagggatctt
    10141 ttcgagacgc aactccacgg ggaccgccgt gccctcacgt tggaggccca cttcaatcgg
    10201 caccttggcc cccgggaaca caaagaccga ggctggtacg gtcagttcgg gaagggccaa
    10261 tcggggaatg gtcaacatga gcgattgcgt cgccttttgg ccggcaacct caatctgcac
    10321 cgtcgcggcc atggcaatct cgttttcgcc caaacttttg tcggctgtga gggtcaattc
    10381 accgctcgac tgtccatctg gtatctcaag cggactagca gaaacgcccg gtggcaggtt
    10441 ctgtacctgc acggcgatcg gccccgtcaa accccgccga tcaacggtga tggtacaagt
    10501 cgcggattca cctggtcgga tggtgatcgg ttggagcggt gccaaaacag gtgtcaccgg
    10561 gggtggggcc ggtgtttgcg cagtctcagt aggcagt
//
LOCUS       DAOSUT010000142        10393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3580_length_10393_cov_9.331979, whole genome shotgun sequence.
ACCESSION   DAOSUT010000142 DAOSUT010000000
VERSION     DAOSUT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_10393_cov_9.331979"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLD05_12160"
     CDS             <1..546
                     /locus_tag="PLD05_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78240.1"
                     /translation="CRKLLGFNNARDAARYAMEELLTDNYMGANYVCADANYAAVVYG
                     GDEVKIVEIPPGLHLLTNGDLDDPNDPRQAFARRLLTLQKLDSAVAFLAVASRTFARR
                     CDAEGRWGVVFTRGNFGTVSSTLLSLTKKTHHAILQHAPGPPDQVAYEDFSALLRQVL
                     SSDRSRSAQLAKNPAASPGVP"
     gene            1203..1793
                     /locus_tag="PLD05_12165"
     CDS             1203..1793
                     /locus_tag="PLD05_12165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOP78241.1"
                     /translation="MSVLVSREAPNFKATAVMPDGSFKEISLQDYRGKYVLLFFWPLD
                     FTFVCPTEIIAFSDRADEFEKLGVQILGCSIDSHYTHLAWRNTPRKAGGIGEVKYPLI
                     ADLDKKISEAYGVLLPNGIALRGLFFIDKDGIVRHEVVNDLPLGRSVDEALRIVKAWQ
                     FYEQHGEVCPANWHEGDPTIKPDPKASTEYFSKVYK"
     gene            2127..2819
                     /locus_tag="PLD05_12170"
     CDS             2127..2819
                     /locus_tag="PLD05_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOP78242.1"
                     /translation="MMALGTDPQLAVIFDMDGVLIDSYWAHFESWRIVAAEEGLQITE
                     EQFSRTFGRTSREIIAALWGDKTPGEERIRELDERKEAAFRDLIRDNFPIMSGAPELL
                     DALHREGFRVAIGSSGPPENVALTVEKLGRSNIFGAIITGKDVTRGKPDPQVFLLAAE
                     RLNVPPRWCAVVEDAPPGVEAAHRAGMKAVGFASTGRTPEMLAQADLIVTSLFELSPQ
                     RLRDLILGGRST"
     gene            3174..4130
                     /locus_tag="PLD05_12175"
     CDS             3174..4130
                     /locus_tag="PLD05_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP78243.1"
                     /translation="MSEKQVSVATVETGTTKSAPEVVIETRTLSKVYRDFWGRTKVQA
                     LKALDLQIFRGEVFGLLGPNGSGKTTTMKLLLGLLFPTSGEVYVFGRPATDVSKNERI
                     GYLPEESYLYRFLNAEETLDFYARLFSMPRSVRRQRVADLIEMVGLTWAKRRPLKEYS
                     KGMTRRIGLAQALINDPDLILLDEPTSGLDPIGTREMKDLILRLRDQGKTIVMCSHLL
                     ADVQDVCDRIGILYQGELKELGRVDQLLALKDITEIRVPRMSPDAEQEVKAVLRKHGL
                     EVLAWGNPTTTLEDLFLRIIRESEEHPGLRVHGRGDGHADRN"
     gene            4114..4923
                     /locus_tag="PLD05_12180"
     CDS             4114..4923
                     /locus_tag="PLD05_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78244.1"
                     /translation="MPIETDLPEFSTWILPALERWLLAGLGLLTLCCFVGLLWCLLLY
                     RRHGWRRFGDLLVGGFLDLVRVNGRRIGALAWLTVKEALGRRVTVVVLIFLLGLLFAG
                     WFLDPASTEPHRLYLNFVLGTTNYLVLLLMVFLTAFRLPSDIQNKTIYTVLTKPVRTV
                     EIVTGQVLGFIVVGSILLGIMWIVSYPFVVRGLYHTHQVNVDELRPLGEPVTLPDGTT
                     ATALQGWTASTWQHRHRFFVDANGNPRVDRERGHYHNVTVVGRLMVRPRMF"
     gene            4953..6038
                     /locus_tag="PLD05_12185"
     CDS             4953..6038
                     /locus_tag="PLD05_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78245.1"
                     /translation="MPVYAKKLTFRDRGGLDTEKGISVGDEWIYRSYIEGGTEAAGIW
                     TFENLRPEDFPGDTIPVEMNIGVFRTHKGDIERGILGSLLIRNPENGLTVELQVFESK
                     EFSILELQIPKTIRKFSSVRFVQRRAKAPDGSIQYSPAQPLNPELLSRTEFDLFKDFV
                     VDGKVEVWLQCLESGQYFGMAKPDLYIRAGNRSFFANLFKGYLGIWLQMVVLVGFGVT
                     LSTFLGTPVTLVSTLGVMIAGMAHQFMVQIVTGELYPEVETPWRGGPLESLIRLVLQE
                     GVMTDLPQTAGTVIARAVDVVFLLWMDLMAQIIPPMERLSLINFVSYGYDVSLSLLAV
                     NAITVVGYLIPLVIVGHFFLRSREVAA"
     gene            6035..8206
                     /locus_tag="PLD05_12190"
     CDS             6035..8206
                     /locus_tag="PLD05_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78246.1"
                     /translation="MTSRQLFIRKIIYLIIMAVLLGVLAYLGTPAAPSRDPQKASPGG
                     VLAQVRELEGLTPTQLGQLDPGGEAIKLATLGMRGVAVHILWMKAEDYKRRKDWSHLS
                     ATLEQMVKLEPHFITVWRHQGWNLSYNVSVEFDDYRERFRWVIRGINFLKEGVRYNER
                     EPILYGDVGWFYSQKIGRADESKQFRRMFMDPTDPFDIHGGRPPRDNWLVGKDWYAEA
                     ERLVDQGAILRTTPLVFHSHRPMCQMNYAEAIEKEGTFGDTAIAAWQTAWHEFVNEFG
                     HRELPSGFDDYPIIRLYEEDILRTQAAELAKKIEELEPGLRNKLLEERRRELTPEERE
                     AYDTPEHDRTVGQAELAFSASIKMKLTHEQVARRISDPKARRQALELARKAMALERQA
                     DIIAHNQQIVNYDYWKRHAEVEQQPEAVAARELMYRSEQAYYNNDHVTAMELFFEAAK
                     KWREVIEKNPDIQYDDHTINDLVEFVDRYMKVLDQLDAIFPEDFALSDIIRRRVRRRP
                     EYQAAQDKLASARKAISKNDFTAARADLEDAIKQYRGMMEFIKCLKLLADRATLNDIM
                     AVIEAYDDLLAKLGDVFPRNFELAELIRLQCLHTPEMRAIKRSVTQAEYLLMQGTGPQ
                     QLAAARQQLEQAVPLAATVLKKFPHLIRLSDRRTAMELAELDLRYRQVLARLGESLPQ
                     DYPLQEFNQKVVPILQKEASEQEGTTPSSTPAATSTSDKKT"
     gene            8576..9682
                     /gene="serC"
                     /locus_tag="PLD05_12195"
     CDS             8576..9682
                     /gene="serC"
                     /locus_tag="PLD05_12195"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOP78247.1"
                     /translation="MVKRVYNFSPGPAMLPLPALEEAQRDLLALPGVGMSILEISHRS
                     ATFKGIIEAAEENLRRLLNIPDDFEVLFMQGGARLQFGMIPMNFLRGKDRPASYVVTG
                     SWSKLACQEAQLVGQVHVAWDGKATNYDRVPSDDQLDIKPQSAYVYICSNETIGGVRF
                     PREPETGGIPLVCDASSELLSRPVDMSRYGILYACAQKNLGPAGVTVVIIRKDFVEAC
                     PDDLPSMLSYKKFAAEKSLLNTPPVFAVYMVKLVTDWLIREFGDLKRVADYNEEKANL
                     IYDVIDRCGGFYQVHAAPGSRSTMNIAFRLPTQELEKEFLKQAEARNLYNLQGHRSVG
                     GCRASIYNAMPREGVVALRDFMLEFFEENRHRLA"
     gene            9777..>10393
                     /locus_tag="PLD05_12200"
     CDS             9777..>10393
                     /locus_tag="PLD05_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOP78248.1"
                     /translation="MHEDLLGKLTPAQREAVTHVEGPLLILAGPGSGKTRVVTFRIAY
                     LLRLGIPDKNILGLTFTNKAADEMRSRVEALVPGARVWLGTFHRFAAQTLRQYAQFVG
                     LEPNFTIYDKENSLRALKAVINDANIDLGSYSVESVAHAISTAKSALILPTDYLPREG
                     SPLGELVRRIYPLYQQRLIQSNAVDFDDLLMHLAILLRQHPEIRKR"
BASE COUNT         2148 a         2899 c         3029 g         2317 t
ORIGIN      
        1 tgccgcaagc ttctcggttt taataacgcg cgtgatgcgg cccggtatgc catggaagaa
       61 ttgctcaccg acaactacat gggggccaat tacgtgtgcg ccgacgccaa ctatgcggcg
      121 gtggtctatg gcggcgacga ggtcaagatt gtggaaatcc ctccgggact acaccttttg
      181 accaacggcg accttgatga tcccaatgac ccccgacagg ccttcgcccg gcgactgttg
      241 acgcttcaaa aactggactc cgccgtggcc ttcttggccg tggccagccg cacgtttgcc
      301 cgccggtgcg acgccgaggg acgttggggg gttgtcttta cccgtggcaa tttcggcacg
      361 gtcagttcga cgctcctttc gttgacaaag aagacccatc acgccatcct ccagcatgct
      421 ccgggtcccc cggatcaggt ggcctatgag gacttctcgg ccttgttgcg gcaggtcctt
      481 tctagcgatc gttcgcggtc cgcacagttg gccaagaacc cggccgctag tccaggcgtg
      541 ccgtgatcgc ggcagcggcc ccaaggacgc gcaacttggc ccgccggctc ggctgggtcc
      601 cctcgatttg ttgagatcgt taatctaaga gcctatccca taatctcctc tcgcctgttc
      661 ggcgggagag gggtcggggg taagggtgct tcgaaccgcc gccggttttc tcaatcgatt
      721 ccgacgatcg cttgccctca ccccaaccct ctcccggggg gagaggggtc ttttgggata
      781 gcctttagat ggaaagtttg tcactcggtc ccatcggcca ggtgaaacgc acccgtccga
      841 gtgacgcgca gaacttccgg gtgccggggt gagtttgtat cctcggccgg ctcctcgggg
      901 agtttgccaa catggttcgc aaaatgtccg ccgtgttgtc gatccggcat acggcggttt
      961 tttgtgcgcg gcgctggctg atctcttttc gcaattgccg ctagggattg aatttacgca
     1021 atttcatcaa cggaaaatgt atcaccaatc aggcggttgc acggggaaag ataatcgagt
     1081 tcttgactta tgattgctaa ctgcgctaat tctttgatac ccgacggtgt gtgtcgtggg
     1141 acgtcgggtg gaacgttggt cgtttcttca gcaactactt acggaatagg aggtttctag
     1201 gaatgtctgt gttagtctcc cgggaagcac ccaatttcaa ggccacggcc gtcatgcccg
     1261 atggttcgtt taaggaaatt tcgctccagg actatcgtgg caagtacgtc ctgttgtttt
     1321 tctggccgtt ggacttcacc tttgtgtgcc ccacggaaat catcgcgttt tccgaccgag
     1381 ccgatgagtt cgaaaagtta ggcgtccaga tccttggctg ctccatcgac agtcattaca
     1441 cccaccttgc ctggcggaat acgccgcgaa aggccggcgg cattggcgag gtcaaatatc
     1501 ccttaattgc cgacttggac aagaagattt cggaagccta cggggttttg cttcccaacg
     1561 gcatcgccct gcgaggcctg ttctttatcg acaaagacgg cattgtccgg catgaggtcg
     1621 tcaacgacct gccgctgggg cggagtgttg acgaggccct gcggattgtc aaggcgtggc
     1681 agttctacga acaacatggt gaggtctgtc cggccaattg gcatgagggc gatcccacca
     1741 tcaagccgga cccgaaggcc agcacggagt acttctccaa agtttacaaa tgaggcgttg
     1801 atgcgatcgc ttcgcccgat ggggtgagcg agagaacgcc cctatgatcc gcaggggcaa
     1861 cccgcgggtt gccccttttt cttgtcgcca tgttccatca aatgtggacg cacatgcgtc
     1921 atctcctcgg tcctcgtaag cgtcggtgaa ccttgcttgc tcccgaccag aacggtgcgg
     1981 ccccaacgcg tcggccgggg tcgctttgtt ttcttgccta cttgcttcac aatagacggt
     2041 tcgttcactg atataggtgg caatttccgt gggcaggatt aaaccggcta ttgattgaca
     2101 ttgtttcgca ggcgagggag ttccccatga tggcactcgg cacggacccc cagttggccg
     2161 tcatttttga tatggatggc gttttgatcg actcctattg ggcccatttt gagagttggc
     2221 ggattgtcgc ggccgaagag ggattgcaaa tcaccgagga gcagttttcc cgcacttttg
     2281 gccgcactag ccgcgagatc atcgccgcgc tgtggggcga caagaccccc ggagaagagc
     2341 ggattcgcga gctggacgaa cgaaaggagg ccgcctttcg ggatttgatc cgcgataact
     2401 ttcccataat gtcgggggcg ccggaactgc tagatgccct ccacagggag ggattccgag
     2461 tggcgattgg ctcgtcaggt cccccggaaa acgttgccct gacggtagaa aaactcggtc
     2521 ggtcaaacat ctttggggca attattacgg gcaaagatgt cacccgggga aagcccgatc
     2581 cgcaggtgtt cctgctggca gcggagagac tcaacgtacc gccgcggtgg tgcgccgtgg
     2641 tagaagacgc ccccccaggg gtcgaggccg cccatcgtgc ggggatgaag gccgtgggtt
     2701 ttgccagtac cggccgaaca cccgaaatgc tcgcccaagc cgacctaatt gtcacgtcct
     2761 tgtttgaact atcgccccag cgactccgcg acctcatcct tggcggtaga tcgacatagc
     2821 ggccgtcaac accgcaccaa ttcgtctgac acccgggacc aacggtcctc cggcaggggc
     2881 tacgtacgcc aattaattgc cgactttttg ccgtcggttt atagtggaag aaaacgtcag
     2941 gggcaaaaaa cgtcaccaaa gggcccacga gcccgccagc gactgttttg cgaccttagt
     3001 actccaccta aagggggata ttggacactc tactggttta ttctagatca ttccaggagg
     3061 gaagcggcga tctgaaaggc ttgcgtacga acctttgaac agccgtcaaa ggtcatgcgt
     3121 aaagcgccac agtcggcaag ggaaagatct tatcggatcg aaggttacaa ctcatgagcg
     3181 agaagcaagt ctctgtagcc acagttgaga cgggaacgac caagtcggcc ccggaggttg
     3241 tgatcgagac gaggacgctc tccaaggtct atcgggactt ttggggacgg actaaggtcc
     3301 aggccctgaa ggcccttgac ttgcagattt ttcgcggtga ggtgtttggc ctcctggggc
     3361 ctaacgggtc gggcaagacc accacgatga aattgctgct cggcctgctt ttccccacat
     3421 cgggggaggt ttacgttttt ggacggccag ccaccgacgt ctctaaaaac gaacgcatcg
     3481 gctacctgcc tgaagagtcg tacctctacc gtttcttaaa tgctgaggag accctggact
     3541 tttatgcccg gctcttcagc atgcctcgat cggtgcggcg gcaacgggtg gcggatctca
     3601 tcgaaatggt gggcctgact tgggccaagc ggcgtccact caaggaatac tcgaaaggga
     3661 tgacacgccg catcggcctg gcccaagccc tcatcaatga tcccgatttg atcctactgg
     3721 acgagccgac ctccgggctt gacccaatcg ggacgcggga gatgaaggat cttatccttc
     3781 gcctgcgcga ccaaggcaag acgattgtca tgtgcagcca cctgttggca gacgtgcaag
     3841 acgtctgtga ccggatcggg atcctttatc agggagaact caaggaactg gggcgggtcg
     3901 atcaactctt ggccctgaag gacattacgg aaatacgcgt accgcgaatg tcacccgacg
     3961 ccgagcagga ggtcaaggct gtcttgagga aacacggctt agaggtactg gcctggggca
     4021 atcctacgac gacgttggag gatctcttct tgcgaatcat tcgcgagagc gaagagcatc
     4081 cgggacttcg ggtacatggt cgaggtgacg ggcatgccga tcgaaactga tctcccagaa
     4141 ttctccacat ggattctccc ggcccttgaa cgatggttgt tagctgggct cgggttgttg
     4201 actctgtgct gcttcgtcgg ccttttgtgg tgcctccttc tttaccgtag gcacggttgg
     4261 cggcgattcg gggatttgtt ggtggggggc tttctcgatc ttgtccgggt caacgggcga
     4321 cgcatcggtg cgctggcctg gttaactgtc aaagaggccc tggggcggcg ggtgacggtg
     4381 gtcgttttga ttttcctcct cgggctgctg tttgcgggat ggttcctcga tcccgccagc
     4441 accgagccgc accgacttta tctcaacttc gtgttgggca cgaccaatta cttggtcctt
     4501 ttgttgatgg tcttcctcac cgcattccgg cttcccagtg acatccaaaa caagacgatt
     4561 tacacggttc ttaccaagcc ggtccgaaca gttgagatcg tcaccgggca ggtgttgggg
     4621 ttcatcgtgg tgggaagtat cctgctcggc atcatgtgga ttgtcagtta cccctttgtg
     4681 gtgcggggcc tttatcatac gcatcaggtc aacgtggatg aattgcggcc tcttggggag
     4741 ccggtgacgc tgcccgacgg caccacggcc acagccttgc aaggatggac ggcttccacc
     4801 tggcaacacc gccatcgctt ttttgtggat gccaacggta atccccgggt cgatcgggag
     4861 cgtggccact accacaacgt gaccgtggtc ggtcggctga tggtaagacc tcgtatgttc
     4921 taggtggtcc tgaaggtcaa ctcgttgccc gtgtgccggt ttatgccaaa aaactgacgt
     4981 tccgtgatcg cggtggcctt gatacggaaa aaggcatcag cgtgggcgat gagtggattt
     5041 accgcagcta tatcgaaggc ggcaccgaag cggcgggcat ctggacgttt gagaatcttc
     5101 gtcctgagga cttccctggt gacaccatcc ccgtggaaat gaacatcggt gtttttcgta
     5161 cccacaaagg cgacatcgag cggggaatct tgggcagtct gttaattcgc aacccagaaa
     5221 acggactgac tgtcgaactc caggtctttg agtcgaagga gttcagcatc ctcgagttac
     5281 aaatccccaa aacgatccgc aagttttcca gcgtgcgatt tgttcagcgg cgggcgaaag
     5341 cgcccgatgg ttccatccag tatagcccag cccaaccgct caatccggaa ctcctttccc
     5401 ggacggagtt cgacttgttc aaagacttcg tcgtcgatgg caaggtggag gtatggctcc
     5461 agtgcctaga aagtggccaa tattttggca tggccaagcc cgacttgtat atccgggcag
     5521 gaaaccgctc gttttttgcc aacctcttta agggctattt gggcatctgg ttgcaaatgg
     5581 tcgtcctcgt gggctttggc gtcactttaa gcaccttctt gggaacaccg gtcacactgg
     5641 tctccacctt gggggtgatg attgccggga tggctcatca gttcatggtg cagattgtca
     5701 ccggggaact ttatccggaa gtggaaaccc cttggcgggg aggaccactt gagtcattga
     5761 ttcgattagt tcttcaagag ggggtgatga cggatttgcc ccagaccgcc gggactgtca
     5821 ttgctcgggc agtggacgtt gttttcctcc tatggatgga cctgatggcc cagatcattc
     5881 cgccgatgga gcggttaagc ctgatcaact ttgtttctta cgggtatgac gtcagtttga
     5941 gtctcctggc agtcaatgcg atcacggtcg tggggtattt gatccccctg gtgattgttg
     6001 gccacttctt cctgcgatct cgtgaggtgg cggcatgacg tcgcgtcagc tatttatccg
     6061 aaagatcatt tacttgatta tcatggcggt tttgctgggt gttctggcct atctgggcac
     6121 acccgctgca ccgtcgcgcg acccgcaaaa ggccagtccc ggtggcgtgc tggcccaagt
     6181 ccgcgagcta gagggcctca cgcccaccca attgggacag cttgatcccg gtggcgaggc
     6241 catcaagttg gccacgctgg gcatgcgcgg cgttgccgtg cacatcttat ggatgaaggc
     6301 cgaggattac aaacgccgca aggactggtc gcatctctcg gccacgttgg agcagatggt
     6361 caaacttgag ccccacttca tcactgtttg gcgacatcag ggctggaatc tgtcttacaa
     6421 cgtctcggtt gagttcgacg actatcggga acgcttccgc tgggtcatcc gcgggatcaa
     6481 tttcctcaag gagggtgtcc gttataacga acgcgagccc atcttgtatg gtgacgttgg
     6541 ctggttttat agccagaaga ttggccgggc ggatgaaagc aagcaattcc gccgcatgtt
     6601 catggacccc acagacccct tcgatatcca cggaggacgg ccgccgcggg acaactggtt
     6661 agtggggaaa gattggtatg ccgaagccga gcgcctggtc gatcaagggg ccattttgcg
     6721 gaccacgccg ctggtcttcc attcgcaccg ccccatgtgc cagatgaact acgcggaagc
     6781 cattgagaaa gaggggacct ttggcgatac cgccatcgcg gcatggcaaa cggcgtggca
     6841 tgaattcgtc aacgagttcg gccaccgaga gctgccgagc ggcttcgatg attatcccat
     6901 cattcggctg tacgaagagg acattctcag gactcaagcg gctgagctgg ccaagaagat
     6961 cgaggaattg gagcctggcc tccgcaacaa gctgttggag gagcgacgtc gtgaattgac
     7021 tcccgaggag cgcgaggcgt acgacacccc ggaacacgat cgaacggtag ggcaggcgga
     7081 gttggcgttc tcggctagca tcaaaatgaa gctgacacat gaacaggtgg cgcggcggat
     7141 ttccgacccc aaagcgcggc ggcaggccct cgagcttgcc cgcaaggcga tggccttaga
     7201 acggcaggcc gacatcattg ctcataacca acagatcgtc aattacgatt actggaaacg
     7261 ccatgccgag gttgaacaac agcccgaggc cgtggccgca cgggaactca tgtatcgttc
     7321 tgagcaggcc tattacaaca acgaccacgt gacggccatg gagctgtttt ttgaggctgc
     7381 caagaagtgg cgggaagtca tcgaaaagaa tcctgacatc caatatgacg atcacacgat
     7441 taacgatctg gtggaattcg tggatcgcta catgaaggtc ctcgaccagt tggacgccat
     7501 cttccccgaa gattttgccc tttcggacat catccggcgg cgcgtgcgtc ggcgtcctga
     7561 gtaccaggcg gcccaggata agctcgcttc cgcccggaaa gccatcagca aaaatgactt
     7621 cacggcggcc cgcgccgacc tcgaagatgc catcaagcaa taccgcggca tgatggagtt
     7681 catcaagtgc ctgaagctcc ttgccgatcg agccaccttg aatgacatca tggccgtgat
     7741 tgaagcctac gatgatcttc tggccaaatt gggagacgtg ttcccgcgga atttcgaact
     7801 ggcagagctg atccggttgc agtgtctgca cacgccagaa atgcgggcga tcaagcggtc
     7861 cgtcacgcag gcggagtacc tcctgatgca ggggacaggt ccgcagcaat tggcagcggc
     7921 acgtcagcag ctcgaacaag ccgtgccttt ggcggctacc gtgcttaaaa aattcccgca
     7981 cctcatacgc ttatccgatc gccggacggc gatggaactg gcagagcttg atctgcgata
     8041 ccgtcaagtc ctggcaagat tgggcgaatc tctgccccag gattacccac tccaggaatt
     8101 caaccaaaag gtcgtgccga tcctccagaa ggaagcctcc gagcaagaag gtacaacgcc
     8161 atcgtccacg cccgcggcca cctccaccag cgataagaag acgtgacaca ccccgtggag
     8221 gttgatctgc cctccaaggc gtgacacggc tttcactgcg tgggctggtt cattcgtaca
     8281 gccgccggtg caagctggtg gtgtggtatg ttcgctgcgc gttcaccatc tttctcttcg
     8341 aagtggcatc ggccttacgt cccccaattg tccaaacccg tgggggaggt tacgctttga
     8401 agaagcgctc agatttggcg tgccctttgt tcggcacaat tgtgacgtga agttgctctc
     8461 tgtggcacgc cgtcatgcaa ggagaacctg gtccgggcaa gatttgtcta attcgattta
     8521 cacacgatca ggctgcatcc cagacataaa tgttcaatga tcgaggagag acgtgatggt
     8581 gaaacgagtc tataactttt cccctggccc agccatgctc cctttgccgg cactggagga
     8641 agcacagcgt gacctccttg cccttcccgg tgtagggatg tccatcttgg aaatcagcca
     8701 tcgttcggcg accttcaagg ggattatcga ggcggccgag gagaatctcc gtcgtttgct
     8761 gaatattccc gatgatttcg aggtcctgtt tatgcagggc ggtgcccgat tgcaattcgg
     8821 gatgatcccc atgaattttc tccgcggcaa ggaccgtccc gcgtcctatg tggtcacggg
     8881 gtcctggagc aaactcgctt gccaggaggc ccaactggta ggtcaggtcc acgtggcctg
     8941 ggatggcaag gccaccaatt acgaccgcgt gcccagcgac gatcaattgg atatcaagcc
     9001 gcagtcggcc tacgtttata tttgctccaa cgaaaccatc ggtggcgttc gttttccgcg
     9061 ggaaccggaa accggcggaa tccccctggt gtgcgatgcc tcctcggagt tgctcagtcg
     9121 tcccgtggat atgtcgcgtt atggaatcct gtacgcttgc gcgcagaaga acctcgggcc
     9181 cgcgggcgtg acggtcgtta tcatccgtaa ggactttgtc gaagcctgcc cggatgacct
     9241 cccctcgatg ctaagttaca agaaattcgc cgcggagaag tcgctgctca atacgccgcc
     9301 ggttttcgcc gtgtatatgg tcaagctggt gaccgactgg ctaatccgcg aatttgggga
     9361 tctgaagcgg gtggcagact ataacgagga aaaggccaat ctgatttatg acgtcattga
     9421 ccgctgcggg gggttctatc aggtccacgc cgcaccggga agccgttcca cgatgaacat
     9481 cgctttccgc cttcccaccc aggaattgga aaaggaattc ctcaaacagg ccgaagcccg
     9541 caacctctac aatttgcagg ggcaccggtc ggtgggtggt tgtcgggcat ccatttacaa
     9601 cgcgatgccg cgggaagggg tggtcgccct ccgcgatttc atgctcgaat tcttcgaaga
     9661 gaatcgtcat cggctcgcgt gatttggccc gacagcttcc gggctgggaa aacattgggc
     9721 accgggatcg tccacgattc cggaaaggat aaaatcttct caaggaaggt cgccacatgc
     9781 acgaagattt gttgggcaaa ctgacgccgg cccagcgaga ggcagtaacc catgtcgagg
     9841 ggccgctcct catcttggcc ggaccaggca gcggcaaaac gcgcgtcgta accttccgca
     9901 tcgcctattt gttgcggctg ggaatccccg acaaaaacat cctggggcta acctttacca
     9961 acaaagccgc cgacgaaatg cgctcccgcg tggaggccct tgttcccggt gcccgcgtgt
    10021 ggctcgggac gtttcaccgc ttcgccgcgc aaacgctgcg tcagtatgcc caatttgtcg
    10081 gcctggaacc gaatttcacc atttacgaca aggaaaatag tcttcgcgct ctcaaggccg
    10141 tcatcaacga cgccaatatt gacctgggaa gctattccgt ggaatcggtg gcccacgcca
    10201 tcagcacggc caagagtgcc ctcatcttac caacggacta cctgccgcga gaaggttcgc
    10261 ctctcggtga gctcgtgcga cggatctatc cgctttatca acagcggctc atccagtcca
    10321 atgccgtgga ctttgacgac ctgttgatgc atctggccat cctccttcgt cagcatccgg
    10381 aaatccgtaa gcg
//
LOCUS       DAOSUT010000143        10388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3583_length_10388_cov_8.756218, whole genome shotgun sequence.
ACCESSION   DAOSUT010000143 DAOSUT010000000
VERSION     DAOSUT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_10388_cov_8.756218"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1489
                     /locus_tag="PLD05_12205"
     CDS             239..1489
                     /locus_tag="PLD05_12205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP78249.1"
                     /translation="MSFSVLLHDSWETLEQWADQWDRWAHGKPFRSWDWVTTWCSIYG
                     VAEPSCRLHVLSVHQGDGALGFAPLFMSWHPFWGWTIRFLGSGEICGDDVGWVAPGEL
                     QETVGRAIARALHHGTANACSMVTGGTNGSRTSIRIPTCDQVDLDGVRLSDRGLQGFL
                     QELQGLGWAIDIRPIEHCWQIPLPKTWEEFLQNLPRSFMRKKIRKAQQRYLSPGGVHV
                     HTVFGTIPWEEAWRHLINLHQARRLQLGDGGAFASGRFLRFHQEILSRWLPSGKARLV
                     WLEWQGQAIAAEYLLISEETVRAYQSGMNPQFGHMSPGFLANLAVIQQAITEGYRVFD
                     FLRGDEPYKADWGARPLEMVRIRAVPPRAVPQMRLRLWQTAKACVNVFRKKNHRECCP
                     RLVEYDAPSGEVLSPTAIMDGTTC"
     gene            1483..2514
                     /locus_tag="PLD05_12210"
     CDS             1483..2514
                     /locus_tag="PLD05_12210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOP78250.1"
                     /translation="MLNVRLALLTLYYHATWPLRRWWIRRWQKQACVPMSVLLYHRIA
                     SDCPVSWVTSLSAFRQHLAWLRRHAEVVSLHEIQARLISGNRESRPCVAITFDDGYAD
                     NLREAVPLLLEARMPFTFFVTVTNLLQERRFAHDLEQGQSWRLVSPRELRELADSGVE
                     VGSHGLTHADYSLLNIDELVREINDSKHILEDLAGRPVRALAIPFGQPRQMTPEVFQI
                     AYEAGYTCVCSAYGGYNIPGRHRFHIRRFHGDDPLIRLINQVTIDPRLVWQTQRDSVW
                     EAQSERPGSLMRGNEDAIVAPCTLLSQDGEQRPAVGETSARLPTGTAPLPEAGAATSP
                     DKVLLGGNL"
     gene            2511..5393
                     /locus_tag="PLD05_12215"
     CDS             2511..5393
                     /locus_tag="PLD05_12215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP78251.1"
                     /translation="MTVASCDDTLRQGDVVVATPGDGGAQGPETEQHSLSPWAAGVVV
                     VLVASVLQRAIGFVRAAWFCRWLDAEQLGTWDLAFSFLMTATPLVVLALPGCLGRYVG
                     HFQERGMLGPFLRKVAFLQGGLSAFAGVVMVFLAEPIAKLIFGSSGFASYVYLLAAGL
                     VVVAWSNYLYELTTALRLSRWIATLQLINSVLFAVFGIGLLWIGPRSAESVIIAYLVA
                     CAITIVCAYFVVRPWLLESHSNVTSQIGHVFWPKVLWYSLGLWATGTVGNLFAVVDRY
                     MIVHFSPLGVNALGQVGDYHAARLVPVLLVSLAGLVATLSLPHLSHSWERNRRERCGE
                     QLLFLVKVSGLLLMVVSTGVLIGRSVIFDSLLAGKYPAAEEILPLTLLTCCWFSLFLL
                     LENYVLCCEKAHWAAIAMVAGLLANVILNICWLPLWGLWGAIAATAVANALALGVLGL
                     VSYRLGLPVRLPLLWIILMPLGLLGGPVVAAILTVTMILLATRTRLLFSPSEKREFVH
                     FWKQLTGLHQALRSFLVGGRWHRQNPPKALPLDTDARTSCLEPAFVGKAGRAWPNPFF
                     RRKRHPPLGDRGPLRVMFVITSMPVGGAERLLVEIIRRLDRRIIQPELCCLKTLGPLG
                     EELAQEVPAFSQLLSHKYDLRVLPRLYLLMRRRHIDAVITVGTGGDKMFWGRLAAFLA
                     GVPVIASALHSTGLPDRVEWLNRLLTPLTDAFIAVAQPHAEYLVAHEGCPRDRTWIIA
                     NGVDTDRFSPRPPSPERRRELGLPPGCPVVSIVAALRPEKNHELFLQAAAIIHKAIPE
                     AHFVIVGDGPRREMLEQMATQLGLQEVVHFLGTRHDIPDILACSDVVVLSSHMEANPL
                     AVLEAMACGKPVVATRVGSLPTNVVDGLTGFLVPPGDAVALADRCVLLLRSARLREEM
                     GRAGRRHVLNRASLTHTVKGYEDLIVKLYARKCGSRIPILGKGCPS"
     assembly_gap    5605..5704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5707..6549)
                     /locus_tag="PLD05_12220"
     CDS             complement(5707..6549)
                     /locus_tag="PLD05_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP78252.1"
                     /translation="MKLTRRDCLRLSGTAGLLLACGQAPLSAFERKKIPVGVQLYSVR
                     QDAAKDLPGALKAIKEMGYDGVEFAGYYGHDAKSIRKWLDDNGLKCCGTHTGWDTLQP
                     DKLAETIEFNQIIGNKYLICPWLNPAEFAASREKTIEFARRFNELAEKVKEKGMLVGY
                     HAHGGDFKKLNGETAWEIFFSHTVPEVVMQMDIGNCLGGGGDPYAILEKFPGRSKTIH
                     LKEHGGPKGAVIGEGEVKWDWVFNFCETKGGTEWYIVEQESYAVPPLEAIRKCRENLR
                     KMGI"
     gene            6990..8324
                     /locus_tag="PLD05_12225"
     CDS             6990..8324
                     /locus_tag="PLD05_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78253.1"
                     /translation="MGTTGRFSRRDFLAAGTGVAVATYVSARALGLEPGQPSANNKLN
                     VAAVGVGGMGKHDIAQAARTENVVAICDVDRRFAEQTAAQYPNARVYVDFRKMLEEQK
                     DIDAVMIATPDHTHAVITAMALKLGKHVFCQKPLTHSVSEALKIRELAAQAKTATQMG
                     NQGQASEAARRICEYIWSGAIGKVREIHSWSNRRPDISPRGIPRPKDTPPVPDYLNWD
                     LWLGPAPERPYHPAYHPFAWRGWWDFGTGVLGDIGCHNLSAAFKALKLQWPISVEACS
                     THWSAPPEITKETAPLASIVTYVFPAEGDRPEITLRWYDGGMMPPIPKELGDRNIFDG
                     DGTLIVGDKGMLWGDRLLPESLAQEIGDPPKMLPRSPGHYEEWIAACKGGPAAGSNFV
                     DHAAHLAAVVLMGNIAIRTQKKLFWDAEKLRFTNSEEANALLDPPYRNGWSL"
     gene            8437..>10388
                     /locus_tag="PLD05_12230"
     CDS             8437..>10388
                     /locus_tag="PLD05_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOP78254.1"
                     /translation="MRSFTRLLVTGIIGAVVGTWCASISQAAGSESTSELFPFVLSYD
                     VADTITNLSDWLERPAGKNGFIRVENGHFVNDRGRVRFWATNLCFEACFPTKEEAERV
                     ARRLASLGINCVRMHHMDNRHIWGKSPNKLTIDPEMLDKLDYLIYQLKQHGIYTNINL
                     HVSRQFGPAEGFPAVEGLPNYDKGIDNFEPKMIEYQKKYARDLLTHVNPYTGNAYVNE
                     PAVAMVEINNENAAFDEYRKGAFDHLPEPYATQLRKLWNSWLRKKYGGDDALRKAWNA
                     QREPLGEEILKLGNFFGNWQGVWNLQRDRLSEVTAEAIPNIREGQPGLRLRVIRNGEQ
                     SWIPQLSQGGFAVKKGQVYTLSFWMRADQSGRIDVNCMMNHDPWQRLGLSGQVEVGKE
                     WKEYRLSFVAEQDDANARVTFSQLRPGVYELTGVSLRPGGVMGLEQGQSLVDDSIPIV
                     PWRGPQMTPAARADFADFLWELERDYWWGMYRFLKDELKLKSVVSGTQLSYSPIHIQA
                     GLDYIDAHSYWQHPVFPGRPWDPENWYVRNVALVNSGGGTLSGLANRRVEGFAFTVSE
                     YNHPAPNEYAAEGFPMIAAFGAFQDWDGIFSFTYSHSRDFEPRKITGFFDIKSEVGKL
                     VHMPACAALFLRGDVQPAKEAVLVALS"
BASE COUNT         2034 a         2827 c         2964 g         2463 t
ORIGIN      
        1 ccccctctcc ctccgggaga gggttggggt gagggcaagc gaacgtcgga gtcgattgag
       61 aaaaccgtga gcgattcagg ccaccctcac ccccggcccc tctcccgccg aacgggcgag
      121 gggaggttgt gggatagtct ctaaatcgaa agttgctttg ccgcgaatca cggcccatgt
      181 tttctcaagg gaagcccata tctgcgacgg ctttcgcaaa cggcagaaaa gccaccatat
      241 gtccttctcc gtcctcttgc acgactcttg ggaaacgctt gaacagtggg ccgatcaatg
      301 ggatcgctgg gcccatggca agccctttcg ctcatgggac tgggtgacca cctggtgctc
      361 gatctatggg gtggcagaac caagctgtcg cctacatgtg ctatccgtac accagggaga
      421 tggtgccctc ggttttgcac ccctctttat gtcgtggcat ccgttctggg gatggacgat
      481 ccgttttctc ggctcaggcg aaatttgcgg cgatgacgtg ggctgggtcg cccctggaga
      541 gctccaggaa acggttgggc gtgctattgc tcgggccctt catcatggta cggccaacgc
      601 ttgctcgatg gtcaccgggg gaaccaatgg atctcgaact tcaataagga tccctacgtg
      661 cgatcaggtc gatctcgatg gcgttagact ctccgaccga gggcttcaag ggtttttgca
      721 ggagttacag ggactcggct gggcgattga catccggccc atcgagcact gctggcagat
      781 tcctttaccc aagacatggg aggagttcct gcaaaacctg ccgcgttctt tcatgcggaa
      841 gaagattcga aaagcccagc aacggtacct tagccccggc ggggtccacg ttcacacggt
      901 ctttggcact ataccgtggg aagaggcctg gcgtcatctg atcaatcttc atcaagcgcg
      961 gcgcttgcag ttgggtgatg gtggggcctt tgcctcgggg cgttttctga gatttcatca
     1021 agagatactc agtcgctggc tcccctcggg caaggcccgc ttggtctggt tggaatggca
     1081 aggccaggcc atcgcggcgg aatacctcct gatatcggag gagactgtac gtgcctatca
     1141 atcggggatg aatcctcagt ttgggcacat gtcgccgggt tttctggcaa atcttgcggt
     1201 gattcaacag gccatcaccg aaggctaccg agtattcgac ttcctccggg gcgatgaacc
     1261 atataaggct gactggggtg ctcggcccct cgagatggtc cggatacgcg ccgttccccc
     1321 tcgcgcagtg ccgcaaatga gactgcggct gtggcagacg gcaaaagcct gcgtcaacgt
     1381 gtttcgcaag aagaaccatc gggaatgctg cccgcgctta gtcgagtacg atgcaccctc
     1441 gggcgaagtc ctttctccta ccgcgataat ggacggaaca acatgctgaa tgtgcgactg
     1501 gccctgctca ctttgtatta tcacgccaca tggccacttc gtcggtggtg gatccggcga
     1561 tggcaaaagc aggcctgcgt cccaatgtcg gtcttgttgt atcaccgtat tgcgagcgat
     1621 tgccccgtgt cttgggtgac ctcactatcg gcctttcggc agcacctggc atggcttcgc
     1681 cgtcatgcgg aagtcgtttc tctccatgaa attcaagccc gtttgatttc tggcaatagg
     1741 gaatcgcgtc cctgtgtggc catcaccttc gacgacggct atgccgataa cctgagggaa
     1801 gccgtacccc tccttcttga agcgagaatg ccttttacgt tctttgtgac ggtgaccaat
     1861 ctcctccagg aacggcgctt tgctcacgat ttggagcagg gacagagctg gcgtttggta
     1921 agcccgcgcg agcttcggga gttggcagat tctggtgtag aggtcggttc ccatggattg
     1981 acccatgctg attattccct tttgaacatc gacgagcttg tccgggaaat taacgatagc
     2041 aaacacattt tggaagattt ggcgggacgg ccggttcggg ccctggcgat tcccttcggc
     2101 cagccgcggc aaatgacgcc cgaggttttc caaatcgcct acgaagcggg atacacgtgc
     2161 gtttgctcgg cctatggtgg atacaacata ccggggcgcc accgattcca tatccgccgg
     2221 ttccacggtg atgatccttt gattcggttg atcaaccagg tcaccattga tcctcgtctc
     2281 gtctggcaaa cacaacgcga ttccgtttgg gaggcacaaa gcgagaggcc cggatcgctc
     2341 atgcgcggca acgaagacgc cattgttgcc ccatgcactt tgttatcaca agacggcgag
     2401 cagcggccag ctgtcggcga gacttcggcc agattgccga caggaactgc accactcccg
     2461 gaagccggcg cggccacttc gccggataag gtcctgctgg gaggcaacct atgacggtag
     2521 catcttgtga tgacacattg cggcaaggtg acgtcgttgt cgcaacacct ggcgatgggg
     2581 gcgctcaagg gcctgaaacc gagcaacaca gtctttcgcc ctgggccgcc ggggtggttg
     2641 tcgtcttggt ggcttctgtt cttcaaaggg cgattggttt tgttcgggcg gcttggttct
     2701 gtcgctggct cgacgctgaa caactcggca catgggacct cgcctttagt ttcctgatga
     2761 cagccacccc gctggtggtc ctcgcattac ccggctgttt gggacggtat gtcggacact
     2821 ttcaagagag agggatgctg gggcctttcc ttcgaaaagt ggcttttctt caaggaggat
     2881 tatccgcttt tgccggcgtt gtgatggttt tcctagccga acccattgcc aagctcattt
     2941 tcggctcatc cggctttgcc tcctatgtgt acctcttagc ggcgggactt gttgtcgttg
     3001 cctggtcaaa ttacctgtat gaattgacca ccgcacttcg tctcagtcgg tggattgcga
     3061 cattacagct tattaatagt gtcctttttg ccgtctttgg tatcgggctg ctctggatag
     3121 gaccccggtc tgcggagtcc gtcatcattg cctatctggt tgcctgcgcc atcacgatcg
     3181 tgtgtgcgta ttttgttgtc cggccatggt tattggaaag tcattccaac gtcacatcac
     3241 aaatagggca cgttttttgg ccaaaagtcc tttggtactc gctcggtctt tgggccaccg
     3301 ggacggtggg caacctgttt gccgttgtgg atcggtacat gatcgtccac ttttcgcctc
     3361 tcggggtgaa cgccctaggg caagtcggtg attaccacgc ggcgcgactg gttcccgttc
     3421 ttctcgtttc ccttgcggga ctggtggcca cgctttcctt gcctcatctt agtcatagct
     3481 gggaacgcaa tcgccgtgaa agatgtggcg agcaattgct gtttctcgtg aaggtttccg
     3541 ggcttctttt gatggtggta agcaccgggg tgctcatcgg tcgttcggtg atttttgata
     3601 gcctactcgc cggaaaatac cctgctgccg aggagatcct tccattgacg cttctcacct
     3661 gttgctggtt ctccttgttc cttctgttgg agaactacgt cctttgctgc gaaaaggccc
     3721 actgggcggc cattgccatg gtggcgggtt tgctggcgaa tgttattctc aacatctgtt
     3781 ggctaccact ttggggcctg tggggagcca ttgcggccac ggccgtcgcc aatgctctgg
     3841 ccctgggagt tttggggttg gttagttacc gattagggct cccggtgcga ttgcccctcc
     3901 tctggatcat cctcatgccc cttggactgc tcggtggacc cgtggtggca gcaattctca
     3961 ccgtgacaat gatcctgctc gctacgcgga cacgactcct tttctcccca agcgagaaac
     4021 gcgagttcgt gcatttttgg aaacaactaa cggggctgca tcaagctctt cgcagtttcc
     4081 tagtaggtgg gcgatggcat cggcaaaacc ctccaaaggc gttgcctctc gacaccgacg
     4141 cgcgcacctc ctgcttggaa ccggcctttg tggggaaagc aggccgggcc tggcctaacc
     4201 catttttccg aagaaaaagg catccaccgc tcggggaccg cggaccactt cgtgttatgt
     4261 tcgtgatcac ctcgatgccg gtcggtggag ccgaacggct gcttgtggag attattcgcc
     4321 gccttgatcg ccgcattatc cagcccgaat tgtgttgttt gaagaccctt ggtccgctag
     4381 gtgaggagct cgcccaggaa gtcccggcgt tttcacagct tctcagtcac aagtacgatt
     4441 tgcgcgtcct gccgcggctt tatctactca tgcgaagacg gcacatcgat gccgtcatta
     4501 cggttggaac aggtggtgat aagatgtttt ggggacggct ggccgcgttc ttggccggtg
     4561 tcccggtgat agcgtcagca cttcattcga cgggcttgcc cgatcgggtc gaatggctga
     4621 accgtctcct tactccgctg accgatgcat tcatcgcggt tgcccaaccc catgccgagt
     4681 atctcgttgc ccatgagggc tgcccgcgcg accggacttg gatcatcgct aatggagtcg
     4741 ataccgaccg gttttcgccg cgcccaccct ccccggagcg gcgtcgagag ctagggttac
     4801 ccccaggatg tccggttgtg tcgatcgtag ctgccctgcg tccggaaaag aaccacgagc
     4861 tttttctcca ggcggccgca attatccaca aagcaattcc tgaggcccat tttgtcattg
     4921 tgggcgacgg gcctcgccgg gaaatgctgg agcaaatggc aactcagctt gggctgcaag
     4981 aggtcgtcca ttttttgggg acccgccacg acattccgga catcttggca tgttccgacg
     5041 tggttgtgct ttcttcgcac atggaagcca atccactggc cgttctggaa gcgatggcct
     5101 gcggcaaacc ggttgtcgcc acgcgggttg ggtcgctccc cacaaatgtg gtagacggcc
     5161 tgaccgggtt tctcgtcccc ccgggcgatg cggtggcgct tgccgaccgg tgcgtccttc
     5221 tgctgaggag tgcccgatta cgcgaggaga tgggacgtgc gggccgccgg cacgtcctaa
     5281 accgggcttc gctcacgcat accgtaaaag gctacgagga cctcatcgta aagctgtatg
     5341 cgcgcaaatg cggctctcgt ataccaattt tggggaaagg atgtccatca taaggtgggg
     5401 tagtggatga ggccggcccg cggttaagac aaggcctttc ggcgaagagt cgcggcgccc
     5461 ccgggagcat tcatcctgat gcgccgcatt agcgttggaa ctaattgcac gataatcgcg
     5521 tccaatgggt gatgagtctt gcgtatttat gccatatcca gtagagttcg tattttcgtt
     5581 attttgctat tgattccaag tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnncgttaa atccccatct tgcgaaggtt ctcgcggcac ttgcggatgg cctccagagg
     5761 tggcacggcg tagctctcct gttccacgat gtaccactcg gttccaccct tggtctcgca
     5821 gaagttgaac acccagtccc atttgacctc gccttcaccg ataaccgccc cctttggccc
     5881 gccgtgttcc ttaaggtgaa tcgtctttga tcgtcccggg aatttttcca agattgcgta
     5941 ggggtctcct ccaccgccca agcaattccc gatatccatt tgcatgacca cctcaggaac
     6001 cgtgtggctg aaaaagatct cccaggccgt ttccccgttt agcttcttga agtcgccccc
     6061 atgcgcatga tagccaacca gcattccctt ttccttgacc ttctccgcaa gttcattgaa
     6121 tcgccgggca aattcaatcg tcttctctcg actggctgcg aactcggccg ggttaagcca
     6181 cggacaaatc aggtacttgt tgccgatgat ctgattgaat tcgattgtct cggccaattt
     6241 atcgggctga agggtgtccc aaccggtatg ggtaccacag cacttcaaac cattgtcgtc
     6301 caaccatttg cgaatggact tcgcatcgtg tccgtagtag ccggcgaatt ccacgccgtc
     6361 gtaacccatc tctttgatgg cctttaatgc acccggcagg tcttttgcgg cgtcctgacg
     6421 gacagaatag agttgaacgc ccaccggaat ctttttccgc tcaaaggcag acaggggcgc
     6481 ctggccacat gcgagtagca atcccgccgt accggacaac cgcaaacaat cgcgtcgtgt
     6541 cagtttcatt gtttctcctc ctcacaagtt gctaaccaag actcgccttt cggcttctag
     6601 agccacaacg actgatcatg ataatgtgtc ggaccggccc taagcaaatg gggtcgataa
     6661 cggactgcca gagcgccccc tacccatggt ggaagaactt gccccctctc agaaagcgca
     6721 tcgttgttaa gtgcggccac gacacggtgt agttctccat aaggaggtcg ggcgtcggtt
     6781 ggcatcggcc cttgtggtta cccaattatt gaatctgcag agatgcgccg cggcgtgcgt
     6841 ttctggggcg cgggcgaaag atatctcggg agtcagctgc gcaacctggc gggtcgatat
     6901 accttgcttt catttcgagt gcgcatcatt tacaatgccg tcgcaacggg aattggtcag
     6961 taagagttgc ttgattggga gggtttatca tggggacaac cggtcgcttt tctcgacggg
     7021 attttttggc cgcggggacg ggagtggctg ttgccaccta tgtttccgcc cgggccttgg
     7081 gccttgaacc aggacagcct tctgccaaca acaagctgaa cgtggcggcg gtcggtgtgg
     7141 gcgggatggg caaacacgat attgcccagg cggccaggac ggaaaatgtc gtagccatct
     7201 gcgatgtcga tcgtcgcttc gcggagcaaa cggcggcaca gtatccaaac gctcgcgtgt
     7261 atgtcgattt tcgcaagatg ctggaagagc agaaggatat cgatgccgtg atgatcgcaa
     7321 cgcccgatca tacgcatgcc gttattacgg caatggccct caaactggga aaacacgttt
     7381 tttgtcagaa gccactcacg cacagcgtaa gtgaagcact caaaatcagg gaactggcgg
     7441 cccaagccaa aacggccacg cagatgggta atcagggtca ggcttcggaa gccgcccgac
     7501 gcatttgcga atatatctgg tccggcgcca tcgggaaggt tcgtgaaatc cattcttggt
     7561 cgaatcgccg tcccgatatc tcgccccgcg ggattcctcg tccaaaggac acaccccctg
     7621 tgcccgatta cctgaattgg gatttgtggt tgggcccggc gcccgaacgg ccgtatcatc
     7681 cagcgtatca cccttttgct tggcgcggtt ggtgggactt tggcaccggc gttcttgggg
     7741 acatcggatg ccacaatctt tcggcggcgt tcaaggccct gaagctccag tggcccatct
     7801 ctgtggaggc ctgctcgacc cattggagcg cacctccgga gatcacaaaa gaaacggcac
     7861 ccttggcgtc gattgtcacg tatgtcttcc ccgcagaagg agatcgcccg gaaatcacac
     7921 tccgatggta cgacgggggc atgatgccgc cgattcctaa ggaacttggg gatcgcaata
     7981 tcttcgacgg cgacggtacc ttgattgtcg gcgacaaggg catgctctgg ggcgatcgcc
     8041 tcttgccgga gtccctcgcc caggaaattg gtgatccccc taaaatgctt ccgcggtccc
     8101 ccggtcacta tgaggaatgg attgcggcct gcaagggtgg tccggcggcg gggagtaact
     8161 ttgtcgatca tgccgcacat ttggcagcgg tggtccttat ggggaacatc gccattcgca
     8221 cccaaaagaa gcttttctgg gatgctgaga aattgcgatt cacgaactcc gaggaagcca
     8281 acgcgttgct cgatccaccc tatcgcaacg gttggtcgtt gtgatctata atcatcactg
     8341 agttcgtccg agcctcgcgc agggagctga gagtgcgcga gtcgttttac gtccttccct
     8401 tactcaaatc accaggatac gaaaggaggt ctcgccgtga ggtctttcac gcgcttactg
     8461 gttaccggga tcatcggcgc tgtggtggga acttggtgcg cttcgattag tcaggcagca
     8521 gggtctgaga gtacatcgga gcttttcccc tttgtgctaa gttacgatgt ggccgatacg
     8581 attacgaacc tttctgattg gttggagcgt cccgccggaa agaatggttt catccgggta
     8641 gaaaatggtc attttgtgaa tgaccgtggt cgcgttcgct tttgggccac gaacctttgc
     8701 tttgaggcat gctttccgac caaagaggaa gcggaacgag ttgcgcggcg tcttgccagc
     8761 ttgggaatca attgtgtgcg aatgcaccac atggataatc ggcatatttg ggggaaaagc
     8821 cctaacaagt tgaccattga cccggagatg ctcgataaac tggactatct catctatcag
     8881 ctcaagcaac acggcattta taccaatatt aacctccatg tctctcggca gtttggacca
     8941 gcggaggggt ttcctgcggt ggagggcctg ccgaattacg acaaggggat cgacaatttt
     9001 gagccgaaga tgatcgagta tcaaaagaaa tatgcccgcg acctcctcac tcatgtgaac
     9061 ccctataccg gtaacgccta cgtcaatgaa ccggccgttg cgatggttga aatcaacaac
     9121 gaaaacgccg cgttcgacga gtaccgtaag ggggcattcg atcatcttcc ggaaccatac
     9181 gccacgcaat tgcgcaagct gtggaatagc tggttgagga aaaagtatgg tggcgatgat
     9241 gctcttcgca aagcttggaa tgctcagcgc gaaccattgg gcgaggaaat tctgaaattg
     9301 ggcaatttct ttggtaactg gcaaggcgtg tggaatctcc agcgcgatcg cctttccgag
     9361 gtgacagcgg aggctattcc caacattcgt gagggacaac ctggcctgcg tttaagggtt
     9421 attcgcaatg gtgagcaatc ctggattccc caacttagcc agggtggctt tgccgtgaaa
     9481 aagggacagg tgtacacgct cagcttttgg atgcgggcag atcaatcggg acgcatcgat
     9541 gtcaactgca tgatgaatca tgatccctgg caacggctgg gtttgtcagg tcaggtcgaa
     9601 gttggaaagg agtggaaaga gtatcgattg agcttcgtcg cggaacagga cgatgccaat
     9661 gcccgcgtga cctttagtca acttcggccg ggcgtctatg aactgaccgg tgtctcgttg
     9721 cggccagggg gcgtaatggg actggagcag gggcaatcgc tggtcgacga ctcgatcccc
     9781 atcgttcctt ggagaggccc tcagatgacg ccggcagcgc gggccgattt tgccgacttc
     9841 ttgtgggagc tagaacgtga ttactggtgg ggcatgtatc ggtttctaaa ggatgagttg
     9901 aagttgaagt ccgtggtttc gggcacgcag ctttcgtata gccccatcca catccaagca
     9961 ggcctcgatt acatcgacgc ccactcttac tggcaacatc ctgtcttccc cggacggccg
    10021 tgggatccgg aaaactggta cgtccgtaat gtggccttgg tcaacagtgg cggaggaaca
    10081 ctgtcagggc ttgctaatcg ccgtgtggaa gggtttgctt ttaccgtaag cgaatacaac
    10141 catcccgcgc ccaatgaata tgccgcggag ggctttccga tgatcgccgc ctttggggcc
    10201 ttccaggact gggacgggat tttcagtttt acatacagcc acagtcgcga ttttgaaccg
    10261 cggaagatca caggcttttt cgatatcaag agcgaggtcg gcaaactcgt ccacatgccg
    10321 gcctgtgccg cgttgttcct tcggggtgat gtgcagcccg ccaaagaggc cgtgctggtg
    10381 gcgttgtc
//
LOCUS       DAOSUT010000144        10366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3591_length_10366_cov_9.427020, whole genome shotgun sequence.
ACCESSION   DAOSUT010000144 DAOSUT010000000
VERSION     DAOSUT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3591_length_10366_cov_9.427020"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PLD05_12235"
     CDS             complement(<1..113)
                     /locus_tag="PLD05_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78255.1"
                     /translation="MKARRQQENHRPQVQLFTDGACSGNPGPGGWAFILYHP"
     gene            complement(186..1961)
                     /locus_tag="PLD05_12240"
     CDS             complement(186..1961)
                     /locus_tag="PLD05_12240"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78256.1"
                     /translation="MPQRRLNRALVETSLEVKCLFLFGVFLVLVIAVSVLLYWRVTGE
                     VVTRQNPDTARLLVDQVMLTKHYAAMEPNKDFVDYLNGVIKSRSQQGYRWRFVRPPGR
                     LMNQSEDTGPPLDAMESELIQRFMAMTEEERSHPDAQLYAERLVDGGNTYQYYQPIWA
                     EQSCVPCHIALEGAGYSPTSGGIMTPIREGDLMAILQVTIPNGPTQAAVMKYWSMLLA
                     VAIVTAFLAMIAFYITIRYVIVRPLRHLRAVSEAITEGNTALRAHIHTRDEFELLANA
                     FNRMLSHLVAVQDELRQVNAELDAKLDEVAQLNMQLYEMNRVKSDFMATMSHELRTPL
                     NSILGFSEVLASIESLDEKQRRYVQNIQKSGRILLEMINNVLDLAKMEAGRMEIRPTE
                     FSIEQVVQAQCDMARPLTEKKNLQLTVDIQPNLPPMFQDQNRVAQILNNLLSNAIKFT
                     PEGGAIRVIVRRDNNGDLILRVADTGVGIAEEDRQIIFEKFRQGRTAMPDGDPLKREY
                     PGSGLGLSIVKELCRLLGGEVGVESELGRGSVFTVRLPWRLEIAEEGDQPPYFRPINA
                     EEMRFDRDRVSKRLRPTPASEKGPR"
     gene            complement(2150..4441)
                     /gene="priA"
                     /locus_tag="PLD05_12245"
     CDS             complement(2150..4441)
                     /gene="priA"
                     /locus_tag="PLD05_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOP78257.1"
                     /translation="MVLKQLTLFDDFFASEPQKPSTVAMVASVVFGQMPKGEFDYLVP
                     KHLQGVLEPGQRVRVPLGKSNRLQIGYCVSVTHKSCVTSELKAIHSIVDEQRLLSPAM
                     LQVTRWIAEHYLCDWGQVLETVLPKCVRENVGLRKVFKYRLAHPKDGLQHAGRLTAKQ
                     QRVLEILASADGPLTAEDLVAKAGCSSAVLGNLVKRGLIEREISHVRDKTISYDRPPR
                     AESLTLNSEQRAALQTIVDAIRSGTHKTILIHGVTGSGKTEVYIQAIEEVVRRGCQAI
                     VLVPEISLTPQTVERFRSRFHNVAVLHSHLTDAERAAQWEWIASGAVEVVVGARSAVF
                     SPTPRLGLIILDEEHESSFKQESAPRYHAREVAEQRARIEGIPLVLGSATPSLESWYR
                     AQQGEAILVQLPRRIANLPLPDVKTIDLREPKRARETRGVITRQLHAAIEEVLKDGGQ
                     VILLLNRRGFATHVQCPSCGVVLRCPHCDISLTHHKIANRVVCHYCDYEVETPRTCPQ
                     CDSPRIRFSGTGTERLEAEIKARFPHATCLRMDADSMRSRLAYERAFAEFRRGRIQIL
                     VGTQIIAKGLDFPNVRLVGVVNADTALHFPDFRASERTFQLVTQVAGRTGRGPHGGQV
                     LVQAFTPEHPAIQAAIRHDYETFAQQELKVREAFGYPPFWRLARIVVSSNSSETAEAC
                     SREFAASVHTQAEVAGLTDKIRIVGPAPAPLAKLRGKYRYHLQVMARDYPQLLSVIST
                     AQKTCRAPSGVTWTIDVDPIDML"
     gene            complement(4535..6925)
                     /locus_tag="PLD05_12250"
     CDS             complement(4535..6925)
                     /locus_tag="PLD05_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78258.1"
                     /translation="MLIVLVTFGITTVAVGESAGDGQVIFAVIRGQQEGKNLLAEGTW
                     GKYEQGFEEHEGVIVCENKDKPGYRAGAVQSIVLNQKEPRPLVAVAESAAQDVDGARD
                     NNYSVYLDIEYNDGTPLWGQAAAFDVGTHDWQERRVVIFPEKPIRRLSYYLLFRGHRG
                     KAMFRNPRLYEISTPGTALFDGIPCTKAVGDYRGLVVRDVAKNSDFVALQQSALGIEC
                     VWTVTEKDNVRTYDLILRSRSDEDRALTVAFVEPVKCTSSGDKPILWLQSPRRSEAVS
                     ATREYATTVRTRAGATQRLGRWPLAAVATDTQGYGVGIDMIYPAVFRVGYNAAYEQLF
                     VAYDIALTKEKPDAHLRFVVFTFSPHGGFRAAWARYIEIFPDYFSSRISKHGQWMPFA
                     KISQVEHWEDFGFRFKEGNNETQWDDAHDILTFRYTEPMTWWMPMAPEIPRTYEAAVA
                     EAQRLAQQGRPQAKAWLSSSFRDENGRIPLRLLDTPWCNGAVWSMNSLPQIRGEITDF
                     KLKWNPDIRQALYGPNRKGDLDGEYIDSSEGYVTDELDFRRDHFVGERPLTYDASSFQ
                     PAVFRGLIVFEYVRAIAEDVHAMGKFMMANGTPSHLCWLVPLLDVMGTETDWNPNGQW
                     RPMSDSELLFRRALCGKKPYCFLMNTNFDRFDYPLVEKYMKRCVAYGMFPGFFSPNAS
                     GGHYFTRPELYNRDRPLFKKYVPLCQRLSEAGWEPITRAQCNVPNVYVERFGDRYFTV
                     FNEASQEATAEVTFDADLTLPATVQELVTAKTLTVVNRKVTIRLQGEDVAVLDIGQ"
     gene            complement(7042..>10366)
                     /locus_tag="PLD05_12255"
     CDS             complement(7042..>10366)
                     /locus_tag="PLD05_12255"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOP78259.1"
                     /translation="LFLLTVCLPGAWAFTEPLATRVGTAEVQVSDAQGRRLFYLDPAH
                     LEALGQTGGRIVGGPPQVVSDSENLKGLRFDGNTWIVFQEASHLDVPQLTIEICFKGQ
                     FSAPARYNPCLIAQRESGDHRQTRWSVHIMGDYSAIALWNGKSVAVFRSPAGALKPEV
                     WYHLVIVTQKSGTTVYLDGVPCITDSPSWTINIQEKGRPLTLAASQPSGHEAFLGALA
                     NVVIYDTAWTAEQVAARTDELGFRDERLALAQQYQEILKRQAEEQARLADLREKRRME
                     LMNDPALVTRGQPTVYEAERLECIDIPIGGIGVGAIHMNGRAERHAWQIFGSVAYRQI
                     PESFFAIGVSTPTRSIVRTLQTTSVAGIPPMQRLRLEAPYPFAVYRFQDEEVSCHVTL
                     TAFNPLIPLHARDSAIPCAVYRIGIHNPMNEPVTAAILGTQLNVVGYQADKPIDERRY
                     ERFGKNVNSVLRTDKGTWLCMTKQDAPDAGEMVLMVDSPRAKVLANWEDSESLTGLLR
                     APSQIESLQEVTTATSREGETVNGAVFSCVDIPPGETREVTYVLVWYLPGQPAGTGAW
                     GCKGRRYEAWWKNAQEVAAELEQRLPELEAASRMYVDSLYESNLPYWLIDRIASQVAI
                     LRSPTVFWCREGYFGGWEGCNVNEGCCHGNCNHVWHYAQAHARLFPEIAKQMREQEFT
                     YQKPDGAIPHRQPDSFPAFDGQCGAVLNSYREHLMSSDRSWLDSHWPAIRRAMDYVVQ
                     RWDPDQDGLLSGPQWNTLDGELGGNSSWLGSLYLAALTAAEKMARLEGDITSAEKYQR
                     IARLGAAKQDELLFNGRYYIQIPDPTPHEDYGEGCAIDQVLGQWWAHQLDLGWVYPPE
                     HVTSALRTLFALNFRGRMEGLPQLPRKFVADEDGGMQMFVWPEGAKKPSQIIKYASEV
                     MTGFEYAAAAAMIQAGLLKEGLTVCRAIALRYDGRKRVFPGAGNFNNWGYSGNPFGDD
                     ECGKFYARAMSVWSILLACQGFIYDGPDQRIGFIPVWQPEDHVSLFTAAEGWGVYRQW
                     RGDGEQNHSLSVRFGQLAVKTFEGATPAGTTPKTVQCVLEKREIPCVLRCDEGRFSCV
                     FEEPVVVHQAQSLDIRIAW"
BASE COUNT         2337 a         3006 c         2685 g         2338 t
ORIGIN      
        1 ggatgataaa gaataaacgc ccagccacca ggacctgggt ttccactaca ggccccatcg
       61 gtgaatagct gcacttgggg tcgatggttt tcctgttgtc ggcgagcctt catggcacct
      121 cacaattccc ctcgaaataa ccgggaatga acgagctgtt tttccaggat gacccccact
      181 ggccgtcacc gcggaccttt ctcactggcc ggcgtgggtc gcagtcgctt actgacacga
      241 tcacgatcga atcgcatctc ttcggcattt atgggacgaa aatacggtgg ttgatctccc
      301 tcttcggcga tctcaagccg ccaaggcaac cgcactgtaa agacgctccc tcgccccagc
      361 tcactttcca cacctacctc accgcctaat agccgacaaa gttcctttac gatggagagg
      421 cccaaccctg aaccggggta ttcccgtttg agaggatcgc cgtccggcat ggccgtccgc
      481 ccctggcgaa atttttcaaa gataatttga cgatcctctt cggcgatccc cacacctgta
      541 tcggcaacac gtaaaatcag gtcaccgtta ttatctcgac gcacaatcac gcgaatggca
      601 ccgccttctg gagtgaattt gatggcgttg gaaaggagat tgttgagaat ctgggcgacg
      661 cgattctgat cttggaacat cggcggtaaa ttgggttgga tatcgactgt caattgcaag
      721 ttcttctttt ctgttaatgg ccgtgccatg tcgcattgtg cctggacgac ttgctcgatg
      781 gaaaactcgg ttgggcgaat ctccatgcgc cctgcttcca tcttcgcaag gtccaggaca
      841 ttgttgatca tttccagaag gatacgaccc gacttttgaa tattctgcac gtaccgccgt
      901 tgtttctcat cgagagactc gatcgaggcc aggacttcgc tgaatcccaa aatgctatta
      961 agaggtgtcc tcagttcatg gctcatggtg gccatgaaat cgcttttcac gcgattcatt
     1021 tcgtacaatt gcatgtttaa ttgagctacc tcgtcgagct tcgcatccag ttcggcgttc
     1081 acctgacgga gctcgtcctg aaccgcgacc aagtgactta acatgcggtt gaaagcattt
     1141 gccaaaagct cgaattcatc tcgcgtgtga atatgcgccc gtaatgccgt attgccctca
     1201 gtgatcgcct cgcttacagc ccgcaggtgc cgcaacggtc gtacaatgac atagcggatt
     1261 gtgatataaa acgcgatcat tgctaaaaaa gccgtgacaa tcgcgacagc caggagcatg
     1321 ctccaatact tcatgacggc agcctgtgtc ggcccattgg ggatcgtgac ctgcaagatg
     1381 gccatcaggt ctccttcacg aatgggcgtc ataatgcctc cggacgttgg gctgtagccc
     1441 gcaccctcta aagcgatgtg gcagggaaca cacgactgtt ctgcccaaat cggctgatag
     1501 tattgatagg tgtttccgcc atcgactaat cgctcagcat agagttgggc atcagggtgc
     1561 gaccgttcct cctcagtcat ggccatgaac cgttgaatga gctcggattc catggcgtcc
     1621 aggggcggtc ctgtatcttc cgattgattc atcagtctgc ccggcggacg cacaaaacgc
     1681 caccgatacc cttgttgtga acggctcttg atgacgccgt tcaaatagtc tacgaaatct
     1741 ttgttcggct ccattgctgc atagtgcttg gtgagcatca cctggtccac aagcagccgt
     1801 gctgtatccg ggttttgccg cgtgaccact tccccagtga cccgccaata gaggagaaca
     1861 ctgacggcga tcacaagaac caaaaatacc ccaaaaagga agaggcattt cacctcgagg
     1921 ctggtttcga cgagagcacg attcaatcgt cgttggggca tcttcgttct gacccgagac
     1981 aacacattcc ttatggggct ggtaatcgcc gttgttgatc ggttttcttc atcttattca
     2041 attatgggag aatgcgaaac agtaaagcgt gggttgggac aggagcgaaa tcccaatcac
     2101 cacctcgatt agacgcgatt tcccgacgaa catgagcgtt caacgcctct caaagcatgt
     2161 cgatcgggtc aacgtcgatt gtccaggtta caccagatgg cgcacggcac gtcttttggg
     2221 ccgtgctaat gacgctcagc agttgtggat agtcacgtgc catgacttgg aggtggtacc
     2281 gatacttgcc gcggagcttt gccaaagggg ccggcgcggg gccgacgatt cgtattttgt
     2341 cggtcagacc ggctacctcg gcctgggtgt ggaccgacgc cgcgaattca cgcgagcatg
     2401 cctccgccgt ctcgctgcta ttcgaactga ccacaatgcg tgccaaccgc caaaacgggg
     2461 gatagccaaa ggcttcgcgg accttcagct cctgctgagc aaacgtctcg taatcatggc
     2521 ggatggccgc ttgaatggcg gggtgctcgg gcgtgaatgc ctgtaccaag acctgcccac
     2581 catgggggcc gcgaccagtt cggcctgcca cctgcgtgac cagttgaaag gtacgttcag
     2641 atgcgcggaa gtcgggaaaa tggagggccg tatccgcgtt gacgaccccc acaagccgga
     2701 cgtttgggaa atccagccct tttgcgatga tttgtgtgcc caccaaaatc tggatgcgtc
     2761 ctcggcgaaa ctcggcaaac gccctttcat acgccaggcg gcttctcatg gaatcggcgt
     2821 ccatccgcag acaggtcgca tggggaaagc gggctttgat ctccgcctca agccgttctg
     2881 tccccgttcc actaaaacgg attcgcggcg agtcgcattg agggcatgtc cgcggtgttt
     2941 ccacctcata gtcacaataa tgacaaacaa cgcgatttgc tatcttatgg tgggtgagac
     3001 taatatcgca gtgggggcaa cgcaacacca cgccgcagga gggacattgg acatgcgtgg
     3061 caaagccccg tctgttcagc aaaaggatga cctggcctcc gtccttgagt acttcttcga
     3121 tggccgcatg caactgacgg gtaatcaccc cgcgggtctc ccttgcccgt ttgggctccc
     3181 gcagatcaat cgtcttcacg tccggaaggg ggaggttggc gatccggcga gggagttgaa
     3241 caagaatcgc ctcaccctgc tgtgcccgat accaagattc caaagagggg gtcgccgatc
     3301 ctagcacgag gggaattccc tcgattctgg cacgctgttc tgccacttcc cgggcatgat
     3361 aacgaggagc tgactcctgc ttgaacgacg actcgtgttc ttcatcgagg ataatcagtc
     3421 ccaaccgcgg ggtcggcgaa aataccgcac ttcgtgcccc gaccacaacc tccaccgccc
     3481 ctgaggcgat ccattcccat tgtgcggccc gctcggcatc tgttaagtgg ctgtgaagga
     3541 ccgccacgtt gtggaagcga cttcgaaagc gttctaccgt ctggggtgtc aaactgatct
     3601 ccgggactag gacaatcgcc tgacaaccgc ggcgaacgac ctcctcaatc gcttgaatat
     3661 atacctcagt cttcccgctc cctgttaccc cgtggattag aatcgttttg tgcgtccccg
     3721 aacggatggc gtccacaatc gtctggagcg ctgcacgctg ttcgctgttg agcgtgagcg
     3781 attcggctcg aggcggcctg tcataagaga tggtcttgtc tcgtacgtga ctgatctccc
     3841 tttcgatcag cccccgtttc acaagattgc ctaacacggc cgaggaacaa ccggccttgg
     3901 caacaaggtc ttccgccgtc aatgggccgt cagcggatgc aaggatttct aggacccgct
     3961 gttgcttcgc cgttaaacgg ccggcgtgtt gtaatccatc tttcggatgg gctaaccggt
     4021 acttgaagac cttccgtaac cccacatttt cccggacaca ctttggcagg accgtctcca
     4081 agacctgccc ccagtcgcaa agataatgct cggcaatcca gcgggtcact tgcaacatgg
     4141 caggcgacaa gagtcgttgt tcatccacaa tggaatggat tgccttcagc tcggaggtga
     4201 cacaagactt gtgagtgacg gagacgcagt acccaatctg caaacggttg gacttgccca
     4261 agggtacccg cacccgctgt ccaggttcca acaccccctg caaatgtttc ggaacgagat
     4321 aatcaaactc tcctttgggc atttggccaa acacgacgct ggcaaccatt gccaccgtgc
     4381 ttggtttttg cggctccgag gcaaaaaagt cgtcgaaaag ggtcagttgt ttgaggacca
     4441 tgcaacgccg ttgtcagacc tccttaattg tgccgtgagt cgcccttgca gcctacgaac
     4501 gctcggggcg acatcctact gggccattgt cggctcattg gccaatgtct aggactgcca
     4561 catcctctcc ctgcaaccga atcgttacct tacggttcac aacggtcaac gttttggctg
     4621 tgacaagttc ttgaactgtc gccggcaggg tgagatcggc gtcaaaagtg acctcggctg
     4681 ttgcctcctg ggatgcctcg ttgaaaacgg tgaaataccg atcgccgaat ctttccacat
     4741 agacattggg gacattgcat tgcgcccgtg taatgggttc ccagccagcc tcggagagcc
     4801 gctggcaaag gggcacatat ttcttgaaaa ggggtcgatc gcgattgtac agttcgggcc
     4861 gggtgaaata atgtccccct gaagcattgg gactgaagaa accagggaac atgccatagg
     4921 cgacacatcg tttcatatat ttttcaacaa gcgggtaatc aaatcggtcg aaattggtgt
     4981 tcatcaggaa acagtaaggt tttttgccgc acagggcccg tcggaaaaga agctccgagt
     5041 cactcatggg ccgccactga ccgttggggt tccagtctgt ttcagtgccc atcacgtcca
     5101 gcaatggtac aagccaacat agatgtgatg gtgtcccgtt ggccatcata aacttgccca
     5161 tcgcgtggac atcctcggcg atggcgcgga catactcaaa aacaataagg ccgcggaaga
     5221 ccgccggctg aaaacttgaa gcgtcatatg tcaacggacg ctcgccaaca aagtggtccc
     5281 ggcggaagtc gagctcatcg gtgacatagc cctcgcttga gtcgatgtac tctccgtcca
     5341 aatctccttt gcgatttggt ccgtataggg cttggcgaat atcaggattc catttgagct
     5401 taaaatcggt aatctcgccc cgaatctggg ggagggaatt catgctccac acggccccgt
     5461 tacaccatgg ggtgtctagg agcctcaacg ggattcggcc attttcatcc cgaaaactac
     5521 tcgacagcca cgccttcgct tgaggtcggc cctgttgagc caaccgttgc gcctcggcca
     5581 cggcggcctc gtacgttctc ggtatctcag gggccatggg catccaccaa gtcatgggct
     5641 ccgtgtagcg gaacgtcaaa atgtcatgag cgtcatccca ctgtgtttcg ttgttgcctt
     5701 ctttgaagcg gaaaccaaag tcctcccagt gttcaacctg cgagatcttg gcaaatggca
     5761 tccattgccc atgttttgaa attcgagagg aaaaataatc aggaaagatc tctatatagc
     5821 gagcccaagc cgcccgaaag cctccatgcg gagaaaaagt gaaaaccacg aagcgcaaat
     5881 gcgcatctgg cttctctttg gttagagcga tatcgtacgc aacgaatagt tgttcgtaag
     5941 cggcgttata gcccacgcgg aagaccgctg gataaatcat gtcaatccca acaccgtacc
     6001 cctgggtgtc agttgccacg gctgctaggg gccatcgtcc cagccgctga gtagcccccg
     6061 cgcgtgttcg gacggtcgtc gcgtactcgc gggttgccga aacagcctcg ctgcggcggg
     6121 ggctttgaag ccaaagaatt ggtttgtcac cggacgacgt gcatttgacc ggctcgacaa
     6181 aggcaaccgt tagggctcga tcttcgtcag atcgactccg caggataagg tcataggttc
     6241 tgacgttatc tttttcggtg acggtccaga cgcactcaat ccccaaggcc gactgttgaa
     6301 gggccacaaa gtctgagttt ttcgccacat cacgaacgac caacccccgg taatccccaa
     6361 cggccttggt gcagggaatt ccgtcaaaga gggcagttcc cggcgtgctt atttcgtaca
     6421 aacgcggatt tcgaaacatc gctttacccc gatgcccgcg gaaaagcaaa taataggaca
     6481 atcgtcggat gggcttttcc ggaaagatga cgacccttcg ctcttgccag tcgtgggtgc
     6541 ccacatcaaa cgccgccgcc tggccccaaa ggggtgttcc gtcgttgtac tcgatatcaa
     6601 gatatacgga gtaattgtta tctcgggcac cgtccacgtc ctgggccgca ctttcggcca
     6661 cggcgaccag aggccgtggt tctttttggt tcaagacaat cgactgaacc gccccggcac
     6721 gatatcccgg cttatccttg ttttcgcaga cgataacacc ctcgtgctct tcaaatcctt
     6781 gttcgtattt tccccaagtt ccttcggcga ggagattctt cccttcctgc tggccccgaa
     6841 tcacggcgaa aatgacttgc ccatcgccag cactttcgcc cacggcgacg gtggttattc
     6901 caaaggtcac taaaactatc agcataccct gccaaattcc gaacaaatga catatttgcc
     6961 gtcggttatc aggatattgt ctggtccaca tttctgtgtc ctttcgtcca ttggcatctc
     7021 gaagatacaa aggtcgcatt cctaccaagc aatccgaata tctaagcttt gtgcctggtg
     7081 gacaacgacg ggttcctcaa aaacgcacga aaatcggcct tcgtcacacc gcagcacaca
     7141 cgggatttcg cgtttttcta ggacacactg aactgttttc ggtgtggttc cggcaggtgt
     7201 cgccccctca aatgtcttga cggcgagttg gccgaagcga acactcaggc tatggttctg
     7261 ctcaccatca ccccgccatt gacgataaac tccccaaccc tctgcggctg tgaaaagtga
     7321 tacgtggtcc tccggctgcc agaccggaat aaacccaatc cgttgatctg gcccatcgta
     7381 aataaaacct tgacatgcca ggaggattga ccacacgctc atcgcgcggg catagaactt
     7441 gccacactcg tcatcaccaa agggattgcc cgaatacccc caattgttaa aattgcctgc
     7501 accagggaag acccgtttcc gaccatcgta acgaagggca atcgctcgac agaccgttag
     7561 cccttctttg agtaatcccg cctggatcat ggcggcagcc gccgcgtact caaaacctgt
     7621 catgacctca ctggcatact taatgatttg actcggtttt ttggccccct ctggccagac
     7681 gaacatctgc atccccccgt cctcgtcagc cacgaatttt cgcggcaatt ggggcaaacc
     7741 ttccatgcgg cctcgaaaat tgagcgcgaa gagagtcctt agcgccgacg tcacatgctc
     7801 tggcggataa acccagccca agtcaagctg atgagcccac cattgtccaa gcacctggtc
     7861 gatggcacat ccttccccgt agtcctcgtg gggtgttgga tcgggaattt gaatgtagta
     7921 gcgcccgttg aacagtagtt catcttgttt ggccgcgcct aacctggcta tccgctggta
     7981 cttctcagca gaggtgatgt caccctcaag ccgggccatc ttttcggcag cggtaagagc
     8041 cgccagatag agacttccca gccaggatga attgccgcct aactcgccgt caagtgtgtt
     8101 ccattgtggc cctgacaaca gcccgtcctg atccgggtcc caacgttgaa ccacgtagtc
     8161 catggcccta cgaatcgcag gccagtgtga gtctagccaa ctcctgtccg acgacatcag
     8221 atgctcccga tacgagttaa gcacggcacc gcattgccca tcaaacgcag ggaaagaatc
     8281 tggttgccga tgggggatcg ccccgtccgg cttctgataa gtaaactcct gctcccgcat
     8341 ttgttttgcg atttcgggga acaatcgggc atgggcctgg gcgtaatgcc agacgtgatt
     8401 gcaattaccg tgacagcatc cttcgttaac gttgcaacct tcccacccac cgaaataacc
     8461 ttctcggcac caaaagacag tcggtgaccg caagatggcc acttgggagg caatccggtc
     8521 gataagccaa tagggtagat ttgattcata aagagagtcc acatacattc tcgatgctgc
     8581 ctcaagttca ggcaggcgct gttctaactc ggctgctacc tcctgggcat tcttccacca
     8641 agcctcatag cgccgccctt tgcaccccca ggccccggtg ccggcaggtt ggccgggtaa
     8701 ataccacact aagacatacg tcacttcacg cgtctccccc gggggaatat ccacgcaact
     8761 aaacaccgca ccattaaccg tctccccttc ccgcgaggtc gcagttgtta cctcctggag
     8821 tgactcaatt tgagaagggg cacgcaagag tccggtgagc gattcagagt cctcccaatt
     8881 ggccagaacc tttgcacggg gactgtcaac catgaggacc atctcgccgg catccggcgc
     8941 gtcctgcttt gtcatgcaca gccaggtgcc cttatcggtc cgcagaacac tgttgacatt
     9001 tttcccaaat cgttcgtacc gccgctcatc aatcggttta tctgcttgat aaccaacaac
     9061 gttgagctgt gtccccagga tcgcggccgt gaccggttcg ttcatcggat tgtggatacc
     9121 aatccgatac acggcgcagg gaatggctga atcccgggca tgcaacggaa tgagcgggtt
     9181 gaaagctgtc agggttacat ggcacgatac ctcttcgtcc tggaaacgat aaacagcaaa
     9241 cggatagggg gcttccaaac gcagccgttg catcggcgga atcccggcca cggacgtggt
     9301 ctgaagagtc cgcacaatcg atcgcgtggg tgttgataca ccgatggcga agaagctctc
     9361 cgggatttgg cgataggcca cgctaccaaa aatctgccaa gcgtgacgtt cggcgcggcc
     9421 attcatgtga atagcaccca cgccaatacc accgatagga atgtcaatgc attccagacg
     9481 ttccgcctca taaacggtcg gctgtccccg cgtgacaagt gctggatcgt tcatcaattc
     9541 catccgccgc ttttcacgca agtccgcaag tcgggcctgc tcctctgcct gtcttttgag
     9601 gatttcctga tattgttgcg cgagggccaa ccgctcatcc cggaagccga gctcatcggt
     9661 tcgtgctgcc acttgttccg cggtccacgc agtatcataa atgacaacat tggccaaagc
     9721 ccctaagaag gcctcatgtc cggaaggctg agacgcagca agcgtgagcg gacgtccctt
     9781 ttcctggata ttgatcgtcc aagaaggcga gtccgtgatg cacgggaccc catcgagata
     9841 aacggttgtg ccggattttt gcgtcacgat gaccagatga taccacactt cgggtttgag
     9901 tgcccccgca ggtgatcgaa acacggcaac gctcttgcca ttccacaagg caattgccga
     9961 atagtcgccc atgatgtgaa cactccaccg cgtttgccgg tgatcaccgg attctcgttg
    10021 agcaatcaag cacggattgt acctcgccgg tgccgagaac tggcccttaa aacaaatctc
    10081 aatggtcagc tgcggtacgt ccaggtgaga cgcctcttga aaaacgatcc aggtgttccc
    10141 gtcaaaacgg aggcctttca aattctcaga atcggagaca acttgtggcg gtcccccaac
    10201 gattcgcccg cccgtctgtc caagggcctc caaatgcgcc ggatcaagat aaaacaaccg
    10261 gcgaccctga gcgtccgaaa cttgcacctc ggctgttccg acccgtgtgg ctaatggttc
    10321 cgtgaaagcc caagctcctg gtaaacagac cgtaagcagg aaaagc
//
LOCUS       DAOSUT010000145        10365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3592_length_10365_cov_9.472939, whole genome shotgun sequence.
ACCESSION   DAOSUT010000145 DAOSUT010000000
VERSION     DAOSUT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_10365_cov_9.472939"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLD05_12260"
     CDS             complement(<1..191)
                     /locus_tag="PLD05_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOP78260.1"
                     /translation="MGNTQTLASSQPKNVVIRASAGAGKTFQLTNRFLWLLARGERLD
                     SILATTFSREAAGEILDRIL"
     gene            complement(244..2952)
                     /locus_tag="PLD05_12265"
     CDS             complement(244..2952)
                     /locus_tag="PLD05_12265"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP78261.1"
                     /translation="MWRSQQLVLNFFLAVVLVVIGHFNSGSLVWGQNIVNGGFEQDAD
                     NDGLPDEWVTAGRSSIRQTLTRVLEPGRGYVACLKCTHFEGGFPDSHVMLAQLNRVGV
                     TRGQWYRLRLWARAEDLEFGAVSISLVNRKIWQDVGLRETFSPRADWKPYEFHFRATR
                     DLAPEDSRFQIYFQGTGTLFLDDIVLEPVSGFRPQRLPQLPTKGLKNLLVDSSFECGA
                     SDWGTVALGITSWAGNLFHRVGTWDSTKAYHGTHSWRISLSQSHPLISYFDYFDPVAT
                     VVRSVVIANKGWIPLEKGNRYVLSAYVCADQTDVPVVLLVRQETRTIQQSFRVGPQWT
                     RFSLSFVAEYDFAYCGVGIDLQHSPLAEATIWVDAVQFERVDDVNAGPSDYRPARSVE
                     SAISTDRMGNIFLTPERGFDITLRAFNGSTDPQCLEGNLRILDYKDRPIWQKDVRLDI
                     PQQSMAEERFSQLLAGRRGFFRVTWEPQGAPPQSLRIANIDPVIEQDTAFGMNHALPW
                     DFLLELSHAAGLRWWRDWSCQWRLVQKTEGGPFDFQIPDTQIKRVLQAGGNPLVLLPF
                     PATEWSAIVDTDRVAREAGSNSYLQRRLIVAQKPRDVVEFGQYVRATVEHYHKSVKVI
                     EILNEPLFTSYALPNSFGWNISDYLDILKTAYESAKAVDPECLVIGGIAAPPESNWVR
                     QFIEQGGLQWCDVMNLHLYPHRGSPDAYESSFAACHRLMEARGQVRPIWVTEIGCYAD
                     DDPPTLPFSVGDEAMNRSLRPSEYRAAIDLVKFAAVMGAAGVKKVFYHAGTCGALNEN
                     TAGNIFFEYGGEPRKMYAAQAVLSDFLGADWNFVGKWGQDQMLQGFRFQSKGREVIVL
                     WSRVPTTVSVPQGYQVWDIMGNPSDLSLQEVTDEPVYLIRHIPNQN"
     gene            complement(3124..4050)
                     /locus_tag="PLD05_12270"
     CDS             complement(3124..4050)
                     /locus_tag="PLD05_12270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOP78262.1"
                     /translation="MRTRWVIAGTLLLIAGCVGVFIFLVSTPGLRLMSSKNPPRSTEA
                     FAVEKSAFGGNGSSSVSKDDPVNPAEQSEEKIVSGPCRPVFLEEPEAFRLARQRMVQF
                     QLQNRDIIDADVLYVMGRIPRHEFVPENVIHEAYDDHPLPIGHGQTISQPYIVALMTQ
                     LAKPRKESRALDIGTGSGYQAAVLAELCREVYSIEIIEPLAKSAAERLKRLGYTNVVV
                     RAGDGYRGWPEKAPFDVIIVAAAPDHVPKPLVDQLALGGRLVIPVGRGQQDLLVIEKQ
                     PDGSIRQTSVAPVLFVPMTGEAQQLGKSSSNP"
     gene            complement(4169..>5065)
                     /locus_tag="PLD05_12275"
     CDS             complement(4169..>5065)
                     /locus_tag="PLD05_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78263.1"
                     /translation="IPPEVDFNLWSGPAPFTTPRCTRPNFHYDWHWQRLYGNGDLGNQ
                     GPHQTDIARWGLGINSHPISVISYGGRLGYQAERNDDNYVDAGDTANTEVSIYDYGDK
                     CIVFETRGLSVDNSADEEINVLFESKTGNKIGVIFYGTDGYLVQQTYTRCVVFDKNLK
                     PVKTFGGSGNHFANFIDACLSRDPKMLNSDAWEGHLSAALSHLGNISYYLGENNKVSP
                     EEAKRILAGVKSLDDNIATLERTLRHLQDNGVDLKKYPISMGPLLKFDPEREVFPDSP
                     EATALVAREYREPFVCPKADKV"
     assembly_gap    5066..5165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5166..5533)
                     /locus_tag="PLD05_12280"
     CDS             complement(<5166..5533)
                     /locus_tag="PLD05_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78264.1"
                     /translation="MKQTWTRRQFVGTSLGALASAIAVPQFLRAEGANEKVGIAIIGV
                     RGRGGEHIDAFLSDPRVQILYIVDADEKVGQGRVEQIYKAKGFRPKFIRDFRQALDDA
                     AVDAISTATPNHWHALCGVWA"
     gene            complement(5731..6816)
                     /gene="holA"
                     /locus_tag="PLD05_12285"
     CDS             complement(5731..6816)
                     /gene="holA"
                     /locus_tag="PLD05_12285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOP78265.1"
                     /translation="MTPSPQKATSLRPPSGLELILGELSSPPPFCVVYGDDDYLRTLV
                     LHKLRRHWLPDAEDEFCLREFDGNEVNWTAVEKELTTVSMFSSRPRVIIIEGADRFVA
                     EHRGELEKLLSGSPLRNVLMLSVSQWSANTRLAKLALERGLVVECDFARKKGQELIPW
                     MIRWAKHQYSLSLNRDAAEILLESVGPICGLVDQELKKLAGLGKKTVTAEMVASLTGT
                     WRTKAVWDVINAALAGKPGESLGEIQKLLEAGETPLSILGMVAPMLRRLNMATQRYLF
                     PPPGQRRSSLQESLEEAGVPAFALKAEEERLRKLGRQRALKLLHWLRSADAAMKGAIP
                     TDPRLVLERLIVVLGATACSGGDCDPV"
     gene            complement(6824..7483)
                     /gene="eda"
                     /locus_tag="PLD05_12290"
     CDS             complement(6824..7483)
                     /gene="eda"
                     /locus_tag="PLD05_12290"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOP78266.1"
                     /translation="MMDNPHLSSILSCGICAVIRANSGAVLGDVAEALAEGGVTAIEV
                     TFTVPRAHRVLEAVADRLGDRIVLGAGTVLDPETARIAILSGAEFIVSPTLNVNVIEM
                     CKRYSKLVFPGAFTPTEILTAWQAGADIVKVFPSDVTGPGYLKAIHGPLPYIRLMPTG
                     GVTLETAADFIRAGACVLGVGSALVSSRAIAAKDFDQIRELARQYVNIVREARQGNTT
                     K"
     gene            7779..8018
                     /locus_tag="PLD05_12295"
     CDS             7779..8018
                     /locus_tag="PLD05_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78267.1"
                     /translation="MLRLLHSLILIVPGLFVVIVLVVLGTWWLQRLRRALYQDSSDSS
                     DELAQQFFKMRRDGQISEEEYKVIRANLSSQRRTS"
     gene            8297..9601
                     /gene="clpX"
                     /locus_tag="PLD05_12300"
     CDS             8297..9601
                     /gene="clpX"
                     /locus_tag="PLD05_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOP78268.1"
                     /translation="MPTGRDFTSGRRGPTSGKKNAFCSFCRKSYRDVGPLVEGPGDVY
                     ICGECIDLCQSILDQERKRKGTARPLFARVPTPREIVAKLDEFVVGQEEAKKVLAVAV
                     YTHYRRLMHHGTSDVVLDKSNILLIGPTGSGKTLLAQTLARLLDVPFAIGDATTLTEA
                     GYVGEDVENLLLKLLHAADFDIEAAQRGIVYIDEIDKIRKTSHNVSITRDVSGEGVQQ
                     ALLKMLEGTISNVPPQGGRKHPEQQYIQVDTTNILFICGGTFVGLEDIIARRMGKRSI
                     GFASSVPSSRGDREGGEASRDEYLLAHVTSEDLVEFGLIPEFIGRLPVVTALRPLDVD
                     AMVRVLVEPKNALVKQYEELFAMENAELEFTEEALRLIAQKAYEKGTGARGLRAIVEG
                     IMLDALYQLPDQPPGFRYVVDVDVVEGRKPLLGRAIARQKTA"
     gene            complement(9802..>10365)
                     /locus_tag="PLD05_12305"
     CDS             complement(9802..>10365)
                     /locus_tag="PLD05_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOP78269.1"
                     /translation="LADKHGIALVSDEIYRDFCYDGPFTSPAQFYPKTIVVEGLSKSH
                     GMPGWRVGFAHGPKAIIQQMIKLQQYSFVCAPQPFQWAAATALDCDMSAQYSAYRRKR
                     DMVVQGLQGYYELQIPEGAFYAFPKAPWGSATEFVTTAIEKFQLLIIPGNVFSRRDTH
                     FRISYAASDETIQKGIEVLREMAHRPA"
BASE COUNT         2306 a         2811 c         2848 g         2300 t
ORIGIN      
        1 aggatgcggt ctaggatttc tccggcagcc tcgcggctaa aagttgtggc gaggatgctg
       61 tccagccttt cgcctcgtgc cagcagccaa agaaatcgat tggtcaactg gaaagtcttt
      121 ccggcgcctg cactggcacg gatgaccaca ttcttgggtt gtgagcttgc cagtgtttga
      181 gtgttgccca tcgtgttagc cccttagcgg atcttgttgg aagcgttttg gtaatgcatt
      241 gcgttagttc tggttgggaa tatggcggat gaggtaaacg ggttcatcgg tcacttcttg
      301 cagcgataaa tcgctgggat ttcccatgat atcccaaacc tgatatcctt ggggaacaga
      361 tacagttgtc ggaactcgag accagagcac aatgacctcc cgcccttttg actggaaacg
      421 gaacccttga agcatttgat cttggcccca cttgccgaca aagttccagt cggctcccaa
      481 aaaatcggag agaaccgcct gggccgcgta catctttcgg ggttcacccc cgtactcaaa
      541 gaaaatgttg cccgcggtat tctcattgag ggcgccacag gttcccgcat ggtagaagac
      601 tttcttgacc ccggccgcac ccataaccgc cgcgaacttg acaaggtcga tggcggctcg
      661 gtattcgctc ggtcgtaagc tgcggttcat ggcctcgtcc cccacgctga acggcagggt
      721 agggggatcg tcatcggcgt aacagccgat ttccgtgacc caaatgggcc gcacctgtcc
      781 gcgggcttcc atgagtcggt gacacgcagc gaaggaggac tcgtaggcat cgggacttcc
      841 tcggtgggga tacagatgga gattcatcac gtcacaccac tgtaacccgc cttgttcgat
      901 gaactgtctt acccaattcg attccggcgg tgcagcgatt cccccgatga caaggcactc
      961 gggatctact gcttttgctg actcgtaggc cgtcttgagt atgtcgagat agtcagaaat
     1021 gttccaacca aaggagttag gcagggcata gctcgtgaaa agcggttcat tgaggatttc
     1081 aatgaccttg acgctcttat ggtagtgctc aaccgttgcc cggacgtact ggccgaactc
     1141 caccacgtcc cttggttttt gtgccacaat aagacgccgt tggaggtaac tattggagcc
     1201 ggcctcccgc gcgacacggt cggtgtccac gattgccgac cactccgtag ccggaaaggg
     1261 taggagcacc agaggattgc cgcccgcttg gaggaccctc ttgatttggg tatcggggat
     1321 ctggaaatcg aaggggccac cttcggtttt ctgcaccaag cgccactgac aagaccaatc
     1381 ccgccaccag cgcaacccgg cggcatggga gagctctagc agaaagtccc aagggagagc
     1441 gtgattcata ccaaatgccg tatcttgttc gatgacgggg tcgatattgg ctatccgtag
     1501 cgattggggc ggggcccctt ggggttccca ggtaacgcgg aagaatcctc ggcgacctgc
     1561 gagaagttgg gaaaaacgtt cttcggccat gctttgctgc gggatgtcca atcgtacatc
     1621 cttttgccaa atgggccggt ctttgtaatc gaggatgcga aggttgccct ccaggcattg
     1681 cggatccgtt gatccattaa acgctcgcaa ggttatgtcg aagccacgct ctggcgtgag
     1741 gaaaatgtta cccatccgat cggtggaaat cgctgactct acgctgcgag caggtcgata
     1801 atccgacggc ccggcattga cgtcatccac gcgttcaaat tggacggcgt caacccaaat
     1861 ggttgcctcc gccaaaggag agtgttgtag gtcaatgccg acgccgcagt acgcaaagtc
     1921 atattcggct acaaatgaca gcgaaaaccg cgtccattgt gggcccacgc gaaaactctg
     1981 ctggatcgtt cgcgtttcct gccgcacgag gaggacaacg ggcacgtcag tttggtcagc
     2041 gcagacatag gctgaaagta cgtagcgatt gcctttttcc aggggaatcc aacccttgtt
     2101 agcgatcact acacttcgta cgactgtggc tacgggatcg aagtagtcaa agtacgagat
     2161 cagcggatga gattgggaca aggatatccg ccaactatga gtgccatggt aagcctttgt
     2221 cgagtcccac gtccccacgc ggtggaatag gtttcctgcc caggatgtta tccccagggc
     2281 taccgtcccc cagtctgagg cgccacactc gaagctgctg tcaaccaaaa gattctttag
     2341 ccctttcgtc ggtaattgag ggagtctttg gggacgaaac ccggagactg gctcgaggac
     2401 gatatcatcc agaaatagag ttcctgttcc ctgaaaatag atttgaaaac gactatcttc
     2461 cggggcgaga tcacgagtag cccggaaatg gaattcgtac ggtttccagt cggctcgggg
     2521 cgaaaacgtc tcgcgaaggc ctacgtcttg ccaaatcttc cgattaacaa gtgaaatcga
     2581 aacggccccg aactcgaggt cctcagcgcg ggcccaaagt cgcagacggt accactggcc
     2641 gcgcgtgaca ccaacccgat taagctgtgc caacatcaca tggctatcag gaaaaccgcc
     2701 ctcaaaatgc gtacacttca aacaggcaac gtagcccctt cctggctcga gaacccgcgt
     2761 caacgtttgc cggattgaac tccgaccggc ggttacccat tcatccggca gaccatcatt
     2821 atccgcatct tgttcaaatc cgccattgac aatgttttgc ccccaaacca gtgagccaga
     2881 gttgaaatgc ccaataacga ctaagacaac agcgaggaaa aagttaagta cgagctgctg
     2941 agacctccac atgggacata cctctatgat tactggcggg ctcgagtggt ttggtacctg
     3001 ccccctattg tacagagggg cctatacaga gggagagtgt cggtgatggc tgcggcgcac
     3061 tttgctcgca gagactaacg agatgagagc cgcttaatct tcgccagaca aaatgaggac
     3121 accttacgga ttgcttgaac ttttgcccag ctgctgtgcc tcgcccgtca tcggtacaaa
     3181 taatacgggt gcaacactgg tttgacgaat ggaaccgtca ggttgcttct caattaccag
     3241 cagatcttgc tgaccgcggc caacaggtat gaccaatcgt ccgcccaagg cgagttgatc
     3301 caccaagggt ttggggacat gatccggggc ggctgctacg atgattacgt caaaaggagc
     3361 cttttccggc cagccgcgat agccgtcgcc cgcccggaca acgacattgg tgtaacccaa
     3421 ccgcttgagt cgttccgcag cggatttggc caaaggttcg atgatctcga tgctgtacac
     3481 ctcgcggcac aattcggcga gaaccgcggc ttggtatccc gatcccgtac cgatatctaa
     3541 ggcacggctc tctttccgcg gtttggccaa ctgcgtcata agggcgacga tgtacggctg
     3601 tgatatcgtc tggccatgcc cgatcggcaa aggatggtcg tcatacgcct cgtggatcac
     3661 gttttccgga acgaattcgt gacgtggaat ccgtcccatc acgtacaaaa catcggcgtc
     3721 gatgatgtcg cggttctgaa gttggaattg aaccatccgc tgtcgggcca agcgaaaggc
     3781 ctccggttcc tctagaaata ccggccgaca cggaccgctt acgatctttt cctcactctg
     3841 ttcggcaggg ttcacgggat catccttcga aaccgaactg ctaccgtttc ccccgaacgc
     3901 cgacttttcc acggcaaatg cctcggtcga tcgaggggga tttttggaag acatcaacct
     3961 caagccgggg gtgctcacca aaaaaatgaa aacacctaca cagcccgcaa tgaggagcaa
     4021 cgttccggca ataacccacc gcgtccgcat agctcacctc ccgattgttt ggcccaagct
     4081 tgcggaaacc ggtccaaatc ctctcgcgat ggcgacaata tcacagcctt tatcgcctat
     4141 tgtcacccca cgaacaaagt ggattgtgtt acaccttgtc ggctttgggg cagacaaagg
     4201 gctctcgata ctcccgagcg accaaggccg tagcctctgg cgaatctggg aagacttccc
     4261 gctcgggatc gaacttgagc aacggtccca tcgagatcgg gtacttcttc agatcgacgc
     4321 cgttatcctg gagatgacgg agcgtccgtt ctaaggtcgc gatgttgtca tcgagacttt
     4381 taacaccggc caggattcgt ttggcctctt cgggtgagac tttgttgttc tctcccaaat
     4441 agtaagagat gttacccaag tgactgaggg ccgcggagag gtgtccttcc caggcatccg
     4501 aattgagcat cttcggatcc cgactgaggc acgcatcaat gaagttggcg aagtgattgc
     4561 cggagccacc aaatgtcttg accggcttta ggttcttgtc gaataccacg caccgcgtgt
     4621 acgtttgctg gacaagatag ccgtccgtgc catagaaaat gacgccaatc ttgttgccag
     4681 tcttgctctc gaagaggacg ttgatctctt catcggccga attgtccacg gacaacccac
     4741 gcgtttcaaa gacgatgcac ttatcgccgt aatcgtagat agacacctcc gtatttgcgg
     4801 tatcaccggc atcgacgtag ttgtcatcgt ttcgttcggc ttgataaccc aaccgaccac
     4861 cgtaggagat cacactaatg ggatgggaat tgattcccag accccagcgc gcgatatccg
     4921 tttgatgcgg cccctgattg cccaaatcac cattcccgta taagcgctgc cagtgccaat
     4981 catagtggaa gttgggccgc gtgcatcgcg gcgtggtgaa aggtgccggg ccgctccaca
     5041 aattgaaatc gacctcgggc ggaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnngccca aacaccacac aaggcgtgcc aatggttagg agttgccgta ctgatggcat
     5221 ccacggcggc atcgtccagt gcctgtcgga aatcccgaat gaacttgggc cggaaaccct
     5281 tagccttata aatctgctca acccgccctt ggccgacttt ttcatcggca tccacaatat
     5341 agaggatttg gacgcggggg tcactgagga aggcgtcaat atgctctccc ccgcgaccac
     5401 gcacaccaat aattgcgatg ccaactttct cgttggcacc ttcggctcgc aagaactgcg
     5461 gcacggcaat agccgacgct aaggctccca gcgaggttcc cacaaactga cgacgggtcc
     5521 aggtttgttt cattggacac tccccaatct ctcagataga tcactctcga aaacccaacc
     5581 gcgaactcgg cggcaacttc ccgtttgtga atctcacagg acgtcaaacg gtggccatcc
     5641 cgaaccgttc ctcacacttt gcacaattta ccagcgccgg tgtcaattgt ccagaaactc
     5701 gggacgatta atctcagagg gacgaacgca tcaaaccggg tcacaatctc cgccactgca
     5761 tgcagtcgct ccgaggacaa caataagccg ctccaaaacc aaacggggat cggttgggat
     5821 ggcacctttc atggcggcgt ctgccgaacg aagccaatgg aggagtttga gggcccgctg
     5881 tcgccccaac ttgcgaagtc gctcttcctc ggccttgagg gcgaatgccg gtacaccggc
     5941 ctcttctaga gactcttgaa gcgatgaccg tctctggccc ggtggtggaa aaagatagcg
     6001 ctgggttgcc atgttgagcc tgcgcagcat tggcgcaacc atccccagaa tactcagggg
     6061 cgtttctccg gcttctaaca gcttctggat ttcgcctaat gactcgcccg gcttgcccgc
     6121 cagggccgca ttgatgacgt cccacacagc cttagtgcgc catgtgcctg tgagcgaagc
     6181 caccatctcc gccgtgaccg tcttcttgcc caaaccggcg agtttcttga gttcctggtc
     6241 taccaacccg catatcgggc cgaccgattc caagagaatc tcggccgcgt cacgatttag
     6301 gctcagggag tattggtgtt tagcccagcg aatcatccaa ggaatcaatt cctggccttt
     6361 ctttcgggcg aaatcgcatt ccacgactaa tccacgttca agtgccagtt tggctaaacg
     6421 ggtattggcg gaccattggc tgacggataa catcagcaca tttcgaagtg gggaaccgga
     6481 caatagtttt tccaactctc ctcgatgttc cgcgacgaag cggtcggcac cttcaatgat
     6541 gatgacccta ggccgtgagc tgaacatgct cacggttgtc aactctttct cgaccgctgt
     6601 ccaattgacc tcatttccgt cgaattcgcg gagacaaaat tcatcctccg catcgggcag
     6661 ccaatgacgc cgaagtttat gcaagacgag tgtacgaagg taatcgtcat ccccataaac
     6721 gacacaaaat ggtggcggag atgagagttc gccaaggatt aattccagcc ccgacggcgg
     6781 tcgcaaactc gttgccttct gcggactggg tgtcatgacg gcctcatttt gtggtgttgc
     6841 cctgtcgcgc ttcgcgcaca atgttgacgt attggcgggc cagctcacgg atctgatcga
     6901 agtccttagc ggcaatcgcc cgggaactca ccaaggcact accaaccccc agaacacaag
     6961 cccccgcccg aatgaagtca gcagcggttt ctagtgtgac accccctgtc ggcataagtc
     7021 gaatataagg cagggggccg tgtattgcct tcaaatagcc tggtcctgtg acgtcggacg
     7081 ggaacacctt gacgatatcg gccccagcct gccaagcggt taggatttcg gttggtgtga
     7141 acgccccggg aaaaaccaat ttgctgtagc gtttgcacat ttcgattacg ttgacattca
     7201 gtgtggggct gacgataaac tcggctcccg aaagaatagc aatacgcgct gtctctgggt
     7261 ctaggacggt tcccgcccca aggacgattc ggtcgcccag tcgatcggcg actgcttcga
     7321 gcacgcgatg agcacgtggg acggtgaacg tcacttcgat tgccgtgacg ccgccctcgg
     7381 cgagggcctc ggcgacgtcc cccagcacgg caccactatt ggcacggatg acggcgcaga
     7441 ttccgcaaga aagaatactc gaaaggtgtg ggttatccat catataaagc tcatcagcaa
     7501 cttggttaaa ctgaggtact tgcgacgctc ctcctgagct tattgcgatt cgcggggggt
     7561 gtcaactcgg tcgccggttc gaacatgcgg aagggtcata accgccgaaa gtcctcctgt
     7621 cgctggacga tcgtcctaaa ccgcgcgata atgatgacgg accaccgagg ataaggtgcc
     7681 cggtcagtgc gtctcggcgg cggtttggtc attgtgcgaa tgtcccgttg agcgaagtct
     7741 gacacccatt tggaccgtgt gaggagagac ttggcgggat gttacgtctg ctgcactcgt
     7801 taatccttat tgtaccagga ctttttgttg ttattgttct cgttgtgctc ggaacgtggt
     7861 ggcttcagcg tctgagacgt gccttatacc aggactcaag tgacagtagt gacgaactgg
     7921 cgcagcagtt ctttaagatg cgcagggatg gtcagattag cgaagaggag tacaaggtaa
     7981 tccgggcgaa cttgtcgtca caacgacgca cgtcgtgatg aagcgaaaac tgggcttcgg
     8041 ggctatgtgg tagaatttag gtaaaagacg aaggttgcgt gaaactcttc gggcgatgcg
     8101 tcgcgtaatg attagcgaaa gggaggacac acgccgcgtt gaacggcggt ttttgtcccg
     8161 atatcgccga tgaccaatca atgtcagggg aatgactgag gattgccagc ggcgatcatc
     8221 tccgtagggg agggcagggg ggctggcggc ttggacttcg acgtttcaac tttttgtgac
     8281 acccagggtt tgaagcatgc cgacaggacg ggacttcaca tcaggacgcc gtggtccaac
     8341 gagtggcaag aaaaacgcct tttgttcgtt ttgccggaaa agctaccgtg atgtaggacc
     8401 gttggtcgaa ggaccagggg atgtttacat ctgcggggag tgtatagacc tttgtcaatc
     8461 cattcttgat caggaaagaa agcgcaaggg aacagcccgc ccgctttttg cgcgagtgcc
     8521 aacccctcgc gagatcgtcg ctaaacttga cgagtttgtt gttggccaag aggaggccaa
     8581 gaaggtcctg gccgtcgccg tgtacacgca ttaccggcgg cttatgcacc acgggacttc
     8641 ggatgtcgta ttggacaagt caaatatcct cctgattggt cccacggggt cgggaaagac
     8701 gcttttggcg cagacgcttg cccggctgct ggatgttccc tttgctattg gcgatgcgac
     8761 aaccctaaca gaggcgggtt acgttggaga agacgttgaa aacttgctcc tcaagttgct
     8821 gcatgcggcg gactttgaca ttgaggcggc acagcgcgga atcgtctata tcgacgaaat
     8881 tgacaagatt cgcaagacga gtcacaacgt ctccattacc cgggacgtct ctggtgaggg
     8941 ggttcagcag gccttgctta agatgctcga agggactatt tccaatgtcc caccgcaagg
     9001 cggacgaaag caccccgaac agcaatacat ccaggttgat acgacgaaca tcctttttat
     9061 ctgtgggggc actttcgtcg ggctggaaga catcatcgcc aggcggatgg gaaaacggtc
     9121 gattggattt gcctcgtcgg taccgtcatc ccgtggggat cgcgaaggtg gcgaagcgag
     9181 tcgggatgag tacctcttgg cccacgtgac gagcgaagac cttgtcgagt ttggcctgat
     9241 tcccgagttt attggccggt tgcccgtggt cacggcactc cgcccccttg atgtcgatgc
     9301 catggtgcgt gtcttggtcg agccaaagaa tgctttggtc aagcagtacg aagagctttt
     9361 cgccatggaa aacgcggagt tggaattcac cgaggaggcg ttgcgcttaa ttgcccaaaa
     9421 ggcctacgag aagggcaccg gtgcccgagg tctgcgggct attgtggagg gtattatgct
     9481 cgatgccctc tatcaattgc ccgaccaacc gccgggcttc cgctatgtgg tcgatgtgga
     9541 tgtcgttgaa gggagaaaac ccctgttggg tcgggccatt gcccggcaaa aaacggcctg
     9601 agaggccttt tgacgtgatc acaagtcgtt tcgtttctaa ggttctgctt aaagcctctc
     9661 ccctcgcccg ttcggcgggc gggggtaagg gggttcgaat cgctgccgct ttttctcgat
     9721 cgattccgac gttcgcttgc cctcacccca accctctccc ggaaggagag ggggactttt
     9781 tggcataggc tctcatattg atcacgctgg gcggtgggcc atttcgcgaa gaacttcaat
     9841 ccctttttgg atggtctcat cggaggcggc gtaagatatc cggaagtgcg tgtcgcgccg
     9901 actgaataca tttccgggaa taatgagcaa ctgaaacttc tcaattgcag tggtaacgaa
     9961 ctcggtagct gatccccagg gggccttggg gaatgcgtaa aatgcccctt cagggatttg
    10021 tagttcgtag tagccctgta acccttggac taccatgtcc cgctttcgcc gataggcact
    10081 gtattgtgcg ctcatatcgc aatccaaagc cgtagcggcc gcccactgga agggctgtgg
    10141 ggcacataca aagctgtact gctggagctt gatcatctgt tggatgatcg ccttcggacc
    10201 atgggcaaag ccgacccgcc agccgggcat gccgtgactc ttgctgaggc cctcgacaac
    10261 aatggttttg ggatagaact gggcaggaga cgtgaatggt ccatcgtaac agaaatctcg
    10321 gtaaatctcg tcgctaacca gtgcgatacc gtgcttgtct gccaa
//
LOCUS       DAOSUT010000146        10300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3610_length_10300_cov_9.041386, whole genome shotgun sequence.
ACCESSION   DAOSUT010000146 DAOSUT010000000
VERSION     DAOSUT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3610_length_10300_cov_9.041386"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..730)
                     /gene="folE"
                     /locus_tag="PLD05_12310"
     CDS             complement(128..730)
                     /gene="folE"
                     /locus_tag="PLD05_12310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOP78270.1"
                     /translation="MLHPKDKSVPVFPVDVQRIEHAVREILLAVGEDPDREGLRETPA
                     RVARMYAELFRGLKADPRRHLKKVFVEKYDEVVLVKDISFESICEHHLLPFMGKAHIA
                     YLPDGKVVGLSKLARVVEDISRRPQVQERMTEDIADLLMEELNAKGVTVIIEAIHTCM
                     TVRGIRKPGSLCVTSAVRGIFRTNASTRAEVMTLIYGAHR"
     gene            1174..2523
                     /locus_tag="PLD05_12315"
     CDS             1174..2523
                     /locus_tag="PLD05_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78271.1"
                     /translation="MKRRDFLKLGSAGVLMGTMPGYAVGLTRTKPRRIGLIGCGWYGK
                     SVLLRFLQVEPCEVVALADVDGQMLEGAANIVAERQSSHKKPKTYRDFRQMLAENEFD
                     IVQVSTPDHWHALAAIAAMKAGADVYCEKPISVDVVEGAAMVAAARKYNRVVQINTQR
                     RSTPHLIEARDRFIRENKLGKIAHVEIYCYYHMRFRGTSPDTDPPPHLDWDFWCGPAP
                     YRPYNPAIHPRRWRSFMEYGNGIVGDMCIHMLDMVRWMLDLGWPKRISSSGGILVQKD
                     SIANIPDTQTAVFDFGDVTVVWQHRTWGSTPNPDYPWGATFYGDQGTLHASVFRYDFI
                     PQGQGEKVHQDVQYELEQYPEDKTEKDLERHVAPAIRRHIRNFLDAVDARTRPVADVE
                     QGFISSASCILANLSLQLGRTLTWDAEHLQVVGDPEANQRLARPYRAPWEHPDPSKV"
     gene            2637..5444
                     /locus_tag="PLD05_12320"
     CDS             2637..5444
                     /locus_tag="PLD05_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOP78272.1"
                     /translation="MRSLSVKTRRSVCHQRTWAFTCLVMLSALGPWTSLWTQAQGSSA
                     EKLTFYVAESGNDQWTGRLPEPSSDKNNGPWASLVPLAEVLQKLAQERPSAPVEVVIR
                     PGRYELSEPIILDLSKMSLGQLTIRAERSRTAFLSGGRRITHWVPASAIGDLPPQLPA
                     ESRPHVFVADLKALGIHDYGSPGGGGIELYYQDSPMTLARWPNEGFVKIAEVLNEQPF
                     KTHGIPGDKMGKFVYEGTRQDRWQHEKDPWVHGYWFWDWSDQRHAIQSIDPERHLITV
                     KPPYHHYGYRKGQWYYGFNLLCELDAPGEWYLDRENGRLYFWPPDSAKTADELLAGQP
                     TVSCLTSLTDISNGRNVEISGLVLAHARGTAVRFVNCVDCQITDSHLYLLGGWGVTIQ
                     GGQGCRVSDCHLHLLGEGGVSATGGDRANLISAGHVIERNEIDHYGRIYPMYRAGVSI
                     SGVGQSVVHNHIHHAPHQAVSFSGNNHLIAFNEIDHVCLESNDAGAIYAGRDWTMRGT
                     QIVSNYFHDINGFENRGCMGVYLDDMFCGTVIRRNVFCRVTRAAFIGGGRDNLVENNI
                     FIECRPAVHVDARAMGWASYHVDTTMTERLKAMPYQSPLWQSAYPQLVNILQDEPAAP
                     KGNIIRHNVRVGGVWEHVEDKARPYLVMEKNLILPSEAISELFVDPQTENWQLKPDGK
                     VREELPEFESIDFEKIGLPRTGDGQSSEAGYRFIFVSQGEPTKEKQPPLPTRWEATIK
                     KMEDQDKEKAPPKDAVLFVGSSSIVRWDVAKWFPDLVTLNRGFGGSQIHEVTYYAERI
                     VIPYHPRLIVFYAGDNDIASGATPEEVLKRFQEFVTRVRASLPSTPIVFISIKPSLAR
                     WHLIESVRRANQLIQQYCRENPDLKLIFVDVDQAMIGPDGKPKPELFVKDGLHLSEDG
                     YRLWTELLRPVIDAQLKENRP"
     gene            complement(5448..8117)
                     /locus_tag="PLD05_12325"
     CDS             complement(5448..8117)
                     /locus_tag="PLD05_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78273.1"
                     /translation="MKCLLPFLLLLAIPNAWGQTERLYYPRQREDYIFLQGLRQRHLY
                     HLAECYCKDKLLRGDLSLPERAILTSELIVTLNEWGMETEDSNERQSRWQEAISVAEA
                     FYRRFPTSDWRFIVALMDLKYRITVASAIREEMQLGTAYTEAISDASFLDEIRSILRK
                     TINALKDREAELQEVFRRPKSGSAKDDGSVFQEQQWLSLEKHLQFELARGFAELARTY
                     PAKSEDHTAGMVEAARRLEVLSQLPLDHPLGVPSRLALARILRELGDGARSTDILKGL
                     AEEKLSLDERFALRVELIRLALATQQKEELDKLLAMGRLIEGKTAPQLDNAFLEAFLE
                     KWKNARANNSDEARAWEAKIREILNGLRQNGPAFWARRAELLVTQQIAGNQVHQNIDM
                     NVYLAENAYRAKRWEEALAAFDRARKAAEQQGNPVRALQLGLAAAAIEHERERHQEAW
                     RRYQDLYRSFSDQEGAKDAAELAIFHVGQLAKENPTEFLPLYQQELENYLRMWPQSEF
                     ACQAALYLGKIHNHFGHRIDAANYLLLSLESWLQHVASLEPQKRESAPIAPDTPSQSQ
                     TSQYSVIQTVSELETVTARILQDESQRPDTRQRLRELAQRLATWAKQARAHAPEEDWS
                     LRVATIATQLLLKGANDPHSADAVLEQFLPEPLDTIDHLDVTVKARSLSLKLECDAWL
                     EQSNDNAQIVRLMREIPIEARLTVLEDLGTRLSELPPQVRKRLALSLAHICEDLPKEW
                     SSVPGPRRPQLGLAYSYVLMLSDNESAALSWLRQLAAEFPDAADIQEEYALLLARQDR
                     MDLRQEALERFRAIAQGTPEGSPRWFRAKYMTAWLLIQLGQPERALEMIRVLEAIHPE
                     LGGDALRDRFRRLRALCENQITNSRPTSAGTAR"
     gene            complement(8114..8560)
                     /locus_tag="PLD05_12330"
     CDS             complement(8114..8560)
                     /locus_tag="PLD05_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP78274.1"
                     /translation="MKRPVAYHHASHLDLPMTPMIDVVFQLIIFFLCTTRFTPLEHVL
                     PMHLDLPGAKGAVTKVSPDLQDLTEIVIILEAHQDTVGISVNRRPVASMAELRSTLMT
                     LGKIRLDVPIVIDPSPEVRISRVIEVYDLCREIGFVNIQFATPADR"
     gene            complement(8592..9020)
                     /locus_tag="PLD05_12335"
     CDS             complement(8592..9020)
                     /locus_tag="PLD05_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOP78275.1"
                     /translation="MRTHGVRGIHQPKVRLNLTPMIDVTFQLILFFLLAGHMAQQESQ
                     SDLALPYARTGEKLVEGDTRRLVINVLANGQIVLSGESLSVERLVQVLASEAGAGRRV
                     EVRIRTDKETPYKFISPILLTCARAGLWNVSFAVISKEEE"
     gene            complement(9020..10021)
                     /locus_tag="PLD05_12340"
     CDS             complement(9020..10021)
                     /locus_tag="PLD05_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP78276.1"
                     /translation="MNTGESFCRVGPVVIGLCVLILSGTLAEPVTGIVPVAQLAAPAL
                     AAESSPFSPLPEPEAPSTPVGHTGGTSPPKTTAPNVLPQDGTRGQSPSTAAQSGASVE
                     IEREAKTSSSSLSFWRIVRAGGTVGFLIILLSIVAVALAVEHLLTLRRDLFMPLGLIE
                     EVSAKLSAKDWNGAAEACRAHPSVLAHILISGISEHGAGWNEMEKVMEETLAEQTARW
                     NRKVEYLSVIGNIAPMLGLLGTVIGMILAFRTVAETQGAARAADLAEAIYLALVTTVE
                     GLIVAIPSLAAYAFFRNRVDELMAEVAATVQKIFAPLKRRRATLAPAPAPPSAGASH"
BASE COUNT         2320 a         2709 c         2920 g         2351 t
ORIGIN      
        1 atagacgggc gaacgcaaga cctttccctt gatcaaccca gaaaggtctt cgtaaactcg
       61 gtctcgttgc tgatcgaggt agccggatgc catcgcctgt cggatctctt gtcgaacgat
      121 tacggcacta tcgatgggcg ccgtaaatga gggtcatcac ttcggcccga gttgaagcgt
      181 tggtgcgaaa gattccgcgt acggctgagg tgacgcacaa actccccggt tttcgaattc
      241 cccgcacggt catgcatgta tgaattgctt caatgatcac agtcacgccc ttagcgttga
      301 gttcctccat caataggtcg gcaatgtctt ccgtcattct ctcctgaact tgagggcgac
      361 gcgagatatc ctcgactacc cgcgccagtt tgcttaaccc aacaactttt ccatcaggaa
      421 gataggcgat atgggccttc cccatgaaag gaagcaaatg gtgctcgcaa atgctttcga
      481 agctaatatc cttaaccagg accacctcat catacttttc tacgaagacc tttttgagat
      541 ggcgccgcgg atcggccttt aacccccgaa aaagctccgc atacatcctg gcgaccctgg
      601 cgggagtttc tcgcaaccct tcgcggtccg gatcttcccc cacggcaaga agaatctctc
      661 gcactgcgtg ttcgattctt tgtacatcaa cgggaaacac aggcacggac ttgtccttgg
      721 gatgcagcat ctcgccgctg ttctcgctgg gcacctcctg gttcattgag tcagccattg
      781 tcattgttct acctcatccg attcatcgcc aatgtcactg ccaacttgta aattatacgt
      841 gaatcgcgcc gacaacaggc ctaagtgtgg tggtatctgt cctcattttg gggttggcag
      901 cgtggttttc tccagcgtct ttttctcgct tgccggaaag taaacaggcc aatgtggtat
      961 tggttgggac ctccggagtt atgtcgtatg ggcgtgacca ggaagatgtt ttaatggcgt
     1021 ggtttatttt gggtaaggca tgaggcataa tatccgaagc ccgtgggaag tcggcgggga
     1081 agatgttggg tttgtgtggc cagagttgtc tcggccgttg tcggtcaagt tttgagggag
     1141 ctctgtaaat cgagtgaaga taggagagtc atcatgaaac gacgggactt cctgaaattg
     1201 ggatcggcag gtgtgttgat ggggacaatg ccgggttacg ctgtgggctt aacccggacg
     1261 aaaccccggc gcattgggtt gatcggctgc ggctggtatg gaaaaagtgt tttgttgcga
     1321 ttcctccagg tggagccgtg tgaggtcgtg gcccttgcgg acgtcgatgg gcaaatgctc
     1381 gagggggcgg ccaatatcgt ggcggagcgg cagagttcgc acaagaagcc gaagacatat
     1441 cgagactttc gacagatgtt ggccgaaaac gagtttgaca tcgtccaagt cagtacgcct
     1501 gaccattggc acgccctggc ggcgattgct gccatgaagg cgggggccga tgtgtactgc
     1561 gaaaagccca tcagcgtgga tgtggtcgag ggcgcggcga tggtggctgc cgcccggaag
     1621 tacaaccgag tggttcaaat caacacccag cggcgaagta cgccgcactt gatcgaggca
     1681 agggatcgct tcattcgtga aaacaagttg ggcaagatcg cccatgtgga aatctattgc
     1741 tactaccaca tgcggttccg agggacctcg ccagataccg acccgccgcc ccatctcgat
     1801 tgggacttct ggtgtggacc ggctccgtat cgcccctaca atccggcgat ccatccgcgg
     1861 cgatggcgat catttatgga gtacggcaac gggatcgtgg gagatatgtg cattcatatg
     1921 ctcgacatgg tccgttggat gctcgaccta ggttggccca agcggatatc gtcttctggt
     1981 gggattttgg ttcagaagga cagtattgcc aacattccgg atacgcaaac cgcagtattt
     2041 gatttcggtg acgtgaccgt ggtttggcag catcgaacat ggggtagcac gcccaatccc
     2101 gactatccct ggggggctac cttctacggc gatcagggaa cactgcatgc cagcgtcttt
     2161 cgttacgatt tcatcccgca aggacaaggc gaaaaggtac accaggacgt ccaatatgaa
     2221 ttggaacagt atcctgaaga taagaccgaa aaggacctag aaaggcacgt cgccccggcg
     2281 attcgacggc acatccgcaa tttcctggat gctgtcgatg caagaacgcg acctgtcgcc
     2341 gacgtagagc aaggctttat ctcgtcggca tcctgtattt tagcaaacct gtccctgcaa
     2401 ttggggcgca cgctgacctg ggatgcagag cacttgcagg tggtcgggga tcccgaggcc
     2461 aatcagcgat tggctcgacc ctaccgcgcc ccgtgggaac atcccgatcc ttcaaaggtg
     2521 tgattccgat ttagctatca aaaccgttat taaaaaccca gggccacgtg atgataaggt
     2581 ggtcagatct ggggtcttgg ctattggtca gggtttcagt ctttgggaaa aatgccatga
     2641 gaagcctctc ggttaaaact cgtcggagcg tgtgtcacca gcgaacctgg gcatttacgt
     2701 gccttgtaat gctttctgcc cttggacctt ggacatccct ttggactcaa gcccagggta
     2761 gcagcgcaga gaagcttacc ttttatgtgg cggaaagcgg caacgaccaa tggacaggcc
     2821 gcctgccaga accgagttcg gacaagaata atggtccctg ggcatccttg gtgccactgg
     2881 ccgaggtact tcaaaagctc gcccaagaga gaccatccgc accggtcgag gttgtgattc
     2941 gtccaggccg gtatgagctt tctgagccca ttatccttga cttgtccaag atgtcgctcg
     3001 gccaattgac gattcgtgcg gagcgatcga gaacggcctt tctgagcggt ggacgacgta
     3061 tcacccactg ggttccagca tcggctatcg gtgatttgcc acctcaattg ccggccgagt
     3121 ctcggcccca cgtatttgtg gcggatctga aagcgttggg catccatgac tacggatcgc
     3181 cgggcggtgg ggggatcgag ctttactacc aggatagtcc gatgaccttg gcccggtggc
     3241 caaatgaggg atttgtaaag atcgctgagg tcctaaacga acagcccttc aaaacccatg
     3301 gcatcccggg tgataaaatg ggaaagtttg tctatgaggg gacacgccaa gatcgatggc
     3361 aacacgaaaa ggatccctgg gtccacggtt attggttttg ggattggtca gaccagcggc
     3421 acgccattca gtcgatcgat ccggaacggc atctcatcac ggtgaagcct ccttaccacc
     3481 attacggtta tcgcaaaggc caatggtatt acggatttaa cctgctgtgt gaactagacg
     3541 cgccggggga atggtatctc gatcgggaga acggaaggct ctacttctgg ccgccagact
     3601 ccgcaaagac agcggacgaa ctattggccg gtcagcccac agtatcctgt ttgacttcgc
     3661 tgacagacat ttcgaacggt cgtaacgtag agatatcagg ccttgtgttg gctcacgcgc
     3721 ggggaacggc tgtccggttt gtgaattgcg ttgactgcca gataacggat tcgcatctct
     3781 acttgttagg cggctgggga gtcacgattc agggcggtca aggctgtcgc gtgagtgatt
     3841 gccatctgca tctgttgggg gaaggcggag tttcggcgac tggcggcgat cgtgcaaatc
     3901 ttatttcagc cggccatgtt attgaacgaa atgagattga ccattatggg cggatttacc
     3961 ctatgtatcg tgccggcgtg tctattagtg gagttgggca atccgtggtc cacaatcaca
     4021 ttcaccacgc cccacaccag gcagtaagct tttcaggcaa caatcatttg attgccttca
     4081 atgagatcga tcacgtctgc ttggaatcga acgatgccgg agcgatctac gcgggccgag
     4141 attggacgat gcgcggcacg caaatcgttt ctaattactt ccacgacatc aatggttttg
     4201 agaatcgtgg ctgcatgggg gtttacttgg atgacatgtt ttgtgggacg gtgattcgca
     4261 ggaatgtttt ttgccgcgtg acccgtgcag catttatcgg gggtggccgg gataacctgg
     4321 tggaaaacaa catctttatt gagtgtcgcc cggcggtcca tgtggatgcc cgcgctatgg
     4381 gatgggcatc gtatcacgtg gacaccacga tgaccgagcg attgaaggct atgccttatc
     4441 aaagtcccct gtggcaatcg gcttatcctc agcttgtcaa cattcttcag gatgagccgg
     4501 cggctcccaa aggcaacatt attcgccata acgttcgtgt gggtggagtt tgggagcacg
     4561 tcgaagacaa ggctcgcccc tatttggtga tggagaaaaa cctgattctc ccgagtgagg
     4621 ccatctctga gctgtttgtc gacccgcaga cagagaactg gcagctcaag cccgatggca
     4681 aagtaagaga agagcttcca gaattcgagt ccattgattt tgaaaagatc ggcttgccga
     4741 gaaccgggga tggacaatcg agcgaggcag ggtatcgatt tattttcgta tcgcaagggg
     4801 agcccacaaa ggagaagcaa ccgcctttgc ccacccgctg ggaggcgact atcaagaaga
     4861 tggaggatca agacaaggag aaagccccgc ctaaggatgc cgtccttttt gtgggaagtt
     4921 cgagcatcgt tcgctgggat gttgccaagt ggtttcccga tttagttacg ttgaaccgcg
     4981 gctttggtgg ctctcaaatc catgaggtta cctattacgc agagaggatc gtgatcccgt
     5041 atcacccgcg gctgattgtc ttttatgcgg gagataacga cattgccagc ggggcaactc
     5101 ccgaagaggt cctgaaacgc ttccaagaat tcgtcacaag ggttcgggcc agtctcccat
     5161 caacgccaat tgtttttatt tccatcaaac cgtccctggc gcgttggcat ctcatcgagt
     5221 ccgtccgtcg ggccaatcaa ttgattcagc agtattgtcg ggaaaacccg gacctcaagt
     5281 taatcttcgt cgatgtggat caagccatga ttgggccaga tggaaaaccg aagcccgagt
     5341 tgtttgtcaa ggacggcctc catctgagtg aagacggata tcgcctatgg acggaattac
     5401 tgcgaccggt cattgatgcc caattgaaag aaaatcggcc ctgattacta tcgggcggtg
     5461 cctgccgatg ttggacgaga attggtgatc tggttttcgc aaagggcccg cagccggcga
     5521 aatcggtcac gcagcgcgtc accaccaagt tctggatgaa tcgcttccaa aacacgaatc
     5581 atctcgagag ctcgttccgg ctggccaagc tgaatgagaa gccaggccgt catgtactta
     5641 gcgcgaaacc agcgcggtga gccttcgggc gttccctggg caatggcgcg aaagcgctct
     5701 aaggcttcct gccgtaaatc cattcggtct tgtcgggcca ggagaagggc gtattcttct
     5761 tgaatatcgg cagcgtcggg gaactctgcg gcaagttggc gaagccatga gagggccgct
     5821 gactcgttgt cggatagcat taacacgtaa ctataggcca gccccaactg tgggcgacgg
     5881 ggtccgggaa cggaggacca ctcttttggc aagtcctcgc agatgtgggc gagggagagt
     5941 gccagcctct tccgaacttg gggcgggagc tccgaaagcc gcgtcccgag atcctccaaa
     6001 acggtcaaac gggcctcaat cggtatttca cgcatgaggc gaacaatttg tgcattatcg
     6061 ttcgactgct caagccaggc atcgcattct aatttaaggc tgagggatct cgccttgacc
     6121 gtgacatcga ggtgatcaat agtatcgagt ggttcgggca aaaattgttc gagaactgcg
     6181 tcggccgagt gaggatcgtt tgcgcctttt agcagcaact gtgtcgcgat ggtcgccacc
     6241 cgcaggctcc aatcctcttc aggtgcgtgt gctcttgcct gtttcgccca ggttgctaaa
     6301 cgttgggcga gttctcgtag tcgctgtctt gtgtcgggcc gctgagactc atcctgaaga
     6361 attcgcgccg tgacggtttc caattccgaa acggtttgaa tgacagaata ttggcttgtc
     6421 tgcgattgcg atggagtgtc cggggcgatc ggcgccgatt ctcgtttttg gggttcgagg
     6481 gacgcaacgt gttgtaacca agattctagg gataatagaa gatagttggc tgcatcgatc
     6541 cggtggccaa aatggttatg aattttgccc agataaagag cagcctgaca cgcgaactcc
     6601 gattggggcc acatccgaag atagttctcg agctcctgtt gataaagcgg cagaaactcg
     6661 gtgggattct ctttggccaa ttgccccaca tgaaaaatag cgagctctgc tgcgtcctta
     6721 gccccttctt gatccgagaa actccgataa aggtcctggt agcgccgcca agcctcttga
     6781 tgccgttccc tctcatgttc aatggccgcc gcggccaggc ctaactgcag agcgcgaacg
     6841 gggtttccct gttgctcggc agccttgcgc gcccggtcga aagcggccag ggcttcctcc
     6901 caccgcttgg cacgataggc attttcggca agatagacat tcatgtcgat gttctggtga
     6961 acttggttcc ccgcaatctg ctgcgtcacc aacaactcgg cccgtcgcgc ccaaaacgcg
     7021 ggaccatttt gccgcaagcc attgagaatc tctcgaatct tggcctccca ggcgcgggct
     7081 tcatccgagt tgttcgcgcg agcgtttttc catttttcga gaaaagcctc aagaaatgcg
     7141 ttatcgagtt gtggggcggt ctttccctca atgagccgac ccatggcgag aagtttatcc
     7201 aactcctcct tttgctgagt cgccagggcc agacgaataa gttcgactcg gagggcaaat
     7261 cgctcgtcaa gcgataactt ctcttccgca aggcccttta agatatcagt gctcctcgcg
     7321 ccgtcgccca actcgcgaag aatcctcgct agcgccaaac gggatgggac acccagcgga
     7381 tggtcgagcg gcaactgcga aaggacttca agccgtcgag ccgcctccac catgcccgcg
     7441 gtatggtcct cactcttcgc gggataggtt cgcgctaact ctgcaaatcc gcgagctaat
     7501 tcgaactgca ggtgtttttc cagacttaac cattgttgct cctgaaagac ggagccatca
     7561 tcctttgccg acccactctt gggacgacgg aatacctcct gcaattcggc ttcgcggtct
     7621 tttagggcgt tgatcgtctt ccgtaaaatc gaacggattt cgtcaaggaa agacgcgtct
     7681 gaaatggctt cggtataagc agttcccagt tgcatttcct cgcggatcgc gctggccact
     7741 gttatccgat atttcaaatc catgagcgct acgataaatc gccaatcgga tgtaggaaaa
     7801 cggcgataaa acgcttcggc aacgctaatg gcctcctgcc aacgggattg acgttcgttc
     7861 gagtcttcgg tttccatgcc ccactcgttc agggtcacga tcaattcaga tgtgagaatg
     7921 gcccgctcag gaagggaaag atcgcccctt agcagcttgt ccttgcagta acactcggcc
     7981 aaatggtaga gatgacgctg ccggagacct tgaaggaaaa tgtaatcctc ccgttgccgc
     8041 gggtagtaca accgctctgt ctgcccccac gcattgggaa tggccagcaa aaggagaaaa
     8101 ggaagcaagc atttcatctg tcggctgggg tcgcaaattg gatattgacg aacccgattt
     8161 ctcggcacag gtcataaacc tcaatgactc gggaaatgcg gacctcagga gagggatcga
     8221 tcacgatggg aacgtctaac cgaattttac caagtgtcat cagtgtggaa cgcaattccg
     8281 ccatgctggc aaccggacga cgattgacac tgattcccac cgtgtcctgg tgagcttcta
     8341 aaatgatgac gatctccgtc aagtcttgca gatcgggact gaccttggtc accgcccctt
     8401 tggccccggg taagtcaaga tgcatgggaa gaacatgctc caagggggtg aatcgagtgg
     8461 tgcaaaggaa aaagataatg agctggaaca cgacgtcgat catcggcgtc atgggcaaat
     8521 ccaggtggga agcatgatgg tacgcgacgg gtcgcttcat gattgatcga atttcacggg
     8581 ccaaccggta attactcttc ttcttttgag atcactgcga aggacacatt ccaaaggcct
     8641 gcccgggcgc atgtcagcaa aattggcgaa ataaacttgt acggcgtctc tttgtcagta
     8701 cgaattcgca cctctactct tcgtccggcg ccggcctcgg atgcaagaac ttgaaccagc
     8761 cgttccaccg agagggattc tccggatagg acgatttggc cgttcgcaag gacgttgatc
     8821 accaaccgcc tggtgtcgcc ttctacaagc ttttcaccgg ttcgggcata aggcagtgcc
     8881 aggtcgctct gactttcctg ctgggccata tgtccagcaa ggaggaaaaa caaaatgagc
     8941 tgaaatgtca cgtcgatcat cggcgtgagg tttaaacgca ccttgggctg gtgtattcct
     9001 cgaacaccgt gcgtccgcat caatggcttg ctcctgccga ggggggagcg ggggccggcg
     9061 ccaacgtggc acgacgccgt ttgagtgggg caaagatttt ctggactgta gccgccactt
     9121 ccgccataag ttcatccacc cgattacgga aaaatgcgta ggccgccagc gacggaatgg
     9181 ccacaatgag gccttccacc gtggtcacca aggcgagata aatagcctcc gctaagtccg
     9241 ccgccctcgc cgcaccctgc gtttcggcaa ctgtgcggaa cgccagaatc atgccgatga
     9301 ccgtgcctaa aagtccgagc atcggtgcga tgttcccgat gacggagagg tattcaactt
     9361 tccggttcca acgggcggtc tgttcggcca gggtctcttc catgaccttt tccatctcgt
     9421 tccagcctgc cccatgctcc gagatccccg atataaggat atgtgccagc accgaggggt
     9481 gggctcggca cgcctcagcg gcaccattcc agtcttttgc tgaaagcttg gcagagacct
     9541 cctcgatcaa gccgagcggc atgaaaaggt cccgccgcaa cgtcaagaga tgctcaaccg
     9601 ccaacgccac tgcgacaatc gacagcaaaa tgatcaggaa tcctacggtt ccaccggctc
     9661 gcacaatccg ccagaacgaa agcgaagaag aactagtctt ggcctcccgc tctatttcga
     9721 cgctcgcacc actttgggcg gctgtggagg gtgattggcc cctggtgccg tcctggggta
     9781 gcacattcgg agccgtggtt tttgggggac tcgtaccccc agtgtggcca acgggagttg
     9841 aaggggcctc aggctcaggc aacggcgaaa aaggtgacga ttccgcggca agggctggag
     9901 ctgcgagttg tgccacgggg acgatcccgg tgacgggttc ggccagtgtg ccggagagga
     9961 tgaggacgca taaaccgatg acgacgggcc caacccggca aaatgattcg cccgtgttca
    10021 caagtgttaa aaacctttgc ccaaaggaaa ccatcgacca gtggcctttg tttcgaaaat
    10081 catgaacggc aagtgactgc gtaaatcaca caagaggcgt aaatgattcg ggaatacgcc
    10141 ttaccactta tggtagcaat atcctgaccg caggttgagg ccaatcagct cataacccgt
    10201 gtgtagtgtc acgacgaggc aaggacgtct tcgatccggc tgattgcact gccttgggca
    10261 aagcgggttt tgatctgctg ccctgggcga agggccgacg
//
LOCUS       DAOSUT010000147         9991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3715_length_9991_cov_9.494263, whole genome shotgun sequence.
ACCESSION   DAOSUT010000147 DAOSUT010000000
VERSION     DAOSUT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3715_length_9991_cov_9.494263"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..1512
                     /locus_tag="PLD05_12345"
     CDS             409..1512
                     /locus_tag="PLD05_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP78277.1"
                     /translation="MSEINRRDFVRTGITAAAGAALGLQPTFTVKAGNPENFDTSKIL
                     NYNPDMEYRRCGKTNLMISAVCLGGHWKRLNTVVPGSFQGGNWLGAKIDEEDFQKNRY
                     DVVTRCIERGINYIDACTGQEVMAYARALKGRRDKMYLGYSWYQRESRFAEWRSVEKL
                     KESLDMGLKEAGLEYVDLWRITLHEQSSRHSEAEIEAVIAALDWAKKTGRARFTGISS
                     HDRPHIKMLIEKYPEQLEVICTPYTAKTKVVQDETGLWAAMQKHDVGWFGIKPFASGS
                     LFKGDSSPNSPYREEDDRLARLAIRYILCNPAITAPIPGMITPEQVDNVALAVKERRE
                     LDLEEKALLDREMERAWANLPYHYQWLKDWEYV"
     gene            complement(1575..1892)
                     /locus_tag="PLD05_12350"
     CDS             complement(1575..1892)
                     /locus_tag="PLD05_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOP78278.1"
                     /translation="MAIREETVWEALKQVRDPEIGIDLVNLGLIYEILITEHEGKSDI
                     HVKMTLTSPACPSGPQMIQEVKDVLRQLGDEVGQVNVEVVLSPPWTPDRMSEEARDEL
                     GFY"
     gene            complement(1929..2249)
                     /locus_tag="PLD05_12355"
     CDS             complement(1929..2249)
                     /locus_tag="PLD05_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOP78279.1"
                     /translation="MGELIRVCRVSDLPDPGKAVFDVSGRMIALFHVSGTFWALDDMC
                     THDGGPLAEGELHGYTIVCPRHGAQFDIRTGQAKTMPAVRATTAHKVVVKGDEVYVEL
                     NEES"
     gene            complement(2299..3621)
                     /gene="sufD"
                     /locus_tag="PLD05_12360"
     CDS             complement(2299..3621)
                     /gene="sufD"
                     /locus_tag="PLD05_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOP78280.1"
                     /translation="MMMPSLNLDTSFTDEGFHNVAFHRPQPNWLKELRQRAYRTFEDL
                     PMPNRSMEEWRRTELRHFRLLQFQAWSPHPEQAPEIPSQLIPRLDTDGLIITLNGRKL
                     ESRLDPALGQQGVVLNCLEDSILNGWEELIRKHLFSILDWKYDKFAAMHAAFWTGGIF
                     VYVPKDIRVNKPLLHVAALMPGQADVGHTLIILDEGAEATVVSETVSVDPSASSLYCG
                     GIEIILRPGARLRYVNMQNWNQATFHFAHQKAVLAERASLEWTLGALGGRLAKVNQHL
                     VLDGPESEGIVNGVMFAENRQHLAYHTLQHHRTPKTKSDLLYKGALQDQSHLVWRGMI
                     RVEPGAVKADGYQRNDNLMLSETARTDCIPGLEILADDVRCTHGATAGRVDEEEIFYC
                     QTRGLTRREATRLVVTGFFQQVLDRITIDSVREALTDIIQHRVREYRG"
     gene            complement(3778..5178)
                     /gene="sufB"
                     /locus_tag="PLD05_12365"
     CDS             complement(3778..5178)
                     /gene="sufB"
                     /locus_tag="PLD05_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOP78281.1"
                     /translation="MMAEKVDVIGEINKYDFRVDERYVFKAQKGLNRDIVAEISAMKN
                     EPQWMRDFRLKALEIFESKPMPTWGGNIDIDFQDIYYYIKPTERPGHSWEELPEEIKK
                     TFDRLGIPEAEKRFLAGVKAQYESEVVYGSLREDLAKRGVIFVDMDTAVREYPDLVRQ
                     YFSTVIPPTDNKFAALNSAVWSGGSFIYVPPGVNVEFPLQAYFRINAAQMGQFERTLI
                     IVDEGASCHYVEGCTAPMYSSESLHAAVVEVIAKRGARVRYTTIQNWANNIYNLVTKR
                     AVAYADALVEWVDGNLGSRLTMKYPAVYMLEPGARAEILSVAFASRGQHQDAGAKVIH
                     AAPHTSSRIVSKSISKSGGRSSYRGLVKIERGAKKSKSNVVCDALILDPQSRSDTYPY
                     IELEEQDVMIGHEASVSKIGEEQLFYLCSRGLTEAEASTMIVAGFIEPLVKELPMEYA
                     VEMNRLIALQMEGSVG"
     gene            complement(5297..6112)
                     /gene="sufC"
                     /locus_tag="PLD05_12370"
     CDS             complement(5297..6112)
                     /gene="sufC"
                     /locus_tag="PLD05_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOP78282.1"
                     /translation="MSPETLSVIDLHVSVEGKPILKGVNLTIRRGEIHALMGPNGSGK
                     TTLGLAIMGHPRYEITRGRIELDGEDITQLEPHERARRGLFMAFQRPVAIPGVRLADF
                     LRHAVSNVRNPARKEGEELIPMREFRKELKEKMDLLRMDAEFARRYVNDGFSGGEMKR
                     AEILQMAMLRPKFAILDETDSGLDVDAVRLASQSIAEIGGRQMGLLIITHHERLLEHN
                     PPDVTHVLLGGRIVETGGPELAERLHREGYDQLRQKYPEAAAEEAALAGQGKQ"
     gene            complement(6133..6735)
                     /locus_tag="PLD05_12375"
     CDS             complement(6133..6735)
                     /locus_tag="PLD05_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOP78283.1"
                     /translation="MTQPWIEGRFEEGLVITTVEQAINWARQGSLWPLTFGLACCAIE
                     MMATGAGRYDIDRFGAGAFRGTPRQADLMIVAGTVTFKMASRVRRLYEMMPDPKYVIA
                     MGACTLGGGPYFEWGYHVVKGVDLIVPVDVYVPGCPPRPEALLEGLMRLQEKIASRKI
                     TKPPASGFEERLPLPQHTGYVDVPESALVFHHQKVKKGSA"
     gene            complement(6809..7567)
                     /locus_tag="PLD05_12380"
     CDS             complement(6809..7567)
                     /locus_tag="PLD05_12380"
                     /inference="COORDINATES: protein motif:HMM:NF025186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOP78284.1"
                     /translation="MGVPQVQSDLDILDCLRNAGPMTVAELAERFSVTPTAIRQRLNR
                     LHAEGLIERQASRAGRGRPHYVYGITGKGRQTAGANYTDLAIALWGAIRSIDDPKVRA
                     HVLTAAAHEMARMYWRQVKGLTVSERMAEVGRLLSQKRIPFSVEATGETGSGSNLQAT
                     SRPSVASPGDATASRGTRLPILVGQACPYPDLAEIDGQICQWERLWLSELLGIEVHLT
                     QCRREGGSECRFQAAENICSDSDNRWERVQAAST"
     gene            8027..8530
                     /gene="ispF"
                     /locus_tag="PLD05_12385"
     CDS             8027..8530
                     /gene="ispF"
                     /locus_tag="PLD05_12385"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOP78285.1"
                     /translation="MAQTFRVGLGTDLHRLVEPGPLILGNIAIPFEKHALGHSDADAL
                     IHAMIDAILGASGGGDIGQLFPDTDEANRGRDSGEMLSIVWGRVRAQGWALGNVDCVV
                     HLQRPKLAPYIPRMVERLSELMAVPPETISIKAKTGEGIGLVGREEAVAAECVVLLVR
                     ETDSSCQ"
     gene            8629..>9991
                     /gene="cysS"
                     /locus_tag="PLD05_12390"
     CDS             8629..>9991
                     /gene="cysS"
                     /locus_tag="PLD05_12390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOP78286.1"
                     /translation="MIRVYNTLTRKKEVFEPVQPGKVGIYLCGPTVYKPSHIGHMVGP
                     IIFDAIKRYLTYNGYKVTWVVNITDVDDKLIAESQRRGIPMSELAEEMIADYMANLQA
                     LGIDGIDHFPRATENIDAIITFTEDLIRKGYAYPAGGDVYFDVSKVPDYGKLSRRKLE
                     SMLPEGGEGVERKRSPYDFALWKGAKPNEPFWNSPWGPGRPGWHIECSVMSSRLLGET
                     FDIHGGGLDLIFPHHENEIAQSEARHGKPMVRYWMHNGLMQAADEIGKVGGRNTRVAE
                     DDLDAQQAGKISKSKGASPFRDLLKEYRPETIRLFILSTHYRRPIEFSERRLQDTETA
                     IETFYRFFERFERITRQSFYSLPVPKSRKEGDLLDGNDAFMQDVAGHRARFLEFMDDD
                     FNTGGAIGELFELVRRLNRFVDEHKLEDPQHRTPEKTAALVRGATVLRELGHVLGLFR
                     QPPP"
BASE COUNT         2227 a         2900 c         2655 g         2209 t
ORIGIN      
        1 acggtcgggt tcacggcgga gaagcgtcag ttgtctcagc ccatattctc ccctcgcccg
       61 ttcggcggga gaggggtcgg gggtgagggt ggctcgagtc gctggcggtt ttctcaatcg
      121 attccgacgt tcgcttgccc tcaccccaac cctctcccag agggagaagg ggactttttg
      181 ggataggctc ttaaaaaaat aacggcccaa tccgcgctaa ggcataacta tcgctcataa
      241 ccgatctaga aaatccaacg tgccgtcgat ggcgtgaagt gaagttctgt tgccaagcgg
      301 gttgcggcgc atcataatga tgaccagttg cggatgctct ggtgccgagg ataacttggt
      361 tgggagagcg tggtttcgcg gaaatccttt tttcggggag acaaagccat gtcagagatc
      421 aatcggcggg attttgttcg gacagggatc acggccgcgg cgggagcggc cctcggcctt
      481 cagccgactt tcacagttaa ggcaggcaac cctgagaact tcgacacaag caagatcctc
      541 aattacaacc cggacatgga gtatcgccgt tgtggcaaga ccaacttgat gatctcggcg
      601 gtttgtctcg gtgggcactg gaagcggctc aacacggtcg tccccggctc gtttcaaggg
      661 ggcaactggt tgggggccaa gatcgacgaa gaggatttcc aaaagaaccg ttatgatgtg
      721 gtgacccgtt gtatcgagcg cgggatcaat tacattgacg catgcacagg gcaagaggtg
      781 atggcctatg cccgtgctct caaaggtcgt cgggataaga tgtacctcgg ctattcctgg
      841 tatcagcggg aatcccgatt cgccgaatgg cgaagtgtcg agaaacttaa ggaaagcctg
      901 gatatgggac tgaaggaggc gggcttggag tacgtcgatt tgtggcgtat caccctccac
      961 gaacaaagca gccggcacag tgaagccgag atcgaggccg tcattgccgc ccttgattgg
     1021 gccaaaaaga caggccgggc acgtttcacc ggcatatcgt ctcacgaccg cccgcacatc
     1081 aaaatgctga tcgaaaagta tcccgaacaa ttggaagtaa tctgcacgcc ctacacggcc
     1141 aagacaaagg tcgttcaaga tgagacaggg ctgtgggccg cgatgcaaaa gcacgatgtg
     1201 gggtggtttg ggattaagcc tttcgccagc ggctcgctgt tcaagggcga cagctcgccc
     1261 aacagtcctt accgcgagga ggacgaccgc ctggcccggc tggcgattcg ctatatcttg
     1321 tgcaatccgg cgatcacggc accgatccca ggcatgatca cgccggaaca agtcgataat
     1381 gtagcattgg ccgtcaagga acggcgcgag ctagacctgg aagagaaggc acttctcgac
     1441 cgcgaaatgg aacgggcctg ggcgaacctg ccctatcact atcagtggct taaagactgg
     1501 gaatatgtgt aatacgccgg ctgtttcaag gcctgtcatt tcgtaaccgc aggccattgc
     1561 cgcgatctgg catgtcaata gaagcccagc tcgtcacggg cttcttcaga catgcggtcc
     1621 ggcgtccatg gcggcgataa gactacctcg acgttgacct ggccgacctc gtccccgagt
     1681 tggcggagga cgtctttcac ttcctgaatc atctgtggac cagagggaca cgcgggactg
     1741 gtcaaggtca tttttacgtg gatatccgac ttgccctcgt gctctgtgat caagatctcg
     1801 tagatcaacc ccaggtttac caggtcgatc ccaatttccg gatcacgaac ctgtttgagt
     1861 gcttcccaaa cggtctcttc tcgaatagcc acggaaggtt ctccagattc cccttgcaag
     1921 ccagcctttc aactctcctc gttcagctcg acatacacct catcgccttt gacgacaacc
     1981 ttatgggcag tcgtggccct gacggccggc atcgttttgg cctggccggt gcgaatatca
     2041 aactgcgccc catggcgagg acaaacaatc gtataaccgt gaagttcgcc ttccgccagt
     2101 ggtccaccgt cgtgcgtgca catatcatca agggcccaaa atgtcccgga aacatggaac
     2161 agggcgatca ttcgaccact tacatcgaaa acggcctttc ccgggtcagg caagtccgaa
     2221 acacgacaaa cacgaatcaa ctcgcccatt ttgtcttcct tttctgccac cgtgggcaac
     2281 agtcttcaat cttgcggctt aaccgcgata ttcccggaca cggtgctgga tgatgtcggt
     2341 cagggcttct cggacactgt cgatggtaat ccgatctaag acctgctgga agaagccggt
     2401 gacgaccaac cgggtggcct cacgccgagt aagaccgcgc gtttggcaat agaaaatctc
     2461 ctcttcatcc acgcgaccgg ctgtcgcccc gtgtgtgcat cgcacatcgt cggccaaaat
     2521 ctccaatccg gggatgcaat ccgtcctggc ggtctcgctg agcatcaaat tgtcgttccg
     2581 ttggtaaccg tcggctttca cagcccccgg ttccacgcga atcatgcccc gccaaacgag
     2641 atgggactga tcttggaggg cgcccttgta aaggagatca ctcttagtct tcggcgtccg
     2701 gtgatgttgc agcgtatgat atgccaaatg ttggcgattc tccgcaaaca tcacgccatt
     2761 gacgatgcct tcactttcgg gaccatccag gacgagatgc tgattaacct tggccaatcg
     2821 cccaccaagg gcacctaggg tccactccag cgaggcccgc tccgccagta cggccttttg
     2881 atgcgcaaag tggaaggttg cctgattcca attttgcatg ttgacgtagc gtaaccgtgc
     2941 ccccggacgg aggatgatct caattccgcc gcaatatagg ctacttgccg agggatcgac
     3001 actgaccgtc tcgctgacca ccgtggcttc agcgccctca tccaggatga tcagtgtgtg
     3061 tcccacatca gcttgaccag gcatcagggc tgcgacatgc aggagaggtt tattcaccct
     3121 tatgtctttg ggcacataga cgaatatacc gcctgtccaa aaggcagcat gcatggcggc
     3181 aaacttgtcg tatttccaat ccaggatgga gaagaggtgt ttacggatca actcctccca
     3241 accgttaaga atcgagtcct cgagacaatt cagcacaaca ccctgttgcc caagcgcggg
     3301 atcgagccgg ctttcaagct ttcgcccatt gagcgtgatg atcaacccgt ccgtatcaag
     3361 tcgaggtata agttgggaag gaatctcagg agcttgctcc ggatggggag accacgcttg
     3421 aaattgaagc aggcgaaagt gccgcagttc cgttcgtcgc cattcctcca tggagcgatt
     3481 cggcatcggc aaatcttcaa acgtgcgata ggcccgctgc cgaagctctt tgagccagtt
     3541 gggttgtggt cgatggaacg caacgttgtg gaatccttcg tcggtgaagc tggtgtcaag
     3601 gttaagagat ggcatcatca tcgctttgac taaacggtcc tgctgggata gaacccattc
     3661 ttttggaaag ttatttgtaa cacttggctg cttccgccaa acacccgtgt cccctctgcc
     3721 acatcgcgtt cccacactgc cccgtgaggg attactccaa acgtcgccgt aagacgatca
     3781 gccgacactc ccttccatct ggagggcaat caaccggttc atttcgacgg cgtactccat
     3841 gggcaattcc ttgaccaagg gctcgatgaa ccccgcaacg atcattgtgc tggcctcggc
     3901 ctccgtcagg ccacggctgc acagataaaa gagctgctct tccccgatct tggacacact
     3961 tgcctcgtgc ccaatcatca catcttgttc ctcaagttca atataaggat acgtatcgct
     4021 gcgactctgt gggtccaaaa tcagggcatc acagaccacg ttcgatttag attttttcgc
     4081 gccgcgctcg atcttgacca agccacgata gcttgaccgc ccaccggact tggagatcga
     4141 cttggacacg atgcgactgg acgtgtgtgg ggcggcgtgg ataaccttgg ccccggcgtc
     4201 ctgatgttgt ccccttgagg cgaaggctac cgagaggatc tctgcccggg ctccaggttc
     4261 cagcatgtac actgctggat atttcatcgt caatcggctg cccaagtttc catccaccca
     4321 ttccaccagg gcatctgcgt aagctacagc ccgcttcgtg accagattgt aaatattatt
     4381 ggcccaattc tggattgtgg tgtaacgcac gcgggccccc cgtttcgcaa tcacctcgac
     4441 caccgcggcg tggagacttt ccgaactgta catcggggcc gtacaacctt ccacgtagtg
     4501 gcaagaggcc ccttcatcga cgatgattaa cgttcgctca aactgcccca tctgcgcggc
     4561 gttgatccga aaataggctt gaagtggaaa ttccacattc acccccggcg ggacgtaaat
     4621 gaacgagcca ccagaccaca ccgccgaatt caacgctgcg aatttgttat ccgtaggcgg
     4681 aatgaccgtc gaaaagtatt gccgcaccaa atccgggtac tcgcggacgg cagtatccat
     4741 atccacgaag ataacaccgc gcttggccag gtcttcgcgc aacgacccat agacgacctc
     4801 gctttcgtat tgggccttga cccccgccaa aaatcgcttt tcggcctccg gaatgccgag
     4861 tcggtcaaat gttttcttga tctcttccgg caattcttcc cacgaatgtc ctggccgctc
     4921 tgtcggctta atgtagtagt agatatcttg gaaatcgatg tcgatatttc cgccccaggt
     4981 tggcatgggt ttcgattcaa agatttcgag ggccttcagg cggaaatccc gcatccactg
     5041 cggttcgttc ttcatggccg aaatttcggc cacaatgtcg cgattgaggc ctttttgggc
     5101 cttgaagaca tatcgctcat cgacccgaaa gtcgtactta ttgatttcgc cgatcacatc
     5161 gactttttca gccatcatta tctctccgaa agcctagctc ccggcctgct tctcaaggcc
     5221 ggtccctccg atgtcgtact ggcatccagc cggaccgtcg catcacacgc tcatgggttg
     5281 ttaacatcac gggaagtcac tgttttccct ggcccgccaa tgccgcttct tccgcagcgg
     5341 cctcagggta tttctggcga agttgatcgt aaccctcacg atggagtcgt tccgccaact
     5401 ctggaccgcc cgtctcgaca attcgcccac ccaaaaggac atgcgttaca tcgggagggt
     5461 tgtgttccaa tagacgttca tggtgggtga tgatcaagag ccccatctgc cgcccaccga
     5521 tctcggcaat gctttggctg gccagccgaa ctgcatcgac gtccaaaccg ctgtcggttt
     5581 cgtccaagat ggcgaacttg ggacgcaaca tcgccatttg caggatttcg gctcgtttca
     5641 tctcaccgcc ggaaaaacca tcgttgacgt agcggcgggc aaactccgca tccatccgca
     5701 gcaaatccat tttctccttc aattccttgc ggaactcccg catcggaatg agctcctcac
     5761 cttccttacg ggccggatta cgcacgttcg acaccgcatg acgcaggaaa tcggccaacc
     5821 gcacacccgg tatcgccacg ggacgctgaa atgccatgaa taacccacgc cgcgcgcgtt
     5881 catgcggttc caactgagtg atgtcttcac catccaactc gatgcggccg cgggtgatct
     5941 cgtaccgcgg gtggcccata atggctaggc caagcgtcgt cttgcccgaa ccattgggcc
     6001 ccatcagggc gtggatctca ccgcggcgaa tggtcaaatt cacccctttc aggatgggct
     6061 ttccctcaac actgacatgc aaatcgataa cagaaagggt ttccggactc atctttgcct
     6121 ttcccaaaag atctatgctg aacctttttt gactttttga tgatggaaga ccagtgcgct
     6181 ctccggaaca tccacgtaac ctgtatgctg cggcagcgga agcctttcct caaaaccgct
     6241 cgcgggcggc ttcgtgatct tccgcgacgc gatcttctcc tgaagccgca tcaatccctc
     6301 caaaagggct tcgggacgtg gcggacaacc cggaacataa acatccaccg gcacaatcag
     6361 gtccacacct tttactacgt ggtatcccca ctcaaagtac ggtcccccac caagtgtaca
     6421 tgcccccatg gcgatgacat attttgggtc gggcatcatt tcgtagagcc gccggacacg
     6481 gctggccatc ttgaacgtca ccgtccccgc cacaatcatc aagtcagcct ggcgcggcgt
     6541 cccacgaaag gcccccgccc caaaccgatc aatatcgtag cggcctgccc cagttgccat
     6601 catttcgatg gcgcaacagg ccaggccgaa tgttaagggc caaaggctcc cttgccgggc
     6661 ccaattgatt gcctgttcaa ccgtcgtgat gacaagccct tcttcgaacc gtccttcaat
     6721 ccaaggctgc gtcatgtctc gttaccgtct cgggttccct aggctccgcc gcgctccaac
     6781 gtcaaataaa ccaatcattc actatacctc aagtcgacgc cgcctgtaca cgttcccagc
     6841 gattgtcact atccgagcaa atgttttccg cagcctgaaa ccgacattcg cttccaccct
     6901 cccgtcggca ttgcgtcaga tgaacctcaa tccccaacaa ctcactcaac caaagtcgct
     6961 cccactggca gatctgaccg tcaatctccg ccaagtccgg atatgggcag gcctggccaa
     7021 ccaggatagg taatcgagtt ccacgggacg cggttgcatc gccagggctt gccacactcg
     7081 gacgggacgt agcttggaga ttcgagccac tccccgtttc gcccgttgct tcaaccgaaa
     7141 aaggtatccg tttttgcgac aggaggcggc caacctcggc catacgctcc gaaacggtca
     7201 gtcccttgac ttgccgccag tacatgcggg ccatttcgtg ggcggcggca gtcagaacgt
     7261 gtgctcgtac cttggggtca tcaatcgatc gaatcgcccc ccacaaggcg attgctaggt
     7321 ccgtatagtt ggccccggcc gtttgtcgtc ccttcccagt gatcccgtag acataatgcg
     7381 gccgaccacg tcctgcgcgt gaggcctggc gctcgatcag cccttcggca tgcaaacgat
     7441 tgagtcgttg gcggatcgcg gtgggggtga cggaaaaccg ttccgcgagc tccgccaccg
     7501 tcatagggcc tgcgtttcgc aaacagtcca gtatatccaa gtcgctttgc acttggggaa
     7561 cacccatcgg cccggcgtcc ttttccttca cctaaatcgt gtgaggcacc aaccaaatcg
     7621 tcggtggtat ggcaccacct atcgtggtac ttggaccgcc ttttgcgtgg tgacctgagg
     7681 acgtgcctcg ttaacatcgt aacggattga cgcaatttcg acaagtctaa ttgtatcaat
     7741 aagcgcggtt ctaacttcaa aatccgtcaa aaaaccgact tttcggctcc tcgttgcatg
     7801 tggagcccag accggacacc ttgaacggca aatttggttg ccataagcga attcaccatg
     7861 acattgtggg caacgacgga tatcgctata attccagcga agttcggcac tttttgccga
     7921 tttcttacgg attctacacg ttacgcaaac aaccggcgtt ttctgatagc cgtcacagaa
     7981 tcgtccggaa cgaaattcgc ttaattacca gcccaagata aatgacatgg cacagacgtt
     8041 tcgggtcgga cttggcactg acctccaccg actggtggag cctggcccat tgattctggg
     8101 gaatattgcc attcctttcg agaagcatgc tctgggccac agtgatgccg acgccttaat
     8161 tcacgcaatg attgacgcaa tcctaggggc atccggtggg ggggatatcg gccagttgtt
     8221 tcccgacacg gacgaggcca atcgggggcg ggattcgggc gaaatgttga gcatcgtctg
     8281 ggggcgcgtc cgagcacaag ggtgggccct gggtaacgtg gactgtgtcg ttcatttgca
     8341 gcgtcccaag ctcgcaccgt acatcccccg gatggtggag cgcctcagcg aattgatggc
     8401 ggtcccgcct gagaccatca gtattaaggc caagaccggg gagggcattg gactggtagg
     8461 acgagaagag gccgtcgctg ccgaatgcgt cgtcctactt gtgcgcgaga cggattcatc
     8521 atgccagtaa caggaggagc atggttctga taaagggcgc gtcagtccta caatgggtgt
     8581 cggatcaagt cgtgaaagat ggaacgccat tcaacgcacg aagggtctat gattcgggtc
     8641 tataacacac tcacacggaa gaaggaagtt tttgaaccgg tccagcctgg caaagtcggc
     8701 atttatctct gtggtccgac cgtctataaa ccaagccaca ttggccacat ggttggaccg
     8761 attatcttcg acgcaatcaa acgctatctt acatacaacg ggtataaagt cacttgggta
     8821 gttaacatca ccgatgtcga tgacaaactc atcgcagaaa gccaacggcg tgggattccc
     8881 atgtcagaat tggccgagga gatgatcgcc gactatatgg ccaacctcca ggccttgggg
     8941 attgacggca tcgaccactt tccgcgggcc accgaaaaca ttgacgcaat cataacgttc
     9001 acggaagatc tcattcgaaa gggatacgcc tatccggctg ggggagatgt ctatttcgac
     9061 gtctctaagg tcccggatta cggaaaactg agccggcgaa aattggagtc catgcttcct
     9121 gagggcggtg aaggggtgga gcgaaagcga tccccctacg attttgccct ttggaagggg
     9181 gcaaagccca acgagccttt ttggaacagt ccctggggac cgggacgtcc tggctggcat
     9241 atcgagtgct ctgtcatgag cagtcgcctt ttgggcgaga cgttcgatat tcacgggggc
     9301 ggattggacc tcatctttcc tcaccacgag aacgagatcg ctcaaagcga agcacgccat
     9361 ggcaagccca tggtgcgtta ctggatgcac aatggcttga tgcaggcggc ggacgagatt
     9421 ggaaaggtgg gtggccgcaa tacgcgggtc gcagaggacg acttggatgc ccagcaggcc
     9481 ggaaagatta gcaaatccaa gggtgccagt cccttccgcg atcttttgaa ggagtatcgg
     9541 ccggaaacca tccggctttt cattttgtcc acccattatc gccgtccgat tgaattcagc
     9601 gagcggcggc tccaggacac ggaaaccgcg atcgagacct tctatcgctt ctttgagcga
     9661 tttgagcgga tcacacgtca gtcgttctat tccttacccg tccccaaaag ccgaaaggag
     9721 ggcgatcttt tggacgggaa cgacgcgttc atgcaggacg tcgccggcca cagagcccgc
     9781 ttcctggagt ttatggacga cgatttcaac acaggagggg ccattggcga attgttcgag
     9841 ctcgttcggc ggctcaaccg attcgtggat gaacacaagc tggaagaccc tcaacaccga
     9901 accccagaaa agacggcggc tctggttcgc ggcgctaccg tactacggga actgggtcat
     9961 gtcctagggc ttttccgtca accaccgccc a
//
LOCUS       DAOSUT010000148         9953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3728_length_9953_cov_9.453930, whole genome shotgun sequence.
ACCESSION   DAOSUT010000148 DAOSUT010000000
VERSION     DAOSUT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_9953_cov_9.453930"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLD05_12395"
     CDS             <1..193
                     /locus_tag="PLD05_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78287.1"
                     /translation="QLPHYLFTRLCPTVDGLLFRIPVLRPLLSAEFKSINLTENRLGV
                     KRVATFRETQSLNYNAYPA"
     gene            741..2615
                     /locus_tag="PLD05_12400"
     CDS             741..2615
                     /locus_tag="PLD05_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOP78288.1"
                     /translation="MVNRNLIRHVDLSQEEWERELQAACGGMEPEELALAASDIAVNQ
                     IVEGRILRVERDFVVVDVGSKSEGIIPLSEWSENEEPPQPGQTISVLVEELEDVYGLG
                     DDYRGMITLSKRKAEKIRAWMRIMETVKEGDVVTGVVTRKIKGGLLVDIGVNVFLPAS
                     QVDIRRPADIGEFIGQTVQCVVLKIDEARRNIVVSRRALIERERAEKKAKLLAELEVG
                     QLRKGIVKNIADFGVFVDLGGIDGLLHITDMSWGRINHPSELVSLDQEIEVVILNIDR
                     EREKIALGLKQKTPSPWEDIELRYPVGSRVKGTVVNVMNYGAFVKLEEGVEGLVHISE
                     MSWTRRINHPSEVVQVGDEVEVVVLGIDKEKQEISLGIKQTMTNPWDTVEERYPVGSV
                     VKGIVRNITNYGAFVELEEGVDGLLHVSDLSWTRKITHPSEVLQKGQEIECRVLSIDK
                     SRRRIALGLKQLTEDPWETYIPTKYAPGQIVKGKVTKITNFGVFVGLEDGLEGLLHIS
                     ELADHKVDNPEQVVKVGEEIEVKILRVDKEERKIGLSRKRLEWAEEEQEAQQAAPEAK
                     EEKKEKEKEKELKGGLGASISRLIQPFIGGQAQQTQQEAEAKEESSTDSQESEEGSTA
                     "
     gene            complement(2734..3750)
                     /gene="trpD"
                     /locus_tag="PLD05_12405"
     CDS             complement(2734..3750)
                     /gene="trpD"
                     /locus_tag="PLD05_12405"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOP78289.1"
                     /translation="MLEAAVAKAERGANLTMEECESAVEAIIRGQAPEEVVARFLIAL
                     HVKGETAAEIAGAARAMRRHMLTVESSHPIVMDIVGTGGDGSGTFNISTAAAIVIAAA
                     GVPVAKHGNRRITSRTGSADVLAELGINIEAPLPVVTACLEELGICFCFAPLMHPAMR
                     NVAEVRRKLGHPTVFNLLGPLTNPASVPYQVLGVGRTFLRPLLAEALQLLGTTRSAIV
                     YGCGELDEVSLLGETQATIVGPDGVEEVIWKPEDFGLEPISCEALRVSGPGESAARIK
                     EMLDGTPGPIMDTVLANSAVGLWLVGRVNSLSEGVALARETILSGKARDLLAKWAERS
                     HRST"
     gene            3868..4833
                     /locus_tag="PLD05_12410"
     CDS             3868..4833
                     /locus_tag="PLD05_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOP78290.1"
                     /translation="MPRKVIVDLDPGIDDALALCMLLFDPDWDVVAVTAVGGNVPPHV
                     ATRNAQALIAYLDPPRIPRIGAATAPERGLPVDGRFANSVDDLRDASLTVAELRTPHP
                     SEKLLTDEVRAAPHAVTVLALGPLTNIARAIQRDPEFPVLVHRLIIAGGAIQVPGNIT
                     PAAEFNIYCDPHAAKIVFASKAAKTLVPLDVTNRVNFSLSFLNELPPETNKIGALLRK
                     ILPAKFRAYRQHLGLETIHLHDSLTYLAACRPDLFRYEELAGDVETVGEITRGATIFD
                     RRPVPAWQENRLEVAVGIDAAAIRLELLKRLNAAAQAAGNVESPP"
     gene            4907..>5337
                     /locus_tag="PLD05_12415"
     CDS             4907..>5337
                     /locus_tag="PLD05_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78291.1"
                     /translation="MASLEQATATLGHRPRYNMVLLWAVCLVAAMGGFLFGYDWVVIG
                     GAKPFYEPYFAIPPKTFWQGWAQSSALVGCLMGAAISGALSDRLGRRRLLITAGFLFT
                     LSAIWTAIAWDYTSFNLARITGGIGIGLASNLSPMYIAEIAP"
     assembly_gap    5338..5437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5438..5962
                     /locus_tag="PLD05_12420"
     CDS             <5438..5962
                     /locus_tag="PLD05_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78292.1"
                     /translation="QEVFQAAGYAVSDVLVNIVFTGIVNLVFTFVAIFTVDRWGRRFL
                     MLFGAGGLALIFGLLGLGYYFHSTGWHMLVLVLAAIGCYAMSLAPITWVVISEIFPNR
                     IRGLAMSVAVLSLWAACTALTLTFPILNRNLGPHGTFWLYAGVCAVGFLTILATLPET
                     KGKTLEEIERELVR"
     gene            complement(5972..7000)
                     /locus_tag="PLD05_12425"
     CDS             complement(5972..7000)
                     /locus_tag="PLD05_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78293.1"
                     /translation="MTSITLTRVTKWSSFQHGWVTGILVCLLIASSALGRDIFVNNES
                     GDDSFTGGIAEAGQRVDGPVRTIARALALAQPGDRIVLAKTNTPYRESLTLAGKRLSK
                     DNLGDFILEGNGAILDGSAPVPPEAWQVDKDSWQRFKRAVFRFTPEGKSYQQLFLGDR
                     PAVRVYADRLSAVPPQLEPMQWALFQGSIYFCVERDRHPADYPLRYAKLPVGITLHHV
                     EGVTIRDLVIQGFQMDGINLANTARDVRLERVVLRGNGRTGLTVGGACRGTIIDSLIG
                     DNGFAQILTLPNSEIHVQKCQVLGNTAPAWVDEGGRFFLAGELRKGGLDDIDAEAGTP
                     QTSDPGQN"
     gene            7272..8960
                     /locus_tag="PLD05_12430"
     CDS             7272..8960
                     /locus_tag="PLD05_12430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOP78294.1"
                     /translation="MNELHEECGVAAVYHLPGRPVSPLCPGGPMSAPRLIPRLLLDIQ
                     NRGQLAAGITRYCEGDSQLLLTYKNVGTVAEVFSLAQRTRFDDLMTKFSGSAALGHVR
                     YATCGKDDPSYAQPFERHHIKKHKWFSFAFNGQLANYLELRERLLRDEDNHLIRDTDT
                     EVIMHVLCRELNPNPAPDLAQVCRALPREFDGAYSLVFLNALGELVVLRDPLGFKPLS
                     WAFDGSLFAAASESVALLNLGFPPEAIKPVKPGQAIIVSKEGIRFEQFGNAPHCAHCF
                     FEWIYFSNVASTLDGRSVYLARKALGEELARLETLPVDDGRTIVVPVPDTSKAAADAM
                     AFALGVPCLEGLIRNRYTGRTFIDSHMTREERANAKYTPLREVLEGKRVLLVEDSIVR
                     STTMRVLVHRMRALGRPAEIHIRVACPPIISPCFYGIDMSTMSELFAPRYITNGELTE
                     AIQARMAKDLGADSLRYLPVEAVARAIGIDPKDLCQACITGSYPTPWGQKLAEAARAR
                     SLRGETGRTYDAIDTTSERCSRPSPGCDRGLSPAGIASTEGDGPDGYGDTSGNR"
     gene            9240..9950
                     /gene="rpe"
                     /locus_tag="PLD05_12435"
     CDS             9240..9950
                     /gene="rpe"
                     /locus_tag="PLD05_12435"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOP78295.1"
                     /translation="MSVHSPLYTDWTLGHVMIGPSLLAADFRYLAREIERLEAAGARF
                     LHLDIMDGHFVPNLSFGIPIVQAVRRCTNLTLDVHLMISNPEAHLERFRDAGADYLTV
                     HIEVAPDPRPLFEQIHRLGACAGVSLNPPTPVETVLPYLNDCELVLVMSVMPGFGGQT
                     FQPEVLAKIRRLRQHSPKGLMISVDGGVNGDTIGHCAAAGATMFIVGTGLLGHNNYHQ
                     RYQELASMVKKSQEKVEL"
BASE COUNT         2133 a         2805 c         2766 g         2149 t
ORIGIN      
        1 gcaactgcca cactaccttt ttaccagact gtgcccaact gttgacgggc ttctctttcg
       61 gattcccgtt ctccggccac ttttaagtgc cgagttcaaa agcattaacc tcacggagaa
      121 ccgcctgggt gtcaagcgag tcgcaacttt ccgtgagacg caaagtctca attataacgc
      181 ctatccggca tagtgttgga ggcttcttac ccccaccgac gatctaggcg aagtattaca
      241 tgcacggttt catccaatca caacaatttc ccctgtcgta tttctcggca caagtcgcaa
      301 ttcctaccca gaagaatcca aatatgactc aatttggggg atataggcaa tctgcggaca
      361 acgctcattc gcggccgttc ccctggttat gccttacgcg atttctcccc tcaacacgac
      421 gtccgcgcga atccaaaacc tcgctcgggc gcaattgtcg cacggctctt cctctgttat
      481 actctgtggg tgttggtagg ttgcggtatc cctcgcaagg ggcgagggac aatctgccaa
      541 agtggtaggt gcggattgct ttttcgcaag atcgggtttc gttgcggcca acctctgaca
      601 atttcgcgga tggtggttgt cggagacccc tcactggtgc agggacaggg gagattgaca
      661 aagtctccgg ctgcatcgtc ccccttccaa cgttcagctt ccgcgctaac tctttttgac
      721 gttctaggtg ttctctcaac atggtcaatc gtaatctcat tcgtcacgtg gacctttctc
      781 aagaggagtg ggaacgagaa cttcaagccg cttgtggggg aatggaaccg gaagagcttg
      841 ccctggcggc gtcagacatc gcggtcaacc agatcgtgga aggccgcatc ttgagggtgg
      901 aacgcgattt tgtcgttgtc gatgtcgggt caaagagcga gggcatcatt cctctttcag
      961 agtggtcgga aaacgaagag ccgccgcaac cgggtcagac aatcagcgtc cttgtggaag
     1021 agctcgagga cgtctatggc ctcggtgacg attaccgggg catgattacc ctcagcaagc
     1081 gaaaggcaga aaagatccgc gcttggatgc ggatcatgga gacggtcaaa gagggtgacg
     1141 tagtcaccgg ggtggttacc cgcaagatca aaggcgggct tcttgtggat attggcgtca
     1201 acgtcttctt gccggcgagt caggtcgata ttcgaagacc ggccgacatc ggtgagttca
     1261 tcggtcagac cgtgcagtgc gttgtcctga aaattgacga agcacggcgg aatattgttg
     1321 ttagtcggcg ggctttgatc gagcgagagc gtgcggaaaa gaaggccaaa ctcctggccg
     1381 agctggaggt cggtcagttg cggaaaggca tcgtcaagaa catcgccgat tttggtgtgt
     1441 ttgtggatct gggcgggatt gacggcctgc tccacatcac ggatatgagt tggggccgga
     1501 tcaatcaccc ctccgaactg gtgtctctgg atcaagaaat cgaagtcgtc atcctgaaca
     1561 tcgatcgcga gcgcgagaag atcgccctcg gcctgaagca gaagaccccc agtccctggg
     1621 aagatatcga gcttcggtat cccgtgggta gccgtgtcaa gggtacggtg gtcaacgtca
     1681 tgaactatgg ggccttcgtc aagctcgaag aaggtgtcga aggtctcgtg catattagcg
     1741 aaatgtcgtg gacgcgccgc atcaatcacc ccagcgaagt cgtccaggtt ggagatgagg
     1801 tcgaggtcgt cgtcctcggg atcgacaagg aaaaacaaga gatttccctg ggcatcaagc
     1861 agacgatgac gaatccctgg gataccgttg aggaacggta ccccgtcggc tcagtggtca
     1921 aggggatcgt ccggaatatc acaaattacg gcgcctttgt ggagttggaa gagggtgttg
     1981 atgggctgct ccacgtcagc gatctgtcgt ggacccgcaa gattacccat ccgagcgaag
     2041 ttctccaaaa gggccaggaa atcgagtgcc gtgtgctgtc gatcgacaag tcgcggcggc
     2101 gaattgcctt gggcctcaag caattgaccg aggacccctg ggagacgtac atccccacca
     2161 aatacgcccc tggtcaaatc gtcaaaggca aggtcaccaa gatcaccaac tttggagtgt
     2221 ttgttggcct ggaggacggt ttggaagggt tgctccatat ctccgagttg gccgaccaca
     2281 aggtggacaa tcctgagcaa gtcgtaaagg tcggcgagga gatcgaggtc aagatcctcc
     2341 gcgtcgataa ggaagagcga aagatcggtc tgtcgcgcaa gcgtctcgag tgggccgagg
     2401 aagaacaaga ggctcaacaa gcggctcccg aagccaagga agagaagaag gaaaaagaga
     2461 aagagaagga actcaagggt ggcttggggg cttccatcag ccgtttgatc cagcccttca
     2521 tcggtggcca agcgcaacag acgcagcaag aggccgaggc gaaagaggaa tcctcaaccg
     2581 actctcagga atccgaggaa ggctcaactg cgtgagcatg gactatcgtc gtttggtgag
     2641 gacctagcca aaacggtttt tcccctgtaa ctgccagtgg tcaccctaaa ccgtagaggt
     2701 atcgcacgta cctctacggt tttttgttga ggatcacgtt gaccggtgtg accgctcggc
     2761 ccatttggcg agtagatcac gggccttgcc gctgaggatg gtctcccggg ctaaggccac
     2821 gccttcgctg agcgaattaa cccggccaac caaccacaaa cccacggcac tgttggccag
     2881 gacggtatcc atgatgggtc ccggcgtgcc gtcgagcatc tccttaattc gggcggcact
     2941 ttctccgggt ccacttaccc gcaacgcctc gcaagaaatc ggctccagtc cgaaatcttc
     3001 tggtttccaa ataacttctt ctacaccgtc tggtcccacg atggtggcct gcgtttctcc
     3061 cagaaggctc acctcatcca gttcgccaca cccatagacg atggcgctcc gcgtcgtccc
     3121 caatagttgc agggcctcag ccagaagggg acgcaaaaac gttcggccaa cacccagcac
     3181 ttggtaagga acactcgccg ggttcgtgag cggccccaag agattgaaca cagtaggatg
     3241 accaagtttc cgacgtactt ccgccacatt tctcatggcc ggatgcatca gcggagcaaa
     3301 acagaagcag atacccagct cttccaggca tgcagtcaca acgggcaatg gggcctcgat
     3361 attgatccct aactccgcca acacatccgc ggagcccgtc cgactcgtaa tccgccgatt
     3421 tccatgcttg gccaccggaa cgcccgctgc cgcaataaca atcgccgccg ccgtgctgat
     3481 gttgaacgtc cccgagccgt caccgccggt ccccacgata tccatgacga tgggatggct
     3541 actctcgacg gtgagcatat gacggcgcat cgcgcgcgca gcgccggcaa tctccgcggc
     3601 cgtctcgcct ttgacgtgaa gcgcgatcaa gaagcgcgcg accacctctt ccggagcctg
     3661 gcctcggatg atggcctcca ccgcgctttc gcactcttcc atcgtcaaat ttgccccacg
     3721 ttccgctttt gcaacggcag cttccagcat ggcattttcc ctttctcctg acgaccacat
     3781 gcccttgcga cgatgagcaa ggaaacctaa aatcgggcca tatcccactg tcaaatagga
     3841 attgacccca cctgacgagg gtaagcgatg ccacgaaaag tcatagttga tctcgatcct
     3901 ggaatcgacg acgcgctggc attatgcatg ctcctctttg atcctgattg ggacgtcgtg
     3961 gcggtcacag cagttggagg aaatgtcccc ccgcacgttg ccactcggaa cgctcaggca
     4021 cttatagcat acctggatcc cccgcgaatt ccaagaatcg gggcggccac cgcacccgag
     4081 aggggtctcc ctgtggatgg ccggttcgcc aattcggtag atgatcttcg agacgcgtct
     4141 cttactgtcg cggagctaag aaccccccat ccttcggaga aacttttgac cgacgaagtt
     4201 cgggcggccc cccatgcggt aaccgtgctc gccctcggtc ccctgaccaa catcgcccgt
     4261 gccatccaac gtgatcccga gtttccagtg ttggtccatc gactgattat tgccggcgga
     4321 gccattcaag tccccggaaa tattacgcct gcggcggaat tcaatatcta ctgcgatcct
     4381 cacgcggcca agatcgtttt cgcttccaag gctgctaaga cccttgtccc cctggacgtg
     4441 acgaatcgcg tcaacttttc cctttccttt ttgaacgaac tgccgccgga aacgaacaag
     4501 attggcgcct tgttgcgcaa gattttgccg gcaaagtttc gggcgtatcg gcaacacttg
     4561 gggctagaga ccatccacct ccatgactcg ctaacttatt tggcggcctg ccgtcccgac
     4621 ctcttccgtt acgaagaatt ggcgggggac gtggaaacag ttggcgaaat cacccgggga
     4681 gcgaccatct ttgaccgccg ccctgtccct gcctggcagg aaaaccggct tgaggtcgcc
     4741 gtgggcatcg atgctgctgc catccgcctg gaattgctca aacgccttaa cgcggcggcc
     4801 caggcagccg gcaatgtgga atcacctccg tgacgcatcg attcccccac tccgttggga
     4861 cagttgagag cagttttcac attccgtttg ggagggcacc gactgcatgg catctctgga
     4921 acaagcaaca gctacattgg gccaccggcc ccgttataac atggtcctcc tctgggccgt
     4981 atgtttagtt gccgccatgg ggggatttct cttcggctac gattgggtcg tcatcggcgg
     5041 ggccaaaccc ttttacgaac cttattttgc catcccaccg aagacctttt ggcaaggttg
     5101 ggcccagagc agtgctttgg tcggatgcct catgggagcg gccatttccg gggccctcag
     5161 tgaccgcttg gggcggcggc gtctcttgat tacggccgga ttcctcttca ccctttcggc
     5221 aatctggacg gccatcgctt gggactatac ctcgtttaat cttgctcgaa tcaccggtgg
     5281 gattgggatc ggcctggcct ccaatctttc gcccatgtac atcgcggaga ttgccccnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa gaggtcttcc aggctgccgg
     5461 ttacgccgtc agtgacgtcc ttgtgaacat tgttttcact gggattgtca acctggtgtt
     5521 tacgtttgtg gccattttca cagtggatcg ttggggccga cgctttctca tgctctttgg
     5581 ggccgggggc ctggctctta tctttggcct ccttgggctg ggttactatt tccacagtac
     5641 gggctggcac atgctggtgc tggtccttgc ggcgatcggc tgctacgcca tgtccttggc
     5701 cccgattacg tgggttgtca tctcggaaat cttccccaat cggattcgtg gactggccat
     5761 gtcggtggct gtcctgtcct tgtgggcagc ctgcacagcc ctgacgttaa cgttccctat
     5821 cctcaaccgc aatctgggtc cccacgggac gttttggctt tatgcgggcg tctgcgcggt
     5881 tggttttctc acaatactgg cgacattgcc agaaaccaag ggtaagacct tggaggagat
     5941 cgagcgcgaa cttgtaagat gaggccaacg atcaattttg gccgggatcc gaggtctgcg
     6001 gtgtgccagc ctccgcgtcg atgtcgtcaa gaccaccctt ccggagttcg cccgcaagga
     6061 aaaaccggcc accctcatca acccaggccg gggctgtatt tcccagaacc tgacatttct
     6121 ggacatggat ttcagaattg ggaagcgtca ggatttgtgc aaagccatta tcgccaataa
     6181 gggagtcgat gatcgtccct cggcaagcgc cacccaccgt gagacccgtt cgcccgttgc
     6241 cccgcaagac caccctttcc agacggacat cccgtgcggt attggccaaa ttgattccat
     6301 ccatttgaaa gccctggata accaggtcgc ggatcgtcac cccttccaca tgatgaagtg
     6361 tgatccccac aggcaacttc gcataacgca ggggatagtc ggcggggtgc cgatctcgct
     6421 ccacgcaaaa atagatgctc ccttggaaga gggcccactg catgggttcc aattgcggag
     6481 gtacggccga taaacgatcg gcatagacgc gcacagccgg gcgatcaccg aggaaaagct
     6541 gctgatagct ttttccttcg ggcgtgaatc gaaaaacagc ccgtttgaat cgctgccagg
     6601 aatccttgtc cacttgccag gcctcgggcg gcacgggagc cgagccgtcc aagatcgccc
     6661 cattaccttc caggataaaa tccccaaggt tgtccttgct caaacgtttc ccggccagag
     6721 tcagcgactc ccgatacggg gtgttggttt tcgcaagcac tattcgatcg cccggttgtg
     6781 ccaaggcaag ggcgcgggca atcgtgcgaa caggtccatc caccctctgt cccgcctcgg
     6841 caatcccccc ggtaaaagaa tcatcgcccg attcgttgtt gacaaaaatg tcacgtccca
     6901 aggcagagga ggcaatgagc aaacaaacga gaattcccgt cacccaaccg tgttggaagg
     6961 aagaccattt cgtaacccgc gtcaacgtta tagacgtcat atctctcgtt ccaatatggg
     7021 ttacacgtct tggaaatgtc gtcaccaaat tccaacggat tacaggaaag aatcttttgg
     7081 tccagtgtat catcaccaaa atcgaatcgc caccgaatcg ggagcggcca ggacagagcg
     7141 tggcccccac ccggctcgtt gtagcgaatg ccttcaaacc gtaaaataag agaggaagtg
     7201 ctacctatcc ccggggtcgg gcgggttgtc tgccgacaat gcccggaact ctctttgagg
     7261 cgggaacatc aatgaacgaa cttcacgagg agtgcggcgt tgccgcagtc tatcatctgc
     7321 cgggtcgtcc ggtgagtccc ctttgtcccg gcggcccaat gtcggcgccg cgactcatcc
     7381 cgcggttgtt gctcgacatt caaaaccgag gccaacttgc tgcaggaatc actcgctact
     7441 gtgaggggga ctcgcagctc cttttgacct ataagaacgt cgggacggtc gccgaggtct
     7501 tttccctcgc gcagcggacg cggttcgatg atttaatgac aaagttttcg gggtcggcag
     7561 ccctgggaca cgttcgctac gcaacctgtg ggaaggacga ccctagctac gcacaaccct
     7621 ttgaacggca ccacattaag aagcacaaat ggttcagttt cgccttcaac ggacaactgg
     7681 ctaactacct agagttgcgc gaacggctac tgcgggacga ggataaccat ctgatccgcg
     7741 acaccgacac ggaagttatt atgcacgtgc tgtgtcggga gcttaacccc aatcctgccc
     7801 ccgacctggc gcaggtgtgt cgcgcacttc cccgagagtt cgacggcgcg tacagtttgg
     7861 tcttcctcaa tgccctgggg gaacttgtgg tcctccgcga ccccctgggt ttcaagcctt
     7921 taagctgggc atttgacggc tcactgtttg cggcggccag cgaaagtgtc gcgcttttga
     7981 acctgggatt cccgccggag gccatcaaac ccgtcaaacc cggtcaagcc atcatcgtct
     8041 ccaaggaagg catccgcttt gagcagtttg ggaacgcccc ccattgtgcc cactgctttt
     8101 ttgagtggat ctatttttcc aatgtggcga gcactctgga tggccgaagt gtttacctcg
     8161 cccgcaaggc tctgggcgaa gaactggccc ggctcgagac actccccgtc gatgacggcc
     8221 ggacgatcgt cgtgcccgtc cccgatacca gcaaggcagc cgccgacgcc atggcatttg
     8281 ccttgggtgt cccttgcttg gagggcttga tccggaaccg gtacacggga cggacattta
     8341 tcgacagcca tatgacacgg gaagagcggg caaacgcaaa atacacgccg ctccgcgaag
     8401 ttctggaagg gaagcgggtc ctcttggtgg aggactcgat tgtgcgatcc acaacgatgc
     8461 gggttctcgt ccaccgcatg cgggcgttag gccgtccggc agaaatccat atcagggtgg
     8521 catgtccgcc gatcatctcg ccgtgtttct acgggatcga catgtccacc atgtccgagt
     8581 tgttcgcccc ccgttacatc acaaatggcg agttaacaga agcgatccaa gctcggatgg
     8641 cgaaggacct cggggccgac tcgctgcggt atctgcctgt ggaggccgtg gcccgggcga
     8701 tcggaatcga tcccaaggac ttgtgccagg cctgcattac gggttcgtac ccaacacctt
     8761 gggggcaaaa actagccgag gcagcccgcg caaggtcgct tcgcggagag accggccgga
     8821 cctatgacgc gatcgacacc accagcgaga ggtgttcaag gccatccccg ggttgtgacc
     8881 gcggtctgtc gcctgcggga attgcctcga cagaggggga tggaccagat ggctatgggg
     8941 atacgtccgg caacaggtga atctaaagcc ggggttttgc ccggatacaa ctttatgggg
     9001 ataagatcat cgacgaagcg ggtgaatgac aaacggactc gcgtcgcata aactagtggt
     9061 ttattggtaa atatcgcggg tcttgaacaa ataggctgag aataaggaaa tcgaaacgac
     9121 tccctttgac cataaatggt gagatcaggt aaactttcgc cttggtaatg aactgatctc
     9181 acactgggga aaaattgggg tcactcgtct tgtgagatga taaggcaatt ccttctggga
     9241 tgagtgtgca ttcgccacta tatacggact ggacgcttgg gcatgtcatg atcgggccgt
     9301 cactcctggc ggccgatttc cgttatttag cccgggaaat cgaacgctta gaggcggcgg
     9361 gggcacgctt tttgcacttg gacatcatgg acggccactt tgtgccaaac ctgagctttg
     9421 gtatcccaat cgtccaggcc gtccggcggt gcactaattt gacgcttgac gtgcatttga
     9481 tgatttcgaa tccggaggcc catcttgagc ggtttcgcga tgccggggcc gactacctaa
     9541 ccgttcacat cgaggtggct cccgatccgc gaccgctgtt cgaacaaatc caccgcttag
     9601 gtgcgtgtgc cggtgtatcg cttaatccgc cgaccccggt cgaaacggtg ttgccctact
     9661 tgaacgattg tgagcttgta ctggttatga gcgttatgcc gggttttggg ggacaaacgt
     9721 ttcaaccgga agttctggcc aaaattcgcc gacttcgcca acatagcccc aagggactta
     9781 tgatttcagt agatggcgga gtgaatggag atacgatcgg tcattgtgcc gcagcggggg
     9841 cgacgatgtt catcgtcggc acaggcttgc taggccacaa taactaccac cagcgatacc
     9901 aagagctcgc atcgatggtg aagaaatcgc aggaaaaggt tgaactatga cga
//
LOCUS       DAOSUT010000149         9887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3752_length_9887_cov_9.799532, whole genome shotgun sequence.
ACCESSION   DAOSUT010000149 DAOSUT010000000
VERSION     DAOSUT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3752_length_9887_cov_9.799532"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLD05_12440"
     CDS             complement(<1..450)
                     /locus_tag="PLD05_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78296.1"
                     /translation="MLISLVAGLCYAFTIYRSLDSKLKRAIEAHCSELFPSLSIRIRS
                     AQVIIGEGLQIRGLRVLDPHGDRHSGLLLDVDELFLAGPVDYQQLLAKRLQIKRVVIR
                     RPHLHLVNRQQDGWNIARLFPLPRGSGQCVPLEIENGLIEIVDMRKSP"
     gene            complement(587..1873)
                     /locus_tag="PLD05_12445"
     CDS             complement(587..1873)
                     /locus_tag="PLD05_12445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOP78297.1"
                     /translation="MSRPHEPKTNHQERMCLVVVFLAALAVRVVAAVAWEWHFGPRFV
                     FGDSESYWHLARCLAHCQPYRFGDFGTVFRTPGYPALLAPLFWFFDSPPTLAARIEGS
                     LLGAIAALVTAIWAGNLFGSRARWLAGLIMAFHPEMVLSSILILSEGGFLPVWLVHLL
                     VLSQALSRTHAAKTAWKEWFLAGCLVGLATLIRPAAILWLPFCATVTITFFTRQRLQL
                     VILWAVCLLAFGLTMSLWWVRNYRVTGRWILTTTQAGPTLYDGLHPGATGASDMSFVP
                     NKFRELQQSRPDARPIDLELEFDRRLSAEAIRWAWSHPRQVVSLAIVKFRRLWNPFPN
                     DPGFNVWPINLIVAGCLLGVLVLGLAGCLKFKSIGWPVAVCWLPAVYVTLLHVIFVAS
                     IRYRWPAIPGLIVLGAGFLAETIQGIRVRDVKMVDT"
     gene            2408..3883
                     /locus_tag="PLD05_12450"
     CDS             2408..3883
                     /locus_tag="PLD05_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78298.1"
                     /translation="MAAQLPDYVTSTKPVPKDKRTPWYASTAPTYAGIFLWFVFWHQV
                     PLLKELTPGSGIPLTAGGALSQGLGIALLALIVSALICHYLFYLVPGKLGVQTGLPLY
                     VVGTSTYGVLGGFIMPGFLMGVLQFGWLAVNAFFTTGLLCAPFGAGPGTLTFTVVGIA
                     FVILATLVGLKGIQYVGKIATYLPLLPLFILVVLTFASVGGLSKFDHKKLVGAPAAAS
                     ENAPAGGEQKAEATSAASAKQMPDLSVWGVFMAICAYVVGFFATAGAAGVDFGMGNRD
                     ENDVKWGGLVGIAGAIILTGGLALIIAAGTVGAGLAGQPAVLQPTKLMENLAPVLGEK
                     APAVFWYLLAIAAFPPACFSSFIAANSFKTTLPKVNPFVSCGIGALASIALVLSGYAG
                     NAANVFTVIGASFGPVCGAMAADYILAGNRWAGPRAGFNPAGWVSWVAGFIVGAVDLL
                     LGTSYVPCPPVAAFIVGFVLYAILALAGLESELLPMPKEGE"
     gene            4141..4866
                     /locus_tag="PLD05_12455"
     CDS             4141..4866
                     /locus_tag="PLD05_12455"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOP78299.1"
                     /translation="MDGKTSHPRLVLPRRWVRELDRRAVEEYHIPSIILMENAGRGAA
                     DVLERLGINGPVVIVCGRGNNAGDGLVIARHLDLRGYKVKVVMLAPGESFTGDPFVNY
                     QIVRCAQLPMVILGEGCSEHEVLAHLNGADWIVDALLGTGAQGDPRPPFDVVIHAINR
                     AHDQGKKVLAVDLPSGLDCDTGQPSRATIRADHTVTFAAVKPGLIVDAARPFVGQLHV
                     ADIGTPRRLIDEIAAEYERESNS"
     gene            4894..5874
                     /locus_tag="PLD05_12460"
     CDS             4894..5874
                     /locus_tag="PLD05_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP78300.1"
                     /translation="MSSQPHHPPNGFGSPPLAVVPATGEGIRRGLWLLCKDLPEADRD
                     GHLRRLREHFRRSPPGTTGILQAQRGQEIVGAILWTVYPGRTAVISAPGLLENEPLTT
                     ARALFRGVVDNLANTQTRLIFANFSQDQGHFREPVEEAGFCYLARLLYLFSPVADLPN
                     SSEPPKCDLQFIPWPQVEHGDFVQIVEATYVDTLDCPKLNGLRETEDVLAGYRAAGVH
                     VPDLWFLIRFEQEPVGCLILTEQPDFDQLELVYMGLIPKYRGRGWGKDVVRFAQWMAR
                     HRKRAGLLLAVDTQNTPAMRVYRSLGMQPLEERDVYLFNLTPRDGGTYPG"
     gene            6370..8157
                     /locus_tag="PLD05_12465"
     CDS             6370..8157
                     /locus_tag="PLD05_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOP78301.1"
                     /translation="MAKDDKDIMSALPRAIADKVGNERYSLWFGPRTRFAWNGTELSV
                     YAPNPFYREWIQTVFAGAIEEACQSVIGYVPTISFYCASEGGLMEVHDAAACEEPIAV
                     ESRTREPRKPARTRSTVRKPAPTPQISAEELPLFQFASTTEDDESVPETSHSPVNGVA
                     SEVIPTRGIAENSEGESRPSFHASREQAILKVNSGAMGVGVLPREASSSEDCGNVPAL
                     EESGPRADVVGQGATSPIPGVARNAPPTRGHQPRAALSLSSFIVGDCNRLAFAAADAV
                     VRALGSYSPLVFHGPSGVGKTHLLEGILHAVKQRYPRIAAIYLSAEQFTSAYVEALHK
                     GGIPIFRQKHRGLQLFLLDDLHFLVGKRQTQTELLYTIDSLLKNGCQIVCAADRPPCE
                     IRELGPELVSRLEGGVVCRIDPPDYEVRIQIVARLVSESGIALSPEVQAWIAERFAKN
                     VRQLIGAARRLEAAGKALKRPLDVRLAEEILADMLGDAQSQIRFPDIEREVCLAFGLP
                     EGALQTNRKDREITYPRMLAMWLARKYTRANLAEIGRYFGKRSHATVISAQKKVETWM
                     REGHRLPSVKGERAVSEMLKELERRLRAS"
     gene            complement(8292..9626)
                     /locus_tag="PLD05_12470"
     CDS             complement(8292..9626)
                     /locus_tag="PLD05_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78302.1"
                     /translation="MSTHSSRQGREMKKGTSRRDFLKTTGVAGAGALTGLSLSRFVHA
                     SGQDPILKIALIGCGGRGTGAAANALEADPRTVLVAMADAFEDRLNSSLAALKKNHGD
                     RVQVTPETSFVGLDGYKKAIACDVDVVLLCEPPHFRPMHLRAAVEAGKHVFAEKPVAV
                     DAPGYRSVLESTEIARQKNLCIVSGLCWRYHKPTIETVKRIQDGAVGDIIVIQETYNT
                     GHIGGRDRDPNLTEMQFQLRNWYCFTWLSGDHNVEQHIHSLDKALWVMHDEPPAVAWG
                     LGGRQVRTEMPRYGNIYDHHAVCYEYANGVRVYSYCRQHANCWNDTTDHVFGTKGRAV
                     ILNKWQIEGAVNWKYEGESTNMYVAEHQALFSAIRKGEPINNGVYMARSTMMAIMGRM
                     ATYTGQRITWEEATNSQEALVPSAYTWDATPPIVPDEQGRYPVAIPGITKFV"
BASE COUNT         2171 a         2588 c         2874 g         2254 t
ORIGIN      
        1 gggactcttc cgcatatcca cgatctcgat cagcccattt tcgatttcga gcggaacgca
       61 ttggccggaa ccacgcggca gcggaaaaag acgtgcaata ttccagccat cttgctgccg
      121 attcacgaga tggaggtggg gccgacgaat gacaacgcgc ttaatctgga gcctcttggc
      181 caagagttgc tggtagtcca ctggaccggc caggaatagc tcatcaacat caagaaggag
      241 acccgaatgg cgatcaccat gaggatcgag gacacgtaaa ccgcgaattt gaagtccttc
      301 gccgataatg acttgcgcgg atcggatgcg aatactcaaa ctggggaata gttcagagca
      361 atgggcttca atagcccgct tcagtttcga atcaaggctg cggtaaatgg taaatgcgta
      421 acaaagaccc gctacaagtg agatcaacat tccccacttg cagaattgcc aaaccggtct
      481 tgcgggtctt cgccggtcct tcatcctaga cgaccatccg tggcattact gtgggtactc
      541 cataaccttg caggacattc aaaacaaaac cacgttgcga accagcctaa gtgtccacca
      601 tttttacatc gcgaactcgt atcccctgga ttgtttcggc caaaaaaccg gcccccagca
      661 caatcagtcc tggaatggcg ggccatcggt aacggatgga cgccacaaag atcacgtgaa
      721 gcagtgtgac atataccgcc ggtaaccaac agacggccac aggccaaccg atgctcttga
      781 attttaggca accagccagt cccaagacca aaactccgag caaacaacct gcgacaatca
      841 ggttaattgg ccagacgtta aatcccggat cgttaggaaa cggattccac agtcgccgaa
      901 acttgacaat agccaagctc accacctgcc gaggatgtga ccaagcccag cggatggcct
      961 cggcagagag gcgtctgtca aattcaagct caaggtcgat cgggcgagcg tccggcctcg
     1021 actgctgcaa ttcgcggaat ttgttcggca caaagctcat atcactggcc ccggtagcac
     1081 cgggatgcag accatcgtaa agggttgggc ctgcctgggt tgtggtgaga atccatcgtc
     1141 cggttacccg gtagttccga acccaccata aagacatggt caatccaaag gccaaaagac
     1201 agaccgccca aagtatgacc agttgcaaac gctgccttgt gaagaaagtt atcgttacgg
     1261 tggcgcaaaa cggaagccac aagatagctg ccggacgaat gagtgtggct aagcctacga
     1321 ggcagcctgc aaggaaccac tctttccatg ccgtcttggc tgcgtgggtt cgggaaagtg
     1381 cctgagaaag taccaataag tgaacaagcc agacaggtag gaatcctcct tcactgagga
     1441 tgagaatact cgacaggacc atttcaggat ggaatgccat gatcaagccg gctagccatc
     1501 tggcacgact gccaaagagg ttgcccgccc agatggccgt gacaagggcc gcgatagccc
     1561 ccagtaagct tccctcaatg cgagctgcca gggtcggcgg cgaatcaaaa aaccaaaaca
     1621 ggggtgctaa aagggcaggg tagccaggcg tccgaaaaac agtgccaaaa tctccaaacc
     1681 gatatggctg gcaatgagct aagcatcggg cgaggtgcca gtagctctcg ctgtcgccaa
     1741 atacaaagcg aggaccaaaa tgccattccc aggccactgc ggcgacgacc cgcacggcca
     1801 gggcagccaa aaacaccacg accaggcaca ttcgttcctg gtgattcgtc ttcggctcat
     1861 gcggccggga catgctcaaa atgaaacgct tcctttctgg ggtttagcct aaaactccga
     1921 cgacggcgaa aaagcggcgg ttactgtcag ccttgcaaca gacgtcttaa cgtcgcatca
     1981 agtcatacta gtgaggcttc agaatgggaa tggtacacgt attcggaagg ttacgtcaat
     2041 gcgaaaatgg gaaaacgaaa gtggtgggag aatatgagca acctgtgatg gtttggttct
     2101 gcaggaaagg tgtgggcagc cggtttggtg gaaacgagac gagaaaataa acagtcacaa
     2161 atgacgcact tctccttcct aatccaacgc ctgcgccgca tgctcaggtc gtgcgctgcg
     2221 acatgtgaca gaaatcagcc ttttccgaag taattcctca caaattggtt atggacaatg
     2281 tcaacagttc ggttatactc aatgccaatt gggacggtga tgatgctact gcttgcagtg
     2341 atgtgaggat gggtgtttct ttggtctcac gagaggtcct gttttgacaa gcaaaggagg
     2401 ttactcaatg gccgcgcaac ttccggatta tgtcaccagc actaagccgg tgccaaaaga
     2461 caaacgcacg ccgtggtacg ccagcacagc gcccacatac gcggggattt tcctttggtt
     2521 cgtgttttgg caccaagtcc cattgttgaa ggagctgact cctggaagtg gtatcccttt
     2581 gacggcagga ggggccctct cacagggatt ggggattgcg ctcctggcgt tgatcgtgtc
     2641 tgccttgatc tgtcactatc tgttttacct ggtgcccggc aaattggggg ttcaaactgg
     2701 gctaccgctc tacgtggtgg ggacatctac ctatggcgtc ctgggcggtt tcattatgcc
     2761 gggtttcctc atgggagttc tccaatttgg ctggttggca gtgaacgcct ttttcacaac
     2821 tggcttactt tgtgctccct ttggcgccgg cccgggtacg ctcacattta ccgtagtggg
     2881 gatagccttc gtgattttag cgacattggt tggcctaaaa ggcattcagt acgtgggtaa
     2941 gatagcgacc tatctcccat tgctgccgtt gtttattttg gtggtcttga cattcgcctc
     3001 cgtgggcggg ttgtccaagt tcgaccacaa gaagctggtt ggggccccag cggcggcaag
     3061 cgaaaatgca ccggcaggtg gggagcaaaa ggcagaggcg acctccgcag cgtcggccaa
     3121 gcagatgccg gacctttcgg tgtggggcgt gttcatggca atttgcgctt atgtggtagg
     3181 cttctttgcg acggctggag cggctggggt cgatttcggc atgggcaacc gcgatgagaa
     3241 tgacgtcaaa tggggtggtt tggtcggaat tgccggtgcc atcatcctca ccggcggtct
     3301 cgcgctgatc atcgcggcgg ggaccgtcgg ggctggcctg gccggtcaac ctgccgtgct
     3361 tcagccgact aaattgatgg agaaccttgc cccggtcttg ggagagaagg cgccggcggt
     3421 tttctggtat ctcttggcca tcgcggcttt tcccccggct tgtttctcca gtttcattgc
     3481 ggctaacagc tttaagacca cattgccgaa ggttaatcca ttcgtgtcat gcggcatcgg
     3541 cgcgctcgcc tcgattgcct tggtgctttc aggatacgca ggaaatgccg ccaacgtgtt
     3601 caccgtgatc ggggcctcct ttgggcccgt atgcggggcc atggcggcag actacatcct
     3661 ggccggtaat cgctgggctg ggccgcgagc gggcttcaat cccgccggtt gggtctcctg
     3721 ggtagcgggc ttcattgtag gtgccgtgga cctgttgttg ggcacgtcct atgtgccgtg
     3781 tccgccggtg gcggccttta ttgtcggttt tgtgctctac gccattcttg ctctggcagg
     3841 cctggagagc gaattgctcc cgatgccaaa agagggtgaa tgaaacgttt gaaagtggtc
     3901 ttttgagacc aacatactgg acggaatgat gtcggggggc cttcccaaaa aggccccttt
     3961 tcatttgatc ttttttgtca cacactgggt gtagattggt aggatagaaa atcgtcggaa
     4021 ttccattggc acgatgtttc cactacggga acgagcgttg cgtgagataa agagaattat
     4081 cgcgcaggcg atcggcaagc tgagatcatt tggaaagtga ttttgggtgt gaatgcggcg
     4141 atggatggta agacctcgca tcctaggctt gtacttccgc gacgttgggt ccgggaattg
     4201 gaccgacgtg cggttgaaga atatcacatt cccagcatta ttctgatgga aaatgccgga
     4261 cgtggtgcgg ccgatgtgtt ggagcggttg gggatcaacg gccccgtcgt gatcgtttgt
     4321 ggaagaggga acaacgctgg cgatgggttg gtcattgccc gccacctcga cctgcgcggt
     4381 tacaaagtca aagtcgtgat gcttgccccg ggtgagtcat tcaccggcga cccattcgtc
     4441 aattatcaaa ttgtccggtg tgcccagttg ccaatggtca tcttgggaga gggctgctcg
     4501 gaacatgagg ttttggctca cctgaacggt gccgattgga ttgtcgatgc gcttttggga
     4561 acgggcgcgc aaggagaccc gagaccgccc tttgatgttg tcatccatgc tatcaaccgt
     4621 gcgcatgatc aagggaaaaa ggtcctggcg gttgatttac cgagtgggtt agactgcgat
     4681 acggggcaac cgtctcgggc aacgattcga gcagatcata ccgtgacatt cgcagcagta
     4741 aaacccggtt tgatcgtgga tgcagcgaga ccgtttgttg gtcagctcca tgttgccgat
     4801 attggaacac cgcgccgatt gatcgacgaa attgcggctg aatacgaacg cgaatcgaac
     4861 agttaacact tggttgggta gggccagaag agtatgtcgt cgcaaccgca tcatccccca
     4921 aatggcttcg gttctccacc ccttgcagtt gttcctgcta caggagaggg aattcgacga
     4981 ggactgtggt tgctgtgcaa agacctgccc gaggcggatc gggatgggca tttgcggcga
     5041 ttgcgggaac actttcggcg gtcgcctcct ggcacgacgg ggattcttca ggcacaaagg
     5101 gggcaagaga tcgttggggc tatcttgtgg acagtttatc cggggcgaac tgcggtcatt
     5161 tccgcgccgg gattgcttga gaatgaacca ctgaccacag cccgggcgct gtttcgggga
     5221 gttgttgata acctggccaa cacgcaaacg cgattgattt ttgccaattt ttcccaagat
     5281 cagggacact ttcgtgagcc ggttgaggag gcaggctttt gctatttagc ccggctgctt
     5341 tatcttttct cccctgtcgc ggatctccca aatagttcag aaccgccaaa atgtgatctc
     5401 cagtttattc cttggccgca ggtcgagcat ggagactttg ttcagattgt cgaagcgaca
     5461 tacgtcgata cgctggattg tcccaaactt aacggacttc gcgagacgga agatgttctt
     5521 gccgggtatc gcgcggccgg tgtccacgtt ccggatttgt ggttcttgat aaggtttgag
     5581 caggagccgg tgggttgtct tatcctgaca gagcagcctg attttgacca attagaattg
     5641 gtctatatgg gtttgattcc gaagtatcgc ggccgtggtt ggggaaagga tgtcgttcgc
     5701 ttcgcccaat ggatggcgcg gcatcgcaag cgggctgggt tactccttgc ggtggataca
     5761 caaaacactc ccgccatgcg ggtttatagg tcccttggaa tgcagccact cgaagagcga
     5821 gatgtctatc ttttcaacct cactccgcgc gacggaggga catatcccgg ctgacgggtg
     5881 ttgccacatg gacacacctt cctatcggga gagtcgccac ttctcgagtg tcactggccg
     5941 ttggtgttaa gcgagttcta ttttccgtgc tcgtcgcaag tctattctgg caaataggtt
     6001 acgatagcgc aattgataag gaagcagtcg atttacaaac agtctcaaca ttttttcccc
     6061 aatccacggt tgagcaaaga agccttctgt gatcgcgccg ttgggaaaat cttcgcactt
     6121 tagggaagca atcggccgct ggaatttagc cacagaaaat tttagccctt agtcctcttg
     6181 tggggctttg acagcagtgc tggcaccggt agaatcacgc tgatcgggtg acacatggac
     6241 aggctaggaa tggattcaac cattcttcgg gcgcaactta tctgattgcg cggcctgtgt
     6301 aacgttggtc atgtttcagc aaagcgctga actattgcgc tgggattgcg cgccgggagc
     6361 cgtgaaacag tggccaagga tgataaggac atcatgtcgg ccttgcccag agcaatcgcc
     6421 gacaaggtcg gaaacgagcg atactccttg tggttcggtc cgcgcacgcg gttcgcttgg
     6481 aatggaaccg aattatcggt ctatgccccg aatcccttct atcgggagtg gattcaaacc
     6541 gtgttcgctg gtgcgatcga agaagcctgc caatccgtga tcggctatgt ccccacgata
     6601 tcgttctact gtgcatctga gggcgggctg atggaggtgc acgacgcggc ggcctgcgag
     6661 gagccgattg ctgttgagtc gcgcactcgc gagcctcgca agccagccag aacacgcagc
     6721 accgttcgca aaccagcccc gaccccacaa atctccgcgg aagaactacc gttattccaa
     6781 tttgcttcca ccacggaaga tgacgagtcc gttcctgaga cgagccattc tcctgtcaac
     6841 ggtgtcgcct cagaggtaat accaacgaga ggtattgcgg aaaactcaga gggcgaaagt
     6901 cggcccagtt ttcatgccag ccgtgaacag gccatcttga aggtgaactc gggggcaatg
     6961 ggcgtaggag ttctgccgcg ggaggcttct tcctctgagg actgtgggaa tgttccggct
     7021 ttggaggaat ccgggccaag ggcggatgtc gtaggtcaag gggctacctc gccaattccc
     7081 ggtgtggcca ggaatgcgcc tccgacaagg gggcaccagc cgcgagctgc tttgtcccta
     7141 agttcgttca tcgtgggaga ctgcaatcgg ttggcgtttg ccgcggccga tgctgtcgtc
     7201 cgtgcgttgg gatcctattc accgcttgtg tttcatggtc ccagcggcgt gggaaagacc
     7261 catttgctcg aggggatttt gcatgccgtc aaacagcgct atccgcggat tgcggcaatc
     7321 tacctttcgg ccgagcagtt cacaagtgct tacgtggaag cgctacacaa aggtggaatt
     7381 cccatttttc ggcaaaagca tcgagggctg caactctttt tattggacga cctgcacttt
     7441 ttggttggca aacggcagac gcaaacggag ttactctaca caattgacag tctcctcaaa
     7501 aatgggtgtc aaattgtttg tgcggccgat cgtcccccat gtgagattcg agagttgggg
     7561 ccggagctcg tgagtcggct ggaaggtggt gttgtctgcc ggattgatcc acccgattac
     7621 gaggtccgca tccaaattgt cgccaggctc gttagtgaaa gcggtatcgc gctttccccg
     7681 gaggtccaag catggattgc cgagcggttt gccaagaatg tacgccaact tatcggtgcg
     7741 gcccggcgcc tggaggctgc agggaaggcc ctcaagcgtc cgctcgacgt gcgattggct
     7801 gaggaaatct tagcagatat gctcggcgat gcccaatctc agattcgctt tcctgatatc
     7861 gaacgggagg tttgtttggc gtttggactt cccgaggggg ctctccagac aaaccgcaaa
     7921 gatcgcgaaa tcacctatcc tcgcatgctt gccatgtggc ttgcccgcaa atatacacgc
     7981 gcaaatctag cggaaatcgg acggtatttc ggcaaacgaa gtcacgccac cgtgatttct
     8041 gcccagaaga aggtggaaac ctggatgcgg gagggccatc gccttccctc tgtgaaaggt
     8101 gaacgggccg tgagcgaaat gctcaaggag cttgagcggc gtctgcgggc cagctaggat
     8161 ctttgggagc cttggtgagt cactgatccc ggatcagcag ccacaaaaaa ccggcaaaga
     8221 ccgttcgcgt atagtctttg ccggtggcta tgcgccgaaa ggttcacaaa gcggcgtgga
     8281 gctactggct gtcagacgaa cttcgtgata cccggaatgg ccaccggata gcgtccttgt
     8341 tcatcgggga caataggagg tgtagcgtcc catgtgtaag cggacggcac gagtgcctcc
     8401 tgggagttgg tggcctcttc ccaggtgatc cgctgtcccg tgtaggttgc catgcggccc
     8461 atgatggcca tcatcgtact gcgggccata tagacgccgt tgttaatggg ctcgcctttt
     8521 cgaatcgccg agaataacgc ctgatgttcg gcaacgtaca tgtttgtgct ttcgccttcg
     8581 tatttccagt tcacggcgcc ttcgatttgc cacttgttga ggatcacagc gcggcctttg
     8641 gtgccgaaga cgtggtccgt ggtgtcgttc cagcagttag catgttgacg acagtaagaa
     8701 tacacgcgga cgccattggc gtactcataa cacacggcgt ggtgatcgta gatgttgcca
     8761 tagcgaggca tttccgtccg gacctgtcgt ccacccaatc cccaagctac ggccggcggc
     8821 tcgtcgtgca tcacccatag ggccttatcg agactgtgga tgtgttgctc gacgttatga
     8881 tcacctgaca accatgtgaa gcagtaccaa ttgcggagct ggaattgcat ctccgtcaaa
     8941 ttgggatcac ggtcgcgccc gccgatgtgt ccggtgttat aagtttcctg aatgacgata
     9001 atgtcaccca cggcgccatc ttggatacgt tttacagttt cgatggtggg cttgtggtac
     9061 cgccagcaca aaccggagac gatgcaaagg tttttctgtc gggcaatctc tgtgctttcc
     9121 aggacggagc ggtaacccgg ggcatccacg gccacaggtt tctccgcgaa gacgtgtttt
     9181 cccgcttcca ctgctgcccg aaggtgcatc ggtcggaagt gtggcggctc gcacaacagg
     9241 acgacatcga cgtcgcaagc gatggctttc ttgtaaccat caagcccaac aaacgacgtc
     9301 tcaggggtga cctggacccg atcaccgtga ttctttttca gtgccgcgag gctgctattg
     9361 aggcggtctt caaaggcgtc ggccatggcg accaggaccg tgcggggatc ggcttccagg
     9421 gcgttggcag ccgcacccgt tcctcgaccg ccgcaaccaa tgagggcaat cttgagtatc
     9481 gggtcttgcc ccgacgcatg gacaaatcgg ctcaaggaga ggcctgtcaa ggcaccggcg
     9541 ccggcaacac ctgtggtttt tagaaagtca cgccgtgagg tccctttttt catttcacgg
     9601 ccttggcggg aggaatgcgt actcatcgct tcgtcctttc ctggcagggg caacactgaa
     9661 ggttttagat agcgaaaaca acctccaaca actcgcgagc atgattgtcg tggctgtagt
     9721 gccaccgacg accgatccca tcatactccc cgaccggtgc cgcgccaaca tccggcttag
     9781 ggcctatccc aaaattccct ctccctctgg gagagggtca gggtgagggc aagcgatcgt
     9841 cggagccgat cgagaaaacc agcggggatt cgagccaccc tcacccc
//
LOCUS       DAOSUT010000150         9715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3824_length_9715_cov_10.348344, whole genome shotgun sequence.
ACCESSION   DAOSUT010000150 DAOSUT010000000
VERSION     DAOSUT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_9715_cov_10.348344"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLD05_12475"
     CDS             complement(<1..452)
                     /locus_tag="PLD05_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78303.1"
                     /translation="MAFDGRGLLFTFLATMYFLGPTWRHHERCAQAEDTVAVYEGLKV
                     IAIGSTFTPGPRTILAQPLWDRGGNTSVRYTATEHLVWKDDGYFQRNRDLGQVFTAPR
                     DFVLSAIVLRTGPADGAVLHNAPGAVVFVEFFEVTGTPQIDNNGTGPGT"
     gene            complement(882..1970)
                     /locus_tag="PLD05_12480"
     CDS             complement(882..1970)
                     /locus_tag="PLD05_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78304.1"
                     /translation="MKVRKHTPNMRSRFGFTLIELLVVIAIIGILIALLLPAIQSARE
                     AARRSQCSNNLKQIGIALHNFHDAYKKFPTGGEGTNFKASPPETTFDMKDLHSVFTYI
                     LPFVEQKDIYDQINLGKTYRDTTAGIEHASAFRRELAVYTCPSNPFLEYKDPAGYGRL
                     DYYATVYTDIDPSTGHRNLATRVNGALAVPATSVAAIADGTSNTIMIIEDVGRCAPGS
                     GVQFASYSKYHDPTLDLQGQLAADDQAVTDNGNPHSGRPPYRAVWRWADQDAAGSGVS
                     GPSDACWDGRVINNNKSPYGGKAPNCTWDKNNIGLNDEPFSFHPGGCHALFSDGSVRF
                     LSEQIKPQVMRLLVSCAEGVPLPEDFGF"
     gene            complement(2589..4721)
                     /locus_tag="PLD05_12485"
     CDS             complement(2589..4721)
                     /locus_tag="PLD05_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOP78305.1"
                     /translation="MFRCHLHSKALLYVSILFLGGVATTGLIFAQPRPEWKPLGPLPG
                     DAMLADYFQVEVGKLQAACLADIKTLEDWQARCEEYRRQLREMLGLDPWPKKTDLKAT
                     ITGMLERDDFRVEKLHFQSMPGLYVTANLYLPKNITKPVPAVLYLCGHAQVKIDNVSY
                     GNKAHYHFHAVWFARHGYACLVLDSLQLGEIEGIHHGTYRYGMWWWNNRGYTPAGVEA
                     WNCVRALDYLQSRPEIDGERIGVTGRSGGGAYSWWIAAIDPRIKAAVPVAGITDLQNH
                     VIDGCVEGHCDCMYIVNTYRWDYPMVAALVAPRPLLIDNGDHDPIFPEDGVRRVYEQA
                     RKIYQLYGAEDKIAIFITTAGHDDIPTIQEAAFRWLDRHLMGKERDTYDPVEKVLTPQ
                     ELKVFETLPGDQLNTTIHEHFVPAAPVVFPQNQDEWKRMQTEWLKGIREKCFRGWPGD
                     LPPATLRDAVSVVRDGVRLTRAKLDVQDKITLPVYRLELAAGSVRRAIVEVLDQTDWQ
                     EWLKGLKAVFADDLTDDLVTDEDKALAGDESAWKEIKARLEAERGTALVLLMPRGVGP
                     TRWNAEAKKQTQIERRFMLIGQTSDGMRVLDILRGLQAIRSSGNLPEKSMRVRAKGKM
                     GVLACYAAMFETELALELDQIPLTHRDGPYILNISKVLEVPQAVFLAAQTNPARLTTD
                     RPEAWQPALEAAQRLGWPESQLRIIKRE"
     gene            4961..6544
                     /locus_tag="PLD05_12490"
     CDS             4961..6544
                     /locus_tag="PLD05_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78306.1"
                     /translation="MKMLNLRESVSGCGCCLSRRRFLASCAACAAGAAISQPMGRKIL
                     ASEAKPMRIRILYALHGPVQEVPDWPNIGFDFRPVMERIEKTLKGACPNMEFVSAMAN
                     GPEAAAKIVEEDKANPPDGYIVYQMNCWNRVVQTVADTGKPVLYLDFQFAGSGGFLVY
                     TAAFLRQNRPNVAFVGSSNMEDHVAAVKCFEIIKSGGSVADFVAAVSKTRKDRTPAPG
                     DMTCKPDELKCLSVEETLRQLREKKILAVGGGWGGPRFFEAIQKELGVPVISIGFEEL
                     NEAWKQADKDQAKEIAQGWKKSAAAVMDVSDETLEQSAAMYLAEKALLQRHGANAITI
                     NCLGGFYGGHIHAYPCMGFHELNNQGLIGACECDVRSTITMELITTMTQGRPGFISDP
                     VLDTAKRQIIYAHCVASNRVFGPNGPTNPFQILTHSEDRQGAAVRSLMPEGYMTTTIE
                     LAPDRKQILFHQAKSVGNSLEDRACRTKLCGEPVGDIEKLFTQWDAWGWHRVTCYGDL
                     KEPIFALADKLGWTVIEEA"
     gene            6803..7954
                     /locus_tag="PLD05_12495"
     CDS             6803..7954
                     /locus_tag="PLD05_12495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOP78307.1"
                     /translation="MAKRKKAELSESSAPPVVLDERDQRTLERIQALADHVIEAARAR
                     KDPYLDIPARTLSNVYFNKKKRFIEMGKAKNRRHLFNLAQAKSYMQTMLVASGCKRLI
                     EQGKTTSIRGLFYLLKHTIEGTREETFADQDECDPIIEDIEVTLNALREELHVYATNR
                     GAMVGEITIRDSGDEIDCTRMGSGGYAIPSIVEPNVIQFVRCDAKFVLHVEKDTVWRR
                     FNEDKFWKKHKCILTHGMGQPPRGVRRLLHRLHYELGLPIYCLLDNDPWGYYIYSVIK
                     QGSINLAYESQRMAIPDARFLGLRSTDFERCGLSDSVTIALTDADRKRAKQIMEYPWF
                     KQKKAWQAEIQKLLDNGFKLEVEALISKDISYVTEVYTPQRLRDGDWLD"
     gene            8357..8755
                     /locus_tag="PLD05_12500"
     CDS             8357..8755
                     /locus_tag="PLD05_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP78308.1"
                     /translation="MQVFQPWLLIADDDQRFRETIQNLLEEEGYRTVTAADGEEAVRI
                     VATREVHIVLLDFHMPKLTGLDALKLVRQVRGLLPCILMSAALDANLIEQARQQAVFE
                     ILRKPFSARQLSSVVRSALEQSYRFLPERI"
     gene            complement(9524..>9715)
                     /locus_tag="PLD05_12505"
     CDS             complement(9524..>9715)
                     /locus_tag="PLD05_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78309.1"
                     /translation="GDAQHLDWRRDRVLLEFPRIPGDPHTDFGYPWLVPLENGRWLMF
                     YYHGRKRGHCPIWVTEVTI"
BASE COUNT         2150 a         2706 c         2760 g         2099 t
ORIGIN      
        1 gttcccggac ctgtcccgtt gttatcgatc tgcggcgtac cggtgacttc aaagaattcg
       61 acaaagacca ccgcaccagg cgcattgtga agtaccgctc cgtcggccgg gccagttctt
      121 agcacgatcg ccgagaggac gaaatcccgc ggtgccgtga agacctgccc aaggtcacga
      181 ttccgctgaa aatagccatc gtctttccac acgaggtgtt cggtggccgt atatcgcacg
      241 ctggtatttc caccccgatc ccatagcggt tgtgcaagga tagttcgggg acctggcgtg
      301 aaagtcgaac caatcgcgat caccttaaga ccttcataga cagccaccgt gtcttctgct
      361 tgagcacaac gttcatggtg cctccaggtt ggcccaagga agtacattgt ggccaagaac
      421 gtgaacagca atccgcgacc gtcgaatgcc atattggtcc tcctgttgta ttttccgctt
      481 tttcttttga agcacgtccc acaatctccc ctctcctatt cggcgggaga ggggccgggg
      541 gtgagggtgg ctcgaatcgc tgccggtttt ctcgatcgac tccgacgatc gcttgccctc
      601 accccagccc tctcccggag ggagagggga ctttttggga tcgactctta gttagcccga
      661 agacaggcca ttgtatctat aggataaacg gacggcgaga agatttcgat cttctccaga
      721 tcgaaaccca aaagagcgaa agccaccgag gaggcatcgg tggccttcgc gccttgctct
      781 gtgagcagga cttcgtcgcc agtcaacccg gtcagatcga acacttcggg cgtgaagtgt
      841 tttcaggaaa tcgacatatc ggcgtatctt gggtgcacgt ctcagaagcc gaagtcctca
      901 ggcagcggga cgccttcagc acaactcacc agcagccgca ttacctgggg cttgatctgc
      961 tccgagagga accgcacact tccgtccgag aacaaggcgt ggcaaccgcc cggatggaac
     1021 gagaacggct catcgttcag accgatgttg ttcttgtccc aggtgcaatt gggggccttg
     1081 ccgccgtagg gtgacttgtt gttattgatg acgcgaccat cccaacaggc atcagaaggt
     1141 ccggagacac cgctgccagc ggcatcttga tcggcccatc gccacacggc acggtagggt
     1201 ggccttcccg aatgagggtt accattgtct gtcactgctt ggtcatccgc cgcaagctgg
     1261 ccctgtagat ccagtgttgg atcatgatat ttggagtagg aggcgaattg gacgccggag
     1321 cccggagcac agcggccaac gtcttcgatg atcatgatcg tgttggaagt gccgtccgcg
     1381 attgcggcca ctgaagttgc cgggaccgcc agtgcaccgt taacccgagt ggccaagttt
     1441 cggtgacctg ttgaagggtc aatgtcggtg taaaccgtcg catagtagtc caaccgacca
     1501 taaccggcag ggtccttgta ctccaaaaac ggatttgatg ggcaggtgta caccgcaagc
     1561 tcccggcgaa aggcactcgc atgttcgatg ccagcagttg tgtcgcggta ggtcttgccg
     1621 aggttgatct gatcgtagat gtctttctgt tcgacgaacg gcaaaatgta cgtgaacacg
     1681 gagtggagat ccttcatatc gaaggtcgtc tccggcggcg atgctttgaa gttcgtcccc
     1741 tcgccaccgg taggaaattt cttgtaggca tcgtgaaagt tgtggagagc gatgccgatt
     1801 tgcttgaggt tgttcgagca ctgactgcga cgcgcagcct cgcgggccga ttgaattgcc
     1861 gggaggagca acgcgatcag gattccgatg atcgcgatga ccaccaatag ctcgatgaga
     1921 gtgaacccga agcggctcct catgttcgga gtgtgttttc ggaccttcat tgtgtgttct
     1981 cctttcgtga aagctaccaa actcaccaaa gaccgttccc aatactttct gcacctcttg
     2041 ctttttgttg cgagcgtcat ttgcgagttt gctccaacgc atccggcgtt ggccctcgat
     2101 agttccgctc gcatggtccc tggaagagct cttccacatt tgaaccacac ttgaagatac
     2161 gaatgctgcg ttaagatcaa gttaggaaac ggttaagaaa tggttaaaaa tgcgagcatt
     2221 aattgagact caatatcatg acaccccgct cacgaacccg gcgactcaac cacctaggtc
     2281 gcgagacttt ctctgtgctg tgcgaaggtc cttgacaaaa accggtcttt tcgctgacaa
     2341 cactccgccc tcatcgcttc gctcagtctg cctagcgcgc gggcgagggc tgtgatgacg
     2401 aggacgcggc gtaagttcca gatgagtggc agtggcactc tgcaaagaaa gcaccgcaat
     2461 agcagcgtgg cagccctcgg cgaccgcttt gaccggtcgc gggcggtgat attgggcagc
     2521 tcggttgcct ggcttgtaag gcgatcccct gatctcaagg cgtcatacga cgcggcgcgg
     2581 agcgcggctc actcccgctt tatgattcgc aactgcgatt cgggccagcc taagcgctgg
     2641 gcagcctcca gtgcaggttg ccaggcttca ggtcggtcgg ttgtcaggcg ggcaggattg
     2701 gtctgcgccg ccaaaaatac ggcctgcggc acctccaaca cttttgaaat gttgaggata
     2761 taggggccat cacggtgtgt gagagggatt tgatctagtt ccaaggcgag ctctgtttca
     2821 aacatcgcgg catagcaggc aagaaccccc atcttgccct tggcgcgaac cctcatggac
     2881 ttttctggca gattgcccga ggagcgaatg gcctgcaatc cgcgaaggat atccagtacc
     2941 cgcatcccat cgctggtctg gccaatgagc atgaaccggc gttcgatctg tgtctgtttt
     3001 ttagcttcgg cgttccatcg agtggggccc acgccgcgtg gcatcagcag gacgagggcg
     3061 gttccccgtt ctgcctcaag ccttgccttg atctctttcc atgcgctttc gtcaccggca
     3121 agggccttat cctcatccgt gacgaggtcg tcggtcagat catcggcaaa gaccgccttg
     3181 aggcccttga gccactcctg ccaatctgtc tgatcaagga cctccacaat tgcacggcgt
     3241 acggagccgg ccgccagttc caagcgatag acgggcaggg tgatcttgtc ctggacatcg
     3301 agttttgctc gcgttaaccg aacaccatcc cggaccacac tcacggcatc acgcaacgtg
     3361 gcgggaggaa gatcgccggg ccagccccgg aagcactttt cgcgaattcc cttcagccat
     3421 tccgtttgca tgcgcttcca ttcatcctgg ttttgcggga agacgaccgg ggccgctgga
     3481 acgaaatgtt cgtggatcgt tgtgttgagc tgatctcccg gcaatgtctc aaaaactttc
     3541 agttcctgag gcgtgaggac cttctccacc gggtcgtacg tatcgcgttc cttgcccatc
     3601 aaatggcgat ccaaccagcg aaaggcagcc tcctggatag tcgggatgtc atcgtggccg
     3661 gcagtggtga tgaagatggc gatcttatct tcagcgccat aaagctggta aatctttcgc
     3721 gcctgttcat aaacccgtcg cacaccgtct tcgggaaaaa tgggatcatg gtccccgttg
     3781 tcgatcagca gcggtcgggg agcgaccaag gccgccacca ttgggtagtc ccatcggtag
     3841 gtgttgacga tgtacatgca gtcgcaatgt ccctccacac acccgtcgat aacgtgattt
     3901 tgaaggtctg taatgcccgc cacggggacg gccgccttaa tgcggggatc gatcgccgcg
     3961 atccaccaac tgtaggcacc accaccgctg cggcccgtga caccgatccg ttccccgtca
     4021 atttctgggc gggattgcag gtaatccaat gcccggacac agttccacgc ttccaccccg
     4081 gccggtgtgt acccgcgatt attccaccac cacatcccgt aacgataggt tccgtgatga
     4141 attccctcga tttcgcccaa ttgcaggcta tctagcacca gacaagcgta tccgtgacgg
     4201 gcaaaccaca cggcgtggaa gtgataatgg gccttattac cgtaactgac gttgtcaatc
     4261 ttgacttggg catgaccaca cagataaagg acagccggta cgggcttggt gatattcttg
     4321 ggcaaataga gattggccgt gacataaagc ccgggcatgg attgaaaatg caacttctcc
     4381 acgcggaaat catctcgctc caacatgccg gtgatcgtgg ctttcaaatc tgtctttttc
     4441 ggccagggat caaggccaag catttcccgc aactggcgtc gatactcctc acagcgggct
     4501 tgccagtctt cgagggtctt gatgtcggcc agacaagctg cctggagctt gccgacttcc
     4561 acctggaagt aatcggctag catcgcatcg ccgggcaagg gaccaagggg tttccattcc
     4621 gggcgcggct gagcaaagat caatcctgtc gtggctacgc cgcccaaaaa gaggatgctt
     4681 acatacagga gcgccttgga atgaagatga caccgaaaca tgatttcgac ctcccgctga
     4741 cctaccagtt aacctgtcgc cgatcacaaa accacagata ttgaaacagg ttcacagggc
     4801 cgaagcaagt cggttgcgtt gaatagttga ctaaagcaat caagccaatc atcttaatgc
     4861 ttgagtttac cggcaggatg ggataccgtg atgatgtgtt tacggtagag cttccgtttg
     4921 ggttcccgtt ggtcaagttc caagtatcga gggtggtaag atgaagatgc tgaatcttcg
     4981 agaatcagtt tcgggttgcg ggtgctgcct ttcacggcgt cgtttcttgg cgagctgcgc
     5041 tgcctgtgcg gccggggcgg cgatatctca accgatgggg cgaaagattc tggccagtga
     5101 ggccaagccg atgcgaattc gcatccttta tgcccttcac ggtccggtcc aggaggtgcc
     5161 tgactggccg aacattggct tcgacttccg cccggtgatg gaacggatcg aaaagacact
     5221 taagggtgcc tgcccgaata tggaatttgt ctccgcgatg gccaatgggc ccgaagcggc
     5281 agctaagatc gtggaggaag ataaggctaa tccgccggac ggctacattg tctatcaaat
     5341 gaattgctgg aaccgtgttg tgcaaactgt ggccgacacg ggcaaacccg tcctttacct
     5401 cgacttccaa tttgcaggca gcggcggatt cctggtctat acggcggcct tcctgcggca
     5461 aaataggcca aatgtggctt ttgtcggctc atccaacatg gaagatcacg tagcggccgt
     5521 caagtgtttt gagatcatca aatcgggcgg gtcagtcgcc gactttgtgg cggccgtgag
     5581 caagacgcgt aaagacagaa cacctgctcc cggcgacatg acctgcaagc cggatgaact
     5641 gaagtgtctc tcggtcgagg aaaccctccg gcaacttcgg gagaagaaga ttctagccgt
     5701 cggcggtgga tggggaggcc ctcgcttctt tgaagccatc caaaaagaat tgggagttcc
     5761 cgtaatcagc attgggtttg aagaacttaa cgaggcgtgg aagcaggctg acaaagatca
     5821 ggccaaggaa atcgcacaag gttggaaaaa atccgccgcg gccgtgatgg atgtttcgga
     5881 tgagacattg gaacaatcgg ccgctatgta tctggcggaa aaggccctac tgcaacgtca
     5941 cggcgctaat gccattacga tcaactgctt gggtggtttt tacggcggtc acatccatgc
     6001 ctatccctgc atgggattcc acgaactgaa taaccaggga ctcatcgggg cctgcgaatg
     6061 cgatgttcgc tcgaccatca ccatggaact tatcaccacc atgacccagg gacggcccgg
     6121 cttcatctcg gacccggttc tcgacacggc caagcgacag atcatttatg cccactgtgt
     6181 ggcgtccaat agggtgtttg gtccgaacgg gccgacaaat cccttccaaa tcctcaccca
     6241 ctcggaggat cggcaggggg ccgctgtccg ctctctcatg cccgagggtt acatgacgac
     6301 gacaatcgaa ctggcgccgg accgcaagca aattctcttc catcaggcca aaagtgtggg
     6361 caactccctg gaagatcggg cttgccggac caagctgtgt ggcgaaccag tgggtgacat
     6421 cgaaaagctg tttacccagt gggacgcttg gggctggcac cgtgtgacgt gctatgggga
     6481 cctcaaagag ccgatcttcg ccctggccga caaactaggc tggaccgtca tcgaagaggc
     6541 ataaccgacg cctgagggat aagcgcgaaa gcatcgcccg gcagcggagg ggcgttacca
     6601 aagacgcagc cctccgcgtt tggtttttgt gaaagaaaac gcccttttcc aaatattcag
     6661 gtcgccgtac attgggagta tgacggcgtg agggccacgc cgcgatggcg gtatgattca
     6721 gtgattaccc gcggtacagg gccaagggct cgctgtcctt ccggtaacct caaccaaggc
     6781 gattagtcat cacggaaaga gtatggcaaa gcgcaaaaag gccgaattgt cagaaagttc
     6841 tgctccccct gtcgtccttg acgaacgaga tcagcggacg ttggaacgca tccaggcttt
     6901 ggcggaccac gtgatcgagg ctgcccgggc ccgcaaggat ccctacctag acatccccgc
     6961 ccggaccctt tctaacgtct atttcaacaa gaaaaaacgt tttatcgaga tgggtaaagc
     7021 caagaaccgg cgccatcttt tcaacctcgc tcaggccaaa agctatatgc agaccatgct
     7081 ggtggccagc ggttgcaaac ggctcatcga gcagggaaaa acgaccagca tccgtgggtt
     7141 gttctatctg ctcaagcaca ccatcgaggg tacgcgggag gagacgttcg ccgaccagga
     7201 cgagtgcgat cccatcatcg aagatattga agtgacgcta aacgctcttc gggaagaatt
     7261 gcacgtctat gccaccaacc gcggggctat ggtcggggaa atcaccattc gtgacagcgg
     7321 agacgaaatc gactgcacgc ggatgggctc gggtggctac gccattccat cgattgtgga
     7381 accgaatgtg atccagtttg ttcgatgcga tgcgaagttc gttctccacg tcgaaaagga
     7441 cacagtttgg cgtcgcttca atgaagataa gttctggaaa aagcacaagt gtatcctgac
     7501 ccacgggatg ggtcagcccc cgcgaggtgt gcggcggctg ttacaccgcc ttcattatga
     7561 attgggatta cctatctatt gcctcctcga taatgacccc tgggggtact acatctacag
     7621 cgttatcaaa caggggtcga tcaatctggc gtatgagtcg cagcggatgg ccatcccgga
     7681 tgcccgcttt ctgggcctac gttccacgga tttcgaacgc tgcggccttt cggatagcgt
     7741 cacaattgcc ctgacggacg cagaccgcaa acgggcaaag cagatcatgg aatatccctg
     7801 gtttaaacag aaaaaggctt ggcaggcgga aatccaaaaa cttctcgaca acggcttcaa
     7861 attggaggtt gaggcactca tctccaaaga tattagctac gtcaccgagg tttatacccc
     7921 tcagcgactc cgcgacgggg attggctcga ttgaaaagat ctcccgcacg gctccttttg
     7981 gccaagagtt gccggagcag aggctacgtg cgcccggcgc ggcatcagag gaagacgaac
     8041 ttcggtcatg agccacccct gcgctggttc tccatggttc ggttgtgacc gggcgcggtc
     8101 cagggcaccc acacggttcc ggtaggcaag gcaagccagg cagtttttct tggcggtggc
     8161 tgtcagtgtg gcagtccgtt tttgtgcgta gagaacgctc ctaatcaccg atcggccagt
     8221 acgctggagg gatcaggcag ggattctacg gttttggaag gcgttaagcg cgacataccc
     8281 accatttggg gatacttctc gccttggcac aggagttgct tcaagctatg attggatgtc
     8341 gcgtggaggc aaaagcatgc aggtgtttca gccctggctc ctgattgccg atgatgatca
     8401 acggttccgg gagacgatcc agaacctcct ggaggaggag gggtaccgca cagtgaccgc
     8461 cgccgacggg gaggaagccg ttcggatcgt cgcgacccgg gaggtgcata ttgtcctgct
     8521 cgatttccac atgcccaagc tgacggggct ggatgccctc aagttggtta ggcaagtccg
     8581 gggactgtta ccctgcattc tcatgtcggc ggcccttgat gcgaatttga tcgaacaggc
     8641 ccgtcagcag gcggtgttcg agattctccg caaaccattc tccgctcggc aactttcctc
     8701 ggtggtccga agcgctctag aacaatctta tcgttttctt cccgagagga tttgacggcc
     8761 accctcttcc caacgccgga tagcggcagc accaccgcgg ctggcaccaa aacctagatc
     8821 ataggccatc accctgcaat aacggacgag cgatggggga ttcgactggt gtgacgtaaa
     8881 ttattatcaa aaacaacttg tgtcaattcg acccatccaa ttggcacaaa atccggaaca
     8941 cttgatgata actcgccgat gagcgatgga cgtgacgacc gtgggatatt caccattgac
     9001 aaccccggcg gatgggacca tgaagccgca cttgcgacat aggcgcggtt accagaattg
     9061 tggccacggt tacctataga ggttcaccgc tagattgtgg ccatcgcaga gggatctgct
     9121 gccaccgaga caggaatgcg tgaagcaaga cacctcaccg caaccgttgt tgcctcatca
     9181 aacacgcgcc gccttgggca ccgttttggc gaagcggcat ccggttgttt cttgttgcgc
     9241 attctaggat ggtagggcct gtaccgccgt gggcgccagt cgtgtttgcc catgggaatc
     9301 acgttgaaca ggaccgcccc gaggattgtt ccaatccggg caaccacagg gggttgcccg
     9361 tacacacccc atcaaaacag taggggcagg cccccgtgcc tgcccggacc ccgtacgtac
     9421 tcgacgcccc cgtgcctgcc ggagaccgcg gacgtcccga attgcacgta ggacgcgggg
     9481 gaccgcgtcc ctcccattcc catcggcgga cacgaagtta gattcagatt gtcacttctg
     9541 taacccaaat cgggcagtgg cctcttttgc ggccgtgata gtaaaacatc agccatcggc
     9601 cgttttctag cggaacgagc caaggatacc cgaaatcggt gtgggggtcg ccggggatgc
     9661 gcggaaattc gagcaagacg cgatcgcgtc gccagtcaag gtgttgagcg tcgcc
//
LOCUS       DAOSUT010000151         9674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3834_length_9674_cov_9.728038, whole genome shotgun sequence.
ACCESSION   DAOSUT010000151 DAOSUT010000000
VERSION     DAOSUT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_9674_cov_9.728038"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2205)
                     /locus_tag="PLD05_12510"
     CDS             complement(<1..2205)
                     /locus_tag="PLD05_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOP78310.1"
                     /translation="MTLDEAINRLAQYGCEPQETRKGWVARCPAHDDRKPSLSVSQGR
                     DGRVLMKCHGGCPPEAVCAKLGLTMADLMPPKDNTRTSGARRNGKAKREMVGQVFPKA
                     RDALAKLRQTLGRETAKWTYHDANENPVGLVCRWDTPHGKEIRPISYYPDGWRITQMP
                     QPRALYNLPAVLKADPERRVWIVEGEKCADALASLSLLATTSSGGANAAAKTDWSPLR
                     GRRVIIAVDNDDPGRRYGQAVAKLAHEAGAREIRVLDLGQRFPNLPQGFDVADLVENP
                     TAIGLAEGTPADELRKLLEEWADEAPTWKPDDSEPNAVPLVERYEPFPVDALPEPLRR
                     FVVEGAEALQCDHAHLALPLLTVVAAAIGNTRRLRLKSDWLVPSMLWAVVVAESGGMK
                     TPALRLVLRPVRERQHAALERYEDAKRKYESAVLRYEKDLAEWRRSKDTMMGPPEKPE
                     PPQPERFIVSDTTLEALAPLLLENPRGLLLACDELSAWLGSFDRYVTTKGGDVGRWVS
                     IHSGESITVDRKTEPRTISIPYPCVSVCGGVQPATLTRLMGASYRDCGLAARILWAYP
                     PKRRKRWTEAEIDPATHSAITRLLDRLYTLQPTTDEKGRPCPVILDLTPDAKELWANY
                     YDAHGTEWLDLTGELASAWAKLEEYAARLALVLHCVRVVTRDATTDAVDAVSMQSGIT
                     LATWFKREARRVHALFTETDEEADQRRLIEWIEARGGAATVRDLTHGLRRFRN"
     gene            3957..4529
                     /locus_tag="PLD05_12515"
     CDS             3957..4529
                     /locus_tag="PLD05_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOP78311.1"
                     /translation="MKVFTTGQVAKICKVAPRTVSKWFDSGRLKGYRIPGSQDRRIPR
                     EYLIKFLKEHGMPLGELEDEMTAKVLLVAQDQVLIENLKAELPPERSFKVAVASSGFD
                     AGIQAESFRPDCIVVDFSIGRTEALQICQHVRRNTEFNDVILIALLPDDGNAMSFDRS
                     GINETFKKPFDAHLLAERIRTLVGAKKELV"
     gene            complement(4685..7354)
                     /gene="topA"
                     /locus_tag="PLD05_12520"
     CDS             complement(4685..7354)
                     /gene="topA"
                     /locus_tag="PLD05_12520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOP78312.1"
                     /translation="MAKKKAAIPKSSGTEKQKKLVIVESPTKARTISRFLGDEFVIEA
                     SLGHIRDLPEGAKQCPPEIRSKPWAYLGVDIEHDFQPIYVIPKEKAKQVQQLKKAMKG
                     ASELFLATDEDREGEAISWHLCQVLQPNIPVRRLVFHEITRDAILAALASPRGIDENL
                     VRAQEARRILDRLFGYEISPLLWKKIGSRLSAGRVQSVAVRLIVERERQRMAFVSASY
                     WDLIADFATQRNEQLSAELVSVHGKALPTGKDFDPNTGQLKTPGLLLLDEKAAEELIQ
                     QLRAKGAQSPSGEIQGVVIQAEEKHYTSRPAPPFTTSTLQQEANRKFGFTARYTMQLA
                     QRLYENGYITYMRTDSTNLAQEAINAARNLILRQYGEDYLPDRPRYYQTRVKNAQEAH
                     EAIRPAGHEFALPESLRGELSPDEFRLYDLIWKRTIACQMADARGRRMTVQIDVDGLV
                     FQATGKTIDFPGFLRAYVEGADDPEAELADRETILPPLQTGEHVVLRDVEAKGHVTQP
                     PSRYTEATLTRTLEELGIGRPSTYATIIDTILERNYVTKKNNSLVPTWTAFAVCQLLE
                     RHFAEMVDYSFTAQMEDELDAISRGELNWVDYLREFYFGNSRPGLKKLLETKIAEIDA
                     REINRIPLRSPGSAADNEKPIFVRIGRYGPMLEWGEKRIRLPEDLSPDELTLDKAKEL
                     FSKAEEREQPIGHCPQTGLPVYLRSGRYGWYLQRGDGHDSKSAERVSLPPDVHPESLD
                     LSTALRYFELPRKLGEHPETGEPVEVWIGRFGPYVKCGQETRSLPADKSPFEITLEEC
                     LALLNAPKASSRRTITELRQLGVSPETGQPVRLMNGRYGPYVTDGQTNATIPREISPD
                     SVTLEQALQWLTEKAAQGPRKRWRRTSGKRTKK"
     gene            7971..>9674
                     /locus_tag="PLD05_12525"
     CDS             7971..>9674
                     /locus_tag="PLD05_12525"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOP78313.1"
                     /translation="MASPFKVFRKNAKVLLVGLFLLSMISFVVIPSFLQWLQTRQGRA
                     VQSVVSTKKFGELSEIDIHNLWMEHNTVRRFLEALGRRIDESRGSSFRIRYFLAVMGE
                     ATEEQLVQTWLFSQYAEIAGVKVTDEVINEFIRGLINASGVSISPADLAQLLSQNQIQ
                     EDTLFRGLRREILVLRYLQLAGFDMFSLQPGFAGETPAMRWEYFLRFNRLAAIELCGI
                     PVERYYERAPTPNDQQVREYFESHKEKLPDPSSPEPGFKIPAKVTVEYLKGDLQKWLV
                     NVKLSDEELRNYYEQHKDEFVARPASSPTTTSESVNTPPSNSSQPSGVTPPAGETPTT
                     PKDDNARADDSTANAKQESNPLPSESQSAEKTSEQPSTDEKKTNGPSTHEGTGNAHAV
                     SPFRLVSLVEDSAGSETEKVDSTTDAPAEPRAEGMGDSQESASQQEAPSQMAASSEGQ
                     SPSQEPSMGQAEQDKQGQSQPSATAEPEKAPQEARTETAAPPPGTSVPTEGPVMTTPT
                     APEVSSGAVSQSKEPEGTASKYQPFEEVKERVRAMIISERIKQALDELEEVMRDYQTD
                     WA"
BASE COUNT         2048 a         2757 c         2645 g         2224 t
ORIGIN      
        1 gttccgaaac cggcgcagcc catgggttag gtctcggact gttgccgctc caccccgggc
       61 ctcgatccac tcgattaacc gccgctggtc ggcttcttcg tccgtctccg tgaacaaagc
      121 atggacgcga cgggcctcac gtttgaacca cgttgccaac gtgatgccgg attgcatgct
      181 tacggcgtcc acggcatccg ttgtcgcatc acgggttacc acacggacgc aatggagcac
      241 cagggccaag cgggcagcgt actcttccag tttcgcccat gccgacgcca gttcgccggt
      301 taagtcaagc cattctgtgc cgtgggcgtc gtaatagttt gcccacagtt ccttcgcgtc
      361 cggcgtaagg tcgagaatca ccgggcatgg cctccctttt tcatctgtgg tcggttgtaa
      421 ggtgtacaac cggtcaagta gtcgggtaat ggccgaatgc gttgccgggt caatctcggc
      481 ttccgtccag cgttttcggc gttttggagg gtaggcccac aatatccggg ccgctagtcc
      541 acaatcccgg tacgacgccc ccattaatct cgttaaggtt gcgggttgga cgccaccgca
      601 aacagacacg catgggtatg gaatagatat cgttcgcggt tccgttttgc ggtcaaccgt
      661 gatggattcg ccggagtgaa tcgaaaccca ccgtccaacg tcaccacctt tggtggtgac
      721 ataccggtcg aatgacccaa gccatgcgga caattcgtca caggccaaca gtaacccgcg
      781 cggattttcc agtaacaacg gggccaatgc ctctagcgtt gtatcgctaa caatgaatcg
      841 ttctggttgt ggcggctctg gcttttcggg cgggcccatc atcgtatcct tagaccgccg
      901 ccactctgcc aggtcttttt cgtaccgcag tacagcgctt tcatacttgc gcttggcatc
      961 ttcgtatcgc tccagggccg cgtgttgacg ttcccggaca ggccgtaaga ccaaccgcag
     1021 cgccggcgtc ttcatcccac cggattccgc cacaacgaca gcccacagca tagacggtac
     1081 aagccaatca ctttttaacc gcagccgtcg ggtgttaccg atagccgccg caaccaccgt
     1141 cagcaagggc aacgcaagat gagcgtggtc gcattgcaat gcctccgcgc cctccaccac
     1201 gaatcgccgc aacggttccg ggagcgcatc caccggaaac ggttcgtatc gctccaccaa
     1261 cggcacagcg ttaggctcag aatcatccgg cttccaggtc ggcgcttcgt cggcccattc
     1321 ttcgagtagt ttccgcaatt catccgccgg tgtgccttcg gctagtccaa tcgccgtagg
     1381 gttttccact aagtcggcca catcgaagcc ttgcggtaga ttgggaaacc gttggcctag
     1441 atcaaggacg cggatttccc gggcgccggc ttcgtgggcc agtttggcaa cggcttggcc
     1501 ataccgccgt cccgggtcat cgttgtccac ggcaatgatc acccgccgtc cgcggagagg
     1561 gctccaatcg gttttggcag ccgcgttagc gccgccgctc gatgtggtgg caagtaggct
     1621 aaggctagct aacgcatcgg cacacttttc gccttcgact atccaaaccc gcctttcggg
     1681 atcggccttt aacaccgcgg gaagattgta caaggcccgc ggttgcggca tttgggtaat
     1741 ccgccaaccg tcggggtaat aactgatggg cctaatttcc ttgccgtgtg gggtatccca
     1801 tcgacacact aggccgacag gattttcgtt ggcgtcgtga tacgtccatt tagccgtttc
     1861 acggccaagt gtctgccgaa gtttggccag cgcgtcccgg gccttaggaa acacttggcc
     1921 caccatttcc cgcttcgcct tgccgttgcg tctcgctccg ctggtccgcg tgttgtcttt
     1981 aggtggcatc aagtcggcca tagttaaccc caactttgca catacagcct ccggtggaca
     2041 cccgccgtga cacttcataa gaacacgccc atcccgccct tgcgacaccg acaaacttgg
     2101 cttgcggtcg tcatgggcgg gacagcgggc cacccagccc ttcctggtct cttgcggctc
     2161 gcacccatat tgcgctagtc ggttaatcgc ttcgtcaagg gtcatacagt ctgccccctt
     2221 tgatgttgcc ttgactgcgc agtatcggcc ccgttgcgca gtatcaccgt ggttctgccg
     2281 tatcgccttg gtagtccggc atcgccttag ccgttggttt gattctgccc cccttgctct
     2341 cgcagccacg cctttagggc ctcaccaggg agaggcactg tctttctgcg gccactccca
     2401 ggagatacac tcgggatttt cttttcctgc ccccatcgcc acgctgtgtc gccggaaatt
     2461 cctaaaagcc gggccacttg tggaagacgg taggctaacc tttctgacat gtccatgatt
     2521 ctgccccttt ctgaaacaag ctacgtcttt ggaacttatg tgccctctat gtatttctgt
     2581 ccctgagctc agcccaagtg cgcgggtttt caaaaaaagt gctatatata gcgtgctcgc
     2641 caccagaaag agataggacc agcgctaatg aatgggcagg ttaactagct aacatgtgcc
     2701 gcggcagata cggttcccaa agcaatccaa tcattactat gttgatacgc aaaacaaact
     2761 ttactcgcca ttgcgattgt tcccgcggcc cctctcgaat cgccttcact gggaaggacc
     2821 cataaacgtc aaagatcagc gccggctatc cggctttccg cggacaaacc acgtccgtcg
     2881 gcccacagcg aggaccatcc atccgcgtcc gtggaggaag tgcagcaatg tatgcagcag
     2941 tcccaccccc acgaatgcta taagtgtcga tatatcaata acttgcgtcg taatgttcga
     3001 gataagaagt cagcagctca aaggcgatca gtgagcgttt tcgccgctga cgagcttata
     3061 aacaggtcct tttctttgcc tttcttcttc gaccattcgg tcggaggaga gttatgggcg
     3121 gcggaagtcg ctctgtggcc atgtggttca aaaagcatat ccgcttggcc gggatgagct
     3181 gatcagcaaa gtcttcctaa aggagaaagt cgcaagactt ccccggactt ctcgcatttc
     3241 tccccacatc gcttgtaaaa acaacacggc acagggtggg caggtatgtc gggagaaacg
     3301 ggcgagccgc gtagtctgga tggagtacga atgctgaggt gagtttttat tacattcaaa
     3361 ctgtcccaaa taatccatct tttcgaacga accctcgaat tcgctacaac ccgtcaaact
     3421 tcttaaagag tttctcattc tcgattcgaa acaatcaatc ggagggccgt tgtctccgag
     3481 atgagacgaa atggatggag ggccatccga gaatcgtccc agggggattg actggggaac
     3541 cacatttgac cgtttgcgcg gagagtgtca aatcctgcgc gaagatggtt cctgtctttt
     3601 gccccgggga caccagacca gcaggagatg accgttatag aggtcaccga ggtcggactg
     3661 tccttcccca ttttgcgaat tttcactgtt tttcggcggc ccggtttgcc gatgtcatta
     3721 ctgaactgag gcgcgggtca gatggagctg agccacggcc gaaacttttg gaacagggtg
     3781 ttcccagcgt tccgagaccc agcggtttgc gaaattgagc caagtgggag tcaggaccac
     3841 acgaaggagc gtgtggtgag gcgactcgca aacacgggaa cgcggtgtgg aaggtcggtt
     3901 ggtgaaaccg atagggatta gtcaactcaa gcgagtgaca gtttggagag cctgcaatga
     3961 aggtcttcac tacaggtcag gttgctaaga tctgtaaagt tgccccgcgc accgttagta
     4021 agtggtttga ttcgggccgt ctcaagggat accgcattcc gggttcgcag gatcggcgta
     4081 ttccccgcga atacctgatc aagtttttga aagaacacgg aatgcctctc ggtgagcttg
     4141 aggacgagat gaccgcgaag gtcttgctcg ttgctcagga ccaagtgctc atcgagaact
     4201 tgaaagcaga gttgccgcct gaacgttcct tcaaggtggc cgttgcttca agtggttttg
     4261 acgccggaat tcaagccgag agtttccgcc ccgattgcat cgtggtggac ttttctatcg
     4321 gtcgcacgga ggctttgcag atctgtcagc atgttcgtcg taacaccgag tttaacgatg
     4381 tgatcctgat tgcccttctt cccgatgatg gcaatgccat gagcttcgat cgctcgggca
     4441 tcaatgagac gttcaagaag cctttcgatg cccatctgct agccgaaagg attcggacgc
     4501 tcgtgggagc gaaaaaggaa ctggtgtaag ccgaaggagt cggcctatcc cgatcgccgg
     4561 ttcccgacaa cccgccgcgg aaggaccccc gcgacggata tttcccgcag cccgcgtgct
     4621 gcgggttttt tatttcttgt tacctggtgt cgagccagca cgcccacgcg gtcaccaccg
     4681 ggggttactt ctttgttcgc tttcccgatg ttcggcgcca tcgctttcgc ggaccttggg
     4741 ctgccttttc cgtcaaccat tgcaaggcct gctctaaggt caccgagtcg ggtgagatct
     4801 cccgtgggat tgtggcgttc gtctgaccat cggtgacgta gggaccgtaa cggccattca
     4861 tcagccgaac aggttgccca gtctccgggg acacacccaa ctgccgaagt tccgtaatgg
     4921 ttcgacgcga tgatgccttc ggcgcgttga ggagggccaa gcattcctcc agcgtgattt
     4981 caaatgggga tttgtctgcc ggcaatgaac gcgtttcctg gccacacttg acgtacgggc
     5041 caaaccggcc gatccagact tccaccggtt cgcccgtttc gggatgttcc ccgagcttgc
     5101 gcggcaactc aaaataccta agggccgtgc tcagatcgag cgattccgga tggacatcgg
     5161 gaggtaacga cacgcgctcg gctgatttgc tatcgtggcc atcaccccgc tgaaggtacc
     5221 acccgtaccg cccactccgc agatacaccg gaagccccgt ttgcggacag tggccgatcg
     5281 gctgttctcg ttcttccgcc ttgctgaaca gctccttcgc tttgtctaat gtcagttcat
     5341 caggcgagag gtcttcaggc aggcgaatcc ttttttcacc ccattctaac attggtccgt
     5401 agcggccgat ccgaacgaaa atcggttttt cgttgtcggc ggccgaacca ggcgagcgaa
     5461 gcggaatccg attaatctct cgagcgtcga tctccgcgat cttcgtctca agaagttttt
     5521 tgagtcccgg ccgtgaattt ccaaagtaga actctctgag gtaatctacc cagttcaatt
     5581 cgccacggct aattgcatca agctcatctt ccatctgggc agtgaaggag tagtccacca
     5641 tctccgcaaa gtgcctttcg aggagctggc agacggcgaa ggccgtccag gttgggacaa
     5701 gcgaattgtt cttcttggtg acatagtttc gctcgaggat tgtgtcaatg atcgtcgcgt
     5761 aggtactggg acgtccaatg ccaagttcct caagcgttct agtcaaggtt gcctcggtgt
     5821 agcgactcgg gggttgggtc acgtgtccct tggcctccac atcgcgcaag acaacatgtt
     5881 ccccggtctg tagcggtggc aaaatggtct cccggtcggc caactcggcc tctgggtcgt
     5941 cggctccctc cacataagcg cggaggaacc ctggaaagtc gatcgtcttg ccggtagcct
     6001 gaaagacgag cccatccaca tcaatttgca cggtcatccg ccggccgcgg gcatccgcca
     6061 tttggcaggc aatagtcctc ttccaaatga gatcgtagag tcgaaactca tcgggcgaaa
     6121 gttcgcctcg caaagactcc ggcaaggcaa actcatgccc ggcagggcga atcgcttcgt
     6181 gcgcctcctg ggcgttcttg acccgagtct gatagtaccg gggccgatct gggagatagt
     6241 cctcgccata ttgtcggaga atcagattgc gcgcggcatt gatggcttcc tgggccaggt
     6301 tcgttgaatc cgttctcatg taggtgatgt acccattttc gtaaagccgc tgggccaatt
     6361 gcatcgtata acgggctgta aaaccgaact tgcgattggc ctcttgttga agggtactcg
     6421 tggtgaaggg cggagccggc cgcgatgtgt aatgcttttc ttcagcctga atgacgactc
     6481 cctgaatctc tcccgacggg gattgggcac cttttgccct tagttgttgg atcaactcct
     6541 cggcggcctt ctcgtcgagc agaagtaacc ccggcgtttt cagttggcct gtgttgggat
     6601 cgaaatcctt gcccgtgggt aaggcctttc cgtgcacact caccaactcg gctgataact
     6661 gttcgttgcg ctgcgtcgca aaatccgcaa tcaaatccca gtaactggcg ctgacaaacg
     6721 ccattcgctg acgctcacgc tccacgatca gccgcacggc cacgctctga actcggcctg
     6781 ccgaaaggcg cgagccaatc ttcttccaca gaagaggcga aatctcgtac ccaaataacc
     6841 ggtctaagat acgccgggct tcctgggcgc gaacaagatt ctcgtcgatt ccccgggggg
     6901 aggccaaggc agccaagatc gcgtcgcggg taatttcgtg aaacactaac cgccgcacgg
     6961 gaatgttcgg ctgcagcacc tgacacagat gccagctaat cgcctcaccc tcccggtctt
     7021 cgtccgttgc caggaaaagc tccgaggccc ctttcatggc tttcttgagc tgctgaacct
     7081 gtttggcctt ctccttcgga atgacataaa tcggctggaa atcgtgttca atgtcaacac
     7141 caagatatgc ccaaggtttg gagcggattt ccggtggaca ctgtttcgct ccttcgggca
     7201 ggtcgcgaat atggcccagg ctcgcctcaa tgacgaactc gtcgcccaag aaacggctga
     7261 tcgtgcgagc cttggttggc gactctacaa tgacgagttt cttctgtttt tcggtcccag
     7321 aagatttggg gatagctgct ttcttttttg ccatttctca ccaaaaatcg gatattattt
     7381 gctcgataac accctcgcct acaaatttac tggcctaagg gcttgcccgt taccatttgc
     7441 ccctaataca ttctttgccg tgccaaggag ctgggcgcac ccgaagtacc acccgatttg
     7501 tgataacctg tattggaaat ctttccaaga ccacctggaa acggaaaggt gcactctgga
     7561 cctatataac ccaccctagc ctgggggatt cccgtgggcg gacgctcggg acgtcgttta
     7621 aatccgccaa atagcgcgag ggaaaactgc tttaacccgt ctcaaacacc gcacgacagg
     7681 gccgtagcaa actggcaccc aaattctttc tgaagcgcat cgtgggcttt ttgacaaggc
     7741 ttgccgaaag cggagatggc atttccattt cgtcgccgag aacttagaat gatggttctt
     7801 ggcagtttct gcaattcctc gcctccaggg gcgaggatgg atatcgctac ggcccggccg
     7861 acgggccatt ttgtggttgc taaacgcccc cttgcgggtg gagttcgggt taagttggag
     7921 cctcgttctt gacgaggggt tgaatttagc attacggaag cgatcccgtt atggcaagcc
     7981 cgtttaaagt tttccgcaaa aacgcaaagg tcctgctggt cggactcttt ctcctttcga
     8041 tgatcagctt cgtcgtcatc cccagctttt tgcagtggtt gcagacccgc cagggccgcg
     8101 ccgtccagtc ggtcgtttcc acgaagaaat tcggcgagct gtcggaaatt gacattcaca
     8161 acctttggat ggagcataat acggtccggc ggtttttaga ggcgctcggt cggagaattg
     8221 acgaatctcg cggaagttct tttcgcattc gctattttct cgccgtgatg ggggaggcaa
     8281 ccgaggagca actcgtgcaa acctggctgt tttcgcagta tgccgaaatt gccggagtta
     8341 aggttacaga tgaggtgatc aacgaattta ttcgggggct gatcaacgcc tcaggtgtgt
     8401 cgatttcccc ggctgatttg gcccagttac tgtctcaaaa ccaaatccaa gaggatacgc
     8461 tgtttcgcgg gcttcgacgg gagatcttgg tccttcggta tctccagctt gccgggtttg
     8521 acatgtttag tttgcaaccc ggctttgctg gtgaaacccc cgctatgcga tgggagtatt
     8581 tccttcgctt caacaggttg gccgcaattg agctatgtgg gattcccgtc gaacggtatt
     8641 acgagagagc accaactccc aacgatcaac aagtgcggga gtattttgaa tcgcataagg
     8701 aaaagttacc ggatccatcg tctcccgagc ctgggtttaa aatccccgct aaagtgacgg
     8761 tggagtatct gaagggagat cttcagaagt ggctggtcaa tgtcaaactg tccgatgagg
     8821 agctgcgcaa ctattacgaa caacacaagg atgagtttgt tgcgcgaccg gcttcttcac
     8881 caacgacgac aagcgagagc gtgaacacac ctccgtctaa ctcatcgcag cccagtggcg
     8941 taacaccgcc agctggggag acgcctacca ctccgaagga tgacaacgcc agggccgatg
     9001 acagcactgc gaacgctaaa caggagtcga atccccttcc cagcgagagt caatctgccg
     9061 agaagacttc cgaacaacct agcaccgatg agaagaagac aaatggtccc tcgactcatg
     9121 agggtactgg caatgctcat gcagtgagcc ccttccgtct ggtatcattg gtcgaggact
     9181 cggcaggttc ggagaccgaa aaagtagatt cgaccaccga tgctcctgcc gagccccgtg
     9241 ccgaggggat gggcgattcc caggagtctg caagtcagca ggaggccccg agtcaaatgg
     9301 cggcatcgtc ggaggggcaa tcgccgtccc aagaaccttc gatgggccag gccgagcaag
     9361 acaaacaggg ccaatcgcag ccgtcggcaa ctgctgaacc agaaaaggca ccgcaggagg
     9421 caagaacgga gactgccgct ccgcccccgg ggacttctgt cccgacggaa ggtccggtca
     9481 tgacgacgcc aaccgcgccc gaagtttcat ccggagccgt atcccaaagc aaggagccgg
     9541 aaggaacggc ttcaaagtac caaccctttg aggaggtcaa ggagcgagtt cgggccatga
     9601 tcatttcgga acggattaag caagcccttg atgagctcga agaggtgatg cgggattatc
     9661 aaacggattg ggcc
//
LOCUS       DAOSUT010000152         9510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3890_length_9510_cov_8.858065, whole genome shotgun sequence.
ACCESSION   DAOSUT010000152 DAOSUT010000000
VERSION     DAOSUT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_9510_cov_8.858065"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..795
                     /locus_tag="PLD05_12530"
     CDS             82..795
                     /locus_tag="PLD05_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78314.1"
                     /translation="MPVLGAPYRAIYAWLAAALGWNIAVLAFALGTYYVGRFDWSFTL
                     FLLPCLVIGLTLLGAFFRQWMATSAVGETIVEISDFPLLCGHRYRVYLKQTGWLRINW
                     LEMLLVCEEEATFQHGTNTRRECRRVWQKTLWRHERFEILRSVPFETEAEIEIPHGMM
                     HSFRSPHNEVRWRLYVRGSAQDWPNFERVFPLVVLPGPERERRPDSRPGREGLSSETP
                     SDEESPQQVNPGQASETYS"
     gene            792..1193
                     /locus_tag="PLD05_12535"
     CDS             792..1193
                     /locus_tag="PLD05_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78315.1"
                     /translation="MTEPRILIHLDGKKTVYEPGEVLAGQVRIEPLPLQDIKAVELSI
                     LWYTDGKGDQDLHVRYFRRWSQEGDLPLDLREPLRFQTVLPQTPLSYDGLIIRIRWCV
                     RVRVFRARGRELVEEVPFRLGRVAPARVRLQ"
     gene            1190..1852
                     /locus_tag="PLD05_12540"
     CDS             1190..1852
                     /locus_tag="PLD05_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78316.1"
                     /translation="MKTPLGQVDNPFSARFVRPGVIPYIFPPGCSWESVHEIFQANQR
                     LGAVIGPHGAGKSAFIAAFTHWMENNGQPCLALELHDGQRTLGQATWRKLEALQDTVV
                     TVDGYEQLSAWSRYQLRRLCRKKHLGLIVTAHTDIEIPLLFAPEPNEDLAWQIVQYLL
                     ARGQPLINRDDVRASFARHQGNLREMLFDLYDLYEVRRTSEVDSLGVVQASESSSEQE
                     HS"
     gene            2365..4827
                     /locus_tag="PLD05_12545"
     CDS             2365..4827
                     /locus_tag="PLD05_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOP78317.1"
                     /translation="MSTACRRRRGRFESLEPRYLLSGDYFWFTEGPLAVGTSPQSVAL
                     ADVNGDGILDLITAHDATSQVRVRLGNGDGTFGDAAIYGVGGQAYGLAVSDLNQDGSL
                     DILVSLRFEDRLAVLLGDGGGNFTLSDPITTGDYPTAIAVADFDADGRPDIVTADHNG
                     GTLSVILASSEGGFQPAVSYSSGNAPFAVKAGDINGDGILDLISVNSYSRTLAILLGR
                     GDGTFGSAITYSLGVFPYAVAVGDVNGDSALDVVVTNMGNDNVQVFLNNGSGELTAGA
                     VINTKDSPGPIALVDVNVDGRLDIAVACSNQDVVQVFLGAGDGSFTLWREANVGLYPT
                     DLAFGDINGDGRVDLVTANYDGNSVSILLQQLDTQPPISYFTSVPTEAWGLPVTLSWA
                     GDDQGGSGVASYNVWYRRVGETAYTLWLANTSTTSAAFQAEPGYSYELVVTASDIAGN
                     LEPLPDVPKATITLTVKTVDFLVISDVAAGSTLYPIKTSQGGVLTILAAGLNPSAGQR
                     VELYNASQQLVATSRTEGENERIDVTAAGPDEIYYVKISGENVFTTLTLVNLVAVDGQ
                     HVTVSGTPGVEWLVVTPETRELAINGVTYAWPTGDAIVIEAHGLGGNDWAQLYDSQGD
                     DVFTAKPGESRMEGPGYQIIVYDYFAVHGYAKTGSGNDIAHLFDSAGHEVVSGTSNFL
                     RVRGDSFILRAKFFGEVSISSTGGDDRLKLLGSEGNDTLSLTDDTIQFHTGNQIIKTN
                     IFSNIFISGGGGDDVATLDTGEDLVLLDATEERLRLLAPDVPYVLRIRGFESITARNQ
                     NPASTKKVASAVDYLMLEGTWA"
     gene            complement(4866..5495)
                     /locus_tag="PLD05_12550"
     CDS             complement(4866..5495)
                     /locus_tag="PLD05_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78318.1"
                     /translation="MGVLRGLGWVLLLLVVGCSRAATENRSDAGQQAGPTSLSQAEAV
                     PVAPSAEQPSPSSQADEEENMTATATEESSAGNNSTEEPLLLLEESDDGQDTHLMADN
                     SRCFVCHLNFQFEDIALVHAREGYGCAYCHGPSDAHIADESWASGGNGTAPDIMYRPN
                     EVIPACLKCHELSKSDPECHCDFPRLPQKKSCTDCHGNHRLKERTCHWK"
     gene            complement(5547..6575)
                     /locus_tag="PLD05_12555"
     CDS             complement(5547..6575)
                     /locus_tag="PLD05_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP78319.1"
                     /translation="MNTERQTRREFVQLTTWAVAGFAGGLRILSPQTAGATEPATPSP
                     TVDPSKILNYNENMEYRRLGKTELMVSAVCLGGHSRSDQQLRNEIISRCIDLGINYVD
                     ACWDNEVIRDAKALKGRRDKMYLALSHGAKEVRNEEYRTAKKLLESLDELLKASEQEY
                     TDLWRITCLEPGGRHSFDTSCEIVDALTKAKQQGKARFVGISSHDRRWLKFMIEYFPQ
                     IDVILFPFTTMSKAAPKDSLFPAIKQCDVGAFGIKPFGAGSLFSGNKAEDMRRARLAI
                     RYILATNTVVPIPGANSLEEVDNLALAVKERRSLDVKEQAELDQLNRHMWANLPRGYQ
                     WLKNWEYV"
     gene            complement(6609..7346)
                     /locus_tag="PLD05_12560"
     CDS             complement(6609..7346)
                     /locus_tag="PLD05_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOP78320.1"
                     /translation="MRSVRLLILMIGGCTLCLPNLYPVLGEEKPVQPLTGLVGWWPFD
                     ENGGNTAADLCPTPHPATLEGQAKFVAGRIGNAISLDGKGSRLRVPGFKGITGTSPRT
                     VSAWIKTSTASGQIVAWGSEEPGKMWIFGFIRSRIGVTPRGGYLYVKEPLHDGNWHHV
                     AVVLEEGSPPNLHDHARIFVDGELAEIHDIGLLDLWPIETGDTLDVTIGQGFNGLIDD
                     LRIYARVLTEEEIRSLFKAPETLKQSQ"
     gene            7571..8197
                     /locus_tag="PLD05_12565"
     CDS             7571..8197
                     /locus_tag="PLD05_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78321.1"
                     /translation="MAVRNLACMSLVLVAILGCSQSADHEAFRVKQSAVQPATPESKV
                     PPLASHISSEGEVTTQVGPPNLPPPERSGESFLSPPPMPQVEPGFTHSPVATPPMPSA
                     PIHQQAGTDVQVDLSAAVALPMTGPEGTMMGFSVDYRLKGVAGPPQSSYVWVIEGRPG
                     KVYRLPVKLDAQGNLMFIIQEWRPEDGPFRCRIEDYTGRIVSPVVDMR"
     gene            8243..8683
                     /locus_tag="PLD05_12570"
     CDS             8243..8683
                     /locus_tag="PLD05_12570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOP78322.1"
                     /translation="MLREHEIEIRVRYPECDPMGLLHHSRVFVYFEMGRTELLRASGG
                     NYRQLEEQGLFVVVVEARCRYYRPARYDDLLRLRTRVTRMSGYKIEHEYHLFRGEELL
                     CRGQVTLAVVDRTGQVRPIPEAWQLEFSKEAGESANLRSEVQSE"
     gene            complement(8696..>9510)
                     /locus_tag="PLD05_12575"
     CDS             complement(8696..>9510)
                     /locus_tag="PLD05_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78323.1"
                     /translation="RVAVTGSLKFDGTEFDRNNPRTQRLRDLARIRTDEIVFMAGSTQ
                     KEEELLALQVFQELRDAFPQLRLIIVPRHPERFDEVAKMLSQSGLPWQRRSQLIDPSE
                     TIRNRAQDPDHFYREVTPQQDHSVHSSTGFPRAPVLLVDTVGELTAWWGTAHIALVGG
                     SFGSRGGQNMLEPAGYGAAVCFGPNTWNFRDIVAHLLNAKAAVVVHNFEELRQFVQRC
                     LEYPDYAATLGENARKLILAHRGATQRTADCLQKLLGQSPASATPLRLKRSA"
BASE COUNT         2038 a         2534 c         2688 g         2250 t
ORIGIN      
        1 ccggtttcgt ctttagcgtt gggctttcgc ctcccaatgc cgctgccgct ttagcaacca
       61 gggacccatc ttccttaccg cttgcccgtt ctgggggcgc cgtatcgcgc gatttacgcc
      121 tggctggctg ctgcgctggg ctggaatatt gccgtcctcg ccttcgccct gggaacgtac
      181 tacgtgggtc gcttcgattg gagctttaca cttttcttat tgccgtgttt ggtgattggt
      241 cttaccttgc tcggtgcctt cttccggcag tggatggcca ccagcgccgt gggggaaacg
      301 attgtcgaga tttccgattt tccacttctc tgtggacatc gttaccgggt ttatttgaag
      361 cagacgggtt ggctgaggat caactggctg gaaatgttgc tcgtatgtga ggaggaggca
      421 acgttccagc atgggaccaa cacacgacga gagtgccgcc gcgtctggca aaagaccctc
      481 tggcggcacg agcggtttga aatcctgcgg agtgtccctt tcgagacgga ggcggaaatc
      541 gagattcccc atgggatgat gcattccttt cgttcgccac ataacgaagt ccgctggcgt
      601 ttgtatgtcc gcggcagtgc ccaggattgg ccgaactttg agcgggtctt tccgttggtg
      661 gtcctccccg ggccagagcg cgagcggaga ccggattccc ggccaggccg tgagggtctg
      721 tcttcggaaa ccccaagtga cgaagaatcc ccacaacagg tcaatccagg tcaggcctct
      781 gaaacatatt catgaccgag ccacggatac tgatccatct tgatggaaag aagactgttt
      841 acgaacccgg cgaagtactg gccggtcagg ttcggataga gcctttgccc cttcaggata
      901 tcaaagcggt ggagctgtcg attctctggt acacggatgg caagggagat caggaccttc
      961 acgtgcggta ttttcgccgc tggtcgcaag agggagattt accgcttgac ctgcgtgaac
     1021 ctctccgctt tcaaacggtt cttcctcaga caccgttaag ttacgacggt ttgatcattc
     1081 gaattcgctg gtgtgtgcgc gtgcgagtct ttcgcgcacg gggccgtgaa cttgtggaag
     1141 aggtcccctt ccggcttggc cgcgtagccc cggcccgggt gagattacaa tgaaaacacc
     1201 actgggacag gtggataatc ccttttcggc ccgcttcgtc cggccggggg ttattcccta
     1261 tatctttcca ccgggatgtt catgggagtc agttcacgaa atctttcagg ctaaccaacg
     1321 gctgggggcg gtgatcggcc cccatggtgc cggcaagtca gcctttattg cagccttcac
     1381 acattggatg gaaaacaatg gccaaccgtg tctggcgctc gagctccatg atggccaaag
     1441 aacactgggg caggcaacat ggcggaaatt ggaagcactt caggataccg tcgtgactgt
     1501 tgacggttat gagcaattaa gtgcctggtc tcgctatcag ttacgccgcc tctgccgaaa
     1561 gaagcatctc ggactgattg ttacggccca caccgatatt gagattcccc ttctctttgc
     1621 gcccgaacca aacgaggact tggcgtggca aattgtccaa tatctcctag cccgcggaca
     1681 accccttatt aatcgggacg atgttcgagc ctcgttcgcg cgtcatcaag gaaacttgcg
     1741 agaaatgctt tttgatcttt acgatttata cgaagtccga cggacgtcgg aggttgactc
     1801 ccttggtgtc gtccaggctt cagagtcctc gtcggagcaa gagcactctt gaagggaagc
     1861 ttgccgattt cttgtggcag ggcaagctgt tttcgatctt cgcctaagtc ttgcatgcgg
     1921 tcccggaaga ccggtaacga ttatcgagcc tgggacctgg atttcctatc cgtcaaatag
     1981 gaagtaaata aattgaagca catgtttttt ttctcgagat tcaagggatg tggttttttg
     2041 taacgtaaaa gttttcgcgg gattgctgaa gtctgtcccc cgctttggcg aggcgcgccc
     2101 ttgacttcta cggcattcca aaaatcctat agataggcga agggcaaatt agggaacttt
     2161 tctggtagtc acctccacct aatctgtaga cagtttctgt tcggacgaac ctggccggac
     2221 tgtgtgttac tgcgaaatcg gtatttcttt gaaggacagg gcagacgcgg gacgggatga
     2281 ttcgaaaaag ggacattcga tcggacgtca ggttgagttg gcctcttttt agatcgacga
     2341 ttgcagcgtt agaggtgaag cgacatgtcc acagcttgca gacgccggcg cggaagattt
     2401 gaaagccttg agccgcggta tcttctcagt ggcgattact tttggttcac agagggacca
     2461 cttgcggtgg gtacatctcc ccagagcgtg gcccttgccg atgttaacgg tgacggtatt
     2521 ctggacttga tcacggcgca tgatgcaacg tctcaagtca gggtccgttt gggcaacggg
     2581 gatgggacct ttggggacgc ggcgatctat ggtgtcggag gacaggccta cggactggcg
     2641 gtcagcgatc tcaatcaaga cggatcgcta gatattttgg tcagcctccg gtttgaggac
     2701 cgtttggccg ttctcttggg cgacggaggt ggtaacttta cgctgagtga tccaattaca
     2761 acaggtgact atcccacggc cattgctgta gccgactttg acgccgatgg ccgccccgac
     2821 atcgttacgg ccgatcataa cgggggcaca ctctctgtga tcctcgccag ctcggagggc
     2881 gggtttcagc cagccgtgtc ttattcatcg gggaatgcac cttttgccgt taaggccggg
     2941 gatatcaacg gcgacggaat cctggacttg atttccgtca attcttacag cagaactcta
     3001 gcgattctgc tcggtcgggg agatggcacg tttggaagtg ccattacata ttctctcggt
     3061 gttttcccat atgcggtggc ggtgggtgat gttaatggcg actccgcttt agacgtggtc
     3121 gtgaccaata tgggtaacga caacgtccaa gtctttctca ataatggttc gggagaacta
     3181 acggcggggg ccgtcataaa cacgaaggac tctccaggac ccatcgcttt agtcgatgta
     3241 aacgtggatg ggcgtctcga catagctgtg gcatgttcca atcaggatgt cgttcaggtt
     3301 tttttgggtg ccggggatgg ttcattcact ttgtggcgag aggccaacgt cggactctat
     3361 ccgacggatt tggcgtttgg agacatcaat ggtgacggac gcgtcgatct tgttacggcc
     3421 aactatgacg ggaactcggt ctctatcctc ttgcaacaat tggacacaca gcccccgatc
     3481 agctatttta cgtccgtccc cacggaggcc tggggccttc ccgtgacgtt atcctgggcg
     3541 ggggatgacc agggcgggtc tggtgtggcg agttacaacg tctggtatcg ccgggtcgga
     3601 gagacggctt ataccctgtg gttggcgaat acgtcgacga cgtcggcggc ttttcaggcg
     3661 gaaccgggct attcgtacga gctagtggtc acggcgagtg acatcgcggg gaatctcgag
     3721 ccgttgcccg atgtgcccaa agcaacaatt acgctgactg tcaaaaccgt cgattttctt
     3781 gtcatcagcg atgtagccgc tggtagtacc ctttacccaa tcaagacgag tcagggcgga
     3841 gttctcacga tcctggcggc aggcctcaat ccgtcggcgg gccagcgtgt tgagctttac
     3901 aacgccagcc aacagttggt ggcgacctcg aggacggagg gcgaaaacga acgaatcgat
     3961 gtaacggcag cgggacctga cgagatctac tacgtgaaga tttccggcga gaatgttttc
     4021 acgacattga cgttggtcaa cctcgtggcc gtcgatggcc agcacgtcac ggtctccggt
     4081 accccagggg tcgagtggtt ggtggtgaca cccgagacgc gggagttggc catcaatggc
     4141 gtcacttacg cttggccaac aggtgacgcc attgtgattg aggcccatgg tttaggcgga
     4201 aacgattggg cccagttgta cgattcgcaa ggagatgatg tattcacggc gaaacccggc
     4261 gaaagtcgga tggagggccc cggttatcag atcattgttt atgactattt tgcagttcac
     4321 ggatatgcca aaacaggctc ggggaatgac atagcccatt tgttcgattc agccggccat
     4381 gaggttgtca gcggaacgtc caacttcttg cgtgtcaggg gcgacagttt cattttgcgg
     4441 gccaaattct tcggggaggt ttcaatcagt tccactggtg gtgatgatcg acttaagctg
     4501 ctcggttccg agggaaacga taccctttct ttgacggacg acacgataca attccacacg
     4561 ggtaaccaga tcatcaagac gaacattttt tcgaatatct tcatctcagg cggtggaggt
     4621 gatgacgtgg ccaccctcga tacgggtgaa gacctggtat tgctggatgc cacggaagaa
     4681 cgactccggc ttctcgctcc cgacgtgcct tacgtcctcc gtattcgggg atttgagagc
     4741 atcacggccc gtaaccaaaa cccagcgagc accaagaagg tggcgtcagc cgtggattac
     4801 ctgatgctag aaggaacttg ggcttgacgc atataacaga ttgcccgaga agttagggcc
     4861 gagtcctatt tccagtgaca cgtccgttcc tttagccgat gattgccatg gcaatcggtg
     4921 cacgatttct tttgaggcaa tctgggaaaa tcgcagtggc attccgggtc tgacttggaa
     4981 agctcgtgac acttgaggca cgccgggatc acctcgtttg ggcggtacat gatgtcgggt
     5041 gccgtcccgt ttccacctga ggcccaagac tcgtcggcga tatgggcatc gctcgggcca
     5101 tgacagtatg cacaaccata gccttctctg gcatggacca atgcaatgtc ctcaaattgg
     5161 aagttaagat gacagacgaa acaacggctg ttatcggcca tcaaatgggt atcctgtcca
     5221 tcatctgatt cttccaacag aagcaagggt tcttccgttg aattgttacc ggcggaagac
     5281 tcttctgtgg ccgtggcggt catattctct tcttcatcgg cttggctgga cgggctgggt
     5341 tgctcggccg aaggcgctac cggaacggcc tccgcttggc taagcgacgt cggacctgcc
     5401 tgttggcctg catccgaacg attttccgtg gccgctcggg aacaaccgac cacaagtaac
     5461 aggagcaccc atccaagacc gcgcaaaaca cccatctgta gataccccaa agcggaacgg
     5521 cacccagaat acttcaccac acccgatcag acatactccc agtttttcag ccattggtag
     5581 ccgcgaggca agttcgccca catgtgacga ttcaactggt caagctcggc ttgctctttt
     5641 acgtccaagc tacggcgttc cttgacggcg agggcaagat tgtccacctc ttccaagcta
     5701 ttcgcaccgg gaataggaac cacggtattg gtggccagga tataccggat ggccaaacgt
     5761 gcccgtcgca tgtcctctgc cttattgccc gaaaagaggg acccggcgcc gaacggctta
     5821 attccaaagg ccccgacgtc gcattgtttg atggcgggga agaggctatc cttcggggcc
     5881 gccttgctca tggtggtgaa ggggaagagg atcacatcaa tctgcgggaa atattcaatc
     5941 atgaatttca gccatcggcg gtcgtgcgag gaaattccca cgaaccgggc ttttccctgc
     6001 tgcttggcct tggtcagggc gtccacaatc tcgcaggagg tgtcgaagct atgacgtcct
     6061 cctggttcca agcacgtaat ccgccaaagg tcggtgtatt cttgttccga cgccttgagg
     6121 agctcatcga gcgactcaag aagtttcttg gccgtacgat attcctcatt gcggacttct
     6181 ttggccccat gggagagggc aagatacatc ttgtcgcgac ggcctttcaa agccttggcg
     6241 tcgcgaatta cctcgttatc ccagcacgca tcaacatagt taattcccag gtcaatgcag
     6301 cgactaataa tctcgtttcg caattgttgg tcgctccggg aatgcccacc caaacagaca
     6361 gccgagacca tcaactccgt ttttcccaga cgacgatact ccatgttttc gttgtaattc
     6421 aaaatcttgg agggatcgac ggtaggggac ggagtcgccg gctcggtagc tcccgctgtc
     6481 tgcgggctga ggatgcgcaa tcccccagca aatcccgcta cagcccaggt cgtcagttga
     6541 acgaattccc gtcgtgtttg tctttcagtg ttcataatag cctcccattg cgtgcataaa
     6601 acctggcgtc attgtgattg tttcagtgtc tcgggagcct taaacaggct gcgtatctct
     6661 tcttcggtca atacacgggc ataaatgcga agatcatcaa tcaggccatt aaacccttga
     6721 ccgatggtta catccaaggt gtcgcccgtc tcaataggcc acagatccag caaaccgatg
     6781 tcatgaatct cagccaactc accatctacg aagattctgg cgtggtcatg aagattaggc
     6841 ggtgaccctt cttccaaaac cacagccaca tggtgccaat tcccgtcgtg gagaggctcc
     6901 ttcacataca gatagccacc ccgcggcgtg acaccgatcc gtgaccggat aaacccgaaa
     6961 atccacattt tgccaggttc ttcactcccc caagcgacaa tctgccctga agccgtactc
     7021 gttttgatcc acgcggagac cgttcgtggg gaggttcctg tgattccctt gaaacccgga
     7081 acacggagac ggcttccttt cccatcaagg gatatagcat taccaatgcg acctgcgaca
     7141 aatttggcct ggccctccag agtggcgggg tgtggcgtgg gacaaagatc ggccgctgta
     7201 ttgcctccgt tttcatcgaa aggccaccag ccgaccaacc ctgtcagcgg ttggacgggt
     7261 ttctcctctc ccaagacggg atacaaattc ggtaaacaaa gtgtgcatcc cccaatcatc
     7321 aaaatcaata gtcgcacact tcgcatatgt tcctccccag tgactccgat tgtgttttct
     7381 caagtctgcc ggcacgttgt ccattttggc ccctgcgaca tcggattgca agcgacgttg
     7441 cgcacccaaa gagcctcttg gcgggcaaat gattctaggt ttacactgat ctgaagacgc
     7501 aattgcttcg gcgaagctgg gtttgttggc gtgtcctggc atgccgacat ggccacaagg
     7561 agacgcagtc atggcggtac gcaatctagc ctgtatgtcg ctcgtgctag tggcaattct
     7621 gggctgttcg caatcggcag atcatgaagc ctttcgtgta aaacaatcgg ccgttcaacc
     7681 ggcgacaccc gaatcaaagg taccgccctt ggcgagtcac atttcgtccg agggagaggt
     7741 aacgacccag gtggggccgc cgaacctgcc tcctccggag cgatccggtg aaagctttct
     7801 ctcgcctcct ccgatgccgc aggtcgagcc tggtttcaca cattcgccgg ttgcgacgcc
     7861 accgatgccc tcggcgccaa ttcaccaaca agctggaacc gacgtccagg tggatctctc
     7921 agcggccgtg gcccttccta tgactgggcc ggaggggaca atgatgggct tcagtgtgga
     7981 ttaccgtctg aaaggcgtag cgggccctcc gcaaagttcc tacgtttggg tcatcgaagg
     8041 taggccggga aaggtctatc gattaccggt gaaactggat gcacaaggca acctcatgtt
     8101 tatcatccaa gagtggcgac cggaagacgg accattccgt tgccggatcg aggattacac
     8161 gggtcggatt gtttcgccgg tggttgacat gcggtaggcc cggcatcttg ggttggcccc
     8221 acaggaatgg caggaggatt tcatgctacg agaacacgag attgaaattc gtgttcgcta
     8281 tcccgaatgc gacccgatgg gactgttgca tcacagccgg gtctttgtct atttcgagat
     8341 gggccgaacg gagttactac gggcatcagg cggcaattac cgccagttag aagaacaggg
     8401 actatttgtg gttgtagtcg aggcacgatg tcgctactat cgtccggcac gttatgacga
     8461 tctgttgcga ttgagaacac gcgtgactcg catgagcggc tataagatcg agcacgagta
     8521 ccatcttttc cgcggtgagg aactcctctg ccgaggccaa gtgacgttgg ccgtcgttga
     8581 tcgaacgggc caggtccggc ccattcccga ggcctggcaa ctcgaattct cgaaagaggc
     8641 tggagagtct gccaatctcc gttcagaggt tcaaagcgaa taatttattg acccgtcaag
     8701 ctgatcgttt caatcgcaaa ggggtggctg aagccgggct ctggccaaga agcttttgaa
     8761 ggcaatcggc ggttctttga gtggcaccgc ggtgcgcaag gattaacttc ctcgcattct
     8821 ccccgagtgt ggcggcgtag tccggatatt ccaagcatct ctggacaaat tggcggagtt
     8881 cctcaaagtt gtggacgacc acggccgcct tggcgttgag caagtgggcc acaatgtcgc
     8941 ggaaattcca cgtgttagga ccaaagcaaa cagcggcgcc ataacctgcg ggttccagca
     9001 tgttttgccc accacgagag ccgaagcttc caccaaccag ggcgatgtgg gccgttcccc
     9061 accaggctgt aagctctccc accgtatcga caagaagaac cggtgcccgg gggaaaccgg
     9121 ttgacgagtg gactgaatga tcctgctgtg gcgtgacttc gcggtaaaaa tggtctgggt
     9181 cttgtgcgcg atttcggata gtctcgctcg ggtctatcag ttgtgaccga cgttgccagg
     9241 gtaggccgct ttggctaagc atcttagcaa cttcatcaaa acgttcagga tgccttggca
     9301 cgataatcag ccgcaactgg ggaaaagcgt cacgcaactc ctgaaagacc tgaagtgcca
     9361 ggagttcctc ctctttttga gtgctacccg ccataaacac aatttcatcg gtccgaatcc
     9421 tggccaggtc gcggagccgc tgtgttcgtg ggttgttacg gtcgaattcc gtgccatcga
     9481 atttcaagga tccggtgact gcgacgcggt
//
LOCUS       DAOSUT010000153         9441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3917_length_9441_cov_9.928297, whole genome shotgun sequence.
ACCESSION   DAOSUT010000153 DAOSUT010000000
VERSION     DAOSUT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_9441_cov_9.928297"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PLD05_12580"
     CDS             complement(<1..1295)
                     /locus_tag="PLD05_12580"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOP78324.1"
                     /translation="MGETAKLILGGQEYELPIRIGTEGNHVIDISKLYQMTGHITLDE
                     GFANTGGTTSSITYIDGELGILRYRGYRIEELAERCDFLEVAYLLIYGELPTAEQLEE
                     FRDNIRQHTLLHEGMKQIFAGFPPDAHPMAILSSVVGALPIYYKDSLDPLDENQRQLS
                     IYRLLAKMPTIAAFGYKQSIGQPFVYPQNHLTYCQNFLYMLFAVPSERYRMDPDFVDA
                     LNLLLILHADHGQNCSTSAVRMVGSSNVNLFSAIAAGICALWGQLHGGANEACITMLE
                     QILADNRNIGKYIDKAKDPNDSFRLMGFGHRIYKNYDPRARIIKQMADRLIAKRMGTD
                     PLFELAKELEEIALKDPYFIERKLYPNVDFYSGIIYRAMGIPKNMFTVLFALGRLPGW
                     IAHWREMHLTPNKRIHRPRQLYIGYPERPFVPLMKRSPKP"
     gene            complement(1677..3170)
                     /locus_tag="PLD05_12585"
     CDS             complement(1677..3170)
                     /locus_tag="PLD05_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78325.1"
                     /translation="MIDQSSRIQPLSLLIFAVTLMFTATLGLAWPNFAQEYQVLGHLV
                     GRTGPGEDYPVAAELRGSTRASKVAEEGNGWVALRLDDGRVVWVTAEDLRSDAALLAS
                     IPPLRPPLTAGPTAAETNGKKPVQTGAPLSSTGGDQIPQTGGPSAAPQSVIDANWRPV
                     SAGASKMGVSPPTAFESKTLPQTQQVSRADPPTSPAAQNPDDLLDQAELELSTILTKD
                     PTQWRFDSIQDKVASLAASRLTPAQIARMDALAAKLATARQIADLSLKAHAYSQPMPG
                     PVVLPYSAAGQPPGSPVRHPTGNLGTSPPGTFSTNALTVVPPPSTRITPESRMAGTRG
                     NTVAIATNQPSVFPPSTPSDLGGIGTTAVFPASFEAPSTGELPQSATPSGPAEMSPEA
                     APGTSRPGPGLSLREEIARELNELRRERFDAVGRLVKAQVRRPSDPPYLVVDESGAIV
                     CYVKPAPGTLLRTYVGHWVGISGHKARLPDGRGLLITAESLRLLDPP"
     gene            complement(3341..4339)
                     /locus_tag="PLD05_12590"
     CDS             complement(3341..4339)
                     /locus_tag="PLD05_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOP78326.1"
                     /translation="MGPLLFEPYFRPQIWGGRRLANVLGKLLPPNGRYGESWEVSAHP
                     LHVSRLRGQSSSESTLASLWEKSKMSLWGESYPSPVEFPWLVKFLDCDDFLSVQVHPD
                     SQLAAELVPTERGKDEVWVIVSADAGAKVYLGLRPDATPSRLRQAIQRGHPEDCLKVY
                     SPQPGDIFHIPPGTVHSAGGGVLIAEIQTSSDATFRLYDWNRLDERGQPRPLHIEQAF
                     RAIHWQREVRAPLRTEFQPRGPGWLSQLAASTANFHIECWQIAARTQIEPPHLGMTVA
                     MVLSGSVILRVNSSPPLTLPKGETVLIPACVNSVLWSNPLDLPAWLVVCSPPVQPR"
     gene            complement(4676..5686)
                     /locus_tag="PLD05_12595"
     CDS             complement(4676..5686)
                     /locus_tag="PLD05_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOP78327.1"
                     /translation="MRPRIVLCFPNEERHRRKIAETAPWADVVDVPQERIAEELLNAD
                     IFCGHAKVPVDWDAVVRRGRLRWIQSSAAGMDHCLVPSVIESEIIVTSASGLLADQVA
                     EHTVALVTAWLRSLPVFLRAQEKQEFIRRPTRDLHRSTILIVGFGGVGRRLAQILHTF
                     KTTLWAVDLFPVDCPPYVKQLVPPENLLSLLPGADIVILSLPLNDQTRGMFNRQLFAQ
                     MKRGSLLVNVARGPIVVTDHLIEAIQSGHLAGAVMDVTDPEPLPPGHPLWTTANVIIT
                     PHVGGQSATRADDVVRIMCENLRRWHEGRPLVNLITDKRLGFPKRDGSTPLWIDICDQ
                     IP"
     gene            6106..6813
                     /locus_tag="PLD05_12600"
     CDS             6106..6813
                     /locus_tag="PLD05_12600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOP78328.1"
                     /translation="MRRREFLITSSVVGTNVVLGSQLVKSMENLVTVEGGQRMAFTQP
                     PLPYPMDALRPFLSEEQVTFHYGKHHAAYFRNLNNLVEGKPEAQLSLRELVIQAPAGP
                     VFNNAAQAWNHSFYWDCLSPKGGGEPADALGAAIARDFGSFAEFRAAMSDAAVKLFGS
                     GWAWLAGDKDGKLKILALSNAETPLKLGLEPVLTIDVWEHAYYIDYRHERAKYVEAFW
                     GYVNWEFVAKCYAAATG"
     gene            7562..8551
                     /locus_tag="PLD05_12605"
     CDS             7562..8551
                     /locus_tag="PLD05_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOP78329.1"
                     /translation="MVLLRITIGWHFLYEGVWKIANRDTFSAEPFLTQAKGPFAPIFF
                     AMVPDLDGKARLQVVKDKDGRVRVVSPPYEIAWERACDDFIRYYNMDDKQSKAARETC
                     ERFVTGLQNYLAENQEAILGYLASLKRFEEEEKASPNNGAHFQRERLWERQQKLRAEV
                     AGWLRQIDAMGEAYQRALWDLLTEEQKAKGMLKVPLCETQRLPVRIPWIATRSELLNV
                     VVTYSLTAIGLCLILGFFTRLANLGGALFLAFVVMTQWPWPTVVPKTPEIVGHALFVD
                     KNFVEMMAMLALAALPVGRWAGLDYFLYHYVCVPLRQRWGCCCCSKAAEQKKG"
     gene            8631..>9441
                     /locus_tag="PLD05_12610"
     CDS             8631..>9441
                     /locus_tag="PLD05_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78330.1"
                     /translation="MNLTPEEREIGKANFHRALGNNLEVTRRDFLQSGLLTALAAGGS
                     VGAFYFGYESTIEKPLRVAVLGTGDEGQVLIGACNPEFIDIVAIADIRPFNIHRAFHG
                     DWSSDAALEARPGLMKVYGWKTEDEARKHVKVYGHYEELLKEEKDNIEAVIIALPLHL
                     HAPAAVAAMKLGKHVLTEKLMGHSVHECKEMARIAKKAGLHLAVGHQRHYNILYENAS
                     DMIKRGLLGDLHYIRAQWHRGNLPGRDSWAMPLPPGAKPDDTSHKTLLSQLK"
BASE COUNT         2118 a         2400 c         2810 g         2113 t
ORIGIN      
        1 ggctttggcg atcgcttcat gagcgggaca aatggccgct cgggatagcc gatgtacagt
       61 tgccgcggac ggtggatgcg cttgttcggg gtcaaatgca tttcccgcca gtgggcaatc
      121 cagccgggca aacgccccag ggcaaaaagg acagtgaaca tattctttgg aatgcccatt
      181 gcccgataaa tgatgcccga ataaaagtcc acgttggggt acaatttacg ttcgatgaag
      241 tacggatcct tgagcgcgat ttcctctaac tccttggcaa gctcgaacag gggatccgtt
      301 cccattcgtt ttgcgatcag gcggtcggcc atttgcttga tgatcctggc ccgggggtca
      361 tagttcttgt agattcgatg gccaaacccc atgaggcgga agctgtcatt cgggtctttg
      421 gccttatcaa tatactttcc gatatttcga ttgtcggcca agatctgctc cagcatggtg
      481 atgcaggctt cgttcgctcc tccgtggagc tgaccccata gggcacaaat acctgccgca
      541 atggccgaga agaggttcac gttgctggaa ccgaccatcc gtacggccga ggtactacag
      601 ttctggccgt ggtcggcatg cagaattaac agtaagttaa gggcatcgac aaaatccgga
      661 tccatgcggt accgttcgct gggaacggca aaaagcatgt acaggaagtt ttgacaatac
      721 gtaaggtggt tttgggggta cacgaaaggc tggcctatgg attgcttgta accgaaggca
      781 gcaattgtcg gcatcttggc cagcagccgg tagatagaaa gttggcgctg gttttcatcg
      841 agcggatcaa gtgaatcctt gtaataaatc ggcaaggcac ccaccaccga gctaaggatt
      901 gccatgggat gggcgtccgg gggaaaaccg gcaaagattt gtttcatacc ctcgtgaagg
      961 agggtatgct gccgaatatt gtcccggaat tcttccaact gctcagccgt cggcagttcg
     1021 ccgtaaatta gtaagtacgc gacttcgaga aagtcgcacc gctcggccag ctcctcaatt
     1081 cggtatcctc ggtagcggag gataccgagt tcgccgtcga tataagtgat gctgctcgtt
     1141 gttcccccag tattagcgaa gccttcatcc aacgtgatgt ggcccgtcat ctggtagagt
     1201 ttcgagatat caataacgtg atttccctcc gttccaatgc gaatgggaag ctcgtactct
     1261 tgtccgccga gaatcagctt tgcagtctcg cccattgttt ctccaagtgt ggcgcaacaa
     1321 ataggttcag gctacaggta ccaccattaa cgtatgtccc aaaacctctt attctactcg
     1381 ctatgatgca acggaaaaac tgaggggttc ggcaatggct tccgctatta cagactcacg
     1441 caggcccaaa acgccagaaa tcggccattt tgccgattct ttccctatgt tggtggaccc
     1501 gagataccgc ggcaggtcgt gatcactttc atcatccccg tagctcgaga gcgttaaatg
     1561 accagccgtc ggacatgcgg aaagacaagt ggctgacaca attaaccgaa gagcggggaa
     1621 aaacgcactc gatttggaca gccgggatcg tacccttcct gttggattct acctccctac
     1681 ggtggatcaa ggagacgaag ggactcggct gtaatgagta gtccacgacc atcgggcaaa
     1741 cgggccttgt gtccgcttat cccgacccaa tggccgacgt atgtgcgcaa aagcgttccc
     1801 ggggctggct taacgtagca aacgatggct cccgattcat caactaccag atatggtggg
     1861 tcgcttggtc gccggacttg ggccttaacc aatcgtccca cggcatcgaa acgttccctg
     1921 cggagctcgt tgagctcgcg agcaatctct tctcgaagcg ataggccggg gcccggacgg
     1981 gatgtgcccg gggctgcttc tggtgacatc tccgcgggac ccgagggggt ggcggactgc
     2041 ggtaattcac cagtggaagg cgcttcaaac gaggcgggaa acaccgcggt ggtacctatc
     2101 cctcccaagt cgctgggcgt agaaggtggg aatacggagg gctgatttgt agcaatagcc
     2161 acggtattac cccgggtgcc cgccattcgg ctttccggcg tgattctggt ggatggtggg
     2221 gggacaaccg tcaatgcgtt agtggagaac gttccagggg gtgacgttcc gaggttacct
     2281 gtcggatgcc gcacgggtga gcctgggggt tggccggcgg ctgagtacgg tagtacaaca
     2341 ggccccggca tgggctggct gtaggcgtga gccttgagtg ataagtccgc aatttgcctg
     2401 gctgtggcca atttggccgc tagggcatcc attcgcgcga tttgggccgg ggtcaaacga
     2461 cttgccgcaa gcgaagccac cttgtcttgg atggaatcaa aacgccattg ggttgggtct
     2521 ttcgtcaaga tggttgacaa ctcaagttcc gcctgatcca aaaggtcatc tgggttttga
     2581 gcggctggcg acgttggtgg atcagcacgg ctgacctgct gggtttgtgg caaagtcttt
     2641 gattcgaagg ccgttggggg ggaaactccc attttagaag ccccggcact gacgggtcgc
     2701 caatttgcgt caatgaccga ttgcggcgct gccgaaggtc cccctgtttg aggtatctgg
     2761 tcaccaccgg tcgatgagag aggggccccg gtttggacgg gcttcttgcc gttggtttcc
     2821 gcagcggtgg gtcccgccgt taggggcggg cgcaggggtg ggatggatgc caaaagtgcc
     2881 gcgtcgctac ggagatcttc tgcggtgacc cacacaacac gtccgtcgtc aaggcggagg
     2941 gcgacccaac cgttaccctc ttcggccacc ttgctcgccc gggttgaccc ccgcaactcc
     3001 gcggcaaccg ggtaatcttc accggggcca gttcgaccaa caaggtggcc aaggacctga
     3061 tactcctgag caaagttggg ccaggccagt cccaaagttg cggtgaacat caacgtcact
     3121 gcaaagatca gcagtgataa tggctgtatc cgactggatt gatcgatcac gagtgccact
     3181 cctcttggat aaggcacgct ttctcaaaaa gcaacatttg cgcggagcac cgcgggcggg
     3241 tattcccaca tcggataagc cttatagcgt cccaactcag ggtaggcaag gtcaaaagag
     3301 tgggtcaacc acctccaagt gaagacgctg gccgcttacc tcatcggggt tgtaccggag
     3361 gagaacacac gacgagccat gccggtaggt ccagcgggtt ggaccataga accgagttga
     3421 cgcaagcagg gatgaggact gtttcaccct ttggcagagt gagcgggggt gagctattca
     3481 cgcggagtat gacggagcct gagagtacca tggcgaccgt cattcccaag tgggggggct
     3541 caatctgtgt cctcgccgcg atttgccaac attcaatgtg gaaattggcg gtgcttgcag
     3601 caagctggga aagccaccct ggtccacggg gctggaattc cgtacgcagc ggggcacgca
     3661 cctctcgctg ccaatggatg gcacgaaaag cctgctcaat gtgcaatggt cggggttggc
     3721 cacgttcatc gagccgattc cagtcataaa gccgaaaagt ggcgtcacta gaggtttgga
     3781 tttcagcgat gagaaccccc ccgcctgcgg aatggactgt ccccggggga atgtgaaaga
     3841 tgtcacccgg ttgcggtgaa tagactttca gacaatcttc cggatgaccg cgctggatgg
     3901 cttggcgcag gcgtgacggt gtcgcatcgg ggcgaagacc cagatagact ttggcccccg
     3961 catcagccga gacgataacc catacttcat ctttgccgcg ttcggtagga actaactccg
     4021 ctgcgagttg gctatcgggg tggacctgga cagaaaggaa atcatcacag tcgagaaact
     4081 tgaccaacca ggggaactcc acgggactgg ggtatgattc cccccagaga gacattttag
     4141 atttttccca gagtgatgcg agagttgatt cggagctgct ctgaccccgc aatcggctga
     4201 cgtgaagagg gtgggcactc acctcccagg attcaccgta acgtccgttc ggggggagca
     4261 gtttacccaa aacgttcgca agccggcgcc caccccagat ttgtggacga aaatatggct
     4321 cgaaaaggag tggtcccatc ccgatgattc ccaccaaatt gagcttgctt ttccagcttt
     4381 ggtcgtcggg aaaagcagcc ccatcttccc acaaaacgcg aactggttct accagtttcc
     4441 ctaggcctga ggcacttgtc aaaacttgtc tttgccgctg cctgcgatga ctgggcaagt
     4501 tgccgaagcc tacctagagc ctatcccaaa aaagtcccct ctccctccgg gggagggcta
     4561 aagtgagggt aagcggccgt cggagtggat cgagaaaacc ggctgcgatt cgagccaccc
     4621 tcacctgcga cccctctccc gctgaacggg agagggaaga ttatgggatg ggcttttagg
     4681 gaatttggtc gcaaatgtca atccacaaag gcgttgaacc atcgcgcttg ggaaatccca
     4741 agcgtttatc cgtgatcaag ttgacgaggg ggcgcccctc gtgccagcgc cggagatttt
     4801 cgcacatgat acggacgaca tcatcggccc gcgtggcgga ctgcccccca acatggggcg
     4861 tgataatgac gttggcggtt gtccaaagtg ggtggcctgg ggggagtggt tcggggtcag
     4921 tcacatccat aacggcacct gccaggtgtc cgctttggat cgcctcaatc agatgatcag
     4981 tcacgacaat ggggccccgg gccacgttta caagcagact accccgtttc atctgcgcga
     5041 aaagctgacg attgaacata ccgcgggttt ggtcattgag cggtagtgaa aggatgacaa
     5101 tgtcggcgcc gggcagcaag gaaagaaggt tttcaggagg aacgagctgt tttacgtagg
     5161 ggggacaatc cacgggaaaa agatcaacgg cccagagtgt cgttttgaag gtgtgaagaa
     5221 tctgggccaa tcgcctgccc acgccgccaa agccgacaat aagaatggtg ctgcgatgca
     5281 agtctcgggt aggcctgcga ataaactcct gtttttcctg tgcgcggaga aacacgggca
     5341 aacttcgcaa ccaagctgta acgagcgcca cggtgtgctc cgccacctgg tcggcaagga
     5401 gcccggaggc gctggtgacg atgatctctg attcgatcac ggaggggacc aggcaatgat
     5461 ccatccccgc tgccgaagac tgaatccagc gtagtcgccc acggcgaacg actgcgtccc
     5521 aatcgaccgg gactttagca tggccacaaa agatgtcggc attaagcaat tcctcggcga
     5581 tgcgttcctg aggcacatca acgacgtcgg cccagggcgc cgtctcggcg atcttacggc
     5641 gatgtctctc ttcgttggga aagcacaaga caatccgtgg acgcatgaag caaaatctcc
     5701 caagcggtaa gtgtcttcat tggagcccca gataggatta ccggccaata tttcgaagac
     5761 tccacagcgc caccacagtc atgctgcccc aaccaacgtt agtgcacgac agctctgagt
     5821 gttcgcggcg gcttgttatg aagtcgttgt tgctcaaccg gtaagatagg cctaatctcg
     5881 tctcctgggg agggggtgtt cggggcggcc taaaacggcg atctagcttt caattcaatt
     5941 gcttgcatgg cgtaaaccaa ggtgttatat tgaccgccaa aaagatacgc ggtgcctgca
     6001 atgtcgatgg tgcgccctta tcttcgaaaa gtggcgatgt atggtacgga aagggagggt
     6061 ttgagaaact cgtcttgaat gcgcctttca catgggaggg aaatcatgag acgtcgcgag
     6121 tttttgatca cttcatcggt ggttggtaca aacgtggtcc ttgggtcgca actggtcaaa
     6181 agtatggaaa atctggtcac agtagaagga ggacaaagga tggcattcac gcagcccccg
     6241 ttgccttatc caatggatgc tcttagaccg tttttgtccg aagagcaagt cacgtttcac
     6301 tatggcaagc accacgccgc gtacttcagg aacctcaaca acctggtgga agggaaacct
     6361 gaagcccagt tatcgcttcg tgaattggtc atccaagcgc cagcagggcc ggtgttcaac
     6421 aatgctgccc aagcctggaa ccacagtttc tactgggatt gcctttcgcc caagggcggt
     6481 ggcgagccgg ctgacgcgct tggtgctgcc atcgctcggg actttggaag ttttgccgag
     6541 tttcgcgccg ctatgtctga cgcagcggtg aaattgttcg gctcggggtg ggcttggttg
     6601 gcgggcgaca aggacggcaa gttgaagatc ctggccctca gcaacgccga gactcccctg
     6661 aagttgggcc ttgagccagt cttgaccatc gacgtgtggg agcacgctta ctacatcgat
     6721 tatcgccatg agcgggcgaa atatgtagag gcgttttggg gctatgtcaa ctgggaattt
     6781 gtggcgaagt gttatgctgc cgcgacaggc tgattttgtg acggaggtcg aacttcgggc
     6841 cttacgttgg cctgactttc gaggctactg ataatcaccc cgtgttagct ggtctttttg
     6901 tccaaatttg cggcgccagt ccgactaggg gaggttgcct ttttggacaa cctccccttt
     6961 atgcgttcgt cgcgatagct agccgagacg tggagctacc ggctgcggaa atgcgacggg
     7021 ccaactcagg ttaacttccg gaagaacgtc tgatgatttt gtattggctc tggtccttgt
     7081 gaatcttcgg tcccgttcat ttttcttcgg aaagtgcatc ggcggtatcc cgcggtgagt
     7141 ttcattaggc gtaccaccga cggaacaaag accgatcttg ttgcgtagtt gaaagtagcc
     7201 acacttcaga ggttgaccgg ccagttgtgg caaatagcca ccagaaggtg ctcccaaaag
     7261 gatctggcga tgtgggccga cgggaattgg tccgcgccga ggaagggacg aaaaaacggc
     7321 ccgagcctgg aacatttttg gccccgtttg gttctggcaa acacccgccg ttggaattaa
     7381 aatgacccta acaggtcttg aatagttggc ccactcccga agtgttgcgt ttgtggtact
     7441 gtttaccgag cccaccgggg atgcattttt ggtcttgatc ggggtttgac cgatcgcata
     7501 cggaaggagg ttagccctga accgctcagc ttttacgatt ggcctttcgg cagttgtact
     7561 attggttttg ttacgtatca cgatcggttg gcattttctt tatgaagggg tttggaaaat
     7621 tgccaatcgg gatacattct ctgcggagcc ttttctaacc caggccaaag ggcccttcgc
     7681 ccctattttt ttcgcaatgg tgcccgattt ggatgggaag gcacgcctcc aagtagtcaa
     7741 ggacaaagat ggccgagtcc gggtcgtatc tcccccgtat gagattgcat gggagcgggc
     7801 gtgtgacgat tttattcgtt attacaatat ggacgacaaa caatcgaagg cagcgcggga
     7861 aacgtgcgag cgtttcgtga cgggcctgca gaattatctg gcggaaaatc aagaagccat
     7921 tttgggctat ttagcaagct tgaaacgatt tgaggaagaa gagaaggcct caccgaataa
     7981 cggtgcccat tttcaacgag agcggttatg ggaacgtcag caaaaactgc gtgctgaggt
     8041 ggccggttgg ctgagacaga tcgacgcgat gggagaagct taccagcgcg ctttgtggga
     8101 tttgctgacg gaggaacaaa aagcgaaggg gatgttaaag gttcccttgt gtgaaacgca
     8161 acgtctgccc gttcgcatcc catggattgc aacgagatcg gaactgttga atgtggtggt
     8221 tacttactcg ctgacagcga tcggcttgtg ccttatcctg ggattcttca ctcggttggc
     8281 aaatttgggg ggcgcgttgt ttttggcctt tgtcgtgatg acgcagtggc cttggcccac
     8341 ggtcgtccca aagactcccg aaatcgtggg ccatgcgctc tttgtggata agaattttgt
     8401 ggagatgatg gccatgctgg cccttgcagc actgcccgta ggacgatggg ctggtttaga
     8461 ttatttcctg tatcattacg tatgtgttcc gctgcggcag cgctgggggt gctgttgctg
     8521 cagcaaggcg gcggaacaga aaaaggggtg atcgcgaatt gttggtcatt ggcgtctgaa
     8581 agatgtggaa gcgataaacg attggctatc agcgataaag gatcgtcacg atgaacttga
     8641 cccctgaaga acgtgaaatt ggtaaggcga actttcatcg ggcattggga aacaatttag
     8701 aggtcacccg gcgggatttc ctccagtcag ggctcctaac cgccctggcc gcgggtggaa
     8761 gtgtgggagc gttttatttc ggctatgaaa gcacgattga gaagccgttg cgggtagctg
     8821 tgcttggaac tggggacgaa ggtcaggtcc ttattggggc ctgtaacccg gagtttatcg
     8881 acattgtggc cattgcggac attcgtccct ttaacattca tcgggctttc cacggtgact
     8941 ggtccagtga tgcggccctg gaagcgaggc ccggcctgat gaaggtgtac ggatggaaaa
     9001 cggaggacga ggcgagaaag catgtcaagg tctatggcca ttatgaagaa cttctaaagg
     9061 aggagaagga taacatcgag gcggtcatta ttgcattgcc gcttcacctc cacgcacctg
     9121 ctgcagtggc tgcgatgaaa cttggcaagc acgtgctcac cgaaaagctg atgggccatt
     9181 cggtccatga atgcaaggaa atggcgagga tcgccaagaa ggccgggttg catttagccg
     9241 tgggacatca gcggcattac aacatccttt atgagaacgc cagtgatatg atcaaacgcg
     9301 gcctactcgg cgatctccat tacatccgtg ctcagtggca tcggggaaac ctgccgggac
     9361 gagatagctg ggcgatgccc ttgccaccag gggcgaagcc cgacgacacc agccacaaga
     9421 ctcttctctc acaacttaag a
//
LOCUS       DAOSUT010000154         9276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3982_length_9276_cov_9.094241, whole genome shotgun sequence.
ACCESSION   DAOSUT010000154 DAOSUT010000000
VERSION     DAOSUT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_9276_cov_9.094241"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLD05_12615"
     CDS             complement(<1..220)
                     /locus_tag="PLD05_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78331.1"
                     /translation="MHHPSPRSDRWSHCAGPNRRAFLRTVLKGASAITGVTTLLSWEK
                     WAVAVEPSTADRFSLRYLLASCLYGYADL"
     gene            complement(248..1510)
                     /locus_tag="PLD05_12620"
     CDS             complement(248..1510)
                     /locus_tag="PLD05_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78332.1"
                     /translation="MMLTSVGVPHLWIRRVQAGEGTQSPNERVGLGAIGMRYQGTVIT
                     LIAQNHGNVVAIADVDRNVREQARASFGSTPAIYEDYRDLLDRKDVDVVLIGTPDHWH
                     AKMVIDACRAGKDVYVEKPLTLTIDEGKVIRRVVEETKRVVQVGSWQRSDARYRLAVE
                     LARAGRLGKLQRVTCVAGPNPTGGPFPVRPVPAALNWNLWLGQAPEVPYTEERCHYTF
                     RWWFEYAGGKITDWGAHDIDIAQWAIGELPVEVKGHGKLPNIPNGYNVPIDFGAVVRY
                     PSGVELIVQEEGRVGVLFEGEKGRLFVNRGTISGSVVDELKDHPFPKDQYKLYDFDDP
                     NRPDRVGKLDAIVNHMANFFDCIRARRLPISDYESQHRSATTCHLVNLAIRLGRPLKW
                     DAEKEIFVGDDEANQHLSRPQRKGFEVA"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1623..3465)
                     /locus_tag="PLD05_12625"
     CDS             complement(<1623..3465)
                     /locus_tag="PLD05_12625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOP78333.1"
                     /translation="MMADALRIGVYVCHCGTNIAGVLDTADLARFASGLPHVHVARDY
                     KYMCSEPGQELIRQDIREYGLNRIVVAACSPHLHEATFRKVLQEAGLNPYLLAIANIR
                     EHVAWITADRESGQKRARAQIAAAVGRVARQRPLEPSSYPVTPRVLVVGGGIAGIQAA
                     LTLADAGKEVILVEREPTIGGHMAKFDKTFPTLDCAACILTPKMSAVKEHPRIRLLTY
                     SEVESVQGSAGRFKVKVRRRPRYIREDLCVGCLQCIEACVFKALKIPNEFDLGLAKRK
                     PIYLPFPQAVPPVPVIDPQTCLRLRTGKCKQTCVEACADRQAIDFEQQEWIEEYEVGA
                     IIIATGFRTFDPTPLAYYGYGRFPEVYTALEVERLLNASGPSGGEIVLRDGATPRVVG
                     IVHCVGSRDHNYHPYCSRVCCMYALKLAHLIRERCGAEVYNFYIDIRAPGKAFEEFYE
                     RTQKEGVHFVRGKVGEVERLSTNGDGLRLMVTVEDSLLGRVRKIPVDMLILAVALEPA
                     EGAEQVRQLFHISCSSEGFFLEKHPKLAPVNTASDGVFIAGACQGPKDIPDTVAQADA
                     AAAQALALIDRGVVEAEPNIAWVDENVCSGCRVCVSLCPFDAITPDEE"
     gene            complement(3531..5789)
                     /locus_tag="PLD05_12630"
     CDS             complement(3531..5789)
                     /locus_tag="PLD05_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOP78334.1"
                     /translation="MVPSRPTFWNIPAWSEILQYLLGAGVVVFIVWVVIRHIRCWRKG
                     TREPVPRLGAEVLKQALERLARFVLTPQRLQSDPYAFITHLGLFWGMVLLALGTAIAT
                     IDWDVFHLIFGVRLLQGAFYRVFELVLDVAGVALLIGLVLAVVRRYVLRPSRLTVPQF
                     FGESWESAVILGLLLLVTITGFLIEGLRIQAGLMLKSAAVIANTPENMGEEGVRDATA
                     AAAASVAPWSPVGNLVATIFRGLDASSLRQAHLVLWWIHALAAFAFLLAIPVTKANHL
                     ITAFLNVILPSSSELPTASPTIKQRIDGVLPSLTWRQRLEVSACTACGKCQEVCPAHR
                     AGYAFTPKGTVWATRGVLMREVWPWWPATAKASEEHVWLNKDAIWSCYTCRACEVQCP
                     VLVRHTNLVVSYRRPLVDESALADGLQEVLQNLQRYGNSFGQSPRKRADWTKGLPFAV
                     KNANKEAVHYLWFVGDYASYDARAREVTQTIARIFNAAGLDYGILYEKEKNAGNDVRR
                     IGEEGLFEFLRDENLRALESATWQAIVTSDPHSYHVLRNEYGLKKPSPVGPPASTEKP
                     HEAVGKNSNTVASQALADPPGGNGHPAKHNGDAAPLVWHVSELFWELLKSGKLTVAKK
                     LDRRVTYHDPCYLGRYNEVYDAPRAVLEAIGCHVVEMPQHRENSYCCGAGGGRIWMKD
                     RPGIRERPAETRVKEALSLGNVEDLVVACPKDLVMFQDAVKSLGAEDRLRVIDVASLL
                     AQAISPDIKSQD"
     gene            complement(5790..6767)
                     /locus_tag="PLD05_12635"
     CDS             complement(5790..6767)
                     /locus_tag="PLD05_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOP78335.1"
                     /translation="MIRRIVVPVEVYRGHITEATREALVAARTVGNGVGEVVAYVYGE
                     PPAEATDVLELADRIIVLEDGQVGTPVAMAQAKLLRERIASSDVDLVLVGSSSWGWDV
                     APLLAGFLSAPLVANCQVIQHAEDVLKATCRICAGKLLVDVEITNKPTVILLLPGSYA
                     HREVQPGKGVMERERVPASDWDARVEFKGWLEPEREDIDITQEEILVAVGRGIGQQEN
                     LELVEDLAKTLGGAVCGSRPVVDQGWLPPTRQVGKSGMIVKPKLYLAIGISGAPEHVE
                     GMKDAELIVAINRDEQAPIFDVAHYGIAADLFDVIPALKEALVARRQEA"
     gene            complement(6764..7537)
                     /locus_tag="PLD05_12640"
     CDS             complement(6764..7537)
                     /locus_tag="PLD05_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78336.1"
                     /translation="MNILAVVRLVPDLVEPLVVTAEEDNLNWDQTAFTLNESDDHAVE
                     EAILLKEKYGGHVSVVAFDFGDIDQVLYMLAAKGVDRVIKISWSHDEPPNSLEVARTL
                     SRLVPKLNVDLVVIGCQSHTEFLGMAGPQLAVALNWPYVGVVRGVQEGGTAGRWTAFK
                     EYPGAVLAQMEVSLPAVLGILTASQPPRYVPVSRIRAAAQSVRFETADTPAEIEEPPV
                     KIQRVYLPPAVTHAEMLTGSEAEVAQRLVDILLARGVLK"
     gene            complement(7534..8664)
                     /locus_tag="PLD05_12645"
     CDS             complement(7534..8664)
                     /locus_tag="PLD05_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOP78337.1"
                     /translation="MQCGLCTSSCPLGDAMEYPPRRIIAEAWNNSLERVIHSPSVWMC
                     VGCYTCSLRCPREIDLTDTVWPAVRDYALQEGIQPPPELRTAFQNLYLYGNVFGYSPR
                     KRLDWARNLEVTVRDLSKSPEPVEVLWLVGCYPSYHPRNQVVARDMARILTALGVRWG
                     ILGKEERALGDCDRLFGEEGLFEMLVENNRRLFERFKFEKIVLLDPHAYRALEKFYPR
                     FGASFPAEHYTTFLASRLDQIRPWLKKPINARVTYHDNCCVGRRCLCFDPPRQLLQAI
                     PGVELVEMKRNREHALCCGGGGGGMWLDAFITEHGGRRLSDVRIQHALETGADILAVS
                     CPFELSRFEDAVKVARAEDRLKVRDIIELLADSMGLGEGGGR"
BASE COUNT         2175 a         2746 c         2282 g         1973 t
ORIGIN      
        1 cgagatcggc atatccataa agacaggagg ccaaaagata tcgcaacgaa aaccggtcgg
       61 cagtcgatgg ctccaccgcg actgcccatt tttcccagga caaaagcgtc gtgacgccgg
      121 taatggcgga tgcacccttc aaaaccgtcc gcagaaacgc gcgtcggttt ggccctgcac
      181 aatgtgacca cctgtcactt cggggcgacg gatgatgcat aatccctccc tttcagacga
      241 aagcatgtca ggcgacttcg aatcctttgc gttgaggccg agataagtgc tgattggctt
      301 cgtcgtctcc gacgaaaatt tccttttcgg catcccactt caggggtcgg ccaagtcgaa
      361 tggctaaatt gacgagatgg cacgtcgtcg cgctacgatg ttggctttca taatccgaga
      421 taggaagccg acgcgcccgg atgcaatcga aaaagttcgc catgtggttc acaatcgcat
      481 ccagttttcc cacgcggtcc ggacggttag gatcatcgaa gtcataaagc ttgtattggt
      541 cttttgggaa aggatgatcc tttagctcat caacaacact ccctgaaatt gttccgcgat
      601 taacaaatag ccgtcccttt tctccctcga acaaaacgcc cacgcggcct tcttcctgaa
      661 cgatcagctc aactccactt gggtagcgga caacggcgcc gaaatctatc ggaacgttgt
      721 aaccattcgg gatattcggc agtttgccgt gccctttgac ctccacggga agctcgccaa
      781 tcgcccattg cgcgatgtcg atatcatggg ccccccagtc cgtaatcttg ccgccggcat
      841 attcaaacca ccagcggaag gtgtaatggc agcgttcctc ggtgtagggg acttctggag
      901 cttggccaag ccacagattc caattgagcg cggccggcac gggccgaaca gggaacggtc
      961 ctcccgtagg attaggccca gccacacacg tcacccgctg gagtttgccc aatcgacctg
     1021 cccgtgccag ttccacagcg agtcgatatc gcgcatcact tcgctgccag gagccaacct
     1081 ggaccacccg ttttgtttct tcgacgactc ggcgaatgac ttttccttcg tcaatggtca
     1141 gggttaacgg tttctcgaca tacacatctt tgccggcccg acatgcgtca ataaccatct
     1201 tggcgtgcca gtggtcgggt gtaccgataa gaacaacatc aacatctttt ctgtccaaaa
     1261 ggtcccggta atcctcatag atagccggtg tactgccaaa actggcccgt gcttgctcac
     1321 gcacgtttcg atcgacatcg gcaatggcaa caacattgcc gtgattctgg gcaattaacg
     1381 tgatgaccgt cccttgatat cgcattccga tcgcaccaag gccaactcgc tcattcggcg
     1441 attgcgtacc ttcaccggcc tgcactctcc gaatccacaa atgaggaaca cctaccgagg
     1501 tgagcatcat cccagtgatg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nntttcttcg tcaggcgtaa ttgcatcaaa tgggcaaagc gaaacgcaaa cacggcagcc
     1681 agagcagacg ttctcgtcca cccaggcgat attcggttcg gcctcgacaa caccccgatc
     1741 aataagcgcg agggcctggg ccgctgccgc atcagcctgg gccaccgtgt ccggaatatc
     1801 cttcggccct tggcaggcgc ctgcgatgaa cacgccatcg ctagcggtgt taaccggagc
     1861 caacttagga tgcttttcga gaaagaaacc ttcagaggag cacgatatat ggaagagctg
     1921 cctcacttgc tcagcgccct cggcaggttc cagggcgaca gccaaaatca acatgtccac
     1981 aggaatcttg cggacacgac ccaataaact gtcttccacc gtgaccatca agcgaagtcc
     2041 gtctccattt gtggaaagcc gctccacctc gccaaccttg ccccgcacaa aatgaacacc
     2101 ctccttctgg gtccgttcgt aaaactcctc gaaggcctta ccgggagcac ggatatcaat
     2161 gtagaagttg taaacctcgg ctccgcatcg ttcgcgaatc aagtgggcca acttcaacgc
     2221 gtacatacag cagacgcgag aacaataggg atggtaattg tggtcgcggc tgccgacgca
     2281 gtgaacgatt ccaaccactc gcggtgtcgc accatcccgt aagacaattt ctccccccga
     2341 cggtccggaa gcattcagca agcgttccac ctccagcgcg gtgtacactt ccggaaagcg
     2401 tccatagcca taatacgcga gaggcgtagg atcgaatgtt cggaagcctg tggcaataat
     2461 aatggcccca acctcgtact cttcgatcca ttcttgttgt tcaaagtcaa tcgcctgacg
     2521 gtccgcacaa gcctcgacgc acgtttgctt gcacttgccc gtccgaagac gcaaacatgt
     2581 ttgcgggtca atgacgggta cgggcggcac agcctgcgga aacggcagat aaatcggctt
     2641 acgtttagcc aggccgagat caaattcatt cggaatttta agggccttga acacgcaggc
     2701 ttcgatacat tgcagacacc ccacgcaaag gtcttcccga atgtaacggg gccgccggcg
     2761 gaccttgacc ttaaaccgcc ctgctgaacc ttgaacggat tccacttccg aataggtcaa
     2821 tagtcggatt cgcggatgct ccttaacagc ggacattttc ggagtcaaaa tgcaagccgc
     2881 acaatccaag gtggggaatg tcttgtcgaa cttggccatg tggccgccaa tggttggctc
     2941 gcgctcgacg aggatgacct cttttccagc gtcggccaga gtcaacgcgg cctggatccc
     3001 agcgattcca cctccgacca caaggaccct gggcgttacg gggtaagagc tgggctcgag
     3061 tggtctttga cgggcaaccc gtcccacggc tgcggcaatc tgagcgcgtg cccgcttttg
     3121 gccgctttcc ctgtccgcag taatccatgc cacatgctca cggatgttcg caatggccag
     3181 gaggtaagga tttaagccgg cttcctgaag aacctttcga aatgtggcct cgtgcaaatg
     3241 aggcgaacaa gcagccacca cgatgcgatt caggccgtat tccctaatgt cctggcggat
     3301 gagttcctgg ccaggttcag aacacatgta tttgtaatcc cgggcaacgt gcacatgggg
     3361 caagcctgac gcaaagcgag caaggtcggc ggtatccagg accccggcaa tgttggtacc
     3421 acaatggcac acgtaaacac caatgcgcag cgcatccgcc atcatgatcc cacctccgct
     3481 atcagtttta tttcagatgg cttgtaattc aaaaagccaa gatgaaagtt tcaatcttga
     3541 cttttaatgt caggagaaat ggcttgcgct agcaaactgg ccacgtcaat cacccggagt
     3601 cgatcctctg cgcctagaga tttcaccgca tcttgaaaca tgaccaggtc ttttggacat
     3661 gccacgacca ggtcctccac gttgcccaga gaaagcgctt ccttgaccct ggtctcggcc
     3721 ggtcgctccc gaattccagg acgatccttc atccagatgc gcccaccacc tgcaccacaa
     3781 cagtagctat tctctcggtg ctgcggcatt tccactacat ggcaaccgat tgcctcaaga
     3841 acagcgcggg gggcatcgta aacctcgtta tagcgcccca aataacaagg atcatgataa
     3901 gtcactcgac ggtctagctt tttggcgacg gtgagttttc cggatttcag taattcccaa
     3961 aaaagctcac taacgtgcca taccagcggc gcagcatcgc cattgtgctt cgccgggtga
     4021 ccattcccac ccggtggatc agcaagcgct tgggaagcta ccgtattaga attctttcca
     4081 acagcttcgt ggggcttttc agtagaagca ggaggtccta cgggagacgg tttttttaat
     4141 ccgtattcat ttcgcaagac atgataagaa tggggatcag acgtcacgat tgcttgccat
     4201 gtggcgcttt caagggcacg aagattttca tctcgcaaaa attcaaaaag tccttcctca
     4261 cctatccgcc ggacatcatt tccagcgttt ttctcctttt cataaaggat gccgtaatcc
     4321 agacccgccg cattaaaaat gcgtgcgatt gtctgggtaa cttctctggc tcgggcgtca
     4381 taggaagcgt aatcacctac aaaccacaag taatgaaccg cttctttgtt cgcgttttta
     4441 accgcaaagg gcaaaccttt agtccaatca gcacgtttgc ggggtgactg cccgaatgag
     4501 tttccataac gttgaaggtt ttgaaggact tcttgaaggc cgtcggcaag ggcgctttca
     4561 tccacaaggg ggcgtcgata tgaaacaacc agattggtgt gccgcacaag gacaggacat
     4621 tgtacctcgc atgcccgaca ggtataacag ctccaaatgg cgtccttatt gagccagacg
     4681 tgctcttcgg aggccttcgc ggtggcaggc caccatggcc agacctccct catcagtact
     4741 ccgcgcgtcg cccagaccgt gcctttcggc gtaaaggcgt atccggctcg atgtgccggg
     4801 cagacttcct ggcatttacc gcacgctgtg caggcgctga cctcaagacg ctgccgccac
     4861 gtcaaggaag gaagcacccc gtcgattctc tgcttgatcg tcggtgatgc cgttggcagt
     4921 tctgatgaac tcggtaggat cacgttgaga aaagcggtga tgagatgatt tgccttcgtt
     4981 accggaattg ccaagagaaa tgcaaaagcc gcaagggcat gaatccacca cagcaccaga
     5041 tgcgcttgtc gcagcgatga ggcgtccagc ccgcgaaaga tcgtcgcgac taagttaccc
     5101 accggtgacc acggtgccac gcttgccgct gctgcggccg tcgcatctcg caccccctct
     5161 tcgcccatgt tctctggagt gttggcgatc accgccgccg atttcagcat gagtcccgcc
     5221 tggattcgca gtccctctat tagaaatcct gtgatcgtca ccagaaggag caagcccaaa
     5281 attaccgctg actcccagct ttccccaaag aattgaggaa cagtcaagcg agacggccga
     5341 agcacgtaac gtcgcaccac agctaacaca aggcctatca gcagggctac gccagcaacg
     5401 tctaaaacta attcaaacac gcgatagaag gcgccctgca aaagccgtac tccgaagatg
     5461 aggtggaaca catcccagtc aatggtggcg attgccgtcc ccaaagccag aagaaccatt
     5521 ccccaaaaca gccccaggtg agttataaac gcataaggat cggattgaag ccgctgcggc
     5581 gtcagaacga agcgagcaag acgctccagc gcttgtttga gaacctcggc gcccaatcgt
     5641 ggaactggtt ctctggtacc ctttctccag cagcgtatat gccgtatgac tacccaaacg
     5701 atgaacacga ccacccctgc tcccaacaaa tactggagga tttcactcca ggccgggatg
     5761 ttccagaagg tgggtcgcga tggcaccatc taagcctcct gtcgtcgcgc aacaagggcc
     5821 tctttgagag caggaataac gtcaaagaga tctgcagcaa tcccataatg ggcaacatcg
     5881 aaaatcgggg cctgctcatc acggttaata gccacaataa gttccgcgtc cttcattccc
     5941 tcaacgtgtt caggtgcccc gctgatacca attgccaaat agagcttcgg ttttacgatc
     6001 atgccggact tacccacctg ccgcgtgggt ggaagccagc cttgatcgac caccggtcgg
     6061 gacccacaca ccgcaccacc caaggttttg gccaaatcct ccaccaactc caggttctcc
     6121 tgttgcccga taccgcggcc aactgcaacg aggatctctt cctgggtgat atcaatgtcc
     6181 tcgcgttccg gttctaacca tcctttgaac tccacgcgcg cgtcccaatc actcgccggg
     6241 acgcgttcac gctccatgac gccctttccc ggctggactt ctcggtgcgc ataacttccg
     6301 ggcaatagca ggatgactgt cggtttattt gtgatttcca cgtccaccag caacttgccg
     6361 gcgcaaatgc gacaggtagc tttgaggacg tcttctgcat gctgtatcac ctgacaattg
     6421 gccaccaaag gtgccgaaag aaagcccgcc aagaggggcg caacgtccca tccccaacta
     6481 ctgcttccca ccaaaaccag gtccacgtcg gatgaggcaa ttctttcgcg aagcaacttg
     6541 gcctgagcca tggccaccgg cgtcccaacc tggccatctt ccaacacgat gatacggtcg
     6601 gccagctcga ggacatctgt ggcctcggca ggtggttctc cataaacata agcgacgacc
     6661 tctccgacgc cgttgcccac cgtgcgagcc gcgaccaacg cctcgcgagt tgcctcggtt
     6721 atgtgcccac gatacacttc aaccggtaca acgattcgac ggatcatttg agcactcctc
     6781 gggccaagag aatatcgact aaccgttgtg cgacctcagc ttccgagcca gtcagcattt
     6841 cggcatgggt gaccgcgggc ggtaggtaca ccctctgaat ctttaccggt ggctcttcaa
     6901 tttccgccgg agtatcggcg gtctcgaatc tgacactctg agcggcagca cggatgcgac
     6961 ttaccggcac ataccggggc ggctggctgg ccgtcaaaat ccccagtacc gcgggaagcg
     7021 aaacttccat ctgagccagc acagcccctg ggtactcctt aaacgctgtc catcgcccag
     7081 ccgtgccgcc ttcctgcaca ccgcgtacaa cacctacgta aggccaattc agtgctacag
     7141 ctagttgagg cccagccatc cccagaaact cggtgtggga ttggcatcct atgaccacga
     7201 gatcgacatt gagcttgggg accaagcgac tcagggtcct tgccacttcc agactgttcg
     7261 gcggctcgtc atgcgaccaa gaaatcttga tgaccctgtc cacacctttc gcagccaaca
     7321 tgtagaggac ctggtcaata tccccaaaat caaacgccac aaccgagaca tgtcctccat
     7381 atttctcttt cagcagaatt gcttcttcca cagcgtggtc atcgctttcg ttgagagtga
     7441 acgccgtttg gtcccaattc aagttgtcct cctccgctgt caccacaagc ggctcaacaa
     7501 ggtcaggtac caaacgtaca acagctagga tattcatctt ccacctcctt cgcccaaccc
     7561 catcgagtcg gccaaaagct cgataatgtc gcgtacctta agacgatcct ctgcacgcgc
     7621 caccttgacg gcatcttcaa aacgggatag ttcaaaagga caggaaacgg ccaatatgtc
     7681 ggcacccgtt tcaagggcgt gttggatgcg cacgtccgac aaccgccgcc caccatgttc
     7741 ggtgataaag gcatccaacc acattccgcc gccgcctccg ccacaacaga gtgcgtgctc
     7801 gcggttcctt ttcatttcta ccaactctac accggggatt gcctgcaaaa gctggcgtgg
     7861 gggatcgaag cagaggcacc ttctacccac gcagcaattg tcgtggtatg tcacccgtgc
     7921 attgattggt tttttcaacc atggacggat ctgatccaat cgagatgcca gaaacgtcgt
     7981 gtaatgctcg gcaggaaacg atgccccaaa tcggggataa aacttctcca aagcacggta
     8041 ggcgtgggga tcaagaagca cgatcttttc aaatttgaac cgctcgaata atcggcggtt
     8101 gttttccacc agcatctcga acagaccctc ctcgccaaac aaccggtcac agtcgcccag
     8161 tgctcgctcc tctttgccaa ggatacccca acggacaccc agcgccgtaa gaatccgcgc
     8221 catatcgcgc gccactacct gattgcgggg atgatacgaa gggtaacatc ccactagcca
     8281 tagcacctcg acgggttccg gcgactttga caaatctcga acggtaacct cgaggttccg
     8341 agcccaatcc agccgcttcc gaggagaata gccaaagacg ttaccatata gatacaagtt
     8401 ttggaaagcc gtccgtaact caggtggcgg ctgaattcct tcctgtaatg cgtagtcgcg
     8461 aacagccggc caaactgtgt ccgtcaggtc gatttcgcgt gggcatcgta acgaacacgt
     8521 ataacaccct acgcacatcc agacggaagg actgtggata actcgttcga gcgagttgtt
     8581 ccacgcctcc gcgataatcc gccgcggcgg atactccata gcgtcaccca gcggacaaga
     8641 actggtgcac aaaccgcact gcatacaagc tcatgcggtt gagccttcca aaggtgagaa
     8701 aagcatctga tagaactcag ataccctcga gccagatgag gtcgccggag gaacttgcat
     8761 agccgtacct cctgcttaaa gtagagccct cccgacgcgc cctgtggacc gatcgtcctt
     8821 cgcaagacat tcaggcgcat tatcggcacc cattttgttg ccccgaagac tgttgtcaat
     8881 aacctatttg ggggatgtac aaaagatacc cgtaaatggt gccgttgccg ccacattgtg
     8941 cagtttcctt gatcttggca tgtggtgtgt atgtgaatac ccattctcgt cttgcgcttg
     9001 acccacggaa acttctcacg gttgtagcct gcgaggccac gagaattgtc acacggccca
     9061 ccgtgttctc aaaggacaag caggcatgga gagcaatagg ctgcgctccc agtcaagctc
     9121 aacttttgcc atttttcggg tgccgttttt ggggagaatt gatcttggca tgggagccga
     9181 attcgcgcaa aaaagactcc ttcctggtta tgttaactga caccttgccc agaaaggagt
     9241 ctagaatgtt gccgttgcct tccgtggcgg agccgc
//
LOCUS       DAOSUT010000155         9272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3983_length_9272_cov_9.386026, whole genome shotgun sequence.
ACCESSION   DAOSUT010000155 DAOSUT010000000
VERSION     DAOSUT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3983_length_9272_cov_9.386026"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /gene="polX"
                     /locus_tag="PLD05_12650"
     CDS             <1..1731
                     /gene="polX"
                     /locus_tag="PLD05_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOP78338.1"
                     /translation="ITVTNAEIAAVFEQIADLLEFQGANPFRVRAYRTAARNIGELTE
                     PLAEIVAAGDRDLTELPGIGQDLAEKIVTLLKTGSLPLLEVLKSQVPESVLTLLRVPG
                     LGPKRAAALYKKLQITTLEELRQACLDHRVRELEGFGSKTEENILKGIEAAIQAGQRI
                     LWYEADQIVQSLREHLEPTAGLHRLVPAGSYRRGKDTVGDLDFLALADTPDPVMDRLA
                     TFRGVKDILARGDTKMSMVLQNGIQVDLRVVPAESFGAALQYFTGSKEHNVILRGMAK
                     NRGLKINEYGVFRGEEQIAGREEEEVYAALDLPWFPPELREGRKEFEWAAAGKLPDLI
                     TLDDIRGDLHIHSTWSDGMATIEEIAEAARARGYQYIAITDHSQRVTVANGLNAERLL
                     AQWEAIDRLAKKWRGFHILKGVEVDILEAGGLDLPDEVLKEADWVVASVHFGQNQPRD
                     QITRRMIDALKNPYVCAIAHPTGRLLVERPPYEVDIDAVLRAARDYGKMMELNAHPRR
                     LDLDDVACAVAKAYGVPIAISTDAHSVSGLDAMRYGVMQARRGGLTRADVVNTRPWRE
                     LKKLLPKYRK"
     gene            complement(2174..3175)
                     /locus_tag="PLD05_12655"
     CDS             complement(2174..3175)
                     /locus_tag="PLD05_12655"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOP78339.1"
                     /translation="MDRRTFLKLSGAVAAQAALWSPQVGRAEAESASAHGLGNPFLQE
                     GRWFKAAFHVHTTTSDGDVDVPTRLNQYRKLGYQVVAITDHWKTNDLSAFAEEDFLPI
                     VGLEMHPQTGTGAPAHHFVVLNVPHPFELDRKLAAQAMIDQVLSVGGKVIYAHPYWTT
                     HSLNEMLEVAGYCALEVFNGGCHVENDTGYGSVHWDQVLNKGRLIGGIATDDVHNSKL
                     INIGWTMIKAPALNAAAIMEAIEKGCYYASAGPTIEDCRIEDGVIRVITSPVRRITFR
                     CNGVGGGHVVQAKPGETLTSAEWKFAGSRRRYQWIRVEVADEMDNRAWTNPFVIPTA"
     gene            4385..7066
                     /locus_tag="PLD05_12660"
     CDS             4385..7066
                     /locus_tag="PLD05_12660"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOP78340.1"
                     /translation="MNVPLRSRWSSARRWLTLGVALLVGLLSYHLVFAEEATSAAPAS
                     PSLGGQLSVFVWWIIGLGGSICGLYFAWRFFKEMLNSEKGNERMEEIASYVREGANAY
                     LKQQYTVVACFFVVIVILLAIAAFGLHAQSRWVPFAFLTGGFFSGLAGWLGMKTATLA
                     SSRTAQGCRKSLNDGLRVAFRSGAVMGLTVVGLGLLDICLWFAILYWVISAFGYHMTL
                     EQITVTMLCFGMGASSQALFARVGGGIFTKAADVGADLVGKVEKGIPEDDPRNPAVIA
                     DNVGDNVGDVAGMGADLYESYCGSILATAALGVAAFATGELLPTRILEQVQKGAIALE
                     SAKLNYQLAALFLPIVLAGVGIALSVIGIYAVRSEEDATQRTLLKALGRGINLSTLLI
                     AVATFVFTYLLMPDFWTVAVSVLVGLIAGWLIGKWTEYSTSDEYQPTKFIASQSQTGP
                     ATVIIGGFAEGMKSTWFPVIVVAVATLLAFGFAAQFHFNDLTWFAMGLYGVGIAAVGM
                     LSTLGITLATDAYGPIADNAGGNAEMSHLEPQVRQRTDALDSLGNTTAATGKGFAIGS
                     AALTALALLAAYVEEVRVGFDRWAHSALEQAEPGLYKLGDKFLAEVTGEKGPGGKPII
                     HGYLVFGSAITDPAVAEDWNKLDASREPQKIPEGIIERFEDEVKGIKRTRLQFAKTKA
                     PLIRVDRATMPDFANYYEAHLMNPKVLVGVLIGAMLPFLFSSLTMKAVGRAAYRMVEE
                     VRRQFREKPGIMEGTEKPDYATPVRISTQAAQKEMILPSLLGLLTPVAVALILGVTGV
                     MGLLMGALASGFCIAVFMANAGGAWDNAKKHIERGAYGGKGTDAHKAAVVGDTVGDPF
                     KDTSGPSLNILIKLMSMVSVVVAGLAVRYSLASLGWF"
     gene            complement(7183..7746)
                     /locus_tag="PLD05_12665"
     CDS             complement(7183..7746)
                     /locus_tag="PLD05_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP78341.1"
                     /translation="MNLTPRFDEALQFAVSLHRDQRRKVNDAPYIGHLLRVCGLVLEY
                     GGTEDEAIAALLHDSLEDQNRPGLAEEIAARFGETVLSIVQACSDTVVQPKPPWRERK
                     EAFLERLRTANAAVRLIQAADKLDNVSILLGQYRVFGPEIWNKFKGGREGTLWYYRRV
                     AEVLGWASENPLVLELARRVEELEQLA"
     gene            complement(7774..8733)
                     /locus_tag="PLD05_12670"
     CDS             complement(7774..8733)
                     /locus_tag="PLD05_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78342.1"
                     /translation="MTRFYSLLLLIGGIAVAWLVIPQVYTLSKGNLPIREIRPGSSPS
                     GETPAEPIAILPPAKKQRAFRIVVWDVTPLDQRKVLEPGVLTAIATIVHQFDLAVLQG
                     VDLRDRSVLAQLLDHIDPKNETFHYVTVPEPLRLVTRRGTVVLFDMRALEVDRRTVQV
                     VDDPQRRLQVPPLVAAFRARGVPPEKAFTFSLVSYSVDPFRADEELSLLDEVFRAVRD
                     DGRGEDDVIVVGNFSRDYRVITAALRVPDGTCAIMGLPSNVSGTESSQNIVFDGRATI
                     EYTGRRGVVDLLRQFQLTTAQAEQVAQHLPVWAEFSVYEGGAP"
BASE COUNT         1932 a         2563 c         2748 g         2029 t
ORIGIN      
        1 atcaccgtga ccaacgctga aattgcggct gtctttgagc aaattgcgga cctgctggag
       61 tttcaggggg ccaatccctt tcgggtaagg gcctatcgca cggccgcgcg gaatatcggg
      121 gaactgacag aaccattggc cgagattgtc gccgccggcg atagggacct cacggaattg
      181 cccggcattg ggcaagactt ggcagaaaaa attgtcacgc tcttaaagac cggctctctt
      241 cctctgcttg aggtgctaaa gtcccaggtg cccgaaagcg tcttgacgct gcttcgcgtc
      301 cctggtttgg gtcccaagcg tgcggctgcc ctctataaaa agctccaaat cacgacactg
      361 gaagagctac ggcaagcctg tctcgatcac cgtgttcggg agttagaagg attcggaagt
      421 aagaccgagg agaacatcct taaggggatc gaggcggcca ttcaggcggg acagcgaatc
      481 ctttggtacg aagccgacca aattgtccaa tcgctgcggg agcaccttga gccaacagcc
      541 ggcctgcatc ggctggtgcc agcaggaagc tatcggcgag gtaaggacac cgtgggcgac
      601 ctggatttcc ttgctctggc cgatacgccc gacccggtga tggatcgctt ggccacattt
      661 cgtggggtga aggacattct tgcccgcggc gatactaaga tgtcgatggt cctgcaaaac
      721 ggtattcagg tggacctgcg ggtagtgccg gccgagtcgt tcggggccgc cctgcaatac
      781 tttaccggtt cgaaggagca taatgtcatc cttcgcggaa tggcgaaaaa tcgtgggctt
      841 aagatcaacg aatatggggt gtttcgcggg gaagaacaga ttgccggacg ggaagaagag
      901 gaggtctatg ccgctttaga cctgccctgg ttcccgccgg aattgcggga aggtcgcaaa
      961 gaattcgaat gggctgcggc aggcaaactc cctgatctaa tcaccctgga cgacatccga
     1021 ggggacctcc acatacacag tacttggtcg gatggtatgg ccaccattga agaaatcgcc
     1081 gaagccgccc gcgcccgagg ctatcagtac attgccatca ccgatcactc ccaacgggtc
     1141 acggtggcca acggtctgaa cgcggagcga cttttggcgc aatgggaggc catcgatcgg
     1201 ctggccaaga agtggcgggg gtttcacatc ttgaaaggtg tggaagttga catcctagag
     1261 gccggtggac tggatttgcc cgacgaggtc ctcaaggaag ccgattgggt agtggccagt
     1321 gtgcactttg gacagaatca accccgcgac cagatcacac ggcggatgat cgatgccctt
     1381 aagaaccctt atgtctgcgc cattgcccat cctacgggcc gtcttctcgt tgaaagacct
     1441 ccctatgagg tggacatcga tgcggtactc cgagcagccc gcgattatgg aaagatgatg
     1501 gaactgaatg cgcatccacg ccgtttggat ctcgatgatg tggcctgcgc cgtggccaaa
     1561 gcctacggcg tgcccatcgc catctcgacg gatgcccaca gtgtcagcgg tctggatgcc
     1621 atgcggtacg gtgtcatgca ggcccggcgg ggtggtttga cgcgggctga tgtcgtcaac
     1681 acgcgaccct ggcgcgaatt gaaaaagctc ctgccgaaat accgcaaata agcatctcta
     1741 ccagcctggg attcttttcc tcacaccctc gtcttagggc ggaattgtat tgcgtggatg
     1801 agccagcggg cttctaccca cagtgaggga gtaccttcga gcaagtaggc gcgcaaacat
     1861 ttacagccta tcccaaaaag tctccctctc cctccgggag agggttgggg tgagggcaag
     1921 cgaacctcgc aatcgattga gaaaaccggc atcgcttcga gccaccctaa cccccgaccc
     1981 gtctcccgcc caaagggaaa ggggagatta tgggatagac tcttaatcgt ccagcggcgt
     2041 ttctatactt ttctcttgcc caataccgga ccagtcaagc cggtgccaaa tggccgtgct
     2101 cgaagtaact gagcgcgcgg tctcttactc ttaaggggac cactctcgcg gccactatcc
     2161 cgtggaatgt tctttacgcg gtggggatga cgaaaggatt cgtccaggcg cggttgtcca
     2221 tttcgtctgc cacttccacg cggatccact gataccggcg gcgcgaaccg gcgaatttcc
     2281 actcggctga cgttaaggtt tcgcccggct tggcctggac gacatgccca ccgcccaccc
     2341 cgttgcaacg gaaggtaatc cgtcgcaccg gactggtgat cacgcggatg acgccgtcct
     2401 cgatccggca gtcctcaatg gtcggtcccg ccgaggcata gtaacagcct ttttcgatcg
     2461 cttccatgat ggctgctgca ttgagtgcag gggccttgat cattgtccag ccaatgttga
     2521 tcaatttgga attgtggaca tcatccgtgg caatcccacc gatgagccgc cctttgttca
     2581 aaacttggtc ccaatggacg gaaccatagc ctgtgtcgtt ttccacgtgg caaccaccat
     2641 tgaagacttc taaggcacag taaccggcta cctctagcat ttcgttcaac gagtgagttg
     2701 tccaataggg gtgggcatag atgaccttgc cacccacgga aagcacctgg tcgatcatcg
     2761 cctgcgcagc gagcttgcga tccagctcga acgggtgcgg cacgttgaga accacaaaat
     2821 gatgcgccgg ggcacccgtt cccgtttgcg ggtgcatttc caaaccgacg atggggagga
     2881 aatcttcctc agcaaatgca ctgaggtcgt tcgttttcca gtgatccgtg atcgcaacca
     2941 cctggtaacc cagcttacga tattgattca gccgcgttgg aacgtccaca tcgccatcgg
     3001 aggtcgtcgt gtggacatgg aaggctgcct tgaaccagcg tccctcttgg agaaatggat
     3061 tgcccagccc atgtgccgac gccgattctg cctcggctcg acccacttga ggggaccaca
     3121 aagcggcctg ggccgccacc gctccggaca atttgagaaa ggttcgtcga tccacgatgc
     3181 taactcctta tcagtgttga gggcgtaaac gagttccgaa aaaccacgtt ggcatattcc
     3241 agaatataag gcagcgagat taagacgtgc aagtagcaga cagaatctct catggtcacg
     3301 gaagacacct tgagcccaac ccgtgacgga gacgacgagc cgcaaggaaa gaatcatcat
     3361 gagcactaag caagaagcaa gaaatgatgg caagccccgc tgtgagctgg gatgtgtgtt
     3421 gatgatctat gcgtgtttca ctgattcgta gaggcaggtc ttgtctctgt ccgcctggga
     3481 cacccctcac gcgtcgtgga tgaaggccgg gggccgccgt agccgggctc ggcaatggat
     3541 catcaatcag agtctatccc aaattccctc tccctccggg agagggttgg ggtgagggta
     3601 agcaaccccc ggagtccatc gagaaaacca gcagcgattc gatccacgct caccctctgg
     3661 tccttctccc gcggaacggg aggggggatc ttgggggata ggtttttagc aggacgtcag
     3721 acgtgatggc gtccctccct cgaaatgcgg gaggcatggg atgcagtctt cggtattaag
     3781 gaaatcccca tcgtttggtg gctgttgaga aagcgagtcc cgccgattca gtcgttttta
     3841 tcagaaagaa ttgatatttc attcaggtaa attaggggat gtgtgtcaat aggtggctac
     3901 tttgcgggtg atgcgaaaag cggggctccg acaatcaaag gcatgctatt gttgacatga
     3961 accccctagc ttcgttagac tgctttgttt acgtctggtc gtccagactt tgggcagccg
     4021 aaggatctgt ccagccctcg acgttgagcg cagagggcgg gaccggggga aacataagcg
     4081 gaacgtgtcg ttaaccatcc ctggccttcg gcatctctgg tgtatgtccc tgtgaaatag
     4141 gccggcttgg aaaccgttgt gacgcgcagg gacgcagagg agtcatcacc aaaggtggtg
     4201 gttctctgtt tttgggtgaa cggccgggtc ttggtccgcg tacgaaagac ttaagttatt
     4261 cggtcgtcgc ggaatcctgc caagagacgt taatcgccga gaagaggccc ctttggttgt
     4321 acaggggaaa tttgtcggag actggtcaaa cgcggtttgg gatttgtgac aagcgaggtt
     4381 acgtatgaat gtgccgttga ggagtcgatg gtcttcagcc cgacgatggc tgaccttggg
     4441 agttgccctt ttggtgggcc tcctcagtta ccacctggtg tttgcggaag aagcaacgag
     4501 tgctgcccca gctagtccct cgctgggtgg ccagctcagt gtgttcgtgt ggtggattat
     4561 cgggttgggc ggttcaatct gcggtttgta ttttgcctgg cggttcttta aggaaatgct
     4621 caattccgaa aaaggaaacg agcgaatgga ggagatcgcc agctatgtcc gggaaggggc
     4681 caacgcctat ctgaaacagc aatatacggt agtggcatgc ttctttgtgg tgattgtcat
     4741 cctgttggca attgccgcat ttggtttgca tgcccaaagc cgttgggtac ccttcgcatt
     4801 tctgacaggt gggtttttct ccggcttagc cggttggttg ggaatgaaga ccgccacact
     4861 tgcgagtagc cgcacggccc agggctgccg caagtcgctc aatgatggct tgcgggtggc
     4921 gtttcgcagc ggtgccgtga tgggcctgac tgtggtcggc ttggggcttc tcgacatctg
     4981 cctgtggttt gccatcctct actgggtcat ctcggcattt ggatatcaca tgacgctgga
     5041 acagatcacc gtcacgatgc tttgcttcgg gatgggggcc agtagccaag cgctctttgc
     5101 ccgtgttggc ggtggtatct tcacgaaggc cgccgatgtc ggggctgacc tggtgggtaa
     5161 ggtagaaaag ggtattccgg aagatgatcc ccgtaacccc gccgtgattg ccgacaatgt
     5221 gggtgacaac gtcggcgacg tggcaggtat gggtgctgac ctgtacgaat cgtactgcgg
     5281 ttcgattctc gccacggcgg cccttggtgt ggcagccttc gctaccggcg agttgctccc
     5341 gactcgaatc ctggaacagg ttcaaaaagg cgcaattgcc ctcgaatcgg ccaaactcaa
     5401 ttaccaactt gccgctttgt tcctcccgat cgtcctggcg ggtgttggga ttgccctgtc
     5461 cgtgatcggt atttatgccg ttcggtcaga ggaagatgcg acccagagga cattgctcaa
     5521 ggccttgggc cgcgggatca atctgtccac gttgctcatt gccgtggcca cgtttgtctt
     5581 tacctatctt ctcatgcccg atttctggac tgtggcggtc agcgtgctcg tcggtttgat
     5641 tgcgggttgg ctcatcggca agtggaccga gtattccacc agcgatgaat accagccgac
     5701 gaagttcatt gccagccaat cgcagacggg tccggccacg gtgatcatcg gtggctttgc
     5761 ggaagggatg aagagcacgt ggttcccggt catcgtcgtg gcggtggcta cccttcttgc
     5821 ctttggtttt gcggctcagt tccacttcaa tgacctgacg tggtttgcca tggggctgta
     5881 cggtgtcggt atcgcggccg tgggtatgct gagcacactt ggcattacgc tggccaccga
     5941 cgcttacggt ccaattgccg acaatgccgg tggtaacgcc gagatgtccc acttggaacc
     6001 ccaagtccgg cagcgaacgg acgccctcga cagcctgggg aacaccacgg cggccactgg
     6061 aaagggtttc gccatcggtt cggcggccct gacggcgctg gcacttttgg cggcttatgt
     6121 ggaagaggtc cgcgttggtt tcgaccgctg ggcccattct gcccttgaac aagccgagcc
     6181 ggggctttat aagctgggcg acaaattctt ggccgaagtg acgggcgaga aaggccctgg
     6241 cggtaaaccg attattcatg gctatctcgt cttcggcagt gccatcaccg atcccgctgt
     6301 cgccgaggac tggaacaaac tggatgcctc tcgcgagccc caaaaaatcc ccgagggaat
     6361 catcgagcgg ttcgaggatg aagtgaaagg catcaagcgg acccgtctcc aatttgccaa
     6421 gacgaaggcc ccattgattc gggtggatcg ggcaacgatg cctgacttcg ccaactacta
     6481 cgaagcccac ctcatgaatc ccaaggtact ggtgggtgtc ctcattgggg ccatgttgcc
     6541 gttcctcttc tcctcgttga cgatgaaggc cgtcggtcgt gcggcctacc gcatggtcga
     6601 ggaggtccgc cggcaattcc gcgaaaagcc gggcattatg gaagggaccg aaaagcctga
     6661 ctacgctacg cctgtgcgaa tcagcacgca ggccgctcaa aaggagatga tcctgccctc
     6721 cttactgggg ctgctcacgc ctgtggccgt ggccttgatc cttggtgtga caggggtgat
     6781 gggccttctg atgggcgccc tggcctccgg cttctgcatc gcggtgttca tggccaatgc
     6841 cggtggtgcc tgggataacg ccaaaaagca cattgaacgt ggcgcttacg gcggaaaagg
     6901 caccgacgct cataaagcgg ccgtcgtggg cgacaccgtg ggcgacccgt tcaaggatac
     6961 cagcggtccc agcctcaaca ttctcattaa gctcatgagc atggtgagcg tggtggtggc
     7021 agggctggcc gtccgttaca gtcttgcgtc gctcggttgg ttctgatacg ggccccaaga
     7081 cctcgatgtg taaacatcga gccaggtagg tcatttcgac gaggcgggat ttacgtcccg
     7141 cctctttttt tctcgctttt tgtcgctacg caaacgccgg catcaggcga gttgctccag
     7201 ctcttcaaca cgcctggcga gttctaggac aagaggattc tcgctggccc aaccaagaac
     7261 ctcagccaca cggcgataat accagagcgt cccctcgcga ccgcccttga acttgttcca
     7321 aatctccggc ccaaagacgc ggtattgccc aagcaagatg ctgacgttgt ctagcttatc
     7381 cgcggcttgg atgaggcgga cggccgcgtt agcggtgcga aggcgttcca aaaaggcctc
     7441 ctttcgttcc cgccagggtg gcttgggctg aaccaccgta tcgctgcacg cttgaacaat
     7501 ggacagcaca gtctcgccaa agcgggcagc gatttcctcg gcgagtcccg gtcgattttg
     7561 atcctctagg gaatcgtgaa gcagagcggc aatggcctcg tcttcggtgc ccccgtattc
     7621 caaaacgagc ccacataccc gcaggaggtg tcctatgtac ggcgcatcgt tgacttttcg
     7681 tcgctggtca cgatgcaacg agacggcaaa ttgcagggcc tcatcaaaac ggggggtgag
     7741 attcatcgct ctttatcacg aaaatgggat gacttacggt gcgccgcctt cgtaaacact
     7801 aaattcggcc caaaccggca ggtgctgggc aacctgttca gcctgggctg tcgtcaattg
     7861 gaactgccgc aatagatcga caacccctcg gcgtcccgtg tattctatgg tggcacgacc
     7921 atcaaacaca atgttttgcg aagattccgt ccccgagaca ttgcttggca gacccatgat
     7981 cgcacatgtg ccatcgggca cgcgcagcgc tgccgtgatg acccgatagt cccgcgagaa
     8041 attgcccacg acgataacgt catcttcccc tcggccgtca tcccgcacgg cgcggaaaac
     8101 ctcgtcgagt aggcttaatt cttcgtccgc ccggaagggg tccacggagt agcttaccag
     8161 tgaaaaggtg aaggcctttt cgggaggcac ccctcttgcc cgaaaggcag ccactagggg
     8221 cggaacttgc agccgccttt gcggatcatc gaccacttgc actgtccggc ggtcaacctc
     8281 gagggcacgc atgtcaaaga ggacgaccgt cccccgccgc gtcaccaggc ggagcggttc
     8341 cggtaccgtc acataatgaa aggtctcgtt cttgggatcg atgtggtcga ggagctgcgc
     8401 caagacgctc cggtcccgaa gatccacccc ctgaagcaca gccaaatcga actggtgcac
     8461 aatcgtggca atggcggtga gcacgcctgg ttccaggacc ttccgttgat cgaggggcgt
     8521 cacatcccac acgacgatgc gaaaggccct ttgcttcttc gcggggggga gaatcgcaat
     8581 tggctcagcg ggtgtttcgc cggaggggga tgaaccggga cggatttcgc gaatgggtag
     8641 gtttcccttc gagagggtgt aaacctgcgg gataaccaac catgcgacgg caatcccgcc
     8701 aattaggagt agaagtgagt aaaaccgtgt catatcctca ccgcaatagg actaccaccc
     8761 aggcctaagg cagcggcccc accagggcta accgacatac aagcctgctc ctcacgcagt
     8821 ccaaaagcca gtcgctaccg tgacgtgttt gccagcgatc ggccgatttg tttgatggta
     8881 tcccctaatc catatcgtcg gttgacggga gaaacggatt cgcttgatac tgttcaagcc
     8941 ctctggtcgc taaaatcgtt gcaagtggca tcgacggttt gccgactgtt cgttgggaca
     9001 agcaaggtct cggttggccc ccaagtattg ggtccatcgc ctttccctgc gaagtatgac
     9061 aatggtaggg gcaaacccga tggtggccta tttgctgaat ttgagtcggc ggtgtgctac
     9121 gtcgcattaa aagcctatcc cataatctcc cctcgcccgt tcggcgggag aggggtcggg
     9181 ggtgaggggg ctcgaatcgc tgccggtctt ctcaattgat tccgacgttc gcttgccctc
     9241 accccaaccc tctcccggag ggagagggga ct
//
LOCUS       DAOSUT010000156         9258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_3987_length_9258_cov_8.539172, whole genome shotgun sequence.
ACCESSION   DAOSUT010000156 DAOSUT010000000
VERSION     DAOSUT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_9258_cov_8.539172"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLD05_12675"
     CDS             <1..624
                     /locus_tag="PLD05_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOP78343.1"
                     /translation="AVGTVAAVYLLTQFDTILGLSSLVPVEIAAYIVELLIWTTIRLG
                     IALLVLAILDFGFQYWKREQDLKMTPQEFREEMRNLEGDPQIAARRKVVQRQLMLNRV
                     NQAVPKADVVVTNPTELAVALQYIPEKMAAPIVVAKGAGLVAQRIRRLAEQHGVPVIE
                     KKPLAQALYRTVEVNQPIPQELYAAVAEVLAYVYHLKGKKPSIPKAA"
     gene            complement(666..1871)
                     /locus_tag="PLD05_12680"
     CDS             complement(666..1871)
                     /locus_tag="PLD05_12680"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOP78344.1"
                     /translation="MTKNGVKTKEVGGYRDRPWIPRFWDGITFLGWVRVMAHHRFRIA
                     PRRLGMAILITFLGLFNSFLALIQQAILGRKINRTKLPGDPIFIIGHWRSGTTLLHEM
                     FILDPRWGFPDSYACFAPNHFLISRCFLAPLLGLLMPKMRPIDNMPFDWNRPQEDEFA
                     LCNMGLPSPYLSLIFPNEKPRYDAYLTLEGLTEKQLSRWKRTFLWFLKAVTVRNPRPM
                     VLKSPPHTCRIRVLLEMFPQAKFVHIYRDPYAVFPSTVNLWKRLSTDEALQIPHHQGL
                     EERVFDIFCKMYEVFERDRELIPPHQFAEVSYEALVADPEEEMERLYRELDLGDFEIL
                     RPRLRQFIAERSNYKRNRFELAPALKNEIARRWGFFFQKYGYSIEQTEAAESVPSANV
                     TSPQKNPLG"
     gene            2490..3797
                     /gene="purD"
                     /locus_tag="PLD05_12685"
     CDS             2490..3797
                     /gene="purD"
                     /locus_tag="PLD05_12685"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOP78345.1"
                     /translation="MNVLVIGSGGREHALAWKLKQSSLVDRVFVAPGNAGTAEDAENV
                     PIPETDFAQLIKFVKENGVDLTVVGPEALLAAGIVDAFRKEKLRIFGPNREAAQLESS
                     KVFCKQLLRSGNVPTAEFQVFSDPESARRYLVDREDVPIVVKADGLAAGKGVIVCKNR
                     EEALEAVERIAVRKVFGAAGNRLVIEEMLHGEEASVLAITDGKTIVTLQPAQDHKPAY
                     DGDTGPNTGGMGAYSPAPVVTDEMLHWIEANVLVPTVHAMKKNGCPFQGVLYAGLMIT
                     PLGPRVLEYNVRFGDPECQPLMMRLKSDLAAILDAAVDGRLDRIESLEWDPRPAVCVV
                     VASEGYPGEYKKGFPIHGLEEAAKIPDVKVFHAGTTIRDGQVVTSGGRVLGVTALGDN
                     IARAKYQAYAAVKCISWPGKWCRKDIADKAINRLTQSGANRNS"
     gene            complement(3879..4772)
                     /locus_tag="PLD05_12690"
     CDS             complement(3879..4772)
                     /locus_tag="PLD05_12690"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOP78346.1"
                     /translation="MRPTQLWLTASLSKSLPQTRGLLCPDNGTKAAMIRFLIDGYNFL
                     NASGIDTQRFDFSGTTPLERSRLALLDFLVTHLTPEEISETVVVFDARHFLRPQADEF
                     TYHGLTVRFAARYPDADSLLEEMIQSHSAPKQLTVISSDHRIQRAARRRRAKFIDSES
                     WYRHLCARHHRQGTTIQSKAGTEDKEVVNLSPHELKHWLEEFASSCKLSEETPHSVSE
                     TRRPESNDREHPTSSNSGDEAVPGKSDVTDTMPNLTAYSVEEWAKHLGVSPDKLNLPI
                     DAFKDIETPNKTQHGKRKRRQ"
     gene            complement(4769..6049)
                     /locus_tag="PLD05_12695"
     CDS             complement(4769..6049)
                     /locus_tag="PLD05_12695"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOP78347.1"
                     /translation="MERLLIRGGRVIDPSQGLDREMDILIQGGQIVEYDPPVRGDEVV
                     IDAKGKIVAPGLIDMHVHLREPGQEDDETIATGTAAALAGGFTSIVCCPNTDPPIDSP
                     ATVEFVRQKAARADNCHVFVMACVSKDRKGQELAEIGQLVAAGAVAFTDDGAPVYDPE
                     LMRRAFEYCLMFDKPVCNHCEIPELARNGVMHEGLVSLILGLPGIPAAAESVMVARDI
                     ALAEATGGRLHVMHVSTLDSVEAIRRAKKRGVRVTAEVTPHHFTLTDECLRTFDSNYK
                     MNPPLRSRDHVEACINGLRDGTIDVIASDHAPHAKEKKMQELDRAPFGIIGLETSLSL
                     VITKLIKPGLLTWSEALAKMTINPARILGLNKGTLAIGADADVTIIDPNYQWVVDPSA
                     FLSKARNTPFAGWKLEGRATEVIVSGTIKYRIGP"
     gene            complement(6113..7075)
                     /locus_tag="PLD05_12700"
     CDS             complement(6113..7075)
                     /locus_tag="PLD05_12700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOP78348.1"
                     /translation="MDDIESKLAPFRGRWNRSHLLSLEELSREELLCILDAADVFRDA
                     FDRGLRKWPVLTGKTCVNLFFENSTRTRNSFSLAARRLGADVVDFAASTSSLSKGETV
                     VDTARNLEAMGIEAVIVRHSTPGTPHLLANHLRCSVINAGDGAHEHPTQGLLDIMTIR
                     RRLGRIEGLTVALIGDIAHSRTARSNIWGLTKLGAHVIVCGPSTLVSPLWRELGVEYS
                     YNLDDILPRVDVLNLLRIQFERQTRRPFPSVREYASLYAMNAARLSRAKPDILIIAPG
                     PINRGVEITADVADGQHSAILDQVTNGVAVRMAVLWLLITRQKE"
     gene            7291..8325
                     /locus_tag="PLD05_12705"
     CDS             7291..8325
                     /locus_tag="PLD05_12705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOP78349.1"
                     /translation="MSTRPRFIVYVIGSQGMVQRIEAALRPVKLISEVIAVRDDALHS
                     QGDSTALTEDADTNGTSYEATLALLVTDTTWDKLKLLALCRRYMPYRHDPRLTAVLRV
                     GTREGQGTHISAGCHAADLRAIVSLLRRNLELRHYLLEKQSLVKALHSMAVRDPLTGL
                     INRRGWKIGIKKLWQEAAGRDGFLAVALFDLDGFKKINDQFGHAFGDHVLKTVAAQAQ
                     KACRKGDLLARWGGDEIALAVVLPDKAVARGLVERVQNSLRSDFPQIGQLVTACAGYV
                     VVKVVTTWRPDFGKRLLEAADAVLTQAKASGTGRVCEGNVVLSDEEKSIADKSPGGAG
                     DSSTDEHSES"
     gene            complement(8322..>9258)
                     /locus_tag="PLD05_12710"
     CDS             complement(8322..>9258)
                     /locus_tag="PLD05_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP78350.1"
                     /translation="STMITLHHFTKTYGRTIAVNDLTMEVPFGEILGFIGRNGAGKST
                     TIRFLATLIKPTAGTGTVAGFDVVRQPIEARHHLGYMPDMFGDYPGFRVRTFLEFFAT
                     GYGLVGKSRRHRVNDVIERLGLDDYVSRPVRTLSRGMKQRLYLARCLLHDPPVLILDE
                     PMNGLDPEARMEFRNFLCQLAASGKTILLSSHILGELADTATSVAIIHRGNLVAHGGV
                     SEVVRDYTTGTRVVVRFLQPQRLSQEAIEALHPHVKLLSVDSSEALLQVHGGEELLAQ
                     VLQELVRRGMQVSAFHPLEPTLEEFFVRLTQNRQG"
BASE COUNT         2130 a         2451 c         2616 g         2061 t
ORIGIN      
        1 gcggtgggaa ctgtcgccgc cgtgtatttg ttgacacaat ttgacacaat cctcgggtta
       61 agttcccttg tccccgttga gatcgcagcc tacatcgtag aactcctcat ttggacaacg
      121 attcgtcttg gtattgcctt gcttgtactg gccattttgg atttcggctt ccagtactgg
      181 aaacgcgaac aagacctgaa gatgacgccg caggaattcc gagaggaaat gcggaatcta
      241 gaaggtgatc cccaaattgc ggcgcggcga aaagtcgtgc aaaggcagct tatgttgaat
      301 cgggtcaatc aggccgtacc caaggccgac gttgtggtga ccaaccccac ggagttggcc
      361 gtggcgctgc aatatatccc ggagaaaatg gcggcaccaa tcgtagttgc taaaggagcc
      421 ggtttagtag cccaaaggat ccgtcgcctc gcggaacagc acggtgtgcc tgtcattgaa
      481 aagaaacccc tcgctcaggc cctttatcga acggtggaag tcaaccagcc gatcccgcag
      541 gaactgtacg cagccgttgc agaagtcctt gcctacgtct atcacttgaa aggcaaaaag
      601 ccctctatcc ccaaagcggc ataatcactg acatgaggtc gccttctgtc ggagatcaaa
      661 ttcggttagc ccaggggatt tttctgcgga gacgtcacat tggcggaggg cactgattcg
      721 gccgcttctg tttgctcgat ggagtatcca tatttctgga agaagaatcc ccaccgccgc
      781 gcgatttcat tcttcagagc gggcgccagc tcaaagcgat tacgcttgta attggatctt
      841 tcggcaatga actgcctcaa acgaggacga aggatttcga agtcacctag gtctaactcc
      901 cgatagaggc gttccatttc ttcctcggga tccgccacaa gggcctcgta actgacctcc
      961 gcgaactggt gcggggggat gagttctcga tccctttcga agacttcata catcttacaa
     1021 aagatgtcga agacccgttc ctccaagccc tgatgatgcg gaatctgaag ggcctcgtcc
     1081 gttgagagtc gcttccacag attgaccgtc gaagggaata cggcgtacgg atcgcgatag
     1141 atgtgaacaa acttcgcttg cggaaacatc tccagaagca cgcggattcg gcaggtgtgc
     1201 ggcggagatt tgaggaccat aggccgcgga ttccgtacgg tcacggcctt aagaaaccat
     1261 aggaatgttc gcttccatcg actcagttgt ttctccgtga gcccctctag cgtcaagtag
     1321 gcatcgtaac gtggtttttc gttgggaaag atcagactca aataaggcga aggcaaaccc
     1381 atgttgcaga gggcaaattc gtcttcctgc ggcctgttcc aatcgaacgg catgttatcg
     1441 atgggccgca tcttgggcat caaaagcccg agaagtggag ccaggaaaca tcgcgagatc
     1501 aaaaaatggt tcggtgcaaa acaggcataa ctgtcgggaa agccccaccg aggatcaaga
     1561 ataaacatct cgtggagaag cgtggtcccc gatcgccagt gaccaatgat gaagatgggg
     1621 tcgccgggca gtttagtccg gttaatcttc cttcccaaga ttgcttgctg aatcaaggcc
     1681 agaaacgagt tgaacagccc aagaaatgta atcaggatcg ccattcctag gcgacgcggg
     1741 gcgattcgga agcggtggtg ggccatgacc ctaacccacc caaggaaggt aatgccatcc
     1801 cagaagcgtg ggatccatgg tcggtcgcgg tagcccccaa cttcctttgt tttgactccg
     1861 ttttttgtca tcacactatt ccatcgcgag ggaggttttc gcgcaacttg tgcagaagcc
     1921 cccagagaac tttaatgcgg gacttttatg ggtgaggttt gacatgatct gagaaagcta
     1981 cctgagttgc ccaaaacgat tattttactc tattgtcccc gcatttccta cggagagctt
     2041 tcgtagactt tgccgaaatc ataaagttta ttcgggcatt tccgtgctaa atcctcaatt
     2101 ggatttgtct cgagcggtca ccagcggaaa aaggcaagcg cccgtaggac cgccgggcca
     2161 atccgaaact ccgccgaacg cctggtttgt tagaaagctg aagcactaag agcctatgac
     2221 atcatctccc ctcgcccgct ggccgggaga ggggtcgggg gaagggtagc tcgaatcgct
     2281 gccggtttcc tcgatcaact ccgacgttcg cttgccctca ccccaaccct ctcccggagg
     2341 gagagggggg actttttggg aaggctctaa acagcgtgct aggtgacctg cctcacacag
     2401 gcatcctttc caggcttagc tgcattccct agaatcagca tttggtcact tgtgagagct
     2461 tgagataggc tatttggagt tgtcggtcaa tgaacgtctt ggtgatcgga agcggtggac
     2521 gtgagcacgc ccttgcgtgg aaacttaagc aaagctcatt ggtggatcgg gtgtttgtcg
     2581 cgcctggcaa tgccggaacg gcggaagatg ctgagaatgt cccgattccg gaaaccgatt
     2641 tcgcgcagtt gatcaagttc gttaaggaaa acggtgttga cctgacggtt gtcggcccgg
     2701 aagcgctgtt agcggccgga atcgtggacg ccttccggaa ggaaaaactc cggattttcg
     2761 gtccgaaccg cgaggctgcg cagttggagt cgagcaaggt cttttgcaag caactcctgc
     2821 gaagtgggaa cgtccccacg gccgagtttc aggtgttctc tgatcccgaa tcggcacgcc
     2881 ggtatctggt cgatcgcgag gacgtcccta tcgtcgtcaa ggcggatgga ttggcggcgg
     2941 gaaagggggt catcgtttgc aagaaccggg aagaggccct ggaggccgtg gagagaatcg
     3001 ccgtaagaaa ggtttttggg gctgcgggga atcggttggt catcgaggag atgttgcatg
     3061 gcgaagaggc cagtgtcttg gcaattactg atggcaagac gatcgtcacg cttcagcctg
     3121 ctcaagacca taagcccgcg tacgacggag ataccggccc caacacgggc ggcatgggcg
     3181 cctacagtcc ggcgcccgtg gttaccgacg agatgcttca ctggattgaa gcgaacgtct
     3241 tggtgccgac tgttcatgcc atgaagaaaa atggttgccc gttccagggg gtactttacg
     3301 cagggttgat gattacgccg ttaggtcctc gagtgttaga gtacaacgtt cgttttgggg
     3361 atcccgagtg ccaacccctt atgatgcggc tcaaatcaga tcttgccgcg atccttgacg
     3421 cagcggttga cggtcggctg gaccgcattg aatccctcga gtgggatccc cgccctgcgg
     3481 tttgcgtggt agtggccagc gagggttatc cgggcgagta caagaaaggg ttccctattc
     3541 atgggctgga agaagcggcc aagattcccg atgtgaaggt cttccatgcc gggacaacca
     3601 tccgggacgg gcaggttgtg acctcgggtg gacgtgtctt gggggttact gccctcgggg
     3661 ataacattgc tagggccaag tatcaggcct acgctgccgt aaaatgcatc agttggcctg
     3721 gaaaatggtg tcggaaagac atcgctgaca aggcaatcaa ccgtttgacg caatcgggcg
     3781 ccaatcgcaa ttcttagatt ggtaccttgg gatgggtccg atcgaaatcg ccttcggcaa
     3841 tcgccagtca aaaacttgct gccgatggca acgcctattt attgacggcg ttttctttta
     3901 ccatgctgtg ttttgttggg tgtttcgatg tccttgaaag catcaatcgg aagattcagc
     3961 ttgtcaggtg agacgcccag gtgtttggcc cattcttcaa cggagtaagc cgtaagattt
     4021 ggcatggtgt cggtcacgtc cgatttgccg ggaacagctt cgtccccgga gttgctgctt
     4081 gtagggtgtt cccgatcgtt ggattctgga cgccgtgttt cactgacaga atgaggtgtt
     4141 tcctcggaaa gcttgcacga cgaagcaaac tcttctaacc agtgctttaa ctcatgaggg
     4201 gaaagattca ccacctcttt atcctcggtt cccgcctttg actggatagt cgttccttga
     4261 cgatgatgtc ttgcacacaa gtgacggtac caagattcgc tgtcaataaa cttggcccta
     4321 cgtcggcgtg ccgccctttg gatacgatga tcacttgaaa tgacggtcaa ttgtttcggg
     4381 gcagaatgag attggatcat ctcttccaga agtgaatccg cgtcggggta gcgggcggca
     4441 aagcgaacgg tcaagccgtg ataagtaaac tcgtccgctt gaggccgaag aaaatgccgg
     4501 gcatcgaaga ccacgacagt ttctgagatt tcctcgggcg taagatgggt gacaagaaaa
     4561 tccaataacg ccaagcgact acgttccaaa ggggtagtcc cggaaaagtc gaagcgctgg
     4621 gtatcgattc cggatgcatt aaggaaattg taaccatcaa tcaaaaagcg aatcatcgcc
     4681 gcttttgtgc cattgtcggg gcaaagcaaa ccccgggtct gcgggaggct cttgctaagg
     4741 gacgcagtta gccacagttg ggttggcctc aagggccaat cctgtactta attgtacccg
     4801 aaacaatgac ctcggttgcc cgaccttcga gtttccatcc cgcgaaggga gtgttgcggg
     4861 cctttgagag aaaagcactc ggatcaacca cccattggta gttaggatca ataatggtca
     4921 cgtcggcatc cgccccgatg gccagcgttc ctttgttgag accgagaatc ctggcgggat
     4981 tgatcgtcat cttggccaag gcctccgacc acgttaaaag ccccggtttg atcagtttgg
     5041 tgatgaccag actcaggctt gtttcgagcc caatgatccc gaagggggct cggtccaatt
     5101 cctgcatctt cttttcttta gcatggggtg cgtgatccga ggcaatcacg tcgatcgtcc
     5161 catcgcgcaa accgttgatg caggcctcca catggtcccg ggaccgtaat gggggattca
     5221 tcttatagtt tgaatcaaaa gtcctcagac attcgtcggt tagggtgaaa tgatgcgggg
     5281 ttacctccgc ggtgacccga actccgcgtt ttttggcacg acgaattgcc tccacgctat
     5341 ccaaggtgga cacgtgcatg acgtggagtc ggcccccggt ggcctctgct aaggcaatat
     5401 ctcgggcaac catgacggac tccgctgccg ccggaatccc agggagacca aggatcaggg
     5461 acacgaggcc ctcgtgcatg actccgtttc gggccaactc tgggatttcg caatgattgc
     5521 agacgggctt atcgaacatc aggcaatact caaaggcccg tcgcatgagt tcaggatcat
     5581 agacaggcgc gccatcatcc gtaaacgcga cagccccggc ggcaaccaat tggccaatct
     5641 cggccaactc ttgccccttg cggtccttgc ttacacaagc catgacaaag acatggcagt
     5701 tatcggcccg tgctgccttt tgccgaacaa actccacggt tgccggcgag tcaattggtg
     5761 gatcggtgtt tgggcagcag acgatggacg taaaaccgcc ggccagggcc gcggctgtgc
     5821 ccgtggcgat tgtttcatcg tcctcctgtc ccggttcccg gagatggaca tgcatgtcga
     5881 tgagcccggg cgcaacaatc ttgcctttgg catcgatgac gacttcgtcg ccacggacag
     5941 gcggatcgta ttcaacgatt tgcccgccct gaatgaggat gtccatttcc cgatccagac
     6001 cctggcttgg gtcgatgacc cggccaccac gaattaggag tctctccatg gtcgttcacc
     6061 tggcagatat ctgcatcgtt tcttcgttct ctcagaattg gtcgagtcca agctattctt
     6121 tctgtcgcgt tatgagaagc cacagaacgg ccattcgtac agccacacca ttggttacct
     6181 ggtcgagaat ggccgagtgc tgaccatcgg cgacatcggc cgtgatttcc acaccccgat
     6241 tgatgggacc tggggcaatg atcaagatgt cgggcttggc ccgcgataac cgcgcggcgt
     6301 tcatggcgta caaactcgcg tattcgcgca ccgaggggaa ggggcgacga gtttgtcgct
     6361 cgaattgaat ccggaggaga ttcaagacgt ctacccgggg gagaatatca tccaaattgt
     6421 aggagtactc gacccccaat tcccgccata gaggagatac aagcgtggac ggaccgcaaa
     6481 caatcacatg ggcccccagt ttggtgagtc cccagatgtt cgagcgtgct gttcgactat
     6541 gggcaatgtc tccaatcagg gccacggtga ggccttcgat acgtcccaag cggcgccgga
     6601 tcgtcatgat gtcgagcaac ccctgggtgg gatgctcatg ggcaccgtcg ccagcattga
     6661 tcaccgaaca tcgcaagtga ttggcgagga ggtgcggtgt ccccggcgtg ctatgtcgaa
     6721 caatgaccgc ctcaatgccc atggcttcca gattgcgggc ggtatccaca acggtctcgc
     6781 ccttggatag gctgctggtc gaggcggcga agtccacaac atcggccccc agccgccgag
     6841 cagctaagga aaaactattg cgggtccgtg tggaattctc gaagaaaaga ttgacgcacg
     6901 tcttgcccgt cagaacgggc cattttctca gcccccgatc gaaggcgtca cgaaacacat
     6961 cggccgcgtc aagaatgcaa aggagttcct cccgggacaa ctcctcaaga ctcaagagat
     7021 gagacctatt ccagcgcccc cggaatggcg ctaattttga ctcgatgtca tccatacaga
     7081 gaactcgcac caaaaaggct gaacgttcct caggtcacca gcgataccca tcatgtcgga
     7141 agaccataga taccatcggg cccaattggc gtaaaatcaa cctagccgac gattctacga
     7201 cttaatactc agtactgcaa ggaccacgat ttcggacgtg tttggaagat ccgtaaatcc
     7261 ccgctgaatg atcctcttag aggacacgtc atgtccacac gacctcggtt tatcgtttac
     7321 gtgatcgggt cccaaggcat ggtgcagcga atagaagcgg cactccgtcc tgtgaaactg
     7381 atttcggaag tcatcgccgt tagggacgac gctttgcaca gtcaaggcga cagcaccgct
     7441 cttaccgaag acgcggacac gaacggaacc tcgtatgagg caactttggc cctcctggtt
     7501 accgacacca catgggacaa gcttaaactc ttggcacttt gcagacgcta tatgccatat
     7561 cgccacgatc ctcggttaac cgctgtgctg cgggttggca cgagagaagg gcaaggaact
     7621 cacatttcgg cggggtgtca tgccgcggac cttcgcgcca tcgtgtcgct gttacggcga
     7681 aacctggaat tgcgccacta tttactggaa aagcaatccc tcgttaaagc cctacactcg
     7741 atggccgttc gtgatcccct aacggggttg atcaaccgtc gcggctggaa gatcggcatc
     7801 aagaagttat ggcaggaagc cgcaggacgt gatggctttt tggcagttgc cctgtttgat
     7861 ttggacggtt tcaaaaaaat caatgaccag tttgggcatg cctttggcga ccatgtccta
     7921 aagactgtgg cggcacaggc ccagaaagcc tgtcgaaagg gggacctttt ggcccgttgg
     7981 ggcggtgacg aaatcgcttt ggccgttgtt ttacccgaca aagctgtcgc tcggggcctt
     8041 gtcgaacggg tacaaaacag cctgcgatcg gattttccac agatagggca gcttgtcact
     8101 gcgtgtgcag gctacgtggt cgtgaaggtt gtcacaacct ggcggccaga cttcgggaaa
     8161 cgccttctgg aggctgcaga tgcggtcctc acccaggcca aagcctctgg gacaggtcgc
     8221 gtttgcgagg gaaacgttgt tctttccgat gaagagaagt cgatcgccga caaatccccc
     8281 ggtggggcag gggacagcag cacggacgag cacagcgagt cttagccttg acgattttgt
     8341 gtcaatcgca caaagaactc ttcgagcgtc ggttctagcg ggtgaaatgc acttacctgc
     8401 atgccacgac gaaccaattc ctgaaggacc tgcgccaata gctcttcacc accgtgaact
     8461 tgcaacaagg cctcagaaga atcgaccgac aaaagcttca catgaggatg gagggcctcg
     8521 atggcctctt gggataatcg ttgtggctgg agaaatcgaa ccacgacccg agttcccgtg
     8581 gtgtaatccc gaacaacttc cgatacgcct ccatgagcga cgaggttgcc ccgatgaata
     8641 atcgcaacgc tcgtggcagt gtcggcaagc tctcccaaaa tgtggctcga gaggaggatc
     8701 gtctttcccg atgccgccaa ttgacaaaga aagttcctaa attccatcct cgcctccgga
     8761 tcgaggccat tcattggctc atccaggatc aaaacggggg gatcgtggag aagacacctt
     8821 gccaggtaaa gacgttgttt catcccgcga gaaagcgtcc gaactggccg ggagacgtaa
     8881 tcatctagcc caagtcgttc gatgacgtcg tttacacggt gtcgtcgtga ttttccaact
     8941 aaaccgtaac ctgtcgcaaa gaactcaagg aacgtacgga cgcgaaagcc aggatagtcg
     9001 ccgaacatgt ccggcatgta accgaggtgg tggcgggcct caatgggctg gcgaaccacg
     9061 tcgaaaccgg caaccgtgcc cgtccctgcc gtcggtttga tcagtgttgc caagaaacga
     9121 atggttgtcg atttgcccgc cccatttcgg ccgatgaagc caaggatttc gccaaaggga
     9181 acctccatgg tgaggtcatt aacagcaatg gttcggccgt aggttttggt gaagtggtgt
     9241 agtgtgatca tcgtgctc
//
LOCUS       DAOSUT010000157         9206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4011_length_9206_cov_10.516556, whole genome shotgun sequence.
ACCESSION   DAOSUT010000157 DAOSUT010000000
VERSION     DAOSUT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_9206_cov_10.516556"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..770)
                     /locus_tag="PLD05_12715"
     CDS             complement(396..770)
                     /locus_tag="PLD05_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78351.1"
                     /translation="MDFRSSDPLKTGALLVSLGSAPVAVGRVFPQPGRPAFLRRGVAQ
                     LQKEVNLTLPQQGLIRSIFFWTLACVVRVAGYLSDFFSRRKIIGASLLFRRLSTSCMG
                     LARDWIHLVFFRSVVSGGGEAF"
     gene            complement(743..3100)
                     /locus_tag="PLD05_12720"
     CDS             complement(743..3100)
                     /locus_tag="PLD05_12720"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOP78352.1"
                     /translation="MKNRYEKHTRWARGLLAGVLWLQMCVGFAEAEAGELVGKVTDPQ
                     GKPIAGARLYLVDTIRGIERSSITDPTGHYRIASLSPGEYLWQSFCVGYRTLRGRLSL
                     PESNQPLTHDLELPSAQEYRLENEKLLLVIDENGTVRELRNKQVDASVNYVSGQAPGF
                     WHIVFRRGECWENVVLAEAQQYQIEKPAQNRLVLRVDRLRFRQETFPVTIQFSIQLHE
                     DELSWAATVENRAELEITEFYFPELGGVRSLGGKSGKEYLYWPNGLGERMDGFASRTL
                     RMTYPWPASMAWFTVCNGQEGIYCGAHDDSFLSSELQVGPNPLQANEPTLGFVKFPFI
                     RPGETWTSKPYVVACYKGTWHTAADKYRRWMHTWRKTLEKPAWVQRTTGMFLVILKQQ
                     YGDIMWHYDEIPLLYEEAQRNGINMVALFGWTQGGHDNQYPQFLPDPKMGGAEVLRRA
                     LQQVRASGGHVVCYMQGRLLDAQSEYVQSPEGQKVVARNLWGGPYVEEYNKYYHSSLL
                     RLYSRKLQMIANPIHPEWRTTLLEKAQEVIQHGPNGILFDQIGGLYAYPAFDTPPEIK
                     PTLAFPEGQQKLLAFLRTQLKQKQSDFGLLSEHLTDVYSQYVDMLHGCGAGYAPGPTS
                     FPAMARYVLPDVILTSRNPMPRLDARQVNFALAYGLLPELEVRYRNDASIIRDGVGAE
                     KAEYLRKIGQLRGRYPELLFQGRYMDVLGVEASNPAVTVTSFESGRRRAVVAWNDTSS
                     EQRIEVAMPGWHLVEVAGLEQTLPPSPQKLAPQEVAVWIFDRQTP"
     gene            complement(3534..5561)
                     /gene="brxL"
                     /locus_tag="PLD05_12725"
     CDS             complement(3534..5561)
                     /gene="brxL"
                     /locus_tag="PLD05_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HOP78353.1"
                     /translation="MPELDRKAVSVFAGKVVRKDLVRKVKVGANVPVFVLEYLLGKYC
                     ATDDAAAIEAGLRVVNSIIASNFVRPDEANKVQSMVKDKGRHTLIDKVKVRYLSDDDK
                     HWAELVNFGHKYVHVPEHFVREYDRLLMGGIWAQVEIRHQYDEEAKGKRSPFWIDSLK
                     PIQIATFDLAEYCACRREFSTDEWLDLLLRTIGLEPSHFDRRLKMLLLVRMIPLCEHN
                     YNLVELGPRGTGKSYAYQELSPYTILLTGPTTVANLFYNMATGKMGLVGIWDAIAFDE
                     VADLQKMPKEVVTTLKTYCESGTFARGKDALTGMASVAMFGNTNQSVDVMVRSSHLFQ
                     PMPAVIREDMAFLDRLHFYVPGWEIPKMRVEFFTDHYGLVVDYFAEALRELRKHNFTE
                     AIDRFFSLGSHLNARDVKAVRKTVSGLIKIIYPHGEFSRNELAELLELAMEGRRRVKE
                     QLKKLGAFEYHQTSFSYMDNETREERFVGVPEQGGQDIISPDPLAPGSVYTASVDDQG
                     RVGLYRLEVSCSSGTGKLRVAGGIDGTMRESIQRAFAYLIAQKSKIGLEQQLDSTDFH
                     VEGIDLLGNHVPCEAGIGFIVAVYSALKKQSVLAGLLILGDLSIQGNIKSLRSLVEAL
                     QVGMDNGARRALIPLENKRNFLEVPGDIVERVDPIFFSDPMTAAMKALGIA"
     gene            complement(5572..8166)
                     /locus_tag="PLD05_12730"
     CDS             complement(5572..8166)
                     /locus_tag="PLD05_12730"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOP78354.1"
                     /translation="MKPGLVTEYLLGLVRKQVEDHGLVVWYDPDGVYADAVEVLELPG
                     TTVLRYEGSFFDLRWRIDQKGLMDGEEPPRLVVYVPLAQDKTHHALIELEVAGVVMQP
                     GQQPPARNTRLAVVARKALRGVLSDEAAAEIEKQVEAGKLTLADLDALAAKGGEVSKG
                     LLTLIFGTGNPQEIALAFLTHRSHDEEINKKEAGEELRKLLRQEFDLTTFDHASLDDI
                     RCLLSRHVLLTELVVGLGQALPSRLASVPVASTSEGRKACVDLAKAWRLRWDLRESYV
                     TAARRVEGEFALDPTQFDPRAIWELETFPAIEEALLQYAEKRLLENTDGEVLTLAEAR
                     QKQFWCEAEPQFQARWALIALAAEVLLEAQRVEQALKKAPASLAGLIKEYAEGSQPWC
                     HLDTCHRHMESRWYNFEPHGRDDENLEKLIIRARQRYVAVGSELARIFVTRLRETFPD
                     KGVIRQREVFEKYVKPALGNEKVAYVWVDALRFEMGRELARLMGDDFEVEVQPALAAA
                     PTLTEIGMAALLPGAHEAAKVVTAGSGRLALEIGGSVVKDRKDRVAFLKDRAGVAVFD
                     AKLDDLLPKPTKKIREGISNAQLVLVTSQEIDELCEQDNITQARRQMDGVLHDLRRGI
                     RVLAELGVERIVLASDHGHLFAEELSEDMKVEAPGGETADLHRRVWVGRGGQANDATL
                     RMPLTAVGMEGDFDVATPLTFACFKCKGGARAYFHGGLSPQEALIPVLVLKPLAKTAV
                     TGPTAIIWQLLPGSQKLTTRFFSVQVTGTSQGLFDVEPPKVRVELRAKGRAISRAVSA
                     SYGFEESTGDVALKRDDADPKKIVPNTVTLMIVEEPDQKTVGLYLFDAATGAELSKLE
                     KIEVAIAM"
     gene            complement(8199..>9206)
                     /locus_tag="PLD05_12735"
     CDS             complement(8199..>9206)
                     /locus_tag="PLD05_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78355.1"
                     /translation="EEEEPGDNDSEETETTKTFWSEEDLAKAWISYAVGIVMGQFEPG
                     KEKGLGRGRFDTATAGKLQELSDPDGLLVVDSTHSDDLAKKVFAALEVIFGEAKAESL
                     IRQALGNHGELVTTLRDYLADKFFKEHIQKYRKRPIYWYLRSAKGNYGLWLYYHRLDK
                     DILFKALLNYVEPKIRLEEDRLSTLRARREAAGKSGREAKQIEKEIDRQEQFVAELQD
                     FRDKLRRAADLHITPDVNDGVILNIAPLWELVPWQEPKKYWEALQEGKYDWAHIAMQL
                     WPERVKEKCRTDRSLAIAHGLEHLCEAPPPKTSRSALRAENPDEDQKELEEESADDEA
                     E"
BASE COUNT         1866 a         2884 c         2504 g         1952 t
ORIGIN      
        1 caccggcggg tagttgccca ggttcgcgga acgtccccaa atgaaaaagg cgtgccccgg
       61 caagagcacc cgggccatct cgccaaacca ggcgtgtaga aggcggtcaa attggtcgtc
      121 gcccctcact tgtcctctaa cagggggaga gtgttccgcc tagggggagc ggcccctgta
      181 ggtgggtccc gaagccaaag taaacacagc accgcccaga gtaggcccac tcccccgaag
      241 ttccaaagcg ccgcgcgtca gccccaccga tccgccactt agccgccaaa aaaacgctgg
      301 caatcacccc ataataaggg aggtttgatg aatcgataaa gctaacgagc ggttttttgg
      361 tggaagtcgc cgatcagagc gttggctgcc ggggcctaga aggcttcgcc tcctccggag
      421 accacgcttc ggaaaaacac caagtggatc caatctcggg ccaagcccat acagcttgtg
      481 gacaacctcc ggaacaagag gctcgcaccg atgattttcc ttcggctgaa gaagtcgctc
      541 agatagcctg ccacccgcac cacgcaagcc aacgtccaaa agaagatcga cctgatcagg
      601 ccctgctgag gcagggttaa gttcacttct ttctgaagct gggcaacacc acgccgtaga
      661 aaggccggtc ggcctggttg aggaaaaacg cgtcccacag ccacgggagc acttcccagc
      721 gataccaaaa gggcaccggt ttttaggggg tctgacgatc gaaaatccat acggcgacct
      781 cctgaggagc cagtttttgc gggctgggcg ggagcgtttg ttcgagtccg gccacttcca
      841 caagatgcca gccgggcatg gccacttcga tcctctgctc gctggaagtg tcgttccagg
      901 caaccacagc ccgacgtcgc cccgattcaa aactggttac cgtaacagcc ggattagagg
      961 cctccacgcc cagcacgtcc atatagcgtc cctggaacag aagttccgga tagcgaccgc
     1021 ggagttggcc gattttacgg agatactcgg ccttttcggc cccgacccca tcccggatga
     1081 tcgaggcgtc gttccgatat cggacttcca gttcgggcag cagtccgtag gccaaggcga
     1141 aattgacctg tcgggcgtcg agccgtggca tggggttgcg agaggtaagg atgacatctg
     1201 gcaagacgta tcgcgccatt gctgggaaag acgtcggtcc aggtgcgtac cccgcgccac
     1261 atccatgaag catatcgaca tattgcgaat agacatcggt caggtgttcg gagagcagac
     1321 cgaaatccga ttgcttctgc ttgagctggg tacgaaggaa ggcgagcaac ttttgttgtc
     1381 cttcgggaaa ggcgagagta ggtttgatct ccggcggggt gtcgaaggcg ggataggcgt
     1441 acagaccgcc gatttgatcg aaaaggattc cgttcgggcc gtgttggatg acctcttggg
     1501 ctttttccag gagtgtggtc cgccattccg gatggatcgg attggcaatc atctggagct
     1561 ttctggagta aagccgcaga agactactat ggtagtactt attatactcc tcgacatagg
     1621 gaccgcccca gagattgcgg gccaccactt tttgaccctc tgggctttgg acatactcgc
     1681 tttgcgcgtc caggagccga ccctgcatat agcagacgac atggccgccg ctggctcgca
     1741 cctgctgaag cgcgcgacgc aagacctctg cccctcccat tttgggatcg ggcagaaact
     1801 gaggatattg gttatcatgg ccgccttggg tccacccgaa aagggccacc atattgatcc
     1861 cattgcgctg agcctcttca tataaaagag ggatttcgtc atagtgccac atgatgtcgc
     1921 catattgctg tttgagaatg accagaaaca tgccggtcgt gcgttggacc caggcaggtt
     1981 tttcgagcgt tttgcgccag gtgtgcatcc aacggcgata cttgtccgcg gcggtatgcc
     2041 aagtcccctt gtaacaagcg acgacatagg gtttgctggt ccaggtttca ccaggtcgga
     2101 taaacggaaa cttcacaaac cccaaagtgg gctcattggc ctgaagagga ttcggcccca
     2161 cttgaagttc actacttaaa aaactgtcgt cgtgggcccc gcaatagatt ccctcctgcc
     2221 cgttgcacac tgtaaaccaa gccatggaag caggccaagg ataggtcatt cgcagagtac
     2281 gcgaagcgaa tccgtccatc cgttccccta gtccattcgg ccaataaaga tactccttcc
     2341 cactctttcc acccaaagac cggacgccgc ccaactcggg aaaataaaac tccgtaattt
     2401 ccagctctgc acgattctcc actgtggcgg cccaactcag ttcatcctcg tgcaattgga
     2461 tgctaaactg aatggtcact ggaaatgtct cctgcctgaa gcgcaaccgg tccacccgaa
     2521 ggaccaaccg attctgagcc ggcttctcga tctgatactg ttgggcctcg gccaagacca
     2581 cattctccca acactctccc cgtcgaaaca cgatgtgcca aaatccggga gcttggccgc
     2641 tgacataatt gacactcgca tccacctgct tgttgcgaag ttcacgtacc gtgccgtttt
     2701 cgtcgatcac aagcagaagc ttctcgtttt cgagccgata ctcttgggcc gacggaagct
     2761 ccaaatcgtg ggtcaggggt tggttcgatt ctggcaggga gagccgacca cgcagagtac
     2821 gataccctac gcaaaaactt tgccaaaggt attccccggg ggaaagggag gcaattcgat
     2881 agtgtcccgt tggatcggta atagacgatc gctcgatccc acgaattgtg tccaccaagt
     2941 aaagccgggc cccggcgatc ggcttgccct gaggatcagt aacttttccg accaactctc
     3001 cggcttccgc ctcggcaaaa ccaacgcaca tttgcagcca aagcactccg gcgaggagtc
     3061 cacgagccca tctcgtgtgt ttttcatacc tgtttttcat gccgacctcc acacagaggt
     3121 ttggaaccaa ggtttcctcc aaaagtcctg gaaaacaaaa tcggtcctgg ccgggagcat
     3181 agcgccccag caggggggcc gaaaactgac ctggaaatag caacaggctt ggctctaagg
     3241 tagacggtta ttgtaaagcc acaacccacc ctggagacca agccacggat ggtagtatcc
     3301 acctttcggc cgaggatcgc aacaccgcac tccacatttt tcgccaggcc aagcaggcgc
     3361 ggcgggcgtt catgttgttg cttttggacg ccgggttctt ctatcgggag attctcgctt
     3421 gtggcctaca ccagcccctt cttggtccgg acggtcaata aggattccaa ggccggcgga
     3481 ctggcccgcg cgagctctcg ctacacaggg ctatcgaatg ttgcattcta tcatcatgct
     3541 atgccgaggg ccttcatggc cgcagtcata ggatccgaga agaagattgg gtcaacgcgt
     3601 tccacgatgt caccgggtac ctctaagaag tttcgcttgt tctccaacgg gatgagtgcg
     3661 cgacgagctc cattgtccat tcccacctga agagcttcca caagcgaccg cagcgacttg
     3721 atgttcccct gaatgctgag gtcacccaga atcagcagtc ccgccagcac cgactgcttc
     3781 ttgagggccg agtacacagc cacaatgaac ccaatccctg cctcgcatgg aacatgattg
     3841 ccgagtaggt cgatgccctc gacgtggaaa tcggtggagt ccagttgctg ctccaggcct
     3901 atcttgcttt tctgcgcgat cagatacgca aaggctcgct ggatcgattc cctcatcgtg
     3961 ccgtcgatgc ccccagcaac tcgaagcttc cccgtgccag atgaacagct cacctcgaga
     4021 cggtacaaac cgactcttcc ctggtcgtcc acggaggctg tgtaaaccga gccaggagcg
     4081 agaggatcag gcgaaataat gtcctgtccg ccctgctccg gtacaccgac gaatcgctcc
     4141 tcccgcgtct cgttgtccat gtaggagaac gacgtctggt ggtactcaaa tgccccgagc
     4201 ttcttgagct gttccttgac gcgacgcctt ccctccatgg ccagttcaag gagctcagcc
     4261 agctcattcc gtgaaaactc gccgtggggg tagatgatct ttatcaatcc cgacacagtc
     4321 tttcgcaccg ccttgacgtc ccgtgcgttc aggtgtgaac ccagggagaa gaaccggtcg
     4381 atggcctccg tgaagttgtg cttgcgcagt tctcgaaggg cctcggcgaa gtagtcaacg
     4441 actaatccat agtggtccgt aaagaactcc actcgcatct tcggaatttc ccacccaggt
     4501 acgtagaagt gcaaccgatc caggaaggcc atgtcctcac gaatcacggc cggcatcggc
     4561 tggaacagat gcgaagagcg gaccatgaca tctaccgact gattggtatt tccaaacatc
     4621 gctacggagg ccatgcccgt caacgcgtcc ttacctcggg caaacgtccc ggactcacag
     4681 taggttttga gggtcgttac cacctccttg ggcattttct ggaggtcagc cacctcgtca
     4741 aaggcaatgg catcccaaat ccccacgagg cccatcttgc cagtggccat gttgtagaac
     4801 aggttcgcca cagtcgttgg acccgtgagg agaatggtgt acggtgaaag ttcctggtag
     4861 gcatagctct tccccgttcc acgggggcct agctcaacga ggttgtagtt atgctcgcaa
     4921 agcgggatca tccgcaccaa gagtagcatc ttcaggcggc ggtcgaaatg gctcggctcc
     4981 aggccaatgg tgcggagcag caggtccaac cactcgtccg tagagaactc gcggcgacat
     5041 gcgcagtact ccgcgaggtc aaacgtggcg atttggatcg gcttcagcga atcaatccag
     5101 aaaggacttc tcttgccctt tgcctcttca tcatactggt gccttatctc aacctgggcc
     5161 caaatgccgc ccatgagcag ccgatcatac tcacgcacaa aatgttccgg gacatgcacg
     5221 tacttgtggc cgaagttgac cagctcggcc cagtgcttgt cgtcgtcaga caaataacgg
     5281 accttcacct tgtcaatcag cgtgtgcctg cccttgtcct tgaccatcga ttggactttg
     5341 ttggcctcat cgggccgcac gaagttcgat gcgatgatcg agttgacaac gcggagtcct
     5401 gcctcgatgg cggcagcgtc gtcggttgcg cagtacttgc ccagtaggta ctccagcaca
     5461 aagacgggca cgtttgcgcc gaccttgacc ttgcggacaa ggtcctttcg cactacctta
     5521 ccagcaaaaa cgcttacagc cttgcggtcg agttcgggca tcgtcgtacc ctcacatggc
     5581 aatggccact tcgatcttct ccaacttaga cagttcggct ccggtcgctg cgtcaaacaa
     5641 atacaatccg acggtcttct ggtccggttc ctccacaatc atcaaggtaa ccgtattggg
     5701 gacgatcttc ttggggtccg catcgtctct cttgagcgcc acgtcaccgg tcgattcctc
     5761 aaacccgtag gacgcactta ccgcccgcga gatggccctg cccttcgccc gcagctcgac
     5821 gcggactttc ggcggctcga cgtcgaacaa cccttggctc gtgcctgtca cctgtacgga
     5881 gaagaaccgc gtcgtgagtt tttggctgcc gggcagaagc tgccagataa tcgccgtggg
     5941 tcctgtcacg gccgtcttgg ccaacggctt caagacaaga accgggatca gtgcttcctg
     6001 gggagaaagc ccgccgtgga agtacgctct ggctccgccc ttgcacttga agcacgcgaa
     6061 cgttaagggt gttgccacgt caaagtcgcc ctccatgccc accgcagtta gcggcattcg
     6121 cagcgtggcg tcattggcct ggccgccacg gcccacccac acgcggcggt gaaggtccgc
     6181 ggtctctccg cccggcgctt ctaccttcat gtcttcgctc aattcctcag caaagaggtg
     6241 cccgtggtcc gacgccaaga caatcctctc cacgcccagt tccgccagga cgcgtatccc
     6301 gcgccgcagg tcgtgcagca cgccgtccat ctgccgtcgg gcctgtgtga tgttgtcctg
     6361 ctcgcacagt tcatcaatct cctgcgaggt caccaaaacc aactgggcgt tgctgatgcc
     6421 ctcgcgtatc ttcttggtgg gctttggcag caagtcgtcc aacttggcgt cgaacaccgc
     6481 cacgcctgca cggtccttca ggaacgctac gcggtccttc cggtccttga caacgctccc
     6541 acctatctcc agcgccaacc ttccgctgcc agccgtcacc accttggcgg cctcgtgtgc
     6601 gccgggaagg agcgccgcca tgccgatctc cgtcagggtg ggtgcggccg caagagcagg
     6661 ctggacttcg acctcgaagt catcccccat cagtcgggca agctccctgc ccatctcaaa
     6721 ccgcagcgcg tccacccaca cgtaggcgac tttctcgttg cccagtgcgg gcttcacgta
     6781 cttctcgaac acttcgcgct ggcgaatgac ccccttgtcc gggaatgtct cccgcaaccg
     6841 tgtcacgaag atccgtgcaa gctcggagcc aacggccacg tatctctgcc tggcccggat
     6901 aatcagtttc tccaggtttt catcgtctct accgtgcggc tcgaagttgt accaccggct
     6961 ttccatatga cggtggcagg tgtccaggtg gcaccacggc tgggacccct ccgcgtattc
     7021 cttgatcagg cccgccaacg acgccggggc cttcttcagg gcctgctcga cgcgctgggc
     7081 ctccagcaag acctcggcgg ccaaggctat caacgcccag cgggcctgga attgcggctc
     7141 ggcctcgcac cagaattgct tttgccgggc ctcggccagt gtcaacacct ccccgtccgt
     7201 gttctccagc aggcgctttt cagcgtactg caatagcgcc tcctcgatag ccggaaatgt
     7261 ctccagctcc caaatcgccc tcgggtcgaa ctgcgtcggg tcaagcgcga actcaccttc
     7321 gacacggcgg gcagccgtca cgtagctttc gcgaagatcc cagcgcagtc gccacgcctt
     7381 ggccaagtcc acgcaggcct ttctaccttc cgacgtggaa gctaccggca ccgaggccaa
     7441 ccgtgagggc aacgcctgcc caaggccgac caccagctcc gtcagaagga catggcggga
     7501 gaggaggcag cggatgtcat ccaagctggc atggtcgaag gtcgtcaaat cgaactcctg
     7561 gcgtagcaat ttcctcaatt cttcacccgc ctccttcttg ttgatctcct cgtcgtgcga
     7621 gcggtgggtt agaaacgcca gggcgatttc ctgcgggttg cccgtgccaa agatgagcgt
     7681 caacaaaccc ttggacacct cgccaccttt ggcagccagg gcatcgaggt cggccagtgt
     7741 caacttgccc gcctcgactt gtttctcgat ctcggcggcg gcctcatcgc tcagaactcc
     7801 tcgcagcgcc ttgcgggcca cgactgccag ccgcgtgttg cgggccggcg gctgctggcc
     7861 cggctgcatg accacgcccg ccacctccag ctcaattagg gcatggtgtg tcttgtcctg
     7921 cgccaacggc acatagacca ccagccgcgg cggctcttcg ccgtccataa gtcccttttg
     7981 gtcgatccgc cagcgcaggt cgaagaaact gccctcatat cgcaggacgg tcgtgcccgg
     8041 cagttccagc acttcgaccg catcggcata cacgccgtcc gggtcgtacc agaccaccag
     8101 gccgtggtcc tcgacctgct tgcggaccaa gcccaagaga tattcggtta ccaaaccggg
     8161 cttcatcgac ttactcgctt tgtctccggt ccgcatggtt attctgcctc gtcatcggcg
     8221 gattcctctt caagttcctt ttgatcctcg tccgggtttt cggccctgag ggctgaacgg
     8281 ctggtcttgg gcggcggggc ttcgcacaga tgctctaggc cgtgcgcgat ggcaagggaa
     8341 cggtcggttc ggcacttctc cttcacccgc tccggccata gctgcatggc aatgtgcgcc
     8401 cagtcgtact taccctcctg gagcgcctcc caatatttct tgggctcctg ccagggcacc
     8461 agctcccaca gcggggcgat gttcaggatc acaccgtcat tcacatccgg cgtgatgtgc
     8521 aaatcggccg cccggcggag cttgtcgcgg aagtcctgga gttccgccac gaactgctcc
     8581 tggcggtcga tctccttctc gatttgcttg gcctcgcggc cggacttgcc cgctgcctcc
     8641 ctgcgggcgc gaagcgtgct cagccggtcc tcctccaggc gaatcttagg ctcgacgtaa
     8701 ttcagcagcg ccttgaacag gatgtctttg tccaggcggt ggtagtagag ccacaggccg
     8761 tagttgcctt tggctgagcg caggtaccag tagataggcc gtttgcggta cttctggata
     8821 tgctccttga agaatttatc ggcaaggtaa tcgcgcagcg ttgtcactaa ttccccatgg
     8881 ttcccaagtg cctggcggat cagggattcg gctttagcct cgccgaagat cacctccagg
     8941 gcagcaaaga cctttttggc caggtcgtcg gaatgggtcg agtccaccac cagcaggccg
     9001 tccgggtcgc tcagctcctg caatttgccg gcggttgccg tgtcaaagcg tcctcggccc
     9061 aacccttttt ccttgccggg ttcaaattgc cccatcacga tgccgacggc gtagctgatc
     9121 cacgccttgg cgaggtcttc ctcagaccag aaggttttag ttgtttcggt ctcttccgaa
     9181 tcgttatccc ctggctcttc ctcctc
//
LOCUS       DAOSUT010000158         9182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4022_length_9182_cov_9.481757, whole genome shotgun sequence.
ACCESSION   DAOSUT010000158 DAOSUT010000000
VERSION     DAOSUT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_9182_cov_9.481757"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..353)
                     /locus_tag="PLD05_12740"
     CDS             complement(108..353)
                     /locus_tag="PLD05_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOP78356.1"
                     /translation="MNTPEPLAYRLKDAARILGLSTRTVWQLAHDGQIPCVRVGTGRR
                     KTLLFPADALKAWLLERAKEDRAGDPGNTTEDGLNDR"
     gene            complement(626..1597)
                     /locus_tag="PLD05_12745"
     CDS             complement(626..1597)
                     /locus_tag="PLD05_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78357.1"
                     /translation="MSIKLDFGPLDAAVQEFSEAAEAFEKLWMEDDRRPACVKPKPKV
                     SDKVVRQAIVRLLKAARHLLDAVEAMPPNVRQALVHPPPARVGVCCPPLPLPQQAKAV
                     IAFAETWLPKFTDPRFWVNADFEYDFYFYVGQLLLTVWYELTKTLECYQRLKEQIEVD
                     VLLDRADTWLGNVALQDAERAAPVADETTNHDTRPAEVKESVPHGTRQGGVGRSKRST
                     EKGEARLKITAALFHHHQYDGDSVLNDDPIGVNELHRLAGVSKGMVSTFFKKEFCPQN
                     QSDGHARYRALCRDRRRLIAWLKTLDPNEKIRGHQLLNSDIPSRPED"
     gene            complement(2047..4881)
                     /locus_tag="PLD05_12750"
     CDS             complement(2047..4881)
                     /locus_tag="PLD05_12750"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOP78358.1"
                     /translation="MSRTTIDRLIINSPYEEPKRHWRYDRETRTFELVESRRPAGYVV
                     ATPGSKSFDDPGIFVEIPLVNKIRPRVKQWREAGYPGVTSVTKRLLEHWHDPERSHRF
                     FFCQLEAIETLIWLIEAPAAERVGIEIPGDGGAFTRQCCKMATGSGKTIVMAMAIAWH
                     ILNKVSNPQDARFSKNVLVVAPGLTVKKRLQVLELTADGNYYEAFDIVPSGLLDKLRQ
                     GKVLVRNWHALAWETEEEIKKRKSVDKRGVKSDEAYTRDVLGEMANARNILVINDEAH
                     HAWRVNWEAEGKYLRQRDLKDSAEEATVWIGGLDRLHRARGILKCYDFSATPFTPSGK
                     RTSEEALFGWIVSDFGLNDAIESGLVKTPRVVVRDDAVPDAKTYKSRLYHIYNDPEVK
                     NDLNRKAEPEERLPDLVLNAYYLLGYDWRETLKAWQAAGHLTPPVMITVCNRTETAAR
                     VKHAFATKQIHIDELCDTERILHIDSKVLAEAEQKEEAFTPVRAVGESESDDTEEEDT
                     PAERKLTKAEQAELLRQMVDTVGKKGQLGEKIQHVISVGMLSEGWDAKTVTHIMGLRA
                     FTSQLLCEQVVGRGLRRTSYEVNPETGLFEPEYVNIFGVPFTFLPHEGGKDGPPPPPT
                     PKTAVEPDPAKAQYEIRWPNVVRIECVLQPKLEVDWTNVHPLELDAARTMRVAELAPI
                     IEGKPDVTKIERIELERLAREFRTQRIIFETARDVFDRIKPSWRGTREVLLAQLVKIV
                     ERFIASDRITIKPPLFYQDELRRRLIITLNMTRVVEHVLQAVRQENSDRLVPIFDCDH
                     PIRSTAEMRTWYTGKPCERTKKSHINVCVYDSTWEASAGYVLDNSDEVAAWVKNDHLG
                     FEIFYLYQGVVRKYRPDFLVRLINGDHLVLEIKGQNTEQDEQKRKYLEEWCQAVTAHG
                     GFGRWQCDVVRSPGEIHQVLARALANARSN"
     gene            complement(4878..5222)
                     /locus_tag="PLD05_12755"
     CDS             complement(4878..5222)
                     /locus_tag="PLD05_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOP78359.1"
                     /translation="MWRDDAYLLDMLIAAREAQAFSKGLSWEQFRGSSLHQHAIAKAL
                     ENVGEAARKISEETRAAHPEIPWSRIVGLRHRIAHDYFHLDLVRIWEIVTSELPPLIA
                     TLELLVPPEEAE"
     gene            complement(5209..5526)
                     /locus_tag="PLD05_12760"
     CDS             complement(5209..5526)
                     /locus_tag="PLD05_12760"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOP78360.1"
                     /translation="MMKPQIEMPREAIVAFCRKWGIVEFALFGSALREDFRPDSDVDV
                     LVTFAPGVKRSFEEWLAMVRELEAIFGRKVDLVDRHLLEESKNYIRRRHILNHLEKLY
                     VAG"
     gene            complement(5523..5672)
                     /locus_tag="PLD05_12765"
     CDS             complement(5523..5672)
                     /locus_tag="PLD05_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78361.1"
                     /translation="MMDRATIQGLIINLPYEECRRYLMYGCETRTFDLVDDRGIESLK
                     VMEVE"
     gene            complement(5669..7378)
                     /locus_tag="PLD05_12770"
     CDS             complement(5669..7378)
                     /locus_tag="PLD05_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP78362.1"
                     /translation="MLTKLTICNFKLFDEVEIELGQRVVFIGPNNKGKTSALQALALW
                     DVGVKRWLEKRGGGAVPEKRPGVTINRRDLFAVPVPASNLLWRDLRVRKGSRADGRVR
                     TQNVLIEIIVEGVKGEPWRCGLEFDYANEESFYCRPLRIADGQRSEVPSHLADLRLAY
                     LPPMSGLSPREDRLELGTINVRLGEGRTAEVLRNLCWQVLQLENGKEQWQGIVQQIES
                     LFGVRLNEPRYIKERGEIIMDYRTREGITLDLSASGRGQQQTLLLLSHMALNPGAVLL
                     LDEPDAHLEILRQRQIYQVLTDQAERTGSQIIAASHSEVILNEAADRDIVIAFVGQPH
                     RINDKGSQVLKALRDIGWEHYYLAEEKGWVLYLEGATDLAMLRAFAKALNHKAQQHLE
                     RPFVHYVANQPRKAQEHFHALREAKKDLVGIAIYDRLDSEPPSDPYLVQRMWSRRELE
                     NYLCQPETLLRFAEEQGRTHHGELFGAAWRDDMDKAIKEIESALRTLGKDPWSKDIKA
                     SEELLDPLFKKFYEMRRLPNLMRKTDYHMLAPYVPVDEIDSEIAQVLDAIVTVASKAQ
                     GVA"
     gene            complement(7383..>9182)
                     /locus_tag="PLD05_12775"
     CDS             complement(7383..>9182)
                     /locus_tag="PLD05_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78363.1"
                     /translation="GVGSGFKYKTVPHVTLKSIANNPEIDGIYQRMHPAIEQAVAALN
                     AALKGKPIRFKVTQGGRAGHFVDFSAPDSETFTMPAGQVVKVNELVEWEVPFEFPADW
                     PEAARQPFERFHAARRAMQKVMDEAIARHAPQETLYDQPLVDRKKVRVTGPFTVEAVP
                     APVVKSVDEILEPPPQAADASIARSGETLRQAEWRDELLRTGIRGKGGQYIRFSRLEP
                     LPGCRWLHAEGETKEDNPQRVVVSFGPEHAPLEQRQVEHAWEEARTLDPKPKLLIFAA
                     FQFDPEAAKDIDELKPEKVGMQLLKVQMNADLLTDDLKKKRASNESFWLIGQPDVEVV
                     SEQWIVVSGQGSGTGGKEALAPYDVVAKLSGFDRLAEINEIGRDNLSYLLAIAQRRDL
                     WDAAAIDASGGVHPSEHRRGAGASRDGGVSSLPGRGTRFPSRIGDAADFVRAIESVEL
                     PERAKRIERLRGNREIAERAHQIAAWLSSGHYSLTTVRYAQVTVRGFDYYNTKTGQIE
                     SGGANKIALWMLDTDYDGRSLYPRQVFFPMAGENEGWAKLARNLKAEIDEELIEAYRG
                     TVSLPFEPGEHKRIAVKIVDDRGIESLKVVSLE"
BASE COUNT         1842 a         2767 c         2500 g         2073 t
ORIGIN      
        1 ccgacaaact tggcttgcgg tcgtcgtggg ctggacaacg ggccacccag ccggcaccgg
       61 cccggcgtgg gttgcatccg tgccgctcca gtagtgcaac cacctcgtca acggtcattg
      121 aggccgtcct cggtggtgtt gcccgggtcg ccggcgcggt cctctttggc ccgttcaagg
      181 agccatgcct tcagggcgtc ggcagggaat aagagcgttt ttcgtcggcc cgtgccaaca
      241 cgtacacacg ggatttgacc atcgtgggct agttgccaca ccgtgcgggt ggaaagcccc
      301 aagattcggg ccgcgtcctt aagacggtag gctaagggtt ctggcgtgtt catcgttacg
      361 tctcctgggg tttcaggggg tttacgtctg tgggctgcac cgtgcggccc tctacatagg
      421 accgcagtgt tcaacatggg agcgtggaac actcccgacg tggcgacgta acgtctttat
      481 ctgtaagggg ttacgtggac aagaaaattg ttccggtgtt caacatttcg tattgaacga
      541 ttgaacagtg aacacggaat tgaacgattg aacagtgaac ggggaattga acgattgaat
      601 agtgaacagg gaaaacgccc gttgatcagt cttctgggcg tgaggggatg tcgctgttga
      661 gaagctgatg gccgcggatt ttctcgttgg ggtcgagcgt tttcagccac gcaattaacc
      721 tccggcgatc acggcagagg gcgcgataac gggcgtggcc atcgctctgg ttctgagggc
      781 aaaactcttt tttgaagaat gtcgaaacca tccccttgga tacgccggct aatctatgga
      841 gctcattgac gccgatgggg tcatcgttca agacgctgtc gccgtcgtac tgatgatggt
      901 ggaacaaggc cgcggtgatc ttaagtcggg cttcgccttt ttcggtactg cgcttgctcc
      961 gtcccacgcc gccttgccgc gtcccgtgcg ggacggactc cttcacctcg gctggtcgcg
     1021 tgtcgtgatt ggtggtctcg tcggcaaccg gggcggctct ttccgcgtcc tgaagtgcta
     1081 cgttccccag ccacgtatcc gcccgatcca aaagcacatc gacttcgatt tgctctttga
     1141 gtcgctgata gcactcgagc gttttcgtca gttcgtacca aacagtcagc agcaactgcc
     1201 ccacgtagaa gtagaaatca tactcgaaat ctgcgttcac ccaaaagcgc gggtcggtaa
     1261 acttgggaag ccatgtttcg gcgaaagcga tgactgcttt cgcctgctga ggtagcggta
     1321 atggtgggca gcaaacccct actcgtgcag gtggcgggtg caccagggcc tgacgaacgt
     1381 ttggcggcat cgcttcgacg gcatcgagca ggtgacgggc ggctttcagc aagcggacaa
     1441 ttgcctgtcg caccaccttg tcactgacct ttggcttggg tttcacgcac gcgggccgcc
     1501 ggtcatcttc catccaaagc ttctcaaagg cttccgcggc ttcggaaaac tcctggactg
     1561 ccgcgtccaa agggccaaaa tcaagtttga tagacacggg tctcgctccg cgatgcagat
     1621 gcggccaaaa aaccggcggg cagcgttgcc cttacgtagt gttcaggatg cattccgtag
     1681 tgtgcgatta ggtgccgatt atagcacgct gacacgctgc ccctcaaagg ccgtcgccgc
     1741 gtcaggttgg acaacccatg tccaacctgg cccccgtggt tctgcggaat tgccttcgca
     1801 agctttgccg cttgccggtc acactgcctt gggaacgcct tgcgaagatt gcccgccgga
     1861 gcaaatcccc gttcgtttcg gcgtcaccat gccagtaaac cgccattcgg gaagcggctt
     1921 cgagcaattg agcagctcgc ttggcttcgc gtaccccgaa tgtcgcggaa gtgacgcgga
     1981 ccggtcaatg accttccaag cgccggcgtg agcttcactg aggatcagtt cgatcttgcg
     2041 ttagaatcag ttcgatctcg cgttagccag ggcacgcgca agcacctggt gtatttcacc
     2101 aggagacctc accacatcac attgccaccg gccaaagccg ccatgggcag tgacggcctg
     2161 gcaccattcc tccagatact tgcgcttctg ctcatcctgt tccgtatttt ggcctttaat
     2221 ttccaagacg agatgatcgc cgtttataag gcggaccaaa aaatcaggac ggtactttcg
     2281 caccacccct tggtaaagat aaaaaatttc aaagccgagg tggtcgtttt tcacccaggc
     2341 ggcgacctca tcgctgttat caagcacata acctgccgag gcttcccagg tgctgtcata
     2401 cacgcagacg ttgatgtgtg atttcttcgt gcgttcgcag ggcttgcccg tgtaccaggt
     2461 gcgcatctca gcagtggagc gaatagggtg gtcgcagtcg aagataggca ccaagcgatc
     2521 gctattctcc tgcctcacag cctgcaagac gtgctcgacg acacgggtca tgttcaacgt
     2581 aataataagc cgccggcgca gttcatcttg gtaaaacaag ggcggcttaa tcgtgatccg
     2641 gtcagaagcg atgaatcgct cgacgatctt caccaactgg gccagcagaa cctcgcgggt
     2701 gccccgccaa gagggtttga tccggtcgaa cacgtcgcgt gcggtctcga agatgattcg
     2761 ctgggtgcgg aactcgcggg ccagccgctc cagctcaatg cgttcgatct tggtaacgtc
     2821 gggcttcccc tcgataatag gtgccagttc ggctacccgc atcgttcggg cggcatccag
     2881 ttccaagggg tggacattgg tccaatccac ttctaacttc ggctggagca cgcactcgat
     2941 ccgcactacg ttaggccagc gaatctcata ctgggcctta gctgggtcag gctccacggc
     3001 cgtcttcggt gtcggcggcg gtggcgggcc atcctttcct ccctcgtgcg gtagaaatgt
     3061 aaagggcacg ccaaagatgt tgacgtattc cggctcgaac agacccgtct ccggattgac
     3121 ctcgtaggag gtacgccgca acccgcgccc caccacctgc tcgcagagaa gctgagacgt
     3181 aaaggctctc aaccccataa tgtgagttac cgtcttggcg tcccagcctt cggaaagcat
     3241 gccgaccgag atgacatgct gaatcttctc gccaagttgc ccctttttac cgacggtatc
     3301 caccatctgg cggaggagtt cggcttgctc agccttggtc agcttgcgct ctgccggggt
     3361 gtcttcctct tctgtatcat cggactcgct ctccccaacc gcgcggacag gggtaaaggc
     3421 ttcctccttc tgttccgcct cggccagcac cttggagtca atatgcaaga tgcgctccgt
     3481 atcgcatagc tcgtcgatgt gaatctgttt ggtcgcgaag gcgtgcttta cccgggctgc
     3541 cgtctccgtg cgattgcaga cggtgatcat caccggcggg gtaagatggc cggccgcctg
     3601 ccatgccttc agcgtctccc gccaatcgta accaagcaga tagtaggcgt tcagcacaag
     3661 gtcgggcagt cgttcctccg gctccgcttt tcggttcagg tcgtttttca cctccgggtc
     3721 gttgtagata tgatagaggc gcgatttata ggtcttggca tctggcacag catcatcgcg
     3781 caccaccact cgtggcgtct taaccaggcc cgactcgata gcatcgttca acccaaagtc
     3841 actaacgatc cagccaaaga gggcttcttc actggtccgc tttcccgacg gagtaaatgg
     3901 agtggctgag aagtcgtagc atttgagaat gccacgcgcg cggtgcaacc ggtccaggcc
     3961 accgatccag accgttgcct cctcggcgct atccttgaga tcacgctgcc gcaaatactt
     4021 gccttcagcc tcccagttta cgcgccaggc atggtgtgcc tcatcattga tcaccagaat
     4081 attgcgggca ttggccattt cgccgagcac gtcacgcgtg taggcttcgt cactcttgac
     4141 gccgcgcttg tccacgcttt tgcgcttctt gatctcttct tcggtttccc atgccaaggc
     4201 gtgccagtta cggaccagga ccttgccctg ccgtaattta tcgagcaggc cagaggggac
     4261 aatgtcgaag gcctcgtaat agttgccgtc ggcggtcaat tcgagaacct gaagccgctt
     4321 cttcaccgtc aagcccggcg caacaacgag gacgttcttc gaaaagcggg catcctgcgg
     4381 gtttgatacc ttgttaagga tatgccaggc aatggccatg gccatcacga tggtcttccc
     4441 cgagccggtg gccattttgc agcattgccg cgtaaacgcc ccgccatcgc cgggaatttc
     4501 gatgcccacc cgctcggcag ccggggcctc gatgagccaa atcagcgtct cgatggcttc
     4561 caattggcag aagaaaaatc ggtggctgcg ttccgggtca tgccagtgtt ccagaaggcg
     4621 tttggtaacg ctcgttaccc cgggataacc tgcctcgcgc cattgtttta cccgcggccg
     4681 aatcttgttg accagcggaa tttccacgaa gatgccggga tcgtcgaaag attttgaccc
     4741 cggtgtggcc actacatagc ctgccgggcg acgactctcc actagttcga acgtgcgcgt
     4801 ctcgcgatcg taacgccaat ggcgtttcgg ctcctcgtag ggtgagttga taatcagccg
     4861 gtcaatggtc gtgcgactca ttctgcctcc tcagggggta caaggagttc caaagtggcg
     4921 atcaaaggtg gaagctcgct cgtcacgatc tcccatattc tcaccaaatc aaggtggaaa
     4981 taatcgtgag caatgcgatg ccgcagaccg acgatccgtg accaggggat ttcgggatga
     5041 gcagcccggg tttcttcaga aatcttgcga gccgcttcgc ccacgttttc cagtgccttg
     5101 gcgatggcat gctgatgaag cgagctgccc cgaaactgtt cccatgaaag ccccttggaa
     5161 aacgcttggg cctctcgggc agcaattaac atgtccaaca aatacgcgtc atcccgccac
     5221 ataaagcttc tctaagtgat tcaaaatgtg cctgcgacgg atatagtttt tgctttcttc
     5281 taggagatgg cgatcaacaa gatcgacttt tcgcccgaaa atggcctcaa gttcgcggac
     5341 catggcaagc cattcctcaa aagaccgctt tacgccaggg gcaaaggtaa ccagcacatc
     5401 cacatcgctg tctggccgaa agtcctcccg aagtgcggaa ccaaagagag caaactcgac
     5461 gatcccccat ttccggcaaa aggccacaat cgcttcgcgc ggcatctcta tttgcggctt
     5521 catcattcca cctccatcac cttcaagctc tcgattcccc ggtcatccac caggtcgaag
     5581 gtgcgcgtct cgcacccata catcaagtac cgtcggcatt cctcgtaagg caagttgata
     5641 atgagaccct gaatggtagc cctgtccatc atgccactcc ttgcgctttg ctggctactg
     5701 tgacgatggc gtcaagcact tgggcgattt ccgagtcgat ctcgtccact gggacgtatg
     5761 gcgccagcat gtggtaatcc gttttacgca tcagattggg aagtctgcgc atctcgtaga
     5821 actttttgaa cagtggatct agtaattctt cgctcgcttt gatgtctttg ctccacggat
     5881 cctttcccaa agtccgcaag gcactctcaa tttctttgat ggccttgtcc atatcatcgc
     5941 gccaagccgc accaaagagt tctccatgat gtgtgcggcc ttgttcctcg gcaaagcgga
     6001 gcaacgtttc aggttggcac agatagttct ccagctcgcg tcgcgaccac atccgttgta
     6061 ccagatatgg atcgctaggt ggttccgagt ctaatcggtc gtaaatggca atgccaacga
     6121 ggtccttttt tgcctcccgc aaagcatgaa aatgctcctg cgctttgcgc ggctggttgg
     6181 caacatagtg cacaaatggc ctctcaaggt gttgctgtgc cttgtggttc aaagccttgg
     6241 caaaggcacg cagcatggca agatcggttg caccctccaa gtacaatacc caaccctttt
     6301 cctcggcgag gtagtaatgc tcccatccaa tgtcgcgtag cgccttgagt acctggctgc
     6361 ccttgtcatt gatgcggtgc ggctggccga cgaaggcaat cacgatgtcg cggtcggccg
     6421 cttcgttcag gatgacctcc gaatgactgg cagcgataat ctggctaccc gtcctctctg
     6481 cctggtcggt cagcacctgg taaatctgtc gttgccgcaa gatctccagg tgtgcatcgg
     6541 gttcgtcaag gagcagcact gccccggggt tgagggccat gtgcgacagc agaagcagtg
     6601 tttgctgctg gccgcgcccg ctcgccgata gatcgagtgt aatcccctcg cgcgtgcgat
     6661 agtccataat gatctcgccc cgctccttga tgtagcgcgg ctcattgagc cgaacaccga
     6721 acagcgactc gatttgctgc acgattcctt gccactgctc tttgccattt tccaattgca
     6781 acacctgcca gcacaagttg cgcagcacct ccgccgtgcg cccttctcca aggcgcacgt
     6841 tgattgtgcc cagttccaat cgatcctcgc gtggtgaaag accggacatc ggcggcaggt
     6901 aggccaagcg taaatcggca agatgcgaag gcacttccga tcgctgaccg tcggcaatgc
     6961 gcaggggccg gcagtaaaac gattcctcgt tggcgtagtc gaactccagc ccacatctcc
     7021 acggctcccc tttaacacct tcgacgatga tttcaatgag cacgttctgc gtacggaccc
     7081 tgccatcggc gcggctgcct ttgcgcacac gcagatcacg ccacagtaga ttggacgcag
     7141 gcaccggcac agcaaacagg tcacgccggt tgatggtcac gcccggccgc ttctcgggca
     7201 ccgcaccccc gcctcgcttc tccagccagc gcttgacgcc cacatcccac aaggcgagcg
     7261 cctgcaaggc agaagtcttg cccttgttgt taggcccgat gaagacgaca cgctgaccga
     7321 gttcgatttc gacttcgtcg aacagcttaa aattgcagat tgtgagcttg gtcagcatgg
     7381 tatcactcca gacttacgac cttgaggctc tcgattcccc ggtcatccac aatcttgacg
     7441 gcgatgcgct tatgttcccc cggctcaaaa ggtagcgaca cggtgccgcg gtaagcctcg
     7501 atcagctcct catcaatctc cgctttaagg ttacgagcga gctttgccca gccttcattc
     7561 tcgcccgcca tcggaaagaa aacttggcgc gggtaaagac tccgcccgtc gtagtcggta
     7621 tcgagcatcc agagagcgat cttattagcc ccacccgact caatctggcc cgtcttggtg
     7681 ttgtagtaat cgaagccgcg aacagtaact tgagcgtagc ggacagtggt tagggaatag
     7741 tggccagaac tcaaccacgc agcgatttga tgagcccgtt cagcaatttc ccgatttccg
     7801 cgcaatcgct caatgcgctt tgcacgttca ggcaattcaa cagattcaat cgctcgcaca
     7861 aagtcagcag cgtctccaat tcggctaggg aaccgcgtgc cacgcccagg aagtgaagaa
     7921 actcccccgt cccgtgacgc gcctgcccct cggcgatgtt cgctgggatg gacaccgccg
     7981 ctcgcgtcaa ttgctgccgc atcccatagg tctcttctct gggcaattgc gagcaaatac
     8041 gatagattgt ctcggccaat ctcattgatt tctgccagac gatcaaatcc tgataacttt
     8101 gcaacgacgt catagggtgc caacgcctcc tttccaccag tgcctgaccc ctgtccactg
     8161 accacgatcc actgctcact gaccacttcc acatccggct gcccaataag ccagaaagac
     8221 tcgttgctgg cccgcttttt cttgaggtca tcggtcaaaa gatcggcgtt catctgcact
     8281 ttgagcagtt gcattcccac cttttcaggt ttgagttcgt caatgtcctt agctgcctcc
     8341 ggatcgaact ggaaggcggc aaagatgagc aatttaggct tggggtcaag cgtccgtgct
     8401 tcctcccacg catgttccac ctggcgctgt tccaatggcg catgctccgg gccaaaggag
     8461 accaccacac gctgcgggtt gtcctccttc gtctcgccct cggcatgcag ccagcggcaa
     8521 ccgggcagcg gctcaaggcg gctgaagcgg atgtattgcc cgcctttccc ccggatgccc
     8581 gttctcagca gttcatcgcg ccattcggcc tgacgcagcg tttcgccaga ccgggcaatc
     8641 gaggcatcgg ccgcctgggg tggaggctcc agaatctcat ccaccgattt caccaccggc
     8701 gctggtaccg cttcaaccgt gaaggggccg gtcacccgca ccttcttgcg gtccaccaac
     8761 ggctggtcgt agagcgtctc ctgcggcgca tggcgggcaa tggcctcatc catcaccttt
     8821 tgcatcgccc ggcgggcagc gtggaatcgt tcaaagggtt ggcgcgccgc ctcgggccaa
     8881 tcggcgggga attcaaacgg cacttcccat tccaccagtt cgttgacctt gaccacctga
     8941 cctgcgggca tggtgaaggt ctcgctatcg ggggccgaaa agtccacaaa atgccccgcc
     9001 cgaccgccct gggtgacctt gaagcggata ggtttgcctt tcagtgccgc gtttaacgcc
     9061 gccacggcct gctcgatcgc cgggtgcatg cgctggtaga tgccgtcgat ctccgggtta
     9121 ttggcgatcg acttgagcgt cacgtgcggc acagttttgt acttaaagcc gctgcccacg
     9181 cc
//
LOCUS       DAOSUT010000159         9110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4062_length_9110_cov_8.142573, whole genome shotgun sequence.
ACCESSION   DAOSUT010000159 DAOSUT010000000
VERSION     DAOSUT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4062_length_9110_cov_8.142573"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLD05_12780"
     CDS             complement(<1..397)
                     /locus_tag="PLD05_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOP78364.1"
                     /translation="MTSGKTLRLGTRGSLLARWQAEWVASQLRAHGVEVEIVPVLTSG
                     DRNQHWAVESLGGAGVFTKEIQLALLANEVDLAVHSMKDLPTQPVPGLAIAAIPKRGP
                     VGDVLISRTAQTFEQLPSGAIIGTGSRRRA"
     gene            complement(687..1628)
                     /locus_tag="PLD05_12785"
     CDS             complement(687..1628)
                     /locus_tag="PLD05_12785"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOP78365.1"
                     /translation="MPDKAFLGHEQAFSKKREPGPFDPFFKAVWRGAATILPPLITFV
                     IIIWIVTTINQYIFSPLTRAVRDLIFVATADIVTSSERSPEIVRDGKTYVRFKGGKYV
                     PIEVVNYIREEIGEDKLPATATEVYQLFIEERYLKPHLVLPFLIAILVLLLYLVGKIV
                     TVRIGRITWAQIESLFSSLPFVRSVYGAVKQVSDFILAQRSMEFSRVVAVEWPRRGMW
                     MIGLVTSESFSQLQTKTGQEMLSVFIPTSPAPMTGFTVNVPISDTFDLGISIDQAIQF
                     IVSCGVVVPIPEAHGVTLDQQKHPTASPSNPADSDSE"
     gene            2280..2759
                     /locus_tag="PLD05_12790"
     CDS             2280..2759
                     /locus_tag="PLD05_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOP78366.1"
                     /translation="MKFRDFVVKDAIVTDLDPYLTKEEIIRKLCESLVTAGALKREQL
                     DDIVKALIKREELGTTGIGMGVAVPHTKHPSVDRLVATIGISPEGVDFDALDGERVQS
                     LFLLVSPTDLPGDHLRALEYITRQLHNSTFARFLKQAKTIDEVWTLLEEADNGKYTH"
     gene            2952..3308
                     /locus_tag="PLD05_12795"
     CDS             2952..3308
                     /locus_tag="PLD05_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOP78367.1"
                     /translation="MSLTSGNSAVKRRWVTIRNRAGLHTRAATLVRETVLKHRSRVFL
                     IKPENNGECLDGAPRADATSVIEMLSLCALEGTPLVLEAEGEDADRVLDELQNLVNCK
                     FWEDDFAPESEEANHA"
     gene            3367..5115
                     /gene="ptsP"
                     /locus_tag="PLD05_12800"
     CDS             3367..5115
                     /gene="ptsP"
                     /locus_tag="PLD05_12800"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOP78368.1"
                     /translation="MMRFQGIAVSSGVAIGKAVVIGQDVFSIPSQYVAADAVEQEISR
                     FHQAVETAAKAIERNRDLVTQELGPKSGAIFQAHLEILRDQKIHADIERLIREKQYSP
                     ERACTEVLRRYADVFRRLPNPEFAERAYDVMDIERQLLRALLGEPRRSLSHLTSPAIL
                     LARNLTPSEVANLDRKMIKGFVTELGGPTSHTAIVAQGLGIPAVVGTGPFLAEVAGGD
                     TVIVDGDRGIVIVHPDEETLREYDQEVRSIQAFTAELEKLHDLPAVTLDGTPINIYGN
                     IEFPHEAVLCLKHGAEGIGLYRTEFLYLGRSDLPSEEDHYRAYREVIEAMGDRIVTIR
                     TFDLGADKVAAQFGFEKEPNPCLGLRSIRLALRHLELFRTQARAILRASVLGNIQVMF
                     PMIATVAELRQAKLIWADVMEDLAENGIPFRHDIPIGIMVEVPSVAIMIDRFLDEVDF
                     VSIGTNDLIQYTLAVDRTNRNVVSMYNPTDPAVLRLIAYTIQEANRKGVPVSLCGQMA
                     SHPLYTMLLIGLGLRKLSVTSSAIPEVKKVCRSVTLEQCERVARRALEMENARNIHAF
                     LREQLVKVVPAFARMI"
     gene            5603..>9110
                     /locus_tag="PLD05_12805"
     CDS             5603..>9110
                     /locus_tag="PLD05_12805"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78369.1"
                     /translation="MDEVIRQVLEKQYGLKIGDRIGRGGFAEVYRAESASGVLCAIKV
                     SLDPVNENNPAVKKELENLRLIQAVNGHPNIVSLMDYWVITGYLVTRWEFSAEGSLQD
                     LLERYREEGYPGIAREKLFRYMADAAMGIDFLNRRGIYHRDIKPQNLLLFQGRVKLAD
                     LGLAKFAGASTASHTGSGTMGYLPPEAYDARRLSETVDLYSLAATYVKLRTGREPFGD
                     YLLEVIERQKAGKPVLDGLEPDEVEAVRWALAPRPEDRPREGAVKWLRALYSRGGGRS
                     VTAEVRPRSQFSELLVDPHGRGDVRTLAEALAKLQPGGVLRLTAGTHLVDQCLEVTQA
                     ATIVGEGMERTKVVCRAAGSILRLSGQGTLHVRGICFERLGASPGNVVDVTGGEVLFA
                     ECRFENGVYREGECWGAGLVLRGETHGIVRRCVFRRNQLGIVVTDQARPVLEENLCEE
                     NGDCGIAYFGSASGTSRRNTCRGNKLHGIYAGEQSQPVLEENLCEENGNCGIAYFGSA
                     SGKAWKNTCRGNGLHGVYVSDQAQPELQENVCEQNGDSGIAYFRSASGKAWRNTCRGN
                     KRYGVYAGERAQPALGGNFCEGNAKCGIVYLDNASGKAWKNTCRGNKLHGVRVGDQAQ
                     PVLEENLCEGNEECGMVYFDSAGGKARKNTCRGNNVTGIWVDGQAQPELEENVCEGNG
                     GCGIVYIDSAGGKARKNTCRGNKLHGVEVSDQACPLLEENVCEKNQSSGIAYFGSASG
                     KAWKNTCRGNNGDGIYAGEQSQPLLEENLCERNAGCGIGYFESAAGKARKNRCRANKL
                     DGVKVRDQAQPQLKENLCEGNAKCGIAYVVSALGRAQKNTCCGNKLHGIAVSGQAQPE
                     LQENLCEGNGKCGIAYFESAGGKASKNTCRGNKLHGVYVTDQAHPVLQENVCERNGDC
                     GIVYLGSALGTAQRNICRGNGEDGIYAGEQSQPMLEENLCEGNGCGIAYFGSAGGKAW
                     KNICRGNKVHGFEVTNEAQPVLEENLCEGNGECGIAYLNSASGKAWKNTCRGNENSGI
                     WLADQAQPVLEENLCEENDCGIAYLGSAWGRAWKNTCRGNKRDGVSVGGESLPVLEEN
                     LCEKNAFGIAYCGSAAGRASKNTCRENKRHGVYVGGRAQPVLEENLCEGNGDCGIAYR
                     GSAAGKAWKNICRGNKNYDIYVG"
BASE COUNT         2088 a         2188 c         2900 g         1934 t
ORIGIN      
        1 ccgcccggcg gcgactgccc gtccctatga tcgctcccga cggaagttgt tcgaacgtct
       61 gagcggttcg ggagatgagg acatctccca ccgggccccg tttaggaata gcggcgatcg
      121 ccaatccggg gacgggttgc gtgggcaagt ccttcatgct gtggacagcc aaatctacct
      181 cgtttgcaag aagggccagc tggatttcct tggtgaaaac cccggcacca cctagcgact
      241 caacggccca gtgttgattt cgatcaccgc ttgtcaaaac aggaacgatt tcgacttcaa
      301 caccgtgcgc acgcaattgt gaggctaccc attcggcttg ccatcgagcc aagaggctcc
      361 cccgagttcc caagcggagc gttttaccgg acgtcatggt tccaaatcac aagtccaaga
      421 gacacacgga tgccaccaaa ccttcaatcg aagggaaagc atccgattat aaaccaaaga
      481 aaagtccccg ggggtatggc accaacgcct gttcaactag attgcccaaa agcatttgtg
      541 ggatcacgcg gtggttgccc gatgcgaaac ccgtggaggc ctattctcgt gcgagggcga
      601 tccactgatc gttgtgctga aacacgacgc gccggagcac cccaccgagt cacggccacg
      661 gatgctcgca cctcatgtgg aacgtcctac tcggaatcgc tatccgcggg attcgacgga
      721 gacgcggtcg ggtgcttttg ttgatcaagt gtgacaccgt gagcctcggg gatgggaaca
      781 acaaccccac aactcacgat gaattgaatc gcctgatcga tcgagatgcc cagatcgaaa
      841 gtatcactga tcggcacatt gacggtaaaa cccgtcatcg gagcaggaga agtcggaatg
      901 aagacactta gcatctcttg gccggtcttc gtctgaagct gggagaagct ctcgctcgtg
      961 acaaggccga tcatccacat tccgcggcgt ggccattcca cggccacgac ccggctaaat
     1021 tccatggatc gttgtgcaag aataaaatca ctaacttgct tgacagctcc atagacgctc
     1081 cgaacaaaag gaagtgaaga aaaaagcgac tcgatttgcg cccaggtgat ccgcccgatt
     1141 cgtaccgtga caatcttgcc aaccaggtaa agcagcaata ccaagattgc gatcaaaaat
     1201 ggcaggacaa gatgcggttt aagatatcgc tcctcaatga ataattgata gacctccgtg
     1261 gcggttgccg ggagcttgtc ctcgcctatc tcttcccgaa tatagttgac cacctctatc
     1321 ggtacatact tccccccctt gaagcggaca tatgtctttc cgtcgcggac aatctcgggg
     1381 cttctctcgg aagaggtcac aatgtcggcc gtggcgacga aaattaggtc gcgaacagcc
     1441 cttgtaaggg gcgaaaaaat gtattgattg atcgttgtga cgatccaaat aatgatcaca
     1501 aatgtgatca acggtggcaa tattgtggca gctccgcgcc aaactgcctt aaaaaaagga
     1561 tcgaacggcc ctggttcccg ttttttcgaa aacgcctgtt catgtccgag gaatgcttta
     1621 tccggcatat aaactccgaa aaaatgttgg gatggttccg tcggtacaga ttgcccgcac
     1681 agcgcgaacg tttaactaat gatgcttatc ccttatgata acgcgataca ttgtactaaa
     1741 gaatgccgaa ccggggtaaa cgagagtacg accgtcagtg tgtgatctgc tccaggccca
     1801 cgaaggccta tcggtgattg ataggctcac gtcctttatg tggacggcac cccacatcgg
     1861 tggggtatgg cactgcatcc gtggagcggc ataccattgg cgtcgaatga tgcctgggat
     1921 aaactgagag tttcggcctc cggcgggttt cttaaaaggt agaattgttg tgtgattttt
     1981 gaggccggta gttcgatgag cctatgagct tgtgggccgc ccgagtggct tggcatgtta
     2041 ccgggcaccc tgatggatag tgccgaccga acttgaagca caccatccgg cagatgtggg
     2101 aacaggtgat cgggcgattt cgagtggttt gaaagaaaac acgagcaagg ataactgaag
     2161 ttcgacaaga ggccttagcg agatgcttcg gccgatatgt ccaggtgttg tgagagccgg
     2221 cggaatatcc attgcgtgat tccgctggcc cgttggtttc aggaaaggga atttaccgca
     2281 tgaaattccg cgactttgtg gtgaaggacg cgattgtcac ggatttggat ccctatctca
     2341 ccaaggaaga gattattcgc aagctgtgcg aatcacttgt gacggctgga gcgctgaagc
     2401 gcgaacagct tgacgacatt gtaaaagcgc tcatcaagcg ggaagaactt ggcacgactg
     2461 gaatcggcat gggggtggcc gtcccccata cgaagcaccc cagcgtggac cggctcgtgg
     2521 caaccatcgg tatcagcccc gagggggtgg acttcgatgc cctggatggc gagcgcgtcc
     2581 agtcgctctt ccttttggtg tcaccaacgg accttccagg tgatcacctg agggccttgg
     2641 agtacatcac gagacagctt cacaactcca cgtttgcgcg attcttgaaa caagccaaaa
     2701 cgatcgacga ggtgtggaca ctccttgagg aagcggacaa cggcaagtac acgcactaac
     2761 tggacgtgcc tcccaccggc tccgctgcgg gacccgagaa acagtttgtg ccatccgggg
     2821 tgtgacctcg agaaaacaaa ccgctgagac ttgttggttt gatgtcctcg tgatttcacg
     2881 acatttttcc cagtgagtcg gaagttcgta gtggccgaca acgaccttgt tggcacgaat
     2941 caaaggaaga catgagtttg acttcgggaa attcagccgt aaaaaggcgt tgggttacga
     3001 ttcgtaaccg ggcggggctg cacacacggg cagctaccct ggtgcgtgag accgttctca
     3061 agcaccgcag ccgcgttttc ttaatcaaac cggaaaataa cggggagtgt ttggacggcg
     3121 caccgcgagc cgacgcgaca agcgtgatcg agatgctaag cctctgtgcc ctggaaggga
     3181 cccctcttgt tctggaggcc gaaggagaag acgcagacag ggtactcgat gaattgcaga
     3241 atctcgtgaa ttgcaagttt tgggaggacg actttgcccc agagtcggaa gaggcaaatc
     3301 atgcgtaaac ggcatgtcaa ggcggcatcg gggatttgtc ccccacggga agtctgagat
     3361 caagcaatga tgcggtttca gggtattgct gtttcttcag gggtcgccat tggcaaggcc
     3421 gttgttatcg gacaagacgt cttctccatc ccgagccaat acgtcgcggc ggacgcggtt
     3481 gagcaagaga taagccgttt ccaccaggcg gtcgagacgg cggcgaaagc catcgagcga
     3541 aaccgtgatc ttgtcacgca ggagttgggg ccgaaatcgg gggccatttt tcaggcccat
     3601 ctggaaatcc ttcgggacca aaagatccat gcagatatcg agagactgat ccgcgaaaaa
     3661 cagtattccc cggagagggc atgcaccgag gtcttacggc ggtatgcaga tgtcttccgg
     3721 cggctcccca atccggagtt tgctgaacgg gcctatgatg tgatggacat tgagcgacaa
     3781 ttgttacggg cacttttggg ggaaccacgt cggtcattgt cccatttgac gtcaccagcc
     3841 atcctcttgg cccgcaatct cacgcccagc gaggtggcca atctcgatcg gaagatgatc
     3901 aaaggtttcg ttaccgagtt gggtggccca accagccaca cagcaatcgt ggctcagggt
     3961 ctcggtatcc cagcggtcgt tgggaccggg ccattcctgg ccgaggtcgc cggtggtgac
     4021 acagtcattg tggatggtga tcgcggcatc gtcatcgttc atccagacga ggaaacgctc
     4081 cgcgaatacg atcaggaagt ccgcagcatt caggccttta ccgccgaact agaaaaactt
     4141 cacgacctcc ccgcagtcac gctcgatgga acaccgatta atatctacgg caacatcgaa
     4201 ttcccccatg aggccgtcct ttgcttgaag catggcgcag aaggtattgg gctttatcgc
     4261 acggagtttc tttatctcgg ccgatcggac cttcccagcg aggaggacca ctatcgcgct
     4321 taccgcgaag ttatcgaggc aatgggcgat cggatcgtga cgatccgcac gtttgatcta
     4381 ggggccgata aggttgctgc ccagttcggg tttgaaaagg aacctaatcc ctgccttgga
     4441 ttgcgtagta ttcgcctggc cttgcgacac ctggagctat ttcgcacaca agcccgcgct
     4501 atcttgcggg caagtgttct gggaaacatt caggtcatgt ttcccatgat tgccaccgtt
     4561 gccgaattac gacaagccaa gcttatttgg gccgacgtca tggaggattt ggccgaaaat
     4621 ggtatcccgt ttcggcatga cattccgatt gggatcatgg tggaggtgcc ttcggtggca
     4681 attatgattg accgatttct tgatgaggtg gatttcgtca gtatcggcac caacgacctc
     4741 atccaataca ccttggcggt ggatcggacg aaccgcaatg ttgtctccat gtataacccc
     4801 accgaccccg cagtgctaag gctcatcgcc tataccattc aggaggccaa ccggaagggt
     4861 gttcccgtaa gtttgtgcgg ccagatggca agtcatcctc tttatacgat gctgcttatt
     4921 ggcctggggc tgaggaagct aagcgtcaca tcgtcggcta tccccgaggt caagaaagtc
     4981 tgtcgcagcg tgacgttgga gcagtgcgag cgcgtggccc gtcgcgcttt ggaaatggag
     5041 aatgctcgca atatccacgc cttcttgcga gagcaactgg taaaagtcgt tcccgccttt
     5101 gcccggatga tttgagcgat cttgccttca tcgggaacgc catgcccgga gaaggtttat
     5161 cttgtctggc cccaattcgg tttcggtgtg ggcaaaaaac tcgctactct cagcatgctg
     5221 ttttctcgaa gccgtgggaa ttccgcggct gatcatgttt ggaagccgtt gtgcgaaacc
     5281 ctgcactgtt agttcctctg cgtgcagcca ctcgtcgctc aaaaggtgta tgatgctcgc
     5341 cggaagcgaa caatgttgaa gccgtgtggt ggtgttgcta acctaaaaaa aggtttagga
     5401 cttgactctt tgggcaggag ataggccctg attgtgccta gggatggggt gcgcgtgagg
     5461 tggagcgatt tcttttgtct gatcggactg gggttccgcc tatgcggatg ggtcgcgtac
     5521 gcagtggaga caaatgcaga aagtctatcc gtcttgcgag gtgcgctagt cgtacgttcc
     5581 tcgatgagga aggggcgcga cgatggacga agtgattcgc caggtcctgg aaaagcagta
     5641 cgggttaaaa atcggggatc gcattgggcg gggtggcttt gccgaggtgt accgggcaga
     5701 gtcggccagc ggggtcctgt gcgcgataaa ggtctcgctc gatccggtga acgagaataa
     5761 tccggcggtc aagaaggaac tggagaacct tcgactgatc caggccgtca atggccatcc
     5821 gaacatcgtc tccctgatgg actattgggt gatcaccggc tacctggtga cgcggtggga
     5881 gttttctgcg gaggggagcc ttcaggatct gcttgagcga taccgggagg agggataccc
     5941 gggcattgcg cgggagaagc tttttcgcta catggccgac gcggcgatgg ggatcgattt
     6001 cctcaatcgt cgcgggatct atcatcggga catcaagccg cagaacttgc ttttgtttca
     6061 ggggcgggtg aagttggcgg atttgggcct ggccaagttt gctggggcct cgactgcgtc
     6121 gcacaccggg tcggggacga tggggtatct tcccccggag gcctatgatg cgcgccggct
     6181 gagcgagacg gtggaccttt acagcctggc ggcaacgtat gtcaagctcc ggacggggcg
     6241 agagccgttt ggtgattatc tgctggaggt aatcgagcgt caaaaggcgg gcaagccggt
     6301 tctcgacggg ctggagcctg acgaggtgga ggctgtgcgg tgggcccttg cccccagacc
     6361 ggaagaccgt ccccgggaag gggcggtcaa gtggctgcgg gccctgtatt cccgcggagg
     6421 agggcgaagc gtaacggcgg aagtccgccc ccgaagccag ttcagcgagt tgctggttga
     6481 tccgcacggg cggggcgatg tgcgaacgct ggcagaggcc cttgcgaagc ttcagcctgg
     6541 tggggtcctc cggctgacgg cagggacaca ccttgttgat cagtgccttg aggtgacgca
     6601 ggcggcgaca attgtgggtg aggggatgga acggacaaag gttgtttgcc gtgccgccgg
     6661 gagcatcttg cggctttccg gccaagggac gttgcacgtg cgagggatct gttttgaacg
     6721 tcttggggcg tcgccgggga atgtcgtgga tgtgacgggg ggcgaggtcc tgttcgcgga
     6781 gtgtcgcttt gagaacgggg tttatcggga gggcgaatgt tggggagccg gtcttgtgct
     6841 tcggggtgaa acacatggca ttgtgcgaag atgtgtcttt cggcggaatc agcttggaat
     6901 cgtcgtaacc gaccaagcgc ggccagtgtt agaggaaaac ctgtgtgagg aaaacgggga
     6961 ttgtggaatc gcgtattttg gtagcgcttc ggggacgtcg cgtcggaaca cctgccgcgg
     7021 gaataagctg catgggatat acgctggtga acaatcgcag ccggtgttag aggaaaacct
     7081 gtgtgaggaa aacgggaatt gtggaattgc gtattttggt agcgcttcgg gtaaggcgtg
     7141 gaagaacacc tgccgcggga atgggctgca cggtgtctat gtatctgatc aagcgcagcc
     7201 ggaattgcaa gaaaacgtat gtgagcaaaa cggcgatagc ggcattgcgt acttccgcag
     7261 tgcttcgggt aaggcgtgga ggaacacgtg ccgcggaaac aagcggtatg gggtgtatgc
     7321 gggtgaacgg gcgcagccag cattaggggg aaacttttgc gagggaaacg cgaagtgtgg
     7381 gatcgtgtat cttgataacg cctcggggaa ggcatggaag aacacctgcc gcgggaataa
     7441 gctgcacggt gtccgggtgg gtgaccaggc acagcccgta ttggaggaaa acctgtgtga
     7501 ggggaacgag gaatgcggga tggtgtattt tgatagcgcg ggggggaagg cgcggaagaa
     7561 cacctgccgc gggaataacg ttaccggtat ttgggtagac ggacaggcgc agcccgagtt
     7621 ggaggaaaac gtgtgtgagg ggaacggggg gtgtgggatc gtgtatattg atagcgccgg
     7681 ggggaaggcg cggaagaaca cctgccgcgg gaataagctg cacggtgtcg aggtgagtga
     7741 ccaagcatgt cccctgttgg aagaaaacgt gtgcgaaaaa aaccagtcct ccgggattgc
     7801 ctattttggt agcgcttcgg gtaaggcatg gaagaacacc tgtcgcggga ataacggaga
     7861 tgggatatac gctggtgagc aatcgcagcc tttgttagag gaaaacctat gtgaaagaaa
     7921 cgcgggatgt gggatcggct attttgaaag cgctgccggg aaggcgcgga agaacagatg
     7981 ccgtgcaaat aagctcgatg gcgtgaaggt gcgtgatcag gcgcagcccc aattgaagga
     8041 aaatctgtgt gagggaaacg cgaaatgcgg gatcgcctat gttgttagcg cgttggggag
     8101 ggctcagaag aacacctgct gtgggaataa gctgcatggt atcgcggtga gtggccaggc
     8161 gcagccggaa ttgcaggaaa atctgtgtga aggaaatggg aaatgcggga tcgcctattt
     8221 tgaaagcgct ggcgggaagg cgtcgaagaa cacctgccgc gggaataagc tgcacggtgt
     8281 ctatgtaact gatcaagcgc acccggtgtt gcaggaaaac gtgtgtgagc gcaacgggga
     8341 ttgcgggatt gtctatttag gtagcgcgtt ggggacggcc cagaggaaca tttgtcgcgg
     8401 gaatggcgag gatgggatat acgctggtga acaatcgcag ccgatgttag aggaaaatct
     8461 gtgtgagggc aatggctgcg gtatcgccta tttcggtagc gctgggggga aggcgtggaa
     8521 aaacatctgc cgcgggaata aggtgcacgg tttcgaagtg actaacgagg cacagccggt
     8581 gttggaggaa aatctgtgtg agggaaacgg ggagtgtgga atcgcgtatc ttaatagcgc
     8641 gtcggggaag gcatggaaga acacctgtcg tgggaatgag aacagcggta tctggttggc
     8701 tgaccaggcg cagccggtgt tggaggagaa cctgtgtgaa gagaatgact gtgggatcgc
     8761 ctatttgggc agcgcttggg gcagggcatg gaagaatacg tgtcgcggga ataagcgtga
     8821 cggagtttcg gtgggtgggg agtcgctgcc cgtgttagaa gaaaacctgt gtgagaaaaa
     8881 tgcctttggg atcgcctatt gtggtagtgc cgcggggagg gcatccaaga atacctgccg
     8941 cgaaaacaag cggcacggag tgtacgtggg tggaagggca cagcccgtgt tagaagaaaa
     9001 cttgtgcgag ggaaacggtg attgcggcat cgcgtaccgt ggtagcgccg ccgggaaggc
     9061 gtggaagaac atctgccgcg gtaacaagaa ctacgacatc tacgttggaa
//
LOCUS       DAOSUT010000160         9107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4064_length_9107_cov_8.839925, whole genome shotgun sequence.
ACCESSION   DAOSUT010000160 DAOSUT010000000
VERSION     DAOSUT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_9107_cov_8.839925"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLD05_12810"
     CDS             complement(<1..574)
                     /locus_tag="PLD05_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOP78370.1"
                     /translation="MSRSQAFSLDKLLANAQLPALPQSAIHLLQVSQDPEAGPAEYAV
                     PIEADPGLASQVLRFVNSSYFGFSREISSVKQGITLVGTKTIKNFVLWSAVFSLMPCP
                     KCGPFDLRSLWQDSLRRALFAREMGKICRLEEREELFAAALLQDMAVPLLAKEVPDLY
                     VQLLTARNEGQRRLSTVERAVCGWDHAEAAV"
     gene            complement(970..1416)
                     /locus_tag="PLD05_12815"
     CDS             complement(970..1416)
                     /locus_tag="PLD05_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78371.1"
                     /translation="MLPDSENPFAPPQTTSTVSPLGDGLTWSLGIREIVVALVVLLVL
                     GGSVAAFYALVENSMIPAEERLLVEVEEDLDGMMVASLLTAIFVLGIVLWNEGILFSA
                     GGLIALLLGTVIWGVATAVLFTPLGALVIWVTVLLAVILGLVLLRR"
     gene            complement(1463..2368)
                     /locus_tag="PLD05_12820"
     CDS             complement(1463..2368)
                     /locus_tag="PLD05_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78372.1"
                     /translation="MSTIGQRMGITAARRAFTLVELLVVIAIIGILMAVMLPAIQASR
                     ETARRGQCGNNLRLIGQALTRYYGSYECFPAGVESEQGPIVNEPRGRHVGWICPLLPY
                     LDADVYYRKLDLSESVYAAKNEEVRKTALPAVRCPSFGRTSMSLEWAESNYAACYHDE
                     EAPIDTARNGAFVLNRWFTQDDITDGLAFTLFVSEKGAETGELGWASGTRATLRNTGT
                     PPANWLRLRAQLDQSTKDQDEKTQPETTPQSETVSKVGGFSSAHPGVVQALMGNGTVR
                     PVVWTIDQTVWKQMAHRCDGSLPGG"
     gene            complement(2401..3489)
                     /locus_tag="PLD05_12825"
     CDS             complement(2401..3489)
                     /locus_tag="PLD05_12825"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78373.1"
                     /translation="MRWYPGTESRELRDGKKLAVFPSSPCQRHGVTLTECLAVLLCIG
                     ILIGLLLPAVHSARETARRAQCQMNLQQIGLALRNYETLHTVLPAGTVENSGPVSNIP
                     LGFHHSWTVAILPWLDPPAGAHVRYDQSVYSPRNLQFLVSLGRTPSVYTCPSGLSFAS
                     ACADYAGCHDDRDKPIDEDDHGVFFRNSAVRSVEIPDGTAHMIFVGEKPRYGWEWGWM
                     SGTRATLRTTAIPIPQMLELIERQRIDGGPPMESAQDLIRMIRPDVWLTNVPFGDDVE
                     AAFSQASKVPELPEDPKVVEELQKFGEIPEGRPIIGGFGSEHPGVTNFLFGDMSVRPV
                     WHQVDPKVLRGLGNRLDGEGIPESLQNN"
     gene            complement(3520..4029)
                     /locus_tag="PLD05_12830"
     CDS             complement(3520..4029)
                     /locus_tag="PLD05_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78374.1"
                     /translation="MQRTVKLDYQPLDNMWQRGLIYDVARRLQTRGVAALAVIVCLII
                     VASLGINAIVDLKASWDDFRQARTALQADWLLASAFDRVRSRLAHDPAYEGEVWTIPA
                     EQLDGRSSAEIRTVLTRTPDSPGKVMAKVVLELRANGQTCITLTKVNPVEGVNRRTET
                     PRSSSEVPR"
     gene            complement(4066..4605)
                     /locus_tag="PLD05_12835"
     CDS             complement(4066..4605)
                     /locus_tag="PLD05_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOP78375.1"
                     /translation="MSNKRRGYTLIEMVAVAVLSTFILGAAVATTALVTGVSTSTSRE
                     IDFYHELARLANSFRSDAHRAREVSATGMEGVLKVPGDGAIIGEMKLADGRSVRYVVG
                     QSLMREVYRDGKLVARDGFELRRGQRVRVEKREKNGRVFVHLSVVGAPVFDKGDQLAA
                     GRVWEVQAVVGRDLLPVEP"
     gene            complement(4608..5120)
                     /locus_tag="PLD05_12840"
     CDS             complement(4608..5120)
                     /locus_tag="PLD05_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78376.1"
                     /translation="MVKTCSRRISQERHSRLERRFAVTTMEIIVAMFLLATLAMIASQ
                     IIATVVQTRVEQRKRAFALQEAGNAMEEAFLLSWQELKPGQSRQWTCSDTAAKILKNA
                     KVTLVTEATPDDNTRRLVVCVEWSSGGGREPQRIQLVAFRHRAARSEEAAAAREAKGT
                     EKGIAASEEK"
     gene            complement(5344..6585)
                     /locus_tag="PLD05_12845"
     CDS             complement(5344..6585)
                     /locus_tag="PLD05_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78377.1"
                     /translation="MFYEKVAAMAGLCVLGLVLLDLSRTRNVPLSRQLSWGRVWLRIF
                     GWACLFVGGFFVGNVFLAGYSLVIILVVAAAVSGRTPSWGGQEIWVTIAAGIRHQLPI
                     PSLVEAAVEESGQAARYRQLLARLRAGMPLEELVRRLRIGRPERTALALATKTGRFDE
                     AIEKAREYRSAMWDVEELFTGRMLLLLLLLQGVPAGAFMFARISGHFGLILKDFGLDA
                     QTAATRFGLGVPAWFFSPGLLAYIWTAVSFGIVVGILLYWLGGRGILADFWPWSRCSL
                     RSRSLILMTLGQGVRSGLSFTEIFDVLVNHPPTLGIRGRLLRTLEAMFRGDDPWDAMR
                     KSGILSPADKDLLAASQQLGELPLSLETLARLHRDRYSAALRWWAVIGYLVIVTLFVL
                     GAMIAYACCLGTLLAIIFCLM"
     gene            complement(6591..7649)
                     /locus_tag="PLD05_12850"
     CDS             complement(6591..7649)
                     /locus_tag="PLD05_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP78378.1"
                     /translation="MGNQPSDFEPGANWDVISEALSRTVEANLPLPTTLRALAADLDD
                     RRTASALKRIAARIERGESLDAIFSKEKSTKSRPVGQLLHAASLTPQPSETLNRFVTI
                     MEDYTSNLRNLRTALSYPFLCLTLALFGAFVISAGWLLPNARDIVAMEEELSLTQPVR
                     LRIELMWAGNLVSLLIGILVWGIGLLIILGPVRMSRCSLTYQLPMLGRYYAYLNLSLF
                     AKVMAHLTQSGVELSKALEAVAGLVSYPRLRRALKEAAAKAAESATFDEIVRTDQRFP
                     ATLRTFVTQSGSKLSLSARFLAAADFFEDRARALWHAAPVLLMVFAILGLIMLWMTYA
                     IPLMMWIYWIQLLSGLAG"
     gene            complement(7709..7954)
                     /locus_tag="PLD05_12855"
     CDS             complement(7709..7954)
                     /locus_tag="PLD05_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78379.1"
                     /translation="MAKARFCETCGDPIEEDWWMKSEYMMEAGQIARAARYCEDCGRE
                     IILGQLHAPRSEQQRWSRLASDGGWGKPPLENDTHDE"
     gene            complement(8026..>9107)
                     /locus_tag="PLD05_12860"
     CDS             complement(8026..>9107)
                     /locus_tag="PLD05_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP78380.1"
                     /translation="SHLPADERTAISADEFLALHRQVAALISLGLPIERHLAGWNRRQ
                     GRRLKRATRQVISSLQKGQTLSEALSQVDKDLAPVYRAVLEAGMASGRPGMALQELTE
                     CGQRILGIRSLLLSSLYYPLIVFLVAWAVVILYLLVPFPVLHEVIISWNGESAVFDWL
                     NQARATMHYWGPIGPVVILLLFLFWRFQLRKSKLLIPWRAAWRLGWFPGVRRLIQWQQ
                     NVVLSDMLALMTRQGVPLDRAVRLAGAIFADPKMERESGAMADAIAAQDWQRVLEVRR
                     AQRIPMSIRLAIVEGVRSGRLEDALMRVWQHWRRQTWRQDVWIRTIAVPLVVVAFGCV
                     CVLIVALTFWWPWLQVMFQMFSFRP"
BASE COUNT         2182 a         2850 c         2245 g         1830 t
ORIGIN      
        1 agaccgctgc ctcggcatga tcccagccgc aaacagcccg ctcaacggtg gacaaccgcc
       61 gttgcccctc gttccgcgcg gtaagaagtt gcacatacaa atcggggact tctttggcga
      121 ggaggggaac cgccatatcc tgcaagagag ccgccgcaaa gagctcctct ctttcttcca
      181 agcggcaaat ctttcccatt tctcgagcaa atagggcccg gcggagagaa tcctgccaaa
      241 gactacgcag atcaaagggg ccgcacttcg ggcaaggcat caaactgaaa acggcactcc
      301 agaggacaaa gttcttaatg gttttggtgc caacaagagt aatcccctgt ttgacactgg
      361 agatctctcg cgaaaaccca aaatatgagg aattgacaaa acgtaacact tgacttgcta
      421 acccgggatc ggcctcgatc ggcacggcat attcggctgg tccggcttct gggtcctggg
      481 aaacctgtag taaatggatc gcgctttgcg gcagcgcggg tagttgtgcg ttggctagta
      541 acttgtcgag cgaaaatgcc tgcgaccgcg acatatcgcc tccgatctat cgttgagtga
      601 aaacgaatac aatccccaac ttcccccaag ataattctgg acggggacat ggtcccgtac
      661 gctcaaagga ttcctataat agcctagaac accccagggg attgagcaaa tcttgactgg
      721 gaaaaagaga ctcttcggca ttccccgtaa gtggcaaatt gcccaactga ccgataaggc
      781 cgaaaaaggt gtcacccctt gccgtgccgt cgggcaggaa cacggcctgc cccgacttca
      841 tagaacacga aaggggcact ccctgtggcg gcccactgat caagattggc gcgtatcaca
      901 cctcttgatc ggtttcgctc ttaagccacc aggctcgcgg aaggcacgct ttaatgcatt
      961 ctgccgcaat caccttctga gtaacacaag tcccaggatc accgccagca gcaccgtgac
     1021 ccagatgacg agggcaccaa gcggcgtgaa caggaccgcg gtagctaccc cccaaatcac
     1081 tgtgccaagc aaaagcgcaa taaggccccc cgccgagaag aggatcccct cgttccaaag
     1141 cacgatgccc agcacaaaga tcgccgtcag caaagaggcc accatcattc cgtccaaatc
     1201 ttcctccact tccaccaaca acctctcttc agcggggatc atggagtttt caaccaaggc
     1261 ataaaatgct gccacacttc caccaaggac caagaggacc accaacgcaa ccacaatctc
     1321 acgaatgccc aggctccagg tcaatccgtc gccaagcggc gagaccgtgc ttgtcgtctg
     1381 cggcggggca aaagggttct cagaatcggg cagcatcggc tgagtccacc aaatctttca
     1441 agacgtgaca ttcatgggga aactaacccc ccggcagcga accatcgcac cgatgggcca
     1501 tctgcttcca aaccgtttga tcaatcgtcc aaaccacggg ccggacggtt ccattcccca
     1561 tcagggcctg taccacgccg ggatgagctg acgaaaaacc gccgaccttg ctcaccgtct
     1621 cactttgcgg tgttgtttcc ggttgtgtct tttcgtcttg atccttcgtt gattgatcga
     1681 gttgcgcacg aagccgcaac cagttcgccg gcggtgtgcc ggtattcctc aatgtggccc
     1741 ttgttcccga ggcccaaccg agttcgccag tctccgcacc cttctcactg acgaacaagg
     1801 taaatgccag gccgtcggtg atgtcatctt gggtaaacca gcggttcagg acaaaggcac
     1861 cgtttctggc cgtatcgatg ggtgcctctt cgtcgtgata gcatgcagca tagttcgact
     1921 cggcccactc caaagacatc gaggtcctac cgaaagaggg acaccgcacg gccggtaacg
     1981 ccgttttccg cacctcttcg ttcttggcag catacacact ctcggacaaa tccaatttgc
     2041 ggtaatagac gtcggcatcc aagtacggca acaagggaca gatccagccg acgtgtcgtc
     2101 cccgcggttc attgacgatg gggccttgtt cgctttccac gccggctgga aagcactcat
     2161 agctgccgta atatcgcgtg agcgcttggc cgatgagcct caaattgttc ccacactgtc
     2221 cccggcgtgc cgtttcgcgc gaggcctgaa tggctggtaa catgaccgcc atcaagatgc
     2281 cgatgatcgc aatgaccacc aagagttcca ccagcgtgaa ggctcgccgc gcagccgtta
     2341 ttcccattcg ttggccgatc gttgacatag gatttgtcct ttgccttaag aactcatggt
     2401 ttagttgttt tgcaaggatt caggtatccc ttcgccatcc aaacgattgc cgaggccacg
     2461 caggaccttc ggatcgacct ggtgccacac gggacgcacc gacatatcgc caaacagaaa
     2521 attggtcaca ccaggatgtt cagagccaaa tccgccaatg atgggccgac cttcaggaat
     2581 ttccccaaat ttttgaagtt cttcaaccac ctttgggtcc tcaggcaatt ccggcacctt
     2641 cgacgcttgg gaaaacgccg cttcaacatc atcgccaaat ggcacatttg ttaaccacac
     2701 atcgggtcga atcatccgta tcaaatcttg agcactctcc atcggcgggc ccccgtcaat
     2761 gcgttgacgc tcgatgagtt cgagcatctg ggggatgggg atggccgtcg tccgcaaggt
     2821 cgcgcgggtt ccactcatcc atccccattc ccaaccatac cggggctttt ctcccacgaa
     2881 aatcatgtgg gccgttccat cgggaatctc aacactccgc accgcactgt tgcgaaaaaa
     2941 gacaccatgg tcgtcttcgt caatcggctt atcccgatcg tcatgacatc ccgcataatc
     3001 agcgcaagca gacgcaaagg acaaccccga agggcaagtg tacacagatg gggtacgtcc
     3061 tagcgaaaca agaaactgca agttacgcgg actgtaaacg ctttgatcat atcgcacatg
     3121 ggcgcctgcc ggagggtcca accacggtaa gatggcgact gtccaactat gatgaaaacc
     3181 caggggtatg ttcgaaacgg ggccgctgtt ctctactgtc ccggcaggga gcaccgtatg
     3241 aagtgtctcg tagttccgca aggccaggcc aatttgctgg agattcatct ggcattgggc
     3301 acgccgggcc gtttcgcggg cactatgaac ggccggaagc aacaggccga tcaaaatacc
     3361 gatgcagaga aggacggcaa ggcactccgt caaggtcacg ccatgccgct ggcatgggct
     3421 cgacggaaaa accgccagct ttttgccgtc ccgcaattca cgcgactcag tcccaggata
     3481 ccacctcacg gggtaactcc tcatgatgcg caaaccgatt caacgcggga cctccgaact
     3541 gctcctcggc gtttcggttc tccggttcac cccttccacc gggttgacct tcgtcaaggt
     3601 gatgcaggtt tgaccattgg ccctaagctc aagcacaact ttcgccataa ctttgccggg
     3661 cgagtcaggc gtccgagtga gtactgtcct gatctcggcc gacgagcgtc catccagttg
     3721 ctccgccggg attgtccaaa cttccccttc gtaggccgga tcgtgggcca agcgacttct
     3781 cacccgatca aacgccgagg caagcagcca gtcggcctga agtgctgtcc gcgcttgtcg
     3841 aaaatcatcc caactcgcct tgaggtccac gatcgcattt atccccagcg aagccacaat
     3901 gatcagacat acaatcacgg ccagggctgc cacgccacgt gtttgcaaac gccgggcaac
     3961 gtcgtaaatc aacccccgct gccacatgtt atctaacggc tggtaatcta gctttactgt
     4021 ccgctgcatg gctgacctgc ttttatcttc gtcccatatc acgtgctatg gctcaactgg
     4081 caagagatcg cggccaacga ctgcctgcac ctcccaaaca cgtcctgctg ccaattggtc
     4141 acccttgtcg aacacaggtg cccccacgac actaagatgg acaaacaccc ggccgttctt
     4201 ctcgcgtttt tccacgcgaa ctcgttgtcc ccgccggagt tcaaagccat cccgggcaac
     4261 caatttgccg tctcgataaa cctctcgcat taggctttgg cccaccacat aacgcacgct
     4321 tcgaccatcc gccagcttca tttcgccaat aatcgcgccg tcaccgggga ctttgaggac
     4381 gccctccata cctgtggcgc tgacctccct tgcgcgatgg gcatcacttc gaaaactatt
     4441 ggccagtcgc gccagctcgt gataaaaatc aatttcccga gatgtgctgg tactcactcc
     4501 cgtcacgagg gcggtcgtcg ccacggctgc acccaaaata aatgtcgaga gcacagccac
     4561 cgctaccatc tcgatgagag tatatccccg ccgtttgttg ctcacgatca tttctcctca
     4621 cttgccgcaa tgcccttttc ggtccccttt gcctcacgcg ccgccgcggc ctcctctgat
     4681 ctcgcagcgc gatgccgaaa cgccacaagt tgaatccgtt gcggctccct cccaccgccg
     4741 gatgaccatt cgacacacac gacaagccgc cgggtattgt catccggcgt ggcctccgtg
     4801 accagcgtca ctttggcgtt tttcaaaatc ttggcggccg tgtccgagca cgtccactgc
     4861 cggctttgcc ccggtttcag ttcttgccat gataagagga aggcctcttc catggcattt
     4921 cctgcctcct gcaaggcaaa ggcccgtttt cgctgctcga cccgagtttg aacgaccgtg
     4981 gcgataattt ggctggcaat cattgccaag gtcgccagga gaaacatcgc cacgatgatc
     5041 tccatggtcg tcacggcaaa gcgacgttca agcctactgt gacgttcttg gctgatcctt
     5101 cgactgcatg tcttgaccat tggttgcttc acattcaatc aaatactaag agccaatcta
     5161 atctcccctc acccgttcgg cgggagaggg gtcgggggta agggtggctc gaatcactgc
     5221 cggttttcgt aatcgattct cacgttcgct tgccctcacc ccaaccctct cccggaggga
     5281 gaggggggac tctttgggat agactctcag caacatttcc gttccgcaag gctgtcaagc
     5341 tactcacatt aggcagaaaa taattgccaa aagcgtccca aggcaacatg cgtacgcaat
     5401 cattgctcct aagacgaata acgtgacgat caccaagtag ccgatgacgg cccaccaacg
     5461 aagggccgcg ctgtagcgat cgcggtgcaa cctggccaac gtttccaagc tcagtggcaa
     5521 ctctccaagc tgttgtgacg cggccagaag gtccttgtcc gccggcgaaa ggataccgct
     5581 tttccgcatg gcatcccagg ggtcatcacc gcgaaacatg gcctccagtg tcctcaacag
     5641 tcgtccgcga attcccaatg tgggaggatg attgacgagg acatcgaaga tttccgtaaa
     5701 agacaaccct gaccgaaccc cctgccctaa tgtcatgagg atcagacttc gggaccggag
     5761 tgaacaccgc gaccatggcc aaaagtcagc caggataccg cgaccaccaa gccagtaaag
     5821 caaaatccca accacaatac caaagctgac cgccgtccaa atgtatgcca acaaccccgg
     5881 cgagaaaaac cacgcgggaa cccccaagcc aaaacgggtt gccgctgtct gagcatccaa
     5941 cccaaaatct ttcagaatga gtccgaaatg tcccgatatt cttgcgaaca tgaatgcacc
     6001 cgccggaacc ccctgcaaca gcaatagcag aaggagcatc cgccctgtaa acagctcttc
     6061 cacatcccac attgctgagc gatattctcg agccttctca atggcctcat caaatcgtcc
     6121 cgtcttggtg gcgagagcca aggcagttcg ttcaggacgc cctatccgca acctccgaac
     6181 cagctcttcc aggggcattc cggcgcgaag tctcgccagt aattggcggt accgtgcggc
     6241 ttggccagat tcttccacgg ccgcttcgac aagtgatgga atcggcaatt gatgacgaat
     6301 cccggctgca atcgtcaccc agatttcctg tcctccccaa gacggcgtcc gtcccgaaac
     6361 cgctgccgca accaccagga tgataaccag tgaatatcct gctaaaaaaa cgttgccgac
     6421 gaaaaatccc cccacaaata aacatgccca tccgaatatg cggagccata cccttcccca
     6481 gctcaattgt ctcgagagcg gtacattgcg cgtgcgggac aggtctagca ataccaatcc
     6541 caacacacac aatcccgcca tcgccgccac tttctcataa aacataaccg tcatcccgct
     6601 aggccagaaa gcagctggat ccagtaaatc cacatcatga gaggaatggc gtaggtcatc
     6661 caaagcataa ttaatcccag gatggcaaac accatcaata aaaccggtgc ggcatgccag
     6721 agcgcgcgtg cccggtcctc aaaaaagtcc gccgctgcca gaaagcgggc cgacagcgaa
     6781 agcttacttc ctgattgcgt tacgaacgtc cgtaacgttg ccggaaaacg ttgatcggtg
     6841 cgcacgatct cgtcgaacgt tgcgctttcg gcagccttcg ccgctgcctc tttcaaagca
     6901 cgccgcaggc gggggtatga aacgagtcct gcaacggctt ccagggcctt tgacaattcc
     6961 acgcccgact gcgtcaaatg ggccatcact ttggcaaaaa ggcttaagtt cagataggcg
     7021 tagtacctcc ccaacatggg taactgataa gtcagcgagc aacgtgacat ccgtaccggc
     7081 ccgaggatga tcagaaggcc gatgccccat acaagaatgc cgattaagag agaaaccaaa
     7141 ttacctgccc acatcaattc aatcctcagc ctgacaggct gggttaggga aagctcctct
     7201 tccatagcga cgatatctct tgcgttggga agcaaccacc ctgcactgat aacaaatgcg
     7261 ccaaagagcg ccagcgtgag acagagaaac ggataactga gggccgtccg aagattccgg
     7321 aggttggacg tgtaatcctc catgatggtc acgaatcggt ttagcgtttc ggaaggttgg
     7381 ggggtcaaag aggctgcatg aaggagttgc cccaccggcc tggatttcgt cgatttctcc
     7441 ttggaaaaaa tggcgtccag tgattcgccc cgctcgattc gggcagcaat cctcttgagc
     7501 gccgacgccg tccttctgtc gtccaggtcg gcggccaagg cccgcagtgt cgtgggaagc
     7561 ggcaagttcg cctcaaccgt tcgactgagg gcctcagaga tcacgtccca gttggcaccc
     7621 ggttcgaaat ccgatggttg attacccatt atcaccgcca cgtgtcttct aaggacgtta
     7681 cgccggcgtc agccaaacat acaggacatc attcatcgtg ggtatcattt tccaaaggcg
     7741 gcttccccca accgccgtcc gatgccaggc gagaccaccg ctgctgctcg gaacgaggcg
     7801 catgcagttg tccgaggata atctcccggc cacagtcctc gcaatatctc gcggcccgcg
     7861 ctatttgccc cgcttccatc atgtactcgc ttttcatcca ccaatcttcc tcgatgggat
     7921 cgccacatgt ttcgcagaaa cgagctttcg ccatggcctt ccctcccaac taaagacctt
     7981 tcttgcctga agagacaacc tggccgtttc ccgaagctca ccgcgtcacg gacggaagct
     8041 gaacatttga aacatcactt gaagccacgg ccaccaaaac gtcaaagcaa caatcagcac
     8101 gcacacgcaa ccgaaggcca cgaccaccag cggcacagcg attgtcctaa tccaaacgtc
     8161 ctgacgccag gtttgccgcc tccaatgctg ccagacacgc atcagggcgt cttcaagtct
     8221 tcctgaacgc accccctcaa caatggccaa gcggatggac atggggatcc gctgcgcacg
     8281 tctgacttca agaacccgct gccaatcttg agcggcaatg gcgtccgcca tcgcgcctga
     8341 ttcccgctcc atttttgggt ccgcaaaaat cgcccccgcc aaacgcacgg cacgatccag
     8401 cggcaccccc tgccgagtca ttaaggccag catatcggag aggacaacgt tttgctgcca
     8461 ttggataagc cgtcggacgc ccggaaacca tcctagccgc caggctgccc gccacggaat
     8521 tagcagcttc gattttctca attgaaatcg ccagaacaaa aagagcaaca agatcactac
     8581 cgggccgatc ggtccccagt aatgcatcgt cgcgcgggct tgattgagcc agtcaaaaac
     8641 cgcgctttcg ccgttccagg agatgatgac ctcatggaga accggaaacg gcacaagcaa
     8701 gtagaggatg acaacggccc aggccacgag aaacacaatc agcggataat aaagggacga
     8761 aagcagcaaa ctacgaatcc ccaaaatccg ttgcccacac tctgttaatt cctgaagggc
     8821 cattcccggt cgtccgctcg ccataccggc ttccagcacc gcccgataaa ccggtgcgag
     8881 gtccttatcc acctgagaga gcgcttcact aagcgtctgg cctttttgca ggctacttat
     8941 tacttgacga gttgctcgct tcaagcgacg cccctggcga cgattccagc ctgccaagtg
     9001 gcgttcgata ggaagaccca gcgagatgag agccgcaacc tggcgatgca acgctaagaa
     9061 ctcatccgcc gaaatcgccg tccgctcatc agcagggaga tgagaca
//
LOCUS       DAOSUT010000161         9065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4087_length_9065_cov_9.788790, whole genome shotgun sequence.
ACCESSION   DAOSUT010000161 DAOSUT010000000
VERSION     DAOSUT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_9065_cov_9.788790"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1287
                     /gene="rsmA"
                     /locus_tag="PLD05_12865"
     CDS             283..1287
                     /gene="rsmA"
                     /locus_tag="PLD05_12865"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOP78381.1"
                     /translation="MTPGGSPRQTLTFLRERFAAAGIRPKTDLGQNFLIDYNLLRVLV
                     ETAEVSLQDVVLEVGTGTGSLTNMLAERAAYVVSVEIDPRLHALARQLVGEKPNVCLL
                     CQDALKNKNAIHPGIIERIRQALDVDPQRRLKLVANLPYCVATPVISNLLSLREPPVS
                     MTATIQRELAERIIAQPGSKDYGALSVWVQSQCRVHLERTIPPQAFWPRPKVFSAIIH
                     IESDAQRRSRIEDLAFFHGWIRRLFLHRRKFLRSQVLSALGRDASKPDVDAMLQELGL
                     DSQLRAEQLSISQIIELAEAARKRFPTLVDSIRGSSSEADEDAASSEGVKEDQADSED
                     "
     gene            1368..1622
                     /locus_tag="PLD05_12870"
     CDS             1368..1622
                     /locus_tag="PLD05_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78382.1"
                     /translation="MYEDTSYPKTQFIEMLGRQLLRVVTEGGPTPAELAKVGLKEDSI
                     VRVNRQGDIELRRPDRWDVIGGLIGDFEARIKQETGLDWV"
     gene            complement(1591..2631)
                     /locus_tag="PLD05_12875"
     CDS             complement(1591..2631)
                     /locus_tag="PLD05_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOP78383.1"
                     /translation="MKHPQKALCRRAASFLSNSSAASTAEYALLLTLVIIVGAAGFFG
                     LSTFTRWVFQHNAQIASSSEPGSDRTSSSSTKPSETSFYLPHRIIERAIRTLDKAELA
                     IQFAAGLLGLAMGSGLVIVCWQVIHSRRRPRPEEEPTTIYRASQTSEHETLYAKRAVL
                     RRVLRRAIDMRQAAELSVAKLMSENVVAVHPKAPIYKVVQTIKEKCVSHVLVVDRKGI
                     LLGIISHRDLSRRTGRYAEDIMTRSPVTVHVSSSATHAISLMLEKDISCLPVVEDGHV
                     RGVLTKTDVMIGFQALIQALELVLTEPAEEEELDSFTENGVAPDESPGDTPAAIPTEI
                     SRITPNPNPSLA"
     gene            complement(2662..4110)
                     /locus_tag="PLD05_12880"
     CDS             complement(2662..4110)
                     /locus_tag="PLD05_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP78384.1"
                     /translation="MDRTFQSATESQPTSGTDRLDEIVERLNRLVGSSKPRTETLPPA
                     PSTVKPATLSASSLEEWAETDFIPVEPTTLEEAQLTESEVEALILKYLLARGDATGRA
                     IADQVKLPFIIVDAIMRRMKADRLVAHRGDAPMNDYVYELSDLGRERARRYSQICSYF
                     GAAPVSLSDYVTSVAIQSLTKQRPTRQDLERAFEGLVLSEEMLNRLGPAVNSGRGLFL
                     FGAPGNGKTSIAERVTAAFGPYIWIPRAIGVDGEIIRLFDPVNHFEAPLPEPEGLLDT
                     RHIDKRWVRIRRPTIIAGGELTMSALEVTVNRASGICEAPLQLKSNCGTLVIDDFGRQ
                     RMPIQELLNRWIVPLERRYDFLNLPNGKKITVPFDQLIIFSTNLEPRQLVDEAFLRRI
                     PYKIEVVDPTEEQFRQLFKILAPQLGIRYCEEAVDYLIERYYKAVGRPFRFCQPRDLM
                     LQILHYCNYQEIEPEMKKEYFDIAVANYFSVM"
     gene            4500..5351
                     /locus_tag="PLD05_12885"
     CDS             4500..5351
                     /locus_tag="PLD05_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOP78385.1"
                     /translation="MVKNTLSKVSDSESRAGKCALKVGGVCYLNARPLLQHLADLCPH
                     IVIEYDVPSRLAAKLAAGQLDIAIIPAVEALRGDGYVVVSDACISAKEAAKSVILFSR
                     VPLDQIRSVALDAGSRMSAALTRVLLAEWYGVTPEIRDFPLEKRPEEAKTDAVLLIGD
                     RAMVTKQPPGYPLRFDLGSEWRKWTDRPFVFALWVARSGVTDLDSWHKVFSLARDRGL
                     DDLVNIAQAASRDLGLSFDECHQYLSKNMHYRLGEDELESLTLFRSLAVKHGILAPSA
                     RLTFHGG"
     gene            5341..6402
                     /gene="mqnC"
                     /locus_tag="PLD05_12890"
     CDS             5341..6402
                     /gene="mqnC"
                     /locus_tag="PLD05_12890"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOP78386.1"
                     /translation="MVVSILDRATSGQRLGDEEALFLFEHASLALLGEAADAVTRRLH
                     PENYRTYNIDRNINYTNICVSGCKFCAFYRPPGHPDGYVIDYPTIFQKIEETIKLGGN
                     QILLQGGLNPSLPLTWYEELLQAIKQRFPQINIHGFSPPEIHFLAKREKITVEEVLRR
                     LKNAGLGSLPGGGAEILVDRVRKELKSHKVDTEGWLEVCRTWHRLGGRGSATMMFGHV
                     ETFADRVAHLRRLRDLQDETGGFTAFICWTFQPKNTALSHLRKHGAYDYLRTLAISRL
                     YLDNFANIQASWVTQGAAVGQLSLRFGANDMGSLMIEENVVAAAGTRFRTSESELRQL
                     ITQAGYVPRRRNVFYELVD"
     gene            6506..7558
                     /locus_tag="PLD05_12895"
     CDS             6506..7558
                     /locus_tag="PLD05_12895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOP78387.1"
                     /translation="MAMSDSAAGELPFPTDFVARLRDLIPPEHLQKCLESFWGQRWVS
                     FRVNTLKVTAQELEKELEALGISFQRVPWLPEAYLAPPENRETLVNTHAFLEGRIYIQ
                     DLSSMLAVHVLDPQPGEEILDLAAAPGGKTCHVAARMKNQGRIAAVEVIPQRLYKLVA
                     NLERSGVTIVKTYLADGRTIGKKTPNRFDRVLLDAPCSSEARFDARDPASWKHWNLRK
                     IAECARKQKGLLLSALEAVRPGGLVLYCTCSFAPEENEAVVDHVLQQAQGGVFVEPLK
                     IPLPNAIPGLTKWQGREYHPSLELACRILPTHEMHGFFMCRLRKGEEATETYRRRERK
                     WSRSPKHRARGKDALS"
     gene            7809..9065
                     /locus_tag="PLD05_12900"
     CDS             7809..9065
                     /locus_tag="PLD05_12900"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOP78388.1"
                     /translation="MSASDRQTTPADFPLEQPRLAWFFGVLLAVVVWSYWNSLSEAAI
                     AWRGAQYSHGLFVPLIATFLLWVRRLGGTFDWQWQTTTEKWRLVAVGGVLALAWFSYI
                     ASERLGQQAAPGEAVSLTAASGLGFMAAMLYATGLLTWATFAADSWLQHSLESEKQRQ
                     NDEDKTHLRGELAGEALASGTQTALVRGRYVGLFILIVAVVLRLASAYVGLDVAEMWT
                     LPLGLLGAGVCTIGWRSLRWSWPAVVVLFFAFPLPFGVERTILVPLQGIAAQGGAYLL
                     QSLGLEASTEGAHFIRLGEFQLGVVEQCSGLRMTTVFITLVFVYVVLVPTPKWQIPVL
                     LLSSIPIALVVNILRITVTGTLYVYGSRELAQHVFHDWAGYLMPLAAVALLLLLRVLL
                     DNLFIKVEEQDVPLAQRLEPAKKGST"
BASE COUNT         1945 a         2375 c         2664 g         2081 t
ORIGIN      
        1 tgggaaaggg ttggggtgag ggcaagcgat cgtcggaatc ggttgagaaa accggcagcg
       61 attcgaggca ccgtcagccc cgacccctct cccgacgacc atgtcagggg aattatggga
      121 taggctctaa cgaaaaaggc gggcgtggtg ggtttttcca gaatgggtcg gagccacgaa
      181 tgaggtcttc aagcgattcc ctgccgttta ctggcaagtt gcaaagcgct aatcgctggc
      241 gagtgcaaaa gccgtatggg tggtgaagga cgcagtagcg gaatgacacc aggtggttct
      301 ccccgacaga cgctgacatt tctcagggag cggtttgcag cggctggcat tcggccgaag
      361 accgacctcg gtcaaaattt cttgatcgac tataatctgc tgcgggttct agttgagacg
      421 gccgaagtat ccttgcagga tgtcgtgctg gaagtcggga cggggaccgg cagtctgacc
      481 aacatgctgg ccgaacgagc ggcttacgtc gtgtcggtcg aaatcgatcc caggctgcac
      541 gccttagccc gccagttggt cggagagaag ccgaatgtct gtcttttgtg ccaggacgct
      601 ttgaagaaca aaaacgcgat tcatcccggg atcattgagc ggatccggca ggccctcgat
      661 gtggatcctc agcgccgatt gaagttggtg gccaatctgc cctattgtgt ggcgacgccg
      721 gtgatcagca atttgctgag cttgcgtgag ccccctgtct ccatgacggc gacgatccag
      781 cgggagctgg ccgagcgaat catcgcccag ccgggctcaa aagactatgg ggcgttgagt
      841 gtgtgggttc aaagtcaatg tcgcgtacat ttggaacgga ccatcccacc ccaggcgttt
      901 tggccccggc cgaaggtatt ttcggccatc atccacatcg agagtgacgc ccaacgtcgc
      961 agccgcattg aagacctagc cttttttcac gggtggattc gcaggttgtt tctccatcgc
     1021 cggaagttcc tgcgaagcca agtcttgagt gctctcggtc gagacgcaag taagccagat
     1081 gtggacgcga tgctacagga acttggcctt gattcgcaat tgcgggctga gcagttgagc
     1141 atttcccaaa tcattgagtt ggcggaagcg gctcggaaac gatttcccac attggttgat
     1201 tcgatccggg gaagtagttc ggaggccgat gaagacgcgg cgtcgtcaga aggcgtgaaa
     1261 gaagaccagg cagatagcga agattgaccg gcgagggcag ggtaggttat cttgtttcaa
     1321 aatggaggtg tcgtgtcgat caggtcatcc tgtcaagtag gcaaactatg tacgaggata
     1381 cttcttaccc taagacccaa ttcattgaga tgttgggccg acagcttctc cgtgtcgtga
     1441 cggaaggcgg tcccacaccg gcagagctgg caaaggttgg gctcaaagag gatagcatcg
     1501 ttcgggtaaa tcggcagggc gatatcgaat tgcgtcgtcc cgatcgatgg gacgtgattg
     1561 gtgggctgat cggggacttt gaagcccgca tcaagcaaga gacgggtttg gattgggtgt
     1621 gatcctcgag atttctgtcg ggatggctgc gggggtatcg ccgggcgatt cgtccggggc
     1681 aacgccgttt tctgtgaaac tgtccaactc ctcctcttcg gcaggttctg tgagaacaag
     1741 ctccaaggcc tgaataaggg cttgaaatcc gatcataaca tcggttttgg tgaggacgcc
     1801 ccggacgtgg ccatcttcca caacaggcaa acaggaaatg tccttttcca acatcagaga
     1861 aatagcgtga gtcgccgagc tcgaaacgtg cacggttacc ggtgatcgcg tcatgatgtc
     1921 ttctgcgtag cgaccggttc gtcgggacag gtcacgatgg ctgatgatgc ccaggagtat
     1981 tccctttcga tcaacaacaa ggacatggct tacgcatttt tccttgattg tttggacgac
     2041 cttgtaaata ggggcctttg ggtggactgc gacaacattc tctgacatca atttggccac
     2101 ggatagctcg gcggcttgac gcatgtcgat agcacgcctt aatacacgcc gaagcacagc
     2161 acgtttggcg tagagagtct cgtgttccga cgtctgcgac gcccgataga tggttgtggg
     2221 ctcttcctcg gggcgaggcc gtcgtctgct gtggataact tgccagcaaa cgatgacaag
     2281 gccagacccc atcgccagtc ccagcagacc cgccgcaaac tgaatagcca gttcggcctt
     2341 gtcaagagta cggatggccc tttcgatgat gcggtgtggg agatagaaac ttgtttcaga
     2401 cggcttggtc gaggagcttg acgtgcgatc gcttccaggc tccgaagagg aggcaatctg
     2461 ggcattgtgt tggaataccc aacgggtaaa agtagataaa ccaaagaacc cggctgctcc
     2521 tacgataatg accagggtca agagcaaagc atattcagcc gtgctcgcgg ctgatgagtt
     2581 gcttaggaag cttgcagccc ttcgacagag cgccttttgc ggatgcttca tttcggctgg
     2641 ctcccgacct acccacgggc ctcacatgac ggagaagtaa ttggccacgg caatgtcaaa
     2701 gtactctttt ttcatctccg gttcgatttc ttggtaattg cagtagtgca gaatctggag
     2761 catgaggtcc ctcggttgac agaagcgaaa cggacgacca acggccttgt agtagcgttc
     2821 gatcaggtaa tccacggcct cttcacagta gcgaatgcca agttgtggcg cgagaatctt
     2881 gaaaagttgg cggaattgct cctcagtggg atcaacaacc tcaatcttgt acggaatacg
     2941 gcggaggaac gcctcatcaa caagttgccg aggttccaag ttggtggaaa aaatgatcaa
     3001 ttggtcaaat gggacagtaa ttttctttcc attgggcaag ttgagaaagt cgtaacgtcg
     3061 ttccaagggg acgatccacc gattgaggag ttcttggatc ggcattcgtt gtcggccaaa
     3121 gtcgtcgatg accagcgtcc cacagttact cttgagctgc aggggggctt cacaaatgcc
     3181 ggaggcgcga ttgaccgtga cctccaaggc cgacatggtg agctcaccac cggcgatgat
     3241 ggtcggacgg cgaatacgta cccagcgttt gtcgatatgc cgcgtgtcga ggagtccctc
     3301 aggttccggc aagggggctt caaagtgatt gaccgggtcg aagagccgaa taatctcgcc
     3361 gtcaactcca atggcccttg gaatccagat ataagggccg aaggccgccg ttacgcgttc
     3421 cgcgatgctc gttttcccat taccgggtgc gccgaacaaa aacaaacccc gcccggagtt
     3481 gacggcgggg cccagccggt tgagcatctc ttcgctaaga acgaggcctt cgaacgcacg
     3541 ctctaggtcc tgccgtgtgg gccgctgctt ggtcaacgat tgaatggcca cgctggtgac
     3601 gtaatcgcta agagagaccg gtgccgcacc gaagtaggag cagatttggc tataacggcg
     3661 tgcccgttcc cgtccgaggt cactcagctc ataaacataa tcgttcatcg gtgcgtcgcc
     3721 gcgatgggcg acaagtctat cggccttcat gcgacgcatg atcgcgtcca caatgatgaa
     3781 cggcagtttc acctggtccg caatggctcg accggtagcg tctcctctgg ctaataagta
     3841 tttgaggatg agcgcttcaa cctcgctctc cgtaagttga gcttcttcaa gggtggttgg
     3901 ctccacggga ataaagtctg tctcagccca ttcctccagc gatgaggccg aaagtgtggc
     3961 cggtttgacg gtgctcggtg caggcggaag ggtttcagtt cgaggtttgg agctaccgac
     4021 caaacggttt agccgctcga cgatttcatc caagcggtca gtgccggacg tgggctggct
     4081 ctctgtggca gattggaatg tccggtccat ttcgtgctcc tatttggagt ttttatcacg
     4141 acttgtgaga caccgatctt tctcattccg gcacaatcct tttgacccac aacgtgggcg
     4201 gaatcgggct gctccctggc caagacccat ttacatgagt gtcccgtatt aaatactagg
     4261 ttctccgtgg ccctatgagg gctccacggt gattggtgaa ctgcgaagat aagcccacac
     4321 ctttgcgact gcggtcgtca caatccttaa aatgcatata agccgcaaaa cacggtgatg
     4381 cctggaagtg ggtctgcggg cttagtggcg tgttcatcgg tgagaacggt ctgtattgac
     4441 catagttggc atacgtcgtt tacgctcaag gatggagcgg gaaaccgtag gaggttgatt
     4501 tggtcaaaaa taccttaagc aaagtctccg attccgaatc acgcgctggg aaatgtgccc
     4561 tcaaagtcgg tggggtgtgc tacttgaatg cgcggccact tctccagcac ttggccgatc
     4621 tgtgccccca cattgtgatc gaatatgacg tccccagtcg gctcgcggcc aaactggcgg
     4681 cgggacaact tgacatcgcc attattccgg ccgtggaggc cctgcgaggt gatgggtacg
     4741 tcgtcgtctc agacgcttgc atttcagcaa aagaggctgc taaaagcgtc attttgttct
     4801 cgcgggtccc gttagatcag attcggtcgg tcgccctaga cgccggttcc cgtatgagcg
     4861 ccgccttgac gcgggttctg ctcgctgaat ggtatggcgt gacgccggag atacgtgatt
     4921 tcccgttgga aaagcggccc gaagaagcga aaaccgacgc cgtcctgtta atcggcgatc
     4981 gtgctatggt gacgaaacag cccccgggct atcccttgcg gttcgacctg gggtccgaat
     5041 ggcggaaatg gaccgatcgg ccgtttgtat tcgccctgtg ggtggcacgg tccggcgtca
     5101 ccgatcttga ctcatggcat aaagtcttta gccttgcccg ggatcgtggc ctggatgatt
     5161 tggtcaacat tgcccaagcg gcatcgcgtg acctgggctt gtcctttgac gagtgtcacc
     5221 aatacttatc aaagaacatg cattacaggc ttggggaaga cgagcttgaa tctttgactc
     5281 tctttcgatc gttagccgta aaacatggaa tccttgcacc ttcagccaga ctcacattcc
     5341 atggtggtta gtatcctaga cagagcgacc tcggggcaaa gactcggcga cgaagaggcc
     5401 ctttttctgt tcgaacatgc gagcctagcc ctcttggggg aagcggcaga cgccgtaaca
     5461 cgccggttgc atcctgaaaa ctatcggacg tacaacatcg atcggaacat taattacacc
     5521 aatatctgcg tttccggatg caagttctgt gcattctacc gtcccccggg ccatcccgat
     5581 ggctacgtaa ttgactatcc gacaatcttc caaaaaatcg aggagacgat caaactgggc
     5641 ggaaaccaga tccttttaca ggggggactt aatccttctt tgccgttaac ctggtacgaa
     5701 gagctgcttc aagctataaa acagcgcttt ccgcaaatta atatccatgg ctttagccct
     5761 cccgagattc atttcctggc caaacgcgaa aaaatcactg tggaggaggt cttgcgtcgg
     5821 ctaaaaaatg cgggattggg aagcctgccc ggcggaggtg ccgaaatcct cgtggaccgt
     5881 gtccgcaagg agctaaaaag ccataaggtc gatacggaag gctggctgga agtctgtcgc
     5941 acctggcatc gcctgggggg gcgggggtcc gccacgatga tgttcggaca tgtggagaca
     6001 ttcgccgatc gggtggccca tctccgacgc ctccgggatc ttcaggatga gacaggtggg
     6061 ttcaccgctt ttatctgttg gacgtttcag cccaaaaata ccgccctcag ccatctacga
     6121 aaacacgggg cctatgatta cctccggacc ttggccatca gtcggcttta cctcgataac
     6181 tttgccaaca tccaggcaag ctgggtaacg cagggggccg ccgtgggaca gttgtcgctc
     6241 cgcttcgggg ccaatgatat gggtagcctg atgatcgagg aaaacgtggt agctgcagca
     6301 gggacccgtt ttcgcacctc cgagtccgag cttcgccagt tgatcaccca ggccggttat
     6361 gtgccccgcc gccgaaacgt tttttatgag cttgtcgatt agcattcgcg agcaagggtc
     6421 accgaagaat gccagtctcg tatgataacg ttatgggcgt ctctttggaa acagacgccc
     6481 ttggctttgc acgagacgga taagtatggc catgtcagac tctgccgccg gcgaattacc
     6541 ctttcccacc gactttgtgg cacgattgcg cgatcttatc ccgccggagc accttcaaaa
     6601 atgtttggag agtttctggg ggcaacgctg ggtgtccttc cgcgtgaaca cactgaaggt
     6661 caccgcgcag gagcttgaga aggaacttga ggcgttgggc atcagctttc agcgcgtccc
     6721 ctggctgccg gaagcttatc tggccccgcc ggaaaaccga gagactctgg ttaacactca
     6781 tgcgtttctc gaggggcgga tctatattca ggatttgtcg agcatgctgg cggtccacgt
     6841 tttggacccg caacccggcg aagagatcct cgacctggcg gcggcccccg gcgggaaaac
     6901 ctgtcatgta gccgcccgga tgaagaatca aggccgtatt gcggccgtgg aagtcatacc
     6961 tcaacgcctc tacaaacttg tggcgaacct ggaacggtcc ggcgtcacga ttgtcaaaac
     7021 ctatttggcg gatggccgca cgatcggcaa gaagacccct aaccgcttcg acagagtctt
     7081 acttgacgcg ccttgctcgt ccgaggcccg ctttgatgcc cgcgatccgg ccagttggaa
     7141 acactggaac ctgcgcaaga tcgccgaatg tgctcggaag caaaagggat tgctgctttc
     7201 ggcgctggag gccgttcggc cgggcggact tgtcctctat tgcacgtgtt cctttgcgcc
     7261 ggaggagaac gaggctgttg tggatcacgt tcttcaacag gcgcagggcg gtgtctttgt
     7321 cgaaccgtta aagattccat taccgaatgc aatccccggt ttaacaaagt ggcagggacg
     7381 tgaataccat ccctcgctgg aattggcttg tcgaatcctt cccacgcacg agatgcatgg
     7441 cttctttatg tgtcgtctta ggaaagggga agaagctacg gagacttatc gccgacgcga
     7501 acgcaaatgg tcgcgttctc ccaagcaccg tgcccgcggc aaagatgccc tctcgtgatc
     7561 ctagctgcgc atggtcccgc tgggcacaaa tgacatgaag tcgccgcggg tttttgcatc
     7621 gtccgtcttg ccggcagtct ggaggaaagt cacacggttg agtgtcggac attttgcccg
     7681 agccaaaaaa gcgacagatg cttgccaagg ccagggaggc tgaattaccg taaagagtta
     7741 gcgatctact ccaactcgct ggacgtcacc gatccgtaaa ctggaaagaa cggcaaccta
     7801 agcaagccat gtcagcatcg gacagacaga ccactcctgc cgacttcccc ttagaacagc
     7861 cgaggctcgc ttggttcttt ggcgtcctcc tggcggttgt ggtctggagc tactggaatt
     7921 ccctgagtga agccgccatc gcatggcggg gggcccagta ttctcatggt cttttcgttc
     7981 ccctgattgc tacctttttg ctctgggtaa gaagactcgg ggggaccttc gactggcaat
     8041 ggcaaacgac cacggagaaa tggcgtcttg ttgcagtggg gggtgtgctc gccctggcat
     8101 ggtttagcta catcgcctcg gagaggctgg gccaacaagc tgcgcccggg gaggccgttt
     8161 cgctgaccgc cgcgtcgggc ttggggttca tggcggccat gctgtatgcc acgggcttgc
     8221 tcacctgggc cacttttgcg gcggatagct ggcttcagca ctcgcttgaa agcgaaaaac
     8281 agcggcaaaa cgatgaggat aagactcatc ttcgagggga gttagcaggg gaagctcttg
     8341 ccagcgggac ccaaaccgcc ctggtgcgag gtcgctacgt aggattgttt atcctgattg
     8401 tcgctgtggt gttgcgactt gcatccgcct acgttggcct tgatgtggcc gaaatgtgga
     8461 cgttgccgct tggtcttttg ggcgcaggtg tttgcaccat cgggtggaga tcactccgct
     8521 ggagctggcc ggcagtggtc gttctgtttt ttgcctttcc tttgccgttc ggtgtggagc
     8581 gtacgatcct cgttcccctc cagggaatag ccgctcaagg cggtgcgtat ctattacaaa
     8641 gtttgggact agaagcatcc acggaggggg cacatttcat tcggctgggt gaatttcaac
     8701 tgggagtggt ggaacagtgt agtggcttgc ggatgactac ggtcttcatc acgttggtgt
     8761 tcgtttatgt tgtgctggtg cccacgccaa agtggcaaat ccctgtcctt ctattgagct
     8821 ccatccctat cgctttggtt gtgaacatcc tccgcattac ggtgacggga actctctacg
     8881 tttacggcag tcgggaattg gcccagcacg tcttccacga ttgggccgga tatctaatgc
     8941 cgctggctgc ggttgccttg ttgctgctcc ttcgtgttct gctagataac ctgtttatca
     9001 aagtggaaga acaagacgtg ccccttgccc agcgccttga accggcaaag aagggtagca
     9061 cgtag
//
LOCUS       DAOSUT010000162         8907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4161_length_8907_cov_10.065183, whole genome shotgun sequence.
ACCESSION   DAOSUT010000162 DAOSUT010000000
VERSION     DAOSUT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4161_length_8907_cov_10.065183"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLD05_12905"
     CDS             complement(<1..240)
                     /locus_tag="PLD05_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78389.1"
                     /translation="MARIAVDLLHRLRRSLALTPTLSQREREPRDSLLSTSCVMPVGV
                     HSREFGAFILLSIAQEWAPDRIRVRRAERVAAFRLS"
     gene            complement(453..1988)
                     /locus_tag="PLD05_12910"
     CDS             complement(453..1988)
                     /locus_tag="PLD05_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP78390.1"
                     /translation="MMGVHSIIASRLAFVWLRGLVCLSAVVTLPPLLAGDISQQAAST
                     KPNVVFILADDLGYTDLSCYGSKYYETPNIDRMAAEGMRFTDGYTAGPNCQPTRAALI
                     SGQYGPRTGVYTVGSIERFPWRTRTLRPVDNVEKLPLEKITLAQAMKQAGYATALFGK
                     WHLGEDAEHHPAARGFDEAIVSAGQHFNFKTNPPVTYPEGTYLADFLTDKAVDFIRRR
                     AKEPFFLCLHHFAVHSPHEAKADLIKRFEGKPPADGHKSPVYAAMIASVDESVGRVLA
                     TLKELGLDNNTLVIFTSDNGGVGGYERLGIKGGSITDNAPLRGGKGMLYEGGIRVPYI
                     FRWPGKIPAGAVCDVPIHSVDLYPTLLEIAGTPAPKDYVLDGVSYARLLQTGNKADFP
                     DRPLFWHFPGYLGAGQGTWRTTPVGVVRQGDWKLLEFFEDGHLELYNLREDIGEENNL
                     VSRYPDKVAALHALLKKWREEIQAPMPTPHTPEPSVQLQKTETRPGRGQGRRQQAKQQ
                     AQP"
     gene            2233..3693
                     /locus_tag="PLD05_12915"
     CDS             2233..3693
                     /locus_tag="PLD05_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOP78391.1"
                     /translation="MLKRLRRWWLRPCGARTVLTVAMPLVISFASWTVMNFTDRMFLV
                     WYDIGAVAAVLPAGMVQWTLAAFAMGLVSYVTTFVAQYQGAKRYHRVGPVLGQAYWTA
                     LLFIPPLMATIPFAYEMFSWIGHPPELAELEARYYQVAMLAVGAMLFSSVQAGFFTGR
                     GDTRTVMIVDTGAALLNLVLDYLWIFGHLGFPEGGVEGAAWASTVAQWAKVVWYGVLM
                     SGSRYDEYCLRDAWRFEGPLFRRLWRFGGPNGLQWLLEGAGFTGFLLLVGRLGEMATA
                     ATNLAINVNSVAFVPAIGLSIAVTTLVGQELGANRPRRAAVATWTAFHLAVIYTAVLA
                     IIYVAAPDVFLVAYGAGHDPAEFAPLRELVIVLLRFVAIYCIFDAMNLVFSGALRGAG
                     DTRFILLVGLVMSPIPTVLIWWGITSWSAGITFSWVVTTLWTCALGVIYLVRFLQGHW
                     RTIRVIEQTVIDEAESISELANLPVKAPAGILSEAD"
     gene            3888..4376
                     /locus_tag="PLD05_12920"
     CDS             3888..4376
                     /locus_tag="PLD05_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78392.1"
                     /translation="MTDFEKRLQKAIERGQQIRKQRAEAEIRRALTEQELRTRHSDLR
                     LQLTEYIEQCLKKLIEHLPGFTLESVFSERGWGSAVSRDELQWRRGESPQQRFSRLEL
                     VVRPFSEYYVLDLVGKATVRDKELFHRSFYQPLAEADLSAFKETVDRWVLEFAEAYTA
                     RG"
     gene            complement(4467..5114)
                     /locus_tag="PLD05_12925"
     CDS             complement(4467..5114)
                     /locus_tag="PLD05_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78393.1"
                     /translation="MDGYQRLRHRGLSGSLPSMDAAGLASLSARPTWLSRGRWATGLT
                     LAIALVVTGCHTPKVSHEAKMGPVYDRPFPLGQVTDAHWETMQTNAEAADFIFYDHEF
                     VGETAELTPLGRKHLLSVALRLEHVPFPIVVEESPRGENPQLDESRRQTIINQLAQLG
                     LDRTMVEARTVVAPAFTEGLSAIEGEAAYYRTLMDGNYGVGSYGSLGRGFGGWRR"
     gene            complement(5191..7989)
                     /locus_tag="PLD05_12930"
     CDS             complement(5191..7989)
                     /locus_tag="PLD05_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOP78394.1"
                     /translation="MTRMKWLQEAQPLTVIRWAAFACAVIFSLGLAYGLWQTTGHFFV
                     QANEPGGQSSVPIRLEVGTVQPLSNAAPSSQPSAEEPLVKLSIQPTIKREINNAPRPA
                     QNGSSAPTSKPNDSKPNDQDEIWIESDKPQEQSAASNKAMGEIWVEEDQPGNSLPAVA
                     AVPTPEKPSKDLVEARPATASPQEQCRIVLGNSESTVLRIAKAHVVRPSVSESASNQP
                     VAKEPPVPSPWAGSLVLPENSPDVLRKSREITELYQTFTIGGTAIFVKRAESKRPLSS
                     GEVPLSPEPAAHDEVTLTSAEAQVGQGTKEEKEPAREAVVVGWEFPGQTTESPKVDST
                     TLAITSAKPVFSQRDRLAQRDVHWETMPIQRPIGLASPQPDGQEQDDQKSSSGVVGSS
                     RTPSSAATQETENKDRRVQPLVTSQNDVRSENTLVKVSEFSNQSVPSPRATLASQENQ
                     PQSNQNLVEVVPNESRGEEPSFEIIEESGELTVVVRRSKLLRTKVDVYRTAVVDSGIC
                     DVVQFTPREISILGKSQGATHVTFWFQDERYRPVTYLVRVTPDPEVEKQQTERYELLE
                     SHLAKLFPESKVDLVVVGDKLVVRGQARDAAEAAQIMAIIRGELVRARGVMEGQASDP
                     VGREAAGQALPAIQVINMLRIPGPPQVALKVKIAELNRTAARNFGVDMSLNFDQGKFL
                     IQSLLNLSSGGSASILGNFDGGDLKFGLHYLEEHGVVRLLSEPTLITLSGRPATFIAG
                     GEFAVPTTVGVNGVGAVTTDFRAYGAIITFLPTVLDKDLIRLEVAPEFSQLDTKNKVN
                     GTPGLRTRAVTTTVEMREGQTLAIAGLLDDSMTGNNQGDLPIFNQIFGRRSLSRNETE
                     LIILVTPELVHPMEPEEVPPLPGFDVTEPTNHEFYIKGHLEGRPTQEYDSTTWPRLRR
                     RYNAGGPARISGPFGHGE"
     gene            complement(8113..8610)
                     /locus_tag="PLD05_12935"
     CDS             complement(8113..8610)
                     /locus_tag="PLD05_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78395.1"
                     /translation="MITANYRKLAKAAVVGLVLCCGWTIASLAWGQEGLSSSPPESAS
                     AAAAGSTLATEQPCPVGDPCHCLCHHGHQGCLARYMSAVGGFNCSCRGSYKFPVPPQY
                     TYHWPGLYSQQLMTSYQSPYRYPLLKLPPWMEKRPNADVPQNGALMPLMPSPGEKHAP
                     SNSGG"
     gene            complement(8693..>8907)
                     /locus_tag="PLD05_12940"
     CDS             complement(8693..>8907)
                     /locus_tag="PLD05_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78396.1"
                     /translation="IAITTPHAGRSNSYARFTFSNEHSIMKGGSAGGYPHGNLGKICR
                     MYRPDPAVLELRQHALQKSLNAFTFF"
BASE COUNT         1750 a         2482 c         2607 g         2068 t
ORIGIN      
        1 tgacagccga aacgccgcca cccgttctgc ccgacgaacg cggattctat ccggtgccca
       61 ttcctgggca atggaaagaa gtataaaggc tccaaactcg cggctgtgaa ctccaacggg
      121 cattacgcag gatgttgata agaggctatc ccgaggttcc ctctccctct gggagagggt
      181 tggggtgagg gcaagcgatc gtcggagtcg atggagaaga tcgacagcga ttcgagccac
      241 cctcaccccc ttcccttcgc ccgccgaacg ggagagggga ggttgtggga taggccctaa
      301 gttacgggtg gccgttctcc cggcccttac cgcaggccgc gaggagtatt ctgaagcttt
      361 tcgagccgaa aatttaacgc aatccgcttt tgggccgcga ccacggcgtc gtttccacga
      421 tgtgtcgaac gggtccgtcg gtggctggtt ctttagggct gagcctgttg tttggcctgc
      481 tggcgacgtc cttgaccacg ccccggtcga gtctcggtct tttgcaactg aacacttggt
      541 tcgggcgtat gcggcgtcgg catcggggcc tggatttctt cccgccactt tttcaacagg
      601 gcgtgaaggg ctgcaacttt gtcggggtac cgcgagacga ggttgttttc ctcaccgata
      661 tcctcgcgaa ggttgtacag ttcgagatgg ccatcctcga agaactccag gagcttccaa
      721 tcaccctgcc gcacaacccc caccggcgtg gttcgccaag tgccttgtcc agcccccagg
      781 tacccaggga agtgccaaaa cagcggtcgg tcgggaaaat cggccttgtt gccagtttgt
      841 aatagtcgag cgtagctaac tccatcgagg acataatcct tcggggcggg tgtcccggca
      901 atttccagca gtgtgggata cagatccaca ctatggatag gcacgtcgca gacagccccc
      961 gcgggaatct tcccaggcca acggaagata tagggcaccc ggatgccgcc ttcataaagc
     1021 atccccttgc caccgcggag gggtgcgttg tcagtaatgc tcccaccctt gatgcccaaa
     1081 cgttcgtacc cgccaacccc gccgttgtcg ctggtgaaga tgaccaatgt attgttgtcg
     1141 aggccgagtt ctttgagagt ggcaagaact cgaccgacgc tttcatccac actggcgatc
     1201 attgcggcat acactgggct tttgtgaccg tccgccggtg gtttaccctc gaatcgcttg
     1261 ataaggtccg cttttgcttc gtggggcgaa tggacggcaa agtggtggag gcacaagaag
     1321 aaaggctcct tggcccggcg gcgaatgaaa tcgacagcct tgtccgttaa aaaatcggcc
     1381 aggtaggtgc cttcgggata cgtcaccggc gggtttgtct tgaaattgaa atgctgtcct
     1441 gctgagacga tcgcttcgtc gaagccgcgg gccgcaggat ggtgctcggc gtcttcaccc
     1501 aagtgccact tcccaaatag ggctgtggca taaccggcct gtttcatggc ctgggcgagg
     1561 gtgatctttt ccagcggcaa tttctcgacg ttatctaccg gccgtagcgt tcgggtccgc
     1621 cagggaaacc gctcgatgga tcccacagtg tatacacccg ttcggggacc atattgcccg
     1681 ctgataaggg cagccctcgt tggttgacag ttgggaccag ccgtataacc gtcggtgaag
     1741 cgcatccctt cggcggccat gcggtcgatg ttgggcgttt cataatattt gctcccgtaa
     1801 caacttaggt cggtatagcc caaatcgtcg gccaaaatga agaccacatt gggtttggta
     1861 ctcgctgcct gctgcgatat atcccccgcc aataagggag gaagcgtaac cacggctgat
     1921 agacaaacca agcctctcaa ccagacgaaa gccagtcggc tggctataat ggagtgaaca
     1981 cccatcattg ccacatctcc ctaattgagt tccgaattca ccccacattg tggggtggcc
     2041 tgttcaacga gcggtacagt caccggcaca ccaatggtca ttcgtgtatt gcgccgacac
     2101 gcgcgagcgc aggcaatttt ggtgggctgg ggtcagattt ggggcagggc ttaggaggtt
     2161 ggaaaccagt taccggggcg ggttatcacg gagttgccga ctgttgaaat tgcatcacgt
     2221 aggagggtct ctatgctgaa gcgactgcgt cgatggtggc tacgcccgtg tggtgcccgc
     2281 acggtcctca ccgtcgccat gccgcttgtg atctcattcg cctcgtggac ggtgatgaat
     2341 ttcaccgacc ggatgtttct cgtttggtac gacatcgggg ctgtcgcggc cgtcctgccc
     2401 gcaggaatgg tccaatggac gttggccgcc ttcgcaatgg gtttggtgag ctacgtcacc
     2461 accttcgtgg ctcagtatca gggggccaaa cgttaccacc gggtggggcc ggtcctgggg
     2521 caggcctact ggacggcctt actattcatt ccgcctctca tggcgacgat tccttttgcg
     2581 tacgagatgt tctcgtggat tggccatccg ccggagttgg cggagcttga ggctcgatat
     2641 tatcaggtcg ctatgctcgc cgtgggggcg atgctctttt cgtccgtcca ggccggcttt
     2701 ttcacgggac gcggcgacac ccggacggtc atgatcgtcg atacaggcgc cgccttattg
     2761 aatcttgtcc tggactatct ttggattttc ggtcatctgg gctttcccga gggaggagtg
     2821 gaaggagcgg cctgggccag caccgttgcc caatgggcca aagtcgtgtg gtacggggtt
     2881 ctgatgtctg gcagtaggta cgatgaatac tgcctgcgcg atgcctggcg gtttgaaggg
     2941 ccccttttcc gtcgtctttg gcggtttggc gggccgaatg ggctacagtg gcttttagag
     3001 ggggccggtt tcaccggatt tttgcttctc gtgggacggc tcggtgaaat ggccacggcg
     3061 gccacaaatc tggcgatcaa tgtcaacagt gtggcctttg tgcccgctat cgggctgtca
     3121 attgccgtga cgacgttggt tggtcaggag ttgggggcca atcgaccgcg gcgggctgcg
     3181 gtagccactt ggacggcctt tcacctggct gtgatctaca cagcggtttt agcgattatc
     3241 tacgtggctg cgcccgatgt cttccttgtt gcctacggcg ccggacacga tcccgccgag
     3301 tttgccccat tacgggaatt ggtgatcgtt ctactccgct ttgtggcgat ttactgcatc
     3361 tttgacgcga tgaatctcgt cttttccggg gcgttgcggg gtgccggaga tacgcggttt
     3421 attctgcttg tgggtctggt gatgtcgcct attccaacgg ttttgatctg gtggggaatc
     3481 acgtcctgga gcgccgggat caccttctcg tgggttgtca ccaccttgtg gacctgtgcc
     3541 ctaggcgtaa tttacctcgt gcgattcctc cagggccact ggcggacaat tcgggtcatt
     3601 gaacagaccg tgatcgacga agcggagtca atttccgagc tggccaattt gcctgtcaag
     3661 gctcccgccg gtattttgag cgaagcagat taatgccttg ggggcaggtg tattgggaca
     3721 ccccaaaggc atttgaaagt ttcgagttgg agcacttggc ttcttaccga cgccaggcag
     3781 cattccccgg accagttatg ataaaattga tggggttgtc gcctaagtag ccgggatcgt
     3841 ggctcatcct tgaaatggaa cggtcgcgcc gaggtattta tcgtcccatg accgatttcg
     3901 aaaaacgatt gcaaaaagcc attgaacgtg gtcaacaaat ccgcaagcaa cgtgcggagg
     3961 ccgagatccg gcgcgccctg actgagcagg agcttcgaac acgtcatagc gatctgcggc
     4021 tgcaactgac ggagtatatt gagcagtgcc tcaagaagct gatcgagcat ctgcctggct
     4081 tcacattgga atcggtattt tcagaaaggg gctgggggag tgccgtcagt cgggatgaat
     4141 tacaatggcg acggggtgaa agcccccaac agcgctttag ccggctagaa ctggtagtcc
     4201 ggccattctc ggagtactac gttctcgatc tggtgggaaa agccaccgtc cgtgataaag
     4261 agctttttca tcgcagcttc tatcaaccgc tggccgaggc cgacctgtcc gcttttaaag
     4321 aaacggtgga tcgctgggtt ctggagttcg cagaggccta cacggcccgt gggtgaactg
     4381 ccaggtcacc aggccgttag gtcgaactgt gacgtcttca ttagatcgaa gtaagctgcc
     4441 ttttcggcgt tcctctgcgt gatgccttac cgccgccagc cgccgaagcc ccggccgagg
     4501 ctgccgtaag aaccaacgcc gtaattaccg tccatgagtg tccggtaata ggccgcctca
     4561 ccttcgatgg ctgacaatcc ttccgtgaag gcgggtgcca caacggtgcg ggcctcgacc
     4621 atggtgcgat caaggcctaa ttgggccaat tggttgataa tcgtctgccg acgactctcg
     4681 tccaattggg gattttcacc gcgtggcgat tcttccacca caatggggaa aggcacgtgt
     4741 tcgaggcgaa gggcaactga caggaggtgc ttacgaccca agggcgtcaa ttccgccgtc
     4801 tctcccacga actcgtggtc gtagaagatg aaatcggcgg cctcggcgtt ggtttgcatg
     4861 gtctcccaat gtgcgtcggt cacttgcccg agtggaaacg gccggtcgta aaccggtccc
     4921 atcttcgctt cgtgggagac cttgggcgta tggcaacctg tcacgaccag ggcaatggca
     4981 agagtgagac ccgtcgccca tctcccacgc gacagccatg tgggtctggc cgacaggctg
     5041 gccaatcccg cagcatccat actgggcagg ctaccactaa gtcctcggtg acgcaaacgt
     5101 tgataaccat ccatggcaat tgtcccgaat gtgagttgct tgatgatctt tcgcgatgat
     5161 ttgatctatt cctaagcgtg tgagactctt tcactccccg tgaccgaacg gcccggaaat
     5221 cctggccgga ccacccgcgt tgtagcgtcg ccgtaaccgt ggccaggtgg tgctgtcgta
     5281 ttcctgcgtt gggcgtcctt cgaggtgacc cttgatgtag aactcgtgat tggtcggctc
     5341 tgttacgtcg aaacctggca acggcgggac ttcttccggc tccatggggt gaaccaattc
     5401 aggtgtcaca aggattatga gctccgtctc gttgcggctc agacttcgtc gtccaaagat
     5461 ttggttgaag atgggcaggt cgccctggtt attgccggtc atagaatcgt cgagcaaacc
     5521 agcgatggcc aaggtttgtc cttctcgcat ttcgacggtt gtggtcacgg cccgcgtccg
     5581 caagcccggt gtgccgttga ccttgttttt tgtatcaagc tggctgaact cgggtgccac
     5641 ttccaaccgg attaggtctt tgtcgagcac cgtaggcagg aacgtaatga tggcaccata
     5701 ggcacggaag tcggtcgtaa ccgctcccac cccattcacg cccacggttg tgggcacggc
     5761 aaactcgcca ccggcgatga aggtagccgg gcggccactt agggtaatca acgttggctc
     5821 gctgagcagg cgtaccacgc cgtgttcttc cagatagtgc aggccgaact ttagatcccc
     5881 tccatcgaag ttcccaagga tcgaggcact gccgccggac gaaaggttta gtaacgactg
     5941 aatgagaaac ttcccttgat cgaaattcag gctcatatcg acaccgaagt tacgggcggc
     6001 cgtgcgattc aactccgcaa tcttaacctt cagtgccacc tgtggcggac cggggatccg
     6061 taacatattg atcacctgaa tcgccggtaa ggcctgacca gccgcttccc gtcctaccgg
     6121 gtccgaagcc tgtccctcca tcacgccccg tgccctcaca agctctccac gaataatggc
     6181 catgatctgg gccgcctcgg cggcatcacg ggcttggccg cgaacgacca gcttgtcgcc
     6241 gacgaccacc aaatcgacct tgctttcggg gaaaagcttc gccaggtgtg actccagcaa
     6301 ttcgtaacgc tcggtctgct gtttttcgac ttcagggtcg ggggtaactc gcacaaggta
     6361 cgtgaccggc cgatagcgct cgtcttggaa ccagaacgtc acatgagtag ccccctggct
     6421 tttgcctaaa atcgagattt cccgcggggt gaactggacc acatcacaga taccggagtc
     6481 caccacagcc gtgcggtaaa cgtccacttt tgtccgtagg agtttgctgc gacggacaac
     6541 gaccgtcagc tcaccggatt cctcgataat ttcaaaggat ggttcctccc cgcgagattc
     6601 attgggaaca acctccacca gattctggtt ggactgcggt tggttttctt gactggccaa
     6661 ggttgcccgc ggcgatggca ccgactgatt ggaaaactca ctgactttta cgagcgtgtt
     6721 ctcggatctc acgtcatttt gggaggtcac cagcggctga acgcggcggt ccttgttttc
     6781 agtctcttga gtcgctgccg aagacggtgt gcgacttgag cctacaaccc ccgacgagga
     6841 cttctgatcg tcttgctcct gcccgtcggg ctgcggactg gcaagcccga taggcctttg
     6901 gataggcatt gtctcccaat gaacatcgcg ctgggccagc cgatcccgct gagaaaacac
     6961 gggcttggcg gacgtgatgg ccagcgtcgt ggaatcgact ttgggagact cagttgtttg
     7021 ccccgggaat tcccatccga cgaccacggc ctcacgggcg ggttcctttt cttcttttgt
     7081 cccctgcccg acttgcgcct cggccgaagt taatgtcacc tcatcgtggg ccgcaggttc
     7141 cggggacagg ggaacttcgc cggacgaaag gggccgcttc gattcagccc gctttacaaa
     7201 gatagctgtc ccgccaatgg tgaaagtttg atatagctca gtgatctccc ggctcttgcg
     7261 gaggacatcg ggcgagtttt cgggtaaaac aaggctgcct gcccatgggc tgggaacagg
     7321 gggttctttc gccaccggct gatttgaggc cgattcactg acggacggac gaaccacatg
     7381 ggccttggcg attcgtaata cggtgctctc tgaatttcca aggacaatgc ggcactgttc
     7441 ctggggggag gcagtggcgg gacgggcttc caccaagtcc ttgcttggct tttcgggagt
     7501 tggtaccgcg gcaacggctg ggaggctgtt gcccggttgg tcttcttcga cccaaatctc
     7561 gcccatcgct ttgttcgagg ctgccgactg ttcttgaggt ttatccgact cgatccagat
     7621 ttcatcctgg tcatttggct tgctgtcatt tggcttgctc gttggggccg atgagccgtt
     7681 ttgcgctggg cgtggtgcgt tgttgatttc tctcttgatg gtcggttgaa tcgacaattt
     7741 gacgagcggt tcctctgccg atggctgact cgaaggtgct gcgttactta acggctggac
     7801 ggttcccacc tcaagacgga taggaacgct actttgccct ccgggttcgt ttgcttgaac
     7861 gaaaaaatga ccggtggtct gccacaagcc ataagccaat cctagcgaaa agatcaccgc
     7921 gcaagcgaac gctgcccagc gaatcaccgt taacggctga gcctcttgga gccacttcat
     7981 ccgtgtcatg gtctcatcct tgatatcaaa cctgaccgac cttggtcaga gaaagttgac
     8041 tctcctctgg tggaatcggt ttcgacttac cgactcagcg tcatactccc ttgtgctgtc
     8101 ttctcgcaaa aactacccgc ccgagttact tggagcgtgc ttctccccgg gtgagggcat
     8161 caatggcatt aacgctccgt tttgagggac gtccgcgttc ggcctttttt ccatccacgg
     8221 gggtaacttg agcaaaggat agcgatatgg gctttggtag gatgtcatca actgttggct
     8281 gtacagaccc ggccagtggt aggtgtattg cgggggaacg gggaacttat aacttccccg
     8341 acaactgcaa ttgaagccac ccaccgccga catatatcgt gccaaacagc cctgatggcc
     8401 gtgatgacaa agacaatgac aagggtcgcc aaccgggcaa ggttgctcgg tggccaaggt
     8461 cgaaccggcg gcggccgcac ttgcgctctc cgggggagag gacgaaaggc cctcttgacc
     8521 ccaagccaga ctggcgatag tccaaccaca acacaacaca agccccacaa ccgccgcctt
     8581 ggcaagcttt cgataattag cggtgatcac ggccacctcc gggggtgaaa tcccccacta
     8641 gcggggacga ttctgactgc tgtacgaact gtgtgactgg taacgactat cgtcaaaaaa
     8701 acgtgaaggc gttcagcgat ttctgtaacg catgttggcg taattccaaa actgctggat
     8761 cgggtcggta cattcggcat atcttgccta gatttccgtg cggatagcca cccgccgaac
     8821 cgcctttcat gatggaatgc tcgttggaga aggtaaagcg ggcataagag ttgctcctac
     8881 cagcgtgcgg cgttgtgatc gcaattg
//
LOCUS       DAOSUT010000163         8907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4162_length_8907_cov_9.197131, whole genome shotgun sequence.
ACCESSION   DAOSUT010000163 DAOSUT010000000
VERSION     DAOSUT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_8907_cov_9.197131"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLD05_12945"
     rRNA            <1..953
                     /locus_tag="PLD05_12945"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1055..1162
                     /gene="rrf"
                     /locus_tag="PLD05_12950"
     rRNA            1055..1162
                     /gene="rrf"
                     /locus_tag="PLD05_12950"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1910..2293
                     /locus_tag="PLD05_12955"
     CDS             1910..2293
                     /locus_tag="PLD05_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78397.1"
                     /translation="MPRDLAVEWLKSAIKDAEKVLPQNAARAQLQLLVERLRFLQTEG
                     FNADLPLKRLQGKFRKLSELRVKGGALGKSNIRVFFSVIENRVVILGVTKKEKEGALN
                     YVYETMLQRLEDYELRCASKERRQS"
     gene            2290..2778
                     /locus_tag="PLD05_12960"
     CDS             2290..2778
                     /locus_tag="PLD05_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOP78398.1"
                     /translation="MSTTQMHGLDQELSRVLMLISQIREKLPARKRRTLDVFLDKWLE
                     LAKVDHQDEDQRETMEAVVEGIVEIVSGADVSTVPSLVDPNSEEVKKAKAWFAKLGTA
                     IRKRRLEKKMTQKALAEKIGLSQGALSRIERGQLAPTHLTVQRIAQVLGVKPNDIDPS
                     LE"
     assembly_gap    2786..2885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2898..2970)
                     /locus_tag="PLD05_12965"
     tRNA            complement(2898..2970)
                     /locus_tag="PLD05_12965"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2934..2936),aa:Glu,seq:ctc)
     gene            3651..4766
                     /locus_tag="PLD05_12970"
     CDS             3651..4766
                     /locus_tag="PLD05_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78399.1"
                     /translation="MARDRVRVAIIGLGFGAEFIPIYQRHPQAEMYAICQRNQERLHQ
                     VGDIFGVPVRYHRYEDVLADPNVDFVHINTPIPDHAWMSIEALKAGKHVMCTVPMATT
                     IDECRKIVELVHETKLKYMMAETVVYSREFLFIKEMYQKGELGRIQYLQASHTQDMDG
                     WPDYWQRMVPMHYATHVVGPCLGLLNGRAEYVSCFGSGRVRQEIWEKSGSPFAIESCH
                     IKIKDSDVVAHIWRFLYDTARQYRESFDVYGSRASFEWTLVEGEPHILHTAKKPEPEI
                     PSKIRVPDYAHLLPEPIRLFTQAIHDPQHRSFIQGAGHGGSHPHLVHEFVSALIEDRD
                     PWPNAVQSANWTCVGICAHQSAMEGGRIIPLPDFTLT"
     gene            5208..5771
                     /locus_tag="PLD05_12975"
     CDS             5208..5771
                     /locus_tag="PLD05_12975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOP78400.1"
                     /translation="MRSPLQKSLSEHVGWVVLLTAIVGSTVAFANNSAQPTPTPPGHL
                     RPRAVDISLSQNGVFSGKVVDAQARPVEKAEILFRQSGKQVAKVSTSRNGDFDVTGLT
                     PGVYQIVVLPDRALTVRVWNKRSAPPVARPELLIVIGDDTIRGQRRIGELLPLENTVV
                     VGGMVAAAIAIPIAIANSSDDTEPVSP"
     gene            6190..7473
                     /locus_tag="PLD05_12980"
     CDS             6190..7473
                     /locus_tag="PLD05_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78401.1"
                     /translation="MQVSKTQLSRRRFLRTVMLGSGVAVPMLVSPRAMGFAGLAPASE
                     RIILGAIGIGNRGTYVLACFLQENDVECVAVADVKLFRREQAKRMVDERNGNTNCKTY
                     RDFRELLDRPDIDAVLIATGPNWHWLASVLAAKAGKDVYCEKPCTKNIMQSLSLAETF
                     RRTGRVFQAGTQRRNLPHFAFAVELAQKGRLGKLHTLYAHPAGMSTSMSGWAPPEPEP
                     NKEEIDWDLYLGPAAWRPFNKRFLDGFNFEKGGGLVGGGVLEWGSHCVDLCQWANQAD
                     HTAPVEYFPPENGQVSAVYANGVRLILRETGWLPLGSCPVRFEGDKGWVEAGDSGKLA
                     LSSPALLAGRKVAEIGGYPATFHVRDFLDCVKTRGKPKANADVACHSHITCHAVNIAI
                     FLNRKLRFDPVKYEFIGDDEANRLRGEALREPWRV"
     gene            7556..>8907
                     /locus_tag="PLD05_12985"
     CDS             7556..>8907
                     /locus_tag="PLD05_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78402.1"
                     /translation="MRSLKCMIIVLGTVLLLTAPCWAQQQPAKEADLIALLQSDGPKA
                     EKALACKKLAVWGTEASVPALAAYLNDPELASWARIALEAIPGDAPNQALRASLGQLK
                     GRLLVGAINSLGVRRDEGAVAMLAEKLTDADADVAAAAATALGHIGGPQAVAVLTPAL
                     NTSTGIVKNAVAEGLLFCADRFLAEGKKQEAREIYDAVRKADVPKPRRVEATRGAILA
                     SGKEGLDLLAECLASNDKDFFAIGLSTARELKGAEVSKVLVGQLGPIQKGEEKKPALL
                     TIKKAEYGAGDRWADVTQAVAAAVRNNTLRITASNALAGDPAPGVVKQLRMVYAIGGQ
                     EQTIAVKENDSVVLGESVAPADPRQVAILEALGDIGDPVGIEAIVKAARDGSWGLRLA
                     AIRILGRVGDAQAVPVLVTAAAEGGELAQAAAEGLIQLPGSDVDAAIVEALRTAQGPV
                     R"
BASE COUNT         1937 a         2333 c         2579 g         1958 t
ORIGIN      
        1 tgtcgggtaa gttccgacct gcacgaatgg tgtaacgatc tgggcactgt ctcaacagcg
       61 agctcggtga acttgtggta ccggtgaaga cgccggttac ccgcactggg acggagagac
      121 cccatgcacc tttactgcag cttattattg ggtctgattg catcatgcgt aggataggtg
      181 ggagactgtg aagcggagct tgtgggcttc gtggagtcgc cggtgaaata ccacccttga
      241 tgcagttgga ttctaacctg gcagagtgga tctgcaggga cagtggtagg tgggcagttt
      301 gactggggcg gtcgcctccc aaaaagtaac ggaggcttac aaaggtcacc tcagcacggt
      361 cggtaatcgt gcgtagagtg caaaggcata aggtggcttt actgtgagac cgacaggtcg
      421 agcagttgcg aaagcaggtc ttagtgatcc ggcggttctg tgtggaaggg ccgtcgctca
      481 acggataaaa ggtacgctgg ggataacagg ctgatcttgc ccaagagtcc acatcgacgg
      541 caaggtttgg cacctcgatg tcggctcatc gcatcctggg gccggagaag gtcccaaggg
      601 tttggctgtt cgccaattaa agcggtacgt gagctgggtt cagaccgtcg tgagacaggt
      661 cggtccctat ctaccgtggg cgcacgaggc ttgaggaggg tcctccttag tacgagagga
      721 cttggaggga cgcacctctg gtgtaccagt tgtcgcgcta gcggcacggc tggatagcta
      781 tgtgcggaaa ggataagcgc tgaaagcatc taagcgcgaa gccctctcca agatgaggcc
      841 tcgtagggat tcgtcccgaa agccccctgg aagactacca ggttgatagg ccgggtgtgt
      901 aagcgcagca atgcgctaag ctaaccggta ctaacgggcg aaagcttggc cacatgtatc
      961 aaacgctccc cacgcttggc caatgatcca ccaaagaata caaccgcccc ctccctctga
     1021 tcgatgtccg tctttcccga cggcatcggt cctccccggt ggccatacct ggaaggaaac
     1081 acccgttccc attccgaaca cggccgttaa gcttccaggg ccgatggtag taccaaaagt
     1141 gcgagagtag gtgctgccgg gactaataca caacagcccc cgacggattt ccgccggggg
     1201 ctcttttttt tcgtaccaag agcttatgag cttatcccat aatcttccct cgcccgttcg
     1261 gcgggagaag ggtcggaggt gaggctggcc cgaatcgctg ccggttttct caatcgattc
     1321 cgacgttcgc ttgccctcac cccaaaccct ctcccaaagg gagaggggga tttttggggt
     1381 aggctccaac ttgggcttaa gcttccatcg aactccgtgg actcctcgcc aatcgcaccg
     1441 acggtcctcg tgccgttccc tacacgattg ttcttgcccg aaaaagacaa ggcctttttc
     1501 ggttagacag cgcccgcgcc cccgtgtgag aaaatgaatc ccctgcttga agcacgagtg
     1561 aactattgaa ctataagagg cccaccgtca gcaggcaaaa gacagcccca tcggtaatcc
     1621 caataccccc tacgggagtc gaacccgtgt tttctggatg atgatcggat gtgccgtgag
     1681 ctgagtcggt tgtccgtggc tcgatcctac ccttacttgg ctgctcgccc cgtttaggat
     1741 aatgttcgtt gacaagcgtt gatacctgtt aatacattga gccacattgg cagtcatcgc
     1801 cccccaagac tgaggacaac atatttacct cccatacatg cgtttgacaa ccggtaatat
     1861 gcgcggtaat attacatgcc ggcaccagtg acgaacggga actgcgtgaa tgcctcgcga
     1921 tttagcagtg gaatggctca aatccgccat aaaggatgcg gaaaaggttt tgccccaaaa
     1981 cgcggcccgg gcgcaattgc agctgttagt tgagcggctc cgttttttgc aaacggaagg
     2041 attcaacgcg gatttgccgc taaagcgttt acagggcaaa ttccgaaagt tgagcgaatt
     2101 acgggtgaag gggggagcac tggggaaatc taacattcgg gtgtttttca gcgtaatcga
     2161 gaatcgagtt gtcattctgg gggtgacaaa aaaggagaaa gagggcgcac tgaactatgt
     2221 ttatgagacg atgttgcagc gactcgaaga ctatgagttg cgatgtgctt caaaggagcg
     2281 gcggcagtca tgagcacgac tcaaatgcac ggtttagatc aggaattgtc cagagtccta
     2341 atgctaatct cccaaattcg tgagaaattg cccgcgcgca aacggcggac tctcgatgtt
     2401 ttcttggaca agtggcttga gttggccaag gttgatcatc aggacgagga tcaacgtgaa
     2461 accatggagg ctgttgtcga gggcatcgtc gaaatcgtta gcggcgcgga cgtttcaaca
     2521 gtcccctcat tggtagatcc aaacagtgaa gaagttaaaa aggcaaaggc gtggtttgcc
     2581 aagttgggga ccgctataag aaagcggcgg ctcgagaaga aaatgacaca aaaggccttg
     2641 gctgaaaaga tcgggttaag ccagggagct ctttcccgca ttgaacgagg acaattagca
     2701 cccacgcatt tgacagtgca acgaattgcc caggtgctag gggttaaacc gaacgatatc
     2761 gacccaagct tggaatgatc gtaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnncacaa cacccattac cccctacggg agtcgaaccc gtgttttcgg actgagaatc
     2941 cgacgtcctg ggccactaga cgaagggggc ttacctattc accacttatc gaaacttatc
     3001 ttaacgcttt agctcggttt gtcgagcgat atctgtcaag cgacatcgct tagtatcgac
     3061 ggacatgcgc aaatccataa ctttttgccc gccgttctag ctacccttaa tttacgcacg
     3121 gcttcggctt tttcaagtag gtcctcccgc gattggctca ttgccactcg caacgtcacg
     3181 ccagcggttc gagctgaaca tgccgtgtgc caatgattgg ccaagaccaa tattacacat
     3241 gcggcacggc ggtggcacct tttaccgtag agtctctcac gcgcggcgaa gcgatccaca
     3301 acaacacttc gggcgacttg gtggggtcaa ggcttggtag caagcggctc ctctcaaagt
     3361 cggcttcctg gccactatcc gcccaacgtg atgcaggctt gccaccgttg accgtcaccg
     3421 cacagcaatc gtccccaatt gattgcacgg cgcacccggt tcgcatacaa tcgacgcagc
     3481 cgtcggggaa agacttcccc aagtttcagc aagcgtgtcc ggcttcctta ggtttgcatc
     3541 aacggctctg gctgtgacaa tttgctgaga cgatttagcg ggaacgccgt tctgtttaga
     3601 gcatggtgtg gaagggcatc aaccctgttc caacaatagg agactcgtcg atggccagag
     3661 accgtgttcg agtggcaatt attgggcttg ggtttggagc cgagttcatc cccatctatc
     3721 aaaggcaccc gcaggccgag atgtacgcca tttgtcagcg taaccaggaa agactgcacc
     3781 aagtgggcga cattttcggt gtccccgtcc gctatcatcg gtacgaggat gttctcgccg
     3841 accccaatgt ggactttgtc cacattaaca cgccgattcc tgaccacgct tggatgtcca
     3901 tcgaggcact caaggcggga aagcacgtca tgtgcaccgt gccgatggca accaccattg
     3961 acgaatgtcg gaagattgtg gagcttgttc acgagacgaa actcaagtac atgatggccg
     4021 agacggtcgt ctattcccgc gaattccttt tcatcaagga gatgtatcaa aaaggagaat
     4081 tgggcaggat ccagtatctg caagcctcac acactcagga catggatggt tggcctgatt
     4141 actggcagcg gatggtcccc atgcactatg ccacgcacgt cgtggggccg tgtttgggcc
     4201 tgctcaatgg gcgggctgaa tatgtatcgt gtttcgggtc agggagggtt cgtcaggaga
     4261 tttgggaaaa atcgggcagt ccgttcgcca tcgaatcgtg tcacatcaag atcaaagact
     4321 cggatgtggt ggcccacatt tggcggttcc tctacgacac cgctcggcag tatcgtgaga
     4381 gcttcgatgt ctatgggagc agggccagtt ttgagtggac tttggtcgag ggtgaaccgc
     4441 acatcttgca tacggccaaa aaaccggagc cagagattcc cagcaagatc agggtacccg
     4501 attacgccca tttgttgccg gaacccatcc gcctattcac gcaagcgatt cacgatccgc
     4561 aacatcgttc ctttatccag ggggcgggtc atgggggatc tcacccccat ttggtccacg
     4621 aatttgtttc ggccctcatc gaggatcgcg acccctggcc gaatgccgtg cagtcggcga
     4681 actggacatg tgtgggcatc tgcgcgcacc aatcggccat ggaaggagga cggatcatcc
     4741 cgctgcccga cttcacactc acctaaccgg tggctttcgt gaaggtccgg ttttggtcaa
     4801 gatgatgcga aaccgtttta atggcccgca tcctgttatg taggatttcc atggttttga
     4861 acggtctagc agactaagcc ccttctcgat tgcaaagccg tggtgggccc gctgatcgga
     4921 gttctagcct ccgcggccgg tggcggctta gcacagaatc ggcaaactgg gtccggatct
     4981 tgcccatttc taatggcggg tcttgcgtag tcccctattc cctcccacct tatcccgaca
     5041 acggtttttg gtgtgcgaat tgctcttgat ggaaccgacc tgggttgcta aggttcgccc
     5101 ggttttcgac acagttacga acgaatcagg ggaaccgtga aggcgattcc ctagcctaag
     5161 ccattggcct tgtagctaac gttccgaagg gagtatggag gtttgtgatg aggtccccac
     5221 tgcagaaatc cttgagcgaa cacgtcgggt gggttgtgct gctaacagca atcgttggct
     5281 caacggtggc ctttgccaac aactctgcac agccgacacc aactccgcct ggacatttgc
     5341 gacctcgcgc tgtggacatt tcgctgagtc aaaacggcgt tttttccgga aaagtcgttg
     5401 acgcccaagc tcgaccggtt gagaaggccg aaattctgtt tcggcagtcc ggcaagcagg
     5461 tggctaaggt ttctaccagt cgcaacggag acttcgacgt aaccgggtta acgccaggag
     5521 tctatcagat cgtcgtactg ccggaccgcg ccctcacggt gcgtgtttgg aacaaacggt
     5581 cggccccgcc ggtagcacgt cccgagctgc ttattgtgat aggcgacgac acaattcggg
     5641 gacaacgccg aatcggtgaa ctcttgcccc tagagaatac ggtggtggtc ggcggcatgg
     5701 tggcagcagc cattgccatc ccaattgcga tcgctaactc tagcgacgat accgaacctg
     5761 tcagcccgtg agtatcctcc ttccgcgttc cccagactga taacacgtcg aagtcgagag
     5821 ctcatcatgc gccctcagtt tgatttcgaa atcgccccat tccccttatc ggaggtcctc
     5881 tgacagtcct gatcgcatgt ccaaaaagga aatcagcggc tagaacgaat ttcaatgttg
     5941 cgccggcgaa gcacttttgc gttcccggcg taacacttga caacggtgtc gtttcagacc
     6001 atcatcaatc gcggactgta accattcact gggttggtct ttacctccct cgtgcaagtg
     6061 ttcccaccct tttttgggtt gcacgctggt gttccgggat gagatggcat gtgggcctgt
     6121 gttggtaacc atggcccatg tttggtcttt gagtgcatct ttatgcaaga ctaaaaaagg
     6181 agagttggca tgcaggtttc caaaacacag ctttcccgcc ggcggttcct tcgcacggtg
     6241 atgctgggga gtggagtcgc cgtgcccatg ttggtatcgc cgcgtgcgat gggctttgcc
     6301 ggacttgctc cggccagcga gcggattatc ttgggggcca ttggcattgg taaccgggga
     6361 acgtacgttc tcgcctgttt tctccaggaa aacgacgttg aatgtgtggc cgtagctgac
     6421 gtcaaactgt tccggcggga acaggccaaa cgaatggtcg atgagcgaaa cggcaataca
     6481 aattgcaaga cttatcgaga cttccgtgaa ttgctcgatc ggccggacat cgacgcggtt
     6541 ctaattgcca ccggcccgaa ctggcactgg ttggcctcgg ttttggcggc gaaggcaggc
     6601 aaggacgtgt attgcgagaa gccttgcacc aagaacatca tgcagagttt gtcgttggcc
     6661 gagacgttcc gtcggaccgg tcgcgtcttt caagcgggga cgcagcgacg gaatcttccc
     6721 catttcgcat ttgcggtgga gttggcccaa aaaggtcggc tgggcaagct gcataccctt
     6781 tatgcccatc ccgcgggaat gagtacgagc atgagcggtt gggcaccgcc ggaacccgag
     6841 ccgaacaaag aagaaatcga ttgggacctt tatcttggcc ccgcagcgtg gcggcctttc
     6901 aacaagcggt ttctcgatgg gtttaacttt gaaaaaggcg gtggccttgt cggcggcggt
     6961 gttctcgagt ggggttccca ttgtgtcgat ctctgccaat gggccaatca agcggatcat
     7021 acggccccgg ttgagtattt cccaccggaa aacggccagg tttctgccgt ttacgccaac
     7081 ggtgtgagat tgattctgcg ggaaaccggc tggttgccgt taggctcttg ccccgtgcgg
     7141 tttgaaggcg acaaaggttg ggtcgaagcc ggtgacagcg gcaaactggc tttaagctct
     7201 ccagcactgt tggcaggtcg caaggtggcc gagatcggcg gctatcctgc aacgttccac
     7261 gttcgtgatt tcctcgactg cgtgaaaaca cggggcaagc caaaggcaaa cgctgatgtt
     7321 gcttgtcatt cccatatcac gtgccacgcg gtaaacattg ccattttcct caaccgaaaa
     7381 cttcgctttg atcccgtgaa gtacgaattt atcggggatg acgaagcgaa tcgcctgcgc
     7441 ggcgaggcac tccgcgagcc gtggagagtt tgatgggtgt tgaacgttgg gtcgtaattt
     7501 cctcacttag ccaaatcgat aacatctgag agttttccca gtcggaggat aaaccatgcg
     7561 aagtttgaaa tgtatgatca tcgtgctggg cactgtgttg cttttaactg ctccatgctg
     7621 ggcccaacag caaccagcga aagaagccga cttgattgcc ctcctgcagt cggatgggcc
     7681 aaaggccgag aaggccctgg cctgcaagaa gctggcggtc tgggggactg aggcatctgt
     7741 tccggccttg gctgcctatt taaatgatcc tgagttggcc tcgtgggcga gaatcgccct
     7801 ggaggccatc ccgggagacg ctcccaatca agccttgcgg gcaagcctcg ggcaattgaa
     7861 aggtcggctc ctggttggag ccatcaactc ccttggggtg cgacgtgatg agggagccgt
     7921 ggccatgttg gccgagaagc tcaccgatgc ggatgccgac gtcgcggcgg ctgcggcgac
     7981 agcgttaggg catattggag gtcctcaagc cgtcgcggta ctgacccccg ccttgaatac
     8041 ttcaacagga atcgtcaaga atgcggtcgc ggaaggcctg ttgttctgtg ccgaccgatt
     8101 cctcgcggag ggcaaaaagc aggaagcacg agaaatctac gatgctgtcc gcaaggccga
     8161 tgttcccaag ccgcgtcgtg tggaggccac ccgaggggct atcctcgcca gcgggaagga
     8221 aggtcttgac cttttggccg agtgccttgc ctccaatgac aaggacttct tcgctatagg
     8281 tctttccacg gcaagagagt taaaaggggc agaggtgtcc aaggtcttgg ttggacaact
     8341 tggtcccata caaaagggcg aggagaagaa gccagcgctg ttgacgatca agaaagccga
     8401 atatggagcc ggggatcgct gggccgacgt gacacaggct gtggcggctg cggtgcgcaa
     8461 caatacgctt cgtatcacgg cttccaacgc cttggcggga gaccccgctc ccggtgtggt
     8521 gaaacagttg cggatggtgt acgccattgg ggggcaggag cagaccattg cggtcaagga
     8581 gaacgactcg gtggttctcg gggagtccgt ggcaccagct gacccacggc aagttgccat
     8641 tctggaggcc ctgggcgata tcggcgatcc cgtggggatc gaagcgatcg tcaaggccgc
     8701 ccgagacgga agctggggct tacgactggc ggcgatccgc atcttgggac gggttggcga
     8761 cgcccaggca gtccccgttt tagttacagc cgctgccgag ggtggtgagt tggcccaagc
     8821 cgcagccgag ggtctcatcc agttgccggg atcggatgtc gatgcggcga ttgtggaggc
     8881 cttgcgcacg gcccagggtc cggttcg
//
LOCUS       DAOSUT010000164         8849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4186_length_8849_cov_8.722766, whole genome shotgun sequence.
ACCESSION   DAOSUT010000164 DAOSUT010000000
VERSION     DAOSUT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4186_length_8849_cov_8.722766"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1176
                     /locus_tag="PLD05_12990"
     CDS             214..1176
                     /locus_tag="PLD05_12990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78403.1"
                     /translation="MKPFSRPAFTLVELLVVIAIIGLLIALLLPAVQMAREAARRSEC
                     LNHLKQIALACHNFHDSQRRFPPGYLGPKPQAPVPPYPGQWTSVFAFLLPFLEQASLK
                     EESDADASSFNGVSLYHDDRVGESFWLRPKAFEAAKKQPQVFLCPSDNARKIERPIVL
                     LHFHYVSPYVTLVAASFDDNRGDELGRTNYLGSGGYMGVTGIASSDAYRGVFWNRSQE
                     TFSSISDGTSNTLLFGEAMGGTQSQRRAYSWFGCGVMASAWGLERSADNNTNWGWWQF
                     TSRHPGVVQFALADGSCRPISHTIDRDTLIYLSAVQDGQNVAPP"
     gene            1494..2495
                     /locus_tag="PLD05_12995"
     CDS             1494..2495
                     /locus_tag="PLD05_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOP78404.1"
                     /translation="MARDYYKILGVPRNATKAEIEKAYRRLARKYHPDMNPNDASAKE
                     KFQEVQEAFEVLSDPQKREMYDRYGASFQYAGTGAGGAGPGYGGFYETHPGGFTFTFD
                     DFDLNRFFQERYRDDFLGGRPSGTGFEELFAQFQRAAGRARPDTGRAAYRGQDLHSEV
                     TIPFHVAVEGGKVDITVNRPGVGRETLAVMIPAGIEDGKKIRLKGKGAPGPAGGEPGD
                     LYLTVHVAPHRWFERKGNDLYVKVPVTIPEAIFGAKVEIPTPKGRIALRIPPGAASGK
                     KLRVPGYGVHPRDGTPGDLYAEVQIVPPPQVPESLKSSIEQLAREYPRDVRADLHWP"
     gene            2558..>2892
                     /gene="rnpA"
                     /locus_tag="PLD05_13000"
     CDS             2558..>2892
                     /gene="rnpA"
                     /locus_tag="PLD05_13000"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOP78405.1"
                     /translation="MGEFSFGREYRIRRQKDIDRVFARRCSVADDVIIIYGCENGQGL
                     PRLAIMASRKLGKSVVRNRWKRLIREVFRLNKHRIPRGVDYVVIPKPGATPDFRTLFR
                     TFPHLAKQL"
     assembly_gap    2893..2992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2993..3537
                     /gene="yidD"
                     /locus_tag="PLD05_13005"
     CDS             <2993..3537
                     /gene="yidD"
                     /locus_tag="PLD05_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOP78406.1"
                     /translation="PDFRTLFRTFPHLAKQLKRRLDRSAKREESGQATSVGVSSSFQE
                     KAEGRESSRETQLSRGLPQTEVDEATRDTTGPTTDRHDLGEDPPGVGFRGLRKITHPI
                     WRVLRLIDAAIGEVLILPVYVYRWTVSPLLGHCCRFEPSCSQYFIQAVRKYGAIVGSL
                     KGLARILRCHPWHPGGYDPP"
     gene            3985..5187
                     /locus_tag="PLD05_13010"
     CDS             3985..5187
                     /locus_tag="PLD05_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78407.1"
                     /translation="MSSKDYSPFTPGQPVPVEFFVGRSKEIDHIISAVRASTAGRLQV
                     LFLTGERGIGKSSLARLVRIIADRKYGVLGLHSFLGGVTSLDETVRRVFDRLLKESVG
                     KGWWAKIKEFFGDHIRQVDLFGVTVEFGAAEKELRRMVHDFAPALRNLLQKLRPEKKG
                     ILVVLDDINGLAASDQFANWLKSLVDEIATSSDPLPLCLMLVGLEDRRQSLVSLQPSL
                     ARVFDVVELRTWSDAETRDFFVNAFSKVGIHVEEEALKILVRFTGGFPVMAHEIGDAT
                     FNEDTDNRITREDALQGVVRAADIVGRKHVEPQVFTAIRSPRYRAILRKIASKPFEVE
                     FRRRDLWPLLKDDERKVFDNFLARMKKLGVVQANPEGGPGAYRFTNTLHYLYFWLEAA
                     RAKQSKEL"
     gene            5528..6637
                     /locus_tag="PLD05_13015"
     CDS             5528..6637
                     /locus_tag="PLD05_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOP78408.1"
                     /translation="MSAEARDSVPFFQRRERLRRRLSKDGLAGMLITKTENVRYLTGF
                     GGDDSYLLLWKDHEILISDSRFTEQIRQECPGLEAHIRRTGVSMHQAAANLINRAKLT
                     SVAVEASLPMATYKKLKAALKQTELVEAEDWIEQLRAVKDKTEIARIRKAVQYAERGF
                     AAVRATLRPEHSEKEIADLLEYQMRTLGARCSSFPTIVAVGERAALPHAIPSDRKVSE
                     NVFILIDWGADEGWYKSDLTRVLITGRISPKFAKVYQTVLRAQQAAIAAVRPGVSAQE
                     VDAAARQVIADAGFGRLFGHGLGHGVGLEIHELPRLGPNSSITLKPGMVITIEPGIYI
                     PDWGGVRLEDDVLVTRDGCEVLTRVPKDLEEVVVF"
     gene            6677..7246
                     /gene="accB"
                     /locus_tag="PLD05_13020"
     CDS             6677..7246
                     /gene="accB"
                     /locus_tag="PLD05_13020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOP78409.1"
                     /translation="MASDVPDNEGRDFPEVPDVFDLARVRELVELMRAHELSEIDLRE
                     GKRRIRLRRGPESVPAATGPAVPFPIFMPGSAPVPPPTPAGPVTPPPVREPEATSEAP
                     KQKEEENIVFIRSPMVGTFYAAPDPDSPPYVKVGDMVGPDTVVGIVEAMKVFNEIPAE
                     VSGQIVAILVENGEPVEYGQPLFKVDTKA"
     gene            7304..8656
                     /gene="accC"
                     /locus_tag="PLD05_13025"
     CDS             7304..8656
                     /gene="accC"
                     /locus_tag="PLD05_13025"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOP78410.1"
                     /translation="MFKRILIANRGEIALRVIRACREMGIETVVVFSEADRGSAYLEL
                     ADEAYCIGPPKASESYLRYDRVISAAEIGNAQAIHPGYGFLSENAHFAEVCRNCQIEF
                     IGPPHEAMALLGDKNAARALAKKVGVPIVPGSEGTVETETEAIRVAREIGYPVLIKAA
                     AGGGGKGMRIAVNETALKTAFQQAQQEAEAAFGNGAVYLEKYIEHPRHVEVQAIGDQY
                     GNVVHLWERDCTIQRRHQKLIEESPAPQLPQEVRQAICEAAVKLLKATNYYNAGTVEF
                     LVDRQFNFYFIEVNARIQVEHPVTEMVTGLDLVKLQIKVAAGEPLPFKQSDVEHRGVA
                     IECRINAEDPERQFRPSPGRIERMFVPGGPGVRFDSHVYPGYVVSPHYDSMIGKLIVH
                     RPTRAEAIAAMKKALEELRIEGIKTTIGLIQAILSHPTFVDARADTTFVERTWTFNQA
                     "
BASE COUNT         1895 a         2270 c         2614 g         1970 t
ORIGIN      
        1 cggggatcgc atccccggtc caacgccggc cacttacgcc ggcgttttcg tttcccctcg
       61 aaacacgcgg gcacttgacc cctccgcccg caccggcgct ctcgggagca gtcggctcag
      121 cgcggcatta aatcgtttgc cctcgtctca aaaacgacta tcattgaaaa gatgaggggc
      181 cctgggcttt gattgctcac aataccggca cacatgaagc ctttctcacg tccggcattc
      241 acgcttgtcg agctcttggt ggttattgcc atcattgggc tgcttattgc gctgcttttg
      301 ccggccgtgc aaatggcccg cgaggccgcc cgacgcagtg aatgcctcaa tcacctgaag
      361 caaatcgcct tagcttgcca caattttcac gatagtcagc gccgctttcc gccgggttac
      421 ttgggtccca aaccgcaggc accggtccca ccctatcccg ggcaatggac aagtgtcttc
      481 gcgtttcttc tccctttctt ggagcaggct agcctcaaag aagaaagcga tgcggatgcc
      541 agttccttca acggggtttc tctttatcac gacgatcgcg tgggcgagtc tttttggctt
      601 cgtcccaagg catttgaggc ggccaagaag caaccccagg tctttctctg cccctccgac
      661 aacgcacgaa agatcgagcg ccccattgtc ctactccatt ttcattacgt ttcaccgtat
      721 gtgacgttag tcgcagccag ttttgacgat aaccggggcg atgaattggg gaggaccaat
      781 tatctcggtt ccgggggcta tatgggggtg acggggatcg ccagcagcga cgcctatcgc
      841 ggggtctttt ggaatcggtc ccaggagact ttctctagca tcagcgatgg cactagtaat
      901 accctccttt tcggcgaagc gatgggtggt acgcaatcgc aacgtcgggc ctatagctgg
      961 tttgggtgtg gtgtgatggc cagcgcctgg ggattggaac gatcggccga caacaacacc
     1021 aattggggat ggtggcagtt tacctcccgc catccggggg tggttcaatt tgccctggcc
     1081 gacggatcct gccgtcccat cagccacacc atcgaccgcg ataccctgat ttacctttcg
     1141 gcagttcaag atggtcaaaa cgtagcgccg ccgtgacgtc ggttgcgctg aacggtgcct
     1201 agcccataac cgtggcacgg tttttgctta gaagactgtc cacgcttttg caagccggtt
     1261 attcggggac gcaagctgtc gaaattgccg aggggaggac gcgcgactgt catcttgggc
     1321 ggagctattg taacaaaacg aaagcatttt tctcatacga ttctggcggc agcttgcgtg
     1381 gcgataccgc cgtggcagtc ggcgcggtat tctgaaagga ggtcttgtcc ggccaggtct
     1441 gttgtcggca tcgctgtgaa gacaatccga gctaaccatc ggacgaaaac attatggcac
     1501 gcgattatta caagatcctt ggggttcctc gaaatgcgac caaggcggaa atcgaaaagg
     1561 cttaccgccg attggcgcga aaataccatc ccgacatgaa ccccaacgat gcaagcgcca
     1621 aggaaaagtt ccaggaagtc caggaggcat ttgaggttct aagtgacccc caaaagcggg
     1681 agatgtacga tcgttatggg gcatccttcc agtatgcagg gaccggggca ggtggagcag
     1741 ggcctggata cgggggattc tatgagacac accccggtgg gttcaccttt acgttcgacg
     1801 acttcgactt gaacaggttt ttccaagagc ggtatcgaga cgatttcctc ggcggtcggc
     1861 cctccggtac cggttttgag gagttgttcg ctcaattcca acgagctgcg ggcagggccc
     1921 gtccagacac ggggcgtgct gcttaccgcg gccaggattt acatagcgaa gtgacgattc
     1981 ctttccacgt ggctgtggag ggtggcaagg ttgatatcac ggttaaccgg ccgggagttg
     2041 gccgggagac tttggccgtc atgattcctg cggggatcga agacggaaag aaaatccggc
     2101 tgaaagggaa aggggcccct ggtccggcgg gtggagagcc tggcgatctt tacctcacgg
     2161 tccatgttgc ccctcatcga tggttcgaac ggaaagggaa cgacctctac gtgaaggttc
     2221 ccgttaccat tccggaagcc atttttgggg cgaaggtcga aatcccgaca cccaaaggcc
     2281 gcattgctct gcggattcct ccgggagcag ccagcgggaa gaaactccgc gtcccggggt
     2341 atggcgttca tccgcgagat ggcacgcccg gtgacctcta cgcggaagtg caaatcgtcc
     2401 cgccgcctca ggtcccggaa agtttgaaat cgtcaatcga acaattagcc cgcgagtatc
     2461 cccgcgacgt gcgggcggac ttgcattggc catgattggg gctgtccgat aacgacgaac
     2521 gggccgtttt gtaagtatcg aacgggaccg ggtcctaatg ggtgagtttt cttttggtcg
     2581 tgagtatcga atccgcaggc agaaagacat tgaccgcgtt ttcgcgcggc gatgctcggt
     2641 tgccgacgac gtgatcatca tctatggctg cgaaaatggc caaggcctgc cccgactagc
     2701 catcatggct tctcgtaaat tgggcaagag cgtggtcagg aatcgctgga aacgcttgat
     2761 tcgagaggtt ttccgcttga ataagcacag aatcccgcgc ggtgtggatt acgttgtcat
     2821 tcccaagccg ggggctacac cggatttccg cacgctattt cgaacctttc cgcatttggc
     2881 aaaacagctc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaccggat
     3001 ttccgcacgc tatttcgaac ctttccgcat ttggcaaaac agctcaaacg gcgactggat
     3061 cggtcggcaa aaagggagga aagcggccaa gcgacaagcg tgggggtgtc ttcctctttt
     3121 caggagaagg ccgaaggccg cgagtcttcc cgggagacgc agctttctcg cggacttcca
     3181 caaacggagg tggacgaggc aactcgtgac acaactgggc cgacgaccga ccggcacgac
     3241 ctgggggagg atccgcccgg ggtaggtttc aggggcctgc gaaagattac ccacccgatt
     3301 tggcgggttc ttcggttgat cgatgccgcc atcggcgagg ttttgattct gccggtttat
     3361 gtttaccgct ggacggttag cccgctgttg ggccactgct gccgtttcga gccaagctgt
     3421 tcgcagtatt ttattcaggc cgtgcgcaaa tatggggcta tcgtgggaag tttgaagggc
     3481 ttggcacgga ttcttcgctg ccacccctgg caccctgggg gttatgatcc gccgtgagat
     3541 ctcagagcga gccaggtggt agtctttagc gaaagtccaa aagtgccagg atggcccgga
     3601 aggcgatgac acctccctgt ataccgtata ccgttccggc cccccggctg cgttttacga
     3661 attagagcct gtcctgtaat ctcccctcac ccgttcggcg agagaggggt gggggtgaaa
     3721 gtggctcgaa tcgctgctgg tgttctcaat cgattctgac gctggcttgc cctcacccca
     3781 accctctcct ggagggagag ggggacttat tgggataggc acttagttgc agacaacggc
     3841 atcccttcca ctaggcgtgt gcttcgggaa ggtttcccat gtctcgtgaa cagttacttg
     3901 attgcgtttc cccaacggga caatacgatt atactgcccg atagtgagga gtggtgcatg
     3961 gttccgtgaa aaatggagcg caaaatgtca tctaaggact acagtccctt cacacccggc
     4021 cagcccgtac cagtcgagtt ttttgttggg cgttcgaagg aaatcgacca cattatctcg
     4081 gctgtgaggg cttctacggc aggaaggctt caggtgctct tcctaacggg agaaagaggg
     4141 ataggaaaga gctcccttgc gagattggtg cggattattg cagaccgtaa atatggagtt
     4201 ctcggactgc acagctttct tggcggtgtg acgtcccttg atgagacggt gcggcgggtt
     4261 ttcgaccgtc ttctcaagga gagtgtgggc aagggttggt gggcaaaaat aaaagaattc
     4321 tttggcgatc acatccggca ggtggaccta ttcggtgtca ccgttgagtt cggtgccgca
     4381 gagaaggaac tccgtcgcat ggtccacgat tttgcaccag cattgcggaa ccttctccag
     4441 aaattgcgtc cagagaaaaa gggcatcctg gtggtgttgg acgatatcaa tggtctcgct
     4501 gcctcggatc agtttgccaa ttggctgaag agcctcgtgg acgaaattgc cacttcgtcg
     4561 gatcccttgc ctctgtgtct gatgctcgta gggttggagg atcgccgaca gtcacttgtg
     4621 tcgcttcagc cttccttggc gagggtgttc gacgtggtgg aactccggac gtggtcagac
     4681 gcggaaaccc gggacttttt tgttaatgcc ttctcgaaag tgggcattca cgtagaagaa
     4741 gaggccctca aaatcttagt gcgttttacg ggggggtttc ccgttatggc gcatgaaatt
     4801 ggggacgcga cctttaatga agacacagac aatcggatta cgcgagagga tgcacttcaa
     4861 ggggttgtcc gagcagcaga catcgtgggg cgcaaacacg tggaaccgca ggttttcacg
     4921 gcgattcgta gcccgcgata tcgagcaatt ctcagaaaga tcgcatcaaa gccattcgag
     4981 gtagaatttc gcaggcgtga cctttggcca cttcttaaag acgacgaaag gaaggtattc
     5041 gacaactttc tcgcccgcat gaagaaactt ggggtcgttc aagcgaaccc agaaggtggt
     5101 cccggtgcat atcgttttac caataccttg cactacctct atttctggtt ggaagccgcg
     5161 cgggcgaagc aatctaaaga attatgaccc gcgcccgcag gtcagtcgaa agagtgtctt
     5221 cttgcaaagt atcactatcg ccgcgcaaaa gaacgttttt caaaggacct atagcgatgt
     5281 tcgccctcag gcaatcgtta ggcaaaggct tctggggcat acagcttgga tgaccgtacc
     5341 ggccatgttt gccggtcaat ccggtcaggt gaaaaggccg tagggggttg ccaatgctct
     5401 gtgcgctacc tggaaacgga agcgcgtttt gccagcattt ctgttggcta aagggacatc
     5461 ttgccgcgat ggtcggaatt ccttaatctg gcgcgaatac gtttgattgc agattaggag
     5521 ctatacgatg tccgcagaag cccgggactc tgttccattt tttcaacgtc gcgaacgact
     5581 tcgccgccga ctcagtaagg acggcctggc gggaatgctt attaccaaaa ccgagaatgt
     5641 ccgttacctc actggctttg gaggtgacga tagctatttg ctcctttgga aagaccacga
     5701 aatccttatc agtgattcgc gcttcacgga gcaaattcgt caggagtgtc ctggtctgga
     5761 ggcccatatc cgtcgcacgg gcgtttcaat gcaccaggcc gccgcaaacc taatcaatcg
     5821 ggcgaaattg acctccgtgg ccgtcgaggc gtccctcccc atggcgactt acaaaaagct
     5881 gaaggcggcc ctcaaacaga cggagcttgt tgaggcagag gattggatcg agcaattgcg
     5941 ggcggttaag gataagacgg aaattgcccg cattcgcaag gcggtccagt acgcagaacg
     6001 ggggtttgca gcggtccggg ccacgctccg cccggaacat tcggagaaag aaatcgccga
     6061 cctattagag tatcaaatgc gcaccttggg agcacgttgt tcttcctttc caaccatcgt
     6121 ggcggtcgga gaaagggccg cgctaccgca tgcaattcct tcggatagga aagttagtga
     6181 aaatgtcttc atcctgatag actggggggc tgacgaaggt tggtacaaga gcgacttgac
     6241 ccgtgtcctt attactggta gaatctcccc caagtttgct aaggtctatc aaacggtttt
     6301 gcgtgctcag caagcggcga ttgctgctgt gcggccgggt gtgtccgccc aggaggtcga
     6361 tgcggcggcc cgccaagtca ttgcagatgc cggtttcggg cggctttttg ggcatggatt
     6421 ggggcacggg gtgggtctgg aaatccatga attgccgcgg cttgggccaa acagctcaat
     6481 taccctcaaa cccggcatgg tgataaccat tgagccaggg atttacatcc ccgattgggg
     6541 tggggtgcgg ctggaggatg acgttcttgt gacgcgggat ggttgtgagg tcctcacccg
     6601 agtccctaag gatttggaag aggtcgtcgt cttttaattg acacaattgc cagtagaaag
     6661 aggtttgact tcgcgcatgg cctcggacgt tcctgacaac gaaggtcgag acttcccaga
     6721 ggtgccagac gtcttcgatc ttgctcgcgt tcgtgagttg gtcgagttga tgcgggcaca
     6781 tgagttaagt gaaatcgatc tgagagaggg caaacgccgg attcgtttgc ggcgcggacc
     6841 tgagagtgtc ccggcagcga ccgggccggc agttcccttt cccatcttca tgcctggcag
     6901 cgcgccagtg cccccgccaa caccggccgg accggttaca cccccgcccg ttcgggagcc
     6961 ggaggcgact tctgaggcgc ccaaacagaa ggaagaagag aacattgtct tcattcgcag
     7021 tcccatggtg gggacgttct atgcagcgcc agatcccgat tcgccccctt atgtcaaagt
     7081 cggtgatatg gttgggccgg atacggtggt cgggatcgtc gaggccatga aggtgttcaa
     7141 tgagatcccg gccgaagttt ccggacagat cgtcgccatc ttggtagaaa acggggaacc
     7201 ggtcgaatac ggtcagccat tgttcaaggt agataccaaa gcctgagtcg gctcgatgtg
     7261 acaaaatagt caaatgggcc acctcatatc ccggttgtag cgcatgttta agaggattct
     7321 tatcgccaat cgaggcgaaa ttgctttgcg agtgatccgg gcctgtcgtg agatgggcat
     7381 cgagaccgtt gtcgttttca gtgaagccga ccgcgggtca gcttatcttg aattggccga
     7441 cgaggcgtat tgcattggtc cccccaaggc atctgagagt tacctccgct acgatcgggt
     7501 gatcagtgcc gcggaaatcg gaaacgccca agcgattcat cccggctacg gttttctctc
     7561 agaaaatgcc cattttgccg aagtttgccg caattgtcag atcgagttta ttggtccccc
     7621 gcatgaggcg atggcgctac ttggggacaa gaacgccgcc cgggcgttgg ccaagaaggt
     7681 aggtgtccca attgttccgg ggagtgaggg cactgtcgag acggaaaccg aggcgattcg
     7741 ggtggcgcga gagattggct atcccgtcct cattaaggca gccgccggtg gcgggggaaa
     7801 agggatgcgg attgccgtta acgagaccgc cttgaagacg gcctttcagc aggcgcaaca
     7861 agaggccgag gctgcgttcg gcaacggcgc tgtctatctg gagaaatata ttgagcatcc
     7921 gcgccacgtc gaggtccagg ccatcggaga ccagtacggc aacgtagtac acttgtggga
     7981 acgcgactgt acgattcagc gtcggcatca gaaattgatc gaggaaagtc cggcgccgca
     8041 gctcccccaa gaggttcgac aagcgatctg tgaggcagcg gtaaaactct taaaggccac
     8101 caattactac aatgcgggaa ccgtggagtt cttggtggat cggcagttca atttctactt
     8161 tattgaggtc aatgctcgaa tccaggtcga gcatcccgtc acggaaatgg tgacgggcct
     8221 agatttggtc aagttgcaaa ttaaagttgc ggccggggaa ccgttaccat tcaaacagtc
     8281 ggatgttgaa catcgtgggg tggccattga gtgtcggatc aatgccgagg acccagaacg
     8341 gcaattccga ccgtcaccgg gtcggatcga gcggatgttt gtccccgggg ggccgggagt
     8401 ccgtttcgat tcccatgtgt atcccggata tgttgtttcg ccccattatg attcgatgat
     8461 tgggaaattg atcgtccatc ggccgacgcg ggcagaggcc atcgccgcga tgaaaaaggc
     8521 cctggaggaa ttgcgaattg aaggaatcaa aacaacgatc ggcctgatcc aggcgattct
     8581 ttcgcatccg acatttgtgg atgcgcgggc cgatacgact tttgtggaac ggacgtggac
     8641 atttaaccaa gcttaagggc tcgtcctttt cggacgttca tgtgagcggg ttctcaaggc
     8701 gggaaaatct cgggatcgcc ttctgcggtt ccggaaccat agggggttgc ggggcgtaat
     8761 aagagtctat cgcaaaaagt cgccctctcc ctctgggaga gggttgggat gagggcaagc
     8821 gaacgtcgga atcgattgag aaaaccggc
//
LOCUS       DAOSUT010000165         8732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4248_length_8732_cov_7.295148, whole genome shotgun sequence.
ACCESSION   DAOSUT010000165 DAOSUT010000000
VERSION     DAOSUT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_8732_cov_7.295148"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2813
                     /locus_tag="PLD05_13030"
     CDS             45..2813
                     /locus_tag="PLD05_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78411.1"
                     /translation="MSRCGNRLFLIVAALGVGICVTLGCPQRSERTKSATTKEPSSSP
                     WSSIEQRQETAPTSSDARPHPLLGDAQEWLVGSGVPDLADKIEQKWLALANTLRSALA
                     KASDLQTLVIDYPPNEAIFPPEIIPPTILWHDSAEPADTWLVEFTVGDGQAWVGALVP
                     GSPPPQGEIDPRCIAETNEIYRGTPYQQSAKSWTVHDNIWQWVKQQSVERPAQLRIVG
                     FSSRRPHVPLSVGQVTLSTSRDPVGAPIFYRDVPLAPSATERGVIKPLSEAFLPLIAW
                     RLRDISQPKSRLLLTNLLTCANCHSFSDDGTTLGMDVDGPSGDKGAYAIVPVARETII
                     DTKDIISWNSFSGKPPGHKTIGFLSRVSPDGRYVVTTLNESVYVQNFKDYRFLQVFYP
                     TRGILGYYDRENGEIKPLPGADDPAFVHCDPVWTPDGQFIVFARAEARDPYPPGYRPA
                     QRANDPAEPQIQYSLYRIPFNGGRGGTPEPIEGASHNGMSNTFPKVSPDGKWIVFVKC
                     RNGQLLRPDSELWIVPATGGTARKMRCNTPLMNSWHSFSPNSRWMVFSSKANTPYTQM
                     FLTHIDEEGNDSPPILISDATAANRAVNLPEFVNRPYEEFLAIRVPAVEYLEIGMRGI
                     RLFEAGKLDEALTQFELAVEKQPDYLEGHVSIAVILIEKGQWKEAVPRLEKALALDPK
                     CWFAHANLGIIRQQEGRIAEALEHFQKAVEIMPNNLNARLNLGRALVDQGRLEEALLH
                     FQAATKMAPTHAASQLNLANVYLELGKLEEAKLAYEAALQSDSRIIEARLGLAETLAQ
                     QQRYQESLAELNKALQLAPDDPMVKATAALFLATCPVDNLRDGRRARQLAEEACRVTG
                     HIDPVCLRSLAVACAENGDFATASDVLEKAITLAERFSPPLAIELRQYRDRFSRREPI
                     RRIDQASQ"
     gene            complement(2894..3358)
                     /locus_tag="PLD05_13035"
     CDS             complement(2894..3358)
                     /locus_tag="PLD05_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78412.1"
                     /translation="MKFTYSLIVLLSFALVAIVGCGKGTDPNRPKTVKAGGVVLYNGQ
                     PVEGAVVTLVPMDNSSPRGAVGQTDAQGRFSLTSFDPNDGAVPGKYYVGIAKVVLEDA
                     PPGQTGPGGEQDPEAKVARDLLPLKYKVPQQSGLTAEITASGPNELKFELTD"
     gene            3602..4738
                     /locus_tag="PLD05_13040"
     CDS             3602..4738
                     /locus_tag="PLD05_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78413.1"
                     /translation="MVRLVKLRAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQ
                     CTNNLKQLALAAHNYADVHKVLPPGETGTGTMWGDNTPPADPVNTNTQRLSTWVLLLP
                     YYEQQALYSQISGPLNIGGTVLPAWGRAPNTNDPGNTGNTRFYPPWTTQIDVLMCPSD
                     GKIRQKGANDQGRTNYRTSVGDSIYRGYARDGSVATCRGLFGLREGCPFASITDGTSN
                     TVMFSERLFGGTPALIKEGYATNVTSFNTCTNNLCIPLECVNVRNPARPNEINTTYGT
                     ANWSGRRWACGIPQYTRFNTVLPPNSPACNDSGWDERNAVITASSNHPGGVNVAMADA
                     SVRFVSETIDAGNPALPEVQVGLSPYGIWGALGSKDGGESVAAP"
     gene            4823..6412
                     /locus_tag="PLD05_13045"
     CDS             4823..6412
                     /locus_tag="PLD05_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP78414.1"
                     /translation="MIKLPTVTLLAGCLFLSCSWIGCRKEVVALPEFDASCVNPSGSD
                     LVRQIELFCGDCHTLPRPEAFPRDRWPAEVRIGYEQYARSGRNDLAPPPIAAVINYYC
                     QQAPEKIAFSLPEDQGGELARHFLSEDVDWNPNENQVPAVSGLLWSSLEPSAQGWLIV
                     SDMREGTVRALAFGQDALAVRLLAHAEFPCRVERCDLDGSGRWGFVIADLGSFFAVDH
                     DRGKVLWLKPSHQEGAYETVTLASRLGRIDDVRVADFDGDGDQDILVAEFGHYHTGGI
                     LFLEQQARSDGQLTFVRHRLDLRTGTIHVPVFDFNGDNRPDFVALISNESESLDLFVN
                     LGKKKFLARPIWRAPDLTFGSSGIELSDLDGDGDQDILYTNGDTFDNLYANPSHGLQW
                     LENIGANGFQYHRLVTLPGAYRALAADLDGDGDQDIVLSSWLSRNVKPANLRTMPIPS
                     VLLLEQINSKEFRVQVLDRDWPQYPVLEVADLDGDGDLDIVAGRHVGLDDPAGVNLPR
                     LRVWWNQKVPGRKPNSDKPSE"
     gene            complement(6414..8318)
                     /locus_tag="PLD05_13050"
     CDS             complement(6414..8318)
                     /locus_tag="PLD05_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOP78415.1"
                     /translation="MLLLIVFAPRVIAWYARQQAEKALHEGRITAAQGWLAWASRCRP
                     ADGRVDILRAGCCRRLKDVEGFVKALQEAEEKKAPPGLIERERRLAAIQAGRLPPGPN
                     NQIGSLITEGYDPAAVAEAFIYGALARGDFAQAWEILSAWSADSPDDGNQNYMAGIYW
                     QFVNQPQKAEEMHRAALRRQPRHELAMLALAEILSTEDRWTELFQLYKVWQRLVPRSK
                     AIRIGLARCARLLGRWDEAWQWLNSDGLSSNLSTARVREIGELLLEMNYPDEAMKWFE
                     RLDIKGTTDRTLLHAVALTESLKGKSLEASQVMDRADRRFQVVTRIHELQMKLTVYPD
                     NHQLRAELENLAKNAPARSPVPDSGSDTTPKSPAQRNSEVRAIYERHCSACHGDQGAG
                     NGRAARFLFPRARNFRRDAFRIVSTEDGIPTVDDVASVIRQGMPGTAMRSFPELSDEE
                     TVQLAELVLEMFREGIREQLVSLYAAEGESVSDEDITEEVLMRTTPGNRVSVPKMGVP
                     DKPILDRGKKAYSLLGCANCHGEDGAGPCDLALWDDQGFPNPPRDLRHGVFKGGDQPE
                     SLYLRLRIGMPGTAHPSCLNTTPEELVSVVHYCRTLIQEPRRTTTNDQRWREAQSRAW
                     YPAYLSPGGP"
BASE COUNT         1835 a         2427 c         2481 g         1989 t
ORIGIN      
        1 cttcatctca tttggcgcat gcttaaacgc gtgcgatgta gaaaatgtca cggtgtggga
       61 acaggctctt cttaattgtc gcggcgttgg gggtcgggat ctgcgtgacc ctcggatgcc
      121 cccagcgttc cgagaggacc aagagcgcga ccactaagga accgtcgtcc tcaccgtggt
      181 cgagcattga acaaaggcaa gagacggccc cgacctccag cgacgctcgt ccccatcctc
      241 tcttgggcga tgctcaagag tggctagttg gttcgggagt tcccgaccta gctgacaaga
      301 tcgagcaaaa gtggttggct ttggctaaca ctctccgcag cgctttggcc aaggcttccg
      361 accttcaaac attggtcatt gactatcccc ctaacgaggc catcttccct ccagagatta
      421 ttccgccaac gatcctctgg cacgattccg ccgagccggc cgacacatgg cttgtggagt
      481 ttacggtggg agatgggcaa gcatgggttg gggccttggt gcccggcagt ccgccaccgc
      541 agggcgagat tgatccgcgg tgcattgccg aaaccaacga gatttaccgt ggcacgccgt
      601 accagcaatc ggcaaagtcc tggacggttc acgataacat ttggcaatgg gttaaacagc
      661 aatcggtgga acgtcccgcg caactgcgga ttgttggctt ttccagtcgt cgtcctcatg
      721 tccctttatc ggttgggcaa gtgacattga gtacatcgcg cgatccagtg ggtgctccga
      781 tcttctaccg ggacgtgccc ttggcacctt ctgccacaga gcggggtgtt atcaaacccc
      841 ttagtgaggc ctttttgccg ctgattgcgt ggcggctgcg agatatttcc caacccaaga
      901 gccgtcttct tcttacgaac cttttaacct gcgccaattg ccattcgttt tccgacgatg
      961 ggacaaccct cggcatggac gtggatggtc cctcgggcga caaaggtgcc tacgccattg
     1021 tgccggtagc gcgggaaacc attatcgaca ccaaggatat cattagctgg aattcgtttt
     1081 cgggaaaacc gcctgggcat aagacgatcg gttttctctc gcgtgtttct cccgacggcc
     1141 gctatgttgt taccactctc aatgaatcgg tctatgtcca aaacttcaag gattatcgtt
     1201 ttctccaggt gttttatccc acgcgaggga ttttgggtta ttacgaccga gagaatggag
     1261 aaatcaaacc actgccgggt gcggacgacc cggcctttgt ccattgtgat ccggtttgga
     1321 cgccggatgg gcagttcatt gtctttgccc gtgccgaggc ccgtgatccc tatccgccgg
     1381 gataccgacc tgcgcaacgg gcgaatgacc cggcggaacc gcaaatccaa tacagcctct
     1441 atcggattcc gtttaacgga ggccgcgggg gcacaccgga acccattgaa ggggcgtcgc
     1501 acaacgggat gagcaacaca ttccccaagg tctcccccga tggaaaatgg atcgtgttcg
     1561 tgaaatgccg caatgggcaa ctccttcggc ctgatagcga gctttggatt gttcctgcca
     1621 ccggcggaac ggcccgcaaa atgcgatgca atacgcctct catgaattcg tggcacagtt
     1681 tttcgccgaa tagccgatgg atggtctttt cctcgaaggc caacacgcct tatacccaaa
     1741 tgtttctcac gcacatcgat gaagagggca acgatagccc acccattctc atttctgacg
     1801 cgactgcggc caatcgggct gtgaatctgc cggagttcgt caatcggccg tacgaggagt
     1861 tcctcgctat tcgtgtccct gcggtggagt acttggaaat cggtatgcga gggattcgtc
     1921 tctttgaagc aggaaaattg gacgaagccc tgacacagtt tgagctagct gtggaaaagc
     1981 agcccgatta cctggaaggt cacgtgagta tcgcggtcat cctgatcgaa aagggccaat
     2041 ggaaggaagc ggtgccgcgt ctggaaaagg ccttggcgct ggatcccaag tgttggttcg
     2101 cgcatgcgaa tttgggcatc attcgccaac aggaggggcg aatcgctgag gccctcgagc
     2161 atttccagaa ggcggttgaa atcatgccga acaatcttaa tgcccgtctc aatctgggcc
     2221 gcgcccttgt ggatcaagga cgactggaag aggccctact tcattttcaa gcggccacca
     2281 agatggcccc gactcatgcc gccagtcagc tcaatctcgc caacgtgtat cttgagcttg
     2341 gcaaactgga agaggccaaa cttgcctacg aggccgctct gcaatcggat tctcgaatca
     2401 ttgaggcccg gctgggactg gccgagacct tggcccaaca acagcggtac caggaatctc
     2461 tcgcagagtt aaacaaagcc ttgcaattgg cccccgatga tcccatggtc aaagcaaccg
     2521 cagcactctt cctggccacc tgtcctgtgg acaatctgcg ggatgggcga cgggcacgcc
     2581 aattggctga ggaggcatgc cgtgtcacgg gtcacatcga tccagtctgt ttgcggagcc
     2641 tagctgttgc gtgtgccgaa aacggcgact tcgctactgc ctccgacgtg ctggaaaaag
     2701 ccatcacgtt agcagaaaga tttagccctc ccttggcgat cgaactacgt caataccggg
     2761 atcgtttttc tcgccgcgag cccatccgtc gcatcgatca agcgagccaa tgaggataaa
     2821 tggtgcccct gcccacagtc gcacgaagga gcaccacatc ccggcgaaaa gcagacgatt
     2881 ccgccagcat tcttcaatcg gttaactcaa atttcaactc gttcgggccg ctggctgtaa
     2941 tctctgctgt cagtccagac tgctgcggaa ccttatattt gagtggcaga agatcgcgag
     3001 ccactttggc ttcagggtct tgctcgcctc caggtcccgt ctgaccgggt ggagcgtcct
     3061 ctaaaaccac tttggcgata cccacataat acttgcctgg tacggcccca tcattggggt
     3121 cgaacgacgt cagcgagaac cgcccctggg cgtctgtttg ccccacggca ccacggggac
     3181 tgctgttgtc catgggaact aacgtgacaa cggcaccttc aaccggttgg ccattgtaca
     3241 acacgacgcc gccggccttg accgtcttgg ggcgattggg atcggtgcct ttcccacacc
     3301 cgacaatggc aacgagcgca aatgaaagca acacgatcag actgtaagtg aacttcatgg
     3361 ctaatacctc ctttgaaccg accggcctga cgagctttcg cacactgtcg gattgtattg
     3421 tcaccatggg aaattgcaac cccacccata attagcgcat taacccacct gttaatgggt
     3481 attaattatc gcgaatggga tctgcaaaaa tgttgcgtgc taacagtttg gagtgtaaac
     3541 tacagcgttg ttccggtgca actttttcgc tttgggtttc tcgtttgtat ggaggtgacc
     3601 tatggtcaga cttgtcaagt tgagagcgtt tacgcttgtg gagctacttg tggtgattgc
     3661 cattatcggc attctgattg cgctgctttt gccggcagtt caggcagcga gagaggccgc
     3721 tcggcggtcg cagtgcacga acaatctaaa acagctggcc ttggccgcgc acaattatgc
     3781 ggatgtccac aaggtcctgc cgcctggtga aaccggaaca ggaacgatgt ggggtgataa
     3841 taccccgcca gccgatcctg taaacacgaa cacgcagcgg ctttcgactt gggtcttgct
     3901 attaccctac tatgaacagc aggcccttta ttcgcagatc agtgggccgc tcaatatagg
     3961 tggtactgtt ctaccagctt ggggccgtgc gccgaatacg aatgatcctg ggaatacggg
     4021 gaatacgcgt ttctatccac catggacgac gcagattgat gtgttgatgt gcccctcgga
     4081 cggtaagatt cgtcagaagg gtgcgaatga ccaggggcga acgaattacc ggacgtcggt
     4141 gggcgattcg atctaccgtg gttatgcacg tgacggttcc gtggcgacgt gtcgagggct
     4201 ttttgggcta agggagggct gtccctttgc cagcattacg gacggcacaa gcaacaccgt
     4261 catgttctca gagcgattgt ttgggggaac gccggcactg atcaaggaag gatatgccac
     4321 aaatgtgacg agctttaata cgtgcacgaa caacctgtgt attcccctgg agtgtgtcaa
     4381 cgttcgtaac ccggctcgcc ccaacgagat caacaccacg tatgggacgg ctaactggtc
     4441 agggcgtcgc tgggcatgtg ggattcctca gtacacacga ttcaacacag tgttgccacc
     4501 gaacagtccg gcttgcaatg acagtggctg ggatgaacgg aatgccgtca ttacggcctc
     4561 cagcaaccac ccagggggtg tcaacgtggc catggctgat gcctcggtcc gtttcgtttc
     4621 ggaaacgatt gatgcgggaa atccggcctt gcctgaggtc caagtgggtt tgagccccta
     4681 cggcatctgg ggcgccttgg gaagcaagga cggtggtgag tcagtcgccg ctccttgatg
     4741 cggcgcggac cgtccaccat aattgcctca catgatggca atgaaggtac acaactggcc
     4801 atgggggtca cggaccgtgg gtatgatcaa gttaccgacc gtgaccctct tggccggttg
     4861 cttgtttttg tcgtgttcgt ggatcggctg tcgcaaagag gtcgtggcgt taccagaatt
     4921 cgacgcttcc tgcgttaacc cctccggatc agatttggtt cgccaaatcg agcttttctg
     4981 tggggattgt cacacattgc cacggcccga agcctttccg cgcgaccgct ggcctgcgga
     5041 ggttagaatc ggctacgagc aatatgcccg ttctggccga aatgatttgg cgcctccgcc
     5101 aatcgctgcg gtcattaact attactgtca gcaggcaccg gaaaaaatcg cgttttcgtt
     5161 acccgaggat cagggtggtg agcttgctcg ccattttctt tccgaggatg ttgactggaa
     5221 tccgaatgaa aatcaggttc ccgcggtgtc gggcctgctc tggtccagtc tggaaccgtc
     5281 tgctcagggc tggctcatcg tgagtgatat gcgcgaaggc acggtgaggg ccttggcctt
     5341 tgggcaagac gccttggcgg tccgtcttct tgcgcacgcc gagtttcctt gccgtgtcga
     5401 acgatgcgac ctcgatggga gtggccgttg gggctttgtc attgcggacc tgggaagttt
     5461 ttttgctgtc gatcatgatc gaggcaaggt cctttggtta aaaccgagcc accaggaggg
     5521 ggcctacgaa accgtcactt tagcgagtcg gcttggtcga attgacgacg ttcgtgtggc
     5581 cgacttcgat ggggatggtg atcaagacat ccttgttgca gagttcggac attaccacac
     5641 aggtgggatt ctctttctgg aacaacaagc tcgttcggac ggacagctaa cgttcgttcg
     5701 tcatcggctt gatcttcgca cgggaacgat ccatgttccg gtgttcgact tcaacggcga
     5761 caatcgaccc gactttgtgg ccctgatcag caacgagtcg gaatcgctcg acctttttgt
     5821 gaatcttgga aaaaagaagt ttctggctcg accgatctgg cgtgcgccgg acttgacgtt
     5881 tggctcaagc ggcatcgagc taagcgacct ggatggagac ggggatcaag acatccttta
     5941 cacaaatggc gataccttcg acaaccttta cgctaatcct tcccatgggc ttcagtggtt
     6001 agagaatata ggagcgaatg gttttcaata ccatcggttg gtaacgctgc cgggggcgta
     6061 tcgggccttg gcggctgatc tcgacggcga tggagaccag gatattgtcc tatcgtcgtg
     6121 gttgtcacgg aacgtcaagc cagcgaatct gcgaacgatg cccatcccct cggttctcct
     6181 tttggaacag atcaactcca aggagttccg tgtgcaggtc ctcgacaggg attggccgca
     6241 gtatcctgtg ctcgaagtgg ctgacctaga cggcgatggc gacctggaca ttgtggccgg
     6301 tcgtcacgtc ggtttggatg atccagccgg tgtgaattta ccgcggttac gcgtgtggtg
     6361 gaatcagaag gtacctggac gaaaaccaaa cagcgacaag ccatccgagt gagttacggt
     6421 cccccagggc tcaagtatgc gggataccag gctctgcttt gagcctctcg ccatcgttgg
     6481 tcgttggtcg tcgtgcgacg tggttcctga attagggtcc gacagtagtg gaccacgctg
     6541 acaagctcct cgggcgtcgt attaagacat gaaggatggg cagtaccagg catcccgatg
     6601 cgaagtctca agtagagaga ctcgggttga tcgccgccct taaacacgcc gtgtcttaag
     6661 tcccgcggcg gattgggaaa cccctggtca tcccaaaggg cgaggtcaca tggcccggcg
     6721 ccatcttccc catggcaatt ggcgcagccg aggagcgaat acgccttttt cccgcgatcc
     6781 aggatcggct tatcaggaac gcccatttta ggaaccgaaa ctcgattgcc cggcgtcgtt
     6841 cgcatgagaa cctcttcggt gatgtcctcg tccgagacgc tttcaccttc tgcggcgtaa
     6901 aggctcacaa gttgctcccg aatgccttcg cgaaacattt ccagtaccag ctcagctagc
     6961 tgcaccgtct cttcgtccga aagctccgga aacgatcgca tggcggtgcc gggcattccc
     7021 tgacggatga cactcgccac gtcgtcaacg gtggggattc cgtcttccgt actcacgatg
     7081 cggaaggcgt cacgacgaaa attgcgggcc cgtgggaaca ggaaccgtgc ggctcggcca
     7141 ttgccagctc cctggtcacc atggcaagcg gaacaatgac gctcatagat cgcgcggacc
     7201 tcggaattgc gctgcgcggg cgatttcggt gtcgtgtcgc ttccggaatc ggggacgggc
     7261 gatcgtgcgg gagcattctt tgcaaggttc tccagttccg ctctgagctg gtgattgtcg
     7321 ggataaaccg tcaatttcat ctgcaactcg tgaatgcgtg tgaccacctg aaaacgccgg
     7381 tcggcacggt ccatcacctg actagcctcg aggctctttc ccttcagtga ctccgtcaaa
     7441 gcgacagcgt gaagcaacgt cctgtccgtt gtccctttga tatccagtcg ctcgaaccat
     7501 ttcatcgctt catccgggta attcatttcc agcagaagtt cgccgatctc tcggacccgg
     7561 gcggttgaca aatttgacga cagtccatcg ctgttaagcc attgccaggc ttcgtcccac
     7621 cgccccaaga gccgggcaca tcgtgccagt cctattctga tcgctttact tcggggaacc
     7681 agacgctgcc acactttgta cagttggaag agctctgtcc agcgatcctc ggtacttagg
     7741 atctcggcca gggcaagcat ggccaactcg tggcgcggct gcctgcgtaa agcagcccgg
     7801 tgcatctcct ccgctttctg aggttgattg acaaattgcc aatagatgcc cgccatgtaa
     7861 ttctgattgc cgtcatcggg cgagtcggcg gaccaggctg acaggatttc ccaggcttgg
     7921 gcgaaatcac cgcgtgccaa ggcgccgtag ataaaagcct cggccaccgc cgcagggtca
     7981 tatccctcgg taatcaacga gccaatttga ttgttcggtc ccgggggaag ccgtcctgcc
     8041 tggatggccg ccaaccgccg ttcccgctcg attagccctg gcggagcctt tttctcttcg
     8101 gcctcctgga gggctttgac aaatccctcg acatccttga ggcggcgaca acatcctgcc
     8161 cgcaaaatat cgacccgtcc atccgcggga cgacagcgcg aggcccaggc cagccagccc
     8221 tgggctgccg tgatccggcc ttcgtggagg gctttttcgg cctgctggcg tgcgtaccat
     8281 gctatgactc tcggtgcgaa aactatgagt aagagcaatg ctatcagacc gacacccaaa
     8341 gcaaaaaggc ggctttttgg gccaggcttt ttccgggcgg attgagttgt cgttgatcgg
     8401 cgtgaagaca tgttccgtta aaatgcacgt gcttgtcgat accgcgacgt atcccgagca
     8461 accattggtg gtagcggcat tgcggccacc agcgtgggcg tcggcccagt gcgcgtgatg
     8521 gaaaaaataa caaaggtctt tgaccaccag ctcggttcac tcgggccaca ttggcgtcag
     8581 tgtagcgtca ggccagacgg cgtacaacgc atttactcgc gatcacagac ggtcgatggt
     8641 tgacaaggac gagggcgaac cgcatcatcg aaagatcgac gtattctgtt agccctgccg
     8701 cggcatgttg atccagagaa gttttagttc gc
//
LOCUS       DAOSUT010000166         8706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4257_length_8706_cov_10.436250, whole genome shotgun sequence.
ACCESSION   DAOSUT010000166 DAOSUT010000000
VERSION     DAOSUT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_8706_cov_10.436250"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLD05_13055"
     CDS             <1..677
                     /locus_tag="PLD05_13055"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOP78416.1"
                     /translation="LLTAAGELFAERGFEGATARQICERAGVNLAAINYHFKSKEQLY
                     IEAVKAAAPVFHAELAELEVELDQIQEERRRDAAAALLKKYIYLLMHRMVGIHVPDWK
                     VKLIMREMLEPTGGCQEFFRQHVQRGFEILMRIVNLLLPEDTPEHRRVQIGLSIVSQC
                     LHYRMARDVIQFLIPRRDDPAFSPDSLAEHIVEFVFSALGLSPPFVRQWERKDCEYRE
                     GAHLSG"
     gene            797..2215
                     /locus_tag="PLD05_13060"
     CDS             797..2215
                     /locus_tag="PLD05_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOP78417.1"
                     /translation="MSYRAVAERSWFEVIFNVVAPFVVIGLGILGFLTLKALKKPVPR
                     EKPQSPLPLVQTTRVVRHEGGLELKTDGVVVPYREIQIATEVDGRIVEKTADCEAGRY
                     VTKGTLLLTIDKSDYQIEYARLQEQLRQAEASLAELDVEINNTQRLLEVAEQQFEIQK
                     QDFARAERLFGTKAISQAEFDAARKSQLLARQELINLENLLRLKQSSKERLVAARNQV
                     EVSLKKAELDLSRTEIRAPSDGVVISDLVEQNSYVRKGTVVVTFEDTSVAQVRCNLTT
                     RQVAWLWALIHGKTLENADRGEAYQVPKLPVTVEYNLDGRVFVWNGVLDGYEGIGVDD
                     RTRMVPCRVLVDRPTVVSIKAGEGKSKESLVDVPRHPPALVRGMYVTVRIYVAPGVPV
                     LRVPERAVQPGGMVGRIRDGKLKMIPVELAEVVPEGALIVAPPDILQEGDSVAVSPTP
                     FIENNPLAISGDGLPVEEQPVL"
     gene            2212..3270
                     /locus_tag="PLD05_13065"
     CDS             2212..3270
                     /locus_tag="PLD05_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOP78418.1"
                     /translation="MKSILRWSIRNAPAMNTLLIGLLVVGTVSLLRMRREVFPDFNLD
                     MILISVPYPGASPTEVEEGICQKIEEAVRAVAGIKKTYSVARESGGAVILELFSNADA
                     QRVLDEVRSEVDRIPSFPEMAENPEIKLITIRRAAITVGVIGPDSDDPRSERALRELA
                     EQVRDELIALPNVNQADLMGVRPYEISVEISEKTLREYGLTLQKVAQVIRQENIEVPG
                     GTLRAHSQEVLLRGKNKGMTGKELASIPLVTQPDGRILTLGDLATIQDGFADTTAVSR
                     INGRPGMAVVVTSTSTEDLLKTTKDVHEYVAQKKLPPGYELVTRGIFRFMSAIGSIFW
                     PRMVCRGLSSCSCSSPYF"
     gene            3210..5726
                     /locus_tag="PLD05_13070"
     CDS             3210..5726
                     /locus_tag="PLD05_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOP78419.1"
                     /translation="MAKNGLQGLIIVFVLLAVFLEIHLAFWVALGIPVAMLGTAIIMQ
                     WSDQTLNMMSVFAFLMALGMLVDDAIVIGENVYVHRQMGKGYVQAAIDGTYEVLPSVT
                     AAVATTVIAFAPLFFVPGIMGKFIWLLPFGVVTMLLVSLMEAVFILPCHLAHDPSKWE
                     RQFGGTAEENSFAGSDAPQPVRSGRRWLGIIWRVSLTFLLVGVLLILSRGEIPWEMSW
                     PVWVICVIIVALITFARALPRIEGFTNLINRYADIALQTFIKRVYMPSLKLALRFAPV
                     TLALAIALLIASLGLVVSGYVPFVPFPKLDAESIEAVVYYPAGTPEDVTNRATLAIEQ
                     AIRDVDEEYRRQGRPLVKTIYRVVGEVTVGALRVGNELPTGSHSGKVEVELVEAAVRD
                     LSSEEIVKRWRERSLELGRQFPGAESVTFQSQARGPGGLPIEFRLLAPVHRMAELEEA
                     VEKCKAYLAEIPGVFDIQDDARPGKWEFQIKTTDKAKALGVSLLDLAGTVRAAYYGEE
                     AMRLQRGRHEVKVMVRYPEEERRSLARFDDIRIRAGGIERPITELAEITVARGYSQIH
                     RLNQLRAIAITADVDEAVVNSQKVITDLKTRFLPDLLSNPRYSDIQISWEGAQESTNE
                     SISAMIRGLLVALIAMFALLTLEFRSYFQPIMILIIIPFGLLGAIVGHLIMGLPLTLM
                     SIFGMVALTGVVVNDSIVLVDFINSRVREGKPIREALIESGQRRLRPVLLTSVTTIGG
                     LTPLVLERSVQAEYLVPMAVTLSFGLMFTTVIALILVPTVYLMYSWVMPVSRKDEEEI
                     EESWKKEHPPAEKEESYVDTIPKVPEGALPEPFLMESPQD"
     gene            5982..7004
                     /locus_tag="PLD05_13075"
     CDS             5982..7004
                     /locus_tag="PLD05_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HOP78420.1"
                     /translation="MNLREERYSRFALVREMMETPEIIAQFDPAVVADAVPIVQEVKN
                     LFLTGEGSSRIFPAKNLIYENLKRGGVWHIATDGARQAHEYDLKQFAVFAASNSGKTK
                     EVISLFTKLAQEGHPRRLGLTANYGVKLEEVATRCYHLKCGSENAVAATKSVVEQALF
                     YRAMLACQENDSSFWRLKEKAAAAAREVLEMPIPQSLVDRIAQAPLVYFAGRNNGVAE
                     ELTLKTNEITRKKSDFLEGTYAVHGIEEVMDANEVMIVVEPFAAEAEKFHQTLVERVG
                     LTIVAISAEETMFPTIRIPHVPGYETILQLLAGWNLLVEVGVTMGIDLDKPVRARKIG
                     NEYLPG"
     gene            7155..7979
                     /locus_tag="PLD05_13080"
     CDS             7155..7979
                     /locus_tag="PLD05_13080"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOP78421.1"
                     /translation="MQSGVKYMRRLDDRVMASEEAADVFDQWEVGQERWMFVSPHDDD
                     AVIGAGLTIQVGMAAGAEVYVVITTDGRMGYCRIPQRRTITRIRSEEAREAYRMLGLP
                     PERLRCLGFPDCNLNAYRGRHFTTIGDPTEIEGASGLQNAFPYVLRQIRPTRVFLPTP
                     TDLHPDHRIVYEEMLISLFHAQGSIWPELGPPIVRIPVVYEFATYCDFPEPPQIRLVT
                     PVAFLERKLEAIQCFASQEQIEVLVKVQRDVGPVEYLRQVAFNFYSPEKYAALFEG"
     gene            8489..>8706
                     /locus_tag="PLD05_13085"
     CDS             8489..>8706
                     /locus_tag="PLD05_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78422.1"
                     /translation="MMKRRLTMALLGWSWMCWIGLVSAQVYPTAAFDKGVGGCAACGD
                     VGCGGCTSCGGWLSDFLYCDPCRPKDPW"
BASE COUNT         1904 a         2116 c         2600 g         2086 t
ORIGIN      
        1 ggcttttgac agctgccggg gaactatttg ccgagcgagg gtttgagggg gccaccgccc
       61 gccaaatttg cgaaagggcg ggcgtcaatc tcgccgcgat caattaccac ttcaagtcga
      121 aggaacagct ttacattgag gccgtcaagg cggccgcacc ggtgtttcac gcggagctgg
      181 ctgagctaga agtcgaactc gatcagatcc aggaagaaag gcggcgggac gcggcggcag
      241 cgctcctcaa aaaatacatc tatctcttaa tgcaccgtat ggtcgggatt cacgtccctg
      301 actggaaggt caagctgatt atgcgggaaa tgctggagcc gacagggggg tgtcaggagt
      361 tctttcggca gcatgtccag cgagggtttg agatcttaat gagaattgtg aacctccttt
      421 tgccagagga cacgccggag catcgccggg ttcagatcgg tttgagcatc gtttcgcagt
      481 gccttcatta tcgcatggca cgcgatgtga tccagttttt gattccgcgt cgagatgatc
      541 cggcgtttag cccggacagt ttggcagagc atatcgtgga gtttgtcttc tccgccctag
      601 gattgtcgcc gcctttcgtt cggcaatggg aacgtaagga ttgcgaatat agagaaggtg
      661 cccatctgtc cggatgatgg acgcggtcaa tcgccagtgt gagagaccga aaacccaatt
      721 actggacatg gagataaaca tcattcggtg aagaccgaaa ggtttctgcc gaggaataag
      781 cggaaagggt ggttgcatgt cttatcgggc cgttgcggaa cgatcttggt tcgaggtcat
      841 tttcaatgtc gtggctccat ttgttgtcat tggcctgggg attctcggct tcttaaccct
      901 gaaggccttg aagaagcccg tgccccgtga aaaacctcag tcgccgttgc cgctcgttca
      961 gacgacacgg gttgttcgac atgagggtgg cctcgagcta aaaaccgacg gcgtcgtcgt
     1021 tccctaccgc gaaattcaga tcgcgacgga agtggatggg cgaattgtgg aaaaaaccgc
     1081 tgactgcgaa gcagggcgat acgtgaccaa gggcacattg ttgttaacga tcgacaaaag
     1141 tgattatcag attgaatatg ccaggcttca ggaacagttg agacaggcag aggccagttt
     1201 ggctgagctg gatgttgaaa ttaataacac gcagcggctc ctggaagtgg cggagcaaca
     1261 atttgagatt caaaagcagg attttgcccg cgcagaacgg ttgtttggca caaaggcgat
     1321 cagtcaggcg gagtttgatg cagcgcgcaa gagccagctt ctcgccaggc aggaattgat
     1381 caatctcgag aacctcctgc gccttaaaca gagtagcaag gagcgtttag tcgctgcgcg
     1441 gaatcaggtt gaggtatctt tgaaaaaggc ggagttggat ctttcccgga cggagatccg
     1501 cgcacccagc gacggtgtcg tgatcagtga tcttgtcgaa caaaatagct acgtgcgaaa
     1561 gggcacagtg gtggtgacct ttgaagacac gtctgtggcc caggtgagat gtaatctaac
     1621 gactcggcaa gtcgcatggc tttgggcttt gatccacggg aagacgttgg agaacgcgga
     1681 ccggggggaa gcttatcagg ttccaaaatt gccggtaacg gttgagtaca acctcgatgg
     1741 gcgtgtgttc gtttggaatg gtgtgctaga tggatatgaa ggaatcggcg tggatgatcg
     1801 cacccgcatg gttccttgcc gagttcttgt agatcgcccc accgttgtct caataaaagc
     1861 cggcgaaggg aaatccaaag agtccctagt tgatgttccg cgacatcccc cggcccttgt
     1921 tcgcggaatg tatgtcacgg taaggattta cgtcgctccc ggagtgccgg tgcttcgcgt
     1981 tcccgaacgt gctgtccaac ctggggggat ggtgggacgg attcgtgacg gcaagttgaa
     2041 gatgatcccg gtggaacttg cggaagttgt tcctgagggg gcattgatcg ttgccccacc
     2101 cgatatctta caagaaggcg atagtgtggc ggtttcgccg acgccgttta ttgaaaacaa
     2161 tcccttggcg atttcaggag atgggctgcc agttgaggag cagccggtct tatgaagagc
     2221 attctccgtt ggtccattcg caacgccccg gccatgaaca cgctgctcat tgggctgctt
     2281 gtggtcggga cggtgagtct tctccgaatg cggcgagagg tcttccccga ttttaacctc
     2341 gacatgatcc tgattagtgt cccctatcca ggggcaagcc ccacggaggt agaagaggga
     2401 atttgccaga aaatcgagga agccgttcgg gcggtggcgg ggatcaagaa gacctattcc
     2461 gtggcaaggg aaagtggtgg tgccgtcatc ttggagctat tcagcaatgc cgatgcccag
     2521 cgcgtcttgg atgaagtccg ttcggaggta gatcgcattc cgagttttcc ggaaatggcc
     2581 gagaatccgg agatcaaact tatcaccatc cgccgtgcag cgattaccgt gggggtgatt
     2641 ggaccggact ccgacgatcc tcggtcagaa cgggcccttc gtgagttggc cgaacaagtg
     2701 cgggatgagc tgatcgcatt gccgaatgtt aatcaggctg acctgatggg cgttcgcccg
     2761 tacgaaattt cagtggaaat ctctgaaaag acgttacgcg agtacggctt gacgctgcaa
     2821 aaggttgccc aagtgatccg tcaggagaac atcgaagtgc cgggtggaac gctccgggcg
     2881 cactcacagg aagtgcttct ccggggcaag aacaaaggca tgacgggcaa ggagcttgca
     2941 agcattcctc tcgtcacaca accggacgga cgtattctaa cattgggtga tttggcaaca
     3001 attcaagatg gttttgccga caccacggca gtgagccgga tcaacggacg cccgggaatg
     3061 gctgtcgtcg ttacgagcac ctcgacagag gacctcctca agacgacgaa agatgtccac
     3121 gaatatgtag cgcagaaaaa gttgcccccg ggttatgaac ttgttaccag ggggatattt
     3181 cggtttatgt ccgcgatcgg ctcaatcttt tggccaagaa tggtttgcag gggcttatca
     3241 tcgtgttcgt gctcctcgcc gtatttctag aaatccattt ggcattttgg gtggcacttg
     3301 gcatccctgt cgccatgctg gggacagcca tcatcatgca gtggagcgat cagaccctga
     3361 atatgatgtc cgtgttcgcg ttcctcatgg ccctggggat gctggtcgac gacgccatcg
     3421 tcatcggtga aaacgtttat gtccaccgac agatgggcaa gggctatgtg caggcagcaa
     3481 ttgacggaac atacgaagtc ctgccttccg tgactgctgc ggttgcgaca acagtgatcg
     3541 cctttgcccc gttgtttttc gtcccgggca ttatgggtaa gtttatttgg ctcctaccgt
     3601 ttggggtcgt gacgatgctt cttgtatcgt tgatggaagc cgtgtttata ttgccttgcc
     3661 accttgccca tgaccctagc aagtgggaac ggcagtttgg aggtacggcg gaggaaaact
     3721 ccttcgctgg atcagatgcc ccgcaaccgg tgcgctcagg ccgacgttgg ctgggaatca
     3781 tttggcgagt cagtctcaca tttctcttgg taggggtatt gttgattttg agccgtggtg
     3841 aaattccttg ggagatgagt tggcccgttt gggtcatttg tgtcattatt gtggccttga
     3901 ttactttcgc ccgtgccctg ccgagaattg aaggctttac gaaccttatc aatcgttacg
     3961 cggatattgc gttgcagact ttcatcaagc gtgtttacat gcccagtctt aagttggctt
     4021 tgcgttttgc gccggtgaca ctggctctgg caattgcact cttgatagct tcactgggcc
     4081 ttgtagtctc cggatatgtt ccatttgttc ccttcccgaa gctcgatgcc gaatccattg
     4141 aggccgtggt ctattatccg gccggtacac cggaggatgt cacgaatcgg gcgacgctcg
     4201 ccatcgaaca ggcgattcgt gatgtggatg aagaataccg ccgtcagggc aggccgcttg
     4261 tcaaaacaat ttaccgagtg gtgggagaag tgacggtggg cgctttgcgg gtgggcaacg
     4321 agctgcccac gggcagtcac tcgggcaagg tcgaggtgga acttgtggag gcggcggtgc
     4381 gggacttaag cagcgaagag attgtcaaac ggtggcgcga acggtcgtta gaattgggcc
     4441 gtcaattccc gggtgctgaa agcgtgacat ttcagtccca agcacgcggg cctggtggat
     4501 tgcccatcga gtttcggctt ttggcaccgg tccatcgaat ggcggaactt gaggaggcgg
     4561 tcgaaaaatg caaagcgtat ctggctgaga ttccgggtgt cttcgatatc caagatgatg
     4621 cacggcctgg caaatgggaa tttcagataa agacgaccga caaggctaag gccttggggg
     4681 tttcgctcct cgatttggcg ggaacggtcc gcgcggcata ttacggcgaa gaggcgatgc
     4741 gacttcaacg gggccgtcat gaggttaaag tcatggttcg ctatccggaa gaggaacgtc
     4801 gctccctagc tcgctttgat gatatccgaa ttagggcagg ggggatcgaa cgccccatca
     4861 ccgagctggc ggaaatcacc gttgccagag gctattcgca gattcatcgt ctcaatcaac
     4921 tgcgggccat agctattacg gccgacgtgg atgaagccgt tgtcaattcg caaaaagtca
     4981 ttaccgattt gaaaacccgg ttccttcccg acctcttgtc caatccccgc tattcggata
     5041 tccagatctc ctgggaaggt gcccaagagt cgaccaacga gtcgatttcc gccatgatcc
     5101 gcgggctttt ggtggcccta atcgctatgt ttgcactctt gacactcgaa ttccgctcgt
     5161 attttcagcc tatcatgatt ctcatcatca ttccgtttgg gctgctaggt gcgatcgtcg
     5221 gacacctcat aatgggtctt ccgctgaccc tcatgagcat ttttggaatg gtggccctca
     5281 caggtgtggt ggtcaatgac tccattgtgc tggtggactt tatcaattcg cgggtccgtg
     5341 agggtaaacc gattagggag gccctcatcg aatcagggca gcggcgctta cgtcccgtcc
     5401 tgttgacctc ggtgaccacc atcggtgggc tgactccgct tgtcctggaa cggtcggttc
     5461 aggctgaata cttggtgccg atggccgtaa cgttgtcgtt tggcctcatg tttaccacgg
     5521 tgattgcctt gattctggtc ccgacggtgt accttatgta cagttgggtc atgcccgtat
     5581 cgagaaaaga cgaggaagaa atcgaggagt cttggaaaaa ggagcaccct cctgcagaaa
     5641 aggaggagtc ttacgtagat actatcccca aagtccccga aggtgccttg ccggaacctt
     5701 ttctcatgga atctcctcag gattgaaccg tcgcggctgc ggccgtttgg gtggccgccc
     5761 cctttgtctt cagttagact cgacggacga cttgctccca cgggtgggtc tcgcaggtcg
     5821 ggatcgacgt cttataatgg gaacatgaat cgttaaggca gtgtcggaga gttttgaagg
     5881 agcgaggata gatttcgttc gaactcggcg gtcttgttgg tcttgcatct aagggaagcg
     5941 ggtgccaaaa gagccccttt tatacgccta ggagtttccc aatgaatctg cgcgaagaac
     6001 gttatagccg ctttgctctg gttcgggaga tgatggaaac cccggaaatc atcgctcagt
     6061 ttgatcctgc ggtcgtggcc gatgccgtgc caattgtgca ggaggtcaag aacctctttt
     6121 tgacgggcga gggttccagc cggatctttc ccgcgaagaa cctgatttac gaaaacctga
     6181 agcgtggcgg tgtttggcac attgcaacgg atggtgcccg ccaggcacat gagtatgacc
     6241 taaaacagtt tgccgttttt gctgcaagta atagcggtaa gacaaaagaa gtcatctccc
     6301 tattcacgaa gctcgctcaa gaagggcatc cccgtcggct tggtttgacc gccaactacg
     6361 gcgtgaaact ggaggaagtc gcaacgcggt gttaccacct gaagtgtggc tcggaaaatg
     6421 ccgtggcggc gaccaagagc gtggtcgagc aagcactgtt ttatcgggcg atgctggcct
     6481 gccaagaaaa cgattcttct ttctggcggt taaaagagaa ggctgccgcg gcggcacgcg
     6541 aggttctgga aatgccaata ccgcagtcgc ttgttgatcg cattgcccag gctccattgg
     6601 tctattttgc tggccgcaat aacggcgtcg ccgaggaatt gacactcaag actaatgaga
     6661 tcacgcgaaa gaagagtgat ttcctcgagg gcacttatgc cgtccatgga attgaagaag
     6721 tcatggatgc caacgaggtc atgattgtgg tggagccatt cgcggcggag gccgagaagt
     6781 ttcatcagac cctcgtcgag cgagttggcc ttacgatcgt ggcgatttcg gcagaagaga
     6841 ctatgttccc gacgatccgg ataccgcatg taccgggcta cgagacgatc ctgcaacttt
     6901 tagcaggttg gaaccttctc gtcgaagtgg gcgtgaccat gggcattgac ctcgataagc
     6961 ctgtgcgagc tcggaagatc ggaaacgagt acttgcctgg atgatcgccc tcagatgcgg
     7021 cgggtttttt aagtcccgtt tgagcagcct tccaaggaga ggtaacctgg gactgagaac
     7081 ttggagcaat aggagacgcg taatcattta gccattggct tgggtctcaa gtatcaaatc
     7141 cagagggaac tcgcatgcaa tctggtgtga aatacatgcg tcggctcgat gatcgagtga
     7201 tggcgtcaga agaggctgcc gacgtttttg accagtggga agtcgggcag gaacgctgga
     7261 tgtttgtctc acctcacgac gatgatgcgg tgatcggagc aggcctgacg atccaagtag
     7321 ggatggctgc cggagctgag gtctatgttg tgattaccac agacggccgg atgggctatt
     7381 gccgcattcc gcagcgtcga acaatcacgc ggattcgcag tgaggaagcg cgagaggcct
     7441 accggatgtt agggctgcct cctgagcggt tacgatgcct cggatttccc gattgcaacc
     7501 tcaacgccta tcggggacgg catttcacaa cgattggtga tccaactgaa attgaaggtg
     7561 ccagtgggct gcaaaacgcc tttccctatg tgttgaggca gattaggccg acgcgagtgt
     7621 tcttgcccac ccctacggac ctccatcccg accaccgcat tgtctatgag gaaatgctca
     7681 tcagtttgtt tcacgcccaa gggagtatct ggccggagtt gggtccaccc atcgtaagaa
     7741 ttcccgtggt atatgagttc gctacgtatt gcgatttccc tgagcctccc cagattcgcc
     7801 tggtgacgcc agtcgcgttt ctcgagcgta agctggaggc catccagtgt tttgcgagtc
     7861 aagaacagat agaggtgttg gtaaaggttc agcgggacgt tggaccggtg gaatacctgc
     7921 gccaagttgc tttcaatttc tattccccgg aaaaatacgc agctttattt gagggctgaa
     7981 agtgggtagt gcaacgaaga gaagaggttt gggtaatata atctgccagt gacgggattt
     8041 tacaggaccc tctattaaga gcctatccga acaagtcccc ctctccctcc gggagagggt
     8101 tggggtgagg gcaagcgaaa ataggaatcg atggagaaaa ccggcatcga ttcgagccac
     8161 cctcaccccc gacccctctc ccgccgaacg ggcgagggga gattatggga tgggttctaa
     8221 gcgacgattc cccttcacag ggatctgatc tactctttcc cgactttccg gttggctagc
     8281 ggggtgattt tgatcagatt gtaacatctt ctctggttgt tccatttttc caattcagct
     8341 cggtcatttc ggtacattgg ggggctttcg ataaacgtgg ctctcgcgat tagccgataa
     8401 tccatttgag tgatggttct gaggagcgta ttggaaatct cgccgggacg aaccgatcaa
     8461 tttcttcctg ctttttggag agtcagggat gatgaaacga cgactaacaa tggctttgct
     8521 gggctggtcc tggatgtgtt ggattgggct tgtctctgcc caggtttatc caaccgcagc
     8581 ctttgataaa ggggtcggag gatgcgccgc ctgtggcgac gtgggatgtg gtggctgcac
     8641 ctcgtgtggt ggctggcttt ccgacttcct ctactgtgat ccctgtcggc cgaaggaccc
     8701 ctggaa
//
LOCUS       DAOSUT010000167         8606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4303_length_8606_cov_9.591978, whole genome shotgun sequence.
ACCESSION   DAOSUT010000167 DAOSUT010000000
VERSION     DAOSUT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_8606_cov_9.591978"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            960..3911
                     /locus_tag="PLD05_13090"
     CDS             960..3911
                     /locus_tag="PLD05_13090"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOP78423.1"
                     /translation="MGKGTPPAVIVVSETASWLERLAALEVRRYLYLRTGQLLPIQHG
                     LPNGNADVILLARSDRLLESDLLPKETLELYRDSLRERIQRAGKHGYLLWTLPSQGRH
                     VLLLVSSQDVGLLYAAYRLAEHYGVRFYLEGDVIPDMRLAWSLAQIDEVRRPLFEHRG
                     IQPFHDFPEGPDWWSLDDYKAILAQLPKLGMNFFGLHCYPEGGVGPEPLVWIGQAEDI
                     GDGAAVRFSYPARHFLTSNVTGAWGYRPGKTNDYAFGADQLFETDDFGADYMQGYSPW
                     TEMSLEQSNQLFEAMGKVLNEAFTFARQLGIVTTIGTETPLTIPKAVRERLQASGKDW
                     KDPQVVKAIYAGMFKRIMQTHPLDYYWLWTPEDWTWSGTKQEQVDATLTDLALAYEAA
                     REVNAPFTLATCGWVLGPSQDRALFDKVLPKEMPVSCINRQVGHDPVEPGFAAVQGRP
                     KWAIPWLEDDPALISPQLWVGRMRKDAADALAYGCTGLMGIHWRTRQLGPNVAALAAA
                     AWEQQGWNPAWGKAPLPAPPPPEGPQGGRFAAFPGVDFADTDEDLVYQTVRYDVDAYH
                     FDLPNGQYRVILKLCEPHYDAPGKRIFGIKVQGKTVIDTIDIFAKVGKNRAIDFTVDD
                     VPVTEGRLTIEFVRQVEYPCIAGIVVTGPVTRKINCGGPAWGDYQADWPPTEKIANRF
                     LMCTDFYLDWASAQFGPEAAAEIAEVFSEIDGHLPRPSNWVHGPGGIVPDARPWSQVE
                     KEYDFVARLEGLRDRIVGPGNQERFDYWLNLFRYMRANARVNCIYHAYKTAVAEVEKT
                     TEPAERSRLAKEKALPLRQELVAALAEVQKYLLSHVSTYGGLGNVCNWQQHIIPMVLH
                     DTDAELGKLVGEELLRDSDLPKTYTGPSRIILPTVRGLLTRGESLRLKVILLGFENPE
                     ANLLWRPLGEGDYRRVPLSHVARGVYQTQIPGEQLPEIVEYFVEVQDKTRIMRYPATA
                     PTFGLTTVVAQ"
     gene            4227..4448
                     /locus_tag="PLD05_13095"
     CDS             4227..4448
                     /locus_tag="PLD05_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOP78424.1"
                     /translation="MTMDKSLRIRKALVKVRSVLTRAERIKRLQQLEQFGEDRSPLGL
                     PKVRVPKISMKKKKKKAKEEAASGQAAKK"
     gene            4535..5809
                     /gene="larA"
                     /locus_tag="PLD05_13100"
     CDS             4535..5809
                     /gene="larA"
                     /locus_tag="PLD05_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOP78425.1"
                     /translation="MRVTLEYGRTGLNVELPDANVVKVLRYRPAEVLPDPRATIRELL
                     WRPIGTPPLPEIAVGRRSACIAICDITRPVPNEVILSELLPLLEESGIPRERITILIA
                     TGLHRPNLGDELVEIIGKSIAENYRVENHNGQALSEHEFLGESPRGVPVWIDRRYIQA
                     DLKITIGLIEPHFMAGYSGGRKLICPGLAALETVRAWHSPKFLEHPNARAGVLEGNPV
                     HEENTWIGRRAGCDFIVNVVLDEHRKIVHAVAGDMEEAFLDGVRFVEKVVWDTVPEPV
                     DVVITTAAGYPLDATFYQSIKGMVAAEPIVKPGGTVILAAEISEGIGSPEFRRIFEEN
                     SSLDEFLDRITKGDYFRMDQWQLEEMAKVRRKARIVVVTGGLTSDQLSRLFVESAPTV
                     EDAVADCLRRYGPQATIAVMPKGPYLVADVAR"
     gene            complement(5903..8110)
                     /gene="fusA"
                     /locus_tag="PLD05_13105"
     CDS             complement(5903..8110)
                     /gene="fusA"
                     /locus_tag="PLD05_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOP78426.1"
                     /translation="MLITLWDDQKGYRGKRIKRTIVTGETIEKRITHMPNAEPLKNIR
                     NIGISAHIDSGKTTLSERILYYTGRIHRIGEVKGDGPGATMDFMELERERGITITSAA
                     TTVRWGEAVINLIDTPGHVDFTIEVERSLRVLDGAVLVLCAVGGVQAQSLTIDRQMRR
                     YHVPRVAFINKMDRVGADPFRVVQQMKERLDGEPILMQLPIGREADFRGVIDLVSMEA
                     VFFDGQFGEHVRREPIPADLRADAEKARQQMLESLAMYSDELLEVLLAEEKPSEELIW
                     RVAKLATCSLQMTPVFLGSAFKNKGVQPLLDAIVRILPSPLEVEVTAIPPGEDQSPLL
                     LTADPEAPAVAMAFKLMEDPYGALTFLRVYQGTLRKGSSYYNQRTGKRERFGRIVRMH
                     ADKREDIEAATAGDIVAVLGIDAISGDTYCESYPMCHLERMHVPEPVIKMSITPVERG
                     SAERLAKALARFRREDPTLQVTIDEESGETLIAGMGELHLDIYVERLRREYGVDVLVS
                     APKVNYREAPTQPASFNVRHRKQTGGAGQYAHIVGRMEPLPEDCEETFVFEECIFGGH
                     IPKQFIPAIEKGLRSSLEKGPLAGYPIVRLKVIVEDGSYHEVDSSDLAFQICAQQVMR
                     EAFPQTKPALLEPIMKIEIECPQTAQGNVVGDLISRRGVVLGTTSLGNLVRIEGEVPL
                     AETFGYSTTLRSLTQGQGTFTLEFACYRQVPPSIQEQIVFQRKRNRMPAGASV"
BASE COUNT         1878 a         2485 c         2431 g         1812 t
ORIGIN      
        1 tgtgaccact gcacgcctga acgttgctgc tgtgaagggg atggcccctg cacctgctcc
       61 tgcgagtgct gccagacggc cgcgtgctgc caagacagct gtcaggtagg caacggatgc
      121 gccaaggagt gcacctgccc cggttgtcag tgcgctgaag gcggcccctg cacatgcccc
      181 gattgcaagt gcgccgaggg cggtccctgc acatgtgagg gatgtccctg tggggcaccg
      241 tcctctcaac cggtggcttg ctgcggcaag accgctgcgt gattgagcat caccgcaagt
      301 agggacgaac acggatcagt cttgcggtgt aaatcgttga agcgtgcgac gagtacccta
      361 aaggtgtcgt tgcgaacgtc aacaggcctt cgaacggcgt cagccgttcg aaggtttttt
      421 attgtgcaac gtgtaggcaa agagtctatc ccaaaaagtc cccctctccc tccgggagag
      481 ggttgggtga gggcaagcaa tcgtgggagt cgatcgagaa aaccggcagc gattcgagcg
      541 acccccactc tcgacccctc tcccgctgaa cgcatgaagg gagataggct ctaagggcca
      601 agtgcccgag gggcaaacat acgccatgtg ccttttttga caccagagca cgctggcggt
      661 tccgagttgg agatgacccg cgctcatgta ccagcgaatt ggtcttcgac aacttcgaag
      721 gccaccccag tccatctctt gcttgggacc cctaatccct ccaaaataga gccaccaaaa
      781 cattcccaaa aacgcaacaa gggaggaata tgatgtccgc gacgtcggtc gccaacgggg
      841 tcattgtggc aattttcgaa agccaaaaac cgcccaggat attttccggc tggaggggat
      901 tactcttgat cggcctttca tgttgccttt tcgggcgggg tctcgccgcg gagccgtcaa
      961 tggggaaagg cactccgccc gcagtcattg tcgttagcga aaccgcaagc tggctggaaa
     1021 gactggctgc cttggaggtt cggcgctacc tctatcttcg cacaggccag ttgctcccta
     1081 tccaacatgg cctgcccaat ggtaatgccg atgtgattct gctcgcacgg agtgatcgtc
     1141 tgctggaatc cgatctgctc ccaaaggaga cgcttgagct ctatcgagat agcctccggg
     1201 aacgaattca acgagccgga aaacatgggt atcttctttg gacactgccc tcgcagggcc
     1261 gacatgtcct gcttctggtg agctcccagg atgttggcct tctttatgcc gcctatcgcc
     1321 tggccgagca ctacggcgtg cgattttatt tggagggtga tgttattccc gacatgcggc
     1381 ttgcatggtc attggcccaa attgacgaag ttcgtcggcc actttttgag caccgcggga
     1441 ttcagccctt tcatgacttc ccagaaggcc ccgactggtg gagcctcgac gactacaaag
     1501 cgatcctcgc acaattgccc aaattgggga tgaatttctt cggacttcac tgctacccgg
     1561 aaggtggtgt ggggcctgag ccgttggtct ggatcggtca ggcggaagac attggcgatg
     1621 gggccgctgt acgattcagc tatcctgctc ggcattttct cacaagtaac gtcacggggg
     1681 cctggggtta tcgccctgga aagaccaacg actatgcctt tggcgccgat caactttttg
     1741 aaacggatga ctttggggcc gactatatgc aaggttattc cccctggacg gagatgagcc
     1801 ttgaacaatc gaaccaattg ttcgaggcga tgggaaaggt gctcaacgag gcattcacct
     1861 ttgcccggca attggggatt gtaacgacca ttgggacaga aacgccgctg accattccca
     1921 aggcggtccg cgaacgcctg caggcatccg gcaaggactg gaaagacccc caggtggtga
     1981 aggccatcta tgccgggatg ttcaagcgga tcatgcagac ccatccgctc gattattatt
     2041 ggctttggac cccggaagac tggacctggt cgggcacgaa gcaggagcag gtggatgcca
     2101 ccctcaccga tttggccctg gcctacgagg ctgcccgcga agtcaacgca ccgttcacat
     2161 tggccacatg tggttgggtc ctaggaccga gccaggatcg ggcacttttc gataaggttc
     2221 ttcccaagga aatgcccgtc agttgcatca atcgccaagt tggtcacgat cctgttgagc
     2281 caggtttcgc cgctgtgcaa gggcgtccca aatgggccat cccttggttg gaagacgacc
     2341 cagccctcat cagtccccaa ctatgggtgg gacggatgcg aaaggatgcg gcagacgccc
     2401 tcgcctacgg ctgcacggga ttgatgggca tccactggcg gacgcgacaa ttggggccca
     2461 atgtagctgc cctggcagcc gcggcgtggg aacaacaagg gtggaatccc gcttggggaa
     2521 aggcacccct gcccgccccg ccaccaccgg aaggccctca agggggacga ttcgccgcgt
     2581 ttcccggcgt cgatttcgct gacaccgacg aggatctggt gtatcaaacc gtccgttatg
     2641 atgtcgatgc gtatcacttc gatctgccca acggtcaata tcgggtcata ctgaagctct
     2701 gcgaaccaca ctacgatgca ccgggaaagc gtatttttgg cataaaagtc caaggaaaga
     2761 cggtgatcga cacgatcgat atcttcgcca aggtgggaaa aaatcgggcc attgacttca
     2821 ccgttgacga tgtccctgtg accgagggac ggcttacgat cgagttcgtt cggcaggtgg
     2881 aatacccctg catcgccggg atcgtggtca cgggtccggt gacgcggaag atcaattgcg
     2941 gcggccccgc ctggggagac tatcaagccg attggccacc cacggaaaag atcgccaatc
     3001 gtttcctcat gtgcacagat ttctatctgg attgggcctc ggcccagttt ggaccggaag
     3061 ccgcagcgga aatcgccgag gtcttctctg aaatcgacgg ccacctgcct cggccgagca
     3121 actgggtcca cggacctgga gggattgttc ccgatgcacg cccatggtct caagtcgaaa
     3181 aagaatacga cttcgtggca agactggaag gcctacgaga tcgaattgtg ggaccgggga
     3241 accaggagcg gttcgactat tggctgaatc tgttccgcta catgcgggca aacgcgcggg
     3301 tcaattgcat ctatcacgct tacaagaccg ccgtggctga agtggaaaag accacagagc
     3361 ccgctgaacg gagccgtctt gccaaggaaa aggccctgcc tttacgccag gagttggtag
     3421 ccgccctcgc agaggtacag aagtatctcc tgtcccatgt ctcgacctat ggcggactgg
     3481 ggaatgtgtg caactggcag cagcacatca tccccatggt attgcacgac acggatgccg
     3541 agcttggcaa attggtcggc gaagaattgc tccgtgatag cgacctcccc aaaacctaca
     3601 cgggcccaag caggatcatc ctccctacag tccggggtct gcttacacgc ggggagtcac
     3661 ttcggctaaa agtcatccta ttggggtttg agaatcctga ggccaacctg ttgtggcgtc
     3721 ccctggggga gggcgattac cgcagggtac cgctctctca cgtagcacga ggcgtttatc
     3781 aaacgcaaat tcctggcgag caactccctg agattgtcga gtactttgtg gaggtgcagg
     3841 acaaaacccg gattatgcgt tatcccgcca cggcgccgac atttggactc accactgtgg
     3901 tggcacagta ggcacccttt ctaaccattt cattcgcacg atgcatcaaa cccacagagc
     3961 aattttttga caggatgcct cttgggaaca cccacacgcc gattttaaca gaacgtacag
     4021 tcacaaatat tcggccttcg ttgccgtcgg cggatgaaca ttataaaatt gaagttttgg
     4081 caatttcgct cacctgacgc cataagcgcc agttcgctag tagtttaaaa tcccaatatc
     4141 accgagcgcc aattggtcac tcgctattgc cgtactggcg ccaacggaca atagcctgga
     4201 aatacccaaa taaggagtac gaacccatga caatggacaa aagtctccgc attcgaaagg
     4261 ccttggtcaa ggtgcggagc gttttgaccc gtgccgaaag gattaagcgt cttcagcagc
     4321 ttgaacagtt cggcgaggat cgctcccccc ttggcctgcc aaaggtccgc gtgccaaaaa
     4381 tctccatgaa gaagaaaaag aagaaggcta aggaagaggc ggctagcggt caggcggcaa
     4441 agaagtaacg ggtcgccgtt ttcggcctcc cgggtcaggt ccttcatcgg ctgcggggcg
     4501 attttaacgc gtcaccagcg cgaggtgttt gtctatgcgc gtgactttgg agtatggccg
     4561 gacgggtctg aacgtcgaat tgcccgacgc aaacgtcgtc aaggtcctcc gctatcgacc
     4621 cgcagaagtg ctgcctgacc cacgtgctac catccgggag cttttgtggc gaccaatcgg
     4681 aactccgccg ttgccggaaa tcgccgtggg gcgacgttct gcctgcattg ccatttgtga
     4741 catcacgcgc cctgttccca acgaagtgat cctctccgag ctactgcctt tattggagga
     4801 gtctggcata ccccgtgaga ggatcacgat tctgattgcg acggggttgc accgaccgaa
     4861 tctcggggac gaactggtcg agatcatcgg caagtcgatt gctgagaact accgggtaga
     4921 aaatcacaat ggccaggcgc tctctgaaca tgagtttctt ggggaaagtc cccgcggcgt
     4981 ccctgtttgg atcgatcgcc ggtacattca ggcagatttg aagatcacaa tcggactgat
     5041 tgagccgcac tttatggcgg gctactccgg tgggcgtaag ttgatttgtc ccggattggc
     5101 cgccttggaa accgttcgcg cctggcattc tcccaaattt ttggagcacc ctaacgcccg
     5161 ggcaggcgtt ctcgaaggaa accccgtcca cgaggaaaac acgtggattg gccgtcgcgc
     5221 cgggtgtgat ttcatcgtta acgtggtcct ggatgagcat cgaaaaattg tccatgccgt
     5281 tgccggcgac atggaagagg cctttctgga cggtgttcgc tttgtagaaa aggtcgtctg
     5341 ggataccgtc ccggaaccgg tggacgtcgt catcacaacg gcggcaggct atcccctgga
     5401 cgccacgttt taccagtcga tcaaggggat ggtggcggcg gagccaatcg tcaagccggg
     5461 cggcaccgtt atccttgcgg cggaaatttc ggaaggaatc ggtagcccag aattccgccg
     5521 tatctttgag gaaaacagtt ccctcgacga gttcctcgac cgcattacca agggtgatta
     5581 cttccgcatg gaccagtggc agttggaaga gatggccaag gtgcggcgga aggcccgcat
     5641 cgtggtcgtg accggtgggc tgacatcaga tcaactttcc cgactctttg tggaaagtgc
     5701 cccaacggtt gaagacgcgg tcgcagattg cctccggcgt tatggaccac aagctaccat
     5761 cgcagtcatg ccaaagggac cttatcttgt ggcggacgtc gcccgctgat tttattgctg
     5821 ccaaagaagc cggttgaaat caccgtggcg aacgttccac ccagcggaac catctcggcg
     5881 ctcgagaatc ttcgagaatc tttcagaccg aagcccctgc cggcattcgg ttccgtttcc
     5941 gctggaagac gatctgctct tgaatggacg gcggtacctg ccggtaacag gcaaactcca
     6001 gcgtgaatgt cccttggccc tgggtaaggc ttcttaaggt ggtcgagtag ccgaacgtct
     6061 cggccagagg cacctcgcct tcaatccgca ccaggtttcc cagtgacgtc gttccgagga
     6121 caacgccgcg cctgctgatc aagtcaccaa cgacgttgcc ctgtgccgtc tgcgggcatt
     6181 cgatctcaat tttcatgatc ggctccaaaa gggccggttt ggtctgcggg aaggcctctc
     6241 gcatcacttg ctgggcacaa atctgaaacg ccagatcaga gctatcaacc tcatggtatg
     6301 agccatcttc tacgatgacc ttaaggcgaa cgatggggta accggccaga ggtccttttt
     6361 ccagggaact tcgcagcccc ttttcaatgg cagggatgaa ttgcttgggg atgtgtcccc
     6421 caaaaatgca ttcctcgaag acgaatgtct cttcgcagtc ttcgggaagc ggttccatgc
     6481 gaccgacaat atgcgcatac tggccagcac cgcctgtttg ctttcggtgt cggacgttga
     6541 aactggccgg ttgcgtgggg gcctcacggt aattcacctt cggggcactg acgagcacgt
     6601 caacgccgta ttcgcggcga aggcgctcca cgtaaatatc caggtgcaac tcgcccatgc
     6661 cggcaatgag cgtctcaccg ctttcctcgt cgatggtcac ctggagcgtc ggatcttcgc
     6721 gccgaaagcg ggccaaagcc ttggccaggc gttccgccga gccgcgttcc accggcgtga
     6781 tggacatctt gatgacgggt tcggggacgt gcatccgctc caagtggcac atcggatacg
     6841 actcgcaata ggtatcaccg cttatcgcat cgatgcccag aactgccaca atgtcgcctg
     6901 cggtagccgc ctcgatgtcc tcccgcttgt cggcatgcat gcggacgatt cgaccgaacc
     6961 gctcgcgttt accagttcgc tggttgtaat aagaactccc cttgcgaagc gttccctgat
     7021 agacccgcag gaaggtcaaa gctccataag ggtcctccat caatttgaag gccatggcaa
     7081 cggccggggc ctccgggtcg gctgttagca acaaaggcga ttggtcctcg ccgggaggaa
     7141 tggccgtgac ttccacctcc agcggcgacg gcagaatcct cacaatagcg tcgagcaaag
     7201 gttggacacc tttgttcttg aaagccgaac ccaggaacac cggcgtcatc tggagggaac
     7261 acgttgctag cttggccaca cgccagataa gctcctcgga aggcttttcc tcggccagaa
     7321 ggacttccaa tagttcgtca ctatacatgg ccagcgattc gagcatctgc tgcctggcct
     7381 tttctgcgtc tgcgcggaga tcggccggta tcggctctcg gcggacgtgc tcaccaaact
     7441 gaccgtcaaa aaagacggcc tccatactca ccaaatcgat gacgccgcgg aaatcggcct
     7501 cccgaccgat ggggagctgc atcaggatgg gttccccatc caatcgttcc ttcatttgct
     7561 ggacgacccg aaagggatcc gccccgacgc ggtccatctt gttgataaag gccacccgcg
     7621 ggacatgata ccgccgcatc tggcgatcga tggtcagtga ttgggcctgc actccaccta
     7681 cggcgcagag gacaagcacc gccccatcga ggacccgtaa actccgttca acctcaatgg
     7741 tgaaatcgac gtgcccaggg gtatcaatca aattgatgac agcctctccc cagcggacgg
     7801 tggtggcggc actcgtgatt gtgatccccc gttcccgttc caactccatg aaatccatgg
     7861 tagcgccggg gccatcacct ttcacttccc caatgcgatg aatccgtccg gtgtaataga
     7921 gaatccgctc gctcaacgtc gtctttcccg agtcgatatg agcggaaatc ccaatgttgc
     7981 ggatgttctt caaaggttcg gcgttcggca tgtgtgtgat acgtttttcg atagtctcac
     8041 ccgttacgat tgttcttttg atacgcttac cccgataccc cttttggtcg tcccacaagg
     8101 tgataagcac cggaaacaag catcggcttc tggcgggcgg gatcgctgca tgcgctgtga
     8161 agcgcccctg agggactacg tagcaggccg attacaccgg cccatagcgt aacacctcga
     8221 caggcaccct catgaacaca cggaatggtc tcccccgcca cccagctacc atccaccatt
     8281 ctagccgacc tggtccctcc cccggcaata gctgcccaca gacctttcga actctctcac
     8341 ctagctgtgc tgaaggaaca gccccggcat agaagggata aatagggtgg gaattttcaa
     8401 gtagatcaat tataaaccct ttccgcgcgt ttgtccacca tgcgaggatt gaaaccccgt
     8461 aggggcaggc ccccgtgcct gccccaaaat gcaattagcg ttaatcacgc ccggctctgc
     8521 gcgaaatcga aacgaccgga tgcccgacca aacggaaacc gctcgccgtg cgcagaaacg
     8581 gggcaaccac agggggttgc ccctac
//
LOCUS       DAOSUT010000168         8558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4323_length_8558_cov_9.846407, whole genome shotgun sequence.
ACCESSION   DAOSUT010000168 DAOSUT010000000
VERSION     DAOSUT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4323_length_8558_cov_9.846407"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLD05_13110"
     CDS             <1..657
                     /locus_tag="PLD05_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78427.1"
                     /translation="AKVVTEQVPVEVVSYQPQEVRRKVPVQVCRMVEEEIVRKVPVTV
                     MRPVVERVEKKVPVQVCRMVTEEQVRQIPVTTYKWVTEERVEQVPVQVCRTVAEEHTI
                     TIPRTVQKYVPVTYTCYRPRTVVCRVAVDPCLVPITSCPAPVSTSVTPAPSTESATPT
                     PAQAGAPSPSDQPPALPRETQRPAEEKPLPGPAPMEEKSAPQSPSTPAQPPAPSGSTT
                     "
     gene            complement(680..1342)
                     /locus_tag="PLD05_13115"
     CDS             complement(680..1342)
                     /locus_tag="PLD05_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78428.1"
                     /translation="MSKLNIPIPVRSLFHFAIPCYYQQPLWGDEGALLSEAHRLPDLS
                     QVEGEFNGVEVRAGWNEEAFAAQFRVFNPKLLVWTAKGTGSVFISVWINTRAAQDIHR
                     ATRFCHWFEIEGCKGRTGVEEARVTWRAIPRAKETPNSPPPQTVIAKAKLDPDRRHYC
                     VNVLFKRESLTGYDPREYPQIGFNYEVMRAAVEPITFSAGAPLRYQDDPSLWITLDLV
                     KP"
     gene            complement(1393..8541)
                     /gene="uvrA"
                     /locus_tag="PLD05_13120"
     CDS             complement(1393..8541)
                     /gene="uvrA"
                     /locus_tag="PLD05_13120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOP78429.1"
                     /translation="MAASDIVVQGAREHNLRNVNIVLPRNHLICLTGVSGSGKSSLAF
                     DTLYAEGQRRYIESLSTFARQFIGQMPKPNVDRIEGLSPSICISQKTAGSNPRSTVGT
                     ITEIYDFLRILYARAGTAYCPQCGERIQAQPRQLIVEQILESFTGKTVLVLAPLIRGQ
                     KGEYQSLLATLRRRGFARARIDGEVVRLDQDLRLRRHMRHDIELVVDRLEVSPAQRSR
                     LAEAVESAIGLSDGEVMVVCEGNEATDGSQQRGTARGLVRRFSSRFACPNCQISFAQP
                     TPQLFSFNSPEGMCQRCQGLGQVETVDPRKFIENPHRSIQQGVISALGRWKEMLPDVR
                     YLLRRWASILEEELGYEKGALLETAWEELPEQIQEILLFGENARNVPWAVKEKLVWNS
                     RITGGVAGILEVLPETLVQASAREKLQEARRVSVCPSCQGSRLRPEARFIRLQTSHPR
                     FEDNPSRSLPEVCALPVADTRLFFTDLVLDQIQQKIAEEPLKEIRNRLDFLLNVGLDY
                     LTLDRSAPSLSGGEMQRIRLASQIGSGLVGVTYVLDEPSIGLHPRDNDRLLATLAKLR
                     DQGNTVVVVEHDEDTIRAADYIVDFGPGPGSRGGQIVAAGSFEDIVKSPDSLTGQYLT
                     GKRCIPVPTKRRQPTDKKLIIRGARHNNLKNIDVEIPLGLFVCVTGVSGSGKSSLVSD
                     ILVEALHRDLNGGVGQPGAFDRIEGLEHLDKMIAIDQSPIGRTPRSNPATYTKLFDDI
                     RKLFASLPEAKVRGYKPGRFSFNVKGGRCEACEGHGATKLEMDFLADIWVTCPVCNGQ
                     RFNRLTLQVLYKGKSIADVLEMEVREALELFENIPPIRHKLETLHAVGLDYMKLGQPS
                     PTLSGGEAQRVKLARELVKRSTGRTIYILDEPTTGLHFADIEMLLKVLHSFVEAGNTV
                     VVVEHNLEVIKTADWIIDLGPEGGEAGGWVVAAGTPEDLAKVEASHTGRFLRMALAGE
                     TLQGNGEEKKRKAFFESLKTQRQQSKPITSVSVWGARQHNLKGINVEIPRDKLTVCCG
                     RSGSGKTSLAMDTIYAEGQRRYVESLSTYARQFVSQMQKPKVDRVEGLSPAIAIEQKH
                     AANTPRSTVGTVTEIYDYFRVLMARLGQPYCPECNVPVGTQTVDQIVSKVLDHPEGTR
                     IYILAPLEIQDETDFGEIWNQLRAAGYSRIRVNGVIHSLEDPPRLDRRRRHQVEVVVD
                     RLVVRPDQRSRIAGSVENALTLGKGVLAVLYPRDRVPEPLWKVVTHSQHFACHRCGRS
                     FKPLSANNFSFNSPLGWCPGCQGLGVEMGADPAALILDPKKGLLDQALVIWPRTNKPL
                     ARAMVEAFCRDTGIPGDVPFGELPSHARRLIFHGTGERWFDVYVEDYARIAQGLDGTK
                     SSQQSPEDASSPQLLLRFQYRGLYPCLEAAASSVPSLRTVLSSFITEVECTACAGSRL
                     RDDASAVRFRGRTIDEWCRLPLGRLIAEIRMWQLDERERQIAGEVVREIENRVQFLVD
                     VGLDYLTLARSAPTLSGGESQRIRLAAQIGSGLCGVLYVLDEPTIGLHPRDNRRLLDA
                     LRKLRDLGNTLLVVEHDREVINSADHLLDFGPAAGEDGGQIVAEGHPSTIVHDTRSVT
                     GPYLSGQKAIPVPKNRRMPSWWNEKVAEAAILEDASRQDSPGKKIWRSLTPPGKGWLE
                     VHGARHNNLKNINVAIPLGTFTVVTGVSGSGKSSLVEDILYPALARELHRSQATPGAH
                     DGLGGLEMINKVIRVDQQPLGQTPMSTPATYTGAFDAIRDLFASLPEARRRGFTRRRF
                     SFNVPGGRCERCEGAGQLKIEMHFLPDVWVECDACKGRRFDAETLSVTYHGKSIADVL
                     EMSCEEALALFSNVPSIARILTILCDVGLGYLRLGQPANTLSGGEAQRVKLAAELARP
                     DTGRTIYLLDEPTTGLHFDDLKKLLDVLNRLVDLGNTVVVIEHNLDVIKSADWVIDLG
                     PEAGDYGGYVVAVGTPEDIVAYSRWWQARQTKTNGRKRDKRRATLPPTAETPEPAFPW
                     RCYTGEVLAPVLEAGPHVEIKRFIPPGKTQPTGQPTLPLEFATPETSTQEVKMPWEVN
                     GREWHLTRECAVNGKPIRWNRQILELVMTTIDEIRRFSEPDWNSQNVVEVRPYKKSLG
                     WFLHAYTGDDSQLRLVFRTSARAAKAASPSIFKLTHATQPRKMNSWLSDFPEPSEKLP
                     SPVLERRVVRPWHEVEIRVTELTQLDRKKFCDLIGDLAEGFFELAESSDRGKAAYDVM
                     WHVSSEGFSGEPRWNPRILLRLTQVIRQCDSNLKLMYHQRHAVIVGDDVTKHVWAKIL
                     TKDPEALRVIVRAPRDAFTVGELVKIGGTWTLDQTRSDEARIKLSIVSLDELNVGSLA
                     NFMKTALTVFREFVSKTV"
BASE COUNT         1835 a         2566 c         2356 g         1801 t
ORIGIN      
        1 gcgaaggttg tgaccgaaca ggtgcccgtt gaagttgtga gttatcagcc gcaggaagtg
       61 cgtcgcaagg tccccgttca agtctgccgg atggtggaag aagaaatcgt gcgaaaggtg
      121 ccggtcacag tgatgcggcc ggtggtggag cgtgtggaaa agaaggtccc cgtccaagtt
      181 tgccgcatgg tcacggaaga gcaggtccgg cagattcccg tcacgacgta caaatgggtt
      241 accgaagagc gagtcgagca agtgccggtg caagtctgcc gcacggtggc tgaagaacac
      301 acgataacga ttcctcgaac ggtacagaag tatgtgcccg tcacgtacac ttgttaccgc
      361 ccgcggacgg ttgtctgccg tgttgcggtg gatccctgct tggtgccgat cacttcctgc
      421 ccggccccgg tgagcacctc tgtcactccg gcaccttcga cggaaagcgc gacaccgacc
      481 ccagcccagg cgggagcacc aagcccgtcc gaccagccac ctgccttgcc gcgcgaaacg
      541 caacgaccgg cggaggagaa gccgcttccc ggtccggccc ccatggagga aaagtccgct
      601 ccgcaaagcc ccagcacacc ggctcagccg cctgcccctt cggggagcac gacttgacca
      661 accggcggct tgtcgccact cagggtttga ccaggtccag tgtgatccaa agactggggt
      721 catcctgata gcgaagcgga gcccctgcgg aaaaggtgat aggctctacc gcggcgcgca
      781 tcacttcgta gttgaagccg atttgcggat actcgcgggg atcatacccg gtcagtgact
      841 ccctcttaaa caggacattg acgcaatagt gccgtcgatc cgggtcgagt ttcgcttttg
      901 caatcacagt ttggggtggg ggtgaattcg gtgtttcctt ggccctgggg atggctcgcc
      961 aggtgacccg cgcttcttcg acgcccgtgc gacctttgca cccctcaatt tcgaaccagt
     1021 gacaaaaccg cgtggcgcga tggatatcct gagccgcccg cgtgttgatc cagaccgaga
     1081 tgaacacgga cccggtgccc ttggcagtcc agactaagag tttcggattg aacacgcgga
     1141 actgggcagc gaaggcctct tcattccaac ccgcccgcac ttccacacca ttgaactctc
     1201 cctccacctg gctaaggtcc gggagcctgt gtgcctcgga aagcaaagcc ccttcgtcgc
     1261 cccataaggg ctgctgataa tagcacggga tcgcaaagtg gaataggcta cgaacaggga
     1321 ttgggatatt taactttgac atgctagctg ttgtccgcaa ttggggtctc aacacgggat
     1381 cattgtgttg tctcataccg tcttcgagac gaactcccga aagactgtga gagcagtctt
     1441 cataaaattc gccaacgacc cgacattcag ttcatccaga cttacgatac tcagcttaat
     1501 gcgggcctca tcggatcggg tttggtcgag tgtccaagtg ccgcctatct tgaccagttc
     1561 ccccactgtg aaggcgtccc gcggggcacg cacgatgacg cgaagggctt caggatcctt
     1621 cgtcaaaatc ttggcccaca cgtgtttggt gacgtcgtct ccgactatta cagcatgacg
     1681 ctggtgatac ataagcttga ggttggaatc gcactggcga atcacttgcg tcagtcgcaa
     1741 taggatgcgg ggattccacc gaggctcccc ggaaaaccct tccgaagaga cgtgccacat
     1801 tacgtcgtat gcggcttttc ccctatccga ggactcggca agctcgaaaa agccctcggc
     1861 caagtctcca atgaggtcgc aaaacttctt tctatcgagc tgtgtcagtt ccgtaacacg
     1921 gatttcgacc tcatgccaag gccggacgac acgcctttcc aaaactggcg acggaagctt
     1981 ctccgaaggt tcaggaaagt cgctgagcca tgagttcatc ttccggggct gcgttgcatg
     2041 cgtcagcttg aaaatgctcg ggctggcggc tttggcagcg cgggcggagg tgcgaaagac
     2101 caatcgcaat tggctgtcat cgccggtgta ggcatgaaga aaccagccca gcgatttctt
     2161 gtatgggcgg acttcaacga cattctgcga gttccaatca ggttcggaaa accgccggat
     2221 ctcatcaatc gtggtcatca caagttcgag tatctgccta ttccagcgaa taggcttgcc
     2281 gttcaccgca cattcgcgtg tgagatgcca ttctcggccg ttgacctccc agggcatctt
     2341 gacctcttgg gtagaggtct ccggcgtggc aaactccaag ggaagtgttg gctgccctgt
     2401 gggctgcgtt ttgccgggcg gaataaaacg cttgatctcg acatgagggc cagcttcgag
     2461 aacgggtgcc aggacctcgc ctgtgtaaca acgccaaggg aaagccggtt ccggcgtttc
     2521 ggccgtcggc ggaagtgtgg ccctccgttt gtcacgtttg cgaccattgg tcttagtttg
     2581 ccgggcttgc caccagcggg aataggccac aatgtcttcc ggcgtaccaa ctgccaccac
     2641 atatccgccg taatcaccgg cttccggtcc aaggtcaatg acccagtccg ccgacttgat
     2701 gacatccagg ttgtgttcga tgaccaccac ggtgtttccc aaatccacga ggcgattaag
     2761 gacatcgagg agctttttca aatcgtcgaa gtgcaaaccc gtggttggct catcgagcaa
     2821 gtagatggtt cgcccggtgt caggtcgggc cagttccgcc gccaatttca cccgctgggc
     2881 ttcgccgccc gataaggtgt tggcaggttg ccccaggcga aggtaaccga gtcccacatc
     2941 gcacaagatg gtcaaaatgc gggcaattga ggggacattg ctaaagaggg ctagggcttc
     3001 ctcgcatgac atctccagga cgtcggcgat cgatttgcca tgataggtaa cagagagggt
     3061 ttcggcatca aaccggcgtc ctttgcaggc gtcgcactcg acccaaacat cgggcaagaa
     3121 gtgcatctcg atcttgagct ggcccgcacc ttcacaccgt tcgcatcgcc ccccggggac
     3181 attgaagcta aaacggcgac gggtaaaccc acggcgtcgg gcttccggca aggatgcgaa
     3241 cagatcgcgg atggcatcaa aggcacctgt gtatgtggcg ggggttgaca tgggcgtttg
     3301 accgagcggc tgttgatcga cgcggatcac cttgttgatc atttctaagc cgccaagtcc
     3361 atcgtgggcg cccggcgtcg cctggcttcg gtggagttcc cgtgccaagg ccggatacag
     3421 gatgtcctcc accagggaac tcttgccact cccgctgacg cccgtaacaa cggtgaacgt
     3481 gcccaggggg atggccacgt tgatattctt cagattgtta tgccgtgccc catggacttc
     3541 caaccaaccc ttgcctggcg gcgtcaaaga ccgccaaatc tttttccccg gtgagtcctg
     3601 gcgagaggca tcttcaagaa tcgccgcctc cgctaccttc tcgttccacc aggaaggcat
     3661 ccgacggttt ttgggaacgg ggatcgcctt ttgaccagat aggtaaggtc ccgtgacgga
     3721 tcgagtgtcg tggacaatgg tgctggggtg cccttccgcc acaatttggc caccatcttc
     3781 tcctgccgcc gggccgaagt ccaagagatg atctgccgaa ttgatgacct cccgatcgtg
     3841 ctcgaccact aacaatgtgt ttcccaggtc ccgcaacttg cgaagggcgt ccagtagccg
     3901 ccggttatcg cggggatgca ggccaatcgt cggctcgtcc agcacgtaca ggacgccaca
     3961 gaggccgctg ccaatttggg cagccaagcg aattctctgc gattcaccac ccgacagggt
     4021 cggtgccgag cgcgccaacg tcaaataatc caatcctacg tcaacgagaa actgaacacg
     4081 gttctcaatt tcccggacca cctcacccgc tatctgccgt tctcgctcat ccaactgcca
     4141 catgcggatt tccgcgatca aacgtcccag tgggaggcga caccactcgt caatggttcg
     4201 cccacggaaa cgaaccgccg aggcatcgtc ccgcaaacgg ctgccggcac aggcggtgca
     4261 ttcgacttcc gtaatgaaac tgctgagcac cgtccgcaaa ctcggcacac tactggcggc
     4321 agcctccaaa caaggataga gaccacgata ttgaaaccgt aacaacaact gcggactact
     4381 agcgtcctct ggggattgtt gcgaggattt tgtcccgtcc aatccctggg caatccgtgc
     4441 gtaatcctcc acgtaaacat cgaaccaacg ttcccctgtc ccgtggaaga tgagcctcct
     4501 ggcatggcta ggtaactccc caaacggcac gtcaccaggg atgccggtgt cgcggcaaaa
     4561 ggcctcgacc atggcacggg ccagtggttt attggtccgc ggccaaatca ccaaggcctg
     4621 atccagcaga ccctttttcg gatcgaggat cagggccgcc gggtcggccc ccatttccac
     4681 gcccaggccc tgacatcccg gacaccaccc caaaggactg ttgaacgaaa agttgttcgc
     4741 cgataggggt ttgaagcttc gaccgcaacg atggcaggca aaatgttgac tgtgagtaac
     4801 caccttccaa aggggctcgg gaacgcgatc ccgaggatac aggacagcca ggacgccttt
     4861 ccccagggta agggcgttct ccacgctgcc ggcaatccga ctccgttggt cggggcgaac
     4921 aaccaaccga tcgaccacga cttcaacttg gtgtcggcgc cgccgatcaa ggcggggtgg
     4981 atcttccagg ctatgaatga caccattcac ccgaatccgg ctatagcctg cggcccgcaa
     5041 ctggttccat atctcgccga aatcagtttc gtcctgaatc tccaaaggcg ccaagatgta
     5101 aatgcgtgtg ccctcgggat gatcaaggac cttactcaca atctgatcga ccgtttgcgt
     5161 accgacggga acattgcact cgggacagta gggttgtccc aggcgggcca tcaggacgcg
     5221 gaagtaatca tagatttcgg tgactgtgcc gaccgtggat cgcggtgtgt tggccgcgtg
     5281 cttctgctca atggcgatcg ctggggaaag gccctcgacg cgatccacct tgggtttctg
     5341 catctggctg acaaactgtc gggcataggt gctaaggctt tccacatacc gccgctggcc
     5401 ctcggcatag atcgtgtcca tggccaggga ggtctttccc gatccacttc gcccgcagca
     5461 aaccgtcaac ttatcccgcg ggatttcaac gttgattcct ttgaggttgt gctgtcgggc
     5521 accccaaaca ctaaccgacg tgataggttt actttgttgc ctttgggttt tgaggctttc
     5581 gaagaaggcc ttacgcttct tttcctcgcc gttaccctgc agggtttctc cggctaaggc
     5641 catccgaaga aaccgacctg tgtgcgaggc ctctacctta gccaggtctt ccggggtccc
     5701 agcagcgaca acccaacccc cggcctcccc tccttcgggt cccaggtcga taatccagtc
     5761 ggccgttttg attacttcca ggttatgttc cacaaccacc acggtgttgc cggcttcgac
     5821 aaaactgtgt aacaccttga gaagcatctc gatgtcggca aagtgcaaac ccgtggtagg
     5881 ctcatcgagg atgtagatgg tccgtccggt gctacgtttg acgagttctc gtgccaattt
     5941 gactcgctgg gcctcgccgc cggacagtgt tggcgagggc tgccccaatt tcatgtaatc
     6001 cagccccacg gcgtggaggg tttccaattt gtgccgaatg ggagggatat tttcaaacag
     6061 ttctagggcc tcgcggacct ccatttccaa aacgtcggcg atcgacttcc ccttgtaaag
     6121 cacctgtaat gtcagccgat taaagcgttg tccgttgcaa acggggcacg taacccaaat
     6181 gtcggccaaa aagtccattt ccagcttcgt tgccccgtga ccttcgcatg cttcgcaacg
     6241 tccgcctttc acgttgaagc taaagcgccc cggcttatac ccgcggacct tcgcctccgg
     6301 caaactggca aacagtttgc ggatgtcatc gaacaatttg gtgtaagtgg cgggattgga
     6361 acgcggtgtc cgcccgatcg gtgattgatc aatagcgatc atcttgtcca gatgttccag
     6421 cccctcgatc cggtcaaagg cgccgggctg ccccactcca ccattgaggt cccgatgaag
     6481 ggcctccacc aggatgtcgc taaccaaaga actcttgccc gacccggaga cacccgtgac
     6541 acatacaaac aagcccaacg ggatttccac gtcgatgttc ttcaggttat tgtgccgtgc
     6601 cccgcgaatg atcaactttt tgtccgtggg ctggcgacgc ttggtgggca caggaataca
     6661 gcgtttcccc gtcaggtact ggccggtcag gctgtcgggt gatttaacga tatcctcgaa
     6721 actgccagca gccacgatct gtccgccgcg actgccgggc ccaggaccaa aatccacgat
     6781 atagtctgcc gcccgaattg tatcctcatc gtgttccacc acgacaactg tgttgccctg
     6841 gtcacgaagt ttggccagtg ttgccaagag ccggtcgttg tcgcggggat gcaacccgat
     6901 ggatggttcg tccaaaacgt aggtgacgcc caccaggcca gaaccgattt gactggccaa
     6961 gcgaatccgc tgcatctccc cgcccgaaag gctcggcgcg gagcgatcca acgtcaaata
     7021 atccagcccc acgttcaaaa ggaagtcgag acgattgcgg atttctttga ggggctcttc
     7081 agcgatcttc tgctggatct gatccaaaac caaatcggta aaaaacaggc gtgtgtcagc
     7141 aaccggcaaa gcgcaaacct cgggcagtga tcgtgaaggg ttatcttcaa atcgcggatg
     7201 cgacgtctgc aagcgaatga atcgtgcctc cggtcgaagc cgagatccct ggcatgaggg
     7261 acataccgac acacgtcgcg cctcttgcaa cttttccctg gcagaggctt gcaccagcgt
     7321 ttcgggcaac acctcaagaa tgccggcaac cccgcccgtt atgcgagagt tccacaccaa
     7381 tttctcctta accgcccatg ggacgttccg ggcattctcg ccgaaaagga gaatctcttg
     7441 aatctgttcc ggcaattctt cccacgccgt ttccaagagt gccccttttt cataacctaa
     7501 ctcctcctca aggattgacg cccaccgccg caaaaggtag cggacgtccg gcaacatctc
     7561 cttccacctt cccaacgcag aaatgacgcc ctgctgaatg gagcgatggg ggttctcaat
     7621 gaatttccgt ggatcgaccg tctcaacctg tcccaaccct tggcaccgct gacacattcc
     7681 ttcgggacta ttaaagctaa acaattgtgg cgtcggttgg gcaaagctga tttgacagtt
     7741 gggacaggca aatcgtgaag aaaaccgccg gaccaggccc ctcgcggttc cgcgttgctg
     7801 cgaaccgtct gtcgcctcat tcccctcgca aacgaccatc acctcgccgt cgctcagacc
     7861 tatcgccgac tcaacggcct cggccaatcg actccgctgc gcggggctaa cctccagccg
     7921 atcaacgacc agctcgatgt cgtgccgcat gtgtcgtcgc agacggaggt cttgatcgag
     7981 ccggacgact tctccatcaa ttcgggcacg ggcaaaaccg cgccgacgca acgtggccag
     8041 caggctttga tactcaccct tctgaccgcg gatcaacggg gcgaggacca aaaccgtctt
     8101 tcctgtaaac gactccagaa tctgctcgac gatcagttgc cgcggctggg cttgaatccg
     8161 ctctccacat tgcggacagt acgccgtccc cgcccgcgca tagagaattc ttagaaaatc
     8221 gtaaatctcc gtaattgtgc ccaccgtcga gcgtggatta gacccagccg ttttctggga
     8281 tatgcagatt gaggggctta ggccctcgat ccgatcgacg ttgggtttgg gcatctggcc
     8341 gatgaattgg cgggcgaagg tggagaggct ttcgatgtaa cgccgctgtc cttcggcgta
     8401 aagggtatcg aaagccagag aacttttccc agagccgctg actcctgtca ggcagatcag
     8461 atgattgcgg ggtaagacaa tattgacatt gcgaaggttg tgctcccgtg ccccttgcac
     8521 aacgatatcg gaggcagcca tgcgagatta cccgaaaa
//
LOCUS       DAOSUT010000169         8480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4358_length_8480_cov_9.023501, whole genome shotgun sequence.
ACCESSION   DAOSUT010000169 DAOSUT010000000
VERSION     DAOSUT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_8480_cov_9.023501"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2365
                     /locus_tag="PLD05_13125"
     CDS             <1..2365
                     /locus_tag="PLD05_13125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOP78430.1"
                     /translation="ADLGDGVAVIMSLLGGIVVGMTFLLGRSRLTIRLGWADAAVLLV
                     VLSAIVASIRGALVGTARPAINMAWEWLGYGLIWLLIRQLRLNGRERRLILAGLASIA
                     VGAASYGLYQYLVEFPAMREAYQANREEWLRSLGWDALQTNPTWLGQFENRLYSREPL
                     ATFALANSLAGFILPWCVLLLGAFLQSGIIGRSSTHLTDPQNPAEKPRPPGRSQHTRF
                     SLAQLSRLGRLLWPVGTLIPLSLAGVCLVLTKSRSAYLALLIGIIVLLGASRALRQRQ
                     WRLARFVVISLLVAATGIAVFLRGLDWAVLSEAGKSLSFRWQYWVATVQMIKDHPLFG
                     CGPGNFRYAYLHYKVPEASEEVSDPHNFVLELTATAGIPAGLIFSMGLLATMGQLVRL
                     GWCGHSGREPVSTVAANAVSENAKERQVSPWTAEILALGAGLLLAWPVGWLFGLLSSA
                     PQTATFILVGCPVAILSLITLWPWIENGQWTPLIALVSLVALSVHWLAAGGLTFPGVA
                     YSFWLLWALAQDVIEETSRAVSTSPNAQPPSHKWTFPWGASTKEVPVSKVGAFAAFGA
                     MAGAGLLCYFTAYAPVLDARGLMAEAARIKAVFPPRAEELLRAAAKADPWAYEPWLRR
                     LELWVNTIAPSDNRDNSPRAKTAATPSAIIQPLWADPTAELDECAVNLLRRAKNHHVV
                     RWNLAIAYQKLAQVTGSADPLHRAIKYAADAIRLYPTQAIYYVGYADMLRQAGRLREA
                     RQAAQKALWLHEVTPHQDRKLPDETVTSLNAILKLDPNKETDRQPPP"
     gene            complement(2423..3238)
                     /locus_tag="PLD05_13130"
     CDS             complement(2423..3238)
                     /locus_tag="PLD05_13130"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP78431.1"
                     /translation="MARWLFKASYARPLVCRLGVIGLLSVLCCVGLMAVAEEASVQDT
                     PEVIRLFDGQTLNGWVVKCKPQDNDKRGYWKVVDGTIMADVPPGSPHNYIWLMTEKEF
                     ADFELCLKVQTFSTSTGNSGVQVRSRYDDEAYYMDGPQVDINPPGPWRCGFIYDETRG
                     AQIWLWPDVGRPANAKPEHAPKGWKWFHADHPDTKDAWNDLRIVCHGFRIQTFVNGVP
                     IADYMGQGRLDDDAHQLRRVGLTGHIALQIHPGGTLQIRFKDILLRPIRNAGE"
     gene            3526..4065
                     /gene="ilvN"
                     /locus_tag="PLD05_13135"
     CDS             3526..4065
                     /gene="ilvN"
                     /locus_tag="PLD05_13135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOP78432.1"
                     /translation="MRHILTALVQNVPGVLSHIAGMLASRGYNIDSLAVGETEDPRLS
                     RMTFVVVGDDAVIEQVRKQLEKIVTVVRVDDVSSRDYVERDLMLIKVAAPHEKRGEIR
                     ELVEIFRGRIVDVAPQSMIIEISGQEKKVESFIELMRPFGILELVRTGRIAMVRGETT
                     LKFDENNNGQEYLVSHLAR"
     gene            4113..5117
                     /gene="ilvC"
                     /locus_tag="PLD05_13140"
     CDS             4113..5117
                     /gene="ilvC"
                     /locus_tag="PLD05_13140"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOP78433.1"
                     /translation="MPAKIYYDEDADLSLLKGKTVAIIGYGSQGHAHAQNLRDSGVRV
                     IVAELPGTANYKLAVEHGFQPMSAEEASKQADIITILLPDEIQPDVFRSSIRPNLRAG
                     NVIVCSHGFNVHYGQFDIPDGVAAVLVAPKGPGHLVRSEFVKGGGVPCLVANGPNGTE
                     ETFKLALAYAKGIGGTRGGVIETTFAEETETDLFGEQVVLCGGVSELICAAFETLVEA
                     GYQPELAYFECMHELKLIVDLMYQGGLSYMRYSISNTAEYGDYTRGPRIINQQTRAEM
                     KKILAEIQSGAFAKEWILENKANAPMFKALRKKARAHMIEEVGRKLRKMMKWIDAKEV
                     "
     gene            5349..6431
                     /gene="hypE"
                     /locus_tag="PLD05_13145"
     CDS             5349..6431
                     /gene="hypE"
                     /locus_tag="PLD05_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOP78434.1"
                     /translation="MSDSSADFSRFHCPTPLATHETIQLAHGGGGRLMRQLINEVFLP
                     AFRREGDTEGLRQPPHDSAEIRLGDGTRMAFTTDAFVVSPLFFPGGDIGKLAVFGTVN
                     DLAMSGAKPLWLSASFIIEEGLPMEILSQIVRSMREAADEASVRIIAGDTKVVDRNKG
                     DGLYISTTGLGWIPEDVHISPAHIRPGDKVILSGDIGRHGIAIMSVREGLQFDNALPS
                     DCAPLHRIVRAMVKHNARAVHCLRDLTRGGLATALNELAQDAHVGFVIDESRITVMPP
                     VASACEMLGLDPLYVANEGRFVAIVSPEAADDLLEVMRGQGEAVGLQIIGEVRAQPAG
                     VVELRTAYGSSRILDWLSGEQMPRIC"
     gene            6491..7126
                     /locus_tag="PLD05_13150"
     CDS             6491..7126
                     /locus_tag="PLD05_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78435.1"
                     /translation="MYQVLCCGMFILAATGLSVYGQAEEASPIFADSFREKLAEGWAW
                     LRELPGGWRIREGGLEIRALPGDANTVKNALLRPAPDRTAGPFAVEVTVSNVTPPTVQ
                     YEQVGITWYVDEKPVFKLVKELIDGKIYIIPGRKPVENVPIRLRLVIDGDRWTAQYRP
                     AGEKEFLVAAEGTLPPPANDHVSLQCYHGPENAEHWMRFEDFRILRLPSKR"
     gene            7302..8060
                     /locus_tag="PLD05_13155"
     CDS             7302..8060
                     /locus_tag="PLD05_13155"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP78436.1"
                     /translation="MTRCAAILGGLLTVCLLASGTWAESTSSAQGGKIRVLLTYGGHG
                     FEEKPFFEMFDNMEGIEYKAIKLPDQADMLNPKAADEFDVIVMYDMVPAISEKQQEAF
                     VALLKKGIGVVSLHHNMGAHRNWDEFRKIIGGKFILEDCVIDGQEYKKSGWAHGQDYK
                     VIIADPNHPITRGLKDFIIHDETYKDYYTAPNVHVILKTDCPTNDPEIGWVTKYGESR
                     VFYLMLGHDSLAWKNPNYPVILKRGIEWAAGRLQ"
     gene            8128..>8480
                     /locus_tag="PLD05_13160"
     CDS             8128..>8480
                     /locus_tag="PLD05_13160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP78437.1"
                     /translation="MAQDQRVVVITGVSRGLGRAMVDRFIALGHRVAGCARSKSSVDE
                     LSTIYGEPHLFRVADVSNEASVAAWAREVIDRLGPPDLLINNAALINRNARLWEIGAE
                     EFQALLRVNVGGIHLT"
BASE COUNT         1836 a         2205 c         2478 g         1961 t
ORIGIN      
        1 tgccgacctc ggggacggcg tggccgtgat catgtccctt ttaggtggga ttgtcgtcgg
       61 tatgacgttt ctgctgggca ggtcccgact gacgattcgc ctgggatggg ccgacgccgc
      121 ggttctcctt gtggttctgt cggccattgt ggcctcaata cggggagccc ttgttggcac
      181 tgcccgaccg gccatcaata tggcgtggga atggctaggt tacggcttga tctggctcct
      241 catccgccag cttcgattga acggacgaga gaggagactt atcttggcag gcctggcctc
      301 catagctgtg ggcgcggctt cgtacgggct gtaccagtat cttgttgagt ttcccgcgat
      361 gcgggaggcg tatcaggcga atcgagagga gtggctgcgt tccctggggt gggacgccct
      421 gcaaacgaat cccacttggt tagggcaatt tgaaaaccga ctttacagcc gtgagccgct
      481 ggccacattt gccctggcca attccttggc gggattcatc cttccttggt gtgtcctcct
      541 tctgggggcc tttctccagt cagggattat cggaaggagc tcgactcacc tgacggatcc
      601 acaaaatcca gctgaaaagc cacgcccgcc ggggagaagc cagcacactc gtttcagtct
      661 agcccaattg tcccgtcttg gtcgtctcct ctggcctgtt ggcacgctga tacctctcag
      721 cctagcgggg gtgtgtttgg ttctgaccaa aagccgctcg gcctatcttg ccttacttat
      781 cgggatcata gtcttgcttg gggcaagccg agctttacgg caaagacagt ggcgacttgc
      841 ccgtttcgtt gtgatcagtc ttttggtggc cgcgacgggc attgccgttt ttttacgcgg
      901 tctcgattgg gctgttctct ccgaggccgg aaaatcattg agctttcgct ggcagtactg
      961 ggtggcaacc gtgcaaatga ttaaggacca tcccttattc ggatgtggtc cgggcaactt
     1021 ccgttacgct tatcttcact acaaagtgcc agaggcgagt gaagaggtct ccgatccgca
     1081 caattttgtc ctggagctaa cggcaactgc gggcatccct gcgggtttga tcttctccat
     1141 ggggcttttg gcaaccatgg ggcagcttgt cagactcggt tggtgtggac acagtgggcg
     1201 tgagccggtt tccaccgttg ctgccaacgc tgtgagtgaa aacgccaagg agcggcaagt
     1261 gtccccctgg acggccgaga tcttagccct gggggccggt ttgttgttgg catggccggt
     1321 tgggtggttg tttggcctgc tcagctcggc cccacaaacg gcgacattta ttctcgtggg
     1381 ttgtcccgtg gcgatcctta gcctgataac attgtggccc tggatcgaga acggtcagtg
     1441 gactcccctc attgcgttgg tcagtctggt ggccttatcg gttcattggc tcgcagcagg
     1501 agggctaaca tttcccggtg tagcgtattc gttttggctc ctttgggcct tggctcagga
     1561 cgtgatcgag gagacttctc gtgcagtttc aacctcgcca aacgctcaac caccgtctca
     1621 caagtggact ttcccgtggg gagcgtccac gaaggaggtc ccggtatcaa aggtcggggc
     1681 gttcgcggcc tttggggcga tggcgggtgc ggggctcctt tgttatttca cggcgtatgc
     1741 tcccgtgctt gatgcccgtg gtttgatggc cgaagcagcc aggattaagg ccgtttttcc
     1801 tcctagggca gaagaacttt tgcgtgccgc cgccaaggct gacccctggg cttacgaacc
     1861 gtggctgcgg cgactggagc tttgggtgaa tacgattgcg ccttcggata accgcgacaa
     1921 ttcaccgagg gcgaaaacag cggcgacgcc aagtgcaata atccagcctc tttgggctga
     1981 tcctacggca gagttggacg agtgcgcggt gaatctgctc aggcgggcga aaaaccacca
     2041 cgtcgtccgc tggaatttgg cgattgcata tcaaaaactg gcgcaggtga cgggaagcgc
     2101 agacccgctt catcgtgcaa ttaaatatgc cgcggacgcc atccgtcttt atcccactca
     2161 ggccatctac tacgtcggat atgccgacat gctccggcag gcgggacgtc tccgcgaggc
     2221 ccggcaggcc gcccaaaagg ccctttggtt gcacgaggtt acaccccacc aggatcgcaa
     2281 attacctgat gaaactgtta ccagcttgaa tgcgatcctc aagcttgatc caaataagga
     2341 aaccgaccgt cagccgccgc cttaagtcgt ctccaggttg ccttcgattt cgcggtaaac
     2401 ccctgaagag aaggaaccgc gctcattccc cggcattgcg gatcgggcga aggagaatat
     2461 ccttaaagcg aatttggagg gtacctcccg gatgaatttg cagggcgata tggccggtca
     2521 atcctacccg gcgaagttgg tgggcgtcat catcgagtcg cccttggccc atgtaatctg
     2581 cgattggtac accgttgacg aatgtctgaa tccggaaccc gtggcacacg atgcgaagat
     2641 cgttccaagc atcttttgtg tctggatgat cggcgtggaa ccatttccat cccttcggcg
     2701 cgtgttctgg tttggcgttg gcgggacgcc caacgtcagg ccatagccaa atttgtgccc
     2761 cccgtgtttc gtcatagatg aagccgcaac gccatggacc aggcggattg atatccacct
     2821 gtggcccatc catgtaatat gcctcgtcgt catagcgact ccggacctgc accccactgt
     2881 taccggtgga agtggagaaa gtttggacct ttaggcaaag ctcaaaatca gcgaactctt
     2941 tctcggtcat gagccaaata tagttatggg ggctgcctgg agggacgtct gccatgatgg
     3001 tgccatcgac gaccttccaa tagccacgtt tgtcgttgtc ctgtggcttg cacttgacga
     3061 cccagccgtt tagtgtctga ccatcaaaca accgaataac ctccggcgtg tcctggacgc
     3121 tggcttcctc tgcgacggcc attaacccta cacaacagag gaccgataac aagccaatca
     3181 cccccagccg gcagaccaac ggccttgcgt aacttgcctt aaagagccat cttgccatgt
     3241 gctaagtcct tttcttgaaa cgggcagatt tcggtatgtc gtaaataaag catttcgtat
     3301 tatgatgcca aagctcggag gcaaaatcaa aatgatgccc taaaacccgg ccgaccgaac
     3361 ggtaacacgc gatccaagcg aaccttacgc tcagatgaca tgtccgactt gagggggact
     3421 ctctgaaacc ggcagacggg ctccctgacg ccggacggct tgtcattccc cttaaggagc
     3481 gttaggatag tcattttacg cagaaagcag gagtcatcag cggttatgag acacatcctg
     3541 acagcacttg tgcagaacgt gcctggggta ctctcgcata ttgcggggat gctcgcctcg
     3601 cgcggatata acattgacag cttagccgtc ggtgaaaccg aagacccgcg gctttctcgg
     3661 atgacattcg tggttgtggg tgacgatgcc gttatcgagc aggtacgaaa gcaactggaa
     3721 aagatcgtca cggtcgttcg cgtggacgac gtaagcagcc gcgattacgt cgagcgagac
     3781 ttgatgctga ttaaggttgc ggccccgcac gaaaagcgag gcgaaatccg cgagctcgtg
     3841 gagatcttcc ggggacgcat cgtggatgtg gctccgcaaa gcatgattat cgaaatatcg
     3901 ggccaggaaa agaaggtgga atccttcatc gagttgatgc gccccttcgg cattctagaa
     3961 cttgttcgga ccggacgaat cgccatggta aggggcgaaa cgacgttaaa gttcgacgaa
     4021 aataacaacg gtcaagagta cttggtgagt cacctcgcgc gctaagcgct aagagtcggg
     4081 ataacagtcc aagataaaca ggagtttccg tcatgccggc gaagatttat tacgatgaag
     4141 atgccgattt gtcgctttta aaagggaaaa ccgtggccat catcgggtat ggctcccagg
     4201 gacatgccca cgcccagaat ttgcgtgaca gcggtgttcg tgtcattgtg gccgagctgc
     4261 ccgggacggc caactacaag ctcgccgttg agcacgggtt ccagcccatg tcggcagagg
     4321 aggcaagcaa gcaggccgat atcatcacca ttttgctgcc ggatgaaatt cagcccgatg
     4381 tgttccgcag ttcaatccga ccgaacctac gggcggggaa tgtgattgtg tgttcccacg
     4441 gcttcaatgt tcattacggg cagttcgata ttccagatgg cgtggcggcc gtcctcgtgg
     4501 cacccaaggg gccggggcat ctcgtgcgat cagaattcgt caaaggcggt ggtgtcccct
     4561 gtttggtcgc caatggtccg aacggcaccg aggagacatt caagctcgct ttggcttatg
     4621 ccaaaggtat cggcgggacc cgcggcggcg tgatcgagac cacctttgcc gaggaaaccg
     4681 agactgattt gttcggcgag caagtcgtcc tctgcggtgg cgttagcgaa ttgatttgcg
     4741 ccgcttttga aacgcttgtg gaggctggtt atcagccgga gttggcctat ttcgaatgca
     4801 tgcacgagtt gaagctaatt gtggatctaa tgtatcaagg tggtctgagc tacatgcggt
     4861 atagtatctc caataccgca gagtacggtg actacacgcg aggaccgcgt attatcaatc
     4921 agcaaacgcg ggcggaaatg aaaaagattc tggccgagat tcaatccggg gcctttgcca
     4981 aggagtggat tctggaaaac aaggcgaacg cccccatgtt taaagctcta cggaaaaagg
     5041 cccgcgccca tatgattgag gaggttggcc ggaagctgcg aaaaatgatg aagtggatcg
     5101 acgccaaaga ggtttgatct ccaccgtttg attcattggt ctgtcgctcg cccaaacttg
     5161 ctccctagca ggtttgggtt ttttctttct tgtggaccga gggccatgcc acggtcgagg
     5221 acatagcggt ctggccaccg attcgcctat aatgttgtgg tgcctgtgga tgaaagcctg
     5281 gtcttcagga aattgcttct tagatgcttt atttgcgaag aacacttcaa atacgatagg
     5341 gttgatctat gtcagattcg tcagccgatt tcagccgttt tcattgtcct acgcctcttg
     5401 ccacgcatga gacaatccaa ctagcccacg gcggtggtgg gcgactgatg cgacagctca
     5461 tcaacgaggt gttcttgccc gcttttcgcc gtgaaggtga caccgagggt ctaagacaac
     5521 cgcctcatga cagtgccgaa attcgcctgg gggatggcac tcgcatggcc ttcacaacgg
     5581 acgccttcgt ggtgagccca ctcttttttc ccgggggtga cattggcaaa ctggcggtgt
     5641 ttggaaccgt caacgacctg gcaatgtcgg gggcgaaacc attatggttg agtgccagct
     5701 ttatcatcga ggaaggattg cccatggaga tcctctcgca gatcgtcagg tccatgcgcg
     5761 aggccgccga tgaagcgagc gtccggatca ttgccgggga tacgaaggtt gtcgatcgga
     5821 ataaggggga cggactttat atcagcacaa ccggcctagg atggattccg gaggatgttc
     5881 acatttcgcc ggcacatatt cgaccggggg acaaggtcat cctctccggc gatattgggc
     5941 gccacggtat cgccattatg tcggtgaggg aagggctaca gtttgataac gccttgccga
     6001 gtgattgcgc accacttcat cggattgtgc gggccatggt caagcacaac gcgcgggccg
     6061 tgcattgcct tcgggatttg acccggggtg ggctggcaac cgcgctcaac gagttggcac
     6121 aggacgccca tgtggggttt gtcatcgacg aatcgcgcat cacggtgatg ccgcccgtcg
     6181 catctgcttg cgaaatgctg ggactggacc ccttgtacgt ggccaacgaa ggccggttcg
     6241 tagccatcgt cagccccgaa gcagcggatg atctcttgga agtgatgcgg ggacaaggcg
     6301 aggccgttgg actgcagatc attggcgagg tgcgggcaca accggccgga gttgtcgagc
     6361 ttagaacagc ctacggcagt tcccgaattc tcgattggct ttccggagag caaatgccgc
     6421 ggatttgtta gcttacgcca gcgtgaccgt ttccttgccc atatgtagga catcaatgag
     6481 gggatacacc atgtaccaag tgttatgctg cggaatgttc atcctcgctg ccaccggttt
     6541 gtcggtttac ggacaggctg aggaggcatc tccaattttt gccgattcat tccgagaaaa
     6601 actggccgag ggatgggcct ggctgcgaga actgcccggt ggctggagga tccgcgaagg
     6661 tggccttgag atacgcgcct tgcccggcga cgccaatacg gtcaagaacg cccttctgcg
     6721 gcctgcgccc gatcggacgg ctggaccatt tgccgtggag gtcacggtat caaacgtcac
     6781 gccaccaaca gtgcaatacg aacaggtggg cattacgtgg tacgtggatg agaagcccgt
     6841 tttcaaattg gtcaaggaac tcattgatgg taagatatac atcattccgg ggcgaaaacc
     6901 cgtcgaaaac gtgcccatcc gcttgcggct cgtaattgac ggagaccgct ggacggcgca
     6961 atatcgtcct gccggtgaaa aggagttctt ggttgcggcc gagggaacct taccaccgcc
     7021 ggccaatgac catgtcagtc tgcaatgtta tcatggtcct gagaatgcag aacactggat
     7081 gcgattcgaa gactttcgca tcctccggtt gcctagcaaa cgttgatcga aagaacttaa
     7141 cggggtggga ggaagttggc actgaggaat gatcttcggt gtcgtcacgc aagtttaacc
     7201 atttttaaat caccaaaaat cacacctaaa tacctacgtg ttttggcaga gtattgtagg
     7261 aatcctgtgt tgacgggctt tgtccttgag gaggtcgaac gatgacacgg tgcgcggcta
     7321 tcttgggtgg tttgttgacg gtatgtctgc tggccagtgg gacttgggcc gaatcaacga
     7381 gctccgcgca agggggaaag attcgtgtcc ttttgacata tggcggccac ggcttcgagg
     7441 aaaagccatt cttcgagatg ttcgacaata tggaggggat cgagtacaaa gccatcaaat
     7501 tgccggatca agccgatatg ctcaatccca aggcagcaga cgagtttgat gttatcgtca
     7561 tgtacgatat ggttcccgct atttcggaaa agcaacaaga ggcgttcgtt gccttgctca
     7621 aaaagggcat cggtgtggta tcgttacacc acaacatggg tgcccatcgt aattgggacg
     7681 aattccgaaa aatcatcggt ggcaagttca tcctcgaaga ttgtgtgatt gatggacaag
     7741 agtacaagaa atcaggctgg gctcacggcc aggattacaa ggtgatcatt gctgatccta
     7801 accatccaat cacccgggga ttgaaagatt tcatcattca cgatgaaacc tacaaggatt
     7861 attacacggc ccccaatgtg cacgtcatct tgaaaacgga ttgcccgacg aatgaccctg
     7921 aaatcggttg ggtcaccaaa tatggagaaa gccgggtatt ctacctcatg ttggggcatg
     7981 atagcctcgc ctggaaaaac cccaattatc ccgtgatcct caaacggggg atcgaatggg
     8041 cggcaggtcg gctccagtga gagccgtgtt ctaccgttaa cgattctcaa gatgtcggtt
     8101 ccttatcatt gggaataggt cgccaccatg gcacaggatc aacgtgttgt cgtaatcacg
     8161 ggcgtcagtc ggggtctggg gcgggcaatg gtcgatcggt ttatcgccct cgggcatcgt
     8221 gtggctggtt gcgcccgatc taagtccagc gtcgatgaat tgagcacgat ctatggcgag
     8281 ccacatctct tcagggttgc cgacgtgtcc aatgaggcat ccgtggcagc ctgggcccga
     8341 gaggtcattg atcggcttgg gcccccagat ctcctcatca ataatgccgc cctcattaat
     8401 cgaaacgccc gactgtggga aattggagcg gaagagtttc aggcccttct gagggtaaac
     8461 gtgggtggaa tccatctcac
//
LOCUS       DAOSUT010000170         8468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4363_length_8468_cov_9.569951, whole genome shotgun sequence.
ACCESSION   DAOSUT010000170 DAOSUT010000000
VERSION     DAOSUT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_8468_cov_9.569951"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(837..1346)
                     /locus_tag="PLD05_13165"
     CDS             complement(837..1346)
                     /locus_tag="PLD05_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOP78438.1"
                     /translation="MDVLTAIFTRRSIRTFEERPVPEEMRETLLRAAMAAPSARNAQP
                     WQFVVIDHRATLMEIAARFPNAQPARTAPLGILVCGDLSLELSKGYWVIDCAAATQNI
                     LLAAHGLGLGAVWTGVYPRENRVEGLREMLGIPEHVVPHSLVLIGYPAESPAQVDRFD
                     AKRIHYNRW"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1623..2192
                     /locus_tag="PLD05_13170"
     CDS             <1623..2192
                     /locus_tag="PLD05_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOP78439.1"
                     /translation="SGYWEEARRVIRHFVKVFRPYRYIVCPSGSCSAMCRVFFAQADP
                     DPATTEVGSRVFELTEFLVNILGVTDLGARFPHKVTMHSGCHGRRELGIFEQPISLLK
                     NVKDLVYCELPNIEECCGFGGTFSVKMPGTSIAMGETKAANILRSGADVVTSVDISCL
                     MHVGGIMRRKEEMRHIRVMHIAEILNTRE"
     gene            2245..3675
                     /locus_tag="PLD05_13175"
     CDS             2245..3675
                     /locus_tag="PLD05_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOP78440.1"
                     /translation="MKAVHSQHVSLQGAAFWKAENKGLAEPRGSKALGESAERSSKHN
                     RQIAMAIPYWEQWREAAHQIKRYAIDHLDDLLVQFEKNLIARGCTVLWAKDAAEANQH
                     LLNIVKEHNVKTVVKSKSMVSEEMEVNHVLASVGVKALETDLGEFLVQLAGQRPTHIV
                     TPALHLSASDVGRLFAEKLGEPYTAEHEKLTDIARRHLRQEYLRADMGISGCNFGLAD
                     TGAICIVENEGNAGLSISAPPVHVALIGIEKVIPSIEYLPQFLNLLGRSGTGQKLTTY
                     THIVHGATPGRKFYVILIDNGRSNVLKDPAARTALFCIRCGACLNACPVYRRVGGWAY
                     GWVYPGPIGSILTPHLVGFDKAGKLPFASSLCGACGEVCPVKIDIPHQLVHLRHRAVN
                     TRSPVRSPLERLIWNAWAWAMRGPLRYQLTMGFVRLGVRLAKFLPFHPAQLGAWTRGR
                     ELPLVPSTTFRSWWRRHGKRFEQPAG"
     gene            3743..4396
                     /locus_tag="PLD05_13180"
     CDS             3743..4396
                     /locus_tag="PLD05_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOP78441.1"
                     /translation="MNSRDIILQRIRENLGPRKEEAPPPVPTVWPRSSVDRATLARRF
                     AEELQAVHGQAVLCPSWDAACQDFQRLVRDSGWQQGVVMDRPEAWRLASGVSGFQAFQ
                     VSENPPPRDMEPVPVSILAADWLLADTGSAVVICNQPGERLACYLPPACVIVATADKL
                     REHLPAAWEEIVPLARQPERRGEFVIITGPSRTADIEKILILGVHGPKRLIVYVVGE"
     gene            complement(4460..8044)
                     /gene="dnaE"
                     /locus_tag="PLD05_13185"
     CDS             complement(4460..8044)
                     /gene="dnaE"
                     /locus_tag="PLD05_13185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOP78442.1"
                     /translation="MATPPFVHLHCHSHYSLLDGAGTVDRLLDRARDLCMTALALTDH
                     GNLFGALEFYQKAKDRGIKPIVGYEAYVAPGSRFQREAASMKEASYHLTLLAQDKRGF
                     QNLLRLASAAYLEGFYYRPRIDKELLAQHHEGLICLSGCPSGELSRTLLAKEDPDIAR
                     ACEVADWFRSLFGDRYFIEIQDNGTEIQRLAMKGAIAVAKKMNIPLVATSDVHYVMPE
                     DADAQDVLLCINTGKFRSDTNRMKMDGREYYLRSPEQMYQVFSGLEEAVRRTQDIASM
                     VDLELELGRRHFPIYTPPDGKTAEQYLRELCIEGLKERYRHDPRRFRNGELAPEVMER
                     LEKELYVINTLGFANYFLIVWDFVRFARSKGIQATARGSGVGSLVSYALKLSHVCPIE
                     YGLLFERFLDLNRREAPDIDIDFCQERREEVLQYVKEKYGHNNVAQICTFGTMAARAA
                     IRDVGRVLGHPVAFVNEITALVPEGPKVTIQAALEKSEDLRKRYETDLRVREVIDYAK
                     QIEGLARNVGTHAAGVVIADKPLVEYVPLQHVPGKEEVITQWAMADVERAGLLKMDFL
                     GLRNLTILSKVIGLIEQTTGEKVDPYSFPLDDTKTYELLCRGETKGVFQLESGGIREL
                     LQRMKPDNIRDIIAVNALYRPGPLEGGMVDDYIDVKHGRKKPEYKHPVMKEILEETHG
                     VMVYQEQVMQILNRLGGIELADAYTCIKAISKKKLDKIAKYREQFVKGACEKGLTQKE
                     SEELFTLIEKFAGYGFNKSHSTAYARIAYITAYLKAHYPLQFMAALLSSDIVNRNFTR
                     KDSLVEHLEDCHRMGIEVIPPDINLSEVEFTVKDGKIVFGLAAIKGCGGSAAEAIVEE
                     RRRHGPFRTLFEFCERLEQGSVNRSAIESLIKAGAFDKLGGHRAQLLAVLERAIQAGA
                     SAAEDRRRGQLSLFGNNSTRNPQVVDQQLPDIPEWPDHERLAKEKEVLGFYFSSHPLR
                     EYEELLGRYCTHTTADLGQLAHGTEVVVGGVVSAIVTKHTRAPRNDGPTKFAMFDLED
                     TAGIVRCILWPEPYTRFGEYLHDDAVLVALATVDKRGGGEEATLIIQELVPIAELKRR
                     FTRGIQIRFSEAQHNEVTLQNLYQIVRDHPGSCQLQLMLQLRDGRRVVMACDGMGVTL
                     DTDLEQQIEKLLGQQAICYLAGRFSLRNGNGNGNGHANGKNGSMRRSR"
     gene            complement(8232..>8468)
                     /locus_tag="PLD05_13190"
     CDS             complement(8232..>8468)
                     /locus_tag="PLD05_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP78443.1"
                     /translation="PIHELVPPDLPEDLSPAQLAQLTELLQYFHIRLRRLVQAASMDH
                     ASESVTLEQRHWQGLLDLYDRVAHYLRVIGEPPT"
BASE COUNT         1762 a         2328 c         2378 g         1900 t
ORIGIN      
        1 gttccgggga agtggttttt gtttttggtg aggccaggtt caatcgctgg gactgttgct
       61 cacccaagct gcccacaatt aaaccacttc ccgtcagaac cgtgacaatc aacaccaggg
      121 tcaggaagta atgacgtcgc acgctattca caaaggctga catggttacc ctcctatcgc
      181 ttaagtgatt ttatcagtta aggtccaaca acggccgcca gattttgaag caactcccac
      241 ctcttttcca cgatacacct acttatcgcg gaatcaggcc ccctagacgt ggcacgacag
      301 cgttctcgtt agcaggcttt tagaagatgt tgccagtccc cgatgccttg tagcaaggaa
      361 accttagagc cgattccaca acctcccctc gcccgtttgg cgggagaggg ggcgggggtg
      421 agggtggctc gaatcgctgc cggttttctc aatcgattcc gaggttcgct tgcgctcacc
      481 ccaaccctct cccagaggga gagggaattt tgggataatc tcttactaac tttggaaccg
      541 tttcttgccg gaatgggagg ccacctcctg gttatccttt tcctattttg ctcaattgcc
      601 tacattgcca agggtaaacc gtccaagggt tttcgcacag ctcacgcatc gtgaacctca
      661 tctgccaatc cggcgccgga acctgccaga atgggtttta ttttgtgcga gattcctggg
      721 acgatttacc tgcggtgggg ttactcagca attgctatgc cgaatttgcg agcccaaacg
      781 gttacgttgg cccgccactc ggcccgccgc actagacctg ccagcgcttg gtgcggttac
      841 caccggttgt agtgaatccg tttggcatcg aaacggtcaa cctgggccgg agattcggct
      901 gggtaaccaa tcagcacgag gctatgggga acaacgtgtt ccgggatgcc gagcatctct
      961 cgtaatccct cgactcggtt ttcccgggga tagacaccgg tccatacggc ccccagtccc
     1021 aaaccgtgcg ctgcaagaag aatgttttgg gtggcagcgg cacagtcgat gacccagtat
     1081 cccttagaga gttctaggct caggtcgcca cagacgagaa tccccagcgg tgccgtgcgg
     1141 gccggttgtg cgttgggaaa tcgtgcggca atctccatga gtgtggcccg atggtcaatg
     1201 actacgaatt gccacggttg ggcatttcga gcgctggggg cggccatggc agcccgcagt
     1261 agcgtttcac gcatctcctc gggaacggga cgttcttcaa aggtgcggat actccgtcgg
     1321 gtgaaaatgg ccgtcagaac atccatggtc attctccttg agccaaactc acgagactga
     1381 tctgtgccgg taaaaagagc ctacgttgtg atgcaccgaa agcttcatct ttggatagag
     1441 atcaccgcgg acctgcgcct gacatacgag tgggcaggtc atttcgcccg gcaatccgct
     1501 tgtttgggtt gatccaaaag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nntcgggcta ctgggaggaa gcccgtcgag tcattcggca ctttgtcaag gtcttccgac
     1681 cgtatcggta catcgtctgc ccgtcgggtt cgtgctcggc catgtgccgg gtctttttcg
     1741 cgcaggccga tcccgatccg gccaccacgg aggtcggttc ccgcgtcttc gaattgaccg
     1801 agttccttgt gaatatcctg ggggtgacgg acctcggtgc ccgatttccc cacaaggtga
     1861 cgatgcatag cggatgtcat ggtcggcgtg agctaggaat tttcgaacaa ccgataagcc
     1921 tccttaaaaa cgtcaaggac ctggtgtact gcgaattacc caatatcgag gagtgctgtg
     1981 gcttcggcgg tacgttcagc gtcaaaatgc cggggacctc gattgctatg ggggagacaa
     2041 aagcggccaa catcttgcgc tctggggccg acgttgtcac gtccgtggat attagttgcc
     2101 tgatgcacgt aggtggcatc atgcggcgga aagaagaaat gcgccacatc cgcgtgatgc
     2161 atattgcaga gattcttaat acacgagaat aaagattacc tgccggaaga tcggcagatt
     2221 caatggagaa aaaggcgagc gacgatgaaa gcggttcatt cccaacatgt gtcgcttcag
     2281 ggggccgcct tctggaaggc ggaaaacaag ggccttgcag aaccacgcgg gtctaaggcc
     2341 ttgggagagt cggccgagcg atcaagcaaa cacaatcgcc agatcgcgat ggccatccct
     2401 tattgggaac aatggcgaga agcggctcat caaatcaaac gctacgcgat tgatcatctc
     2461 gatgatctgc tggttcagtt cgaaaagaac ctgatcgcgc ggggatgcac cgtcctttgg
     2521 gccaaagacg ccgcggaggc caatcagcat ctgcttaaca tcgtcaaaga acataacgtg
     2581 aagaccgtgg ttaaatcgaa gtccatggtc agcgaggaaa tggaggtcaa tcacgtcttg
     2641 gcctcggttg gggtcaaggc cctggagacg gacttgggcg agtttctcgt ccaacttgcc
     2701 gggcagcgtc ccacccatat cgtgacgccg gcgcttcacc tctcggccag tgatgtgggc
     2761 cgactattcg cagaaaaact gggagaacct tataccgccg agcatgaaaa gctgaccgat
     2821 attgcccgcc gccatctgcg acaggaatac ctgcgggccg acatgggaat ctctggatgc
     2881 aatttcggac tggccgatac cggcgccatt tgcatcgtgg aaaatgaggg taatgccggt
     2941 ctgtccattt ccgctccgcc ggttcatgtt gccttgattg gcattgagaa ggtcatcccc
     3001 agtattgagt atctgccaca gtttttgaac ctgctcggtc gcagtggcac gggccagaaa
     3061 ttgacgacct acacccacat tgtgcacggg gccacgccgg gcagaaagtt ttacgtgatt
     3121 ctcattgaca acggacgttc caacgttttg aaggacccgg cagcccgcac ggccctgttc
     3181 tgcatccgat gcggcgcctg tttgaatgcg tgtcctgttt atcgtcgtgt gggcggttgg
     3241 gcctatggtt gggtctatcc ggggccaatt ggcagtatcc tcacgccgca cctggtgggt
     3301 tttgacaagg cgggcaaact ccccttcgca tcgtctttgt gcggtgcttg cggtgaggtc
     3361 tgcccggtca aaattgacat tccccatcag cttgtgcacc ttcgtcatcg ggcggtgaat
     3421 acccgatcgc cggttcggtc cccgttagaa cgtttaattt ggaacgcctg ggcctgggcc
     3481 atgagaggac cgctccgtta tcagttgacc atggggtttg tccgccttgg tgtgagactg
     3541 gccaagtttc tgccatttca cccagcacaa ttgggggcct ggacgcgggg gcgggagttg
     3601 cctttggtgc cgtcgacgac gtttcgctct tggtggcggc ggcatggaaa gcgtttcgaa
     3661 cagcctgctg gctagggtgc caccaattcg tagggggctt gcattcagtc cgtgacgtga
     3721 cataaacgac gagcgataaa ccatgaacag tcgtgacatc atcttgcaaa ggatccgaga
     3781 gaacttaggg ccgcggaaag aggaggcccc gccaccggtt cccacggttt ggcctaggag
     3841 ctcggtcgat cgggcgacgt tggcccgacg atttgccgag gaactccagg ccgtgcatgg
     3901 ccaggccgta ttgtgcccat cgtgggatgc tgcctgtcag gattttcagc ggctggtccg
     3961 cgattccggt tggcagcagg gcgtggtgat ggatcgcccg gaggcctggc ggttggcttc
     4021 cggtgtcagc ggattccagg cctttcaggt gtccgagaat cctccgccgc gggacatgga
     4081 acccgtgccc gtgagcatcc tcgccgctga ttggttactt gcggatacag gctccgcggt
     4141 cgtcatttgt aaccagccgg gagaacgatt ggcctgttat ctgccgccgg cctgtgtgat
     4201 tgtggccacc gcggataagc tccgggagca tcttcccgct gcttgggaag agattgttcc
     4261 cctagcacga cagccagaac gccgcggaga attcgtcatc attacaggac cgagtcggac
     4321 cgccgacatc gagaaaatct taatcttggg cgtgcacggt cccaaacgac tgatcgttta
     4381 tgtggttggt gagtaacccc cgccgggcga tccagcaagg ctggtcatgg ggccgcgttt
     4441 cggtggatga tgtcttctct cagcgagagc gccgcatgct cccattttta ccgttggcat
     4501 ggccattgcc atttccatta ccgttgcgga gcgaaaaccg cccggcgaga tagcaaatgg
     4561 cctgctgtcc cagcagtttc tcgatttgct gttctaggtc ggtgtcgagg gtaacgccca
     4621 tgccgtcaca ggccatgacg acgcggcgac catcgcgcaa ctgaagcatg agttgcaact
     4681 gacatgagcc tggatgatcg cgcacgattt ggtagaggtt ctggagtgtc acctcgttgt
     4741 gttgggcttc cgaaaatcgg atctggatcc cccgcgtaaa acgccgcttc aactccgcga
     4801 tgggaaccag ttcctggata atcaacgtcg cctcctcacc cccgccacgt ttatccacgg
     4861 tggccaaagc aacgagaacg gcgtcgtcgt ggaggtattc gccaaagcgg gtatacggtt
     4921 ccggccaaag aatacaacgg acgatccccg cggtatcttc caaatcaaac atggcaaact
     4981 tggtggggcc atcgttccgt ggtgcccgtg tatgtttggt aacaatggcc gacaccacac
     5041 cgccaacgac cacttcggtc ccatgggcga gttgtcctaa gtcggccgtg gtatgcgtgc
     5101 aataacggcc aagaagttct tcatattctc ggaggggatg acttgagaaa taaaagccga
     5161 gaacctcctt ctctttggcc agtctttcgt gatcaggcca ttctgggatg tcgggaagtt
     5221 gctgatcgac cacctggggg tttcttgtgc tgttgttacc aaagagactc agttgtccac
     5281 ggcgacgatc ttcggcagca cttgcccctg cttgaatggc gcgttccaaa accgccaata
     5341 gttgtgccct gtgaccgccc aatttatcaa aagccccggc cttgatcagc gattcgatag
     5401 ccgagcggtt aacgcttcct tgctcgagcc gttcgcaaaa ctcaaacaac gttcggaatg
     5461 gtccgtggcg acggcgctcc tccacaatgg cctcggcggc cgagccgcca caccccttga
     5521 tcgccgccag accgaaaacg atcttgccgt ccttaacggt gaattccact tcggacaggt
     5581 taatatcggg cggaatcacc tcgataccca tccggtggca atcctccaaa tgctcaacca
     5641 gcgagtcctt tcgggtgaaa ttgcggttga caatgtcgct tgacaacagt gccgccataa
     5701 actgcagcgg atagtgggct ttgaggtaag ccgtgatgta ggcaattcgg gcataggccg
     5761 tagaatggct cttgttgaag ccataaccgg caaacttctc gatgagcgtg aatagttctt
     5821 cggattcctt ttgggtgagg cctttttcgc aagcaccctt gacgaattgt tcccgatact
     5881 tggcaatttt atcgagcttc ttcttgctga tggccttgat gcacgtatag gcgtcggcca
     5941 gctcgatgcc gccaaggcga ttgagaatct gcatgacctg ctcttgatag accatcaccc
     6001 cgtgggtttc ttccagaatc tccttcatga cggggtgttt gtactcgggt ttcttgcggc
     6061 catgcttgac gtcgatgtag tcatcgacca ttccgccctc cagcggcccc ggccggtaaa
     6121 gggcattaac ggcgatgatg tcgcggatgt tatcgggctt catccgctgg agcagctccc
     6181 gaatgccacc gctttccaac tgaaagacgc ccttggtctc gccccggcag aggagttcgt
     6241 aggtcttggt atcatccagc ggaaacgaat acggatcgac cttttcgccc gtggtttgct
     6301 cgatcaagcc gatgaccttg gagaggatgg tcaggttccg caatcccaag aagtccatct
     6361 tcagcaaacc tgcccgctcc acgtcggcca tcgcccattg cgtgatcact tcttctttgc
     6421 cgggcacatg ctgaaggggg acgtattcaa ccagtggttt atcagcgatc accacgcccg
     6481 cggcatgggt gcccacattg cgggcgagac cctcaatctg cttggcgtag tcgatgacct
     6541 cgcgaacccg caagtctgtt tcgtaccgtt tgcgaaggtc ctcgcttttt tccagggctg
     6601 cctggatggt gaccttgggg ccttcaggga ccagggctgt gatttcattg acgaatgcca
     6661 caggatgtcc gagtacccgc cccacatcgc ggatcgccgc ccgtgccgcc atcgtaccaa
     6721 acgtgcagat ctgtgcgacg ttgttgtggc cgtatttttc cttcacatac tggagaactt
     6781 cctcacggcg ttcttggcag aagtcgatgt cgatatcggg cgcctcacgg cgattgagat
     6841 ccagaaaccg ttcaaagaga aggccgtatt caatggggca aacgtggctc aatttgagtg
     6901 cgtagctcac gagcgagccg acacccgacc cgcgggccgt ggcctgaatc cccttcgatc
     6961 gggcaaagcg aacgaaatcc caaacaatca gaaaataatt agcaaagcct agagtgttaa
     7021 tgacgtaaag ttctttttcc agccgctcca tgacctccgg cgccagttcg ccattgcgga
     7081 atcgccgcgg gtcgtgacgg tagcgttctt tgaggccctc aatacacaat tcccgcaggt
     7141 attgctcggc agtcttgccg tctggtgggg tgtaaattgg aaagtgacgg cgccccagtt
     7201 ccaattccaa atcaaccatg gacgcaatgt cttgcgtcct tcgcacagcc tcttccaagc
     7261 ccgaaaaaac ttggtacatc tgctccggcg agcgcaggta gtactctcgc ccatccatct
     7321 tcatccggtt ggtgtcgctg cggaatttgc cggtgttgat gcagagtagg acatcttggg
     7381 cgtcggcatc ctcgggcatg acgtaatgaa cgtcgcttgt ggcgaccagc gggatgttca
     7441 tcttcttggc cacggcgatg gcccctttca tcgccaaccg ctggatttcc gtgccattat
     7501 cttggatctc gatgaaatat cggtcaccga agaggctgcg gaaccaatcc gccacctcac
     7561 atgcccgtgc gatgtcgggg tcttccttgg caagcagggt tcgactcaat tctccagaag
     7621 gacaaccgct cagacagatg aggccctcgt gatgctgtgc caaaagctct ttgtcaatcc
     7681 gtgggcgata ataaaaacct tccagataag cggcggaagc aagtcgcagc aggttttgaa
     7741 aaccgcgttt atcctgagcc agcagtgtga ggtgataact ggcctccttc atgctagcgg
     7801 cttcgcgctg aaagcgactg cccggggcca cgtaggcttc gtatccgacg atcggcttga
     7861 tcccgcgatc cttggccttt tgataaaact cgagggcacc aaagaggtta ccatggtcgg
     7921 tcagcgccag ggccgtcatg caaagatcac gtgcccgatc caaaaggcga tcaacggttc
     7981 cagcaccatc gagcagactg taatgactat gacaatgaag gtgaacaaat ggcggcgtgg
     8041 ccatgctgat ttcctccctt gaaaagctcc tgagcatcat gcctcaggct tgtctaggcg
     8101 attgtagcac gactctcgcc ccctaaaaga gcgccctccg gggtggcaac ggtccccaaa
     8161 aagggcaagg gttgacaacc tgccgcgcag tggttcaaca tggcgattcc cggatcgcgc
     8221 ttggaaaaac cttaggtcgg tggttcgccg attacccgca aataatgggc cacgcgatca
     8281 taaagatcga gaaggccctg ccagtgtcgc tgctcgaggg tcacgctttc agaggcatga
     8341 tccatgctag ccgcctggac aaggcgtcgc aaccggatat gaaaatactg gagaagctcg
     8401 gtcaattggg cgagttgggc cggtgacaga tcctcgggca gatccggggg gacaagctca
     8461 tggatcgg
//
LOCUS       DAOSUT010000171         8384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4406_length_8384_cov_9.035298, whole genome shotgun sequence.
ACCESSION   DAOSUT010000171 DAOSUT010000000
VERSION     DAOSUT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_8384_cov_9.035298"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PLD05_13195"
     CDS             <1..1554
                     /locus_tag="PLD05_13195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOP78444.1"
                     /translation="RTLDHLSFSLSAWGPELVLCAGIVVLLLSRMVFPLGRYLPFVIS
                     LIVCCLALFLAGPWNGTWPIVGENSVPIFSGLLAWDGLGAFLKTLILGFSALFVVFLW
                     LSGFPKPAHATDFLVLYLGAVVGMCVMVSAEHLLLAFLGMEMASVPSYVLAGFDRRAR
                     QSAEAAMKFAVFGAASAGVMLYGISLLGGVLGTFSIPQMADQLMLLAASGDYHGQAVI
                     LVLSSLMILVGLAFKLSAVPFHFWLPDVFEGAAAEVGTFLSVVSKAAALGLLTRLVLG
                     LLGYGVLEATNAGVLPIVTFLVVTLSLLSAATCTYGNLAAYGQNNIKRLLGYSTIAHA
                     GYMLMPIAAAVAVLFRDASLARWAIATLLFYLVTYLFMNFAAFATVTFLRNAFRSETI
                     ADFAGLIRTSPGVVVCMSLALFSLVGLPPLAGFAGKFAIFSSLVFGGLWSLFVIAAIN
                     TVLSLFYYVRIIKTMVFDVPVRDPLPQVRLASPKGIFLAVLTVPVIVLGILWQPVFML
                     ACRAAGYPW"
     gene            1576..2037
                     /locus_tag="PLD05_13200"
     CDS             1576..2037
                     /locus_tag="PLD05_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78445.1"
                     /translation="MTRKHGYMLTPLAMLNTLLNRLSRSLACYICQARPFVSYRDENV
                     WQVIEEIASRHRELSHRLSEEIIRLKGVPDAGSFPPTYTRFNDLAVDYIAPKIAQELE
                     ETLTIIQKAKSEYHGHGELDNLLNQLADEVQAEIEALQRELHEAHPQECLK"
     gene            2083..2904
                     /locus_tag="PLD05_13205"
     CDS             2083..2904
                     /locus_tag="PLD05_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOP78446.1"
                     /translation="MWFDTHAHLYLLLDLAESGLPDRVTIERELSPILQKAKEEGVRG
                     IICPGIDRSTSELAITIADLFPSFVWAAVGIHPNSASQGTEEDWDVITQLATHPKVVA
                     IGETGLDWFRNYTPRDQQVTWFRRHIELSRHVNKPLIVHCRDAEDDMTAILHEAAVSG
                     KCIGVMHACAARWETVEQWLDFGMFVSFAGSVTFSNKKHLFVRETACHVPLERLLIET
                     DCPFLVPEPLRGRASVCLPSHIALTGTFLAKLRNVPVPDLAQVTSANAATLFQLG"
     gene            3053..>4776
                     /locus_tag="PLD05_13210"
     CDS             3053..>4776
                     /locus_tag="PLD05_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78447.1"
                     /translation="MSNEPDSPQIANRPKSSWQKRVPFQVDVAGIIRLMGQSLYSRSD
                     AAIRELLQNAHDGIMRRRAIDLQYRGRIDIRQHPADKVLEVSDDGIGLTPEEVEKYLG
                     TLGVGISGLLKRDLPNRGGQSLNLIGEFGIGLFSAFLLADRVEVITRHRQDGEAVRWE
                     AGPDSDICISSCDRSEPGTTVRLHLKPDQYPYATDEALLERAVKEYADFLPIPIHIND
                     RPQRVNIGSACWLEFTPDTHALELELESFFGETPLDLVVLRTEKPVTIRGALYVTPQR
                     TPGFTDAPTVTVTVQRMIISRRVYGLLPDWASFVRGVLELPDCSPTASREDLVRDATF
                     YAVREQLDTLLFNHFEGLARKNPSRWQSLIAWHRYLLAGAALEEPRLRDLLRDTYRFV
                     TSRGEMTFPEIVAASRTSTVYATDDDFVIWYNPNRYQEAWINEVFADFPAPCVHTLRS
                     FEETLLVLMVSDLQNEQISVDLRVAAPSSEDFAELVLGIQELSNVPHEWEQFLADLPA
                     LIRIGDYHGDIPVLAFLSEQAELQRTFEGIKKQGTMPCGFQRLVEAHFQYDKPKPNEV
                     ILNRRHPL"
     assembly_gap    4777..4876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5075..7882
                     /locus_tag="PLD05_13215"
     CDS             5075..7882
                     /locus_tag="PLD05_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78448.1"
                     /translation="MRSSDNKVLEELDQLLRRVHELTEKGYDRSAVQLAEEARRRARE
                     EHFVIPYLYANFLIMNSSQSLYDYRRGTDIAIETITLLESEERAREIQPDLPLDEYEK
                     TIYWLTSCAYDNLAEHYAIRYGYGGERVQGVAQDGFDVCHRTGKLECLACFRQYAMNV
                     YEAAGDLEMAMHFARGVFQSLPMVETNDRRAVGGMQYASQLTLMGRLKEALEVAEASV
                     GLAKVSYDPLKCRFLAQCCLGEILYLLGRGDEFEERCAVLGEPVDWTRLPPATENPEL
                     ARRKALFDSVRLTYEGALHQAEELLSFWDRDLYDRQALGPWFEVRLRRIALHRLMGYQ
                     DSVQKLGDTLRKEARQNQSWLYAERLAVMERGDVPLSPIPFLCAPQDTTFVAPGSDRV
                     RDEAAQQEMAAASGQESVSEKRSEQTQETAGSSETARGGFDWSSTPLAPKFDEWANRY
                     AQDQSETQVAAIFEEMVGIAPDTVQVPEDAARLLFVTGQMAELLGRELELWTWAKQFL
                     NIFPENGDVLNLVATAGLSAVQARSRRAAEMRALRGKRKKAAGRKKRNKRRGTLPTLQ
                     QLEEMFHKSLELSPRYPRHFLRAAQFYRFAGHHGEAEWCLARSFALDRSNPVAALALA
                     DMYSENDRDEDALAVLDLCLREGQVEDSEVAWNAGLTAFRLGRFPDAETYFNHAAQWG
                     CQEVWLFYYRAWTLLELKKVDEATEDILAFEAARGGSSLGSHTLWAWVAAHKGKIEDL
                     KKHVQEAMAIPLQQVNDLSDLGIEKNYHQMYRIARNALPADDVVLASLVDRLFQAGLV
                     PDELLEEERRKRPLLTNLRYYVCVLHQPLDDDWATHPACWENQKDWDAYTAYWGVLAH
                     NAEEAEHYAIQAQSRCYHLPPDVVEVELADEGFVDRPGVVFQGTRRYEDNSILSQLRD
                     YLDDSSDDSGGTGDFDDLDKD"
     gene            complement(8019..>8384)
                     /locus_tag="PLD05_13220"
     CDS             complement(8019..>8384)
                     /locus_tag="PLD05_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOP78449.1"
                     /translation="HGKPLKGSKVCILGMAYKKDVDDPRESPSFALMEMLLAGGAIVT
                     YNDPHIPKLPKMRHFRVPEMTSSELTAEFLASQDCVLIATDHSAYDYDFIVRHAPLVV
                     DTRNATKNVKIGREKIRKA"
BASE COUNT         1815 a         2102 c         2305 g         2062 t
ORIGIN      
        1 aggacattgg atcatctgag tttttcactc tcggcatggg ggccggagct tgttctatgc
       61 gccgggatcg tagtgttact actttctagg atggtgttcc cactggggcg ttacctgccc
      121 tttgttatct ccttgatcgt ttgttgccta gccctgtttc tcgccggtcc atggaatggc
      181 acatggccca tcgtggggga aaacagcgtt cccattttca gcgggttgtt ggcatgggat
      241 ggacttgggg cttttctgaa aacgctcatt ttgggcttct ccgcactgtt tgtcgtgttt
      301 ctttggttga gtggctttcc caagcctgcc cacgctacgg actttcttgt cttatacttg
      361 ggagccgtcg tgggaatgtg tgtgatggtt tcggccgaac acctcctctt ggccttttta
      421 ggcatggaaa tggctagtgt gccttcctat gtcctggcgg gttttgaccg acgtgcccgt
      481 caatcggcgg aggcggccat gaaatttgcc gtttttgggg cggcatcggc aggcgtgatg
      541 ctctatggaa taagcctcct cggtggtgtg ctgggcacgt tttccattcc acagatggcc
      601 gaccaattaa tgctccttgc ggcaagtggc gattaccatg ggcaggccgt tattttggtg
      661 ctcagtagtc tcatgatcct ggtgggactg gcattcaaat tgtccgccgt tccatttcat
      721 ttttggttac ccgacgtctt tgaaggagca gccgccgagg ttggaacgtt tctttctgtg
      781 gtatccaagg cggcagcgct ggggcttttg acacgactag ttctggggct tctcgggtac
      841 ggagtccttg aagcgacaaa tgccggcgtc ttaccgattg tgactttcct tgtggtcacg
      901 ctgtcgcttc tgtccgcagc aacctgcacg tacgggaatc tcgccgccta tgggcaaaat
      961 aatatcaaac ggcttctggg atactcaacg atcgcccacg cgggctacat gctgatgccg
     1021 attgcggcgg ccgttgcggt ccttttccga gatgcatctc tggcacgctg ggcgattgcc
     1081 acactgcttt tctacctggt cacttacctt ttcatgaact tcgcggcatt tgccacggta
     1141 acgtttctga gaaatgcctt tcgctctgag acaatcgcag actttgccgg attgattagg
     1201 acctctccgg gtgttgtcgt ctgcatgagc ctggccctat ttagccttgt ggggttgcca
     1261 ccacttgccg gctttgccgg aaagtttgcc atcttttctt cgttggtttt cggcgggctg
     1321 tggtcgcttt ttgtcattgc agccatcaat acagtgctca gcctttttta ttacgtgcgt
     1381 attattaaga cgatggtgtt tgacgtgccg gttcgcgatc ctctgccaca agttcgactg
     1441 gcctcaccga aagggatatt cctggcagtc ttgacggttc cggtaatagt gttgggcata
     1501 ctgtggcagc ccgtatttat gttggcgtgc cgcgctgcag gttatccttg gtaattgaat
     1561 cggtcgaatt gcattttgac acgaaaacac ggttatatgc ttactccctt ggcaatgctc
     1621 aatactctgt taaatcggct atcccggtcg ctagcctgct acatttgcca ggcacgacct
     1681 ttcgtctcgt accgggatga aaatgtttgg caagtgatcg aggaaatcgc gtcacgacac
     1741 cgcgaactgt cccatcgttt aagtgaagaa attatccgct taaaaggggt tcccgatgca
     1801 ggatcgttcc caccaactta tacccgattt aacgatctgg ccgttgacta catcgctcca
     1861 aaaattgcgc aagagcttga ggaaactctt acgatcatcc agaaggccaa gtccgagtat
     1921 cacggccacg gggaactcga caacttgctg aatcaattgg ccgatgaagt gcaggcggaa
     1981 atcgaggccc tccaacggga attgcatgaa gcccaccctc aagagtgcct taagtgatca
     2041 tgttttcgcc gattaaatga ttcttgcgta cagaaggcac ccatgtggtt cgacacgcac
     2101 gcccaccttt atctcctgct cgacctcgcc gaaagcggat tgcctgaccg tgtcaccatt
     2161 gaacgagaac tatcgccgat cttgcagaaa gcgaaagagg aaggcgtccg cggcatcatt
     2221 tgtccgggca tcgatcgctc aacgagtgaa ttggcaatca cgattgccga tctatttccg
     2281 agttttgttt gggcggctgt gggaattcat cctaactcgg cgtcgcaggg caccgaggaa
     2341 gattgggatg ttatcactca acttgcaacg catcctaagg ttgttgctat tggcgaaacg
     2401 ggcctggatt ggtttcgcaa ctacacgccc cgtgatcagc aggtgacatg gtttcgacga
     2461 catatcgaac tgagtcgcca tgtcaacaaa ccgcttatcg ttcattgtcg tgacgccgaa
     2521 gacgacatga cagcgattct tcatgaagcg gccgtttcag gaaagtgtat cggtgttatg
     2581 cacgcctgtg cggcacggtg ggaaaccgtt gaacagtggc tagattttgg gatgtttgtg
     2641 agcttcgcag gatcagtgac ctttagcaat aagaagcacc ttttcgtccg cgaaacagca
     2701 tgccatgtcc cgctggagcg gctattgatt gagactgatt gtccctttct tgtgcccgaa
     2761 ccattgcgag gaagggcttc ggtatgtctt ccatcacata tagccctcac agggaccttt
     2821 cttgctaagt tgcggaatgt cccagtccct gatctcgcac aagtaacgag cgccaatgca
     2881 gccacgctct ttcaactggg ttaagcggcc cacggttcat tgaccaaagt gccctaagcc
     2941 gtcataatca cgaaagtagc cgacgtcttg ccacgtaagg tcactaattc acattaacca
     3001 gcctgcttcc atctttgccg ttgccgagaa atggcgcaat taagttgtct caatgtcgaa
     3061 cgaaccagat tcccctcaaa tagccaatcg tcccaagagc tcgtggcaaa agcgagtccc
     3121 ttttcaagtc gatgtcgctg gaattatccg gctcatggga caatcccttt atagccggtc
     3181 ggatgcagca attcgtgagc tccttcaaaa tgctcatgat ggcatcatgc ggcggcgggc
     3241 gatcgattta caataccgag gccgtattga tattcgtcaa cacccagctg ataaggtact
     3301 cgaggttagt gatgacggga ttggactgac acctgaagaa gtggaaaaat acctaggaac
     3361 gctgggggtg ggaatcagtg ggcttctgaa gcgagatttg ccaaatcgtg gtggtcaatc
     3421 actcaatctg attggggaat tcgggatcgg tctttttagc gcatttctcc tagccgaccg
     3481 cgtggaagtg atcacccgcc accgtcagga tggtgaggcg gttcggtggg aggctggccc
     3541 ggattcggat atctgcatca gttcctgtga ccgttcagag ccgggaacaa cggttcggtt
     3601 acatctcaag cccgatcaat acccgtatgc cacggacgag gcgctcctcg aacgggcggt
     3661 caaggaatat gcggatttct tgcctatccc gattcacatt aacgatcgac cgcaaagggt
     3721 caacattgga tccgcatgtt ggctagagtt cacaccggac acccacgcct tggaattgga
     3781 actggaatcc ttcttcgggg aaacgccttt ggatttggtc gttttacgga cagagaagcc
     3841 ggttaccatc cgcggcgctc tttacgtgac gccccaacga acgccaggtt tcacggatgc
     3901 ccccacggtt acggtcacag tgcagcggat gattatctcc cgaagggtct atggtttgct
     3961 tccagattgg gcgagctttg ttcgcggtgt ccttgaactt cccgactgtt cacctacagc
     4021 tagccgtgag gatctggttc gcgatgccac attttacgcc gtacgggagc aattggatac
     4081 gcttctattt aatcactttg aggggttagc ccgaaaaaac ccaagtcgtt ggcaatcgct
     4141 gatcgcctgg catcgttatt tacttgcggg ggccgctctg gaggagcctc gccttcgcga
     4201 ccttttgcgg gacacctatc gttttgtgac ctctcggggc gagatgacct ttcccgagat
     4261 tgtcgcggca agccgaactt cgacggtcta tgcaacggat gatgatttcg tcatttggta
     4321 caatcctaat cgttaccagg aagcttggat caacgaggtc tttgccgatt ttccggcgcc
     4381 ttgtgtccat accctgagaa gctttgagga aacacttttg gtgctcatgg tctcggacct
     4441 acagaatgaa caaatctcgg ttgacctgcg tgtggcggcc ccatcaagcg aagatttcgc
     4501 cgaactggtc ctgggaatcc aggagctatc gaacgttcct catgagtggg aacagtttct
     4561 cgcggatttg ccagcattga ttcgaattgg ggactatcat ggagacattc ccgtgctggc
     4621 gtttttgagt gaacaagccg agcttcagcg aacatttgag gggatcaaaa aacagggaac
     4681 catgccgtgt ggctttcagc gcctcgtgga ggcccatttc cagtacgaca aaccgaagcc
     4741 caacgaggtc attttgaacc gccgtcatcc gctaatnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnngaat cgtttcagtt ggatcggaat ctcgataaat tgaacgcacg
     4921 gtaaagaatc gctgttacgg agcgagaacg acgtcaattg gttctttcgg cgaacctcga
     4981 ggttttcttg ggggataact tgggtgataa ggagtcggca cgcctggctg attaggcaag
     5041 gtggcagccg agtgtcaaaa taaggaacga gataatgcgt tcttccgaca acaaggttct
     5101 agaagagctg gaccagttat tacggcgggt ccatgagttg acagaaaagg ggtacgatcg
     5161 ctccgccgta caattggccg aagaagcccg acggcgagct cgcgaggagc attttgtcat
     5221 accttatctt tacgccaatt tcttgatcat gaacagttca caaagtctgt acgactatcg
     5281 ccggggcacc gatattgcca tcgaaaccat cacgcttctc gaaagcgaag agagagctcg
     5341 ggaaatccaa ccagacctgc cgctggatga atacgagaaa acgatttact ggctgacgag
     5401 ttgcgcttac gacaacttgg ccgagcatta tgcgattcgc tatgggtacg gcggagagcg
     5461 ggtccagggg gttgctcagg acgggtttga tgtgtgtcat cgcacgggca agctggagtg
     5521 tctcgcgtgc tttcgccaat atgcgatgaa tgtctatgag gccgctggtg atttggaaat
     5581 ggccatgcac ttcgcgcgag gggttttcca gtcgcttcct atggttgaga cgaatgaccg
     5641 gcgggcagtg ggagggatgc aatatgccag tcagttgacg ctcatgggcc ggttgaaaga
     5701 ggccctggag gttgccgagg cgtccgttgg gttggccaaa gtcagttatg atcccctgaa
     5761 atgtcggttt ctcgctcagt gttgtttggg cgagattcta tatctgctgg gacgtggcga
     5821 tgaatttgag gagcgctgcg cagtcttggg agaacccgtg gattggaccc gtttaccccc
     5881 agctaccgag aaccctgagc tggctcgtcg gaaggcgctc ttcgatagcg tccgtctgac
     5941 ctacgagggg gcactccatc aagcagaaga actcctttcc ttctgggatc gtgaccttta
     6001 tgaccgccaa gccctcggac catggtttga ggtccgtctc cggcggattg ccctgcatag
     6061 acttatgggc tatcaagact cggttcaaaa attaggcgat accttgcgga aagaagcacg
     6121 ccaaaaccaa tcctggctgt acgcggagcg actggccgtg atggaaaggg gagacgttcc
     6181 cctttcaccc ataccatttc tctgtgctcc gcaagacact acttttgtgg cgccaggatc
     6241 agatcgggta cgcgacgaag ccgcgcagca agaaatggcc gcagcatctg ggcaagaatc
     6301 ggtttctgaa aaacggtcag aacaaactca ggagacggcg ggttccagtg aaacggctcg
     6361 gggtggcttt gattggtctt ctacgccgtt ggcaccaaaa ttcgatgaat gggcaaatcg
     6421 gtatgcccaa gatcagtcgg aaacacaagt ggccgctatt ttcgaggaga tggtcggcat
     6481 tgctcccgac acagtgcaag tgccagaaga tgcggcacgg ctacttttcg tgacaggcca
     6541 gatggccgaa cttctcggcc gggaactgga gttgtggact tgggcgaaac aattcctcaa
     6601 catttttccg gaaaatggag atgtcctcaa tttggtggct acggccgggc tttcggcggt
     6661 tcaggcacgt tcccgaaggg cggcagaaat gcgggcgttg cggggaaagc gaaaaaaggc
     6721 ggcaggccgg aaaaaacgca acaaacgtcg aggaaccctc ccgacacttc agcaactgga
     6781 agaaatgttc cacaaatcct tggagctgtc gccgaggtat ccacgccact ttctccgggc
     6841 agcgcagttt tatcgcttcg ccggacacca cggggaggcg gaatggtgtc tcgcccgatc
     6901 gttcgccttg gatcgcagca atcctgtggc ggcattggcc ctggccgata tgtactcgga
     6961 aaatgaccgc gacgaagatg ccttggccgt cctggacctg tgcctccgcg aaggtcaggt
     7021 ggaggattcc gaagttgcct ggaatgccgg cttgacagcc tttcgactag gccggtttcc
     7081 agatgcagaa acctatttca atcacgccgc ccaatgggga tgccaagaag tttggctctt
     7141 ctattatcgg gcatggacac ttttggaact gaaaaaggtc gatgaggcca cggaggatat
     7201 tctggccttt gaagctgccc gtggcggttc tagcctgggt agccacacgc tttgggcttg
     7261 ggttgccgct cataagggga agattgagga ccttaagaaa catgtgcaag aagcgatggc
     7321 gatcccactt caacaggtca atgatctttc tgatctcggg atagaaaaga actatcatca
     7381 gatgtaccgc attgcgcgga atgccttgcc tgccgatgat gtcgtcttag ccagtctggt
     7441 tgatcggctc ttccaggcgg gattggttcc ggacgagctt ctcgaggaag aacgccgaaa
     7501 aaggccactt ttaaccaatc tacggtacta cgtctgtgtt ctccaccaac cattagatga
     7561 cgactgggca acgcatcctg cttgctggga aaatcaaaag gactgggatg cctacacggc
     7621 atattggggc gttttagccc ataatgcgga agaggcggaa cactatgcca ttcaggccca
     7681 gtcgcggtgt taccatttgc cacctgatgt tgttgaggtc gagcttgcgg atgaagggtt
     7741 cgttgaccgt cctggcgttg tgtttcaggg aactcggcga tacgaagata atagtatcct
     7801 ttctcaatta cgtgactatc tcgatgattc gtcggatgat tccggcggta ccggcgactt
     7861 cgatgacctc gacaaggatt agcgctaggg aatcccaggc aaactgaccg cggaggttgg
     7921 ccccagcaat cctagggagc tcgcgcggcc aacgtagctc gcagaggcaa ggggcaggta
     7981 tattcgagaa gcttctcaag cttccggctt aaacccgctt atgccttgcg aatcttttcg
     8041 cgtccgatct tgacgttttt cgtggcattt cgagtatcaa cgacgagcgg cgcgtgtcgc
     8101 acgatgaagt cgtaatcata ggcagagtga tccgtggcaa taagaacgca atcctgagaa
     8161 gccaggaatt ctgccgttaa ctcggaactt gtcatttctg gcacccggaa atgccgcatt
     8221 ttcggaagct tgggaatatg gggatcgttg taagtcacaa tggccccgcc cgcgagaagc
     8281 atctccatga gggcaaatga aggactttcc cgcggatcat ccacatcctt cttgtaggcc
     8341 atccccagaa tgcacacctt gctccctttc aacggcttgc catg
//
LOCUS       DAOSUT010000172         8313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4437_length_8313_cov_9.878179, whole genome shotgun sequence.
ACCESSION   DAOSUT010000172 DAOSUT010000000
VERSION     DAOSUT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_8313_cov_9.878179"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLD05_13225"
     CDS             complement(<1..1226)
                     /locus_tag="PLD05_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78450.1"
                     /translation="MRLQRTTNRHWSRREFLVTAGAVLGTTLTLASRAAGVHVAEDNT
                     IRLALIGCGGRGTGAVADAFSTTGGPVKLYAMADLFQDRLNASYQNLKTKYADRVDVP
                     PERQFVGFDAFRHAIDCLRPGDVAILATHAVFRPMHFEYAVKKGVNVFMEKSFAVDAP
                     ATRRLMKAAEESEAKNLKVGVGFMWRHSRARQEAIKRIHDGAIGDVHTLRIYRVHGPV
                     YTPNKPPEANEIEFQIRHAVCFNWVSGGFFVDWHCHNVDIACWAKGSWPVEAQAMGGR
                     CYVDAGNLFDHYCVEYTFADGAKLFAFSRHMANCWSTYADYVHGSKGSAVLMTSLADP
                     QPKLYKSQKMTADQVIWEFPAPDPNPYHAEWQVLLDAIRQDQPHNEARRAAEANMAAL
                     MGRMAAHTGQYVTWEQA"
     gene            1492..2412
                     /locus_tag="PLD05_13230"
     CDS             1492..2412
                     /locus_tag="PLD05_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP78451.1"
                     /translation="MAYRGLNRELSRRNVVRGIAALSAGTLANSVSGAISWASQERTN
                     QPRWRLRLSASSINFSSLPIEEACKRIASLGFEAIDIWSAHAGCPHLDDVQRRLGADG
                     LQQVLKACGLKLYAFSVYSGGYLRYADLLGQCGGGVAVRGSAAPCEPKDLVPRTKAFL
                     ESLKQELEKAEASNSYLAIENHGNALLDSCDSLKAFVDLNKHSRLGIALAPYHIQARG
                     ESVETAIRICGGQLLYFYAWQHGSGLKQLPGYGETDCAPWLKALAEVSYTWYVNPFMH
                     HEPPPNEMAAALANSCNYLRQCAKDAGLEG"
     gene            2721..3956
                     /locus_tag="PLD05_13235"
     CDS             2721..3956
                     /locus_tag="PLD05_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78452.1"
                     /translation="MTPKSWWNRTILGAGITSAFGDLCYEMGNVVLPGLLSALGIPAA
                     LLGVMEGVADGLASFTKLVAGYIGDRFGHRKTLVVAGYALNTIGQGFMAIATGFWLIA
                     LGRILAWFGRGIRGPLRDEIMTAAVPPEQRGRAFGFHRAADTFGAILGPAIGTGLVAW
                     LGSSLATVSPTMPFRSVLVISMIPGALAVLSFAVLVHDPGGGGKPKLRFWGAMRSMPS
                     SFRHYLVAVGIYGSGDFAPTLLILAATQTLSPSYGVTMAGALAGSLYVLRNTVQTLVS
                     YPVGWLSDRLGARRALVGGYVLGTLSCVLLAIIFALGYWNILPLAAVFILSGICAAVQ
                     ETVEPVVTAALVPAEIRATCYGVLGAVNGVGDLISSFFVGLIWTLVAPQWAFLAAGGT
                     LLMGTALLAALKNHGADSA"
     gene            complement(3989..>4875)
                     /locus_tag="PLD05_13240"
     CDS             complement(3989..>4875)
                     /locus_tag="PLD05_13240"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HOP78453.1"
                     /translation="GLDVLALSDHIEYQPHKADVPTNHNRPFEVALGRARELGIILIR
                     GAEITRDTPPGHFNVIFLKDINPLVKDDLVEAIAAGCEQGGFVFWNHHAWKGEELGRW
                     LDIHSTLYEKKLLHGMEVANGDDYYPTAHRWCLEKGLTMVGNSDIHGPDLLQKNTSAE
                     HRTMTLVFVAEKSEQGVLEALRAGRTAVWCGPLMIGKPEWLTALAEASLEIDPPHMRS
                     KTSVWVKVRNKCAMDLVLKRTGQFGPAEVTLKANTTAIFRINVPDASQPIPLEYEITN
                     FLAAPGEGLDVSYTIPAP"
     assembly_gap    4876..4975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5147..6250
                     /locus_tag="PLD05_13245"
     CDS             5147..6250
                     /locus_tag="PLD05_13245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOP78454.1"
                     /translation="MRLFLMALIFGTGAVMCNLSFGQVIFPGERWEEVSPASVGLDKG
                     RLDEAAMILSTKVGRDGAKRLVIVRHGRIVWKGSEADERQGVWSMTKSFTSTVAGLLI
                     AKGRCTLDTKVADVLPELKTAYPEVRLRHLLTMTSGYRAMGDEPRGDYLHGPSVTPFV
                     PNPEPLFTPPGTQYAYWDSAMNLLGLVLTKLAGAPLKEVFRRLVAEQIGMSNWDWGDF
                     GEIDGIVVNGGAGNMNRHVKISACDMARFGWLMLNRGRWRDKQIVPEWWIQEATRVQV
                     PATLPWAQPQSQIDGRGVYGFNWWVNGVGANGERALPAAPVGTFWASGHNNNRCFVIP
                     EWDMVIVRLGLDGRAPAESWNEFLAKIGEAIRE"
     gene            complement(6352..>8313)
                     /locus_tag="PLD05_13250"
     CDS             complement(6352..>8313)
                     /locus_tag="PLD05_13250"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOP78455.1"
                     /translation="NFLLFGQPDVTIVVVDATRLERNLNLALQVLEITHRVILCVNLI
                     DEAERHGLVIDTELLAAELGIPVVATSARFGRGIDTLLKAIEGVATGEIQCHPLRVGG
                     STRQLSEAVQDLTKELERAFPHLTNLGWIAIRLLDGDERIVKAIRTGELAQLHRQNHE
                     AVSRDSATENRPPSLDEGPSEDSRERILAKASAWRWQIGPNAHQTLVEAIYAHAGRIA
                     DRAVRRKEGVSSTWDEKLDSILTSPIWGYPTMIALFVVVIWITVQGANYPSQVLSWFL
                     VETLHPLLRSAAVGVHLPNWLIGLFLDGGYLATAWVISVMLPPMAIFFPLFTLLEDLG
                     YLPRVAFNLDRLFRWAGGHGKQALTMSMGFGCNAAGVVACRIIDSPRERLVAILTNNF
                     SLCNGRWPTQILLATVFLGSLVPSYLAGFVAAGGVIAVALLGVVMALVTSRLLTQTVL
                     KGEPSTFHLELPPYRPPQILQTLYTSLVTRTLVVLWRAVVFAFPAGLVIWLIANVHIG
                     SQPLAEYLVRFLDPIGLAVGLNGIILLAYVVAIPANEIVIPTVLMLTALLGDVTLAGA
                     QAGVLFEPEDLTATGEVLRQAGWTPITAICLMLFSLMHNPCSTTIYTIYAETKSVKWT
                     VVAVVMPLILGFSACFLVNQIARYCLGLG"
BASE COUNT         1787 a         2235 c         2293 g         1898 t
ORIGIN      
        1 gcctgttccc atgtgacata ctggccggtg tgagcggcca tccggcccat gagggctgcc
       61 atgttggctt cggcggcgcg tcgggcctcg ttatgcggct gatcttggcg aatggcatcc
      121 agcaacactt gccactcggc atgataagga ttgggatcgg gcgccgggaa ctcccagatc
      181 acttgatctg ccgtcatttt ctgactcttg tagagcttcg gctggggatc cgccagcgat
      241 gtcatgagaa cggctgagcc tttacttcca tgaacgtagt cggcatacgt ggaccagcag
      301 ttggccatgt gacgggagaa tgcgaacagt tttgcaccat cggcaaaggt gtattccaca
      361 cagtagtgat caaagagatt ccccgcatct acgtaacaac gccctcccat ggcttgggcc
      421 tctacgggcc aagacccttt ggcccagcac gcaatatcca cgttgtgaca gtgccaatcg
      481 acaaagaagc cgccactgac ccagttgaag cagacggcat gacggatttg gaactcaatt
      541 tcattggcct cggggggttt atttggcgtg tagaccgggc catgcacccg gtagatacgt
      601 agtgtgtgta catctccaat ggcaccgtcg tggatccgct tgattgcctc ttgcctagcc
      661 cgcgaatgac gccacatgaa gccgacccca actttcaggt tttttgcctc cgactcctct
      721 gcggccttca tcaacctccg tgtggcagga gcatcaacgg cgaaagactt ttccatgaaa
      781 acgttgacgc cctttttcac ggcgtactca aaatgcatcg gccgaaacac cgcatgagtc
      841 gccagaattg ccacatcgcc tggacggagg cagtcgatag cgtgccgaaa tgcatcaaat
      901 ccaacgaatt gtcgctcggg cggtacgtcc acgcgatcgg catactttgt tttcaagttt
      961 tgatagcttg cattcaggcg gtcctggaaa aggtcggcca tcgcatacaa tttgaccgga
     1021 ccacccgttg tggagaatgc atcggccacg gcaccagtcc cgcgtccacc gcatccgatg
     1081 agggcgagcc gaatggtgtt atcctctgcc acatggacac cagcagctcg ggatgctagg
     1141 gtcaaggttg ttcccaaaac agcccccgcg gtaaccaaga attcccggcg agaccaatga
     1201 cgattcgttg ttcgctgcaa acgcatgacc tcatctcctc tttcttacaa tcctctttct
     1261 aacaagtcgc gcacggccct gtcaggtgta acaacatgtt ccagtcggtg tcagcccttc
     1321 gttgagctgt gcttatcata ccgaagagct atggcgatta ctacaacgga attacaatga
     1381 attgcgactt ggcaagggcc aagaaaggat gatcatcata actggattgg acctctcaaa
     1441 ctgagttggg tatctatgca gttcaccgaa tctagcaatt gggattgtga catggcctac
     1501 aggggattaa atcgagagct ttcgcgtcgc aatgtcgtcc ggggaattgc tgcactttcc
     1561 gccgggacgc tggctaacag cgtttctggg gcgatatctt gggcttctca ggagcgaacg
     1621 aatcaaccga gatggcgact acgactgtcc gcatcgtcta tcaatttctc ctccctcccc
     1681 atcgaggagg catgtaaacg gattgccagc ttgggatttg aggctatcga tatttggtcc
     1741 gcccacgctg gttgtcctca tctggacgat gtccagcgcc gcttgggggc cgatggtttg
     1801 cagcaggtcc tgaaagcatg tgggctgaag ctttatgcct tttcggttta cagcggcggt
     1861 tacttgcgct atgccgactt actgggacaa tgcggtggcg gggtcgccgt tcggggcagt
     1921 gcagcacctt gtgagccgaa agacctggtc cccagaacga aggcctttct ggaatccctc
     1981 aagcaagagt tggaaaaggc ggaggcgtcg aactcctatc tggccattga gaaccatggg
     2041 aatgccctct tggattcttg cgactctctc aaggcctttg tcgatctgaa taagcattcg
     2101 cgattgggga ttgctttggc gccgtaccac attcaagcac gtggggaatc tgtggagacg
     2161 gccatccgca tctgtggagg acaacttttg tacttttacg cttggcaaca tggaagtggg
     2221 ctcaagcagc ttcccggtta tggcgagaca gactgcgctc cctggctaaa agcccttgcc
     2281 gaggtctcct acacatggta cgttaatccc ttcatgcatc atgagccgcc gcccaatgaa
     2341 atggcagccg ctctggcgaa ctcatgcaac tacctgcgcc agtgtgcgaa agacgctggt
     2401 ctggaaggat agtaagcgcc accgcttttg cgttaaagag tctagcccaa aaaatccccc
     2461 ctctccctcc gggagagggt tggggtgagg gtaaccgatc gtcggagttg atcgagagaa
     2521 ccggcagcga ttcgagccac cctcattccg ccccctttcc cgccggacgg gtgagggaag
     2581 attgtgggat aggcttcaag tggggtgatt aatgagttaa cctaccttgc cttcttgtag
     2641 cattccgtgc ttgccaactc ggtgaattct gtatagcaac acaataggcg aatacgatcc
     2701 tgtttcaagt tttcaaaatc atgaccccga aaagttggtg gaatcgaaca atcctcggtg
     2761 cgggcataac cagtgccttt ggggatctgt gttatgaaat gggcaatgtg gtgttgcccg
     2821 gactcctatc tgccctggga attcccgccg ctttactcgg agtgatggaa ggcgtggccg
     2881 acggtctcgc cagttttaca aaactggtcg ccgggtatat cggcgatcgg tttgggcatc
     2941 gtaagacgct tgttgtggca ggttatgccc tcaataccat tggtcaagga ttcatggcca
     3001 ttgccaccgg gttttggctt attgcccttg gacgcatcct tgcatggttc ggacggggta
     3061 ttcgtgggcc gctccgcgac gagatcatga cggcggcagt gccgccggaa cagcgcggtc
     3121 gggccttcgg ttttcaccgc gcagccgaca catttggagc gattctgggg ccagctatcg
     3181 ggaccggctt agtggcttgg cttggcagct cattggctac agtgtctcca acaatgccgt
     3241 ttcgctcggt cttggtgatt agcatgatcc cgggggccct tgcggttttg agtttcgcgg
     3301 ttcttgttca tgatcccggg ggaggtggaa aaccaaaact gcgtttttgg ggtgctatgc
     3361 gctcgatgcc gtcttccttc cgccactatc ttgtagcagt gggaatctac ggaagtggtg
     3421 atttcgctcc cacacttctc atcctggcag ccactcaaac actgtcgcca tcatacggcg
     3481 ttacgatggc gggggccttg gcgggctcat tatacgtact gcgcaacaca gttcaaacgt
     3541 tggtttccta ccccgtcggc tggctctccg atcgcctcgg agcacgccgc gccttggttg
     3601 gggggtatgt cctgggcaca ctgtcctgcg tgctccttgc gatcatcttt gccctcggtt
     3661 attggaacat tctacctttg gcggcagtgt tcatcctctc gggaatctgt gccgcagtcc
     3721 aagaaacggt cgaacccgtt gtcacggctg ctctcgtccc cgctgaaatc cgcgcgacgt
     3781 gctatggagt gctcggcgcg gtcaatggcg tgggcgatct tatctcaagc tttttcgtcg
     3841 ggctcatttg gacgctcgtt gccccccaat gggctttttt ggccgcaggc ggtacccttc
     3901 tgatggggac ggcgcttctc gcggctttaa aaaaccacgg agcggattcc gcatgagccc
     3961 gcttaaccga gtttaactgc ccagcggatc acggggctgg gatcgtgtaa ctaacgtcca
     4021 gtccctcgcc aggagcagcg aggaaattgg tgatctcgta ttccaacggg atgggttggg
     4081 aggcgtcagg gacattgatc ctaaagatcg ccgttgtgtt ggccttcaac gtgacctctg
     4141 ccgggccaaa ttgacctgtc ctttttagaa cgaggtccat ggcgcacttg ttgcgaacct
     4201 ttacccacac gcttgttttg cttcgcatat gcgggggatc aatttccaga gaggcctcgg
     4261 ccaaggcggt gagccattct ggtttcccga tcatcagagg tccacaccaa acggctgttc
     4321 gcccggcacg aagggcctcc aacaccccct gttcgctctt ctcggccaca aacaccagtg
     4381 tcatcgtccg atgctccgcc gaggtgtttt tctggagtag gtccggaccg tgaatgtcgg
     4441 aattgcccac catcgtcaac cctttttcca ggcaccaacg gtgcgcagtt ggatagtagt
     4501 cgtctccgtt ggccacctcc atcccgtgaa ggagcttctt ctcgtatagt gtggagtgga
     4561 tgtccagcca acgtccgagt tcttccccct tccacgcatg gtgattccaa aagacgaatc
     4621 ccccttgctc acatccggcg gcaatggcct ccaccaaatc gtccttcaca agcgggttga
     4681 tatccttgag gaaaatcacg ttgaaatgtc ccggcggagt gtcccgagtg atttcggcac
     4741 cgcgaataag aataatcccc agttcccggg cacggcccaa ggcgacctcg aatggccgat
     4801 tgtgattggt cggcacatcg gccttgtgag gttggtactc gatgtggtca ctcagagcca
     4861 agacatccag cccgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgagg
     4981 aaatggcaag gcatgatggt ggcagcgatc ctgtgagtag gtcctcgcaa agccacctgc
     5041 acaaagtaat aatgattccg ttggttttta gacggctaca gtgtggtgtt ccgaagccat
     5101 ccatatgtcc gcggattgat atttcttaaa agcggaggac aaggccgtga gactgttttt
     5161 aatggcactc atttttggga ccggtgcggt gatgtgtaat ctgagttttg gacaagtgat
     5221 ttttccggga gagcggtggg aagaggtaag ccccgcgtcc gtgggcctcg acaaaggacg
     5281 tctcgacgag gcggcgatga tcctctctac aaaagttggc agggatgggg ccaagcgatt
     5341 ggtgattgtc cgtcatgggc gaatagtttg gaagggttct gaggccgatg agcgtcaggg
     5401 tgtctggtcc atgacaaaat cgtttaccag cactgtggcg ggtttgctta ttgccaaggg
     5461 acgctgcacc cttgatacaa aggttgctga tgtactaccg gagttgaaaa cggcctatcc
     5521 cgaggtgaga ctccgtcatc tcttgactat gacaagtggt taccgtgcga tgggcgacga
     5581 gccccgtggc gattacctac atggaccgag cgttacaccc tttgttccca acccagagcc
     5641 cctctttacc ccgccgggaa cacaatacgc ctactgggat tccgccatga atctcctcgg
     5701 tttggttctc acgaagttgg ctggggcacc actaaaggaa gtctttcggc gtctggtagc
     5761 cgagcaaatc ggcatgagca attgggactg gggtgatttt ggggagatcg atggaatcgt
     5821 tgtcaacggc ggcgccggca acatgaaccg gcacgtgaag atttcggcct gcgacatggc
     5881 ccgctttggt tggctcatgc ttaatcgggg acgctggcgg gacaaacaga tcgtccccga
     5941 gtggtggatt caggaggcca cccgtgttca agttcccgct accctgcctt gggcccaacc
     6001 ccagagtcag atcgacggaa gaggcgtcta tggattcaac tggtgggtca atggagtggg
     6061 ggccaatggt gagagagcac tgccggccgc accggtgggt accttttggg cctctggtca
     6121 caacaacaat cgctgctttg tcataccgga gtgggacatg gtaatcgtcc gcctcggttt
     6181 ggatggcagg gctccagccg aaagttggaa cgagtttctc gcaaagatcg gcgaggcaat
     6241 ccgagaatag tcgcgcgatc gctcaacctg gttttcgttt tcggttgctc gattgttagt
     6301 tgttggcgct ataggcggac acggttgtcg ctaatatcca tccgggttcc ttcatcccaa
     6361 acccaggcag tacctcgcaa tctgattgac gagaaagcat gccgagaacc caagtatcaa
     6421 cggcatgact acggccacga ctgtccattt aacgcttttt gtctctgcat aaatggtgta
     6481 aatcgtggtg gaacatggat tgtgcatcaa gctgaacagc ataagacaga tggccgtgat
     6541 tggtgtccac cccgcttgcc gaaggacctc gccggtcgcc gtcaagtcct ccggttcaaa
     6601 gagcacgccg gcttgagcgc ccgctagggt tacatcgcca agcagtgccg tcaacatcaa
     6661 aacggtcggt atcacaatct cgttggcagg aatagcaact acgtaggcca gcaagataat
     6721 tccattgagg cccactgcca acccgatggg atcaaggaaa cgaacaagat actcagccag
     6781 gggctgcgac ccaatatgaa cattggcgat gagccaaatg accaggcctg ccgggaaggc
     6841 gaacacgacg gctcgccaca gcacgaccaa tgtccgggtg accaaagagg tatagagagt
     6901 ctgtaagatt tgcgggggac ggtagggcgg caactccaag tggaatgttg atggttcccc
     6961 cttcaagacc gtttgagtga gaagtcgcga tgtaacaagt gccatcacaa cgcccagcaa
     7021 ggcgacggca ataacacctc cggccgccac aaagccggct aggtaagacg gtacgaggct
     7081 tcctaaaaaa accgtggcaa ggagaatctg tgttggccat cgaccattgc acagtgaaaa
     7141 gttgttcgtc aggattgcca ccaggcgttc ccgtggacta tcaatgattc gacaggcaac
     7201 gactcctgcc gcattacagc cgaaacccat agacattgtt aaagcctgct tgccatgacc
     7261 gcccgcccag cggaagagac gatccaagtt gaatgccacc cggggaaggt acccgaggtc
     7321 ctcgagcaac gtaaacaaag ggaaaaagat ggccattggc ggcagcatca cactgatgac
     7381 ccatgccgtt gccaggtacc ccccatcaag gaataggcca atcagccagt ttggcaaatg
     7441 aacaccgacg gcagccgatc gcagtaaggg atggagggtt tccaccaaaa accagctcag
     7501 cacttgagac ggataattgg ctccctgcac agtgatccaa atgaccacga caaatagggc
     7561 aatcatcgtc ggataacccc agatggggct tgtcaggatg ctatccagtt tctcgtccca
     7621 agtcgaagag acaccttcct tccttcggac agcccggtct gcgatccgac ccgcatgagc
     7681 gtaaatagct tcaaccaatg tttgatgagc attaggacca atttgccacc gccacgctga
     7741 ggcctttgca agaatccgtt cccgcgagtc ttcggatggt ccttcatcca gtgaaggtgg
     7801 tcgattctcg gtggcagagt ctcggctcac tgcttcgtga ttttgccgat gcaattgggc
     7861 cagttctccc gtccgaatgg ccttgacgat gcgctcgtcg ccatccagca gccttatcgc
     7921 gatccagccg aggtttgtca aatgggggaa agcccgctca agttcttttg tcagatcctg
     7981 gacggcctcg cttagttgac gtgtactgcc ccccacccgc aacgggtgac attggatctc
     8041 ccccgtggct acaccctcaa tcgctttcaa aagcgtatcg atgccgcgtc caaatcgtgc
     8101 tgaagtggca acgacgggaa tacccaattc tgcagcaagc agctcggtgt caatgaccag
     8161 tccgtgacgc tcggcctcat cgatcaaatt gacacagaga ataacgcggt gtgtgatttc
     8221 aaggacttgc agggctagat tgagattcct ttccaatctt gttgcgtcca ctacaacgat
     8281 cgtgacgtcg ggttgtccaa aaagtaaaaa gtt
//
LOCUS       DAOSUT010000173         8144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4527_length_8144_cov_9.637409, whole genome shotgun sequence.
ACCESSION   DAOSUT010000173 DAOSUT010000000
VERSION     DAOSUT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_8144_cov_9.637409"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLD05_13255"
     CDS             <1..839
                     /locus_tag="PLD05_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOP78456.1"
                     /translation="FLPIMPGLPQSSSVQERRQQLLELIRTRRFATLEELAAALGVSE
                     STVRRDLEALEQQGDARRIHGGALYTGTSPKLAHFDVRQPMRWEQKRAIARTAVELVS
                     AGDTVILDGGTTTYEVARLLLGVPIHVVTNSLPVANLFASDPASDLVFVGGSICPRTG
                     VAQGPHAEAMIAGLRVRKAILSVAAANDEGFFNNNVLLVETERAMMQAADEVIIVADS
                     SKFGHRSLMHLCPLDEVDCIVTDEGLSDEWRAKITSLGVRLIVAPLAEEAARGGTTNP
                     PD"
     gene            931..1596
                     /locus_tag="PLD05_13260"
     CDS             931..1596
                     /locus_tag="PLD05_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOP78457.1"
                     /translation="MSVAAPIDRGQVEKIVREVVIKLLGGPAERPEVVVSVSARHCHL
                     SREHIDILFGPGYELKPYKWLYQEGFYAAEETVMIVGPRRRMLPTLRVLGPPRSKTQV
                     ELAFTDAISLGIDAPVRLSGDLKDTPGCVLIGPEGSVDLKEGVIRAARHVHMNVRDAA
                     FYGVKNGDVMSLRVESGPCTTVFEGLVVRADEQSKLEVHLDTDEGNAADIDHATRVEL
                     FKP"
     gene            1705..1998
                     /locus_tag="PLD05_13265"
     CDS             1705..1998
                     /locus_tag="PLD05_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOP78458.1"
                     /translation="MEALGMLETKGFVTLVEATDAMLKAANVQFLGWDKVGSGLVSVF
                     VTGDVAAVKAATDAGAAAASRIGEVVSVQVIPRPHDDLNVVLPTAPAKGGKTD"
     gene            2071..2340
                     /locus_tag="PLD05_13270"
     CDS             2071..2340
                     /locus_tag="PLD05_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOP78459.1"
                     /translation="MQDAIGLIETRGLVALVQATDAMAKAANVQIVKRVQIGGGLVTT
                     IVRGDVGSVRAAVEAGANAAAQVGELVASHIIPRPAEGVIAAYLT"
     gene            2523..3710
                     /locus_tag="PLD05_13275"
     CDS             2523..3710
                     /locus_tag="PLD05_13275"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOP78460.1"
                     /translation="MHILVANLGSTSFKYRLFDMTTERQLARGGVERIGAPESRCFVE
                     IQGQRHEESRHVPDHAEAVRQCLAQLTDPQRGCLKDAREVAAIGFKAVHGGRISGVQR
                     VTEDVLAAMEEMAEVAPAHNPPYIRAMRLLMEKLPEIPLVAAFETGFHSTIPERNRYY
                     AVPFEWADWGVRCWGFHGASHRYIAVRTAQLLGREDLRIISCHLGGSSSVCAIRNGQS
                     VFTSMGMSPQSGLPNNNRVGDFDPFALPVIMKRTGMSLSQVLSVLANESGLLGLSGVS
                     TDLRDILQAANQGNWRAQLAVDVFVASIRKYLGGSLVELGGADVLVFTGGIGENQPVI
                     RTAVCRGLTELGIVLDETKNQQAQGEAEIGHETSRTRIWVIPTNEELIVARQAKALLE
                     SGQ"
     gene            3713..4048
                     /locus_tag="PLD05_13280"
     CDS             3713..4048
                     /locus_tag="PLD05_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOP78461.1"
                     /translation="MFLAKVTGSLVSTQKVDSMVGRKLLLVEPYRVDPQTRSQLVGTG
                     RTFVAVDTVGAGEGELVLIVQGSSARLTPETKNLPVDTVIIGIVDHVHVEQRSVYSKQ
                     PEDRPKNSV"
     gene            4205..5650
                     /locus_tag="PLD05_13285"
     CDS             4205..5650
                     /locus_tag="PLD05_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOP78462.1"
                     /translation="MQINEALVKTVVEEVLHRLKGNGTPAAPPPVVKRFQGRLGLFTD
                     VDDAVAAAVEAYDMLSRRTLEERKRIIDHIRRIAIEQCVELGTMEMEETKIGRLEHKI
                     EKLRILGERTPGVEFLRSEVFSGDHGLTVIEHAPFGVIGAVTPVTHSLPTLTGNAINM
                     IAAGNTVVFNPHPSGKRVAAEGVRRYNSAIYRDLGIDNLICVITEPTIETANAIFKHR
                     DVALLCVTGGPGVVRVAMQQAKRAIVAGPGNPPVVVDETADLDRAARCIIQGAAYDNN
                     LLCIAEKEVLAVDKIFDQLMAAMERAGAVRLNASEIEALTAKAITYAGEGEHRHPVGV
                     KEFIGQDAAVLARAIGKKIPEDVELLFGETDFSNPFVHAEQMMPFVPFVRCRDVDEAI
                     EKARIAEHGFRHTAIIHSHDVVNMTKMGRALETTIFVKNGPSMAGLGLGGEGYLSYSI
                     AGPTGEGITTPLTFTRERRCAMIDSLRILGK"
     gene            5726..5983
                     /locus_tag="PLD05_13290"
     CDS             5726..5983
                     /locus_tag="PLD05_13290"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOP78463.1"
                     /translation="MLAGRVVGHATSTVKHPSMQGWRLLLVQPVAADGRSPDGDPILV
                     VDHLGAGRGDLVIVTSDGKHTQTVLKDAHTPVRWSVIGIVD"
     gene            5983..6816
                     /locus_tag="PLD05_13295"
     CDS             5983..6816
                     /locus_tag="PLD05_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78464.1"
                     /translation="MDSSHVDIQVIERIVREVLADLGRVSPIASEAPGQQSASRTHAG
                     EGKQSTDDVTSANGGTTLPRLARIHSPRDGELTLPNKVITLATLENRLTGVRQIVVPP
                     GAIITPAVRDLLAEKAIKVFFGIGPKSTEVPAKANNETGTPEGASLVILLVTRRVSTE
                     QLENLLRPEGITAAYESLDCLVRASDRVAEVCRSGNLAVVLTRHVSMEVCLANRHKGV
                     RAVAGRWGMDIAAETASVGANVLVADVTAGLFALKRMITAFVRASHVCPDNLRERLEL
                     S"
     gene            6877..7188
                     /locus_tag="PLD05_13300"
     CDS             6877..7188
                     /locus_tag="PLD05_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOP78465.1"
                     /translation="MRLGKVIGTVTLSRAHPTVRGARFKIVVPLSLKMLLEAPTSDAE
                     ELVIYDELGAHDGCLVAFSEGREAAQPFPEPKPVDAYLAAILDHVAAVPLEEVLPQPV
                     D"
     gene            7348..>8144
                     /locus_tag="PLD05_13305"
     CDS             7348..>8144
                     /locus_tag="PLD05_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOP78466.1"
                     /translation="MLNIHRVKQEICEIGDRLYKRGFAAANDGNISYRVSENQVVCTP
                     TMICKGFMKPDDLCVVDMEGRQISGVRKATSEIKLHLAIMKARPEVRAVVHCHPPHAT
                     AFGIAREAIPLCVLPEVEIFLGEVPIARYATPGTQEFADTILPFVEKCNVIILANHGT
                     VSFGDTVEKAYWYTEILDAYCRMLMLAKSLGRINYFTEPEAKALLELKQRLGYKDPRL
                     ELENCELCANDVFRDSWKESGVRQEAFQPPRFHSGAACPGGSVSEQER"
BASE COUNT         1759 a         2039 c         2608 g         1738 t
ORIGIN      
        1 gatttttgcc aattatgccc ggtttgcccc aatcctcgag cgttcaagag cggcgacagc
       61 aactgctcga gctaattcgc acgcggcggt ttgccacgct cgaggagttg gcggcagccc
      121 tcggtgtctc cgaatcgaca gtgcggcgtg acttggaggc cctagaacag cagggtgatg
      181 cgcgaaggat tcatggcggt gccctctata cgggaacttc gcccaaactg gcgcattttg
      241 acgtccgcca gcccatgcgg tgggaacaga aacgggctat tgcccgaacg gccgttgagt
      301 tagtctctgc gggggataca gttatcctcg atggtggcac gacgacatac gaggtcgcca
      361 ggctattgtt gggggtgccc atccacgtgg tgacgaattc cttgccggtg gcaaatttat
      421 ttgcctcaga cccagcgagt gacctagtgt ttgttggtgg gagtatttgt ccccgcacag
      481 gtgttgccca aggccctcac gctgaggcga tgattgcagg gcttcgggta agaaaggcca
      541 ttttgagcgt ggccgccgcc aacgacgagg gctttttcaa caacaacgtc ctcctcgtgg
      601 aaacggaacg ggccatgatg caggccgccg acgaggtcat cattgtggct gacagctcca
      661 aatttggtca ccgaagtctc atgcaccttt gcccactaga cgaggtggac tgcatcgtga
      721 cggatgaggg attgtccgac gagtggcggg cgaagatcac gagcttgggt gtgcgcctga
      781 ttgtggcccc actggctgaa gaggcagcgc ggggtgggac gacaaatccg ccagattgat
      841 cgtgtctgtg atggagggat tggcgacaga aaagcggcct gagttggccg ggaaatttta
      901 aacaacacga gacggaaggg cattcgatcc atgagtgttg cggcaccgat agatcgcggt
      961 caagtggaaa agatcgtccg tgaggtcgtc atcaagttat tgggagggcc cgcggaacgt
     1021 ccggaagtcg tggtcagtgt ctcagcgcgt cattgtcacc tatctcggga gcatatcgac
     1081 atcctttttg ggcctggcta tgagcttaag ccttacaaat ggttgtacca agaagggttc
     1141 tacgccgccg aggaaacggt catgattgtg ggtccgcggc ggcggatgtt gccaacgctt
     1201 cgtgtgctgg ggccgccacg gtcaaagacg caggtcgagt tggcctttac ggatgccatt
     1261 tctcttggaa tcgatgcccc agttcgtctg agtggtgatc tgaaggatac acccggatgt
     1321 gtgttgattg gtccggaagg aagcgtggac ttgaaggagg gcgtgattcg cgcggccagg
     1381 catgtccaca tgaacgtgcg ggatgcggcc ttctacggcg tgaagaatgg cgacgtcatg
     1441 tcgctacgtg tggagtcggg accttgcacg acggtgttcg aaggcttggt tgtgcgggcc
     1501 gacgagcaga gcaagcttga ggtccatctc gacacggatg aaggaaatgc ggccgacatc
     1561 gatcacgcca cccgcgtgga gttgttcaaa ccgtaaccgg aggaaataaa gtcaacaaag
     1621 tcaagaggtt cgtcagagtc gtcaatctga tgatgtaaat gatcggcacc ttttcgtgtg
     1681 gagtgaacgc ccatgaccaa acagatggaa gccctgggaa tgttggaaac gaaaggtttt
     1741 gtgacgctcg tggaagcgac cgatgccatg ctcaaggctg ccaatgtgca gttcttgggt
     1801 tgggacaaag tcggcagtgg attagtcagc gtcttcgtaa ccggtgatgt tgcggccgtc
     1861 aaagcggcga ccgatgcggg agccgctgcc gccagccgga ttggtgaggt cgtaagtgtg
     1921 caagtcattc ctcgacctca cgacgacctg aatgtggtgc tcccaacagc accggcaaaa
     1981 ggtggaaaaa ccgactgaga ccgtgttatg cctcggaaac agactcaaaa aagaaacttg
     2041 gccaacgatg aaataggggg atttcgcact atgcaagacg ccatcggatt gatcgaaacg
     2101 cgtggattag ttgctttggt tcaggctacc gatgccatgg caaaagccgc caatgtgcaa
     2161 atcgtgaagc gagtgcagat tggcggcggt ctggtgacga cgatcgttcg tggggacgtg
     2221 gggagcgtcc gtgccgcggt tgaagccggt gctaacgctg ccgcccaggt cggtgaattg
     2281 gtggccagtc acatcattcc gcggccagcc gagggtgtca tcgcggccta cttgacgtga
     2341 ttccctaggc catcttgtgt gtcggggccg acaccttccg ccgaaccaga ttcggtgtga
     2401 acaagtgttc ggtgatccgg caagaagatg ccgcctcggg tttcgcggtc agcatgacat
     2461 cagcggatgg cagtgatccg cagtatggga tgacgggaca agatttggaa ggaggtcgca
     2521 ggatgcatat cttagtggcc aatctcggaa gcactagttt caagtaccga ctgttcgaca
     2581 tgaccacgga gcgccagttg gctcgtggtg gtgttgagcg gatcggtgct ccggaaagcc
     2641 gttgctttgt ggaaatccaa gggcagcggc acgaagagtc ccggcatgtt ccggaccacg
     2701 ccgaggcggt tcgccagtgc cttgcccagt tgactgatcc gcagcgaggg tgcctcaagg
     2761 atgcccggga ggtagctgcc atcggtttca aggcggtgca cggaggccgc atctcgggcg
     2821 tccagcgagt cacggaggat gtgctcgcgg caatggagga gatggccgag gtagccccgg
     2881 cccacaatcc gccgtatatc cgggcaatgc ggctcctgat ggagaagctc cccgagattc
     2941 ctttggtggc ggcctttgag accggttttc acagcacaat ccccgagcga aaccgctatt
     3001 acgctgtgcc cttcgaatgg gccgattggg gtgtgcgttg ttggggtttt catggggcta
     3061 gccaccgcta cattgccgtt cggacagcgc agttgttagg ccgtgaggac ctacggatca
     3121 tttcctgcca tctcggtggg tccagttcag tgtgcgccat tcgcaatggg caaagcgttt
     3181 tcaccagcat gggaatgagc ccccaatcgg ggttgcccaa taataatcgc gtaggcgact
     3241 ttgacccgtt cgccttgccc gtgattatga agcggacggg tatgtcgcta tcacaggtgc
     3301 tttctgtgct ggcgaatgag agcggtttgc tcggtttgag tggcgtcagt accgatctcc
     3361 gcgatatcct tcaggcggca aaccagggga actggcgtgc gcaactggcg gtcgatgtgt
     3421 tcgtggcgtc gatccgcaag tacctcggcg gttcactggt cgaattgggc ggtgcagacg
     3481 tcctggtctt caccggcggg attggcgaga accaaccggt tatccggaca gccgtttgcc
     3541 gcggtttaac cgagcttggc atcgtgcttg atgagacgaa gaatcagcag gcacagggag
     3601 aggccgagat cggtcacgag acgagtcgca cccggatttg ggtgattccc accaatgagg
     3661 aattgattgt ggctcgacaa gccaaggccc ttttggagtc agggcaataa atatgtttct
     3721 cgcaaaagtg acgggttcgt tggtttccac ccagaaggtc gattccatgg tggggcgtaa
     3781 gctcctcctg gtcgagccct atcgtgttga tccacagacg cgatctcagt tggtgggaac
     3841 gggccgcacg tttgtggctg tcgataccgt cggggccggc gagggagagc ttgtgctgat
     3901 cgttcaggga tcgagcgccc gcttaactcc cgaaaccaaa aaccttcccg tggacaccgt
     3961 gatcattggc attgtggatc acgtccacgt ggagcaacgg agcgtctata gcaaacagcc
     4021 ggaggaccgg ccaaagaact cggtataagc ttggcgaagc agttgtggat ggccggggcc
     4081 gtgtgatgaa cggatgcatg tgcgtggtga tgacgtatga ttgcgagggt gaattcttaa
     4141 gttttgatga cgtcgggaag tgtgcgagga ttcccacgcg acgacatcca taaggagcgt
     4201 gaccatgcaa atcaatgagg ctcttgtgaa aacggtggtg gaggaagttt tgcatcggct
     4261 aaagggtaac ggaaccccgg ccgcaccgcc acctgttgtc aagcgatttc aaggccgtct
     4321 ggggctattt accgatgttg acgacgcggt ggctgccgcg gtagaggcct atgacatgct
     4381 ttcgcggcgg acgctggaag aacgaaaacg catcatcgac cacatccggc gaattgccat
     4441 cgaacagtgc gtcgagttgg gcacgatgga gatggaggag accaagatcg gccgactcga
     4501 gcacaagatc gagaagttac gcatcctcgg ggaaaggacg ccgggcgtcg agttcttgcg
     4561 gagtgaggta ttcagcggcg atcatggctt gacggtcatc gaacatgcgc ccttcggcgt
     4621 tattggtgcg gtcacgccgg tgacgcattc gttgcccacc ttaacgggca atgccatcaa
     4681 catgattgcg gcgggaaaca ccgtggtgtt caatccccat cccagcggca agcgggtggc
     4741 tgcggagggt gttcgtcgat acaactccgc catttaccgt gatttgggaa tagataactt
     4801 gatctgcgtc atcacggagc ccacgatcga gacggccaac gccattttca aacatcgtga
     4861 tgtggccctc ttgtgcgtga caggcggtcc tggggttgtc cgtgtggcga tgcagcaggc
     4921 aaaacgggcc atcgtggccg gaccgggaaa tccccccgtg gtggttgacg agactgccga
     4981 tctcgaccgg gcggcgcgtt gcatcattca aggcgccgct tacgacaata acctcctctg
     5041 catcgccgaa aaggaagtcc tcgccgtgga caagattttc gatcaactta tggcggcgat
     5101 ggaacgggct ggggctgtcc gtttgaacgc ctcagagatc gaggctctga cggccaaggc
     5161 catcacgtac gccggggagg gtgaacatcg ccatcccgtt ggggtgaagg agtttattgg
     5221 gcaggatgca gccgtcttgg cccgggcgat tggcaaaaag atccctgaag atgtggaact
     5281 cctcttcggg gaaaccgatt tttcgaatcc gtttgtccat gccgagcaaa tgatgccctt
     5341 cgtgccattt gtccgctgtc gggatgtcga tgaagcgatc gaaaaggcgc ggattgctga
     5401 acatggcttc cgtcacacgg caattattca ctcccatgac gtggtgaata tgacgaagat
     5461 gggacgggca ctagagacaa ccatctttgt gaaaaatggc ccgtcgatgg caggactggg
     5521 gcttggtggt gaaggctacc tttcgtattc cattgctggg ccaacaggtg aggggattac
     5581 gacgccgttg acattcaccc gggaacgtcg ctgtgccatg atcgacagtc tccgcatcct
     5641 cggcaagtga tgttgtttca ttcttaaacg agagaccgat ccgagatcga cgatggcaca
     5701 caacgggtga cgaaggcaat cgaacatgct tgcaggacgg gtcgtcgggc atgcaacgtc
     5761 aacggtgaag catccatcca tgcagggctg gaggctcctg ttggtacagc ccgtggccgc
     5821 cgacggtcgg tcgccagatg gcgatcccat tttggtggtg gaccatttgg gggcaggacg
     5881 aggggacctc gtcattgtaa caagcgatgg aaaacatacg cagacggtgt tgaaagacgc
     5941 ccacacacca gtgcggtggt cggtgattgg gattgttgac tgatggattc ttcccacgtt
     6001 gacatccaag taatcgagcg cattgtccgg gaggtcctgg cggatctcgg gagggtctca
     6061 cccattgcgt cagaggctcc cggtcagcaa agcgcttcga ggactcacgc cggcgaggga
     6121 aagcagagca ctgacgacgt aacgagcgcc aatggcggga cgactctgcc gcgtcttgcc
     6181 cggattcact cgccacgaga cggcgaactc acactgccaa acaaggtgat caccctggcg
     6241 acactggaaa atcgcttgac gggcgtccgg caaattgtcg ttccacctgg ggctattatc
     6301 accccggcgg tccgggatct tttggcagag aaggcaatca aagtgttttt tggcatcgga
     6361 ccgaaaagca cagaggtgcc agccaaggcc aataatgaaa cgggcacgcc ggagggtgcg
     6421 tcccttgtga ttttgttggt cacacggcga gtctccaccg agcaactgga aaacctactt
     6481 cgacctgaag ggatcactgc cgcgtatgag tcgttggatt gcctcgtgcg ggcaagcgac
     6541 cgtgtggccg aggtgtgtcg gtcaggaaac ttggccgtgg tattaacgcg gcatgtctcc
     6601 atggaagttt gcttggctaa caggcacaag ggagttcgcg ccgttgcagg tcgctggggt
     6661 atggacatcg ccgcggaaac ggcatcggtg ggcgccaatg tccttgtggc cgatgtcacg
     6721 gcagggttat ttgctctcaa acgaatgatt acggcatttg tccgtgcttc gcacgtttgc
     6781 ccggataacc tgcgggaaag attggagttg tcttaaataa aggcttggtt ttgtagcccg
     6841 gcagaagaat acagttagcg agggagagta acccctgtgc gtctgggaaa ggtgatagga
     6901 acggtgactc tgagccgcgc ccaccccact gttcgcgggg cgaggttcaa gattgtagtt
     6961 cctttatcgt tgaaaatgct tttggaggcc cctacctcgg atgccgagga attggtcatt
     7021 tacgatgagc tgggggccca cgatggttgt ttggtagcgt tcagtgaagg gcgtgaggcg
     7081 gctcagccct tccccgaacc gaaacccgtg gacgcttacc tggcggcgat cctcgatcat
     7141 gtggcagctg tgccactaga agaagtgtta ccgcagcccg tggattaaaa ctggaaggcg
     7201 ggcggaacgt cgtttttgag atgattggca aaggaattaa atattgaata cgagtgatgc
     7261 gttttggagg gttttgcgat ggcattgcaa gcggaaacca atagaaatca aggcggggcc
     7321 aaacggacga atggtatgcc gggcggcgtg ctgaacatcc atcgcgtaaa gcaggaaatc
     7381 tgcgagattg gagatcgttt gtacaagcga ggcttcgcgg cggccaatga cgggaatatc
     7441 tcctaccgcg tgagcgaaaa tcaggtcgtt tgcaccccaa ccatgatctg caagggtttc
     7501 atgaagccgg atgacctgtg cgttgtggac atggagggaa ggcagatttc cggggtacga
     7561 aaagctacca gcgaaattaa attgcatctg gccattatga aggctcggcc agaggttcgg
     7621 gctgtggtcc attgtcatcc accgcacgcg acggcgtttg gcatcgcccg ggaggcaatt
     7681 cccctgtgcg tcttaccgga agtggaaatc ttcctcggcg aagtgccgat tgcccgttat
     7741 gcgacgccgg ggactcagga attcgcggac accattttgc cgttcgtcga gaagtgcaac
     7801 gtcattattt tggccaacca tgggacagtg agcttcgggg atacggtgga aaaggcctat
     7861 tggtacacag agatcctcga cgcgtattgc cgcatgctga tgctggccaa aagcctgggc
     7921 cgaatcaatt atttcaccga gccagaggcc aaggccctgc tggagttgaa gcaacgcttg
     7981 ggctacaagg acccacgctt ggagctggag aattgtgaat tgtgcgcgaa tgacgttttc
     8041 cgggacagtt ggaaggaaag cggtgtgcgc caggaggcct tccaaccacc gcgtttccat
     8101 tcaggtgccg cttgcccagg tggctcggtc agcgagcaag agcg
//
LOCUS       DAOSUT010000174         8113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4547_length_8113_cov_9.294738, whole genome shotgun sequence.
ACCESSION   DAOSUT010000174 DAOSUT010000000
VERSION     DAOSUT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_8113_cov_9.294738"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLD05_13310"
     CDS             <1..969
                     /locus_tag="PLD05_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOP78467.1"
                     /translation="DRSKGAEAIRRHILELARPLIAQYHPEAIGIAFGGPIDAAKGRT
                     LRSHHVAGWEEFPLVEWCGSELGLPAVMGNDADLAGWAEATFGAGKGHRTVFYITVGT
                     GIGGALIHEGKIYTGAHGIAAEIGHLRPGLDAYSPKDDLESISAGWGIAAQAEAFINW
                     FLEQRAAKSPGEHIVQMALARWGLPQEASVPFEELDRAAEDILSRCNRNLGNLTTKLL
                     GEALADENLVAKAVFRKAIRTLGWAVAQMITLLAPSAVVIGGGVALLGEKLFFEPLRQ
                     EVERYVFPVLIGRYSISSATLGEEVMIHGATLLARNAFASAPDFAA"
     assembly_gap    1262..1361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1362..2204
                     /locus_tag="PLD05_13315"
     CDS             <1362..2204
                     /locus_tag="PLD05_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78468.1"
                     /translation="IGQQHHKFRMFYLTSDLSQRQSVITAFAYALFMYLPGYTVSQVT
                     VQRYLCVRNIRQARHALLINAVIITLTSLLFFLVGSTIFAYYNQPGKTGLPVLPKQDQ
                     IFPFFVMNELPAWGLTGVLLAGLFAAVMSTLDSGINSLAAVVAYDWLGGQKLSVTGSR
                     LLTLIFGATIVAAAVVVPAIAEHVIDIIAGIAGTFLGLLLGLFVLGMFFPRANTLGAA
                     IGLFAGIICLAWSISAKGIPHWWYGAFASLPIVVVGYFASCFAPPPNADQARGVAIGE
                     KKGS"
     gene            2240..3709
                     /locus_tag="PLD05_13320"
     CDS             2240..3709
                     /locus_tag="PLD05_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOP78469.1"
                     /translation="MVHVPRRKPLFARVGVFGVGHGTYWGQFPGLLDVMHQKLEKLCQ
                     KLVAYGVEVINFGLVDNAQGAYALLPRLQAADLDLVFCDMVTYATSSTFGVIIRNLSI
                     PLVLVALQPLAALDYPRATTFMQLCNDDFCAVPEFAGVAVRMGKRVPPLILGMLENDP
                     VADAELATWCQIAKVLRDLRTARIGLMGHVLEAMLDMHSDPTALTAAFGCHIVQVEPD
                     DLMRHHRDIPESLITEKIQEILKLFDTPEPQSDPITHKASEEDLRAAAKAAVALDRLI
                     EEKSLDGLAYYYEGEPGSPMRQLMANLIVGNSLLTAAGFPMCGEADLKTCIAMLILDR
                     LEAGGSFAEFHPIDFREGFVLVGHDGPHHINIAEGKPVLRSLDRYHGKPGSGVSVEFK
                     IREGPITLLGITQTNDGRCKFVVAEGQSIKGPIPATGNTNTRGYFLPDVRTFLRRWVA
                     EGPTHHFALAVGHLAETLREIADVLNLPCAIVARGDDGS"
     gene            complement(3935..5488)
                     /locus_tag="PLD05_13325"
     CDS             complement(3935..5488)
                     /locus_tag="PLD05_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOP78470.1"
                     /translation="MKTVDRNTPAVGIDLGTTYSVVAYLDAQGHPETLVNAEGERLTP
                     SVVFFDDEEVIVGREALKAFARLPERAAECSKRDVGYKVYHKVLQGKYYPPEVIEAYI
                     LNKLRQDAAQQIGDFSQVVITVPAYFDEVRRKATQDAGYMAGLDVMDIINEPVAAALA
                     FAYEAGFLSEASIPSRPERILVYDLGGGTFDVTVMEIRGHEYVTLAIDGDVRLGGWDW
                     DQRLVDLVAEAIIREHGFDPREDVRAWGKLWSLCEEAKRTLSVRPRATISFEYQGRTA
                     EIDVQREQFEAATQDLLDRTRFTTTQALKAAGLTWAEIDRVLLVGGATRMPMVRRLIR
                     DLVKKEPEVSVSPDEAVALGAAIHAGWLLGKAQGNPPRFRIRNVNSHSLGVVGIDPLT
                     RRPRVGTIIPRNTRLPIIAKRRFKTHRPNQRSILVRIVEGESPNPEDCCSIGQVVIRD
                     LPLNLPKGWPVEVIFHYRANGRLRVKIRVRHMEQDIVAEFRRERALTKELLDGWRRFI
                     SGKEPTDYE"
     gene            complement(5776..7740)
                     /locus_tag="PLD05_13330"
     CDS             complement(5776..7740)
                     /locus_tag="PLD05_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOP78471.1"
                     /translation="MGSKQRDLFLPTTPEEVAARGWDEVDVVFVTGDAYVDHPSFAMA
                     LLGRLMEAEGYRVAILSQPDWRSCEPWRQFGRPRLCFAVSAGNMDSMVNHYTANRKVR
                     NDDAYSPGGQIGLRPDRATLAYCQRAREAFKGVPVIAGGVEASLRRIAHYDYWSDKVR
                     RSILLDSKADLLVYGMGERPLLEILARLADGEDIKQLRDIRGTVYRLGASEPPVEDAL
                     MLPSYEEVVADKEAFARMTKMAHLESNPYNARRLVQYHGREAVVVNPPAFPLTEEEMD
                     RIYSLPFVRRPHPKYGDQPIPAFEVVKHSIQIMRGCFGGCTFCSITAHEGRIIQSRSP
                     DSILAEVNRIKEDRDFKGVISDIGGPTANMYRMNCSRPEVRAKCRRLSCLHPTICKLL
                     DTNHGPLIDLMKRVRMVKGVKIVFVASGIRTDLAQLSPAYLNQLIRHHIGGHLKVAPE
                     HCDRDVLRLMKKPSIEDFENFARKFYEISRAAGKEQYLVPYFISGHPGCDLKAAIRLA
                     EFLKRTGYRPQQVQDFMPTPFDIATCMYYTGIDPMTGEKVYVPKGERERRRQRALLQF
                     WKPENYFDVRDALKAAGRTDLIGEGPQCLIPSRPPREALLHERKKTGHHRGQLADESG
                     QSVDMGSRQKRLPAVGYRPHRATFRRRPLN"
     gene            complement(7837..8058)
                     /locus_tag="PLD05_13335"
     CDS             complement(7837..8058)
                     /locus_tag="PLD05_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78472.1"
                     /translation="MADLNGNQTPTRFLFDPDFCEKRCPICTRARRGVRWARWLQRLE
                     LFLTFGGCPWGRARAKKYGVPPDQPQPAS"
BASE COUNT         1718 a         2312 c         2141 g         1842 t
ORIGIN      
        1 gatcggtcaa aaggtgccga ggccattcgt cggcacatcc ttgagcttgc caggcctttg
       61 atcgctcaat atcatcctga ggcgattggg attgcttttg gcggccccat cgatgccgcg
      121 aagggacgca ccttgcgcag tcaccacgtg gccgggtggg aagagtttcc tttggtcgaa
      181 tggtgtggtt cggaattggg cctcccggct gttatgggaa acgatgccga tttggcaggt
      241 tgggccgaag ccacattcgg ggcaggaaaa ggtcatcgta ccgtctttta cattacggtc
      301 gggaccggca tcggcggtgc cttgattcac gagggcaaga tttataccgg tgcccacgga
      361 atcgcggcgg aaatcggtca tctccgcccg ggattggacg catattctcc gaaagacgac
      421 cttgaatcga tttcagcggg ttggggtatt gccgcgcagg ccgaggcttt tatcaattgg
      481 tttctcgaac aacgcgccgc caagtcccct ggtgagcaca tcgttcagat ggcactggct
      541 cgctggggac ttccccaaga ggcctcggtt ccctttgaag agttggatcg agcggcggaa
      601 gacatccttt ctcgctgcaa tcgcaactta gggaacttga caaccaaatt gcttggtgag
      661 gccttggctg atgagaactt ggtggctaaa gccgtattcc gcaaggcgat ccgcaccctt
      721 ggatgggccg ttgcccagat gatcacgctg cttgcaccct cggcggttgt cataggtggt
      781 ggtgtcgcgc tcctgggtga aaaattattt ttcgaaccct tgcggcagga ggtggagcgt
      841 tacgtttttc ccgtgctcat tggtcgatac tcaatttctt ccgcgactct tggcgaggaa
      901 gtgatgatcc acggggccac cttactggcc aggaatgcct tcgcatcggc tcctgatttt
      961 gcagcgtaat acgtcgcgcg gcacgttaat cgccgtaggt gagacaagtc acaaagggcg
     1021 aggcgcggtt cgttcctttt ggcgacacga gttggcgctg ccgtacttaa ggtctatccg
     1081 aataagtccc cctctccctc cgggagaggg ctggagtgag ggtatgcgaa cgccggaatc
     1141 gattgagaaa accggcagcg attcgagcca ccctcatcct ccatccctct cccgccgaac
     1201 gcgtgagggg agattatggg ttagggtctt aactatttca ttgaccgatc tccacaacgc
     1261 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcggccaa cagcatcaca
     1381 aattccgcat gttttatctg acatctgatc ttagtcagcg tcaaagcgtc attacggcgt
     1441 ttgcgtatgc cctgttcatg tacttgcccg gctacactgt ttcccaggtc acggtgcaac
     1501 gttatttgtg cgtcagaaac atccgtcaag ctcgacacgc actcttgatc aacgccgtga
     1561 tcatcacatt gacatcgctg ttgtttttcc tcgtgggaag taccattttt gcttactaca
     1621 accagcctgg gaaaaccggc ctacctgtcc tgcccaaaca ggatcagatt ttccccttct
     1681 tcgttatgaa cgagctaccg gcctgggggt tgacgggcgt actcctggcg ggactatttg
     1741 ccgccgtgat gagcacgctc gacagcggca ttaacagttt ggcggcagtt gtggcttacg
     1801 attggctcgg ggggcagaaa ctctcggtca cagggagccg cttgctaaca ttgatttttg
     1861 gggcgaccat cgtggcagcg gccgttgtcg tgccggccat tgccgaacac gtcattgata
     1921 ttatcgccgg aattgcaggg acatttcttg gtttgctcct gggcttgttt gtcctcggca
     1981 tgttctttcc gcgcgcgaat accctcggtg cggccatcgg attgtttgcc gggattatct
     2041 gtttggcctg gtcgatctcg gcaaagggca ttccgcattg gtggtatgga gccttcgcga
     2101 gtctccccat cgttgtggtg ggttatttcg ccagttgctt tgcgccccca ccaaacgccg
     2161 atcaagcccg tggcgtcgcc atcggagaga aaaaagggag ttaggagccg tgcgtgaaaa
     2221 tagttcgtcg aggccgtcca tggtgcatgt tccccgtcgg aaacctcttt ttgcccgggt
     2281 gggcgtcttc ggtgtgggac acggcactta ttggggacaa tttccgggtt tgctcgatgt
     2341 catgcaccag aagctggaaa agctctgtca gaagcttgtg gcctacggcg ttgaggtgat
     2401 caactttggt ctggtcgata atgcccaggg ggcgtacgcc cttcttccac gactccaagc
     2461 ggcggatctc gacctggttt tctgtgacat ggtgacctac gctaccagtt ccacgtttgg
     2521 cgtcatcatt cgcaacttgt cgattccctt ggttctggta gccctgcaac ccttggccgc
     2581 actggactat ccccgggcga ccacgtttat gcaactgtgc aatgacgact tttgtgcagt
     2641 tcccgaattt gcaggtgtgg ctgtgcggat gggcaaacgg gtaccgcctt tgatcctcgg
     2701 catgctcgag aacgaccccg tggcggatgc cgagttggca acgtggtgtc aaattgccaa
     2761 agtcctgcgc gatttgcgca cagcacgaat tggcctaatg ggtcatgtcc tcgaggctat
     2821 gttagatatg cacagtgacc ccacggctct taccgcggcc ttcgggtgtc acattgtgca
     2881 ggttgaacca gacgatttga tgcgacacca ccgcgatatc ccggaaagtc tgatcacgga
     2941 aaaaatccag gaaatcctca agctctttga cactccagag ccccaatcag accccatcac
     3001 ccataaagcg agcgaagagg atctgcgggc tgccgcaaag gccgccgttg cgcttgaccg
     3061 cttgattgag gaaaaatcac ttgatggcct ggcatattac tacgagggag aaccgggaag
     3121 tccgatgcgg cagttaatgg ccaacttgat tgttggcaac tccctattga cggcggccgg
     3181 ttttcccatg tgtggcgagg ccgacctgaa gacctgtatt gccatgctca ttttggaccg
     3241 tttggaggcc ggcggcagtt tcgccgaatt tcatcccatt gatttccgcg aaggctttgt
     3301 ccttgtggga cacgatggtc cgcatcatat taacattgcc gaaggcaaac ccgtattgag
     3361 aagcctcgat cgctatcacg ggaagccggg gtcaggggtc agcgtggagt tcaagattcg
     3421 tgagggcccc atcacacttc tcggaatcac ccaaacaaac gatggtcgct gcaaatttgt
     3481 cgttgctgag ggtcaatcca taaaaggtcc tattcctgcc acgggcaaca ccaacacacg
     3541 gggttacttc ctccctgatg tgcggacgtt tctgcgccgg tgggtggcgg aaggccccac
     3601 gcatcatttc gcgctcgccg tggggcattt ggcagagaca ctgcgcgaga ttgccgacgt
     3661 cctcaacttg ccctgtgcta tcgtagcccg cggtgacgac ggatcgtagc cgattgatta
     3721 agagccatcc caaaaaagcc tactctccct ccgggagagg gataaggtga gggtgagcga
     3781 acgttagaat cgattgagaa aaccggcagc gattcgagcc accctcaccc ccgacccctc
     3841 tcccgctgaa ggggagaggg gagattatgg catagggttt aagccaatcg gatgcctgaa
     3901 aaaaagtgac actccgtctc tgcggtaaca acgcttattc gtaatcggtt ggttccttac
     3961 cactgataaa tcgccgccag ccatccaaca actctttcgt cagggctcgt tctctccgga
     4021 actcggcgac gatatcctgc tccatgtgcc gtacccgaat tttcactctc aggcggccgt
     4081 tagcacgata atggaaaatc acctcgaccg gccaaccttt aggaaggttc aaaggcagat
     4141 ccctgatgac cacttgaccg atggaacagc agtcctccgg attggggctt tcgccttcga
     4201 caattcggac gagaatggaa cgctggttgg gccgatgtgt tttgaaacgc cgcttggcaa
     4261 tgattggcag ccgcgtattt cggggaatga tcgttcccac ccgcggccgc cgtgtgaggg
     4321 gatcaattcc caccacgcca agactgtgcg agttaacgtt gcgaatgcga aatcgcggcg
     4381 ggtttccttg ggctttcccc aatagccagc cggcatgaat cgcggcccct aaggcaaccg
     4441 cctcatcagg ggacacagac acttcgggtt ccttcttgac taggtcccga ataagccggc
     4501 ggaccatagg catgcgagtg gcaccaccaa caagaagcac gcggtcaatc tcggcccagg
     4561 ttaaccccgc cgccttcagg gcttgagtcg tcgtaaagcg ggtgcgatcg agaaggtcct
     4621 gtgtggccgc ttcaaattgt tcccgctgaa cgtcaatctc cgccgtccgc ccctggtatt
     4681 cgaaggaaat cgttgctcgc ggccgcaccg atagagtccg tttggcttcc tcacagagcg
     4741 accacagttt accccacgcg cgaacatcct cccgcggatc aaaaccgtgc tctcgaatga
     4801 ttgcctcagc caccaaatcg acaagacgtt gatcccagtc ccatccccct aaccggacat
     4861 caccgtcaat ggccaatgtc acatactcgt gtcctcgaat ctccatcacg gtcacatcaa
     4921 acgtccctcc ccccagatca tagacaagaa tccgctccgg ccgcgaggga atcgaagcct
     4981 cactgaggaa acccgcttca taagcgaatg ccaacgcagc cgccaccggt tcgttgatga
     5041 tatccatcac atccaacccg gccatgtaac cagcatcttg ggtcgctttg cgccgcactt
     5101 catcgaagta ggccggaacc gtgatcacca cttgtgaaaa gtccccaatc tgctgggcgg
     5161 catcctggcg gagtttattg aggatataag cctcaatcac ttcgggcgga tagtatttcc
     5221 cctgcagaac cttgtgatag actttgtaac cgacgtctcg tttggaacac tcggccgcac
     5281 gctcgggaag ccgggcaaac gcctttaagg cctcgcgacc aactatgacc tcttcatcat
     5341 caaaaaacac aacgctcgga gtaagcctct caccttcggc attgacaagg gtttctggat
     5401 gcccttgggc atctaaataa gccacaaccg aataagtcgt ccccagatca attccgacgg
     5461 ccggtgtatt tcgatcgaca gttttcattg gcgtttccag ctacgcaagt tgcccgatca
     5521 tcctcggctt cttgactccc ctatagctcc cagatattta acggccctac cgttcgcaac
     5581 gaatcattct ccgctcatag gagagccgtc taacatcaat gttaccttgt cagagttcgc
     5641 tcggggcaag agtgaacccc agagagaacc gcggggttac acaaccgggc agattcctga
     5701 gcattggctt tacaaagcag accgattatg caggatcgtc cggattttca ggcaatcgac
     5761 tgtccccaaa tcatgtcaat tgagcggtcg gcggcggaag gtagctcgat gcgggcgata
     5821 accgacggcc ggcaaacgct tttggcgtga ccccatgtca acgctttgcc ctgactcatc
     5881 cgccaactgt ccccggtggt ggccggtttt ctttctctca tgcagcaggg cctcccgggg
     5941 tggccgactg ggaatcaaac actgcggtcc ctcaccgatc aagtcggttc gcccagcggc
     6001 tttaagggcg tcgcgcacat caaaatagtt ttcaggtttc caaaattgta aaagggcccg
     6061 ttgccgtcgc cgctcccgtt cgccctttgg aacataaacc ttctcgcccg tcatcgggtc
     6121 aatccccgta tagtacatgc acgtggcgat atcgaagggc gtaggcatga aatcttgtac
     6181 ctgttgcggc cgataaccgg tccgtttgag aaactcagcc aaacggatcg ctgccttcag
     6241 atcgcaaccc ggatgtccgg aaatgaaata cggcacaagg tactgttctt ttcccgcggc
     6301 acgcgaaatc tcataaaact tgcgggcaaa gttttcaaaa tcctcgatgg aaggcttttt
     6361 catcagtcgc aagacgtcac gatcgcaatg ttccggagcc actttcaaat ggccaccaat
     6421 atggtggcga ataagctgat tcaggtaggc agggctgagc tgagccagat ccgtgcgaat
     6481 ccccgacgca acaaagacta tcttgacccc tttcaccatt cggactcgct tcatgagatc
     6541 gatcagtggt ccatggttcg tgtcgagcaa cttgcaaatg gtgggatgga gacaacttaa
     6601 tcgacgacat tttgcccgca cctccggccg actgcaattc attcgataca tattcgctgt
     6661 ggggccgcct atgtcgctga tcactccctt aaagtctcga tcctccttga tgcggttgac
     6721 ttcagcaaga atagaatccg gcgaacggct ttgaataatc cgcccttcat gtgccgtgat
     6781 cgagcaaaat gtgcatcccc caaaacaccc tcgcatgatc tgaatggaat gcttgaccac
     6841 ctcaaaggcc gggatcggct gatcaccgta cttcggatgc ggtcgtctca caaaaggtag
     6901 gctgtaaatg cggtccatct cctcttccgt gagaggaaac gccggaggat tcacaacgac
     6961 cgcctcccgc ccatgatatt gcaccaaacg ccgagcattg taagggttgc tttccagatg
     7021 cgccattttt gtcattcggg caaaagcctc tttatcggcg actacctcct cataggaggg
     7081 aagcatcaag gcgtcttcca ccggcggttc cgacgccccc agccgataga ccgtcccgcg
     7141 gatgtcacgc aactgcttga tatcctcccc atctgctaac cgggccagaa tttcgagaag
     7201 gggccgttcc cccatgccat aaaccaacag gtcggctttt gagtctaaca agatcgaccg
     7261 gcggaccttg tcgctccaat agtcataatg ggcaatgcgt cgcaaactcg cctcgacgcc
     7321 acctgcgatc acggggaccc ctttgaacgc ctctcgggcc cgttgacagt aggctagggt
     7381 cgcgcggtca ggacgcaatc cgatctgtcc gcccgggctg taggcatcgt cattacggac
     7441 cttcctattg gctgtgtagt gattcaccat cgagtccata tttcccgcac tcacggcaaa
     7501 gcacaggcga ggccgcccaa actgccgcca cggctcgcag gagcgccagt ccggctggct
     7561 cagaatcgcg actcgatagc cttccgcctc catcaacctc ccaagcagcg ccatagcaaa
     7621 gctcgggtga tcgacgtagg catcaccggt gacaaagacg acgtccactt cgtcccaacc
     7681 ccgggctgca acttcttcgg gcgttgttgg taggaatagg tcacgttgtt tacttcccat
     7741 gctccactca cttaaccact gaagcccctt gttgtttccg tcttgagaaa tacacgctca
     7801 ccggcgatcg accgatggct tccgtgaatt gtatccttag gaagcgggct gaggttggtc
     7861 aggtggcacg ccgtatttct tggcccgcgc tcgcccccag ggacaaccgc caaaggtcaa
     7921 gaaaagctcg agcctctgca gccatcgtgc ccagcggaca ccccgccgtg cgcgtgtgca
     7981 gattgggcac cgtttttcac aaaagtcggg atcaaacaga aaacgcgtcg gcgtttggtt
     8041 tccgtttaag tcagccatgg cttaacgcct tccttcaaat ggcgggacgt gcgatgataa
     8101 cttccaccat cgg
//
LOCUS       DAOSUT010000175         8078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4566_length_8078_cov_10.335161, whole genome shotgun sequence.
ACCESSION   DAOSUT010000175 DAOSUT010000000
VERSION     DAOSUT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_8078_cov_10.335161"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1720
                     /locus_tag="PLD05_13340"
     CDS             98..1720
                     /locus_tag="PLD05_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78473.1"
                     /translation="MCANTRVWFFGLTGGAGRWLWIIASWFCQRRLGLYVPVVLTLAL
                     ASFGLLWVPGAFAVETAPAMKAGFAERDISPDLGMEQPGGYGKSYHKVFHDPCKVRAS
                     VFDDGVKQVAVVGLDVLIIREPSVKRIREDVAQRTGLDPKAILLAASHTHSGGPTGMV
                     LPGEFDHADDWVKHLAYEVASAADARYLEKVEKLTVEAICEAWEKRHPVLCGVGRGIE
                     DQVAFNRRFRMKNGLTYTHPGQLNPEIVEVAGPTDPEVTVLGVWSPDGKLEGCVVNFA
                     CHATTSPGGISANWIYYMEKAIRGFFGEHVTVVFLAGCCGDVTQVNNLSPYQNPTGEE
                     WAKLVGGRVGAEAVKVLLSMPRGQLTPIDVRVSELVIPRRKPDPKRVEECLALTKKDP
                     KEADATKWIFAVQTVLLDAKIKVEPEARVELQAIQLGPLVILANPAEYFCQYGLDLKA
                     KSGFPFTMPVELANGCVGYVPTVDAFGPNGGGYETRLTAYSNLEFTAGDQIRDALLSL
                     AQQLSPGPVPERPKVTPVTRPWSYGSVPPNYH"
     gene            complement(1766..3214)
                     /gene="hpnE"
                     /locus_tag="PLD05_13345"
     CDS             complement(1766..3214)
                     /gene="hpnE"
                     /locus_tag="PLD05_13345"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOP78474.1"
                     /translation="MSTGEATGGNTNRDTRRVAIVGGGLAGLSAAWYLSKSGLPVTLF
                     EASSRLGGRARSFEERHIGCEVDTCQHIALGCCTQFFEFCRELQILPYFERHQTLYFV
                     DTTGQIHRFCPSRWLFPPLHLLPAVAKLRFLRPADRWRLLRGMSRLWRASTKDFWSTM
                     GEWLRANGQTAALVEHFWTPILISALSETLEYIPVVIARKVLVEGLLSTRDGYQLWTP
                     TRPLSHIFDGEVSTRLATAGVAIVKGAVVKGIRQKDTSWTLCFASGEEQTFDGIVLAV
                     PWRTAIRLLPPQLRESVFSGWVERLHPLSDAQDACAITGIHLWFDRPCLSLPHAILLG
                     RHSQWVFRPHFGQKVPVQEAPDGRSALGVTGDRHACYYQIVVSASHRMPRLTVDEWLK
                     VAVEDVKAVFPSAREARLLHGRVVTEPAAVLSPSEEWQRLRPPQMTPFVGLVLAGDWT
                     ATGWPGTMESAVRSGRAAAQAIVQSLRQGRPL"
     gene            complement(3239..4213)
                     /locus_tag="PLD05_13350"
     CDS             complement(3239..4213)
                     /locus_tag="PLD05_13350"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOP78475.1"
                     /translation="MPRIDPPDSQLAVSYAFCRAICRAAGSSFYPAFSMLSQEKAQAM
                     ETIYAFCRFTDDLVDDKLSVDPAQDLETWYQWVHGALVRGDRSWGELHSDLGTSASPV
                     SRGRALLPALVDTVEQFRIPPESLLRVIDGVRSDLTPRHVATFAELEDYCSKVASAVG
                     VACLCVWGMETQSIPPEAHDCGVAFQLTNILRDLAEDVKLGRIYLPQEDFVRFRCSLE
                     DFAARRVTPQWLALLDFEIERARNYYRRAAHIHGLLAPDGRRIFGLMWDVYWQLLKEI
                     ARRKEQLLERRVRLSRLRKSLLWLRWMVFPPKKLIEPTSPQESGSVKG"
     gene            complement(4229..5212)
                     /gene="hpnC"
                     /locus_tag="PLD05_13355"
     CDS             complement(4229..5212)
                     /gene="hpnC"
                     /locus_tag="PLD05_13355"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOP78476.1"
                     /translation="MMASKQSRLSTISPPGSSRDRDRFFADLAQFGPTAQYQPLSLRA
                     SRRYCRQLARSHYENFTVVSLFVPRDLRQHFCNIYAWCRWADDLADETGDPRWGMDLL
                     QWWQQELLACYQGVMRHPVLVALHETVTRFNIPRDPFLDLLTAFRQDQRVFRYETFEE
                     LLDYCRHSANPVGRLILFLGRCHTPDRVMLSDQICTGLQLVNFWQDVGQDYDRGRIYI
                     PKAECRRVGYTEEMFARREFNSAFRTLMKGLVHEAAAFLRRGRPLVAMMPRKLQIPVY
                     LFVRGGLAIARAIESHDYDVWTHRPTLSRSKKVELMIRTVWDVLCRRYDRD"
     gene            5311..6243
                     /gene="ispH"
                     /locus_tag="PLD05_13360"
                     /gene_synonym="lytB"
     CDS             5311..6243
                     /gene="ispH"
                     /locus_tag="PLD05_13360"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOP78477.1"
                     /translation="MRILLAAPRGFCAGVNMAIETLERVLRRYGPPLYVYHEIVHNRW
                     VVNEFVQRGVHFVESLDLVPNGGIVIFSAHGVSPEIRREAAARNLHVIDATCPLVTKV
                     HREVQRFAAMGYTILLIGHAGHDEIIGTMGEAPDQIRLIKDEEEALTVDVPDPEHVAY
                     TTQTTLSVMDAARIVECLRRRFPKLVGPARDDICYATQNRQEAVLELAREADVFLVVG
                     SQNSSNSRRLAEIAQRTGIPAYLVDGPEDLKKEWFSAEQTVGITAGASAPEHLVQGCI
                     EFLRAHFFATVEERVIRTENVHFQLPPELRQDQP"
     gene            complement(6249..>8078)
                     /locus_tag="PLD05_13365"
     CDS             complement(6249..>8078)
                     /locus_tag="PLD05_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78478.1"
                     /translation="PGFCREIHVALRGNDSNDGTPERPLRTISAAAQLAQPGDTVIVH
                     AGIYREEVAPPRGGLSDEQRITYQAAPNEQVEIRGSEVIKGWTKVEGDVWQVVIPNTF
                     FGNFNPYADVIKGDWFSPRGRVHHTGAVYLNGEWLAEAASKEDLFKPSSGLRWWFAEV
                     DEEKTTIWAQFPGVDPNRETVEINVRQTVFYPRTPGCNYITVRGFIMRHAATPWAPPT
                     AQQIGLIGTHWSKGWIIENNVISHSICCGITLGKYGDEFDNTSANTAEGYVQTINRAL
                     ANGWHRDNIGHHIVRNNIISHCEQAGIVGSLGAAFCEVTDNVIHDIHMRRLFSGAEMA
                     GIKFHAAIDTLIARNHIFRTCRGLWLDWMAQGTRVHANLFHDNDEDLFVEVNHGPFVV
                     DNNLFLSKLNLRDMSQGGAYLHNLFAGAITVCPEPNRKTPFHPPHKTEVAGLASIQGG
                     DNRFLNNVFIGRGENTPMPEGAPAPRRTWITGFGLSAYTAQAMMNLTEGNIYLDHAPP
                     AQNEANSLEIKEAPFCRLMADEGMVFLTLTTSADWANAGREIVTAARLGQTAVSQLPY
                     ENYDGRNLVIDTDYFGRPRTAPTLAGPFSQLEVGEVTLKVWPH"
BASE COUNT         1755 a         2251 c         2395 g         1677 t
ORIGIN      
        1 gcgtatcatt tacaacggct gacgacgtgg gtgaccgcgg caaagctcgc ggaatctcaa
       61 aggtgacggt ggttgtgttg ctaacaggag tgtagctatg tgtgcgaaca cacgagtgtg
      121 gtttttcggg ctgacgggcg gcgctgggcg ctggttatgg atcatcgcaa gttggttctg
      181 tcaaagacgt ttgggacttt atgtgccggt ggtgctaacg cttgctttag catccttcgg
      241 tcttttgtgg gttccggggg cctttgctgt cgagacggct cccgcaatga aggcgggttt
      301 tgccgaacgg gatattagtc ccgatttggg aatggagcag cctggcggtt acggcaagtc
      361 gtatcacaag gtgtttcatg atccgtgcaa ggtccgcgcg tcggtgttcg atgacggggt
      421 gaaacaggtg gccgttgtgg gcctggatgt cctgatcatc cgagagccga gtgtcaaaag
      481 gattcgcgag gacgtggcgc agcgcacggg tctcgatccc aaggccattc tcttggcggc
      541 gtcgcacacg cattcgggcg gcccaaccgg gatggtcctc cccggtgagt ttgaccatgc
      601 ggatgattgg gttaaacacc tggcttacga agtggcgtcg gcagccgatg ccaggtatct
      661 ggaaaaggtc gaaaaactga ccgtggaggc catttgtgag gcctgggaaa agcgacatcc
      721 cgtcctctgt ggcgtggggc gcgggatcga agaccaggtc gcgttcaatc ggcgattccg
      781 catgaagaat ggtttgacct acacgcatcc cggacaactg aacccggaga ttgtggaagt
      841 cgccggtccg accgatcccg aggttacagt cctcggtgtg tggtcgcccg acgggaaact
      901 ggaaggctgc gtcgtcaatt ttgcctgtca cgcaacgacc agcccgggcg ggatttccgc
      961 gaactggatt tactatatgg aaaaggccat tcgcggattt tttggagagc acgtgacggt
     1021 ggtctttctt gcgggttgct gtggagatgt gacgcaggtt aacaatttga gtccttatca
     1081 aaacccgacg ggcgaagaat gggcgaagct cgttggtggt cgtgtcgggg cggaggccgt
     1141 caaagtgctg ctttccatgc cgcgaggcca gttaacgccc attgatgtcc gcgtgtcgga
     1201 attggtcatt cctcgccgca agccggatcc aaagcgggta gaggagtgcc tggcattaac
     1261 gaaaaaggac cccaaagagg ctgacgccac gaagtggatc ttcgccgtgc aaacggtcct
     1321 tttggatgcc aagatcaagg tggagccgga agccagggtg gaactccagg ccattcaatt
     1381 gggaccgttg gtcatcctag ccaatcccgc cgagtacttc tgccagtacg gcctggattt
     1441 gaaggccaaa tccggctttc ccttcaccat gccggtcgaa ttggccaacg gatgcgtggg
     1501 ctacgtgccc acggtggacg cctttggacc taacgggggt ggttacgaaa cacgattgac
     1561 ggcgtatagc aatctggagt tcacggccgg cgatcagata cgagatgcgc tgttgtctct
     1621 ggcccagcag ttgagcccgg gccctgtccc ggagcgtccc aaggttaccc cagtgacgag
     1681 gccctggtca tacgggagcg ttccaccgaa ttatcattga gacttcgcac cgaaaagtgg
     1741 aattaagcat ccgcgaagtc ggcacctaaa gaggtctgcc ctggcggaga ctttggacga
     1801 tggcctgcgc ggcggctcgt ccgctgcgaa ccgcgctctc catcgtgccg ggccacccgg
     1861 tggctgtcca atcccctgcc aggacaagac cgacaaaggg ggtcatttgt ggcggacgta
     1921 ggcgttgcca ctcttcgctc ggggaaagca cggcggcggg ttccgtgacc acccgaccgt
     1981 ggagcagcct cgcctctcta gccgagggga agacagcctt cacgtcctcg acagccactt
     2041 tgagccattc gtccactgtc aggcgcggca tccggtgcga agcgctgacc acaatctggt
     2101 agtagcaggc gtggcggtcc ccagtgacgc caagagcaga acggccgtcg ggggcctctt
     2161 ggacggggac cttctggcca aagtgtggcc gaaaaaccca ttggctgtgg cgccccagga
     2221 gtatggcatg cgggagcgag agacacgggc gatcgaacca aaggtgaatt cccgtgatgg
     2281 cgcaggcgtc ctgggcatcg gatagtggat gaagtcgctc cacccatccc gaaaagacgc
     2341 tctcacgaag ttggggaggc aggagcctga tcgcagtccg ccatggcaca gcaagaacga
     2401 tcccatcaaa ggtttgctcc tcgccgctgg caaaacagag cgtccacgat gtgtcctttt
     2461 gacggatacc tttcaccacg gcgcccttga cgatggccac accggctgtg gccaggcggg
     2521 ttgaaacttc tccatcgaag atgtgactta agggtctggt cggtgtccaa agttggtagc
     2581 catcgcgggt ggagaggagg ccttctacca gcaccttgcg ggctatgacc accgggatgt
     2641 actccagggt ttcgctgagg gcactgatga ggatgggagt ccaaaagtgt tccacgaggg
     2701 ccgctgtctg gccattcgcg cgaagccatt cccccatggt tgaccaaaaa tcttttgtag
     2761 aggcccgcca aagtcgggac attccccgga ggagccgcca ccggtctgca ggacggagga
     2821 atcgcagctt ggctacggca ggtaaaaggt gcaagggggg aaagagccaa cggctgggac
     2881 aaaagcggtg gatttgccct gtggtatcga caaaataaag tgtctggtgt ctctcgaaat
     2941 aaggcagaat ttgtaactcg cggcagaatt cgaagaattg tgtgcagcac ccaagcgcga
     3001 tgtgctggca ggtatccact tcgcacccga tgtgccgctc ttcaaaagat cgggcgcgcc
     3061 caccaagccg gctgcttgct tcgaagaggg tcacgggaag gccggactta ctcaaatacc
     3121 aagcggctga aaggcccgcc aaacccccgc cgacgatcgc cacccgccgc gtgtcgcgat
     3181 ttgtatttcc tccggtagct tccccagttg acatggttgc atcctcaagc ggtgggagtt
     3241 agcctttcac agaaccactt tcctgtggcg acgttggctc aatgagtttt ttcggcggga
     3301 agaccatcca acgcaaccag agaagagact tccgcaaccg agataatcgc acacgccgtt
     3361 ccagtaattg ctcttttcgt cgggcgattt ccttgagcag ttgccaatag acatcccaca
     3421 tcaggccgaa aatccgacgg ccatcgggcg ccagtagtcc gtggatgtgc gccgcccggc
     3481 ggtaataatt gcgggcgcgt tcgatctcaa aatccaagag ggccaaccat tgcggcgtga
     3541 cccgccgtgc tgcgaagtct tccagcgaac aacgaaagcg gacgaaatcc tcttgcggga
     3601 gatagatgcg tcctagcttt acgtcctccg ccaggtcgcg gaggatgttc gtcaattgaa
     3661 aggccacgcc acagtcatgg gcttcgggcg ggatactttg tgtttccatc ccccagacgc
     3721 acaagcaggc cacacccaca gcggaggcca ccttgctaca gtagtcctct aattcggcaa
     3781 atgtggccac atgtcggggc gttagatcgc tgcgcacacc gtcgatgaca cgcagcagtg
     3841 actccggagg gatgcgaaac tgctccaccg tatcgacgag ggccggaagt agcgcacgtc
     3901 cccgggaaac cggtgaagcc gatgtgccga ggtctgaatg cagttcaccc cacgagcggt
     3961 ccccacgcac aagcgccccg tggacccatt ggtaccacgt ttccaggtcc tgcgccggat
     4021 caacggacaa cttgtcatcg accaggtcgt ccgtgaaacg gcagaaggca tagatggtct
     4081 ccatggcctg ggctttttcc tggctgagca tggaaaaggc gggataaaag ctggatcccg
     4141 ccgcccgaca gatggcacgg cagaaggcgt aactgacggc aagctgtgaa tcaggtggat
     4201 caatccgtgg catcgaatgg ctgcgccttc aatctcgatc ataacggcga caaaggacat
     4261 cccacacggt gcgaatcata agctccactt tcttgcttcg ggataacgtg ggtcggtggg
     4321 tccacacatc ataatcgtgg ctttcaatag cccgggcgat ggctagccca ccgcggacga
     4381 acaagtaaac ggggatctgg agtttcctcg gcatcatggc caccaaggga cggcctctcc
     4441 gcaagaaggc agccgcctca tggaccagcc ctttcataag cgtgcgaaag gctgagttaa
     4501 attctcgccg ggcaaacatc tcctcagtgt aaccgacgcg ccgacactct gccttcggga
     4561 tgtaaattcg cccgcgatca taatcctggc caacgtcttg ccaaaagttg accaactgca
     4621 aacccgtgca aatctggtcc gacagcatca cccgatccgg cgtatgacat cgtcccaaga
     4681 acaggatgag ccgtcccacc gggttcgccg aatggcggca gtaatccaga agttcttcaa
     4741 acgtttcgta gcggaatacc cgctggtctt ggcgaaaggc ggtgagcagg tcgagaaatg
     4801 ggtctcgcgg aatattgaac cgtgttaccg tctcgtgcag ggccaccagc acgggatgtc
     4861 gcatgactcc ctgataacag gccaggagct cttgttgcca ccactgaagg aggtccatgc
     4921 cccatcgggg atcacccgtt tcgtccgcca aatcgtccgc ccagcggcac catgcgtaga
     4981 tattgcaaaa gtgctgccgt aaatcccgcg gcacaaagag actgacgacg gtaaaattct
     5041 catagtggct tcgggcaagc tgacggcaat agcggcggct tgcccgcagg gaaagcggct
     5101 gatattgtgc cgtcgggccg aactgtgcca aatcagcaaa aaagcgatcc cggtcccgcg
     5161 atgaaccagg cggcgaaatg gtcgaaagtc gggactgctt tgaggccatc atgcgtcaaa
     5221 gtgaaacgga ataacggcaa aacaggcaaa aatccgcagt agtccctgcg ttagtgatca
     5281 ttatcctctg aaaggatcat tgagaaaacc atgcgaatcc tgctagctgc tccgcgcgga
     5341 ttttgcgccg gggtcaatat ggcgattgaa accttggaga gggtcctccg gcgttatggc
     5401 ccaccccttt atgtgtatca cgagattgtc cacaatcgct gggtggtgaa cgaatttgtc
     5461 cagcgagggg tgcactttgt ggaatccctc gacctggttc ccaacggtgg aattgtgatc
     5521 ttttcggccc acggtgtttc gcccgagatt cgccgcgaag cagcggcccg caatcttcac
     5581 gtcattgacg ccacgtgtcc cttggtgacg aaggtccacc gtgaggtgca gcgatttgcc
     5641 gccatgggat acacgatctt actcattggc cacgccggac acgatgaaat tatcggtact
     5701 atgggcgagg cccctgatca gattcgcctc attaaagacg aggaagaggc attaacggtt
     5761 gatgtacccg acccggaaca tgttgcctat accacccaaa cgaccctctc cgtgatggat
     5821 gccgcacgaa ttgtggaatg cctgcggcgg cggtttccta aacttgtcgg cccggcaagg
     5881 gacgacatat gctatgccac gcagaatcgt caggaggccg tcctggaatt ggcccgggaa
     5941 gctgacgtgt tcttggtcgt gggaagccaa aacagttcca acagccgtcg cttggccgag
     6001 attgcccaaa ggacgggtat ccccgcctat cttgttgatg gtccggagga tcttaaaaag
     6061 gagtggtttt cagcggaaca aacggtcgga atcacggcgg gggccagcgc cccggaacat
     6121 ctcgtgcaag gatgtattga gtttctccgt gcacattttt tcgccaccgt ggaggagcgt
     6181 gtcatccgca cagaaaacgt ccacttccag ttgcccccgg aacttcgcca agatcagcct
     6241 tgaggcgatc aatgcggcca gaccttgagc gtgacttcgc ccacttctag ttgagaaaac
     6301 gggcctgcta gtgtgggggc tgttcgcggc cgaccaaagt aatctgtatc gatgaccaag
     6361 ttccgcccat cgtagttctc ataaggaagc tgcgagacgg ctgtctgacc aagtcgggcg
     6421 gccgtgacga tttccctccc cgcgttagcc caatccgcag acgtcgtcag cgtgagaaaa
     6481 accataccct catcggccat gagccggcag aagggggctt ccttgatctc taaggagttt
     6541 gcctcgtttt gagcaggtgg cgcatgatcg aggtaaatat tgccctcggt cagattcatc
     6601 atggcctggg cggtgtaggc tgacagtcca aatcctgtga tccaggtccg tcgtggggca
     6661 ggggccccct ccggcatggg cgtgttttca ccccgaccga taaagacatt gttcaggaac
     6721 cgattgtccc caccctggat gctcgccaac ccggcgacct ctgttttgtg tggcggatgg
     6781 aacggcgttt tgcgattggg ttcggggcaa acggtgatcg cccccgcaaa cagattgtga
     6841 agataggcac caccttgtga catatcacgc agattcagtt tggaaaggaa cagattgtta
     6901 tccaccacaa agggaccatg gttgacttcc acgaacaggt cttcgtcgtt gtcatgaaag
     6961 agattggcgt ggacccgcgt tccttgggcc atccagtcaa gccacaatcc ccggcaggta
     7021 cggaaaatgt gattgcgggc aatcagcgta tcgatggcag catggaactt gatcccggcc
     7081 atttccgccc cggaaaagag tcgccgcatg tgaatgtcgt ggatcacatt gtcggtcact
     7141 tcgcaaaagg ccgcccccaa actgccgaca atcccggcct gttcgcaatg ggaaatgatg
     7201 ttgttacgga cgatgtggtg gccaatattg tctcgatgcc acccattggc cagggcccga
     7261 ttgatcgttt gcacatagcc ctcggccgta ttggctgacg tgttatcaaa ttcgtcgcca
     7321 tatttgccaa gtgtgattcc acagcaaatg gagtggctaa tgacgttgtt ctcgatgatc
     7381 caacctttgc tccagtgcgt accgatcagc ccgatttgtt gggctgtggg cggtgcccag
     7441 ggtgtggccg catgacgcat gataaaacca cgaacggtga tgtaattgca ccctggcgtt
     7501 cgtggataaa agacggtttg ccggacgttg atctccacgg tttctcggtt cggatcgacc
     7561 ccggggaact gcgcccaaat cgtggtcttt tcctcatcaa cctcggcaaa ccaccatcgc
     7621 aaacccgacg agggcttgaa aaggtcctcc ttactggcag cctcggcaag ccattctcca
     7681 ttgaggtaca ccgcccccgt gtgatgcacc cgtccccgcg ggctaaacca atctcctttg
     7741 atcacgtcgg cataaggatt gaagttccca aaaaacgtgt tggggatcac gacctgccag
     7801 acatcgcctt ctaccttggt ccaccccttg atgacttccg acccacggat ttcgacctgt
     7861 tcattcgggg ccgcttggta ggtaatacgt tgctcatcgg aaaggcctcc ccggggcggc
     7921 gccacctcct cgcggtagat tccggcatgg acaattaccg tatcgccagg ctgggcaagt
     7981 tgggcagccg ccgagattgt tcgcagggga cgttctggcg tcccgtcgtt ggaatcgttg
     8041 ccgcgaagcg cgacatgaat ctctcggcaa aaacccgg
//
LOCUS       DAOSUT010000176         8008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4601_length_8008_cov_7.830378, whole genome shotgun sequence.
ACCESSION   DAOSUT010000176 DAOSUT010000000
VERSION     DAOSUT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_8008_cov_7.830378"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1735
                     /locus_tag="PLD05_13370"
     CDS             293..1735
                     /locus_tag="PLD05_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78479.1"
                     /translation="MKRYIDALFPVVCLILGGVYFMARYRHEGSVGLAAVMALAIFVL
                     AAFVSYAFSYTVLAPGRKVWRLIGFVGLVPCLFGFGEALSRHMWRGLVSLESLDSPRP
                     RYICWDSAGQTSIAPITYPLKEFHRKGDETAHLAAAMAIRKTAGVPHRIAELIDKPEG
                     HDRSYWLRRALTGHPPGLAIIYSPVAENPPLARIWAGLIFLLCVGAAFWAGNQWSPDS
                     HFGLLTAACFASIPNLDWWHAVSVSSDIPPCFFSFVGFGLLAPAIMRDKWESDEGHRI
                     KLAGLLLAVACFVTLTASLASLAAAVLMVSTKGRQGICYAAWLLIPSVISVALGIAYS
                     RLAVPDAAHVVLARLETVGESGPTSFTDPLRGLLTFVRRCPMDLGIPLTLAFILLPVR
                     QIVSLAHGGSRRWIFGGFCAAIAVLAPAAMMFWPEIRFAYPGFLAVMLGLGFLDFWVT
                     VSPRTRALTIAAIAGFAYSKFVLHNLLVVG"
     gene            complement(1910..2161)
                     /locus_tag="PLD05_13375"
     CDS             complement(1910..2161)
                     /locus_tag="PLD05_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78480.1"
                     /translation="MCLILLLTRALGRKQFGIFATALALQGYIVLLATAGMPAVCIVL
                     ALSMAVWDAYLHDDLAARAWLPGNRPKSVLDSRDRWQRK"
     gene            2285..3424
                     /gene="rffA"
                     /locus_tag="PLD05_13380"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             2285..3424
                     /gene="rffA"
                     /locus_tag="PLD05_13380"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOP78481.1"
                     /translation="MIPFNKPFIAGKELYYIAQAVTMGNIAADGYFTKACSRLLEERF
                     GIRKVLLVPSGTAALELAAMLCNLQPGDEVIMPSYTFVSTASAFVRVGARPVFVDIRP
                     DTLNIDETKIEAAITERTRVIVPVHYGGVSCEMDRIMDIARRYNLLVVEDAAQGVNAF
                     YKGRALGSIGHLGCYSFHETKNYICGEGGALCINDERFIERAEILRDKGTNRQKFFRG
                     EVDKYTWVDIGSSYVMAEILAAFLYGQLEQMDAIAARRFEIYQHYAMHLKPLEEQGFL
                     RLPRIPAGCQSNHHLFYVLTPDGPTRDALLAHLKSEGIHAVFHYVPLHTSPMGRTFGY
                     KEGDLPVTESLAARLVRLPMYYELTQHDQERVVVTLEKLFSEILI"
     gene            3475..4212
                     /locus_tag="PLD05_13385"
     CDS             3475..4212
                     /locus_tag="PLD05_13385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP78482.1"
                     /translation="MLDWDSRHFGFAVAKANWSGEGSDLQRLINWAVDKQVTLLYLFA
                     PTSVVLPTWTTQRCVGVFVSERVEFEKAVGSNRDADFGAEVAIMELTAENCQLDSVTR
                     LGVLAGRFSRFSRDPRIPQGKAQKLFEIWAEKSVRRELADTVFGAFRADGDLIGFVTL
                     KQDGLIGRIGLLTVVPERRSMGCGRALVCATETWAAARKLTTLRVVTQADNEAACGFY
                     RRVGFHECERQVVFHLWLREPSNGTRH"
     gene            4176..5144
                     /locus_tag="PLD05_13390"
     CDS             4176..5144
                     /locus_tag="PLD05_13390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP78483.1"
                     /translation="MAPRTEQRYETLNTDLSIVIPVFRGAATIASVVEGVIDCLRAAG
                     MSGEIILVDDGSPDETWQVLKDLHAEFPNIVRAIRLMRNFGQHNAIMCGLRHAQGNII
                     VTMDDDGQHPPEELPKLIRAIEESGADVVYGVPKERSHAPWRNLGSWLVVTFYQWVFR
                     TNVTPSAFRIMRRQVVDAILTYDLNYTYVDGLLAWNTDRIAQLEVEHRPRQHGRSGYN
                     LGKLLTLAMNLFTNFSILPLQIVSIMGFVVAVLGFLLGLYYILQRLLGGIAVPGYASI
                     IVAILVLGGLQMLSVGIIGEYLGRVHININRKPQYTIREILTQGEP"
     gene            5146..6369
                     /locus_tag="PLD05_13395"
     CDS             5146..6369
                     /locus_tag="PLD05_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78484.1"
                     /translation="MIGPGLPHIPLSVSTLVPYLAIGPITLAIFAPLYYEMARGTISS
                     DIPAHAEIVKRVTEGGRWPPHPGLHGLALVLLGFPNSLNWKSTILVLSGITLGSLLVK
                     HLLAATYLASWASFPSANNRESISASCTDASPAGWTLVGLTMCLCLAGPIWSPEIGWQ
                     NFYLGKFSPNAWHNPTLLLSWPFIILHFAGLLQCLNQPNFKRLCQVLFYLALATIIKP
                     NYAIAFCGALPITLLLTSFRSPAWTRAVILQLAVAVLVLLPQFAAFQAEENAVVVLAP
                     FTIVSQRSGFIPLTFLTSVAFPLIVAITLRRGLLGNQAFQLAALLFLVAAAQCYLLVE
                     SGPGRSALHGNWYWGAHATLFLLFLVSLAALWRATFGEARRDFGIYACWGVFGLHVVT
                     GVLWLTKHFIACSYY"
     gene            6481..>6734
                     /locus_tag="PLD05_13400"
     CDS             6481..>6734
                     /locus_tag="PLD05_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78485.1"
                     /translation="MGHRTFPILGLVASLAFVPILLWGQFLFPIGDDLESANWPGGWH
                     ALYQVPFTLWHTWSGCYSASFFRVLHAPLTRAGVFWTTPL"
     assembly_gap    6735..6834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6835..7559
                     /locus_tag="PLD05_13405"
     CDS             <6835..7559
                     /locus_tag="PLD05_13405"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOP78486.1"
                     /translation="GAPGNFQRYVFRMSDAPITFGYVVTRSIGSFVEFLIRELSLVQN
                     WLWVTFGTIFVSRHVVKENNRSTLVGVIVRVLAVLLPIFGIFVLSTMTREVPPPARTL
                     NALHYLLMLGVIVVVAPVVLKLSWVSARLHRWLDDSHVFRKAAAATLCFAVLSPNYVR
                     CVRELVTVAPQYKQYWYEVERRVAEAKEKGLPRVEIPSDPERRPVTVFHPSFLGPDPE
                     AWPNRDYAKFFGIAQIVAVETK"
     gene            7650..>8008
                     /locus_tag="PLD05_13410"
     CDS             7650..>8008
                     /locus_tag="PLD05_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78487.1"
                     /translation="METQTGNQHRTMRICVVTPQPTPYRDPFWNAVAAQPGVELDVFY
                     CYAKGDDRPWEIDWPFQFHAEVLPGRAWLGQHNGYWNPAILKRLRAKRYDAILLGGYN
                     HFTMLAAAWYGRKRGIP"
BASE COUNT         1642 a         1976 c         2221 g         2069 t
ORIGIN      
        1 ccgacccgca ggcgtaaaaa acgattttcc gaaagaaacc aaactccagt tgcgtcccat
       61 aaggcgttgc aatccctccg gagcaagagc atgtccttca attatggcct atagttttgg
      121 ggcatacgat tgccggcggc aggtaagggg gtaattcgcg tgggaaaacc ggagtaaagc
      181 aagcggaggc gttgaaatag ggtacctgcg gcattgtgtg aggaattagc gttcgcaaaa
      241 cgatgccgag aaggtacgca aagctaaatg tttcggttac cgggtaaacg agatgaaacg
      301 atatattgat gcactgtttc ccgttgtgtg cctgattctt gggggcgttt acttcatggc
      361 gagataccgc cacgagggaa gcgtgggact ggcggctgtc atggccttag ccatatttgt
      421 gttggctgct tttgtcagct acgccttctc ctacacagtg ttagcgcccg gacgcaaggt
      481 gtggcgtctt attgggttcg ttggtcttgt gccttgcctt tttgggttcg gcgaggcact
      541 tagtcgccac atgtggcgcg ggctcgtgag tttagaatcg ttggactcgc cgcgcccgcg
      601 gtatatttgc tgggacagcg cagggcaaac aagcatagcc cctatcacct acccgctaaa
      661 ggagtttcat cgtaaagggg acgaaacggc tcatttagca gcagcgatgg cgatccggaa
      721 aactgcaggt gtaccccacc gcattgccga gctgatagac aaaccagagg gtcacgatcg
      781 gtcctattgg ttaagaaggg cgttaacagg acaccctccc ggcctggcaa ttatctactc
      841 gccggttgct gaaaatccgc cccttgcacg gatctgggca ggcctcattt ttctgctatg
      901 cgtgggggcg gcgttctggg cgggtaacca gtggtcgccc gattcacatt tcggtctgtt
      961 aactgccgca tgtttcgcgt cgattccaaa tttggattgg tggcacgctg tttcggtctc
     1021 ctcagacatc cccccgtgct tcttctcatt tgtggggttt ggattgctgg ccccggccat
     1081 tatgcgcgac aagtgggagt cggacgaagg gcatcgcatt aagctggccg ggctgttact
     1141 cgccgtggcg tgttttgtga ctctaacggc gtcattggcg tcgctggctg cggccgttct
     1201 catggttagt acaaaagggc gtcagggaat ctgttacgca gcgtggctgc tgatcccaag
     1261 cgttatatcc gtggccttgg ggattgctta ctccagactc gccgtcccgg acgcagctca
     1321 tgtggtgctt gcgcgtctgg agacggtcgg ggaaagtggg ccgaccagct tcactgaccc
     1381 gttgaggggc cttctcacat tcgtgcgtcg gtgtcctatg gatctaggta tccccttgac
     1441 cttggcattc atcctactgc cggtacggca aatcgtctcg ttggcacatg gcgggtcacg
     1501 ccgctggatc tttggtggat tttgtgctgc gatagcggtc ttagctcccg ccgccatgat
     1561 gttttggcca gagattcgtt ttgcatatcc tggattctta gcggtcatgc ttggtctggg
     1621 cttcctagat ttttgggtaa ctgtctcacc tcgcaccaga gccctcacca tagctgccat
     1681 agcaggtttt gcgtattcca agttcgtact acacaatctg cttgtggtgg ggtgatgtgg
     1741 tccgcactat ggcactgttg cggagatttg attcaaaact aactgtggtg tgatgctgct
     1801 tcaatttggc gtttcactcg gctttatttc tccgtgagcc aacgtcatgt ggcggctgag
     1861 actgtaactc gttgatgttg tctatggttg agtcggacaa atccgagaat tattttctct
     1921 gccaccgatc acggctgtcc aacactgatt tcggtcggtt gccgggcaac catgcccggg
     1981 ctgcgagatc gtcgtgaaga tatgcatccc agacagccat gctgagcgcc aggacgatgc
     2041 aaacggccgg catcccagcc gtggccaaca ggacaatgta accctgaagg gcgagtgccg
     2101 tagcgaaaat gccgaactgc ttgcgtccca aggcccgggt gagaaggaga atcaggcaca
     2161 ccagccccca accgggttag caatcccggt accatgaggt agctaccgcg cagcataaac
     2221 tgtttgcaat gtaacatgaa ttgttttacc gaagttactt tcgtcagaac acacggcatc
     2281 ccccatgatc cccttcaaca aaccgtttat tgccgggaag gaactttatt acattgccca
     2341 ggcggtcacg atgggtaata tagcggccga tgggtatttc accaaggcgt gttctcgctt
     2401 gttggaagag cggttcggca ttcgcaaagt gctcctcgtc ccgtcgggta cggcggcctt
     2461 agagctggcg gccatgctgt gcaaccttca gcccggcgac gaggtcatta tgccgtcgta
     2521 cacgtttgtt tccactgcca gtgcctttgt gcgagtgggc gcccgacccg tgttcgtgga
     2581 tattcgtcct gatactctca atattgacga gacgaagatc gaggcagcca tcacggaacg
     2641 aacgagagtg atcgtgccag tgcattacgg gggagttagc tgcgaaatgg accgcatcat
     2701 ggatatcgcc cgcaggtata acctgctggt ggtagaggat gccgcccaag gcgtgaatgc
     2761 gttttataag ggtcgcgccc tcggatctat tggccatttg ggttgttaca gcttccatga
     2821 gacgaagaac tatatttgcg gagaaggcgg ggcgctgtgt atcaacgatg agcgattcat
     2881 cgaacgggca gagatccttc gcgacaaggg cactaatcgt cagaaattct tccgcggcga
     2941 ggtggacaag tacacgtggg tcgacatagg atcgtcctac gtgatggccg aaatcctggc
     3001 cgcctttctg tatgggcagc tcgagcaaat ggatgcaatc gcggctcgcc ggtttgagat
     3061 ttaccagcat tacgcgatgc atctcaagcc tctcgaggag caaggcttct tgcgtctgcc
     3121 gcgaatcccg gctgggtgtc aaagcaatca ccatctgttt tatgtgctta caccggacgg
     3181 gccaactcgg gacgctttac tcgcccattt gaaatcggag ggtattcacg cggtgtttca
     3241 ctatgtaccg ctgcacacct cgccgatggg ccgaacgttc ggttataaag aaggtgatct
     3301 gccggtgacg gaaagtcttg cggcccgttt ggtccgcttg cccatgtatt acgagttgac
     3361 acaacacgat caggaaaggg tcgtcgtcac cctagaaaag ctttttagcg agatactgat
     3421 atagtcgcag tggtcgagaa tagagtaaat gcgactgata cgattaaccc gattgtgtta
     3481 gattgggaca gtcgtcattt tggctttgcc gttgcaaagg cgaactggtc aggagaggga
     3541 agtgacctcc aacggctgat taattgggct gtagataagc aggtgacgct tttatatctt
     3601 tttgctccaa cttcagttgt tttgcctacc tggaccaccc aacgctgcgt gggtgtcttt
     3661 gtgtctgaac gtgtcgagtt tgaaaaagcc gttgggtcca accgcgacgc cgattttggg
     3721 gctgaagtag ccatcatgga attgaccgcc gagaactgcc agttggactc ggtaactcgg
     3781 ctgggggtgc ttgcagggcg attttcaagg tttagtcgtg acccgcgaat cccacagggg
     3841 aaagctcaga agctctttga aatctgggcc gagaagagcg tacgaagaga gttagctgac
     3901 accgtgtttg gggctttccg tgctgacggg gatttaatag gattcgtcac tctgaagcaa
     3961 gacggtctga ttgggcgaat cggcttgctc acggttgtac cagagagacg gagcatgggc
     4021 tgcggacgag cattggtttg tgccacggaa acctgggcag cggcacgaaa gcttaccacg
     4081 ctacgcgtcg tcacacaagc tgataatgaa gctgcttgtg gtttctatcg gcgagtcggg
     4141 ttccacgaat gcgaacgcca ggtggttttt cacctgtggc tccgcgaacc gagcaacggt
     4201 acgagacatt gaacacggat ctttccattg tcattccggt ctttcgcggt gctgccacga
     4261 ttgcgtcggt cgtggaaggg gtaatagatt gtttgcgagc cgccggtatg tctggtgaga
     4321 tcattctggt ggacgacggt agccccgatg agacgtggca ggttttgaag gatttgcatg
     4381 cagaattccc gaacattgtg cgagcaatcc gcctaatgcg gaacttcggc cagcataatg
     4441 cgattatgtg cggcctccgg catgcccaag ggaatattat cgtcacgatg gacgacgatg
     4501 gacaacatcc gccggaggag ctacccaagc tcataagggc catcgaggag agcggcgcgg
     4561 acgtggttta tggggttcct aaggaacgta gccatgctcc gtggcggaat ttaggatcgt
     4621 ggttggtcgt gactttttat caatgggtat ttcggacaaa cgttacgcct agtgctttcc
     4681 gtatcatgcg gcggcaggtt gtggatgcga tccttactta tgatttgaat tacacatacg
     4741 tcgatgggct tctggcatgg aatacggacc ggatagcaca actcgaggtt gaacatcggc
     4801 cacgacaaca tgggcgatcg ggatacaacc tcggcaagtt gttgacactg gcgatgaacc
     4861 tgtttacaaa cttttccatt ctgccgctgc aaattgtttc gatcatgggc tttgtggtag
     4921 cggtgttggg tttcttgctc ggtctttact acatcctcca gcggcttttg ggaggaattg
     4981 ccgtccccgg gtatgcatca atcattgtgg ccattttagt gctgggtgga ctgcaaatgt
     5041 tatccgtggg gattatcggg gaatacctgg gccgcgtgca tattaacatc aaccgcaaac
     5101 cgcaatacac aatccgggag atattgacac agggggaacc gtgagatgat tgggccaggc
     5161 ttaccgcaca tccctctgtc agtatcgaca cttgtcccct atctcgccat tgggcctatt
     5221 acgttggcaa tttttgcacc cctatattat gagatggccc gtgggacaat tagttcggat
     5281 attcccgccc atgcggaaat tgtgaaaagg gtaacggaag gcgggcgttg gccaccccat
     5341 ccgggcttgc atggtctagc ccttgtatta cttggattcc ctaattcgct taactggaaa
     5401 agtacgatcc tcgttctctc agggatcacc cttgggtcac ttctcgtgaa acaccttctg
     5461 gcggccacct atctcgcgtc ctgggcatca tttccttccg ctaacaaccg agaatcaatt
     5521 tcagcgtcat gcaccgacgc gagtcctgcg ggatggacac tcgtcgggct aactatgtgc
     5581 ctttgtctcg ctggaccaat ttggtccccg gagatagggt ggcagaactt ctatctgggc
     5641 aagttctcgc cgaatgcctg gcacaatccc acgcttttgt tgtcgtggcc attcatcatc
     5701 ctgcattttg ccggtttgct gcaatgcctg aaccaaccta actttaaacg gctttgccag
     5761 gtccttttct acctggccct tgctaccatt atcaagccga actacgcgat tgccttctgt
     5821 ggggcactcc ctatcacgct ccttcttacg tcgttccggt ccccggcctg gactcgggcc
     5881 gtgatcctcc aactcgctgt tgccgtgttg gtcctactcc ctcagttcgc tgctttccag
     5941 gcagaagaaa acgccgttgt ggtactcgca ccctttacta tagtgtctca acgaagtgga
     6001 tttattccgt tgacattctt gacttcggtt gcttttcctc taattgtcgc tattaccctg
     6061 cgtcgaggtc tccttggaaa tcaggccttt cagctagcgg cactattgtt tttggtagct
     6121 gccgcgcaat gttatctttt agtagaaagc ggacctggcc ggagtgcgtt gcacggcaat
     6181 tggtactggg gcgcccatgc aacgttgttc ctcctttttc tggtgtcact tgcggcattg
     6241 tggcgagcca ccttcggcga ggcacgccgc gatttcggca tttacgcctg ttggggtgtt
     6301 ttcggtctcc acgtggtaac gggggtactg tggttgacga agcattttat tgcttgtagt
     6361 tattattaaa acggcatcta tctgcagaac aacaaaacgg cgcaatcccg tagaagtatt
     6421 tcataatagc ctgttatcaa agcattgaga ccactgcatt ttcaaacgtc ggatcttgac
     6481 ttgggtcatc ggacttttcc tattctggga ttggtagcga gtctagcctt cgtaccaatt
     6541 cttctttggg gacaattcct ttttcccatc ggggacgatc tggaaagtgc gaactggccc
     6601 ggtggctggc acgccctgta ccaggttcct tttactctat ggcatacctg gagcggctgc
     6661 tattcggcat cgttctttcg agtactgcac gctcctttga cacgagcggg tgttttctgg
     6721 acaacaccgc tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttggtg
     6841 cgccaggaaa ctttcaaagg tatgtgttca ggatgagtga cgcgccgatc acgttcggtt
     6901 atgttgtgac tcgctcgatc ggctctttcg tcgagttttt gattcgcgag ttgagtcttg
     6961 tgcaaaactg gttgtgggta acttttggga caatatttgt gtcgcgtcac gttgtcaagg
     7021 aaaacaatcg ttcgacgctg gtaggtgtca tcgtgcgagt tttagctgtc ctgctaccga
     7081 tttttggaat cttcgttttg tcaacgatga ctcgtgaagt acctcctccg gcacgcacgt
     7141 tgaatgcctt gcattattta ctaatgctgg gagtcatcgt ggtcgttgcc cctgtggtgc
     7201 tgaagctatc ctgggtatcc gcgcgtcttc acaggtggct cgatgattcc catgtttttc
     7261 gcaaggccgc ggcggcaact ctgtgttttg cagtcttgtc gccaaattat gtgcggtgcg
     7321 ttcgtgagtt agtcacagtc gcacctcagt acaagcagta ctggtatgag gtcgaaagac
     7381 gtgttgcgga ggccaaggaa aaggggctgc ctcgagtcga aataccttcc gatccagaaa
     7441 ggaggcctgt aacggttttt catccgtcct tcctcggtcc tgatccagaa gcatggccca
     7501 acagagatta tgctaagttt ttcgggatag cccagattgt agccgtcgaa acgaaatgac
     7561 aagaaatcga acgctttgac ttgttgagtc gcggctattc cagtaacgcg cgattgtcgg
     7621 aaagagccac aacgcaaaat actgttaata tggaaacaca aacgggcaat caacaccgaa
     7681 cgatgcgaat ttgcgttgtc actcctcagc cgacaccgta ccgtgaccca ttttggaatg
     7741 ccgtggctgc gcagcccggg gtcgagttag atgtttttta ctgttatgcc aaaggtgatg
     7801 atcggccatg ggagatcgat tggccgtttc agttccacgc tgaggttctt cccggccgtg
     7861 cgtggttggg acagcataac ggttattgga atccagcaat cttgaagagg cttcgcgcaa
     7921 agcgttacga tgccattctc ttggggggct acaatcattt cacgatgctt gccgctgcat
     7981 ggtacggccg aaaacggggg ataccata
//
LOCUS       DAOSUT010000177         7996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4608_length_7996_cov_9.053520, whole genome shotgun sequence.
ACCESSION   DAOSUT010000177 DAOSUT010000000
VERSION     DAOSUT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_7996_cov_9.053520"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLD05_13415"
     CDS             <1..257
                     /locus_tag="PLD05_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78488.1"
                     /translation="GPVNSAAFSPNGRRIVTASEDKRAIVWDATSGQKLLVLEGHTGM
                     VVSATFSPDSRRILTASLDNTAIVWDATSGRKLLVLRVSE"
     gene            359..1618
                     /locus_tag="PLD05_13420"
     CDS             359..1618
                     /locus_tag="PLD05_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78489.1"
                     /translation="MQSKNSLLRRGASQVVTAFLVLTIVALLGVIVMLLIGRESPQTA
                     DNRAAAKSEQVSPKSGESAAAPQEPNVSPPTKSSTPPSPPKPAPPPPPKPVGDPERIR
                     DVLQQGKTYESTLKVGFQAAVIDADWGVRQTISLVYEAENRMRRTVERNDGQTVVLLC
                     HVVESRAVKLLTDVEVTFDLGQPGTLLLGALDYWLTGGQLTAPAVVAAPVAEAILTEA
                     SRAALKGHTAKAKAMLDSLAGKRFRVTYIDGKGLVSLEPVGCTLTEEERDYLEGMSVL
                     GDCYLLPDVNSRPGQYWDVEARELVEFLPPTWRGRPSGVVTIERGQDFKKGDKDYATL
                     KIRTGTFQVNASDRSRRRLADVTPRGELYYNITDGYIDRAELRARASVEDVSTDHLLF
                     ESRFQTAPELEIQYTCQRLEGPMGSGE"
     gene            1622..3712
                     /gene="ccmA"
                     /locus_tag="PLD05_13425"
     CDS             1622..3712
                     /gene="ccmA"
                     /locus_tag="PLD05_13425"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOP78490.1"
                     /translation="MSKWTEESRVVPFTSLPLSVGRAPDCDLCLNEPTVSRRHALIFR
                     RKDDIVVSDQGSLFGTFVNGQRVREWVLRRDDRVRFGARISYVVQENGLQREGLGVAA
                     LQVRNVSIARQGRMLVEGVSAVFTTGNLVGILGPSGLGKSLLLRVLAGYNQPEEGQVV
                     WDGYDVWADREGYLTQVGFIPQADVLYETLSVRENLEYAARLRLPGLSESARRERIEE
                     VLDLLQLKEHAEKRAVVLSGGQRKRLSVAIELLRKPPILLLDEPTTGLDPGNELRLME
                     NLRQVASRGTLVVLSTHSLAALSLIDQVLVLARYGGVGRVAFCGPVETLRSRLAGMHP
                     ADWYNALEQGRVAPKLPGGQRPPDGTHGHVATATQGDLLDSWAERFPPPLVLPLRRIA
                     RRQGGAAWWHQWVDVAVRCAKGIVRDRALVAMMAVQPVLLALLVILSQGRASKIDPIL
                     FFTVVVAIWLGMNNTVRDLVRERRQYLRDRMAGLQAEAYLAAKVSLYLAIGIIQLLVF
                     FLLVRLGCSQAIDESLAKKLSETGDLGWLTTLSLVYLCGVGLGMLVSTLSRTEEAAVA
                     SLPILILPQILLSAVATGDVYERFSDPRICRPIVVTLNPGLGEAREADRDRQKSAFGQ
                     LGWAGTVTDILSMCCYSRPALLVLQRPKVPGYSANIWLADLFHLLLLLCATHGAMWGL
                     FLKYESRWPTFVAL"
     gene            complement(3881..4942)
                     /gene="aroF"
                     /locus_tag="PLD05_13430"
     CDS             complement(3881..4942)
                     /gene="aroF"
                     /locus_tag="PLD05_13430"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOP78491.1"
                     /translation="MIVVMKRGASQSQIEYVVDRVQAFGLRAHVIYGTERTVIAAIGD
                     KRDEHRASLESCPGVAEVIPILAPYKVASLEVNPEHTVVRVGPFVCGNSHIGVIAGPC
                     SVETEEQTLSTARAVKAAGATGLRGGAFKPRTSPYSFQGLKEEGLKILALARQETGLP
                     VVTEVITPSDVELVAQYADILQIGARNMQNYRLLEEAGQAGKPVLLKRGPAATAEELL
                     LAAEYVLNTGNHNVILCERGIRTFESHTRYTLSLATVVWLHQRTHLPVIVDPSHGTGH
                     TALVPQMAAAAVAAGADGLIIEVHPRPEAALSDGYQSLTFEQFRETMDLCCKVAHALG
                     RHLGSRALPTATQLVGAAC"
     gene            5470..5949
                     /gene="bcp"
                     /locus_tag="PLD05_13435"
     CDS             5470..5949
                     /gene="bcp"
                     /locus_tag="PLD05_13435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOP78492.1"
                     /translation="MSDWVEVGQPAPDFTLPSDSGEHVRLSQFRGSPVVLYFYPKDDT
                     PGCTREACAFRDLKQELVKLGAVILGVSPDGVESHKKFREKFNLNFPLLADVDHSVAE
                     AYGAWREKTRFGKKSMGIQRSTFLIDANGVVRKVWKNVQVDGHDDQVLEALKALTQE"
     gene            6036..6782
                     /locus_tag="PLD05_13440"
     CDS             6036..6782
                     /locus_tag="PLD05_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78493.1"
                     /translation="MKRFWFILVNCGAIVLSAGLGCRNEREANVTVLEPQKTIIVRAE
                     NTAQPPLPAQPSGEQPNAPTIIPQPGDSVGNAKPEITGATANNRQIVLGGRFRATVPA
                     HWVAKPPAISLIEHEFAVPSTIEGEPDGRVTFSSARGGVQANIERWANQFRFAAGQDL
                     RQAVKHEKTSILGENLHLVDISGTYLETFGPRMEKAVEREGYRMLGAIIECQDGFLYF
                     IKFYGPQATVKENELAFRAMIESLQAVGPQ"
     gene            6916..6986
                     /locus_tag="PLD05_13445"
     tRNA            6916..6986
                     /locus_tag="PLD05_13445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6948..6950,aa:Gln,seq:ctg)
     gene            7770..>7996
                     /locus_tag="PLD05_13450"
     CDS             7770..>7996
                     /locus_tag="PLD05_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78494.1"
                     /translation="MIRYICDLCRREIDSTREVCYHVEIQISAAFEPVTEDEPDDRDH
                     LEEIHDLLESLNQLGDEAYESAHETLRFDLCP"
BASE COUNT         1699 a         2118 c         2413 g         1766 t
ORIGIN      
        1 ccggccccgt taattctgcc gcgtttagtc cgaatggccg gcggatcgta accgcgtcgg
       61 aggacaagag ggcgattgtg tgggatgcca caagtggcca aaagctcttg gtgctcgaag
      121 ggcacaccgg catggttgtg tctgctacgt ttagtcccga cagccggcgg attttgaccg
      181 cgtcgttgga taacacggcc atcgtgtggg atgccaccag tggccggaag cttttggtat
      241 taagggtgtc agagtgaccg gcgttgttgc cgccagcggg gcccaccaaa gggaccgcgg
      301 cggctggaaa ggtgaggttc gtagtgagat ggtgtggttt taccagtagg aggctcgtat
      361 gcaatcgaaa aattcgcttc tgcggcgtgg cgccagccag gtggtgaccg cattcttggt
      421 cttaaccatt gtggcgctac taggcgtgat tgtaatgttg ttgattgggc gtgagtcgcc
      481 ccaaactgct gacaataggg cggcggctaa gtcagagcaa gtgtcgccta agtcaggcga
      541 gagtgccgct gccccgcaag agcctaacgt ctctccgccg accaagtcat ctacgcctcc
      601 ctcgccgccc aagccggctc cgcctccgcc gcccaagcca gtgggggatc ccgaacgtat
      661 ccgagacgtc cttcaacagg gtaagacata cgagagcacg ctcaaggtcg gcttccaggc
      721 cgcggtcatc gatgccgatt ggggtgtcag acagaccata tccctcgttt acgaggccga
      781 gaaccgtatg cggcgtacgg tggagcgaaa cgacggccaa acagtggtcc tgctgtgtca
      841 cgtggtagag agtcgggcgg tgaaattgct cacggatgtc gaggtgactt ttgacctggg
      901 ccagcccggg acgcttctcc taggggcgct ggactactgg cttacgggtg ggcagcttac
      961 tgccccggcg gtggttgcgg ctccagtagc ggaagccatt ttgaccgaag ccagccgcgc
     1021 ggcgttgaaa gggcacacgg ccaaggccaa agcaatgctc gattccctgg ccggcaagcg
     1081 tttccgtgtc acctatatcg acggcaaggg cctggtgagc ctggagcccg tgggatgcac
     1141 cttgacagaa gaagagcgtg actacctgga aggtatgtcg gtgctgggag attgttactt
     1201 gttgccggac gtcaacagcc ggccgggcca atactgggat gtcgaggcga gggaactggt
     1261 agaattcttg cccccgacgt ggcgaggtcg gccctcaggg gtggtcacta tcgaacgggg
     1321 ccaggatttc aagaaaggag acaaggacta tgccacgctg aaaatccgga ctgggacatt
     1381 tcaagtaaac gcctcggacc gaagtcgccg gcgattagcc gatgtcaccc cacggggtga
     1441 attgtattac aacattacgg atggatatat cgaccgcgcc gagctacggg cgcgggcttc
     1501 ggtagaagac gtatcgaccg atcatttgtt gttcgaatcg cgattccaaa cggcaccgga
     1561 gctggaaatt cagtacactt gccaacgatt agagggacca atgggctcgg gggaataaac
     1621 gatgtctaag tggacagagg agtcacgggt ggtgccgttc acctccttgc cgttgtcggt
     1681 cggtcgagca cccgactgtg atttatgttt gaacgagccc acggtgtcgc gccgtcatgc
     1741 gctaattttc cggcgaaagg atgacatagt cgtatcggac cagggcagtc tgttcggaac
     1801 ctttgtcaat ggacagcggg tgcgcgagtg ggttctccga cgggatgatc gcgtgcgatt
     1861 tggtgcgcgg ataagttacg tggtgcagga aaatggattg cagcgcgaag gcctgggcgt
     1921 ggcggccctg caagtgcgaa atgtaagcat tgcacgacag gggagaatgc tggtcgaggg
     1981 agtctcggcg gtcttcacga cgggcaatct ggtggggatc ctcggaccga gcggactggg
     2041 caagtcactc ctattacgcg tgctggctgg gtataaccag ccggaggaag ggcaagtggt
     2101 ttgggatggg tacgatgtgt gggcggaccg tgaggggtat ttaacgcagg tcgggtttat
     2161 tccgcaagcg gatgtgcttt atgaaacact ttctgtccgt gagaacctag aatacgctgc
     2221 ccgactccgt cttcccggcc tttcggaatc cgcccggagg gaacgcattg aggaggtgct
     2281 ggatctactt caactgaagg agcacgccga gaagcgcgcg gtggtgctta gcggagggca
     2341 gcgcaagcgg ctaagtgtgg ccattgagct gctgcgcaag ccgccaattc tgttacttga
     2401 tgagccgacg acaggtttgg atccgggaaa cgagttgcgg ttaatggaaa acctgcggca
     2461 ggtggcaagt cgcggcacgt tggttgtgtt gtcaacccat tcgcttgcgg cgctgtcctt
     2521 aatcgaccag gttttggtgt tggcgcggta cgggggggtg ggccgggtgg ccttttgcgg
     2581 gcctgtcgag acgctccgga gtcgccttgc aggcatgcat ccagccgact ggtataacgc
     2641 gttggaacag ggccgggtgg cgcccaagct cccaggggga caacgacctc cggacggcac
     2701 acatggtcac gtggcgacgg cgacgcaggg cgacctcttg gactcctggg ccgagcgatt
     2761 cccaccgccg ctggtgcttc ccctgcgacg aattgcgcgc cgccaaggag gcgctgcctg
     2821 gtggcaccag tgggtggatg tggccgttcg gtgtgccaag ggcatcgtta gggatcgggc
     2881 cttggtggcc atgatggcgg tccagccagt tctgctagcg ctgttggtga tcctttcgca
     2941 aggtcgcgcc tccaagatag accccatctt gttttttacg gtggttgtgg ccatttggct
     3001 tggtatgaac aatacggtgc gggaccttgt tcgcgagcga cggcagtatc tccgtgatcg
     3061 catggcaggc ttacaggccg aggcgtacct ggcggctaag gtaagccttt acctggccat
     3121 cggcataatc cagctcctgg tctttttctt gttagtgaga ttgggctgtt cacaagcgat
     3181 cgacgaaagc ctcgccaaga agctgagtga gacaggtgac ctaggctggt tgacaacctt
     3241 gagcctggtg tacctgtgtg gtgttgggct aggtatgttg gtgtccacgt tgtcgcggac
     3301 ggaagaggcg gcagtggcca gcctaccgat actcatcctt ccccaaatct tactgagcgc
     3361 ggtcgccacg ggcgatgtgt acgaacggtt ttcggacccc cgtatatgcc ggccgattgt
     3421 cgtcacgctg aaccccggcc tgggtgaagc gcgcgaagcg gacagggaca ggcagaagag
     3481 cgcgtttggg caactaggct gggcaggcac tgtgaccgac atattgtcga tgtgctgcta
     3541 tagtcgcccc gcattgctag tgctccagcg tccgaaggtg ccgggatact cggcgaacat
     3601 ctggttggcg gacctcttcc atttgctcct gctgctttgc gccacgcacg gcgccatgtg
     3661 ggggcttttc ctcaagtacg agagccgctg gcctacgttt gtggcattgt aataagcggt
     3721 agcgtcttgt ttgatgtctt cacgtccgga acccttggca ctgcgatttg cgtcgttgcc
     3781 ggacaaacgg tccataggca cgtacgatcg agccgcttgc cttgtgaagc accacttcga
     3841 tgcagtggca catcgtgtgc cgaaacttga cacacctacg ttagcaagcg gcaccaacta
     3901 gttgggtggc ggtgggcagg gccctagagc cgagatgtcg accgagggca tgagccacct
     3961 tacaacacag gtccatcgtc tccctgaatt gctcaaaggt gagggattga taaccgtcgc
     4021 tgagagccgc ctcaggccga ggatgaacct caatgatgag tccatccgcc cctgctgcta
     4081 cggccgcggc ggccatctgt ggtacgaggg cggtatgtcc cgtcccgtgg ctgggatcca
     4141 caatgacggg caaatgcgtt cgctgatgaa gccaaacgac agtcgccaaa gaaagtgtgt
     4201 aacgtgtatg cgattcgaac gttcggattc ccctttcgca caggatcacg ttgtggttgc
     4261 cggtgttgag cacatattcg gcggcgagga gaagttcttc tgcggtggcc gccggccctc
     4321 gcttcaacag gacgggtttg cccgcctgcc cggcctcttc cagaagccga tagttttgca
     4381 tgtttctcgc gccgatttgc aaaatatcag cgtattgtgc taccagttcc acatctgagg
     4441 gcgtgatgac ctcggtcacg acgggcaaac cggtttcctg ccgggccagt gctaaaatct
     4501 tgaggccttc ctcttttaat ccttggaaac tatacggact ggttcgaggt ttgaaagccc
     4561 ctccgcgcaa gcccgtcgct ccagcggctt taaccgcccg ggctgtggat agtgtctgct
     4621 cttctgtttc caccgaacac ggtccagcga tgacaccgat atgggaattg ccgcaaacga
     4681 atgggccgac cctgaccacc gtgtgttccg gattgacctc gagacttgcc actttgtaag
     4741 gagctagaat gggaatcacc tcagccaccc cgggacaact ctccaaagag gcacgatgct
     4801 cgtcccgctt atcgccgatg gccgcaatca ctgttcgctc cgtcccgtaa atcacatggg
     4861 cacgcagtcc aaaggcttga acgcggtcaa ccacgtattc gatctgcgat tgcgatgcac
     4921 ctcgtttcat gacgacaatc atgggcggca gctcctttct cagacgttag ccacgatctt
     4981 gggaaaaaca aaaaagccct ggcgaccttt atgggttgcc agggcttttt actgtctgcg
     5041 tctatcgaat ctcgctgcta cagacctcct ccggccccgc aaccctgcgg gctaaaataa
     5101 aaccagtagt aggtcttgca gcaggtactc atgtccgatc aactcactcg gatgttaacg
     5161 actctactaa ctataagtcg cctcaagcaa aaaatcaatt ccttcggcaa acaaagtgat
     5221 tatcggccaa gcctcgctca aacctttagt gacaatgcta ggccgattcg atttctacgg
     5281 ctacccccac gtctgggggt acacctcaaa gatcaggcta ctctaaaagc ccgacccgtc
     5341 cattggattg cgccaatttc ttggcttccg tgtcactcat cttcgacgct gtcttttccg
     5401 tgagcatata atttcactgt cacaattgtg agacgaatcc ctagcttacg tggaaaggag
     5461 aaattcatcg tgtccgattg ggtcgaagta ggtcagccgg cgccggactt tacactcccg
     5521 tcagatagtg gagaacacgt ccgattaagc cagtttcgcg ggtcaccggt cgtcttgtac
     5581 ttttatccca aggacgatac cccaggatgt acgcgcgagg cctgtgcctt ccgcgatttg
     5641 aagcaagaac tggtgaaact cggtgcggtg attctcggtg ttagccccga tggtgtggaa
     5701 agtcacaaga aatttcgcga gaagttcaat ctgaactttc cccttcttgc tgacgtcgat
     5761 catagcgtgg cggaagcata cggtgcctgg cgagaaaaaa cccgcttcgg aaagaaatcc
     5821 atgggcatcc agcgttccac cttcttgatc gatgccaatg gcgttgtcag aaaagtatgg
     5881 aagaacgttc aggttgacgg tcatgatgac caggtacttg aggccctcaa ggcattgacg
     5941 caagagtgat ttctaggcac ggctataacg gcttggtggg tgatcgcgat gtaccaaact
     6001 ccgccgatcc ctcgcaaaat ctggaacact gatttatgaa gcgattttgg ttcatcctgg
     6061 tcaactgtgg cgcgattgtg ctaagcgctg ggttgggttg tcgaaatgaa cgagaagcga
     6121 acgtgaccgt tttggaacct caaaagacga tcatcgttcg tgccgaaaat accgcccagc
     6181 cacctttgcc cgcccagcca tccggcgagc aaccgaacgc ccccaccatc ataccgcaac
     6241 cgggcgatag tgtgggcaac gcaaaaccgg aaataacagg cgcaacggcg aacaatcgtc
     6301 agatcgtgct ggggggacga ttccgggcca cagtgccggc tcattgggtg gccaaaccgc
     6361 cggcgatttc gttgatcgag cacgagtttg ccgtgccctc gacgattgaa ggcgagccag
     6421 atgggcgggt gaccttctcg tcagcacgtg gaggtgtcca agccaacata gaacgttggg
     6481 caaaccagtt ccgctttgcc gccggtcagg acctacgtca ggcggtcaaa catgaaaaga
     6541 cttccattct cggtgagaat ctccatttgg tcgatatctc gggaacctac ttggagacat
     6601 tcggaccgag gatggaaaag gccgtcgagc gagaaggcta tcggatgctt ggggccatca
     6661 ttgaatgcca ggacggtttt ctctatttca tcaagttcta cggtccccag gcaaccgtca
     6721 aagaaaacga gttggccttc cgggcgatga tcgaaagttt gcaggccgtt ggcccacagt
     6781 aagcagtgct tgtcggcgat ttttgtgcca tccctggagc cgtttttccc ggtaaattgg
     6841 gcacccgatc ttgacgggaa cgagacaatg gcatatggtg ataacagaac ggcccatgtg
     6901 attcgggccg gttattgggg tatggtgtaa cggtagcacg actgactctg gatcagttag
     6961 tctaggttcg aatcctagta ccccagctag gcactattca aaacgaatgc cagcggggcg
     7021 cggcgatttc tgctctcttg gtcaggaaat tagtgggccg ttatcttagg ccctctcgac
     7081 ttgtgacttc tcgataagaa accaagccat ctgaggcaat gggcggccct agcagcaggt
     7141 tgctttctgc acgacgacca tgaggcgggc tgtacttggc acgagtcgaa ttccagtttc
     7201 tccacgggtt tcaccgcgga gttccgcaga aaccaaaaag ctcaaggtct tgtacttcgc
     7261 caatgagttt agaaagccaa gtcgttttat caagcgatcg gggccggcgc acatggtcgt
     7321 agagaaccta tccccaaaag tccccctctc cctccgggag agggttgggg tgagggcaag
     7381 cgaacccgga atcgattgag aaaaccggca gcgattcgag tcaccctcac ccccgccccc
     7441 atctcccgcg gaacgggcca ggcgagatta tgggataggc tcagaagagt gattgtcccg
     7501 cggtgaggca agtgattggc tagcagagtt gagcgtgtag gaccagcggc aaccgtgatc
     7561 agaaactggg attgagaaca aattccgatt cccttgtcaa gatggcttct gcccaggatc
     7621 agcggagctg cttatcaaac ccaaaatctt ggaattgttc tcttgtttcg tgcgtcctga
     7681 ttctattagg atgaacgtgt tggggatttg ggctcgactt tggtgacgta tgtttaattc
     7741 acagtccctc accacgggga gagcaggcga tgattcgtta catctgcgat ctgtgtcggc
     7801 gagagatcga ttcaacccgc gaagtgtgtt accacgtgga gattcagatt tcggcggcct
     7861 ttgagccagt cacggaagac gagccggacg atcgcgatca cttggaagag attcatgatc
     7921 ttctcgaaag cctcaaccaa ctcggtgacg aggcctatga atcggcccat gagacgttac
     7981 ggttcgattt gtgtcc
//
LOCUS       DAOSUT010000178         7969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4627_length_7969_cov_10.055977, whole genome shotgun sequence.
ACCESSION   DAOSUT010000178 DAOSUT010000000
VERSION     DAOSUT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4627_length_7969_cov_10.055977"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="PLD05_13455"
     tRNA            complement(3..74)
                     /locus_tag="PLD05_13455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Gly,seq:ccc)
     gene            complement(246..869)
                     /locus_tag="PLD05_13460"
     CDS             complement(246..869)
                     /locus_tag="PLD05_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOP78495.1"
                     /translation="MKNDIEFIYFDLGRVIINFDNDRMCQQMAQVSGVPCERVREILF
                     ATGLQRQFELGEITPDEFFDKYCHLLGRSVDRNALTLAANDIFWLNLPMLPVVAQLQA
                     AGWPLGILSNTCSPHFEWCRSHYAILQKGFRCYALSCDLHALKPDHTIYEKAARLAGV
                     APEKIFFTDDIPAHTEAARAFGYDAVPFQSPRQLVKELRDRGIRFNY"
     gene            1124..3133
                     /locus_tag="PLD05_13465"
     CDS             1124..3133
                     /locus_tag="PLD05_13465"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOP78496.1"
                     /translation="MMLRTRVLWIVFGLLCWLATPCLAWDDVKQIPLSTLDLSHMSQG
                     WGRPVPKGNVVKQALRIGGKDYQEGVGTHAVSVLYLALDGQVLRFTAEVGIDESAGRR
                     GSVRFQIYADKRLVFDSDVMHGGDPAKPVDVNLEGVKELLLFVDGTEDGIDFDHADWA
                     NAVFYYRGQPPHTVAAPEEPREILTPKPGPAPQLNHAGRYGCRPGRPFIYRIPCTGER
                     PIKFSAMNLPSTLHLDPATGIITGHAPRQRGEYEVQLIAENQHGKDVGKWTIVVGDTL
                     ALTPPMGWNSWYIHYHRVSDADMRAAADAMIDSGMADFGYCYVNIDDCWMVKPGSDDP
                     KLGGPPRDEAGAVRPNGKFPNMKALTDYIHSKGLKAGIYISPGPLTCAGYVGSYQHEE
                     IDAKKFAEWGFDFLKYDWCSYGRVVQAKTREDFMKPYRLMGDILKRLDRDIVYNLCQY
                     GMDSVWEWGAEVGGNCWRTTGDLGLAGGSLNRGIYQVGLKNAKLWEYARPGHWNDPDY
                     LLIGWVGDARVTGEGRPTPLTPNEQYSHMSMWCLMAAPLIFSGDMTRLDDFTLNILCN
                     REVIAVDQDILGQQARIVRQTATELVLAKPLVDGSVAVGLFNLGEFPQKMTITWQELN
                     RQGPQRVRDLWRQKDLGISQDGYTSTVGRHGVTLIRVTTAKESQN"
     gene            complement(3196..5070)
                     /locus_tag="PLD05_13470"
     CDS             complement(3196..5070)
                     /locus_tag="PLD05_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOP78497.1"
                     /translation="MLRSVITSLVFLVIYLNLAIGYGDGLSTTLVANGRQRAIPRPFE
                     HHPGNVFLEGEPIRISLPVAGSWSLYDAEDQLLKEIRTDGHEIDLGTLSVGFYRLRNS
                     RVSDWVSLAVIARLQAPTPETSPICIDVAMSWFYPENKMEDVASLCALAGVNWVRDRL
                     SWGEVEPQRGEFVAKTKYDAAATAQAAAGLKVLQVFHSSPRWANPNGKRFPLDLRDAY
                     RFLRHISERWKGKVLAYEPWNEADIEVFGGHTGAEMASLQKAAYWGIKAGDPDAIVCW
                     NVFASSNPAQLADVDANEAWPYFDTFNFHHYRPLEQYPRFYADFRAISAGRPLWVTEC
                     AMPLRWTGDPKKQELSDQDLYEQARRLIKVFAGSLHEGTAATFYFLLPHYVEGQTQFG
                     IIRRDLTPRPAYVSLAAVGRLLADARPLGQLGTDKLLGYAFSAKPDGQSRLVLVAWSP
                     EPLTFSLPEQPLAVYDYLGREKAANQEIALTSSPVFLVFPETAVPAFSLREPPKPAPA
                     RPGTPCPVVLQATWPEDQVDLKQSAYRVPKDAKEITIPIFGYNFADKPLRGRFTVKVP
                     DGWTADFLNEAPLAPQERLQWELRLTPGQFGADQPVVIRIEGDFGDSGRSVLSFRIVQ
                     "
     gene            complement(5145..7811)
                     /locus_tag="PLD05_13475"
     CDS             complement(5145..7811)
                     /locus_tag="PLD05_13475"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOP78498.1"
                     /translation="MVAQVPAHTMMAKGRLFWRKPVAWLAVLGVLIDVMIVASALAET
                     QGHGILLSQDGVAQCAIVVPQGSSPKELELASELAHWLKEMTGATFDVSRGLRDQAII
                     LGTWDEWRTLLSADMEPPKGPEGYRIRSDRKNLWIIARSELGLQHAIYDFLECLGCRW
                     YFPDPVWTVIPRVPTLVIQLDRTAVPAFGYRRIWYGWGPRTDKLARDYQDWLKHNRQL
                     GSFAVSCGHSYDSHIPPSQFAEHPEWFAYVNGRRQPSQLCTSHPEVQKRVIEHVLEVF
                     RKNPAANMVSVEPNDGDGYCECEACLKLGTPSDRVFHLANVVAEAVRRKFPDKWVGLY
                     AYAGHSEPPHFALKPGVYVQVTTGFRYTKLTFEEQVEAFRKLGAAVGVYDYFSVYPWD
                     WDMPGAAKASRVFELAAAIRHYHELGLTTYDAESSCNWGPNGLGYWMAAHLMWDPNLD
                     PETLFQDFCDRAFGPASQHVARIYRRWADGERLSPRQLKLALLDLQQAYASTDDPTIR
                     QRLDRIAMYLHWLRLWSDYDRSARWNQWGRLAVAPSETILRHAEQFVRYTRRIMDTGL
                     VHSYPALHSEWFRHRLAALAKIPGFDWEQTKAWMRDDRLPTAEEISQDFHEDLRGLQE
                     VQAVELKKMRFEHNLVPLAARQPALVAEWQKVAPSNVAVESGVFVFRADKTEALPLEY
                     RPYDTGHTIDCNWKLCRWPDISHPLVNGHVKAAKGEKGPVEINVPGPGLFAFDPGTAY
                     WRAAEIGFDHRPLSLWAGRPKDDTKGFPPLRLWRPRLDQPLYLFVPKGTSDLVIGIVE
                     GGDPYTVIQVKTSKGTVLCQERLLAGDQLCVSVNPQARDRDDPTDEDKAIRGGKVLSV
                     GFDSLRCVIEIYNVPPYLARHPSELLVPVDAM"
BASE COUNT         1820 a         2163 c         2242 g         1744 t
ORIGIN      
        1 agagcgggag acgggattcg aacccgcgac ccccagcttg ggaagctagc gctctacccc
       61 tgagctactc ccgcaatcct cctggctttt gccaggctaa ctattaccag cgtacggttt
      121 ggtcgcctgc gtgtcaatgc ccaggttcgg gacctttcac cagaggccgg ggatgaccac
      181 ccacgggtcg gcccggtgga gggctcccgg caagataacc tgacatcggt atcagctcgg
      241 tccgatcagt agttaaaacg aatcccccga tcgcggagct ctttgaccaa ctggcgaggc
      301 gattgaaacg gcaccgcatc atacccaaag gcacgtgccg cttccgtatg ggcggggatg
      361 tcgtccgtaa aaaagatctt ttcaggtgca acacccgcca gacgggcggc tttttcataa
      421 attgtgtgat ccggcttaag ggcgtgaaga tcacaactca gcgcgtaaca gcgaaaccct
      481 ttttgaagga tggcgtagtg actgcggcac cattcaaagt ggggcgaaca ggtgttggag
      541 agaatcccca gtggccagcc tgcggcctgg agctgggcca caacaggcaa catcggaaga
      601 ttgagccaga aaatatcatt ggcagccagc gtcaacgcat ttcgatcgac acttcgcccc
      661 agcaagtggc aatatttgtc aaagaactca tcaggtgtaa tttcgccaag ttcaaattgc
      721 cgctgtaatc ccgtggcgaa gaggatttct cgaacgcgct cgcatggcac accagaaacc
      781 tgcgccatct gctggcacat ccggtcattg tcaaaattga tgatgacacg gcccaaatcg
      841 aagtagatga actcgatgtc gtttttcatc acgttgcgct gtcactaata acaattgagt
      901 tgcaacggta ccaccctttt gcgaattgcg cgaagcccac agtctcctct actgtggtct
      961 tgccaaggac gccacccttg cggattctag ccctggcgat tacaacgatt acggacaacc
     1021 gatgtagtta gttcggcgat tccgtgaacc ttgcaaaaac gcgcttcgtt gtcaatagcg
     1081 gcgaataaac cgttgtcgaa gcacccgcgt ctttggagga acgatgatgt tgcgaacacg
     1141 agttctctgg atcgtttttg gtttattatg ttggcttgcc accccgtgtt tggcgtggga
     1201 tgacgttaag caaatcccgc tttcgacgct tgatctctcg cacatgtccc aaggatgggg
     1261 acggccggtg ccaaagggaa atgtcgtgaa gcaagccctc cgcatcgggg gcaaagatta
     1321 ccaggagggt gtcggtactc atgccgtgtc ggtactttat ctggcgctcg atggccaggt
     1381 cctacggttc actgcggaag ttgggatcga cgagtcggca ggacgcaggg gaagcgtcag
     1441 atttcagatt tacgctgaca agcgactggt tttcgacagt gatgtcatgc atggtggtga
     1501 tcccgcgaag ccggtcgatg tcaatctgga aggtgtcaaa gagcttctcc tttttgtgga
     1561 tggaacggag gacggcattg attttgatca tgcggattgg gctaatgctg tgttttacta
     1621 tcggggccaa cctcctcaca cagttgcggc gcccgaggag ccacgcgaga tattgacgcc
     1681 caagccgggc ccagcgcccc agctcaacca cgcggggaga tacggatgcc gtcccggtcg
     1741 tccgttcatt tatcggattc cctgtacggg agagcggccg atcaaatttt ccgcgatgaa
     1801 tttgccgtcc acactgcact tggatccagc aaccggcatt atcactgggc atgcacctcg
     1861 gcagcgcggt gagtatgaag tgcaactcat tgcggaaaac caacacggca aggacgtggg
     1921 gaagtggacg attgtggtcg gcgacacctt agctctcacc ccgcctatgg gatggaatag
     1981 ctggtacatt cattatcatc gggtgagtga tgccgacatg cgggcagcgg cggatgccat
     2041 gattgactcg ggcatggccg acttcggtta ttgctacgtc aacatcgacg actgctggat
     2101 ggttaagccg ggttcggatg atcccaaact gggtgggcct ccccgggatg aggctggggc
     2161 agtccgaccc aacggaaagt ttcccaatat gaaggcccta acggattaca ttcactccaa
     2221 ggggttgaaa gcaggcatct acatttcgcc gggaccgctc acttgtgcag gatatgtcgg
     2281 aagttatcaa cacgaagaaa ttgacgccaa gaagttcgcc gaatggggct tcgatttcct
     2341 caagtatgat tggtgttcct atggacgtgt tgtccaggcc aagacccgag aggactttat
     2401 gaaaccgtac cggttgatgg gcgatatcct caaaaggctg gaccgagata ttgtgtacaa
     2461 cttgtgccag tatggaatgg actctgtgtg ggagtggggc gcggaggtcg gtggtaactg
     2521 ctggcggacc acgggcgatt tgggcctggc gggcggctcg ctcaatcgtg gcatctacca
     2581 ggtggggctc aagaacgcca agctttggga gtatgcacgc cccggacact ggaacgaccc
     2641 cgattatttg ctcatcggat gggttggtga cgccagggtc accggtgagg gacgccccac
     2701 accgctgacg cccaacgaac aatactcgca catgtcaatg tggtgcctca tggcagctcc
     2761 cttgattttc tcgggcgaca tgacgaggct cgatgatttc acgctgaata tcctgtgcaa
     2821 tcgcgaggtc atcgccgtcg accaagatat ccttggccaa caggcgcgaa ttgtgcggca
     2881 gacggccacc gaattggtcc ttgccaaacc actggtggat ggttccgtag ctgtggggct
     2941 attcaacctc ggtgaatttc ctcagaagat gaccatcact tggcaggaac ttaaccgaca
     3001 gggcccacaa cgggttcgtg atctctggag acagaaggac cttggaatct cccaggacgg
     3061 ctataccagc acagttggcc gccacggcgt tacgttgatt cgtgtgacaa ccgcgaaaga
     3121 aagtcagaat taaccccgat gtgttgaacg agccggataa caagcgagcg tgcgccgtct
     3181 ggtccacacc tcgccctact gtacgatccg gaaggaaagc acagaccggc cggagtctcc
     3241 aaaatcgccc tcaattcgga tgactaccgg ttgatcggcg ccaaactggc cgggtgtgag
     3301 gcgaagttcc cattgaagcc gctcctgcgg cgctaacggt gcttcattta ggaagtcggc
     3361 tgtccagcca tcaggtacct tgacggtaaa acggccacgg agcggtttgt cggcaaaatt
     3421 atacccgaat atggggatgg tgatttcttt ggcgtctttg ggcactcggt aggctgactg
     3481 cttgagatct acttggtctt ccggccaagt ggcttggaga acgaccgggc aaggggtacc
     3541 tggtcgggct ggcgcaggct tggggggctc cctgagtgaa aacgccggga ccgctgtctc
     3601 gggaaaaacg aggaaaaccg gtgacgatgt caaggcgatt tcctggtttg ccgctttttc
     3661 ccgcccgaga tagtcataga cggcgagggg ttgctccggc agtgagaacg tgaggggttc
     3721 cggtgaccag gcaacaagga caagtcgaga ttgcccgtcg ggcttggcgc taaaggcata
     3781 gcccaggagc ttgtccgtgc ccagttgccc aaggggccgg gcatccgcaa ggaggcgtcc
     3841 aacagcggcc aaagaaacgt aggcgggccg gggtgtcaga tcacggcgaa tgatgccgaa
     3901 ctgtgtctgg ccctcaacat aatgcggcag caggaagtaa aaagttgccg ccgtgccttc
     3961 atgaagggac ccggcaaaaa ccttaatcaa gcgccgggcc tgttcgtaca agtcctgatc
     4021 gctgagctcc tgtttcttgg gatcgccggt ccagcggagg ggcatggcac actcggtaac
     4081 ccagagaggc cgtcccgccg aaatggcccg gaaatcggca tagaaccgcg gatattgttc
     4141 caggggtcgg taatggtgaa agttaaatgt gtcgaaataa ggccatgctt cgttggcatc
     4201 gacatcggca agttgtgcag gattgctgga cgcaaaaacg ttccagcaga caatggcgtc
     4261 ggggtcaccc gccttgattc cccagtaagc cgctttttgg agggaggcca tttcagcccc
     4321 agtgtgacca ccgaatactt caatgtcggc ctcattccaa ggctcatatg ccaagacctt
     4381 ccctttccaa cgttcggaaa tgtgtcgcag aaagcggtag gcatcacgca agtctagggg
     4441 aaaccgtttg ccgtttggat tggcccagcg tggcgagctg tgaaagacct gcaagacttt
     4501 caggcccgcc gctgcctgag cggttgctgc cgcgtcgtat ttggttttgg ccacgaattc
     4561 ccctcgttgt ggctcaactt ctccccacga cagacgatct cgaacccagt taacccccgc
     4621 tagcgcacat aaacttgcta cgtcctccat tttgttttct ggataaaacc aactcatcgc
     4681 gacatcaatg caaatgggag aagtttcggg cgtgggcgct tgcaggcggg caattacagc
     4741 caacgaaacc cagtcgctca cccgggagtt tcgaagtcta tagaatccca cagagagagt
     4801 tcctaaatcg atctcgtgtc cgtccgtgcg gatttctttg agaagctgat cttcggcgtc
     4861 gtacaacgac caggaacccg cgacgggaag agaaatgcga atcggctcac cctctagaaa
     4921 aacgttgcct gggtggtgct caaatggccg cgggatagca cgttgacggc cgttcgcgac
     4981 aagcgtggtt gaaagtccgt ctccgtatcc gatagcaaga tttaaataaa ttactagaaa
     5041 cacaagcgat gttattacgc tgcggagcat gggtcacctc ataggttggc gagagactcg
     5101 taaacctttg ttagctgccc ttctcaacca acataacaat agcttcacat agcatcgacc
     5161 ggtaccaaga gctccgaagg atggcgcgcc aggtacgggg gaacgttgta aatttcgatg
     5221 acgcagcgga gcgaatcaaa cccaaccgag agaaccttcc ctcctcggat ggccttgtcc
     5281 tcatcggtcg ggtcgtctct atcccgcgct tgtggattga cactcacgca aagctggtca
     5341 ccagctaaga gccgctcctg acaaaggaca gttcctttgc tggtcttgac ttggatgacc
     5401 gtgtatgggt ctccgccttc cacgatgccg attaccagat cggacgttcc tttggggacg
     5461 aacaagtaca acggctgatc gagacgcggc cgccacagcc gcaaaggcgg aaaacctttt
     5521 gtgtcgtctt tgggacgccc ggcccagaga gatagcggtc tgtgatcaaa gccaatctcc
     5581 gcagcacgcc agtaagctgt tcccggatca aaggcgaaca agcccggtcc gggcacgttt
     5641 atctcgacag gtcctttttc acccttggct gccttgacgt gaccattgac gagggggtga
     5701 ctaatgtctg gccagcgaca gagcttccaa ttgcagtcaa tggtgtgtcc ggtgtcatag
     5761 gggcgatatt cgagaggtaa tgcctctgtt ttatctgcac gaaagacgaa cactcctgat
     5821 tccacagcaa cgtttgacgg ggcaaccttc tgccattctg ccaccaatgc tggttgacgt
     5881 gctgccaatg ggacaaggtt gtgctcgaat ctcatctttt tgagctcaac cgcctgaact
     5941 tcctgaagtc cccgcaaatc ttcatggaaa tcctgggaga tttcttcggc agtcggcaac
     6001 cggtcatcgc gcatccaggc cttcgtttgc tcccaatcga accccggtat tttggccagg
     6061 gcggcaagcc ggtgtcgaaa ccattcggag tgcaacgcgg gataactgtg gactaagcca
     6121 gtgtccatga tcctacgggt atatcgaaca aattgctcgg catgacgaag aattgtttca
     6181 gaaggtgcca cggctagccg gccccattga ttccaacgtg cagagcggtc gtagtcactc
     6241 caaagtctta accagtgaag gtacatcgct atccgatcaa ggcgttggcg aatcgttggg
     6301 tcgtcggttg atgcatacgc ctgttggagg tccaagaggg cgagtttaag ctgccgcggc
     6361 gaaagccgtt caccgtcggc ccaccggcga tagattcgtg ctacgtgttg gctagctggt
     6421 ccaaaagcac ggtcgcaaaa atcttggaag agcgtttcgg gatccaaatt gggatcccac
     6481 atcaagtgag ccgccatcca atagcccaag ccattgggac cccagttgca agacgactcc
     6541 gcatcatatg ttgtcaatcc caactcgtga taatggcgta tggctgcggc caactcaaag
     6601 acgcggctcg cctttgccgc accaggcatg tcccaatccc acggataaac actgaaatag
     6661 tcataaacac ccaccgcagc ccctaatttg cggaaagctt caacctgctc ttcgaatgtg
     6721 agtttcgtgt atcgaaatcc tgttgtaacc tggacataaa cgcccggttt tagggcaaaa
     6781 tgcggcggct cactgtggcc ggcataggca taaaggccca cccatttgtc cgggaatttc
     6841 cgacgaacag cctcggccac gacgttggcg agatgaaaga cgcgatcact cggcgttccc
     6901 aacttaaggc acgcttcgca ctcacagtat ccgtccccgt cgttaggttc cacggatacc
     6961 atgtttgcgg ccgggttctt gcgaaagacc tctaacacgt gctcgatcac gcgtttttgc
     7021 acctcgggat gagatgtgca aagctgtgaa ggctgccgcc gaccgttgac atatgcgaac
     7081 cactcaggat gttccgcaaa ctgactcggg ggaatatggc tatcgtacga gtgaccgcat
     7141 gacacggcaa aggacccgag ttggcgatta tgtttcaacc aatcttgata gtcgcgggcg
     7201 agtttgtctg ttcttggccc ccagccgtac caaattcggc ggtaaccaaa agcagggact
     7261 gctgtgcgat caagttggat caccaatgtg ggcacgcgtg ggataactgt ccaaacgggg
     7321 tccggaaaat accaccgaca tcccagacat tcaagaaagt cataaatcgc gtgctgaaga
     7381 cccagttcac ttcgagcgat gatccaaagg tttttccgat cgctgcggat tcggtatccc
     7441 tctggccctt tgggtggctc catgtcggca cttaacaagg tacgccactc gtcccaggtt
     7501 cccaagataa ttgcctggtc gcgcaatcct cgtgaaacat cgaaggttgc tccggtcatt
     7561 tcttttagcc agtgggccag ctcagaagcg agttcgagct ccttggggga cgacccctgc
     7621 ggtacgacaa tcgcgcattg agcaacaccg tcttggctta gcaagattcc atgcccctgc
     7681 gtctctgcga gggcgctcgc gacaatcatc acatcgatca aaacaccaag caccgcgagc
     7741 cacgcgaccg gttttctcca aaatagacgc cccttagcca tcatggtatg agcaggcact
     7801 tgagcaacca tacttatgct ccgacgaatg gttggtccta gaagtcatct gcttaatgcc
     7861 tattccataa tctcccctcg cccgtttggc gggagagggg tcgggggtgg ggggcttgaa
     7921 tcactggcgg ttttctcaat cgattccgac gttcgcttgc cctcacccc
//
LOCUS       DAOSUT010000179         7905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4664_length_7905_cov_9.311975, whole genome shotgun sequence.
ACCESSION   DAOSUT010000179 DAOSUT010000000
VERSION     DAOSUT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_7905_cov_9.311975"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLD05_13480"
     CDS             complement(<1..1031)
                     /locus_tag="PLD05_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78499.1"
                     /translation="MSYRWLLLASPVAGFLLTVTGTGQVLGEEPSRVLPEGPGLAAQC
                     GDTEVLAKHPEVVFMEDFEVGQFSDLKGRWTQISHPEIMTLSDLVPPDSHGKRSLLMT
                     HTGGKGTGGHLYRRLNQGYEKLFIRFYVRFAPDCGEIHHFFHVGGYNPPTPWPQGGAG
                     ERPRGNERFTVGIEPFGRDWRWDYYTYWMEMRGSPPRGQTWGNCFINDPKLRVKKGEW
                     QCLEVMIKLNSPVTESNGELALWVDGKLVSHLGPGFPKGKWTYDRFLPGQGGRAIRWN
                     DEKGGPETFSVPEGGEPFPGFRWRNDEDLKINFVWVLLYITKTPPGQVSQVWFDDIVV
                     AKSYIGPNK"
     gene            complement(1129..2394)
                     /locus_tag="PLD05_13485"
     CDS             complement(1129..2394)
                     /locus_tag="PLD05_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78500.1"
                     /translation="MKRFFTSLVLLCALVSQKALGESAGGLVLPPLPPSILRTAPPLP
                     PPAGPAIRVRTVQELYRAAEVVPPGGTILLANGHYVLPRRLDIHTDRVTLRGESGRRE
                     LVVLDGGPHQLGELLAIRQCSGVTIADLTVQNVRWNGIKLDTDSGVHQVTIHNCIIHN
                     VWQRGVKGVRIPKTIPRPRDCRIQWCLFYNDRAKSYADDPADTPENFRGNYIGGIDVM
                     FPQNWVISDNVFYGIQGRTRSGRGAIFLWHEAENCIVERNVIVDCDVGIALGNSFKPD
                     DVATHAIGVIARNNFVVRCPESGIVADFTRDCLIAHNTIFDPDNRLDRLIRLVHDNPG
                     LVVANNLLVGPPVRNESPSEIRFLTNVVLAGDPGAFADPMRGELHLTKSLESVVNGGS
                     VLPQVSEDFDGQARDDHPDVGADEYQVPP"
     gene            complement(2723..3382)
                     /gene="trmB"
                     /locus_tag="PLD05_13490"
     CDS             complement(2723..3382)
                     /gene="trmB"
                     /locus_tag="PLD05_13490"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOP78501.1"
                     /translation="MGRRALRKIDPNLDLSFHYRELPNIPKPFQAEVIFGRSAPLEVE
                     VGSGKGLFLRRAAAGTPDVDFVGLEIAQKYARFSAAGLAKLGVRNAIVIHGDAQLFFA
                     EWLLPESVQAVHVYFPDPWWKKRHKKRRVLNETFLKNVQRALRPEGRLHFWTDVAEYF
                     QTTIELLKNTTSLLGPFPVPERPAENDMDFRTHFERRVRLQGGTIYRAEFVKPSSSVL
                     Q"
     gene            complement(3601..4146)
                     /locus_tag="PLD05_13495"
     CDS             complement(3601..4146)
                     /locus_tag="PLD05_13495"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOP78502.1"
                     /translation="MPKKPLILDPAEIDTSRIIADIEEIRRYNPQRYEMEQLTAICYE
                     DLERAICAGYKDLGPDEFWVRGHFPGMPIMPGVIMCEAAAQLASYYTIKHKLMKAPMV
                     GFGGLQDVRFRGVVRPGDRFLIVARLLKVRSTMLTCEFQCFVRENLVCEGVILGIPLF
                     PETIEGSRDNGAETASADAGA"
     gene            complement(4682..6004)
                     /locus_tag="PLD05_13500"
     CDS             complement(4682..6004)
                     /locus_tag="PLD05_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOP78503.1"
                     /translation="MIASSSAIGAVRRPFKLWTTSRADSLLLALVLLTLLAGIWLGHP
                     FWHDDSLGVPITLDRIAWLTVLLGTVFITVRRGVTFTRIARGDLFLAVLVAYLGALAV
                     MQFSSEHTGAAARWVFYYFMPASLYFALRQIHLSSSAERGILLVLGCVGVCLSLTGLF
                     EVFGLRSLIYPAYIMDRSVSGEFLGRARGPLLNPIINGFIMIVAWAAWVFLGRSAPRR
                     WWPGLVLVHGIMAVGVISTLTRSVWLAAGLAGLFLGYHLVPGGYRRAWLATAGFGLIV
                     MALVVKGFFWEMKRDQGLSPAEAARSAQLRPILALVAWEMAKDHPFFGVGLGQYDRAK
                     TKYLANPLASYPVHWAKPYTQHNVFLSILVETGAIGLMVFLFLLAAWTRDMCSAVSQG
                     NLVFPLMAIVAIVAYVTNGLFHDVSIIPQMHTVLFVTLGLAESSADSR"
     gene            complement(6001..7707)
                     /locus_tag="PLD05_13505"
     CDS             complement(6001..7707)
                     /locus_tag="PLD05_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78504.1"
                     /translation="MPTTSQTSFSGKIWLAKRLIIERPVRTSLVASFLLFATIATYGV
                     LKGPEWEATQPLVIRAEALEGSKIPGRFESDQQRRHVLDTLVAVFQSQDVLEATLREV
                     GPARPVRRHSFPDRRDIERLRRSVQIVPPSDTDFGTTELVYLKVRDARPERAGRLASV
                     LVEQADRRLREVQAAKAEATVQELVFTEKSANDVLQNLKEELAALEAQLGLDSLTLRA
                     YEEKPDESLLRSAIEELDDQLDELEAQIVLQEELLRLLRGTLATPEALASIPPAMLEK
                     YPTLKRFQEALTDAEVKLIELRGQYADEHPAVIAAQLALQDLTNRISEEVPTVIQTIE
                     NEQAVAANQKQFLEQKRQTEEARVQHILAVLPKYRQLMAAIRAQSETVRDAQHRLAIA
                     RAGAASARSARLITAVEPPRTGNRPVGPGTLTIVAGGFVCGLFLALCVFLWLTPVAPS
                     ETKPIVTSHAPTHEEDGDQPVQAEMVLPTAKKETRIPSEADVTSHGPLFDSPGAEAQP
                     ALASTAIQPTTPDGLWKDRASQADTPTNWPAIPVAEDWAESPLHQALAGMAQSRDPGG
                     KA"
BASE COUNT         1801 a         2217 c         2229 g         1658 t
ORIGIN      
        1 tatttattcg gcccgatgta gctcttggcc acaacgatat cgtcgaacca gacttggctt
       61 acttggcctg gcggggtttt ggtgatatac aagaggaccc agacgaagtt gattttcaga
      121 tcttcgtcat ttcgccagcg aaagccagga aagggttctc ccccctcggg tacggaaaag
      181 gtttctggac cacctttttc gtcattccag cgaattgccc gaccaccctg accggggagg
      241 aagcgatcgt aagtccattt ccctttaggg aacccgggac cgagatgact aaccagtttt
      301 ccatccaccc agagggccaa ctcgccgtta ctctccgtga ccggggaatt gagtttgatc
      361 atgacttcca ggcattgcca ttcgcctttt ttgacccgaa gtttgggatc attgatgaaa
      421 cagtttcccc aggtttgccc gcgcggtgga ctcccgcgca tttccatcca gtaggtgtaa
      481 taatcccacc gccagtcgcg tccgaacggt tcgataccga cagtaaaccg ctcgtttccg
      541 cgaggccgct cgccagctcc gccttgtggc cacggggtcg gagggttgta tccgcccacg
      601 tggaaaaagt gatgaatctc gccacagtca ggggcgaatc gcacgtagaa cctaataaac
      661 aacttctcgt agccttgatt cagtcggcgg tagagatgac ctccggtccc tttaccaccc
      721 gtgtgcgtca tcaacagcga ccgcttaccg tggctgtcgg gcgggacgag gtcggagagt
      781 gtcataatct ccggatgcga aatttgcgtc catcgccctt ttagatcgct gaattgccct
      841 acctcaaaat cttccatgaa gacgacttcc gggtgcttgg caaggacctc cgtgtctccg
      901 cattgggcgg ccaaaccggg accctcgggc aatacccggg aaggttcctc ccctaagacc
      961 tgccccgtgc cagtaaccgt tagcaggaat ccagcgaccg gagatgccaa gagaagccag
     1021 cgataactca tcgttacgtc ctcccactaa gattgccgaa aacattcaaa gtcggaaacg
     1081 gaaatatggc tttccttttt cagaaagcaa acacgacggc ccgttgggtt atggaggcac
     1141 ttggtactcg tcagcaccta cgtcgggatg gtcgtctcgg gcttgcccat cgaagtcttc
     1201 ggacacttgc ggtaagacgc tcccaccatt tacaaccgac tcaagactct tggtgaggtg
     1261 gagctctcct cgcattgggt cggcaaacgc accggggtcg cccgccaaga caacgtttgt
     1321 caaaaaacgg atttcgctag gcgattcgtt tctcacggga ggtcccacga gtaagttgtt
     1381 cgcaaccacc aaaccagggt tgtcgtgaac caaacggatc agacgatcaa gacggttatc
     1441 cgggtcaaaa atggtgttat gggcaatcag gcagtcccga gtaaagtcgg ccacgatgcc
     1501 tgattcggga caccggacaa caaaattgtt cctcgcaata acgccaatgg cgtgggtagc
     1561 aacgtcatcc ggcttaaagg aattaccgag agcgatgcca acatcacaat ccacgattac
     1621 gttccgctcg acaatgcagt tttcggcctc atgccagaga aagatggccc cgcgccctga
     1681 ccgggttcgc ccttggatgc cgtagaacac gttgtcgcta atgacccagt tttgagggaa
     1741 catgacgtca atgccaccga tgtaattgcc ccgaaagttt tccggagtgt cagctgggtc
     1801 atctgcgtac gacttggccc ggtcattata gaagagacac cactggatac ggcaatccct
     1861 ggggcgtggg atcgttttgg gaattcgtac tcccttcaca ccccgttgcc agacgttgtg
     1921 gataatgcag ttgtggatgg ttacctggtg aacgcccgag tccgtatcaa gcttgatgcc
     1981 gttccagcgc acgttttgca cggtcaaatc ggcgattgtc acaccgctac actgacggat
     2041 ggccagcaac tcgccgagtt gatgcggtcc gccgtcgagc accacgagct cccgtcggcc
     2101 agattctccc cgaagggtta ctcggtcggt atggatatct agccgtcgag gtaacacgta
     2161 atggccgttg gccagaagaa tcgtcccgcc aggcggtacc acctcggctg cccgataaag
     2221 ctcctggaca gtcctcacac gaatcgcggg cccggcgggt gggggcaaag ggggagctgt
     2281 gcggaggatt gagggaggaa ggggtggcaa gacgagacca ccggcactct cgcccagcgc
     2341 cttttgggac accagggcac agagcaggac aagggaagtg aagaatcgtt tcatggcgca
     2401 tcaatttgat tttttggggg gcactcaagg gtgaatggta cggtgcggtt cacactgcat
     2461 acgcaggcat catcaacacg ttgaggaatt gtgggtcgtt tctcttgtcg caagcattat
     2521 ggagcttgcc gccggaccct gtcaacattc gcggaggcga cgagcgatgc ccgagtggcc
     2581 acgacagcca gtggctgtcc agaatcaacc tcatcaattg gtagagtcaa cccttgcagt
     2641 ggccgtttaa tggatggacg cgggggtgag tcacgtcgca tcaaattgcg agggacgagc
     2701 tcttctgcct ggccggtttc aattactgca aaacggacga agatggtttg acaaactccg
     2761 cccggtaaat ggtcccgccc tgtaaacgga cacggcgttc aaaatgcgtg cgaaaatcca
     2821 tgtcgttttc cgccgggcgt tccggaacag gaaaaggccc caatagagac gtggtgtttt
     2881 ttagcagctc gattgtggtt tggaaatact ccgccacgtc cgtccagaaa tggagtcgtc
     2941 cctccggccg aagggcccgc tgcacattct ttagaaaggt ttcattgagc acgcgccgct
     3001 ttttgtgtcg cttcttccac cagggatcgg gaaaatagac gtgaactgct tgaacgcttt
     3061 ccggcagcaa ccattccgca aagaataatt gagcgtcgcc atggatgacg atagcattcc
     3121 gcacacccaa ctttgccaag cccgccgcgg agaatcgggc gtatttctga gcgatctcca
     3181 aaccgacgaa gtcaacatcc ggagtcccag cggctgcacg acggaggaac aggccctttc
     3241 cacttccgac ctcaacttca agaggagccg accgaccgaa gatcacttcc gcctgaaacg
     3301 gcttggggat attcggcaat tcccgataat gaaaagagag atcgaggttc ggatcgatct
     3361 tacgaagtgc tcgtcggccc atagtcgccg cggagaatat gctaacagga acgcgatcgg
     3421 tgacggaaac agtgagggct gagagcctat tccataatct cccctcgccc gttcggcgag
     3481 agatgggccg ggggtgagtg tggctcgaat cgccgccggt tttctagatc cactccgacg
     3541 atcgcttgcc ctcacccgaa ccctctcccg gaggaagagg gagacttttt gggataggct
     3601 ctaggcaccc gcgtctgcgg aggccgtctc ggctccatta tccctgctgc cctcgatggt
     3661 ctcgggaaac aggggaattc ccaggatgac cccttcacac acgagatttt cccgcacaaa
     3721 gcactggaat tcgcacgtca gcatcgtact gcgaactttc aacaggcggg ctacgatcag
     3781 gaagcggtcc ccgggacgga caacgccgcg aaaacggacg tcctgaagtc ccccaaatcc
     3841 gaccatgggg gccttcatga gtttgtgctt gatggtgtaa tagctggcga gctgggcagc
     3901 cgcttcacac atgatcacgc cgggcatgat aggcattccg ggaaagtgcc cccggaccca
     3961 gaactcgtct gggcctaagt ccttgtatcc ggcgcagatg gcccgctcga gatcttcgta
     4021 gcagatcgcc gtgagttgct ccatctcata gcgctgtggg ttatagcgac ggatttcctc
     4081 aatgtcggcg atgatccggc tcgtatcaat ttcggcgggg tccagaatca atggcttctt
     4141 gggcacaacc agctcctcaa aaaaccaatt ccgcgcaaca cacaacgcag gaccagcccg
     4201 atggcccttg tcaagaccaa cgattgaata tcctaacctc ctaggtcttt accacccagc
     4261 gcttcgcccg cctcgcccgt gagttggcgg gtcggcgtcc gtgattggct ttcttaatat
     4321 gacggtgcgg cttcgccatt agatcacctc atctgccata caacaatgcc tcatttcaga
     4381 tacgatggga cttagaaaca gggccgaaaa ggcctttcaa aactaggcct tctgcccgag
     4441 cgtcaccccc gcggaggcca tcccatcgat cactattcgc gaaatctccg ccgtgtggcc
     4501 ctattccatc gtggcctgac acacccgaga gacaaaacgc cattatggat aggcacaagg
     4561 cccaccccga accataggca accacaagcg gggcaaaatg ataatttacc ttttcgcggg
     4621 ctttctgtga agacgtgaaa acaggcaaaa tgggaaagat cgcctaacaa ctggccatgc
     4681 cttagcgact gtcggccgag gattcggcca agccgagcgt tacgaaaagg acggtgtgca
     4741 tctgcgggat gatcgagacg tcgtgaaaca ggccatttgt gacgtaagcc acaatcgcga
     4801 ctatcgccat caatggaaag acgagatttc cctgtgacac tgccgagcac atgtcacggg
     4861 tccaagccgc caaaaggaag agaaatacca ttaggccgat ggctcctgtt tccacgagga
     4921 tcgagagaaa cacgttatgc tgggtgtatg gctttgccca atggacgggg taagaagcca
     4981 agggattggc gaggtatttt gtctttgccc ggtcgtattg tcctagccca acgccaaaaa
     5041 agggatgatc cttggccatt tcccacgcaa ctagggcaag gatggggcgc aactgggcgg
     5101 atcgggcggc ctcggcaggg ctgaggcctt gatcgcgctt catttcccaa aagaagcctt
     5161 tcacgacaag ggccataaca attaggccaa atccggcggt ggccagccat gcccggcgat
     5221 agccccccgg caccaagtgg tatcccaaaa acagcccagc gagccccgcg gccagccaga
     5281 cactgcgggt gagcgtgcta atgactccta ctgccatgat tccgtggacc aagactaacc
     5341 caggccacca acggcgaggg gcggaccgcc ccagaaatac ccatgcggcc caagccacta
     5401 tcatgatgaa gccattgatg atgggattta gcagggggcc gcgtgcacgt cctaggaatt
     5461 ctccgctcac ggagcgatcc atgatgtaag cgggatagat aagcgaccgc aacccgaaga
     5521 cctcaaacaa tccggtcagg gacaggcaaa cccctacgca gccaagcacc aggaggattc
     5581 cccgctccgc cgacgatgat agatggattt gtcggagggc aaaatagagg ctggccggca
     5641 tgaaataata gaagacccaa cgggcagcgg cacccgtgtg ttccgagctg aattgcatca
     5701 ccgctaacgc gccgagatag gcgacaagca cggccaggaa gaggtcccct cgcgcgattc
     5761 gggtaaaagt tacgccccgg cgaactgtga tgaaaaccgt ccctagcaga acagtcaacc
     5821 aggcgattcg atccaaggtg atggggaccc ctaagctgtc gtcgtgccaa aaagggtgtc
     5881 ccaaccatat cccagccagg agtgtcaaca gcacaagggc caacaagagg ctgtcagccc
     5941 gtgatgttgt ccacagtttg aagggccttc gaacggcacc aatggccgaa gacgaggcga
     6001 tcatgctttc cccccaggat cacgggattg agccataccg gcgagcgcct ggtgtaaggg
     6061 tgactcggcc cagtcctcgg caaccgggat tgctggccag tttgtgggag tgtctgcctg
     6121 cgacgcgcga tctttccaca gaccatccgg tgtcgttggc tggatcgccg ttgaggcgag
     6181 tgccggctgg gcttcggcgc caggagagtc gaatagaggc ccatgagatg tcacatcggc
     6241 ctcagaggga atgcgcgtct ctttcttcgc cgttggtagg accatttcag cttgtacggg
     6301 ttggtcaccg tcttcttcgt gagttggcgc atgtgatgtc acaatcggtt ttgtttccga
     6361 aggcgcgacg ggagttaacc aaagaaagac acatagggca aggaacagcc cgcagacgaa
     6421 tcccccggcc acgatcgtga gtgtcccagg gccaactggg cggttgcccg ttcgcggcgg
     6481 ttcgacggct gtgataagcc gtgctgaccg agccgacgcg gcgcccgcgc gggcgatggc
     6541 caacctgtgt tgggcgtcgc ggacggtttc tgattgagcg cggatggcgg ccatgagttg
     6601 ccgatacttg ggcaggacag ccaagatgtg ttgaaccctg gcttcctcgg tttggcgttt
     6661 ttgttcaaga aattgttttt ggttagccgc cacggcttgc tcgttctcga tcgtttggat
     6721 caccgtaggg acttcctcag atatacggtt tgtcaaatcc tggagggcca gttgggccgc
     6781 aatcacggcc gggtgctcat cggcatactg gccacggagt tcgatgagtt taacctcggc
     6841 atcagtcagg gcctcttgaa agcgtttgag tgtggggtat ttttccagca ttgccggcgg
     6901 aatgctggcc agtgcctcag gggtggccaa cgtcccgcga aggagccgga gtaattcttc
     6961 ttgtagcaca atttgggctt cgagttcatc cagttggtcg tccagttcct caatcgcgga
     7021 tcgcaagagc gattcgtcgg gtttttcttc gtaagcacgt aacgttaggc tgtccagccc
     7081 tagttgtgct tcaagggcag ccaattcttc tttcaagttt tgcaggacat cgttggcgct
     7141 cttttccgtg aacacgagtt cttgaaccgt ggcttcggct ttggccgcct ggacttcgcg
     7201 gaggcggcgg tcagcctgtt cgaccagcac actggccaag cggccggccc gttctggccg
     7261 agcgtcccga accttgagat agaccaattc cgtcgtaccg aagtccgtat cggagggtgg
     7321 cacaatttgc acggagcgac gaagccgttc aatatctcta cgatcgggaa acgaatgcct
     7381 cctgacgggc cgggctggtc caacctcacg gagagtggcc tccaagacat cttgactttg
     7441 gaagactgcc accagcgtat ccaggacatg tcgccgctgt tggtcacttt cgaatcttcc
     7501 tgggattttc gacccttcga gggcttccgc acgaatcacc aagggttggg tggcctccca
     7561 ttcaggcccc ttgagaactc cgtacgtggc aatcgtggca aacaggagaa aactggcaac
     7621 aagggaggtg cggacaggac gttcaataat cagccgtttc gcgagccata tttttccgct
     7681 gaacgaagtt tgcgatgtcg taggcatgct tggttccaca cactgatgga agaaaggtta
     7741 aatccagggc aaaagtagtg tctgaagttg acgatccggc actcgtggcg cggaatccaa
     7801 cgaacagtca accggaaata aaccgcaatc tcttccgcta aaggaatata gttttcgaaa
     7861 acgccgtgat gctaacgcgt tcaccttaaa gcctgtccca aaaag
//
LOCUS       DAOSUT010000180         7890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4673_length_7890_cov_8.982514, whole genome shotgun sequence.
ACCESSION   DAOSUT010000180 DAOSUT010000000
VERSION     DAOSUT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4673_length_7890_cov_8.982514"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1080
                     /locus_tag="PLD05_13510"
     CDS             181..1080
                     /locus_tag="PLD05_13510"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOP78505.1"
                     /translation="MMEAPNNDKLDTTIELITPENIVFRYYLAGPAHRIPAFLIDCIC
                     QFVIFMATSFALVFLTFYFPMLEEIIDFVFLVSIFCIFWLYGGILESVWNGRTFGKWA
                     LGLRVVTVEGLPITPFQALMRNLLRAADLQPFFTGWVGLLTMMLNRRFQRLGDLACKT
                     MVIIDRVSTLQAIPLADHPEIAAVAELLPHRLEFHPSVVRALLLYVHRREWLSWERRN
                     EIAAPLAEAFRKRYSLPSDIPADTIVCAAYQRGVLGSAAQNGQNAVMGSLRRPDADRS
                     SPQASALPFGPLPAETPSSETRN"
     gene            995..2269
                     /locus_tag="PLD05_13515"
     CDS             995..2269
                     /locus_tag="PLD05_13515"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOP78506.1"
                     /translation="MPIALRRRPPPFLLVLYLPKLLLRKLVTDVASVSEPITSWTQSS
                     AGGPSRQEAPSPTWHTGPNHEPPLPILVTGLAGVAGFHAFRWLVARYPGRVWGLRPGN
                     NHHVRGEGILACNIEDRHGLERLFREYRFAAVLDAVGNCALKPCELDPRVAWTINVEG
                     LKNILAAAGREKVRLVRLSVDLVFSGKKGGDYVETDPPDPVTVYGKTMAVAEQILLET
                     DPSACVLRISLPMGRSPNGHAGAIDWIESRFAAGRPATLYLDEVRTPTYVDCLSRVCF
                     EALRRPLSGIYHAGGPTRLSLYQIGQIINAVGGYDPDLLFGIPRRMAGPIPPRAGNVT
                     MNSQKLITALGYQPFLPWPFDERLIPSHRDWHREPIWDDLTRKNKAGYLRYAGYIDKL
                     LCRPGRLPEPDPRDISASRVLGREGQAVHWES"
     gene            2458..3381
                     /locus_tag="PLD05_13520"
     CDS             2458..3381
                     /locus_tag="PLD05_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOP78507.1"
                     /translation="MLRLSVIIPVVRTLARMEETLVSVLEHKPDDCNVIVVLDEAYPD
                     PYGLAGEVTFLQAGPEVSLSQAINLGIEAAKTPLIHVLPAGSCVHAGWAESAMPHFQD
                     SRIGAVIPVTLDQQHPDRILAAGMGYDSAGRLIYLQRGISLRETQLCHRTQVLPHFSG
                     AIFRAASLHALHGADESVGERWVFADLGLRLLRAGAVTVLEPHCWVSSDPATEPASLG
                     YAAARDAEAFFWRWCDRRSTTLARHLALITRETLAAVPERQVWPSVAGRIAGFTQRRH
                     NGRPRPKAFSQDTKPTKSVASDLGIPMARAG"
     gene            complement(3402..4466)
                     /gene="fliM"
                     /locus_tag="PLD05_13525"
     CDS             complement(3402..4466)
                     /gene="fliM"
                     /locus_tag="PLD05_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOP78508.1"
                     /translation="MSSDEVLTQAEIESLLASVGKGKSAPSPTPSPQPAAPSPKTPAA
                     PPSAPPRKSREKIIPYDFKRPERVGKEQMRSLQTLHEGFSRNFAASLSAMLRSMVEVK
                     LTSVDQLTYSEFVFSLDNPTCFNLIKAEPLEGHIILDLNPTILYPMIDRLLGGGREPT
                     PITRRPLSEIERRLVGRITNLFLDELKKAWENILPLQMEVVRVESNPQLVQIVPPNEV
                     VVLVSFELALGEIRGMANLCIPYNTIERISNKLSANSWASYGKRQTTPDAAERIGRLL
                     RTSTVQVVVELAKTHITTEDLIGLRVGDIITTRKDIHTPLVVKVEGIPKFLARPGIFK
                     GHKAICIEEIVTDPTQALTD"
     gene            complement(4768..6042)
                     /locus_tag="PLD05_13530"
     CDS             complement(4768..6042)
                     /locus_tag="PLD05_13530"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOP78509.1"
                     /translation="MANENGFDLREYCLDLAKRAKAASVELARLSGGVKQRWLQRAAR
                     LLKERVIALTEANHKDLAAAPSFGLTEAQIDRLKLTGPRIEAMAKALEEVALLPEPIG
                     EVIWSSVRPNGLLVQKVRVPLGVIFFIYESRPNVTADAAALCVKSGNAVILRGGKEAA
                     NSNRAIVDIMLEAGADVGLPVDAIQLVATPDREAVGHLLNLPEYIDLTIPRGGESLIR
                     RVTAEAKMPVMKHFAGNCHVYIDASADPDMAERIVINAKCQRMGVCNAAESLVFHRAV
                     ADTLLPRILQALSERGIEIRGDEETCARFPAAKPAIEDDYFAEYLGPIISVKVVDDLD
                     AAIEHINHYSSKHTEAIVTRDLLAAQEFAQRIDSSAVMINASTRFNDGGEFGLGAEIG
                     ISTDKFHARGPCGINELTSYKYVVIGNGQVRE"
     assembly_gap    6550..6649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6911..>7890
                     /locus_tag="PLD05_13535"
     CDS             6911..>7890
                     /locus_tag="PLD05_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78510.1"
                     /translation="MVSYQSKRRDDSSRRGWVLKGPLFVLGMMIVGIPVVWSFLPKTL
                     WQGSHEKPLTTTVTRGEFINQVVERGDVESASNVEIRCEVETQGSGMMIIRIVPEGTY
                     VQKGDFLVELDSSTLRDQLVQQQIKVANERALLIQAQRALDTAIIAKQEYLEGQYRQQ
                     RLAIEIDNFIAQENRRRAEDYLKYSQSLAQKGYITQAQLEADQFALEKAIKEMESART
                     KLDVLERFTKPKYLMQLDADIETARAKLEAQQHSLELQEQELKKIQDQIAKCIITAPE
                     SGQVVYANQIGVRGQTDIIIQEGVRVRQYQTIIRLPDPKRMQVKTKVNES"
BASE COUNT         1633 a         2270 c         2177 g         1710 t
ORIGIN      
        1 acccctctcc cgccgaacgg gagaggggag gttgtgggat gggctcttgc ccgacctgtg
       61 cctttgacct cgatagaccg aacagattcc ttaacccttt aataatttca gcgtcgaaaa
      121 catcgatttg gcgacgcaaa ctactggctg agaatcttta aaaaggctac catagtcccg
      181 atgatggaag cccctaataa tgacaaactt gataccacca ttgagctaat cactccggaa
      241 aacattgtct ttcgttatta tctcgccggt ccggcacatc ggattccggc gtttcttatc
      301 gactgcatat gtcaatttgt catcttcatg gcaacctcgt tcgcgctggt atttctcacg
      361 ttttactttc ccatgctgga ggaaattatc gacttcgttt ttctcgtctc cattttttgt
      421 attttttggc tttatggggg gattctggaa agtgtttgga atggccggac gttcggcaaa
      481 tgggcccttg gcctgcgtgt cgtcacggtg gagggcttac ccatcacgcc ctttcaggcg
      541 ttgatgagaa acttgctccg cgcggcagac ttgcaaccat ttttcacggg atgggtgggg
      601 cttcttacga tgatgctcaa tcgccgattc caaaggctcg gtgacctagc gtgcaaaacg
      661 atggtgatta tcgaccgcgt ctctacgctt caggccattc ctttggccga tcaccccgag
      721 attgcagccg tggcggagct tcttccacat cggttggagt ttcaccccag cgttgtccgg
      781 gcactacttt tgtacgtcca taggcgggag tggctttctt gggagcgtcg caatgaaatc
      841 gcggccccat tggccgaggc cttccgaaag cggtacagcc tgccctctga catccccgcc
      901 gataccatcg tctgtgccgc ctatcaaagg ggcgtcttgg ggagtgctgc ccagaatggc
      961 caaaatgctg tcatggggtc acttcgacgg ccggatgccg atcgctcttc gccgcaggcc
     1021 tccgcccttc cttttggtcc tttacctgcc gaaactcctt cttcggaaac tcgtaactga
     1081 cgtggcttcc gtttcagaac ccatcacctc atggacgcag tcgagtgcgg gaggaccttc
     1141 ccgccaggaa gccccttcgc ccacttggca cacggggcct aatcacgagc ctccgcttcc
     1201 catcctggta acgggcttgg ccggggtggc tgggtttcat gcctttcgct ggttggtcgc
     1261 ccgttatccg ggacgagttt ggggactacg tccgggcaac aatcaccacg tgcggggaga
     1321 agggattctg gcctgtaaca tcgaggatcg ccacggcctg gagcggcttt tccgcgagta
     1381 tcgttttgcg gcggttctgg acgccgtcgg caattgcgcc cttaagcctt gtgaactcga
     1441 tccacgtgtg gcttggacga tcaacgtcga gggattaaag aacatcctgg ccgccgccgg
     1501 ccgtgaaaag gtccggctgg ttcgtctttc tgtggatttg gtcttctccg gcaagaaagg
     1561 cggagactac gtggaaaccg atcctcccga ccccgtcacg gtttacggta agaccatggc
     1621 tgtggccgaa caaatcctcc tcgaaaccga tcccagcgcg tgtgtcctga ggatttcttt
     1681 accgatggga cgtagcccca acggacatgc cggggccatc gattggatcg aatcccgctt
     1741 tgcggcaggc cgaccagcga ccctctatct cgatgaagtc cgcactccca cctacgtcga
     1801 ttgtctcagc cgcgtctgct tcgaggccct ccgcaggccc ctgtcgggga tctaccatgc
     1861 gggcggtccg acgcgactca gtctctatca aatcggacaa atcattaacg ccgtcggtgg
     1921 gtatgaccct gatctgctat ttggcatacc tcggagaatg gcggggccca ttccaccacg
     1981 ggcaggaaac gtcacgatga atagccaaaa gctgatcacc gccctgggat atcagccgtt
     2041 tttgccttgg ccattcgatg agcggctcat cccttctcat cgggattggc accgcgagcc
     2101 aatttgggat gatttaacca gaaaaaacaa ggcagggtat ttgcgttacg cgggctatat
     2161 agataaactg ctgtgtcggc cgggaaggct cccggagcca gaccctcggg acatctcagc
     2221 gtctcgagtg ttaggaaggg aaggccaggc ggttcattgg gaatcctaat aaactcctgg
     2281 aattaaatgg ccgacataaa atggcgcggg cagattgtgc caggtcaaag gagggagctc
     2341 ctacgccttg ccggaccccc aaaatcgtga gaacagccac gatcttgacg cgaactccaa
     2401 aaagcaacac tgtctagttg gcatctttga gggtttgttt ctgacgaggg tttacccgtg
     2461 ttgcgacttt cggtaattat ccccgtcgtc cgcacactcg cgaggatgga agaaacgctt
     2521 gtatcggtct tggaacacaa accagatgac tgcaatgtca ttgtggtgct cgacgaggcg
     2581 tatcccgacc cctacggatt ggcgggggaa gtaacctttc tccaggcggg accggaagtc
     2641 agtctctccc aggcaattaa cctaggtatt gaggcggcca aaaccccgtt gatccatgtc
     2701 ctgcccgccg gcagttgtgt tcatgctggc tgggcggagt cggccatgcc gcacttccag
     2761 gattcccgaa ttggggccgt tatcccagta accctggacc agcagcatcc cgatcgcatc
     2821 ttggcggcgg gaatgggtta cgacagtgcc ggccgattga tttatctcca acgcggcatc
     2881 tccctgcgtg agactcaatt gtgtcatcgg acccaagtac tcccgcattt ctcgggggcg
     2941 attttccgag cggccagcct ccacgcactc cacggggccg acgagtccgt cggggagcgt
     3001 tgggtgtttg ccgatctcgg gttgcggctg ttgcgggctg gggcggtcac ggtgctggaa
     3061 ccgcattgtt gggtatccag tgacccagcg acggaaccag cctcgctggg atatgcggca
     3121 gctcgagacg cagaggcctt cttctggcgc tggtgcgacc gccgcagcac gacgctcgcg
     3181 cggcacctgg cgcttatcac ccgcgagacc ttggccgccg tcccggagcg tcaggtgtgg
     3241 ccctccgtgg cgggtcgcat cgccggattc acgcagcgcc gccacaatgg tcggccccgc
     3301 ccaaaggcct tctcacagga tacgaagccg accaagtccg tagcctcgga cctgggaatc
     3361 cccatggccc gcgcgggttg aattcgcccc ttcgctgaga ctcagtcggt aagcgcctgg
     3421 gtgggatcgg tgacgatctc ctcgatgcaa atcgccttgt gccccttgaa aatccccggc
     3481 cgagccaaaa acttgggaat cccttccacc ttaacgacca acggcgtgtg aatatccttg
     3541 cgcgtggtga tgatgtcgcc cacgcggaga ccaatcaggt cctcggtggt gatgtgcgtc
     3601 ttggccagct ccaccaccac ttgaacggtc gaggtccgca aaagtcgccc aatccgttcg
     3661 gcggcatcag gcgtggtctg tcgcttgcca taggaggccc aactgttggc cgaaagcttg
     3721 ttggaaatcc gttcaatggt gttgtagggg atacacaaat tggccatgcc acggatttcc
     3781 cctagggcca attcaaagct gacgagcacc acaacctcgt tgggcgggac aatttggaca
     3841 agctgaggat tactctcgac gcggacgacc tccatttgca gcggcaggat gttttcccat
     3901 gcctttttca attcatcgag aaacaggttt gtgatccgcc ccaccagtcg tcgttcgatc
     3961 tcggaaagcg gccgccgcgt gatcggggtc ggctctcggc caccgcccag cagccgatcg
     4021 atcatcgggt aaaggatggt gggattcaaa tccaggatga tatggccttc cagcggctcg
     4081 gccttaatga ggttgaagca cgtgggattg tccaaactga acacgaactc gctgtaggtc
     4141 aattgatcca cgctagtcag cttcacctcg accatggatc ggagcatggc cgagaggctg
     4201 gccgcgaagt ttcggctgaa cccctcgtgc agcgtctgga gcgagcgcat ttgctctttt
     4261 cccacgcgct ccggacgctt gaaatcatag ggaatgatct tctcccgact tttgcgcggc
     4321 ggtgcagagg gcggcgccgc cggggtctta ggactggggg ccgctggttg cggcgacggc
     4381 gtgggagaag gtgcactctt gcccttgcct accgatgcga gcagactttc aatttccgcc
     4441 tgtgtcagca cttcgtcgga tgacatgcga tcctccgcaa ctcaggcaac cgtgccactg
     4501 tctccgaact cactcatccc cggtgcatcc tcccaaggat attttatttg gagcctatcc
     4561 cataatttcc ccctcacccg ttcggcgggc gaggggtctg gggtgaggct ggctcgaacc
     4621 gctgcccggt tttcccgatc gactccgacc ctcgcttgcc ctcaccccag ccctctcccg
     4681 gggggagagg gggacttttc gaaacagact cccatccgtt cgctcggcga gacatcgcca
     4741 gcgtgggcaa acaccgatta ctgcccctca ttcccgaact tgaccgttac ctatcaccac
     4801 gtacttgtag ctagtcaact cgttgattcc gcatggacca cgggcgtgaa acttatctgt
     4861 actgattccg atctccgcgc caaggccaaa ctcaccgcca tcattgaatc gcgtgctggc
     4921 attgatcatg acggcggaac tgtcaatccg ctgcgcgaat tcttgggcgg ccaggaggtc
     4981 ccgcgtcaca atggcctccg tgtgcttgga actgtaatgg ttgatgtgct caatggcggc
     5041 gtcgagatcg tcaaccacct ttaccgaaat gatgggcccc aggtattccg caaaatagtc
     5101 gtcctcgatg gcgggtttgg cggcaggaaa tcgggcacag gtctcctcat cgccacggat
     5161 ttcgatcccc cgttcgctca gggcctgcaa aatccgcggt agcaacgtgt cggccactgc
     5221 ccgatgaaag accaacgatt ccgcggcatt gcacacaccc atccgctggc atttggcatt
     5281 gatgacaatc cgctccgcca tgtcgggatc ggccgaggca tcaatataga cgtggcaatt
     5341 ccccgcgaaa tgcttcatga cgggcatctt ggcctcggca gtcacgcgac ggatcaggct
     5401 ttctccaccg cgcggaatgg tcaaatcgat gtactccggg agattcaaca ggtgccccac
     5461 cgcttcccga tcgggagttg ccacaagttg aatcgcatcg acgggcaaac ctacgtcggc
     5521 cccggcttca agcatgatat cgacaatcgc ccggtttgag ttggcagctt cttttccccc
     5581 gcggagaatc acggcgttgc cgctcttcac acaaagggct gccgcatcgg ccgtgacatt
     5641 gggccgcgat tcatagatga aaaaaatcac ccccaacggt acgcgaactt tttgaaccaa
     5701 caaaccgttg ggacggaccg acgaccaaat cacctcgcca attggctctg ggaggagggc
     5761 cacttcctcc agggccttag ccatcgcttc gattcgtggc ccagtcagtt ttagccgatc
     5821 aatctgagcc tctgtcaagc caaaggaggg cgcagcagcc aggtccttat gattggcctc
     5881 cgtaagggcg atcacgcgtt ctttgagaag ccgcgcagct cgctggagcc accgttgctt
     5941 gaccccaccg ctgagacgtg ccaactctac cgacgccgcc ttggcccgtt tcgcaaggtc
     6001 caaacagtac tctcgaagat caaacccgtt ctcgttcgcc attgccgtct ctcaagttca
     6061 cgcagtacgt ggtctgctca aatcaccatc accttacctg acacagccac cacccggccg
     6121 gatcaaccgt ggggtcgtgt cgccattgcg caggttaccc cctgtcccaa agagaatggg
     6181 caaccttgag gaagtatggc gattttccgc caggacgtca atcgacattg cgctcgcttg
     6241 ccacctcgcc cagggtcagc tatcacagga agaccctcaa gcttcccttc ggggccgttc
     6301 cggtggcgcg ataagccact catcgaggta gtgtctcatg cacaaatagg ggtgataaag
     6361 gagcatccag gggccggcaa gccgcataac ttggcggatt ttttctctca tcgcgggccg
     6421 gtaacagtgg atagggcaac gagcacaggt gggcttgttc gacccgaaag gacaatgttc
     6481 caaacgacag gcggcataat ggagaagctc ttggcaatca ggacatactt tcccctgata
     6541 ggggtgcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gaccgcctcg
     6661 gcgaatcggc tttcccgcca ttgaatgagc ccgcacgaac ggattcccac cacatcggaa
     6721 acaattgcgc aaagtcgttg ccagcgagga aataaactta ggcagttgcc tttcggcccc
     6781 gattgtccgt agcagggcga atttccgtcc taagcgaggg atttccgctt ttttgggcgg
     6841 aagcggaggg ctcgcctgag gaaaaaaggg gctcgccgat gttgggggat ttcggtgaag
     6901 gagaccttcc atggttagtt atcagtccaa gcgtcgtgat gattcctccc ggcggggttg
     6961 ggttttgaaa ggccccttgt ttgttctcgg gatgatgatt gtgggaatcc ccgtcgtttg
     7021 gtccttcttg cccaagaccc tttggcaggg gtcccacgag aagcctctga cgaccaccgt
     7081 cacccgggga gagttcatca atcaggtggt cgaacgaggc gatgtggaaa gtgccagtaa
     7141 cgtggaaatc cgctgtgagg tggaaaccca gggcagcggg atgatgatca tccgcatcgt
     7201 cccggaagga acttatgtcc aaaagggtga ctttttggtg gagctggact cctcaacgct
     7261 tcgcgatcag ttggttcagc agcaaattaa ggtcgccaat gagagggcac tgctgattca
     7321 ggcccaaaga gcgctggaca cggccatcat cgctaagcag gagtacctgg aagggcagta
     7381 ccgccagcag agacttgcca ttgaaatcga caacttcatt gcccaagaaa accgccgtcg
     7441 ggcagaagat tatcttaaat acagtcagag cctcgcgcaa aagggctaca tcacgcaggc
     7501 ccaattagaa gccgaccaat tcgctttgga aaaggctatc aaggagatgg aatctgcccg
     7561 gactaaactc gatgtcctgg aacggttcac taaacccaag tacctcatgc aacttgacgc
     7621 tgacattgag acggctcggg caaaattgga ggcccagcag cactccttag agttgcagga
     7681 acaggagctc aagaaaatcc aagatcaaat tgcaaaatgc atcatcacgg cccctgagtc
     7741 tgggcaggtg gtttatgcca accaaatcgg cgtgcggggt cagacggaca ttatcattca
     7801 agaaggtgta cgagtcaggc agtaccaaac gatcatccgc cttcccgatc ccaagaggat
     7861 gcaggtcaaa accaaggtca atgaatcgaa
//
LOCUS       DAOSUT010000181         7784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4736_length_7784_cov_9.424376, whole genome shotgun sequence.
ACCESSION   DAOSUT010000181 DAOSUT010000000
VERSION     DAOSUT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_7784_cov_9.424376"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PLD05_13540"
     CDS             complement(<1..1280)
                     /locus_tag="PLD05_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOP78511.1"
                     /translation="MPKFQFEAMDATGQEIRDVIEAATQEEAEQTIRQMGYYITRIAP
                     QKEKKEKAQKAKSRKTFSIGRVNAKTLTTFTKQLSVLQDAGLPILRSLKILEAQAKPS
                     LFGSLKNCLIDVIEEIEGGATLSEAMAKAPKAFDRLYVNMVKAGEAGGALEVILRRLA
                     EFQERALWLRRKVKGALVYPIAVIVFACLILTFIMIKIVPQFQKIFEEFETDLPVPTQ
                     ILITASQTVIHWWFLLPGVPLGIWLFVKLTTMFPYAKFGLDLFKLKLPIFGQLVEKNI
                     LARTTRTLGTLVASGVPILEALTITRDTSTNAVFERLYQRVLDAIREGETIAKPLKDN
                     SRPGFHPVTAWWFFFFLGGPIGLLLYLTRWNNPIIEDIAINMVDVGEETGELDTMLYK
                     IADNYDEEVAIITESLLSLFEPILIIVLGVMVGF"
     gene            complement(1411..3126)
                     /locus_tag="PLD05_13545"
     CDS             complement(1411..3126)
                     /locus_tag="PLD05_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOP78512.1"
                     /translation="MALRRIGQILVDLGFITESQVELVLEEQKQHKGELFGQIAIRMG
                     LINEDQLAQALAEQFNLQVVQLNELVIPPEVLNRITEPMAQLYKIIPISFHDNTLTIA
                     MCEPQKISVVDELRSFLGYDIRPVVTTERQINQALQRYYSGTESVEKIIDDLEKDEEL
                     AKAAQALDSEGPIDLSSVEALAESAPVRRLLNMVLLMAIKEHASDIHFEPFENEFRIR
                     IRSDGVLYEMVPPPRHLAFAITTRIKVMANLDIAERRLPQDGKIRLTVGGHPVDLRVS
                     VLPTMFGESCVLRVLDKSVVMLDLNQVGMDPTTLANFRDVIHRPHGIVLVTGPTGSGK
                     TTTLYAALNELNRIEDKIITTEDPVEYTIDGLVQIPIDPDIGNTFANLLRAILRHDPD
                     IILVGEIRDLETAEIAVQAALTGHMVFSTLHTNDAPSSIARLRDMGVPPFLITATVEA
                     ILAQRLVRKICPNCRELTVPSTEALADLEMTPDDVVGKPFYRGRGCAACNNTGFKGRT
                     GIFEFMPITDEIRELIHRGASTEELRDAAQRAGMKTLRQAGLEKIYEGVTTVEEVVRE
                     TTIEA"
     gene            complement(3222..4361)
                     /locus_tag="PLD05_13550"
     CDS             complement(3222..4361)
                     /locus_tag="PLD05_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOP78513.1"
                     /translation="MSQLQIDKLLNTVVARKASDLHISVGQPPALRIDGRIVRLETKV
                     LEPEDTVALMKSITPERCQRELQEVGGADFGFAFGDKCRFRVSVFKQKGYVGMVLRQI
                     PNRLMNFEELGTPPVFKELIMRPRGLILVTGPTGSGKTTTLAAAIDYLNDNCDHHIIT
                     IEDPIEFYHSHKRCVVNQREIGVDVPSFAEAIRRALRQDPDIILVGEMRDLETIEAAI
                     TAAETGHIVFGTLHTTGAQGTVNRIIDAFPTNQQEQIRTQLSVSLIGVLSQQLLPRIG
                     GGRVAAYEMLVVTPAISNLIRENKVYRINSAIQTGHKLGMQLLDDHLFRLWRDGICEK
                     KEVLLRANSPDELAARIAAAERGILEDQEEAQKRLEREMAQQAKR"
     gene            complement(4531..6249)
                     /locus_tag="PLD05_13555"
     CDS             complement(4531..6249)
                     /locus_tag="PLD05_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOP78514.1"
                     /translation="MAVKRRVDYTDLLIRKGIISADQLEEAKQLAAQSGMKLPDAIIR
                     LGYASGEEVMQVMAEQHGLEYIDLNRISIPPNVVELVPESVARENVVIPIAEDDGQLR
                     VAVCDPDDFATFDKLRFILNRRIEICLAPREMILEAINRYYGQTSGESQDSVLSEVTD
                     TAIDFTQVETDMGSIQDADETSAPVVRLVHLIISEAVQLRASDIHIEPFEDRVRIRYR
                     IDGYLIERDNAPRRLLGAILSRIKILARMDIAERRRPQDGRIKMNIGEKQLDLRVSVL
                     PSTWGQAVVMRILDKDNIKIGLRQLGFSEEDYQKFRQLIKRPNGIILVTGPTGSGKTT
                     TLYAALNELNTPDRKIITAEDPVEYYLPGINQVEIRHEIGLDFARVIRAMLRQAPNVI
                     LVGEMRDLETAQMGIQASLTGHLVFSTLHTNDAPSSITRLIDMGVPAYLVASSVVAIM
                     AQRLVRVVCEQCKQPYQPSDAVLEMAGITPEMAATATFYRGKGCSRCNGTGYRGRMGI
                     FELMIMSPKIREMTFRQAPVADIRRVAIQEGMRTLYMDGLQKVLRGRTTLEELLSVAR
                     PEEELR"
     gene            6567..7625
                     /locus_tag="PLD05_13560"
     CDS             6567..7625
                     /locus_tag="PLD05_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78515.1"
                     /translation="MAYLLGLLLALHLTSLVVMGDTANTTVSFDEKKLLPPWPDESPL
                     LEGPELTLPEIETPTTTSWVWISSQDMEPGELSGPTFENFLGNSGPADGASAEPTGDS
                     LLIPEDQVFAPDAVSVRGATVATSGGVRCCSEDPSGCRPRRGIVRGLFADLQNAGRWQ
                     RWRSEPGSAGVFLGYGWGTELVHNWLEERDGIWGGLRLGWDCSEQYGVETRLSFGTLD
                     LWDHPDAVAAGSGLGLNGSRDRWLRAVEWDAVLLYYPTENDPWRPYVFWGVGLTRIDF
                     ADFVGTSYEGTYFAMPIGLGLKFRPKSGPIMRFEFVDQIVFPSRFNTTHHFAFTVGLE
                     MRFGARRKIYWPWESTRW"
BASE COUNT         1596 a         2226 c         2149 g         1813 t
ORIGIN      
        1 atgaagccaa ccatcacccc caacacaata atcaggatgg gctcaaacaa gctcaaaagg
       61 ctttctgtga tgatggccac ctcttcgtcg tagttgtcgg cgatcttgta caacatggtg
      121 tccaactcac cggtttcctc gccgacatcc accatgttaa tggcaatgtc ctcgatgatg
      181 ggattattcc aacgcgtcaa gtagaggagc agcccaatag gtcctcccag gaagaagaag
      241 aaccaccacg ccgtcaccgg atgaaagcct ggtcgagaat tatccttaag cggcttggcg
      301 atggtttcac cttcccgaat ggcatcgagt acccgctgat atagccgctc aaaaacggca
      361 ttggtggacg tatcccgcgt aatcgtgagg gcctccaaga tgggcacacc actggccacc
      421 agggttccca aggttcgcgt ggtacgtgcc aagatgttct tctccacaag ttgtccaaag
      481 atcggcaact tgagtttaaa caggtccaag ccaaatttgg cgtagggaaa catcgtcgtc
      541 aacttgacaa agagccagat tcccagcgga acacccggca ggaggaacca ccaatggatg
      601 accgtttgag aagccgtgat aagaatctgc gtggggaccg gcagatcggt ctcgaactct
      661 tcgaaaatct tttggaattg cggcacgatc ttgatcatga tgaaggtcaa gatcaagcac
      721 gcaaacacaa tgacggcgat aggatagacc aaggcgcctt tgaccttccg ccggagccag
      781 agggcccgtt cttggaactc tgccagacgc cgcagaatga cttccaaagc accaccggcc
      841 tcgccagctt tcaccatgtt cacataaagc cggtcaaagg ccttgggggc cttagccatc
      901 gcttcagaga gcgtagcccc gccctcaatc tcctcgatca cgtcgatcaa gcagtttttt
      961 aggctcccaa acaacgaggg cttagcctgg gcctcgagaa tcttcaaact ccgcaggatg
     1021 ggcaagccgg catcctggag caccgaaagc tgcttggtga aggtcgtaag ggttttggca
     1081 ttgaccctac caatggaaaa ggttttacgg ctcttggcct tttgggcctt ttcctttttt
     1141 tccttctgag gagcgatccg cgtgatgtaa taccccatct ggcggatcgt ctgttcggct
     1201 tcctcctgcg ttgccgcctc aatgacgtcc cggatctctt gtccggtggc gtccatcgct
     1261 tcgaattgga acttgggcat ggcctgctcc tcactccgct caggctcagg caatcacgat
     1321 cactcgatcg ttccgccgtc aactaccgtt tttgtgaatc ggtctaggct ccgttgaatt
     1381 atttcaatcc gaaggtcggc gtcgaaaaaa tcacgcctcg atggtggttt cgcggacaac
     1441 ttcttcgacg gtcgtcacgc cttcgtagat cttctccaag cctgcctgac gcaaggtctt
     1501 catcccggct cgctgggcgg catcccgcaa ctcctccgtg gaggccccgc gatggatcag
     1561 ttcccgaatc tcgtccgtga tcggcatgaa ctcgaaaatc cccgtccggc ctttgaagcc
     1621 cgtgttgtta caggcggcac aaccccgacc tcggtagaat ggcttaccca ccacgtcgtc
     1681 gggagtcatt tccaagtcgg ccaaggcttc cgtgctcggg actgttaatt cccggcaatt
     1741 tgggcaaatc ttgcggacca agcgttgtgc caagatggct tccactgtgg ccgtgataag
     1801 gaatggcggg acccccatgt cgcggagccg ggcaatggaa ctgggtgcat cgttcgtgtg
     1861 gagggtgctg aacaccatgt gacccgtcaa cgccgcctgc acggcgattt cggccgtctc
     1921 gaggtcccgg atttcgccga cgagaataat gtcgggatcg tgccggagga ttgcccgcag
     1981 cagattagca aaagtattgc caatgtcagg gtcgatcggg atctgcacga ggccgtcaat
     2041 ggtgtattcg acggggtcct cggtagtaat aatcttgtcc tcgattctat tcaactcgtt
     2101 gagggccgca taaagtgtcg tcgtcttgcc ggaacccgtc ggccccgtaa ccaaaacgat
     2161 accgtgcggg cgatgaatga catcccggaa gttggccagt gtggttgggt ccatacccac
     2221 ctggttgagg tccaacatga cgaccgattt gtctagcacc cgcaacacac aactttcacc
     2281 gaacatggtg ggcaggacgc tcacccgcaa atccaccggg tgtcctccga cggtgaggcg
     2341 aatcttgccg tcttgcggaa gacgtcgctc ggcgatgtcg aggttggcca tcaccttgat
     2401 ccgtgtcgtg atggcgaagg ccaggtgccg cgggggcgga accatctcgt aaaggacgcc
     2461 gtccgagcgg atgcgaatgc ggaattcatt ctcgaacggc tcgaaatgga tgtcgctggc
     2521 gtgctccttg atggccatca ggaggaccat gttcagcagt cgccggaccg gggcactctc
     2581 ggccaaggcc tcgacactgc tcaaatcaat cgggccttcc gagtcgaggg cctgggcggc
     2641 cttcgccagt tcttcgtctt tttccaggtc gtcgataatc ttctcgacgc tttcggtacc
     2701 cgaatagtac cgttggaggg cctggttaat ttggcgctcc gtggtcacaa cggggcgaat
     2761 atcatagccc aggaacgacc gcagctcatc gaccaccgag atcttttgcg gttcgcacat
     2821 ggcgatcgtg agtgtgttgt cgtgaaaaga aatggggatg atcttgtaaa gctgggccat
     2881 cggctcggtg atccgattga ggacctccgg cgggatcacc aattcattca actgaacgac
     2941 ctggaggttg aactgctcgg cgagggcctg ggccaattgg tcctcgttga ttaagcccat
     3001 gcggatcgca atttggccga aaagctcgcc cttgtgctgt ttttgttctt ccagcacaag
     3061 ttccacctgg gactccgtga tgaagcccag gtcaactaga atttgtccaa tccgacgaag
     3121 agccatccgc gcgttcccgt tgtttcaatc tcaggttctc gataatcttt ccgcgctttc
     3181 tatcatactc agactgctgc gtttctccca agtgcccgtt tttatcgttt tgcctgctgg
     3241 gccatctctc gttcgagccg tttttgggcc tcctcttgat cttcgaggat tccccgctcg
     3301 gcagcggcga ttcgggcagc tagctcatct gggctatttg cccgaagtaa aacttctttc
     3361 ttttcgcata tcccatcccg ccacagccgg aacaggtgat catccaaaag ttgcatgccc
     3421 agcttatgtc cggtctggat cgccgagtta attcggtaaa ctttgttctc acggataagg
     3481 ttggagatag ccggggtaac gacgagcatc tcgtaggcag cgacacgccc tcccccaatc
     3541 cgtggtagca attgctgcga gaggacgccg atcagcgaca ctgaaagttg ggtccggatt
     3601 tgttcctgtt ggttcgtggg gaatgcgtcg atgatacggt tcacagttcc ctgcgccccg
     3661 gtggtgtgca gcgttccaaa cacgatgtgc ccggtttcgg ccgccgtgat cgcggcttcg
     3721 atggtttcca ggtcgcgcat ttcaccgacg aggatgatgt ccgggtcttg gcgcaaggcc
     3781 cggcggatag cctcggcgaa actgggcacg tcaaccccaa tctcacgctg gttgacgaca
     3841 caccgcttgt ggctgtgata aaactcgatc ggatcttcga ttgtgatgat gtggtgatcg
     3901 cagttatcat tgaggtaatc aatggcagcc gccaaggtgg ttgtctttcc agacccagtg
     3961 gggcctgtga cgaggatcaa ccccctgggc cgcatgatca gctctttgaa gacaggcggc
     4021 gtccccaact cctcaaagtt catcagtcga ttggggattt gccgcaaaac catccccaca
     4081 tagccctttt gcttgaaaac cgagacacga aagcggcact tgtcaccgaa cgcgaacccg
     4141 aagtcggccc ccccgacctc ctggagttcc cgttggcacc gctccggcgt gatgcttttc
     4201 atgagtgcca ccgtatcttc cggttccaaa accttggttt cgagccggac gatccggcca
     4261 tcaatccgta aggcgggagg ctgacccacc gaaatgtgta agtcgcttgc ttttcgagca
     4321 acgacggtat tgagcagctt gtcaatctgt aattgcgaca tactgtccgt ccaaaccccg
     4381 atgctttgaa gaccagaaac gctaactatt taccccatct cccgcataat tatcgcgatt
     4441 gatgtcctca ccaaatgggc cttaccggct aacagaaccc cagtggcatc ccaatagcaa
     4501 caaccgggaa aggcgtcgcc gttggaatac tcagcggagc tcctcttccg gtcgggcaac
     4561 tgacaacaac tcttcgagag ttgtccttcc ccgcaacacc ttttgcagac cgtccatgta
     4621 gagggtcctc ataccctcct ggattgctac ccgccggatg tcggcgacgg gtgcctgacg
     4681 gaaggtcatt tcccgaatct ttggcgacat gatcatcaat tcaaaaatgc ccatgcgtcc
     4741 ccggtagccc gtcccgttgc accggctgca tcctttgcca cgatagaacg ttgccgtggc
     4801 agccatctcg ggggtaatcc ctgccatttc caaaacggcg tcgcttggct ggtaaggctg
     4861 cttgcactgt tcgcacacca cccgaacgag gcgctgcgcc atgatcgcca cgacgctgct
     4921 cgccacgagg taggcgggga cgcccatatc gatcaggcgt gtaatagaac tgggcgcatc
     4981 gttcgtatgt agcgtactaa aaacgaggtg tcccgtcaaa gaggcttgga ttcccatctg
     5041 cgcagtttcc aggtcacgca tctcgcctac caggatcacg ttcggggctt gtcgcaacat
     5101 cgcccgaatg acccgggcga agtccagccc gatctcgtgc cggatctcaa cctggttgat
     5161 cccggggagg tagtactcca cgggatcctc tgcggtgatg atcttgcgat ccggtgtgtt
     5221 tagctcgttc aaggccgcat agagggttgt cgtcttcccc gaaccggtgg gtcccgtcac
     5281 caggatgatg ccgttcgggc gcttaataag ctggcgaaac ttttggtaat cttcttcaga
     5341 gaagcccaac tgccgcagac cgattttgat attgtctttg tccaaaatcc gcatcacgac
     5401 ggcctgcccc cacgtgctgg ggaggacact tacccggagg tcaagctgct tttctccgat
     5461 attcatcttg atccgaccgt cttgcggccg gcgacgctcg gcgatatcca ttcgggccaa
     5521 aatcttgata cgcgatagaa tggcccccag cagccggcgg ggggcattat cccgctcaat
     5581 gaggtatccg tcgatccgat atcggatgcg gacacggtct tcgaacggct cgatatgaat
     5641 gtccgacgcc cgcaattgga cggcttccga gatgatgaga tggaccagcc ggaccaccgg
     5701 ggcgctggtt tcatccgcat cctggatgct gcccatgtcg gtttcgactt gggtgaagtc
     5761 gatcgccgtg tcggtaactt cgctcaacac ggagtcttgg ctttcgccgg aggtctggcc
     5821 gtagtaacgg ttgatcgcct ccaagatcat ttcgcgaggt gccaggcaga tttcgatgcg
     5881 gcggttcagg ataaaccgga gtttgtcaaa agtggcgaag tcatcgggat cacacactgc
     5941 cacccgcaat tggccatcat cttcggcgat tgggatgacc acgttttccc gggcaaccga
     6001 ctcgggtacc aactccacca cgttcggagg aatagagata cggttgagat cgatatactc
     6061 caggccatgc tgttccgcca tgacctgcat cacttcctcc cctgacgcat accccaatcg
     6121 gataatcgca tcggggagtt tcatcccaga ctgggcggcc aactgcttgg cttcctccaa
     6181 ttggtcggcg ctaatgatcc ctttgcggat gaggagatcc gtgtaatcga cgcggcgttt
     6241 gacggccatg gaatccgcct cccatggttg gacaaggatt tgttttgctc acctataaca
     6301 tatccccaat tccacagtac ccctgatcgc gtcacctgtc aacaacgcat gttgacagaa
     6361 tgcaatattt tcccgataat cccccggctt ttgactgccg ttccgtttcc cgttggacgc
     6421 tcagcggaat ttcggttgtg caccaagtgc catccaatca tctcccggcc gcgcgtttga
     6481 agcgctgggc acggaagcga ttatgtgttc cgttcccaag tgcgatcgtg ctgtttcttc
     6541 gcaggtcgag cagacgatgg ccgtggatgg cttatttgct tgggctactg ctcgcactcc
     6601 atcttaccag tcttgtggtg atgggagata ccgccaacac cacggtttca ttcgatgaga
     6661 agaaacttct cccgccctgg cctgacgaat ctcccttgtt agagggaccc gagctgactt
     6721 tgccagaaat cgagacaccg acaacaacaa gttgggtctg gatttctagc caggatatgg
     6781 aacccggcga actttcgggc ccgaccttcg agaactttct cggcaacagc ggaccggccg
     6841 atggagcttc agccgaaccc acaggggaca gcctcctgat tcctgaggat caagtgttcg
     6901 ccccggatgc cgtatcggtt cgtggtgcga ccgtcgcgac ctctgggggc gttaggtgtt
     6961 gctcggaaga tccctctgga tgccgacctc gtcgagggat tgtgcgcggt ctgttcgcag
     7021 acctgcaaaa cgccggacga tggcagcgct ggcgcagtga accgggcagc gccggtgtgt
     7081 ttttgggcta cggttggggg accgaactgg tccacaactg gctggaggaa cgagacggca
     7141 tttggggcgg actccggctt ggctgggatt gttccgaaca atatggggtc gagacacgcc
     7201 tttccttcgg aactctggac ctttgggatc atcccgatgc ggtggctgcg ggcagtggac
     7261 ttgggcttaa cggcagccgg gaccgttggc ttcgggcggt ggagtgggac gcagtattac
     7321 tttactaccc cacagagaat gatccctggc gcccttatgt gttttggggt gttgggctaa
     7381 cgcggatcga tttcgccgat tttgtgggca ccagttacga aggtacctat tttgccatgc
     7441 cgatcgggct ggggttgaag ttccgtccca agtccgggcc catcatgcga tttgaatttg
     7501 tcgatcaaat cgtttttccc tcacgattca atacgaccca tcatttcgcc tttaccgtgg
     7561 gactggaaat gcggtttggg gcgcgccgca agatttactg gccgtgggaa tccacccgct
     7621 ggtaactcct cttgtctcac tgcgggcgcg acaaatcatc gcctgtggcg tgtgccggat
     7681 ctgaacttac ttggcgggaa actggtacgg ttttgtcacg agcaccatcg ccgtgggttt
     7741 ggcggaaaaa cttttcttgc ctgcccgata acgaagaagg atgg
//
LOCUS       DAOSUT010000182         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4757_length_7754_cov_10.167424, whole genome shotgun sequence.
ACCESSION   DAOSUT010000182 DAOSUT010000000
VERSION     DAOSUT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4757_length_7754_cov_10.167424"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PLD05_13565"
     CDS             complement(<1..1746)
                     /locus_tag="PLD05_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOP78516.1"
                     /translation="MDIFRHCCVGVCLAIITAVISVSRGEPADSSSVLPPGVKAVWDV
                     NKAFREVTATRERICINGLWQWQPAERGAETVPQDHWGYFKVPGAWPGITDYMQKDCQ
                     TVHAHPAWGNIRFGSLTAAWYRRTITIPASWANRRIALIAEYVNSLATVFLDGQKVGE
                     IVFPAGELDITSAVRPGETHTVSILVEALPLKGVMLVHRDSNTARQVQGSVNRRGLCG
                     DVWLVAQPQGVRIEAIRVETSIRKNLVRFVANLKNAEANKKYSLRAIVKEGTSVVKEF
                     VSPASLVGTTAESQLVLEASWRPEKLWDIHTPQNQYTVEVALLDERGQVLDAAWPERF
                     GFREFWIDGRDFYLNGTRIFLCAVPLDNAQIGAAWASYQGARESLQRLKSFGINFVYT
                     HNYDCQPGSHLSFSEILRAADDVGMLVALSQPHFAQYDWQAENAEETNGYRQHARFYV
                     QVAGNHPSVVMYSTSHNATGYNEDMNPDLIDGIHDPRDGWSLNNARRALRAEAILRQL
                     DPTRIVYHHSSGNLGSMHTSNFYPNWVPIQEMCDWFEHWATVGVKPFFTCEYGAPFTW
                     DWAMYRGWYKGQREFG"
     gene            complement(1815..5336)
                     /locus_tag="PLD05_13570"
     CDS             complement(1815..5336)
                     /locus_tag="PLD05_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOP78517.1"
                     /translation="MRCTTMLYRTAVLTLTAITACIAIPFPALAQPWNCHVIDGTISG
                     ADGVRIADANGDGLMDVVTGFEQAGQTRVYINPGPKRVHQPWPFVTVGSTPDVEDAVF
                     ADIDSDGRPDVVSCCEGRTRRVFVHWAPGPEQDYLNAKLWQTVELPLPEHDTMWMYCL
                     PLDLDGQHGLNLVLGGKQTDSGKGAALGIAVTPAEPRNVTEWRWIELTRVGWIMSIEA
                     LDIDRDGDVDLIYSDRYSVKENGGEPSGVYWLENPGSEKIGDPWKKHPVGAIGRQVMF
                     IDVGDFDSTGRLQVFAAVKPNEIHHLVAPTDPRQPWQVKEVIKVTGADFEVGTSKAVR
                     ARDLNGDGRFDLAFTCEGATGQRRGVVWLEQTEKGWQVHDLSGPVGIKFDRIEFVDLD
                     GDGDFDLLTTEESEATSTPGVAGLGVVWYENPAKGSSAAARPQPALPEGNAPQPVSFP
                     YFGHPSHAVVWRNWGLISAEDLARVLNTTPENIRNLAQAMGLPAHPALAPDFRRRGYI
                     TLVRRNWHLLPYDQLLDLLGMTAEELAYSLREDDFLFVKLGNHKPRCERVVFQAPDET
                     VAKQIGWIRQVVREHFGAQLAVPEEPRFAFVEELSRPRRAVPPQPSTSTQEAPRYLYS
                     YFGPYGDPLSDPRIDPYPDGLLERLAELGVNGVWLHVVLRNLAPGEPEFPEFGAGCEV
                     RLENLRKLVERAKRYGIGIYLYINEPRAMPWEFFRNHPEIAGVAEGQYVTMCTSHPKV
                     RAWLTRSLQYVFSQVPNLAGVFTITASENLTNCASHGRHKDCPRCRDRSPDEIIAEVN
                     AAIEAGVHAASPRAHVLVWDWGWHGHGDASSLIPLLPKSVWLMSVSEWAQPFERGGVA
                     GRVGEYSLSVVGPGPRALKHWKVAQENGLKTAAKLQLGATWELSAVPFVPVLDLVAEH
                     CSRLAQVGVDGCMLSWTVGCYPSPNLAVANRLLSDKSATPDAVLNTVAQQRYGPRAVG
                     AARQAWRLFSTAFQEYPFHIQVVYRGPQHWGPANLLFAEQTGYRATMVGFPYDDLDGW
                     RGPYPREVFAQQFSRVATIWADGLREFAKVVALADEDKKPIAEADYRVAQAAQIHLAS
                     VANQARFIMARNRYYSARDAADRAQAAATLDEILTAEISLAKQLYSITKCDSRIGFEA
                     SNHYYYVPLDLVEKVINCEYLRQQFRQ"
     gene            5848..7701
                     /locus_tag="PLD05_13575"
     CDS             5848..7701
                     /locus_tag="PLD05_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78518.1"
                     /translation="MSLYPSAVARIVPLGRSGLTTLGILLGLLYMMMTHVAVARAENN
                     PNSRVIRHSDVVFMYDNPDLYEAYGCTVLGWAGARSPERIALAHQRGVRLFAVSVGFR
                     TEFARMIDFTPDFLQAACRNYAGDTFIVPWLWDHKHKGQPAWWFCTNHPLYRQYLMKR
                     LEETVASGADALHIDDYTGTASAVTWLDACFCSACMAGFRTYLKTKLSAERQKELGIT
                     DLDTFDYRQFLMNRGITPERYRKERPGLPLATEFLDFQVQAATDFVAEYRRKGEELKG
                     GPLPLAVNSGLTSPLSLAITPHVSYFCCEVHHAAESRKVPFHPIYVYKLADGLNRPVT
                     STASGGDWAYIMENNLPGLVRTWVALSYAFGHNLMAPHRQWCYTQKKGTHWYSGPTEE
                     YAPLYRFVREHASILDDFEAVGRIAVLYDNHSRRLGKGDIERITMSLADANWPFKVLV
                     QGNDWLPGYRVDPTTLGQFDKVIVPEDLQPLPEIQAMLDQARSQGKLVTWSAGDNLAL
                     LGKNPVQVSSPGAVFIVPRVKTTGEHPVMAIHLVNRQYDGEKDKMIPLTNLTLTIDET
                     VLPGRADGCRSAKLHSPGKEDMTVVVKPEAKHLTVTIPEIDLWTIVELEWR"
BASE COUNT         1756 a         2298 c         2050 g         1650 t
ORIGIN      
        1 gccaaactcc cgctgtccct tgtaccaacc ccgatacatc gcccaatccc aggtgaaggg
       61 cgctccatat tcacatgtaa aaaacggctt gacccccacc gtggcccagt gctcgaacca
      121 atcacacatc tcttgaatag gtacccagtt ggggtaaaag ttgctggtat gcatgcttcc
      181 caagttaccc gacgagtggt ggtaaacaat tcgcgtggga tcgagttgcc gcagaatggc
      241 ctctgcccgc aatgcacgcc gggcattgtt cagcgaccaa ccatcccggg ggtcatggat
      301 gccatcaata agatcaggat tcatgtcttc gttataacca gtcgcattat ggctcgtcga
      361 atacatcacg accgagggat gattgccggc cacctgtaca taaaaccggg catgctggcg
      421 ataaccattc gtttcctcgg cgttctcggc ctgccagtca tattgggcga agtgcggctg
      481 agacaaggcc acaagcattc ccacatcgtc agccgcccga agaatctctg aaaaactcag
      541 atgtgaaccg ggctggcaat cataattgtg tgtgtacaca aaattgattc cgaagctttt
      601 cagccgctgc aaactttcgc gggccccttg ataagaggcc caggccgcgc cgatttgggc
      661 gttatcgagc ggcacagcac agaggaaaat ccgcgtgcca ttgaggtaaa agtctctccc
      721 atcaatccag aactcgcgaa atccgaatcg ctccggccac gcggcgtcga gaacctggcc
      781 tcgctcatcc aatagcgcaa cctcgaccgt gtattgattt tgtggcgtat gaatatccca
      841 caacttctct gggcgccacg atgcttccag gacgagctgg ctttcagctg tggtgccaac
      901 aagcgaggcc ggggacacaa attccttaac aaccgaggtg ccctccttga caatggcccg
      961 cagtgaatac tttttgtttg cttctgcgtt cttcaaattt gcgacaaacc tcacgaggtt
     1021 tttacgaata gaggtttcaa cgcggattgc ctcgattcga accccttgcg gttgggcgac
     1081 gagccacaca tcgccacaca ggccacgacg attgaccgag ccttggactt gccgggccgt
     1141 gttgctatcg cgatgaacca acatcactcc cttgagcggt aatgcttcca caagaattga
     1201 cacagtgtgc gtctcccccg ggcgaacggc ggaagtgata tccagctcac cagcgggaaa
     1261 aacgatctct ccaacttttt gcccatcaag aaagaccgtc gccagtgaat tcacatattc
     1321 ggcaataagt gcgattctcc ggtttgccca cgaagccggg atcgtgatcg tccgccggta
     1381 ccatgctgcc gttaagctgc cgaaacggat attgccccag gcaggatgag catggacggt
     1441 ttggcaatcc ttctgcatgt agtcagtgat tcccggccat gccccgggga ccttgaaata
     1501 tccccaatga tcttgaggaa ccgtctccgc accgcgctca gccggctgcc actgccacag
     1561 gccgttaata cagatccgtt cgcgggttgc agtgacctcg cgaaaggcct tgtttacatc
     1621 ccagactgct ttcactcctg gaggaagaac agacgaagaa tctgctggtt ctccacggct
     1681 cacacttata acagccgtga taatggccaa gcaaacgcca acacagcaat gacggaaaat
     1741 gtccatgact cacctccccc aatttgaact ccgtacattg gagcggccag cgaaagcccg
     1801 cggcgcgatc aatattactg cctaaactgc tgccgcaagt attcgcaatt gatgaccttt
     1861 tccactaggt ccaacgggac gtaataataa tggtttgacg cctcgaaacc aatccgcgag
     1921 tcacatttgg taatgctgta cagttgtttg gccagcgaga tttcggccgt aaggatttcg
     1981 tcgagtgttg ccgctgcctg cgcgcgatct gccgcgtctc gggccgagta ataccgattg
     2041 cgggccatga taaaccgcgc ctgattggcc acgctagcca ggtgaatttg ggccgcttga
     2101 gctacccggt aatccgcttc ggctataggc ttcttatcct catcggccag ggcgacaacc
     2161 ttggcaaatt ccctcaatcc atcagcccag atggtagcaa cccgactgaa ctgctgggcg
     2221 aacacctcgc ggggataagg tccccgccaa ccatccaggt cgtcatacgg aaatcctacc
     2281 atcgtagccc gatagccggt ttgctccgcg aacaataggt tggccgggcc ccagtgctgg
     2341 ggaccgcggt aaaccacttg tatgtgaaat ggatattcct ggaaagccgt actaaagagc
     2401 cgccacgcct gccgggctgc cccaactgcg cgcggcccat agcgctgttg ggcgactgta
     2461 ttcaagacgg catctggggt tgcactcttg tcagagagca ggcggttcgc caccgccaga
     2521 ttaggcgaag ggtagcagcc caccgtccaa ctcaacatgc aaccgtccac acccacctgg
     2581 gctagccgcg agcaatgttc ggctaccaaa tcgagcaccg gcacgaaggg aacagcagac
     2641 aactcccaag tagctcccaa ctgcaacttg gcggcggttt tcagaccgtt ttcctgggcc
     2701 accttccaat gtttcagggc gcgaggcccc ggccccacaa cggacagcga atactccccg
     2761 acccgaccgg caacgccacc ccgttcaaaa ggctgtgccc attcgctgac cgacatcagc
     2821 caaaccgact tgggtaaaag gggaatgagg ctcgaggcgt ccccatggcc gtgccagccc
     2881 caatcccata ccaagacatg ggcccgcggc gaagcagcgt gcacaccggc ctcgatggcc
     2941 gcgttgacct cggcgatgat ctcatcaggg gaacgatccc gacatcgcgg gcaatctttg
     3001 tggcgtccat gcgatgcgca attggtgaga ttctccgagg ccgtgatcgt aaacacccct
     3061 gcaaggttgg ggacctgcga aaaaacatat tgcaaggagc gcgtcagcca agcacggact
     3121 ttgggatgac tggtacacat tgtcacgtac tggccctccg caacgccggc aatctcgggg
     3181 tgattacgga agaactccca gggcatcgca cgcggctcat tgatataaag atagatcccg
     3241 attccatacc tcttggcacg ttctactaac tttctaagat tctccagtcg cacttcacac
     3301 cccgccccga attcgggaaa ttctggttct cccggcgcga ggttccgcaa gaccacgtgt
     3361 aaccacacac cgttaacacc caattccgcc aagcgttcca gcaggccatc aggataggga
     3421 tcaatcctcg gatcgctcag cggatcaccg tagggcccaa agtacgagta aagatagcgg
     3481 ggggcttctt gcgtggatgt tgacggctga ggtggcaccg cccgtcgtgg acgacttaat
     3541 tcctcgacaa aggcaaaccg cggctcttcg ggaacggcaa gttgagcgcc gaagtgttcc
     3601 ctgaccactt ggcgaatcca accgatttgt tttgcaacgg tctcgtcggg agcttgaaac
     3661 accacacgct cacaacgcgg cttgtggttg cccaacttca caaacagaaa atcatcctcc
     3721 cgtagagaat aagccagttc ctcagcggtc attccgagga ggtcgagcaa ttgatcgtac
     3781 gggagtaaat gccaatttcg tcgaacgagt gtaatgtaac cacggcggcg gaaatccggc
     3841 gctagcgctg ggtgggcagg caggcccatc gcctgcgcca agttgcgaat gttctccggc
     3901 gtggtgttca gcacgcgagc aagatcctcc gccgaaatta aaccccaatt ccgccacacc
     3961 acggcatggg aggggtggcc gaagtaaggg aaagagactg gctgaggggc gttaccctcg
     4021 ggcaaggccg gttggggacg ggccgctgcc gatgaacctt tcgcgggatt ttcgtaccac
     4081 accacgccca accctgccac tccgggggtt gaggtcgctt cgctctcctc ggttgtaagg
     4141 aggtcaaaat ccccatcacc atcgagatcg acaaactcga tgcgatcaaa cttgatgccc
     4201 accgggccac tcagatcgtg cacttgccaa cctttttcgg tttgttccag ccacacgacc
     4261 cctcgccgtt ggccggttgc cccttcgcac gtgaacgcca gatcgaaccg gccatcaccg
     4321 ttcaggtcgc gcgctcgcac cgccttgctg gtgcctactt cgaaatccgc acctgtaact
     4381 ttgataacct ctttcacttg ccaaggttga cggggatcag tgggagccac aaggtggtga
     4441 atttcattcg gcttaaccgc cgcgaacacc tgaagtcggc cggtgctgtc aaaatctccc
     4501 acgtcgatga acattacctg gcgaccgatc gccccaacgg ggtgtttttt ccaaggatcc
     4561 ccgatctttt cggaacccgg attttccagc cagtacacgc ccgacggttc accgccattt
     4621 tccttgaccg aataccggtc cgaataaatg agatcgacat caccatcacg gtcaatgtcg
     4681 agggcttcaa tactcatgat ccagccgacc cgcgtcagct cgatccaacg ccactcagtc
     4741 acgttgcggg gctcagcggg cgtcacggca atccccaagg ccgcgccctt acctgagtcc
     4801 gtttgttttc cccccaagac gaggttcagg ccgtgctgac catcaaggtc aagcggcagg
     4861 caatacatcc acattgtgtc gtgctccggc aacggcaatt cgacggtctg ccagagtttt
     4921 gcattaagat aatcctgctc tggtcccggt gcccaatgga caaagactcg ccgcgtgcga
     4981 ccctcgcagc aggagacaac gtcaggccga ccgtcgctgt caatgtccgc aaagacggcg
     5041 tcctctacgt cgggtgtgga gccaaccgtt acgaacggcc acggttggtg cacacgcttc
     5101 ggcccgggat tgatgtacac ccgcgtttgg cctgcttgct cgaaacctgt caccacatcc
     5161 ataagtccat cgccattcgc atcggcaatg cgaaccccgt ccgcacctga aatggtgccg
     5221 tcaatgacat ggcagttcca cggctgcgcc aacgcaggaa atggtatggc gatgcatgcg
     5281 gtgatggccg tcaaggtcag aacagccgtt cgatacaaca tcgtggtgca gcgcataaca
     5341 gcctcctttg ggtggcgtcc tataatgcga gggaagccat cctgcatagt cacgcccgct
     5401 tgcaggatac aagcttcccc gcaggtctga gaacgatcgt gcgatggtcg ttcacagatg
     5461 ttctttctgt cacccacgcg gtttttcggt ggacgtcggc gattttgggc gccggggtcc
     5521 gccttgtcaa gcgctcgcct tgaagattgt ctgacgccat ggccagtcag cggtttcttt
     5581 ccacagaatg ccccagcaag gcgataagct tctccttagc gtgtcatcat cttgaaaaag
     5641 ctcgtgctac gccggtcgga caactgcgat atgctggccg ctcatcgtca cagaaagcta
     5701 cggacctggt aattctgatt ttgatgactt accatatccc ttggtaaacg acgggtaaga
     5761 ggaagaaatt cgtcaatgcc ttttgaaact ccacccctcc gacgagttga ttctccatca
     5821 atctccgctt tgaagaagga gtaccttatg agcctgtacc catcggctgt cgcaaggatt
     5881 gtcccacttg ggagatccgg actcacaaca ctcgggattc ttctcggcct gctctacatg
     5941 atgatgaccc atgtggccgt cgcacgggca gagaacaatc caaactcgcg ggtcatccgg
     6001 cattctgatg ttgtgttcat gtacgataat ccagaccttt atgaggctta cggctgcacc
     6061 gtgctaggtt gggcgggtgc ccgatcaccg gaaaggattg ccctagctca ccagcgaggt
     6121 gttcgactgt tcgccgtcag tgtcggattt cgcacggaat tcgcccgcat gatcgatttt
     6181 acgcccgact ttctgcaggc tgcctgtcgt aactatgccg gcgatacgtt cattgtgccg
     6241 tggctttggg atcacaaaca caaaggacaa cctgcatggt ggttttgcac gaatcatccc
     6301 ttgtatcggc agtacctcat gaaacggctt gaagagacag tcgcaagtgg ggccgatgca
     6361 ttgcacattg acgactatac aggcacggct tctgcggtaa cgtggcttga tgcctgtttc
     6421 tgttcggcct gcatggcagg atttcgcaca tatctaaaaa caaaactttc cgccgagcgg
     6481 cagaaagaac tgggaataac cgatctggac acctttgact accgccaatt tctcatgaat
     6541 cggggaatca ctcccgagcg ttatcgcaaa gagcgtcccg gtcttccctt ggctacggag
     6601 ttcctcgatt tccaggtgca ggcggcgacc gattttgtgg ccgagtaccg acggaaaggc
     6661 gaagaattga agggaggccc tctccccttg gcggtcaact ccggactgac aagcccgctc
     6721 agtctcgcca ttacgcccca cgtcagttat ttctgttgtg aagttcacca tgcggcggaa
     6781 tctcgtaagg taccgttcca tcccatttat gtttacaaac tggcggatgg tctcaatcgg
     6841 cctgtcacct cgaccgccag cggtggggat tgggcctaca tcatggagaa caatttgccc
     6901 ggcctcgtgc gaacttgggt agccctttct tacgccttcg gccacaattt gatggccccg
     6961 caccgacagt ggtgttacac gcagaagaaa ggaacacact ggtactccgg tcccaccgaa
     7021 gaatacgcac ctctttatcg ctttgtccga gaacacgcta gtatccttga tgattttgag
     7081 gccgtgggac gcattgccgt tctttacgac aatcattccc ggcggctggg aaaaggggac
     7141 attgagcgca tcacgatgag cttggccgac gcaaattggc cctttaaggt gctcgtccaa
     7201 gggaatgact ggcttcctgg ctatcgcgtt gacccaacga cccttggaca gttcgataag
     7261 gtcatcgtgc cggaagacct acaacctttg ccggagatcc aggctatgct agatcaggcc
     7321 cgatcccagg ggaaactcgt gacgtggtcg gcgggcgaca acctggcgct gctgggaaaa
     7381 aatcctgtac aagtttccag tcctggagcg gtgttcatcg tgccgcgcgt caagacgacg
     7441 ggtgaacatc cggtcatggc aatccacctc gtgaatcgcc aatatgatgg agagaaggac
     7501 aagatgatcc cgctgacgaa tctcaccctg accattgacg aaacagtgct ccccgggcgg
     7561 gcggatggat gccgctcggc aaagctccat tcaccgggca aagaggacat gaccgttgtg
     7621 gtcaaaccgg aggcgaaaca ccttaccgtg accatccccg agatcgacct ttggaccatt
     7681 gtcgagctcg agtggcgctg acaagattgg gagcgaaacg tataatcaaa aatggctggt
     7741 gagcgcggcc gtca
//
LOCUS       DAOSUT010000183         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4758_length_7754_cov_9.588388, whole genome shotgun sequence.
ACCESSION   DAOSUT010000183 DAOSUT010000000
VERSION     DAOSUT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_7754_cov_9.588388"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLD05_13580"
     CDS             <1..321
                     /locus_tag="PLD05_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOP78519.1"
                     /translation="WEGMRIEEIRTRQPKIYRQWQEIPDCICPPEGETIEEAETRVTE
                     CLQRLLRRHKNQAVGLVVAEPLASIVKRLITQTPLGDLWKAESRHGQIEILRWDPKPI
                     ETAS"
     gene            complement(458..1423)
                     /gene="pyrF"
                     /locus_tag="PLD05_13585"
     CDS             complement(458..1423)
                     /gene="pyrF"
                     /locus_tag="PLD05_13585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOP78520.1"
                     /translation="MMPDNQRPQRFIDRLESAVRRRRTPVLVGIDPHLELLPPNVGAG
                     DVENRWMLPREKLAEAVRVFCAGVLDVVASLVPAVKFQLAFFEQLGPEGMRVLLELVA
                     RARREGLLVILDGKRCDIGSTAAAYAQGMLGPPSVSPWGGDAITVSPYLGEDSLQPFV
                     ETAVARQAGIFVLVKTSNPGGGMFQDLVADGMPIYRHVARWVEHQAVTTKGQCGYGDV
                     GGVVGATYREQLVELREAMPHTWFLVPGFGAQGATPRDVAAAFDAAGLGAIVNNSRGI
                     LFAYRRREFERFGPARWQEAVEAATRDMIEQLRAETPAGRLVGGL"
     gene            complement(1686..4070)
                     /gene="pheT"
                     /locus_tag="PLD05_13590"
     CDS             complement(1686..4070)
                     /gene="pheT"
                     /locus_tag="PLD05_13590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOP78521.1"
                     /translation="MKISLDWISDYVDINDIPVDVVADRLTMCTAEVEDVLEVRRSVA
                     QVVVGEVIDCRDIPAAPNLKLVTVDCGTRRFQTVCGAPNVRKGLKAPFAPPGTRLANG
                     VEIAVAEVSGHRSEGVLCSPAELGMSRWHEGLLECPPSLPNGAPLAQWIPDRDVLIEI
                     DNKSLTHRPDLWGHYGFARELAAIFRRPLRPLPVVDLSQYDHLPTYPLTVDDYENCPC
                     YGCIDLRVRAGVPAPLVIQRRLHALGQRTFNLMVDLTNYIMWEIGQPTHAFDGDRVHA
                     IRVAPLGKPGTFVTLDGQERQLLPDDLLIWNEKEPVALAGVMGGLESEVTPTTTHVLL
                     ESANFKASRIRRTSVRLDLRTEAAQRFEKGQPPVNVKIGTARALQCLIDSGEPFEVLS
                     RFTVAGDLKEAPRPLSIPWRTVDTMAGQKIPRQEAVGILRHLGFLAEEKGETLEVGIP
                     AHRSEKDISIPADVVEEILRIYGYDRIEPRLPEGPMAALPVNVGLRKEHQARRVLAGA
                     HGFVEVQNYVWLDDNWLAELGYQPGETLTLKNPPAQTCRRLRPVLLPNLLALVRPNRA
                     HRDMFRIFEIGRVFRPQANDGCEERTHLAGVSFRSSGKGDQEEHFRSIRGALEDLGVV
                     LTGQALRFVAGQDSPFPWCVPGYWVKILLGDALVGAAGVLDPVLARVVALEGQVVWFE
                     LDLEAIQGPIYPVVHYRPLAVYPRSWQDFSLLWDVTKGFDSLEAVLSKFQHPLISERE
                     FLYVFKGKGLPQGQASYTFRYWLAAPDHTLTGEEIEGFRTAFLSFLQENGISLR"
     gene            complement(4174..5199)
                     /gene="pheS"
                     /locus_tag="PLD05_13595"
     CDS             complement(4174..5199)
                     /gene="pheS"
                     /locus_tag="PLD05_13595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOP78522.1"
                     /translation="MDEILEQLRRDVAAATDSKQVEQVRVKYLGRKGIITTLAKNTDF
                     SRLSSEEKRSFGQRLNALKSEAEQLLKEALEKAKAAEKDLVRKVAGLDLSLPGIDRRL
                     GSIHPIALVQMELEEIFQGMGFRVLTGYEVELEYYNFDALNIPPDHPARDMQDTFWLD
                     NGMLLRTHTSANQVRALETYGVPLRAIFPGRCFRNEAIDASHENTFYQLEGLLVDRDI
                     SVAHLIAVMKTLLSQVFKREVTVRLRPGFFPFVEPGFELDVQCLICGGKGCATCKQTG
                     WIELIPCGLVHPKVLEFGRVDPNIYSGFAFGMGLTRMAMMKFGIPDIRLFNSGDIRFY
                     EQFPAAF"
     gene            5748..6911
                     /locus_tag="PLD05_13600"
     CDS             5748..6911
                     /locus_tag="PLD05_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP78523.1"
                     /translation="MEADLSVIITTFERPWHLRRVLLSLAIQRDCHCRLEVVVADDGS
                     RDETPQVVEEFARQVDFPVRFVTHPHEGFWPARARNEGFIASTGRYLLFLDGDCVIPP
                     DHLAAHMRMRRPHRAWTGDCIRLDQMMTQRMTEDAVKNADYLRWPLRAEYRRLAIAHW
                     KAVFYHAIRHPRKPRVLSGNLAVWRHDFLRINGFDEQYRGWGCEDDDLGLRLHQAGIR
                     VFSIRRLTRTYHLWHPRVESAPSKVSNGLNFAYLHRAGRLSCCRNGLKKRSWKDLHVV
                     WTGNPAAISDYCGAELCPPDGCPSSPFGGRTSDRPEVEILIWDWSARSFRTDAECRVL
                     VIIKGQTPPKGVVRASDWVLADGERLDTFRPWIKQGARLAPLERFREALEEIG"
     gene            complement(7389..>7754)
                     /locus_tag="PLD05_13605"
     CDS             complement(7389..>7754)
                     /locus_tag="PLD05_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOP78524.1"
                     /translation="RRFREDPQHRILLMSYGAGSVGLNLQFVNYVFLFDRWWNPAVED
                     QAINRAHRIGAAGPVIVTRFLTVDTIEQRINMVLEQKRQLFETIFSGTVEPKKLALTQ
                     EEIFGLFDLKTMATRAKAA"
BASE COUNT         1706 a         2310 c         2153 g         1585 t
ORIGIN      
        1 tgggaaggga tgcggattga ggaaatccgc acacggcaac ccaagattta ccgtcagtgg
       61 caagaaatcc ctgattgtat ctgcccgcct gaaggggaga cgatcgagga agccgaaacg
      121 cgggttacgg aatgcctgca acgcctcttg cgtcgccaca agaatcaggc ggtgggattg
      181 gttgtggcag agccgctggc aagcatcgtt aagcgactga ttacccagac tcccctaggt
      241 gatctctgga aggcggagtc ccggcacggc cagatcgaaa tcctccgctg ggaccccaag
      301 ccaatcgaaa cagcctcgta accggccaga gaaagtatcg gggccttctt aacgatccgt
      361 caaaagcagg agcaggctac cccgttgccc ggtctggacg tttggggtca cggcccaata
      421 gcacctattc catcgggtta gcggctgtga tcggctgtca caagccaccc accaaccgac
      481 cggccggtgt ttctgcccgc agttgttcga tcatatcccg ggtggcggcc tcgacggcct
      541 cttgccatcg cgcgggccca aagcgttcaa attctcgccg ccgataggca aataggatgc
      601 cacgggaatt attaacgatc gcccctaaac cggccgcatc aaaggctgcg gccacgtcgc
      661 ggggtgttgc accttgtgcc ccaaatcctg gcacgagaaa ccatgtgtgg ggcatggctt
      721 cgcggagctc gaccagttgt tctcggtagg tggcacccac aacgccgccg acatcgccgt
      781 agccgcattg ccccttggtc gtaacggcct ggtgttccac ccaacgggct acgtggcgat
      841 aaatgggcat cccgtcagcc acaaggtctt gaaacattcc cccgccggga ttagaggtct
      901 tgacgaggac aaagatccca gcctggcgcg ctacggccgt ttccacaaat ggttgaagac
      961 tgtcctcccc caagtatggg ctaacggtga tggcatcgcc gccccaggga ctgacggatg
     1021 gggggcccaa catcccctgg gcgtacgcgg ccgccgtact cccgatatcg cagcgtttgc
     1081 cgtcaagaat gacgagaagt ccttcccggc gcgctcgagc taccagttcc aaaaggaccc
     1141 tcatcccctc cggacctaac tgctcaaaaa atgccaattg gaacttcacg gcggggacca
     1201 gcgaggccac cacgtcgagg acccccgcac aaaagaccct gacggcctcg gcaagtttct
     1261 cgcgaggaag catccaacgg ttctcaacat cgcccgcacc cacgttcggt ggaagaagtt
     1321 ccagatgggg gtcgatcccc accagaaccg gcgtccgacg ccgccgaacg gctgactcaa
     1381 ggcgatcgat gaatcgctga ggtctttgat tgtcaggcat catggctcct tccagcgagc
     1441 tttgtgtgtc aaactcgtta ttcttcgggc aatatcgcag caagttgcct ggtcaacgtg
     1501 ggagggttta tcccattcta ttgacccgcg ccacggcgga acacatctgc ggatacctcc
     1561 gacgacacga agggggcaaa acccccgggg gttgtccgta gtcgtcttga cttggcattc
     1621 atccatcgcc ccaattgccc catttagcaa gattcgggcc gcaaatcgcc acggtgaaat
     1681 gggggttagc ggagcgaaat cccgttttct tggaggaagc tcaaaaaggc ggtccgaaac
     1741 ccttcgatct cttcccctgt caacgtatgg tcgggggcag ccaaccagta gcggaacgta
     1801 taacttgctt gaccttgagg gagtcccttc cctttgaaga cataaaggaa ttcccgctcg
     1861 ctgataagcg gatgctggaa tttggaaagg accgcctcca gcgaatcgaa gcccttcgtc
     1921 acgtcccaca acagggaaaa atcctgccac gaacggggat aaacggccaa cggtcggtag
     1981 tgcaccacgg gatagatcgg gccttggatc gcttctagat ccagctcaaa ccacacgacc
     2041 tggccttcca aagcgaccac cctggcaagg accgggtcga gaacgccggc tgcccccaca
     2101 agtgcgtccc ccaagagaat cttcacccag tagcccggca cacaccacgg aaatggcgag
     2161 tcctgccccg cgacgaaacg gagagcctgc ccggtcagca ccacgccgag gtcttccagt
     2221 gccccgcgga tgctgcggaa atgctcttct tgatcaccct ttcccgatga acgaaaactc
     2281 accccggcca ggtgggtccg ctcttcacat ccatcattgg cctggggccg aaatacccga
     2341 ccaatctcaa agatgcgaaa catatcccga tgggcccggt tgggtcgaac cagggccaat
     2401 agattgggca gcaacacagg gcggagtcgg cgacacgtct gggcaggagg attcttaagg
     2461 gtcagtgttt ctcccggctg ataacccaat tcagccagcc aattgtcatc gagccagaca
     2521 tagttctgga cctcgacaaa cccatgggca cctgcaagca cgcgccgggc ttgatgctct
     2581 ttccgcagtc ccacgttgac aggcagggcg gccatggggc cttcgggaag acgtggttcg
     2641 attcgatcgt agccataaat gcgaaggatt tcctccacca cgtcggccgg tattgaaatg
     2701 tccttctcac tccgatgggc aggaatccca acctccaggg tctcgccctt ttcctcggct
     2761 aaaaaaccca aatggcggag gatgcccacg gcctcttgcc gaggaatctt ttggccggcc
     2821 atggtatcga ccgtccgcca ggggatggac aaaggacgcg gtgcctcttt caagtccccc
     2881 gccaccgtaa accgggaaag gacctcgaac ggttctccgg aatcgatcaa gcactggagg
     2941 gcccgggccg tgccgatctt cacgttcacg ggcggttgtc ccttttcgaa tcgttgggcg
     3001 gcctccgttc gcaaatcgag acgaaccgag gtccgacgga tgcgggaggc cttaaagttt
     3061 gcactctcca gaaggacatg tgttgtggtg ggagtgacct cactttccag cccgcccatc
     3121 acgcctgcca aggccaccgg ctctttctca ttccaaatga gtaggtcatc gggcaggagt
     3181 tgccgctctt ggccgtccag cgtcacgaac gtccccggct ttccgagggg tgccacacgg
     3241 atggcgtgaa cacgatcgcc atcaaaggcg tgggtgggct gtccgatttc ccacatgatg
     3301 taattggtca gatcgaccat caggttgaag gtccgctgtc ccagggcatg gagtcgtcgc
     3361 tggatcacaa ggggggccgg cacacctgcc cgaacccgca agtcaatgca accataacag
     3421 gggcagttct catagtcatc caccgtcaat ggatacgtcg gcaaatggtc gtactgcgaa
     3481 agatcgacga ccggtaacgg ccgcaatggc cgacggaaga tcgcggccag ttcgcgggca
     3541 aaaccgtagt gcccccagag gtccggccgg tgggtcaggc tcttgttatc aatttcaatc
     3601 aagacgtccc gatcgggaat ccattgggcc aacggtgcgc cattcggtag cgacggtgga
     3661 cattccaaaa ggccctcgtg ccagcggctc atccccagtt ctgccggtga acagagaacc
     3721 ccttcgctac ggtgaccgct gacctcggcc acggcgattt ccacaccatt ggcaagccgg
     3781 gtacccggcg gggcaaatgg tgccttcaag cccttgcgaa cattcggcgc tccacaaacc
     3841 gtttggaaac ggcgcgtgcc acaatcgacg gtgacaagtt tcagattcgg cgccgccggg
     3901 atatctcgac aatcaataac ttcgccaaca acgacctgag ccacgctccg ccggacctca
     3961 agaacgtctt cgacctccgc cgtacacatg gtcagtcgat cggccactac atccacgggg
     4021 atgtcgttga tatcgacgta gtcgctaatc caatcgagag agatcttcac agctcactcc
     4081 gtgacaccac ttcactaatc gttttactgt cagtgattca aaaggcgttc gttttatctc
     4141 gtgtgcgccc cacacacagc cagaaccccg gcttcagaat gctgccggga attgctcgta
     4201 aaagcggatg tcgcccgagt taaataagcg aatgtcgggg atgccgaatt tcatcatggc
     4261 catacgggtc agccccatcc cgaaagcaaa gccactatag atgttgggat cgactcgccc
     4321 aaactcgagc actttgggat ggaccaggcc gcaggggatc aattcgatcc acccggtctg
     4381 tttgcaggtg gcacatccct taccgccgca aatgaggcat tgaacgtcca attcgaagcc
     4441 cggctcgaca aacgggaaaa agccgggacg aagccgcact gtcacttctc gcttaaaaac
     4501 ctggcttaac aatgtcttca ttacggcgat gaggtgagcc accgaaatat cacggtccac
     4561 caggagacct tccaactggt aaaaggtatt ctcgtgactg gcgtcgatcg cctcattgcg
     4621 gaagcagcgt cctgggaaaa tcgcccgcaa cgggacgccg tacgtctcta acgcccgcac
     4681 ctggttggcc gaggtgtgcg tccgcaagag catcccgtta tcgagccaga acgtatcctg
     4741 catgtcccga gccgggtgat ctggcggtat attgagggca tcgaagttgt agtactccaa
     4801 ctccacttca tagccggtca acacgcggaa gcccatgccc tggaaaatct cttccagttc
     4861 catctgaacc agggcaatgg ggtgaatact tcccagccgg cgatcaatac cgggaaggga
     4921 aagatccaag cctgcgacct tgcggacgag gtccttttct gcggccttgg ccttttccag
     4981 ggcctctttt agcagttgct ccgcctcgct tttgagggcg ttcaatctct gcccaaagga
     5041 ccgcttttct tctgatgaaa gtcggctgaa gtctgtgttc ttggccaagg tggtgatgat
     5101 tccctttcgg ccgagatact tgacccggac ctgttccact tgctttgagt ctgtggctgc
     5161 tgccacatca cggcgaagtt gctcaaggat ttcgtccata ccagtaagtc aatcgcttaa
     5221 attccaaagt tagatgcgtt aggttaaaac gcggtgttga tatctcgcta atcggcccgc
     5281 aattcgctgc cggtgcccgc acaatgaggt acgctgaccg acgaatgatc aagaaatgcc
     5341 cagtttatcg cgcgacgtca atgttgggta tctgggtttg gtcgcaaaag tggcccactt
     5401 tccctcacaa cgttggcaga agagcaacat ccgttgtcca aaatgcgcat cactcatcat
     5461 aatagcgggt ggcaaagggt gataagagcc tatcccataa tctcccctcg cccgttcggc
     5521 gggagagggg cagggggtta tggtggctcg aatcgctgcc ggtgttctcg atcgactccg
     5581 acggtcgctt gccctcaccc caaccctctc ccagagggag agggagactt tttgggatca
     5641 attctaagtg cattttgagt aatctcggac cgtcgtgagg tgtcgctttc aaaggaatca
     5701 cctttttgcc gccgtcacga aaaccttgac aggaaagcgg acgtggaatg gaagccgacc
     5761 tatccgtgat catcacgacg tttgagcggc cttggcattt gcgtcgtgtc ttactgtcgc
     5821 tggccattca gcgggactgt cattgccgtc tggaagtggt cgttgcggac gacggatccc
     5881 gggacgaaac acctcaagtt gtcgaggaat ttgcacgcca ggttgatttt cccgtccgtt
     5941 ttgtcacaca tccccacgag gggttttggc cggcccgagc ccggaatgaa ggcttcatcg
     6001 ctagcacggg gcggtatctc ctttttctcg atggcgactg tgtcatcccc ccagatcatc
     6061 tagccgctca tatgcggatg cggcgccctc atcgggcatg gacgggcgac tgtattcgtc
     6121 tggaccaaat gatgacccaa cggatgaccg aagacgccgt gaaaaacgca gattatctcc
     6181 gctggccgct ccgggcagaa taccgacggc tggcgattgc ccattggaag gccgtcttct
     6241 accacgcgat tcgccatccg cgaaagcccc gggtcctgtc gggcaatttg gccgtgtggc
     6301 ggcacgattt cctgcggatc aacggtttcg atgaacaata ccgggggtgg ggttgtgaag
     6361 atgatgatct cggcttacgg ctccaccagg ccggcatccg tgtgttttcc atccgccgac
     6421 tcacaaggac ttaccacctt tggcaccccc gagtggagtc ggccccgtca aaagttagca
     6481 atggtctcaa ctttgcctat cttcaccggg cggggagact atcatgttgc cggaatggcc
     6541 taaaaaagcg gtcgtggaag gacctccatg tggtctggac gggcaatcca gccgcaatct
     6601 cggactattg tggggcggag ctatgcccgc cagacggatg cccttcatcc ccatttggcg
     6661 gacggacttc agaccgtccc gaggtcgaga tcctgatttg ggactggtct gcgagaagct
     6721 tccgcactga tgccgaatgt cgggttcttg tcataatcaa aggccagacc ccgcccaagg
     6781 gtgtcgttcg tgcctccgat tgggtgttag ccgatgggga aaggctcgac acttttcgcc
     6841 cgtggatcaa acagggggca cgcttggcac cgctcgagcg gttccgtgaa gccctcgagg
     6901 agatcggtta aacccggccg agggcactat cggaagctgg cctcggttcg atctggcgca
     6961 gtgtatcctc gacactggcg atcaaccccg tcaacgcatc agaaggcccc aagaaagcga
     7021 aagagcggat acgccgcacc gaaaagaggc gacatatcca ctctttcaaa gggaagcggg
     7081 ggttgtgctt cgcgcaaggt gattcgctct taacacccac gcgacggccg gcccaggtcc
     7141 gattcgcctc ccctgatcta ccataaaccg aaaaccgact ttgccggccg tcacacgggc
     7201 gagcaaacac tctcgttttt tgagcacgtt atgtgctctc aaaaaacggt attgccgatc
     7261 gctcgcccag cgcacgcaaa aaattttttg aaaaagtgcc tgccaccgtg gaatggcatg
     7321 cagacccgac gagagcccac cgcccgccgt cctttggaat gccattcccc acaaacgagt
     7381 aggttccttc aagcggcctt ggctcgggtg gccatcgttt tgaggtcaaa caggccaaag
     7441 atttcttcct gcgtgagtgc gagtttctta ggctccacgg tcccagagaa aatcgtctca
     7501 aaaagctgtc gcttttgttc caagaccatg ttgatccgct gttcgatggt atccacggtg
     7561 agaaatcggg taacgatcac cggtccggcg gccccgatgc ggtgtgcccg attgatggct
     7621 tgatcctcca ccgcggggtt ccaccaacgg tcaaaaagaa acacgtaatt cacgaactgc
     7681 aagttgagac ccacactccc tgctccgtaa ctcatgagca ggatgcgatg ttgtgggtct
     7741 tcgcggaagc gacg
//
LOCUS       DAOSUT010000184         7743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4765_length_7743_cov_9.622008, whole genome shotgun sequence.
ACCESSION   DAOSUT010000184 DAOSUT010000000
VERSION     DAOSUT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_7743_cov_9.622008"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1125
                     /locus_tag="PLD05_13610"
     CDS             388..1125
                     /locus_tag="PLD05_13610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOP78525.1"
                     /translation="MATVPAASTRGNYWEESRAPLASLIFVTPLLLVYEAGVLLMGPE
                     AVRNGAEVWLRHLLMNLGLGQYFFLPVLTVGILLAAHHVSRHRWLVRSSTLWGMFVES
                     LFLAGCLRVLLGLQSLLFQHVSRYVLATGGSGVDFTLAERIVAFVGAGVYEELVFRLI
                     LMNLVIIVLQFLRLAPPVTLWGGVLLSSFVFALAHHIGPYGEPLQLFNFLFRFVAGIF
                     FALLFVYRGFGIAAGSHAAYDLMVGLI"
     gene            complement(1398..7643)
                     /locus_tag="PLD05_13615"
     CDS             complement(1398..7643)
                     /locus_tag="PLD05_13615"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOP78526.1"
                     /translation="MSQVRCNWASAKTWTWVAVFGLCLGWIGSHYERVAWLLSVTPSL
                     AEIVKGATMDDAQRGQAANRLFKEGQFREALRLFEESLADPKADPFRVCNDLSLAWQC
                     LARLNQVSRIDGLIEKSVDVHAQNWRFLLTAAKLYLEIPHVGMIIGNEFKRGSDGDQG
                     KPVSCEERDRVRAMQLLVQAMPLVEKEPNRHEAAQFYLDFANIILFDRGWSEAWRLQA
                     LTDLSQLPDYEEGWYFHRGTRGAPVDEEGNPIFYAAPRTFDQAKNDGERWRWCLIQAK
                     ELNAALTDEVDYMFAEFLWTQFGVQTLADYWWYFGRLETEERQESETGTWALHTLGED
                     ETIARLAIGIKRFKLPDEFNFIRIWKTIAEKDRSPRKLDALKQLAQIFENRRQYPKAA
                     EYWKKVIPLLQGEERTWAEARLNQIVGNWGRFEPISTQPAGRGATVEYRFRNGTEVRL
                     TAYAIKLDQLLSDVKDYIRTQPLELNWEKINIENIGYRLIEKAETRYLGPVVATWTVP
                     LEPRPNHFDKRVTIATPLQKAGAYLLKAEMKNGNTCFVIIWVADAVIAQKPLDNGTWF
                     YVADAVTGQPIAKANVEFFGWQQVWRRPDAPRPQIEIKNFAEYTDAEGQIILGPKIQP
                     TEYQWLIIARAADGRMAFLGFTHLWFGRYYDAQYNATKVFTITDRPVYRPGHKVHYKL
                     WVREAKYDQPDTSAYANQLCVLELHSPKGDRLWTKPVTTDEYGGIEGEWEIPEDATLG
                     VYQLAVKGSRFRGGGSFRVEEYKKPEFEVTVQAPEKPIALGEKIEATIQAKYYFGSPV
                     THAKVKYKIERTGYTESWYPPRPFDWLYGPGYWWFAYEYTWYPGWHIWGCVRPRPFWI
                     DWSPEPPELIGEQEVAIGEDGTVKVTIDTAPAKMLHPDQDHRYKITVEVTDQSRRTIV
                     GSGEVLVTRQPFKVYLWLPRGYYRTGDVIEVHSCARTPDGRPVKGQGKLSLFRITYKD
                     AKPVETEARSWDLPTDEQGQARLQIKAAEPGQFRLVHTVTDEQGHTIEGGYVFTIIGE
                     SLRATEDFRFNDLELIPDKPEYQPGEKVQLQVNANCRDAKVLLFVRPANGVYQRPKLL
                     ALDGKSTIVPIEVTAKDMPNFFVEAVTVYDGKLYSEVKEIIVPPEKRVLNVEVTPSKT
                     EYLPGEKASVKIKLTDLAGQPFVGSTVVAIYDKSVEYISGGTNVPEIKAFFWKWRRHH
                     HSQTMHSLSRSSGNIPPRNQPSMEDLGIFGATVVEEVPEVLQSAAFGGGGRGIVRRSA
                     VMMGAPMEAAPRAAMAEGMAMEKAAAPAMNAAEAGADRAGPQEAEALVAPTVRKEFAD
                     TALWVGALTTKPDGTAEVELAMPENLTTWRIKVWAMGHGTKVGEAASDVVTRKNLIIR
                     LQAPRFFVQTDEVVLSANVHNYLKQGKSVQVRLELEGNTLEPLDELVRTINIAANDEA
                     RVDWRVRVKAEGEAVVRMAALTDEESDAMEMRFPAYVHGMDKMVPYSGWIRPDQEAVQ
                     FKIFIPAERRPESARLEVRFSPTLAGAMVDALPYLIDYPYGCTEQTLNRFLPAVITQK
                     VLIDLGLDLEDIRNKRINLNAQEIGAPEDREKQWQRYRREPVFSKVELDRIVKVGVQR
                     LTEMQLSDGGWGWFSGWGEFSSPHLTAIVVHGLQLAQENDVAIVPDVLQRGVAWLERY
                     QEEQLQLLRNADRKIKNVPWKSSADNLDAFVYMVLVDAGVRNEEMKNYLYRDRLNLSV
                     YALAMYGLALHKQQDQEKLAMVLRNINQYVEKDDENQTAWLNLGRWSWWYWYGNDIEA
                     MAYYLKLLARTDPRGELAPRLVKYLLNNRKHATYWNSTRDTALVIEAFADYIRASGEA
                     KPDMTVEVWIDGELKKAVEITPQNLFAFDNTFVLAGEALTTGEHVVELRRKGQGPLYA
                     NAYVSYFTLEDFIPAAGLEIKIDRRYYRLKPVQATTQVAGALGQVIDQRVAKYQREPL
                     QNLAELKSGDLVEIELILESKNDYEYLVFEDMKPAGFEPIDLRSGYTRDAAGAYVEFR
                     DNRVVFFCRALPRGRQSISYRMRAEIPGKFSALPTKGYGMYAPELRANSDEIKFRVVD
                     VPIVPVQ"
BASE COUNT         1518 a         2217 c         2153 g         1855 t
ORIGIN      
        1 caccgtgaca ttttctacat cgcacgcgtt taagcatgcg ccaaatgaga tgaagtgaca
       61 cgaccacaaa ccgaaagtac ctcgaaccta aagcggcctc gactctcgtt gattgtacga
      121 acggcggacc gtccccggca aggatttatg tcgattgagc ggtttacgcc aattgtaaca
      181 acaggacgcg ataatacgga cgacgctcgg aagagttgtc cgtgaacgtt gctcacccct
      241 tttgtcgaag tcaacataat aagactcacg ggaagccgcc gggagtgtca gcattttcgc
      301 ggcccgcata tctttcggac cttgagcgtt cgtacaaacg agtggcgatc aagtcggcct
      361 attagggcag tcacatattt tcaaactatg gcgaccgtac ctgctgcatc gacgcgaggc
      421 aactactggg aggagtcacg ggcccccctg gcgagtttga tatttgtcac accgctgctc
      481 ctggtttacg aggccggtgt cctcctcatg ggaccggagg ccgtgcgcaa tggggccgag
      541 gtctggctcc gccacctgct catgaatctt ggcttggggc agtacttttt tttgccggtc
      601 ttgactgtgg ggattctctt ggctgcccac cacgtttccc gccaccgctg gttagtccgc
      661 tcctcaaccc tctgggggat gttcgtggaa tcgttgtttt tggctggatg cttgagggtc
      721 cttctgggcc tacaaagcct gctgtttcaa catgtttctc gatatgtcct tgcaacgggc
      781 ggaagcggcg tcgatttcac tcttgccgag cgaatcgttg cctttgtcgg ggccggtgtt
      841 tatgaggagt tggtctttcg gctcatctta atgaatctag tgatcatcgt gctgcagttt
      901 ttgagattgg cgccgcccgt cacgctgtgg ggaggcgtgt tgctaagcag tttcgtgttt
      961 gcgttggccc accatatcgg tccttatgga gaacccctcc aattgtttaa ttttctcttc
     1021 cgatttgtgg ccggtatttt ctttgcactc ctttttgtct atcggggttt tgggattgcg
     1081 gcaggttctc acgctgcata cgatctgatg gtggggctga tctaggagcc tatcccataa
     1141 tctctcgccg cccattcggc gggagagggg ccggaggtga gggtggctcg aatcgctgtt
     1201 cgttttcccg atcgactccg atgatggctt gccctcaccc caaccctctc ccagagggag
     1261 agggggactt tttgggattg ggataggctc taattgtctt cgggatgttt ccgcgcatgg
     1321 aaaaaacgcc gggggattcc cgccggagct atgaccatgg atcatctttg accggccttg
     1381 gcgggcggct tcttcgctta ttgcaccgga acaatgggaa catccacgac acggaatttg
     1441 atctcgtcgg aattggcacg aagctcgggg gcatacatgc cgtagccctt cgttggcagg
     1501 gccgagaatt taccggggat ttccgcccgc attcggtaac tgatgctctg tcgtcccctg
     1561 gggagggccc gacagaagaa gacaacccgg ttatcgcgga attcgacgta tgctcccgcg
     1621 gcatcgcgag tatagccgga gcgtaaatcg atgggttcga atccggccgg tttcatgtcc
     1681 tcgaagacca ggtattcata atcgttcttg ctttcgagga tgagttcaat ctccacgaga
     1741 tcaccacttt tcaattcggc caggttttga agcggctccc gttgatattt cgccacgcgc
     1801 tggtcgatga cctgtccgag agcaccggcc acctgggtcg tcgcttgaac gggcttcagt
     1861 cggtagtacc gccgatcaat tttgatttcc agaccggcgg ccgggatgaa atcctccagc
     1921 gtgaagtagc tcacgtaagc attcgcatag aggggccctt ggcctttgcg gcggagttcc
     1981 accacatgtt cgccggtggt cagggcctca ccggccagaa cgaaggtatt gtcaaaggcg
     2041 aagaggttct gcggtgtgat ctccacggcc ttcttcaact ctccgtcgat ccagacctcg
     2101 acggtcatat cgggcttggc ctccccgctg gcgcggatgt aatcggcgaa ggcctcgatg
     2161 accagggccg tgtcgcgggt ggagttccag tacgtggcgt gcttgcggtt attgagcaga
     2221 tatttgacaa gccgtggggc cagctcgccg cgggggtcgg ttcgggcgag caatttcagg
     2281 tagtaggcca tggcctcgat atcgttacca taccagtacc accagctcca tcgcccaagg
     2341 tttagccagg ccgtctgatt ttcgtcgtcc ttctccacgt actggttgat atttcgcaag
     2401 accatcgcca gtttctcttg gtcctgctgc ttatggagcg ccaacccgta catggccagg
     2461 gcatagaccg acagattaag ccggtcacgg tagaggtagt tcttcatttc ctcgttgcgc
     2521 accccggcat cgacaagcac catatagacg aacgcgtcga gattgtcggc gctagacttc
     2581 caagggacgt ttttaatttt gcggtcggca ttccgcagta actggagttg ctcctcctga
     2641 tacctttcaa gccaggccac accacgctga agcacgtcgg ggacgattgc cacatcgttc
     2701 tcctgcgcta attgcaggcc atggaccacg atggccgtaa gatggggtga agagaactcg
     2761 ccccaaccgg agaaccaacc ccaaccaccg tcagaaagtt gcatctcggt gagtcgttgg
     2821 acgccaacct tcacgatgcg gtccagttcg accttgctaa agacgggctc ccgccggtag
     2881 cgttgccact gtttctcgcg gtcttccggg gcgccaattt cttgggcgtt gagattgatc
     2941 cgtttgttac ggatgtcttc gaggtccaag ccaaggtcga tcaggacttt ctgtgtgatg
     3001 acggccggga ggaagcggtt gagcgtctgt tccgtgcacc cgtagggata gtcgattagg
     3061 tagggcaagg catcgaccat agcccccgcc agtgtgggcg agaagcggac ctcaaggcgt
     3121 gccgattccg gacggcgttc ggccgggatg aaaatcttaa attgaacagc ctcctgatcc
     3181 ggtctgatcc agccggagta ggggaccatc ttatccatgc cgtggacgta cgccgggaag
     3241 cgcatttcca tggcgtcaga ttcttcgtcg gtcagggccg ccatacggac aacggcctcg
     3301 ccttcggcct tgacccggac gcgccagtcc acgcgggctt catcgttggc agcgatgttg
     3361 atggtccgaa caagctcgtc gaggggttct agggtgttgc cttctagctc tagcctaacc
     3421 tgaaccgatt ttccctgctt gaggtagtta tggacgttgg cggagaggac aacctcatcc
     3481 gtttgcacaa agaatcgggg ggcttgaagg cgaatgatga ggttcttgcg ggtgaccacg
     3541 tcggaggcag cctcgccgac tttcgtgccg tgacccatgg cccaaacctt gatccgccag
     3601 gtggtcaggt tttcgggcat ggccagctca acctcggccg tgccatcggg tttggttgtc
     3661 aaagccccca cccagagggc cgtgtccgca aactctttac ggaccgtggg ggcgacaaga
     3721 gcctctgctt cttgcgggcc tgctcgatct gctccggctt ccgccgcatt catagcgggc
     3781 gcagcggcct tttccatggc cataccttct gccatcgccg ctcttggtgc tgcctccatt
     3841 ggcgcaccca tcatgaccgc gctacggcga acaatcccac ggccaccacc cccgaaggca
     3901 gcagactgca acacttcggg aacttcttcc accacagttg ccccaaagat gcctaagtct
     3961 tccatgcttg gctgattccg cggcggaatg tttccggaac tgcgcgacag cgaatgcatc
     4021 gtctgggagt ggtggtgccg acgccatttc cagaagaagg ccttgatttc tggcacgttg
     4081 gtcccgccgg aaatgtattc caccgacttg tcgtagattg ccacaactgt ggagccgacg
     4141 aagggttgtc ccgccaaatc ggtcagtttg atcttgacgc tcgccttttc gcccggcagg
     4201 tactctgtct tcgacggcgt gacttccaca ttgaggacac gtttttccgg tggcacgatg
     4261 atttccttga cctccgagta gagtttgcca tcgtaaaccg tgacggcttc cacgaagaaa
     4321 ttcggcatat ccttggccgt aacctcgatg ggcacaatcg tgcttttccc gtcgagagcg
     4381 agcagcttcg ggcgctgata gaccccgttg gcgggtcgca caaagagcag gactttggcg
     4441 tcgcgacaat tggcattgac ctgcaactgg accttttcgc ctggctgata ttccggcttg
     4501 tcgggaatca gctccagatc gttgaagcga aagtcctccg tggctcgcag gctttcgccg
     4561 atgatcgtaa agacgtagcc gccctcgatg gtatggcctt gttcatcggt gaccgtgtga
     4621 accagtcgaa actgtccggg ttccgcggcc ttgatttgca aacgggcctg gccctgctcg
     4681 tcagtcggca aatcccagct tcgggcctcg gtttcgacag gtttggcgtc cttgtaggtg
     4741 attctgaata aggagagctt tccctggcct ttcacgggtc ggccatcagg cgtcctggcg
     4801 cacgaatgca cctcaatcac atcacccgtg cggtagtagc cccgtggtag ccagagatag
     4861 accttgaagg gctgacgggt cactaggacc tctccggagc cgacaatggt gcgacgcgat
     4921 tggtcggtga cctccacagt gatcttgtaa cgatggtcct gatcaggatg gagcattttg
     4981 gccggcgccg tgtcgatcgt taccttcacg gtgccatcct cgccgatggc gacctcttgt
     5041 tcgccaatca actcaggcgg ttccggagac caatcaatcc agaaaggccg aggacgaaca
     5101 catccccaaa tatgccaccc ggggtaccag gtgtactcat aggcgaacca ccagtaaccc
     5161 ggaccatata gccaatcgaa ggggcgtggg ggataccaac tctcggtata gccagtccgc
     5221 tcgatcttgt atttgacctt ggcatgtgtc actggtgaac cgaagtagta ttttgcctgg
     5281 atggtggcct cgattttttc acccaaggcg atgggctttt ccggggcctg gacagtgact
     5341 tcaaactcgg gtttcttgta ctcctccaca cggaacgagc cgccgccgcg gaaccgcgaa
     5401 cccttaaccg ctagctgata cacacctagc gtggcatctt cagggatttc ccattcgccc
     5461 tcgataccac cgtattcgtc ggtcgtaacg ggcttggtcc acaggcgatc gccctttggg
     5521 ctgtgcaact cgagcacaca caattgattg gcgtacgccg aggtatccgg ttgatcgtat
     5581 ttcgcttcgc gcacccatag cttgtagtgg accttgtgtc ccgggcgata aaccggccga
     5641 tcggtgatgg taaagacctt cgtcgcgttg tactgtgcat cgtaatatcg gccgaaccac
     5701 aggtgtgtga agcccaggaa agccatccgg ccatcggcag cgcgggcgat gatcaaccac
     5761 tggtattctg tgggctgtat cttgggacca aggatgatct gcccttcggc atcggtgtat
     5821 tccgcgaagt tcttgatctc aatttgcggc cggggggcat caggtctccg ccatacctgt
     5881 tgccaaccga aaaactccac gttggccttg gcgatcggtt gacccgtgac ggcatcggcc
     5941 acataaaacc acgtgccgtt gtctagcggc ttttgggcga tgacggcgtc ggccacccaa
     6001 atgatcacaa agcaggtgtt gccgtttttc atttccgctt taagcaaata ggcccccgct
     6061 ttttgcaacg gcgttgcgat agtgacgcgc ttgtcgaaat ggttgggcct gggttccaag
     6121 gggacagtcc aggtggccac aacgggaccg agataccgag tttcggcctt ttcaatcaaa
     6181 cgatacccga tgttctcgat gttgatcttt tcccagttca attccagggg ttgagtcctg
     6241 atgtaatctt tgacgtcgct tagcagttgg tcgagtttga tggcgtaggc cgtcagtcgc
     6301 acctctgtcc cgttgcggaa gcgatactcg actgtcgctc cgcgtccggc tggctgcgtg
     6361 ctgatgggct caaaacgtcc ccaattaccc acgatttgat tgaggcgtgc ttcggcccat
     6421 gtcctctcct cgccctggag cagggggatg actttcttcc agtactcagc cgccttggga
     6481 tactgacgac gattctcaaa gatttgggca agctgcttaa gggcgtcgag cttgcgcgga
     6541 ctgcgatcct tctcggcgat cgttttccag atgcggatga aattgaactc gtcaggaagc
     6601 ttgaaacgtt tgatcccaat ggccaatcgg gcaatcgttt cgtcttcgcc caaggtgtgc
     6661 aaggcccagg ttccggtctc gctttcctga cgttcttctg tttccaagcg accaaagtac
     6721 caccagtagt cggccaacgt ctggacaccg aattgcgtcc aaaggaactc ggcaaacatg
     6781 taatcgactt cgtcggtcag tgcggcattc aattccttag cctggatcaa gcaccatcgc
     6841 caccgttcac cgtcgttttt cgcctgatca aacgttcgcg gtgccgcgta gaagatcggg
     6901 ttgccttcct catccacggg agctccccgc gtacccctgt ggaaatacca gccttcttca
     6961 taatcgggga gttggcttag gtcggttaag gcttgcaacc gccaggcctc tgaccaccct
     7021 cgatcgaaaa gaatgatgtt cgcaaaatcg aggtagaatt gcgccgcctc gtgtcggttc
     7081 ggttcttttt ccacaagggg catcgcttgc accagcagtt gcatggcacg gacgcggtct
     7141 ctctcttcgc acgagacggg cttgccctgg tcgccatcac taccccgttt aaactcattg
     7201 ccaataatca tgccgacatg gggaatctcc agataaagct tcgcagcggt cagcaaaaat
     7261 cgccaattct gggcatgaac gtccacagat ttttcgatga gcccatcaat gcgggagacc
     7321 tgattgagcc gggccaaaca ctgccaggca agactcaggt cattacaaac gcggaacgga
     7381 tccgcctttg ggtcggccag cgactcctcg aagagtcgca acgcctctcg aaattgcccc
     7441 tctttgaaca gtcggtttgc cgcttgccca cgttgtgcgt cgtccatcgt tgctcctttc
     7501 acaatttctg ctagacttgg ggtcacggaa agtagccagg ccaccctctc ataatgagac
     7561 cctatccacc ccaaacacaa cccgaagacc gccacccacg tccacgtctt agccgaagcc
     7621 cagttgcaac gaacctgcga cattgtattc cactccttgc ttaagcctca acacgcggac
     7681 tgagagttgt cgttttccct cagtccaaga taagaatcta tcccaaaaag tccccctctc
     7741 ccg
//
LOCUS       DAOSUT010000185         7628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4845_length_7628_cov_9.830846, whole genome shotgun sequence.
ACCESSION   DAOSUT010000185 DAOSUT010000000
VERSION     DAOSUT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4845_length_7628_cov_9.830846"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1797
                     /locus_tag="PLD05_13620"
     CDS             304..1797
                     /locus_tag="PLD05_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP78527.1"
                     /translation="MTTRVMLICFLLIGSLGLQGKACAEPARPNVLFIAIDDQNDWVG
                     CLGGHPLAKTPYLDSLAARGTVFLNAHVNSPLCNPSRTSLMLGLRPTTTGIYGLAPWF
                     RKVEALKDRVTLPQHFKAHGYRTYTAGKVYHGGVGGPAERAAEFDVWGPSPGIGVKPE
                     KKLIPPTPMGNHPLMDWGVFPHRDEDKGDYRLATWAIEQLRSMPRNQPYFLAVGFFLP
                     HVPCYVTQKWYDMFPNDDSVLPLVRDNDRDDTPRFSWYLHWYLPEPRLKWVKENNQWR
                     NLVISYLASTAFVDAQIGRILEALEETGLAKNTIVVVWGDNGFHLGEKGITGKNTLWE
                     RGTRVPLIFAGPGITPGQRCTQPVELLDIYPTLIELCGLTPRNDLEGISLVPQLRDAS
                     TKRDRPAITSHNQGNHAVRSERWRYIRYADGSEELYDMINDPHEWTNLAGRPEYASVI
                     EEHRKWLPKIDLPPAPGSADRVLTYDPATDEAVWEGMTIRRTDPIPE"
     gene            2008..3324
                     /locus_tag="PLD05_13625"
     CDS             2008..3324
                     /locus_tag="PLD05_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOP78528.1"
                     /translation="MRHVTEPSASIPGLLKKLSRRAEWAGGAPIANILMARTLAQPDV
                     ISLAAGFVEHNSLPVEWVEEAFRRIWANRSHALAALQYGTTSGYLPLREAILERLRQA
                     DGLSAAEHRASVDQMVITAGSNQLLYLLAEALLDPGDIVLCGAPSYFVFLGLIQSLGA
                     RTVGVATDYQGLIPEALEDRLDELERHGELDRVKAIYVASYFDNPTGVSLAADRRAAL
                     VEIAHRWSRRHRIYIIEDWAYRDLRYEGEDIRSVWTHDVSGETVIVTGSFSKSFSPGL
                     RVGWGLLPRSLLEPVLALKGHLDFGSANFNQVVLATVLQNGWFDKHVTRLRAIYHEKV
                     ATIVSALESYLPPILDVHYEAPKGGLYVWVRLPEEIDTGVDGPLFQAAVNEGVLYVPG
                     EYCFPAEGEPVRRHYLRLSFAMPDCDRLRCGVERLSRALRAVVQCD"
     gene            3401..4540
                     /locus_tag="PLD05_13630"
     CDS             3401..4540
                     /locus_tag="PLD05_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOP78529.1"
                     /translation="MMFKIIDRYIARYFLQSFFVCFFSLMCVYIVFDFFTNIDEFMRA
                     AEDSRQLAQLFFLYYGPQTLAVLDRILGLLVLTAAMFTVAWLQRQHEMTALLAAGISR
                     ARVAVPVIVGAVALIILGVINREVIIPRMIPLLSRSNTDLLGEKARDIQARYDNSTDI
                     LLRGGAAYRKDQRIAQPNFLLPPSLDQYGGQLIAENAYYLPPTADRPGGYLMDKMIRP
                     KNLHEKPSLSWKGRPVLLTPHDHPWLKPDQCFVVSDIDFEQLTGGRVWREYSSTLTLI
                     RGLHNPSLDLGADVRVRIHGRIVQPLRDLNLLFLGLPLVVRREQRNVFVALGLGVLVV
                     SLFTAIMLTCEYLGSIYLVSPALAAWLPFFIFVPLAVLQIDWLWE"
     gene            complement(5078..6688)
                     /locus_tag="PLD05_13635"
     CDS             complement(5078..6688)
                     /locus_tag="PLD05_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOP78530.1"
                     /translation="MKQEMLINAAQPEECRIAIVEDGVLEELYIERANQETYVGNIYK
                     GIVVNLEPSIQAAFVDFGVGRNGFLHVSDVEPQYFRQAGYDPEELGDLPPPASGVLNG
                     VEGGPARASRSRSSRNNIRPHVRPPIQNIFRRGDEVIVQVIKEGIGSKGPTLSTYISI
                     PGRYLVLMPALGRVGVSRKIEDEAVRRRLREIMHQLDLPKGLGFIVRTAGADRTRKEL
                     SRDLAYLLRLWKVIVRRIKKSEAPCDIYQESDMIIRTIRDIFSAEVGTIIIDEPSTYE
                     RAREFLEMVMPRYVNRVQYYDGKEPLFHKYGLEKEINIIHQRQVPLPGGGSIVIDQTE
                     ALVAIDVNSGNFRVDDSAEETAYQINLIAAKEIARQIRLRDLGGVIVNDFIDMRKEKH
                     RRAVERALREAVRRDRARTKILRTSPFGLIEMTRQRIRPSLKRTLYRDCPLCGGAGLV
                     KTVETMAIETMRQLLLAVLADRVAEITVHVHEEVAEFLNNDKRRFITDLETKHHVTIR
                     LVGEKHVSPEHLVIECRSSDGRIIPMPT"
     gene            complement(6728..7489)
                     /locus_tag="PLD05_13640"
     CDS             complement(6728..7489)
                     /locus_tag="PLD05_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOP78531.1"
                     /translation="MLSIAEQLDQIDQQGDRNRGPSASGSGGGNQSTVRQRVRIRFSK
                     SGELRFIGHHDLVRALERLFRRAQLPLAFSQGFHPKPKMSFPLALALGIEGREEVLEL
                     ELVEALSGEEILARLAQHPLPGLQFLSAEVLPSGTGKARVERVVYEISVPEDRLPMVQ
                     ARIDELRRRSELLLERPGQRSPRNYRSQLEDLSLHNNRLRMTIRVDPQGSVHPREVLG
                     LVGLDDLEADGYVLVRTKVELASEGRRHRGDGQSS"
BASE COUNT         1546 a         2135 c         2200 g         1747 t
ORIGIN      
        1 gatcggggtg aggatcgact ccaaagatcg cttgccctca ccccagccct ctcccaaagg
       61 gagagggaga tttgggatag gctccaagtt cgctggggct ttggtgttgg gatagtgaat
      121 gaattaagcc gtaatcgctc ggctccctcg cgttgtttga ccccttgtgc acgtgcaatg
      181 cgaggtggtt gaaaagtcgc gggaccgttt ggacatgtcg ctcgggcgag tcaggtgtgt
      241 gctcgtaagt taggatagcg ccttctactg ccatgggata gtgataatct tggggaggtc
      301 aacatgacaa cccgcgtgat gctcatttgc ttccttttga tcgggagtct tggcttacag
      361 ggaaaggcct gtgcagaacc ggcacggccg aatgtgttgt tcattgccat tgacgaccaa
      421 aacgactggg taggatgttt gggtgggcat cccctggcga agacacccta cttagacagc
      481 ttggccgccc gcgggacggt gtttctaaat gcccacgtca attcgcccct ttgtaaccct
      541 tcccgcacga gtttgatgct gggattacgc ccaacgacga cggggattta tggtcttgcc
      601 ccgtggtttc gtaaggtgga ggccttgaaa gaccgcgtca cgctgccaca gcattttaag
      661 gcccatggtt atcggactta caccgcaggc aaggtttatc atggtggtgt tggtggacct
      721 gccgagcggg cagcggagtt cgacgtttgg ggcccctctc ccgggattgg ggtcaagccg
      781 gagaagaagc tcatccctcc gacgccgatg ggtaaccatc ccttaatgga ttggggtgtg
      841 tttccccatc gtgatgagga caagggcgac taccgtttag caacatgggc cattgaacaa
      901 ttgcggagta tgccgcgaaa tcagccctac ttcttggcgg tgggcttttt cctgccccat
      961 gtgccgtgct atgtcacaca gaaatggtat gacatgtttc ccaatgatga cagtgtttta
     1021 ccgcttgtgc gtgacaatga tcgggatgat acgccgcggt tctcgtggta tctgcattgg
     1081 tatcttcccg agccgcggct gaaatgggtc aaagagaaca accaatggcg gaaccttgtc
     1141 attagctatt tggcaagcac ggcttttgtc gatgcccaga tcggacgcat tctcgaggct
     1201 ctcgaggaga ccggcctggc gaagaataca atcgttgtcg tctggggaga caatgggttc
     1261 cacctggggg aaaagggtat cacgggtaaa aatacgctgt gggagcgtgg cacgcgggtg
     1321 cccttgattt tcgcgggacc tgggatcacg cccggccagc ggtgtacgca accggtagag
     1381 ttgctcgata tttatccgac gctcatcgag ctgtgtggtt tgactccccg gaacgatttg
     1441 gagggaatca gcctggtgcc gcaactgcgg gatgcctcga cgaagcggga tcgcccggcg
     1501 attaccagtc ataaccaggg aaaccatgcc gttcgcagtg aacgttggcg gtatatccgc
     1561 tacgccgatg ggagcgaaga attgtatgac atgatcaacg atcctcacga gtggaccaac
     1621 ctggcgggcc ggccagagta tgcgtccgtc atcgaagagc accgaaagtg gttgccgaaa
     1681 atagaccttc cccctgcccc gggtagcgct gaccgcgtgc tgacttatga tcctgccacc
     1741 gacgaggcag tttgggaagg catgacaatc cggcgaacgg accccattcc tgaataaccg
     1801 tgacctgcgg gcaacgcctt tcggtaggat tgagccatgg gataatagga aacaccgcag
     1861 tcccgtgtgt cacgaacgcg gtcaattgcc gttgatcctc aagagtcttc gggtttacta
     1921 tgaacgctag cggcgagcga gggctaggcc atcccgagga gagtgctctt tcgttgcggg
     1981 tttcaagatg aagcacggtt tgaaaacatg cgtcacgtca cagagccatc ggcttccatc
     2041 ccgggtttac tgaaaaaatt gagtcgccgt gccgaatggg cgggtggtgc cccaattgcc
     2101 aatatcctca tggctcggac actcgcccag ccggatgtga tttccctcgc cgccggtttt
     2161 gtcgaacaca actccttgcc cgtggaatgg gttgaagagg cttttcggag aatttgggca
     2221 aatcgctctc atgcactggc tgccttgcaa tacgggacca cgagcggata tctcccgctt
     2281 cgcgaggcaa tccttgagcg tttgcgtcaa gcagatggtc tatcggcagc ggagcaccgc
     2341 gccagtgtgg atcagatggt gatcaccgcg ggaagcaatc agcttcttta tttgctggct
     2401 gaagcgttac tcgatcccgg tgatattgtc ctttgtggtg ccccaagtta ctttgtcttt
     2461 ctggggctga tccagagcct gggggcacga acggtgggcg tggctaccga ttaccagggg
     2521 ctgatccccg aggcccttga agaccggctc gatgaattgg aacgacatgg ggaacttgat
     2581 cgggttaagg ccatctatgt ggcgtcttat ttcgataatc caacaggggt gagccttgcc
     2641 gcggatcgtc gcgcagccct agtcgaaatt gcccatcgct ggtcacggcg tcatcgcatt
     2701 tacatcatcg aggattgggc ctaccgtgat ctgcgctatg aaggtgagga cattcgatcc
     2761 gtttggacgc acgacgttag cggcgagacg gtcatcgtca cggggagttt ttccaaatcc
     2821 ttttcgccag gtttgagggt aggttggggg ttacttcctc gctcattact cgaacccgtg
     2881 ctggccttga aagggcatct cgattttggt tcggccaact ttaatcaagt agtgctcgct
     2941 accgtccttc aaaacggctg gttcgataag catgtgacac gattgcgggc gatttatcat
     3001 gagaaggtcg cgacgatcgt ctccgccttg gaaagctact tgccaccgat cctcgacgtc
     3061 cattatgagg ccccaaaggg cggcctgtat gtttgggtcc gtttgccgga agagatcgac
     3121 actggggtgg atggtcctct ctttcaggcg gccgtcaatg aaggagtctt gtacgtgccg
     3181 ggagagtatt gttttcccgc ggaaggagag ccggttcgtc gccattactt gcgactcagc
     3241 ttcgccatgc ctgattgcga ccgcctccgc tgcggggtag aacgactatc gcgggcactt
     3301 cgggcagtag tacaatgtga ctgaacgcca cgacaggatt ctccccgatt tggcaatctg
     3361 ggaaacttaa ccaaaccgtc acggatggtg atatgtttgt atgatgttca agatcattga
     3421 ccgctacatt gctcgctact tcctccaatc cttcttcgtc tgctttttca gcttgatgtg
     3481 cgtttacatt gtgttcgact tctttacgaa catcgacgaa ttcatgcggg cggcagaaga
     3541 tagcaggcaa ctcgcgcaat tgttcttcct ttactatggt ccgcagacgc tggccgtgtt
     3601 agaccggatt ttaggcttgc tggtcctgac ggccgccatg ttcacggtgg catggcttca
     3661 gcggcagcac gagatgacgg ccctgctagc ggccgggatt tctcgtgccc gggtcgcggt
     3721 acccgtcatc gtgggggctg tggcgctcat catcttaggc gttattaacc gggaagtgat
     3781 catcccccga atgatccccc tcctttcgcg gtccaacact gatttattgg gagaaaaggc
     3841 gcgggatatc caggcacggt acgataactc gacggatatc cttttgcggg gtggggcggc
     3901 ctatcgcaag gaccagcgaa ttgcccaacc aaacttcctc cttcctccca gtctcgatca
     3961 gtacggggga caattgattg ccgagaatgc ctattactta ccgcccacag cggatcgccc
     4021 cggcggatac ttgatggaca agatgattcg gccgaaaaac ctccacgaaa agccttccct
     4081 ttcgtggaag ggtcgtcccg tccttttgac gccccacgat cacccctggt taaagccgga
     4141 tcagtgtttt gtcgtcagcg acattgattt cgagcaactc accgggggac gcgtatggag
     4201 ggaatattca tcgaccttga cactgatccg gggactacat aatccgagtt tggacctggg
     4261 ggccgatgta cgggttcgca ttcacggacg gatcgtccaa ccgctccgcg acctcaatct
     4321 cctcttcttg ggtttgccgc ttgtggttcg ccgcgagcag agaaacgtgt ttgtggccct
     4381 ggggctgggg gtactcgtgg tcagcctctt cacggccatt atgctgacgt gtgaatacct
     4441 cggttcgatt tatctcgtct ccccggcctt ggcggcttgg ttgccgtttt tcatctttgt
     4501 gccgctggct gtgctccaaa tcgattggct ttgggaataa gtgcctatcc caaaaactcc
     4561 ccctctccct ccgggagagg gttggggtga gggcagggag ggacgcggtc ccccgcgtcc
     4621 gtcatttaga gcctatccca aggttccctc ttcccttgag agcggattgc ggtgagggca
     4681 agcgaccgcc ggaatcgatt gagacaaccc gcagcaattc gagccaccct cacccccggc
     4741 ccatctgtcg ccaaacgaat gacgggagac taggggatag gctctaagat gccgcaatgg
     4801 ccttgagggg gtcgccgtcc ccggcgacca atttggctcg gggattggtt gtgtttaggg
     4861 gcaggcaccg gggcctgccc ctacgggttt caatgggttc ggcaaccgta ggggcaaccc
     4921 cccgtggttg ccccgtttgg ccgtagcgcc ggtgcacacg ggacggcgag gacgccgtcc
     4981 ctcccaatcg cctagcgagg gtcatcgtcc ccgatgaccc aaattaccgg gagggtcgcc
     5041 gtcctcggcg accagatcgt tggacggcgg ggccgattca cgtgggcata gggataatac
     5101 gtccatcact gctacggcac tcgatcacga ggtgttccgg cgatacgtgt ttttccccga
     5161 ccagcctaat ggtgacgtga tgcttcgtct ccaaatcggt gatgaagcga cgtttgtcgt
     5221 tgttgagaaa ctcggccact tcctcatgga cgtgaacggt aatctcggcc acacgatcgg
     5281 ccaggacagc caaaagcagt tgacgcatgg tctcgatagc catcgtttcc acggtcttga
     5341 ccaggcctgc accgccgcac aaggggcagt ctcggtaaag cgtgcgttta aggctggggc
     5401 ggatgcgctg ccgcgtcatc tcaattaggc cgaaggggct ggtccgcaaa atcttagtcc
     5461 gtgcccgatc tcgccgcacg gcctcgcgga gggctcgctc cacggcccgc cggtgttttt
     5521 cttttcgcat atcgataaag tcgttgacaa tcacgccccc gaggtcgcga aggcgaatct
     5581 gccgagcgat ttccttggcc gcaatgaggt tgatctggta ggccgtctcc tctgccgaat
     5641 catccacccg gaagtttccg ctgttcacgt cgatggccac gagggcctcc gtctggtcga
     5701 tcacaatgga cccaccgccg ggcagtggca cctgacgttg atgaatgatg ttgatctcct
     5761 tttctaggcc gtacttgtga aagaggggtt ccttcccgtc gtaatactgc acccggttga
     5821 catagcgggg catgaccatt tccaagaact ctctggcccg ctcgtacgta gaaggctcat
     5881 caatgatgat cgttccgacc tccgcactga agatatcgcg gatcgtgcgg atgatcatgt
     5941 cgctttcttg gtagatatca cagggggctt ccgacttctt gatgcgacga acgatgacct
     6001 tccacagacg caggagatag gccaggtcgc gggacaactc cttgcgggtg cgatcggccc
     6061 cggccgtccg cacaatgaac cccagcccct tgggcaggtc aagctgatgc ataatctcgc
     6121 ggagtcggcg acgaacggcc tcgtcttcga tcttgcgcga gaccccgact cttcctaaag
     6181 ccggcatgag aacgagatac cgaccaggga tactaatgta ggtggaaagg gttggccctt
     6241 tgctgccaat gccttcctta atgacttgga caatgacctc gtcgccgcgg cgaaagatgt
     6301 tttgaatggg tggacgcacg tgcggccgga tattattccg cgaggaccga ctgcgagaag
     6361 cccgcgccgg cccgccttcc actccgttga gaacccccga agcgggcggt ggcaaatcgc
     6421 ctagctcttc cggatcatag cccgcctggc ggaaatattg cggctcgacg tcgctcacat
     6481 ggagaaaacc gttccggcct accccaaagt caacaaaggc cgcctgaatg ctcggctcca
     6541 ggttgacgac aatcccctta tagatgttgc caacgtaagt ctcctgattt gcccgctcga
     6601 tatagagctc ttcgagaact ccatcttcca caatggcaat ccggcattct tccggttgcg
     6661 cagcattgat taacatttcc tgcttcataa actcgccatc ctttgcggca ctggaggcga
     6721 gtgcgcatca gcttgattgg ccgtcccccc gatgacgacg cccttcggag gctaactcga
     6781 cctttgtccg cacgaggaca taaccgtctg cttccaaatc atccagcccc accaatccga
     6841 ggacctcacg cggatgaacc gaaccttgcg ggtccacacg gatggtcata cgaagccggt
     6901 tattgtgcag agacaaatcc tcgagttgcg aacggtaatt acggggcgaa cgctgacccg
     6961 gtcgttccag cagcaactcg ctgcgacgac gaagttcatc gatgcgagcc tgaaccatcg
     7021 ggagacgatc ttcgggtacg gagatttcat ataccacgcg ttcgactcgc gctttgcccg
     7081 tgcccgacgg taggacttcg gcagacaaaa actgcaagcc cggaagggga tgctgcgcga
     7141 gtcgggccaa aatctcctcg cccgaaagag cctcaaccaa ctccagttcg agcacttcct
     7201 cgcggccttc gatgcccaat gccaacgcca gaggaaagct cattttcggc ttgggatgaa
     7261 atccctggct gaaggccaat ggcagttgtg cacgtctgaa aagccgttct aatgcccgaa
     7321 ccaagtcatg atgaccgata aatcggagct cgcccgattt ggaaaagcga atgcgcactc
     7381 gttggcgaac cgtagattgg ttcccccctc cgctgccgct ggcggagggg cctcggttcc
     7441 gatcaccttg ttgatcgatt tgatccaatt gttccgcgat acttaacacc cgtagatacc
     7501 taaagctcct tcccggagag cggcattcgg agtgaccacc caagatagtt ccaaccgaac
     7561 gccggtccct gggtttgatc gcttgctacc ttcacgagac aaccgctgcc gtcctgcggc
     7621 tgcttcgt
//
LOCUS       DAOSUT010000186         7530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4891_length_7530_cov_7.693512, whole genome shotgun sequence.
ACCESSION   DAOSUT010000186 DAOSUT010000000
VERSION     DAOSUT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_7530_cov_7.693512"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2664
                     /locus_tag="PLD05_13645"
     CDS             <1..2664
                     /locus_tag="PLD05_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOP78532.1"
                     /translation="FEGEKRAVVIAQALVETVGKKVNKRYQILKTVRGASLVGKRYIP
                     PFDYYYKRYGEQQGRLRGGGQQHIAWRVVAADFVTIDTGTGVVHEAPAFGEVDYEVLL
                     AEQARFVEGEGPQLICAVAPDGTFTAEASDYAGRWVKDCDRDIIRQLRQSGVLFHFEQ
                     YVHDYPFCWRAENDPLIQYPRKSWFIRTTAFKEKMLENNRQIGWHPENIRDGRFGNFL
                     ETNVDWALSRERFWGTPLPIWVCEKTGYMEAIGSYAELLAKPGVSGMEVWEEAKRADP
                     TLSEHLKVHKPYIDAIVYDSPVCPGSKMRRVPDVIDCWFDSGAMPFAQWGYPHVPGSP
                     EKLAQQFPADFISEAIDQTRGWFYSLLAISTLLFSPDENRPGGSAEGFLRERTPAIAF
                     PHPYRNCVVLGLMLGEDGQKMSKSKRNYREPQEIFDRYGADALRWYFFANQAPWTSIR
                     YNEQAIRDSIPEFLLRLWNVYSFLVIYARIDGWDPAATLKGTVGDLRHEVLKQASDYR
                     PPRERCELDRWILSELHRTISAVIRFMDDYDNYSACQQILAFVDALSNWYVRRSRARF
                     WASEWTDDKRDAYWSLYECLITLCRLVAPFVPFITEEIWQNLAVNVFGDRTIPSVHLC
                     DYPKPEPDLVDEELSAWMGAVRELVSLGRAARMNAKLKVRQPLERVEIILADRDQLPA
                     LQQHTELIAEELNVKKIEWRERADDLIDYTVLPDFKRLGPRLGPRLPAVKQWLSQAEG
                     SSLLQQLETIGQVIVPLPDSEISLTKEDIQIRLNAKPGWTAAQGRVGVVILSTQLSPA
                     LIAEGLARDFVHAVQNRRKELDCDFVERIEVGVVTQSESIKQAIRDYQDYIMAETLAT
                     SLQFSPLTGTTPTTLNIGQQQVELYVKPVRN"
     gene            3057..4778
                     /locus_tag="PLD05_13650"
     CDS             3057..4778
                     /locus_tag="PLD05_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78533.1"
                     /translation="MPSLFVIRGNDQGTRFELEGSTITIGRDSSNQVQLHDTGVSRHH
                     AEIKRVENRFLLVDLNSSNGTLVNGKRIQEHWLTSGDQIQMGRTLMLFTSPAEDAQPE
                     TPWNVAISSEPDEDRSQIVSALKQEEGSRFFANAVVGNDPWTVQARRNLEIMYHTALA
                     ISHTVDIDQLLNRIMDLIFQWVEADRGCIMLIDPETNRLVPKVLRARKGAERPGKLII
                     SQTILDYVRERNEGVLTSDARHDERWDSGASIFQAGIREAICVPMQGRYGVVGVIYID
                     TSMSPQEMVRRGNTNKFTEDHLRLMIAIAHQAALAIEDTHYYSAMVQAERLAAIGQTI
                     ATLSHHIKNILQGIRGGSYLIEMGLNKHDEELIRKGWTIVEKNQAKISGLVMDMLTFS
                     KEREPDLQPADLNQLIADVIELMQSRAEECNVELTWKQEGPIPVLIFDPEGIHRAVLN
                     LVTNAIDATAERPQPRKVEITTRYDEAQGKVWITVQDNGPGIPPELLDKIFSPFVSVK
                     KGTKGTGLGLPVSQKIVQEHGGRLSVESVIDQGTTFTIELPAIRPAEAGPVESSATGE
                     MHALPPE"
     gene            complement(5035..5340)
                     /locus_tag="PLD05_13655"
     CDS             complement(5035..5340)
                     /locus_tag="PLD05_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOP78534.1"
                     /translation="MLPIGQFSLPGGTEMIIILVVALLLFGNRLPQVARSLGKSLNEF
                     RRGMQGLEQELKSAINETPPPATPKKRAENEEDRQTPVAPKFIPPSEPPQETVESQS"
     gene            complement(5420..5632)
                     /locus_tag="PLD05_13660"
     CDS             complement(5420..5632)
                     /locus_tag="PLD05_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOP78535.1"
                     /translation="MLAFMFGAPGWMEMLVIALVILLLFGNRLPSVMRSLGRGVVEFK
                     KGLSDQGSDEEHEEKAVTKEDAKAEK"
     gene            6159..6674
                     /locus_tag="PLD05_13665"
     CDS             6159..6674
                     /locus_tag="PLD05_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78536.1"
                     /translation="MKRREFRIMVSATRRHLVLMMVTGLLVGGMSLSAQAGLICSVSG
                     DGQSFDQLATHSSLPFSLVLDLCLNDDPGPGMVVPDVSDDGSKNPTQPMLPFFVLLNA
                     DLIAGMQAASQSGMSGVSSTSGPQSNSASFSAVLSADLELADPPLRDRLLDEPRLVLP
                     LGPPFELLRPA"
     gene            6777..>7530
                     /locus_tag="PLD05_13670"
     CDS             6777..>7530
                     /locus_tag="PLD05_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78537.1"
                     /translation="MRSKLAVNILIAICVLASGPLAWADWMSEYCGYSAFASNRDAAV
                     TYETDGYVSFAVWDRGSPSGDLPTLPRGAEYMVWIGSTFERGTPTGRYIYFYQVVNAQ
                     DGRSALKDFRIFDLGFIQVGFVDNAVFQDSQGPVLGSGNVALDNQTSGHPIRGDDQVD
                     GKPTLSGIPPAGLANANGNSVAPTSIDVNVNSGYTQWYFVRGNQGIRLDQANTSYLLF
                     ATSDLRPTYKASEERDGNTANCDVPATPEPGSL"
BASE COUNT         1687 a         2181 c         2000 g         1662 t
ORIGIN      
        1 tttgaaggcg aaaaacgagc cgtcgttatt gcccaggccc tcgttgagac ggtgggtaaa
       61 aaggtcaaca agcgctatca aatcctcaag acagtgcgcg gcgcgtcgtt ggtaggtaag
      121 cgatacatcc ctccgttcga ctactactac aagcgctatg gagaacaaca gggacgcctc
      181 cgtggcggcg gtcaacaaca tattgcctgg cgggtggtgg cagccgactt cgtaaccatc
      241 gacaccggaa cgggcgtcgt tcacgaggcc cctgcttttg gggaggtgga ctatgaagtt
      301 cttttggcgg aacaggcccg ctttgtggag ggtgaaggtc cccagttgat ttgtgccgta
      361 gcccccgacg gcacgttcac cgccgaagcc tccgattacg ctggtcgctg ggtcaaagat
      421 tgcgatcgcg acattatccg tcaactccgg cagtcgggtg tccttttcca tttcgagcag
      481 tatgtccacg actatccctt ctgttggcgg gccgaaaacg atcccctcat tcaatatccc
      541 cggaagagct ggtttatacg caccacagcg ttcaaagaaa agatgcttga aaacaatcga
      601 cagattggct ggcatcccga aaatatccgg gacggtcgat tcggaaattt tctggaaacg
      661 aacgtcgatt gggccttatc ccgcgaacga ttctggggaa cacccctccc catttgggtc
      721 tgcgaaaaaa cagggtacat ggaggccatc ggttcttatg ccgaactcct tgccaagcct
      781 ggtgtcagcg gcatggaggt ttgggaggag gcaaaacggg ccgatcccac cctctccgag
      841 catctcaagg tccacaaacc ctacatcgat gcaattgttt acgactctcc ggtttgcccc
      901 gggagcaaaa tgcgtcgtgt tcccgatgtc attgactgct ggttcgattc cggtgccatg
      961 ccctttgccc agtggggata cccccatgta ccggggtccc cggaaaaact cgcccagcag
     1021 ttcccggccg attttatcag tgaggccatc gatcaaaccc gcggttggtt ctatagcctt
     1081 ctcgctatca gtaccctcct ttttagcccc gacgaaaaca ggcccggcgg ctcggcagag
     1141 ggctttctcc gagaaaggac tccggccatt gcgtttcccc atccgtaccg gaattgtgtc
     1201 gtcctcggcc tgatgctggg agaagacggc caaaagatgt ccaaaagcaa gcgcaattac
     1261 cgagagcccc aggaaatctt tgaccgctac ggggcggacg ccctccgctg gtacttcttt
     1321 gccaaccagg ccccatggac atccatccgc tacaacgaac aggccattcg agacagcatc
     1381 cccgaattct tgctgcgatt gtggaatgtc tatagcttct tggtgatcta tgcccgcatc
     1441 gacggttggg acccagctgc caccctcaag gggaccgttg gcgacctacg tcatgaggtc
     1501 ttgaagcaag cctctgacta tcgcccgccc cgtgagcgct gcgagttgga tcgttggatt
     1561 ctcagcgagt tgcatcgcac catttccgcg gtaatccgat tcatggacga ctacgataat
     1621 tactcggcct gccaacagat ccttgccttc gtggatgccc tttccaactg gtacgtccgc
     1681 cgcagtcgag cacgcttttg ggcaagtgag tggaccgatg acaaacggga tgcctactgg
     1741 tcactctacg agtgcctgat tacactctgc cgccttgttg ccccctttgt ccccttcatc
     1801 accgaggaaa tttggcaaaa cctggcggtt aacgtctttg gtgaccgtac tatcccaagc
     1861 gttcacctct gtgactatcc caagccagag ccggaccttg tggacgaaga gctttccgcc
     1921 tggatggggg cggtccgaga actagtttcc ctcggtcggg cagctcggat gaacgctaaa
     1981 ctgaaggtcc gccagccttt ggaacgtgtc gagatcatcc tcgccgatcg cgatcaactc
     2041 cccgccctcc agcagcacac cgagctgatt gccgaagaat tgaacgtcaa gaagatcgag
     2101 tggcgcgaac gggccgatga cctcatcgac tacaccgttc tcccggactt taaacggctc
     2161 ggtccccgcc tggggccccg actccctgcc gtaaagcaat ggctttctca ggcagaagga
     2221 agcagcctcc ttcagcaact ggagacgatt ggtcaagtca tcgtgccctt gcccgatagt
     2281 gagatttcct tgaccaaaga ggatattcaa atccgcctca atgccaaacc gggttggaca
     2341 gcggcccaag gccgggtggg cgtggttatt ctctctaccc aattgtcacc agcactcatc
     2401 gccgagggcc ttgcccgcga tttcgtccac gcggttcaaa ataggcggaa ggaactcgat
     2461 tgcgatttcg tcgaacgaat cgaagtcggt gtcgttacgc aatcggagtc gatcaagcag
     2521 gccatccggg actaccaaga ctacatcatg gccgaaacct tggccacatc gcttcagttt
     2581 tctccgctca ccgggacaac gcccacaacg ttgaacatcg gtcaacagca agtcgaactc
     2641 tatgtcaagc ccgtgagaaa ctaacccgag cgtatcggcc ttcgaccgac gtgatgtttc
     2701 caagactagc gtcaccaagg cattagttta gagtctttcc caaaaagtct cccctctccc
     2761 tcggggagag ggttggggtg agggcaagcg aacgtcggaa tcgatcgaga aaaccggcag
     2821 cgattcgagc caccttcacc cccgactctt ctagcgccga acgggcgagg ggagagtatg
     2881 ggacatgctc ttaatgacct ccggcgatcg cggtagtcct ggcatgacaa caaaacaggg
     2941 gtgtcacacc agccctcgaa tccttagaat acaagaggat actggcggct gtggcgttaa
     3001 cccactgatt gttacggaaa acaagtgccc aattccacaa ggggggacac cgagtcgtgc
     3061 catccttgtt tgtgatccgc ggaaatgacc aagggacccg tttcgagctg gagggctcaa
     3121 ccatcaccat tgggcgagac tccagtaatc aggtccagct ccacgatacg ggtgtctccc
     3181 gccaccatgc cgagatcaaa cgggtggaga atcgcttcct tttagtggac ctcaatagct
     3241 ctaacgggac gctcgtcaac ggaaagcgga tccaagaaca ttggctgacg agtggcgacc
     3301 aaatccaaat ggggcgaacc ctcatgctct tcacctcccc ggcggaagac gcccagccgg
     3361 aaaccccgtg gaacgtcgcc atttcctctg aaccggacga agaccgctcc cagatcgtca
     3421 gcgcccttaa gcaggaggaa ggtagccgct ttttcgccaa cgccgttgtg ggcaacgacc
     3481 cctggaccgt ccaagcccgt cgcaacctgg aaatcatgta tcacacggcc ttggcgatca
     3541 gtcacaccgt tgatatcgac cagctcctca atcgcatcat ggatctgatc ttccagtggg
     3601 tcgaggccga ccgcggctgt atcatgctca tcgatcccga aacgaatcgt ttggtgccca
     3661 aagtccttcg ggcacgcaag ggagcagaaa ggcccggcaa gttgatcatc agtcaaacca
     3721 ttctcgacta cgttcgagaa cgcaacgaag gcgtcctcac cagcgacgcg cgccacgatg
     3781 aacgttggga ttccggggcc agtatcttcc aggcaggcat ccgcgaggct atttgcgtcc
     3841 ccatgcaggg acgttatgga gttgtcggcg tcatctacat cgacacctcc atgtctcccc
     3901 aggagatggt ccgccgcggc aacacgaaca agtttacgga agaccatctc cgcctcatga
     3961 tcgccattgc ccaccaggcg gccctggcca ttgaagatac ccactactat tcggccatgg
     4021 tccaggcaga gcgactcgcc gccattgggc agaccatcgc caccctatct caccacatca
     4081 aaaacatcct ccagggtatc cgcgggggca gctacctcat tgaaatgggc ctcaacaagc
     4141 acgacgagga actcattcgc aagggatgga ctatcgtgga aaaaaaccag gccaagatct
     4201 ccggcttggt catggatatg ctcaccttca gtaaagaacg cgaaccggac cttcagccgg
     4261 ccgatcttaa tcaattgatc gccgacgtca tcgaactcat gcaatcccgg gccgaggagt
     4321 gcaacgttga attgacctgg aagcaagagg gccccatccc ggttctcatc ttcgatccgg
     4381 aaggcatcca ccgggcggtg ctcaatttag tgaccaacgc catcgacgct accgccgaac
     4441 ggcctcagcc gcgcaaggtc gagatcacaa ctcgttatga cgaggcccag ggaaaagtct
     4501 ggatcaccgt tcaggacaat ggcccaggaa tcccgcccga gttgctcgat aaaatcttta
     4561 gtccgttcgt ctccgtcaag aagggcacca aaggaacggg cttgggcctc cctgtcagcc
     4621 aaaagattgt ccaagagcac ggcggacgtc ttagcgtcga atccgtcatc gatcaaggga
     4681 cgaccttcac catcgaactc ccggccattc gccccgccga agccggtccc gtcgaaagct
     4741 cagcgaccgg cgaaatgcat gcccttccgc ctgaatagat ggggcgcata aaggttggcg
     4801 aaagagggca atttgggact tgcttcagtt cagagcctac tccacaatct cacctcaggc
     4861 gttcagcgga agaggggtcg gcactgagcg tcgctcgaat cgctgcctgt tttatcgatc
     4921 gactccgacg attgcttgcc ctcaccccaa ccctctcccg gagggagagg ggatactttt
     4981 gcggatggac tgtgaaattt actgcctaag tgtggcccta gtgtgacgag gcaattatga
     5041 ctgtgactcg acggtctctt gaggaggctc gctgggagga atgaatttcg gggcaactgg
     5101 ggtttgacgg tcttcctcat tctcggcccg tttcttgggt gttgcgggcg gaggtgtctc
     5161 gttaatcgcg gacttgagtt cctgttctag cccctgcatc ccccgacgaa attcattcaa
     5221 gctctttccc aaagagcgcg ccacctgagg aaggcgattc ccgaacagca aaagcgccac
     5281 cactaggatg atgatcatct ctgtgccgcc cggcaaggaa aactggccta tcggcagcat
     5341 atcgcatcac ctcccttctc gccgcaaaac cgttccaggc tggaccgtgg tgaacgcccc
     5401 cacggaaaac ctaaacacct cacttctcgg ctttggcatc ctctttggtt actgccttct
     5461 cttcatgttc ctcatcgctt ccctgatccg atagcccctt tttgaactcc accacacccc
     5521 ggcctaacga acgcatcaca ctagggagac gattcccaaa tagcagcaaa ataaccaggg
     5581 caattaccag catctccatc caacccgggg caccaaacat aaaggcgagc atacacaagc
     5641 tccttatgag atcaaaacct ggcagacatt ctgccaacgg cgtttgcaca cgtcgagcat
     5701 ccacacccac ttcgtccatt ttaccccgcc cgccataaaa actgtagggg cagccttgta
     5761 gctaccgcgt aagcctcacc acccacgatt gcaaggcgtg ggagtcgctt cagcatcctg
     5821 tccgttcgtg atatacccgc tgccgccgtg gtctcccgtg ctcggtatat gagcccattt
     5881 ccatccacaa cgcagctcct cgtgctgtcc ttgccgtctc accgttattc ggaaaaattt
     5941 ccccgacccc ctcaatcggg ttattgacaa ggtttttcgt tactgtatcc taaaaccaag
     6001 gttcagccgc agagtcgtcg tacgatcggt gcggtagccg agaaatcaga aaattccgat
     6061 tttagggtat tgacaacgca aagagcctgt gtattatgga atcaaattca ggacggagcc
     6121 gggatttgca ttgttaagcg tcgaaggatc aaggggcttt gaagcgacgt gagtttcgca
     6181 tcatggtgag cgcgacccgg cgacatctag ttctgatgat ggtcaccggc cttttggtcg
     6241 gcggaatgtc gctctctgcc caagcggggt tgatctgttc ggttagtggc gacgggcagt
     6301 cgttcgatca gcttgccacc cactcctcgt taccgttttc tctggtgttg gacctttgct
     6361 tgaacgatga ccccggtccc gggatggtgg tgcccgacgt gtctgacgac gggtcaaaga
     6421 accccactca gccgatgttg ccatttttcg tacttttgaa tgctgaccta atcgctggaa
     6481 tgcaggcggc ctctcaaagc gggatgtctg gcgtttcttc tacgagtggt ccccagtcca
     6541 atagtgcatc tttctcggcg gtcctgtcgg cagatttaga acttgccgat ccaccgttgc
     6601 gcgaccgcct gcttgacgaa cctcgtctcg ttttacccct cggcccaccc ttcgagctcc
     6661 tcagacctgc gtaggttcat tttgatagcg gtcttgcgga cttgtgtgct atcgagaggt
     6721 ttgaggttta gtaacgggtg gttttgaaaa gagcaatcag aatgatgagg tgacatatgc
     6781 gttctaaatt ggcagtaaat attttgatag cgatatgcgt cttggcttca gggcctttag
     6841 cttgggcaga ctggatgtcc gagtactgcg gttatagcgc ttttgcttca aatcgcgacg
     6901 cggcagttac gtatgagacc gacggctatg tgagcttcgc ggtatgggac agagggtctc
     6961 cgagcggtga tttgccgaca ttgccaagag gtgctgagta catggtttgg attggctcaa
     7021 cttttgaaag gggtactcct acaggccggt acatctattt ttaccaggtg gtgaatgcgc
     7081 aggatgggcg tagcgcgttg aaggacttca ggatcttcga cctcggtttt attcaggtcg
     7141 ggtttgtcga taatgccgtt tttcaggatt ctcaagggcc agtgcttggg agcggtaatg
     7201 ttgccctcga caaccagaca tctggccatc ctattagggg agacgatcag gtcgacggca
     7261 agccgacgct gtcgggaatt ccgccagcgg gattagctaa cgctaacgga aattcagttg
     7321 cgcccacctc tattgatgtt aatgttaatt caggttacac ccaatggtac tttgttcgcg
     7381 ggaatcaggg gatacgactc gatcaagcta atacgagcta cctattgttc gcgacatcgg
     7441 atttgcggcc gacatataag gcatcggagg agcgagatgg taatactgcc aattgcgatg
     7501 tcccggcaac gccagagccg gggtcactgg
//
LOCUS       DAOSUT010000187         7505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4908_length_7505_cov_8.815839, whole genome shotgun sequence.
ACCESSION   DAOSUT010000187 DAOSUT010000000
VERSION     DAOSUT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4908_length_7505_cov_8.815839"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLD05_13675"
     CDS             complement(<1..497)
                     /locus_tag="PLD05_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HOP78538.1"
                     /translation="MFESTTPSQSDPASPSGSPSKSVWVPTRPELTEDDKENVLRRAA
                     ECVLSAIVGQDKPISEGQSPAVPAVPVWGVFVSLKSGQQLRACCGHIEDEVLLPTALE
                     RAAHIAATWDLRFPAIQPAELSLLDLEVWILWNRQRIEAQGESRVKSIEIGKHGVQVV
                     KGQQRG"
     gene            complement(632..1756)
                     /gene="amrS"
                     /locus_tag="PLD05_13680"
     CDS             complement(632..1756)
                     /gene="amrS"
                     /locus_tag="PLD05_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOP78539.1"
                     /translation="MARHVELPPVAAATADGICPAGWWHEEDGKIVCDLCPRNCRLGE
                     GQRGFCFVRQNLNGEMVLTTYGRSTGFCIDPIEKKPLNHFYPGTAVLSFGTAGCNLGC
                     KFCQNWSISKSREIERLSEIALPKAIARAAQTLGCTSVAYTYNDPVIFAEYAVDTAKA
                     CREVGIKNVAVTAGYISSPAREYFFEYMDAANVDLKAFSERFYKELCSAKLQPVLETL
                     KYLVNKTSVWVEITNLIIPQENDDPTEIRQMCRWIVDELGPDVPLHFTAFHPDFRLVD
                     RPPTPAEKLIEAYEIARDVGLHYVYTGNVLDTVRQSTYCPHCGQLVIERNGYWIGRYG
                     LQGNRCRYCGSEIAGRFSTAGAPGTWGARRYPVRISEFAK"
     gene            complement(1825..3114)
                     /locus_tag="PLD05_13685"
     CDS             complement(1825..3114)
                     /locus_tag="PLD05_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOP78540.1"
                     /translation="MPDWPSGILFSETTPLSMPALFGRLGMAALLGFLVGMQREHTEG
                     GMPGLRTFPLITLSGSVFAILGTWFGGWLPAAALVCLVALLFFPHWLRIQRAEPDPGL
                     TTSVAVILMYGVGALLVIGPLEIGVVMGGAVAVLLQFKPELHRISERLKDEDVRAIMQ
                     FVLITCIILPVLPRNTVDPLGVISPFNVWMMVVLIVGISLSGYIAYKFLGQRATILLA
                     GILGGAVSSTATTISAARQSQENRQDEVSTIIIILLASTVMFARVFVEILVVHPGMLH
                     HCVVPLALMSVATYLPVPFLWRRVRDQQLQVVPSHRNPTQLRAALFFAALYAIVLFLI
                     AWIRQHVGASGLYPLAVLSGLHDMDAITLSISRMALQDQDLSLNGWRYIAAAILANMG
                     VKSLLGGIMAYPRLGLRLCLGFLPGFLVGLGLILFWP"
     gene            complement(3179..3601)
                     /locus_tag="PLD05_13690"
     CDS             complement(3179..3601)
                     /locus_tag="PLD05_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78541.1"
                     /translation="MAQERISDQELEAYLDEALPPERMVAIENALRHNHECLLRLRRI
                     VSRRDNGIHSVAEIWRRNRLSCPSREALGAYLLGALTEPESTYVKIHLETVGCAICLA
                     NLEDLRAQGVPSDGERAEIQRRRQKYFQSSIGHLSRRE"
     gene            complement(3635..4288)
                     /locus_tag="PLD05_13695"
     CDS             complement(3635..4288)
                     /locus_tag="PLD05_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOP78542.1"
                     /translation="MAEQTSDSERHLISRVRSGDPEAWKEVIRRYEGRLYSYLGSHVK
                     HRDIAEELVQETFVGFLVSLPNYDSRRSLENWLFSIAAHKLTDYYRRQGRRITLSLEE
                     GIEGANRCAIGGRPQRGPSTVAASHERRGIEEQALSETIEKQIMAWKSQGEWLKVSCM
                     ELLFLCGWSNKETAQYLHIDEQQVANWKFDFLRRIRERLRAQGLPQDVFPELQGEEK"
     gene            complement(4425..5423)
                     /locus_tag="PLD05_13700"
     CDS             complement(4425..5423)
                     /locus_tag="PLD05_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78543.1"
                     /translation="MWDRPQRFRRRPDRHLFACSLQVRAVVLSATALVMALASRGYPP
                     VFAQQVRLDVTNSSVSEGFFEYQGTSWTLMGPSGFARFGQSHTQVSPFPVFESPPPAL
                     GVHYGSRGWQGEFFGYWSSGIQRSHRSLTLSQTLSDGIYGSFACQSLRPFVIGFHPVF
                     ASAPYAPFAEPPALTYQHLPNPGPPMPNPRPAVAKDTQRAAARAPADRENDLPAEKLE
                     GSARAEDEPPLVLGGRPGPAEAADSAKGDRIGSLSQSSAERVVMSVDEARALRSAEQQ
                     RDNQEAQRYCDLAEAAMKRGEQGQARIYLRMAEKLASGDLRRKVLLLQQNLGEAEE"
     gene            5967..7505
                     /locus_tag="PLD05_13705"
     rRNA            5967..7505
                     /locus_tag="PLD05_13705"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1760 a         2076 c         2125 g         1544 t
ORIGIN      
        1 cctcgctgtt gtcccttcac aacctggaca ccatgcttac cgatttcaat tgacttgacc
       61 cgcgactctc cttgagcttc aatccgctgt cggttccata agatccatac ctccagatca
      121 aggagagaga gttccgcagg ctgaatcgcc ggaaagcgta gatcccatgt ggccgcgatg
      181 tgagccgcgc gttccaaggc ggtcgggagt aagacctcat cctcgatgtg cccacaacag
      241 gcccgaagct gttgcccact cttgagactg acgaatacac cccacaccgg gaccgccggc
      301 acggccgggg actggccctc ggaaatcggc ttgtcttggc caacaatcgc cgagagcacg
      361 cactcggccg cccgccggag gacgttttct ttgtcgtctt cagtcagttc cggccgggtg
      421 ggaacccaaa cactctttga tggcgaaccg ctgggagacg cgggatccga ttgtgacggg
      481 gttgtcgatt caaacatgac gttgactctc cttggtcagg gcgatccgcg ggagcatctg
      541 ggctagggca gggggggcca cggtgcactg acaaccctgc cggccgagct gctcctgccg
      601 gttgacgata gatttatcgc tggtaagttc cttatttcgc gaactccgaa atcctcacgg
      661 gatagcgacg cgcgccccac gtgcctggtg cgcctgctgt actgaaccgt ccggcaatct
      721 ccgacccaca gtaacgacat cggttccctt gtaaaccata tcggccaatc cagtaaccgt
      781 tccgttcgat caccagttgt ccacagtggg ggcagtaggt gctctgccga acggtatcga
      841 gcacgttccc tgtgtaaaca tagtgcaaac ctacgtcgcg ggcgatttca taggcctcga
      901 taagtttctc ggcaggggtg gggggacggt caaccaaccg aaaatcggga tgaaacgcgg
      961 taaagtgaag cggcacgtcg ggccccagct cgtccacaat ccagcgacac atctggcgaa
     1021 tttccgtggg atcatcattt tcttggggaa tgattagatt tgtgatttcg acccacacgg
     1081 aggtcttatt gacgaggtat ttcagcgttt ccaagactgg ttgcagcttg gcgctacata
     1141 attccttgta gaatctttcc gaaaaggcct tcaagtccac attggccgca tccatgtact
     1201 caaagaaata ctcgcgggcc ggagaggata tgtaacccgc ggtaacggcg acattcttaa
     1261 tgcccacctc tcgacacgcc ttggccgtat caacggcata ttcggcaaag atcacgggat
     1321 cgttataagt gtaagcgaca ctggtacagc caagggtctg agctgcgcgg gcaatcgcct
     1381 tgggcagagc gatttcactt aacctttcga tttcccgcga tttggagatt gaccaattct
     1441 gacagaactt gcaacctaaa ttgcacccgg ccgtcccaaa agagagtacc gccgtcccgg
     1501 ggtagaaatg gttcaaaggc ttcttctcga taggatcaat gcagaagcca gtactccgac
     1561 cataggtggt cagcaccatc tccccgttaa ggttttggcg aacaaaacag aagccgcgct
     1621 ggccttcgcc gagccgacag tttcgcggac acaaatcaca gacgatcttg ccgtcttctt
     1681 cgtgccacca gccggcggga catatcccgt cagcggttgc cgcggccaca ggtgggagtt
     1741 ccacatgacg agccatgatc aaacaactcc gtgcatatac cccgggttga tcggtttcaa
     1801 cggctgaacg cttacatttt cattttacgg ccagaacaga attagaccta agcctacgag
     1861 aaagcccggc aggaatccca ggcaaagacg caatcccaat cgggggtagg ccataattcc
     1921 acctaaaaga cttttcacgc ccatatttgc caatatggcc gcagcaatgt accgccagcc
     1981 gttgaggctt aagtcctgat cttggagtgc catgcggctg attgacagcg tgatggcgtc
     2041 catatcgtgg agcccggaga gaacggccag tggatagaga ccactagccc ccacatgctg
     2101 tctgatccaa gcaataagaa agagaacgat ggcatacaac gcagcgaaga acaatgcggc
     2161 gcgaagttgt gtgggattcc ggtgagacgg cacgacttga agttgctgat cacggacgcg
     2221 ccgccagagg aacggcacag ggaggtaagt tgccacagac atcaaggcga gcgggacaac
     2281 acaatggtgg agcattccag ggtggacaac aagaatctct acgaagaccc tcgcgaacat
     2341 caccgttgag gcaaggagga ttatgatgat tgtggaaact tcgtcctgac gattttcctg
     2401 ggattgtcga gctgcgctga tggttgtggc cgtacttgag accgccccac caaggattcc
     2461 cgccagaaga atagtggccc gctgtccgag gaatttgtaa gcgatgtacc cgctcaaact
     2521 aatgccaaca atcaaaacca ccatcatcca gacattgaag ggactgatca cccccagcgg
     2581 gtccacggtg tttcgaggca ggaccggcag gataatgcaa gtgataagga cgaactgcat
     2641 gatggcccga acatcttcat ctttgagccg ttcggagata cggtgaagct cgggtttgaa
     2701 ttgcaaaagt acggccaccg ctcctcccat cacgacgccg atttccaaag gtccaatcac
     2761 gaggagagct cctactccgt acatcagaat cacggccaca ctggtggtta gcccaggatc
     2821 gggttcagct cgctgaatcc ttagccaatg ggggaaaaac agtagtgcga cgagacaaac
     2881 cagtgcagcc gcgggtagcc agcctccaaa ccaggtgccc aagattgcga agacactccc
     2941 cgaaagggtg atgagaggga atgtcctcaa acccggcatc cccccttcgg tatgttccct
     3001 ttgcatacca acgaggaatc cgagaagcgc cgccattccc agacggccaa aaagcgctgg
     3061 catgctaagg ggggtggttt cgctaaataa gattccactt ggccaatccg gcatgggacg
     3121 tgactccaaa ggcgacccta aaagacaacg gcgactcaag aaaagtatgg cttacgaatt
     3181 actctcgccg gctcagatgg ccaatgctgg actgaaaata tttctgacgc cgccgttgga
     3241 tctcggcacg ttctccatcc gaggggacac cttgagcacg aagatcttcc agattagcca
     3301 agcagatagc gcaaccaacg gtctcgagat gaattttcac gtatgtcgac tccggctcag
     3361 ttagggcccc cagaagatac gccccgagag cctcccgcga ggggcaactc aagcggttcc
     3421 gacgccaaat ctcggccacc gagtgaatac cattatcccg ccgagatacg atgcgacgta
     3481 atcgcaataa acactcgtgg ttatgccgga gggcattttc aatcgccacc atccgctcag
     3541 ggggcagcgc ctcatccaaa taggcttcaa gctcttggtc cgaaattctt tcctgggcca
     3601 tcgttcgctt tcgttacttt cggcttcagg ttgactattt ttcttcccct tgaagttccg
     3661 gaaacacatc ttgaggaagc ccctgtgccc gtagtcgctc tcgtatgcgc cttaagaaat
     3721 cgaatttcca attggcaacc tgctgctcat cgatgtgcag atattgggcc gtttctttgt
     3781 tactccaccc gcacaagaac agcagttcca tacacgacac tttcaaccat tctccctggc
     3841 ttttccaagc catgatctgt ttttcgatgg tctcggaaag ggcctgttct tcgatgcctc
     3901 ggcgttcgtg actggccgcg accgtgctag ggccacgttg gggcctgcca ccaatggcac
     3961 aacgattcgc gccctcgatt ccctcctcta aggacagagt gatgcgccgt ccttgccgcc
     4021 ggtagtagtc cgtcaactta tgggccgcga tagaaaacag ccaattctcc aaagaccgcc
     4081 gactgtcgta gttagggagg ctcaccagaa acccgacaaa agtctcctga accaattcct
     4141 cagcgatatc tcggtgtttc acatgactcc caagatatga gtagagtcgg ccctcgtagc
     4201 ggcggataac ctctttccac gcctcgggat cgcccgaccg gacgcgagaa atcagatgtc
     4261 gttcactatc tgatgtttgt tccgccatgg tactggtgtt ttcttgtcgc ctgtcgagaa
     4321 aaacggataa gcggcggtaa cctgttaccc ctttgatgat cctggccgct aaccgtccag
     4381 aatacatgaa atgtacgggc aagtcttccc tggagtgggg ccgatcactc ctcggcctcg
     4441 ccaaggttct gctggagcag taagactttc ctacggaggt ctcccgaggc aagcttttcc
     4501 gccattcgta agtagattct cgcttgccct tgctcaccgc gcttcatagc cgcttccgcc
     4561 aaatcgcagt aacgttgtgc ttcttgattg tcccgctgtt gctcagcgga ccgaagcgcc
     4621 cgtgcttcat cgacactcat cacgacccgt tcggcggaac tctgagatag cgatccaatc
     4681 ctatcgccct tggccgaatc cgcggcttcg gccggacccg gccggccacc gagtaccaac
     4741 ggcggttcgt cttccgcccg agctgaccct tccagctttt ccgcaggaag atcgttctca
     4801 cggtctgctg gtgctcgcgc cgccgcgcgc tgagtatctt tggccacggc tggtcttggg
     4861 ttcggcatgg gcggcccagg gttgggcaag tgttggtacg ttagggcggg aggttcggca
     4921 aaaggagcgt aaggagctga tgcgaagacc ggatggaagc cgataacgaa cggtcttaac
     4981 gattggcatg cgaaggagcc gtaaataccg tccgaaagcg tttgcgacaa cgtcagtgaa
     5041 cggtggcttc gctggatgcc ggatgaccaa taaccaaaga actccccttg ccaccctcta
     5101 cttccgtaat gaaccccgag ggccggcgga ggactctcga acacggggaa gggcgaaacc
     5161 tgcgtgtgcg actgtccgaa ccgggcaaag ccactcggtc ccatcaaggt ccaacttgtc
     5221 ccctgatatt caaagaaccc ctcggagacc gaagagttgg taacgtcgag acgcacctgt
     5281 tgggcgaata ctggggggta tcctctagac gcaagggcca tgacaagtgc agtggcggac
     5341 agcacgaccg cccgcacttg caatgaacaa gcgaaaagat gtcgatcggg ccgacgccga
     5401 aatctttgag gtctatccca cataagccgt gaccctcttt tgaccaggcg taccccttga
     5461 cgagttttgg tccgtcgctc attattgtaa ttgtcaggca tgcgaaaggc tattgtctga
     5521 gccctgccga gcggtcaaaa gattttgcga gaggacctct agtcggggca agaatcggac
     5581 gataacataa aaagtgtccg aggcaataat ggccgaagga tgcctgaaaa ggctgccccg
     5641 cctagtaaag cccaccttaa gacggtaact taatccgtaa gatggggtgg aaaaaagggt
     5701 cggcaggagg cggaataccc ccctgttgac cgcatgaaag cgggtgctat aattcaaatt
     5761 gccgccgggg tacggtggca caaagtttaa gcgttgctct ttgacaattt ggtagcgtgc
     5821 ggttacaagc ccctaaacgc atctggtaaa ccggatgctt aaaaaaccac tccgggcgac
     5881 cgggagtggt cctgtgatca agcctttcat gtgttcgcaa caccttgtcc ggcacgataa
     5941 gtgttggaga aggtaagcgt acatatcaat tgaagggttt gatcctggct cagagtgaac
     6001 gttggcggcg tggattaggc atgcaagtcg tgcgacgagg cccgcaaggg tgctcgagcg
     6061 gcgaaaggga gagtaatgca cgggtaacct gcccctagga ctgggatagc cgtgggaaac
     6121 tgcgggtaat accagatgat gtctgatagc ttcggctatc ggaccaaagg tgcgagtccg
     6181 cctagggagg ggcccgtgtc ctatcagcta gttggtgggg taacggctca ccaaggctac
     6241 gacgggtacg gggtgtgaga gcatgtcccc gctcactggg actgagacac tgcccagaca
     6301 cctacgggtg gctgcagtcg agaatcttcg gcaatgggcg caagcctgac cgagcgacgc
     6361 cgcgtgcggg acgaaggccg ttgtgttgta aaccgctgtc aggggggagg aaatgtcggc
     6421 cctcagggtc ggcttgacct atccccagag gaaggacggg ctaagtccgt gccagcagcc
     6481 gcggtaagac ggaccgtccg aacgttactc ggaattactg ggcttaaagg gtgcgtaggc
     6541 ggctttgcaa gtcgggtgtg aaagccctcg gctcaaccga ggaactgcac tcgatactgc
     6601 gaggctcgag gaagatagag gtgagcggaa cttgcggtgg agcggtgaaa tgcgttgata
     6661 tcgcaaggaa caccggaggc gaaagcggct cactgggtct ttcctgacgc tgaggcacga
     6721 aagctagggg agcgaacggg attagatacc ccggtagtcc tagccctaaa cgatgagcac
     6781 tagaccgagg aactctccat ggtttcttgg tcgtagggaa accgttaagt gctccgcctg
     6841 gggagtatgg tcgcaaggct gaaactcaaa ggaattgacg ggggctcaca caagcggtgg
     6901 aggatgtggc ttaattcgag gctacgcgaa gaaccttatc ccggtcttga catgcacgga
     6961 ttaaccccgt gaaagcgggg ccacgcctct ctgaggtgga acgtgtacag gtgctgcatg
     7021 gctgtcgtca gctcgtgtcg tgagatgtcg ggttaagtcc cttaacgagc gaaacccctg
     7081 tcgctagttg ccagcgggtc atgccgggca ctctagcgag actgccggtg ttaaaccgga
     7141 ggaaggtggg gatgacgtca agtcctcatg gcccttatga ccggggctgc acacgtccta
     7201 caatggcgcg tacaaaggga agcaaacccg cgagggggag caaatcccaa aaagcgcgcc
     7261 ccagttcaga ttgcaggctg caacccgcct gcatgaagcc ggaatcgcta gtaatcgcgg
     7321 atcagcatgc cgcggtgaat gtgttcctga gccttgtaca caccgcccgt caagccacga
     7381 aagtggggga catccgaagt cgccgggcta acccgaaagg gaggcaggcg ccgaagatgg
     7441 actccgcgat tgggactaag tcgtaacaag gtagccgtag gggaacctgc ggctggatca
     7501 cctcc
//
LOCUS       DAOSUT010000188         7453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4931_length_7453_cov_8.575696, whole genome shotgun sequence.
ACCESSION   DAOSUT010000188 DAOSUT010000000
VERSION     DAOSUT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_7453_cov_8.575696"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(608..1018)
                     /locus_tag="PLD05_13710"
     CDS             complement(608..1018)
                     /locus_tag="PLD05_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HOP78544.1"
                     /translation="MPDLRILRGVKAFNAGRFFLAHEIWEDVWRDSSPPSKDYYQALI
                     QAAIAFYHLQRGNFVGARRLADRALGRLEHFDPQYLGLDTCRLRLSLEQILTGDLTGQ
                     KDQINDQTCPSCVPDRLDLPVFIGILDDTIGKLD"
     gene            1301..1975
                     /gene="nth"
                     /locus_tag="PLD05_13715"
     CDS             1301..1975
                     /gene="nth"
                     /locus_tag="PLD05_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOP78545.1"
                     /translation="MPRSPKTIVPKVLEVLKQEYPDASCSLNFSNPLELLVATILSAQ
                     CTDERVNQVTKDLFKKYKTAHDYASVPLEELEKDIQSTGFYKNKAKNLRECCRQIEER
                     FGGRVPKELDQLVSLPGVGRKTANVVIGTAYGIPSGVVVDTHVSRISQRLGLTQNTDP
                     EKIEQDLMKLIPQDEWIDFSHRMIKHGRKYCSARNPSCESCPMTAFCPKIGVETTGRK
                     KGSANR"
     gene            2145..2723
                     /gene="dcd"
                     /locus_tag="PLD05_13720"
     CDS             2145..2723
                     /gene="dcd"
                     /locus_tag="PLD05_13720"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOP78546.1"
                     /translation="MILSGKEILAQLGKNIIIEPFNPKNLNSNSYDLTLHNELMIYEE
                     VVLDMKKPNRVRRITIPEEGLVLTPHQLYLGRTVERTETYGFVPMIEGRSSVGRLGLF
                     VHVTAGFGDVGFRGYWTLEMFAIQPVRVYPGVSICQIFYHEIRGDYEEYRSDKYQNNQ
                     DIQPSLLFKELNPDACRRPKSHLPFGIEESTG"
     gene            2720..4498
                     /locus_tag="PLD05_13725"
     CDS             2720..4498
                     /locus_tag="PLD05_13725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOP78547.1"
                     /translation="MKSEKAAAASRLPAALSKQVTAEGKGRPSQPHNRFATFFGLAAL
                     CTVLTAIDVAPERGGPGVTCDEFYHISYGKRLVASFHANGLGSFRPSCIESTFAWRKD
                     GPPVHPPLGNWVLGWANWLFDPEPFNPLRVAITPARLGTAICFGCLALIVGLFCAAHF
                     GTISGVIAVVSLVCMPRLFGHAHLAALDLITATMCTATIVAVTWAEQHKRWYSLIVAG
                     VIWGLALLTRFHGLLCLPPVVCYLCYRYRWRAMPLAMLWLISGLITFFLGWPWLWLDP
                     LGHIQQYLISSTQRIHIHTFYLGRVWNDTVVPWHYPWVMFLVTLPPGLLFLGLAGIIS
                     PFLRARSISETQQKVSTTEPAGIWLVFGTICFFLLVFSLPGVPVYDGVRLFLPVYPLW
                     AILVGQGTLVASTYLKCTMQRHHTLRAILVCAFFASQAVGTVWFRPVWLSYYNCLVGG
                     LAGAEKLGFEVNYWGDALTEEILCRAAEQIRPGESLSFAPSLAPYQAAGILVASPCLM
                     KQEILLKAWSQETDDESTISRWLLIYHRRSDLPESLVRLIDERPAVAEVTRQGVWLAK
                     LIALGGADVTRPAVNGSDPTASSESP"
     gene            complement(4531..6036)
                     /locus_tag="PLD05_13730"
     CDS             complement(4531..6036)
                     /locus_tag="PLD05_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP78548.1"
                     /translation="MMNRTCRVWAIASVWLVVCGTVSNSIMAKETADLPNFVIIFMDD
                     MGYADIGPFGAEGYETPNIDRMAKEGRIFTDFYVTQAVCSASRAGLVTGCYNVRVSIF
                     GALNHRAQIGLHPDEVTIAEIVKQKGYATKCIGKWHLGHHPKFLPTNQGFDEYFGLPY
                     SNDMWPFHPTAGKNYPDLPLMENDRVIDENVTHEDQNMLTTWYTERAVEFIQRNKDRP
                     FLLYLAHSMVHVPLHVSDRFRGKSQRGLFGDVMMEVDWSVGQILDALRSNNLAEKTLV
                     IFTSDNGPWLSYGDHAGSAKPLREGKGTMFDGGCRVSCVMWQPGTVPAGTICREPAMT
                     IDILPTIAYRIGARLPDRKIDGQNIWPLIVGEPNAKSPHEALFFYWGSELQAVRMGRW
                     KLHFPHAYQTLGGRPGGRDGNPVPYEKAEIGLSLFDLETDPGETTNVIDQHPDVVARI
                     EKLADAMRQELGDSAKGLKPTAARPAGKLEDGDLRFHWEPGKPIDTVPSYP"
     gene            complement(6100..6450)
                     /locus_tag="PLD05_13735"
     CDS             complement(6100..6450)
                     /locus_tag="PLD05_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOP78549.1"
                     /translation="MFKDLASLASLLRNAQDLSERLNSVIAQLKNERLVGSAGAGLVT
                     VEVNGLAEVIRVSIDQRLIDPKDKDLLEELVAGAMRDAIQKARERHMALMTELTGGLP
                     IPGLDQVAKLFGGR"
BASE COUNT         1662 a         2048 c         1950 g         1793 t
ORIGIN      
        1 cgagcaagga tcgacctttc ctccccgcag gccgatagat ggattgacga aaagctaatt
       61 actaggacga acaaccacaa agtcacacgc ctgggtggcc atacctgctc taatattaac
      121 cgtccttttc cgacaattga aaaacgattc tgtgacgcgg gacggctcag agcatctttc
      181 atagggatca tggaataatt accctccctc cggttcgcgt cttatctacc tggggcaact
      241 ggcgcccgta atacattgtg ccatttcgcc acgaaaggat cgactttaac ttccggagat
      301 taccacaact ggcgttttaa cggctgcgag tattcacggt atgtcacctt tcactttcca
      361 acgttgacgc gaccattggc cttttgtgct tagagcctat cccatgatct cccctcgccc
      421 gttcggcggg agaggggtcg ggggtgaggg tggctcgaat cgctgccggt tttctcaatc
      481 aattccgacg ttcgcttgcc ctcaccccaa ccctctcccg gagggagagg ggggaccttt
      541 ttggggcagg gtcttagttt gggggtttct ttccgtctgc tatgtaaaag aacggggaat
      601 cttccctcta atcgagcttt ccaatggtat cgtccagtat gccgataaag acaggcaggt
      661 ccaggcggtc cggcacacac gaagggcatg tctggtcgtt tatctggtcc ttttggccgg
      721 tcaggtcgcc ggtcagaatc tgctccaaag acaatcgcaa ccggcaggta tcgagtccca
      781 aatactgagg atcgaagtgt tcaagacggc ccagggcacg gtctgctaac cggcgcgccc
      841 ccacaaaatt tcccctctga agatgataga acgctatcgc tgcctgaatc agggcttggt
      901 agtagtcttt ggatggtggc gaggaatctc gccaaacgtc ctcccaaatc tcatgggcta
      961 agaaaaaccg accagcatta aaggctttga caccgcgtag aatgcgaaga tcgggcaatg
     1021 ggtatctccg ttatacttgc ggacacggtc gatcgcattt cgccagaggt tcacaacttc
     1081 gctctgaatt ccattctacc aaatcgcttg ggggatgatt ggagaacaga aacacctttg
     1141 tgatttcatc cggaaatccg cctgtacctg atggctctct cctgggtgat tgacgtaccg
     1201 ttggaagacg caacaatgat tcctttcaga aggccttccc cggccgagca acggctcgca
     1261 atcaaaaaat ggctaccatc ataggcaaag gttcaaggtt atgcctcgtt cccccaagac
     1321 gattgttcct aaggttcttg aggtccttaa gcaagagtac cccgatgcgt cctgttccct
     1381 aaactttagc aatccgcttg agcttttggt ggcaacgatt ctatcggccc agtgcacaga
     1441 cgaacgggtc aatcaggtca cgaaagactt gttcaagaag tacaagacgg cccacgatta
     1501 tgccagcgtg ccactggaag aactcgaaaa ggatatccag agcacaggat tctacaagaa
     1561 caaggccaag aatctgcggg agtgctgccg ccaaatcgag gaacggttcg gtggccgggt
     1621 tcccaaagaa ctcgaccaac ttgtatcgtt gccgggtgtc gggcggaaga cggccaacgt
     1681 cgtgatcgga accgcgtatg gaattccgtc cggggttgtg gtggataccc atgtgagccg
     1741 aattagtcaa cggttagggc ttacgcagaa caccgatccg gaaaagattg agcaggatct
     1801 catgaaactg atcccacagg acgagtggat tgactttagt catcgcatga ttaagcacgg
     1861 aagaaagtac tgctcggccc gcaatccatc ttgcgaatcg tgccccatga cagcgttttg
     1921 ccccaagatc ggtgtggaga caacaggacg aaaaaaggga tcggcaaacc gctaagggct
     1981 acgcgaaaaa gggttgcctg gactgtgggg ccttctttct agcggggtcc gctatttatc
     2041 atagcgctta ttatgcttac gtgttgaaaa tagcgtcgtt ggtcgaacga aatcccatac
     2101 gctgatcaaa aactggccat tggcagcaca agcaaggaga cagcatgatc ctttcaggca
     2161 aggaaattct cgcccagttg gggaaaaata tcattatcga gccctttaac ccgaaaaatc
     2221 tcaactcaaa cagctatgac ctgacgctcc acaatgagtt aatgatttac gaggaagtcg
     2281 tcttagacat gaagaaaccc aatcgggtac ggcggatcac catccctgag gaaggccttg
     2341 tattgacccc ccatcagctt tacttgggca ggacagtcga acgaactgag acgtatggct
     2401 ttgtaccgat gattgaaggc cggtcctcgg ttgggcgtct tgggctattt gtgcatgtca
     2461 ccgcgggttt tggagacgtg ggattccgtg ggtattggac gttggagatg tttgctatcc
     2521 agcctgttcg ggtctatccc ggcgtatcca tttgtcagat tttctatcac gagattcgcg
     2581 gcgattacga ggagtatcgc agcgacaagt accaaaacaa ccaggacatt cagccgagcc
     2641 ttctctttaa ggaattgaat cccgacgcgt gtcgccgacc caaatctcat ttgccgttcg
     2701 gtattgagga atcgaccgga tgaagtccga aaaggctgct gccgcgagta ggttaccggc
     2761 agcgttaagt aaacaggtaa cagctgaggg taaagggaga ccatcgcaac cccacaatcg
     2821 cttcgcaacg ttttttggcc tcgcggctct ttgcaccgtc ctcacggcga ttgatgtcgc
     2881 tcctgagcgc ggtggtcctg gcgttacttg cgatgagttc tatcacattt cgtatggcaa
     2941 gcgattggtg gcctcctttc atgctaacgg cctaggttct tttcggccat catgtattga
     3001 aagtaccttt gcatggcgta aagatggccc acccgttcat ccgcccttag gcaactgggt
     3061 gctgggctgg gcaaactggc tttttgaccc tgagcccttc aatcccttac gagtcgccat
     3121 tacgcccgca cgattgggca cagcgatctg ttttgggtgt ttggccctca tcgtgggtct
     3181 tttttgcgca gcacacttcg gcacaatcag cggcgtcatt gccgtggtct cgctagtatg
     3241 catgccccgg ctgtttggtc atgctcacct tgccgccctc gatttaatca cggccaccat
     3301 gtgcaccgcg acgattgtgg ccgtcacttg ggccgagcag cacaaacggt ggtacagttt
     3361 gatagtagct ggcgttattt ggggacttgc cctacttacg cgattccacg gactattgtg
     3421 cctaccgccg gtggtctgct atctttgcta tcgctatcgg tggcgagcga tgcccctggc
     3481 gatgctttgg ctgatctcgg gccttatcac tttctttctc ggctggccgt ggttatggct
     3541 tgatcctctc ggccacatcc agcagtacct catcagctcg acgcaacgaa ttcacatcca
     3601 caccttttac ctcggccggg tctggaacga cacggtcgtg ccgtggcact atccctgggt
     3661 gatgtttctc gtcacgttac caccgggttt acttttcctc gggctcgctg ggataatatc
     3721 gcccttttta agagcgcggt ccatttccga aacccagcag aaggtgtcca ccactgaacc
     3781 tgccggtatt tggctggtat ttgggacgat ttgcttcttc ttgctggtgt tttctctacc
     3841 cggggtcccg gtttatgacg gggttcgcct gtttttgccc gtgtatccgc tgtgggcaat
     3901 cctggtaggc cagggaacgt tggtagcctc gacatatctg aaatgtacga tgcagagaca
     3961 ccacacgctt cgagcgatcc ttgtatgcgc gttttttgcc tcccaggcgg tcggcacagt
     4021 ctggtttcga cctgtctggt tgagttatta caactgcctc gtcggagggt tagcgggggc
     4081 cgaaaaactt ggcttcgagg ttaactactg gggtgatgcc ttaacggaag agatactttg
     4141 ccgtgcggcg gaacagatcc gaccgggcga gtcgcttagc ttcgcgccga gccttgctcc
     4201 ttatcaagca gcaggaatcc tggtcgcatc cccgtgcttg atgaagcagg aaatccttct
     4261 caaggcatgg tcgcaagaaa cggatgacga gtcaactatc tcccgttggc tccttatata
     4321 ccaccggcgt agcgacttgc cagagagtct cgtccggctt attgatgaac gtcccgcggt
     4381 agccgaagtc acccgacagg gcgtttggct cgccaaactg atagcactcg gcggggcaga
     4441 cgtaactcgc cccgcggtta acggctccga tcccacagcc tcctcagaga gtccctgagg
     4501 acgccggagg tggtttacct cagcggtcag ttagggatag ctcggcacgg tgtcgatcgg
     4561 ctttcccggt tcccaatgaa accgcaaatc gccatcttcc agtttgcccg ccggtcgtgc
     4621 cgcggtgggt ttcaagcctt tggctgagtc acccaattcc tggcgcatgg catccgcaag
     4681 tttctcgatg cgggcgacca catcaggatg ttggtcgatg acattcgtcg tttcgccggg
     4741 atcggtttcc aggtcaaaca gcgacaagcc gatttcggcc ttttcgtacg gtacgggatt
     4801 cccgtctctt ccgccgggtc gcccccccaa ggtctgataa gcatggggaa aatggagctt
     4861 ccagcgaccc atgcggacgg cttgaagctc tgatccccag tagaagaaca gggcttcatg
     4921 cggcgacttc gcattcggct ctccgacgat caaaggccag atattttgac catcaatctt
     4981 gcgatcaggc aatcgcgccc cgatgcgata ggcgattgtg ggaaggatgt cgattgtcat
     5041 cgccggctct cgacaaatgg ttcccgccgg cactgtcccg ggttgccaca tgacgcagga
     5101 gacccggcag ccgccatcga acattgtccc cttcccttcc cggagtggtt tcgccgaccc
     5161 ggcatgatca ccatagctca gccaagggcc attatcagat gtgaagatga caagcgtctt
     5221 ctcggcgagg ttgttacttc ttaacgcatc aaggatctgt ccgaccgacc agtcaacttc
     5281 catcatgaca tcgccaaaca gtccgcgttg actcttgccg cgaaagcgat cagacacatg
     5341 gaggggaacg tgaaccatac tatgtgccaa ataaagtagg aacggtcgat ccttgttccg
     5401 ctggatgaac tcaaccgccc gctccgtgta ccaggttgtt agcatattct gatcctcgtg
     5461 ggtgacgttc tcatcgatca cccggtcatt ttccattaac ggcagatcgg gataattctt
     5521 ccccgctgtt gggtgaaatg gccacatgtc gtttgaatat ggcagcccaa aatactcgtc
     5581 gaatccctgg tttgttggga gaaacttggg atggtgtccc aggtgccatt tcccaataca
     5641 cttggtcgca taacccttct gcttgacgat ttctgcaatc gttacctcgt caggatggag
     5701 accaatctgg gcacgatggt tcaacgcccc gaaaatactc acacgaacgt tgtaacatcc
     5761 tgtgacaagg cctgcccgcg aagccgaaca cacagcctgc gttacgtaaa aatccgtgaa
     5821 gatccgtccc tctttggcca tgcggtcgat gtttggagtc tcgtaccctt ccgctccaaa
     5881 cggaccaata tccgcgtagc ccatatcatc catgaaaatg atcacaaaat tcggcagatc
     5941 agcggtttcc ttcgccatga ttgagttgct aaccgttccg caaaccacca gccagactga
     6001 tgcgatcgcc caaacgcggc atgttctgtt catcatagcc tccctcatca agatggaccc
     6061 cacaacgaaa acaacactcc tccacaacgg gacccaattc tagcggccac cgaaaagttt
     6121 ggccacctgg tcaagtcctg gaataggcaa tccacccgtc aattccgtca tcaaggccat
     6181 atgacgctcc cgggccttct ggatcgcgtc ccgcatggca ccggcaacca gttcctcaag
     6241 caaatcttta tccttgggat cgatcaatcg ctggtcgatc gaaacgcgga tcacttctgc
     6301 caggccgttc acctcaaccg taaccaaccc ggcccccgca cttccgacga gcctttcgtt
     6361 ctttaattga gcgatcaccg agttcaatcg ctccgaaagg tcctgcgcat ttcgcagcaa
     6421 cgaggccaac gatgccagat ccttgaacac agacaacctt gcactccttt cctaccgata
     6481 ggtttcgcac aactgagtca tgttagtcct ttcaatgttg ttacccattc tagtcgctca
     6541 cgcactctgg gaaaataaag accggtggct ctccattgtt gagttcgcgt caacgtcggc
     6601 cgacggcgga acccttccaa aacgttgacc gatcttaagc cctttagagc ttatcccaaa
     6661 aagcctcccc ctctccctcc gggagcgagt tggggtgagg gcaaacgaac gacggaattt
     6721 attaagaaat cgggcagcga ttcaagtcac cctcaccccg acccctcctc cgccgaacgg
     6781 gtcagggaga ttatgggata gactcttgag gtcgcaattt cggatagatc ggcggagcct
     6841 cgtgattgtg cgttggattt cgaagtaccc gccaggagtg cggacggttg caaaggccgc
     6901 ttcgtgcgac gtgtacctct aacagagtgc tgacagtgga tttagagaat ccacgtaaaa
     6961 ttttccggat aactgggctt ttgcatggta gtggcagccc aaaggatcca gatgtcacaa
     7021 cctcttttgg cgacaagact tgggaaaaaa ctcagttttt ccggcgatgg tggcttgcaa
     7081 tccattgggc cgcaggtcat aatgagccta aatcgcggtg aggagcgatt ggcgcttggg
     7141 gtattgacgg atcttgcagg ctcctgtaaa atcccttagg tacgttgctc gtttctcccg
     7201 accttttgag tttgcggtgg ttaaaggggc acgagtaaca acagtattgg cgttatctca
     7261 aggcggttgt gtcgatagat tgtccctcca cgggttttcc gccttgtctt gattgggcgt
     7321 aacttgggtt gacgatagat ccccggtgac ggtttagccc catttaggga aagccgtggg
     7381 ggattggggt gccaaccaac taaacgccca acacgctgag aaaaacctga ttcggctacc
     7441 gcacgtagtc gat
//
LOCUS       DAOSUT010000189         7388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_4989_length_7388_cov_9.639711, whole genome shotgun sequence.
ACCESSION   DAOSUT010000189 DAOSUT010000000
VERSION     DAOSUT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_7388_cov_9.639711"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLD05_13740"
     CDS             <1..271
                     /locus_tag="PLD05_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78550.1"
                     /translation="SSQMDTIPCLEELKPGDRVEVEHQVTVGGKSWTTRTVGTVVRTE
                     RVCHGLHFERAPDERTYSDMILLELPDGELTAVTVDEFTVIRRAP"
     gene            446..>1087
                     /locus_tag="PLD05_13745"
     CDS             446..>1087
                     /locus_tag="PLD05_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOP78551.1"
                     /translation="MSDRASVFLQHSRNFGEFRFVYPVVSRRARGVSVGVNLNPDKVC
                     NFDCVYCQVDRRSPGNKAFVEMEQLRAELLAAIQLVQTGALFEHPTFAATPLPLRRLN
                     DIAFSGDGEPTTFANFDAVIQLAADVRRAESLDDVKLVLITNASMFHREVVQRGLAIL
                     DENNGEIWAKLDAGTPEFFAKVARTRIPFERILENITQAAQIRPIVIQSLFMRW"
     assembly_gap    1088..1187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1370..2395
                     /locus_tag="PLD05_13750"
     CDS             1370..2395
                     /locus_tag="PLD05_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOP78552.1"
                     /translation="MRPTSKGFRWFLYSAGVLLLLLQTEPLLAQKWAEAMFDHTSHNF
                     GMVARGAKVEHVFPLKNIYVEDVHIASVRTSCMCTQPTITKETLKTFETGAIVATVDT
                     RGFLGRKDATITVTFDKPYPAEVQLHTYVYIRSDVVFEPGAVLFNAIPQGTSAHRTVQ
                     LSYAGRNDWQIVRAMPSADFLDVEVNETGRGAGEVRYQLVVTLKPQAPPGYLQEHIEL
                     FTNDTVADAQKIVLAVEGSIVPQVSIQPSQLALGSVTPGQVVTRNLVIQARKPFRVVS
                     IKGPDERFSFVLPETTKSVQVIPVIFKAGDRAEPIKGEIQIATDLAGFETLRVSVFGT
                     FAGTDAQ"
     gene            2477..3235
                     /gene="kdsB"
                     /locus_tag="PLD05_13755"
     CDS             2477..3235
                     /gene="kdsB"
                     /locus_tag="PLD05_13755"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOP78553.1"
                     /translation="MKSWIVIPARLASTRLPRKMLLSETGKPLIQHTYEAASQARRPA
                     GICVACDDEEIARVVRSFGGDARLTSPHAQSGTDRVAEIARSMPDVEIFVNVQGDEPE
                     ISGEAVDLAVELLERHPQASVSTVAAPLRNREALHDPACVKVVCDKDGYALYFSRSVI
                     PYPREWSEEYLTASPPCFLQHMGLYAYRRSFLLSVSNLEVPYLERLEKLEQLRFLYHG
                     AKIVVGLVDSPTCGIDTPEDYRRFVERYRARYTR"
     gene            3377..5032
                     /locus_tag="PLD05_13760"
     CDS             3377..5032
                     /locus_tag="PLD05_13760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOP78554.1"
                     /translation="MAKHIFVTGGVVSSLGKGLTSASIGMLLEHRGLSVRMQKMDPYI
                     NVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTNSPLTKDSNYTTGKIYLSVITKE
                     RRGEYLGQTVQVIPHITNEIKRAITALGGPGVDVVITEIGGTVGDIESLPFLEAIRQL
                     SLDLGRENCLYIHLTLVPYLRAAGEIKTKPTQHSVGQLRQIGIQPDILICRTEKPLGR
                     DARAKIALFCNVPVECVIEERDKDFSIYEVPISLVENNLDELIVRKLGLKAGPLNLEK
                     WYDLLHRIRNPKAEVSIAVVGKYVEHRDAYKSIYEALDHAGIAHHAQVRVERVHSEKV
                     EQEGPERLLAGVDGILVPGGFGERGIEGKVEAIRFARERGIPFFGICLGMQCAVIEFA
                     RHVAGLKHAHSTEFHKDTPDPVVCLLDEQRTKTDKGGTMRLGAYPAVLQPNSLAAKAY
                     GTLEISERHRHRYEFNNEYRHILTEKGLAITGTSPDGKLVEIIELPQHPWFVAVQFHP
                     EFKSQPTKAHPLFKAFIESALKTRAQRASSQFSQSAQSSALAD"
     gene            5213..6451
                     /locus_tag="PLD05_13765"
     CDS             5213..6451
                     /locus_tag="PLD05_13765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOP78555.1"
                     /translation="MAEHCRFDHAPVRILHLFSRLNCGGAEKRTLEVARHIQRQPELR
                     QRIQIDYCVLSGQPGVLDEEARALGGRIHYLPLRQASFPRRFTALLRREGYAVVHSHV
                     HLASGILLELARLAGVPHRIVHFRSQSDGKSYLLRGPLRWLLKRLIDHAAVRILSVSR
                     CVMEAVWGEDWSRDERCQIIYNGIDVDGRSVIHDQGYKEKLVQSLGLPPNSRLMVHVG
                     SYRAPKNHPRLIDIFRQVHRQESRSHLLLVGDLSQGLSQLKSRLASYRLGNVVHFLGL
                     QKDVAHLLRAADVMIFPSSWEGLPGAVLESLAVGTPVVCSDIPPHREIAQFLPGLSCV
                     PLSAPDEHWAKIICSLAPPTVEDREKACQAFMESPFSLDRCVASLVAIWEEGPTARRV
                     PPSGLEEPSERWNVDTGRAA"
     gene            6448..>7388
                     /locus_tag="PLD05_13770"
     CDS             6448..>7388
                     /locus_tag="PLD05_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78556.1"
                     /translation="MKLAQFSLILAVIAAGFAIALKRLGDYWGVEFPEITHFLLYAPA
                     IGFGLVSWLARPSVLSHWSIVLWAAMPVLGMFYGESPSLLTSGVIAFYLLSGPALAAL
                     IDESRSWLRVARWAVLVNSLLVFAIFYLTYRCYDGSLYHAFVKFGYIPLRDGSFGANP
                     NQLGGQLAFASVLGLAIFFRSGRAPEKEGRVTPLFIQGRLPCGAFSAHRESVSRILPP
                     AAGGVVPAKSARPVRRFTFVKADWLVILASLVTAGGCLMTGSRGAVLSLIIGAFVVIG
                     GSAGLQPLHRLRDFAVIVGMSLLMGTLATTVLPVNPV"
BASE COUNT         1481 a         1972 c         2130 g         1705 t
ORIGIN      
        1 cagcagccag atggacacaa ttccgtgttt ggaggagctc aaaccaggtg accgcgtgga
       61 ggtcgagcac caggtaaccg tcggcggtaa aagctggacg acgcggacgg tgggaaccgt
      121 ggtacgaacc gagcgggtgt gccacggtct gcacttcgag cgggcacccg atgagcgaac
      181 gtacagcgat atgatcctcc tggaattgcc cgacggcgaa ttgacggccg tgacggtgga
      241 tgagtttacc gttattcgca gggcaccttg aggagggctc tgatcctgtg ctgacatgga
      301 atctcggggc ggagggttgg atttctagct ggcctgttgg gacgggcgat cttccgtaat
      361 ggacgaggtg ggcagtcgcg atggggtttc ctcgccttcg ttgttgatct gtcttgttga
      421 atttctttgc taaagggaat ctgcgttgag cgatcgggcc tcagtttttt tgcaacacag
      481 ccgaaacttt ggcgagtttc gctttgttta tccggtcgtc agccgccgcg ctcggggtgt
      541 gtcggtgggt gtcaatctca atcccgacaa ggtgtgtaac ttcgattgcg tctattgcca
      601 agtcgatcgc cgaagccccg ggaacaaggc ctttgtggaa atggagcaac tgagagccga
      661 gcttctggcg gccatccagt tggttcagac gggggcgctc tttgaacatc cgacatttgc
      721 agctaccccc ctgccccttc gacggctcaa cgacatcgct ttcagcggtg atggggaacc
      781 aacaacattc gccaatttcg atgccgtgat tcaacttgcg gcggacgtcc gtcgtgccga
      841 gtcactggac gatgtgaaac ttgtcctcat cacgaatgcc tccatgtttc accgagaggt
      901 agtccaacga ggtttggcca ttctcgacga aaacaacggc gagatctggg caaagctgga
      961 tgccggcacg ccggagtttt ttgccaaggt tgcgcggacg cggattccct ttgagcgaat
     1021 tctcgaaaat atcacccagg ctgcccagat tcggccgatc gtcatccaat cgctgttcat
     1081 gaggtggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca ttgccaagcg
     1201 tagtaaaaga cgttattctt ttgccgttaa agataaccca acttgcgcca accggtcagt
     1261 tttctgagat tccggtccga acgatctacg agccctcctt gcggcagcac accgctaaca
     1321 acgagcaggg atggtgatgc atccggcgtg tttagggagt ttcatcacga tgagacccac
     1381 atcgaaagga tttcggtggt ttctttactc ggctggtgta ttgctattac ttctgcaaac
     1441 agaaccgtta ctggcccaga agtgggcgga ggcgatgttc gatcatacga gccacaactt
     1501 cgggatggtc gcccggggtg ccaaagttga gcatgttttc cctttgaaaa acatttatgt
     1561 cgaggatgtc cacatcgcct ccgttcggac atcctgcatg tgcacgcaac ccacaattac
     1621 taaggagaca cttaagacct ttgaaacggg ggccattgtc gccacggtgg acacgcgagg
     1681 gtttctggga cgcaaggatg ccaccatcac ggtgactttt gataagcctt atccggcgga
     1741 agtccagttg catacgtacg tttacatccg cagcgacgtg gtctttgaac ctggggccgt
     1801 gctcttcaat gcgatacccc aaggcacgag cgcgcacagg acggtccagc tcagctacgc
     1861 gggacgaaac gactggcaaa tcgtgcgggc aatgccaagc gcggattttc tggacgtaga
     1921 agtcaatgaa acgggtcgtg gggccggtga ggtccgctat cagcttgtgg tgacgctcaa
     1981 gccgcaggcc ccgccgggct atctccaaga gcacattgag ctgtttacga acgatactgt
     2041 ggccgatgcc caaaagatcg tcctggccgt ggaaggttcc atcgtgcccc aggtctcgat
     2101 tcagccttcg caactcgcat tgggtagcgt cacgcccggt caggttgtca cccggaatct
     2161 ggtgattcaa gcccgcaagc cgtttcgggt tgtgtccatt aaggggcccg atgaacgctt
     2221 ttcgtttgtc ctgcccgaaa cgaccaagag cgtccaagtc attccggtca tttttaaggc
     2281 aggtgaccgc gcggaaccca tcaagggaga gatccagatc gcgaccgatt tggcgggttt
     2341 cgagacgctt cgggtctccg tttttgggac gtttgccggc accgatgcgc aatgacaaat
     2401 agactcgttc ggtacgttgg ggctggctta ctcttccggc actgatgatc gctagttgag
     2461 caccaggtga atgtcaatga aaagttggat tgtcatccct gcgcgtttgg cttccacgcg
     2521 tttgccgcga aagatgcttc tttccgagac gggcaaaccg ctcattcagc acacgtatga
     2581 ggcagcctcc caagcccgac gaccggccgg aatctgtgtg gcttgcgatg atgaggagat
     2641 tgcccgcgtc gttcgctcct tcggtggcga cgcccgcttg acaagtcccc acgcccaaag
     2701 cgggacggac cgcgtggcag agattgctcg gtctatgccc gacgtggaga tatttgtcaa
     2761 cgttcaaggc gatgaacccg agatttccgg cgaggcggtt gatctggcgg tggaactcct
     2821 ggaaaggcat ccccaggcca gcgtttccac agtggccgca cctttgcgga atcgggaagc
     2881 attgcatgat ccggcctgcg taaaggttgt gtgcgacaag gacggctacg ccctatattt
     2941 cagccgtagt gtcattccgt atccgaggga atggagcgag gaatatctca ccgcctcgcc
     3001 gccgtgtttt ttgcagcaca tgggattgta cgcctatcga cggtcgttcc tcctcagtgt
     3061 ctcgaatctc gaagttccct atttggagcg tctcgaaaaa ctggaacagc tccgcttctt
     3121 gtaccacggg gccaagatcg tggtgggcct ggttgattcc cccacctgcg ggatagatac
     3181 ccccgaagac taccgacggt ttgtcgagcg ataccgggcg cggtacactc ggtagagagg
     3241 ttgagtctct aggtggcgtc tgcgcggttg tcatggcagg ccctgcggac gcaccgtctg
     3301 atccttgccg atgttcggcc gcatcaaaaa acctgaggat gcggttcttt ttgttttatg
     3361 agcgagattc gcacaaatgg ccaagcacat ttttgtcacc ggtggtgtgg tcagttcctt
     3421 gggcaaaggg ctgacgagtg catcgatcgg gatgcttttg gagcatcggg gactttccgt
     3481 gcggatgcag aagatggacc cgtacatcaa cgtggatcca gggacaatga gtccctatca
     3541 gcacggggag gtttacgttc tcgatgacgg cagcgagacc gatctcgacc tcggccatta
     3601 cgagcgcttt accaacagcc ctctgacgaa ggatagcaac tacacgacgg gcaagattta
     3661 tctttctgtc atcacgaaag aacgtcgtgg cgagtatctg gggcagacgg ttcaagtcat
     3721 tccgcacatt acaaacgaga ttaagcgggc catcacggcc ctgggtggac cgggcgtcga
     3781 tgtcgtcatc acagagattg gcgggaccgt gggcgacatt gaaagcctcc cctttttgga
     3841 agccatccgc cagttatccc tcgacttagg ccgcgagaat tgcctgtata tccatttgac
     3901 cttggtcccc tatcttcgcg cagccggaga gattaagacc aagcccacgc aacattcggt
     3961 cggacagctt cgccagatcg gtatccagcc ggacatcctc atttgtcgga ccgaaaagcc
     4021 cttgggccgg gatgcccgtg ctaagatcgc cctcttctgc aatgtccccg tggagtgcgt
     4081 cattgaagaa cgcgataagg atttctctat ttatgaggtc cccattagcc ttgtggagaa
     4141 caatctggat gaactaatcg tgcggaagct cggcctgaaa gcgggaccat taaacctgga
     4201 gaaatggtac gacctactcc atcgtatccg gaaccccaag gcagaggtct ccatcgccgt
     4261 ggtgggaaaa tacgtcgagc accgcgatgc ctacaagtcg atctacgagg ccctcgatca
     4321 cgcagggatc gcccatcatg cccaggtgag ggtcgaacgt gtgcacagtg aaaaggtcga
     4381 acaggaaggg cccgaacggt tactggccgg cgtcgatggt atcctggttc ccgggggatt
     4441 cggtgagcgc ggcatcgagg ggaaggtgga ggccattcgc tttgcccgag agcgaggcat
     4501 tccctttttc ggcatttgcc tggggatgca gtgtgcggtc atagagttcg cccgccatgt
     4561 ggccggtctc aagcacgccc attctactga gttccacaaa gatacgcctg accctgtcgt
     4621 ttgccttttg gatgaacagc ggacaaaaac cgacaaaggc ggcacgatgc gtctgggggc
     4681 ttatccggca gtgcttcagc cgaacagtct cgccgccaag gcctacggca cgctggaaat
     4741 cagcgagcgg catcgacatc ggtatgagtt taacaacgag taccgacaca tcctcaccga
     4801 aaaagggctg gccattacgg ggacgagccc ggatggaaag ttggtggaaa ttatcgaact
     4861 gccccagcac ccgtggttcg tcgccgttca gttccatccc gaattcaaat cgcaaccgac
     4921 caaggcccat cctttattca aggcatttat cgaatcggcc ctaaagacac gggcccagcg
     4981 ggcttcttcg caattttcac aatcggcaca aagctcagct ttggccgatt gaggtcgctc
     5041 gtccaatgca cctgcgcaaa atgattaaaa gtcattcggc cgttcgcact ggtctaaatt
     5101 cgccttgaag ggaatgttgc ctttgtgata cccttcggca ttcgcatgcc aacgatcatc
     5161 acgattgccc acggctcttg accgggccgg tcatcatgag aagcggtttc tcatggctga
     5221 acattgccga tttgatcatg caccggtgcg gatcctgcat ctcttttccc gcctgaattg
     5281 tgggggtgca gaaaaacgga ccctggaagt ggcccgtcac atccagcgcc agcctgaatt
     5341 gaggcagcgg attcaaattg actactgcgt cctttctggc cagccgggag tattggacga
     5401 agaagcccgc gcgctgggcg gtcgcattca ctaccttccc cttcgccagg ctagctttcc
     5461 gcgacgattc acggccctgt tgcgacgcga agggtacgcg gtcgtgcact cccacgtgca
     5521 ccttgcttcc gggattcttc tcgaacttgc ccgccttgcc ggagtccctc atcgtattgt
     5581 ccatttccga agccagtcgg atggcaagag ctaccttttg cgggggcccc ttcggtggtt
     5641 actaaaacgg ttgatcgacc acgctgctgt acggattctc agtgtcagcc gctgcgtcat
     5701 ggaggccgtg tggggggaag actggtcccg tgatgagcgc tgccagatta tctacaacgg
     5761 gattgatgtc gatggacgat cggtaattca tgaccaggga tacaaggaaa aactcgtgca
     5821 gtcgctggga ttgccgccaa acagccggtt aatggttcat gtggggagtt atcgtgcccc
     5881 caagaaccac cctcggttga tcgatatttt tcgccaagtt cacaggcagg aatcccgatc
     5941 ccacctgctg ctggtagggg acctctcgca gggattgagt caactgaaga gtcggctggc
     6001 gagttacagg ctggggaatg tcgttcattt cctcggcctg caaaaggatg ttgcccatct
     6061 gttgcgggcg gcggatgtca tgatctttcc ctcatcatgg gaggggctac ccggcgcggt
     6121 cctcgaatca ttggccgtgg gaaccccggt cgtctgctct gatatcccgc cgcatcgcga
     6181 gattgcccaa tttttgccgg gattgagctg cgttccatta tcggcacctg acgaacactg
     6241 ggcaaagatc atctgctcgc ttgctcctcc gacggtggaa gaccgcgaaa aggcctgcca
     6301 ggcattcatg gaaagtccct tttcgctgga tcgttgcgtg gcatcgctgg tagccatctg
     6361 ggaggaagga ccaaccgcca ggcgtgttcc accatcgggg ctagaagaac cgagcgagcg
     6421 atggaatgtc gatacgggga gggccgcatg aagttggcgc aattctcgct gatcctcgcc
     6481 gtgattgcag ctggctttgc gattgcgctc aagcgattgg gagattattg gggcgttgag
     6541 tttcccgaaa ttacccattt tctcctttac gccccagcca ttggcttcgg tttggtcagt
     6601 tggctggctc ggccgagtgt cctttcccac tggtccattg tgctttgggc ggcaatgccc
     6661 gtgcttggga tgttctacgg agaatctcca tcattactca caagcggggt gatagcgttt
     6721 tatctcttga gtggtcctgc tctcgcggct ttgattgacg agtcccgaag ctggttacgg
     6781 gtggcgcgct gggcggttct tgtcaatagt cttcttgttt tcgcaatatt ttacctaact
     6841 taccgctgtt acgacggctc gttgtatcat gccttcgtca aatttggtta tataccgctt
     6901 agggatggaa gcttcggggc caatccgaat caacttgggg gacaactggc ctttgccagt
     6961 gtacttgggc tggcaatatt ctttcggtcc gggcgtgccc cagaaaagga ggggcgagtc
     7021 actcctcttt tcatccaagg gcgtttgcct tgcggggcat tttcagccca tcgagagtca
     7081 gtgtcccgga tcttaccacc tgcggcaggc ggggtggttc cggctaagag tgcgcgaccg
     7141 gtacgccggt ttacattcgt gaaggccgac tggcttgtga tcttggccag cctggtcacg
     7201 gcgggcggat gtcttatgac gggttcccgg ggtgctgtcc tgagcttgat tatcggggcg
     7261 tttgtcgtca tcggtggaag tgctggcctt cagccccttc acaggttgcg ggattttgcc
     7321 gtcattgttg ggatgagcct tctcatgggc actttggcca cgaccgtcct gcctgtaaac
     7381 cccgtcat
//
LOCUS       DAOSUT010000190         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5023_length_7333_cov_10.594119, whole genome shotgun sequence.
ACCESSION   DAOSUT010000190 DAOSUT010000000
VERSION     DAOSUT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5023_length_7333_cov_10.594119"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1007..2428
                     /locus_tag="PLD05_13775"
     CDS             1007..2428
                     /locus_tag="PLD05_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOP78557.1"
                     /translation="MWTSAQRPATLKELRESGWRSKTVKEELRDNFIRALQSGEDLFP
                     GIIGYDDTVIPEISLAILAGHDMLFLGEKGQAKSRLMRLLTRFLDPAIPYLDIPDVPV
                     HEDPYRPITRRAKEFLAETPEDKVPIAWWPREQRYAERLAPGTKFADIIGEIDPAKLA
                     RGTSLSAEEALFFGLIPRMHRGIFAVNELPELDELVQVGLFNILEERDVQIRGYPIRF
                     DLDILILFSANPATYNRSGKVIPQLKDRIGSVIHTHYPRDRDIAIQITEQEAKIDLNG
                     AYPVVVPYFMKEIIEQISIVARRSKYVDQQSGVSARLSIANYRTMVASARRRGIILGE
                     KPAVPRISDLGHLASSSLGKLELDLMAGHQMTEKQVLDAIIAEAIKDVFQEYVQEHGL
                     AEIAEIFGRGVKIEVGSMLPSAYYAERLKRVPPAWEKAFEINVSNDPAVRASCVEFIL
                     AGLYVTDRISRAEYRGKIVYEIS"
     gene            2415..4115
                     /locus_tag="PLD05_13780"
     CDS             2415..4115
                     /locus_tag="PLD05_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78558.1"
                     /translation="MKSPKHLGGVIHVYQKYNPLRFPSPTKPPPDLASAAVNHMLTFG
                     SLRELSEEELAQAIELDPSMIAGLGPSLESLMEMLRQRKAKILATYEVETVHALAARR
                     YRELGSSLKPPQKLSRRYERAFREEQIFELERLWYLCETENSEFARKVLHLVQALGDK
                     YLIDELAAKYHFTGQRPMTIPEALEIKDILETIDRLLEQLQEASKHARLAVIDLEALR
                     RFVEEYDIERLKGFMRQIEDLIRQIAEEQGLERTAQGYQLTPKAYRLFQGRLLEKIFS
                     QLQAARSGRHTGPVIGDGNVELPQTKAYEFGDSLAHMDIPGTMINAMIRQGPGIPVYL
                     HPDDIVVHKTRNVPKCATVALLDMSGSMRYGGLYVDVKRMGLALDGLIRKEYPGDYLE
                     FVEIYTFARRVHPSEIPSLMPKSVTLYDPVVRLRADMSDPRITEMDIPWHFTNIQRGL
                     QVARQLLANQDTPNRQIILITDGLPTAHFEDRYLYLMYPPHDRTEEATLREGLLCARE
                     GIVINIFLLPGWTQSREDVRFAYKLAESTRGRVFFTAGKDLDRYVVWDYLERRREIIG
                     "
     gene            4236..5306
                     /locus_tag="PLD05_13785"
     CDS             4236..5306
                     /locus_tag="PLD05_13785"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOP78559.1"
                     /translation="MPRVDRWMIYGAYGFTGSWIAREAVRRGLRPILAGRNASRLVPL
                     ADELNTEYRTFSLDEPNVVREALQDCRLVLNCAGPFSQTAQPMVMACLETGTAYLDIT
                     GEVDVLEWIAQLHESAVRRNVTLIPGLGFDVVPTDTLAVLLKEAFPSANLLQLALTGT
                     GSLSVGTTRTIIEACQKGGRIRRNGQIVPVPLGWKKRRVPFRPRRLWAMTVPWGDVAT
                     AWYSTGIPNIEVYLAMPIWAIIAVRLFFPRRVSARGWPYLQHLEPLLRRIFGTSPPGK
                     TAQKVDLWGCVSNDQGNTKTATMQTPDGYQLTVETTLAAVSAMLDQEYPGGFFTPTQA
                     FGINFALQIDGVDFRFEDDTQR"
     gene            complement(5332..6237)
                     /locus_tag="PLD05_13790"
     CDS             complement(5332..6237)
                     /locus_tag="PLD05_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOP78560.1"
                     /translation="MSEAAHWRLLTEPGHPEVWFGNAEIVSNDQVARDTFCLRMAAAP
                     IAGVITPGQFMMVRLPQTHDPLLGRAFALYDVVRNDKDQAIGLEIAYAAVGRMTRRLS
                     TLRPGEKLEIWGPLGNGFPAPFADELILVAGGIGYTPFLAVAKEALGVTQYGKTARTG
                     GRCCVVKLLFGVRSKDYVPSDIYRFSESGVQVVLSSDDGSVGKRGFVSDLLDEELSVP
                     VRGRRVVYCCGPEPMMAKVAEICRERSVECLVSLETPMACGLGLCYSCMARIKVNAQD
                     AGWDYRRVCVEGPVFDARRVVFEDS"
     gene            complement(6319..7092)
                     /locus_tag="PLD05_13795"
     CDS             complement(6319..7092)
                     /locus_tag="PLD05_13795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOP78561.1"
                     /translation="MAAQRKPQQEPTEPRGERLQKVLAAAGIGSRRQCEELIRMGRVE
                     VDKKTITELGFRVDPRRQKICVDGVPIKLEPHVYYAVYKPDGVVCTNRDPAGRTRVID
                     LVPETNLRLFPVGRLDLHSEGLIIVTNDGTLAERLTHPRYGVPKTYIVQVAGEPTREI
                     FTKLLRGVHLAEGVARVAKIRVRRKQRKSTILEMVLNEGRNREIRRIMARVGHKVYRL
                     KRIAVGPIRLGKMKPGEARRLTAAEVKALYQVAGLRQDS"
BASE COUNT         1662 a         2073 c         1928 g         1670 t
ORIGIN      
        1 ctggagatgt ggtgccccga aaggggttgc gggttcgagt cccgtgccct ccgctcggta
       61 gtctgtttag aacccatccc aaaaagtccc cctctccctc ggggagaggg ttggggtgag
      121 ggcaagcgaa cgtcggaatc gattgagaaa accggcagcg attcgagtca gcttcgcccc
      181 gggccccttt cccgccgaac gggcgaaggg agattactgg ataggttctt ggttcgccac
      241 aacggtagat ttgggcctgc gatcacggtt gcgacgcttc tgctgttaac cccgtgtcgt
      301 taggtatatc ggcggcccgg ttgaattggg atcaaaaccg ctgatcactc tcgaaactca
      361 gctctgacaa gtccttcttt tcggaagccg acccagggaa ttccagtggt agccagtgtc
      421 tccgaaacga gaccaatttc gtacaccacg cccggcgttg attccatccg gtgacggcct
      481 cttcgctctg tactaggtct tctggtaccc cttgtgaaat caaatcctat ctgtcacggt
      541 ttttttcgat ttacctaatg tgcattgacc tcgcgacccg cgcttgacgg tgtggtcctc
      601 ctcgggggca tgtcctcccg tacaacgtag tgacgagggc cgcggagctt ttggctcata
      661 ttgtgcttgc gcgcgatgga tgtcgttgcc ctttgttaaa attcgctccc cctttttcga
      721 cgattgtgca ccgcacaagt cttccattca cgtctgcgtt gccacaaacc atattcatcg
      781 cgtggtcact agttgtccat tccgacatgc cagccggcaa attcagccgc agcaacggtc
      841 gagggtatcg tgcgcttggt ggatcaactg gggtggaggt gcagtatggg tgcacttaac
      901 ccattgccat tccgcgctac aatgaaagcg ggtcaacatc ccaggggtga ttaagaaatc
      961 ttgtgtgcga gacagctgcc cgcgctgaag aaggagcgga acaaccatgt ggacctcggc
     1021 ccaaaggcca gcaacgctga aagaacttcg ggaaagcgga tggcggtcga agacagttaa
     1081 ggaggaattg cgcgacaatt ttatccgggc gcttcaatcg ggcgaggatc tgtttcccgg
     1141 catcatcggc tatgacgata ccgtcattcc ggagatcagt ctcgccattc ttgcgggtca
     1201 cgatatgctg tttctgggtg aaaaaggcca agccaaaagc cgtctcatgc ggttgcttac
     1261 caggtttctg gaccccgcca tcccgtatct tgatatcccc gacgtgcctg ttcacgaaga
     1321 cccgtatcgt ccgatcacgc gccgagcaaa agagttcctg gccgagacac cggaagacaa
     1381 ggtccccatt gcttggtggc cccgggagca gcgctatgcg gagcgactgg caccggggac
     1441 aaaatttgca gacatcattg gcgaaatcga ccctgccaaa ctggccaggg gcaccagtct
     1501 ttcagccgag gaggccttgt tctttggatt aatcccgagg atgcatcgcg ggatcttcgc
     1561 cgtcaatgag ttacctgaat tggatgagct agttcaggtg ggccttttca atatcctgga
     1621 agagcgggac gtacagattc gtgggtatcc catccggttt gatctggata ttctcatcct
     1681 gttttccgcc aatcctgcta cttacaaccg aagtggtaag gttattccgc agttgaaaga
     1741 ccgcatcggt tctgtgatcc atacgcatta tccgcgtgac cgcgacattg ccatccaaat
     1801 caccgaacag gaggctaaga tcgatctgaa cggcgcgtac cccgtggtgg tcccttattt
     1861 catgaaagag attatcgagc agatcagtat tgtcgcgcgg cgatcgaaat atgtggacca
     1921 gcaatcgggc gtcagtgccc ggttgagcat tgccaattac cggaccatgg tggcgagcgc
     1981 ccgccgtcgt ggcatcatct tgggtgaaaa accagctgtc cctcgcatta gtgatcttgg
     2041 tcacttggcc tcgtcctcgc tgggaaaact ggaactagac ctgatggcgg gccaccagat
     2101 gaccgaaaaa caagttttag acgctatcat tgcggaagcg atcaaggatg tctttcaaga
     2161 gtacgtgcag gagcatggcc ttgccgagat cgcggagatt tttgggcgag gcgtgaaaat
     2221 tgaagtgggc agcatgttac cctcggcata ttacgcggag cggctgaaac gggtaccccc
     2281 cgcctgggaa aaggcttttg aaattaacgt ctcgaacgat cccgccgtga gggcttcctg
     2341 cgttgagttc attttggcgg gattatatgt gaccgaccgc atttcgcggg ccgaatatcg
     2401 tggaaagatc gtctatgaaa tctcctaagc atctaggcgg tgtcattcac gtttatcaaa
     2461 agtacaaccc gttgcggttt cctagcccca cgaaaccgcc gccggattta gcgtcggccg
     2521 ccgtcaatca catgcttacg tttggttcgc tgcgggaatt gagcgaggaa gaactcgctc
     2581 aggccatcga gcttgaccca agtatgatcg ccgggctagg cccttcgctg gaatcgttaa
     2641 tggaaatgct ccgccagcgt aaggccaaga ttcttgcgac ttacgaagtt gaaactgttc
     2701 atgccttggc agcccgtcgt taccgagagt tgggaagtag cctaaagcca ccgcagaaac
     2761 tatctcgccg ctacgaacgg gcgtttcgcg aagagcagat ctttgaatta gagagacttt
     2821 ggtacctatg tgaaaccgag aacagtgaat tcgcacgaaa agtgcttcat ttggttcagg
     2881 cactcgggga caagtacctt attgatgaac tggcggccaa atatcatttc acggggcaac
     2941 ggcccatgac aatccccgag gccctagaga tcaaagatat tctggagacc atcgaccgcc
     3001 ttcttgaaca gcttcaggaa gcctccaagc atgcgcgctt ggccgtcatt gatctggagg
     3061 ccctgcggcg gtttgtggaa gagtacgaca tcgaacgttt gaagggtttc atgaggcaaa
     3121 tcgaagatct cattcggcaa atcgccgagg aacaaggtct ggaacgaact gcccagggct
     3181 accaactgac gccgaaggcg taccgattgt ttcaagggcg actcttggag aagattttca
     3241 gccaacttca ggccgcccgc agcggccgac acacaggtcc tgtgatcgga gacggtaatg
     3301 tcgaattacc ccaaaccaaa gcgtacgagt ttggcgactc actggcccac atggacattc
     3361 ccggaacaat gatcaacgcc atgatccgac aggggccagg tatccccgtt tatcttcatc
     3421 cagacgacat tgttgtccac aagacccgca atgttcccaa atgtgccaca gtcgccctgt
     3481 tggacatgag tggctcaatg cggtacggcg gcctatatgt ggatgttaag cgaatggggt
     3541 tggctttgga tggactgatt cgaaaggagt atccggggga ttaccttgag ttcgttgaaa
     3601 tctacacgtt tgcacgtcgc gtccatccca gcgaaatccc atcacttatg ccgaaatcgg
     3661 ttacgttata tgaccccgtc gttcggttgc gtgccgacat gagtgatcct cgcatcacgg
     3721 aaatggatat cccctggcac tttacgaata ttcaacgtgg actccaagtg gcacggcagc
     3781 ttcttgcaaa tcaggacacc ccaaatcggc agatcattct catcaccgac ggattaccca
     3841 ctgcccactt tgaggatcgc tatttgtatc tcatgtatcc acctcatgac cggacagaag
     3901 aggcgacact tcgggagggt ctgctgtgtg cccgcgaggg gattgtaatc aacatctttc
     3961 tcttgccagg atggacacaa tcccgtgagg atgttcggtt tgcctacaaa ctggctgagt
     4021 cgactcgtgg ccgtgtcttc ttcaccgccg gaaaagacct cgaccgttat gttgtgtggg
     4081 actatctgga acgacgccga gagattatcg gctaaccgct tctcgataat tccggacgcc
     4141 gcttcgctac cgctccgtat ttgagataca ttggacgaag gcaacggttt ctcacggcgg
     4201 ccctcactgg atgtcaaaac taatggtgaa taactatgcc tcgggttgat cggtggatga
     4261 tctatggtgc atacggattc acagggtcct ggattgcgcg ggaggccgtc cgtcgtggtc
     4321 ttcggcccat cctcgccggt cgaaacgcct ctcgtcttgt tcctctagcg gacgaactga
     4381 acactgagta tcggacgttc tcgttggacg aacccaacgt tgtcagggag gctctgcaag
     4441 attgccgcct agttttgaat tgtgcgggcc ccttttccca aactgctcag cccatggtaa
     4501 tggcgtgtct cgaaacagga acggcctatc tcgatatcac cggcgaggtg gacgtgctgg
     4561 agtggattgc ccaactccac gaatcggcag tccgccggaa tgtcacattg atcccaggtc
     4621 tggggttcga cgttgtccct accgatacat tggcagtact gctgaaagag gcattcccgt
     4681 cagccaacct cttgcaactt gccttaacag ggactggctc actcagcgtt gggaccaccc
     4741 gcaccatcat cgaagcctgt cagaaaggtg gtcggatccg ccgcaacggg cagatcgttc
     4801 cggtcccgtt gggctggaaa aagcgacgtg ttccatttcg gccccggcga ctttgggcca
     4861 tgacagtccc ctggggtgat gtggcgaccg catggtattc cacgggaatc ccaaacattg
     4921 aggtctatct ggccatgcca atctgggcca tcatcgctgt ccgattgttt ttcccccgcc
     4981 gggtcagtgc acgaggttgg ccttatttgc agcatttgga accactgctg cgccggatct
     5041 tcggaacgtc accgcccggg aagaccgctc aaaaggtgga tttatggggc tgcgtaagca
     5101 acgaccaagg aaatacgaaa acggcaacga tgcaaacacc tgatggttat caactcacgg
     5161 ttgagaccac gcttgccgct gtctcagcga tgctcgatca ggaatatccg ggaggctttt
     5221 tcacgcccac acaagcattt gggataaact ttgccctgca gattgatggt gtcgattttc
     5281 gttttgagga tgacacacaa cgataggctt ttttgcaatt cgcctgtatc ttcacgagtc
     5341 ttcgaagaca actcgtcgcg catcaaagac cggcccttca acgcacacgc ggcgataatc
     5401 ccacccggca tcctgcgcgt tgaccttgat ccgagccatg cagctataac aaagtcctaa
     5461 cccgcatgcc atgggcgttt caagagaaac taagcactcc acgcttcttt cgcggcaaat
     5521 ctcggctacc ttggccatca tgggctccgg accacaacaa taaacgaccc ttcgccccct
     5581 gaccggaaca ctcaattcct catcgagcaa atcactgaca aaaccgcgct tgccaacgga
     5641 accatcatca ctgctaagaa cgacctgcac accggactct gaaaaacgat aaatgtcgct
     5701 cggtacataa tccttgcttc gcaccccgaa aagcagtttc acgacacaac aacgtccacc
     5761 agttcgggca gtcttcccgt attgggtaac ccccagggcc tccttggcta cggccaagaa
     5821 aggcgtatag ccaatgccgc ccgctaccaa gatcagctca tccgcgaacg gagcgggaaa
     5881 accattaccc agcggtcccc agatttccag cttctcgccc ggtcgcaagg tggacaaacg
     5941 acgagtcatt cggccaaccg ctgcatatgc gatttccaaa ccgatagctt gatccttgtc
     6001 attgcgcact acgtcgtaca gagcgaatgc ccgtcctaag agcggatcat gcgtttgggg
     6061 taacctcacc atcatgaatt gaccaggcgt gataactccc gcgatcggcg cggctgccat
     6121 tcgcaagcag aaggtatccc gagccacttg gtcgttggaa acaatttcgg cgtttccgaa
     6181 ccaaacttcg ggatgacccg gctccgtcaa caatcgccaa tgggctgctt cgctcattac
     6241 ctcaaacgct ttctttacat cgggcacgca cgacctagaa aaaggacact gtcgggccgg
     6301 ccttgtccag cctttccctc acgagtcctg gcgcagacca gccacctgat agagtgcctt
     6361 cacctccgcc gccgtaagtc gccgcgcctc tcccggcttc atcttcccaa gacgaatggg
     6421 ccccactgca attcgcttca gacgatatac cttatgtccc actcgcgcca tgatccgccg
     6481 aatttcccgg ttcctgcctt cgttcaagac catctcaagg attgtgctct tcctttgttt
     6541 tcgccgaacg cggattttgg caacgcgggc aactccctct gccaggtgga caccccgcaa
     6601 gagcttagtg aagatctccc gcgtaggctc tccggccacc tgaacgatgt acgttttcgg
     6661 aactccgtaa cgtggatgcg tcagcctttc ggccaatgtg ccgtcattag tgacgataat
     6721 caggccttcg ctatgaaggt ccaatcgccc cacggggaat agccggaggt tcgtttcagg
     6781 aacaagatca atgacccgcg tccggcctgc ggggtcacga ttcgtgcaaa ccacaccatc
     6841 cggcttatat acggcataat aaacatgtgg ctccaactta atcgggacac catcgacaca
     6901 gattttctgt cgtcgcgggt ccacgcggaa acccaattcc gtgatggtct tcttatcgac
     6961 ctcgacgcgc cccatgcgaa tcaattcttc gcactgccgg cgactgccaa taccggccgc
     7021 cgccaggacc ttctgaagtc gctcgccccg gggttctgtc ggttcttgtt gaggtttacg
     7081 ttgcgccgcc atgctattgg ccttatttgt tacgcggagg aaaaacagcc ttgaatccca
     7141 caagggagaa tgccacacgg ccatctcggc acgtgcaagg tgctggcacc agggaaagcc
     7201 tattttaaga aaatgagtcg tgtgccatcc catcattgta gaggcattca aacagatagc
     7261 tatcccagga tgctgacggg ctgtcgagaa taaccaagat aagaacacaa cctaccgact
     7321 cgccccggca gat
//
LOCUS       DAOSUT010000191         7205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5118_length_7205_cov_8.633007, whole genome shotgun sequence.
ACCESSION   DAOSUT010000191 DAOSUT010000000
VERSION     DAOSUT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_7205_cov_8.633007"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            644..>1805
                     /locus_tag="PLD05_13800"
     CDS             644..>1805
                     /locus_tag="PLD05_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOP78562.1"
                     /translation="MNEHVIRAYDALANQPVEMVLHEGQLRDCRPIDSTNETTTPADL
                     PYVAPGFFDLQVNGYQGRDFTSPQLTVDDVYAVADALWQFGTTRFLPTVITQAHEVLI
                     HCVRTIAQAVNEFPILKQRIPGIHLEGPYISTQDGPRGAHPREHVRPPDWEEFCRLQE
                     AATGLIRLVTLSPEYEMSLPFIEKASKEGITVAIGHTAASPEQIRSAVAAGARLSTHL
                     GNGCHLMVPRHPNYLWEQLAADDLYASFIADGFHLPAAFLKVALRAKRDRAVLVSDLS
                     AMAGLPPGRYSTPLCELEILETGQLVVAGQRALLAGAAEPITTGIVNAMVLGGATLRQ
                     AVAMATVTPCEVLKMEPPQFLAGTPSNLVVFEIEQENEQFASSDKPDLFPARK"
     assembly_gap    1806..1905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1906..2204)
                     /locus_tag="PLD05_13805"
     CDS             complement(<1906..2204)
                     /locus_tag="PLD05_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78563.1"
                     /translation="MHPEQRRAILVTRRVREAAGYVELGLFDRALECLEIEDLGPWEG
                     PVAMFKGQILAAQGRFHDAAAAFERAAQLFPPPHDRLAWLTLSQCLRRAGDTTRA"
     gene            complement(2492..4711)
                     /locus_tag="PLD05_13810"
     CDS             complement(2492..4711)
                     /locus_tag="PLD05_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78564.1"
                     /translation="MPINGPLRSGGGSLLRYANLRWIWGFIRIYGWILFFLSGINAAG
                     EGFTLLVNRTSQPIAIEMIAAQQDWLTPQQGAALPPVLGPGNMILFRSTPNVIARLRT
                     DPQKVFDVALSPNVVVIVDSLPQDERSGSYALTVTKMGRDDDLASAILELPKIHVSKG
                     YRCMRWIPGEVGHRIPVKLLVDDEEAATPAVWQARLVRRIKDVNEILMAFCGVYLEIV
                     SFDSWESDDRLSDIEDGFREFVAKVDPHPASLAIGFSAQWSAKSLSGPLGMSVGPLTS
                     HILVREYGAGITESERIEILLHELGHYLGAAHVPDDGSVMRSRLEKRPSRDRSFAIGF
                     DPINTLVINTAVDELRAGRRRVADFSPGACESLRAWYDVISRADPEDNTAVHLAALLP
                     SRPAESSLSTVERPSPSGNQADTARPESGATQPADESQSLPTTKRDVTLAEDRTEPQQ
                     TPKPPEKLPSSAPVSPSLHESKGQAVVESPPKQSAASGHVPAPEERPNLVAADVPQGA
                     RIILQQIVQSWSPPRPEDIPRGLPVGDYILEGLVRRAAEVAIRLDVDEEAKRRGAFLL
                     AIAVVSDDSNFVRNQPGLGEILQQIESDDERKLRLSRIGIATIFGRHDWAQHFCVSAG
                     LVCLLGAGPTRSLGLAKEWRDSQGDSGWSFADLGADFAGIRFGEAILKGEIPLEDVRA
                     GFQIADFVPPLADYPEDIPFTRFVATYGGLFGQPTRTILAEIDKAIQALPGYQRANR"
     gene            complement(4840..5040)
                     /gene="rpsU"
                     /locus_tag="PLD05_13815"
     CDS             complement(4840..5040)
                     /gene="rpsU"
                     /locus_tag="PLD05_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOP78565.1"
                     /translation="MVKLILREGESIQEAVRRFRKLVERSGIKREMRRREYYEKPSEI
                     RRRARLRAERRARRARLMMMGR"
     gene            5644..5877
                     /locus_tag="PLD05_13820"
     CDS             5644..5877
                     /locus_tag="PLD05_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78566.1"
                     /translation="MASKALSGVSDQMAQHLEIDEQLALIRDLESRHEDLFRKLEELE
                     RRVAEVLTQWAKPLSRGDASSSLAEQVAPQSKN"
     gene            complement(5967..>7205)
                     /locus_tag="PLD05_13825"
     CDS             complement(5967..>7205)
                     /locus_tag="PLD05_13825"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOP78567.1"
                     /translation="KVQHPDIEGKLLADLEILSGLAFLAERIPDFQIYRPMSVVAELR
                     RTLVREVDFVRELRNLQEFRAHFEDSPDVRIPRAFPELSTKRLLVLEYLDGCKLTEQD
                     RLKELGIDFDEVARRGAQAYLKMIFEDGLYHADPHPGNIMVFPDETIGLLDFGMVGRL
                     DDRLRERVQELLTALVSQDSERLAIIVAEVGATPISLDRAALTLDLADFVAYYGHMPI
                     EQFDLAGALTEMIEILRRYRIVLPARIALLLKTLIVLEGTAQLLSPRFHLLEVIRQYR
                     RKVLWHVFSPRIALRRAQRTYWELQSLLEVLPQALRDVVQQLRNGTFDIHLDHRGLEP
                     SVNRLVVGLMTSALFLGSALMLAHRVPPLLSDLPLLGGWLSENMGSISAPGFVGISLS
                     AIWGWRLWRAIRRSGRLDRH"
BASE COUNT         1671 a         2114 c         1851 g         1469 t
ORIGIN      
        1 agaacgaccg cggaaacaaa cttgctcatc atttcctccc ccacaacaaa agacggaatc
       61 gcatggaacg ccgacagttc tttttgagaa ctgggccaca ctggctggcc ctttcccatt
      121 cgatttggtg ggccgcgaga ggcaggtccc tatattcctc gccgtaagcc ccacagaacc
      181 cggtcagcct gcactcaata ttgcacctgt gtcgaacaac cctagctagt gtgggcaaaa
      241 gctttgtgac agtcaagcat gcaacaactt ggtttatctc gagcttcggc cttcgcaggc
      301 gacgtttgaa gcaccggagc gcgttcgtcg tcccataccg aagacatcgg cccattcgct
      361 ggccaagtct tccacatcga aaagctgaga gcgatcatcg cttcgataac ggtgctctct
      421 ggaaactgtg gcctggatag tgttttccgc tcaagcttca gatccatcaa cgatcttgag
      481 ctatcgcggt cttttccaga ggcacccaaa agcgggcgta ggcacgtcct ggtacgattg
      541 agggagttaa accacatcgt caagtgagta agatgggtga aacgactcgt ttgccccgga
      601 agagatggcc gccaggattc gcgttcaatg aaggacaacc cccatgaacg aacatgtcat
      661 tcgcgcttac gatgcacttg cgaatcagcc cgtcgagatg gtgctccacg agggtcaact
      721 ccgtgattgt cggcccattg actcgaccaa cgagactacg acccccgcgg acttgcccta
      781 tgttgcgccc ggctttttcg acctgcaagt gaatggttac cagggacggg atttcacatc
      841 accccagctt accgttgatg atgtctatgc tgtggcggat gccttatggc aatttggcac
      901 gacgcggttc ttgccgacgg tcataaccca agcccatgag gtcctgattc attgcgtgcg
      961 gacaatcgcc caggcggtca atgagttccc cattctcaaa cagcgcattc cgggcattca
     1021 tttggaaggg ccttacatca gcacccagga tggcccacgc ggtgcccatc cccgagaaca
     1081 cgttcgaccg ccggactggg aagagttttg tcgcttgcaa gaggcggcaa cggggctaat
     1141 ccggctagta acactatcgc ccgagtatga gatgtctctt ccctttatcg aaaaggcatc
     1201 taaagagggt atcaccgtag ccatcggcca tacggcggct agtccggagc agattcgcag
     1261 cgccgttgcc gccggtgccc gactgagcac ccacttgggc aacggctgtc atttaatggt
     1321 gccgcgccat cccaactatt tgtgggaaca attggccgcg gatgatttgt acgccagttt
     1381 cattgccgat ggttttcacc tcccggccgc attcctgaaa gttgctctac gagccaagcg
     1441 ggatcgcgca gttctggtaa gcgacctttc agccatggca ggattacctc ctgggcgata
     1501 ttccaccccg ctttgtgaac tagaaatctt ggagacgggt caactggtgg ttgccggcca
     1561 acgggcactg ctcgccggcg ctgccgagcc cattaccacc ggaatcgtca atgccatggt
     1621 cctaggcggg gcgacgttac gacaagcggt ggctatggcg accgtcactc cctgcgaggt
     1681 gctcaaaatg gagcctcccc aattcctcgc gggcacgccc agtaacctgg tggtctttga
     1741 gatcgaacag gagaatgaac agttcgccag ctcggataag cccgaccttt ttccggcgcg
     1801 caaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatagc tcgggtcgtg
     1921 tcaccggccc ttcggagaca ttgacttaag gtcagccaag ccagacgatc gtggggtggg
     1981 gggaatagct gcgctgcccg ctcaaatgcc gccgccgcat cgtggaaccg cccctgggca
     2041 gccaagatct gccctttgaa catggcaaca ggcccctccc atggccccag gtcttcaatc
     2101 tcgaggcatt ccagggcacg gtcaaacaga cccagttcca cgtacccagc ggcttcgcga
     2161 accctacgtg tcactaagat ggctcgacgt tgctcgggat gcatttaaca acctccgtac
     2221 ccgaccatag gggggagcac tgcgatgcaa caccggatca agccttaatc tgaacaaagt
     2281 aaaggagcaa cctacatgcc aaaaatcacc aaaaacacat aatacgtaag taatttccag
     2341 ataatgacat acgtctgcgt aaagtctttg accaagttcc attctcccca ttttgacaca
     2401 ctcggcggac agagacgtgc caatctgaca cacactcggc ccacatcata agggggatca
     2461 cactaccaga tggacgggtt caaagaacga gtcatcgatt tgctcgctgg tagcccggca
     2521 aggcctgaat agctttgtcg atctccgcca ggatcgtccg agttggctgt ccaaatagac
     2581 caccgtaagt agctacgaat ctcgtgaacg ggatatcctc cggatagtcc gctagagggg
     2641 gaacaaaatc cgcaatctga aagccggcac ggacgtcttc gaggggaatt tcccctttca
     2701 agatggcctc accaaaacgg atgcctgcaa aatcggcccc aagatcggcg aagctccagc
     2761 ctgagtctcc ctgcgaatct cgccactcct tggccaaacc caggctcctc gtcggccccg
     2821 ccccgagaag gcagactagc cctgccgaga cacaaaaatg ttgtgcccag tcgtggcgac
     2881 caaaaatcgt ggcaatcccg attcgcgata gtcgcagctt tcgttcgtca tcggactcga
     2941 tttgctgaag gatttcccct aagcccggtt gattccggac aaaattggag tcgtcactga
     3001 ccacggcaat cgccaagaga aaagcacccc gacgtttcgc ttcttcatcc acgtcgagcc
     3061 ggatggctac ctccgcggcg cggcgcacca gtccttccaa gatgtagtca cctaccggta
     3121 ggccgcgcgg tatgtcctca ggtcttggtg gcgaccagct ttggacaatc tgctggagaa
     3181 tgatcctcgc gccttgtggg acatccgcag ccacgaggtt cggtctctcc tcaggcgccg
     3241 gaacatgtcc ggaagccgcc gattgcttcg gtggactttc gacaacagct tgccctttgg
     3301 attcatgtaa cgaaggcgat accggggccg atgacggcag cttctccggt ggctttggtg
     3361 tttgctgcgg ctccgtccgg tcctccgcca aagtaacgtc ccgcttggta gtcgggagag
     3421 attgggactc atcggctggt tgcgtcgcgc cagactccgg ccgcgctgtg tcagcttggt
     3481 ttccactcgg tgagggtctt tccactgtgg aaagtgagga ttcggcgggc cgcgacggca
     3541 gcaatgccgc cagatgcacg gcagtattgt cttcaggatc agcgcggctg ataacatcgt
     3601 accaggcacg aagcgattca caggccccag gagaaaagtc ggccacacgc cttcggccgg
     3661 cccgcaactc atctaccgct gtgttgataa caagggtatt aatgggatca aaaccgatgg
     3721 caaaagaacg atcgcgcgaa ggccgtttct ccaagcgact tcgcatgaca gaaccatcgt
     3781 ccggcacatg ggcggccccc aaataatgcc ccaattcgtg aagcaggatt tcaatccgct
     3841 cgctctccgt gataccggca ccatattccc ggacaagaat gtgactcgtt aacgggccaa
     3901 ctgacatgcc gagcgggcca gacagcgact tcgcagacca ttgtgctgaa aagccaatgg
     3961 ccagcgaggc cggatgcgga tcgaccttcg caacaaactc tcgaaatccg tcctcgatat
     4021 cgctcaaacg atcatctgat tcccaggaat cgaaggagac aatctcaagg tacacaccac
     4081 aaaaggccat caatatttcg ttgacatcct tgatccgtcg gacaaggcgt gcctgccaca
     4141 cggccggggt agcggcctcc tcgtcatcca ccaagagctt caccgggata cgatggccca
     4201 cctctccggg aatccatcgc atgcatcggt aacctttgga aacgtgaatt tttggcagtt
     4261 ccaagatcgc ggaagccaag tcatcgtctc ggcccatctt tgttaccgtt agagcgtacg
     4321 agcccgatcg ctcgtcctgc ggcaacgagt ccacgatcac cacgacgttg ggcgaaagcg
     4381 cgacatcgaa caccttttga ggatcggtgc gaaggcgagc tattacgttc ggtgtggagc
     4441 gaaaaaggat catattcccg gggccaagca cgggtggcaa agccgcccct tgctggggag
     4501 ttaaccagtc ttgttgggcg gcaatcatct caatggcaat cggctgggat gtgcgattga
     4561 cgaggagggt gaacccttcg ccggcagcgt tgatcccgga gaggaaaaaa agtatccagc
     4621 cgtagatcct tatgaaaccc caaatccaac gcaggttggc atagcgcaaa agcgatccgc
     4681 cccccgaacg gagcggtcca ttgatcggca tggggccatg catgggatcg gaaagatcag
     4741 attaattatc ccgtgaggtc aaatacggag aacaacgatg atccgccaac ctgcggaagg
     4801 caatttgttc gatgacctag tagttgaata actatgcggt tatcgcccca tcatcatcaa
     4861 ccgtgcgcgc cgtgcccgtc gctcagcccg aagtcgtgcc cgtcgccgga tttcactggg
     4921 cttttcgtag tattctcgcc gccgcatttc ccgtttgata ccgctccgtt cgactagctt
     4981 cctgaaacgg cgaaccgcct cctgaatcga ctcgccttca cgcaggatca atttgaccac
     5041 ccggtaacct cctttccttt ctctaggaaa tgacttgcca accaaacgac aaaaacccaa
     5101 acctacttaa aaacccttcg ctacaacagt tctcgcctgc cccactaggg attgccccta
     5161 ataaatttta tggatcgacc gactagtccc cgcttcttga cctggcaaaa ctttcgggaa
     5221 agtcacttcg cgggaaatac tcggcaaccg cagtccagac cggccatgag ccaaacaact
     5281 ccggccaagc caacggcctc ctcgagtctc atcggcaaag tctgatcgta acggcttttt
     5341 atcctcctgt ccagcgggtg cgcattgcta atcgggggga tattctaaag ggagatacct
     5401 cgggctgacg aaaaacggtc ccccaaaaat cggacacggg actatccgca catccttccc
     5461 atctttgcta tattattcct ccttcgcaat cgtcgcagga cgaaaatctg gctttaccca
     5521 aatggttcgg ccatgtgctg tccgaaactt attcccagtc ctcccaacct cacggcgaca
     5581 aagcgtaacc gacagcgttg attcctttgc cgttttcctg gctacgcacg agcaaccgaa
     5641 gatatggctt ccaaagctct cagcggcgtt tctgaccaaa tggcgcagca cctcgaaatt
     5701 gatgagcagc ttgccctgat ccgcgatctt gaaagtcgcc acgaagacct ctttcgaaaa
     5761 ctggaagaac tcgaacgtcg ggtggcggag gtcctcacgc aatgggctaa accgctttcc
     5821 aggggcgatg cgtccagttc attagcggag caagtggctc cgcaaagcaa gaattaagac
     5881 cggccccggg cgacttgccg aagattatcc ctcggttttt cgcacgcctg gtatcacctg
     5941 ccggaaagct ggacctagcg tcgccgtcaa tgtcgatcga gccttcccga tcgcctgatc
     6001 gcccgccata accgccatcc ccagatggca ctcaagctga taccgacgaa gccgggcgcc
     6061 gaaatcgatc ccatattctc gcttaaccat ccgccgagca gcggcaaatc gctcagaagc
     6121 ggcggaactc gatgggccag catcaaagcg gagccgagaa acagggcact cgtcattagt
     6181 cccaccacaa ggcgattgac ggatggttct aaaccgcgat ggtcaagatg aatgtcaaac
     6241 gttccattcc gcaattgttg gacgacgtct cgtaaggcct gggggaggac ctcaagaagg
     6301 ctctgcaatt cccaataggt tcgctgcgct cgacgaaggg cgattcgtgg tgagaacaca
     6361 tgccaaagga ccttgcggcg atactggcgg atgacctcca agagatgaaa cctggggctt
     6421 agcagttgtg ccgtcccctc caacacgatg agagtcttta ggagaagggc aatcctcgcc
     6481 ggtaacacaa tccgataacg acggaggatt tcgatcatct ccgtcaaagc gccggcaagg
     6541 tcgaattgct cgattggcat gtgtccgtaa taggccacga agtcggccag atcgagggtc
     6601 aaagcagccc ggtccagcga aatcggcgtg gctccgacct ccgccacgat gatcgccaaa
     6661 cgttcactgt cttgactgac aagggcggtg agcaactcct gcactcgctc ccggagacga
     6721 tcgtccagtc gccccaccat cccaaaatcc aaaagaccga tcgtttcatc gggaaagacc
     6781 ataatgtttc caggatgggg atcggcgtga tagaggccat cttcgaaaat catcttgaga
     6841 taagcttgtg ccccacggcg ggcaacttcg tcaaagtcaa tgcccaattc tttgagccgg
     6901 tcctgctctg tcaacttgca accatcaagg tactccagga caagcagcct cttcgtgctc
     6961 aactccggga atgcccgcgg gatacggaca tccggcgaat cctcaaaatg agcccgaaat
     7021 tcctgcagat tgcgaagttc ccgaacgaaa tccacctccc ggaccagcgt ccttcgcaac
     7081 tctgcgacga ccgacatggg ccgatagatc tggaaatcgg gaattctctc agccagaaac
     7141 gctaaaccgc tgaggatttc cagatcggcc aatagttttc cttcgatgtc gggatgctgg
     7201 acctt
//
LOCUS       DAOSUT010000192         7011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5247_length_7011_cov_8.301179, whole genome shotgun sequence.
ACCESSION   DAOSUT010000192 DAOSUT010000000
VERSION     DAOSUT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5247_length_7011_cov_8.301179"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(857..1873)
                     /locus_tag="PLD05_13830"
     CDS             complement(857..1873)
                     /locus_tag="PLD05_13830"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78568.1"
                     /translation="MLIIKKRAYARAGLVGNPSDGYYGKTISVIVRNFWAEAVLYEWD
                     QLELVLSQEDRSRFDSIYDLAKDVRLHGYYGGIRLVKATIKRFVEYCDSQKISLPRRN
                     FSIRYQSNIPRQVGLAGSSAIIVAVLRCLMEFYGVDIPLRVQPSLALSVERDELGIAG
                     GLQDRVIQVYEGMVYMDFAREVMETIHGYPCGVYEPIDLAYLPPLYLAYSRAAAEPTE
                     VIHNDLRGRYERGEPAVREAMIQFAKLAEQGRKAILDKDWETLGRLMDSNFDLRRKIL
                     NLAPQHIEMVERARSLKVPAKFAGSGGAIVGLCPDESTFQKLREVMAEIDCEVLRPMV
                     APTS"
     assembly_gap    2009..2108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2109..2983)
                     /locus_tag="PLD05_13835"
     CDS             complement(<2109..2983)
                     /locus_tag="PLD05_13835"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOP78569.1"
                     /translation="MADQVVDIAVVPVAGRGTRLLPMTKSQPKEMLPVGRKPVVQYVV
                     EELVRCGIKRFLFITGPGKTAIENHFDLNPELIQMLRETGKEELLRELAFERAAVDYF
                     YTRQRRQLGLGHAVLCAEPMVDGQPFVVALGDSIIGLQAQSDIVARMIAVFETEKADA
                     VIAFETVPREDVIYYGIAEPEATQDTNGHFFALKGIIEKPSPETAPSCLAVAARYVFS
                     PVIFTHLKHTKPGKGEEIQLTDAIQRLIESGGKVLGMRLPEGERRFDIGNFESYFRAF
                     VEFALADEEYGEGLR"
     gene            complement(3466..3753)
                     /locus_tag="PLD05_13840"
     CDS             complement(3466..3753)
                     /locus_tag="PLD05_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78570.1"
                     /translation="MTENNQLPPSSAFNSTQDLAATWGETPVFVERRKNREGAPPAGG
                     ERRQFGNSYADLSPEAQELGRAIDAYKLQHHRRFINYEELLTVIKSLGYHK"
     gene            complement(4018..5658)
                     /gene="serA"
                     /locus_tag="PLD05_13845"
     CDS             complement(4018..5658)
                     /gene="serA"
                     /locus_tag="PLD05_13845"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOP78571.1"
                     /translation="MVKVVVLDPVSPEGLKLLEAAGTIEYEVRTGLKGEDLRKTLAEF
                     DGAICRSGVKITADALEGNKRLRVIVRAGVGTDNIDTAAATRQGIVVMNTPGGNTIAT
                     AEHTMALMLALSRNVFPAYKSLLEGRWERHQFVGTELCGKTLGLIGLGRVGQAVAERA
                     LAFKMRVLGYDPFLPEHRARELGVQMCHSIDEMLPEVDYLSVHTPLNDQTRNLIDRPQ
                     IQKMKKGVRLINCARGGIYNEEALLEGLQSGHIAGVALDVFTKEPCTASPLFGQPGVL
                     CTPHLGASTEEAQTNVAIEACQLLVDFFTTGAVRNSVNMAPLDPKTLEGLRGFLDLTY
                     RLGLLTAQLDREAPTRCRIRYQGEVANRDTRLLSAAFAAGLLQHALEQAVNLVNAPVL
                     MKQRGIEVTEERTTDIGDFASQITVEVFRNDKSVVAGGTLFGKSLPRLIQKGPYRLES
                     PLEGTMLIMYHRDVPGVIGRVGEICGRHNVNIAFLSLGRAENKPGGEAIGILALDNYP
                     PAEAIGEILKLDPIHRAIVVSLPPRGELPSWLGVGLPQ"
     gene            complement(5828..6682)
                     /locus_tag="PLD05_13850"
     CDS             complement(5828..6682)
                     /locus_tag="PLD05_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78572.1"
                     /translation="MKSLRQNVGREKLLASFAAAVLMVFLGTTQAFANGLPAGIAFFP
                     GMAPIDPVCGPLLTVAIAIIERPFFARAGLTPWALIHSLRANFASYLLGIVVTLIYVA
                     TVDAVIRAGFLNQDVFILLLPIVLISVTIWFEERLAVRVLAPTRKLRTRWIIAANVVS
                     NLALFAIMMCFGWIFSILGFHVYELIFWLEQWQAVFIVAYLLIFFGGISLALGVPLWK
                     LLPQGLQRKWGIPISVPETGFDNQVHETAPFEKASVASQAVSGEEIGNRVAEQPSPKA
                     QEPVRAGP"
BASE COUNT         1634 a         2066 c         1730 g         1481 t
ORIGIN      
        1 ccgcccacag cttgagttgt atcaccgagc ggcagccgag tggctccacg tgcctcaatc
       61 ccaagtctca gctcgaatcg cctttgttca ctcagggagt gtagttcggg tttgctgatc
      121 cccagagtgc aacgtcggtg ccgcggaggg ttactatttt acttcaggcc caatcggcgg
      181 aagcggaatg acgggctcta aaaggcgagg ctgttgttga gtctctgcct ttgtgacagt
      241 cctgaagctg atgaccgccg gggatgatgc cccttcgctg ttctgcgacg ggaattgcga
      301 tgtacttgac ctctcttctg gaacaggcga cggtagtcca cactcctgag gcacacccgc
      361 ggtgcatggc agacacgccc gacgaccgaa caggcacggc cctctcagcc cccagggaca
      421 ggagtgagtc ctagccattt tcgtctcgca ataaccatcc cagacgtgcc gacagcagga
      481 aggtggcagt tcacagcaac cttgtaagct gaccgttccg taggacggag cacaacatgc
      541 cggtgccccg agcgagttcc agtccgccgc aagaccggcg accactggga ttacgataat
      601 caatccggca agaaaccaac caaaccgagt ggccatagtg actcacctcc ctgttcggaa
      661 tctatccctc tcaagcgaag cctcggcaga tcgcagcagt gcatccacca tcacctcgac
      721 cctttgggga catcggctga caactaatag tagttatcct gttttcctag tccccctaaa
      781 atccgcgcga tccatcccac caataaatct cgcagaaggc gagtctcgcg cagagatgcc
      841 aaattttggc aggctgtcaa gatgttggcg cgaccatcgg acgtaagacc tcgcaatcga
      901 tctcggccat gacctcgcgg agcttttgga aggtggactc gtcgggacac aatccaacga
      961 tggcgccccc tgaaccggca aattttgcgg gaaccttaag gcttctcgcc cgttcgacca
     1021 tctcaatatg ctgcggcgca agattgagaa tcttgcggcg gagatcaaag ttactgtcca
     1081 tcaagcgtcc cagtgtttcc cagtctttat ccaaaatggc ttttcgcccc tgttctgcca
     1141 acttcgcaaa ctgtatcatc gcttccctga cggccggttc accccgctcg tagcgtcctc
     1201 gcaaatcgtt gtggatgacc tctgtcggct ccgcagccgc ccggctgtag gccaaataaa
     1261 gcggcggtaa ataggccagg tcaatgggtt catagacgcc gcacggatag ccgtgaatcg
     1321 tttccatcac ctcgcgtgca aaatccatat aaaccatgcc ctcatacact tggatcaccc
     1381 ggtcttgtaa accgccggcg atgccaagct catccctttc cacagagagg gccaaggagg
     1441 gttgtacgcg aagcggaatg tcaacaccgt agaactccat caaacatcgc aacaccgcaa
     1501 cgatgatggc gctggagccc gccaggccaa cctgtcgtgg aatattgctt tgataccgaa
     1561 tagaaaagtt ccggcgaggc agcgaaatct tttgcgaatc gcagtattca acaaaccgtt
     1621 tgatcgtcgc cttgactagg cgtattcccc cgtagtaacc gtgcaggcgg acgtccttgg
     1681 ccaagtcgta aatcgaatca aaccggcttc tgtcctcttg gctcaagacc aactcaagtt
     1741 ggtcccattc atagaggacg gcttccgccc aaaaattgcg gacgatcacc gaaatcgtct
     1801 tgccgtagta cccatcggac gggtttccca ccaaacccgc cctggcgtag gcgcgttttt
     1861 ttatgatgag cattgagata aaccctcttg cccgaaaatc ggctcaagac catcctgagg
     1921 ggatgaattc acctcatgag atgcttaatg tactcgcgca acccctcgcc gtattcttcg
     1981 tccgccaacg caaattccac aaaggcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnntc gcgcaacccc tcgccgtatt cttcgtccgc caacgcaaat tccacaaagg
     2161 cccgaaaata gctctcgaag ttcccaatgt cgaaacgtcg ctcgccctct ggtagtcgca
     2221 tccccaagac ctttcctccc gattcaatta accgttgaat cgcatcggtc agctgaatct
     2281 cctctccttt gccgggttta gtgtgtttca aatgggtaaa aatcacgggg gaaaagacat
     2341 atcgggcggc gacggccagg caactcggcg ccgtttcggg agatggcttt tcaatgatcc
     2401 cttttaaggc aaagaaatgc ccgttcgtat cctgcgtcgc ctctggctct gcaatgccat
     2461 agtagatgac gtcttcgcgt ggcaccgtct caaatgcgat gactgcatcg gccttctctg
     2521 tttcaaaaac cgcaatcatc cttgccacaa tgtcagattg ggcttgaaga ccgatgatgg
     2581 agtcgccgag ggcgacgaca aatggttggc cgtccaccat cggttccgca cacaaaacgg
     2641 catgtcccag tcccaattgc cgcctctggc gggtataaaa ataatccacg gccgcccgtt
     2701 caaaggccaa ttcgcgaagc aactcctctt tgccggtctc ccgcagcatc tgaatcaact
     2761 ccgggttaag atcgaagtga ttctcgattg ccgtcttccc ggggcccgta ataaagagaa
     2821 atcgcttgat cccacaacgg actaattcct ccaccacgta ttgaacaacc ggctttcgac
     2881 caaccggaag catctccttg ggttggcttt tcgtcatcgg gagaagccgt gtccctcgac
     2941 ccgccacggg gaccaccgcg atatcaacaa cctgatctgc catagaaaaa ccttttcctg
     3001 ttattgaact cccacgtgca atcgcgaagg gctgcgcagg gtggttcgga gccccctctg
     3061 ccaagctacc ggcccgacat tgtgaacgat cgcgggctgg tcttcagaaa aacccctcgc
     3121 cgacgccggt caccgatcct actacttaac tataacttct cacgaggcag aatgatcggt
     3181 gacggcaaat cgaagacgag tgatcactca cagaatggct tcgcggagtg ccggcaaact
     3241 gaccccgctt tgctaagagc gtatcccata atctcctttc accgcttccg cgagagacgg
     3301 gtcgggcgta agggtggctc gaatcgttac tggttttctc aattgattgc gacgttcact
     3361 tgccctcacc ccaaccctct cccggaggga gagggggaac ttttcgggat aggctctaat
     3421 agacaagaga tgggttacgc acagcaattc cccggggaac acgggttact tgtgataccc
     3481 aagtgatttg atgacggtca gcaattcttc gtaattgatg aatcgccggt ggtgctgcaa
     3541 tttataggca tcgattgcac gaccgagttc ctgggcctcg ggcgacaaat cggcgtaact
     3601 atttccaaac tgccgccgtt ctccccctgc tggcggggcc ccctctcgat tcttgcgcct
     3661 ttcgacaaaa acaggcgttt ccccccaagt tgcggccaaa tcctgggttg aattgaacgc
     3721 gcttgaggga ggtaactggt tgttctcggt catggcccag ggtcctcgtc acgcaaggtt
     3781 tcttttaacc tcggcagcag tccgtgtcat cgctagcacg atgcgggtaa agatcgctcc
     3841 atgtaagggt aatcggcgag catgtgtccg ctcatccagc gttctctgcc caaagataca
     3901 cgtgctatag caagaatagc ttacacggaa ccaccatagg aaacgtccag aaaaaacgac
     3961 gaggaagcgg actcggctcc ctcgtcgcca acagtactac gcgtttatac cgatacgtca
     4021 ctgcggtagg ccaaccccca accatgaggg aagctctccc ctcggcggta ggctgaccac
     4081 aatagcccta tggattgggt caagtttgag gatttctccg atggcctcag caggtgggta
     4141 gttatcgagg gcgaggattc cgatagcctc gccgcccggc ttgttttcgg cacgtcccag
     4201 cgaaagaaag gcgatattga cgttatggcg tccgcaaatc tcgccaactc gcccaatgac
     4261 cccgggaacg tcccgatggt acataatgag cattgtcccc tcgagggggc tttctagtcg
     4321 atagggccct ttttggatca accgtggcaa tgacttccca aacagcgtcc caccggcaac
     4381 gactgacttg tcattacgga agacctccac cgtaatctgc gatgcgaaat caccgatgtc
     4441 agtcgtccgt tcctcagtca cctcaatacc ccgctgcttc atcagcacgg gggcattaac
     4501 cagattaaca gcctgttcca aggcatgctg gaggagtccc gccgcaaacg ccgccgataa
     4561 caatcgcgta tcccgatttg caacttcccc ttgataacga atacggcaac gcgtcggcgc
     4621 ttcccgatct agttgagccg tgagtaagcc cagtcggtaa gtaaggtcca aaaagccgcg
     4681 caacccttcc agagtcttcg gatcgagggg ggccatattg accgaattgc gaaccgcccc
     4741 cgtggtgaag aagtccacca aaagctggca ggcctcgatg gcaacgttcg tctgggcctc
     4801 ttcggtgctg gctcccaggt gcggcgtgca gagcaccccg ggctgcccaa acaatggact
     4861 tgctgtgcac ggctctttgg tgaacacgtc gagcgccaca ccggcgatat gtcccgactg
     4921 taaaccttcc aggagtgcct cttcgttgta aatgccaccc cgtgcgcagt taatgagacg
     4981 gacgcctttt ttcatcttct ggatttgcgg gcgatcaatc agatttcgcg tctgatcatt
     5041 tagcggtgta tgaacgctga ggtagtcaac ctctggcagc atttcatcga tgctgtggca
     5101 catctgtaca cccaattcgc gggcacgatg ttctggaagg aacgggtcgt agccgaggac
     5161 ccgcatcttg aaggccaagg cccgctcggc aacggcctgc ccgacacgtc ctagtccgat
     5221 cagaccaagc gtttttccgc acaactccgt acccacaaac tgatgccgct cccatctccc
     5281 ttccaataga ctcttgtaag cgggaaagac attacgggac aaggccagca tgagggccat
     5341 cgtatgctcg gccgtggcga tcgtgttccc gccgggcgtg ttcatgacaa cgataccctg
     5401 tcgcgtagcc gccgcggtat cgatgttatc cgtcccgaca cccgcccgca caatcaccct
     5461 cagacgcttg ttcccctcca aagcatccgc cgtaatcttg acaccacttc ggcagatcgc
     5521 gccgtcgaat tccgccaacg tcttgcgaag gtcttcgccc ttaagacccg ttcttacctc
     5581 gtattcgatc gtaccggccg cttccaatag cttgagaccc tcgggcgaaa ccggatccaa
     5641 aacaaccacc tttaccatgg tcagaccctc aatttcaaac acaatgcaaa aaggagccca
     5701 taaccgtgaa ggcaccaagc ggcgcacgga aactcgttac ggcgtcgcag taccgcgcac
     5761 gcggaccaac ggtgtcctcc tcatcccggg cataacccat tccttttacg acgagtctct
     5821 cggggcgtca aggacccgcg cgaacaggct cctgcgcttt aggggatggc tgctccgcaa
     5881 cgcgatttcc aatctcttcg ccggacaccg cctgagaagc cacagaggcc ttttcaaaag
     5941 gtgcggtttc gtgcacctga ttgtcaaacc ccgtctccgg cacagagatg gggatacccc
     6001 attttcgctg aaggccctga ggtaaaagct tccaaagggg aacgcccaag gccaaggaaa
     6061 tgcccccgaa gaaaatcaaa agatacgcga cgataaatac agcctgccat tgttctagcc
     6121 aaaagataag ttcataaacg tgaaacccta agattgaaaa gatccagcca aaacacatca
     6181 tgatcgcgaa taaagcaagg ttgcttacca cattggccgc aatgatccag cgcgtgcgga
     6241 gtttgcgtgt cggtgccaag acgcgaaccg cgagtctttc ttcgaaccaa atagtgacgc
     6301 taatcaacac tatgggaagc aaaagaatga agacatcctg attgaggaag cccgcacgta
     6361 tgaccgcgtc gaccgtcgca acataaatca gggtgaccac aatgccaagc aaatagctgg
     6421 caaaattcgc ccgcagggag tgaataagtg cccaaggagt caaccctgct cgagcgaaaa
     6481 atggccgctc gatgatcgca atcgcgacgg tcagcaaagg cccacacacc ggatcaatcg
     6541 gcgccatccc cggaaagaaa gcaatccccg ccggtaggcc gttggcaaag gcctgcgttg
     6601 ttccaagaaa aaccatcaaa actgcggccg caaaactagc caagagtttc tcgcggccca
     6661 cattctgccg aagagacttc attccccacc tccgcgctag tcggcatgaa gtgcaatttt
     6721 gccacgatcg aagtcaccgc ccgctatttt atcacatacc ttcgcgacgt ttacagtcga
     6781 ataggttttt ttgcgagagt gacttcacct cggacaatcc tagggtgtgg cattctggaa
     6841 cggacggtga tacgttattt gttgatcacg cagttcaagt gcaagttcca ttggcaagca
     6901 gcaaggagaa acaggttcat gagaagcaca cctattgttg ccatcacttg ggttgtggca
     6961 tggttggccc cgattcttat cgtctttgta tcatacggcc aagagcctca g
//
LOCUS       DAOSUT010000193         6920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5323_length_6920_cov_8.265404, whole genome shotgun sequence.
ACCESSION   DAOSUT010000193 DAOSUT010000000
VERSION     DAOSUT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5323_length_6920_cov_8.265404"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLD05_13855"
     CDS             complement(<1..896)
                     /locus_tag="PLD05_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP78573.1"
                     /translation="MSDVAIRVDNLSKKFYLGERVGIKTFREALQDLVTAPFRVWKKN
                     GNGQDQEKVDTDREIWALKGVSFEVKHGEVLGIIGPNGAGKSTLLKILSRITEPTGGY
                     AEIRGRVGSLLEVGTGFHPELTGRENVFLNGAILGMSKAEIRRKFDDIVAFAGVEKFI
                     DTPVKRYSSGMQVRLAFAVAAHLDPEILIIDEVLAVGDAEFQKRCLGKMNEVSRSGRT
                     VLFVSHNMAAVEALCTKAMVLSSGELLAHTLVDKAILSYLESVRHAQQMACQSRYMAA
                     FAIKDAEGRDRTTFRVGETVEF"
     gene            complement(1147..2205)
                     /locus_tag="PLD05_13860"
     CDS             complement(1147..2205)
                     /locus_tag="PLD05_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOP78574.1"
                     /translation="MRTDPIIPDSRPTEAELTADRPPEKAGLGEDGVNVTADLTAEVA
                     DDWQDRDVVADSEAQPAQADSVPPLPKDLPVTIIRPTGGWRALDLREVWRYRELMLTL
                     AWREITIRYKQTFLGAAWAIIQPLMTTGIFSVLFALLLGKGNEPTAGNVPYAVSTFCA
                     MLPWQLFATSLNQSGNILIQYRSMITKIYFPRLILPMAAVLSSLVDFGIAFVALLALM
                     AFYGIFPGWGLLTLPFFVALAVIASLSVGLWLAALNAIYRDFRYVIPFIIQIGMFVSP
                     VVYSMESIQGRLSPWAWTLYSLNPMAGVIEGFRWALLGTSHGPGPLLALSALATTIIL
                     VCGMFYFRRMERLFADVI"
     gene            complement(2240..2887)
                     /locus_tag="PLD05_13865"
     CDS             complement(2240..2887)
                     /locus_tag="PLD05_13865"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOP78575.1"
                     /translation="MLLRAGLVALGLLVIGIGARWAASQAVPNIVRVPEQEQLAAFPK
                     QLGPWKCEDEEKIDAKMFQALGTGVAVTRRYSQSAERFLEMHAAVFTQPGFSLPHPPE
                     LCYSGTGWRVRSTRDVPLTLIDGTSGSVRFIRLIKERGDQQATVLYCYQLAGPFSADR
                     DKVRTFFWKFRGQKSRPPLVKLMLHLPGSGETIENLGLDFAQRALEKLAEMEKTW"
     gene            complement(3031..4719)
                     /locus_tag="PLD05_13870"
     CDS             complement(3031..4719)
                     /locus_tag="PLD05_13870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOP78576.1"
                     /translation="MTPPMAVYAAVSCLAAMIASAVFTRFMCWVAPKIGFVDQPDRNR
                     KVHKKATPLGGGLAIYLAICLVLGVNICLANPWGLRVHQDWWDLVVLFIAGSWIVGLG
                     LIDDWSRLRGRYKLAGQFVAAAILVAGGYTFSSFRLFGISVELGWIGAVVALFWFLAT
                     INSINLLDGMDGQAATLGTIFSITVGIMACVTGHYAVAFVAFVLAGATFGFLWFNLPP
                     ARIFLGDAGSMLIGLMLGVLAARASLKGPSTFLLAAAVALWTLPFLDCFAAIIRRTLS
                     GRSIYAADRSHIHHCLMEKCKSSETALMVISACAAVTAVASLGGLVLGNDTIALIAAS
                     GVVAFLIVTGLFGRSECRLILNTLRRWGDGLRRWLLGQEGKCLCNGTHFQGKFDWHKL
                     WTHLTDPAGEYNVIYLRVDLNDPRIQEGYCGEWKGPVFPNPDDTPISHHEFPLCTGDR
                     VVGKIKAVLRRSAETFLRDLEFVISLVDPFEQQLEQLNDERASQERADKEHSVGTHGS
                     HIFKNVLPTNSIKMTIPGNGDGNGKRSLISHGAETTKTPSIRDLSGGKPTESRK"
     gene            complement(4980..>6920)
                     /locus_tag="PLD05_13875"
     CDS             complement(4980..>6920)
                     /locus_tag="PLD05_13875"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOP78577.1"
                     /translation="TQLEYLRSRFVLIAALRQPEVSRLPSIQEQLDPVQWVAQNLSVR
                     FPGNSEFMNVSFSSTRPEETAVLVNAIVDAYMREVVDTEQRERRERLSELDQLYAEKE
                     SELRKLENDLKTLSQQLETGDSQALSLKQQITLQQFSTYRSELINVQLRIMRAQGELS
                     LKKAIREKAAQIEITERELDAAAKTDPITLQLLMREAELKNLVAQTRDVVRPEMAGRF
                     GNRYVQDLADVQARIKARREELRQELEQKRVASIDEEIESLNFELAILNQQQQQLQQE
                     VENVRRVAETFGKGSIEVEFLRAEVELARKTVQTIAEERNRMSIELRAKPRITVVQRA
                     EAPRVADQDRRVQLSVLAGLAGLVLPGITLLWWDTRRKPVNTPQEIRHVAGAEVWGTI
                     PRLPVRVMRAIGNGRVRSKKHRRWQRMFVEAVRIVAARLVRESQDERLNVIMVTSAVA
                     GEGKTTLAAQLALSLAQIGKKTLVADFDLRRPAIHDVFDVPISPGVSEVLRGEVSLQD
                     AVRATSVENLYVLPAGYWSPHRLAVLANGVVGATFQQLREDFDFIIVDGTPVLPVADT
                     RFISTHTDGVVLSVIRDYSRTPLVRSAKQILESFRVRVLGTVVTGSTEEVYHSYSYHD
                     LVDGTSSALSDGEEVESEKADT"
BASE COUNT         1584 a         1975 c         1866 g         1495 t
ORIGIN      
        1 tgaaattcga ctgtctcacc aacccgaaac gtagtgcgat cccgtccctc cgcgtcctta
       61 attgcaaagg ctgccatata acgcgactga catgccattt gttgagcgtg acggacgctt
      121 tcaagataac taagtattgc cttgtcgacc aaggtatggg caagcagttc ccccgaagag
      181 agcaccatgg ccttcgtaca caacgcctcc actgcggcca tgttgtggct gacgaagagg
      241 acggtgcggc cggagcggga gacctcgttc atcttgccca ggcatcgctt ctgaaactcg
      301 gcgtcgccga cggccaggac ctcgtcaatg ataaggattt cggggtccag gtgggcggcc
      361 acagcaaatg ccagcctgac ctgcatcccg ctagaataac gtttgaccgg ggtgtcaata
      421 aacttctcga ctccggcaaa ggcgacgatg tcgtcgaact tgcggcgaat ctcggccttc
      481 gacatgccca agatggcccc gttgaggaac acgttctcgc gtccggtcag ctcggggtgg
      541 aagcccgtgc caacctccag caggcttccc acccgaccac ggatttccgc ataaccgccc
      601 gtcggctccg tgatgcggct gaggattttc aagagcgtac ttttgcccgc accattggga
      661 ccgatgatgc ccagcacctc gccatgttta acctcaaagg aaacgccttt cagggcccag
      721 atctcacggt cggtatcgac cttctcttga tcttggccgt tgccgttctt cttccaaacg
      781 cggaagggag ccgttacgag gtcctggagg gcctcgcgaa acgtcttaat accgacgcgc
      841 tcgcccaggt aaaacttctt gctaagatta tcaacgcgaa tggcaacgtc agacatggcc
      901 ggtgtgtgct aagcggacga tcgcataagt gacagcgcga gctttgaaca aagacgagtg
      961 agcccagagc gagaactgcc gaaccagaga attggcaaaa acctcaaaat cggtatccca
     1021 gcgcagcctc cggccccttg ggttgcgccg ttactcttgc atcccacaga ggtcatcatg
     1081 ttttaattta gcttacgata tggacggggc gcagcgaaaa cggggaatgc ttacgcccaa
     1141 gacgtatcaa ataacatctg caaaaagccg ttccatacgg cggaagtaga acattccaca
     1201 cacaaggatg atcgtcgttg ccaacgccga aagggccaag agcggacccg ggccgtgaga
     1261 tgttcccagg agtgcccagc gaaagccttc aatcacgccg gccataggat tgagggaata
     1321 caaagtccac gcccacgggg agagtcgtcc ttggatggat tccatgctat agaccactgg
     1381 gctaacgaac atgccaattt gaatgataaa cgggatcacg taacgaaaat cccgataaat
     1441 ggcgttaagt gccgccagcc acagtcccac ggagagtgag gcaatcaccg ccaatgctac
     1501 gaagaacgga agggtcaaaa gcccccaacc aggaaaaatc ccataaaagg ccatgagggc
     1561 aagcaaggca acaaaggcaa tgccaaagtc caccaaagag gacagcacgg ccgccatggg
     1621 gagaatcaat cgcgggaagt aaatcttcgt gatcatgctg cggtactgaa tcaggatgtt
     1681 tcccgactga ttgagggagg tggcaaaaag ctgccaaggc aacatggcac aaaaggtgga
     1741 aacagcgtag gggacattgc cggccgtcgg ctcatttccc ttgcctaaaa ggagggcgaa
     1801 gaggacgcta aaaatgcctg tcgtcataag cggttgaatg atggcccagg cagccccgag
     1861 aaacgtttgt ttgtaacgaa tggtgatttc acgccaggcc aaggtgagca tcaattcgcg
     1921 gtaccgccag acctcacgga ggtccaaggc tcgccatccg cccgtcggcc gaatgatcgt
     1981 gaccggcagg tccttcggga gcggagggac actatccgcc tgggcgggct gggcctcgga
     2041 gtcggcgacg acgtccctgt cttgccaatc gtcggcaacc tctgcggtta agtcggctgt
     2101 gacgttaacg ccgtcttcac cgagcccggc tttttccggc ggtcggtccg cagttaattc
     2161 tgcctctgtc ggcctggagt ctgggataat gggatcggtt cgcattttta ttgtaagcga
     2221 aagaagtgcg ctgtttattt caccaagttt tttccatctc ggccagtttc tcgagcgcgc
     2281 gttgggcgaa atcaaggccg aggttctcta tggtttcccc acttccgggg agatgcagca
     2341 ttagctttac caatggtgga cgcgatttct gtccgcggaa cttccagaaa aatgtccgca
     2401 ctttgtcgcg atcagcagaa aacggtcctg ccaattgata acaatatagc acagttgcct
     2461 gctggtcacc gcgttctttt atcaaacgga taaaacgtac gctcccggat gtgccgtcga
     2521 tgagggttag tgggacatcg cgggtcgatc ggaccctcca gcctgtcccc gagtagcaca
     2581 gctctggcgg gtggggcaag ctgaaaccag gttgcgtgaa gaccgcggca tgcatttcga
     2641 gaaatcgttc cgccgactgg ctgtatcgac gcgtcacggc aacaccggtt cccaatgctt
     2701 gaaacatttt agcatcgatc ttctcttcgt cttcacattt ccaagggccc aattgcttgg
     2761 gaaaggcggc cagttgttct tgctcgggca ctcgcacgat atttggcaca gcctgagaag
     2821 ctgcccaacg tgcgccaatc ccgatcacaa ggagccccag cgctaccaga ccggcccgaa
     2881 ggagcaagct cagcccccgc cggcgttttt gcgcggcatt ggtggctgaa tccctcaggt
     2941 gggatgtctt ggtcgagtgg cccgaaagct ccccggtttt cgtagtccct ggcgggccgt
     3001 gacgagtagc cgatcctttg cctgccgccc tcatttccta ctctccgttg gtttgcctcc
     3061 gcttaagtcc cgtatcgatg gcgttttagt cgtttctgcg ccgtgggaaa tgagtgatct
     3121 ttttccattt ccgtcgccgt tgccgggaat ggtcatcttt atggagtttg tgggaaggac
     3181 gttcttgaaa atgtgcgatc catgtgttcc gaccgaatgc tccttatcgg cgcgctcttg
     3241 gcttgccctc tcgtcgttga gttgctcaag ctgctgctca aacggatcaa cgagactgat
     3301 gacgaactcc aaatccctta ggaatgtttc tgctgagcgc cgaaggactg ccttgatctt
     3361 tccaacgacg cgatccccag tacacagagg gaattcatgg tgactgatcg gcgtatcgtc
     3421 gggattggga aatacaggac ctttccattc gccgcaatag ccctcttgga ttcgtgggtc
     3481 attgagatca acgcggagat aaatcacgtt atattcacca gccgggtctg tgagatgtgt
     3541 ccaaagcttg tgccaatcga actttccttg aaaatgggtg ccgttgcaca ggcacttacc
     3601 ctcttgtccg agtagccatc tgcgaagacc gtcaccccag cggcgaagtg tattgagtat
     3661 gagacggcac tccgatcggc caaagagccc tgtgacgatc aaaaaggcta ccacgccgct
     3721 ggccgcaatg agggcgattg tgtcgtttcc cagaaccagt ccccccaagg atgcaacggc
     3781 tgtaaccgca gcgcatgcgg aaatgaccat cagggcggtt tcactgcttt tgcacttctc
     3841 catcaggcaa tggtgaatgt gactacgatc cgcggcatag atgctgcgac ccgataaagt
     3901 tcgccgaatg atagccgcaa agcagtcgag gaacggaagc gtccagaggg cgactgccgc
     3961 tgccaaaagg aaagtactcg gacctttaag cgaagcccga gcagcaagca caccgagcat
     4021 caagccgatt aacatacttc cagcgtcacc caaaaagatt cttgccgggg ggaggttaaa
     4081 ccaaaggaag ccgaacgttg ccccggcaag aacgaacgcc acaaaagcaa ccgcgtaatg
     4141 tccggtgaca caagccatga ttcccacagt gatgctgaat atggtcccca gcgtggcggc
     4201 ctgaccatcc attccatcca ggaggttaat cgagttgatg gtcgcgagga accagaaaag
     4261 ggccacaacg gctcctatcc aacctaattc gactgatatg ccaaaaagcc gaaaactgct
     4321 gaacgtatac ccgccagcca ctaaaatcgc tgcggccacg aactggcccg ccagtttgta
     4381 tcgtccgcga agccgagacc aatcgtcaat cagcccgagc ccgacaatcc aacttcccgc
     4441 gatgaagagc accacgagat cccaccaatc ttggtggact cgcaaacccc acggattggc
     4501 caaacagata ttaaccccaa ggacaagaca aatcgctaag tagattgcga ggccaccgcc
     4561 caacggcgta gctttcttgt gaactttgcg attgcgatct ggctgatcga caaacccgat
     4621 cttgggggcc acccagcaca tgaatcgcgt gaaaacggcg ctggcaatca tggccgctag
     4681 gcagctcacg gcggcataaa cggccatcgg tggcgtcatt gatgcgtctc cctcggaggt
     4741 tgaatcacca tggttgcgaa gtctaacacg tccccgcccc aaatcatctt gcatttacac
     4801 aacgtcttca tggacgtgtg gccgttcctt gcctaaaaac gggaaatcaa cagcgaaaac
     4861 cgctcttgat ttacgactcg atctcgccaa cgtttgacaa ttggtcatgt taggtgttac
     4921 ccgcgtgact ccttttcttc accggcgcga tttcgcaccg acattttgtc cgcgaggtat
     4981 taagtgtcgg ctttctcaga ttcaacttcc tccccgtcgc ttagggcact actcgtgccg
     5041 tccacgagat catggtaaga gtaggagtga tagacctctt ctgtcgaacc cgtgaccacg
     5101 gtacctagga cacggacccg gaaactttcc agaatctgct ttgcggaacg cacgagcggt
     5161 gtgcgactgt agtcccgaat gaccgacaac acaacgccat ctgtgtgggt actgatgaat
     5221 cgcgtatcgg ctaccgggag gacaggcgtt ccatccacga tgatgaaatc gaaatcctcc
     5281 cgcaattgct ggaatgtcgc gcccacgacg ccgttggcaa gcacagctag gcgatggggc
     5341 gaccagtaac ccgccggcaa gacatacagg ttttcgaccg aggtcgctcg gacggcatct
     5401 tgaagcgaaa cttctccccg taagacttcg ctgacaccgg ggctgatagg gacatcaaac
     5461 acgtcatgaa tggcaggccg acgcaggtcg aaatcggcga ccaaggtctt cttgccgatc
     5521 tgggccaaac ttagggcgag ctgtgccgca agtgtggtct taccctcacc ggctacagca
     5581 ctggtgacca tgatgacgtt tagtcgctcg tcttgggatt cgcggaccaa tcgagccgcc
     5641 acaattcgca cagcttcgac aaacattcgc tgccaccggc ggtgtttctt tgaacgcact
     5701 cgcccattgc cgatggcccg catcacccga actggcaaac gaggaatcgt cccccagacc
     5761 tccgcccctg caacgtggcg gatctcttgc ggcgtattga caggctttcg acgggtgtcc
     5821 caccacagaa gtgtgatccc gggaaggacc aatccggcca aaccggcaag gacagaaagt
     5881 tgaacacgcc tgtcttggtc tgccacccga ggtgcttccg ccctttgaac aaccgtaatc
     5941 cgcggcttgg cccgaagctc gatactcatg cggtttcgct cttctgcgat ggtttggacg
     6001 gtcttgcggg ccagttcgac ctcagcccgc aagaactcga cctcaatcga gcccttgcca
     6061 aaggtctcgg caacccggcg gacattttcg acctcttgct gcagctgctg ctgttgctgg
     6121 ttaagaatgg ccagttcgaa gttgaggctt tcgatctctt catcgatact cgctacccgt
     6181 ttttgttcca attcttgccg gagttcttca cgtcgggcct tgattcgtgc ttggacatcg
     6241 gcaaggtcct ggacgtaacg gttgccaaag cgcccggcca tttccggacg gaccacatcg
     6301 cgggtttgtg cgaccaggtt cttgagctcg gcttcccgca ttaggagttg cagagttata
     6361 ggatcggttt ttgcagcagc gtcaagttcg cgctccgtga tctcaatctg agctgccttt
     6421 tcgcggattg ccttctttag ggaaagctct ccctgagccc gcatgatccg caattgcaca
     6481 ttgattagct cgctacgata ggttgagaac tgctgaagcg taatctgctg cttgaggctg
     6541 agagcctggc tgtcccccgt ttctaattgc tggctaaggg tcttgaggtc gttttccaac
     6601 ttccgcagtt cgctttcctt ttcggcgtac agttggtcca attcgctcag tcgctctcga
     6661 cgctcgcgct gttctgtgtc aaccacctcg cgcatgtacg catcgacgat ggcgttaacc
     6721 aacacggccg tctcctccgg tcgcgtgctg gaaaaactca cgttcatgaa ttccgaattc
     6781 ccaggaaaac ggaccgaaag attttgggct acccattgca cggggtcaag ttgctcctgg
     6841 atggagggta gccgagacac ctctggttgg cgaagggccg cgattaagac aaaacggctg
     6901 cggagatact caagttgcgt
//
LOCUS       DAOSUT010000194         6835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5386_length_6835_cov_10.269469, whole genome shotgun sequence.
ACCESSION   DAOSUT010000194 DAOSUT010000000
VERSION     DAOSUT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_6835_cov_10.269469"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLD05_13880"
     CDS             <1..218
                     /locus_tag="PLD05_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78578.1"
                     /translation="GSTANRNDRKLMWHPRKVIRGTARLTSTIVVVLNMVWVGHIFCE
                     VVIRFYCPVLKWQGLYWIGALAVIDQN"
     gene            complement(757..3144)
                     /locus_tag="PLD05_13885"
     CDS             complement(757..3144)
                     /locus_tag="PLD05_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP78579.1"
                     /translation="MSGPQTSQPFDPYYTWLGIPPSERPISYYRLLGLKPFESNETAI
                     EAAVDQRLAYLRTKALGPHAAFAEKLISEITQARLVLLDPVRKQAYDAALSSTAHEGP
                     GLSQQEAPARHSGTPRVAAPLGTGSYMPGNVRRFRRKSRLTKDQWIAALGLGLGLCFV
                     IGVLVLWLSGKKIGSQGDRNPSPRQEQLAGGPPAPRVNDGSPADAGARLHGLPQPGRI
                     PGANPPESLPRQAPPEPPRAPDAEAKPAVNPMANPAENNRWLEKVRRATVVIQGDRGQ
                     GSGFFVTTGAGKPVVVTNAHVIRDNFNLRLKLFDGREIPVQQGAVLADRDLAILAVEA
                     INLPQPLELRGELPQVAEQVFAYGAPQGLTGTLTAGIVSAVRSSSEIRDVVDDGSFDK
                     PVRKAEVRWIQTTAPISPGNSGGPLLDQQGRVVGINSLAFNNLLGQNLNFALSSTEVL
                     PHLAAPQFAALPKSAPIGPGVFGVNQPQGGTPKSSPNSLYPFEQYSCQDITLPSGAVF
                     QPGKVRPPEKWRLAIESGHVLVCRRGQITVSAKKEVGGGLFREDVSFGVGAVGEEGAA
                     YIGGSRTRYHYTPGVVSPFAFYVFYDDNDVMRAAMAFEKGLLNGPAVLFDENGHPYVI
                     ANYYRGTRTGPLVVYDEEQWPAVYVEFTRNRKHGLVCVFDQHLPSWIENWRANQLVEA
                     CLIKWEGNQCQVLLASALTNPDDQALSQERMAKVSRYLDELDSLESELKRNVAEAFRW
                     ADNEIKKRRAAQFSAAATAAILARHRAREAAGAALRAEANARFQTGSGPPPLPIP"
     gene            3478..4326
                     /locus_tag="PLD05_13890"
     CDS             3478..4326
                     /locus_tag="PLD05_13890"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOP78580.1"
                     /translation="MQTPDDGIEITRVLAETFLVDGEYHQTIDSTNNRAKALAGSGQI
                     GLPYLVLAEEQTAGRGRALRRWWTGRGSLAFSIILPLTEERPVNRLSEKENSTSRARP
                     KAFLCEDGIRLLGLAGALAVYRAASRHLPSDISLGIHWPNDVYANDRKLAGVLVEITG
                     NQRAVVGIGVNVNNRSEDAPPHVQHKVATLRELCGQPQNRTELLIDILQEFEPLFELL
                     KNQPERLAEEANRVCLQKGRELVLEVEQGLVTGLCLGIDLTGALVLKTVSGERRFLSG
                     TVVAVE"
     gene            4469..5860
                     /locus_tag="PLD05_13895"
     CDS             4469..5860
                     /locus_tag="PLD05_13895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOP78581.1"
                     /translation="MNQGLVAREMLDWEFLETYLRQRDAHFAVHWEWITEVRHKLEAN
                     AGLDRGAVTDDVARMSERIERLRYQLLQERSDPDAADYQAAEVLDSLFAILTDRETTT
                     PRHLQQVLEDDTFAVEAIKSLEWCLNPDVPLDTVIASARRLTKEHFGANLPDQGAESA
                     SWPIFLYAPLYLSSYCVNHCLYCGFRFPETTPRRLLSADEAVEQVGLLVGRGMRHILL
                     VAGDYPQKTDIDYYVETITAIRRRWPVSLAVEIAPQSTGGYRSLVKAGVIGVTLYQET
                     YDRSLYRTYHIRGPKAHYAWRLEGLERAAEAGMRRLGLGILLGLGDPVSDARAMVRHA
                     YYLKERFPDRRIAFSLPRIHEGPEGFQIPNPVNDETLIRLYCGLRHAMPGAELVLSTR
                     EMPQLRDLLAATCITQLSAGSSTVPGGYDEMGTHFGDEHDGQFRVVDRRSVNDVIAVL
                     KSQGHAIIWQPRE"
     gene            6189..>6835
                     /locus_tag="PLD05_13900"
     CDS             6189..>6835
                     /locus_tag="PLD05_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP78582.1"
                     /translation="MFVAATTRCFANLPLEAAFERLNDLEFGYVEIMLHESDGHLKPS
                     EVAADLDRAIALCRKTYRLTPIAYSVDIEAQGDEYYRQFAACCKLAKATRVVTVVVRA
                     SELGTPFNEEVERLQNLVAIATMEEVVVALLTERGRMSELPETAAVLCHHAKGLGICL
                     DPSHLIYGRETPLNPEPVLKYVRHVRLRDTRKDKFQVLVGQGEVEYGKLLANLGR"
BASE COUNT         1513 a         1896 c         1931 g         1495 t
ORIGIN      
        1 gaggttcaac ggcgaatcgt aacgatcgca aactaatgtg gcacccccgg aaagtaatcc
       61 gtggaacggc acgacttacc tcaaccattg ttgtcgtgtt aaacatggtc tgggttggac
      121 acattttttg cgaagtggtc atccgttttt attgtcccgt gttgaagtgg cagggcctgt
      181 actggattgg agcgctagca gtgattgacc aaaattaggg gagcaacacg ggtcgcccca
      241 agttaggaga accacggggt tgcgaggagc tgggcaacca cagggggttg cccctacaca
      301 cgcaattaaa agccgtaggg gcaggccccc gtgcctgccc attctctttc tcttgcctgc
      361 gcgaaaccaa cgggctccga cgctcttcgg caagttaagc tcggcgaaat cttgtcaagc
      421 cttttctggc cctgtttatt tcccgaccct gcccgaagca tccggccgct cgggtagact
      481 ttctttcgcg cgctttcggg ggaaagcaac tttggggaat cacgtcagat atgaccatag
      541 aacgccgagg acggcgtccc tcccaacgcc aaacgcgcgg agggcgcccc ttccaacttg
      601 tcaaccgata cgacgaacag tcggtagcac acggcgggtc cggcagagtg agtgcctaca
      661 aaatgccggt cgccggggat ggcgtccctt cccaaaaccg cggcgctagc gggttacgct
      721 ccgtcgtggc cggatcaatc gcagccccat tggttactag ggtatcggaa gcggcggcgg
      781 acccgaaccc gtctggaaac gggcgttggc ctcggcacga agcgcagcac cggcggcttc
      841 tcgggcgcga tgtcgggcaa ggatcgctgc tgtggctgcc gcagagaact gtgctgcccg
      901 acgtttcttg atttcattgt ccgcccaacg gaaggcctcg gccacattgc gttttaattc
      961 ggactctaga ctgtccaatt cgtcaagata ccgcgacact ttagccatcc gctcttgcga
     1021 taatgcttga tcgtccggat ttgtcagcgc cgatgccagc aaaacctgac actggtttcc
     1081 ttcccacttg atcaagcaag cctccaccag ttggttggcc ctccaattct cgatccagga
     1141 aggcaaatgt tgatcgaaga cacacaccaa cccatgtttg cggtttcgtg tgaattcaac
     1201 atacacggcc ggccactgct cctcatcata gacgacaagg ggtcccgtcc gcgtcccacg
     1261 gtagtagttt gcaatcacat aaggatgtcc gttttcatcg aagagaaccg ctggcccgtt
     1321 gagcaatcct ttttcgaagg ccatggctgc ccgcatcaca tcgttatcgt cgtaaaacac
     1381 gtaaaaagca aaaggtgaca cgaccccggg cgtgtaatgg taccgtgttc tgcttcctcc
     1441 gatgtaagcc gcaccttcct caccgacagc accaactcca aaactaacgt cttcccgaaa
     1501 cagaccacca ccgacctctt ttttcgccga gacggtgatc tgtccgcgac ggcacacgag
     1561 aacatgtcca gactcgatgg ccaatcgcca tttctccggg ggacgcacct tccccggttg
     1621 aaagaccgcc cctgagggta gcgtgatgtc ctgacacgaa tattgctcga atgggtacaa
     1681 cgaattcgga ctcgatttcg gcgttcctcc ttgtggttga ttaacaccaa aaacacccgg
     1741 cccaatgggt gcgctttttg gtaaggccgc aaactgcggg gccgccagat gaggcagcac
     1801 ctcagtcgag gaaagggcga aattcaagtt ctgcccgaga aggttattga atgccaagga
     1861 gttgatccca acgacccgcc cctgttggtc caaaagtgga ccaccactat tgcccgggga
     1921 aattggagcc gtcgtttgaa tccaacggac ctctgctttg cgaacgggtt tgtcgaaact
     1981 tccatcatcg accacgtccc gaatttcact gcttgatcgc acggcactga cgatgcccgc
     2041 cgtgagcgtt ccggtcaaac cctgcggtgc accataggcg aagacctgct ccgcaacctg
     2101 cggaagttcc cccctgagct cgaggggctg aggcaaattg atggcctcga cggcaagaat
     2161 tgccaaatcc cgatcagcca aaacagcccc ctgctgaacg ggaatctctc gtccatcgaa
     2221 aagcttgagc ctaaggttga agttgtcccg gatgacatgg gcattcgtaa cgaccaccgg
     2281 ttttcccgca cccgtggtga caaagaagcc gctcccttgc cctcgatccc cttggatgac
     2341 gacggtagcg cgtctgactt tttccagcca acggttattc tcggccggat tggccatcgg
     2401 gttgacagcc ggctttgctt ctgcatcggg ggcacgaggt ggctccggcg gcgcctgacg
     2461 cggtaaagat tctgggggat tcgctccagg tattcgccca ggttgcggaa gaccgtgaag
     2521 cctagcaccg gcatccgcgg gggaaccatc gttcacccgt ggagcaggtg gcccaccagc
     2581 gagctgttcc tgcctcggag aagggtttcg atctccctga gaaccgatct ttttgccaga
     2641 aagccataat accagaacac ctatcacaaa gcacagtccc aagccgagac ccaacgccgc
     2701 aatccactgg tctttcgtca atcggctttt tctgcggaaa cggcggacat tccccggcat
     2761 gtaagacccc gtgccaagag gtgccgcgac ccgcggcgtt cctgagtgtc tggccggtgc
     2821 ttcttgctgg gagagaccag gcccttcgtg tgcagtactc gagagggcag catcgtaggc
     2881 ctgttttctc acggggtcga gcaggacaag cctcgcttga gtgatctcgc ttatgagctt
     2941 ctcagcgaac gcagcatggg gaccgagggc cttggtgcgc agataggcca gtcgctggtc
     3001 caccgccgct tcaatggccg tctcattcga ttcgaacggc ttcaggccaa ggaggcggta
     3061 atacgaaatc ggccgttcgg aaggtgggat tcctaaccac gtgtaatacg ggtcaaacgg
     3121 ttgagacgtc tgaggaccgc tcattgtggt gtcccccgtt cagaataaga atgatggttt
     3181 ggcttaccta ctgcccatta tccgcgccag gttggacaaa ccatgtccaa tctggaaaac
     3241 aatatcacga aaatagccct taacaacgga gaattttggc gtgctttggc ctctatatca
     3301 agcactgact gaggtttatc ccccgttggg attagtacaa aaaacaggta taattggcta
     3361 ttctgtcaac cccacggttg gttgttacaa ctcgtgttgt caacaacact tatttcccta
     3421 cccttgccgt ttgggcaacg cttggaatag tccccgttct tctggttaga catcacgatg
     3481 caaacacctg atgatgggat tgaaatcacg cgggttttgg ccgaaacatt cctggtcgat
     3541 ggcgagtatc accaaacgat tgactcgact aacaatcggg caaaagcgct ggccggttcc
     3601 ggtcagatcg gcttaccata tctcgtgctg gccgaagagc aaacagcagg ccggggacgc
     3661 gctctgagac ggtggtggac cggcagagga agtttggcat tcagcatcat ccttcccctc
     3721 actgaggaac ggccggtcaa tcggttaagc gaaaaagaaa acagtacttc acgggcgcgg
     3781 ccgaaggctt tcctttgcga agatgggatt cgcctcctcg gtttagccgg ggccttggct
     3841 gtttaccgag cggcaagtcg gcaccttccc tccgacattt ctttggggat tcactggccg
     3901 aatgacgttt acgcgaacga cagaaaactt gcgggagttt tggtggaaat aacgggcaac
     3961 cagcgagccg ttgtcggcat cggcgtcaat gtcaacaatc gaagcgagga tgccccgcct
     4021 cacgtccaac ataaggttgc cacgcttcgg gaattgtgtg gccagccaca aaatcgaacg
     4081 gagcttctta tcgacatttt gcaggagttc gagcctctct ttgagctgct caagaaccaa
     4141 cccgaaagac tggccgagga ggccaaccgt gtatgtttgc aaaaggggcg tgagctggtc
     4201 ctggaagtgg agcagggctt ggtgacgggg ctttgcctgg gcatcgacct gacgggtgcc
     4261 ttagttctca agaccgtctc gggtgaacgg cgatttttga gcggcactgt ggtcgcggtg
     4321 gaatgaagcg gccctgaccc catagaatta atggttgggc ccattgcacc gtgcaacgct
     4381 tgatggccta atcgctcgtg ttaatttcac ctccgagcgc cgctttcttt attcggaagc
     4441 taggtttatt gccagggttt ttggtgagat gaatcagggt ttagtagctc gcgagatgct
     4501 agattgggag ttcctcgaga cttaccttcg gcagagggac gcccattttg cggttcattg
     4561 ggagtggatt acagaagtcc gacataaatt ggaggcgaat gccggcctag accgcggcgc
     4621 ggtgactgac gatgtcgccc gaatgtccga acggatcgaa cgcttgcgtt atcaactgtt
     4681 gcaggagcgg agcgacccgg atgccgcaga ctaccaagcg gccgaggtgc tggattcgct
     4741 gtttgccatt ttgacagacc gcgagacaac aactcctcgt catctgcagc aggtcttgga
     4801 agatgacacc tttgccgtcg aggcaatcaa aagcctggag tggtgcttga accccgacgt
     4861 accgttggac acagtcatcg cgtcagcccg gcgattgacc aaggaacatt tcggcgcaaa
     4921 cttgcccgac caaggggccg agtccgcatc ttggccgatc tttctttatg ccccgcttta
     4981 tctctcaagc tactgtgtga accattgcct ctattgcgga tttcgttttc cggagacgac
     5041 tcctcgccgc ttgctctcgg ccgacgaggc agtggaacag gttggcctat tagttggccg
     5101 gggaatgcgg cacattttgc ttgtcgcggg cgattaccca cagaaaactg acatcgacta
     5161 ctacgtggag acaatcaccg cgatacgccg gcgctggccc gtcagcttgg ccgtggaaat
     5221 cgccccgcaa tcgacggggg gctacagaag cctggtgaaa gctggggtga tcggcgtgac
     5281 gctttaccag gaaacttacg accgaagcct ttatcgtacc tatcatatcc gcggaccaaa
     5341 ggcacactac gcctggcgat tagaaggcct agaacgggcc gctgaggccg ggatgcgtcg
     5401 tttggggctt ggaatcttgc tggggttggg ggatccggtc tccgatgcgc gggcgatggt
     5461 gcgacacgcg tattacctta aagaacggtt ccctgatcgg cgaatcgcct tcagtttacc
     5521 taggattcat gaagggcccg agggtttcca gattcccaac ccggtcaatg atgaaacgct
     5581 catccgattg tattgcggac tgcgtcacgc catgccgggc gcggagctgg tgctttccac
     5641 ccgcgagatg ccgcaacttc gtgatctcct cgccgcaacg tgtattactc aattgagcgc
     5701 gggcagctct accgttccag ggggatacga cgaaatggga acgcactttg gggacgagca
     5761 cgacgggcag ttccgcgtgg tcgatcggcg ctcggtcaac gatgtcatcg cagtgctgaa
     5821 atcccaaggg catgccatca tttggcagcc ccgggaataa aaaggcgaaa taagcgaatc
     5881 tgaataagcc gggaaatctc ggcgaacaaa gctcgcaatc agcgaggttg ccaaatggcc
     5941 tatcgacact gtaagctaac tacctaagag tattgcgtaa cagagaacaa cattgcccca
     6001 ccttcccgag atttcggcat gaaaacaaca gacctgttgt tgcccggcta tatggcgctg
     6061 tagcacttat tgctagagtg cgtcgtttgc gcagcgggtc gtgctacact taaagacggg
     6121 tggtctgcca ccaggaacgg tgggtaggag tcatcgaatc acggaatcgg actaaaagga
     6181 gtgaaggagt gtttgttgcc gcaacaacgc gttgctttgc caatcttcca ttagaagcgg
     6241 ctttcgagcg actcaatgac ctggagtttg gatacgtcga gatcatgctc cacgagtccg
     6301 acggacacct caagccgtcg gaagtggccg cagatttaga tcgggctatt gctctttgcc
     6361 ggaagacgta tcggctgacg ccgattgctt acagtgttga tatcgaagcc caaggagacg
     6421 agtattaccg gcagtttgcc gcctgctgta agttggccaa ggccacccgc gttgtcacgg
     6481 tcgtggtacg tgcttccgaa ttagggacac ctttcaacga ggaggttgaa cgccttcaga
     6541 atcttgtcgc catcgccacc atggaagagg tcgtcgtggc attgttgacc gagcgtggcc
     6601 gcatgagcga attgcccgaa acggcggccg tcttgtgtca tcacgccaaa ggtttgggaa
     6661 tttgcctcga ccccagccat cttatctacg gccgagagac gccgctcaac cccgaaccgg
     6721 tcctcaagta cgttcgacac gtcaggctgc gggatacgag aaaagataag ttccaagttc
     6781 tggtgggcca gggtgaagtg gaatacggga agctcttggc caatctgggg cgata
//
LOCUS       DAOSUT010000195         6835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5387_length_6835_cov_9.835251, whole genome shotgun sequence.
ACCESSION   DAOSUT010000195 DAOSUT010000000
VERSION     DAOSUT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_6835_cov_9.835251"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1183)
                     /locus_tag="PLD05_13905"
     CDS             complement(209..1183)
                     /locus_tag="PLD05_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78583.1"
                     /translation="MPDCSPAQLIGYLLNALDEEEREEIEQELVRCPELRKKLQRLKR
                     RLAVLELGRREIAPPPGLADRTCQFVFQSCGCLAIRHTTKATSPALPASRDVAGFRLS
                     PLSDYLGGHGANWSFADLVVSALVVLIVVSLLFPALAEVRFRSRVLACRENYRELYQA
                     VHQYQNVNALAGAAGIAQANFAVAKQFLTPPLSWSAIRRCPGMVVESSVNDGSRSVKV
                     SESWVESETAFDREFGGVLLGYSPVIHTASLGSAIPYEQVSLAAIPLWRDSPHPRDCQ
                     GWSWNHEGRGENWLFADGHVAFLPRHRTNERPTVLQAVISSVSFTNSP"
     gene            complement(1176..1826)
                     /locus_tag="PLD05_13910"
     CDS             complement(1176..1826)
                     /locus_tag="PLD05_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP78584.1"
                     /translation="MDTAPRLEWLNHGAPRAEWSDEDLVREYQQTGEPRAFEELVRRY
                     ERELYNYLCQYLGDAEMAEDAFQATFLQVHRKCSQFDSGRRFRPWLYAVATNQAIDAQ
                     RRNRRHRLVSLDQQSRMYTESHDLGCLIDLVEGQEMDPEEHVELNERKEDVRQAVDQL
                     PESLRQVLLLVYYQGMKYREAAEVLNIPVGTVKSRLHTAIRRLEYHLKLPRSPSHA"
     gene            2369..3748
                     /locus_tag="PLD05_13915"
     CDS             2369..3748
                     /locus_tag="PLD05_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78585.1"
                     /translation="MGQRNVDGPRSQRLGRRRFLQTSVLAATASGVGVPEIIPRRAMA
                     WEGQPGANERITVAHIGMGGRGKGLYREMAGLRAAGLCDHVAVCDIDEKRLEEASRLV
                     GPQADVYRDYRYILERKDVDAVVIATPDHWHAVQFVHAAECGKHIYCEKPACCTIEEG
                     KAMVAAARKAKIASQIGSQGRSQPEAYLAHRYLVNGVIGRVYRVDCWHYPSPVDTSHT
                     PDSDPPPELDWDLWLGPLRWRPFNKRYLHGVFRWLLESGGGQIRDRGAHVMSCAMWWM
                     GADGTGPVEVEATGTPPEEGLWDSCVEMNVTYTFKNPDWILTWNQPGEPVPPEERTGQ
                     EPGGKIVRPGYGAVYRGERGTMVHWGGDGGTWVEKKVREWVPPPGAKDVYKSPGHFED
                     WFEGIKTGKKTIMDVEAGVGVAFLCILGNLSYMLGRKLHWDPVKQEIIGDEQARRLMV
                     RPQRHPYHL"
     gene            3835..4413
                     /locus_tag="PLD05_13920"
     CDS             3835..4413
                     /locus_tag="PLD05_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78586.1"
                     /translation="MEPLTLEKLLELLKSDDPLVRRDAWLKAGPLGGDALPSLADLAA
                     KADLEISRAANRAMWQIVRYAGAPGRETERQGVVEALGELVVDTRLPVQLRRDVVWML
                     SEIIADEEIDESIASSLLKDPELQEDVRMALQRVAGPKAVALLKAALNQAEGDFKAAL
                     ACSLRKCHVAIDDPPCPKLVPQKQTRVKPVGR"
     assembly_gap    4738..4837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4838..5350
                     /locus_tag="PLD05_13925"
     CDS             <4838..5350
                     /locus_tag="PLD05_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78587.1"
                     /translation="SLGPCTDIAAAWLKEPRIKDRVIAFWHGRTEWPTKAWNFNAYND
                     LKAVRILFSSDLPFILFDTGTYLTCPMEESEKRIRPHGALGAYLHEIRFRSPAWQSPN
                     KGLFDLGDIAALVDPSLVQFEVVNAPSVQWDMLYDHKNTHGRMVRIFSIDRDKTFELL
                     EQRLARAATQ"
     gene            complement(5400..6590)
                     /locus_tag="PLD05_13930"
     CDS             complement(5400..6590)
                     /locus_tag="PLD05_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOP78588.1"
                     /translation="MRRLTAYPFLLVILLCVILGDGPVGTIAPRDAFAQQSPGRTLLV
                     AVDRYGPLDEQTRAELRRQLEETGKVLEAQSAAMRLVAKLVGPAVVHIEAQTIGRRAL
                     QHGAARPVEEAGSGVLVKLGEKTCVLTNRHVIAGATPDKVRISLADGRVIQPQQIWSD
                     TESDVAVLEVGISDVVTAILGNSDTVDIGDFVLAFGSPFGLSHSVTFGIISGKGRRDL
                     DLGDSGVKFQDFLQTDAAINPGNSGGPLVNLKGEVIGINTAIASSSGGNEGVGFAIPI
                     NTFLFTARQLVEKGAVTRAFLGVTLDARFTIEAAQAAGLQQRIGARVVTVIPDSPAAQ
                     AGIQAGDIILRYDTTLVESDGHLINLVNTSDVGKEVTLEIVRAGQVQKVKVVLTPKPA
                     TPAN"
BASE COUNT         1454 a         1919 c         1911 g         1451 t
ORIGIN      
        1 agctaagagc ctatcccata atctcccctc gcccgtttgg cgggagaggg gtcggggttg
       61 agggtggctg gaatcgctgc cggttttctc cgtcgattcc gacgtccgct tgccctcacc
      121 ccaaccctct cccggaggga gagggggact tcttgggaaa ggctttaagg ggaaccctct
      181 cgcggtgcag aataatttcg gcaattggct agggagagtt cgtaaagctg accgaggaga
      241 tgaccgcctg caataccgtg ggccgctcgt tggttcgatg tcgcggcaaa aacgccacgt
      301 ggccatctgc aaagagccag ttttctccac gaccttcgtg attccaactc caaccctggc
      361 agtcacgggg atgaggactg tcccgccata acggtatggc cgctaaagac acttgctcat
      421 aagggatcgc cgaaccaaga gaagccgtat ggatcaccgg cgagtagccg agcagtacgc
      481 cgccaaactc tctatcgaaa gcggtctccg attccaccca agactcgctc acttttacgg
      541 aacggctgcc atcgttaacg ctggattcga ccaccattcc cggacagcgt cggatagcgg
      601 accaagacaa gggcggtgtc aggaactgtt ttgccacagc gaagtttgcc tgggcgatac
      661 cagcagctcc tgccaaggca ttaacatttt gatactgatg aacggcttga tacagttccc
      721 gatagttctc acgacatgcg aggaccctcg agcggaaacg aacttctgcc aaggctggga
      781 aaaggaggct caccacgatt aggacaacca acgcgctcac cacaaggtcg gcgaacgacc
      841 aattagcccc atgacccccg agatagtcgc tgagagggct aagccggaag cccgcaacat
      901 ccctactggc cggcaaggcg gggcttgtcg ccttcgttgt atgtcgtatg gctaaacacc
      961 cacagctttg aaaaacaaac tggcaagttc ggtcagctaa tcccggcggg ggggcaatct
     1021 cccttcgccc aagctcgagg acagccaatc gccgcttcaa tcgctgaagt tttttgcgta
     1081 gctccgggca tcgcaccagt tcttgctcaa tttcctcccg ctcttcttca tccagagcgt
     1141 tcaagaggta cccaatgagt tgggctggtg agcaatcagg catggctcgg tgaccgcgga
     1201 agcttcaagt gatattccag tcggcgtatt gccgtgtgca aacggctctt gacggttccc
     1261 acagggatat tcaaaacctc ggcggcctcg cggtacttca ttccttggta gtacaccaaa
     1321 agaaggacct gccgaaggct ttccggtaac tggtccaccg cttggcggac atcttcttta
     1381 cgctcgttga gttcaacatg ttcctcggga tccatctcct gcccctcgac aaggtcaatc
     1441 aaacacccca gatcgtggct ttctgtgtac atccgtgatt gctgatcgag actgaccagg
     1501 cgatgtcgcc ggttccgacg ttgggcgtcg atggcctggt tcgtagccac ggcatacaac
     1561 caggggcgaa aacgccgtcc cgaatcaaac tgagagcact tccggtggac ctgaaggaag
     1621 gtggcctgga aggcgtcttc agccatttcc gcatcgccta aatattgaca cagatagttg
     1681 tacaattccc gttcataacg gcggacaagc tcttcaaatg cgcggggttc ccccgtctgc
     1741 tggtactcgc gaacgaggtc ctcgtcggac cattcggccc gcggagcgcc gtgatttaac
     1801 cattccagac gcggggctgt atccatcgaa aagactctcc tagcgaactt cgctttgatt
     1861 cggtgccgac gggaagacgg ctgtttgctc ttgcgaagcc tccggataca aatcattgta
     1921 atcactcctc gcgttgattg ggtcgttgcg gagggttgaa aaacaggcgg gaacttcggc
     1981 cgtcatcttg tagcaaacac cacgccaaac gattctcgct cgaagaagtt tcttccttgg
     2041 acacacagta tgcatttacg tctattgtgg cattttttaa tttgctcttt tattgtccag
     2101 attgccattt tttggtgtga ggctacccgt ttagccgcgg ggcgacgcca ggctgatccg
     2161 cggatttccg cgcgttccgc cggtggtcgc gggctgaggc aattccgcgg gggaaatcct
     2221 cagaatctgt gaaaagcttg tatcctcgtg cgctgcgggt tattctggct atgacacaaa
     2281 cggttggtga cggcgtatcg gccggcctgg gggagttacc ggtccggtat tgaacgcagt
     2341 aatggcatca cttcgtggag gaatcgtaat gggtcaacga aatgttgacg gcccgcgtag
     2401 ccagcgactg ggccgacgtc ggtttttgca aacgagtgtc ttggcggcaa cggcctcagg
     2461 agtcggagtg ccggagatca tcccccgtcg tgcgatggcg tgggaagggc agcccggcgc
     2521 caacgaaagg atcacggttg cccatattgg catgggtggg cggggaaaag gtctctaccg
     2581 agagatggcg ggtctgcgtg cggcagggct gtgcgaccac gtggccgttt gtgacattga
     2641 cgagaaacgt ctcgaggagg cctctcgcct tgttggtccg caggcggacg tctatcgcga
     2701 ttaccgttac attttggaac gaaaagatgt cgatgcggtg gtgattgcaa caccggatca
     2761 ctggcacgcg gtccaattcg tccacgcggc tgaatgtggt aagcatatct actgcgaaaa
     2821 gccagcctgc tgcacgattg aagagggcaa ggccatggtg gcggcggctc gcaaagccaa
     2881 gatcgccagc cagattggct cccaggggcg gtctcaaccg gaggcctatc tcgcccatcg
     2941 gtatctggtg aacggcgtca ttggtcgcgt atatcgtgtt gactgctggc actatcccag
     3001 cccagttgat acaagccaca ctccggatag tgacccaccc ccggaacttg actgggacct
     3061 ctggcttggt cccctgcgtt ggcgaccctt caacaagcgg tatttgcacg gcgtgtttcg
     3121 gtggcttttg gagtcaggcg ggggacaaat ccgggaccgc ggtgcccatg ttatgagttg
     3181 tgccatgtgg tggatgggcg ctgacggaac aggacctgtg gaagtggagg ctaccggcac
     3241 gccacctgaa gaaggtcttt gggattcgtg tgtggaaatg aacgtgacct acacgtttaa
     3301 gaatcccgat tggattctta cgtggaatca gccgggcgaa cctgtaccgc cggaagaacg
     3361 gacgggtcaa gagccaggcg gtaaaattgt ccgtcccggt tacggggccg tgtatcgcgg
     3421 ggaacgcggg acaatggtcc attggggcgg cgacggcgga acctgggtgg aaaagaaggt
     3481 ccgcgaatgg gtcccaccgc ccggggccaa ggacgtgtat aaaagccctg gtcacttcga
     3541 agactggttt gagggtatca agaccggcaa gaagaccatc atggatgtcg aggccggggt
     3601 tggcgtggcc tttttgtgta tcctcggaaa cctctcttac atgctggggc gaaaactcca
     3661 ctgggacccg gtcaaacagg agattatcgg cgatgaacag gcccgccggc tcatggttcg
     3721 gccacagcgg catccttatc acttgtaatg tgacggaggc tttttgactc ttggccattg
     3781 agtgcgaggt cattttcgaa atcagacacg tttgcgcata taaaggatac agctatggaa
     3841 cccttaaccc tggaaaaact ccttgaattg ctgaagtcgg atgatccctt ggttcgccgg
     3901 gatgcatggc tcaaggccgg gccattgggt ggtgatgctt tgccgagcct ggccgatctc
     3961 gcggcaaagg cggacctgga aatcagccgt gccgccaacc gtgccatgtg gcagattgtc
     4021 cgctatgccg gggcccccgg tcgggaaacg gaacgacagg gtgtcgtaga ggcactcggc
     4081 gaattggttg tcgatacccg gctccccgtg cagcttcggc gcgacgtggt ctggatgctc
     4141 tcggagatca ttgcggacga agagatcgac gaatcgatcg cctcctcatt actgaaggat
     4201 ccggaactgc aagaagacgt ccgcatggcc ttgcagcgtg ttgccggtcc caaggccgtt
     4261 gccttgctca aggccgccct aaatcaggcc gaaggcgact tcaaggctgc cctcgcgtgc
     4321 tcactccgca agtgtcacgt ggccatcgat gatccaccct gcccgaagct ggtgcctcaa
     4381 aagcaaactc gggtcaagcc ggtcggtcgg tgaatggatt gaccagcggc atgggttgtt
     4441 cccggtcgag aatggtcttt ggggcttcgc caaacagaat aggacttaaa tccaccgagg
     4501 gtgttaatcg aaacccttga gaggcgcggt gggcgttttc aaaacgaaga attttgactt
     4561 cccaacaatc gacacgagga atccctcaag gagcgacttc atggtttgga gttcctttcg
     4621 tggttggatc ctggtatgcc ttgtcgtctg caaccaagcc ggcctgccct cgacctaagg
     4681 gcaagtgaat cgtcaaacgc gcgcgttgtg ccaccgtggc cgccgacgga gacccgcnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg ttaggtccct gcaccgacat
     4861 cgccgcggct tggctcaagg agccacgcat caaagatcgt gtcatcgcct tctggcacgg
     4921 acggaccgag tggccgacca aggcctggaa tttcaacgcc tataacgacc ttaaagccgt
     4981 gcggattctc ttttcgtccg atctgccgtt cattcttttc gacacgggca catatctcac
     5041 gtgtcccatg gaagaatcag aaaaacgtat tcgaccgcac ggcgccttgg gcgcgtacct
     5101 gcacgaaatc cgttttcgct cgccggcctg gcaatcgccc aacaaaggtc tttttgactt
     5161 gggtgacatc gcagccctgg tcgatccctc ccttgtgcag ttcgaagtcg taaatgcgcc
     5221 aagcgtccaa tgggacatgt tatacgatca caagaacacc cacggtcgaa tggtccgaat
     5281 tttttccata gaccgcgata agacgtttga acttttggaa cagcgactgg caagagcggc
     5341 aacacagtaa tccggcccgg aattgggcaa tgcatctgtc atagctgacc gttaagcgat
     5401 cagttggcgg gggtggccgg ctttggggtg aggacaacct tgaccttttg gacctgcccc
     5461 gcccgcacga tctccagcgt tacctctttg cccacgtcag aggtgttaac caagttgata
     5521 aggtgaccgt cactctctac gagcgtcgta tcgtagcgga ggatgatatc gcccgcttga
     5581 atgccggcct gcgcggcagg cgaatcgggg atcaccgtca caacgcgggc accaatccgt
     5641 tgctgaagtc ccgctgcctg ggctgcttcg atagtaaaac gcgcatcgag cgtcacccca
     5701 agaaacgccc gcgttacagc ccctttctcc accaactgcc ttgccgtgaa aaggaatgtg
     5761 ttgatgggaa tggcaaaacc aacgccctca tttccgcccg aactactggc aatggccgtg
     5821 ttaatgccga tcacttcgcc cttcaaattg accagtggcc cgccactgtt ccccgggtta
     5881 atagcggcat cagtctgcaa aaagtcttga aatttcacgc ccgagtcccc aaggtcgaga
     5941 tctcttcgcc ccttaccgct gataatccca aaggtcaccg aatggctcag accaaaaggg
     6001 cttccaaacg ccaaaacgaa gtcaccgatg tcaacggtgt cgctattccc caggattgcc
     6061 gtgacgacgt cagaaatccc gacttccaac acggccacgt cagattccgt atccgaccaa
     6121 atttgctggg gctgaatcac ccttccgtcg gccaatgaga tccgcacctt atccggagta
     6181 gccccggcaa tgacgtgacg attggtcagg acacacgttt tttcaccgag cttgaccaag
     6241 acgccggaac cggcttcctc gaccggtcgg gctgcgccat gctgcaaggc ccgccgcccg
     6301 atcgtttggg cctcaatgtg caccacagcc ggaccgacca gcttggccac cagtcgcatc
     6361 gctgccgatt gggcctcgag aacttttccg gtttcttcca attgccgccg caactcggcc
     6421 cgcgtttgct catccaaagg accgtaacga tccaccgcta ccaggagcgt ccgccccgga
     6481 ctctgttggg caaacgcgtc ccgcggcgca atcgtaccaa ctggcccgtc cccaaggatg
     6541 acacacaaaa ggataacgag cagaaatggg taagccgtta atcgtctcaa atgggttctc
     6601 atgatcatgg ggattaaatg tttcttatgc cgatcgatgc catacggtgc ccccttttcc
     6661 ctttaaacaa cctagccccg agaaggcccc ctttttatcg ggatgatgat tccgaaccgg
     6721 ttggtccaat catcggttgg gcccatgagc taaactctgc gcggcgcccg ttgaatgggc
     6781 tccctcaatt atagcccacc cacggccagc acctaaacgt gccgaaagtt tgtct
//
LOCUS       DAOSUT010000196         6793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5421_length_6793_cov_9.722024, whole genome shotgun sequence.
ACCESSION   DAOSUT010000196 DAOSUT010000000
VERSION     DAOSUT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5421_length_6793_cov_9.722024"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLD05_13935"
     CDS             complement(<1..1147)
                     /locus_tag="PLD05_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78589.1"
                     /translation="MSTIFSATILIAFGLWAANSEQQTVIRNGDWVVTDHETVADQHI
                     VLNGSLILEKGGQLTLRNCHLEIIGEKSREHIVDWRGGKLVTLKTVLGGTDRTGTAVH
                     TVFHLYDGIWEAEDTTVEFSYGISFSDTARGILRAKRLRAGRRPDAVIASGLADITLE
                     DCVFPVALGIYTHQGGDVTLALPSKRPLDASFDSTNLTPGVKWKLTLVRTEVPQWFVF
                     LRNIGPSNPPCVVTLQEADDTIISLLGHNLAGDIFLTPNLSEPLRLGSLTLKRGSAEI
                     RVPMWALYFSGDKTDVNIRGKTHICELMVRAGKVRFAGDNLSELSIGCTTLELSGNAY
                     LELKNVHLGRPLSWRGESSEMGEINAVDNAVLMAENVTMRNLVLHAKD"
     gene            complement(1244..2179)
                     /locus_tag="PLD05_13940"
     CDS             complement(1244..2179)
                     /locus_tag="PLD05_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOP78590.1"
                     /translation="MARPLSKLAKDWWDYTTLDPELLQDAAKLTVRQVERLSRPGFKV
                     VMYDTLEEFYLAEALEYIHAWKQSTADNPCGICGPIGPTEQLPLVARLVNELELDVRD
                     GHFWGMDEWVDELTGEPVPMDHPLSFARADMELCFKRINPRLRMPKEHIRFPIGDLEA
                     YSRSFDEIRCVIMQGGQGDVKHWAFNDPPKRQGRWKDAPPPPSEYRKLKTRITDLHPA
                     TIMQNARTSGGGQVHLVPTKAATVGPVETWKAEKVSIWHAGMHDNPFGMRLTALMISK
                     GIADSAVPMSLLADHPNVQFNFYRPALAEVKTEMH"
     gene            2435..3340
                     /gene="truB"
                     /locus_tag="PLD05_13945"
     CDS             2435..3340
                     /gene="truB"
                     /locus_tag="PLD05_13945"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOP78591.1"
                     /translation="MEGHTRTICGVFCLNKPAGITSRDAVNLVQRSLRRVRLGHAGTL
                     DPLATGVLVVCAGLATRLIPFIQELPKLYRATFLLGRSSPTDDVDGEVTVFEDAPVVT
                     REQVESTLPEFVGTILQSPPVYSAVKIEGRRAYEWARKGAPREPKPRPVEIHSLKLLR
                     YEYPELELEIHCGSGVYVRSIGRDLAQRLGTAAVMSRLTRLAIGHFVIDQAVDPHEIG
                     PHNWSEYLMPPAVAVKHLRQIQLSEDHIAQLVHGQWPAYDGDLAAGQVAAVVDQLGNL
                     VSVVECQRNKRLRPLVNLPRFSADP"
     gene            3471..>4525
                     /gene="gcvT"
                     /locus_tag="PLD05_13950"
     CDS             3471..>4525
                     /gene="gcvT"
                     /locus_tag="PLD05_13950"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOP78592.1"
                     /translation="MTDLKHTPLAGWHKENKAKMTPFAGWEMPLHYQSGILQEHLTVR
                     RYGGLFDISHMGRFRIAGPDTVRFLQYVLTSDVSKLGPWRAQYTILAKEDGSAIDDAY
                     LFRFGDEYWLVVNAANSAVDWQHLSEVARGFSVQLEDLTAEVAMFAVQGPESETLLRQ
                     LLQDGALPDEGRNTLSSGTLAGVPVKLSRTGYTGEPIGFEVFVPAKAALALWESLVHA
                     GASLGLAPIGLGARDTLRLEAGLTLFGHELGTTPQGQPIPILAAPSAIVAVSLAEEKG
                     DFLGRKSLAAQVKCYRQWREGHAQKNPILPQIVRPVAILDAGVARQGDNVFWKGTPVG
                     QVTSGTMVPYWVFEGDDS"
     assembly_gap    4526..4625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4626..6008
                     /locus_tag="PLD05_13955"
     CDS             <4626..6008
                     /locus_tag="PLD05_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78593.1"
                     /translation="SVREQVSTLLERSLENHLWRQQRCINLIPSEMTPSPLVRLLQVT
                     DPVGRYAEHNYLTQLGREVYYYQGTHFIEWVEQQLIDEFGRYLGTDQIEPRLISGQTA
                     NMAVFSAVVAFRNRDNKLGEIERIRGVLNHHLNLGGHLSAQAMGPLRDYVARDPTTGR
                     FMIKHFPVCADNPYRVDVEATAALLDDWAPELVIFGKSMFLCREPIAEIRQILAERNR
                     RPLIMYDAAHVLGILGPVFQDPLAEGADIVTGSTHKTFFGTQRGIIASALNSDSPFAE
                     LWDHIRRRAFPGFLSNHHLGTLLGLLLAVMEMNVFRQEYQSQVIKNAKAFARALRECN
                     LQVEGDPAMDFTETHQVLLRVGSHRGIEAAQLLEENNIIVNYQALPGDENFAASSGLR
                     MGVAEMTRFGMKEADFQELASFIADILLHKKQLGEKVSQFRGRFLKMHYCFDPADWPD
                     LTQKLLGTFS"
     gene            complement(6099..6629)
                     /locus_tag="PLD05_13960"
     CDS             complement(6099..6629)
                     /locus_tag="PLD05_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOP78594.1"
                     /translation="MDVFEAINKRYSYRGPFRDVPVPREHLRKIVEAGIRAPSGKNAQ
                     TTSFVIVDDPVLIRQIAALFENKPVCSTAKAMIVCVTDPRPVMGDVSFHVEDCAAAVE
                     NILLAITALGYATVWIDGALRYNRIAERIGELLGIPSDRIVRVLLPVGVPAEPGTQRE
                     KLPFEQRAWFNGWKKS"
BASE COUNT         1461 a         1880 c         1830 g         1522 t
ORIGIN      
        1 gatccttggc atggaggacg agattccgca tcgtgacatt ctcggccatc agaacagcgt
       61 tatccactgc attgatttca cccatttcgc tcgattcacc tcgccagctc aatggtcgcc
      121 caaggtgaac attcttgagt tcaagatagg catttccaga caattccagc gttgtgcacc
      181 caatgctcag ctcgctaagg ttgtcgcccg caaagcggac ctttcccgcg cgcaccatca
      241 gttcgcagat atgcgttttt ccgcggatgt tcacgtcggt tttgtcccca gaaaaataaa
      301 gcgcccacat gggcactctg atttctgccg agccccgttt taatgtaagc gaaccgaggc
      361 gcagtggttc cgacaagttc ggtgtaagaa aaatatctcc cgcaagattg tgccccaaca
      421 aagagatgat cgtatcatcg gcctcttgca aagtgacgac acaaggcggg ttactagggc
      481 caatatttcg caggaacaca aaccattggg gcacttccgt tcgcaccaaa gtcaatttcc
      541 atttcacgcc cggagtcagg tttgtgctgt cgaaagaagc gtcaaggggc cgtttcgacg
      601 gtaatgcgag ggtaacatcc cctccttgat gcgtataaat gcctagcgcc accggaaaga
      661 cgcaatcctc cagcgtgatg tcggccaagc ctgaggcaat cacggcatct ggtcgccgcc
      721 ctgcccgcaa tcgtttcgcg cgaagaattc cccgggcagt atctgagaag ctgatgccgt
      781 aggaaaactc cacggtcgta tcctcggctt cccaaatgcc gtcgtacaaa tggaacacgg
      841 tgtgcacggc tgttcccgta cgatccgtgc caccgaggac ggtcttcaag gttacgagtt
      901 ttccacctcg ccaatcaacg atatgctccc gagacttttc gccaatgatc tcgaggtggc
      961 agttcctcaa cgttaactgt ccaccctttt cgagaatcaa actgccgttc aacacaatgt
     1021 gctgatcggc cacagtctca tggtctgtga caacccaatc accgttgcga ataacggttt
     1081 gctgctcaga attcgcagcc caaagaccga atgctattag aattgtggca gaaaaaattg
     1141 tgctcatcgt cgttcgacac cttgaccctc aacattttgt gcaaaatacc tcccgcctcg
     1201 ggtagcccga tgttttgcgt gatagatccg cgagccgagc acctcagtgc atctcggttt
     1261 ttacttccgc caaggccgga cggtaaaagt tgaactggac attgggatgg tcagccaata
     1321 acgacatagg cacggcagag tcggcaatcc ccttggagat catcagggcc gtgagccgca
     1381 tcccgaaggg gttgtcatgc atccccgcgt gccagatgga gaccttttcg gccttccacg
     1441 tttccaccgg tcccacggtg gccgccttgg tgggcacgag atggacttga ccacctcccg
     1501 acgtccgcgc attttgcata atcgtggcag gatgcaaatc agtgattcgt gtcttcaatt
     1561 tccggtattc tgaaggcggt ggcggggcgt ccttccaacg gccttgacgc ttgggcggat
     1621 cgttgaacgc ccagtgtttg acatccccct gtcccccctg catgatcacg cagcgaatct
     1681 cgtcgaaact tcgcgaatac gcctccaggt ccccgatcgg aaagcggata tgctccttgg
     1741 gcatccgcaa ccggggattg attcgcttga aacacaactc catatcggcc cgggcaaagc
     1801 tcaaaggatg atccatcggg accggctccc ctgtgagttc atcaacccac tcgtccatac
     1861 cccagaagtg gccatcgcgc acgtcaagct caagttcatt gaccaagcgg gcgaccagtg
     1921 gcagttgttc tgtcggtcca atcgggccac agatgccgca ggggttatca gcggtggact
     1981 gcttccaggc gtgaatatac tcaagcgctt ctgccaaata gaattcttcc aaggtgtcat
     2041 acataacaac cttgaagcca ggtcgcgaaa ggcgttcgac ctgccgcacc gtcaactttg
     2101 ccgcatcttg gagcagctcc ggatcgagcg tcgtgtaatc ccaccagtct ttcgccaatt
     2161 tgctaagagg ccttgccatg atgcactcct ttcgagaaga gtttcgacca tcttcgtgcc
     2221 ggacgatttt tagccccatt atcaggaaaa ttctcttccc gtcaatgacg tgccgtcgtt
     2281 tgccagggca caaccgccaa gccgggggca ttatcgctcc ggaaaaatct cctatcatga
     2341 aactttggaa cgagcatatt cccctcggga gaatcttgtt ttttcgaact ttcctcggct
     2401 gttttcactc ttgcatggtt aatgccgttt gacgatggaa ggacacactc gcacgatttg
     2461 tggagttttt tgcctcaaca agccggcggg catcacatct cgcgacgcag tgaacctcgt
     2521 gcagcgatct ttacgccggg tgcgattggg tcatgccggc acacttgatc ctcttgctac
     2581 aggggtgttg gtcgtctgtg cgggcttggc gacgcgtttg atccccttta tccaggaatt
     2641 gcccaagctg tatcgcgcta cattcttgct ggggcgatcg agtcccactg acgacgtcga
     2701 cggggaggtc accgttttcg aagatgcccc cgttgttacg cgtgaacaag tggaatccac
     2761 gctgcctgaa ttcgtcggga cgattcttca aagcccaccc gtctattccg ccgtcaagat
     2821 cgaaggccga cgagcctacg aatgggcccg aaaaggtgcc ccccgcgaac caaaaccacg
     2881 accggtcgag attcatagct tgaagctcct ccggtacgag tatccggagt tggaattgga
     2941 gatccactgc ggctcgggcg tgtatgtccg gtcgattggg cgggatttgg ctcagcggtt
     3001 ggggacggcg gcggtcatgt cgcggctaac gcggttggcc atcgggcatt ttgtgatcga
     3061 ccaggcggtt gatccgcatg agattggtcc ccacaattgg tcggaatatc ttatgccgcc
     3121 tgccgtggcc gtaaagcatc ttcgccagat tcaactttcc gaggaccata tcgcacaact
     3181 cgttcacggc caatggccag cgtacgatgg cgatttggcc gccgggcagg tggcagccgt
     3241 ggttgatcag ctcggcaatc tggtaagtgt ggtcgaatgc caacggaata agcggttaag
     3301 accccttgtc aatttgcccc gcttttcagc cgatccctaa ccacagtccg tcactcagcc
     3361 attatgatga agaaatactt tagcggtgga aatgacgaca ccgcggtaag acggaagggt
     3421 tttcggaacg gacatcgcgg aacagtttcg aggaaaaagg aagtatcaag gtgaccgatt
     3481 tgaaacacac ccccttggcg ggctggcaca aagaaaacaa agccaaaatg acgccgtttg
     3541 ccggctggga aatgccgttg cactaccagt caggtatcct tcaggaacat ttaacggtgc
     3601 gacgctatgg cggccttttt gacatcagcc acatggggcg gtttcgcatc gcagggcctg
     3661 acacggtgcg ttttcttcag tacgttctta ccagtgatgt cagcaagctg ggcccgtggc
     3721 gggcacaata taccatactg gcaaaagaag atggctcggc catcgacgat gcctacctct
     3781 ttcgctttgg cgatgagtat tggctcgtcg tcaatgcggc gaacagtgcg gtggattggc
     3841 agcatctcag cgaggtggcc cgggggtttt ccgtgcagtt ggaagaccta actgcggaag
     3901 tggcgatgtt cgcggtgcaa ggaccggaga gtgaaacgct cctgcgtcaa ttacttcaag
     3961 acggagcctt gcccgacgag ggccgtaaca ccctttcatc gggaacgttg gctggtgtcc
     4021 ccgtcaaatt atctcgaact ggctacaccg gggaaccgat tgggtttgaa gtatttgtcc
     4081 ccgcaaaggc agccctcgcg ctatgggaat cgctcgtcca tgccggggcg tcgctagggc
     4141 ttgctcccat cggtcttgga gcccgcgata cgctgcgact ggaagcgggg ttgacgctgt
     4201 ttggacacga actggggact actccacaag gtcagcccat ccccattttg gcggcaccgt
     4261 ctgcgatcgt tgccgtctcg ttggctgagg aaaagggtga ctttctaggc cggaagagcc
     4321 tagcagccca ggtgaaatgc tatcgccagt ggcgcgaggg ccatgctcaa aaaaacccca
     4381 tcttgcccca gatcgttcgg cccgtcgcca tccttgatgc cggcgttgct cgacagggag
     4441 ataacgtttt ctggaaagga acgcctgtgg gccaggtcac aagtggtaca atggttcctt
     4501 attgggtttt cgagggagac gactcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnagtgt ccgggaacaa gtcagcacgc ttctggagcg ctctttggaa aaccatctgt
     4681 ggcgtcagca gcggtgcatc aatctgatcc cttcggagat gacgccttcg ccgctggtgc
     4741 gattgctcca ggtcaccgat cccgtgggcc gatacgcgga gcacaattat ctaacccagc
     4801 tcggccgcga ggtctattac tatcagggaa cccatttcat cgaatgggtt gaacagcaac
     4861 tgatcgatga gtttggacgt tacctgggca cggaccagat cgagccgcgc ctcatcagcg
     4921 gccaaacggc gaacatggcc gtatttagcg ccgttgtggc ctttcgcaat cgggataaca
     4981 aactggggga aattgaacga attcgcgggg tactcaatca ccatctcaac ttgggcggac
     5041 accttagcgc ccaggctatg ggaccattgc gagactacgt ggcgagagat ccgaccacgg
     5101 gacgcttcat gatcaaacat ttccctgttt gtgcggacaa cccataccgc gttgacgtgg
     5161 aggccacggc ggctctgctg gacgattggg cgccggaatt ggtcatcttc ggcaagagca
     5221 tgtttctgtg tcgggaaccg attgccgaga ttcggcaaat tctggccgag cgaaatcggc
     5281 ggcctctcat tatgtacgat gcggcgcatg tcttgggtat tcttggcccc gtgtttcaag
     5341 accctctggc cgaaggggcc gacattgtga caggttccac ccataagacg ttcttcggga
     5401 cgcagcgagg aatcatcgcg tctgctctga actctgattc accatttgcc gagctttggg
     5461 accacattcg ccgccgtgcg tttccgggat ttctcagtaa tcaccacctc ggaacgctcc
     5521 tcggcttgct tttggccgtc atggaaatga atgtctttcg ccaagagtat caatcgcagg
     5581 tgatcaagaa cgccaaggcc tttgcgcggg cactgcggga atgcaatctc caagtggaag
     5641 gcgatcccgc catggacttt accgaaacgc atcaggtgct gctgcgggtg gggtcacacc
     5701 gcggcatcga agcggcccaa ctgctggagg agaataatat catcgtgaat taccaggccc
     5761 tcccgggtga tgaaaatttt gccgccagtt ctggcctgcg gatgggcgtt gccgagatga
     5821 cccgctttgg catgaaggag gccgacttcc aagagcttgc cagcttcatc gccgacattt
     5881 tgcttcacaa aaaacaactc ggcgagaagg tgagccaatt ccgtggccgc ttcctgaaga
     5941 tgcactattg ctttgacccg gcagattggc cagacttgac ccaaaagcta ctgggcacgt
     6001 tttcgtgaaa acttcggaat cgcgcaatac ttcagcgtaa tggtggccgc catggggctg
     6061 ccgtctgatc gtgggcgcga ttgtatctcc aggaccactt acgacttttt ccagccgttg
     6121 aaccaggccc gttgttcgaa aggtaacttt tcgcgttgag tgccaggttc tgctgggacc
     6181 cccaccggca ataagacgcg aacaatccgg tcggatggaa tcccaagcaa ctcgccaatc
     6241 cgttcggcaa ttcgattgta acggagcgcc ccatcgatcc acaccgtggc atagcccaag
     6301 gctgtgatgg caagcaggat gttttccacg gcggcggcac agtcttccac gtggaaggag
     6361 acgtcgccca tcaccggtcg cgggtccgtg acacacacga tcatcgcctt ggccgtgctg
     6421 caaaccggct tattctcgaa aagtgcggca atctgtctaa tcaatacggg atcatccaca
     6481 atcacgaaac ttgtcgtttg ggcatttttg ccagacggtg cccgaatgcc ggcctccaca
     6541 atcttccgca aatgttcgcg cgggacgggc acatcccgaa atggtccccg atagctgtac
     6601 cgcttgttga tcgcttcaaa aacatccatg gcgtctcctc ccgaaatcaa tgcatcgtgt
     6661 gggttattgg cataccagct cggcacgagg tgctttcttg tcaacgaccg ggaatgtctc
     6721 tatgttaccc ttacactgct gtggggttaa gtctttcgcg cggcaaaagg aaagtgcgaa
     6781 gtgcggaaac ggt
//
LOCUS       DAOSUT010000197         6749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5454_length_6749_cov_8.111144, whole genome shotgun sequence.
ACCESSION   DAOSUT010000197 DAOSUT010000000
VERSION     DAOSUT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5454_length_6749_cov_8.111144"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..757)
                     /locus_tag="PLD05_13965"
     CDS             complement(194..757)
                     /locus_tag="PLD05_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78595.1"
                     /translation="MKRFVAILVLIPSALLIAGTVTGCKVVMSTNPIGVEAVKLNPEE
                     WNGIWRDPVNNGFVAVRVADADQGILEAAGIETRDDGLELKKLRLHVRQANSWMFLSL
                     ESPDRPGNYFWWRIKRSGEAAILWAPHWDRISQLVREGKLPGKQVSENVLILDELGPK
                     HYEVLTSEGSIVVDWENPIMLIRAKKD"
     gene            1027..>3398
                     /locus_tag="PLD05_13970"
     CDS             1027..>3398
                     /locus_tag="PLD05_13970"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOP78596.1"
                     /translation="MVFAFCCRMIRFFIAAMCLAEIQCLSMGASAVDVGKSLVPVRSA
                     RVRTIEGIPRLVLNGQPVPARIFWGAPGRSAIQAGPTGQSVTFEFVAYDDAERQGTLH
                     FRFGRLPGSVLLDSIRVQDVDTGDTILSCDFEDEQDFSANWSVWPPDERNTVGHVERL
                     SNSGDNGSGCLAIHLNKPPQGEWPDFHIYSRPSLPIVKGRRYRVKLWIQSDVPRKVMI
                     AFYRPGSPFVLLGGPPGPFRSQVRLAAQSGVNLVSFPVPMPWPKPGEPEDWTAVDFAC
                     REVIEANPNALLIPRIPMDPPAWWIQSHPDHAMTWDRPGQTRVPASVASPLYRDEAAH
                     RLTRLVSYLETRWGDHMAGYHPCGQNTGEWFYENTWGAALSDYSPVTAQAWRQWLVKK
                     YGSDEALQKAWENPNVQLATIDLPTPDRRRSNPSGILHRFKTEQDLIDLAEFQQDMMA
                     ECVCHFAKAVREASRGQKLVVFFYGYTFEFGAIHNGASTSGHYALAKVLRSPDIDILC
                     SPISYWDRGIGGSAPAMSAAESVMLAGKLWLFEDDTRTYLAKESRAPGWMDGADTLHE
                     TQWLLMRNTAQVALRHFGTWWMDLGATGWFDDPELWSVMRQLQPLTEFMLSRGRPFEP
                     EVAAIVDEKSILHVGYGGNVLTRPLIYEIRRPLGRMGTPYGQYLLEDVINQRVPAKML
                     VFLAAWRLSKDERQELRKTTAGKLKIWCYAPGYLAPGEEALAAMKELTSYDLKEISGV
                     VAWAEPTEEGRRLGLTQGFGIRQPIRPLLAARDVRPEEVLAAYPDGSAAVV"
     assembly_gap    3399..3498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3562..5796
                     /locus_tag="PLD05_13975"
     CDS             3562..5796
                     /locus_tag="PLD05_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78597.1"
                     /translation="MSKSTSLMVIIACGLLAGLICVASENPQKAFNSSQLSGPGNFFV
                     APNGNDKWSGRLPVPNQDQSDGPWATLEHARDVLRKLRAEPDSRERLPKTITVWVRGG
                     VYWLSRPLIFEAEDSGKSGSPVTYRAYPGEKPILRGGRPISNWTVHQGQILKADLSRT
                     PFHGIYFRQLFFDGKRQHLARYPNFDPTKPYTGGWAYVDGKPVPMYSEIPGENRRTLR
                     IKREDLRNWSRPEEAEVCVFPRYNWWNNILRIASLDRQNGIATLAANASYPIRPNDRY
                     YLRNLFEELDAPGEWYLDKNTWTVYFWPPGDIRAAEVYAPIIETLIEIRDASDIIIQG
                     FTLECCEGTAVLLRNTRDCLIAANTIFNVGGRCQPADAAVRVEDGSGNGVVGNDIYEV
                     GSHAIVLTGGDRTTLRAAGNYADNNYIHHTGVFYKQGVGVVLQGVGNRASHNLIHDCP
                     RFGIQYSGNDHVIEFNHIRHVNLETADTGVIYSGGRDWVSPRGTVVRYNYFHDSIGFG
                     QQNGQWTSPHYAWGIYLDDNSAEVHVYGNIVVRAIRGLIHFHCARDNLVENNIFVDGT
                     MQQLEMNGWPDHSRHIETMTLGYEQYAKLPAWQKYVGLQKGGHPKDAIPIANNRFVRN
                     IIYYHEPNAFAYKLRRLRLEHFECDNNILWHQGGSVLISLDDKPRTFQEWQQLGFDLH
                     SLVADPKFVDPAKDDYRLQPDSPAFQLSFQPIPVEKIGPYADSLRASWPIQEAPGVRE
                     MSYK"
     gene            6083..6169
                     /locus_tag="PLD05_13980"
     tRNA            6083..6169
                     /locus_tag="PLD05_13980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6117..6119,aa:Ser,seq:tga)
     gene            6531..>6749
                     /locus_tag="PLD05_13985"
     CDS             6531..>6749
                     /locus_tag="PLD05_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78598.1"
                     /translation="MRLTRSEIEEGLFLRVQGNWGRDRVLWGQVLRWPHVTCKTASLI
                     TFLREVLTSTFASLGCLRGLTELVEYELS"
BASE COUNT         1516 a         1767 c         1783 g         1583 t
ORIGIN      
        1 cgggcgttgg ctcgaaattc cggttgaagc gtccatgacc tcggcaggct ctctcttggt
       61 tcgcatcgtc aaccgtaatc ctcgctcgaa tgccgtggta tccattcttg aatgggtcga
      121 agccacggca tcaaaccagt gattccgtgg cttctggaat cgcgattagc gccccaggcc
      181 aatcgttgcc agcctaatct tttttcgccc taataagcat gatgggattt tcccaatcca
      241 ccacaatgct gccttctgaa gtgagtactt catagtgctt tggaccaagc tcatccaaga
      301 taagcacatt ctcactgacc tgttttcctg gcagcttgcc ttccctgacg agttgagaaa
      361 ttcgatccca atgaggagcc cataaaatgg ccgcctctcc tgagcgttta atccgccacc
      421 agaagtagtt tcccggacga tcgggagatt ccaaggaaag aaacatccag ctgttagctt
      481 ggcggacatg gagacgaagc ttcttcaatt ccaagccgtc atcgcgtgtc tcgatgcctg
      541 ccgcctcgag aatcccttga tctgcgtccg ccacacgcac agcaacgaac ccattgttga
      601 ccggatcgcg ccaaatgcca ttccattcct cgggattaag cttgacggcc tccactccga
      661 tgggattagt tgacatcacc accttgcaac cggtcacagt acccgcaatc aacagagcac
      721 tcgggatgag gactaagata gctacgaatc ttttcatcgt tgtgtctcct ctgctggagt
      781 atgaagcaca cgcccctgca taccaaccta attatacggt atgaaacctc gcgctcacca
      841 aggatcgagg gcggctccgt gatggtctct ggacagacac gcgcggctgg cccataatcg
      901 tggcaacgcc tgtggttttt acgggcagca gaccaagatc tcagcgagat ttgtcaattg
      961 ttctatgcaa atgaaagagg agcatctccc gtgaagggcc cacacgatcc accttcgatg
     1021 gacaggatgg tattcgcatt ctgttgtcga atgattcgct tttttattgc cgccatgtgc
     1081 ctcgcggaaa tccaatgctt gagtatggga gcctccgccg tcgatgtcgg caagtcactg
     1141 gttccagtcc ggagtgcacg tgttcgtacc attgaaggca ttccgaggct tgtattgaat
     1201 ggtcagcctg ttccggcccg aatcttttgg ggtgcgccgg gccggagcgc tatccaggcg
     1261 ggaccaacag gtcaatcggt gacttttgag ttcgttgcct acgatgacgc ggaacgtcag
     1321 gggacgcttc acttccgctt tggtaggctg cccggttccg tcctgctcga cagtattcga
     1381 gtccaggatg ttgacacggg cgacacgatc ctttcctgcg attttgagga cgagcaagat
     1441 ttttcggcaa attggtccgt ctggcccccg gacgaacgaa acacagtggg gcatgtggaa
     1501 agactttcga actctggaga taacggcagt ggctgcttgg ccattcatct caataagccc
     1561 ccacagggtg aatggccgga ttttcacatc tactcgcgtc cttctctgcc gattgtgaaa
     1621 ggacgacgtt accgcgtaaa gctctggata caatcggatg ttccccgaaa agtgatgatc
     1681 gcgttttacc gtccgggttc gcctttcgta ctgctgggcg gccctcctgg accgtttcgg
     1741 tcacaggtac gtcttgctgc tcaatctggc gtgaacctgg tcagttttcc cgtgccaatg
     1801 ccgtggccaa aaccaggcga accagaggac tggacagccg tggattttgc ctgccgagaa
     1861 gtgatcgaag ccaaccccaa tgcactgctc attccccgga ttccgatgga cccacccgcc
     1921 tggtggattc aatcgcatcc agaccacgcc atgacttggg atcggcccgg tcaaacgcgc
     1981 gttcctgcgt ccgtcgcatc acctctctat cgagatgaag cggcccaccg gctcacccga
     2041 ctcgtgtcgt atctggaaac acgctggggc gatcacatgg cgggctatca cccctgtggt
     2101 caaaacaccg gtgaatggtt ctacgaaaac acttggggag ccgctctcag tgattattca
     2161 cctgtcacag cgcaggcgtg gagacaatgg ttggtcaaaa aatatggcag tgatgaagcc
     2221 ttacaaaagg cttgggaaaa tcccaatgtg caattggcga caattgatct cccgacaccc
     2281 gatcgcagac gatccaatcc ttcgggcatt ttacatcgtt ttaagacgga acaagacctg
     2341 attgacttgg ccgagttcca gcaggacatg atggccgagt gtgtttgtca ttttgccaaa
     2401 gctgtcaggg aggcatcccg cggacaaaaa ttggtcgttt tcttttacgg atacacattt
     2461 gaattcggag cgatccataa cggagcatcg acgtcgggcc actatgcact cgctaaagtc
     2521 cttaggtcgc cggacatcga catcttatgt tcgcccattt catactggga tcgcggaatt
     2581 ggaggaagcg cgccggcgat gtccgcagca gaaagcgtca tgctggcagg caagttgtgg
     2641 ctatttgagg atgacacgcg gacatatttg gccaaggaaa gtcgagcacc aggatggatg
     2701 gacggcgcgg atacgcttca cgaaactcag tggttgttaa tgcgtaacac ggcccaggtt
     2761 gccctgagac attttggaac gtggtggatg gacctcggtg cgacgggctg gttcgacgac
     2821 cctgagctgt ggagcgttat gagacagctt cagccgctga ccgagtttat gctttcgcga
     2881 gggcgcccct ttgaaccaga ggttgccgcc attgtcgatg aaaaaagcat cctccacgtc
     2941 gggtatggcg gtaatgtgct tacgcggccg ctcatttacg agattcgtcg gcccctaggc
     3001 cgaatgggaa cgccttacgg gcagtatctt ttggaagacg ttatcaacca acgggtcccc
     3061 gctaaaatgc tcgtgttttt ggccgcgtgg cgactttcca aagacgagcg tcaggaactg
     3121 cgcaaaacga ccgcgggcaa actcaaaatc tggtgttatg cgccaggata tctggcacct
     3181 ggggaggaag ccctggccgc aatgaaggaa ctcacgagct atgatctgaa ggaaatttct
     3241 ggagtcgtgg cttgggccga gccaacagag gaaggacgcc gactaggttt aacgcaaggg
     3301 ttcggcatcc gtcagcccat tcggccgttg ctggcggcac gggacgttcg acccgaagaa
     3361 gtcttggcag cctaccccga cggaagtgct gcggttgtnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnncg ggaacgacca attgctgcgt cagccgtaca aagattcctt
     3541 tttcataggg aggtctcaat tatgtcgaaa tccacttctt tgatggttat tattgcgtgc
     3601 ggcctacttg caggtctgat ctgcgtggcc agcgaaaacc cgcaaaaggc gttcaatagt
     3661 tctcagctat ccggaccggg aaacttcttt gtcgcaccga atggcaacga caagtggtcc
     3721 gggcgtttgc ctgtccccaa tcaagatcaa agcgatggcc cctgggcaac tctcgagcat
     3781 gcgagagacg ttttgaggaa gcttcgcgcc gagccagatt cccgtgagcg ccttcccaag
     3841 acgattacgg tgtgggtccg agggggtgtc tattggcttt cccgaccact tatcttcgag
     3901 gcggaagact cgggaaagag cggaagcccc gttacctacc gcgcgtatcc cggagaaaag
     3961 ccaatccttc gtggcggtcg ccccatttca aactggacag ttcatcaggg acaaattctc
     4021 aaagctgatt tatcgcggac tccgttccac gggatttatt ttcgccaact ctttttcgac
     4081 ggaaaacgtc agcatctcgc ccgatatccc aatttcgatc caacaaaacc ttacacgggt
     4141 ggatgggcct atgtcgatgg taaacctgtc cctatgtact cggaaatccc tggtgaaaac
     4201 cgacggaccc tccgtatcaa acgcgaagac ctccgtaact ggagtcgtcc cgaagaagcg
     4261 gaggtctgcg tcttcccacg atataactgg tggaacaaca ttcttcgcat tgcgtctctt
     4321 gatcggcaaa acggaatagc gacattagcc gccaatgcct cttacccgat tcgcccaaat
     4381 gaccgatact acttgcgaaa cctattcgaa gaactagacg caccgggtga atggtacctc
     4441 gataaaaaca cgtggaccgt ctatttctgg ccacccggcg atatccgggc agcagaagtt
     4501 tacgcgccta ttatcgagac actcattgaa attagagacg cgtctgacat tattattcag
     4561 ggattcactt tggagtgttg tgagggcaca gccgtactcc ttcgtaacac ccgagattgc
     4621 ttgattgcgg ctaacaccat attcaacgtt ggtggccgat gtcaaccagc cgatgccgcc
     4681 gttcgtgttg aagacgggtc gggcaacggc gtcgttggca atgatatcta tgaggtcgga
     4741 agtcacgcga ttgtgctcac cgggggcgat cgcacaacac ttcgggcggc cggaaactat
     4801 gccgacaaca actacattca ccatacaggt gtgttttaca agcagggagt cggtgtggtt
     4861 cttcaaggtg tgggaaatcg ggcctcgcat aatttgatcc atgattgtcc ccgatttgga
     4921 attcagtact ccggcaacga ccatgtgatt gagttcaatc atattaggca cgttaactta
     4981 gaaaccgcgg acacgggagt tatctattcg ggtggtcgag attgggtttc accacgtggc
     5041 accgttgtcc gctataacta tttccacgac agtatcggtt ttggacaaca aaatggccaa
     5101 tggacctcgc cacactatgc ttggggcatt tacctagacg acaactcagc ggaggttcac
     5161 gtgtatggaa atattgtggt ccgagcaatt agaggattga ttcatttcca ttgtgctcga
     5221 gataaccttg tggaaaacaa tatctttgtt gatggaacca tgcaacaact ggagatgaat
     5281 ggctggcccg atcattcccg ccatatcgaa accatgacgt taggctacga acagtatgca
     5341 aagctgccgg cctggcaaaa gtacgtcggg ctacaaaaag ggggacatcc taaggatgcc
     5401 atccccatcg caaacaaccg ttttgttcgg aacatcattt actaccacga gccgaatgcc
     5461 tttgcgtaca aattgcggcg actacgactg gagcattttg aatgcgacaa taatatcctg
     5521 tggcatcaag gcgggtcagt gttgatcagt ctagatgata agcctcgaac ctttcaggaa
     5581 tggcaacaac ttggtttcga ccttcactcg ctggttgccg atcctaagtt tgtcgatccc
     5641 gccaaagatg attatcgact ccagcccgac tcaccagcct ttcaactgag ctttcagcct
     5701 atcccggtcg aaaagatcgg tccctatgcc gactccctcc gggcaagctg gccaatacag
     5761 gaggcccccg gcgtgaggga aatgtcatac aaatgagctg ccccaaaata agttggtcgt
     5821 ccttccggct gctctgctga ttgtgtcgag taaccagact tttcataaag tgatccagcc
     5881 ggcacgtccc cttgggacct ttacggagtc ggttcagact ggtaaagtag gttacgttag
     5941 ccgggttcga tctcattgaa taagacgaat accgcgcagc cgagaactac cgcatcgcac
     6001 acgcaggcgg ggtttagctt gagtgtgtcg cgttcgatgt gggtgttgtt ttattacacc
     6061 cacccgagtg gaattgctag gtggatgggt ggccgagtgg tctaaggcgg cagtcttgaa
     6121 aactgctgta cccgttcggg taccgggggt tcgaatccct ccccatccga tggatgtctg
     6181 gccggggcct tgcagactgt cgtaagccac gaaatggcgg tgactaaccg ttcggagtgt
     6241 actacccttt tccgttcttt acatcgtttc ttggctgaag cccaactgtt tttctcgatg
     6301 gggccatgtt cagtctggtg ttctccgtgt gcctcgatgc ctgaacgccg cgctttggca
     6361 acttgcgagg tggagttgtc ggcaacattc actacatctt ggacgatacc taaaccccac
     6421 atgaagccag ctgcctgcat gatttccttt gccgacgatt ccacaaggga ttaacggtgg
     6481 aaggacaatc gcggtcaacg gatcatcccc gcgtgggcgc ggaaactctt atgcgactaa
     6541 cgcggtcgga aatagaggag ggattatttc tgcgcgtgca gggaaactgg gggcgagata
     6601 gagttctgtg ggggcaggtc cttcgctggc cgcacgtcac ttgcaagact gcgagcctca
     6661 ttaccttttt gcgcgaggtt ttgacctcga cttttgccag tcttgggtgc ctacgtggct
     6721 tgacagagtt ggtcgaatat gagttgtct
//
LOCUS       DAOSUT010000198         6657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5531_length_6657_cov_9.407907, whole genome shotgun sequence.
ACCESSION   DAOSUT010000198 DAOSUT010000000
VERSION     DAOSUT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5531_length_6657_cov_9.407907"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="amrB"
                     /locus_tag="PLD05_13990"
     CDS             <1..614
                     /gene="amrB"
                     /locus_tag="PLD05_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOP78599.1"
                     /translation="VIIVAPKHQALGCDWAVAPHETWALPGLSLKSNAQLARACAENV
                     PYFELDAAAHAIEHAIEVQLPIIARLAPASRVVGVVMRDGPYPALRGAAKKFAEFLQA
                     LPELPLLVISTDMSHYETESKARELDRLAIEAIKSCDPERLWKTCHEHNISMCGIVPA
                     VFVMETLKELGRLETCHVVGYTTSAESSGDSSRVVGYLGALFA"
     gene            1221..2618
                     /locus_tag="PLD05_13995"
     CDS             1221..2618
                     /locus_tag="PLD05_13995"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP78600.1"
                     /translation="MADVVANYAPPWLISGIVHVLILIVMALIMLPQFFRPEVELTAE
                     IYAEKLGDQLEFDSPFAGTDREKLEEPLWTPPDLPKVTDPFAAPPPSELVPEGVTSTS
                     DIQAQVIGYALKGRDAGSRQALLGLYGGTKTTEAAVELGLQWLSRQQRKDGSWSLTGP
                     YPNGCETENPEAATALALIAFQGHGNTHKTGPFRDNVSRAWNWLLQQQDANGNFFHEG
                     NFNHPFYTQGLCTIAVCEIYGMTKDERFREPALRAIDYLIKSQSSEGGWRYTANTDSD
                     VSVTGWIVMALQSARMAGFEVPQDVFRKVERFLDRVAIEGGSKYPYQKGGQASLAMTA
                     EALLMRQFLGWSHNDDRLAKGLAWITEPANLVNFERDRDVYYWYYATQVCHHMEGEYW
                     KKWNEVMRQLIPQHQVKSGPQAGSWDPTKPSRDAWANEGGRLYVTCLSIYCLEVYYRH
                     LPLYSQRALRLVGGM"
     gene            2735..>4155
                     /gene="zwf"
                     /locus_tag="PLD05_14000"
     CDS             2735..>4155
                     /gene="zwf"
                     /locus_tag="PLD05_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOP78601.1"
                     /translation="MAHTIVIFGASGDLTRRKLIPALYELHRKKRLPPETTIVGMSRT
                     QYSHDQWREVLREATAAQVGPKLLPDLWDEFARRIYYYPGDVSRAEDFPGLNAFISSL
                     EAEKNTTRVYYLSLAPEFYECTVEHLGQAGMAHETHAPRRIVVEKPFGRDLQSARALN
                     AKIHEVFAEHQIYRIDHYLGKETVANVLVLRFANTIFEPIWNRRYVDHVQITAAENLP
                     VGRRAAFYESAGILRDMFQNHLLQLLTLTAMEPPSRVNADDIRDEKVKVLRAVRPFTR
                     ADVAANTVRGQYRKYRDEPGVPPNSQTATFGAVKLYVDNWRWQGVPFYLRSGKAMSCR
                     TTQIVIQFREPPHMMFEGGPRRIHEANRLVLQIQPAEGIQIHFQTKVPDAGMKMRLTD
                     LDFNFREKFAGEMPEAYERLLLDVMMGDPSLFARADEVELAWNIVDPIQEAWDEGGPP
                     QLELYEAGGWGPPYSEEWMQRQGR"
     assembly_gap    4156..4255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4256..5041
                     /locus_tag="PLD05_14005"
     CDS             <4256..5041
                     /locus_tag="PLD05_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78602.1"
                     /translation="WLQSRGERPFFLYVPFTAVHIPIREPEAYLKRVPPTIEEPSLRE
                     YAACIIHLDEAVGKLLEVLEKSGKASQTLVVFTSDNGGTTARNDDPAYPPDGYAPGRS
                     GGNNRPLRGNKGTVYEGGIRVPTVARWPGVLKPGKYVHPVHITDWMPTFCALAGYCPE
                     KPLNWDGQNIWPFLTGAAEPASRTIYTVGPSFRSVAIRDGDWKLIIHRATGPESERVE
                     LYNLASDPYEAEDQAENRPEQVAMLKAKLEGVAANDRDAVAEK"
     gene            5284..6597
                     /locus_tag="PLD05_14010"
     CDS             5284..6597
                     /locus_tag="PLD05_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOP78603.1"
                     /translation="MDEQVCEIRNNFGDVGRVLRPQCWGLFGGCGAFAQEIVGMEAIA
                     HSCSQFDQLVYARDILQCAGQAILRVSRRLGDSFCRAVELLLACRGHVVVSGVGKAGL
                     IGQKISATLASTGTPSHFLHPTEALHGDLGRVRLGDVGLLLSASGETEEMVRLSTALT
                     ELQIPIIAMTCSRQSQLARSAQVVLELGPIEEADHLRLAPSTSTTVMLALGDALALTV
                     SRLRGFRAEDFARFHPGGSLGKKLSRVEEHMRPLSECRLASDQLTLRQVIVATRRPGR
                     RTGAIMLLDAEGKLSGIFTDSDLAKVLERRQDSVLDAPIADLMTRHPITVRAGTSLRD
                     AVRVLAEKKISELPVVNENGNPVGLLDITDVIALFPDLLSQNGQGTFDERTSTESQQP
                     GDSSEDETPSRPPEPRSNMFRSSQPPGLPELRIVVPEECEYPNER"
BASE COUNT         1496 a         1824 c         1838 g         1399 t
ORIGIN      
        1 acgtgatcat tgtggctcct aaacatcagg ccctcgggtg cgattgggca gtggcaccac
       61 acgaaacatg ggcactcccg gggctgagtt tgaaatctaa cgcccagctt gcccgagcct
      121 gtgcggaaaa tgtgccgtac tttgagctgg acgctgcagc gcacgcgata gaacacgcaa
      181 ttgaagttca actcccgatc attgcccgcc ttgcgccagc aagccgggtg gttggagtcg
      241 tgatgcggga cggcccgtac ccagcgcttc gcggggcggc aaagaagttt gcagagtttc
      301 tccaagcgct gccggaactt cctctattgg tgatctcgac cgatatgagc cattacgaaa
      361 ccgaaagcaa ggcgcgagag ttggatcgac tggctatcga ggccatcaaa tcctgtgatc
      421 ccgagaggtt gtggaagact tgccacgagc acaacatcag tatgtgcggc attgtcccgg
      481 ccgtttttgt catggaaacg ctcaaagagc tcggacggct agaaacatgc catgtggtcg
      541 ggtacaccac cagtgccgaa agctccgggg actcgtcgcg ggtagtcggg tacctggggg
      601 ccctttttgc ctaaaaacgg aaacaaccaa cactttttca ccgaacatga ctcgaaataa
      661 gatcaccgac acagcaacgc attctcccag cctctcagcg gtaacaatct ccaaaagggt
      721 cttggtgttt gaactgtgaa ttgttgcgga gaagcgaaaa aagtacacta aggcaaggtg
      781 tctttttcag gtatcggaac acagtagagc agcctttttc gtagaatcgc cctgtcatcc
      841 cgcttaacgc attgacgagc catatggaat ttgggccatc ccattcacaa aacgacaacc
      901 caacaccggg aaaagagttt gagaggcccg tgccccagac ggttcgtcct ctaccgctcg
      961 gcagccgcca agagagcctg gcgggccaac gcgagtcgcc gccgacgaat atccccaggg
     1021 cgacgccgac tcaagatccc gccactcaag cgacgagtgg tgtccagagg tccccactgg
     1081 tccccatcgg ggcccccacg gagcaaggac gaggttcgtc cactggtaga atcgcaccgg
     1141 tcagagctgg cgcaggggtg aaatcgctag ggcgaggtcc tgcaggggca atatcggccc
     1201 cggcgccgga gacggaagaa ttggcagacg tcgttgccaa ttatgccccg ccctggctga
     1261 taagcggtat tgttcacgtg ctcatcttga tcgtcatggc cctgatcatg ttacctcagt
     1321 tctttcgccc tgaggtcgaa ttgacggccg aaatttatgc ggaaaaattg ggagaccaac
     1381 tggagttcga ttccccgttt gcgggaaccg atcgcgagaa gttggaagag ccgctatgga
     1441 cgcctcctga tcttcccaag gtaaccgatc cttttgccgc accgccgcct tccgagctag
     1501 tccccgaagg tgtgacttcg accagtgaca ttcaagccca ggtcattgga tacgcgctca
     1561 aggggcggga cgccggaagc agacaagccc tgctcgggct ttacggcggc acaaaaacga
     1621 cggaggccgc cgtagaattg gggctccaat ggcttagccg gcaacagcgt aaagacggtt
     1681 cgtggagtct taccggcccg tatcccaacg ggtgcgaaac ggaaaaccca gaggctgcca
     1741 ccgcccttgc gctaatcgcc tttcaagggc acggaaacac acacaaaacg gggccattta
     1801 gggataatgt ctcccgcgcg tggaattggc tgcttcagca acaagacgcc aatggcaatt
     1861 tctttcatga aggtaatttc aatcatccct tttacacgca agggctttgt actatcgccg
     1921 tgtgcgaaat ttatggcatg acaaaggatg agcgattccg tgagccagca ttacgggcaa
     1981 ttgattacct catcaaatca caaagttctg agggtggctg gcgatacacc gccaatacgg
     2041 atagcgatgt ctctgtcacc ggttggattg taatggccct tcaaagtgcc cgcatggccg
     2101 gtttcgaggt gccgcaggat gtcttccgaa aagtggaacg cttcttggac cgtgtcgcca
     2161 tcgagggagg cagcaagtat ccctatcaaa agggcggtca agctagcctc gccatgactg
     2221 ctgaggcgct cttgatgcgg caattcttgg gctggtcgca taacgacgac cgccttgcga
     2281 aaggcttggc ttggatcaca gaaccggcga atctcgtgaa ttttgaacgc gatcgggacg
     2341 tctattactg gtattacgcc acgcaggtgt gccaccacat ggagggcgaa tactggaaaa
     2401 aatggaacga ggtgatgcga cagttgattc cccaacacca ggtcaaatcg ggcccccaag
     2461 ccgggagttg ggaccctaca aagccttcac gcgatgcttg ggcaaacgag gggggacgac
     2521 tctacgtgac ctgcctgtcc atctactgtt tggaggtcta ttaccgtcat ttgcccttgt
     2581 attctcaacg ggccttgcga ctggtgggtg gtatgtgata ccgggtcccc ttggctgttg
     2641 accgacctgg ataataatag caacgtcaac aaagcggtta tcggcccatt tggcaatcag
     2701 tcttcgcagg ggtcaaggcg tttgtggaat agccatggca cacacaattg tcatttttgg
     2761 agcatcgggt gatctaacgc gacgaaagct tatcccggcc ctctacgagc tgcatcgaaa
     2821 aaaacggtta cccccggaaa caaccatcgt cgggatgtcc agaactcagt attcgcacga
     2881 ccaatggcgt gaggtcctca gagaagcaac ggcggcccag gtcggcccca agcttcttcc
     2941 agatctgtgg gatgaattcg cacgtcgcat ctactactat ccgggagacg tcagccgggc
     3001 agaagatttt cccggcttga atgccttcat ttcgagtctc gaggcagaaa aaaataccac
     3061 acgggtttat tatctctcgc ttgctccaga gttctatgaa tgcacggtgg agcatcttgg
     3121 ccaggcggga atggctcatg aaacgcatgc ccctcggcga attgtcgtgg aaaaaccgtt
     3181 cggccgtgat cttcaatccg cacgcgccct gaacgccaag atccacgagg tcttcgccga
     3241 gcatcagata tatcggatcg atcactactt gggcaaagag acggtggcca acgtcctcgt
     3301 cttgcgcttt gccaatacca tctttgaacc tatttggaac cggcggtatg tcgatcatgt
     3361 gcagatcacg gcggcagaga acctgccggt cggacggcgg gccgcgtttt acgagtcggc
     3421 aggcattctt cgcgacatgt ttcagaacca tctattgcaa ctcttgacat tgacggccat
     3481 ggaaccgccg agccgggtta acgccgatga tatccgagat gaaaaggtta aggttcttcg
     3541 ggccgtgcgg cctttcaccc gagccgacgt cgctgcgaat accgtccgcg ggcagtaccg
     3601 gaaataccgc gatgaaccgg gagttccccc aaatagtcaa acggcgacgt ttggtgcagt
     3661 aaaactgtac gtggacaatt ggcgatggca gggagttcct ttctatttgc gctccggcaa
     3721 ggccatgtcg tgccgcacaa cgcagatcgt gatccaattt cgggagccac ctcacatgat
     3781 gttcgaaggt ggtccccgcc gcatccacga ggcgaaccgg ttggtcctac agattcaacc
     3841 tgcagaaggc atccaaatcc attttcaaac gaaggttccg gacgcgggca tgaaaatgcg
     3901 tctaacggac ctagacttta acttccgtga aaaattcgcc ggggaaatgc cagaggcgta
     3961 cgaacgcctg cttctcgatg tcatgatggg cgatcctagt ctttttgccc gcgccgacga
     4021 agttgagctc gcctggaaca ttgtagatcc catccaagag gcctgggacg aaggggggcc
     4081 tccacaactg gagctctatg aggccggggg atggggtccg ccctattccg aagaatggat
     4141 gcagcgacaa ggccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggct
     4261 tcaatcccgc ggcgaacggc ccttcttcct ctatgtgccg tttacggcgg tacacattcc
     4321 cattcgtgaa ccagaagcat atctcaaacg ggttcccccc acgattgagg aaccatctct
     4381 tcgggaatat gccgcctgca tcatccatct tgacgaggcc gtaggcaagc tgctcgaggt
     4441 gctggaaaag tcggggaaag cttctcagac tctcgtcgtt tttacaagcg ataatggcgg
     4501 gaccacggct cgcaatgatg atcctgccta cccgcccgat ggctacgcgc cggggcgatc
     4561 cggcggcaat aaccgccctc tgagaggaaa caaagggacc gtgtatgagg ggggcattcg
     4621 cgtccccacg gtggcccgat ggccaggtgt cctgaagccg ggcaagtacg ttcatccggt
     4681 tcacattacc gattggatgc ccactttctg cgcactcgcg ggatattgcc ccgaaaaacc
     4741 gcttaattgg gatgggcaaa atatctggcc gtttttaacg ggcgccgccg agccggcttc
     4801 gcgaaccatc tacacggtcg gtccaagttt tcgttcggtt gccattcgtg atggagattg
     4861 gaaactcatc attcaccggg ccaccggtcc cgaatcagag cgggtggaac tgtacaacct
     4921 agcgagcgac ccctacgaag cggaggacca agccgagaac aggccggaac aggtcgcaat
     4981 gttgaaagcc aaacttgagg gcgtagcggc aaacgaccgg gatgcagtgg ccgaaaagtg
     5041 acagcattta tccgtcctta gtggcagtga acatcacgcg gaggagattt tcggccgatt
     5101 gcccttttgg aggctcgtcg cgattctcgt acaatcccca tattgccaag tttcgtgtct
     5161 tgcccacatg ttacatgcca cccatctttt ccacatatac cggcatagtc gggttccaag
     5221 atgcagtcac gttgctgaag ttttccgccc aagctacaat ttcctccgac gaattggcga
     5281 atcgtggacg aacaagtctg cgaaatccgt aataatttcg gggacgtagg tcgggttttg
     5341 agaccccaat gttgggggct ctttggcggg tgtggagctt ttgcccagga gatcgttggc
     5401 atggaggcca ttgcccattc ttgctcccaa tttgaccaac ttgtctatgc ccgggatatt
     5461 ctccaatgcg cgggacaagc aattctgagg gtatcacgcc gtctgggcga ctcgttctgt
     5521 agggccgtcg agctgttact ggcttgtcgg ggccatgttg tagtctcagg agtgggtaaa
     5581 gccggcctga tcgggcaaaa aatctcggca acgcttgcat cgacgggaac gcccagccat
     5641 tttcttcatc ccacagaggc ccttcacggg gatctcggtc gcgtccggct cggcgatgtt
     5701 gggctccttc tctcggcaag tggcgagacg gaggagatgg tcaggctctc gaccgccctt
     5761 accgagctgc aaatcccaat catcgccatg acatgctccc ggcagagtca acttgccaga
     5821 tcggcacaag tagttctcga actgggcccc atcgaagaag ccgaccatct gcgactggcc
     5881 cccagcacga gcacaaccgt tatgttggcc ctgggtgacg ccctcgccct tactgtaagc
     5941 cgtcttcgag gatttcgggc agaggacttc gcccggttcc atcctggggg aagccttggc
     6001 aaaaagctaa gccgagtcga ggagcatatg cgtcccctaa gtgaatgccg cttagcttct
     6061 gaccaattaa cactgcggca agtcatcgtc gcgacacgtc gtcccggtag aaggacgggg
     6121 gcaatcatgc ttcttgacgc tgaaggcaaa ctatcgggga tttttacgga cagtgacctt
     6181 gccaaggtct tggaacgtcg gcaggactcc gttctcgacg caccgatcgc cgatctgatg
     6241 acacgccacc cgattaccgt tcgtgctggg acttcgcttc gggatgccgt cagggtcttg
     6301 gccgaaaaga agatcagtga acttcccgtc gtgaacgaaa atggaaatcc agttggcttg
     6361 ttggacatca cggacgtcat tgccctcttt ccggatctgc tttcccaaaa tgggcaaggc
     6421 acatttgatg aacgcacatc gactgagtct caacagcccg gggattcttc cgaggatgaa
     6481 acgccgtccc ggccccctga accgcgatcc aacatgtttc gctcgagtca gccgccggga
     6541 ttacctgagt tacggatcgt ggtcccggag gaatgcgaat accctaatga gagatagcgc
     6601 ctttttcgcc ggcttacgag actggcacat gacacatgac attctccgca atttggg
//
LOCUS       DAOSUT010000199         6653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5533_length_6653_cov_7.979236, whole genome shotgun sequence.
ACCESSION   DAOSUT010000199 DAOSUT010000000
VERSION     DAOSUT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5533_length_6653_cov_7.979236"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..4817)
                     /locus_tag="PLD05_14015"
     CDS             complement(399..4817)
                     /locus_tag="PLD05_14015"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOP78604.1"
                     /translation="MHTYRGRQGADVAMLIRRLKERCAARDLVHIGTSATMVASRQAT
                     PAERRAAVADFAGRLFGHSFQAEDVIEETLVPFTQGGPPTVEELRKAMTDGDWQKDAE
                     SFRRHPLVRWVEYALGIEKDAEGNLRRRVPRTLSGAADELAELTGIARHRCQEYLRQV
                     LELGGAMPGEGGNRALAFKLHQFISQGRALFATLEPAPQREFSLEGQTQAGGGRIFVP
                     VKFCRQCGQDYYHVLQTNPPSPSGRGVGGEGTSPSPFGRGVGGEGCFLPHPIGIELGQ
                     DEEEQQAGYLMLAPAEEDWSEDRIPEEWYDASGRFKNSWRDRVPTAVWVAPDGTYWRN
                     SRAGAIKMWWQKEPFSLCLRCGEFYTAKERDFAKLASLSSEGRSSATTVLAVSLLRHA
                     SGGDGPRDKLLSFTDNRQDASLQAGHFNDFVHVSLIRCALYAALKQKSELTFSEVAKH
                     VVECSGLTIRDIARNPELAPESAAARDVWKVFTELTEYRLYEDLRRGWRVVQPNLEHV
                     GLLRIGYRGLEDLCAGEKYWQFHPNVARLSPAERETIVRAVLDQFRRKLAIRCDCLRE
                     TEQQQIRKRAEQQLNEFWGLDPDANELCPAARYVRLGQSRQLALGYRLGERSAIGRFL
                     RQRLGLSTDEYLTFLDALLKLLVEQGFLVREVKDDHQFYQLDAACLVWRLGDGTPPPD
                     PMYSRRVPPVNAFFQRFYQEWPGFLAGLEAREHTAQVVRPGERERRERRFRWEESDTQ
                     KEREVGRRLPYLVCSPTMELGVDIADLDFVHMRNVPPTPANYAQRSGRAGRQGQPGLI
                     FTYCGALNSHDQYFFNRRDEMVAGSVRPPRLDLTNEALLRAHVHAMWLAQVRLPLGQS
                     IEDVIDREQDDLPLKTEVAGAIQLGEAARYELRRRVQNVLAPDMGILEQTGWFNEDWI
                     DRVLDEAPQQFDRAFDRWRELYRAAVQQREAAYNELKRARTIEDQDKADRKMKEAQRQ
                     LNLLCQIDVAREEGDFYPYRYLASEGFLPGYNFPALPVRAWVPRDEGEFIGRPRFLAI
                     RELAPNNILYHEGTKWEVVSLQSPPGGLDERLVRRRLCHICGAFCESTFDLCPVCQTR
                     FDGQNSLLATMLDMPNVRLRRRERITCDEEERRRLGYDIQTAFQFAGSPDKPRVQRAD
                     VFVGTTPVLHLIYAPAATLLRVNHGFRMAKQPGFWVDFESGEVHTKPPQEKNAPPRRS
                     RIQNIRLAVQGTQNMLLVQLVRPELRDDSILETSLQYALQRGCEQLFQLEESELSTER
                     IGKGKHRAILFYEAAEGGAGVLRRLVEEADAISRLAREALAICHFDEQGNDLLPKCQA
                     ACYQCLMSYGNQHEAMQLDRHRILQTLRDLLDSRTLPWTGGRDWNAHLEWLRSLTDSR
                     SELERKLLDALAADHRRLPDEAQQPIPELQCIPDFFYSPNICVFCDGSVHDEPAQAAR
                     DAEIRRELVNRGYRVIVIRYDQDLQSQIAKYPEVFG"
     assembly_gap    4884..4983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4984..5734)
                     /locus_tag="PLD05_14020"
     CDS             complement(<4984..5734)
                     /locus_tag="PLD05_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOP78605.1"
                     /translation="MSRGKSIFDLHSQVLADYRDFVRSFIEIADARAREFVNRALVEQ
                     GDLWPEPLVQLSPAYATGKTVDDLAAEGLIAAETAEIFRQDNGKPFRLYKHQEEAIRK
                     ALAKKNFVVTSGTGSGKSLCYFLPIVDSLVRAPDTGDRVAALVVYPMNALVNSQFQAL
                     NRLKERYEARMGRPFPVTFAKYTGDTAEDVRAQMRRHPPQIMLTNYVMAELLLVRPED
                     QRFLERAMPSPPTPLPEGEGRCPGTLLPEGEG"
     gene            complement(5731..>6653)
                     /locus_tag="PLD05_14025"
     CDS             complement(5731..>6653)
                     /locus_tag="PLD05_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78606.1"
                     /translation="FVRGNERYLPLYEAKMIHQFDHRFGDYHDLPPRSKSTELPEVPL
                     DRLQDPNYRVLPRYWVPEAEVETRLRAKGWDRGWLLGWRRITNVTNERTVIAAVIPRV
                     GVGDKFLLMLPHIPCQAKSLVASCDSCVLDYCARQKVGGTSLKYFTMKQLPFLPPATF
                     DRPCPWSPGETLADFLRPRVLELTYTAWDLEPFARDLGYTGPPFRYDPERRAVLRAEL
                     DAAFFHLYLGSPEEWQREAPASLRELFPTPREAVDYILEQFPIVRQHDEERYGEYRTK
                     RMILEIYDAMQESIRTGIPWQSKLVPGGEG"
BASE COUNT         1281 a         2030 c         1889 g         1353 t
ORIGIN      
        1 ggttcgattc ccgccgcctc cagtcctaaa tctctgcgga atgagcggtt acgagacaaa
       61 gcagagaagt gattaaagtt agggcgaggt ggggaggtcc tttccttatc tgccgcttgc
      121 ctatttcata gctgtctctt gtctatttca gtcgttgggg ctagctactt tggcacaggg
      181 tgcaaccgcg gttgggggct tggccggcag tcttgggctg accgtaaagg cgtgcggtcg
      241 tttgccagga gttggcatcc ttattcgcta aatagcggtc aggctcagcg taacaacttc
      301 cgtcgtttac cagtagtggg cttcggcgat cttttcggct ttacccaacc aggtggtcac
      361 cacgcatcca ggatcagaca ctttgccaga ttcaatgcct atccaaacac ctccgggtat
      421 tttgcaatct gcgattgcag gtcttggtcg tatcgaatga cgatcacacg gtagccgcgg
      481 ttgaccagtt cgcgacggat ttctgcatcc cgtgcggctt gggcgggttc gtcgtggaca
      541 gacccatcgc agaagacgca aatgtttggc gagtagaaga aatccggaat gcattgtaac
      601 tcgggaattg gttgctgggc ctcgtcggga agccgacggt ggtccgcggc cagggcgtcc
      661 agaagcttac gttccaattc tgaacgggaa tcggtcaggg atcgcagcca ctccaagtga
      721 gcgttccagt cacgtccgcc ggtccacggc agcgtccggc tatccaatag gtccctgagg
      781 gtttgaagta tgcgatggcg gtccaactgc atcgcctcat gctggttgcc gtagctcatc
      841 aagcactggt aacatgctgc ctggcatttg gggagaaggt cgttaccttg ttcgtcgaag
      901 tggcaaatag ccagcgcctc ccgagctaga cgcgagatgg catctgcttc ctcaaccaac
      961 cggcggagga cgccggcgcc tccttccgct gcctcataaa agagaatcgc ccggtgcttt
     1021 ccctttccga tccgttcagt ggacagttcc gattcctcaa gctggaagag ctgctcgcaa
     1081 ccccgttgca gggcatattg cagggatgtt tccaagatcg aatcgtcgcg caattcgggt
     1141 cgcacgagtt gcacgagcag catgttttgc gttccctgca cagcgagccg gatattctgg
     1201 atccggctac gtcgcggagg ggcattcttt tcctgcgggg gtttcgtgtg tacctcgccg
     1261 ctttcgaagt ccacccagaa acctggctgc ttggccatcc gaaagccgtg gttcacgcgg
     1321 agcaacgtgg ccgctggtgc gtaaatcagg tggaggaccg gggtggtgcc gacgaagacg
     1381 tccgctcttt gcacccgggg tttgtctggg ctacccgcaa actggaatgc agtttggatg
     1441 tcgtagccga gccgacgccg ctcttcctcg tcgcaggtga tgcgttcgcg ccgcctcaac
     1501 ctcacattgg gcatgtccag catggtcgcc agcaggctat tctggccgtc aaaccgggtt
     1561 tggcaaacgg gacacaggtc aaacgtactc tcgcaaaagg caccacagat gtggcatagc
     1621 cggcgccgga ccagacgctc atcgagccct cccggcgggg actgcaaact gacgacctcc
     1681 cacttggtcc cctcatgata gagaatgtta ttcggcgcca actcgcggat ggcaagaaaa
     1741 cgcggacggc caataaattc gccttcatcc ctaggtaccc atgcccgcac cggcaaggcc
     1801 gggaagttgt agcccggcag gaacccctcg ctcgccaaat atcggtacgg ataaaagtcg
     1861 ccttcctctc gcgcgacatc aatttgacac aacaggttga gttgccgctg ggcttccttc
     1921 atcttgcgat cggctttatc ctggtcttct attgtgcgcg cccgcttaag ctcgttatag
     1981 gccgcctctc gctgctgcac ggccgcccgg taaagttctc gccaccgatc gaacgcccga
     2041 tcgaattgct gcggtgcttc gtccaggacg cggtcaatcc aatcctcgtt aaaccagccc
     2101 gtttgttcca gaattcccat atccggtgcc aagacgttct gcacccgacg gcgcagttcg
     2161 tatctcgctg cctcaccgag ctgaatcgcc ccggctacct ccgttttgag gggcaaatcg
     2221 tcctgttccc tgtcgatcac gtcctcaatg gactgcccca aaggcaggcg aacttgggcg
     2281 agccacatgg cgtgcacgtg ggcccgcagg agggcttcat tggtcaaatc caggcgtggc
     2341 ggtcggacgc taccagcaac catctcatcc cggcggttaa agaagtactg gtcgtgactg
     2401 ttcagggcac cgcaataggt aaatatcagc cctggttgac cctgacgtcc tgcccgccca
     2461 ctccgttggg cataattggc aggggtcggc ggaacatttc gcatgtggac gaagtcaaga
     2521 tctgcaatat cgacgccaag ctccatcgtc ggcgagcaca ccaaataggg caggcggcgt
     2581 cccacctctc tttccttctg cgtatcgctc tcttcccacc ggaaacggcg ttcgcggcgc
     2641 tcgcgctcgc ccggcctgac cacctgggcc gtgtgttcac gggcctcaag tcccgctaaa
     2701 aagcccggcc actcctgata gaagcgctgg aaaaaagcgt tgaccggcgg cacacgacgt
     2761 gaatacatcg ggtcgggcgg cggcgttcca tctcccagtc gccacaccaa acaggccgcg
     2821 tcgagctgat agaactggtg gtcgtccttt acttcacgaa ccagaaaacc ttgttccacc
     2881 agcagtttca gcagggcatc cagaaacgtc agatactcgt ccgtggaaag gccaagccgt
     2941 tgtcggagga acctgccaat tgcactgcgt tcgcccagcc tgtatcccag cgccaactgc
     3001 ctcgactgtc ctagtcgcac gtatcgcgcg gcggggcaaa gttcgttggc gtccgggtca
     3061 agcccccaga actcgttgag ttgttgttcg gcccgtttgc ggatctgctg ctgctcggtt
     3121 tcccgaagac aatcacaccg gatcgccagt ttgcggcgga actgatccag cacggcccgg
     3181 acaatggttt cccgctctgc cggtgacaac cgcgcgacgt tggggtgaaa ctgccagtat
     3241 ttctcaccag cgcacaggtc ctctagcccg cgatacccaa tccgtaaaag cccgacatgc
     3301 tccaggttcg gttggaccac acgccacccc cgacggagat cctcgtagag acggtattcc
     3361 gtcagctcag tgaagacctt ccacacatcc cgggctgcgg ccgattctgg ggccagttcg
     3421 ggatttcggg caatgtctcg gatcgtcaga ccgctgcact cgaccacgtg tttggctacc
     3481 tcagagaagg tcagctccga cttttgcttc agggctgcat acaacgcaca acgaatcagc
     3541 gagacgtgga cgaaatcgtt gaaatgcccg gcctgaaggg aagcgtcctg gcgattgtcg
     3601 gtgaagctca gcagcttatc gcgcggcccg tcgccaccgc tggcgtgacg caaaagagat
     3661 acagccagca cggtggttgc gctcgagcgt ccttcgctgg agagcgacgc caacttggcg
     3721 aagtctcgct ccttggccgt gtagaattcg ccacaacgca gacacagcga gaacggttcc
     3781 ttttgccacc acattttgat cgccccagcc cgagaatttc tccagtacgt gccgtccggg
     3841 gcgacccaga ccgcggtggg cactcggtcg cgccaggaat tcttaaatcg accgctggca
     3901 tcgtaccatt cctcgggaat gcggtcctcg ctccaatcct cctcggcagg cgccagcatc
     3961 aagtagcccg cttgctgttc ctcctcgtcc tggccaagct cgatgccgat gggatgcggc
     4021 aggaagcagc cctcaccccc tacccctctc ccgaagggcg agggggaagt cccctcaccc
     4081 cctacccctc tcccagaggg agagggggga ttggtttgca gcacgtgata atagtcctgg
     4141 ccgcattggc ggcagaactt aacggggacg aagattcggc cacccccagc ttgagtttga
     4201 ccctccaacg agaattcacg ttgtggtgcc ggttccaacg tggcaaacag tgcccggcct
     4261 tggctgatga attggtgcaa cttaaaggcc agggcccgat tgccgccttc tcccggcatg
     4321 gccccgccca gttccaggac ctgtcgcaag tattcttggc atcggtgccg cgcgatgccc
     4381 gtcaattccg caagttcatc cgctgcccct gaaagcgtcc ttggaacacg ccgcctaaga
     4441 ttgccctcgg cgtccttttc gatccccagg gcgtattcca cccagcgcac cagcggatgt
     4501 ctgcgaaaac tctcggcatc cttttgccaa tccccgtcgg tcattgcctt tcggagctcc
     4561 tccacggtcg gcgggccgcc ttgggtgaac ggcaccagcg tttcctcgat gacgtcctcg
     4621 gcctggaagg aatggccaaa caaccgcccc gcaaagtccg ccaccgcggc acgacgctcg
     4681 gcaggcgtgg cctggcggct ggccaccatc gtggcactgg tccctatatg gacaaggtca
     4741 cgggccgcac agcgttcttt cagcctgcgg atgagcatcg ccacgtcggc cccctggcgg
     4801 ccgcgataag tgtgcaattc atcgaacacc aaaaaccgca gcccgccacg cgctctcccc
     4861 tctccctctg ggagaagcgt tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnntcccctc tccctctggg agaagcgttc ctggacacct cccctctccc tctgggagag
     5041 gggttggggg tgagggcatt gcccgctcca aaaagcgctg atcctcgggc cgcaccagca
     5101 gcaattcggc catcacgtag ttggtcagca tgatctgggg tggatggcgc cgcatttggg
     5161 cgcggacatc ctctgcggta tcgccggtgt actttgcgaa cgtgaccggg aatggccgtc
     5221 ccatgcgggc ctcgtaccgc tccttgagcc ggttgagggc ctgaaactgc gagttcacca
     5281 aggcattcat gggatagacc accaaagcgg ccacgcggtc gccggtatcc ggtgcgcgga
     5341 ccaggctgtc cacaatgggc agaaaatagc aaaggctctt tcctgaacct gtgccgctgg
     5401 tgacgacaaa attctttttc gccagggctt tgcggatggc ctcctcttga tgcttgtaca
     5461 agcggaaggg ttttccgttg tcttgccgaa agatttcggc ggtctcggca gcaatcagac
     5521 cctcggcggc caagtcgtcc accgttttgc ccgtggcata ggcgggactc agttgcacca
     5581 gcggttcggg ccaaaggtca ccctgctcca ccaaggcccg gttcacaaat tcccgcgccc
     5641 gagcgtcggc aatctcgatg aatgaacgaa cgaaatcgcg atagtcggca aggacttgcg
     5701 aatgaaggtc gaagatactt tttcccctgc tcatccttca cctcccggta ccagtttgga
     5761 ttgccacgga atccccgtcc gtatcgattc ttgcatggcg tcgtagattt ccaggatcat
     5821 ccgcttggtg cgatattcgc cgtagcgctc ttcgtcgtgc tgccggacaa tggggaactg
     5881 ctcgaggata taatccaccg cctcgcgcgg cgtggggaac agctcgcgga ggctggctgg
     5941 ggcctcccgc tgccactcct cggggctgcc aagatacagg tggaagaacg cggcatccag
     6001 ttcggcccgc agcaccgccc gacgctcagg gtcatagcgg aaaggcggac cagtgtagcc
     6061 caggtcgcgg gcgaaaggct ccaggtccca ggccgtgtag gtcagttcca gcacccgcgg
     6121 acgcaaaaag tcggccaacg tctcgcccgg cgaccagggg cacgggcggt cgaatgtagc
     6181 gggagggaga aacggcagct gcttcatggt gaaatatttt agggaagtcc cccctacttt
     6241 ctgacgcgca caataatcaa ggacacaaga atcacaactt gcgactaagg atttggcttg
     6301 acatgggatg tgtgggagca ttagaagaaa cttgtcgcct acacccacgc gcggaataac
     6361 agcggcgatg acggtacgtt cgtttgtcac gttcgtaatg cgtcgccagc ccagaagcca
     6421 gccgcgatcc cagcccttgg cccgcaggcg ggtttcgacc tcggcctctg gtacccagta
     6481 gcgggggagc acccggtagt tgggatcctg aaggcggtcc agcggtactt cgggcaactc
     6541 cgtgctcttg gaacgcggcg gcaggtcgtg gtaatcccca aagcgatggt cgaactggtg
     6601 gatcatcttg gcctcgtaca gcggcaaata gcgctcgttt ccccggacaa aga
//
LOCUS       DAOSUT010000200         6582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5595_length_6582_cov_9.621112, whole genome shotgun sequence.
ACCESSION   DAOSUT010000200 DAOSUT010000000
VERSION     DAOSUT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_6582_cov_9.621112"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..646
                     /locus_tag="PLD05_14030"
     CDS             65..646
                     /locus_tag="PLD05_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78607.1"
                     /translation="MRVSDLGLPATAIAPVVTRNNGKTNQGQQISEGTTSQRLQPPEG
                     DQSPKPLHRLAQVRIRQGVSRRTMARRMKVDIRTVREEEDPTRDLPLSALYRWQRALQ
                     VPLIELLSDIEEPLSAPVLKRARLLRMMKTVRAIQLRAKQPVVRQLAELLAEQLLEIM
                     PELADVSPWPMGRRPRTSDCGPTADRIFKVRFR"
     gene            826..2262
                     /locus_tag="PLD05_14035"
     CDS             826..2262
                     /locus_tag="PLD05_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78608.1"
                     /translation="MECQDSRQEATRQRRCDGWWKSARTSQGGERLGRVIPWRLAIFG
                     LCLCLVVAGCRQASQPVAPSPSPLEELGRLPGLRRSTHPQLSEELARIEEEGGTPEQL
                     TVPLPPREDNAASALVALFPKERVEFLVAQTSDWLRVAPDRLQKEELQAAKSLLQSFQ
                     TQRATCRAALLRPKCVFPIRFTAGFGADLSFVDQCVLAVQIEHLAAALAISEGNLDAA
                     VDALNVALRLCGVLSRTKHPLLRWQAASLRRQTLDCLPTLVSHPEITRDALGQIADTL
                     QKELAIWPSDAETWIGARALGMHAYELVRIGRLYDVLTEREIKQLRQSYDLEELVRTT
                     QEHADQDEFYYLTQMREIIKHSQKPYCERAGELEKLQAAWEHKSHDDETVVACRVLLP
                     EVPTGQKTQAEDLANVCAWLVALRAAREDIQSWDTVNPLTGRPFIVEVQDDTVYVSGI
                     GTDIGLHGAIGVPRRPPPKGSQPKREQT"
     gene            complement(2401..2718)
                     /locus_tag="PLD05_14040"
     CDS             complement(2401..2718)
                     /locus_tag="PLD05_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78609.1"
                     /translation="MTKTAVIQAQQRWEYVFIQRRSELALQDELNVMGQQGWELVHVE
                     YYKDAKGIMGWIAFLKRPSAASKPPGTGAGEHEVMHEKEPTPGGVQRPMPPPDDVFEV
                     KTE"
     gene            complement(3147..4592)
                     /locus_tag="PLD05_14045"
     CDS             complement(3147..4592)
                     /locus_tag="PLD05_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOP78610.1"
                     /translation="MSESLFGIIVLLSFICQIAAAAVGLRIAKISRPGWGWLLLAGGM
                     VLMAIRQGVVLAAWWRGFSGDFSQETIALFVSIACLLGVLLIKPVLDASWRFQTFMEM
                     ETERLNAILQLLPGMVYRCRNDPQWTMEFVSDGCRELTGYTPEDLIGNRRIAYGEVII
                     PEDRDAVYREVEAAVANKRPYRLVYRIRTAQGQVKWVWEQGAGVFDEEGEVLYLTGYI
                     TDVTVWRNIEEELRKAQATWAQEKEEWVKQEGQLLSRVWHTFEHQNQTIAYEIHDGCV
                     QLATAALMNLESYRHQRATSPETAEQLLERAIQLLRDSIAEARRLIQGLRPVALEEQG
                     LIPALQMLFKDWEKRTNVQIHFVNRTDIARLVPPVEVNVYRIIQEAVQNAIRHSQSPE
                     VFVQLAKEGDQLVIEVRDEGVGFDVKSVPRERFGLTSIRDRARLLGGWADIVSEFGRG
                     TLVRVVIPLNPLVSAPTNNGSQPLGPVPVNS"
     gene            5014..6327
                     /locus_tag="PLD05_14050"
     CDS             5014..6327
                     /locus_tag="PLD05_14050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOP78611.1"
                     /translation="MRIAIVGTGYVGLVTGTCFAETGNDVICVDCDEAKINRLQKGEC
                     PIYEPGLQELLHRNITAGRLQFTTDIATATRWARLIFIAVGTPPREDGSVDLTALWNV
                     VEAIAPHLKPDAIVVIKSTAPVGTNAAVTACLRELTGRTVNVVTNPEFLKEGAAVEDF
                     MKPDRVVVGTRSEEVFEVLWELYQPYLRTDKPILCMSPESAEMTKYAANAMLATKISF
                     INEIAAICERTGADINDVRRGIGHDSRIGFAFLFPGVGYGGSCFPKDVRALAAVARAH
                     DVVPRILEATHAVNEHQKGILLRKIREHFGGALSDKTIAVWGLAFKPGTDDIREAPSL
                     VVLDGLLKHGATIRVYDPEAMENVRRIYGNRLIYCAQRDDALNDADALVILTEWKQFV
                     HPDFEVMKSRLRRPVVFDGRNIYNPYRMRDLGFTYYSIGRPPVRP"
BASE COUNT         1447 a         1891 c         1824 g         1420 t
ORIGIN      
        1 cgattcctct tccggtcttt ggcggtcttt tgccctgtct ttttggggct aaaggaggaa
       61 gaccatgcgc gtctcggatt tgggtttgcc ggccactgcg attgccccag tggtcacacg
      121 taacaacggc aaaacgaacc aagggcaaca gatcagcgag ggtacgacca gccaacgttt
      181 acaaccccct gaaggagacc aatcgccgaa gcctcttcac cggttggcac aagtgcggat
      241 acgtcagggg gtttctcgtc gcaccatggc ccgtcggatg aaggtcgata tccgcaccgt
      301 ccgagaagaa gaggatccga cgcgagacct tcccctttcg gcactatatc gctggcaaag
      361 ggccttgcag gttcccctga ttgaactgct ttccgacatc gaggaaccgc tgtcggcgcc
      421 ggtcctcaaa cgtgcccgtt tgctgcgaat gatgaagacg gtgcgggcga ttcagcttcg
      481 ggcaaaacag cctgtcgtgc ggcaattggc cgaacttctg gcagaacaat tacttgaaat
      541 catgcccgaa ctggccgatg tcagcccgtg gccaatgggc cgccgtccac gaaccagcga
      601 ctgtggcccc acggcggatc gcattttcaa ggtccggttt cggtaatcgg caattagcag
      661 cttgatacgg cccgcggtgt gcccacgctt tggggaaagg cgccgtgtca gtttagtcaa
      721 actgtgcgcg cagtatcgga actttctcgg ttcctaaaag gtgatatcat acaacgagag
      781 tggtgatcgt ttgatccacg agaccacctt gaaggagccg agttgatgga gtgtcaggac
      841 tcacggcagg aagcgacgcg ccaacgtcgg tgcgatggtt ggtggaagtc cgcgagaacg
      901 tcccaaggcg gtgaacgtct cgggcgtgtt attccgtggc gattggcgat tttcggtttg
      961 tgtctttgtc tcgttgtcgc gggatgccgt caggccagcc agccggtggc gccttcgccg
     1021 tctccacttg aggagctggg acgcctgccc ggcctacgcc gttcgaccca tcctcaattg
     1081 agcgaggaat tggcccgcat tgaggaagaa ggcggtacgc ctgagcagtt gaccgttcct
     1141 ttgcccccgc gcgaagacaa tgctgcctcg gcacttgtcg cattatttcc gaaagagcga
     1201 gtggagttcc tggtggctca gacaagcgat tggcttcggg tggcaccgga tcgtcttcag
     1261 aaagaagagc ttcaagccgc taaatcgctg ttacaatcat ttcagacgca gcgggcaaca
     1321 tgccgcgcag ccttgttgcg tccgaagtgt gtctttccca tccgctttac ggccggattt
     1381 ggtgccgatc tgagctttgt cgatcaatgc gtcttggccg tgcagattga gcacctagcc
     1441 gccgctttgg ccatctccga gggcaatctg gatgcggccg tggatgcgct caacgtggcc
     1501 cttcgcctgt gtggtgtcct ctctcgaaca aaacacccgc tgctgcggtg gcaagccgcg
     1561 tccttacgga gacaaacgct cgattgcctt ccgacgctcg tgtcgcaccc cgagatcaca
     1621 cgggatgcgc tagggcaaat tgccgacaca ctgcaaaagg agctcgcgat ttggcccagc
     1681 gatgcagaga cctggattgg tgcccgagct ttgggaatgc atgcctacga gttggttcgc
     1741 atcggtcggc tgtatgatgt tctcacggag cgcgagatca aacaattgcg ccaaagctac
     1801 gatttggaag agttagtgag aaccacacag gaacatgccg accaagacga gttttactac
     1861 ttgacccaaa tgcgcgagat tatcaagcat tcccagaagc cttactgcga aagggccggc
     1921 gagcttgaaa aacttcaggc agcctgggag cataagtccc acgatgacga aacggttgtg
     1981 gcgtgtcgcg tcttgcttcc ggaggttccc acgggccaga agacccaggc cgaggacctc
     2041 gccaatgtat gcgcgtggtt ggtggcgttg cgagcggccc gggaagacat ccagagttgg
     2101 gacaccgtta acccgctgac aggtcgcccg tttatcgtag aagtgcaaga cgacaccgtt
     2161 tacgtgagtg ggatcggcac ggacattggc ctgcatggtg ctatcggagt cccccgacgc
     2221 ccaccgccca aaggaagcca gcctaaacgc gagcaaacat gatccagtca cccaggtctc
     2281 cgcccgaatt gccaccgcaa gtgcggatcg tgggaatgtt gtgacggctg gccgtatcgt
     2341 ctcctgtcga gcacggaacg accaaatcga ttcggcctaa aggcatcaca tgtggggcgc
     2401 ttactccgtt ttgacctcaa acacgtcgtc agggggtggc atggggcgtt gcacaccacc
     2461 tggggttggc tccttttcgt gcatgacttc gtgttcgccg gcacccgtgc ccggtggctt
     2521 gctggccgcg ctggggcgct tcaaaaaggc aatccagccc atgattccct tcgcatcctt
     2581 gtaatactca acatgcacta actcccatcc ctgctgcccc atgacgttta attcgtcctg
     2641 aagggccagc tccgatcgtc gctggatgaa cacatactcc cagcgctgct gcgcctgaat
     2701 tacggcagtc ttggtcatgc tccacctcct tttcctcccc acacccgccg aaaaaaccaa
     2761 tgaccacgct aagacggcgt tccgcggtgc aatgaacccg gccgtcgtac gaggcaatac
     2821 cctggcgcgc cgtcaaatgt atcgcgccgc tgtcaaaaat gcaaaggttt tgttgccgct
     2881 tttggaactt cttgagagcc gtttagaccg ccagcagaag aatatctcca tacgtcccga
     2941 cgtccaggca aacaaaactg gcactttaga acctatccca aagttccctc tccctctggg
     3001 agagggttgg ggtgagggca agccctcgtc ggagtcgatc gaaaaatcca gcaacgattc
     3061 gagccatgct cacctcggtc tctctcccgc cgaacgggca aggggaggtt gtcggatagg
     3121 ctcttagctg ttatcagaag gaacgatcac gaattgacgg gcacgggccc gaggggctgc
     3181 gagccgttgt tggttggcgc actgacgagt ggattgagtg ggatgaccac acgcacaagc
     3241 gtcccccggc cgaactccga gacaatatca gcccagccgc cgaggagtct tgctcggtcc
     3301 cgaatggacg tcaatccaaa gcgctcccgg ggaacgctct tgacgtcgaa tcccacaccc
     3361 tcgtctcgaa cctcgatgac cagttgatcg ccctctttcg caagctgaac aaagacctcg
     3421 ggagactggc tatggcgaat ggcgttttgg acggcttcct gaatgattcg atagacgttc
     3481 acttcaaccg gcgggaccag ccgcgcaata tcagttcgat tgacgaaatg aatctggaca
     3541 ttggtccgtt tttcccaatc cttaaacagc atttgcaggg ctggaatcag cccctgttct
     3601 tccagggcaa cgggtcgcag tccctgaatc aatcgccggg cttccgcaat ggaatcccga
     3661 agcagttgaa tagcgcgctc gaggagttgc tcggcggtct ctggcgacgt cgcccgctgg
     3721 tgccgatatg actcgagatt cataagggcg gccgtggcca attgaacgca tccatcatga
     3781 atctcgtaag caatcgtttg attttgatgc tcgaacgtat gccaaacgcg gctgagtaat
     3841 tggccttctt gtttaaccca ctcctctttc tcttgtgccc aggttgcctg ggccttccgc
     3901 aattcctcct cgatgtttcg ccatacggtc acatccgtga tgtagcccgt cagatagagg
     3961 acctctccct cctcatcgaa gacccccgca ccttgttccc aaacccattt aacttgtccc
     4021 tgggcagttc gaatccgata gacaagtcgg tatggccgtt tgttggcaac agcggcttca
     4081 acctctcgat aaacagcgtc gcggtcttcg ggaatgatga cctctccgta ggcgatgcgc
     4141 cggttgccaa tgagatcctc cggcgtgtag ccggtcaact ctcgacatcc atcgctgaca
     4201 aactccatcg tccattgtgg gtcattgcgg caacggtaca ccatgccggg caggagctgc
     4261 aggatggcat tgagccgctc cgtctccatt tccatgaagg tttgaaaacg ccaagaggca
     4321 tccagcaccg gcttgattaa gagaaccccc aaaaggcatg caatcgatac gaataaggcg
     4381 atcgtttcct gagaaaaatc cccggaaaag ccacgccacc atgcggcaag tacgacgccc
     4441 tgacgaatcg ccataagcac cataccaccc gctaatagga gccagcccca gcccggacgg
     4501 ctaatcttgg cgatgcgcag gccaaccgcc gctgccgcaa tctggcagat aaaagacagc
     4561 aaaacgatga ttccaaaaag cgattccgac acggtccttc aacctttcca accccgtttt
     4621 ctccctctct tgaaatttga gcttatttcg ggcagtgagg caaattacaa aaaccgtgcg
     4681 gaggatggaa attgcttatg cgaactcgcg ctctacagcc ctttctcggc gataaaccga
     4741 cctgcgccta ttccagagac cttcgcagaa cattggttac tgcagatgca aagcagaagc
     4801 tgactctaca agtactttac ggagtctcac acatcggtca ataacccgct ggagggggta
     4861 tcgtacctgg tccgccggca ggttccacgt gtaccagatt agtcacatgc tatgattgga
     4921 tacgacatcc ccgaaacgct cttgcttgag gaacgtcggc ctcgaaccta cctcgaccta
     4981 ccgctcgtgt acgctagaca aggagacgct gtcgtgcgta tcgccattgt cggtactgga
     5041 tacgtcggcc tggtgacagg gacctgcttt gccgagacag gcaacgacgt catctgtgtg
     5101 gattgcgacg aagcaaaaat caatcgcttg caaaaaggcg agtgccccat ctacgaacca
     5161 ggcttgcaag aacttttgca tcgaaacata acggcgggac ggctccaatt cacaacagat
     5221 atcgccacgg caacccgttg ggcgaggttg atcttcattg ccgtgggtac gccacctcgc
     5281 gaggacggct ccgtcgatct cacggcactt tggaatgtcg tcgaggctat cgctccccat
     5341 ctcaaacccg acgcgatcgt cgtcattaag agcacagcgc cggtggggac aaatgcggcg
     5401 gtgacggcat gcctgagaga attaaccgga cgtactgtca atgtggtcac gaatcccgaa
     5461 tttcttaaag aaggggcagc cgtggaagac tttatgaaac ccgaccgagt ggtcgtcgga
     5521 acacggtccg aagaagtctt cgaggtcctc tgggaactct atcagccgta tttgcggacc
     5581 gataaaccga ttctttgtat gtcccctgaa agtgcagaaa tgaccaagta tgcggccaat
     5641 gccatgttgg ccaccaagat tagcttcatc aacgagatcg cggctatctg cgaacggaca
     5701 ggggccgata tcaacgacgt ccggcggggc attggtcatg atagccgcat tgggtttgca
     5761 ttcctcttcc ccggcgttgg ttacggggga agttgctttc ccaaggatgt acgcgccctg
     5821 gccgcagtgg caagagcgca tgatgtggtg ccccgaattc tggaggccac gcatgccgtt
     5881 aacgagcacc aaaaaggtat cttgctgcgg aaaatccgcg agcatttcgg cggtgccttg
     5941 tccgacaaaa cgatcgccgt ctggggattg gcctttaagc cgggaacgga cgacatccgt
     6001 gaggccccgt cgcttgtcgt tctcgacggg cttttgaagc atggggcaac catccgcgtt
     6061 tacgacccag aggccatgga gaacgtgcgt cgcatctatg gcaaccgtct aatctattgt
     6121 gcgcaacgag acgatgccct caacgacgcc gatgccctgg tgattctgac ggagtggaag
     6181 cagttcgtac atcccgattt cgaggtcatg aaatcccgcc tccgtcgccc cgtcgtgttt
     6241 gatgggcgga atatctacaa tccctatcgc atgagggatt taggattcac ctattacagc
     6301 atcggccgtc cgcctgtgcg cccatgacac ttgtgtagaa tatccgcgcc tcgggcgtag
     6361 gctgccggtg aaacttcttg agcctttggc gtaagccgga ggtgaaacca tcaaatgcag
     6421 tgattgcaca atcctggccg ggatgtgccc tacttgatat aatggggcct aaaggttggt
     6481 caacatgaaa cggcttgttt tcgccccatg cctcgcagac gggtaaacat accgctgatc
     6541 ctcgttacca cggtgtggta cgccctgaca gccgtgatgg gt
//
LOCUS       DAOSUT010000201         6533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5636_length_6533_cov_7.634764, whole genome shotgun sequence.
ACCESSION   DAOSUT010000201 DAOSUT010000000
VERSION     DAOSUT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_6533_cov_7.634764"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5243)
                     /locus_tag="PLD05_14055"
     CDS             complement(<1..5243)
                     /locus_tag="PLD05_14055"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HOP78612.1"
                     /translation="MLRIKTWLPLVALTLLVYAPSKYPTAQVTDAVELPRAGDGQWRL
                     NPNSSPEDIYRQVANGATAYLRSQVPLEWRQMWSAQRALAEAEALGKRVSQLEGVADR
                     MPVIGVGRACRMFEPWNRLTVNGEAVPHETDAAPSPRGGPDNEVVVGTGELLLEETDI
                     SLPDRGGLGLSFVRYYSSHVGYDGPLGPGWDHNCNQRIVAEGNPSKPTALIWYTGRRP
                     IRFEWKNGTWEPEPGAFYQLTLNDNQITIETPLRVRLTFTATSSSVGGGRWRIASIAS
                     RHDQWRANVIRFTYWPGSDRLQVVEDAYGNRLQFGYDPSGKLTGVVCGQLAVLYQYDE
                     LGRLTRVVLPRVATELTKTADVTWEYRYGTGLDGRTRLVAIIPPKGEPEKHYSYETDS
                     NAPAYGRVGGIVLRGAGDTPSVVEWRFETIHKEKTWTVIYKQPLPLPEERWTFPEIGR
                     WPGCYPLCREIPSQNATWSWVHNAAGQKLEARSPLGQVTRWEYASDHRDPRFRGNLLA
                     KREKARPGSGLLIVERGWEWTYHKDIALPIEAVVYQIENGGAKRVLLRQSYEYDPTNL
                     EVSIERVGDTETRIVRNRYGLPVVSWDGRGCATVYRYYSRFNEGLASEGNGGLLAETI
                     EDVPQTTLTQALRVAGVSLSRDERLPERQAEGKPCQRTSRYGYDGLGRRVKEIYPSYT
                     VYHLLNKRGLRLALLDTRSDLTVYDYDAALRLVAQWQRFTPLPLAVYRGEQRVDTPGH
                     FVVQKFRYDSLGRMVAWQPTLERFGIERNEPVEVNYEYYASGVLRKRITPGGTAVEIE
                     YDTASGKPKAFLLIARDAPEEKLILRQAMRYDPEGFLVGYKDARGATYEQKPDGFGRL
                     YAVVRPDGVRTETLRDGLDRPVQERVLSSDGKVLHETQYEYDQAGRLVCSREHRLAKG
                     IDEWLIAEENRYDAEGNVIGRRSYRDDAWHTFAYDGLGRVVRSQSPTGECSEIYYRDD
                     AVCVETIHRVDTRDGQIYKMRFVTLRDEHGNPWCRIPVGHDGLVGLRRAQLTEHDSFG
                     RIVFEAQPDQPGILRVFNTLGSVDRELTRSASNKVLQERWMDYAPDGLLLRRSIANEP
                     LCFFSTGQESLVRVQRLPVPQVYTADYDQFRRLRKEILPDGRVHEYTYGDDSLLRQLV
                     EYDSTKPETKNVIKLDYDILGRLVAVNEGTGRKLQAFDYDAFGNITKADDYAQVNHPV
                     SVGREYDSLGTLLREEFNLPRDFSTLPVISYGHNLRHGRHTLHIEVGQNPWDGWRSLT
                     FEEDPDGRIRRILKDDSEFCRLEYLGENVVSKNFVEQKVTDTITARDSFLEPLEQKIF
                     DTAIANPGQGENANIVYQARYLRDYWGRIEASSAEVPPRKWQWTYCCARDELGNISAE
                     QGHGIFYSDPEELAATRQSLLRVTQQGSSVSGNPPLGAYCVRRHEYDQVGNRVATYEG
                     LPAQRWPEGTNLASIAPPIRDLQASISTGQGVGQHVQSRSEEHVLLAAKPSSATTISH
                     LGDWSQDEPTFALASSQDRQDSVLLARLASYTEDRNVGPVAFARRDSVPLPPVNDRKA
                     LASNRITCTAETTKQPDSASLSRLYNYDPLGRLIEYVSSTSGEPIRWKLEYDPLGRLI
                     AMQGFEVNSSVSLDQANSTPRYSLRFAYDPFGRRIVKRVSRATPNGTSSDLNVTLYRD
                     NRPLLKLSPSSQNPQHWRLVGQYIWRSGTSEVLAFYGEIPEHLSGKWEWVLHQDLAYN
                     VILGSTRRDGKLELSDIASYWG"
     assembly_gap    5360..5459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5460..6302)
                     /locus_tag="PLD05_14060"
     CDS             complement(<5460..6302)
                     /locus_tag="PLD05_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78613.1"
                     /translation="MLELSSKDEWVVYSWRPDENPADPKAVGRHPTILRAVVLPGRPR
                     DERMKHIVNLLETSVKLPSRRGVNPGLWLEIPADWISPRPSSSKPAGKSAKPQSRRWS
                     TGVMIGIFSLIGVLVVAAGGWCLGIFFGLESGKGRNTSDIFPPDVYEQLLRPLGIEMS
                     EKDRTNAASVRLKFREVYCQSWFVNRFTRVFPGGSATLGDKPPEELLSDIEAIFTRLN
                     DHLNRDQNLHSSSHPDCEFVRVNGDVIRKSLESYSEFTKVIKYLPRHATDDAKTSVTK
                     AVNGV"
BASE COUNT         1420 a         1880 c         1639 g         1494 t
ORIGIN      
        1 ccccaatagc tggctatatc tgaaagttcc aatttgccat cgcggcgtgt gctgccaagg
       61 attacgttgt aggctaaatc ttgatgcagc acccattccc attttccaga aagatgttcg
      121 ggaatttctc cgtagaaggc caagacctct gaggttccag aacgccaaat gtactggcca
      181 acgagtcgcc agtgctgcgg attttgcgac gacggagaaa gtttcagcaa tggcctgtta
      241 tcacgataca gcgttacatt gagatcgctg ctggtgccgt tgggagtagc tctcgaaact
      301 cttttcacaa tgcgacgtcc aaagggatca taggcgaaac gcaaggagta cctaggtgtg
      361 ctgttcgcct ggtcaaggct gacagagctg ttcacctcaa agccctgcat ggcgatcaat
      421 cgccccagtg ggtcatattc gagcttccaa cggattggct ctcccgaagt gcttgagaca
      481 tactcaatga gtctccccag agggtcatag ttgtacagcc gagacaaact agcactatcc
      541 ggctgttttg tggtctcggc tgtacacgtg attcggttag aagccagagc cttccggtcg
      601 ttaaccggtg gcaacggtac actgtcgcgc cgggcgaagg ccacgggtcc gacatttcga
      661 tcctcggtat agctcgccaa tcgcgccaag agaaccgaat cctgccggtc ttggctactt
      721 gcgagtgcga aagtaggctc atcctgggac cagtctccca gatgggagat ggttgttgcg
      781 gaggaaggct tcgcagccaa caagacatgt tcctcactgc gcgactggac atgctggccg
      841 acgccctggc cggtagatat gctcgcttga aggtcccgaa tgggtggggc aatcgaagcg
      901 agattcgttc cctcgggcca ccgctgcgcg ggaagaccct cataggttgc gacgcggtta
      961 cctacctggt cgtactcatg ccgccgaaca cagtaggcgc cgaggggggg attaccactg
     1021 acggagcttc cctgttgggt gactcttaag aggctctgtc gtgtggcggc tagctcctca
     1081 ggatctgagt aaaatattcc atgcccttgc tctgcggaga tgttgccaag ctcatcacgt
     1141 gcgcagcaat atgtccactg ccatttgcgg ggtggcacct cggccgatga agcctcaatg
     1201 cgcccccaat agtcgcgtaa ataacgcgcc tggtaaacaa tgttggcgtt ttcgccctgg
     1261 ccaggattgg ctatggccgt atcaaagatt ttctgttcta acggctcgag aaaagagtcc
     1321 ctggccgtaa tagtatcagt aaccttctgc tccacgaagt ttttacttac tacgttttcc
     1381 ccgaggtatt ccagtcggca aaactcggaa tcatccttga gaatgcggcg gattcgaccg
     1441 tcgggatcct cttcaaacgt aagtgaccgc caaccgtccc aagggttctg gccgacttcg
     1501 atatggagag tatgtctccc gtgccgcaaa ttatggccat aagagatgac cggcagcgtt
     1561 gagaaatctc gggggaggtt gaactcctcg cgaaggaggg tacccagact atcgtactct
     1621 cggccgacgg agacaggatg attcacctgc gcgtaatcat ccgctttggt gatatttcca
     1681 aaggcgtcat aatcgaatgc ctggagtttc cttcccgtcc cctcattcac cgcaacgagg
     1741 cgtccgagaa tgtcgtaatc aagctttatc acattttttg tctctggttt tgtcgagtcg
     1801 tactccacca gttgtctcag aagtgagtca tcgccgtagg tatactcgtg aacccgtcca
     1861 tcaggaagga tttccttcct gagacgccgg aattggtcat agtccgcggt gtacacctgt
     1921 gggacgggca gtcgctggac cctcaccaaa gactcttgcc cggtggagaa aaagcaaaga
     1981 ggttcattag caatggagcg ccgaagcagc aaaccgtccg gagcatagtc catccatctt
     2041 tcttggagga ccttgttgct ggcagaccgt gtcagctccc gatccacgct gccgagggtg
     2101 ttaaaaactc gcagtattcc aggctgatcg ggctgggcct caaagacaat ccgtccaaag
     2161 gagtcatgtt cggtcaactg ggcacgccgc aaaccaacga gtccatcgtg cccgactggt
     2221 attctacacc acggattgcc gtgctcatct cgcagtgtga cgaaacgcat tttataaatc
     2281 tgtccatccc gcgtatccac gcgatggatc gtctccacac acacggcgtc atctcggtag
     2341 taaatttccg agcactcccc agtggggctt tgcgatcgaa caacccgtcc cagcccatcg
     2401 taggcaaagg tgtgccatgc gtcgtctcga tatgaccggc ggccgatgac gttaccctcg
     2461 gcatcgtacc ggttttcctc ggcaatcagc cactcatcaa tgcctttggc cagtcgatgt
     2521 tcgcgagaac aaaccaaccg gcctgcttgg tcatactcat actgcgtttc gtgtaagact
     2581 tttccatcag agctgagaac acgctcttgc acaggccgat ctaggccatc ccttaacgtc
     2641 tccgtcctta ccccgtcggg tcgcacaacc gcgtaaagac ggccgaatcc atcaggcttt
     2701 tgctcgtatg tagcgccgcg ggcatcttta tagccaacaa gaaaaccttc aggatcatag
     2761 cgcatcgcct gacgcagaat caacttctct tccggcgcat cgcgcgcaat gaggaggaaa
     2821 gctttaggtt tccccgacgc ggtgtcatat tcaatttcca cagccgttcc gccaggagta
     2881 atcctttttc ttagtactcc tgaagcatag tattcatagt tcacttcaac gggctcattt
     2941 cgttcgatcc caaagcgttc gagtgtgggc tgccacgcta ccatgcgacc gagcgagtcg
     3001 tacctgaatt tttggaccac aaaatggccg ggtgtgtcca cccgttgttc cccacggtaa
     3061 acagccaagg gcaaaggagt aaacctctgc cactgtgcaa cgagccttaa ggccgcatcg
     3121 tagtcataga ccgtgagatc ggagcgggta tcgagaagtg ccaacctcaa tccgcgcttg
     3181 ttgagcaaat ggtaaactgt gtagcttggg tagatctcct tgacgcgccg gcccagccca
     3241 tcatacccgt atcgactcgt tcgttgacac ggtttgcctt cggcctggcg ttccggcaaa
     3301 cgctcatctc tcgacagaga tacccctgcc acacggagag cttgagttaa cgtggtctgc
     3361 gggacatcct caatagtttc ggctaggaga ccaccatttc cttcactggc tagcccttcg
     3421 ttaaaccgag agtaataacg atagactgtg gcacaaccgc gtccatccca actgaccacc
     3481 ggtaatccat accggtttcg gacaattcgt gtttccgtat caccaacgcg ctcaatgcta
     3541 acttccaggt ttgtcggatc gtattcgtaa ctctgacgca acaagacccg cttggcaccc
     3601 ccattttcaa tctggtaaac aacagcctca atgggcaatg caatatcctt gtggtaagtc
     3661 cattcccacc ccctctccac aatcagcaaa ccagatccgg ggcgagcctt ttcccgcttt
     3721 gccagtaaat tgccccgaaa gcgcgggtca cgatgatcgc tagcgtattc ccatcgggtc
     3781 acttgcccga gcggagagcg ggcctcgagc ttctggccgg cggcgttgtg tacccagctc
     3841 catgttgcgt tctgcgaagg aatttcccgg cacaatgggt aacatcctgg ccacctgccg
     3901 atttctggaa aagtccatcg ctcttccgga agggggagag gttgtttgta aatgacggtc
     3961 caggtctttt ccttatgaat ggtttcgaat cgccactcca caaccgaggg cgtgtctcca
     4021 gcaccccgga ggacgatccc tcccacacgt ccgtatgcgg gcgcattcga atcagtctcg
     4081 tacgaatagt gcttctctgg ttctcctttg gggggaataa tggctacgag ccgtgtccgc
     4141 ccgtccagtc cggtgccgta ccgatactcc cacgttacgt ccgctgtctt ggtcaactcg
     4201 gtcgcaactc gcggtaacac aacccgcgtc agccgtccta gttcgtcata ttggtacaag
     4261 acagcaagct ggccgcatac aactccggtg agttttccag acgggtcata tccaaactga
     4321 agacggttac catacgcgtc ctccaccacc tggagtcgat ctgatcccgg ccaatacgta
     4381 aaccgtatga cattcgcccg ccactgatcg tgtcgggatg cgatggacgc gatgcgccaa
     4441 cgccctccac ccacggagct ggacgtagct gtaaacgtta gacgcacgcg gagcggggtt
     4501 tctattgtga tttgattatc gtttaaggta agttggtaga aagcccctgg ttcaggctcc
     4561 caggttccat ttttccattc aaagcgaatc ggccgccgac cagtgtacca aatcagcgct
     4621 gtaggcttac ttggattacc ttccgcaacg attcgctggt tacaattatg gtcccagccg
     4681 gggcccagtg ggccatcgta acccacgtga gaactgtagt atcggacgaa tgacagtccc
     4741 agaccgccgc ggtccggtag tgaaatatcg gtctcctcca aaagcaattc ccctgtgcca
     4801 acgactacct cgttatcggg cccaccacgg ggcgacggtg ctgcgtcagt ttcgtggggg
     4861 actgcctcgc cgtttaccgt caggcgattc cacggctcaa acatgcggca tgctctgccc
     4921 acaccgatga cgggcatccg atcggcaaca ccttccaact gggatactcg cttacccaaa
     4981 gcctcagcct cggccaatgc ccgctgggca ctccacattt gtcgccactc cagcggaact
     5041 tggcttcgca agtaagcggt tgccccgttg gccacttggc ggtagatgtc ctctggggag
     5101 gaattagggt tcagccgcca ctgtccgtcg ccggcccgtg gcaactcgac cgcatctgta
     5161 acctgcgcag tggggtattt gcttggggca tagaccaaca gcgtcaaggc aacgagcgga
     5221 agccacgtct taatacgtaa cattgtcagc cctccttcat atcggattca cccacacttt
     5281 ccgacgcatc gccagaatct tgcgaacccg tcaaccgccg ccaaaaatcg ccgacctttc
     5341 ctcctggtat cgaaaagtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5461 accccattta cggccttggt cacgcttgtt ttggcgtcgt cagtagcgtg ccgcggcaag
     5521 tactttatca cttttgtgaa ttccgaatag ctctccaggc tcttcctaat gacatctcca
     5581 ttaacccgga caaattcgca atccgggtga cttgaagagt gtaaattctg gtcgcggttt
     5641 aaatggtcgt taagtctcgt gaagatggct tcaatgtcac tcaaaagctc ttcgggaggc
     5701 ttgtctccta aggtcgcgct gccgcccgga aatactcgag taaatcgatt aacgaaccag
     5761 gattgacaat acacctcgcg gaacttgagt cttaccgaag ccgcgtttgt acgatctttc
     5821 tctgacatct cgattccgag cggccgcaaa agctgttcgt aaacatctgg aggaaatatg
     5881 tctgaagtgt ttcgcccttt gccactttcc aacccgaaaa agatccccag gcaccaaccg
     5941 cctgcggcga ctacgagaac tccgatgagt gaaaaaatcc ctatcatcac gccagtactc
     6001 catcgcctgc tttgaggttt tgccgacttg ccagcaggtt ttgacgaact tggccgcgga
     6061 ctgatccagt cggccggaat ctctagccaa agccccgggt tcaccccacg ccgggaaggc
     6121 agtttcacag aggtctccag cagattaacg atgtgcttca tcctctcatc ccgtggtcgg
     6181 ccgggaagaa cgaccgcccg cagaatagta gggtgtcgcc ctactgcctt gggatccgcc
     6241 ggattttcat caggtcgcca ggaatacact acccactcat ctttgctaga aagctccagc
     6301 atgccggttt gccccttctg acaatttggg tccgagcgca ggcgctcccc taattttata
     6361 atgacttcat cgatgtcgag gccgttgcag tacgtccatc cttcgccgtc agcgaaccac
     6421 cgatggacca cgacgggaca gcgaaccgac ctcccagact tggcgatagg agaatccgtc
     6481 aacatccctc ttgcccccgt tcaaagagtt tacggatttg gcaacgttgg tgc
//
LOCUS       DAOSUT010000202         6499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5662_length_6499_cov_9.622129, whole genome shotgun sequence.
ACCESSION   DAOSUT010000202 DAOSUT010000000
VERSION     DAOSUT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5662_length_6499_cov_9.622129"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..373
                     /locus_tag="PLD05_14065"
     CDS             158..373
                     /locus_tag="PLD05_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78614.1"
                     /translation="MRNVFDVINEFGHDEDFDPVPTADFLPTDAPAGSRAKLEVLAER
                     VRKGLPLWHPLDRSDYSGLTGAVEPRD"
     gene            complement(536..847)
                     /locus_tag="PLD05_14070"
     CDS             complement(536..847)
                     /locus_tag="PLD05_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78615.1"
                     /translation="MIAKVAADPCPTPHRLRRLIYDVLCESNQLKTSCYPMTETPVFR
                     RGNPCGVLFCLYGPRLVRLTAIWDFAANEVVFYNAAGERYLAIQVPNCSEMGLAGART
                     C"
     gene            complement(1097..5458)
                     /locus_tag="PLD05_14075"
     CDS             complement(1097..5458)
                     /locus_tag="PLD05_14075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78616.1"
                     /translation="MARTVNKKLAVILVGAVVLAIVSGYLLHGFQTRRNARVFLDRAR
                     EAKTQERFDVAVKNYQWYIDLVPNDLDALSEYGSLLADLKAYWPAFTRLEMVLRQDPG
                     RLETRRKLVQVLTAMGRFRDAQEHIEQYLLKEYPDDPELLTELGRCQRGLGQYERATT
                     TFRRVIELKEDYVPAYAQLAGTYRHDLKQPEQGDQWITKMVLTVGDSAEAYLVAASYF
                     YNAQNYEAALKWADEAHKKDPSNLEMLLVAADSARRLGQLDQAEEFAQKAIAASPESP
                     DPYSVYAATQFAKGNADTAINTLEDAASKVKDKGPLYWALASMQIDALRLADAEQTIE
                     KLEAVSYPRAQVEFLRGRLLTAKEQWFLAARKLESVRPDVTDSPDLLKQVDFLLGLCY
                     AQMGNPDQSVASYSRAMTQDPLWPQARLGRAMALLELGRLDEAVNEYRNVVQLGKATP
                     QGVLEFARLLVLSTLSKPPQERNWLEVEQWLDTLEKDDTTKTDAIILRAEILAAEKRL
                     QEAEQLLESHLSQDAKNDSLGLALVALKLQAGDAAEASRLLDQIEKQSGQTLAVLLSR
                     ARVAVRSGDDKRAKQELANIASAAKKLPAEEYQRLLGTLVGYAIQLSDFDNARRWAEE
                     VARLSPNNLKVRLLLFDLALYAQDMAVLSRVLDEIAQIEGQGPIWLYGQAIRLYLESK
                     GENRSLLQQALDYLSRAANLRPNWMRIPALQGLVFDLLGDTGSAINAYLRAVSLGDRS
                     VRTAGRLVQLLYGQQRFLEADQIIRQLEQQQAPFSSELERLASSISLRLEDYERAVEL
                     ARRAVSGSSDYRDHVWFGTVQSVLGLRARAERREKEAQALLAEAEAAFRKGVELAPES
                     VEPWVALVQFYARIGENAKAEQATNEAKEKVKGGIGPLAMGQVYEVLGKLDEAEKYYQ
                     EALAIAPNDLLVVRQVTEFYLRTGRTESAVTQLNRIISGQVQASESDVSWGRRSIAMI
                     LAAQGGYDNLQKGLELIRQNRSAGRTPNIQDLRAEAILLTSHPQRKKRLEGRDLLEKV
                     VKESPQATPDDRFVLAQMYMADGDVVNAARHMRTLLATRGDDPRYLRAFVAFLLDQGQ
                     VAEAEIWQNRLEKVAPDAFPTLALRTRILTIQGRYDEAIQRLREFAEKETNNPPSDSP
                     SELSNDTPSEGSPGTNAEVSEEQLTKVQQLRWALAGERLEFLGRMLERSGKQSEANLF
                     YEEAEKLYRKFVEKNPNARLLLASFLARRGKLNEGLELLEASWESSDVALVRAASQVF
                     MTLPSSDQAIVQRVQTILEKACELHQRPLSLLMLLGDLYGMQGRFAECEAVYREILRR
                     DPKNVIALNNLGAMLAIQGTRLDEAEKLVQTAIDLAGPQVNLIDSQAMVLIARKKPAE
                     ALKVLSDAIEDEPIPILFFRQAQAYQLLNKPELAAQAFEEAVKRGLTKQSLHPIERKI
                     FDGLENWISQAAPTKGS"
     gene            5874..>6499
                     /locus_tag="PLD05_14080"
     CDS             5874..>6499
                     /locus_tag="PLD05_14080"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78617.1"
                     /translation="MSLFHLVKRLLWCPPRHSSPSQLSGAAILCITLIICYTPLTLPV
                     LLGEEPSASVGGSASGSQSKDVASGEVAANQGKGLTRELCAQYLSEGTFEQKIEAITS
                     LVQLRESGIDLILQALQDHDPRVRAKAITALAELAPLDAERVIPQLLVSLEQDDGFVD
                     GQPNWIRASQAIGKYGDKVLPILEKLWQDTDLKKRMIFCIVCYELGPIA"
BASE COUNT         1447 a         1800 c         1668 g         1584 t
ORIGIN      
        1 actaaacgcc caacacgctg agaaaaacct gattcggcta ccgcacgtag tcgatgtttc
       61 gaaaatggtc gtgttgcaac gatcttgcgt tttgtagaga tagtggcagt tttgatacgg
      121 tcctttggtt tcgaagggtt agaaagagag gtgtcccatg cgaaacgttt tcgacgtgat
      181 taacgaattt gggcatgacg aggactttga tccggttcct accgctgatt ttctgccaac
      241 cgatgctccg gcaggttctc gggcgaagct ggaagtcttg gcggagcgcg ttcggaaagg
      301 cttgcccctc tggcatccac tcgatcgaag cgactacagc ggtcttaccg gcgccgttga
      361 gccgcgcgat tgacccgcca tcttgcctcg cgctcccacc gagatccggt tgccaccttg
      421 aaagtgcaac ctgctcgtct cggcggcctt ctttgtgtca gcctcctgcg gggtcgaggt
      481 ctgcgcccag gcgacgtagt tcccgtcaca tagccatccc attacttcgt ttggcttaac
      541 aggtccgggc ccctgccaac cccatttcag agcaattagg gacttgaatc gctaggtatc
      601 gttcaccagc agcattataa aagacgacct cgttggcggc gaaatcccaa atcgctgtca
      661 atcggactag tctcggtccg tacagacaaa agaggacacc gcagggattt ccccgccgga
      721 aaaccggtgt ttccgtcatg gggtagcaag aggtcttaag ctgatttgat tcgcaaagaa
      781 cgtcgtaaat taggcggcgt aagcggtgag gggtcgggca ggggtcggcc gcaactttgg
      841 caatcatcgt gtgctctctt atggagtgca ggattcaaag aagatcggga cgccgtaact
      901 gccgaagata gcgactttct catgtggtat cggccagtgc gggaatgcca cttcaatgct
      961 atgtgctact gacggatacc gcggccagag aggaatgaaa agcggtttcg gttgtaacga
     1021 gccgcaggat ttcttgttct aaaaaacctg caaaacctgg gtaagccctg cgatcgaagc
     1081 ggaatatatc ggcaaatcag gatcccttgg ttggcgccgc ctgagaaatc cagttttcca
     1141 atccgtcaaa gatttttctt tcaattggat gcagcgactg ttttgtcagg ccccgtttta
     1201 cggcttcctc aaatgcctga gcggccaatt cgggtttgtt cagcagttga tacgcctggg
     1261 cctggcggaa aaagagaatc ggaatcggct cgtcttcaat cgcatcgctt aagactttca
     1321 aagcctcggc tggtttcttc cgggctataa ggaccatggc ctgcgaatcg attaaattaa
     1381 cctgtggtcc tgccaaatca atggctgttt ggaccaactt ttcggcctcg tccagtcgcg
     1441 ttccctggat tgctagcatg gccccgagat tattcaaggc gattacattc ttggggtcgc
     1501 gccggaggat ttcccgatag acagcctcac attccgcaaa ccgcccctgc atcccgtata
     1561 aatcacccaa caacattaac aacgacaatg gtcgttgatg gagttcgcaa gccttttcaa
     1621 ggatggtttg aaccctctgt acgatagcct gatcacttga cggaagtgtc ataaacacct
     1681 gcgatgccgc ccgcacaagc gccacatccg acgattccca agaggcttcc aaaagctcga
     1741 gcccctcatt gagtttgccg cggcgggcaa ggaaactcgc caaaagaagg cgcgcattag
     1801 ggttcttctc cacgaacttg cgatataact tctcggcttc ttcatagaag aggttcgctt
     1861 ccgattgctt gcccgaccgt tcgagcatcc gccccagaaa ttccaacctc tcccccgcca
     1921 gtgcccaacg gagttgctga acctttgtca actgttcttc agaaacctcc gcgttagtac
     1981 ccggagaacc ttctgaggga gtgtcgttcg acaactctga cggcgaatca ctaggtggat
     2041 tatttgtctc cttttctgca aactcccgca gccgttgaat cgcctcatcg tatcgtccct
     2101 ggatcgtgag gatccgggta cgcaaagcca gtgtggggaa ggcatccgga gcaactttct
     2161 ctagccggtt ttgccaaatt tctgcttccg ccacttgtcc ttggtcgagc aaaaaggcaa
     2221 caaatgctct caagtatcgg gggtcatcac cccgggtggc caacagggtc cgcatgtgtc
     2281 tggctgcatt cacgacgtcc ccatcggcca tgtacatctg agctaagaca aaccggtcgt
     2341 cgggggttgc ttgcggcgat tctttcacga ctttttccag caggtctcgt ccttccaatc
     2401 gtttctttct ttgagggtgg gaagtcaaaa ggattgcttc ggctcgcaaa tcttggatgt
     2461 tcggtgttcg ccctgcgctt cggttttgtc ggatcaattc caggcctttt tgaagattgt
     2521 cgtacccccc ttgggcggcc aagatcatag cgatactacg acgtccccaa gagacatcac
     2581 tttcagaggc ttggacctga ccactgatga tccggttcaa ttgggtgacg gctgattcgg
     2641 ttcgccctgt ccgcaggtag aactctgtca cctggcggac caccagtaaa tcgtttggag
     2701 caatggcgag ggcctcttga taatacttct cggcttcgtc cagtttgcct aaaacttcgt
     2761 acacttgccc catcgcaagt ggcccaatac cacccttcac tttttctttc gcttcatttg
     2821 tggcctgctc cgcttttgca ttctcaccta ttcgggcgta gaactgcaca agggcgaccc
     2881 acggctcaac cgattccggc gccagctcga caccctttct aaacgccgct tctgcttctg
     2941 ctaacaatgc ttgcgcttcc ttctcacgac gttccgcacg cgcccgcaat cccagcacgc
     3001 tttgcactgt tccaaaccaa acgtgatctc ggtaatctga cgagccgctt accgcccgac
     3061 gggcaagttc gactgcccgc tcatagtcct ccaaacggag cgaaatgctg gaggcaagcc
     3121 gctctaactc actggaaaag ggtgcttgct gctgttccaa ctggcggatg atttggtctg
     3181 cttcaaggaa gcgctgctgc ccgtagagaa gttgcacaag ccgtcctgcc gtgcgaacac
     3241 tccggtcacc cagactaacg gcacgaagat aggcattgat tgccgatccc gtatcaccca
     3301 gcaaatcgaa aaccaggcct tgcagggcgg gaatgcgcat ccagtttggt cggaggttcg
     3361 ccgcccgcga aaggtaatcg agggcttgct gcaagagact ccggttctca cctttcgatt
     3421 ccaagtacaa acgaatggcc tgaccgtaga gccaaattgg cccttggccc tcaatctggg
     3481 caatctcgtc gagaactcgg ctaagaacgg ccatgtcctg agcataaagg gccaagtcaa
     3541 agagtagcag gcgaaccttc aagttgttgg gggacaatcg ggcgacttcc tctgcccagc
     3601 ggcgagcatt atcaaaatcc gaaagttgga tcgcgtaacc taccagcgtt cccaatagac
     3661 gttgatactc ttccgctggc aatttcttgg ccgcagacgc gatgttggct agctcttgtt
     3721 tcgcccgctt atcatcgcca ctgcggacag caactcgtgc ccgcgataat agcacggcaa
     3781 gggtttgtcc cgattgcttt tcaatctgat cgaggagtcg cgaggcttcg gccgcatcac
     3841 cagcttgcag tttcaaggca acaagcgcca agcccaagga atcgtttttc gcatcctggc
     3901 tcaaatggct ttctagaagt tgttctgctt cttgtaagcg cttttcggcg gccagaatct
     3961 cagcacgtaa aatgatcgca tctgtcttcg tggtatcatc tttttccaag gtatccagcc
     4021 actgctccac ttccagccaa ttacgttcct ggggaggttt actgagcgtg ctgagaacga
     4081 gcaagcgcgc aaattcaaga acaccttgag gagttgcctt tcccaattgc acgacgttgc
     4141 ggtattcgtt gacagcctca tcgagcctac ctaactccag gagcgccatc gctcgtccga
     4201 ggcgtgcctg cggccaaagc gggtcctgtg tcatggctct cgagtaagag gccacacttt
     4261 ggtcaggatt acccatctgg gcataacaga gcccgagaag aaaatccact tgcttaagca
     4321 agtctggcga atctgtaacg tcgggccgca ccgactccaa cttacgagct gccaaaaacc
     4381 actgttcttt ggcggtcaga agtcgcccac gcaagaattc aacttgggca cgtggatagg
     4441 aaaccgcctc gagtttctcg atggtctgct ccgcatccgc caggcgaagg gcatcaatct
     4501 gcatgctggc gagcgcccaa tagaggggac ctttatcctt cactttggag gctgcgtctt
     4561 cgagcgtgtt aattgctgtg tctgcattac ctttggcaaa ctgtgtggct gcatagacgg
     4621 aatagggatc gggtgactcc ggggaggcgg cgatcgcttt ttgagcaaac tcctccgctt
     4681 ggtctaactg ccccaaccgc cgggcactgt ctgccgcgac caagagcatc tcaagattcg
     4741 aaggatcttt tttgtgcgcc tcatcagccc atttcaatgc tgcttcatag ttctgggcgt
     4801 tgtaaaaata ggaagccgct accaaatagg cctcagcgga atctccgaca gttaacacca
     4861 ttttggtaat ccattgatca ccttgctcag gctgtttgag gtcgtgccgg taggtgccgg
     4921 ctaactgagc gtatgcaggg acatagtcct ccttcagctc gatgacgcga cggaaagtgg
     4981 ttgtcgcccg ttcgtactga cccagtcccc tttgacagcg tcccaattcc gttagtagtt
     5041 ctggatcatc cggatactct ttgagaaggt actgctcaat atgttcctgg gcgtctcgaa
     5101 accgtcccat cgcagtgaga acctgcacga gttttcgccg ggtttccagc cggccagggt
     5161 cctgccgcaa gaccatctcc aagcgagtga aggccggcca gtaagccttt agatcggcaa
     5221 gtagactccc atactccgat aaggcgtcca ggtcattcgg gaccaaatcg atataccatt
     5281 gataattctt gaccgccaca tcgaatcgtt cttgggtttt ggcctcccga gcacggtcca
     5341 gaaagaccct tgcattgcgc cgggtttgga atccgtggag gagatagcca ctaacgatcg
     5401 caagaacgac tgcacccacc aggatgacgg caagcttttt gttgacggtt cgcgccattc
     5461 agatatcccc cttaaccgtt cgagctatcc atagagcgcc ccactctttg ccaaattcaa
     5521 ccaagacttc tcagggttca gcgtagagac atgacagggc cgaaaaatct tagcctatta
     5581 taactggggc gtgtctctga tcgatcaccc gaaagggtgg cttgatttac cattctgcaa
     5641 ctaggacttg actttgttct aacctacaga tgctgcacgg gcacaaggtg gaaaacgagt
     5701 tcttaaggga ggcctgtcca gcgaaacaga attgccgaac attacattga gggttgatgg
     5761 aacgatcaaa cgggctgtgc caacgaaacc accacttgtc attacaacac ttatccttaa
     5821 gaattgggtt ggtcgagttg aatcacgctg tccccttgct taaaaggttt tttatgtcac
     5881 tcttccacct agtcaagcgc ttgttgtggt gcccgccgag gcactcatcc ccctcacagt
     5941 taagcggagc cgccatcctc tgtataacgt tgattatttg ttacaccccg ctgacacttc
     6001 cagttctctt gggcgaggag ccgagtgcct ctgttggcgg ctcggcttct ggtagccaat
     6061 cgaaagacgt ggcttccggc gaggtagctg cgaatcaggg caaaggctta actcgcgagc
     6121 tgtgtgccca atatctctcg gaagggacat ttgaacaaaa gattgaagcc attacgtcct
     6181 tggttcaatt gcgagagtcg gggatcgacc tcattcttca agcccttcaa gatcatgatc
     6241 cgcgggttcg tgcaaaggcg atcacggccc ttgcagaatt ggcgccgctt gatgcagaac
     6301 gcgtcatacc tcaactgctg gtgtcgcttg aacaagatga tggatttgtc gatggtcagc
     6361 ctaactggat cagggcctcc caggccattg gaaaatacgg tgacaaggtc ctgcccattt
     6421 tggaaaagct ttggcaggat accgatctta agaagcgcat gatcttctgt atcgtttgct
     6481 acgaattggg accgattgc
//
LOCUS       DAOSUT010000203         6428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5711_length_6428_cov_10.049427, whole genome shotgun sequence.
ACCESSION   DAOSUT010000203 DAOSUT010000000
VERSION     DAOSUT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_6428_cov_10.049427"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1218
                     /gene="queG"
                     /locus_tag="PLD05_14085"
     CDS             91..1218
                     /gene="queG"
                     /locus_tag="PLD05_14085"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOP78618.1"
                     /translation="MPLNIVIRLKDLAKDLGFCCVGICPAVEPPHYGAFLKWLEAGYH
                     GEMHYLAQRKEAYRSPSSILPGARSLIMLGFPYQRDAEQNPPAGYGLIARYANGPDYH
                     KTIRQWLRKLGRFLQAEYPSAAVRGVVDTAPLLERDFAQLAGLGWIGKNTSLIGPDCG
                     SFIFLAALLTTVELEPDPPFAGDRCGSCSACLRACPTGALVSPRVLDARRCLSYLTIE
                     LRGFIPLELRELVGAWFFGCDLCQEVCPWNRRLRPTGSATSEVTNTRVINLIALFRLK
                     EDEFRKMFGPTPLWRAKRRGLLRNAAIVLGNQRSQAAWDVLVSGLEDSEPVVREACAW
                     ALARIDIAAAGPIIRAHLAKETDPAVAASLSGYLAETSSSG"
     gene            1361..2512
                     /locus_tag="PLD05_14090"
     CDS             1361..2512
                     /locus_tag="PLD05_14090"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOP78619.1"
                     /translation="MDSPLVIPLRQAEEPRDLVHRCVQALAEGRLVALPTETIYGVAA
                     SVLVPEAVERLAALKGRKAGHPFTLAIKSGEELWDYVPNLNRVGWRLAQRAWPGPMTL
                     VLKAEGGRSVVERFAPCVRELVSPEGSIGFRVPKHPFVAEVLRLLPAPLVLTSVNRSG
                     EAPATTAEEVVKRLPGLDIVFDDGPSQLGVASTVVRVTEDGWEILRQGSLSEEYVRRM
                     AAAVILFVCTGNTCRSPMAEAICRHMLAEKLGCSSEDLEWRGVVVSSAGLEAYCGDVP
                     SPHAVQIMHERGFDISNHVSRPLTERFVREADCVFAMTKAIRNAIVAQWPEAADRVFL
                     LHPEGKDICDPFGATVETYRMSAGMIEEALLRRCDELLALIGSGKAPHT"
     gene            2605..3114
                     /gene="rpiB"
                     /locus_tag="PLD05_14095"
     CDS             2605..3114
                     /gene="rpiB"
                     /locus_tag="PLD05_14095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOP78620.1"
                     /translation="MRIAIGSDHRGYSIRPKIVAFLKNMGHEVIDVGTFSQSPVDYPD
                     VAEAVARKVSSGEVDRGILFCGTGLGMCIAANKIQGIRAVPCHDDLTAEMSRRHNDAN
                     ILCLSADLLGERLITRIVEVWLNTPFEGGRHARRVEKIAAIEKELKEKCCCPDPQQSF
                     SEENDTKAQ"
     gene            3492..4061
                     /locus_tag="PLD05_14100"
     CDS             3492..4061
                     /locus_tag="PLD05_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78621.1"
                     /translation="MAKKKHRDNFEIDLLLRNVELRTELEPYYDDSLTRVNIHQWPLA
                     AENEYLASMLAWEMAPVLPIYRWFDPELRLPRPETLSDEQLHAILWDTIHKLYQKRIV
                     LEFTDHLSDRELYCLIYRDILPSREKKVDLGNNYLRFDCAFVEGDPEVWLRYYATDAE
                     REAWATTYQQPLPPKEKPPYPRVLPKAPA"
     gene            complement(4085..5962)
                     /gene="glmS"
                     /locus_tag="PLD05_14105"
     CDS             complement(4085..5962)
                     /gene="glmS"
                     /locus_tag="PLD05_14105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOP78622.1"
                     /translation="MCGIVGCVGYSNALEFLLAGLRRLEYRGYDSAGIALAHMERAEA
                     IHVRKTAGRIDRLVSLIEAHPISGNIGIAHTRWATHGGATDRNAHPHLGGDGEVAVVH
                     NGVIENCDSLRTRLEGLGYTFRSETDTEVIAHLIAQLLREAHLDPQEVVAKGYTPLVQ
                     VIQSALSQLRGTWGLAILFRDYPGIIVGARLGSPLIVGTGNGANFLASDASALVGVAD
                     NVVFLSDNEIALITGEWVRVYHRDQGRVQPDFRRLDMSASDVDTGGFAHFMLKEIYEQ
                     PEALKNAMRGRIDLDVASAKFGGLNLSPQQLRQVERIVLTGCGTSWHAGLVGEYQIEA
                     IARIPVEVEYASELRYRNPPLHRNTILFAITQSGETADTLAALRELKRKGYPTLAICN
                     VVGSTIAQEADGGIYLHAGPEIGVASTKAFTSQCLVLALLALYFGRLRDLSFEAGTRI
                     IEKLRELPNLVARALECDEKVRRIADRFYQCRNFLYLGRQFNFPTALEGALKLKEISY
                     IHAEGYPAAEMKHGPIALVDEQTPSVFLLPQGHVYEKVLANMQEIKARGGPIIAVVEE
                     GDQEAWRIADEIIEVPAVDDFLQPIVFIIPLQLLAYHIAVARGCDVDKPRNLAKSVTV
                     E"
BASE COUNT         1356 a         1741 c         1883 g         1448 t
ORIGIN      
        1 ccctctcccg gagggagagg gggacttttt gggataggct ctaaactgac ccggttcaag
       61 cgagccgtcc aaacgtaacg ttatttccgc atgccactca acattgtaat tcgtttgaaa
      121 gaccttgcaa aagacctggg cttctgttgt gttggcattt gcccggcggt ggagccgccc
      181 cattatgggg cctttctcaa gtggcttgag gcgggttacc acggggagat gcactatttg
      241 gcccagagaa aggaggcgta tcgaagcccg tcgtccatcc tacccggcgc acgaagcctg
      301 atcatgctgg gcttcccgta tcaaagagat gcagagcaga acccacctgc tggttatggg
      361 ctcatcgcac gctacgccaa tggccccgat taccacaaga ccattcggca gtggcttcga
      421 aagctgggac gattccttca ggcagaatat ccctcagcgg cggtccgcgg ggttgtcgat
      481 acagcccctt tgttggaacg cgactttgca caattggctg gcttgggctg gattggcaag
      541 aacacctcgc tcattggccc tgattgcggc agtttcatct ttttagccgc actgttgacc
      601 acagtggaac tcgagcctga cccgcccttt gccggcgacc gatgcgggag ttgctcggct
      661 tgcttgcgcg cgtgtcccac cggggccttg gtaagcccac gcgtcctcga tgcccgacgt
      721 tgtctcagtt acctgacgat tgagttgcga ggttttattc ccctggaact gcgggaactt
      781 gtgggtgcct ggttttttgg gtgcgaccta tgccaggagg tatgtccctg gaatcggaga
      841 ttgcgtccta cgggaagtgc cacttcggag gtgaccaata ctcgcgtcat caatttgatt
      901 gcgctttttc ggttgaagga ggatgagttt cgcaagatgt ttggcccaac gcccctgtgg
      961 agggcaaaaa ggcgaggcct gttgcgcaac gcggccatcg tgttagggaa tcaacgtagc
     1021 caagcagcct gggacgtctt ggtgagcgga cttgaagatt ccgagcccgt tgtccgggag
     1081 gcctgtgcct gggctttggc gcgaatcgat atcgccgccg ctggcccaat cattcgcgca
     1141 cacctggcca aggaaactga cccggccgtt gcggcgtctc tttcaggtta cttggcggag
     1201 acttcttcgt ctggataagg tttggcatgg ggtgctgtag gccaaatggg ttgaccaaag
     1261 ttggtggtca ctcctataac tggtcgaagg ggaaggcaga gtatgaacaa cgacacaatc
     1321 ccatgcgaac ccgcttagga gactgctcgt cagcccgatc atggattcac cgttagtcat
     1381 ccccctgagg caggccgagg aaccgcgcga tctcgttcat cgctgtgtgc aggctttggc
     1441 ggaaggacgc ctcgtggcct tgccgacgga gaccatctat ggtgtagcgg catccgtgtt
     1501 agtcccggag gccgtggagc gtctcgccgc cctgaagggc cgcaaggccg gtcatccgtt
     1561 caccctggcg attaaaagtg gcgaggaact ctgggactac gttcccaacc tgaaccgcgt
     1621 tggctggagg ttggcgcagc gggcgtggcc ggggccgatg accctagtcc tcaaggctga
     1681 ggggggaagg agcgtcgtgg agaggtttgc cccgtgtgtt cgggaattgg tatctccgga
     1741 agggagtatc ggtttccgcg tgccaaagca cccttttgtg gctgaggtgt tgcggttgtt
     1801 gccagcgccg cttgtcttga cgagcgttaa ccgatcgggt gaggcccctg cgacgacggc
     1861 tgaggaggta gtcaagcgac tgccgggttt ggatattgtg tttgatgacg gaccttccca
     1921 gctcggtgtg gcatcgaccg tggttcgggt gaccgaggac ggctgggaaa tcttgcgtca
     1981 gggaagcttg agtgaggaat acgtgcgacg catggccgcg gccgtgatcc tgttcgtctg
     2041 cacgggtaac acgtgtcgga gtcccatggc ggaggccatt tgtcggcaca tgttagcgga
     2101 gaaacttggt tgttcgagcg aggaccttga atggcgcggc gtggttgtca gttcggccgg
     2161 ccttgaggcc tactgtggtg acgtgccgag tccccacgcc gttcagatca tgcacgaacg
     2221 gggatttgat attagcaacc acgtgtcacg gcctttgacg gagcggttcg ttcgggaggc
     2281 cgattgcgtg tttgccatga caaaggctat ccggaacgcc atcgtggcac aatggccgga
     2341 ggcggccgat cgggtcttcc tgttgcatcc cgagggaaag gacatttgcg atccctttgg
     2401 ggccacggtc gaaacctatc ggatgtccgc tggtatgata gaggaagccc tgttgcgacg
     2461 atgtgacgaa ctgttggcct taattggcag tggcaaggca ccgcacacgt gactaattgt
     2521 aatggcttgg ttgcacagcc aagggaattt aggaaaacaa acaacaaatt tggatcacag
     2581 tcttaacgtt tagaggtttt tgcaatgcgc atcgctattg gcagtgatca tcgtggttac
     2641 agtattcgtc ccaagattgt ggccttcctc aagaatatgg ggcacgaggt catcgatgtt
     2701 ggcacattca gtcagtctcc cgtggattat cccgatgtgg cggaagccgt ggcacggaag
     2761 gttagctccg gcgaagtgga ccgcggcatt cttttttgtg gaacgggctt ggggatgtgc
     2821 atcgcggcca acaagatcca gggaattcgt gcggttcctt gccatgatga tttgacggct
     2881 gaaatgagcc gccgtcacaa cgatgccaat attctctgct tgtcggcaga tttgctgggc
     2941 gaacggctga tcacccggat tgtcgaggtc tggctcaata cacctttcga aggtgggcgg
     3001 cacgcgcgtc gtgtcgagaa gatcgcagcc attgaaaagg agttaaaaga gaagtgttgc
     3061 tgtcctgatc cccagcaatc attttcggaa gagaatgata cgaaggccca atagaccgcg
     3121 gcaggctgat gggtgatcgt gtatcgcatc gtggcaaaac aagaaaagac ggctgcttgc
     3181 aaaacttgag gaaatattgt cgtcttctct gattgttcgg cccgaggaac atcagatttt
     3241 tcggctgtag ggcttttgcc accgccgcga ttctctttga agatttttgg ggcttctgac
     3301 gacttgaagc cgcctcatgc gtgagtacag tcgaatatgg ggaccgatga atgcggcgtg
     3361 atgtcgcgtg gtcgtgtctc atcgggtagt tgtggttcct gatagtgggg acacgtgtcg
     3421 tctcaacttg tttcaaacgt ttccaactag gcagcaggga tttgcagcgg gagattgagg
     3481 aacgatcggc aatggccaag aaaaaacatc gagacaactt tgagattgac cttctgcttc
     3541 gcaatgtcga gttgcggacc gagctggagc cttattacga cgattctttg acgcgggtca
     3601 acatccatca gtggccattg gcggcggaaa atgaatacct tgccagcatg ctggcgtggg
     3661 agatggcccc cgttctcccc atttatcggt ggtttgatcc ggaattacga cttccccgcc
     3721 ctgaaactct cagcgacgag caattgcacg ccatcctttg ggatacgatt cacaagctct
     3781 atcagaagcg gattgtgctg gagtttactg atcacctctc ggaccgcgaa ctgtactgcc
     3841 tgatctaccg cgatatcttg ccgtcgcgag agaaaaaggt cgatttgggt aacaactatc
     3901 tccgctttga ttgcgcgttt gtggaaggtg atccggaggt gtggctgcgg tattacgcca
     3961 ccgacgcgga acgggaggcc tgggcgacca cctatcagca gcctcttccg ccgaaagaaa
     4021 agccgcccta tccgcgggtt ttacccaagg ctccggcgta actgcccaaa tgtgaacgac
     4081 gagcctattc caccgtgacg ctcttggcaa gattgcgcgg cttgtccacg tcgcaccctc
     4141 gtgccaccgc aatgtgatag gccaagagtt gaagcgggat gataaagacg atgggctgaa
     4201 gaaaatcatc gacggcggga acctcgatga tctcatcagc tattcgccag gcctcttggt
     4261 caccctcttc cacgacggcg atgatcggtc caccgcgggc cttaatctcc tgcatattgg
     4321 ccagcacctt ttcatagaca tgcccctggg ggagaagaaa gacactgggc gtctgttcat
     4381 ccaccaaggc aatcggccca tgcttcattt ccgccgcggg atacccctca gcatgaatgt
     4441 agctgatctc cttgagcttc agtgcaccct ccagggccgt gggaaaattg aactggcgcc
     4501 ctagatacag gaaattccga cactggtaaa agcggtcggc aattcgtcgc accttttcgt
     4561 cacattccag ggcgcgggcc acaagattgg gaagttcccg cagcttctcg atgatacggg
     4621 tgcccgcctc aaagctaagg tccctcaaac gtccaaaata aagcgccaac aaggccaaga
     4681 cgaggcattg cgatgtaaag gccttcgttg acgccacacc gatttcggga cccgcgtgca
     4741 gataaattcc gccatcggcc tcctgagcga tggtgctgcc gaccacgttg catatcgcca
     4801 gtgtgggata ccctttccgt ttcaactctc gaagggcggc cagagtgtct gctgtctcac
     4861 cgctttgggt aatggcaaaa agaatcgtgt ttcgatggag cggaggattg cgataacgga
     4921 gttcgctggc atattcgact tccacgggaa ttcgggcaat tgcttcgatc tgatattcgc
     4981 ccaccaagcc ggcgtgccaa ctcgttccgc aacccgtgag cacaatcctc tccacctggc
     5041 gaagctgttg cggcgacaga ttaagaccgc cgaacttggc ggaggccacg tcgaggtcaa
     5101 ttcgcccccg catggcattt ttgagggctt ctggctgttc gtagatttcc ttgagcataa
     5161 aatgggcaaa gccccccgta tcgacgtcgc tcgccgacat atcgagtctg cggaagtcgg
     5221 gctggactcg cccctgatct cggtgataga cccgcaccca ttcgcccgtg atcaaggcaa
     5281 tctcgttgtc actgaggaaa acgacgttgt ccgcaacccc gaccaacgcc gaggcatcgc
     5341 tggccaggaa attcgcacca tttcccgtcc cgacgatcaa aggactgccc agtcgggcac
     5401 cgacgataat gccgggataa tccctgaaca gaatggccaa cccccaggtc ccccgcagtt
     5461 gggacaaggc cgattgaatg acttgaacga gcggcgtgta ccccttggcg acgacctcct
     5521 gagggtccag atgtgcctcc cgcaagagtt gggcgatcag atgtgcgata acttccgtgt
     5581 ccgtttccga tcggaaggtg tagcccaagc cctccaaacg ggttcgcaaa gaatcgcaat
     5641 tctcaatgac gccgttatgg actacggcca cttcgccgtc gccccccaag tgcggatgcg
     5701 cattgcgatc ggtggcccca ccgtgtgttg cccagcgggt atgggcaatt ccgatatttc
     5761 cggaaatggg atgcgcttcg atgagcgaca ccaggcgatc aatccgtccg gcggtcttgc
     5821 ggacgtgaat tgcctcagcg cgctccatat gagccaaagc gataccggcc gagtcgtatc
     5881 cacggtattc cagccgtctc agccctgcca aaagaaactc aagagcattc gagtagccaa
     5941 cgcagcccac gattccacac atggatctcg atactcctta cgcgacattt gtgacacgag
     6001 caaacggcta agagcctctc ccataatctc ctctcacccg ttcggcggga gagggtccgg
     6061 gggcgagggt ggctctggtt gctaccggtt ttctcaatcg actccggtga tcgcttgccc
     6121 tcaccccaac cctctcccgg agggagaggg agctttttgg gatagactct aagccgagaa
     6181 taacactttt cgccaaaacg ttcacggttg attttgagga gtttccggac ttttcccggc
     6241 tgatatgaca agataggcga tctggagaaa tctcccccgg cgatcacgga cgctcgcgca
     6301 aacggtgtgc catctatacc actttagtcg gacttgacct cgggcgtcaa actaaaacca
     6361 gcaaactacg atgacgtccg gcgaagagca cacaagtggc ccattgtctc ttaagaacct
     6421 gtcccata
//
LOCUS       DAOSUT010000204         6332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5778_length_6332_cov_10.165525, whole genome shotgun sequence.
ACCESSION   DAOSUT010000204 DAOSUT010000000
VERSION     DAOSUT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_6332_cov_10.165525"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLD05_14110"
     CDS             complement(<1..932)
                     /locus_tag="PLD05_14110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOP78623.1"
                     /translation="MGVLLSILFAALVAGLFVWFFHWLERYRREWIYLFGCVFGWGVV
                     SGAFATLFLALAVPWGMAFHLESEAFEVPRRFLFLAPAVEEGLKALAILAVMLMFRRE
                     FSYLPEGMVYGGVIAAGFAASQNVFYFYHAGYLEGGLVGLLAIFVQRGLLGGFTAVLC
                     GAGIGAGLAWAQRRPKRWQRWHTAITLWTGVYLCHILRNGLVLVIGREFGWKPLTAGT
                     LVDLAAWLVAGALVLGLLRGEYRRFLDCLRREVEEGWLKPEQAELLKRQFWKARLRMI
                     FPLWQDRTCRVIFLFSELAQQQVVAAETARGVGG"
     gene            complement(1080..2258)
                     /locus_tag="PLD05_14115"
     CDS             complement(1080..2258)
                     /locus_tag="PLD05_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78624.1"
                     /translation="MTEEPLSQSEKNHWDKLAELLGIKPSAEPQPSQEDVARGEQAAQ
                     LETAQTPQECSQDIASQRGDQIAPEKTPVVPDSIPLRRTWVTKPRSAGNWDEIIGQLG
                     LSTSAASPTPVSASVEPSGVSADAVSVPPQATGEKVSPELTAEGEARPVLTPLEQTIV
                     PPALEEASVVALATPEEKPPIVQEEVTPPGEWLEEQVVAPVEVIEEAEEVWPQTHDIL
                     DIKDSRDAVAADTLLVTEVPQVLQDKELGEPEGKRGEKKGRKRHRRKKKSRAPVTPEP
                     FEEELPEIVELATTGEDNGTTAESSPSQGMTVGAGRERTASAKADRKKRPRPSHDVAE
                     DAAEAEIDAGEADEDDEAAEISRPSHKAIPGWAEVVGYVIEKNLEARSRRPNHRDRRR
                     "
     gene            2862..3497
                     /locus_tag="PLD05_14120"
     CDS             2862..3497
                     /locus_tag="PLD05_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOP78625.1"
                     /translation="MFLDQWIALQGTAQASYRDYQRQRQMTLGDLLLENRIILLQGVI
                     HDGNASEVIMKLLYLQSENRRKDIHFYINSPGGSVTATLAIYDTMQILSCPVATYCVG
                     LAASGAAVLLAGGTKGKRFALPHSKVMIHQPYGQVGGQVTDIEIQTQEILRTRDILNR
                     ILSEHTGQPIERIAQDTDRDFYMTAEEAKNYGLVDDILTKQSQEKAEEEIE"
     gene            3636..4238
                     /gene="clpP"
                     /locus_tag="PLD05_14125"
     CDS             3636..4238
                     /gene="clpP"
                     /locus_tag="PLD05_14125"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOP78626.1"
                     /translation="MTLIPFVIERTGREERAMDIYSRLLKDRIVFIGAPITDELANSV
                     VAQLLFLQSEDPKADVHVYINSPGGSVTAGLAIYDTMQFIRCDVATYCIGQAASMAAV
                     LLAAGTKGKRYALPNSRIMIHQPMAGVEGSATEIMIHAKEFKNLKQRINQILIKHTGH
                     PLEKIEKDTDRDCFMSAEEAKDYGLIDHVIERAPEPAPQG"
     gene            complement(4438..5379)
                     /locus_tag="PLD05_14130"
     CDS             complement(4438..5379)
                     /locus_tag="PLD05_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOP78627.1"
                     /translation="MDQDYSELLQRLRTNINGVLLGKESVVEMCLVALLAGEHILLED
                     VPGVGKTLLGKALAKSIAGEFRRIQFTPDLLPSDITGSNLYNAERREFVFYPGPIFAN
                     IVLADEINRATPRTQSALLEAMNESQVTAEGTTFPLPQPFLVIATQNPAEFEGTYPLP
                     ESQMDRFLLRVSIGYPSREYERQLLAQHRDGEPVDRLEPVLSCEQVLALQAATRQVYV
                     EDSVADYLLNLVHATRECPEIRVGASVRASLALYRAAQAKALLDGRDYVVPDDVQSLA
                     VPVLAHRVITHGYLHGEHREAVEALIHRLVEDTPSPT"
     gene            5671..>6332
                     /locus_tag="PLD05_14135"
     CDS             5671..>6332
                     /locus_tag="PLD05_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOP78628.1"
                     /translation="MSVWKTIRGRFIGQPLGKKEETQQPTDPRELEAERCIHQGLELL
                     AAGQIDEALAAFHRAVQAAPHSARAHYGRGLAYYNRGDYHWAIADLSQALRLNPHMAE
                     AYCTRGLACAAKGDYDWAIADLTEALKLNPYLAEAYNGRGVAYTAKKCYDAAIADFDC
                     AIKLNPNDANVYFNRGFAYGLKGDYPRALADYTQAIRLNPQHYRAYNNRGTIYQALHD
                     YQR"
BASE COUNT         1377 a         1921 c         1763 g         1271 t
ORIGIN      
        1 tggccgccga ccccgcgggc cgtctccgcc gcaacgactt gctgctgggc cagttcgcta
       61 aacaggaaga tgacgcgaca ggtgcggtcc tgccaaagcg ggaagatcat ccgaaggcgt
      121 gccttccaga actgtcgctt gagcaattcg gcttgttccg gtttgagcca gccctcttca
      181 acttcccgcc gcaggcagtc gaggaatcgt cggtactccc cgcggaggag ccccaggacc
      241 aaggccccgg ccacgagcca ggccgcgaga tcgacgagcg tgcccgccgt gagcggcttc
      301 caaccgaact cccgcccgat gaccaggaca agtccattgc ggaggatgtg gcaaagatag
      361 acgcccgtcc acaaggtgat cgccgtatgc catcgctgcc agcgcttggg gcgtcgttgg
      421 gcccaggcaa gccccgcacc gatccccgca ccacatagca cggccgtgaa cccgcccagc
      481 agtccccgct ggacaaatat ggccagcagg cccacaaggc caccttccag atagccggcg
      541 tgataaaaat agaagacgtt ttggcttgct gcgaaccccg ctgcgatgac accgccatag
      601 accattccct cgggaaggta cgagaactct cggcggaaca tgagcataac ggccaaaatg
      661 gccagggcct ttaggccttc ttcgacggca ggtgcaagaa acaagaaacg acgtgggacc
      721 tcaaaggcct cgctttccag gtgaaaggcc attccccagg gcacggccaa ggccagaaat
      781 agcgtggcaa aagcgccgct gacgactccc cagccaaaga cgcagccgaa caggtaaatc
      841 cattcgcgac gatagcgctc gagccagtga aagaaccaaa cgaataggcc cgccaccaaa
      901 gccgcgaaca aaatcgacaa cagcacgccc atgagtttcg tcttccttaa aatttgacgt
      961 gacttgctgc ttcaccactc cgtcggtctg actaacgtgc tgaatccctc tcccattccc
     1021 cacacgaaaa ccatgctcag ccctacctac gtctcttgcc cgatgccccc atcccacact
     1081 taccgccgac gatcccgatg gtttggccgc cgggagcggg cctcgaggtt cttttcaatg
     1141 acgtaaccca ccacctctgc ccagcccgga atggccttgt gcgagggccg cgaaatctcg
     1201 gccgcttcgt cgtcttcatc ggcctccccg gcgtcgattt cagcctccgc cgcgtcctcg
     1261 gccacatcat gcgacgggcg cggccgcttt ttgcgatccg ccttcgcact tgccgttcgc
     1321 tctcgccccg ccccgaccgt cattccctgc gaaggtgaag attcggccgt tgtcccatta
     1381 tcttcccccg tagtcgccag ctcgacgatc tccggaagtt cttcctcaaa tggctccggg
     1441 gtgaccggcg cgcggctctt ctttttgcgg cgatgacgct tgcggccttt cttctctccg
     1501 cgtttacctt caggttcccc taactctttg tcttgcaaaa cctgcggcac ctccgtgacc
     1561 agcaaggtat cggcagccac cgcgtcccgg gaatccttga tgtcgaggat atcgtgggtc
     1621 tggggccaaa cctcttccgc ctcctcgatc acctcgacgg gggcaacgac ctgctcctca
     1681 agccattcac caggcggtgt tacttcctct tggacaatgg gaggcttctc ttccggtgtg
     1741 gccagggcca cgacggaggc ctcctccaag gccgggggaa cgatcgtctg ttccagcggc
     1801 gttaacaccg gccgcgcctc accctcggca gttaattcgg gcgaaacctt ttctcccgtg
     1861 gcttgaggag gtaccgaaac cgcgtccgca gaaacgccgc ttggttccac cgatgcacta
     1921 accggtgttg gggaggcagc agacgttgac aagcctaact gtccgatgat ctcgtcccag
     1981 ttgcccgcac tccgtggctt tgtcacccaa gtgcgtcgca gggggatgct gtccgggaca
     2041 accggtgtct tttccggtgc aatttgatca cccctttggg atgcaatgtc ctgggagcat
     2101 tcttgaggtg tttgggcggt ctcaagctgg gcagcctgtt cccctcgggc aacatcctcc
     2161 tgggacggct gtggttccgc tgacggtttg atccccaaca actccgccag cttatcccag
     2221 tggttctttt ctgattgaga caatggttct tccgtcatat gaagataacc aggcttggga
     2281 cgcctaacat gcaaaagaca gtttcaccgt ttggtcaaag gtcaataggc ctgtgacact
     2341 tttcaaggag cgaaactcgg ctcaagggcc accggtctcc cgcggcggtc cagcaaaggg
     2401 gccgaagacg cgaagatcgg tggactttca caagccgcta atgcccaccc gcaagctgta
     2461 tcgccggcgc aacgcataac cctccttgat gacaattaat ataagggatt ggaagccccg
     2521 cgaaaagcat ggccggcgcg tcagccccgg taggtcggtg gtgggagaca aaaatcatcc
     2581 tggaaagcca cgcgcagtgg cggccacgga ggaccctaga ccatgaatgg gcctgacggc
     2641 gccctcgatg gcgtgtccct ccccgggtac cccgacttgg gcacacacag cttgccagac
     2701 caataggcca accggtacga taacattttt ccggcgatcc gccgtactca agtcacaact
     2761 agacgtccgg ccgggtaatt gtgcctcaac ggatgcaccc aacggacgct cggatcgtcc
     2821 ctatcgatag atggaaattt ctgaaaaaga aggacgtgat tatgttcctg gatcaatgga
     2881 tcgctctgca agggaccgca caagcaagtt accgtgatta ccagcggcaa cgtcaaatga
     2941 cgctgggaga cctccttctg gagaatcgca ttattctcct ccagggggtt atccacgacg
     3001 gaaatgctag tgaagtgatc atgaaacttt tgtatttgca aagtgaaaat cggcggaaag
     3061 acatccattt ttacatcaat tcgcctgggg gaagtgttac cgctacgctg gcgatctatg
     3121 acacgatgca gattttgagc tgcccggtgg caacttactg cgtgggctta gcggccagcg
     3181 gtgcagccgt gctccttgcc ggtggcacga agggaaaacg cttcgccctt ccgcattcca
     3241 aagtgatgat ccatcagcct tacggccagg tcggtgggca ggtgaccgac atcgagatcc
     3301 agactcagga aatcctccgc acgcgggata ttctcaaccg tatcctttcc gaacacacag
     3361 ggcagcccat cgaacggatt gcccaggaca ccgaccgcga tttctacatg acggccgagg
     3421 aggccaagaa ttatggtctt gtggatgaca tcctgaccaa gcaatcccag gagaaagcag
     3481 aagaagagat tgagtgagtc cttgagggag tccctccgtg gggatgggaa cgtccttgag
     3541 gctcgttgat cgagcgatga gacctagccg aagaccggcc ccgaaagaga ccattgatcc
     3601 taaggaaaca atgtcagtga ttgaggagac atatcatgac actcataccg tttgtcattg
     3661 aacgtacggg ccgagaagag cgggcgatgg atatttacag tcgcctgctc aaagaccgta
     3721 tcgtgttcat tggggcaccc atcacagacg agttggccaa ttcagtggtg gcgcaactcc
     3781 tcttccttca atcggaagac ccgaaagcgg atgtgcatgt ttatatcaac tcaccgggcg
     3841 gaagcgtcac ggcaggcttg gcgatttacg acacgatgca attcattcgc tgcgatgtag
     3901 ccacctactg cattgggcaa gcggcttcca tggcggcggt cttactggcg gcgggcacca
     3961 agggcaaacg ctacgccctg cccaattcac ggatcatgat ccaccagccc atggcaggtg
     4021 tggagggaag cgccacagag atcatgatcc acgccaagga gttcaagaat cttaaacagc
     4081 ggatcaatca gattcttatc aaacacactg ggcatcctct ggagaagatt gaaaaggata
     4141 ccgaccgcga ttgcttcatg tcggccgaag aagccaagga ttatggcctg atcgaccatg
     4201 tcatcgaacg agcaccggag ccggcacctc aaggatgatc cgcccgaggc atgaacttct
     4261 aggatggcag cagcggctta gcgcctatcc cacaaggtcc ccctctccct ccgggagagg
     4321 gttggggtga gggcaagcga acattgcaat cgactgagga aaccggcagc gattcgagcc
     4381 accctcaccc ccgacccctc tcccaccgaa cgggcgagga agattgggga tagcctttta
     4441 ggttggcgac ggcgtatcct ccaccaggcg gtggataagg gcctcgacgg cctcccgatg
     4501 ttctccgtgc aaatagccat gggtgatcac gcggtgggca agcaccggaa cggccaggct
     4561 ttggacgtcg tccggcacta cgtagtctcg accatccaag agggccttgg cctgggccgc
     4621 ccggtacagg gcgagtgatg cccgcacact ggcccccacg cgaatctccg gacattcccg
     4681 tgtggcgtgg accaagttga gaaggtagtc cgccacactg tcttccacat acacttgccg
     4741 cgtagctgct tgaagggcga ggacttgctc gcaggaaagg accggttcca gtcgatccac
     4801 gggttcgcca tcccggtgct gcgccagcag ttgccgctcg tactcgcgac tagggtaacc
     4861 tattgaaacg cggagcaaga agcgatccat ctggctttcc ggcaggggat acgtcccctc
     4921 gaattcggcg ggattctggg tggcaatgac cagaaacggc tgcggcaggg gaaacgtcgt
     4981 cccttccgcc gtcacttgcg attcattcat ggcctcgaga agggcgcttt gcgtgcgcgg
     5041 cgtggcacga ttgatctcat ccgccaagac aatattggcg aaaataggac cgggataaaa
     5101 gacaaattct cgtcgctccg cgttatataa gttgctcccc gtaatgtctg aggggagaag
     5161 gtcgggcgtg aattggatgc ggcggaactc cccggcgatg ctcttggcaa gggcctttcc
     5221 caaaagggtt ttgcccacac cggggacatc ctcaagcaag atgtgttccc cggcaagaag
     5281 ggccaccaga cacatttcga ccacagattc cttcccgagg aggaccccgt tgatattggt
     5341 ccgcagtcgc tgcaaaagct cggaatagtc ctggtccatc gaatgcgccc tcgccctcag
     5401 ccgtctttgg taaccccgct cggaggatcg tgtcaccgga agatcctcag tgtttcgggt
     5461 atcttgccaa ctccactcag tgtacccatt tcgacaggta actgtcatgg taggtcattc
     5521 tttgtggggg gatacgtgtg cctggctcca tcttgccgcg ggcgccgctt gcctcacgtg
     5581 gcatgcaact tgccaattgc gagtaccgtt cgtatctttc agaggggtct tccggtagcc
     5641 ggttcatcgt gctgttaggg ggaaacggcc atgtcggtgt ggaaaaccat tcgcgggcgg
     5701 tttatcgggc aacctctcgg aaagaaagag gaaacgcaac agccgacaga cccccgagag
     5761 ctcgaggcgg aacgctgcat ccaccagggg ctcgaactcc ttgctgccgg ccaaattgat
     5821 gaagctcttg cagccttcca tcgtgctgtc caggcagcac cgcattcggc ccgggcccat
     5881 tatggtcggg ggcttgccta ttacaatcgt ggcgattacc actgggccat tgccgatttg
     5941 agccaggccc ttcgtttgaa tccccacatg gcagaggctt actgcacccg ggggctcgcc
     6001 tgcgccgcaa aaggcgacta tgattgggcg attgccgatt tgacagaggc cttgaaactc
     6061 aatccctacc tggcagaggc ctacaacggc cgtggtgtcg cttacaccgc caagaagtgc
     6121 tacgacgctg ccatcgctga ctttgattgc gcgattaagc ttaatcccaa cgatgccaac
     6181 gtctatttca accggggctt tgcctatggg ttgaagggtg attaccccag ggccctagcc
     6241 gactataccc aagccatccg tcttaatccc cagcactatc gcgcgtacaa caatcgcggg
     6301 accatctatc aggctttgca cgattaccaa cg
//
LOCUS       DAOSUT010000205         6290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5824_length_6290_cov_9.456455, whole genome shotgun sequence.
ACCESSION   DAOSUT010000205 DAOSUT010000000
VERSION     DAOSUT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_6290_cov_9.456455"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLD05_14140"
     CDS             <1..351
                     /locus_tag="PLD05_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78629.1"
                     /translation="YVLNGGEVAAMVMIDAVIRLIPGVLGDEESSCLDSFSGPERLLD
                     YPQYTRPRCYRGYEVPAVLLSGNHQAVRRWRLQEQRRLTAQRRADLLQHGLSSEQCEH
                     IVETPADPPSASGQ"
     gene            457..804
                     /gene="rplS"
                     /locus_tag="PLD05_14145"
                     /pseudo
     CDS             457..804
                     /gene="rplS"
                     /locus_tag="PLD05_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            1106..1486
                     /locus_tag="PLD05_14150"
     CDS             1106..1486
                     /locus_tag="PLD05_14150"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOP78630.1"
                     /translation="MASADDPRAQLGHAGEQAAMDFLIKRGHTILARNVRYPEGELDI
                     VTRDGQCLVFVEVRTRRSEKHGECWEGISRAKQRRLIRAAHRFRREGRLFSVPCRFDV
                     ITVLWPEGCHEPIVTHFQRAFDAD"
     gene            complement(1543..2370)
                     /gene="folP"
                     /locus_tag="PLD05_14155"
     CDS             complement(1543..2370)
                     /gene="folP"
                     /locus_tag="PLD05_14155"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOP78631.1"
                     /translation="MVDRWTIRNGTLDLTRRPLLMGILNVTPDSFSDGGRYFDHQAAI
                     EHGEQLAAEGADILDIGGESTRPGAEPVPLEEELRRVIPVVEALAPRLSIPISIDTSK
                     AMVARQAIIAGAAIVNDVTALRGDPDMVDVVRETGAGVCLMHMKGTPRTMQQEAVYED
                     VVSEVASFLLSRCRELESAGIPSDRLAIDPGLGFAKTPHQNWQIIQGLQQLRSLGYPL
                     VIGHSRKRFIREVVGNDFRSVLWGTVGVGLLLAAGGVDILRVHDVKAMREALALWRR"
     gene            complement(2377..3927)
                     /locus_tag="PLD05_14160"
     CDS             complement(2377..3927)
                     /locus_tag="PLD05_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOP78632.1"
                     /translation="MAFEELPPLDAMAERLQRDVRRGSSAVFVAQVVSQLISLVVLAI
                     LLRTLGLEPYGLLGMVLPLLALVRIFIYSGLDVAAIQDPLLDRQRASALFWINQILGM
                     LGTAIVAVSAPWISWFYGREELVPLTIALSGTMLVTTLGAQHQALLQRDLQLGRLSII
                     RVAAQVIGGVAALIAAWAGAGIWTLVVQQYAEPAALTVLSWVVTGWRPAFVLRGAGSR
                     ELLHFGGHYTMSAFLLYLMSHVDKIVVGRFLGSQALALYYQAFNLAQKPVGLVVSPLT
                     GVMLPALARARSEASLYRQYATRFLRFLNWIMFPCGIGLALVADETMAVLGGPRWSGA
                     GPILRIFALMIPFQALFNVTGTIYASLGRADRMARACIPVTAVMCVAFLVTVTVARTS
                     PHAVHILAWVYFLTFAVSVLAYTGYAFWMSGLSLRSVLGTLHAIWLGLLLMIVAVWAT
                     RVGFTLLRFQSDYLLLFSEIVAGMIAYFIGTRRISLEYLIGSLKGFWPESQQGLPERE
                     DRAESQEV"
     gene            complement(3947..4813)
                     /locus_tag="PLD05_14165"
     CDS             complement(3947..4813)
                     /locus_tag="PLD05_14165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOP78633.1"
                     /translation="MNSPSLDPERLRRGLVQYLEDMQHAGINHLIWPSAETIDSSLLG
                     SQQVSSDDTRLGQSEPLEAQARQGSEENRVTGQLPVVDTVAPLGEIQPLSESREDVLA
                     ELAKQVAQCTRCPELVANRTQTVFGVGNPYSELVFIGEAPGVDEDKQGEPFVGRAGQL
                     LTAMITNGMGMRREDVYICNILRCRPPNNRTPTREEADRCRPWLDATLAVIQPKYICC
                     LGNVAAQNLLRTDVGIGKLRGKVWEYDRAKVVCTYHPAFLLRNPAMKREAWEDLKLLL
                     QVMGRPVPRRPS"
     gene            complement(4973..5443)
                     /locus_tag="PLD05_14170"
     CDS             complement(4973..5443)
                     /locus_tag="PLD05_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78634.1"
                     /translation="MLMPNEQVVKVVWPSITAWPLGRWVGRVWGWALARGPLAALCAG
                     FFLAPVAALVYLWRLRPRRCRRYLVTSERLLLVEGLIARVVWDAPWSQISRLEVVTHP
                     GQVALQAGDVQVYEGDRMLFTLPGVSLVENFIRICHRTQQAKLQVSQLRQRRRA"
     gene            complement(5541..5702)
                     /locus_tag="PLD05_14175"
     CDS             complement(5541..5702)
                     /locus_tag="PLD05_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78635.1"
                     /translation="MSIGLSSNLGLGVSWFSKRIGLLRQEIRELLGHELAEIDKLVQE
                     MLGLAGAES"
BASE COUNT         1484 a         1843 c         1725 g         1238 t
ORIGIN      
        1 tacgtcctca acgggggcga agtcgccgct atggtgatga ttgatgcggt aatccggttg
       61 attccgggtg tcctgggcga cgaggagagc agttgcttag attcgttttc cggcccggag
      121 cgactgctcg attatcctca atacacgcgg ccaaggtgtt accggggata tgaggtcccg
      181 gccgtgttgt tgagcggaaa tcaccaggct gtccgccgct ggcggttaca ggaacagagg
      241 cggctgacgg cccaacgccg tgccgattta ctgcaacacg gtttgtcaag cgaacagtgc
      301 gaacatattg tggaaacacc ggctgaccca ccatcggcaa gtggacagtg acgtttcggc
      361 accggagcga attcggcagc atcttaaaat tgctcagact gtggtggggt tgaaagagaa
      421 aacgaaaatc cacttataac caattcgagg ctggccatga ataagggtga attgatcaag
      481 gctgttgagc aaacctgctt gaagaccgac atcccctatt ttgaagtcgg tgatacggta
      541 gatgttcatt gccgtatcat cgaaggcgac aacgaacgga tccagatttt caacggcgtc
      601 gtcattgccc gaagtggttc gggaacacgg gaaatgttca ctgttcggcg gatcgtgcag
      661 ggcgagggtg tggagcgaaa attccccctc cactcgccgc ggatcgccaa gatcgaggtg
      721 aagcgagcgg cgatcgtccg tcgagccaag ctgtactttc tccgcgaacg ggtggggaag
      781 gccgtccggt tgcgcgagcg ccgtcctgac cagatgcaga aacgtctcaa tcgcgagaag
      841 gcctattggg ccaagcgggg gggacagcct caagaggcct aagcccttca gcagccacaa
      901 cctggcgatc ggcatcacag tttcgccggc aattgattgc ttttgggttg tctaaaacgt
      961 gaccatcatt aagcggccag ttcgatcgta tgacgattcg ggccggccgt ctttcttttc
     1021 gtctttgtgg cggttatttc gccgtctggt agaattcctt gttcgccggc gatcgcgaac
     1081 tctgcttgcg gctaaccagt gcgcggtggc ttctgcggat gacccgcggg cacagcttgg
     1141 tcatgcaggc gaacaggccg ccatggactt cttgatcaaa cgtggccaca ccatcctcgc
     1201 ccgaaatgtg cgttacccgg agggtgaact agacatcgta acccgtgacg gtcagtgcct
     1261 tgttttcgtc gaggttcgta ctcggcgatc ggaaaaacat ggggaatgct gggagggcat
     1321 tagtcgagcc aagcaacggc gtctcatacg tgcggcgcat cggtttcgtc gggagggccg
     1381 gcttttctcc gttccctgcc gctttgatgt catcacggta ctttggccag agggctgtca
     1441 tgagccaatt gtcacgcact tccaacgggc cttcgacgct gattgattaa accgagaaga
     1501 gttgtctccc aatggtatta ccgtgaggcc tacggcttga tgtcacctcc gccataaggc
     1561 aagggcctct cgcatggcct tcacatcgtg aacacgcaag atgtccactc cccctgccgc
     1621 caggagtaac cctacaccga ccgtgcccca aaggaccgac cggaaatcgt tgcccacaac
     1681 ctcgcggata aatcttttgc gggaatgacc tatgaccagc ggataaccca aactacgcag
     1741 ttgttgcaat ccttgaatga tttgccaatt ctggtgcggc gttttcgcaa atcctaagcc
     1801 ggggtcaatg gccaagcgat ctgagggaat tccggccgat tccaattctc ggcatcgcga
     1861 cagcaggaaa ctggcaacct cgctaacgac gtcctcgtaa acagcttcct gctgcatggt
     1921 tcggggcgta cctttcatgt gcataaggca taccccggca ccggtttccc ggacgacatc
     1981 gaccatgtcg gggtctcctc gaagggccgt gacatcgttg acgatagccg cccccgcgat
     2041 aatggcctgt cgagctacca tcgccttgga ggtatcaatg gagatgggaa tcgaaagacg
     2101 cggagcgagg gcctcaacca ctggaatgac acgcctgagt tcttcctcga gaggcacagg
     2161 ctcggccccg ggccgtgtgc tttcgccgcc aatatccaag atgtccgccc cttccgcggc
     2221 aagttgttcg ccgtgttcga tggccgcctg atggtcaaag taccgcccgc cgtcggagaa
     2281 actgtcaggc gtcacattca ggatccccat aaggagtggc cgtcgtgtga ggtcaagcgt
     2341 cccgttacgg attgtccagc gatcgaccat ggaacgttat acctcctgcg actccgcccg
     2401 gtcttcgcgt tccggcaggc cctgctggga ttctggccag aaccctttca ggctgccgat
     2461 caggtactcc agggaaattc gccgtgtccc gataaagtag gcaatcatgc ctgctacaat
     2521 ttcgctgaaa aggaggagat aatccgattg aaaacgcaac agcgtaaagc ccacccgcgt
     2581 cgcccacaca gctacaatca ttagcagcaa acctagccaa atggcatgca gtgtgccaag
     2641 caccgacctg agagaaagcc cgctcatcca gaaagcgtat cctgtgtaag ccaggaccga
     2701 aaccgcaaac gtcaggaaat agacccaagc caagatatgg acagcatggg gcgaagtccg
     2761 cgccacggtc accgtcacca gaaaggcaac gcacatgacg gccgtgaccg ggatgcacgc
     2821 ccgggccatc cggtcagcac gtcccagtga agcgtagatg gttcccgtaa cattgaaaag
     2881 ggcctggaaa gggatcatga gggcgaagat tctcaggatc ggtcccgccc ctgaccagcg
     2941 aggtccgccg agcacggcca tggtttcgtc cgccaccaga gccaacccaa tcccgcaggg
     3001 aaacatgatc caattcaaga agcgaagaaa ccgagtagcg tactgccggt agagagaggc
     3061 ctccgagcgg gcacgggcca aggccggcag catgacgccc gttagaggag aaaccacaag
     3121 acccaccggt ttttgggcca gattaaaggc ctggtaataa agggccaggg cctgcgaacc
     3181 gaggaaacgt cccacgacaa tcttatcgac atgactcata agataaagga gaaaggcact
     3241 catcgtgtag tgaccgccaa agtgcaatag ttcacggcta ccggccccgc gaagcacaaa
     3301 ggccggacgc catcccgtga ccacccagga aaggaccgtc aaagccgccg gctcggcata
     3361 ttgctggacg acaagggtcc aaattcccgc ccctgcccac gccgcgatca aggcagccac
     3421 acccccgatg acctgcgcgg caacccgaat aatcgacagt cgtcccaatt gcaaatcccg
     3481 ctgtaaaagg gcctggtgtt gagcccccaa cgttgtgacg agcatcgtcc cggaaagggc
     3541 aatcgttaaa gggacgagtt cctcccggcc ataaaaccaa ctaatccacg gcgcactgac
     3601 cgcgacgatg gccgttccta acatgcccag gatttgattg atccaaaata gggcggaggc
     3661 cctttggcga tcgagcaagg gatcctgaat ggctgccaca tcaaggccgg agtagataaa
     3721 gatgcgaacg agcgccagga gaggaagtac catccccaat aggccataag gctccaagcc
     3781 caacgtccgt aacaaaatgg ccaggacaac cagggaaatt aattgagaaa cgacctgggc
     3841 aacaaagacg gctgagctcc cgcgccgaac atctcgctga agccgctcgg ccatcgcatc
     3901 aaggggtggc agctcctcaa atgccatcgt gcaattcccg cagatttcag gaaggacgac
     3961 ggggaacagg gcggcccatc acctggagga gcagcttcaa atcctcccaa gcctcccgtt
     4021 tcatggccgg gtttcgcaat aaaaacgctg ggtgatacgt acagacaacc tttgcccgat
     4081 cgtattccca gaccttcccc cggagctttc cgatcccaac atccgttcgg agtaggtttt
     4141 gggcggccac gttccccagg caacagatgt atttcggctg gatcaccgcc aacgtggcat
     4201 ccagccacgg ccgacaacgg tcggcttcct cgcgggtcgg cgttcgattg ttgggcggac
     4261 gacagcgaag aatgttacaa atatacacgt cctcgcgtcg catccccatc ccgttggtta
     4321 tcatagcggt caataattgc ccggcccgac cgacaaaagg ctctccttgc ttgtcttcat
     4381 caacacccgg cgcctctccg ataaacacaa gctctgaata cggattgcct acaccgaaaa
     4441 ccgtctgcgt ccggtttgca accaattccg gacaacgtgt gcactgtgcg acctgcttcg
     4501 ccaactcggc caaaacatct tcacgacttt cggacaatgg ttggatctcc cccaacggtg
     4561 ccacggtatc caccacagga agctgcccag ttacgcggtt ttcctctgat ccctgccttg
     4621 cctgagcttc caacggctca ctttggccca gacgagtgtc atcagaactc acctgttggg
     4681 atcccaataa cgaagaatca atcgtctcgg cggacggcca aatgagatgg ttgatccccg
     4741 catgttgcat gtcctcaaga tactgaacaa gcccccgtcg cagacgttcg ggatcgagcg
     4801 acggcgaatt catcttgtct tgctgggagg ccgctttcat ggcaagcagg aatgagtaat
     4861 tctcaaaggt tccaaatcac ttctcccgcc gagtatcccc gttgggtaag gtgtaccacg
     4921 ctgggaatgc ctatgttgct ggtaagtcgg tcatgatgac caacggctgc ctttatgccc
     4981 tgcgtctttg ccgaagctgg gagacctgga gcttcgcctg ttgtgtgcgg tggcagatac
     5041 ggatgaagtt ctcgaccaag ctcacgcccg gcaacgtaaa gagcatgcga tcgccttcat
     5101 acacttggac atcgcccgcc tggagcgcca cttgcccggg gtgagtcacc acttccagtc
     5161 ggctgatctg ggaccaagga gcatcccaca ccactcgagc gataagcccc tcaacgagga
     5221 gcaggcgctc gctggtcacc agatacctcc gacaccgcct tggccgaagt cgccacaaat
     5281 agaccagggc cgccacaggt gccaaaaaga agccggcaca caatgcggcc aagggtccac
     5341 gggctagggc ccagccccat acccgaccca cccagcgtcc taacggccag gccgtaatgg
     5401 agggccaaac gaccttgacc acctgttcat tcggcatcaa catctcggtt ctacctggct
     5461 ataaaaaatt tgacttctct tggcaaatgg gttcgatcgg tccattgtac cgggctccgt
     5521 tttaaaagga gagggcagga tcagctttcg gcgccagcca gaccgagcat ctcttgcacg
     5581 agtttgtcga tctcagccaa ttcgtgcccc aaaagctcgc gaatctcctg acgcaataaa
     5641 ccaatccgtt tgctgaacca tgaaacgccc aggccgaggt ttgaagaaag gccgatggac
     5701 acttgagcca ctgccggtga ccgctcaagc aattcgctca gacggtggag agaaacctcc
     5761 gataatggcc ctggcgtgcg tctagatcaa cgagtggcga gagaacaacc atgaaaggcc
     5821 acgggaaggc ccattgatta cccgtagcgt tctccaaatt cgagtctatc ccaaaaagtt
     5881 cccctctccc tctgggagag ggttggggtg agggcaagcg atcgtcgaag tcgaacgaga
     5941 aaaccggcag cgattcgagc gaccctcacc cccgacccct ctcccacagg gcgaggggag
     6001 attgtgggat aggttctttg tcacctgcgt tttcaactga tggagctcac aaattgccgc
     6061 atttctccat ctaaaattac aatttacccc aacgcctatt tcgatcacga ggtaaactcc
     6121 cgcggggtat ccctcggcag aagaccgaac ctagatggag aaaatggccc gacgacagcg
     6181 cctgacagtt ggatgaaaga aatgccacgg ttattgggga gcgatttcgc cggggccgag
     6241 tccctgttca tcacgccacg gtacgtcgat ggtcttcttg attctatttt
//
LOCUS       DAOSUT010000206         6277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5836_length_6277_cov_10.366442, whole genome shotgun sequence.
ACCESSION   DAOSUT010000206 DAOSUT010000000
VERSION     DAOSUT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5836_length_6277_cov_10.366442"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..2931)
                     /locus_tag="PLD05_14180"
     CDS             complement(349..2931)
                     /locus_tag="PLD05_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOP78636.1"
                     /translation="MTRIALLAIDVTNCGPWRGHHRLEFPATGVAVFCAPNETGKTTL
                     LRLVAAVLWGAGVPECNWFAREDEPFAAAIEYERAALQPRHETEPPIRYRVERDFRTG
                     RVCLFRLEKGEWQSVQVTRHKRRGRTADSEQWMNLVQEVFAPISPRAFERLVVLSPPF
                     NPQPEGELVQSLISGAGESTKDEALETLLERYRAITRYSKKEGLSTSDARKDGRLDEL
                     RRRRDELQRDCELAKEMLEKGLRLREEIQRLDEQVAAKEEKGNELQRNKGILDKTRRL
                     SAERRTKEELAGNLARALDQWKKLEDSAREVREQLQRFPAFLRDSTSERRKYWAEALR
                     KYQERARRLIEQSRVVNEEVLKERFADVWDWPADAPSRVEQIQKALALCHEAEQHHRE
                     AVSRAASIRPVLDRKRQMPVVIGVAAAVMLVLVLILGLFGYLVMGLLVGVIAAIAGGL
                     ITAQIYRPECWPPEYAQWQQKVAQAEGDHEKCVRALEKYWEEIARWAQTRELPILLER
                     LGRFKSLCDAKRQLLNQKAELERMAAQLTVRAIPKELTDMCGLESEAADSAPLGPDVF
                     ERGLSLLNDYEKLVAQEQSYRQRQEIVLHSVGVDTIESLQERHRQAEGDLQGIILESR
                     KLAQDSPLAEEAFSWDARRLENEYRRLEEELTRLETDLREIREQRNDRERELARWEGQ
                     NVVNLAQAQDELNMVEREITILEGRARAIATASRLIEEAYSVFSHQHRVAIQHGVNSI
                     MSKWTGLDEREFLVDENFVVRFRVPSLGGNGNNQWKCEHLSQGAQDQLALAIRWAVLD
                     RLAGDVVLPLLLDDCFHMWDAQRRDSLRSFLQEHRQRQIILVTHDERFLDWGEPVRRV
                     SSEI"
     gene            complement(2940..4133)
                     /locus_tag="PLD05_14185"
     CDS             complement(2940..4133)
                     /locus_tag="PLD05_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOP78637.1"
                     /translation="MRDTILHLADLHLDASPEARLDDAVRAWLLGARRRILNALASWI
                     ARDESRIGLVLIAGDLFDRHDPPQEVVSEARTALAEIARLVPVVTVPGNHDEYSYAQC
                     VYRRGDWPGVLVTSPEPQLVWKGQLGDSVCAVVSAAYQAGKVSPGQRLRFPSRSQVMG
                     GDDSNGVLIGLFHSTLADHFPDEFVRNERCFVLSHQEAANAGYDYLALGHFHTRREWR
                     IGACLAHYPGPPLGARIADPGSGQLSLVRIRNGTVTLEGLSAGPMVGCQWMFLDVELQ
                     PEEKPREVVERIADRCRNVEANSGIAAIHVARLKGNTIHDNLAEQIQNELRAKGVSCT
                     VVAHELEEILSPDIEAMAAEESLVGYFVRSWQEWQSRQEVSRAEAMAVLYEGLLALGW
                     HRANN"
     gene            4780..5718
                     /locus_tag="PLD05_14190"
     CDS             4780..5718
                     /locus_tag="PLD05_14190"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOP78638.1"
                     /translation="MLHAVSRRDFLYYGAATAGTMALGNRFGWALENAAPKMVITRWT
                     GPMPQSPSEFQQAVVKLTEQTVQAMGGISRFVKRGDVVWIKPNIGWDRTPELAANTNP
                     DLVATLVRLCYEAGAKRVKVGDNPCDLPAKSYASSGIADAAKKAGAEVIFLDRERFRE
                     TDIKGERVKSIPVYPEIVESDVVINVPIAKHHVLATVTLCMKNYMGVIENRRLFHQDI
                     PTCLADITRFMRPTFCILDCTRIMVNHGPKGGNLADVQVKLTVAAGTDIVALDAFGAE
                     ILGRQPSQIGSIVKGQQAGLGTMDYRSLQPKEIALS"
     gene            5715..>6277
                     /locus_tag="PLD05_14195"
     CDS             5715..>6277
                     /locus_tag="PLD05_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOP78639.1"
                     /translation="MKRRIAPRRWVRRVVQLVFLTLFVGLVLATRPVPGQLPSDWLKV
                     FFFFDPLILVLTGIAAWGIPTLALLGIVTLVVTALLGRVFCGWICPLGTVHAMTGWAL
                     DKVQKAKPGRDIWSPWQKTKYYVLIAALVMALFGGHWITIFDPLVLFYRTLTTALIPS
                     GQWAIESGATAIYKANPRIGSFAVTDL"
BASE COUNT         1320 a         1894 c         1667 g         1396 t
ORIGIN      
        1 gaggggtcgg gggtgagggt ggctcgaatc gctgccggtt ttctcaatcg attctgacgt
       61 ccgcttgccc tcacctcaac cctctcccgg agggagaggg gggacttttt cgcgagaggc
      121 gctaagacaa acgacgaggc ccatcctgta tgaataaagt ataacgattc gcgaaacgat
      181 gtggagaaat cacgtccagc cgacatcacg ggccgtcagt gcaagctggc gggtcgtcat
      241 acgttgctgg cagttggaaa acctgcggcc aataaaaagc aactcattct acagcgggag
      301 cccgccacat tgaaacccga gacgaatttc gattatcaag ggcgctcgtc atatttccga
      361 ggacacgcgc cggacaggtt ctccccaatc aaggaaacgc tcatcgtggg taaccaggat
      421 aatctgccgc tggcgatgtt cctgtaagaa gcttcgcaga ctgtcgcgtc gctgggcgtc
      481 ccacatgtga aagcagtcat ccaaaagaag tggcagcacc acatccccag ccaagcgatc
      541 gagcaccgcc caacggatgg ccagggcaag ttggtcctgg gccccttggc tcaggtgctc
      601 acacttccat tgattgttcc cattgccacc caagctggga acgcggaatc tgacgacaaa
      661 gttttcatcc accaaaaact ctcgttcatc caaccccgtc catttgctca taatgctatt
      721 gacgccgtgt tggatggcca cgcggtgctg gtgcgaaaac accgagtaag cctcctcgat
      781 aagacgcgag gccgtggcaa tcgcgcgggc ccgcccctcg agaatagtta tttcccgttc
      841 aaccatattg agttcgtctt gagcctgggc aagattcacc acattttgcc cctcccaacg
      901 cgccaactcc ctctcacgat catttcgctg ctcgcgaatc tctctaagat cggtctccag
      961 ccgggtaagc tcctcctcga gacgtcgata ttcgttttct aatcttcgag catcccagga
     1021 gaatgcctct tctgcgaggg ggctgtcctg cgccagtttt cgcgactcca ggatgatccc
     1081 ctgtaggtcc ccctccgcct ggcgatgtcg ctcttgtaac gactcaatgg tgtccacgcc
     1141 cacgctgtgt aggacaattt cctgccgctg gcgataagac tgctcttggg ccaccagctt
     1201 ttcgtagtca ttcaagagac tgaggcccct ttcgaacacg tccgggccaa gtggggctga
     1261 atccgccgcc tcgctttcga gaccacacat gtctgtcaat tctttgggga tggccctaac
     1321 cgtaagttgg gcggccattc gctctagctc cgccttctgg ttgagtagct gcctcttggc
     1381 atcgcatagg cttttaaagc gtcccagccg ctccaacaaa atgggcaatt cccgcgtttg
     1441 tgcccagcga gctatttcct cccaatattt ctccaaggcc cgaacgcatt tttcatgatc
     1501 cccttcggct tgggccacct tttgctgcca ttgggcatac tccggtggcc aacactccgg
     1561 tcgataaatc tgcgccgtta tcaagcctcc cgcaatggcc gcgataactc ccaccagtag
     1621 gcccatgacc aggtagccaa acagaccgag gatcaaaacg aggaccagca tcactgccgc
     1681 agcaacacca atgacgacgg gcatctgccg tttacggtcc aggacgggtc ggatacttgc
     1741 cgcgcgggac accgcctcgc gatggtgttg ctcggcctcg tggcacagcg ctaaggcctt
     1801 ctgaatctgc tccacgcgcg aaggggcatc cgcgggccaa tcccagacgt cggcaaaacg
     1861 ttctttgagg acctcctcat tcaccacgcg cgattgttcg atgagccgtc gtgccctctc
     1921 ttgatacttc cgcaaagcct ctgcccagta ctttcgacgt tcgcttgtgc tgtcgcgaag
     1981 aaaggcagga aacctctgga gctgctctct cacctcccgg gctgaatctt ccaatttttt
     2041 ccactgatca agagcacgcg cgaggtttcc cgccaattcc tctttggtgc gacgttcagc
     2101 agaaagccgg cgcgttttgt ccaagattcc cttgtttcgc tgtaattcgt tgcccttttc
     2161 ttccttggcg gccacttgtt cgtccagccg ttggatttcc tcgcgaaggc ggaggccttt
     2221 ttccagcatc tccttggcca attcacagtc acgctgcaac tcgtctcgtc gtcgccgtaa
     2281 ctcatcgagc cgaccgtcct tgcgggcatc cgaagtactt aatccttctt tcttggaata
     2341 ccgcgtgatt gcccgatacc gctcgaggag tgtttctaac gcctcgtcct ttgtgctctc
     2401 gcccgccccg gagattaagg actgcaccaa ttcgccctca ggctgaggat tgaacggagg
     2461 cgaaagtacg acaagccgct caaaggcccg gggcgagatg ggggcaaata cctcttgcac
     2521 caagttcatc cactgttcac tgtcggcagt gcggccccgt cgtttgtgac gggtgacctg
     2581 cacggattgc cattctccct tttccaggcg gaaaagacaa acccggcccg ttcggaagtc
     2641 ccgctcaacc cgatagcgga tcggcggctc tgtttcatga cgcggctgaa gtgcagcgcg
     2701 ttcgtactca atagcggcgg cgaacggttc gtcctctctc gcgaaccaat tgcactcggg
     2761 aaccccggca ccccacaaca cggctgctac aagccgcaac agtgtcgtct tgccggtttc
     2821 attgggggcg caaaacacgg ccacacctgt ggcaggaaat tctaagcggt ggtgtccccg
     2881 ccagggccca caattcgtga cgtcaattgc caagagtgcg attcgcgtca tgtctgccct
     2941 caattgttcg cccggtgcca acctaaggcc aaaagccctt catagagcac ggccatggcc
     3001 tccgcccgtg atacctcttg ccgagattgc cactcctgcc aagatcggac aaaataaccg
     3061 accaaacttt cctccgcggc cattgcctcg atgtcgggag aaagtatttc ctcgagttcg
     3121 tgcgccacga cggtacagga aacccctttc gctcgcaatt cattctggat ctgctcggca
     3181 agattatcgt ggatagtatt ccctttaagt cgcgccacgt ggattgcagc gatgccgcta
     3241 ttggcttcca cgttgcggca ccgatcggca atccgctcga cgacttctcg cggcttttcc
     3301 tccggctgaa gttccacatc caagaacatc cattggcatc ccaccatggg accggccgaa
     3361 aggccttcca gcgtgaccgt cccgtttctg atccgcacaa gcgacaattg tccactgccg
     3421 ggatcggcga ttcgcgcacc gaggggcggc cccggatagt gggccaaaca cgcaccgatc
     3481 cgccactccc gtcgcgtgtg aaagtgcccc aaagccaggt agtcgtaacc ggcattggca
     3541 gcctcctgat gggagagaac gaaacatcgc tcattcctaa caaactcatc aggaaaatga
     3601 tcggcgagtg tgctgtggaa gaggccgatg agaacaccgt ttgaatcgtc cccacccatc
     3661 acctgacttc gtgacgggaa ccgaagtcgc tgaccaggcg acaccttacc agcctgatat
     3721 gccgctgaaa ccacagcgca caccgagtcc ccgagttggc ctttccagac aagctgtggt
     3781 tcgggcgagg tcacaagcac accgggccag tcaccccggc gatagacgca ttgcgcgtaa
     3841 ctgtactcgt cgtgattccc tgggacagtc acgacgggca ccagtcgagc aatctcggcc
     3901 agtgccgtgc gggcctcgga gaccacctct tgcggcgggt catgccggtc aaagagatcg
     3961 ccggcaatca gcaccaagcc aattcgcgat tcgtctcggg caatccaact cgctagagca
     4021 ttcagtatcc ttcgccttgc cccgagcagc caggcacgca ccgcgtcgtc cagccgggcc
     4081 tctggggatg catccaagtg aagatcggca agatggagaa ttgtgtcgcg cattgcccca
     4141 ttttcctaac agaaaaaaca caccgtcaag cggcacgcaa tcacctttta gcccggtctg
     4201 cccctaagtt cggatgtgtg cccaacccac catcgaaggc ccgaatggtt cgacagggag
     4261 gcaggcttat ttcggtttct taggccgagg taccacctga gcacaccgtt tacaccgcgt
     4321 ccgctgggtg ttcaattttg ccccacattt tgggcaacga cgattgtgca aaatgtggta
     4381 gaacttggtc cttggtcgtg ttcgcatagt aagatcgacg ttcttttccg tagagcctaa
     4441 caagattccg tcttgggaac atccgccatt cagcacattg gcttgactcg gaacacattc
     4501 aatgtaaaag gtgtcccgaa atgcgccagt gcccttcggt ccggcgaatc gacggtcgta
     4561 cataggcgcg tcgcagatca ctacccgcat acagcagcct gtggaggtgt caaacaacgt
     4621 cgcttttttt gtcataagga attgtcattg tgagtcgaat gaaataaagt agtaagacgt
     4681 gatgtcggtc attgctaaca actagccaac cgggcggaag tggccacggt ctattttgcc
     4741 cacctgttac ggtctcactt gccagaaagg atgacaccca tgttgcacgc agtatcacgt
     4801 cgtgactttt tgtattatgg agcagccaca gccggcacga tggctttggg aaatcgtttc
     4861 ggttgggcac tggaaaacgc tgcaccgaaa atggttatca cccgctggac cgggcctatg
     4921 ccgcagtccc cgtccgagtt tcaacaggcc gtcgtcaagc tgaccgagca aacggtccag
     4981 gcaatgggtg gtatttcacg ctttgtcaaa cggggtgacg tcgtgtggat caagccgaac
     5041 atcggctggg accgcacacc cgagcttgcc gccaatacca acccggactt agtggcgacg
     5101 ctggttcgcc tgtgctacga ggccggcgca aagagggtca aggtcggcga taacccgtgc
     5161 gatctacccg ccaagtcata cgccagtagt gggatcgccg atgccgccaa gaaagcagga
     5221 gccgaggtca tctttctcga ccgtgaacgg ttccgcgaaa ccgacatcaa gggtgaacgg
     5281 gttaagagta tccctgttta tccggaaatc gtggaaagtg atgtcgttat taacgtcccc
     5341 attgccaaac accacgtgct ggcgaccgtc acattgtgca tgaaaaacta catgggcgtg
     5401 attgaaaatc gccgtctctt ccatcaagac atccccacat gtttggccga tattacccgg
     5461 tttatgcggc cgaccttctg catcctggat tgcacccgga ttatggtgaa ccacggacca
     5521 aagggtggca atttggcgga tgtgcaagtc aagctgacgg tggcggcagg gaccgacatc
     5581 gtggcccttg atgccttcgg cgcggagatt cttggccgcc agccaagtca aatcggctca
     5641 attgttaagg gtcaacaggc tggattaggc accatggact accgctcgct acagcccaag
     5701 gagatcgccc tttcatgaaa cgtcggatcg cccctcgacg ctgggtgcgg cgggtcgtcc
     5761 agttggtgtt tctgactctc tttgtcggcc tggttctggc gacgcggccg gttccggggc
     5821 agttgcccag cgattggtta aaggtgtttt tcttttttga cccattgatc ctggtcctca
     5881 ccgggattgc ggcttggggt attcccacgt tggccttgct ggggatcgtc actctggtgg
     5941 taacagccct cctgggccgc gtcttttgtg gttggatttg ccccttgggg accgtccatg
     6001 ctatgacggg ttgggcgctg gacaaggttc aaaaggccaa acccggccgc gacatttggt
     6061 ccccatggca aaagaccaaa tactatgtgc tcatcgcggc cttggtgatg gccttgttcg
     6121 gcggacattg gatcacgatc ttcgatcctc ttgtcctctt ttatcggacc ctcaccacag
     6181 cgttgattcc cagcggacaa tgggcaatcg aaagcggggc aacggccatc tacaaggcca
     6241 atccacggat cggctccttt gccgtaacgg atctcag
//
LOCUS       DAOSUT010000207         6215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5904_length_6215_cov_10.140260, whole genome shotgun sequence.
ACCESSION   DAOSUT010000207 DAOSUT010000000
VERSION     DAOSUT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_6215_cov_10.140260"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLD05_14200"
     CDS             <1..1116
                     /locus_tag="PLD05_14200"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOP78640.1"
                     /translation="HTTAGYNLYAKKTMEWVFDVRDEDVYWCTADIGWVTGHTYIVYG
                     PLSAGVTTVMYEGAPNWPAEDRWWDIIERYRVNILYTAPTAIRTFIKWGDEHVEKHDL
                     SSLRLLGSVGEGINPEAWIWYYEKIGGGRCPIVDTWWQTETGGIMISPLPGCTPLKPG
                     SATLPLPGVVPEIVSEDGKPVPVGTGGWLVITKPWPGMLRGIWGDDERYKRQYWSDIP
                     HKYLAGDNARIDEDGYIWIMGRIDDVLNVSGHRLSTIEVESALVSHPAVAEAAAVGRP
                     HEVKGEAICAFVTLAEGYEPSEQLRQELKNHVRKEIGALAVPDDIRFTTALPKTRSGK
                     IMRRLLRDIAAGRETVGDTSTLEDYSVLARLRAGEEG"
     gene            complement(1250..2107)
                     /locus_tag="PLD05_14205"
     CDS             complement(1250..2107)
                     /locus_tag="PLD05_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOP78641.1"
                     /translation="MKLVTYKGPLGPRVGILREGKVLDLQQNEPELPTDIIALLRMGE
                     RAKPLLERVLQRGTVLETEVKRLAPIPHPQKVICVGLNYSDHARETGATPPPEPVIFN
                     KFPTAIIGPEEPIRLPRESANVDYEAELVVVIGRAGRRIPENEALDYVAGYMCGNDVS
                     ARDWQMQKPGGQWLLGKSFDTFAPTGPYLVTRDEISNPHALGVRLILNGQVMQQSNTN
                     QFIFNIPQLIAYVSQVCTLEVGDLLFTGTPGGVGFARKPPVFLRDGDVVEVEIEGIGT
                     LKNVVVADR"
     gene            complement(2528..3340)
                     /locus_tag="PLD05_14210"
     CDS             complement(2528..3340)
                     /locus_tag="PLD05_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78642.1"
                     /translation="MKYRVVVMAAGALLMMGMVAETASAGLLSRRPGLFQRLACRRVS
                     PAVAALQQERAKLEQAKQEAEEAKAAAEAAKKEAEAVKEAVEKALAAVKEEAAKALAQ
                     IRQETEAALKAKAEAEAASAKAKADVAAAEKAKAEAADLTAKAKALMEQAQAEMAKVE
                     AAKADLEKARQALQAEQQKLEAAKEDLAKQQAAVEEARKQAEALKAEAEKIKAEATKP
                     AEPTGQEAAPPSNESAAPAQSTQSAENASGAEAGSAGASAQGSPDTSTAATQ"
     gene            3691..5052
                     /locus_tag="PLD05_14215"
     CDS             3691..5052
                     /locus_tag="PLD05_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOP78643.1"
                     /translation="MKTYFKRTHTVLGHLSAWCVRWIASVGCAIAVFISSMTSADDWP
                     QWRGPNRDGIWRETGITRQFSGPEVKLRWRTKIAGGYSGPTVADGRVFVTDRLTEPEE
                     VERVHAFDWQTGQCLWTFSYPCVYRNVTYRTGPRASVTVHEGLAYALGTMGDLHCLDA
                     ETGTVVWKKRAGQDWEVRVPTWGVAAAPLVEGDLLIVQIGLEDGCVVALDRKTGELRW
                     KALPDQASYSAPITITQAGRRVVVCWTGERLAGLDALTGKLLWDVPFPHRRWIDGIIT
                     PVWDSSTNRLFISCFDCGSLMLHVDSNDLHVQKIWRRQGQNEINTDSLHILMGNPVIK
                     ENWIFGVDSYGQFRCLDARSGDRIWEDLTLTSQLRWGTLHMVQQADVVWMFNDRGELM
                     ITELTPQGVHVISKARLLAPTRGQLSRGPGVTWSHPAFAHRHVFNRNDEELVCADLSE
                     GAQ"
     gene            5516..>6215
                     /locus_tag="PLD05_14220"
     CDS             5516..>6215
                     /locus_tag="PLD05_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78644.1"
                     /translation="MVRRAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCTNN
                     LKQIGLALHNFHDVYGVFPVGQPDDDNDNYSWSAYILPFMEQKSMYDTLVGGGAALVY
                     IPGGDNLKVHGAIREIPGVSPALPSGHPVRNTDSYDWWTQVKSDHGNPNHANNVGAKS
                     VINSYICPSDVLPKQDNNGYGKSNYCCSLGSDEPWVNQYMSGGLSWSEPSASLQNGMF
                     RLAQTNDYHFVTTFS"
BASE COUNT         1305 a         1744 c         1745 g         1421 t
ORIGIN      
        1 cataccacgg ccggttataa cctgtatgcc aagaagacca tggaatgggt attcgacgtg
       61 cgcgatgaag atgtctattg gtgcacggcg gatattggtt gggtcacagg gcatacctat
      121 atcgtgtatg gcccgctgtc ggcaggtgtc accacggtga tgtatgaagg tgctccgaac
      181 tggcctgccg aggatcgctg gtgggacatt atcgaacggt atcgcgtcaa catcctttac
      241 acggccccca cggccattcg cacgttcatc aagtggggcg atgaacatgt cgaaaaacac
      301 gatctctcga gcctgcggct gctggggagt gtgggtgaag gaattaaccc ggaagcctgg
      361 atttggtact acgaaaagat cggtggtggt cgttgcccaa ttgtggatac gtggtggcaa
      421 accgagaccg gaggcatcat gatctcgccg cttcctggtt gcacgccact caagcccggc
      481 agcgccaccc tcccattgcc tggcgttgtc cccgagatcg tgtcagagga tggtaaaccc
      541 gttccggtgg gcaccggcgg ttggcttgtc attaccaagc cttggccggg gatgttgcgc
      601 ggcatttggg gagatgacga acgttataag cggcaatact ggtccgacat cccccacaag
      661 tacctagccg gcgacaatgc ccgaattgac gaggacggct acatctggat catgggccgc
      721 atcgacgatg tgctgaatgt gtcgggtcac cgactcagca ccattgaagt cgaaagcgcc
      781 ctggtcagtc accccgccgt ggcagaagct gccgctgtgg gacgtcccca cgaagtcaaa
      841 ggcgaggcca tttgcgcctt cgtgaccctc gctgaggggt atgaaccgag tgagcaactt
      901 aggcaagagt tgaagaatca cgtgcggaaa gaaatcggtg ccctggccgt gcccgacgat
      961 atccgcttca ccacggcctt acccaagacg cgaagtggta agatcatgcg gcggctcttg
     1021 cgtgatatcg ctgcaggacg cgaaaccgtt ggcgatacca gcacgctgga ggattacagc
     1081 gtcctagcac ggctccgtgc cggcgaggaa gggtaacgcc caccacagct taacaccttt
     1141 tacgaggtga acgaacaaac gacccgtggt ctagcgatca cgggtcgttt tttgttattt
     1201 ggaaaaagaa gaagcaggtc cccaattgcc ggcaatcgct cgccaggttt caccgatctg
     1261 caacgaccac atttttcaag gtaccgatcc cttcgatctc gacttcgacg acgtcgccat
     1321 cccgcaggaa aaccggtggc ttgcgggcaa aaccaacacc gccgggcgta ccggtgaaga
     1381 gaaggtctcc tacttccaac gtgcagactt gggagacata ggcgatcaac tgcgggatat
     1441 taaagatgaa ttggtttgtg ttggactgtt gcataacctg gccattgaga atcagtcgca
     1501 ctcccagcgc gtgcggattg ctaatttcgt cacgtgtcac caaataggga ccggtgggcg
     1561 caaaggtgtc gaacgatttt ccgagcaacc actgtcctcc gggcttctgc atctgccaat
     1621 ctcgagccga cacatcattg ccgcacatat aaccggccac gtaatcgagg gcctcgttct
     1681 cggggatgcg ccgtccggcc cggccaatta ccaccaccag ttccgcttcg taatcgacgt
     1741 tggccgattc tcggggcaaa cggatgggtt cttctgggcc gatgatcgcc gtggggaact
     1801 tattgaagat cacgggttcc ggcgggggcg tcgcccctgt ttcccgtgcg tgatcactgt
     1861 aatttaaccc aacgcaaata accttttgtg gatgaggaat gggggccaac cgttttacct
     1921 cggtctccaa aacggtacct cgttgaagta ccctttccag gagcggcttt gcccgttcgc
     1981 ccatacgaag caaagcaatt atgtccgttg gcaattccgg ttcattttgc tgaagatcga
     2041 gcaccttccc ttcgcggaga atccccacgc gcggaccgag gggacctttg tacgtgacca
     2101 atttcatgac atcttctccc tggtttcggt taccaatatc cggatcattt cactcagagt
     2161 ctatcccaga aagtccccct ctccctccag gagaaggttg gggtgagggc aagcgatcgt
     2221 cggaatcgat cgagaaaacc ggcagcgaat cgagccaccc tcacccccga cccctctccc
     2281 gccgaacggg agaggggaga ttataagata ggctcttaat ggtgaggcat ccatccgttg
     2341 agtaatcaaa tgagtataac ggctcgtctg acattctgca gtcctcctgc gacacgtgca
     2401 caatcacaag ttgcagtaat ctttcgctca cgatcgacgg caccaacaaa aaaccggtct
     2461 gaaacagcgt gttccagacc ggtcctgcgg gatagtcccg atgccgacgg tttagatcaa
     2521 agatgcttca ctgggtagcc gccgtcgacg tgtcgggcga tccctgcgcc gaagcccccg
     2581 cagagccagc ttcggcgccg gaggcatttt cggccgattg ggttgactgg gctggtgccg
     2641 ctgattcatt cgatggaggg gcagcctcct gacccgtcgg ttcggctggc tttgtggctt
     2701 cagccttgat cttttcggct tccgctttca aggcctcggc ctgtttgcgc gcctcctcga
     2761 cagcggcctg ctgcttcgcc aagtcctcct tagcagcttc taacttttgc tgctctgcct
     2821 ggagtgcctg gcgggccttc tccaaatctg ccttggcggc ctcaaccttt gccatttcgg
     2881 cctgggcctg ttccatcagc gcttttgcct tcgcggtgag atcagcagct tccgccttcg
     2941 ccttttctgc cgcagccacg tcggctttgg cctttgcgga agcagcctcg gcctcggcct
     3001 tcgccttcag ggctgcttcc gtctcctggc gaatctgggc caacgccttg gcggcctctt
     3061 ccttcacggc agccaatgcc ttttcgacgg cttctttcac ggcctcggcc tccttcttgg
     3121 cggcctctgc cgctgctttc gcttcttcag cctcctgctt tgcttgctcc aacttggccc
     3181 gctcttgctg aagagcggct acggctggac tgacccgccg gcacgccaac cgctgaaaca
     3241 ggccggggcg tcggctcaac agtcctgcgg aggcggtctc ggcgaccatt cccatcatga
     3301 gaagggcccc cgccgccatg acgacaactc tgtacttcat gcggtgtcct ccttttcaat
     3361 caaaaccccg gcgaagccat ctgtgaggac caccctcaac atcaccaagg ggcttcgcac
     3421 tacttagtta acctcagatg caggctactt accatcagaa cgtaggctgc caaaattatc
     3481 gaatatgtgg gtcggcaaat aaacaggggc tgggttattg cccccactct ggggaaaaca
     3541 tgacgcacgc ggaattgccc ggcggtatgc cctaactttc ccgctccctc aatttttagg
     3601 ataattgccg atcacaacga ttgcacgatg ggcaagcagc acgcgtggtg tcattcgatc
     3661 tttccatccg actgtgggag gccgtccacc gtgaaaactt actttaaaag aacacacact
     3721 gtgttggggc acctctcggc ctggtgcgtt cgctggatcg cctcggtggg ctgcgctatc
     3781 gccgttttca tatcttcaat gacctccgcc gatgattggc cgcagtggcg ggggccaaac
     3841 cgagatggca tctggcgaga aacgggcatc acccgacagt tttctggacc ggaggttaaa
     3901 ttgcgatggc ggaccaagat cgcgggcggg tatagtggtc caacggtggc cgatggccga
     3961 gtctttgtca ccgatcgact caccgagccg gaagaagtcg aacgtgtgca tgccttcgac
     4021 tggcaaacgg ggcagtgtct gtggaccttc agctatcctt gtgtctatcg aaacgtcacg
     4081 taccggacgg gccccagggc ctccgtgact gttcatgaag ggctagccta cgccctgggg
     4141 accatgggtg acctgcactg tctggacgcc gaaacgggca cagtggtctg gaaaaagcgg
     4201 gctggtcaag attgggaagt tcgtgtcccc acgtggggtg tcgcggctgc gcctctcgtg
     4261 gaaggcgacc ttctcatcgt ccaaattggt ctcgaagatg gctgcgtcgt cgcgctcgac
     4321 cgaaagacgg gcgaacttcg ctggaaggcc ctcccagatc aggcctcgta ttctgccccg
     4381 atcacgatca cccaggccgg acggcgcgtc gttgtgtgct ggaccggcga acgccttgcg
     4441 ggactcgatg cccttacggg aaaactactg tgggacgtac ctttccctca tcgacgatgg
     4501 attgatggca tcatcacacc tgtctgggac tcaagcacga accgcctatt tatcagttgt
     4561 tttgattgcg ggtcactcat gttacatgtt gattctaatg acttgcatgt gcagaaaatt
     4621 tggcgacgcc agggccaaaa cgaaattaac actgattctt tacacatact catgggaaat
     4681 ccggttatta aagaaaattg gatttttggc gttgacagct atggacaatt tcgctgcttg
     4741 gacgccagga gtggcgatcg gatctgggag gacttgacac ttacctcgca acttcggtgg
     4801 ggaacgttac acatggtgca gcaggcggat gtggtctgga tgttcaacga tcggggcgag
     4861 ctaatgatca cagaattaac gccacaaggt gtccacgtca tttctaaggc acggctactg
     4921 gcccccaccc gtgggcaact aagtcggggc ccgggcgtca cgtggtcgca tccggcattt
     4981 gcccacaggc acgtttttaa ccgaaatgac gaggaacttg tgtgcgccga tttgtccgaa
     5041 ggggcccaat aaagcgagtg tttttccctt gcaatgccga acgtcctcca agtcatcctt
     5101 gcctttttag tccccagtac cgagtatgtc cggtggctaa aaaaggaacc cggcggaact
     5161 tttttcgctt cagattgttc acaaataagg cggcatatac tataatccaa atagaaggcg
     5221 catgacatgc ggctcttctc ggaggttaac ggccgggaag acgtgcggct ggccagcagg
     5281 tcggaagata ggtttcgaaa agggattctt ccgatccatc ccattttgtg tgcgcgtcgg
     5341 caaggggcgg tgccgcgagt agttccccac ccttggcacc gaggctgagg cgttgatttt
     5401 ttgtgcaatt cagggcaggc aacgcagggt gttggttgga tcgttagtgt tcaaatgttc
     5461 agtttggtct cgtttgctca ctctttggag gtgtttcatg aaaggttcaa atcgcatggt
     5521 gagacgagcg tttacgcttg tcgaattgtt agtggtgatt gccatcatcg gaattttgat
     5581 tgccttgctt ttaccggcgg tccaggctgc gcgagaggct gcgcgacggt cacagtgcac
     5641 aaacaatctc aagcaaattg gcttggcctt gcacaatttc cacgatgttt atggggtgtt
     5701 tccggtaggt cagccggatg atgacaatga caactactcc tggagcgcgt acatccttcc
     5761 gtttatggaa cagaagtcga tgtatgatac ccttgtgggc ggcggcgcgg ccctcgttta
     5821 catccccggc ggcgacaacc ttaaagtcca tggtgcgatt agggaaattc ccggtgtctc
     5881 gcccgcccta ccgagcggcc accctgtgcg gaataccgac tcgtacgact ggtggaccca
     5941 ggtgaaaagt gatcatggca accctaacca cgccaacaat gttggtgcca agagcgttat
     6001 caacagctac atttgcccca gcgatgtttt gccgaagcaa gacaacaacg gttacggaaa
     6061 gtccaattac tgctgtagcc tcggcagcga tgagccatgg gttaatcaat acatgagtgg
     6121 aggacttagc tggagcgagc catcggcgtc tcttcaaaac gggatgttcc gattggccca
     6181 gacaaatgac taccatttcg tgacaacctt ctcag
//
LOCUS       DAOSUT010000208         6214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5906_length_6214_cov_10.193051, whole genome shotgun sequence.
ACCESSION   DAOSUT010000208 DAOSUT010000000
VERSION     DAOSUT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_6214_cov_10.193051"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..491)
                     /locus_tag="PLD05_14225"
     tRNA            complement(421..491)
                     /locus_tag="PLD05_14225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Gln,seq:ttg)
     gene            complement(880..2232)
                     /locus_tag="PLD05_14230"
     CDS             complement(880..2232)
                     /locus_tag="PLD05_14230"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78645.1"
                     /translation="MSRPHDIPRRTFLGQSAKAGTLGVAVPMFVSSTVLGEEGSGGAN
                     EKVRVGLIGCGGMGQANLANTAQHPDVVVAAAADPWEQRLNAVLKKYPGAKGFKDYRQ
                     LLEQKDVDAVIIATPPHWHALQAIHACEAGKDIYLQKPMTLHLGESLAVRNAVKRHQR
                     ISQIGTQIHASENYRRVVEKIRAGYIGKVSTVRTFNVMNQGPEGIGHDPGTPCPPDLD
                     WEMWIGPAPMRPYNKILAASAYHHGSWMDYSGGWTPGMAPHIIDLPIWALDLHYPIRV
                     HCSGGRYIIQDDGDAPDVQEILWQYPNLTMTWMGNLVNSYGFDFHGEPVPKRRLGIYF
                     HGVDGTLYANYSMHEVVPEGDRMKDRKPPEPSIPPSPGHEIEWVNCIKTREQPSCSVF
                     YHVKVDVPIVLGNLAYRLGRSIQFDPEKEQIVGDEEAARLAVPTYRAPWKFPEEYLKG
                     "
     gene            complement(2429..3898)
                     /locus_tag="PLD05_14235"
     CDS             complement(2429..3898)
                     /locus_tag="PLD05_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78646.1"
                     /translation="MARLSRRTFIKSSALASTAFSFAISGTRSSGQIIGANETINIGV
                     AGIRGRGSSHIDAFCAMPNVRVTYLIDPDSRLFAGRIKQVETRGGNTPKCVQDVRKAL
                     DDKDLHAISIATCNHWHSLMTIWACQAGKDVYVEKPCSHNVFEGRKCVEAARKYGRIV
                     QHGTQSRASAGWARQVAAIASGKYGKLLVSKGYASKPRWTIGFKPVKDPPPELDYNLW
                     LGPAPQLPYHENLVHYNWHWFWPTGNGEIGNQGVHQMDIARWAIPGATLPDWVISAGG
                     RFVDEPQHNWKDQGETPNMQITVMGFGDTILLFEVRGLVGKAGPGGKKFDPIVDNEFY
                     LEEGVIRNGKFYPKGKDQPEDLVKVDIKMGPGGQFENFIACMRSRKVEELHADIEVAH
                     YSSALCHLGNISYRLGKEVSFAEAAKGLPDHELVAAAWKMLEENIQGALGVELTKLTC
                     ILGPKLAFDPKKEKFVNNPEADKLLTRDYRPPFVVPENV"
     gene            complement(4963..6213)
                     /gene="hydF"
                     /locus_tag="PLD05_14240"
     CDS             complement(4963..6213)
                     /gene="hydF"
                     /locus_tag="PLD05_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOP78647.1"
                     /translation="MTGYRPAPKSFRLHIGIFGRRNAGKSALLNMLTRQEVSIVSDVA
                     GTTTDPVEKPMEMLPLGPVLFIDTAGVDDEGALGEMRVQKTRQVFDRTDLALLVCDAG
                     RWEAFEEQLLQEFRQRRVPVIVVFNKMDLALPNEALCRRFEQEKIPFVFTSALRREGL
                     EAVREALIRAAPEDFINSPPLIRDLIEPGDLVVLVIPIDKEAPKGRLIQPQVMTLREI
                     LDHGAMALVVRDTELAQVFQQVPRKPSLVVTDSQAFQHVANVTPQDVSMTSFSILMAR
                     QKGDLNEFVRGALAIESLRTGDHVLVAEACSHHPITDDIGREKIPRWLQQHVGGPLRF
                     TTVQGHDFPEDLSNYRLVIHCGACMWNRREVLSRIERCRRAQVPISNYGVTIAYTLGI
                     LDRALEPFAEARELFRRLIPQEVS"
BASE COUNT         1398 a         1724 c         1778 g         1314 t
ORIGIN      
        1 aggagtgtat ccgagcgacg attccctgca aaccacagcg gatgtgtaga tgataaggga
       61 ttgggctgca gtgtatccga gcgacgattc cctgcaaacc acagcagtat gcgacgcggg
      121 acgaggctat gattgagtgt atccgagcga cgattccctg caaaccacag caataacgca
      181 cgcctgggca cggtggcctg aagtgtatcc gagcgacgat tccctgcaaa ccacagcagc
      241 ccaacgaccc aggttaatgg agcacgcagg tgataacgag cagtacctat cggcagaagg
      301 gacgagcaaa ctgggcaaac gtgacggacc gcagatgtgc ccctttttga gaacggatag
      361 tctctcgtca catacccgtt gagttatata ccccctgtgc ccgaagaact atctcccaag
      421 ctacccggcc aggattcgaa cctggaatta gggagccaaa gtcccttgtg ttaccgttac
      481 accaccgggt agcagaacgg actacccgac gtcgcaggcc gggccccgtc taattccatt
      541 tttagcgtca ggtgcaggtc ccgacaagac cggcttggtc ttgcggtcag ttccccgcgc
      601 ccggggcgtt tcgatttcgc acggacccgg gcgttctttg tgttctgggg caggcacagg
      661 ggcctgcccc tacagggttt caatgggctc ctgttcgccc agaaccccag gagcgccagt
      721 ttagagccta tcccagaatt ccctctccct ttgggagagg gttagggtga gggcaagcca
      781 tcgtcgaagt ggatcgagaa aaccagcagc gattcgagcc accctcaccc ccggcccctc
      841 tcccgccgaa caggcgaggg aaggttgtgg gatagcctct tagcccttca aatattcttc
      901 cgggaatttc cacggggccc gataggtggg cacggcgagc cgggcggctt cttcgtcacc
      961 cacaatctgc tctttctccg gatcgaattg aatggaccgt cccaagcggt aggccaggtt
     1021 ccccaggaca atggggacat cgaccttaac gtggtaaaag acactgcacg agggttgttc
     1081 gcgggtcttg atgcaattga cccattcgat ttcgtgtccg ggtgacggcg ggatagaggg
     1141 ttccggcggt ttgcgatctt tcatccgatc gccctctggc acgacttcgt gcatcgagta
     1201 gttggcgtac agggtgccat ccaccccgtg gaagtaaatc cccaggcggc gtttgggaac
     1261 cggctcgccg tggaagtcga acccatagct gttgaccaga ttgcccatcc acgtcatggt
     1321 caaattggga tattgccaaa ggatttcctg aacatccggg gcatcgccgt catcctggat
     1381 aatgtagcga ccgccggaac aatggacgcg gatggggtag tgcaagtcga gggcccagat
     1441 gggcaggtcg atgatgtgcg gggccatgcc aggcgtccaa ccgccgctat agtccatcca
     1501 ggaaccatgg tggtaggcgc tagcggcaag gattttgtta tacggacgca tgggggcagg
     1561 gccgatccac atttcccaat ccaggtctgg tgggcaaggc gttcccggat catggccaat
     1621 accttccggg ccttgattca tgacgttgaa cgtgcggacc gtgctcacct tgccgatata
     1681 gccggcgcga atcttttcca ccacgcggcg ataattttcc gaggcgtgga tttgtgtccc
     1741 gatctgactg attcgctgat gccgtttgac ggcattgcgc acagcaaggc tttcaccaag
     1801 gtgaagcgtc atgggctttt gcaggtagat atccttgccc gcttcgcagg cgtggatcgc
     1861 ttgcagcgca tgccagtggg gcggtgtggc aatgatgacg gcgtctacgt ctttttgttc
     1921 gaggagttgt cggtaatcct tgaatccctt ggcccccggg tatttcttga ggacggcatt
     1981 caatcgctgt tcccaaggat cagccgccgc tgccacgacg acatcgggat gttgggctgt
     2041 gttagccaaa ttggcctggc ccatcccacc gcaaccgatc aggcccacgc ggaccttctc
     2101 attggcacca ccactgcctt cctcgcccaa cacggtactg gaaacaaaca tgggcacggc
     2161 cacgcccagc gttcctgctt tggccgactg accgagaaag gtacgccggg gaatatcatg
     2221 cggtcgagac atggtaaaga ctcctcttta aaaacttcag gcaactgacc acgaaccgaa
     2281 tatccccggc gcggacggcg gaaaacggat gacccgcacc ttgccgtcgc aatacgtagc
     2341 ctagcctgag gcccatggga cccaggcggc ctgacctgtc tcggtacgct tcgagctatt
     2401 cctaaatgcc gcgcttgctg gcgtttggtt acacgttctc aggcacgaca aagggcgggc
     2461 gatagtctcg ggttaacagt ttgtcggcct cagggttgtt aacaaatttt tctttcttgg
     2521 gatcgaaggc cagctttggc cccaggatgc atgtgagctt cgtcagctcg acacccagtg
     2581 caccttggat gttctcctcg agcatcttcc aagcggcggc cacaagttca tggtcgggca
     2641 gccccttggc ggcctcggca aaggagacct ccttcccaag ccggtaggag atgttgccca
     2701 aatggcacaa ggccgaggaa taatgggcaa cctcgatatc ggcatggagt tcctcaactt
     2761 tgcgacttcg catgcaggca atgaagtttt cgaactggcc acccggcccc atcttgatat
     2821 cgactttgac gaggtcttcc ggttgatcct tccctttggg gtagaactta ccgttgcgga
     2881 tcacaccttc ctcgaggtag aactcgttat caacgatggg gtcgaacttc ttgccgcctg
     2941 ggccggcctt tcccacaagc cctcgcacct cgaagagaag gatggtatca ccaaagccca
     3001 tcacggtgat ctgcatattg ggcgtttcgc cctggtcttt ccaattgtgc tgaggttcat
     3061 cgacgaagcg gccgccggcg ctgatgaccc aatcgggcaa ggtggcaccg gggatggccc
     3121 aacgggcgat atccatttgg tggacgccct ggttgccgat ctcgccgtta cccgttggcc
     3181 agaaccagtg ccagttgtag tggacaaggt tttcatggta aggcaactgc ggcgcgggac
     3241 ctagccacaa gttatagtcg agttctggcg gaggatcttt cacaggcttg aagccaatcg
     3301 tccatcgcgg cttgctggca taaccctttg acacgaggag tttgccatac tttccgctgg
     3361 caatcgccgc cacctggcgc gcccagccgg cactggctcg gctttgcgta ccgtgctgaa
     3421 cgatgcggcc atacttgcgg gcagcctcca cacacttacg cccctcaaaa acattgtggc
     3481 tgcacggctt ttcgacgtac acatctttcc ccgcctggca ggcccagatc gtcatcaaag
     3541 agtgccagtg attgcaggtg gcaattgaaa tcgcatgcaa gtctttatca tcgagggcct
     3601 tgcgaacatc ctgaacgcat ttcggcgtgt ttccgccgcg ggtctcgacc tgcttaatgc
     3661 gacctgcaaa gaggcggctg tcaggatcga tcagatacgt gacccgaaca ttgggcatcg
     3721 cacagaaggc atcaatgtga ctagacccac ggccacggat tcctgctacc ccaatattga
     3781 tcgtctcatt ggcaccgatg atctgcccgg agcttcgcgt gccggagatg gcgaagctaa
     3841 aggcggtact ggccaaagcc gatgatttaa tgaacgtacg acgactcaaa cgtgccatag
     3901 tcaacctccc ctcgcacgat gcgagttatt tgggaaacag ggaaccaaac ggtcaaacac
     3961 agtttatatt gaacaccatc gtggcgggcc atacggtgag ggacttttca tccgatcctc
     4021 gatgaccccc gtcacgaatc cggtaactca tgcccgctaa gtattgaacc aagcggccat
     4081 ctgtttgtcc agtcacttgt ggcatttcaa agaattattt gccgaatgga gacaacgtcg
     4141 gtgttacctc gaatttggat caagcggctc tgcgacacag tctggtgtgg tgaacgcctc
     4201 tgattctgct tacccactac atggattggg gaaagggccc cgggattgat caagtaacaa
     4261 acgatgggcg cctgtcagtt gcgtcatatc ttcccgaggg ccgtcacggg aaacacagcc
     4321 ggaggatcgt gcgcgtgatg cgtaagctgc gattgatctt ggcaaggcaa gttgagagcg
     4381 ataatctgtt tggcgatatc tagggtaaag tgacactcaa ggaggccttc gctcgatgcg
     4441 gtacaggaac gataaagatg gagagcacaa ctgaggtgaa ccttgcacat gcgcgagaga
     4501 gaaaactgaa aaaatcgcgc gtgacggtga ttgaaatttt ttcaagatgc aatataacac
     4561 tagtgaaagc agtgcatgaa ttggtggcgc gatgaagcat tgcctaagta agaagtgcgt
     4621 ggctgggtag ttgtctgtgt gccagggcgg gaaaggtggc caagtgatgg cttcgatggt
     4681 cgaagccaac ccgccggcac ctattggacg gaggtctgaa ccgtggctga aaagaaggct
     4741 gccaagaaga cggcgaccaa gaaagcggct cccaaggccg ccaaaaaggc cgccaaaacg
     4801 gcaaccaaga aggccgctaa gaagaagtgc tgattgggtt tcagcggctt tcaccgggga
     4861 aatgaaggaa acggacgtcc gactgacccc ggcaggttcc ggaccaagga caacgacgag
     4921 aaagggccgt gatgcaggat ggatcacggc cctagttttt tctcagctca cttcttgggg
     4981 aatcaaacgt cggaatagct cacgagcttc tgcgaatggt tccagggccc ggtctaaaat
     5041 ccccaacgtg taagcaatcg ttacgccata gttggaaatg ggaacctggg cacgacggca
     5101 ccgctcaatg cggctcaata cctcgcggcg attccacatg catgccccgc agtggatgac
     5161 gagccgataa tttgacaagt cctcaggaaa atcgtgtccc tggaccgtgg tgaatcggag
     5221 gggaccacca acatgttgct gtaaccagcg gggaatcttc tcgcgaccga tgtcatccgt
     5281 gatgggatga tggctacagg cctcagccac caacacatga tcaccggtcc gcagggattc
     5341 aatcgccaaa gcgccgcgga cgaattcatt caagtcccct ttctgtcggg ccatcaggat
     5401 agaaaaggat gtcatgctca catcttgggg cgtaacgttg gcgacatgct ggaaggcctg
     5461 actgtccgtc accacaagcg aaggctttcg cggtacttgt tggaagacct gcgccaattc
     5521 tgtatcgcgg acaaccaatg ccatggctcc gtgatcgagg atttcccgca acgtcataac
     5581 ctggggctgg atcagtcgtc ccttgggagc ctctttgtca atcggaatga cgaggacgac
     5641 cagatcgccc ggctcgatca ggtcacgaat cagcggggga ctattgataa agtcctcggg
     5701 tgcggcgcgg atcaaggctt cgcgcacggc ctccaagcct tcccggcgaa gggcggatgt
     5761 gaatacgaag gggatttttt cctgctcgaa ccgccgacat aaagcttcgt tgggcagagc
     5821 gaggtccatc ttattgaaga ccacaatgac tgggaccctg cgttgtcgga attcctggag
     5881 aagctgttcc tcgaaggcct cccagcggcc ggcatcgcaa acaagcaaag ccaagtcggt
     5941 acgatcgaag acctggcgcg tcttctgcac gcgcatttca cctaaagccc cttcgtcatc
     6001 aactcctgcc gtatcgataa aaagcacggg acctaaaggc agcatttcca tgggcttttc
     6061 gacaggatcg gtcgtcgtcc cggccacgtc cgagacgatc gaaacttcct gccgggtgag
     6121 catattgagt aaagcggatt tgcccgcgtt gcgtcggcca aaaatgccaa tgtggagacg
     6181 aaaacttttg ggtgcagggc gataaccggt cacg
//
LOCUS       DAOSUT010000209         6206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5912_length_6206_cov_10.235734, whole genome shotgun sequence.
ACCESSION   DAOSUT010000209 DAOSUT010000000
VERSION     DAOSUT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5912_length_6206_cov_10.235734"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLD05_14245"
     CDS             <1..620
                     /locus_tag="PLD05_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOP78648.1"
                     /translation="DLTVHRLVDAMLLNFKPRINPTELVALGEHCSEREERGEAAERE
                     LTNLKLLSFLSERLGMELDGIVTGVEKYGLFVQGIALPADGFIPVEALSDDYYHFDRA
                     SHSLCGRRSGNQFRLGDLVRVAVARVDLERRQLDFRLLAHQGHPGPNTVAESLTSFGS
                     AERDRKKDKAGKTRHVSLSERVRLQGLRKLQSKKQSAKKKSQKRR"
     gene            696..1751
                     /gene="ltaE"
                     /locus_tag="PLD05_14250"
     CDS             696..1751
                     /gene="ltaE"
                     /locus_tag="PLD05_14250"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOP78649.1"
                     /translation="MTSNKLLDFRSDTVTRPTPGMRAAMAAAEVGDDVLGDDPTVHRL
                     EERVAEILGKEAAVFVPSGTMSNLIAVRVHCRPGDEMICEADAHIYNYEQGGYAQICG
                     VAVRPVQGEWGLIRLEQVEGLVRPNNAHFVRTRLLTLENTHNRGGGRVQPYPLVESLT
                     AWARQSNLRTHLDGARLWNAYVATGISPATWAQHFDTVNVCFSKGLGAPVGSALAGPR
                     DLIAEARRHRKVLGGGMRQAGILAAAALYALDHHIERLAEDHHNAKRLAEGLAHIPFV
                     TVMPIPVETNILLFDIDRAWGSAAAFSAVLKEHGVLINATAPQRLRAVTHLDVTAADV
                     DKALAVLDHVAREHRGK"
     gene            complement(2098..3930)
                     /gene="ilvD"
                     /locus_tag="PLD05_14255"
     CDS             complement(2098..3930)
                     /gene="ilvD"
                     /locus_tag="PLD05_14255"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009103.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOP78650.1"
                     /translation="MRSDQIKLGAARAPHRSLLRATGLKDEDFRKPFIAICNSYTDVI
                     PGHVHLHAVGEFIKECVRAAGGVPFVFNTIGVDDGIAMGHTGMKFSLPSRELIADCVE
                     TMIQAHCFDGMICIPNCDKIIPGMLMAAVRCNIPTIFVSGGPMEAGRTPEGKKVDLID
                     VFIGAAARQDGKLTDEELAELEKLGCPTCGSCSGLFTANSMNCLCEALGIALPTNGTL
                     LATSQERKRLYQRAAYRIVEMVLQFEKLGPGHGLLPREIITRESIDNAMILDMAMGGS
                     TNTVLHMMAVAQEAEVDYDLRRIDELSRRTPNICKVSPSSRYHIEDVHNSGGIHTILG
                     AIQRGRPGLLHLECPTVTGKTLGENILEYDIRNPQVSEEALELAAVTASGQINAPGTS
                     VPRKFRSVRELTPEQLGFDPYDCIREVNNAYNQEGGLAILFGNLAPNGAVVKTAGVLP
                     HMLQFVGPAVIFESETEAYQGIINGKVKAGDVVVIRYEGPRGGPGMQEMLAPTTALKG
                     VKLDDKVALITDGRFSGGTAGACVGHVSPEAAAGGPIGLLQPGDVIEIDIPARKLNVR
                     LSEEALAERRKRWTPPPPRFTRGILARYAKLATSADTGAVLRLD"
     gene            complement(4106..5101)
                     /locus_tag="PLD05_14260"
     CDS             complement(4106..5101)
                     /locus_tag="PLD05_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOP78651.1"
                     /translation="MAKSTFSRRNFLGTTAGVLAMASFRRHEHGFAAPPPTAGDRPVT
                     GAKHPIRLGGPIFGGSSDPEELARQHRERGYRAAYCPGVQINDKERLRAIRDGFKQHD
                     VVIAEVGVWCNLLDPRPDVRAQNMQRMIDGLAVAEEVEARCCVNIAGSFNPDVWFGPH
                     PKNLSEEFFEAAVENARTIIDAVKPKRAKLAYEMMGWSLPDSPDSYLRMIKAVDREAF
                     AVHLDPCNLINCPERFYNNTALLNECFDKLGPWIVSCHAKDLKWEVEMNVHFVEVPLG
                     QGSLDYRTYLQRLATLPADVPLMMEHMKTAEEYQLSQEYLKKVGLEIGVLFEYVG"
     gene            complement(5275..5997)
                     /locus_tag="PLD05_14265"
     CDS             complement(5275..5997)
                     /locus_tag="PLD05_14265"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOP78652.1"
                     /translation="MDSTSFTQQIPALVVTGLGLATAVAPCPLATNIAALSYIGRKVV
                     DRRWVLLSGILYTAGRSFTYFALAAVLVTGLTNLPGLAMTLQYYGHLFLGPILILIGM
                     MLLGLIVIPLPGAKSEQAQALADRYGLIGALLIGVLFALAFCPTSAAYFGAVVTILTG
                     KSAGALWLALLYGVATGLPVFVFAVLIAMGVQWMGKAFQVLTAIDHIVRTATGVIFIV
                     VGIYFILLYDFNLPINIYELLR"
BASE COUNT         1373 a         1826 c         1742 g         1265 t
ORIGIN      
        1 ccgacttgac cgtccatcgc ctggtcgatg cgatgttgtt gaactttaag ccgcggatca
       61 accccaccga gctggtcgcc ctgggcgaac actgctcgga acgcgaagag cgcggcgagg
      121 ccgccgaacg ggaactcacg aatttaaaac tattgtcgtt cctcagcgag cgcctcggga
      181 tggagctgga cggtatcgtg acgggtgtgg agaaatacgg gctatttgtg caggggatcg
      241 ctttacctgc cgacgggttc atccctgtgg aagccctcag cgacgattat tatcattttg
      301 atcgggcaag ccattcccta tgcggacgac gatcgggcaa ccagttccgc ctaggtgact
      361 tggtccgtgt ggccgtggcc cgcgtcgatt tggaacgccg gcaactggat tttcgtctcc
      421 tggcccatca ggggcatccc ggaccgaaca ctgttgccga gtctctcacg agttttggga
      481 gcgcggagcg ggaccgcaaa aaggacaagg caggcaagac acgccatgta tccctttcgg
      541 aacgagtgcg gctccaaggg cttcgcaagt tgcagagtaa gaaacaatcg gctaagaaaa
      601 agtcccaaaa acggcgttga gtaaaaataa tcgtcctcgc gtcacggttt tccactgctt
      661 ggactaaatg aatcattctc gggtagcgac ggatcatgac gagcaacaaa ctcttggatt
      721 tccgtagcga cacggtcaca aggccaaccc cggggatgcg tgccgccatg gccgccgccg
      781 aggtcggcga cgatgtcctc ggtgatgatc ccaccgtgca caggttggaa gagcgtgtgg
      841 cagaaatcct gggaaaagag gctgccgtct tcgtcccctc gggaaccatg tcgaatctca
      901 tcgcggttcg cgtccactgc cgtccgggtg atgagatgat ctgcgaggcc gacgcccaca
      961 tttacaacta cgaacagggt ggctacgccc agatttgcgg cgtggcagtc cgaccggttc
     1021 agggcgagtg gggattaatt cggctagaac aagtggaggg acttgttcga ccaaacaatg
     1081 cccatttcgt tcgcacgcgg cttttgaccc tggaaaatac ccacaaccgg ggtggcgggc
     1141 gtgtccagcc gtaccctctt gtagaatcgc taacggcgtg ggcacgtcag agcaatctgc
     1201 gcacccacct ggacggtgcc cggctctgga atgcctacgt ggcgacggga atcagcccgg
     1261 ccacctgggc ccaacacttc gacaccgtca acgtctgttt cagtaaggga ttgggggcgc
     1321 ccgttggttc ggctttggcc ggcccgcggg atttgatcgc cgaggcccgc cgtcaccgta
     1381 aggtcttggg tggaggaatg cggcaagctg ggatactggc agcggccgct ttgtatgccc
     1441 tcgatcacca catcgagcga cttgccgagg atcaccacaa tgccaaacgt cttgccgagg
     1501 gtctggcaca catccccttc gtcacggtga tgcccatccc cgtggaaacg aacatcctcc
     1561 ttttcgatat tgatcgggca tggggtagtg cggcagcatt ctctgctgtg ctgaaagagc
     1621 atggcgtcct catcaacgcc acggcgccac aacgactccg cgccgttacc catcttgacg
     1681 tgacggcagc ggacgttgac aaggccctcg ccgtacttga ccacgtagcc cgggaacatc
     1741 gcggcaagtg agcgccatcg ccgcaaccac aatagcttga cagacctttg ggggccgtcg
     1801 ccaacctgcg acggagaaag ccgtcaagct ctgtgacgct tcgtgtacgg gtcgcaagct
     1861 ctcaggccga agagtttcct gcgtcgtgac agccggcccc ttttctggtg gctaagagcc
     1921 gatcccataa tctcccctca cccgctcggc gcgagaggag tcggggatga gggtggctcg
     1981 aatcgctgcc ggctttctca atcgattccg acgatcgcct cccctcaccc caaccctctc
     2041 ccagagggag agggggactt tttgggatag actcttattc cgcggcgttc ggggctttca
     2101 gtccaagcgg agaaccgcgc ccgtatcggc actggtcgca agtttggcat agcgggccaa
     2161 gataccccgt gtgaatcgag gtgggggtgg tgtccaccgc tttcgtcgct cggcaagggc
     2221 ctcctcgcta agtcgaacgt tcaattttct cgccggaatg tcaatctcga tcacgtcccc
     2281 gggttggagc agaccaatcg gtcccccggc agcggcctcc ggactgacgt gtcccacaca
     2341 tgccccggcc gttcctccag aaaaccggcc atcggtgatc aaagccacct tgtcatccag
     2401 cttgacgccc ttgagcgcgg tcgtcggcgc cagcatttcc tgcattcccg ggcccccgcg
     2461 tgggccttcg taacgaatca cgacgacatc tccggctttg accttcccgt taatgattcc
     2521 ctggtaggcc tccgtttctg attcaaagat cacagccgga ccgacaaact gcaacatgtg
     2581 cggcaagacg cctgccgttt tcacaaccgc accgttgggg gccaggttac caaacaagat
     2641 cgccagaccg ccttcctgat tgtaggcgtt gttcacctca cggatgcaat cgtaggggtc
     2701 aaaccccaat tgctcgggcg tcaactcgcg gacagatcgg aatttgcggg gcacactcgt
     2761 acctggggcg ttgatttgcc cactggccgt gacggcggcg agttccaagg cctcctcact
     2821 gacttgagga ttgcggatat cgtactcgag gatgttttcc ccgagggtct tcccagtcac
     2881 cgttgggcac tcgaggtgca aaagccctgg tcgtccccgc tgaatggctc caagaatcgt
     2941 gtgaatcccg ccggagttgt gcacatcctc aatatggtat cgactgctcg gggacacctt
     3001 gcagatattg ggggtcctgc ggctcaactc gtcgatccgc cgcaggtcgt agtcgacctc
     3061 tgcctcctgg gccacggcca tcatgtgcag gaccgtattc gtgctaccgc ccatggccat
     3121 atccagaatc atggcattgt cgatggattc acgagtaatg atctctcgtg gaaggagccc
     3181 atgaccaggg ccgagctttt caaactggag gaccatttcc acgatccgat aggcggctcg
     3241 ctggtagagg cgcttccgct cttggctggt ggccaacagg gtaccattgg tgggcaaggc
     3301 gataccgagg gcttcgcaaa gacagttcat actgttggcg gtgaataacc cagagcacga
     3361 cccacaggta ggacagccca gcttttccag ctcggccaac tcttcgtccg tcaatttgcc
     3421 atcttgtcgg gcagccgcgc cgataaagac gtcaatcaag tccaccttct ttccctcggg
     3481 cgttcgaccg gcctccatgg gaccgccgct gacgaagatc gtcggaatgt tgcaacgcac
     3541 ggcggccatc aacatgccgg ggatgatctt gtcacaattg gggatgcaaa tcatgccatc
     3601 gaagcaatgg gcctggatca tcgtctccac gcagtcggcg atgagttccc ggctggggag
     3661 cgaaaacttc atgccggtgt gccccatcgc aatgccgtcg tccaccccaa tggtgttgaa
     3721 gacaaaagga acgcccccgg cagctcgaac gcactcctta atgaattccc ccacggcgtg
     3781 gagatgaacg tgaccgggaa tgacgtcggt ataactgttg caaatggcaa taaatggctt
     3841 gcgaaaatcc tcatctttca ggcccgtcgc gcgcaaaaga ctgcgatgag gtgccctcgc
     3901 agctcccagc ttgatttgat cggaacgcat tatctctccc tcgctcgatg gacagaacaa
     3961 cgtcaatcgt gcctttttta aatagcccaa caaacgggct actgcaaaga ccaaacgctt
     4021 tattctattc ctccccaaca caatcaacca gccgcggcat tttggaggac aatcaccaac
     4081 gccggtgggg accggtattt cgttgtcatc ctacgtactc aaaaagcacg ccgatctcca
     4141 ggcccacctt tttcaaatac tcctgcgaaa gttggtattc ttctgcagtc ttcatgtgtt
     4201 ccatcatgag ggggacgtct gcgggcaagg tcgccagccg ctgcaaatac gtgcggtaat
     4261 ccagggaacc ctgtcccagt gggacttcga caaagtggac gttcatctcg acttcccact
     4321 taaggtcttt ggcatgacag ctcacaatcc acgggcctag tttgtcgaag cactcattga
     4381 gcaaggccgt gttgttgtag aaccgctcag gacaattgat gagattgcac gggtctaaat
     4441 ggacggcaaa ggcctcgcga tccaccgcct tgatcattcg caggtaacta tccgggctat
     4501 ccggcaatga ccagcccatc atttcatagg ccagcttagc ccgtttgggt ttgaccgcgt
     4561 cgatgatggt gcgggcattc tctacagcgg cttcgaaaaa ctcttccgag aggttcttcg
     4621 gatggggacc aaaccagaca tcggggttga acgagccggc gatgttgacg caacagcggg
     4681 cctcgacttc ttcggcaacg gccaatccgt caatcatgcg ctgcatattc tgtgcccgca
     4741 cgtctggtcg cggatcgagc aagttgcacc agaccccgac ctcggcgatc acgacatcat
     4801 gttgtttgaa accatcgcgg atcgcccgca ggcgctcttt atcgttgatc tgtacacctg
     4861 gacagtatgc ggcccgatac ccccgttccc gatgctgccg ggccagttcc tcggggtccg
     4921 acgagccgcc aaagatcggg ccaccgagac gaatcggatg tttcgcaccg gtcactggtc
     4981 gatcgccggc cgttgggggt ggagccgcaa aaccatgttc atgccggcga aaactggcca
     5041 ttgctaggac tcccgctgta gttcccagga aattacgccg agagaatgtt gattttgcca
     5101 tggtagttgg ctccgaaatc gtgagaccca aatgattcag aagcgggcaa gggaaagacg
     5161 atgctcctcc ccgaggtcgg aggtcgcctc tattgtagca agaagaggac gctgaggaag
     5221 cagatcaaac cgcaacccgg cagtatgagg aagatgaacg agtctgtcgg ggccttaacg
     5281 caacaattcg taaatgttga tcggcaagtt gaagtcatac agcaaaatga agtaaatccc
     5341 aaccacgatg aagatgacgc ccgtcgccgt gcggacaatg tggtcaatcg cagtcaggac
     5401 ctggaaggcc ttacccatcc attgcacacc catggcgatc aacaccgcaa acacgaagac
     5461 cggcagccct gtagcaacgc cgtacaacag ggctagccag agggcgcccg cggatttgcc
     5521 ggtgaggatt gtcacgacag ctccaaaata cgctgctgac gtgggacaaa acgctagggc
     5581 gaacaaaaca ccgatcaaca acgcgccgat caaaccataa cgatcggcca aggcctgcgc
     5641 ttgctccgat ttggcccccg gcaagggaat gacaatcaat cctaagagca tcatcccaat
     5701 gaggatcagg atcgggccaa ggaaaagatg accatagtac tgaagggtca ttgccaaccc
     5761 tggaaggttg gtcagtcctg ttactaggac agcagctagt gcaaaatagg taaacgatcg
     5821 cccggccgtg tagagaatgc cggaaagaag gacccagcga cgatccacca cttttcggcc
     5881 aatgtacgat aaggcagcga tattggtggc aaggggacag ggcgccaccg cagtggccag
     5941 cccaagcccc gtgacaacga gggccggtat ttgctgtgtg aagctcgtgg agtccatcgt
     6001 cggctatttc cctaattcac tcaagataga tgttcaaaag ggtcgcaaga ccacactcag
     6061 cggaccacac tggcctgtgc gtgccctaac tgatgaaggt aagcctcaac gttgctggtt
     6121 acgtactgat ccaattcttg cggtttgatg agcaaactcc acaccttcgg catgggctgc
     6181 cacgccacca cgcgaccacc ttcgac
//
LOCUS       DAOSUT010000210         6193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5924_length_6193_cov_9.363636, whole genome shotgun sequence.
ACCESSION   DAOSUT010000210 DAOSUT010000000
VERSION     DAOSUT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_6193_cov_9.363636"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(777..849)
                     /locus_tag="PLD05_14270"
     tRNA            complement(777..849)
                     /locus_tag="PLD05_14270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Ala,seq:cgc)
     gene            complement(1626..2207)
                     /locus_tag="PLD05_14275"
     CDS             complement(1626..2207)
                     /locus_tag="PLD05_14275"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOP78653.1"
                     /translation="MIENNPSNVSAAFEMLLEEIEAEVDFVNRIGARAFEGREYDKAR
                     EALERVAALTAFRDKVAALRKEWDAMAAVADRAEDEETRSQRRNMGRLPKGVRTPEKA
                     FYRPILQALQEFGGTAEIGKVLERVEELMRPVLKDVDYEPLASDADLPRWRNTAQWAR
                     NSMVKEGLLKADSPRGIWAISDLGRKYLAESGG"
     gene            complement(2222..5839)
                     /locus_tag="PLD05_14280"
     CDS             complement(2222..5839)
                     /locus_tag="PLD05_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOP78654.1"
                     /translation="MNDLFPKIQSQLAHKLSDNLETLFRGTLKWGEARRMPPRKLQLG
                     VPVNKTLTAQPVAQLSGVCVYRIDWPEDRLPGITARRAVQRALSALHAEYLVCYVTQN
                     KKQAAFVWAHRRPDGKTELRTLPYEVGSPARTTIERLAEIAFTRDEFPRTGEPPITAV
                     TDKLSRAFSVEAVTSEFFEAYKTVFADLQQRLYRLSDDKVWSHDYALQLLNRLMFLYF
                     IQRKQWLGGDPRFLANFWRAYKTSRQPKDTFFDQWLSVLFFEAFNNKFHGGHRQFPDH
                     IRSALAQAPYLNGGLFTRNSLDNAYHIKVPDEFFATLFDRFNGSTPGFLERYNFTITE
                     STPLDIEVAVDPEMIGKVYESLVNITFEGLTEEDQRGSAGIFYTPRVEIDLMCRLSLV
                     DALANKLGESRKPMLYDAVFAYDAIDKDKADQALAAQNLWPELNRLLREITVCDPACG
                     SGSFLVGMLLVLDDLQARANAQLGIEETPYERRRRIIGEQLYGVDVMDWAVHVAELRL
                     WLQLVVETELKPAELKFRPLLPNLSFKIRCGDSLVQEIAGVNFGLHYSDPDVPAPLKG
                     RITQLKGKKLRFYQGETNLSETELKAEELAIFRDILKHKRDALQQEIDRLTRQIESRA
                     EQGEFAAFSTGRPKQERLNFQKQWRAERDEKQAELKRIEKNLEALCPTKPVPFVWDIA
                     FVEIFKGDKHGFDIVIGNPPYVRQEMIAPPTLDAADFGGENSDRWKEQKKTYKTKLQQ
                     SVAAAWPKFFGYTPPSDKFRKVDGKSDLYVYFYLHGLSLLNSQGSFCFITSNSWLDVG
                     YGADLQEFLLKHSHIKFILDNERKRSFASADVNTIIALLARPDDKDDTGLDKTARFVM
                     FKVPFEDVLNAETFKALEAATERQSTDKWRICVQSQRELYEEGLVHEEDETEPIAGKI
                     KTLHIPRRYEANKWGGKYLRAPEIFFTILEKGKGKLVRLGDIAEVRRGFTTGANEFFY
                     LDEEKIRQWGIEEEFLKPVIKSPRECRSIVIKPEDLKFKIFMCHKEKSELRGTNALEY
                     IKWGESEGFHKRPSCRGRQRWWDLGDRRAPPIVSPSSISGFYRAFLNDARVLIDKRLY
                     EVIPNAGGARELAVSLNCTLTTLFLELGSRIGLGEGLLDMTVYEVANCLTLGPFLVKD
                     VPLFNRPVQEVECEIRQPDRRALDDIVFDVLGLTAGEREAAYEAVVNLVRARLEKARS
                     V"
     gene            complement(5832..>6193)
                     /locus_tag="PLD05_14285"
     CDS             complement(5832..>6193)
                     /locus_tag="PLD05_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78655.1"
                     /translation="FGLLGPLRGVRRKLYERLDRYRKLQQEHPTSSSDEILERLDTLI
                     DLIYRYPLKRAAQDPISRQIRLGITDDALLELVWRHAEDKTLCEITEEDEASLSEPQI
                     ICSIGLVNDQWGENSHE"
BASE COUNT         1299 a         1671 c         1784 g         1439 t
ORIGIN      
        1 gagttgtggc ttgagtggaa cgaagcctac caaacattga cgcagcggat gtacgaggaa
       61 aagaccaatt ttcaccaact cgagatgatg gccgaccaac ttgacgcctt acgtctgaag
      121 gctgtggagg aaacgcgcag actgctggtt gcgttgcaac cttgccagta gcgttggagc
      181 tctaatcctt gaatcggatc accgtgctcg gtctgaagac atccaaccaa aactagccgt
      241 accgggacat tccgcagttc ccagtgatga gcccttacct ttgaggacct tcggcagatt
      301 atccctccgg tggagggtac gtttacccaa acgattggca gaaacgaacc gaacgcccga
      361 aggcctgggc caatgcaaac tttcgaacga ctttcaacac gatcatcaag cgggctgtga
      421 aagcttgtcg caagctctta tacaaccttc gcgagcgtct tggtagcgaa tctcccccta
      481 acgatggcga cgaagtggtt ttcccacggg cctagtaccg cgccttgtcg gtaaattgat
      541 ctccgaggaa agacttttga tgtgaccccc attttggaca caactgggtt agccgcgcga
      601 ggcatcccgg ggagcgaccc accccagatc aaattcgaag ccggcctggc gtcaaaaccg
      661 acatcgccaa atgcagcgaa actgacgaca ttcacctgcc tggccgcaag cgacgtcaag
      721 atgcaccggt gaacttttat ctgagattag ttatagtttg atgcaaatgc caaaattgga
      781 gcggagggga cttgaacccc cgacctctag agtgcgattc tagcgctctc ccaactgagc
      841 taccgcccca gagtatcttc tggtagttat tccccgcgac tccccacatt tatgaggata
      901 gcactttgct cgcttccttg ccttgcatac cgtattatcg agcagcccgc gaaagaacca
      961 gattccagct ttgggcccgc ggacacccaa cctcccaacg aaaaatgggc acgcgggact
     1021 cgaacggatt tccgcaaatg gcgatttaac cgcaatttac gacgatacca aaaaatcctt
     1081 ggccatgcca gcggccatac tgccgaccaa aacaatccca gacccgtccg acttagcttc
     1141 cttggtggat atcatccgtg cgctacctgt cgaggcccgg gagcaactac gccggctttt
     1201 cgaccaatta taatggtcca tgcgggcttg tctagggcgg tgaagggcga gaaggctagg
     1261 acaacgaatt tgtttcacct caaatgggcc gacatcatgt tccctcttcg cgcccatggg
     1321 ggctagtcca tgtggattag cttcctgaca aagcttctac cgcccacaaa caccacgaag
     1381 ccaccaaccc tgaatcttgg acgggcattt gtggcgccct tgggacgggt ctgttacccc
     1441 acatcagtct gggtcaagag ttgtccaacc taaaggagga cgcggtatcg ggcaggcacg
     1501 ggggcctgcc cctacgaatt cgatgcgatg tgtaggggca accccctgtg gttgcccgga
     1561 tcgtgacaac gccggggcag cccccaatct cgtgttaatc ctgcattgaa gcaccgcgtc
     1621 ggccgttaac cgccactctc cgccaaatac ttccgcccca agtccgagat ggcccagatt
     1681 ccccgcggcg aatcggcttt aagaagccct tccttgacca tactgttgcg tgcccattgg
     1741 gcggtgttcc gccaccgagg caggtcggca tcacttgcga gaggctcgta gtccacatcc
     1801 ttcaaaaccg gtctcatgag ctcttccact ctttccaata cctttccgat ctcagcggtg
     1861 ccgccgaact cttgcaacgc ctggaggatg ggacgataaa aggccttttc gggcgtgcgg
     1921 acgcccttgg gcaggcggcc catattgcgg cgctgcgaac gggtttcctc gtcctcggcg
     1981 cggtcggcta ccgcagccat cgcatcccat tccttgcgca atgctgcaac cttgtcgcgg
     2041 aaggcggtca acgccgcgac ccgctccaaa gcctcgcggg ccttgtcgta ctcgcgccct
     2101 tcgaaggcac gtgcgccaat acggtttacg aaatcaactt ccgcttcgat ttcctccaaa
     2161 agcatctcga aggccgctga cacattgctc ggattgtttt caatcatggc ctgctccttt
     2221 ctcacacact tctggccttt tccaggcggg cgcggacgag gttgaccacc gcctcatacg
     2281 ccgcctcccg ctctcccgcc gtcagcccca acacgtcaaa aacgatgtca tctaaggccc
     2341 gtcggtcggg ttgacgaatc tcacattcga cctcttgcac gggtctatta aataaaggca
     2401 cgtcctttac gagaaatggc cctagtgtta aacaattcgc aacctcgtaa accgtcatgt
     2461 ccaatagccc ctcgcccaag cctattcgag aacccagctc aagaaacaag gtcgttaagg
     2521 tgcagttcaa gcttacggca agttccctag cgcctccggc gtttggaatg acctcgtaca
     2581 ggcgtttgtc tataagcacc cgggcatcat tcagaaacgc gcgataaaaa ccgctgatgc
     2641 tactcggaga tacaattggc ggcgctctcc tatcccctaa atcccaccac ctctgccttc
     2701 ctctgcagct tggtcgctta tgaaatccct ctgattcgcc ccacttgatg tattcgaggg
     2761 cgtttgtgcc gcgcagctcg cttttctctt tgtggcacat gaagattttg aatttcaggt
     2821 cctcgggttt aataacgatg gagcggcact cgcgcgggct tttgatcact ggcttgagaa
     2881 actcttcttc gatcccccat tgccgaattt tttcttcgtc caaatagaag aactcattgg
     2941 cgcccgtcgt gaagccgcgg cgcacttccg cgatgtcgcc gagccggacg agtttgcctt
     3001 tgcccttttc cagaatggtg aaaaagattt cgggggcacg aaggtattta ccgccccatt
     3061 tgttggcctc gtatcggcgt gggatgtgga gcgttttgat ctttccggcg ataggttctg
     3121 tttcgtcttc ttcgtggacc aggccttctt cgtagagctc ccgctgggat tgcacgcaga
     3181 tacgccattt gtctgtgctt tgcctttcgg tggccgcttc gagggccttg aatgtttcgg
     3241 cgttgagcac gtcttcgaaa ggaaccttga acatgacgaa tcgggcggtc ttgtcgaggc
     3301 cagtatcgtc tttgtcgtcg ggccgggcga gcaaggcgat gatcgtgttg acgtcggccg
     3361 aggcgaacga acgtttgcgc tcgttatcga gaatgaattt gatgtgactg tgcttgagca
     3421 aaaattcttg caaatcggcg ccgtagccca catcgagcca actgttggag gtaatgaaac
     3481 agaagctgcc ctgcgaattc agaagggaca acccgtgtaa atagaaatag acatagaggt
     3541 cgctcttgcc gtcaacctta cggaacttgt cgcttggggg cgtgtagccg aagaatttcg
     3601 gccaggcggc ggcaacggat tgctggagct tggttttgta ggttttcttc tgctctttcc
     3661 agcggtccga gttttccccg ccgaagtcgg cagcgtccag tgtgggtggg gcgatcattt
     3721 cttgccggac gtaaggcgga ttgccgatga cgatgtcaaa gccgtgctta tcgcctttaa
     3781 aaatttcgac gaaggcaata tcccagacga aaggcacggg cttggttggg caaagggcct
     3841 cgaggttttt ttcgatgcgc ttgagttcgg cttgcttttc gtcgcgttct gcccgccatt
     3901 gtttttggaa gttgagccgt tcctgtttgg gtctgccggt ggagaaagca gcaaattcac
     3961 cctgttcggc gcgagattcg atctggcggg taagcctgtc gatctcctgt tgtagggcgt
     4021 cgcgtttgtg cttgaggatg tcgcggaaga tagcgagttc ctcggccttg agctcggttt
     4081 cggacaagtt ggtctcgccc tggtagaagc ggagtttttt gcccttaagt tgggtaatgc
     4141 ggcctttgag tggggcgggg acatccggat cggagtagtg taggccgaaa ttgacgccgg
     4201 caatttcttg cacaaggctg tcgccgcagc ggattttgaa agaaagattg gggaggagcg
     4261 gtctaaattt gagttcggcg ggcttcagtt cggtttcgac gaccaattgc aaccagaggc
     4321 gtagttcggc aacgtggacc gcccaatcca taacgtccac gccatagagt tgctcgccga
     4381 tgatgcgacg gcggcgttcg taaggcgttt cctcgatacc aagttgggcg ttggcccggg
     4441 cctgaagatc gtcgagcacc agcaacatgc ccaccaggaa cgagccactg ccacaggcag
     4501 ggtcgcagac ggtaatttcc ctcaacaggc ggttaagctc cggccaaagg ttctgagcgg
     4561 caagggcctg gtcggccttg tctttgtcga tggcatcgta ggcaaagacc gcgtcgtaga
     4621 gcatgggttt gcgggactcg ccgagtttgt tggccagagc gtccacgagg ctgagacggc
     4681 acatcaggtc aatttccacg cgcggcgtgt aaaagatgcc ggcggaaccg cgctgatctt
     4741 cttcggtgag gccctcgaag gtgatgttga cgaggctttc gtaaaccttc ccgatcatct
     4801 cgggatcaac ggccacctcg atatccaggg gtgtggattc cgtaatggtg aagttgtagc
     4861 gctcgaggaa gccgggcgtg ctgccattga agcggtcgaa cagcgtggcg aagaactcgt
     4921 cgggcacttt gatgtggtag gcattgtcga gactattgcg ggtgaacaag ccaccgttaa
     4981 gatagggggc ttgggcaaga gcagagcgga tgtggtcggg aaattggcga tggccgccgt
     5041 ggaatttgtt gttgaatgcc tcgaagaaca ggacggaaag ccactggtca aaaaaggtgt
     5101 ctttgggctg acgggaggtc ttgtaggctc gccagaaatt ggcgagaaag cgggggtcgc
     5161 cgccgagcca ctgtttgcgc tggatgaagt aaagaaacat cagacggttg aggagctgga
     5221 gggcgtagtc gtgcgaccag accttgtcgt ccgaaagccg gtaaagtctt tgttgcaagt
     5281 cggcaaaaac tgttttgtag gcttcaaaga actcgcttgt gacggcttcg acgctgaagg
     5341 ccctactaag cttgtcggtg acggcggtga tgggcggctc accggtgcgg ggaaattcat
     5401 cccgtgtgaa tgctatttcg gcgaggcgtt cgatggtagt gcgggctggc gaaccgactt
     5461 cgtagggaag tgtgcggagc tcagttttgc cgtctggccg gcggtgtgcc caaacaaatg
     5521 ccgcttgctt tttgttctgc gtcacgtagc agactaggta ctcagcatgc agggctgaaa
     5581 gtgcgcgctg aacggcccga cgggcggtga tgcccgggag gcggtcctct ggccagtcta
     5641 tgcgatagac gcaaactcca gaaagctgcg ccaccggctg ggccgtgagg gttttgttca
     5701 ccggtacacc gagttgaagt ttacgtggcg gcatacgtct cgcctcaccc cacttgagcg
     5761 tcccgcgaaa gagcgtctcg aggttgtcgg aaagtttgtg ggctagttgc gactggattt
     5821 tcggaaacag gtcattcatg gctgttttct ccccattggt cattcacaag gcctatcgag
     5881 caaataatct gtggctcgga gagcgaggct tcgtcttctt cggtgatttc acaaagggtt
     5941 ttatcttcag catgtcgcca gacgagttct agcagggcgt cgtcggtgat gccgagacgg
     6001 atttgacggc taatggggtc ctgggcggcg cgcttgagcg gatagcggta gatgaggtct
     6061 attaaagtgt cgagtcgttc gaggatttca tcgctggaag aggtggggtg ttcctgctga
     6121 agcttccgat agcggtcgag ccgttcgtag agtttcctac ggacgccacg caatggaccc
     6181 aggagaccga agg
//
LOCUS       DAOSUT010000211         6155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_5962_length_6155_cov_8.851148, whole genome shotgun sequence.
ACCESSION   DAOSUT010000211 DAOSUT010000000
VERSION     DAOSUT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_6155_cov_8.851148"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1547)
                     /locus_tag="PLD05_14290"
     CDS             complement(240..1547)
                     /locus_tag="PLD05_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78656.1"
                     /translation="MSDVLKTSRRSFLETSAVVGGAAMLGSLSLARSVHAAGSDVMKV
                     CLVGCGGRGNGAVRDHIRAASEAGYKVQVVAVADAFEDRAKGAADSLRKEFGKDIVDL
                     PDDRVFVGLDSYKKAIDCGVDIVINASPPGFRPFQFAYAIKAGKHVFMEKPVCVDAPG
                     FRMVMEACKLADQKGLKVGVGLQRHHQASYIREIAAIKEGKIGDIMFLRAYWNGGAIW
                     IRRRREDQNELQYQVYNWYHFTWLSGDNIVEQHVHNLDVCNWVMSDDLTNPVHPVEAN
                     GMGGCEMRYRGENRGVGQIYDHHFVEFTYPNGVKMYSQCRQINNCWNNVSEAVHGTKG
                     VSGCQSGPGGNNPYVQEHIDLLNAIAKNERFNEGYFGAVSSFTAVLGRMATYSGQVVR
                     WDEAVEKGPMIAPEITSWDQEPPVKKDENGDYPIAQPGVYKPY"
     gene            complement(1703..2842)
                     /locus_tag="PLD05_14295"
     CDS             complement(1703..2842)
                     /locus_tag="PLD05_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOP78657.1"
                     /translation="MSRKGWSHWLILGLVVGSLIAAYGWAQAEDAASNKPDPALEPVI
                     KWLPEPYPVPDADAKTEAEMKPYTEVIVGTDIKFDMVPIPGGRFIMGSPENEKGRKPD
                     EGPQHEVEIEPFWMAKYETLWDAYETWSFELDKRRREQTKEPATKWDEIADAIARPTP
                     PYTDMSFGMGKKDRPAVCMTLYAAQHFCKWLSAKTGRYYRLPTEAEWEYACRAGTKTA
                     YSFGDNVDDLEDYAWFFDNADDKYHKVGEKKPNPWGLYDMHGNVAEWVLDQYVPDAYK
                     QFAGKVVKNPLIPATKEFPIVVRGGSWMDDPEMLRSAARRGSHPDWKMQDPQIPQSIW
                     YLTDAPFVGFRVVRPLRLPTEEEAKLYEPDPEILLNYRKAQGGKQ"
     gene            3425..4546
                     /locus_tag="PLD05_14300"
     CDS             3425..4546
                     /locus_tag="PLD05_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOP78658.1"
                     /translation="MNQYWLGSTIIVFGFCVGGWSIGISSAVGHESPIACQADAPLCS
                     PSPQAQAKRWTWEGVEMAAPLRIVCYHSDERQAARAMQKAVERIRELNNVFSDYDASS
                     EVRRLCDTAGSGRPIPVSEDLWRLLELSLRISQETDGAFDVTVGPLSRLWRRARMSKE
                     MPPAWRFDEARKAVGYHLVKMNPEKRTVELLRPGMRLDFGAVAKGYAIDAALAVLRDH
                     GVASALVDLGGDIGLGDPPPDRGPWKVAIAPLKDDQSPSFFVHGSRCGIATSGDRYRF
                     VVIDGRRYSHIVDPRTGIGLTEQCEVTVIAPNATLADILATAVTVLGAEKGLAWIDTL
                     PDTAALYLRLRDEKTEVLTSAHWSRLAGDSYKVPADMNP"
     gene            4745..>6155
                     /locus_tag="PLD05_14305"
     CDS             4745..>6155
                     /locus_tag="PLD05_14305"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOP78659.1"
                     /translation="MKASIDRSINFGWTLLWMAVLLGPAGLNAAEQASPSCDSWPFAI
                     TAAHRPGTYFWCPGSAFTREDIDWNLRQLHDAGFGFVHVIPIYGARGAESQYIPYLAP
                     QWMEMLDYLVRRAESLGMFVDMTTGTGWCFGGPNLPDWAIDARAQYDAASGAVTLVPA
                     MQVKRAAPGGEGPMLNPFSPRAMDLYLERFSAAFDSSRVARPRAQYHDSFEYQSNWSA
                     DLFEEFRSCRGYDLKEHLSLFFDESRSADEERTRLKCDYRWTLAELHLELIRRWVAWA
                     HQHGMLTRNQAHGAPGNLLDLYAASDIPETEMFGAPELPIPGFRRDPAMVRAADDDPR
                     ICLLASSAAHVAHPRGQQLVSAETCTWLREHWHTSLAQIKLQLDLFFLTGVNQLLFHG
                     TCYSPKDAPWPGWFFYASIKMDWRNAFWRDVPVLTQYVARCQSVLQAGQHDNDVLLYW
                     PIFDLWMDPKGMTIPLTVHR"
BASE COUNT         1257 a         1809 c         1697 g         1392 t
ORIGIN      
        1 ggagaagtat cggcaaatcc tgcgggtcgt ccgtcgcgcg gcataatcac ggcagattac
       61 cccgcgctca cttcaaaaca ctcaacgacg gtcttcgcca ggattaagtg tgcaaaaagc
      121 ataaaaaaag cggccaagac gcaacgtctc agccgccaat gcccactagc gacagggctg
      181 cgtgagggga aatccccgtc gcttacaccc caaagaccaa agctatcaac gaaggggact
      241 cagtacggct tatacacacc gggctgggcg atcgggtaat cgccgttctc gtccttcttg
      301 acgggcggct cttgatccca ggaggtgatt tccggcgcaa tcattgggcc tttttcaacc
      361 gcctcgtccc agcggaccac ctggccgcta taggtcgcca ttcggccgag gaccgccgtg
      421 aagctgctga cggcgccgaa gtacccctcg ttaaagcgtt cgttcttggc aatcgcgttg
      481 agtaagtcaa tgtgttcctg gacatacggg ttgttaccgc cggggccgct ttggcaccca
      541 gagacaccct tcgtgccgtg gactgcctcg gacacgttgt tccagcaatt gttaatctgc
      601 cggcactggc tgtacatctt gacgccattg ggataagtga attccacgaa gtggtggtcg
      661 tagatttggc caacgccgcg attctcgccg cgatagcgca tctcgcagcc acccatcccg
      721 ttggcctcga cggggtgcac gggatttgtc aaatcatcgc tcatcaccca gttgcaaacg
      781 tcgaggttgt gcacgtgctg ctcaacaatg ttatcaccgc tcaaccaggt gaagtgatac
      841 cagttataca cctggtattg aagctcattt tgatcttccc gacggcgtcg aatccaaatc
      901 gcaccaccgt tccaatatgc ccggaggaac atgatatcgc cgatcttgcc ttccttaatg
      961 gcagcgattt cgcggatgta ggaggcctga tggtgccgct gcaagccgac gccgaccttc
     1021 aagcccttct gatccgccaa cttgcacgcc tccatgacca tccggaaacc cggggcatcg
     1081 acacaaacgg gtttttccat gaagacgtgt ttgcccgcct tgatggcata agcgaactgg
     1141 aagggacgga agcccggcgg cgaggcattg atgacaatat caacgccgca atcaatggct
     1201 ttcttatatg aatctaagcc gacaaagact cgatcatccg gcaaatcgac aatatctttc
     1261 ccgaattcct tacgaaggct gtcggctgca cctttggcac gatcttcaaa ggcatcggcc
     1321 acagccacaa cctgcacctt gtagcctgct tcactggcgg ctctgatgtg gtcacggacc
     1381 gcgccatttc cccggccacc gcaacccacc aagcaaacct tcatgacatc gctgccggcg
     1441 gcgtgaaccg acctggccag actgagcgaa cccaacatag cggccccacc aacgaccgct
     1501 gatgtctcca agaaacttct ccgagatgtc tttagcacat cactcatgac gcaggctcct
     1561 tacaaaaagt tctagcaaaa acccaagttg ttcttaccgc cactgtcgtc tttgaccgac
     1621 gaggcagctc gacttctcaa gacatacaga ttatcccctt cgcacgtcgt tcgcaactga
     1681 tgcgggagcc agcgacttca cgtcactgct ttccgccctg cgccttgcga tagttcagaa
     1741 ggatttccgg atcgggttca tacagtttgg cttcctcttc ggtgggcaga cgtaatggcc
     1801 gaaccacacg aaaaccgacg aacggggcat ccgtcagata ccagatactt tgtggaatct
     1861 gggggtcctg cattttccaa tcaggatgcg aacctcggcg ggcggcgctc cgcaacattt
     1921 cagggtcgtc catccaggac ccgccccgca cgacgatggg gaattccttc gttgccggga
     1981 tcagaggatt cttcaccacc ttgccggcaa actgtttgta ggcgtccggc acatattgat
     2041 cgaggaccca ttccgcgaca ttgccgtgca tgtcataaag cccccaagga ttgggcttct
     2101 tttccccaac cttgtgatac ttgtcgtcgg cgttatcgaa gaaccaggca taatcttcga
     2161 ggtcatccac gttgtcgccg aaggaataag ccgtcttggt acctgcgcga caggcgtatt
     2221 cccattcggc ctccgtgggc aagcggtaat atcggcccgt cttggcggag agccacttgc
     2281 agaaatgctg ggccgcgtac aacgtcatgc aaacggcggg gcggtccttc ttgcccatcc
     2341 caaaggacat gtcagtataa ggcggcgtgg gacgggcaat ggcgtctgcg atttcatccc
     2401 acttcgtagc cggttctttg gtttgctccc gacggcgctt atccaattcg aaggaccatg
     2461 tttcataggc atcccacagc gtttcatatt tggccatcca gaaaggctca atctcaacct
     2521 catgctgagg cccttcgtcg ggcttacgtc ctttttcatt ctccgggctg cccatgatga
     2581 acctgccacc ggggatcggg accatgtcga acttgatgtc ggttcccacg atgacctcgg
     2641 tgtaaggctt catctcggct tcggtcttgg cgtcggcgtc ggggacagga tacggctcgg
     2701 gaagccactt gatcacgggc tccagcgccg gatcaggctt gttggaggct gcgtcctcgg
     2761 cttgcgccca cccatacgcc gcaatcaacg accctaccac caatcccaga attaaccagt
     2821 gactccaacc ttttcgactc atgcaacgtc acctcattga tcaattgggt tcaagggaca
     2881 aaccgaccac tatcgccttt gcaatctagc cagcatcctc taggggagta actcaaagct
     2941 catttctcgc tttgtgcccc gttgtcaaag cctggttaaa tgcctcactt atgatgcccc
     3001 gggagactcc tgcccgaccc gccaaccttt ccctgattgc aactccccat gataataact
     3061 ggtattgtaa actttgttcc ccagggacgc aaacgggttg gaatctccgg aaactgcccc
     3121 gctctcctcg caaaggcgtg aggtcatcag aacggcaccc ggcatgtgtc ggccctaggg
     3181 ggaataagag tctatcccaa aaagtccccc tctccctccg ggagagggtt ggggtgaggg
     3241 caatcgaacg tcagaatcga atcgattgag aaaaccggca gcgattcaag ccaccctcac
     3301 ccccgccccc tctcccgccc aacgggtgag gggagatcat gggataggct ctaagtatgg
     3361 gtcatgcctc cgccccgttg gacagccttt cttacgatct gaccaccaat tgaggtcttg
     3421 caaaatgaat caatattggc ttggcagcac gattattgtt tttgggtttt gtgttggagg
     3481 ttggtcgatc gggatttcct cggcggttgg ccatgagagc ccgatcgctt gccaagcgga
     3541 tgccccgctc tgctcgccgt caccgcaagc gcaggcgaag cggtggacct gggaaggcgt
     3601 ggaaatggca gccccccttc ggattgtgtg ctatcacagc gacgaacggc aagccgcccg
     3661 ggccatgcaa aaggccgtcg agcgaattcg tgagcttaac aacgttttta gcgattacga
     3721 cgccagtagt gaggtccggc gactgtgcga cacggccggg tctggaaggc ccatccccgt
     3781 cagtgaagac ctctggcggc ttctggagtt gagcctccgc atttctcagg aaacagacgg
     3841 agcctttgac gtaacggtgg gcccgctcag tcggctttgg cgacgtgctc gcatgtccaa
     3901 ggaaatgccg ccggcctggc gctttgacga ggcgagaaag gctgtgggtt atcacctggt
     3961 gaaaatgaac ccggagaagc ggactgtcga attgcttcga cccggaatgc ggttggattt
     4021 cggcgctgtg gccaaggggt atgctatcga tgcggccttg gcagtcctgc gagatcacgg
     4081 cgtggcatcg gccttggtcg atttgggagg ggacatcggc ctgggcgatc ccccaccgga
     4141 tcgaggccct tggaaggtcg ccatcgcccc gctcaaagat gatcaatcac ccagcttttt
     4201 tgtccatggg agccgttgcg gtattgccac gtcgggtgat cggtatcggt ttgtcgtcat
     4261 cgacggccgt cgttattcgc atattgtcga tccgcgtacc gggatcggac ttactgagca
     4321 gtgtgaagtg accgtgatcg cgccgaatgc aacgctggcc gacatcttgg ccacggccgt
     4381 taccgttttg ggagcggaaa agggactggc ctggatcgac acattgcccg acacggcggc
     4441 cctctatctc cgccttaggg acgaaaaaac agaggtgttg acgtcggccc attggtcgcg
     4501 attggccggc gattcttaca aagtccccgc ggacatgaat ccttgatctc atccagatga
     4561 tgggcatccg gggtccagcg tcgggattta gcttgcttgt tgcatcgtag ggttgctatg
     4621 cgagaaactc ggcgggcctt atgatgagat tcgctctttc acgagcagcg tgtttggtag
     4681 agatttccca tacgaccttg attacccgtt gtcttgccgc gatggcgacc ctacggaggc
     4741 tgaaatgaag gcgtctattg atcgatcaat caactttggg tggacccttc tctggatggc
     4801 ggtgttactg ggcccggccg gactcaacgc tgccgagcag gcttcgccct cgtgtgactc
     4861 ctggccattc gcgatcactg cggcgcaccg tcctgggacc tatttctggt gtcccggcag
     4921 tgcctttacc cgagaggata tcgattggaa cctccgacag cttcacgatg cgggttttgg
     4981 cttcgtccat gtcataccca tttacggtgc ccgcggggcg gagtcccaat atatccccta
     5041 tctcgcgcca cagtggatgg agatgctcga ttatcttgtt cgacgagcgg agtcgttggg
     5101 aatgtttgtg gatatgacca cagggacggg ttggtgcttt ggcgggccaa accttcccga
     5161 ttgggccatc gatgccaggg cccaatacga cgccgcatca ggggcagtta cgctggtacc
     5221 ggcaatgcag gtcaagcggg cggcgcccgg cggcgaaggt cccatgctca atcccttctc
     5281 accccgcgcc atggacttgt atctagaacg cttttcggct gcgttcgaca gctcacgtgt
     5341 ggctcgacct cgtgcgcagt atcatgactc attcgagtat cagtctaatt ggtccgcgga
     5401 tctcttcgag gagtttcgct cttgccgcgg ttatgatctg aaagagcacc tatcgctctt
     5461 cttcgatgag tctcgttccg ctgatgagga gcggacacgg ctgaagtgcg attaccgctg
     5521 gacgttggcg gagttgcacc ttgagttaat tcgccggtgg gtggcttggg cacaccaaca
     5581 tggcatgtta acgcggaatc aagcccacgg cgcgccggga aatctgctgg acttgtatgc
     5641 cgcctccgac attcccgaga cggaaatgtt cggcgcaccg gagcttccga tccccggctt
     5701 ccgtcgtgat cccgccatgg tccgcgctgc cgatgacgat ccacgcattt gccttttggc
     5761 gtcatcggcg gcccacgtgg cgcatccccg aggccagcag ttggtcagcg ccgaaacctg
     5821 cacgtggctc cgcgagcatt ggcacaccag cctggcccag atcaaattgc agttggattt
     5881 gttctttttg actggcgtta atcaactatt gtttcatggc acatgttatt cccccaagga
     5941 tgccccatgg cccggctggt tcttctacgc ctcgatcaaa atggactggc gaaacgcctt
     6001 ttggcgtgac gttccggttc taacgcaata tgtagcacga tgccagtcgg tcctgcaagc
     6061 cgggcaacat gacaatgacg tgctcctcta ctggcctatt ttcgacttgt ggatggaccc
     6121 gaaggggatg acgattccgc tgacggttca ccgcc
//
LOCUS       DAOSUT010000212         6124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6000_length_6124_cov_8.608337, whole genome shotgun sequence.
ACCESSION   DAOSUT010000212 DAOSUT010000000
VERSION     DAOSUT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_6124_cov_8.608337"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLD05_14310"
     CDS             <1..857
                     /locus_tag="PLD05_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78660.1"
                     /translation="QPVSDPSVTETPNWLLWRQSLSREEIRRPLLRSDSSSTADHASP
                     SEPKAQVLARFTTGHPFVVVHPVGQGQVVFVSGGLSREWSSLSASYAIVMFDRLLQNL
                     IERSIPRRNLETVENYRVIVNDPNTAWRLVAPDGTVTPISVGAVGPQQFGIQLNGFSQ
                     RGLHRLAPGRLLASDTQLASGPADAQMQPANSSELASREGNLSAEKEILLGIRGPREE
                     SLPDYVSESELEGILGGHGVLSSDNGFLQAANSHEWWPWFVAAALLGLLAELGILSNL
                     RSRNEVSS"
     gene            854..3364
                     /locus_tag="PLD05_14315"
     CDS             854..3364
                     /locus_tag="PLD05_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78661.1"
                     /translation="MSLSSVIGRLIGLSQLESIETIGLRWNANWMVGGGGWLFFALLI
                     VVGLVVFFYTRQSLGQRRLARVLLPVARSLALVGLVVILAQPVIVMKVRHAVRPYLWF
                     VIDDTASMAIRDEIASQEDKTEQFREPANPNSTGVPGVFGNAQEVSRTEKVKEVLFGR
                     GEKTLAELAKFYRLRFFAMRRIDGLDLLPQSDSLDAASLNSLRDELKASGDMTALGAA
                     LADVLRRKAGVQLAGIALVSDFNHNSGPPPEVFASRLDVPIFSIGVGRPESQDVAVDL
                     QLPPFMKKNERSALTVVLRQQGFAGEQVQLRVSVQRPGQPPSEQGPGVPKSPSTSGGE
                     ELSTSSGNDGSLILERTVNLSDRVVTIEVPFTPAETGQHLFVAEADVLPTEKLPQNNR
                     VTRDTFVREYFVRVLFIEDEPTWEWRFMREVFHRDPLVGPEGFRTYLRSADPQVRERN
                     PLFVKSLAIPRRDFFTNDVIILGDIPVSAMSPATAEMIQEFVNDMGGGLVFCVGARYG
                     PGQWTETPLGQLLPVEVPPLTRLRETTFAPQLTAEASLFDFMRLGESQQESAKAWNNL
                     GKLAWYYPVQRVRPLATVLACHPYDTCLDGKTRQPLIALQKVGRGEVVYIGTNEMWRL
                     RRKFGEKYYRQFWGQMIHRLAIQHALGAEKRFVVRSDHREYRTGDEAVITVEAYDADF
                     HPLDAAQLSESWLVGTLFVPTESQHQVREIKLTQTRPGWFETRIPLYNAGEYRLVVKD
                     PLANQEVETRFVVSHQSVEFQQPTRNVERQRLLAQLTGGKSYELSEWTNLLTDLPQEP
                     RTETVIRELEIWNTWPFFIFVVGLLLCEWFLRKWLNLR"
     gene            3346..5139
                     /locus_tag="PLD05_14320"
     CDS             3346..5139
                     /locus_tag="PLD05_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78662.1"
                     /translation="MVESAVRSELAPLHRLLGRIGRKRQRLRLTIGILGVLVGLIFVA
                     LGLFTLDWLLGLTIAQRVVLILVAIIVAYVASRRFILPWFIGRESELDLALLVQKHFG
                     IDSDLVAALQFEYPEAVEWGSVELEQKVIRQVAAQAQQLPLIEDVPRAPLRKRLLASL
                     CGVTLVTALVLLFPEHFAVFVKRLMLQNVHYPTATRIIDLSVNGQPVSWDKAGQTLRC
                     GVGATVDIRVVATGRLPEQGQALFQIVDQRRNLRIPLVPVENQPADSPAVQRAFSGKW
                     TGLDAPCRVRMTLGDATSQWIELSVVPPPVVLTWFAFQDPLSENPEYTMAVGNLQISV
                     REGTQVIVGLASRSELKHAVATIGLLEVPLQRGIPQHLEGLLSAISQGAAASIPQTSG
                     PINSRLPSASEFWWLDPAGTPLERVESPIRIRFQVRDQFGLEPSVPVEAFVGVRPDYP
                     PQIAVRTVTRLVLPSAHPTLFVEARDDVALREVRALGKVVQADGEEGGQYEWVLWQGN
                     GPPTRTLNEKYKVDLSQLKAKKGDKIELTVVAEDERGSDRPGAVTQADAIFLEVTDLA
                     GILAVMAEADRESAAQLQEMIDRQLDVGGEQ"
     gene            5136..>6124
                     /locus_tag="PLD05_14325"
     CDS             5136..>6124
                     /locus_tag="PLD05_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78663.1"
                     /translation="MKALGLHKRTQDIMGFLAPRKTGEPVPTVVPFLLALLMMLPQLT
                     GAIWAEIGTQSPGSSGQSTGVEVDQGGSTPRQATEVVGWQLTPTDTLRKQNLQQQVRQ
                     MAKRLVAERLNEQILQLEENGLTNLPLYSELREMQSHLDALVETHMKEVLLSLEQLIQ
                     APPEKQPALFQIAREKSRNVVVQLLIERQKILRRLRIAEIEERVIRLIERETSILEKT
                     ERSLAESAVRQAASAVALKEDQQDIYTLFRGMKQSLEEIRTWGGRVGTEATAALQSLT
                     ESRVDGLFEQSIQQLSENQFAQAAHVERQIITSLEALLERVRRFQGEMESDSR"
BASE COUNT         1330 a         1653 c         1739 g         1402 t
ORIGIN      
        1 cccagccagt cagcgatcca tcggtgacgg aaacgcccaa ttggttgctc tggaggcaat
       61 ctctaagtag ggaagaaatc cggagaccac ttttgcgtag cgattcctca tccaccgcag
      121 atcacgcaag cccatcggag ccaaaagccc aggtcttggc ccgctttacc actggtcatc
      181 ccttcgtcgt ggtacacccc gtgggccagg gacaggttgt tttcgtcagc ggtggcctct
      241 cacgcgagtg gagcagccta tcggccagtt acgcaatcgt catgttcgac cgattgctcc
      301 agaaccttat cgagcgctcc attccgcgac ggaacctcga aactgtggaa aactatcgtg
      361 tcatcgtgaa tgacccgaat accgcgtggc gtcttgtggc gcccgatggt accgtgaccc
      421 ccatttctgt gggcgccgtg ggaccacaac aattcggcat acaactgaac ggattttctc
      481 agcgtgggtt gcatcgcttg gcaccgggta ggttattggc ttccgacacc caacttgctt
      541 ccggcccggc tgatgctcag atgcaaccgg caaattcctc cgagcttgct tcccgggagg
      601 ggaatctatc cgccgaaaag gaaattctcc tgggcattcg cggacctcgt gaagaatcgt
      661 tgcccgatta tgtttcggaa tcggagctcg aaggcattct cggcggtcat ggagtgcttt
      721 cttcggacaa cggctttttg caagcggcca atagccacga atggtggcca tggttcgtcg
      781 ccgcggcact gttgggactc ttagctgaat tgggaatcct aagcaacctt cgttcgcgca
      841 acgaggtttc atcgtgagtc tatcctcggt tattggacgg ctgatagggc tttctcagtt
      901 ggagtcgatc gagacgatcg ggctccgctg gaatgccaac tggatggtag gcggtggcgg
      961 ttggctcttc tttgcgctac ttatcgtagt gggattggtc gtgtttttct acacacggca
     1021 atcgttaggc cagcgacgtt tggcccgtgt tcttttgccc gtggcccgat cattggcctt
     1081 ggtcggcctg gtcgtgatcc tcgcccagcc tgtgatcgta atgaaggttc gccacgccgt
     1141 tcgtccttat ttgtggtttg tcattgacga cacggcgagt atggccatcc gcgatgaaat
     1201 tgcatcacag gaggataaaa cagaacaatt ccgagagccc gctaacccaa actcgaccgg
     1261 tgtccccggc gtcttcggca atgcccaaga ggtttcacgg actgagaagg ttaaagaagt
     1321 cctttttggc cgtggcgaaa aaaccttggc tgagctggcc aaattttatc gccttcggtt
     1381 ttttgcgatg cgtcggattg acgggctcga tctgctgcca cagtcggact ctcttgatgc
     1441 ggcttcatta aatagccttc gcgacgaact gaaggccagt ggtgacatga cggccttggg
     1501 agccgctctg gccgatgtcc ttaggcggaa ggccggagtt cagttggctg gtattgcgct
     1561 ggtcagtgat ttcaaccaca actctggacc accgccggag gtttttgcct ctcgcctgga
     1621 tgtgcccata ttttccatcg gggtcggacg accggagtcc caggatgttg cggtggattt
     1681 gcaattgccg ccgtttatga aaaaaaacga aaggtctgcc ttgacggttg tgcttcgtca
     1741 gcaggggttc gccggagagc aggtccaatt aagagtgtca gtccagagac cgggacagcc
     1801 cccaagtgag caaggccccg gggtgccgaa aagtccttcg acatccggcg gcgaggaact
     1861 gtcaacctct tccggaaacg acggatcatt gatcttggag cgaacagtta acctgagcga
     1921 ccgggttgtc acgatagagg tcccatttac tcctgccgaa acgggacagc atcttttcgt
     1981 ggcagaggcc gacgtcttac ccacggaaaa attacctcaa aacaaccgcg tgactcggga
     2041 tacttttgtg cgagagtact tcgtccgcgt ccttttcatc gaggacgaac cgacctggga
     2101 atggaggttc atgcgcgagg tctttcaccg cgatcccctt gtcggaccag agggttttcg
     2161 gacctacttg cggtccgctg atccgcaggt ccgcgaaagg aacccactct ttgtaaaaag
     2221 ccttgccatt ccccgccgcg actttttcac gaatgacgtg attatcctgg gagatattcc
     2281 tgtctcggca atgagtcctg ccacggcgga aatgatccaa gagttcgtaa acgacatggg
     2341 cggtggtctg gtgttctgtg tcggagcacg ctacggccct ggtcaatgga ctgaaacgcc
     2401 actgggacaa ttgctgccgg ttgaagttcc accgctgaca cgtctgcggg aaacgacatt
     2461 tgccccgcag ttgacggccg aggcaagtct tttcgatttc atgcgcttgg gagaatccca
     2521 gcaagaatcc gcgaaggcat ggaacaatct cggaaagctc gcttggtatt atcccgtaca
     2581 gcgtgttcgc cccctggcca ccgtattggc ctgtcatcct tacgatacgt gtcttgacgg
     2641 gaagactcgg caaccgctca tcgcccttca gaaggttgga cggggtgaag ttgtctatat
     2701 cggtaccaac gaaatgtgga gactgcgtcg aaagttcggg gaaaagtact accgacagtt
     2761 ttgggggcaa atgatccatc ggctggctat tcaacatgcc ctaggggccg agaagcgatt
     2821 cgttgttcgt tctgatcatc gtgaataccg cacgggcgat gaggccgtca ttaccgttga
     2881 ggcttacgac gccgattttc accccttgga tgccgcgcaa ctttccgaat cgtggttggt
     2941 tggaacgctc tttgttccca cagagtcgca gcaccaggtg cgcgagatta aattaactca
     3001 aacgcggccg gggtggtttg aaactcgtat cccgttgtac aacgccggtg aataccgtct
     3061 agttgtgaag gaccctttgg caaaccaaga ggtggaaaca cgttttgtcg tgtcgcatca
     3121 gtccgtcgag tttcaacagc cgacccgaaa tgtcgaacga cagcgcctgc tggctcaact
     3181 gaccgggggg aaaagttacg aactgtccga gtggacgaat cttttgacag atttgcccca
     3241 agagccgcga actgaaacgg tgattcgtga attagaaatc tggaatacct ggccattctt
     3301 catattcgtc gtgggattgt tactttgcga gtggtttctg cgcaaatggt tgaatctgcg
     3361 gtaagaagcg aactggcgcc gctccaccga cttttgggcc gaattggccg caagcgacag
     3421 cggcttcggc tgaccatcgg catcctcggg gtgttggttg gtctcatttt tgtcgctttg
     3481 gggctcttta cattggattg gctcttgggc ctgacaattg cccagcgagt cgttctgatt
     3541 cttgttgcta tcatagtcgc ctatgtggca tcgcggcggt ttattttgcc atggtttatc
     3601 ggtcgcgagt ccgaattgga cctcgccttg ctggttcaaa aacacttcgg catcgacagt
     3661 gacctggtgg cagcgctgca gtttgagtat ccagaagccg tcgaatgggg ctccgtcgag
     3721 cttgagcaaa aggtcatccg gcaagtggca gcccaggcac agcaactccc tttgattgag
     3781 gacgtgccgc gggcaccttt gcgaaaacgg ctgctggcaa gtctttgcgg tgtaaccctg
     3841 gtaaccgccc ttgttttgct attccccgaa cattttgcgg tctttgttaa acggctgatg
     3901 ttgcaaaacg ttcattatcc gacggcaaca aggatcattg acctgtccgt caatgggcag
     3961 cccgttagct gggataaagc tggtcaaacc ctccggtgcg gtgtgggcgc aacggtcgat
     4021 attcgcgtcg ttgccacggg aagactcccg gaacaaggtc aggccctttt tcaaatcgtt
     4081 gatcaacgcc gtaacctgag gattcctctc gtaccggtag agaatcaacc tgccgattct
     4141 ccggctgtgc aaagggcgtt ctcaggaaaa tggacggggc tggatgctcc ttgccgggtt
     4201 cgtatgacgt tgggcgacgc cacatcgcaa tggatcgagt tatccgttgt tcccccgccg
     4261 gtcgtcctga cctggtttgc ctttcaggac ccactgagcg agaatccgga atacacaatg
     4321 gcagtgggaa acctccagat ttccgtccgg gaaggcactc aggtgattgt gggcttggcc
     4381 tcccggtcgg aattaaaaca cgctgtagca actatcggcc tgttggaagt tccccttcaa
     4441 cgaggaattc cccagcacct ggaaggcctg ctatccgcca tttcgcaggg agcagcagcc
     4501 agtatccccc aaacaagtgg ccccattaat agccggctac cttcggcctc tgagttttgg
     4561 tggctcgacc ctgcaggtac gccgcttgaa cgcgtggaat cccccattcg cattcgcttc
     4621 caggtccggg atcagtttgg gctagagccg tcggttcccg tcgaggcgtt tgtcggtgtc
     4681 cgccccgatt atccacccca gattgcagtc cgtaccgtca ctcggcttgt tttgccctcg
     4741 gcacatccga ctctctttgt ggaagcacga gacgatgtcg ccctgcgaga agtccgcgcg
     4801 cttggcaagg tcgttcaagc ggatggcgag gaaggaggac agtatgagtg ggtcctgtgg
     4861 caggggaacg gcccgcccac gcgaacgctc aacgagaaat ataaagtcga tttaagtcag
     4921 ttaaaagcca aaaaaggtga taaaatcgaa ctgaccgtgg tggctgaaga tgaacgtgga
     4981 agcgaccggc cgggcgccgt gacccaagcc gacgccatct tcctagaagt aacagacctg
     5041 gccggtattt tggccgtgat ggccgaggcc gatcgagaat ccgcggctca gctccaagag
     5101 atgatcgatc gccagcttga cgttggaggg gaacaatgaa agccttgggc ttacacaaac
     5161 ggacccagga tatcatgggc ttcctagcac cacgcaagac gggggagcca gttccgacgg
     5221 ttgtcccgtt tctacttgcc ctccttatga tgttgcccca actcaccgga gccatctggg
     5281 cagaaatcgg gacgcaaagc cctgggtcgt ccggacaatc gacaggggtg gaagtggatc
     5341 aaggcgggag caccccccga caggcaacag aagtggtcgg gtggcagttg acacccacag
     5401 acactctccg aaagcagaac ctccagcaac aggtccgcca gatggccaaa cggcttgttg
     5461 ccgaacgtct caacgagcaa attctgcaac tcgaggaaaa tgggctgaca aacttgccgc
     5521 tatattccga gctgcgggaa atgcagtcgc atctcgacgc gctggttgag acgcatatga
     5581 aggaagtcct actgtctctg gaacaactta ttcaagcacc gcccgaaaaa cagcccgcgc
     5641 ttttccagat cgccagagag aagagtcgca atgtagtggt tcagttactg attgagcggc
     5701 agaaaatctt gcgccgcctg cggatcgccg aaattgagga gcgagtcata cggttgatcg
     5761 aacgggagac aagtattttg gaaaaaaccg aaaggtcact ggccgaaagt gccgtgcgac
     5821 aagccgcctc agccgtggcc cttaaggaag atcaacaaga catttacaca ctttttcgcg
     5881 gcatgaaaca atctctggag gaaatccgaa catggggcgg acgggtcggt acggaagcca
     5941 cagccgctct ccagtcactt acagaatctc gcgttgacgg tctttttgag caatcaattc
     6001 agcagctttc ggagaaccaa ttcgcgcaag ccgctcacgt cgagcgccag attattacct
     6061 cgctagaagc tctcctggag cgagttcgcc gcttccaggg cgaaatggaa agtgattccc
     6121 gcga
//
LOCUS       DAOSUT010000213         6037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6090_length_6037_cov_11.087095, whole genome shotgun sequence.
ACCESSION   DAOSUT010000213 DAOSUT010000000
VERSION     DAOSUT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_6037_cov_11.087095"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..795
                     /gene="rimI"
                     /locus_tag="PLD05_14330"
     CDS             280..795
                     /gene="rimI"
                     /locus_tag="PLD05_14330"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOP78664.1"
                     /translation="MSVKSQSGVRTLVRWMLREDLQAVLKIEHESFEFPWTESEFLAC
                     IQRPNCIAMVAVVQEKVVGYMIYEMSPQSFQILNVAVDAEYRRNGVGRALVQRLINKL
                     SPQRRHKISLEVRETNLPAQLFFKALGFQAKAILHDHYPDTAEDAYLMTYEIAPQVNP
                     GFRNRLAKFFR"
     gene            complement(864..1445)
                     /locus_tag="PLD05_14335"
     CDS             complement(864..1445)
                     /locus_tag="PLD05_14335"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP78665.1"
                     /translation="MTNPADKVSFTELVAAAGRGDEEALTRLLEYCEPKFRIIARVSL
                     GPLLRPQLDTMDLVQSMKRMLIPGLRAGLYELDSPDQLVALAATIIRRKVALYWRRQK
                     KQAVLRLTEQALTGEEDALVEHRIQDWENEELLARLLESLTEEERQLFRMQLEGLTIV
                     EIARHLGCRPGPLRARLSRLRKKLRSLAGISWS"
     gene            1666..2460
                     /locus_tag="PLD05_14340"
     CDS             1666..2460
                     /locus_tag="PLD05_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOP78666.1"
                     /translation="MIPIVSIKNLSLRLSGQEILRDISFDLQAGDYVCLVGPNGSGKT
                     SVLRCVTRVYSRWLGEITVLGKPVRSFSQRELARHLAYLPQADGIAPPYTVEDFVLMA
                     RYPFVNPFRPPSAEDREAVEMALRMTELWSLRHRVVRTLSGGERQKVYLAGAIAQQAK
                     VLLLDEPTTFLDVPHQEDVRSLLHRINRQSGVTILAATHDLNAAVLSANRILALREGR
                     LLFSGSPEEFMTTEVVRQVFGFAPQFVRHPQVPLPMIVPSPGGGAT"
     gene            2457..3458
                     /locus_tag="PLD05_14345"
     CDS             2457..3458
                     /locus_tag="PLD05_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOP78667.1"
                     /translation="MNRWAGGFLVIVAVALVVLGVTPFAGGNWIVPWRATGPESLVAR
                     QILFELRIPRVLIGFVAGAALAVGGVAFQAMFRNPLATPYTLGVASGASLGAVISFQC
                     GLAFSTWGFSTTAVFAFLGAMATLLVVYAVAMWRAELSVATMLLAGVAMSFFNSSLIL
                     ILQYLGDPVQVFHAFRWMLGGLEGTIGYDDLKVLLPVVLLSSVVVFALRRELNLLALG
                     DEWATARGVPLMKIKALLLFVTGSMVASVVALCGPIGFVCLIAPHITRLLVGPEHGRL
                     IPAAALLGGAFLVVCDTVARTVLAPTEIPVGVITALLGGPFFLWLLFTRPLSWEWLS"
     gene            3675..4679
                     /locus_tag="PLD05_14350"
     CDS             3675..4679
                     /locus_tag="PLD05_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP78668.1"
                     /translation="MEMRFLGDTGVRVSVLCLGTMTFGKNQWDFGDLKLPAVKEMVHA
                     CLDAGVNFIDTADVYSLGESEELLGQAIEGIRKDLIIATKVRMRMSNKPNEIGLSRHH
                     ILNSCDASLKRLRTDYIDLYQVHIWDPVTPLEETLRALDDLVRWGKVRYIGVSNFAAW
                     QVMKALAISDKHDWVRFQSLQALYNLAFRDIEVELVPLCQDQHLGILPWSPLAFGLLT
                     GKYRRGEPIPEGTRMQGPLKDFLPTDWDRLYKIVDVLREIASGHGVPIAAVALAWLIH
                     KPAVTSVIIGARTMDQLRENLKAAEVKLGPDEMKRLDEVSAIPAPYPQWMIATVGQDR
                     "
     gene            4868..>6037
                     /locus_tag="PLD05_14355"
     CDS             4868..>6037
                     /locus_tag="PLD05_14355"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOP78669.1"
                     /translation="MSGWVMCFAEWIKFVCLVSMITAVSLVAGPWDAIANDGPRMTQL
                     PRLVWLASEVPRWKKIEGRIDWPKTYVNPFDPAEVTVDVVVTAPDGKTFLQPAFWMED
                     FVWRSMGRSRGGEWFYPQGRPFWMVRLTPTLPGTYHVRARVKDRDGTAESSVATFRCQ
                     DAPGHGFLRVAKSDPRFFAFDDGTPFFAIGQNLAFIGSNSQYFTLRGAIEAFTRLADN
                     GANFLRIWTCCDDWAMCIEGRKSAWGRSWSWRPSWVDRPEYVPQPDFRQVPPFAHLAV
                     NKVIRLTPEAVRPDPSHRVAVQPNTSYVWSLAVWADRMTEIRSRILGKEVVFSLGADE
                     IKRWVVKEATLTSADDAWWLGPVEISLGQTGGTRPEVYLAGMSLRDAATDRELLWE"
BASE COUNT         1150 a         1584 c         1849 g         1454 t
ORIGIN      
        1 gcgtccccat gttgtggttt gggccgtctt gaagtgcgtc aatggctctc tcctggaacc
       61 acttgcgcgg ggcctttttg catagtccat ggcgagacaa aacgagccgc agacgctgga
      121 aaaacagtct tggcgggatt gcggagttgt gctaatctat tgcctatggt tggggcaagg
      181 cgacgaccaa acttcagctc aaacggcagg aggggtaagc cccataatgc gtcggctgct
      241 gggaggtatc ttgactctgg ctaacaagga aggtttgcca tgagtgttaa atctcagagc
      301 ggggtgcgaa ccctcgtccg ctggatgctc agggaggacc tccaggctgt cctcaagatt
      361 gagcacgaaa gcttcgagtt tccttggact gagtctgaat tcttggcgtg cattcagcga
      421 ccgaattgca ttgccatggt cgcggtggtt caggaaaagg ttgtgggcta catgatttac
      481 gagatgagcc cacaatcgtt ccagattctc aatgtggccg tcgatgccga ataccgccgg
      541 aacggtgttg gccgggcctt ggtgcagcga ttgatcaaca agctttcgcc gcagcggcga
      601 cataagatca gtctagaggt tcgggaaact aatctcccgg cgcagttgtt tttcaaggcc
      661 ctgggatttc aggccaaggc catcctccac gatcattacc ctgacaccgc cgaggacgcc
      721 tatttgatga cctatgaaat tgccccccag gtcaaccccg gcttccggaa tcgtttggcc
      781 aagttttttc gataactgag aaacttcgga agcgcgctaa acggagagta gcgatttcac
      841 ggccgatgag ggatcgccag ggatcaactc caagaaatgc cggccaggct ccgcagcttc
      901 ttccgtaaac ggctcaatcg ggcgcggagt ggtccagggc gacaacccaa gtggcgggca
      961 atttctacga ttgtcagccc ttctagttgc attcggaaaa gttggcgttc ttcctccgtt
     1021 aaggattcca gcaatcgtgc gaggagctct tcattttccc agtcttggat gcggtgctca
     1081 acgagggcat cttcttcgcc cgtgagtgct tgttctgtaa ggcggagcac agcctgtttc
     1141 ttttgacgcc gccagtacag ggccaccttt cgccggatga tcgtggccgc caaggccacc
     1201 agttggtcag gggaatctaa ttcgtaaagt ccggctcgca agccgggaat gagcatccgc
     1261 ttcatcgatt gaacaaggtc catggtatcg agttgcggac gaagcaaagg accgagcgag
     1321 acccgggcta tgatccggaa cttgggctca cagtactcca acaggcgggt tagggcctct
     1381 tcatcacctc gtccggcagc agcgacgagc tctgtgaagg ataccttgtc agcaggattg
     1441 gtcacaaccg aacccttccc atcgcgtggc gccgattgcc ctattaaacg cgcatcgaaa
     1501 gacgtacttc gagagcgcgt cgccagggca accacctgac acaacccaac gcttcttttg
     1561 ctttaagtat aatcgaaggt gacacccatt tcacccggct gcccgtgttc cctcggcaaa
     1621 agccagggtc aattttaccc gagcaacagg aatgccgtta ccatcgtgat accgatcgtc
     1681 tccataaaaa atctgagctt acgcctatca gggcaggaaa ttctccgcga tatttctttt
     1741 gacttacaag cgggggatta cgtttgcctc gtgggcccca atggttcggg gaaaacgagt
     1801 gtgctgcggt gtgtgacgcg ggtctattcg cgatggctgg gggaaataac ggttttgggt
     1861 aaaccagttc ggagtttttc acaaagggag ttggcgcgtc atttggctta tttacctcaa
     1921 gctgatggaa ttgccccgcc ctatacggtg gaagattttg ttctgatggc ccggtatcca
     1981 ttcgtcaacc cctttcggcc accgagtgca gaagatcgag aggcggtcga gatggccctg
     2041 cggatgaccg aactgtggtc gttgcggcat cgtgtggtga gaacgctgag cggtggggaa
     2101 cgccagaagg tgtatctggc cggggccatt gcccaacagg caaaggtcct cctcttggac
     2161 gaaccaacaa cgtttctcga tgtgccgcat caggaggatg tgcgttccct gctccaccgt
     2221 attaatcgcc agtcgggcgt cacaatcttg gcggcaacac acgacttgaa tgcggccgtc
     2281 ctttcggcca ataggatttt ggcgttgcgg gaagggaggc ttctcttttc cggttcgccc
     2341 gaagaattca tgaccaccga ggtggtgcga caggtctttg gatttgcgcc gcaatttgtc
     2401 cgccatcccc aggtgccact gccgatgatt gtcccgtcac ccgggggagg tgccacgtga
     2461 accgatgggc gggcggtttc ctagtcatcg tggctgttgc cctagtggtg ttgggcgtga
     2521 caccttttgc cggcggcaat tggattgtcc cctggagggc aacgggaccg gaaagcctgg
     2581 tggcgcgaca aatcctcttt gagttgcgga ttcctcgggt attgatcgga tttgtggccg
     2641 gtgcggctct ggccgtgggc ggtgtggctt ttcaggcgat gtttcggaat cccttagcca
     2701 caccatacac cttgggagtg gccagcgggg cgtcgctggg ggctgtgatc agttttcagt
     2761 gcggattggc cttttcaaca tggggttttt caaccactgc cgtttttgcc ttcttggggg
     2821 ctatggcgac gcttctggtt gtttatgccg tggccatgtg gcgggctgag ctgtcggtgg
     2881 cgaccatgct gttggccggt gtggcgatga gctttttcaa cagcagtttg attctcattc
     2941 tgcaatacct gggcgatccg gtgcaggtgt tccatgcctt tcgctggatg ctcggaggtt
     3001 tggaaggcac catcggctac gacgatctta aggtgctgtt acctgtcgtc ctgctcagct
     3061 cggttgttgt gttcgctttg cggcgggagc tgaatctgct cgcactgggc gacgagtggg
     3121 caacggctcg cggtgttccg cttatgaaga tcaaggccct gcttttgttt gtgacgggca
     3181 gcatggttgc gtccgtggtg gcgttgtgcg gtccgattgg gttcgtgtgt ttgatcgctc
     3241 cacacatcac acgtcttttg gtgggaccag agcatggtcg gctcattccg gcggcggccc
     3301 tgctgggggg tgcatttttg gtagtctgcg acacagtggc gcgaaccgtc cttgctccta
     3361 cggagattcc cgtcggtgtc atcacggccc tgctcggtgg acctttcttc ctttggttgc
     3421 ttttcacgcg gccactttcc tgggaatggc tttcgtaacg cctcggcatt cgcgactacg
     3481 agcccatgcg gccagggcag tgggtggttc cccgtcattg gtgtagcctg ttgcgactct
     3541 ggaacttggc cgacaaaccg ttacaattcg taccaaggcc ggtcacgctg ggaggacgtg
     3601 acaggttggt cgggatcgga tccgactgtg ggaagcagcg agtattgggt tctcggtcaa
     3661 gaggagatga actcatggaa atgcgatttc tcggtgatac gggggtgcga gtttcggtgc
     3721 tttgcttggg taccatgacg tttggaaaga accagtggga tttcggcgac ctgaagctgc
     3781 ccgcggttaa ggagatggtc cacgcctgcc tggacgcggg ggtcaatttt atcgacacag
     3841 cggacgtgta ttcgctgggc gaatcggagg agctcctggg gcaggcgatt gagggaattc
     3901 gcaaggattt gatcattgcg acaaaggtcc gcatgaggat gagcaacaag cccaacgaaa
     3961 tcggcctctc acgccaccac atcctcaatt cctgtgatgc gagtctcaag cggttgcgga
     4021 ccgattacat cgacctttat caggtccaca tttgggaccc agtgactcca ctggaagaga
     4081 cgttgcgggc cttggacgat ttggtccgtt gggggaaggt gcgttacatt ggcgtttcga
     4141 actttgccgc gtggcaggtg atgaaggccc tggcgatttc tgataagcac gactgggttc
     4201 gctttcaatc gttacaggcc ttgtacaatc tcgcctttcg tgacattgag gtggagctcg
     4261 tcccactttg ccaggaccaa cacctgggga ttttgccctg gagtccgctg gcctttggcc
     4321 tcttgaccgg gaaataccgt cgcggcgagc ccatcccgga agggacacgc atgcaaggtc
     4381 ctctaaagga ctttttgccc acagactggg atcggctcta caagattgtc gatgtcctac
     4441 gggagattgc ctcggggcac ggcgtgccta ttgcggcggt tgcactggct tggctgattc
     4501 acaagcccgc cgtaacctcc gtcatcattg gtgcccgcac catggatcaa ttgcgggaga
     4561 acctcaaggc tgcggaggtc aagttgggac ccgatgagat gaaacgcctc gacgaagtga
     4621 gtgccatccc cgcaccatat ccgcagtgga tgattgctac ggtcgggcaa gatcggtaga
     4681 ctaggccgcg tgaagagcaa gggttccagc ccggttgctg gacgcatccc gggggcggct
     4741 gcgcctagcg attgggtttt ggttgacgga ctacggacgt gggagctgct tcgccgcaga
     4801 ttctcgcaga tttttgtcac gtttaatttt tctgtttcgc cggtttaatg cccgcggagt
     4861 gacttccgtg agtggttggg ttatgtgctt tgccgaatgg atcaagtttg tctgtctcgt
     4921 ctcaatgata acggctgtga gcctcgttgc tgggccgtgg gatgccatcg ccaacgatgg
     4981 gccacgaatg acccaattgc cacgcttggt gtggttagcc tcggaggtcc cgcgttggaa
     5041 gaagatcgag ggtcgcatcg actggcccaa gacctatgtc aatccatttg atcccgccga
     5101 ggtgactgtt gatgttgtgg tgacggctcc ggatggtaag accttccttc agccggcctt
     5161 ttggatggaa gattttgtct ggcgatcgat ggggcgttcg cgcggaggcg aatggttcta
     5221 tccccaaggc aggccctttt ggatggtaag attgacgccc acgcttccag gcacctatca
     5281 tgtgagggcc cgcgtcaaag accgggacgg gacggcagaa tcgagcgtgg caaccttccg
     5341 atgtcaggac gcccccggtc acggttttct ccgcgtggca aagagcgatc cacgattctt
     5401 cgccttcgac gacggtacac cgttttttgc aattgggcag aatcttgcct tcatcgggag
     5461 caatagccag tatttcacgt taaggggtgc catcgaggcc ttcacacgcc tggccgacaa
     5521 cggagcgaac ttccttcgca tttggacgtg ttgtgacgac tgggccatgt gcattgaagg
     5581 tcgcaagagt gcctggggcc gcagttggtc ctggcgtcca tcgtgggtcg atcggcctga
     5641 gtacgtcccc caacccgatt ttcgccaagt gcctccgttt gcccatctcg ccgtcaacaa
     5701 agtgattcga ctaactcccg aggcagtgcg gcccgatcct tcccatcggg tggcggttca
     5761 gcccaacacc tcgtacgtct ggtcactcgc cgtgtgggca gatcgaatga ctgagattcg
     5821 ctcgcggatt ctaggaaaag aagtcgtgtt ttcgctcggg gccgacgaga tcaaacgttg
     5881 ggtcgtcaaa gaggcaacgc tcacttcggc ggatgacgct tggtggcttg gccctgtgga
     5941 aatctcactg gggcaaacgg gcgggactcg tcccgaggtg tatcttgccg ggatgtccct
     6001 gcgtgatgcg gcaacagatc gggagctcct ttgggaa
//
LOCUS       DAOSUT010000214         6032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6096_length_6032_cov_10.060231, whole genome shotgun sequence.
ACCESSION   DAOSUT010000214 DAOSUT010000000
VERSION     DAOSUT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_6032_cov_10.060231"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1316)
                     /locus_tag="PLD05_14360"
     CDS             complement(210..1316)
                     /locus_tag="PLD05_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOP78670.1"
                     /translation="MRAHSRFKRTCVQLLKIAASVAILAWLVWDSVRRGEGLFSDEHG
                     FQPEKLWALVRHAVDNWLILLLAFICAFASVMITFIRWWLLIRSVGVPISLRESLRFS
                     FVGYLLNLAPMGIVGGDLAKAVLVARHCPEHRAPSVASVFVDRVVGLYVLLLVASGAI
                     LLTNFHATGPAIVQSACRLTLVVSVLGTIVLLLVWAPDWTGGRVRRLWEKLPVVGRPI
                     ARFTEAFRAYGREPGTLLLSLVMTLPVHLLFAIVVFLVGTALFPKVHSLDRHFVFAPL
                     AGVMQVLPISVGPAEFVLDRLFVLTPTADGSIIPPGQGLVTLLVYRVFNLLISTVGVV
                     YFLMAREEWREALHEVEEAEEPPRALEIEAPKPN"
     gene            complement(1386..2378)
                     /gene="gmd"
                     /locus_tag="PLD05_14365"
     CDS             complement(1386..2378)
                     /gene="gmd"
                     /locus_tag="PLD05_14365"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOP78671.1"
                     /translation="MTKRALITGITGQDGSYLAEFLLSKRYEVHGMVRRASTEGFERI
                     QHIRDQLHLHQADLLDQLSLVRLVAEVRPHEVYNLAAMSFVPTSWQQPLLTGEFTALG
                     VTRMLEAIRHVDPTIRFYQASSSEMFGAVREEPQNENTPFYPRSPYGVAKVYGHWITV
                     NYRESFGVFACCGILFNHESPRRGKEFVTRKVTDAVARIKLGVQDKLYLGNLDAMRDW
                     GFAGDYVEAMWLMLQQPKPDDYVIATGEKHSVRELVELAFDYAGLDWKRYVEIDPKLI
                     RPAEVYTLRGDASKARRVLQWSPKVSFPELVKMMVDADLERVRREIADRAHRLP"
     gene            complement(2982..6008)
                     /locus_tag="PLD05_14370"
     CDS             complement(2982..6008)
                     /locus_tag="PLD05_14370"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOP78672.1"
                     /translation="MSFRRPSRATRRLVIESLEQRHLLSVTSLPLPGQFLDLGPVDYS
                     QVTLADSSSAGQWFRLTAQNTATLTAICGPSPADPSPLGVTVYAVSGNALVELARGNG
                     RVDTPVTAGSTYFVWLGQVQSGEQLFLANLVQNSGTTVTVIGTAGDNTFEFDGTSRLT
                     VNRITYQFSTAWQRLSINAGLGEDSATIVTGLNSENIVYTPGRVVVSTPSRSIEVSGI
                     ENLTLYASPSAVAEVNDSPADDDSVIFPQRVSISGGGFSATIVGPQTIHVYARNGGQD
                     AITFYDSPQADQASYKNGVAILQGAGFYNRAKFFESVTFVGTPGAEDMASLVGTKGSD
                     RLSGRLGDITLTAGGTNVRAVNFWTTIVRSAGGTDTAQLDDSPQDDWVFSFAGDVSLF
                     NMDMTVRVYGFAFTHIYSRYGGFDTAYFYDSPRDDLFVGRQDFSSFRTDPYFSRVKFF
                     DAVYAYSRSGGYDTAKLYDSSGNDELTIRPLYVRLEGPGFSNRVETFANVVAYSTGGG
                     TDRVFGYGSDGKDQLTVASSATQFFLAETQILVTARGFKQEAYSLDATDEVNGAAPRV
                     KINREESSTVIYAIDYFDPSSPTLGFQRAIDALPDEGGVVILPAGVFELRQGLVLRSN
                     VTLRGSDSGTTLRRAPLLNAFVTTEARPGDRRITVESTAGFRVGDEIALVSQLTTFDT
                     REKTFVIVGIEGNDLILDRPIPEAGYSPTNKAEVVNVFSLVSVNGTVTAPAVNAKIEN
                     VRLDGNLRENYRRWRVVLPATLSVVYAANTTIKNVTVVRSPATGIMLEQGHDNLLENC
                     TVENSRRDGISLVWETDSIVRRCTVRGSGYGMADISDWGEGILVSGGRDIRVENCLTE
                     YNLGKGLHPAGDLTIGGLWINNVSRYNGGNGFHYCFNNFGIWAVGNELYGNGYYGIGG
                     LGLGGDYGDRFNVVMNNVIYNNQRNGIVVNGGRDNFILNNEIRSNSQLTPGRYAEIVL
                     GPVYTTVVEGNKITPTLNAQAIDAFYVRLFNSIRDL"
BASE COUNT         1411 a         1719 c         1632 g         1270 t
ORIGIN      
        1 acgtccggcg aagagcacac aagtggccca ttgtctctta agaacctgtc ccatactctc
       61 ccctcgcccg ttccgcggga gaggggccgg gggtgagggt ggctcgaatc gctgccggtc
      121 ttcttaatcg attcccccgt tcgcttgccc tcattccaac cctctcccgg agggagaggg
      181 ggactttttg ggataggctc taaagtacgt caatttggtt taggggcctc gatttccaag
      241 gccctcggcg gttcttctgc ttcttccacc tcgtgaaggg cctcgcgcca ttcctcccgc
      301 gccatcagaa aataaacaac ccccacagtg gagatcaaca aattgaagac acgataaacc
      361 aggagcgtga ccagcccctg accggggggg attatgctgc cgtcggccgt cggtgttagg
      421 acgaacaagc gatcgagaac gaattcggct ggtcccacgg agatcggcag gacctgcatg
      481 acccctgcca ggggggcgaa gacgaaatgc cggtcgaggc tgtggacttt cggaaacagg
      541 gccgttccca cgagaaacac cacaatggca aataggaggt gcacggggag cgtcatgacc
      601 agcgaaagga gcaacgtgcc tggttcccgg ccgtaggccc gaaaggcctc ggtgaatcgg
      661 gcgatcggtc gcccgacaac aggtaatttc tcccagagac gacgaactct cccgccggtc
      721 cagtcgggtg cccaaaccaa taagagcaca atggtcccca gcacactgac caccagggtc
      781 aaccgacatg cgctttggac gatcgctggt cccgtggcat ggaagttcgt cagtaggatg
      841 gccccagacg ccacgagaag caacacgtat aatcccacga cacgatcgac gaagaccgag
      901 gccacagacg gggctcggtg ctcaggacaa tgtcgcgcga ccagtaccgc cttggctaga
      961 tctcccccca caatgcccat cggggccaga ttgagaaggt acccaacgaa actaaatcgg
     1021 agcgattcgc gaagactgat aggtaccccg actgaccgaa tcaatagcca ccagcgaatg
     1081 aatgtgatca tgacgctggc gaaagcacaa atgaaggcca ggaggaggat taaccaatta
     1141 tccacggcat ggcggacgag tgcccaaagt ttttccggct gaaagccatg ctcatcggag
     1201 aaaagcccct ccccgcggcg gacactatcc caaacaagcc acgccaggat agccaccgaa
     1261 gcggcgatct ttaataattg gacacacgtc cttttgaaac gcgaatgtgc tcgcataaaa
     1321 cacaagcccc ctgcggggcg aaacaatctt cttgttacag ctttgttgat gttgccgagc
     1381 agcctttacg gaagtctgtg tgcccgatcg gcaatctcgc gccggacccg ttccaaatcg
     1441 gcatccacca tcattttgac taattcaggg aaggagacct tcggagacca ctgaagcaca
     1501 cgccgggctt ttgaggcgtc gccgcgcagg gtataaacct ccgccgggcg aatcaatttc
     1561 ggatcaatct cgacataccg tttccaatcg agtccggcat agtcaaatgc tagttcgaca
     1621 agttcccgca cggagtgttt ttctccggtg gcgatcacat aatcatccgg cttcggctgt
     1681 tgaagcatca gccacatagc ctccacataa tcaccggcaa atccccaatc ccgcatggcg
     1741 tccagattgc cgagatagag tttatcttgg actcctaact tgattcgcgc gacagcgtcg
     1801 gtcaccttgc gggtaacgaa ttccttgcca cgccgcggcg actcgtgatt aaacagaatc
     1861 ccacagcatg cgaagacacc gaaactctcg cgatagttga ccgtgatcca atgcccatag
     1921 accttggcca cgccgtaagg gctgcgggga taaaatgggg tgttctcgtt ctgaggttcc
     1981 tcgcggaccg ccccaaacat ttcgctagaa ctggcctgat aaaagcgaat tgtcggatca
     2041 acgtggcgaa tggcctcaag catccgagtg acgcccaagg ccgtgaactc tcccgtcagc
     2101 aaaggctgtt gccaactggt gggcacaaag ctcatggccg ccaggttata tacctcatga
     2161 ggccgaacct ccgcgacaag tcgcaccagg gaaagttgat ccaagagatc ggcctgatgg
     2221 aggtgaagtt gatcccgaat atgctggatg cgttcgaagc cctccgtgct cgcccgacga
     2281 accatcccat gaacctcgta ccttttactc aacaggaact cggccaagta agaaccgtcc
     2341 tgtcctgtga tccccgtaat gagcgccctt ttggtcataa gcccaagatc ttttcaaaag
     2401 attgacagtt caatgattca gataattgac ccacaaccgc tgcgttgtcc ccagtttctc
     2461 tgggtgtgtt aatgtcagcg gatgttccac cacgtcccct atctttccat gataacctct
     2521 gagcggcacg cgatgttgtt tgacggagaa aagtctagtg ccaacccggc cgcgaattcc
     2581 ttcgatttaa ctcattgtgc aaaaccggca aactcagtcg aattgttccc ccatccgtgg
     2641 gttgccgttt ttgccgagag ggcggtagtt accgtttttg cctttttgcc gcctagcccg
     2701 agcttcctcg gaggcaatca caacgcggac gaacttagag cctatcccaa agttccctct
     2761 ccccctggga gacggttggc atgagggcaa gcgaccgttg gagtcgatcg agaaaaccgg
     2821 cagcgattgg agttactctc atcctcggcc cccctcccgc tgaacgggaa agggcagatc
     2881 gtgggatagg ctctttgcca atgacggagg tcgcttatcg tggacaaaac caaccagcgt
     2941 aatttgttca ctgtttctta gtttcggcga ggagattggg tctaaaggtc acggatggaa
     3001 ttgaagagcc gcacgtaaaa ggcgtcaatg gcctgggcgt tcaacgtggg cgtgatcttg
     3061 ttgccctcga ccacggttgt gtaaacgggg ccgagaacga tctccgcgta gcgtccgggt
     3121 gttaattgag agttcgatct gatttcgtta ttgaggatga aattgtcccg cccaccgttg
     3181 accacgatgc catttcgctg attgttgtat atcacgttat tcatgacgac attgaatcgg
     3241 tcaccatagt ctccgcccag accgaggcca ccaatgccgt aataaccatt tccataaagc
     3301 tcgttgccaa cggcccaaat tccgaaattg ttgaagcagt aatggaaacc gttgccgccg
     3361 ttgtaacggc tgacattgtt aatccacaaa cccccgatgg tcaaatcacc ggcaggatgc
     3421 aagcctttgc cgaggttgta ttctgtgagg cagttttcca cgcggatatc acgtccgcca
     3481 ctgactagga tcccctctcc ccaatcactg atgtcggcca tgccgtagcc gcttcctcga
     3541 acggtacaac ggcgaacaat actgtcggtt tcccacacca gactgatgcc gtctcggcgg
     3601 gagttttcca cggtgcagtt ttctagaagg ttgtcatgcc cctgctccag catgatccct
     3661 gtggccggag accttaccac ggttacgttc ttgatggtgg tgttggcggc gtaaaccaca
     3721 gacagggttg ccgggaggac gacccgccac cttctgtaat tctcccggag gttcccatcc
     3781 aatcgcacgt tctcgatctt ggcgttcaca gccggcgcgg taactgttcc gttgacggaa
     3841 accaacgaaa agacgttgac gacttcggcc ttgttcgtgg gcgaataccc tgcttccgga
     3901 atgggacggt cgagaatgag atcgtttccc tcgatgccga caatgacaaa tgtcttttcc
     3961 cgtgtatcga acgtggtgag ttgggagaca agagcaattt catcgcctac ccggaatcct
     4021 gccgtggatt cgacggtgat cctgcgatca ccaggccgag cctctgttgt tacgaaggca
     4081 ttaagcagcg gcgcgcgcct gagagtggtc ccgctgtcgc tcccccgcag tgtgacattc
     4141 gatcgtagca ccaaaccctg gcgaagttcg aatacacccg ctggcaagat cacgacacca
     4201 ccctcgtcgg gcagtgcgtc gatggcacgt tgaaagccca acgtggggct gcttgggtcg
     4261 aaatagtcga tcgcgtagat gacggttgag gactcttccc gattgatttt cacgcgcgga
     4321 gcagccccgt tcacctcgtc cgtggcatcc aaggagtagg cttcctgctt gaatcctcgt
     4381 gccgttacca ggatttgtgt ctctgctaga aagaactgtg ttgccgatga cgccacggtg
     4441 agctgatcct ttccgtcaga cccgtaccca aacacccggt cggttccgcc acccgtacta
     4501 tatgccacaa cgttggcgaa ggtttccaca cggttggaaa aaccggggcc ttccaatcgc
     4561 acatacaacg ggcggatggt taactcatca ttacctgaag aatcgtagag cttggccgtg
     4621 tcataaccgc cgctgcggga ataagcgtaa acggcgtcga aaaacttgac ccgggaaaaa
     4681 taggggtccg tcctgaacga agagaaatcc tgtcggccga cgaataggtc atcgcgcggg
     4741 gaatcgtaaa aatacgctgt atcaaagccg ccgtagcggg agtaaatgtg ggtgaaggca
     4801 aaaccataga ccctcacggt catgtccata ttgaacaggc tgacatcgcc ggcaaaggaa
     4861 aagacccagt cgtcttgggg ggagtcgtcg agttgcgcgg tatctgttcc acccgccgac
     4921 cggacaatcg ttgtccaaaa gttgaccgcg cggacattgg ttccccccgc cgtgagagta
     4981 atgtcgccca atcggccgct cagacgatca gatcctttcg tgcccaccag tgaggccatg
     5041 tcctcggctc ccggtgttcc gacaaaggtc accgattcaa agaatttggc gcggttataa
     5101 aaaccggcac cctgaaggat tgctaccccg tttttgtaag acgcttgatc cgcttgaggg
     5161 gaatcgtaaa aggtgatggc atcctgccca ccattccggg cataaacgtg aatagtttgc
     5221 ggtccgacga tggttgctga aaagcctccc ccagatatcg aaacacgctg aggaaatatg
     5281 acactatcat catcggcggg agagtcgttg acttctgcca ctgcggatgg actggcgtag
     5341 agggtaaggt tctcgatgcc ggaaacctca atggatcgtg acggcgtgct aacgaccacc
     5401 cttcccgggg tgtaaacaat gttttcgctg ttcaaacccg tgacaattgt ggcggagtcc
     5461 tcaccgagtc cggcgttaat gctcagccgc tgccaggcgg tggaaaactg gtaagtgatc
     5521 ctgtttaccg tgagccggct ggtaccgtcg aactcaaacg tgttgtcacc ggctgttcca
     5581 atgacagtta cggttgtccc ggagttttga acaaggttag caaggaacaa ttgttcccca
     5641 ctttgaacct gaccaagcca gacgaagtag gtcgatccgg cagtgacagg tgtgtccacc
     5701 ctgccgttac cccgagcaag ttccaccagt gcgttaccgc tgaccgcgta caccgtaaca
     5761 ccaagcgggc tgggatcagc cggtgacgga ccgcaaatcg cggtcaacgt cgccgtgttt
     5821 tgggcggtta gtcggaacca ctgccccgcc gaagacgaat ctgcaagggt cacctgggaa
     5881 tagtccacag ggcccagatc gaggaattgg ccgggcagcg gaagcgatgt caccgaaaga
     5941 agatgacgct gctcaagtga ctcaatcaca aggcgacgtg tcgccctgga cggccgccga
     6001 aacgacatga gacaagtctc ccaggtgcca ac
//
LOCUS       DAOSUT010000215         5952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6196_length_5952_cov_5.148041, whole genome shotgun sequence.
ACCESSION   DAOSUT010000215 DAOSUT010000000
VERSION     DAOSUT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_5952_cov_5.148041"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1597)
                     /locus_tag="PLD05_14375"
     CDS             complement(389..1597)
                     /locus_tag="PLD05_14375"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HOP78673.1"
                     /translation="MDSTLGTAAIQYASRGWHVFPLVPRDKAPLTDHGFYDASTDPAT
                     IREWWRHWPRANIGIRTGQASRLLVIDADGEEALAKLRDLSTRLGQLPRGPRVKTARG
                     WHGYFSTDSPVPSRKIEGVIDIKSEGGYVVAPPSVHPSGIVYTWQPPPNDAPPPFLPE
                     SWLQYLSNACYRENRESICYRENRESICYRENGGDSGGCASLRESNALDRVIEAAISE
                     TLPTGPGQRHRAIFELVRRLKAIPGIADTNAMDLLPIVRKWHRLALPVIRTKDFEETL
                     ADFLDGWERVRFRHGESPIEIALARAREAPPPPEAQTFEDPRLKLLVALCAELQRLQG
                     NAAFFLSCRAAGKAVEVDHMTASRWLKVLAKAGILQIEVQAERSTGRATRYRYIAKSP
                     CEANDIAIPS"
     gene            complement(1664..1972)
                     /locus_tag="PLD05_14380"
     CDS             complement(1664..1972)
                     /locus_tag="PLD05_14380"
                     /inference="COORDINATES: protein motif:HMM:NF019248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1580 domain-containing protein"
                     /protein_id="HOP78674.1"
                     /translation="MAIDLTREKLISFREAVELLPRRRRGRKVHVATLYRWATHGLHG
                     VRLETVQCGGTRVTTIEALQRFFRHLEGQRDDVKSPRTYAKRCRDSERAVEELAAEGI
                     "
     gene            complement(2128..2811)
                     /locus_tag="PLD05_14385"
     CDS             complement(2128..2811)
                     /locus_tag="PLD05_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78675.1"
                     /translation="MSESTNFSASKQIGYDAYPELIQLCLDLTHGIRMLRFWLYPSCN
                     VGEQSRRERLTHLVEAIRQKLELAQSRYEPAAVEPRKIVVGRRHFRDPIEAVLRLAQY
                     ALWSLPDFQDFRFNEVPEDRRNKVLNLVTPDYDSIDRLECELRCVRFHPQQEKQPLVL
                     TALQKEILKALDRRALKKEDLADRLKIDPARLYRPGGIKELIAQALVAHKWMVGYYRP
                     DRPPAECGD"
     gene            complement(2815..4044)
                     /locus_tag="PLD05_14390"
     CDS             complement(2815..4044)
                     /locus_tag="PLD05_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOP78676.1"
                     /translation="MAQRDRIPAYRLHRPSGQAVVRIDGKDFYLGRYGTVESRAEYDR
                     VIAEWLAAGRMVPATGCDLTIAELCLRYWRFAKSYYTVDGKPTGSLDRVKVAIRTLKD
                     TYAHTLAKDFGPLALQAIQQKLAASGRCRRYCNHIVDQIKRIFKWGVAQQLVPETVYR
                     AIQTVPGLRKGRSPAPERPPVRPVPDEVIEATLPYLPPVVADMVRLHRLIGARPAEVC
                     AMRPCDIDTSGPVWVYIPQHHKTEYRDHQRVIFIGPKAQEILRPYLLRPKDSFCFVPA
                     ESEKKRLIIRHRLRKTPLSCGNRPGSNRKRRPKKKPGEAYTTHAYYYAIRRAVAKANR
                     ERLRQHVANGGDAKDAPQIPYWTPNQLRHTAATEIRKLFGLEAVQAVLGHRNMLISEV
                     YAEKNLSLAREVARQIG"
     gene            complement(4162..4251)
                     /locus_tag="PLD05_14395"
     tRNA            complement(4162..4251)
                     /locus_tag="PLD05_14395"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4199..4201),aa:Phe,seq:gaa)
     gene            4905..5507
                     /locus_tag="PLD05_14400"
     CDS             4905..5507
                     /locus_tag="PLD05_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78677.1"
                     /translation="MTTFLVLILILIYIGVVLLLVFCGYFYIPYQAYRLDLFRQRLFE
                     IRDELFDKAADGVIDFKSPLYRKTRDIINGYIRFGHRYRCGYLISRALFFLVESRSRD
                     EDDITSFLMQLSQLDEQAQSAILLALVQSHAELIRYVHDRSLFARLSWAVLRRAKSFG
                     AVWSKLRRLFESPKYLKWVMELFRPLNQVGDVLPSHSAGW"
     gene            complement(5568..5756)
                     /locus_tag="PLD05_14405"
     CDS             complement(5568..5756)
                     /locus_tag="PLD05_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78678.1"
                     /translation="MEDKHHRPTDDQGFQDIPRKRSLELARHVAEIQRYQKENNLEDK
                     GDPPDEDNDSPQQRRERD"
     gene            complement(5728..>5952)
                     /locus_tag="PLD05_14410"
     CDS             complement(5728..>5952)
                     /locus_tag="PLD05_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78679.1"
                     /translation="LRPHCPPRQNFVDMGQSVIHRSVIGKHRFPEHYAGDGLLFERLG
                     QWAEVICVDEEATYYNALRDNDGRQTPPAD"
BASE COUNT         1176 a         1628 c         1734 g         1414 t
ORIGIN      
        1 ggactggcct tttctccagg cacgatgcga ttggccttca aatcgtgaac agtgccatgc
       61 gcggaaaccc tggaaaagac cgcatttttg ttttttgctg gatcacttcg cggaaaaaca
      121 ggtgcaaccc ggagtgcaac caaaatcgat gacgcattga aatgccggtt tgtaggccac
      181 cgctgaaagg tgagcagcgg atacagtgtc catcgccttc ggtcacatcc gcctatcgat
      241 cacgatcgca aagtcgatgt cacgttctcg tcacgctgat gtcacgctga atcgaaacct
      301 ttcaaaaagg tttggggtaa cgtttggggc aaggttgcag ggaacctttc ggggagccta
      361 ttcccgtgcg gatgggatcg ggatggggtc aggatgggat cgcgatgtcg ttggcctcgc
      421 atgggctttt cgcaatgtac cggtaccgcg tcgccctccc ggtggatcgc tcagcctgga
      481 cttcgatctg gagaatgccc gctttcgcaa gcactttcag ccagcgggac gctgtcatgt
      541 gatcgacctc cacggccttg ccagcagctc tacaggacag aaagaaggcg gcatttccct
      601 ggagcctttg cagctccgca cacaaagcca cgagtaattt cagccttgga tcctcgaagg
      661 tttgagcttc aggcgggggt ggggcctctc tggcccgggc cagtgcgatt tctattggac
      721 tttccccgtg gcggaacctt accctttccc agccgtccag gaagtcggca agtgtttcct
      781 cgaagtcctt cgttcggatc accggcaggg ccaatcggtg ccatttgcga acaattggca
      841 acaggtccat ggcgttcgtg tctgcgatgc ccggaatggc cttcagtcgc ctcaccagct
      901 cgaagatcgc ccggtgccgt tgtccgggtc cggtaggtag tgtctcgctg atagcagcct
      961 cgatcacgcg atccaatgcg tttgattcgc gaagcgatgc gcaaccgcct gaatctcctc
     1021 cgttctctct gtaacatata ctctctctgt tctctctgta acatatactc tctctgttct
     1081 ctctgtaaca tgcattagat agatattgca accagctttc tggcaaaaat ggcggtggcg
     1141 cgtcgtttgg cggcggttgc caagtgtaaa cgattcctga cgggtgcacg cttggtggtg
     1201 ccacaacgta acccccctcg gacttgatgt cgatgacgcc ctcaattttg cggcttggga
     1261 caggggaatc ggttgaaaag tagccatgcc aaccccgggc cgttttcacg cgcgggcctc
     1321 gcgggagttg gcccaatctc gtggacaggt cgcggagctt ggctagtgct tcctcgccgt
     1381 ccgcatcgat gaccaagagc ctcgacgcct ggcccgtccg gatgccgatg ttcgcccgcg
     1441 gccaatgtcg ccaccactcc cggatcgtgg cggggtcggt actcgcatca tagaacccgt
     1501 gatcagtcaa cggggctttg tcgcggggaa ccagcggaaa gacgtgccaa ccgcgggatg
     1561 cgtattgaat cgccgctgtg cccagcgtag aatccatgcg agactccttt attaggcgac
     1621 ggtgtgacct cgcgcggcaa ccgtcgcctt ttttctcgcc cgatcaaatc ccttctgcgg
     1681 ctagttcctc aacggcccgc tccgagtcac ggcaccgttt cgcatacgtg cggggtgatt
     1741 tgacatcgtc acgctgccct tccagatggc ggaagaatcg ttgcagcgcc tcgatggtgg
     1801 tcacccgggt gccgccgcat tgcacagtct ccagccgaac cccgtgcagc ccgtgcgtgg
     1861 cccagcgata gagcgtcgcg acgtggactt tccgccccct tcgtcgccgg ggaagcaact
     1921 cgacggcctc gcggaacgag atgagttttt ctcgggttag atcgatcgcc atacaaacct
     1981 ccattaacaa gtgatccttt cacttgcgat tgtaggcgac caaatgtgtt tgtcagggac
     2041 tatttggtga cgagttagtg gcgctttggt gactcgatgg tgactggaag acgggccgta
     2101 cctgaagagg tctacggatg tgatagttca atcgccacat tctgcgggag ggcggtcggg
     2161 ccggtaataa ccgaccatcc atttgtgggc tacaagagcc tgcgcgatga gttccttgat
     2221 tcctccgggc cgataaagtc gggctgggtc gatcttcagg cgatcggcga ggtcttcctt
     2281 ttttaacgcg cgccggtcga gcgccttgag aatctctttt tgaagggcgg tcaaaacgag
     2341 cggttgtttc tcttgctggg ggtggaagcg aacgcaccgt aattcgcatt ccaggcggtc
     2401 tatcgagtcg tagtcggggg tcaccagatt caagaccttg ttgcgtcgat cttccggtac
     2461 ctcattaaag cgaaaatctt ggaagtctgg caggctccac agagcgtact gagcaagccg
     2521 caagacagct tcaattggat cccgaaagtg tcgccgtccg accacaattt tccgcggctc
     2581 gaccgccgcc ggttcgtagc gtgactgtgc cagttccaat ttctgacgaa tagcctctac
     2641 aagatgggtc agtctttccc gtctagattg ttcgcccaca ttgcaactcg gatatagcca
     2701 gaaacgcagc atccttattc cgtgggtcag gtctaggcag agttgtatca gttcaggata
     2761 ggcgtcatag ccaatttgtt tagacgcgga aaagtttgtg gattcgctca tatgtcaccc
     2821 aatttgtcgc gcgacctcgc gggccaggga taggttcttt tcggcgtaga cctcgctgat
     2881 cagcatgttt cgatgtccaa ggacagcctg gacggcctcc aggccgaaca gtttgcgaat
     2941 ttccgtcgcc gccgtgtggc ggagttgatt gggcgtccag tagggaatct gcggggcatc
     3001 cttggcatcg ccgccgttgg cgacgtgttg gcggagtcgt tcccgattgg ccttggccac
     3061 cgcccgccgg atggcgtagt agtaggcgtg ggttgtgtac gcctcgccgg gttttttctt
     3121 cggtcggcgt ttgcgattgg agccgggccg gttgccgcaa ctgagcgggg ttttcctcag
     3181 gcgatgacgg atgatgagcc gctttttctc gctctccgcg ggaacgaaac agaacgaatc
     3241 ctttggcctc agcaggtatg ggcgaagaat ttcctgggcc tttggaccga tgaagatgac
     3301 ccgctgatgg tcccgatatt ccgttttgtg atgctgcggg atgtagaccc acaccgggcc
     3361 ggacgtatcg atgtcgcaag gccgcatggc gcagacttca gcggggcgtg ctccgatgag
     3421 gcggtggagg cggaccatat cggccaccac cgggggcagg taggggagcg tggcttcgat
     3481 cacctcatcg gggacgggcc ggacgggagg gcgttcgggg gccggggagc gtcccttacg
     3541 gaggccggga acggtctgga tcgcccgata aacggtttca gggaccagtt gctgggcgac
     3601 gccccatttg aagatgcgtt tgatctggtc aacgatatgg ttgcagtatc gccgacagcg
     3661 gccggatgcg gccagtttct gctgaatcgc ctggagggcg aggggaccga agtctttggc
     3721 gagggtgtgg gcgtaggtgt ccttcagcgt gcggatggcg actttgaccc ggtccaggga
     3781 accggtgggc ttgccgtcca ccgtgtagta gctcttggcg aagcgccagt agcgaagaca
     3841 gagttctgcg atggtaagat cacatcccgt cgctgggacc atccgacccg ccgcaagcca
     3901 ctctgcgatg acccgatcgt actccgcgcg actttcgacg gtcccgtacc tacccagata
     3961 aaagtctttc ccgtcaattc tgaccaccgc ctgcccgctc ggcctatgaa gccgatacgc
     4021 cggaatccgg tcacgttgcg ccatgcgtgc accctcaatt ccgaaaagtg gtagtctacc
     4081 actttttttg gaaccgaacg ccgcacggcc gaccgccggc cggtatccgt aacccatgga
     4141 ttcagcgtca cttacgcaca gtggacaggg gcgggatcga accgccgaca cctggatttt
     4201 cagtccaggt gtcgccatcg ctgatttgcg ggctccagtg cggaccgctc cgggcgagcg
     4261 cttttgcaga gtaagacaga aacgccgacc gcgaatcgat gataggcgtc ttaaggtggg
     4321 cccctagcct acaagaaaga gggagaaata cgggatcaga ttgcatcagg agagttgctt
     4381 atcggtcaca ggaaagctaa acttgcctgt gccgcatacc ggggtcaatc acaatatccc
     4441 gcggtctggg cattttcgga tgatcgctaa cattcacggt tagcgtgaag gcactgattt
     4501 gcgggccaac ctatgagcga ctctgcgcaa gaatctccgg gttcaattcc gagagacttg
     4561 gaactgcgaa agattgagct caagtacgaa ttctggggta aaatagcacc cctctccata
     4621 aaatggggcg ccttgctcat cttgggtttg gttcacaaag aagttgcggt agtgctttgc
     4681 aagattctgt tggcaccggc aaattttttt atcggtgtga ttatcctcgc cctatccgcg
     4741 ggactgactc tggcaatcgt atgggcgatg aaagaacgag cgctccgacg acgaaagacc
     4801 gaatatttcc aatctcggat tcgttcccta gagcaaaggc tagacccgga gcgtacgtca
     4861 agtaagttga ctcctggcgg cgacacaaac ccggaggacg caaaatgacc acattcctag
     4921 tcctaatctt gatcttgatc tacattggcg tggtgctgct gcttgtcttc tgcggatact
     4981 tttatattcc ctatcaggct tatcgtcttg acctttttcg ccaacgtctt tttgaaattc
     5041 gggatgagct atttgataag gctgccgacg gagtcatcga ttttaagtca cccctgtacc
     5101 ggaaaacccg ggatatcatc aacggatata ttcgttttgg gcatcgttat cggtgcggct
     5161 atttgatctc tcgagcattg ttttttctcg ttgagtcacg atctagggac gaggatgata
     5221 ttacgtcttt cctgatgcag ctttcgcaat tagacgagca agcgcagtcg gccatccttc
     5281 tcgccttggt gcaatcccac gcggaactca ttcgatacgt tcatgaccga tcgttgtttg
     5341 cgcgcttgtc atgggcggtg ctgagacgcg cgaagagctt tggggcggtc tggagtaagc
     5401 ttcgccgact ctttgaatca ccgaagtact taaaatgggt aatggagctt ttcaggccat
     5461 taaaccaggt tggtgacgtg ttgcccagtc atagcgcggg ttggtaaccc cctcagacgc
     5521 agttcaaagt gattaaacca gatagcggca gaatcgaggg gattgattca atcacgctcc
     5581 cgccgttgtt gcggagaatc attatcctcg tcgggcgggt cgcccttgtc ttcaaggttg
     5641 ttttctttct ggtatcgttg aatctcggcc acgtggcggg ccagttctag cgaccttttc
     5701 cgcgggatat cctggaaacc ttgatcgtca gtcggccggt ggtgtttgtc ttccatcgtt
     5761 gtccctcaac gcgttatagt aggtcgcttc ttcatctacg catatgacct cggcccattg
     5821 cccgagtctc tcaaacaata gtccatcacc ggcgtagtgc tcggggaagc ggtgcttccc
     5881 gatcaccgac cggtggataa cgctctgacc catatcgaca aaattctgcc gcggcgggca
     5941 atggggccgc ag
//
LOCUS       DAOSUT010000216         5828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6329_length_5828_cov_10.083839, whole genome shotgun sequence.
ACCESSION   DAOSUT010000216 DAOSUT010000000
VERSION     DAOSUT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6329_length_5828_cov_10.083839"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLD05_14415"
     CDS             <1..872
                     /locus_tag="PLD05_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78680.1"
                     /translation="SQCSNNLKQIGLAIHNFQDVYGRLPTGARDGDHRVPDALTACCN
                     SRTRYGWSFWYHILPFIEQRGLYDLATDADDPPTPGSGQNSKENIVAQKFVTTYYCPS
                     RRLPEPYGSGKFYRSDYAGNAGQRGEGDIRDTPSGGLRGPIIQTDRNRTRIELIRDGS
                     SNTILVAEKALHPKSFGSDGGDNERWNNAGWDEDIIRFGAGRLNNGTEYGLPPIPDNT
                     APYNPGSGWTTIKDLGGRTWGQWHPFFGSSHPGGMNACMVDGGVRFISFTVNADTFRR
                     LSLTDDGETVDNF"
     gene            1126..1308
                     /locus_tag="PLD05_14420"
     CDS             1126..1308
                     /locus_tag="PLD05_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78681.1"
                     /translation="MRQFLGKWLSVALFFVCLGVCSIQPGCKRTPPEPPALTEEVKSA
                     IQAEDQEIFEQEKAQR"
     gene            1572..2630
                     /gene="lpxD"
                     /locus_tag="PLD05_14425"
     CDS             1572..2630
                     /gene="lpxD"
                     /locus_tag="PLD05_14425"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOP78682.1"
                     /translation="MPITLGELSDLVGGELDGPAHLVICQAKPLGEAGPTDITLIEKP
                     EKVAKISPGQIGAAVVPRSVTRANFPVIRVDDVHKAFGLIVRWFRPPRKLTRKGIHHQ
                     AIISPSAKIADDVEIGPFSVIGDDVEIGSGTTIYGGVHILSGCKIGRDVTIFPNVVLY
                     EDTIVGDRCIIHAGAVIGAYGFGYRLESGRYVLQAQLGNVILEDEVEIGANTTVDRGT
                     YGSTRIGQGSKIDNLVVIAHNCQIGRHNILCAQVGIAGSTTTGDYVVMAGQVGVRDHV
                     HIGERAVLGAMSGVINDIPPGARFVGIPATPEREQMLKQAAFTKLPEMRKQLKELQEV
                     VRQLTARLESLESSREAA"
     gene            2644..3579
                     /gene="lpxI"
                     /locus_tag="PLD05_14430"
     CDS             2644..3579
                     /gene="lpxI"
                     /locus_tag="PLD05_14430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOP78683.1"
                     /translation="MSLPLNDLIDPVSQSRHVSQKPLGLIAGWGRYPVLLAQRLSEQG
                     RPVYCLAVRDHADPALADYAERIVWLGLGQLGRAIRLFRRWGVEEATLVGKIHKVTLL
                     QPWMVFHHWPDWVGFRTFAPHFLHHVRDNRDDTLLTTVCNAFELAGIRIRPATDYLPE
                     ILISPGQITPQAPTPRQWADICFGWRVAKEMGRLDIGQTVVVKDQTVLAVEAIEGTDP
                     CLRRAGELCPVGGFTVVKVAKPQQDMRFDVPTVGVRTLENIASGKGSVLAVEAGKTIF
                     LDPDAVLSIALRHRIAIVAVTAEDIAAHAKDALPA"
     gene            complement(3590..5821)
                     /locus_tag="PLD05_14435"
     CDS             complement(3590..5821)
                     /locus_tag="PLD05_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78684.1"
                     /translation="MRLTLRTLLAYLDNILDPEDAAELRRKIEESDVAKAVLRRIREL
                     LRKERLGAPDVNDRQESLDPNTVAEYLDNTLPAERVADFEKVCLESDIELAEVAACHQ
                     ILAMILGEPAEVDPKTREKMYRLPQTSALSEKPLEKIVKELVAEPPAEEAARAASTKP
                     ETGKTPPAEPVTFVSPTPHVRSWLLGLMVLLVVGAIGVGALALYKTLLPAPAGLPGGE
                     VAQQTQPVKPHVDRGKSGVPDGGAPAKEPRGSVEGATPESPPGTPEAQQQTAPSTETA
                     PTSSPPAPPKADAAEAVAQKPSEQPPKTPEDESMVPPMEESPAPPVAEQAPDALAAVT
                     PQRGVPAPGEMLPSAPEIREVAPLPPEPIGRIVEDPKIPPILFRVHPDGALLHRVSLE
                     EPLRTSEVFFVPPKYRTTIRLADSVDVLCVGPVFWELLPMDANGQLGIGITFGQMILT
                     SRQADPTTLRLKCGNIEGRIEFGDAQSQVAIAVSRQPVCPGDPETQPVPWIVDMFSLG
                     GTARWFDAEHPRPLVLRAPVQMRLSEAELAATPMTDQPEWIKPRVTTATILEERAMAV
                     LEQALRDREKDPLLVLREQSANRQREVAWLAQRALAFAGEADVIWRVLEDLEYRPVWT
                     EAVAVLRELVRISPRHAAAVRAAAEKTLGMSGTLAYTLLWKYPDTGITKSQAEELVQQ
                     LEHNNLAVRVVAFWTLRHITGMTLNYEPHSLPNDRTRAVQLWRERLTKGSVRGVADER
                     PNL"
BASE COUNT         1187 a         1646 c         1732 g         1263 t
ORIGIN      
        1 gatcgcagtg ctcgaacaat ctcaagcaga tcggcttagc tatccacaat tttcaggatg
       61 tgtatgggcg gcttcccacg ggtgcccgcg atggtgacca ccgcgtcccc gacgctctaa
      121 ctgcatgctg caattcgcgg acgcgttacg gctggagttt ttggtatcac atcctgccgt
      181 ttatcgaaca gcggggactc tatgatttgg cgaccgatgc ggatgatccc ccgacgccgg
      241 gatctggcca aaactccaaa gagaatatcg tggcccaaaa gtttgtgacc acctattatt
      301 gcccttcgcg acgccttccc gaaccctacg gtagcggaaa gttttaccgg tcggactatg
      361 ccgggaatgc tggacagcga ggcgaaggcg atatccggga cacgccaagc ggtggtctgc
      421 gtggcccgat tattcagacc gatcgcaata ggacgcggat cgaactaatc agggatggtt
      481 cttccaacac gattttggtg gctgaaaagg ctctccatcc taaatcattt gggtcggatg
      541 gtggcgacaa cgaacgttgg aacaacgccg gctgggatga ggatattatt cgctttggcg
      601 ccggacgact taacaacggc accgaatatg ggcttccgcc aatcccagat aacacggcgc
      661 cgtacaatcc gggcagtggc tggacaacga tcaaagactt gggtgggcgg acatgggggc
      721 agtggcaccc cttcttcgga tcgtcccatc ccgggggtat gaacgcctgt atggtagatg
      781 gtggtgtccg cttcatttct ttcaccgtca acgccgatac gtttcggcgg ctaagtctca
      841 ctgacgacgg tgaaaccgtt gacaactttt gaaaagcaga gtctctgtta cccgccatgc
      901 caaggatcgc ccgcagcgcg tcacccgatg ttcccgcgcg agtcgcttta accacctgtg
      961 ctgcgggcga tctcttggca gaaaacacga ttttgacggc gcccctaatg cccaccgctg
     1021 aagaaggttc ttggccgatg cggcggtaac ctactgaatt ctgacgttat tggcaatatt
     1081 caccttacta gctcacagca tgctcgattg tgaggagacg aaagagtgag gcaatttctt
     1141 gggaagtggc tgtctgtcgc gttgtttttt gtttgccttg gtgtgtgttc catccagcct
     1201 ggctgcaagc ggacacctcc cgagcctcct gccctgacgg aagaggtcaa atcggccatt
     1261 caggctgagg accaagagat cttcgagcag gagaaggccc agaggtaatt tctcatttcc
     1321 ggcgacggcc tcccccagta atcatcccgg tgtggcgtgg cccctacgga cgatcattca
     1381 agggggcgtg aaggttcttt ctgcggttcc ccattttggg gcaaggagaa tattggcatg
     1441 cgggcattct ggcagctagc tttgatcttg catggaggcc ggggtaaagg ttagcataat
     1501 atccacatgc tggcccgatt gccgacattg agaaggatga tttgtcgccc attaatggaa
     1561 aggaatcccg catgccgatc actctggggg agctttcgga cctagttggg ggggaactcg
     1621 atgggccggc tcatctggtg atttgccagg cgaagccgtt gggagaggcc gggccaacgg
     1681 atattacctt aatcgaaaaa ccagagaagg tcgccaagat ttcgccaggt caaataggcg
     1741 ccgccgtggt tccccgcagt gttacgcggg cgaactttcc cgtcattagg gtggatgacg
     1801 ttcataaggc ctttggcctg attgtccgct ggttccggcc acctcgaaag ctcacacgaa
     1861 aagggattca ccaccaggcg atcatcagcc cgtccgccaa gattgccgac gacgtggaaa
     1921 tcggtccatt ctccgtcatt ggcgatgacg ttgaaatcgg ttcgggaacg accatctatg
     1981 gtggggtgca tattctgtcg ggctgcaaga tcggtcgcga tgtgacgatc tttcctaacg
     2041 tcgttctcta cgaagacaca attgttggcg accggtgcat catccacgcg ggggccgtca
     2101 tcggggccta cggcttcggt taccgtttgg agtccgggcg atatgtgctc caggcccaat
     2161 tgggcaatgt gatccttgaa gatgaagtgg agatcggggc gaatacgacg gtcgatcgcg
     2221 gaacttatgg ttcaacacgg atcggccagg ggagtaagat cgataacctc gtggtgattg
     2281 cgcacaactg ccaaatcgga cggcacaaca tcctatgtgc tcaggtgggg atcgccggca
     2341 gcacgaccac aggggactac gtggtgatgg ccggacaagt cggtgtgcgg gaccatgtgc
     2401 acattggaga gcgcgccgtg ctgggcgcca tgtcgggggt gatcaacgat ataccgccgg
     2461 gcgcccgctt cgtgggcatt cctgcaacgc ccgagcggga gcaaatgctc aagcaggcgg
     2521 ccttcaccaa acttccggaa atgcgaaaac aactcaaaga attgcaggaa gttgtacgtc
     2581 agctgaccgc ccgcctggaa tcgttggaat cctcgcggga agccgcgtga caggtgacgt
     2641 ctcatgagtc ttcccctcaa cgatcttatt gatcccgtgt cgcaatcgag gcatgtttcg
     2701 cagaaaccct tgggattgat tgcaggctgg ggacgctacc ccgttcttct ggcccagcga
     2761 ctgtccgagc aggggcgacc ggtctattgc ctggccgttc gggatcacgc tgaccctgcc
     2821 cttgccgatt atgctgaacg aatcgtctgg ctaggactgg gccaactggg ccgtgcgatt
     2881 cgcctattcc gacgttgggg tgtcgaagaa gcgacgctgg tgggcaaaat ccacaaggtg
     2941 acacttctcc aaccatggat ggtcttccat cactggcctg attgggtagg gtttcgcacg
     3001 tttgccccac acttccttca ccatgtccgc gacaatcggg acgatacgct actgacgaca
     3061 gtatgtaacg cctttgaact ggcaggtatc cgcattcgac cggctacgga ttacctcccc
     3121 gaaatcctca tttcgcccgg tcagatcaca ccccaagcac caacgccgcg gcaatgggcc
     3181 gatatctgct tcggctggcg ggtagccaag gaaatggggc gcctcgatat cggtcagact
     3241 gtggtggtca aggaccaaac cgtgttggcc gtggaagcca tcgaaggcac ggatccttgt
     3301 cttcggcggg ccggtgagct ttgtcctgtg gggggattta ccgtcgtcaa ggtggccaag
     3361 ccgcagcaag acatgcgatt tgatgttccc acagtgggcg tccgcacctt ggaaaatatt
     3421 gcctcaggaa aaggttccgt attggccgtt gaggcgggaa aaacgatctt tctcgatccc
     3481 gatgcggtcc tgtccattgc tttgcggcat cggatcgcca ttgtagccgt caccgccgag
     3541 gacattgcgg ctcatgccaa agacgcgctc cccgcttgag tgacgccgat cacaggttgg
     3601 gccgctcgtc tgcaacacct cgcacagagc ctttcgttag ccgttctcgc cataattgca
     3661 cggccctagt gcgatcgttt ggaagggaat gcggttcgta gtttagcgtc attcctgtga
     3721 tatgccgaag cgtccaaaaa gcgacgacgc gaacggccaa gttattgtgt tcaagctgct
     3781 gcaccaattc ttccgcttgg ctcttggtga tcccggtgtc gggatacttc cataagagcg
     3841 tgtaggcaag cgtgccgctc atgcccaaag ttttttcggc ggcagcccgc accgcagccg
     3901 cgtgccgggg cgatatgcgg accaattccc tcagaacggc tacggcctcc gtccatacgg
     3961 gtcggtactc aagatcctcc aaaacccgcc agatgacatc cgcttcgccc gcaaaagcga
     4021 gggctcgctg ggcgagccac gccacttccc gctggcgatt agcggattgt tcccgaagca
     4081 caagtagtgg gtccttttcc cgatctcgca gggcctgttc taaaaccgcc atggcccgct
     4141 cttcgaggat ggtcgccgtg gtgacgcgcg gcttgatcca ttcgggttga tcggtcatcg
     4201 gggtcgcggc caattccgct tcgctaagtc gcatttgaac cggggcacga agcacaaggg
     4261 gccggggatg ctccgcgtca aaccaccgcg ccgtgccacc gagcgaaaac atatcgacga
     4321 tccagggcac cggctgggtc tcggggtcgc cggggcacac aggctgtcgg gataccgcaa
     4381 tggccacctg ggactgcgcg tcgccaaatt cgatacggcc ttcgatattc ccgcatttca
     4441 gccgaagcgt ggtgggatcg gcctgccgcg atgtcaaaat catctgccca aaggtgatgc
     4501 caattcccag ttgcccgttg gcatccatgg gcaaaagttc ccaaaagact ggtccgacac
     4561 aaaggacgtc cacgctgtcc gccaaacgga tggtagtgcg gtacttgggt gggacaaaaa
     4621 acacttcact agtccgtaag ggttcttcca gacttacacg atgaagtaac gcaccatctg
     4681 gatgcacccg aaacaagatc ggggggatct tgggatcctc aacgatccgc ccgatcggtt
     4741 ctggaggcag tggggctacc tcgcggattt cgggggccga agggagcatt tcccccgggg
     4801 caggaacacc ccgctgggga gtgacagccg ctaaggcatc gggggcttgt tctgccacag
     4861 gcggcgccgg ggactcttcc atgggtggca ccatgctttc atcctcggga gttttgggcg
     4921 gttgttcaga tggtttctgc gccacggctt ctgctgcatc tgcctttggg ggcgccggag
     4981 gtgacgaggt tggagccgtt tcggtagacg gggctgtctg ttgctgggct tcaggtgtgc
     5041 cgggaggact ttcgggggtt gctccctcga ctgagccccg cggttctttc gctggtgcgc
     5101 caccatcggg aacgccactc ttgccacgat caacatgggg ctttacgggc tgcgtttgtt
     5161 gggccacttc tccacctggc aagcccgccg gcgcgggaag cagtgtctta taaagggcca
     5221 gggctccaac cccgatcgca ccaacgacga gcaagaccat caagccaagc aaccacgaac
     5281 ggacatgagg cgttggcgag acgaacgtta cgggttccgc gggaggtgtc ttccccgtct
     5341 ccggttttgt tgaagccgcc cgtgccgcct cttcagcggg aggctctgcc actaattcct
     5401 taacgatttt ctctagcggc ttttcagaaa gggcagaagt ttgcggaaga cgatacatct
     5461 tctcccgtgt cttaggatcg acctcggcgg gctcacccag gatcattgcc agaatctgat
     5521 ggcaggcagc gacctcggcc aattcaatgt cggattccag acagaccttt tcaaaatcgg
     5581 cgacccgctc ggcgggcagg gtgttgtcga gatactcggc caccgtattg ggatcaaggc
     5641 tctcttgccg gtcgttaaca tcgggcgcac ccaggcgctc cttgcgcagg agctcccgaa
     5701 tccggcgtag cacggccttg gcgacatcgc tctcctcgat cttacgccgc aattctgccg
     5761 cgtcctcggg atcgagaata ttgtccaaat aagccaaaag cgtccgcagt gttagccgca
     5821 tggttcag
//
LOCUS       DAOSUT010000217         5612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6552_length_5612_cov_9.125427, whole genome shotgun sequence.
ACCESSION   DAOSUT010000217 DAOSUT010000000
VERSION     DAOSUT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6552_length_5612_cov_9.125427"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLD05_14440"
     CDS             <1..364
                     /locus_tag="PLD05_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78685.1"
                     /translation="DANMEIRRASGVFVYGLKGEYNRPIVKAIDAENFAIFGYGGNAA
                     AYPGTALFDIENCRNYRLTLLVDSPRYPGSGSPDHFAGEGVDPRLWHMVIEKQGDREF
                     RTPPLDRPTLVARGDLLR"
     gene            447..2903
                     /locus_tag="PLD05_14445"
     CDS             447..2903
                     /locus_tag="PLD05_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOP78686.1"
                     /translation="MNRFGTCFLAVVTTFGLCGWAFAESSPAVREIVLEEIAYTDEQA
                     AQQAWRASEGTPPIRLIKVDGGQALEIGLPFARHADLPRSIHDREVKLDLSRPGEFIL
                     ELEIDRPEAVGHLSLYFRSGAGWYAGSGSVSSVGKQLVRFVKAEFRSEGKPAGWGQID
                     GIRISFWRGASVDAQARIYRLTALEHPVALLVPTAVEGHEQSELRTIREAAERVGRLI
                     TDLGLGFDTLDDRNPSAEVWAEKAIIIVPYAPRLSPTAASALAEAIRRGTKVILFYQA
                     PQEVLTLLGVGKTRWMAREYESQFAEVRFDRSILPDLPVRMRQSSWNITEPIPTVAST
                     RVIGEWFDAQGKPTGRAAMILSPHGAFFSHILLGGDAENQRVMLAAVMGALRPDLKPV
                     ILRGLLAKVTPVGHLQNLDELREWLKKHAPDTTSAPWQLLQEAESLLSQIDVKDATRP
                     EQISLAALEDIRKKIVTAYLMAQPSPSREGRAYWNHTGLGAYPGDWERTARELAEAGF
                     NMVLPNMLWGGVAHYASDLLPRSASFEKYGDQIAQCVEACHRHGIEVHVWKVNFNLST
                     APNSFVEQMRASGRTQVTRRGEPTRWLCPSHPENQKLECETMLEVVKKYGVDGIHFDY
                     IRYPDGDNCYCDGCRQRFEKDTGLRVTSWPDDCYSGSLRAAYRNWRCEQITRVVQMVA
                     QEAKRLRVDIKISAAVFAEYPACRESVGQDWVSWVKAGYLDFICPMDYTEDDRRFERL
                     VKTQAELIGGRIPMYPGIGAYRLDGPDRVVGQISIARQIGVPGFTIFDLNEQSPQTIF
                     PAVRLGATKEKAIPAHRHQP"
     gene            complement(3001..4173)
                     /locus_tag="PLD05_14450"
     CDS             complement(3001..4173)
                     /locus_tag="PLD05_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOP78687.1"
                     /translation="MTSSRIYLDNAATTWPKPEAVYQAVDEYQRRIGCSPGRSTYADA
                     LAADRVVLEARLAVARWLRVGQADHVLFAFNGTDALNMALRGMIRPGDHVVTTIADHN
                     SVLRPLRWLAEHYGVRVDYVACDPEGLVELDALRSKLNRATRLVAVNHGSNVTGTLQR
                     LEAIVDAVRRHSSAMVLVDAAQSLGHVPLFPGEIGIDLLAAPGHKGLLGPGGTGILYV
                     RPGLEEELVPLRFGGTGSRSDEDRQPDEWPDRYEPGNPNTPALAGLIKGIEYLEQRGL
                     DDLRAHEVTLTQRLIDGLHAIPGVEVYGPKNAADRVGVVSINVKGLSPHELAAILDGQ
                     FHIQVRAGIHCAPLIHRRLGTLERGGAVRFSLGPFNTLEHVEAAIAAVRQIAQHFT"
     gene            complement(4265..>5612)
                     /gene="trkA"
                     /locus_tag="PLD05_14455"
     CDS             complement(4265..>5612)
                     /gene="trkA"
                     /locus_tag="PLD05_14455"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOP78688.1"
                     /translation="RMRILILGAGTIGASIAEFLCQTHRVSLIDRDQEAVDRVNETMD
                     VRALCGSAADAVLLFQAGATDADLCLAVTNSDEVNLVGASVAKAMGTRRAIARVYSPN
                     LLDTSTFDYRRHFKLDYLVSLERLAALDLARVVRSPGALSIETFAVADLEMQEIVVQP
                     GAPAIDIPLRELRVGRHVRVGVISRGDKVFIAGPEDAVAVNDRIALVGKREDIDAVKS
                     LFQTEPPRRQGVVIAGGGETGFHLARLLEGPRFNVVLMESDHERCEFLASHLREVTVV
                     CCDVRSRAALEEERVGSADFFVACTGDDENNILACVEAKELGAQKVAAVVARSDYRYI
                     LTKLGVDHVTTPPDVVRSEVEAHLTTGVVIAKKPFAPGSKVLVMELEVLPNTLITQHV
                     LARVGLPRQCLIAALIREDFAILPGADDRIKPGDTVVALVHESAVESFLTFFEPAE"
BASE COUNT         1218 a         1580 c         1632 g         1182 t
ORIGIN      
        1 tgatgccaat atggaaatcc gcagggcctc cggtgttttt gtgtatggtc ttaaggggga
       61 gtacaatcgc cccattgtga aggccattga tgcggagaac tttgccatct ttggatacgg
      121 gggtaatgcg gcggcctatc ccggcactgc cctgtttgac attgagaact gccgtaatta
      181 ccgactgaca ctccttgtgg atagcccacg ctacccggga agcggctcgc cggaccactt
      241 cgccggagag ggtgttgatc ctcggctttg gcacatggtg atagagaaac agggcgacag
      301 ggaatttcgc actccacccc ttgatcggcc gaccttggtg gctcgcggag acctccttcg
      361 ttaggcattg caaagatagg gaatgttgcg agataggact aagaacgatc aaagtgatgg
      421 gaaacgaacc acaaggagtt tcggcgatga atcggtttgg tacgtgcttt ttggccgttg
      481 tcacaacctt tggattatgc ggatgggcgt ttgcggaaag ttcgccagcg gtgcgagaaa
      541 tagtcctgga agagatcgca tacaccgatg agcaagctgc ccagcaagcg tggcgagcct
      601 ctgaagggac gccgccaatc cggttgatta aggtggacgg aggccaggcc ttagaaattg
      661 gcttgccgtt tgcccgtcac gcggatttgc cccgcagtat tcatgaccgc gaggtgaagc
      721 tcgacctttc ccgccccggc gagtttattt tggaactgga aatcgaccgc ccggaggccg
      781 tcggacatct atcgctctat tttcgtagcg gtgcgggatg gtatgccggt tcgggatcgg
      841 tcagcagcgt gggcaagcag ttggtacggt ttgtgaaggc ggaatttcgc tctgaaggaa
      901 aacccgctgg gtgggggcag atcgatggga ttcgcatctc attttggcga ggtgcctccg
      961 tggatgccca agcgagaatt taccgtctga ccgccctgga gcaccccgtc gccctacttg
     1021 ttcccacggc cgtcgaggga cacgagcaat ccgaattgcg gaccatccgg gaagctgccg
     1081 agcgtgttgg gcgattaatc accgacctgg gattgggctt tgatacactc gacgatcgaa
     1141 acccaagcgc cgaggtttgg gccgaaaagg ccatcatcat cgtgccctat gccccacgtt
     1201 tatcgcccac tgccgcatcg gccttagcag aagcaattcg tcgcggcaca aaagtcatct
     1261 tgttctatca agcgccacaa gaggtgttga ctctccttgg ggtgggcaaa acccgctgga
     1321 tggcccgcga atacgagtcc caatttgcgg aagtccgttt tgatcgcagt atcttgcccg
     1381 atttgcctgt tcggatgcgg cagtcgtcat ggaacatcac cgaacctatt cccacggttg
     1441 caagcacccg tgtgatcggc gagtggttcg atgcacaagg taagcccacc ggtcgggccg
     1501 ccatgatcct ctcgccccac ggagcgtttt tcagccacat cttgctggga ggtgatgctg
     1561 aaaatcagcg cgtgatgttg gccgccgtga tgggggcgtt aaggccggat ctcaagcctg
     1621 tcattctgcg tggcctgctg gccaaagtca ccccggtcgg tcacttgcaa aacctcgatg
     1681 aactgcgaga gtggctcaag aaacacgctc cggacacaac gagcgcccct tggcagttgc
     1741 ttcaggaggc tgagtcgctc ctctcgcaaa tcgatgttaa ggatgcgact cgtccggagc
     1801 agatttcttt ggcagccctc gaggatatac gcaagaagat tgtgacggcg tatctcatgg
     1861 cgcagccaag tccctctcga gaaggtcggg cttattggaa ccatacggga ctgggggcct
     1921 atcccggcga ttgggaacga acggcccgtg agcttgccga agcaggcttt aacatggtcc
     1981 ttcccaacat gctttggggc ggggtggccc attatgcctc cgatttgctt ccccgaagtg
     2041 cgtcgttcga gaaatatggc gaccagattg cccagtgtgt cgaagcctgc catcggcacg
     2101 gtattgaggt tcatgtgtgg aaggttaact tcaacttgag tacggccccg aattccttcg
     2161 tcgaacagat gcgggcgagc ggtcgaacgc aagtcacccg ccgcggggaa cccactcgtt
     2221 ggctttgtcc ttcgcaccca gagaaccaaa agctggaatg cgaaacgatg cttgaggtgg
     2281 tgaagaaata cggcgtggat ggcattcatt tcgactacat ccgctatccc gacggcgaca
     2341 actgctactg cgatgggtgc cgtcagcggt tcgaaaaaga tacgggattg cgggtaacaa
     2401 gctggccaga cgattgctat agcggctcat tgagggcggc ctatcgaaat tggcggtgcg
     2461 agcaaattac gcgggttgtg cagatggtgg cgcaagaggc gaagcgactg cgggtggata
     2521 tcaaaatctc cgccgccgtt ttcgcggaat atccggcttg ccgggagtct gtcgggcaag
     2581 attgggtaag ttgggtaaaa gccggttatc tcgatttcat ctgccccatg gactacacgg
     2641 aggatgatcg gcgttttgag cgcctggtta agacccaggc ggaattgatc ggtggtcgca
     2701 tcccaatgta tccaggcatc ggggcctatc gcttggacgg cccggatcgt gttgtcggtc
     2761 agatttctat tgcccgtcag attggtgtgc ctggctttac gatcttcgat ctcaacgaac
     2821 agtcacccca gacgatcttc cccgcggtgc ggcttggtgc cactaaggag aaggccatcc
     2881 cggcacatcg tcatcaacct tagactggcg aaacgatcta tacttcgtta gtttttaggt
     2941 aaatcgacca gtgatcggcg tgttagcgcg atgtggtggt gtgtacccgg ccgctttggt
     3001 ttatgtaaag tgctgggcaa tttgcctgac ggctgcaatc gctgcttcga cgtgttcaag
     3061 tgtgttgaaa ggaccaaggc tgaaccgcac ggctccgccg cgttctaacg tacctagcct
     3121 acgatggatg aggggggcac aatggatgcc cgcacggact tggatatgga attgaccgtc
     3181 taaaatggcc gccagttcgt ggggactcag acctttaaca ttaatgctga ccacgccgac
     3241 acggtcggcc gcatttttgg ggccatagac ttctacccca gggatggcgt ggagtccgtc
     3301 aataaggcgt tgggtcaacg tgacctcatg tgcccgcaag tcgtccagac cacgctgttc
     3361 cagatactcg atccctttga tcaagcctgc caaagccggt gtattgggat ttcccggctc
     3421 atatctgtcg ggccattcgt ccggctggcg gtcttcgtcg ctccgcgaac ctgttccccc
     3481 gaaccgcagc ggaacgagtt cttcttccaa acccggtcgc acataaagaa tgcccgtccc
     3541 cccaggtccg agcagtccct tgtgccccgg cgcagcgaga aggtcgatac cgatctctcc
     3601 tgggaacagc ggcacatgcc caagggattg agccgcatcg accaaaacca tggcagagga
     3661 atgccgacga acggcgtcca cgatggcctc tagccgctgc agtgtgcccg tgacattgga
     3721 accgtgattc acggccacaa gacgggttgc gcgattgagc ttcgatcgca aggcgtccag
     3781 ttccacgagg ccctctggat cgcaggccac ataatcgacc cgtacaccgt aatgttccgc
     3841 caaccagcgg aggggacgaa gaaccgaatt gtgatcggct atggttgtga cgacgtggtc
     3901 ccccggtcga atcatgccgc gaagggccat gtttaaggca tccgttccgt taaaggcaaa
     3961 aagcacgtga tcggcctgcc cgacgcgcag ccatcgcgca acggccaggc gggcctccag
     4021 cacaacgcga tccgccgcaa gggcgtccgc ataggtgctc cgcccaggtg aacagccaat
     4081 ccgtcgttga tattcgtcca ctgcctgata cacagcctca ggcttgggcc aagttgttgc
     4141 cgcgttatcg aggtaaatgc gtgacgatgt catcttgaaa ctgtctataa atctgttgcg
     4201 agaccatgtc caattattgg agccggatca aggggagacc gcccaatggg tgccaagagc
     4261 gaggttactc tgccggttca aagaaggtga ggaacgattc aacggccgat tcgtgaacca
     4321 atgccacgac tgtatcgccc ggtttgatac gatcatcggc cccaggcaag atggcaaaat
     4381 cttcgcgaat caaggccgca atgaggcatt ggcggggcag acccacgcgg gcaaggacat
     4441 gttgcgtgat cagcgtgttg gggaggactt ccaattccat caccaggact ttggatcccg
     4501 gtgcaaacgg ttttttggca atcacgacgc cggtggtcaa gtgggcttcc acctcgctac
     4561 gcaccacatc cggtggcgtc gttacgtggt ccacgccaag cttggtgagg atgtaacgat
     4621 aatcggaacg ggccacaacg gcagccactt tctgggcacc caattccttg gcctcaacgc
     4681 aagccaaaat gttgttttca tcgtcaccgg tacaggcaac aaagaagtcg gcacttccca
     4741 cccgttcttc ttccagggct gcccggctgc gaacgtcgca gcacacaacg gtcacttcac
     4801 gcaaatgact agctaggaac tcgcaccgct cgtggtcgga ttccatcagg accacgttga
     4861 atcgcggtcc ttccaagagg cgtgccaggt gaaagccggt ttcacctccc cccgcaatga
     4921 ccacaccttg ccgccgcggt ggttccgtct ggaaaagcga tttcacggcg tcaatgtcct
     4981 cacgttttcc caccaaggca atgcgatcat tgacggcgac agcatcctcc gggccagcga
     5041 tgaaaacctt gtctccgcgg gaaatcacgc ccacgcggac atgccgaccc acacgaagct
     5101 cccgcaacgg gatgtcaatg gcaggtgcgc ccggctgaac cacaatttcc tgcatttcca
     5161 ggtcggctac ggcaaatgtc tcaatactga gggcgcccgg actacgaaca actcgggcga
     5221 ggtctaaagc ggcaaggcgc tccaagctaa ccaggtagtc caatttgaaa tgacgacgat
     5281 agtcgaacgt gcttgtgtcg agaagattgg gcgagtaaac gcgggcaatg gcccgacggg
     5341 tccccatcgc cttggccacg ctcgccccca cgagattgac ctcgtccgaa ttggtcaccg
     5401 ccaaacaaag atccgcgtcc gtcgcccccg cttgaaagag caaaacagca tcagccgcgg
     5461 acccgcatag ggcccgcacg tccatggtct cgttcacgcg atccacggct tcctgatcgc
     5521 ggtcaataag cgatacccgg tgggtctggc acaaaaactc ggcgatcgag gcgccaattg
     5581 tcccagcacc aagaatcaaa atcctcatgc gt
//
LOCUS       DAOSUT010000218         5600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6569_length_5600_cov_8.844184, whole genome shotgun sequence.
ACCESSION   DAOSUT010000218 DAOSUT010000000
VERSION     DAOSUT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_5600_cov_8.844184"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /gene="leuS"
                     /locus_tag="PLD05_14460"
     CDS             <1..2205
                     /gene="leuS"
                     /locus_tag="PLD05_14460"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOP78689.1"
                     /translation="IPLRQWMLRITAYADRLERDLDLLDWPESIKTLQRNWIGRSVGA
                     EVDFFIGTFWHKPGIPSQREYEGWRRDRELAGFPRKPDEACLRVFTTRPDTLFGATYM
                     VIAPEHPFVERLTLPEYREAVEAYREQAARKSDLDRTELSREKTGVFTGSYAINPVNG
                     QLIPIWIADYVLATYGTGAIMAVPAHDTRDFEFAKQFNLPIIPVVDPGDHASVSRQDV
                     LEGKVAFVEEGIAINSGPYNGLPTNEFKKRITEDLSEQGVGRQAINYRLRDWIFSRQH
                     FWGEPFPILHELDSSGKPTGRVRPLSPEELPLDLPSNMPFDAKHDSPEPPLEKAPPEW
                     LYVTIDGKKYKRETNTMPQWAGSCWYYLRFIDPKNDKALVDPELEKIWMPVDLYVGGA
                     EHAVLHLLYARFWHKVLYDRGLVSTPEPFQKLVNQGMILGEMEYTGYQDETGRWVSES
                     DVEEIGEARFVEKGTRIPLRPVKLRPDQVEKHGDVFVLKEKPEIRVDARAYKMSKSRG
                     NVVNPDDIVREYGADSLRLYEMFMGPLESTKPWSMEGVHGVRGFLDRVWRMIVDERAE
                     TLRLNAAVQDVPATEEQERVLHRTIKAVTDDIERMEFNTAIARMMEFVNFFFKQEIRP
                     KKVMETFVLLLSPFAPHISEELWQALGHQNTLAYEPWPTYDPELIKERVIEVPVQING
                     KLRGRVTVPPDIDREALAEAARAEPRIAALLADKEIVKTIVVPGRLVNFVVK"
     gene            complement(2231..3211)
                     /locus_tag="PLD05_14465"
     CDS             complement(2231..3211)
                     /locus_tag="PLD05_14465"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOP78690.1"
                     /translation="MSNCYLVTGCAGFIGSRVCEMLLADGHEVIGWDELNDAYDPRLK
                     QWRLERLQQHSRFWFSQIDVADQQAVERAFPKQIVAVVNLAARAGVRASVANPQVYYR
                     ANCLGTLVLLEMCRRYGVKKFLLASTSSLYGAHNPVPYREDTDTNRPLSPYAASKKAA
                     ETLAYTYHYLHGIDVSVVRYFTVYGPAGRPDMSIFRFIRQIAEGEPIVVFGDGTQRRD
                     FTYVDDIARGTIAALRPLGYEIINLGGDRSVELNMVIAEIARLLNKQPTVVHQPPHPA
                     DVPATWADVTKARTILGWTPQVPIEEGLRRCVEWYLEHREVMRTIRLGNG"
     gene            3526..5514
                     /locus_tag="PLD05_14470"
     CDS             3526..5514
                     /locus_tag="PLD05_14470"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOP78691.1"
                     /translation="MSEKGVISAEGQTRALSAAVWERTLREVLAAACEASDAISYLRF
                     QTPHLRRVLEAQFFGFLVFAGNGRSWEWVTTIGETKYATECDWGRLADRHGPLTEGPW
                     CLQPLAEGPIGRIWIAARFDHPQTAAQVRELLGELAETLNTALHFVSERSRISEQLKR
                     QTTLLRIAQRWYQARQLRPLLSEIAQAAAEFLRADRATIFLWNRDTKMLIGRPALGVD
                     DELRIPDDAGIAGRVLRTGQPVRVSSTSPDRDEIDRRVDKQLGYRTETLICVPLRTRG
                     GRVLGVFEVLNKKDGNFTADDETTLLELAEQAATALESVRDYEEALLTRRQIVDEAAR
                     RVQLIGQSPAIVRLRRDIERLAQADLPVLILGENGTGKEVVAQMLHYLGPRRESPFVA
                     VNCAAIPESLAESELFGHEKGAFTDAHQTRPGKFELAQNGTVFLDEVAELSLAAQAKL
                     LRVLEERKITRVGGTEAIPVYARIITATNQNLQQRVAEGRFREDLFYRLNTVILTLPP
                     LRERGDDIILLAEHFLAEFATRIRRPVPELTPEAKAFLMQYPWPGNVRELRNVMERVV
                     YLCSKTSIEPSDLGLHPEAAGVKRIGAFGPGLKLREATRRFQTEYILRTIEVSQNNVT
                     LAARQLGLHRANLYRKMRQLGIQLRRENEDSEPQPEKT"
BASE COUNT         1288 a         1521 c         1606 g         1185 t
ORIGIN      
        1 attcccctgc gccaatggat gctccgcatc accgcgtatg cggatcgcct ggaacgagat
       61 ttggatttgc tcgactggcc ggaaagcatc aagacgcttc agagaaactg gattggtcgc
      121 agcgtcggag cggaagtgga ctttttcatt gggacgtttt ggcacaaacc cggcattccc
      181 tcgcaacgag aatatgaggg atggcggcgc gaccgcgaat tggccggatt tccgcgcaag
      241 ccggacgaag cctgtctgcg tgtattcacg acgcggccgg atacgctttt cggcgccacg
      301 tacatggtca ttgcaccgga acacccgttt gtggaacgtt tgaccttacc tgaataccgt
      361 gaggccgttg aggcttaccg cgaacaggcg gctcgaaaga gtgatttgga tcgaacggaa
      421 ctttcccggg aaaaaaccgg tgtgtttacg ggctcgtacg caatcaaccc ggttaacggg
      481 cagttgatcc cgatttggat tgccgactac gttctggcaa cttacgggac cggggcaatt
      541 atggcggttc cggcccacga cacccgcgac tttgagtttg ccaagcaatt caatctgccg
      601 attatccccg tggtggatcc cggtgaccac gcctcggtgt cgcggcagga cgtgctcgag
      661 gggaaagtag cctttgttga ggaagggatt gccatcaatt caggcccata taacggcctg
      721 ccaactaatg agtttaagaa acggattacc gaggacctct ctgaacaggg agtaggtcgg
      781 caggcgatca actatcggtt gcgggattgg attttcagtc gccagcactt ctggggggaa
      841 cccttcccca ttcttcacga attggacagt tccggaaagc cgaccgggcg ggtccgtccg
      901 ctctcaccgg aagagttacc tctcgatctc cccagcaata tgccgttcga cgcgaaacat
      961 gactcgccgg agcctccgct ggaaaaggca ccaccggaat ggttgtacgt gaccatcgac
     1021 ggcaaaaagt acaagcgcga aaccaacacc atgccgcagt gggcaggttc ctgctggtac
     1081 tacctgcggt ttatcgaccc gaagaacgac aaggccttag ttgatcccga acttgaaaag
     1141 atttggatgc ccgtcgattt gtacgtcggt ggggcggagc atgcagtact gcatctgctt
     1201 tatgcccgat tttggcacaa ggtcctttac gatcggggcc ttgtcagtac ccccgagccg
     1261 tttcagaagc tcgttaacca ggggatgatc ctcggcgaaa tggaatacac gggctatcag
     1321 gacgaaaccg gccgatgggt cagcgaaagc gacgtggagg aaatcggcga ggcccgtttt
     1381 gtggaaaaag gaacgcggat acctctccga ccggttaaac ttcgccccga tcaggtagaa
     1441 aaacacggag atgtatttgt tcttaaggag aaaccggaaa ttcgtgtcga tgcccgggca
     1501 tacaaaatgt caaaaagtcg gggcaatgtt gttaacccgg acgatattgt ccgcgaatat
     1561 ggggcggact cgttgcggct atacgagatg tttatggggc cacttgaatc gacgaaaccg
     1621 tggagcatgg agggtgttca cggtgtccgg ggattcttag accgggtgtg gcgaatgatt
     1681 gtggacgaac gtgcggagac cctccgtctc aatgcggcgg tgcaggacgt ccccgcgacc
     1741 gaggaacagg aacgcgttct ccatcgcacg atcaaggcag tgacggacga tatcgagcgg
     1801 atggagttca acaccgccat tgcccgaatg atggaatttg tcaatttctt cttcaagcaa
     1861 gagatccgtc ccaagaaggt catggagaca tttgtgctcc ttctctcccc attcgcaccc
     1921 catatcagtg aagagttgtg gcaggccttg ggccatcaaa acacgctagc ttacgaaccc
     1981 tggcctactt acgaccctga gttgatcaag gaacgcgtca ttgaggttcc tgtgcaaatc
     2041 aacgggaagt tgcggggacg ggtgacggta cctcccgaca ttgatcgaga ggccctggcc
     2101 gaagccgcgc gagctgaacc gcgaatcgct gccctgctcg cagacaaaga gattgtcaag
     2161 accatcgtcg tgccgggacg actggtcaat ttcgtggtca agtagcgctc aggcaagatc
     2221 gtaaaggaaa ttatccgttg cccaaacgga tggtgcgcat cacttcgcgg tgctccagat
     2281 accactccac gcaccgccgc agtccctctt caatcgggac ttgcggggtc caacctagga
     2341 tcgtccgggc cttggtaaca tcggcccacg tggcgggaac atcggccgga tggggtggct
     2401 gatggaccac cgtgggttgc ttgttgagca gtcgtgcgat ttcggcaatc accatattca
     2461 actccacgga acgatctccc ccgaggttga tgatttcgta acccaggggg cgaagggcgg
     2521 ctatggtacc tcgagcgata tcatccacat acgtaaaatc gcgccgttgg gtcccatccc
     2581 cgaagacaac gattggttca ccctcggcga tctggcgaat aaagcggaag atgctcatat
     2641 ccggccgccc agcaggcccg taaaccgtga agtaccggac aacactcacg tcaatcccat
     2701 gaagatagtg ataagtgtaa gccaaagtct cagccgcctt tttggatgcg gcatatgggg
     2761 acagcgggcg attggtgtca gtgtcctctc gataagggac aggattatgc gccccataaa
     2821 ggcttgaggt ggaagccagg agaaatttct tgaccccata ccggcggcac atttctaaca
     2881 ggacgagtgt ccccaggcaa ttggctcggt aataaacctg ggggttagct acgcttgctc
     2941 ttacgcccgc ccgagctgcc aggttgacca cggcaacaat ttgcttcgga aaagcgcgtt
     3001 caaccgcctg ctgatcggcc acatcaattt gcgagaacca aaaccgcgaa tgttgctgca
     3061 acctctccag tcgccattgc ttgagccgcg ggtcgtaagc atcattcagt tcatcccacc
     3121 caatgacctc gtgaccatca gccaaaagca tctcgcagac ccgcgatcca ataaagcctg
     3181 cacagcctgt caccagatag caatttgaca tgattcttcc tcaccccgac gttgcacgct
     3241 accgacctgc gtcaccttgg ctatggtttt cgaaagactc cattctaaac gagaaaccca
     3301 atcagagtaa tggcacaata agggcaaagt gtggctatca ttggtcccgc aaaagagtat
     3361 ttttgtaacg gggcgctgac tgttttcttc aaaccctcca ccttctccca gtgcgctctg
     3421 gtatgatcaa agcaaggaac gcgataaaat aggcttgcta ctgtcggacg agcatcttgt
     3481 ccttgagctt tttgaaagac attgatcata tcgaggagta cggttgtgag tgaaaaagga
     3541 gtcatttctg ccgagggcca gacacgcgcg ctatctgccg cggtatggga acgaacactg
     3601 cgggaagtcc ttgcggctgc ctgtgaggcg tccgacgcca tcagctattt gcggtttcag
     3661 accccgcatt tgcgacgcgt cctcgaagcc cagttctttg ggtttctcgt cttcgcaggc
     3721 aacggacgtt cctgggaatg ggtaaccacc atcggcgaga caaaatatgc aacggagtgt
     3781 gattggggga ggctcgccga tcgccacggc ccgctgacgg aaggcccctg gtgtctgcaa
     3841 ccgttggcag aggggcctat cggccgcatt tggattgcgg cccgttttga tcatccccag
     3901 acggcggcac aggtccgaga actccttggg gagttggcgg agaccctcaa tacggccctt
     3961 cactttgtga gtgaacgatc ccggatttca gaacaactca agcgtcaaac cacccttttg
     4021 agaattgcac agcggtggta tcaagcgcgg cagcttcggc cccttttgag cgagattgcc
     4081 caggccgcgg ccgagttctt gcgggcagat cgggcgacga tcttcctttg gaaccgggat
     4141 accaagatgc tgattggccg ccctgcccta ggcgtagatg atgagctccg aattccggat
     4201 gatgccggca tcgccgggag ggtcttacga accggtcagc cggtccgtgt ctcaagcacc
     4261 tcgcccgacc gtgatgagat cgaccgccga gtggacaagc agctaggcta ccgcaccgag
     4321 acacttattt gtgttccgct gcgtacccgc ggggggcgtg ttcttggggt cttcgaagtc
     4381 ctcaataaga aagacgggaa tttcacggca gacgacgaga ctaccttgct tgaacttgcc
     4441 gaacaagcgg ccacggcctt ggagagcgtg cgagattatg aagaagcctt attgacaagg
     4501 cgacaaatcg tagatgaagc ggcacggcgg gtgcaactga ttggacaatc gcctgcgatt
     4561 gtccgtctcc gccgggatat cgagcgattg gctcaggccg atttgcccgt actgatcttg
     4621 ggcgaaaacg gtacaggcaa ggaggtcgtg gcccagatgc tccattacct tggcccacgt
     4681 cgtgaaagtc ccttcgtggc cgtcaattgc gcagctatcc ccgaatcgct agcggaaagt
     4741 gaattgtttg gccacgaaaa gggtgccttc acggatgccc atcaaacgcg accgggcaag
     4801 tttgaattgg cccaaaacgg gacggtcttt ctcgatgagg ttgccgagtt aagtctggcg
     4861 gcccaggcca aactcctgcg ggtcctcgag gaacgaaaaa tcactcgtgt tgggggaacc
     4921 gaggccattc cggtttatgc ccgaatcatt acggctacga atcaaaacct tcaacagcgg
     4981 gttgcggaag ggcgctttcg cgaggatctt ttctatcgcc tgaatacggt aatcctaact
     5041 ttgccgccac ttcgggaacg aggggatgac attatcctct tggccgagca tttcctggcc
     5101 gagtttgcca caaggattcg acgtcctgtg ccggaactca cacccgaggc caaggcattt
     5161 ctcatgcagt acccctggcc gggcaatgtg cgagaactgc ggaatgtcat ggagcgcgtc
     5221 gtttacttgt gctcgaagac gtcgattgaa ccgtccgatc ttggcttgca cccggaggca
     5281 gcgggcgtga aacggattgg ggcctttggc ccgggactta agctccgtga agcgactcgg
     5341 cgattccaaa ccgagtatat cctgcgaacg atcgaagtct cgcaaaacaa cgtaaccttg
     5401 gcagcccgac aattgggtct gcaccgagct aatttgtacc gcaaaatgcg gcaattgggc
     5461 atccagttgc ggcgggagaa tgaggactcc gaaccacaac ccgaaaagac gtgattgaat
     5521 ggcaacccgt atcggcgcag ggcgggcatg gccacgaagg cgactaagcg ggtcttactc
     5581 aagatggatt cccacttgct
//
LOCUS       DAOSUT010000219         5550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6630_length_5550_cov_9.171975, whole genome shotgun sequence.
ACCESSION   DAOSUT010000219 DAOSUT010000000
VERSION     DAOSUT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_5550_cov_9.171975"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLD05_14475"
     CDS             <1..356
                     /locus_tag="PLD05_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78692.1"
                     /translation="RHGGLPLRFDWVGATPRGCAVLGAVRSLRVRHGARGSSGTGPTG
                     VPALICNAVRRLFRYGRPVRLLPAPAWMRNESSRRCQKRIWARIFTSAGGMCRGLSRA
                     VNHLRNAARQAIVAN"
     gene            764..1105
                     /locus_tag="PLD05_14480"
     CDS             764..1105
                     /locus_tag="PLD05_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78693.1"
                     /translation="MSPYEALLRSHHLDVDKPGMCVEKDGYGGIMVYQTYPGTDLPDF
                     SKPAICVEKDGYDRMMVYQTYPGTNIPDSRKPGIYVERDDDGRMMLYETYPGTNIPNL
                     RKPAIRIESDY"
     gene            complement(2722..3012)
                     /locus_tag="PLD05_14485"
     CDS             complement(2722..3012)
                     /locus_tag="PLD05_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78694.1"
                     /translation="MLQNYKSGNNSDGKLTTHQSVDQIVRSIYDIVRWDICAEARQNV
                     PTLTRLLFSRILDAREQHETQNPEAVGVACSPLGGTLSLARLGCTIGRGNNH"
     gene            complement(3917..>5550)
                     /locus_tag="PLD05_14490"
     CDS             complement(3917..>5550)
                     /locus_tag="PLD05_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOP78695.1"
                     /translation="STRRDGKLELSDIASYWGHGTNSATPAIARIDCSFVPESSSGKS
                     ESSRVDPQWVIDRSLDDRSATWFSHGRNRSERGFLTLYFEKKYRPVEVRLWAERLPRQ
                     FTVYVVSAADVPKTAESLTDWEKKHENNSVAQYPNFSNKQFESQQESPFDVRNPVRIR
                     LSRQREGEAVVLVWQEAINITLREIEVVVQPTYCGDLAFGAAVYDAETGLYYHGARYR
                     LPELGHFIAPDPLGFLGGDNLYAFAHNDPLSWHDPDGRFAHVLLGAGTGALFGAGSYL
                     AQWLRYGEEFSWKRLAVYTASGAASGAVGALVAPFALSVGGYYSLSATWNAILSSSLA
                     GASAGAAGGAIQSGGLTYLRTGDIGESLKAAGFGALGGAAFGAIGGVIGGSVVSRTGG
                     SFRGFVLSGAAGGGVVGFAVDAYKGYRETGTLVGTVKYGLWGAGKGAAVGAAIGGAAW
                     GIGRVTGWIRRLPEQPEGLPDPREEGILIRTKPRRGDYGNVEVEPGYARHHKKPLSLG
                     GTDDPSNIVKVPVEIHRQPHPGREVIEAPLGTIFY"
BASE COUNT         1236 a         1563 c         1506 g         1245 t
ORIGIN      
        1 gcaggcacgg gggcctgccc ctacgttttg attgggtagg ggcaaccccc cgtggttgcg
       61 ctgttcttgg cgctgtccgc agtctccgtg ttcgacatgg tgcccggggt tcttctggta
      121 cggggccaac cggtgtgccc gccctaattt gcaacgctgt ccggcgattg ttccgatatg
      181 ggcgacctgt gcgattattg ccggcacccg cttggatgcg gaacgagtca agccgccgct
      241 gtcagaaacg gatctgggcg aggattttta cctccgcggg aggaatgtgt cgcgggctgt
      301 ctcgggcagt caaccacctc cgcaatgcag cgcggcaagc catcgtggcg aattgaagcc
      361 actcacaaac cttcattagg gtggacgact tccctgtgag gtgcaacttg cagccgtcgt
      421 agctgcaccc agaaagccga gcgcttgaca gggagtatgc tcctatttac gataatctgc
      481 gcttggtgcc gtgcccttgc agcgtcacga caggcatgac gatccggtgg ccaatagcat
      541 tacgggagac tctgttatgt cgctattcga ccggcttgca gatggatccg gtttgagtcc
      601 tctcggtgtt gctgtagtgg tgctcgcaat gagtcttttg caaggtggcg acaaatcgga
      661 cgggcctgct cctccccgca aaagccccgc tacccctgtg agcattggag tgcgtgggta
      721 catgggtact tcgattccgc gtcagccata taggtgggag aggatgagcc catatgaggc
      781 ccttctgagg tcgcatcacc tcgatgttga caaacctggg atgtgcgtcg aaaaagatgg
      841 ttacggaggt atcatggtgt atcagaccta tccgggcacc gatcttccag atttcagcaa
      901 accggcaatt tgcgtcgaaa aagatggcta tgaccgtatg atggtgtatc aaacctatcc
      961 gggaacgaac atcccggatt ctaggaaacc ggggatatat gtcgaaaggg atgacgatgg
     1021 acgtatgatg ttgtatgaga cctatccggg gacgaatatt ccgaatttga gaaagccagc
     1081 gatacgcata gaatcggact attaaggcat ttgttggccc aggggtcgcc acccgcccgg
     1141 aatgctttgc tgatgcagga ttactctgac tttgccgcct aacgcaaaat ccgctgtccg
     1201 taggcgatca cgaatctcca ttgcgaacgt tgccgaagcg gaagccaagt cgtcaagagt
     1261 gagtttcggg gtctcgtcaa cgggtttggg gcttatccca aaatgacgtt tctcgaaaga
     1321 aaactctgag tcacagtatc ggatgctgtg gcaacccggt tagcgaagga ggtctggtag
     1381 accacatgga acctctgctt ccgtaatgga agcgaggggg ccgttatccg gggcttaagt
     1441 gatttggagt ggcggcaagg ggtcaagtaa attttcaatg agctctttac aggctgccag
     1501 tgaaaagtgg agccggggag ttcggttccg gcttcttgag aagcttgtgg ggtgtggggc
     1561 gatcgagctt ctctagaaga taccactcgg ggaacccgac gagttacaaa gtattcaccg
     1621 ggaatggcgg gccctcgacg ggttgttgag caagttccct ctgcgaggtc aaaagctggg
     1681 cctattccta tggattgaac caatcgagcg cacgtttttg gttcaatcct ttccggaggc
     1741 tgctgatttc gttgggaacg ctgggaaaag aaggcggaaa actacgctgc ccttggccct
     1801 ttgctatcac gcctgcgcgc gggtattcat ctgcacgtgg ctcccaatcc gcgctgccga
     1861 ctcttcgaga taggctgtta gttcagtgga ggcactaccc ttttcatgca tgccgagaaa
     1921 caaccgaatg ccgcttcgcc aaaacggcgt ccaagccggc gcgtgtttgc caaagcaaca
     1981 ccggttgtgg tgaggcgtgc cgtttcaccg attcctttct cggtcggcaa cagattcctc
     2041 cgcgatcgcc acgatctggc gttgaacttc caagggcaac cgtggccaca gagcggctaa
     2101 ccgctcccac gttgcaagtg cagcttcatt gtcccatccg ctggggttac cgatggtgaa
     2161 tctctcatgg tcgttacatc catcgctgtg cggtgaatcg tcgtcaagtg tgccggattc
     2221 tgtgccaatt gacgggacag cctcgactta actcacggtt ttttggccac ttacgtcaac
     2281 aacgtcgatt cccgcgggat aaaaaaaaga aagtctgctc accattgcga ctggtcccgg
     2341 cgggagggtt gaaagttttc ggctagcaag gacccatgga agacggaggg ggtgggcgcc
     2401 gtctcctcca ccgccacccc accaaatcat accacgtctc gcggcgccca cccttggcca
     2461 ctcaaattac gcaagatata ggtcatgaga aagcccctac tttgtgggtt ccttaacggt
     2521 cggcgctcgt cggttcaaca tccccgttgt gcgtggacaa acccggttcg ttggtccagg
     2581 ggaagcctag ccatccgctc ccgcgcagaa agtgcagcac tatgtccagc agcttgacag
     2641 cgtcaactgc gcaagcatct tccaaataat aacttgcgtc atcaagtgcg aatcgcttcc
     2701 agccattctt cgccgttcac atcaatggtt atttccgcgt ccaatggtgc agcccagtcg
     2761 cgccagcgat agggtacctc cgagggggct gcaggcgacg cccacggcct cgggattttg
     2821 ggtctcgtgc tgctcccgcg cgtccaggat gcgtgagaaa agaagccggg tcagtgtagg
     2881 cacgttctga cgcgcctcgg cgcagatgtc ccaccgcacg atgtcgtaga tactcctaac
     2941 gatctggtca accgactggt gcgtggtcag tttgccatcg ctgttgttcc cgctcttata
     3001 gttctgcagc aaacacccga tgtaatatca gtccaggtat tgcccttatg gctttggctt
     3061 ccgctcggag aactatatcc aagggtcttc gggagaattt gagggtgaaa tcccagtccc
     3121 tcagtggtcg aaacgtgcgt tttttgatgc atagcggggc aaataaatga cgagaaaacg
     3181 cttttattgc cgggcttttt gggcggttta gcggccgaga tcgcgtggta cccggcccct
     3241 gcccgttctc ctgcagaccg agaaacaacc gcaggaattt gtgccccaaa ttgtcggctt
     3301 cgaagaacct cgaaaaatca gaaatcctcg gatttcagtg gtgtttacgc ccaaaaccca
     3361 cccacaaatt ctgagaaaga ggccttaatc ttttgtcaca atactgcgaa taaacgcaga
     3421 gaatgaagat gcaagctgct tcgtagcctc ggtttcgtgt agccagagat agagtgggct
     3481 cgtcggcttc ggcgccacaa gctccgcttt gagaaatccg aattcttcaa aagcatccct
     3541 tccaataagc agcaagggaa agtcagctgg tcgatctcgg ttggcagcga gaatcgtctc
     3601 cgctgggtgc aagccaggac caacggagta aaggaggaac cttttgccag gaagtatgcc
     3661 tccgtcagcc cacaaaagga attcgacata ctcctgaggg aaatgtatgc ccagtgagcg
     3721 agcaacctct tcaattacac caggatccgc aggtggcaat agttcgcctc ggtcacgcaa
     3781 caattgttcc ggccaagtca tgattaacgt tccttcctct gcgagatgtg aatacgtctt
     3841 ttaggctggt cgatccaagt tacgacctag attgcctgct gctccagttt cacggggcaa
     3901 ccgctccaag gtccgatcag tagaagatag tgcctagggg agcctctata acttcgcgcc
     3961 ccggatgagg ttgtcgatga atctcaaccg gcactttaac gatattactc gggtcgtcgg
     4021 taccccccaa tgacaagggt ttcttgtggt gccgcgcata gccaggctca acctcaacat
     4081 ttccatagtc accacgacgt ggcttcgtgc gaatgagaat accctcctct ctcggatcag
     4141 gaagtccctc tggttgttca ggaaggcgac gaatccagcc agtgacccgg cctatacccc
     4201 aggctgcgcc acctatcgct gccccgacag cggcgccttt gccagctccc cataggccat
     4261 actttactgt cccaactagt gtccctgtct cgcgatatcc tttgtaagca tctacggcga
     4321 aaccaaccac cccccctcca gcggcaccac tcagaacgaa tcctcgaaaa gaaccgccag
     4381 tccgcgaaac aacgctacct ccgatgacgc caccgatcgc gccgaaggca gcgcctccga
     4441 gtgctccaaa acccgccgct ttcaggctct ccccgatatc gccggtcctc aaataggtaa
     4501 gtcctccact ctgaattgcc cctcccgctg cccctgccga tgctcctgcc aaagaagagg
     4561 acaaaatagc attccatgta gcagataagc tgtagtaccc tcctacagat agggcgaatg
     4621 gcgcgaccaa tgcgccaaca gccccgctag ctgctcccga tgcggtgtag actgccagcc
     4681 gtttccaact gaactcctcg ccgtaccgca accactgcgc aagataactg ccagcgccga
     4741 atagcgctcc ggtgcctgcc cctaagagaa catgagcaaa gcgtccgtcc ggatcgtgcc
     4801 aagatagggg atcgttatga gcgaacgcgt agagattatc ccctcccaaa aagcctaggg
     4861 gatcaggcgc gataaagtgt cccaattccg ggaggcggta ccgcgcgccg tgatagtaga
     4921 ggccggtctc cgcatcatag acggctgccc cgaatgccag atcaccacaa taggtgggct
     4981 ggaccaccac ttcgatttcc cgaagtgtaa tgtttatcgc ctcttgccat accaaaacaa
     5041 ccgcctcgcc ctcgcgctgc ctggaaaggc gaatacgcac cggatttctg acatcgaagg
     5101 gggactcttg ttggctttca aactgcttgt tagaaaagtt cggatattgg gccacggaat
     5161 tgttttcgtg tttcttctcc cagtcggtaa gagattccgc tgttttgggc acgtcggcag
     5221 ccgaaaccac ataaacagtg aattgccgag ggagccgctc tgcccagagg cgtacttcta
     5281 ccggccgata cttcttttca aaataaagag tgaggaaccc cctttcactt ctgttcctgc
     5341 catgactaaa ccatgttgcg gaacgatcat ccaaagatcg gtcgatgacc cattgcggat
     5401 ctacgcgcga actctccgac ttgcccgacg agctctcggg tacaaaagag caatcgatgc
     5461 gcgcgattgc cggcgtggcg ctgttagtgc catggcccca atagctggct atatctgaaa
     5521 gttccaattt gccatcgcgg cgtgtgctgc
//
LOCUS       DAOSUT010000220         5531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6656_length_5531_cov_10.100438, whole genome shotgun sequence.
ACCESSION   DAOSUT010000220 DAOSUT010000000
VERSION     DAOSUT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6656_length_5531_cov_10.100438"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLD05_14495"
     CDS             complement(<1..341)
                     /locus_tag="PLD05_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655651.1"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOP78696.1"
                     /translation="MSDPYFQKLFAERIGGTQYGKGTEIYKFEKIKRAKRRVLAEHPE
                     RPLIDFGIGENDEMADESVRAVMAEEINKLENRGYADNGIPAFKEAVARFMEREFGVK
                     LDPTSEINHCIG"
     gene            936..1952
                     /locus_tag="PLD05_14500"
     CDS             936..1952
                     /locus_tag="PLD05_14500"
                     /inference="COORDINATES: protein motif:HMM:NF024906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78697.1"
                     /translation="MSLSAPRSLIAIRYWVIVICISILATSGCQRETTNGPRSESTPQ
                     ATGSAQGERPTGQPPREGDSALAGKLEALGVRVRLDDDGYIISVDASSSGIDDAQLAI
                     LKDAHRIRSLKLTGCPITDAGLDTIATLINLKSLGLDQTQVTDAGLAKLQSLPHLEEL
                     FLAGTAISDEAVKAISACKKLRRLRLRGTKITDAGLSYLKALPELTEIDLSETAITDQ
                     GLMVLSEMPSLSRLNLYTTQVTDAGMAAFEKMPQLVWLNLDNTKITDAGLPHLKGLTR
                     LEFLHLGRTAISDNGIPRLFDLKALKTLHVTRTNVTEAGGEKLRQALPNCEVITQVQE
                     SPQT"
     gene            2018..3007
                     /locus_tag="PLD05_14505"
     CDS             2018..3007
                     /locus_tag="PLD05_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOP78698.1"
                     /translation="MLPEKKWVLELTDGFRVAAIVGAAAELGLFEVIPEAGITAEGLA
                     SQLSCSLRGVRILCDALTALSILEKQGDRYYLPAKLRPVLRETEPDTAIPMLRHRMAM
                     MRGWAQLAWTVKAGFPAPRTSSIRGPDGDLASFVLAMNVVSRDVADDIVRQLQPLQFR
                     RMLDVGAGPGTWAFAFLRAVPEATAVLFDLPHAIALARRKAEEAGLADRIEFVVGDFY
                     VDELPQDVDFAWVSAITHQNSREQNRQLFQKVFRALRPGGQIAIRDIVLEPDRTAPLE
                     GALFAVNMLVSTPSGNTYTLDEFREDLLAAGFSEPHLRIRAENMNSIILAHRR"
     gene            complement(3036..3746)
                     /locus_tag="PLD05_14510"
     CDS             complement(3036..3746)
                     /locus_tag="PLD05_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOP78699.1"
                     /translation="MIDRENASLDLREAEAPEVVPREGLGRRVGKVFQILAMLCVLGL
                     FALVTIKYSPFNPDRVPVGVNHPSVGKQLEVISLTPLYGEGGILDRSGVSGKVVLLNF
                     WGTWCPPCRAEFPELVSLVRKFAHDSRFQFLSVSCAPPGADGDLEELRQSTQAFLLEQ
                     NASDVSIYADPDYLTRREVDRVAGFSVYPTTILLDEKGVIRAVWQGYWRGLDREIEES
                     LRHVLAQYQASGEPPSSK"
     gene            complement(3793..4284)
                     /locus_tag="PLD05_14515"
     CDS             complement(3793..4284)
                     /locus_tag="PLD05_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78700.1"
                     /translation="MSIADTATPKTQYWQPLSKADPQPDNPVAAAIWQVISRIYPNAP
                     QTERRQAPRYAFPYLVQIQPVFEDGVSRYGSPFVVVGKQISELGLGFYHPKPLPSRYG
                     IVSLDAGGTQISLLIELLWTQFTHHGWYESGGRFVRLLGSPHSNGNMTGVHAAQTADR
                     MNF"
     gene            complement(4633..>5531)
                     /locus_tag="PLD05_14520"
     CDS             complement(4633..>5531)
                     /locus_tag="PLD05_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78701.1"
                     /translation="FLLRKFADQMKRIQARVDPRMPPLVRLQQAFRALHSEILYGGYF
                     IGASNPGEAIQSGRFNCVSAAILFKLVADSLGFEVRVLQSPTHAYCLVRCEDNWVPVE
                     CTCPTWFDNLSNRDQLAQDVDRQNFGKTLSNGNRLEHPMVSRAQQSEGTPDPKSGRSI
                     SETQLLGTIYYNRGVASLLDRQYEVAVVSNLRAIQLDPENQSAQDNLLASLNNWAIAL
                     AERREFAEAATRLMLALECRPDSGPIQANLYRVCREWKNELSVQGKDEEVKAQLGRVI
                     ESLSRHPCCTELRTKIGSLLTY"
BASE COUNT         1319 a         1542 c         1440 g         1230 t
ORIGIN      
        1 ccgatgcaat gattgatttc ggacgttgga tccaacttaa ccccaaactc gcgttccatg
       61 aatcgcgcaa cggcttcctt gaaggcggga atgccgttgt cggcatatcc tcggttttca
      121 agtttgttga tctcctcggc catgacggca cgaacgcttt cgtcggccat ctcatcgttt
      181 tctccgatgc cgaagtcgat caacggcctt tcggggtgct cggccaagac acgccgtttt
      241 gccctcttga tcttctcgaa cttgtagatc tctgtacctt ttccgtactg ggtgcctcca
      301 atccgctcag caaagagttt ttggaaatag ggatcgctca tggaactaga cacctgactg
      361 ctcaacaaac cacaggagct ctgtctaaag aaacttcttt tcgaccattt ttctctcgca
      421 aaaatttatt gtcgcgtttc cgcacatctc tgcaaggaat agcacttgtg cccgatttgg
      481 gacggatatc cgaagttaaa tcggagtgta tcccgagatc acgcaggctt gagaaacggc
      541 acacgtgctg aatcagttcg cccttgagca atcacaatcg gccatgtagc ggacaagcag
      601 gtgctttgcc gacaacctcc gaggtgttta agagcctatt ccaaaaagcc gccctctccc
      661 tccgggagag ggttggggtg agggcaagcg aacgtcggaa tcaattgaga aaaccggcag
      721 ccatccgagc caccctcact cccgacccgt ctcccgccga acgcgcaagg agagattatg
      781 gcacaggctt taatgtcctt ttttcaggta cggattgcgt cgagatttca aactctgtgt
      841 aatgatacct tatgtgattt cgcggatgct ggtccggttc gcgaagtaat actggccaaa
      901 taaccttcaa aagcccgtta acgaaatcaa aagtcatgag tttatctgcc ccacgatctt
      961 tgatagccat tcggtattgg gtcatcgtca tttgcatttc cattctcgcc acaagcggat
     1021 gccagcgtga aaccacaaat ggcccacgct cggagagtac accgcaagcc accggctctg
     1081 ctcaagggga acggcctacc ggtcaaccac cgcgtgaggg tgactcagcc cttgccggca
     1141 agcttgaagc cctcggggta cgtgtccgcc tggatgatga tggctacatc atttcggtag
     1201 atgcgagctc cagtgggatt gacgacgccc aacttgccat cctcaaagat gctcatcgca
     1261 tccgatcgtt gaagctaacc ggttgcccaa tcactgacgc aggattagac acaattgcga
     1321 cgttgatcaa tctcaagagt ctcggcctgg accaaaccca agtaaccgac gcgggccttg
     1381 ccaagttaca gtcactacct catttggaag agttattcct ggccgggacc gcaatatcgg
     1441 atgaggccgt aaaagcgatc tcggcttgca agaagttgcg acggctccgt ttgcggggaa
     1501 ccaagattac ggacgcaggc ttgagctacc tcaaggcact cccggagtta accgaaatcg
     1561 atttgagcga gacagccatc acagatcaag gcctgatggt gctgagtgaa atgcccagtc
     1621 tttcgcggct taatctttac accacgcagg tcacggatgc cgggatggcg gccttcgaga
     1681 aaatgccgca actcgtgtgg ctcaacctag ataataccaa gatcaccgat gcagggcttc
     1741 cccacctgaa gggtttgacc cgccttgagt ttctccacct cggtcgaacg gccatcagtg
     1801 ataacggaat cccccgtctc ttcgatctca aagccctcaa gacgctccat gtcacaagaa
     1861 cgaacgtcac ggaggcgggc ggcgagaaac ttcgccaggc ccttcccaac tgcgaagtca
     1921 taacccaggt gcaagagtcg ccacaaacct agtgaagcgc cgtcacctaa agagacgtgt
     1981 tcaacagtcc ggcacataag ggtatcaagg agtaaacatg ctaccggaaa agaaatgggt
     2041 tctggaattg acggatggtt ttcgagtggc agcgattgtc ggtgctgccg ctgagcttgg
     2101 cctattcgaa gtcatcccgg aagcgggaat cacagctgaa ggcttggcca gccagctctc
     2161 gtgttcgctc cgcggtgtca ggatcttgtg cgacgcccta actgcgcttt ccattttgga
     2221 aaagcaaggc gatcgatact acctaccggc aaagctccgc cctgtgttgc gtgaaaccga
     2281 accagacaca gccattccga tgctgcggca tcgtatggcc atgatgcggg gatgggcaca
     2341 actggcctgg acggtcaagg caggttttcc ggcccctcgc acttcgagta ttcgcggacc
     2401 ggatggggat ttggcatctt tcgtcttggc aatgaatgtc gtttctcgcg atgtcgccga
     2461 tgatattgtg cgccaactcc agccgctcca atttcggcgg atgttagacg taggcgccgg
     2521 gcccggaacc tgggcgttcg cgtttctccg cgcagtcccg gaggctacgg ctgtcctctt
     2581 tgatctgcct cacgccatcg cgctggcaag gcggaaagcc gaggaagcag gacttgccga
     2641 tcggatcgaa tttgtcgtgg gcgatttcta cgtcgatgaa ttgccacaag atgtggactt
     2701 cgcttgggtc agcgcgatta cccaccaaaa ctctcgcgag cagaaccggc agctcttcca
     2761 aaaggtcttt cgggccctca gacccggagg tcaaatcgcc attcgtgaca ttgttctcga
     2821 acctgaccgt acggcccctc ttgagggcgc cctgttcgcg gtcaatatgc tggtgtcaac
     2881 tccatctgga aacacctata ctttggacga gtttcgcgag gacttgttgg ccgccggttt
     2941 ttcggaaccg catctgcgga ttcgagcgga aaacatgaat tcaatcattc tcgcgcatcg
     3001 gcgctaagtg atgtctggtg cggctggtca gtgagttact ttgacgaagg tggctcgcct
     3061 gaagcttggt actgtgccaa tacgtggcgc agcgactctt caatctccct atccagtcct
     3121 ctccagtaac cctgccacac agcccgaata acacccttct catcgaggag gattgtggtg
     3181 ggataaaccg aaaagccggc cacccgatcg acctctcgac gcgtaaggta gtcaggatcg
     3241 gcgtagatgg agacatccga ggcattctgt tccaacagaa aagcctgcgt agattggcgt
     3301 aattcctcta gatcaccgtc tgcaccgggt ggggcacacg agaccgagag aaattggaat
     3361 cgcgaatcgt gagcgaattt ccggacaagc gacaccaatt caggaaactc cgcccgacag
     3421 ggcgggcacc aggtccccca aaaattaagg agcacaactt tgcccgatac tccggagcga
     3481 tcgaggatac caccttctcc atagagcggc gttagggata ttacttcgag ttgtttaccg
     3541 acacttggat ggttaacgcc aacagggacc cggtccgggt taaacgggga atacttgatc
     3601 gttaccaggg cgaaaagccc cagcacacaa agcatggcga ggatttgaaa cactttacca
     3661 acccgacgcc ccaaaccctc tcgtgggacc acctcagggg cctctgcttc tcgcaaatct
     3721 agactagcat tttcacgatc aatcatacgc ggcggcacaa cacgcaccaa cggacatgca
     3781 aatgagagtc gttcagaaat tcattctatc cgcggtctgc gccgcatgaa cacccgtcat
     3841 gtttccatta gagtgagggg aacccagcaa ccggacaaat cggccaccac tttcatacca
     3901 tccgtggtga gtgaactgag tccaaagtaa ctcgatcaac agggaaatct gtgtgcctcc
     3961 ggcgtcgagt gaaacaattc cataacgact tggcagcggc tttggatgat aaaaccccaa
     4021 ccccagctct gaaatctgtt tgccaacgac aacaaagggt gaaccgtaac gggagacccc
     4081 atcctcgaaa acaggctgaa tttgaacaag atacggaaac gcgtaacgtg gtgcctgccg
     4141 ccgctccgtt tgaggcgcat tcggataaat ccgcgaaatg acctgccaaa ttgctgctgc
     4201 cacaggattg tcgggctgcg ggtcagcttt cgataagggt tgccagtatt gcgtcttggg
     4261 cgtagccgta tccgctatgc tcatgctcgt ccctccctta cagcgatcct atgggcggcg
     4321 gcagaaagac atcctaaaac aggtttcgcc gatgatgccg caccctgaaa ctcgttggtt
     4381 attgcgctga cgcgacgggc acagctcgga gtcattccaa accagttttc cgggcgcgcc
     4441 ctccaaaaga accagcttct gggataaggt tccgtgatgc cgcttcccaa aaactcgcca
     4501 agtaaaacct aggtcatgaa tccccgactg tcaaatacgg agctaccagg cgttagtatc
     4561 acggccgatc agtgaatgga atagcgtgaa atcccgttga aagcatcaga aaaggagtca
     4621 tttataggca gttcaataag tcagcagcga ccctatcttc gtccgaagct ccgtgcagca
     4681 tgggtggcga gaaagtgatt cgattacacg gcccagttgg gccttgactt cttcatcttt
     4741 tccttggaca gaaagctcgt tcttccattc ccggcagacg cggtaaagat ttgcctgaat
     4801 cggtcccgag tcgggacggc actcaagtgc caacatcaaa cgggtggcgg cttcggcaaa
     4861 ctctcgccgt tcagccaagg caatcgccca attgttaagg cttgccagaa gattgtcttg
     4921 tgccgactga ttctccggat ccaactggat ggctcgcagg ttgctaacaa ccgccacttc
     4981 atattgtcga tccaaaaggg atgcaacgcc ccggttgtaa tagattgtcc ccaaaagttg
     5041 tgtctcagaa atactccgcc ccgatttcgg atcgggggtc ccttccgatt gttgcgctcg
     5101 ggaaaccatg gggtgctcga gccgattgcc attgctcagc gttttgccaa aattttggcg
     5161 atcgacatct tgggctaatt ggtcccggtt ggagaggttg tcaaaccagg ttgggcaagt
     5221 acattcgaca gggacccaat tgtcttcaca ccttaccaag caataagcat gcgtcggcga
     5281 ttggagcact cgcacttcga aacccaacga gtcggcaacc aatttaaata ggattgcggc
     5341 actgacgcag ttgaaccggc ctgattggat cgcctcgccc ggattactgg cgccgataaa
     5401 atacccgccg tacagtattt ccgaatgcag agcccgaaag gcctgttgaa ggcgtacaag
     5461 cggcggcatg cggggatcca cccgtgcctg gattcgtttc atctgatccg caaactttcg
     5521 aaggagaaat t
//
LOCUS       DAOSUT010000221         5499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6693_length_5499_cov_9.449118, whole genome shotgun sequence.
ACCESSION   DAOSUT010000221 DAOSUT010000000
VERSION     DAOSUT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_5499_cov_9.449118"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLD05_14525"
     CDS             <1..949
                     /locus_tag="PLD05_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78702.1"
                     /translation="QALDELEEVMRDYQTDWANYLSELEDAKKQKRPAPKEPVRPDLE
                     KLAKEKNIDFVAVKDADVWTVAKLDIGKSYDLQGTPFVRSVFNLGEFITVRTSDTEQN
                     QYLCWLIAKTDERVPELDEPGVRENVIRAWRMAEARKIAESEIGAIVEKVNKEQKSLS
                     EFRAAHPDLSLPEVIQSEPFSWLTFEQLDLARSSNPQPRLGSIKVRTKDPASGLIVDK
                     DAVEGLSNDFMAAVFSLGVGETGKAWNRPQSIIYLVRVIEYQPPIEQLREMFFRQTDP
                     VYLAAGQIEFNEIAMRWIRGLEDDAGLKWNRPARRGARD"
     gene            complement(1093..1737)
                     /locus_tag="PLD05_14530"
     CDS             complement(1093..1737)
                     /locus_tag="PLD05_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOP78703.1"
                     /translation="MESSLQEPALTDEQLVEKARNGDFEAFETLVNRYERRVFLLALR
                     IVRRSHDAEEVVQKTFLSVLEHLSDFRGDSAFATWLLKIATNHALGLLRREALRTTLP
                     LAGGSHDVEDDDGLPKPRYIAEWRDSPDEIVSRKETRDLVEQALQELDEKYRLVFVLR
                     DVEGFSTAETAQILNLTEANVKVRLLRARLMLREKLTELFGDPERVLPPHSHEE"
     gene            2250..>3739
                     /locus_tag="PLD05_14535"
     CDS             2250..>3739
                     /locus_tag="PLD05_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOP78704.1"
                     /translation="MSIENLNKIFNPYRVAVIGASDTPTSVGYTVLKNLVGSGFRGVV
                     YPVNPKREAVQGIHAYKDVASLPHAPDLAVICTPARTVPGLVHELGEVGTRGIVIISA
                     GFREIGDEGKRLEAQILEEQAKYPGMRIIGPNCLGIIVPGISLNASFAAATPPKGHIG
                     FISQSGALCTSVLDWAIDQGIGFSYFVSVGNMLDVTIADLIDYFGSQTETQSIILYIE
                     SITEAREFMSAARAFARTKPIVAYKAGRFAESAAAAASHTGAMAGVDAVYEAAFQRAG
                     IERIFQIEDMFDCAELLARQQPPKGDRVAIVTNAGGPGVMTTDALIALGGHLAELSEE
                     SIKKLNEFLPPFWSHNNPVDVLGDAPPERYAKALEVVAEDPNVDAVFVILTPQAMTDS
                     TGTALAVAAAAKKHKKPVLAAWMGGHTVAEGMQILSENNVPCYTTPEKAVRAFMHLVS
                     YARNLTLLHETPRDIPVSFSFDRQRLRGVFESLLTEGAEILTESVS"
     assembly_gap    3740..3839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3840..4739
                     /locus_tag="PLD05_14540"
     CDS             <3840..4739
                     /locus_tag="PLD05_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOP78705.1"
                     /translation="IGTKRDPVFGSVILVGMGGVAAEVFQDRALALPPLNETLARRAL
                     ESLKSWPLLRGYRGRPGVNIDKLIEVLMRFSYLVADYPEIKELDVNPLLATAEEVIAL
                     DARVVVDRDILAHGVRPYAHLAIRPYPEEFVTERKLRDGTPVILRPIKPEDEPMWHEL
                     LASCSTQSIWFRFSYLFKHTTHEMASRYCFIDYDREMGIVAEVEEDGQRRLIGVGRLV
                     ADANHETAEYAVIVADRWHGRGLGGLLTDYCLEVARRWGIKKVVAETSKDNARMLATF
                     RNRGFEFNEKADQDVVLVSKTLA"
     gene            complement(4819..>5499)
                     /locus_tag="PLD05_14545"
     CDS             complement(4819..>5499)
                     /locus_tag="PLD05_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78706.1"
                     /translation="DDSSPVGNAKPVLSREPAQQETELLTRRRHPRLAAAEAQGRWQP
                     LEIALPPGMADKQSEPKPSPSPGDVYPIAEWPPVSPDQSKGTGSQSQEAGSVRSVLIP
                     RRPRVTREALLEGSPECSAPPTGTASVKSAEHPLDMVVCPPTPAGQQDGRGQSPPCVA
                     TAEPTIRETPPTSSRGPVRVLLPFGLTVPRWLIAALGFVISALLGLGLGYWIIVNFIP
                     NTGLLKLW"
BASE COUNT         1190 a         1389 c         1602 g         1218 t
ORIGIN      
        1 gcaagccctt gatgagctcg aagaggtgat gcgggattat caaacggatt gggccaatta
       61 tctgagcgaa ttggaagatg cgaagaagca aaagcgtccg gctcccaaag aaccagttcg
      121 gcctgatctg gagaagctcg ccaaggaaaa gaatatcgat tttgttgcag tcaaggatgc
      181 cgatgtttgg acagtggcga aactggatat cggaaagtcg tacgatttgc aggggacgcc
      241 attcgtacgg tccgtcttca atctggggga gttcatcacc gtacggacgt ccgataccga
      301 gcaaaaccag tacctttgtt ggcttattgc gaagactgac gagcgcgtgc cagaactcga
      361 tgagccgggg gtgcgggaga acgtcattcg ggcatggcga atggctgagg cacgaaaaat
      421 tgcagaaagc gaaatagggg caattgtcga gaaggtcaat aaggaacaaa aatccctgag
      481 cgaatttcga gccgctcatc cagacttgag tttgccagaa gtgattcaat cagaaccgtt
      541 cagttggcta acttttgagc aattggattt ggctcggtcc tcaaaccctc aaccgcggtt
      601 gggcagcatc aaagtgcgta ccaaggatcc ggcaagcggg ttgattgtgg ataaggacgc
      661 cgttgaaggg ctcagcaacg atttcatggc agcagtcttt tccttgggcg ttggggagac
      721 gggcaaagcc tggaaccgac cgcaatcgat catctatttg gtccgggtaa ttgagtatca
      781 acctccgatt gagcaattgc gggaaatgtt cttccggcaa actgacccag tttacttggc
      841 ggcagggcag atcgagttca acgagatcgc gatgcgatgg attcgcgggc tagaagacga
      901 cgccggattg aaatggaatc ggcctgcccg gcgcggtgcc cgggattaac tcggataaga
      961 ctgttgccgt ttcaatgtga cgacacggca gagccgaggt ttccgatgaa ggtcgccaag
     1021 gcggggtgac tgcatgggca gtacggctgt atctgagcca aggcttttgc ctggcgatgg
     1081 aaaagacgaa gtttactcct cgtgggagtg aggaggaagg acgcgctctg ggtcaccaaa
     1141 cagctccgtg agcttttccc gcaacatcaa gcgtgcccga agaagtcgga ctttgacgtt
     1201 cgcctcggtc aaattaagga tttgggcagt ttcggctgtg gaaaacccct ccacatcacg
     1261 cagcacaaat accagacggt atttttcatc caactcctgg agggcctgct cgaccaaatc
     1321 gcgcgtctcc ttgcgagaga caatctcatc cggagagtca cgccattccg caatgtagcg
     1381 gggcttgggt aatccatcgt cgtcctcaac gtcgtgggaa ccaccagcaa gaggcagggt
     1441 cgtacggagg gcctcccgtc gaagcagccc gagcgcgtgg ttggtggcga tcttcaagag
     1501 ccaggtggca aatgccgagt caccgcgaaa gtccgaaagg tgttcaagaa cacttaggaa
     1561 ggttttctga acaacctctt ccgcatcgtg ggaccgacgc acgattcgta gggcgagcaa
     1621 gaacacacgg cgttcgtagc gattgacgag ggtctcaaat gcctcgaaat caccgtttcg
     1681 cgccttttcg acaagctgtt cgtcggtcag tgctggttct tgtagtgacg attccatgaa
     1741 atttttatca caattgccgc tgtcatccca cggttacaaa ggacctgtcc cttagtcccc
     1801 tgcggtacaa tctggatttt cggagatctt tcagaccacg ggctatggaa ccactggcga
     1861 ggtccttcat tatccaggtt ttgcggcttt cgacaaccgc ggcatgatgg aatctgtagg
     1921 aagtggaaac tttcggatga aatcccccgt aacaaaagta gagctgacaa gtaggttttt
     1981 gtgatttgtg acagttttag taagccatat tgttccttgc gttcgcttaa ggtcgcctag
     2041 ccgtttcatc ccgcacaatc gtttcatgct tggcaaagcg cggcattttt cccgcccgtc
     2101 aatctttgcg ccactttcga cgatgacaca gatgggctat tttaaaagtt gagccgttcg
     2161 cttcgcagaa caatctcggc ttgcctgagg ccgattcatg atggggtttt gtttgccata
     2221 aaacgagctt aacgcaacag gagcctgccg tgagtattga aaacctgaac aagattttta
     2281 acccgtatcg tgtcgccgtg attggggcga gcgacactcc gacaagtgtc ggttataccg
     2341 ttctcaagaa tcttgtcggc tccggatttc ggggagttgt ctatcctgtc aatccaaagc
     2401 gagaggccgt ccaaggtatc catgcatata aagatgtcgc aagcctgccg catgcccccg
     2461 atttggcggt gatttgtacg ccggcacgaa cggtgcccgg tttggtccat gagttgggcg
     2521 aagtgggaac gcgtgggatt gtcatcattt ccgccggttt ccgggaaatt ggggatgagg
     2581 ggaagcggct tgaggctcaa attttggagg aacaggccaa gtatcccggc atgcggatca
     2641 ttggtccgaa ctgtctgggg atcatcgttc ctggcatcag tttgaacgcc agctttgccg
     2701 cagccacgcc cccgaaggga catattgggt ttatctcgca gtctggtgcg ttgtgcacgt
     2761 ctgtcctcga ttgggccatt gaccagggga ttggattctc ctatttcgtc tccgtgggca
     2821 acatgctcga tgtcaccatc gccgatttga tcgactattt cggatcgcag acggagacgc
     2881 agtccatcat tctttacatt gaatccatca cagaggcgcg ggaattcatg tcggcggccc
     2941 gtgcctttgc ccggaccaag ccgattgttg cctacaaggc cgggcggttt gcggaatccg
     3001 ctgccgcggc tgcttcccat accggggcca tggcaggtgt ggatgccgtg tacgaggcgg
     3061 ccttccagcg ggcggggatc gagcgaatct tccaaatcga ggacatgttc gattgtgccg
     3121 agttgctggc acgccagcag ccgcccaagg gtgaccgcgt ggccattgtg acgaatgctg
     3181 gcggacctgg cgtgatgacg accgacgccc tgatcgccct tggtggccac ttggcggaac
     3241 tctcggaaga gtcgatcaaa aaactgaacg aatttctccc tccattctgg tcgcacaata
     3301 accccgtaga tgtccttggg gatgccccac cggaacggta tgccaaggcg ttagaggtcg
     3361 ttgcggaaga ccccaatgta gatgcggttt ttgtgatttt gactcctcag gcaatgaccg
     3421 attccaccgg gacagcgtta gccgtggcag cggccgcaaa gaaacacaag aagccagtct
     3481 tggctgcctg gatgggagga cacacggtcg cggagggcat gcaaatcctt agcgaaaaca
     3541 atgttccctg ctacacgaca ccagagaaag ccgttcgcgc gtttatgcac cttgtctcat
     3601 atgcccgcaa tctcacgttg cttcacgaaa cgccgcgaga cattcccgtt tcgttctcgt
     3661 ttgatcgcca acgcctccgc ggggttttcg agagcctgct caccgaaggt gcggaaatcc
     3721 tcacggaaag cgtttccaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     3841 ttgggacgaa gcgagatcct gttttcggtt ctgtgatcct ggtcggaatg ggcggggtcg
     3901 ctgcggaggt cttccaagac cgggcattgg cccttccgcc cctcaatgaa acgttggctc
     3961 ggcgtgccct ggagtcgctc aagtcgtggc cgctattgcg aggttatcgc ggtcggcctg
     4021 gcgtcaacat cgacaagtta atcgaagtgc tcatgcggtt ctcgtatctg gtggccgact
     4081 atcccgagat caaagaactg gatgtaaatc cgctgttggc caccgcagaa gaggttattg
     4141 cgttggacgc gcgtgttgtg gttgatcggg atattttggc ccacggtgtt cgaccgtatg
     4201 cccatctggc aattcggcca tatcccgaag agttcgttac ggaacgaaaa ctcagagatg
     4261 gtacgccggt gatcctccgg cccatcaagc ccgaagacga gcccatgtgg catgaattgc
     4321 tggcgagctg ttccacgcag tcgatttggt tcagattcag ttacctgttc aagcacacga
     4381 cacatgaaat ggcgtccagg tactgcttta tcgactacga ccgagagatg ggcatcgtgg
     4441 ccgaggtcga agaggatggg cagcgccggc ttatcggtgt ggggcgtctc gtggccgatg
     4501 ccaatcacga gacagcagag tacgctgtga tcgttgccga ccgctggcac ggtcgaggtc
     4561 tcggcggcct gctcacggac tattgcctgg aggtcgccag gcgatggggt atcaagaagg
     4621 tcgtggcaga aacctcgaag gacaatgccc gaatgttagc caccttccgg aaccgtggat
     4681 tcgagttcaa tgaaaaagcc gaccaagacg tggtgctcgt ttccaagaca ttggcatagt
     4741 aggctacgag ttcaccgctg attatcgaca gtaaccgcac agcccacgtt gtgggctgtg
     4801 tttttttgtg gtcatggact accaaagctt gagtagccca gtgtttggga tgaaattgac
     4861 aatgatccaa tagcccagtc ccagacctaa caaggcagaa atgacaaagc caagggcggc
     4921 gatgagccat cgagggacag ttaagccgaa cgggaggagg acacgcaccg gaccgcggga
     4981 tgacgttggc ggtgtctccc ggattgttgg ctcggccgtc gcaacgcatg gcggggattg
     5041 tccccgacca tcctgctggc cagcaggtgt gggtgggcag acaaccatgt cgagagggtg
     5101 ttccgcgctc ttgacgctcg ccgtgcccgt gggcggcgcg ctacattcgg gtgaaccttc
     5161 aagaagggct tcgcgtgtta ccctaggtct gcggggaatc aatacacttc tgacagagcc
     5221 cgcttcctgg gattgagatc ctgtgccttt ggattgatcg ggagaaactg gtggccactc
     5281 cgcaatcgga tagacgtcgc ctggcgatgg agaaggctta ggttccgact gcttgtccgc
     5341 cataccgggc ggtagtgcaa tctccagggg ctgccatcgc ccttgggctt cggcggcggc
     5401 tagccgggga tgtctccgtc gtgtcaaaag ctcggtctcc tgttgggctg gctcacgact
     5461 cagcacgggt ttcgcgttcc ctacaggcga agaatcatc
//
LOCUS       DAOSUT010000222         5491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6711_length_5491_cov_8.743377, whole genome shotgun sequence.
ACCESSION   DAOSUT010000222 DAOSUT010000000
VERSION     DAOSUT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_5491_cov_8.743377"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLD05_14550"
     CDS             <1..776
                     /locus_tag="PLD05_14550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78707.1"
                     /translation="LKKRMIFCIVCYELGPIAAPMVPKLITLAEDNQDPCRRAAIGAM
                     VVIGPGAAPAVPILRKQLYHEDFHTQYWSCRALAAIGSPALPAVPDLVDRLKNGVASV
                     RRNAAAALGKLGPDIGPEAIAALIQAVDDPIQPVREQAVLALGQIGPQVAQQAREAIE
                     KSHENRPIFPKSAATWAIWRLGGPLEPLAESLVAEIKEGIYREEATAILREMGTAGEP
                     IQKRLKECLSEASETDSEEVANIQATLQELGAAQASQPN"
     assembly_gap    927..936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<937..1578)
                     /locus_tag="PLD05_14555"
     CDS             complement(<937..1578)
                     /locus_tag="PLD05_14555"
                     /inference="COORDINATES: protein motif:HMM:NF028343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOP78708.1"
                     /translation="MNFAVLFTAGWVLLPCLFEGVVPSDGDSVSSRHKITIIESHKDG
                     PTVLVIAGIHGNEPAGVEAAKTIAGWQILKGRVIVLVEANPAAIAAQKRLIPGAPVGE
                     GDLNRNFPVDEHGCHPVGDVATELWQVIKEHSPQWLVDLHESINFRRVDPQSVGNTII
                     VCPNEQTLAMGQKLVDAINKTITDESKKFLLLRPPAKGSLTRAAAETMNVNAIL"
     gene            2086..3210
                     /locus_tag="PLD05_14560"
     CDS             2086..3210
                     /locus_tag="PLD05_14560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOP78709.1"
                     /translation="MNHHVFAKVIAIVTASMLGILTGQVIGADLEHVVVFSAGEAGYH
                     TMRIPAIEVAGDGTILAFAEARKNSAADPGYPGQEIDLVMKTSPDGGRSWSDLVVLEH
                     VDELWSSANPAVVRDRTNGRIWLFYLQCAPGRSSHTARPGTLDVKNLVRFSDDNGRTW
                     SDAIDITSVARDLSDKEWGISVPGPGGAIQDRQGRLIIPMWRYRPFRDFVLFSEDHGK
                     TWHRGDFVPTQEGLDESQLVELADGTLALDMRQQRGSHRWLSFSRDGGRTWSEPRPGQ
                     EVTPVCCAVERWTLKSQGDDRDRIVWTGPRGPGRNNLTLWISYDELASFQNPRLIAEG
                     PGAYSDLTVLPDKSLGILWEQGNYKTITFTRVSLSWVEPR"
     gene            complement(3207..5348)
                     /locus_tag="PLD05_14565"
     CDS             complement(3207..5348)
                     /locus_tag="PLD05_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78710.1"
                     /translation="MANLRIFMQESFRGLVGVVLLSAILAAPCSMMTELAALGEMSKS
                     PVGENLIVNGDFEMEPMTSPPVGWVMWGAQRFKIAENYRPDLEICHSGRASLRVFHPQ
                     DTAGYIVTDPQMAIRPEMGMIYEISFFARADRPCRGRFMVLGYHSIRPFVDAESPLST
                     EISLGTDWQQYHFQIRESIDFFYDSARYLILGFQASVDPKVECIMWLDDVVATRKPME
                     PGTRLINPLSLKVSPLVHRLTPGPSLEVVADLGCPSRPALKAACGISFHRLNGFTGYP
                     YDATGQYVLPNPLEKAIRELRLPMSRFYGVGHEPARSDLPPWPVEVAIDRLAELCDRL
                     QIPQEAMVIELEEQSANRKLDAEVWAKAVQHARSKGYRFRYWEVANEPYSQTFGSQQP
                     MGRAFATSDDYIAHVKSVATAIRMIDPQAQVGVAIAISRPLWGNYVLKHAAEYYDFVV
                     AHYYGFENAYRSSFEELVLSQNYRILERIARCQALIDAYNPGRHVYQLDTEWGMHSSG
                     PEGERADYALRNANIIGVVHRAVRMIYYVREQPLAGASSWQMLGHSRGLGFTVLATDR
                     PDSRTMLYWLYYYFLRHVGDNLVDFVGTAPFYPAIDRKTEDATTGPVTPVLLTTDTRQ
                     QCVFCIIANGSWSNDVPCRFLLKNYPGRLVSKVLLSDDDLDRNPLLNTKDGFVRDIQM
                     NVEVDRQSKSTIVTGTIPARSVVFLTLERLP"
BASE COUNT         1276 a         1484 c         1484 g         1237 t
ORIGIN      
        1 atcttaagaa gcgcatgatc ttctgtatcg tttgctacga attgggaccg attgctgcgc
       61 ccatggtgcc gaagttgatc acgttggcag aggacaacca agacccatgt cggcgggcgg
      121 cgattggcgc catggtggtc atcggtcccg gcgctgcacc cgctgttcca atcctgagaa
      181 agcaactgta tcacgaggat tttcacacgc aatactggtc gtgtcgagct ttggcggcaa
      241 tcggctcgcc ggccctgccg gccgtgccag acctcgtcga tcgacttaaa aacggtgtgg
      301 catccgtgcg acgaaacgcg gcagctgccc taggtaaact gggtcctgac attggccccg
      361 aggctattgc ggcgcttatc caagcggttg acgatccaat tcaacccgtc agagagcagg
      421 cggtcctcgc tcttgggcaa attggtcccc aggttgccca acaagcccgc gaggcaattg
      481 agaaatccca tgagaaccgc ccaattttcc caaagtcggc ggcgacctgg gccatctggc
      541 gactcggcgg gccgcttgaa cctcttgccg aaagtttggt tgcggaaatc aaagaaggta
      601 tttaccgcga agaggcaacg gccattttgc gcgaaatggg gacggcaggc gagcctattc
      661 aaaaacgcct taaagaatgc ttatccgagg catccgaaac ggactctgag gaagtggcca
      721 atattcaggc aactttgcaa gagctcggtg cagcccaagc aagccaaccc aattgatcgt
      781 atggagacga ttcattctcg ccggggaaaa ttatcattgc tgttcttcct ccggttccag
      841 catgcccagt tctcggaaga atctttcgag gagaaggcaa tgctgcttta cacgcgtctc
      901 aagcggctct cggcgggtcg tttcaannnn nnnnnncagg atcgcgttga cgttcatagt
      961 ctcggccgcg gcccgcgtga ggctcccctt tgccggcggt cgcaggagga ggaatttctt
     1021 tgactcatcg gtgatcgtct tgttgatggc atcaacaagc ttttggccca tggctaaagt
     1081 ttgctcgttt gggcaaacga taatggtgtt accgacactt tggggatcga cgcgtcgaaa
     1141 attgatcgat tcgtggagat caaccagcca ctgaggagag tgttccttaa tcacctgcca
     1201 aagttccgtg gcaacatctc caacgggatg gcaaccgtgc tcatcaactg ggaaatttcg
     1261 attgaggtcc ccctcaccta caggggcacc agggatcagt cgtttttggg ccgcgattgc
     1321 tgccggattc gcttcaacca gaacgataac ccgccccttg agaatttgcc acccagctat
     1381 tgtcttcgcg gcttccactc ccgcgggctc gttaccgtga attccagcaa tgactaaaac
     1441 cgtgggacca tccttgtgag actcgataat cgtgatcttg tgacgacttg acacagaatc
     1501 accgtccgag ggcacaacac cctcaaaaag acagggcaac agtacccagc cggctgtaaa
     1561 tagcacagca aaattcataa agttatcatc tcgaatgacg atccgaccca gtaattatca
     1621 gtggtgaaaa tataatccat ctcgaaagcc ctgacaagag caccaatatg cccgaaagac
     1681 accgtccggc ctggccttcg ccatcattta tgcaccactg agaagctgag ttgaggcccg
     1741 gtcttgtgat tgcaagagcg aactttgtgg gcgcgaattt tggcaaagaa agcaaggtca
     1801 ctaaatctta cctggacgcc catggatggc tgacgaagtg aacgcatcgt tggccatctg
     1861 ttttgttgtt ttgatgcacg ccgaaccacc gcccgctgaa gcacgttctt ccaaaagatg
     1921 tgcacaccgt gtgcttaaaa tggtttccct ttcgagttag accgacgcga aaagttgccg
     1981 tggcggattg cttctgtgag cagtaggatt atcatgtttt tggcaaagta ccataagaca
     2041 taagtgaggt gacccgtttt gcctggattc gtgaaaggta cctccatgaa ccaccacgtt
     2101 ttcgcgaagg ttatcgcaat cgtcaccgcg tcaatgctcg gtattttaac cggtcaagtg
     2161 attggggcag acctagaaca tgttgttgtg ttctccgcag gtgaagccgg gtatcacacg
     2221 atgaggattc ccgcaattga ggtggcaggt gacggaacaa tcttggcttt tgcggaagct
     2281 agaaagaaca gcgccgctga tccgggttat cctgggcaag agatcgatct tgtaatgaaa
     2341 acgagccccg acggtggccg ttcttggtca gatttggttg tcttagagca cgttgatgaa
     2401 ctttggtcgt cagcgaaccc ggccgtcgta cgcgaccgaa ccaacggccg gatttggctc
     2461 ttttacctcc aatgcgcccc tggaaggagt agtcatacgg cgcgtccggg aactctggac
     2521 gtcaaaaact tggtgcggtt tagtgacgac aacggccgca cttggtcaga tgcgattgat
     2581 ataacaagcg ttgcacggga tttgagtgac aaggaatggg gaattagtgt gccagggcca
     2641 ggcggagcga tccaggatcg ccaggggcga ctaattatcc ccatgtggcg atatcggccc
     2701 tttcgagact ttgtcctttt cagtgaagac cacggtaaga catggcatcg cggtgatttt
     2761 gtgccgaccc aagaggggct cgatgaatcc cagctcgtgg agttagccga tggcaccctc
     2821 gctttggaca tgcgtcaaca gcgtggttcc caccgctggc taagctttag ccgtgacggc
     2881 gggcgaactt ggtccgaacc acgaccgggt caagaggtaa cacccgtttg ctgcgctgtc
     2941 gagcggtgga ccctcaagag ccaaggggac gatcgggacc gcattgtctg gaccggtccc
     3001 cgtggccctg gacgcaacaa tctcacactt tggatcagtt acgatgagtt ggcctctttt
     3061 cagaaccccc gtttgatcgc cgagggacct ggggcgtact ccgatctgac ggtgcttccc
     3121 gataagagtt tgggaatcct gtgggagcag ggtaactaca agacaattac ctttacccgt
     3181 gtgagccttt cctgggtcga accgcgttaa ggaagtctct ccagggtcag gaagacgaca
     3241 ctacgggcgg gaattgtccc ggtcactatg gtacttttgc tttgcctatc gacttccacg
     3301 ttcatttgga tatcacgtac gaaaccatct tttgtattta gcaaagggtt acgatccaag
     3361 tcgtcatcgg agagaaggac ttttgagacc agtcttcccg gatagttctt gagaaggaaa
     3421 cgacacggca catcattcga ccatgatcca ttggcgataa tgcaaaatac acactgctgc
     3481 cgagtatcag ttgtcagcaa cacgggcgta acaggacctg tcgttgcgtc ttcggtcttc
     3541 cggtcaatcg ccgggtaaaa aggggcagtt cccacaaagt caacaagatt gtctccaaca
     3601 tgccgcagga agtaatagta gagccagtat aacatcgtcc gcgaatctgg tcggtccgtc
     3661 gccaacaccg tgaaacctaa cccccgtgaa tggccaagca tttgccacga ggacgcaccg
     3721 gcaagcggct gttcgcggac gtagtaaatc atgcggaccg cacggtggac tactccgatg
     3781 atattcgcat tgcggagggc atagtccgcg cgttcacctt ccgggccgga tgagtgcata
     3841 ccccattcgg tatcgagctg gtacacgtgc cgccctggat tgtacgcatc tatcagggcc
     3901 tgacaacgag cgattcgctc gagaatacgg tagttttggc ttaggacgag ctcttcgaac
     3961 gagctacggt aggcattttc aaaaccgtag taatgggcca cgacaaaatc gtaatattct
     4021 gctgcatgtt ttagcacgta attaccccac aacggtcgcg atatcgcaat agcaacaccg
     4081 acttgggctt ggggatctat cattcggatg gccgttgcca cggacttcac atgggcgatg
     4141 taatcgtcag acgtagcgaa cgccctaccc atgggctgct gcgaaccgaa cgtttgcgaa
     4201 tacggctcgt tggccacctc ccaatatcga aagcggtagc ctttgcttcg agcatgttga
     4261 accgctttag cccaaacttc agcgtccagt ttccgattgg cactctgctc ctccagctca
     4321 atgaccatcg cctcttgagg gatctgtaga cgatcacaaa gttctgcaag gcgatcgatc
     4381 gccacctcca ccggccaggg cggcagatcg ctcctcgccg gttcgtggcc aaccccgtag
     4441 aagcggctca tcggcaggcg tagttctcgg atcgccttct ccaagggatt aggtaaaacg
     4501 tattgcccgg tcgcatcgta tgggtacccc gtaaagccgt tcaatcggtg gaacgatatt
     4561 ccgcaggccg ccttcagtgc cggtcgagat ggacacccca aatcggctac gacctcaagg
     4621 ctggggccgg gcgttagccg atggaccagc ggcgaaacct ttagagaaag tggattgatc
     4681 aagcgcgtgc ctggctccat cggctttcgc gtagcgacca cgtcatcgag ccacataatg
     4741 cactcaactt ttggatcaac cgacgcctga aagccgagga tcaagtaacg agccgaatcg
     4801 tagaagaaat cgatactttc ccgaatttga aaatgatatt gttgccagtc tgtgccaagg
     4861 gaaatctctg tgctcaatgg ggattcggca tcgacaaagg ggcgaatcga gtggtatccc
     4921 agtaccatga atcgccctcg acatggccta tcggcgcggg cgaagaaact gatctcgtag
     4981 atcattccca tttccgggcg aatcgccatt tgcgggtccg taacaatgta ccctgccgtg
     5041 tcttggggat gaaaaaccct caagcttgct cggccactgt ggcagatctc gagatcaggt
     5101 cgatagttct cggcgatttt gaaccgttgc gcgccccaca ttacccagcc gacgggcggg
     5161 ctggtcatcg gctccatctc aaagtcacca ttgacgatca ggttctcacc gaccggcgac
     5221 ttactcattt caccgagtgc cgcgagttcg gtcatcattg aacagggcgc ggcaagaatc
     5281 gcactcagca agaccacgcc gaccaagccg cgaaaagact cttgcatgaa tatccttaga
     5341 tttgccattt acctctcctc cagagctcca acctcttcgg taataaaacc agattacttt
     5401 catccgatag gccgctacga gcctatccca taatctcccc tcgcccgttc ggcgggagag
     5461 gggtcggggg tgagggtggc tcgattcgct g
//
LOCUS       DAOSUT010000223         5487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6715_length_5487_cov_10.318667, whole genome shotgun sequence.
ACCESSION   DAOSUT010000223 DAOSUT010000000
VERSION     DAOSUT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_5487_cov_10.318667"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="PLD05_14570"
     CDS             complement(<1..1457)
                     /locus_tag="PLD05_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP78711.1"
                     /translation="MLRPRFHVLSAIVLLVTIFALPAGASDFQAEQGNLPHIVLILAD
                     DLGYGDLSCLNPDSKIQTPNLDQLAQEGMIFTDAHTPSSVCTPTRYGLLTGRYSWRTR
                     LQSGVLYGFDRPLLEPNRMTLASLLKSHGYATAAVGKWHLGLGWQSAQGTPVTDKDFT
                     TDDPGVDYTRPITEGPLTVGFDYFFGIAASLDMAPYCYIENDRVTAIPQEDTKGQEFP
                     MNWRPGKKSPDFKHDQVLSVLTERAMGWLSKTHKKAPKQPLFLYFALTAPHTPVLPLP
                     EFQGKSQAGSYGDFVVQVDHVVGQLVKTIEDLGIRDNTLVIFTSDNGSTMTIRPFFQK
                     YQHATNYHFRGQKSDIWEGGHRVPFLVRWPGHVPPKSVCHQPICLTDCLATFAELVSS
                     PLPDDAGEDSFSFLDLLENPNAPFQRAPIVMHSIAGFFAIREGDWKLLECRGSGGWSL
                     PENKVPKDALPKQLYNLKDDVGEQHNLLLEHPDVA"
     gene            complement(1656..>2117)
                     /locus_tag="PLD05_14575"
     CDS             complement(1656..>2117)
                     /locus_tag="PLD05_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78712.1"
                     /translation="TAVAADDMMQAFYYHHLVPAGTWAINVMGRSRYGIAWVLPPEKA
                     IRIPIADKAEIRLPWIWIGSSAASLKLELTDAPEGFAVVSLERDGGGAKLILSVDKDK
                     IKPGTKGNLVFAVYGETQRRGLVAASTQTVRYFVGWLPAVPFVAVEQKSSS"
     assembly_gap    2118..2217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2218..3755)
                     /locus_tag="PLD05_14580"
     CDS             complement(<2218..3755)
                     /locus_tag="PLD05_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOP78713.1"
                     /translation="MKRHQTLLSLGILVAFWSFWTPVQGQQVPSSMRLPHVGYVYPAG
                     GQAGTEFDVTIGGQYLDGISEVLISRLDPLGSMESGVPRGTFVKLSKPLTQKQVNDLS
                     EKLKDIQARVREEMRRRRLSVGQYRELFVKFAAEAGITEEMLKELDEFRKNRNDPKRQ
                     PAPQIAERATIHIVLPKDTEPGLYLIRLRTRTGVSEPLRFQVTPWQEVLENEPNDHGQ
                     VHTVTEGLPVVLNGQIMSGDVDRFQISAKKGQHLVVAVAARELIPYLADGVPGWFQAT
                     LRVLDDSGREVAYVDDFWFRPDPALSFEVPRDGDYIIEVKDAIYRGRQDFVYRIVVGE
                     IPFVTSIFPLGGRLGEKCQVTLNGWNLPQREWTLAPLKGDPGIRQILLPQWAAMPRSV
                     SFAVGQWPEVLEQEPNDGRANAQKLITGVVVNGHIDKPDDRDVFVIEGRAGQNIVAEV
                     LARRLYSPVDSILRIIDENGQGVAVNDDTEDKSAGLLTHYADSFVQVKLPKDGKYFIE
                     LTDG"
     gene            complement(3884..5287)
                     /locus_tag="PLD05_14585"
     CDS             complement(3884..5287)
                     /locus_tag="PLD05_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOP78714.1"
                     /translation="MSDFRLAHVILNPADQAGPREKLTRRTLLARGLFGVLGLYLGHR
                     GFTSPSAWAFDPLLPADKPGKAKAVIQIWLWGGPSHLDTFDPKPDAGNDYTGPFTNPI
                     STNVPGIRINELLTELAKVADKYSLIRGMTHGVNAHETAAYLVQTGRMPGDRIVAPAV
                     GAVVSYFKGQPPEYQSLIPPYIVLTEPQGRFSEAGFLGPKYKPFATGGDPSKTPFTVE
                     GIVAPGMTQSRQRERRELLAKLDVLRQTVGDHPQIEELKQAEEQAYDLILGSSGKAFD
                     PSEEPQELRQKYGTTTFGQSCLVARRLVERGVRYVTINSKGWDTHKEHFQAMRRKLPD
                     LDRGLATLIQDLADRGLLESTLIWCCGEFGRTPKIAWEPPWNGGRHHYGKAFSVLVAG
                     GGFKGGQVVGATDKTGSEVVERPVYPTDLIGSIYTLLGIDPATTVRHPQGQMVRIVPT
                     PEEGAPSAGLLKEIMTV"
     gene            complement(5303..>5487)
                     /locus_tag="PLD05_14590"
     CDS             complement(5303..>5487)
                     /locus_tag="PLD05_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78715.1"
                     /translation="SGNPRAKVTRLYLTVLSRYPTEKELAVIAETAKAQRSLSRQAGV
                     DLVWALINSSEFLYRH"
BASE COUNT         1214 a         1560 c         1431 g         1182 t
ORIGIN      
        1 tgggccacgt cgggatgttc gagcaggagg ttgtgttgtt cccctacgtc gtccttgaga
       61 ttgtatagtt gctttggcaa ggcatctttg gggactttat tctcaggcaa gctccagccg
      121 cctgagccac ggcattcgag gagtttccaa tctccttcgc gaatggcaaa aaagcccgcg
      181 atcgagtgca tcacaattgg cgcacgctgg aagggcgcat tggggttttc gagcaaatcg
      241 agaaaactaa aactgtcttc tcctgcgtca tctggaagcg gcgaggacac aagttcggca
      301 aacgtggcaa ggcaatcggt gaggcaaatc ggctgatggc agacactctt agggggaaca
      361 tgacctggcc agcggacaag aaacggtacg cggtggcctc cttcccaaat gtccgacttt
      421 tgtccacgaa aatggtagtt ggtggcatgc tgatattttt gaaagaacgg gcgaatggtc
      481 atcgtggaac cattatcgct ggtgaagatg accagtgtgt tatcacgtat ccctaaatcc
      541 tcaatcgtct tcacaagctg gcccacgacg tgatccactt gaacgacaaa gtctccgtaa
      601 ctaccggctt gactctttcc ttggaactcc ggcagcggca acacaggcgt gtgcggggcc
      661 gtcagggcga agtaaaggaa aagaggttgc ttgggcgctt tcttgtgtgt cttactgagc
      721 cagcccatgg ccctttctgt cagaacagat aggacctgat cgtgcttaaa atcgggcgat
      781 ttcttgccag ggcgccagtt catcggaaac tcttgccctt tcgtgtcttc ctgcgggatg
      841 gcagtaacgc ggtcattttc gatgtagcag tagggcgcca tgtcgagaga agcagcgatc
      901 ccaaagaaat aatcaaaacc gaccgttagc ggtccttctg tgataggacg cgtgtaatct
      961 acgcccggat cgtcggtcgt aaaatcctta tcggtgacag gagtcccctg ggccgattgc
     1021 cagccaagcc ccaaatgcca tttaccgacc gctgccgtcg cgtatccgtg cgattttaag
     1081 agcgacgcca aggtcatgcg gttcggttcg agcaaaggtc gatcaaagcc gtacaagact
     1141 ccggattgaa gtcgcgttcg ccatgaatat ctccccgtca atagcccata tcgggtcggc
     1201 gtgcacaccg aggaaggcgt gtgggcgtcg gtgaaaatca tgccctcctg cgccagttga
     1261 tccaagttcg gtgtctgaat cttagagtcc ggattcaaac aggagagatc accgtagccc
     1321 aaatcgtctg ccaaaataag gacaatatga ggcaaattgc cctgctcggc ttggaagtca
     1381 ctcgccccgg cgggaagagc aaagattgtg accaaaagga caatggcact caaaacgtgg
     1441 aatcgtgggc gcagcatttt cggcctcctc tagctagttt gctcttgccc aaatcaatcg
     1501 tttgcgaata acgcggctag tcctttccct gggtgaggca ttcgctccta cagtaggaaa
     1561 tccaaggaca tttcctacag gccgaatcgc cattttacaa tcggagaccg gcaggaacaa
     1621 acgtatcgaa tcccgcccag cttaggaagc acccctcaag aactggactt ctgctcgacc
     1681 gcaacaaacg gcaccgccgg tagccatcct acgaaatacc gaaccgtttg cgtgctcgcg
     1741 gctacgagcc ctcggcgttg ggtttcgcca tagactgcga agacaagatt gccctttgtc
     1801 cccggtttga ttttgtcctt atccactgaa aggatcaact tggctccgcc accatctcgc
     1861 tccagactga cgactgcaaa cccttctggg gcatcggtga gttccaattt taggctagcc
     1921 gcggaactcc cgatccagat ccagggaaga cgaatctcgg ccttgtccgc aatcgggatt
     1981 ctgatggcct tctccggcgg caatacccag gcgatgccat accgggagcg gcccatcaca
     2041 ttgatcgccc acgttccggc aggcaccagg tggtgataat aaaaggcctg catcatatcg
     2101 tccgcagcaa ccgccgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc
     2221 ccatcggtca actcaataaa gtacttgcca tctttaggaa gcttgacctg gacgaatgaa
     2281 tcggcgtaat gggtgagcag tccggcgctc ttgtcttccg tatcatcgtt gacagctaca
     2341 ccctgcccat tctcgtcgat aatgcggagg atcgaatcca ccggtgaata gagacgccgg
     2401 gcaaggactt cagccacgat gttttgacca gcgcgcccct cgattacgaa aacgtcgcga
     2461 tcgtcgggtt tgtcaatatg tccattgacc accacgccgg tgatcagttt ttgggcgtta
     2521 gcgcgcccgt cattgggctc ttgctccaaa acttcaggcc attggcccac ggcgaaagac
     2581 accgagcgag gcatcgctgc ccattgggga agaagaattt gtcttatgcc gggatcgccc
     2641 ttgagcggcg caagtgtcca ctcccgctga ggcaggttcc acccgttcag agtgacttga
     2701 catttctccc ctaaacggcc cccgagtggg aaaatactgg tgacaaaggg aatctctccc
     2761 acaacaatcc ggtacacaaa atcctgacgg ccacgataga tggcatcttt gacttcaatg
     2821 atgtagtccc catctcgtgg gacctcaaag ctcagtgccg gatcgggacg gaaccaaaaa
     2881 tcatcgacat aggccacttc ccgaccggaa tcatcgagca cacggagggt cgcctgaaac
     2941 catcccggca caccgtcggc aagatagggg attaactcgc gagcggccac ggccacgaca
     3001 agatgttgcc cctttttcgc cgagatttga aaccgatcca cgtcaccgga catgatctga
     3061 ccattgagaa cgaccggcaa gccctctgta accgtgtgca cctggccatg atcgttcggc
     3121 tcgttctcaa ggacttcctg ccacggggtt acctgaaacc gcagcggttc agacacgcct
     3181 gttcgggtgc gaagccgaat gagatacagc cccggctcgg tgtctttcgg aaggacgatg
     3241 tgaatggttg cccgttcggc aatttgcggt gccggctgcc gcttgggatc attgcggttt
     3301 tttcggaatt catcaagttc cttgagcatc tcctccgtaa tccccgcctc agcagcaaac
     3361 ttcacaaaga gctcccggta ttgtcctaca gataatcgcc gacgtcgcat ctcttcgcgg
     3421 acccgggctt ggatgtcctt aagcttctcc gaaagatcgt tgacctgttt ttgtgtcagc
     3481 ggcttggaca gcttgacaaa agttccccgg ggtaccccgc tttccattga accgagtggg
     3541 tcaaggcggg aaatgaggac ttccgaaatc ccgtcgagat actgcccacc aatggtgaca
     3601 tcaaactcgg tcccggcctg ccccccggca ggataaacgt accccacatg gggcaatcgc
     3661 atgctgcttg gaacctgttg cccctgaact ggcgtccaaa aactccaaaa ggcgaccaaa
     3721 atgcccaacg aaagaagagt ctgatgtcgt ttcatccgag acatgatcgc acctaatcaa
     3781 cgaaaacacg attttttccg gcactcgtcc ctacgcgccg gattccaacc cactattgac
     3841 cgcacttcac ccaacggatc ggattgctcg atgggttaaa ctgttaaact gtcataatct
     3901 ctttaagcaa cccggccgag ggagcacctt cttccggtgt tggcacaatc cgtaccatct
     3961 ggccctgggg atgtcgaacc gttgttgccg gatcgattcc cagcaacgtg tagatgctcc
     4021 cgatcagatc cgtcggatag acaggccgtt caacgacctc ggagcccgtc ttatcggttg
     4081 ccccgaccac ctgcccaccc ttgaagcctc cgccagccac gaggacggaa aaggccttgc
     4141 catagtgatg ccgtcctccg ttccagggcg gttcccaggc gatcttgggg gtccgtccaa
     4201 actcgccaca acaccaaatc aaggtgcttt ccaacagccc ccgatctgcc aaatcttgga
     4261 tcagcgttgc cagcccgcgg tcgagatcgg gcagcttgcg gcgcatcgct tggaagtgtt
     4321 ctttatgcgt atcccaacct ttggaattaa tggtgacata acggacgcca cgctcgacta
     4381 ggcggcgtgc caccaagcag gattggccaa atgtcgttgt cccgtacttt tgtctgagtt
     4441 cttgcggttc ttcgcttggg tcaaatgcct tgccactcga acccaaaatg agatcatagg
     4501 cctgctcttc cgcttgcttc agctcctcaa tctgtgggtg atcgccgacc gtctgccgca
     4561 agacatcgag tttcgccaac agctcgcgac gttcccgttg acggctctgt gtcataccag
     4621 gggccacgat cccctccacc gtgaacggag ttttcgaggg atcgcctccc gtcgcaaaag
     4681 gtttatattt cgggccaagg aacccagcct ccgaaaaccg accttgcggt tccgtaagga
     4741 cgatataagg cggaataagg ctttgatatt cgggcggttg tcccttgaag taactgacga
     4801 ccgccccaac cgccggggca acaatgcggt cgcccggcat gcggccagtc tgcaccaaat
     4861 aggccgccgt ctcatgggca ttaacgccgt gggtcatccc ccggatcaga ctatacttgt
     4921 cggcaacctt tgccaattcc gtgaggagct cgttgatccg gatgcctggc acgttcgtcg
     4981 agatagggtt cgtaaaggga cccgtgtaat cgttccccgc atcgggcttg ggatcaaacg
     5041 tgtcgagatg cgatgggccg ccccacagcc agatttgaat caccgctttg gccttgccgg
     5101 gtttatccgc gggaaggagc ggatcaaacg cccatgctga tggtgacgtg aaaccccgat
     5161 ggcctagata cagtcccaaa acgccgaaca gaccccgtgc caggagggtc cgccgggtca
     5221 gcttctcgcg aggtccagcc tgatctgctg ggttgaggat tacatgggcg agtcgaaagt
     5281 cgctcataca ccgcctccgt atttagtggc gataaaggaa ttcagaacta ttgattaagg
     5341 cccagacaag gtccaccccc gcctggcggg aaagactgcg ctgggccttc gccgtctccg
     5401 caatgacggc caactccttt tccgttgggt atcgtgagag aaccgtcagg tacagacgcg
     5461 taaccttggc ccgcggattt cccgaag
//
LOCUS       DAOSUT010000224         5364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6876_length_5364_cov_9.025240, whole genome shotgun sequence.
ACCESSION   DAOSUT010000224 DAOSUT010000000
VERSION     DAOSUT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_5364_cov_9.025240"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLD05_14595"
     CDS             <1..281
                     /locus_tag="PLD05_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78716.1"
                     /translation="AREKRAERKAGRRPRLETHPEIPGTVKEGLPELVEPTATANQIS
                     SEVTSSDLMATSASPVMEGLANMLNSPEGLATAIVLKEILDRPEARWH"
     gene            399..1319
                     /locus_tag="PLD05_14600"
     CDS             399..1319
                     /locus_tag="PLD05_14600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOP78717.1"
                     /translation="MSVPIANPDRTRIGWIGTGVMGSAMCGHLLKGGYRCTVYNRSRS
                     KAEALLAAGATWADSPKRVAEVSDIVFTIVGYPNDVREVILGPDGVLAGLPTNGVVVD
                     MTTSEPSLAIEIAKAAAARGCHALDAPVSGGDVGAREARLSIMIGGEPDIVAAVMPCF
                     QLMGKTIVHHGGPGAGQHAKMTNQILIASTMIGVCEGLLYAYRAGLDLEKVIESVASG
                     AAGSWSISNLGPRIIQNRFEPGFYVEHLIKDLGIAIKEAKQMGLVLPGLALAEQLYLA
                     VAACGHARSGTHALQIALARLSGIDWLNRK"
     gene            1374..2765
                     /locus_tag="PLD05_14605"
     CDS             1374..2765
                     /locus_tag="PLD05_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78718.1"
                     /translation="MLRKIVIAQFLFVLGLAWPEVRAWGPHPQITKAALEVLPHIEEV
                     KAQLGEDNVMALTSYCWMPDMRGRDLGTFYADDYLLIRALPRHIGHTMPAVSAAFEPY
                     FRRALQALRTESPANACRQLGTLVHFVEDVGAPPHAKENCPHHSELENWVDAQLITIQ
                     GYQPRLLGQSDEEALQGLQARIAELVAFSKERAERALPIVSSPNPDRSKVEEIILESA
                     IESAKVTADLLFTMFTLGLRPNSGPGQSTLRGTITAPAAPGNDDQSPRVVLLNTDYST
                     LAEATSIGSSGGWQGKFVFRQLPAGKYQLFVYRIGAQPAILPVELRDGEVTHLTIELI
                     PSDPQGNLVYNPDGKTRVLVPDLPDRWSMSTSEKAKSWSSTVVPLKAGEKYRCGAVIK
                     AVGTKVSLQVSQGKGSQSRRLLIGQRASPEDESRYEIEFVAEPNDTTLSIQVETGGDL
                     SEAIDKVWVVSTP"
     assembly_gap    3060..3159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3160..4472)
                     /gene="tatC"
                     /locus_tag="PLD05_14610"
     CDS             complement(<3160..4472)
                     /gene="tatC"
                     /locus_tag="PLD05_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOP78719.1"
                     /translation="MTFGEHLEELRQCLFRAIWGLAIGVVIGLIFGNRVVALIQTPLE
                     KALTKYYVGQSHREVTKQIQRLSEQGVELPFSAEAAAKFVIDHQVLAEEFLISPRELV
                     TQFEWSLKEMTRRWMADKGSLEQALNVLQKMSASPDSKELEVTDKAVITEAVQILRRH
                     IGPGTLSEASEAFGGLIDLADKLAAGEQLSAEDSHLLRNGLQRMIGQLASAIESVQNT
                     NQALAGLVLATRQEPGSFEQSELMHVFLWRPAKEDPRIRAKSLSAHEAFSIYMKASFL
                     LGAIISSPWVFFQIWSFVAAGLYRHERRFVYIFLPFSLALFLAGATMAFVYVFEPVLT
                     FLFQFNEWLGIQLEPRISEWLSFVLILPLGFGIGFQLPLVMLFLERIGIFTLQDYASQ
                     WRIAVVVIFVISAILTPPDPSSQLLMAIPLCLLYFGGMALCKFFPR"
BASE COUNT         1244 a         1406 c         1422 g         1192 t
ORIGIN      
        1 tagctcgcga aaagcgggcc gaacgcaagg caggtcgccg acctcgcctt gaaacacacc
       61 cggaaattcc gggcaccgtg aaggagggac tacccgaact cgtcgaacca acagcaacag
      121 caaatcaaat ttcctcagag gtcacctcct ccgatttgat ggccacttcg gcctcgcccg
      181 tgatggaggg tctcgcgaac atgttgaatt cacccgaagg ccttgctacg gcgattgtcc
      241 tcaaagagat cctcgatcgg cccgaggctc ggtggcacta aagtcggatg ttccttttgt
      301 cagtttggcc ttcggctaag actaaggttg cctacaacgc tagccagtag taggccatac
      361 caatagaatc cgtcagtcag tttgcattgg aggtaacgat gtcggtgcct attgcgaatc
      421 ctgatagaac gcgtatcggc tggattggaa ccggagtcat gggctccgcg atgtgtggac
      481 acctcctcaa gggggggtat cgatgtacgg tctataatcg cagtcggagc aaggcggaag
      541 cattacttgc cgccggtgcg acctgggccg attctccgaa acgtgttgcc gaggtctcag
      601 acatcgtatt cacgatagtc ggatatccta atgatgttcg cgaggtcatc ttagggccgg
      661 acggggtcct cgcaggattg cccactaacg gcgtcgtcgt cgatatgaca accagtgaac
      721 caagtttggc catagaaatc gctaaagccg ctgcggcgcg gggatgtcat gcgttggacg
      781 cacctgtgtc aggtggtgac gtgggtgcgc gagaggcacg gctttcaatt atgatcggcg
      841 gtgagccgga cattgtcgcg gcggtgatgc catgttttca attgatggga aaaaccatcg
      901 tccaccatgg cgggccgggc gctgggcaac atgccaagat gaccaatcag attcttattg
      961 cttctacgat gattggggta tgtgaaggtt tgctgtatgc ctaccgggcc ggcctggact
     1021 tggaaaaagt catcgagtcc gtggcctccg gagctgcggg cagttggtcg atttctaatc
     1081 tcgggcctcg aatcatccag aaccgttttg agcctggttt ctatgtggaa cacctcatca
     1141 aagacctggg tattgccatc aaagaggcca aacaaatggg gttggtcctg cccggactgg
     1201 cactggccga gcaattgtat ctcgccgtcg cagcatgcgg tcacgcacgg agcggcactc
     1261 atgcccttca aattgcccta gctcggctat cgggaatcga ctggttaaat cgcaagtgac
     1321 ctgacaacaa agagttcact tacggctttc agctttatcg caaaggcgtg ccaatgttaa
     1381 gaaaaatcgt catcgctcag ttcctcttcg tgcttggcct tgcctggccg gaagtccgtg
     1441 cttggggtcc ccatccacag atcacaaaag ctgcgctgga ggttttaccc catatcgagg
     1501 aggtcaaggc gcagcttggg gaagacaatg taatggccct aacgagttac tgctggatgc
     1561 cggacatgcg agggcgcgac ctgggcacat tttacgccga cgactatctg ctgatccgcg
     1621 cgttgccccg ccatattggg catacgatgc cggctgtctc tgcagccttt gagccgtatt
     1681 ttcgtcgagc acttcaggcc ctgcgcaccg aaagtccggc caacgcctgc cgccaacttg
     1741 gaacgctggt ccatttcgtg gaggatgttg gggcgccgcc tcacgccaaa gaaaattgtc
     1801 cccaccacag cgaattagag aattgggttg atgcccagtt gatcacgatt caaggttatc
     1861 aacctcggtt gctcggtcaa agcgacgagg aagccctgca gggtcttcaa gccagaatcg
     1921 ccgaactggt tgcattttcc aaggaacgtg ctgaacgtgc cttgcccatt gtgtcctctc
     1981 cgaatcctga caggtcaaag gtagaagaga ttattttgga gtcagccatt gaatctgcca
     2041 aggtaacagc cgatcttctc ttcacgatgt ttaccctcgg tctgcggccg aattcggggc
     2101 caggccagtc aacgcttcgc ggcacaatca cggccccagc tgcaccggga aatgacgatc
     2161 aatcgccgcg agttgtcctt cttaacactg attactcgac cttagcagaa gccacgagca
     2221 tcggttcctc cggcggctgg cagggcaagt ttgtgtttcg tcagcttcct gccggcaaat
     2281 accaactgtt tgtttatcga atcggggcac aaccggccat cttgcccgta gaacttcgtg
     2341 atggggaggt gacccaccta acgatcgaac tgatacccag cgatccacag ggtaaccttg
     2401 tttacaaccc agacggtaaa acccgggtcc tggtgcccga tttgccggac cgctggtcaa
     2461 tgagtacctc agaaaaggcc aagtcgtggt caagtaccgt cgttccactg aaggcgggcg
     2521 agaaataccg ctgcggagcc gttatcaaag ccgtgggcac aaaggttagt ttacaggtct
     2581 cgcaaggaaa ggggagtcag tcaaggcgtc ttcttatagg gcaaagggct tcgcctgagg
     2641 atgaatctcg atacgaaatc gagtttgtcg cagagcccaa tgatacgacg ctctcgattc
     2701 aggttgaaac gggcggcgat ctgagtgagg ccatcgacaa ggtgtgggtg gtaagcacac
     2761 catagataga taaactttgc aacacggttc gaaaacctcg gagccaatgc cggccagtgg
     2821 cgaggcacct actatacttc ttatctacgt tggatttgca ccagaaagcc acacgcaatt
     2881 ggatttggta ggcctagccg gctgccgcac agggctgaat cttcagggaa actttggcaa
     2941 gattcgcgtc aacgggccac ggtcagatag aaagctgctg ctctctcagc ggttttcttt
     3001 cttgcggggg aaaaacttac atagggccat gccgccaaag tagagcaggc atagtggtan
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gggggaaaaa cttacatagg
     3181 gccatgccgc caaagtagag caggcatagt ggtatggcca tcaggagttg gctagagggg
     3241 tcgggcggcg tcaaaatggc cgagatgaca aagatgacca ccacagcgat ccgccactga
     3301 gaagcataat cttgcaaagt gaagatgcca attcgttcca gaaagagcat aacgagcggc
     3361 aattgaaacc cgattccgaa gcccaatggg aggatcaaga caaatgacaa ccattcgctg
     3421 attcggggtt ccagttggat ccctaaccac tcgttaaatt ggaagaggaa cgtcaagaca
     3481 ggctcaaaca catagacgaa cgccattgtt gccccagcca gaaatagggc cagactgaag
     3541 ggcaggaaga tatagacaaa acgccgctcg tgccggtaga gacctgccgc cacaaatgac
     3601 caaatctgga agaacaccca agggctggag ataatggcac ccagaaggaa ggaagccttc
     3661 atgtagatag aaaaagcctc gtgtgcactc aggcttttcg cccgaatccg tggatcttct
     3721 ttggccggtc gccacaaaaa gacgtgcatg agttcgcttt gctcgaagga accgggttcc
     3781 tgccgagttg ccaacaccaa tcccgctaag gcttggttgg tattttggac ggattcgatg
     3841 gctgacgcca attgcccgat catgcgctgg aggccgtttc tcaacaggtg gctatcttct
     3901 gccgaaagct gttctcccgc tgctaactta tcagctagat cgatcaatcc accaaacgcc
     3961 tcagaggcct cacttaatgt acctggaccg atatgacgcc gaagaatttg caccgcctcg
     4021 gtaatcaccg ccttgtcagt tacttctaac tctttcgagt ccggactcgc tgacatcttc
     4081 tgcagcacat tcagtgcctg ctccaaactg cccttgtctg ccatccatcg tcgcgtcatt
     4141 tccttgagcg accattcgaa ttgagtgaca agctcacgcg gcgaaatcaa gaactcctcg
     4201 gccaatacct gatgatcgat gacaaacttc gctgcggctt ctgcactgaa gggcaactcg
     4261 accccctgtt ctgacaatcg ttgaatctgc ttagtcacct ctcggtgtga ttgtcccacg
     4321 tagtatttag taagggcctt ttccaggggg gtctgaatta acgccacgac ccggttgcca
     4381 aagatcaaac caatcacaac accgattgcc agtccccaaa tagcacgaaa aagacattgg
     4441 cggagctcct ccaagtgctc gccgaatgtc atcgttgatt cacgaaagag atcttcgtcc
     4501 gatttggggg gtggtaatag acgtatcatt gagcacaacg actattcaaa gtgatccgta
     4561 cgacgttgtc gttcgcgggg ctcgcccacg gtgggcgcat ctttgctatc ttatcagcgc
     4621 ctactgacac gggcaatcgc tggcgtccca ggggttcacg cacttttttc gaagccacgg
     4681 cgcacatttc ggataaagct tgcgagcacg gctggcttca aatgtgcgtt ttgcgtattt
     4741 agcgcctatc ccacaacctc ccttctcccg ttcggcgtga gggggccggg gtgagggtgg
     4801 ctcgaatcgc tgctggtttt ctcgatcgac tccgacgatg ccttaccctc accccaaccc
     4861 tctcccaaag ggggagggaa ttttgggata taggctctga gatatatact ttgccgtttt
     4921 gtagtttcgc tgcgacttgc atgtattgtg ccccattttc gtattccaaa tgcacgtggg
     4981 ctctggattc acttccggta ctgcgtggcc caagcattcc tccattacct gccgttttct
     5041 ggcgtgttac agcgtcaaaa gtggctcaac cccagctggc gtacgaacga atagatacca
     5101 cggagcactg tatctccaac atttcggtgt cggcaatcca ccagatatca agcgtttcct
     5161 agaaatcctg caaacaagtc aaccgtattg atttcgcacc cattgcacat gagaatattg
     5221 ctcgaagcga attgtgcacc tgtagggagg ttccagaaga agacaggaga aacccatgga
     5281 aattcatcgg gtagtagcga gtgtttcggt gttaggtctt ttctgtttgt tcccggcgca
     5341 gacggcgcta cccgaagatg gaca
//
LOCUS       DAOSUT010000225         5355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_6886_length_5355_cov_9.172642, whole genome shotgun sequence.
ACCESSION   DAOSUT010000225 DAOSUT010000000
VERSION     DAOSUT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_5355_cov_9.172642"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..968)
                     /locus_tag="PLD05_14615"
     CDS             complement(390..968)
                     /locus_tag="PLD05_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78720.1"
                     /translation="MQSGITLATWFKRESRRVHGLFTETDEEADQRRLIEWLEARGGA
                     ATVRDLTRGPRRFRNKPNEAEAALTALVNAGLGHWESPPSNQKGGVQHVFFALSPSVT
                     VTKTPLKDLKTKVSVTVTVVTPKLHGPRRRKRLLLSPPYVAMMPARLVFPRMEVMMTA
                     IGWRSPYESDRATLSAWRGRGLGGWGTEITHP"
     assembly_gap    1986..2085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2219..>5355
                     /locus_tag="PLD05_14620"
     CDS             2219..>5355
                     /locus_tag="PLD05_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOP78721.1"
                     /translation="MPQIFDNVTLKLIEGLEKAFDKAVAASFCIGYLNLRGWDQVAGL
                     VETLQGGDEDHACRVLVGMYRPPDEEMRALASLRLEQEAIDGQVRARLISRIEQIFRD
                     QLTVGVPSASVEQALRGLAAQLRARKVFVKAFLQYPLHAKLYLVQRDDPVAPLVGFVG
                     SSNLTLAGLSRQGELNVDVVEQDAAKKLQAWFDERWGDELAVDLSDKLATLIETSWAR
                     SKLVRPYLVYLKIAYHLSEEAGEEESRFRAPQIFAEKGTPLLNFQEKAVSLAIHHLHR
                     RGGVLLGDVVGLGKTLMATAIARVLQEDDDSNTLVICPPKLLPMWEQYLQDYEITGKV
                     LSLGKCDELSHLRRYRLVIIDESHNLRNRKGKRYQAIQEYIERNDARVLLLTATPYNK
                     QFTDLSNQLRLFLDENEDLHIRPERFFQQWIKDGKTEADFIAQFGTSPSSLLAFEKSP
                     FPEDWRDLMRLYLVRRTRHFIMKNYAEFDETRQRYFVRLNGEPKYFPVREPRRVSFKI
                     DENDPSDQYARLFRDDVVQIIEQLVLPRYGMAQFLIENADRLANEDQKRILDNLNRAG
                     KRLIGFCRTNLFKRLESSGHSFLLSVNRHILRNLIALHALRNGLPIPIGTQDPAMLDT
                     AINDADDELIIGAENDDPTAVDAREIDAGPVLQTATLAAYQALAAKTYKAYATKFRTR
                     FDWLDPKFFQPELANVLENDAQALFTVLEQAGPWNPDADAKLTALVELLTHHHPKEKV
                     LIFTQFADTARYLHKQLAKRGITDLGVATSEIHDPVSLARRFSPSTNGGLRNGETELR
                     VLVGTDVLAEGLNLQDAHIVVNYDLPWAIIRLIQRAGRVDRIGQKHDTILVYSFLPAD
                     GVDRIINLRTRLFRRLQENQEVIGTDESFFGEDAATKLRDLYTEKAGTLDDDQTDDDI
                     DLVSLALQVWNSASPEDKKAALSLPANVFATRALPETADPSQHPPGVIAYLRFPHRED
                     TLVRVDEHGNLVSQSLSSIFRAAACAPDTPPLPRSESHHDLVARCVEIVTQAQISTFG
                     LLGPLRGVRRKLYERL"
BASE COUNT         1158 a         1519 c         1386 g         1192 t
ORIGIN      
        1 cattaacgaa gacaagatcg agattagtga agtgtggata gtggctctgg ttgtcatttc
       61 tccggaaagt cttggtctaa gcaacggccg cgctccttaa ttccgcaaat gcccccgtga
      121 gaaaacttta cctattttct ccggagcgcg ctgtgttttg ttgcgttgtg gtaatctggg
      181 taaactttgt ccaaggacaa tgtgggggtg acaggcccgg cccataacgc tgcgatgtta
      241 cagcgcgcgt ctacatcagc gtcttcatgc gcgtctacag aagcgccaca aatgaatttc
      301 cctttgggag ggccgggcgg ggtagtcctg ctcaggttgg acaacccttc tccaaaaggt
      361 caggccgggt ggcgttggtc aacgccccct tacggatgcg taatttcagt tccccatcca
      421 cccaatccac ggccacgcca ggccgataaa gtagctcgat cggattcata aggtgatctc
      481 caaccaatcg ccgtcatcat cacttccatc cgtgggaaca ccaatcgcgc cggcatcatt
      541 gccacgtatg gtggggataa caataggcgt ttccgtcgtc gtgggccgtg aagtttcggt
      601 gtcaccactg tcaccgtcac cgaaaccttc gttttcaggt cttttagagg ggttttggtg
      661 acggtgacac ttggagacaa ggcgaaaaac acgtgctgga cgcccccctt ttggtttgac
      721 ggtggcgatt cccaatgccc cagcccggcg ttcacaaggg cggtaagggc cgcttccgcc
      781 tcattaggtt tgtttcgaaa ccgacgtggt ccacgggtaa ggtctcggac cgttgccgct
      841 ccccctcggg cctcaagcca ttcaattaac cgccgctggt cggcctcttc gtccgtctcc
      901 gtgaataaac catggacgcg ccgggattcg cgtttgaacc acgttgccaa tgtaatgccg
      961 gattgcatgc ttgcggcatc cacggcatcc gtggtttttt agattctgcg cgcggtatca
     1021 gcgttaataa aacgacggag tttcgtatcc ggaaggaccc acggcagtgt acaaacaaac
     1081 tgtgcttagt ttcgtgactg gtcccggggg cccctcggag aatttttcgc tacgaatgac
     1141 ccataagcaa aagaaagggg ataagcaccg gcccctccat cgccccccca gtaaaccatg
     1201 ccaaatttgg ttttgctctc ccttgaccgg tcacagctcg ccagaaccat gccatgagag
     1261 agccccttcg ttttgttggc atcccactgg attgcagtcg gtaggccaac gcccctatca
     1321 ctcgattttg tgtggaacaa tagccacaat tgttcccgga actcccgggg ttggtcccgg
     1381 tatcatttct cgcaaggcag ccggatggta tcccttcggc ccgtatctct ctttaaatgg
     1441 gcgccgccat ggcccaagga tttttcccct tgtcgtaggc tatggacaga tcaataggtt
     1501 tgtaattctg ttcaactcgg gagggctcca gcccccccgg tacccgagca ggtatagtag
     1561 ttttcaagat ggttgcctta gatgattcgg ccacccatcc agctagcaac cgcttgagcg
     1621 gttccgatat tggcagcgct ggacggggac ctacattcca gtgcgtgctg gatgggaaac
     1681 tcgtctcgac caacttttta ggcttgtcta ctgccccgat ttgcaaagct gtgagagagg
     1741 aaatgcaatt ccagtcgcca ccaaccgacc caatccttgg gacggcttga agagaagctc
     1801 ttatcggctt tagacgaaaa cgcgggggta aaaaggcaaa actcgggcca accgaaaagc
     1861 tggtggactg gcgtcagccg tagtttgtgc gagctcccct cacacggagg gtcaacacga
     1921 aaaagtcccc aaacgccata ctcgcaatca tttctgcccc cacgttggac agattttgtc
     1981 caaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatgg aaagggaatc
     2101 gctacgacgc tgtgcgggga atttggaaac ctgctccggg ctcttttggc ggtccgaccg
     2161 tggcgagatg gtacgactct accgtaacat tcgtagttaa aaaccgtagg aggacaccgt
     2221 gccacaaatc ttcgacaacg tcacgctaaa gctcatcgag ggattggaga aggcctttga
     2281 caaggccgtg gccgcctcct tctgcatcgg ctatctcaat cttcgcggtt gggatcaggt
     2341 agcaggtctg gtggaaacgt tgcaaggcgg ggacgaggac cacgcctgtc gggtgcttgt
     2401 tggaatgtat cgccccccgg atgaggaaat gagggcgctt gccagtttga ggcttgagca
     2461 agaggcaatt gatggtcagg tacgtgcgcg tctgatatcc cgtatcgaac agatctttag
     2521 ggaccagctc accgttggcg tgccatccgc tagcgtggag caagcccttc gtggcttggc
     2581 ggcgcagctg cgggcccgaa aggtctttgt gaaggcattt ctgcagtatc ctcttcacgc
     2641 caaactatac ctggtccaac gggatgaccc ggtcgcgcca ctcgtcggtt ttgtcggcag
     2701 cagcaaccta accttggccg ggttatcgcg ccagggcgaa ctaaatgtgg atgtcgtcga
     2761 gcaggacgcc gccaagaaat tgcaggcctg gtttgacgaa cgctggggcg atgagctggc
     2821 cgtggatctg agcgacaaac tcgccacact catcgagact agctgggctc gcagtaagtt
     2881 ggtacgcccc tatttggtct atcttaaaat cgcatatcac ctctccgagg aagccggaga
     2941 ggaagaatct cgattccgtg caccgcagat atttgcggaa aaaggcacac cgttactcaa
     3001 ttttcaggaa aaagccgtct ccctggctat ccatcatctc catcgccgag gtggagtgct
     3061 gttgggcgac gtggtcggtc taggcaaaac cctcatggcc acggctatcg cccgggtcct
     3121 ccaagaggat gacgatagca acaccttagt catttgtcct cccaagctct tgcctatgtg
     3181 ggagcagtat cttcaggact atgagatcac tggaaaggtc ctcagtttgg gcaagtgcga
     3241 cgaactttct catctgcggc gctatcgctt agtgatcatc gatgaaagcc acaacttgcg
     3301 caaccgtaag ggtaagcgct accaggcgat acaagaatac atcgagcgga atgatgcccg
     3361 tgtccttttg ctcactgcga ccccttacaa caagcaattt actgacttaa gtaatcaatt
     3421 aagactcttt ttggacgaaa acgaggacct ccacatccgc cccgaacggt ttttccaaca
     3481 atggatcaag gacggcaaaa ctgaggccga tttcatcgct caatttggca cttccccatc
     3541 ttccttgctt gccttcgaga aaagcccatt ccccgaggac tggcgagatc tcatgcggct
     3601 ctacctggtt cgccgcaccc gccactttat catgaaaaat tacgccgagt tcgatgagac
     3661 acgtcaacgt tactttgtgc gtctcaacgg cgagcccaag tattttcctg ttcgagagcc
     3721 caggcgggtc tcgtttaaga tcgatgagaa cgacccttcg gatcagtacg cccggttatt
     3781 tcgggacgac gtcgtgcaga tcatcgagca actggtgctg ccccgctatg ggatggctca
     3841 gttcctcatc gagaacgccg atcgcttggc caacgaggac caaaaacgca tcctagacaa
     3901 tctcaatcga gcgggaaagc ggttgatcgg attttgtcgc actaatttgt tcaagcgact
     3961 ggaatccagt ggccatagtt ttctgctctc agtcaaccgg catatcttgc gcaatctaat
     4021 cgcattgcac gcgctccgga acgggcttcc cattccgatc ggcacgcaag accctgccat
     4081 gctggatacc gccatcaatg acgccgacga cgagctcatc atcggcgctg aaaacgatga
     4141 cccgactgct gtcgatgccc gcgagatcga cgccgggccc gttttgcaga cggccaccct
     4201 ggctgcatac caggctctgg ccgcaaagac gtacaaggcc tacgccacca aatttcgtac
     4261 ccgtttcgac tggctggatc ccaagttctt tcagccagag ttagccaacg tcctggaaaa
     4321 cgacgcccaa gccctgttta ccgtcctgga gcaagccgga ccgtggaacc ccgatgccga
     4381 tgccaaattg accgcccttg tggaactcct tacccaccac caccccaagg aaaaggtctt
     4441 gatcttcacc cagtttgccg acacggcgag gtatttacat aagcaactcg caaagcgagg
     4501 cataaccgac cttggtgtgg cgaccagtga gattcacgat cccgtttccc tggcacgccg
     4561 tttcagcccc tccaccaacg gcggactgcg aaatggagag accgagttgc gtgtcctggt
     4621 tggcacggat gtgttggccg aaggcctgaa tctgcaagac gcccacattg ttgtcaatta
     4681 cgatttgccc tgggcgatca tccggctaat tcagcgagct ggccgtgtgg accgtatcgg
     4741 ccagaagcac gacaccatcc tggtctattc ttttcttcca gccgatggcg tcgatcgaat
     4801 catcaatttg cgaacgcggc tgtttcgccg cttgcaggag aatcaagagg tcatcggcac
     4861 cgatgaatca tttttcggcg aagatgctgc caccaagctc cgcgatcttt acacggaaaa
     4921 ggccggtacc ctcgatgacg accaaacaga tgacgacatc gatctcgtca gtcttgccct
     4981 ccaagtctgg aacagtgcct cgcccgaaga caagaaggcc gccctatcgc tgccggcgaa
     5041 cgtctttgcc acgcgagctc tgcccgagac tgccgatccc agccaacatc cacctggtgt
     5101 gatcgcctac ctgcgttttc cccatcgcga ggataccttg gtgcgtgtgg atgagcacgg
     5161 caacctggtt tcccagtctc tctccagcat ctttcgggcc gccgcctgcg cccccgatac
     5221 tccgcctcta ccccggagtg aaagtcatca cgaccttgtg gcccgttgtg tcgagatcgt
     5281 aacccaggct cagatatcta ccttcggtct cctgggtcca ttgcgtggcg tccgtaggaa
     5341 actctacgaa cggct
//
LOCUS       DAOSUT010000226         5238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7044_length_5238_cov_8.862628, whole genome shotgun sequence.
ACCESSION   DAOSUT010000226 DAOSUT010000000
VERSION     DAOSUT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_5238_cov_8.862628"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1682
                     /locus_tag="PLD05_14625"
     CDS             363..1682
                     /locus_tag="PLD05_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78722.1"
                     /translation="MLAPTVDPAPKPEGQSQAVPGGSTMDQASSPGDSAVSKPAGAAS
                     LPDDGWVTKGSRSQLARPGGGQASAATSSIKPPPASLPAPQVLPQTPGPTYGGGIQAT
                     GIPYPYGTPAPVPQYLGRSWPGQTGLAPRPILGGKASQSHGFLASVGRWWNSLWGKKP
                     LEQPAGGSFNSSTNSLSSWNGAGTSHAGNVVPWSGSAVGPPASGTSGGSTSPQVMQPY
                     ATPYALPPNSSQGSALSSPPLTAGPAQSGTGGQAHTTLMPPHSTTEGTAKLVTPGTSA
                     GSSGAPGTLGGNVYGGWTATPQAASVAPSSPQMRSTLPPYPHYGSGFGTALPFPRASE
                     PPAALWNGGAILPRKQTDGRSLMDRLRAWWADVTAPKGIQGRQALLPPSPSDGRILGV
                     IPRRNSVPTTDPFSSYGGGTVSPGTPQAFGTGMSYQPPAAVTPPVLP"
     gene            complement(1735..3279)
                     /locus_tag="PLD05_14630"
     CDS             complement(1735..3279)
                     /locus_tag="PLD05_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78723.1"
                     /translation="MIAARIRLVGTLFQLLKVSIAALACWVMTLFSSVFAYTPPNIIL
                     CVGDDHGWEETGYNGHPYLKTPILDSMAATGLRLDRFYAAAPVCSPTRGSIITGRHPN
                     RYGTFSANWSIRPEEIGVAQILARAGYACAHFGKWHLGPVKAESPTNPGAMGFERWLS
                     HDNFFEVNPFLSRNGGPPERFEGESSEILVDEAIRFLREAKDNGRRFFVAIWFGSPHE
                     PYVGLPSDLALYDNLPQEYEERTVRLTDVKTGLATKRPLRDVLRERYAEITAMDRAIG
                     KLRQFLEKEGLRENTLLWYLSDNGTPPEAAVMVPFRGMKGSIYDGGLRVPSVMEWPAV
                     ISRPRASDVNCVTSDILPTLCDLTGQPLPNRPLDGVSLKPVIFGEKWERPSPICFWQY
                     DIGHETRNNPQPYIDPKLQEGTTPLAKQMGGRWTRNFQNLRHLSITEEDFRGPRAILE
                     NQYKLVIIDRAGGTQSKELFDVREDPAEKNNLIDMKPDIARQLEQKLRTWQESVLKSL
                     LGEDYK"
     gene            3497..4408
                     /locus_tag="PLD05_14635"
     CDS             3497..4408
                     /locus_tag="PLD05_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOP78724.1"
                     /translation="MKCGVLFRILSTFLAAYGILGMNPQSADGQTGVILVAHGSPSPH
                     WNQAVAEVVERVREHLKDEPGIAGVRLGYLEKARPTIADAVSELTALGCRRIVAVPMF
                     IAASGHVQYDVPTVLGVLHDPKVARTLREEGITLVCPTVPITLTPPLSEGGCLDAYTL
                     SEVKRLSQNPQREVLLLLLHGDDSFRPILDNLAKRVISSVQKEVPLSAGDWCYVGVGQ
                     HYGSQAVPKIKEAMRRGQRVLVVAIFLASSARHFHERWVQQEANGNDPFQGREVAFSD
                     QPLTQHPALVDDVIQFVREAIRRNCGE"
BASE COUNT         1072 a         1525 c         1553 g         1088 t
ORIGIN      
        1 aacgaccctg ggcaagtcgc gaagggagtc gccggctcgc tcatctcatt tgaggcggcg
       61 atagaccaat gcgttggcga tgcaaccatc ttggtaagag tccatgccaa aaagcccccc
      121 tctcccttcg ggagagggtt ggggtgaggg caaacgaacg tcggagtcga tcgggaaaac
      181 cggcaggggt tcgtgccacc ctcacccccg acccctctcc cgccaaacgg gagagaggag
      241 attttgggat acgttctaag tttgttttcg ctctcacagc gataataggt gtccttttgc
      301 cgggggggcc agtatgggcg aattcagaga caggtcctga actgttcgct ccggatattg
      361 tcttgttagc ccccactgtg gatccggccc cgaaaccgga aggtcagtcc caagcggttc
      421 ccggcggttc aacgatggac caggcgagct ccccgggcga tagcgccgta tcgaagcctg
      481 ccggcgctgc ctcgcttccc gacgatggtt gggtcaccaa aggaagtcgt tcccaattgg
      541 ctcggcctgg cggaggacag gccagtgctg ccacgtcgtc gatcaaaccg ccgccggcca
      601 gtctccccgc gccgcaggtg ttgccacaaa cacccgggcc gacctacggg ggcgggatcc
      661 aagcgacagg aataccctac ccctacggta ccccagcccc tgtgccgcag tacctcggtc
      721 gatcttggcc cgggcaaacc ggcttggcgc cgcggccgat cttgggtgga aaggcgtccc
      781 agtctcacgg ctttttggcc tctgtggggc gatggtggaa ttcactgtgg ggaaaaaagc
      841 cgctcgagca accggctgga ggctcgttca attcgagtac taactctttg agtagctgga
      901 atggggcggg aacgtcccac gcgggaaatg tcgtcccttg gagcggatcc gctgtagggc
      961 cccctgctag cggaacatcg ggtgggagca cctcacccca ggtgatgcaa ccctacgcca
     1021 cgccctatgc cctgccacct aactcgtcac agggatcggc cctttcgtcg cccccgctaa
     1081 ccgccgggcc ggcacaaagc gggaccggtg gccaggcgca cactaccctt atgcctccgc
     1141 attcaacgac ggagggcacg gccaaactgg taacaccggg gacctcagcg ggttccagcg
     1201 gtgccccggg aactttaggg ggaaatgtct atggcggttg gacggcgacg ccgcaagccg
     1261 ccagtgtcgc tccaagttct ccgcagatgc gttccacgct acctccatat ccccattacg
     1321 gcagtggatt tggaacggcc ctgcctttcc ccagggcatc ggaaccgccg gccgcactgt
     1381 ggaatggtgg cgcgattttg ccccgaaagc aaacggacgg gcggtcactg atggatcggc
     1441 ttcgcgcatg gtgggccgac gtgacagccc ccaaaggcat ccagggacgg caggccctgc
     1501 tcccgcccag tccctctgac ggcagaatct tgggcgttat cccgcggcga aactcagttc
     1561 caaccaccga cccctttagc agttacggtg ggggaacagt gagtcctggt accccacagg
     1621 cgtttggtac gggcatgtcc tatcagccac ccgccgctgt gacgcctcct gtgttaccat
     1681 gactgtggcg tgggctgggg tgagggcact cttgagtttc gcttttatgc cccctcattt
     1741 gtaatcctct cccaaaagac tcttgaggac cgactcctgc cacgttctca gtttttgttc
     1801 caactggcgg gcaatatcgg gcttcatatc gatgagattg tttttctcgg ccgggtcctc
     1861 gcgtacgtca aacaattctt ttgattgtgt tccgccggcc cgatcaataa tgaccagctt
     1921 atattgattt tccaaaattg ccctcggacc gcgaaaatct tcctccgtga tggaaagatg
     1981 ccgcaaattc tggaagttac gggtccaccg accacccatt tgtttcgcaa gcggagtagt
     2041 gccctcctga agtttggggt cgatataagg ctggggattg ttacgtgtct catggccgat
     2101 atcgtactgc caaaagcaaa ttggcgaagg ccgttcccac ttctccccga aaatcactgg
     2161 ttttaagctg actccatcca gcgggcgatt gggcagtggt tgtccggtta aatcgcaaag
     2221 agttggcaaa atatcgctcg tcacgcagtt gacatctgat gctcgcggcc gggaaataac
     2281 ggcaggccac tccatgacgc ttggcacacg cagtccgccg tcataaatcg aacccttcat
     2341 accacggaaa ggcaccatca cggcggcttc cggtggcgtt ccattgtcgc tgagatacca
     2401 gagcagagtg ttttctcgca gcccctcttt ctcgagaaac tgccgcaact ttccgattgc
     2461 ccgatccatg gcggtgatct cggcataacg ttcgcgaagc acgtcgcgca gagggcgctt
     2521 ggtcgccaga ccagttttca cgtccgtcaa gcgaacggtg cgctcttcgt actcttgcgg
     2581 caggttgtcg tagagagcga ggtcgcttgg taatccaaca taaggctcgt ggggcgaacc
     2641 aaaccaaatg gcgacgaaga aacgtcggcc attgtccttt gcctccctta ggaagcgaat
     2701 ggcttcatcc accaggattt cggagctttc gccttcgaaa cgctccggtg gtcccccatt
     2761 gcgcgaaaga aaaggattca cttcaaagaa attgtcgtgg gaaagccatc gttcaaaccc
     2821 catcgcaccg ggattcgtgg gcgactcggc cttgacaggg cctaaatgcc atttgccaaa
     2881 gtgtgcacag gcataacctg ctcgggcgag aatctgggcc acaccaattt cctcaggccg
     2941 aatggaccag ttggcactga acgtcccgta tcgattgggg tggcgtccgg tgatgatgct
     3001 ccctcgagtt ggcgaacaaa cgggcgcggc tgcatagaag cggtccaagc gaagccctgt
     3061 cgccgccatg ctatccagga taggcgtctt gaggtacggg tggccattgt agcccgtctc
     3121 ctcccagcca tgatcgtcac ccacacaaag gatgatatta gggggagtgt aggcaaatac
     3181 ggaagagaac agcgtcatca cccaacatgc gagggccgcg attgatacct tcagaagttg
     3241 aaagagcgtg ccaactaacc taatgcgagc tgcgatcatc gtacaccgcc tgtcttgcga
     3301 acgccaaaga catcttggaa tgggtcccgc ccacggattg taaaccagat tatgacccaa
     3361 cccgcaagtg tcgccaaggc gtgcgccaac tgtttcggtg aaacagcatg agaactatgg
     3421 attgtcaagt ccaccgcctg aaaggcaact ctcgatgaag gggtttgctt ctcttttgat
     3481 gaaaggagtg cgtaagatga agtgtggtgt gttgtttaga attctgagta cgttcttggc
     3541 agcatatgga atactcggca tgaatccaca atccgcggat ggccaaactg gtgtcatcct
     3601 tgttgcccat ggctccccca gcccacactg gaatcaggct gtggctgagg ttgtcgagcg
     3661 ggtccgggaa catctgaaag acgagccggg catcgccgga gttcgtctag gttacctgga
     3721 gaaggcccga ccgacgatcg ccgacgccgt ttctgaattg acagcgttgg gatgccgccg
     3781 gattgtggcc gtgccgatgt ttatcgcggc atccggccac gtgcagtatg atgtgcctac
     3841 cgtgctgggt gttctccacg accccaaggt ggcgcggacg ttgcgggaag aaggcatcac
     3901 gctggtttgt cccaccgttc ccatcacgct gacgccgccc ctcagcgagg gtggttgcct
     3961 cgatgcctat acgctgtctg aggtaaagcg gctttcgcaa aatccacagc gggaagtgct
     4021 cctccttttg ctccacggag acgacagttt tcgaccgatc ttggacaacc tggcgaaacg
     4081 ggtgattagt tctgtgcaaa aagaggtgcc actttccgcg ggcgactggt gctatgtggg
     4141 cgtcggccag cattacggtt ctcaggcggt gccgaagatc aaggaagcca tgagaagagg
     4201 ccagcgggtc ttggttgtgg cgattttcct ggccagctcg gcgaggcatt tccacgagcg
     4261 atgggtccag caagaggcca atggcaacga ccccttccaa ggccgagagg tggccttttc
     4321 tgaccagccc ttaactcaac atcccgccct ggtggacgac gtgatccagt ttgtgcgtga
     4381 ggcgatccgt cgcaattgcg gcgagtgacg cacgccccgg cgctggtagg ggcgccgtac
     4441 gtattgccca atcggtaagg acggcgtgca tattgtgcga tcgggacgga cggcgtcccc
     4501 gccgtccggg tgcaccggcg ttgcggacaa acggggcaac cacggggggt tgcccctacg
     4561 tctgacgcgc ccattgaaac ccgtaggggc aggcccccgt gcctgcccta cgccttgtgc
     4621 gtggcacggg catcttgccc gtgccgttgc ctgttccgct tttgaccgct tgtctacaac
     4681 gggcgtgcat gttgcccggg catttggtcg ccggggagtc ccggcaaatt ggctcatcgg
     4741 ggacgatgac cctcccgggg cggaatcggg agggacggcg tccccgccgt cccgttccat
     4801 aaaggcggtc tccctggcgt gtgggtgttg caactacggg gctggccgcg tatgtaaagg
     4861 cgtatcgtaa aaaactcccc ctctccctct gggagagggt tggggtgagg gcaagcgaac
     4921 ctcataatcg attgagaaaa ccagcagcga ttcgacccac cctcaccccc gacccctctc
     4981 ccgccaagcg ggcgagggga gactaagctt caagaggcga caagatttat tcgtggcgcg
     5041 gtcttgcaac gtcaggggca cggtggtatt atgtggggca aagagcgatg aaaaactcgc
     5101 aaaaaactga cgattttggg ccgctcaaag gccgtttgag gcgagttttt tgcaggtatt
     5161 tttgccgtaa gattaccatc agaaaagact tacggcacat atcctgtggt tccgggcgaa
     5221 taggagccca ttgaaacc
//
LOCUS       DAOSUT010000227         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7076_length_5210_cov_8.318332, whole genome shotgun sequence.
ACCESSION   DAOSUT010000227 DAOSUT010000000
VERSION     DAOSUT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7076_length_5210_cov_8.318332"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLD05_14640"
     CDS             <1..255
                     /locus_tag="PLD05_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78725.1"
                     /translation="VVPIIIGNSLHCMFLSQALFKRGINVQPIVYPAVEERAARLRFF
                     LTSRHTEEQIRYTIDVMTEEVDKLDPNYRAARLGSMAGHS"
     gene            434..1270
                     /locus_tag="PLD05_14645"
     CDS             434..1270
                     /locus_tag="PLD05_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP78726.1"
                     /translation="MACLSVSETTTFRWSFEEDIHRYRAAGIPAIGVWRQKLSDFGEE
                     KGAELIVEADLRVSHLFWAGGFTGSDGRSYKAAVDDAREAIQTAAELKTRCLIVYSGS
                     RGTHTFNHARRLFRDALKELTPLAQEFDIYLGVEPMHPGCGCEFTFVSDIEEFLRLVD
                     AVGSDRVKLVFDTYHLGLTPDIVAQIPRLVPHIVLVQLGDAKRPPQGEVNRCLLGEGV
                     IPVKAIVDALLDNGYDGFFDVELIGEDVEHCDYTQLLEHARKAYEQLVGPIGPLAVSQ
                     ES"
     gene            1977..2050
                     /locus_tag="PLD05_14650"
     tRNA            1977..2050
                     /locus_tag="PLD05_14650"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2011..2013,aa:Val,seq:tac)
     gene            2408..3898
                     /locus_tag="PLD05_14655"
     CDS             2408..3898
                     /locus_tag="PLD05_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78727.1"
                     /translation="MCAGELLRTWLFVLVGVLLFPLSSFSSAHSPSLNVIVIMTDNQG
                     AWSLGCYGNPDIKTPHIDRLANEGVLFTRCFANNAVCSPTRASFLTGLMPCQHGVHRY
                     LSAGGIQIGPEAYNTLAEFDTLPSLLAQDGYVCGLVGKWHLGDNLSPQEGFSYWVTMP
                     HGHTAGFYNQQIIEGGKIQRYAGYLTELWTQRAIEFIETQKDRRFFLFLAYNGPYGLG
                     GVLDEPIQNRHRRTYENAEMKSFPRLPPHPWNHNYANRVGDLSAIRKYAAEISAIDDG
                     VGEIMASLERCKLKHNTLVIFTADQGLAAGHSGFWGMGDHTRPLTAFDWTMHIPLIFW
                     CPDHIPSGRQVDLLVSNIDVLPTLVEYLQLTWRKTDARKLPGRSFAALLRGEEIPSWE
                     NVVFYEFENVRAIRTDRWKYIERFRQTPNELYDLEHDPQELTNLVDDSRCEAVKRELS
                     QRLYQFFDKYADPKWDLWRGGTSKSGLLMHDLFDNPGPAPSPKAKR"
     assembly_gap    4040..4139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4376..5137)
                     /gene="bioD"
                     /locus_tag="PLD05_14660"
     CDS             complement(4376..5137)
                     /gene="bioD"
                     /locus_tag="PLD05_14660"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOP78728.1"
                     /translation="MSLRDDTERPTGMSKKKPLGLFITGTDTGVGKTYVGALIARNLA
                     QKGLRVGVYKPVASGCQRVKGELVSEDAVTLWEAAGKPLTLGHVCPQCFEAPLAPPAA
                     ARAEGKSVDTRLLREGLRPWLEWAEVILIEGAGGLMSPVSDEDYAADLAYEFGYPLLV
                     VSNNVLGTINHTLLTLIAATTFREGIDVAGILLNEPAPPDPLDASVTSNLQELSARCV
                     PPVLGYVAHGARESEPPVDWLTVARRPTPPRTTSW"
BASE COUNT         1142 a         1376 c         1428 g         1164 t
ORIGIN      
        1 gtcgtcccga tcatcatcgg caactcgctc cactgtatgt tcctttcgca ggccttgttc
       61 aaacgaggga tcaacgtaca accgattgtg tatccggccg tcgaagaacg ggccgcccga
      121 ttgaggttct tcctcacctc acgccatacc gaagaacaaa tccgctacac aattgacgtg
      181 atgactgaag aagtggacaa actcgatccc aactaccggg ccgcccgttt gggcagtatg
      241 gccggacatt cgtagtcgca ctcgatatag tcatccgtgg tgacgcggcc acaatccttg
      301 ggggatcggg cagaaaatat tgccgacccc tttatttttc ttcaggattt ggccatactg
      361 gaaacgcttt gataaggcgg ttctcggacg gttggaaaat ggtcacggtt ggtttagttg
      421 gaggcaaaca cggatggcct gtttgtcggt ctccgagacg accacgtttc gttggtcctt
      481 tgaggaagat attcaccgat atcgtgcggc gggcattccc gccatcggtg tttggcggca
      541 aaaactctcg gattttggcg aagagaaggg ggcggaactg atcgtcgagg cagaccttag
      601 agtctcccat ctcttctggg caggtggttt tacgggcagc gatggccgct cgtacaaggc
      661 agccgtggat gacgcccgcg aagccatcca aacggccgca gaactgaaaa cccgttgtct
      721 gatcgtctat agcggttcgc gtgggacgca taccttcaac catgcccgac gactctttcg
      781 cgacgccctt aaagagctga cgccgctagc ccaggaattt gacatctacc tcggcgtgga
      841 accgatgcat cccggctgcg ggtgcgaatt cacgttcgtc tccgacatcg aggaatttct
      901 tcgcttagtc gatgcggtgg gctccgatcg agtgaagctt gtgttcgata cctatcacct
      961 cggtctgacg ccggacatcg tggcccaaat tcctcggttg gtgccacaca tcgttttggt
     1021 gcagctaggc gatgccaaac ggccgccgca aggtgaggtg aatcgttgcc tcctgggtga
     1081 aggagtgatc cccgtcaaag ccattgtcga tgccctgttg gacaacggtt acgatggctt
     1141 tttcgatgta gaactgatcg gtgaagatgt agaacactgc gattatacgc aactgcttga
     1201 gcacgccaga aaggcctacg aacaacttgt aggccctatc ggcccattgg ctgtctccca
     1261 agagtcgtaa ccaacgcgcc aaaagagagt tgcggccggt aggcgcaggg acctacccca
     1321 ttgaggtggc gcggatcaaa aatatggggc cccatttgtg atatgggggt gctgctctat
     1381 cgactatgcg tgttgcaaag cctgtggcag caaccccaaa cgttgtcttc tcttgggaac
     1441 gaatggatgc attatcctcc tgaattcgga ggtgcgattt atcgcctttt ttagcaacta
     1501 gctctctttc taatttgggg ggaccggcga catcctatgc gactgaagag cctatcccga
     1561 aaagtccccc tctccctctg ggagagggtt ggggtgaggg caagcgaacg tcggaatcga
     1621 ttcagaaaac ccgcagcgat tcgagccacc ctccccccga cccctctccc gccgaacagg
     1681 cgaggggacc ttacaggata ggctctaagt accccggtcg taacgccaac cactcgcctc
     1741 aaacgggaac accgcaaaag gtcttcacgc cgccgattga cgttttccca ttgaaggcgg
     1801 acaataaaat acaaacgtgt cgaagagagt ctgacgaagg aatatgccga gcaccgagaa
     1861 aggaaatcac attccggatg tgagcgccgt caactgttaa cttcggatcg tcctttcggg
     1921 gttgtgtcct gaagtgatac gaaatggaga acgggtgaca aggacgtcgc acattagggc
     1981 gattagctca gttggttaga gcgccaccct tacaaggtgg aagtcggccg ttcgagtcgg
     2041 ccatcgccca tcagggtatt tgcactagtc ggcgatatag cgcaaaacgc ggcaaaaacg
     2101 gggttttcgt gattcggctt ctcatcggtg cagccgattt ctggcgtgtg cggggccgct
     2161 agtggggcca agcccgacaa tacaacggga agagatgttc atgtccgact cccgcattcg
     2221 caggtttact ggatagcgca gatgcaatgc cagacaccgt ttggcaacta gcactacgcc
     2281 atcttcgacc gccattcacg agggttactg gttcggcctc ctctcttgcc tctcttgccc
     2341 gcgttcatca aaatcaccgg cgttgcggat tggttggccg atttcggggg gatttggagg
     2401 tcgggttatg tgcgcggggg agcttctgcg aacgtggctt tttgtactcg tgggagtttt
     2461 actcttcccg ctttcatcct ttagttctgc ccactcgcca tcccttaacg tgatcgtcat
     2521 catgacggac aatcaggggg cctggtcatt gggttgctac ggtaatccag acataaaaac
     2581 gccgcacatc gatcgcttgg ctaatgaggg agttttgttt acgaggtgtt tcgcgaataa
     2641 cgctgtgtgt tcgccgacac gtgcgagctt tctgacgggg cttatgcctt gtcagcatgg
     2701 cgtgcaccgt tacctcagcg cagggggaat tcagatcggc ccagaagctt acaacactct
     2761 tgccgagttc gacacgcttc cctccttgct ggcccaagac ggatacgttt gtgggctcgt
     2821 gggaaagtgg cacctgggcg acaatctgtc tccccaagaa ggcttctctt attgggtgac
     2881 tatgccccat ggtcataccg ccgggtttta taaccagcag attatcgagg gcggaaagat
     2941 tcaacgatac gccggatatt tgacggaatt gtggacccaa cgtgcgatcg agttcatcga
     3001 aacccaaaaa gatcgacggt tttttctctt cctggcctac aatggcccct atggcctggg
     3061 cggggtacta gatgaaccca tccaaaaccg acaccgacgc acctacgaga atgcggaaat
     3121 gaagtctttt ccgaggctgc cccctcatcc ttggaatcac aactatgcaa accgcgtcgg
     3181 agacctctcg gcgatccgta aatatgcggc tgaaatcagc gccattgatg atggcgtggg
     3241 cgagattatg gcatctctgg aaaggtgtaa attgaagcat aacaccttgg tcatttttac
     3301 ggctgatcag gggttagcgg cgggacatag tggattctgg ggcatgggtg accacacccg
     3361 gccgttgact gcgttcgatt ggacaatgca tatacctctc atcttctggt gtccggatca
     3421 tattccaagt ggaagacaag tggacctgct tgtaagtaac attgatgtgc tgcccacttt
     3481 ggttgagtac ctgcaattga cctggagaaa aaccgatgca aggaaactcc cgggcaggag
     3541 cttcgcagcc ctactccggg gagaggagat cccatcctgg gaaaatgtgg tcttttatga
     3601 atttgagaac gtgagggcta tccgtaccga tcgttggaag tacatcgaac gatttcggca
     3661 gacgccaaac gagctttacg acttggaaca tgatcctcaa gagttgacaa acttggtgga
     3721 cgattcgcga tgcgaggcag tgaagcgaga attgtcgcag cggttgtatc agttttttga
     3781 caaatatgcg gatcccaaat gggatctttg gagaggagga acatcaaagt ctggactcct
     3841 gatgcacgat ttgttcgata atcccggccc ggcaccgtcg ccaaaggcaa aacggtgatg
     3901 aaagcaagca cccgaacaca gcgctataaa gcaggccact cacgcttgtc gcgacaataa
     3961 tcgacgacca cagcggttgt catatgggtt tggtagtgcg cgaaaaacgg ctcgtagggg
     4021 caaccccctg tggttgcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     4141 taggggcaac cccctgtggt tgccccgttt ggcacgatcc cgtggtcggc ctcatttggg
     4201 gcaattcgca ctgttgtcca cgccgatgcg gcccttgacg tttccagaca ccacaagaaa
     4261 tactcgtatc ccgctttgcc aaaacggtgt ccaagccggc gcgggtttga cagggcaata
     4321 acggttgtgc ttagggatat cgtttcacgc atgcatgctt ggggggccgt ctaactcacc
     4381 acgaggtcgt tcttggcggg gtcgggcgtc gggctacggt gagccaatcc accggtggtt
     4441 cactttctcg ggcaccgtgg gccacatacc caaggacagg cgggacgcag cgggcggaga
     4501 gttcctgcaa gttgctcgtg acgctggcat cgagaggatc gggcggggca ggctcgttta
     4561 gtaagattcc cgccacgtcg attccctccc gaaatgtcgt tgcggcgatg agagtcaaga
     4621 gtgtgtgatt gatcgtaccc aggacgttgt tactgactac caacaaagga tatccgaact
     4681 cgtaggccaa atcggcggca taatcctcat cactgacagg ggacatgagc ccacctgcac
     4741 cttcaataag gatgacctca gcccattcca gccaaggccg cagaccttcc cgaaggagcc
     4801 gcgtgtccac agatttgccc tccgcccgag cagcggccgg tggggccagg ggggcctcga
     4861 aacattgcgg acagacatgc cccaaagtga gcggcttgcc agcggcttcc caaagcgtga
     4921 ccgcgtcctc tgaaacaagt tctcctttta ctcgctggca accacttgcc accggcttat
     4981 aaaccccaac acgtaagccc ttttgcgcca aattgcgagc aatcagggca cccacatagg
     5041 tcttgcctac gcccgtatcc gttcccgtta tgaaaagtcc caggggcttc tttttcgaca
     5101 tgccagtagg cctttccgtg tcatctcgta agctcatgat gattatgatt ctttcgagcc
     5161 tatcccaaaa agtccccctc tccctccggg agagggttgg ggtgagggca
//
LOCUS       DAOSUT010000228         5151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7176_length_5151_cov_10.358124, whole genome shotgun sequence.
ACCESSION   DAOSUT010000228 DAOSUT010000000
VERSION     DAOSUT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_5151_cov_10.358124"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..684
                     /gene="trxA"
                     /locus_tag="PLD05_14665"
     CDS             358..684
                     /gene="trxA"
                     /locus_tag="PLD05_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOP78729.1"
                     /translation="MGKAISLTEQSFDQEVLQSDVPVLVDFWAPWCGPCRMIAPTIEQ
                     LAEEFAGQAKVCKLNVDESTAIAARYSVTSIPTIIIFKNGQVVDRYVGAQSKERLRDA
                     LVEATR"
     gene            complement(773..2092)
                     /locus_tag="PLD05_14670"
     CDS             complement(773..2092)
                     /locus_tag="PLD05_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78730.1"
                     /translation="MKAKATRRQFLGVTGVVGGVLSGVPMLIPASALGLTAEPAPSER
                     ITLGVIGIGPRATYDLQAMLKLPDVRCLAIADVQASRREAGKKLVDEFYGNQDCVTYR
                     DFRELLDRKDIDTVLVATGDRWHAPASIMAAEAGKDVYSEKPCGITIAKCQELAKTFS
                     RTKRIFQAGTQRRSVPNFIYAVELARSGKLGKIHTLYASVYEPQLDNSWLPREQTPPR
                     DVVDWNLWLGPAPWRPYNQKYVQGGWRGYFDFDSGARLLDWGAHTVDLCQWAIGADNT
                     VPVEYEPMGDKIVCTYASGVKLILEFLKTPFGDRSPKFITELGTCPVRFVGEEGWVET
                     GDNGGIEVHPDTLKKEVPEAITRVRGLDVSAHARNFFDCVKSRAKTVTNEDVMRHSHV
                     ACHAAAIAWILDRKLRFDPKTEKFIGDEEANLLCARPERNPFTEHGV"
     gene            complement(2468..3727)
                     /locus_tag="PLD05_14675"
     CDS             complement(2468..3727)
                     /locus_tag="PLD05_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78731.1"
                     /translation="MFKDMIARLLWACFMSFMLVGDRIAWATDYVTRATGSPSAYWSS
                     RVLGRPTSTGKVIDSRPIVTSQVLQPDRATEYSIQTPRTRFISENIQPVPEMIPPGEP
                     ITPVEGVEDGEYLDSAFVPVEDNLLSCGSGYCEGACYGCFPFGPWIQHLSVFAGAHGF
                     KGTADLGRNGNFGLHEGLNYSAPLGGPWNIGYQVGVQVVHSDFAGHQTLTWYGQDQIT
                     NASRAQMFFTAGLFRRPECDRWQWSVLFDWMHDDYYVDSDLLQIRHEISFFLDDQREI
                     GYMGMYGVKDDLLLLRDGNEQERRYLDLSVLDRYAMFYRWHFCRGGEGRFWAGVSGYG
                     DGLIGGDIVLPLDRSLALEANFAYLAPNTGTGADGQMDEHWSLSIGLVWYPGRPVDEA
                     LGDLFRSVQGVADNTSMMFHGRIRNSP"
     gene            complement(3965..5119)
                     /locus_tag="PLD05_14680"
     CDS             complement(3965..5119)
                     /locus_tag="PLD05_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOP78732.1"
                     /translation="MKFRTKAIHLTPPGRSTGALVPAIYLSTTFAQPAPGQWGEFDYS
                     RSGNPTRKCFEDAIAELESGVGGLAFSSGMAAIHCVTMLLECGDHIVTGYDVYGGTYR
                     LLHKIINRSGITVTMVPTNDLDAVEAAFTPRTRLLWIETPGNPLLSITDIAACAEIAH
                     RHNAILGVDNTFATPVLTRPLELGADIVMHSATKYIGGHTDVLGGVLVVRDSELFKRL
                     YFIQNATGAVMAPLEAYLCHRGLKTLDLRVREQSRTAHQIADFFHNHPRIRKVYYPGL
                     ADHPGHEIAARQMSGGFGAIVSLELDGDAGLVQRFAKSTQIFQLAVSLGAVESLIEQP
                     ATMSHASYDAAFRAKHGIRDELIRLSIGLEDPEDLIADLEQALDRATKGG"
BASE COUNT         1188 a         1444 c         1421 g         1098 t
ORIGIN      
        1 cttagtactg tccgaaggtt ttgctgtcag aaacgccaag ggggatcgcg taggtgagac
       61 tacggtaggt caaagcaatt cattgtctga cgggggcacc gagtccggcc ccgttcgaaa
      121 aaacatagat tgggaaaaaa cctgaaatgg ccaacgaggg gtgcgaaaaa tcagaggtct
      181 aagacccttt tggggcgggg tttaccgatt atgattgatt ttcggcgaaa aggcttgggg
      241 agttgccccc gttgaccgag gctttataat cgcaagaggt atggtttgag tatttgcgac
      301 acggtaccat ggaattgaac tagggagctt ggcccccaaa ttaggagcaa ctgcccgatg
      361 ggaaaagcca tttcattgac tgagcagagt ttcgatcaag aagtcttgca atcggacgtt
      421 ccggttctcg tcgatttctg ggcaccctgg tgcgggccct gccggatgat cgccccgact
      481 atcgaacaat tggctgagga gtttgccggc caggcaaagg tctgcaagct taatgttgac
      541 gaaagcaccg ccattgctgc gcggtacagc gttaccagca tcccgacgat cattattttc
      601 aagaacggcc aagtcgttga ccgttatgtg ggtgcgcagt ccaaagagcg actacgggat
      661 gctcttgtcg aggccacgcg ttgacggtct ttgccataca ccccctgtgc gcgaactgcc
      721 agggagtaat cccggttctg tcgtcgttgc cagacccatc gtgggattgt cactagaccc
      781 cgtgctctgt gaaggggttt cgttccgggc gtgcgcagag gagattggcc tcctcgtcgc
      841 cgatgaattt ttccgtctta ggatcgaaac ggagtttccg gtctagaatc caagcgatcg
      901 cggcggcgtg gcacgcaacg tgcgaatgtc gcatgacatc ctcattggtg accgtcttgg
      961 cacgcgattt gacacaatca aagaagtttc gcgcatgtgc ggagacgtcc aggcctcgaa
     1021 cgcgagtgat ggcttcgggt acttcttttt tcaaggtgtc agggtggacc tcgatcccgc
     1081 cattgtcacc cgtttccacc caaccttcct cgccgacaaa tcggaccgga cacgtgccca
     1141 actctgtaat aaacttcgga gaacgatcac caaatggtgt tttcaggaac tccaggatca
     1201 gtttgacgcc actggcatag gtgcaaacaa ttttgtcacc cattggctca tattccaccg
     1261 gcacggtatt gtcggccccg atcgcccatt ggcacaaatc gacggtgtgt gcgccccagt
     1321 ccaacagtcg ggcaccggag tcgaaatcga aatagccccg ccaaccgccc tggacatact
     1381 tttggttgta cggccgccaa ggtgccgggc ccagccaaag gttccaatcg accacatcac
     1441 ggggcggagt ctgttcccgc ggcagccaac tattgtccag ttggggttcg taaaccgagg
     1501 catacaacgt atggatcttt cctagcttgc cgctgcgagc caactcaaca gcataaataa
     1561 agttggggac gctccgtcgc tgcgtgccgg cctgaaagat tcgctttgtc cgcgaaaaag
     1621 tcttggccag ttcctggcac tttgcaatcg tgatcccgca tggcttctca ctatacacgt
     1681 ccttacccgc ctctgccgcc atgatcgaag ccggcgcgtg ccagcggtcg ccagtggcta
     1741 ccaagacggt atcgatgtcc ttgcggtcaa gtagctcgcg gaagtcccga taggtcacac
     1801 aatcctggtt accataaaac tcatcaacca gctttttgcc ggcctcccgt cggcttgcct
     1861 ggacatcggc gatggccaga catcggacat cgggtaactt aagcatcgct tgtaggtcgt
     1921 acgtggcgcg aggcccaatg ccaatgaccc ccaaagtgat tcgttcactc ggggcaggtt
     1981 cagcagttag tccaagtgcg ctggccggaa ttaacatggg aactccactg aggaccccac
     2041 ccacgacacc ggtgacccca agaaactgac gccgagttgc tttggctttc atcagcagtt
     2101 cctttcctca aatttgacac cacaactcgt aaggtacacg tccaactaaa cccatacttg
     2161 gtgtgtcctc agcctactgt ggccaatcgg gggaatcaag cgatggtcaa atcaatcaag
     2221 ccgtggccat ggatttagag cctgtcccgc tatcttccct tgcccgttcc gcgggagaga
     2281 gattgggggt gagtgtggct cgaatacctg ccggttttct cgatcgactc cgacgatcgc
     2341 ttgccctcac cccaccctct cccggaggga gagggagact gactcttagc acagcgcaaa
     2401 agaaggaggg caccgaatcg tcggtaccct cggagccaat gggagcctca aactgacatg
     2461 tggttgctca gggggaattt ctgattcttc catggaacat catgcttgtg ttgtcggcca
     2521 cgccttggac cgaccgaaat aggtcgccaa gtgcctcgtc cactggccgt ccggggtacc
     2581 aaactaggcc aatggataag ctccaatgtt cgtccatttg cccgtcggct cctgtaccgg
     2641 tgttcggggc caaataggcg aaattcgcct cgagagccaa gctccggtcc aacggcaaca
     2701 cgatatcgcc accgatcagg ccatcgccgt atccggagac accggcccaa aaccgccctt
     2761 cgcctccgcg acaaaagtgc caccgataaa acatggcgta ccgatcgagg acagacaaat
     2821 ccagataccg tctctcctgc tcgttaccat cacgcagtaa cagtaggtcg tccttgacgc
     2881 cgtacatgcc catataaccg atctcgcgct ggtcatcgag gaaaaacgag atctcgtgac
     2941 gtatctggag caagtcggaa tcgacgtagt agtcatcatg catccagtcg aacagcacgc
     3001 tccattgcca acgatcacac tcgggtcggc gaaagagacc ggcggtgaag aacatctggg
     3061 cccggctagc attagtgatc tgatcttgcc cgtaccatgt caaggtttga tgtccggcga
     3121 agtcactgtg gacaacctgc accccgacct gatacccaat attccaaggg ccacccaaag
     3181 gcgctgagta gttaagtccc tcatggaggc cgaagttgcc gtttcgacct aaatcggcgg
     3241 ttcccttaaa gccgtgggca cctgcaaaaa cgctgaggtg ttggatccac gggccaaaag
     3301 gaaaacagcc gtagcacgcc ccttcacaat agccggaacc acagctaaga agattatcct
     3361 ccacggggac gaaagccgaa tcgagatact cgccatcctc cacaccttcg acaggggtga
     3421 taggttcacc aggcggaatc atctcgggca ccggctgtat gttctccgat atgaaccggg
     3481 tacgcggggt ctggattgaa tactcggtag cccgatccgg ctgaagcacc tggcttgtca
     3541 caatgggacg ggagtcgatt accttcccag tggatgtggg acggccgagc acgcggctcg
     3601 accagtaggc tgagggggaa cccgtcgcgc gggtgacata atcggtagcc caggcgattc
     3661 gatctcccac gagcataaag ctcataaagc acgcccagag gagacgagca atcatgtctt
     3721 tgaacatacc attggctccc aaagcactcg attcgtgatt ctccaaaccg agttggcatg
     3781 acaccgcgaa aacactcaat ccgatcgctt cggaatagtt atcgtcatcg gaaaagtcgc
     3841 atctttcggc aaactttgcc taaacggaaa agttttgtgc aggcgaggtt tcgctgtgta
     3901 aatatgggat gacggcgacc tttaggaaga gaattcgcga gaccacaatc tagggacaaa
     3961 agactcagcc acctttggtt gcccgatcca gggcttgttc gagatcggcg atcaaatcct
     4021 ccgggtcttc caagccgatg gaaagcctaa tcaattcgtc tcggattccg tgctttgccc
     4081 gaaaggctgc atcatagctg gcgtgggaca tggtggccgg ctgctcgatg agcgattcca
     4141 ctgcccctag gctcacggca agctggaaaa tctgggtaga tttggcgaac ctctggacca
     4201 ggccggcgtc cccatcgagt tccaagctga cgatagcgcc gaaccccccc gacatttgcc
     4261 gggcggcaat ctcgtgaccc ggatggtctg ccagacccgg gtaatagacc ttacggattc
     4321 tgggatggtt atgaaagaag tctgcaatct gatgggccgt ccgagactgt tcccgaacgc
     4381 gtaagtcaag ggtcttaagg cctcgatggc aaaggtaggc ctcaagcggc gccatgacag
     4441 ccccagtagc attttggatg aaatatagcc gcttgaacag ttcagagtcg cgcaccacca
     4501 ggacaccccc gaggacgtcg gtatgccccc cgatgtactt ggtggcggaa tgcatgacga
     4561 tgtctgctcc cagctcgagg ggacgggtca agacgggggt cgcaaatgtg ttatcgactc
     4621 ccaagatggc gttgtgccga tgagcaatct cggcgcaggc tgcaatatcg gtaatggaga
     4681 gcaacggatt cccgggcgtt tcaatccaca aaaggcgggt tcttggggtg aaggctgcct
     4741 ccacggcgtc cagatcgttt gtcgggacca tcgttacggt aatgcccgaa cgattgatga
     4801 tcttgtgaag taggcggtag gtccctccgt acacgtcgta acctgttacg atatgatcgc
     4861 cgcattccag gagcatggtc acgcaatgga tggccgccat tccggaggaa aaagccaggc
     4921 caccgacacc ggattccagc tcggctatgg catcttcaaa gcacttccgt gttgggttac
     4981 cgctgcggga atagtcaaac tcgccccact gacctggtgc gggctgcgcg aaggtcgtcg
     5041 aaagatagat tgccgggact aaagccccgg tcgatcggcc tggcggcgta agatggatcg
     5101 cctttgtgcg aaacttcatg gaaagttcgc ctattaagtc aagtccactc t
//
LOCUS       DAOSUT010000229         5141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7192_length_5141_cov_8.058985, whole genome shotgun sequence.
ACCESSION   DAOSUT010000229 DAOSUT010000000
VERSION     DAOSUT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_5141_cov_8.058985"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..755)
                     /gene="coaD"
                     /locus_tag="PLD05_14685"
     CDS             complement(246..755)
                     /gene="coaD"
                     /locus_tag="PLD05_14685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOP78733.1"
                     /translation="MSELNSRVAVYPGSFDPITLGHLNVIERASRLFDQLIIGIGVNI
                     HKFPMFDAKEREELIRLSTPHLTNLEIRTFRGLAVNFVKECGAKIMIRGVRPITDIAA
                     ELTMMMANRRLAPDVETLFMIADGELAHVSSSLIKEIAPVASDEELSRFLPDPVVRAL
                     RAKLQASRR"
     gene            893..2668
                     /locus_tag="PLD05_14690"
     CDS             893..2668
                     /locus_tag="PLD05_14690"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78734.1"
                     /translation="MGSTKIVLGASINLTRSAMWLSVLVMTWGVLGSSVYAQRQMDKL
                     DRGLVAVQAGPGEVYVSWRLLKDDPKDLAFNVYRQAEGTAQAVRLNDRPISKTCDFTD
                     RGVSRGRYVYSLRAVKGGKEEQSGPSFTLEVTDKPQPYVSIKLQGDHTFQKVGIADLN
                     GDGSLDIVIKQPNQNIDPYEKYWKPSPSTYKLEAYTLDGKMRWRYDLGWAIEQGIWYS
                     PYIVYDLDGDGKAEVAVKTGEGDPRDSDGRVQSGPEYLTILDGETGKPVTRTDWPSRE
                     LFPSYNYASRNQLAVAYLDGKTPSLIVLRGTYNVMLAIAYRYEKGRLTEIWRFDNRGM
                     ERKFQGQGAHWTHAADVDGDGKDEIILGSVVLDDNGQPLWSTGLGHPDHAYVGDLDPA
                     RPGLEIYYGIETRQQKNGMCMVDARTGEILWGYDGPTRHVHSHGMCSDIDPRYPGAEC
                     YSADTDANKDFAWAKLWSAKGEVISEENISGFGPRVAYWDADPQRELVMGSRIRKYHP
                     SQELSPRLEGSFVATVDLLGDWREEIITTLPGEMRIYVTTIPASDRRPCLLQDPIYRL
                     DVAMAAMGYYQCPMTSFDLASQKAE"
     gene            2746..3540
                     /gene="larB"
                     /locus_tag="PLD05_14695"
     CDS             2746..3540
                     /gene="larB"
                     /locus_tag="PLD05_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOP78735.1"
                     /translation="MLNIEQLRILTEQLKRGQIDVEGFVQACQKWGIADVGEAQVDID
                     RQRRCGFPEVVYGEGKSVEALRKIFHALCENGVDVLATRVSQEKADALLPEFPNARYN
                     ALAKTFRIAREAKTEDQPRGIVTVVTAGTSDLPVAEEARETALWTGAEVRLVQDVGVA
                     GPHRLRANLHLLQDADAIVVVAGMEGALPSVVGGFVSCPVIGVPTSVGYGASFGGVSA
                     LLSMLNSCAANVVVVNIDAGFKAGYVAGLIAKNSALSRAKSQSAAK"
     gene            3537..4439
                     /locus_tag="PLD05_14700"
     CDS             3537..4439
                     /locus_tag="PLD05_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOP78736.1"
                     /translation="MIQVPFEDNPHLPVLPIRPRDAHKGHFGTVLIVGGSLGMTGAAG
                     LAGMAALRGGAGLVRVAVPEPVLPIVAGYEPSYTTIPLPADSAGRISYQALKKIRVEA
                     ELADWVALGPGLGRSWGLSRLVSQLYRDLPNPLVVDADGLNALVTLAPDIPPANGPRI
                     LTPHPGEFRRLVGPGADPRAPLADRWQLAQHYAAVWDAVVVYKGHPTLVTDGKRRYFN
                     PTGNPGMATGGSGDVLTGLIAALGAQGLDSFSAAQLGVYLHGLAGDLGAKDLGEESLI
                     ASDLLRYLPLAFQKYRETSSKTNG"
     gene            4565..4978
                     /locus_tag="PLD05_14705"
     CDS             4565..4978
                     /locus_tag="PLD05_14705"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOP78737.1"
                     /translation="MQIYAYLADLVVAIHLAYVLTVLVGLVLILIGWLAKWRWIRHFW
                     LRLIHLLMIAVVAFQAWIGVICPLTHLENYLRKLAGQSGYSGTFIGHLLESLLYYEAP
                     PWVFVFAYTLVAVLILSTLILVPPNWPRRLKPSQQ"
BASE COUNT         1119 a         1372 c         1493 g         1157 t
ORIGIN      
        1 tgcgatgctc tgtcactcgc gtcctttctg gatggtgtcc gcccccaaag cacgtgacga
       61 ggcgttttcc tcaagaagat ttgctgctgg agcgatagag gccgcaatgg accgcggcgc
      121 gaggtcgtac ccaattcaac gacagaggct cacgcactgg tatggtcgtg gaaccctaaa
      181 gaactcagca ctcgtttacc aacgcgaaga cctttcagta aatctttccg acagtttagc
      241 ttggcctatc gccgtgaggc ctgcaatttt gcccgtaaag cccggacaac aggatcaggt
      301 agaaatcggg agagctcttc gtcgctcgca acgggggcaa tttccttgat caacgaactg
      361 gagacgtggg caagctcacc gtcggcaatc atgaacagtg tctcaacatc cggtgccaac
      421 cgccggttgg ccatcatcat cgtcaattcc gctgcgatgt ccgtaattgg ccgcacacca
      481 cgaatcatga tcttggcgcc gcattccttc acgaaattga cggccaagcc ccgaaacgtc
      541 cgaatctcca ggttcgtcaa atggggcgtc gaaagtctta tcagctcttc ccgttccttg
      601 gcgtcaaaca tcgggaactt gtgaatgttg acaccaatgc cgatgatgag ctggtcaaac
      661 aagcggctgg cgcgctctat gacatttagg tgacccagcg tgattgggtc aaaggaaccg
      721 ggatagacgg caactcgcga attcagctca gacataagat aagctcctca ggcggtagca
      781 aaacgcttct ttggtgatta tattacgaaa gaacttctgt gccgagaggt aagaggcatc
      841 gataggtttg acgacaatct tgaattccca caattgcggg gaggaggatg caatgggttc
      901 gacaaagatc gttcttggcg cttcgattaa cttgacgcgg tccgcaatgt ggctcagcgt
      961 tttggtcatg acctggggtg ttctggggtc gtcggtttat gcccagcgac aaatggataa
     1021 actcgaccgc ggcttggtag cagtgcaagc tggtcccggg gaagtttatg tgagctggcg
     1081 gcttctgaaa gacgacccga aggacctggc ctttaatgtt taccggcaag ctgagggaac
     1141 agcccaggcg gtccgcttga acgatcggcc catttcaaag acctgtgatt ttaccgatcg
     1201 cggggtctcg cgtgggcggt acgtctattc tcttcgagcc gtaaaaggcg gcaaagaaga
     1261 acagagcggt ccatcattca ccctggaagt gacagataaa ccccaacctt atgtgtcgat
     1321 caagctacaa ggtgatcaca cgtttcaaaa ggtaggtatt gccgacttga atggcgatgg
     1381 atcactggac atcgtcatca agcagcccaa tcaaaatatt gatccctatg aaaagtattg
     1441 gaagccgagc cccagtacgt acaaattaga ggcttacacc ttggatggca aaatgcggtg
     1501 gcggtacgat cttggctggg ccatcgagca gggcatttgg tactcgccct atatcgtgta
     1561 tgacctcgat ggtgatggaa aggccgaggt tgcggtgaaa accggtgaag gtgacccgag
     1621 ggattccgat ggccgtgtgc aaagtggccc agagtatctg acgatcttgg atggagagac
     1681 aggcaagcct gtgacacgaa cggattggcc gtcccgagag ttattcccct cgtacaatta
     1741 cgcttcccgt aatcagcttg ctgtggccta ccttgacggg aaaactccat cattaatcgt
     1801 cttgcgcgga acatataacg tcatgctggc cattgcttat cgctatgaaa agggtcggct
     1861 cacggaaatc tggcgctttg ataaccgtgg gatggagcga aagttccagg gccagggtgc
     1921 ccactggaca cacgctgccg atgtcgatgg tgacggtaaa gacgagatta tcctagggtc
     1981 agtggtcctt gatgataatg gccaaccatt gtggtcaaca ggtctgggac atccggacca
     2041 tgcgtacgtc ggcgatctcg atccggcacg gccaggactg gagatttatt atgggatcga
     2101 gactcgccag cagaaaaacg gcatgtgcat ggtcgatgcc cgcacaggtg agattctttg
     2161 gggctacgac ggtccaacgc ggcacgttca tagtcacggg atgtgcagtg atattgatcc
     2221 ccgttatccg ggcgccgaat gttacagcgc cgatacggac gccaacaagg atttcgcttg
     2281 ggcaaagttg tggagcgcca aaggtgaagt catcagtgaa gaaaacattt ccggttttgg
     2341 cccccgcgtg gcctactggg acgccgatcc gcagcgggaa ctggtgatgg gctctcgaat
     2401 ccgcaagtac cacccgtcgc aagagctttc acctcgtcta gaagggagtt tcgtggccac
     2461 ggtcgatctt cttggcgact ggcgtgaaga gatcattacg acgctccccg gcgaaatgcg
     2521 gatctacgtg accacgatcc ccgcaagcga ccggcggccg tgcctgctcc aggatcccat
     2581 ctatcggctg gatgttgcca tggcggcgat gggatattat cagtgcccca tgaccagttt
     2641 cgatttggca tctcaaaaag cggagtgacc gtcattttag gagacgatcc ctgatcgttc
     2701 aaaaagggta tttgcgacat tgttgtcaac aaatcactgc caatcatgtt aaatattgag
     2761 caactacgga tactgaccga gcaattgaag cgcggccaga tcgacgtgga aggttttgtc
     2821 caggcgtgcc aaaagtgggg aatcgccgat gtcggtgagg cccaggtgga catagatcgc
     2881 cagcggcgct gcggattccc ggaggtggtc tatggcgaag gcaagagtgt cgaagccctg
     2941 cgcaagatct tccatgccct gtgtgaaaac ggtgtcgatg ttctcgccac ccgagtttct
     3001 caggagaagg ccgacgcctt acttccggag ttccccaacg cccgatacaa cgctttggcc
     3061 aaaacattcc ggattgcccg ggaggcaaag actgaggacc agccgcgggg aattgtgacc
     3121 gtggtaacgg ccggaacaag cgatttgccc gtcgccgaag aagcccgaga gacagccctg
     3181 tggaccggtg cagaggttcg attggtccag gatgttgggg tggctgggcc gcaccgcctc
     3241 cgggcaaatc tgcaccttct tcaggatgcc gacgccattg tcgtcgtggc gggcatggaa
     3301 ggggcgctgc ccagcgtggt gggagggttc gtttcctgtc ctgttattgg tgtacccacg
     3361 agcgttggct acggggccag tttcggcggc gtctccgcac tgctgagcat gctgaatagc
     3421 tgcgcggcca atgtggttgt ggtgaacatc gacgccggct tcaaagctgg gtatgtggca
     3481 ggtttgattg ccaaaaatag tgccctgtct cgcgcaaaat cccaatctgc cgcaaaatga
     3541 tccaagtacc ctttgaagac aatccccact taccggtatt gccgatccgt ccgcgagacg
     3601 cccacaaagg tcacttcggc accgtgttaa tcgtcggcgg aagtctcggt atgacggggg
     3661 cggctggcct ggctggaatg gcggccctac gaggcggtgc cggattggtc cgtgtcgcgg
     3721 ttcccgagcc ggtgttaccg attgtcgccg ggtatgagcc gtcgtacacc accatccccc
     3781 ttccggccga ttcggcgggt cgtatttcct atcaggctct gaagaagata cgtgtggagg
     3841 ccgaactggc ggattgggtg gcactaggac cgggactggg ccgttcgtgg gggctatcgc
     3901 ggcttgtctc ccagctatac cgcgacttgc ccaacccatt ggtggtggac gccgacggcc
     3961 tgaatgctct ggtgacactg gccccggaca taccgcccgc caacggcccg cggattttga
     4021 cgccacatcc aggcgaattc cgccgattgg ttgggccagg tgccgatcct cgagcgccac
     4081 tggctgatcg ctggcagttg gcccagcatt acgcagccgt ctgggatgcg gtggtggtct
     4141 ataaaggcca tccaaccttg gtgacggacg gaaaacgtcg ttattttaac ccaaccggga
     4201 atcctggcat ggccacgggt ggatcgggag acgttttaac cggtctcatt gccgccttag
     4261 gagcgcaggg actcgatagc ttttctgccg cccaactagg cgtttatctt cacggtttgg
     4321 ctggggacct gggcgccaag gacctcggcg aagaatcgct gatcgcttcg gatctcctgc
     4381 ggtacctccc gctggcattt caaaagtatc gagagacatc ctccaagacg aacggataaa
     4441 cggaagggcc cgatcaccgt ttcgccggct tcgcttgcca accgaggcac ggtcgcctgg
     4501 aataaatccc ccgccaaatg gatcgaaatt atagctccat acttgacgcc gggatgctgc
     4561 ggatatgcaa atctacgcct atcttgccga tcttgtggtg gcgatccatt tggcctacgt
     4621 gctaacggtg ctcgtcggcc tggtcctcat cctcatcggc tggttggcca agtggcgatg
     4681 gattcgccat ttttggctcc gcttgattca cctgttaatg attgccgtcg tcgcttttca
     4741 ggcctggatc ggtgtgattt gcccgttgac ccaccttgag aattatttgc ggaaactagc
     4801 cggacaatcc ggctacagcg ggacgtttat cggccacctg ttggaatcct tgctctatta
     4861 cgaagcaccc ccgtgggtct ttgtgtttgc ctacaccttg gttgcggtgc taattctttc
     4921 cacactaatc ctggttcccc ccaactggcc aaggcgtctt aagccttctc agcagtaggc
     4981 cggtgttgat tggcttttat cggttcagcc aagtctcaaa aagggaccga gccgtcttct
     5041 ggaatatccc ggcaacttga gggattcaaa aagaacgcca tggcttttga cagaggtggg
     5101 gcttcctcgc cccgaagcca tttctcaata gcacaatttg c
//
LOCUS       DAOSUT010000230         5138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7198_length_5138_cov_9.554987, whole genome shotgun sequence.
ACCESSION   DAOSUT010000230 DAOSUT010000000
VERSION     DAOSUT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7198_length_5138_cov_9.554987"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1475
                     /locus_tag="PLD05_14710"
     CDS             <1..>1475
                     /locus_tag="PLD05_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78738.1"
                     /translation="EEAKPTEKEKPEAEKPTEKPKEPEKPAEEKKPIAAEKPAAPAVT
                     TQAQPSKHKVGRELVKIEKSYTVTFQPKQAAEIKLDNKSWSTFRVVEAVEHGRQVRKG
                     DVLVRFEREEFDRALVDQQRAVRSAELALQDAERLLALLEKTVPFDLATAERNLQITK
                     EDTERYFAKERDLTIRTYEMNLGSAKMSLESAEEELRQLEKMYKADDLVEETEEFILK
                     RQQFAVERARFMLELEQDRYQEFHEKGLARLDESIRRDLERTMLVTERSRISLPSLLA
                     QQRISVEQLRTDLERAKDRLSKLQADQAFMEIKAPCDGIVYYGEFNRGEWGGATTLMS
                     KLRPGGSPSKGDVIMTIVQVPPQTVRFSVGEADLRWITPGLEGTVKPKSFPQVSLPAC
                     VTEVASIPVAADRFDVVAEVTLPKKPLPLVPAMTGTIRFNVYVKEDALVVPAKAVFTD
                     ELDPDKRYVYVLVDGKPEKRSVKVGEQVRDKVEILSGLRDG"
     assembly_gap    1476..1575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1576..2622
                     /locus_tag="PLD05_14715"
     CDS             <1576..2622
                     /locus_tag="PLD05_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOP78739.1"
                     /translation="GSWGSARNTKSLNIYAPVPGAHQAFRAAVTSLCVSALIDVKDSR
                     SEVQEAIDRGEQWLLENLGNVRRATPDAIYNTWAHAYALQALTRLIQRHKGQPERQAA
                     LRELMEGQLDRLIRYECLNGGWAYYDFDYGTQKPGSSTISFVTATVLVALHDAKQVGL
                     TVPPKIIDRAKDSILRQRKPDFSYCYGEYLKMVPMHPVNRPAGSLGRSQACNLAMRLW
                     GDESVTDEVLRAWLDRFFARDGWLDLGRKRPIPHESFFAIAGYFFYYGHYYASRCIEL
                     LPQKERATYYNHLAAILIDLQEKDGSWWDYPLYDYHQQYGTAFALLSLQKCRIEGRKA
                     AQAEGSSSLAARSQ"
     gene            complement(2750..4498)
                     /locus_tag="PLD05_14720"
     CDS             complement(2750..4498)
                     /locus_tag="PLD05_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOP78740.1"
                     /translation="MHSVGRFLVLLSSFITLWHASLTMGQAVPSIWYEDMGTARRVAA
                     ETNRLLLIHFWAEWCRPCRQMEAEVFSRPDVLSALATNYVAVKVNFDRAPELVREFRI
                     ESIPTDVILTPNGELLQKFVGGSSAERYIERISQVAYTYQQRRTMLARTNTPSATPPL
                     TPSYATSPWSSPSPQGTAAATRPVDQPPTMAMNAGVGLPQSGPGGMPPSSQGSYFSGQ
                     PLASAPAPVGPGQIPAGPSGFGQPQNQVLPQPPAGLSPWSNSSQLATTSTPLAPPPDT
                     SFQASPSPPQSIQGYATSSPPSSANVGPSQSFGPPPQVAQGPSSSPPMMEAQPPANSM
                     ASTILARQSPKPKVEIPPGNPPLALEGYCPVSLTDKQRWVLGNPRWGARHEGRTYLFA
                     GPEEQQKFLANPDRYAPVLSGMDVVQLVEATQLTEGRREHGAWFGGRVYLFSSEESYQ
                     KFSLDPYRYINALPQAVARLSQQIQGEASLTTSPGNVAGTNVPPQQPPVGMGQLPVGN
                     PPLRPTVSSPMAPAISPPTSPPVDTVGGVPTASATSWAPPPTVNIQLPPSVQAVANGT
                     ASPDQPRWPVAQRPRW"
     gene            5065..5138
                     /locus_tag="PLD05_14725"
     tRNA            5065..5138
                     /locus_tag="PLD05_14725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5099..5101,aa:Met,seq:cat)
BASE COUNT         1127 a         1347 c         1502 g         1062 t
ORIGIN      
        1 gaagaggcta aacccacaga aaaggaaaaa ccggaagcgg agaagcccac cgagaagccg
       61 aaggagccag agaaacctgc cgaggagaaa aagcccatag cggcggaaaa accggcggca
      121 ccagcggtga ccactcaagc tcagccatcg aagcacaagg tgggacgcga gctggtcaag
      181 attgagaaga gttacaccgt tacattccag cccaagcaag cggctgagat taagcttgat
      241 aacaaaagct ggtccacatt tcgtgtcgtg gaggccgtag aacacgggcg gcaagtgcgg
      301 aaaggtgacg tcctggtccg ctttgagaga gaggagtttg accgtgcgct cgtggatcaa
      361 cagagggcag ttcgctcggc ggaattggcc ctccaggacg ccgaacgact actggctctt
      421 ttggaaaaga cggtaccgtt cgatctggcc acggcggagc ggaatcttca gatcactaaa
      481 gaggacacgg agcgttattt tgccaaagag cgcgatctga ccatccgcac ctacgagatg
      541 aaccttggat cggccaagat gtcactcgag tcggcggaag aggaacttcg ccaattggaa
      601 aagatgtata aagcagatga cctcgtcgaa gagaccgagg aatttatcct caagcgacaa
      661 cagtttgccg tggaacgagc tcgtttcatg cttgagctgg agcaggaccg ctaccaggag
      721 ttccacgaga aagggcttgc ccggcttgac gagtccatac ggcgtgattt ggaacggacc
      781 atgttggtaa ccgagcgttc ccggatcagt ttgccctcgc ttcttgctca acaacggatc
      841 agtgtggagc aactgcggac cgatttggaa cgggcaaaag atcgcttaag caaactgcag
      901 gctgaccagg cctttatgga aatcaaagcc ccttgcgatg gcatcgtcta ttatggggaa
      961 ttcaaccgtg gcgaatgggg tggcgctacg acgcttatga gcaagctgcg acccggtggg
     1021 tcaccgtcaa aaggcgatgt catcatgacg attgttcagg tgccgccgca aaccgtgcgt
     1081 ttctcggtgg gagaagccga tctccgttgg attacccccg gcttggaagg caccgttaaa
     1141 cccaagagtt ttcctcaggt gagcctgccg gcttgtgtga cggaggtggc ctcgatccct
     1201 gtggcggccg atcggtttga cgtggtggcc gaggttacgc ttcccaaaaa gccactgcct
     1261 ttggtcccgg cgatgaccgg cacaatccgc ttcaatgtct atgtcaagga ggacgcgctg
     1321 gtggttccgg ccaaagctgt cttcaccgac gaacttgatc cggacaaacg atacgtttac
     1381 gtactcgttg atgggaagcc tgaaaagcgt tccgtgaagg tgggcgagca agtccgagat
     1441 aaagtggaaa tcctcagtgg actgcgcgat ggcgannnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnggttc ctggggttcg gcacggaata ccaagtcgct caatatctac
     1621 gccccggtgc cgggtgctca tcaggctttt cgcgcggccg tgacttcgct ttgcgtttcc
     1681 gccctcatcg atgtcaaaga ttctcgatcg gaagttcagg aggcgatcga tcggggcgaa
     1741 caatggcttt tggagaatct tggcaatgtt cgccgggcaa cgcccgatgc catttacaat
     1801 acttgggccc atgcttatgc cttgcaagct ttgacacggc tcatccagcg acacaaaggc
     1861 cagcccgaac ggcaggcagc cttgcgtgaa ttgatggagg gacaactcga ccggctgata
     1921 cgttacgaat gcctcaatgg cggttgggcg tactatgact ttgattacgg cactcaaaag
     1981 ccgggtagtt ccaccatcag ctttgtgacg gccaccgtac tggtggcctt gcacgatgct
     2041 aaacaggttg ggttaaccgt tccaccgaag attattgacc gcgccaaaga ttctattctg
     2101 cggcagcgga agcccgactt tagctactgc tacggcgagt atctcaagat ggtgcccatg
     2161 cacccggtca atcgcccggc ggggagtcta ggtcgatccc aggcctgcaa tcttgcgatg
     2221 cggctttggg gggatgagag cgttaccgat gaggtcctcc gggcatggct ggatcgtttt
     2281 tttgcccgcg atggctggct cgatctcggt cggaaacgtc ccattcccca tgagtccttt
     2341 ttcgcaatcg cgggatactt tttttactac gggcactatt acgcttcgcg ctgtattgaa
     2401 ctgcttccgc aaaaagaacg ggccacctat tacaatcacc tagcggccat ccttatcgac
     2461 ctccaggaaa aagacggctc ttggtgggat tatccgctct acgactatca tcaacagtac
     2521 ggcacggcgt tcgctttact atccttgcaa aaatgccgga tcgaagggag aaaagccgca
     2581 caagcggaag gctcttccag cctcgccgcc cgcagccagt gagcccctct caagaaccgg
     2641 cttagcctcc ttcggtagag cggccaagtg gtcgtcgcta ccgacggccc tcacgtaaac
     2701 ggcacgcttc gctgcaaaga tggtcgcttt ttccgagagt gcttttccat caccaacgcg
     2761 ggcgttgtgc caccggccag cggggctggt cgggtgaagc cgttccattg gccacggcct
     2821 gaacggacgg cggaagctga atgttgaccg tcggcggcgg ggcccacgaa gtcgcacttg
     2881 ccgtcggtac cccacccact gtatcaacag gtggacttgt cggggggctg attgcgggtg
     2941 ccatcggaga tgagacagtc ggtcttaacg ggggatttcc cacaggaagt tgacccatcc
     3001 ccacaggggg ctgctgcgga ggaacattgg tacccgctac gttccccggc gaggttgtca
     3061 aagacgcctc tccctgaatc tgctgtgata gacgggcaac agcctggggc agtgcgttga
     3121 tgtaccgata gggatcgagg ctaaacttct gataggactc ttcgctgcta aagaggtaaa
     3181 cgcgtccacc aaaccatgcc ccatgttcgc gccggccttc cgtcaattgg gtggcctcaa
     3241 cgagttggac cacgtccata cccgaaagca ctggggcata acgatccggg ttggcaagga
     3301 acttttgttg ctcctcgggc ccggcaaaga gataggtgcg accttcgtgc cgtgcccccc
     3361 atcgaggatt tcctaagacc cagcgttgct tgtcggtcaa actcaccggg caatatccct
     3421 cgagcgccag gggtggattg ccgggtggga tttcgacctt aggttttggt gactgacgtg
     3481 ccaggatggt gcttgccatg gaattggcgg gcggctgtgc ttccatcatt ggcggcgatg
     3541 acgatggccc ttgggccacc tggggtggag ggccaaaact ttgtgacggt cccacattgg
     3601 ccgacgatgg tggcgaagaa gtcgcgtagc cttgtatgct ttgaggagga ctgggcgacg
     3661 cctggaacga tgtgtccgga ggtggggcaa ggggtgtgct tgtcgtggca agctggctac
     3721 tgttactcca tggggacaaa cctgctggcg gctgggggag aacttggttt tgaggctggc
     3781 caaagccaga aggaccagca ggaatctgac cgggccccac cggagcgggg gcagaggcca
     3841 atggttgccc actgaagtaa ctaccttggc tactaggagg cataccaccc ggtccagact
     3901 gcggcaaccc gacaccggcg ttcatggcca ttgtcggggg ttgatccacc ggccgcgtgg
     3961 ctgctgcagt accctggggt gacggtgacg accaagggga ggtagcgtag ctgggcgtaa
     4021 gcggcggtgt tgccgacggg gtatttgtcc ttgcgagcat tgtacgccgt tgttgatatg
     4081 tgtacgcaac ctggctgatc cgttcgatgt agcgctcggc cgaggaccca cccacaaact
     4141 tttgcaggag ttctccattg ggcgtaagaa tgacatcggt cgggatcgat tcgattcgga
     4201 actcccgtac aagttctggg gctcgatcga aattgacctt caccgccaca taatttgtgg
     4261 ctagggcaga taggacatcc gggcgagaaa agacctccgc ctccatttga cgacagggac
     4321 gacaccattc cgcccaaaaa tgaattaaaa ggagccgatt cgtctcagcg gcaacacgtc
     4381 gggccgtgcc catatcctca taccaaattg aggggaccgc ctggcccatg gtaagggacg
     4441 catgccagag tgttataaac gaacttaata gcaccagaaa ccggccgacg ctatgcatcg
     4501 gaacaatcct cgtttgttgg agtgacgaaa cgaccaacga cgcccaaaac tgtggcgagc
     4561 atcgtcaact taaattgccc acacaagctc cctaaactcc aacagccaaa aagggcatca
     4621 cacacctaat gaccgccctt tttttatcgg actgatgggg tcagtcaatt ttgtgaaaat
     4681 gttccgaatt tgccggaaaa ccgttttatt gcagttgcaa caggcgcggc tgacgctttt
     4741 ccttgtggac cggtgaagta tccttttcac tgctatcttt tcctgattgc cccgttttgc
     4801 cagacgaaat gggacagcag gtgaggacca accgccggtg agggcgttag ctggtatgaa
     4861 gtggatgttc ataagtcccg ctaccttgtg cgcctgaatg cgaaaaggtt tcccgaactc
     4921 ggggtcacgg cgatttaagc tcggtgagtt cgactcgaac agagttggta agtcaaaagg
     4981 ggggaggtta cgccattccg cccccttgtc cgcatctggt cgagaagtta tgtttcagta
     5041 aaggccctcg gtcggccaag acctgccggc gtagctcagt tggttagagc agcggaatca
     5101 taatccgcgt gtccggggtt cgagtccctg cgccggca
//
LOCUS       DAOSUT010000231         5100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7253_length_5100_cov_10.963330, whole genome shotgun sequence.
ACCESSION   DAOSUT010000231 DAOSUT010000000
VERSION     DAOSUT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7253_length_5100_cov_10.963330"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLD05_14730"
     CDS             complement(<1..1037)
                     /locus_tag="PLD05_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78741.1"
                     /translation="MSQSKIVKKDNVGKILDQAMDAGGGILRLTPTWVPRSFLHPGRR
                     IKLATEDLYAYGAHRGGIDERWFASTTEAANEGRNWDEGLSWVRFNGERFLLRDAVEE
                     GQGRIVGPAIWEKYHRWPVYSKFFDNAGPIPHHMHQTHEFAKLTGQEGKPEAYYFPPQ
                     LNAAENHFDYTFMGLEPGTTKEDVKRCLENWNKGDNGILDLSRAYRLKPGTGWLIPAG
                     VLHAPGSRCTYEPQWGSDVFGMFQSIVEGRYVPWDLLVKDVPKDKHHDLDFIVSQLDW
                     EKNVDPYFKEHNYLEPIVDEENSGDGYTDRWIVYGLVDGQQLFSAKELTVMPGAKCVL
                     RDPGASGWITV"
     gene            1349..3019
                     /locus_tag="PLD05_14735"
     CDS             1349..3019
                     /locus_tag="PLD05_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOP78742.1"
                     /translation="MGNVWVCQYCGCRIIVRSAKPAVIKVCPRCGQGSHSSQNAIPPE
                     QSSESFPPLVTESYPGRDRRSKARRRRPFFGSSYGFGLAIAGGMVVIGAAVLAIWLAG
                     GFSGGDGARQPRSSSQPKSHGETIPPPLEWTSRQVFVAKTLDQVKEAVVKIEIPLEGG
                     AAIETGTGFFIDKRGWIATNYHLVQNMNNRAYVRSAGGQTYRIAGIVAEAPAMDLAIL
                     ELAERPVRMMILDLSYNDTPRLGTEVYAFGHPYNVDFSLSRGVVSRVLTTSELIQNFP
                     DHIVTKIKSPPAMIWIQHDARISPGNSGGPLLDGTGRVIGVNTFVHKLAQYGFASHIR
                     YLRELAQGASGVVRSLPEPAPPRLEGIASESVVDLRQISPLWQFAQSIMWTPQSLEQY
                     QKLAEWARIANIVKLLQIQGKIPEGVPPAVLDQAAVEAENLFKTLTPDHISQEARDRL
                     NELATQIGFQPLNGIVFVAQLIAEPSPGMVLLTFGSENQHALVRLRPDRPDVRIGAEV
                     FVAGLVSPQAAEIRIGQEGRQERVPLIWGMFLLNLEKPSQTASAEAAR"
     gene            complement(3016..3801)
                     /gene="trpC"
                     /locus_tag="PLD05_14740"
     CDS             complement(3016..3801)
                     /gene="trpC"
                     /locus_tag="PLD05_14740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOP78743.1"
                     /translation="MASILDRIVATKREEVERAKIFAPEEHLRRMVEQQPPPRDFLGA
                     IRNAPNIALIAEVKKASPSKGVIRENFDPVAIARIYEKHGATCISVLTDEPYFQGHIE
                     FLRWVKESVQIPVLRKDFILDSYQVLEARAYGADAVLLIAECLTTEQLTRLYHEILAW
                     GMTPLVEVYEPENLPRVIDLGASLIGINNRNLQTFEVDLEHTIRLRAKIPSDRLVVGE
                     SGIQTHEDVDRLRRAGVNAILVGESLMREADIGLAVDRLLGKS"
     gene            complement(3941..4729)
                     /locus_tag="PLD05_14745"
     CDS             complement(3941..4729)
                     /locus_tag="PLD05_14745"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOP78744.1"
                     /translation="MQRREFLASSMIGGVGAIMAMSELSQAVEGAGGPKQLLELRRYQ
                     IASPEKRDRLEKFFAEVAIPVWNSLGIQPVGVFRYLGEEDPSLFVLLPHNSAETVIRE
                     MAALLADPKAQSVGKDVLYDPFDDPTYQRVESSLLLAFDGVPKVQVPTTAESRVFQLR
                     IYESHNLTKHVKKVEMFNTGGELEIFYRCKMNPVFFGQALIGSRLPNLTYMLGFDNEA
                     AQKEAWAAFLKDPQWLELRDKPEYAQTVSNITNIVLRPSPVSQI"
BASE COUNT         1102 a         1469 c         1396 g         1133 t
ORIGIN      
        1 tggacggtga tccagccact ggcacctgga tcgcggagaa cacacttggc cccgggcatg
       61 accgtcagct ctttggcgga gaagagctgt tgaccatcca ccaagccata gacaatccag
      121 cggtcggtgt agccgtcgcc agagttttct tcatccacaa tgggttccaa gtagttatgt
      181 tccttgaagt acggatcgac attcttctcc cagtcgagct ggctgacgat gaaatcgagg
      241 tcgtggtgct tatctttggg cacatctttg accaagagat cccacggaac atatcgccct
      301 tccacgatgg attggaacat gccaaacaca tccgatcccc attgaggttc atacgtgcag
      361 cgggagccgg gcgcatgcaa cacacctgca gggatcagcc agcccgtgcc cggcttgagg
      421 cgataggccc gcgagagatc gagaattccg ttatcccctt tgttccagtt ctctaggcag
      481 cgtttgacat cctccttggt tgtccctggt tctaggccca tgaatgtgta atcaaagtga
      541 ttttccgcgg cgttgagttg cggcgggaag taataggcct ccggcttacc ctcctggccg
      601 gtcagcttgg cgaactcgtg tgtctgatgc atgtgatgag ggatgggccc agcgttgtcg
      661 aagaacttcg aatatacagg ccagcggtgg tacttttccc aaatggcagg cccgacaatt
      721 cgcccctggc cttcctcgac cgcgtcgcgc aggagaaacc gttcgccatt aaagcggacc
      781 cagcttaggc cttcgtccca gttgcggcct tcattggcgg cctccgtcgt actggcgaac
      841 cagcgttcgt cgataccacc gcggtgagcc ccatacgcat acaaatcctc agtggcaagc
      901 ttgatccgac gaccgggatg gagaaaactt cgcgggaccc aagtcggtgt caatcgcagg
      961 ataccaccgc ccgcatccat ggcctgatcc aaaatctttc cgacgttgtc tttcttaaca
     1021 atcttcgact gggacattgc aggcgcgctc ctccaataag acctaccctc ggcgttgaga
     1081 ataacgccat caacggaacc gtaccgaacg acgcggacgc cacgccgtcc taccacgcca
     1141 cactgatatc gcccattttt atccattgcc ccatcgtaaa gcaagcactt gagaatctca
     1201 cccgtagaat tccgacagga ctagcgacgc tcaatggaga tccatcgacc cgcgccctgt
     1261 tgctcgttgc cgaccaaatg ggtggtcaga accctggcga ggatccagcc aaatcggtag
     1321 tctgaatagg cagagagaat gtgagctcat gggcaatgtg tgggtgtgcc agtattgtgg
     1381 atgccgtatc atcgtccggt cggcgaaacc ggcggtgatc aaggtttgtc cccgttgcgg
     1441 gcaagggagt cattcttccc aaaatgccat accaccggag caatcatcgg agtccttccc
     1501 accccttgtg acggagtcct acccaggacg tgatcggcga tcaaaagccc gtcgccggcg
     1561 accctttttt ggctcaagct acgggtttgg tcttgcgatt gccggtggca tggtcgttat
     1621 cggggcggcc gtgcttgcaa tctggcttgc cggtggcttc tccggaggcg atggggcgag
     1681 gcagccgcgg tcatcgtccc agccgaaatc gcacggcgaa acgatcccgc ctccgttgga
     1741 atggacctcg cggcaagtct ttgtagccaa aacgctggat caagtgaaag aggccgtggt
     1801 gaagattgag attcccctgg agggtggggc agctatcgag acggggaccg ggtttttcat
     1861 cgacaaacga ggttggatcg ccaccaacta tcacctggtt cagaacatga ataacagggc
     1921 gtatgtccgg tcggcgggtg gtcaaacgta tcggattgcc ggaatcgtgg cggaagcccc
     1981 ggctatggat ttggccatcc tcgaactggc ggagcgaccg gtgaggatga tgatcctcga
     2041 tctttcttat aacgacaccc cgcgcctggg gacggaagtc tatgccttcg ggcatcccta
     2101 caatgtggat ttttcgctgt cacgaggggt cgtcagtcgt gttcttacga cctcggaatt
     2161 gatccagaat ttccctgatc acatcgtgac gaaaatcaaa tcacctccgg ccatgatttg
     2221 gatccaacat gatgcccgga tttcccccgg caatagtggg ggcccgcttc tcgacggcac
     2281 cggtcgtgtg attggcgtca acacctttgt gcataagctg gctcaatatg ggtttgcgag
     2341 ccatatccgc tatttgcgag agttggccca gggggcctcg ggagttgttc gttctttgcc
     2401 ggagcccgcc ccgccacgtc tggaggggat cgcctcggag tctgttgtcg atctgcgtca
     2461 gatttcaccg ctttggcagt ttgcgcagtc gattatgtgg acgccccaga gcttggaaca
     2521 gtaccaaaaa ctggcggagt gggcacggat cgccaatatc gtcaagctcc tacaaattca
     2581 agggaagatt cctgaaggtg tcccgcccgc agtgctcgat caggcggcag tcgaggcgga
     2641 aaacctcttc aagacattga caccggacca catttcccaa gaagcacggg atcgtctcaa
     2701 tgagctggct acgcaaatcg gttttcaacc gcttaacggg attgtcttcg tagcacagct
     2761 catcgcagag ccatcgcctg gcatggtttt attaacgttt ggcagcgaaa accagcatgc
     2821 tcttgttcgg cttcggcccg atcgccccga tgtgcgaatc ggtgccgaag tgttcgtggc
     2881 gggcttggtt tcgccgcagg ctgccgagat tcgcattggc caagaagggc ggcaagagcg
     2941 ggtgcccctt atttggggaa tgttcctgct aaatctcgaa aaaccttccc aaactgcgtc
     3001 tgccgaagcg gcacgctaac tttttccaag aagtcggtcc acggccaggc cgatgtcggc
     3061 ttcccgcatg aggctttccc cgaccaagat ggcgttgacc cccgcccggc gaaggcgatc
     3121 cacatcctca tgggtttgga tgccactctc gcccaccacc agccgatccg aaggaatttt
     3181 cgcccgcaag cggatcgtgt gttcgagatc gacctcgaac gtctgcaaat tacggttgtt
     3241 aatcccgata agcgaagccc ccaggtcaat cactcgcggc aggttttccg gctcataaac
     3301 ctcgaccagt ggtgtcatgc cccaggcgag aatctcatgg taaagacgtg tcaactgctc
     3361 ggtagtcaag cattcagcaa tgagcagaac cgcatcggcg ccgtaagcgc gggcttcgag
     3421 aacctggtaa gagtcgagga taaaatcttt ccgcagcacc ggaatctgca cgctttcctt
     3481 gacccaacga aggaattcga tgtgcccctg aaaatacggc tcatccgtca aaacactgat
     3541 gcaggtggca ccgtgctttt cgtagattcg ggcgatcgcg acggggtcaa agttctcacg
     3601 gatgacaccc ttggatggac tggccttttt gacttccgca attaaggcga tgttgggtgc
     3661 attgcggatt gcccccagaa aatcccgcgg tggtggctgt tgttccacca tgcgacggag
     3721 atgctcctcg ggcgcaaaaa tctttgcccg ctccacttcc tcgcgcttgg tggccacaat
     3781 acgatcgaga atggaagcca tgtcgagata acctccaact tcacggttta ttggggcatc
     3841 cccctcgtgc atgcccgcgc acaacctgtt gactgcacac ggtcttccca ccaccgcggc
     3901 aatatcgccc gttgggccat cagtcccgga gcagcgattg tcaaatctgt gacacgggac
     3961 ttggccggag cacaatgttc gtgatgttgg ataccgtttg cgcgtactcc ggcttatcgc
     4021 gcaactcaag ccactgcgga tctttcagaa atgctgccca ggcttctttt tgtgcggcct
     4081 cgttgtcaaa acccaacatg taggtcagat tgggaagtct ggacccgatc aacgcttgcc
     4141 caaagaagac cggattcatt ttgcaccgat agaaaatttc caattcccca ccggtgttaa
     4201 acatctcaac tttcttcaca tgcttcgtca gattgtggct ctcgtaaatg cggagctgaa
     4261 aaacccgcga ttccgccgtg gtaggaactt ggaccttggg aacgccatcg aaggcaagca
     4321 agaggctact ctcgacccgc tgataggtgg gatcgtcgaa cgggtcgtac aggacatctt
     4381 tgccgactga ctgggccttg ggatcggcca acagggccgc catctctcga atcaccgtct
     4441 cggcgctgtt atgtggcagc aagacaaaaa gacttggatc ttcttctccc aaatagcgaa
     4501 acacgccaac gggttgaatt cccaagctgt tccagacggg aatggcaacc tcggcaaaga
     4561 atttctccaa acggtctcgt ttttctggag atgcgatctg ataacgacgc aactcgagta
     4621 attgctttgg cccaccggcc ccttcaacgg cctgggataa ctcactcatg gccatgatgg
     4681 ctccgactcc cccaatcatg gacgacgcca ggaactctcg ccgttgcatg gtgtaactcc
     4741 tcgttcaaaa gtgacctatc aagatgccaa acgccttaag aagcacgcga cgtcttctta
     4801 gagcgtatcc catagtctcc cctcgcccgc tcggtgggag aggggtcggg ggtgagggtg
     4861 gctcgaatcg ctgccggttt tctcgttcga ccccggcgat cgcttgccct caccccaacc
     4921 ctctcccaga cggagagggg gagctttttg ggacagactc ttagtgtaac gactcaccgt
     4981 gggcaagtgt acccaaagcg atatgaccag cgtttcggat tggaccgaac cgctatttgg
     5041 cggaggcgac tagcaagaaa agccgagagc atatcccata atctcccctc gcccgctcgg
//
LOCUS       DAOSUT010000232         5078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7275_length_5078_cov_9.782401, whole genome shotgun sequence.
ACCESSION   DAOSUT010000232 DAOSUT010000000
VERSION     DAOSUT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7275_length_5078_cov_9.782401"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLD05_14750"
     CDS             complement(<1..1358)
                     /locus_tag="PLD05_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78745.1"
                     /translation="MRNWSLPVLLTLLLFVRPHLALSDLPAIESVTTTPDRVIVVNGR
                     PFLPIMAWLQPPDNFAILKKCGINTTAGYWPGSGGTKDVADYMTRVREAGLYGVMPYH
                     PALHNHPNLLAYIHDDEPDLPRQVSDAKVTPGEGLRINARTPLWRMVDGDESSWSVLD
                     PLENATWTVTLNQPETVQRVAIWVTVSPGLSLPEEVVLSAEDKEVGRFSLEPKRGKQE
                     FALPSPVTAQEWRFTVTKVKPGEQVWGSISEIAAYTAEGKNVFLSPPRWVPRAMPEET
                     LKVYRAVKAADPSRRMFMTFTGYFHPHFGKWSDEQRQNLYPAYIQATDVVGFDIYPIY
                     GWNKPEWIHLVYEATEQLTALGGHRPVYAWIETSKGGQYTGDLERQKDVRPEHIRAEV
                     WMALCGGATAIGYFTHIWKPSYRQFGVPEENQVALRAINDQLTRLAPAILSKRNTREL
                     RL"
     gene            1542..4064
                     /locus_tag="PLD05_14755"
     CDS             1542..4064
                     /locus_tag="PLD05_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOP78746.1"
                     /translation="MASLGDNYQDIFQAELAAGGREDYIDDFANVRVCPLLVVTSVVF
                     ARPNGGFEMGIRRSPANLMIGNKWLSGFAVHGVPRLVWQMLVLVFLAAAVLGGYGSSQ
                     AWAAEPGVDATTTLKVQLSTMDLIIVFGYLVGIIVLGCLAGTRRRQAKGTDYFLASKS
                     LTWPMIGMALFATNISTIHLVSFAQNGFTSGLTYGNYEWMATFTLVILSLFFAPFYLR
                     ANVATLPDFLERRYSRACRDYLAILSIFSAVVVHIGFSLLTGAIVLEGTVLPAFVNGS
                     PEEYRFLTVLAMCGATAIYTIVGGLLAVVWTETIQTVVLLVGAACITFIGMRMIGGWE
                     ALQQSVHPVNLSILRSSADPTGVTWYAVFLGYPVLGVWYWCTDQTIVQRVLGAKDESH
                     ARIGPLFAGFLKILPVFLFVLPGVICLGLVNTGKLPPLPTTADGLPDTQKTYTHMINT
                     MLSPGFRGIVVAALLAALMSTVSGALNSIATLFSYDIYKRWVGEVSDRHLVWVGRIAT
                     FVAMCIAIAWTMALGRRDTTIFQAMVDVFPVVAPPTAVVFLWGVFWRGTSGRAAFITL
                     VGGSLIGLLVFYLTVIKVITINSLFMAFILFVVESVGIFLLSLIFPHHHTAESESLVW
                     KRPWDALRAENAWRGLMDFRVFAALLVITMVALYWWFTSPHVYYPIRAKILVDGQPAP
                     GVEVTLDADDDRLDDVLVADADGSVTYGTVMRAGGAPEGTVFRVAVRPARDYIVELRP
                     RKNGEGIHNEDVLILAALPHGTEVAEELAGEYKVYRWEAEGQKKEIRVPASAKVEIRR
                     SPDIPARYRDPSQSGITVVALPEVQVQEVNIFTREVALAERP"
     gene            4180..4395
                     /locus_tag="PLD05_14760"
     CDS             4180..4395
                     /locus_tag="PLD05_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78747.1"
                     /translation="MVQIDLSTEEWQLILELLERERADLHCEVRHARFYDVRELLRQK
                     REAVEKLIERIESHLGGSVEVAAPEAG"
     gene            complement(4491..>5078)
                     /locus_tag="PLD05_14765"
     CDS             complement(4491..>5078)
                     /locus_tag="PLD05_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOP78748.1"
                     /translation="HKFMLCFVPLFAAVDAAGLLPLYLALTDGLSPTVKFRVLRQMLL
                     TAILVVVAFVFIGRMILDFLGVTISDFMVAGGTMLFIVAMGDLFVVDKGRPHVDTESL
                     GAVPLGVPLTVGPAVLTTVLLLANAYGRLPALTAAVVNVVLAAIAFSWAANIEKLLGR
                     NGVRALSKIASLFIGAIAVMMVRKGVFEMIASLQN"
BASE COUNT         1066 a         1323 c         1468 g         1221 t
ORIGIN      
        1 cttagtcgaa gttctcttgt gtttcgcttg ctcagaatag cgggtgccag acgggtcagt
       61 tggtcattga tggcccgaag tgccacttgg ttttcttccg ggacgccaaa ttgccgatag
      121 gatggcttcc aaatatgggt gaaatagccg atggctgtcg cgccaccgca aagggccatc
      181 catacttccg cgcgaatgtg ttcgggtcgc acatctttct gtcgttcgag gtcccccgtg
      241 tattgcccgc ctttactcgt ctcaatccag gcataaactg ggcgatgacc ccccaaggct
      301 gtgagttgtt ctgttgcttc atagaccaaa tggatccact ctggcttgtt ccatccgtag
      361 atgggataga tgtcaaaccc cacgacgtcc gtggcctgga tgtaagcagg atacagattc
      421 tgtcgttgtt cgtcgctcca tttgccaaaa tggggatgaa agtagccggt gaacgtcatg
      481 aacatacgcc gcgagggatc agcagccttg acggcgcgat agaccttcag cgtctcctca
      541 ggcatcgccc ggggaaccca acgtggcggg ctgaggaaga cgtttttgcc ttcggccgtg
      601 taggccgcaa tctcactgat cgaaccccaa acctgctcgc caggcttaac tttggtcacc
      661 gtaaaacgcc attcctgggc ggttaccggg gaaggaagcg caaattcctg cttaccccgt
      721 tttggctcaa gggagaatcg ccctacctct ttgtcctcgg cggaaagaac aacttcttcg
      781 ggcaaactca ggcccggtga aaccgtgacc cagatggcca cacgttgcac cgtctctggc
      841 tggttgagtg tgactgtcca tgtggcgttc tccaggggat ccaagaccga ccaggaactt
      901 tcgtccccat caaccattct ccacaaaggt gtgcgagcgt tgatccggag gccttccccc
      961 ggggtgacct tcgcatcgct cacctgtcgc gggagatcgg gctcgtcatc gtggatataa
     1021 gctaaaagat tcggatggtt gtgaagtgcc ggatgataag gcatcacgcc gtagaggccg
     1081 gcttctcgaa ctctcgtcat ataatccgcg acgtcttttg ttccaccgga ccccggccag
     1141 taaccggctg tcgtgttgat tccacatttt ttcagaattg cgaaattatc cggcggctgg
     1201 agccaggcca tgatgggtag aaatgggcgt ccgttcacga cgatgacgcg atccggggtg
     1261 gtggtaacag actcaatggc gggaaggtcg ctcaaggcca gatgaggacg aacgaacaat
     1321 aacaacgtta gcagaaccgg cagcgaccaa tttctcatac ggcattctcc ttatggaagt
     1381 gcaatccgac tcattgattg gcacatctga tgtttggggt aagcttagcc cacggcgaac
     1441 ctctcgtcac gatgtcaaag ctctcataac ttggatctga gcatgtaacg acgagaattg
     1501 taccctgcgg cgtgccgatt cggaacacga ggacgacaac cttggcaagc ctcggggaca
     1561 actaccagga catctttcag gccgagcttg ccgctggtgg acgtgaggat tacattgacg
     1621 attttgccaa cgttcgtgtt tgcccgttat tggtggttac atccgttgta tttgcacgac
     1681 ccaacggagg gttcgagatg ggaataagac ggtcaccggc aaatcttatg attgggaaca
     1741 agtggttgtc tggatttgct gtgcacgggg ttccgcgcct ggtgtggcaa atgctcgtac
     1801 tggttttctt ggccgccgcg gtgttagggg ggtacggttc ttcacaagcc tgggcggcag
     1861 agccgggagt cgatgccaca acgactctga aggtgcaatt gtcaacaatg gatttgatca
     1921 ttgtgttcgg gtatctcgtt ggcattattg tgttgggatg tctggctggc actcggcgtc
     1981 gtcaagcgaa ggggaccgat tactttctag caagcaaatc ccttacctgg cccatgattg
     2041 ggatggcact ttttgccacg aacatctcca cgattcacct ggtgagtttt gcccagaacg
     2101 gtttcacatc ggggcttacc tatggcaact atgagtggat ggcaacgttc acgttggtta
     2161 tcctttccct cttttttgcc ccgttttact tgagggcgaa tgtggcaacg ttgccagact
     2221 ttctggaacg ccgttacagt cgtgcctgtc gagactatct cgctattttg tccatattct
     2281 cagccgtcgt tgttcacatc ggattttcat tgttgacggg agccattgtg ttggagggga
     2341 cggttcttcc cgcatttgtc aacggaagtc cagaggaata tcggttcctg accgttctcg
     2401 ccatgtgtgg tgccacggcc atttacacca ttgtcggggg actcttggct gtggtctgga
     2461 ccgagacgat ccagacggtt gtccttctcg tcggagcggc ctgtatcacg ttcattggga
     2521 tgcggatgat cgggggctgg gaagcgctgc agcagagtgt tcatcccgta aatctaagta
     2581 ttcttcgatc cagcgcggat ccaacgggtg taacatggta tgccgtgttt ttgggatacc
     2641 ccgtgttggg ggtgtggtat tggtgcacag atcagaccat cgtgcaacgg gtgctcgggg
     2701 ccaaggacga gagtcacgcc cgaatcggcc cactgttcgc tggtttcctc aagattttgc
     2761 ctgtgtttct ctttgtatta ccaggtgtga tttgcctggg gctcgtaaac acgggtaagc
     2821 ttcccccgct gccgacaacc gccgatggcc tgcccgatac ccaaaaaacc tatacacaca
     2881 tgatcaacac gatgctcagc cctggttttc ggggaattgt ggtggccgcc ctgttggcgg
     2941 ctttgatgag tacggtttcg ggcgcactga actccattgc gacgttattt agctatgaca
     3001 tctacaagcg atgggtgggc gaggtgagcg atcgccatct ggtttgggtg ggacggatcg
     3061 ccacctttgt cgctatgtgt attgccattg cgtggacaat ggcgttagga cgccgcgaca
     3121 cgaccatctt tcaggccatg gtcgatgtct tccccgtggt ggcacctccc actgccgttg
     3181 tcttcctttg gggggtattt tggcggggga catcgggccg tgcggcgttt atcacacttg
     3241 tgggcggttc gttgattggc ctcctcgtgt tctatcttac cgtgatcaag gtcatcacga
     3301 tcaatagtct tttcatggcg ttcatccttt ttgtggtgga atcggttgga atctttctcc
     3361 tctcgctgat cttcccccac catcacacgg cggagagcga aagccttgta tggaagagac
     3421 cctgggacgc tttgcgggct gagaatgcct ggcgtggcct gatggatttt cgcgtctttg
     3481 cggccttgct ggtgatcacc atggtggccc tctattggtg gttcacaagt ccccatgtct
     3541 attatcccat tcgggcgaag atccttgtcg atggtcaacc cgctcccggg gttgaagtca
     3601 cactggatgc tgatgacgat cgcttagacg acgtgctggt ggctgatgct gacgggagcg
     3661 tcacctacgg gacggtcatg cgagccggag gagcaccgga aggaacggtc ttccgagtcg
     3721 ctgtccgacc cgccagagat tacatcgttg aattgagacc aaggaaaaat ggggagggga
     3781 tacacaacga ggacgttcta atcttggctg cattgcccca cggaacggag gtcgcagaag
     3841 agttggcggg agaatataag gtttatcgat gggaagcgga ggggcaaaag aaggaaattc
     3901 gcgtccctgc ctcagccaaa gtagaaatcc gtcgatcgcc cgatatcccg gcgcgatatc
     3961 gtgatcccag ccaatcgggg ataaccgtgg tcgcgttgcc cgaggtccaa gtccaggagg
     4021 ttaatatctt cacgcgggaa gtcgccctcg cagaacgacc gtaaatctcc ctatcagaga
     4081 ggcgtgttcc ggcgagttgc tccccttgtc ggaggcatgt cccgttttta cgattctcgt
     4141 ggggctgatt tggcttttcc tggtacggga ggtgcggtta tggttcagat cgatctctcc
     4201 accgaagaat ggcaactgat cttggagctt ctggagcggg aacgcgcaga cctccattgc
     4261 gaggtccggc acgctcggtt ctacgacgtc cgtgagctgc tccgccaaaa gcgcgaagcc
     4321 gtcgagaagt taattgagag gatcgaatcg catcttggcg gttccgttga ggtggcggcc
     4381 cctgaggcgg gttgactccg ttagtctttc caacgtgggc cgcgacaaag ccgggtaatg
     4441 ctctttttgt aattgccccc actaactttt gatcggaggg ccgggtgtct ctaattctgc
     4501 agcgaggcaa tcatttcgaa gacacctttg cggaccatca tcacggcaat ggccccaatg
     4561 aacagggagg ctatcttgga caatgccctg accccgtttc gacctaaaag tttctcgatg
     4621 ttggcggccc aagagaacgc gatggcggca agaaccacgt tgacgacagc ggccgttaag
     4681 gcaggaagcc gtccataggc attggcaagg agaaggaccg tggtcagcac ggcaggccca
     4741 acggtgaggg ggacaccaag cggtacggcc cctagcgatt cggtatctac atgcggccgc
     4801 cccttatcga cgacgaacag atcgcccatc gccacaatga aaagcatcgt tccaccggcc
     4861 accataaaat ccgagatcgt gacacccagg aaatcgagaa tcatccgtcc aataaagacg
     4921 aatgccacaa caacgaggat tgcagtcaag agcatttgtc ggagaacgcg gaacttgact
     4981 gtgggcgaca gaccatcggt aagggccaga taaagcggca acagccctgc tgcatcgaca
     5041 gccgcaaaaa ggggaacaaa acagagcatg aatttgtg
//
LOCUS       DAOSUT010000233         4999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7386_length_4999_cov_8.647451, whole genome shotgun sequence.
ACCESSION   DAOSUT010000233 DAOSUT010000000
VERSION     DAOSUT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7386_length_4999_cov_8.647451"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLD05_14770"
     CDS             complement(<1..386)
                     /locus_tag="PLD05_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78749.1"
                     /translation="MRRDLARWWLAVTVLAVSVGITPVFVRGAESQKAPPNILFIAID
                     DLNDWTGFLGGHPQAKTPNLDKLAQRGMIFTRAYCSAPACNPSRASLLTGVLPSTSGV
                     YHNDNPWRPQMPDTITLFQHFMANGY"
     gene            716..1561
                     /locus_tag="PLD05_14775"
     CDS             716..1561
                     /locus_tag="PLD05_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP78750.1"
                     /translation="MEKWPIGVFASIDAGLGVQLSVAHELGVPTIQLHTPKKSSRTPE
                     NAKRFLQQLQEYGLTITVVFAGFEGESYADIPTVAKTVGLVPPETRKERKQELKEIAD
                     FAKLLGVDAVGLHIGFIPHDREDPLYQDVLKVAQEVCDYCANQGQRIHLETGQESADT
                     LLQFLKDVNRSNLFVNFDPANMILYGSGDPIEALKKVGAYVRSVHCKDAKWAAQAGKE
                     WGVEVPFGTGDVNAELFLKTLAELGYDGALTIEREIPQEPERQKQEIGHAIRVIQEIK
                     RKIFG"
     gene            1605..2249
                     /locus_tag="PLD05_14780"
     CDS             1605..2249
                     /locus_tag="PLD05_14780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HOP78751.1"
                     /translation="MITRICGVLVQITEETATLEAGSFWWEVLIPEYTRRQLQSRINQ
                     EVVLHTTLYLEGAVNTGRLTPRLVGFINEIEREFFDLLCTVDGMGAKKALRAMSHPVN
                     EIANAIEQQDHRFLASLPGVGPATAERIVAKLRRKMPKFALWVTQQKVAEEALVEPDT
                     VTQTFQTLVALGHSEAEARRLIDAVLARKKKFASVDEMLHAIYERQTQQGASQS"
     gene            2246..3331
                     /locus_tag="PLD05_14785"
     CDS             2246..3331
                     /locus_tag="PLD05_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOP78752.1"
                     /translation="MTQLLSTGKHIQTRVKDFSYAPSVQTEACSRCGAPVESHDKYCP
                     ACGAAQVVEAEVLTPRAIPERVSCDNCGAEIRLAPNQRSYTCEYCGSTYVFDFAPEVT
                     GRQPPEFVLGFAITPENAQKILLDWVRQKAQWISRGVREAIQQAVIRGVYLPFWSFSM
                     LAESRWQATIGEYWYRTETYTTYENGKLVTKTRRVRETEWWPLAGKYHQYLSGYLVSA
                     SQSVPQSFADQLMPYYLAGLRRYDPSYLAGWACEEYTLERPQAESLCKEYFRKYQTEC
                     ISAFLPGDTYRGLVVETDFSKESSDLILLPVYNIRFKYRGKEYRLWMNGQTGKIVGQL
                     PQSWVIPVVVIIAILGLAAIGAYLLLR"
     gene            complement(3422..3799)
                     /locus_tag="PLD05_14790"
     CDS             complement(3422..3799)
                     /locus_tag="PLD05_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOP78753.1"
                     /translation="MTTLSLGWDLDVTQGEGWLIIRPRPHGPQTLELVPLADEILKLL
                     EWCWANRVILVLDGLDLLTSFVVGQFVKLHKNLERQGGILRLCGVKPQHRQVLERVGL
                     TMCLPIYDDVRDAVMGSFPCKPR"
     gene            complement(4053..4799)
                     /locus_tag="PLD05_14795"
     CDS             complement(4053..4799)
                     /locus_tag="PLD05_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOP78754.1"
                     /translation="MAQTVPGITDIGFQSLGQQYIRIAEVFQSRQGEGLLTGTESVFV
                     RTSGCNLRCWFCDTPYASWAIEGDRLTIDEIISRVTSFSARHVVITGGEPMLFPGVVD
                     LTHRLESKGYHITIETSGTRYLPVACHLLSISPKLSNSTPSWKEHPRWAAAHERRRYC
                     PSVVRELIRRYPYQIKFVIDTERDVAEVENYLEEVPEIDRHRVLLMPQGIDQRVLEEK
                     ARWLKPLCEKHGFLFCPRKQIEWYGPVRGT"
     gene            complement(4817..>4999)
                     /locus_tag="PLD05_14800"
     CDS             complement(4817..>4999)
                     /locus_tag="PLD05_14800"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOP78755.1"
                     /translation="RLSKAEIIHRGLALGMNYGLTHTCYDPTPDGRACGRCDACQLRK
                     KGFLEAGVPDPIPYAE"
BASE COUNT         1156 a         1411 c         1381 g         1051 t
ORIGIN      
        1 ttatacccgt tggccatgaa atgctgaaag agcgtgattg tgtccggcat ctgaggccgc
       61 caggggttgt cgttgtgata gacgcccgag gtcgaaggca acactcccgt aagaaggctt
      121 gcccgagacg gattgcatgc cggggccgaa caataggccc gcgtgaaaat catcccccgc
      181 tgggccaact tgtccaagtt tggggtcttg gcttgcggat ggccccccag aaatcccgtc
      241 cagtcattca gatcgtcaat cgcaataaaa aggatattgg gcggtgcctt ctgcgattcc
      301 gcgccccgaa cgaaaaccgg cgttattccc accgagaccg ctaacacagt cacggccagc
      361 caccatcgtg caagatcgcg tctcatggaa aagactcctg tggttcttag cttgagctga
      421 tcgacccgtc tggacccatg ccgagaaaaa ctcacccttc tcaaagctcc ccgcaactcg
      481 agaccgcttg cccatactca tgaattgcca aaacttgcca gcgcgcacca agggcctacg
      541 atgcgacccc tccggtgggg tcggccgttg cttgcagttg gtgggcttct gcggtatctt
      601 gactacaacg cttgttttac gcgggaatcg tttggggaag tccagggcaa cgcggagccg
      661 tcatcggccg cgactgtcgc tggccaaggg tcgaaaggtc gtggaggact cagctgtgga
      721 aaaatggcca atcggtgttt ttgccagtat tgacgcagga ttaggcgtgc aattgagcgt
      781 tgctcacgaa ttgggggtac ccaccatcca gttgcacacg ccgaagaaaa gttctcggac
      841 acctgagaat gccaaacgct ttttgcaaca attgcaagag tacggactca ccattacggt
      901 cgttttcgcc ggtttcgagg gcgaaagcta cgcggatatt ccaacggtgg caaagaccgt
      961 cggccttgtc ccgccggaaa cccgcaagga gcgcaagcag gaactcaaag aaatcgcgga
     1021 cttcgcaaag cttcttggcg tggatgcggt gggtctccat atcgggttca ttccccacga
     1081 ccgagaggat ccgctttatc aggatgtttt gaaagttgcc caggaggtct gtgattactg
     1141 tgccaaccaa gggcagcgca tccatctaga gacgggccaa gaatccgccg atacgttgct
     1201 gcaattccta aaggatgtga atcggagcaa cctgttcgtc aactttgatc ctgccaacat
     1261 gatcctttac gggtccggtg atccgatcga ggccctcaaa aaggtcgggg cttacgtgcg
     1321 aagtgtccac tgcaaggatg ccaaatgggc tgcccaagcc ggtaaagagt ggggagtcga
     1381 agtgcctttc ggcaccgggg atgtaaatgc cgagctgttc ttgaagacct tggccgaact
     1441 gggttacgac ggtgccttaa ccattgaacg ggagattccg caggaaccag aacggcaaaa
     1501 acaggagatc ggccacgcta ttcgcgtcat tcaagagatc aaacggaaga ttttcggtta
     1561 acggacgatc gaatccttga gttattagga aggacgttcc acccttgatc acgcgcattt
     1621 gcggtgtatt ggttcaaatc acggaggaaa ccgcaacact cgaagctggt tcgttctggt
     1681 gggaggtttt aatccccgaa tacacacggc gacaacttca atcgcgcatc aatcaggagg
     1741 ttgtcttaca tacgaccctc tatctggagg gtgcggtcaa cacgggtcga ttaacgccgc
     1801 ggttggtagg ttttatcaac gagatcgagc gcgagttttt tgacttgcta tgcaccgtcg
     1861 atggcatggg ggctaagaag gccctgcgtg ccatgtcgca ccctgtcaat gaaatcgcca
     1921 acgcgatcga gcagcaggat caccgctttc ttgcgagcct gccgggagtg gggccggcca
     1981 cagcagagcg gattgtggcc aagttgcggc ggaagatgcc caagtttgcc ctatgggtaa
     2041 ctcagcaaaa ggttgccgaa gaggccctcg tcgagcccga caccgtgacc cagacctttc
     2101 agaccttggt ggcccttggt cacagcgagg ccgaggcccg tcgcctcatt gatgccgtgt
     2161 tggcccgcaa gaagaagttc gccagtgtcg atgagatgct gcacgcaatc tacgaacggc
     2221 aaacccaaca gggggcgtcg caatcgtgac acagctcctt tccaccggaa aacatatcca
     2281 aacgcgcgtc aaagatttca gttatgcccc gagtgtccaa acggaggcat gttcccgttg
     2341 tggggcccca gtggagtccc acgacaaata ttgccccgct tgtggtgctg cacaagttgt
     2401 cgaggcagag gtgctcacac cacgggcaat ccccgaacgc gtgagctgcg acaattgcgg
     2461 tgccgagatt cggttagctc ccaatcaacg cagctacacc tgtgagtatt gcggcagtac
     2521 gtatgtcttc gattttgccc cggaggtcac cggccgacaa ccgcccgagt ttgtgctggg
     2581 ttttgccatc accccggaga atgcccagaa gatcctcctt gactgggtcc gccaaaaggc
     2641 acagtggatt tctcgcgggg tgcgcgaagc tattcagcag gccgttatcc gtggcgtgta
     2701 tcttcccttc tggtcctttt ctatgctagc ggaaagtcgt tggcaggcca cgatcggaga
     2761 gtactggtac cgcacggaaa cttacacgac atacgaaaat ggaaaattgg tgacaaagac
     2821 gcggcgggtc cgggagaccg aatggtggcc cttagcgggc aagtaccacc aatacttgag
     2881 cggttatctc gtctcggcca gtcaaagtgt cccccaaagt tttgccgatc aacttatgcc
     2941 ctattatttg gcagggttgc gacgatacga cccgtcgtac ttggcaggtt gggcgtgcga
     3001 ggaatacacc ctggaacggc cccaggcaga gagcctttgc aaggaatatt ttcgaaaata
     3061 ccagacagag tgcattagtg cgttccttcc aggcgacacc taccgcggtc tggttgtgga
     3121 aacggatttc tccaaggaaa gctccgacct tatccttctg cccgtctata acatccgctt
     3181 caagtatcgg ggaaaagagt accgcctatg gatgaacggc cagacgggca aaatcgtcgg
     3241 ccaactccca caatcgtggg tcatccccgt ggtcgtgatc attgccatct tgggcttagc
     3301 cgcgattggg gcttatcttc ttttgcgcta aactcgctcc ccttccctgg caatgacaga
     3361 gactaacgtc ccggagcgca aacttcccac cgtctgttac ccgcctcggg atcaatgcga
     3421 actaccgggg tttgcagggg aaactcccca tcacggcgtc tcgcacgtcg tcatagatcg
     3481 gcaaacacat ggtcagtccg acgcgctcca aaacttggcg atgttgaggt ttgaccccgc
     3541 aaagccgcag aatacccccc tgcctctcaa gatttttgtg gagcttcaca aattggccga
     3601 ccacaaacga ggtcaacaaa tcgaggccgt caagaaccaa gatcacgcga tttgcccaac
     3661 accactccaa cagtttgagg atttcatcag ccaaagggac cagttccagc gtctgtggtc
     3721 cgtgtggccg cggccgaatg atcaaccaac cttccccctg ggtcacatcg aggtcccaac
     3781 cgagcgagag agtcgtcatg gatcgactcc tttcttcccc cgagccacgt ggtgacgagt
     3841 gagagatccc agcacacaag gtgcaaaacc gcgatgccaa cggcttattc taccccaagg
     3901 cgcagccgtg ccaatccccc atcctggccg tattaactga cattgaagtc gggcatttac
     3961 ctccggctcc gatatgattg cggtgctatc ggtgctgtca catcaggcgc atcggccgat
     4021 ggcaggcacc aggagaattg cgaacgacaa tgtcatgtcc cgcggaccgg cccataccac
     4081 tcgatttgtt ttcgcggaca aaataaaaac ccatgtttct cgcaaagcgg cttcagccaa
     4141 cgcgccttct cttctaagac tcgctggtcg atcccctggg gcatcaggag gacgcggtgg
     4201 cggtcgattt ccggcacttc ctccaggtaa ttctcgacct cggccacatc gcgttccgtg
     4261 tcgatgacaa atttgatctg gtaagggtaa cggcggataa gttcccggac aaccgatggg
     4321 cagtagcggc ggcgctcgtg ggcagcggcc cagcggggat gctccttcca ggagggcgtc
     4381 gaattgctga gcttggggct gatcgagagg agatgacagg caaccggcag ataccgcgtc
     4441 cccgaagtct caatcgtaat gtggtagccc ttactctcga gacgatgtgt caaatcgacg
     4501 acacctggga aaagcatcgg ctcgccaccg gtaatgacaa cgtggcgcgc cgaaaaactg
     4561 gtcacgcggg agatgatctc atcgatggtc agacgatcgc cttcaatggc ccaactggcg
     4621 tagggcgtat cgcaaaacca acagcgaagg ttacacccag aggtgcggac aaacacactc
     4681 tctgtacccg tcaaaagccc ttccccctgc cgagattgga aaacctcggc aatgcggatg
     4741 tactgttgtc ccaaagattg gaatcctata tcggtaatgc caggcactgt ttgagccatg
     4801 tttcaaccaa aaatcgtcac tcggcataag ggatgggatc cgggacgccg gcttcgagaa
     4861 atcccttttt tcgcagctga caggcatcac accgaccgca tgcccgtccg tcgggcgttg
     4921 gatcgtagca ggtgtgggtt aacccgtaat tcattcccaa ggccaacccc cgatggataa
     4981 tctccgcctt acttagccg
//
LOCUS       DAOSUT010000234         4978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7419_length_4978_cov_9.207191, whole genome shotgun sequence.
ACCESSION   DAOSUT010000234 DAOSUT010000000
VERSION     DAOSUT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_4978_cov_9.207191"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLD05_14805"
     CDS             complement(<1..1307)
                     /locus_tag="PLD05_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOP78756.1"
                     /translation="MSHQALDRVPEGRPSASRVETPLPTATAIEIAKSWFPGDDFACD
                     AHSESLVFSVQIYPTGWNRAIQRGCESVVQSVDITCKPPRVLSLQIRDPVICVRSFFF
                     LADEQTEGSSSQQRCQNVSGSNAAQSRQQESQNDPQTPGRSRLRCCAEELLRLVTAGH
                     QHRIEVPEDVIKLEDRLRYLLQPSLEAILAERQLEFPFTPFPYQLEGVAFLFPRHAAI
                     LADEMGLGKTMQAITAIRLLLRCGEVRRVLLVCPKPLVSNWRREFELWAPEIPVMVIE
                     GDPQRRKWQWELADVPVRIANYELLPRDQEFFTASDETGQTKVAFDLVVLDESQRIKN
                     RHSATNQAVCSIARKRSWALTGTPVENSTDDLVGIFEFLSPGFLSPDMKPQRLGRTIA
                     DYVLRRTKDRVLSDLPPKILRDAEVELTPEQRETYDLAETAGVL"
     gene            complement(1372..2007)
                     /locus_tag="PLD05_14810"
     CDS             complement(1372..2007)
                     /locus_tag="PLD05_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78757.1"
                     /translation="MREHHDSVGNRLALRLGFFSVCPHVDGGFIGGYLILNGVGRPLE
                     FHCTTPVKPTRTQEILFGATLKPYLFGEQIGPVLFAKAKTPVHAIFVSQWESLTLASQ
                     ISEPVAFVCPEGESPLLEGADGASDVPRSNGRWETSPSNETQTCGVIERVGLPPCQVV
                     RIGKNRLVVAAIRPEAPEELRELLSPWEAGLDLLEPFGRIQAALEEASRRG"
     gene            2282..2767
                     /locus_tag="PLD05_14815"
     CDS             2282..2767
                     /locus_tag="PLD05_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78758.1"
                     /translation="MMSENRPVRPPLFTQIDVSQATPGPTPAPAGSLAEVAMLLRQLL
                     AIEQRQTKLLEDLLNQVSITQRQRAAELGQWRQANPHLAKKCREAAEALARVQTEFLH
                     QLTEEVNNNFDALLDGDFMFTEFVDRFGPRMAHLNGILQVLAQLSSPPAPTNSSNNNS
                     P"
     gene            3230..4393
                     /locus_tag="PLD05_14820"
     CDS             3230..4393
                     /locus_tag="PLD05_14820"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOP78759.1"
                     /translation="MAKQCWVITDVARGIALDELTLTSGVTSAVPKGVSITKRTLHGG
                     LQDGVDVVDVDNGRLRFTVIPTRGMGVWRAWCDNIQLGWQAPVHGPVHPKWVNLWEPS
                     GLGWLDGFDELLVRCGLESNGAPEFDDKGILRYPLHGKIANRPACRVEVSMDSETGNI
                     TVRGEVYETRLFFTKLKLATEIRTTVGACSLTVTDTVTNVAAVPGEMELLYHINFGPP
                     VLEPGSKVTLPIQKLAPRDPIAAAEIDRWDQYGPESPGKPETAYFVQPLGDGRGQTLA
                     LLHNEAGTNGVRVDFNIKELPCFTLWKNEQPVCDGYVTGLEPGVNFPNRRSFEKAKGR
                     VVTLQPGEARRFTVEITALVTSDAVSTAREEILRLQTQAPPEVLRQPCLDWSP"
     gene            complement(4486..>4978)
                     /gene="smpB"
                     /locus_tag="PLD05_14825"
     CDS             complement(4486..>4978)
                     /gene="smpB"
                     /locus_tag="PLD05_14825"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOP78760.1"
                     /translation="AKSKKPVDAEHDNERVVTHNRRAKHQYDVLETLECGIALRGSEV
                     KSLRNGKVSIEEAFARVKDGEVWLYNCDIAEFPQANRFNHEPKRPRKLLLHRREIKRF
                     ASQASQKGLTLIPLKIYFKRGLAKVLLGLCRGRKEHDKREIIKKEESQREIRRMMQRY
                     QNR"
BASE COUNT         1083 a         1430 c         1371 g         1094 t
ORIGIN      
        1 aggacaccgg ccgtttccgc aaggtcgtag gtttccctct gctcgggagt caactcgacc
       61 tcggcatcgc gcagaatctt gggcggcaag tccgaaagaa cacgatcctt tgtacgacga
      121 agcacatagt cggcaatagt gcgtcctagc cgttggggct tcatgtcggg ggaaagaaag
      181 ccgggagaga gaaactcgaa aatccccacc aggtcatccg tgctattttc gaccggcgtt
      241 cccgtcaagg cccaactccg ctttctggcg atcgagcaca ccgcctgatt ggtcgcgcta
      301 tgacggttct tgatccgttg cgattcgtcc aggacgacga ggtcgaaggc cacctttgtc
      361 tggcccgttt catcactcgc tgtgaaaaac tcttggtccc tgggaagcaa ctcgtagttg
      421 gcgatcctca ccgggacgtc cgccaattcc cactgccact ttcgcctttg cggatcacct
      481 tcgatgacca tcacggggat ctcgggggcc cagagctcaa attctcgccg ccaattgctg
      541 actaagggct tgggacatac caggaggacg cgccgtacct cgccacaccg aagtaacaac
      601 cgaatggccg tgatggcctg catggtcttc ccgagaccca tctcatcggc cagaatcgcc
      661 gcatgccggg gaaaaagaaa agcaactcct tcaagttggt acggaaacgg cgtaaaggga
      721 aattccaatt gccgctcggc aagaatcgcc tccagcgaag gttgtagcaa ataacggagg
      781 cggtcctcca atttgatgac gtcttcgggc acttcgatgc ggtgctgatg ccccgcggta
      841 actagccgca atagttcttc ggcacagcac ctcaagcgtg aacggccggg tgtctgtgga
      901 tcattttgag attcctgctg acggctctgt gccgcatttg accccgaaac gttttgacac
      961 cgttgctgcg aggacgatcc ttcggtttgc tcgtcggcga gaaagaaaaa acttcgcacg
     1021 cagatcacag ggtcgcgaat ctgaagggag agaacacgtg ggggtttgca tgtgatatcg
     1081 accgattgaa ccactgattc acagccccgt tggatcgcgc gattccatcc cgtcggataa
     1141 atttgcactg aaaaaacgag tgactcagag tgggcatcgc aggcaaaatc atcaccgggg
     1201 aaccaactct tcgcaatttc gatggcggtc gctgttggca ggggcgtttc tactcgggat
     1261 gcgcttggcc gtccctcggg caccctgtca agcgcttggt gagacacgac agacgttgcc
     1321 ctcggttggc tctctgattg ccttcgcaaa gaccacggcc agaacatgcg atcacccccg
     1381 acgtgaagcc tcttcgagag ccgcttggat tcgtccaaat ggttcgagga gatcaagccc
     1441 cgcctcccaa gggctgagaa gttccctgag ctcctcaggc gcctcgggcc ggatagcagc
     1501 aaccacaaga cggttctttc caattcgcac cacttggcaa ggggggagcc ccactcgctc
     1561 gatcaccccg cacgtttgag tttcattgga gggagaggtc tcccatcgac cgttcgaccg
     1621 tggcacatcc gacgccccat ctgctccctc cagtaaaggt gattcgcctt cgggacagac
     1681 gaaggctact ggttccgaaa tctgtgacgc caaggtcaac gattcccact gcgagacgaa
     1741 aatcgcatgg acaggtgttt tggctttggc gaaaagcacc gggccaatct gctccccaaa
     1801 aaggtagggc ttcagtgtgg ccccaaagag gatttcttgg gttcgtgtcg gtttgacggg
     1861 cgtggtgcag tggaactcaa gcggtcgccc caccccgttg aggatgaggt acccaccgat
     1921 aaatccgcca tcgacatgcg gacagaccga aaaaaaaccc aatcgtagag ccaatcgatt
     1981 ccccaccgaa tcgtggtgtt cgcgcataag atctcaagag atgccagctc aaggttagca
     2041 aaacagatca attggggagc atggtccctc ctacggacat ggtctgcttc atcatcggaa
     2101 ccaaataagg cgcaacttta cggctttgcg gacattagca tgtagcggat ctataccgat
     2161 tgtgcggaac gaaccagaaa cttttcagtt atcggatgaa acttttgtta aagttctgtg
     2221 tcggacactc gactttgccc gcccgtgctg gttatactgt ttttgaattg gaggaggttt
     2281 catgatgagc gaaaacagac cagtgcggcc cccattattc acacaaattg acgtgagcca
     2341 agcgactcct ggaccgacac cagccccggc gggcagcttg gcagaggtgg ccatgctgct
     2401 tcggcagctt ttggcgattg agcagcgtca aacgaaattg ttggaggatc ttctcaatca
     2461 agtcagcatt acccagcgtc aacgggcggc tgaattgggg cagtggcggc aggcgaaccc
     2521 tcatctggcc aagaagtgcc gtgaagccgc cgaggcccta gcgcgggttc aaaccgagtt
     2581 tctccatcaa cttacggaag aagtcaacaa taactttgat gccctccttg atggtgactt
     2641 catgttcacc gagtttgtcg accggtttgg cccgcggatg gcccatctca acggtatcct
     2701 tcaggtgctt gctcaattga gcagccctcc ggcacctacc aattcgtcca acaacaactc
     2761 accctgactt gcgagcgtct ctgcgccgca ccaaatttgg acggacgtgc gccgcgtggt
     2821 gtgttttcgc agggactctg ccgtgtcgtg acggatttcg gaggacgcgc cgcgttgtac
     2881 tcgatttgga acgacgcgcc atcacctccg ccgggttgtc gattgatact tcattgtgtt
     2941 tcttaggcac ggcggcgcgt gatgctctat gatgagcaat catttcattt cgccatgaca
     3001 accaagcccc tggccggact gcgggatgac gtcggcctcc atgggcatac ggtccttttc
     3061 tcgcggcctt tggcacgtta aactatcgac cacatgtctg aggtgacgag ccgaagcagg
     3121 tgtgcttttt gcctgccaaa tcggcctcga ttgtgcttga ccgcatagcg gcattcgaac
     3181 cgtaacgatt tatcaccctc tggaccacag ggattgagga gaccggacca tggccaaaca
     3241 atgctgggtg attaccgatg ttgcccgcgg cattgccctc gacgaactca cgttgacatc
     3301 aggtgtaact tcggcggttc cgaaaggcgt tagcattaca aaacggacac tccacggcgg
     3361 cctacaagat ggcgtcgatg tggtggacgt tgacaatggt cgccttcgct tcactgtgat
     3421 cccaacgcgt gggatgggcg tttggcgggc atggtgcgat aacatccaac tcggctggca
     3481 ggcgccggtc catgggccag ttcatcctaa atgggtcaat ctttgggaac ccagcgggtt
     3541 gggctggctg gacggcttcg atgaactcct agtccggtgc ggtctggaaa gtaacggcgc
     3601 acccgaattt gacgacaagg gcatcctgcg ctatccgctt cacggcaaga tcgccaatcg
     3661 cccagcctgc cgcgtcgaag tctctatgga tagcgagacg ggcaatatca ccgtccgcgg
     3721 cgaggtctat gaaacccggt tattcttcac aaaattgaag ttggctaccg agattcgcac
     3781 aactgttgga gcatgctctt taacggtgac cgacactgtg acgaacgtcg ccgctgtgcc
     3841 cggcgagatg gaactccttt atcacatcaa ttttgggcca ccggtcttgg agccgggcag
     3901 caaagtcacc ttgcccatcc aaaagctggc gccacgtgac cccatcgccg cggccgagat
     3961 tgaccgctgg gatcagtatg ggccagagtc gccaggaaaa ccggaaacgg cctacttcgt
     4021 ccagccgctt ggggatggcc gagggcagac gctggccctg ctccacaacg aagccgggac
     4081 caatggcgtc cgcgtcgatt tcaatattaa ggagctacct tgcttcacac tttggaagaa
     4141 tgagcaaccc gtgtgcgatg gttacgtcac aggcctagaa cccggcgtca actttcccaa
     4201 tcgcaggtca tttgagaagg ccaaaggccg cgttgttaca ctccagccag gagaggcacg
     4261 gcgtttcacg gtggagatca cagcgcttgt cacgtccgat gcggtatcca ccgcccggga
     4321 ggaaatcctc cgtctgcaaa cacaggcccc gcccgaagtg cttcggcaac cgtgcctaga
     4381 ttggtcgccg tgaaaacaac cagggacatg ccaccacggt gtactttttt ctcggttcac
     4441 gcgccgtgta tggatcggcc ctgaggctgc gttagccagg acggatcagc gattttggta
     4501 gcgctgcatc atccgccgga tttcccgttg agattcctct tttttgatga tctcgcgttt
     4561 gtcgtgctcc ttccgaccac gacagagacc gaggagcacc ttggccaatc cccgtttgaa
     4621 atagattttt agtgggatga gcgtgagccc cttttgtgag gcctgcgacg cgaatcgctt
     4681 aatttcgcgt cgatgaagaa gtaacttgcg cggccgtttt ggctcatgat tgaaccgatt
     4741 ggcctgcgga aactccgcga tgtcgcaatt gtatagccag acctccccgt ccttcacgcg
     4801 ggcaaaggcc tcttcaatcg agacctttcc gttacgaagg ctcttcactt cgctcccacg
     4861 caaggcaata ccgcactcca gcgtttccaa gacatcatac tgatgcttgg cccgccgatt
     4921 atgggtcacc acacgctcat tgtcatgctc ggcatcaacc ggcttcttgc ttttcgcc
//
LOCUS       DAOSUT010000235         4825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7692_length_4825_cov_9.902935, whole genome shotgun sequence.
ACCESSION   DAOSUT010000235 DAOSUT010000000
VERSION     DAOSUT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_4825_cov_9.902935"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLD05_14830"
     CDS             <1..759
                     /locus_tag="PLD05_14830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOP78761.1"
                     /translation="VGTLTRPWLWWQFLTYGFTHSREPSHIFFNMLTLWFFGRDIEYL
                     LGRKEFLRLYLTMVVVAGVLWSAINYLQGLPSEIALVGASGAVVGIFVLFCLHYPRRT
                     ILLFFVLPVPAWVAGVLLLAPDVLSALRNQDSGVAYSVHLVGATIALLYYYQGWNFGQ
                     LWNRLGGLALWTRVKYLFQRRPRLRVRHPEVEEDDEDEQYSKEAAELEAEVERILDKI
                     NQSGIESLTRSERRTLERASRFFRERRHSSEKRG"
     gene            923..1882
                     /locus_tag="PLD05_14835"
     CDS             923..1882
                     /locus_tag="PLD05_14835"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78762.1"
                     /translation="MSQRIDRRRKGFTLVELLVVITIIAMLVSLLMPAVQMAREAGRR
                     TQCLNNQKQFATALANYESARRQFPGWRQVVSRDETNSVDVVGTWVIPLLPYLEQRQV
                     YDIWVDNGVPWANKPQIRLPIAICPSNPPEDMDVGPAVMMYVANAGLPDAGLGQNQIE
                     GPASAVFLDHTRSPRKSISLDYLGAHDGASNTLAISENIQATSWVPADSGAPRLPIEY
                     DLCMLWTPSPGGCGSSDAVAPINRCLGDSQSPSTPKLYLARPSSRHPGVVVVTYCDGH
                     GTTQRDNIDWLVYKQLMTPDNYIAGTFAGDNELRNSVYDSGNN"
     gene            complement(1931..3787)
                     /locus_tag="PLD05_14840"
     CDS             complement(1931..3787)
                     /locus_tag="PLD05_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78763.1"
                     /translation="MEVAINREMSLFVRTLIGLVLLLAGSFEPEVLQAGLGPENVLLV
                     VNEKSAASLTVANHYQALRQIPSENVFYLSWDPKEQITDIDRFREKILIPILQEIAKR
                     AVTAQIDCVLYSADFPWGIELAKDIERLIEEHPELLPPPKRTEASAEEEKESPGDRWP
                     RVLTRRGSINGLTYLYPGVLARRPDYYMSLRVNQYMRRAIPEQKDVPTLGFSSRYQFG
                     PHGEIVPNGERYLLSMVLAVVADDDTRGNTWEEILEYLSRSAAADGTFPPGTIYFVQN
                     GDIRSRTRDPAYPEVVEAIRAHGVRAAIVHGVMPMNKPDCQGVMMGTAEFDWKDSGST
                     ILPGAICDHLTSLGGVMTRSGGQTCLAEFLRHGAAAASGTVTEPFAIAEKFPMPTIHL
                     HYVRGCTAAEAFYQSVYGPYQLLIVGDPLCRPWANIPQVSLKGPADWETVRGVVTLQA
                     EARFPRGGLPAPLGGGQVDHFEWYVDGKRLGQSSPGEPFVLNTAARDDGYHELRVVAV
                     ETGLIATRGCVIQGIVTCNHGGQITARLLTPSVKLGEKILIDVDCPGALAVAIRHNSR
                     VVARALGDRARLTVDTGQLGTGPVRLHAVGIGKALSSNKISRPIDIVIGREG"
     gene            4263..>4825
                     /locus_tag="PLD05_14845"
     CDS             4263..>4825
                     /locus_tag="PLD05_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP78764.1"
                     /translation="MRLLTCIVALFLSGPVLAGEPSGCLTPEEQEQGFVCLFDGTSLE
                     NWQGDTKGYVIEDGVLVCKPGGNLYTKKEYADFIFRFEFKLTPNANNGVGIRTPMGCD
                     AAYCGMEIQILDDSGERYENLQPYQYHGSIYGVVPAKRGHLKPVGEWNSEEIYCKGRH
                     VKVTLNGTVIVDANLDEVEPMDHRDHPG"
BASE COUNT         1079 a         1268 c         1333 g         1145 t
ORIGIN      
        1 gtaggcaccc ttacccgacc gtggctgtgg tggcagttct taacgtacgg gtttacccat
       61 agtcgcgaac catcccacat tttcttcaac atgctaactc tttggttctt tggtcgcgat
      121 atcgagtatc ttttggggcg gaaagaattt ttgcgccttt atctgaccat ggtcgtggtg
      181 gctggggttc tctggagcgc catcaactat ctacagggct tgccttcgga gatcgctcta
      241 gtgggcgcat cgggagccgt ggtgggaatt ttcgtccttt tctgcctcca ttatcccagg
      301 cgaacaattc tgctgttttt cgttctaccg gtcccggcat gggtggccgg tgtgctattg
      361 cttgccccgg atgtcctatc tgccctgcga aatcaagaca gtggcgtggc ctactcggtc
      421 cacctcgtgg gagccaccat tgcattgctt tattactatc agggctggaa ctttgggcag
      481 ttgtggaacc ggttaggggg tcttgcgctt tggacccgcg tgaagtatct attccagcgg
      541 cgcccccggc ttcgcgttcg gcatcccgag gttgaagaag acgatgaaga cgagcagtac
      601 tctaaggagg ctgccgagct tgaggccgaa gtggaacgca ttctcgacaa aatcaatcaa
      661 tctggcatag aaagcctgac tcgctcagaa aggcgaaccc tggaacgcgc cagccgcttt
      721 tttagggaac gccggcactc gtcggaaaaa cgtggctaaa cggcatcgat ggaaacaagt
      781 ctcggattgg gtgaggaaaa catagggcga ctccgtgcct caaaaaactg aaatatacca
      841 cttgaaaatt taagttcggc gtgcgaaaat gaaagcgggc aagggttggg ttatgaaggt
      901 acgaatgtga tggaggtgcg ccatgtcgca acgtatcgac cgcagacgaa agggttttac
      961 gctcgtagag cttctcgtcg taattacaat tatagcaatg ctggtctctc ttctgatgcc
     1021 agcagttcag atggcgagag aagccgggcg tcgcacacag tgcttaaata atcaaaagca
     1081 gttcgcaacg gccttggcga attacgaatc ggcgcgacgt caatttccgg gctggcgaca
     1141 ggttgtctct cgggatgaaa cgaacagcgt agacgtggtt ggtacatggg taatcccttt
     1201 acttccttac cttgagcaac gccaggttta cgatatttgg gtagataacg gggtgccctg
     1261 ggcgaacaag ccccaaatta ggttacctat tgcgatttgt ccgagtaacc cgcccgaaga
     1321 tatggatgtt ggtccagcgg ttatgatgta tgttgcgaat gccggattac cagacgccgg
     1381 tttgggccag aaccaaattg aaggacccgc tagtgccgtt tttctggatc atactaggtc
     1441 gccacggaag tcgatttcat tagactattt gggcgcccac gatggtgcct cgaacacgct
     1501 ggcaatcagc gaaaacatcc aggcaacttc ctgggtgccc gcggattcgg gagcgccccg
     1561 tttgccaatt gagtatgatt tgtgcatgct atggacgccg tcacctgggg ggtgcggtag
     1621 ctccgatgcg gttgccccga ttaataggtg cttgggcgac tcgcagagtc cttccacccc
     1681 aaaattgtac ttggcgcgtc cttcgagccg acatcccggg gtggtggttg tcacgtattg
     1741 cgatggtcat gggacgacgc aacgagacaa tatcgactgg ctagtttata aacagcttat
     1801 gacaccggac aattacattg caggcacgtt tgctggggac aacgagttgc gaaacagtgt
     1861 gtatgattct ggtaacaact gagcagcatc ggctgaatta agcaggggaa cctgaaaagc
     1921 gaccgatttt ttatccctct cgcccgataa cgatatctat tgggcgggag attttgttgg
     1981 aactcaacgc tttgccgatc cccacggcat gaagtcgtac aggtcccgtc cccaattggc
     2041 cggtgtcaac tgtcaaccgg gcacgatccc cgagagctcg tgcaacaaca cgactgttgt
     2101 gtcggatcgc tactgctagc gctcccgggc aatcaacgtc gatcaagatt ttctcgccca
     2161 atttcaccga tggcgtgaga agccgagccg tgatctgccc accatgattg cacgtcacga
     2221 tcccctgaat gacacagcct cgcgtggcaa ttagcccggt ctcaacggcg acgacgcgaa
     2281 gctcgtgata gccatcgtcc cgcgctgccg tgtttaaaac aaatggttca cctggcgagg
     2341 attgccccaa gcgtttgcca tccacgtacc attcaaaatg atccacctgg ccgccgccaa
     2401 gcggggcagg caaaccgccg cggggaaatc tcgcctcggc ttgtagcgtc accacgcctc
     2461 ggacggtctc ccaatctgct gggcctttga gagaaacttg cgggatattc gcccatgggc
     2521 gacaaagcgg gtcgcccaca atcagcaatt ggtacggccc ataaaccgat tgataaaagg
     2581 cctctgccgc cgtgcatccc cgcacataat gcaaatgaat cgtaggcata ggaaattttt
     2641 ccgcgatggc aaaaggttcg gtcaccgtcc cacttgcggc tgctgctccg tgacgcagaa
     2701 actctgccag gcacgtttgc ccgcctgatc gggtcatcac tccgcctaaa ctcgtgagat
     2761 gatcacaaat agcaccgggc aaaatggtac ttcccgagtc cttccaatcg aactccgccg
     2821 tgcccatcat gacgccttgg caatctggtt tattcatcgg catgacacca tggacaatcg
     2881 ccgcccggac gccgtgagcc cgtatggcct caaccacctc gggataagcc ggatcacgag
     2941 tccgtgaacg gatgtcgcca ttttggacga aatagatcgt acccggggga aaagtcccat
     3001 ccgctgccgc acttcgtgac aggtattcta ggatttcctc ccatgtgttg cctcgtgtgt
     3061 catcgtcagc aacgacggcc aacaccatgg aaagcaaata ccgttctcca ttaggtacaa
     3121 tttcgccatg ggggccaaat tgatatcggc tactgaatcc caaggtcggg acatcctttt
     3181 gctccggaat ggcgcgtcgc atgtactgat tcacgcgaag agacatgtag tagtcggggc
     3241 ggcgggccag tactcctgga taaagatacg tcaacccatt gatggagcct cgccgcgtta
     3301 acacccgagg ccaacgatcg ccaggggact ctttttcctc ctcggcggat gcctcggtgc
     3361 gtttgggcgg cgggaggagc tccgggtgtt cttcaatgag gcgttcaata tcctttgcca
     3421 attcgatccc ccatgggaag tcggccgaat acagcacgca atcaatctgt gctgttacgg
     3481 ctcttttcgc aatttcctgc aaaatgggaa tgaggatttt ttcgcgaaag cggtcgatgt
     3541 ccgtgatctg ttccttcggg tcccacgaga gataaaagac gttttcggaa gggatttgac
     3601 gcaacgcctg atagtggttg gcgacagtca atgatgccgc gcttttttcg ttgacgacta
     3661 agagaacatt ttcgggccca agcccagcct gcaatacttc gggctcaaaa ctgcccgcta
     3721 agagtaagac caggccgatg agcgtgcgaa caaaaaggga catctcgcga ttgatggcaa
     3781 cctccattat gttcctttct ttggagaacg tatgtatccc tacctgaccg gggcgtttta
     3841 atgatcgagt ccctttgggc cgtaagccac aggcctcctg cgagattgcg ctgctaccag
     3901 aggccgattg cattagagcc tatcccataa tctcccctcg cccgttcggc gggagagggg
     3961 tcgggggtga gggtggctca aattgctgcc gcttttgtca atcgattccg atgttcgttt
     4021 accctcactc caaccctctc ccagagggag agggcgactt tttgagatag gctgttagta
     4081 aagggattac cgccatcgcc acctgtttca cgcggccttt ctgccaaagc gttttcactt
     4141 ggcaacagtt gcccccaagt gtcatctgcg atcgggttga ttatactcat agcaacgtgt
     4201 agctggtttc ccgtcaaggc gggtattctt tgctgaatgg ttcccgtgta aggagttgcc
     4261 tcatgagact gctgacctgt attgtcgcgc ttttcctcag tggaccagtg ctggcgggcg
     4321 aaccctcagg atgcttgacg cccgaggaac aagagcaagg atttgtttgc ctcttcgacg
     4381 gcacctcgct cgaaaattgg cagggggata ccaagggcta tgtgattgag gatggcgttt
     4441 tggtgtgcaa gcctggcgga aatctttata cgaaaaagga atacgccgat tttatctttc
     4501 gttttgagtt caaattgacc cccaatgcaa acaacggcgt gggcattcgc actccgatgg
     4561 gttgcgatgc ggcgtattgc gggatggaaa ttcagatact cgacgattcg ggtgagcgat
     4621 atgagaattt gcaaccctat cagtatcatg gatcgattta cggggttgtt ccagctaaga
     4681 gaggtcacct caagccggtc ggggagtgga acagcgagga aatctactgc aagggtcggc
     4741 atgttaaggt cacgcttaac ggaacggtca tcgtcgatgc caacctcgat gaggtggagc
     4801 cgatggacca tcgcgatcat cccgg
//
LOCUS       DAOSUT010000236         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7762_length_4778_cov_7.994495, whole genome shotgun sequence.
ACCESSION   DAOSUT010000236 DAOSUT010000000
VERSION     DAOSUT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7762_length_4778_cov_7.994495"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLD05_14850"
     CDS             complement(<1..451)
                     /locus_tag="PLD05_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOP78765.1"
                     /translation="MRWYILARKRIALLSVAVLCCLFCPTDGVFGQGEWEITPESEAA
                     LERGLAWLARNQGPEGNWDSNDLGLVSTGILAFLAAGHLPDRGPYGSTVRRAMDYVIR
                     NAQPSGLLNIAEARRGTYNHGLSTFVLGQAYGMTNDRRLGPLLDQALK"
     gene            complement(461..>1791)
                     /locus_tag="PLD05_14855"
     CDS             complement(461..>1791)
                     /locus_tag="PLD05_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOP78766.1"
                     /translation="EGRVLVTHDIVSIDGVLGNDISRNLRTNANSAALKYLFSVESPG
                     IHRPPSMPDREFNWLKEQVTFLPLDDCLVMHAQADLRLFDWAGTLSWSQQSGVSSDNT
                     SRAMVAMCPLLYQDRIICRRLTQRGSELVCLDLFDGHVIWAQRLGDYVVSDPWIQDEV
                     VQAVVGTGLEIENYQLTLVALDVRDGSVRQRSPLAVLNNVLDVPLDCHVVPQDRWFLI
                     QANGCLMLFDSLGRPIWLRSFPWLAPASSSWWHAQSWYTQRDPRPLIVGDHVFVSMRG
                     GWFVACVELKTGKLCWYQPMGHLRGLFAYADGKLYVETSGSMWILEATTGRKLGQYNW
                     PARAEKAVVAPTRLVMAFQSRSEERDRRHRRLVMQCIQLDDGTLLREIDLGLIDRELE
                     WIGPVMTHHGKVYILLARPNQPQRFEVYELVLAKDDPQLASADQISALHK"
     assembly_gap    1792..1891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1892..4002)
                     /locus_tag="PLD05_14860"
     CDS             complement(<1892..4002)
                     /locus_tag="PLD05_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78767.1"
                     /translation="MKLTICFLLAFWLTGSVAFPQTGEEPARLTRAWGIDFHPAWYNE
                     VWPARASQLAEAISAPLIPVSDNASSDATQREGNFVFRRVNGTFQIPSEFHAKKALKL
                     GLVKPGDVSLLFSNGDGGIICRFYPNYHQSWAAYQAVVDSLNDNRTWQPIALLATDQG
                     RYRQSGMGSVLVFWENSDLILACGGTRLLTVPFRDPPKEIRLAGTTLLRELRWLDGVP
                     PPPLDSERLFPDAFDHPTSPLFLASRMVVNGSIPASQPWERWPQSKEVTVHWDESGEI
                     GLSAEDVRERTYLGFPIVVNGVCDIVFRVREATPGTGVFLADKDRRPIGGLGFYRHTQ
                     SGRLLFGFHTAQDDNWERGADPNQLLAITDGSQWFRITVSAGVLRYWVSCDGKVWSPA
                     GPFNDHADRRPAFCGIFLTKGPTRKIVLRDLYVYEGLVPSDEKLARLTEEVPDSIATA
                     SDWERWCELVVRNCPDTSTLQEWRMACAFRSIAENPPAHLGQRILEQLWDDILSLDLP
                     MHVKLNCSAVFAREVSQVDWGIYERLTPRLRRLAWQLATDDPSRAFTVITEHLLRIPY
                     WAQWRLPVFFTDLYRHELFAALAKNDTEHLQQLGLRVLRASVPGNDAVSDELRYLAVS
                     TAARTQATDVMPNETPSQARQSLVIVPNREAYNFAQEIAAALADNDPRQVTQLILLAG
                     REEMRSLYPDPLDPGRYCSFPL"
     gene            complement(4108..>4778)
                     /locus_tag="PLD05_14865"
     CDS             complement(4108..>4778)
                     /locus_tag="PLD05_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78768.1"
                     /translation="FLFAKAPGRTALWFVPASCLVAAAFFLTNWIAHRSVIVPYGHRS
                     SDGGDNWYDFEYERQGRIVQSYWRNPVGVDRGERSPWVYAFHVLVGHHGIFSLTPLWL
                     LSAIGLLMGLCDPAFRQWRILWVIIIFISLVCIAFYLSRPETDRNYGGMTCCFRWVLW
                     LTPFWLIGLVPVVARLSVSKIGRWFALAVVILSCASAATPTWNPWTHPWLFQWLEAAG
                     WIQY"
BASE COUNT         1150 a         1340 c         1240 g          948 t
ORIGIN      
        1 ccttgagcgc ctggtcgagc agtggcccca accgtcggtc attggtcatc ccatacgcct
       61 ggccaagaac gaaagtcgaa aggccatggt tgtaggttcc ccgtcgggct tctgctatgt
      121 tcaacagacc agagggttgc gcattccgaa tgacgtaatc catcgcacga cggaccgtgg
      181 agccgtaggg gccacgatcg ggcaggtgcc ccgctgccaa aaaagcaaga atgcccgtac
      241 tgaccagccc cagatcgttt gaatcccaat ttccctcggg tccttgattt cgtgccaacc
      301 aagcgagtcc ccgttccagt gccgcttcac tttccggtgt gatttcccat tctccctgcc
      361 caaacactcc atcggttgga caaaagaggc aacacagcac ggccacactt aagagtgcga
      421 tacgttttcg cgcaagaatg taccacctca tcgtatgatc ctatttatgc aaagcactga
      481 tttggtccgc tgaggcaagt tgcggatcgt ctttcgccag aaccagttca taaacctcaa
      541 atcgctgagg ttgattgggg cgagccaaga gaatgtacac cttgccgtga tgcgtcatga
      601 ccggtccgat ccattccagc tcccgatcaa tcaggccaag gtctatctcc cgaagaagtg
      661 tgccatcatc aagttggata cactgcataa ccagacgcct gtggcgacgg tcacgctctt
      721 cagaacggga ctgaaaagcc atgacaagtc gtgttggggc cacaacggct ttttctgccc
      781 tggcgggcca gttgtactgt ccgagcttcc gtcccgtcgt ggcctctaga atccacatac
      841 tgccggacgt ttcgacgtat aatttgccat cggcataggc aaatagacca cgcaaatgcc
      901 ccatgggctg ataccaacat agctttccag tctttagctc cacgcatgcc acaaaccagc
      961 ccccccgcat gctgacgaag acgtgatcgc ccacgatcag gggccgagga tcccgttggg
     1021 tgtaccagga ctgggcatgc caccagcttg acgacgctgg agcaagccag ggaaaagatc
     1081 gcaaccaaat aggacggcca agagaatcaa aaagcatcaa acaaccgttt gcttggatga
     1141 gaaaccatcg gtcttgcgga acaacatggc aatccagggg tacgtcgaga acattattca
     1201 gaacagccaa aggtgaccgt tggcgaacac taccatcacg aacatcaaga gcaactaggg
     1261 tgagctggta attttcaatt tctaaacccg tgccaaccac cgcctgaacc acctcgtctt
     1321 gaatccaagg atccgagacg acgtagtccc cgagacgctg tgcccagatg acatgtccat
     1381 cgaatagatc caaacagacc agttccgaac cacgctgggt taaacgtcgg caaatgatgc
     1441 ggtcctgata caaaagtggg cacatggcga ccattgcccg actggtattg tcggaggaca
     1501 caccagactg ctgagaccaa ctcaacgttc cagcccaatc gaagaggcgg agatctgcct
     1561 gcgcgtgcat cacgagacag tcatccaggg gcaaaaaggt tacctgttct tttagccaat
     1621 taaactcccg atctggcatt gacgggggcc gatgaatgcc aggactttcc accgagaaga
     1681 gatatttcag ggctgccgag ttggcgtttg tccgcaagtt tcttgaaata tcgtttccca
     1741 agacgccgtc aatgctcacg atgtcatgcg tgacgagtac cctgccttcg tnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcgggaaa gaacaatacc ttccggggtc
     1921 taagggatcc gggtaaaggc ttcgcatctc ttcacgccca gccaaaagaa tcaactgtgt
     1981 cacctggcga ggatcgttat cggccagcgc cgctgcgatc tcttgggcga aattgtatgc
     2041 ctcacgattg ggcacgatca cgagggattg tcgggcttgg ctgggcgtct cattcggcat
     2101 gacgtcggtg gcttgggtcc gtgctgcggt cgaaaccgcc aggtaccgca attcatcgga
     2161 aactgcgtca tttcccggaa cactggccct taggacccgc agaccaagtt gctggagatg
     2221 ttcagtgtcg ttcttcgcca gggctgcgaa caactcgtga cgataaaggt ccgtaaaaaa
     2281 caccggcaat cgccattggg cccaataggg aatacgcaat agatgttctg taattaccgt
     2341 gaaagcgcga ctgggatcgt cggtggccag ttgccacgcc aaacgacgca aacgaggtgt
     2401 gagccgctcg tagatacccc aatccacctg ggaaacctct cgcgcaaaaa ccgccgagca
     2461 atttaatttc acgtgcattg gcaaatcaag gctgaggata tcgtcccaga gctgttccaa
     2521 aatccgctgc cctaagtgtg ccggtggatt ctcggcgatc gaccgaaacg cgcaagccat
     2581 gcgccattcc tgtagagttg acgtgtccgg acagttacga acgacgagct cacaccaacg
     2641 ctcccagtca gaggcagtcg caatgctgtc tgggacctcc tccgtcaatc tcgcgagctt
     2701 ctcatctgaa ggtacaagcc cttcatagac gtaaaggtct cgcagcacga tttttcgcgt
     2761 gggtcccttg gttaggaaaa ttccgcaaaa ggccgggcga cgatcggcgt gatcgttgaa
     2821 ggggcctgcc ggactccaca ccttgccatc gcaactaacc caatatcgca ggacacccgc
     2881 cgagacagtg atccgaaacc attgggagcc atcggtgatg gccagcagtt ggttggggtc
     2941 tgcaccacgt tcccaattgt catcttgtgc cgtatggaac ccaaagagta gccttcccga
     3001 ctgtgtgtgc cgataaaaac ccagtccgcc gataggccga cgatccttat ccgctaggaa
     3061 gacgcccgtt cctggtgtgg cttcgcgcac ccggaagaca atgtcgcaga cgccgttgac
     3121 gacgatcggg aagcctaaat acgtcctctc cctcacatcc tctgccgata ggccgatctc
     3181 gccgctttcg tcccaatgaa ccgtcacctc cttcgactgg ggccagcgtt cccacggctg
     3241 gctcgccggg atcgatccat tgaccaccat tcttgaggcg aggaatagtg gggacgtagg
     3301 atggtcgaac gcatccggaa agagcctttc actgtcgagc ggcggtggag gcactccatc
     3361 aagccaacgc agttcccgca gaagcgtcgt tcccgctaag cggatttcct tggggggatc
     3421 tcgaaatggt accgtcaaca gccgtgtccc accgcaggcc aaaataagat cgctgttttc
     3481 ccagaaaacc agaacgcttc ccatgccgct ttgccgatac cggccttgat ccgttgccag
     3541 gagggcgatt ggctgccacg tgcgattgtc attaagggaa tcaaccaccg cttgataagc
     3601 cgcccagctt tgatggtagt tcgggtaaaa ccggcatatg atgccaccgt cgccgtttga
     3661 gaaaagtagc gagacatcac ccggtttgac gagtcccagc ttgagggcct ttttagcatg
     3721 aaactcactg ggaatctgga acgtaccatt tactcgtcgg aagacgaagt ttccttcccg
     3781 ttgtgtcgcg tccgacgatg cgttgtcgga gaccgggatc agaggggccg aaatcgcctc
     3841 ggccagttgc gaggcgcggg ccggccagac ttcgttatac catgcgggat ggaaatcgat
     3901 tccccaagca cgggttaagc gagccggttc ttcccccgtt tggggaaagg cgacgcttcc
     3961 cgtcagccaa aacgcgagta aaaaacaaat ggtcaatttc attgtcagga ccatccgtcg
     4021 tgccggaacc gccgaaaatc gagctggttc gttcgactga ccttttgagc cagttggtgt
     4081 gctctcatat ctcaatcact gagacagtca gtattgaatc catcctgcgg cttccaacca
     4141 ttggaatagc cagggatggg tccaggggtt ccaggtggga gtcgccgcgg aggcacagga
     4201 gaggatgacc acggccaagg caaaccatcg tccaatttta gaaacactca ggcgggcaac
     4261 tacgggaacc aatccaatca accaaaaagg tgtcaaccaa aggacccaac gaaaacaaca
     4321 cgtcatgccg ccgtagttgc ggtcggtttc tggtcggctc aggtaaaacg caatacaaac
     4381 taaggaaata aatataatga tgacccacag gatacgccat tggcgaaacg cggggtcgca
     4441 aagtcccatg agaagtccga tcgcacttaa gagccacagg ggagtcaggg agaaaatccc
     4501 gtgatgtccc accaggacat gaaatgcgta gacccacggg gagcgctctc cgcgatccac
     4561 ccctaccgga ttccgccagt aactttggac gatccggcct tgacgctcat actcgaagtc
     4621 gtaccagttg tcgcccccat cactcgaacg atgcccataa gggacgatca ccgatcggtg
     4681 ggctatccaa ttcgtgagga aaaaggcggc agccacaaga caacttgccg gcacgaacca
     4741 gagtgctgtc cttcccgggg ccttggcaaa aaggaaaa
//
LOCUS       DAOSUT010000237         4688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7906_length_4688_cov_9.697172, whole genome shotgun sequence.
ACCESSION   DAOSUT010000237 DAOSUT010000000
VERSION     DAOSUT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7906_length_4688_cov_9.697172"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1559)
                     /gene="hslU"
                     /locus_tag="PLD05_14870"
     CDS             complement(222..1559)
                     /gene="hslU"
                     /locus_tag="PLD05_14870"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOP78769.1"
                     /translation="MSCLTPHQIVAELDKYIVGQTMAKRAVAVALRNRWRRQQLPPEI
                     RADIGPKNILMIGPTGVGKTEIARRLARLTGAPFVKVEATKFTEVGYVGRDVESMVRD
                     LVDNAISLLFQEYREKLKDEAEKRVENRILDILMKSPEDWDGLVASDEGARKNRVRTK
                     LRQLLREGVFDDRTIEIVTEKKELPFLVTGGGLEQIDIDFQSWAERLFAGKKSQRREV
                     TVKEAKKLFFDEEVEALINKDEIQAKAIELTENSGIIFIDEIDKIASVGVTHGPDVSR
                     EGVQRDLLPIVEGTTVKTRYGYVKTDHILFIAAGAFHRAKPSDLMPELQGRFPIRVEL
                     SDLTKDDFVKILTETETALVKQYQALLRPENVTLIFDSDAIELIAEYAYRVNQTTQNI
                     GARRLYTVMEKLLEDVSFDAPENISGEFRVTRDYVAAKLGDVIKDEDLSRFIL"
     gene            complement(1547..2089)
                     /gene="hslV"
                     /locus_tag="PLD05_14875"
     CDS             complement(1547..2089)
                     /gene="hslV"
                     /locus_tag="PLD05_14875"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOP78770.1"
                     /translation="MRIHGTTVLTVRRGDSVAIGADGQVSLGNAILKGDASKIRRLAD
                     GKVLTGFAGSTADALALYERFEAKLKEFPGNLPRAATELAKEWRTDRALRRLEAMLIA
                     VDAKHTLLLSGTGDVLQPSDGVLGIGSGGMYAVAAARALLRFTSLSAREIVEAALDIA
                     ADIDVYTNRNIIVEEISCRV"
     gene            complement(2462..3694)
                     /locus_tag="PLD05_14880"
     CDS             complement(2462..3694)
                     /locus_tag="PLD05_14880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOP78771.1"
                     /translation="MRFYSHILPNGLEVIAECSPSAFSTALGFFVRVGARDEPEDISG
                     VSHFLEHMAFKGSGSRTADDINREFDEIGAVYNAMTSYDSTIYYAAVLPEYMEKAFEL
                     LACLIRPAIRPDDFELEKKVILEEIRMYLDQPPYGIDETCRRRFFKTHPLGQSVLGTV
                     ESISALTPEQMRMYYQQRYTARNIIVGTTGCVDFDKLVALAERYCGDLNSEENERIQL
                     RPDPAFGTFRHHKPSATQQYVIQIAEAPPATSPERFAGRFLTAIVGSSTASRMYWEFL
                     DPGRADSVSLTYESYEDAGIFVTFLSCAPEMLEENLERLRKLYNQLETEGVSAEELSR
                     VKNKMAASLVLAAERPSSRLFAIGSERLTTGEYRSVEEELKIIDSITLDDVNRVAEKY
                     PLSCNATEIIGPMPQGSS"
     gene            complement(3681..>4688)
                     /locus_tag="PLD05_14885"
     CDS             complement(3681..>4688)
                     /locus_tag="PLD05_14885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOP78772.1"
                     /translation="EAVSRLHIVLTATMRASQLSEALPIFADVLLRPRLPREELEPAR
                     QGLIQEIRADRDLPEVKLRRELRRRCFPYPWNRPTQGEEETVAAIEWEDVRDFVDQHF
                     RPDGSIIAVAGRFDWDETKALVERLFRDWTGVAPSPLPWRWERERVKHVPYDSQQTHI
                     GVAYKSVPYDHPDYFLARASLGVLSGGTSARLFMEVREKRGLCYTVQATYHVIKNMAA
                     VFCYSGTTAERAQETLDVLLGEISRLKEGVQSEELERVKVQIKSSLVFEQESSASRAR
                     SLIGDWVLLGRVRPVTELKQSIDALTVDAVNRFLAENPPEDFTIVTLGPHALEVPREV
                     L"
BASE COUNT         1073 a         1390 c         1164 g         1061 t
ORIGIN      
        1 ttagagccta tcccaaagtc cccctctccc tctgggagag ggttagggtg agggcaagcg
       61 aacgtcggag tcggtcgcga aaaccggcag cgattcgagc caccctcacc cccgacccct
      121 ctgccgccga gcgggcgagg ggagattatg ggataggctt ttagttgtga ttactgcaat
      181 gagaaaatga gcctctccag tcccactcaa ggtcgatcat gctacaggat gaaacgactc
      241 aagtcttcgt ctttgatcac gtcccccagc tttgcagcca cgtagtcgcg cgtcacccga
      301 aattccccag aaatattttc aggcgcatcg aagctcacgt cttcgagtaa cttttccatg
      361 acggtataaa gtcggcgggc gccaatattc tgcgtggttt gattcacgcg ataagcatac
      421 tctgcaatca attcaatcgc gtccgaatca aatatcagcg taacgttttc cggccgcaga
      481 agtgcctgat attgcttcac caacgccgtt tccgtctctg tcaggatttt gacaaaatca
      541 tcttttgtca gatcagagag ttccacccgg ataggaaaac ggccttgtaa ctcaggcatc
      601 aagtcgctcg gcttcgcccg atgaaacgcc cccgcagcga taaacaggat gtggtcggtc
      661 ttcacataac catatcgggt cttcaccgtt gtcccttcca cgatgggcag tagatcgcgt
      721 tggaccccct cccgggaaac atcgggaccg tgcgtcactc cgaccgaggc aattttgtca
      781 atttcgtcaa tgaaaatgat gcccgaattc tccgtcaatt cgatggcctt ggcctggatt
      841 tcatccttgt taatcagggc ctccacttct tcatcaaaaa agagcttctt cgcttccttg
      901 actgtcacct cgcgacgttg cgactttttt ccggcaaaga gccgctccgc ccaactctgg
      961 aagtcgatat cgatttgttc caggccgcca ccagtcacga gaaaaggcaa ctcctttttc
     1021 tccgtgacga tctcgatggt gcggtcatca aagacccctt cccggagtaa ttgacgaagt
     1081 ttcgttcgaa cccggttttt tctcgccccc tcgtcagaag cgacaaggcc atcccaatct
     1141 tcgggtgatt tcatcaggat atcgaggatg cggttctcga cgcgtttttc tgcctcatcc
     1201 ttgagttttt cacgatactc ctgaaacaac aaggaaatgg cgttatcgac gaggtcacgc
     1261 accatactct cgacatcccg accgacatac cccacttccg tgaattttgt cgcctcaacc
     1321 ttgacaaatg gggcacctgt caaccgggcc aatctcctgg cgatttcagt tttccccaca
     1381 cccgtggggc caatcatgag gatattcttt gggccaatgt ctgcgcgaat ctccggcggc
     1441 agttgttgcc ttcgccaacg atttcgcagc gcaacggcaa ctgcccgctt ggccatcgtt
     1501 tgtcccacga tatatttgtc aagttcagcg acaatttgat gtggtgttaa acacgacacg
     1561 aaatttcctc cacaatgata ttccgattgg tgtaaacatc gatgtccgcc gcaatatcca
     1621 gcgcggcctc cacgatttcg cgagccgaca aactggtgaa cctcagaaga gcacgagcag
     1681 ccgcaacggc atacatcccc ccggatccga tgccgagtac cccatcggac ggttgaagaa
     1741 catcacctgt tccgctcaat aatagtgtat gcttggcatc gacggcgatc agcatggctt
     1801 ccagacggcg caaggcccta tcggtccgcc attccttggc aagctccgtg gccgcccgcg
     1861 gcaggttgcc tggaaactcc ttaagtttgg cctcgaatcg ctcatataag gccaaagcgt
     1921 cggcggtact tccggcaaac cctgttaaga cttttccgtc cgctaagcga cgaatcttcg
     1981 acgcgtcgcc cttcaggatg gcgtttccca ggctcacttg accgtcggcc ccaatggcca
     2041 cgctgtcgcc gcgccggacc gtgaggaccg tcgtcccgtg tattctcata gcaccaccta
     2101 agcgtgaaga aaatacgccg gcgcagccgg cggatactga aggcgtcctc gcaattgatc
     2161 ggaccaattc attaagttag cgccaaacac gcgggagcgt caagcggacg ccagaataac
     2221 gcgtgatcgt acttcccaac caaacctttg cattgactcc ccatttcgca gaaaggttag
     2281 cccccaaaag gattcgcgct tccggcgatt tggcgatcag acctgtcaca gtcgctgcgt
     2341 gacgtcattc tccacgacag tcagaaaacg ccaacggcga gcatacgaat gggacgaggt
     2401 gatttgccac gtcagccatt cctgatctga gctgcccgac ccgcgatgtg gaccatcgcc
     2461 atcacgaaga cccttgtggc atcgggccaa taatctcggt ggcgttgcag ctgagggggt
     2521 atttttcagc gacacgattg acatcgtcta aggtgatcga gtcaatgatc ttcagttctt
     2581 cctcaacaga acgatactct cccgtcgtga gtcgctccga tccaatggca aaaagccgag
     2641 aagatggtcg ctcagcagca aggacaagcg acgcggccat cttgttcttt acacggctga
     2701 gttcttcggc agaaacgcct tccgtctcaa gttggttata cagcttgcga agccgctcca
     2761 agttttcttc cagcatttcg ggggcacaac tcaaaaacgt cacaaaaata ccggcgtcct
     2821 cataactctc gtaggttaac gacacactat ccgcccgccc cgggtcgaga aattcccaat
     2881 acattcggct tgcggtacta ctccccacga tagccgtcag aaatcgcccg gcaaagcgtt
     2941 ccggggacgt ggccggcggg gcctcggcaa tttggatcac atattgttga gttgccgacg
     3001 gtttatggtg ccggaacgta ccaaacgccg ggtccggccg aagttggatt cgctcgtttt
     3061 cctcggaatt cagatcacca cagtaccgtt ccgcaagcgc caccagctta tcgaaatcga
     3121 cgcatcccgt ggtccccacg atgatatttc ttgcggtata tcgctgctgg taatacattc
     3181 gcatttgttc cggggtgaga gcagaaatcg actcgaccgt gccgaggacg ctctgtccca
     3241 ggggatgcgt cttgaagaat cgccgacgac atgtttcgtc gatgccgtac ggcggctgat
     3301 cgaggtacat gcggatttct tcgaggataa ccttcttctc aagttcgaag tcatcaggac
     3361 gaatcgccgg gcggatcaag cacgccagga gctcgaaggc cttttccatg tactctggca
     3421 gcactgccgc ataatagatg gtcgagtcgt acgaggtcat ggcattgtac accgcaccga
     3481 tttcgtcaaa ctcacgattg atatcgtcag cggtgcggct ccctgagcct ttgaaggcca
     3541 tatgctcaag gaagtggctt acgccggaga tatcttccgg ctcatcgcga gcccccaccc
     3601 gcacaaagaa tcccaacgcc gtcgaaaagg cagaaggcga acattcggcg ataacctcga
     3661 gcccattcgg gaggatatgg ctataaaacc tcacgaggta cctccaatgc gtgcggtccc
     3721 aaagtgacta tcgtaaaatc ctcaggcgga ttctcggcta ggaaacgatt gacagcatcc
     3781 acggtcaagg catcaattga ttgcttcagt tccgtcacgg gtcgtacccg acccagcagt
     3841 acccagtcgc caatcagaga tcgcgctcgt gaggcactgg attcctgctc gaataccaga
     3901 ctgcttttaa tttggacctt aactctctcg agttcctccg attgcacacc ctctttcaaa
     3961 cgggagatct cgccaagaag gacgtcaagc gtttcctgag cgcgctccgc cgtggtcccc
     4021 gagtaacaga agaccgccgc catgttcttg atgacatggt acgtggcttg aaccgtataa
     4081 cacaatccgc gcttttctcg cacctccata aagagacgtg cacttgttcc cccagataac
     4141 acaccaagtg aagccctcgc caaaaagtaa tccgggtgat cgtagggaac agacttatat
     4201 gccacaccaa tgtgcgtctg ctgcgaatcg taggggacat gtttgactcg ctcccgctcc
     4261 catcgccagg gcaggggtga cggagctacg ccagtccaat cgcggaataa cctttccaca
     4321 agggccttcg tttcgtccca atcgaaccga ccggcaacag ctatgatcga gccatcggga
     4381 cgaaaatgtt gatcgacaaa atctcgcaca tcctcccact cgatggcggc gaccgtctcc
     4441 tcctcacctt gtgtgggtcg attccaggga tagggaaaac atcgtcgtcg caattctcgc
     4501 cggagtttga cttctggcag gtccctgtcc gcccgaattt cctggatcag tccttgccga
     4561 gccggttcca attcctcccg agggagacgc ggtcgcaaaa gaacatcggc aaatatggga
     4621 agggcttccg acaactggct ggcccgcatg gtggccgtca atacgatgtg aaggcggctg
     4681 accgcttc
//
LOCUS       DAOSUT010000238         4677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_7933_length_4677_cov_9.052791, whole genome shotgun sequence.
ACCESSION   DAOSUT010000238 DAOSUT010000000
VERSION     DAOSUT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_4677_cov_9.052791"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1633
                     /locus_tag="PLD05_14890"
     CDS             377..1633
                     /locus_tag="PLD05_14890"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOP78773.1"
                     /translation="MMHKRVGCILLAITTALVVLFLNANQNGFALSEETTPLSQQYVI
                     LSEEELLDRIYGGWVGMLIGGLEGLPHEFKYVEKPSPTLPEFTFLANGARSDDDNDIE
                     WLHLWFMHQKGTLKIPYDELPAIWKANMNQGVWVANKRARELMDVGIVPPLTGSIEHN
                     PHAWYNLAGQFCVESYGLIAPGMPQTAADIGLHYARISVSQEPLQATQFWTTLISRAF
                     VLSLSTRELLEDALSAVDQRSDMAHAIRDAIAAHQKYPTDWKAARNEIHQRWYKARGW
                     NANSVIVNGAAVCLALLYGDGDFYQTLRYAMAIGYDADCNAATAGTVVGVRWGFRRIS
                     EHPQFRMPDRYQNRTRPQLPQEMRVSEQANILLQLARKVIVEQGGEVMQKDGQIYYRI
                     RQQEPKLLEPLPEVVKRPTPKDKAPS"
     gene            complement(1701..4274)
                     /locus_tag="PLD05_14895"
     CDS             complement(1701..4274)
                     /locus_tag="PLD05_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78774.1"
                     /translation="MPKYALILVCVILGVFADTSFSQVCNIKVVTDANPDYYDLDSLI
                     HSATGAWKTPEEKCWALFYWVHISRRQTSPMIFHGVEVTDPIRQFNDFGYTMCSTVAG
                     INCGLWHHMGFPVRFWDVTLHTVSECFYNERWHMYDNSMSAIYTLCDGKTVAGVEDLG
                     REGACEASEGRYETGHIVKYHCLTATSPNGFLTGADCARDLDQEARCFNPAGLKLRTY
                     YNNWEWGHRYVLNLYPGASYTRYYRSLGTTREYYVPNQGKDPESVNPRYRIRGNGIWE
                     FRPDLAPAGFSDAVHESINVIATPEKIVRPLEAGQVGHVIFKVQGANIITSQIIRAKG
                     FTKSSADRIEVSISTTVGRTWQKVCEIQSVGEFQIDCHLVDEVSGSYEVLVKFSLLAA
                     ENVEHVGISDIQIETRTMLNSKTQPLLRLGQNTVFVKLGEPTDTIVVWPDLQGENYRP
                     YVVAESNISTEPTHKGWHAVMFAQKPNEEAFTIYRIAAPRPLTRVIYGGRFYNRVPKG
                     SISLSHSWDGGTTWELDWQLTDTSQPWDVVHYVTLTNIPPGTRDVLLKYSMQSPQVGP
                     TACGIYSVRMEVQHQNNGPRFRPLDVTFAWDEIQADRTRVRRTHRQRIDSVPTRYTIN
                     VGGVDHPVMESLRIALVEDSPGATYGYSDESPGLVNRVLDVWQECGRNLLQGKPYTLS
                     AEPTGAWGADDPRRQKLTDGVVGSNYAGGTAMKYAAGFDEKSSPVDITVDMGELKTIT
                     ALGIHLTAGWPWWDALKGEVRDEIEAFTSRDGQTFESQGTFQLNLLRRDIPINYMLPD
                     DETLRAWNYILPLQSPVTAQFVRFRVTARRSLGITEVQAFDRLERRDFWMRILLPDEK
                     P"
BASE COUNT         1133 a         1287 c         1203 g         1054 t
ORIGIN      
        1 ggccttgtca tcaggaaaaa cagcttacca gggaaggtaa tcgcacgcta ttctgcggat
       61 tggctcgttt cgggaatcat taagattgct tggtccgaca aagacagcca attacggaag
      121 gtggccgcac atcttcatgc ttcgtgcgat ttaaccaacg cctcttgact ggcggtgtaa
      181 catcgcgcaa ccaagaattc cgttgtagcc aacaacacac gagagcttgc cactagtcgt
      241 ctctgctgcc tccgaaggat atggtgatga tcccgcctca tcaccgagtg ctagcacttt
      301 gacaataccc attcatttcc ctatcctaac acttgctagt cgcgcatgcc ctatgagaaa
      361 ttcgaaggag gtatccatga tgcacaagcg agtcggttgc atcttgctcg cgataactac
      421 tgccctggtg gtgttattcc ttaacgccaa ccaaaacggg ttcgccttgt ctgaagagac
      481 tacaccgctt tcgcaacagt atgtgatttt gtcggaagaa gagcttctcg accgcattta
      541 cggcggatgg gtaggtatgc ttatcggagg cctagaaggc cttcctcacg agttcaagta
      601 tgtagaaaaa cccagcccga cgcttcccga gttcactttt ctcgccaatg gggctcgctc
      661 ggatgatgac aacgatatcg aatggctcca cctctggttc atgcaccaaa aagggactct
      721 aaagattccc tatgacgaac tcccggcaat ttggaaggcc aatatgaacc agggagtttg
      781 ggtggctaac aaaagggcgc gggagcttat ggacgtagga atcgtcccac ccttgaccgg
      841 gtcgatcgag cataacccgc atgcatggta taacttggcg ggtcagttct gtgtggaatc
      901 atacggcttg atcgctcccg gaatgcccca gacggccgct gacattgggt tacactatgc
      961 ccggatttcc gtgtcacaag agccacttca ggccacccaa ttttggacga cactaataag
     1021 tcgcgcattc gtcttgtcgt tatccaccag ggaattgctg gaggacgcct tatcggccgt
     1081 cgatcagcgg agcgacatgg cccacgcgat tcgggatgcc attgccgccc atcaaaagta
     1141 ccctaccgat tggaaagcag cacgcaacga gatccatcag cggtggtata aagcgcgtgg
     1201 ttggaatgcc aattctgtca ttgtgaacgg cgcagccgta tgccttgcac ttctctatgg
     1261 agatggtgac ttctatcaaa ccctgcgtta cgccatggct atcgggtacg atgcagattg
     1321 caatgcagct accgcgggaa ccgtcgtggg ggtccgctgg ggattccgcc gtatttcaga
     1381 gcatcctcag ttccgcatgc ccgatcggta tcagaaccga acccggcctc agctcccgca
     1441 agaaatgcgc gtcagcgaac aggcaaacat cctcttgcaa cttgccagga aggtaatcgt
     1501 tgagcaaggt ggtgaagtga tgcaaaaaga cggccaaatt tactatcgca tccgtcagca
     1561 agagcccaag cttcttgagc cgcttccaga agttgttaaa aggccgaccc ccaaggataa
     1621 ggcgccgtca taggatacag gcactcaatg aactttgtga gcaaccggaa aagacaggcc
     1681 caagaccttc ggcgggtgaa ttagggcttt tcgtccggca aaagaattcg catccaaaag
     1741 tcacggcgtt ccaagcgatc aaaggcctgc acttccgtga tgcctaagct ccgtcgtgct
     1801 gtgacacgaa accgcacaaa ctgggcggtg acgggtgatt gaagcggtag aatataattc
     1861 caagcccgga gagtttcatc gtcgggcagc atgtagttga tcgggatatc ccgacgaagc
     1921 agattcaatt ggaaggtccc ctgcgattcg aaagtctgac catcgcgcga ggtaaaagcc
     1981 tcgatttcat cgcggacctc ccccttgagt gcatcccacc acggccagcc agccgtgagg
     2041 tgaatcccca acgcggtaat tgtttttaat tctcccatgt caaccgtaat gtcaacgggc
     2101 gaagacttct cgtcgaaacc cgccgcatac ttcatggccg tgccgcctgc atagtttgag
     2161 ccaacaacgc catcggttag tttctgccgc cggggatcat cggcacccca agcacccgtt
     2221 ggttccgcac ttaacgtgta cggcttgcct tgtagtaaat tgcgaccaca ttcttgccac
     2281 acgtcaagta cgcggtttac caaaccgggg ctctcatccg agtaaccgta tgttgcccca
     2341 ggagaatcct ccactaaggc aatacgaagg ctctccatca cgggatgatc cacaccaccg
     2401 acattgatcg tgtagcgcgt gggtacgctg tctatccgct gacgatgagt tcgccgcaca
     2461 cgcgttcgat cggcttgtat ttcgtcccaa gcgaatgtca catcgagtgg tcgaaaacgt
     2521 ggtccgttgt tttgatgctg aacttccatg cgaacgctat aaatcccaca cgccgtgggg
     2581 ccgacttggg ggctttgcat tgagtatttt agcaacacat cccgtgtgcc cggtggaatg
     2641 ttagtcaagg ttacatagtg aacgacgtcc caaggttgcg atgtgtccgt cagttgccaa
     2701 tccaactccc aggtggttcc tccgtcccaa gagtgcgaaa gactgatgct gcctttggga
     2761 acgcgattat aaaaccggcc gccataaatc acgcgggtta atgggcgagg agcggcaatg
     2821 cggtaaattg taaaggcttc ctcattcggc ttctgcgcaa acatcacagc atgccacccc
     2881 ttgtgggtcg gctcggtgga gatgttgctt tcggcgacga cgtacggacg ataattctca
     2941 ccctgcaaat caggccatac gacgatcgta tctgtcggct cccccaattt aacaaatacg
     3001 gtattctgcc ccaaccgtaa taggggctgg gtcttgctgt tgagcatggt tcgggtctcg
     3061 atctggatgt cggagatgcc aacatgctcg acgttttccg cggccaacaa ggaaaacttg
     3121 accaacacct cgtaactgcc agatacctca tccacgagat gacaatcaat ctgaaattca
     3181 ccgaccgact gaatttcaca cactttttgc caagttcgcc ctactgtggt actgatagaa
     3241 acttcgattc tgtccgctga gctctttgtg aagccctttg cccgaatgat ctgagaagtg
     3301 atgatgttgg ccccttggac cttgaagatc acgtgaccaa cctggccagc ctcaagcgga
     3361 cgaacaatct tttcaggcgt agcaatcacg ttaatagatt catgaaccgc gtccgaaaag
     3421 cctgcgggtg ccaggtctgg tcgaaattcc cagatgccgt ttccacgaat tcgatagcgc
     3481 gggttaacag attcggggtc ctttccttga ttaggaacat agtactcccg cgtggtcccg
     3541 aggctccgat agtaccgcgt atagctagca ccaggataca ggttgaggac ataccggtgt
     3601 ccccattccc agttattgta ataggtgcgg agcttaagcc ccgcgggatt aaaacagcgg
     3661 gcctcctgat ccaaatctcg ggcacagtct gcccctgtta aaaagccatt aggactggtt
     3721 gcggtgaggc aatggtactt gacaatatgg ccggtttcat aacgaccctc cgaagcctcg
     3781 caggcccctt ccctgcccag atcctcgacc cctgccacgg tctttccatc gcagagcgtg
     3841 tagatggccg acatggaatt gtcgtacatg tgccaacgct cgttgtagaa gcattcggaa
     3901 acagtgtgga gcgtgacatc ccaaaatcgc acgggaaatc ccatgtggtg ccacaatcca
     3961 caattgatgc ccgccaccgt actacacatg gtgtagccaa agtcattgaa ctgacgaatg
     4021 ggatcggtaa cttccacgcc gtgaaagatc atgggtgacg tctgacggcg cgaaatatgc
     4081 acccagtaaa acaatgccca gcatttctcc tccggcgttt tccaagcgcc ggtggcactg
     4141 tggatcagac tgtcgaggtc gtagtagtct ggattagcat cggttaccac cttgatgtta
     4201 cagacctgag agaacgaagt atcggcaaaa accccaagaa tcacacaaac gaggatcaag
     4261 gcatactttg gcatagcgac tctcctcaat acgactgaat acgacgtcgc agacgagagc
     4321 cattgtcagc gtcgcgtgca acaccgtcaa gtttttgttc gcagtttgaa ccggtttata
     4381 ttgtttccgt ctggcataaa cgggtgcgcc ccaaaagaca accgcaaccg acgggacgac
     4441 gcgttggtcc agatacctga cggaccgcca gattcgacaa agggtaacgg caagggttgc
     4501 ccctactggg aaggtcgtcc gcaaacggca gagataggcc ttgggcctgc ccgcggtaat
     4561 gagaagcccg ggccgtcatg gccgccataa cacggtggca cgcagatctg tcgcatctct
     4621 gactcgacca ctccttgggc atcagcacat tgggatactc ctcaactcct tgaatgg
//
LOCUS       DAOSUT010000239         4529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8213_length_4529_cov_9.356728, whole genome shotgun sequence.
ACCESSION   DAOSUT010000239 DAOSUT010000000
VERSION     DAOSUT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_4529_cov_9.356728"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLD05_14900"
     CDS             <1..519
                     /locus_tag="PLD05_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOP78775.1"
                     /translation="RGETGLAEFVKKFRVYDIGDQDGIADWMRREFPGMYYILSKAPP
                     GRDKRESTYRGMYLTGDISLTSRRWIEEHVRSCGPLGQLYPTKTSTAPNPHGCLKEGD
                     TPSWFFFLPLSGNDPNDPSKPGWGGQYRRGDDGWYHDLPITADFDPRTTVSQWRPVFQ
                     ADFAKRMRWCVE"
     gene            840..2060
                     /locus_tag="PLD05_14905"
     CDS             840..2060
                     /locus_tag="PLD05_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78776.1"
                     /translation="MSRTFRTSRRGFLKTTGAALALPYIITSSALGEGERPPASERIV
                     MGGIGIGNMGRADLGAFLRRPDVQYVACCDVRKSAREQAKATVDNHYQNNDCKAYNDF
                     RDLLARTDIDAVHVATPDHWHAIMVIEACRSGKDVYCQKPETLTLREGPLMIEAARRY
                     GRVVSGGSQRVLEDYREVVNKCWSGELGRIKWINVNVGPLSQPCYLPPEEVPDDIDWD
                     MWLGPAPWAPYNKARCDGNFSTSGGSWRSYIDYSGGGMTDWGAHHFGGATFAVDVREL
                     QPQEVIYHNEGGREFLEYRYPNGIVLYHNRPNTGNLQVEGTPGETREPKPVPGYKGTG
                     GIYGDFIECVKTREKPFRDIELAVNTMVVCHLGIIAYTLKRSLKWDAAKQQFVNDPEA
                     DRLRDRARREPWVL"
     gene            2128..2829
                     /locus_tag="PLD05_14910"
     CDS             2128..2829
                     /locus_tag="PLD05_14910"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78777.1"
                     /translation="MLEDAFEALKTFDWGADPKVLKPIDDAIVATYGDAAGRRELENK
                     LLELLKSDVSRAAKDFICRKLRVIGTAASVPVLAGMLTDPENAHMARYALERMECPEA
                     AQALRDALPKVNGALKIGVIGSLGARQDAASVPALAALMTDSNPGVARAAVLALGNIR
                     TAEAAQALTAASVAPEVRPAVADAMLACAEALLAAGNKVQAMALYKKLMSDDQPKHVR
                     LAATRGMLVATGKSE"
     gene            2969..4381
                     /locus_tag="PLD05_14915"
     CDS             2969..4381
                     /locus_tag="PLD05_14915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78778.1"
                     /translation="MLRTTAGLCVSLFFLFACGLGCSVLCLGEEASPPDTQLLQMVLE
                     LLREEDKDIRGLALEQIRNELKGEAVTRRLAEELPKLKSEAQVGLLGALVDRGDKAAR
                     PAIVSLLKETPDSAVRQAAMRALAFLGEPADAQLLLPFFLEGTDAERQAAQFALTRIP
                     GAEVGAAIIQAAERASAEQFRRLIALLLERRESAATARLLEWAGSGDPARRAAAVAAL
                     GDLAGPEQLPQMVRLVLATPPGRDREAVEKAVMLVCARIPDPEQRDASLLAAMKSLNE
                     TERVTLLPTLGRVGGKSALAMIEAAIQSQDAARHEAGIRAIANWPDGSVAERLIELAQ
                     HDPHPAHRITCLRALIRVAPLATDRSDEEKLSLLKTAMSLCTRDEERLLVLQRAQAIR
                     HVATLRYLMTFLDQPAYAEAACLSIVELAHHRDLREPNKAEFDKALDAVIRTSKDAVV
                     VERANRYKKGQTWVRPKGSQ"
BASE COUNT          920 a         1215 c         1455 g          939 t
ORIGIN      
        1 cgcggcgaaa ccggtttggc cgaatttgtt aagaagttcc gcgtgtacga cattggcgat
       61 caggatggga tcgcggattg gatgcggagg gagtttcctg ggatgtatta catcctttcg
      121 aaggcaccgc cggggcgtga taagagggaa tccacctacc gaggaatgta cctgacgggc
      181 gatatctcat tgacgagccg gcggtggatc gaggaacacg tccgatcttg tggcccattg
      241 ggtcaacttt atcccaccaa gacctcaacg gcccctaacc ctcatggctg tctgaaagaa
      301 ggggacacgc cgtcgtggtt cttctttttg cccttgagcg gcaatgaccc taatgaccct
      361 tcgaagccag gctggggggg acagtatcgt cgtggcgacg atggatggta ccatgatttg
      421 cccatcaccg ctgactttga cccgcggacc acggtgagcc aatggcggcc agtcttccag
      481 gccgatttcg ccaagcgaat gcgttggtgt gttgaataac ggaattgtgc tttgcgtttg
      541 tctcgaccga gcataggaat caccgggcga aatcggcaag tgcgcatggc agagattgaa
      601 atggccagcc tatgtgctgg taaaaggcta acctcagccg atgctggggg cgccgcttga
      661 ccaggtggaa ggcagtatct aagatgggcg ctctgagagg gctgtggggt ttgcccatgc
      721 gtggcaaggg cgaacatctt gcagactgcg atgatggagg agagcccctc atgcccgggt
      781 tagcctcgtt ctctgggtaa gtttgttgaa cgtgttcttt ttttaaatag gagaacgtca
      841 tgagtcgcac cttcagaaca agccgccggg gtttcttgaa gaccaccggg gccgcgttgg
      901 ctttaccgta catcatcacc agctcggcgc tgggcgaagg tgagaggccg ccagcaagtg
      961 agcgtatcgt gatgggcgga attggtattg ggaacatggg acgggctgac ctgggggctt
     1021 tcctaaggcg gccggatgtg cagtacgttg cttgctgtga cgttcgcaag agcgcccgcg
     1081 agcaggccaa ggctacggtc gataatcact atcaaaacaa tgattgcaaa gcttataacg
     1141 acttccgcga tctgcttgcc cggaccgata tagacgcggt tcacgtggca acccccgacc
     1201 attggcacgc catcatggtc atcgaagcct gccgcagcgg caaggatgtc tattgccaaa
     1261 agcccgagac gcttacactc cgcgaaggtc cgttgatgat cgaagccgcc cggcgatacg
     1321 ggcgagtcgt ctccggcggg agtcaacggg tcttggagga ctaccgggaa gtggtcaata
     1381 agtgctggag cggcgagctg gggcggatca agtggattaa cgtcaatgtg ggtccgctct
     1441 cgcagccttg ctatctgcct ccggaagagg tccccgacga catcgattgg gatatgtggc
     1501 ttggcccggc accctgggcc ccttacaaca aggcccgttg cgacggcaac tttagcacct
     1561 caggcggaag ctggcgatca tatatcgatt actctggtgg cggcatgacg gattggggcg
     1621 cgcatcactt cggtggggcc acttttgcgg tggatgtccg cgaattgcag ccgcaagagg
     1681 tcatttacca caacgaaggg ggacgcgagt tcctcgaata ccggtacccg aatgggattg
     1741 tgctctatca caaccgtcct aacacgggca atctccaagt cgaagggact cctggagaaa
     1801 cccgcgagcc gaagcctgtc ccgggctaca aggggaccgg cgggatttat ggcgatttta
     1861 tcgaatgcgt gaagacgcgg gaaaagccgt tccgggacat cgaactggcg gtcaatacga
     1921 tggtcgtctg ccacctgggg attattgctt acacgcttaa gcgatccctc aagtgggatg
     1981 ccgcaaaaca gcaattcgtc aatgatcctg aggctgaccg actccgcgat cgtgcccgtc
     2041 gtgagccttg ggttttgtaa ttggaacatc ggggatcagt gtgtgatatg agttaccagg
     2101 aaatccatga atttgcgagg atatgccatg ttagaagacg cctttgaagc cctgaagacg
     2161 tttgactggg gggctgaccc taaagtgttg aagccgattg atgatgccat cgtggccacg
     2221 tacggcgatg ctgcggggcg gcgagagttg gagaataagc tgcttgagct tcttaagagt
     2281 gatgtctccc gtgctgctaa ggactttatc tgtcggaaac tgagggtaat tgggacggcc
     2341 gcaagtgtgc cagtgttggc cgggatgttg accgatcccg agaacgcgca catggcccgt
     2401 tatgccctgg aacgaatgga gtgtcctgag gcggcccagg ccctgcggga tgccctgccc
     2461 aaggtaaatg gcgcgctaaa gatcggtgtg atcggatcgc tgggcgcgag gcaagatgcg
     2521 gcctcggtgc cagcgctggc ggccctgatg acggatagca acccaggcgt cgcccgggca
     2581 gcggtcctcg ctttgggcaa tatcaggact gcggaggcgg cccaggcgtt gacggcagcc
     2641 tcggtggccc cggaggtccg acctgctgtt gcagatgcca tgctggcctg tgcagaggca
     2701 ctcttggccg cgggcaataa ggttcaggcc atggccctat acaagaagct gatgagcgac
     2761 gaccagccga aacacgtccg cttggctgcc acacgaggca tgctcgtggc gaccggtaag
     2821 tccgagtagt cattaaacgc ttgtagtgtt tgctagagat gcggatgcgg cagttcccat
     2881 cccacgggat tgaggattac gtcgcggtgg ggtgaaaatc tgccgcatcc cttcctgtat
     2941 tctcgtggtt cattgggaga ccctagtaat gttgcgcact accgccggtt tatgcgtttc
     3001 cctgtttttc ctcttcgcct gtgggctggg ttgttcggta ttgtgcttgg gcgaggaagc
     3061 ctctccgccg gatacccaac tcctgcagat ggtcttggag cttctgcggg aagaggacaa
     3121 ggacattcgc ggattggccc tcgagcagat tcgcaacgaa ctgaagggcg aggccgttac
     3181 tcgacggctg gccgaggagc taccgaaact gaagagtgaa gcccaggtcg ggctgcttgg
     3241 cgccctggtg gatcgcggtg acaaagcggc acgaccggcg attgtgtcgc tcctcaagga
     3301 aaccccagat agtgccgtcc gtcaggccgc gatgcgggca ctggcctttc ttggcgaacc
     3361 tgcggatgct caactgctgt tgccgttttt cctggaggga accgatgccg aacggcaggc
     3421 tgctcagttt gctctgacgc ggattcccgg cgcggaggta ggagctgcca tcatccaggc
     3481 cgctgagagg gcgtcggctg agcagtttcg ccggttgatt gcgttgctcc tggaacgtcg
     3541 ggaaagcgcc gctactgctc gacttttgga atgggctggc tccggcgatc ctgcacggcg
     3601 ggccgcggcg gttgcggcac tgggcgactt ggccggtccc gagcaattgc cgcagatggt
     3661 ccgcctcgtg cttgctactc caccggggcg cgaccgcgag gcggtggaga aggctgtgat
     3721 gctggtctgt gcgcgcatcc ccgatcccga gcagcgtgac gcctcccttt tggcggccat
     3781 gaagtcgctc aatgagaccg agcgagtgac cctcttgccg acgttgggac gtgtcggcgg
     3841 aaagtctgcc ttagcaatga tcgaggccgc catccaatcc caggatgccg cgcgacacga
     3901 ggcaggcatt cgggctatcg ccaactggcc ggacggctcg gtggccgaac gtttgatcga
     3961 gttggcgcaa cacgaccctc atcccgctca tcggatcacg tgtctgcggg cactcatccg
     4021 tgttgcgccc ttggccaccg atcgtagcga cgaggaaaaa ctgtcgctgc tcaagaccgc
     4081 catgagtctt tgtacacgtg atgaggaacg gctcctcgtt ctccagcggg cgcaggcgat
     4141 acgtcacgtg gccacgctgc gatacttaat gacgtttctc gatcagccgg cgtatgcgga
     4201 ggcggcctgc ctatcgattg tggaactggc tcatcaccga gacctacgtg agcccaataa
     4261 agccgagttt gacaaggccc tggatgcggt catccgcacg agtaaagacg ccgtcgtggt
     4321 ggaacgagcc aatcggtaca aaaagggcca aacctgggtc cggccgaaag ggtcgcagta
     4381 aactgcctcg gacgccgttc cagcacaact ccggcagcga ggtagatccg gcagcggaat
     4441 aaaaggagaa gcccattagg aaaccggggc gggggtatgt tgttgcgcac gtgatgcgct
     4501 ttcactttga aactcctcag aaaatgaca
//
LOCUS       DAOSUT010000240         4522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8230_length_4522_cov_9.854712, whole genome shotgun sequence.
ACCESSION   DAOSUT010000240 DAOSUT010000000
VERSION     DAOSUT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_4522_cov_9.854712"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLD05_14920"
     CDS             complement(<1..1176)
                     /locus_tag="PLD05_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOP78779.1"
                     /translation="MSQSERAEFEPIPPFQGDVRYILLLTLVATLGGLLFGYDTAVIA
                     GAIDYMVEKFSLSATMKGWVTSNILLGCAAGAILAGPLSDLLGRRRLLLITAVLFAVS
                     AIGTAIPANLTQFVFFRILGGLAVGAAALVAPIYVAEIAPAHLRGRLVSLQQIAIISG
                     MVVVAVVNWFIALQGDHTWNVHWGWRWMFGSETLPAILFLFCLGLVPESPRWLIKQGR
                     TDEALKVLNRTSGSARAAWEIADIQRTIAEETGRIDELLRPGYRKILIIAIILAILQQ
                     VTGINAIIYYTPTIFRSSGSTDIMALAWTILTQAVNLTFTLVAIAVVDRLGRRPLLLL
                     TSVAMAVSLTLLGWAFHAKMSSTWVGIFVQCYLASFAIGMGPVVWVVLAEIFPTRTRG
                     "
     gene            1583..2737
                     /locus_tag="PLD05_14925"
     CDS             1583..2737
                     /locus_tag="PLD05_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOP78780.1"
                     /translation="MSPFLSIILGLASLGVGGEILVRGAAEIARRLRLSELVIGLTVV
                     AFATSSPELAVSISAAYRGYADVAFGNVVGSNIFNILFVLGLSALVAPLVVNRQLVLR
                     DVPIMIGVSLLTYWFAWNGRIEHHEGFILLALLVGYVALSLILARKSNRNEVSNAVQL
                     LKSDASSESQETEDPPQPRTGILGFFWDVAIFVGLLIVVGIGVAVLMLGAKLFVDGAV
                     LLARFWGVSELVIAVTVVAAGTSLPEAAASIVAGVRGRRDIAIGNVVGSNIFNILCVL
                     GFGTLVTQHGMNVAEEALASDVPIMVGVAVACLPIFITGGVVGRFDGFLLFAYYLAYT
                     ANLVLAVIHSPYLPAFRQVMLWAIIPLTALFLIVSLLHHVVRQRRLGRSG"
     gene            complement(2844..3680)
                     /locus_tag="PLD05_14930"
     CDS             complement(2844..3680)
                     /locus_tag="PLD05_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOP78781.1"
                     /translation="MSRKISFWQKWATKHASGVVVAVVRAWMRTLNYRVVYHDRSADP
                     ISPECRKPHLYLFWHEYILFPLYVREHSYLTMLLSAHRDADILSEVATRMGFYFVRGS
                     TRRGGDRAIRALFAAGRSRHLAITPDGPRGPRRTMAPGAIYLASRLGTPIVAIGFGYD
                     RPWRMNSWDRFAVPRPFSRARAIVSRPIVVPPDLSREGIEEYRTKIQNLLNELTAQAE
                     DWATSGATRADEERVVKAPASLPLHLLPQRIPQIDHVTDGLLLDDNPTLTQLKRATRL
                     AG"
     gene            complement(3812..>4522)
                     /gene="dapF"
                     /locus_tag="PLD05_14935"
     CDS             complement(3812..>4522)
                     /gene="dapF"
                     /locus_tag="PLD05_14935"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOP78782.1"
                     /translation="IGGDGLILILPSNVADARMRIFNADGSEAEMCGNGVRCVAKYVY
                     ERGIAKKNPLTIETGRGVLTLHLEVKDDRVQSVTVDMGEPILDPARIPVRLNGTVIEH
                     PINMDGVDLAITCVSMGNPHCVIFRPEIDDLLVTQIGPKLERHPLFPNRTNVEFVQVL
                     SPSEVRMRVWERGSGETLACGTGACAVCVAGVLAGRLDRKVLVHLLGGDLQIEWRSDG
                     HVFMTGPATEVFTGEWPD"
BASE COUNT          986 a         1221 c         1281 g         1034 t
ORIGIN      
        1 cccacgggtc cgcgtgggaa agatctcggc gaggaccacc cagacgacag ggcccattcc
       61 gatagcgaag gatgccagat agcactggac aaagattccc acccaggtgc ttgacatctt
      121 cgcatgaaag gcccagccga ggagcgtgag ggacacggcc atggccacgg aggttagcag
      181 taacaggggt cgccgtccca gtcgatctac cacggcaatg gcgacaaggg taaacgtcag
      241 attgaccgcc tgcgtgagta tcgtccaggc gagggccatg atgtcagtgc ttcctgacga
      301 gcgaaagatc gtcggcgtgt agtagatgat tgcattgatc cccgtcactt gttgaaggat
      361 ggcaaggata atcgcgatga tgagaatctt tcgataaccc ggcctaagga gttcgtcgat
      421 tcggccggtc tcttctgcga tggttcgctg gatatcggca atctcccagg ccgcccgggc
      481 agaaccgctt gtccggttta agaccttgag cgcctcgtcg gtccgtcctt gcttgataag
      541 ccaccgcgga ctttccggca caagtcccag gcaaaacaga aagaggatag ccggcagtgt
      601 ctccgagcca aacatccatc gccatcccca gtggacattc caagtgtgat ctccctgcaa
      661 agcaatgaac caattgacga cggcgacaac caccattccc gagataatgg cgatctgttg
      721 cagagagacc agtcggcctc gcagatgagc cggggcaatt tcggccacgt aaataggagc
      781 aacaagggct gctgctccaa cggcaaggcc gccaagaatc cggaaaaaga caaattgcgt
      841 gaggtttgcc gggatcgccg tgccaattgc ggagacggca aatagaacgg ccgtgatcag
      901 cagcagtcgg cgccgtccca aaagatcgct taggggaccg gcaagaattg ctcccgcggc
      961 gcaccccagt aagatgttcg aagtaaccca gcccttcatg gtggcgctca ggctaaattt
     1021 ctccaccata tagtcgatgg cgcccgcgat gactgccgtg tcataaccaa agagcaaacc
     1081 gcccaatgtt gccaccaggg tcaaaagcag gatgtaccgc acgtcccctt gaaaaggcgg
     1141 aattggttca aactcagcac gctcagactg agacatcgac gcacctttcg caacacggga
     1201 tagccaatcg ggccagattg ttttccaaat gcctgatatt tcacggtttt tcttggcgag
     1261 acgcaagagc ggtcgccggt cgtccatatt cagcacctaa gtcgatctgt ggtgatgcta
     1321 cccttcttgg ccacaacggg gtttcttacc tggagacaag aaacatcgtt ttcgccaggt
     1381 gagccgaagc cccccggtgg gtttcggcta tcgactcggt ttagcaatcg agctgtggaa
     1441 gctaaaataa aacactttga ttcgaaaagc ttacctgata cgaatttcac tgttgcgaca
     1501 agcatgtcac gaccattagc agtatttggc cacaggcagt cggcctaaac caattcagtg
     1561 gacagttcat aggcgaaaca tcatgtcccc atttctttca atcatccttg gtctagcgtc
     1621 cctgggagta ggtggtgaga ttctcgttcg cggggcggcc gaaatcgcta ggcggttgag
     1681 attatcggag ttggtcattg gtttaacggt ggtagcattt gctacgagct caccggaatt
     1741 ggccgtttcc atcagtgctg cgtatcgtgg ctatgcggac gtcgcatttg ggaatgtggt
     1801 gggaagtaat atcttcaata ttctgttcgt gttgggttta tccgccctcg tggccccgct
     1861 ggtcgttaat cgccagttgg tgctgcgtga tgtccccatc atgattggcg tttcgctgct
     1921 aacttattgg ttcgcatgga atggccggat cgaacaccac gagggtttta tccttcttgc
     1981 ccttctcgta ggatacgtcg ccctttctct aatcctagct cgcaagagca atcggaatga
     2041 agtgtcaaat gccgttcagt tgcttaaaag cgatgcgtcc agcgaaagcc aagagacgga
     2101 ggacccgcct cagccgcgaa cgggcatttt gggattcttc tgggatgtgg ccatctttgt
     2161 cgggttactc atcgttgtgg gcattggtgt ggctgtgcta atgctcggtg ccaagctgtt
     2221 tgtcgatggt gccgtactgc ttgctcgctt ttggggggtg agtgagcttg tcattgcggt
     2281 caccgtggtc gctgcgggta catcgctacc cgaagccgcc gcctcaatcg tagcgggggt
     2341 gcgaggccgc cgcgatatcg ccatcggaaa tgtggtggga agcaacattt tcaacattct
     2401 gtgcgtgcta ggatttggaa cgttggtgac gcaacatggc atgaatgtgg cggaggaggc
     2461 cctcgcttct gatgttccga ttatggtagg ggtcgcagtt gcttgcttac ccatcttcat
     2521 aacgggagga gttgtgggac gtttcgacgg tttcttgttg ttcgcttact acctggccta
     2581 tacggcgaac ctggttctcg cggtgatcca ttcgccttat ttgccggctt ttcggcaggt
     2641 catgctctgg gcaatcatcc cgctgacggc cctctttttg atcgtgagtc ttcttcacca
     2701 cgttgttcgt caaaggcggt tgggtcgttc gggatagcag aattgcggat ggaacttacc
     2761 gaaggcacgc ccatcaattt tccaagattc gcgtaccatg agaggggccg tgacatgtct
     2821 tcgtggggac cgtcggcgta aaatcacccg gcaagtcgcg ttgccctttt caattgggtg
     2881 agcgttggat tatcgtcgag aagcaacccg tcggtcacgt gatctatctg aggaatccgt
     2941 tggggaagca aatgcagcgg tagcgatgcc ggcgccttaa cgacgcgttc ttcgtctgcc
     3001 cgggtggccc cggaggtggc ccagtcctcg gcctgagcag tcaattcatt gaggaggttc
     3061 tgaatcttgg tgcgatattc ctcgattccc tcgcgcgaca gatcgggcgg tacaacgatt
     3121 gggcgactga caatggccct ggcacgtgaa aatggcctgg ggacggcaaa tcggtcccaa
     3181 ctgttcattc gccatggccg atcatatcca aacccgattg ccacaatcgg cgttcctagg
     3241 cgagatgcca ggtaaatagc tcccggggcc atggttcgtc gtggtcctcg cggtccatcc
     3301 ggtgtgatgg ccaaatgccg tgatcgccca gcggcaaata gtgcccgaat agcccggtca
     3361 cctccccgcc gtgtggagcc gcggacgaag taaaacccca tccgggtggc gacctcgctg
     3421 aggatgtctg cgtcccggtg tgcggagagt aacattgtga ggtaactatg ttcgcggacg
     3481 taaagaggaa aaagtatgta ctcgtgccag aacaggtaca agtgaggttt acggcattct
     3541 ggggaaatcg gatctgcact tcggtcgtga tacacaactc ggtagttgag ggttcgcatc
     3601 catgccctaa cgacggccac cacgacaccg ctcgcatgct ttgtggccca tttttgccag
     3661 aaagagatct tcctgctcat ctcaactcca tccttgagga ccgctggacc ctatgaaccg
     3721 atgttttgca aaactgggtt tcactcctgg ggtgctcaat atctggaacc agtagctact
     3781 ccacgagcaa cgagatccag tgccgttgct cctaatcggg ccactcgccg gtgaagacct
     3841 ccgtggctgg tcccgtcatg aagacgtgac cgtcggatcg ccattcgatc tggaggtctc
     3901 cgccgagcaa atggaccaag accttacgat ctaaccgacc tgcgaggacc cctgcgacac
     3961 aaaccgcgca cgccccggtg ccgcaggcca gcgtctctcc agagccgcgc tcccataccc
     4021 gcatccgaac ctcactcgga gaaaggactt gaacaaattc cacattggtg cgattgggaa
     4081 aaaggggatg cctttcgagt ttgggtccaa tctgagtcac cagaagatca tcgatctccg
     4141 ggcggaaaat gacacaatgg ggattaccca tcgaaacaca ggtgatggca aggtccacac
     4201 catccatgtt gatgggatgc tcgatgaccg tgccgttaag ccgcaccggg atgcgggctg
     4261 gatcgaggat aggttctccc atatcgacgg tcacactttg tactcggtca tctttaactt
     4321 ccaagtggag ggtgagcaca ccccgacccg tttcgatcgt cagcggattt tttttggcga
     4381 tcccacgttc atagacgtat ttggccacgc aacggacccc gttgccacac atttcggcct
     4441 cagagccatc ggcattaaag attcgcattc gggcgtcggc gacatttgag gggagaatga
     4501 gtatcagtcc atcgcctccg at
//
LOCUS       DAOSUT010000241         4504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8278_length_4504_cov_9.135986, whole genome shotgun sequence.
ACCESSION   DAOSUT010000241 DAOSUT010000000
VERSION     DAOSUT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_4504_cov_9.135986"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLD05_14940"
     CDS             complement(<1..326)
                     /locus_tag="PLD05_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78783.1"
                     /translation="MGRMKVYSGTVRVSLLLGLAVGIWAAIGLIGGPMGRLHQALADE
                     AVGPVVMEEGQAETQEAEAAKEEKPAEEAKPTEKEKPEAQKPTEKPKEPEKPAEEKKP
                     VAAEKPA"
     gene            complement(663..1631)
                     /locus_tag="PLD05_14945"
     CDS             complement(663..1631)
                     /locus_tag="PLD05_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78784.1"
                     /translation="MGPSVCASEVLYEKGDDMRMGFTLVELLVVIAIIGLLVAILLPA
                     VQAAREAARRAQCANHIKQITLGMHEYHDTVGAFPFGFSTLEQSWHAMLLPYVEQEAL
                     YKTLIFQETGPGNWDSGSANTRACQTVIPVFRCPSLPIPTHVDHDIPQRVPSSYNGIA
                     SSQATSDDLSSIPQGYPRVALEQIDLDGMLFGNSSIRFAEVVDGTSHTLLVGEMHTDP
                     DFKKDGQSMDYWHIGLPHTGNWVPGGTGGTEYSEAIASTYPPINGWKDSELPGVVMEL
                     SCGSYHPGGAMFGFVDGSVRFIQETIDISVYRALGSRNGQESITGF"
     gene            complement(2018..2728)
                     /locus_tag="PLD05_14950"
     CDS             complement(2018..2728)
                     /locus_tag="PLD05_14950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOP78785.1"
                     /translation="MMRVPFWSALEERQKTAAVTLFLSTVVMITWRYFGSAEFYREHL
                     ADRLALGLDPRAAAAWYRFVTGFALLGLMPALIGGHLLGRGLASYGVRLGILVRTVRS
                     AALLAPIFIFLGWISSHDPSMARCFPTNPAAGSSLAMFALHAAIYLLHYAGWEFHFRG
                     FLQMGLRDSLGDGNALLVQVMASTLVHIGSPAIETYGAIAGGILWGILAYRTQSLLSG
                     MVQHACLGIALDAFLCFR"
     gene            complement(2737..3777)
                     /locus_tag="PLD05_14955"
     CDS             complement(2737..3777)
                     /locus_tag="PLD05_14955"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP78786.1"
                     /translation="MAEEGKVLVTGATGFIGTRLVQALVERGRSVRALCRRDNVTSPP
                     GLGLRDGAPLRHPKVELFRGDVTDAESVNRAMEGCTEVFHVAGYAKNWAPDPTTFYRV
                     NRDGVKNVLAAAQRCGVRRVVWTSTIQTFPPTPPGQIGDETWVNRGPFLTDYEASKVA
                     AEKEALAMARQGLDVVIVNPTRVFGPGHITEGNSLSRLIDMYDRGRLPFLLNAGRNIG
                     NYVFVEDVVEGHLLAMERGRSGEKYILGGENVSLREFFRLVDQVSDKRHFQIPIFRPG
                     ALTFAWVQKKLAEWFGIYPAITPGWVRTFLMDRAYTCRKAQEELGYQPRPLIEGLRIT
                     YAWLQRVREEQD"
     gene            4433..4504
                     /locus_tag="PLD05_14960"
     tRNA            4433..4504
                     /locus_tag="PLD05_14960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4466..4468,aa:Arg,seq:tct)
BASE COUNT         1096 a         1359 c         1153 g          896 t
ORIGIN      
        1 gccggttttt ccgccgcgac gggctttttc tcctcggcag gtttttctgg ctccttaggc
       61 ttctcggtgg gcttctgcgc ttccggtttt tccttttctg tgggtttagc ctcttcggca
      121 ggcttctcct ctttagccgc ttctgcctcc tgggtttcag cttgcccctc ttccatcacg
      181 acgggcccaa ccgcctcgtc agccaacgcc tggtgaaggc gccccattgg tcccccgatc
      241 agtcctatcg cagcccatat tccgaccgcc aaccctagca agagggaaac cctgactgta
      301 ccactgtaaa ccttcatgcg gcccataaaa gaagctcctc tcatgcgcaa aaactcaatc
      361 cacgatacct ttgtcagaag gcttcgcaat tcctgactgc taacggaatg acacaagacg
      421 taaccaatta accataggtc atgccccggt aacagcgcgt cggggggatc gttttccccg
      481 cgggcggcga aactcccctc aaacaacata cggcaattgg ctcatcatag tcgttttatt
      541 tacgcttgtc agcatatctt gtctagactt ccaaatcatt acaaccagaa tcctccaagc
      601 aactccacct tagcccgcaa actggttccc ggatagcgca caaaaagatg gggaaatgaa
      661 cgttaaaagc ccgtgatgct ctcctgcccg ttgcgagagc ccaacgcccg ataaacagag
      721 atgtcgatcg tttcctggat gaatcgcacg gagccatcga caaacccgaa catggctcca
      781 ccaggatggt agctgccaca tgaaagttcc atgacgacgc cgggtagttc ggaatccttc
      841 cagccattga tgggcggata ggtcgaagca atggcctcgg agtactcggt tccgcctgtc
      901 ccaccaggca cccagtttcc tgtatgaggt aggccgatgt gccagtagtc catggactgg
      961 ccatcctttt tgaaatcggg atctgtgtgc atctcgccga caagcaaggt gtggcttgtg
     1021 ccgtcaacga cctccgcaaa ccggatggag ctattgccga agagcatccc atccaggtca
     1081 atctgctcta acgccacccg cggataaccc tggggaatac tactcaaatc gtccgaggtt
     1141 gcctgcgacg aagcaatccc gttgtagctg cttggaacgc gctgggggat gtcgtgatcc
     1201 acatgggttg ggatgggcaa actgggacac cggaacaccg ggatcacggt ttgacatgcc
     1261 cgcgtattgg cggagccaga gtcccaattc cctggtcccg tctcttgaaa gataagggtc
     1321 ttataaaggg cctcctgctc gacatacgga agaagcatgg catgccagga ttgttccaag
     1381 gtactaaaac caaagggaaa cgcccccacg gtgtcatggt attcatgcat ccccagggtg
     1441 atttgcttta tatgattggc acactgagcg cgacgagcag cttcacgtgc cgcttgcacc
     1501 gccggaagaa gtatcgcaac tagaagccca ataatggcga tcaccaccag caattcgact
     1561 aacgtgaagc ccatcctcat gtcgtcccct ttttcgtaaa gcacctcact tgcacatacg
     1621 gatggcccca tcttaaaaaa cccacctctt gcagacaacc agtcggcggc aaacgtcaac
     1681 aaactctctt tggagcccaa agcgaagaca acctagtggg atagagcgta tcaacgatct
     1741 ggtaacgacg gggtttcgtg cttactcttt gacatcgcgc agattctgcc catccataaa
     1801 gacctcagga gaagacggtt tctgcgggcc gaacttgagg accgtcccag gcttcgagtc
     1861 tatcccaaaa agttcccctc tccctccggg agagggttgg ggtgagggca aacgatcgtc
     1921 ggaatcgatc gacaaaaccg acagcgattc gaggcaccct caccctcgag cccctctccc
     1981 gccgaacggg agagggaaga ttagggggga tagaccctta gcggaaacac aagaatgcgt
     2041 ccaacgcgat acccaggcag gcgtgctgga ccatccccga cagcaacgac tgcgtgcgat
     2101 acgccaaaat gccccataaa atgccaccag cgatggcacc atatgtctca atggccggtg
     2161 aaccaatatg gaccaatgtg gaggccatca cttggactaa tagggcgttc ccatcaccca
     2221 aagaatcgcg cagccccatc tggagaaacc cgcggaagtg gaactcccaa cctgcgtagt
     2281 gaagtaaata gatcgccgca tggagtgcga acatggccag agaacttccc gctgcggggt
     2341 tggttggaaa acagcgggcc atcgatggat catgagacga gatccagccg agaaaaatga
     2401 agatgggagc aagcagcgcg gcacttcgca ctgtgcgaac aagaatcccc agtcgcacgc
     2461 catagctggc caaacctcgc cccaagaggt gaccgccgat caacgcgggc ataagaccca
     2521 acaacgcaaa acccgtgaca aaccgatacc atgcagccgc cgcccgcgga tcaagtccca
     2581 acgccaagcg atcggccaaa tgttcccgat agaactccgc ggagccaaaa tacctccacg
     2641 tgatcatcac cactgtggag aggaacaacg tgacggcagc ggtcttctgc cgctcctcaa
     2701 gggcagacca aaacggcact cgcatcatcc agtctctcaa tcttgttcct cccgcactcg
     2761 ttggagccag gcatacgtga tgcgcaatcc ttcgatcaag ggtcgcggct gataaccaag
     2821 ctcttcttgg gccttacggc atgtgtatgc gcgatccatc aaaaacgtcc ggacccagcc
     2881 aggggtaatg gccggataaa tgccaaacca ttccgcaagc ttcttttgaa cccacgcgaa
     2941 cgtcagggca ccaggccgaa atatcggtat ctgaaaatgc cgcttgtcgc tgacctgatc
     3001 caccaatcgg aagaattctc gcaggctcac gttctcgcca cccaggatat acttttcacc
     3061 actccgaccc cgctccatgg ccaacagatg cccctcgacc acgtcttcca caaacacata
     3121 atttccaatg ttccgaccgg cgttgagaag aaacggtaaa cgcccacgat cgtacatatc
     3181 gatcagtcgt gagagcgaat tgccctcagt gatatggccg ggaccaaaga cccgtgtcgg
     3241 attgacaatg accacatcga ggccttgtcg ggccatagcg agggcctctt tctcagcggc
     3301 aaccttactg gcctcatagt cggtcaggaa gggaccgcgg tttacccagg tctcatcacc
     3361 aatttgaccc ggaggcgtcg gggggaaggt ctggatcgtc gaggtccaca caacccgccg
     3421 caccccgcaa cgttgggccg ccgctaatac atttttgact ccgtctcgat tcactcgata
     3481 gaacgtggtc gggtccggtg cccagttttt ggcgtagccc gctacatgga agacctcggt
     3541 acagccctcc atcgcccggt tgactgattc tgcatcggtg acatcacccc ggaataactc
     3601 taccttcgga tgccgtaacg gtgccccatc acgcaacccc agacctggcg gagaggtgac
     3661 gttgtcacgc cgacacagag cacgcaccga gcgtccccgc tcgaccaaag cctgaaccaa
     3721 tcgggtgcca ataaatccgg tcgcacccgt caccaagact ttgccttcct cggccatgct
     3781 ctagtatcgc tcctaccagc tttgccgcgt gtccgctctc ccgtcacgaa gctacaagcc
     3841 gcacccttcg gcccaacctg ccacccgccg ccgcgggcga ttatatgcca ccccatccca
     3901 accgtcaaac agcagttttg ggaagtttag gtaacgatcg cacgctacag tgaaactgag
     3961 ccgaatacgt gctcggtggg ttgaacggtt gggcaacttg gcgaagctag ggaccccccc
     4021 gaagaagagg ctgcctagcc gtgacggtaa cccttgccgg gtgtcatgct ggccgataat
     4081 gtgaaatgag gtgaaccggg tagaaccgcc gccaaatggg gggacgtggg cgaaaactca
     4141 gtaccatcca cacatcagta acaaacggcc cagtgcagaa cggcgatagt tccgctgtgg
     4201 tccctgaagc accgattcgg gccgatgttc gccgtcgccc gtaattgggg gaaacccgac
     4261 gcttaagctt ccagacaatc tggcaactca ggctttaggg gttggctgcg aaaacaagtg
     4321 ggattaacaa tttgaaagca aaaagaatct ggcaatactg ggaacattgg caagctggat
     4381 aaccaaaaag gcattgccta ggtgagtgta ggaaagatat cgactatcac tcgcgcccgt
     4441 agctcaaggg atagagcagc ggatttctaa tccgctggtt gggggttcga atccctccgg
     4501 gcgt
//
LOCUS       DAOSUT010000242         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8364_length_4465_cov_9.536961, whole genome shotgun sequence.
ACCESSION   DAOSUT010000242 DAOSUT010000000
VERSION     DAOSUT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8364_length_4465_cov_9.536961"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLD05_14965"
     CDS             <1..901
                     /locus_tag="PLD05_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78787.1"
                     /translation="LSGRQIFMKIVIASGKGGTGKTTVATNLAFLLAREGLRVAYVDC
                     DVEEPNGHLFLQPVIEREWPIEKPVPVVESQRCTQCGRCSKACRFGAIISLPQRTVVF
                     PELCHACGGCQLACPAGAISEKGRRVGTVRAGYAGTIQFLGGELDIGQPMSPPIIRAL
                     KETPLEADLVFLDAPPGTSCPVVETLRGADMALLVTEPTPFGLYDLQLAIRLVRALSI
                     PAAVIVNRVVKDGEPFLQWRQRLKQLPVPVWAEIPESRAIAEAYSSGTIIAQNVPEFA
                     RELQPVMHKLKASIDEGTCRHQW"
     gene            873..1847
                     /locus_tag="PLD05_14970"
     CDS             873..1847
                     /locus_tag="PLD05_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOP78788.1"
                     /translation="MKELVVISGKGGTGKTSLTAALAVLSGPCVVTDCDVDAPDLHLV
                     LQPQTIQQQPFVGGRKACIITSTCRKCGKCVELCRFGAIRHKKGDAECEEGGFLIDAL
                     ACEGCGVCVDHCPERAIELRPYVNGQWYLSRFHGGYLFHATLDVGSENSGKLVTQLRR
                     AAHEFATKRGVPLILTDGPPGIGCPVIASLTGASLALIVTEPTVAGLHDFERIARLCR
                     QLGVPAVTVINKADINPETSDRIANSSIRYGTDVIGMIPFDSDIVRAQMAARSVVEVS
                     DGPAARAMRAIHSTVMSRLEKALDVHSGEFITIDVTQIDNVKSKGVTT"
     gene            1844..2182
                     /locus_tag="PLD05_14975"
     CDS             1844..2182
                     /locus_tag="PLD05_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOP78789.1"
                     /translation="MRIAIPLSGKTLSSHFGHCEQFAFVEVDDQAWQVKGVTFVQPPP
                     HQPGLLPRWLHEQGVTTVIAGGMGPRAQQLLSELGIDVILGATGLHPEKLAADYLAGQ
                     LAIGENRCSH"
     gene            complement(2215..3906)
                     /locus_tag="PLD05_14980"
     CDS             complement(2215..3906)
                     /locus_tag="PLD05_14980"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOP78790.1"
                     /translation="MVFLRCGSAVCAWGVKLFGVLALGLGAGPMQSILGGERPNIVII
                     LVDDMGWSDIGCYGSEIPTPHLDSLAKNGLRFTQFYNTGRCSPTRASLLTGHYPHQAG
                     MGHLDGTFVPNHPGYQARIADSSVTMAEVLKEAGYFTAMTGKWHLGQNRGTPPWSRGF
                     MRSLNLAAGGVHFPGQTWRKEAKLYLNGEEKAPDDPLFGDNWYGADLWTEWGLKFIDE
                     ALDAKKPFFLYLAHCAPHFPLMAPSADIAKFRGQYLVGWDKLREDRYRRQIEMGLIDP
                     RWPLSPLPENTPPWESVSEAERDRFDQIMAIYAAMIYAMDRSVGTLVAGLKQRDVLDN
                     TVIFFLSDNGGNAESGPNGRCEGDNPGDRHSVVFLGQNWATLNNTPFRKWKHYVHEGG
                     SATPLIVHWPAGLDAGQRGQLYHQPAHVIDLMPTVIQLAGATYPTQYNGHTIEPMEGV
                     SLVPAFSGKPIVRSQPLFFNHEQNRAVRDGKWKLVALAGQPWELYDMEADRTELHDLS
                     AQAPERVAAMAAQYEAWARRTHVIVEGIPDPFDKPARKVQAQKRQQPSQVAPRTP"
     gene            complement(4118..>4465)
                     /locus_tag="PLD05_14985"
     CDS             complement(4118..>4465)
                     /locus_tag="PLD05_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78791.1"
                     /translation="VAYTANVAMFLTTRHVIEGNVSQASKAFALLAAFGSNIVHECVP
                     ASALKRHGRGIVRTTRAAAWALMDYQQRGLVSREALLRLAESLRGPASSDIQAIVSRI
                     CQVFDTMQIQYER"
BASE COUNT          985 a         1198 c         1275 g         1007 t
ORIGIN      
        1 cttatcagga cgtcaaatat tcatgaaaat cgttatcgct agtggcaaag gtggtaccgg
       61 aaagacaacc gttgcgacaa atctagcgtt tcttctcgcg cgggaaggct tacgagtcgc
      121 ctatgttgat tgcgatgtgg aagaacctaa cgggcatttg tttcttcagc cggtcatcga
      181 gagggaatgg ccgattgaaa agcctgtgcc ggtggttgag tcccaacgct gcacccagtg
      241 tggccgctgt agtaaggcct gtcgctttgg agcgatcatc agcctaccgc agaggaccgt
      301 tgtctttccc gaattgtgtc atgcctgcgg cgggtgccaa ttggcttgcc ctgccggggc
      361 gattagcgaa aagggccggc gggtcggcac cgttcgggcg gggtatgccg ggaccattca
      421 gtttctcggt ggtgaactcg acattggcca gccaatgagt ccccccatta ttcgcgcctt
      481 gaaggaaaca cccttggagg cggatctggt ttttcttgat gctccaccag ggacgagttg
      541 tccggtagtg gagacgttac gcggagctga tatggctctt ttggtgacgg agccgacacc
      601 ctttggcctt tatgaccttc aactcgcgat tcgtttggtg agggcattga gtatcccagc
      661 ggccgtcata gtcaatcgcg ttgtcaaaga tggtgagcca tttcttcaat ggcgtcaacg
      721 attgaaacaa ttgccagtac ccgtgtgggc tgagatcccg gagagtcggg cgatagccga
      781 ggcctattcg agcggcacaa tcattgccca aaatgttcct gaatttgcaa gagagttaca
      841 acctgttatg cacaaattga aagcatcaat cgatgaagga acttgtcgtc atcagtggta
      901 aaggcggaac gggcaagact tcgctcacgg cggcgctggc ggtcttgagt ggcccatgtg
      961 tcgtgaccga ctgtgacgtg gatgcacctg atcttcatct ggtcttgcag ccacagacga
     1021 tccaacagca gccattcgtg ggtggacgta aggcttgtat catcacttca acttgccgta
     1081 aatgcggtaa atgtgtggag ctttgccgat tcggcgccat ccgtcacaaa aaaggtgatg
     1141 cggaatgcga agagggcggt ttccttattg atgccctggc ttgtgaaggc tgtggcgtgt
     1201 gtgtcgatca ttgtccggag cgggccatcg agctacgacc ttatgtcaat ggtcagtggt
     1261 acctaagtcg gtttcacggg ggatacctct tccacgcgac gcttgatgtg gggagtgaaa
     1321 actctggtaa actcgtgacg caacttcgca gagccgctca cgagtttgca acgaagagag
     1381 gggtgccgtt gatcctgacc gatggcccgc cggggattgg ttgcccggtc atcgcgtctt
     1441 taacgggcgc gagtttggcc ctgattgtga ccgagccaac cgtagccggg ctgcacgatt
     1501 tcgaacgcat tgcgcggctt tgccggcaac ttggtgtgcc cgccgtgact gtcatcaaca
     1561 aagcggatat taatcctgaa acgtctgatc gcatcgccaa cagcagcata agatacggaa
     1621 cagatgtaat cggtatgatt ccttttgatt ccgatattgt gcgtgcccaa atggcggcgc
     1681 ggagtgttgt ggaagtctcc gacggtcccg ccgctagggc gatgagggct attcattcca
     1741 ccgtcatgag tcgccttgag aaggctcttg atgtccacag cggtgaattc ataaccatcg
     1801 atgtaacgca gatagacaat gtgaagtcga aaggagttac aacgtgagaa ttgctattcc
     1861 cctttccggc aagacgctgt cttcccattt tggacattgt gaacagtttg ccttcgtcga
     1921 agttgacgat caggcttggc aggtaaaggg tgtgactttc gttcagccgc cgcctcatca
     1981 gcctggtctt ctgccccgat ggctccacga gcagggagtg acaaccgtca ttgcgggcgg
     2041 catggggcct cgtgcccagc aactcttgag tgagctggga atcgatgtga ttttgggagc
     2101 aacgggatta cacccggaaa agcttgccgc cgattacctg gcaggtcagc ttgcaatcgg
     2161 tgaaaaccgt tgtagtcact gattacctgc gccgttgaca aaaagaggca gatcttaagg
     2221 cgttctcggg gccacctgcg acggctgttg ccgtttttgg gcctggacct ttcgggcggg
     2281 tttgtcaaag gggtcaggga tcccctccac aatcacatga gttcgcctgg cccaggcttc
     2341 atactgagcg gccatggcgg ccacccgttc aggggcttgt gccgacaaat cgtgcaattc
     2401 cgtgcgatcg gcctccatgt cgtacagttc ccatggttga cctgccaggg ccacgagttt
     2461 ccattttccg tcgcgcaccg cgcgattttg ctcgtggttg aaaaagaggg gctgcgaccg
     2521 cacgatgggc ttcccagaaa aggctggcac aaggctaacg ccttccatgg gttcgatggt
     2581 gtggccgttg tattgcgtgg ggtaagtagc cccggcaagc tgaatcaccg tgggcatcaa
     2641 atcaatcacg tgggcaggct ggtggtatag ttgccctcgc tgaccggcat ccaggcctgc
     2701 cggccaatgg acgatcaacg gcgtggcact gccgccctca tggacatagt gcttccactt
     2761 gcggaaaggc gtattgttga gcgtggccca gttctgcccc aaaaacacaa cagagtggcg
     2821 gtctcctgga ttgtcccctt cgcaacgacc gtttggcccg gactctgcgt ttccaccgtt
     2881 atcactgagg aagaagatca ccgtgttgtc gaggacgtcg cgctgtttta aacccgccac
     2941 caacgtgcca acactccggt ccatggcata aatcatggcg gcgtaaatgg ccatgatttg
     3001 gtcgaaccga tcccgctcgg cttcggaaac gctttcccac ggcggtgtat tctcgggaag
     3061 gggcgacaaa ggccaccgcg gatcgatcag ccccatttca atttgtcgcc gatatcgatc
     3121 ttcacgcagt ttatcccagc ccacaagata ctgcccacga aacttggcga tgtcggctga
     3181 tggggccatc aatgggaaat ggggggcaca atgtgctaaa taaaggaaaa acggcttttt
     3241 ggcatccagg gcctcgtcga tgaatttgag tccccattcc gtccacaggt cggcgccgta
     3301 ccagttgtct ccaaaaaggg gatcgtccgg ggccttttct tcaccattca aatagagctt
     3361 ggcttccttc cgccaggttt gcccagggaa atggacacct ccggcggcca ggttaagact
     3421 ccgcataaag ccccggctcc acggtggcgt accccgattt tgcccaaggt gccatttgcc
     3481 cgtcatggct gtgaagtatc ccgcttcctt caagacctcg gccatcgtga cactggaatc
     3541 agcgatgcgc gcttgatagc cgggatgatt ggggacgaac gtgccatcga gatgacccat
     3601 cccagcctga tggggataat ggcccgtgag aagtgatgcc cgtgtcggcg agcaacgccc
     3661 cgtattgtaa aactgcgtga aacgtaggcc attcttggcc agggagtcga gatgcggcgt
     3721 gggaatttcc gacccataac aacctatgtc ggaccacccc atatcatcca caagaatgat
     3781 gacgatattg gggcgctcac cgcccaaaat agactgcatc ggccccgcgc caagtcctaa
     3841 cgccaggacg ccgaagagct tgactcccca agcgcatact gccgacccac atcgcaaaaa
     3901 gaccatgtta tgcctcccac taaaaagcct tttcatcgta cgaaacgatg gccctattat
     3961 aagagctgtt tgatcagaca ttcaaggaat aaataggacg atcgcggcac ctccaactga
     4021 cgttaagcaa atccatggtg cgagggcaga cacaccgacc gaatgagctg atcccgggcc
     4081 aaagccacga agacgcaagc ccatgtttcg ggcaagctca tcgctcatat tggatttgca
     4141 tggtatcgaa gacctggcaa atccgagaaa cgatggcttg gatgtcagaa gatgccggtc
     4201 cacgcaggct ttccgccaaa cgcaagaggg cttcgcggga aactaggcca cgttgctggt
     4261 agtccatcaa agcccaggca gctgcccgcg ttgtgcggac aattcctcgg ccatgtcgtt
     4321 ttaaggcact ggcagggacg cattcgtgga cgatatttga tccgaaggcc gccagcagcg
     4381 cgaacgcctt gctcgcttgc gacacattgc cttcgatgac atgacgtgtc gtcaaaaaca
     4441 tggcaacatt ggcagtatat gccac
//
LOCUS       DAOSUT010000243         4393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8529_length_4393_cov_9.539419, whole genome shotgun sequence.
ACCESSION   DAOSUT010000243 DAOSUT010000000
VERSION     DAOSUT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8529_length_4393_cov_9.539419"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLD05_14990"
     CDS             <1..687
                     /locus_tag="PLD05_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78792.1"
                     /translation="ELRYQIRQFHSFIWASGGVYSDFYIHQIDECSWMKGAWPIKAHA
                     LGGRHYRGKAVDQNFDTYSIEYTYPDGTKLFYFGRQMAGCHNEFASYGHGTKGCCIIS
                     TSAHTPGRVRTFKGHDFAQENLLWAFPQPEPNPYQMEWVDLIEAIIKDQPYNEVERGV
                     QASLVTSMGRMAAHFGRIVTYEEALNHPHELAPDVDKFTEDSPAPVQFDETIGMYPQP
                     MPGIKTDREY"
     gene            complement(1349..2743)
                     /locus_tag="PLD05_14995"
     CDS             complement(1349..2743)
                     /locus_tag="PLD05_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78793.1"
                     /translation="MSSQGVPSLGATGTTARIDPPHRVGQTQAPQIATSAPRQDAGEP
                     GPVVVNEEIPISEHEEQDVERGVGLSPERIRRHARQIAAVLRRRQEQLDHREAQLNAQ
                     RAQLENDIRVLRLLWQERHAEWEEKRQLLAEREAALAERRAELGEREEALSQREQFLA
                     QWEEALRQREANLAKEEQALQEKRQELARREAEVQIRESDLTTFAVLVETAPAPPSVE
                     RSEGVPCERQADGVEDLIRKQDELTHYWQQAKRILSELEDARERWKEQEKQWRQQWVA
                     EQRQAMAELAAQREAVARRSQALDRLHAELLQLKKEVDARQREAIEHHLAACELTASL
                     ERNHAPAEVAQRLIALRQKLDQTVKMALEEREDRHQQTRTIETRLQSLHRRIVEEKRR
                     FDKYMAARLKELEDLAAKLADREKQLVLKESELHSQATAWQLAELGYILEIRRLRECL
                     QPDSQFGDLPESTT"
     gene            3197..4084
                     /locus_tag="PLD05_15000"
     CDS             3197..4084
                     /locus_tag="PLD05_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOP78794.1"
                     /translation="MVTEAFITLVFSRPRDPHAEPAFRHMVNEVLEAAGHLPAVIIPH
                     FYDAMPGGPVFTYLRRLTTDIILLAPLYPRAAQLVLRANGLRYRLARQTDDVPQRQNQ
                     SDRRLWAFDYREYGSFEVLRNAVAALVAEYLQGAGSLAGERPLVEEIEEATRSRWYPV
                     IDDEACVNCFECLNFCLFGVFDVNTEGRVFTAQPDQCRPGCPACARVCPAGAIVFPLY
                     PDPKIAGGDVVEEGQIIPLVDLKSGIGTVTTPQKPSVAGNSSQAPRPTGDQADGNEYH
                     PPTKQTLQQWLCQADDDAH"
     gene            complement(4114..>4393)
                     /locus_tag="PLD05_15005"
     CDS             complement(4114..>4393)
                     /locus_tag="PLD05_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78795.1"
                     /translation="LLREAGFHVGPTQSPITPIYADGLKAVYWARQLRERFAIWGAPS
                     TYPAVKMGQSLLRVIPTALHTPQDISYLVESLQMIEATLSLGQLMPVI"
BASE COUNT          869 a         1262 c         1233 g         1029 t
ORIGIN      
        1 gaattgcgct atcagattcg gcaatttcac agtttcattt gggccagcgg tggtgtgtac
       61 agcgacttct acatccacca gatcgatgag tgttcctgga tgaagggggc ttggccgatc
      121 aaggcgcatg ccttgggtgg acgacactat cgcgggaagg cggtggacca gaactttgat
      181 acgtattcca ttgaatacac ctatcctgac ggcaccaagt tgttctactt tgggcggcag
      241 atggctggtt gccacaacga gttcgccagc tacggtcacg ggacgaaagg ctgctgcatc
      301 atttccactt cagcccatac ccccggacgg gtccgcacat ttaagggtca cgattttgcc
      361 caggagaatc tcctctgggc tttcccccaa cccgagccca acccttatca aatggagtgg
      421 gtcgatctga ttgaggccat catcaaggat caaccctaca atgaagtgga acgcggtgtt
      481 caggcaagct tggttacgag catgggccgc atggctgccc actttggccg gatcgtgacc
      541 tacgaggaag cgctgaacca tccgcacgag ctggcccctg atgtcgataa gttcaccgag
      601 gactcgccgg caccagttca gtttgatgag acgatcggta tgtatcccca accgatgcct
      661 ggcatcaaga ccgatcggga gtattgatcc tcacggatct tggcaagacc cggcggtgga
      721 acacgatcgc ctttaaaaaa gcggttttac cgccagcaac ccttgaagat tgttctacca
      781 gacgaatcct tagctagaac ggaaggccca tagcggttat ccgctgtggg ccttcgcttt
      841 gcgctaagaa actttgggtg cgctggattt ggagagacgc gcgtccaatg tggtgccgac
      901 ggagatccta tcatgtcacg cggccacgac ggcgtgaagc cctcgaatga atcgggtggg
      961 cacacgtcct attcttgccc ggtgggtcgg agcgcctacc acggatcggc ccccacttta
     1021 cggtgcgggt tgtcgctttc cgattcagca atgtattgcg gccgcgctgt aaagattggt
     1081 aggggccaac gttgtgattg tccgttgttg agtctcagga gcgacacgcg gcgcgttgtg
     1141 ttaagagccc gtcccataat ctcccctcgc ccgctcggcg ggagaggggg gctttttgag
     1201 ataggctcta acccaaaacg acgcgcacca ttgcggccgc gggcgtgtga tactcatcct
     1261 aacaacgatt taccacgatg agaaacactc aagatgcctt gtgtgatacg acgagaagct
     1321 tgttccaacc cgttatacga ccaggaaact atgtagtact ctctggcaaa tcaccaaatt
     1381 gggagtcagg ttgtaagcac tcgcggagac gccggatttc caagatgtat cccaactccg
     1441 cgagttgcca cgctgtagcc tggctatgaa gctcgctttc cttgaggacg agttgctttt
     1501 ctcggtcggc aagtttggcc gcgaggtctt ctagctcctt aagccgggcc gccatgtatt
     1561 tgtcaaagcg ccgtttttct tccacaattc ggcgatgcag ggattgaaga cgggtctcaa
     1621 tcgttcgagt ttgctgatgc cgatcttccc gttcctccaa tgccatctta acagtctgat
     1681 ccagtttttg ccgcagggcg attaaccgct gggcaacttc ggcgggcgcg tgattccgtt
     1741 ccaaggacgc cgtaagctca caagccgcta ggtggtgttc aatggcctcg cgctgccggg
     1801 catccacttc ctttttgagt tgcaataact cggcgtggag ccgatcgagg gcctgtgaac
     1861 gccgcgcgac ggcttcgcgc tgggccgcga gttccgccat agcctgacgt tgctccgcta
     1921 cccattgctg acgccactgt ttttcttgct ctttccatcg ctctctggca tcctccaact
     1981 ccgacaggat tctcttggct tgctgccagt agtgcgtcag ttcatcctgc tttcggataa
     2041 ggtcttcgac cccgtcggcc tgacgttcac acggtacacc ttcgctgcgc tccaccgagg
     2101 gtggggctgg cgcagtctcc accaacacgg cgaacgtcgt taagtcgctt tctcggattt
     2161 ggacttcggc ctcgcggcgg gccaattcct gccgtttttc ctggagggcc tgctcctcct
     2221 ttgcgagatt ggcttcgcgc tggcgaagtg cctcttccca ttgtgcgagg aactgctcgc
     2281 gctgcgacag ggcttcctcg cgttcaccga gttcagcgcg gcgctccgca agggcggcct
     2341 ctcgctcggc caacaactgc cgtttttcct cccactcggc atggcgttcc tgccaaagga
     2401 gtcgcaggac acgaatatcg ttctcaagct gtgcccgctg cgcgttgagt tgcgcttccc
     2461 ggtggtcaag ctgctcttga cgccgccgga gaaccgccgc tatttgacgc gcatgccggc
     2521 ggattcgctc cggcgaaagt cccacccccc gttcaacgtc ttgctcctca tgttcactga
     2581 ttgggatctc ttcattaacg acaacaggtc cgggctcgcc agcgtcttgc ctcggcgccg
     2641 atgtcgcaat ctgtggagct tgggtctgac caactcggtg cggtgggtcg atccgtgcag
     2701 ttgtcccggt tgccccaagg gacggcacgc cttgcgatga cattgcatcg catgccgggg
     2761 ccatctgtgg gtgactcagc ggatcggaca ttgtcgggag actaaagatt cctgctctcg
     2821 gacgtcaagc tccatcacca ccgtggcgac gagcattgac cgcgacgacc aagttcaaag
     2881 gagcctcgcc gcagccttta tcgatataat cgacaaagtt tactcatttc ttgtattccg
     2941 tttctttgcg gggcaaatcg tgctccgcga acgctgcctc aaagctgtcc gcgctaccca
     3001 tcctgccaaa aagcggagat attacccatt ttgatgagct gtcaagaatg accaggtcaa
     3061 caatgatcaa ccagaccagt ttttccagat taccccgatt ctcggcaggg atcgcagtag
     3121 ccctttggaa gatcgacggt cctctcgcaa tttgctatac tgcgaataac tgattcgaat
     3181 ttcgtaaggt gtgattatgg ttactgaggc attcatcaca ttggtgtttt ctcgcccaag
     3241 ggatccgcat gcggagccgg cgttccggca tatggtcaac gaggtccttg aggcagccgg
     3301 ccacttgccc gcagtgatca ttccccattt ctatgatgcg atgccaggtg gcccggtgtt
     3361 tacatacctc cgccgactga caacggacat catcttgctc gccccgctgt atccccgggc
     3421 agcgcagctt gtgttgcgtg ccaacggtct gcggtatcgc ctggcccgtc aaaccgacga
     3481 cgtgccgcag cgacaaaatc aatctgatcg tcggctttgg gccttcgact accgggaata
     3541 tggctctttc gaagttcttc ggaacgccgt ggcggcactg gttgccgaat atctccaggg
     3601 tgcggggtcc cttgctggcg agcgaccgct agtggaggaa atcgaggaag ctactcgctc
     3661 acgttggtat ccggtcattg acgacgaggc atgcgtcaac tgttttgagt gtctcaattt
     3721 ctgtcttttc ggtgttttcg atgtcaatac ggaaggtcgc gtgtttaccg ctcaaccgga
     3781 tcaatgtcgt cccggttgcc cagcctgtgc ccgcgtttgt cccgccgggg ccattgtttt
     3841 tccactttat ccggatccca agattgctgg cggggatgtt gtcgaggagg gacaaatcat
     3901 ccctctagtg gatctgaaga gcggaattgg gacagtgacg accccgcaaa agccatcggt
     3961 ggcaggcaat tcctcacaag ccccgaggcc gacaggcgat caggccgacg ggaacgaata
     4021 tcatccccct acaaagcaaa cgctgcaaca gtggctttgc caagcggacg acgatgctca
     4081 ttagccctgc caagcggtac gcgggggtag tcatcagatg acgggcatga gttgaccgag
     4141 actcaatgtg gcctcgatca tctggagtga ttcaacaaga tagctgatgt cttgaggggt
     4201 gtggagtgcg gtcggaataa cccgcaagag tgactgtccc atcttcaccg cagggtaggt
     4261 ggagggggca ccccagatgg caaatcgctc ccgaagttgc ctggcccaat aaacggcctt
     4321 cagtccatcg gcgtaaattg gcgtgatggg cgactgcgtc ggtccaacgt ggaaacctgc
     4381 ctcccgaaga agg
//
LOCUS       DAOSUT010000244         4371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8579_length_4371_cov_10.932113, whole genome shotgun sequence.
ACCESSION   DAOSUT010000244 DAOSUT010000000
VERSION     DAOSUT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8579_length_4371_cov_10.932113"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..693)
                     /gene="infA"
                     /locus_tag="PLD05_15010"
     CDS             complement(469..693)
                     /gene="infA"
                     /locus_tag="PLD05_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOP78796.1"
                     /translation="MAKEKEKGIEVPGVVTEALANTNFRVQLDTGHTILAYVAGRMRK
                     NFIRIVPGDRVRVEISPYDPTKGRITYRER"
     gene            complement(904..1800)
                     /gene="folD"
                     /locus_tag="PLD05_15015"
     CDS             complement(904..1800)
                     /gene="folD"
                     /locus_tag="PLD05_15015"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOP78797.1"
                     /translation="MSAQLIDGKAIAQAIREEIRLGVERLRQEHGVVPGLATVLVGDN
                     PASVSYVTAKNKACAELGMASFQHTLPADITEEKLLALIDQLNRDASVHGILVQLPLP
                     KGICESRVIEAIDPRKDVDGFHPVNVGRLMLGDPVFPPCTPAGIQALIIRSGTPIEGA
                     EVVVVGRSNIVGKPVAVMLLQKQRGANATVTVCHTQTRDLAFHTRRADILIVAAGRPQ
                     AITGDMIRPGATVIDVGVNRVGYTESGKVKLVGDVDFDSAVKVAGKITPVPGGVGPMT
                     ITMLMRNTLRAARLHLGLPLDG"
     gene            complement(1938..2963)
                     /locus_tag="PLD05_15020"
     CDS             complement(1938..2963)
                     /locus_tag="PLD05_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78798.1"
                     /translation="MNCSPFSKLRSGEYGPLCGLVGRLRRGIIGMVLLALLAGCRNFP
                     SPQESYVGPQLPPNPTFEQIASFINTNSTQIRTVVADQASLSSPGFPTLRSQILFERP
                     WRLRIRGETSLSGLELDVGSNDELFWFWVKRNEPPAFYFCRYSDYDNPASTARRVVPI
                     DPRWLIEAVGITELDVNAPHEGPVPLAGGRLEIRTQRPTPDGIMTKVTVVDAQYGLIL
                     AQYLYDPQGRLVASAVSYDHRREPVTGLVLARTVEINVPASGMVLRLNLGNARINEPI
                     PNPSLTWNMPVYPGYPMVNLADPRNLSGISVVACPSRGTAQGPVAPFTGVEGGSGQGL
                     TWLPIPQ"
     gene            complement(3016..3573)
                     /locus_tag="PLD05_15025"
     CDS             complement(3016..3573)
                     /locus_tag="PLD05_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78799.1"
                     /translation="MIDFEVQRCTRHCAVTGRELKPGEVFYSVLIPDQQGWRRMDYSA
                     EAWHGPPDDCIAWWRTQLPTGSPKKRWAPSEVMIRWFEELADRADQADVRYVLALLMV
                     RRRILRLEGSRRANDGTEILTLFCPLNDTTYEIPAVIPSEDRLQQIEEQIAALMEGDE
                     IESLAGDGANSTPFRSEEGPKPERN"
     gene            4178..4250
                     /locus_tag="PLD05_15030"
     tRNA            4178..4250
                     /locus_tag="PLD05_15030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4211..4213,aa:Lys,seq:ttt)
BASE COUNT         1001 a         1313 c         1160 g          897 t
ORIGIN      
        1 gctaagaact catccgccga aatcgccgtc cgctcatcag cagggagatg agacacgttt
       61 tagctccacg agttcgccca cgacaaaccc gccagggaat cggaagacca tgacgcagta
      121 tagacacgca gtcggcgggt gtcaacgaac gccaaactgg tttttcacaa tcatcccaag
      181 gggaggagca aataggatgg acgagcacgg tacaccgtgg cccgcggtgg tgtgtccttt
      241 gcggctatgc ctctttctca ttaaacgaat ccatgcagag cggttcgccc ggaccaactt
      301 atccacagtc actgggtggc gtgataccga aagaaagtct tatacggaga cttaccgagg
      361 ctatacggcc ggcagaccaa cggtctttac gttcctggaa agagattcgg ccgtagcgcg
      421 aacaggccaa tagaccgagg cactagacaa aaacgagata gccaccgttt agcgctcacg
      481 gtaggtgatt cgccccttgg taggatcgta aggagaaatt tctacccgca cacgatcgcc
      541 cggcacaata cggataaagt ttttcctcat gcgtccggcc acataggcaa gaatggtgtg
      601 accggtatca agttgcactc gaaaattggt gttcgccaaa gcctccgtca caacacctgg
      661 aacttcgata cctttttcct tttccttagc cataaccacc ttccacccaa agacagcatc
      721 gaacaaattt cctaccgccc aaacgaccac cgcttgggag tgaaaacaga ggagggacac
      781 gttgctctca ttgtttcatc ctaccacgtg gacagcgtga ctccaagtgt ccaacggtcc
      841 ggccacgaca gggtgggcct ccattgagga attcctggag gcatgtgcac cgtggcgtcc
      901 ggttcatccg tccaatggca atcccaggtg taaccgcgcg gcacgaagcg tgtttcgcat
      961 caacatggta atcgtcatgg ggccaacacc gccgggaacg ggcgtgatct ttcccgccac
     1021 cttcacggcc gaatcgaaat ccacatctcc caccagcttg accttgccgg actctgtgta
     1081 acccactcgg ttcacaccaa cgtcaatcac cgtggcccca ggtcgaatca tatcgcccgt
     1141 aatcgcctgc ggccgtcccg ccgccacaat aaggatatca gcccgccgtg tgtggaatgc
     1201 caaatcccgc gtttgggtgt ggcacaccgt caccgtggca tttgccccac gttgcttctg
     1261 cagaagcatg actgcaacag gcttgccaac gatattactc cgccccacga cgacgacctc
     1321 cgccccctca atgggcgttc ccgatcgaat gattaacgcc tggatgcccg ccggtgtaca
     1381 tggtgggaaa acgggatcgc ccagcatcaa ccgcccaacg ttgacggggt gaaaaccatc
     1441 cacgtccttc cgaggatcaa tggcctcgat cacccgagat tcacaaatcc ccttgggcaa
     1501 aggcaactgg acaaggatgc cgtgaaccga ggcatctcga ttaagttgat cgatcaacgc
     1561 gagaagcttt tcctccgtaa tatccgccgg cagcgtatgt tgaaacgaag ccatgcccaa
     1621 ttcggcacac gccttgttct tggccgtgac gtacgacacc gaagccgggt tgtcacccac
     1681 caaaacggtg gccaatccgg gaacgactcc gtgttcctgc cgaaggcgtt ctactcccaa
     1741 gcgaatctcc tcacggatgg cttgagcaat tgctttgccg tcaatcaact gggccgacac
     1801 gatttccctc ctgatgctaa acaccaacct agggttaacc ggggaaggta tcctatgagc
     1861 cagacgacca cgcagataaa cgcttcccac acaccgacgg atccgggcat cttttagacg
     1921 ccaacagttc cattatccta ctgtggaatg ggaagccatg tcagcccttg gcccgaaccg
     1981 ccctccacgc cggtgaatgg ggcgaccggt ccctgagcgg taccacgaga agggcaagcg
     2041 acaacgctga tcccggacaa gtttcgcgga tcggcaaggt tgaccatcgg ataacccgga
     2101 taaaccggca tattccatgt cagagaagga ttggggatcg gttcgttaat gcgggcgtta
     2161 cccaggttaa ggcgaaggac catcccggac gccggcacat tgatctcgac cgttcgcgct
     2221 agcacaagcc ccgtcacggg ttcccgccgg tgatcgtaac tgacagcact tgccaccaat
     2281 cgcccctgag gatcatacaa atattgggcc aggatcaacc cgtattgggc atccaccacc
     2341 gttaccttgg tcataatacc atcgggtgtg ggccgctggg ttcggatttc caaccgccct
     2401 cctgccaacg ggacgggccc ctcgtgcggt gcattcacgt ccagctcggt gatgcccaca
     2461 gcttcgataa gccagcgggg gtcaatcgga acaacccgcc gcgccgtgct agcgggattg
     2521 tcataatcgc tgtaccggca aaaatagaag gccggtggct cgttgcgttt tacccaaaac
     2581 cagaaaagtt catcattgct ccctacatct agctccaagc cgctcaggct ggtttcacca
     2641 cgaatgcgaa gccgccacgg ccgctcaaag agaatttgag agcgaagtgt cggaaagccc
     2701 ggcgaagaga gactcgcttg gtccgccacc acagtccgaa tttgcgtgct gttcgtgttg
     2761 ataaacgagg caatttgttc aaacgtcgga ttgggaggca attgcgggcc gacataagat
     2821 tcttgcgggc tgggaaagtt tcggcagccg gcgagaagag ccaagagcac catcccgata
     2881 attccgcggc gcaaccgacc aaccagcccg caaagaggtc cgtactcacc ggatcgaagc
     2941 tttgaaaacg gcgagcaatt cataaagcca tccctggccg ctcttttatc caaaccggcc
     3001 gcttgaaggt ctttctcaat ttctctctgg cttcggccct tcctcgcttc ggaaaggagt
     3061 ggagttggcc ccatcaccag cgagtgattc gatctcgtcg ccctccatca aggccgctat
     3121 ttgttcctca atttgttgaa gccggtcttc cgagggtatc acggcgggaa tttcgtaagt
     3181 ggtatcattc aggggacaaa aaagcgtgag gatctcggtc ccgtcgttcg cccgccgtga
     3241 accctccagc cgcagaatcc ttcgtcggac catcagaagg gctaacacat aacgaacgtc
     3301 tgcctgatcc gcacggtcgg ccaactcctc gaaccaacgg atcatgactt cacttggtgc
     3361 ccatcgtttc ttcggactgc ccgtgggaag ttgggtccgc caccaggcga tgcaatcgtc
     3421 cggcggaccg tgccaagctt cagccgagta gtccatgcga cgccaaccct gctgatcagg
     3481 gatgaggacc gaataaaaca cctcacccgg cttgagctct cgccccgtca cagcacagtg
     3541 ccgcgtgcag cgttgaacct caaaatcaat cacggtctct tttccacagg tttttcgcca
     3601 acaaactctt tacacataaa cagaaaggct ttttttggaa tccgctcggc tttcggagta
     3661 acgttgccgt cacagccggt tttcctgcct ggtcgggtga caaggttagc gaaaagtgcc
     3721 cgggatttca accgcaaacg cccggctcga agagacacat tatttttcca tatattccca
     3781 aaaccctttt gtttcctaga ctcacgatag cgatgccaag ctgacgaagc ctacgtggag
     3841 gatcgaccag gcagccacag tagggcttcg ccgtccgggg ggctgacgat tgggtcgggc
     3901 ttccgctaga ataattaaga cgcgtaagaa agcacggcaa agccggtgaa cgccatggga
     3961 tcccatggcc ccaaaagggg ggtgggccgt cttaggcgga atcgcgaaaa ttggcgatta
     4021 tgaagtcaaa cgccgcgtgt cggcgcattt ccgggtcgcc ggtcactgcg cctcgacgcg
     4081 gtgggcgggc ggtaggttgc cggttgcttg ccggtcggcg ccgcgttcgg ctcgtggatg
     4141 cgtccacggt ctatggcatg agtgcagagt atcccacggg gctgtagctc agacggtaga
     4201 gcaacggact tttaatccgt aggtctcggg ttcgagtccc gacagcctca ctcaatctgg
     4261 caccagtttg gcaaacctcg cgcacggcgt gggggcttac gcgcgtcttc tcattctttt
     4321 gcgtctcgtc catcgcattc agctctggag gcgttcgcca cctctggttt c
//
LOCUS       DAOSUT010000245         4362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8593_length_4362_cov_10.520548, whole genome shotgun sequence.
ACCESSION   DAOSUT010000245 DAOSUT010000000
VERSION     DAOSUT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8593_length_4362_cov_10.520548"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLD05_15035"
     CDS             <1..290
                     /locus_tag="PLD05_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78800.1"
                     /translation="LAVVTGCPAGGTPKPKEYGIPMKTPLEEAKSLLENYAAGGSIGS
                     EAASYPDLVNAVRQSDPAKADILEKGFAELQKTPPQGVAAKAKEILAQLGQ"
     gene            complement(642..878)
                     /locus_tag="PLD05_15040"
     CDS             complement(642..878)
                     /locus_tag="PLD05_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78801.1"
                     /translation="MRRAGVQVLDPVLDHCQVEWLLVSADECPDQETEAWIIRTHFRI
                     PQTPWGQHKAYVKPVEVRRSRRWVLFRQVSGILD"
     gene            complement(1154..>1398)
                     /locus_tag="PLD05_15045"
     CDS             complement(1154..>1398)
                     /locus_tag="PLD05_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78802.1"
                     /translation="AVVLALAVGTMSNAFANSRQQYREAIRSMPLLERPNRFGHIYGN
                     TVRRIYHRRYGSLPGQQTTVPENGSHLVQPTPTPVQ"
     assembly_gap    1399..1498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1599..2987
                     /gene="glyA"
                     /locus_tag="PLD05_15050"
     CDS             1599..2987
                     /gene="glyA"
                     /locus_tag="PLD05_15050"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOP78803.1"
                     /translation="MLKSSPSDQVRRSSSPSMSVENTPTVEEVCSRRYPHLQAQDPEI
                     FRLVVAQSEHEYNTLKMIPSENYASFAVLEATGSILTNKYCEGYPAARYYEGNEFMDQ
                     IEQLAIDRLKALFGAEHANVQPYSGSPANQAVTRALVPPGCKIMGMPVPAGGHLTHGW
                     KVNFSGMDYEVVHYGPRVDTGMLDYDEIRDLARRERPRMIWVGATAYPRILDYSVFAE
                     IAQEVEAYLVADIAHINGLIVGGVHPNPVPYCDVVSSTCHKMLRGPRAGFILSRIEDR
                     WQHKYYPDSKLNLAKRIDRAVFPGLQGGPHMHVIAAMAVTFKEAATPQFREYARQVVA
                     NARRLAQRLMEKGYQLVSGGTDTHLLVMDFRDRPFTGKDVAQALAKAGIIANFNMVPG
                     DPRKPFVTSGVRLGTPALTTRGMKEPEMDRVAEWIDRVCQNLDRIDEIAPQIRAEIAE
                     LCRQFPPPGLLV"
     gene            complement(3071..4030)
                     /locus_tag="PLD05_15055"
     CDS             complement(3071..4030)
                     /locus_tag="PLD05_15055"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOP78804.1"
                     /translation="MIGRVFRRDFLRLAAIAGAKTLVGPSLFLRASEAASPQPPSEGL
                     TAYQEGPQIWVRWNNEILTSYRAHSTQKYPYFFPLTGPLSGLSLTTESSLPWPHHRSM
                     LFACDRVNRGNYWQGPVSLGQIVSQGPQLGQITPASVEILDECRWAPPNGDVVLSDRR
                     RFVVRLLSPTIRCIDADITWKAETNITIEPTNHSLFAVRAAPDITPLGGGHLVNAEGL
                     SGEKETFGKASSWCCYWGTRARTDIVEGIALFDHPQNPWAPTPWFTRDYGFISPTPFN
                     FIKQPWSMPGGTTLQLRYRVVLFGGSPEQAQLAALYEEWAKTS"
BASE COUNT          910 a         1160 c         1308 g          884 t
ORIGIN      
        1 ttttggcggt ggttaccggc tgccctgccg gtggcacccc gaagcccaag gaatacggca
       61 ttcccatgaa gaccccgctg gaggaggcta agagcctgtt ggagaattac gccgctggag
      121 gttcaatcgg tagtgaggcc gcaagctatc ctgacctcgt gaacgcagtt cggcagagcg
      181 accccgccaa agccgatatc ctggagaaag gcttcgccga attgcaaaag acaccgccgc
      241 aaggggtggc agccaaggcc aaggaaatct tggcacaact gggccaatag ttagaagggc
      301 ctatctcaaa gtttcctctc cctctgggag agggttaggg tgagggcaag cgacggtcgc
      361 agtcgatcga gaaaacgggc agcgattcga gtcaccctca cccccggccc ctctcccgcc
      421 gaacgggaga ggcgaggtcg ttggacaggg cctaagaacg cccaaaacgt ttttctgtcc
      481 gtgcggcatt tgttaggccg aggattgacc cccgccggta atcttcggcc ttttgtgtgt
      541 ttcttgtgct gcaaattctt gcgcagcaat tgagacaact cggcaaaccg accgaggaaa
      601 ggcggacgac gccgctggtt ttgttcgtcc tagggttgat cctaatcgag gatgccggag
      661 acctggcgga agaggaccca acgtcgtgag cgacggactt ccaccggttt gacgtaggcc
      721 ttatgctgtc cccagggggt ctgggggatg cggaaatggg tgcggataat ccatgcttcg
      781 gtttcttggt ccgggcattc gtccgccgag accagtaacc actcgacctg gcagtgatcc
      841 aacaccgggt ccaagacctg cacaccagct cgtcgcatcg ctttcactcc ttcttccatg
      901 ggacgggatc ggtggtgagc ctcaatggca gaagaagctc aagcgaatgc cgtaccaatt
      961 gaggtgatcc tgacgggaca gccaagaccc gcggccaaga ccgcctcaat cccaaggacc
     1021 agagcgagct ggagatgtca tttcacgtca gcgacgtgtc ctctgcgttg cttgacggca
     1081 acacaacagc ggggcgcgcc aagcggcttg tccgtaacgc ctgtgaacca ggaaagtaac
     1141 ccgtgaggga tggctattgc acgggggtcg gcgtgggttg caccagatgg gagccgtttt
     1201 cggggacggt cgtttgctga ccgggcaggg aaccataccg tcggtggtaa atccgccgaa
     1261 cggtgttgcc gtagatgtgg ccaaagcggt taggccgctc caaaagcggc atcgagcgga
     1321 tggcctctcg gtactgttgt cggctatttg cgaaggcgtt gctcattgtg ccaacggcca
     1381 aggccaggac aacggcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1501 gcggggtgtg tgcaggcttc gcgcgaattg agcccggccc caaggcgtcc gaggtcgccc
     1561 gataccctaa ccgatctgtt ccaacgtgag gtttttcgat gctgaagtcc tccccctcag
     1621 atcaagtccg acgcagttca tcgccttcca tgtcggtgga aaacaccccg accgttgagg
     1681 aggtctgctc acggagatat ccccatcttc aggcccagga cccagagatt ttccgcctcg
     1741 tggtggccca gtccgagcac gagtataaca cgctaaagat gataccgtcg gagaattatg
     1801 catcttttgc ggttctggaa gccacggggt cgatcctcac caacaagtat tgcgagggct
     1861 atcctgccgc gcgttactac gagggcaacg aattcatgga ccagatcgag caactggcca
     1921 tcgaccgatt gaaggccctc ttcggtgcgg agcacgctaa tgttcagccg tactccgggt
     1981 cgcctgcaaa ccaggctgtg actcgcgcgc tggtcccgcc gggctgcaaa atcatgggta
     2041 tgcccgttcc cgctggggga cacttgaccc atgggtggaa ggtcaatttt tcgggcatgg
     2101 attacgaggt ggtccattac gggccgcggg tcgatacggg aatgctcgac tacgacgaga
     2161 tccgggattt ggcgcgacgg gaacgcccac ggatgatttg ggtgggtgcc acggcctatc
     2221 cgcggattct cgattacagt gttttcgccg agattgccca ggaggtggag gcctacctgg
     2281 tggccgacat cgcccatatc aacggtttga tcgtgggagg tgttcacccc aacccggttc
     2341 cgtattgcga tgttgtctca agtacgtgcc acaagatgct gcggggaccg cgggcggggt
     2401 ttatccttag ccgcatcgaa gaccgatggc aacacaagta ctatcccgac agcaaactca
     2461 atctggccaa gcggattgat cgggcggttt ttcccggctt gcaaggtggt ccccacatgc
     2521 acgtgattgc tgctatggcg gtgacgttta aggaggccgc cacgccgcaa ttccgggagt
     2581 atgcgcgaca ggtggtggct aatgcccgac gcctcgctca gcgactgatg gaaaaaggtt
     2641 atcaacttgt cagcggtggc acggacacgc acctcttggt gatggatttt cgggaccggc
     2701 cattcacggg caaggacgtt gcccaggccc tggccaaggc gggaattatt gccaacttca
     2761 acatggtgcc gggcgatcca cgcaagccat ttgtcaccag tggtgtaagg ctgggaacac
     2821 cggccctcac gacccgcgga atgaaggaac cggaaatgga ccgcgtggcc gaatggattg
     2881 accgcgtgtg ccaaaacctc gatcggatcg acgaaattgc cccgcagatt cgcgcggaaa
     2941 ttgccgagct gtgccgccag tttccgcccc cggggttatt ggtttgattt gcggcgcaac
     3001 cggataaacg cggcttcacc ccgtgtcggg accgtcagga aatttagctc ggctctctat
     3061 tccgcctagt ttatgatgtc ttggcccact cttcgtagag ggctgccagt tgggcctgct
     3121 cgggactccc gccgaagagg accacgcgat agcgaagctg aagggtcgtc cctccgggca
     3181 tggaccacgg ctgtttgatg aaattaaatg gcgtgggcga gataaagccg taatcacgcg
     3241 tgaaccatgg ggtcggtgcc caggggtttt ggggatgatc gaagagggcg atcccctcga
     3301 cgatgtcggt tctcgcccgc gtcccccaat agcaacacca actcgacgct ttaccgaagg
     3361 tctccttttc tccgctcagc ccttcggcat tgacgaggtg accgccgccc agtggcgtga
     3421 tgtccggcgc tgcccgcacg gcaaacaacg agtggttggt tggctcaatg gtgatgttgg
     3481 tttctgcctt ccatgtaatg tcggcatcga tgcaacgaat ggtcggggag aggagacgaa
     3541 ccacgaagcg acggcgatcg ctgagcacga catcaccatt gggcggcgcc cagcggcatt
     3601 catcgaggat ttctacgctt gccggcgtga tctggcccaa ttgcggtcct tgggaaacga
     3661 tctgtcctaa gctgaccggt ccctgccagt aattgccgcg attgacgcgg tcacaggcaa
     3721 agagcatgga acgatgatgc ggccaaggga gtgaactctc cgtagtcagg gagaggccag
     3781 agagtgggcc agtcagcggg aaaaaataag gatacttctg tgtggaatgg gcgcgatagc
     3841 tggtgaggat ttcattgttc cagcgaaccc agatttgggg accttcctga taggctgtca
     3901 agccctcact gggaggctga ggcgatgcgg cctcactggc gcgaaggaac agcgaaggcc
     3961 cgaccagggt tttcgcgcca gcaatggcag ccagtcgaag aaaatcacga cgaaaaactc
     4021 ggccgatcat gggagctgtc tcctcaaaga aggtcactca cgttcgaaaa ccatgtgaca
     4081 tcatatccag aacggacacg cgtttccagg tatgtcaatt accccgatgt ttttcttcag
     4141 aaatccgaga catcgatacc ctgtgaatga acgtggtgca cattttgcta gatatcgcaa
     4201 ctcatcttgt ttgtgggtag caagtgacaa aatgcttgac gtattgggtc cacggcgcta
     4261 tactggaaat gtaatcgggt agctctagtc ctgggctatt tctgttggga atggttggtt
     4321 gatgtcttgc aatgtggagg acttagttat ggaacgtcga gg
//
LOCUS       DAOSUT010000246         4361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8595_length_4361_cov_9.738737, whole genome shotgun sequence.
ACCESSION   DAOSUT010000246 DAOSUT010000000
VERSION     DAOSUT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8595_length_4361_cov_9.738737"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..623
                     /locus_tag="PLD05_15060"
     CDS             114..623
                     /locus_tag="PLD05_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78805.1"
                     /translation="MQIPATFLRGLFSGVVGQPTRPVERNDQTKPQSADDKTLSDSLM
                     TVLGQSGNPSLWEILKEYDVTHITPRQFSQMLQQLRESGSLTEQEYTELAQIRRDLAE
                     AGVDADEPVDLIDFYQKFIRKLQLMQALGTAESTDDLQTALQRAEKHLKWLEKISTLQ
                     TELGTDAWA"
     gene            complement(748..2511)
                     /locus_tag="PLD05_15065"
     CDS             complement(748..2511)
                     /locus_tag="PLD05_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOP78806.1"
                     /translation="MMAEFKENGLSETNVTKPQGSEKTRCCAENAQGTDFIREAIIED
                     LRTGRYDRVHTRFPPEPNGYLHIGHAKSICLNFGIAQEFGGKCNLRFDDTNPTKEDVE
                     YVEAIKEDIRWLGFDWEDRCYYASDYFEQLYQWAEELIKKGKAYVCDLSPDEVREYRG
                     TLTEPGRESPYRNRSVEENLDLFRRMRAGEFPDGSRTLRAKIDMAHPNLNMRDPVMYR
                     IIRATHHRTGDKWCIYPTYDWAHGQSDSIEGITHSICTLEFENHRPLYEWFIKELGIF
                     APRQIEFARLNLTYTVMSKRRLLQLVQEGVVRGWDDPRMPTICGLRRRGYTPAAIRDF
                     CQRIGVSKYDGVVDISLLEHCIREDLNKHAPRVMAVLRPLKLVIDNYPEGQVEYLEAV
                     NNPEDPSMGTRQVPFSKVLYIEQDDFREVPPPKYYRLAPGREVRLRWGYFVRCTHVVK
                     DPQTGEVIEVHCTYDPETRGGNAPDGRKVKATIHWVSAMHCIEAEVRLYDYLFTKPDP
                     DDVPEGLDWRANLNPNSLEIIPNAKLERSLAGAPVGSRYQFERLGYFCVDPDSTPEKL
                     VFNRTVTLKDTWAKIQQKMGM"
     gene            complement(2597..4174)
                     /gene="gltX"
                     /locus_tag="PLD05_15070"
     CDS             complement(2597..4174)
                     /gene="gltX"
                     /locus_tag="PLD05_15070"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOP78807.1"
                     /translation="MTVRTRFAPSPTGYLHIGGVRTALFCWLFARRHGGQFILRIDDT
                     DQQRNVEEALRPILDGLRWLGIDWDEGPEVGGPCQPYFQSRRLPIYQEAVRQLLSTGH
                     AYWDFATPEEIQAEREAAQREKRAFVYSRRFMAETEAEAARFRAQGRTGVVRLKMPRE
                     GKLIIDDLIRGRVEFDWAQEQDHVIQRSDGTVLYHLASAVDDHAFGITHVIRAEEHLS
                     NTPRQVFILRALGHEPPQFAHLPFVAEPGSKVKLSKRKLDKYLKNRDFAELMELGQKI
                     AEKIRYSAVSELFNPVVVDFYKVVGFLPEAIVNYLALLGWSLDDRTEHFTRNELIQAF
                     SLERVNKAPASFDPKKLMAFQQRHMKELPLDEKVSMVIPYLQQAGWIEEPISPPLRQR
                     IADVVTAAGDRLRVAGEILNYPEFFLSDDQINYDPKDVQKRLSGTEVGPRLEALASLF
                     ETLETFTPESLESAIHRFAESQAIALGEVIHPLRVAVTGRSVGLGLFETLAILGRETV
                     CRRLRLARQRFCQPATQ"
BASE COUNT          957 a         1256 c         1161 g          987 t
ORIGIN      
        1 gcgcgtcgat cgtggtttga aaaaaacagt ggtacggcgt ttgcacatcc cggcggatca
       61 tgtctgccgc caatatgcgg caaatgtcct ttttttgatg gagagatgac gcgatgcaaa
      121 tcccagccac ctttttgcga ggtttgtttt cgggtgtcgt tggtcagccc acacgtcccg
      181 tggagcgaaa cgaccagacc aaaccgcagt cggccgacga caaaaccctc agtgacagct
      241 tgatgacggt gctcggtcag tcgggcaatc catctttgtg ggaaatcctc aaggagtacg
      301 atgtaaccca catcactccc cggcaatttt cgcagatgct ccagcaactg cgagaatctg
      361 gttccctcac cgagcaggaa tacaccgagt tggcacaaat ccggcgtgat ctggcggagg
      421 ccggtgtcga tgcggacgaa cctgtcgatc tcatcgattt ttaccagaag ttcattcgca
      481 aattgcagct tatgcaggct ttagggactg ccgaatcaac agacgatctc caaaccgctt
      541 tgcaacgcgc ggaaaagcac ctgaaatggt tggagaagat ctcgaccctt cagactgagc
      601 tgggaaccga tgcctgggcg taggccttga gccccggtgc acttggcttt gtgggggccg
      661 cgcaaatcac cgccatgaag caagccgaga gccccaccat gtgcggcggc cgcttctttc
      721 tctgcggctt gcctgacgca cctcgtgtca cattcccatt ttctgctgga ttttggccca
      781 ggtgtccttg agggtgacag tccgattgaa gaccagtttc tctggcgtac tgtccggatc
      841 gacacagaaa taccccaatc gctcgaactg ataccgtgac cccacagggg cccccgcaag
      901 gctccgctcc agttttgcgt tgggaatgat ctctagcgag ttgggattca aattggcccg
      961 ccaatcgaga ccttcgggca cgtcatcggg atcgggcttg gtaaagaggt aatcgtacag
     1021 acggacttcc gcctcgatgc aatgcatggc agaaacccaa tgaatggtgg ccttgacctt
     1081 tcggccatct ggggcatttc ccccgcgggt ctcgggatcg taggtgcagt ggacctcgat
     1141 cacctctcct gtttgtgggt ctttcacgac gtgcgtgcag cggacaaaat agccccaccg
     1201 gagtcgcact tcccgccccg gcgctaaacg atagtacttc ggcgggggaa cctcgcggaa
     1261 gtcatcttgt tcgatgtaga ggaccttaga aaacggcacc tggcgcgtac ccatggaagg
     1321 atcctcaggg ttgttgaccg cctcgagata ttcgacttgg ccttccggat agttgtcgat
     1381 gacgagcttc agaggtcgta ggacggccat cacccgcggg gcatgcttgt taagatcttc
     1441 ccgaatgcaa tgctcgagta agctgatatc gacaaccccg tcgtatttcg aaacgccaat
     1501 cctctggcaa aaatcccgaa ttgctgccgg agtgtaaccg cgacgccgca ggccacaaat
     1561 cgtgggcatc cgcggatcgt cccagcctcg gacaacccct tcctggacaa gctggaggag
     1621 tcggcgctta ctcatgaccg tataggtcaa gttcaggcgg gcaaactcga tttggcgcgg
     1681 agcgaagatg cccaattctt taataaacca ctcatacaag ggccgatgat tttcgaactc
     1741 cagtgtgcaa atcgaatgcg tgattccttc aattgaatcc gactgaccat gggcccagtc
     1801 atacgtcggg taaatgcacc atttatcacc cgtgcgatgg tgcgttgccc ggatgatgcg
     1861 gtacatcaca gggtcgcgca tgttcaggtt ggggtgggcc atgtcaatct tggcgcgaag
     1921 ggtccgcgac ccgtcgggaa actcccccgc ccgcatgcga cggaaaaggt ccagattctc
     1981 ttccaccgag cggttgcgat acggactctc tcgcccgggt tccgtgagcg tcccccgata
     2041 ttcgcggact tcgtcgggcg acagatcaca gacgtaagcc ttgccttttt tgattagttc
     2101 ctctgcccat tgataaagct gctcaaaata gtccgatgcg taataacagc gatcttccca
     2161 atcgaaccct aaccagcgga tgtcttcttt gatagcttcg acatattcca cgtcctcttt
     2221 ggtggggttc gtatcgtcaa aacggagatt gcacttgcca ccgaattcct gagcaatacc
     2281 aaagttaagg caaatcgact tggcatggcc aatatggaga tacccgttcg gttccggtgg
     2341 gaaccgcgtg tggacacgat cgtaccgacc ggtccgcagg tcctcgatga tggcttcccg
     2401 aataaagtcc gttccctgcg cgttctcggc acaacagcga gtcttctctg acccctgcgg
     2461 tttggtcaca tttgtttccg acaatccatt ttccttaaat tcggccatca tacgatgact
     2521 ccttattgac ttcccagcta aagtgcctct taccaattct cattcgtgga ccatacagtg
     2581 ctgccgactt ctcgcatcat tgtgtcgccg gttgacaaaa acgctgtcgg gccaatctca
     2641 gcctgcggca gacggtttct cggccaagga tggccagcgt ctcaaatagt cctagtccca
     2701 cactgcgacc cgtaacagcc acacgcaacg ggtgaatcac ctcgcccaag gcaattgcct
     2761 gcgattcggc aaaccggtga atggcggact ccagcgattc gggagtgaat gtctcgagtg
     2821 tttcgaacaa cgaggccagg gcctccaacc tcggtcctac ctctgttccg gatagtcgct
     2881 tttggacgtc tttaggatca tagttgattt ggtcatcact gagaaagaat tctggataat
     2941 taaggatttc tcccgcgact cgtaaccggt cccccgccgc cgtcacaacg tcggcaatgc
     3001 gttggcgcag aggcggcgag ataggttcct cgatccatcc cgcctgctgg aggtagggta
     3061 tgaccatgga taccttttcg tccagtggta actccttcat gtggcgctgc tggaaggcca
     3121 tcagtttttt gggatcgaag ctcgccggcg ccttattgac ccgctccaag gaaaatgcct
     3181 ggatcaactc atttctcgtg aagtgctcgg tccgatcatc cagggaccaa ccgaggaggg
     3241 ccaagtagtt gacaatggcc tccggcagga agcccaccac cttatagaag tcgacaacca
     3301 ctggattgaa aagttcgctg acggcagaat agcggatttt ctcggcgatc ttttgcccaa
     3361 gctccatcaa ctcggcgaaa tcgcggttct tcaggtattt atccagtttt cgcttgctta
     3421 atttgacttt gctcccgggt tccgccacaa acggcaggtg ggcaaattgc ggtggctcgt
     3481 gccccaacgc ccgcaagatg aacacctgcc gaggggtatt ggaaagatgc tcttccgccc
     3541 ggatgacgtg cgtgattcca aaggcatgat catccacggc gctggccaag tgatagagga
     3601 cagttccgtc cgaccgttgg atcacgtggt cttgttcctg ggcccaatcg aactcaaccc
     3661 gcccgcgaat gagatcatca attatgagtt tcccttcccg gggcattttg agccgcacga
     3721 caccagtgcg accctgagcg cggaatcggg cggcctcggc ctccgtttcg gccataaacc
     3781 ggcggctata gacgaaggcc cgcttttccc tttgcgcggc ctcccgctcg gcctgaatct
     3841 cttccggcgt ggcgaaatcc caataggcat gacccgtact caaaagctgg cgaaccgctt
     3901 cctgataaat ggggagccgc cgcgactgaa aatagggttg acacgggcca ccaacctctg
     3961 gtccttcgtc ccaatcgata ccaagccagc gcagcccgtc caggattggc cgcaacgcct
     4021 cctcgacgtt ccgttgttga tccgtatcgt caattcgcag gataaactgt cccccatggc
     4081 gtcgggcaaa tagccagcaa aacagggcag tcctcactcc gccaatatgg agataacccg
     4141 tgggactcgg tgcaaaacgt gtacgaaccg tcataagaac gtccgatgcg agatgatttg
     4201 aatacactcc atcgacccat caaccaacgg ttttggtcca atcctttctc cctggggaga
     4261 tgggaccacc ttagcggtgg tcagcggggc catggaaaac cggtgaaaga aattgagtat
     4321 atcggccgac gggttttttg gctaccagaa acggcttcga c
//
LOCUS       DAOSUT010000247         4333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8649_length_4333_cov_6.286115, whole genome shotgun sequence.
ACCESSION   DAOSUT010000247 DAOSUT010000000
VERSION     DAOSUT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_4333_cov_6.286115"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLD05_15075"
     CDS             complement(<1..883)
                     /locus_tag="PLD05_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOP78808.1"
                     /translation="MGKNGSATNLRGHEYEEADFRAAMPAPKEGIFLPGTMIEVIRVP
                     TSPGTYRHVLFDFDGTLSLIREGWPDVMIPMMVECLLETGTQETEEELEDTVRAFVME
                     LTGKQTIYQMIRLAEEIKKRGGRPLDPTEYKRMYHDRLMARIAHRREGLRSGQIRPEE
                     MLVPGSLELLEELQRRGVQLYLASGTDEVYVREEATLLGLDRYFGEHIYGAIDDYKRF
                     SKAMVIEQILATHRITGNLLLGFGDGYVEIQNVKEVGGTAVAVASDEAKKSGQPDPWK
                     RERLISVGADLVIPDYRD"
     gene            complement(982..1632)
                     /locus_tag="PLD05_15080"
     CDS             complement(982..1632)
                     /locus_tag="PLD05_15080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOP78809.1"
                     /translation="MPKIVDLTLPMLDGGPSYPKDPQLRLKDFRKLPEDGCNVTEITM
                     GSHQGTHLDAPSHFLQNGKSLDQIPLERFYGPAVLVDLAPDGELPPKTPITPDMLQPF
                     APLFREGHRVLCRTGWYNHFSQPEYFTDLPSLTAEAARWIAARRISLLGIDMPTPSKI
                     AGRECHEVLLGPGAEIVLVEGLTNLHLLPREFIFVGFPLNLPGRDGSPIRAVAIIE"
     gene            2318..4120
                     /locus_tag="PLD05_15085"
     CDS             2318..4120
                     /locus_tag="PLD05_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOP78810.1"
                     /translation="MASISGFSAPSLVDTESLPRLLGGRDSPGIPEKSEASRGAGAQL
                     LEALEESFGTAFSVYDGVSGELQTANQELRIDWELFGILCRVVSQRRQPEIVWEEDPF
                     IFLAVPYQQSADEERVAVGAFVSRFVGLDEDLSLQAKSLSGDPQRIRDWAIRQTPWQP
                     EALLRVATASLRQLRQRTELALLEKQMEEMSVHMAAVYEEISLLHRLTRDLRLSKSDE
                     ALAETAATWLLEIVPAEGIGAVFLPVAEQDDNFLGNIRTKSVLLTAGDFPFSADTFLA
                     LADHFQVRSSKDPVVKNFVEAPFHFAGVQLRNLIAVPILEGENLFGYLAAVNHVQGGQ
                     FGSMEANLLHSVATIIGVHSGNIDLYRQQAELLAGVVKALTSAIDAKDPYTCGHSDRV
                     ARIAVRLAQELGYRPETLSRVYLAGLLHDIGKIGVNDAVLQKPGKLTDQEFELIKTHT
                     KTGFRILSGLKQLEDINKVVLHHHEAWNGSGYPEKLVGEAIPQIARIVAVADAYDAMA
                     SDRPYRTRMPNDKIDEIIRAGAGRQWDPEVVEAFFRARDDLREIADEKDLKFLNHPLL
                     GPMIRKSLGLPENGNGNSTTAGSQAESEASKRAP"
     gene            complement(4084..>4333)
                     /locus_tag="PLD05_15090"
     CDS             complement(4084..>4333)
                     /locus_tag="PLD05_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78811.1"
                     /translation="RSDLRDFVNVGSATAPETFRFADNWWYCEDRPAASKPRLPVQET
                     GGIYGKDPQLRETQGGWWKAENPAAAAYGARLEASDSA"
BASE COUNT          916 a         1201 c         1225 g          991 t
ORIGIN      
        1 aatcccggta gtctggaatt acgagatcgg ccccgacgct gatcaaacgt tcgcgtttcc
       61 agggatcggg ttggccactc tttttggctt cgtcactggc cacggccacg gcggttccgc
      121 ccacttcttt cacattctga atctcgacgt atccatcgcc aaatccgagc aggagattgc
      181 ccgtaatccg gtgtgtcgcc aagatttgtt caatgaccat ggccttggaa aatcgtttgt
      241 aatcgtcaat ggctccataa atgtgctcac cgaaataccg gtccagtccc aacagggtcg
      301 cttcttcacg gacataaact tcgtctgtac cgcttgccaa atagagctgc accccgcgcc
      361 gctgcaattc ttccagcagc tcaagggagc cggggacaag catttcttca ggccggattt
      421 gtcccgaacg taaaccctcc cgccggtgag cgatccgcgc catgagccga tcgtgataca
      481 tccgcttata ctcggtcgga tcgagcggcc ttcccccacg ctttttgatt tcttccgcca
      541 agcggatcat ttggtaaatc gtttgttttc ctgttaactc catgacaaag gcacgtaccg
      601 tgtcttccag ttcctcttct gtctcttgag tacccgtttc gaggaggcat tccaccatca
      661 tggggatcat gacgtcgggc cagccttccc ggatcaaact cagcgtaccg tcaaaatcaa
      721 agaggacatg acgatatgtg ccgggcgacg tcggaacccg aatgacttca atcatagtcc
      781 ccggcagaaa aatcccttcc ttcggggctg gcatggccgc gcgaaaatcg gcttcctcgt
      841 actcgtgtcc ccgcaaattc gtggcgctac catttttgcc cataggggat gtcatatctg
      901 tttcggtaat tctctggttc aagccacacc gggtgattct cgcactattg cactatgtcg
      961 tgtgcagtac acgaaagtga actactcgat gatggccacc gctcggatag gggacccgtc
     1021 tcggccggga aggttgagcg gaaaacccac aaagatgaat tctcgtggca gaagatggag
     1081 gtttgtcaaa ccttccacaa ggacgatctc cgcgcccggt cctaacaaca cttcatggca
     1141 ttctcgaccg gcgattttac tgggcgtcgg catgtcaatc ccgagcaggc tgatccgtcg
     1201 ggcggcgatc cagcgggcgg cctcggcggt cagagatggt agatcggtaa aatattccgg
     1261 ctgactaaag tggttgtacc accccgtccg gcaaagtacc cgatgcccct cacgaaacag
     1321 cggggcaaaa ggttgaagca tgtcgggggt gatcggcgtc ttgggaggaa gttcaccgtc
     1381 gggggccagg tccacgagaa cagccggacc gtagaatcgc tccagaggaa tctgatccaa
     1441 cgactttccg ttttgtagga aatgagatgg ggcgtcaaga tgtgtcccct ggtgcgagcc
     1501 catcgtgatt tccgtgacgt tacacccatc ctccgggagt ttgcgaaaat ctttgaggcg
     1561 aagctgagga tccttcggat agctcggacc gccatccagc ataggtagtg tcaaatccac
     1621 aatcttcggc atggtcgtta tctcctaaca aagggcaaag cagatcaagc cgagtggtct
     1681 gccttaaagg tcaaatcagt ctcgggcgga aaggtactgt cgcaaacggt cattctcgga
     1741 aatgagatga tcccttggca agaggcgagg gacttctgcg gatcgggtcg atctggccgt
     1801 aacctgccgc cgatttggtg gcatcggtcg tttgaatggg cgggaagatt tctggggata
     1861 tcgggcttga ccgcagcgcg ggcaaatgta tatgatattt tagacatgcc aattaggcat
     1921 aaatgatggt tgtggtgcag atggttcaaa gaacgaggtc ggtggcttga gttcagagag
     1981 aaatattcgg aggaggccga tttaatgcga tcatgctcgg gagtaaccgg gtgagctgaa
     2041 acactccctg tccttggatt ccccgttccc ctttgtccct cccggaagcg gccgcgtccc
     2101 gaacccatgg aatgatcggg acgcggcttc gttttataga taccgcgcac cggtctggtt
     2161 ggggtgtccc caatcttcct agcttgccta tcttttgatc tttcacaaaa agtgttctag
     2221 agtttcccta gggaaccgcc gatagttcgt taacattggc ctgcttaatg gggcccggta
     2281 ggcgtcggga tctgtttttt tgggatggga atctgacatg gcgtcgatct ccggtttctc
     2341 ggctcctagc ctggttgaca cggaatcgtt accccgtctc ctgggtggaa gagattcccc
     2401 tggtattccg gagaaatcgg aggcgtcacg cggggccggc gcacagctct tggaggccct
     2461 tgaagagtct ttcggtacgg ctttttcggt ttacgacggg gtttcgggcg agctccagac
     2521 tgccaatcaa gagctacgga tcgactggga gttattcggt atcctttgcc gcgttgtgtc
     2581 gcagcgacgc cagccggaaa tcgtctggga agaagaccct ttcattttcc tggccgttcc
     2641 ctatcaacag tcggccgatg aggagcgggt ggctgttggg gcctttgttt cccgatttgt
     2701 gggcttggac gaggacctct cccttcaagc aaagagtttg tcgggggatc cgcagcgaat
     2761 tcgcgattgg gccattcgac agacgccttg gcaaccggag gccttgctcc gggtcgccac
     2821 ggccagcctg aggcagctcc gtcaacggac ggaattggcc ctgttggaaa agcagatgga
     2881 ggagatgtcg gtccacatgg ccgcggtgta tgaggagata agtctcctcc ataggctgac
     2941 acgggaccta cggttgtcaa aaagcgacga agcccttgct gagaccgccg cgacctggct
     3001 gctggaaatt gtacccgccg agggaatcgg cgcagtgttt ttgcctgtcg ccgaacaaga
     3061 cgacaacttt ctgggaaata ttcgcaccaa gtcggtgtta ctcacggccg gggatttccc
     3121 gttcagtgcg gacaccttcc tcgcgttggc cgatcatttt caggtccgtt cctctaagga
     3181 ccctgttgtt aagaactttg tggaagcgcc attccacttc gccggggtcc agctacgaaa
     3241 cttgattgcc gtgccgatcc ttgagggcga gaaccttttt gggtatctgg ccgccgttaa
     3301 tcacgtccaa gggggacagt ttggaagcat ggaagcgaac ctcttgcatt ccgtggccac
     3361 gattattgga gttcacagcg gcaacatcga tttataccgg cagcaggccg agcttcttgc
     3421 aggtgtggtg aaggcgttga cctccgccat tgacgccaag gatccctaca catgcggtca
     3481 cagtgaccgt gtggctcgca ttgccgtccg gttggcccag gagcttggtt accgtccgga
     3541 gacactgtcg cgagtctatc tggcaggtct tctgcacgac attggtaaga tcggcgttaa
     3601 cgatgccgtc ctccaaaagc ccggcaaatt gaccgaccag gaatttgagc tgatcaagac
     3661 ccacacaaaa accggcttcc gcattctctc agggctaaag caactcgagg acattaacaa
     3721 ggttgttctc caccaccacg aagcctggaa cggcagtggt tatccggaaa agctcgtcgg
     3781 cgaggccatt ccccagattg cccgaattgt ggccgtggcg gacgcctacg acgccatggc
     3841 cagtgaccgt ccttaccgaa cccggatgcc caacgacaag atcgacgaaa tcatccgggc
     3901 gggggctggt cgccagtggg accctgaagt ggtcgaagcc tttttccgag cgcgcgacga
     3961 tcttcgtgag atcgccgacg aaaaggacct aaagttcctc aatcatcctc tcctggggcc
     4021 gatgatccga aagtccctcg gcctgccgga aaacggaaat ggaaacagca cgaccgctgg
     4081 gagtcaagcc gagtctgaag cctccaagcg cgccccgtaa gcagcggcgg ctggattctc
     4141 ggccttccac caaccgcctt gtgtttccct caattgcggg tcctttccgt agatcccccc
     4201 ggtttcctgg actggtaacc gcggttttga agccgccggc ctgtcttcgc aataccacca
     4261 attgtcggca aagcggaacg tctcgggggc cgtggccgaa cccacattga cgaaatcacg
     4321 caagtccgag cgg
//
LOCUS       DAOSUT010000248         4330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8651_length_4330_cov_8.740351, whole genome shotgun sequence.
ACCESSION   DAOSUT010000248 DAOSUT010000000
VERSION     DAOSUT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_4330_cov_8.740351"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..>1524
                     /locus_tag="PLD05_15095"
     CDS             133..>1524
                     /locus_tag="PLD05_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOP78812.1"
                     /translation="MKQRRDVFPIAVLGCLLVWGFSGLSALALEQDLLNLQPGQAVAV
                     DETFDGKSFTYEVVDVQTKEHCLVVRLRYPSPVVSELPQNNVIPVEYYLPKGIQANGP
                     RRPAVICLHILDGSLELVRMLASVLASHGVPAVVFQLPYYGPRGDSDGPYRILADPQH
                     FVSVLEQAFLEVKRAVDFLASRPEVNPQHIGVAGISLGAIIAASAAEREPRIYKAGLI
                     LAGGNLRQIVETAREAEPLRRFIADLDADQQASIWRILDTVDPLSQADLLKERAQQGR
                     VLMINAAEDEVIPRACTEQLAAALGIADRVFWLNDLGHYTALAALPRILELTAEFFAA
                     DLPADVRVKETPSAEMTPVQLIAATLQRLTEFYLREPTEGACHIGQIRAHVNGDEYTF
                     LFIRGAGESFRLEGRASDFGQVMLGQRDYPWMMARTGLVFVGQPDAGGKPQSPLSFVN
                     NALLERARFAVLAV"
     assembly_gap    1525..1624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1837..3075
                     /locus_tag="PLD05_15100"
     CDS             1837..3075
                     /locus_tag="PLD05_15100"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOP78813.1"
                     /translation="MLVVLRDRRFLLIFAILGWFALVSASLGRCGESSATELSGTRGD
                     RVEPRQAYQLLRVTPDGHGLLARHEGKTILVVSGTPEEMGKAHGSLLAPQIRYLVDRV
                     LYGVGAVDSVQTGVWFFEQMDEIHRRTQPFIPERYFVEMDALAQAAGLSQRDARYANL
                     FPERFHCSGVAVRGKASQNGRVIHARVLDYMRDINLQGCATIQVFIPEGRNAWISAGY
                     SGLIGTVTAMNEKGLAVGEMGGRGEGDWDGMPMTLLLREIMERAGTVQEAIEILKATP
                     RTCEYYYVLSDKTGELAAIRAVPQEVLVLGPGQQHPLLPHVPEDTVFISADERAKMLS
                     QRLQLFYGRIDVATMIEIIKRPVAMSSNLHNAIMLPELLEMWVADAGQTTPACDEPYV
                     RVNLREILDFYRHVVSSRGG"
     gene            complement(3083..4066)
                     /locus_tag="PLD05_15105"
     CDS             complement(3083..4066)
                     /locus_tag="PLD05_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78814.1"
                     /translation="MKKLAIVMLSGGLDSSLAARIIQLQGFDVIGLNIRLPFYNNVRS
                     AIRAARDLGIPLALREVGEDYLQVLRYPKHGYGRGANPCLDCRAYMLRMAADLMQELG
                     AMFIATGEVVGQRPMSQKRRDLGIVAHEAGLQGLILRPLCAKLLPPTIPEQEGWIDRE
                     KLYDFSGRSRKEIKALAKQLGLRHTPDAAGGCKLTEANFAPKVFDLLDHRDDATLWDC
                     ELLTVGRHFRLDERTKVVIGRNEEQNATLSRLYQRCPRQDCVFMIPGNFLGPHAIVVG
                     PADHSTLTKVAQLVIRYVHRPLPDPVEFEVRSGDGTCRLSWEKQSLVAVTT"
BASE COUNT          909 a         1090 c         1240 g          991 t
ORIGIN      
        1 cgattcgagc caccctcacc cccggcccct ctcccgccga acgggcgagg ggaggttgtg
       61 ggatgagctc ttaatcggga cgaggtgcgg cctagcggat tgagcgaatg gcggtttgag
      121 gtgcatgaca caatgaaaca gcgacgggac gtctttccaa ttgcggtgct gggatgcctg
      181 ttggtgtggg ggttctcggg cctgtctgcg ctagcgcttg agcaggattt gctcaacctc
      241 cagccaggtc aagcagtggc ggtggatgag acattcgatg gcaagtcatt cacctacgag
      301 gtcgttgacg ttcagacgaa ggaacattgc ctggtcgttc gcctgcgata cccgtcaccg
      361 gttgtttctg agttacctca aaacaacgtc atcccggttg aatattatct tcctaagggt
      421 attcaggcga atggaccacg acgcccggcc gtgatttgcc tccacatatt ggacggaagc
      481 ctggaattgg tccggatgct ggcctcggtc ttggcatccc acggagtccc ggcggttgtg
      541 tttcagttgc cctattatgg accacgcggg gacagtgacg ggccgtatcg gattctcgcc
      601 gatcctcagc atttcgtaag cgtgttggag caggcatttc ttgaggtcaa gcgtgcggtg
      661 gattttctgg cttctcgccc cgaggttaat cctcagcaca tcggggttgc ggggatcagt
      721 cttggggcca ttatcgccgc atcggcggcg gagcgggaac cgcggattta caaggcgggt
      781 ttaatcttag cggggggaaa tttgcgtcaa atagtcgaaa ccgcgcgaga agccgaacct
      841 ctgaggcgtt tcatcgcaga tttggacgcc gatcagcagg catcgatttg gaggatcctc
      901 gatacggtgg acccgctttc gcaggcggac ttgctcaaag agcgggcaca acaggggcgg
      961 gtcctgatga ttaatgccgc agaggatgag gtgattcccc gcgcgtgtac ggagcaattg
     1021 gcggcggcat taggcattgc cgatcgcgtg ttttggttga acgatttagg acactacacg
     1081 gccttggcgg ctttaccccg aattttagag ctaacggcgg agttctttgc tgccgatctg
     1141 cctgcggatg tgcgggtcaa ggagacacca tccgcggaga tgacgccggt acagttaatc
     1201 gcggcaacac ttcagagatt gaccgagttc tatctccgag agcctaccga gggggcttgt
     1261 cacattgggc aaattagggc ccacgtaaat ggtgatgaat acacattcct attcatccgg
     1321 ggggcgggag agagctttcg gctggaaggt cgcgcaagtg atttcgggca ggttatgttg
     1381 ggacagcgcg attatccttg gatgatggct aggaccggct tggtcttcgt tggacaaccc
     1441 gatgccggtg gtaagcccca atcgcccttg agctttgtaa acaacgcgtt gttagaacgg
     1501 gcaagatttg cagtcctggc tgtgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnncgcctt tttgatcctc cctctgacaa gccgcaaaag gtcgtttcac ggtacgatct
     1681 ttaccgcatg ttcggggcgg tcatcaattt tttggcagag aaagcccctt aaaggacatc
     1741 ggataggtaa cgataacatg ttgatgcgat aaggatttat gctcgagaac caattggcta
     1801 gttctttttg ggcccagacc aaggagaccg aaaaccgtgc tggtcgtttt acgcgatcgt
     1861 agatttttgc tcatttttgc cattttgggt tggtttgccc ttgtgtcggc ctcattgggt
     1921 cgatgtgggg aaagctcggc aacagagtta agcggtactc gtggggaccg ggtagagcct
     1981 aggcaagcct atcaactcct ccgcgtgacg cccgatggcc acggcctttt ggcgagacat
     2041 gagggcaaga caatcctggt cgtgagcgga acaccggaag aaatggggaa ggcccacggc
     2101 tcccttctgg cgccgcagat ccgatatttg gtcgaccgcg ttttgtacgg ggtgggagca
     2161 gttgactcgg tccaaacggg cgtttggttc tttgagcaga tggacgaaat ccatcgaaga
     2221 acgcagccgt ttataccgga gcggtatttt gtggagatgg atgccctggc tcaagccgcg
     2281 ggcttgtccc aacgcgacgc gcgatacgcc aatcttttcc ctgagcggtt ccactgtagt
     2341 ggcgtagcgg ttcgcggtaa ggcaagccag aatggtcgcg tcattcatgc ccgcgttcta
     2401 gattacatgc gcgacatcaa tctccaagga tgcgccacca ttcaagtatt tattcccgag
     2461 ggacgtaacg cctggataag tgccgggtat tcgggcttaa tcggcacagt cacggccatg
     2521 aatgagaaag gtcttgccgt cggcgagatg ggaggccgtg gcgaaggtga ttgggacggc
     2581 atgccgatga cgctgctcct tcgtgaaatc atggaacgcg ccggaacggt tcaggaggcc
     2641 attgaaattt tgaaggccac gccgcggaca tgtgaatact actatgtcct aagtgacaag
     2701 actggtgaac tggcagcgat aagggcggtg ccgcaagagg tcctggttct gggacccgga
     2761 caacaacatc ctttattgcc gcatgtgccc gaagacactg tcttcatttc ggctgacgag
     2821 cgagcgaaga tgcttagcca acgccttcag cttttttatg gcagaatcga cgtggctacc
     2881 atgatcgaaa tcattaaacg accggtggcc atgtcgtcga acctccacaa tgccatcatg
     2941 ttgccggaac ttctcgaaat gtgggtggcc gatgcgggac aaacaacacc cgcttgcgac
     3001 gaaccctacg tgcgggtcaa ccttcgggag attctcgact tctatcggca cgtggtttct
     3061 tcccggggcg gttaataaga gctcaggttg tcacggccac aagtgattgc ttttcccagg
     3121 acagacggca tgtcccatcc ccgctacgga cttcaaactc cacggggtcg ggcaatgggc
     3181 gatggacata ccggatgaca agttgggcaa cttttgtgag tgttgagtgg tcggcgggac
     3241 cgaccacgat ggcgtgcggg ccgagaaaat tgccgggaat catgaaaacg caatcttgtc
     3301 gggggcatcg ttggtatagt cgcgataagg ttgcgttttg ttcctcattc cggccgatca
     3361 cgactttcgt ccgctcgtcg agtcgaaaat gtcgccctac ggtcaagagc tcacaatccc
     3421 aaagcgtggc atcgtcacga tgatcaagca aatcaaagac cttcggtgcg aaattcgcct
     3481 cggtgagttt gcaccccccc gctgcatccg gagtatgtcg aagtcctaac tgcttcgcca
     3541 aggccttaat ttccttgcga ctccgcccac tgaagtcgta gagcttttca cgatcgatcc
     3601 agccttcttg ttccgggatt gtcggcggta aaagcttggc gcacagcggt cggagaatca
     3661 atccctgaag acctgcctca tgagcgacga tgcccaagtc acgtcgtttc tggctcatgg
     3721 gtcgctgtcc cacaacttcc ccggtcgcga tgaacatggc gcccaattcc tgcatcaaat
     3781 cggcggccat ccgcagcata tacgcgcggc aatcgagaca ggggttggcc cctcgcccgt
     3841 agccatgttt aggatagcga agcacttgga ggtagtcttc tccgacctct ctaagggcaa
     3901 gcggaattcc caagtcgcga gctgcacgga ttgccgagcg gacgttatta taaaaaggca
     3961 acctaatatt taggccaatc acatcgaacc cctgcaattg gataattcga gcggctaacg
     4021 aactatccaa tcctcctgac aacatgacga tagccagctt tttcatgttt gtgtgactca
     4081 agccagtttg ggccgtgact tccatttatc aaaaatagct ttgtcgcccg ggtaatttga
     4141 gggacttttt ccgtacggtg ctagatatcg cttgacattt ggcgttacac ccggcttggt
     4201 atgaactagt tcgtcacccg aatgatacgc cacaacagcg cgtagctctc catcagccaa
     4261 tgattcaagg gacgcgggtt gtcgcgttcg aggcgggctc tttataaggc caatcatttc
     4321 cgtaagatgg
//
LOCUS       DAOSUT010000249         4307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8694_length_4307_cov_9.476011, whole genome shotgun sequence.
ACCESSION   DAOSUT010000249 DAOSUT010000000
VERSION     DAOSUT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8694_length_4307_cov_9.476011"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..1800)
                     /locus_tag="PLD05_15110"
     CDS             complement(376..1800)
                     /locus_tag="PLD05_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOP78815.1"
                     /translation="MSLPCYASHEKVLIVPTVVTTLLLACTIASYAETTSGPENLVTN
                     PTFAGWKEGAAPPGWELFEPLIPETACTARPGEDGVVIEAPDRPFAVGGLRQKVTGIQ
                     AGKSYAVEVTGQIHNAAHPYQMILVRVTFTRGQQATHPAGWLIGGPDLTALHGSGKNW
                     SFRFSDIVTADEEADGAEITLAVKWPQGAKVIWQRVVMRPTDPLPPRKVKVGTVYLRP
                     RNSTPEKNLELFCAKLDEAGKLGLDIVCLGEAITLVGTNKSAADVLEPIPGPSTQRLG
                     EAARRNHLWVVAGLYERDGQTPYNTAVLINREGQLVGKYRKVHLPREEWIKGFTPGFD
                     YPVFETDFGKVAIQICYDWFFPEVAMIWRLKGAEIVFAPTWGNTLPDQDGRAEGETVF
                     RVRARDAGIYLVPSVYDGNSMIIDPMGRILASNQGKEALVWAEIDLNRRERLPWVGWW
                     RSIVPRHRVIPSYGPLLDPGPVPR"
     gene            complement(1920..2855)
                     /locus_tag="PLD05_15115"
     CDS             complement(1920..2855)
                     /locus_tag="PLD05_15115"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOP78816.1"
                     /translation="MRCRQCVFALGFVILVVAGAAGLEPGLRLNYPPDMPGAEVQTYK
                     KVDGVELKLYIYKPKDWKPSDRRGCIVFFFGGGWQSGSPAQFRPQCEYLASRGMVAIS
                     ADYRVASRHNAKVVDCVADAKSAIRWVRKNAEQLGVDPNKIAAGGGSAGGHLAACTAV
                     LPSGDDPSEDQSISSQANAAVLFNPALILTDLGLKDRVAGADAISRLERLQERIGGDP
                     KSVSPYHHIRPGLPPMIIFHGTADTTVPFLTVKLFAEAMTRAGNQCRLVPFEGAGHGF
                     FNYGRKDNTAYKETVRQMDQFLTSLGYLEGEPTIQ"
     gene            complement(2933..4012)
                     /locus_tag="PLD05_15120"
     CDS             complement(2933..4012)
                     /locus_tag="PLD05_15120"
                     /inference="COORDINATES: protein motif:HMM:NF015617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HOP78817.1"
                     /translation="MSAVDGVRVRSGVAVTLLVLAFAVFPVPIWAGEDIDGERRASPY
                     ETGSFRWTVGPPVLAARSSTPEMTYYSIKDPSVVRFDDRWHIFCTVRGRPRTHQVEYL
                     SLSDWQDPKPRREMLEIIPGYYCAPQVFYFRPHKKWYLLYQTSDPQRKPALQPAISTN
                     DRVDGVGKWSQPEFLFATGPNNVERWIDFWIICDDQRAYLFFTSLDGRFWRCETSLRD
                     FPHGWNEPKVVLQTDIFEAAHVYSLKGRSQYLAIIEAQNGSRRYYKAFLADRLDDDWR
                     PLAAERNKPFLGPQNVRFLGEEWCQSFSHGELLRDGYDERMEVNPDRLVMLFQGVSDR
                     EMAGKPYGEIPWKLGLAYQEVPGEP"
     gene            complement(4110..>4307)
                     /locus_tag="PLD05_15125"
     CDS             complement(4110..>4307)
                     /locus_tag="PLD05_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HOP78818.1"
                     /translation="YQQSVSLRDALKKVGVPVALITVEKGGHGGFRNPEIERRVDSFF
                     DAHLRDIKAEFKDETLPNESK"
BASE COUNT          936 a         1239 c         1220 g          912 t
ORIGIN      
        1 caaaatcacg tgtattatac atcggcggat aaagtccacc acggagacgg gcttacggcc
       61 tatcctataa tctcccctcg cccgttcggc gggagagggg gactttttga gatagattct
      121 tgggtgatct ctgtaaatcc gaagacgcca agtcattcag tcggccacga cgcggcgtat
      181 atgaatggcg ccgccgcgtg gtcctttgta accaggttat gcatcggtag cgccactgcc
      241 cgcggttcaa cttgctttat ttcaccggcg ggcgagcatg ggggcgcccc caagaaatag
      301 gcggaaaagg tacagaggcc gaaaacgcct cctcagctta cagggagggc tcgcgactac
      361 gatggaggct caggatcagc ggggcaccgg accagggtcc aaaagagggc cgtaactggg
      421 aatcacccga tgacgcggaa cgatgctccg ccaccagcca acccagggca agcgctcccg
      481 ccggttgaga tcgatttccg cccaaacgag ggcctctttt ccctgattcg acgccagaat
      541 gcggcccatc ggatcaatga tcatactgtt gccatcatac accgagggga cgaggtaaat
      601 cccggcgtcg cgggctcgga cgcggaacac ggtttcacct tccgctcgac catcctgatc
      661 gggcagcgtg tttccccacg tgggggcgaa gacgatctcg gcgcctttaa gccgccagat
      721 catggccact tcggggaaga accaatcgta gcagatttgg atcgccacct tgccgaagtc
      781 tgtttcaaag acggggtagt cgaaaccggg cgtaaaaccc ttgatccatt cctcgcgggg
      841 tagatggacc ttgcggtact tgcccaccaa ttgaccctcg cggttgataa ggacggccgt
      901 attgtaaggg gtttgcccat cgcgttcgta aagtcccgcg acaacccaaa ggtgattgcg
      961 gcgggcagcc tcgcccagcc gttgcgtgga agggccgggg atcggttcga ggacgtctgc
     1021 cgccgactta tttgtgccga caagcgtgat ggcttccccc aggcagacaa tgtccagtcc
     1081 cagtttaccg gcttcatcca gcttagcaca gaaaagctcc aggttttttt cgggtgtgct
     1141 gtttctcggt ctaagataga cggtacctac tttgactttc cgcggtggga ggggatctgt
     1201 aggacgcatg acaacacgct gccaaatgac tttggcccct tggggccatt tgacggcgag
     1261 ggtgatttcg gccccgtcgg cctcttcgtc ggctgtcacg atgtcggaga agcggaacga
     1321 ccagtttttg ccggatccat gaagggcggt gaggtctggt ccaccgatca accagcctgc
     1381 cggatgggtc gcctgctggc cccgtgtgaa cgtgacacgc accaagatca tctggtacgg
     1441 atgtgccgca ttgtggattt gtcctgtcac ctcgaccgcg tagctcttac cggcctggat
     1501 gcccgtcact ttttgtcgca gtccgcccac ggcaaacggc cgatcggggg cctcaatcac
     1561 gacaccatcc tctccgggac gagcggtaca cgccgtttct gggataaggg gctcaaacag
     1621 ctcccaaccc ggaggagccg caccttcctt ccagcctgcg aacgtgggat ttgtcacaag
     1681 attttccggg ccagaggtgg tttcggcata agatgcaatc gtacacgcca gcaacaaagt
     1741 tgtgaccacc gttggcacga tcaaaacttt ttcatgcgaa gcatagcacg gcagcgacat
     1801 ggctcgttct cctccgatct aacgacctat cggcaagggg tggccaatag accaacgcgc
     1861 gtctcggccg gacgaccctt cacaaagagt gcagtcgatt ctcatatcgt gttgtgaatc
     1921 tactggatgg tgggttcacc ctcaagataa cccagacttg tgaggaactg atccatctgg
     1981 cgaacggtct ctttataggc cgtgttgtcc tttctgccat agttgaagaa gccgtgtccg
     2041 gccccttcga acgggaccaa acggcattgg ttgcctgccc gggtcatggc ttcggcaaag
     2101 agcttgacgg tcaggaaagg cacggtggta tcagcggtgc catggaagat gatcatcggg
     2161 ggcaagcccg ggcgaatgtg gtggtaaggc gaaacgctct tggggtcgcc gccaatccgt
     2221 tcctgaagcc tttccaaccg acttatggcg tcagccccgg caacgcggtc cttgaggcca
     2281 agatcggtga gaataagggc cggattgaaa agcacggcgg cattggcctg tgaactgatg
     2341 ctttgatctt ccgagggatc atcaccggag gggagaacgg cagtgcaggc ggccaggtgt
     2401 cccccagccg agccgccacc cgcagcgatt ttgttgggat cgacgccgag ttgttccgcg
     2461 ttctttctga cccaacggat ggccgatttg gcgtcggcca cgcaatcaac gaccttcgca
     2521 ttgtgacggc tcgcaacgcg atagtccgcc gagatggcca ccatgccacg cgaggccaga
     2581 tactcacatt ggggtcggaa ctgagccggc gacccgcttt gccagcctcc accaaagaag
     2641 aagacgatgc acccacggcg gtcgctcggc ttccagtcct tgggtttgta gatgtaaagt
     2701 ttcaactcca ccccatcaac tttcttgtat gtctggacct ccgccccggg catgtccggc
     2761 gggtagttca ggcgaagacc aggttccaac cctgccgcac cagcaacaac taggatgaca
     2821 aatcccaagg cgaaaacaca ttgacggcaa cgcatgactg aatcctccca ctgtatcttt
     2881 gacggacacg aggaattaac cactccttaa actcccaaag gcgtagttcc gactatggtt
     2941 ccccgggcac ctcctgataa gccaaaccca gtttccatgg gatttcaccg taaggttttc
     3001 ctgccatttc tcggtcactg accccttgaa agagcatcac caaccgatcc gggttgacct
     3061 ccatccgttc gtcgtagcca tctcgcagta gctcgccgtg gctgaacgat tggcaccact
     3121 cctcgccaag gaacctaaca ttctggggtc cgaggaaagg tttgttccgt tctgcggcca
     3181 gaggacgcca gtcgtcatcc aatcggtcag ccaggaaggc cttataatag cggcggcttc
     3241 cgttttgggc ctcgatgata gcgagatatt gggatcggcc cttcagggaa tacacatgcg
     3301 ctgcttcgaa gatgtcggtc tgaagaacga ccttgggttc attccatccg tggggaaagt
     3361 cacgtaagct ggtttcgcat cgccagaagc ggccatcaag cgaggtgaaa aagaggtaag
     3421 ctcgttggtc gtcgcatatg atccaaaaat cgatccagcg ttcgacattg ttcgggcctg
     3481 tggcgaagag aaactcaggt tgcgaccact tgcccacccc gtccacacgg tcattggtgg
     3541 aaatcgccgg ttgaagagca ggcttacgct gcgggtcgct tgtctgatag agcaagtacc
     3601 attttttgtg aggccgaaaa taaaaaacct gcggcgcaca atagtaaccg gggatgattt
     3661 ccaacatctc gcgccgtggt ttggggtctt gccagtcgct gagactgaga tactccactt
     3721 gatgcgttct gggacgacca cggaccgtgc agaagatgtg ccatcgatcg tcaaagcgga
     3781 ccacactggg gtccttgatc gagtagtagg tcatttccgg tgtgctgctc cgtgccgcca
     3841 ggaccggtgg ccccaccgtc cagcgaaagg aacctgtctc gtaggggctt gcgcgccttt
     3901 cgccatctat gtcctcgcct gcccagatag ggaccgggaa tacggcaaag gccaatacca
     3961 gtagcgtcac cgccacacct gagcggacac ggacaccgtc aaccgcgctc atcagtttgt
     4021 tcctccctag acaatgaaca tgcataatct tggcgagatg acttatcgtg ttgcacgatg
     4081 agtcacacaa acgggtgctg aactgatgat cacttacttt cattgggaag cgtttcatcc
     4141 ttgaattcgg ccttgatgtc gcgtaagtgt gcgtcgaaga aggaatcgac ccgccgctcg
     4201 atttctgggt tacgaaaacc accatggcca cccttctcga cggtgatcaa ggccacaggg
     4261 acgccgacct tcttcagcgc atcgcgcaga gataccgact gctgata
//
LOCUS       DAOSUT010000250         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_8877_length_4226_cov_10.105251, whole genome shotgun sequence.
ACCESSION   DAOSUT010000250 DAOSUT010000000
VERSION     DAOSUT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8877_length_4226_cov_10.105251"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="PLD05_15130"
     tRNA            complement(1..75)
                     /locus_tag="PLD05_15130"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Glu,seq:ttc)
     gene            246..3509
                     /gene="mfd"
                     /locus_tag="PLD05_15135"
     CDS             246..3509
                     /gene="mfd"
                     /locus_tag="PLD05_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOP78819.1"
                     /translation="MQTVESGVDGGLESLHELTRKLQTHPEFQRIIDAFHSGKGGQID
                     GVVGAGSALFAAAVADSTQHCVLVVLPNAQAALGFCEDLKLFTTQTATYFPRLHRLQV
                     PLNPDDEALGDRLRILRSLISSQAPTLIVTSIEAILQAVPLVDELRSRVVSLRVGDTL
                     NVSDLLQRLSDCGFRSVATVALPGEFSLRGGILDIFSAEWSAPLRIELFGDQIESLRR
                     VDLETQRSVERLNEVQFVLPKRETELTATLFQYLPKDAWLILADPQAIDDTARDYFRK
                     LDTDQGHVPIEQIWTQCGQFSSMELWDIAPDSGPTVFHLGMQTVERFSGQIERIQAEL
                     DSIGQDENVRIVCPTTAEVARLSELLRETRTAKEGRLRFVQGRLTSGFRVPWDHLVIV
                     GAHELFERVSRDIAPRRFVSRPINDFLDLREGDLVVHVAHGIARYRGLVLLEKGEQAE
                     EHLLLEFAEGTKLYVPASKIGLVQKYVGGTRARPKLSKLGGQAWQRQKKAVAEAVFDL
                     AAEMLELQAIRETRPGIAFPPDSIWQQEFDASFPYEETPDQLEAMEAIKADMMRPRPM
                     DRLLCGDVGFGKTELAMRAAFKAVDAGYQVAVLVPTTVLAEQHLRTFRERMAEFPIRI
                     EVLSRFCTAKQEAAVLEGLANGSIDIVIGTHRLAQPDVVFQNLGLVIIDEEQRFGVEI
                     KERLKSFRNTVDVLTMTATPIPRTLHMALVGLRDISNLATPPQDRLAVETHICVFDTQ
                     LIRQAVLRELNRGGQIYFVHNRVNDIDAVYRILCSVVPEARIRIAHAQLPDHELEEVM
                     LSFVRHECDLLLTTTIVESGLDIPNANTIFIDHAERFGLADLHQLRGRVGRYKHHAYC
                     YLLIHPHQLLNPNARRRLQAIAELSHLGAGFALAMKDLEIRGAGNILGTEQSGHIAAV
                     GYELYCQLLDHAVRRLRNQPPPTHIDVNIDLPCQACLPETFVSDHRTKIDIYRKLANL
                     SDPAQVDDFAEELRDRFGPLPPPVIRLLKLQEIRIHAHWWRIREIRQENNFVVFHFVS
                     RKKMEALRAMVDGHLRIVDAQSAYLVADDWVRDVDRLISELKSLLQRRPTNLYNSAS"
     gene            3673..>4226
                     /locus_tag="PLD05_15140"
     CDS             3673..>4226
                     /locus_tag="PLD05_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78820.1"
                     /translation="MAVCAEVVRKLLGPVAQRADASETSGKATQGDSLEAPNSSWYER
                     RTSPWREQRQLPPWPPRFDDATSQNTGVARAESSRNTDLAPPPMANQVVGDAHQLYPA
                     TARPEFVLPTGRLVTVIDPMQAEAVPLASQVTDDGANLPSVQARLSPSSNPPPVFPSV
                     EIWQVPSDDDQSRQVNLASQQEVVV"
BASE COUNT          893 a         1106 c         1232 g          995 t
ORIGIN      
        1 tgaccccgac gggatttgaa cccgtgttac aggcttgaaa ggcctgtgtc ctagtcctgg
       61 ctagacgacg gggccaaaga aaagttaatt atcgccgctg gacggacgtc agtcaaccgc
      121 gggtgcggtc gtgactttgg tcgcgcctga aagacaaaat taaaccggat acacaatgtg
      181 gtactgtggt gctcgaaggc tagctactct tggcggcagg acgcccaccg taatacaatt
      241 cgcgcatgca gaccgtggaa tccggtgtcg acggtgggtt agagtcactg catgagttaa
      301 cacgcaaact tcaaacccac ccggagtttc agcgaattat tgacgctttc cactccggta
      361 agggggggca gatcgacgga gtggtggggg cagggtctgc gctcttcgcc gctgccgttg
      421 cagattccac ccaacattgt gttctggtcg tgcttccaaa tgcccaggcg gccctgggct
      481 tttgtgagga tttgaaactt ttcaccactc agactgctac ttactttccc aggttacacc
      541 ggcttcaggt tcccctaaat cctgacgacg aggcccttgg tgatcggcta agaatcctgc
      601 ggtcactgat atcttcccag gcccctaccc tgatcgtcac ttcgattgag gccattctcc
      661 aagctgttcc actggtcgat gaactgcgca gtcgtgtggt gtcactcagg gttggcgata
      721 cgctgaatgt cagcgatctc cttcagcggt tgtccgactg cggtttccgc agtgtggcca
      781 ccgtggccct gcccggcgag ttttcgctcc gtggtgggat tctcgacatc ttttccgcag
      841 aatggtcggc acctttgcgg attgagttat tcggggatca aattgagtcg ctcaggcggg
      901 tagacctgga gacgcagcga agcgtggaac ggctgaacga ggttcagttc gttctaccta
      961 aacgggagac ggagctaacg gccacactgt tccagtactt gcccaaggat gcttggctca
     1021 ttctggcaga tcctcaggcc attgacgata cagcgaggga ctacttccgg aaactcgata
     1081 cggatcaggg ccatgtgccc attgagcaga tttggactca gtgcgggcag ttctcttcca
     1141 tggagctatg ggatatcgca ccggacagtg gcccgacagt ttttcacctg gggatgcaaa
     1201 cagtcgagcg gttctctggt caaattgaac ggattcaggc agaactcgat tccatcgggc
     1261 aagacgaaaa cgtgcggatt gtttgtccga cgacggccga ggtcgctcgg ctgagtgaat
     1321 tgttgcgaga gacgaggact gctaaagaag ggcgtttgcg gtttgttcag ggacggctta
     1381 cttcagggtt tcgcgtgccg tgggatcatc ttgtcattgt tggggcgcat gaactgtttg
     1441 agagggtgtc gcgcgacatc gccccccggc ggtttgtgag ccgtccgatc aacgatttct
     1501 tagatcttcg cgagggagat ttggtggtcc acgtagccca cggaattgca cgctaccgtg
     1561 ggctagttct cctggagaag ggagaacagg ccgaggagca tcttttgcta gagtttgcgg
     1621 aagggaccaa gttatatgtt ccggcttcga agattggcct agtgcagaag tacgtcggcg
     1681 gcacgagggc tcggccgaag ctttcgaagc taggggggca ggcatggcaa cgccagaaaa
     1741 aggcggtggc cgaggccgtt ttcgatctcg cagcagaaat gttggagtta caggcgattc
     1801 gggaaacgcg tcccggaatt gccttcccgc cggactccat ctggcaacaa gagtttgacg
     1861 cctcctttcc ctatgaggag acgcccgatc agttggaagc catggaggct atcaaggccg
     1921 acatgatgcg acctcgccct atggatcggc tcctctgtgg ggacgtcggg ttcggcaaaa
     1981 cggagctagc gatgcgggcg gctttcaagg ccgttgacgc aggctatcag gtggcagttc
     2041 tggtgccgac aacggtcctt gccgaacaac acctaagaac attccgcgag cggatggcag
     2101 agtttcctat tcgtattgaa gtcctgtccc gattctgcac agctaagcag gaggccgccg
     2161 ttctggaggg gcttgctaac ggttccatcg acattgtcat tggaacgcat cgcctcgctc
     2221 agccggatgt ggtatttcag aatctgggtc tggtgattat tgacgaggaa cagcgctttg
     2281 gggttgagat taaggagcgt ctcaagtctt tccgcaacac ggtggatgtg cttacgatga
     2341 cggccacgcc gatcccacgg acgcttcaca tggcccttgt gggactccgg gacatcagca
     2401 acctggcgac accgccgcaa gatcggttgg ccgtggagac tcacatttgc gtttttgata
     2461 cacaattgat ccgacaggcc gttctccgcg agttaaaccg cggcggtcag atttacttcg
     2521 tgcataatcg ggtcaacgac attgacgcgg tttatcgtat tctgtgctca gtggtacctg
     2581 aagcccgtat tcgaattgcc cacgcacaac tgccggatca tgagcttgaa gaggtgatgt
     2641 tgtcttttgt gcgacatgaa tgtgacttgt tactgacaac gaccattgtg gaaagtggtc
     2701 tcgatattcc aaacgccaat acgattttca tcgatcatgc cgagcgattt ggactggctg
     2761 acctacatca gttgcgcggt cgtgtggggc gatacaagca ccatgcatac tgttacctgc
     2821 tgatccatcc tcatcagctg ttgaacccaa acgcacgcag acgactgcaa gccattgccg
     2881 agctgagtca tctcggtgct ggttttgccc tcgctatgaa ggatttggaa atccgcggtg
     2941 ccgggaatat tttggggacg gaacagagtg ggcacatcgc ggcggttggg tacgaacttt
     3001 actgccagct tctggaccac gctgtgcggc ggctcaggaa ccagccgccc ccaacgcaca
     3061 ttgacgtgaa tatcgatctc ccctgtcagg cgtgccttcc tgagacgttc gtatccgatc
     3121 ataggaccaa gattgacatt taccgcaagt tagcaaatct ttcagatcct gcccaggttg
     3181 acgattttgc cgaggaatta cgagaccgat tcggccctct accaccgccg gtgattcggt
     3241 tgctgaagtt gcaggaaatc cgtatccacg cccattggtg gcgaattagg gagattcgtc
     3301 aggagaacaa tttcgtggtc tttcactttg tgtctcgcaa gaaaatggag gccttgcggg
     3361 ccatggtgga tggtcacctc cgtatcgtcg atgcccagtc cgcatatctt gtcgccgatg
     3421 attgggtccg tgacgtcgat cgccttattt ccgaactaaa atcgctgttg cagcgtcgtc
     3481 ccacgaacct atataattcc gcctcctaat tctgcaacaa ggcgaggaag tgttttttgt
     3541 gatattaagc agagactggg ttgagattga ggtgaatacg gttcggtgcg acaactcatc
     3601 tggccgatat tagcggttgt gcttgtggcg tgtgtaacgg tgccgtcctg ttcgaaaagg
     3661 acagaagacg cggtggctgt ctgcgcagaa gtcgtgcgca aattgctggg acctgtcgct
     3721 caacgagcgg acgcctcgga aacttcagga aaggccacac aaggcgattc tctagaggca
     3781 ccgaacagtt cctggtatga gcgtcggacg tctccttggc gtgaacagcg ccagcttccc
     3841 ccatggccgc cccgatttga tgacgcaacg tcgcagaata ctggggttgc ccgggcggaa
     3901 agctcacgca ataccgacct tgcacctccc cctatggcca atcaggtggt aggagatgcc
     3961 caccagcttt atcccgcaac agcccgacca gagttcgtct tgcccacagg gaggcttgtg
     4021 acggtgatcg atccgatgca ggcagaggct gtcccgctgg ccagtcaagt tacggatgac
     4081 ggggccaatt tgccctccgt gcaagcgaga ctatcgccta gcagcaatcc tcctcccgta
     4141 tttccttcgg ttgagatatg gcaggtccct tcggatgatg atcaatctcg gcaagtcaac
     4201 ttggcatctc agcaggaagt tgtggt
//
LOCUS       DAOSUT010000251         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9049_length_4143_cov_8.771037, whole genome shotgun sequence.
ACCESSION   DAOSUT010000251 DAOSUT010000000
VERSION     DAOSUT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9049_length_4143_cov_8.771037"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   32..202
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=35..70
                     /rpt_unit_seq="gctgtggtttgcagggaatcgtcgctcggatacact"
     gene            complement(253..594)
                     /gene="cas2"
                     /locus_tag="PLD05_15145"
     CDS             complement(253..594)
                     /gene="cas2"
                     /locus_tag="PLD05_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOP78821.1"
                     /translation="MARSEPISGYKIMWLFVFFDLPMNDRAARREYVRFRKRLLELGF
                     TQLQYSVYARPFPSEEALAPYRQEIREALPPDGEVRLLPVTDRQFGKMENYVGKLRTE
                     TEKPPRQLMLF"
     gene            complement(581..>1471)
                     /gene="cas1"
                     /locus_tag="PLD05_15150"
     CDS             complement(581..>1471)
                     /gene="cas1"
                     /locus_tag="PLD05_15150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOP78822.1"
                     /translation="DLATQPARLSVRHRQLVIEMGEQATTTVPLAEVATLIVCHSQIT
                     LTQSVLAGLAEAGAVCIVCDAKSRPVATMFPLVTHHLQTERLAAQARASVMTRRGIWR
                     QIIRNKIESQAHTLEELYGDDHGLRQLARRVKSGDASNAEAVAARRYWPRLFGDPSFR
                     RKPRGEAQNRYLNYGYAVLRAITARAITATGLHPSLGIHHHNRYNPFCLADDLMEPFR
                     PRVDLMVARLLKEYPPDAELTPEIKARLLSFAEERYDLDGERRTLFDVLHRVAASLAE
                     VFLKRTAHLLLPKWDKHGAE"
     assembly_gap    1472..1571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1572..>4143)
                     /gene="cas9"
                     /locus_tag="PLD05_15155"
     CDS             complement(<1572..>4143)
                     /gene="cas9"
                     /locus_tag="PLD05_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOP78823.1"
                     /translation="DLGVVSLGWAAIEVNGQGPKGLLAAGVRCWDTSDASPSNAEQGK
                     KKPANQTRRESRQTRRQLFRRAQRLRRTYRTLQDMGLVPRGPRSSTVRKATLDAIDQQ
                     AKEWLKQNVPALANDDLLDHTFVYRLRAAALDYALPPQLLGRVFFHLAQRRGYLSNRR
                     SAPKEEKHGEVEKGISELEQNIRASGARTLGEYFSRVNPLEQRIRARWTSRKMYQHEF
                     ELIWNAQRPHHPTVLTDENKEKLFKALFFQRPLKSQKGLVGRCTLECLTKDLGNGNVE
                     RIYPFRRAPMACLEAQRIRYMQRINDLTLIDPKGDMRSLTQEERQTLYALAETHEKLT
                     FDAIKKVLGLTGEGWKFSMERGGEKELPGNKTAVRIRRIIGTKTWENLSESQRKELVD
                     TIIAYLSPEALAARLQKVWHFAPEVADKLSKVTLESGYHKYSRRAIRKLLPLLEKGDS
                     LNTAIQKIYGGTQRKTDVHDLLPPVQEVVKDVRNPLVVRALTELRKVVNALIRKYGKP
                     QLIRIELARDLKNPRKVREKRAEEMRQREKQRKAAAAKIQEVLGREAKGADIEKYLLA
                     EECNWECPYTGRPITLEALLGDQPQFDVEHIWPFSRSLDNSFANKTLCYHQENRDVKK
                     NRTPFEAYGSNQQRWHEILARVERFKEPFKSEKLRRFKAESIPEDYPQRDLNDTRWIS
                     REAADYLGHLYGGRIDPSGKQRIFAIKGQLTAIVRQAWDLNSILGTGPEKERNDHRHH
                     AIDAITIALADASLVQRISWAAARLPKTFSRITVQIDPPWEGFLNDVRQQIHNIVVSY
                     RPNRRVSGPLHDANFYTPPIGGQKPKHRKQLKDLSPKEVSKIVDDRIRTLVQETLERH
                     G"
BASE COUNT          810 a         1081 c         1167 g          985 t
ORIGIN      
        1 cacttcctct ggtagaagtc ccgttttacg tgaagctgtg gtttgcaggg aatcgtcgct
       61 cggatacact aagctggaat cgaggcctta aaggtctttt gctgtggttt gcagggaatc
      121 gtcgctcgga tacactgcgg gaacgaaaag gggagcgatc atcgacgctg tggtttgcag
      181 ggaatcgtcg ctcggataca ctatttgcga cgtaacttcc taacaagacg ataacttacg
      241 acaaattcgc gctcaaaaaa gcatgagctg acggggcggt ttttcggtct ctgtgcgtaa
      301 ttttcccaca taattttcca tcttcccgaa ttgccgatcg gtgaccggca gcagtcgaac
      361 ctcgccgtcc gggggcaggg cttcccggat ttcttgccga tatggtgcca gcgcctcctc
      421 gctggggaaa ggtcttgcgt aaaccgagta ctgcaactgc gtaaagccca actccagcag
      481 tcgtttgcgg aaccgcacgt attcgcggcg ggccgcacgg tcgttcatcg gtaggtcgaa
      541 aaagacaaat agccacatga tcttgtaacc ggaaataggt tcactccgcg ccatgcttgt
      601 cccactttgg taaaaggaga tgtgccgttc gtttgagaaa aacctcggcc agcgatgctg
      661 caacccggtg caatacgtca aaaagcgtcc gtctttcacc gtccaggtca tagcgctcct
      721 cggcaaagct gagcaatcgc gccttgattt ccggggtcaa ttccgcatcg ggtgggtact
      781 ccttaaggag ccgggcgacc atgaggtcca cgcgggggcg gaatggttcc ataaggtcgt
      841 cggccaggca aaatgggttg tagcgattgt ggtggtgaat gcccaagctg gggtgcaggc
      901 cggtggccgt aatggcccgc gctgtgatcg ccctgagaac ggcataaccg tagttgagat
      961 agcgattttg agcctctcca cgcggtttgc gtcgaaatga gggatcacca aagagccgtg
     1021 gccagtagcg ccgcgcggcc acagcctcgg catttgaggc atcgcccgat ttcacacgcc
     1081 gggccaattg tcgcaggcca tgatcgtccc cataaagctc ctccaaggtg tgtgcctgag
     1141 attcgatctt attgcgtatg atttgccgcc agatccctct tcgtgtcatg actgaggcgc
     1201 gggcctgtgc ggccaaacgt tccgtctgaa ggtggtgagt gacaagcggg aacatggtcg
     1261 ccaccgggcg acttttggcg tcacagacaa tgcatacggc accggcctcg gccagaccgg
     1321 cgaggacaga ttgcgtgagc gttatttgcg aatggcatac aatgagggtc gccacctcgg
     1381 caagcggcac ggtggtggtc gcctgctcac ccatttcaat gacaagttgt cgatgccgca
     1441 cagacagccg ggccggctgt gttgccaaat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nccatgacgt tcgagggtct cttgcacaag cgttctaatg cggtcatcga
     1621 caattttcga gacttctttg ggcgataaat ctttcaattg tttgcgatgc ttgggttttt
     1681 ggccaccaat aggtggggtg taaaagtttg cgtcatgcaa aggacccgag acccgacggt
     1741 tagggcgata agagacgacg atattgtgaa tctgttgccg gacgtcgttc agaaagccct
     1801 cccacggcgg atcgatctgc acggtgatgc gtgaaaacgt tttgggaagt cgggcggctg
     1861 cccaagatat cctctggacc agcgaggcgt cggccagcgc gatggtgatg gcatcgatgg
     1921 catggtggcg atgatcgttg cgttctttct cgggccccgt gccgagaatg ctattgaggt
     1981 cccaggcctg acgcacaatc gccgtgagtt ggcctttaat ggcgaagatt cgctgctttc
     2041 ctgaagggtc aatgcgacct ccataaagat ggccgagata atcggcggcc tctcgggaaa
     2101 tccagcgggt atcgttgagg tcccgttgcg gatagtcctc tgggatgctt tcggccttaa
     2161 agcgtctcag tttttctgac ttaaagggct ccttgaacct ttccacccgc gcgaggatct
     2221 catgccagcg ctgctgattg cttccatacg cctcgaaagg cgtgcggttc tttttgacgt
     2281 ctctgttttc ctgatggtag cagagcgtct tattggcaaa ggaattatcg agagagcggc
     2341 taaacggcca gatatgctcc acatcgaatt ggggctgatc gccaaggagg gcttctagcg
     2401 tgatcgggcg tccggtatag gggcactccc aattgcactc ttcggccagg agatattttt
     2461 cgatatctgc tcccttggcc tctcgaccca agacctcctg aatcttggcg gcggcggctt
     2521 ttctttgttt ttcccgctga cgcatttctt cggcacgttt ttcgcggacc ttgcgcggat
     2581 tcttgagatc gcgggccaat tcgatccgga tgagctgcgg cttaccatat ttgcggatca
     2641 gggcattgac gaccttgcgc aactctgtca gggctcgcac aaccaacggg tttcggacat
     2701 ccttcacgac ctcttgaaca ggcggcaaaa gatcatggac gtccgttttc cgctgcgtgc
     2761 ccccgtagat cttttgaatt gccgtattca gggagtcgcc tttctccaaa agtggcaaga
     2821 gcttgcggat agcgcggcgg ctgtacttgt gatagccgct ctccaaagtg accttgctca
     2881 gtttatcggc cacctctgga gcaaaatgcc aaaccttttg aagacgcgct gcaagtgcct
     2941 cgggactaag ataggcgatg atggtatcga ccagttcttt tcgttgtgac tcgctgaggt
     3001 tttcccaggt tttggttcct atgattcttc gtatccgcac ggcggtttta ttgccgggca
     3061 gttccttttc gccgccccgc tccatggaaa acttccagcc ttcacccgtc aacccaagca
     3121 ccttttttat ggcgtcgaag gtcagttttt cgtgtgtctc ggccagggcg tacaaggttt
     3181 gacgttcttc ctgggtcagg cttcgcatgt cgcctttagg atcaatcaag gtcagatcgt
     3241 tgatcctttg catatagcga atgcgttgcg cttcaaggca agccatcggc gccctgcgga
     3301 aaggataaat cctttcgacg ttgccattgc cgagatcttt cgtcaggcat tccagcgtgc
     3361 atcgaccaac aaggcctttt tgggacttta gaggccgttg gaagaacaag gccttaaaga
     3421 gcttttcctt gttttcatct gtcaaaaccg tgggatgatg ggggcgctgc gcattccaga
     3481 tcaattcgaa ttcatgctga tacattttgc gtgacgtcca gcgtgctcgg atacgctgct
     3541 ccagcgggtt gacgcgggaa aagtactcac cgagggtgcg ggcaccgctc gcccgaatgt
     3601 tctgctcaag ttctgagata cccttttcga cctccccatg cttttcttct ttgggtgcgg
     3661 aacggcgatt gctaagatag ccgcgacgct gggcaaggtg gaaaaacacc cggccgagca
     3721 attgtggcgg taatgcgtaa tccagggctg ctgcccgcaa acgatacaca aaggtatggt
     3781 ccaacaagtc gtcgttggcc aaggcgggga cgttttgctt taaccactcc ttggcctgct
     3841 gatcgatagc gtcgagtgtt gccttgcgca cggtgcttga gcgcggaccg cgcggtacaa
     3901 ggcccatatc ttgtagggta cggtaggtgc gacggagccg ctgggccctg cgaaagagct
     3961 ggcggcgggt ttgccgcgat tcccgccggg tctggttggc gggtttcttt ttgccctgct
     4021 cagcgttgct cggtgaagca tcactagtgt cccagcaacg gacgcctgcg gcaagcagac
     4081 ccttgggacc ctgcccattg acttcgatgg ctgcccagcc cagtgagacg acccccaaat
     4141 caa
//
LOCUS       DAOSUT010000252         4046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9295_length_4046_cov_8.970684, whole genome shotgun sequence.
ACCESSION   DAOSUT010000252 DAOSUT010000000
VERSION     DAOSUT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9295_length_4046_cov_8.970684"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1901)
                     /gene="cls"
                     /locus_tag="PLD05_15160"
     CDS             complement(375..1901)
                     /gene="cls"
                     /locus_tag="PLD05_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOP78824.1"
                     /translation="MAIGEWTTYWTISEWIIRLAMLPVIVMRKERPATCLAWLTVVFF
                     APLPGLLLYLLVGENRLGRRRLEIRKSRWAKLESVNAPPVGAHYQSDSSELWGEPARE
                     DADEADVADDSLQTGTMGKGGLDPELQPLSDLAFRLTGWPVTTENQLSFLCDPLLTID
                     RLVAEIDSANDHVHLLYFIFRDDEIGRRVADALVRAAQRGVQCRVLVDAVGSRPMLKR
                     LAPRLRGAGVEVHPALPVRLYRIPFARLDLRNHRKLAIIDGRVAYTGSQNIVEPTYGR
                     RKAGPWHDITARIVGPSVPHLQLLFLEDWFHETGQWLDEPRYFPVISEGGQTILQVLP
                     SGPDMPTELFQDLLVKAIFLARRRVILTSPYFVPDEPLTMALRLAAYRGVQVDLVLPL
                     RSDHPLVDAAGEFHWRRLSQHGVRVHFFQEGLLHAKTLTIDNRLGLFGSANYDIRSFH
                     LNFEVNLLIYSTEAVQALKELQLRYLAASRPLDSAREKEPRPVRKLTIHLAKLLSPLL
                     "
     gene            complement(2257..3294)
                     /locus_tag="PLD05_15165"
     CDS             complement(2257..3294)
                     /locus_tag="PLD05_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOP78825.1"
                     /translation="MKAAFFTGLRQVGILETPPAQIERPQDVLVHVERVGVCGSDVHY
                     FLHGRIGDQILQYPASVGHECAGTVVDVGPKVTRVKVGDLVAIEPALVCNECDQCRAG
                     RSNTCRRLQFLGCPGEAPGAAREYYALPESNCFPLPAGLTLDHGALAEPLSIGLYAVR
                     LAQLQSGDRVAILGCGPIGLSVLTWVKLTAGCRAYVTDLLDERLEAAARCGADWTGNA
                     AREDVATSIERREPLGVDCVFECSGDAGCVTQAIRLAKPGGRVLWVGIPPEEHVSLPA
                     HLARRKEITLQLVRRQRDCLKPVLHAMAQGQINPTWWITHRYPLNRIQEAFELVAEYR
                     DGVIKAMIELP"
     gene            complement(3306..>4046)
                     /locus_tag="PLD05_15170"
     CDS             complement(3306..>4046)
                     /locus_tag="PLD05_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOP78826.1"
                     /translation="RKITAILTESGVKTDWIQWDVEHPTGYVTVDTSIPSQPRFTIHE
                     NVAWDYMELSSAWQNAVESAAAVCFGTLAQRHPVSRNTIRSLLNGQKEKRLLVFDVNL
                     RQHYFSRALIEEGLRLTRLVKLNQDEVRVLCQLLASPAGSEYEFCQHVLKRFGVDAVC
                     VTRGEQGCLIVDRSGTADVSGIPVNVADTVGAGDAFTAAWVVSRLLGWSLARQADFAN
                     RVGALVASLPGAMPPVKQEYEKILQTLG"
BASE COUNT          899 a         1195 c         1104 g          848 t
ORIGIN      
        1 cgtaacaagg tagccgtagg ggaacctgcg gctggatcac ctcctttcta aggatttcag
       61 cttacctcaa gctcgccttg agggacatgg agccggtccc agggcgttag ggattgcacc
      121 cacgctacca ttgctataat ccttgagcgg cggtcttctc aaagaccgcc gtttttcttt
      181 tcttgcggca atatcttagt gcttcgccgc gatgaagagc ctatcccata aactcccccc
      241 acgtgttcgg cgggagaagg gccgggggtg agggcggctc gaatcgctgc gggttttctc
      301 aatcgactcc gacgatcgct tgccctcacc ctaaccctct cccggaggga gagggagact
      361 ttttgggata ggctctaaag tagggggctt agcagcttcg ccaggtgaat ggtcaatttg
      421 cggacgggcc ggggttcttt ttcccgggcg ctatccaacg gacgcgacgc cgccaaatag
      481 cgaagttgca attccttaag ggcctgaacc gcctcggtgg aatagatcag caagttgact
      541 tcaaaattga gatgaaacga acggatgtca taatttgctg atccaaatag gcccaggcga
      601 ttgtcaatgg ttagtgtctt ggcgtgaaga aggccctctt ggaaaaaatg cacccggaca
      661 ccgtgctgag agagtcgccg ccagtgaaat tctcccgctg catcgacgag aggatggtca
      721 ctgcggagag gcaggacaag gtcaacttga acaccgcggt aggctgctaa tcgcaacgcc
      781 atcgtcaacg gctcgtcggg aacaaagtac ggtgacgtca aaataacccg ccgacgcgcg
      841 aggaaaatgg ccttcaccag aaggtcctga aagagctccg tcggcatgtc gggaccgctg
      901 ggaaggacct gcagaatggt ttggccgccc tcactgatga cggggaaata ccggggttca
      961 tcgagccact ggcctgtctc gtgaaaccaa tcctctagaa agagtaactg taagtggggg
     1021 acgctcggcc ccacaattcg ggccgtgata tcatgccagg gccccgcctt tcgacggcca
     1081 taggttggtt ccacaatgtt ttgggatccc gtgtaagcca cccttccatc aatgatagcc
     1141 aatttgcgat ggttacgaag gtccaaacgg gcaaaaggga ttcggtagag tcgaacagga
     1201 agggcagggt ggacctcgac accggctccg cggagtcggg gggcaagtcg tttgagcatg
     1261 gggcgcgaac cgacggcgtc aaccagcact cgacattgga cgccccgctg tgctgcccgc
     1321 accaaggcgt cggccacccg ccgtccaatt tcatcgtccc ggaaaataaa gtacaaaaga
     1381 tgcacgtggt cgttggccga atcgatctcc gccacgaggc ggtctatcgt caaaagtgga
     1441 tcacaaagaa atgacaattg gttttcagtc gtcaccggcc aacccgtcaa gcgaaaggca
     1501 aggtcgctga gtggctggag ttcgggatca aggcccccct ttcccatcgt cccggtctgg
     1561 agagaatcgt cggcgacatc agcttcgtcg gcgtcctctc gtgctggctc cccccaaagt
     1621 tcgctgctat cggactgata gtgagccccc acgggtggag cgttgacact ctccagtttt
     1681 gcccagcggc ttttgcggat ttccaaacgg cggcggccaa gccggttttc gcccaccagc
     1741 aggtagagaa gtaaacctgg cagtggggca aagaacacca ctgtcagcca cgcaaggcat
     1801 gtcgccggac gttcctttct catgacgatc accggcaaca tagctagccg gatgatccat
     1861 tctgagatcg tccagtatgt cgtccactcg ccgatggcca ttggtgctct tgaaagtgat
     1921 acgcagtcaa atcatgagtt ttcacaagga ctgcttcgat ttggttccag gcagctcatt
     1981 atcgttacac caatccattg tgatatactc ggcgtcgcga ggatgctatg ttccgagttg
     2041 tgtgtcaact gtctcttgtg tgccgatccg tccaaccagc agtgggcggc ggctggcagt
     2101 cgtttacaag tccgtaccaa cgaattggcg gttgtgcccc agttatggtc agttgcagct
     2161 caaggttttg tgctttccaa tcggggcaat ttcccattaa cggggactct acccgaaaac
     2221 aaggttgcaa cacaacggtt ggaaatttcg ggacaattat gggagttcga tcattgcctt
     2281 gatcaccccg tcgcgatact cggccacaag ctcgaaggcc tcttgaatcc ggttcaacgg
     2341 gtaccgatgg gtgatccacc acgtgggatt gatttggcct tgggccatcg cgtgcaacac
     2401 cggtttgaga cagtcccgct gtcgccggac caattgtagg gtgatttctt ttcttcgtgc
     2461 cagatgggca gggagcgaaa cgtgctcttc cggtgggatg cccacccata gtacgcggcc
     2521 tccgggtttt gcaagccgga tggcttgggt gacacatccc gcgtcgccgg aacactcaaa
     2581 cacgcaatcg actcccaacg gttcccgacg ctcaatagat gttgccacat cctcgcgggc
     2641 tgcattgccg gtccaatccg caccacaccg tgctgctgcc tccagccgtt catccaacag
     2701 atcggtgacg tacgccctac agccagccgt caattttacc cacgtcaaga cactcaatcc
     2761 gatcggccca cagcccaaaa tcgccacccg atccccactt tgaagctggg cgagccgcac
     2821 cgcgtacagc ccgatcgaga gcggctctgc cagtgctcca tgatctagtg tcaaacctgc
     2881 cgggagagga aaacaattgc tctcgggcag cgcgtaatac tcgcgcgctg ctccgggcgc
     2941 ctcaccagga caaccgagga attgcagccg tcggcaggtg ttcgaccggc ctgcccgaca
     3001 ctggtcacac tcattgcaga ccagcgccgg ctcaatcgcc accaggtcac ccaccttgac
     3061 ccgagtgact ttcggtccca cgtctaccac cgtgcccgca cattcatggc caacggaagc
     3121 cggatactga aggatttggt cgccaatgcg gccatgaaga aaataatgca cgtcagaacc
     3181 gcataccccc acgcgttcca catgcacaag cacgtcctgc ggacgctcga tttgggctgg
     3241 cggcgtctcc agtatgccca cctggcgcaa tcccgtaaaa aaggccgcct tcataccatc
     3301 tccattcacc ctaaagtttg gagaattttt tcgtactctt gcttcactgg tggcatggca
     3361 cccggcaaag atgccacgag ggcgcccacg cgattggcga agtccgcctg tcttgccaga
     3421 ctccatccca aaagacgcga cacgacccag gccgcggtaa atgcgtcgcc ggccccgacc
     3481 gtatccgcca cattcacagg aatccccgag acgtcggccg ttcccgaacg atccacgatc
     3541 aggcaaccct gctcgccccg ggtgacgcag acagcgtcca cgccaaacct tttaagcaca
     3601 tgctgacaaa actcgtactc actgcccgca ggactcgcca acaactgaca aaggacacgg
     3661 acttcatcct gattgagctt gaccaagcgg gtcaatctta gaccttcttc aatgagagcc
     3721 cgactgaaat agtgctggcg taggttgacg tcgaatacaa ggagccgttt ctctttttga
     3781 ccgtttaaga gtgaacggat cgtattgcgc gacacgggat gacgttgggc cagcgtgcca
     3841 aaacataccg ccgccgctga ctcgaccgcg ttctgccagg ctgacgacaa ctccatgtag
     3901 tcccaagcca cgttttcgtg gatggtaaac cttggttggc tcggaatcga ggtgtcaacg
     3961 gtgacatagc cggtcgggtg ttccacgtcc cactgaatcc aatctgtttt gacgccggat
     4021 tccgttagaa tggccgtgat ttttct
//
LOCUS       DAOSUT010000253         3997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9418_length_3997_cov_9.564181, whole genome shotgun sequence.
ACCESSION   DAOSUT010000253 DAOSUT010000000
VERSION     DAOSUT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9418_length_3997_cov_9.564181"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..951)
                     /gene="fliP"
                     /locus_tag="PLD05_15175"
     CDS             complement(61..951)
                     /gene="fliP"
                     /locus_tag="PLD05_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651465.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOP78827.1"
                     /translation="MIPGWFGFYNRHGVSGSSPVGRGDLLPAFKVFLAAIVIISFLAQ
                     SASEVRAEASPQSNRPVSLQVPQSVPLQPDQWTSPEGLSSALQILLLLTVLSLAPAII
                     LMTTSFIRVVVVLSLLRQALGTQTLPPSQVITGLAIFITLFVMSPVWMRVYDEAVVPY
                     QKREISLQEAWSRGQQPIRRFMSLQIERCNNSEDVWLFLRYAQLDEEPETYEDVPLQV
                     LIPAFMLSELKTAFLIGFQIYLPFLIIDMVVSTIMVSMGMLMMPPVLVSLPFKILLFV
                     LLDGWHLVVGMLMESFQPFV"
     gene            complement(1068..1916)
                     /locus_tag="PLD05_15180"
     CDS             complement(1068..1916)
                     /locus_tag="PLD05_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOP78828.1"
                     /translation="MPGLTWHRYLFLFFVAVIVMHPGVCRAQEATGRPWSGSGFVESQ
                     ATNGGMSFYQSANTNLSGFPAGDQVRQGEAGGDWPAAVPSNTPGFAFAGRQELTSPGR
                     EHPRTSATTPADGPVSDPVSPPQAGASSRASETSIPLRHPDAKGTSQTSQAKSPTACG
                     SATAPWMTTISALAIVLGVLFLFYWGMKRLGPKTNQILPAEAVELLGSTALSPRQRLC
                     LIRVGRKLVLVAMSSEGVEPLTEIDDPVEVDRLVGICASQRNGSATQVFRQVFSQYID
                     NAPRTG"
     gene            complement(2084..2779)
                     /gene="fliN"
                     /locus_tag="PLD05_15185"
     CDS             complement(2084..2779)
                     /gene="fliN"
                     /locus_tag="PLD05_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOP78829.1"
                     /translation="MSGDQLSQAEIENLLKAATQGAKPGESGTQAGGSGTAEPATGMS
                     SSNDSGGALSQDEIEKLLQQAGRGTGTVTPPDPKEKGSPSTAERSEQQPHAEIHPADL
                     DYLFHQAEQALASLENEGLPGLPPGVSAFQFTEFTGAPPSTQAATLDLLKEVELDVRI
                     ELGQTRMYLEDVLRLRKGTVVTLDKLAGDPVDIYVNGRLVARGEVLVLNDNFCIRVAE
                     LIAGKEPHVKDDA"
     gene            complement(2809..3450)
                     /locus_tag="PLD05_15190"
     CDS             complement(2809..3450)
                     /locus_tag="PLD05_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOP78830.1"
                     /translation="MANSSAPNAKAIPNSDPGNQSSTESPPSNRKGFSGHANRLKLIG
                     FVLGLVTVEAVAAYFILPTSSDAKSPTQEGVAEAAGNAGAPSPISVPETQLDPTQGLG
                     NTPGADDVEVDLGQFSITAYQPTSQTTLRIDFHLWGTVDRSLDQEFQAAWAKNINRLR
                     DQIIVIVRSADLSDLTDAGLGLIKRKILEKVNHTLGKPYLRTVVFSEYSFLEQ"
     gene            complement(3761..>3997)
                     /locus_tag="PLD05_15195"
     CDS             complement(3761..>3997)
                     /locus_tag="PLD05_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOP78831.1"
                     /translation="APGSPFRSHWDLAYAECLKVMEGLIAAGIEPERIRISIAAANEP
                     LWTDPDPEKRPQNARVEVSLLNELARDLKGSSTP"
BASE COUNT          924 a         1116 c         1088 g          869 t
ORIGIN      
        1 cactcacgaa acagatccac ggcggcctgt ggttccatgg ctctcggcga tgttttcctt
       61 tcacacaaac ggttgaaaac tctccatgag catgccgacc acgagatgcc atccatcaag
      121 caggacgaac aaaagtattt tgaagggcag tgagaccaac acgggtggca tcatgagcat
      181 gcccatcgag accatgattg tggatactac catgtcaatg atcagaaatg gcagatatat
      241 ctgaaatccg ataagaaaag ccgtcttgag ctcgctaagc ataaacgccg gaatcaggac
      301 ctgtagggga acatcctcgt aggtttcagg ttcctcgtcc aactgggcgt agcggagaaa
      361 gagccatacg tcctcactat tgttgcaacg ttcaatttgc agactcatga agcgccgaat
      421 gggttgctgg ccgcgcgacc atgcttcctg aaggctgatc tccctctttt ggtacggcac
      481 aacggcttca tcatagaccc gcatccaaac gggcgacatg acgaagagcg tgataaaaat
      541 cgctaggccc gtgatgactt ggcttggcgg cagtgtctgc gtccccaagg cttgtcgcag
      601 aaggctgagg acgaccacca cgcggataaa tgatgtcgtc atcagaatga tggcgggcgc
      661 cagactaagg actgtcagaa ggagcaaaat ctggagggca ctcgagaggc cctccgggct
      721 ggtccactgg tcgggttgca atggtacaga ttgaggaact tgcaacgaga cggggcgatt
      781 tgattgaggc gatgcctcgg ccctgacctc tgatgctgac tgggccaaaa aggagatgat
      841 aacgattgct gcgagaaaaa ccttaaaggc gggcaggagg tcgccacgtc ccacgggcga
      901 ggaaccactt acgccgtgcc ggttatagaa accgaaccac cccggaatca tcgcatgctt
      961 cccatccttg ggtatctcat gctctccaca caactccaat cgtctttgtc agtaaaaccc
     1021 ttgtgtgaca ggtacagtcc gccctggagc gtctctccag ccaatgccta accagtcctc
     1081 ggggcgttat caatatactg ggaaaaaact tgacgaaaga cttgcgtggc actgccgttc
     1141 cgttgcgacg cgcagatacc caccagtcga tcgacttcca cagggtcatc gatctcggtt
     1201 aggggttcaa caccctccga actcatcgcc accaacacaa gttttcggcc aacgcgaatg
     1261 agacacaacc tctgccgcgg cgatagcgca gttgacccca gtaattcgac ggcttcagca
     1321 gggagtatct gattggtctt tggccctagt cgcttcatgc cccaataaaa aaggaacaat
     1381 acaccaagga caatggcaag ggcactaatt gtggtcatcc aaggtgccgt tgcagatcca
     1441 caagcggttg gggatttggc ctgcgacgtc tgggaagtcc cctttgcgtc ggggtgccgc
     1501 aaaggaatcg acgtctctga cgcgcgtgag cttgcaccag cctgcggagg tgacacggga
     1561 tcggacacag ggccgtctgc tggagttgtc gcggaggtac gtggatgttc tctgccaggt
     1621 gaagtcaatt cctgtcttcc cgcgaaggca aagcccggcg tgttggaggg aacagccgcc
     1681 ggccaatctc cgcctgcctc cccctgcctc acctgatcac cggcgggaaa gccgctcaag
     1741 ttcgtgttgg ccgattggta aaaactcatt cccccattcg tggcctggga ttccacaaac
     1801 cccgaccctg accaaggtcg tcccgtcgcc tcttgagcgc gacacacccc aggatgcatg
     1861 acaattacgg caacgaaaaa caagaacagg taacgatgcc aggtcaaccc gggcaagaaa
     1921 gacggcatcc ttatgagggg ctttgaggaa gaaacgggtt cccttcggta tgccttggca
     1981 acaccatgtc gaggcccctt ggaataagag gcccaaaagg caccgttgtc gttaaccagc
     2041 atccgtgctg ccacggaaaa accccttgtc gtcttatcga ggattaagcg tcatctttca
     2101 catgcggttc tttcccggcg atgagctccg ccacgcgaat gcagaagttg tcgttgagaa
     2161 cgaggacttc gccgcgggca acaagccgtc cgttgacata aatatccaca gggtcacccg
     2221 ccagcttgtc caacgtcacc acagttccct tccgcagacg cagcacgtcc tccaaataca
     2281 tccgcgtttg tcccaattcg atgcgaacat caagttcgac ctctttcaaa aggtccaggg
     2341 tagcggcctg ggttgaaggt ggggcaccgg tgaactccgt gaattggaag gccgaaactc
     2401 ccggcggaag cccaggcaga ccctcgtttt ccagcgatgc cagggcctgc tcggcctgat
     2461 gaaagagata gtcaaggtcg gccgggtgaa tctcggcgtg cggctgttgt tcagatctct
     2521 cagccgttga aggactaccc ttttctttgg gatcgggagg ggtgaccgtt ccggtcccac
     2581 gtccggcctg ctggagtaat ttctcgatct cgtcctgact gagtgctccc ccggaatcat
     2641 tgctggaaga cattcccgtg gccggttccg cggtgcccga acctccggcc tgggtgccag
     2701 attcccctgg ctttgctcct tgcgtagccg ccttgagcaa gttttcaatt tcggcctgtg
     2761 acagttgatc gccagacatg ggactccctt ccctcgccaa cttagccgtc actgttctaa
     2821 gaaggaatac tcactaaaca caaccgttcg tagatacggc ttccctagcg tatggttaac
     2881 tttctctaaa atcttccttt tgatcaaccc caaacctgcg tccgtgaggt ccgataggtc
     2941 ggcactgcgg acaatcacga tgatttggtc acgcaaccgg ttgatattct tcgcccaagc
     3001 cgcttggaat tcctgatcta gagacctgtc aaccgtcccc cataagtgga agtcgatccg
     3061 cagtgtggtt tgggacgttg gctggtaggc cgttatgcta aattgaccca ggtccacttc
     3121 gacatcgtca gcacctgggg tattacccaa cccctgagta gggtccaact gagtttcggg
     3181 aaccgaaatc ggcgatggcg ccccggcatt ccctgcggct tcggcaaccc cctcctgagt
     3241 aggagatttt gcgtcactgg aggttggcaa aatgaagtac gcagccacgg cctccacggt
     3301 gactagcccg aggacaaatc caataagttt cagacggttt gcgtgaccgc taaagccttt
     3361 cctatttgat ggggggcttt cagtggagct ttgattaccg ggatcactgt ttgggatagc
     3421 cttcgcatta ggtgcagaac tatttgccat gctcgccctt tccgaattgc gcggaaaccg
     3481 aatagtcgct gtgacaagcc aaagcggggg tgaccaagta ccaccgcctc aattcaaata
     3541 taggtcatgc ttttgcgcgg ggtaaaatgc gcatgccggg aaatatgcac cgcttaagga
     3601 ggtttcacat acatgagaag cagcataaat tagagaactc aatctggctg ggtacctttc
     3661 aacggggcac agtgaaccgg gctattgatc gggctaccgg ccccatcgtc gagaaaaggt
     3721 ttggctttgt ggggattctt acggcgaaag acgatcgcca tcagggcgtg gaagagccct
     3781 tcaagtctct tgccagttcg ttgaggaggg aaacttcgac ccttgcattt tgtggccgct
     3841 tttcgggatc gggatcggtc caaagcggct cattcgcagc tgctattgaa atacggattc
     3901 gctcaggctc gatgcccgcc gctatcaagc cctccatgac cttgaggcat tctgcgtagg
     3961 caaggtccca gtgagagcga aaaggcgatc ccggggc
//
LOCUS       DAOSUT010000254         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9426_length_3996_cov_8.714793, whole genome shotgun sequence.
ACCESSION   DAOSUT010000254 DAOSUT010000000
VERSION     DAOSUT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9426_length_3996_cov_8.714793"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(539..>2064)
                     /locus_tag="PLD05_15200"
     CDS             complement(539..>2064)
                     /locus_tag="PLD05_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOP78832.1"
                     /translation="KKAVAYLQPYMEAEKATSGQNGRYRGTIVLATVKGDVHDIGKNI
                     VGIVLACNNYRIIDLGVMVPCETILDTAEKENADIIGLSGLITPSLEEMVHVAREMNR
                     RGMQQPLLIGGATTSAKHTAVKIAPEYDGITVYVPDASQSVSVVEQLMNPGQRHTFAE
                     SVRREQEKLAEAYHQRRTVNLVSYEEAVKKRFTCDWQTVDIPRPSFLGRQPVDVTVDV
                     LIPYIDWTPFFIAWELPGTYPKIFDDPRFGPQARDLFRDAQKMLETIAAKQFLRPRGV
                     YGFWPANSQGDDILLFTDEARSHLLARVYTLRQQWRRQGQEVFFALADFIAPLESGRI
                     DYLGAFAVTSGHEAVKLARHYETQHDDYRAIMVKALADRLAEAFAEWLHEKARRAWGY
                     GQDEHLTNEDLIKERYRGIRPAPGYPACPDHTEKITLFALLDATTITGIRLSETLAMD
                     PPASVCGWYFAHPQARYFAVDRITRDQVEAYAQRKGLPIKEVERWLAANLAYDPAES"
     assembly_gap    2065..2164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2165..3993)
                     /locus_tag="PLD05_15205"
     CDS             complement(<2165..3993)
                     /locus_tag="PLD05_15205"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOP78833.1"
                     /translation="MGPTNRTASLSPDVNRPGYRAVTFDDLYVAYGEQAASVIAAGAD
                     ILLVETVFDTLNLKACLMALEDLFDRLGRRLPVMVSVTITDRSGRTLSGQTLEAFWIS
                     ISHAPLFSVGINCALGPELMRPFVEELSSLAPIYTSCHPNAGLPNEFGGYDETPEQMA
                     KTLRQFAQAGWLNIVGGCCGTTPEHIRAIAEAVRDCPPRTPPEVPRFTRLAGLEPLVI
                     RPDSNFILIGERTNVAGSRRFARLIREGRYDEAVAIARQQVEGGANILDVNMDDALLD
                     GEQAMREFLNRLAAEPDITRIPIMIDSSKWSILEAGLKCLQGKPVVNSISLKEGEQAF
                     LERARRIRRYGAAVVVMMFDEEGQAVTVEHKVRIAQRAYRLLTEQAGFPPEDIIIDAN
                     ILTVGTGIEEHNRYAINFIEAVRQIKQLCPGVKTSGGVSNISFSFRSNETVRRAMHSA
                     FLYHAIRAGLDMAIVNAGQLDVYEEIDPTLRELVEDVLFDRRPDATERLIRFAESSAV
                     SSQEQQEKTQQRWREAPVTERLKHALVHGIDEFIEQDVEEIRHQFPSALAIIEGPLMD
                     GMRIVGDLFGEGKMFLPQVVKSARVMKKAVAYLQPYMEAEKATS"
BASE COUNT          772 a         1126 c         1159 g          839 t
ORIGIN      
        1 gcaaaaaact cgcgtcaaac ggcctttaaa cggcccaaaa tcgtcagttt tttgcgagtt
       61 tttcatcgct ctttgcccca catcatacca ccctgcccct ggcgttgcaa gaccccgcca
      121 cgagtaaatc ctgccgcctg ttgaagccta tcccataatc tcccctcgcc cgtttagcgg
      181 gagaggggtc gggggtgagg gtggctcgaa tcgctgtggt tttctcaatc gattatgagg
      241 ttcgcttgcc ctcaccccaa ccctctccca gagggagagg gggctgggag gtcatcgtcc
      301 ccgatgacca aatcgccgga caagacggat cctcggttga gacaagccgt caacaccggg
      361 acggacaata acaccggcaa gatgcccgtg ccatgcacag gacgtggggc aggcacgggg
      421 gcctgcccct acacctttcg atgggcgcgt cgatcgtagg ggcaaccccc tgtggttgcc
      481 cccgtttggc cccagaaaca cccgggaggg tcgccggcct cggcgaccaa atcatcgctt
      541 agctttccgc ggggtcgtat gccaggtttg ctgctagcca gcgttcgact tctttgatgg
      601 gcaggccttt tcgctgggcg taggcctcca cttgatcccg cgtgatccga tcgaccgcaa
      661 aatagcgggc ttggggatgg gcaaaatacc acccacacac cgaggcgggc gggtccatgg
      721 caagggtttc ggagaggcgt attccggtga tcgttgtggc atctaggagt gcaaagagtg
      781 tgatcttctc cgtatgatcc ggacaggcag ggtagcccgg cgccggacgg atgccccgat
      841 aacgctcctt gatcaaatcc tcgttagtca gatgttcgtc ttgaccatag ccccaggcgc
      901 ggcgggcctt ttcgtgcagc cattcggcaa acgcctcggc aaggcggtcg gccagggcct
      961 tgaccatgat cgcccgatag tcgtcgtgct gggtctcata gtggcgggcg agtttcaccg
     1021 cttcgtgccc agaggtgacg gcaaaggccc ccaggtaatc gattcttccg ctttccaacg
     1081 gggcgatgaa atcggcgagg gcgaaaaaga cctcctgtcc ttgccgtctc cactgctggc
     1141 ggagcgtgta aacccgcgcg aggagatggc tgcgggcctc atcggtaaac agcaggatat
     1201 catctccctg gctattggcc ggccaaaagc catagacacc tcgcggcctg aggaattgct
     1261 tggcggcgat ggtttccagc attttctggg catcccgaaa cagatcgcgg gcttggggac
     1321 caaatcgcgg atcatcaaaa atcttgggat acgtccccgg aagttcccag gcgatgaaaa
     1381 acggtgtcca gtcgatatag gggatgagga catccacggt gacatccacc ggttgcctac
     1441 ctagaaaact tggccgggga atatccacgg tttgccagtc gcaagtgaag cgttttttga
     1501 cggcctcctc gtaagagaca agattgaccg ttctgcgttg atggtaagcc tccgccaatt
     1561 tttcctgctc acggcggacg gactcggcaa aggtgtgccg ctggccgggg ttcatcagtt
     1621 gttccaccac cgaaacactc tgtgaggcgt ccggtacata aacggtgatg ccgtcatatt
     1681 cgggggcgat tttgacggcc gtatgcttgg cgcttgtcgt ggccccacca atgagcaagg
     1741 gctgctgcat cccacgacgg ttcatttcgc gtgccacgtg aaccatttcc tccaaacttg
     1801 gcgtaatgag gccggacaag ccgatgatgt cggcattttc cttttcggcc gtgtccaaga
     1861 tggtctcgca ggggaccata acgcccaggt cgatgatgcg gtaattgttg caggccaaga
     1921 cgattcccac gatgttcttc ccgatatcat ggacatctcc tttgacggtg gccaagacga
     1981 tcgtcccccg atatcggccg ttttggcccg aggtggcctt ttcggcctcc atgtagggct
     2041 gaagataggc gacggccttt ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnngaggtg gccttttcgg cctccatgta gggctgaaga taggcgacgg cctttttcat
     2221 gacgcgggcc gatttgacga cctggggtaa aaacatcttt ccttcgccga agaggtcccc
     2281 cacgatccgc atgccgtcca tgagaggccc ttcaatgatg gccaaggcgc tggggaattg
     2341 atggcgtatc tcttccacat cctgttcgat aaattcatca atgccatgga ccaaggcgtg
     2401 tttgaggcgt tcggttacgg gggcctctcg ccagcgttgc tgcgtttttt cttgctgttc
     2461 ctggctcgat acggcggaac tctcggcaaa gcggatgagt cgttcggtgg cgtcggggcg
     2521 tctgtcaaag aggacgtcct ccaccaattc gcggagcgtg gggtcgattt cctcataaac
     2581 atccagttgc cccgcgttga cgattgccat atccagcccc gcccgaatgg catgataaag
     2641 aaaggcggaa tgcattgccc gccggacggt ctcattggag cgaaagctga aggatatgtt
     2701 acttactccg cccgatgttt tgacgccggg acaaagttgc ttgatttggc ggactgcctc
     2761 gatgaaattg atggcgtaac ggttatgctc ctcgatgccc gttcccaccg tcaggatgtt
     2821 cgcgtcaatg atgatatcct ccgggggaaa gcccgcctgc tcggtcaaca ggcgataggc
     2881 ccgttgggcg atccgcactt tgtgctctac cgtgacggcc tgtccctcct cgtcgaacat
     2941 catcacgacg acggcggcac cgtagcggcg gatacgtctt gcccgttcta agaaggcctg
     3001 ttccccttcc ttaaggctga tggaattgac gaccggcttg ccctgaagac actttagccc
     3061 ggcttccagg attgaccact tggagctgtc aatcatgata gggattcgcg tgatatccgg
     3121 ctcggccgcc agacgattga ggaattccct catagcctgc tcgccatcca acagggcatc
     3181 atccatgttg acatcgagga tgttggcccc gccttccacc tgttgccggg cgatggccac
     3241 ggcctcgtcg tagcgtccct ccctaatcag tcgggcgaag cggcgggaac ccgccacgtt
     3301 ggtccgctcg ccaatgagga tgaaattact gtcgggacgg atgacgagcg gttctaaacc
     3361 ggccaggcgg gtaaatcttg ggacctcggg cggagtccgc ggcggacaat cccgcaccgc
     3421 ttcggcgatg gcccggatgt gttctggtgt cgtcccgcaa cacccgccca caatattcaa
     3481 ccaaccggcc tgggcaaact ggcgtaaggt cttggccatc tgttcgggcg tttcatcgta
     3541 accgccgaat tcgttgggta agccggcgtt ggggtggcaa ctggtgtaga tcggcgcaag
     3601 tgaggataac tcttccacaa acggccgcat cagttccggc cccagggcgc agttgatccc
     3661 cacgctgaaa agaggggcat gcgagatgga aatccagaag gcctctagcg tttgcccgga
     3721 aagcgttcgg ccactgcggt ccgtgatcgt tacggagacc atcaccggca agcggcgtcc
     3781 caaacgatcg aagaggtctt ccagggccat gagacaggcc ttcaggttga gcgtatcgaa
     3841 gacggtctcg accaggagga tatcggcccc cgcggcgatg accgatgctg cctgctcgcc
     3901 gtaagcaacg taaagatcgt cgaaggtcac ggcccgataa cctgggcggt ttacgtcggg
     3961 cgagagggat gccgtgcgat tggtgggccc aatgga
//
LOCUS       DAOSUT010000255         3931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9582_length_3931_cov_7.495098, whole genome shotgun sequence.
ACCESSION   DAOSUT010000255 DAOSUT010000000
VERSION     DAOSUT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_3931_cov_7.495098"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..472)
                     /locus_tag="PLD05_15210"
     CDS             complement(56..472)
                     /locus_tag="PLD05_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78834.1"
                     /translation="MVFFTQKRIPEVQSFLLKFVNNHSDALDSIALHKRRETRARLTV
                     VVLVVPFENGKAQIHEAFTAISKEFSTSGMSLILPEPRGFDQLAIGLKFLGGVKFLLA
                     RAVHLTPMGGGFYQLGLQFMEMLHPADLPELQNVTF"
     gene            complement(1410..1844)
                     /locus_tag="PLD05_15215"
     CDS             complement(1410..1844)
                     /locus_tag="PLD05_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78835.1"
                     /translation="MTQRERILAISLAIFLGALTLQGCQKSVGVTVSGKVVYSGQPVT
                     TGYVTFAPADGKGPSVGGPIVDGNYKVVGVPPGEKVVSVVGGGEIQFPKTSEEMARMT
                     AKGQPLERSRQIPANAKGNNQKIVITKESKQTVDLSLEAPGP"
     gene            complement(1889..2911)
                     /locus_tag="PLD05_15220"
     CDS             complement(1889..2911)
                     /locus_tag="PLD05_15220"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOP78836.1"
                     /translation="MRYCRRGFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCV
                     NNIKQLVLACHNFHDRSKEFPYARKYDVWDTYTWTQLILPFIEQGAVYDKYWTLPLRP
                     YSQTHPGPVTPAGDDAKLREARHTPIAAFMCPSDGGPYGNELSSNTWGFYRGNYRGCV
                     GSGDMYGQDVQDGTTGPWKPGVFAVKKDQSFDTGATIPARGAQIAQILDGTSNTAMIS
                     EGISPRCSNWGGPIGSIIYGNMGGALYSHAVTPNSTSPDRIIGPCPQNQGDTEYTAPC
                     VSIGANVAWTRSAERAFAGARSRHPGGVNVGMADGAVKFFANTVDLAVWRYLATRAGQ
                     ETVSAQ"
     gene            3399..>3931
                     /locus_tag="PLD05_15225"
     CDS             3399..>3931
                     /locus_tag="PLD05_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78837.1"
                     /translation="MCEHCTRREFLGTGVLSALAASSTWNYTWASQAETAQPLGKVRV
                     GVLFTGSPGPADRGWNADEGHFEKMQTILSDVQNKLSNVELIIGQSQNPQQTAAFLDQ
                     IGEGVPVLAINLNIFALTRLVGPLLERRHPMAVFSLPASGHDWMYPPRWQRAGHRVTL
                     FATSDMNEIEHAIRLLRV"
BASE COUNT          870 a         1070 c         1038 g          953 t
ORIGIN      
        1 gagccgcctg ccccctgtgg caacgggcaa ggtctccgag accttgcccg tttttctaaa
       61 aggttacatt ctggagctcg ggcaagtccg ctggatggag catttccatg aactgtagtc
      121 ccagttgata gaatccaccg cccatgggtg tcaaatggac tgcgcgggct aacaggaatt
      181 tgaccccgcc taggaacttc agcccaatgg ctaactgatc gaagccgcgc ggctcgggaa
      241 gaatcaagga catgcccgat gtggagaact ctttgctgat ggcggtaaaa gcctcatgga
      301 tttgggcttt accattttca aaggggacga cgaggacgac gaccgtcaag cgggcccgtg
      361 tttcccgccg cttgtggagt gcgatcgagt ccagggcatc cgaatgattg ttaacgaact
      421 tcaacaggaa gctctgaact tccggtatgc gtttttgcgt gaaaaagacc atcgttttgc
      481 ctcggtcggt aattgcagca agagatgacc tttggaatag gtcatggccc agtcactaat
      541 tcacatttca gttctaactg gacttttgca aattgggggc acaaggccct ctagcccccg
      601 aggagagggt tagcgtgacg gcaattagct gcgaaagcgg tttgctttgt ttgcctgatt
      661 taaccaagcc ctcacccccg cccctctccc agagggagag gggagcagtt tgcaaaatcc
      721 agttctaagg aaaccatagc gtttccaaga ccccctggcg ttgcggacgg gtcaattctg
      781 gtcaccagtg ggccttcagg ctgtcatctc gttccgtttg ggtggaatag tcgggttttt
      841 ggggagcggg ggcacttttc cggaggagta tcctcttgcg gagtctattc acgtgttgcg
      901 gtatcgtagg gcggggccgc cagcggcagg tgaggcggcc ctatagccaa cggttcgccg
      961 cgctaccctg agaggggaca gaaccattga ccgcgttgcc tagtcatcac aggccaggtc
     1021 atttgtcaca gactgtttgg cattgggtcg gttttgctgg aacaaattga ggtttttgcc
     1081 tggacttcac ccacgcttgc cctgagaccc tcctgcaacg attgtcgtac ttgagcgagc
     1141 tagttattgt tgcgtaagag gtttccgggc aacgccggtc ccaccgcgca cctagcgaac
     1201 aacgcacccg gctgctagcc agaagcccga cttttcgcaa tggctctcac ccctctaacc
     1261 gcgttccggc acaatcacaa catggttgcg caccagcagc aacaaccagg gcgaatcgct
     1321 acaaacctag ccgcggtgat tgagacaaaa agtgccgttt gaaggtggca tctacaacag
     1381 ccttggaccg tctttaatgg cctagtctat tagggccccg gggcttctaa cgaaagatca
     1441 actgtttgct tcgactcctt tgttataacg atcttctgat tattgccttt ggcgtttgcg
     1501 gggatttgtc tcgaccgctc aagtggttgc ccttttgcgg tcatccgtgc catctcttca
     1561 cttgtttttg gaaactgaat ttcaccaccc ccaaccacgg acacaacttt ctccccgggc
     1621 ggaaccccga ccaccttgta gtttccatcc acgatagggc caccgacact tggtcctttt
     1681 ccgtctgccg gggcgaaggt gacgtagcct gtggtaacgg gctgtccgga gtaaacaact
     1741 tttcctgaga ctgttacgcc aactgacttt tgacagcctt ggagagtcaa agccccaagg
     1801 aaaatcgcca ggcttatcgc aagaattctc tctctttgtg tcatatgctg tccttcgaga
     1861 ttacttagtt tcgtaggttg gccatgtgct actgagcaga tacagtttct tgtccggcgc
     1921 gtgtcgctag ataccgccag accgccaaat ccacggtatt tgcgaaaaac tttaccgccc
     1981 cgtcagccat tccgacgttc actccacccg gatgacggct tcgtgcgccg gcaaatgccc
     2041 tttcagcgct tcttgtccat gctacatttg caccaatgct tacgcagggg gccgtatatt
     2101 cagtatcacc ttgattttgt gggcatggcc cgatgattcg gtccggcgag gtggaattgg
     2161 gggtcaccgc gtggctgtag agtgccccgc ccatattgcc ataaattatg cttcctattg
     2221 gccctcccca gttagagcat cgaggggaaa tcccttccga aatcatagct gtattcgatg
     2281 taccatcaag aatctgcgca atttgtgcac ccctcgcagg gatggtagcg ccagtatcaa
     2341 agctttggtc ttttttcacc gcgaacacac ccggtttcca tgggccggtt gttccatcct
     2401 gcacatcttg cccgtacatg tcccccgaac caacacaacc acgataattc ccacgataga
     2461 agccccatgt gttgctcgat aactcgttgc cgtaaggacc gccatcgctt ggacacatga
     2521 aagccgcaat cggagtgtgt cgagcctcac gaagcttagc gtcatcgccg gcgggcgtta
     2581 ccggtccagg atgagtttgc gagtaaggac gcaacggcaa ggtccaatat ttgtcataaa
     2641 cagcgccttg ctcgataaac ggcaggatca actgagtcca ggtgtacgta tcccaaacat
     2701 cgtatttcct cgcataagga aattccttcg accgatcatg aaaattgtgg caagccaaaa
     2761 ctaactgctt gatgttgtta acacactgcg atcgccgtgc tgcctcccgt gccgcctgga
     2821 ccgctggcaa caatagggca atcaggatac caatgatcgc aatgacaacc aacaactcga
     2881 ccagagtaaa ccctcgccga cagtagcgca taccgaagcc tcctggacaa gaaccgttga
     2941 gacggaccgc catttctaaa cttcatttta gtgaggacaa caaaacgttg caattcactt
     3001 ttgcgcaaat ttatatgcac ttttgcgcac acgctccaac tgtcaatgac ctcccgagaa
     3061 gcccaatatt agtctcggcg aggatatgtc tttccttccc ttgagaggaa gacttttagg
     3121 aggtgcgttg gttacgcacg cggtcattac agactcagtt ggatggaaag aaagtcacgg
     3181 accacagagc gctgaacgta cagaactctt cccgtttctt gacttcgttc cgacaggctt
     3241 gaggagagct gggacagaat cccggattcg tcgattggcc gtccgccagt agaagttcgc
     3301 atcgaaacca ttgacaaccc tctgccgata agacatcatc tatgcgggtg acttggaact
     3361 ccctattcca ttttcccagt tttgaggaga tgttagccat gtgtgaacat tgtacgcggc
     3421 gggagttttt ggggactggc gttctcagcg ccttggcggc gtccagcacc tggaattaca
     3481 cgtgggcctc gcaggcggag acggcacaac cactcggcaa ggtgcgtgtt ggcgtgctat
     3541 ttacgggttc accgggtccg gctgatcggg gatggaacgc cgacgaggga cattttgaga
     3601 agatgcagac gattctttct gacgtccaga ataagctcag taatgtagag ctgatcattg
     3661 ggcagtcaca gaatccgcag caaacagccg cttttctcga tcaaatcggg gaaggcgtgc
     3721 cggttttggc gatcaacctc aacattttcg cgctcacgcg gttggtcggg ccattgctgg
     3781 aacggcgcca ccctatggcg gttttctctt tgcctgcgag tggtcacgat tggatgtatc
     3841 cgccgcggtg gcagcgggcc gggcatcggg ttacgctttt tgctacaagt gacatgaacg
     3901 agattgagca tgccatccgc ttgttgcgtg t
//
LOCUS       DAOSUT010000256         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9623_length_3914_cov_7.094066, whole genome shotgun sequence.
ACCESSION   DAOSUT010000256 DAOSUT010000000
VERSION     DAOSUT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9623_length_3914_cov_7.094066"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLD05_15230"
     CDS             <1..957
                     /locus_tag="PLD05_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOP78838.1"
                     /translation="SRIVMGCWGLAGDQTWGPQDEGEAIATIHAAIDAGINFFDNAEL
                     YGNGIAEEILGKALSGRRQQVFIASKFNWEHARREQVIAACERSLKRLRTDYIDLYQI
                     HWANWDVPFEETWEALEFLRTKGKIRAIGVCNFGPRDLAAILQCGRPSTNQLPYGPLF
                     RAIEFEIVPACIGAGVGILCYSPLTIGLLTGKYKSADEVPPGRARTRHFSSKRPLTRH
                     GEPGCEEATFQAIAQLQALAAELGMSVTQLALAWLLHRPGVTCVINGMRHPEQARENA
                     IAGDVDLTPDVISRIESITDPIKERLGPNPDMWEGTDRSRFR"
     gene            complement(1054..1806)
                     /locus_tag="PLD05_15235"
     CDS             complement(1054..1806)
                     /locus_tag="PLD05_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP78839.1"
                     /translation="MDVLVLQSGSRGNSVYVESNGIAVLIDAGISARQVEARLSQYGK
                     SLDAVRAVVISHRHRDHIGHVGVYQRRLRLPVFMTEDGCDVVPDLDETMCHFFCPDQQ
                     LQFEHLRVEPVLTPHDAPPSLAFVVSDEKHRLAILTDLGHPFSQLPAILASVDAAILE
                     SNYDPVLLSEGDYPPWLQDRIRGPGGHLSNKEAALLVKNHGKHLRWICLAHLSQHNNT
                     PEVAYQTYRRVVGNGEPFIADYFSATPLPSLR"
     gene            complement(1924..3240)
                     /locus_tag="PLD05_15240"
     CDS             complement(1924..3240)
                     /locus_tag="PLD05_15240"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOP78840.1"
                     /translation="MGKRRIGFLGTLLVGTLLITVGHAILANDAVNPVPRSDQWWMQR
                     HQSMNDRVKQGNVDLIFIGDSITHGWEGAGKEIWEAYYGHRNAVNLGIGGDRTQHVLW
                     RLDNGNIDGIQPKLAVIMIGTNNAAPNQPEETAEGIKAIVQKLRERLPKTKILLLAIF
                     PRGETPEDPLRQKNEQVNKIIAQFADGEMVKFVDIGPAFLTIDGILPKDIMPDLLHPN
                     TRGYSIWAAAIEPYVAEVLGPLPPPQAEPDAEGFVPLFDGKTIAGWRKAGGDATYWTE
                     DGCIVGKVGPGPNTFLCTEKTFSDFILKVEAKFDVPGNSGIQVRSHQRPDTGRVYGYQ
                     CEIDPSDRSWSAGIYDEARRGWLFTLENKEDARKAFKRDDWNEFVIEAKGRHLKTYLN
                     GVPCADYVDESDQADLEGFIALQVHRGKQGQIRWRNIRIKVLDKAE"
BASE COUNT          902 a         1074 c         1033 g          905 t
ORIGIN      
        1 tcccgcattg tgatgggctg ctggggattg gctggtgacc agacttgggg acctcaagat
       61 gaaggtgagg ccattgccac catccatgcg gccatcgatg cggggattaa cttctttgat
      121 aacgcagagc tatacggaaa tggcatcgcc gaggaaattc tcggcaaagc cttgtcaggg
      181 cgtcgtcagc aagtattcat cgcgtcaaag ttcaattggg aacatgccag acgtgaacag
      241 gtcattgctg cctgcgaacg cagtttgaag cggctcagaa cggattatat cgatctttat
      301 cagattcact gggccaactg ggatgtgccg ttcgaagaga cgtgggaggc tctcgagttt
      361 cttcgaacaa agggaaagat tcgcgctatc ggggtatgca attttggacc gcgggacttg
      421 gccgccatcc ttcagtgtgg gcgtccgtcc acgaatcaat taccctatgg tccactattt
      481 cgagcgatcg aatttgagat cgtgcccgca tgcattggtg ccggtgtggg gattctttgt
      541 tacagcccgc tgacgattgg acttctcaca ggtaaataca agtccgccga tgaagtgcct
      601 cccggtcgtg cacggacccg acatttttcc tcgaaaagac ccttgacgcg acacggtgag
      661 ccgggttgtg aagaagcaac ctttcaggcc attgcccagc tccaggccct tgcggccgaa
      721 ttgggaatga gcgtgaccca attggccctc gcttggctgc tccaccggcc cggtgtcacg
      781 tgtgtcatca atgggatgcg gcaccccgag caagctcgcg aaaatgccat tgccggtgac
      841 gttgacctga caccggatgt gatcagtcgc attgaatcca tcaccgatcc gattaaggag
      901 cggttaggtc ccaatcccga tatgtgggag ggcacagatc gctcccgttt tcggtgattt
      961 tgaagctacc gcggggcacc aacgctgcca cgtgcccgat tcaaagttca cctcgggggt
     1021 aagcaagtct tctttgtgct cgggttcgcc atattaacgg agtgaaggta atggcgttgc
     1081 gctaaaataa tcggcgatga agggttcccc gttcccaacc accctgcggt acgtctgata
     1141 tgcgacctct ggcgtattgt tgtgttgaga aagatgagca aggcagatcc atcgcaggtg
     1201 tttgccatgg tttttgacaa gcaaggcggc ctctttgttg gagaggtgac ctcccggccc
     1261 gcgaatacgg tcttgaagcc atgggggata gtcgccttcg gaaagcagaa ccggatcgta
     1321 gttgctttcc aaaatcgctg catccacgct cgccaggata gccggtagtt gcgaaaaggg
     1381 atgtcccaaa tcggtcagga tggccaagcg atgcttttcg tctgacacaa cgaaggcgag
     1441 agatggaggc gcgtcatgcg gtgtcaaaac cggttccaca cgcaagtgct caaattgcaa
     1501 ctgctgatcc ggacaaaaga aatgacacat tgtctcatcg agatcaggaa ccacgtcgca
     1561 accatcttcc gtcatgaaaa ccggtaggcg aagtcttctc tggtaaacac cgacgtgccc
     1621 gatgtggtct cgatgacggt gagagatcac gacagcccgt accgcgtcaa ggctcttgcc
     1681 atactgggaa agacgagcct cgacctgccg ggcgctaatt cccgcgtcaa tcaaaacggc
     1741 aataccgttg gattccacat aaacggaatt gccgcggctg ccggactgta aaacaaggac
     1801 gtccatgaag cctgtctttc tccttggtag aaaaaaataa ccatctcgct agatgtgggc
     1861 tcatcaccca cacctagacg agacttgatg aaaggctcct ctaaactgct gccgaggccg
     1921 gaatcattca gccttgtcga gcaccttgat gcgtatattc cgccagcgga tttggccttg
     1981 cttaccgcgg tggacctgga gagcgataaa gccttccaaa tccgcctgat cactttcatc
     2041 gacgtaatcg gcacagggca cgccattcag atacgtcttg aggtgacgcc ctttggcctc
     2101 gatgacgaat tcgttccagt catcgcgctt gaatgccttg cgagcatctt ctttgttctc
     2161 aagcgtgaac aaccatcctc gacgagcctc gtcataaata ccggctgacc aagaccgatc
     2221 cgaggggtca atttcgcatt ggtaaccgta gaccctgccg gtgtcaggcc gctgatgact
     2281 tcggacttgg atccccgagt tgcccggcac atcaaacttg gcttcgacct tcaaaatgaa
     2341 gtcagaaaac gtcttctcgg tgcagaggaa agtgttcgga cccggaccaa ccttgccaac
     2401 aatgcagcca tcttccgtcc aataagtcgc atcaccaccg gccttccgcc aacctgcaat
     2461 ggtcttgcca tcgaacagcg gcacaaaacc ttccgcgtcc ggttccgcct gaggaggcgg
     2521 cagcggaccc aagacctccg ccacatatgg ttcgatggct gctgcccaga tggaatagcc
     2581 tcgggtattg gggtggagca agtcgggcat aatgtctttg gggaggattc cgtcaatcgt
     2641 caaaaacgcc ggaccaatat cgacaaactt gaccatctca ccgtcggcaa actgggcgat
     2701 gatcttattc acctgctcgt tcttctgacg gagcgggtcc tctggcgttt cgccgcgagg
     2761 gaagatcgcc agcaagagaa tctttgtctt tggcaagcgc tcacggagct tttgcacaat
     2821 ggccttgatc ccctcggcag tctcttctgg ttgattgggt gcggcgttat tggttccgat
     2881 catgatcaca gcaagtttcg gctggatgcc gtcaatgttg ccattgtcga ggcgccataa
     2941 aacgtgctgg gtccgatcgc cgccaatgcc aaggttcacc gcattgcgat ggccgtaata
     3001 ggcctcccag atttctttgc ctgctccttc ccagccatgt gtgatcgagt caccaatgaa
     3061 gatcaaatcg acatttccct gttttacccg atcgttcatc gactggtgtc tttgcatcca
     3121 ccactgatct gatcgcggca caggattgac agcatcattg gccaagatgg catgcccgac
     3181 cgttatcaaa agtgtgccaa ccagaagcgt ccccagaaac ccgatccgcc tttttcccat
     3241 agctgcaact ccccaaagca aaaggtttac aaaaacgact cgcaatcaat ccgctcagtt
     3301 taatgagcgg ccgaggtatt gtccacttag gcacaacgga attttgggtc agtggagaag
     3361 ttgcgctggt ctgaagaaaa caattccaac gtaaaaccac tcgttcacca cagttggcct
     3421 atcctgttct ctgacgcatt tggcgcgggg caaatcgcaa cctatggttt gatgaagtgt
     3481 cctgctcgtg cttgttcacg gtcatttccg tgcgaattgc ttgccgtgtg tgacctgcca
     3541 tgagttttga ctttccccgc caattagttt gcattccgcg gtttctcagc cattggaaag
     3601 catccatgcg cccatccagg ggtcggatga gtcatatcgc cggattcttt gggctatgga
     3661 tagccttggt tgggagcttg ctttctgttg cagctttgta taaccggcaa acatgtaaca
     3721 aaccgctcat ttgcttcatc cggaacactc cgccccaaac cgccgagacc aaactgacgt
     3781 caaggaagga ccctgtatac gaaaacccca cgcgggagct tcagacagag gcaccggtcg
     3841 gcgatccgca aaacgcacat gacgattctg ctgcggtgaa agctagcaca cagagtccga
     3901 tattagacaa tcgc
//
LOCUS       DAOSUT010000257         3858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9798_length_3858_cov_9.667105, whole genome shotgun sequence.
ACCESSION   DAOSUT010000257 DAOSUT010000000
VERSION     DAOSUT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9798_length_3858_cov_9.667105"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PLD05_15245"
     CDS             complement(<1..1391)
                     /locus_tag="PLD05_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78841.1"
                     /translation="MLCSRIGIVLCLVVGMGAAWLVDVAQADQDVRPNLLVILADDLG
                     WGDLGCFGSSDLQTPHIDRLAREGMRMLNFYANCPVCSPTRASLLTGYYPDVVGVPGV
                     IRTHSWNSWGYLNPKVELLPNFLAKAGYDTALVGKWHLGLDRPNIPTARGFTYFHGFL
                     GDMMDDYWNHRRHGINYLRENEQEIDPPGHATDLFTEWACQYIRRHHQTQPNRPFFLY
                     LAYNAPHVPIQPPEEALNRYRQRHPDAPEKRSKLAALIEHMDAGIGRVLQTLDELGLT
                     ESTLVVFTSDNGGQLDVGANNGPWRSGKGTMYEGGIRVPMVVRWPNKIQANSQSEAIA
                     LTMDLFPSLLEAAGVEPPTDRDGVSLLPIWLGKAEDLLARELFWVRLEGNKAGFSYAI
                     RRGKYKLIRPAPTKSLAPIEVFDQLYDLEADPYEKQDLAPQQPQLLSEMRRLLENRIK
                     KTIDVPWRDEQGLG"
     gene            complement(1863..2237)
                     /locus_tag="PLD05_15250"
     CDS             complement(1863..2237)
                     /locus_tag="PLD05_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78842.1"
                     /translation="MHPSLLVSVIVGDVLFILGFLAFFFGGAEHPTALIPALFGAVIQ
                     TLAIIAHFFPKTRGPFAKVILIVAFVGLVIGGARPAMVLLGGGQVSWTLALVSQAGLA
                     LGCAVVFCASIVLLLGQAFQKS"
     gene            complement(2273..3421)
                     /gene="carA"
                     /locus_tag="PLD05_15255"
     CDS             complement(2273..3421)
                     /gene="carA"
                     /locus_tag="PLD05_15255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOP78843.1"
                     /translation="MPKPAKLALEDGTVYRGYSFGAEGVVDGEVCFNTSMTGYQEILT
                     DPSDRGQIVVMTYPEIGNYGVNEEDFESWKPHLAGFVVREVSCRVSNRRAQENLDSFL
                     KRFGIVGLYGIDTRALVRRIREHGAMKGVLSTVDLDDASLVAKAKASPGLVGRDLVRE
                     VLPPGPREWAQPLSRWAKLGGQVRERIPPDAPHVVALDFGMKYNIARHLFDSGCRVTV
                     LPGTATAEEVMSFRPDGVFLSNGPGDPEPVQYAQKTIAQLLGKVPIFGICLGHQLLAL
                     ACGARTYKLKFGHRGANQPVMNKETGRVEITCQNHGFAVDPDTLPPYLEVTHWNLNDD
                     TVEGLVHREVPAFSVQYHPEASAGPHDSHYLFGRFRKLIDESRASLAG"
BASE COUNT          859 a         1107 c         1103 g          789 t
ORIGIN      
        1 ccgagtccct gttcatcacg ccacggtacg tcgatggtct tcttgattct attttcgagt
       61 aagcgccgca tctcgctaag aagttggggc tgctgagggg cgaggtcctg tttttcgtag
      121 gggtcggcct ccaaatcgta tagttggtca aacacttcga taggggcaag gcttttcgtg
      181 ggggcagggc ggatcagctt gtatttgccc cgacggatgg cgtagctgaa gccggccttg
      241 ttaccttcca gacgaaccca gaacaattcc cgggcgagga ggtcctcggc cttccctaac
      301 cagattggca gcaggctaac cccatcacgg tctgtcggcg gctcgacacc ggcggcctcc
      361 aggaggctgg gaaacaaatc catcgtgagc gcgatggcct cgctttggga gttggcctgg
      421 attttgttcg gccagcggac gaccatggga acccggattc cgccttcgta catcgtccct
      481 ttaccgctcc gccatgggcc gttgttggcc cccacatcaa gttgaccccc attatcgctg
      541 gtgaaaacca cgagcgtgct ctcagtcagg cccagctcgt caagggtttg caggacacgg
      601 ccgatgccgg cgtccatgtg ctcgatgagg gcggccagtt tcgaccgttt ttccggggca
      661 tcgggatgcc gctggcggta acgatttaac gcctcttcgg gcggctgaat cggcacatgc
      721 ggtgcattgt aggcgagata gaggaaaaag gggcgattgg gctgcgtctg gtgatgcctg
      781 cgaatgtact gacaggccca ttctgtaaac aaatccgtgg cgtgacccgg cggatcgatc
      841 tcctgctcgt tttcacggag gtagttaatc ccgtgacgcc ggtggttcca gtagtcgtcc
      901 atcatgtcgc cgagaaagcc gtggaagtac gtgaaaccgc gggctgtagg gatattgggg
      961 cgatccaatc cgaggtgcca ctttcccacc agggccgtat cgtatccggc cttggccaaa
     1021 aagttcggaa gaagctcgac cttcggattg aggtagcccc aggagttcca agaatgggtg
     1081 cggatcacac cggggacgcc gaccacatcg ggataatacc ccgtaagcaa cgatgcccgc
     1141 gttggcgaac acacggggca gttggcgtag aagttcagca ttcgcatccc ctcccgggcc
     1201 agacgatcaa tatggggtgt ttgcaaatcg gacgaaccaa agcagccgag gtctccccag
     1261 ccaagatcgt ccgcaaggat gacgagcaaa ttggggcgaa cgtcctgatc ggcctgcgcc
     1321 acatcaacaa gccatgcggc ccccatccca acaacaaggc agagcacaat tccaattctt
     1381 gagcacaaca tgggtttcct ctcctgttac cactgaaggt cgatcaccgt ggaattcgtg
     1441 gatattgtaa ccctgttagg gcgacgaggg gaccatcctc ggataatcaa gatgagccgc
     1501 aggtgtgttc tccccaacaa ggcgaagccc gcttgggaac ctcgtaagat agtgggagct
     1561 acctacgacg caagctccac cgacggaaag gagcaccggc ggcggaagga gatcgagtcc
     1621 gtaagccgtg tcccgtcgcg tctgattcga tacaagccgc tagggcaaac tggcgggtca
     1681 tgcgccgtct tattgtgatg gaggattatg aagtaaggac atcgcaagga tatggccgcg
     1741 gcgacattgg cgggtaggtg tcaccgttgt ggccgcccgc cgcttaatct tacacaatgg
     1801 atctcgcaac acagttccgc cggtccaaag cgatgagagg tcaggacctt tgcaaattgg
     1861 gactacgatt tttggaaggc ctgacccagc aaaaggacga tgctggcaca gaagaccacc
     1921 gcgcatccca acgccaatcc tgcctgggaa acaagagcca aggtccaact gacttggccg
     1981 ccccctagga gaaccatggc aggacgtgcg cccccgatca ccagacccac aaaggcaacg
     2041 atgaggataa ccttggcaaa tgggccgcgg gtcttgggaa agaagtgagc gatgatggcc
     2101 aaggtctgaa tgacggctcc gaacaaggcc gggatcaagg ccgttgggtg ttcggcccca
     2161 ccgaaaaaga acgccaaaaa gccgagaata aacaggacgt ccccgacgat cacactcacc
     2221 agcagtgacg gatgcatggt cgcgctcccc ctcgagagat ctcaccggga cgtcagccgg
     2281 ccaacgacgc tcgcgactcg tcgattaact tccgaaaacg gccaaagaga tagtgactat
     2341 cgtggggccc ggctgaggcc tccgggtgat actggacact aaatgcgggg acttctcgat
     2401 gtacgaggcc ctcgacggtg tcgtcgttga gattccagtg ggtgacttcg agataggggg
     2461 gcagggtgtc gggatcaacg gcaaagccat ggttctggca agtgatttct acccgcccgg
     2521 tctctttatt catcacgggt tgattcgccc cacgatggcc gaacttcaac ttgtaagtcc
     2581 gagcaccaca tgccaaggcc agcagttggt gtcccagaca gatcccaaag atgggaactt
     2641 tgccaaggag ctgggcaatc gtcttttggg cgtattggac gggttccgga tcgccggggc
     2701 cattggagag gaatacccca tcaggccgga agctcatgac ttcctcggca gtggccgttc
     2761 ccggaaggac cgttacccgg caaccactgt cgaagaggtg ccgggcaatg ttgtatttca
     2821 taccaaaatc gagggccacg acgtggggcg catcgggcgg aatacgttcg cggacttgcc
     2881 cacccagttt cgcccaacga ctcaagggct gtgcccactc ccgcggacca ggtggtaaaa
     2941 cctcccgaac caagtcgcgt cccacaaggc cgggacttgc cttggccttg gctaccaggc
     3001 tggcgtcatc gagatcgacg gtggatagca cgcctttcat cgccccgtgt tcccgaattc
     3061 ggcgaaccaa ggcccgcgtg tcgatcccat aaaggcctac gatgccaaat cgtttgagga
     3121 acgaatctaa attctcctgg gcgcgccgat tgctaacgcg gcagcttact tcgcgaacta
     3181 cgaatccggc caagtggggt ttccagcttt cgaagtcctc ctcgttgacc ccgtaattac
     3241 caatttccgg gtaggtcatg accacaattt ggccacgatc gctgggatcg gtgaggatct
     3301 cctggtagcc ggtcatcgag gtgttgaagc acacttcacc gtcgacaact ccttctgcgc
     3361 cgaacgagta accgcggtaa acggtgccat cttctaaagc aagtttcgct ggttttggca
     3421 tataacgagg tcgtcccgat gcttgacttc tccgaaagac cacacctcaa aatggaaact
     3481 cgcctaaggc aataaacttc tgccgtttcg cccaactttt cgtcacttgg cggggtctgc
     3541 gatggacctc atggacgtaa agactctcat ctaaagtttg cttcgcaaaa acaccgatac
     3601 gatcaaacac ggctgctgcg gtcccgatca cagacgtcgc atagaagcgt caacgtcgcg
     3661 cggccgaccc aaaggttcct gtcccggcga gcaggaacct ttttgttttt tgtttcttgg
     3721 accgatgctg gtcccgatgg ccactctcct ccaaagcccc actgagcgat cggggcaact
     3781 tagtgtatct tatgcatttt tatcttatct gaccaccaca ccctgggtct gagagcctat
     3841 cccaaagttc cctctccc
//
LOCUS       DAOSUT010000258         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_9924_length_3814_cov_10.672785, whole genome shotgun sequence.
ACCESSION   DAOSUT010000258 DAOSUT010000000
VERSION     DAOSUT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_3814_cov_10.672785"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1491)
                     /locus_tag="PLD05_15260"
     CDS             complement(49..1491)
                     /locus_tag="PLD05_15260"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOP78844.1"
                     /translation="MSRNAILRVSRRRFLTIGGAFVSTLAIPTRSWGDGPSALLAGEG
                     LADITPPLGIELGGFHRPPGQERRVKGIRQPACVRALALRDGEMRAIICSLEVAAVGE
                     VFAKRVREKIAEKAGVPSDHVRIAATHTHSMPAFCFMRQWGAIPEDFMRTVEERTVEA
                     ACRAIDDLAPAELLVGRSTAVGGSHNRTSKNFKTEDEFNAAATDEDRWLDRTIHVLLF
                     PRKGKRTLCWYHFSAHAVCYADELAGPDWPGEVAARLREEEGLDPGFLQGHCGDVNPG
                     DGSDWRGEIKQTVSAIYPALKKAIANVAPVNGRPLRSVRAEFRLPYDMALFQSWLKSY
                     RESPDQCKSGQWVDAGFAEDWYRGNVNRDLSQKELPITLSVLRLGDVAMVFHPSELYT
                     CYGLAIRRDSGFPHTLVVGYTDGFIGYLPDPKAYQAGEYAALTVPKILDYPPFQPEAG
                     RMMTAAAVKILYQLRDYPKKSPSPSERGLG"
     gene            1622..3094
                     /locus_tag="PLD05_15265"
     CDS             1622..3094
                     /locus_tag="PLD05_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78845.1"
                     /translation="MKSLIRRRVACVVCLLSSGITLLMMCGNLQGAEQRLPNILLIVS
                     DDQGYNDLGVLRKDLITPHLDRLAREGVRLTNFYMAWPACTPSRGAFLTGRYPQRNGT
                     YDMFRNEAPDYGYLYTPEEYAVTFERIGGMDTREILIPRILEQKGYRCALFGKWDLGS
                     LKRYLPLARGFHDFYGFVNTGIDYFTHERYGVPSMYRNNEPTTADRGTYITELIQREA
                     LRFLRENHDRPFFLYLAFNAPHNASNLDPRIRSAPQAPEKYLRMYPHLKEEYTSVPNH
                     PYWKNAEVPTRQTRELFYLASITAMDEAIGAVLRELDRFQLTENTFVLFFSDNGGAGP
                     ADNSPLRGHKGQLFEGGIRVCALVRYPNRIPAGTVCDEFLSGLEVFPTFAQLADATIP
                     PDLKLDGFDMLPVLQGQKNSPRERMFWQRQDWKAARVGTWKWIQNRGGEFLFDLATDV
                     GETKNLIEELPTKAQELREAFQQWQAEMEAAEPRGPFRDF"
     gene            complement(3116..>3814)
                     /locus_tag="PLD05_15270"
     CDS             complement(3116..>3814)
                     /locus_tag="PLD05_15270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOP78846.1"
                     /translation="AECPTSRITDAQAFAHRDTSGYAHRQKVGFILDRLDVQSGDHVL
                     DLGAGDGWWTEHLAQKVGPQGTVYAAEVTSTLVERLEKRFAETPQVKPYLCPQDKPGL
                     PDASVDSVFLSAVYHHLNKETRIQYWRELASVVRPTGRVAILETYPVIALRGKDHGTQ
                     LSTLVSEAEQGGWVPLEVWYLQGTQHYLAIFGQQAAFLNNPPTAPHSKAKTCLHLPPG
                     LGCDQGSSAAWRIS"
BASE COUNT          854 a         1091 c         1048 g          821 t
ORIGIN      
        1 cttgaatcac tggcggtttt ctcaatcgat tccgacgttc gcttgccctc accccaaccc
       61 tctctcggag ggagaggggg acttcttggg atagtctctt agttgataaa ggatcttgac
      121 cgctgcggcg gtcatcatgc ggccggcctc cggttggaac ggtggataat cgagaatttt
      181 ggggaccgtc aaggcagcat actcacccgc ctggtaggct tttgggtcag gaaggtaacc
      241 gataaaccca tccgtgtagc ccaccacaag tgtatgaggg aatccggaat cgcggcgtat
      301 cgccagcccg tagcacgtat aaagctccga aggatgaaag accattgcca cgtctccgag
      361 acgaagcaca ctgagcgtga tcggtagctc cttctgagac aaatcccgat taacgttccc
      421 ccgataccaa tcttcggcga atcccgcatc gacccactgg ccagacttgc actggtccgg
      481 cgattctcga taggatttca accaactttg aaagagggcc atgtcatacg ggaggcgaaa
      541 ctctgcccgc accgaacgaa gaggccggcc attgaccggc gctacgttgg cgatcgcctt
      601 tttcaaggcc ggataaatgg cgctaacggt ttgcttgatt tctcctcgcc aatcagaacc
      661 atcaccaggg ttcacatcgc cgcaatgacc ctgaagaaaa cccggatcaa gcccttcttc
      721 ttcgcgcaat cgagctgcaa cttcacccgg ccaatcagga cctgccagtt catcagcata
      781 acacacggca tgtgccgaaa aatgatacca acaaagcgtt cgttttcctt ttcggggaaa
      841 gagcaacacg tggatcgtac gatccaacca acggtcttca tccgtcgccg cggcgttaaa
      901 ctcatcttct gtcttaaagt ttttggatgt ccggttatgg ctgccgccga cggctgtgct
      961 tctccccacg agaagttccg cgggggcgag atcgtcgatg gcacgacaag cggcctcaac
     1021 cgtccgctcc tccacagtcc gcatgaagtc ttccggaatg gcgccccatt gcctcatgaa
     1081 acaaaaggcg ggcatagaat gcgtatgcgt cgccgcaatt cgaacgtggt cactcggcac
     1141 gccggccttt tcggcgattt tctctcgcac acgtttagcg aagacctcac cgacggcggc
     1201 tacctccagg gagcagatta tggccctcat ttcaccatcc cgcagggcaa gagcacggac
     1261 acaggccggt tgtcggatac ccttgacacg ccgctcctga cccggtgggc gatggaaacc
     1321 gccgagttcg atcccgagag gcggtgtaat atcggcaagg ccttcgcccg ccagcagggc
     1381 cgaaggacca tcgccccagg atctcgtcgg tatcgccaga gtggagacaa acgccccacc
     1441 gattgtcaga aaccgacgac gcgaaacacg caaaatggcg ttcctcgaca tgaccttttc
     1501 tccgtgggca actacggcag attgccgctg ctattcaaac ggctttattc tagccacaat
     1561 ggtgtaccga gggaacgaag tagggagcgc agtatttctg gtgaagtagc gggaccaaac
     1621 catgaaaagt ctgatccgtc gtcgagtggc gtgcgtggtg tgcctgctat ctagcgggat
     1681 cactctcttg atgatgtgcg gtaatctcca gggggccgaa caacggttgc caaatatcct
     1741 gcttatcgtc agcgatgacc aaggctataa cgatttgggt gtcctgcgga aagacctcat
     1801 aactccgcac ctggatcgtt tagcccggga aggtgtgcgt ttgacgaact tctacatggc
     1861 ctggccggcg tgcacgccga gccgtggggc ctttctcaca ggacgttatc cccagcgaaa
     1921 cggcacctac gacatgtttc gcaacgaggc ccctgattac ggctatcttt ataccccgga
     1981 agaatacgcc gtgacctttg agcggatcgg cggaatggat acgcgagaaa tccttatccc
     2041 acgaatattg gaacagaagg gctaccgctg cgccctgttt ggcaaatggg acctcggctc
     2101 cttgaagcgg tatttgcctc tggcccgagg gttccatgat ttctacggct ttgtcaatac
     2161 gggtatcgac tacttcacgc atgagcggta cggtgtccct tcgatgtatc gcaacaatga
     2221 acccaccacc gcggaccgtg gcacttacat aacagagtta atccaacgcg aagccctgcg
     2281 gttcctcaga gagaatcatg atcgtccctt tttcctttac ctcgcattca acgcccccca
     2341 taacgcaagc aatctcgatc cgcggatccg atcagccccc caggcccctg aaaagtacct
     2401 gcggatgtat ccccatctga aggaagagta cacgtccgtc cctaatcatc cttattggaa
     2461 aaacgcagag gtaccgaccc ggcagacacg ggagcttttt tatcttgcct cgatcacggc
     2521 gatggacgag gcgattggtg ccgtactgcg cgagctggat cgctttcagt tgacggaaaa
     2581 tacgtttgtg ctctttttct cggacaatgg tggtgccgga ccggcggata actctccgct
     2641 gcggggtcac aaaggccaac ttttcgaagg gggaattcgt gtctgtgccc tggtccggta
     2701 ccccaacaga atccccgccg ggacggtgtg cgacgaattc ctcagtggtc tcgaggtgtt
     2761 ccccaccttt gctcagcttg ccgacgcgac catcccgcca gatctgaaac tcgacggttt
     2821 tgatatgctt ccggtcctcc aggggcagaa gaattcgcca agagagagga tgttctggca
     2881 gcgacaagat tggaaggcgg cgcgtgtggg cacctggaag tggattcaaa accgcggcgg
     2941 tgaattcctt ttcgacctgg cgacggatgt aggagagacg aagaatctga tcgaagagtt
     3001 gccgacaaag gcccaggaat tgagagaggc cttccaacag tggcaagcgg agatggaggc
     3061 cgccgaacct agagggcctt tccgtgactt ttaagctgtg cccgtgaatc tgttattaac
     3121 ttatccgcca tgcagcagac gagccttgat cgcagcccaa gccgggcggg agatgtaaac
     3181 aagttttagc cttactgtgc ggggcggtcg gcgggttgtt caaaaaagcc gcttgttgtc
     3241 caaagatggc aaggtagtgt tgcgttcctt gcaagtacca aacctctagg ggtacccaac
     3301 caccttgctc ggcctcgctg accagcgtgc tgagttgtgt cccatgatcc ttgccgcgca
     3361 aagcgatgac aggataagtt tcaagaattg ccacccgtcc cgttggccgc acgacgcttg
     3421 ccaattctcg ccagtattgg atccgcgttt ccttgttgag atggtgatag acagcggaca
     3481 agaataccga atccacgctg gcgtccggca gacctggttt atcctgagga caaagatacg
     3541 gcttgacctg aggggtctcg gcaaaccgtt tctctaagcg ttccaccaaa gtagaggtga
     3601 cttcggcagc gtaaacggtg ccctgcgggc caaccttttg ggcaaggtgt tcggtccacc
     3661 acccgtcgcc ggcacctagg tccaagacgt gatcgcccga ctgaacgtcc aggcggtcta
     3721 ggataaaacc aaccttctgt cggtgggcgt aaccgctggt gtctcgatgg gcgaacgcct
     3781 gagcatcggt gatccggctg gtaggacatt cggc
//
LOCUS       DAOSUT010000259         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10050_length_3777_cov_7.201236, whole genome shotgun sequence.
ACCESSION   DAOSUT010000259 DAOSUT010000000
VERSION     DAOSUT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10050_length_3777_cov_7.201236"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1051
                     /locus_tag="PLD05_15275"
     CDS             263..1051
                     /locus_tag="PLD05_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOP78847.1"
                     /translation="MTKISEVVHFLGTLCPPILAEDWDNTGLLLGDPELPVEAVMTCL
                     TLTRKTCHEAIDKMANLVVCHHPMPFRPVRCITTVSQSGRILWELANHGIAVYSPHTA
                     FDSCESGINAMLARMLRLHDVRPLRPLPQDPLVGAGRMGCLEQPVSLLEFAHRVKEQL
                     GVKAVQLVGRPEASVSRVAVACGAAGELLDDARACGCDVIVLGEARYHTCLEAEFWGI
                     GMVLVGHYASERRGVEKLAEILRRHFPHLKVWASEQESDPMCWV"
     gene            1190..1948
                     /locus_tag="PLD05_15280"
     CDS             1190..1948
                     /locus_tag="PLD05_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOP78848.1"
                     /translation="MACTQHRDAVALVTGAGKRRVGSFIVRMLADRGYKVAIHYRSSQ
                     QEAESLKHELVSQGKQAEIFQADMADTASIDQMFERVLGHFGRLDVLVTAAAIWEPTR
                     FETLTPESVMEHFRVNALGAFWCCRRAGLTMVGQPEGGVIITIGDWAIERPYLNYASY
                     FLSKGCIPTMTRVLAIELSHRNPNIRVNAIMPGPVMMPSHLPEEEKVEAIRSTLLQKE
                     GKPEHIVSAVEFLIDNDYVTGVCLPVDGGRTIGG"
     gene            complement(2040..2969)
                     /locus_tag="PLD05_15285"
     CDS             complement(2040..2969)
                     /locus_tag="PLD05_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78849.1"
                     /translation="MNAVDSETPASSPLGAILLVAETRAQGSLRERLRERRETRSLAR
                     NREKDPLVPQEPNDWQVGRRITRERVQTWIEQLRDRIRAEIGDSDGARDLEIPRVPGG
                     RTDSALPSTVAPPGNTWPPRPLVPTDQQLSAGVKDSSSSQLQQPEVIGPELVPPESPS
                     APPSAMKNAHPDSAVPFSTIESPPPTSLPGTTEKPVEDNRNQDSTGLDPGGEGVTSQP
                     GATAASKPTGLSGLFSNVAQMFGTATSPRVEKPSKDESQENQENQAGSNQTADPQNVP
                     ASSKPRSTTGKTLAEIRKQILLKQSSKSAAPRQ"
     gene            complement(3313..>3777)
                     /locus_tag="PLD05_15290"
     CDS             complement(3313..>3777)
                     /locus_tag="PLD05_15290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOP78850.1"
                     /translation="FPKEPEAAKARGALRRLTSEGQPFELKGSDLQGGTVDVAAYRGK
                     ILIVHFWTSSCEPCKTDMAIMKDLVTRYGGKLAIVGVNLDTRKEDLQAYLVEARLPWP
                     QIHEPGGLESRPAIEYGIVTVPTTLLIDAQGRVVKRQVTGSELESEIRRLLQ"
BASE COUNT          778 a          979 c         1112 g          908 t
ORIGIN      
        1 ttccacccta ttgtattatt tgacaaatcg agcgagaatc aagtgcctca ttcgggagga
       61 tgttttgcct ggtaggtgat gtcgtcttcg ggttgaggaa aaggaagtga cccgtacgtc
      121 aaaagggcgc atttccaata atggtattac ggcggagatg gcaaaaatgt gtcgggctca
      181 aagtcgagaa tagatggtaa aggtctgtcg gggacgcagg ttaagacacc tgaggtgtga
      241 tttacgcaag ggctatcagg tcatgacaaa aatctctgag gttgtccatt tcctcgggac
      301 attatgcccc ccgatattgg ccgaagactg ggacaacacc ggacttctgc tgggggatcc
      361 cgagcttccc gttgaggccg tcatgacatg tttgacattg acccgcaaaa catgccacga
      421 agctattgat aaaatggcga acctggtggt gtgtcatcat ccgatgccgt ttcggccggt
      481 gcgttgcata acaacagtgt cgcaatctgg gcgtattctg tgggaattgg ccaatcatgg
      541 catcgccgtt tacagtcccc atacagcttt cgattcatgt gaaagtggta ttaacgcgat
      601 gcttgcccgg atgctccgtc ttcatgacgt gcgaccgctt cgaccgcttc cgcaagatcc
      661 acttgttggt gcgggacgga tgggctgttt ggaacaaccg gtttcgttgc tcgagtttgc
      721 acatcgggtg aaagagcaac tgggggtgaa agcggtgcaa ctggtcggtc gcccggaggc
      781 ctctgtttcg cgggttgctg tggcgtgcgg tgccgccggc gagttgctgg acgacgctcg
      841 ggcgtgcggt tgcgacgtga ttgtgctcgg cgaggcaaga tatcatacct gcctagaagc
      901 ggagttctgg gggatcggta tggtcttggt cggccactat gctagcgaaa ggcgtggcgt
      961 agagaaactg gctgagattc tccgccggca tttcccgcac ctcaaggttt gggcctccga
     1021 gcaagagtcc gatccgatgt gctgggttta agtgctaact gatttataaa attttcttgc
     1081 cactcttaag gcagtggtaa gaaggtactc actgagaaga atccgcaatt aggaataaaa
     1141 ttaatcacga caccccgcgt cggctaggaa tagctaggag tggtacggta tggcttgtac
     1201 tcaacatcgt gatgcagtcg ccctggtaac cggcgctgga aagcgacgcg ttggcagctt
     1261 cattgtgagg atgctcgctg accggggata caaggttgct attcattacc gcagctcgca
     1321 gcaggaggca gaatcgctca agcatgagtt ggtatcccaa ggcaagcagg ccgaaatctt
     1381 tcaggccgac atggcggata ccgcctccat tgatcagatg ttcgagcggg tacttgggca
     1441 ttttgggcgg ctcgatgtcc tggttacggc agcagcgatc tgggagccga cgcgttttga
     1501 gacgctaacg cccgaaagcg tgatggagca ttttcgagtc aacgcattgg gggcgttttg
     1561 gtgttgtcgc cgtgcgggtt tgaccatggt cggccagccc gaaggcggtg ttatcatcac
     1621 aattggcgac tgggctatcg agaggccgta cctgaactac gcaagctatt ttctctcgaa
     1681 gggttgtatc cccaccatga cgcgggtctt agcgattgag cttagtcacc ggaatcccaa
     1741 cattcgcgtc aacgctatta tgccggggcc cgtcatgatg ccaagccatt tgccagagga
     1801 agagaaagtc gaggccattc gcagcacgct gctgcaaaaa gaaggaaaac cggagcacat
     1861 cgtttcggca gtggagttcc ttattgacaa cgattacgtc accggcgttt gtcttcccgt
     1921 cgatggaggc cggaccattg gtggctgaga caaatgctgc tgccggacga aaacaaagac
     1981 acagtgtttg cccccgtgga gcgactgtgg taccttgtgt cacgttgatc acgggccact
     2041 cattggcggg gggcagcaga ctttgaactt tgtttcaaga gtatctgctt tcggatttcg
     2101 gcaagcgttt ttcccgtggt ggaccgaggt tttgagctcg cgggcacgtt ctgagggtcg
     2161 gccgtttgat tagagccggc ttgattctct tgattctcct gggattcgtc tttgctgggc
     2221 ttttcaaccc tgggcgaggt ggcggttcca aacatctgtg ccacattgga gaacagcccg
     2281 ctgagaccag ttggtttgct cgcagcagtc gcaccgggtt gcgaagtgac cccctcacca
     2341 ccgggatcta agccggtgct gtcctggttg cgattgtctt ccaccggctt ttcggtagtt
     2401 cccggcaagc ttgttggcgg aggggactcg attgtgctaa acggaacggc agaatccggg
     2461 tgcgcatttt tcatcgcgga gggtggggcg gagggcgatt ccggtgggac gagctcaggt
     2521 ccgattactt ccggttgctg cagttgacta ctggacgagt cctttacacc tgccgacagt
     2581 tgctgatctg tcggcacaag cggacgaggc ggccaggtgt tcccgggcgg tgccacggta
     2641 cttgggagtg ccgaatcggt tctcccacca gggacccgtg ggatttccag atcgcgggca
     2701 ccgtcggaat cgccgatttc tgcccgaatt ctgtcgcgca actgttcaat ccatgtttga
     2761 actcgctcgc gggttatccg gcggccgacc tgccaatcgt tcggttcttg agggaccaat
     2821 gggtccttct cgcggttcct tgcaagagaa cgtgtttccc gtcgttcccg gagacgctca
     2881 cggagactac cttgcgccct tgtttcagcg accagaagaa tcgcacccag cggggaagac
     2941 gcaggtgttt cgctgtccac ggcgttcacc gctatccttc tagaatcggc atgcctttct
     3001 cgagactccg ccacggggat gggcaaaatc caaactgatg ttacccacag aagtagtatt
     3061 aggcgtgacc gaagaacacc cgaactgtgg cgtaatacgt tactcataaa acccatggtc
     3121 tcatctccgc cccgggatgt ttcgctcacc aacagcacga caatagtctt tccgcccgcg
     3181 cggtgatcgt actctgcgaa tctaccgcat ttcaacgcac aaagacaggc tgtggcgagc
     3241 ctgtctttgt agtccgtaac aatatgcacg gttgatacgc agtcgtgcgg tcattctgga
     3301 ccatcgttca agttattgga gcaaacgtcg aatctcggat tcaagctctg atcccgtcac
     3361 ctgacgtttg accacgcgtc cctgggcatc aatcagcaaa gtggtcggca cggtgacgat
     3421 tccgtattcg attgccggac gactttccag tcctcccggt tcgtgaatct gtggccaagg
     3481 aagacgtgct tcaacaagat aggcctgcaa atcttccttc cgggtgtcga gattgacccc
     3541 gacaatggcc agcttcccgc catatcgcgt gacgaggtct ttcatgattg ccatgtcggt
     3601 cttgcacggt tcgcacgaac tggtccagaa atggacaatg aggatttttc cgcggtacgc
     3661 tgccacatcg actgttcccc cttggagatc gctgcctttc agttcgaaag gctgaccttc
     3721 gctcgtcaac cgccggaggg caccgcgggc cttggccgct tcgggttcct tgggaaa
//
LOCUS       DAOSUT010000260         3771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10078_length_3771_cov_11.622443, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000260 DAOSUT010000000
VERSION     DAOSUT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10078_length_3771_cov_11.622443"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..2274
                     /locus_tag="PLD05_15295"
     CDS             481..2274
                     /locus_tag="PLD05_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOP78851.1"
                     /translation="MELPKRPKDDRFTVPGKYRELEQSVRQKAASHDIPALIVYAFDH
                     RTSLGPFVMVQNWMIPGAPRALASAMYAAGFHKLRVVMQQWTPNVRPSQARIDGQVPQ
                     LLLISSMQIHSARAYELIRDAWTLGDERPLIIAGGAKAIYEPWDFFGLSEDGSVGADV
                     VCTGEEYVLMEFLDRLLDYKLPGEHIRAAFERAREQGVFNDIPGLVFRPDPPRGHPEY
                     LVDTGVQRLVQDLDEFPLPFDALSLFEPPHKRTTLSPEPIPVDQLGKYAKVLAVVTTH
                     GCKFRCPYCPIPAYNQESFRYRSPERIVEEFAGIHSRTGIRYFFGTDDNFFNNRQTVE
                     EVFSAMAKATVNNKPFRDAIIFATEGTEFDVHKNIDLIPLGREAGLRSIWFGIEDLTA
                     DLVKKGQSADKTRVVFRELIKHGIAPMPMMMHHDNQPLWSWGSLRGLINQVGFLRKVG
                     AITCQITLLTPSVGSKGFEKPFEDGIVLKKVAGKPVEDYHYDGNRAIATAHPSPLRRQ
                     LNVLAGYLAFYNPLNLLRALPRLDPLWKERIFTQIQGMIGVAKSIWNAREWFYDLAVG
                     PIEYHTAPPQPKYPLVTPVVRIQESRVPAMG"
     gene            2284..3000
                     /locus_tag="PLD05_15300"
     CDS             2284..3000
                     /locus_tag="PLD05_15300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOP78852.1"
                     /translation="MRRDGLPGYALAVLLVVLMTVPTAAALGYFVLLESAPSLVQQSA
                     YVAGKVVMTLPAIWFFFVEGGRLRWSPPRAHDIRDGIAFGLLVLAGMLGIYFVFLKPA
                     GILAFAAPAIQQKVMGFGLVTPARYVAFAGMISLLHSALEEYYWRWFVFGRLRDPIGP
                     LWAAIIASAAFTGHHVVILGRYFGWLSAGQVLFSLAVFFGGVIWCAMYHRSRSLYGPW
                     ISHALIDAAIFVVGYDLALS"
     gene            complement(3025..>3771)
                     /locus_tag="PLD05_15305"
     CDS             complement(3025..>3771)
                     /locus_tag="PLD05_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOP78853.1"
                     /translation="EIPLAPNPFPPKDCPQIALTDWGELRAYTGIPAKGPLSEEMARD
                     LVHGYYAATSYVDAQIGKLLAEVERLGLKDETIVVLWGDHGWHLGDHGLWCKHTNFEN
                     ATRSLLIFRVPGQKTAGQKTDALVEFVDIYPTLCELCNLPIPEGLEGTSLVPLFEDPN
                     RPWKKAAFSQYPRSGVMGYSMRTDRYRYTEWRPRKGGEPVAVELYDHAEDPQENVNLA
                     NRPEYQNLVNELKKQLEAGWQGALPPKPAE"
BASE COUNT          734 a         1034 c         1108 g          895 t
ORIGIN      
        1 tccctgccct caccccaacc ctctcccgga gggagagggg gactttttgg gatagactct
       61 gaggccataa tttctcgctt aaaaagtgtg aactaggctt tggttctgca ttttcgattt
      121 tcgccatccg agaatatcta taatcacccg gccggtcgtc tttgttggac agtgaatcgg
      181 cttatggggc gagcgtgaca aagctgacaa cggtggacac caaaggcgac aaagttactt
      241 tctctgccaa gtgacggaag aaagccctat aaccattttg ggggaaatga cgtccggacc
      301 tttgggtcga tgtgcttttt ttgtaccttc taaggctagt aattggccgg gtagagcgta
      361 taatttgggt caacgcatcc gcaatgggat tgagccaccc tgcccgtgcg gagcggtggg
      421 ccattcctga atggtctgat ttgaaacttt gcgtgtggct gcgatttgga ggttattgct
      481 gtggaattgc ccaagagacc taaggacgat cgattcaccg tccctggaaa atatcgagag
      541 ttagaacaaa gtgttcgaca aaaagccgct tctcatgaca ttcctgcgct cattgtttac
      601 gcctttgatc accggacgag cctcgggccg ttcgtgatgg ttcagaattg gatgatccct
      661 ggcgcgccgc gggcgttggc ttctgccatg tatgcggcgg gattccacaa attgcgggtt
      721 gtcatgcagc agtggacgcc taacgtccgt cccagccagg cccgcatcga cggccaagtc
      781 ccgcaactgc tgctgatttc cagcatgcaa attcacagcg cgcgggcgta tgagctcatc
      841 cgcgacgctt ggacactcgg agacgaacga cctctgatta tcgccggggg ggccaaggcc
      901 atttacgagc cgtgggattt cttcggccta tccgaagacg gttccgtagg ggcagacgtg
      961 gtctgcacgg gcgaggagta cgtcttgatg gaattcctcg accgactttt ggactacaag
     1021 ctcccgggcg agcacatccg ggctgctttt gagcgggcac gcgagcaagg cgttttcaac
     1081 gacatcccgg gattggtctt ccggcccgac ccgccgcgtg ggcatccaga gtacctcgtc
     1141 gatacaggcg tccaacgact ggtccaagat ttggatgagt tcccgctgcc cttcgatgcc
     1201 ttgtcgctct ttgaaccgcc gcacaagcga acaacgctgt cgcccgaacc gatccccgtg
     1261 gatcaactgg gaaaatacgc caaagtgttg gccgtcgtga ccacgcacgg ctgcaagttc
     1321 cgttgtccct attgtcccat tccggcatac aatcaggagt cgttccgtta tcggagcccc
     1381 gaacggatcg tggaggagtt tgcgggcatt cattcgcgga cggggattcg ctatttcttc
     1441 gggacggatg acaacttctt caacaatcgc cagacggtcg aggaagtctt ttcggccatg
     1501 gcgaaggcca ccgtcaacaa caagccgttc cgtgatgcga tcatcttcgc caccgaggga
     1561 acagaattcg atgtccacaa gaacattgac ctgattccgt tgggacggga ggctgggttg
     1621 cggagcatct ggtttggcat cgaggacctg acggccgatc tcgtgaagaa aggacaatcg
     1681 gccgataaga ccagggtcgt gttccgcgaa ctcatcaagc atggcatcgc ccccatgccg
     1741 atgatgatgc accacgacaa ccaaccattg tggagttggg gtagcctgcg gggtctcatc
     1801 aatcaggtgg gctttctccg taaagtgggt gcaatcacct gccagatcac tttgttgacg
     1861 ccttctgtgg gaagcaaggg ctttgaaaag ccgtttgaag acgggatcgt gttgaagaag
     1921 gtggccggca aaccggtgga agattatcac tacgacggca accgggcgat tgccacggca
     1981 catcctagcc ccttgcgacg gcagttgaac gtccttgcag gctacctggc attttataac
     2041 cccttgaatc tgctgcgtgc cttgccgagg ctcgatcccc tctggaagga gcggattttc
     2101 actcagattc aggggatgat cggggtggcc aaatcgatct ggaatgcgcg ggagtggttc
     2161 tacgatctag ccgtgggacc gattgagtat cacacggcac cgccgcagcc caaataccca
     2221 cttgtcaccc ctgtggtgag aatacaagaa agccgtgttc ctgccatggg atgagcttgg
     2281 cccgtgcgcc gcgatggctt gcctggttat gccctggcgg tcctgcttgt ggtgctgatg
     2341 acggtcccga ccgccgcagc gctagggtat tttgtcctgc tagaatccgc cccatcgctg
     2401 gttcagcaaa gcgcctacgt cgccggcaaa gttgtgatga ccttgccggc gatctggttc
     2461 ttttttgttg aaggagggcg attacgatgg agccctccgc gggcgcatga catccgagac
     2521 gggattgcct tcgggttgct cgtcctggcc ggaatgttag ggatttactt cgtttttctt
     2581 aaaccggcgg gcatcctcgc ttttgcggca ccggccattc agcaaaaagt gatgggcttt
     2641 gggcttgtga caccggctcg gtatgtggca ttcgccggga tgatttccct gctgcattcc
     2701 gccctggagg aatactactg gcgctggttc gtctttggca gactgcggga cccaatcgga
     2761 ccgctttggg cagccatcat cgcttcggcg gccttcaccg gccatcacgt cgtcattctt
     2821 ggacgctact tcggctggct gagtgctggt caggtcctct tctcgcttgc cgtctttttt
     2881 ggcggggtca tctggtgtgc catgtatcac cggtcacgat ccctttacgg accgtggatc
     2941 agccacgctt tgattgacgc ggcgatcttc gtggtcggtt acgacttggc cctaagctga
     3001 cgcccctgcc gggatctttg gtcatcattc cgccggtttt ggcgggaggg cgccttgcca
     3061 gcccgcttcc aactgctttt tcagctcgtt gaccaggttc tgatactcgg gacggttggc
     3121 gagattgacg ttttcctgag ggtcctcggc gtggtcatac agttcgaccg ccaccggctc
     3181 gccacctttt cgggggcgcc attcggtgta acgatagcga tccgttcgca tggagtagcc
     3241 cattacgcca gagcggggat attgactgaa ggccgccttt ttccagggcc gattcgggtc
     3301 ctcgaaaaga ggaaccaaac ttgtcccctc caatccttcg ggaatgggta agttgcataa
     3361 ttcgcacaag gtgggataaa tatcaacgaa ttccaccagg gcatcggtct tttgcccggc
     3421 ggttttctgg ccaggcacgc ggaagatgag cagcgaccgc gtggcgtttt cgaagttcgt
     3481 gtgtttgcac cacaagccgt ggtcacccag gtgccagccg tgatcgcccc agagcacgac
     3541 gatggtctcg tctttgaggc cgagccgttc cacctcggcg aggagcttac cgatttgggc
     3601 atcgacgtaa cttgtggccg cgtaataacc gtggaccagg tcgcgagcca tttcctcgga
     3661 gagaggacct tttgccggga ttcccgtata tgcccgtaat tcaccccagt ctgtcaaggc
     3721 aatctggggg caatctttgg gcggaaaggg attcggtgcc aaggggattt c
//
LOCUS       DAOSUT010000261         3771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10079_length_3771_cov_11.012917, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000261 DAOSUT010000000
VERSION     DAOSUT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_3771_cov_11.012917"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2760
                     /locus_tag="PLD05_15310"
     CDS             <1..2760
                     /locus_tag="PLD05_15310"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOP78854.1"
                     /translation="KYLVEMSAAEKTRTLARFNRELRDYFASYVRPEFLKRLGPDEFL
                     PEVLSTSYVARHVFEGVNEERDSYVRFTARNPRNPLNRPTPGEAWLLEYFEKNPSADS
                     WSGRLKFFEGGQPYYVVAYARRFEESCLRCHGRPEDAPSGLIKRYGDQAGFHYKVGDV
                     SLELMAVPMDRAMSQASQQVIYYLLGAIGVCGLFIGGVIGLVVLHRVQERRLQQELHK
                     GREFLAATLRSIGDAVISCDCHGRIVSLNSVAEKLTGWSNEEALGVSVEDLFSIFDSR
                     TGERLPNLVHQVISCGQALELAGHIKLMTRDGRSYDIAESCAPIRDLLGQVIGAVLVF
                     RDVNEQYRQQDKLRESQDQLRAITDSVQDAIIMIDPDGKITFWNPAAEKIFGYTAREV
                     MGLSVHEFLCLDEDRQRFLRSYPQFVENGEGPAVGRTLELRARRKDGQIITVELSLGA
                     VKLQSGWHAVAVVRDITQRKRNEEELQRYALALEAANKSLEQFGFQAEAATRAKSAFL
                     ANMSHEIRTPMTAILGYTELLLEELQKSGASREHLEALETIQRNGNYLLKLINDILDL
                     SKIEAGQLKVEPQSLNLPELLEDVIKLMRVRAEAKALPLVLEFAGPIPKLIRSDPVRL
                     RQILINLVGNAIKFTEEGEVRLRVRLAGEQEQSQKLCIDVIDTGIGIERDKLEHIFEP
                     FSQGDSSTTRKYGGTGLGLTISRRLAQCLGGDITVVSAPGKGSVFTVTIDPGPIDECV
                     EPNQSAMIKSGAASVEAKENPSAIRLDARILLAEDAPDNQRLISFILKKAGATVTVVK
                     DGRAAVEAAMKAWENGEPFELILMDMQMPEMDGYQATRYLRDRGYSGKIVALTAHALP
                     EDVTKALDAGCDAYLTKPIRRETFCKSLSKILAQSAPDVGPSDSNMAAGAPDVEVDPG
                     PMPVG"
     gene            complement(2998..>3771)
                     /locus_tag="PLD05_15315"
     CDS             complement(2998..>3771)
                     /locus_tag="PLD05_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOP78855.1"
                     /translation="YGMTNDRRLGPLLDQALKVVQQSQCGDGGWDYVPKRQSQGHDLS
                     LVVMQALALRSAVDSGFEISPQVVQAAIECVREHYTPKNCSRDAPEEEQQRHPGQFTY
                     SKGGGNATIAMAAAGVVCLQEFGQYDDWRIGKNMEVIRAAILEATRDGRPRRERRAPF
                     DPYTLYYVAQALYQVGGDAWQECYPPLRDAVVASQIREPNQPSRDGMWTAQGHVGGRP
                     GELYMTAVCCFVLAIPNRYLPILQEGRIETLQKQFANAP"
BASE COUNT          842 a          966 c         1105 g          858 t
ORIGIN      
        1 aaatatcttg tggaaatgtc cgccgccgaa aaaaccagaa cgttagcgcg ctttaatagg
       61 gaattgcgcg actactttgc ctcctacgtt cgtcccgaat tcctgaaacg gcttggacca
      121 gatgagtttc ttcccgaagt tttatctacc tcctatgttg cgcgtcatgt ttttgaaggg
      181 gtgaacgaag agcgagatag ctatgttaga ttcactgcaa ggaatccccg caatccgttg
      241 aatcggccga cccccggtga ggcgtggctg ctggagtatt ttgagaaaaa tccaagtgct
      301 gattcatggt cgggaagact taagtttttt gagggcggac aaccgtatta tgtcgtcgcc
      361 tatgcgcgac ggtttgagga aagctgcctc cggtgccatg ggcggccgga ggatgctcct
      421 tcggggctca tcaagcgata cggtgaccag gctggctttc attacaaagt gggcgatgtc
      481 tctttggagt tgatggctgt gcccatggat cgggccatgt ctcaagcttc gcaacaagtc
      541 atctattatt tgcttggcgc catcggagta tgcgggctat ttattggagg tgtcattggt
      601 ctagtcgttt tgcatagggt ccaggagaga cggcttcaac aggaactcca taaaggtcgt
      661 gagtttttgg ctgctacgct acgatccatt ggagacgctg tcatctcatg cgactgccac
      721 gggcgaatcg tgtcgctcaa ttctgttgcg gaaaaactta caggttggtc gaacgaagag
      781 gccctgggag tatccgtcga agatctcttc tccattttcg acagtcgtac gggcgagcgg
      841 cttccgaatc ttgttcatca ggtgattagc tgtggccaag cattggaatt ggctggtcat
      901 atcaagctga tgacacgaga tgggcgttcg tatgatattg cggaaagttg tgctccgatc
      961 cgtgaccttc tgggccaggt tatcggggcc gttctagtgt tccgagatgt caacgagcag
     1021 taccggcagc aagataagct ccgcgagagc caggaccaat tgcgggcgat cacggattct
     1081 gttcaagatg cgatcattat gattgacccg gatggtaaga tcaccttctg gaatccggcg
     1141 gcagaaaaga ttttcgggta cacggcgaga gaggtcatgg gactcagcgt ccatgaattt
     1201 ctctgcttgg atgaggatcg ccaaagattc ttgcgtagtt atccacagtt tgtagaaaac
     1261 ggggagggcc cggccgtcgg tcggacattg gaactgcgag cgcgtcgcaa ggatggacag
     1321 attatcacgg tggagctgtc actaggggca gtgaaacttc aaagtggttg gcatgccgtg
     1381 gcagttgttc gtgatattac ccagcgaaag cggaacgaag aagaattgca gcgatacgct
     1441 ttggcactgg aggcagccaa taagagcctg gagcaatttg ggtttcaggc cgaggccgcc
     1501 acccgtgcca aaagtgcatt tctcgccaat atgagtcacg agatccggac gccaatgacg
     1561 gccattttgg gctacacgga gctccttctc gaggaacttc aaaaatcggg agcatcgcgc
     1621 gagcacttgg aggccttgga gacgatccag cgaaatggga attatctcct gaagcttatc
     1681 aacgatatcc tggatttgtc aaaaatcgag gcaggacaac tgaaggttga gcctcagtcg
     1741 ttgaatttgc ctgagctgtt agaggacgtc attaagctca tgcgggtgcg tgctgaggcc
     1801 aaagccctgc cgctcgttct cgaattcgcg gggccgattc ccaagttgat ccgcagtgac
     1861 cctgtccgac tgcggcaaat attgatcaat ttggtgggaa atgccatcaa atttaccgaa
     1921 gaaggggaag ttcgattgcg ggtacggttg gccggcgagc aggaacaatc gcagaaactg
     1981 tgcatcgacg tgattgatac agggataggt atcgagcgcg acaagctgga gcatatcttc
     2041 gaaccgttct cccaaggtga ttcgtcaaca acgcgcaaat atggtggcac ggggttaggg
     2101 ctgaccatta gccgtcgtct cgcccagtgc ttgggcggcg acatcactgt ggtgagcgca
     2161 cccggcaagg ggagtgtgtt caccgtgacc attgaccccg gtcctattga cgaatgcgtt
     2221 gagccgaacc aatcggcaat gataaagtcc ggtgctgcat cggtggaagc aaaagagaac
     2281 ccctcagcga tacggttaga tgcgcggatc ttgctggcgg aggatgcccc ggacaatcag
     2341 cgtttgatct catttatcct gaaaaaggcc ggagcgacgg tcaccgtggt caaagatggc
     2401 cgggcggcgg ttgaggctgc gatgaaggca tgggaaaatg gagagccgtt tgagctgatc
     2461 ctaatggata tgcagatgcc cgaaatggat ggctatcaag cgacccgata tctcagggac
     2521 cgcggttact cgggaaaaat cgtggctctg acagcacatg cccttcctga ggacgtgaca
     2581 aaggcccttg acgcggggtg cgatgcatac ctgacaaagc cgatccgtcg cgagaccttc
     2641 tgtaagagct tgagcaagat ccttgcgcag tctgcccctg atgtcggacc ttccgactcg
     2701 aacatggctg ctggagctcc cgacgtcgag gttgaccctg ggcccatgcc cgttggctga
     2761 gatttctctt cgtcatcccg gtttgcgctg tcaatgtgca caatccgact taaggcctac
     2821 cccaaaaggc ttcccctctc cctccgggag aggtttgggg tgagggcaag cgatcgtcgg
     2881 aatcggttga gaaaaccggc agcgactcga gccaccctca cccccgcccc ctctcccgcc
     2941 aaacgggcga ggggagattg attgtgggct agacttttag gtgaaagcga tttcccttca
     3001 aggggcgttg gcaaactgct tttgcagtgt ttcgattcta ccctcctgaa ggataggcaa
     3061 ataacggttg ggaatggcga gcacgaaaca acagacagcg gtcatataga gttcgccggg
     3121 gcgaccgccg acgtggcctt gggcggtcca catgccgtcc cgcgacggtt ggttcggctc
     3181 gcgaatttga ctggccacaa ccgcgtcacg caagggagga tagcattctt gccacgcatc
     3241 gcccccaacc tgatacaatg cctgtgccac gtagtaaagt gtgtaaggat caaacggagc
     3301 acggcgttca cgtcggggac gaccatcccg ggtcgcttca aggatagctg cgcggattac
     3361 ctccatgttc ttgccaatcc gccagtcgtc gtattgtccg aactcttgga gacacacaac
     3421 gcccgccgca gccatggcga tggtggcatt accccctccc ttgctgtagg tgaactggcc
     3481 cgggtgccgc tgttgctcct cttctggtgc atcacggctg caattctttg gggtgtaatg
     3541 ctcccggaca cattcaatgg cagcctgaac gacctgggga gagatttcaa aaccgctatc
     3601 cacggcgctc ctcaatgcca gcgcctgcat cacgaccagg ctcaggtcat gtccctggga
     3661 ctggcgtttg ggtacgtaat cccagccacc gtctccgcac tgactttgct gtaccacctt
     3721 gagcgcctgg tcgagcagtg gccccaaccg tcggtcattg gtcatcccat a
//
LOCUS       DAOSUT010000262         3724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10228_length_3724_cov_9.829654, whole genome shotgun sequence.
ACCESSION   DAOSUT010000262 DAOSUT010000000
VERSION     DAOSUT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10228_length_3724_cov_9.829654"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLD05_15320"
     CDS             complement(<1..1092)
                     /locus_tag="PLD05_15320"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOP78856.1"
                     /translation="MGGKIVFGVAVWLYGAIWCFQPIQGREAAESRSETVVSLQDNGL
                     PLTNPDMGWVLHYYDNVPANYGSRLEPSDTMDDFPSLSVVYLRIPWSYLEPQEGQFCW
                     SVLDTPAQRWISKGKRIALRISCSESWTRFATPEWVQQAGAKGYNFKVGTGVTDDGPY
                     WEPDYNDPVFLEKLDRFLAAMAARYDGNPEVAFIDVGSFGVWGEGHTFASTRIRYPAE
                     TVIRHIELYRKHFKKTLLVANDDFVMTENGEQAIAHALAAGLTLRDDSILVQARPKSY
                     FHAEMAQPFWPKWPVILECEHYGPSRGRGAWEDGTLYLRAVLEYHASYASIHWWPREF
                     LEANRDLIARINRYLGYRLIPTEVRWSSQV"
     gene            1473..2912
                     /locus_tag="PLD05_15325"
     CDS             1473..2912
                     /locus_tag="PLD05_15325"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOP78857.1"
                     /translation="MTKRFLGALATTLILAGVASAGNNVLPELPPLPPLDEISPPEIT
                     APATSPGLILPVGASDWTLPDRLNPSRELVTGMEKQAPAPLPAEPSAPREPVRVTRVC
                     PSSNQDAWTLPQPCFLQNLGIRMGGWLEQGITFNAQDPADHFNGPVATNDLDSEYQMN
                     QLWLWFDRPADNGGCGFAWGGHVDIMYGTDWRFGINHGLEDRINGFDRQTYGMVIPQF
                     YLELAYNRLSVKLGHFAGILDYEAVPGPMNPFYSHSYSYGYTVPQLVTGLLADYKLTD
                     QLSIQGGFHRGWMQFEDNNDELDFMGGVKWKSWNERLTLAYAVSAGAQDDAGDQDRFV
                     YSLVAQYQVNKRFKYVLVHNLGTENNALPNGDDAEWYGINQYFLYTINPCWSAGMRVE
                     WMRDDDGVRIAGPGNIPGVRAWSGRGFAGDFYEITWGLNWRPNGNWTVRPEIRYDWYD
                     GLAGPTGLPFDGGSKDHQLTVGLDAIFTF"
     gene            3205..>3724
                     /locus_tag="PLD05_15330"
     CDS             3205..>3724
                     /locus_tag="PLD05_15330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOP78858.1"
                     /translation="MALGEDRVVFQARKFQVVEKPVPNSPEPLRHVIRHRGAVAILAV
                     LEDGRLVFVKNYRISIDAELLEIPAGTREPGESPQEAAIRELKEETGYRARIWRQLCE
                     FYTSPGITDEKMTLFLAQSLEPGEPQLAPDERLEPCLLTLDEALAAIRSGTIQDAKTL
                     VAVLTYQNMREKD"
BASE COUNT          864 a         1047 c         1004 g          809 t
ORIGIN      
        1 cacttgggag gaccaacgca cttccgtagg aatgagccgg taaccgaggt agcggttgat
       61 ccgtgcaatc aagtcgcggt ttgcctccaa gaactcgcga ggccaccagt gaatcgaagc
      121 ataactggca tggtactcta aaaccgcccg caaatagaga gtgccgtctt cccaagcgcc
      181 acgtcctcgc gatgggccat agtgttcaca ttccaaaatg acgggccact tgggccagaa
      241 aggctgagcc atctccgcgt gaaagtatga cttcgggcgc gcttgcacca ggatgctgtc
      301 gtctcgaagt gttaaaccag ccgccaacgc gtgagcgatg gcctgttctc cgttttccgt
      361 cattacgaaa tcgtcattgg ctaccaaaag tgtcttttta aaatgtttgc ggtagagctc
      421 gatatgccgg attacggtct cggccggata ccgaatgcga gtgctagcaa atgtgtggcc
      481 ttcgccccag accccaaacg atcccacgtc gatgaaggcc acctcaggat tcccatcgta
      541 gcgtgcagcc atggccgcta agaagcgatc gagtttttcc aaaaagaccg gatcattgta
      601 atccggttcc caatatggcc catcgtctgt aaccccggtc cccaccttaa agttgtatcc
      661 ctttgctccg gcctgctgga cccattcggg cgtggcgaat cgtgtccacg attcggaaca
      721 gcttatccgc agggcaattc ttttgccttt ggaaatccac cgctgggccg gtgtgtccaa
      781 gacggaccag cagaattgcc cttcctgggg ttccaggtag gaccagggaa tccgtaaata
      841 aacgaccgat aggctgggaa aatcatccat cgtgtcggac ggttctaacc gtgatccata
      901 atttgcggga acgttatcgt aatagtgcaa aacccagccc atatcaggat tggtcagcgg
      961 gagcccattg tcttggagcg agaccaccgt ctcggatcgc gattccgcag cttcacgtcc
     1021 ctgaatcggt tgaaagcacc aaatggctcc atacaaccag acagcgacac cgaatacgat
     1081 ttttccgccc atatgtttgt tcctcgtttc tcgcggtgga ttatccggca aagggtgcgt
     1141 tcgcctctgt cttaacggtg cacaccctat atgccacaag agcgatgttt agacagagcg
     1201 aaagctcgcc cgctcccccc gaaatttccc accttctcta atcctcatat ccagacctta
     1261 accacggtca aaatcgctat tttccgcgtg gactacaaaa aacgcaacaa caaaccgacc
     1321 gggccaagcc gaaagtttga atgagcggcc caatgtcgcc ttgcgcaact tttgcggaat
     1381 gttcagacga actggtggca ctgagcgtgc aaggacctaa acgaatcacc atcttttccg
     1441 cagctagttt tttaccacgg agacacgctg ttatgactaa gcgatttctc ggcgccctag
     1501 caacgacatt gatattggcg ggcgtagcat ccgctggaaa taacgtgtta cctgaattgc
     1561 cgcccctgcc ccccttagac gaaatcagcc caccggagat aacggccccc gcgacaagcc
     1621 caggcctgat attgcctgtt ggggcatcgg attggaccct gcccgatagg ttaaacccca
     1681 gtcgcgaatt agtgaccggg atggaaaaac aagctcccgc gcctctcccg gcggaacctt
     1741 ctgccccccg ggaacctgtt cgggtcaccc gcgtatgtcc ctcctccaac caggatgcct
     1801 ggacacttcc gcagccttgt ttccttcaaa accttggaat ccgcatgggt ggatggttgg
     1861 aacagggtat cacattcaac gctcaggatc cggccgacca tttcaacggg ccggtggcca
     1921 ccaatgacct ggactcggag taccaaatga accagctctg gctgtggttc gaccgtccgg
     1981 ccgacaatgg cggctgtgga ttcgcctggg gtggccacgt ggacatcatg tatggcaccg
     2041 actggcgctt tggaattaat catgggctag aagaccgcat caacgggttc gaccgtcaaa
     2101 cctatggcat ggtcattccc cagttctatt tggaactggc ttacaatcgg ctatcagtga
     2161 agctggggca ctttgccggg attctcgatt acgaagccgt cccaggccca atgaatccct
     2221 tttatagcca ttcctactct tacggttaca cagtgccaca actcgtgacg ggcctgttgg
     2281 cggactacaa gttgaccgac caattgtcga ttcagggtgg attccataga ggctggatgc
     2341 aattcgagga caacaacgat gaactggatt tcatgggcgg tgtgaagtgg aaatcttgga
     2401 acgaacggct gactttagcc tatgccgtgt ccgccggagc gcaagatgac gcaggtgatc
     2461 aagatcgttt tgtctatagc ctcgtagcac aataccaagt gaataaacgc ttcaagtatg
     2521 tcctcgtcca caacctagga acggaaaaca acgcccttcc gaatggtgac gacgccgaat
     2581 ggtacggcat caatcaatat ttcctttaca cgatcaatcc ctgctggtcc gcaggaatgc
     2641 gtgtggaatg gatgcgggac gatgatggcg tccggattgc cggcccgggc aatattccgg
     2701 gagtccgggc atggagcggc cgtggatttg ccggggattt ctatgaaatc acgtggggtt
     2761 tgaattggcg tcccaatggc aattggaccg tccgtccgga gattcgttat gactggtacg
     2821 atgggctagc gggtcccacg ggccttccct tcgacggcgg ttcaaaagac catcagttga
     2881 cggtcggact ggacgccatt ttcaccttct aacgttccgg aacgagcgcg gtaccctcag
     2941 agccagaccg aaccgcgaag aggtgaccgt gaagcgccgc gtcattcgcg cggcgctttt
     3001 tgttgatggc taaatggcaa gcaagaatcg ttccggcggt gtagctcaca agcgggcaaa
     3061 aagtccaaag ccttctcgcc atgtcggaag tgccgggcct gtcgcttttc acacggccga
     3121 tgggcaacgt acactggggg ttcatcccgg aaaaacagca tgcatttttg aggacggtcg
     3181 ggagcattct cgcgcgagga ttatatggct ttaggggaag accgagttgt gttccaggct
     3241 cgcaagtttc aagtggtcga gaagccggtt cccaacagcc cggagccgct tcgccatgtg
     3301 attcgtcacc gaggagccgt ggcgatcttg gctgtgctgg aggatggccg acttgtgttt
     3361 gtcaagaatt accggataag catcgacgcc gagctcctcg aaatccccgc cggaacccgc
     3421 gaacccggcg agtctcccca ggaggcggcg atccgggagc tcaaggaaga aaccggctac
     3481 cgtgcacgca tttggcggca actctgtgag ttttacacat cccctggaat caccgacgag
     3541 aaaatgacgc tattcctggc ccagtcgcta gagcctggag agccgcaatt ggcacctgac
     3601 gaacgtctgg aaccatgcct cctgacgttg gacgaggccc tagcggcaat ccgcagtgga
     3661 accatccaag atgccaagac gcttgtagcc gttctgacct atcaaaacat gagggaaaaa
     3721 gatc
//
LOCUS       DAOSUT010000263         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10312_length_3698_cov_6.717815, whole genome shotgun sequence.
ACCESSION   DAOSUT010000263 DAOSUT010000000
VERSION     DAOSUT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10312_length_3698_cov_6.717815"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1784)
                     /locus_tag="PLD05_15335"
     CDS             complement(363..1784)
                     /locus_tag="PLD05_15335"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOP78859.1"
                     /translation="MIPSATNTFSTVRKVFSLTCGLLVLSGWLAQAEEPGVIQVGTAA
                     VVITPPLGTPMAGYYSARGSEGVIDDLYVKAMVLDDGQTQVALVSCDLISLPATVVRA
                     AREKIASQTSMPGDHVLISATHTHTGPALRRGSKRDELDGGASDLAQQYTNSLPEKIA
                     QVVAEAFKKRTPCRLAFARQEEHELSFCRRFWMKDGSVGWNPGKLNPNIIRPINPIDP
                     EVLVLYVETLKSEPVAAYVNFALHCDTVGGTLISADYPGVLAKRLAEYKGPQFVTLFG
                     NGTCGDINHINVHWKDRQKGPEEAGRIGTILAGDVMKALMKLQAVTDNRLAVRREIVP
                     LPLAPITEEDVKRAKEIIAQGSKAKFLEQVFAYKVLDVADRNGQPLEAEVQVITLGRD
                     VAWVALPGEIFVDLGLSIKAVSPFRQTFVVELANGSLGYIPNRSAYAEGQYEVVSARC
                     AEGSGEMLVNAAQRLLAEVYGGE"
     gene            complement(1865..2941)
                     /locus_tag="PLD05_15340"
     CDS             complement(1865..2941)
                     /locus_tag="PLD05_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOP78860.1"
                     /translation="MTRCYALRESSPQATALTRHVAVSQNVIPRREVLGYMAAFGSAF
                     VAAQGLVSNAEAEELSRQQEVPPPRKRYEMKKSINLWALPYPAKMSLRECLQLCKEAG
                     FEGVELNYALDGDLTPETSDAAVKDVAKMVRTIGLEVSGLCSFLFWPYSFTHQDPERR
                     RRGVELAIDMIRVAQLLGTKNLLVIAGSTYVPWLPDDPPVPFDVCERRSREAMLQILP
                     VAEKAGISLNVENIFMNGFLHSPQELVSYVDSFKSPNVGIHFDTGNIGQYHFAEHWIP
                     PLGSRIRNVHLKEVTKKVNEFHLHTFRPLLDGTTNWPAVLEALDQVGYRGYLTFEYFN
                     PYPHWPEALVFHTSDALDRMLGRK"
BASE COUNT          811 a         1125 c          977 g          785 t
ORIGIN      
        1 ggtaaggcga caacccatcc acgttgcccg tgctccgact cgcgggacgg ccttgatacc
       61 acagcgatca agccaattca taaaaggatt atcggaagac taaatcttgt caagaggaag
      121 aaatttcgcc gccatgcaga tttttagccc accgccgttc gcacatctcg cgacgccaca
      181 agaacagccg atccgtgcct cttttagagc ctatcccaca acctctcctc gcccgttcga
      241 cgggacaggg gctagggatg agggtggctc gaatcgctgc tagttttctc gattgactcc
      301 gacgatggct taccctcacc ccaaccctct cccacaggga gagggaattc tggaataggc
      361 tcttattcgc ctccatagac ctccgcaagc agccgctggg ccgcattgac caacatttca
      421 ccggagcctt cggcgcagcg ggcactcacc acctcgtact gaccctcggc ataagccgag
      481 cgattcggaa tatatccaag cgaaccgttg gccaactcca cgacaaacgt ctgtcggaat
      541 ggcgacaccg ctttaatgct gagtcccagg tcaacgaaaa tctcccccgg caacgccacc
      601 caggccacgt cacgccccaa ggtgatcact tggacctcgg cctccagcgg ctgaccgttg
      661 cgatcggcca cgtccagaac tttgtaggcg aagacctgct ccaggaactt ggctttgcta
      721 ccctgggcga taatctcctt ggcccgtttt acgtcctcct ctgtgatagg tgccaaaggc
      781 agaggcacga tttctcgccg aacagccagc cggttatcag tgaccgcctg caatttcatc
      841 aatgccttca ttacatcccc ggccaggatt gtcccaatcc gtcccgcttc ctccggccct
      901 ttttgccgat ctttccagtg gacgttgata tggttgatgt caccgcatgt cccgttgccg
      961 aacagcgtta caaattgcgg gcctttgtac tccgctaggc gtttcgccag cacgccgggg
     1021 taatcggccg agatcagcgt tcctcctacc gtgtcgcagt gcagtgcaaa attgacgtag
     1081 gccgccacag gttcgctttt gagcgtttcg acataaagga ccaaaacctc gggatcaatg
     1141 ggattgatag gccgaatgat attcggattc agcttgcccg gattccaacc tacgctccca
     1201 tccttcatcc aaaaccgacg gcaaaaagag agttcgtgtt cctcctggcg ggcaaaggcc
     1261 aaacgacaag gcgtgcgctt cttgaaggcc tcggccacca cctgggcgat cttctccggc
     1321 agactgttgg tatattgctg ggccaaatcg ctggcaccgc cgtccaattc gtcccgtttt
     1381 gaaccccgtc gcaaggccgg tccggtgtgc gtgtgcgtcg ctgaaatgag gacatggtcg
     1441 cccggcatgg atgtctgaga ggcaatcttc tccctcgccg ccctcaccac ggtggcagga
     1501 agactgatta ggtcacatga caccaaggcg acctgggttt ggccatcgtc caggaccatg
     1561 gccttgacat aaagatcgtc gatcacccct tcactcccac gggcggaata gtaacccgcc
     1621 ataggtgtcc ccagcggtgg cgtgatgacc acagcggccg tgcccacttg gataaccccc
     1681 ggctcctccg cctgggcaag ccaacccgaa agaaccagca gaccgcaggt caggctgaag
     1741 accttcctta ccgtactgaa cgtattcgtt gccgacggga tcattggtgc aactcctttt
     1801 tggcggagac gacatgctga gaggatcgcc ctccctgtca aggccgacgc gcaaaaccaa
     1861 ttccttattt ccttcccaac atccggtcga gggcgtccga tgtgtggaac accagggcct
     1921 ccggccaatg aggataagga ttgaaatact caaacgtcaa ataacctcga tagcccacct
     1981 gatccaacgc ttcgagaacg gccggccagt tggtggtgcc atcgaggaga ggtcgaaatg
     2041 tgtgcaggtg gaattcgttg accttcttgg tgacttcctt caggtgaacg ttgcggatcc
     2101 gcgaaccgag cgggggaatc cagtgctccg cgaagtgata ctgtccaatg ttgcccgtgt
     2161 caaaatggat gccgacattc ggcgacttaa aactatcgac ataactcacc agctcttgcg
     2221 gcgaatggag gaagccgttc ataaaaatgt tttccacatt aagggagatg cccgccttct
     2281 cggccacagg caggatttgc agcatcgcct cgcgtgatcg gcgttcacag acgtcgaatg
     2341 ggacgggcgg atcgtcgggc agccaaggaa catacgtgga gccggcaatg acgagaagat
     2401 tcttcgttcc gagcagttgg gccacacgga tcatgtcgat cgccagctcc accccgcggc
     2461 gtcgccgttc cgggtcctga tgggtgaagg aatacggcca aaataggaac gagcacaggc
     2521 cactgacttc cagcccaatg gtgcggacca tttttgcgac atccttcacc gcggcatcgg
     2581 aagtctcggg cgtaagatcc ccgtcgaggg cgtagttgag ctccacaccc tcaaaaccgg
     2641 cttctttgca aagttgcaag cactcccgga ggctcatctt ggccggatag ggcaaggccc
     2701 aaagattgat agactttttc atctcgtagc gcttgcgtgg gggcgggacc tcctgctgac
     2761 gggagagctc ctccgcctcc gcgtttgaaa ccagcccctg cgccgccaca aacgcacttc
     2821 caaaggccgc catatagcca agcacctccc gcctgggaat gacattttgg gacaccgcca
     2881 catgacgtgt caaagccgtc gcctggggag acgactcgcg caaagcataa cacctggtca
     2941 ttgtccgcaa ctcctgtatc cggtgaatcg cctaaacaaa cctccgcgtt agttcatccg
     3001 gccacgacaa agcgtggtcc tccatctcaa catgggaggg acgcggtcct ccgcgtcctg
     3061 ccgtttgaac ggacgcgctc tgtcgcgttc accgtgttat gccgtttgtt tgtcatactc
     3121 attggcaaca ccgattgtgt ccacatctca cgcggtaggc ttccgccgag aagttatcca
     3181 agccccttcg ccttccgaca cgacataccc gatggaccat gccccggcga ccgctcttat
     3241 tgtagccgtc cccaaattgt gagcaagaac gcaaaggtcc tcctaaacgg gtgcgcttgt
     3301 tcccagcagt gtcgatcccg tcctaccagt ccgagatcgc tgaagggaat ttgcgaaggt
     3361 tgcacaagcg agggaaatgt cccccgcgca gacgcttcgg cagggatcga cacagatttg
     3421 aggggcgatt tcaacgcggg aggaggcgta cctctggggc ggattctcgg attgggccca
     3481 ataaagaaac cgcctgaaac cgtccagggt tccaggcggt acacagtcaa aggcggccga
     3541 aatggtactt agtcctcaag attgaactgg aattcgttca ctttcccttc ctcaattttt
     3601 accttcaaag gtgtggagga ggcaaacttg tacttttgtg gtaaaaggtc cttgttgatc
     3661 atgacgtctt ccgtgccgcc ttcgggttta ggcacctt
//
LOCUS       DAOSUT010000264         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10331_length_3694_cov_9.036274, whole genome shotgun sequence.
ACCESSION   DAOSUT010000264 DAOSUT010000000
VERSION     DAOSUT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_3694_cov_9.036274"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLD05_15345"
     CDS             <1..881
                     /locus_tag="PLD05_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOP78861.1"
                     /translation="FRQLMYPCETVLHVYPDIKQLSEYAMLARVNRLSLIGVRRVRRV
                     GHEDEFERLRDYTIDDEYRWIDWRATARRRKLTVKDFQQTQSQRVVFMVDCGRMMTDE
                     WAGLSMLDHTWNAVLLLAYVALTHGDSVGLLAFSDEIHAFLNPRGGRHQMARLINMAF
                     DRFPRMVESRYDLAFRYLAAHVRKRTLVVLVTHLIDDVNGRQVERYLTNLAGRHLPLG
                     VFLRDHTMFAPLSDGQKERDDLWKAAAAARILTWRHQVITSIRSKGALVLDVFPEEMT
                     APLVNQYLEIKARRLL"
     gene            complement(859..1704)
                     /locus_tag="PLD05_15350"
     CDS             complement(859..1704)
                     /locus_tag="PLD05_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78862.1"
                     /translation="MVDLAQTRVVIRAREIGDLCDLALAAIRRDGWSLLRWWLLMVIP
                     YLVVVDSILVIFFRRLWEPEPEFSLIGYAFLISVAGLTPLSLLSGLLTLHLGMSVFVK
                     RPTFREVWRAWISRLGYMLLVQLMPFASRGPAIAEIVLLERLVPTTREGRAAIRRRLT
                     ALASYGLSINQLGAFFALLTFFIIGLTMAGMYFSFLTFETPWRWGWALVGFQLGACIA
                     GGFNRVVRFFLYLNSRIKYEGWDVELAARAAAQRLLRTNREVSSSQPLTPPTSNQRPK
                     AAVSP"
     gene            complement(1697..2656)
                     /locus_tag="PLD05_15355"
     CDS             complement(1697..2656)
                     /locus_tag="PLD05_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOP78863.1"
                     /translation="MVQVPDFLKQRQADWLELEHICHIANRVTERPTAEMVCRLSELY
                     RAACADLALAEAQQLSPEVLDYLHRLVGQAHNVLYRHPQSLPRQWLRAIFIEVPAYAI
                     RDKMIWLAFASFWGLFLLTAFLSRQSETFAEAVVGQELLDAIEDMYSRPFSDEYPTIV
                     AVGFYIMHNIGIALRCFALGILFVLPGLFILVFNAVTLGTVFGHMSVAAQADTFFEFV
                     TGHGPFELTGICLAAGCGMRLGFSLLETGELTRLASLRLAARQVAPVLLVACFFFVLA
                     AIIEGLVSPSATPVETKRWIATLSTVVLVVYFSTGFFTRPSHG"
     gene            3366..>3694
                     /locus_tag="PLD05_15360"
     CDS             3366..>3694
                     /locus_tag="PLD05_15360"
                     /inference="COORDINATES: protein motif:HMM:NF040121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HOP78864.1"
                     /translation="MDYDDREVPSSCEHFLFVTGQLAEPLLNRMLPDLATAFEFQYSV
                     AVMPITIAGLMSVPWISERLKVPPKVTRILLPGRCQGDVGELSRRTGIPAERGPNDLH
                     ELPDFFKR"
BASE COUNT          853 a         1053 c         1078 g          710 t
ORIGIN      
        1 ggtttcggca gttgatgtat ccctgcgaga cggtcctcca tgtctatcct gatattaaac
       61 agctttccga atacgccatg cttgcccggg tgaaccggct cagtctcatc ggcgtccggc
      121 gtgttcgccg tgttgggcac gaggacgaat tcgagcgcct gcgggactac accatcgatg
      181 acgagtatcg ctggattgac tggcgcgcca ccgctcggcg gagaaagctc acggtcaagg
      241 acttccagca gacccaaagt cagcgcgttg tcttcatggt ggattgtggt cgaatgatga
      301 ccgacgaatg ggccggcttg agtatgctcg accacacctg gaatgccgtg ctgctattgg
      361 cttatgttgc cctaacgcat ggcgactctg tggggctgtt ggccttctcc gatgaaatcc
      421 acgccttttt gaatccgcgt gggggaaggc accagatggc gcggctcatc aacatggcct
      481 tcgatcgctt tccgcggatg gtggagtccc gttatgacct ggcgttccga tatttggcgg
      541 cccatgtccg aaagcggacc ctcgtggtgc tcgtcacgca cttaatcgac gatgtcaacg
      601 gccgacaggt ggaacgttac ctcaccaatt tggcgggacg gcacttgccc ctgggggtct
      661 tcctccgcga tcacacaatg ttcgccccac taagcgacgg gcaaaaggaa agggacgacc
      721 tatggaaggc ggcggccgcg gcgcgcatcc tcacctggcg tcatcaggtc atcacatcga
      781 tccgcagtaa aggggcactc gtgctggatg tgtttccgga agaaatgacg gccccgctcg
      841 tcaatcaata ccttgagatt aaggcgagac ggctgcttta ggacgttgat tggacgtagg
      901 cggagtgagc ggctgagaag aggagacctc ccggtttgtc cgcaggagtc gctgggcggc
      961 tgcccgggcg gccagctcaa catcccaacc ctcgtacttg atccgactgt tgagatacag
     1021 gaaaaaccgc accacgcggt taaacccccc ggcgatgcac gcgccgagct gaaaaccgac
     1081 aagggcccaa ccccaacgcc agggagtctc aaacgtcaaa aaggagaagt acattcctgc
     1141 catggtaagt ccgataataa aaaacgttag caaggcaaaa aaggcaccaa gctggtttat
     1201 ggataaaccg taactggcta aggctgtaag tcttcgccgg atggccgcgc gaccttcccg
     1261 cgtggtgggc acgagtcgct cgagtaaaac gatttcggca atggcagggc cgcgactcgc
     1321 aaagggcatg agttgcacca ggagcatgta accaagccga gagatccacg ctcgccaaac
     1381 ttctcgaaac gtgggccgtt tgacgaaaac gctcatcccc aagtggaggg tcagtaaacc
     1441 gctgagcaaa gaaagcggcg tcaggcccgc cacgctgatg aggaaagcgt atccgatgag
     1501 ggaaaactcg ggttccggct cccaaagtcg acggaaaaaa atgacgagga tcgaatcgac
     1561 caccaccaga tagggaataa ccatcaacag ccaccagcgg agaagcgacc aaccatcgcg
     1621 gcggatcgcc gccagggcga gatcgcagag gtcgccgatc tcccgcgcgc gaataaccac
     1681 ccgtgtctgt gcgagatcaa ccatggctcg gtctggtgaa aaaacctgtg gagaaataga
     1741 cgacgagcac gaccgtggaa agcgtggcaa tccaacgttt ggtctctacc ggcgtggccg
     1801 acggcgacac gaggccttcg atgatggcgg ccaaaacgaa gaagaaacag gccaccaaca
     1861 gcaccggggc cacttgtcgg gcggcgagcc gtagggaagc cagccgcgtc aattccccgg
     1921 tctctagcag cgaaaagccc agccgcatgc cgcatcccgc cgccaagcaa atccccgtta
     1981 attcgaaagg cccgtgtccc gtgacgaatt caaagaaggt gtcggcctgg gcggccacgc
     2041 tcatgtgccc aaaaaccgtt cctaacgtca cagcgttgaa caccaggata aacaaccccg
     2101 gcaacacaaa caggataccc aaagcaaagc accggagagc aatcccgata ttgtgcatga
     2161 tataaaaccc aactgccaca atcgtcgggt attcgtcgct gaacggccga gagtacatat
     2221 cctcaatggc gtcaagcaac tcttggccca caacagcctc tgcgaaggtc tcactttgtc
     2281 gggaaagaaa agcggtcaac aagaaaaggc cccaaaacga ggcaaaagcc agccaaatca
     2341 tcttgtcgcg aatggcgtag gctggaacct cgatgaagat cgcgcggagc cactgccggg
     2401 gtaggctctg cgggtgacgg taaagcacat tatgggcctg ccccacaagg cgatgtagat
     2461 aatccaacac ctccggcgat agctgctgag cctcggctag tgccaggtcc gcgcaggccg
     2521 cccgataaag ctccgacaac cggcagacca tttcggcggt tggtcgctcc gtcacgcggt
     2581 tggcaatgtg acaaatgtgt tccagttcga gccagtccgc ctgccgttgc ttgagaaaat
     2641 ccgggacctg taccatggca tcacagtgtc caacaaggaa acttcatgag ggactacttt
     2701 gacttagagc gtatcccaca acctcccctc tctcgttggg cggaagaggg gccgggggtg
     2761 agggtggctt gaatcgctgc cggttttctc aattgactcc gacggtcgct tgccctcacc
     2821 ccaaccctct cccaagggga gagggaactt tgggataggc ccttgtggcg agatgctact
     2881 ctttcagcct tcaaccgaaa acgagatccc cgccgcggat tgccacgcaa aaccaaatcc
     2941 acgctgaaga tttattgtcc cttaaccgtg ggcaattttc gagccatcat ccaccggcgg
     3001 ctcggggatt ttaccggtgg ggaatggggg cttgtctctg ggttgtgttt agctatcttt
     3061 acagaggttc cccaatttat ctacgtctcg ccgtgggggc tgcgtagaat agagagtagt
     3121 agcggacaag catcaccggc ggacaggcca ggcaaccgga gcttgccgcc caagtccggg
     3181 gtgaccgtgg ggtcactgcc ggggtacgga tgtttttgga caacatcgcc ctgactggcg
     3241 tcggtggcgg tcacccctcg ccgaattggt ggccagagag cgttgtcggt caacctgtaa
     3301 ctgttttgtc gaatataacg ccaatcatcc cgcccaaaag cggctaggcc gcggaggcgt
     3361 cgtttatgga ttacgacgat cgtgaagttc cttcgtcttg tgaacatttc ctctttgtga
     3421 cgggtcagtt ggccgagccc cttctcaacc gaatgttgcc ggaccttgcg acggcgttcg
     3481 agtttcagta ctcggtggcc gtcatgccga tcacgatcgc tgggctgatg agcgttccct
     3541 ggatcagcga gcggttgaaa gtgccgccga aggtgacgcg gattcttttg cccggtcgtt
     3601 gtcaaggcga tgtgggcgaa ctatctcggc ggacgggcat cccagcggag cgaggtccga
     3661 acgatcttca cgagttgccc gatttcttca agcg
//
LOCUS       DAOSUT010000265         3692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10341_length_3692_cov_8.772890, whole genome shotgun sequence.
ACCESSION   DAOSUT010000265 DAOSUT010000000
VERSION     DAOSUT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10341_length_3692_cov_8.772890"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..689)
                     /locus_tag="PLD05_15365"
     CDS             complement(87..689)
                     /locus_tag="PLD05_15365"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOP78865.1"
                     /translation="MFSGIVEALGTVVDVISEPPGVRLIVKEPRIAAETRVADSIAVN
                     GCCLTVIDVQGETFAFQAGPETLSRTNLGDLKPGSRVNLERSLKVGDRLGGHFVTGHI
                     DDVGFLAERQDFGEWSRFWFRIPRRLAKQMAPKGSIAVDGVSLTIVDSEPDRFSVALI
                     PYTLAVTTLGPMKVGDKVNLETDILAKYVERQLEAQHWVS"
     gene            complement(980..1183)
                     /locus_tag="PLD05_15370"
     CDS             complement(980..1183)
                     /locus_tag="PLD05_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78866.1"
                     /translation="MATQPMGANIVAISTCQASGTGLLAWVAKHFSNTVRIVLTGQPD
                     LPTAMKAINEGQVFRFLTKPCPE"
     gene            1182..1790
                     /locus_tag="PLD05_15375"
     CDS             1182..1790
                     /locus_tag="PLD05_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOP78867.1"
                     /translation="MPDEIVKKAFRGEPLKENEVATYQTHPRIAHDLISKIPRLEQVA
                     RIVAYQEKLYNGGGFPADLVEGDDIPLGARIIKLAFDWDSLISGGLSPELALAEITDR
                     RGWYDPKVVNALRRILNVHQVCVVREMKISDLVDRLVLADNVYSVNGTLLCSKGQEVT
                     PAIRFRLRNYAVNVGIARPIRSFVPTVGAVNELSALSEPNSE"
     assembly_gap    1862..1961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1962..3014)
                     /locus_tag="PLD05_15380"
     CDS             complement(<1962..3014)
                     /locus_tag="PLD05_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78868.1"
                     /translation="MVCRSSIFCALVISLLSCPMASVAEEKGHPNIILVFIDDMGWGD
                     FSCFGNTEANTPNVDAMAAEGIRFFQFYVNSPICSPSRCAISTGQYPARWRITSYLNN
                     RADNARRGVANWLDPAAPMLARILQAHGYATGHFGKWHLGGQRDVDDAPPITAYGFDE
                     SLTNFEGMGPKLLPLTQKPGQQGYGRIWADAEILGGPVIWMPRSQITGGYVNAALAFI
                     RRAMRAGKPFYVNVCPDDVHAPFWPPVDKWGDGSRRQLYRAVLEEMDRQLGPLFDFVR
                     KSPELRENTLIIICSDNGPDVNAGSSGPFRDGKATLYEGGVRSPLILWGPGLVVKEAA
                     GTWNKSSVFSAMDLVPS"
     gene            3365..3448
                     /locus_tag="PLD05_15385"
     tRNA            3365..3448
                     /locus_tag="PLD05_15385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3399..3401,aa:Leu,seq:gag)
     gene            3619..3692
                     /locus_tag="PLD05_15390"
     tRNA            3619..3692
                     /locus_tag="PLD05_15390"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3653..3655,aa:Lys,seq:ctt)
BASE COUNT          808 a         1081 c          947 g          756 t
ORIGIN      
        1 ccggttttct caaccgattc cgacgatcgc ttgccctcac cccaaccctt tcccaaaggg
       61 agagggggaa tttttgggat agactcttag cttacccaat gctgggcctc cagttgacgc
      121 tccacatatt tggccaggat gtcggtctcg agattgacct tgtcgccgac tttcatgggg
      181 ccaagcgtcg taacggccaa ggtgtacgga ataagggcca cactaaagcg gtccggttcg
      241 ctatcgacaa tcgtcagact aacaccatcc acagcaatgg agcccttggg ggccatttgt
      301 ttggcaagac gccgagggat gcggaaccag aaccgcgacc actcgccgaa gtcctgtcgc
      361 tccgccaaaa aaccgacgtc gtcaatgtgg cccgtcacaa aatgtccacc taaccgatcc
      421 cctaccttaa gggaccgctc caaattgact ctacttccgg gtttaagatc gccaagattc
      481 gttcgagaaa gcgtttcagg acccgcttga aaggcaaagg tctccccctg cacgtcgata
      541 actgtcaggc aacagccatt gaccgcaatg ctatcggcca cacgggtctc cgcagcgatc
      601 ctcggctctt tcacaatgag tcgcacaccc gggggctccg aaatcacatc aacaactgtc
      661 cccaaggcct cgacgatccc agaaaacatg gttcgctctc ctctcaggcc gtgttggaaa
      721 tccctacgat gctattcttt gggggtgtta tccccaccac cctcgtaccg atgggtatct
      781 ctagactatc tttgatgcta aggtctgctt gcagaattga ccagccgcac cgcgcgatcg
      841 tcgcatttca ggccgggaaa agcgctccgt agggctccga tttaattacg cagtccacct
      901 agttctcggg ccctcagcgg ggaaaactgg tttgggcctc gaggacggcc aaaccttcgt
      961 gaatggcaga aatcagatct cactctggac aaggctttgt caaaaagcga aagacctggc
     1021 cctcgttgat ggccttcatg gcggtcggca aatccggctg cccggtcaag acgattctca
     1081 ccgtattaga aaaatgctta gccacccagg cgaggagccc cgtccctgac gcctggcaag
     1141 ttgagatcgc cacgatgttg gcccccattg gttgtgtggc catgcctgac gaaatcgtca
     1201 aaaaagcctt ccgcggcgaa cctctgaagg agaatgaagt cgcgacctac cagacccatc
     1261 cacgaattgc ccacgacttg atttcaaaaa ttccgcgact ggaacaggtg gcgcggattg
     1321 tggcctacca ggaaaagctt tacaacggcg ggggttttcc ggcggacctc gtggaaggcg
     1381 acgacatccc cttgggcgca cgcatcatca aactggcgtt tgactgggac tcgctcatta
     1441 gtggcggtct cagcccggaa ctggccctcg ctgagatcac cgatcgccgt ggttggtatg
     1501 atcccaaggt cgtcaatgcg ctgcgaagga tcctcaatgt ccaccaggtt tgcgttgtcc
     1561 gagaaatgaa gatttctgac cttgtcgatc gactggtcct cgccgataac gtttactccg
     1621 tcaacggcac actgttgtgc agcaagggac aagaggttac cccggccatt cgttttcgtc
     1681 tccgaaatta cgcggtcaac gtgggcatcg cgagacccat tcgctcgttc gttcccactg
     1741 ttggagcggt gaatgagctg agcgcgctat ccgaacccaa cagcgagtaa tcgcgccttt
     1801 cctttctgcg agcccccacc aatgccgcgt gcgttgctcg gtacccaaaa gaaaaaagcc
     1861 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgaagggac caggtccatc
     1981 gccgaaaaaa cagaggactt gttccacgtg cctgcggcct ctttcaccac caagcccggt
     2041 ccccacagaa tgagtggcga ccgaacgcca ccctcgtaga gtgtcgcctt accatcccgg
     2101 aacggaccgc tgctgcccgc attaacgtcg gggccgttgt ccgagcaaat gatgatcaac
     2161 gtgttctcgc gcaattccgg cgacttgcgg acaaagtcaa acaggggtcc taactggcga
     2221 tccatttctt ccaaaaccgc ccgatacaat tgccgccgac ttccatcccc ccatttgtca
     2281 actggcggcc aaaacggcgc gtggacatcg tcgggacaca cattcacata aaaaggtttg
     2341 ccggcgcgca tggcacgtct gataaacgcc aaggccgcat tcacataacc gcccgtaatt
     2401 tgcgaccgcg gcatccaaat gaccgggcca ccaaggattt cggcatcggc ccaaatccga
     2461 ccatatcctt gctggccggg tttttgcgtg agcgggagga gtttcggtcc catcccctca
     2521 aagttggtca gtgattcgtc gaatccatag gcggtgatgg gcggcgcgtc atccacatcg
     2581 cgctgtcctc caaggtgcca cttcccaaag tgccccgtgg cgtaaccgtg ggcttgaagg
     2641 attcttgcca gcatcggcgc ggcgggatcg agccagttcg ccacaccgcg gcgggcattg
     2701 tctgcccgat tgttgagata ggatgtgatc cgccaccgtg ccggatattg ccccgtgctg
     2761 atggcgcacc gagaggggga gcaaatcggc gagttgacat aaaactggaa aaagcgaatc
     2821 ccctcggcag ccatcgcatc gacgttcggg gtgttcgcct ctgtattccc aaagcaggag
     2881 aaatcgcccc agcccatatc atcaatgaac acgaggatga tattagggtg gcccttctcc
     2941 tcggccacac tcgccatcgg gcaagataac aagctgatga ccaatgcgca gaatatcgac
     3001 gatcggcaca ccatgagtcg ttcctcccgc gaaaatcaac ccgatcggtt aggcgtcata
     3061 gctcaccata accttatccc atctcgtttt ccccgtccac acgagggaac cgcggttgct
     3121 ttttcacccc atcaatgggc aggcacgagc tccgaccgca accgggccat gtccgtcacg
     3181 gacttgagac ccgtggcaag ccgggggtac tactcgatcc acgatacctg ctccaattcg
     3241 ccgacaaatg gtcaggtgga caacccgcca gacggctccc gacgggtatc agggacttga
     3301 cgttcaccac gtcaggttgc tagattaaaa gcaaaaggtc ctaaagacat tctgtaccaa
     3361 ctctgcggga atggcggaat tggcagacgc gctaggttga gggcctagtg gccgcaaggc
     3421 cgtggaggtt caagtcctct ttcccgcact tttgtttttt tggtgatgat agttcgccgt
     3481 tcgacgtcgt tcgtccagcg taccgtcaag ttttgctgcg aattttttga actcaccgta
     3541 gtcggtggcc agggatttga cggttcagct tgaccgtacg gtcgtcggat ggcaacggaa
     3601 tggaaatcaa cacaagccgc ccccttagct cagcccggca gagcagctga ctcttaatca
     3661 gcgggtcgta ggttcaaatc ctacaggggg ca
//
LOCUS       DAOSUT010000266         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_10864_length_3531_cov_8.808976, whole genome shotgun sequence.
ACCESSION   DAOSUT010000266 DAOSUT010000000
VERSION     DAOSUT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10864_length_3531_cov_8.808976"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PLD05_15395"
     CDS             <1..1196
                     /locus_tag="PLD05_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOP78869.1"
                     /translation="RALSDASLIAVACDHIIMHPDARIGGEGDGVFTEEEKAQVRRAL
                     REIICKRTGRSWSLPTALIDCDIEVFQVTRKGLPGFSECFSEEELNEAADRGTWVKKE
                     MITTKGQPLQLDGKKAVEYGLARDLVESFSDFKQLYGLERDPDLLEPTWIDTLVRALA
                     SPYLAVLLLIIGGLSLLIEVQTPGIGVGGFLAAVCFVLFFWSRFLGGTVGWLEVLLFA
                     LGVICLLLEVFVLPGFGVFGLGGGLLILISLILASQTFIVPQNTYQWLQLQQSLLILV
                     LAAIGIGVLIGVLNRMLPKTPGLKHLVLAPPSDQDREQMERRRSLLNVDESWIGMKGV
                     TVTVLLPSGKARFGKSVLDVISQDGDPIDPNTPVVITDVRGNRIYVQPKDLDAPSAAD
                     AIERL"
     gene            1322..1903
                     /locus_tag="PLD05_15400"
     CDS             1322..1903
                     /locus_tag="PLD05_15400"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOP78870.1"
                     /translation="MNTWLWPVLLMLTGLMLGILELFFVSHGILTFLSLSAVVASIVT
                     GFMYDTGLGVALLLVSVFGIPAFVILAVYLWPKTPVGRRVMLPAPDEDETMPDNPKLR
                     ELRGLIGRVGKTKGPMLPAGVVIIDGRAYDAVSNGFPIEANQWVRVVDVFGSWITVRA
                     LEKDEQPVDETPDDKSWKDRPITDVGTDPFAET"
     gene            1986..3062
                     /gene="floA"
                     /locus_tag="PLD05_15405"
     CDS             1986..3062
                     /gene="floA"
                     /locus_tag="PLD05_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909067.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOP78871.1"
                     /translation="MNLFFAQQGQNDLVQIIGFVLVLAAIIVGLVFLAVFFKFFRLWI
                     QSKTTGARIGILDLLGMTFRKVRPEVIVRAKIMAVQAGITEQDGVTTRALEAHYLAGG
                     DVNKVIRAMIAARNAKMTELDFRLATAIDLAGRDVLEAVQTSVYPKVIDCPKKGSGKE
                     SLEAVARNGIQLKVQARVTVRANILQLIGGATEETIIARVGEGIVSAIGSAESHYEVL
                     ENPDRISKTVLARRLDRQTAFEIVSIDIADIDVGQNIGARIQADQAEADMRVAQAKAE
                     GRRAMAVAAEQENLALIEEARATLVKAEAEVPRAIAEAFETGKLGIMDYYRLKNIQAD
                     TDMRTAISRMGLPLPQQGRTPQAG"
     gene            3107..>3531
                     /locus_tag="PLD05_15410"
     CDS             3107..>3531
                     /locus_tag="PLD05_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78872.1"
                     /translation="MLVFLGVFQLNLPVADATQLLVALAIIIFSILGSLLQSRQEAKK
                     RQERMERPRGRGRGPDTIEEMLRESSRPTPSRPQKRPSPPKPPPVVEAEVVETSIAEH
                     VTQTFQTTMARGEPVAGEPGSLAREKRAERKAGRRPRLET"
BASE COUNT          759 a          837 c         1056 g          879 t
ORIGIN      
        1 cgcgtgcctt atccgatgcc tccctgattg ctgtggcttg tgatcacatt atcatgcatc
       61 ctgatgctcg gattggcggc gagggcgatg gcgtgtttac tgaagaagaa aaggcccagg
      121 tgcgccgtgc actgcgagag atcatttgta agagaaccgg ccgttcgtgg tccctaccga
      181 cggcacttat tgattgtgac attgaggtct ttcaggtaac gcgcaagggg ctaccgggat
      241 tttccgagtg cttcagcgag gaggagctta acgaggcagc cgaccgtggg acatgggtta
      301 agaaagagat gatcaccacc aaaggacaac cccttcaact ggatggcaag aaagcggttg
      361 aatatggact tgccagagac ttggtcgaaa gcttttctga ttttaagcaa ctttacggtt
      421 tggaacgcga cccagacctc cttgagccaa cgtggatcga cacgctcgtt cgagcgctag
      481 catcccctta tttggcagtt ctgctcctca tcattggcgg gctctcgcta ttgattgagg
      541 tgcaaacgcc aggcatcggc gttggcggtt ttttggccgc cgtttgtttc gtccttttct
      601 tctggagtcg gtttctcggc ggaacggtgg ggtggcttga ggtgctgttg tttgccttgg
      661 gtgtgatttg tcttttgctg gaggttttcg tcctccctgg attcggggta ttcggcctcg
      721 ggggcgggtt acttattctg atttcactta tccttgccag tcagaccttt attgtcccac
      781 aaaacactta ccaatggctg caactccagc agtcgttact catcttagta ttggcagcta
      841 ttgggatcgg agttctcatt ggggtactga atcgtatgtt gcccaagacc ccaggactga
      901 aacatctggt gttggcgccg ccgagtgatc aggaccggga acagatggaa cgtcggcgga
      961 gcttgcttaa cgtcgatgag tcgtggatcg gtatgaaagg ggtcacagtg acggttctgt
     1021 tgccgagtgg gaaggcacgc ttcggaaaaa gtgttctcga tgtcatctct caagacggcg
     1081 accccattga cccgaacact ccggtggtca ttactgatgt cagaggaaat aggatttacg
     1141 ttcagcctaa ggatttggac gccccatcgg cggcggacgc gatcgaacgg ttgtgagtcc
     1201 cggttgcccg gtctgcttgt ggtttgccgc ggagggtgat agattaatga ccggagggct
     1261 cagagagact aatgacagta gccgccaatt tgtcacaatt gtctcacgat gggagttggg
     1321 aatgaacaca tggctctggc cggtgcttct catgttgacc ggcttgatgc tgggcattct
     1381 ggagttgttt ttcgtctctc acggcatttt gacgtttttg tccttgtccg ccgtcgtggc
     1441 atccattgtg accggattta tgtatgacac aggattgggt gtcgccctct tgcttgtgag
     1501 tgtttttggg atccctgcgt ttgtgatttt ggccgtttac ttgtggccaa agacccccgt
     1561 cggtaggcga gttatgttgc cggcacctga cgaagatgag acaatgcccg ataatcccaa
     1621 gttacgtgag ctgcgtggct taattggtcg ggttggcaaa acgaaagggc cgatgttgcc
     1681 ggcgggtgtc gtcatcatcg atggacgagc gtatgatgcg gtgagtaatg gttttccgat
     1741 cgaggcgaac caatgggttc gtgttgtgga tgtatttggc tcgtggatta cggtccgggc
     1801 cttggaaaag gacgaacagc ctgttgatga aacaccggat gataagagct ggaaagatcg
     1861 gcccatcacg gacgtaggta cggatccttt tgccgaaacg tgagcgcgaa ctggggtaag
     1921 accgtaagat aagtggaaat ctttcgggta agttttttcg acattagcta atgaaggata
     1981 acaagatgaa tctttttttc gcacaacaag ggcaaaacga cttagtccag atcattgggt
     2041 tcgttctggt tcttgccgca attattgttg ggcttgtctt tctggccgtg tttttcaagt
     2101 tcttccgttt gtggattcag tcaaagacga cgggagcacg catcggaatt ttggacttat
     2161 tgggaatgac gttccgcaag gtccggcctg aggtgatcgt acgggccaag atcatggccg
     2221 ttcaggcagg aattacggaa caggatggcg tcaccacacg ggccttggaa gcgcattatc
     2281 tggccggtgg agatgtcaat aaggtcattc gcgctatgat tgcggccagg aatgccaaaa
     2341 tgacggagct cgattttcga ctggccacgg ccattgacct tgcgggtcgg gacgtcttgg
     2401 aagccgttca aacaagcgtc tatccgaagg tgattgactg tcctaagaag ggttccggta
     2461 aagaatcgct agaggcagtg gcacgcaacg gtattcagct caaggtgcag gcccgtgtaa
     2521 ccgttcgtgc caacatcctt caactcatcg gaggtgcgac ggaagagacc atcatcgctc
     2581 gggtagggga gggaattgtc agtgccatcg gttcagccga gagccattac gaggtcctcg
     2641 agaaccctga ccggatttct aagaccgttt tggcccggcg tcttgaccgg caaacggcgt
     2701 tcgagattgt ctcgattgat attgccgaca ttgacgtagg acaaaatatc ggcgcccgta
     2761 ttcaggccga tcaagccgag gcggatatgc gggtggccca ggccaaagct gagggaagac
     2821 gtgccatggc cgtggcagcg gaacaggaaa acttggcctt gatcgaagag gcccgtgcta
     2881 cgctggtgaa ggcggaggcc gaggtccccc gcgcgatcgc cgaagcgttt gagacgggca
     2941 agctcggcat tatggattat taccgtctga agaacattca agctgacacc gatatgagaa
     3001 ccgcaatctc ccgtatgggg cttcctttgc cgcagcaagg gcgaacaccg caagcgggat
     3061 aaatgattga cggttgatgg cgtcctttta tgggaggcat gttccgatgt tggttttttt
     3121 gggcgttttt caactgaatc tgccggttgc tgatgctacg caactgcttg tcgccctcgc
     3181 cattattatc ttttctatcc ttggctcttt gctgcaaagc cggcaagaag caaaaaagag
     3241 acaggagcgc atggagcgac cgcgaggtcg tggtcgtggc cccgatacaa tcgaggagat
     3301 gttgcgagaa tccagtcggc caacaccttc tcgtccgcag aaaagaccgt cccccccgaa
     3361 acctccgcct gttgttgagg ctgaagtcgt cgagacaagc attgctgaac atgtcacgca
     3421 aaccttccaa acaaccatgg cgcgtgggga gcccgttgcc ggtgagccag gttcgttagc
     3481 tcgcgaaaag cgggccgaac gcaaggcagg tcgccgacct cgccttgaaa c
//
LOCUS       DAOSUT010000267         3475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11094_length_3475_cov_10.369006, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000267 DAOSUT010000000
VERSION     DAOSUT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11094_length_3475_cov_10.369006"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLD05_15415"
     CDS             complement(<1..258)
                     /locus_tag="PLD05_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78873.1"
                     /translation="MAKMMAGITWDPPSQAKRWGGLTNVTGTVNMLRPPVGILLVFLG
                     ISVFLSQAGCRKAPQAPALEEIEALPTETAQTPAPPPVTPVL"
     gene            677..1597
                     /locus_tag="PLD05_15420"
     CDS             677..1597
                     /locus_tag="PLD05_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78874.1"
                     /translation="MIARARLVTLVAVLCLGVAETASAQCLLSRLFNRETTYTTQYGG
                     YNIGYAYMPVVASPVATPVVSTTSYRVTYLPITPGATTVYRPAPVLYETPVVSTSYLP
                     VVSPYATPPAVSTPAVPTVAYRPVQIVYEPRRLFNWRPFANLRARRAGYWPGVAAPTW
                     STAYLVTYPSLPVVSDCTVCGPAPTAGSYAASYTVSSDSTLGCPSCAPASSSPLQPSV
                     DTSIPTLPSASPSDQPPATFKQQSSSTTGSSFRPADSTSGGMNATGTSVPVNETKPLV
                     RQALYEKPIPLVEEVADPPMVDVTGWRRSQ"
     gene            complement(1623..2798)
                     /locus_tag="PLD05_15425"
     CDS             complement(1623..2798)
                     /locus_tag="PLD05_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOP78875.1"
                     /translation="MRRPVIRSQALLIGPVLALVCVGISPAWCQEGPSFVIGNEFLRR
                     LDTPTGIFWTDVPLREGLLRLGRTHQVAIVLDRRCDPDQELTLTVRDRPLREVIAAIA
                     EACGVQSVVVSPIVYVGPPEAVQRLESLMVARRQEALKLGKVGLAGLRPLAVSWPRLT
                     TPRQLVTLWLQEANWRLTNPDEILHDLWPEGSLPPLTLVDRLTLVLAQFDRTFELNES
                     GEIRIVPITNTMAVARQFSVGPQARILAQQWQKMFPDCKVTTFGQDVVVEGPRETLDK
                     LADLLRTQKGPTAPRGSDGPPPTTPEARDPFEQRRFTVREGRGTLEGVIRQLAQQLEM
                     QVDFNRQSAIEAGIRLDQPIRFQVQNGTIDELWRAVLDPHGLTFRRVGRSITIYPKR"
     gene            complement(3224..>3475)
                     /locus_tag="PLD05_15430"
     CDS             complement(3224..>3475)
                     /locus_tag="PLD05_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOP78876.1"
                     /translation="AGVYGMNFDPDVSPWNMPELRWYLGYPFALALMLALAGVMIWSF
                     YRRGWIGQEWPLAPPNNHGTPDTPRAMAQADSPPQDHLL"
BASE COUNT          754 a         1003 c          978 g          740 t
ORIGIN      
        1 caaaacaggt gtcaccgggg gtggggccgg tgtttgcgca gtctcagtag gcagtgcttc
       61 aatttcctcc aaggccgggg cttgcggggc cttccggcaa cctgcttgcg aaaggaagac
      121 ggatattcct agaaaaacaa gcagaattcc gacagggggc cgaagcatgt tgaccgttcc
      181 cgtaacgttg gtcaagccgc cccaccgttt cgcctgagag ggaggatccc acgtgatgcc
      241 cgccatcatt ttggccatag cccatctccc gtttcaaaat attaaaaaaa ggatcaaccg
      301 cctgctgagt attgtctttc gcggattgtc atccgtcaag ttttttgcag gacaaaacgg
      361 cgtggcgtga gaccgcaagc cgaacttggg actccacctc tcgggaagag ggttccggca
      421 acgtaggcaa agaatatggt ggcgcatccg ttaacgctgc aaatattgga gcaatcggtt
      481 tattcggtag tttttgtcta agccgcacaa aaacacatcg ccttttttcg tacttgacaa
      541 atgtcatttc ggggacaagt tttattaaag gtgtgcgctg ctctgcgtgg catgtgtcgc
      601 gcgcagagtg atttagtggt taaggatacg aaccgggggc gatgccccat ggatttttgg
      661 tcgggagcga aatcgcatga tcgcgagagc aagactcgta acactggtag cagttctttg
      721 tttgggcgtg gcagaaacgg caagtgccca atgtctgtta agccgcctct tcaaccgtga
      781 gacaacctac acaacacaat atggcggata caacattgga tatgcctata tgcctgttgt
      841 ggcctcaccg gtggcaacgc ctgtggtctc cacaacaagc tatcgcgtca cgtatttgcc
      901 cataacccct ggtgcgacga cagtttatcg cccggcacca gtgctttacg aaaccccggt
      961 cgtcagcacg agttacttgc ccgtcgtttc gccttatgcg acacctccgg cggtatcgac
     1021 cccagccgtt cccacggtgg catatcgacc cgtgcagatc gtctatgagc cacggcgact
     1081 cttcaactgg cggccgtttg ccaacctgcg tgccaggcgg gcaggttatt ggcctggggt
     1141 ggcggcacca acctggtcaa cggcttattt ggtgacgtac ccctcgcttc ccgtggtgag
     1201 cgattgtacg gtttgtggac cggctccgac cgcgggtagc tatgcagcaa gttacaccgt
     1261 atcaagcgac agtaccctcg gatgtccctc atgtgcccct gcctcgagta gcccgttgca
     1321 accgagcgtt gatacctcaa ttcccacact cccgtcggca tcgccttcag atcagccacc
     1381 ggcaacattc aaacaacaga gcagctcaac cacgggatcg tccttcaggc ccgccgacag
     1441 cacaagcggg ggaatgaatg ccacgggcac aagtgtcccg gtgaacgaaa caaaacccct
     1501 ggtgcggcag gccctttacg aaaagccgat cccactcgtc gaggaggtgg ccgacccgcc
     1561 gatggttgat gtcactggct ggcggcggag tcagtgacaa gtgatctgat tcccgcgggt
     1621 actcaccgtt tgggatagat cgttatggat cgcccgacgc ggcgaaaagt tagtccgtgg
     1681 ggatcgagaa ctgcccgcca aagttcgtca atcgtcccat tctggacctg aaagcggatg
     1741 ggttgatcca gtcgaatccc tgcctctatg gcagactggc gattgaaatc gacctgcatc
     1801 tccaattgtt gcgcaagctg gcggatgacg ccttccagcg tgcctcggcc ttcccgcacg
     1861 gtaaatcggc gctgctcaaa tggatcacgg gcctcggggg ttgtcggcgg tggaccatcg
     1921 gagccgcgcg gggctgtcgg gcctttttgg gttctcaaaa gatcggccag cttgtccagc
     1981 gtttcgcgtg gtccctcgac aacaacatcc tggccaaacg ttgtcacttt gcaatcggga
     2041 aacattttct gccattgctg ggccaatatc cgcgcttgag gtccgacgga aaactgtctc
     2101 gccacggcca ttgtgttggt aatgggcaca atccgtattt cgcccgattc attgagttca
     2161 aacgtccggt cgaactgtgc caatacaagc gtcaaacggt caacgagcgt caaaggggga
     2221 agcgatccct cgggccagag gtcgtgaaga atctcatcgg gattcgtcaa tcgccaattg
     2281 gcctcttgca accagagggt aacgagttgg cgaggcgtgg tcaatcttgg ccaagagacg
     2341 gccagcgggc gcagtccggc aagccccacc ttgccaagct tcagggcctc ttgccgtcgc
     2401 gcgaccatga gcgattccaa gcgttggact gcctctggcg gacccacgta cacgatcggg
     2461 gaaacgacaa cgctttgaac tccgcacgcc tcggcaatgg ccgcgatgac ctcccgcagg
     2521 ggtcgatccc gcacagtcaa cgtcagctct tggtcaggat cacaccttcg atccaggacg
     2581 atggcgactt gatgcgttcg tcccaggcgg agaaggccct cccgcaaagg cacatcggtc
     2641 cagaagatac ccgttggagt gtcgagccgc cgcaagaatt cgttgccgat gacaaaggat
     2701 gggccttctt ggcaccaggc ggggcttatc cccacgcaaa cgagcgccag gacgggccct
     2761 atcaataagg cttgcgatcg aatgactgga cgtctcatcg cgtaatattc aaatcacaat
     2821 gccacgggga gcacgccaac ttggcccaaa ggccgcctct gccaaaaggc aacgaggctc
     2881 catgatttga cgtgttcacc gtatcagcca cggccattcc tttcttgtag tttagttaga
     2941 gcctatccca taatctcctc ttacccgttc ggcgggaaag gggttggggg tgagggtggc
     3001 tcgaatcgct gtcggttttc tcaatcgact ccgacgatcg cttgccctcg ccccaaccct
     3061 ctcccggagg gagaggggga cttttcggga tagactctta acctgccgcg gtgaggcacg
     3121 acttggccgt ttaacctgcc aggatgatca tttcaccttt ccaccgtact gtcccacgtg
     3181 ttcatcaccg ggtacatggc gccgtgtcgt tcccccatag cggctatagg agatgatcct
     3241 gcggtggcga atcagcctgg gccatcgccc tgggggtatc aggggtgccg tggttatttg
     3301 gtggggcaag gggccactcc tgaccgatcc aaccgcggcg ataaaagctc cagatcatca
     3361 caccggctaa agccagcatc aaagcgaggg caaaaggata tcccaaatac caacgcagtt
     3421 ccggcatgtt ccagggggaa acatcgggat caaaattcat accatagacc ccggc
//
LOCUS       DAOSUT010000268         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11157_length_3459_cov_8.538778, whole genome shotgun sequence.
ACCESSION   DAOSUT010000268 DAOSUT010000000
VERSION     DAOSUT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_3459_cov_8.538778"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..932
                     /locus_tag="PLD05_15435"
     CDS             114..932
                     /locus_tag="PLD05_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78877.1"
                     /translation="MVKKLLIGGGIVVLLGTVLLGRDLVSYVRTSANYVQESVVDAVP
                     MEFQIRRARKMLDDLLPEIRKNMHVIAREEVEVERLERQIAQAREQLEKEKQVILRMK
                     DDLAGGRPTLVYSGREYTAQQVRTDLANRFERFKTNEATLRNLEQIREARLKSLQAAR
                     DKMEGMLAAKRQLEVEVENLQARLQMVQAAQTTSEYQIDDSTLGRVKDLIADLKAKLD
                     VAERLVQSQGFYQGEIPVEDPNQKDIVDEVTQYFEASSADGEGNAEDAAITQAY"
     gene            949..1623
                     /locus_tag="PLD05_15440"
     CDS             949..1623
                     /locus_tag="PLD05_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOP78878.1"
                     /translation="MQPNACPGMTSDDACEVAMAGPPREGHSMRRFLLWIDGVGGYLV
                     CLGETIRIGRAGAGAVDVPLVADVAQHHAEIRRDGEGYFLESYAPTWLDGNPVRQAVM
                     HDRCRLQLGKHVILRFVRSTPLSLSARLDFESGHRTHPTTDGVLLMAHNLILGPGPAA
                     HVCCRDWRHNVILFRERDEVWCRAGRPFRVDGVPQERAARLTWSSLVEGEDFALRLEE
                     IPPSEV"
     gene            1741..>3459
                     /locus_tag="PLD05_15445"
     CDS             1741..>3459
                     /locus_tag="PLD05_15445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOP78879.1"
                     /translation="MNTVIAVVEKPKLNNIPEETHASPRGDSPSENVSRFVYASGSRP
                     LSGYTIKRGIGRGGFGEVYYAVSDSGKDVALKLLCRNWDVELRGINHCLNLKHPNLLT
                     IYDVRRDDRGDTWVIMEYIRGPSLEQVLADHPQGLPLDTALVWFRGMAAAVAYLHEHG
                     IVHRDLKPGNIFWEEGVVKVGDYGLAKFISCSRRSGQTESVGTVHYMAPEIAHGRYGK
                     EIDIYAMGVMLYEMLTGHVPFEGESVAEVLMKHLTKLPDLTPLPPEVRRVVGRALEKD
                     PARRFQDVREMVAFLPGMEQSPYVEMGTAKVASGSTEEEKEQFQSPGSAKMGHGEEPV
                     MAELAEESNRPEPIWHAVTQAFRDLRTAWNNSRLATPLRVAILIGVAFLVISHVGILG
                     PVIFGLLMAYAVYWTVRAILIATMGQEHVVRGTAIPAELVAAPAQPTWPQRVAAGAPP
                     PVPEPSRAEAFRQYMSQMSFRAHLAETLGSFLAAFLIAGVLAVVGYLVQSFYGKTMTL
                     TQASWVFLITILGSWTILLVSKVWQVWESDASVRRFVMMVLGLALGIVGFGLTQYLQV
                     DLTFDAR"
BASE COUNT          722 a          847 c         1106 g          784 t
ORIGIN      
        1 gcttgtgcga atatacggat agcaggagct gaaaagctcg cttgttggtg ctgtcgattg
       61 caggtgtgtt aagccctggc ctcagggtca cgagaaaaag gagggtcacg atcatggtga
      121 agaagctgct tatcggtggt ggtattgtcg ttctgttggg gaccgtttta ctcggtcggg
      181 acttggtgag ctacgttcgt acctcggcca actatgtcca ggagagtgtt gtcgatgcgg
      241 tgcccatgga gtttcaaatt cgtcgtgccc gcaagatgct tgacgatctt ctgcccgaaa
      301 ttcgcaagaa catgcatgtc attgcccggg aagaggtgga agtggaacgg ctggagcggc
      361 aaattgccca ggcccgtgag caattggaaa aggaaaagca agtgatcctg cggatgaagg
      421 atgatctcgc cggtgggagg cccacactgg tgtattccgg acgggagtac actgcccagc
      481 aggtgcggac cgatctggcc aatcgttttg aacgattcaa aaccaacgaa gcgacgctga
      541 gaaatctgga acagattcgc gaggcaagac tgaagagtct ccaggcggct cgggataaaa
      601 tggaagggat gctggccgcc aaacgacagc tcgaagtgga ggtggagaat cttcaggccc
      661 gattgcaaat ggtccaggcg gctcagacga caagtgagta tcaaattgat gatagcaccc
      721 tggggcgagt caaagacttg atcgctgacc tcaaggcgaa attggatgtc gcggagcggt
      781 tagtccagtc gcagggattc tatcaaggag aaattcccgt tgaggatccc aaccaaaaag
      841 acattgtgga tgaggtgacg cagtacttcg aggccagctc cgcggacggg gaaggtaacg
      901 ccgaggatgc agcgatcacg caggcctatt gatttcggag gtgcgatcat gcagccgaat
      961 gcctgccccg ggatgacatc ggatgatgcc tgtgaggtcg caatggctgg tccaccgcgg
     1021 gaaggtcatt ccatgcgacg gtttcttctc tggatcgacg gcgtgggagg gtacctggtc
     1081 tgtctgggag agaccatccg gataggccgg gctggtgcgg gggccgtgga tgtccccctg
     1141 gtcgccgacg tagcgcagca tcatgcggag attcgtcgcg acggagaggg ctattttctg
     1201 gagagctatg cgcccacgtg gttggatggc aaccccgtac ggcaggccgt gatgcacgat
     1261 cgttgtcgct tacaactggg aaaacatgtc atcctacgct ttgttcgcag cacaccgttg
     1321 agtctttcgg cccggttgga tttcgaaagt ggtcatagga cgcatcctac tacggacggc
     1381 gtattgctca tggcccacaa tctgatcctg gggcctggcc ccgcggccca tgtttgttgc
     1441 cgagattggc ggcacaatgt cattctcttt cgagaacggg atgaggtgtg gtgtcgggcc
     1501 ggaagaccgt ttcgtgtgga tggtgttcct caagagaggg ccgcacgatt gacatggtca
     1561 tcattggttg agggagaaga cttcgccctc cgtttggagg agattcctcc ttcagaagta
     1621 tgatgcggaa gaccgggtga ttcactctca cggacgcaca taacgggtgt gtccgttaga
     1681 atgaatgatg accgtcgcac tgatccaagg tcggtgccag taagtcagga gagtcctcta
     1741 gtgaatacgg tgatagccgt ggttgagaaa cctaaactca acaacatccc tgaagagacg
     1801 catgcttcac cacgggggga ctcaccctcg gagaacgtga gccgtttcgt ttacgccagc
     1861 ggttcgcggc ccctttccgg ctacacgatc aagcggggga tcgggcgagg agggttcggg
     1921 gaagtctatt atgccgtcag cgactccggg aaggacgtgg cgctcaagct cctttgtcga
     1981 aattgggatg tggaactccg cggaatcaac cactgtttga acctcaaaca cccgaacctc
     2041 ctgacgatct acgatgtgcg acgggatgat cgcggggaca cctgggtcat tatggagtat
     2101 attcggggac cgtcgctgga acaggttctc gccgatcatc cccaaggcct tccgcttgac
     2161 acggctctgg tttggttccg tggaatggcc gctgccgtgg cttatttgca cgaacatggg
     2221 attgtccatc gcgatttgaa gccgggcaat atcttttggg aagagggcgt ggtcaaagta
     2281 ggggattatg gcctggccaa gttcatttct tgcagtcgcc gcagtggaca gacagaaagt
     2341 gtgggtactg tccattacat ggcgcctgag atcgcccatg gccgttatgg caaagagatc
     2401 gacatctacg cgatgggggt catgctttac gagatgctga ccggccatgt tccgtttgag
     2461 ggagagagtg tcgccgaagt tttgatgaaa catctgacga agttgccgga cctcacgccg
     2521 cttccgccgg aagtccgtcg cgtggtcggc cgggcactgg aaaaggaccc cgcacgtcga
     2581 tttcaggatg tccgagagat ggtggctttt ttgccaggaa tggaacagtc gccttacgtt
     2641 gagatgggta ctgcgaaagt ggcaagtggc tcgaccgagg aggagaagga gcagttccag
     2701 tcgcctggct cagccaagat gggtcacgga gaagaaccgg tcatggccga actggcggag
     2761 gaatcgaatc gtcccgaacc tatttggcac gcggtgactc aagcctttcg ggatttgcgt
     2821 acggcctgga ataattcgcg cttggctacg ccactaaggg tggcaattct catcggtgtc
     2881 gctttcctgg taatctccca tgtgggaatc ctcgggccgg tcattttcgg gcttttgatg
     2941 gcttatgcgg tgtattggac cgttcgggct atcctgattg cgactatggg ccaggagcat
     3001 gtggttcgtg gtacggctat tcctgcggaa ttggtcgctg ccccggcgca accgacgtgg
     3061 ccccagcgag ttgctgccgg tgcgccgcca cccgttcccg agccttctcg ggctgaggcg
     3121 ttccgtcagt atatgagcca aatgtccttc cgtgcccacc ttgcggaaac actggggtcg
     3181 tttctcgcgg cctttctcat cgcgggtgtc ctagctgtcg tcggttacct ggtgcagagt
     3241 ttctatggga agacgatgac gctgacacag gcatcctggg tttttttgat cacgatcctg
     3301 ggaagctgga ccatcttgct ggtcagcaaa gtgtggcaag tgtgggaaag cgatgccagc
     3361 gtacggcgtt ttgtgatgat ggtcctcggt ttggcgctgg ggattgtggg ctttggcttg
     3421 acgcaatacc tccaggtgga cttaacattt gacgcccgc
//
LOCUS       DAOSUT010000269         3397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11404_length_3397_cov_8.517654, whole genome shotgun sequence.
ACCESSION   DAOSUT010000269 DAOSUT010000000
VERSION     DAOSUT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11404_length_3397_cov_8.517654"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..843
                     /gene="pncA"
                     /locus_tag="PLD05_15450"
     CDS             220..843
                     /gene="pncA"
                     /locus_tag="PLD05_15450"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOP78880.1"
                     /translation="MDALIVVDVQNDFCPGGALPVPDGDLVVPVINRLMESFPLVVAT
                     QDWHPANHKSFAANHPGRQPGDVIELAGVLQILWPIHCVQGTEGAEFHPDLNRAGIHV
                     VFQKGTDPEVDSYSGFFDNARKHDTGLHEFLQRRGVARLFVCGLATDYCVKFTCLDGR
                     SLGYEVFLVLDASRGVELHTGDVAKAIDEMKAAGVIPIESTEIAMVR"
     gene            complement(879..>3397)
                     /locus_tag="PLD05_15455"
     CDS             complement(879..>3397)
                     /locus_tag="PLD05_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78881.1"
                     /translation="GNIILRGQGPRQDFRVTTRDVTLTEHRLWTNHPVEFLWGRHRAR
                     GSGLEIVFEGRSPFSASPHDASPFRIQRFLLRHVDRLHIDISALQATATPPADSKSSS
                     GPSEVQDSFLEIACQGPLLCHPLQRIVTFEDRVDVTRVQGSQTILRLTCDRLSLLLGD
                     KPTPTEQSSKSAPTVPTSQRSASPVALKLIQAVGRPVVASSPVWKGEISCEDLRFDVE
                     QGLLSMEGPAGVTVKQATNQLQAPRVQCQVDSSGKIRTIAAAGPGEARGQIPDATPAD
                     AVVSWETGFFWKFEEPERLMALQGNVRIVAAAIGRLQCGQLWCWLEPTTDGPLASSQL
                     RPRRIVAQDRVQVESPQIQANTERLESWFVWPASESPNSKADRAAEGFSLGSANNQKA
                     TDKDQSPSQLKVTARLLRFEFQITGNRGELSALSADGTVKVQEKPTQPDAQPLEVYGE
                     HLEIIGASTPQTVFALTGNPAFVAGRGLSLSGFQIFLDRGRNLVSTSGPGRVEINQGS
                     QKADLLLPVSTERVEIQWSQGMDFDGQTVTFKGNVQLTSARGRIHAEILEAQISPSIV
                     LSDLKTTQPIQLYAARAVGNVLIENVDVNTQGEVQSRDRLYVSEIAMNWETGAITAPG
                     FGRFLTVRSASLVPRNPGHSLRSAPSQGSELARGTTSADGGSPLQPSQPVALEITYSQ
                     GLAGNVHQRTISCRGRVKAKYGTVDRWDVAQLPDDPAGLGPQGLMLHSDEIAVYQMVT
                     PGMTEPHLELEARGNVTVEGTSHTARARRLAFDESKTLLTLEGDGDVPAELYRQEFTG
                     GPVHKTAARRIYYWYNTKTIKVDDPKSLEVSQSPSPYSNR"
BASE COUNT          717 a          890 c         1025 g          765 t
ORIGIN      
        1 gcaagagaaa cctgcgggaa agcaagcgaa gggtggcttg ttccagttcg atgaggccat
       61 ctatcaagaa ttgaccaaga ccggattttc gccgttcctt tgtgggaaga gatgtgactg
      121 aatatggaga tgggagacga atgcgggatt acgcgaatta gccgacgctc gcgttcgctt
      181 agcaggggcg gccgatcata ttaagcgagg gttaagacaa tggatgccct gatcgtcgtt
      241 gacgttcaga acgacttctg ccctggaggc gctttaccgg tgcccgatgg tgatttagtt
      301 gttccggtga tcaaccgcct tatggagagc ttcccattgg ttgtcgccac ccaggactgg
      361 catcccgcga atcacaagag ctttgcggct aatcatccgg gacggcagcc gggagacgtg
      421 atcgagcttg ccggggttct ccaaattctt tggcctatac attgcgtcca agggacagaa
      481 ggggctgagt ttcatcccga tttgaatcgg gcaggcatac acgttgtctt ccaaaaagga
      541 acggacccgg aagtggacag ttacagtggt ttttttgaca atgcccgcaa gcatgataca
      601 ggtttgcacg aatttctgca gcgtcggggt gtcgctcgtc tttttgtctg tggcttagcc
      661 accgactatt gtgtgaaatt cacgtgcttg gatggcagat cgctgggtta tgaggtcttt
      721 ttggtgctgg atgcatcaag aggtgttgag cttcacactg gagatgtggc caaggcgatc
      781 gacgagatga aagccgccgg cgtaatacct attgagagta ccgaaattgc tatggtccgg
      841 tgagtcttac tgagtgggcc ttggcactaa gccgatggtt agcgattgga atagggcgag
      901 ggagactgac ttacctccag cgatttgggg tcatccacct tgattgtttt ggtgttgtac
      961 cagtagtaaa ttcgccgggc agcggtctta tgaacagggc ccccagtaaa ctcctggcgg
     1021 tacaattcgg ccgggacatc gccatcacct tccagtgtga gaagtgtctt cgattcatca
     1081 aaggcaaggc gacgggcacg agccgtgtgc gatgtcccct cgacggttac gttacccctg
     1141 gcttctagtt ccaaatgggg ctccgtcatc cccggggtga ccatctgata gacggcaatt
     1201 tcgtcactgt gcagcatgag cccttgtggt cccagcccgg cgggatcgtc gggtaattga
     1261 gcgacgtccc agcgatcgac ggtgccatat tttgccttaa cgcggccccg acaagatatc
     1321 gtccgctggt gcacattgcc ggcgagcccc tgggagtagg tgatttccaa agccaccggc
     1381 tgagacggct gtagggggga accaccatcg gcggacgtag ttccacgtgc cagttcgctc
     1441 ccttggcttg gcgcagaccg caaggaatgg ccggggttcc gtggcacaag gctagcagag
     1501 cgaaccgtga gaaaacgtcc gaagccaggc gccgtgatgg cgccggtctc ccaattcatc
     1561 gcgatttcgg agacgtataa acgatctcga gattggactt ctccctgagt attgacgtcc
     1621 acattttcga tcagaacatt tccaacagcc ctcgctgcgt aaagttgaat cggttgcgtg
     1681 gtctttaagt cggagaggac aatcgacgga cttatttgag cttccaggat ctccgcgtgg
     1741 atacggccac gggcgctggt caactggaca ttacctttga aggtgacggt ctggccgtcg
     1801 aagtccattc cttgcgacca ttgaatttcc accctctcag tcgaaaccgg cagaagcaga
     1861 tcggcctttt ggctgccttg attgatttcg acgcgtccgg gacctgaggt cgagaccaag
     1921 tttcggccac ggtcgagaaa gatttgaaag ccactaaggg atagacctcg cccggccaca
     1981 aaggcgggat tacccgttag ggcaaacacc gtttgaggag tagaggctcc gatgatctca
     2041 agatgctcgc cgtacacttc caggggttgg gcgtcgggtt gtgttggttt ctcttgaacc
     2101 ttcactgtcc catctgcgga aagggccgac aattctccgc gatttccggt aatttggaat
     2161 tcaaaccgca agagtcgtgc cgtgaccttt aactgggaag gactttgatc cttgtcggtt
     2221 gccttctgat tgttcgcgct ccccaaagag aaaccctctg cggcgcgatc ggccttgcta
     2281 tttggggatt cggacgccgg ccaaacgaac caagattcca gccgttcggt gttcgcttga
     2341 atttgggggg actcgacctg aacccgatcc tgagcgacga tgcgccgcgg tcgtagttgg
     2401 gaagaagcca aaggaccgtc ggtggtgggt tctaaccagc accacaattg gccacattgc
     2461 agccggccga tagcggcagc gacaattcgc acgttgccct gaagggccat caagcgctct
     2521 ggttcttcga atttccagaa aaagccggtt tcccaggaga ccaccgcgtc cgcaggtgtc
     2581 gcgtccggga tttgaccacg tgcttcgccg ggaccggcgg ccgcaatggt ccggattttg
     2641 ccgctcgaat cgacctgaca ttgaacccgc ggggcctgaa gctgattcgt cgcctgtttg
     2701 acggtaacgc ctgcgggtcc ttccatcgac aggagcccct gttcaacatc aaagcgaagg
     2761 tcttcgcagg agatctcacc cttccagacg ggcgacgacg caacgacagg acgccctacg
     2821 gcttgaatga gtttcaaagc caccggcgat gctgaacgtt gggatgttgg tactgttggg
     2881 gcactcttgc tactttgctc cgtcggagtc ggtttgtcgc cgagtaagag gctcaaccga
     2941 tcgcacgtta gccgcaagat ggtttggctg ccctggaccc gagtcacgtc aacacgatcc
     3001 tcaaaggtga ctatcctttg cagcggatgg cagagcaggg ggccttggca ggcgatttcc
     3061 aagaagctgt cctggacttc ggaggggccg ctcgatgatt tcgaatccgc tggcggagtt
     3121 gcggttgctt gcaacgcgga aatgtcgatg tgcaaccgat cgacgtggcg gagcaggaat
     3181 cgctgaatgc gaaagggcga ggcgtcatga ggactggccg aaaatgggga tcttccctcg
     3241 aagacgattt ccagccccga accacgggcg cggtgacgtc cccataaaaa ctccacggga
     3301 tgattggtcc agaggcggtg ctccgtaagc gtgacatccc gggtggtcac gcggaagtcc
     3361 tgacgaggcc cttggccgcg gaggatgata ttgccga
//
LOCUS       DAOSUT010000270         3385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11459_length_3385_cov_10.708108, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000270 DAOSUT010000000
VERSION     DAOSUT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11459_length_3385_cov_10.708108"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2174)
                     /locus_tag="PLD05_15460"
     CDS             complement(150..2174)
                     /locus_tag="PLD05_15460"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOP78882.1"
                     /translation="MKSSVSRGVRWASVAWLLVASSVFSQETASMHFRRGEMMVAARR
                     FQLRETQTNLPAIEPGTELVLVMRESDKLAVWVEIPPEDGLFRFPRWVNGWINQKDAV
                     PGRGGKHWWAHLLSDEQRNRLTQLAVEGLQSRKYGVPTAAASLLGHLNAQQAARPLWD
                     ILEVKEHSLAWQAAQSLGKLGPEGQALLREALQHEKPEVRRLAAKGLGFYGDEESFPA
                     LRAALQDTDPRVREAVAQSLFRLGDETTVAAVWQKGESVSFRIREENLLDATLCELAA
                     GGLAKRGDAGIALLREGFKHSHPFVVRSVIITVGKFGYREFIPELRKAMESPDQSGMA
                     VASLYVLGDEQVIPKIRVELGDSDPNRVAVAIERLNRAGDRESVPKIRRLLENRWPQV
                     RSAAARALGSFEDGDSIPRLRAALHDEAGSVRAAAAWALGRLGDLEALPDLKRILASD
                     PFDGARQSAAEAMTMFGEAGKNAVAEGLRSDSASTRWAVASALRYFDPDKGLALLSSA
                     VADPVPRVRQAAVESLGRISKEEAIPLLAEACYDVSEGVSAAALHELLAQGWRPSSSM
                     EDLLHVVVATEDVPVLQMLTADSKPVLLALLSAEGLRHKKAAKMLIRLGDKETIPDLI
                     AYLNRQGDEIVTEWFANSQNAELSAAARKHAKARGYIVRTISPPKVVWGR"
     gene            2504..>3385
                     /locus_tag="PLD05_15465"
     CDS             2504..>3385
                     /locus_tag="PLD05_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78883.1"
                     /translation="MNVILAATLLTNCLLAVGEAPSLPDTSKMNILLIDIEDCNAAAL
                     GCYGNPICKTPHLDRFVQTAVRFDRAYVQAVCCNPSRTSFLTGYRPLTTRVTSNGQEM
                     DAHLPEGMLTLPEYLKTRGLYLAVIGKLFHRTDYAEKQLSVFDRIEMYTPPPGWKGPG
                     PVIKFPPVARPAGVPKPPADEKSPEYREWRRQQSDRYGDSGRAPEEEGDYRMAATAVA
                     LLKEFAQTKQQFFLAVAQSKPHTPLLAPKKYIDMYNPADIPDPPAPAQSLVNFPYPKR
                     AFGGNPDIFTLKQPTKQQ"
BASE COUNT          741 a         1057 c          907 g          680 t
ORIGIN      
        1 gttttacttt gtagtaacat accccatggg ccggttcaac cccttcggag gcccggcttt
       61 tcttgcatgc aatgctgcta aggaccgttg agagagtgat cccacggccc gggcaggaca
      121 atctgatcgc gatcgaagat gcggatgcct caccgccccc aaacgacttt cggcggtgag
      181 atcgtcctga cgatgtagcc gcgggccttg gcgtgttttc gagcggcagc agacagctcg
      241 gcgttttgag aatttgcgaa ccactcggtc actatctcgt cgccctgccg attcaaatat
      301 gcgatcagat ccggaatcgt ctccttgtca cccaaccgaa tgagcatttt cgcggccttt
      361 ttgtggcgca gtccttcagc ggacagcaag gcgagaagca ccggcttgct gtcggcagtg
      421 agcatttgca ggaccggaac gtcttctgtc gccacgacca catgtaggag gtcttccata
      481 gacgaacttg gccgccaacc ctgggccaaa agttcatgga gggccgccgc tgagacgcct
      541 tcgctcacgt cgtaacaggc ctccgccaag agaggaatgg cttcctcctt gctgatgcgc
      601 cctaacgact cgacggcagc ttgtcgcacg cgcggcacgg ggtccgccac tgctgacgaa
      661 agcaacgcca gtcctttgtc agggtcgaaa taccgtagcg cggaagcgac agcccaccgc
      721 gttgaagcgc tgtccgagcg caacccctcg gcgacggcgt tcttgcccgc ctcgccgaac
      781 atggtcatcg cctccgccgc tgactgtcga gcaccgtcaa atggatcgct ggcaagaatc
      841 cgcttgagat caggcaacgc ctccaaatcg ccaagcctac ctaaggccca ggccgcggca
      901 gcgcggacgc tgcccgcctc atcatgcaat gctgctcgta atctgggaat gctgtcgcca
      961 tcttcgaaac tccctaacgc ccttgccgca gccgaccgca cctgaggcca tcgattttca
     1021 agtaaccggc ggattttggg aaccgattca cgatcaccgg cacggttcaa tcgctcaatg
     1081 gcgacagcca cccgattggg gtcggaatcc cctaactcaa cacgaatctt aggaatcacc
     1141 tgctcgtcac cgagaacgta caaagacgcc actgccatcc ccgattggtc cggactctcc
     1201 atcgcctttc gtagttcagg gatgaactct cggtagccga atttcccaac cgtgattatt
     1261 accgagcgaa ccacgaaggg gtgggagtgc ttgaaccctt cccggagcaa ggcgatcccg
     1321 gcatcgccac gtttggccag tccacccgct gccaactcac agagtgtggc atccaagaga
     1381 ttttcttccc taatccgaaa gctcacggat tctccctttt gccacacggc ggccacagtc
     1441 gtctcgtctc ccaaacggaa aagtgactgc gccactgcct cccgcactcg cgggtctgta
     1501 tcttgtagcg ccgcgcgaag ggcaggaaaa gattcctcat cgccgtaaaa accgagaccc
     1561 ttggcagcca accggcgcac ttccggcttt tcgtgctgca aagcctcgcg gagaagggcc
     1621 tggccttccg ggcccagctt gcccaacgac tgggccgctt gccaagctaa cgagtgctct
     1681 tttacttcga gtatatccca cagaggccgg gccgcttgct gagcgttcaa gtgccccaaa
     1741 agggaagccg cagcggtcgg cacgccgtac tttcttgact ggagtccctc cacggccagc
     1801 tgcgttaatc gattgcgctg ctcatcggac agaagatgcg cccaccagtg ttttccccca
     1861 cgaccgggga cggcgtcttt ctgattgatc caaccgttga cccatcgggg gaagcgaaag
     1921 agaccatctt ccgggggtat ctctacccaa accgccaatt tgtcggattc ccgcatgaca
     1981 agcacgagct cggttccggg ctcaattgcc gggaggtttg tctgggtctc acgaagctgg
     2041 aaacgcctcg cggcaaccat catttcgccg cgtcgaaagt gcatgctcgc cgtttcctga
     2101 ctgaagaccg acgaggcaac aagcagccac gccacggacg cccaccgtac accacgggag
     2161 acagaagatt tcataggctc gtcctctcgg atattctgcg gatcaccccg gcacgccgcc
     2221 aatcatatca tagggcatga tcacctaaag aagcaacaag ccaaacgaaa agagacgggt
     2281 tgcgaaacat gaaaaaccgt taaccctcga ttcggaaccc atgggtgcga tccagagagt
     2341 gccctgcgga caagggtccg gtatatcctg caagccgaaa gtaattcctc tataatggtt
     2401 gcgagcatgc cgcgaaaacg gcatgcggtc tagtgccact tcggcgcaga agccgtgtgg
     2461 tttgtcggtt taggtggttt tcctgatttg gggaggtcct accatgaatg tcattttggc
     2521 cgcaacgcta ttgactaact gcctgttggc cgtcggcgag gcgccatcgc tgcccgacac
     2581 atcgaagatg aacattttgc tcatcgatat cgaggattgc aatgccgcgg cgttggggtg
     2641 ctatggtaat cccatctgca aaacgccgca tctggatcgg ttcgtccaaa cggctgtgcg
     2701 gttcgatcga gcttacgtcc aggcggtctg ctgcaatccg tcacggacat cgtttctcac
     2761 aggttatcgt ccgttgacca ctcgtgtaac aagcaatggc caggaaatgg acgcccatct
     2821 gcccgaagga atgctcacat tgcccgagta tctgaaaact cgcggccttt acttggccgt
     2881 cattggtaag ttattccatc gcacggatta tgcagaaaag caactttcgg tgttcgaccg
     2941 catcgagatg tacacacctc cgccgggatg gaaaggcccc ggtccggtga tcaagttccc
     3001 acccgtagcg agacccgccg gcgttcccaa gcccccagcc gatgaaaaga gcccagaata
     3061 ccgcgagtgg cgacgccagc agtccgaccg ctacggcgac tcgggacgag cgcccgaaga
     3121 ggagggggat taccgtatgg cggccacggc cgtagcgctg cttaaggagt tcgcgcaaac
     3181 aaagcagcaa ttcttcttgg cggtagccca gtcgaagccc catacgccgt tgcttgcgcc
     3241 caaaaagtat atcgacatgt acaatcctgc cgatatcccc gatcctccgg ctcctgcgca
     3301 aagcctggtg aatttcccct atcccaagcg ggcatttggc ggcaatcccg acatcttcac
     3361 cctaaaacag ccgaccaaac agcaa
//
LOCUS       DAOSUT010000271         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11499_length_3378_cov_8.424917, whole genome shotgun sequence.
ACCESSION   DAOSUT010000271 DAOSUT010000000
VERSION     DAOSUT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11499_length_3378_cov_8.424917"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1757
                     /locus_tag="PLD05_15470"
     CDS             96..1757
                     /locus_tag="PLD05_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOP78884.1"
                     /translation="MAYQLPTLPKPPKAKRKQVYLVASGDLRLSANQVCWPAQKAMED
                     ALADAVREAGYELVRAHPYKEAEGHGFISSQKEGMEVFANIDPKARLIVAEAVWQYSH
                     HVLAGLLSHQGPILTVANWSGQWPGLVGMLNLNACLTKAGKPYCTLWSEDFSAPEFRR
                     KLATWLEKGEIRHPLKHVTPLKKVKIPGSIKRLGQALADELRWKKAIMGIFDEGCMGM
                     YNAIIPDELLMPLGVFKERLSQSTLYYETTQVSDVEAREVYQWMLDRGMKFNLGSNPE
                     TDLTEDQVLTQCKMYIAALRLADDFGCHTIGIQYQQGLKDLLPASDLVEGMLNNADRP
                     PVKSRDGKRILYEGEPLPHFNEVDECAGLDALMTYRIHKAMGQPVETTLHDVRWGDWD
                     RSGTVPDYVWVFLISGSAPPAHFIGGWAGASSERQPAMYFRLGGGTLKGISKPGEIVW
                     SRVYVDNGRLCMDIGRAGVVELPPEETERRWKETTPQWPIMHAVTYGVTRDQFMAKHK
                     ANHIQVAYAEDADAADAAALAKAAMADALGIRVNMCGTRKGGKPW"
     gene            1819..3378
                     /locus_tag="PLD05_15475"
     CDS             1819..3378
                     /locus_tag="PLD05_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOP78885.1"
                     /translation="MSEALQCVKKTAVITGWDFSTGGVKCLAFDIEGHVLAEVRLPTD
                     IWFGDSPATGIRELNVMQLEGQAYASTRAIAAELTRLGRIKDWVAGGISATHHTSARI
                     DRLGNQVRRAICWNDATLAEYHAKGLARLGGQERVRELIGGPWAIRYGLSHLVKDEVH
                     LSPADWRRTYRIAPHGVLAAGYLTGNFDVCSVSVGASSGIMDLRTKKWRWEMLEAIEN
                     EEYRRLVKKQLPKIIDQNTPIGPLSESVALSAGLPPRVRPLIFPTSDDQQAGLVGGGA
                     VDAGQVAVILGNSAVVNSSSADRPQRGQLDVMRLNWGPYLWMRCYNNGAQFLDQVVGP
                     QPNWGELEQAARNELPGCDGTLVLPFVNPEPSLGISQKDAQLRWIPQMPETMGRRFRA
                     SLEAIAYLIALAVREHEDAGQKITRITVSGGIARSNLMCEILATVLGRSLVRLESEEG
                     PALGAAVTALAALESHLRKAKGIRKKFTVADAVAAMVRFRGTVEPNPAWAEAYRMGFE
                     RFCNELARIKE"
BASE COUNT          713 a          899 c         1070 g          696 t
ORIGIN      
        1 acaaatggct atcatgatac gccaaggctg ttttgtttct cttggagtat gaagccgagg
       61 catagccacc ttttcaaatg ggaaggagtc aagagatggc ctatcaatta cccacattgc
      121 ccaaaccacc taaagccaag cgaaagcagg tctatttggt agccagcggg gaccttcgcc
      181 tttcggcaaa ccaggtttgc tggccggcac agaaggccat ggaagacgcc ttggccgatg
      241 ccgttcgcga ggctgggtat gagctggtgc gggcacatcc ctataaggaa gcggaaggcc
      301 atggtttcat cagctcgcaa aaggaaggca tggaggtctt cgccaacatc gatcccaagg
      361 cgcggctgat cgtcgcggag gccgtgtggc agtattcgca tcatgtgctg gccgggctgc
      421 tcagtcacca ggggcctatt ttgacggtgg ccaattggtc ggggcaatgg ccgggcttgg
      481 tgggcatgct caacctcaac gcatgcttaa cgaaagctgg caagccgtac tgcacgcttt
      541 ggagcgaaga cttttctgcc ccagagtttc gccgaaagct ggcaacgtgg ttggagaaag
      601 gggagattcg gcatccgctc aagcacgtga ctccgctgaa aaaggtcaag attcccgggt
      661 ccatcaagcg gttggggcaa gccttggcgg atgaactccg ctggaaaaag gccatcatgg
      721 ggattttcga cgaaggttgc atgggcatgt acaacgccat catccccgat gaactattga
      781 tgccgttggg tgtgttcaaa gagaggctga gccaatccac actgtactac gagacgaccc
      841 aggtaagcga tgtcgaggca cgggaagtgt accagtggat gctcgaccgc ggcatgaagt
      901 ttaatctggg cagcaatccc gagacggatt tgacggaaga tcaagtcctc acgcaatgca
      961 agatgtacat cgcggccttg cgactcgcgg acgatttcgg ttgccacacc atcggcattc
     1021 aataccagca aggtttgaag gacctccttc ctgccagtga tttggtcgaa ggcatgttga
     1081 acaacgccga tcgcccgccg gtcaagagcc gggatggtaa acgcattctg tacgaaggcg
     1141 agcccttgcc gcactttaac gaagtggatg agtgcgcggg tctggacgct ttgatgacct
     1201 atcggattca caaggccatg ggccaaccgg tggaaaccac gctccacgat gttcgttggg
     1261 gcgactggga ccgctcgggc accgttcccg attatgtatg ggtcttcctt atcagtggct
     1321 cggcaccacc ggctcacttc attgggggct gggcgggagc atccagcgaa cgtcaaccgg
     1381 cgatgtattt tcgcctgggt ggcgggaccc tcaaaggaat ttccaagccg ggggaaatcg
     1441 tctggtctcg ggtgtacgtg gacaacggcc ggctgtgtat ggacatcggt cgggcaggcg
     1501 tggtggagct tcctccggaa gagaccgaac gccgctggaa agagaccaca ccccagtggc
     1561 cgatcatgca tgccgtcacc tatggcgtca cgcgggatca attcatggcc aagcataagg
     1621 ctaatcacat ccaggtagcg tatgccgagg atgccgatgc cgcggacgca gcggctctgg
     1681 ccaaggcggc gatggccgat gccttgggaa tccgcgtcaa tatgtgtgga acacgcaaag
     1741 gtggaaaacc ttggtgactt gccgtcatct ttgctggtga gattccgggc cgagatcgcc
     1801 gtataggagg ccaatgtgat gtccgaagcg ctacagtgtg tcaaaaagac ggccgttatt
     1861 acaggctggg attttagcac cggtggtgtg aagtgtttgg ccttcgacat tgaaggccac
     1921 gtcttggcag aggtgcggtt gccgacggat atttggttcg gggatagtcc ggccacgggc
     1981 atccgcgagt taaacgtgat gcaattggaa ggtcaggcgt atgcatccac gcgggccatc
     2041 gcagcggagt tgacgcgact gggacggatc aaggattggg tcgccggggg gatttcggct
     2101 acccaccata cgtcagcccg catcgaccgc ttgggcaatc aagttcgccg ggcaatctgc
     2161 tggaacgacg ccacccttgc agaatatcac gcgaaagggc tggctcgact cggtggccag
     2221 gagcgcgtcc gcgagttgat tggcgggcct tgggctatcc gttacgggtt aagtcacttg
     2281 gtcaaggatg aagttcacct tagcccggcc gattggcgcc gaacttaccg catcgccccg
     2341 cacggggtgc ttgcggcggg gtatcttacg ggcaattttg atgtgtgcag cgtctccgtc
     2401 ggggcctcct ccgggatcat ggacttgcgg acgaaaaagt ggcgctggga aatgctggag
     2461 gctatcgaaa acgaagagta tcggcgtctg gtcaagaaac aattgcccaa gatcatcgat
     2521 cagaacacgc cgatcgggcc gctctcggag agcgtggcgc tttcggccgg cttgccgccg
     2581 cgcgttcgac ccttgatttt ccctacctca gatgatcaac aggcagggct tgttggcggc
     2641 ggtgctgtcg atgcgggaca ggtggccgtc atcttgggaa actccgccgt tgtcaacagt
     2701 tcatcggccg atcggcccca gcgtgggcag ctcgatgtca tgcggctcaa ttgggggcct
     2761 tatctgtgga tgcggtgcta caacaatggc gcgcaatttt tggaccaggt ggttggtccc
     2821 cagccgaatt ggggtgaact ggaacaagcg gcccgcaatg aactgcctgg ttgcgatggc
     2881 accctcgttc tgccttttgt caatccggag ccgtcgcttg gcatctcgca gaaggatgcc
     2941 caactccgtt ggattccaca aatgcccgaa acaatgggac ggcgttttcg ggcatcgctg
     3001 gaggccattg cctatctaat cgccctcgcc gttcgggaac acgaagacgc tgggcaaaag
     3061 attacccgca ttacggtctc cggtgggatt gcccgaagca acctcatgtg tgagatcctc
     3121 gccacggttc tcggtcggtc gctagtgcga ctggagtcgg aagagggacc cgcgttgggg
     3181 gccgctgtga cggccttggc ggccctggaa tcccatctcc gcaaggcaaa gggaatccgg
     3241 aagaaattca ccgtcgccga cgcggtggcc gctatggtcc gcttccgcgg gacggtcgaa
     3301 cccaatccag cgtgggcgga ggcctatcgg atgggcttcg aacgcttttg taacgaactt
     3361 gcgcggatca aggagtaa
//
LOCUS       DAOSUT010000272         3336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11672_length_3336_cov_10.287717, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000272 DAOSUT010000000
VERSION     DAOSUT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11672_length_3336_cov_10.287717"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..>3336)
                     /locus_tag="PLD05_15480"
     CDS             complement(33..>3336)
                     /locus_tag="PLD05_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOP78886.1"
                     /translation="TWDWAMYRGWYKGQREFGSAVVPWDFCLAEWNAQFLGDRAYNIS
                     DQERRNLKWEAAQFRSGRLWYRWDYPHQLGSSDFDERYPIFAQYLTDNWRAFRTWGVS
                     AISPWEYHIYWKMRPGLARNQRTELSTDWENLQRPGYSPDYLEVRYERMDIAYEPSDW
                     VPTLAAQAMYRNNAPLLAYIAGGSTAFTAKDHNYLPGEVIEKQLIVINNSREPAEVKY
                     IWQFGTVPDHQAARIKGKGHLPVLIQEEIRPASVLLQGGDTLSVETGQQARAMVHLNL
                     PKDLSPGTYVLWAEFQFDNGDKQKDQFLVNILPLPDDQQLSAAVAQAAGRIGLFDPLG
                     ETRKLLDGLKVPYRVVSADSDLTGLEVLIIGKRAITADGPAPSLVRVREGLKVIVFEQ
                     TGEALEKRLGFRVAEYGLRNVFARVPNHPYLAGLNEDHLHDWRGEATLLPPRLKYEVS
                     DKYAGAPVVRWCGLEVTRLWRCGNRGNVASVLIEKPQVGDFLPILDGGFSLQYSPLLE
                     YREGRGVVLFCQMDVTGRTEGDPAAERLVRNILSYVLTTKYPVIPDRSAVYAGNTDGR
                     RYLEQAGIACDDYRPGVLGPEKVLIVTPGAGEILSPNAQQIAAWLAGGGRVVALGLDG
                     DEARRFLPIAVQTTPTEHIAAYFAPPSWTSQLAGISPADIHNRDPRILPLLTDGVDTA
                     LGGVLGESAQGCVIFCQMVPYQFVKRPRDVPSLRIIREDAAEGTQCAEVVMATVPYAQ
                     IGQKISAGSPGKLYTVAAMVKPVGSPATVRLEVERAGSPWDRAVRGKDQVLPPDRWTE
                     IYETFRVEKAYPEGWQAYVHIGQPEVCVRLDHFRLYEGDYVPTNSGDRKPQANFFTNP
                     GFESGSSSWYFNWNTEQQNLRKTFRRTAFLLSRLLANCGVRASTPLLERFGKPVQAAL
                     GPSLVKNGSFEIDEDQDSVPDGWLFSSENNLARCALQEVALPGVSRCVQISVPESGER
                     RGSAMLAQLNVPIHEGQWYRVSFWAKGENISSRQVTFAVQETTRWQAVLEYRRFTPEP
                     EWKEFTFLMPGRQTAETKTRLQIWFDGTGTLWLANFAITPCEPPSAGRWLEGLYLDVP
                     QEWDDPYRFFRW"
BASE COUNT          751 a          999 c          864 g          722 t
ORIGIN      
        1 accgttgttc gtcagcggcc gggcgaatct gatcaccacc ggaaaaaccg gtatgggtca
       61 tcccactcct gaggtacatc caggtacaag ccctctagcc agcggcccgc actaggtggt
      121 tcacagggcg taatcgcgaa attcgccagc cacaacgttc ccgtcccatc aaaccaaatc
      181 tgcaagcgcg ttttggtttc ggcagtctgt cggcccggca taaggaacgt gaattccttc
      241 cactccggct ccggcgtgaa ccgtcgatat tccaacacag cctgccagcg ggtcgtctct
      301 tggacggcaa aggtcacttg cctagaggag atattctcac ccttggccca aaaactcacg
      361 cgataccact gcccctcatg aatggggaca ttcaattgag ccaacatagc actgccccga
      421 cgttcaccgc tttccggaac gctaatctgc acacaccgcg acactccggg caacgccact
      481 tcctgtagag cacatcgggc tagattgttt tcactagaaa acagccatcc gtcgggcacg
      541 ctgtcttgat cttcgtcaat ttcgaagctt ccgttcttca ccaaagaagg ccctaatgct
      601 gcctgaaccg gtttgccaaa ccgctccaac agtggagtgc tcgcacggac gccacaattg
      661 gcaagtaacc ggctcaacaa gaatgccgtg cgacggaagg ttttgcggag attctgctgc
      721 tccgtattcc agttgaaata ccacgacgac gaaccagact caaatcccgg attggtgaag
      781 aaattagcct ggggtttcct gtcgcccgag ttcgttggga cataatcacc ttcgtagagg
      841 cggaaatgat ccaagcggac acagacctcc ggctggccga tgtgtacata agcctgccag
      901 ccttcgggat aggctttttc cacgcgaaat gtctcgtaaa tctcagtcca gcgatcaggt
      961 ggtagaactt ggtctttacc gcgcactgcg cggtcccagg gtgatcccgc tcgctcaact
     1021 tccaacctaa ccgtggcggg gctccctacc ggtttcacca tagcggcaac cgtgtagagc
     1081 ttgcccggtg agcctgccga aatcttctgt ccaatttggg cgtaggggac cgttgccatg
     1141 actacctcgg cacactgagt tccctccgcc gcgtcttcac ggatgatgcg gagagatggc
     1201 acatcccgag gacgctttac gaattgatag ggaaccatct gacagaagat gacacatccc
     1261 tgcgcggact ctcctaaaac accgccaaga gctgtatcca caccgtccgt caacaaaggc
     1321 aagatgcgag ggtcgcggtt atggatatcc gccggactga ttcccgctaa ttgagacgtc
     1381 catgagggcg gggcaaaata ggccgcaatg tgttctgtgg gggtcgtttg caccgctatg
     1441 ggcaagaagc gacgagcttc gtctccatcc aatcccagcg caaccaccct gccgccacca
     1501 gcgagccacg cggcaatctg ttgtgcatta ggtgataata tctccccggc cccgggcgta
     1561 acgatgagga ctttttccgg cccaaggact ccaggtcgat agtcatcgca agcgataccg
     1621 gcttgctcga gatatcggcg gccgtcggtg ttcccagcat acaccgccga acggtcagga
     1681 atcaccggat acttggtggt aagaacgtaa ctgagaatgt ttctcaccag acgttctgcg
     1741 gccgggtcac cttccgtccg tccggtcaca tccatctggc aaaaaaggac caccccccgc
     1801 ccctcgcggt actccaacaa cggactatat tggaggctga aaccgccatc gagaattggc
     1861 aaaaagtctc ccacttgggg cttttcaatg aggacggatg cgacatttcc tcgatttccg
     1921 caccgccaca aacgtgttac ctcaagacca caccaccgaa caacgggtgc gccggcgtac
     1981 ttgtcactga cctcgtactt gagccgaggt ggcaacagcg tggcctcacc ccgccagtca
     2041 tggagatggt cctcatttag tcctgccaga taaggatggt tagggacccg tgcaaagaca
     2101 tttcttagtc catattctgc cacccgaaag cccaatcgct tttccagggc ctcgccagtt
     2161 tgttcaaaga caatgacctt taggccctcc cgaacccgga cgagcgacgg cgcaggccca
     2221 tctgccgtga tcgcgcgttt gccaataatg aggacctcca ggccagtaag atcgctatcg
     2281 gctgagacta cccggtaggg taccttcaaa ccatctaaca gcttgcgtgt ctcgccgagg
     2341 ggatcaaaga gaccgattcg tcctgctgct tgggccacgg ctgcactaag ttgctgatcg
     2401 tccggcaagg gcaaaatgtt gaccagaaac tggtccttct gtttgtcacc gttgtcgaac
     2461 tgaaactccg cccatagcac atacgtccca ggcgaaagat ctttcggcaa gttaagatgc
     2521 accatcgcac gagcctgttg gcctgtttcg acagaaagcg tatcaccacc ttgcagaagc
     2581 acggatgccg gtcttatctc ctcttggatg agtacgggaa gatgcccctt gcctttgatt
     2641 ctcgcggctt ggtgatctgg cacggtaccg aattgccaga tgtatttcac ctctgccggc
     2701 tcgcgtgagt tatttatgac aatgagctgt ttttcgatga cctcgcccgg taggtaattg
     2761 tggtccttag cggtgaaagc agtgctaccg ccggcgatgt aggccaatag tggagcattg
     2821 ttgcggtaca tggcttgggc ggccaaggtg ggaacccaat cggatggctc ataggcgatg
     2881 tccattcgct cgtaccgtac ctcaaggtag tcggggctat aacctggtct ttgcaggttt
     2941 tcccagtcgg tggaaagttc ggtccgctgg ttccgcgcta accccggccg cattttccaa
     3001 tagatatgat actcccatgg agaaatggcg gaaacacccc aggtgcgaaa tgcccgccag
     3061 ttatccgtca aatactgcgc gaaaatagga tatcgctcgt cgaagtcact ggatcccaac
     3121 tggtgcggat agtcccagcg ataccaaaga cgacctgagc gaaactgcgc ggcttcccac
     3181 ttgaggttcc gtctctcctg gtcactgatg ttgtaagctc ggtcgccgag gaattgcgcg
     3241 ttccactccg ccaggcagaa gtcccatggc acaactgcgc tgccaaactc ccgctgtccc
     3301 ttgtaccaac cccgatacat cgcccaatcc caggtg
//
LOCUS       DAOSUT010000273         3319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11746_length_3319_cov_8.460172, whole genome shotgun sequence.
ACCESSION   DAOSUT010000273 DAOSUT010000000
VERSION     DAOSUT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_3319_cov_8.460172"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1582
                     /locus_tag="PLD05_15485"
     CDS             275..1582
                     /locus_tag="PLD05_15485"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOP78887.1"
                     /translation="MRNSCYWLGVRFLVALFTWVCLRSIVVPAEEQPLIAKQACCVLE
                     LPEKSLRLPVRAALPAGCSVREGRHCTVVSQAPDLKVSAEVLGGPDANGELRAPGYLV
                     LRSTSAEKVSQDKVQPISLQIHVNEGSGKSTDTFRWDETSEVTLTLRDDHGNPVLAYN
                     FGPITNPSVPANDPRRTRACYIHPVWGLKGEVLTDDFPRDHYHHHGIFWTWPHVVIRD
                     QEYSLWEDKGALRQRFRKWLCREAGEHAAVLGVENGWMIDEECLLAERVWITVYKADS
                     LGRMIDIDLFLLPHVPVTLWGAEGKSYGGLTMRFRPPSQKDAMITVPAGLTGTDLYET
                     RLPWADFTSLFGDDGASLSGGTIMVAPDHPDYPPTWLTRHYGPLCIGWPGIQSRTLEP
                     GSCTRLSYRIWIHSDLRTAAEIHKIYEMFCAAAKLVCQKSDSQ"
     gene            complement(1697..2314)
                     /locus_tag="PLD05_15490"
     CDS             complement(1697..2314)
                     /locus_tag="PLD05_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOP78888.1"
                     /translation="MATILEPFELEALDSLPFDSAAYITPSESTIASLVRRAKDGDNE
                     AWEQLIQRYQKLVYDTVFAKLRSHSDAEEVCQEVFLQALRKLFQLEVPEAFGSWLKRI
                     AERLAINRLSRGRSEYALTDTQMERQRDLRAEPVREVMRREAQQRVLCRLNDLRSMDR
                     EALVAFYLEGKSLAEISHELAVPVGTVKRRLHVARKRLAKLLEQE"
     gene            complement(3025..>3319)
                     /locus_tag="PLD05_15495"
     CDS             complement(3025..>3319)
                     /locus_tag="PLD05_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78889.1"
                     /translation="NSEGREIDFGGVITAQGLRVTREDRGIRVMALPFEDRATTAKII
                     WKKLPWRLPPPQRIEYRDLDGQVIKTEAVSGDVSPVSISIVPPAVDCRIVTEP"
BASE COUNT          743 a          938 c          897 g          741 t
ORIGIN      
        1 cagtcgcgat ggcaaaacaa ggcccgcaat cgcagccgtc gctccgcact tgagataatc
       61 ccggcgagac agactgacgt tggtttttcg catggatggt tctccttttc tgaacgggca
      121 ctttgtgggg aaggcatcac tatgacacgc cgtggcaaac aggaaggtaa cgttaccggg
      181 gctacgggtg ttccgttgca atcgcccgtg attataatca ccggttgttc gtggtgacaa
      241 gcgactgggt atcgaagctt ggatagggtt agatatgcga aacagttgtt attggttggg
      301 tgttcgattt ctcgtcgcgt tattcacttg ggtttgttta cgatccatcg tcgtgcctgc
      361 cgaagagcaa ccgctcattg ccaagcaagc ctgctgtgtg ctcgagcttc cagagaagtc
      421 cctacgcttg ccggttcgtg cggcactgcc tgcgggttgc tcagtccgcg agggcaggca
      481 ttgcactgtc gtctctcaag caccagacct gaaagtatcc gcggaggtcc tgggtggccc
      541 ggatgctaat ggggaactcc gtgcaccagg gtatctagtt ctcagaagta catccgccga
      601 gaaggtgtct caagacaagg tccagcccat ttctctgcaa attcacgtta atgaaggctc
      661 cggcaagtca actgacacat ttcgctggga cgagacctcg gaggtaacgc tcaccctccg
      721 tgacgatcat ggtaacccag tgctagcgta caactttggg cccatcacga atccttccgt
      781 cccggctaac gatccgcgtc ggacgcgggc atgctatatt catccggtgt ggggacttaa
      841 gggcgaagtc cttacagacg actttccacg tgatcattac catcatcatg gcattttctg
      901 gacctggccc cacgtggtga tccgcgatca ggagtatagt ctctgggaag acaaaggagc
      961 attgcgacaa cgatttcgca agtggctttg ccgcgaagcc ggtgagcatg ccgcggtgct
     1021 gggcgtggaa aatggctgga tgatcgacga agagtgtttg ctcgcggaac gggtgtggat
     1081 cacagtgtat aaagcggatt cgctggggag gatgatcgac atcgacctgt ttctcctccc
     1141 ccatgtcccg gtcacgcttt ggggtgccga gggcaagagc tacggcggtc tcacgatgcg
     1201 gtttcgaccg ccttcacaaa aagatgctat gattacagtc cccgcaggct taacgggcac
     1261 tgacctttat gaaacacgct tgccctgggc cgacttcaca agcctttttg gtgacgatgg
     1321 ggccagtcta agcgggggaa ccatcatggt ggcacccgac catcccgatt acccgcctac
     1381 ttggctcacc cgccattacg gcccgctttg catcggttgg ccggggattc agagtcgaac
     1441 actcgagccg ggaagctgca cgcggctgtc ttaccggatt tggatccatt ctgatcttcg
     1501 cacggcagcc gaaattcaca agatttacga gatgttctgt gccgccgcaa aacttgtatg
     1561 ccagaagtca gactcacagt gaggatgaca cagacccttt gacccgcaag gcgacaacgg
     1621 tatgcgtcgg cttgggtgat aaacccgctt ggcgccgtga gcgttctacg aacagaggcc
     1681 ggctgagtgt acaatatcac tcctgctcaa gcagtttggc cagccgtttt cgtgccacgt
     1741 gcagccgtcg tttgaccgta cccacgggaa ctgcaagttc gtgactgatt tccgcgagtg
     1801 atttaccctc aaggtagaag gcgacaaggg cctcgcggtc catcgatcgg aggtcattca
     1861 gacggcagag aacccgttgc tgagcctctc gtcgcatgac ttcccgaacg ggttcggccc
     1921 gaaggtctcg ttgcctctcc atttgggtgt ccgtcagtgc atattccgat cgaccgcggc
     1981 tgagacgatt aatagccaag cgctccgcga ttcgtttcag ccaactccca aaggcctccg
     2041 ggacttccag ttgaaacagt ttgcgaaggg cttgcaggaa gacctcctgg cacacctctt
     2101 cggcatcgga gtggctccgc agcttcgcaa agacggtgtc gtaaaccagc ttttgataac
     2161 gctggatcaa ctgttcccaa gcctcgttat caccatcctt cgcacgccga accaaggagg
     2221 cgatagtgct ttcggacggg gtaatatagg ctgcggagtc gaaaggtagg ctatcgagcg
     2281 cttcgagttc aaagggttcg agaattgtag ccatgttatc ctccggcaaa tgggtggatg
     2341 tgagccaccg ccctttgaga gccggccctg cgaaaacgcg aatcaacacc acatcgatcc
     2401 gttttcacag gaccgagggt gtcggttcgc atccaccgtt atctagacaa tgacagctaa
     2461 tcttgtggcg gacgcgacca acaccatcgg ctctcccgac gcgcaaagcg tcgttgataa
     2521 gccggtgcaa gagcccaggc cctatgcatg cgcacgcaac accccaactg ggtggttgca
     2581 tactgcaacg cctgggacag caaggtgacc gcgcaggaga tgtgtttaac gaccgtacga
     2641 acgacaacgc ccttgctcac gtgacgctta ccgaccaacg tgaccaacgt cttgacggcc
     2701 ttgatcgttc gcacgaacgt acgcataaca tcacctctgc gcgaaataga agacctcaaa
     2761 acccgtaaac gtcaagaaaa caccccaatc ggtgttaaac cttattctac ccaaactggc
     2821 aaaacccggc cagctttggc cataagttaa gtttaaaggg cataacctgc ctacaaacgt
     2881 tatcgttaat aggacgcaca cctggcatta tcggttcgca atttttcatg catgctccaa
     2941 tagtacacat tgtcctagcc aataaatcac cagatgtgca catatcgtgc gtccacgtgc
     3001 cagtacttgg ccatttcctc gtcgctatgg ctcggtcaca atccggcagt caactgcggg
     3061 cggaacaatg gaaatcgaca ccggtgagac atccccagag actgcctcgg tcttgataac
     3121 ttgcccgtca aggtcacggt attcgatccg ttgcggcggc ggcaatcgcc atggcaactt
     3181 tttccaaatg atcttggctg ttgtggcccg gtcttcaaaa ggcaaggcca tcacgcgaat
     3241 cccgcgatct tctcgagtca cgcgcagccc ctgggccgta attacgcccc cgaaatcgat
     3301 ctctcggcct tccgaattc
//
LOCUS       DAOSUT010000274         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_11941_length_3282_cov_8.956306, whole genome shotgun sequence.
ACCESSION   DAOSUT010000274 DAOSUT010000000
VERSION     DAOSUT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11941_length_3282_cov_8.956306"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3059)
                     /locus_tag="PLD05_15500"
     CDS             complement(<1..3059)
                     /locus_tag="PLD05_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOP78890.1"
                     /translation="MAGLEETIRDLCHQLDRAIRHREARRVEDYLATLDVSQLETDLL
                     LELIYTEFVARSETGESPQADEYYERFPYLKDDLQELFFVHNLVAASLHSLDSTKEEN
                     DAEGEPSDLVANSFDAPATPAEGQSGSSPFETTTGEGFAAEGTVSESGVRGTILGHYE
                     LLEEIGRGATAVVFKARDLRLKRYVALKVINQASWDRAARERVLAEAQAAAGLRHPHI
                     VQIYEVGESGDTCFVAMEYLEGGSLADRPDRVWSPREAARLVERIARAVQYAHEHHIL
                     HRDLKPANVLLDLDGTPKIVDFGLAKRLDEVSPNGSQEDLVGTPSYMAPEQAAGEVGA
                     VGPATDVYGLGAILYELLAGRPPFTGKNAIETLLQVRHQDPVPPSRLQPYIHRDLVTI
                     CMKCLEKAPERRYPSAAALADDLQRFLEGHVILARPAGPVERLAKWVRRHSTAVLMGV
                     MTVFAVLGVIFGLRWHFARLQKIEQDRQTLAQLAEKQAEELRSQEDLSRQRQYVEALA
                     RAQELLGDNTAQALAILEDPEKCPEDLRDFAWHLLHRLCHEGRTYWTLAVNQEKWVRR
                     LTLSPDGQYLFWGTWDGTILVWNTLDQRVSHSFTAHSDWISAFAMVPQNNWLVTASYD
                     HTIRIWQWQPSDEDGPAQLLREFRLEGNDVAWDLTVHPDGQVLLAATDRGIGVFPLSP
                     QVTVESLLAKVNAEVVTVMPPRNAPKQASAGEGDRFFFLPPLATSTEKKTLIVHGQHR
                     LGQAGMFRSVKFSPQGRVVACGAVNGRIVLFSWPQLEILLSLREFQDPVLSIDISRDG
                     NLIAAAAAGNVWLYNMASKQSRYLRDVHFHAIRTVQFSQDGRRLLTAAEDYTAKVWDV
                     KSLAQSHRLTAHIGYAVYGTFSPDGRGLYTASQGMLNLWDLEKHDVPLRLGTDNRTTR
                     AMAWSPDGKHLATVEGTLWRDSSLSLWDVPTRNRLISAKQNGLSDDWLFFTRDGKWLI
                     SVVRERMVEIRDAQTLSVRRVIATLPAPISAMTLSPDGQSLLAGDRRG"
BASE COUNT          720 a          973 c          913 g          676 t
ORIGIN      
        1 ccgcggcgat cgcccgccag caaggattgc ccatcggggc ttaaggtcat ggccgagata
       61 ggggccggaa gtgtggcaat cacccgtcga acactgagcg tttgggcgtc gcgaatttca
      121 accatgcgtt cgcgcaccac agaaatgagc cactttccgt cgcgggtaaa gaataaccaa
      181 tcatcggata aaccgttttg cttagcacta atcaaacgat tcctcgtggg aacatcccaa
      241 aggctgagcg atgaatctcg ccaaagcgtt ccttcgaccg tggcaagatg ttttccgtcg
      301 ggcgaccagg ccatggcccg cgttgtccgg ttgtctgtgc ccaagcgcaa aggaacatca
      361 tgcttttcca aatcccagag gttcaacatc ccttgagacg ccgtgtaaag gccgcgtcca
      421 tctgggctaa aggttccgta cacggcataa ccgatgtggg ccgttaaacg gtggctctgg
      481 gcaagactct tcacatccca cactttggcc gtgtaatcct cggccgcggt caacaagcga
      541 cgtccatcct gggagaactg aactgtacgg attgcgtgga aatggacatc tcgcaaatac
      601 cgcgactgtt tggaagccat gttgtacagc cacacgttgc ccgccgctgc tgccgcaatt
      661 aggttaccat ctcgcgaaat gtcgatgctc aagacaggat cctgaaactc cctgagggaa
      721 agcaggatct ccaattgtgg ccaggaaaat agaacgatcc ggccgttgac agctccacat
      781 gccacgaccc ttccctgggg tgagaactta acagaacgaa acatgccagc ctgtcccagg
      841 cggtgctggc cgtgaacaat gagcgtcttt ttttccgtgg aagtcgccag gggtgggagg
      901 aaaaagaacc ggtcaccctc ccctgcggat gcctgtttcg gcgcatttcg cgggggcatc
      961 accgtgacta cctcggcatt caccttcgcc aggagcgatt ccaccgtaac ctggggacta
     1021 agagggaaca cgccaatacc ccgatccgtc gctgccagaa ggacctggcc atcgggatgg
     1081 acggtcaaat cccacgccac atcgttgcct tccagccgga actccctgag tagttgtgcc
     1141 ggaccatcct catccgaggg ctgccattgc caaatccgga tggtgtgatc gtaggacgcc
     1201 gtgacaagcc agttgttctg gggaaccatg gcgaaagcgg aaatccagtc actatgcgcg
     1261 gtaaagctgt gactgacccg ctgatcaagc gtgttccaaa ccagaatcgt cccgtcccac
     1321 gtgccccaga acaggtactg gccgtctggt gataacgtga gacggcggac ccatttctct
     1381 tggttgaccg caagtgtcca gtaggtcctg ccttcatgac acagacgatg gaggaggtgc
     1441 caggcaaagt cgcggaggtc ttcgggacac ttttccgggt cttccagaat ggccagcgcc
     1501 tgggccgtat tgtcgcctaa caattcctgg gcacgtgcca aggcctcaac gtattgtcgc
     1561 tgacgactga ggtcttcctg cgagcggagt tcttccgcct gtttttcggc gagttgcgcc
     1621 agggtttgac gatcctgttc aatcttttgc agtcgtgcaa aatgccaccg caggccaaag
     1681 atcacaccca aaacggcaaa gaccgtcatc actcccatga gaacggctgt ggagtggcgt
     1741 cgaacccact ttgcgagccg ttccaccggt cctgcgggtc gcgccaaaat gacgtgccct
     1801 tcgaggaatc gctgcaaatc atccgccagg gccgcagccg aggggtaccg gcgctctggt
     1861 gccttttcca agcacttcat gcagatcgtc acgaggtcac gatgaatgta cggttgcagt
     1921 cgcgagggcg gaaccgggtc ctggtggcga acctgaagga gtgtctcgat ggcattcttg
     1981 ccggtaaagg gtggccgacc tgccagcagt tcgtacagga tcgcccccag gccataaaca
     2041 tcggtggcag gtccaacagc tcccacctcg cccgccgctt gctccggggc catgtagctg
     2101 ggtgtaccaa ccaaatcctc ctgcgaacca ttgggcgaga cctcgtccag ccttttcgcc
     2161 agtccaaaat ccacgatttt tggtgtgcca tcaaggtcca aaaggacatt ggcgggtttc
     2221 aggtctcgat gaaggatgtg gtgctcgtgg gcgtactgga cggcacgggc aattcgttcc
     2281 accaggcgtg ctgcctcgcg tggcgaccac acccgatccg gacgatcggc cagcgatccc
     2341 ccctccaaat actccatagc gacgaaacag gtatcgccgg attcaccgac ctcgtatatc
     2401 tgaacaatgt ggggatgtcg caaccctgcg gcggcctgcg cttcggcgag gacccgctct
     2461 cgcgctgccc gatcccaact cgcctgattg atgactttaa gcgcgacgta tcgcttgaga
     2521 cgtaagtcgc gagccttgaa gacgaccgca gtggcccctc gcccgatctc ttctagcagc
     2581 tcgtagtgcc caagaattgt gccgcgaaca cccgactcgg aaactgtccc ctcggctgcg
     2641 aaaccttcgc cggtggtagt ttcaaatggg gatgaacctg attggccttc ggcgggggtc
     2701 gctggagcgt cgaacgaatt ggcgactaaa tccgaaggct ctccctccgc atcattttct
     2761 tctttggttg aatcaagaga gtgaagtgac gccgccacca aattgtgaac gaaaaacaac
     2821 tcttgcaggt catctttcaa gtaaggaaag cgttcatagt actcatcggc ctgcggcgat
     2881 tctccggttt cgctacgtgc cacgaattct gtgtagataa gttccagcaa aagatcagtc
     2941 tcaagctggc tgacgtccag ggtggccaga tagtcctcga cccggcgggc ttcccgatga
     3001 cgaattgccc gatcaagttg gtggcacaaa tcgcgaatag tctcttccaa acccgccatg
     3061 attgcagcgc ggtcccctat tgaggatgtg aacggggctt gcccctcttc tatattgtgc
     3121 cgctggcagg ccgaagaacg caaatcctgt ggaatccaaa gcccgcagaa ccccggcaac
     3181 gcagaaatcg gcggacgcag gggaccgcgt ccctcccaag ggcaacagag gactcacgat
     3241 cgggcaactt gacaatgcgc tcgtcctcgc tcacattttt gg
//
LOCUS       DAOSUT010000275         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_13579_length_2951_cov_8.342541, whole genome shotgun sequence.
ACCESSION   DAOSUT010000275 DAOSUT010000000
VERSION     DAOSUT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_2951_cov_8.342541"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>2951)
                     /locus_tag="PLD05_15505"
     CDS             complement(160..>2951)
                     /locus_tag="PLD05_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOP78891.1"
                     /translation="SVYLFRMVARQIGGGSGCVITGPATVNHDYRLTEAWREYSHIFR
                     MPDEPISKSLFRLGQWEMSGRVQFDKVELLPVLPIHARNNECVLGQGESIRDGIYSFE
                     GTFDGIGSNDHRVLHRATARFNTNRWVFSEDEEVVYKFQLPETKFLSGQLSFAVNYAT
                     GGTMLAEVSPDGTSWTEAARQESVGVRQAELPASLFPTPAIFVRLRHVGTAASMQVDR
                     IEFRGKIDRHLSDFQGITTYGVWRPLADSISHPAIDLHITGASGSMSRSLAVHPRDVP
                     EPKLDASVIDSEKKTHPIAVVPATSSAQNSGGWTITLPQLGPGQHLCRINLSSQGQTL
                     GVLEIPWEVNQYYRADYGELLSSQPDAVIWWCEADWKIPRDRPPPSGFPSPSSMVRLT
                     SARNDYESVQVVISPQADGRLKSVEVGLLRHPSGATIAGEDIKVSYVYYHYVHTPTDK
                     EGVRDWWPDALPPCDSPLSLVKGQNQPLWITVHVTEDKPAGDYTGQMTLTTDTSTTSI
                     PISLHVWNFALPKRNHLETAFGFDHSLAFRYHNVKTEEDQRRLLDLYFEIFSEHRISP
                     YNPTPLDPFVVRFDPQASPPSAKIDFSRFDRAMEKAIEVFHFTNFQLPIRGMGGGTFH
                     SRYEPEIAGYGESTPEYQVMFASQVQQIEAHLREKGWLNMAYVYWFDEPDPKDYEFVR
                     KGMDRLKKYAPQLRRLLTEEPVEPLYGAVDIWCPVTPNYNHEIAESRRKLGERFWWYV
                     CTGPKAPYCTLFIDHPATELRVWLWQTWQRGIDGILVWSTNYWTSSAAFPDGFQDPYE
                     DPMGYVSGYSTPKGVRRHWGNGDGRFIYPPLSASVPGKTSEPVIAKPVSSIRWEMLRE
                     GIEDYEMLWLLRGLLRKKGDRLPAERRKELEDLLAVPPTITTNMTTFTKSPKPIYEHR
                     SKVAEAIEKLSAAEP"
BASE COUNT          667 a          797 c          822 g          665 t
ORIGIN      
        1 ccggcgaccg cgacgaccag ttcctcttgg gttgcgattt tatcgtgacg ttctagccag
       61 aagttagtcg ttcgtatcaa taaacgaata cggcgctgga gtttcacaac ttcagcgccg
      121 tattcctttt tggccctctg ggctccaaat gctttgacct catggctctg cggccgacaa
      181 tttctcgatc gcttcggcca ccttacttcg atgctcataa atgggtttgg gcgacttagt
      241 gaacgtcgtc atgttagtag tgatggtggg cgggacggca agcagatctt ccagctcttt
      301 acgccgttcc gcaggaaggc gatcgccttt cttccgcagt aaaccgcgga gcaaccaaag
      361 catctcataa tcctcaatcc cctcacgaag catctcccag cggatgctgg acaccggctt
      421 ggcgattacc ggttcggatg tcttgccggg cacagaggca ctcaaaggcg gatagatgaa
      481 ccgaccatct ccatttcccc aatgacgtct gacccctttc ggcgtggagt atccggagac
      541 gtaacccatg gggtcctcgt aggggtcctg aaaaccgtcg ggaaatgcgg cactggatgt
      601 ccaataattg gttgaccata ccagaatgcc atcgatcccg cgctgccatg tttgccacag
      661 ccacacccgc agttcggtgg ccggatgatc gataaaaagc gtacaataag gggccttggg
      721 acccgtgcac acgtaccacc agaaacgttc gcccagtttc cggcgtgatt cagcgatctc
      781 gtgattgtaa ttgggcgtga ccgggcacca aatatcgacg gcgccataaa gcggctcgac
      841 gggttcctct gtaagcaatc gtcgcagttg cggcgcgtat tttttaagac ggtccatgcc
      901 cttccgtaca aactcatagt ccttgggatc aggttcgtcg aaccagtaca cgtaagccat
      961 gttgagccac cctttctcac gcaagtgggc ctcaatctgt tgcacttggc tggcaaacat
     1021 cacctggtac tcaggagtgg actcaccgta cccggcaatc tctggttcgt agcgactgtg
     1081 gaacgttccg ccacccattc cccgaatcgg caattgaaaa ttggtgaagt gaaagacctc
     1141 gatggccttt tccatagcgc gatcgaatcg ggaaaaatcg atcttcgcag acggcggact
     1201 ggcttgggga tcgaagcgga ccacaaacgg gtccaaaggt gttgggttgt acgggctgat
     1261 acggtgttcg gagaatattt cgaaatacaa atcgagcaat cgcctctgat cttcttctgt
     1321 tttgacgttg tggtaacgaa aggccagcga atggtcgaat ccaaacgctg tttcgaggtg
     1381 gtttcgcttc ggcagcgcaa aattccagac gtgaagcgat attggaattg aggtcgtcga
     1441 agtgtcggtc gtcagggtca tctgacctgt gtagtccccc gccggcttat cctcggtcac
     1501 atgaacggta atccacagtg gttgattctg acccttgacc agagaaagcg gggagtcaca
     1561 gggaggtaag gcgtcgggcc accagtcgcg aacaccttcc ttgtcagtcg gcgtgtgaac
     1621 gtagtggtaa tacacgtaag agaccttgat atcctcaccg gcaattgtgg cgccagacgg
     1681 atgcctcaag agcccaactt ccacagattt cagtcgtccg tcagcttgag gcgaaatcac
     1741 cacctggacg gattcataat cgtttcgagc cgaggtaagg cgaaccatcg aactcgggct
     1801 cgggaaacca cttggcggcg gccgatcgcg gggaatcttc cagtcggctt cacaccacca
     1861 aattaccgcg tcgggttggc tgctgagtag ttccccgtag tcggcgcggt agtactgatt
     1921 gacttcccat ggaatctcga ggacaccaag tgtttggccc tggctcgaaa gattgatacg
     1981 gcaaagatgt tggcccggac caagttgcgg caatgtaatc gtccagccgc ctgagttctg
     2041 ggccgaagat gttgccggga cgacagcgat aggatgcgtc ttcttctcag aatcgatgac
     2101 tgaggcatcc aacttgggct cgggcacatc tcgtgggtgc acagcaagag accgggacat
     2161 cgagccgctc gcaccggtaa tatggagatc aatggccgga tgagaaatgc tgtcggcgag
     2221 gggacgccaa accccgtagg tggtgatgcc ctggaagtcg gagaggtgac ggtcaatctt
     2281 accgcggaac tcgatacgat cgacctgcat cgaggcagct gttccgacgt ggcgaagcct
     2341 gacaaagatg gccggtgtcg gaaagagaga tgcgggaagt tctgcctgtc ggacgccgac
     2401 actttcttgt cgtgcggctt ccgtccatga tgtcccgtcg gggctgacct cggccaacat
     2461 ggtgccaccc gtcgcgtaat ttacagcgaa actgagctgg cccgacagga actttgtttc
     2521 tggtagctgg aatttgtaaa ccacttcctc atcttccgaa aagacccagc gatttgtgtt
     2581 gaacctggcc gtggcccgat gaaggacccg atggtcgttg ctgccaatac catcaaatgt
     2641 tccttcaaac gagtagattc cgtcgcgaat gctttctccc tggcctaaga cacattcgtt
     2701 attgcgggca tgaataggca gaacgggcag aagttcgact ttgtcaaact gaacacgtcc
     2761 tgacatctcc cactgaccga gcctgaaaag actctttgaa attggttcgt ccggcatgcg
     2821 gaaaatatgg ctgtactcgc gccaggcctc ggtcaaccgg tagtcatggt taaccgtagc
     2881 ggggcctgtt atcacgcagc cggacccacc accgatttgg cgggcgacca ttcgaaaaag
     2941 atagaccgag c
//
LOCUS       DAOSUT010000276         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_13583_length_2950_cov_10.155786, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000276 DAOSUT010000000
VERSION     DAOSUT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13583_length_2950_cov_10.155786"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLD05_15510"
     CDS             <1..501
                     /locus_tag="PLD05_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOP78892.1"
                     /translation="VVREVVTREVRIPPGVDQNTRLRLQGEGELSLDGGPRGDCYCLI
                     RIKEHPLFKRDGANLVCRIPISYAQAALGATIEVPTLDGPETLTIPPGTQSEQVFVLP
                     GKGMPTRSRHRGNLLVQVYIEVPKKLTPKHERLLRELAELENNEVLPERKSFFAKLKE
                     YFAGGS"
     gene            597..1367
                     /gene="grpE"
                     /locus_tag="PLD05_15515"
     CDS             597..1367
                     /gene="grpE"
                     /locus_tag="PLD05_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOP78893.1"
                     /translation="MSKKQTNNHSNNPEHVKEEHLQNATPDRPESDARNSASVQEERD
                     TNEVPTAEAEVMSDEQIQQPTPEQNAGTISAEDYEKEIAALKQEAEEFRNRAMRWQAE
                     LENYQKRIARQLAEERRYAAIDLIRDLLPVYDNLQRAAQSAEQSHDVAQILQGLRMIL
                     KQWEDVLARHHCQKIDALQKPFDPNVHHAISRQATNDVPPNTVICVAQDGFILHDRVV
                     RPSQVVVSMALPTAGSEGSSEAVPSSPGDESPGDHPPS"
     gene            1452..1736
                     /locus_tag="PLD05_15520"
     CDS             1452..1736
                     /locus_tag="PLD05_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOP78894.1"
                     /translation="MPTYEYVCDACEHRFEEFQSITADPLTKCPACGKKKLRRLIGTG
                     GAILFKGSGFYCTDYRSESYRKAAARDNGNGSSSSKSSETSSSTKSNGKD"
     gene            1931..2947
                     /locus_tag="PLD05_15525"
     CDS             1931..2947
                     /locus_tag="PLD05_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78895.1"
                     /translation="MAVPQDRRQFLQQAGLIAAAGAAVSLEEKILLAGVQQEKTGEAE
                     QSASNLAAEMPHGKVRNISISRLVIGGNLIGGFAHSRDLVYVSRLLKAYNTEEKVFET
                     LSLAERHGINTIQCNPACLPLVEKYNKERGGKLQAIVCITPDADKDTVRREIDEVLSH
                     GATMLYTHGGRSEPPVMANRLDVLANTIECIKAAGVPAGIGSHSLTVTIECEKNGIGA
                     EFYVKTLHSDQYWSATPKELREDFCWQKPRRDEPFAFHDNMWCLNPEETIEFMKSVSK
                     PWFAFKILAAGAIHPRIGFPYALRNGADFLIVGMFDFQIAEDTAVFLESFKKLGRRER
                     PWCA"
BASE COUNT          746 a          750 c          845 g          609 t
ORIGIN      
        1 gtggtccggg aggttgtcac ccgggaggtc cgtatcccac cgggcgtgga tcaaaatacc
       61 cgtttgcggt tgcaaggtga gggggaattg agcctggatg gtggaccccg cggtgattgt
      121 tattgcttaa tccgcattaa ggagcatccg ctatttaagc gagatggtgc caacttggtc
      181 tgccggattc ccatcagtta tgcccaggcg gcgctggggg ccacgattga ggtaccgacg
      241 ctcgatgggc cagaaacgtt gacgatcccc ccagggacgc aaagcgagca agttttcgtg
      301 ctacccggca aagggatgcc gacgcgatca cgccatcgcg gtaacctgct tgtgcaggtt
      361 tatatagagg tgcctaagaa actcactccc aagcacgaac ggttgctacg tgaattggcc
      421 gagttagaaa acaacgaggt gttgccggaa agaaaaagct tctttgcaaa gcttaaagag
      481 tactttgccg gtgggtcgtg aagactgaaa agttaaaagc tccttccctc agtcaaagtt
      541 ggggcggata cccttgggtc gaaaactcaa cctaggtgac tggaaaggag ccgaccatga
      601 gcaagaagca aacaaacaat cattcgaata acccagagca tgtcaaagaa gaacatcttc
      661 aaaacgcgac tcctgatcgg cctgaatcgg atgcaaggaa ttcggcatcc gttcaggaag
      721 aaagagacac aaacgaagtc ccaacagcag aggcggaagt aatgtcagac gaacagattc
      781 aacaaccaac tccggaacag aatgccggga cgatctcagc agaggattac gagaaggaga
      841 ttgcggccct gaaacaagag gccgaggagt tccgcaatcg ggccatgcga tggcaggctg
      901 agctggagaa ctatcaaaag cggatcgccc ggcagttggc cgaagaaagg cgatatgcgg
      961 ccatcgattt gattcgtgac ctcttgcctg tctatgacaa tttgcaacga gcggcgcagt
     1021 cggctgagca aagccatgat gtggcgcaaa tcctgcaagg attgcggatg atcctgaaac
     1081 agtgggagga cgtcctggcg cggcatcatt gtcaaaaaat tgatgccctg cagaaaccat
     1141 ttgatcctaa cgttcaccat gctatctctc gtcaagcgac gaacgacgtc ccgcccaata
     1201 ccgtcatctg tgttgcccag gatggattta tccttcacga tcgggtcgtt cgcccaagtc
     1261 aagtcgttgt ttccatggct ctgcccacgg cgggcagcga aggaagcagt gaggctgtgc
     1321 cgtctagtcc gggcgacgaa tctcccggtg atcatccgcc aagctaaggc ttcaaacttt
     1381 ggcgccaact gggcatgttg tgaaattctg accacggata ctcactcatt ttttagaata
     1441 aggaggtctt tatgccgaca tatgaatacg tttgtgacgc gtgcgaacat cgttttgaag
     1501 agtttcaatc gattacggca gatcctctca ccaagtgtcc cgcttgcgga aaaaagaagc
     1561 ttcgccgcct gatcggcacc ggtggggcca ttcttttcaa aggttccggt ttctattgca
     1621 ccgattatcg gagcgagtcg taccgcaagg cagcggccag ggataacggc aacggctcaa
     1681 gttccagcaa atcttcggag acgtcttcca gcaccaagag caacggaaag gattgacgcc
     1741 acgagtcccc gcattcgcga tgataaaaac tgagtagggc tctgcgcgct ttttgccgcc
     1801 aagggtgtga cagggggcac gccgtttggt aaaatcacgc tagcacggcc aatgagagag
     1861 tcgcttggcc gatggtgccg gtgttgaaag atgtatgtcc attaagtcgc ggatgcgggg
     1921 aggaccgaaa atggctgtcc cacaagatcg gcggcagttc ctgcaacagg cgggtcttat
     1981 cgcggcggcc ggtgcggccg tcagtctaga ggaaaaaata cttctggcag gggtccagca
     2041 agagaagacc ggggaagcgg agcagtccgc ctcaaacctg gcggcggaga tgcctcacgg
     2101 caaggtccgc aatatttcga ttagccgtct cgttatcggc ggaaacctga tcggcgggtt
     2161 cgctcacagc cgggatttgg tgtatgtatc ccggctcctc aaagcatata acacagaaga
     2221 aaaggttttt gagacactat cactggcaga gaggcacggg atcaacacga ttcagtgcaa
     2281 tccggcgtgt ctgccgctgg tggaaaaata caacaaggaa cgcggcggta aactccaggc
     2341 cattgtttgt atcacccccg atgcggacaa ggacaccgtg cgtcgggaaa ttgatgaggt
     2401 cctcagtcac ggggcaacga tgctctatac gcatggcgga cggagcgaac ctcccgtcat
     2461 ggccaatcgg ttggatgtcc tcgccaatac gatcgagtgc atcaaggcgg cgggtgtgcc
     2521 ggcaggtatc ggcagccatt ccctgaccgt taccatcgaa tgcgaaaaaa atggcatcgg
     2581 tgctgagttc tacgttaaaa cgctccacag cgaccaatac tggtcggcca cgccaaaaga
     2641 acttcgcgaa gacttttgct ggcaaaaacc aaggcgagat gaaccgttcg ccttccatga
     2701 taacatgtgg tgtctgaatc ccgaggaaac aatcgagttc atgaagtccg tttctaaacc
     2761 gtggtttgct ttcaagattc tggcagccgg tgcgatccac ccccgaatcg gcttcccgta
     2821 cgccctacgt aatggggccg attttctgat cgtggggatg ttcgattttc agatcgccga
     2881 ggataccgcc gtctttttgg aatcgttcaa gaaactgggc cgacgcgaac gaccgtggtg
     2941 cgcatgacgc
//
LOCUS       DAOSUT010000277         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_13965_length_2886_cov_9.647474, whole genome shotgun sequence.
ACCESSION   DAOSUT010000277 DAOSUT010000000
VERSION     DAOSUT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13965_length_2886_cov_9.647474"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PLD05_15530"
     CDS             complement(<1..1042)
                     /locus_tag="PLD05_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOP78896.1"
                     /translation="MRIDNRRLFCLAGAICSHLLMLAMVFAAWGVWTTVAYGAEDWPQ
                     FHGPNRDNICTETDLLESWPEGGPKLLWQLEGLGRGYSTVSIAGGKLFTMGDRRTEDG
                     EAQFVLAFDLAIRKELWATRIGLPHRDGGPRCTPTVDGPRVYAIGTDGDLVCLETATG
                     KVIWKKNFAQDFGGRMMSGWRYSESPLVDGEKLVCTPGGKNALIVALNKETGDVIWRC
                     DPGNLGPQGQDGAGYASLIPAVICGVRQYVTIVGRGAVSVDAETGRLLWNYNRIANGV
                     ANIPNPVIRNDYVFVTTAYRTGCALLHILRDGEKWTAEEVYFYDANHFANHHGGVVLV
                     GDYLYGGDGQNRG"
     gene            complement(1757..2605)
                     /locus_tag="PLD05_15535"
     CDS             complement(1757..2605)
                     /locus_tag="PLD05_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOP78897.1"
                     /translation="MKKTKRRNPEENGVNSPLEAYLREINETPLLTPEEEMDLAIAIG
                     QGDPVARDRMVRANLRLVVNIARNYVGKGLSLQDLIEEGNLGLLRAVEGYDPAVGTRF
                     STYASYWIKQSIKRALINCGKTVRIPAYMVEILSKWRRASTRLAEELGRTPTPEEVAR
                     VLGIPRKKLSIIKKAIRIYNLAPQTDQAENGWSLGEMLEDERFQSPDEQLVTHDSLQH
                     IREKLEKMDPREAAVLRMRFGLDDRQPRTLKEIGNILGLTRERVRQIEAEALSKLAQA
                     LNAGEP"
BASE COUNT          647 a          897 c          725 g          617 t
ORIGIN      
        1 caccacgatt ctggccatct ccaccataga gataatcacc cacaagcacg acgccgccgt
       61 gatggttcgc gaagtgattg gcgtcgtaga aatagacctc ctccgccgtc catttttcgc
      121 catctcgcaa aatgtgaaga agggcacacc ccgtgcggta agccgttgtc acaaagacgt
      181 aatcattccg aatgaccgga ttagggatgt tggccacacc attggcaatt cgattgtaat
      241 tccacaacag tcgcccggtc tccgcatcca cactaacggc cccccgccca acgatcgtca
      301 cgtactgccg aaccccacaa atgaccgccg gaataagaga tgcataaccg gcgccatcct
      361 gcccctgagg tcccaaattg cccggatcac atcgccaaat gacatctccc gtctccttat
      421 tgagggcaac gatcaaggca ttctttcccc ctggcgtaca gaccaatttt tcgccatcca
      481 ctaggggcga ttcactgtaa cgccaaccgg acatcatcct cccgccaaag tcttgggcaa
      541 agttcttttt ccaaatgact ttgccggtcg ccgtttcgag acaaaccagg tcaccgtccg
      601 tgccaatggc gtacacccgc ggaccatcga cggtcggcgt gcaccgagga ccaccgtccc
      661 gatgcggcag gccaatgcgc gtggcccaaa gctcttttcg tatcgccagg tcgaacgcca
      721 ggacaaactg ggcctcgcca tcctcagtcc tccgatcccc catggtgaag agctttccgc
      781 ccgcaatgga caccgtcgaa tagccacgac caaggccttc cagttgccaa agtaacttcg
      841 gcccaccttc cggccaagac tccagtaggt cggtctcggt gcagatatta tcgcgattgg
      901 gaccgtggaa ctgcggccag tcttccgccc cataggcaac cgtggtccaa acaccccacg
      961 cggcaaagac catggccaac atgaggagat gcgagcaaat cgctcccgcc aggcaaaaca
     1021 accgtcggtt gtcgattctc atgacagact ccatcgattc gaggaaccgt taggaaagga
     1081 caatcaaaat tccgcccctg tggaaaagta aagtataagc aacaggggag ccgtttgcca
     1141 ttcgtgctcg attacgatct tgtcacgctg ccggctgtgg ggccatgggt ttgtcactcg
     1201 cgaaaaatcg cagcccccga ctagactcgc tgcacatgcg tcgttcggac gagtgacttt
     1261 cggtatgcag accaagatga gcgtcaatca ttgccccgag acgctagtgt aagctggcga
     1321 tggttcataa aggaccacgc gtgttccatt tccactaggc gcgacccaca ggctgcctct
     1381 agagtttgcc tctacagttt cgtcccctca tgaccgcgcg tgaccaccag ggtaaatgct
     1441 ggtccaagat caaacttgga gaggcgcaca tcgtggtggc caagttgttt cgagccgccg
     1501 gaccaaacca gtggcacaca agtcgtatta cctggcccgt gctcaaattg tggacaatta
     1561 cgccttcgtt gcatttcccc tggttaaagc ctatcccaaa attccctctc cctctgggag
     1621 agggttaggg tgagggtaag ccatcgtcgg ggtcgatcga gaaaaccagc ggcgattcga
     1681 gccaccctca cccccggccc ctctcccgcc gaacgggcga ggggaggttg cgggacaggc
     1741 tcttacgcaa caggtgttaa ggctcccctg cattcagagc ctgagccaac ttgctcaggg
     1801 cctccgcctc aatctggcgg acccgttccc tcgtcaatcc caaaatgttg ccaatctcct
     1861 taagcgtcct cggctgacga tcatccaacc caaagcgcat ccgtaagacg gccgcctccc
     1921 gcgggtccat cttttccagc ttctcccgaa tgtgttggag gctatcgtgc gtcacaagct
     1981 gctcgtccgg tgactgaaaa cgctcatcct ccagcatctc gcccaaagac caaccgtttt
     2041 ccgcctggtc ggtttgcggc gcaagattgt agatacggat cgccttcttg atgatggaaa
     2101 gctttttgcg gggaatgcct aagacgcggg caacttcctc cggagtcggc gtgcgaccaa
     2161 gttcctctgc cagacgcgta ctagcgcgtc gccacttgga aagaatctcc accatgtacg
     2221 ccgggatgcg gaccgtcttg ccacagttga tcagggcccg cttgatcgac tgcttgatcc
     2281 agtagcttgc gtaggtggag aaacgtgtcc ccacggcggg gtcgtaaccc tcaacggccc
     2341 gcagtaaccc caggttacct tcttcaatca ggtcctgaag gctgaggccc ttgcctacat
     2401 aattccgcgc aatattcact actagacgta aattcgcccg aaccatgcga tcccgagcaa
     2461 cggggtcgcc ttgcccgatg gcaatggcga gatccatctc ctcttctggt gtgagaaggg
     2521 gtgtctcatt gatctcccgt aggtatgcct caagtggaga gttcaccccg ttctcctcag
     2581 ggtttcgtcg ttttgtcttc ttcatagctg ccgacacatt ttgtgcaatc gcgacaacag
     2641 actttctgac ccaatcaagc tatcggtgaa ggcaacaagc atcctgcaat gttagagaaa
     2701 agcaacaagc tgcacggact gagactgtta tcgcaatttt gtcaacgacg catctcgcac
     2761 cagtcgtgca gaatgaatcg acgaggccgc tgggctcgga gggaatccgc gctctctgac
     2821 cgaaccacac gtgatgagtc cccatcgtgc ggtgttggtc cttttgaacc gattatgcgt
     2881 ttccta
//
LOCUS       DAOSUT010000278         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_14462_length_2808_cov_9.507446, whole genome shotgun sequence.
ACCESSION   DAOSUT010000278 DAOSUT010000000
VERSION     DAOSUT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14462_length_2808_cov_9.507446"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLD05_15540"
     CDS             complement(<1..845)
                     /locus_tag="PLD05_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78898.1"
                     /translation="MPVKNLLLSALIYVLTWHLEGPPADSSEPIALGDRLELFVDDLL
                     IEQSTGITWKVHRPVPHEVVLVADQPWEGNTSAYFAVFQDGTRFRMYYRGSHYDTEQK
                     RSAHPEVTCYAESRDGIHWEKPRLGLFEFAGSSENNIVWMGPGSHNFTPFKDMNPACP
                     PEARYKALAGIKEYGGGLLALQSPDGIHWACVQESPVITEGAFDSQNLAFWDGHAQCY
                     RAYWRIFKNGVRDIRTATSKDFLHWEPWRDLNYGDAPREHLYTNAILPYDRAPHILLG
                     FPTRFL"
     gene            1049..>2808
                     /locus_tag="PLD05_15545"
     CDS             1049..>2808
                     /locus_tag="PLD05_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78899.1"
                     /translation="MNLCIYKPMSCVLVVFFLSSAGIFQTSSFGESAESTGNWYERSF
                     FLLHLDHHTTAQMAVGREADPEVTERLLAMVRPDVIQIHAKGNPGWTTYPSAVGFTPP
                     LLERDVMKVWTDIAKKDGYVFSAYFNIGRDREIMRRHPEWNRVRADGTPWDNMLCYHS
                     GVAESYLWPMLDEIMDRYHPAGFWLDGSCFTVRNCYCAKCRERFQQEFGLSPPTNPQA
                     PGWKEFKEMHRQIYREFCARTAARIKKRDPTCLVAFNWAYSLLMPEEPPPGIDYFTGD
                     HGCQVDELAADAIWYDSQGRPFDLMTTVWYADSQGQHVKPAKQIQQEMAVIIAHGGRY
                     FAWDNPTPESGLRPERYRMMADVVVPFLRSRQAWCLGTTALPDVALFHGAAAHYFLNE
                     VTPVSFPRNNPPLLTACEALRRLHLTPEMISDRRLENGDIRAKLLIFEDTPLLTDENR
                     QGLRRYLENGGRVLLTGRAVSQSKLWDSGTLSAIEHRNIGRGEVYFLPSVLFAGEEGS
                     TSQSSVRILDRMLPSKERRLVTDAPSTVEVTLRRRGNEMIVHFVNIAAGKRARDPATN
                     PFVALRVEELPPAPACRVSVA"
BASE COUNT          608 a          741 c          821 g          638 t
ORIGIN      
        1 agaaaccgcg tgggaaaccc caataggata tgcggggcgc ggtcgtaagg gaggatcgca
       61 ttggtgtaga gatgctccct gggggcgtcg ccataattga ggtcccgcca gggttcccag
      121 tgcaagaagt ccttggaagt tgccgtgcgg atgtcgcgga caccattctt gaagatccgc
      181 caataagccc ggtaacattg ggcgtggcca tcccaaaatg cgaggttctg tgaatcgaac
      241 gcgccctccg tgatgaccgg ggactcttga acgcaggccc aatgaattcc atcaggcgat
      301 tgcaaggcca gcagtccccc accatattct ttgatgcctg cgagagcctt gtaccgtgcc
      361 tcgggcggac aggcggggtt catgtcctta aacggcgtga aattatggct gccgggcccc
      421 atccaaacga tgttgttttc gctcgagccg gcaaactcaa agaggcccaa acgtggtttt
      481 tcccagtgaa taccgtctcg gctctcggca taacaggtga cctcaggatg tgccgaacgc
      541 ttttgctccg tatcgtaatg cgagccgcga tagtacatcc gaaagcgagt accgtcctga
      601 aaaacagcga agtacgcgga agtgttcccc tcccatggtt gatcggcaac tagaacgact
      661 tcatgaggaa cgggacgatg cactttccat gtgatgcccg tgctttgctc gatcagcagg
      721 tcatccacga acagttccaa acgatctccc aaggcaattg gctcagatga gtccgcggga
      781 ggcccctcaa gatgccaggt gaggacatag atgagagccg ataacaaaag gttcttgacg
      841 ggcatcggca gttctccttt tgcgacgacg cgaggtcgtg tcacccttgt tactcaggag
      901 ggctggtctt acttgggttg accgatgcga ttctaagtcc tatcgttgcc tcacgctagt
      961 tgccccaagg ggtgcattgg gatatgctgc atctcgaaca tgtcttaggt gagagcgttc
     1021 cgtggacggc acagttaagg agtgctgcat gaatctgtgc atttacaagc ctatgtcttg
     1081 cgtgttggtt gtgtttttcc ttagttcagc cggtattttc caaacgagtt ccttcgggga
     1141 gagcgcagaa agcacaggaa attggtatga aaggagtttc ttccttctgc acctcgacca
     1201 tcacacaacg gcccaaatgg cggtgggacg tgaggcggat cccgaggtca ccgagagact
     1261 gctggccatg gttcggcccg acgttatcca aatccacgcc aaaggtaacc ccggctggac
     1321 gacatacccc agtgccgtgg gcttcacgcc gcctttgcta gaacgagacg tcatgaaggt
     1381 atggacagac atcgcgaaaa aggatggata tgtattttcg gcatacttca atatcggacg
     1441 ggaccgggaa atcatgcggc gtcaccctga atggaatcgc gtccgcgccg acggtacccc
     1501 ctgggacaat atgttgtgct atcactcggg cgtggccgaa tcttatctct ggcccatgct
     1561 tgatgaaatc atggaccgct accatccggc gggtttctgg cttgatgggt cgtgctttac
     1621 ggtccgaaat tgttattgtg cgaaatgccg cgagcgattt cagcaagagt ttggactatc
     1681 gccgccgacg aacccgcaag ccccgggttg gaaagagttt aaggagatgc accgacaaat
     1741 ctatcgtgag ttctgcgcgc ggaccgcggc tcgcatcaag aagcgggacc caacgtgttt
     1801 ggtggctttc aattgggctt attccctgct tatgccggaa gaacccccgc cgggtattga
     1861 ctatttcaca ggcgatcatg gttgccaggt agatgaattg gcggcagatg cgatttggta
     1921 tgattcgcag ggacgaccct tcgatcttat gacaaccgtt tggtatgcag attcccaggg
     1981 acagcatgtc aagccagcga aacagatcca gcaggaaatg gccgtgatca ttgctcatgg
     2041 tggccgatat ttcgcttggg acaaccctac acctgagtcg gggctgaggc ctgaacgcta
     2101 ccgaatgatg gctgacgtgg tggtcccgtt tcttcggtct cgtcaggcgt ggtgtctcgg
     2161 gacaactgct ttgccggacg tggcgttgtt tcacggggcc gcggcgcact acttcttgaa
     2221 cgaggtgact cccgtgagct ttccgcgaaa caatccgccc cttctgacgg cctgcgaggc
     2281 tctccgccgt ttgcacttaa ccccagaaat gatttccgat cggcgtttgg aaaacggcga
     2341 catacgcgcc aagttgctga tctttgaaga tacaccttta ctgacggacg agaatcgtca
     2401 gggcttacga cgatacctgg aaaacggtgg gcgggtgctg ctcacggggc gagcagtctc
     2461 gcaatcgaaa ttatgggaca gtggaacgtt atcggcgata gaacaccgca atatcggccg
     2521 cggtgaggtg tattttctcc cgagtgtcct ttttgcggga gaagagggct ccacaagtca
     2581 gtcgtctgtc cgaattctcg accggatgtt accttccaag gaacgccggt tagtgaccga
     2641 tgccccaagc acggtggagg tcacccttcg ccggcgggga aacgaaatga ttgtccattt
     2701 tgtgaacatc gccgccggga agcgggctag agaccctgcg acgaatcctt ttgtggccct
     2761 tcgggtggag gagctccccc ctgctccggc gtgtcgggtt tccgtggc
//
LOCUS       DAOSUT010000279         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_17072_length_2480_cov_10.724124, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000279 DAOSUT010000000
VERSION     DAOSUT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17072_length_2480_cov_10.724124"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLD05_15550"
     CDS             complement(<1..632)
                     /locus_tag="PLD05_15550"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOP78900.1"
                     /translation="MSEKTLVEAIAYCDEPTALRLVKEALQSGVPPQEILAQCNQGML
                     ELGNRFSRDECFLPDLMFGGMIMKNIMAELGPHLAKQAAGQAGTSRPKAVIGTVQHDV
                     HDIGKDIVVMMLRGSGFDVVDLGVDVPPARFVEAIRQHQPCVVGMSLLLTTCYSSVTA
                     TVEAIRQAGLRDQVAIMVGGAAASELLAKNAGCDFYGKTAVDGLKFACQVA"
     gene            complement(709..915)
                     /locus_tag="PLD05_15555"
     CDS             complement(709..915)
                     /locus_tag="PLD05_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78901.1"
                     /translation="MTTQPEKTNPSSMDKLGVSIRPPGTCIPWEEKRRELGEIKGDEE
                     LVKRIWENIDALGYTYIWQVLLSF"
     gene            complement(1149..2345)
                     /locus_tag="PLD05_15560"
     CDS             complement(1149..2345)
                     /locus_tag="PLD05_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOP78902.1"
                     /translation="MVDAVEQRYQERLKRYVTAMRKGKPDRIPIRPFAAEFTAVYAGY
                     TIQEVTHDYQKAFDAVIRCATDFDWDAVVPNMVYVWTGLVQAINLRYYAVPGTDLPPD
                     VCFQYLEPPEENAWMRPDEYDALIEDPTGFLYNVWLPRVSRDVVPPGQPNTFRNNMSF
                     VLGGMAMMDYFMALGRQIERLRRETGTVSAISGILKAPFDILADKLRGYKGLCMDLYR
                     QPDKVLKACEALAPHLLHVAKSGADPAKNVPIGLWMHRGCIPFLSPQQFERFYWPTLK
                     WIIESLWADGHQVLFYAEGEWNPNLKYIAQLPDLSIIYHVDRGDIFEVHRTVGHKFCI
                     SGGIPNDLLAFGTPQDVKEYCKKVIDGVARDGGYIMDASAIMQNDTKPENVKAMTEFT
                     REYGVY"
BASE COUNT          523 a          748 c          663 g          546 t
ORIGIN      
        1 gcgacttggc aggcgaattt caatccatcg acggccgtct tcccgtagaa atcacagccc
       61 gcgttcttgg ctagcagttc actggcggct gccccgccca ccatgatcgc cacctggtct
      121 cgcaaaccag cttggcggat ggcctccacc gtggccgtca cggatgaata gcacgttgtc
      181 aaaaggagac tcatccccac gacacaaggc tgatgttggc gaatggcctc cacaaatcgg
      241 gccggcggga catcgacgcc taagtccacc acatcaaagc ccgaaccacg aagcatcatc
      301 accacgatgt ctttaccgat gtcatgcacg tcgtgctgga ccgtaccgat gacggccttg
      361 ggcctgcttg tgccggcttg accagctgcc tgttttgcca gatggggccc caactccgcc
      421 atgatgttct tcatgatcat gccgccgaac atgaggtccg gtaggaagca ctcatcgcgg
      481 gaaaagcggt tgcccagctc cagcattccc tgattgcact gggctaggat ctcttgcggc
      541 gggacacccg attgcagcgc ctccttgacc aagcgcaagg cggtcggttc atcacaatac
      601 gcaatggcct cgacgagcgt cttttcactc ataatcttcc cccgagaacg tccgcaacgc
      661 cggccttttc ccctcgactg gcgtcgccga tgcaaatctg gccaaatgtc aaaacgatag
      721 caaaacctgc caaatatacg tatagcctaa cgcatcgatg ttttcccaga tgcgtttcac
      781 aagttcctcg tcacccttga tttcgccgag ttcgcggcgt ttttcctccc aagggatgca
      841 cgtcccggga ggccgaatcg acacgccaag tttatccatg ctcgaagggt tcgtcttttc
      901 cggttgggtc gtcataaccg aactccactc tttcgtgatc ggtcccaaac gccgtctgac
      961 cgcgaaattt taagttcctt ttaagagccc atcccatact ctgccctcgc ccgttgggcg
     1021 ggagaggagt cgggggtgag ggtagctcga attgctgccg gttttctcga tcgacgccgg
     1081 cgatggcttg ccctcacccc aaccctctcc cgaggggaga ggggagactt tttgcgatag
     1141 agtctaaatc aataaactcc gtattcccgt gtgaattctg tcatggcctt gacgttttct
     1201 ggcttggtgt cgttttgcat gatggcgctg gcgtccatga tgtagccgcc gtcgcgggcc
     1261 acgccgtcaa tgaccttctt gcagtactcc ttcacatctt gcggtgtccc aaaggccaat
     1321 aggtcgttgg gaatgccgcc gctgatgcag aacttgtgtc ccaccgtgcg atggacctca
     1381 aagatatcgc cacgatcgac atgatagatg atgctaagat cgggaagttg ggcgatgtat
     1441 ttcaggttgg ggttccactc gccttctgcg taaaacagga cttgatgccc atccgcccaa
     1501 agcgattcga tgatccattt caacgtcggc caataaaacc gctcaaattg ctgcggcgag
     1561 aggaaaggaa tgcatccgcg gtgcatccac aggccgatag gcacattctt cgccggatcg
     1621 gcccccgact tggccacgtg cagtaagtga ggggccaggg cctcgcaggc cttgagaacc
     1681 ttatccggct gccggtacag gtccatgcac agacctttat atccacgcag tttatcggcc
     1741 agaatgtcga aaggtgcctt gaggatcccc gaaatcgccg acaccgtccc cgtctcacgt
     1801 cgcagccgct cgatctgccg tcccagggcc atgaagtaat ccatcatggc catgccccca
     1861 aggacgaacg acatattgtt gcggaaggtg ttaggctgac cgggaggaac gacatcgcgg
     1921 ctgacccgcg ggagccacac gttatagagg aatcccgtcg ggtcttcgat gagggcatcg
     1981 tattcgtctg gacgcatcca ggcgttttct tccggtggtt ccagatattg aaaacagaca
     2041 tctgggggca ggtcggtgcc ggggactgcg taatatcgga gattgatggc ctggaccaaa
     2101 cctgtccaga cgtaaaccat attgggcacc acggcgtccc aatcgaagtc ggtggcacaa
     2161 cggattacgg catcaaaggc cttttgatag tcgtgggtca cctcttgaat tgtgtaaccc
     2221 gcataaaccg ccgtgaactc cgcagcgaat gggcgaatcg gaatgcggtc gggcttgccc
     2281 tttcgcatgg ccgtcacata ccgtttgagc cgttcctgat accgttgctc cacggcatcg
     2341 accatggtga cccctcctcg agcgcattag ttggaaatta gcatcgtcgg gcgccgcatc
     2401 cccgaaaccc ctccgaggac acgcccggat gatggcctcg ccataatcaa tgtcgcccag
     2461 tatgccataa ccttccccgg
//
LOCUS       DAOSUT010000280         2443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_17430_length_2443_cov_10.775963, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000280 DAOSUT010000000
VERSION     DAOSUT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_2443_cov_10.775963"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /gene="glgB"
                     /locus_tag="PLD05_15565"
     CDS             complement(<1..1997)
                     /gene="glgB"
                     /locus_tag="PLD05_15565"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOP78903.1"
                     /translation="MRRIHPAGLYEAVCPLNGQRPEYLLKVADQDGKQVIMHDPYAFP
                     PLLTEYDLYLLNEGRHWRSYNKLGAQFRVVNGVEGVNFAVWAPNATSVSVIGDFNRWD
                     GRCHPMRKHIPSGFWELFIPGLKEGDLYKFQIRFHDQVFEKADPFAFYAEVPPRSASR
                     VFNINRYQWQDREWMEQRPKRNWLEEPIAIYEVHLGSWRRSPNGHNGWMNYRELAPLL
                     VDYVKKMGYTHIELLPVAEHPLTTSWGYQVIGYDAPTSRYGTPEDFQYFVDYCHQHGI
                     GVIIDWVPAHFPKDGHGLRRFDGTALYEHEDPRLGEHRDWGTHIFNYGRYEVRNFLIA
                     NALFWFDKYHIDGIRVDAVASMLYLDYSRGPGEWIPNEFGGRENLQAIAFLKLLNEQV
                     HLQFPGALTIAEESTAWPGVSRPTYQGGLGFSMKWNMGWMNDTRTYMRKDPIYRKYHH
                     DQLTFSLIYAFHENFVLPLSHDEVVHGKGSLIDQMPGDWWQKFANLRLLYSYMWTHPG
                     KKLLFMGSDFGQWHEWNCDGELQWELLDWPTHRGLQQCVADLNHLLRREKALHEVDFN
                     WEGFQWIDCHNWEESTLAYIRRAKDPSDYLVIVCNFTPVPRWEHRIGVPEVGRFDEIF
                     NSDAQLYGGGNIGNCGGVVSEPIPWHGFPASMKIVLPPLAAV"
BASE COUNT          580 a          725 c          612 g          526 t
ORIGIN      
        1 acggccgcca aggggggcag gacaatcttc atcgacgccg ggaacccgtg ccaggggatc
       61 ggctcggata ccaccccacc gcagttgccg atatttccac cgccatagag ctgggcatcg
      121 ctgttgaaga tttcgtcaaa acggccgacc tcagggacac cgatccggtg ctcccatcgc
      181 gggaccggcg tgaaattaca aacgatgacc aggtaatcgg aagggtcttt cgcccgacgg
      241 atatatgcca gggtgctctc ttcccaattg tggcagtcaa tccactggaa gccttcccaa
      301 ttgaaatcga cctcgtgcag ggccttttct cggcgtaaga ggtgattcag atccgccacg
      361 cactgttgca ggccacgatg cgtcggccaa tccagcagtt cccactgaag ttctccgtca
      421 caattccact catgccactg gccaaaatcg ctgcccataa aaaggagctt tttgccggga
      481 tgcgtccaca tgtagctgta cagcaagcgg aggttggcaa acttctgcca ccagtccccc
      541 ggcatctgat cgattaacga gcctttcccg tggacaacct catcatgcga gagtggcaag
      601 acgaaattct cgtgaaaggc gtagattagg ctgaatgtta actgatcgtg atgatacttg
      661 cggtagatgg ggtccttccg catgtaggtg cgggtatcgt tcatccagcc catgttccac
      721 ttcatgctga agcccaaccc gccctgataa gtaggccgcg aaacgcccgg ccaagccgtg
      781 gattcctcgg cgatcgttaa ggcccccgga aattgcagat ggacctgttc gttgagcaac
      841 ttcagaaagg cgatggcctg gagattttct cgcccaccga attcattggg aatccactcc
      901 ccgggtccac ggctgtagtc caaatacagc atcgaggcca cggcatcaac ccggataccg
      961 tcgatgtggt acttgtcaaa ccaaaacaag gcgttggcaa tgaggaagtt tctcacctca
     1021 taacgaccat aattgaagat gtgagttccc caatcccgat gttcgcctaa tcgggggtct
     1081 tcgtgctcat aaagggccgt gccatcaaaa cgccgcaagc cgtgcccatc cttgggaaaa
     1141 tgcgcaggaa cccagtcgat gatcacacca atcccgtgtt ggtggcagta atcgacaaag
     1201 tattggaagt cctcaggcgt cccgtagcga gaagtgggcg catcgtagcc gatcacctga
     1261 taaccccaac tggttgtcag gggatgctcg gcgacgggca gcagctcgat gtgcgtgtag
     1321 cccatcttct tgacgtaatc gaccaagagg ggggcgagct cacgataatt catccaaccg
     1381 ttgtggccat taggcgagcg ccgccaactc cccaggtgga cctcataaat cgcgatgggt
     1441 tcttccaacc agttccgctt gggccgctgt tccatccact ctcggtcttg ccactgatac
     1501 cggttgatat tgaaaacgcg tgaggcacta cgtggcggaa cctcggcgta aaaagcaaaa
     1561 ggatcggcct tctcgaacac ctgatcgtgg aagcggatct ggaacttata gagatcgcct
     1621 tctttcaagc cgggaataaa cagttcccaa aagccgctgg gaatgtgttt ccgcatgggg
     1681 tggcaacgac cgtcccagcg attgaagtct ccgatgacac tgacacttgt cgcattgggt
     1741 gcccaaaccg caaaattgac gccctccacc ccattgacca cacgaaactg agcgccaagc
     1801 ttgttataag accgccagtg ccgcccttca ttgagcagat acaggtcata ctcggtcagc
     1861 aaaggaggaa acgcatacgg atcgtgcata atgacctgct tgccgtcttg atcagcgact
     1921 ttcaataaat actcgggccg ttggccgttg aggggacata cggcctcata aagtcccgcc
     1981 gggtgaatcc gtcgcatcgg ccgcggaaca ccctcatgcg ccagatcaat gacccaggcc
     2041 tgctgcgaat ggggcaaaaa cgcccgaatc gccaagaccc gccggtcacc atcttccacc
     2101 acatgaggac ccagaacttc aaagggattc tcgtgttgac cctccaacag tcggccaagt
     2161 tgctctaacg gaacgctcgt ccgcacagaa acctccttgt cacccagatg attctaccct
     2221 aatagtttac ccaaacctca aggatctctc atgctcccaa taaacaagga cgcccctttt
     2281 tgtctaatcg tctgcatcgc agctattttc aatagtttgc cccagttgtc cgcattatca
     2341 gtcgcttccg catcagtcgt tccaaacgat cgatccgacc ttatgtccca gccgcagtct
     2401 atcccggatg ctttttttcg agcctatccc ataatcgctc ctc
//
LOCUS       DAOSUT010000281         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_17699_length_2413_cov_7.464377, whole genome shotgun sequence.
ACCESSION   DAOSUT010000281 DAOSUT010000000
VERSION     DAOSUT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_2413_cov_7.464377"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLD05_15570"
     CDS             complement(<1..514)
                     /locus_tag="PLD05_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOP78904.1"
                     /translation="MAYLSSRRSFLKRVSAAATCLILPSSRIAFSYEANEKVHYAAIG
                     LGKRGSYLAPTFARIGGELVVLCDADQERIGPFAQKFPKGSVYRDFRRMFDECEKSID
                     AVVIATPDHTHAVISAAAIRRGKHVYCEKPIAHDVREARVLRELAAQKKVATQMGNQG
                     MATDSFRRTLE"
     gene            826..>2413
                     /locus_tag="PLD05_15575"
     CDS             826..>2413
                     /locus_tag="PLD05_15575"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOP78905.1"
                     /translation="MPEHAVFVVGFGFAFLFLGYLLYGRFLGKRVFHLKGDAPTPAHT
                     MYDGVDYCPAPTPVLFGHHFASIAGLGPILGPALAVIWGWFPAVVWVLVGAVFIGAVH
                     DLGALVVSLRHRGRSVGEVAHYVLGPRARLLALLIIFFLMSLAMGAFCNTLADLFLNF
                     NPDAIVPSVGLMILAVLFGLSVYRLKAPLALATIVALLIFAGLIYLGEKVPVLSYQWL
                     CSAETQQAINQAKDATAPSGGPTFARPYGAKLAIDYFRATGREDIVHELGAIDKPESV
                     LGKMNYAWIGALLCYAFLASVLPVWLLLQPRDYLNSFQLYFALITLFLGLIVATLGGS
                     EFARIDAPLIRTQVPGAPPMIPLLFVTIACGAVSGFHSLVSSGTTVKQLDREKDAVLI
                     GYGAMLVEGALALLVIAACTAGLSAVDWGKDGVYSSWTAMRGLAAQLNAVVRGSANIL
                     SHLGIPVAIGQTFIAVTVTAFALTTLDSATRLLRFNVEELFNAVGLRFLANRYVGSLL
                     AVGGIGFFALVPAGKALWTLF"
BASE COUNT          450 a          656 c          663 g          644 t
ORIGIN      
        1 gttccaaggt cctgcggaat gagtccgtcg ccattccctg attgcccatc tgcgtggcaa
       61 cctttttctg tgcggccaat tctctgagga cacgtgcctc gcggacatcg tgggcaatcg
      121 gcttttcgca atagacgtgc tttccccggc ggatcgccgc cgccgaaata acggcatgtg
      181 tgtggtctgg cgtggcgatc accacagcat caatgctctt ctcacactca tcgaacatgc
      241 ggcggaaatc tcgataaact gatccctttg ggaacttctg cgcaaagggc ccgattcttt
      301 cctgatctgc gtcgcaaagc acgaccaatt cacctccaat cctggcaaat gtcggcgcca
      361 ggtaacttcc ccttttcccg agtccaatgg ctgcgtagtg aaccttttcg ttggcttcgt
      421 aggaaaaagc tatcctggac gacggcaaga ttaggcaagt ggcggcagcc gacacacgct
      481 ttaggaagct ccgacgactc gacaggtagg ccatggtcga tactccttgg gttaggtaat
      541 tccgaaaggg agggtcaacc caacgaacaa tgccgcatct gcaaataatg ttgtaccgtc
      601 ttgaccgaga atgtccactg cgcgagagtg catctgccat gacctgcacc catgaccaac
      661 tggttaggta tgagaaagtg ggggagcgtt aggttgtgat tgaaagaaat tcagcaaaaa
      721 actatgctgg atgcagaagt taaaaacgaa gtttcccgag aacaccgttt ctgattcgga
      781 tgggttacag gacactcggt tttcctccga tcacgaggcc ccgctatgcc tgaacatgcc
      841 gtgtttgttg tcgggttcgg ttttgccttc ctgtttttgg gatatctcct ttatggacgg
      901 ttcttgggca agagagtttt tcacctgaag ggagatgctc cgacgcccgc acatacgatg
      961 tacgacggcg ttgactactg ccccgcgccg acgcctgtgc ttttcggtca tcattttgcc
     1021 tccatcgccg gattagggcc tattttagga ccggcgttgg ccgtcatttg gggttggttt
     1081 cccgcagtcg tctgggtttt ggtcggcgcc gtctttatcg gagcggttca cgatctgggg
     1141 gcgctggtcg tgagtttgcg tcaccgtggg cgatccgtgg gagaagtcgc gcattatgtg
     1201 cttggtccgc gggcgcggct tttggcacta ctcattatct tctttctcat gtcgctggcg
     1261 atgggtgctt tttgcaatac gttggctgac ctcttcctta acttcaatcc tgacgcgatt
     1321 gtgccttctg tcggtctcat gatccttgct gttctttttg gcctatcggt ctatcgattg
     1381 aaggcccctt tggcgctggc aacgatcgtt gcgctgctga tcttcgcggg attgatctat
     1441 ctcggcgaaa aggtccccgt gctcagctac caatggttat gttccgctga gacacaacaa
     1501 gcaatcaacc aagccaaaga tgcaaccgct ccatcgggcg gtcccacttt tgcgcggccc
     1561 tacggtgcaa agttggccat tgattacttc cgcgctacgg gacgcgagga catcgtccat
     1621 gaactgggcg ccattgataa gccggaaagt gttcttggca agatgaatta tgcctggatc
     1681 ggcgcgcttt tgtgctatgc ttttttggcc tcagtccttc cggtgtggct gttactccaa
     1741 ccaagggatt acctcaatag cttccagctc tattttgccc tcatcacgct cttcctaggg
     1801 ctaatcgttg caactctggg gggcagcgag tttgcccgca tcgacgcccc gcttatccgc
     1861 acgcaagtgc cgggtgcacc gcccatgatc cccttgctct tcgtcactat tgcctgtggg
     1921 gccgtgagcg gcttccattc tcttgtttcc agcggaacga cggtcaaaca gcttgatcga
     1981 gaaaaggatg ccgtgctcat cggctacggg gccatgctcg tagagggggc tttggccctc
     2041 ctggtcattg cggcctgcac ggctggccta agcgctgtcg attggggtaa ggatggtgtc
     2101 tattcgtcat ggacggccat gcgaggtctg gccgctcaat taaacgctgt tgtccgggga
     2161 agtgctaata tcctttctca tctcggcatt cccgtagcca ttggacaaac atttatcgct
     2221 gtcacggtga cggcttttgc cctcacgacg ctcgattcgg caactcggtt gctgcgattc
     2281 aatgtagaag agctattcaa tgcggtcggc ctacgtttct tggcaaaccg ctatgttggt
     2341 tcactgttgg ccgtgggtgg catcgggttc ttcgccttgg ttccggccgg caaagctctg
     2401 tggacactct ttg
//
LOCUS       DAOSUT010000282         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_17725_length_2410_cov_7.601274, whole genome shotgun sequence.
ACCESSION   DAOSUT010000282 DAOSUT010000000
VERSION     DAOSUT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17725_length_2410_cov_7.601274"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLD05_15580"
     CDS             <1..1240
                     /locus_tag="PLD05_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOP78906.1"
                     /translation="SSVLDPHKVQDTQFTAKLVNGRVIQPQRELPLLAQCEVLVVGGG
                     PAGVSAAIAARRAGARVVLVERYGHFGGLWTGGLVLLVLGHIVIGGKQVCMGIGEEMM
                     QRLEKLPGGIINRQPGKNPTVDAEALKYVMVEMIEEAGVEVFLHAWGVDAILEGNKVR
                     GAVFESKSGRQAILADVVVDATGDGDIFAAAGAEFEHRTHNVGLVSRVGNLDRVDPSR
                     VPPEKRPKRLGGPTPAPGINWVNLTGPEVDGLDVRTLTRMEMSHRKFIWRNVQQARQV
                     PGYESLFLVETAPQLGVRITRVLNGLATVRYADLKAGRRFEDVVAVGGSTNGQHGEWQ
                     VPYGALVPKTVDNVLAAGRCISAEMKMADLVRLIPNCFATGHAAGVAAAVAVQDRCAP
                     RHVDVPKVQKILREQGAYLG"
     gene            1233..>2410
                     /locus_tag="PLD05_15585"
     CDS             1233..>2410
                     /locus_tag="PLD05_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOP78907.1"
                     /translation="MAEVHREKKAKPGIASAQQDSWTLSGPGPSMKFFLSSSDTWFRV
                     ARWAIRLAVLIGVVGLLFSKASRDPRLATLPAGLSPSIVLCGWIAAGTFSAIGWLALG
                     IACVAAFRLRWFCRWMCPLGTCNELSTLLGKRVGLPSPSVPHLGHYLIFATLGGAMMG
                     FPILLWLDPLALFSSLGQPLPLQSLPFLVGISGLVLAMGVSFLLPGTWCLRICPLGAL
                     QEVLFVFRRGIIRTLVRLFRVGQASPSREMPRFDGPELSELLRISRRGVIGVVGAIAW
                     TYLARPSALSKTLPLRPPGAWPDERRFLALCVRCGNCMRVCPTGIIRPQQSTDGVAMW
                     LTPRLVFTDDFCEPSCNRCAEVCPSGALTPFSKAEKPHVVIGKAVVELDECLLTVDRE
                     "
BASE COUNT          419 a          662 c          761 g          568 t
ORIGIN      
        1 cagcagtgtg ctcgacccgc acaaggttca ggacacgcaa ttcaccgcaa agctggtcaa
       61 tggtcgtgtc attcaaccac aacgggaatt acctctgcta gctcagtgtg aagtactggt
      121 cgtcggcggt ggacccgccg gggtctctgc cgctatcgcg gcgcgacgtg cgggtgcccg
      181 tgtcgtcctg gtagaacgtt atggacattt cggcggccta tggaccggcg gattggtgct
      241 tctcgtcttg ggacatattg tcataggcgg gaagcaagta tgcatgggta ttggcgagga
      301 gatgatgcag cgcctggaaa aattgccggg aggtattatc aatcgtcagc ccgggaagaa
      361 ccccaccgtt gatgccgagg cactgaagta cgtgatggtc gagatgatcg aggaggcggg
      421 cgtcgaagtc tttttgcacg cctggggtgt cgatgccatt ttggaaggga acaaagtccg
      481 gggggctgtc ttcgaaagca agtcgggacg acaggccatt cttgccgacg tcgtggtaga
      541 tgcgacgggc gacggggata tcttcgccgc ggcaggggcg gagttcgaac accgtacgca
      601 caacgtgggg ctggtgtcga gggtgggcaa tctcgatcga gtcgatccct cgcgggtccc
      661 gccggaaaag cgtcccaaac gattgggcgg tcctacgccc gctccaggaa tcaattgggt
      721 caatctaaca gggccggaag tggatgggct tgacgtccgc accctgaccc gcatggaaat
      781 gtctcaccga aagttcatct ggcggaatgt gcaacaggcc cggcaagtgc ccggttatga
      841 gtcgctcttt ttggtcgaaa ccgccccgca attgggtgtt cggatcaccc gggtcttgaa
      901 cggtctggcc accgttcgct atgccgattt gaaggccggt cggcgtttcg aggatgtcgt
      961 agcggttggt ggctcaacaa atggtcagca cggcgaatgg caggtgccgt atggtgcttt
     1021 ggttcccaaa acggtggaca atgtcctggc agccgggcga tgcatcagtg ccgagatgaa
     1081 aatggccgat ctggtgcggt tgatccccaa ctgttttgcc acgggccacg ccgcgggagt
     1141 cgccgccgcc gtggcagttc aagatcgttg tgcccctcgc catgtggacg tgcccaaggt
     1201 tcaaaagatt cttcgtgaac agggggctta ccttggctga ggtccatcgc gagaagaagg
     1261 ctaagccagg tattgcgtct gcccagcagg attcctggac tttgtcgggc cccggaccta
     1321 gcatgaagtt tttcctcagc tcttcggaca catggttccg tgttgcccgc tgggcgatcc
     1381 gtcttgccgt tttgatcggt gttgtcggcc ttctcttcag caaagcgtcg cgtgatccga
     1441 ggctcgcgac cctgcccgcc gggctaagcc ccagcattgt gctttgtgga tggatcgcgg
     1501 cgggaacttt ctcggctatc ggatggctgg cgcttggcat cgcttgtgtg gcggcttttc
     1561 gacttcgttg gttttgccgt tggatgtgcc ctctggggac atgcaacgaa ctatccaccc
     1621 tgttgggaaa aagggtaggc ttgccaagtc cttccgtacc acacctcgga cactatttga
     1681 ttttcgctac cctcggtggg gcaatgatgg gcttccccat tctgctgtgg cttgatcccc
     1741 tggccctgtt cagcagttta gggcaaccat tgcctctcca gtcgcttccg tttcttgtag
     1801 gaatcagcgg acttgtgctg gccatgggag tgagcttctt actgccggga acgtggtgcc
     1861 ttcgcatctg cccgttaggg gccctccaag aagtcttgtt tgtcttccgg cggggtatca
     1921 ttcgcacgct cgtcaggctt ttcagggtcg ggcaggcaag tccatcgcgt gagatgcccc
     1981 gcttcgatgg accagagctg tctgagttgc ttcgcattag ccggcgcggt gtcattggcg
     2041 tggtgggggc catcgcatgg acctacctgg ctcggccctc ggcattgtcc aagacactcc
     2101 cgcttcgtcc ccccggggca tggccggacg agcgacggtt tcttgccctg tgtgtccggt
     2161 gcggcaactg tatgcgcgtc tgtcctaccg gcatcattcg tccccaacaa tcaaccgacg
     2221 gtgtggcaat gtggctcacg ccccggctcg tatttaccga cgacttttgt gagccttcct
     2281 gcaaccgctg tgctgaggtg tgtccttccg gtgcgttaac accgttttcc aaggctgaga
     2341 agcctcacgt ggtcatcggc aaagcggtgg tcgaactcga tgagtgtctt ttgactgtgg
     2401 accgcgaatg
//
LOCUS       DAOSUT010000283         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_17988_length_2385_cov_8.016309, whole genome shotgun sequence.
ACCESSION   DAOSUT010000283 DAOSUT010000000
VERSION     DAOSUT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17988_length_2385_cov_8.016309"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLD05_15590"
     CDS             <1..528
                     /locus_tag="PLD05_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78908.1"
                     /translation="GSPLPWTGDYFDGLGNRITMFVYANPELDTIGELRKAQRDPSVN
                     RGDGYALVRFRKSDRTITFECWPRFADVSKGHAGQYPGWPITIKMFDNDGRKPVGYLP
                     ELRFQNIADPVVQVVDESNGEILYTRRIMGHRFRPPVFSPGSFTIKVGRDRPERIVAE
                     HVKTTESEAIISVGF"
     gene            651..1334
                     /locus_tag="PLD05_15595"
     CDS             651..1334
                     /locus_tag="PLD05_15595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOP78909.1"
                     /translation="MCQEPTRLPLIGDRAPEFRAKTTQGEINFPKDFEGSWVVFFSHP
                     ADFTPVCTTEFMTFAAMQEEFAKRNCKLLGLSIDSIYAHIAWLRTIYEKIEYKGMRNI
                     EITFPVIEDLKMEVSRLYGMLQPGASTTQAVRAVFVIDPKGIVRAILYYPLSNGRDIN
                     EILRLLIALQTSDEYGVATPANWRPGEDVIVPPPGSCGVAKERVTKPDPDVKCLDWFM
                     CLKKCPPIK"
     gene            1472..2335
                     /locus_tag="PLD05_15600"
     CDS             1472..2335
                     /locus_tag="PLD05_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOP78910.1"
                     /translation="MDTANARITVLVENTAKQPKLLAEHGLAYWIEYGGHHFLFDTGQ
                     GYVLRHNAAELGIPLSLVEAVVLSHGHYDHTGGVGQVLCGDHPITVYAHPAAFQPKFA
                     RHSGVRPIGIPPSATEALRRPNIVVRPVTQPTMIVPGVMATGPIPRTSEMEKFNPRFF
                     LDERCQTIDPLEDDQALFLTTSKGVVILLGCAHSGVINTVRYVEKLLPDQPICALIGG
                     MHLVEASEEQIQWTIKELKDREIPLIAPAHCTGIKATVALWQAFADHCTEACVGKQWQ
                     FDLAVRSEGNR"
BASE COUNT          525 a          638 c          649 g          573 t
ORIGIN      
        1 ggcagtccct tgccttggac gggggattac tttgacggtc tgggaaaccg cattacaatg
       61 ttcgtttacg ccaatccgga actcgacaca atcggtgaac ttcggaaggc ccaacgggac
      121 ccgtcggtga atcgcggtga tggttatgcg ctcgttcgtt tccgcaagtc ggatcgaacg
      181 atcacctttg aatgctggcc tcgctttgcc gatgtctcca agggtcatgc cggccaatat
      241 ccaggttggc caattaccat caagatgttt gacaatgatg gccgaaagcc cgtcggctat
      301 ctgcccgagt tgcggtttca aaacatcgct gatccggtgg ttcaggtcgt cgatgagtcc
      361 aacggtgaga tactttatac tcggcggatt atgggccatc gttttagacc acctgtgttt
      421 tcgccggggt cgttcaccat caaagttggg cgggatcgtc ccgagcgtat tgtggccgag
      481 catgttaaga cgacggaaag tgaggcaatt atctccgtcg gattttgaac atgagagaac
      541 gcaggcaaga ggtttgtttt gatcggaagg cgtgtttcgg cggcttaggt tgaattgaac
      601 tgtgcgtcag tgctgaggta acaatttacg aaaaaaggag gacgtcaacg atgtgtcaag
      661 aaccgacccg tttaccgtta attggtgatc gcgccccgga attccgcgcg aaaacaactc
      721 agggggaaat caactttccc aaggattttg aggggagttg ggtcgttttc ttttcccatc
      781 ctgccgattt tacgcccgtt tgtactaccg aattcatgac gtttgccgcg atgcaagaag
      841 agtttgccaa acggaactgc aagctcttag ggctttccat cgacagcatt tacgctcata
      901 tcgcctggct gcggacgatt tacgaaaaaa tcgagtacaa ggggatgaga aatattgaaa
      961 tcaccttccc ggttattgaa gacttgaaga tggaagtctc cagactctac ggaatgcttc
     1021 agcccggagc gagcacgaca caagctgtgc gggccgtttt tgtcatcgac cccaagggta
     1081 tcgtgcgtgc catcctctat tatccgctgt caaacggtcg cgacatcaat gaaatccttc
     1141 gcctgctgat agctcttcag acatctgacg agtatggtgt ggccacaccc gccaactggc
     1201 gtccgggcga ggacgtgatt gtaccgcccc ccggctcctg tggcgtggcc aaggaacgcg
     1261 tgacaaaacc cgatcccgac gtcaagtgtc tcgattggtt catgtgcttg aaaaagtgtc
     1321 caccaatcaa ataacacgga ttttttgtca ccaggggttg cctccttcgg caccgtgacg
     1381 cgccacacgt agtgacggtt cgggtacccg gtggagtgct ccggtgaccg ttctttcgtt
     1441 ttccctcgcg atcagacaag tagcgagaac aatggatact gcgaacgcac gtattaccgt
     1501 cctggtcgag aatacggcca agcaaccaaa gctcctggca gagcacggac tggcgtattg
     1561 gatcgagtac ggcggtcatc attttctctt tgataccggg cagggctatg tccttcggca
     1621 taacgccgct gagctaggta tcccgctttc gctggttgag gccgttgtcc ttagccacgg
     1681 tcactacgat catacgggcg gggttggcca agttctttgt ggtgatcatc ccatcaccgt
     1741 ttacgcacac ccggcggcat tccagcccaa gtttgcccgt cacagcgggg tccgcccaat
     1801 aggaattccg ccaagcgcta ccgaggcgct ccggcgaccc aacatcgtgg tgcgccccgt
     1861 cacgcagcct acgatgattg ttccgggggt catggctacc ggtcccatcc cccgcacgag
     1921 cgaaatggag aaattcaatc cccgcttctt cctggatgaa cgttgccaaa caatagaccc
     1981 cttggaagac gatcaggccc tattcttgac cacatcgaag ggggttgtca tccttttagg
     2041 ctgcgctcac tcgggtgtca tcaataccgt tcgttacgtg gagaagctcc tgcccgatca
     2101 accgatttgc gccctcatcg ggggcatgca tttggttgaa gcctctgaag agcaaatcca
     2161 atggacaatc aaggagctga aagatcgcga aatcccgctc attgccccgg cccattgcac
     2221 gggaatcaaa gcgacggttg cgctgtggca ggcctttgcc gatcactgta cggaagcctg
     2281 tgtcggtaag cagtggcagt ttgatttggc agtgcgttcg gaaggtaacc ggtaatctca
     2341 tccttgtcgt tttttctcca cggcctccat gcgttcaaca tgaat
//
LOCUS       DAOSUT010000284         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_18925_length_2299_cov_8.998663, whole genome shotgun sequence.
ACCESSION   DAOSUT010000284 DAOSUT010000000
VERSION     DAOSUT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_2299_cov_8.998663"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLD05_15605"
     CDS             complement(<1..870)
                     /locus_tag="PLD05_15605"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOP78911.1"
                     /translation="MPILGAHQSIAGGFEKAVERAVATGCECLQIFTKSCTRWKSPPL
                     SEEQIAAFQRALQRSGLRHPVGHDGYLINLASPDPKLWRRSLESLEDEYRRATQLGLE
                     ALIIHPGASIHNDTDAALRRIATALREVLNRNPESKTWILLETLAGQGSQVGRSFDEL
                     ATIFELMDDTQRLGVCWDTCHMLAADYPIDSPAGWDRTVRWFERQIGLDRLKAIHLND
                     SKTARGSGVDRHEHIGLGYVGRETFRRILQDPRVNHLPMYIETPKGKASDGRDWDVIN
                     LTLLRELAAERQDL"
     gene            complement(938..1939)
                     /locus_tag="PLD05_15610"
     CDS             complement(938..1939)
                     /locus_tag="PLD05_15610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOP78912.1"
                     /translation="MHEFGERVFHVLPRQVNMTVAAALREWLPGVSWSRIKKLIEGRY
                     IQVSGNLCLDPARRLKLQDVVKLLPRPGQAPPSPRDVAVVYLDEHIVVVDKPAGMTTN
                     RHREELDASARRRQLQPTLDEVLPHIIARMEGKKKRHKGVPPPVRAVHRLDRETSGLI
                     VFARTSEAERGLMEQFRKHTTTRRYLAIIPGYMEAQTIQSYLVRDRGDGRRGSTKKPG
                     EGRLAITHVKPVERIGDYTLVECRLETGRTHQIRIHLAEAGHPVCGEKLYNRPLFGKP
                     IEDNSGAPRLCLAAVELGFIHPVTGEPMNFEIPLPRDLMDFVYELRRRARLCQKKES"
BASE COUNT          480 a          653 c          661 g          505 t
ORIGIN      
        1 tagatcctga cgctcggcgg ccagttcacg caggagtgtc aaattgataa catcccaatc
       61 tcggccatca gaggctttcc cttttggggt ctcgatatac atgggcagat gattcacccg
      121 ggggtcctgc aaaatccgcc ggaaggtctc gcggcccaca tagcccaagc cgatgtgctc
      181 gtggcgatca acaccactgc cacgggctgt tttgctgtcg ttgagatgga tggccttgag
      241 gcgatccagt ccgatctgtc gctcgaacca acggaccgta cggtcccaac ctgcgggcga
      301 atcgatcggg taatctgccg ccaacatgtg gcacgtgtcc cagcacactc ctagtcgctg
      361 ggtgtcatcc atcagctcaa agatggtggc cagttcatca aaagaacggc ccacttggct
      421 tccctgacct gccagcgttt ccagaagtat ccacgtcttt gactccgggt tcctgttgag
      481 aacttcccgc agggcagtgg cgatgcggcg gagggcagca tcagtgtcat tatgaatgga
      541 cgcaccggga tggatgataa gggcctctaa gcccaattgc gttgcccgcc ggtattcgtc
      601 ctcgagcgat tccagcgacc tgcgccacag cttggggtcg ggacttgcca aattgatcaa
      661 atagccgtcg tgtcctacag gatgccgcaa gccactgcgt tgtagtgcgc gttgaaaggc
      721 agctatttgt tcttccgaaa ggggtggcga tttccatcgg gtacaacttt tcgtgaagat
      781 ttgcaggcac tcgcaaccgg tcgcgacggc gcgctccacc gctttttcga agccgccggc
      841 aatcgattgg tgagcaccaa gaatcggcat ggctcaactc aagatggcga ggcaactaga
      901 aggggggcca acccaacccc cctcagaccc atgatcgtta cgattccttt ttctggcaca
      961 gtcttgcccg acgccgaagt tcgtacacaa aatccatcaa gtcccggggc aaaggtatct
     1021 cgaagttcat gggttctcct gttacgggat gaatgaatcc caactctacg gcggcaagac
     1081 acagacgcgg tgcgccactg ttgtcctcga tcggcttgcc aaaaaggggc cgattgtaaa
     1141 gcttttcacc acaaacgggg tggccggcct ctgccagatg aatgcggatt tggtgggtcc
     1201 gcccggtttc aagacggcat tccaccaggg tgtaatcgcc gattcgctcg acgggcttga
     1261 cgtgggtgat tgccaaacgg ccctcgcccg gcttcttggt gctgccccgt cgtccgtcac
     1321 cgcgatcacg gacaaggtac gactgaatcg tctgggcttc catgtaaccg gggatgatcg
     1381 ccaaataacg gcgggtggtg gtatgtttgc ggaattgctc catgagaccg cgttctgcct
     1441 cgctggtgcg cgcaaagaca attaagccac tggtttcacg gtccaagcgg tgcacggcac
     1501 gcaccggtgg tggaacgcct ttgtgtcgct tctttttgcc ttccatccgc gcgatgatat
     1561 gcggcaagac ctcgtcgagg gtgggctgga gttgtcgccg tcgagctgag gcgtcgagct
     1621 cctcgcgatg tcgattggtg gtcatccccg ccggcttatc caccaccaca atgtgttcat
     1681 cgaggtaaac cacggcgaca tcccgcgggc tggggggcgc ctgtcctggg cgcggcagga
     1741 gcttgaccac gtcctgaagc ttcagccgcc ttgccggatc aaggcacaaa ttgccactta
     1801 cctggatgta tcggccctcg atgagtttct tgatccgcga ccaggaaaca cccggcaacc
     1861 attcccgcag cgcggcagcc accgtcatgt tgacttgccg cggcaaaaca tgaaagactc
     1921 gttcaccgaa ttcgtgcatg atgattcaat gatctcaact agttcagaca aggtgcaacg
     1981 tctttaggca ggccaatgcc cgattgggga aacctgtgat gtggagtctc tgggaaagaa
     2041 ttctacgcaa taaatctaat gctaaggata acaatttggc gaaaaattga catcccgggg
     2101 cggcatttcc attaagagct tatcccataa tgctcccctt acccgtttgg cgggagaaga
     2161 gtcgggggga gggtggctcg aatcgctgcg gttttctcga tggactccgg cgggcgcttg
     2221 ccctcaccct aaccctctcc cagagggaga gggaactttg ggataggctc tcagacccag
     2281 ggtgtggtgg tcagataag
//
LOCUS       DAOSUT010000285         2220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_19852_length_2220_cov_9.541801, whole genome shotgun sequence.
ACCESSION   DAOSUT010000285 DAOSUT010000000
VERSION     DAOSUT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19852_length_2220_cov_9.541801"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLD05_15615"
     CDS             <1..969
                     /locus_tag="PLD05_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOP78913.1"
                     /translation="MVNAMGIQLPTDDPSNPIPKSPPPDPPLEPQTSPGGLVSVDGRI
                     ILTGNNGRDNDIDIGMSGMLLRFPDGVTSTVNLPWTTIQEAVGESAVTDFLVYDSLGI
                     PIQVRVTMVLESRDSIRTVYRWFADSPDNDPLTGSEISVGTGLIYFDGEGNFISATNN
                     KVSIERRHVSARSPLEFSLDFSKISGLAADRSTLMVSRQDGSAPGVLTSFIVGEDGLI
                     RGVFSNGVTRDLGQIILARFANPAGLVQRGQNLFAEGVNSGLPVIGNPGEQGIGTLVA
                     GAVELSNTDIGGSLIDLILASSMYRGNTRVISTAQQMLDELLSLRR"
     gene            1126..1365
                     /locus_tag="PLD05_15620"
     CDS             1126..1365
                     /locus_tag="PLD05_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOP78914.1"
                     /translation="MIRLTRLSGEPFILNADLICYVEERPDTYITLTTGERVVVRESM
                     EEVLRRAVAYQQAKHLWPSSAPKRVVTDDDQSEKS"
     gene            1369..2151
                     /locus_tag="PLD05_15625"
     CDS             1369..2151
                     /locus_tag="PLD05_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOP78915.1"
                     /translation="MDIATVIGIVGGFLLIVVSIAIAPGASAKAFIDYPSIVMVIGGA
                     IAALLTSMPLKDVLKIVGVVKKALFNKSEDVTAIIQLIVSLAETARRDGLLALENRLT
                     EISDPFIVLGVQMAVDGTRPEVMEDVLRTEMEAVSARHRTGKLIFDQIGKFAPAFGMI
                     GTLVGLIIMLGNMSDPNAIGPGMAVALLTTLYGAIVANVVALPIAEKLGYLSQQEQIR
                     MEIVVRGIMAIQAGENPRVIEQKLRTFVPPKLREQKEDKKAA"
BASE COUNT          498 a          552 c          632 g          538 t
ORIGIN      
        1 atggtcaatg ccatgggcat tcaacttccc accgacgacc catccaatcc cattccgaag
       61 tcccctccgc cagacccacc gctcgagccg caaacgagtc ccggcggctt ggtaagtgtc
      121 gatggccgga tcatcctgac aggcaacaat ggaagggata acgatatcga tattggcatg
      181 tcaggaatgc tcttgcgttt tcccgacggt gtcacgagca ccgttaactt accctggact
      241 accattcaag aagccgtcgg cgagagtgcg gttaccgact tcttggtgta tgactcactt
      301 ggtatcccta tccaagtgcg agtgacaatg gtgctcgaat ctcgcgatag cattcggact
      361 gtctatcgtt ggttcgccga ttcgccggac aatgatccgc tgacaggttc ggaaatttcc
      421 gtcgggaccg ggctgatcta ctttgacgga gagggcaatt ttatctcggc aacgaataat
      481 aaggtttcta tcgaacgacg gcatgtttct gcccgttcgc cgctcgaatt ctcgctcgat
      541 ttctccaaga tctcgggcct tgccgcggat cgcagtacgc ttatggtgtc gcgccaggat
      601 ggttcggctc cgggtgtctt gaccagcttc atcgtgggtg aagacggttt gattaggggt
      661 gttttttcaa acggtgtgac tcgtgatttg ggccaaatca ttctggcacg gtttgccaac
      721 ccggcgggtc tcgtccagcg gggtcagaac ctatttgccg agggtgtcaa ctccggtctc
      781 cccgtaatcg gcaaccctgg tgaacagggg attggaacgc tcgtggccgg tgctgtggag
      841 ttgtcaaaca ccgatattgg cggcagtttg atcgacctaa ttttggcctc cagtatgtac
      901 cgtggaaata cccgggtgat tagcacggct caacagatgc tggatgaact attgtcatta
      961 cgtcgttgat ttcgaatcag cggaagctat gttatcgata agcactgtta tgtgaatcag
     1021 atgtaagatt gacaatgttc ccggtgttaa ctctcgccgg tggggtgggc cttggaccac
     1081 agactgagcg atagtctggg cacagacttg gaccgttcaa cgtccatgat tagattgacg
     1141 cgattgagtg gcgaaccatt tatccttaat gcagatttaa tctgctacgt ggaggaacgt
     1201 cccgatacat acatcaccct aacaaccggg gaacgtgttg ttgtccgtga gtccatggag
     1261 gaggtcttgc ggcgggctgt tgcgtatcag caagcaaagc acctgtggcc gtcatctgct
     1321 cccaaaagag tcgtgaccga tgacgatcaa tcggagaaaa gttgagatat ggatattgcg
     1381 acagtcatcg ggatcgtcgg aggattcctc ctaattgtgg tctccatcgc catcgcgcca
     1441 ggggcctcgg ccaaggcgtt tattgattat ccgtcgatcg tgatggtgat cggaggcgct
     1501 atcgctgcgc tcttgacgtc catgcctctt aaagacgttt tgaagatcgt gggcgttgtg
     1561 aaaaaggcgc tcttcaacaa gagcgaggac gtcacggcga tcattcaact catagtttca
     1621 ttggcggaga cggcccgtcg cgatggactc ctcgcgctgg aaaaccgtct cacagaaata
     1681 tcagatccgt tcatcgtcct gggagtgcaa atggccgtcg acggcactcg acccgaggtt
     1741 atggaagatg tgctccgcac ggagatggaa gctgtatcag ccaggcatcg cacgggcaag
     1801 ctcatctttg atcagattgg gaagtttgcc ccggcctttg gaatgattgg gactttggtg
     1861 ggcttgatca tcatgcttgg taacatgagc gatccgaatg ccattggtcc cgggatggct
     1921 gtcgcacttt tgacaaccct ttacggggcc attgtggcca acgtcgtagc cctacctatc
     1981 gcggagaaat tggggtacct atcacagcaa gaacaaatcc gtatggaaat cgttgtccgt
     2041 gggatcatgg ccattcaggc gggggagaat cctcgtgtga tcgagcaaaa gcttcggacg
     2101 tttgttccac caaagttgcg agagcagaaa gaagacaaga aggcagcgta gcccggtggg
     2161 aaatgcttct cccagcgaga gagtcacgaa atggctggct tgaaagaaga accgtcaggc
//
LOCUS       DAOSUT010000286         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_19876_length_2218_cov_10.554785, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000286 DAOSUT010000000
VERSION     DAOSUT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19876_length_2218_cov_10.554785"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLD05_15630"
     CDS             complement(<1..713)
                     /locus_tag="PLD05_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOP78916.1"
                     /translation="MKLTSTVAANVEKFMEWVVKRNPNEPEFHQAVREVAESIFPFLA
                     EHPIYEKEKILHRITEPERVVMFRVAWVDDKGEVQINRGYRVQMNSAIGPYKGGLRFH
                     PTVNLSVLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDREIMRFCQSFMTELFR
                     HIGPMTDVPAGDIGVGTREIGYLFGQYKRLKVEFSGVLTGKGYGWGGSLIRPEATGYG
                     NVYFVEQMLARRGEGLMGK"
     gene            1284..>2218
                     /locus_tag="PLD05_15635"
     CDS             1284..>2218
                     /locus_tag="PLD05_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOP78917.1"
                     /translation="MPIWGTAQRSCQGDTYPIQSEVTVMRVSKSSSQAGVFRTPYRRE
                     DRKHRHRMEILTVLRKSEQPLSSSDIAQMLAAVGYMLSERSIRLYLAEMCEEGLTISH
                     GHRGHTISEKGLAELRVAQTIQRVGYLSARIDEMTYKMTFDLERRAGTVVVNTSVVNP
                     HDLAVCLDSVCQVFNKGFAMGSRLALLEPGERIGELQIPPGRIGFCTICSITLNSVLL
                     KHGVPTTSRFGGLLEVRDGKPLRFAEIIHYDGTSIDPLEVFIRGRMTDYRGAIATGNG
                     LIGASFREIPEDARDLAQRLSDEMGRIGLGGVMAIG"
BASE COUNT          490 a          613 c          628 g          487 t
ORIGIN      
        1 atcttgccca tgaggccctc gccgcgccgt gccaacattt gttctacgaa atagacgttg
       61 ccataacccg tggcttctgg ccggatgagc gagccacccc agccataacc ttttccagta
      121 aggacgccgc tgaactccac cttgagcctc ttgtactggc caaagagata accgatttcc
      181 cgcgtaccca cgccgatgtc cccggccggg acatccgtca tggggccaat gtgacggaag
      241 agttccgtca tgaagctctg acagaaccgc ataatctcgc ggtcgctctt gcccttggga
      301 tcgaagtcgg aaccgccttt gccgccaccc atgggaagag ttgtcagact gttcttgaag
      361 acctgttcga acgccaggaa tttgaggacg ctcaaattaa ccgtcggatg gaaccgcagg
      421 ccgcccttat acgggccgat ggcgctattc atttgcaccc ggtagccgcg attaatctga
      481 acttcaccct tgtcatcgac ccaggccacg cggaacatca cgacccgttc tggttccgta
      541 atgcggtgaa gaatcttttc cttttcgtag atggggtgct cggcgaggaa gggaaaaatg
      601 ctttccgcga cttcccgaac ggcttgatga aattccggtt cattggggtt cctcttgaca
      661 acccactcca tgaacttctc gacattagcg gcgacggttg aggtgagttt catcgtgtgc
      721 actcctgatc atagaacgga tcaaacgtac ggttcgtggg catgagagta cctcgttgcg
      781 gcgaggcccg agccatggct tcgaacgggg gggataacca agcgagcaac aaccgaaagc
      841 tgggtaactc atgaccaaag attcggcaat ctgtcgccag aatattgtct aatctattcc
      901 aaatagccga cgagttcaag ggcttcgggg atcgttttct cgctgagggc tgcttgtgtg
      961 ccgtgggcga tcctccgagg atgacggcaa gccccctgca acgtagatgc ggaaaaactg
     1021 gcctaaaagc cgtacggagc ggatcgcgct cgaatcgttc tcgcggtgcg cgacgtccat
     1081 gcatccgacc gcggagcgca cgccggagga cgatctccat gtaaccccgc ttgacaagct
     1141 ggattgaagt cggcaaaatt gccagtcatc cggaaaagtc ggcaaccaaa catccgagaa
     1201 cttgattttt ttgccgaaag tcggccatgt ttcggtagag ggcgcgtcgg ccttgccggg
     1261 gaagcccgag ccaacgggaa agaatgccga tatggggcac ggcccaacgg agctgccaag
     1321 gcgacacata tcccatccaa agtgaggtca cggtgatgag ggtgagtaag tcgagttcgc
     1381 aagcaggggt attccgtacc ccttatcgaa gggaggaccg caagcaccgc caccggatgg
     1441 agattctgac cgtcctgcga aaatccgagc agccattatc ttccagcgac atcgcccaaa
     1501 tgctggctgc tgtgggatat atgctcagcg aacggagtat tcgactgtat cttgccgaga
     1561 tgtgcgaaga agggctaacc atctctcacg gccaccgtgg ccatacgatc tctgaaaagg
     1621 gtttggctga gctgcgtgtg gcgcagacca tccagcgtgt cggttatcta tccgcccgaa
     1681 ttgatgaaat gacctacaaa atgacctttg acctggagcg ccgcgcgggc accgtggttg
     1741 tcaatacctc tgtggtcaat ccccatgatc ttgctgtttg cctggatagc gtttgccagg
     1801 tgttcaacaa aggcttcgct atgggcagcc gcttggcact gctagagccc ggcgagcgga
     1861 ttggtgagct acagattccg cctggccgaa ttgggttttg taccatttgt tcgattaccc
     1921 tcaacagtgt cctcttaaaa catggtgttc ccacaacctc tcgcttcggc ggcttactag
     1981 aagtccgcga tggtaaacct ttgcgttttg ccgagatcat tcactacgat gggaccagta
     2041 tcgatcctct ggaagtcttc atccgcggac ggatgaccga ctatcgtggc gccattgcca
     2101 ccggcaacgg actgatcggg gccagtttcc gcgaaatccc ggaagacgcc cgcgatttgg
     2161 cccagcgtct gagtgacgaa atggggcgga ttgggttggg gggtgtcatg gcgattgg
//
LOCUS       DAOSUT010000287         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_20241_length_2189_cov_5.830834, whole genome shotgun sequence.
ACCESSION   DAOSUT010000287 DAOSUT010000000
VERSION     DAOSUT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20241_length_2189_cov_5.830834"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            592..1662
                     /locus_tag="PLD05_15640"
     CDS             592..1662
                     /locus_tag="PLD05_15640"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOP78918.1"
                     /translation="MGSRFSTRKSRQSGLCWGTALRVAAIALVLVGWVLADPTGTAVA
                     EALNPPAFSTIDVLPEGERTWLRDAVAYQISMGRSLDMAVREIKALKSFPEQWSEPQL
                     AALVGAMYQRLTAPPQRPSRRPRALALIGDRYHDPAYIRPPLEEAMEKAGVAVAFLYD
                     VRQLVPQVVFQYDLLVILRDGMLWPDPQPNGSFGKPVFWLTEEQEMTVASFVWAGGGF
                     LALHNATALKALNETESLYLRVLGASYAGHGPAQEGYYVRVLNGSHPVTAGVHDYFVE
                     DERHWPRLYVSDALVLLESASGEETSLHGFVRTFGLGRVCYLANGHNRRVLESDVMQR
                     ILRNAARWCLEPRLDVVENLER"
     gene            complement(1937..>2189)
                     /locus_tag="PLD05_15645"
     CDS             complement(1937..>2189)
                     /locus_tag="PLD05_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78919.1"
                     /translation="IPVFPVVIRFARPLTNNGGSTLGVYPKTIPSVLTTGGRNDHSQD
                     WMGRASPKRSVRESMPSFFSSCLLQISDRCGRLSLLEKF"
BASE COUNT          444 a          599 c          652 g          494 t
ORIGIN      
        1 gcccatcggg agtttaccct tccactgcat aaggaacggc acgcggatgc cccctcctgc
       61 cagaggcccg gaaggggatt gagaagcacc gcatgctcgg cgggacgtac tccgagatcg
      121 tcgagagcta cctcgcggaa tcggcgcccc taagttgctt cccacaccac tgcacctgcc
      181 cagcgcaatc ggccagccga tcgctagtgt ttcaccccaa ccgccgggac acatgcattg
      241 aggcgaaggc cccggttttc cgggcacgga atggaagatt ccgactgcga aatttgtaag
      301 ccgacctttc cggaattaca ctccaagaaa gatagatggg gtttttgggg cttcttttcg
      361 agcacggcgg cccgtgtttc gccctaggtt tgggattgcg aatgcccgcc catcgggacc
      421 ggtcgcaatt cttttcggcg acctgtctgt tgattccata cgatgacttc acgtggtcgt
      481 gtgatcgatc agtcgcaaaa ggagtcgcga agatttctga tcccaagtcg ctaaaaaacg
      541 gtgttgcagt tccgctacgc gtttctcgag cttcgcacca ggagacatat catgggttcc
      601 cgattctcaa ctcgaaagag taggcagtca ggactgtgtt ggggcacggc gctccgcgtt
      661 gcggcgatcg cgttggtgct tgtggggtgg gttttggcgg atccaacggg caccgccgtt
      721 gccgaggcgt taaatccacc ggctttctcg accatcgatg tattgcccga gggagagcga
      781 acctggctgc gagatgcggt agcataccaa atatccatgg gtcgctcact tgatatggca
      841 gtccgtgaaa taaaggctct gaagtctttc cccgaacaat ggtctgagcc tcaattggcc
      901 gctctcgtgg gggcgatgta tcaaaggctg acggctccgc ctcaaaggcc gtcgcggcgt
      961 ccccgggccc tggccctgat cggcgaccgg tatcacgatc cggcctatat tcgaccaccg
     1021 ctggaggagg ccatggaaaa ggcgggggtg gcggttgcgt ttctgtacga cgttcggcaa
     1081 cttgtgccgc aggttgtgtt tcagtacgat ttgctggtca tccttcggga tgggatgctt
     1141 tggcccgatc cgcaaccgaa cggttctttc ggcaaaccgg tcttctggct tacggaagag
     1201 caagagatga ccgtggcctc attcgtgtgg gccggcggtg gattccttgc gctgcacaac
     1261 gcaacggcct tgaaagcctt aaacgaaaca gaatcgctct atcttcgcgt cctcggtgcg
     1321 agttatgccg gccacggccc tgcccaagag ggctattacg ttcgtgtctt gaacggctcg
     1381 catcccgtga cggcgggggt ccacgactat ttcgtggagg acgagcgcca ctggccgcgc
     1441 ttgtatgttt cggacgccct tgttttgctg gagtccgcct cgggtgaaga gacttcctta
     1501 catggttttg ttcgcacgtt tggtttgggt agggtatgct acctcgcaaa tggacacaac
     1561 cgacgtgtct tggaaagtga cgtcatgcaa cggatacttc gcaacgccgc tcgctggtgc
     1621 ctcgagccgc ggttggacgt cgtggagaat ctcgagcggt aggcatcgag gaatgagaat
     1681 cactaaatgg ttcccgtcac agatagtcgc tcgagtgatg gcggggtcgg atagtctcgt
     1741 cttaccgcga acccaataac tccgcccttc cagccggcaa cgcggggtgt tgcaatcggc
     1801 gggagggcag gaagttcaca taatgggatc cgttggttta cgtggaccaa ccgttgcagt
     1861 gaagcgtccg gattcccgca ttttacgatt tcacctctgg gcaggtttac ggatagagcc
     1921 aagtaagtaa cgcatattaa aatttctcca aaagactgag tctgccgcaa cgatcgctga
     1981 tctggaggag gcacgagctg aagaaagaag gcatactttc gcgaaccgat cgcttcgggg
     2041 aagcccggcc catccagtct tgagaatggt cattcctgcc gccggttgtc agaacggaag
     2101 ggattgtctt aggatacact cctaatgtac tcccaccgtt gttcgtcagc ggccgggcga
     2161 atctgatcac caccggaaaa accggtatg
//
LOCUS       DAOSUT010000288         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_20771_length_2146_cov_10.139168, whole genome shotgun
            sequence.
ACCESSION   DAOSUT010000288 DAOSUT010000000
VERSION     DAOSUT010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20771_length_2146_cov_10.139168"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1477)
                     /locus_tag="PLD05_15650"
     CDS             complement(56..1477)
                     /locus_tag="PLD05_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOP78920.1"
                     /translation="MTRVSQLCVGMLVLLFVFATRAGNTFAEPTAADRPNVLFLLADD
                     LGWGDLSCYGNRKFRTPNLDRLAREGIIFTQYYQAGSVCSPTRAALMTGRYPAELAIH
                     GHLATPEQNAARDMPNFLNPQVPTLPQLLKKAGYTTIHVGKWHLGEPSPETEGLQTYG
                     FDVARWIDCREGDRNLWNIQERPVASRELVDATIQALKEVKDRSFYCQLWFNDPHALL
                     APSVEQMAPLRRQVPEGFTSPFMVYAATVMEMDRQIGRLLNALDELGLRENTLVIFSS
                     DNGPEDIDIANASWSAYGSAGPLRGRKRSLYEGGIRVPFIVRWPAGTPSGLVNNDTVI
                     CGVDFLPTICELAGVALPEDVKATCRGESMVSAIRGDRTAQRRTTLFWEWRFRIANHP
                     WNRSPILAVREGPYKLLFNPDGSRVELYEIPKDSGEYNNIAEKKPEIVKQLMEKGLAW
                     QKTLPPGRFDKDAGQADYPWPTE"
     gene            complement(1694..>2146)
                     /locus_tag="PLD05_15655"
     CDS             complement(1694..>2146)
                     /locus_tag="PLD05_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78921.1"
                     /translation="IAPEGSTDTHGPNWGNTNTWTGILAPGLSVRNILDALANRRCYS
                     TRDRNCRFQFSINGSPMGTIITTPIRTAKLTIQLNDPDDNDVITKVELFQDGEQLEVF
                     EPNQKDVLLEKTYTPSEGKHYIWAKVTQADGNLLWGAPIWFIASGKAE"
BASE COUNT          464 a          603 c          572 g          507 t
ORIGIN      
        1 gcggcgaatg cccgattgcc gctgccagtc gctcaaagtg cttaggactt tccgcctatt
       61 ccgttggcca gggataatcg gcctgaccgg catctttgtc gaatcgccca ggcggaagtg
      121 tcttctgcca ggctagacct ttctccatga gttgtttgac aatctcgggc tttttctccg
      181 caatgttgtt atactcgcca gagtccttcg gtatttcgta gagttccaca cgcgagccat
      241 cgggattgaa aaggagtttg tagggacctt cccgaaccgc caggatgggg ctgcggttcc
      301 aagggtggtt ggcaatccga aatcgccatt cccaaaagag cgttgtccgc ctttgcgcgg
      361 ttctatcccc tcgaatggcc gaaaccatgc tttcacctcg gcatgtggct ttgacgtctt
      421 caggcaatgc cacgccagcc agttcacaaa tggtgggcaa gaaatcgaca ccgcagatca
      481 ccgtgtcgtt gttaaccagc ccgctgggtg tcccggcagg ccagcggaca atgaatggaa
      541 cgcgaattcc cccttcgtac aagcttcgct ttcgtccccg caaaggtcct gcacttccat
      601 aggctgacca cgaggcattg gcgatgtcga tatcctccgg cccgttatcg gaggaaaaga
      661 tgaccaaggt gttctccctc agcccaagtt cgtccagggc attgaggagc cgaccgattt
      721 gacggtccat ttccatcacc gtagcagcat acaccatgaa ggggctggtg aatccttctg
      781 ggacctgcct tcgcaatggg gccatttgct cgacagaggg cgccaataac gcatgcggat
      841 cattgaacca caactggcag tagaacgaac gatctttaac ctcctttagg gcttggattg
      901 ttgcgtcaac gagctcccgc gatgccaccg gacgttcctg aatattccaa agatttcggt
      961 caccttcccg gcagtcgatc catcgggcta cgtcgaaccc ataggtctgc aatccttccg
     1021 tctcgggcga gggctcgccc aggtgccatt ttcccacgtg aattgtcgta tatcctgcct
     1081 ttttcaaaag ttgcggaaga gtcggaactt ggggattgag gaaattgggc atatcgcggg
     1141 ccgcgttttg ctcgggagtc gccaagtgac catggatcgc caactccgcc gggtaacgac
     1201 ccgtcatcag tgccgcccgc gttggtgaac acacggagcc ggcctgatag tactgggtga
     1261 aaataattcc ctcccgggct aaccgatcaa gatttggcgt gcgaaacttt cggttaccgt
     1321 agcagctcag gtctccccaa cccaggtcgt cagccagcag aaagaggaca ttcgggcgat
     1381 ctgcggctgt cggttccgca aaggtattcc ccgccctggt tgcaaaaaca aatagaagca
     1441 ccaacattcc cacgcataac tgactgacgc gcgtcatggt tgtacctccc cataatggtt
     1501 tgacagagaa cccaacagcc gaattatcaa gcttagagcc tttcccatga tctcccctca
     1561 cccgctgggc gggagggggc cagggatgag ggtggcgcga atcgctgccg attttctcga
     1621 tcaactccga cgttcgcttg ccctcacccc aaccctctcc cagagggaga gggagacttt
     1681 ttgggataca ttcttattcc gccttgccag aagcaatgaa ccagatcggg gcgccccaca
     1741 aaagattccc gtcggcttgg gtcaccttgg cccaaatata gtgctttcct tctgacggcg
     1801 tataagtctt ttccagaagt acatcctttt gatttggctc aaaaacctcg agttgttcgc
     1861 catcttgaaa cagctcgacc ttggtgatga cgtcgttgtc gtcaggatca ttaagttgga
     1921 tagtcaactt ggctgttcgg ataggagttg tgatgattgt gcccattggc gacccgttga
     1981 tgctaaattg gaaccgacag ttgcggtcgc gcgtggagta acatcggcga ttggccaggg
     2041 catccaagat atttcgaaca gaaagaccgg gtgccagaat cccggtccag gtattggtat
     2101 ttccccaatt tggaccgtgg gtatcagtgg aaccttctgg cgcgat
//
LOCUS       DAOSUT010000289         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_25575_length_1853_cov_5.620133, whole genome shotgun sequence.
ACCESSION   DAOSUT010000289 DAOSUT010000000
VERSION     DAOSUT010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25575_length_1853_cov_5.620133"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..683)
                     /locus_tag="PLD05_15660"
     CDS             complement(345..683)
                     /locus_tag="PLD05_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOP78922.1"
                     /translation="MTGLQDQDIFGKPINLANLLSVQPKAIVSRILVKHSGGSITLFA
                     FDAGQAVSEHTAPFDAMIHVLEGTADVTVGQDKHTVNAGKSILLPANVPHAVRAPQAF
                     KMLLFMLRKD"
     gene            1184..1672
                     /locus_tag="PLD05_15665"
     CDS             1184..1672
                     /locus_tag="PLD05_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOP78923.1"
                     /translation="MLSPKMLQALNDQINAELYSSYLYLAMAARCEALDLPGAAHWLR
                     LQSKEEHEHAMKFFQYVNERRGEVKLQAIKEPPCSWNKLCQLFEDVLAHEQYISKRIN
                     DLVKLARDENDYATENFLQWFVKEQVEEEATVDAVIKRLRLVGEMPQGLFFIDRELGQ
                     RS"
     gene            complement(1641..>1853)
                     /locus_tag="PLD05_15670"
     CDS             complement(1641..>1853)
                     /locus_tag="PLD05_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOP78924.1"
                     /translation="LPRPWGRLSLFRPNGWIGNLPESAVACKQKTAQNHLDPAPLRQL
                     RVKDQQVFTGRANAIALNFAGPVLDR"
BASE COUNT          440 a          477 c          528 g          408 t
ORIGIN      
        1 ggatggtagg ggaatgggca aaatacggcg tgtcggaaaa atgcgtcaga gatgcatgcg
       61 ccgttatgcg tatcggagga atacctgtga gatattgggc aaatcacgac gagttcgtaa
      121 tgtgcgcaat tagtaagata gagagacttt tcggagcacc tcctaggctt ggcgtccctt
      181 attatttccc ttaccgggga aaggggcgtc gaaaccgacc accttgccgt gaaatgtcat
      241 aggttttgga gattaggagg ctacgaaaaa agaactgtcc gccggtggcc tcccagaagg
      301 tcgagggcac caacaaaaac aagcggacca agatcgcaag cggttcagtc tttccggagc
      361 atgaagagga gcatcttgaa ggcctggggg gccctaaccg cgtgagggac gttggctggt
      421 agcaagatcg actttccggc gttgaccgtg tgcttgtcct ggcccacggt cacatccgcg
      481 gtgccctcta ggacgtggat catggcatca aaaggggctg tatgttctga cactgcctgc
      541 ccggcatcaa aggcaaagag cgtgattgac ccgcctgagt gtttgacaag aatccgacta
      601 acaatggcct ttggctggac actcaaaaga ttggccaaat taatcggctt gccgaatatg
      661 tcttggtcct ggagcccggt cataagaaag tcctcgcatt tcctcgtctt tgctcttgcg
      721 atcagtatgt taggtacggg ccaagcacgc cggaacaact cagaatatgc tccgtatccc
      781 ggcccaagcg gagcgaaccc tgcaaacggt acgtggtatt atatctggcc tggaaagcac
      841 gccaacggtt tgatcgaaac aagggtcatg attgggggaa aagcgtaggc gttatcgcgg
      901 tccccaacgg tttagaaact ggagcgacat tccccgaatt actctccctc ccgactccct
      961 caagcaggcc ttgtctgtcc ctgttggccc tagacctatc cccggctgtg ggctacccga
     1021 aatggccggg tcccatgaaa ccggccaggc ttgccttcct ttgaggtttg gtaataattc
     1081 atgaatttcc cgtcgttgca gcggaggggc caattgggta aaaagaccct tcgcagcatt
     1141 tcccatcgat tgacaaaacg gtgataacgg aaaggagtgt cccatgttaa gccccaagat
     1201 gttgcaggct ttgaacgatc agatcaatgc cgagctctat tcctcgtacc tttacctggc
     1261 gatggccgcc cgctgtgaag cccttgacct tcccggggcc gctcactggt tgcgtctcca
     1321 atccaaggaa gagcacgagc acgccatgaa gttctttcag tacgtgaacg aacggcgtgg
     1381 tgaggtcaag ttgcaggcca ttaaggagcc gccgtgttct tggaacaagc tttgccaact
     1441 ctttgaggat gtcctcgctc acgagcaata catcagcaag cggatcaacg atctggttaa
     1501 attagctcgg gacgaaaacg actacgccac cgagaatttc ctgcagtggt tcgtgaaaga
     1561 gcaagtggag gaagaggcca ccgtggatgc cgtcatcaag cggctccgac tggtcggaga
     1621 gatgccccag gggctgttct tcatcgatcg agaactgggc cagcgaagtt gagggctatc
     1681 gcgttcgctc ggccggtaaa aacctgttga tctttaacac gcaattgcct taacggcgca
     1741 ggatcgagat ggttctgcgc cgttttttgt ttacaagcca ccgcggattc gggcaagttg
     1801 ccgatccagc cgttggggcg aaacagggat agccgtcccc aaggcctggg caa
//
LOCUS       DAOSUT010000290         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_27057_length_1782_cov_8.365953, whole genome shotgun sequence.
ACCESSION   DAOSUT010000290 DAOSUT010000000
VERSION     DAOSUT010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27057_length_1782_cov_8.365953"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLD05_15675"
     CDS             <1..780
                     /locus_tag="PLD05_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOP78925.1"
                     /translation="NVPVDLVIIRENTEDLYAGVEFERGKPATSQLIDCINQLSTDRK
                     IKTPAAETGISIKPISVSGTERIVRYAFRYARENGRKKVTAVHKANIMKFTDGLFLEV
                     ARQVATEYPDIEFEDRIVDNMCMQLVQKPELYDVLVLPNLYGDIISDLAAGLVGGLGV
                     APGANIGENGAVFEATHGSAPKYKGQNKVNPTALILSAVLMLRYLGEKEAADRLEWAV
                     AEVIREGKDVTYDLKPHRDDPTAVGTQEMADAICRKLRQAV"
BASE COUNT          403 a          480 c          510 g          389 t
ORIGIN      
        1 aacgtgccgg tcgatcttgt cattatccgc gagaacaccg aggaccttta cgcgggcgtc
       61 gagtttgaac gtggaaaacc cgcaaccagc cagttgattg actgtatcaa ccagttatcg
      121 acagaccgaa agatcaagac accggccgca gagacgggca tttccatcaa gccgattagt
      181 gtctcgggaa cagagcgaat cgttcgctac gctttccgct atgcccggga aaatggccga
      241 aaaaaggtca ctgccgtgca caaggcgaat atcatgaaat tcactgacgg cctcttcctt
      301 gaggtggcac ggcaagtggc aacagagtat cccgacatcg aattcgaaga ccgaatcgtc
      361 gataacatgt gcatgcaatt ggtccagaag cccgagttgt atgacgtcct tgtcctaccg
      421 aatctctacg gtgacatcat tagcgacttg gccgccggac ttgtcggtgg tttgggagtc
      481 gcgccggggg ccaatattgg ggaaaacggg gccgttttcg aggccaccca cggcagtgcc
      541 ccgaagtaca agggccaaaa caaggtcaac ccaacggcgt tgatcctgtc ggccgttttg
      601 atgcttcgtt atctcggcga aaaagaggcg gcagatcgct tggaatgggc cgtggccgag
      661 gtcatccgcg agggcaagga tgtgacctat gacttgaaac ctcatcggga cgacccaacg
      721 gccgtgggga cccaggaaat ggccgacgcc atttgtcgca agctccgcca agcggtctag
      781 ccggccgtgg actctcctgc gtataggtcg aattggtttc acatccggag caaacggtag
      841 attcggacca gtccggacga taatggttta agagcgtatc ccaaaaagta cccccctctc
      901 cctccgggag agggttgggg tgagggcaag cggtcgtcgg aatcgattga gaaaaccagc
      961 agcgattcga gccaccctca cccccgaccc ctctcccgcc gaacgggcga ggggagaatt
     1021 acgggatagg ctctaagcat gcctttaact cctttccgag taagtgctta attcccgggt
     1081 agtggagtgc gcagcaattg tgactgcgct gcgcaggcaa aaagactgcg cgcgggcggc
     1141 ttttcaatcc ctctgctcct aagctctgtt gatttatctc ttatttagaa aacaacttag
     1201 gccggaagtt cccttcttgg catcagatta gcttaaacag ccaacaggcg agttggttgg
     1261 gtcagtccaa tattcggtct cctggaggaa aggaggacgt catgcgtggg aaatgggttg
     1321 cttcagtttt ggtcggtttg ggcgcttgcc tgggagtgtc agtctgggcg aatggtggac
     1381 aggcttatgg ccacgacagc agtagtatga gtattcaact tgtgggacac ggctactata
     1441 gctcttacgg ttatgggcgg tcatacagtt acggatacgg atggcatcat ccaccggttg
     1501 ttcgcccgcc ggcagttgtg gttccatttc cgggtcatcc gcccgtggta attacacccc
     1561 cgacatcgcg accgagttgc tcaaaacctt cgcaccacta cgactaccgc cgcgggtaca
     1621 gctcaggcca cggctcgatc agtatttacg gcccaagagt tggcttctcg atcgcgtggt
     1681 aaacctttca tcgcggctag aggaactaag actttccagc acgagttgag ccgcctgccc
     1741 cctgtggcaa cgggcaaggt ctccgagacc ttgcccgttt tt
//
LOCUS       DAOSUT010000291         1760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_2182
            NODE_27535_length_1760_cov_8.905572, whole genome shotgun sequence.
ACCESSION   DAOSUT010000291 DAOSUT010000000
VERSION     DAOSUT010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864249
            Sequence Read Archive: SRR4236673
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:08:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,137
            CDSs (total)                      :: 3,097
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27535_length_1760_cov_8.905572"
                     /isolate="AMR_MDS_2182"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="49.25 N 123.1 W"
                     /collection_date="2015-06-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLD05_15680"
     CDS             <1..214
                     /locus_tag="PLD05_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOP78926.1"
                     /translation="AMGIATVALWLADFLITQTAPMMYEAWGPAYAFWSYAFLCVVCF
                     FFVLAFLPETKGKTLEEIERSFLARK"
     gene            544..>1760
                     /gene="xseA"
                     /locus_tag="PLD05_15685"
     CDS             544..>1760
                     /gene="xseA"
                     /locus_tag="PLD05_15685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOP78927.1"
                     /translation="MGEDFINSFTAFNMSRDLPVVSVSQLTTMIKEVLEGVFPRVAVI
                     GEITDLSVAKSGHCYLTLKDEGAQLPCVIWRSYFTQIPFELRDGLEVLCRGRLNVYPP
                     HGRYQFIIERIELGGWGAAELALRQLKERLAKEGLFDEARKRPLPKYPRWIAVVTSPS
                     GAAVHDFLTALRERFCPANVLIVPVRVQGEGAAEEIAQAVRRVNDLALPIDVIVVTRG
                     GGSAEDLGAFNTEAVVRAIATSRIPVVSAVGHDIDVTLADLAADVRALTPTDAAFKVI
                     PSREEVTEAVNALAVRLRSSAELRLRMGQAMFERTANHPFFRRPKDWLYEKVQTLDEW
                     DKRLDRAGHLIIERFSQQLQQKITHLESLSPLAVLARGYSMTFVHPDGPLVVEASALR
                     PGQQIKTRFAQGSA"
BASE COUNT          359 a          489 c          495 g          417 t
ORIGIN      
        1 ggccatgggg atcgccacag tagcactctg gttggccgat ttcctcatca cccagacggc
       61 ccccatgatg tatgaagctt ggggaccggc ctacgccttt tggtcgtacg cctttttgtg
      121 tgtcgtctgt ttcttctttg ttttggcatt tctgcccgaa accaaaggaa agacactgga
      181 ggaaatcgaa cggagctttc tcgctcgaaa atgaaggtgc ctatatctca acagaccgaa
      241 aaacgctctc gatcttgtga gagcctagcc caaagttccc tcctcctctg ggagagggtt
      301 ggggtgaggg caagccattg ccaaagtcga tcaagaaaac cggcagcgat tcgagccacc
      361 cccaccctcg acccctctcc cgtggaacgg gagacgggag actatgggat aggtttttaa
      421 tcccagtgag tcctgcaaac tgggcaaacc gtcgggtgaa atgcacgttt tcctttactt
      481 gtgtcacatc ggtcagaaat aattaccgcg aacatggtcg gtagttccac gcgaggagtt
      541 tctatgggcg aggatttcat aaactctttc accgccttca atatgtcgcg cgacttgcct
      601 gtggtcagcg tttcccagtt gaccacgatg atcaaggagg tcttagaagg cgtattcccc
      661 cgggtggccg ttattggtga aattacggac ctctcggttg cgaagtccgg tcactgttat
      721 ttgaccctca aagacgaggg ggcgcagctt ccctgcgtga tttggcggtc ctactttacg
      781 caaataccct ttgagcttcg ggacggtctg gaggtccttt gtcgcggacg actgaatgtg
      841 tatccgcccc acggacgtta ccagttcatt attgaacgca tcgagttagg tggctgggga
      901 gctgctgaat tggccctccg ccagttgaaa gaacgtctcg ctaaagaagg tctttttgac
      961 gaagcgcgaa aacgtcccct tcccaaatat ccgcgttgga ttgccgtcgt gaccagtccc
     1021 agtggcgcag ccgttcatga ttttctgacg gctttgcggg aacgtttctg tccggcaaac
     1081 gtgttgattg taccggttcg cgtccaggga gaaggagcag cggaggaaat cgcccaggct
     1141 gtccggcggg tgaacgacct cgcgcttcca attgatgtca ttgttgtgac ccgcggcggt
     1201 ggcagcgccg aggaccttgg ggccttcaac accgaggcgg tcgttcgcgc catcgccaca
     1261 tcgcgaattc ccgtcgtctc ggccgtgggc catgatatcg atgtcacgtt ggcggacctg
     1321 gcggcggatg ttcgtgccct gactccaacc gacgccgctt ttaaagtgat tccgtcccgg
     1381 gaagaggtca cggaagcggt caacgccctg gccgtccgtt tgcgatcgag tgccgaattg
     1441 cgattacgga tggggcaagc catgttcgaa cggacggcaa accatccttt ctttcgccgt
     1501 cccaaggact ggctttacga gaaagtccag acgctcgatg agtgggacaa acgcctggat
     1561 cgtgcgggcc atttgatcat cgaacggttt tcacaacagt tgcaacaaaa gatcacccac
     1621 ctggaatccc tcagtccttt ggctgtgctt gcccggggct attccatgac atttgtccac
     1681 cccgatggcc ctcttgtcgt tgaggcgtcg gcccttcgcc cagggcagca gatcaaaacc
     1741 cgctttgccc aaggcagtgc
//
